LOCUS       DAJVRF010000001        85488 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJVRF010000001 DAJVRF010000000
VERSION     DAJVRF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 85488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85488
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.001"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(74..1010)
                     /gene="ldtA"
                     /locus_tag="OQN13_000001"
                     /pseudo
     CDS             complement(74..1010)
                     /gene="ldtA"
                     /locus_tag="OQN13_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            complement(1072..2151)
                     /gene="cobT"
                     /locus_tag="OQN13_000002"
     CDS             complement(1072..2151)
                     /gene="cobT"
                     /locus_tag="OQN13_000002"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCS1613485.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(2163..2906)
                     /gene="cobS"
                     /locus_tag="OQN13_000003"
     CDS             complement(2163..2906)
                     /gene="cobS"
                     /locus_tag="OQN13_000003"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCS1613486.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(2903..3448)
                     /gene="cobU"
                     /locus_tag="OQN13_000004"
     CDS             complement(2903..3448)
                     /gene="cobU"
                     /locus_tag="OQN13_000004"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCS1613487.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(3582..4019)
                     /locus_tag="OQN13_000005"
     CDS             complement(3582..4019)
                     /locus_tag="OQN13_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HCS1613488.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(4237..4412)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(4501..5177)
                     /locus_tag="OQN13_000006"
                     /pseudo
     CDS             complement(4501..5177)
                     /locus_tag="OQN13_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(5380..6189)
                     /gene="pduF"
                     /locus_tag="OQN13_000007"
     CDS             complement(5380..6189)
                     /gene="pduF"
                     /locus_tag="OQN13_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HCS1613489.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
     gene            6701..6985
                     /gene="pduA"
                     /locus_tag="OQN13_000008"
     CDS             6701..6985
                     /gene="pduA"
                     /locus_tag="OQN13_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HCS1613490.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            6982..7794
                     /gene="pduB"
                     /locus_tag="OQN13_000009"
     CDS             6982..7794
                     /gene="pduB"
                     /locus_tag="OQN13_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HCS1613491.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQDTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDHPSYI"
     gene            7813..9477
                     /gene="pduC"
                     /locus_tag="OQN13_000010"
     CDS             7813..9477
                     /gene="pduC"
                     /locus_tag="OQN13_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HCS1613492.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTNGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGSFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            9487..10155
                     /locus_tag="OQN13_000011"
     CDS             9487..10155
                     /locus_tag="OQN13_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HCS1613493.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            10170..10688
                     /gene="pduE"
                     /locus_tag="OQN13_000012"
     CDS             10170..10688
                     /gene="pduE"
                     /locus_tag="OQN13_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HCS1613494.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            10698..12530
                     /locus_tag="OQN13_000013"
     CDS             10698..12530
                     /locus_tag="OQN13_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HCS1613495.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            12520..12870
                     /locus_tag="OQN13_000014"
     CDS             12520..12870
                     /locus_tag="OQN13_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HCS1613496.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            12890..13165
                     /gene="pduJ"
                     /locus_tag="OQN13_000015"
     CDS             12890..13165
                     /gene="pduJ"
                     /locus_tag="OQN13_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HCS1613497.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            13189..13611
                     /locus_tag="OQN13_000016"
     CDS             13189..13611
                     /locus_tag="OQN13_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HCS1613498.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            13614..14246
                     /locus_tag="OQN13_000017"
     CDS             13614..14246
                     /locus_tag="OQN13_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HCS1613499.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            14243..14734
                     /gene="pduM"
                     /locus_tag="OQN13_000018"
     CDS             14243..14734
                     /gene="pduM"
                     /locus_tag="OQN13_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HCS1613500.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            14738..15013
                     /gene="pduN"
                     /locus_tag="OQN13_000019"
     CDS             14738..15013
                     /gene="pduN"
                     /locus_tag="OQN13_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HCS1613501.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            15025..16032
                     /gene="pduO"
                     /locus_tag="OQN13_000020"
     CDS             15025..16032
                     /gene="pduO"
                     /locus_tag="OQN13_000020"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HCS1613502.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            16029..17414
                     /gene="pduP"
                     /locus_tag="OQN13_000021"
                     /pseudo
     CDS             16029..17414
                     /gene="pduP"
                     /locus_tag="OQN13_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            17425..18537
                     /locus_tag="OQN13_000022"
     CDS             17425..18537
                     /locus_tag="OQN13_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCS1613503.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLKGLL"
     gene            18534..19879
                     /locus_tag="OQN13_000023"
                     /pseudo
     CDS             18534..19879
                     /locus_tag="OQN13_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
     gene            19882..20436
                     /gene="pduT"
                     /locus_tag="OQN13_000024"
     CDS             19882..20436
                     /gene="pduT"
                     /locus_tag="OQN13_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HCS1613504.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            20436..20786
                     /gene="pduU"
                     /locus_tag="OQN13_000025"
     CDS             20436..20786
                     /gene="pduU"
                     /locus_tag="OQN13_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HCS1613505.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            20791..21234
                     /locus_tag="OQN13_000026"
     CDS             20791..21234
                     /locus_tag="OQN13_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HCS1613506.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(21335..21664)
                     /locus_tag="OQN13_000027"
     CDS             complement(21335..21664)
                     /locus_tag="OQN13_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCS1613507.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(21836..22894)
                     /locus_tag="OQN13_000028"
     CDS             complement(21836..22894)
                     /locus_tag="OQN13_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS1613508.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(23092..23565)
                     /gene="sbmC"
                     /locus_tag="OQN13_000029"
     CDS             complement(23092..23565)
                     /gene="sbmC"
                     /locus_tag="OQN13_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HCS1613509.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(23684..24850)
                     /gene="dacD"
                     /locus_tag="OQN13_000030"
     CDS             complement(23684..24850)
                     /gene="dacD"
                     /locus_tag="OQN13_000030"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HCS1613510.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVFSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            25059..26486
                     /gene="sbcB"
                     /locus_tag="OQN13_000031"
     CDS             25059..26486
                     /gene="sbcB"
                     /locus_tag="OQN13_000031"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCS1613511.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(26529..26756)
                     /locus_tag="OQN13_000032"
     CDS             complement(26529..26756)
                     /locus_tag="OQN13_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCS1613512.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(26770..27828)
                     /gene="yeeE"
                     /locus_tag="OQN13_000033"
     CDS             complement(26770..27828)
                     /gene="yeeE"
                     /locus_tag="OQN13_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCS1613513.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(28007..29365)
                     /gene="plaP"
                     /locus_tag="OQN13_000034"
     CDS             complement(28007..29365)
                     /gene="plaP"
                     /locus_tag="OQN13_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCS1613514.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(29355..29417)
                     /gene="yoeI"
                     /locus_tag="OQN13_000035"
     CDS             complement(29355..29417)
                     /gene="yoeI"
                     /locus_tag="OQN13_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCS1613515.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(29632..30561)
                     /locus_tag="OQN13_000036"
     CDS             complement(29632..30561)
                     /locus_tag="OQN13_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1613516.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(30607..31431)
                     /locus_tag="OQN13_000037"
     CDS             complement(30607..31431)
                     /locus_tag="OQN13_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1613517.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(31514..31768)
                     /gene="yoeB"
                     /locus_tag="OQN13_000038"
     CDS             complement(31514..31768)
                     /gene="yoeB"
                     /locus_tag="OQN13_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCS1613518.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(31765..32016)
                     /gene="yefM"
                     /locus_tag="OQN13_000039"
     CDS             complement(31765..32016)
                     /gene="yefM"
                     /locus_tag="OQN13_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCS1613519.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            32299..32349
                     /gene="hisL"
                     /locus_tag="OQN13_000040"
     CDS             32299..32349
                     /gene="hisL"
                     /locus_tag="OQN13_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCS1613520.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    32325..32448
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            32495..33394
                     /gene="hisG"
                     /locus_tag="OQN13_000041"
     CDS             32495..33394
                     /gene="hisG"
                     /locus_tag="OQN13_000041"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCS1613521.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            33400..34704
                     /gene="hisD"
                     /locus_tag="OQN13_000042"
     CDS             33400..34704
                     /gene="hisD"
                     /locus_tag="OQN13_000042"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCS1613522.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            34701..35771
                     /gene="hisC"
                     /locus_tag="OQN13_000043"
     CDS             34701..35771
                     /gene="hisC"
                     /locus_tag="OQN13_000043"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCS1613523.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCESGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            35771..36838
                     /gene="hisB"
                     /locus_tag="OQN13_000044"
     CDS             35771..36838
                     /gene="hisB"
                     /locus_tag="OQN13_000044"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCS1613524.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            36838..37428
                     /gene="hisH"
                     /locus_tag="OQN13_000045"
     CDS             36838..37428
                     /gene="hisH"
                     /locus_tag="OQN13_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCS1613525.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            37428..38165
                     /gene="hisA"
                     /locus_tag="OQN13_000046"
     CDS             37428..38165
                     /gene="hisA"
                     /locus_tag="OQN13_000046"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCS1613526.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            38147..38923
                     /gene="hisF"
                     /locus_tag="OQN13_000047"
     CDS             38147..38923
                     /gene="hisF"
                     /locus_tag="OQN13_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCS1613527.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            38917..39528
                     /locus_tag="OQN13_000048"
     CDS             38917..39528
                     /locus_tag="OQN13_000048"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCS1613528.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(39625..40602)
                     /gene="wzzB"
                     /locus_tag="OQN13_000049"
     CDS             complement(39625..40602)
                     /gene="wzzB"
                     /locus_tag="OQN13_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCS1613529.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLAQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(40748..41914)
                     /gene="ugd"
                     /locus_tag="OQN13_000050"
     CDS             complement(40748..41914)
                     /gene="ugd"
                     /locus_tag="OQN13_000050"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCS1613530.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(42164..43570)
                     /gene="gndA"
                     /locus_tag="OQN13_000051"
     CDS             complement(42164..43570)
                     /gene="gndA"
                     /locus_tag="OQN13_000051"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCS1613531.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(43734..45104)
                     /gene="cpsG"
                     /locus_tag="OQN13_000052"
                     /gene_synonym="manB"
     CDS             complement(43734..45104)
                     /gene="cpsG"
                     /locus_tag="OQN13_000052"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCS1613532.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPFEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(45209..46645)
                     /gene="cpsB"
                     /locus_tag="OQN13_000053"
     CDS             complement(45209..46645)
                     /gene="cpsB"
                     /locus_tag="OQN13_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCS1613533.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(46648..47871)
                     /gene="wcaI"
                     /locus_tag="OQN13_000054"
     CDS             complement(46648..47871)
                     /gene="wcaI"
                     /locus_tag="OQN13_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCS1613534.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(47868..48347)
                     /gene="gmm"
                     /locus_tag="OQN13_000055"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(47868..48347)
                     /gene="gmm"
                     /locus_tag="OQN13_000055"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCS1613535.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(48350..49315)
                     /gene="fcl"
                     /locus_tag="OQN13_000056"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(48350..49315)
                     /gene="fcl"
                     /locus_tag="OQN13_000056"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCS1613536.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(49318..50439)
                     /gene="gmd"
                     /locus_tag="OQN13_000057"
     CDS             complement(49318..50439)
                     /gene="gmd"
                     /locus_tag="OQN13_000057"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCS1613537.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(50465..51013)
                     /gene="wcaF"
                     /locus_tag="OQN13_000058"
     CDS             complement(50465..51013)
                     /gene="wcaF"
                     /locus_tag="OQN13_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCS1613538.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(51029..51775)
                     /gene="wcaE"
                     /locus_tag="OQN13_000059"
     CDS             complement(51029..51775)
                     /gene="wcaE"
                     /locus_tag="OQN13_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCS1613539.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(51786..53003)
                     /gene="wcaD"
                     /locus_tag="OQN13_000060"
     CDS             complement(51786..53003)
                     /gene="wcaD"
                     /locus_tag="OQN13_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCS1613540.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(52978..54195)
                     /gene="wcaC"
                     /locus_tag="OQN13_000061"
     CDS             complement(52978..54195)
                     /gene="wcaC"
                     /locus_tag="OQN13_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCS1613541.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(54192..54680)
                     /gene="wcaB"
                     /locus_tag="OQN13_000062"
     CDS             complement(54192..54680)
                     /gene="wcaB"
                     /locus_tag="OQN13_000062"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCS1613542.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(54683..55522)
                     /gene="wcaA"
                     /locus_tag="OQN13_000063"
     CDS             complement(54683..55522)
                     /gene="wcaA"
                     /locus_tag="OQN13_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCS1613543.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(55615..57777)
                     /gene="wzc"
                     /locus_tag="OQN13_000064"
     CDS             complement(55615..57777)
                     /gene="wzc"
                     /locus_tag="OQN13_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCS1613544.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(57780..58223)
                     /gene="wzb"
                     /locus_tag="OQN13_000065"
     CDS             complement(57780..58223)
                     /gene="wzb"
                     /locus_tag="OQN13_000065"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCS1613545.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(58229..59368)
                     /gene="wza"
                     /locus_tag="OQN13_000066"
     CDS             complement(58229..59368)
                     /gene="wza"
                     /locus_tag="OQN13_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCS1613546.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            59677..59826
                     /locus_tag="OQN13_000067"
     CDS             59677..59826
                     /locus_tag="OQN13_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1613547.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            60027..61610
                     /locus_tag="OQN13_000068"
     CDS             60027..61610
                     /locus_tag="OQN13_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS1613548.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(61884..63737)
                     /gene="asmA"
                     /locus_tag="OQN13_000069"
     CDS             complement(61884..63737)
                     /gene="asmA"
                     /locus_tag="OQN13_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCS1613549.1"
                     /translation="MRRFLTTLMILLVVLVAGLSVLVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(63759..64340)
                     /gene="dcd"
                     /locus_tag="OQN13_000070"
     CDS             complement(63759..64340)
                     /gene="dcd"
                     /locus_tag="OQN13_000070"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCS1613550.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(64432..65073)
                     /gene="udk"
                     /locus_tag="OQN13_000071"
     CDS             complement(64432..65073)
                     /gene="udk"
                     /locus_tag="OQN13_000071"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCS1613551.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            65391..68708
                     /locus_tag="OQN13_000072"
     CDS             65391..68708
                     /locus_tag="OQN13_000072"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCS1613552.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWERELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(68818..69666)
                     /gene="alkA"
                     /locus_tag="OQN13_000073"
     CDS             complement(68818..69666)
                     /gene="alkA"
                     /locus_tag="OQN13_000073"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCS1613553.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            69800..71152
                     /gene="yegD"
                     /locus_tag="OQN13_000074"
     CDS             69800..71152
                     /gene="yegD"
                     /locus_tag="OQN13_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS1613554.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(71165..73105)
                     /gene="yegI"
                     /locus_tag="OQN13_000075"
     CDS             complement(71165..73105)
                     /gene="yegI"
                     /locus_tag="OQN13_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCS1613555.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(73102..73863)
                     /gene="pphC"
                     /locus_tag="OQN13_000076"
     CDS             complement(73102..73863)
                     /gene="pphC"
                     /locus_tag="OQN13_000076"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HCS1613556.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(73860..74519)
                     /locus_tag="OQN13_000077"
     CDS             complement(73860..74519)
                     /locus_tag="OQN13_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCS1613557.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(74740..74799)
                     /gene="ibsA"
                     /locus_tag="OQN13_000078"
     CDS             complement(74740..74799)
                     /gene="ibsA"
                     /locus_tag="OQN13_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCS1613558.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(75072..75128)
                     /locus_tag="OQN13_000079"
     CDS             complement(75072..75128)
                     /locus_tag="OQN13_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1613559.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(75399..75455)
                     /locus_tag="OQN13_000080"
     CDS             complement(75399..75455)
                     /locus_tag="OQN13_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1613560.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            75734..76975
                     /gene="mdtA"
                     /locus_tag="OQN13_000081"
     CDS             75734..76975
                     /gene="mdtA"
                     /locus_tag="OQN13_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCS1613561.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKTLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            76975..80097
                     /gene="mdtB"
                     /locus_tag="OQN13_000082"
     CDS             76975..80097
                     /gene="mdtB"
                     /locus_tag="OQN13_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCS1613562.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            80098..83175
                     /gene="mdtC"
                     /locus_tag="OQN13_000083"
     CDS             80098..83175
                     /gene="mdtC"
                     /locus_tag="OQN13_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCS1613563.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHLLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            83176..84591
                     /locus_tag="OQN13_000084"
     CDS             83176..84591
                     /locus_tag="OQN13_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1613564.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            84588..>85488
                     /gene="baeS"
                     /locus_tag="OQN13_000085"
     CDS             84588..>85488
                     /gene="baeS"
                     /locus_tag="OQN13_000085"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCS1613565.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSM"
BASE COUNT        20908 a        22645 c        21518 g        20417 t
ORIGIN      
        1 gtccagtcag aggagccaaa ttcaaaaaag cctgctttct agcaggcttt ttgctttcta
       61 attaccaacg ctcttaaaac atctgtcttg aaccagaact aatttgcaca ggcattcccg
      121 atcgacgttg caacgcagca tttgcgcgat ttacatcaac ttcttgcccg ctgataaacg
      181 cccgcaaaga tggggttacc ggcaatggca cttttcggtc agactcatat tctgcacgat
      241 tgcgcgacaa cggctcatga acttccagcc agttcgagcc atctggttca gtggtgtatt
      301 ttactggctg gtcgataatt tgcacacgcg tcccaacagg aacattatca aacagatatt
      361 tgatatcgtc attgcgcaga cgaatacagc cctgacttac ccggagccca ataccaaaat
      421 tggcattggt accatggatg gcatacaacc tgccaatata aatcgcgtac agccccatgg
      481 gattatcggg gcccgcagga acaaatgcgg gcaaactctc ccctcgtttc gcatattcgc
      541 gccgagtgtt cggcgttggc gtccaggttg gagcttcttg tttacgttca acggtagtca
      601 cccagttacg cggggtttct cgcccagcct ggccgatacc aataggaaag acttccacag
      661 tattactgtc tggtgggtag taataaagac gcatctcagc gacgttaacc acaatccctt
      721 tacgaacagt ggcgggcaaa atcagttgct gcggaatggt gagttgcgag ccagacttcg
      781 gcaaaaaaaa catcagcgcc cgggttcgct tccagcatgt tacttaaccc ttgcccgtat
      841 tgtgcggcaa aagtctccag cggctgggta ttgtgatcag gaacagttac agtaagcgac
      901 tgccccacta aacggctacc ctctggaggt aatggataag ttaccgccag gctattatgg
      961 ctggcaaaaa gcagagcaaa tgagcaaaaa atatttacac gacgcatcat gtccctttcc
     1021 taagtcgcga aagctatccg ttaagtatag cttttatcag acttttcgtt tttaactgtt
     1081 caaatcagaa gtcgtattcc ccggtagaac aatattactg gcagcaagtt cgcccatgtt
     1141 gttatatatc gcacaggcag cttcgatgat gggcatcgcc agagctgcgc cactaccctc
     1201 acctaaacgc atctccatat tgagataagg ctccagcccc aaatgcgaca gcgcaatacg
     1261 cgcccctttt tctgccgaca ggtgagaagg aatgagatac ggtttgattg caggagacat
     1321 ctggcaggct gcgagcgcgg cagcataaga aagaaatcca tccagcaaca cgggtaaacc
     1381 gcaggaagca gcgcctaaca tcacgccagc cattccgacc aaatcaaatc cacccacttt
     1441 tgccaggaca tcaacgccat cctgcggatt tggttgattc aacgtaatcg cccggcgcac
     1501 aacatcaatt ttattagcca gtttatctgt cggcaggttt gcgccaatcc caaccacttc
     1561 ttcaggagcc cggccagtga ttgtgctgac tattgccgct gccggtgtcg tgtttgccat
     1621 ccccaggtca cctacaccaa acagcgtaac accgtttttt gccagctccc gcgtataaca
     1681 tatgacgtcc aaaagcaact tttctgccag atggcgactc attgccggag ctgaagcaat
     1741 attgccacta cctcgagcaa cacgcatgtt gataagcccg gggataggct cagcagtatc
     1801 aataccaaca tcaattacgt agacgttagc gcccgcttgt gctgccagca cacacacgcc
     1861 ggttgttccg cgggtcatat tttcagcctg aatggctgtc acttcttttg gggaaatagc
     1921 gaccccttcc tcccagacgc cgtgatcggc acacatgacc agtaccgctt ttttgcccac
     1981 atgcggaacg ccattcaacc ccggcattcc tgccagttgt atggcaagcg cctccagcct
     2041 tcccaggcta ccaacaggtt tgagtaaccc gtcgatatgc cgttgtgcac gcgacatagc
     2101 ggcaggatca atagcaggga tcgtattcag taaatcggca agtgtttgca tgtcacgtct
     2161 ctttatagca gtgccagcag gaagaccagt tcaccaagct ctatagcagc tcccagcgta
     2221 tcgccggttt gcccgcccag cgtacgtttg agaagctgac cgaggatgaa aatcgccacc
     2281 atcgtcacca ccatagcagc cacaccatgc atacccggca ataatacggc agcaaaaata
     2341 gcggctaagc cgagtgtgac gcaggtttgt cgcccatcaa ttttgccaat gaacacattg
     2401 ccaagtcctt cttcccgggc ataacgatga cgatacatca ataatgcggc agtcccgcgg
     2461 ctgaccgcac atgctgccgc cagcgatgca aggatcggtt cgccacgcag agccaactcg
     2521 cttaatacca gaatctttgc cagtacaaca aaaatcaatg ccagaccgcc gtgggtgcct
     2581 aaacggctat cacgcatgat ttccaacatg cgatcgcggc tacgtgcaga aaatacgcca
     2641 tcacaggtat cggcgaggcc gtccaggtgg aatcccccgg tcatcagcgc cagcacaagt
     2701 acgctaaaca gtgccgccag tggtacgcca caccatgcct gcagcaccat gaagaccagc
     2761 ccgctaatcg cgccaagcaa taatccaatt aaaggaaaag taataatacc gcgagaataa
     2821 tgctcgaaat ccagtccctg ggaccagcga cgcggtacag gcagacgcgt aataaaagag
     2881 agcatcgccc aaaataattt actcatttga tttttactcc aatacccgaa accaccaacc
     2941 atacttcatt tgccgcagcg gccagctgct gatttacccg cccggcaata tcacgaaaat
     3001 gtcgtgccag acgactctcc ggcacaatcc ccattcccac ttcgttagtc actaatacaa
     3061 cctttgcggg acaacgttgg caggcagcaa tcaacgactg aatctcagca ttaatcgcct
     3121 gttccatcgc ctgataatcc cattcatcag ggtctttatc gccgccataa tcaaacaaca
     3181 gattagtcac catggtggta acgcattcca gcaatacagc ctcatgcggg ttaatgtctg
     3241 catgaattaa ttcatcaaga tgttgccagc gctccactgt gtgccagtgc gccgggcggc
     3301 cttgccgatg atgttctatc cgtgcagcca tctcatcatc aaggatttgt gaggtggcga
     3361 tatacagaac ctgcgaagag tccccaataa gcgcctctgc atggcgactc ttcccgctcc
     3421 gtgcgccccc cgtcaccaaa atcatcatac cgactcctga ttcaattgta tgatggtatt
     3481 cattgaacag aacaatggat cccccaccgt tgctgaccgc aaaacattta cgcgattaat
     3541 actgcgcgta atataattaa aaatccagca ttctcaatcc attacgaaag aagatggcat
     3601 tgcggaatag ccagacttcc tcgtgttaac gtccgacaaa atgcgtcatt gacagcggtt
     3661 tctctggaat gacagatttc attcagataa tggattaatg ctacagcccc accagccgga
     3721 ccagtcagca aacgggcatt gtgttctgca atcaaaatta ccgtcgttcc ctcagaatga
     3781 gcgagccatt ctggtacgga tatctcgctt tcagctgcta tcaatagcag cactgaataa
     3841 tcgctatacg catctgataa cgattgcaca ggcccaccat caagaggctg gaaatcttgc
     3901 tgccagtcct caccaacaaa acaggttatt ggcaatattt cagccagatg tttcgccaga
     3961 tccacatctc cagataagag gcaaaaaaga gctatcgatt cgggttttat cgttttcatg
     4021 cctctcgccc tccacttaat ttcctgtaat ggcggtctgg ttgataagcc atgaaacaat
     4081 attgccacac cttgaatgca gccctggtgc ctgtcggcac tgcgatgaaa gaactaaatg
     4141 atgccgccat accctgatcc tttcagggtg gtaggggtat aaagacgcgc cagtactcta
     4201 ccggacgctt ccgggcaacc cacttcccac cgaagttgtt ggtattgact gacagactgg
     4261 tcttctggct tagcgtcatc ctcacccgac cttcccaatc tcgcgatcag tggcattttc
     4321 gggtttgtca gcattacagc agcgggggct gcgagggatt ttcaccctct tcccaggcac
     4381 aaatgtgcca cacctgtcag cgtatgttat gagctgattt cacttttccc ataacacgta
     4441 aataacgtta attattcatt ccagcgttta tttaaacctt cccttttttt agcatttatt
     4501 ctataccgtt tccatcgccg cattttcact cacttgctgt cgatacacct gcggcgtaac
     4561 ctgataagtc tgacggaaaa ctttgcaaaa ataactgtat tgtcgtgggg attaggcgca
     4621 cgagtctggc gcaccgaatc tgaccgagat ttgttctctt taataaccac aaaattgagt
     4681 tgttttttga ggcaattttc cacaatcaac ttcaacaaat cagcagaggc cataactctt
     4741 gagtaatcca tctcaggaac tttgcggaat tcattaagta attcgggatc tgcctgccag
     4801 caatcatcga cattcagaat atcgaccaaa tcgacatcat cattacgcag acgaacttgc
     4861 ccgcacaata caaaaccgac taaatgtccg gcgattacca gtggaataga gaaatcggtt
     4921 aatccggcat gacagcgata aatacaaggc tgatcggatt tagaggcttc caggccccca
     4981 caacgatcgc tcatgcgaca tcgagtgctt agctgagggt gttgccgcat catctgacaa
     5041 aatggagtga agttaaacag ctcggaaatt tcatcgccgt gaatattgac cacgacaacc
     5101 gcaaggctgg tcgcctgcgc aaaatcctgc gcgattttat taatgagttc tgaattaagt
     5161 gtcgccgcag aaatcatgat aaaacctccc agttaattta ttgttatagc aaaacaattg
     5221 ttttacattt aatcttcaca tcatccctga tgatttactg acgagaagca caaaggacaa
     5281 taacaaaagc ctccgtgaag cgggagactt tttaataagt tcggaaagca gatcacaaac
     5341 agtaaaaaaa taaatgttta taactgccag agtatttcgt tattcgctaa ttcctttagg
     5401 tgtaacacta ttgacaggta aattctttgc aatgagatag cgataaatcg ccgcaccaac
     5461 acatgcccca atgatcggtg caataatggg tacaatgaaa taaggaatat tgcgtccacc
     5521 agtcatcgca atttctcccc accccgcaag ccaggtgaaa atcttaggtc caaaatcacg
     5581 cgctggattc atggcaaaac cggttaatgg tccggtagaa gcaccaataa cagcaaccag
     5641 aataccaata agtaaaggtg caagcggtcc tttgggtacg ccattaccgt catcggtcaa
     5701 cgccataatc aaccccatta acatggacgt tatgaccact tcgaccaacg cagcttgcca
     5761 tacattaatc gccgctgccg gataagtact gaagatactg gcgagttgta aactttctgc
     5821 gctaccacgc accatattgt gagcagattc aaaatcggta aacagactac tgtatagaga
     5881 atatgccaga actgcaccac caaaggcacc cgctaattgc gccagacaaa atggcagcac
     5941 tttacgggct gggaaagagg caaacagcca cagagcaatg gtaactgccg ggttgagatg
     6001 agcgccggaa atacctgccg taaggtaaac agcaagcgaa atccctaacc cccaaatgat
     6061 acaaatttcc cacagtccca gacttgcccc ggcaactttc agggcgcaca aacaaccaat
     6121 gccaaaaaag aggaacagtc cggtgcccaa aaactcggca acgcattgcg ccttgagtga
     6181 atcattcata gtgacacctt ttttacggag tttttcttta tcagccaact atgcgctgac
     6241 tgttgtatat accgagcaca actataaaaa attgctctgc agaaaatatt gccaacatgc
     6301 atgacagact cgtgtaaaaa tcgtgctaac ataacaaatt attgtcatta ccgaatcatg
     6361 caacacagcg ttagtcagtg ttgaaagtaa taattcccac caaactttta caataaaaaa
     6421 ttgccatcat gacataataa aattgaaaca aatatcacag ttccttcttc gaactaaact
     6481 caaccaaata ttactcgcga ttaaactcac tagccaacgc aattaattca cattgaaata
     6541 tccacaaaac aaccaataaa ttaacaacat atagttcata aaagcaattt tataatattt
     6601 gttctctatt aatttctctc attagccgat taagtcagcc attctttttc ctcgcttttt
     6661 tttatagtca tatcgtcaaa aaaatccagg cgaggtcttt atgcaacaag aagcgttagg
     6721 aatggtagaa accaaaggct taactgccgc catagaggcc gcagacgcca tggtgaagtc
     6781 ggcaaatgtt ttgctggtcg gctacgaaaa aataggttcc gggctggtga ccgtcatcgt
     6841 tcgtggtgat gtcggggcgg taaaagcggc aacagacgcg ggtgccgcag ctgcgcataa
     6901 cgtcggcgaa gtaaaagccg tacacgttat tccccgccca cataccgatg tcgaaaaaat
     6961 cttaccaaag ggaattaacc catgagcagc aatgaactgg tcgaacagat catggcgcag
     7021 gtcatcgctc gcgtagcgac gccagagcat gaggttctcc cgaaaaccac tcatcaaata
     7081 cgagagacgg ctatggcaga aaaaagctgc acgctaacag aatttgttgg caccgctatt
     7141 ggcgacactg taggtctggt cattgcgaac gttgatagcg ccctgttgga cgcgatgaaa
     7201 cttgaaaagc gctatcgttc cattggcatt ctgggtgcgc gtattggcgc agggccacac
     7261 attatggccg ctgatgaagc ggtaaaagcc accaacactg aagttattag cattgaactt
     7321 cctcgcgata ctaaaggcgg tgccggacac ggctcactga tcctcttagg tggcaacgat
     7381 gtttccgacg tgaaacgcgg tattgaagtc gccctgaaag aactcgatcg gacctttggc
     7441 gatgtctatg ccaacgaagc cggacatctt gaactgcaat ataccgcccg cgccagctat
     7501 gcactggaaa aagcgtttgg tgcaccactg ggacgggctt gcggcgttat tgtcggcgct
     7561 ccggctgctg tgggtgttct gatggccgac accgcgctga agtccgccaa tgtggatgtt
     7621 gtggcataca gctccccagc tcaagacacc agctacagca atgaagtcat tctgattatc
     7681 tcgggtgact ccggcgcagt ccgccaggcg gtcatctccg cacgcgaaat cggtaaaacc
     7741 gtgctttcga ctctcggcga tacaccgaaa aacgatcacc cttcctacat ctgaattctc
     7801 tgaggcagat ttatgagatc gaaaagattt gaagcactgg caaaacgccc ggtcaaccag
     7861 gacggtttcg ttaaagagtg gattgaagaa ggctttattg cgatggaaag cccgaacgat
     7921 cctaaaccgt caatcaaaat tgttaacggc gtcgttaccg aactggatgg aaaacccgcc
     7981 agccagtttg acctgatcga ccactttatc gcccgctacg gcatcaatct cgcgcgcgcc
     8041 gaagaagtca tcgcgatgga ttcggtaaaa cttgccaata tgttgtgcga tccaaatgtc
     8101 aaacgtagcg atatcgttcc ccttaccaca gcaatgacgc ctgcaaaaat cgtcgaagtg
     8161 gtttcgcaga tgaacgtggt agagatgatg atggcgatgc agaaaatgcg cgcccgtcga
     8221 acaccatcac aacaggctca cgtcaccaac gtcaaagata acccagtaca aattgccgcc
     8281 gacgcagctg aaggcgcatg gcgcgggttt gatgaacagg aaaccaccgt tgccgttgct
     8341 cgctatgcac cgttcaacgc catcgccctg ctggtcggct cacaggtggg acgtccgggc
     8401 gtcttgaccc agtgttcact ggaagaagcc accgaactga aactcggtat gttaggccac
     8461 acctgttacg ccgaaacaat ttctgtctat ggtacagaac ctgttttcac caatggtgat
     8521 gataccccgt ggtcaaaagg gtttctcgcg tcatcctatg cttctcgtgg cttaaaaatg
     8581 cgctttactt ccggctccgg ctctgaagta cagatgggtt acgcggaagg caaatcaatg
     8641 ctttacctcg aggctcgttg catttacatc accaaagccg caggcgttca gggtctgcag
     8701 aatggttcgg taagctgcat cggtgttcca tcagctgtgc catcaggcat ccgcgcagta
     8761 ttagcggaaa acttaatttg ctcatcgctg gatctggaat gcgcctccag taatgaccag
     8821 acctttaccc actcggatat gcgccgtacc gcacgtttcc tgatgcaatt tctgccaggc
     8881 accgacttta tctcttccgg tttttccgct gtgccgaact acgacaacat gtttgctggt
     8941 tcaaacgaag atgctgaaga cttcgatgat tacaacgtca ttcagcgtga cctgaaggtt
     9001 gatggtgggc tgcgaccagt acgcgaagaa gatgttatcg ctatccgcaa taaagccgct
     9061 cgcgctttac aggctgtctt tgccggtatg ggactaccgc ctattaccga tgaagaagtc
     9121 gaagcggcaa cctatgccca cggttcaaaa gatatgcctg agcgcaatat tgtggaagac
     9181 atcaagttcg cccaggagat tatcaataaa aaccggaaca gcctggaagt ggtgaaagcc
     9241 ttggcgcaag gcagttttac cgatgtcgcc caggacatgc tcaacatgca aaaagcaaaa
     9301 ctgaccggcg attatctcca tacctcagcc atcatcgtcg atgacggaca agtgctctct
     9361 gcggtcaatg acgtcaatga ttacgccgga ccggctacag gttaccgcct gcaaggggaa
     9421 cgctgggaag agatcaaaaa tattcccggt gcacttgatc ccaacgaaat tgactaaggg
     9481 tgagaaatgg aaattaacga aaaattactg cgtcagatta tcgaagacgt gctggcagaa
     9541 atgcaaccca gcgacaagtc cgtttcattt cgcgcacctg tctctgcaac tgtcccttcc
     9601 gcgccagata ccggaaattt tctgaccgaa attggcgaag cccaacaagg cactcagcaa
     9661 gatgaagtca ttattgctgt aggcccggcg ttcggcctgg cgcaaaccgt caatatcatc
     9721 ggtattccgc ataaaaacat cttgcgcgaa gtgattgccg gtatcgaaga agaaggcatt
     9781 aaagcccgtg taattcgctg ctttaaatcg tccgatgtcg cattcgtagc agtcgaaggc
     9841 gatcgtctta gtggttcagg tattgctatc ggtattcagt cgaaaggcac aacggttatc
     9901 caccagcagg gacttcctcc cttgtctaac ctggaacttt tcccacaagc gcctttgttg
     9961 acgctggata cttatcgtca gattggcaaa aacgccgcgc gttatgcgaa gcgcgagtct
    10021 ccacaaccgg tgccgaccct caacgatcaa atggcgcggc cgaaatacca ggccaaatct
    10081 gccattttgc atattaaaga gacgaagtac gtggtcactg gcaaaaaacc gcaagaactg
    10141 cgcgtgacgt tttaacaaag gaaaccttca tgaataccga tgcaattgag tcgatggtca
    10201 gggacgtgtt gaaccgcatg aacagtttac aggatgcagc tcctgtttct gcggtcccca
    10261 acgcatcaat actgagcgcc aaagtgacgg attacccgct ggcaaataaa catccggaat
    10321 gggttaaaac agcgaccaat aaaacgctcg acgatttcac tctggaaaat gtgctaagcg
    10381 ataacgtcac cgccctggat atgcgcatta cccctgaaac attgcgtatt caggctgcga
    10441 ttgccagaga tgctggtcgc gatcgtctgg cgatgaattt tgaacgcgcc gctgagctta
    10501 cctcagtacc agatgatcgc atcctcgaaa tctacaatgc gttacgccct tatcgttcaa
    10561 ctaagcaaga acttattgcc attgctgacg atcttgaaca gcgttatcag gccaaaattt
    10621 gtgctgcatt tgtccgtgaa gctgcggaac tttatgtcga gcgcaaaaaa ctgaaaggcg
    10681 acgattaagg acgaactatg cgatatatag caggcattga tatcggcaac tcatcaacag
    10741 aggttgcgct ggcaacgctc agtgcaactg gagagttgtc gttcgtgagt agcgccctgg
    10801 aggaaaccac cggaatcaaa ggtacgttgc gtaacgtcca tggtattcag gaggcgctcg
    10861 cacaggcaac gaaaaaagtc ggcatcaatg ttagcgatat ttcgcttatt cgtatcaacg
    10921 aagcaactcc ggtcattggt gatgtggcga tggaaaccat cactgaaact atcatcaccg
    10981 aatcaaccat gatcggtcac aacccgaaaa ctccgggcgg agtggggctg ggcgtagggt
    11041 tcaccatcac tccgcaagag ttattaacac gcccggcgga cacaccttac atcctggtgg
    11101 tgtcatcggc atttgatttt gccgatatcg cgacgatgat aaacgcctcc gtacgcgccg
    11161 gatatcagct gactggcgtt attctgcaac gcgacgatgg cgtactggtc aataaccgcc
    11221 tggaaattcc tttacctatt gtggatgaag tcctctacat cgatcgtatt ccgctcggca
    11281 tgctggcagc aattgaagtt gccgttcccg ggaaagttat cgaaaccctg tctaacccgt
    11341 atgggattgc caccgtattc gccctcaatg ctgaagagac taaaaatatt gttcctgtcg
    11401 cgcgggcgct aatcggcaac cgttcagcag tggtagtgaa aacgccctct ggcgacgtta
    11461 aagcccgttc gatacccgcc ggaaatatcg aactgctttc tgccgggcgc acaacgcgcg
    11521 ttgatgtagc tgctggtgcc gatgccatta tgaaagcggt cggtgagtgc ccaaaactgg
    11581 aaaacgtgac gggagaacca ggcaccaaca tcggcggaat gctggaacat gttcgccaga
    11641 ccatggcgga actgaccaac aaacccagca acgaaatttt tattcaggat ttgctggcga
    11701 tagatacctc tgtccccgtc agcgtaaccg gtggtctggc aggtgaattt tcactggaac
    11761 aggctgtggg tattgcctct atggtgaaat ccgaccgtct gcaaatggcg atgatcgcca
    11821 gtgaaattaa gcaaaaactg catgttgatg ttcaggtcgg aggcgcagaa gcagaagccg
    11881 ccattcaggg ggccttgact acccctggaa ccacacgtcc actggcaatt ctggacttag
    11941 gtgcaggctc taccgatgcc tccatcatca atcagagtgg ggaaatcgtt gccactcacc
    12001 ttgcgggagc gggcgacatg gtaacgatga ttatcgctcg tgagctgggc ttaaatgatc
    12061 gttatctggc agaagaaatc aaaaagtacc cgttagcaaa agtagaaagc ctgttccact
    12121 tacgccatga agacggtagc gttcagttct tccctactcc cctatccccc catgtatttg
    12181 cccgcgtctg tgtagtgaaa cccgacgaac tggtgcctat tcccggtgat ttgacgctgg
    12241 aaaaagtacg cgctgttcgt cgcagtgcga aagagcgtgt cttcgtaacc aatgccctgc
    12301 gcgcgctacg tcaggtaagc cctgccggaa atattcgcga cattccgttc gtcgttctgg
    12361 tgggtggttc ttcgctggat ttcgaagtac cgcaattggt caccgacgcg ctggcacatt
    12421 atcgactggt cgccgggcgg gggaatatcc gtggtagcga aggtccgcgc aacgcggtag
    12481 ctactgggct actcatcgcc tggcacaagg agtcgattca tggaaagtaa agagcgtgca
    12541 ccagccatcg ttgtcatgga gatcggtgac tgtatcacta catgggatga ggtgcttctt
    12601 ggcattgaag aggaaggtat tccctttcgc attcaacaca ttccgtcagg tgaagttatc
    12661 gatagcgcct ggctggctgc tcgtcaatct cccctgttgg ttggcattgc ctgcgaccgg
    12721 gaagaactga ttgtgcatta caaaaactta ccggcatcag cgccactgtt tacgctgacg
    12781 tatcaacaag ataaccatac ccggcgcagc atcggcacta acgctgcccg cctggtaaaa
    12841 ggaattccct tccgggaatt ccactcataa tccacaggag aaacgctgta tgaacaacgc
    12901 actaggactg gttgaaacaa aagggttagt tggcgcaatt gaagctgcag atgccatggt
    12961 taaatccgcc aacgtgcagc tggttggcta tgaaaagatt ggttcgggtc tgattacggt
    13021 gatggtccgc ggtgatgttg gcgcagtaaa ggccgccgtt gatgcgggga gcgctgcggc
    13081 aagcgccgtt ggcgaagtga aatcctgcca tgtcatcccg cgcccacaca gtgatgttga
    13141 ggctatttta cctaaatcgg cataacccca tttgctaagg agcacagcgt gaagcaatca
    13201 ctgggattac ttgaagccag tggtctggct ctcgccatca gttgcgcaga cgctatggca
    13261 aaagccgcgt ccatcacgct ggttggcctt gagaagacca atggctcagg ctggacgctc
    13321 atcaaaatca ccggtgacgt cgcctcggtt aatgcggcga tcgcgacggg agccagtttc
    13381 gccggacagc gaaatggcct tgtcgcacac aaagtgattg cccgcccggg agacggcatt
    13441 ctgccccaca caactgaagc attgaccgtg acggttgaaa caagcaaaac accagcaaca
    13501 accgtcacct cagagattat cagttgtaat ttgtgtcttg acccggcatg tcagcggcaa
    13561 aaaggtgagc cccgctcact ttgcctgtac gccaataagc gaggggagta gcagtggata
    13621 aacaaacctt gcaggaaaca gtgagccaga ttctggatga gatgcgcgag cgccctatcc
    13681 cactgggcgt ttccagtcgc catatacatc tttcatcagc ggattatttg cgtctttttc
    13741 ctgaacaacc gattagcgag aaaaagccgt taatgcaacc tggccaatac gctgctgaac
    13801 aggttgttac gctggaggga ccaaaaggca aattgcacaa cgttcgcttg ttggggccgc
    13861 tacgcaatgc aagccaggtg gaaatttccc ggacggacgc cagaacgtta gggatcaccg
    13921 ccccgctacg catgtcaggc gatctcagcg gcactccggg gattcgtttg ataagcccat
    13981 ttgcggaact ggagatcaca tctggcgtca ttgtcgcgca acggcatatt catatgtctc
    14041 cgctggatgc cctgatttat ggcgttacgc atggcgataa agtggcggtt gccattgaag
    14101 gtgatgagcg acgactgata ttcgaaaacg tcgctgttcg cgtctctccg gatatgcgtc
    14161 ttgaaatgca tattgatacc gatgaggcca acgccgcagg cgctgatagc cctgacgttt
    14221 ttgccacact ggtgtcagtg ccatgaataa catgctgatg caacgtattg ttgacgaagt
    14281 ggtttttcgc cttaaacaac gtgcgggaaa aacactcgtg ctgactgttt ttcaattgcg
    14341 agacgccagc gttcaagaga gcgttcatca gtatgcgtca ttacaaatcc gatatgtcga
    14401 tctgccgctg ctgcgacaac ttgcggagaa cgaaacgtca gatcgagcat ctatccagat
    14461 acacgaagcg ttagcctggg gaatgcacat tcaattgtcg ttgcagcgtc atttcctgaa
    14521 cgccatcgaa ttgaagactc tcgcccgtct accgctaagc tggtgcgacg aacaagggca
    14581 gccgatctac cttcatcata atcgactttt aagttatgcc gatatcgcac agttaagctc
    14641 gggaatactg gtgttacagc ggaaatgttg tgtgaccgca ctggctcgtg aagcggcaat
    14701 tacccgaaat attcagttaa tcaggcagga gtaacctatg catctggctc gagtcactgg
    14761 cgcggtagta tccacgcaaa aatcaccttc tctgatcggg aaaaaactgc tgctggttcg
    14821 ccgggtcagt gctgacggcg aactgcctct cacgccatta accggcgacg aagttgccgt
    14881 tgattcagtc ggcgcaggcg ttggtgagtt ggtactcctc agcagtggtt ccagcgccag
    14941 acatgtgttt tcaggcccta acgaggccat cgacctggca gtggtcggca ttgtcgatac
    15001 gctttcgcgc taaggagcta aaagatggca atttataccc gaacaggtga ttcaggaagt
    15061 acgtctttat ttaccggaca gcgcgtcagc aaaactcatc tacgtgtcga aacctatggc
    15121 acgctggatg aactgaacgc gacactcagt ctgtgttatt gcgccaccgc catcgaaagc
    15181 catcgcatct tactggaagc cattcaacaa cagatatttt ggtttagcgc cgaactcgcc
    15241 agtgaaagcg aacagccgtc agcgcaacag cgttacatcg gtacggaaga aattgccgcg
    15301 ttggaaaacg cgatcgatag tgcaatgaac gccgtccccc cagttcacag cttcattctg
    15361 cctggtcgat gtgaagccgc aagccgcatg catttcgccc gcacggtggc tcgacgggct
    15421 gaaagacggc tggttgaact gacaacggaa accaccgtcc ggaatgtttt gctgcactat
    15481 atcaaccgac tttccgattg cctgtatgcc ctcgcccgcg tggaagacaa cgttgctcat
    15541 caaaacctga tgattcagga aatcacaaaa cgttatcacg aagccaacca cacaccagca
    15601 ttgaaggaac gcacaatgcc gctcactttt caggatcttc accagctcat tcgtagtgcg
    15661 gcaatgcgcg cggatgaact gcatattccc gttgtcatca gcattgttga tgccaacgga
    15721 acagaaagtg ttacctggcg gatgcctgat gccctgttgg tcagcagtga actggcaccg
    15781 aaaaaagcct ggaccgccgt ggcgatgaaa acggcaaccc acaagcttgc tgataccgtg
    15841 caacccgacg cgccgcttta cgggctcgaa agccatatgc aaggaaaagt cgtcactttt
    15901 ggtggcggct tccccctctg gcgtgacgga aaattgcttg gcgggcttgg catcagcggc
    15961 ggtagcgttg aacaagacat ggatattgcc caaagcgcaa tggcggcaat taacgtggga
    16021 gtaaaccaat gaataactct gaattagaaa cactgatccg caatattctg cgtgagcaac
    16081 ttgtccccgc tacaccacga acgcaaagaa acgctatttt ccagactgtc gatgaagcgg
    16141 tctgcgcagc acactaggcc tttttacgct ttcaacagtg cccgcttaaa acccgtagcg
    16201 ccattatcag cgccattcgc gaagaattaa tgccccgcct taccgaactg gcagaagaaa
    16261 gcgccaaaga aacggggatg ggaaataaag aagataagta cctaaaaaac aaagcggcac
    16321 tcgataacac gccaggaatt gaagatttaa ccaccacagc actaaccggt gatggtggca
    16381 tggttttgtt tgagtactct ccgtttgggg tgattggctc ggtcacgcca agcaccaacc
    16441 ccacggaaac catcattaac aacagcatca gcatgctggc agcaggtaat agcgtctact
    16501 tcagtccaca cccgggtgcc aaaaacgttt cgttaaaact gattaccaca atcgaagaca
    16561 tcgcgtttcg ctgtagtggc attcataact tgatcgttac cgtatctgaa ccgacctttg
    16621 ctgccaccga acagatgatg gcgcacccta aaatcagcgt tctggcgatt accggcggtc
    16681 cggggatcgt cgccatggga atgaaaagtg gcaagaaagt gattggtgcc ggagcgggta
    16741 accctccgtg catcgtcgat gaaacggcgg atattgtgaa agccgccgag gacatcatca
    16801 acggcgcttc atttgattac aacctgccgt gtattgctga aaaaagcctg attgtcgtgg
    16861 catcagtggc agattattta attcagcaga tgcaaagttt tggcgcactt cttcttaact
    16921 atgagcaaac tgagaaattg cgtgcaattt gcctgccaga cggtagcgcc aacaaaaaac
    16981 tggttgggaa aagcccatca gccttgctgg aagcggcagg cctccctttg cccgcaaaag
    17041 cgccgcgcct actgattgct gttgtcgacg ccgatgatag ttgggttact tgtgagcaac
    17101 taatgccaat gctgcccatt gtgaaggtca aggattttga cagcgctcta accctggcgc
    17161 tgaaagtgga agacggcttg caccacacgg caattatgca ctctcagaac gtctcacgac
    17221 tcaaccttgc cgcgcgcgtt atgcaaacct caatatttgt caaaaacggc ccctcctggg
    17281 cgggtattgg cgtcggcggc gaaggcttta cgacctttac cattgcaaca ccgactggag
    17341 agggtacaac gtcagcacgg acttttgccc gctcacgacg ctgcgtgtta accagcggct
    17401 tttcaattcg ctagagaaac ggaaatgaat actttttcac tgcagacgcg tttatacagc
    17461 ggaccaggaa gtctcaaggt acttagccgt ttctctcata gacacatctg gattatttgc
    17521 gatgcctttc tggcacgctc accacttatt gacacgttgc atcaggcgct gcccgacagt
    17581 aatcgtctca gtatttttag cgaaataacg cccgccccaa ctatccagac cgtggtgaaa
    17641 ggcattgcgc aaatgcagac tttacgcccg gatgtagtta tcggttttgg cggcggttct
    17701 gccctggacg ccgcaaaagc aattgtctgg ttcggtcgcc agtgtggcat tgaaattgaa
    17761 acctgcgtag ccattcctac caccagcggt atcggttccg aagtgaccag tgcttgtgtc
    17821 atcagcgatc ctgagaaagg gattaaatat ccccttttcg ataatgcact ttatccggat
    17881 atcgcgatcc ttgatccatc tttggtcgtc agcgtgccac cggcgataac ggccaatacc
    17941 gggatggatg ttttaaccca tgcgcttgaa gcttatgttt ctcctcgcgc cagcgacttc
    18001 acggatgcac tggtcgaaaa agccgtgcaa attgtttttc aatacctgcc cactgcagtg
    18061 aaaaaaggcg attgtcttgc cacccgtggc aaaatgcata atgcatcgac gcttgcagga
    18121 atggccttta gccaggccgg actcggttta aaccatgcaa ttgctcatca actcggcgga
    18181 cagtttcatc tgccgcatgg actggcgaat gcgctcctgc tcacggcagt tatccgcttt
    18241 aatgctggcg atccgcgcac agccaaacgc tatgcccggc tggcgaaaac gtgccattta
    18301 tgcccggaca acgccaatga caccgccagc ctgaatgcac ttattcaaca tattgagcag
    18361 cttaatacaa cctgtacgct gccgactctt gccaatgcgc tgaaagaaaa aaaagcagaa
    18421 tggtcaatac gcataccaga tatggttcag gcggcgctgg cggatgcaac gttgcgtacc
    18481 aacccgcgtg cagctgatgc ctccgcgatt gcagaactgc tcaaggggtt gttatgaacg
    18541 tcggattaat ctcctgcgac gaccagacca tccgcgagcg agtacaagca gcaggtgtcg
    18601 tgggtgctgg cggcgcaggt tttccaaccc atattaagct acaagcgcgt gctgacactt
    18661 tcctggtgaa tgcggcggaa tgcgaaccca tgttgaaagt agatcaacag ttaatggcgc
    18721 agcaagccag ccgcctgctt cgcggtgttc actatgcgat gaaagccacc ggggccagta
    18781 gcggaatcat cgcgctgaaa gaaaaatatc aacgggcaat aaacgcactc acaccactgt
    18841 taccaccgga tatccgcctg catattttgc ccgacgttta ccccgcagga gacgaagtgc
    18901 tgaccatctg gatggcaact ggtcgccgcg ttccacccgc tgcattaccc gttagcgtcg
    18961 gtgtggtggt caataatgtt caaaccgtac ttaatattgc cagagccgtc gagcaacagt
    19021 atccagtgac gcgccgcaca ctcaccgtta atggtgcagt ggcgaagcca ataacggtaa
    19081 ccgtccccat cggcatgtct ttacgtgaag tcctggcgct ggcgggcggc gcaacagtgg
    19141 atgctcctgg ttttatcaat ggcgggccga tgatgggaag tttgatcaca tcgctcgaca
    19201 caccagtcag caaaaccact ggcggcttgc tggttttacc caactctcat tcactgatcc
    19261 aacgtcgaat gcagaatgac cgcagcgtac ttgccgtcgc gaaaactgtt tgtgagcaat
    19321 gccgcttgtg taccgatctc tgcccacgtc atttaatcgg tcacgaactg gcaccccatt
    19381 tattagtacg tgcgcggtta actaccaaca gctcgccacg ccgcaactgt tactgacagc
    19441 gctcacctgt tcagagtgca acgtctgcgc cagtgtcgcc tgtccggtag gaatttcacc
    19501 aatgcgcata aaccgcctgc ttaagcagga attacgcgca caaaatctcc gctacgaagg
    19561 tgccttaaat cctgcagatc ccatggcaaa ttatcgactg attcccgtaa aacggctggt
    19621 aaccagactt ggcttaaccc cgtggtatca ggatgcgcca ttgtctgaac aggtgcctca
    19681 gcccgaaaaa gtgacgttat tgttacgcca gcacatcggt gccagcgcaa ttgcgtgtgt
    19741 acaaaaaggc gatcgcgtcg ttcatgggca atgtgtaggt caaattccac acggcacatt
    19801 aggtgccccc attcatgcca gtattgatgg catggtcagc gatgtgacag aaaacgcgat
    19861 tacgcttgtg agaggttaac catgtcgaaa gcggtaggta ttttagaact tagcagtatc
    19921 gcgaaaggga tggaaaccgg cgacgccatg ctgaaaagcg ccaacgttga gttactggtc
    19981 agcaaaacgc tatgccccgg aaaatttctg ctgatgctcg gtggcgatgt gggcgcggtg
    20041 caacaggcca tcaacagcgg agcgtccctt gctggtgaaa tgctggtcga cagtcttgtc
    20101 ctgccgaata ttcatcccag cctcctttct gcaatcagcg gacttaacca tgttgaacag
    20161 caccgggctg tcggcgtagt cgaaacctgg agtgtcgccg cctgcattga cgctgccgat
    20221 tgtgctgtca aagctgcaaa tgtcattctt gttcgcgtac atatggcttt tggcattggc
    20281 ggtaaatgct acatggtggt tgctggcgat atttcagatg ttaataacgc ggtctctgcc
    20341 gccagcgtgc gcgcggggga aaaaggtttg ttggtctatc ggtcggtgat tgcccgtcca
    20401 catgaagcca tgtggcgtca gatggtggag ggataatgac aactcagcac acaactgaac
    20461 ggatgattca ggaatatgtc ccaggtaagc aggtcacgct ggcacatgtc atcgccaatc
    20521 cagggaagga tctgtttaag aaattgggtt taccagacac catttccgct attggtattc
    20581 tgacaattac ccccagcgaa gcatctatca tcgcatgcga tatcgccaca aaatcaggcg
    20641 ctgtagagat cggttttctt gatcgtttta ccggtgccgt agtgctgact ggtgatgttt
    20701 ctgcgatcga atatgcactt agccaggtta cccgtacgct gggtgaaatg atgcgcttta
    20761 ccgcctgccc cattacgcgg acctgacgtt atgcaacgta tgatgctcat cggccccagc
    20821 cagtgcggaa aaacgtcact cacgcaatgt ctgcgtggcg aaaatattca ttatcagaaa
    20881 acgcaggcaa tcgtctggtc cccgaccacc attgatacac caggcgaata cctcgaaaac
    20941 cgcagcctct acagtgcact gttggtcagt gcctgcgaag cggacattat cgcgctggtg
    21001 cttaatgcgg acgcaccgtg gtcacctttt tcgccaggct ttacctcgtc aatgaatcgc
    21061 ccggtcattg gagtagtaac caaagcagat ttagccaaca tggaacaaat atctttggtg
    21121 aaatgttggt tacgggaagc tggagcgcac aacgtgttag taaccagtgc ggttaataac
    21181 catggagttg cagaactttt agccttgctg catacagaag aaggctgtcg ctaatttcga
    21241 caggttatca gatgggaatg atgacggtga tacacagaaa agaatcaatg agcaatgagg
    21301 gttgccgggc aaccctcatt gaataaaaca ggaattactt cgcttcgccg tttttcattt
    21361 cgcccatagc tttcagcttt ttggagatat cgcggcgttc tttggagagc tcggcatttt
    21421 tgatgatgta atcgtcaacg cgatcttcat agtcaccttt catgctggcg atgatgccct
    21481 ggattgcttc aacgctcatc ccaggcttga tgtaatcgct caggttgtcc agcagcagga
    21541 cgcgcttctg gttgtcacgg atctttttct caacgtcctg aatttcacgt tgcagtttgt
    21601 tcttacgacg gaacagacga acaaattcca gtacgtcctg aaatgaaggc ttggtagttt
    21661 ccatttttat acccctgata atgtgagagt cggattcgtt taagcaaccg ctattcgtta
    21721 gggccaacat tactcatagc tgccgcagat gacaatgctt ttatcccttt tcactatcat
    21781 accctttatc cttgctgaat cgaagcagca gcaagatgat tctgaagttc aggaattatt
    21841 tgcgcaaggc ccggcaaatc agattcgata gcaactcaag ctgatgcgcc gtttccatgt
    21901 tcagccacac ataaccatag attggtgttt ccactacctt caggtcatgg tggttattga
    21961 gcaactgacg cagctcttcc acagcatcgt tcaatttttc cgtattggca aaaaccggct
    22021 gcggattacc ttcgtacagc gcatgaacaa agctcagcag tatttgctgc atcatgtgct
    22081 gggtatcacg aagtttttgc gcgttcaata acacgaaatg gctggggcgc gtggcccagt
    22141 acgcattgat ttgcaactcc agcatacaaa ccagattgcg gttaatggtc tggatacctt
    22201 catatatcga ttttggaata cgggtttctt tgctggcggg cgcaatcagc ccacgcattt
    22261 tcacggcatc ggtcaggagt ttttgtagat ggctttccag acgtgggcgt tcgagtaagt
    22321 tcggtgagaa tgcagattga tagacccgat tatactcggt cagacttttc gccagttgaa
    22381 tgcgccagtg gatgaacgcc cgttgtggcc agataccggt aaacaacatt gccagtaaag
    22441 agccgaggat cacatcgccg cttcgccata acgccgtatc aatttcacct gtcggggaac
    22501 caacaacaat tgccagcgtc accccaatca ataatccttg atacggtttc ttgcccagcg
    22561 ccagccaacc gcaaagaaac atcgccgccg cgcaccagac taacatcagc ggtaacgaaa
    22621 ttaactccag ttgcagagcg ataagaccta aaatcgaacc caacaccgta ccgccaatac
    22681 gttcgaaggc acgagggaca acgttacccc agaacgagat tggccccata atcaccacca
    22741 tggtgaccag cggccaggtg ctttccggga tagtaaacag gcggataatg agaaaagtga
    22801 gcaggaatgc cagcgcgacc cgagtaccat gcactatgcg gtaatggcgg tatacccgga
    22861 tttcaaacgg gcttaatgac ttatcggcac gcacgcagaa actccgtaat gaagattaaa
    22921 agcgctcata actcatctgt cggccagaag gcgcgcctct gaattatccg acggacaaac
    22981 cgggaacagt gtaatttccc ggcaggagcg aaaaaattga aaggcgcatc ttactctttt
    23041 cgctttcaaa aaaagagtgg tcatcgcgtt aacacaccgc cctgagatga attagtgatg
    23101 ttttggctgc accgcaacat acatttcgat atcccagtac ccatcttccg cgccattgtt
    23161 caaataaacc tcgaagcagg gctttggtaa catttcataa gcactgtcct gcaagaggct
    23221 attaaagaac tgataccagg gtttagcaaa atcatcaccg actacacgag ccaccgccac
    23281 cgcatactga ccacctgaaa tttctgtcag aatgacgccc tcactgtttt cgggaagcgt
    23341 aaagttattc ggtaccgtca caacggtgtc gcagcgtaat ttttcggcgg gtgtttcatc
    23401 tggattgtcg taatagacag caacccactc cttcggcaca atatttttgt tatctaccca
    23461 catcatcaac tgctcaaagc ctttctttac cgtctgttcc cacgggccaa cgagatggaa
    23521 acctgcaacg gtacgtttct cttcctgcgt aatctcgtag ttcatgacgc ctccattaac
    23581 actgttttta tatacagcat agttgcaata ttaaaaccac aaggaatgag tgttgattat
    23641 gcgagcagac tcgcactcct gccagtctgc tgcaaaagaa aggtcaggcc ttatggtgga
    23701 aataatcact caggcgggaa aacatgccgc cttccccgac agattccagg gtaaccagcg
    23761 gccagtgcgc cacctgttta tcacggtcgt aaagttcaat ttcccctacc cgctgatggg
    23821 cgctaattgg cgcggtgagc tctttaccat caagggtata tttggctttg atatgtggaa
    23881 tttcggcttt cggtagcacc atccagaact cttgttccgt tcctaggtcg atattttctt
    23941 tatcgccata ccagatgcgt tccgttccga cctttttccc acggtgcaaa atttgcaccg
    24001 tagtaaagtt ttgttgcccc caacgcagta attttcttgc ctcttcctca cgaccttttg
    24061 cactgtcagc ccccattacc actgcaatga gacgacgctg cccatctaca gccgaagcaa
    24121 tgagattaaa cccggcacca gaagtatgac ccgttttcag gccgtcaaca ttcatggttt
    24181 tatcccacaa caacccgtta cggttttgct gggtgatacc gttccaggtg agacttttct
    24241 cactgtacat atgataaaac tcgggctcgc cgtggatgat agcgcgagaa aacacagcta
    24301 aatcataagc cgagctatgc tggccaggtg catccagacc atgcactgtt tcaaaatgcg
    24361 tatccttgag atgcagcttc tcggcatagt tgttcatcat ttcaacaaac tgccgttgcc
    24421 caccggcaat atagtcagcc agagcaacac aagcgtcatt tccggaatcc acaattaaac
    24481 cacggcttaa atcacgtacc gatacgcgat cgccctcttt caaaaacatc agtgaagaac
    24541 cgacaaacac cggattatct ttcgcccacg catcgcgccc cacggtgaca atatcgtctg
    24601 gcgtaatgcg atgactatcg atagcgcgat ccacgacata acccgtcatc agctttgtca
    24661 ggctggcggg attgcgctgt tgatgctcat tacccgcggt gaggatctga ccggtggtgt
    24721 aatccatcaa tacccaggac ccggcatgaa tctctggagg ctgaggtgaa aaaggaatgt
    24781 tttccgccgc aaaaccagac gataagttaa aaacgaacaa agaagcagca ataataagac
    24841 ggcgtttcaa cagcaaaccc tcaggagttt caaatagctg ttctttttac ggaaatactt
    24901 atgaactggc tggaataaag tgcaagaaaa tgtgactacc ctctcatttt tatctgacat
    24961 gatctgttgc cactcgctgc caaattgtgg cgctaaagct gattagcacg gtgatatttg
    25021 atactctggc agacagcaga aataacggat ttaacctaat gatgaatgac ggtaagcaac
    25081 aatctacctt tttgtttcac gattacgaaa cctttggcac gcaccccgcg ttagatcgcc
    25141 ctgcacagtt cgcagccatt cgcaccgata acgaattcaa tgtcatcggc gaacccgaag
    25201 tcttttactg caagcccgct gatgactatt taccccagcc aggagccgta ttaattaccg
    25261 gtattacccc gcaggaagca cgggcgaaag gagaaaacga agccgcgttt gccgcccgta
    25321 ttcactcgct ttttaccgta ccgaagacct gtattctggg ctacaacaat gtgcgtttcg
    25381 acgacgaagt cacacgcaac gttttttatc gtaatttcta cgatccttac gcctggagct
    25441 ggcagcatga taactcgcgc tgggatttgc tggatgttat gcgtgcctgt tatgccctgc
    25501 gcccggaagg aataaactgg cctgaaaatg atgacggtct accgagcttt cgccttgagc
    25561 atttaaccaa agcgaatgga attgaacata gcaacgccca cgatgcgatg gctgatgtgt
    25621 acgccactat tgcgatggcg aaactggtaa aaacgcgtca gccacgtctg tttgattatc
    25681 tctttaccca tcgtaataaa cacaaactga tggcgttgat tgatgttccg cagatgaaac
    25741 ccctggttca cgtttccgga atgtttgggg catggcgcgg caataccagc tgggtggcac
    25801 cgctggcgtg gcatccagaa aatcgcaatg ccgtaattat ggtggatttg gcaggagaca
    25861 tttcgccatt actggaactg gatagcgaca cattgcgcga gcgtttatat actgcaaaag
    25921 ccgatcttgg cgataacgcc gccgttccgg ttaagctggt gcatatcaat aaatgtccgg
    25981 tgctggccca ggcgaatacg ctacgcccgg aagatgccga ccgactggga attaatcgtc
    26041 agcattgcct cgataacctg aaaattctgc gtgaaaatcc gcaagtgcgc gaaaaagtgg
    26101 tggcgatatt cgcggaagcc gaaccgttta cgccttcaga taacgtggat gcacagcttt
    26161 ataacggctt tttcagtgac gcagatcgtg cagcaatgaa aattgtgctg gaaaccgagc
    26221 cgcgtaattt accggcactg gatatcactt ttgtcgataa acggattgaa aagctgttgt
    26281 tcaattatcg ggcacgcaac ttcccgggga cgctggatta tgccgagcag caacgctggc
    26341 tggagcaccg ccgccaggtc ttcacgcccg agtttttaca aggttatgca gatgaattgc
    26401 agatgctggc acaacaatat gccgataaca aagagaaagt ggcgctgtta aaagcgcttt
    26461 ggcagtacgc ggaagagatc gtctaatttg aagcctccgc cgctggtacg gcggaggtca
    26521 atgttgagtc aggctttttg aacggtgata ctccacgctg catcgccaat ctgctgataa
    26581 tcggtgatgg catgcccttc ttctgccgcc cactgtggga ttgcttcagt tgcctgggtg
    26641 caatcaaact caattaccaa ctcatcgccg ctggccattt ctgccagtgc tgcttttgct
    26701 tctatgagtg gaaacggaca gacctgcgtc accacatcca gctttttaat cgccatcttt
    26761 acaccttact taatttgccg cagcagttgc cagtcgcgcc ttacgctgcg gtcgaacata
    26821 cacaagccag gacgcagtcc agactccgag aatcataaat acaaggccaa tccagccctg
    26881 ccaggtcatc attgcagtca taaccagtcc attaccgata aagcaaccac ctgcaatact
    26941 ggcaccgaag cccatcagta caccacctaa cccgctacgt aatgttgttt gtgcatcagc
    27001 tgcgcgaacg cggaactcac ggctcgcttt ggctgcaata aatgacccca cgaagatccc
    27061 taacaccagg aaaacgcccc agttaatgta tttcacgtca cccgcgacca gaaattgcag
    27121 aatattggcc gttggggaag tgattcctaa cccaaacatg cgtccggttg cttcactcag
    27181 gggccaggct aaaagcgcaa tcaaaccgat gagtacagca gtgacaaagg gatgccagcg
    27241 tttttcaaac agaatatgag cgatcccagt tcggcgcggc ggtaaggtcg cgactttgag
    27301 ttttggcttc ttcaactctt tcatcaccac ccagagcgtt atcaccagca aaacggcaac
    27361 caacggccaa acagacaaat taaaagtctc agcaatagag ttatgttcag tactgtagtg
    27421 ctgcaaggtt tgatttaaac cactggcatg tggagaacgc atcaccgcac tcatcaccat
    27481 ataagtgaaa agcgcgatcc aactgccgat caatccctca cccgcgcgat accaggtccc
    27541 ggtggcacat ccacccgcca gaacaattcc cagcccgaag atatacccac ctataacagt
    27601 accaagccac gggaacgcct cggcttcgta agtcagtagg cccgcctgaa ttaacgcaaa
    27661 aacccccacg ctttgtaccg aaattgcaat cagcaaggcg taaaacatgc gattattttt
    27721 cgcgatatac atatcgcgaa aaccacctgt caggcaaaaa cgcccacgct gcatgacaaa
    27781 tcccagcaga gcaccacaaa ttagcccgct taatatcatt gaaaacataa attaattaac
    27841 cagatgaatg ttaatgagga aattattcat gactggtgga atgcagacca ataaccaaat
    27901 tctctaaatt agaacaaatg gttattaatg aggatttcga taaaaaaaaa taaaaaaacc
    27961 ggagccaaat attcattcga ctccggttga ttattagaaa agatgattat gcaacgtctt
    28021 cgtactgcgg aactgggtta cggaagctct tggtgacgca agccaggtaa atcagaccga
    28081 tagcagccca aatcagaccc agaaccattg agctttcttc caggttaacc cacagcgcac
    28141 caacggtcag cgcaccacac atcggcagga acagatactg gaagtgatct ttcagcgtct
    28201 tattacgctt ctcacggatc cagaactgcg agatgaccga caggttaacg aaggtgaacg
    28261 ccaccaacgc accaaagtta atcagcgctg tagccattac caggtcgaag ttgattgcca
    28321 gcagggcaat cgcgccaacc aggatgatgt tcatcgccgg agtacgccat ttcgggtgta
    28381 cataaccgaa gaagcttttc gggaatacgc cgtcacgacc cattacgtac atcagacgcg
    28441 ctacgcctgc atgcgccgcc ataccggacg ccagtacggt aatggtggag aagatcagcg
    28501 cgccaacctg gaacgcttta cccgcaacgt acagcatgat ttcaggctgt gacgcatccg
    28561 gatctttaaa gcgagagata tccgggaagt acagctgcag gaagtaagtt gcaaagatga
    28621 agatcatgcc gccaatcagc gcggtcagga aaatcgcacg cgggatcacg cgctctgcat
    28681 ctttggtttc ttccgacagg ttgctgatgc cgtcaaagcc ggtaaaggaa aagcacagga
    28741 ttgtcgcccc ggtaatcatc ggaataacat gcgcatcgcc agaccagaac ggacgagtgc
    28801 tcgccagcgt accagcgcct tcaccttcaa atacgccata aacaaccatg cccagaatca
    28861 ccgcgatcag cactacctgc aacacgacga ttacagtgtt gaagttcgct acggatttca
    28921 gactacgcag gttaaaggcg gtcataaagg ccaccagcgc caccacaaac atccacgatg
    28981 ggatggaagg caccagagct tcaaaataga ttttcgccag cagaatgttg atcatcggcg
    29041 cgaacagata gtcgagcaga gaagaccaac ccaccataaa gccgacagtc gggctaatgg
    29101 atttctgggc gtaagtatat gcagagccag cagaaggata gcggcgaacc agcttcccgt
    29161 agctcagagc cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc ggcacatgac
    29221 catccgtgag gcctgaaacg ataccaaaag tatcaaacag cgtcatcggc tgcatatagg
    29281 caagacccat cattacaacc ggaactaacg taagcgtttt acgcaattcc acgcgagagg
    29341 tgtttggagt aacgttatgc gacatggtca ttctccttta cggtgataac cgtcgcgtaa
    29401 gcaaaaaatt gccccatttt tttggattcc tcagcgacaa caactgtcga tttttagtaa
    29461 atatctatcc ggtacgaagc ccggcctctt ggtatgaatg attggtttga agcaaaaaat
    29521 aaccgacgct gatgaaacgt cggtttttag tcattttttg acagcggcgc attgtgccta
    29581 aatggggggg aaatgacaag agaatgagaa gcttgtcata ataatttttc tttaaatggc
    29641 tgattttccg tcatcagatt ttgcataaac accggcgatc gcactatttg ctaaaatttc
    29701 atcccgccac cagccgctgg caagccctgc ggtctgttcg ttccagccaa tccagacggg
    29761 ttcaaagaga gtttgggcga ccactttctt ctcgattaac gcgccactat cgagaaaacg
    29821 ttgcaccaga taacggggta aatagccgca gccgaggccg ctaatttgca gctccagctt
    29881 ggttttaaaa tcgaaaacgg taatggcttc ttgttcgtcg agaagttgtg aggccgtaga
    29941 agcgcctgcc tgcgcagtgt cccccaccac aatcgcccga tatcgcttaa tgatacgacg
    30001 gtttaacggc tcttcttcct gggctaacgg atgatgaggc gcgaccgcaa agacttgttc
    30061 cagatcgccc aaccgcgaga aaccaaactc actggaagat ggtggctcat gcatcgcgcc
    30121 aacaataata tccgctcgcc cctgagtcag ggcatcccag gaaccaccga gtacgccatt
    30181 gataaacttc aggcgcgtca cgctatgatg ttgataaaaa gcttcaatca gtggcgcaag
    30241 cagagaaaaa ggaaacgtgt cgtccacgcc aatcaccagc tcgttttccc agccttcatg
    30301 cagtttgatc gcctgctttt ccagttctcg cacggtatgc agaacttccc gccccttctc
    30361 taataacatt tttccggtac gggtgaattt agcgcggtga ccgctacgat caagtaattg
    30421 aatattaagg tcgctttcca gcttgtgaac ggtgtaactc agagcggacg gcgtcttgta
    30481 gagtttagcc gacgccgccg caaaactgcc ttctttttct aaggcatcga ggatcatcaa
    30541 cacatccagc agttgtttca tacacgcccc cttgcagtgc atgcggcgtc ctgctggcgt
    30601 gatcgtttac tccagcggca ttaccagcgg atcgggatac tggtattcaa atcccagttc
    30661 attacaaatc cgactgccat caataatctt acctttgccg ctgtccagac tatttctgaa
    30721 ctgcggaggt tccagcccca gtaaacgggc catctgcgga tagaaaacat tacgcgcagg
    30781 gtgagctggc gcacatatat tatagatgtg tccgccttta ggtgcctgta acaacagagt
    30841 gatagcgcca ataacatctt ctaaatggac taaattaaca ccatgttcac catcaggcgc
    30901 ggtttttccg gcaaagaagc gtccgggatg acgtcccggt ccgaccagcc ccgcaagacg
    30961 cagaatatcg accgaagtac cgggtaaatt gtgcagccag tcttcgagtt cttctaatac
    31021 tcgtccactg ttggtcaccg gattacgcgg ggtggtttct ttcaccgtgc cttgcgcgtc
    31081 gccatagaca gacgttgagc tggtaaaaat aatgcgagga atacgatggg ccagcgcgct
    31141 atccactaac tcttgtaccg cttgtaaata gaactcatcg ccggggccgc tacgacgtgc
    31201 cggaagcgta atgaccagcg catcggcatc catcagggca tccagatcgt cagaatcgca
    31261 aactaactca ggctccatgc gaagcagata gctatcaatg ccactcattc gggccgcttc
    31321 gacaccatct tgtgtggttt tactcccggt gacttgccag cctcgcgctg aaagtgacat
    31381 cgccagcggc atgcctaacc accctaaccc gacaattgcg acctttttca tccgttatct
    31441 cctgatacac cttagatcta taaggctacg ctagcgtatc aaaactgaca attcattcta
    31501 tgaatgaatc tgttcaataa tgataacgac atgctgcaat gagcagtgaa tcgtcggtaa
    31561 ccgcgtatac cagacggtgc tcctctgtaa tgcgtcggga ccagaaacct gacaaattat
    31621 gtttcagggg ttctggcttc cccttacctt caaatggcgt tctgcgggta tctttgataa
    31681 gttcattgat ctttttaaca attcgcttat ctgtttcctg ccagtacaga taatcatccc
    31741 atgattcctc agaccagatt agtttcactc aataatgtcc ttttctgttc ctttgcctga
    31801 tttcaggcta tcgattgagt ccatcaatct ccgggcgtta gcgggggagc gcagtagata
    31861 agccgtctct tccagcgagt tgtattcttc gagtgacatc agaacacaag cctctccatt
    31921 ctgacgagta ataaggatcg gggcatgatc ttcaacggct ttcatcattg ttgccgacaa
    31981 attctgacgc gcttcgctgt agctaattgt acgcatgtca atctcctctt ttgtacagtt
    32041 tattgtacaa tgatgggcgt taattaacta tttattaatt agtttgtaga tcaaggtatt
    32101 gtcagtgaga cgaaaatcca ggcttcgcta tttttggtgc cgtcagctta gaggacagtc
    32161 ctcttagccc cctcctttcc ccgctcattc attaaacaaa tccattgcca taaaatatat
    32221 aaaaaagccc ttgctttcta acgtgaaagt ggtttaggtt aaaagacatc agttgaataa
    32281 acattcacag agacttttat gacacgcgtt caatttaaac accaccatca tcaccatcat
    32341 cctgactagt ctttcaggcg atgtgtgctg gaagacattc agatcttcca gtggtgcatg
    32401 aacgcatgag aaagcccccg gaagatcacc ttccgggggc ttttttattg cgcggttgat
    32461 aacggttcag acaggtttaa agaggaataa caaaatgaca gacaacactc gtttacgcat
    32521 agctatgcag aaatccggcc gtttaagtga tgactcacgc gaattactgg cgcgctgtgg
    32581 cattaaaatt aatcttcaca cccagcgcct gatcgcgatg gcagaaaaca tgccgattga
    32641 tattctgcgc gtgcgtgacg acgacattcc cggtctggta atggatggcg tggtagacct
    32701 tgggattatc ggcgaaaacg tgctggaaga agagctgctt aaccgccgcg cccagggtga
    32761 agatccgcgc tactttacct tgcgtcgtct ggatttcggc ggctgccgcc tttcgctggc
    32821 aacgccggtt gatgaagcct gggacggccc gctctcctta aacggtaaac gtatcgccac
    32881 ctcttatcct cacctgctca agcgttatct cgaccagaaa ggcatctctt ttaaatcctg
    32941 cttactgaac ggttctgttg aagtcgcccc gcgtgccgga ctggcggatg cgatttgcga
    33001 tctggtttcc accggtgcca cgctggaagc aaacggcctg cgcgaagtcg aagttatcta
    33061 ccgctcgaaa gcctgcctta tccagcgcga tggtgaaatg gaagaatcca aacagcagct
    33121 gatcgacaaa ctgctgaccc gtattcaggg tgtgatccag gcgcgcgaat caaaatacat
    33181 catgatgcac gcaccgaccg aacgtctgga tgaagtcatc gccctgctgc caggtgccga
    33241 acgcccaacc attctgccgc tggcgggtga ccaacaacgc gtagcgatgc acatggtcag
    33301 tagcgaaacc ctgttctggg aaacgatgga aaaactgaaa gcgctgggtg ccagttcaat
    33361 tctggtcctg ccgattgaga agatgatgga gtgatcgcca tgagctttaa cacaatcatt
    33421 gactggaata gctgtactgc ggagcaacaa cgccagctgt taatgcgccc ggcgatctcc
    33481 gcttctgaaa gcattacccg cactgttaac gatattctcg ataacgtgaa agcacgcggt
    33541 gatgacgccc tgcgggaata cagcgcgaag tttgataaaa ccacggttaa cgcgctgaag
    33601 gtgtctgctg aggagattgc cgccgctagc gaacgcctga gcgacgagct aaaacaggcg
    33661 atggcggtgg cagtaaaaaa tattgaaacc ttccacactg cgcaaaaact gccgccggta
    33721 gatgtagaaa cgcagccagg cgtgcgttgc cagcaggtca cgcgcccggt cgcttcagtt
    33781 gggttgtata ttcctggcgg ctccgccccg ctcttctcaa cggtattaat gctggcgact
    33841 ccggcgcgta ttgcgggctg taaaaaagtg gtgctgtgct caccgccgcc gattgccgat
    33901 gagatccttt atgcggcgca gctgtgcggt gtgaaggacg tgtttaacgt cggcggcgca
    33961 caggccattg ccgcgctggc gtttggtacg gaatctgtgc cgaaagtgga caaaatcttc
    34021 gggccgggta acgcctttgt caccgaagcg aaacgtcagg tgagccagcg tctggacggt
    34081 gcggctatcg atatgcccgc aggcccgtcg gaagtgttgg tcattgccga cagcggcgca
    34141 acgccggatt tcgtggcttc tgatttgctt tctcaggctg aacacggccc ggactcacag
    34201 gtgattttac tgacgcctga cgctgatatg gcgcatcaag ttgccgaagc cgtcgaacgc
    34261 cagttagcag aactgccgcg tgccgaaacc gcccgccagg cactgaacgc cagccgcctg
    34321 atcgtgacta aagatttagc gcagtgcgta gagatctcca accagtacgg cccggagcac
    34381 ctgatcatcc agacccgcaa cgcccgcgat ctggtcgatg gcatcaccag cgccggttcg
    34441 gtatttcttg gtgactggtc accggaatcg gcaggtgatt acgcctccgg caccaaccac
    34501 gttctgccga cttacggtta caccgccacc tgttccagcc tcgggctggc agatttccag
    34561 aagcgtatga ccgtgcagga actgtcgaaa gaaggtttct ccgcgctggc ttcgaccatt
    34621 gaaacattgg ccgccgccga gcgcctgacc gcccataaaa atgccgttac tttgcgtgtt
    34681 aacgccctta aggagcaagc atgagcaccg tgactattac cgatttagcg cgtgaaaacg
    34741 tccgcaacct gacgccgtat cagtcggcgc gtcgtctggg cggtaatggc gacgtctggc
    34801 tgaacgccaa cgaatacccc actgccgtgg agtttcagct tactcagcaa acgctcaacc
    34861 gctacccgga atgtcagccg aaagcggtga ttgaaaatta cgcgcaatat gcaggcgtaa
    34921 aaccggaaca ggtgctggtc agtcgtggcg cggacgaagg tattgaactg ctgattcgcg
    34981 ctttttgcga atcgggtaaa gacgccatcc tctactgccc gccaacgtac ggcatgtaca
    35041 gcgtcagcgc cgaaacgatt ggcgtcgagt gccgcacagt gccgacgcag gacaactggc
    35101 aactggactt acagggcatt tccgacaagc tggacggcgt aaaagtggtc tatgtttgca
    35161 gccccaacaa cccgacaggg caactgatca atccgcagga ttttcgcact ctgctggagc
    35221 taacccgcgg aaaagccatt gtggttgccg atgaagccta tatcgagttt tgcccgcagg
    35281 catcgctggc tggctggctg acggaatatc cgcacctggc tattttgcgc acactgtcga
    35341 aagcttttgc tctggcgggc cttcgttgcg gatttacgct ggcaaacgaa gaagtcatca
    35401 acctgctgat gaaagtgatc gccccctacc cgctctcgac gccggttgcc gacattgcgg
    35461 cccaggcgtt aagcccgcag ggaatcgtcg ctatgcgcga acgagtgacg caaattattg
    35521 cagaacgcga atacctgatt gccgcactga aagagatccc ctgcgtagag caggttttcg
    35581 actccgcaac caactacatt ctggcgcgct ttaaagcctc cagcgcagtg tttaaatctt
    35641 tgtgggatca gggcattatc ttacgtgatc agaataaaca accctcttta agcggctgcc
    35701 tgcgaattac cgtcggaacc cgtgaagaaa gccagcgcgt cattgacgcc ttacgtgcgg
    35761 agcaagtttg atgagtcaga agtatctttt tatcgatcgc gatggaaccc tgattagcga
    35821 accgccgagt gattttcagg tggaccgttt tgacaaactc gcctttgaac caggcgtgat
    35881 cccggaactg ctgaagctgc aaaaagcggg ctacaaactg gtaatgatca ccaatcagga
    35941 tggtctggga acacaaagtt tcccgcaggc ggatttcgat ggcccgcaca acctgatgat
    36001 gcagatcttc acctcgcaag gcgtacagtt tgatgaagtg ctgatttgtc cgcacctgcc
    36061 cgccgatgag tgcgactgcc gtaagccgaa agtaaaactg gtggaacgtt atctggctga
    36121 gcaagcgatg gatcgcgcta acagttatgt gattggcgat cgcgcgaccg acattcaact
    36181 ggcggaaaac atgggcatta atggtttacg ctacgaccgc gaaatcctga actggccaat
    36241 gattggcgag cagctcacta aacgagaccg ttacgctcac gtagtgcgca acaccaaaga
    36301 gacacagatt gacgttcagg tgtggctgga tcgcgaaggt ggcagcaaga ttaacaccgg
    36361 tgttggcttc tttgatcaca tgctggatca gatcgccacc cacggtggtt tccgtatgga
    36421 aatcaacgtc aaaggcgacc tctatatcga cgatcaccac accgtcgaag ataccggcct
    36481 ggcgctgggc gaagcgttaa aaatggctct tggcgacaaa cgcggtattt gccgctttgg
    36541 ttttgtgcta ccgatggacg aatgccttgc ccgctgcgcg ctggatatct ctggtcgccc
    36601 gcacctggaa tacaaagccg agtttaccta ccagcgcgtg ggcgatctca gcaccgagat
    36661 gatcgagcac ttcttccgtt cgctctcata caccatgggc gtgacgctac acctgaaaac
    36721 caaaggtaaa aacgatcacc accgtgtaga gagcctgttc aaagcctttg gtcgcaccct
    36781 gcgccaagcc atccgcgtgg aaggcgacac cctgccctcg tcgaaaggag tgctgtaatg
    36841 aacgtggtga tccttgatac cggctgcgcc aatctgaact cggtgaagtc agccattgcg
    36901 cgccacggtt atgaaccaaa agtcagccgt gacccggacg tggtgttgct ggccgataaa
    36961 ctatttttac ccggcgttgg cactgcgcaa gcagcgatgg atcaggtgcg tgagcgcgag
    37021 ctgtttgatc tcatcaaagc ctgtacccaa ccggtactgg gcatctgctt agggatgcaa
    37081 ctgctgggtc ggcgcagcga agagagcaac ggcgtcgact tgctgggcat cattgacgaa
    37141 gacgtgccga aaatgaccga ctttggtctg ccgctgccgc atatgggctg gaatcgcgtt
    37201 tacccgcagg caggcaaccg cctgtttcag gggattgaag acggcgcgta cttttacttt
    37261 gttcacagtt acgcaatgcc ggtcaatccg tggaccattg cccaatgtaa ttacggcgaa
    37321 ccgttcaccg cggcggtaca aaaagataac ttctacggcg tgcagttcca cccggagcgt
    37381 tctggtgccg ctggcgctaa gttgctgaaa aacttcctgg agatgtgatg attattccgg
    37441 cattagattt aatcgacggc accgtggtgc gtctccatca gggcgattac ggcaaacaac
    37501 gcgattacgg taacgacccg ctgccgcgct tacaggatta cgccgcgcag ggtgccgaag
    37561 tgctgcatct ggtggatctg accggggcaa aagatccggc aaaacgtcaa atcccgctga
    37621 ttaaaaccct ggtcgcgggc gttaacgttc cggtgcaggt tggcggcggt gtgcgtagcg
    37681 aagaagacgt ggcggcgtta ctggaagcgg gcgttgcgcg cgtggtggtt ggctccaccg
    37741 cggtgaaatc accagaaagg gtgaaaggct ggtttgaacg tttcggtgcc gatgccttag
    37801 tgctggcgct ggatgtccgt attgacgagc aaggcaacaa gcaggtggcg gtcagcggct
    37861 ggcaagagaa ctcgggcgtt tcactggaac aactggtgga aacctatctg cctgtcggcc
    37921 tgaaacatgt gctgtgtacc gatatctcgc gcgacggcac gctggcaggc tctaacgtct
    37981 ctttatatga agaagtgtgc gccagatacc cgcaggtggc atttcagtcc tccggcggta
    38041 ttggcgacat taacgatgtt gctgccctgc gaggcactgg cgtgcgcggc gtaatagttg
    38101 gtcgtgcatt actggaaggt aaattcaccg tgaaggaggc catcgcatgc tggcaaaacg
    38161 cataatccca tgtctcgacg ttcgtgatgg tcaggtggtg aaaggcgtac agtttcgcaa
    38221 ccatgaaatt attggcgata ttgtaccgct ggcaaaacgc tacgccgaag aaggtgcaga
    38281 cgaactggtg ttctacgata tcaccgcttc cagcgatggc cgtgtggtag ataaaagctg
    38341 ggtatctcgc gtggcggaag tgatcgacat tccgttctgc gtggcgggtg ggattaagtc
    38401 tctggaagat gccgcgaaaa ttctttcctt tggcgcggat aaaatttcca tcaactcccc
    38461 tgcgctggca gacccaacct taattacccg cctggcggat cgctttggcg tgcaatgcat
    38521 tgtggtcggc attgatacct ggtacgacgg cgaaaccggt aaatatcatg tgaatcaata
    38581 taccggcgat gaaagccgca cccgcgtcac tcagtgggaa acactcgact gggtacagga
    38641 agtgcaaaaa cgtggagccg gagaaatcgt cctcaacatg atgaatcagg acggcgtgcg
    38701 taacggttac gatctcgaac aactgaaaaa agtgcgtgaa gtttgccacg tcccgctaat
    38761 tgcctccggt ggcgcgggca ccatggaaca cttcctcgaa gccttccgcg atgccgacgt
    38821 tgacggcgcg ctggctgctt ccgtattcca caaacaaata atcaatattg gtgaattaaa
    38881 agcgtacctg gcaacacagg gcgtggagat caggatatgt taacagaaca acaacgccgc
    38941 gaactggact gggaaaaaac cgacggacta atgccggtaa ttgtgcaaca cgcggtatcc
    39001 ggcgaagtgt taatgctggg ctatatgaac ccggaagcct tagacaaaac catcgaaagc
    39061 ggcaaagtca ccttcttctc gcgcactaaa cagcgactgt ggaccaaagg cgaaacgtcg
    39121 ggcaatttcc tcaacgtagt aaatattgcg ccggactgcg acaacgacac gctactggta
    39181 ctggcgaatc ctatcggacc aacctgccac aaaggcacca gcagctgctt cggcgacacc
    39241 gctcaccaat ggctgttcct gtatcaactg gaacaactgc tcgccgaacg caaatctgcc
    39301 gacccggaaa cctcctatac cgccaaactg tatgccagcg gcaccaaacg cattgcgcag
    39361 aaagtgggcg aagaaggcgt agaaaccgca ctggcagcga cggtacatga ccgctttgag
    39421 ctgaccaacg aggcatcgga cttgatgtat cacctgctgg tgctgttgca ggatcaggat
    39481 ctggatttaa cgacggtaat tgagaacctg cgtaaacggc atcagtgagt tgcagggtaa
    39541 gcggatgcga tgttgttgtc gcatccggca aaaaaaaagg gcaaggtgtc accaccctgc
    39601 ccgttttctt taaagctgca aagattactt cgcgttataa ttacgcagag cgttacgccc
    39661 cagcacaatc cccgcgccaa ccatgccgcc cagcagcacc gccagaatca aggtaattgc
    39721 ctttttcggg ctatcgcgac gaataggtaa cgtcggtttc atcacataac ggtaagcatg
    39781 aatatcaaga tcatcaacct ttaagttttc aatatccaac aggttttgac gtgtctgata
    39841 gtagtttggt gagaacacca acggacgggt cgcctcatgc ttaatcatcg actccagcgc
    39901 ttcgctcccc aaaaggaaca acgtatcttg tgtcacatct tcagtctgct gaacctgtgg
    39961 ctttgccacc tgcgcctgat tcgcatactg caacgcttcc tgaatctgac ggatacgcag
    40021 atctttctgc tcctgcgcga ctacttcctg cgttcttaaa gagtcctgca agtttttccg
    40081 tcccagagca atgttgtctt tgagatcctt ttctagctct tgattcactt tatcatcaac
    40141 ttgctgaatg tattgggcca acttcatttg tgctccctct gcagtttgcc caacataaga
    40201 aacagtcaat ggtaattgct ggttcttaac agaaggttcg atggtaagtt tttctggttc
    40261 ttcctgatta tccagcgttt ctgctaatgc agagaaggca gaactgaagc gaccaattaa
    40321 cgtctcctgt aaatccgata ctttcggtgc agcctgacca tagataacat tcatggcatt
    40381 gttatagcca gcaatttgcc ccacgtcggg ctgagtgata attgctgttg acgtccattt
    40441 ctccttcgct actgccaaat atccaatagc cagggcaata gccacaatga cggaaatgat
    40501 gattgtcatc ttgccacgcc acaactgcgc tagtaaatca atcaaatcaa tctgttccgg
    40561 gtcatggttt tgcccagaaa cattattatt ttctactctc atcattaccc taactgacgg
    40621 aaatttccaa gtccgcatag tttagcgata atcctagtga atgctatagg ataagtgata
    40681 aaaataatga attattgggg aggtaacaaa agacaatcag ggcgtaaata gccctgataa
    40741 caagatgtta gtcgctgcca aagagatcgc gggtgtatac cttatctgcc acatctttaa
    40801 gctcttctgc cattcggtta gagataatga cgtcggcttg ttgtttgaag atggcgagat
    40861 cacgttccag ccgagagttg aagaatgagt cttctttcat cactggctcg tagatgatca
    40921 cttcaacgcc tttcgccttg atacgcttca taatcccctg aatagaagac gcacggaagt
    40981 tatctgaacc gctcttcata atcagacgat aaatacccac cacttgcggt ttgcgtgaca
    41041 aaatggcatc ggcaataaaa tccttacgcg tgcggtttgc atcgacaatt gccgagatca
    41101 ggttattcgg cacagactga tagttcgcca gtaactgctt ggtatctttc ggcaagcaat
    41161 aaccaccata accaaacgac gggttgttgt aatggttgcc aatacgcgga tcgagacaaa
    41221 cgccttcgat aatctggcga gtattcagac ctaaactttc tgcgtagcta tccagttcgt
    41281 taaagtatgc cacgcgcatc gctaggtagg tgtttgcaaa aagtttaatc gcttctgctt
    41341 cggtcgaatc ggtgaacagg gtcgggatat tttgcttaat cgcgccttcc tgtaacaacg
    41401 cagcaaaacg ttctgcgcgt tctgaacgct caccgatgac aatacgtgaa ggatggagat
    41461 tatcgtaaag ggctttacct tcacggagaa attccggaga gaaaataata ttttcagtac
    41521 gatatttctt atgcatcgct tcggtaaaac caacgggaac cgttgatttg atgaccataa
    41581 ccgcataagg atttatctca actacgtctt taattactga ttctacactg gatgtattga
    41641 aataattagt tttaggatca tagtcggttg gagtggcgat gatgacataa tcagcatccc
    41701 ggtaagcttc atttttatct aatgtggcat taaagtgtat tttatctgat tgcaaaaact
    41761 gctgaatttc cttatcaaca ataggagata tccgatcatt cagcatagca acgcgtgacg
    41821 gtaaaatatc taatgccaca acctcatgat tttgtgcgat tagaagcccg tttgacaagc
    41881 ctacatagcc agtaccggaa atggtgattt tcatttcatg ctctcagaat taacttaact
    41941 gtgaatcatg atgtttttag catcctgata agagctaaat gttttaacgc tacgttatta
    42001 gctggtcaac gctacgaaaa acaattcaga ttttctttat aagaatagct gatatttatt
    42061 aagttaatat taagcagtga aatttagtct ataagataaa tggcaaaaaa aaagcccggt
    42121 gcaatatacg ccgggcctca attttactgt tggttaaatc agattaatcc agccattcag
    42181 tatggaacac accttcttta tcaatgcgct tataagtatg tgcaccgaaa tagtcacgct
    42241 gtgcctggat caggttcgca ggcagaacag cggcacggta gctatcgtaa taggcaaccg
    42301 cagcggcgaa ggtcggaacc gggataccgt tctgtactgc ataagcaacg acatcacgca
    42361 gcgcctgctg gtagtcatcg gcaatttgct tgaagtacgg agccagcagc aggttagcga
    42421 tctgcggatt ttcggcatat gcatcggtga ttttctgcag gaactgcgca cggatgatgc
    42481 agccagcacg gaaaatcttc gcgatttcgc cgtagttcag atcccagttg tattcttcag
    42541 acgcagcacg cagctgagag aagccctgag cgtaagaaac gattttgccc agatacagcg
    42601 cacggcgaac tttttcgatg aactcagcct tgtcgcctgc tgactgagct tgcggaccag
    42661 agagaacttt agatgcggca acgcgctgat ctttcagaga agagatataa cgtgcaaaca
    42721 cagactcggt aatcagcgac agcggttctc cgagatccag cgcgctctgg ctggtccatt
    42781 taccggtacc tttgttagcc gcttcatcca ggatcacatc aaccaggtag ttaccgtctt
    42841 catctttttt agtgaagata tctttggtga tgtcgatcag gtagctgctc agttcaccgt
    42901 tattccactc ggtaaaggtc tgtgccagtt cttcgttaga gagattcagg ccgcctttca
    42961 ggagagaata ggcttcagca atcagttgca tatcaccgta ttcaataccg ttgtgaacca
    43021 tcttcacata atggcctgcg ccatcagcac cgatataggt aacgcacggt tcgccatctt
    43081 cagcaacggc ggcgattttg gtcaggatcg gcgcaaccag ttcataggct tctttctggc
    43141 caccaggcat gatggacggc cctttcagcg ccccctcttc tccgccggaa acaccggtac
    43201 cgatgaagtt aaagccttcg gcagaaagct cacggttacg acgaatggtg tcctggaaga
    43261 aggtgttacc accatcgatg atgatgtcgc ctttatcgag atatggtttg agcgaatcaa
    43321 tagcagcatc cgtgcctgca cctgctttca ccattaacag gatgcgacga ggcgtttcca
    43381 gagattcaac aaactctttc accgtatagt aaggaaccag tttcttgcct ggattttcgg
    43441 caatcacttc ttccgtcttt tcacgggaac ggttgaaaat agagacggta taaccacggc
    43501 tttcgatgtt gagcgcaagg ttgcgcccca tcactgccat accgactacg ccgatctgtt
    43561 gctttgacat tgtttactcc tgtcaggggg tggtcaccgc cgagtgtgcg gcttaaatgt
    43621 ggtatagatg ttaactcact catagagatg gtgaatagca ttacctttgc tataccacac
    43681 tgaatatctc accttttccg tcttttaaaa cttatttata tgaaattact gaattacttg
    43741 ttcagtaact caagaataag ttttgtcttt tcttccatca gcggcacatc accgcgagat
    43801 tccacattca accgcaccac cggttcggtg ttggaggtgc gcaggttaaa gcgccagtcg
    43861 gcaaaggtca tgctgatgcc atcggtgcga tccaccgcca gcgcctcgcg gctaaaatgc
    43921 tgttccacgc ggttaatcgc ctcaaagggt tgcgccagtt tgctgttgat ctcaccgctt
    43981 gccggaaacg ctgccatccg gtcgcgcacc agttcgccca gcgtttttcc tttcaggcac
    44041 accagttcgg cgaccagcag ccacgggatc atgccgctgt cgcagtaagc gaaatcacgg
    44101 aagtaatggt gggcgctcat ttcaccaccg tagatggcgt cttccttgcg catccgttct
    44161 ttaataaagg cgtgtccggt tttcgacatc accggcgtgc cgcctgcggc agtcaccaca
    44221 tcaacggtgt tccaggagag acgcggatcg tggatgatct tcgcgccggg atttttttcg
    44281 aggaatgctt ctgccagcag gccgacaatg tagtagccct caataaactg ccctttttcg
    44341 tcaaacagga agcagcggtc aaaatcgcca tcaaaagcaa tgcccatatc cgcaccgtgt
    44401 ttgatgaccg cattgcgggt gtcgtcgcgg cattccggca gtagcgggtt aggaataccg
    44461 ttggggaaat tgccgtccgg cgtgttgtgc actttgatta attccacggg tgcgccaagg
    44521 gctttaaagc gggcttcgat ggcgtccacc accggacccg ctgcgccatt cccggagtta
    44581 atcaccagct tgagcggcgt gaggtttttg acattgatat aaccgaacag gtgatcaacg
    44641 taagcgtcac gcagattgat ttgctgatag cgaccgcgtt tggtttcatc aacgggagga
    44701 aagtcgttgg cttctgccag acgctggaca tcgcgcagtc cggtatcacc gctgatcggg
    44761 cgagcccctt cgcgcaccag tttcatgccg ttgtaatcca tcgggttatg gctggcggta
    44821 acttcaatgc cgccatccac gccgagatgg aacgtggcga aatagatctc ttcggtgccg
    44881 gacatgccaa tatccagcac atcgacgccc gcatcctgta aacctctcgc cagcgccagt
    44941 tttaaggttt cgctggtgag gcggacatca ccgcctaaca caatggtttt cggtttgaga
    45001 aattcgccat aggcgcgacc aatgcgccag gcgatatctt cattcagttc ttcgcctaat
    45061 ttcccgcgaa tatcataggc tttaaagcag gttaattttt tcatgtcgtt accctttttc
    45121 aggcaatagt tggccctgac cgaaacggcc aattttgttt ttgtcgttat ttacatctcg
    45181 gtttcgcatt cattgcctga tgcgacgttt acacccgtcc gtagcgatcc gcgaaacgca
    45241 ccacatcatc ctcttcgaga taagagccgg agcgcacttc aattaaatcg agcggaattt
    45301 tccccgggtt ttccaggcaa tgcatcgccc ccagcggaat ataaatggac tcgttttcac
    45361 caagcagttt gatatcacca tcaatggtga cttttgccgt tcccgcgaca accacccagt
    45421 gttccgcgcg gtgatggtgc atctgtaccg acaagccctc gcccggtttc acggtgatgc
    45481 gtttcacctg gtagcggtcg cccgcgtcga tagagtcata tttgccccac gggcgataga
    45541 cttcgcgatg cacccgatgc tcatggcgac catcggcttt gatctgctcg accacttttt
    45601 tcacatcctg caccgcgtta cggtcggcaa tcagcaccgc atctttggtc tgcaccacta
    45661 ccagatcttt caccccgacg gtggttacga ggccagattc ggcgtacaca tagctgtttt
    45721 cagttttgtg attaatcaca tcgccgtggc aaacgttgcc ctcggcggtg tgggcgctaa
    45781 tttcccataa tgaggaccag gagccgacat cgctccagcc cgcatccatc ggcaccacaa
    45841 cggcatctgc cgtacgttcc atgaccgcgt aatccaccga ctcttccggg caggcgagaa
    45901 acgcttcttc atccacacga ataaaatcga gatccggatc gacggcgctc atcgcttttt
    45961 cacaggcatc gagaatatcc ggacgatatt ttttcagttc ttcgagatag cgtccggcgc
    46021 ggaacaggaa catgccgctg ttccagtaat attcgccgct tgccacatag gcctgggcgg
    46081 tttccagatt cggtttttcg acaaactgcg ccacttcaaa ggccaccgta tcctgctcac
    46141 ccgcagacac ttcaccgcga cgaatatagc cataaccggt ttctggtaga tccggcacaa
    46201 tgccgaaggt caccagcttg cccgcttcgg catatggcat ggcattacgc acggcggcac
    46261 ggaacgcgtc ttcatcggca atcacatgat ccgccgccaa taccagcatt aacgggtcgc
    46321 tctccgggct atgacgtttt gccgccagcg ccgccagcgc aatggcaggt gccgtgtttc
    46381 gccctgccgg ttcgagaata atgttctcgg taagtttgtt cagttgacgc agctgttccg
    46441 cgacaataaa gcggtgctgc tcattgcaaa tcaccaccgg gctttcgcac tccacgccgt
    46501 tcaggcggca gatggtggtt tgcagcatgg tgagatcgcc tttcaggcat aaaaactgct
    46561 tggggtaaag tacgcgggaa agcggccata agcggctacc ggagccacct gccatcacaa
    46621 ctggatagag tttcgactgc gccataatta tccccgaata tcatttataa attgacgtaa
    46681 cacgttctct ttatcgagtg tgcgttcggc atattcacgt gccaccgtgt tgtgtttggg
    46741 cagcaggagc gcctgacgga tccccgccac cagcgcctcg accgattccg gttctacgca
    46801 aaccgcaatg cccggaaagg tttcgcaaag ctgccccagt tctgtgtggg cctctgcggt
    46861 aatcaccgcg ttaccgccta ccgccagaat attggtcagt ttcgacggca atacggcatc
    46921 tgccgcgccg cgtttttgca ccaccagatg gcaatcgccc atcttcagca gtgcgggtaa
    46981 agcgtcatac gattgcagcg ggaaaaattg catgttgcgc agtccacgct gctgcgccat
    47041 tttttccagc cgcgctttgc cgccgccctg cccgacaatg gcaaaaatca gcggttcatc
    47101 gcgcagacga tcggcagctt caataacgtt ttccagcccc tgcttttcac caatattgcc
    47161 ggagtaaaga atgatttttt tgttatccgg caggtcaagc tggttacgaa gggcgtcaac
    47221 atcggcatct gcaacatgct gaaaacgggc aatttccgac cagttgggga agaagatgac
    47281 gttttccgcc gccacgcctt tttcgatggc tttattcatc atcgaacgcg aaatcgtgga
    47341 gacgttatcg acgttatgca gtccgctacg ttcgaacgcc gttgccagct gtgccacttt
    47401 gctgcctttg ccttttccgg caaggcccag ccccagcatg gcgtccactt cgtaatcctg
    47461 aatatgcagc acggtacgcg caccagaaag tttcgccagc aggcgcattc ccggcgtgca
    47521 aaacagcgtt ggcaccacgc cgataatgcg atccggcttc cagcgacgtt gcgccatcag
    47581 aggaaagaaa ctgctgacgg caaaactgcc gagatgtaac aggcgtttca gggtgctcgg
    47641 ctgttttggc acgtacagcg ggcagcgcca caccgtggcg gccccctctt ctcgtttgta
    47701 ccgccaggcg gaatagttct cgcccacctg ccactgcggg tagtaaggcg gtgcggtaat
    47761 aacccgcacc tcatgacctt gtgccgccag ccattccacc atctcgccgg tgtatttgcc
    47821 gataccggtt aactccggcg agtagttaat gccgtagacc agtattttca taatccgggt
    47881 actccggcac gcttctcagc gagaaaatag gcgcggctgt tagcgtgaac atcgttactg
    47941 gcgagcagcg cgtccggcgt cagccagcgg taatcgtcat gctgctcatc cggcagtatc
    48001 agctcttctt cagcgactct gaagcgaaaa ccgagcacca catagtgagt ggtgaaatcc
    48061 gtgccggaga agttatcgtc ataaaagtgc tgccagacac cgtaaaactg gcctgctgtt
    48121 atcggcagac gcagccccag ttccgccatc gtcagccgct cgaatgcggc ttccagcgtt
    48181 tcgtctttct gcacgcgccc tcccggcaca aaccagtaac cctgcgccgg gcggttggtt
    48241 cttttgccaa gcagaaactc accgcgactg ttctcgacaa taaagtcgag agagacaagc
    48301 ggggtggagc gcactaccgt ggcaaagtct tcctgacgta aaaacatcat tacccccgaa
    48361 agcggtcttg attctcaagg aaccactggt aagtgctggc aagccccgct tccagtgaga
    48421 tttcgtgata ccagccaagc tgatgcaggc gcgtcacatc cagcagtttg cgcggcgtac
    48481 catccggttt gctggcatca aaaaccaccc gacctttgta acccaccact ttggcgatgg
    48541 tttgcgccag ctcgcggata gtgcagtcaa cgcccgtgcc gacgttaatg tgcgacagca
    48601 tcggctgggt gttctccagc cagacttcgt gcgccagctc catgacatga atgctcgccg
    48661 ccgccatatc atcgacgtgc aggaattcac gcatcggtgt accgctgccc cataccacca
    48721 cgtccggtgc attctgtgcc gtcgcctcgt ggaagcgacg cagcaatgca gggatcacat
    48781 gcgaattact cgggtggaag ttgtcatgcg ggccatacag gttggtcggc atgactgagc
    48841 ggtaatcgcg tccgtactgg cggttgtatg attcgcacag tttgatcccg gcgattttgg
    48901 caatggcgta aggctcgttg gtcggctcca gcgtgccctg caataactcg ctttctgcca
    48961 tcggctgttt tgccagtttc gggtagatac aggacgatcc aagaaacagc agtttgttca
    49021 cgtcgttctg atgcgcggcg tgaatgatgt tgctctcaat catcatgttc tggtagatga
    49081 aatccgccgg ataggtgttg ttggcaacaa tgccgcccac tttcgccgcc gccagataga
    49141 cctggtcaat gcgttcgctg gcaaagaaat catgcaccgc gcggctgtcc agcaggttca
    49201 gctcgtcgcg ggtgcgtaat accagttcca catcaccgcg ctgttcgagc tgccgcctga
    49261 tggcagaacc gaccatccca cgatggccag cgataaaaat gcgttgttta ctcatgctta
    49321 tgactccagc gcgatcgcca cgtcgtagcc gtgagatttc agcagagagt gttttttcgc
    49381 cgcttcgagg tcattagcca ccatttcaga caccatctct ctgagggtga tttccggttt
    49441 ccagcccagt ttttcgtgcg ctttggtcgg gtcgccgagc agcgtttcaa cttcagccgg
    49501 acggaagtaa cgcggatcaa cggcgataat cacatcaccc ggtttaacgc ccggcgcgtc
    49561 atgcccggtg acggaaacca caatgccctt ctcttcaaca cccgtgcctt caaagcgcag
    49621 tttgatgccc agctgtgccg ctgccatttc cacgaactga cgcacggagt actgaacgcc
    49681 ggtcgcgata acgaaatctt ccggctgttc ctgttgcagc atcatccact gcatttttac
    49741 gtagtctttg gcgtggcccc agtcacgcag ggaatccata ttgccgaggt acaggcacga
    49801 ctccagcccc tgggcgatgt tggcgattgc gcgggtgatt ttgcgggtaa cgaaggtttc
    49861 gccgcggcgc ggggattcat ggttgaagag aattccgtta caagcgtaca tgccgtagga
    49921 ttcgcggtag ttaacggtga tccagtaggc gtacagtttg gcgaccgcat acggagatcg
    49981 cgggtagaac ggcgtggttt ctttctgcgg aatttcctgc accagaccat acagttcaga
    50041 ggtggaagcc tgatagaaac gagttttctt ttccagaccg aggaagcgga tcgcctccag
    50101 caggcgcagc gtacccatcg cgtcgacgtc agcggtatat tctggtgact caaaagagac
    50161 cgcaacgtgg ctcattgcgc ccaggttgta cacttcatcc ggctgtactt cacgcaaaat
    50221 gcgcgtcagg ttagaggtat cactcaggtc gccataatgc agatggaatt tcgggttgca
    50281 ggtgtgcgga tcctgataaa tgtgatccac gcgctcggtg ttgaatgacg atgcgcgacg
    50341 cttaatacca tgcacctcgt aacctttttc cagcagaaac tctgccaggt aagaaccgtc
    50401 ttgtccggtt acaccggtga tgagagcgac ttttgacatg tcttattcct ctgtattttt
    50461 gaatttattc agtttcaacg cgttcgcgta tcaccactgc gggattcccc cggcaaacca
    50521 catttgccgg aagcgattta aaaacactgc ttcgtgcacc cacgacggtg ccgtcgccga
    50581 ttgtgacgcc tggggcaaca aagacatcgg ttgccagcca gcatttctcg ccaatcacaa
    50641 taggcgtggc gttaatggtg aaatgttgac ttgcatggtc gtggctaccg gtgcataaat
    50701 aacttttttg cgatatcacc gaatgtgcgc caatggttat ttcaccgagg gtatataaat
    50761 tgacgtcatc gccgacccac gcgtaatcac ctaaggttaa tttccacgga taggtaattt
    50821 ttactgacgg acgaataact acgttttttc ctatttttgc tccgaataaa cgtaataaaa
    50881 aagcccgcca gcgatacaat acttgtggcg accaggcaaa tattgttgcc tgtactgccc
    50941 accataattg cactttaata gcgttgccgc cccggaaccc tttcggcacc gagaaaccgc
    51001 ttaaatcttg cattggtttt ccttatattc agactttgtt atataaggct ttcgtctttg
    51061 aggtagtacg ttggcgtaaa tgccaggata attcagccca aaagccaggc acatgtaata
    51121 tttgtcgttg gacttttttc gcgtcagcac acaattccat attattggtg gtagataccc
    51181 cacccatgga aaattcagac accaggccat tgagtttttt aaatgcataa ccggctttat
    51241 acattttggc tgccagcgcg tagtcggaag aaactttata ttccaggtca taacgccatt
    51301 ttttcaagcc ggatacaggg aaaaatatcg cctgatgact ggcgggcagg ctgtgataaa
    51361 tataccagcc cggtttggcg ctacgtttaa ttttatgccc gtcgccaaaa tccagcagcg
    51421 catcgccggt gatcatcatg ttatcttttt gcatttttaa cttacggaca aaatttgcgg
    51481 catcctgatg aaaaatatcg cccgaattga gaaacaacgc gaacttgcct tgtgccatcg
    51541 taatgccttt gttcatggcg tcgtagatac cgttatctgg ctcgctgaca aagcgtaggt
    51601 tatagatacc attgagattt tccagatact cacgggtgcc gtcattggaa ccgccatcga
    51661 caacaatcca ttcgaagctg atatcttccg cctgcgccag atgcgccagc gaggcatgtg
    51721 ttttgactat cccttcgagg ttacgaaacg cgacagtgat tatgctaagc aacatgttct
    51781 tattcttacc tcgtaatatt taacgctttt cgcaaaataa acggacagac gattaaaaat
    51841 gcatattccg ggctaaatat cgaaccggta aaaaacagcg ataccggagt aaaaagatat
    51901 aattgcacgc gaaaattgcg gttatcacca aaagcgttga tcatcatttt tatcactttc
    51961 cccatgtacc acagcgataa aaacactgcg aaccaggaaa aataaataat cagcagatac
    52021 aaaccattgt ctatggtttt tccgacatcc gcaccgttaa atattccgaa tgatgcgacg
    52081 tattcataaa gtgagccaaa tctgactaca ccgtcaatat gggtcaaaga ataaccgacc
    52141 atcaccagcg gaccgacaat acgataatac gatgacgatc cttccgtacc taaatcgccc
    52201 agacgggtgg aaatataagg aaacgcgatt accacaccaa cccggaatac agccagagaa
    52261 atcaatgcca acggtaactt tttcttaatc gcctctttat tcagatattg aaatgcccac
    52321 tccagcaaat aaaacaggat aaaagtcata acccctgaaa acgatccgga taatattatc
    52381 cctgcgagaa tcatagcatc ggttttaggc gttttgatac caaactgttt gatgctgagc
    52441 caaattgaga ttaatgccag agcgaaaaat gccggttcga aataaagtgc tgtcgtgcgc
    52501 ttgccgccga atttaatgaa attcagtaca tagctgttgc tgtaaatcag atatttcgaa
    52561 atcgactcca taatactgct gccaccggtg aggataattt gcgccatctc caccgccgcc
    52621 agcgccacca ccagcccgat caccagataa aagaaacgta atatcttgcg atggttgtgt
    52681 ggcgaaatcg ttttaaagcg aatactccac accatgccaa taatgatcac aatatagaca
    52741 aacagcatgg ttgaagtgac gtatttactg gcatccagcg actgaccaaa cagatagtta
    52801 aacgccgtga gtcccgcacc aatccctaag gcaatcatca attttttaac gctgatgcgt
    52861 tctaaaaaca gcagtaacaa gacaggtaaa aaagtgacga tggtgatggg gaaactttcg
    52921 ccaagctggg cgattttgac gttaaccagc aggtagatca gcggcagcag caggtagcta
    52981 cagattctga tagaagttga catactcctc cagcatctgt tgtccactgt aggtggcgcg
    53041 gctgcgttgg ctgaactcag ccagcgtggt accaaatatc gcctgcgcga tttccggttt
    53101 gcttaactgc accagttgca gcacgtcttc ttcgtcgacg gttttaccgc cggatttttg
    53161 caacacttcc cgtgccgcgt cgctatgggt ggcaatcacc ggcacgccaa ttgataacgc
    53221 ctcacacaaa atcagcgggt agttatcgac gcgagaactg aataccagcg catccatctg
    53281 attgagcgcg ctcatcaact tgcgcttgtc agtttcaaag ccgtgattaa ccacgttgcc
    53341 agcggtgaac ggcgagaact taccaaaggt atgcagttcg attttgtcgc ccagcgccat
    53401 catctcacgc accagttgct ggttagtctt gccgtcgtaa cgcaagtcat gcgccaccac
    53461 cgcgattttc ggcttgccct gggtttcgcg caccggaggc aagtccgcca gaatcgcttc
    53521 ggttgccata tcaataccat tattgataat ccggcaacgc cctggaccgt acaggctatt
    53581 gaaagcgtca gccacatgct ggctggggga aataaactga cagcccagcg ccagcatctc
    53641 acggaataac tggcgtttgc ccgccaccag ctggtgtgcg cgatcaatct tcaccggcgg
    53701 atagttattt aaggtcgggc atttctggca gcccgttttc cagccttcgc aaccgtcggt
    53761 aaaggcgcag cgtccggtaa cactccagtg gtcgtgcagc gtccagacca gagtgacgtc
    53821 cggtttatgg tttttcactt tttcgcaaaa gcgcaccacg ctcttaagat ttagccagta
    53881 gctgtgcagc acatgaaaat gcaggaccac cggacccggt gtgcgagtaa tggtgcgata
    53941 taactcattg aaattgccaa acagatcgcg attaaacaga cgaaacaggg caatattcgc
    54001 catcgcggtc atccgcggcg tatgtttgat gacctgcgga tagttctgat ggctgacgct
    54061 ctctttgccg cctttgccat aaccgtagac aaaatgtgac gccagcccct gttgcagcgc
    54121 acgctggtgg agatctaacg ccacacctgc tgccccgcct tccgccagtc gcacattaaa
    54181 ttgcagaata ttcatttaat taccttcact cgcgcttttt ctcccaccac cagcgcgttg
    54241 tccgggacag agtcgagcac cacgctgccc gcacccacgg tgacgttgtt accaagcgtg
    54301 atatcaccaa gaataatgac gttggcaccg agttcgacgc cgttgccaat gtgtggacat
    54361 gccatgttat cggcaccacg attgccgata gtgacgccgt ggcgaatggt gaaatcatcc
    54421 cccgctacca cgtttttatt gatcactacg gcgtaaccgt gatggatagt aaagcggcgg
    54481 ccaatggtcg cggcggcctg gatttcataa ccgaaaaagc attcggtgat aatgcgatac
    54541 agcaccagca gcggggccgc ccacagattg ttgaggacgt tctttttgcg ccacaccgaa
    54601 caaaaatgag caacacgata ggcaagaacc atgcagcacg ggcgtaaact ccagctgttg
    54661 gcacgcagat cttccagcat ctttagcgcc cccgaatacc atcagccaga cgtttgccgt
    54721 tacgcaccga cagcagcgtc agcaaagtgc gccaggtcat acgtttattg cggatctggt
    54781 agagggtaaa cagctgatat tttttgcttg cacggtcaaa tttatctttg tgctttcggt
    54841 aaaagtggaa gtacccggag aattttttcg gtgacgaggt gatctgcatt tcaccgtgat
    54901 tgatatgcag gatctgcgtc gcttcttcca ctttccacgg ttcgccgtac tccaccacca
    54961 tccgcaggaa gatgtcgtaa tcctgtgctg ctttcagttc ggtatcgaac aaacactctt
    55021 tgaaacgcca tgcccaagta aagacctgat tgccaatgat attgcgttta tagaacaggc
    55081 ggcgtgagta cggcgatttg ggatacagtg gtaagcttgc cggttgggaa taaacttctc
    55141 cctggcatac gtagtcgtta gcgtacaaaa aggcgtgagt gaccagttgc tgtttatggg
    55201 cgaggaagac gctcagacgg ttgggtgtcc attcgtcatc gtcatcgatc ccggtaatgt
    55261 attccccttg cgccagcata atcgcctggt tacgtaccgc gcacgccccg ctgttaatgt
    55321 cgttgtgaat gtaggtgata cgcggatcgt tgagggcagt gacgtactgt tgcagttgtt
    55381 cccaggaggt ggagcaatca tccacgatga tcatctccca gttgctgtag tcctggcgca
    55441 ggaccgattt tatcgcccga atcgccagtt gttgacggtt ccaggtcggc atatagattg
    55501 aaatcagcgg attctctttc ataggttgtt ctcccggctt atgagctggt ttgcttgccg
    55561 gatgcggcgt gagcgcctta tccggcctac ggggcggtgc gaatgcaggc gtcgttattt
    55621 cgcatccgac ttatattcgt attcgtaata gccataatcc tgatacgcgc tggcgcggcg
    55681 gaagatggag ttcagaatca cccctttcac cggaataccg ttttgctcaa agcggctcag
    55741 actggtttcc acttctttca atgtgttgac cgcatagcgc gccaccatta acgtggttcc
    55801 agcgtgacgc ccgacaattg ctgcatcggt cactgccaga atcggcggcg tatcaatcaa
    55861 caccaggtcg tagtttttac tcgcccagct caccaattcg gcaaagcgtt cgctcatcaa
    55921 cagttcagaa ggatttggcg gcacctgacc gcgcgggatc aggtcaaatt tggcaataga
    55981 ggtcggttta gctgcggtag taatatcgcc ctggccaatc agaatttccg acaggccatt
    56041 gacgttattg gtacccaaca gctcgtgggt gtagcctttg cgcatatcgc agtcgatcaa
    56101 caacacccgt ttattggtct gactgatcac cgccgccagg ttggcgcaga caaaggtttt
    56161 accgattgac gggctcaccc ctgtcatcat caacacattg ttctgcgcct gcatcatcgc
    56221 gaagtgcaga ctggtacgca gactgcggat ggcttcaatc gccagatcgg ttggattccc
    56281 caccgccagt agctggctct gtttatagcg tttaatccct ttgatggttt tgacgctatc
    56341 gcgcgctttc tgccattccg acagcgggat gctggcatag acgctgatac catgttcttc
    56401 cagcacctgc gggctttcga tgccgcgatt aaacaacgag cgcagcagca cacccacgat
    56461 agagagcatc aggccgagga taattgcgcc gaggataatc agtcctttct tcggtttcag
    56521 cacgccaggc tgggtgattg ccgggtcaac aatgcgcaca tcgccgacgg tgctggcttc
    56581 ggtgattttc agctcctgct ctttattcag cagttgcata tagacctgct gaccagactc
    56641 gacatcgcgg gtcaggcgca caatctcctg ctgggttttc ggcatcgccg ttacgcgacc
    56701 gttaagtttg gctttctctt cttccagcgc ctgacgtttc tccagcagcg tgcggtacgc
    56761 cgggtgaact ttggtgtaca gcttggagat ttccgcctct ttaaaggtca gttcgttcaa
    56821 ctgcgcgtcg atgttcacca tcgaatcgag caccgctttc gcttccagcg gcagatcaac
    56881 agaatcttta tcctgacgga aggcattcag tttgttttcg gcaacatcga ggcggctacg
    56941 tacttccggt aactgttgcg cgaggaaagc gaggcttttc gacgcttccg ccgatttgcg
    57001 ctcaatattt tgttcctgat agttacgggc gatgctgtta agaatgtcgc ggatctgttc
    57061 gcgatcttca ccggtataag tcaggctcag tacgcctgcg tctttgccgt tctccgttac
    57121 cgtcaggctg ttttgcagtt ggttgatcat ccccaacgtg gagtatttgg tgacggtaaa
    57181 ctcactgccc gggcgggcgt gaatggcttc aaccatcagc gtgacgcctt cttttttcag
    57241 catctggccc gcttgcccac gggcgctaaa gccgccatca ctgctcaggg tgtagttttt
    57301 attgtccagc acattaagcg taaacacctg gcccgccatc tctttcgggc ggttaaaggt
    57361 agtcactttc accgtctcgt tctggcgtcc catcagtcgc tcccagcccg ccccgaaaat
    57421 cgggaacgtg tttttgctca ctgcaatatc gaggtcgaga tcgtccaccg ttttaccaag
    57481 caccagacgc gaacgaatca actggatctc ggcgtccgat gcaggcggtt tgttggctaa
    57541 tgccgaacca atgtcctgca ctaacgaatt gccgctgttt tgctcgattt gtaccagtgc
    57601 gtcggcacta taaatcggcg ttgcgaagaa ggtgtaaacc acggcacaga gggcaaatac
    57661 agcggtgatg ccaatcaccc accagcgcgc ttcaatgacg gtgccgacca ggcgaccaat
    57721 atcgatttca tcactgcccg ttaccggagc ggcatgttgt tttacttttt ctgtcattct
    57781 tatacctgct ctgcgttcaa tgcctgcgcc cactggcggg cagaccgttc aagtaatgtg
    57841 tacaccgctg caaacgtttc ccggcttttg cgatacggat cggggatttc acattcgtta
    57901 tcccagtgac caaacagcat cactttgccg cgcatctcag gtgccatctc gcataagcgt
    57961 tcgatatggc gcttttccat ggtcaaaatc aggtcatagt tgcgacacag gcggcggctg
    58021 atttgtcgtg cacagtggcc ttccagagac agttgatgtt ctgcggcgac gctgatagcg
    58081 gtaggatcag cgcccttacc gaccagcgcg ccgagtccag cggactccac tttcagttcc
    58141 gggtgataac gttgcagtaa gcgttccgcc gtcggggaac ggcaaatatt gccgacacag
    58201 acaactaata tgttgttaaa catgacgatt accagttatg aatgtcgctg gctgtatccg
    58261 tcatgtaacg gacaccgcta atagttggca acaactgatt gatcagacgg ttccagcggg
    58321 aaaccggggc ggtggtgaca tacaccacgt cataaggttg caggcggaat tctgtcgcca
    58381 tcaccagcga cgtggcatcg gacatatcca gctggtagat attggcaatc ttgccattac
    58441 gcccgccctc gcctttcagc ggacgaatga caaagatgcc gctggcgttg gaggtggtca
    58501 tgtcgatacc ttcagcatta cccagggctt cagtcagggt catgccgcta aagtccattt
    58561 tcagggtgct ctgtttcttc acttcaccca tcacaaatac tttcagatca tcattacgcg
    58621 gcacgtagag aatatcgccg ggataaagca gtcggttctg attgaggtcg ccgttttgca
    58681 tcagcgcctg caaagaaatg cgttcttcac gaccattgtg tgttagcact acgttgcgcc
    58741 agtcagcggt gtcggtcagg ccacctgcgg cgttgatggc gtcgagaata gtcagtggca
    58801 cgttggtgat cgcctgttga ccggatttat tcacctgacc ggagatatag gccttttgtg
    58861 agcggaaggc ggcgatatta acgtccacct gcgggtcagc gatgtacgtc gctaagcgcc
    58921 cggtaatatc actgcggatt tcagcgagcg ttttcccgac tacgtgaacc ttgccgatat
    58981 acgggtaaaa catagtgccg tcaggctgta cccagttgcc ggtgtcgctg gagctgcggt
    59041 actgaccggc tggcgtggtg agttccgggt gatcccagac ggtgacattc agaacgtcgc
    59101 ccggcccgac gcgatactgg taattcgcga tctcgctttc cagcgtcata ttggggcgcg
    59161 ctacattcgg gcgtgggcgt aattggtcaa tcaggcgcgg ggtcagcgga taaacattca
    59221 ccattttgtc gagatcgaaa tcagcgtcct gctgtttgat gacgtctttg cccatcgtcg
    59281 acatattgct gcccggaagt actgtgcaac cgcttatcaa ggttactgac accaataatg
    59341 gcatcaattt cattttggat ttcatcattg tttatttatc actttggcag agtaattatc
    59401 ctgtgcacta ttaatagcaa tatcgccatg cacatttacc ttgcagttaa ttgaataaaa
    59461 atttaactgg catcagtcct aaaaaaattg atttcatccg caggctattg acagaataat
    59521 tcagactggt ctttcaggca tccagacacg ctaccgcccc tggcttttta gccaccaaca
    59581 cactgattta gcttaatttt tcaaaccctc tctgcataca atcgttgatg agaaagggtt
    59641 attgcggaaa ttaacttccg aatataaggt gacattatgg taattgaata ttggctctcc
    59701 aataatgcag gaggaagtgt tacagctaac ggaatagcag gcaaggtaac aattcggcaa
    59761 ttggctattt ttaagaatta tattaagtgt cattcaatat ggtttttagg agtttcttta
    59821 ggttgacaat atttaatatt gcgcctccac atgcgatatt tcttaaataa tgttttatta
    59881 ttaccacttt taattcaggg ataatgtgag gttattaccc tcaaataata tgagataaat
    59941 agtgctgcaa cattgcattt ttgccccgat ttatccatga tcgaattgtg acatttgtca
    60001 tacaacgaat aggttttgta cttactatgg aatggattgc cgatccgtct atctgggccg
    60061 ggttaatcac gctgattgtg atcgaactgg tcctcggcat tgataacctg gtctttattg
    60121 ccatcctcgc cgaaaaacta ccgccgaggc agcgcgaccg cgcacgggtt accgggctgc
    60181 tgctggcgat gttaatgcgc ctgttactgc tggcgtcaat ctcctggctg gtcaccctga
    60241 ctcaaccgct gttcagcttc cgctcgttta cctttagcgc ccgcgactta atcatgctgt
    60301 ttggtggttt cttcctgttg ttcaaagcca cgatggagct gaacgaacgg ctggaaggga
    60361 aagacagcaa taatcccaca caacgcaaag gtgcgaagtt ctggggcgtg gtgacgcaaa
    60421 ttgtggtact ggacgccatt ttctcactcg actcagtgat taccgccgtc gggatggtcg
    60481 accatttact ggtcatgatg gccgccgtgg ttatcgctat cagcctgatg ttgatggcca
    60541 gcaagccgtt aacgcaattt gttaacagtc acccgacgat cgttattctc tgcttaagct
    60601 tcctgttgat gattggcttt agcctggtgg cagaaggttt cggcttcgtc attccgaaag
    60661 gctacctgta cgctgccatt ggtttctcgg tgatgatcga ggcgctcaat cagttggcta
    60721 tctttaaccg gcgacgtttt ctttccgcta accagacgct gcgccagcgg actaccgaag
    60781 cggtaatgcg cctgcttagc gggcaaaaag aagatgcgga actggacgcc gaaaccgcgt
    60841 ccatgctggt ggatcatggt aaccagcaga tctttaatcc gcaggaacgg cggatgattg
    60901 agcgggtact taatcttaac cagcgtaccg tcagcagcat tatgacgtcg cgccacgata
    60961 ttgagcatat cgatctcaac gcgccggaag aagaaatccg ccagttgctg gagcgaaatc
    61021 agcatacgcg gctggtggtt accgacggtg atgacgcaga agatttgctc ggtgttgttc
    61081 acgttatcga cctgctacaa cagtctctgc gcggcgaacc gctcaacctg cgggtgttga
    61141 ttcgccagcc gctggtgttc ccggaaacct tgccgttgtt acctgccctg gagcagttcc
    61201 gtaatgcccg cacgcacttt gcttttgtgg tggatgagtt tggctcggtg gaagggattg
    61261 tgacattaag tgacgtcact gaaaccattg tcggtaactt accgaacgaa gtggaagaga
    61321 tcgacgcccg ccatgatatt cagaagaatg cggacggttc ctggacggcg aatggtcata
    61381 tgccgctgga agatctggtg caatatgtgc cgctgccgct ggatgaaaaa cgtgaatatc
    61441 acaccattgc cgggctgttg atggaatatt tacagcgtat tccaaaacct ggcgaagaag
    61501 ttcaggtggg ggattatttg cttaaaacgt tgcaggtgga aagccatcgc gtgcagaagg
    61561 tgcagattat tccgctgcat aaggatggcg agttggagta cgaggtgtga tggtatcggt
    61621 cattttaccc aatagccgaa aggaataaga tgagggaacc gcgccagcgg cgatttaccg
    61681 ccgggagccc gggttgccag ggtggtggcg attgagccac cctggcacgt tcaccggctt
    61741 gagcgttcca tagtagcgag gaacatcaag tgaacggaac agccttcgat cttacatatt
    61801 cagaatataa tgatgccgcc acgaacgcct gatgcgacgc tttacgcgtc ttatcaggcc
    61861 tacaggactg gtcacctggt aacctacatc ttctccagca acttcttcac atccttgcca
    61921 ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc ttcgtcctgt
    61981 agatgtttgc gcagtaactg atccacttgc aggctgtaat tgagttgctg ccagttgcca
    62041 taaacccgca gcggtactgg cgtttctttc aggaaatcaa tcagtttact ttccccgttc
    62101 cagccaccca cgacccgaat atcaaactgg gtatcgcagg tttgatctgc cagattcaac
    62161 atgccttccc ctgtcagcgc cagcacaggc gattgacctt gcatgtcgtt taacgtcacg
    62221 acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gccgcgtcac gttatcaaag
    62281 ttttcagcgg ccttcacatc accgccatta cgctctaccg cttgctgaat catctgctgg
    62341 aagttcatcc cttccatgcg cgtgtcggtc atttcgacat gtgcctgtcc ttgccagttg
    62401 tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag tgacattttt
    62461 ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg taccaatctc aacgttttcc
    62521 agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt gcccggcagt
    62581 gaaacctgtc caccgttaag tttgccctgc agttgagtaa tttccagcaa acccgacttg
    62641 ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac gttactggcc
    62701 tgcaacaaaa tatcagccgt aaagccttgc agtccctgat aggccggttc atcaatacgc
    62761 gaagaaatga ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc ggcaccgttt
    62821 tcaccattcg cggtttcatt aagcgggatg aggttgtcaa gattcagttg cgggaattgc
    62881 agtctcagct gccattccgg tttctctgtc agcgtgacct gtgcttgccc gctcagcgta
    62941 ctgtcattgg cggtcaagct gatttggtta aatgaaaggc gtttatgtga ctcctgccac
    63001 tgggcctgga aactcccctg cccctgaata ccttgttttg gtaaatcggc accctgcaac
    63061 tgccagttaa tttgttcaat agccgccgtt aaatcatgcg gataatcaga agcatccacc
    63121 gtaccgttaa gggatattgt gagatcgcgc tgatcgcgat taacccgccc ggagaactca
    63181 aatgagccac gatgttgggg atcttgttcc atttgcaggc ggatattgcg gattgtcacc
    63241 tgctcgtcat cttcatgctg gaacaccagc acgctgtccg ccaccttaag actggatata
    63301 tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg cggtgcaacc
    63361 ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca gttggatcac tgcccctttt
    63421 agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc cacgtccaga
    63481 cgcatgttgt cggcgcgaac cagtggctgg cttgcgccct gggcggtgag agacattcgc
    63541 ccggagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc gagctgcaat
    63601 tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc attcggattc
    63661 accagcaaca ctaacacaga taacccggcc accagcacga ccaggagtat catcagcgtc
    63721 gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc gattcggctg
    63781 gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta aggtcgcgcc
    63841 gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg acgcagcgcc
    63901 agcggcagct taccggagtt gtagaactcc agcacaatgc aaccagacca gcccggatcg
    63961 atacggtgcg cggtgacgtg caccatcagc cccagacgcg ccaaagagga acgcccgtcc
    64021 agccagccca ccagatcggc tggcagcgtc accgactcca gcgtcaccgc cagcgccagc
    64081 tctcctgggt gaagatagaa cgcctcgccc tcgtcgagaa cgatctcatc gctcatcacg
    64141 cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa cgctgccgtg
    64201 tgaccacgga aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc gccgttaata
    64261 cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca ggcttcaata
    64321 tctcggtcac acagacgcat ggcattttct cctttcgcgc atcactccct taacgccaat
    64381 tacgtcaagg gcataccagg ttatcactga acggtacaca atttatcaag cttattcaaa
    64441 gaactgactt attttcgctt tcaatatatc gatcgcgatg cggtttttcc cgccgcgcgg
    64501 cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca tcgggcgcac
    64561 ggttttttga tattgcgcca tcacggaatc cattgaacgc ccacgctcgt taacgtcacg
    64621 cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg agaagttaag
    64681 ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga tgaccttctt
    64741 cggctcaacc gtcaccgttt ctttcatacg cgtatgttca acatagctgt aaaccggcag
    64801 gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca agcagcaggc tgtgatccat
    64861 cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat ggctttgatc
    64921 tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt gctcacgtaa
    64981 ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc cagcgatacc
    65041 gataatgacg cactgatgag actgatcagt catatattta gcgacctgat taacctggat
    65101 gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca tccgtctgat
    65161 accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacaaagaa ttcccggtgg
    65221 caaattacgt tgatcagttt tatgtaaggt aaaaaatgtt atacgcagtt gcgcaaatta
    65281 tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt ttgtactagc
    65341 ataaacacag aaacgaatac tggcgacctg gtcttgcgga taaagcggta atgagcaaac
    65401 aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc agtttaggtt
    65461 tagtctcgtt tatctttacc cttttctcgc ttgagctttc gcagtttggc acccaactcg
    65521 ccccactgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat gccgggcgca
    65581 tgtggccggg aattgcgctg agctgttcgc tgggaaatat cgccgcatcc atcctgcttt
    65641 tttccaccag ctcgctgaac atgacctgga cgaccatcaa tattgttgaa gccgtggtcg
    65701 gggcagtgct gctacgtaaa ttgctgccgt ggtataaccc cttgcaaaat ctggctgact
    65761 ggctgcgtct ggcactcggc agcgccattg ttccgcctct gttggggggt gttctggttg
    65821 tcctgctgac gcccggagac gatcctctca gggcattttt gatatgggta ctgtcagaat
    65881 ccatcggcgc tctggcactg gtgccgctgg gattgttatt taaaccacac tatctgctgc
    65941 gccatcgcaa cccacggttg ctttttgagt cgctgctcac gttagccatc acactgacgt
    66001 taagctggct ttcgatgctg tatctgccgt ggccttttac tttcattatt gtgctgttga
    66061 tgtggagcgc cgtgcgcctg ccacgaatgg aagccttttt gatcttcctt accacggtga
    66121 tgatggtgtc gctgatgatg gccgcggatc cctccctgct tgctacgccg cgtacgtacc
    66181 tgatgagcca tatgccgtgg ctaccgtttt tgctgatcct gctgcccgcc aacatcatga
    66241 ccatggtgat gtatgccttt cgtgcggaac gcaaacacat ttccgaaagc gaaacccgct
    66301 ttcggaacgc gatggaatat tccgctatcg gtatggcgtt agtgggcacc gagggacaat
    66361 ggctgcaaac caacaaagcg ctctgccagt ttctcgggta cagtcaggaa gagctgcgcg
    66421 gactcacctt tcagcaactg acctggccgg aggatctcaa taaagatctc caacaggttg
    66481 aaaagctgat aagcggtgaa ataaacacct attcaatgga aaaacgctac tacaaccgca
    66541 atggcgatgt tgtctgggcg ttgcttgccg tctcactggt gcgccacacg gatggcacgc
    66601 cgctctattt tatcgctcag attgaagaca ttaacgagct aaaacgcacc gaacaggtca
    66661 atcagcaact gatggagcgc atcacgctgg ctaacgaagc gggcgggatt ggcatctggg
    66721 agagggagtt gaagccgaat gtttttagct gggataagcg gatgttcgag ctgtatgaaa
    66781 ttcctccaca tatcaaaccg aactggcagg tgtggtacga gtgcgtgctg ccggaagatc
    66841 gccagcatgc cgaaaaagtg attcgtgatt cgttgcaatc acgctcgccc tttaaactgg
    66901 aatttcgcat taccgtaaaa gacggtattc gccatatccg cgccctcgcc aaccgggtac
    66961 tgaataaaga aggcgaagtc gaacgtctcc tcggcatcaa tatggatatg accgaagtga
    67021 aacagcttaa cgaggcattg tttcaggaaa aagagcgcct gcacattacg cttgattcca
    67081 tcggtgaagc cgtggtctgt attgatatgg cgatgaaaat tacctttatg aatccagtgg
    67141 cggagaagat gagcggctgg acgcaggaag aagcgttagg tgttccgctc ctgacggtgt
    67201 tgcatattac ttttggcgac aacggaccat taatggagaa catttacagt gccgacacct
    67261 cacgttccgc aatcgaacaa gatgtggtgt tgcactgtcg gagcggcggc agttacgacg
    67321 tgcattacag tattacgccg ttaagtactc tggacggcag caatattggt tcggttctgg
    67381 tgattcagga cgtcaccgaa tcacgcaaaa tgctgcgcca gctgagctac agcgcctccc
    67441 atgatgcact gacgcatctc gccaaccgcg ccagttttga gaaacaactg cgtatcctgc
    67501 tgcaaacggt aaacagtacg catcagcgac atgccctggt gtttatcgat cttgatcgct
    67561 ttaaagcggt gaatgacagc gccgggcatg cggcgggcga cgctttgctg cgcgaactgg
    67621 cgtcgttgat gctgagtatg ctgcgctcca gtgacgtgct ggcgcggctc ggcggtgatg
    67681 aatttggtct gctgctgcca gactgcaatg ttgaaagcgc tcgttttatc gctacacgta
    67741 ttatcagtgc tgtgaatgac tatcacttta tatgggaagg ccgggtacat cgggtaggtg
    67801 ccagcgccgg gattaccttg atcgatgaca acaatcatca ggcggcagaa gtgatgtcgc
    67861 aggctgatat cgcctgttat gcctccaaaa atggtggacg gggccgggtg acggtttacg
    67921 aaccacagca agctgccgca catagcgaac gggcggcgat gtcgcttgat gaacagtggc
    67981 ggatgattaa agagaatcag ttgatgatga tcgcccacgg tgtcgcttcg ccgcggatcc
    68041 cggaagcgcg taatttgtgg ctgatttcgc ttaagctctg gagttgcgaa ggcgaaatta
    68101 ttgatgaaca aacatttcgt cgtagcttca gcgatccggc acttagccat gctcttgacc
    68161 gacgggtatt ccacgaattt ttccagcagg ccgccaaagc ggttgccagt aaaggcataa
    68221 gcatcgccct ccccctttcc gttgccggtt tgagtagcgc cacgctggtg aatgatctgc
    68281 ttgagcagct ggaaaatagc cctctaccac cacggttatt acatctgatt attccggctg
    68341 aagcgatttt agatcacgca gaaagcgtgc aaaaactgca gctggcggga tgtcggatag
    68401 tgctcagcca ggtgggccgc gatctgcaaa tcttcaactc gctgaaagcg aatatggcag
    68461 attacctgct acttgatggt gagttatgcg ccaacgtgca gggtaatttg atggatgaga
    68521 tgctgattac gattattcag gggcacgctc agcgactcgg gatgaaaacc atcgccgggc
    68581 cagtcgtttt acccttagtg atggatacgc tttctggcat cggcgtcgat ctgatttatg
    68641 gtgatgtgat tgccgatgcc caaccgctgg atttgctggt gaatagcagt tatttcgcga
    68701 ttaactgaga gagcgtttgc ccattgcctg atgcgacgct aacgcgtctt atcatgccta
    68761 caaatcgctc attccccaga ccggataagg cgctcgcacc gcatccggcg accaacgtca
    68821 tgcttcgtct ggttgccagc cttccgtata ccagatatgc aacagcgcat aagaacgcca
    68881 gggcttccag cgctcggcat aacggcggat ttgtgccggt gtcattcccg gaaatcgccg
    68941 tttaatcaga taatcatccg gcagaaaaac atctttcgcc tgccagccac gcaaagcaaa
    69001 ataattcgcc gtccagcgcc cgatacccgg aaaagtttgc agcgttttca tcgcctgctc
    69061 cacatcgccc ggaattgtca ttggtaagct gccctccagc gccgcatttg ccagatgaat
    69121 cagtgcctct gcccgtttca acggcatgcc taacgctttt aatgcctgcg ggtcggctgc
    69181 tgccagccgc tgaggcgtgg ggaagcagat atactccgga aaatcatcca gccgttcgcc
    69241 ataaagctgt accactttgg cagtcaattt tgccgccatc gccacgctca ccagttggcc
    69301 taaaatcgcc cgcacaccct gctcaaaagc atcaatacag ccgggtaaac gcaatccggg
    69361 ccgccccgcg cctaactttc ccaacgcacc gttaacaatc tgcgggttac attgcagatc
    69421 aagcaggcgg ctcattttcg ccagacactc tgcggcaaca ggttctaaac ctgcacttaa
    69481 atttatgtgc agagtatggc gggctatatc cggaatagca gtcactacgc cgcgatattc
    69541 gcccaccgcc agactacggg cataataatt gtccgcgacc gtttccacac cgctcaccgc
    69601 acgggcggcg agaaatccca acatccacga ccagtcatac ggcggctgcc agttcagggt
    69661 atacatcgca tctccttgtt aatccgcttt cagcataaac gttattcaga cgttacgctt
    69721 tgctttcata ttccggttgt cgcgacagca acatttcgct aaagtcacgc cccttcttca
    69781 ccggcatggg gattaattcg tgtttattgg ttttgattac ggtacagcaa actgttcagt
    69841 ggcggtcatg cgtgacggta aaccgcaatt actgaaaatg gaaaacgaca gcacgctgct
    69901 gccttcaatg ctttgcgcgc caacgcgtga atcggtaagc gaatggctgt accgccatca
    69961 tgatgttccg gcagacgacg atgaaacgca ggcgctgtta cgtcgggcga ttcgttataa
    70021 ccgcgaagaa gatatcgatg ttacggcgaa aagcgtgcag ttcggtcttt cctcactggc
    70081 acagtacatt gatgatccag aagaagtgtg gtttgtgaaa tcaccaaaat cgttcctcgg
    70141 tgccagcggc ttaaaaccgc agcaggtagc gctgtttgag gatctggtct gcgcaatgat
    70201 gttgcacatt cgccagcagg cgcaggcgca gctgccagaa gcgattactc aggcggtgat
    70261 tggtcgcccg atcaacttcc aggggctggg cggcgatgaa gcaaacgccc aggcgcaaga
    70321 gattctggaa cgcgcggcga agcgtgccgg attcagggac gtggtattcc agtacgagcc
    70381 gatcgcggct gggctggatt acgaagccac cttgcaggaa gaaaaacggg tgctggtggt
    70441 ggatatcggc ggtggtacga ctgactgttc attgctgctg atggggccgc agtggcgttc
    70501 gcgtctcgat cgtgaagcca gtctgctggg tcacagtggt tgtcgtattg gcggtaacga
    70561 tctggatatc gccctggcgt ttaaaaacct gatgccactg ctgggtatgg gtggcgaaac
    70621 cgaaaaaggc atcgccctgc cgatcctgcc gtggtggaat gcggttgcca tcaacgacgt
    70681 acctgcgcag agtgatttct acagtagtgc caacggtcgt ctgcttaacg atctggtacg
    70741 cgatgcccgc gaaccggaaa aagtggccct gttacagaaa gtctggcgtc agcgtttaag
    70801 ctatcgcctg gtgcgtagcg cagaagagtg caaaattgct ctttcaagcg tagcggaaac
    70861 ccgcgcctca ctgccgttta tcagcaatga actggctacg ctgattagcc agcgagggct
    70921 ggaaagcgcc ctcagccagc cactggcgcg gattctggaa caggtgcaac tggccctgga
    70981 taacgcccag gaaaaaccgg acgttatcta cctgaccggc ggtagcgccc gttctccgca
    71041 gattaaaaaa gcgctggcag aacagttgcc gggcattccg attgcaggcg gcgatgactt
    71101 tggctccgtc accgccgggc tggcacgctg ggcggaagtg gtgtttcgtt aattgaaaaa
    71161 tggctcagca gcggcttaaa tctgcctgcg cctgagccag tttttctgct gcctgacgta
    71221 acggttccat caacggcatg gtccgtgctg gagcatgaag gcggaatcgc tgcaactctg
    71281 tcgcgccgac tgtcaacgcc gattccagcc gatgtcgttt ggcggtcatt ttcgccatta
    71341 ccgcttgtct gtctgctggc gttatcgctt cattcggcct gaacacaaaa cggcgttcac
    71401 agttcgcttt ccagtcgata accgcctggg tcagatggtc gccaaaccct ttcacttgct
    71461 taacgccacg acgggtaaca tctgcggctg tttcaatacc aaaagaccgt aacgccgctt
    71521 tacgcgcagg gccaacgccg ggaatagagg caacatcaat aaaaaatccc tccagaaact
    71581 tctgcttctg ccgttccctt gcagtgtcat gaagtgctgc cagagcgcgt ttctcttctt
    71641 ccggtaaccc gagaatttcg tccttcattt tttcgagcat cgtccgtttg gcgataaaac
    71701 cttccaggcc gcctacctgc tggatctggc ggactaaatg gtcatagtcc attttcgcac
    71761 gattaaatgc ctcacggcgt tgctgaactt ctgctttgta cgctttgctt gtcaaactgc
    71821 caataatcca gatagccgcc agcacaggaa ccaaaataat ataacgcggt tctgcctgaa
    71881 ggccgcaaag cagcagcgat aatgctgaca gtgcgatctc aagcagaatg atgtattcgc
    71941 gccgtaacag gcctaaagga agcggcctgc cagtcggttg gaaatgatcg ggtaatggca
    72001 gttgcaatgc tggcggtgct actgacgcca tcaccatcgc ccagactttt gccagcacaa
    72061 aatccccgcc ggtggtaatg acctcttcgc cgagatcaat aaaatagata acaccttgat
    72121 tatccagcgc acaccatggg cagtcagcca aatgaccggg ataaacatgc attgccgaaa
    72181 cggtacattt ctttaactgt tggcgtagag aatccagtgc tgatacccac gctttagccg
    72241 tcggacgccc ggttgccacg ccactttccg taaacgcctg ctgaaacatg gcttcaacat
    72301 cactcggtaa catcgatagc ggaatcgatc gtggcggcgg ttttaaacca cgtcgctgat
    72361 tatctgacgc gtaggcataa cggaaatggg taatatccgt ctccagcgca ttacccgcat
    72421 cagagataag cggcacacca gaataaggat gccggccacc aaacaagacg tgaaaaatca
    72481 gcaacgcaag gccaaaatta tcgtgatttt ctgtgcgttc aaagccgaca aatgacgaca
    72541 aggtttgtag ctctggcggc gtaaaatgcg acacgccgac ttcgcataaa tgcagtgtgc
    72601 cattggcgtt aatctgaaag gagtcgctat caatcaacac cactttgctg tcgcgaccta
    72661 ccataaaact gttctggttt acgtcaccca cgacgtgccc gtgctcgtga accgtagcaa
    72721 aagatgaagc aatattgcgc gcaacataga gtagaaaatc ccacgcacaa tgagggtaac
    72781 gttgacgacg atgtgccggg ctatagatca tatgaatcgg ttctttacca gaaacttttg
    72841 gcatcataaa accgataact tttccgcctc gtccaccgtg aagcgttgcc tgcggccagg
    72901 cgacataatt caacaattgc gcgtcagctg tcgcagccat aaaggcaagt ttgtcctgtt
    72961 ttaaggcggg tggcggcgtg tgataaatct tggcgacgct atcgacaaac tcattgatat
    73021 cataaaccgc gccttcaccg cctttgccca gttcacggcc tggcgtaacg cactcaccag
    73081 tagcagtata taaagtgggt ttcatggtaa ccacagcgcc agtgccaacg ttttatcatc
    73141 atccgtacgc tcgtttaccg ccgggctgga caaaaactgc tttaacaatt caggtaataa
    73201 atcgagttgt tcctgcgttg ctgacgccag tccattgaaa aacggggtaa agaaaggcac
    73261 atgaggagaa ttatccagca tatttaacgc cagccgctga ataccatccg taaatgctgc
    73321 aactttatgc gcacgcccag tgctggtgaa agtttccagt ctggaaacgg catcttcgtc
    73381 ggtaatgaag tgcgtcatgt tggcatattc acccaccatc ggcgtgagag gcaattgcag
    73441 accgtgaccc aaatctacca ccacgccgcc atcgccaatc tgcataatca aggtgccgtc
    73501 aggcgatgaa atcaatccca gaaacgtgca ggcaaaatcg cgaactgcta atgctttggc
    73561 ttctgcctcg gcaaaaagtc gttgacgaat agtcagtacc atatttgtgg cgaggacatc
    73621 attaagcccc aattcaccgt cctgtacttt ttgcaccata aacgccatcg cttcattgac
    73681 ggcgagcgtc gcgccttctc ctccctgcga aacgctacct gcgccatcag caaggaacat
    73741 caccagtaat ggttgctggt cgtttaacca gacaacctgc atctggcagg catcctgaca
    73801 aggcaaatca gcactaatgt gcgatgttcc aacagcggat gcataaacca gacgccagct
    73861 cacactgacg tccagccttt tggcgcttcc agcacaactt ccgttcccgg cgtggagcgg
    73921 gaaaccgaac gcagcgaact ggataaccag ctaaacagtt cacggaattg taatccctgc
    73981 agtggcaaag gctgacgaac gctgatttgt gccagagtct tcatatccgc cccctgaaca
    74041 ccgatggaaa agaaggcaaa cttcttatct tcttccccct gaaacacttt gttggcggca
    74101 gcctgccact catcggttgg agcgccatcg gtgatcagga aaatccacgg gcgataatac
    74161 gaaataccat ttgcgcggta ctcacgtttc ctctcttcaa ccatatccag ggctttggta
    74221 atggcggccc ccataggcgt atccccctgg gcaaacagga tgggcgggaa aaagttagcg
    74281 gcgctggtaa agggttgttc cacatgcacc gggccgaacg tcacaatgcc gagttccact
    74341 ctttttaaag ccagcggatc agcaagcagt tcatcacgaa aggtaaccaa tccggcgtta
    74401 agttcattaa taggtctgcc gctcatcgag ccagaaacat ccagcagcaa aatgcagggg
    74461 caacgtggtt ccgggttact ggcaaaatcg ctggtggcaa acgtgatttg ttcgctcata
    74521 agaaatcctt tcttagagaa ttatcgaggt ccggtatcat accgattgca ataatgaaat
    74581 aagagcaaac gaagaaactt tcagaattag gcccgtcggc gcagaaaatt ttgctattcg
    74641 cctcgcaaga acggagagcc gtcgattgac actaagggcg gagtgacata atttcaggag
    74701 tgagggttag ggagaggttt ccccctcccc ctggtgttct tagtaagcct ggaagctaat
    74761 cactaagagt atcaccagta tgatgacgtg cttcatcata accctttcct tattaaaagc
    74821 cctcttctcc gggagagggt ttcccgtttc agcgtcccgc tgaaatcgtc ggcttacctc
    74881 ctttcgccct gaatgcagtc tatcgctaac gcgtgaacaa aatagtttca tctgtgatta
    74941 ttgcgtaacg actcgcaaag aatatttgct ggcaggatcg cagactacaa agcctgcgga
    75001 ttgacaatct tatcgtgaag gcataatttc aggagtgagg gtagagcggg gtttcccccg
    75061 ccctggtagt cttagtaagc ggggaagctt atgactaaga gcaccacgat gatgagtagc
    75121 ttcatcatga ccctttcctt attaaggccc cttcctcggg aggggctttc ccgtttcagc
    75181 gtcccgctga aatcgtcggc ttacctcctt tcgccctgta tgcagtctat cgctgacttg
    75241 cggataaaat agtttccagt gtgattattg cgtaacgtct cgcaaagaat atttgctggc
    75301 aggatcgcag actgcaaagc ctgcagattg acaatcttat cgtgaaggca tactttcagg
    75361 agtgagggta gagcggggtt tcccccgccc tggtagcctt agtaagctgg ccagctaatg
    75421 actaagagca ccacgatgat gagtagcttc atcatgaccc tttccttatt tatggcccct
    75481 tcctcgggag gggctttccc gtttcagcgt cccgctgaaa tcggcggctt acctcctttc
    75541 gccatgcaag cagtctatcg ctaacgcgta gataaaatag tttcctgtgt tattactgga
    75601 tgcgtgctcg caaatgtgcc cgtcattcag acgattcccg acagtgtttc ataattcctc
    75661 catttttctc ccttattggc tggctacact agtatcattc cgcgaaacgt ttcaggaaga
    75721 gaaactctta acgatgaaag gcagttataa atcccgttgg gtaatcgtgg tggttatcgc
    75781 cgccatcgcc gcattctggt tctggcaagg ccgcaatgac tcccagagtg cagccccagg
    75841 ggcgacgaaa caagcgcagc aatcgccagc gggtggtcgc cgtggtatgc gttccggccc
    75901 attagccccg gttcaggcgg cgaccgccgt agaacaggca gttccgcgtt acctcaccgg
    75961 gcttggcacc attatcgccg ctaatactgt tacggtgcgc agccgcgtgg acggtcaact
    76021 gatggcgtta catttccagg aaggccagca ggtcaaagca ggcgatttac tggcagaaat
    76081 tgaccccagc cagttcaaag ttgcattagc acaaacccag ggccaactgg caaaagataa
    76141 agccacgctt gctaacgccc gccgtgatct ggcgcgttat cagcaactgg caaaaaccaa
    76201 tctcgtctcc cgtcaggagc tggatgccca acaggcgctg gtcagtgaaa ccgaaggcac
    76261 cattaaggct gatgaagcaa gcgtcgccag cgcgcagttg caactcgact ggagccgcat
    76321 caccgcacca gtcgatggtc gcgttggtct caagcaggtt gatgttggta accaaatctc
    76381 cagtggtgat accaccggaa ttgtggtgat cacccagacg catcctatcg atttagtctt
    76441 taccctgccg gaaagcgata tcgctactgt agtgcaggcg caaaaagccg gaaaaacgct
    76501 ggtggtagaa gcctgggatc gcaccaattc gaagaaatta agtgaaggca cgctgttaag
    76561 cctcgataac caaatcgatg ccacaaccgg tacgattaaa gtgaaagcac gctttaataa
    76621 tcaggatgat gcgctgtttc ccaatcagtt tgttaacgcg cgcatgttag tcgacaccga
    76681 acaaaacgcc gtagtgatcc caacagccgc cctgcaaatg ggcaatgaag gccattttgt
    76741 ctgggtgctg aatagcgaaa acaaggtcag caaacatctg gtgacgccgg gcattcagga
    76801 cagtcagaaa gtggtgatcc gtgcaggtat ttctgcgggc gatcgcgtgg tgacagacgg
    76861 cattgatcgc ctgaccgaag gggcgaaagt ggaagtggtg gaagcccaga gcgccaccac
    76921 tccggaagag aaagccacca gccgcgaata cgcgaaaaaa ggagcacgct cctgatgcag
    76981 gtgttacccc cgagcagcac aggcggcccg tcgcgcctgt ttattatgcg tcctgtcgcc
    77041 accacgctgc tgatggtggc gatcttactc gccgggatta tcggttatcg cgccctgccc
    77101 gtttcggcgc tgccggaagt ggactatccg accattcagg tggtcacgct ctacccaggt
    77161 gccagcccgg atgtcatgac ctctgccgtt accgcgccgc tcgaacgcca gttcgggcag
    77221 atgtctggcc tgaaacagat gtcgtcgcaa agttccggcg gtgcgtcagt tatcactttg
    77281 cagttccagc taacattacc gctcgatgtc gccgagcagg aagtgcaggc cgcgattaac
    77341 gctgcgacca acttgttgcc gagcgatctg cctaacccgc cggtttacag caaagtgaac
    77401 ccggcagatc cgccgatcat gacgctcgcc gtcacctcaa ccgccatgcc gatgacgcaa
    77461 gtggaagata tggtggaaac ccgcgtcgcg cagaaaatct cgcagatttc cggcgtcggc
    77521 ctggtgacgc tttccggcgg tcagcgtccg gctgttcgcg tcaaacttaa cgctcaggcg
    77581 attgccgccc tcggcctgac cagcgaaacc gtgcgcaccg ccattaccgg cgctaacgtt
    77641 aactcggcaa aaggtagcct cgacggccct tcccgtgcgg tcacgctttc cgcgaacgac
    77701 cagatgcaat ccgccgaaga gtatcgccag ctaatcatcg cctaccagaa cggcgcgcca
    77761 attcgtctgg gcgatgtcgc aactgtagag caaggtgcag aaaacagctg gctcggcgcg
    77821 tgggcgaaca aagaacaggc cattgtgatg aatgttcagc gccagcccgg tgctaacatt
    77881 atctccaccg ccgacagcat tcggcagatg ctgccacagc tcactgagag tctgccgaaa
    77941 tcggtgaagg tgacagtgct ttccgatcgc accaccaata tccgcgcatc cgtcgatgat
    78001 actcagtttg aattgatgat ggctatcgcg ctggtagtca tgattatcta cctgtttttg
    78061 cgcaatattc cggcgaccat cattcccggt gttgctgtac cgctgtcgtt aatcggcact
    78121 ttcgcggtta tggtgtttct cgatttttca atcaataacc tgacactgat ggcgttaact
    78181 atcgccaccg gattcgtggt cgatgacgcc atcgtggtga tcgaaaacat ttcccgctat
    78241 atcgaaaaag gcgaaaaacc gttggcggcg gcgctcaagg gcgcaggtga aatcggcttt
    78301 accattatct cgctgacctt ctcactgatt gcggtgttga tcccactgct gtttatgggc
    78361 gatatcgtcg ggcgactgtt ccgcgaattt gctattaccc tggcggtagc gattttgatc
    78421 tcagcggtgg tgtcgctgac cctgacaccg atgatgtgcg cgcggatgct cagccaggag
    78481 tcgttgcgta aacagaaccg cttctcccgt gcctcggaaa aaatgttcga caggataatc
    78541 gccgcctatg gtcgtggact ggcgaaagtg ctgaatcatc cgtggctgac cttaagcgtg
    78601 gcactcagca cgctgctgct tagcgtgctg ctgtgggtgt tcattccgaa aggtttcttc
    78661 ccggtacagg acaatggcat tattcagggc actttgcagg caccgcaatc cagctccttt
    78721 accaatatgg cccagcgaca acgccaggtc gcggacgtga ttttgcagga tccggcagtg
    78781 caaagcctga cctcatttgt tggcgttgat ggcactaacc cgtcgctgaa tagtgcacgt
    78841 ttacaaatca acctcaaacc gttggatgaa cgtgatgatc gggtgcaaaa agtcatcgcc
    78901 cgtctgcaaa cggcggtaga taaagtgccg ggcgtcgatc tcttcctgca accaacgcag
    78961 gatctgacta ttgatactca ggtcagccgc acccagtacc agtttacctt gcaggccacc
    79021 tcactggatg cgctcagtac ctgggtgcca cagttgatgg aaaaactcca gcaactgcca
    79081 cagctttctg atgtctccag cgactggcag gaccaagggc tggtggcgta tgtcaatgtt
    79141 gatcgcgaca gcgccagccg tctggggatc agcatggcgg atgtcgataa cgccctgtac
    79201 aacgcgtttg gtcagcgact gatttccact atttatactc aggctaacca gtatcgcgta
    79261 gtgctggagc acaacaccga aattaccccg ggcctcgcgg cgctggatac cattcgcctg
    79321 accagtagcg acggcggcgt ggtgccgtta agctcaattg ccaaagttga acagcgtttt
    79381 gcgccgcttt ccatcaacca tctggatcag ttcccggtaa cgaccatctc ctttaacgtg
    79441 ccggataact attcgctggg cgatgcggtg caggagatta tggacaccga aaagacgctg
    79501 aatctgccgg tggatatcac cacgcaattc cagggcagca ccctcgcctt ccagtcggcg
    79561 ctgggcagca ctgtctggct gattgtcgcg gcggtggtgg cgatgtatat cgtgctcggc
    79621 attctgtacg agagctttat tcacccgatc accattctct cgacgctacc caccgcaggg
    79681 gtcggcgcgc tgctggcatt aatgattgcc ggtagcgaac tggatgtgat tgcgattatc
    79741 ggcattattt tgctgatcgg tattgtgaag aagaacgcca tcatgatgat cgacttcgcg
    79801 ctggctgctg agcgcgagca aggcatgtcg ccgcgcgatg caatctacca ggcttgtctg
    79861 ttgcgttttc gtccgatcct gatgaccacc ctggcggctc tgcttggcgc gctgccgctg
    79921 atgttgagta ccggggtcgg cgcggaactg cgtcgtccgt taggtatcgg catggtcggc
    79981 ggtctgattg tcagccaggt gctgacgctg ttcaccacgc cggtgattta tctgctgttc
    80041 gaccgcctgg cattgtggac caaaagtcgc tttgctcgcc atgaagagga ggcgtaagtg
    80101 aagttttttg ccctcttcat ttaccgcccg gtggcgacga ttttactgtc ggtcgccatt
    80161 accctgtgcg gcatactggg cttccgtatg ctgccggtcg ccccgctgcc gcaggtcgat
    80221 tttccggtga ttatggtcag cgcctcgctg cccggtgcgt caccagaaac aatggcgtct
    80281 tccgttgcca cgccgctgga gcgctcgctt gggcgcattg ccggggtcag cgaaatgacc
    80341 tccagcagtt cgctcggcag cacacgtatt attttacagt ttgattttga ccgggacatc
    80401 aacggcgcag cgcgtgatgt gcaggcggcg atcaacgctg cacaaagttt gctgcccagt
    80461 gggatgccca gccgtccgac ctatcgcaaa gcgaacccgt cggatgcgcc aattatgatc
    80521 ctcacgctga catccgatac ttattcgcag ggtgaactgt acgatttcgc ctcgacgcag
    80581 ctggctccga cgatttcgca aatcgacggt gttggtgatg tcgatgtcgg aggcagctca
    80641 ctgcccgccg tacgcgtcgg gctgaatccg caggcgctgt ttaatcaggg cgtgtcgctg
    80701 gacgacgtac gcaccgccat cagcaatgcc aacgtgcgta aaccgcaggg cgcgctggaa
    80761 gatggcactc accgctggca gatccagacc aatgatgagc taaaaaccgc cgctgaatat
    80821 cagccgttga ttattcacta caacaacggc ggcgcggttc gtctgggcga tgtggcgacg
    80881 gtgaccgact cagtgcagga tgtgcgcaac gccgggatga ccaacgccaa accggctatt
    80941 ttactgatga tccgcaaact gccggaagcc aatattatcc agacggttga cagcatccgg
    81001 gcaaaattac cggagttgca ggaaaccatt ccggcggcga ttgatctgca aattgcccag
    81061 gatcgctccc ccaccattcg cgcctcgctg gaagaagtcg agcaaacgct gattatctcg
    81121 gtggcgctgg tgattctggt ggtgttttta ttcctgcgct cgggtcgcgc cactattatt
    81181 cccgccgttt cggtgccggt ttcgctgatt ggtacgtttg cggcgatgta cctgtgcgga
    81241 ttcagtctca ataacctttc gttaatggcg ctcaccatcg ctactggttt cgtggtggat
    81301 gacgccatcg tggtgctgga aaacattgca cgtcatctgg aagcgggaat gaaaccgttg
    81361 caagccgcac tgcaaggtac tcgcgaagtc ggttttacgg tgctgtcgat gagtctgtca
    81421 ctggtggcgg tgttcctgcc gctgctgttg atgggcggat tgccgggccg actgttacgc
    81481 gagtttgccg tgacgctttc tgtcgccatt ggtatttcgt tgctggtttc tctgacatta
    81541 acgccaatga tgtgtggctg gatgctgaaa gccagcaagc cgcgcgagca aaagcgactg
    81601 cgtggttttg gtcgcatgtt ggtagccctg caacaaggct acggcaagtc actgaaatgg
    81661 gtgctcaatc atacccgtct ggtgggcgtg gtgctgcttg gcaccattgc gctgaatatc
    81721 tggctgtata tctcgatccc gaaaaccttc ttcccggagc aggacactgg cgtgttgatg
    81781 ggcgggattc aggcggatca gagtatttcg tttcaggcga tgcgcggtaa gttgcaggat
    81841 ttcatgaaaa ttatccgtga cgatccggca gtggataatg tcaccggctt tacaggcggt
    81901 tcgcgagtga acagcgggat gatgtttatc accctcaagc cacgcgacga acgcagcgaa
    81961 acggcgcagc aaattatcga ccgtctgcgc gtaaaactgg cgaaagaacc gggggcgaat
    82021 ctgttcctga tggcggtaca ggatattcgc gttggtgggc gtcagtcgaa cgccagctac
    82081 cagtacacgt tgttatccga cgacctggcg gcactgcgcg aatgggagcc gaaaatccgc
    82141 aaaaaactgg cgacgttgcc ggaactggcg gacgtgaact ccgatcagca ggataacggc
    82201 gcggagatga atctggttta cgaccgcgac accatggcac ggctgggaat cgacgtgcag
    82261 gccgccaaca gcctgttaaa taacgccttc ggtcagcggc aaatctcgac catttaccag
    82321 ccgatgaacc agtataaagt ggtgatggaa gtggatccgc gctataccca ggacatcagt
    82381 gcgctggaaa aaatgttcgt tatcaataac gaaggcaaag cgatcccgct gtcgtatttc
    82441 gctaaatggc aaccggcgaa cgctccgctg tcggtgaatc atcagggatt atcggcggcc
    82501 tcgaccatct cgttcaacct gccgaccgga aaatcgctct cggacgccag tgcggcgatc
    82561 gatcgcgcaa tgacccagct tggtgtgcct tcgacggtgc gcggcagttt tgccggaaca
    82621 gcgcaggtgt tccaggagac gatgaactca caggtgatcc tgattatcgc cgccatcgcc
    82681 acggtgtata tcgtgctggg tatcctttac gagagttacg tacacccact gacgattctc
    82741 tccaccctgc cctcggcggg cgttggtgcg ctgctggcgc tggagctgtt caatgccccg
    82801 ttcagcctaa tagccctgat agggatcatg ctattaatcg gcatcgtaaa gaaaaacgcc
    82861 attatgatgg tcgattttgc gctcgaagcc caacggcacg gtaacctgac gccgcaggaa
    82921 gctattttcc aggcctgtct gctgcgtttt cgcccgatta tgatgactac cctggcggcg
    82981 ctgtttggcg cgctgccgct ggtgttgtcg ggcggtgacg gctcggagct gcggcacctc
    83041 ctggggatca ccattgtcgg tggactggta atgagccagc ttctgacgct gtataccacg
    83101 ccggtggtgt atctcttttt cgaccgtctg cggctgcgtt tttcgcgtaa acctaaacaa
    83161 acggtaaccg agtaaatgac agaacttccc gacagcaccc gctggcaatt gtggattgtg
    83221 gctttcggct tctttatgca gtcgctggac accaccatcg ttaacaccgc ccttccctca
    83281 atggcgcaaa gcctcgggga aagtccgttg catatgcaca tggtcattgt ctcttatgtg
    83341 ctgaccgtgg cggtgatgct gcccgccagc ggctggctgg cggacaaagt cggcgtgcgc
    83401 aatattttct ttaccgccat cgtgctgttt actctcggtt cactgttttg cgcgctttcc
    83461 ggcacgctga acgaactgtt gctggcacgc gcgttacagg gcgttggcgg tgcgatgatg
    83521 gtgccggtcg gcaggttgac ggtgatgaaa atcgtaccgc gcgagcaata tatggcggcg
    83581 atgacctttg tcacgttacc cggtcaggtc ggtccgctgc tcggtccggc gctcggcggt
    83641 ctgctggtgg agtacgcgtc gtggcactgg atctttttga tcaacattcc ggtggggatt
    83701 atcggtgcga tcgccacatt gctgttaatg ccgaactaca ccatgcagac gcggcgcttt
    83761 gatctttccg gctttttatt gctggcggtt ggcatggctg tattaaccct ggcgctggac
    83821 ggcagtaaag gtacaggttt atcgccgctg gcgattgctg gcctggtcgc agttggcgtg
    83881 gtggcactgg tgctttatct gctgcacgcc agaaataaca accgcgccct gttcagtctg
    83941 aaactgttcc gcactcgtac cttttcgctg ggcctggcgg ggagctttgc cggacgtatt
    84001 ggcagtggca tgttgccctt tatgacaccg gttttcctgc aaattggcct cggtttctcg
    84061 ccgtttcatg ccggactgat gatgatcccg atggtacttg gcagcatggg aatgaagcga
    84121 attgtggttc aggtggtgaa tcgctttggt tatcgtcggg tactggtagc gaccacgctg
    84181 ggtctgtcgc tgatcaccct gttgtttatg actaccgctc tgttgggctg gtactacgtt
    84241 ttgccgttcg tcctgttttt acaagggatg gtcaactcga cccgtttctc ctccatgaac
    84301 accctgacgc tgaaagatct cccggacaat ctggcgagca gcggcaacag tctgctgtcg
    84361 atgattatgc aattgtcgat gagtatcggc gtcaccatcg ccgggctgtt gctgggactt
    84421 tttggttcgc agcatgtcag cgtcgacagc ggcaccacac aaaccgtctt tatgtacacc
    84481 tggcttagca tggcgtttat catcgccctt ccggcgttca tctttgccag agtgccgaac
    84541 gatacgcatc aaaatgtagc tatttcgcgg cgaaaaagga gcgcgcaatg aagttctggc
    84601 gacctggtat taccggcaaa ctgtttctgg cgattttcgc cacctgcatt gttttgctga
    84661 tcagtatgca ctgggcggtg cgtatcagtt ttgagcgtgg ctttattgat tacatcaagc
    84721 atggtaatga acagcgatta caactgttaa gtgatgcgct tggcgagcag tatgcgcagc
    84781 atggcaactg gcgcttcctg cgcaacaatg atcgctttgt ctttcagatc ctgcgttcat
    84841 ttgaacacga taattcggaa gataaacccg gcccgggtat gccaccgcac ggctggcgta
    84901 cccagttctg ggtggttgat caaaacaaca aagtgctggt tggtccgcga gcgccgattc
    84961 cacctgacgg tacacggcga cccattctgg tcaacggtgc ggaagttggc gcggtgatcg
    85021 cctcccccgt tgagcggtta acgcgcaata ctgatatcaa tttcgataaa caacagcggc
    85081 aaaccagctg gttgattgtc gccctggcaa cgttactcgc ggcacttgcc acttttctgc
    85141 tggcgcgcgg tttactggca ccggtaaaac gacttgtcga tggcacgcac aaactggcgg
    85201 cgggcgattt cactacccgc gtaacgccca ccagtgaaga tgaactgggc aaactggcgc
    85261 aagacttcaa ccagcttgcc agcacactgg agaaaaacca gcaaatgcgg cgcgatttta
    85321 tggccgatat ttctcacgaa ctgcgtacgc cattagcggt gctgcgcggt gaactggaag
    85381 ctattcagga tggcgtgcgc aaattcacgc cggagacggt ggcgtcttta caggcggagg
    85441 tcggtacact gaccaaactg gttgacgatc tccatcagtt gtcgatgt
//
LOCUS       DAJVRF010000002        79333 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJVRF010000002 DAJVRF010000000
VERSION     DAJVRF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 79333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79333
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.002"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            29..951
                     /locus_tag="OQN13_000086"
                     /pseudo
     CDS             29..951
                     /locus_tag="OQN13_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            1156..1887
                     /gene="bluR"
                     /locus_tag="OQN13_000087"
     CDS             1156..1887
                     /gene="bluR"
                     /locus_tag="OQN13_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HCS1613566.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPHPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2108..2512
                     /locus_tag="OQN13_000088"
     CDS             2108..2512
                     /locus_tag="OQN13_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCS1613567.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2565..2675
                     /locus_tag="OQN13_000089"
     CDS             2565..2675
                     /locus_tag="OQN13_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1613568.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
     gene            2911..2955
                     /locus_tag="OQN13_000090"
     CDS             2911..2955
                     /locus_tag="OQN13_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1613569.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            3208..3531
                     /gene="iraM"
                     /locus_tag="OQN13_000091"
     CDS             3208..3531
                     /gene="iraM"
                     /locus_tag="OQN13_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCS1613570.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKKIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(3634..4884)
                     /gene="icd"
                     /locus_tag="OQN13_000092"
     CDS             complement(3634..4884)
                     /gene="icd"
                     /locus_tag="OQN13_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCS1613571.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            5056..5709
                     /gene="rluE"
                     /locus_tag="OQN13_000093"
     CDS             5056..5709
                     /gene="rluE"
                     /locus_tag="OQN13_000093"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCS1613572.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDTEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            5719..6180
                     /gene="nudJ"
                     /locus_tag="OQN13_000094"
     CDS             5719..6180
                     /gene="nudJ"
                     /locus_tag="OQN13_000094"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCS1613573.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            6234..7340
                     /gene="mnmA"
                     /locus_tag="OQN13_000095"
     CDS             6234..7340
                     /gene="mnmA"
                     /locus_tag="OQN13_000095"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCS1613574.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            7376..8017
                     /gene="hflD"
                     /locus_tag="OQN13_000096"
     CDS             7376..8017
                     /gene="hflD"
                     /locus_tag="OQN13_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCS1613575.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            8021..9391
                     /gene="purB"
                     /locus_tag="OQN13_000097"
     CDS             8021..9391
                     /gene="purB"
                     /locus_tag="OQN13_000097"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCS1613576.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            9559..10230
                     /gene="phoP"
                     /locus_tag="OQN13_000098"
     CDS             9559..10230
                     /gene="phoP"
                     /locus_tag="OQN13_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCS1613577.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            10230..11690
                     /gene="phoQ"
                     /locus_tag="OQN13_000099"
     CDS             10230..11690
                     /gene="phoQ"
                     /locus_tag="OQN13_000099"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCS1613578.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            11766..12887
                     /gene="roxA"
                     /locus_tag="OQN13_000100"
                     /gene_synonym="ycfD"
     CDS             11766..12887
                     /gene="roxA"
                     /locus_tag="OQN13_000100"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCS1613579.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(12936..14162)
                     /gene="pepT"
                     /locus_tag="OQN13_000101"
     CDS             complement(12936..14162)
                     /gene="pepT"
                     /locus_tag="OQN13_000101"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCS1613580.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            14412..15548
                     /gene="potA"
                     /locus_tag="OQN13_000102"
     CDS             14412..15548
                     /gene="potA"
                     /locus_tag="OQN13_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCS1613581.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            15532..16389
                     /gene="potB"
                     /locus_tag="OQN13_000103"
     CDS             15532..16389
                     /gene="potB"
                     /locus_tag="OQN13_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCS1613582.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            16386..17180
                     /gene="potC"
                     /locus_tag="OQN13_000104"
     CDS             16386..17180
                     /gene="potC"
                     /locus_tag="OQN13_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCS1613583.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            17177..18223
                     /gene="potD"
                     /locus_tag="OQN13_000105"
     CDS             17177..18223
                     /gene="potD"
                     /locus_tag="OQN13_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCS1613584.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(18379..19200)
                     /gene="cobB"
                     /locus_tag="OQN13_000106"
     CDS             complement(18379..19200)
                     /gene="cobB"
                     /locus_tag="OQN13_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCS1613585.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(19216..20127)
                     /gene="nagK"
                     /locus_tag="OQN13_000107"
     CDS             complement(19216..20127)
                     /gene="nagK"
                     /locus_tag="OQN13_000107"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCS1613586.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(20156..21400)
                     /gene="lolE"
                     /locus_tag="OQN13_000108"
     CDS             complement(20156..21400)
                     /gene="lolE"
                     /locus_tag="OQN13_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCS1613587.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(21400..22101)
                     /gene="lolD"
                     /locus_tag="OQN13_000109"
     CDS             complement(21400..22101)
                     /gene="lolD"
                     /locus_tag="OQN13_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCS1613588.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(22094..23293)
                     /gene="lolC"
                     /locus_tag="OQN13_000110"
     CDS             complement(22094..23293)
                     /gene="lolC"
                     /locus_tag="OQN13_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCS1613589.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGNFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            23555..24628
                     /locus_tag="OQN13_000111"
     CDS             23555..24628
                     /locus_tag="OQN13_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCS1613590.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            24756..28202
                     /gene="mfd"
                     /locus_tag="OQN13_000112"
     CDS             24756..28202
                     /gene="mfd"
                     /locus_tag="OQN13_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCS1613591.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            28346..29302
                     /gene="ldtC"
                     /locus_tag="OQN13_000113"
                     /pseudo
     CDS             28346..29302
                     /gene="ldtC"
                     /locus_tag="OQN13_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(29385..29642)
                     /gene="bhsA"
                     /locus_tag="OQN13_000114"
     CDS             complement(29385..29642)
                     /gene="bhsA"
                     /locus_tag="OQN13_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCS1613592.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            29883..30515
                     /gene="comR"
                     /locus_tag="OQN13_000115"
     CDS             29883..30515
                     /gene="comR"
                     /locus_tag="OQN13_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCS1613593.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(30577..31116)
                     /locus_tag="OQN13_000116"
     CDS             complement(30577..31116)
                     /locus_tag="OQN13_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCS1613594.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(31343..32647)
                     /gene="ndh"
                     /locus_tag="OQN13_000117"
     CDS             complement(31343..32647)
                     /gene="ndh"
                     /locus_tag="OQN13_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCS1613595.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELYNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMKNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(33055..33597)
                     /locus_tag="OQN13_000118"
     CDS             complement(33055..33597)
                     /locus_tag="OQN13_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1613596.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(33620..34645)
                     /gene="nagZ"
                     /locus_tag="OQN13_000119"
     CDS             complement(33620..34645)
                     /gene="nagZ"
                     /locus_tag="OQN13_000119"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCS1613597.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(34656..35480)
                     /gene="thiK"
                     /locus_tag="OQN13_000120"
     CDS             complement(34656..35480)
                     /gene="thiK"
                     /locus_tag="OQN13_000120"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCS1613598.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(35461..36102)
                     /gene="lpoB"
                     /locus_tag="OQN13_000121"
     CDS             complement(35461..36102)
                     /gene="lpoB"
                     /locus_tag="OQN13_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCS1613599.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVKAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(36116..36493)
                     /locus_tag="OQN13_000122"
     CDS             complement(36116..36493)
                     /locus_tag="OQN13_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCS1613600.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(36496..36855)
                     /gene="hinT"
                     /locus_tag="OQN13_000123"
     CDS             complement(36496..36855)
                     /gene="hinT"
                     /locus_tag="OQN13_000123"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCS1613601.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            37189..39378
                     /gene="fhuE"
                     /locus_tag="OQN13_000124"
     CDS             37189..39378
                     /gene="fhuE"
                     /locus_tag="OQN13_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HCS1613602.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(39438..40871)
                     /gene="ptsG"
                     /locus_tag="OQN13_000125"
     CDS             complement(39438..40871)
                     /gene="ptsG"
                     /locus_tag="OQN13_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCS1613603.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(41166..41963)
                     /locus_tag="OQN13_000126"
     CDS             complement(41166..41963)
                     /locus_tag="OQN13_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1613604.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(41974..42978)
                     /gene="holB"
                     /locus_tag="OQN13_000127"
     CDS             complement(41974..42978)
                     /gene="holB"
                     /locus_tag="OQN13_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCS1613605.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(42975..43616)
                     /locus_tag="OQN13_000128"
     CDS             complement(42975..43616)
                     /locus_tag="OQN13_000128"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCS1613606.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(43606..44628)
                     /gene="yceG"
                     /locus_tag="OQN13_000129"
     CDS             complement(43606..44628)
                     /gene="yceG"
                     /locus_tag="OQN13_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCS1613607.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(44631..45440)
                     /gene="pabC"
                     /locus_tag="OQN13_000130"
     CDS             complement(44631..45440)
                     /gene="pabC"
                     /locus_tag="OQN13_000130"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCS1613608.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(45560..46801)
                     /gene="fabF"
                     /locus_tag="OQN13_000131"
     CDS             complement(45560..46801)
                     /gene="fabF"
                     /locus_tag="OQN13_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCS1613609.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(46889..47125)
                     /gene="acpP"
                     /locus_tag="OQN13_000132"
     CDS             complement(46889..47125)
                     /gene="acpP"
                     /locus_tag="OQN13_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS1613610.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(47336..48070)
                     /gene="fabG"
                     /locus_tag="OQN13_000133"
     CDS             complement(47336..48070)
                     /gene="fabG"
                     /locus_tag="OQN13_000133"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCS1613611.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(48083..49012)
                     /gene="fabD"
                     /locus_tag="OQN13_000134"
     CDS             complement(48083..49012)
                     /gene="fabD"
                     /locus_tag="OQN13_000134"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCS1613612.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(49028..49981)
                     /gene="fabH"
                     /locus_tag="OQN13_000135"
     CDS             complement(49028..49981)
                     /gene="fabH"
                     /locus_tag="OQN13_000135"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCS1613613.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(50049..51119)
                     /gene="plsX"
                     /locus_tag="OQN13_000136"
     CDS             complement(50049..51119)
                     /gene="plsX"
                     /locus_tag="OQN13_000136"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCS1613614.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(51200..51373)
                     /gene="rpmF"
                     /locus_tag="OQN13_000137"
     CDS             complement(51200..51373)
                     /gene="rpmF"
                     /locus_tag="OQN13_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCS1613615.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(51425..51946)
                     /gene="yceD"
                     /locus_tag="OQN13_000138"
     CDS             complement(51425..51946)
                     /gene="yceD"
                     /locus_tag="OQN13_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCS1613616.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            52145..52729
                     /gene="yceF"
                     /locus_tag="OQN13_000139"
     CDS             52145..52729
                     /gene="yceF"
                     /locus_tag="OQN13_000139"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCS1613617.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(52841..53800)
                     /gene="rluC"
                     /locus_tag="OQN13_000140"
     CDS             complement(52841..53800)
                     /gene="rluC"
                     /locus_tag="OQN13_000140"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCS1613618.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            54373..57558
                     /gene="rne"
                     /locus_tag="OQN13_000141"
     CDS             54373..57558
                     /gene="rne"
                     /locus_tag="OQN13_000141"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCS1613619.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(57753..58706)
                     /gene="flgL"
                     /locus_tag="OQN13_000142"
     CDS             complement(57753..58706)
                     /gene="flgL"
                     /locus_tag="OQN13_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCS1613620.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(58718..60361)
                     /gene="flgK"
                     /locus_tag="OQN13_000143"
     CDS             complement(58718..60361)
                     /gene="flgK"
                     /locus_tag="OQN13_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCS1613621.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(60427..61368)
                     /gene="flgJ"
                     /locus_tag="OQN13_000144"
     CDS             complement(60427..61368)
                     /gene="flgJ"
                     /locus_tag="OQN13_000144"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCS1613622.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(61368..62465)
                     /gene="flgI"
                     /locus_tag="OQN13_000145"
     CDS             complement(61368..62465)
                     /gene="flgI"
                     /locus_tag="OQN13_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCS1613623.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(62477..63175)
                     /gene="flgH"
                     /locus_tag="OQN13_000146"
     CDS             complement(62477..63175)
                     /gene="flgH"
                     /locus_tag="OQN13_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCS1613624.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(63228..64000)
                     /gene="flgG"
                     /locus_tag="OQN13_000147"
                     /pseudo
     CDS             complement(63228..64000)
                     /gene="flgG"
                     /locus_tag="OQN13_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(64172..64927)
                     /gene="flgF"
                     /locus_tag="OQN13_000148"
     CDS             complement(64172..64927)
                     /gene="flgF"
                     /locus_tag="OQN13_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCS1613625.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(64947..66152)
                     /gene="flgE"
                     /locus_tag="OQN13_000149"
     CDS             complement(64947..66152)
                     /gene="flgE"
                     /locus_tag="OQN13_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCS1613626.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(66177..66872)
                     /gene="flgD"
                     /locus_tag="OQN13_000150"
     CDS             complement(66177..66872)
                     /gene="flgD"
                     /locus_tag="OQN13_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCS1613627.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVLRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(66884..67288)
                     /gene="flgC"
                     /locus_tag="OQN13_000151"
     CDS             complement(66884..67288)
                     /gene="flgC"
                     /locus_tag="OQN13_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCS1613628.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(67292..67708)
                     /gene="flgB"
                     /locus_tag="OQN13_000152"
     CDS             complement(67292..67708)
                     /gene="flgB"
                     /locus_tag="OQN13_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCS1613629.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            67863..68522
                     /gene="flgA"
                     /locus_tag="OQN13_000153"
     CDS             67863..68522
                     /gene="flgA"
                     /locus_tag="OQN13_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCS1613630.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            68598..68891
                     /gene="flgM"
                     /locus_tag="OQN13_000154"
     CDS             68598..68891
                     /gene="flgM"
                     /locus_tag="OQN13_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCS1613631.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALLNEAQQDLQSN"
     gene            68896..69312
                     /gene="flgN"
                     /locus_tag="OQN13_000155"
     CDS             68896..69312
                     /gene="flgN"
                     /locus_tag="OQN13_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCS1613632.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(69352..70887)
                     /gene="murJ"
                     /locus_tag="OQN13_000156"
     CDS             complement(69352..70887)
                     /gene="murJ"
                     /locus_tag="OQN13_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCS1613633.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(70997..71920)
                     /locus_tag="OQN13_000157"
     CDS             complement(70997..71920)
                     /locus_tag="OQN13_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1613634.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDDALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(71922..72569)
                     /locus_tag="OQN13_000158"
     CDS             complement(71922..72569)
                     /locus_tag="OQN13_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCS1613635.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(72580..73164)
                     /gene="rimJ"
                     /locus_tag="OQN13_000159"
     CDS             complement(72580..73164)
                     /gene="rimJ"
                     /locus_tag="OQN13_000159"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCS1613636.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            73400..74608
                     /gene="mdtH"
                     /locus_tag="OQN13_000160"
     CDS             73400..74608
                     /gene="mdtH"
                     /locus_tag="OQN13_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCS1613637.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            74672..75319
                     /gene="grxB"
                     /locus_tag="OQN13_000161"
     CDS             74672..75319
                     /gene="grxB"
                     /locus_tag="OQN13_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCS1613638.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            75453..76013
                     /locus_tag="OQN13_000162"
     CDS             75453..76013
                     /locus_tag="OQN13_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1613639.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            76119..77165
                     /gene="pyrC"
                     /locus_tag="OQN13_000163"
     CDS             76119..77165
                     /gene="pyrC"
                     /locus_tag="OQN13_000163"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCS1613640.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            77239..77484
                     /gene="dinI"
                     /locus_tag="OQN13_000164"
     CDS             77239..77484
                     /gene="dinI"
                     /locus_tag="OQN13_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCS1613641.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            77774..78028
                     /gene="bssS"
                     /locus_tag="OQN13_000165"
     CDS             77774..78028
                     /gene="bssS"
                     /locus_tag="OQN13_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCS1613642.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            78143..79261
                     /gene="solA"
                     /locus_tag="OQN13_000166"
     CDS             78143..79261
                     /gene="solA"
                     /locus_tag="OQN13_000166"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCS1613643.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT        19522 a        20864 c        19657 g        19290 t
ORIGIN      
        1 gcctgctcgc cgttttggca aagcgggaat ggaattattt gatttgcgcc tgcacgagcg
       61 agatgacgtt ttacaggccg tattcgacaa aggcacatca aaatttcagc taacttatga
      121 tgacagagcg ctacaatttt ttcgtacttt tgtccttgca accgaacaat caacctattt
      181 cgagatccct gccgaagact cctggctttt tatcgctgac ggatctgata aagaacttga
      241 ttcctgtacc ctttcaccaa ctataaacga ccactttgcc tttcatccct tatcgcggcg
      301 gataatcgct tttgaagcca ttgtgcaaaa aaatgaagcc catcagccat agcggttggg
      361 cagcgtaaag acggggaaat ctacacagcg gatctcaaaa gtaaggcgct tgcattcgcg
      421 atggcacacg cacttgagct cggtgataaa atgatttcaa tcaatctatt acctatgacc
      481 ctggttaacg aacctgacgc agtctctttt ttacttgatg aaataaaggc caatgctctg
      541 gtgcctgaac aaatcatcgt tgaatttact gaaagtgaag ttatatctcg gtttgatgag
      601 tttgcagaag cgattaaatc gcttaaggct gtcggtatca gtgtagcaat tgatcatttt
      661 ggcgcaggtt ttgccggttt gttactcctg tcacgcttcc agcctgacag aattaaaatc
      721 agtcaggaat tgattaccaa tgttcataaa agcgggccac ggcaggcaat tattcaagcg
      781 atcataaaat gctgtacatc acttgaaatt caagtcagtg ctatgggcgt ggcaacacca
      841 gaagagtgga tgtggcttga atccgcagga attgagatgt ttcagggaga tctgtttgct
      901 aaagctaaat tgaatggtat cccttccata gcgtggccag agaaaaaata attttcagca
      961 cattctttca catgatttca gtaaatcatc gtgaaaatga atacgttatg tttaaattta
     1021 atccacatta aatataatgt ttcatccaat ttagctgaaa tcttatttat actttgtaca
     1081 attttagcgt atattgtaca aagtattatt gggtcgtgta caggcggcgg agatttatga
     1141 ccgcaaggag gaattgtggc ttattacagc attggtgatg ttgctgaacg ttgcgggatt
     1201 aatcctgtca ctctccgggc ctggcaacgc cgctacggtt tgttaaaacc acagcgcagt
     1261 gaaggcggac accgactctt tgatgaagaa gacatacaac gcatcgaaga gatcaagcgt
     1321 tggataagta atggcgtccc tgtaggcaaa gttaaagcat tactggaaac caccagccag
     1381 gatacggaag atgactggaa ccgcctgcaa gaagagatga tgtcaattct tcgtatggct
     1441 aatcctgcca aactacgcgc gagaattatt tcactgggtc gagagtaccc agtagatcaa
     1501 ttgattaatc atgtttacct tcctgttcgc cagcgtctcg tgcttgatca caacacctcc
     1561 cgcattatga gcagtatgtt tgacggcgca ttaattgaat atgcagcagc ctcacttttc
     1621 gaaatgcgcc gtaagcccgg taaagaagcc attctgatgg cgtggaatgt tgaagagaga
     1681 gcacgattgt ggctggaagc atggcgttta tcattgtcag gatggcacat ttctgttctt
     1741 gctgatccca ttgaatcgcc gcacccggaa ctgttcccga cgcaaacatt gattgtctgg
     1801 acaggcatgg caccaacgag aaggcaaaac gaacttttac aacattgggg tgagcaaggt
     1861 tataaagtca tctttcatgc cccctaattt cacctttatc ccgcccctta gacagaatgt
     1921 tataatctga acccacaaca gcatttgtac cggagctggt ccgttgcaaa atgctcaccg
     1981 gaagaattca ggtaatccct ctatcacact ctgtacatat cacagccaaa ggacagacca
     2041 gattgaagtg gtaaaaccga tcgtgcacac tacgcagtct cacaactaac aagaggttat
     2101 tatcctgatg gatcaggtcg ttgtttttca aaagatgttt gagcaagtga gaaaagagca
     2161 aaacttctcc tggttttatt cagaattaaa acatcaccgt attgcacatt acatttatta
     2221 tctggctacg gataacatca gaattattac tcacgatgac acggttttgt tattaagagg
     2281 aaccaggaac ctgttaaaag ttagtacgac caagaaccct gctaaaataa aagaggccgc
     2341 attgcttcat atttacggaa aatctacatt tcgggaatac tgttcaacac tggcaggcgc
     2401 gggcgttttc cgttgggtta ctgatgttaa tcataacaaa cgcagttact acgccattga
     2461 taatacgctt ttatatattg aagatgtaga aaataataaa ccattaatct agctaaagtt
     2521 ggatgcttaa gaaatgcttc ataattcagt aaggcattag cataatggga ataaaagtac
     2581 agagactatc cctatggatg ataaatactg tccttttatt gctacccata aataatcacc
     2641 aggccaatac tatcaaattg atatttgaaa tgtgatcact tgactttcta tacgttattt
     2701 tataacggtt aatatattta taaaaacaac gggtgtgcca tacgcccgtt tcaatactta
     2761 acgcacatgt gttttggttt agtcatcatc cagttatatg tattttagcc aggaacaggt
     2821 taaacctttc ctatataact caaaaattga aaccttattc tcatgtcatg tttatattca
     2881 ttattatcgt tatataaaaa ggcaaccata atgtttagca aattggcacc aagtagcata
     2941 aaggctatgt tttaattaca ggatgttcag tcatttgaat gtataacatt atagctaaac
     3001 aaatctaaaa cgaagtgaat aatttattgt tttcactaaa tctcattttg tttaacatcc
     3061 attgagattc cttgctttaa attttgtttt atataagcaa tcgttttaat taatttattt
     3121 tttgaagggg taatatactc atatgcaaaa taaagaaatg aacatccaaa tgaactatat
     3181 taaataccgt gggataagac ataacaaatg aagtgggtag taattgacac ggtaattcaa
     3241 cctacatgcg gcatatcttt ttcagccata tggggtgata tgaaaaagat catctggtat
     3301 caatctacta tatttcttcc tcctggaagt atatttacac cggttaagct tggtattatc
     3361 cttaaggaaa aagaatatcc tattactatt tataacattg caccattcaa caagaattta
     3421 tggagtttac tcaaaagcag tcaagagtgt cctccaggag aaagcaaaat aacaaataaa
     3481 tgtttacata atagttgcat tataaaaata tgcccatatg ggctcaagta atgggcttca
     3541 tataaatatg ctcgatatgc tattaataat atccacataa ataaaacaac gggcgtgtta
     3601 tacgcccgtt tcaatattta actcatggag agattacatg ttttcgatga tcgcttcacc
     3661 aaactctgaa catttcagca gcttagcgcc ttccatcaga cgttcaaagt cataagttac
     3721 ggttttcgcg ttgatagcac cttccatacc tttaacaatt aaatcagccg cttcagtcca
     3781 ccccatatgg cgcagcatca tctcagcgga gaggatgata gagcctggat ttactttgtc
     3841 ctgaccggca tatttcggcg cagtaccgtg ggtggcttca aacagggcgc attcgtcacc
     3901 gatgtttgcg ccaggggcga taccgatacc gccaacctgc gctgccaggg cgtcagaaat
     3961 gtagtcaccg ttcaggttca tacaggcgat aacatcatat tcagccggac gcagcaggat
     4021 ctgttgcagg aatgcatcag caatcacgtc tttaatgacg atctctttgc cggtgttcgg
     4081 gtttttaact ttcagccacg ggccgccgtc gatcagttca ccgccaaact cttcacgcgc
     4141 cagctggtag ccccagtctt taaacgcgcc ttcggtgaac ttcatgatgt tgcctttgtg
     4201 caccagagtc acagagtcac gatcgttagc aattgcgtat tcgatcgctg cacgaaccag
     4261 acgtttggtg ccttcttccg aacacggctt aataccgata ccacaatgtt ccgggaagcg
     4321 aattttcttc acccccatct cttcacgcag gaatttaatc actttctcgg cgtcggcaga
     4381 gtctgctttc cattcgatac ccgcataaat gtcttccgag ttttcacgga agataaccat
     4441 atcggtcagt tcagggtgtt taaccgggct tggagtgccc tgatagtaac gtaccggacg
     4501 caggcagatg tagagatcca gttcctggcg cagggcaacg ttcagagagc gaataccgcc
     4561 accaacagga gtggtcagcg gacctttaat ggcaacgcga tattcacgaa tcagatcaag
     4621 ggtttcagca ggcagccaga catcctgacc ataaacctgt gtggattttt caccggtgta
     4681 aatttccatc caggagattt tacgctcgcc tttataggct ttctcgactg cagcgtcgac
     4741 cactttcagc atggctgggg ttacatctac accgattcca tcaccttcaa tgtaagggat
     4801 aatcggattt tcaggaacgt tgagtttgcc gttttgcagg gtgatcttct tgccttgtgc
     4861 cggaacaact actttacttt ccattcacct ctccttcgag cgctactggt ttgctcgtct
     4921 gccaccacgt tgcatatgcg tttgcgtcct gcgatacgga tgctttagag caattttttg
     4981 ttaatgattt gtaattggct tgtcaatgct accctattgt ttgccgtcat gaaagatgcg
     5041 cgttattagg ctataatgcg gcaattcata atctctgaaa ataccatgca aaaaacttct
     5101 tttagaaatc accaggttaa gcgattcagc tcgcaacgtt ctactaggcg taaacctgaa
     5161 aaccagccca cgcgtgtgat cctgttcaat aaaccctacg atgttcttcc gcagttcacc
     5221 gatgaagccg gacgcaaaac attaaaagaa ttcatcccgg ttcagggcgt ttatgcagca
     5281 ggtcgtcttg accgcgatac cgaagggtta ctggtgctga ccaataacgg cgcgttgcag
     5341 gcgcgtttaa cccagccggg caaacgcacc gggaaaatct attatgtgca ggtggaaggt
     5401 attcccacac aagacgcact tgaagccttg cgcaatggcg taaccttaaa tgatggccct
     5461 accctgcccg ccggcgcgga gctggttgac gaaccagcgt ggttatggcc gcggaatcca
     5521 ccaattcgtg aacgcaaaag tattcccacc agctggctga agatcacctt atatgaagga
     5581 cgtaatcgcc aggtgcgccg catgaccgcc catgttggtt tccccacgct gcgactgatt
     5641 cgttatgcga tgggtgatta cactttggat aaccttgcca atggcgaatg gcgagaagca
     5701 acagaataag gatgcacaat gtttaaaccg cacgttaccg ttgcttgcgt agtgcacgca
     5761 gagggtaaat ttttagtcgt tgaagagacg attaatggta aagcgttatg gaaccaacct
     5821 gccgggcatc tggaagccga tgaaacctta gtggaagctg ccgcccgtga gctatgggaa
     5881 gaaaccggca tcagcgcgca accgcaacac tttattcgta tgcatcagtg gattgcgcca
     5941 gataaaacgc cgtttttgcg tttcctcttt gccattgagc ttgagcaaat atgcccgacc
     6001 cagcctcatg acagcgatat cgactgctgt cgttgggtca gcgccgaaga aattttaaag
     6061 gcgtcaaatc ttcgttcgcc gctggtggcg gaaagtatcc gttgttatca aagtgggcaa
     6121 cgctatccgc tggagatgat tggcgatttt aactggcctt ttacaaaggg tgtcatctaa
     6181 aaggctgcgt gatagaatac gccgccttga agttcaatgt cgtgagtgat ccaatgtctg
     6241 aaaccgcaaa aaaagtaatc gtcggcatgt ccggcggtgt cgattcctcc gtttctgcct
     6301 ggctgttgca acaacaggga tatcaggttg aaggcctgtt tatgaagaac tgggaagaag
     6361 acgacggtga ggaatattgc acagcggcag cggatctggc tgatgcccag gctgtctgcg
     6421 acaagctcgg cattgaactg cacaccgtta actttgctgc cgagtactgg gacaatgtct
     6481 tcgaactgtt ccttgccgaa tataaagccg gtcgcacgcc gaatccggat attctgtgca
     6541 acaaagagat caaatttaaa gctttcctcg aatttgccgc cgaagattta ggtgccgatt
     6601 atatcgctac cggtcattac gtacgtcggg ccgatgtcga tggcaagagc cgcctgctgc
     6661 gtggtctgga cagcaataaa gaccagagct acttccttta tacgctcagc catgagcaga
     6721 ttgcgcaaag cctgttcccg gtcggcgaac tggaaaaacc gcaggtgcgt aagattgctg
     6781 aagatcttgg tctggtcacc gcgaagaaaa aagactctac cggcatttgc ttcattggcg
     6841 aacgtaaatt ccgcgagttc ctgggccgtt atctcccggg gcaaccgggc aaaatcatta
     6901 ccgtcgatgg cgatgaaatt ggtgagcacc aggggctgat gtatcacact ctcggtcagc
     6961 gtaaaggtct gggtatcggt ggcaccaaag aaggcaccga agaaccttgg tatgtggtgg
     7021 acaaagacgt cgaaaacaac attctggttg tcgctcaggg ccatgaacac ccgcggctga
     7081 tgtctgtcgg gttgattgcc cagcagttgc actgggtcga tcgcgaacca ttcaccggca
     7141 ctatgcgttg cacggtaaaa acccgctatc gccagaccga tatcccttgc accgtcaagg
     7201 cgctggacga tgatcgcatt gaagtgattt tcgatgaacc ggttgccgcc gtgacgccgg
     7261 gccagtctgc cgtcttctat aacggtgaag tgtgcctcgg tggcggtatt attgagcagc
     7321 gtctgccgct gccggtctga ttattatctt tacttaaaac aaggaagcag tgaacgtggc
     7381 aaagaattac tatgacatca ccctcgccct ggccggtatt tgtcagtcgg cacgcctggt
     7441 gcaacaactc gctcaccagg ggcattgtga tgccgatgcg ctacacgtct cactcaacag
     7501 tattattgat atgaacccca gctcgacgct ggcggttttt ggcggtagcg aagccaacct
     7561 gcgcgtcggg ctggaaaccc tgctcggcgt gctcaatgcc agcagtcgcc agggcttaaa
     7621 cgccgaatta acccgctaca cactcagctt gatggtgctt gagcgcaaac tctcctcagc
     7681 gaaaggcgcg ctcgacactc tgggcaaccg gatcaacggc ctgcaacgcc agctcgaaca
     7741 cttcgattta cagtccgaaa cgctgatgag cgcgatggct gctatctatg ttgatgtgat
     7801 cagcccgctt ggcccacgca ttcaggtcac cggttcccct gctgtactgc aaagcccaca
     7861 agtgcaggcg aaagttcgcg caaccctgct ggcaggcatt cgcgccgccg tgctctggca
     7921 ccaggtcggc ggcggacgtc tgcaactgat gttttctcgt aatcgcctga ccactcaggc
     7981 aaaacaaatt cttgctcatt taaccccgga gttgtgatct atggaattat cctcactgac
     8041 cgccgtttcc cctgtcgatg gacgctacgg cgataaagtc agcgcgctgc gcgggatttt
     8101 cagcgaatat ggtttgctga aattccgtgt acaagttgaa gtacgttggc tgcaaaaact
     8161 ggccgcgcac gcagcgatca aggaagttcc tgcttttgct gccgacgcaa tcggttacct
     8221 tgatgcaatt gtcgccaatt tcagcgaaga agatgccgca cgcatcaaaa ccatcgagcg
     8281 taccactaac cacgacgtta aagcggttga gtatttcctg aaagaaaaag tggcggagat
     8341 cccggaactg cacgcggttt ctgaattcat ccactttgcc tgtacttcgg aagatatcaa
     8401 taacctctcc cacgcattaa tgctgaaaac cgcgcgtgat gaagtgatcc tgccgtactg
     8461 gcgtcaactg attgatggca ttaaagatct cgccgttcag taccgcgata tcccgctgct
     8521 gtctcgtacc cacggtcagc cagccacgcc gtcaaccatc ggtaaagaga tggcaaacgt
     8581 cgcctaccgt atggagcgcc agtaccgcca gcttaaccag gtggagatcc tcggcaaaat
     8641 caacggcgcg gtcggtaact ataacgccca catcgccgct tacccggaag ttgactggca
     8701 tcagttcagc gaagagttcg tcacctcgct gggtattcaa tggaacccgt acactaccca
     8761 gattgaaccg cacgactaca tcgccgagct gtttgattgc gttgcacgct tcaacaccat
     8821 tctgatcgac tttgatcgtg acgtctgggg ttatatcgcc cttaaccact tcaaacagaa
     8881 aaccattgct ggtgagattg gttcttccac catgccgcat aaagttaacc cgatcgactt
     8941 cgagaactcc gaagggaatc tgggcctttc caacgcggta ttacagcatc tggcaagcaa
     9001 actgccggtt tcccgctggc agcgtgacct gaccgactcc accgtgctgc gtaacctcgg
     9061 cgtgggtatc ggttatgcgc tgattgcgta tcaatccacc ctgaaaggcg tgagcaaact
     9121 ggaagtgaac cgtgaccatc tgctggatga gctggatcac aactgggaag tgctggctga
     9181 gccaatccag acagttatgc gtcgctatgg catcgaaaaa ccgtacgaga agctgaaaga
     9241 gctgactcgt ggtaagcgcg ttgacgccga aggcatgaag cagtttatcg acggtctggc
     9301 gttgccagaa gaagagaaag cccgcctgaa agcgatgacg ccggcaaact acattggtcg
     9361 cgccatcacc atggttgatg agctgaaata aactcgtatc agtgccggat ggcgatgctg
     9421 tccggcctgc ttattaagat tatccgcttt ttattttttc actttacctc ccctccccgc
     9481 tggtttattt aatgtttacc cccataacca cataatcgcg ttacactatt ttaataatta
     9541 agacagggag aaataaaaat gcgcgtactg gttgttgaag acaatgcgtt gttacgtcac
     9601 caccttaaag ttcagattca ggatgctggt catcaggtcg atgatgcaga agatgccaaa
     9661 gaagccgatt attatctcaa tgaacattta ccggatattg cgattgtcga tctcggattg
     9721 ccagacgagg acggtctgtc actgattcgc cgctggcgta gcaatgatgt ttcactgccg
     9781 attctggtat taaccgcccg tgaaagctgg caggacaaag tcgaagtatt aagtgctggt
     9841 gctgatgatt acgtgactaa accatttcat attgaagagg tgatggcgcg aatgcaggca
     9901 ttaatgcggc gtaatagcgg tctggcttca caggtcattt cgctaccacc gtttcaggtt
     9961 gatctctctc gccgtgaatt atctattaat gacgaagtga tcaaactgac cgcgttcgaa
    10021 tacaccatta tggaaacgtt gatacgcaat aatggcaaag tggtgagcaa agattcgtta
    10081 atgctccaac tctatccgga tgcggagctg cgggaaagcc ataccattga tgtactgatg
    10141 ggacgtctgc gcaaaaaaat tcaggcacaa tatccgcaag aagtgattac taccgttcgc
    10201 ggccagggct atttgttcga attgcgctga tgaaaaaatt actgcgtctt tttttcccgc
    10261 tctcgctgcg ggtacgtttt ctgttggcaa cggctgcggt agtactggtg ctttcgcttg
    10321 cctacggaat ggtcgcgctg atcggttata gcgtcagttt cgataaaact acgtttcggc
    10381 tgttacgtgg cgagagcaat ctgttctata cccttgcgaa gtgggaaaac aataagttgc
    10441 atgtcgagtt acccgaaaat atcgacaagc aaagccccac catgacgcta atttatgatg
    10501 agaacgggca gcttttatgg gcgcaacgtg acgtgccctg gctgatgaag atgatccagc
    10561 ctgactggct gaaatcgaat ggttttcatg aaattgaagc ggatgttaac gataccagcc
    10621 tcttgctgag tggagatcat tcgatacagc aacagttgca ggaagtgcgg gaagatgatg
    10681 acgacgcgga gatgacccac tcggtggcag taaacgtcta cccggcaaca tcgcggatgc
    10741 caaaattaac cattgtggtg gtggatacca ttccggtgga gctaaaaagt tcctatatgg
    10801 tctggagctg gtttatctat gtgctctcag ccaatctgct gttagtgatc ccgctgctgt
    10861 gggtcgccgc ctggtggagt ttacgcccca tcgaagccct ggcaaaagaa gtccgcgaac
    10921 tggaagaaca taaccgcgaa ttgctcaatc cagccacaac gcgagaactg accagtctgg
    10981 tacgaaacct gaaccgattg ttaaaaagtg aacgcgaacg ttacgacaaa taccgtacga
    11041 cgctcaccga cctgacccat agtctgaaaa cgccactggc ggtgctgcaa agtacgctgc
    11101 gttctctgcg tagtgaaaag atgagcgtca gtgatgctga gccggtaatg ctggagcaaa
    11161 tcagccgcat ttcacagcaa attggctact acctgcatcg tgccagtatg cgcggcggga
    11221 cattgctcag ccgcgagctg catccggtcg ccccactgct ggacaatctc acctcagcgc
    11281 tgaacaaagt gtatcaacgc aaaggggtca atatctctct cgatatttcg ccagagatca
    11341 gctttgtcgg tgagcagaac gattttgtcg aggtgatggg caacgtgctg gataatgcct
    11401 gtaaatattg cctcgagttt gtcgaaattt ctgcaaggca aaccgacgag catctctata
    11461 ttgtggtcga ggatgatggc cccggtattc cattaagcaa gcgagaggtc attttcgacc
    11521 gtggtcaacg ggttgatact ttacgccctg ggcaaggtgt agggctggcg gtagcccgcg
    11581 aaatcaccga gcaatatgag ggtaaaatcg tcgccggaga gagcatgctg ggcggtgcgc
    11641 ggatggaggt gatttttggt cgccagcatt ctgcgccgaa agatgaataa atatgtccat
    11701 acttcacgca ttacgttaag catccgttat aatcggttgc agataccagc ctgtggatgc
    11761 ttaacatgga ataccaactc actcttaact ggcccgattt tcttgaacgt cactggcaga
    11821 aacgcccggt ggtgttaaaa cgcggcttta ataattttat tgacccgatc tctccagacg
    11881 agttggcggg tctggcgatg gaaagcgaag ttgacagtcg actggtcagt caccaggatg
    11941 gcaaatggca ggtcagccac ggcccgttcg aaagctacga tcatctcggt gaaaccaact
    12001 ggtcattact ggtacaggca gtgaaccact ggcatgagcc gaccgccgcg ctgatgcgac
    12061 cgttccgtga actaccggac tggcgtattg atgatctgat gatttctttt tctgtacctg
    12121 gcggcggcgt cggcccgcat ctcgatcagt acgacgtgtt tatcattcag ggtaccggac
    12181 gtcgtcgctg gcgagtgggc gaaaagctgc aaatgaaaca gcactgccca catccggatc
    12241 tgttacaggt cgatccgttc gaagccatca tcgatgaaga gctggagcct ggtgatattc
    12301 tttatattcc gccaggattc ccgcatgaag gctacgcgct ggaaaatgcg atgaactatt
    12361 ccgtgggctt tcgcgcgcca aatacgcggg aactgattag tggatttgcc gattatgtgc
    12421 tgcaacgtga actgggcggc aactactaca gcgatcctga tgttccacct cgcgctcatc
    12481 ctgcggatgt tctgcctcaa gagatggata aactgcgtga gatgatgctc gaattgatca
    12541 accagccgga acactttaag caatggtttg gcgagtttat atcccagtca cgtcatgaac
    12601 tggatatcgc gccgccagaa ccgccttatc agccagatga aatctacgat gcgctgaaac
    12661 aaggtgatgt gctggtgcgc ctgggtggtc tgcgcgtatt gcgcattggc gacgacgtgt
    12721 atgccaatgg tgagaagatc gattccccgc accgtccggc actggatgca ctcgccagca
    12781 acattgcgct gactgcggag aattttggcg atgcgctgga agatccgtca ttcctcgcga
    12841 tgctcgcggc gctggtcaat agcgggtatt ggttcttcga agggtaagtt tggatttaag
    12901 ccggatgcgg catcacatgc cgcatccctt ttcgcttact tccgttgcgc cgttaactcg
    12961 gcaatacgga cgatcacctg caccgctttt tccatacctt ccagagtcac aaactcatgc
    13021 ttaccatgat agttgtaacc gccagtgaac aagttcgggc acggtaatcc cataaacgac
    13081 aactgcgcgc cgtcggtacc accgcggatc ggtttcagtt ccggttcaat atcgcagtca
    13141 cgcatcgcct gctgggcgat atcgagaata tgcggatgct caaccacttt ctctcgcata
    13201 ttgtagtaac tgtcttcaat caccagttca atgtagcaat caggatgtaa ccctttgccc
    13261 acttttttgg cgatctccat cattttacgt ttacgcgctt caaactgttt acggtcgaaa
    13321 tcacggatga tgtagtgcat atcggcccgt tcaacggtgc ctttcatgct cgccaggtga
    13381 tagaaacctt catagccttc tgtcatttcc gggctttcat ccgccggaac ttccgcatga
    13441 atacgtgccg ccagcgacag cgcatttacc atcactcctt tcgccgtgcc cggatgaaca
    13501 ttgttaccga caattttgat attgaccgat gcggcgttga agttttcaaa ctccagttcg
    13561 cctacgccac caccgtcaac agtgtaagcc cagcgggcat cgaaggcatc aacatcaaaa
    13621 tgtttcgccc ctttgcccac ttcttcatcc ggggtaaagg cgacgcgaat atcaccatgc
    13681 ggaatgtttt tctgttgcaa taccgccagc gcggtcatga tttctgcaat acctgctttg
    13741 tcatcggcac ctaacaaggt tttaccatcg gtggtaatca acgtctgacc cagtagctga
    13801 tgcagcaccg ggaacataac cggtgataaa acttcatcgc cgatacccag cgcaatatcg
    13861 ccaccgcgat agttttcaac aatttgcgga ttaacatttt tgccgctgca atccggtgag
    13921 gtatccacat gagaaataaa gccaatcgcc gggatatcgc cagggacgtt agccggtaac
    13981 gtcgccatca aagtgccctt ctcacttaag gtcacattga taagccccat ttcttcgagc
    14041 tgctctttca gcagatgcaa taacttccat tggccttccg tgctgggaac ctgtctcacc
    14101 cctgcttttg attgggtatc cagagatacg tagttcaaaa atcgctcaag tagtttatcc
    14161 atgtagtcac cctcactttt tgtgacaaca ttattaagaa gcaagaaaag acaaatattg
    14221 cgtcaggtca cttttacccc tgcaagcggg aatatttatc agcattacct taatgaatat
    14281 aaagctaagc cagtaattca caacaaagat tggcgattca agcggtttgc cgtagaatta
    14341 ccgccctcat taagagtcac caaggtggtt aaccacaaac cccgcatcgg taagccatcc
    14401 gttgcgttta catgggacag agtaaaaaat tgaataaaca accgagttcg ctttcaccgc
    14461 tggtgcaatt ggcgggaatt cgcaaatgct ttgatggtaa agaggtcatt ccccagctgg
    14521 atctgactat caacaatggc gagttcctca cgctgcttgg cccttctggc tgcggtaaaa
    14581 caaccgttct tcgcctgatt gcaggtctgg aaactgttga ttccggacgc atcatgctgg
    14641 ataacgagga catcacccac gttccggcgg aaaaccgcta tgtgaacact gttttccaaa
    14701 gctacgcact tttcccccac atgaccgtgt tcgaaaatgt ggcctttggg ttgcgcatgc
    14761 aaaaaacccc cgctgctgaa attacgcccc gcgtgatgga agccctgcgg atggtgcaac
    14821 tggaaacctt cgctcaacgt aaaccgcatc agctctctgg tggtcaacag caacgcgtcg
    14881 ccattgctcg cgcggtggtt aacaagcctc gtctgttgtt gctggatgag tcgctctcag
    14941 cgctggatta caaactgcgt aagcaaatgc agaacgagct gaaagcgtta cagcgtaagc
    15001 ttggtattac cttcgtcttt gtcactcacg atcaggaaga agcgctcacc atgtcagaca
    15061 ggattgtggt gatgcgcgag ggtcgtattg agcaagatgg tacaccgcgc gaaatttacg
    15121 aagagccgaa aaacctgttt gttgccggct tcattggcga aatcaatatg tttaacgcca
    15181 ctgtcatcga acgtctcgac gagcagcgcg tacgcgccaa cgttgaaggc cgcgaatgta
    15241 atatctacgt taacttcgcc gttgaaccgg ggcaaaaact gcatgttctg ctgcgcccgg
    15301 aagacttacg tgttgaagag attaacgacg acaaccacgc tgaagggctg attggttacg
    15361 ttcgtgagcg taactacaaa ggcatgacgc tggagtcggt tgttgaactg gaaaatggca
    15421 agatggtgat ggtcagcgaa ttcttcaatg aagacgaccc tgactttgac cactctctcg
    15481 accaaaaaat ggccattaat tgggtagaaa gctgggaggt cgtactggct gatgaagaac
    15541 acaagtaagt tccagaatgt agtgattgtc actattgtcg gttggcttgt gttgtttgtc
    15601 tttctgccca acctgatgat cattggcacc agctttttga cccgcgacga cgccagtttc
    15661 gtcaaaatgg tctttacgct ggataactac acgcgtctgc tcgatccgct ctattttgaa
    15721 gtgctattgc actcgctgaa tatggcgctg atcgccaccc tcgcctgcct ggtgctgggc
    15781 tacccgtttg cctggtttct ggcgaagctg ccacacaagg tgcgtccgct gctgctgttt
    15841 ctgctgattg ttcccttctg gaccaactca ctgattcgca tctacgggct gaaaattttc
    15901 ctcagcacca aaggctatct caacgagttt ttgctctggc tgggcgttat cgacacacca
    15961 attcgtatca tgttcacgcc cagtgcggtg attatcggtc tggtttacat tctgctaccg
    16021 tttatggtga tgccgctgta ctccagtatc gaaaaactgg ataagccgtt gcttgaggcg
    16081 gcgcgcgatc tcggtgccag caagttacag acttttatcc gtatcattat tccactgacg
    16141 atgccgggaa ttattgccgg atgtctgctg gtgatgctgc cagcgatggg cctgttctat
    16201 gtatccgacc tgatgggcgg tgcgaaaaac ctgctgatcg gtaacgtcat caaggtccag
    16261 ttcctcaata ttcgtgactg gccgtttggt gcagccacca gcattacgct gactatcgta
    16321 atgggcctga tgttgctggt ttactggcgc gcttctcgtc tgctgaataa aaaggtggaa
    16381 ctcgaatgat cggtcgactg cttcgcggcg gttttatgac cgctatctac gcgtacctgt
    16441 atatcccaat cattattttg attgtgaact cctttaacag ctcgcgcttt ggcatcaact
    16501 ggcagggttt taccaccaaa tggtatagcc tgctgatgaa caacgacagc ctgttacagg
    16561 cagcgcagca ttcactaaca atggcggtgt tttcggcgac atttgctacg cttatcggtt
    16621 cactgacggc agttgcgctg taccgttatc gctttcgtgg taagccgttc gttagcggaa
    16681 tgctgtttgt ggtgatgatg tcaccagata tcgtgatggc gatttctctg ctggtgctgt
    16741 ttatgctgct gggtattcag cttggcttct ggtcgctgct gttctcgcat atcaccttct
    16801 gcctgccgtt tgtggtggtg acggtgtatt cgcgcctgaa aggttttgac gtgcggatgc
    16861 tggaagcggc gaaagatctc ggtgccagtg aatttaccat tctgcgaaaa atcattctgc
    16921 cactggcaat gccagcggtg gcggcgggct gggtgttaag ctttaccctg tcgatggacg
    16981 atgtggtagt ttcttcgttc gtcaccggac caagttatga aattctgccg ttaaaaattt
    17041 attcgatggt caaagtcggc gtatcgccgg aagttaacgc gctggcaacc atattactgg
    17101 tgctgtcgct ggtgatggtt attgccagcc agcttattgc tcgtgataaa acgaaaggta
    17161 acacagggga cgttaaatga aaaaatggtc acgccacctg ctcgcggcgg gtgctctggc
    17221 actgggcatg agcgccgctc acgccgatga caacaacacg ctgtatttct acaactggac
    17281 cgagtacgtg ccgccaggac tgcttgaaca gttcaccaaa gaaaccggta ttaaggctat
    17341 ctattcgact tacgagtcga acgaaaccat gtacgccaag ctgaaaacgt acaaagacgg
    17401 tgcctatgac ctggtggttc cttcaaccta ttacgtcgat aaaatgcgta aagaagggat
    17461 gatccagaag atcgacaagt cgaagttaac gaacttcagc aatctcgatc cagacatgct
    17521 caacaagccg tttgacccga ataacgacta ctccattccg tatatctggg gtgcgacggc
    17581 gattggcgtt aacggtgatg cggtggatcc taaatctgtc accagttggg cagatctgtg
    17641 gaagccagag tacaaaggta gcctgctgct gaccgacgat gcccgtgaag tgttccagat
    17701 ggcgctgcgc aaactgggct actccggtaa caccaccgat ccgaaagaga ttgaagctgc
    17761 atataacgag ctgaaaaaac tgatgccaaa cgtcgcagcg tttaactccg ataacccggc
    17821 taacccgtac atggaaggcg aagttaacct cggcatgatc tggaacggct ctgctttcgt
    17881 tgcacgccag gcgggtacgc caattgacgt ggtgtggccg aaagaaggcg gcattttctg
    17941 gatggacagc ctggcgatcc cggcaaatgc caaaaacaaa gaaggcgcgc tgaaattgat
    18001 caacttcctg ctgcgcccgg atgtggcaaa acaggttgcc gaaactatcg gttatccaac
    18061 gccaaacctt gcggcgcgta agctgttaag cccggaagtg gcgaacgata aaacgctcta
    18121 cccggatgct gaaaccatta aaaatggtga atggcagaat gacgttggcg cagccagcag
    18181 catttatgaa gagtattatc agaagctgaa agcaggacgt taattcagca aacgggcggt
    18241 ggtattaccg cccgtttttt agttagcaag gatgtggcta atgagtcagg acagtaaagt
    18301 tttatcccgc tctttcgtcg gtattgtggt ccccttgtag gcctgataag cgtagcgcat
    18361 caggcaatgc ttcctgcttt ataatccctt cagcaacttt tcaacaaact caggcaccac
    18421 ctggcttgcc gggccgtaat atttctcggc aaattcatta ccgacctgac ttggttcgag
    18481 attcagttcc acggtgtgcg cgccatgcag tttcgcttcg tgaacaaacc cagccgccgg
    18541 ataaacatgc ccggaagtac caatggcaat gaaaatatca gccatcgaca acgccatata
    18601 aatttcatcc atgccgagtg gcatttcgcc aaaccaaacg acgtgcgggc gcaagggtgc
    18661 cgggaactgg caacaatggc atttatcttc tggcgtaacg tctccggtcc agtcgagaac
    18721 ctgaccactt tgcgaacaac gaactttcag caattcccca tgcatatgaa tgacattggt
    18781 attacctgcg cgttcatgca ggttgtctat attctgcgtt accagcaaaa agcgatcgcc
    18841 gagggcgtct tgcagtttag ccagcgcaag atgcgcggcg ttaggctgaa tttctggctg
    18901 ctgcagctgt cgacgacggg cgttataaaa cgcttgcacc agttcaggat cgcgatcgaa
    18961 accttccggc gttgccacat cttcaacccg atgttcttcc cacaggccat ctgcggcgcg
    19021 aaaggtacga atacctgatt ccgcagaaat tcctgcccct gtcagtacga gtactcttgg
    19081 tttttccatt gcttccggca ccactttatc tctgaaaaaa atacgctgac gcaaacgctc
    19141 gcgcaggcgg cgtttatttt tacgaaaacg acttaaccga tgaccccgac gcgacagcat
    19201 agcaacctct gttgtttaat cggttagatg taggaaggcc gcaccacgca ttcctcccgc
    19261 atcaccgtgg cgcgcgcgtt caatgcgcgg aacacgagct acaggtaaga gatgacgagg
    19321 cagcctgtcc gccagttgcg ttgtgattgc cgggaaattc gataagccac caccaatgac
    19381 gaccaggtca gagtcaacga tggtcaggat atttcccaga caaaccgcta ataaatccag
    19441 ataacgctca acgtgcgccc ttgcctgctc atcgccttga tcataaagcg caataatttc
    19501 gggagcctgc aacggttgat gataatagtg ttgccagagc cacgcaaagc cccgacctga
    19561 tagataattt tcgatacaac caatttgacc acagccgcag cggcgtaacg ggaaatccag
    19621 ccccatcatg gttaacgcat caaccggcag acgcatatgg ccaaactcgc cggtaatgta
    19681 gcttttaccg gtaatcggtt taccgttgaa aatcagcccg ccgccaacgc cggtgccgag
    19741 aattaacccc atcaccagtg gatattgcgt aaattcatca tcccaggctt ctgaaagggc
    19801 aaaacagttg gcatcgttat caaggcgtac atcgcgatca agacgtgcgc tcaggtcggc
    19861 acgcagcggt ttaccgctgg cagcggggac attggcggca tacagcgtac catcttctgt
    19921 ttccggcatt cccggaatac cgatgccaac agaaccttta cagccaaaac gttggtcagc
    19981 ttcggctacc agctcgcata ctgcatctaa aaatgcgtca tagctgtcac gcggtgtcgg
    20041 cacccgcttt tcccactgca actgccgacc gctatcaaac acgccaagcg caatttttgt
    20101 tccaccaata tcaaacccgt aatacattgc cgctccttgt tttaatgtac tgcctttact
    20161 ggccgctaag gactcgcgca gggtcaatat tactggcgcg ccgcgccgga taccaacttg
    20221 ccaaaagact cagcaacaat gctgtgacca gtacgtagaa gacgtccagc cagtgcaatt
    20281 ccgatggcag gaagtcaata aaatagatat cgctggagag gaactgatga ccgatcagct
    20341 tttcaatccg ctcaataatc ggggtaagtt gcagtgaaac aactacgccg ataatcacac
    20401 cacacaggct accgaacagc cctgccagca atccatacca gacaaagatg gcgcgaatta
    20461 aaccatcttt cgcccccagc gttcttaata ctgcgatatc gccactcttg tctttcaccg
    20521 ccatcactaa ggtggagacg atgttgaaac aggccacgcc aatcaccagt accatcgcca
    20581 gatacataat ggcgcggatc atctggatat cgcgatacat atagccgtaa gtaccaatcc
    20641 aacttttaat ataaacatag ctgttggtca cttcacccgc atcgcgtacc agcttattgg
    20701 cgttgaaaac atccgtcatt ttaagggcaa tacctgacac gctggaaccc atatcaagat
    20761 attgttgggc atccgccagc gggatcatgg caaaactgtg atcgagttga ccactcaact
    20821 gcaaaatacc ggcaatgtgc aaacgcacac gttttggctg catcagttta tgctcgggat
    20881 tcgagttggg aatcatgatc gacacccaat cgccctgctt cactttcagc gcatccgcca
    20941 cgcctttgcc gatgataatt tgctgttcgc ccgctttaaa attgcgccag gcatccccct
    21001 gaacaaacga gggtaatgcg ctcagacgct gttcctgttg cgggttaacg cccttcacct
    21061 gaattgcgcg cagattggta ccactttcta ccagtccggt gaaattgata tacggcgcag
    21121 cggcggcaat acccggcact ttttgcacgt tatccagtgc ttcctgccag ttagtccatg
    21181 gctgatttac cgcctcaatt tcaccgtgcg gtaccacagc aagaatgcgg ttattcagtt
    21241 cgcgttcaaa gccgttcatc gcgcttaagc cgacgatcaa taccgccacg ccgagggcaa
    21301 tgccaatggt agaaatgaca gagattagcg acaccatgcc gccgcgtcgc cgtccgcgac
    21361 taaaacgcag gccaatcaat aacgataaag gcatcgccat tactccgccc ccatcaggct
    21421 cagttccgcc gtcagacgtc catcacgcat ttccagttgg cggctcatac gtttcgccag
    21481 ttgcaggtcg tgagtaacca ccaggaaggc ggtgccctgc aagcgattca attccccaag
    21541 caactggaag atgctgtcgg cgttacgcgc atcgaggtta ccggtaggct catccgcaag
    21601 taccagacgc ggattattga ccagcgcacg ggcaatcgcc acacgctggc gttcgccgcc
    21661 agaaagttca gatgggcggt gattcgcacg atgctccagc cccaccgctt ttaacatctc
    21721 cagcgcacgg ctgttgattt cagcgggctt tttcttgcca atcagcagtg gcatagccac
    21781 gttttccagg gcagtaaaat ccggcagcag gtggtgaaac tgataaataa agcccagctt
    21841 ctggttgcgc agttctgctt tcgccgccga agacagtttg ctcatcggtt gaccgttaaa
    21901 gatcacatcg ccggaggttg gtgtatccag cccgcccagc aggtgcagca aggtactttt
    21961 accggaacca gagctaccga cgatcgccat catttcacct tcgccgacgc tgaaactgac
    22021 attgtgcagt acatcggttt gcacactgcc ttcctgatag cgtttgcaca ggttgtcgca
    22081 ttgcaacagg atcttattca taacgtaaag cctcagcggg ttgagtggcg gcagcgcgcc
    22141 atgaagggta aagcgtagac agcagcgcga tagccatcgc caccagcgca ataacaatga
    22201 cctgtaaagg ttcgatagcg accggcagcg ccgcgccatc aagcaggacg ccgattatcg
    22261 gcatcagatt atttaactgg ctggcaagca gtgcgccaag cgccgctccg aggatcgcac
    22321 cgataatccc ggcgctggcc ccttgcacca taaagaccat catgatttgt cgcggagtta
    22381 agccttgcgt ttgcaggatc gcgacttcgc cctgcttctc cattaccatc agccctagtg
    22441 aggtaataat gttaaacgcc gcaacggcga caatcaggct cagcagcaag cccatcatat
    22501 ttttttccat gcgtacggcc tggaacagct cgcctttacg gtcacgccag tcctgccatt
    22561 tgctgccttc aggcagtttt tgctgactta aagagtcaac tttcagcggc tcatccagcc
    22621 acaaacgcca gccggtaata ttgcctgccg gataacgcat cagacgcgag gcatcctcaa
    22681 tattcaccag catttcatag ccatcgactt cactgttggc ggcgaaattg ccaatcacat
    22741 tgaacaggcg ctggcttgga atacgcccca tcggcgtgaa ctggctggca gatggtacca
    22801 tcacgcggat ttgatcaccg cgattaacgc ctagctgtga ggcaagttgt tcgccgagga
    22861 tgacattata tttccccggc tcgaggtcag tttgtttcac attgaccaga tacggcgtaa
    22921 gtggatcttt ttgcgccggg tcgataccga gcatcacccc gaccgccacg ctgcgcgcgc
    22981 tttgcagtac cacatcaccg gtagtaatag gtgcgacgcg attaacgccg tccagtttga
    23041 ctgccgtttc cgggagttgc tgcgggttaa gagagccatg ctcagaagag agaattgcct
    23101 gtggcatcag gccaaggatg ttgttttgca gctcgcgctc aaagccgttc atcactgaca
    23161 atactgtgac cagcgccatc accccgaggg taatgccgat ggtagaaagc caggagacga
    23221 aacgaccgaa gcgatccgct gcacgcccac gcatgtaacg caggccaata aatagagcga
    23281 caggttggta catgaaatcc gtctggttgc tgtagcaaag acacgagtat ataagcgaaa
    23341 gcaagatggg taaacgtctg aactgtaagt gatcgtcagg gctaatctga aaagcccccg
    23401 taaatcaatc ggctaaattg ccatgtttta tcatcaatct tgccgcctga tcttaatctg
    23461 aaaaatcatc caatacagac agatacccat tacacctcgg gtcataaccc gcaggatcaa
    23521 ccgcatgtaa attaaaaaag agcgtgttgt cctgatgaaa caaaaagagc tatggattaa
    23581 ccagatcaaa gggttatgta tttgtctggt ggtgatttat cactcggtca ttacctttta
    23641 tccgcatatg accacttttc agcatccgtt atcggaagtc ctgagtaaat gctggatcta
    23701 tttcaatctt taccttgcac cctttcgtat gccggttttt ttctttatat ccggctattt
    23761 gattcgccgc tatatcgaca gcgtaccgtg gggaaattgt ctcgataaac ggatctggaa
    23821 catcttctgg gtgctggcgc tttggggcgt ggtgcagtgg ctggcgttaa gtgcgctgaa
    23881 ccagtggctg gcacctgagc gcgatttaag caatgcctcc aatgccgctt atgccgattc
    23941 caccggtgag ttcctgcacg ggatgatcac cgccagcacc agcttgtggt atctgtatgc
    24001 gttaattgtc tatttcgtgg tatgtaaaat ttttagccgt ctggcgctgc cactattcgc
    24061 cttgtttgta ctgctgagtg tggcggttaa tttcgttccc acgccgtggt ggggaatgaa
    24121 cagtgtgatc cgcaatttgc cttattacag ccttggcgca tggtttggcg caacaataat
    24181 gacctgtgtt aaagaggtgc cgttgcgccg ccatctgctg atggcttctt tgcttaccgt
    24241 tctggcggtc ggtgcctggt tgtttactat ctcgctgctg ttgtcgctgg tatcgattgt
    24301 ggtgatcatg aagctgtttt atcagtacga gcaacgattc ggtatgcgct ccaccagcct
    24361 gctgaatgtg attggctcca acaccattgc tatctacacc acccatcgca ttctggttga
    24421 aatattcagc ttaactctgc ttgcgcaaat gaacgcagca cgctggtcgc cgcaagtcga
    24481 actgacactc ctgctggttt atccctttgt cagtttgttc atctgtactg ttgcgggctt
    24541 gctggtaaga aaactttcac agcgtgcatt cagcgatctg ttgttctccc cgccttcact
    24601 gcccgctgcc gtcagttact ctcgttaaac catcgcccca gccggggcga ttcaatttgc
    24661 taaaccatgt cgattacgga taatgaacag cattgcttat cagagatatg cccccatatg
    24721 ttgaggcata tcctaacgag aatctgacaa ccgttatgcc tgaacaatat cgttatacac
    24781 tgcccgtcaa agcgggtgag cagcgtctgc tgggcgagtt aaccggcgca gcctgtgcaa
    24841 cgctggtagc ggaaattgcc gaacgtcacg ccggtccggt ggtactcatt gcaccagata
    24901 tgcaaaatgc tctgcgtttg catgatgaaa tcagccagtt caccgatcag atggtgatga
    24961 atctggcgga ctgggaaact cttccctacg acagtttttc gcctcatcag gacattatct
    25021 cctcgcgcct ttccaccctt taccagctac cgacgatgca gcgtggcgta ctgattgttc
    25081 cggtgaatac gcttatgcag cgcgtttgcc cacacagttt tctccacggt catgcgctgg
    25141 tgatgaaaaa aggtcagcgc ctgtcacgag atgcattacg aacccaactg gacagcgccg
    25201 gttatcgcca tgttgaccag gtgatggagc acggcgaata cgccacgcgc ggcgcgttgc
    25261 tggatctctt cccgatgggg agtgagctgc cttatcgtct tgatttcttt gatgatgaaa
    25321 tcgacagcct gcgggtgttt gacgtcgaca gccagcgcac gctggaggaa gtagaagcga
    25381 tcaatctgct gcccgcgcac gaatttccga ccgataaagc ggcaattgaa ctgttccgca
    25441 gccagtggcg cgataccttc gaagtgaagc gcgatccgga acatatttac cagcaagtga
    25501 gtaaaggcac attacctgcc gggatcgagt actggcagcc attgttcttc agcgaaccac
    25561 tgccgccgct gttcagttat ttccctgcca ataccttgct cgtgaatact ggcgatctgg
    25621 aaaccagtgc cgaacgtttc caggctgaca cgctggcgcg ttttgagaat cgcggcgtcg
    25681 atccgatgcg cccgctgttg ccaccacaat cgctctggct gcgggtggac gagctcttct
    25741 cagagctgaa aaactggccc cgggtgcagc taaaaactga acatttaccg acaaaagccg
    25801 cgaatgccaa tttaggtttc cagaaactgc cagacctggc cgttcaggca caacaaaaag
    25861 cgccgctgga tgcgctgcgt aagttcctcg agactttcga cggtccggtg gtgttctcgg
    25921 tagaaagtga aggtcgccgt gaagcgctgg gtgaactgct cgcacgaatt aaaattgctc
    25981 cgcaacgcat tatgcgtctt gatgaagcca gcgaccgtgg gcgttatctg atgattggcg
    26041 ctgccgaaca tggttttgtc gataaggtgc gtaatctggc gctgatctgc gaaagcgatc
    26101 tgctcggtga acgtgttgcc cgccgtcgtc aggattctcg ccgcaccatc aaccccgata
    26161 cactgatccg taaccttgcg gagctgcata ttggtcagcc ggtggtccat ctggagcacg
    26221 gtgtcgggcg ctatgccgga atgaccacgc tcgaagcggg cggcattact ggcgagtatt
    26281 tgatgctcac ctatgccaac gacgccaaac tgtatgttcc ggtgtcgtca ctgcatctga
    26341 ttagccgtta cgcaggtggc gcggaagaaa acgccccgct gcataaactt ggcggcgatg
    26401 cgtggtcacg cgcgcggcag aaagcggcgg aaaaagtgcg tgatgtggcg gcggaattgc
    26461 tggatatcta cgcgcaacgc gccgccaaag agggcttcgc gtttaaacac gatcgtgagc
    26521 agtatcagtt gttctgcgac agcttcccgt ttgaaaccac gccggatcag gcgcaggcca
    26581 ttaatgcggt acttagcgac atgtgtcagc cgctggcaat ggatcgtctg gtgtgcggcg
    26641 atgttggctt tggtaaaaca gaagtggcga tgcgcgccgc tttcctggca gtagataacc
    26701 acaagcaggt ggcggtgttg gtgcctacca cccttctcgc gcagcagcat tacgacaact
    26761 tccgcgaccg tttcgccaac tggccggtac gtatcgaaat gatctcccgt ttccgcagcg
    26821 ccaaagagca gacgcaaatc cttgcggaag tggcggaagg gaaaatcgat attctgatcg
    26881 gtacgcacaa actgctgcaa agtgacgtca agtttaaaga tttaggcctg ctgattgtcg
    26941 atgaagaaca ccgcttcggg gtgcgtcata aagagcgcat taaagcgatg cgcgcgaacg
    27001 tggatattct gacgcttact gcaacgccga tcccacgcac gctgaatatg gcaatgagcg
    27061 gaatgcgtga cctgtcgatt atcgccacgc cgcccgcccg tcgtctggca gttaaaacct
    27121 ttgtccgtga gtatgacagc ctggtggtcc gggaggcgat cctgcgtgaa attttgcgcg
    27181 gggggcaggt ttattatctc tacaatgatg tggaaaacat ccagaaagct gccgaacggc
    27241 tggcagaact ggtgccagaa gcgcggatcg ccatcggtca cgggcagatg cgcgagcgcg
    27301 aactggaacg ggtgatgaat gatttccatc atcaacgttt caacgtgctg gtttgtacaa
    27361 ccattatcga aaccgggatc gacatcccga cagccaacac cattatcatt gaacgcgcgg
    27421 atcacttcgg tctggcgcag ctgcaccagt tacgcggtcg cgtcggacgt tcgcatcatc
    27481 aggcatatgc atggttgctg acaccgcatc caaaagcgat gactaccgat gcacaaaaac
    27541 gtcttgaagc gattgcctcg ctggaagatc tcggggcagg ttttgcgctg gcaacgcacg
    27601 atctggagat tcgcggcgcg ggtgaactgc ttggcgaaga acaaagcggc tcaatggaaa
    27661 ccatcggttt ctcgctgtat atggagttgc tggaaaacgc cgtcgatgca ctgaaagccg
    27721 gacgcgagcc gtcgctggaa gatctcacca gccagcaaac agaagtcgag ctgcggatgc
    27781 cgtcgctatt gccagatgat ttcattcctg acgtgaatac gcgtttgtcg ttctataaac
    27841 gtattgccag cgccaaaacg gaaaacgaac tggaagagat caaagtcgag cttatcgatc
    27901 gcttcggcct gctgccagat ccggcgcgta ccctgctgga tattgcccgt ctgcgccagc
    27961 aagcgcagaa actggggatc aggaagctgg aaggtaatga gaaaggcggg gtgatcgaat
    28021 ttgccgagaa gaatcacgtt aatccggcct ggttgattgg tttgctgcaa aaacagccgc
    28081 agcattaccg ccttgatggt ccgacgcgcc tgaaatttat tcaggatttg agtgagcgga
    28141 aaacgcgtat cgaatgggta cgccagttta tgcgtgaact ggaagagaac gcgatcgctt
    28201 aatggtaaat cgggggcgtt tctgcgcccc catctgcaac atttacaaat tctttgcact
    28261 tccctgcact atccgacact gtcaccatcc ataattcagg cttatctgtt tattacaata
    28321 accttatatt tattattgat ttttggtgat gatcaaaacg cgtttttctc gctggctaac
    28381 gttttttacg ttcgccgctg ccgtggcgct accggcaaaa gccaacacct agccgctgcc
    28441 gccagcgagc agtcgtctgg ttggcgaaaa caaatttcat gtggtggaaa atgacggtgg
    28501 ttctctggaa gccatcgcca aaaaatataa cgtcggcttt ctcgctctgt tacaggctaa
    28561 ccccggcgtt gatccttacg taccgcgcgc gggcagcgtg ttaacgatcc cgttgcaaac
    28621 cctacttcca gacgcgccgc gcgaaggcat tgtgatcaac attgcggagc tgcgtctcta
    28681 ttactacccg ccgggtaaaa attcggtaac cgtgtatcca ataggtattg gtcagttagg
    28741 tggtgacacg ctgacaccga caatggtgac caccgtttca gacaaacgtg caaacccaac
    28801 ctggacgcca acggcaaaca tccgcgcccg ttataaagca cagggaattg agttgcctac
    28861 ggtagtgccg gctggaccgg ataacccaat gggccatcat gcgattcgtc tggcggccta
    28921 tggcggcgtt tatttgcttc acggtacgaa cgccgatttc ggcattggca tgcgggtaag
    28981 ttctggctgt attcgtctgc gggatgacga tatcaaaaca ctctttagcc aggtcacccc
    29041 aggcaccaaa gtgaatatca tcaacactcc gataaaagtc tctgccgaac caaacggtgc
    29101 gcgtctggtt gaagtacatc agccgctgtc agagaagatt gatgacgatc cgcagctgct
    29161 gccaattacg ctgaatagcg caatgcaatc atttaaagat gcagcacaaa ctgacgctga
    29221 agtgatgcaa catgtgatgg atgtccgttc cgggatgccg gtggatgtcc gccgtcatca
    29281 agtgagccca caaactctgt aaatcctgga taaaaaaaat ccccgcgaca cggcatgtcg
    29341 cggggaaaaa atcagcagta gcaggcatta atgagggtta atgcttattt ataaattact
    29401 gctgttccat ggagggtatt cggaccggtt acagaagtaa tacggaaaga ttttgcgccc
    29461 atctcatccg ctttttgcgc cagctgctct tccagcgatc ccagatttgt ccccgcgtta
    29521 gcactgatag taccgacttt ttgttggcct tctggcgttg actgaacttc gacagccgca
    29581 aagttggcaa atgacatgga gcttaaaatc gccgcagcga tgagggtttt tacgtttttc
    29641 ataatagtgg ccttatgcag atgaatgacg tcgcaagaaa ttacttaacg atcgataact
    29701 aaatgataga tgtgatctgg atcacataca agatattttt tataacaatc attaattaat
    29761 ttaaaaatct ttaaatttca gttaaataga aagcaattat ttttatggga tctggactgg
    29821 tgaacggagg tgattatttt tataataacc tttcattcac agtcaccagg ttcaacggca
    29881 tcatggcaac tgactcaaca caatgtgtaa aaaaaagccg tggccgccca aaagtgttcg
    29941 acagggatgc cgcgcttgat aaggccatga aattgttctg gcaacacggt tatgaagcga
    30001 cttctcttgc ggacctcgtc gaagcgaccg gagccaaagc gcccacgcta tacgcggaat
    30061 ttaccaacaa agaggggtta tttcgtgccg ttctcgaccg ctatatcgat cgttttgccg
    30121 ctaagcatga agcacagctg ttttgtgaag agaaaagcgt ggagtctgcg ctggctgact
    30181 attttgctgc catcgccaac tgctttacca gcaaagacac cccggcaggc tgcttcatga
    30241 tcaacaactg caccaccctc tccccagatt caggagatat cgccaatacg ttgaaatcac
    30301 gccatgcgat gcaagagcgc actttgcagc agtttttatg tcaacgacaa gcgcgcgggg
    30361 aaatcccgac ccactgtgac gtgacacatc tggcagaatt ccttaattgt attattcagg
    30421 ggatgtcgat cagcgcacgc gaaggtgcat cgctggaaaa actgatgcag attgccggaa
    30481 cgactttgcg tttatggccc gaactggtga aataagtcgc aaaaaaagcc cctcagcctg
    30541 agcgaatgag gggccaaatt gcagagtaca gccttgttat actttgttat tcaaaatcag
    30601 ttgcccgtta ctatctagcg ggatctgcgt acccggatcg cggtccatgc ggattttgcc
    30661 ctgctgatcg ccaatcttat aggtcacatc ataaccgagc attttttctg acttgtcata
    30721 caccgtttta caacgctgtt gcgtagtcgt gtaagtatcg ctttcctgga gagagccctg
    30781 gatctggtta ccggcatatc caccacccag cgcccccaca acagtggcga catctttacc
    30841 acgaccacca ccaaactgat gccctatcac gccgccagca acagcgccga gcaccgaccc
    30901 ggtaatgcga ttttcatcct gcaccggtcg acgatgggtc actgtgacgt tgcgacactc
    30961 ctgacgcggt gttttaaccg tttccttgat tggggttgca gaaacaacct gagcgtattg
    31021 cgggccccgt tcaaacacgt tcagactggc cactgccgct acgcccagcg cagctgcgac
    31081 accaatcccg atacccgcca acattgattt attcacggga acacctcctt ctttgcagtt
    31141 atgccaatca tgcaagcgaa ggagaaaagc agctatcaga cagaagatga aaagcgcggg
    31201 aatggctgga aatacttaag gattcggaga agtctgaagg aatgcctgat gcgacgctta
    31261 acgcgtctta tcaggcctac cgctcgtacc atttgtccag ggcggatgag gcgtttatgc
    31321 cacatccgcc agtgtacgtc gattaatgca acttcaaacg cggacggata acgcggttaa
    31381 tactccccac cagcatcatt aatccggttt taaagtaacc atgcagcgca atctgatgca
    31441 ttcggtatag cgagatatat acaaagcgcg caattcgtcc ttcaatcatc attgagccgc
    31501 gcgtcaggtt acccatcagg ctaccgacag tggagaagtt cgacagcgat accagcgaac
    31561 catgatcttt atactgataa tttttcagcg gtttaccgtt catctgcgcc agaatatttt
    31621 tcattgcgca ggtcgccatc tggtgtgcag cctgagcacg cggcggaaca aagccccctt
    31681 ccggacgcgg gcatgacgcg cagtcgccaa tagcgtaaat gtctggatcg cgggtggttt
    31741 gcagcgtcgg ttccaccacc agctggttga tacggttggt ttcaagacca ccgatatctt
    31801 tcaggaagtc tggcgctttg atcccggctg cccacaccat cagatcagcc tcaatatatt
    31861 caccatcttt agtgtgcagg ccgccttcat cagcactggt gaccatggtt tgcgtcagca
    31921 cgcgaacgcc aagtttcgtt agctcgttgt gggccgcagc agagatacgt ggcggtaatg
    31981 caggcaaaat acgttctccc gcttctacca gcgttacgtt cagggcttcg ttggtcaggc
    32041 ctttgtaacc gtagctgtgc agttgcttga ccgcattgta caattcagcg gagagttcta
    32101 cacccgtcgc gccgccgccg acaatcgcaa tgttcacttt accattcgcg cccaggttgg
    32161 cggagtattt caggaacaga ttcagcatct cctggtggaa gcgacgcgcc tggtgcgggt
    32221 tatcgaggaa aatgcagttc tctttgacac ctggcgtatt gaaatcgtta gaggtgctac
    32281 ccagcgccat taccagggtg tcataggcga ttttacgttc cggaaccagc agttcacctt
    32341 tctcatcgcg cagttctgca atagtgattg ttttcgcttc acgatcaata tcaatgacgg
    32401 aacccagctg gaactggaaa ccatgattgc gcgcatgggc cagatagctc aacgcatcga
    32461 cgccttcatc aagcgagcca gtcgccactt cgtgcagcag cggtttccac agatggctgt
    32521 ggttacgatc gaccagcgta attttggctt ttttcttgcg tcccagctta tgccccagct
    32581 gtgttgccat ttccagccca ccagcaccgc cgccgacaat cacaattttt ttcaatggcg
    32641 tagtcaacgt gaccccctta aatttattaa ccaattgtta attaatggtt ataaacatag
    32701 ctcttaatta acaacaggtt acagcaatga aaatgttctt cgctattgag aataacatga
    32761 atggtgcatt ggtcatacca aaattgatgt taatcaagtt ttgttgctga aaagataggc
    32821 gactttaccg ggtgagatca aaaaacagag tttattgttt attatttgag caagtccgga
    32881 agggtgatat agggtgacgc gtatatcgag ggcgtttggt cgtcctgata agatgcgcag
    32941 catcgcatca ggcattgatg ctcgactgtc ggatgcggcg taaacgcctt atccgactta
    33001 cagaattcgg agcgcatcag gtaccggcaa ggttacctga tgcgacgagg gcatttaccc
    33061 gagggttttg aacgccttaa tgcgctgcaa atgcggggag atattcttga atttgtgcgt
    33121 ctgctcttcg tcccagacaa tctcgtaata atgatgcaac tcttcagatg tccgctggct
    33181 gttaagcgct tcatcattac gcgacaaaat caccaggcaa cgatcgcgat tcttctcgcg
    33241 gaagttggtc acacacttag tggcaatatc ggcatactct tccgggcgat caatcttgcc
    33301 ttccatgttc tcataaggga acaaattagg gttgaagatc acctggcgga tatcgcaaag
    33361 aaaaccaatc cgttccgccc agtatccgcc cagaccaacg ccgcaaatca gcggacgctc
    33421 gtcgacgttc agttgcaaca ttttgtccac ttctttgagc agatgctgca tatcatgttt
    33481 cggatgccgc gtactgtagc ttatcaagcg tacatccggg tcaataaact gcaattgtaa
    33541 gactttctcg tggttacccg gactgttaga gtcaaaaccg tgtaaataga taatcatcgc
    33601 ttcctcacat aagccagggt tagtgacctg ctttctcttc ctgccagcgt tcatgtaact
    33661 gattcagacg ggtgctgatc gccttccagc gagccgagtc catcagttcc tgtcgcgaaa
    33721 atgaaccttt atgatacaaa cgtgtaacac gttctgcctt gatcggtgac agattatcta
    33781 acacgctgac ggccccttta cgattattgc agaccaggat catatcgcaa cctgcgtcca
    33841 gtgatgcctg cccgcgttcg gcataactgc ccataatcgc ggcaccttcc atcgataaat
    33901 cgtcagagaa aattacaccg tcaaaacaca gttcctgacg caaaacggtt ttcagccagt
    33961 aggaagaacc gctcgccgga cgcggatcaa catcactgta gatcacatgc gcaggcataa
    34021 tggcgtcgag tttattttcg cggattaagg aactgaagac cgacatatct ttagcgcgaa
    34081 tctccgcttg tggacgtgga tcgcacggtg tttctttgtg tgagtctgcc gttactgcac
    34141 cgtgtcctgg gaagtgtttc ccggtcgttt tcattccggc ttcatgcata ccatcaataa
    34201 accggctggc aattgccagg gctttttgtg gatcggcatg ataagaacgc tcgccaatcg
    34261 ccgcgctgat atgcccgaca tccagcactg gcgcaaagct gatatcaata tccatagcga
    34321 tcatttcgct ggccatcagc caacccgcct cttgcgccag tttgccgccc tcttccattc
    34381 ccaacagcgc agcaaaggat tgtgctgccg gtaagcgggt aaaaccttcg cgaaaacgct
    34441 gcacgcgtcc accttcctga tctaccgcca ccaccagatg attgcgcgat gctgcgcgga
    34501 tctggcgcac cagttcacgt aactgggcag gatcatgata gttacgcgta aagagaatca
    34561 gccctcccac cagcggatgc gccagtattt cacgctcttc cgcgtccagt tcgtaacctt
    34621 cgacatccaa cattactgga cccacactgc tctccttatt gttttattaa tagctgccgc
    34681 caggtgtcat cggccagcct gataaattgt tgatcgccga tttgtcgcca gcggtactca
    34741 aaccaccctg ctttgagcat cagcagccag ggaaaccatc gcctgacctg acgccataat
    34801 tgcgccggat aaatcttcgc gcgagtggca tagtcattga ccaattgccg gtgctgttca
    34861 gtattttcca cccacaccgc cgccagttcc agcgcgatat caccatctcc ggcatactcc
    34921 cagtcgatga gttttaaccc tgacgcgcta tgcactaaat ttccggcgtg gacatccata
    34981 tgcaatggac ttaagcgtaa aggccgtggt tcccgcgctt tgcgcagacg ttttaacatt
    35041 cgcagccaac ccacagtccg ccgcgccgga tcgctttgct gccagtacag ttccagtaag
    35101 ggcaacagcg ttattcgcca gccaaaacgt ggttgttgat gaagataata cagcaagcct
    35161 gccagttcgt tggtatccgg caaatacgtt tttaccgcgc cgggcagata gtcgactacc
    35221 atccagtcac ggagatataa atgcggcttc ggtgcaatgc ttgcgggtag ttgtgataaa
    35281 gcccgatact ggcgcaagaa cgcggactgc ggcgcatcag gatcgtgcgg ctgacgcaca
    35341 acaaaacgct ggccctgatg ttcaatgaga aaactaccgc cactaagccc actattaaac
    35401 gtcgtgacgg gatgaaactg cgggaaaaag cgcgacagca attcgtcgcg cgtgatggga
    35461 ttattgctgc gaaacggcac ctttacctga ccagataatt tcgcccgtct gcaccagcat
    35521 cagctgcatt tgtagggtcg gagccttaac gttgccagag gcgctggagt acagcacgta
    35581 atgagcgccg acattgcggg caatgcctat ggctttacta cgggtaccta aactgtcctg
    35641 cggcgacaaa cctaactgtt gcttagccat cgacagctgc tgggcggaaa ccagggtaaa
    35701 tttcccgtta ttagccagcg catttcgcag cgtttcggtc gcttctgcgg cattcagcga
    35761 accgttagta cggttgttaa cgctatcaac cagcaggacg ctacccgcag tcaccccgtc
    35821 agccccaagc atcttactga ccatcggctg cattgcgcca ttccagtcat aatggcgaat
    35881 atgcggcgca ggcggtgccg tttgatcttc gtgctcaatt gggcctggct gctgcgggat
    35941 cgtcggcacc gagggcactg tggggacagg ctgttgtggc tcggctggtt gttccggcgc
    36001 tggtttcact tcttctaccg gtgcaggttc acgttgcccc acacacccgg cgagaaacat
    36061 cgccagcgcg gtaatcaagg cgtagcgact catttttgtc atcaagattc accccttaca
    36121 aatatagata aagtctgact ttgtgcgccc ccagaaaatt ggcgctgccg tacagcgtta
    36181 ccgccgaatg tgcgggaatg ctaacgctgc gtggcctttc cagaggatgc atctccagcc
    36241 ctctggcgtc ataccagtaa aaacgataat gaacggtaac gggttcttgc ctttcgttat
    36301 aaagcgttga ggatgctgaa ggttgaatat cagacgtcga aaggacgggc ttttctgcac
    36361 tgatgcccgc agccaataaa gatgactcca tcaccagcga ttgctcatca ttcaccggaa
    36421 tttccggatg tgaacgacag cccaccagca gtaacaacgc cagagacacc agcccaaagc
    36481 atccttttct catcgttaca gacctttatg cgccagcatt ggtcccagcg gacggccacc
    36541 caacaagtgc atatggatgt ggtaaacctc ttgtccgcca tggcggttgg tattcatgat
    36601 cagacgatag ccatcttcgg caataccttc ttgctcagca atttttgccg ctacggtgat
    36661 catgcgtccc agcgcctgct catgctcagc tgacacgtcg ttcacagtcg gaatgaggat
    36721 attcggaatg atcagaatat gcgttggcgc ttgcggcgaa atatcgcgaa acgccgttac
    36781 cagatcatcc tggtagacga tatcggaggg gatctcacga cgaataattt tgctgaatat
    36841 agtttcttct gccacgacat tttccttttt caaatagcca tgcgttgcgc catgctacgc
    36901 cgcgccacac tgcgagtata gagtatggtc gagttaaacc tgctctttca accttaactc
    36961 acgatttctt aagcaaaaaa tcgttttgct gccgtatata tcgccattat tcccatttct
    37021 gcctgcgaat gcgctgaaaa gacattctct gtttggttta cacgacaaat atgaatgcgc
    37081 atatttctca tttgcattta caaacaaaat tattcgcaca ctaaatataa ccgctcatca
    37141 ccctcctccg gcatgagcct gacgacaaca taaaccaaga gatttcaaat gctttcaaca
    37201 caatttaaca gggataatca atatcaagcc atcaccaaac cgtcactact tgccggttgc
    37261 atagcactgg cactattacc ttctgccgct tttgctgcac cagccactga agaaacggtg
    37321 attgttgagg gttcagccac agctccagat gatggcgaaa atgattacag cgtaacgtct
    37381 acctctgcgg gtaccaaaat gcagatgact caacttgata ttcctcagtc ggtcactatt
    37441 gttagccagc agcggatgga agatcagcag ttacaaacgc tgggcgaagt gatggaaaac
    37501 acgctgggga tcagcaaaag tcaggcggat tccgatcgtg ctctttatta ttcccgtgga
    37561 ttccagatcg ataactatat ggttgatggt atccccacct attttgaatc gcgctggaat
    37621 ctgggcgacg cactttctga tatggcactc tttgaacgcg tagaagtagt gcgtggcgcg
    37681 acaggactca tgaccgggac gggtaatcca tctgcggcaa ttaatatggt tcgaaaacac
    37741 gcgaccagtc gtgaatttaa aggcgatgtc tcggcggaat acggtagctg gaacaaagaa
    37801 cggtatgtgg cggatttaca aagcccactc accgaagacg gtaaaatccg cgcgcgaatt
    37861 gtcggcggct accagaataa cgactcatgg ctggaccgct acaacagtga aaagaccttc
    37921 ttctcgggca ttgtcgatgc tgatttaggc gatcttacga cgctttcagc cggttacgaa
    37981 tatcagcgca ttgatgttaa tagcccaacc tggggcggtt taccgcgctg gaatactgat
    38041 ggcagcagca acagttacga tcgtgcacgc agtaccgcac ctgactgggc gtacaacgat
    38101 aaagagatca acaaggtctt tatgaccctg aagcagcggt ttgctgatac ctggcaagcg
    38161 acactgaatg ccacccactc tgaagtcgaa tttgacagca aaatgatgta tgtcgatgcc
    38221 tatgtaaaca aagcggatgg tatgctggtt gggccataca gtaattatgg acctggcttt
    38281 gattatgtcg gcggcaccgg ttggaacagt ggcaaacgta aagttgatgc gctggatttg
    38341 ttcgctgacg gtagttatga attgtttggt cgtcagcaca atctaatgtt tggtggcagt
    38401 tacagcaaac aaaacaatcg ttacttcagt tcatgggcca acatcttccc ggatgaaatt
    38461 ggcagtttct acaactttaa tggcaatttc ccacaaaccg actggtcacc acagagcctg
    38521 gcacaggacg ataccacaca tatgaaatcg ttatatgctg ctactcgagt cacccttgcc
    38581 gatccgctgc atctgatcct cggcgcacgt tataccaact ggcgggttga tacgctgact
    38641 tacagcatgg agaaaaacca caccacgcct tacgctggtc tggtgtttga catcaatgac
    38701 aactggtcga cctacgccag ctatacctct attttccagc cgcaaaatga tcgtgacagt
    38761 tcaggcaaat atctggctcc aatcaccggt aacaactacg agctgggtct gaaatcggac
    38821 tggatgaata gccgtctgac caccacgtta gccatcttcc gtattgagca ggataatgtc
    38881 gctcagtcca ccggtacacc tatccccggc agcaacggcg aaaccgccta taaagcggtg
    38941 gatgggacag tcagtaaagg ggtggaattt gaactcaacg gcgcaattac cgacaactgg
    39001 cagctgacat ttggcgcaac gcgctatatt gcagaggata acgaaggaaa cgccgttaat
    39061 cctaatctgc cacgcaccac ggttaaaatg ttcaccagct atcggttgcc tgtcatgcca
    39121 gagttgacag tcggcggtgg tgttaactgg caaaatcgcg tgtataccga caccgtgaca
    39181 ccgtatggca ccttccgcgc cgagcaaggt agctacgcgc tggtggatct cttcacccgc
    39241 taccaggtga cgaaaaactt ctcgttacag gggaacgtca ataacctgtt cgacaaaacc
    39301 tacgatacca acatggaagg ttctatcgtc tacggcgcac cgcgtaattt cagcattacc
    39361 ggcacgtatc aattctgaat aacacctgta aaaaaggcag ccatctggcg gccttagtct
    39421 ccccaacgtc ttacggatta gtggttacgg atgtactcat ccatctcggt tttcaggtta
    39481 tcggatttag taccgaaaat cgcctgaaca ccagaaccag caaccactac gcccgctgcg
    39541 cccagtttct tcaggccagc ctgatccact ttagacacat cagcaacgct gacgcgcaga
    39601 cgggtaatac atgcgtcgag gttagtaatg ttttctttac caccaaatgc agcaaccaga
    39661 gccggtgcca tttcgctggt acctgttgct tttgcatctt cagtcgcgtc ttcacgaccc
    39721 ggcgttttca gatccagtgc tttaatcagc acgcggaaga tggtgtagta aacaatcgca
    39781 taaccgatac cgacgatcgg gaacagccac agtttgctgc tgttaccaga cagaacgata
    39841 aagtcgatca gaccgtgcga gaacgacgta ccgtcacgca tccccagaag aatacagatt
    39901 gggaatgcca ggcctgccag aatcgcgtgg atgatgtaca ggatcggcgc aacgaacatg
    39961 aaggagaact cgatcggctc ggtgataccg gtcaggaacg aggtcagcgc cgcggagatc
    40021 ataataccgc ccactttcgc gcggttttct ggtttagcag agtgccagat agcaattgcg
    40081 gcagctggca gaccgtacat tttgaacagg aagccaccag acagtttacc cgcagtcggg
    40141 tcacccgcca tataacgcgg aatgtcgccg tggaaaacct gacctgctgc gttggtgtat
    40201 tcaccaatct gcatctggaa aggtacgttc cagatgtggt gcagaccaaa cggcaccagg
    40261 caacgttcga tgaaaccgta aatgccaaac gcaactaccg ggttctggta agcagcccac
    40321 tgagagaagg tctggattgc agaaccaatc ggcggccaaa tgaaggacag cacaacgcca
    40381 gtaaagatgg cagccaggcc agaaatgatc ggcacaaagc gtttaccggc aaagaagcca
    40441 agatactcag gcagcttaat acggtagaaa cggttaaaca tgtacgctgc gatcgcaccg
    40501 gagataatcc ctccgagtac gccagtatcc gccaggtgtt tagaggcgat ttcttcagca
    40561 ggtaaatgca gtaccagtgg cgcaaccacg gccatggttt taaccatgat gccataggca
    40621 acaactgcgg ccagcgcgga tacgccatcg ttattggtaa agccgagggc gacaccgatc
    40681 gcaaaaatca gtggcatgtt tgcaaagacg gaaccgcctg cttctgccat aacatgcgat
    40741 acaacggcgg gcagccagct gaaattcgcg gaaccgacgc ccagcagaat acctgcgata
    40801 ggcagtacgg ataccggcag catcagcgat ttaccgacct tttgcaggtt agcaaatgca
    40861 ttcttaaaca taattgagag tgctcctgag tatgggtgct ttttttacgt tctcacgcgt
    40921 ggcaaggggg gagagcctcg ccgtgtacag ggcatctaag cgccctttat ttattacaca
    40981 gagtaaaata attcagtgcc gatttgtttg acggctatca catttcaact tcagatcatt
    41041 ggagaagttg cacattttga caaaacatat gtcacaatat ttcaaacgtg gttattcacc
    41101 gcccaatttt cggcggtgaa ctttactcgt tttagccatt aattacgagc tttaaaaaaa
    41161 ctcattcaac ggatggattg aaggcgggaa gcgtcgatgt gaaacagacg ggcgaagtta
    41221 tcggtggtta cctgcgccag ttcttcaacg gcaacacctt tcaacacagc catgtattct
    41281 gcaacgtcac gaaccatcgc aggttgattc tcttttcctc gatgcggtac cggcgcaagg
    41341 taaggtgagt cagtttccac cagtaaccga tccaggggga cataacgcgc agcatctcga
    41401 agttgctccg cattacggaa ggtcacaatg ccggaaaagg agatgtaaaa tccgagatcc
    41461 agtaatttac ccgccgtttc tctgtcctct gtaaaacagt gtagtacgcc accgcaatcc
    41521 gtcacttttt cttcgcgcag aattgccagc gtatcggcgc gggcgtcacg ggtatggacg
    41581 ataaccggct tgttcagttc acgaccaatc tggatatgat ggatgaagga ctcttgctga
    41641 cgaactttag tttccggtgt gtaataataa tccagcccgg tttcacccag cgctacaaca
    41701 ccctcttctg ccgccagacg gcgtaaatct tctacatcgt agggatcatt ctggttgagt
    41761 ggatgcacgc cacaagaaaa tacgacgttg tcacgttcgc ctaccagatc ccgcatatgt
    41821 aaataacccg gtaatgttgt ggcgactgcc agacaaaatt tcacatcgcg tgcggcggct
    41881 ttcgccagaa cgtcatccac gtccttatgc aaagattcat aatccagacc atcgagatgg
    41941 cagtgtgagt cgactaaaaa catgatgtct ctcttaaaga tgaggaaccg gtagcacaac
    42001 gcccggttgc aggtaatgct caatacgcag taaaagatcg gtgatgagaa gctcgcggtt
    42061 gatgcctgtg acagacatta actgttcacg aatgtggcaa acatccccca gtatagcctg
    42121 caggcgcgag ggagaaagat ggtttgccag ttcgacgaca aggcccggca catcaacatt
    42181 ggtcacctgc gcagcaccat gatggcgttt tagcgcatcc atcagcaacg ttgccagcca
    42241 gtgtaaacgc gccggagctt gttcatgatt aagggccgcc agcagcgaat accagtcgcc
    42301 cgatggcacg ctatatgcca atgcctgaca caatgtttca cgagcctgcc agttatctcc
    42361 ctgaaacaac gccagtgccg cgccaggcga accggcgctt aagcgcaatg cggcaagtaa
    42421 tgcatcctgt gacattgtca cttcgcgtga aagccaggtc acggcgtact gttccggcgg
    42481 cggcgcaagg taatgtaacc gacaacgact acgtaatgtt gccagtaaac gttcaggctc
    42541 gcgggtagcc aggaaaaacc aggtttctgc tggtggctct tcaagcgttt tcagcaatgc
    42601 gttagccgcg gcgtcggtca gtaaggcagc atcggttacc cagacaactt tcgcaccacc
    42661 taggcgtgcg tgctcattca gcttttcggt gacctcacgt actgcatcaa tgcccagcgt
    42721 atttttccct ttttcgggag ccagggtgta gtaatcggga tgcgtgccag cctgcatcaa
    42781 ctgacatcca cgacagtgac cgcaactttt gtggccctgc ggttgttggc agagtaaata
    42841 acggctcagg gcgtagatta aagcatcatc gcccatgccc ggtaacgcct gaatgagtag
    42901 cgcatggtga cctcttccgg cctgatagct ggctaccagt ttttcgaaat caggtcgtaa
    42961 ccatggatac catctcatgc gtccaactcc ttcacccagt gggtcacggt agtgcggatt
    43021 gcatccatca cggcctccag cggctgggtg gcatcaatgg tatgaatact tttatcttgt
    43081 gcagccagtt ccagatagcg ggcgcgggtg cgattaaaga aatcgaaaga ctcttgctca
    43141 atacgatcca gctcgccgcg cgcacgcgcg cgttttaagc caacttccgg ggtaacatcg
    43201 agatagagcg ttaagtcggg gcgaaaatcc ccgagaacag catcacgcag tgttgccagc
    43261 atatgttggt caataccacg tcctccgccc tgatacgcct gagtggagag atcgtggcga
    43321 tcgccaatca cccaggtgcc gttagccaga gctggtttga tgaccgtttc taccagttga
    43381 acgcgcgcgg cataaaacat cagaacttcg gctttatcgg taatgacttc atcgcctacc
    43441 gatttgatat ccagcaccag gcttcttaac ttttcggcaa gttgcgtacc gccaggttcc
    43501 cgagtgaaaa ccatgtcgcg gatacccagt tgctcaagcg tctcaaccac cacattacgc
    43561 gcggtagttt tgcctgcgcc ttccagcccc tcaatgacga tatacttact gcgcattttt
    43621 ttccttaagc actttcagat aatcctgcac agacttgtta tggctggcaa gattggtatt
    43681 aaacgtgtga ccacctttac catcggccac aaaatagaga tacggcgttt ttgccggatg
    43741 cgcagcagcc ttcagcgaat ccgcccccgg cgtagctatc gcacccggcg gcaaaccggt
    43801 aatggtatag gtgttatacg ctgtcggcgt ttccaggtct gcacgagaaa gtttgccatt
    43861 ataacgctct cccatcccgt aaatcacggt tgggtcggtc tgcaggcgca taccaatgcg
    43921 taaacggttg ataaataccg aggcaacctg atcgcgttca ctggcaacgg cggtttcttt
    43981 ttcgataatt gatgccatcg tcaccagctg atttttatct ttataaggca gaccgtccgc
    44041 acgcccttcc caggcgctat cgaccgcttt caccattttc ttgtgcgctc gcttgagtaa
    44101 cgcgacatcg gtggtattgg cggtatacat ccaggtgtct ggccagaacc aaccttcaat
    44161 ccactccggg ttttccagtt caagtgcctg cgctacggtg gcgtacttat cgtcactcag
    44221 cgtgtgcttg atatacggag cctcacgcaa ttgcttgagg tagtcgctca gacgcatccc
    44281 ttctaccagt cgcagcggga actgtgcttc tttaccgctt tccagcaatt tcagcatctc
    44341 gcgcacggtc atctgcggtg taaagcggta agtcccggct ttaaagtgag aaagatccgg
    44401 ttcgatacgc agcaaccatt gaaaaacccg tgggcgattg atgatcttat cggcataaag
    44461 ctgttcacca agcgccagac gtccggtccc tggcttcagg gtaaatatcg tctcttcttt
    44521 gataagcaat ttgctgtcgg caagatggcg aaccttccag acgcccacac cagcggcgat
    44581 acccagtacc accagcaata acaagataat caataacact tttttcatga ctaattcggg
    44641 cgctcacaaa gtggggctaa atattcatat aacgttgctg acgaaaagga gacatcgcca
    44701 caggcacgta cgggcatcac tggcattaac gcattacaaa taaccatctc atctgcctgc
    44761 aacgcctctt ccagagaggc ttgcacttcg acaagctgat aagaggattg tgccagcaaa
    44821 cggatacaga attgtcgcat aatgccgtta acacctgcct gatccagacg cggcgtataa
    44881 actacattgc ccttccgcca gaacaaatta gccgcacagc attccgtaac ccacccttcg
    44941 ctgtcaagga ccagcgcctc atcagcgttt gtctgctcaa gatgagagcg gatcaatact
    45001 tgctcaagcc gattaagatg tttaatacct gcaagatgag gattgcgccc cagccgcacc
    45061 gggcttagcg ccaacgtcat cccttcgtta cgcaaacggt cgtaatgtgc aggataaacc
    45121 gtaacggaga gaatccgcgt tgccggtcca ctgttcaatg tgctgtaccc tcgcccgcca
    45181 ctaccgcgac tgatcacgac tttcagcaca ccattttgct gttctgctgc cagcgttttc
    45241 atctcttgtt caagctgagg ccagaagtca cagaaaatca tcaaccgctg acaagcatcc
    45301 tgtagtcgct ggatatgcgc cgataacaaa ctgactttac cgtcgataac tctggcggtg
    45361 gtaaaacaac catcaccaaa ctgcgttgcc cgatcgctta ctgccagcga ttcctgctta
    45421 tgaccgttaa ttaagaacat accggctcct tatcagagaa ttgttagtgt ggcagcatgt
    45481 tcactacgga acaagtcgga ataaaagcta agaaaaaagg cccgcaagcg gaccttttat
    45541 atgggtggga aatgacaact tagatctttt taaagatcaa agaaccatta gtgccaccga
    45601 agccgaagga gttacacagc gtgtattcca ttccgctgac ctgacgtgct tcatgcggta
    45661 cgaaatccag atcgcaacct tcatccgggt tatccaggtt gatggttggc ggaacagcct
    45721 gatcgcgcag cgccaggatg gagtagatag attctactgc acccgccgca cctaacaggt
    45781 gaccggtcat agatttcgtg gagcttacca acacacggct tgcagcttca ccgaagatgg
    45841 ttttcaccgc ctgcgcttca gctttatcgc cagccggcgt agaagtaccg tgcgcgttaa
    45901 cgtagccaat ctgactcgct tcaatgcctg catcacgcag agcatttgcc atcgccagag
    45961 ctgcgcctgc gccattttct ggcggtgacg tcatatgata agcatcgctg ctcataccaa
    46021 agccgacgag ttcagcgtaa attttcgcac cgcgtttttt cgcgtgttcg tactcttcaa
    46081 gtaccagcat accggcacca tcgcccagta cgaagccatc acgctcttta tcccacgggc
    46141 ggctcgccgc ttgcgggtta tcattgcggg tagataatgc acgtgccgcg ccaaaaccac
    46201 caacacccag cggcgtactg gctttctctg cgccacctgc aaccatcacg tcagcatcgc
    46261 catacgcgat aatacgcgca gcatggccaa tgttgtgcac gccggaagta caggcggtcg
    46321 cgatagagat gcttgggcca cgcaggccat acatgatagt cagatgacct gccaccatgt
    46381 tcacaatcgt tgacggaacg aagaatgggc tgatcttacg tggaccaccg ttcatcagag
    46441 atgtgtggtt ttcttcgatc agtccgaggc cgccaatccc ggagccaatt gcggcaccaa
    46501 tgcgggttgc gttctcttcc gttatatcaa ggccagaatc ctgcatggcc tgaacgccag
    46561 cgacaattcc atattgaatg aaggcatcca tcttgcgctg ttctttgcgc gagataatgt
    46621 cctcacagtt aaaatccttt actaagccag caaatttcgt tgcataggcg ctagtatcga
    46681 aatggtcgat taggctgatg ccactctgac cggcaagcag agctttccag gtagactcta
    46741 cggtattgcc gacaggagac aacatgccca gtccggtcac aactacacga cgcttagaca
    46801 cgtttgtcct ccagggaggg aaaaaatgat tctagtggga caaaaagata aaactcaggc
    46861 ggtcgaacga ccgcctggag atgttcactt acgcctggtg gccgttgatg taatcaatgg
    46921 cagcctgaac ggtggtgatt ttctcagctt cttcgtccgg aatctcagta tcaaactctt
    46981 cttccagagc cattaccagc tcaacggtgt caagagaatc cgcgcccagg tcttcaacga
    47041 aagaagcatt gttggtaact tcttcctgct taacgcccag ctgttcgccg ataattttct
    47101 taacgcgttc ttcgatagtg ctcatactct taaatttcct atcaaaactc gctttcgcga
    47161 tggttttcgt agtgtataaa atgttgaaaa atttgcaact aaatcccggc aggtcttacc
    47221 acgattttac gttattttga ggccttaccc cccaataacg caaatcattt tgcactaatt
    47281 attactcata accacgcaga ctgcgcgcaa catgagcatt ttgtgcaaat cgcggtcaga
    47341 ccatgtacat cccgccgttc acatgcaaag tttcacccgt gatgtaagct gcttcgtcgg
    47401 atgccaggaa tgcaaccgcg ttggcgattt cctgtgcgcc gccgaggcga cccgcaggaa
    47461 cctgcgccag gatacccgca cgctggtcat cgctcagcgc acgtgtcatg tccgtttcaa
    47521 taaagcccgg agcaacaacg tttacagtaa taccgcgtga cgcaacttcg cgcgccagtg
    47581 atttactgaa gccgatcaag cccgctttcg ccgcagcgta gttggcctga ccgccatttc
    47641 ccatggtacc aaccacagaa ccgatagtga taatacgacc atgacgcttt ttcatcatag
    47701 cgcgcattac cgcttttgac agacggaaaa cagatgaaag gttggtttcg ataatatcgt
    47761 tccactcttc atctttcatt cgcattaaca ggttatcacg agtgataccg gcattattga
    47821 ccaggatatc cacttcacca aattctgcgc gaattttttc cagaacagat tcgatagatg
    47881 ccgggtcggt cacattcaac atcagacctt tgccgttggc acctaaataa tcgctgatcg
    47941 cctgagcgcc attttcactg gtcgcagtgc caataacttt cgcgccacgg gctgcgagcg
    48001 tttcagcaat tgcgcggcca attccgcggc ttgcaccggt taccagtgcg atttttcctt
    48061 caaaattcat gattttcctc ttttaaagct caagcgccgc tgccatcgct gaaggttcgt
    48121 tcagcgccga ggcggtcagg gtgtcgacaa tgcgtttcgt caggccagta agcaccttgc
    48181 ccgggccgac ttcatagaga tgttctacgc cttgcgctgc catgtactca acagacttcg
    48241 tccactgaac cgggttatac aactgacgta ccagtgcgtc acggatggca tcaccattgg
    48301 tttcgcattt cacatcaacg ttattcacaa cagaaactgt tggtgcgtta aaggtgattt
    48361 tcgctaattc tactgccagt ttgtcggctg ctggtttcat cagcgcacag tgagacggta
    48421 cgctcactgg taacggcaga gcacgttttg cgcccgcagc tttacaggca gcgccagcac
    48481 gctcaaccgc ttctttatga ccggcaataa ccacctgtcc cggagagtta aagtttaccg
    48541 gagaaacgac ctgacctgct gcagcttctt cacacgcttt cgcaatagac gcatcatcca
    48601 gaccgatgat tgccgccata gcgcccgtac cttccggtac ggcttcttgc atgagcttgc
    48661 cgcgcatctc aaccagacgc accgcatcag cgaaatcaat cacaccagcg caaaccagcg
    48721 cggaatattc ccccaggctg tgaccggcca tcattgccgg tgctttaccg ccctgctgct
    48781 gccatacgcg gtacagcgca acagaggcag tcaacagcgc tggctgggtt tgccaggttt
    48841 tattcagttc ttcagctggc ccctgctggg tcagcgccca caggtcgtag cccagcgccg
    48901 cagaagcttc agcaaacgtt tcttcgacaa ttggatagct cgccgccata tcagccagca
    48961 ttccaacggt ttgagaaccc tgtccaggga acacaaatgc aaattgcgtc atgttttaat
    49021 ccttatccta gaaacgaacc agcgcggagc cccaggtgaa tccaccgcca aaggcttcaa
    49081 gcagaaccaa ctgccccggc ttaatgcgcc cgtcgcgtac agcttcatcc agcgcgcacg
    49141 ggacagaggc cgcagaggta ttaccgtggc gatccagcgt caccacgaca ttgtccatag
    49201 acataccgag ttttttcgcc gttgcactga taatacgcag gttagcctga tgcggaacca
    49261 gccagtccag ttgagaacgg tcaagattat ttgccgccag cgtctcatca acgatgtgcg
    49321 ccagttccgt taccgcaacc ttgaagactt cgttgcccgc catcgtcaga tgaattgaat
    49381 tctctggatt cacacggtca gcattaggca gcgtcagcaa ctcaccatag ctaccgtcgg
    49441 catgcagatg ggtggagatg attcccggct cttcagaggc agccagcacc gcagcgcccg
    49501 cgccatcgcc aaaaataata atagtcccac gatcggttgg atcgcaggtg cgcgccagta
    49561 catcggaacc gacgaccaga gcatacttca ccgccccaga tttcacgtat tgatcggcta
    49621 cgcttaatgc ataggtgaaa cctgcgcagg ctgctgcaac gtcaaatgcc gggcaacctt
    49681 taatgcccag catgctttga atctgacaag ctgcgctcgg gaaagcgtgc gtagcagaag
    49741 tcgttgccac aacgatcagg ccaatctggt ctttctcaat gcccgccatc tcaattgcgc
    49801 gtgtcgccgc ttcaaagccc atggttgaaa cggtttcgtt tggcgcggca atgtggcgtt
    49861 cgcggatacc ggtacgagtg acaatccact cgtcagaggt gtccaccatt ttttccaaat
    49921 cggcgtttgt ccgcacttgt tcgggcagat agctgccagt accaataatc ttcgtataca
    49981 tgtacgctca gtcacttttc ggttatatac cgtcacttgc aaactgcgag ttcgctggca
    50041 gcgtcctgct accgcagagt tccgcttttg ccaccgtcca gcagctcaaa accagctggg
    50101 tatacagatt ccaggcgagc ggcaattcgc tgaggaactt gtcgctgcac cgcctgcact
    50161 gcctgttcaa tcgcgaccgc aaaagctcgc tgattggctg caccatgact ttttatcacc
    50221 gtgccgcgca atcctaacag acaggcgccg ttatactggt cggggttgag gtgactgaat
    50281 cgcctcgtca ggctcttttg tagccaacgc tttaataaca gtagccacca cgaccgtttt
    50341 ttcccttcac cctgagattt cagcagagaa aggaacatcc tgacaacacc ttccatcgtc
    50401 tttaatgtga catttcctgt aaagccgtca caaaccagca catctgtctt gccagttaac
    50461 aactcattgg cttcaagata gccgatataa ttgatagaag ggattgtttt aagcaccgct
    50521 gaggcatccc gaatactgtc gagacccttt acttcttctt caccaatatt gagcaacgcc
    50581 acgcgaggat tgggaatttc caccacctct tcagccagaa ctgagcccat aatggcaaat
    50641 tgcaccaaca ttgtactgtc acaatcgacg ttggccccta agtcaaggac caccgttttg
    50701 cccttttgct gatgtggtaa taccgtcacc agcgccggac gctcaatccc ctccaggggc
    50761 ttgagtaata attttgccag ccccatcagt gccccggtat taccggcact gacacaggct
    50821 tgcgctcgac cttctttcac cagctccagg gccacgcgca ttgaactccc acgactggcg
    50881 cggatagctt gcgaaggccg ggcatcactg gcgataactg actgcgcagg aataatctgc
    50941 agacgcgaac gttgttcaaa gtcagcttta gcaagtaatg gcgtgatggc gtcgggattg
    51001 ccgactaaaa gaagagtgag ttgcgaatta gaattcagtg cctgcaatgc tgcaggcact
    51061 gtcacggaag ggccaaaatc ccctcccatg acatctaacg ccagggttag acgtgtcaag
    51121 gtatcgtcgc cgcccggttt ggtctttccc cagttgcctg gggaaaatcc tcactaagct
    51181 tcatcacgca gacgcgtgat tacttagcga tgaccttgcg gccgcggtag taaccgtcgg
    51241 cagtgatgtg gtgacgcagg tgtttttcac cagaagtttt gtctacagac aggctggtga
    51301 ctgcggtcag cgcgtcatgg gaacgacgca tgccacgttt ggaacgggtt ggtttattct
    51361 gttgtacggc catggacctt actcctcaat tacggtttat ccccgatcca acggggagca
    51421 ccaattactt acgctttaag ctggctaata cggcaaatgg gtttggcttt tgcacttctt
    51481 caggcagttc accaaagacc atgtccgctt cggacacttc acagtgttca gaatcatgca
    51541 ccggaactac cggcaaggcg aggatgattt catcttcaac cattgcaagc agatcgattt
    51601 caccgaattc gttaacctca atcggttcat acgcttccgg cagtgcttca gcctgttcgt
    51661 ctgaacgcac aggactaaaa caatacgttg tgtagacctg atgagtaaac ggcttcccgc
    51721 aacgctgaca ctcgagcgtt accgtcacct tcgcatcgcc gtttaacact gcgagacgtt
    51781 ggttatcgat agcgaacgac atggagcatt ccacatcact gtccacactg actacggatt
    51841 cggcgacgcg ctcaacctga tcaggggtat agataccctg gtaatcaagg cgtttttgag
    51901 ccgtacgaac cggatcgaga gtcaggggta attttacctt ttgcataggg cgcgcatatt
    51961 aactttgtaa cgtcatagag tcaaagaaaa aggcagcctg tggttgcctt ttgccaataa
    52021 ttcgcacaca ttgcgggtta ctgctttatt ttcgttcagt gctgataacg aacatcaacc
    52081 tgcaaaacga agcgcatagt ttaaaatggc tatcatcaat acgctatatc tgatggaaaa
    52141 aaatatgcct aaacttattt tagcctccac ctcgccatgg cgtcgcgctc ttctggaaaa
    52201 actgcaaatc tcttttgaat gtgcagcacc agaggtcgac gaaaccccac gcagcgacga
    52261 atcaccgcga cagttggtgc ttcgactggc acaagaaaaa gcgcaatctc tggcgtcacg
    52321 ttatccggat catttaatta ttggttcaga ccaggtatgt gttcttgatg gcgaaatcac
    52381 cggtaaaccg ttaacggaag aaaatgcccg tctgcaatta cgcaaagcca gcggcaatat
    52441 cgtcaccttc tatactggac tggcgttgtt taattcggcg aatgggcatc tacaaacaga
    52501 agtggagcct tttgacgtcc atttccgtca cctgagcgag gcggagattg ataattacgt
    52561 gcgtaaggag catcccctgc actgcgcggg tagctttaag agtgaaggat ttggcattac
    52621 gctgtttgag cgtttagagg gacgtgaccc taacacgctg gttggtttac cgctaatcgc
    52681 gctgtgtcag atgttacgtc gggaagggaa aaacccgctg atgggatagt tgtttgcgct
    52741 ggatgcggtt acgtcgcatc cggcaatcgg tgcaggcggc ctgatgcggc gtaaacgcct
    52801 tatcaggcca accgcccatt atcaggctta tatgcttgta ttagcgcgcg ttacgcagct
    52861 tttgcaaaca acgcttcaaa ccttcatcca tcggcgcttc gatacgcatc acctcaccgg
    52921 tccccggatg ggtaaacttc aacgctgcgg cgtgcaggaa cagacgattt aatcccgtgc
    52981 ctgcttcagt gagctgtctg tcaaattcac ggtcaccgta gcgatcgtca aaggcaatcg
    53041 gatgacccgc atactgtgta tgcacacgga tctgatgagt acgtccggtt accgggctac
    53101 aacgcaccag ggtggcaaat gcatagcgtt cttccacttt aaagcgtgtt tccgacggtt
    53161 tgccttcctg actgacacgc acgatacgtt cgccgctttg cagaatattt ttcaataacg
    53221 gcgcttgaac gctcttcaca tgcgactgcc actgaccgcg caccagcgcc aggtaatctt
    53281 tttgcattcc tttttcacgt aattgctcat gcagagaacg caacgccgag cgttttttcg
    53341 ctatcagcaa cacacctgaa gtgtcccggt cgagacgatg aaccagttca aggaaccgcg
    53401 cttccgggcg caacgcccgc aaaccttcga taacgccgaa gcttaaacca ctgccgccat
    53461 gtaccgccgt accggaaggt ttattcagca ccaggatatg atcatcttca tataagatga
    53521 cgtccgccag cgccgccacc ttttgcagat gtggcgaaac cgcctcttct tcccgctcag
    53581 caacgcgaac cggtgggata cgcacctcat cacccgcttc gagtttatat tcaggcttaa
    53641 tacgtttttt gttcacccgc acttcgcctt tacgcaaaat acggtaaatc atactttttg
    53701 gtacgccttt caattgggta cgcaaaaagt tatcgatacg ttgccccgct tcgtcagcgg
    53761 tgatagcaac aatttttacg gatggagtct ctgttttcat ggtgggcgat tctaattagc
    53821 caacaggatt cgcgccactc atttttctat gcttatattt agttttgcac cttattactt
    53881 cactacgtga tcactttatt gatggttatt aaaccaatca ccagcaagaa gtgaaaaaac
    53941 tgtgagtaag cgggtgataa atggtaaaag tcatcttgct ataacaaggc ttgcagtgga
    54001 ataatgaggc cgtttccgtg tccatccttg ttaaaacaag aaattttacg gaataaccca
    54061 ttttgcccga ccgatcatcc acgcagcaat ggcgtaagac gtattgatct ttcaggcagt
    54121 tagcgggctg cgggttgcag tccttaccgg tagatggaaa tatttctgga gagtaatacc
    54181 cagtctgttt ctttgataat tgcgctgttt ttccgcatga aaaacgggca accgacactc
    54241 tgcgcctctt tgagctgacg ataaccgtga ggttggcgac gcgactagac acgaggccat
    54301 cggttcacac ccggaaaggc gttactttgc ccgcagctta gtcgtcaatg taagaataat
    54361 gagtaagtta cgatgaaaag aatgttaatc aacgcaactc agcaggaaga gttgcgcgtt
    54421 gcccttgtag atgggcagcg tctttatgac ctggatatcg aaagtccagg gcacgagcag
    54481 aaaaaggcaa acatctacaa aggtaaaatc acccgcattg aaccgagtct ggaagctgct
    54541 tttgttgatt acggcgctga acgtcacggt ttcctcccac taaaagaaat tgcccgcgaa
    54601 tatttccctg ctaactacag tgctcatggt cgtcccaaca ttaaagatgt gttgcgtgaa
    54661 ggtcaggaag tcattgttca gatcgataaa gaagagcgcg gcaacaaagg cgcggcatta
    54721 accaccttta tcagtctggc gggtagctat ctggttctga tgccaaataa cccgcgcgca
    54781 ggtggcattt ctcgccgtat cgaaggcgat gaccgtaccg aattaaaaga agcactggca
    54841 agccttgaac tgccggaagg catggggctt atcgtgcgca ccgctggcgt aggcaaatct
    54901 gctgaggcgc tgcaatggga tttaagcttc cgcctgaaac actgggaagc catcaaaaaa
    54961 gccgctgaaa gccgcccggc tccgttcctg attcatcagg agagcaacgt aatcgttcgc
    55021 gcattccgcg attacttacg tcaggacatc ggcgaaatcc ttatcgataa cccgaaagtg
    55081 ctcgaactgg cacgtcagca tatcgctgca ttaggtcgcc cggatttcag cagcaaaatc
    55141 aaactgtata ccggcgagat cccgctgttc agccactacc agattgagtc gcagatcgag
    55201 tccgccttcc agcgtgaagt ccgtctgccg tccggtggtt cgattgttat cgacagcacc
    55261 gaagcgttaa cggccatcga catcaactcc gcacgcgcga cccgcggcgg cgatatcgaa
    55321 gaaaccgcgt ttaacaccaa cctcgaagct gccgatgaga ttgctcgtca gctgcgcctg
    55381 cgtgacctcg gcggcctgat tgttatcgac ttcatcgaca tgacgcctgt acgccaccag
    55441 cgtgcggtag aaaaccgtct gcgtgaagcg gtgcgtcagg accgtgcgcg tattcaaatc
    55501 agccatattt ctcgctttgg cctgctggaa atgtcccgcc agcgcctgag cccatcactg
    55561 ggtgaatcca gccatcacgt ctgcccgcgc tgctccggta ccggtaccgt gcgtgacaac
    55621 gaatcgctgt cgctctctat tctgcgtttg atcgaagaag aagcgctgaa agagaacacc
    55681 caggaagttc acgccattgt tcctgtgcca atcgcttctt atctgctgaa tgaaaaacgt
    55741 tctgcggtga atgcgattga aacgcgtcag gacggtgttc gctgcgtgat tgtgccaaac
    55801 gatcagatgg aaaccccgca ctaccacgtg ttgcgcgtgc gcaaagggga agaaacgccg
    55861 accttaagct acatgctgcc gaagctgcat gaagaagcga tggcgctgcc gtctgaagaa
    55921 gagttcgctg aacgtaagcg tcctgaacaa cctgcactgg caacctttgc catgccggat
    55981 gtgccgccag cgccaacccc agctgaacct gccgcgactg tcgtagcccc agcaccgaaa
    56041 gctgcaacgg caacaccagc agctcctgca caacctgggc tgttaagccg cttcttcggc
    56101 gcactgaaag cgctgttcag cggtggtgaa gaagccaaac cgaccgagca accaacaccg
    56161 aaagcagaag cgaaaccgga acgtcaacag gatcgtcgca aacctcgtca gagcaaccgc
    56221 cgtgaccgta atgagcgccg cgacacccgt agtgaacgta ctgaaggcag cgataatcgc
    56281 gaagaaaacc gtcgtaatcg tcgccaggca cagcagcaga ctgccgagac gcgtgagagc
    56341 cgtcagcagg ctgaggtaac ggaaaaagca cgtaccaccg acgagcagca agcgccgcgt
    56401 cgtgaacgta gccgccgccg taacgatgat aaacgtcagg cgcaacaaga agcgaaggcg
    56461 ctgaatgttg aagagcaatc tgttcaggaa accgaacagg aagaacgtgt acgtccggtt
    56521 cagccgcgtc gtaaacagcg tcagctcaat cagaaagtgc gttacgagca aagcgtagcc
    56581 gaagaagcgg tagtcgcacc ggtggttgaa gaaactgccg ctgccgaacc aattgttcag
    56641 gaagctccag ctccacgcac agaactggtg aaagtcccgc tgccagtcgt agcgcaaact
    56701 gcaccagaac agcaagaaga gaacaatgct gataaccgtg acaacggtgg catgccgcgt
    56761 cgttctcgcc gctcgcctcg tcacctgcgc gtgagtggtc agcgtcgtcg tcgctatcgt
    56821 gacgagcgtt atccaaccca gtcgccaatg ccgttgaccg tagcgtgcgc gtctccggaa
    56881 ctggcctctg gcaaagtctg gatccgctat ccaattgtac gtccgcaaga tgtacaagtt
    56941 gaagagcagc gcgaacagga agaagtacaa gtgcagccga tggtgactgg ggtccctgtc
    57001 gccgccgctg tcgaacctgt tgttagcgca ccggttgttg aagaagtggc tgaagttgta
    57061 gaagcccccg ttcagattgc cgaaccgcaa ccggaagtgg ttgaaacgac gcatcctgaa
    57121 gtgattgccg ccgcagtaac tgaacagccg caggtgatta ccgagtctga tgttgccgta
    57181 gcccaggaag ttgcagaaca cgcagaaccg gtggttgaac cgcaggaaga gacggcagat
    57241 attgaagaag ttgccgaaac tgctgaggtt gtggttgctg aacctgaagt tgttgctcaa
    57301 cctgccgcgc cagtcgtcgc tgaagtcgca gcagaagttg aaacggtagc tgcggttgaa
    57361 cctgaggtca ccgttgagca taaccacgct acggtgccga tgacgcgcgc tccggcaccg
    57421 gaatatgttc cggatgcacc gcgtcacagt gactggcagc gccctacttt tgccttcgaa
    57481 ggtaaaggtg ccgcaggtgg tcatacggca acacatcatg cctctgccgc tcctgcgcgt
    57541 ccgcaacctg ttgagtaata attagctcaa gtaatcaagc cctggtaact gccagggctt
    57601 ttttatttca tctttgaatc aacatattaa aattcatcga atggcatcct tgctaaccaa
    57661 caatgcaaaa taggcaaaaa aatgcccgcc ccggcgatcc cagagcaggc agacaaaaca
    57721 tacccagttt catgatatgt tttaaagcga aattatttgt tgagctggaa gagcgacaat
    57781 ccctgcatat cggtaaatgc tttatacgat gcctgcaatg ccgtttgctg catgatgtaa
    57841 gatgaaatag ttgcattcca gtccacatca accagatcgc tcatctgctg cgtttgccct
    57901 aaagcgcgat cgctacctaa tgaatccagc gactccagtt cgttcagctg cgtgcctaat
    57961 tccgcgcgaa cggtcagcac attgttcagc gagtttttca gtccgcggtt ggttttatct
    58021 aacgccgcag cggcggtttc tttatccgct tcgctatccg cgaccggcgt tttcagggct
    58081 gcgatggcgc tatccagcat ggcaaaaaga ttggtttcag aagcgctacc gtctggttcc
    58141 gctaccgcgt tactggtaat actgtcgaaa attttgtcgc ccgtatgtcc tatcaccatc
    58201 gaacgcgaag catcgacctg ttgcttaata ctttctgttc cgccgacata atcaccgtta
    58261 gcctcactaa acggcgcagt ctctgtttta taaccggcaa aaatgtagcg cccattaccg
    58321 tcagtggtgt tcgccagatt cagcaactgg tcacgaagcc cctgaatatc cgtagccagc
    58381 gaggcccggt catcgtcact caaggtgcca ttgctggcgt agacaatttt ttcctgagca
    58441 ttctggatag cagtggtgac ctggctaagt acactctctt ccagtgacac tttttgagtg
    58501 gcgaaagtac gcgccagcgt gtactggctg ttttgcgcct gtgcctggga gagaacaacc
    58561 gcttgtgatg cagcaatggg atcatctgaa gggttaacga ctcgcttacc cgtcgacatc
    58621 tgttcgccgt acttcatcca ttctgcctga gaattggtga taccacgcat gttttgctgg
    58681 tacatcatct gggtactgaa acgcatttta tctcccctta gcgaatgtta atcagcgcat
    58741 caaaaatcgc gtttgccgtc tgcagaacct gcgcattcgc caggtaatac tgctgaaaac
    58801 gttgcagatt tccgtactcc tcatcgagat tgacaccgga aatcgactgc tgctgattgg
    58861 aaagctgcgt caccacatta ccttgcgtgg tgctactggt tttcaacgtc gcggttttat
    58921 taccgatatc actcactaac gaggcataag cgtcgttaaa ggatttcgca ccgcccaccg
    58981 ttttactatt gctttgcaga tccagcaggg cctgaccgtt gcggttatcg ctatcacccg
    59041 catctttttc gctcgccatc gctattttcg cttcgtcggt gattaataca tccatgttga
    59101 cgatggcgtc acttactggt ttcagcgtga agctgtcgtt aacggcaggc gttcctgtaa
    59161 acgtcaactc cagaccatca aatgccactt taccgttggc atccggcgtc accgtaaaag
    59221 tggtattgct ggcaaggcgg gtgacctgcc actgattatt atcgaacgag attttgtaat
    59281 ctgtcgccag taccacggag gcatcagtta ccgtggcacc gatcgcaacg tcacctttgt
    59341 ttttcgtgtt ttgcagaacc gcgggcttac cgatagtaaa gaaatcttca ccagcatcgc
    59401 cgttagcatc aaatccggct ttgtgttggg tgttgaaagc ctcggcaaat gccagcgcca
    59461 gttgtccaag cgtattacgc gtctggtcca gctcctgaga acggaatgtc agaatgccgc
    59521 ccagcgaccc ggtattcagt aatttctccg ggatctcaat attgcctgcc gtcccatcaa
    59581 cataagcgac agtcgtacga gaagggtcag cgctggaagg aactgccgcc agttgccgcg
    59641 ccgtacttcc ctgaaccagt gagtaaccat tggccaccgt gatgttataa gtgccgccat
    59701 cctgaacgct gacttctaca ccaacaatct ggtttaattc gctcaccagt tgatcgcgtt
    59761 gatccagcag attgttaggt gacgcccctg cccccacgcc tgtcaggcgc gagatttgat
    59821 cgttcaggct ggcaatttgt ttagcgtaat tgttgatctg atcaacgctg gcacctatcg
    59881 cgatattgac ctgtttgtcc tggtcgcgca gatattgatc ggtggtttta aactgattca
    59941 ccaacccttc tgatttccca atcagcgcct ggcgcgctgc cgggtcttcc gcgttactca
    60001 ccagcgtttg caggctggtg aagaaatcct gcatctgtgt tgccagcgaa gaggtactgg
    60061 tggagagcat attgtcgatt ttcgacatct gctcatagcg ggcggtcagg ccgctacttt
    60121 gcgtctgcgc agcacgtaac tggttggtaa taaacgcatc atactcacgc tgcacaccag
    60181 aaacgtagac gccattgcca acccagccgc cagcgcccaa cgtgctattg gcctgcgcca
    60241 taatagtggt ttggcgggta tatccggcaa cgttatagct ggagatatta ttacttgccg
    60301 tatttaacgc cgcctgggcc gcgttcagtc cgctcatggc gttattgatc aagctggaca
    60361 tggaggttcc ttataagcct tcaattacag agtattatcg gcagcgaccc gccggacttg
    60421 agttattcag aacagattat caatgttcat gctgtaggtt ttgctcacct tgtcgcttat
    60481 cgatttcatc tgctggatca tgttggtgag tttacgggca tagtgaggat cggtagcata
    60541 ccccgcgtcc tgtagggcct gcgccccctg ctccgcactc gcggctgtcg tcacggcggc
    60601 gtagcgcggg ttacgtgtta acagcccaac gtaatccgac aatgcttcca gatacgagct
    60661 gtagacccga aatttcgctt ttactttctt cgcttcgcca ttttcatatt cagtcgtggt
    60721 gatttcagta actggacctt tccagttgcc agaggctttg acgccaaaca ggttatagct
    60781 cggctcgccg ttttcgcggc ggatttgccg ttgcccccaa ccagactcca gcgccgcctg
    60841 agcgaggatc aaatgatgtg gcacaccgct ttgctggctt gccagttggg cgggcagcga
    60901 gagttgcgcg aggaatgctt tactgtcacc cggcagcgaa tcatcgtagt tacgtggcac
    60961 ggccttttgc accagctgcg aaagcgcctg attttgataa cgcaccacag tttcgagcgg
    61021 gaatttcatc ggtgctgctg gcgtggactc ctctggcaat ggttgttctg gcgtcatctg
    61081 tttaaccatc atctctgcaa gccccagacc tttgcccgcc gtcatctgtt gggcaatctg
    61141 ctggtcatac atactggtat acaggcgagt gtgctcgctg ctgaacaggc catcttttgg
    61201 taaagcgtcg cgcatgcttt tcaacatcat ctgcacgaac atcccttcca cctgacgggc
    61261 caccggacgg atatttgccg ccggatcttc gcccgctttc gcctttagtt cgttgagtga
    61321 ttgcgcatcc caggccgcac ttgccagtag tttgctgtcg ctgatcatca gatgatttcc
    61381 agttttgccc gcagacatcc cgcactttgc attgattgca gtatggacat cagatccatc
    61441 ggcgtagcgc ccagcgcatt gagcgcgcgc accacgttat tgaggctggc gctggaacgt
    61501 acgctttgca gcgaaccgcc gctctggcgt aaatcgatct gcgtttgtgg agtaaccaca
    61561 gtctgtccac caccaaacgg tgtatctggc tggctgacat tggcctgacg attgactgtt
    61621 actgagagat ttccctgcgc taccgcgcag ctgtcgaggg tcacttcgcg attcatcacc
    61681 accgaaccgg tgcgcgagtt aatcactact ttagcgtcct gcggggtgac attaacctgc
    61741 atattctgga tatctgcaag gaaacggacc tgggaactgt tgccacttgg tacgcgcacc
    61801 tgaatagtcc gcgcatctaa cgcggtggcg ctgccatatc cacgcacgcg gttgatggtg
    61861 tcagcgattt gctgcgccat gctgaaatct tcgtcgttaa gttgcaaatt aagggtattc
    61921 ccgacgccaa actggctggg caattcacgt tcaataaccg caccattggt gatccgtcca
    61981 ccgttcagtt ggttcacctg aacactgcta ccgccagcgg aggctcctgc gccgccaacc
    62041 agaatattgc cctgcgccag cgcatacacc tgactgtcaa cgcccttaag cggcgtcatc
    62101 aacaacgtac ctccacgcaa gcttttggca tttcccatgg aagaaaccac cacatcgatg
    62161 gtttgcccct gacgtccaaa cggaggaagt gactctgtca ccattaccgc agcgacgttt
    62221 tttagctgca tattggtgcc cgtcggaacg gtaattccca gctgtgagag catgttatta
    62281 agcgtttgtg tggtaaacgg cgtctgggtt gtctggtcac cggtgccatc cagccccacc
    62341 accagaccat agccaatcag tgagttttgc cttaccccct gaacactggt gagatcgcga
    62401 atacgctcag cctgagccgc cgtcgtaacc agtagaagaa ttaatgcaga gagaaattta
    62461 atcaccacag cctcacttac attggcgaca ggttaaggaa gaaacgctgc aaccagccca
    62521 tattttgcgc ttcgttaatg tagccattgc ctacgtattc aatgcgcgca tccgccacct
    62581 gagtagacgg tacggtattg ctgccgctga tagtgcgtgg attaaccacg ccagagaagc
    62641 gaataaattc ggtaccctga ttaatggcaa tctgtttttc acccaccaca tgcaggttgc
    62701 cgttgaccag tacctggtca accgtcaccg tcaacgtgcc gctaaaggta ttgctggcat
    62761 tggccccgcc ctttccgttg aacgtgttac caccggaggc ttcgacatcg gcacgagcgt
    62821 taccaaacag cccctgcaaa tagcgcggca cagtatcaaa gccaaaatta gttttaccgt
    62881 cacggctggc attcgcagag gagcttttgc tggcgctgac gttctcctgc aacacgatgg
    62941 tcagcgtatc gccaatattg cgtggtcgac gatcttcaaa cagcggttga tagccatagt
    63001 taatcggctg agcagactgg aaaatagaac cgttggcgac gggcgtcgga ccgggaaccg
    63061 gttgtgcact ggtcgccccc tgcaccagcg gcgtggaggg tatccaggcg cagccggtta
    63121 gtgaaagcac caacaagctg gaaatggcat aagtatgcgc agcgtttttt tgcatggcta
    63181 tcatcttcaa aaatcagtaa accggtgaac ctgccaccgg ttaagcctta gagttgcgtc
    63241 agtttttgca gcatctgatc ggtggtggac accgctttac tgttgatttc gtaagcgcgt
    63301 tgcacctgaa tcatattgac cagttcttcc gccacgttga cgttagacgt ttcaacatac
    63361 ccttgataca gcagtcccgc gccgttcagg cccggcgtgc tttcgttcgg tacaccagag
    63421 gattgcgttt cggtgtagag gttttcgcca atgctctcca gcccggtgtc attcataaag
    63481 gtggtgagat tgagctgccc aacctgaacc ggagctgcct ggccttgttg ggttacgctg
    63541 accacgccat cacgaccgat ggtgatactt aacgcattcg ccggaatggt gatcgctggc
    63601 tgcacctgaa aaccaccagc tgcccgttct gatccacctg gaaagagccg tcacgggtat
    63661 aggctgatga accatctggc aacatcacct ggaaaaagcc ctgcccttta atcgcgacat
    63721 ctttgctgtt gttggtctgc gacaggtttc cctggctgtg taagcgttca gtggcgaccg
    63781 ggcgtacccc cgtgccgatt tgtaatccgg agggtaaggt ggtttgttcg gaagactgtg
    63841 cccccggctg gcgaatggtt tgataaagca gatcttcaaa caccgcgcgc tgacgcttaa
    63901 aaccgttagt actgacgttt gccaggttgt tggcaatgac gtccatattg gtttgctggg
    63961 cgtcaaggcc cgttttggcg atccataatg aactgatcat aaaatgtcct gtagtggatt
    64021 agtgacaaag tgcatatgtc ttcttgccgg atgcggcgta aacgccttat ccggcctaca
    64081 agtgctcgca aaatcgataa attgcgattg cccccataga cccgataaac ttgcgcatcg
    64141 ggcaacttat acttgtcatg tatcctttca attaactcat cgacagcagt tggttggcac
    64201 ggcctgcgtt atcatcgacg ctgctgatca ccttcatctg catttcaaaa cgccgcgcgc
    64261 tggcaatcat gtcgctcatt gccgcaacgg cattgacgtt actgccttcc agaacccccg
    64321 acatcacacg caaggttgga tctgcctgca gtaccggccc tcgcgtggcc tgggtttctg
    64381 cgcttaaacg aaaaatgccg tcgtcaccgc gctgcacttc gctgcccgtg gctttcacca
    64441 gtttcagacg ccctactggc gcaactgtat ttgccggatc gcccggattg agcgccgaga
    64501 ttgtgccatc ggcagcaata gtgatttccg ctccttccgg cacagcaatt ggccccgcct
    64561 cgcctatcac ctgatgcccc tgaattgtca gttgcccggt gggatcaacc tgaatgctgc
    64621 cattacgcgt atacccttcg ctgccgtcag cggtctgcac ggccagccag ccatcctgct
    64681 gcaacgcgac gtccagcggg cgcgaggtgt aatccatttt gccgggcgtc atatctgcgc
    64741 ccggcgttga cgccgtgacc aacgtgcgcg tgggcagaga aagcccttcc actggcaccg
    64801 cgcgtaaagc attcaactgc gcgcgaaaac cgggcgttga ggcattggcc agattactgg
    64861 cggttaccgc ctgttgattc agtgtctggc tggctgctcc catcgcggta taaattgcgt
    64921 gatccattga gctatcccgt cagcgattag cgtaagttaa ccagcgtgtt gaggatctgg
    64981 tcctgagttt tgatggtctg ggcgttagac tgatagttac gctgggcaac gatcatattg
    65041 accagttctt tactgagatc gacattggac gcttccaacg caccgttggt cagggtgcca
    65101 aagttgcccg tcccggctgt ccccaacagc gccacgccag aagattgcgt cgcagaccag
    65161 acgttgtcgc cttcggatgc cagaccttcg ttgttggcaa agttcgccag tacaatctgc
    65221 cccagcagtt gtgtttgttc gttggaatag ttgccgacaa ccgtaccgtc atcattgatt
    65281 tgataactca ccagatcgcc cggtttgtag ccattctggg tggttgccac aatattgtta
    65341 gcgccggtat tttgctgcat ggagttgagg aagctcaggc taaacgtggc gggatctgcg
    65401 ccgttaattg cgccagtggt aatattctct gttggatttg aggtcagaac gccattggca
    65461 ttaaacacca gcttcattgc aggctcggct gtacctgttg gatcactgct atcctgggtg
    65521 tagacctgcc agttattatc cccggtcttc acaaagtaga cgctcatgtc atgagcatta
    65581 ccctgactgt cgaaaacagt caccgaacct tttttgttat agctatccgc attgctggcg
    65641 ctgaatggcg taacagtagg aagcggatca ctggaattca ggttgatctg catcgacgcc
    65701 gtggtggtag ttttcgctgc catcagggta ttcgggatcg aaatattggt cggattcgcc
    65761 ccttgctgaa tagtcggcgg cgtaccggtt gccgggtaac ccgtcagctg taaaccttgc
    65821 atattcacca ggttacggtt ttcatccagc ttaaattgtc cgttacggct gtagaacacc
    65881 gaaccgttgc tgtctaccag acggaaaaaa ccgttctggc tgatagcaac gtccagacct
    65941 cgcccggtgt tggtggtcgt gccatcggta aagtcctgag tgataccggc aacttttacc
    66001 cccagtccca ctttcgaacc ggcaaacata tcggcaaaag aggccgtgcc tgatttaaag
    66061 ccgtaggtgg cggagttggc gatattgttg ccaataacat cgaggttggt ggcggcagcg
    66121 tttaatccgc taaccgcttg agaaaaggcc atgactgact cctgaaatgt gaaggcttaa
    66181 attatctgcc gtacttcgtc gagggtggtg gtgccgtaag tgccgagatc cagcgtatta
    66241 ccgctgttgc cgcggagcac accctgcacc agagcaaact gcagcggctg ggcaaccagt
    66301 tgtgtaccac cgttactggc gctaatcgct acattgtaag aaccgttcgg cgcagtgctg
    66361 ccatcagtca acgtaccgtc ccaggtgaaa ctgtggactc cggcggtcag ttcaccaata
    66421 tcaatggtgc gcacaaccgc gccattttta tcggtgatgg tggccgtaac tttgtctgcc
    66481 gcctgttgca gctcaacacc aaacggcgtg gtcgtggtca cagccccttc ttcactgccg
    66541 gttcccgcaa gaacagtggt gccggggatc atcacgccgt gaccgatcag gttactggcc
    66601 tgtaacgact ggctgttatc aatctgtccg gaaatagatc cgagcgtggt attgagtttt
    66661 tcaatcccgc tgaccgtgct gatttgtgcc aattgcgacg tcagctcgtt gttttccatt
    66721 ggattggtcg ggtcctggtt tttcagctgc gccaccaaca aagtcagaaa actgctttgt
    66781 aagtctgcgg cgttgctgcc cgtgagcgaa ctactgctgg tggtactgac gccggtattt
    66841 gtcggatcgg tggtggttac cgcaatggac atagctttct cctttattga ccgagcgtaa
    66901 gggttttcag catcatgctt ttcaccgtgt tgagcacttc aacattggcc tgatagctgc
    66961 gtgacgccga catggtgtta accatctccc cgacgacatc aacgttcggc atttttacgt
    67021 agccctttgc atccgccagc ggattacccg gttcataaac cagtttgtcc ggggcctgac
    67081 tttctataac atcggcaacc tttacgccgc ctgtcgcagc acctggtgca gcgttaacct
    67141 ggaataccac ctgttttgcc cgatatggct gtccatcggg accggtcacg ctatcagcat
    67201 tcgccagatt actggccgcc acgttcaagc gctgggactg ggcagttaac gccgacccgg
    67261 cgatatcaaa aatattcagc agtgccatcc gttaatttcc gctctgtaaa acgttcatca
    67321 tgcctttgat ttgcccgctc aacgcgctaa ggctcatctg gtattgcagg ctgttatcgg
    67381 caaactgggt gcgttcgcga tccatatcga cggtattacc gtcaagcgaa ggctggtccg
    67441 gaatacggta ttgcagttct gcggtaggag gcgtcagcgc ctgcgccgga atgtgttgcg
    67501 ttgaggtcat cgtcagtgca accacactgg ttgcatcccg tccacgttgc atgacttttt
    67561 taagttcact ggcaaaatcg atatcgcgcg cctgataacc aggggtatcg gcattggcga
    67621 tgtttgctgc cagcacttcc tgacgctggg cgcgcagatt gagcgcctct tgttgaaaac
    67681 gtaaggcggc gtcgagctta tcgagcatat ctcctccgca ggtatcaaaa ttctgccatc
    67741 agcttaaatg cctttacgcg cgcgttatcg gcggaataaa cgcaaaatgg gtcgctattt
    67801 atgccgttga tgaccattgc ggacaggtac aattcacgtt gtagaaatgg ctgggggcga
    67861 aaatgctggc aataaaacgt agcgtggcga tcatctcgat actattcact ccgttaagtg
    67921 cggcgagtaa tctcacatcg caattgcaaa ccttttttag cgcccagctc gcgggggtaa
    67981 gtgatgaggt tcgtgtttct attcgtacag cgcccaatct actaccgccg tgcgagcagc
    68041 cattgctttc gatgagcaat aattcccgcc tgtggggcaa tgtgaatgtg ttggcacgct
    68101 gcggtaacga caaacgatat ttacaggtta atgtacaggc cacaggaaat tatgtggttg
    68161 ccgcgatgcc cattgtgcgg ggaggaaagc tggaagctgg caatgtcaaa ctaaaacgcg
    68221 gacggcagga taccctgcca ccgcgtacgg tgctggatat caatcaactt gttgatgccg
    68281 ttagcctgcg cgatctatca cccgatcaac ctatccagtt aacccagttt cgccaggcat
    68341 ggcgggtaaa agcgggacaa cgcgtcaacg tgatcgccag cggtgacggg tttagcgcca
    68401 acgcagaagg tcaggcgctg aacaatgcag ccgtcacaca gaatgcgcgg gtgcgcatgg
    68461 tatcgggact ggtggtcagc ggcgttgttg atgcagatgg gaatattctt ataaaccagt
    68521 aagctgttaa agattacccg tcccttgccg ataaataagc aacacatgat aaaagcgccc
    68581 tcaatgagga ataaaccatg agtattgatc gcacttcgcc tctgaagccc gtaagcaccg
    68641 ttcaaccacg cgaaaccact gacgcgtcgg taacgaacac ccgggcggca aaaacaacag
    68701 cctccaccag caccagtgtg acgttaagcg acgcgcaagc aaaactgatg caacccggca
    68761 gcagtgatat caatcttgaa cgtgtcgaag cgttaaaact ggcgattcgt aacggtgaac
    68821 taaaaatgga caccggcaaa attgccgatg cgctgctcaa cgaagcgcag caagacttgc
    68881 agagtaactg atcgtatgac acgtcttgca gagatcctcg accagatgtc cgctgtgctt
    68941 aacgatctca aaacggtaat ggatcaagaa cagcaacatc tctctatggg gcagatcaac
    69001 ggcagccagt tgcaatggat tacagaacaa aaaagctcac tgctggcgac gctggattac
    69061 ctcgaacagt tacgcaggaa agaacccaat acagcaaata gcgttgatat tagtcaacgc
    69121 tggcaggaaa ttactgtgaa aacgcagcaa ctacgccaaa tgaatcaaca taacggctgg
    69181 ttactggaag gacagattga gcgcaatcaa caggcgctgg aaatgttgaa accgcatcag
    69241 gaaccgacgc tatatggggc gaacggtcag acctcaacaa cccatcgcgg cggtaaaaag
    69301 atttcgatct gaatattatc tccggcctgc actgcaggcc ggaatgcatt gttacaccgt
    69361 ccggcgggca aattctttaa ctttgaagcc cagtaccgcc agtgcagcga agtacgcggc
    69421 aatccccgcc agcacgaccg ccattaaacg cagtaagcgc cagggcatgg tacccaatga
    69481 ccactccggc atgatatgta acatacctaa aagcacgcca gacatcacca gtaccgccac
    69541 caccagacgc aacagaaacg ccatccagcc gggttgcgga gtaaagattt tctgcttacg
    69601 caactgccag taaagcagcg aagcattcag acacgccgcc agaccaatag aaagtgacag
    69661 cccagcatgt ttcaacggac caataaacgc caggttcatc aattgcgtta aaatcagcgt
    69721 aacgatggca attttcactg gcgttttaat gtcctggcgg gaataaaagc caggagccaa
    69781 cactttcact acaatcaggc caatcaaacc caccgagtag gcaattaacg cccgctgggt
    69841 catcagcgca tcaaacgcgg taaatttacc gtactggaac agcgaaacgg ttaacggacc
    69901 cgaaagaatg cccaacgcaa ccgcactcgg cagcgccaac aggaaacaaa gacgcaaccc
    69961 ccagtccatc aaacggttgt attcatcatg attgccactg gcaaaacttt tcgacagcga
    70021 cggcagcaaa atggtgccaa gcgccacgcc cagcacaccg gagggaaact ccattaagcg
    70081 gtcggcgtaa tacatccaag acaccgaacc ggaagcaaga aacgaggcaa aaatggtgtt
    70141 gatgattaag gagatctggc tcacagagac gccaaggatc gccggtccca tctgtttcac
    70201 cacgcgcatt gctccggcat cgtggaagtt aatgcgcggc aggaccagca tgccgatctt
    70261 cttcaggtgc ggtagctgat acaccagctg caggatgccg ccgaccgtaa cagcccacgc
    70321 cagcgccagc accggtgggt taaagtacgg tgcggcaaac agcgcgaaac caatcatgct
    70381 gatgttaagc agtgttggcg caaacgccgg aatcgagaag cggttccacg tattcagaat
    70441 cgctcccacc agcgacgcca gggagatcag caagatatag ggaaaggtaa tctttagtag
    70501 ctggctggtc agggcaaatt tgtcagctgt gtcagcgaag cctggcgcgg tcaccatgat
    70561 cacccacggt gcggcgagca tgccagcgac cgtcacaacc gccagcgcaa gtgtcagcag
    70621 gccagaaaca taagagacaa agacccgcgt ggcgtcttca ccctgcttac ttttatattc
    70681 cgccagaatc ggtacaaatg cctgggaaaa tgccccttcg gcaaagatac ggcgtaacaa
    70741 gttaggaagt ttaaaagcga cgaaaaaggc gtcggttgcc atccctgcgc caaagattct
    70801 ggcgacaatt gcgtctcgtg cgaagccaag cacacgcgaa aacatggtca tcgagctgac
    70861 ggcggccagc gattttaata aattcatcgg tgttctaatc cagacccacg ggcaaaacgc
    70921 ctgcaaggca ggcgctgaaa gaggcgaata gtctacctgg attatggtga attgctaccg
    70981 ccagatgtta cagggtttat tcactcatcg catcgcgcca gatcttgtca acgatacgtt
    71041 gcgccagcac tgcctgttcg ccggcggttt gcagaactgt ctgattttgc acacattcaa
    71101 tgaagtgccg cgcacagccg acaaatccac gttgctcaag agtgctctgc caaccaggaa
    71161 tcggtttatg cactacgccc tgcccgcgct cctcacgcca ttcgcgcata tccgtaatgt
    71221 cgatgagcgc atcgtcagtc acggcctgca cggtttcacg ctgacttccg gcacggcgat
    71281 gcatacaggt ggtgatctgc aaaggatcgg ccgaaaaatg gtgctcggca aacagcattt
    71341 cgccagcgtc gttagtcagt agcgtaccgc catccagaga ggctttgccg cccgacaacc
    71401 acagcgcggt atccaccaca tgcagataat catccagcaa cgtgaaataa agatcgtgtg
    71461 gcccgacact attgctacga tgtttatcca ttcttagcga agctgcggta gcgagttgcg
    71521 tttttaactc accgtagagt ggtgcgaaac gacggttaaa accgaccatc aacgtcagtt
    71581 ttttccgcgc cgccagttcc accagccgtt cagcatcgcg cagattttct gccagcggtt
    71641 tatcgacaca gacatgtaca cccgcattga gtaacgtact gaccacgtca aagtggctgg
    71701 cggtgctgga atgcacaaaa accgcatcgc aactggcggc aaggctggat aacgaatcgg
    71761 cataaggaat gcgccagctt tcacaaattg gcagggcttt cgcgcgcgta ggcgaccagg
    71821 ctccttgtaa catccagtca gacgctgccg ccagcaccgg taaccacgct ttttgcgcaa
    71881 tgccgcctaa tcccacaacg ccgatacgta attttttcac tttaatctcc cagatgggcc
    71941 agcaacgaat caagacgctg tttcagttct gccacttcga tttccagggc ttcgacgcgg
    72001 gcctgtaaat caccatcaac cgcgttcgac atatccgtta ccgccggctg atcctctacc
    72061 tcaccactga aaagatgcat gtagcggctt tcgcgtttac ccggttcgcg ggccagacgc
    72121 accacaaaag gaccatcttc gcgatttgcc agttgttcca gcgtcgactc cacttccgcc
    72181 atatcgctga attcatacat tcgcgcggcg cggctgcgca gttcacccgg cgtctgggca
    72241 ccacgcaata acaacgtggt gattaacgcc acttccgctg cgctcagttt cagatcgcca
    72301 aattctgaat tacaaaaacg ttgttcatat ttggtgaccc gattaccaaa gccactcacc
    72361 gtgcgcagat aatggcgttt gaccagatta tccagttgct cctgcacttc agattcactc
    72421 agattcatga ccggttcacg gttcgttttc tgattacagg ccgtgactac accattgact
    72481 gagagcgggt attgctccgg cgtggtcacc tgcttttcca gcaagcaacc aatcacgcga
    72541 gcttcaaggg cagtaagttg atatttcatg agattctcct tagcggccgg gcgtccagtc
    72601 tggggtagtt aatgccgtca gtacgtgatc gcgccattgt ccatcaatca acagatagtc
    72661 tttcgcatag ccttcttttt caaaacccag tcgcgccagt aaatcaccgc tgcgtttatt
    72721 gtgcggcata taattagcca taatgcgatg aatatgttgg gtgcgctgca tataacgaat
    72781 ggctgcggtc agggcttcaa acatgagtcc tttgccctgc catttttgcc caatcgaata
    72841 accgagatag caggcatgaa aagagccacg aacaacattg gaaaaattgg caacgccaat
    72901 aatctctttt tcgtccgggt cgaataagcc aaagtagaaa gctgaacctt gtttatgaaa
    72961 ttcgttaatc atccccagcc tggcctgcca gcctgatgga taacagtggc tttcgtcgcg
    73021 cactggctcc cagggcttga ggaaatggcg attctctgcg taataatccg caagacgcca
    73081 ggcatcacga tcatgcacca gacgcacgac cagtcggtct gtggttaagc gcacttttgg
    73141 cacgttactg cgatagccaa acattctata cctactcctt cccgtaacgt ctttaatacg
    73201 ctgtaagtat tactatacct gcgcaaccag accctgtgaa aagtccaaaa tgccattttc
    73261 tgcattattc acatatttga taaaaactct ctatcaaagc gacgttttga taaaaaaata
    73321 ttgtccccga agtgatggga aaagtcttag cggcaatgca gaatagaaag tgctcatctt
    73381 ttatttcccg ggaggggaaa tgtcccgcgt atcgcaggcg aggaacctgg gtaaatattt
    73441 cctgctcatc gataatatgc tggtcgtgct ggggttcttt gttgtcttcc cgctgatctc
    73501 tatccgcttc gttgatcaaa tgggctgggc cgccgtcatg gtcggtattg ctctcggtct
    73561 acgccagttt attcagcaag gtctgggtat tttcggcggc gcaattgccg accgctttgg
    73621 tgccaaaccg atgattgtta ccggtatgct gatgcgcgcc gccggattcg ccacaatggg
    73681 tatcgcccac gaaccgtggc tattgtggtt ttcatgcctg ctctcgggac tcggcggcac
    73741 gttgtttgat ccgccgcgtt cggcgctggt ggtgaaacta atccgtccac aacaacgttg
    73801 tcgttttttc tcgctgttga tgatgcagga cagtgccggt gcggtcattg gcgcattgtt
    73861 ggggagctgg ctgttgcaat acgactttcg cctggtctgc gccacagggg ccgttttgtt
    73921 tgtgctgtgt gcggcgttca atgcgtggtt gttaccggca tggaaactct ccaccgtacg
    73981 cacgcccgtt cgcgaaggca tgacccgcgt gatgcgtgac aagcgttttg tcacctatgt
    74041 cctgacgctg gcgggttact acatgctggc tgtacaagtg atgctgatgc tgccaattat
    74101 ggtcaacgac gtggctggcg cgccctctgc cgttaaatgg atgtatgcca ttgaagcgtg
    74161 tctgtcgtta acgttgctct accctatcgc ccgctggagt gaaaagcatt ttcgtctgga
    74221 acaccggttg atggctgggc tgttgataat gtcattaagc atgatgccgg tgggcatggt
    74281 cagcggtctg caacaacttt tcaccctgat ttgtctgttt tatatcgggt cgatcattgc
    74341 cgagcctgcg cgtgaaacct taagtgcttt gctggcggac gcaagagctc gcggcagcta
    74401 tatggggttt agccgtctgg gtctggcgat tggcggcgct attggttata tcggtggtgg
    74461 ctggctgttt gacctgggca aatcggcgca ccagccagag cttccgtgga tgatgctggg
    74521 aattattggc atcttcactt tccttgcgct gggttggcag tttagtcaga aacgcgcaac
    74581 gcgtcgtttg ctggaacgcg acgcctgatt tgatactccc ccctgactcc tccatactga
    74641 aagtgaactg acagccaaat ggaggagtca cgtgaagcta tacatttacg atcactgccc
    74701 ttactgcctc aaagcccgca tgattttcgg cctgaaaaat atccccgtcg aattacatgt
    74761 tctgctcaac gacgacgcag aaacacccac ccggatggtc ggtcaaaaac aggttcccat
    74821 tctgcaaaaa gatgacagcc gctatatgcc agaaagcatg gatatcgttc actatgtcga
    74881 taaactcgac ggcaaaccgt tactgaccgg caaacgttcc cctgccattg aagagtggct
    74941 gcgcaaggtc aatggctacg ccaacaaact gctgttgccg cgttttgcca aatcggcatt
    75001 tgatgagttt tctactcccg ccgcgcgcaa atatttcgtc gacaagaaag aggccagcgc
    75061 gggtaatttt gccgacctgc tggcccactc tgacggtctg attaagaata tcagcgatga
    75121 tttgcgtgcg ctggacaaac tgatcgtcaa accgaacgcc gtgaatggcg aactttcgga
    75181 agatgatatt cagctatttc cgctactgcg taatctgacg ctggtagccg gaatcaactg
    75241 gccaagccgc gttgctgatt accgcgataa tatggcgaaa cagacacaaa tcaatttgtt
    75301 atcatcaatg gcgatttaat cctgacccgc cgcggcagcc tgtattcgcg ggttgccgcc
    75361 gttttcagtt ttttctgctg gcgagtttat cgctggtgac acatgctgcg ttgtgtcagg
    75421 atattgacgc agatttcatg tcgaggacta ccatgaacaa gtttttattt gccgctgcat
    75481 tgatcgtcag tggcctgctc gttggctgta atcaactcac ccaatacacc atcaccgaac
    75541 aagaaattaa ccagtcgctt gcgaaacata ataatttctc aaaagatatc ggtttacccg
    75601 gcgtggctga cgcccatatt gttctgacaa acttgatcag ccaaattggt cgcgaagagc
    75661 cgaataaggt taccctaacc ggagacgcca atctggacat gaactccctg ttcggtagtc
    75721 agaaagcgac catgaaactg aagctgaaag cgctgcccgt gtttgataaa gaaaaaggtg
    75781 cgatcttcct gaaagagatg gaagtggtcg atgcgacggt acaaccggaa aaaatgcaaa
    75841 cggtgatgca aacgttgctt ccctatttga accaggcatt acgcaattac tttaaccagc
    75901 aacctgctta cgtcctgcgc gaagatggca gtcagggcga agcaatggcg aaaaaactgg
    75961 cgaaaggcat tgaagtgaag ccaggcgaaa ttgtcattcc gtttacggat taatgacgag
    76021 ggcgcattcg cgccctttat ttttcgtgca aaggaaaacg tttccgctta tcctttgtgt
    76081 ccggcaaaaa cattccttca gccggagcat agagattaat gactgcacca tcccaggtat
    76141 taaagatccg ccgcccagac gactggcacc ttcacctccg cgatggcgac atgttaaaaa
    76201 ctgtcgtgcc gtataccagc gaaatttatg gacgggctat cgtaatgccc aatctggctc
    76261 cgcccgtgac cacagttgag gctgccgtgg cgtatcgcca acgtattctt gacgccgtac
    76321 ctgccgggca cgatttcacc ccattgatga cctgttattt aacagattcg ctggatccta
    76381 atgagctgga gcgcggattt aatgaaggcg tgttcaccgc tgcaaaactt tacccggcaa
    76441 acgcaaccac taactccagc cacggcgtga cgtcaattga cgcaatcatg ccggtacttg
    76501 agcgcatgga aaaaatcggt atgccgctac tggtgcatgg tgaagtgaca catgcagata
    76561 tcgacatttt tgatcgtgaa gcgcgcttta tagaaagcgt gatggaacct ctgcgccagc
    76621 gcctgactgc gctgaaagtc gtttttgagc acatcaccac caaagatgct gccgactatg
    76681 tccgtgacgg aaatgaacgg ctggctgcca ccatcactcc gcagcatctg atgtttaacc
    76741 gcaaccatat gctggttggt ggcgtgcgtc cgcacctgta ttgtttaccc atcctcaaac
    76801 gcaatattca ccaacaggca ttgcgtgaac tggtcgccag cggttttaat cgagtattcc
    76861 tcggtacgga ttctgcgcca catgcacgtc atcgcaaaga gagcagttgc ggctgcgcgg
    76921 gctgcttcaa cgccccaacc gcgctgggca gttacgctac cgtctttgaa gagatgaatg
    76981 ctctgcagca ctttgaagca ttctgttctg taaacggccc gcagttctat gggttgccgg
    77041 tcaacgacac attcatcgaa ctggtacgtg aagagcaaca ggttgctgaa agcatcgcac
    77101 tgactgatga caccctggtg ccattcctcg ccggggaaac ggtacgctgg tccgttaaac
    77161 aataaaaatc gtagcttcct gttgtcatta ggttatttaa cctgtataaa taaccagtat
    77221 attcaacagg gggctattat gcgaattgaa gtcaccatag cgaaaacttc tccattgcca
    77281 gctggggcta ttgacgccct ggctggcgaa ctttcccgcc gtattcagta tgcgtttcct
    77341 gataatgaag gccacgtatc ggtacgttat gccgcagcga ataatttatc ggttattggc
    77401 gcaacaaaag aagataaaca gcgcattagc gaaattctcc aggaaacgtg ggaaagcgcc
    77461 gatgactggt ttgtcagcga ataatatgca gtgatttttg tttttgccgg gtcgccccgg
    77521 cttttttatt ttacttacga attttgagta caaaatttct acacttcctc aaaataattg
    77581 cacaatatgc taaaaaaata tgaatgacat gctgttttat tgttcatata aatcttaaaa
    77641 gcagaaattt atctgttccc ccctttaaaa attcggtgaa tacccttact tattggtata
    77701 ctgaagttgc ttcagataaa aacgcattga acctcgaata acgttgtcta gtaacacgaa
    77761 ttagggggcc atgatggaaa aaaataatga agtcattcag actcatccgc tcgtagggtg
    77821 ggacatcagc accgttgata gctatgatgc gctgatgttg cgtttgcact accagacccc
    77881 aaataagtcc gagcaggaag ggactgaagt tggtcaaacg ctctggttaa ccactgatgt
    77941 tgccagacaa tttatttcga tattagaagc aggaatcgcc aaaattgaat ccggtgattt
    78001 ccaggtaaac gagtatcggc gtcattaaca tgacatgacc atccacccaa aaggcacctc
    78061 accggtgcct ttaccatttc tgggttgtat atctgcattc agttaattac tctgcttaaa
    78121 ccatgagagc agagagaaaa aaatgaaata cgatctcatc attattggca gcggttccgt
    78181 aggcgctgcc gccgggtatt atgcaacccg cgccggttta aacgtgctaa tgaccgacgc
    78241 ccatatgcca ccgcatcaac acggcagcca ccacggcgat acgcgattaa ttcgccatgc
    78301 ttatggtgaa ggcgaaaagt atgtcccgct ggtcctccgc gcgcaaacgc tgtgggatga
    78361 actttcccgc cacaacgaag atgatcccat ttttgtacgc tctggtgtca ttaacctcgg
    78421 cccggctgac tccgcatttc tcgccaacgt cgcccacagc gccgaacagt ggcaactcaa
    78481 cgttgaaaag ctcgatgcgc aagggattat ggcccgctgg ccagaaatac gcgtcccgga
    78541 caactacatc ggcttatttg agactgattc cggttttttg cgcagcgaac tggcgattaa
    78601 aacctggatc caactggcga aggaagcggg ctgtgcgcaa ctgttcaact gcccggtcac
    78661 cgcaattcgt catgacgatg atggcgtaac tattgaaacg gttgacggtg agtatcaggc
    78721 gaaaaaagcg attgtctgcg cgggaacatg ggtaaaagac ctgctcccgg agctgcctgt
    78781 ccagcccgta cgtaaagtat ttgcctggta tcaggccgat ggccgctata gcgtgaagaa
    78841 taaattcccg gcgtttaccg gtgaactgcc caatggcgat caatattatg gttttccggc
    78901 agaaaacgac gcgttgaaga ttggcaaaca taacggaggc caggttatcc attcagcgga
    78961 tgaacgtgtt ccgtttgcgg aagtggtcag cgatggttcg gaagccttcc cgttcttgcg
    79021 caatgtattg ccgggtatcg gttgctgcct gtacggcgct gcctgcacct atgataattc
    79081 gcctgacgaa gattttatta tcgataccct acccggccac gataatacac tgctcattac
    79141 cggcctgagt gggcacggtt ttaaatttgc gtcagtttta ggggaaatag ctgccgattt
    79201 tgcgcaagac aaaaaaagcg attttgattt gacgccattc aggctttccc gcttccaata
    79261 atcatcatta cggcctccag aatgaggccg tttttacagg agtatttaat gcgtcggctg
    79321 ttgcactatc tca
//
LOCUS       DAJVRF010000003        67338 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJVRF010000003 DAJVRF010000000
VERSION     DAJVRF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67338
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.003"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..1142)
                     /gene="bglX"
                     /locus_tag="OQN13_000167"
     CDS             complement(<1..1142)
                     /gene="bglX"
                     /locus_tag="OQN13_000167"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCS1613644.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESR"
     gene            1338..3053
                     /gene="dld"
                     /locus_tag="OQN13_000168"
     CDS             1338..3053
                     /gene="dld"
                     /locus_tag="OQN13_000168"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS1613645.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(3092..4024)
                     /gene="pbpG"
                     /locus_tag="OQN13_000169"
     CDS             complement(3092..4024)
                     /gene="pbpG"
                     /locus_tag="OQN13_000169"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCS1613646.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMLVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(4198..4785)
                     /locus_tag="OQN13_000170"
     CDS             complement(4198..4785)
                     /locus_tag="OQN13_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCS1613647.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            4955..5533
                     /locus_tag="OQN13_000171"
     CDS             4955..5533
                     /locus_tag="OQN13_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1613648.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(5663..6424)
                     /locus_tag="OQN13_000172"
     CDS             complement(5663..6424)
                     /locus_tag="OQN13_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1613649.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(6477..7913)
                     /gene="mdtQ"
                     /locus_tag="OQN13_000173"
     CDS             complement(6477..7913)
                     /gene="mdtQ"
                     /locus_tag="OQN13_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCS1613650.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIVGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            8137..8220
                     /gene="yohP"
                     /locus_tag="OQN13_000174"
     CDS             8137..8220
                     /gene="yohP"
                     /locus_tag="OQN13_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCS1613651.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(8593..9540)
                     /gene="dusC"
                     /locus_tag="OQN13_000175"
     CDS             complement(8593..9540)
                     /gene="dusC"
                     /locus_tag="OQN13_000175"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCS1613652.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            9779..10177
                     /locus_tag="OQN13_000176"
     CDS             9779..10177
                     /locus_tag="OQN13_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCS1613653.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            10174..10869
                     /locus_tag="OQN13_000177"
     CDS             10174..10869
                     /locus_tag="OQN13_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCS1613654.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            10999..11883
                     /gene="cdd"
                     /locus_tag="OQN13_000178"
     CDS             10999..11883
                     /gene="cdd"
                     /locus_tag="OQN13_000178"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCS1613655.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            12033..12752
                     /gene="sanA"
                     /locus_tag="OQN13_000179"
     CDS             12033..12752
                     /gene="sanA"
                     /locus_tag="OQN13_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCS1613656.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            12755..12994
                     /locus_tag="OQN13_000180"
     CDS             12755..12994
                     /locus_tag="OQN13_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCS1613657.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            13188..14426
                     /gene="preT"
                     /locus_tag="OQN13_000181"
                     /pseudo
     CDS             13188..14426
                     /gene="preT"
                     /locus_tag="OQN13_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            14420..15655
                     /gene="preA"
                     /locus_tag="OQN13_000182"
     CDS             14420..15655
                     /gene="preA"
                     /locus_tag="OQN13_000182"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCS1613658.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(15782..16792)
                     /gene="mglC"
                     /locus_tag="OQN13_000183"
     CDS             complement(15782..16792)
                     /gene="mglC"
                     /locus_tag="OQN13_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCS1613659.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(16808..18328)
                     /gene="mglA"
                     /locus_tag="OQN13_000184"
     CDS             complement(16808..18328)
                     /gene="mglA"
                     /locus_tag="OQN13_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCS1613660.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(18389..19387)
                     /gene="mglB"
                     /locus_tag="OQN13_000185"
     CDS             complement(18389..19387)
                     /gene="mglB"
                     /locus_tag="OQN13_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCS1613661.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(19667..20707)
                     /gene="galS"
                     /locus_tag="OQN13_000186"
                     /pseudo
     CDS             complement(19667..20707)
                     /gene="galS"
                     /locus_tag="OQN13_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            complement(20849..22006)
                     /locus_tag="OQN13_000187"
     CDS             complement(20849..22006)
                     /locus_tag="OQN13_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCS1613662.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(22023..22691)
                     /gene="folE"
                     /locus_tag="OQN13_000188"
     CDS             complement(22023..22691)
                     /gene="folE"
                     /locus_tag="OQN13_000188"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCS1613663.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            22949..23785
                     /gene="yeiG"
                     /locus_tag="OQN13_000189"
     CDS             22949..23785
                     /gene="yeiG"
                     /locus_tag="OQN13_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCS1613664.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(23817..25808)
                     /gene="cirA"
                     /locus_tag="OQN13_000190"
     CDS             complement(23817..25808)
                     /gene="cirA"
                     /locus_tag="OQN13_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCS1613665.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(26100..27569)
                     /gene="lysP"
                     /locus_tag="OQN13_000191"
     CDS             complement(26100..27569)
                     /gene="lysP"
                     /locus_tag="OQN13_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCS1613666.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(27774..28655)
                     /locus_tag="OQN13_000192"
     CDS             complement(27774..28655)
                     /locus_tag="OQN13_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1613667.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            28754..29803
                     /locus_tag="OQN13_000193"
     CDS             28754..29803
                     /locus_tag="OQN13_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCS1613668.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            29877..30734
                     /gene="nfo"
                     /locus_tag="OQN13_000194"
     CDS             29877..30734
                     /gene="nfo"
                     /locus_tag="OQN13_000194"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCS1613669.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAVEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKFRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            30738..31826
                     /locus_tag="OQN13_000195"
     CDS             30738..31826
                     /locus_tag="OQN13_000195"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1613670.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(31882..33132)
                     /locus_tag="OQN13_000196"
     CDS             complement(31882..33132)
                     /locus_tag="OQN13_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCS1613671.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(33232..34173)
                     /gene="rihB"
                     /locus_tag="OQN13_000197"
     CDS             complement(33232..34173)
                     /gene="rihB"
                     /locus_tag="OQN13_000197"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCS1613672.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            34303..35001
                     /gene="yeiL"
                     /locus_tag="OQN13_000198"
     CDS             34303..35001
                     /gene="yeiL"
                     /locus_tag="OQN13_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HCS1613673.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(35068..36318)
                     /locus_tag="OQN13_000199"
     CDS             complement(35068..36318)
                     /locus_tag="OQN13_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCS1613674.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(36412..37350)
                     /gene="psuG"
                     /locus_tag="OQN13_000200"
     CDS             complement(36412..37350)
                     /gene="psuG"
                     /locus_tag="OQN13_000200"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCS1613675.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(37338..38279)
                     /locus_tag="OQN13_000201"
     CDS             complement(37338..38279)
                     /locus_tag="OQN13_000201"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCS1613676.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEVLVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(38737..40428)
                     /gene="fruA"
                     /locus_tag="OQN13_000202"
     CDS             complement(38737..40428)
                     /gene="fruA"
                     /locus_tag="OQN13_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCS1613677.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(40445..41383)
                     /gene="fruK"
                     /locus_tag="OQN13_000203"
     CDS             complement(40445..41383)
                     /gene="fruK"
                     /locus_tag="OQN13_000203"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCS1613678.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(41383..42513)
                     /gene="fruB"
                     /locus_tag="OQN13_000204"
     CDS             complement(41383..42513)
                     /gene="fruB"
                     /locus_tag="OQN13_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCS1613679.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            42881..44062
                     /gene="setB"
                     /locus_tag="OQN13_000205"
     CDS             42881..44062
                     /gene="setB"
                     /locus_tag="OQN13_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCS1613680.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(44059..44313)
                     /locus_tag="OQN13_000206"
     CDS             complement(44059..44313)
                     /locus_tag="OQN13_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCS1613681.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            44468..45040
                     /gene="yeiP"
                     /locus_tag="OQN13_000207"
     CDS             44468..45040
                     /gene="yeiP"
                     /locus_tag="OQN13_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCS1613682.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            45263..46729
                     /locus_tag="OQN13_000208"
     CDS             45263..46729
                     /locus_tag="OQN13_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS1613683.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKKHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            46847..47833
                     /gene="yeiR"
                     /locus_tag="OQN13_000209"
     CDS             46847..47833
                     /gene="yeiR"
                     /locus_tag="OQN13_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCS1613684.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            47872..48585
                     /gene="lpxT"
                     /locus_tag="OQN13_000210"
     CDS             47872..48585
                     /gene="lpxT"
                     /locus_tag="OQN13_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCS1613685.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            48997..49563
                     /gene="mepS"
                     /locus_tag="OQN13_000211"
     CDS             48997..49563
                     /gene="mepS"
                     /locus_tag="OQN13_000211"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCS1613686.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            49744..51300
                     /locus_tag="OQN13_000212"
     CDS             49744..51300
                     /locus_tag="OQN13_000212"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCS1613687.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALNINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            51382..53196
                     /locus_tag="OQN13_000213"
     CDS             51382..53196
                     /locus_tag="OQN13_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1613688.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            53197..54291
                     /locus_tag="OQN13_000214"
     CDS             53197..54291
                     /locus_tag="OQN13_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCS1613689.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            54291..55316
                     /locus_tag="OQN13_000215"
     CDS             54291..55316
                     /locus_tag="OQN13_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1613690.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            55318..56907
                     /gene="yejF"
                     /locus_tag="OQN13_000216"
     CDS             55318..56907
                     /gene="yejF"
                     /locus_tag="OQN13_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCS1613691.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(56911..57255)
                     /locus_tag="OQN13_000217"
     CDS             complement(56911..57255)
                     /locus_tag="OQN13_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1613692.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(57588..58708)
                     /locus_tag="OQN13_000218"
                     /pseudo
     CDS             complement(57588..58708)
                     /locus_tag="OQN13_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(58736..59431)
                     /gene="rsuA"
                     /locus_tag="OQN13_000219"
     CDS             complement(58736..59431)
                     /gene="rsuA"
                     /locus_tag="OQN13_000219"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCS1613693.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            59581..61341
                     /locus_tag="OQN13_000220"
     CDS             59581..61341
                     /locus_tag="OQN13_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCS1613694.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            61466..61750
                     /gene="rplY"
                     /locus_tag="OQN13_000221"
     CDS             61466..61750
                     /gene="rplY"
                     /locus_tag="OQN13_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCS1613695.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(61889..62896)
                     /gene="yejK"
                     /locus_tag="OQN13_000222"
     CDS             complement(61889..62896)
                     /gene="yejK"
                     /locus_tag="OQN13_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCS1613696.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            63078..63305
                     /locus_tag="OQN13_000223"
     CDS             63078..63305
                     /locus_tag="OQN13_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCS1613697.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            63325..65085
                     /gene="pbgA"
                     /locus_tag="OQN13_000224"
     CDS             63325..65085
                     /gene="pbgA"
                     /locus_tag="OQN13_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCS1613698.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            65160..65236
                     /locus_tag="OQN13_000225"
     tRNA            65160..65236
                     /locus_tag="OQN13_000225"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:65194..65196,aa:Pro,seq:ggg)
     gene            complement(65339..67338)
                     /gene="yejO"
                     /locus_tag="OQN13_000226"
                     /pseudo
     CDS             complement(65339..67338)
                     /gene="yejO"
                     /locus_tag="OQN13_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        16486 a        17620 c        16528 g        16704 t
ORIGIN      
        1 cggctttcgg cattggtatc caccgggtca gactctttcg gccccaaatg gctgtatggg
       61 tcgttaaaca atcccatatc atatttaacg ttcagtacat ggcgggcagc atcgtccagc
      121 tcttccatcg tcactttgcc ggatttgatc aacccaggca gatacttcga gtaatactcg
      181 tcgctcatac tcatgttgat tccggatttc agcgccacgc gcaccgcatc ttccgggtct
      241 gccgccgtgc catgtttaat cagctctttg attgcaccgt gatcggaaac ggtgatgcct
      301 ttaaagcccc actggtcgcg cagaacatct ttcagcagcc aggaatcgga ggtggctggc
      361 gtgccgttca gcgagttcag cgccaccatc accgcgccgc tgcctgcgtc cagccccgct
      421 ttgtacggcg gcatataatc attaaacagg cgctgcggac tcatatcgac ggtgttgtac
      481 tctttaccgc cttctaccgc gccgtatgcg gcaaagtgtt tgacgctggt catcaccgag
      541 tagcgatctg ccgggctttt accctgcatc gcttccacca tggttttacc cattgttgag
      601 gtgagatacg tatcttcgcc aaaaccttcg gaagcacgtc cccagcgcgg atcgcgcgag
      661 acatcgacca tcggtgccca ggtcatattc aggccatcat ctgccgcttc ataagcagag
      721 acacgtccga ccgttttcac tgcatcgagg ttaaaagacg aggccagacc gaggctaatc
      781 gggaacaccg tgcgctgacc gtgcagcacg tcgtaagcaa agaaaagagg aattttcagg
      841 cggcttaatt ccatcacctg atcctgcatg gcgcggatat cctgacgggt tacggtgttg
      901 aaaatcgccc caacctgacc gtctttgatc atctcgcgga tcgcctcttt cgggttatcc
      961 ggaccgacgc tgattaagcg cagctgacca attttctcat caactgtcat tttcttaagc
     1021 agttcggtga cgaacgcatc ccgcgcttcg ggcgttaatg gatggttgcc gaataaatca
     1081 tccgccagtg ctggctgcag ggccagactc accgcgattc ctactgaaca tagccatttc
     1141 atatggattt ttcttcctct gttgcccgac gaggcagcga aaatggtgca aaaaccgtag
     1201 tttgccataa gcatgatgga gagagaaaaa gaatgctcag tttattgtct gaattttcaa
     1261 aatattcact cgctgaattg tcatacaagg cgctattcta gtttgtgata ttttttcgcc
     1321 accacaagga gtggaaaatg tcttccatga caacaactga taataaagcc tttttgaatg
     1381 aacttgcccg tctggtcggt cattcacacc tgctcaccga tcccgcaaaa acggcccgct
     1441 atcgcaaggg cttccgttct ggtcagggcg acgcgctggc tgtcgttttc cctggctcac
     1501 tactggaatt gtggcgggtg ctgaaagcct gtgtcaccgc cgacaaaatt attctgatgc
     1561 aggctgccaa tacaggcctg accgaaggat cgacgccaaa cggtaacgac tatgatcgcg
     1621 atatcgtgat catcagcacc ctgcgtctcg acaagctgca cgttcttggc aagggcgaac
     1681 aggtgctggc ctatccgggc accacgctct attcgctgga aaaagccctc aaaccgctgg
     1741 gacgcgaacc gcactcagtg attggatcat cgtgtatagg cgcatcggtc atcggcggta
     1801 tttgtaacaa ctccggcggc tcgctggtgc aacgtggccc ggcgtatacc gaaatgtcgt
     1861 tattcgcgcg tataaatgaa gacggcaaac tgacgctggt gaaccatctg gggattgatc
     1921 tgggcgaaac gccggagcag atccttagca agctggatga tgatcgcatc aaagatgacg
     1981 atgtgcgtca cgatggtcgt cacgcccacg attatgacta tgtccaccgc gttcgtgata
     2041 ttgaagccga cacgcccgca cgttataacg ccgatcctga tcggttattt gaatcttctg
     2101 gttgcgccgg gaagctggcg gtctttgctg tacgtctaga taccttcgaa gcggaaaaaa
     2161 atcagcaggt gttttatatc ggcaccaacc agccggaagt gctgaccgaa atccgccgtc
     2221 atattctggc taacttcgaa aatctgccgg ttgccgggga atatatacac cgggatatct
     2281 acgatattgc ggaaaaatac ggcaaagaca ccttcctgat gattgataag ttaggcaccg
     2341 acaagatgcc gttcttcttt aatctcaagg gacgcaccga tgcgatgctg gagaaagtga
     2401 aattcttccg tccgcatttt actgaccgtg cgatgcaaaa attcggtcac ctgttcccca
     2461 gccatttacc gccgcgcatg aaaaactggc gcgataaata cgagcatcat ctgctgttaa
     2521 aaatggcggg cgatggcgtg ggcgaagcca aatcgtggct ggtggattat ttcaaacagg
     2581 ccgaaggcga tttctttgtc tgtacgccgg aggaaggcag caaagcgttt ttacaccgtt
     2641 tcgccgctgc gggcgcagca attcgttatc aggcggtgca ttccgatgaa gtcgaagaca
     2701 ttctggcgtt ggatatcgct ctgcggcgta acgacaccga gtggtatgag catttaccgc
     2761 cggagatcga cagccagctg gtgcacaagc tctattacgg ccattttatg tgctatgtct
     2821 tccatcagga ttacatagtg aaaaaaggcg tggatgtgca tgcgttgaaa gaacagatgc
     2881 tggaactgct acagcagcgt ggcgcgcaat accctgccga gcataacgtc ggtcatttgt
     2941 ataaagcacc ggagacgttg cagaagtttt atcgcgagaa cgatccgacc aacagtatga
     3001 atccggggat cggtaaaacc agtaagcgga aaaactggca ggaagtggag taaaaattac
     3061 ggatggcata gtatcgccat ccgtcattca cttaatcgtt ctgtgccgtc tgccccgccg
     3121 ccgccatttg ggcggctttt tgttttttat agctcaacgc tgctgctgga acaagcatca
     3181 ctttaccagt ttcaatccag gtacgcaggc ggctggcatc ggcaaaatgg gtatatttgc
     3241 caaacgcatc catcactacc agcgctaccg gtttattatt gataaccgta cgcatcacca
     3301 gacaatggcc cgccgcattg gtaaaaccgg ttttggttaa ctgaatattc cagttatcgc
     3361 gatacaccag atggttagta ttgcggaacg gcagcgtata cgtcgggtta gagaaggttg
     3421 ccatatcttc ccgggtagta cttaactgcc cgatcaacgg atattgtttg ctggcaatga
     3481 gcagtttggt taagtcacgg gcagttgaaa cgttatgcac cgacaatccg gtaggttcaa
     3541 caaagcgcgt gttgttcatt ccgagcgatt tcgctttcgc attcattgcc ttaataaagg
     3601 ctttgtaacc accgggataa tggtgtgcaa ggcttgccgc cgcgcggttt tctgaagaca
     3661 tcagcgccag caacagcata tctttacggc tgatttcgct attcagtcgt acgcgcgaat
     3721 agaccccttt catctccggc gtctggctga tatccacttt tagtttttca tccagcggca
     3781 gtcgtgcatc cagcacaacc atcgcggtca ttaatttgct gatagacgca atcggacgca
     3841 ccagatccgg gtggttcgag tagatcactt tgttggtatt cagatcaaca atcatcgcgc
     3901 taccggaggc aatttcaggt tgtgaagcgg tggtagcggc tgccgttttc gcaacggcct
     3961 gcggtgccaa aggcacagcc agcatcaggg ccaggctaaa taaagaaact cgaaatttcg
     4021 gcatgatgag cattcagata gtggttcacg cgcacaggtt gcgcaccgcc ggagtaagga
     4081 tttactgagg ctagcgacgc catcataacg agcaaaaagt gcgatcgtca aaggagaatc
     4141 gtgaggaaat gctgcattgc tgacatttac gccagcaatg caacgtcaaa gaactttcta
     4201 gaacaaacga taaccgtagc cccatagtat aacggttagg gcgagcagca cttccagtac
     4261 cagcacgcca atcgccagcg tcgaactaga gaagctaagg ccttcctctt tgttgatatt
     4321 caggaagctc ggaataccaa ggtaaagcag gtagccggtg taaaacagcg ccaccgtgcc
     4381 gaccagcgca cacaaccaga ccagtggata aagcgccacc agaccgctta aaaacagcgg
     4441 agttgcaacg tagcccgcga agaccatgca gtgcgcaagt gacggacgct gcggataatt
     4501 acgcgccatc caccagatga cccgccccat taccgcaacc ccagccagca taacgccata
     4561 aaacaggaca gccagcgcca gtccggtaaa ccaggataac ttcaggatag tgccatcgcc
     4621 aaaattccag ccaatctgtg tagtgccaat gaaggcgcaa atcaccggaa tcgccgccat
     4681 cagcaaaacg tggtgggtgt aatggtgaga aatcgtttcg ttttcgcgat taatcacctg
     4741 catttcacga tcgggatggg aaaacagtcc ccagacatgg ctcataccgc ccccttgttg
     4801 tgagttcatg aacctgacag ttcaagtata agtcagcttg tgattatttt ttgttcgctc
     4861 atgtaatttg caccgtctga tgtacgtcct ttttcagcgt gaatgatttc cagggtgtat
     4921 gcttacaggc aaccaaaggg agacagactg gcctatggat ctcaatacac tcatctcaca
     4981 atatggttat gccgcgctgg tgatcggtag cctggcggaa ggtgaaaccg tgactttgct
     5041 gggaggcgtt gcggcacatc aggggctatt aaagttcccg ctggtggtac tttctgtggc
     5101 gcttggcggc atgattggcg accaggtgct ttatctgtgc gggcggcggt ttggcggcaa
     5161 gctgttacgc cgtttctcga aacatcagga taaaattgag cgggcgcaga aacttatcca
     5221 acgccatccg tatctgtttg tcattggtac gcgctttatg tatggctttc gggtgattgg
     5281 cccgacgctg attggtgcca gccagctgcc gccgaaaatc tttctgccgc tgaatattct
     5341 cggcgcattt gcctgggcgt tgatttttac cactattggt tacgctggtg gtcaggtgat
     5401 tgcgccgtgg ttgcacaatc tcgaccagca tttgaagcac tgggtctggt tgattctggt
     5461 cgtggttctg gtggtgggcg tgcgctggtg gctgaaacgg cgcgggaaga aaaagccgga
     5521 tcatcaggcg taaaaccatt gccctgggta aggcgttcgc gccgcatccg acatccggtg
     5581 caccatgcct gatgcgacgc ttgtgcgtct tatcatgcct accaaaattt tacccattga
     5641 aataaccacg cacctcactg cgctattctg ggttgaactg cggattcgcc aacataaagc
     5701 cgccatccac tatcaacgac tgcccggtgg tgtaatttgc gccctctgag cacagccacg
     5761 ctaccaggct ggcaatctca tgcgttgcgc caaaacgccg cagaggaatc gaaggctccg
     5821 cgtcgggctt cacgtcgctg tcatccatgc cattcattgg cgtggcgatc gccccaggcg
     5881 cgactgcgtt caccaaaatc ttatgcctga ccagctccag cgccatcgtt ttggttaacc
     5941 cgccgagcgc atgtttagcg gctgtgtaag cgctggcatc cggcagcggc gtatgttcat
     6001 gtaccgacgt aatgttgatg atacgaccgc cctgcccttg tttcaccatc tggcgggccg
     6061 caatttgtga gcataaaaat gcaccatcga catcaacggt aaaaatcttg cgccactcat
     6121 cgaaagccat atcaagaaac ggcgctttgg tcattgcgcc cgcattattc accagcacat
     6181 caatacgccc cagccgttga atgagtttct ccagcgcctg cgcaccttct ggcagtttac
     6241 cgagctccag ctgcacgatc tccgcacgta cgccgtggct aactacctca cgcgcggtat
     6301 cttttgcccc ttcttcatct gagtgccagg taatgccaat atcaaacccc tgctgcgcca
     6361 gtaataacgc gcactctttg ccgatccccg aatcggaggc ggtaataatc gcaacctgtg
     6421 ccatcaagtt ctccacttaa cgctgaataa acgttaagta tagaaggcgc atatcatcag
     6481 cgtttgtaac ccccgcccaa cgcaccagtg agttgaatag aagcatccag ccactgccct
     6541 tgcaataaca ggccattggc ccgctcacgc agcgcgggga ttctggcttc gctgacgcgg
     6601 gaaccaacaa tgatgcccgc gttaaagcgc gcctgcgcaa gacctaccac acgcaaggca
     6661 tcgcgctcaa tttgcgcctg atgctggttt ttctccgcaa gtgtctgaac ctgactggct
     6721 gcccgcgcca cgtcattcac cgcttcaacc accgctttgt tgtagctggc gatagacaag
     6781 ttgctttcgg cttttgcgat attgagattg gcgttaagac gaccactatc gaaaatgggt
     6841 agcgtcaggc ctgccgtaac gcccatttgc tgcgcggaat gacggaacag atcgcttaag
     6901 tgcaacgcat cctgttgcag gaaggccatc aggttgatgt caggataaaa tgccgctttt
     6961 gccgcatcaa tggtgcttag cgatgactca acgtaccagt gcgccgcctg caaatctgcc
     7021 cgccgggcca gtaaggagta ccccagttca tcaggaagct ggcttgccac tttcggcaac
     7081 gcgaccggtt taagcttcaa tgactttgtc tggttatttg taagtgcgct taaccgtgcc
     7141 tcaataattt tcattttccc cgcgacatcg ttgagctgct gccgggtttt gctggcatta
     7201 atatcggttt ccacaccttc aactgaagaa gtaatcccgt tctgatatag ctggcgatcg
     7261 gtcgcgataa tggtgttctg ctctttttct atttgctgca agaccgtgtt taacgccgcc
     7321 tgggtttgcc actcccagta caggcgggct acgctgccag ccagcaattg gcgggtttgc
     7381 tcgcgttccg ccgcccgtgc tttaaccgta cccaggcggg cagtaacctc cgcccgattc
     7441 tttccccaga tatcgagatg ccagcccgcc gttaagccaa aagtaccgtt ggtgtaccac
     7501 gggccggtcg tacctgcggc cggatcgttc agagcaaacg gccccattaa gccttctgcc
     7561 gacatttttt gccgctccat atccgccgaa aagtcgatct gcggaccatc ctgagtggca
     7621 actgccttcg cctgggcttc agctagctga atgcgctgtt cagccacctg catatccggt
     7681 gcgttctgta gtgcattgtt aattaatgaa gtgagctgat tatcgtgata ctccagccac
     7741 cattggctgt ctggccaacc atttttcagc gccgtgggta atgcggtgtc aacttgtgca
     7801 gcgggcgttt gctggcttaa cgcctggcgg gtttcatgca taggcgcaca cccggccagc
     7861 atcagtaaca gcggaaaaca ggcgatggct ggataaaagg aatcacgatt catgggggaa
     7921 taatcaggta agaaacggtg cgcggagatt accgtgtgtt gcgatatatt ttttagtttc
     7981 gcgtggcaat acatcagtag caataaaacg acatacccag aaaaatatag actaagtaaa
     8041 tgatatcttc cgatttatct taatcgttta tggataacgg caaagggctt cgttttttcc
     8101 tatacttatt cagcactcac aaataaagga acgccaatga aaattatact ctgggctgta
     8161 ttgattattt tcctgattgg gctactggtg gtgactggcg tatttaagat gatattttaa
     8221 aattaattaa tgtcatcagg tccgaaaata acgagaatat ttcagtctct catcctgttg
     8281 cgctcctgtc atgtgcattg cttcatataa tcactggcgc aaggagcgcg cagggggcgg
     8341 ccaatcgccg ccgccccctg cacccccggg ctctggcgaa caaaatcgcc gctgcgcggt
     8401 gccctcggct tatcccttac ggctaccggg tcgggcgcga ggtaacatcc ctgtaaaacg
     8461 cgccctcagc ccacctccat gtgggctgcc ccggccttca gggaacgcct cggcaatttt
     8521 gacgccacca aacaaccgtg cggcctattg ataaagagct aacacattgt caaaaaacat
     8581 cactatggtt ttttagagtt tctcgatatc aattgcctga atagcccttg caatatcagg
     8641 ggaattattc aacacccgaa catgctgaaa taattccgtt gcttcatcgt attctttacg
     8701 caaataactc aaccactgtt taatccgcgc aacgtgatat aacccggtat cgccctgctt
     8761 ttccagacgg gtatattttt gcagcaaagc aaccacctcc ggccacggca ttcgcggttc
     8821 gttatatttt actacccggc tcaggttggg aatattgagc gccccgcgac caatcatcac
     8881 tgcgtcgcag ccgctgatcg caatgcattg ttgcgcgctc tgccagtccc agatttcgcc
     8941 gttggcaatc accggaatat tcagccgctg gcgaatctcg ccaatcgcct gccagtcaat
     9001 atgctccgcg cggtaaccct gctcttttgt ccgtccatgc accaccagct ccgtagcacc
     9061 agcctgttga accgcatcgg cgatttcaaa tttcttctca ccgctgtccc agcccagacg
     9121 cactttcacg ctgacgggca aatgcgccgg tacagcttca cgcatcgctt ttgcaccctg
     9181 gtagatgagt tcaggatctt tgagtaacgt cgccccgccg ccgctgccgt taaccgtttt
     9241 cgacgggcag ccgcaattga gatccacgcc ccaggaacct aattccaccg cacgggcggc
     9301 gttctctgcc agccattgtg ggaactgacc taacaactga acgcgcacca gcgtaccgga
     9361 tggtgtccgg ctggcgtttt gtagctcagg gcaaatgcga tgaaagactt ttaccggcag
     9421 cagttgatcc accacgcgga caaactcggt gatgcacaga tcgtagtcgt taacttcggt
     9481 cagcaattct cgcaccagag agtcaagcac accctccatc ggagccagta acacacgcat
     9541 atcttcaccc ggaaaaaaat gaggcgctat gttagcgcct atggtcaacg gattaaaggt
     9601 ctgcaaagag ggattgctgc aaactgtaat ccctctctat gcttgatgta tgcctgagtt
     9661 cgttgcgcat tgcgtaatgc cggggagcga aaaaataagc gctgatttct taatgtgatc
     9721 ggtagcacgt tttacgaatt aattgtatga tgaatccatc tcatctgggg tgttgattat
     9781 gagtaagaca ctgaacatta tctggcaata tttacgcgct ttcgtcctga tttatgcctg
     9841 cctgtatgca ggcattttca ttgcttccct gctaccggta accattccgg gcagcatcat
     9901 cgggatgctg atcctgtttg tcctgctggc cttgcaaatt cttccggcaa aatgggtcaa
     9961 tccggggtgc tacgtactga ttcgctatat ggcgctattg tttgtgccga ttggcgtagg
    10021 cgtcatgcaa tattttgatt tgctccgcgc acagtttggc ccggtagtgg tttcctgtgc
    10081 agtcagtacg ctggtggttt ttctggtggt gagctggagt tcacaactgg tacacggtga
    10141 acgtaaagtc gtaggtcaga aaggatcaga agaatgatgg cgaatatctg gtggtcatta
    10201 ccgctgacgt tgattgtctt ttttgccgcc cgcaaactgg cggcacggta taagtttccc
    10261 ctgcttaacc cgttgctggt agcaatggtg gtgatcattc cttttttaat gctgaccggc
    10321 atctcttacg acagctactt taaaggtagc gaagtgctaa acgatctgct gcaaccggcg
    10381 gtcgtcgcgc tggcctatcc tttatatgag cagctacacc agatccgcgc gcgctggaaa
    10441 tcgatcatca ccatctgttt tatcggcagc gtggttgcga tggtgacggg gacttccgtg
    10501 gcattgttga tgggcgcttc accggaaatc gccgcgtcaa tcctgccgaa atcagtcacc
    10561 acgcctattg caatggcggt tggcggcagt attggcggta ttccggcaat cagcgcagtt
    10621 tgcgtgattt tcgtcggcat cctcggcgcg gtatttggcc atacattgct taatgcgatg
    10681 cgtattcgta ccaaagctgc gcgcggtctg gcaatgggga cagcctcgca cgccctcggt
    10741 acggcgcgct gcgccgagct ggattatcag gaaggtgcat ttagttcgct ggcgctggtg
    10801 ttatgcggga taattacttc gctgatcgca ccgttccttt tcccgattat tctggcagta
    10861 atgggctaaa atttgcgatg cgtcgcgcat ttttgatgta agtttcacgc gttgcataat
    10921 taatgagatt cagatcacat ataaagccat aacgggttcg taaactgtta tcccattaca
    10981 tgattatgag gcaacgccat gcatccacgt tttcaaaccg cttttgccca acttgcggat
    11041 aacttgcaat ctgcactgga acctattctg gcagacaagt acttccccgc tttgttgacc
    11101 ggggagcaag tctcatcgct gaagagcgca acggggctgg acgaagacgc gctggcattc
    11161 gcactacttc cactggcggc ggcctgtgcg cgtacgccat tgtcgaattt taatgttggc
    11221 gcaattgcgc gcggtgtgag cggaacctgg tatttcggtg ccaatatgga atttattggt
    11281 gcaacaatgc agcaaaccgt tcatgccgaa caaagcgcga tcagccacgc ctggttgagt
    11341 ggtgaaaaag cgcttgcagc catcaccgtt aactatacgc cttgtggtca ctgccgtcag
    11401 tttatgaatg aactgaacag cggtctggat ctgcgtattc atctgccggg ccgcgaggca
    11461 cacgcgctgc gtgactatct gccggatgcc tttgggccga aagatctgga gattaaaacg
    11521 ctgctgatgg acgaacagga tcacggctat gcgctgacgg gtgatgcgct ttctcaggca
    11581 gcgattgcgg cggcaaaccg ttcgcacatg ccttacagta agtcgccaag cggtgtcgcg
    11641 ctggaatgta aagacggtcg tattttcagc ggcagctacg ctgaaaacgc cgcattcaac
    11701 ccgactctgc caccgttgca gggggcgtta attctgttga atctcaaggg ttatgattac
    11761 ccggatatcc agcgcgcggt tctggcagaa aaagccgatg cgccgttgat tcagtgggat
    11821 gccacctccg caacgctgaa agctctcggc tgtcacagta tcgaccgcgt gcttctcgct
    11881 taagcctggt gccggatgcg gcgtgaacgc cttgtccggc ttgccagccc tctcttggtg
    11941 tcgaaattcc cggcaaacag tttgccgttt cttgcgcaaa accagcgggt aaagtagcct
    12001 gatggaaatt ttccttagat cgagtctcct gcatgttaaa gcgcgtgttc ctcagcctgt
    12061 tagtcctgat cggcttgctg ctgttgactg tgctcggcct cgatcgctgg atgagctgga
    12121 aaaccgcgcc ttatatctac gacgaattgc aggatctccc ctaccgccag gtcggcgtgg
    12181 tgctcggcac agcaaaatat tatcgtactg gcgtaattaa tcagtattat cgctaccgca
    12241 ttcaaggagc gattaatgcc tataacagcg gtaaggtaaa ttatctatta ctgagcggcg
    12301 ataacgcatt gcaaagttat aatgagccga tgaccatgcg caaagattta atcgctgctg
    12361 gtgtcgaccc atcagatatt gttctcgatt acgcaggctt tcgtacgctg gattccatcg
    12421 tgcgtacacg caaagtgttc gatactaatg atttcattat tatcacccaa cgtttccact
    12481 gtgagcgggc attatttatt gcgctgcata tggggattca ggctcagtgt tatgccgtac
    12541 cgtcaccgaa agatatgctg tcagtacgta ttcgcgaatt tgccgcccgt ttcggtgcgc
    12601 tggctgacct ttatattttt agacgtgaac cgcgtttttt agggccgctg gtccctattc
    12661 cggctatgca ccaggtgccg gaagatgcgc aggggtatcc cgccgtcaca cccgaacagt
    12721 tacttgaatt acaaaagaaa caaggaaagt agttatggat gtacagcagt tttttgtcgt
    12781 tgccgttttt ttccttatcc cgatattttg tttccgcgaa gcatggaaag gctggcgcgc
    12841 aggcgcgatt gataaacggg ttaaaaatgc accggaaccg gtgtatgtct ggcgagcaaa
    12901 aaatcccgga ctctttttcg cttatatggt ggcatatatc ggcttcggaa ttttatctat
    12961 cggcatgatt gtttatctta ttttctatcg ttaatatttt ctcttttctg ttaattattg
    13021 agaataatta ttacttcact caataagctg cggatataac attctgaacc gcagctattt
    13081 gtgaatcctt tcacagttta aatttcccca cacgcttagc cttaatatca gcacgcaata
    13141 tttatttagc gttcgcctta attactcata gcattaagga agatcacatg ccgcaacaaa
    13201 attatctgga tgaactcact ccggctttta cgcctttact ggcgattaaa gaagcctctc
    13261 gctgtttatt atgtcatgac gctccctgta gtcaggcttg cccggcacag accgatccgg
    13321 ggaaatttat tcgctcaatc tactttcgta attttaaagg cgctgccgag acaattcgcg
    13381 aaaataatgc cctcggtgcc gtttgtgcca gagtgtgccc gacggagaaa ttatgccaaa
    13441 gcggttgtac ccgtgccggt gttgatgctc ccattgatat cggccgctta cagcgttttg
    13501 ttactgattt tgaacaacaa accgggatgg aaatttatca gcccggtact aaaacgctcg
    13561 gcaaagtcgc gattattggc gcaggtcctg ccggattgca ggccagtgtg acactgacaa
    13621 accagggtta tgacgtcacg atctatgaga aagaagcaca acccggtggc tgattgcgta
    13681 acggcattcc gcaattccgg ttaccgcaat cagtactgga tgcagagatc gcccgtattg
    13741 aaaaaatggg cgtgaccatt aagtgcaaca acgaagtcgg taacacactc acccttgagc
    13801 agctgaaagc agaaaaccgc gcggtactgg tcaccgtggg gttatcaagc ggttccgggc
    13861 taccgctgtt tgagcatagt gacgttgaga ttgccgtcga cttcttgcaa cgtgcacgac
    13921 aggcgcaagg cgatatcagc attccacaaa gcgcattaat tatcggcggc ggtgatgtcg
    13981 cgatggacgt agccagcacg ctgaaagttc tcggctgtca ggcggtaact tgcgtagcgc
    14041 gtgaagagtt agatgagttt ccggcaagcg aaaaagagtt taccagcgcc cgggagctgg
    14101 gcgtttcgat catcgatgga ttcacgccag tagccgtgga aggcaataaa gtcacgttta
    14161 agcatgtacg gttatcgggc gaactgacga tggcggcaga taaaattatt ctcgccgtcg
    14221 gtcagcatgc cagactagat gcctttgcgg agttagagcc gcagcgtaac accatcaaaa
    14281 cacaaaatta ccagacccgc gacccgcaag tctttgctgc tggcgatatt gttgagggag
    14341 acaaaaccgt ggtctatgcc gtgaaaaccg ggaaagaagc cgccgaggcg attcatcact
    14401 atttagaggg agcttgctca tgttaacgaa agatctttcg attacttttt gcggcgtgaa
    14461 gtttcccaac ccgttctgcc tctcttcttc gccggtaggc aactgctatg agatgtgtgc
    14521 caaagcctac gacacaggtt ggggcggtgt ggtgtttaaa acgatcggct tttttatcgc
    14581 caacgaagtc tcgccgcgtt ttgatcatct ggtgaaagaa gataccggtt ttatcggctt
    14641 caaaaatatg gagcagattg ccgagcatcc gctggaggag aatctggccg ccctgcgtcg
    14701 gctgaaagaa gattacccgg acaaagtatt gatcgcttcg atcatggggg aaaatgagca
    14761 gcaatgggag gagctggcgc gcctggtgca agaagctggc gcggatatga tcgagtgtaa
    14821 cttctcctgt ccgcaaatga cttctcatgc gatgggtagc gatgtcgggc aaagcccgga
    14881 gctggtagaa aaatattgtc gggcagtgaa acggggttcc acgctgccaa tgctggcgaa
    14941 gatgacgccg aatatcggtg atatgtgcga agtggcgctg gcggcgaagc gcggcggcgc
    15001 agatggcatt gcggcgatta acaccgttaa atccatcacc aatatcgatc ttaatcagaa
    15061 aatcggtatg ccgatcgtta acggaaaatc aagtatttcc ggatattccg gtaaagcggt
    15121 aaaaccgatc gccctgcgct tcattcagca aatgcgcacc catccagaac tgcgcgattt
    15181 cccaatcagc ggtatcggcg gcattgaaac ctgggaggat gcggcggagt ttttattgct
    15241 cggcgcagca acgttacagg tcaccaccgg cattatgcag tacggctatc gcatcgtaga
    15301 agatatggcg agcgggttat cgcattatct cgccgatcag ggatttgatt cgttgcagga
    15361 gatggtaggt ctggcgaata acaatattgt cccggcggaa gatttagacc gcagttatat
    15421 tgtctatccc cgtatcaatc ttgacaaatg tgttggctgt ggacgctgtt atatttcctg
    15481 ttacgacggc ggtcaccagg cgatggaatg gagcgagaaa acccgcacac cgcattgtaa
    15541 taccgagaaa tgtgtgggtt gtctgctttg tggtcacgtc tgcccggtgg gttgtattga
    15601 gctcggggaa gtgaagttta agaaaggcga gaaagaacac ccggtaacgt tgtagaaccc
    15661 gcgtcacatc cggcatttgg ttcctgttgc cggatgcggc gtgaacgcct tatccggcct
    15721 acaaaaccac acagatcatg atgttatgtg cgtttatgac cgaatgcgga ccacattcac
    15781 atcatttctt acgcgcgtat ttcagtgaat ccagcgctac ggcgaagata ataatcgccc
    15841 ctttgatgat gtactgccag tatgggttta cgccgatata cgtcaggcca tagttgatga
    15901 cggtaaaaat aatcaccccg gtcaccacgc caatcaccgt ccccacaccg ccgctgaacg
    15961 atacaccgcc taccacgcac gccgcgatag catccagctc atacataaag ccgaggttgt
    16021 tggtggcaga gccgatacgt ccggcttcta acatcccgcc aaaggcatag aacacgccag
    16081 acaacgcgta gatcatcagc aggttcaggc cgacgttgac accagatact tttgccgctt
    16141 ccgggttacc gccaatggca aaaatgttct taccgaagcg ggttttgttc cacaacaccc
    16201 agacgaacgc caccgcaatc aacgcgtaga aggtgatgta agagagacgg aaactcccca
    16261 gcgcgacaaa gccctgagca aaggtagaga agccactgtc aaaaccagaa attggcgacg
    16321 ccccgacaaa gtcgtaatag agcgagttga tgccatagac gatgatcatc gtgccgagcg
    16381 tggtaatgaa cggcgtcacg ttgagataag cgataatcag accgttgatc aaaccgatca
    16441 ccgcaccaat ggcacagaca atcagaataa ccagcgcaat cggcatcgtc gccatttccg
    16501 ggaacacttt gttggcgtta tccatggact gcaataatgt cgcagccacc actgccgcca
    16561 gccctacctg acgaccagca gaaagatcgg tcccctgggt gacaattaac cctgccacac
    16621 cgagcgcgat aataatacgc accgatgact gggtgagaat attacttaag ttcaacagac
    16681 ttaaaaatgt tgggtcctgg aaaataataa tcgccagcaa aactaaaaga acgacgtaaa
    16741 taccgccctc tttcaggtaa gtaagaaaac ttttcttatt taacgcactc atgggaagcc
    16801 cctaatctta aaggtgcaaa gacgcaagac gcagaatttc gttttgcgtt gttgtttttg
    16861 tatcgacaat tccggaaacg agaccattgc tcatgaccag aatacggtct gttatcccta
    16921 acaactcagg catttcagag gagataataa taatcccctt gcctttcttc gccagttcgg
    16981 caattaactg ataaatttca aacttcgccc cgacatcaat accgcgcgtc ggttcatcga
    17041 gcattaatat ttctggttgc gttagtagcc agcgaccaat aatcaccttt tgctgattac
    17101 caccggagag cgaaccaatt tgcgtccgat gacccggcgt ttttacccgc atcgaatcaa
    17161 tcacccactg ggtatcgctt ttcatccgcg agttatccag taaaccaact ttatttttgt
    17221 agttgcgaat attggaaatt aacgagttaa aaccaatatc cagataggca taaattcccg
    17281 ttgagcggcg ctcctcagtt accagtgcaa atccgtggtt tatggcttcg ttggcattat
    17341 ggttattgat ctgtttgccg tgcaacgtaa tggtgccagc cgatttctcg cgaataccaa
    17401 ataacgtctc aacaatatcg gtacgtttcg cccccaccag cccggcaata ccgaggatct
    17461 cccctttatg cagatcaaac gagacatcgc gaatcgacgg ctggcgcagt gacgttaggt
    17521 tacgcacctc gaggatgact tcgcccggct tgttttcttt gtcaggaaaa cgctgattaa
    17581 gagaacgccc aaccatcatg gcgatgatct tgtccatcgt cagtcctgcc agcggttcgg
    17641 tggcgatcca ctgaccgtcg cgcaatacgg taacttcatc acataactgg aagatttctt
    17701 ccattttatg cgagatataa acaataccgc agccgcgctc ttttaattta cgaataatag
    17761 tgaacagatg attgacctct ttttcggtta acgaagaagt cggttcatcc ataatcacaa
    17821 ttttcgcgtt ataggaaaac gctttggcga tttcgatcat ctgcatttgc gaaacggata
    17881 atgtgccgac gcgcgcacgc ggatcgatat caatatccag ttcatcaaaa attgctttgg
    17941 tttcgcggta cattttgtcc tgatcgacaa acatgccttt ggtgggatat cgccccagcc
    18001 acatgttatc catcaccgaa cgttgtaata ccaggtttaa ctcctggtgt accatcgaaa
    18061 taccattttc cagtgcctct ttggcggaat ggaaatcgat ctctttaccc tggaataaaa
    18121 tggtgccgga gtctttttga taaataccaa acaggcattt taataatgtc gattttcctg
    18181 caccgttttc ccccattaat gcatggatag aatgtggccg gacttttaaa ttaacgttat
    18241 caagtgcctt aacaccagga aaggacttgt tgataccgct catttccaac aagtattccc
    18301 cggaagacgg agtcgttgag ctgaccatat aattttacct tgttggccat acaataaggg
    18361 cgcagtaaac gactgcgccc aatcagtctt atttcttgct gaattcagcc aggttgtctt
    18421 tatctacgcc aacataaggt acgcggacca ctttgttgtc gattttccag ttggtgccat
    18481 cagccgcacc tttgccatcg gccaggtttt tcgccagatc aaaggtcgct ttcgcctggt
    18541 tgttagcatc gttcagtact gtgcccgcca gtgcaccgga tttcaccagc gccagcgctt
    18601 ctggcagcgc atcgacgcca aacaccggaa tgctggactt gttgtgtgct ttcaatgctt
    18661 ctaccgcgcc cattgccatc gcatcgttgt tggcgataac cacttcgatt ttgttggcgt
    18721 tcgggccaga cagccaggcg tccatcttat ctttcgcctg agcggtgtcc cacattgcgg
    18781 tatctaactg taactgttca gttttgatgc ctttatcgtt caattcttta atcacgtaag
    18841 tggtacgtgc ttctgcatcc ggatggcccg gttcaccttt cagcagtacg aactgaatct
    18901 gaccgtcttt gttcagatcc caaccctgat tcgccgccca gtgtttagca atcaaatcgc
    18961 cctgaataat gccggactct ttggagtcag tgccaacgta gtaggctttg tcgtagctat
    19021 ccagcgcctt acgagacggt tctttgttga agaaaaccac cggcacgttt tgcccacgcg
    19081 ctttctcaat caccgtaccc gcagctgccg ggtcaaccag gttgattgcc agtgccttca
    19141 cccctttcgc cagcaatacg tcgatctgat cgttctgctt ggactggtca ttctgagaat
    19201 cattcatcag cagctgaaca tctggcgcgg ctttcgcatc ttgctcaata gccttgcgca
    19261 ctacagacat aaagttatcg tcgtacttat agattgttac accaatgcga gtatcagcag
    19321 cgtgtgcagc ggcaccgaat aacatgctgg ccatcacagc agacagggtt aacaccttct
    19381 tattcatggt atctccggtt tttcttatgc agggtagtgc ttgagataaa tgctcggcgg
    19441 ggcagtagag ttaatgaagt gttactgaac gccgaagctc acttttttaa attcgttctt
    19501 ccatgcccgg taacgctcca gaaaacggct ttaattgttg ttatgacgct attacctcgg
    19561 tgaaagtgat tattcactgt tacatacggg ttacaacgtt aaaacggtgc aatcatagct
    19621 atcacattgt taagatactg tgaaatcact cacagattga aagcggttac atcgcctgat
    19681 ttgttgagtt agtgatcgcc gccgcattct ggcgcgttgc gacagaatgg cgacgcacta
    19741 acgtcggcat aaaacagtgg ctggcacgag gatcaatatt gcctgctgcc ccctgcaagg
    19801 ccagttcggt ggctaattta gccattgaag caatgggata acgcacggtc gttaattgcg
    19861 ggtcggtgta acgggcgatg ggaatatcat cgaaaccgat gattgagaga tgtaacggaa
    19921 tcgcaatgcc attatctttt aatgctgtca gtgcgccagc ggccatattg tcgttataag
    19981 caaatatagc ggtaagttgt agattgcgcc ccagcagttc aaccatcgcc gcctcaccgc
    20041 ccggcatgtc cggcgtacca gtgccaatcc agctttccgg cggaataata tcctgctctt
    20101 tcaacgcact catccagcct gctttacgca tggcgtcatc ttcaatgccg tgactggaag
    20161 aaagataacc aatacgttga tgaccgttat tcagcaacat gcgcgtcgcc attcgggcac
    20221 cgctgagatt atccaggcaa acgcaacgat gggcgtaccc cggcacaacg cggttgatta
    20281 acaccatacc gggaatgtta tccataaatt gcgccagttc atcgtcactc aatgcttttg
    20341 agtgaacaat caacgcatta caacgctggc gaattaacac ctcaatggcg tgacgctctt
    20401 tttccgcttc atgatagcta ttgccgatta gcacgtattt ctgatgctgc tgagcgacta
    20461 gatccaccgc ttttaccagc gcgccgaaaa acgcatcaga aacgtccatc accaccacgc
    20521 caatggtgtc gctaacctga gttgccagcg cctgggcatt ggcgtttggc cgataatcca
    20581 gctcactcac ggctttcatt actgcttcac gcgtgtcggc actgaccagc gtgctgttat
    20641 tgagcacccg ggaaaccgtt gccacagaga cgccagccta acgcgctaca tcacgaatgg
    20701 tgatcatatt cactacctgt tatgagattg cagtaaatga ctgcttgctg gcggctattt
    20761 tgtcagcact gaatacagga cttcgtgaat cgagtcacag caatggaaac ggttacagcc
    20821 gttttgttaa cgaatgtgat ccagatcgtt atcttgatgt tttagatatt gccgttccgg
    20881 cagcacgcaa agttaactga cgccagagcc attccaccgg cccctggcgg aagtaacgca
    20941 gccagataac agagaagagg atattcgcca gccataccgg aataacaaac gccagcagct
    21001 ccaggcggtc aaaatgcata aacaaaccga ggtggtaaaa aagcatggta caaatcagcg
    21061 tttgcaatag atagttggtc agcgccatcc gtccgacgca ggcgatcgca agcaccagct
    21121 taaagcggct caattgcggc cagaagccat aaaacagcga cgcatagcca atcgcctgaa
    21181 acggcgcact cagttcccgc ggcatttgaa gtaagaaggc acaccagcga tacgcccagt
    21241 ccagctgcca ttgcagggcg atggcaggaa ggttaatggt caccccaatc gccaccagca
    21301 caaaaccagt acgacgatag tgacgtaagc tgaactgccc tttcagccag ccgctgcgca
    21361 tcagcgccgc accaatgagc atcatccctg ccagttgcca gccatactgt gcgcccagtg
    21421 ccagtaaact gttgccaaca ccatcggcac gattactgat cgcttcaacg ccgccgtgaa
    21481 gcttccagta tttttcatac aaaatagccg atgcatccgg cgtccaggtg cggctggtct
    21541 ggctgtcgga aatcagcccc aataacagca aaacgccaag ccccaccaga taaagcatga
    21601 cgcctgtatt aaacaggctt tttaccgatg gcgcatcgcg caccagccgc cagcagatta
    21661 agcccaccag cccgtaagcc agcagaatat cgccgtccca gaacaataaa ccgtgaataa
    21721 aacccagcaa gactaacagc gttaaccgcg actggatcca gcgtctgcca cggggcagca
    21781 acatttgcag gcccgcacca aacagcagcg caaaaagcgt gaggaatttc acctggccga
    21841 tgagatcgag aaatgcccag gtccatgcat cctgcggcgt aatagcgccg taccaggcgg
    21901 gattgagata agccgccttt ggtagcccaa aggcgctgat gtttaatagc aggatcccca
    21961 gaatggcgac gccgcgaaca aaatcgagcg ttacgttgcg ctccatggtt cctgcctttt
    22021 aatcagttgt gatgacgcac agcgcgcaga aactcgtggc gcgtattctg actggatttg
    22081 aacaatccac caagagaggt cgttgttgtg gcactggttg catcgcggat gccacgcgct
    22141 ttcacgcagt aatgcaccgc atcaatggag acagccacgt tattggtgcc cagcagcgtt
    22201 tgtagcgcaa taagaatttg ctgcgtcaga cgttcctgca cctgcggacg ctgggcaaag
    22261 aactgcacga tacggttaat ttttgacaga ccgatcaccg aatctttcgg gatataagcc
    22321 accgtcgctt tgccatcgat ggtaacaaaa tggtgttcac aggtactggt cagagtgata
    22381 tcgcgcacgg tgaccatttc atcgaccttc attttgtttt caatgagggt gattttcggg
    22441 aagttggcgt aatccagacc ggagaaaatt tcatcgacat acattttagc gatgcgatgc
    22501 ggcgtttcca tcaaactgtc atcagccagg tcgagattca gcagctgcat gatttcggtc
    22561 atatgaccag caataaggct tttgcgcgtt tcgttatcca tttcatgcac gggcgggcgc
    22621 agcggtgttt ccagtcctcg cgcaactaac gcttcatgaa ccagggccgc ttctttactg
    22681 agtgatggca tttatgattt ctcctgcagg tgtgacgcct ccgccctgcg tgggggcaaa
    22741 gttattaagc tgatttacag cctgattatt gtgcgtgagg cggcgcacat aatccagtat
    22801 tcacagcgat aattattgta attgccgctg actttcatca gcagatgtta aaacatcgtt
    22861 atgcaaatac ggaagtgaaa gttactcaca gcacattgaa taaacggtat gatgaagaaa
    22921 ttgcaaacaa cacaacaagg agccacgcat ggaaatgctc gaagagcacc gctgttttga
    22981 aggctggcag caacgctggc gacacgactc cagtacctta aactgcccga tgacgttcag
    23041 tatctttctc ccaccccctc gtgatcacac cccgccacca gtgctgtact ggctttccgg
    23101 attaacctgc aatgacgaga acttcaccac caaggcgggt gcccagcgcg tggcggcaga
    23161 actggggatt gtactggtga tgccagacac cagcccgcgc ggcgaaaagg ttgccaacga
    23221 cgatggctac gatttaggcc agggcgcagg cttttatctt aatgccacgc aaccgccgtg
    23281 ggcgacgcat taccggatgt atgattatct gcgcgatgaa ttaccggcgc tggttcagtc
    23341 gcaatttaat gtcagcgacc gctgcgccat tagcggtcac tcaatgggtg gtcacggtgc
    23401 gctgattatg gcgctgaaaa atccgggtaa atacaccagc gtttcggcct ttgcgccaat
    23461 tgtgaatccg tgcagcgtcc cgtggggaat caaagcgttt agcacctatt taggtgagga
    23521 caaaaatgca tggctggaat gggatagttg cgcgctgatg tatgccagta acgcgcagga
    23581 tgcgatcccg acgcttatcg atcagggcga taatgatcag tttcttgccg accagttgca
    23641 acctgcggta ttggcagaag ccgcgcgcca gaaagcgtgg ccgatgacgc tgcgtattca
    23701 gccgggatat gatcacagtt actacttcat cgcctctttt atagaggatc acctgcgctt
    23761 ccatgcgcag tatttactga agtgaaagtc cgcccggttc gccgggcatc ttctcatcag
    23821 aagcgataat ccactgccat aaagtaacga cgtccgtctt cgttatagct gtagtcgtca
    23881 cgactgagat ccttgtcgcc aaggttcagc acgcctgcgc gcagtttgac gtctttagtc
    23941 acctgccagg ccgcgccggt attccagatg gtataaccgc ccggtgtttt agccgtcgcg
    24001 ctgtcggcgc gtttctgccc ggtatagtga ccagaaacat agaatgacca gtcttccagc
    24061 gccagcggtt tccagtccag cgtaccgtta gcagtatgga acggcagatc ggatagcggt
    24121 ttgttttcgc cgttgctgac atcacgacca tcgttgtagg tgtagttgat cgacagtttc
    24181 cattcatcgt tgaacggaat tttcagttcg gtttccacgc cctgaatacg agctttgtta
    24241 acgttgtagt agctaaatac cggtatgcgc cgtccgttag cgcccgtctc aaaaccaaca
    24301 aagttttggt agcccggtgc agcgttgacg tcagacgtac ggctgatgct gatacgatct
    24361 ttcacatcgt tacggaaaac ggtaacgctg gattcaaccc cttccagcca gccttcttca
    24421 cccatgtagt aaagccccag ctcccaactt tcgctggttt ctggtttcag atccgggcta
    24481 cccacaatct tacatgcgcc acggcaggaa ttgctcgtcc agtcagggct aagttgcaac
    24541 agagaaggtg ctttaaatgc cgtcgcccag ccccctttca ccgttacggt gtcggtggcg
    24601 ttataaacca ggtaggcacg cggactccag tgttcaccgt aggtttcgtg atcgtccata
    24661 cgcacgccgg tcgtcagcgc cagcggctcg aagatccgcc attcatcttc cacaaacagc
    24721 gcgtactggc tggcagacgt tttggagctg gttcccccgg tcaggttcac cgcatcgcta
    24781 agtttgtcgt gacgccattc accgccaacc gtgagaaact gattaatcgc cgtcagcggc
    24841 aacgtgtatt tgccgtcgac cgtattgctt tcggaagtta tcgggctgct gttgccaggg
    24901 tttttgttct cgactttctc accgtagtat ttcagttcgc tggtgccgta atcccaacgc
    24961 ccattatggc tgacggagta gttctggcgt tccaggcggt ttttgtccag cgagtcggaa
    25021 tcacgatcct gacggtcgaa accgtatccg gcagtaaaat cgtgattttg attcggtgtc
    25081 caggcaaatt cgacattgcc gtcgcggctg gagaatcctt caatacgcgg cgtttctccg
    25141 gtatcggtgg tcgttgagtt ttgcgggtca tccttttcac gttttgccag gctgccgtaa
    25201 gctttcattc ccagcacacc atcaattaat ggtccactgg taaagaactg accgttatag
    25261 gtatcaccgc gatcgcgatg ttcctgaatg gtggtatcga cggtaacggt acccgaccat
    25321 ttctgaccga tttttttggt gatgatattc actacaccgc cgagcgcatc ggaaccgtac
    25381 agcgacgaca tcgggccacg gaccacttca atacgttcga tggaatcgac cgggatccag
    25441 ttcagatcga aatcattgtg gcggaagacg gcattgcggg agttcacgcg tttaccgtcg
    25501 acgagaatca gggtatagct gctgtccaga ccacgaatac taacgccctt acggttatcc
    25561 ccttcgttcg tcagttgtac gccaggcact tctttgagga catccttcag attctgtacc
    25621 ggttttcgct gcagatcttc ctgggtaatg acgctgatac tggcgggtgc atctttaagg
    25681 ttttgttcaa cggaagatgc agtgacaacc atcgtttcgc catcgtcatc gaccgctaac
    25741 acaggccata cacaagaaat agcggacaaa cacagcccga cccgtacgaa agggttcaac
    25801 ctaaacattc catatctcca tgaggtaact acgaaaataa aatgggtatc gctcacatct
    25861 tctttacgtc cccttgcgta cggcagcatc gcggtggact tatttgtatg caggtgatca
    25921 tcccagaaag ccggaccagc ctctgatttg tcagcctttt ttgattgtag cgtaacgata
    25981 atgcaaacga taacaattat caatccaaat gttaaatttt atatccgcat gatttgtcag
    26041 ggaaatggaa ttgaaaaagc cctctcggtt gagagggctt agcaaggaag ggaggaaact
    26101 tatttcttat cgttctgcgg gaacttcatt tcgctgtagc gtacgaagtg agttccttta
    26161 atcagcttgt agccgaacca aataatcagg aacagcggga taccaatata cgttgccgct
    26221 acgccgcccc agtcaatggt atctttcagg aacgcttcgt agttctggcc caaagtgata
    26281 atcagacaca gaatgaatgc gaagatcggc cccagtggga agaaacctga acggtacggc
    26341 agatcgttaa tgtcgtgtcc ctgcaatacg taaccgcgac ggaagcgata gtggctaatg
    26401 gcaatcccca gccaggcgat aaaacccgtc atcccggagg tgttcagcag ccacaggtat
    26461 accgtctggt tgccaaacat ggaggtcagg aagcacaaac cggcaatcac cgtcgtcgca
    26521 tacagcgcat tacgcggcac gccaccacgc gacaatttag cgaaaatgcg cggcgcttta
    26581 ccgtcacacg ccagggtgta cagcatacga gtagacgcat acataccgga gttacctgct
    26641 gacagcaccg ccgtcagaat aactgcgttc atcaccgccg ccgcagagag cagacccgcg
    26701 tgctggaaca ccaaggtgaa cggactgacg ctgatgtctt taacatcgtt acgcagcagg
    26761 ctcggatcgg tatacggaat aatcaggctg ataatcagga tcgcgaacac atagaacaac
    26821 aggattcgcc agaatacctg acgtaccgcg cgtggaatgt ttttcgccgg atcttcggac
    26881 tcgcctgcag caataccgat cagctcggtt ccctggaaag agaagccgac aatcatagct
    26941 acgccgatca tcgccgcaaa accaccagca aacggcgctt cgccgattgt ccagttgctc
    27001 cagcccgcag gctgcgcgcc tttgaagata ccgataatca tcagcacgcc aacgatgata
    27061 aagacaataa ctgtcgtgac tttgatcagt gaaaaccagt attccgcttc accaaagcca
    27121 cgaactgaga tgtagttcag caggaagata acgccgagga acaacgcact ccagatccag
    27181 cccggtgtat ccgggaacca ccagctcatg accagctgtg ctgcaaccaa gtcaacggca
    27241 atagtcaccg cccagttgta ccagtagttc cagcccagcg cgaagccaaa gccttcttca
    27301 acatagttct gaccgtaagt ggcaaacgaa ccggaaaccg gcatatatgc agccaattca
    27361 ccgagactgg tcatcaggaa gtaaaccatc aggccaatca gcatatacga gagcaatgcc
    27421 ccgcccggac ctgcctgaga aatcgttgcg ccagaggcaa caaaaagacc tgtaccgatg
    27481 gaaccgccaa tggcaatcat cgtcaggtga cgcgccttta attcacggcg taagcccggc
    27541 gcttctgtag ttttagtttc ggaacccata caaaaatgct atccatctta aaaatgaggc
    27601 gcgattgtag cagacgattg gcaatccttc cggcagaaat acacggttat aagacacctt
    27661 catgatcgcc cagggattat aagtaaagca gccaatctct tttctggcga atgcatgctg
    27721 agggcgctgc gtcacgaaat cgacacagca ccagcatgtt cttgtacagc accttaacgc
    27781 ggtacattcg cgggatcgca atagtccaga aagcgccgta gcgcgttgga aaggtgtttt
    27841 tgccgatgat gtatacgcca caacgtacgc atcaggcgcg ggagagggac cgcaacttca
    27901 cttaatgtgc ctgcctgcaa ttgatcttca atcacacgtc gcgacaggca actaattccc
    27961 aacccatgac gcaccgcatg tttgattgcc tcggagttac ctaattccat cgccatctca
    28021 aacttcggta aatgtgacag caacagataa tcgacaatct cccgcgtgcc ggaaccgcgt
    28081 tcacgcagga tccacggcgc agcggccagc tgttctaagg tgaccggacc acgagccaac
    28141 ggcgaagtcg gcgcggcgaa aaccaccagc tcgtcttcca gccacggttc agaaatgatt
    28201 tcagtgctgt ggcacggtcc ttcaataaag ccaatatcaa cgcggaaatc cagcaccgct
    28261 tgcatcacgt cctggctgtt cccaacgcta agttcaatcg gcaactgcgg ataatcatgg
    28321 cgataacggg cgataactgc aggcagaatg tagttaccga tggtactact ggcatagata
    28381 cgaatcgcgc cgttgtcttc gcgaaacagt tgttcgattt caaccgcctg ttcaagcaat
    28441 gccaacgcac gcggatagag cagccgcccg tgttcattaa caaccagtct tttccccacg
    28501 cgatcaaaca gttgcacgcc aagctgccct tccaggtcgg tcaaggctgc gctcactgct
    28561 gattgcgaca acgccagcat caccgacgcc tgggtggttg atccactttt caatacttct
    28621 gcaaaaactt ccaactgccg gagggtgatg tgcatagtcg cttaccactt ataaagatta
    28681 attataaata tataatcaat tttattttta aaccagttag tcgtaacctt atgcccggtt
    28741 aaaggagagg gttatgacga atatcacctt acagaaacaa catcgtacac tgtggcattt
    28801 tattccgggg ttagccctga gtgcagttat caccggggtc gccctgtggg gtggttccat
    28861 tcccgcggtt gcgggtgccg ggtttagtgc cctcaccctc gcaatcttgt tggggatggt
    28921 tttaggcaac accatctatc cgcacatctg gaaaagctgt gacggtggtg tgctgtttgc
    28981 caagcaatat ttattacgtc tgggtatcat tctttatggc ttccgtctga cgttctcgca
    29041 aattgccgat gtcggtatca gtgggatcat cattgacgtg ttgacgctgt ccagtacctt
    29101 cctgctggct tgcttcctgg ggcagaaagt gtttggtctg gataagcaca ccagctggtt
    29161 gatcggtgcc ggtagcagta tctgtggtgc tgccgcggta ctggcgactg agtcggtagt
    29221 gaaagcggaa gccagtaaag taaccgtggc tgttgcaacc gttgttatct tcgggaccgt
    29281 cgcgattttc ctctacccgg cgatatatcc gctgatgtct caatggttta gtccggaaac
    29341 tttcggtatc tacattggtt ctactgtgca cgaagtggcg caggtggtgg cggcaggtca
    29401 tgccatcagc ccggatgcgg aaaacgcagc agttatttcc aaaatgctgc gcgtgatgat
    29461 gctggctccg ttcctcatcc tgctggcggc gcgtgttaaa cagctgtctg gggcgaacag
    29521 cggcgagaaa agcaaaatca ctattccgtg gtttgccatc ttgttcatcg tcgttgccat
    29581 ctttaactcg ttccacctgt taccgcagag cgtggtgaac atgctggtaa cgctggatac
    29641 cttcctgctg gcaatggcga tggcggcgct gggtctgacc actcacgtca gcgcgctgaa
    29701 aaaagctggg gcgaaacctc tgctgatggc actggtactg tttgcctggc tgattgttgg
    29761 tggtggtgcg attaactatg tgattcaaag cgtcatcgca taaaccacta catcttgctc
    29821 ctgttaaccc gctatcatta ccgttttcct ccagcgggtt taacaggagt cctcgcatga
    29881 aatacattgg agcgcacgtt agtgctgctg gcggtctggc aaatgccgca attcgcgccg
    29941 tcgaaatcga cgcaaccgcg tttgccttgt tcaccaaaaa ccaacgtcag tggcgtgccg
    30001 caccgctcac gacgcaaacc atcgatgaat tcaaagccgc ctgtgaaaaa tatcactaca
    30061 catcggcgca aattcttccc cacgacagtt atctgattaa cctcggacat ccggtcactg
    30121 aagctctgga aaaatcgcgc gatgccttta tagatgaaat gcagcgttgc gaacaactgg
    30181 ggctttcttt gctcaacttc caccctggca gccatctgat gcagatttca gaagaggatt
    30241 gccttgcgcg tattgccgaa tccatcaaca ttgcgctgga taaaactcaa ggtgtaacag
    30301 cggtgattga aaacaccgcc ggtcagggca gtaacttagg gtttaaattc gaacatctcg
    30361 cggcgattat cgacggcgtg gaagataaat tccgcgtcgg cgtctgcatt gatacctgcc
    30421 atgctttcgc tgccgggtat gatttgcgta ctccagccga atgcgaaaaa acgttcgctg
    30481 attttgcccg tattgtcggc tttaagtatc tgcgcgggat gcaccttaac gatgcgaaaa
    30541 gtacctttgg cagccgcgtt gaccgccatc atagcctcgg tgaaggcaat atcggtcatg
    30601 atgcgttccg ctggatcatg caagacgacc gtttcgacgg cattccgctg atccttgaaa
    30661 ccatcaaccc ggatatctgg gcagaagaga tcgcctggct gaaagcgcaa caaactgaaa
    30721 aagcggtggc ctgatcgatg aacgacaggg aaaagcagat tcttaaaatt ttacggcgta
    30781 acccgctgat tcaacagaat gaaatcgccg atattctgca aatcagccgc tcacgtgttg
    30841 ccgctcatat tatggatttg atgcgcaagg gtttgattaa aggcaaagga tatatcctta
    30901 ccgagcagga ttactgtgtg gttgtgggtg caatcaatat ggatattcgc ggaatggcgg
    30961 atatccgtta cccgcaagca gcctctcatc caggttctgt tcactgttcc gcaggcggcg
    31021 tcggacgtaa tatcgcgcat aacctggctc tgcttggtcg ggatgtgcat ttaatatcag
    31081 ctattggcaa cgatttttac ggcgagacat tactggaaga aacgcgccgc gcgggcgtga
    31141 acgtttcaaa ctgcattcgt ctgcatggac acagcactgc aacttatctc gctatcgcta
    31201 ataagcagga agaaactatc ctggcaatca atgacacaca tattctgcaa cagctaacgc
    31261 ctcaactgct caatacttca cgggacttga ttcgccatgc tggtgtggtg ctggctgatt
    31321 gtaatctcac acctgaagcc ctggagtggg tctttaccat tgctgatgaa attccgatgt
    31381 ttgtcgatac cgtttccgaa tttaaagcga acaaagtaaa aaactggtac agccggatcc
    31441 acactctgaa accaacacaa aatgaactgg aaattttgtg ggggcaaccg attaaagacg
    31501 acaacgatcg catcagagct gtaaattcgc tgcatcaaca aggtgttaaa cgcatttttg
    31561 tttatctgaa agatgaatct gtattttgca gcgataagga tggagaacaa tttttgctga
    31621 ccgcgccagc gcatacgaca gtagacagtt ttggtgctga cgatggtttt atggcgggcc
    31681 tggtatatag ctttcttgaa ggatacagtt tccgcgacag cgcccgtttt gcggtagcct
    31741 gcgcggcaat ttcgcgcgcc agcggcagct taaacaaccc taccctgtct gccgataacg
    31801 cgctttcatt agtgccaatg gtgtaacaat gttgccggat tcgacgcttg accgcatctt
    31861 atccggctca taaattgcgc actacgctaa accaataaag aatcctgcaa tagtagcact
    31921 catcaggtta gaaagtgtcg ccgccgccag cgcgcgtaaa ccaagttggg cgatttccgg
    31981 cgcacggtgt ggcgcaaccg cagaaaacgc ccccaccacc accccgatag aaccaaagtt
    32041 agcgaaaccg cacaacgcga aagaaataat cgccacggtt ttagcatcca gagtgccacc
    32101 cgtttgcaga tatggcgaga aattgagata agcgacaaat tcattgatcg ccagcttctg
    32161 cccaatcaaa ctcccggcaa gatttgcatc actccagtca acccccatca cccacgccaa
    32221 tggggccaac aggtaaccta aaatcgactc cagcgaagca tgttcaaaac caaaccagcc
    32281 gccaacaccg ccgataatac cgttaatcaa cgcaattatt gcaacaaatg ccataacaac
    32341 tgtcgctaca ccggcggcga ttttcagccc ggtcattgcc cctgtcgcag cggcttcaat
    32401 aatgcttttt ggcggtgttt cggtgaaaga gaggttatta aaagaaacct gcgaagattc
    32461 cgtagccggg cttaacaggc gggcaaacaa gatcccgcca gggatcgcca ttaacgatgc
    32521 cgccagcaaa tattccacag gtacgcccag tgcggcgtaa ccaatcattg tcgaaccagc
    32581 aatcgaggcc atgccactac aaatcgctgt aaataattca ttgcgattca gacgatcgat
    32641 aaagggtttg acgatcgccg gaatttcgtt ttgcccgagg aaaatggtgg tgaccgcgac
    32701 gaatgactcg atcttgctga tatttaaagc tttctggaag ataccgccga gaatgcgaat
    32761 taagatcccc atcacaccga tgtagtagag aatactcacc agtgcagtga cgaagataat
    32821 tgccggtaat accctgaaac caaagataaa tcctgcgcca tcaaataagg tatccatttt
    32881 cggtccgacc agagaaccga agataaatgc gctacctgcg tcgctgtacg ccatcacttt
    32941 gtggacgcca aaagcgactt tttctgcgac ccaacgccct ggcggtaacc aaagcataat
    33001 cccgccaatc acgacctgta acactaacgc cgcgccaacg gtacgcaggc tgatcttctt
    33061 cttgtttact gacagtaaaa acgcaattgc cagcagtacc accattccca gaacacttct
    33121 catgacatcc atagtgatta tctcttcatg tcattttggg cgacggcttt ctgcccatca
    33181 gaaatagccg ggcctgcatc ttgcgatact gtcccggctt gttatcaaca cttaatgggt
    33241 ttttatgtag ccgcgtacgc actcttcgac taatccccag aaccagtcgg tatcaatagt
    33301 gatgccgact ttagtattgg cgggcttgcc aagaacgccc agctcgtcgc agacggtacg
    33361 cccatagcaa gggccactgt tgacgtccac ttcgacgtac atctcctggg ttttaatgcc
    33421 atcagggtta atcagataac cgatgcaggt ggcgtcgtgc accgggccgc cagccaggcc
    33481 gtagttttcg aactgcgttt tgagagtgaa gttcatgatg tcgctgaaca tctctccggc
    33541 agggccgcct gccctttcca ttcgtgcaat tacgtccggg gtgcaaacgg tctggttggt
    33601 aagatcgagg cccatcatca ctaatggaac gccggaggtg aacaccacgc gtgcggcttc
    33661 cgggtcggca aagatattga actcggcaga tggggtgaag ttgcctgtac cgtaagcgcc
    33721 gcccattagc acaatttcac ggattttggg caggattgcg ggttgcatac gcattgccac
    33781 cgcgatattt gaaagcggac caaccggcac cagagtgata tcgccatcgc ttgccataag
    33841 ggtatcaatg atgtatttca ccgcgtgggt actttctgcc tggcgggtca gcggctcgaa
    33901 taccgggcca tccagtccgg tttcaccgtg aatattatcg gcaacgattt gttgacgcat
    33961 aatgggttgc ggcatccccg cataaaccgg aacattaatc tccagtttct ggcaaacatt
    34021 caggccatta attaatgttt tatcaagcgt ctgattaccc gcaacaatgg tgatacctaa
    34081 taaatcaatc gccggatgtt tcgccgccat cattatagca atagcatcat catgacccgg
    34141 atcacaatcc agaataattt ttctcttttc cattgtttat ttcctctgtt tccagttgcg
    34201 ttattttttc tacagcaacg aaatttcgat cgggccgatg attgaatctt aacaacagcg
    34261 tacgaatgct aaatatgaga aatctcatag cggataaaca tcgtgaaaga aatccacaat
    34321 aatgatctta agcagcaatt gatgagtgaa tccgcgttta aggattgctt tttaacggat
    34381 gtttcagccg atacgcggct gtttcatttt ttagcgcgtg actacattgt gcaggaaggg
    34441 caacagccgt cctggctgtt ttacctgacg cgaggccgcg ccaggcttta cgccacgcta
    34501 gctaatggtc gcgtgtcgct gatcgatttc tttgccgccc cctgttttat tggcgagatt
    34561 gagttaatcg acaaagacca tgaaccgcgt gcggtgcagg ctattgaaga gtgttggtgc
    34621 cttgcgctcc ctatgaaaca ttaccgcccg ctgttattaa acgacacgct atttttacga
    34681 aaactctgcg tcaccttaag tcataaaaat tatcgtaata ttgtttcttt aactcagaat
    34741 caatcatttc cgttagtgaa tcgcctggca gcatttatat tactctcgca ggaaggtgat
    34801 ctttatcacg aaaagcatac gcaagcggca gagtatttag gcgtttctta tcgacatctt
    34861 ttatatgttc tcgcgcaatt cattcacgac ggtttattaa ttaaaagcaa gaaagggtat
    34921 ctaattaaaa acagaaagca gttgtcagga ctggcgctgg agatggaccc ggagaataaa
    34981 ttctccggga tgatgcagta aaaattattg gcaatagcac gattgccgga tgcaacgctt
    35041 aataaggcgt acatccggca atgaaactca agctaaacca ataaagaacc cggcaatggt
    35101 cgcgctcatc aggttggaaa gcgtcgccgc cgccagcgcc cgtaaaccaa gctgggcgat
    35161 ttccggcgcg cgttttggcg aaatagccga aaacgctcca acgacaacac cgatagaacc
    35221 aaagtttgca aacccacaga gggcgaacga gataatggca atggtcttga catccagcgt
    35281 gccgctcgtt tgcagatagg gcgagaaatt gagataagca acaaactcat taatcgccag
    35341 tttctgccca atcaaacttc cggcaagatt tgcatcactc cagtccaccc ccataatcca
    35401 tgccaacggg gccagcacat agccgaaaat gctttccaga gagacattgg caaaaccaaa
    35461 ccagccgcca actccaccaa taatgccgtt gatcagcgca atgatcgcta caaatgccat
    35521 taccaccgtc gccacaccgg cggcgatttt cagcccggtc attgcaccgt tcgccgcagc
    35581 ttcaataatg ctttttggcg gtgtttcgct aaacgacaga ttttcaaacg taacttgcga
    35641 aggttccgtc gccggactaa gaatacgtgc aaacaggata ccgccaggaa tagccatcag
    35701 cgaagcggct aacaaatagt caatcggcac ccccattccg gcatagccaa tcatcatcga
    35761 acccgcaata gacgccatgc cgctacaaat agccgtaaat agctcgttgc gattcatgcg
    35821 atcaataaaa ggtttaacga tcgccgggat ctcattttgc ccgaggaaaa tagtggtgac
    35881 tgcgacaaaa gattcgattt tgctgatgtt gagggctttc tgaaaaatac tgccaagaat
    35941 acgaatcagc agccccatta cgccaatgta gtacagcaag ctgataagcg cggtaacgaa
    36001 aataatcgcc gggagtacgc gaaaggcgaa gataaagccc gcaccgtcaa acagaacatc
    36061 cattttggga ccgaccagcg aaccaaaaat aaaggcactt cctgcgtcac tgtaggacat
    36121 cactttatgg acgcctaatg ccgcctgctc tactgcccat tttcccgggg gaaaatagag
    36181 catgatgcca ccaatggcaa tttgcagcag cagcgcagcg ccaaccgtgc gcaagctgat
    36241 gctcttttta tttaccgaca acacaaaggc aattgccagc aataccacca tccccgcaac
    36301 acttctcatt atatccataa tgattttccc ttcatgccag taaacccggc gtcagcgcca
    36361 ggttttggta tgtttgatga gtacgggcga cggctttctg cccgccagaa attaacccac
    36421 gagacgctga tattctttgg caatttcgct cgccagaatg gcgttattaa agaccagttg
    36481 aatattggat ttcaggctat caccgccggt tagctccgca acacgcgcca gcaggaacgg
    36541 tgtactctct ttcccaataa caccctgttc ttcggcttca gcaacggctt gatcaattgc
    36601 tgcattgatg gtttcttctg gcatcgcaaa ctgttccggg atcgggttcg ctaccaccag
    36661 gccgccgttc aaaccggttt gccacttcac tgccatcgcg cgagcaattt cgcgggcact
    36721 gtcaagacga atgctgactt caaatgggct ggttcggcag aaaaacgcag gcagcgcttt
    36781 tgtctgataa ccaattaacg gtacaccgaa ggtttctaaa tattctgttg ttaatcctaa
    36841 atcgagaata gattttgccc ccgcacaaac aacagtgaca ttggtatttg ccagttcctg
    36901 taaatcggca gaaatatcga aggtatgttc cgctccgcga tgcacaccac caatacctcc
    36961 ggttgcaaaa actttaatcc cagccagtgc tgcaataatc atggttgatg caacggtagt
    37021 cgcgccattt ttccctgcag caacgacaaa aggtaaatca cgacgactaa ctttcgtcac
    37081 gttatgtcct tcacggccca gcaattcgat ttcttcttta cttaagccca ctttcatcac
    37141 accgccaata atggcgatag tggctggtac tgcgccctgt ttacgaattg tttcttctac
    37201 ttcaattgcg gtttgggcat tttgtgggaa tggcattccg tgagaaatta tggtcgattc
    37261 cagcgcaaca accggttttt tattttttaa agcatcctgt acttcagcgg atatttgtaa
    37321 taattcaggg gacagagtta attcagacat tctgtgttct ccactaacga tctaacattg
    37381 gcaattgata attcagggtt gttggtatat tcacaggcaa gtgccattga cgaacatccc
    37441 tgcgcaaatc gaacagaatc gataaacggc ataccgtcta cccaacagga ggccagcccc
    37501 gccatcatgg cgtcgcctgc cccagtaaca ttgatgacat tcgttttaat aggggccgac
    37561 cacccacttt caccgttgat atcactgtag taaacgccat cccctcccat gctcagcacc
    37621 aagcggttca ggccgtgttg atgaaaccag gcggcgacct ttgcgacatc ttcacgtccg
    37681 gaaagtgcaa ttccgctcaa cgtttcggct tcaagacggt taggttttaa cgtatggatt
    37741 ttgctcagat gatcgcggat tttgacgcat ttccatgccg ataccggatc aataaataca
    37801 ggcgtttccc cgctattttc cagaacccag accagcacct cttcactaag gttgcaatcc
    37861 gcgacaataa cctttgcacc gcgaataaac tcctgatgct gggcaagaaa ttctgcagaa
    37921 atacagtcac taatactcat atcatttatc gcgacaagca tttctcctgt attatcgagc
    37981 agtgataaat agctggatgt attctcaccc ggaacaatta agcatttatc gacataaaca
    38041 ccggattgat tagtttgcgc cagtaatgat tgcccataaa aatcaccacc tactgcactt
    38101 aaaagccagg catttttccc cagtaacgcc agattatgag cgatattcct tcccacgcca
    38161 cctggggtaa atttaatttt tccgggattc gaatccgcat aattcaatgg atgatgtgaa
    38221 tacccagcca catcaatatt cgccgaacca ataattacta tgtagtcctt ttcgcgcata
    38281 gcacgccccc tggcaataaa ttgccaacga agaaattggg taaaatagcc attacagtca
    38341 ataagactat tagagcacgt gttcagattt aaacacatgt tcataataag catatttaac
    38401 gtaatgtaaa tcgatcagaa aaaacgaaat cggccagata tgaaggcggt cacaaagttt
    38461 gtctatttat gcagcaacaa aagagaaagt tgtgatgtgt aacacacaat atggcaggca
    38521 agaaaaaggg cagagatgtt tctgccctcc agattgttga caacgcgcgc tttgttcatc
    38581 ctggatgtgg cgtaaacgcc ttatccgacc tacaaaagcg caaaaaattc aatatattgc
    38641 agggttcatg taggtctgat aagcatagcg catcaggcaa ttttgcgttt gtcatcatcg
    38701 gggcagagat gtctcatccc cgataatgaa gataaattat gcgttttttt ctactatttg
    38761 tgtttccggg cgtttcagga aggcataggc caaacccgcc accagcgtac cggcaataat
    38821 tgctaccagg taacccaata ccggcgtaat agcgccaggg atcagcagaa caaacagacc
    38881 accgtgtggt gccatcagtt tcgcaccaat cgccattgag attgcgccag tcagcgcccc
    38941 accaacgata cagcacggta gcacacgcat cggatcacga gcagcaaacg gaattgcacc
    39001 ttccgaaata aagcacagac caagtaccag agcggctttg ccaccttcct gctgcgcttt
    39061 gtcgaatttg cgacgcgcca ccattgttgc cagacccatt gccagcggtg gcaccatacc
    39121 tgccgccata atcgccgcca tcgggccata ggtttgagta ctcagcagac ccacaccgaa
    39181 tgcgtacgct gctttgttta ccggaccgcc catgtcagta cacatcatgc caccgaggat
    39241 cgcccccagc agaaccgcat tcgcagtccc catggtctgc agccagtgag tcaacccatc
    39301 gagaatgcca gcaaccggtt taccgattag gtagatcatc gccagaccga ccaccagact
    39361 ggaaattagc ggaatgatca ggatcggttt cagcgcctcc atactctgtg gcagtttcaa
    39421 ttgcgtactg attaacttcg caatgtaacc agccaggaag ccagcaatta taccgccaat
    39481 aaagccagaa ccggtgctga ccgccagcat accgccaatc agacccggtg tgaggcccgg
    39541 acgatcggca atggagaagg caatataacc tgccagtacc ggcaccatca gcgcaaaggc
    39601 tgaaccacca ccaatctgca tcagcgccgc tgccaacgta cccggctctt taaacgcttc
    39661 gataccaaaa gcaaaagaaa gcgcgataca cagaccacct gcaacaacca tcggcagcat
    39721 gtaagagacg cccgtcagca agtgacggta agcgcctgca ctctctttct taccttcagt
    39781 ggtcgctgtt tgagctttgc ccgctggttc atacggcgtt gcttcagcaa ccgctttatc
    39841 cagttcctgc gcagttttct tcagcgccag accggtagag gtgcgataca tcggtttacc
    39901 agcaaatttc gccagatcca cttcgatatc tgccgccaca atcaccagat ccgctgctgc
    39961 gacttcttca ggggtgattg cattgcccgc gccaacagaa ccacgggttt caactttcac
    40021 ccaccagcca cgttttttcg cttcggtttc aatggcttca gccgccataa aggtgtgtgc
    40081 tacgccagtc gggcaagcag tcaccgcaac tacgcgtttc ggaccgctgg cagcaaccgg
    40141 tgctgtcgca gcgaccggcg cagtgtaagg tttcgcatgg cctttggctt cactcaggaa
    40201 cagctcaggg tgcgcaactg cccgggaaat atcacccagc cagacatttt taccgttcag
    40261 cgcgctgtca ttcgggatgg aatcaccgag aacaatcgcc atttctgcgt cgttcggatt
    40321 gtcgatgatt tccagttttg cttttcgcgc cgccgcgccc agcagggtct tcgccatata
    40381 ggcgcgtgcc tgaccgagat tagcgtcaat aatcagcagc gttttcatta tgcctctcct
    40441 gctgtcagtt aaaaggttgt aagtcgacgc gcgccatcat tgcggccaac tgcggacgat
    40501 cggtaatacc cacattgctt tgacttaccg ccagggctgc aacagctgtc gccagacgca
    40561 gtgtgtgttc actggattca cgcatcagca agccataaat caggccacca accatagaat
    40621 cccctgcgcc aacggtgctt acgacatcga ctgacggtgg tttggcgatc cattcgccgg
    40681 aggcattaac ccaaagcgcg ccttcggcac ccagtgaaat aacaacatgc gcaatacctt
    40741 gttcacgtag cgcatgtgca gcttcaatca catctttcat ttcaggcagt ttacggcctg
    40801 cccagatttc caactcgcgg cggttaggtt tcaccagcca cggtgccgct ttcaaacctg
    40861 ctactaacgc ttcacggcta ctatcaaaga taatgcaagg acactgacta cgcaggcgag
    40921 tcatccagtc ggtgaacgct tccgggctga cgcctgacgg taagcttccg ctgacacaga
    40981 ccatatcgaa ctgaccgagc cagctcagag aatcagtcac aaagcgttcc cagtcggcga
    41041 gggtgacttc aaaacccgag aagttgaagt cggtcacttc gccgtctttt tccgtcagct
    41101 taacgttaat tcgggtgcgc ccctgtacaa cctggaaacg gttggcaatg cccagctcgc
    41161 tgaacagttg ctgaaaacca tcctgattgt ctttacccag gaagccgcca acggtgacat
    41221 caattcccag gtcttttaat actttggcca cgttgatgcc tttacccgcc gcatgcagac
    41281 cggtggtttt caccaggttc acttcgccgc gttcaatttc cgggcagaaa ccaacaaggt
    41341 cataagccgg attaagggtg atcgtagcaa cacgtctgct cattatgcgc cctccccaag
    41401 accagcagcg atagcgtcgc caatggcttt cagcgcctgt tcagcatctg caccctgggc
    41461 ggtaaagcgt aggcgatgac ctttcttaac gccaagtgcc acaactttca tcagactacg
    41521 tccgtttgcc ggtttgccgg tgccatcaag gtttgtcacg gtaatatcac tgttaaattg
    41581 tttaatggta ttgaccagca tggtacctgg acgagcatgc aggccgtgtt cattgcgcac
    41641 cacaaactcc gcgcttaaca cgtcgtcggt cggcgcatca tcgctggtca gcagcgccag
    41701 caacgttgcc gcatccgctt tcagcaagcg gtcagcttta ttgtcgagca ataaatcagc
    41761 gagacgctta agaaccgcga tgggctgatc gtcattcatc gccacactca ccagcatggc
    41821 tgccgtttcg ccgtccacat caaaagcatt tgccgcacgg cttaccgcaa tcgcgctacg
    41881 cagattgcct tcggcgctat cgctcagcca gataccctgt ccgagattca gcggttgttc
    41941 attgatggct ttggtgacga aagtggcgtc aactgccccc acctctttca gacgcgcagc
    42001 gttcagcgcc tgaagagtca gcagatcgct ggcgacgata tccagcgtca gcatttcgtt
    42061 gtcgagcttc agctgctcac tctgcttttc gcccatcagt aatgcgcgaa gttcttctgc
    42121 tgttgttgct gacttcagtt gttcagcaac ggaatcatcg ctcagtacgt gggtcagctg
    42181 gcgtagcagg cccagatgtt catccgagct ggcagcaata ccgattgcca cgtacgctac
    42241 ctgaccgtca ccccaggtga cgccttccgg gaactgaaat acctgaacgc cggttttcag
    42301 cacctgatcg cgggtgtcgg tagtgccgtg tggaatagca ataccattgc cgaggaacgt
    42361 tgaggtttgc tgttcgcgcg ccagcatgcc attgacgtag ccttctgcta cattaccggc
    42421 ctgcaccagc gccgcagcga cctggcgaat cgcctcttct ttgtctccgg ccttttcgcc
    42481 cggatggatg tcctgtacgg ataactggaa catagttctc ctctcttgct gaattgaaac
    42541 gattcagcct ctatgagaaa aaaagcgcca acctggctta gggttaaaga caagatcgcg
    42601 ctgaaacgtt tcaagaaagc ataatacttc tgtttcagca cgcaaggaaa gccgtaaagt
    42661 tatgagcaaa agtttgatgt gctgcacaat tttttgtcat ttttcctaat tgctgatggg
    42721 aaaatccgtt atcagcaatt tcatttcagc agcgtcagca gccaatcagc aacagtgaaa
    42781 tggcattttg attattcgtg gcgcttttga tttcgctgtc tgtttatttt ctgacaagca
    42841 gcgtaaactc cgcgtcttcc tcttccagtg atcgacctgc atgcataact cccccgcagt
    42901 ctccagcgcg aaatcgtttg acctgacctc gacggcgttt ttaatcgttg cctttctcac
    42961 cggtattgcg ggtgctctgc aaaccccgac actcagtatt ttccttaccg atgaagtaca
    43021 tgcccgtccg gcgatggtgg gattcttctt taccggcagc gctgtcattg ggattctggt
    43081 aagtcagttt ctcgctgggc gctctgataa gcgcggcgat cgcaaatcgc tgattgtctt
    43141 ttgctgcctg ttaggcgtgc tggcctgcac cctttttgcc tggaatcgca actactttgt
    43201 tttgctattc gttggcgtct ttcttagcag ctttggctcg accgctaacc cgcaaatgtt
    43261 tgcccttgcc cgtgaacatg ccgacaaaac cggacgtgag gcggtgatgt tcagctcttt
    43321 tttacgcgct caggtttcac tggcatgggt cattggccca ccgctggctt atgcgttagc
    43381 gatgggtttc agctttacgg taatgtatct gagcgcagcg gtagcgttta ttgtttgcgg
    43441 cgtgatggtg tggctgtttt taccgtcgat gcaaaaagag cttccgctgg cgaccggcac
    43501 ggttgaagcg ccgcgccgta accgtcgcga tacgctactg ctgtttgtca tttgtacatt
    43561 gatgtggggc tcgaacagcc tgtacatcat caacatgccg ctatttatta tcaacgaact
    43621 gcatcttccc gagaaactgg ccgatgtgat gatggggacc gccgccgggc tggaaatccc
    43681 gacgatgttg attgccggat atttcgccaa acgtctgggt aagcgtttct taatgcgcgt
    43741 tgctgccgtg ggtggcgtct gtttttacgc aggaatgctg atggcgcatt cacctgtcat
    43801 tctgttgggc ttgcagctgc taaatgctat ttttattggc attctgggcg gcatcgggat
    43861 gctctatttt caggatctga tgcccggtca ggcgggttca gccaccacgc tctataccaa
    43921 cacttcgcgc gtgggctgga tcatcgcagg atcagtggcg ggcatcgtcg ccgaaatctg
    43981 gaattatcac gctgtgttct ggtttgcgat ggtgatgatt atcgccactc tgttttgctt
    44041 actgcggatt aaagatgttt aaggcgcggt cagcatttcc agatcgataa gccaggtcat
    44101 ggcctgctga cgcgagttac cgcacatttc agcgctggct tgtaagcctg cacagacttt
    44161 cggtcgcagt ggcgaggtga aaattttaca gcgttgctgt tcatcaagct gaatgcaggg
    44221 cgtattggcg ggcttgccat ctggcatacc gggaataggg ctggaaattg aaggggcagt
    44281 gcaacacgcc ccgcaacccg gacggcattc catacgttgt tctcttaagg ttaaccaccg
    44341 gattgatgag cgcgcacagt accacctttt ttgcaccagc aaaagtgcga ataccacttg
    44401 cccgaaaggc ccgtcgcgag tactttgtcg cgatattttt gacattttcg actacaggaa
    44461 tttttcgatg ccaagagcga acgaaattaa aaaaggtatg gtactgaatt acaacggcaa
    44521 actgctgctg gttaaggata ttgatattca gtcgcccact gcccgcggtg ccgctacgct
    44581 gtacaaaatg cgtttttctg atgtccgtac cgggctgaaa gtagaagagc gtttcaaagg
    44641 tgatgatatc gttgacaccg tgacgctgac ccgccgttac gttgacttct cctatgtcga
    44701 tggcaacgaa tatgtcttta tggataaaga agactatacc ccgtatacct tcaccaaaga
    44761 tcagattgaa gaagagttgc tgtttatgcc agagggcggc atgccggaca tgcaggtgct
    44821 gacctgggac ggtcaactgc tggcgcttga gctgccgcag accgttgatc tggaaatcgt
    44881 tgaaaccgca ccaggcatca aaggggcatc tgccagcgcc cgtaacaaac cggcgacatt
    44941 gagcactggt ctggtgattc aggtaccaga atacttaagc ccgggcgaaa aaattcgtat
    45001 ccatatcgaa gaacgccgtt atatggggcg tgctgactaa cttcagccgc atgcagaaaa
    45061 gggatagctc aggctgtccc ttttttaatt tattatacca atcttctatt ttgcgcttcc
    45121 tgaacacctt acgccctcaa ttttcactcg ttgattgatc gccctcacac ttcatcgcat
    45181 taacaatcca gaccaatttc aattgctgtc atataacttt acactgtcat tgttaattta
    45241 tcgttactaa gacgtgactc ctatgaagac aattgcctcc gttacgctcc cgcatcatgt
    45301 acacgctcca cgctacgatc gccagcagtt gcaatcacgt atcgttcatt ttggctttgg
    45361 cgcctttcac cgcgctcatc aggcgttact gaccgatcgt gtgctgaatg cccagggcgg
    45421 cgactggggg atctgtgaaa tcagcttgtt cagcggtgat caactgatga gccagctccg
    45481 cgcacagaac catttatata ccgtgctgga gaaaggtgcg gacggcaatc aggtgataat
    45541 tgtcggtgcc gttcacgaat gccttaatgc aaaactggat tccttagcgg caattattga
    45601 gaaattttgc gagccacagg tggcaattgt ttccctgacg attaccgaaa aaggctattg
    45661 tattgacccg gccaccggtg cactcgacac cagtaatccg cggattattc acgatctaca
    45721 aacccctgaa gaacctcact ccgcaccggg tattctcgtc gaagcactga aacgccgccg
    45781 tgagcgcggc cttacaccgt ttaccgtgct ctcctgcgac aatattcccg acaatggcca
    45841 tgtggtgaaa aacgcggtgc tgggaatggc agaaaaatgt tcgccagaac tcgccgggtg
    45901 gataaaaaaa cacgtcagtt ttccgggaac catggtcgac cgcattgttc cggctgcaac
    45961 cgacgaatca ctggcagaaa tcagccagca tctgggggta aatgatccct gcgcgattag
    46021 ctgcgaaccg tttatccagt gggtggttga agataacttc atcgctgggc gtcctgcctg
    46081 ggaagtcgca ggtgtacaaa tggtgaatga tgtcctgcca tgggaagaga tgaaactgcg
    46141 tatgcttaat ggcagccact cttttctcgc ttatctgggt tacctctcag gattcgtcca
    46201 tatcagtgat tgcatgcagg atcgcgtatt tcgccatgct gccagaacat taatgctgga
    46261 tgagcaagcg ccgacactgc aaattaaaga tgtcgattta acacaatatg cggataagtt
    46321 aattgcacgt tttgctaatc cgtcgctgaa acataagacc tggcaaatcg cgatggatgg
    46381 cagccagaaa ttaccgcaac gtatgctggc aggtattcgc atacatctgg ggcgtgaaac
    46441 ggactggtcg ttgctggcat taggcgttgc tggctggatg cgttacgtca gcggcgttga
    46501 tgatgccgga aatgccattg atgttcgcga tccgcttagc gataaaattc gcgaacttgt
    46561 tgcgggcagc agcagtgaac aacgcgtaac cgccctgctt tccctgcgtg aagttttcgg
    46621 tgatgatctg ccagataacc cgcattttgt gcaggccatc gaacaagcct ggcaacaaat
    46681 cgtacaattc ggcgcacatc aggcgctatt aaacaccctc aaaatttaac gatttctgcg
    46741 gttaaagcgg atgaagctca ccttcgtccg ctctcccctt ctcttttctg ccttttttag
    46801 ccaggattaa cgctcagtta acttaccaga gtattgcgga gccgttgtga ccaggaccaa
    46861 cctcatcacc ggttttctcg gcagcgggaa aaccacgtcg attcttcatc tgttagccca
    46921 taaagatccc aacgaaaaat gggcggtact ggttaatgaa tttggggaag tcggaattga
    46981 tggtgctttg ctcgccgata gcggcgcatt gctgaaagag atccccggcg gctgcatgtg
    47041 ctgcgttaat ggtttaccca tgcaggtagg gttgaatacc ttactgcgtc agggaaaacc
    47101 agaccgcttg ttgatagagc cgaccgggct gggccatccg aaacagatcc tcgatctgtt
    47161 aaccgcacca gtctatgaac tgtggataga tctgcgcgcc accttgtgca ttctcgatcc
    47221 acgcctgctg ctggacgaaa aaagcgccag caatgaaaac ttccgtgacc agctggctgc
    47281 cgcagacatc attgtcgcca ataaatccga ccgtgcgacg cccgaaagtg agcaagcgct
    47341 acagcgttgg tggcagcaaa atggtggcga tcgacaatta attcacagtg agcatgggaa
    47401 agttgacggt catcttctgg atttgccgcg tcgcaattta gccgagttgc ccgccagcgc
    47461 cgcgcattct catcagcatg tcgtgaaaaa agggttagca gcgttaagcc tgccagagca
    47521 tcaacgctgg cgtcgcagtc tgaacagcgg gcaaggatat caggcctgcg gctggatatt
    47581 cgacgctgat acggtattcg acaccattgg cattctggaa tgggcgcgac ttgcaccggt
    47641 ggaacgcgtc aaaggcgtgc tgcgtattcc cgaagggctg gtacgaatca accgtcaggg
    47701 cgatgacctg cacattgaaa cgcaaaacgt tgcgccaccg gacagccgta ttgagctgat
    47761 ttccagcagc gaagctgact ggaatgcttt gcagagcgcg ctgttgaagc ttcgtttagc
    47821 gactaccgcg taaggttgcc tgcgtttttc agtaagataa ttagagaaaa tatgattaaa
    47881 aatttgccgc aaatagtgtt gttgaatatt gtcggcctcg cgctgtttct ttcctggtat
    47941 attcccgtta atcatggatt ctggttgccg attgatgcgg atatttttta tttctttaat
    48001 cagaaactgg tcgaaagtaa ggcctttttg tggctggttg cattgaccaa caatcgcgct
    48061 ttcgacggtt gttctctgct ggcgatggga atgttgatgc tgagtttctg gctgaaagaa
    48121 aacgcccctg gcagacgacg tatcgtgatt atgggtctgg tcatgctatt aactgcagtg
    48181 gtattaaacc agctgggtca ggcattaatt cctgtaaaac gggccagccc aacattgact
    48241 tttaccgata ttaaccgcgt cagcgaactg ctctctgttc ccacgaaaga tgcctcacga
    48301 gatagctttc ccggcgatca cggcatgatg ctgcttattt tttcggcatt catgtggcgt
    48361 tatttcggca aagttgcagg ccttatcgcc cttattattt ttgtggtttt tgcatttccc
    48421 agagtaatga ttggcgcaca ctggtttact gacatcattg tcggttcgat gaccgtgata
    48481 ttgatcggtt tgccctgggt gttgctgacg ccattaagtg atcgattaat cacctttttt
    48541 gacaaatcac taccgggaaa aaacaaacat ttccaaaaca aataactcac agtaattaac
    48601 atcatcaggc ttatttttat agtgaggata atcctgatga tgcgcaccgt gctttcatcc
    48661 atcgcgcgca aaaatcattc tctaagtaaa tgaatggatt gcatgcgttt cactcaatcg
    48721 tactttaatt gaccaatccc gcttattaac tttctgtatc actttttctt ataaaaaatc
    48781 atgtaaaacc gctcgccaag accgcaccaa tcgggtaatc tagaactcgt tttgcctcgg
    48841 cggtagatta tcctcacagc atataatttt gtgcgttagt ccacagattt ggccttaagg
    48901 aattgtttca acatgcccag gtaattagtc tcgtgtcgct tggcattttt ttataacgat
    48961 atttgtcgtt aaggacttca agggaaaaca aacaacatgg tcaaatctca accgattttg
    49021 agatatatct tgcgcgggat ccccgcgatt gcagtagcgg ttctgctttc tgcatgtagt
    49081 gcaaataaca ccgcaaagaa tatgcatcct gagacacgtg cagtgggtag tgaaacatca
    49141 tcactgcaag cttctcagga tgaatttgaa aacctggttc gtaatgtcga cgtaaaatcg
    49201 cgaattatgg atcagtatgc tgactggaaa ggcgtacgtt atcgtctggg cggcagcact
    49261 aaaaaaggta tcgattgttc tggttttgta caacgtacat tccgtgagca atttggctta
    49321 gaacttccgc gttcgactta cgaacagcag gaaatgggta aatctgtttc ccgcagtaat
    49381 ttgcgtacgg gtgatttagt tctgttccgt gccggttcaa cgggacgcca tgtcggtatt
    49441 tatatcggca acaatcagtt tgtccatgct tccaccagca gtggtgttat tatttccagc
    49501 atgaatgaac cgtactggaa gaagcgttac aacgaagcac gccgggttct cagccgcagc
    49561 taataaaccg tttggatgca atcccttggc tatcctgacg agttaactga aagcactgct
    49621 taggcagtgc ttttttgttt tcattcatca gagaaaatga tgtttccgcg tcttgatcca
    49681 ggctatagtc cggtcattgt tatcttttaa atgttgtcgt aatttcagga aattaacgga
    49741 atcatgttca tacgcgctcc caattctgga cgtaagctcc tgcttacctg cattgtagca
    49801 ggcgtgatga ttgcgatact ggttagctgc cttcagtttt tagtggcctg gcataagcac
    49861 gaagtcaaat acgacacact gattatcgac gtacaaaagt atctcgatac ctattttgcc
    49921 gacctgaaat ccactactga ccggcttcag ccgctgacct tagatacctg ccagcaggct
    49981 aaccccgaac tgaccgctcg cgcggcgttt agcatgaatg tccgtacgtt tgtgctggtg
    50041 aaagataaaa aaacattctg ttcatctgcg actggtgaga tggacattcc actcaatgaa
    50101 ttgattccgg cgctcaacat taataaaaac gtcgatatgg cgatcttacc cggcacgccg
    50161 atggtgccga acaaacccgc aatcgtcatc tggtatcgca accctttgct gaaaaatagc
    50221 ggtgtgtttg ccgctctgaa tctcaacctg acgccttctc tcttttatag ttcacggcag
    50281 gaagattacg atggcgtcgc cctcattatt ggcaatactg cgctatctac cttttcttca
    50341 cgtttgatga acgttaatga attaaccgac atgccggtcc gtgaaactaa aattgcgggc
    50401 attcctctga ccgttcggct ttatgcggat gactggacat ggaacgatgt gtggtacgca
    50461 tttttactgg gcggcatgag tggaactgtc gttggcctgc tctgctatta cctgatgagc
    50521 gtacgtatgc gccccggcag agaaatcatg accgccatca agcgcgaaca attttacgtg
    50581 gcgtatcaac cggtggtgga tacacaagct ttgcgagtaa cgggcctgga agtactgcta
    50641 cgctggcggc atcctgtcgc gggagaaatt cccccggatg ccttcattaa ctttgccgaa
    50701 tcgcaaaaga tgattgtgcc gctgactcag cacctgtttg agttaattgc ccgcgatgcc
    50761 gcagaattag aaaaagtgct gccagtaggc gtcaaatttg gtattaacat tgcgccggac
    50821 catctgcaca gcgaaagctt taaagcagat atccagaaac tgctcacttc cctgcccgca
    50881 caccatttcc agattgtgct ggaaattacc gagcgcgata tgctgaaaga gcaagaagcc
    50941 acacaactct tcgcctggct gcactcggtc ggcgtagaaa ttgctattga tgacttcggc
    51001 accgggcaca gcgcgcttat ctatcttgag cgttttacgc tcgattatct gaaaattgac
    51061 cgtggattta tcaacgccat cggtacggaa acgatcactt cacccgtact tgacgcggtg
    51121 ctgacgctgg cgaaacgcct caatatgctg acggttgctg aaggggtcga aacgccggaa
    51181 caggcgcgat ggctaagcga acgcggcgtt aatttcatgc aaggctactg gattagtcgc
    51241 cctttaccgc tggacgattt tgtccgctgg ctgaagaaac cgtatacgcc gcagtggtaa
    51301 ggtgtgctta cgtcccttat tattcatagt gaaagcatgc cggattgcgg ctaatgatga
    51361 gtaaaaggaa atccattgca gatgattgtg cgcatactgc tgctgtttat cgctctgttc
    51421 acctttggtg tgcaggcgca ggctatcaag gaaagctatg cctttgccgt gctgggcgaa
    51481 ccccggtacg cgtttaattt caaccatttt gattatgtga accccgccgc gccaaaaggt
    51541 gggcagataa cgttgtcagc cctcggcacc ttcgataatt tcaaccgcta tgcactgcgc
    51601 ggcaacccgg gcgcacgcac cgagcagctg tacgacacgc tatttacgac ttccgatgac
    51661 gaaccaggca gttattaccc gctgattgct gaaagcgcac gctatgctga cgattattcc
    51721 tgggtggagg tcgctattaa tccgcgcgcc cgttttcatg atggttcgcc cattactgcc
    51781 cgcgatgtag agtttacttt tcaaaaattt atgaccgaag gcgtgccgca atttcgtctg
    51841 gtctacaaag gcaccaccgt caaagccatt gcaccgttaa ccgtgcgcat tgagttagct
    51901 aaacccagca aagaagatat gctgagtctg ttttcgctgc cgatatttcc agaaaagtac
    51961 tggaaggatc acaaacttag cgacccgctc gccacgcctc cgcttgccag tggtccgtac
    52021 cgcgttacgt cctggaaaat ggggcaaaat attgtctatt cccgcgtgaa agattactgg
    52081 gcagcaaact taccggtaaa ccgtggacgc tggaatttcg acaccattcg ctacgattat
    52141 tacctcgatg ataatgtcgc ctttgaagcg tttaaagcag gtgcctttga tttgcgtatg
    52201 gaaaacgacg ctaaaaactg ggccacgcgt tataccggta aaaatttcga taaaaaatac
    52261 atcatcaaag atgagcaaaa gaacgaatca gcccaggata cgcgctggct ggcgtttaat
    52321 atccaacgtc cggtattcag cgatcgccgg gtccgggaag caatcactct cgcctttgac
    52381 tttgaatgga tgaacaaggc gttgttttac aacgcctgga gtcgcacgaa cagttatttt
    52441 cagaataccg aatacgcggc cagaaattac cccgacgccg cggagctggt gcttctggca
    52501 ccaatgaaaa aagatctacc gccagaagtc ttcacacaaa tctaccagcc gccggtatct
    52561 aaaggcgatg gctacgatcg tgacaacctg ttaaaagccg acaaacttct caacgaaacg
    52621 ggctgggtgc tgaagggtca gcaacgcgtt aatgccacaa cgggtcagcc gctcagcttt
    52681 gaattattgc ttcccgcaag cagcaatagt cagtgggtat tgccgttcca gcacagcctg
    52741 caacggctgg gtatcaatat ggacattcgc aaggtggata actcgcaaat cactaaccgc
    52801 atgcgcagtc gcgactatga catgatgccg cgcctatggc gggcgatgcc gtggcccagt
    52861 tccgatttac agatttcctg gtcatcggaa tatatcaatt ccacttataa tgcccccggc
    52921 gtgcaaagcc cggttatcga ctcgctgatc aaccaaatta ttgccgcgca gggaaataaa
    52981 gaaaaattac tgccgttggg gcgagcactg gatcgcgtat taacgtggaa ttattacatg
    53041 ctgccaatgt ggtacatggc ggaagaccgt ctcgcctggt gggataaatt ctcccaaccc
    53101 gctgtacgcc ctgtttacag cctgggtatc gatacctggt ggtatgacgt taataaagcg
    53161 gccaaactgc cgtcagccag acaacaggga gagtagatgg gcgcttacct gattcgccgt
    53221 ctgttgctgg tgatcccaac gttatgggct attatcacta tcaacttttt catcgtgcaa
    53281 attgcgcctg gcggtccggt cgatcaggcc atcgccgcca ttgagtttgg taatgccgga
    53341 gtattacccg gcgcaggcgg tgaaggtgtt cgtgccagcc atgcgcaaac gggtgtcggc
    53401 aatatcagcg acagtaatta ccgtggcgga cgcggattag acccagaagt gatcgctgag
    53461 atcactcatc gctacggttt cgataagccg atccacgaac gttacttcaa aatgctctgg
    53521 gactacatcc gctttgattt tggtgatagc ctgtttcgca gcgcctcggt gctgacgctg
    53581 attaaagaca gtctgccggt ttccatcacc ctcggattgt ggagcacgct gattatctat
    53641 ctggtgtcga ttccgttagg cattcgcaaa gctgtttata atgggagtcg ctttgacgtc
    53701 tggagtagcg catttatcat catcggctac gccattccgg cctttttgtt tgccatcctg
    53761 ctgattgtct tcttcgcggg cggcagctat ttcgacctgt tccctctacg cggcctggtt
    53821 tccgctaact ttgattcgct gccgtggtat cagaaaatca ccgattatct gtggcatatc
    53881 acgctaccgg tgctggcgac agtgattggt ggctttgcgg cgctgaccat gctgaccaaa
    53941 aactcattcc ttgatgaagt gcgcaagcaa tacgtggtga ccgcgcgtgc gaaaggggta
    54001 agtgagaaaa acattctctg gaaacatgtg ttccgcaacg ccatgctgct ggtgattgcc
    54061 ggttttccgg cgacgtttat cagcatgttt tttaccggct cgctgctgat tgaggtgatg
    54121 ttttcactca atggtcttgg cttactgggc tacgaagcga ccgtctcgcg cgattatcct
    54181 gtaatgtttg gtaccttgta tattttcacc ctgattggcc tgctgctgaa tattgtcagt
    54241 gatatcagct atacgctggt tgatccgcgt atagattttg agggacgtta atgtcgcgac
    54301 tcagccccgt caatcaggcc cgttgggcgc gttttcgtca taaccgtcgc ggctactggt
    54361 cgttatggat tttcctcgtt ttgtttggtt tgagtttgtg ttctgaactt atcgctaacg
    54421 ataaaccgtt gctggtgcgt tatgacggca gttggtattt cccgttgttg aaaaactaca
    54481 gcgaaagcga ttttggcggc ccgctggcaa gtcaggctga ttatcaggac ccgtggctga
    54541 aacaacggct ggaaaataac ggctgggtac tgtgggcacc gattcgcttt ggtgctacca
    54601 gtatcaactt tgctaccgat aagcccttcc cttctccacc ctcccggcaa aactggctgg
    54661 gaacggatgc caacggcggc gatgtgctgg cacgcattct ctatggcacg cggatctcgg
    54721 ttctgtttgg cctgatgctg actctctgtt ccagcgtgat gggcgtgctg gcgggggcgc
    54781 tacaaggcta ttacggcggt aaagtcgatc tctggggaca acgctttatt gaagtatggt
    54841 cggggatgcc gacgctgttt ttgattattt tactttccag cgtcgtacag cctaacttct
    54901 ggtggctgct ggcaattact gtcttgtttg gctggatgag tctggtcggc gtggtgcggg
    54961 cggagttttt acgtactcgt aatttcgact acattcgtgc ggcacaggcg cttggcgtca
    55021 gcgatcgcag tatcatcctg cgtcatatgt tgcctaatgc catggtcgcg accctcacct
    55081 ttttaccgtt tattttatgt agttcgataa ccaccctgac ctcgctcgat ttcctcggct
    55141 tcggtctacc gctcggttca ccgtcactcg gtgaactgct gttacaaggg aaaaataacc
    55201 ttcaggcccc gtggcttggg atcaccgcct tcttgtcggt ggcgatatta ttgtctttgc
    55261 tgatctttat tggtgaagcc gtccgcgacg catttgatcc taataaggcg gtgtagcatg
    55321 acgcaaactc tgttagcgat tgaaaatttg tcggtgggtt ttcgccatca gcaaaccgta
    55381 cgtacagtag tcaatgatgt ttcactacag attgaggctg gcgaaacgct ggcgctggtg
    55441 ggtgagtcag gttcaggcaa aagcgttacc gcgctgtcaa ttttacgcct gctcccttcc
    55501 ccgccggttg aatatctctc cggcgatatt cgttttcatg gcgaatcgct gcttcatgcc
    55561 agcgatcaaa cgttgcgcgg tgtacgcggt aataagatcg ccatgatttt tcaggaaccg
    55621 atggtgtcgt taaatccatt gcataccctg gaaaaacagc tttatgaagt gctttcactc
    55681 caccgcggga tgcgtcggga agcggctcgt ggcgaaattc ttaactgcct tgatcgcgtc
    55741 ggtatccgcc aggcggcaaa acggctgaca gattatccgc atcagctctc cggcggcgaa
    55801 cgccagcggg tgatgattgc gatggcgctg ttaacgcgac cggaattatt aattgccgat
    55861 gaaccgacca ccgcactgga cgtctctgtc caggcgcaga ttttacagct gttgcgcgaa
    55921 ctgcaaggcg agctgaatat gggcatgctg tttattactc ataacctcag cattgtcaga
    55981 aaactggccc accgcgtggc ggtaatgcaa aacggtcgct gtgtcgagca aaataacgcc
    56041 gctacgctat ttgcctcacc cactcatcct tacacacaaa agctactcaa cagtgaaccg
    56101 tcaggcgatc cggtaccgtt gccagaacct gcctcaacgt tgctggatgt tgaacagctt
    56161 caggttgcct tccccattcg caaagggatt ttgaagcgca ttgtggatca taatgtggtg
    56221 gtgaaaaaca tcagttttac gctacgggcg ggtgaaacac tgggtttagt gggcgagtct
    56281 ggttccggga aaagcacgac gggactggcg ctgctgcgac tgattacctc tcagggcagc
    56341 atcgtctttg acggtcagcc actgcaagat ttaaatcgcc gccagctgtt acctactcgt
    56401 catcgtattc aggtggtatt tcaggatcca aactcctcgc tcaacccacg actcaacgtt
    56461 ttgcagatta ttgaggaagg cttacgggtt caccagccga cgctttctgc cgcacaacgc
    56521 gaacaacaag tgatagccgt gatgcatgaa gtgggattag atcctgaaac acgccaccgt
    56581 tatccggcgg agttctctgg tggtcagcga caacgtattg cgattgccag ggcattaatt
    56641 cttaagccct cactgatcat acttgatgaa ccgacatcat cactcgacaa aacggtacag
    56701 gcgcaaatat tgacgctatt gaaatcattg caacaaaagc atcaactggc ctatttgttt
    56761 atcagccacg atttgcacgt tgtccgcgcg ttatgtcatc aggttatcgt actgcgacaa
    56821 ggggaagtag tggaacaagg accgtgcgcg cgcgtgtttg ccgcaccgca gcaggagtat
    56881 acgcgtcagc tactggcgtt gagctgacgc ttaaaaagga ttgtagtttg aaaagggttc
    56941 ggcaattgcc acaccaaaat ttttcaatcg gcatgttgca gcaaactcgt cctggcgatt
    57001 tacaaacagg cacggctcac cttcacactc cagcaccgaa catggaactt cgatgtcgct
    57061 taacgcctgg cttagtttat acatcaccga ccatgcatta tcaccatccg ggctaagcaa
    57121 tttaagcgcc accaggctgt tgtcaccgca cggtccattt tgcggaatcg gttcaacctt
    57181 cgaacctgcg aaaccagcag accagcgaaa gttctgcggt agacgatgga cgattgagag
    57241 ttgtaatgaa gtcacttatt ttccccggaa gcacatttac ttcacaattt atttacatca
    57301 attttaacac atcatcaaca atccgtcttt atacagatcg taaagattga tgcctcgcct
    57361 gcatggcctc tatggctctg atttaagtaa tatctgcggg cgtggtcacg tttgcggggc
    57421 tatatgtacc cgtttctgcg atctaactca acctttttaa ctacaatgat gtgacttttt
    57481 acataaattg attttacata aaataaacat atatcggggg aatgatagat ttgtggttga
    57541 catgcatcaa caaaggaagc cttttagctt cctcgttgtg caatagatca ccgttttttc
    57601 ggtcgactgg cgtacagaca gaagagaatg gagctggttg cgcagaatgc aattgaccaa
    57661 atcatcggcc atgcagagtt aaaggtcgca agagaaagca atgcgccaac aattgcccct
    57721 atgccaaaac ggaaggttcc tgccagcgaa gatgccgttc ccgccatatg gggaaactca
    57781 tcaagaatga ccgccatcgc attggatgac accatagaca cgcagcccac aaacgccgca
    57841 acgccaacca ccagcgacca gaatcccagc cccagcagcg cactgatgac catccacgct
    57901 gccataataa attgtatcca caaccccgag cggaacatat ttaacgcgcc aatgcggcgg
    57961 acgaagcggc tgttaaagat ggtcatcacg aacagaaaaa caatgtttag cgcaaagtaa
    58021 taaccaaagt tttccggtgc aacgtggttg atttcaatgt agacaaacgg tccggcgctc
    58081 aggaacgaga acattccggc aaagctgaaa ccgctggcca gcatataact caggacacgc
    58141 ttatgacgga acagcgccgc aaagttacca atagtggtac gaatgtgaaa aggctggcga
    58201 ggatccagaa gatgtaatgc cagctcagcc acaccagcac ccagccgcca actatcggtg
    58261 ccatcagcgg tgcaatggtc gtcaccagca tgacaaacga catcatccgc gagaactctt
    58321 ctttcgggta aatatcacgc atcaaagcgt tgataaccac gctggccgca gccgcagcca
    58381 gcccgtggaa gaaacgcatc acaatcagct gatcgatggt ttgtgccaac gcacacgcca
    58441 ccgcggcggc ggcaaacacc agcgtaccgc caagaaccac cggcttacgc ccgaagctgt
    58501 ctgccatcgg cccgtagatt aactgcccca acgcaaagcc cagaatataa gtactcaggg
    58561 tcatctgcgt actgcccgcc ggtacgccaa actgcgctga aattaccggt agcgcgggca
    58621 gatacatatc aatcgacagc ggcatcaaca tggccagcag gccaaggata aaaacaatag
    58681 caaacgacga atgctgtcgg gtggtcacaa cgggctcctg aaagtcattg aaaagttaga
    58741 cgacgctggc aatttcttct tcagttaacg gacgatattc accgggggct aaatcagcat
    58801 ccagcgtaat gccgccaata cgttcacgat gcagctcaac cacatggtta cccacggcgg
    58861 cgaacatgcg tttcacctga tgataacgcc cttcgctgat ggtcagacga acctgcgttg
    58921 gggtaatcac ttccagcacc gccggcttag tgagatcttt ttcgttatgc agctgcacgc
    58981 ctttagcaaa ttgttctgcc gtatcgtcag ctacgggtga taccagcgtc accagatagg
    59041 tcttctcgca atgatggcgc ggagaagtaa tgcggtgcga ccactgacca tcatctgtca
    59101 ttagtaccag accggtggta tcgatatcca gccgccctgc ggcgtgcagt ttccacgcta
    59161 ccggttcatc tagaaaatag agcaccgttg ggtgatcagg gtcgtccgtg gagcaaacat
    59221 aaccctgagg cttattgagc atgaagtaac gtggaccgtg ttgctgcgcc agcgggttgc
    59281 catcgtaagc gacatcatgt tcaggaagca gtttgaacgc tgcattacgg acgatttcgc
    59341 catcgacggt gacacgattg ccgcggattt cacgcccggc aatagcacgg ctaacgccga
    59401 gttgctgtgc gataaattta tcaagtcgca tgtgtgtgat tttgcctgta aaaaacggag
    59461 gtcgggtaat gacccgaata tctgaactgt tgtctgccca gtatagcggt ctaataacat
    59521 ccctcaaggg aaaaagatcc gtggcatact attagcagaa taatctacct acgcgagacc
    59581 atgattttta cacttcgccc atatcagcaa gaagccgtgg atgccacgct caaccatttt
    59641 cgtcgtcata aaacccctgc cgttatcgtg ctgcccaccg gtgcaggtaa aagcctggtg
    59701 atagcggaac tggcacggct ggctcgtggt cgcgtactgg tgctggcaca cgttaaagaa
    59761 ctggtggcgc aaaaccatgc aaagtatcag gcgctggggc tggaagccga tatttttgcc
    59821 gccgggctga agcgtaaaga gagccacggt aaagtggtat ttggcagtgt gcagtcggtc
    59881 gcccgtaata ttgatgcctt tcagggtgaa ttttcgctgt tgattgtcga tgaatgtcac
    59941 cgtattggtg acgatgaaga gagccagtat cagcaaatcc tcactcacct gacaaaagtg
    60001 aatccccact tacgcctgct ggggctgact gccacgcctt ttcgattggg caaaggttgg
    60061 atctaccagt tccattatca cggcatggta cgcagcgatg agaaagccct tttccgtgac
    60121 tgcatttatg agctgcccct gcgttatatg attaaacaca gctatctgac gccgccagaa
    60181 cgactggata tgccagtagt gcaatatgat ttcagccgct tgcaggcaca gagcaacggg
    60241 ctgttcagcg aagccgatct caaccgtgag ctgaaaaaac agcaacgtat taccccgcac
    60301 atcatcagcc agattatgga gttcgccgaa aagcgtaaag gagtgatgat tttcgccgcg
    60361 acggtcgaac acgcaaaaga gattgtggga ttactgcctg ccgaagatgc agcactgatt
    60421 actggcgaca cccccggcgc tgagcgcgat gtgttaattg aaaattttaa agcccagcgt
    60481 tttcgctatc tggtcaacgt cgcggtactg accaccggat ttgacgcccc gcacgtcgat
    60541 cttatcgcca ttctgcgccc taccgaatca gtgagtcttt accaacaaat tgtcgggcgc
    60601 ggtctgcgtc tcgctccggg caagactgat tgtttaattc ttgattatgc gggtaatcct
    60661 cacgatctct acgcgccgga agttggtaca ccgaaaggca aaagtgacaa cgttccggta
    60721 caggttttct gccctgcctg cggttttgcc aacacctttt gggggaaaac gaccgccgac
    60781 gggacattga ttgaacactt tggtcgccgc tgtcagggat ggtttgaaga tgacgacggt
    60841 catcgcgaac agtgtgactt ccgtttccgt tttaaaaatt gcccgcaatg taacgcagaa
    60901 aatgatattg ccgctcgccg ctgccgcgaa tgtgacacgg tactggttga tccggatgat
    60961 atgttaaaag cggcgctacg actgaaagat gcgctggtat tacgctgtag cggcatgtct
    61021 ttgcagcatg ggcacgacga gaaaggcgaa tggttgaaaa tcacctatta cgatgaagac
    61081 ggtgcggatg tgagtgagcg tttccgtctg caaacacctg cccagcgtac cgccttcgag
    61141 cagcttttta tccgcccgca tacgcgcaca ccgggcatcc cgctgcgctg gatcaccgcc
    61201 gccgatatcc tcgcccagca agccttattg cgacccccgg attttgtcgt cgcccgcatg
    61261 aaaggccagt actggcaggt gcgtgaaaaa gtgttcgatt acgaaggtcg ttttcgtcgg
    61321 gcgcacgaat tacgcggtta atggtagttt tgattgatgt ataaggcgat tgagtataga
    61381 atctcacccg cttttgcata cgcaaagcag atcacttacc tgttgctggg tcgcctgtag
    61441 caggattaat ttaagagaga aagaaatgtt tactatcaac gcagaagtac gtaaagagca
    61501 gggtaagggt gcgagccgcc gcctgcgtgc cgctaacaag ttcccggcaa tcatctacgg
    61561 tggcaaagaa gcgccgctgg ctatcgagct ggatcacgac aaagtcatga acatgcaagc
    61621 taaagctgaa ttctacagcg aagttctgac catcgttgtt gacggtaaag aaatcaaagt
    61681 taaagctcag gacgtacagc gtcacccgta caaaccgaag ctgcagcaca tcgacttcgt
    61741 tcgcgcttaa ttgctgaata agttgtaaaa aaccccgctc ccgcggggtt ttttgtatct
    61801 gcagattatg cctgattacg gtattgctat ttcttgcagg ccagataagg cgttcacgcc
    61861 gcatctggca ttcagaacac aaaaccgatt aattgccgcc agacgtccgg cgctgcaatt
    61921 ggtcgcgcaa attcggcggc gtgcctttaa tggtcagggt atcggtcgcc ggctcccaga
    61981 agatgcgttc acctaacagc atcgcatcaa agttaatagt caagccaccg ccgctaccgg
    62041 caaacttcgt caactgacgc agtgtgctcc ggtctgccgg gaagctctct tccagttcgt
    62101 agcctttttc agccgcaaac tccgtgaagc tcacttcgct tacgcctgcc agctctttcg
    62161 acagcgattc cagttcgatc tcttcgcctg cctgcagctg ctcattacag tagctataaa
    62221 cctgctggcg cacgttctgt cgctctgctt tatccagttg tgcttcggca gtgaaatcat
    62281 caaccgcttg caacagccca cggttttgtg ctttcgtgtt caatccttcg ctggccccga
    62341 ggaaatccat aaagaaatcc gccactttgc gccctactcg ccctttcagg aaagtgagat
    62401 agcgggtgga ctctggattg gtttcccatt cggttaaatc gatacgcgca acaatatccg
    62461 catgattgat atcaagataa tgggttgggt tgatgtcgag attttcgtta acacgcatac
    62521 tgctcaggtt gctcagaact gccaccagca gatactccac cgccagataa cggtagtggc
    62581 aaaacagcac gaatccaccg tcagcaaaag gatatttcgc taattcgtca cgcaggcgac
    62641 cagttgccgc gcggctaaat gccagaaagt cctcttcgcc ctgacgctgt aagcgcagcg
    62701 tctgcgccag ttcactctct tcgctaaaca agccgtaagc tttatttttg gcgctataga
    62761 cccgatgcag ttctgccacc atctcaacga cggtttctgt cggttccagc aatgaatcgc
    62821 gcaacaccag ctcaaggttc tgctcatcac gcttgataag ctggtgcaga gcaatctggt
    62881 tgatatccag actcatgata aactctcctt taagaccggg cggtattcaa ccaccgccgg
    62941 ttaagcgacg caataaaaga tggcggtcat tttacagaag gcagcgtaac gagaatgatt
    63001 taacgcataa aaaagcagaa aaaaaaccgt tgctacggta atatgttgcc ctttcatcaa
    63061 ctaactgatt tcgatttatg ccacaaattt cccgctacag tgatgaacag gttgaacaat
    63121 tgctcgcaga gctgctcaac gtactggaaa aacataaggc tccgaccgac ctttccctga
    63181 tggtgttagg caacatggtc actaacctta tcaacaccag cattgccccg gcccaacgcc
    63241 aggcaattgc caactctttt gcccgcgcct tacagtcctc tatcaacgaa gacaaagcgc
    63301 actaagggaa acagataaca ggttatggta actcatcgtc agcgctaccg tgaaaaagtc
    63361 tcccagatgg tcagttgggg gcactggttt gcactgttca atattctgct ttcgctcgtc
    63421 attggcagcc gttacctgtt tatcgccgac tggccgacaa cgcttgctgg tcgcatttat
    63481 tcctacgtaa gcattatcgg ccatttcagc ttcctggtgt tcgccaccta cttgctgatc
    63541 ctcttcccgc tgacctttat cgtcggctcc cagaggctga tgaggttttt gtccgtcatt
    63601 ctggcaacgg cgggaatgac gctattactg atcgatagcg aagtctttac tcgtttccat
    63661 ctccatctta atcccatcgt ctggcagttg gttatcaacc cagacgaaaa tgagatggcg
    63721 cgcgactggc agctgatgtt catcagcgtg ccggttattt tattgcttga actggtgttt
    63781 gcgacgtgga gctggcaaaa gctgcgcagc ctgacgcgtc gtcgacgctt cgcgcgcccg
    63841 ctggccgcat tcttatttat cgcctttatc gcctcgcatg tggtgtatat ctgggccgat
    63901 gccaacttct atcgcccgat caccatgcag cgcgctaacc tgccgctttc gtacccgatg
    63961 acggcgcgac gttttcttga gaagcatggt ctgcttgatg cgcaggagta tcaacgccgt
    64021 cttattgagc aaggtaatcc agacgccgtt tccgttcagt atccgttaag cgaactgcgc
    64081 tatcgcgata tgggcaccgg gcagaatgtg ctgttgatta ctgtcgatgg cctgaactac
    64141 tcacgcttcg agaagcagat gcctgcgctg gcaggttttg ctgagcaaaa tatttcgttc
    64201 acgcgccata tgagctccgg caacactaca gacaacggca tctttggcct gttctatggt
    64261 atctcgccga gctatatgga cggcattctg tcgacccgta cgcctgcggc attaattact
    64321 gcgcttaatc agcaaggcta tcagctgggg ttattctcat cagatggctt taccagcccg
    64381 ctgtatcgcc aggcattgtt gtcagatttc tcgatgccga gcgtacgcac ccaatccgac
    64441 gagcagaccg ccacgcagtg gatcaactgg ctgggccact acgcacaaga agataaccgc
    64501 tggttctcgt gggtttcttt caatggcact aacattgacg acagcaatca gcaggcattt
    64561 gcacggaaat atagccgggc ggcaggcaat gtcgatgacc agatcaaccg cgtgctcaat
    64621 gcactgcgtg attctggcaa actggacaat acggttgtga ttatcactgc cggtcggggt
    64681 attccgctga gcgaagacga agaaaccttt gactggtccc acggtcatct gcaggtgcca
    64741 ttagtgattc actggccagg cacgccggcg cagcgtatta atgcgctgac tgatcatacc
    64801 gatctgatga cgacgctgat gcaacgcctg ctacatgtca gcacacctgc cagcgaatat
    64861 tcgcaaggtc aggatttgtt caaccctcaa cgccgtcatt actgggttac tgcagcggat
    64921 aacgatacgc tggcaattac caccccgaaa aagacgctgg tgctgaacaa taacggtaaa
    64981 taccgcactt acaacttacg tggtgaaaga gtgaaagatg aaaaaccaca gttaagtttg
    65041 ttattgcaag tgctgacaga cgagaagcgt tttatcgcta actgattaat tataaatcag
    65101 ttagcgaaat atcttacttg caatcggtgt ggaaaacggt agtattagca gccacgagtc
    65161 ggcacgtagc gcagcctggt agcgcaccgt catggggtgt cgggggtcgg aggttcaaat
    65221 cctctcgtgc cgaccaaaaa tcccaagaaa aaaccaacct ttacggttgg tttttttata
    65281 tctgcaatta atttgataaa cagaccgtga cacatcacag cctgtttatt ttctgttatc
    65341 agaacgtcca gaccactccc gcctgagtat tccacggcga ttctacccct gcgccattgc
    65401 catagctgac tgacaaatga ccgcttaacg tcggggtaaa cgatgacctt atcccagcct
    65461 gataaacgcc acgggttcca gccacatcat tattaaattt gccatcgtca ttaactttca
    65521 catggttaga atcggcgtat tcctgacgca cggcggcttt cagccagggt tccaacgtcg
    65581 taccgttttg caggtccatg tgatagctta ccgccgttcc cgcttcagcg cgtaatatcc
    65641 gggtatttcc cacatccgcg cgcatgccgt ttgataacgt gtagtcctga ccatctgtgg
    65701 taaagccggt aaaggcgaga tagggtctaa cactccacaa tccgtcaacc caacggaacc
    65761 cgctctcaac atgagcaccc gcgccgttac tattgtaatc gccaaacgct gttgccccat
    65821 tactcatctt gccatggatg gtgttggcaa aacggtcaac tttcaccacc ccatcaacat
    65881 aggcaccgtt ctgatgctcc caaccggcat aagcccccag ggtgtagcta tcaatattgc
    65941 ctttgccgcc gcgatcaaaa ccaatatcag aatgagagta atcaaagaac aagccgcgaa
    66001 ttgtgctgct ttcttcacgg gagaaacggc tatcgatacc gagcgtcagg cccgtcaatg
    66061 tttgctcaaa gccagctccc gcatcagtgg tcacgttgtt gcgggtgtta attgccgaac
    66121 tccacatcgc cgtatcgtaa ttaacgcctt ttacgctacc aagacgctca cgcacggtgt
    66181 ccagttctgc atcaaatacc agcggttgtg ctgctgccat gttcagcaca tcagtggttg
    66241 aaggggtaat ttgcgcgcga ttctctgcga ggctccagct atggttgcca ttatccagca
    66301 aggtatattc atacgtaccg atatcaacaa cgcctccggc attgcccaac gtgaatgcag
    66361 catcaccgcc gcccgtcgtt accagtgtaa ggctatctcc tgctgccggg ctggcccccg
    66421 tgtccgtcac gaatattttg aaatcacctg ttgcctgacc gatgacgttg agctgatcgc
    66481 tctgatgatt agccatatcg gtacgcatat aaaattgccg tttcccgaca gggtattggt
    66541 ggtcaacgta atgaaatttt ccgcacttgc cgtcacagat gaacgagtca ctggctcagc
    66601 catcataatc acatttgcgc cggacatcgc aaggttggct aagttgaggc gagtgttttg
    66661 gttcgtcgta gtgatatcgg caatgatggt ccatgcgcta ttttcgagcg aaacgtccgc
    66721 ttggctggta tccacggcac catgcgttct aaaagaagcg ccctcagaaa ccactacctt
    66781 acccaccaac atagcgggtg cataatttat tttcggcttc atgacctcaa gaatgccatc
    66841 attccctctg actgacacgt taaccattgt gccagcccag acattagcgc ggccattgtt
    66901 gataaccata ttttcagaga tggctttaga ggaatacgtg atacttccat tctgatctga
    66961 tcgaccgaga tcatatacag catttgcctg cactttcgta ccagtatcct ttcccagcga
    67021 ttgcatagtg gcatgctcat caaggatagt gtcaatggcc tgagtgtcct ccataacaat
    67081 gagcccggag ccatcatcca gttcataatt ttttgacaca ccatccttta tactaaattg
    67141 gcctttactg tttgtaccac tcacttccag cgcattcgtt gagacaatta atttcccgcc
    67201 tgctttttta tcaacgccag ttgcagtccc gccatccata acctccagta atcctccctt
    67261 ttcgatagcg gcaaagacgg caatcccatc tttttcaaca attaagcgcc cgcctttaat
    67321 cgtgggatca ttcgccgc
//
LOCUS       DAJVRF010000004        64212 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJVRF010000004 DAJVRF010000000
VERSION     DAJVRF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64212
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.004"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..1271)
                     /gene="eno"
                     /locus_tag="OQN13_000227"
     CDS             complement(<1..1271)
                     /gene="eno"
                     /locus_tag="OQN13_000227"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012005243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCS1613699.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAADKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNG"
     gene            complement(1359..2996)
                     /gene="pyrG"
                     /locus_tag="OQN13_000228"
     CDS             complement(1359..2996)
                     /gene="pyrG"
                     /locus_tag="OQN13_000228"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCS1613700.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3224..4015)
                     /gene="mazG"
                     /locus_tag="OQN13_000229"
     CDS             complement(3224..4015)
                     /gene="mazG"
                     /locus_tag="OQN13_000229"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCS1613701.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4086..4421)
                     /gene="mazF"
                     /locus_tag="OQN13_000230"
     CDS             complement(4086..4421)
                     /gene="mazF"
                     /locus_tag="OQN13_000230"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCS1613702.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4421..4669)
                     /gene="mazE"
                     /locus_tag="OQN13_000231"
     CDS             complement(4421..4669)
                     /gene="mazE"
                     /locus_tag="OQN13_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCS1613703.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4747..6981)
                     /locus_tag="OQN13_000232"
     CDS             complement(4747..6981)
                     /locus_tag="OQN13_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCS1613704.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7029..8330)
                     /gene="rlmD"
                     /locus_tag="OQN13_000233"
     CDS             complement(7029..8330)
                     /gene="rlmD"
                     /locus_tag="OQN13_000233"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCS1613705.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLCLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8387..11143
                     /gene="barA"
                     /locus_tag="OQN13_000234"
     CDS             8387..11143
                     /gene="barA"
                     /locus_tag="OQN13_000234"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCS1613706.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11375..12715)
                     /gene="gudD"
                     /locus_tag="OQN13_000235"
     CDS             complement(11375..12715)
                     /gene="gudD"
                     /locus_tag="OQN13_000235"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS1613707.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12736..14076)
                     /locus_tag="OQN13_000236"
     CDS             complement(12736..14076)
                     /locus_tag="OQN13_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS1613708.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14078..15430)
                     /gene="gudP"
                     /locus_tag="OQN13_000237"
     CDS             complement(14078..15430)
                     /gene="gudP"
                     /locus_tag="OQN13_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCS1613709.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSHIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15865..16314)
                     /locus_tag="OQN13_000238"
     CDS             complement(15865..16314)
                     /locus_tag="OQN13_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCS1613710.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16332..17114)
                     /gene="truC"
                     /locus_tag="OQN13_000239"
     CDS             complement(16332..17114)
                     /gene="truC"
                     /locus_tag="OQN13_000239"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCS1613711.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17114..17443)
                     /locus_tag="OQN13_000240"
     CDS             complement(17114..17443)
                     /locus_tag="OQN13_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCS1613712.1"
                     /translation="MTTHDRVRLQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18065..18610)
                     /gene="syd"
                     /locus_tag="OQN13_000241"
     CDS             complement(18065..18610)
                     /gene="syd"
                     /locus_tag="OQN13_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCS1613713.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18678..19526
                     /gene="queF"
                     /locus_tag="OQN13_000242"
     CDS             18678..19526
                     /gene="queF"
                     /locus_tag="OQN13_000242"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCS1613714.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19638..21002
                     /gene="ppnN"
                     /locus_tag="OQN13_000243"
                     /gene_synonym="ygdH"
     CDS             19638..21002
                     /gene="ppnN"
                     /locus_tag="OQN13_000243"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCS1613715.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21558..22847
                     /gene="sdaC"
                     /locus_tag="OQN13_000244"
     CDS             21558..22847
                     /gene="sdaC"
                     /locus_tag="OQN13_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCS1613716.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22905..24272
                     /gene="sdaB"
                     /locus_tag="OQN13_000245"
     CDS             22905..24272
                     /gene="sdaB"
                     /locus_tag="OQN13_000245"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCS1613717.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNASRMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24384..25139
                     /gene="xni"
                     /locus_tag="OQN13_000246"
     CDS             24384..25139
                     /gene="xni"
                     /locus_tag="OQN13_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCS1613718.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25244..26392)
                     /gene="fucO"
                     /locus_tag="OQN13_000247"
     CDS             complement(25244..26392)
                     /gene="fucO"
                     /locus_tag="OQN13_000247"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCS1613719.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMHYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26420..27067)
                     /gene="fucA"
                     /locus_tag="OQN13_000248"
     CDS             complement(26420..27067)
                     /gene="fucA"
                     /locus_tag="OQN13_000248"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCS1613720.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVATLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            27614..28930
                     /gene="fucP"
                     /locus_tag="OQN13_000249"
     CDS             27614..28930
                     /gene="fucP"
                     /locus_tag="OQN13_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCS1613721.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQRLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            28963..30738
                     /gene="fucI"
                     /locus_tag="OQN13_000250"
                     /pseudo
     CDS             28963..30738
                     /gene="fucI"
                     /locus_tag="OQN13_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            30847..32265
                     /gene="fucK"
                     /locus_tag="OQN13_000251"
     CDS             30847..32265
                     /gene="fucK"
                     /locus_tag="OQN13_000251"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCS1613722.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            32267..32689
                     /gene="fucU"
                     /locus_tag="OQN13_000252"
     CDS             32267..32689
                     /gene="fucU"
                     /locus_tag="OQN13_000252"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCS1613723.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            32747..33478
                     /gene="fucR"
                     /locus_tag="OQN13_000253"
     CDS             32747..33478
                     /gene="fucR"
                     /locus_tag="OQN13_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCS1613724.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(33522..34622)
                     /gene="rlmM"
                     /locus_tag="OQN13_000254"
     CDS             complement(33522..34622)
                     /gene="rlmM"
                     /locus_tag="OQN13_000254"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCS1613725.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(34615..35010)
                     /locus_tag="OQN13_000255"
     CDS             complement(34615..35010)
                     /locus_tag="OQN13_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCS1613726.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(35029..35946)
                     /gene="gcvA"
                     /locus_tag="OQN13_000256"
     CDS             complement(35029..35946)
                     /gene="gcvA"
                     /locus_tag="OQN13_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCS1613727.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(36297..36524)
                     /locus_tag="OQN13_000257"
     CDS             complement(36297..36524)
                     /locus_tag="OQN13_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCS1613728.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            36716..37921
                     /gene="csdA"
                     /locus_tag="OQN13_000258"
     CDS             36716..37921
                     /gene="csdA"
                     /locus_tag="OQN13_000258"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCS1613729.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            37921..38364
                     /gene="csdE"
                     /locus_tag="OQN13_000259"
     CDS             37921..38364
                     /gene="csdE"
                     /locus_tag="OQN13_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCS1613730.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(38415..39221)
                     /gene="tcdA"
                     /locus_tag="OQN13_000260"
     CDS             complement(38415..39221)
                     /gene="tcdA"
                     /locus_tag="OQN13_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCS1613731.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(39298..40395)
                     /gene="mltA"
                     /locus_tag="OQN13_000261"
     CDS             complement(39298..40395)
                     /gene="mltA"
                     /locus_tag="OQN13_000261"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCS1613732.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            40604..40680
                     /locus_tag="OQN13_000262"
     tRNA            40604..40680
                     /locus_tag="OQN13_000262"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40638..40640,aa:Met,seq:cat)
     gene            40714..40790
                     /locus_tag="OQN13_000263"
     tRNA            40714..40790
                     /locus_tag="OQN13_000263"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40748..40750,aa:Met,seq:cat)
     gene            complement(40865..42118)
                     /gene="amiC"
                     /locus_tag="OQN13_000264"
     CDS             complement(40865..42118)
                     /gene="amiC"
                     /locus_tag="OQN13_000264"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCS1613733.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            42350..43681
                     /gene="argA"
                     /locus_tag="OQN13_000265"
     CDS             42350..43681
                     /gene="argA"
                     /locus_tag="OQN13_000265"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCS1613734.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(43743..45569)
                     /gene="recD"
                     /locus_tag="OQN13_000266"
     CDS             complement(43743..45569)
                     /gene="recD"
                     /locus_tag="OQN13_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCS1613735.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEESLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKRRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(45569..49111)
                     /gene="recB"
                     /locus_tag="OQN13_000267"
     CDS             complement(45569..49111)
                     /gene="recB"
                     /locus_tag="OQN13_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCS1613736.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EVDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(49104..51992)
                     /gene="ptrA"
                     /locus_tag="OQN13_000268"
     CDS             complement(49104..51992)
                     /gene="ptrA"
                     /locus_tag="OQN13_000268"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCS1613737.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(52168..55536)
                     /gene="recC"
                     /locus_tag="OQN13_000269"
     CDS             complement(52168..55536)
                     /gene="recC"
                     /locus_tag="OQN13_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCS1613738.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(55549..55872)
                     /locus_tag="OQN13_000270"
     CDS             complement(55549..55872)
                     /locus_tag="OQN13_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCS1613739.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(55857..56264)
                     /locus_tag="OQN13_000271"
     CDS             complement(55857..56264)
                     /locus_tag="OQN13_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCS1613740.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(56261..56824)
                     /locus_tag="OQN13_000272"
     CDS             complement(56261..56824)
                     /locus_tag="OQN13_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCS1613741.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(56815..57285)
                     /locus_tag="OQN13_000273"
     CDS             complement(56815..57285)
                     /locus_tag="OQN13_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCS1613742.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(57470..58264)
                     /gene="thyA"
                     /locus_tag="OQN13_000274"
     CDS             complement(57470..58264)
                     /gene="thyA"
                     /locus_tag="OQN13_000274"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCS1613743.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(58271..59146)
                     /gene="lgt"
                     /locus_tag="OQN13_000275"
     CDS             complement(58271..59146)
                     /gene="lgt"
                     /locus_tag="OQN13_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCS1613744.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(59297..61543)
                     /gene="ptsP"
                     /locus_tag="OQN13_000276"
     CDS             complement(59297..61543)
                     /gene="ptsP"
                     /locus_tag="OQN13_000276"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1613745.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(61556..62086)
                     /gene="rppH"
                     /locus_tag="OQN13_000277"
     CDS             complement(61556..62086)
                     /gene="rppH"
                     /locus_tag="OQN13_000277"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCS1613746.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            62771..63460
                     /gene="mutH"
                     /locus_tag="OQN13_000278"
     CDS             62771..63460
                     /gene="mutH"
                     /locus_tag="OQN13_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCS1613747.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            63529..>64212
                     /locus_tag="OQN13_000279"
     CDS             63529..>64212
                     /locus_tag="OQN13_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS1613748.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLN"
BASE COUNT        15543 a        17297 c        15741 g        15631 t
ORIGIN      
        1 ccgttgtacg gtgctttttc gcccagagct tcttcgatac gaatcagctg gttgtattta
       61 gcaacacggt cagaacggct catagaacca gttttgatct ggcctgcagc agtaccaaca
      121 gccaggtcag cgatggtagc gtcttcagtt tcgccagaac ggtgagagat aactgcagtg
      181 tagccagcat ctttcgccat cttgattgca gccagagttt cggtcagaga accgatctgg
      241 ttgaatttga tcaggatgga gttagcgata cctttttcga taccttcttt caggatcttg
      301 gtgttggtta cgaacaggtc gtcaccaacc agctggattt tgtcgcccag aactttggtc
      361 tggtatgcga aaccgtccca gtcagattcg tccagaccgt cttcgataga tacgatcgga
      421 tactgtttgg tcagttcttc caggaagtga gtgaattctt cagaggtgaa agctttgttg
      481 ccttcgccag ccagaacgta tttaccgtct ttgtagaatt cagaagctgc gcagtccatc
      541 gccagagtga tgtctttgcc cagttcataa ccagcagctt taacagcttc agcgataaca
      601 gccagagctt cagcgttgga acccaggttc ggcgcatagc caccttcgtc accaactgca
      661 gtgttcatgc ctttcgcttt cagaactttt gccaggtgat ggaaaacttc agaacccatg
      721 cggatggctt ctttcacagt tttcgcgcca accggctgaa tcatgaattc ctggatatca
      781 acgttgttgt cagcgtgctc accaccgttg atgatgttca tcatcggaac cggcatggag
      841 tatttgcccg gagtgccgtt cagttcagcg atgtgctcgt acagcggcat ccctttatcg
      901 gcagcagcag ctttggcgtt agccagagat acagccagga ttgcgttcgc gccgaatttg
      961 gatttgtttt cggtgccgtc caggtcgatc atgatcttgt caatgccagc ctgatcttta
     1021 gcgtctttgc caatcagtgc ctgagcgatc gggccgttta ccgcagcaac agctttggtt
     1081 acgcctttac ccaggaaacg ggatttgtcg ccatcgcgca gttccagagc ttcacgggaa
     1141 ccagtagaag cacctgacgg agcagctgcc ataccgacga aaccaccctc cagatgtact
     1201 tcggcttcaa cagtcgggtt accacgggag tcgatgattt cacgaccgat gatttttacg
     1261 attttggaca ttaggttttc ctcaagtcac tagttaaact gaaactccag acaaacaacg
     1321 cgtacccaag gtacgcgttg ccgttctaac ttttttactt acttcgcctg acgtttctgg
     1381 aactcgctgg cagctttcac aaagcctgca aacagcgggt gaccatcacg tggagtagaa
     1441 gtaaactccg gatggaactg gcaagccacg aaccacgggt gattcggaac ttcgatgatc
     1501 tcgaccaact gatcatcccc ggaacggccc gcaacgcgca gacctgcatc ttcaatctgt
     1561 ttcaacagca tgttgttgac ttcgtaacgg tgacgatgac gctcaacaat tgtcggcgca
     1621 ttgtacagct ggcgaaccag gctatcgtca accaactggc actgctgtgc gccgagacgc
     1681 atggtaccgc cgagatcgct cttctcgcta cgaacttcaa cgttgccgtt ttcatcgcgc
     1741 cactcggtaa tcagcgccac aaccgggtac ttacagtctg gcacaaattc cgtagagttg
     1801 gcgttctcca tgttggcaac atggcgagcg taatcaatta acgccacctg catacccagg
     1861 caaatgccca gataaggaat attgttctca cgcgcaaaac gcgcggtcgt aatcatgcct
     1921 tctacgccac gatagccgaa accgccaggt acgaggattg cgtccagacc tttaaggatt
     1981 tcaacgccgc gcgtttcaac atcttgtgaa tcgatcagtt tgatgttgac gctgacacga
     2041 ttcttcagcc caccgtgttt cagtgcttcg atcactgatt tataagcatc cggcagttca
     2101 atgtacttgc cgaccatacc gatggtgact tcacttaccg gattcgcttc ttcgaagata
     2161 acctgttccc attcggacag atttgcttcc gggcagttta agctgaatcg tttacaaata
     2221 taatcgtcca gcccctgaga tttcaacagg cccggaattt tatagatgga atcgacgtct
     2281 ttcagagaaa taaccgcttt ttccggaaca ttacagaaca atgcaatctt cgcacgttcg
     2341 ttcgccggaa cagcgcgatc tgaacgacaa atcaggatgt caggctggat accgatggag
     2401 agcagctctt ttacagagtg ctgagtcggt ttggttttga cttcaccaga cgctgccatg
     2461 tacggcacca gcgtcaggtg cataaacaga gtgtgctcac ggccaatttc aacagccatc
     2521 tggcgaatcg cttcgaggaa cggcaaggat tcgatatcac ctactgtacc gccgatttct
     2581 accagtacta cgtcatgacc ttcgccacct tccagcacgc gctctttgat tgcgttagtg
     2641 atgtgcggaa taacctgcac ggttgcgccg aggtagtcac cgcggcgttc tttacgcaga
     2701 acgtcagagt agatacgacc cgtggtgaag ttgttgcggc ggctcatttt ggtacgaatg
     2761 aaacgctcgt agtgccccag gtccaggtcg gtttcagcgc cgtcttcagt aacgaacact
     2821 tccccgtgtt ggattgggct catagtacct ggatcgacgt tgatgtacgg atccagtttc
     2881 atgatggtca cattgaggcc acgggcttca agaatggctg cgagggaggc tgcggcaatg
     2941 cctttaccca gagaggatac gaccccgccg gtcacaaaaa tatagttcgt tgtcatgctg
     3001 aacctgagaa gttaggttga aaagacgatg gaataaccag gacgggaaag cagtataccc
     3061 gaacatgacc tgtgccacaa actttcatta tccctcctct tcgccagcgc actattgaaa
     3121 tcaggagtga gaaaatagcc cctttggggt aaatgttttt gacgcaaatc aagcgcttgt
     3181 catttaaaaa atgacacaaa tggcgcttga ccgcgtaatt cccttagaga tcaatttcct
     3241 gccgttttac ctgttgccag acttcttcca ttgtttcgag gtcaacacct gtcatttcca
     3301 gtccacgcgc ggcaacaata cgctccactt cgcgaaaacg acgctcgaat ttttcgttcg
     3361 ctttttgcaa tgcgatttct gctttcgtcc ctaaatggcg agccagatta accgtggcaa
     3421 acagcaggtc ccccatttcc tcctccagtt tagcctggtc gacaacagcc tgccgcgcct
     3481 cgtacatcac ctcgtcgatc tcttcgtaga ctttatcaac caccgggcca agcgtcgtcc
     3541 agtcgaagcc aacgttggcg caacgtttct ggattttttg cgcccgcatt aaagccggta
     3601 aactacgagg aatatcgtcc agcgccgaat gctgcgcttt ctgcgcgcgc tcttcggttt
     3661 tgatttgctc ccaacgggca agcacttcac tactgttttc ggcagaacta tcagcaaaaa
     3721 catgcggatg gcgacgctct aatttatcgc taatagcagc gcaaatatca ttaaagtcaa
     3781 agcgcccttc ttcctgagcc atttgcgcgt aaaacaccac ctggaatagc agatcgccca
     3841 gttcaccgcg aagatcgtca aaatcttcac gggcgatggc gtccagcact tcgtaggttt
     3901 cttcaagggt gtaaggcgca atggtggcaa atgtctgctc tttatcccac gggcagccgt
     3961 tttccggatc gcgcaggcgc tgcataatag tgagcaaacg gtcgatttga ttcattgaat
     4021 tgtcctgaaa attgcgggtc tgtcaggtgg aaacctgtga ccagaataga agtgagttag
     4081 taacactacc caatcagtac gttaattttg gctttaatga gttgtaattc ctctggggca
     4141 actgttcctt tcttcgttgc tcctcttgcc cgccaggcga tactttttac ctgatcagct
     4201 aacgctacgc catcacgttc ctgaccggat aaaacaactt cgaacggata tccttttgat
     4261 tgcgttgtac aaggaacaca cagacacata cctgttttgt tgttgtacat gaaaggactc
     4321 aggacaacag ctggacgatg tccagcttgc tcgctacctt ttgtcgggtc aaaatcaacc
     4381 caaatcagat cgcccatatc gggtacgtat cggcttacca ttaccagact tccttatctt
     4441 tcggctctcc ccagtcgata ttctcgtgga ggttttccgg cgtgatgtcg ttgaccagtt
     4501 cagcaagcgt aaatacgggc tctttacgca ctggctcaat aattaatttg ccatccacca
     4561 ggtcaatctt cacttcatca tcaatattca gattgagcgc ctgcattaac gtagccggga
     4621 tccgcaccgc cggtgaattt ccccaacgct ttacgctact gtggatcata accctttcct
     4681 caaaccgcta tcatatgtag atacagtata tatcaatcta cattgtagat acgagcaaat
     4741 ttctgcctaa ctcccgtgca accgacgcgc gtcgataaca tccggcacct ggttgagttt
     4801 acccagcacg cggcccagca cctgcaggtt gtaaatctca atggtcatgt cgatggttgc
     4861 cagttgctgt tttgtgtcgc tacggctggc aacgccaagc acgttcacct tctcgttggc
     4921 gagaatggtc gtgatatcac gtaacaaccc actacgatca ttagccacca cgcggaccac
     4981 cagcgaatat ccggcggagt agctctcacc ccataccgcg tcaacaatgc gttctggcgc
     5041 atgagagcgc agttccgcca gttgttcgca atcggcgcgg tgtactgaaa taccgcgccc
     5101 ctgggtaatg aagccgacaa tctcatctcc aggaatcggc tggcagcagc gcgcgatgtg
     5161 gtgcatcagg ttgccaacac cttcgactac cacgcgaccg ttatctttac tgcggttttg
     5221 cggcgtgtag cttttttgct gaagttgctt cagcgcggcg gcgtcctgct cttcggcact
     5281 cggcttatta aattgcgatt gcaggaagtt caccatctga ttgagacgga tatccccgcc
     5341 accaatcgcc gccagcaact cgtcgacatc attaaagttg taacgcggca gcagatgttt
     5401 ttctgcttct ttcaggctga tccccagatg ttccagctcg tcgtcaagga tttgccgccc
     5461 agccagaatg tttttgtcac ggtcctgttt acggaaccaa gcgtgaattt tcgaacgccc
     5521 acggctggtt gtgacgtaac cgaggtttgg gtttaaccag tcacggctgg ggttcggctg
     5581 tttctgggtg ataatttcaa tctggtcgcc catctgcagc tggtaggtga acggcacaat
     5641 gcgcccgcca atttttgccc cgatgcagcg gtgtccgaca tcactgtgga tgtggtaagc
     5701 gaagtccagc ggcgttgatc ccgcaggcaa atcaacgaca tcacctttcg gcgtaaagac
     5761 atacacccgg tcgtcaaaga cctggctgcg tacttcgtcg agcatttcgc cggaatcagc
     5821 catctcttcc tgccacgcaa tcagtttacg cagccaggca atccggtctt catgtcccga
     5881 acgtgcgccg ccagctgccg cgccctcttt atatttccag tgcgcagcaa cacccaactc
     5941 tgcatcttca tgcatctgtt tggtgcggat ttggatctca acggttttgc cacctggccc
     6001 cagaaccacg gtatgaatag actgataacc gtttggtttc gggttagcga cataatcgtc
     6061 aaactcatcc ggcaggtggc gatagtgagt gtgcactatc cccagtgcgg catagcaatc
     6121 ctgtaaacgc tcggcgacaa tacgtaccgc acgcacatca aacagctcat caaaggcgag
     6181 gttctttttc tgcattttac gccagatgct gtagatgtgt ttcggacgac catacacttc
     6241 cgctttaacg ccttcagctt tcatctcagc gcgcagatga ccaacgaact cttcgatgta
     6301 gtgttcgcgg tcgagacgcc gttcatgcag cagtttggca attcgtttgt attcggttgg
     6361 atggaggtaa cggaagcagt aatcttccag ttcccatttc agttgtccga ttccgagacg
     6421 gttagccagc ggtgcgtaga tgttggtaca ctcttttgcc gccagtacac gttcatcttc
     6481 cggcgcatct tttacttcgc gcagatgagc aatacgctcc gccagtttga tgactacgca
     6541 gcgaaaatca tcgaccatcg ccaataacat ccggcgaacg ttatcgacct gttcggagga
     6601 aacagaatca gtgtgcgtcg ctttcagctg gcggatcgcc gccatatcac gcacgccgtg
     6661 aataaggtta acaaccgact taccgacgct ctcacgcagc acatcttcgc tgactacgtt
     6721 ggcatcagcc agagggaaca gcagcgccgc ccgcagcgtg tcaatgtcca tacttaatgt
     6781 cgagaggatc tccaccatct caacaccacg ccacaataac agactggcat ccggatgccc
     6841 ctgcgtctgt tgcagacaat acgcccaagt ttcggctaag cactcacacg acttctggct
     6901 ggtaataccc agacttgcga tccatttttc cggatcaaat tcaccagcct tattgatatg
     6961 tgcacttctt accgcaacca tcgtcctctc ctttagggac cagacctgcc gaaatcggca
     7021 aatcgcaact atttaacgcg cgagaaaagt accatcgatt ccagatgtcc cgtgtgtggg
     7081 aacatatcca gcatcgccag tcgcgcaatg gtatatcctg cttttaataa cgcttcgcta
     7141 tcccgagcca gcgttgcagg gttacaggat acataaacta tacgaatagg ttccagtttt
     7201 ataatttgct gcataacacc tgcggcacct gctcgcgccg ggtccagcaa cactttatcg
     7261 aagccgtttt tcgcccacgg ctgctttgtg acatcttctt caagattttc gtgataaaac
     7321 gtcacattct gtaagccatt aagacgcgca ttctgctggc ctttttccac cagcgccgga
     7381 acgccttcta cacccaccac actggcagct tgtgtcgcca atggcagtgt aaagttgccc
     7441 ataccgcaga acaaatccag tacgcgatct tcaggttgca catccagcca ttccaacgca
     7501 cgcgctacca ttttttggtt cacacccgca ttgacctgaa taaaatcgcg cgggctaaaa
     7561 gttaagcaca acccatttga gtcataccag ggcatctcac cagagacggt ttcgagtatc
     7621 tcactatcgg gggcgagata cagatccagg ccttcagaat gcgaaaagcg ttccagtttt
     7681 tcgcgatctg ccgaacttag cggtgcggta tggcgcaaaa tcatcagcgt gccgctggtt
     7741 gcctgtacca gttcaacatg accaagatgg cgcataactt gtaagctgcc caagcatgcc
     7801 atgactttgg gcagcaatgc ttcaagttgg ggcactaaaa tggggcattg tttaacgtcg
     7861 acaatgtcac tggagcccgc tttgcgaaac cccatctgaa gttgctgtgt tttcggtaag
     7921 tagttcagac ttaaacgcgc gcgacggcga tagccccagg gaacatcggc gatcacttca
     7981 gagacttcgt gtttcattaa tcgggcgagt gccgcacttt tgcttcgctg ctgtaaatcc
     8041 acgctggcgt gttgttgctg acagccgccg catacgccaa aatgaggaca gcgtggcgtt
     8101 tcgcgtcccg ggctatcgct taaccggcgt acgactttag cgcgggcata ctgtttttta
     8161 tcttcagtaa cagtaacttc cgcgttttcc tgcggcaata atccggggat aaatagcgtt
     8221 ttgccgttat gtcgcgccac gccctgacca aaagagtcga ggtcgttgac tgaaacggtt
     8281 atgatctgac gcgtcgtcgt gcgtcgtttt gcagagtaga attgcgccat tggcgagact
     8341 ttctcaattt aacagtgtga ccttaattgt cccataacgg aactccatga ccaactacag
     8401 cctgcgcgca cgcatgatga ttctgatcct ggcaccgacc gtccttattg gtttattgct
     8461 gagtatcttt ttcgttgtgc atcgctataa cgacttgcag cgtcaactgg aagatgccgg
     8521 agccagcatt attgagccgc ttgcagtttc tactgaatat ggcatgagcc tgcaaaatcg
     8581 cgaatctatc ggtcagttaa taagcgtact gcatcgtcgc cattccgata ttgttcgcgc
     8641 gatttcggtt tatgatgaaa ataaccgact ctttgtcacc tccaattttc atcttgatcc
     8701 ctcatcaatg cagctcggca gcaacgtgcc gtttcctcgc cagctcactg tcactcgtga
     8761 cggcgatatt atgatcctcc gcacgccgat tatttctgag agttactccc ccgacgaatc
     8821 gcccagtagc gatgccaaaa atagtcaaaa tatgttggga tatattgcgc tggagctgga
     8881 tcttaaatcg gttcgcttgc agcaatataa agagatcttt atttccagcg tgatgatgct
     8941 gttttgtatc ggtattgcgc ttatttttgg ctggcgctta atgcgcgatg taaccggtcc
     9001 gattcgcaac atggtgaata ccgtcgaccg catccgtcgc gggcaactcg acagccgggt
     9061 ggaaggattt atgctcggcg agctggatat gctgaaaaac ggtatcaact cgatggcaat
     9121 gtcgctggct gcttatcacg aagagatgca gcacaatatc gaccaggcga cgtccgatct
     9181 gcgtgaaacg ctggagcaga tggaaattca gaacgttgag ttagatctgg cgaaaaagcg
     9241 cgcccaggaa gcggcgcgta ttaaatccga gtttctggca aatatgtcac acgagctgcg
     9301 tacaccactg aatggtgtta ttggctttac ccgcctgacg ctgaaaacag aattaacacc
     9361 aacgcagcgc gatcacctga atacgattga acgttcggca aataatttgc tggcaattat
     9421 taatgatgtt ctcgacttct cgaaactgga agcaggtaag ctgattctgg aaagtattcc
     9481 attcccacta cgcagcacgc tggatgaagt cgttactctg ctggcacatt cttctcacga
     9541 taaagggttg gaactgacgc tcaatattaa aagcgacgtg cctgataacg tgatcggcga
     9601 cccactgcga ttacagcaaa tcatcactaa cctggtgggg aatgcaatta aattcaccga
     9661 gaatggcaac attgatattc tggtagaaaa acgtgcgctg agtaatacca aagtgcagat
     9721 tgaagtgcag attcgggata ccggcattgg tattcctgaa cgcgatcaat cgcgcttatt
     9781 ccaggccttc cgacaggctg atgccagtat ttcccgccgt catggtggca ccggtctggg
     9841 gctggtgatt acacaaaaac tggttaatga aatgggcggc gatatttcgt tccatagcca
     9901 gccgaatcgc ggttcaactt tctggttcca cattaatctc gatctgaacc cgaacattat
     9961 tatcgaaggg ccatccaccc agtgcctcgc cggtaagcgc ctggcctatg tcgaaccaaa
    10021 ctccgcagca gcgcaatgca cgctggatat tttaagtgaa acgccgctgg aagtggttta
    10081 tagcccaacg ttctccgcgc tgcctcccgc gcattacgac atgatgttgt taggcatcgc
    10141 ggtgaccttc cgcgagccgc taacaatgca acatgagcga ttagcgaaag cggtatcgat
    10201 gaccgatttc ctgatgctgg cacttccttg ccatgcacaa gtcaatgcag aaaaactcaa
    10261 gcaagatggt atcggcgcgt gtctgctgaa accattaaca cctacgcgcc tgttgcctgc
    10321 cctgacggaa ttttgtcatc acaaacaaaa cacgcttttg cctgtaaccg atgaaagcaa
    10381 gctggcaatg acagtcatgg cagttgatga caaccccgct aacctgaaac ttatcggcgc
    10441 attgctggaa gatatggtgc aacatgtgga actttgcgat agcgggcatc aggcggttga
    10501 acgggcgaaa cagatgccgt tcgatttgat cttaatggat attcaaatgc ctgacatgga
    10561 tggcattcgg gcctgcgagc tcatccacca gctcccccat cagcaacaaa cgccggttat
    10621 cgcggtaacg gcgcatgcaa tggccgggca aaaagagaag ctgcttggcg cagggatgag
    10681 cgattatctg gcgaaaccga ttgaagaaga gcgattgcat aatttgttgt tgcgctacaa
    10741 gcctggcagc ggtatttcct ctcgcgtcgt gacgcccgaa gtcaacgaaa ttgtggtgaa
    10801 cccgaatgcg accctcgact ggcaactggc actacgccag gcagcaggaa aaaccgatct
    10861 agcgcgcgat atgctgcaaa tgttactcga tttcctgcct gaagttcgca acaaagttga
    10921 ggagcagctg gttggagaaa acccggaagg cctggttgat ttgattcata aactgcatgg
    10981 cagttgcggc tatagcggtg tgccgcgtat gaagaatctc tgccaactga tcgaacaaca
    11041 gctacgtagc ggtactaaag aagaagattt ggagccggag ctgctggaac tgttggacga
    11101 gatggataat gtcgcgcgcg aagccagcaa aattctcggg taacggacgt cttgtcgagt
    11161 agcaatcggc tttttatgac gctggtttca gattggtgac aaagtgcctg tgtttatgcc
    11221 ggatgcggcg ttaacgcctt atccggccta caaaaccaat taaattcaat gaattaaaaa
    11281 atgatgtaga cctgatacat agcacaacag gctattttgc gtttagcatc agtctcaagc
    11341 cggctccaga tagagccggt tttggttttc tgtcttaacg caccatgcac gggcgcttgt
    11401 tatcgaacgt ccagccagga atcagatact gcattcccat cgcatcgtca cgcgcgccaa
    11461 gcccgtgttt ctgatacagc tcatgggctt tcatcacttg atccatatcg atttctacac
    11521 ccagccccgg tttttccggc acctgaacca gaccgccttt gatctcaaac ggttctttgg
    11581 tcaagcgctg attgccttcc tgccaaatcc agtgcgtatc aatcgcggtg attttacccg
    11641 gtgcagcggc ggcaacatgg gtaaacatcg ccagggaaat atcgaagtgg ttgtttgagt
    11701 gtgaacccca ggtcaggcca aattcatggc acatttgcgc cacacgtacc gaaccttgca
    11761 tagtccagaa atgcggatcc gccagcggga tatcaacgga ttgtaacgag agcgtatggc
    11821 ccatctgccg ccagtcggtg gcgatcatat tggttgcagt cggcaggcca gtcgcgcgac
    11881 ggaactctgc catcacttca cgcccggaga aaccttgctc cgcaccatac ggatcttctg
    11941 cataagccag cgaacctttc aggtatttac caattttaat tgcttcgttc agcgaccagg
    12001 caccgttagg atcgagcgta atacgcgcct gcgggaagcg ttgcgccagt gccacaatag
    12061 actcggcttc ttcttccccg gccagtacac cgcctttcag tttgaaatcg ttgaagccat
    12121 atttttcata cgccgcttcc gctaggcgca ccaccgcatc cggcgtcatc gcttcttcat
    12181 gacgcaggcg ataccagtcg catgagtcat ccggctggct ttgatacggc agaggtgtgg
    12241 ctttgcgatt accgacgaag aacagataac cgagcatttc gacttcgcta cgctgttgac
    12301 catcgcccag cagcgatgcc acgtttaccc ccagatgctg ccccagcaga tccagcattg
    12361 ccgcttctat cccggtaact acatgaatag tggtacgcaa atcaaatgtc tgcaaaccgc
    12421 gcccaccagc atcacgatcg gcaaaagtat tacgcaccag cgtcagaacg tttttgtatt
    12481 cacccagcgt tttacctacc accagcggaa tcgcatcttc cagcgtttta cggattttct
    12541 cgccgccggg aatttcccct acgccagtgt gaccggaatt atctttgata atcacaatat
    12601 tacgcgtaaa gaacggtgcg tgtgccccac tcagattcat cagcatactg tcatgacccg
    12661 ccaccgggat gacctgcatt tcagtaacaa cagacgtcgt aaattgagaa ctcatattta
    12721 tgtccttttt cagaatcaat gacggccgaa aacgggacgt ttacggtcaa aggtccagcc
    12781 ggggatcagg tactgcatcg gacctgcgtc gttacgcgca ccgccaggca gacgtttata
    12841 ggcctcatgt gccttttgta cctgttccca gtccagttcc acgcccagac cgggcgcatc
    12901 aggaacggca atttttccgt ttttaatctc cagcggattt ttggtcaggc gacaatcgcc
    12961 ctcctgccaa atccagtggg tatcgatagc ggtaggatta cccggtgccg ccgcgcccac
    13021 atgggtaaac atcgccagag agatatcgaa atggttatta gaatggcagc cccaggtcag
    13081 cccccagtcg tcgcaaagct gcgccacacg gactgcaccg gaaagcgtcc agaagtgcgg
    13141 atcggcaagt ggaatatcta ccgcattgag catcaccgca tgacccattt cgcgccagtt
    13201 ggtggcgatc atgttagtcg cgacgggcaa gccggtcgcc cgccgaaact ccgccataac
    13261 ttcacgaccg gagaaaccct gttctgcgcc gcatggatct tcggcatagg taagaacatc
    13321 attcagccct ttgcatagag aaatggcttc atcaagtagc caggcaccgt tgggatcaac
    13381 ggtaatccgc gcatcaggga agcgtttctt caatgcacga acagtgtcga tttcttgctc
    13441 gccaggtaac accccgccct taagtttgaa atctttaaag ccatagcgat cctgtgaggc
    13501 ttccgccaga cgcacaacgg cttcgctgtt catcgctttc tgatggcgca actgatacca
    13561 ctcatggttg cccggcgtat tttccagata aggaagatcg gttttggtcc gatcaccgat
    13621 ataaaacaga taaccgagga cggtaatagt ctcgcgttgc ttgcctggcc ccaacagttc
    13681 gcagaccgga acattcagcg ccttacctag caggtcaagc aaggcggctt ccagcgccgc
    13741 cacggcatta acgcgcaatt caaaagtcca ggcacctttg ccgaaggtat caaaatcggc
    13801 tgcctgatta cctttatgca cctgctgaac cactttattc aggcgcgcaa cttcctggcc
    13861 cagaaccatc ggaatagcat cgaccagtgt ttgataaatc acctctccgc ccggcgcttc
    13921 accaatgccg gtatgcccgg cgttatcggt gagtaccaca atattgcggg tgaaatatgc
    13981 gttatgtgcc ccaccaatat taagcaacat gctatcgtgc ccggcgaccg gaatgacttt
    14041 catatcagta ataacaggac tggattgtgt cgtcatctta ttgccccgcg acaggtttca
    14101 gctcgatacg cttgatatcg cccaccagca ccaggtagct cagtaccgcg attaaggcat
    14161 gaacaccaac ataaatcagc gccccattaa aggagccagt cgtgccaacg atataaccaa
    14221 ttgcgattgg cgtgacgatg ccagaaatgt taccgaacat gttgaacagg ccaccggaaa
    14281 gaccgctgat ctcttttggc gcggtatctg ccattactgc ccaacccagc gccccgatgc
    14341 ccttaccgaa gaaggccagc gccataaagc cgatgatcat ccactcaacg ttgacgtagt
    14401 tgcagaacac catcaccatc gacagcaaca tgcccattac gatcggtgtt ttacgcgcaa
    14461 tgttcagcga tcccgtgcga cgcatcagcc agtcggaaat aatcccaccc agcacaccgc
    14521 cgataaaacc gcaaaccgcc ggaacggaag ccacaaagcc cgctttcaga atcgacatcc
    14581 cacgcgcctg caccagataa accggaaacc aggtaatgaa aaagtaagtc agggcgttga
    14641 tacagtactg accgatataa acgccgatca tcatccgtga cccgagcagt tgtttgatct
    14701 gcccccactt cacgctgaac ggcactttaa ctttggtgtt ttgctgatcc atattgatca
    14761 gcgcgccacc cgcggcgatg tactccagct ctttctgatt tacccccgga tgttgatttg
    14821 gctcgtggat gactttcaac cagataaagc tgatgacaat ccccagaccg cccataaaga
    14881 agaagacgtg tgaccagccc acttcatgcg tcagccagcc cataatcggc gcgaagatca
    14941 ccgttgcgaa gtattgagcg gagttaaaaa tcgacaccgc cgttcccctt tcctgcgccg
    15001 gaaaccaggc cgcaacaatg tgactgttgc cggggaaaga tggcgcttca gcaagcccga
    15061 ccaggaagcg cagagtaaac agggcaacga taatgccgaa tccactaaag atatcgacga
    15121 agccttgcag caaggtaaac atcgaccaga taaagatcga ccagaagtag acgcgttttg
    15181 aaccaaaacg atccagcaac caaccaccag ggatctgccc gataacataa gcccatgaga
    15241 aggcagagaa cacatagccc attcccacgg gatcaaggcc gatatctttg gccatttccg
    15301 aaccggcaat agaaagcgta gcgcggtcgc cgtagttgaa ggatgtgacg ataaacaaca
    15361 tcaccactat ccagtaacga gcattcgtgc gtttttccac actgctcgca gcctgactta
    15421 aagaactcat tgttgcactc ctgaaaattc gcgttagcca cgctcactct ggactgcgac
    15481 atagccagga aatcagaggt gacgtagggt gttttttgcc gtttttatag gtcgttcgcc
    15541 gaatacggcg cgtgtttata tctggcaata gcagtataaa aagcgcgcca tagcggctca
    15601 ccgtgcaaca acacaacatt aatgcgttca atgaggcccg attttggcat tagccctgga
    15661 cggtggaatc cacttcacgg aaatgaaaac aagaacaaga aaggaagggt taaaacgaag
    15721 aaataaagaa gagtatgaaa tggatcgctt gactccaggc aaacgccagt aaaaatccgc
    15781 gctatgaagc agtttttact ggcatttgcc tgaaaagatt cgattcagca ccgctaaaac
    15841 gacatttacc gctcgctgaa catatcagga caacagcgtg ccccactgtt cgacccacgg
    15901 atttgattcc gtttccggtt ccgggttctc actggcgtca atcaacagca tttcgccaac
    15961 ccgctgagcg ctctgttcct gcaacaaggc atcgaactgt ttgccgccat tgcagaaatt
    16021 tacataactg ctgtcgccga gcgcaatcac gccataacgc agattcggct ggaagcccag
    16081 actatctttg attccctgaa agagcggcac aatgctatca ggaaggtcgc cctgcccggt
    16141 cgtggacgta accaccagaa catacttatc ctgataaggc agccagtcgc ttaattcagg
    16201 atcttcaaat accgttgctt tgtggccctg tgcggtcaga attgcttctg cttcttccgc
    16261 cactaacagc gaatttccgt acatggtgcc gacaaaaata ccaatttccg ccataccgtt
    16321 ttctccctgg attaggaact tatctctcca tcctgacccg atggcgcact gaactcaacc
    16381 ctttcatttt caggaagcag accgcgccag ccaaattgtg ataacgcctg catccaggtg
    16441 tcgtccaggc ccgcgtgaat agtcagcggc tcgccagtaa aaggatgcgt caatgacaac
    16501 tgactggcat gtagcattaa ccgctggagg ccaaaatgct cagcaccgct gcgattctgg
    16561 cgtaaatcgc catgtttgct atcgccaata atcggatgac gcaaatgggc aagatgtcgg
    16621 cgaagctgat gtttgcgtcc ggttttcggc tccagttcca ccaggccgta gcgcgtggtc
    16681 gggtaacgtc cggtcgctac cggcatttct acggtcgcca gaccgcgata atgcgtcact
    16741 gctggctgcg ggcctttatc ttcgcgggca aatttatcag cgattttgtc cagttcttcc
    16801 accagtggat aatccagcac cgcttcttcc atcaaccagc cgcgcacaat cgcatggtaa
    16861 cgtttctgga tttggtgctg ttcaaactgt tgtgccagca gccgtccggc ctcgctggat
    16921 aatcccatca acaacacacc agaagtgggt cggtccagac gatgagcagt aaaaacatgc
    16981 tgtcctatct ggtcacgcac ggtttgcatg accactactt tctcgtcgcg atccagccag
    17041 ctacggtgaa ccagccagcc ggagggttta tttaccgcaa ccagccattc atcctgatag
    17101 agtatttcca gcattagctc gcatcatccg caaaaagagc atccagtttt tccaactcag
    17161 ccagaataag cgcgcgttgc ggatggtccg tcgccagcgc catttcataa tagggtgcaa
    17221 cagcaaaagc gcccggtaac ggctggttgt tatttaacaa atcgtgcatt cgcgggatca
    17281 gcacccactg caaccactcc agtggttcca tggtgtccat aaagaacggt tgggtactat
    17341 taaattgatg cggctggggt tcatcgtttc gccagtgctg atgttcacgc agtaacgctt
    17401 caagcgtctg caactggaga cgaacgcggt catgagtggt cataaagcaa cttcaataag
    17461 aaaaactgcc gcgaaggata gcaggaataa aaaaagggag cactgtattc acagtgctcc
    17521 cggttcgttt cgcagcattc cagctacttt tgttgctccc tgctcatcct tgacaacttt
    17581 tcctctggcc ttgcggccaa tcgttcatcc tgaactattg cttcctgctc acaccacccc
    17641 gatgtgatac ttcatcctga agtgtccctg gccttcctga cccaccgaat catcctgacc
    17701 ggttctcatt ctccatcctg gaggtgtcct ttaacgcgtc ctgcgtcatc ctcttcgctt
    17761 catccagaag cctttccctg aaacaccatc ctggtgtgtc ctgcagaagt gtcatcatcc
    17821 tgatgttcac ttcgttgtct gactccctgt cgacgaagat agaatcgtct ttttcgcgaa
    17881 gtcttacaag gcgcttacag acaatgcctt aaggaaaatt tcatatgaaa gttaagaatt
    17941 aacaatcaac attttgattt taaatgaaaa taacgattct tatcttttta agtattcgct
    18001 tatttcctgg cgatctccta cagggtctgt aagagatctc tcacaaccag atggggtatt
    18061 tggattacag aagaggctta agttgattaa ggaattccgc aagatttgag gctaaatgcg
    18121 tccgtttacg tgttcccagc gtctctttgc acacttcacc ggagagatta cacaccgaga
    18181 tgacctctaa ctcttcttcc agcgtcgcga taaatagcgt gggcggcagt tttagccttt
    18241 tctgagtcac cagatgacca atcagatttt cctgcacccg acggaagtcg tcttcactcc
    18301 aggtttgcag caatgtcagt ttgatatcgc caaactgcgc gtgcatatcc ccggcaaact
    18361 gagtcgtata aaaggtatga attgtgggtt gtatcacaat atcaaaagcg cgttcaaccg
    18421 cgtttacatt ttgttccccg gtaaacggct ggggttgcca gtatacggca tcttcggtag
    18481 ttgaaataat gcacggtgaa ggaacaccgt acagttcctc acttaacggc cagcttttat
    18541 gctcttcatg ccacgcatcg cagtagcgtg cagtaaaatc tttcagggct tgtgcggtca
    18601 aatcgtccac taatttctct cttcacttaa accagataca cttgtcgttt agtttatctg
    18661 gtttatgacg gtgaaacatg tcttcttatg caaaccatca ggcacttgcg ggcctgactc
    18721 ttggaaaatc aaccgattac cgggatacct atgacgccag cctactgcaa ggcgttccac
    18781 gcagcctgaa tcgcgacccg ctgggtctga aagcggataa cctgcctttt cacggtacgg
    18841 atatctggac gctgtatgaa ctttcctggc tgaatgcgaa aggtttgccg caggtcgctg
    18901 tcggtcatgt tgaacttgat tacaccagcg taaatctgat tgagtcgaag agttttaagc
    18961 tctatctcaa cagttttaac cagacgcgtt ttaataactg ggatgaggtg cgccagacgc
    19021 tggagcgcga cttaagcact tgcgctcagg gtaagattag cgtggcgtta tatcgtcttg
    19081 atgaactgga aggccagccg ataggtcatt ttaatggcac ttgcattgat gaccaggata
    19141 tcactatcga taactatgaa ttcactactg actatctgga gaatgccacc tgtggtgaaa
    19201 aagtagtgga agagacgctt gtcagccacc tgctgaaatc aaactgcctg atcacccatc
    19261 aaccagattg gggttcgatc caaattcagt atcgtggacg ccaaattgac agagaaaaac
    19321 tgctgcgtta cctggtctca ttccgtcatc acaacgagtt ccacgaacag tgcgtggaac
    19381 gcatctttaa tgacctgtta cgcttctgcc agccagaaaa attgagcgtt tacgcacgtt
    19441 atacccgtcg tggcggtctg gacattaacc cgtggcgcag taatagcgat tttgtcccat
    19501 cgaccacaag actggttcgg caataaattt tttctcaatt ttgcgtgctg gattcacgca
    19561 gaaggttgtg aaaggacatc aggcagggct attgtaatca aagggaatga cgatattcgt
    19621 cccataagga gtttttcttg attacacata ttagcccgct tggctccatg gatatgttgt
    19681 cgcagctgga agtggatatg cttaaacgca ccgccagcag cgacctctat caactgtttc
    19741 gcaactgttc acttgccgta ctgaactccg gtagtttgac cgataacagc aaagaattgc
    19801 tgtctcgttt tgaaaatttc gatattaacg tcctgcgccg tgaacgcggc gtaaagctgg
    19861 aactgattaa tcccccggaa gaggcttttg tcgatgggcg aattattcgc gcgttgcagg
    19921 ccaacttgtt cgcggttctg cgtgacattc tcttcgttta cgggcaaatc cataacaccg
    19981 ttcgttttcc caacctgaat ctcgacaact ccgtccacat cactaacctg gtcttttcca
    20041 tcttgcgtaa cgctcgcgca ctgcatgtcg gtgaagcgcc aaatatggtg gtctgctggg
    20101 gcggacactc aattaacgaa aacgagtatt tgtatgcccg tcgcgtcggg aatcagctgg
    20161 gcctgcgtga gctgaatatc tgcaccggct gtggtccggg agcgatggaa gcgccgatga
    20221 aaggtgctgc ggtcggacac gcacagcagc gttacaaaga cagtcgtttt attggtatga
    20281 cagagccgtc gattatcgcc gctgaaccgc ctaacccgct ggtcaacgaa ttgatcatca
    20341 tgccagatat cgaaaaacgt ctggaagcgt ttgtccgtat cgctcacggc atcattatct
    20401 tccctggcgg tgtgggtacg gcagaagagt tgctgtattt gctgggaatt ttaatgaacc
    20461 cggccaacaa agatcaggtt ttaccattgt tcctcaccgg cccgaaagag agcgccgact
    20521 acttccgcgt actggacgag tttgtcgtgc atacgttggg tgaaaacgcg cgccgccatt
    20581 accgcatcat cattgatgac gccgctgaag tcgctcgtca gatgaaaaaa tcgatgccgc
    20641 tggtgaaaga aaatcgccgc gacacaggcg atgcctacag ctttaactgg tcaatgcgca
    20701 ttgcgccaga tttgcaaatg ccgtttgagc cgtctcacga gaatatggct aatctgaagc
    20761 tttacccgga tcaacctgtt gaagtgctgg ctgccgatct gcgccgtgcg ttctccggta
    20821 ttgtggcggg taacgtaaaa gaagtcggta ttcgcgccat tgaagagttt ggtccttaca
    20881 aaatcaacgg cgataaagag attatgcgtc gtatggacga cctgctacag ggttttgttg
    20941 cccagcatcg tatgaagttg ccaggctcag cctacatccc ttgctacgaa atctgcacgt
    21001 aatctccctc ccccggtgac gtttgttgcc gggttttctc gtttttggtc acatacccat
    21061 caatttattt catttgttat atgaatgttt cttaccaccc tcacgcgata aatatcattg
    21121 cagttaatat gtcatacaat ttatgttgta acgcaaacgt ttccctattt tcataaacca
    21181 cattttatcg ctatgaaaaa gaaattatcg ccatgattaa ctaaaagtat tgattttttc
    21241 agttcaacct acatatattg cgcgccccgg aagaagtcag atgtcgttta atgggcaaat
    21301 attgccctta aattctcttt tacttttgat ttacagagta aagcgttggg ataatctatc
    21361 ttccaagtag attattgtat ttgggatcaa gatcactgat agatacataa cttgtgtgta
    21421 tctttccgcc ctcaaattat tacggcggta aatgattaag ccatcgccga tagacagatt
    21481 tcatttttac ggtcaggcac cttcccgagc tgaactggct aaaagctgaa ttatttgcat
    21541 tcctccagga gaaatagatg gaaacgactc aaaccagcac gattgcgtcg aaagactctc
    21601 gtagtgcctg gcgcaagaca gacaccatgt ggatgctggg cctttacggc acggcaatcg
    21661 gcgcgggcgt gctgttcctg ccaatcaacg ccggtgttgg cggtatgatc ccgctgatca
    21721 tcatggctat ccttgcgttc ccgatgacat tttttgctca ccgcggcctg actcgcttcg
    21781 tactgtctgg taaaaaccca ggcgaagaca tcaccgaggt tgtagaagaa cactttggta
    21841 ttggcgcagg taaactgatt accctgctct acttcttcgc tatctacccg atcctgctgg
    21901 tttatagcgt ggcaatcacc aatacagttg aaagcttcat gtctcaccag ctgggtatga
    21961 caccaccgcc gcgtgcgatt ctgtcgctga tcctgatcgt gggtatgatg accatcgttc
    22021 gcttcggtga gcagatgatc gttaaagcaa tgagtattct ggtattcccg tttgttggcg
    22081 tactgatgct gctggctctg tacctgatcc cgcagtggaa cggcgcagca ctggaaacgc
    22141 tgtctctgga cactgcatct gcaaccggaa acggtctgtg gatgaccctg tggctggcaa
    22201 ttccggttat ggtgttctct ttcaaccact ctccgatcat ctcttctttc gccgttgcga
    22261 agcgtgaaga gtacggcgat atggcagaac agaaatgctc gaagatcctg gcattcgcac
    22321 acatcatgat ggtgctgacc gtaatgttct tcgtcttcag ctgtgtactg agcctgactc
    22381 cggcagacct ggctgcggct aaagagcaga acatctcgat tctgtcttac ctggctaacc
    22441 actttaacgc accggttatc gcgtggatgg ctccgattat cgcgattatc gctatcacca
    22501 aatccttcct cggccactac ctgggcgcac gtgaaggctt caacggtatg gtgattaaat
    22561 ctctgcgtgg taaaggtaag tctatcgaaa tcaacaagct gaaccgtatc actgcgctgt
    22621 tcatgctggt aacgacctgg attgttgcca ccctgaaccc gagcatcctg ggtatgattg
    22681 aaaccctggg cggcccaatc atcgcgatga tcctgttcct gatgccgatg tacgcaattc
    22741 agaaagtacc ggcaatgcgt aagtacagcg gtcacatcag caacgtattc gttgtcgtga
    22801 tgggtctgat tgcaatctcc gcaatcttct actctctgtt cagctaagtc ctttcgcgcc
    22861 gctttcgggc ggcgcttcct ccgttttaac acgatgtatt tcctatgatt agcgtattcg
    22921 atattttcaa aatcggcatt ggcccttcca gttctcatac cgttggacca atgaaagcgg
    22981 gtaaacaatt taccgacgat ctgattgccc gtaatctgct taaagacgtg acccgcgtgg
    23041 tggttgacgt gtacggctcg ctctctctga ccggtaaagg ccaccacact gatatcgcca
    23101 ttattatggg cctggcgggt aacctgccgg ataccgtgga tatcgattcc atccctggtt
    23161 ttattcagga tgtgaatact catggtcgcc tgatgctggc aaacggtcag catgaagtgg
    23221 agttcccggt tgatcagtgc atgaacttcc atgcagacaa cctttctctg catgaaaacg
    23281 gtatgcgcat taccgcgctg gcgggcgata aagtcgttta cagccagact tactactcta
    23341 ttggcggtgg ctttatcgtt gatgaagagc attttggcca gcagaatagc gcaccggttg
    23401 aagttcctta tccgtacagt tcagcagccg atctgcaaaa acattgtcag gaaaccgggc
    23461 tgtcactctc tggcctgatg atgaaaaatg agctggcgct gcacagcaaa gaagagctgg
    23521 aacagcacct ggcgaacgta tgggaagtca tgcgtggcgg tattgagcgc ggtatttcca
    23581 ccgaaggcgt gttgcctggc aaactgcgcg ttccacgccg tgctgcggca ctacgccgga
    23641 tgctggtcag ccaggataaa accaccactg acccgatggc ggttgttgac tggatcaaca
    23701 tgtttgcact ggcagtgaac gaagagaacg ctgctggcgg acgcgtggtg actgcgccga
    23761 ctaacggtgc gtgcgggatt atcccggcag tgctggcgta ctacgacaag ttcatccgcg
    23821 aagtgaacgc taactcactg gctcgttacc tgctggtagc cagtgccatt ggttctcttt
    23881 ataagatgaa cgcgtcaatt tctggtgctg aagtcggctg ccagggtgaa gttggtgtgg
    23941 cgtgctcaat ggcggcggct ggtctggcag aactgttagg tgcaagcccg gcgcaggtgt
    24001 gcatcgcggc ggaaatcgcc atggagcaca acctcggtct gacgtgtgac ccggtcgccg
    24061 gacaggtcca ggtgccatgc atcgagcgta acgccattgc ggcagtaaaa gcggtgaacg
    24121 cctcacgtat ggcgctacgc cgtaccagcg agccgcgcgt ctgcctcgat aaagttatcg
    24181 aaaccatgta cgaaacaggt aaagatatga acgccaagta ccgcgaaacc tctcgcggcg
    24241 gcctggcaat gaagatcgtt gcctgcgatt aatcgctctc cagaggcctc gttttgcgag
    24301 gcctcttccc gatttctcat ccagccgtag cctgttccgg catcgaatgt tacccttatc
    24361 gcctgatctt taagggggtt atcgtggctg ttcatttgct tattgtcgat gcactgaatc
    24421 ttattcgtcg cattcatgcc gttcagggat cgccctgtgt cgaaacctgc cagcatgcgc
    24481 tcgatcagct cattatgcac agccagccaa cccacgcggt cgccgttttt gatgatgaaa
    24541 accgcagtag cggctggcgt catcagcgtt taccagatta caaagcgggt cgaccgccaa
    24601 tgccggaaga gttgcacgac gagatgcctg cattacgcgc cgcctttgag caacgcggcg
    24661 tcccgtgctg gtcaaccagc ggcaacgaag ccgatgactt agccgccacg ctggcggtca
    24721 aagtgacaca ggccgggcat caggcaacga ttgtttcgac agataaaggc tactgtcagt
    24781 tactttcacc gacattacgt attcgtgatt acttccagaa acgttggctg gatgcgccat
    24841 ttatcgataa agaatttggc gttcaaccgc agcagttgcc cgattactgg ggacttgcgg
    24901 ggatcagcag ttcaaaggta ccgggtgttg cgggaatcgg accaaaaagc gccacgcagc
    24961 tgctggtcga gtttcagagt ctggaaggga tatatgagaa tctggatgcg gttgccgaaa
    25021 agtggcgcaa aaaattagaa acccataaag agatggcgtt tctgtgccgc gatattgccc
    25081 gcttacaaac cgatttgcat atcgacggca atttacagca attgcggttg gtacggtaac
    25141 ggcgagccgg atgcaaacga ttatcaacac attgaattta atgcacttat ataccggata
    25201 agacgcggct agcgtcgtat ccggcattat cacatcagcg catttaccag gcggtatggt
    25261 aaagctctac aatatcctca agcgttgctt cacgcgggtt gccaccggta caaacatcat
    25321 ccagtgccgc ctgcgccagt gccggaatgt cttccttgcg tacaccaaca tcacgcaaat
    25381 gtggcggaat accgacatca cggttgagag caaacaccgc ttcaacagcg gcattacgcg
    25441 cctcttccag gctcatacct tccactttca cgcccataac gcgcgcgata tcgcggtact
    25501 tctcaccggt aaagtcagcg ttatagtgca tgacatgcgg taacaggatg gcgttcgcaa
    25561 caccgtgtgg agtgttgtaa aacgcgccca gtggatgcgc cataccatgc accaacccta
    25621 acccaacatt cgagaagccc atacccgcaa catactgccc gagcgccatt tcttctccgg
    25681 catccttatc accagcaacc gatcctcgca gcgccccagc aatgatttca atcgctttaa
    25741 tgtgcagtgc atcggttagc gcccacgcgc cacgggtaat atacccctca atagcatgag
    25801 tgagcgcatc gacacccgtc gcagctttca gcgctggagg cataccatcc atcatgtcag
    25861 cgtcaataaa cgccacctgc gggatatcat gcggatcaac gcaaacaaac ttgcgccgtt
    25921 tctcttcgtc agtgatcacg tagttaatgg tcacttctgc cgcagtacct gctgtggtag
    25981 gaattgccag aatcggtaca ctgggtttat tggtcgggga aagcccttcc aggctacgca
    26041 catcggcaaa ctccggattg ttgctgataa tgccaatcgc tttacaagta tcctgtggag
    26101 aaccaccacc aatagcgatc aggtaatccg cgccgctatt ctggaataca ccgagccctt
    26161 ctttgacgac agtaattgtt gggttgggca ctacgccgtc gtaaatcgcc catgccagcc
    26221 ctgcagcatc catcttatcg gtcactttcg ccaccacgcc gcattgcacc agcgttttat
    26281 cggtgacgat cagcgccttc tgataaccac ggcgtttcac ctcatcggtt aaagccccaa
    26341 cagcaccccg accaaaccat gccgtttcgt tcagaatcat tctgttagcc atcatccttc
    26401 tccttgttgc tttacgaaat tactcttcaa tccgtaaccc ataggttttg aatttctcca
    26461 gcactacggc aatctcttca tcgctcagca ctggcaccgg gtccgtaatc gccagggtcg
    26521 ccacgtaaag ttgcgccagc acttcaactt catgcgccag ccataacgct ttttccagat
    26581 tcacctcaca agcgataagc ccatgatgtt gtaacaaagt tgccttacga tttttgagag
    26641 ccagcgcaac atgttcagaa agttcgcgtg ttccaaaggt cgcataaggc gcgcaaggaa
    26701 tagaattacc acctgccgcc gcaatcatgt agtgaatagc ggggatcggt cggttaagaa
    26761 tggaaactgc tgtgcaatga acggcatgat tgtgaacaac cgcgttggca tccggtctgc
    26821 tttgataggc cgccatatgg aaacgccatt cgcttgaggg gagctttcct tcctcatgtt
    26881 taccgttgcc atcaataaag acaatatgcg actccgtcag tttttcatat ggaatgcctg
    26941 taggcgtaat cagcatccca tcctgataac gtacactgac gttccccgct gtcccctggt
    27001 tcagtcccag gcgggtcatt tccaggcaag tgtcaataat ctgacgagca agtttatttc
    27061 gttccattag ctacctctct ctgattcaaa acagggcaat aatgttgttc ctttcacact
    27121 attgaattag ccgtttaatt acccaccatc ttcttcctga ttaacaagaa agaaattcac
    27181 aagcttatat tttgtgacct agctcaacta atcacagtaa ataactgcaa gttctctttt
    27241 tataacccca ttaaaaatga ccgctcttaa aaatatttat caaaacggtc atttttctat
    27301 tcacccaagc caggaatgcc cgttttcggc ataaacaatt aatacagtca tctggtttgt
    27361 gttttttatg atttattttc tgaaacgggc atgaaatttc gattattaaa gtgatggtag
    27421 tcacataaag tcaccttcca gctaataagt gtgaccgccg tcatattaca gagcgttttt
    27481 tatttgaaaa tgaatccatg agttcatttc agacaggcaa atattcactg atatgaagcc
    27541 cgaactcgct ggttttgcac ttttgaaaac ataaccgatt acgtgcttaa gcttctgaac
    27601 ctaagaggat gctatgggaa acacatcaat acaaacgcag agttaccgtg cggtagataa
    27661 agatgcaggg caaagcagaa gttacattat tccattcgcg ctgctgtgct cactgttttt
    27721 tctttgggcg gtagccaata accttaacga cattttatta cctcaattcc agcaggcttt
    27781 tacgctgaca aatttccagg ctggcctgat ccaatcggcc ttttactttg gttatttcat
    27841 tatcccaatt cctgctggga tattgatgaa aaaactcagt tataaagcag gaattattac
    27901 tgggttattt ttatatgccc tgggcgctgc attattctgg cccgctgcag aaataatgaa
    27961 ctacacctta tttttagttg gcctatttat tattgcagcc ggattaggtt gtctggaaac
    28021 tgccgcaaac ccttttgtta cggtattagg gccggaaagc agtggtcact tccgcttaaa
    28081 tcttgcgcaa acatttaact cgtttggcgc aattatcgcg gttgtctttg ggcaaagact
    28141 tattttgtct aacgtgccac atcaatcgca agacgttctc gataaaatgt ctccagagca
    28201 attgagtgcg tataaacaca gcctggtatt atcggtacag acaccttata tgatcatcgt
    28261 ggctatcgtg ttactggtcg ccctgctgat catgctgacg aaattcccgg cattgcagag
    28321 tgataatcac agtgacgcca aacaaggatc gttctccgca tcgctttctc gcctagcgcg
    28381 tattcgccac tggcgctggg cagtattagc gcaattctgc tacgttggcg cacaaacggc
    28441 ctgctggagc tatttgattc gctacgctgt agaagaaatt ccaggtatga ctgcaggctt
    28501 tgccgctaac tatttaaccg gaaccatggt gtgcttcttt attggtcgtt tcaccggtac
    28561 ctggctcatc agtcgcttcg caccacacaa agtcctggcc gcctacgcat taatcgctat
    28621 ggcactgtgc ctgatctcag ccttcgctgg cggtcatgtg ggcttaatag ccctgacttt
    28681 atgcagcgcc tttatgtcga ttcagtaccc aacaatcttc tcgctgggca ttaagaatct
    28741 cggccaggac accaaatacg gttcgtcctt catcgttatg accattattg gcggcggtat
    28801 tgtcactccg gtcatgggtt ttgtcagtga cgcggcgggc aacatcccca ctgctgaact
    28861 gatccccaca ctctgcttcg cggtcatctt tatctttgcc cgtttccgct ctcaaacggc
    28921 aactaactga acatattttc cgaataaagt gaggaatctg taatgaaaaa aatcagctta
    28981 ccgaaaattg gtatccgccc ggttattgac ggtcgtcgca tgggtgttcg tgagtcgctt
    29041 gaagaacaaa caatgaatat ggcgaaagct acggccgcac tgctgaccga gaaactgcgc
    29101 catgcctgcg gagctgccgt cgagtgtgtc atttccgata cctgtatcgc gggtatggct
    29161 gaagccgctg cttgcgaaga aaaattcagc agctagaatg taggcctcac cattacggta
    29221 acgccttgct ggtgctatgg cagtgaaacc atcgacatgg atccaacccg cccgaaagcc
    29281 atttggggct ttaacggcac tgaacgtccc ggcgctgttt acctggcagc ggctctggca
    29341 gctcacagcc agaaaggcat cccggcattc tccatttacg gtcatgacgt tcaggatgcc
    29401 gatgacacat cgattcctgc cgatgttgaa gaaaaactgc tgcgctttgc ccgcgccggt
    29461 ttggccgtcg ccagcatgaa aggtaaaagc tatctgtcgc tgggcggcgt ttcgatgggt
    29521 atcgccggtt ctattgttga tcacaacttc tttgaatcct ggctgggaat gaaagtccag
    29581 gcggtggata tgaccgaact gcgtcgccgt atcgatcaga agatttacga cgaagccgaa
    29641 ctggaaatgg cactgacctg ggctgataaa aacttccgct atggcgaaga tgaaaataac
    29701 aaacagtatc aacgcaatgc cgagcaaagc cgcgcagttc tgcgcgaaag tttactgatg
    29761 gcgatgtgta tccgcgacat gatgcaaggc aacagcaaac tggccgatat tggtcgcgtg
    29821 gaagaatcac ttggctacaa cgccatcgct gcgggcttcc aggggcaacg tcactggacc
    29881 gatcaatatc ccaatggtga caccgccgaa gcgatcctca acagttcatt tgactggaat
    29941 ggcgtgcgcg aaccctttgt cgtggcgacc gaaaacgaca gtcttaacgg cgtggcaatg
    30001 ctaatgggtc accagctcac cggcaccgct caggtatttg ccgatgtgcg tacctactgg
    30061 tcaccagaag caattgagcg tgtaacgggg cataaactcg atggactggc agaacacggc
    30121 atcatccatt tgatcaactc aggttctgct gcgctggacg gttcctgtaa acaacgcgac
    30181 agcgaaggta acccgacgat gaagccacac tgggaaatct ctcagcaaga ggctgacgct
    30241 tgcctcgccg ctaccgaatg gtgcccggcg atccacgaat acttccgtgg cggcggttac
    30301 tcttcccgct tccttaccga aggcggcgtc ccgttcacca tgactcgtgt caacatcatc
    30361 aaaggcctgg gatcggtact gcaaatcgcg gaaggctgga gcgtggaatt gccgaaggat
    30421 gtgcatgaca tcctcaacaa acgcaccaac tcaacctggc caaccacctg gtttgcaccg
    30481 cgcctcaccg gtaaagggcc gtttacggat gtgtactcgg taatggcgaa ctggggcgct
    30541 aaccatgggg ttctgaccat cggccacgtt ggcgcagact ttatcactct cgcctccatg
    30601 ctgcgtatcc cggtatgtat gcacaacgtt gaagagacca aagtgtatcg tccttctgcc
    30661 tgggctgcgc acggcatgga tattgaaggc caggattacc gcgcttgcca gaactacggt
    30721 ccgttgtaca agcgttaata cattactccc tgatgtgatg cccggtcgct ccggctaccg
    30781 tgcctgaaca agcaagagtg gttagccgga taagcaatgt tatccggcta tattgcagga
    30841 gcgattatga aacaagaagt tatcctggta ctcgactgtg gcgcgaccaa tgtcagggcc
    30901 atcgcggtta atcggcaggg aaaaattgtt gcccgcgcct caacgcctaa tgccagcgat
    30961 atcgcgatgg aaaacaacac ctggcaccag tggtctttag acgccatttt gcaacgcttt
    31021 gctgattgct gtcggcaaat caatagtgaa ctgactgaat gccacatccg cggtatcgcc
    31081 gtcaccacct ttggtgtgga tggcgctctg gtagataagc aaggcaatct gctctatccg
    31141 atcattagct ggaaatgtcc gcgaacagca gcggttatgg acaatattga acagttgatc
    31201 tccgcacagc ggttgcaggc tatttctggc gtcggagcct ttagtttcaa tacgttatat
    31261 aagttggtgt ggttgaaaga aaatcatcca caactgctgg aacgcgcgca cgcctggctc
    31321 tttatttcgt cgctgattaa ccaccgttta accggcgaat tcactactga tatcacgatg
    31381 gccggaacca gccagatgct ggatatccag caacgcgatt tcagtccgca aattttacaa
    31441 gccaccggta ttccacgccg actcttccct cgtctggtgg aagcgggtga acagattggt
    31501 acgctacaga acagcgccgc agcaatgctc ggcttacccg ttggcatacc ggtgatttcc
    31561 gcaggtcacg atacccagtt cgcccttttt ggcgctggtg ccgaccaaaa tgaacccgtg
    31621 ctctcttccg gtacatggga aattttaatg gtccgtagcg cccaggttga tacttcgctg
    31681 ttaagtcagt acgccgattc tacctgcgaa ctggatagcc aggcagggtt gtataaccca
    31741 ggtatgcaat ggctggcatc cggcgtgctg gaatgggtga gaaaactgtt ctggacggct
    31801 gaaacaccct ggcaaatgtt gattgaagaa gctcgtctga tcgcacctgg cgcggatggt
    31861 gtgaaaatgc agtgtgattt attgtcgtgt cagaacgctg gctggcaagg agtgacgctt
    31921 aataccacgc gggggcattt ctatcgcgcg gcgctggaag ggttaactgc gcaattacag
    31981 cgcaatctac agatgctgga aaaaatcggg cactttaaag cctctgaatt attgttagtc
    32041 ggtggaggaa gtcgcaacac attgtggaat cagattaaag ccaatatgct tgatattccg
    32101 gtaaaagttc tcgacgatgc cgaaacgacc gtcgcaggag ctgcgctgtt cggttggtat
    32161 ggcgtagggg aatttaacag cccggaagaa gcccgtgcac agattcatta tcagtaccgt
    32221 tatttctacc cgcaaactga acctgaattt atagaggaag tgtgaaatgc tgaaaacaat
    32281 ttcgccgtta atttctcccg aactattgaa agtgctggca gagatgggac atggagatga
    32341 aattattttt tccgatgctc actttcccgc ccattcgatg ggaccgcagg tgatccgcgc
    32401 tgatggcctg ttggtgagcg acttgctcca agcgattatc ccattatttg agctggacag
    32461 ttatgccccg ccgctggtga tgatggcagc ggtagaaggt gacactctcg atcctgaagt
    32521 agaacgacgt taccgtaatg cgctttcact acaagccccg tgtcctgaca tcatccgcat
    32581 caatcgtttt gcgttttatg aacgggcgca aaaagccttt gcgatcgtta tcacaggcga
    32641 acgagcgaag tacgggaata ttcttttaaa aaaaggggta acaccgtaat ctcatatcgg
    32701 tacgcccgca tgacgcgggc ggttatcgaa tgatggggtg aaaaatatga aagcggcacg
    32761 ccagcaagcg atagtcgacc tgctgctgaa ccataccagc ctgaccacgg aagctctctc
    32821 tgaacagcta aaggtcagta aagaaaccat tcgtcgcgat ctcaatgaat tacagacgca
    32881 gggtaaaatt ctgcgcaatc atggacgcgc taaatatatc caccgtcaaa atcaagacag
    32941 tggcgatccc tttcacatca ggctgaaaag ccattatgcg cataaagcag atatcgcgcg
    33001 cgaggcgctc gcgtggattg aagaagggat ggtgatagcc ttagacgcca gttcaacttg
    33061 ctggtatctg gcacgccagt tgcctgacat caacattcag gtcttcacca atagccatcc
    33121 gatttgccat gaactcggta aacgcgaacg cattcaactg atcagttccg gcggcacact
    33181 tgagcgcaaa tatggctgtt acgtcaatcc ctcgctgatt tcccaactta aatcgctgga
    33241 aatcgatctg tttatttttt cttgtgaagg gatcgatagc agcggcgcac tgtgggactc
    33301 caatgcgatc aacgctgatt acaaatcgat gctattaaaa cgtgccgcgc aatcgttgtt
    33361 attgattgat aaaagtaaat ttaatcgttc aggggaagcc cgcatcgggc atctggatga
    33421 ggtaacgcac attatttctg atgagcgcca ggttgcaact tctttggtaa cagcctgacg
    33481 gaaagggtag caggccgaag acgacgcccc ggccttgcct gttatcgctc gtcgcgacga
    33541 ccacccaccg cagcccagat gcggcggacg tgcaccgtca cttcttcgcg atcgtgatac
    33601 aactgccgcg cctgaatctg agcatttatg ccatgttcat caagctgtgc ctgaatatac
    33661 gccagattgt gtgatacttc ttcgtagcgt tttttcatcg gcagtttgag attgaaaatg
    33721 gtttcacggc accagccatt aaccagccac tgcgccatca atgccgcaac tttagccggt
    33781 ttttcaacca tatcgcatac catccaggag atattgctgc gtgtcggacg gaatttgaaa
    33841 ccgtcttccc gcagccacgt cacctgcccg gtatccatca gactttgcgc catcgggccg
    33901 ttgtcgacgg aataaaccca catgttgcgc ttcaccagtt ggtaggtcca gccgccaggg
    33961 caagcgccta aatccaccgc ccacatcccg ttcgccaggc gttcatccca ctcatccgca
    34021 ggaataaaca catgaaatgc ctcttccagt ttgagcgtgg aacgactcgg cgcctctgcc
    34081 ggaaatttca ggcgcggaat gcccatatag aacggcgaat tattgttgct gtatgagtaa
    34141 ccggtatagc agcagcctgg tgcaataaag aatacatgca caaccggacg cttcggcgtt
    34201 tcatagttcg ccagcacccc ggcatcgcgc aaggcagcgc gtagcggcac ggtgaattta
    34261 cggcagaatt tcagcaactc tttgctttcg ttggtatcgg caacttcaac acgcagttca
    34321 ccgcccttct ctactacgcc ctgcaacatg tcgacaatgg gggtaatacg atcttctggc
    34381 ggcaaatgct gcaagagttc ccccaccaca aaccactggc gggcaaaaat taatgaactg
    34441 aacggcagct cacggattaa cttatcgcca tcatcaggtt gataacattc ataaatgaca
    34501 taacccgcat tctctttcac gcgggcaaaa ccgaaaattt cccgctggcc ggctttatcg
    34561 gtaatttctg cggcgcactc tttttcaaag cccggacggc acagcaatac aaccttattc
    34621 atgacttacg cccttgcgct ttaaacggat agcaccaact aacattaacg cccagcccgc
    34681 gaggaagctc acgccgccaa ccggtgtgac aaatgcccac aaacgcagat gggacagcgc
    34741 caggcaataa aggctgccgc tgaacaacac cgtgcctaac gcgaggaaaa cgctactcca
    34801 gtaaaaccag atactgatgc gacgctgcat tgccaccgcc agacctaaga tcgccagcgt
    34861 atgaaacgcc tggtattcga ggccggtctg gatccagccc atctcaacgg cccccatggt
    34921 tttacttaac acatgcgcgc caaaagcgcc cagagccaca aaaatgaagc cgctaatggc
    34981 ggcgaaaatc agcataaaac ggctggtcat ggtcgtaccc tacgtaaatt attgttcata
    35041 acgaaagcgg aatttttctt gttcagcagc ggctttcgcc aggatccatt ggcgaaaggc
    35101 ggctatttta cccagttctg cctggctgtc atgacaaacc agataaaaag cgtttttact
    35161 gaccagaaca tcattaaacg ggcaaacaag acgtccggcc tcgatttcag attgcgccat
    35221 cacgttattt gccagcgcca ctccctgccc gtgaatagcc gcttgcagca ccatggcgct
    35281 atgactaaaa attggccctt gctgaacgtt gatatgattt aaccccaact gtcgggtata
    35341 tgtctgccag tcacggcgtg acgcatcatg taataacgta tgtttagcca gatcttccgg
    35401 ggtcttcaag ggtttttcgc ctgtcagcag tagcggcgaa cacaccggca ataaatattc
    35461 ggcgtacagt ttttccaccc gtagccccgg ccagttgccc cgaccataaa atatcgccac
    35521 atcaacatca tccgccagct tatcttcctg acgatcaacc gcctggattc gaacgtcaat
    35581 tcccggataa gctgaattaa agctggaaag tcgcggaacc aaccaatgaa tggcgaaact
    35641 ggggagtaaa ctgaccgtca acgccccctt ggcgctacgg gcctggagtt tacgcgtcgc
    35701 ttcggttaat tgcgaaaata tctctttgat atcgaggaaa tagctttgcc cttcctcggt
    35761 cagcaggagt gaacgattac ggcggcggaa cagttttagc cccaaaaaat cctcaagaga
    35821 cttgatttga tgacttactg cggcctgggt cacgaaaagc tcttctgctg cgcgagtgaa
    35881 acttaaatgg cgtgctgcgg catcaaaaac tcgtaaggca tttagcggtg gtaatcgttt
    35941 agacatggct tttaaacttt gatgttaaat taatttaaca attagatcac actgtgtaac
    36001 ctattagttt ttttaatctg agccattata aattgtccgt tgagcttcta ccagcaaata
    36061 cctatagtgg cggcacttcc tgagccggaa cgaaaagttt tatcggaatg cgtgttctgg
    36121 tgaacttttg gcttacggtt gtgatgttgt gttgttgtgt ttgcaattgg tctgcgattc
    36181 agaccatggt agcaaagcta ccttttttca cttcctgtac atttaccctg tctgtccata
    36241 gtgattaatg tagcaccgcc taattgcggt gctttttttt accttgcgat cgcgaattac
    36301 tgatccagtt cgaccatctc tttcacgtca gtacggttga tctgctgttt gttgccatta
    36361 gcgtctttat acgaaatcat accggtatcg ttatcagtct gtggtttgcc gtcagagacg
    36421 atggtacgtc cgtcattggt gtgcatcaca tagttcgaac cggaacaggc gctcagggca
    36481 aaagtcagca tacaggcaga aataattgcg gcagtctttt tcatcatctt ctccttaaag
    36541 cgattaataa atcttaatag tattgattaa caggctaaaa ttaacgccta acactattca
    36601 gcatatgtta cttacgcggc ttcgccagga tatccagata attctgatgg ttagcactct
    36661 ccttgtatca aagtgaattt tgcgtcacga tcggtgcatc aagccgagga gtaccatgaa
    36721 cgtttttaat cccgcgcagt ttcgcgccca gtttcccgca ctacaggatg cgggcgtcta
    36781 tctcgatagc gccgcgaccg cgcttaaacc tgaagccgtg gttgaagcca ctcgacagtt
    36841 ttacagcctg agcgccggaa acgtccatcg cagccagttt gccgaagccc aacgcctgac
    36901 cgcgcgttat gaagctgcac gagagaaagt ggcgcaatta ctgaatgcac cggatgataa
    36961 aactatcgtc tggacgcgcg gcaccactga atccatcaac atggtggcac aatgctatgc
    37021 gcgtccgcgt ctgcaaccgg gcgatgagat tattgtcagc gtggcagaac accacgctaa
    37081 cctcgtcccc tggctgatgg tcgcccaaca aactggagcc aaagtggtga aattgccgct
    37141 taatgcgcag cgactgccgg atgtcgattt gttgccagaa ctgattactc cccgtagtcg
    37201 gattctggcg ttgggtcaga tgtcgaacgt tactggcggt tgcccggatc tggcgcgagc
    37261 gattaccttt gctcattcag ccgggatggt ggtgatggtt gatggtgctc agggggcggt
    37321 gcatttcccc gcagatgttc agcaactaga tatcgatttc tatgctttct ctggtcacaa
    37381 actgtatggc ccgacgggta tcggcgtgct gtatggcaaa tcagaactgc tggaagcgat
    37441 gtcgccctgg ctgggtggcg gcaaaatgat tcacgaagtg agttttgacg gcttcacgac
    37501 tcaatctgcg ccgtggaaac tggaagcagg aacgccaaat gtggctggtg tcataggatt
    37561 aagcgcggcg ctggaatggc tggcagatta cgatatcaac caggcagaaa gttggagtcg
    37621 cagcctggcg acgctggcgg aagatgcgct ggcaaaacgt cctggcttcc gctcattccg
    37681 ctgccaggat tccagcctgc tggcctttga ttttgctggc gttcatcaca gcgatatggt
    37741 gacgctgctg gcggagtacg gtattgccct gcgggccggg caacattgcg ctcagccgct
    37801 actggcagaa ttaggcgtaa ccggcacact gcgcgcctct tttgcgccat ataatacaaa
    37861 gagtgatgtg gatgcgctgg tgaatgccgt tgaccgcgcg ctggaattat tggtggatta
    37921 atgacgaacc cgcaattcgc cggacatccg ttcggcacaa ccgtaaccgc agaaacgtta
    37981 cgcaatacct tcgcaccgtt gtcgcaatgg gaagataaat atcgccagtt gatcatgctg
    38041 gggaaacagc ttccggcttt gccagacgag ttaaaagcgc aggctaaaga gattgccgga
    38101 tgcgaaaacc gcgtctggct gggatatacc gtgactgaaa acggtaaaat gcacttcttt
    38161 ggcgatagcg aagggcgcat tgtgcgcggc ctgctggcgg tgctgttgac tgccgttgag
    38221 gggaaaaccg ccgccgaatt gcaggcacag tcgccgctgg cattgtttga tgagctggga
    38281 ttgcgtgcgc aacttagcgc ctcacgcagc caggggttaa atgcgttaag cgaggcgatt
    38341 atcgctgcga cgaagcaggt ttaagcatag aaccgggtcg gataagacgt tcgcgtctca
    38401 tccgacctga ttgtttaacc ctgacgcgcc gctttcgcca tcatcttctt cagcgcatga
    38461 gaaaccgcaa caaacccaaa ggtggcggtc accatcgttg ccgcgccaaa tccagatgca
    38521 caatccatcc gcttcggccc ttctgccgtg gctttcatcg cacacacagt accgtctgac
    38581 tgcgggtaca ccagcgcttc agtagaaaac acgcaatcga cgccgagctt acctttactg
    38641 tttttcacta cgccaaaatc gcttttcagg cgctcgcgca acttcgccgc cagcggatcc
    38701 tgaatcgttt tcgccagatc ggtaacctga atctgcgtcg ggtcaatctg cccacccgcg
    38761 ccaccggtcg tgaccagcgg gattttattg cgccgacaat aagcaatcag cgccgctttg
    38821 ggccgtacac tatcaatggc atcaatcacg tacgaataac ccacgctcat atactgcgct
    38881 acgttatccg gcgtcacgaa atcatccacc accgttacac ggcactccgg gttaatctgg
    38941 cgaatacgct ccgccatgac ttccgctttt gccagcccga cgttatcgcg cagagcgtga
    39001 atttgccgat tggtattggt gacgcacaca tcatccatat cgataagcgt gattgcgcca
    39061 atccccgtgc gcgccagcgc ttccgccgcc caggaaccga caccaccgat accaaccaca
    39121 caaatatgcg cgtcagcaaa cagttgcaac gctttttcac catacagacg cgctgtgcca
    39181 ccaaaacgct gacgccatgc atcactaatt accacagaca tagcacctca gaattaaaaa
    39241 gggtgaggtt atccctcacc ctgtcgtatc cgtaaaaacg gtatacagaa taacacatca
    39301 gccgctaaag acgttacctg cgcccggggc ggttttcagc acccagacac gtccatagtg
    39361 gttgtaccaa cctgcgcggt gtccggcttc cggcccgatc ccttgataga tatcgaagtg
    39421 ttggcctttg attgcaccac cgacatccag cgccaccatc agacgcagtt cgtactgacc
    39481 attaaatttg ccgttattat ccagcaacgg cacttctgcc agcaaggtag tacctggcgg
    39541 aataatggaa cgatcagagg caactgacgc gcgaccaacc agcggcaccg cacttgcccc
    39601 tttcaccggt gcaaaagatt gcggtttaaa gaagacgaaa gacgggttct gttccagcag
    39661 ctcgcgaacc tcggcttcac tgtgtgtttc gccccagtga cgaatcgcct gcatcgacat
    39721 atcttctttt ttcacttcgc cacggtcgat cagcacctta ccaatgctgc gataggcatg
    39781 accgtttttc cctgcatagc tgaaaaagtt aagcggactg ccatcaccaa agtcgatata
    39841 cccactaccc tgcacatcca taatgaagtt atccatcagg gagttactgt aagcgagaat
    39901 atatttatca ctcaatgccc ccgcgtagat ctccgcacga gacggcagac gaccacgttt
    39961 tggcggcata cggtaaatag gatactggaa ctcgccctgg cgggtatggc gcgcctgaat
    40021 taccggcgtg taatagcctg taaactgcac gttaccatag ttgtcggcac cttccatctg
    40081 ccaggcatca atgccgaact ggcgcatatt gcgggtatca ccgcctgcgc gcagccactc
    40141 ttgcaccgcg ttataaacat tactctggtt gccatacaga cgcggtgacg aattacggat
    40201 atggttaatt tgctcggcaa aatcaccggc gttaatcggc gcgccaacgg catctggctg
    40261 gttcaccaga gagaaaggct gggtaaattt cccgtcttta tattgctgtc cgcgatcggt
    40321 tggtttggaa gagcaggcgg caagcattgc cacaaccgtg cccataagaa ggtactttac
    40381 ccaacgtcct ttcattgttc tcttcttcag gttaaaaaat aaggcgcaac gaagataaca
    40441 aaccgccgcg tttaatgaaa tgagcagcct ctccctgacg caaattttgc acaaaaaata
    40501 ggctttagtg atttgttttt gttcaaaatc atgccagatc cgtgatcggg gtaaaaaaaa
    40561 ggttgcatga aaacgcgagc ggagtatagt gcgcatccac ggaggcgggg tggagcagcc
    40621 tggtagctcg tcgggctcat aacccgaagg tcgtcggttc aaatccggcc cccgcaacca
    40681 attaaaattt gatgaagtaa agcagtacgg tgacgcgggg tggagcagcc tggtagctcg
    40741 tcgggctcat aacccgaagg tcgtcggttc aaatccggcc cccgcaacca attattgaac
    40801 accctaacgg gtgttttttt gtttctactc tcccataaaa aaagcgccat tcagcgcctt
    40861 tttatcatcc ccttctcgcc agcgttgccc catcggcaaa atacgcttta atccccgcaa
    40921 gaatagactc cgcaacttcc tgctggaaag tcgccgtttt cagtttacgc tcttcctcaa
    40981 cgttactgat aaacgccgtt tcgaccagaa tggagggaat atctggtgcc tttagtacgg
    41041 caaacccggc ctgttcaact tgatttttat gcagcttgtt gattttaccg agcttattca
    41101 gcactgcttt accaaactta aggctgtcag caatggtcag cgactgcacc atatcgaaca
    41161 tggtgtggtc gacatagcgg tcaccgcttt tgctcacgcc accaatcaag tccgaggcgt
    41221 tctgggtttg tgccagatat tttgccgcag tactggttgc acctttggtt gagagcgcaa
    41281 acaccgagga accgctcggc tggcgactgg taaaggcgtc ggcatggata gagacaaaca
    41341 ggtcagcacg ctgtttctgg gcttttgcta cgcgcacttg caacggaatg aagatgtctt
    41401 cattgcgcgt catgtacacc ttcatattgc cctctttctc gatcagagag cgcaaacggc
    41461 gagctatttg caataccaca tctttttcgc gcgttttgta tttccccacc gcaccggagt
    41521 cttcgccacc gtggccaggg tcaagcataa tgacaatcgg acgatcgcgc cctgctttac
    41581 ctggttgtgg accactttgc gctggcggca cctgcttttc gaggtcgcct ttgttgtaat
    41641 cctccagcag cgccagcagc gggtcctgca tatcctgtgc attggccgga tagaggtcca
    41701 tcaccagacg ctctttaaac ccggcgaccg gcgcaagggc aaacagctgc ggttttacgt
    41761 tttgctttaa ttcaaaaacc atccgtacgg tttgcgggtc aaattgcccg acgcgcgccg
    41821 acttgatgaa cgggtcgtct gcacgaattt gtgccgccat ccccttgagt accgagttca
    41881 ggtttacatc ttcgatatcc accaccacgc gttcagggtt actcaacgcg aactgcttat
    41941 atttcagctg acgatttgat tctaccgtca cgcgggtgta gctggacgca ggccagacgc
    42001 gcaccgccac gacctggcta accgcagcca ggctgacctg acttacgctc aatagccaca
    42061 tagcgcccgc gccttgcagt aaacgacgac ggctgattgc agtgttggat cctgacatgc
    42121 ctctcccgag caaaagaaat ctaagctgtg taacaagtaa acgactaatt tgaccggaaa
    42181 ctttagcgaa agacgcataa tctgtcatct aataaacggt aaacattctt tttatattca
    42241 cggcattact gataaaaaag tcgctctcgc ataaaattta cacttgcacc ctgcgaaaaa
    42301 acagaataaa aatacaataa tttcgaataa tcatgcaaag gggtgtgccg tggtaaagga
    42361 acgtaaaacc gagctggtcg agggattccg ccattcggtt ccctatatca atacccaccg
    42421 gggaaaaacg tttgtcatca tgctcggcgg tgaagccatt gagcatgaga atttctccag
    42481 tatcgttaat gatatcgggt tgttgcacag cctcggcatc cgtctggtgg tggtctatgg
    42541 cgcacgtccg cagatcgacg caaatctggc tgcgcatcac cacgaaccgc tgtatcacaa
    42601 gaatatacgt gtgaccgacg ccaaaacact ggaactggtg aagcaggctg cgggaacatt
    42661 gcaactggat attactgctc gcctgtcgat gagtctcaat aacacgccgc tgcagggcgc
    42721 gcatatcaac gtcgtcagtg gcaattttat tattgcccag ccgctgggcg tcgatgacgg
    42781 cgtggattac tgccatagcg ggcgtatccg gcggattgat gaagacgcga tccgtcgtca
    42841 actggacagc ggtgcaatag tgctaatggg gccggtcgct gtttcagtca ctggcgagag
    42901 ctttaatctg acctcggaag agattgccac tcaactggcc atcaaactga aagctgagaa
    42961 gatgattggt ttttgctctt cacagggcgt cactaatgac gacggtgata ttgtctcaga
    43021 acttttccct aacgaagcgc aagcgcgggt agaagcccag gaagagaaag gcgattacaa
    43081 ctccggtacg gtgcgctttt tgcgtggcgc agtgaaagcc tgccgcagcg gcgtgcgtcg
    43141 ctgtcattta atcagttatc aggaagatgg cgcgctgttg caagagttgt tctcacgcga
    43201 cggtatcggt acgcagattg tgatggaaag cgccgagcag attcgtcgcg caacaatcaa
    43261 cgatattggc ggtattctgg agttgattcg cccgctggag cagcaaggta ttctggtgcg
    43321 ccgttctcgc gagcagctgg agatggaaat cgacaaattc accattattc agctcgataa
    43381 cacgactatt gcctgcgccg cgctctatcc gttcccggaa gagaagattg gggaaatggc
    43441 ctgtgtggca gttcacccgg attaccgcag ttcatcacgg ggcgaggttc tgctggaacg
    43501 cattgccgct caggcgaagc agagcggctt aagcaaattg tttgtgctga ccacgcgcag
    43561 tattcactgg ttccaggaac gtggatttac cccggtggat attgatttac tgcccgagag
    43621 caaaaagcag ttgtacaact accagcgtaa atccaaagtg ttgatggcgg atttagggta
    43681 agcgatgaaa atcgccggat gcggcaggcg taacactcgt gcgtcgcatc cggcaattac
    43741 gtttatcccc gcgaactaaa caacgccgcc agaccactgc gccgctcagt acgagtggcg
    43801 attgccgcac ttaatatacg ctcatcggca tacagcgaca gacggcgacg cgcgcgggtc
    43861 accgcggtgt ataccagctc tcgcgttact atcggcgtgc gttggctcgg taaaatcaac
    43921 gccgcatggt cgaactccga tccctgcgat ttatgtaccg tcatcgccca cgtagtttcg
    43981 tgctctggca agcgactcgg ttgcacagac ttaatattgc cgtccggcat cgcaaaccag
    44041 acgcgcgtcc cctgcccgcg atccagcgca ataccgatat cgccattaaa caacccaagc
    44101 gcgctgtcat tacgggcaat catcaccggt cgaccttcgt accaacgaga gtgcgaatga
    44161 cgatgaattt tgcgcttctg ttgcataaac tgctcaattc gctcattcag tccagccacg
    44221 ccaaatggcc cttcccgcag ggcgcacaaa agttggtact cattgaacgc ctgaatgatt
    44281 aaatccggct cggcacgcgc ttgcagcaga tccagataac gtccataacc cgcaagagct
    44341 tcctcaagca tcgcaatata atcttcgccg ctctgtaaaa gccgtttttc aatatcagta
    44401 aaatcctgct gaaaaacggt tttcactgcc gttttatcac cacggttgat cgccgcagct
    44461 aactgaccaa tgccagaatc gctgccgaaa cgatagcttt tttgcagcag gcagagactg
    44521 tcgcgcaaag atgccgcttc tgtgccggtt cctgccggaa cgtgagttcc cgtcaggcgg
    44581 cttagctgcc cggcacgctc ggcggtaaag ccggcattgg cataagcgca gatatcgccc
    44641 agcacagccc cagcctcaac cgaggccagt tgatcacgat cgccgagaaa gatcactcgc
    44701 gcatgatcgg gcaaggcgtc gatcagtctc gacatcatag gcagatcgat cattgacgct
    44761 tcatctacca ccagcacatc aagatgcagc gggttaccgg catgatgacg taaacgctgg
    44821 ctacccggct gcgcgcccag caatcggtgc aaagtgctgg catcttccgg aatgcgtctc
    44881 ttttgttcat cggtcagcgg taactgtcgc aaagccttgc cgagagattc ggttaagcgc
    44941 gcggcagctt tacccgttgg tgcagccaga cggatacggc agcgttcgcc gtcggccatt
    45001 tgaattaacg ctgccagcaa cttcgccacg gtggtcgttt taccggtgcc tgggccgccg
    45061 gaaatcaccg agatccgccg cgtcagcgcc actgctgccg ccactttttg ccagttaatt
    45121 tcatcgctta ctggaaaaag tttgtccagg gtttgcgcca gtagagcttc atcaacctca
    45181 atggcatgat tcacctcatt gaagaagcgc gctaccgtcc gctcgttaca ccacatgcga
    45241 ttcaaataaa gacgatcgcc acagaggatc atcggcgtgg gttcatctcc cctgctgacc
    45301 gcttgagaag ccagcaagga ttcttcccaa ttttgtagct caccgatttc actgacacag
    45361 gtcgccaaca gcggatgcga cgcctcgtta ttttccagtc gtgaaagcgg caaacagacg
    45421 tgtccctctc cggcatcatg acttaacagt gccgccgcga gggtgacggc aggatgttca
    45481 tctcccgcca cggtcagggc aaattgcaca tccagcgggc gtagctgttt gtgctccaca
    45541 gcttccagta attgcttttg caatttcatt acgcctcctc cagggtcata ccggcaaaca
    45601 tctcatccat cagggcaatc aacccggcgt tgggtcgggt tgtgtaaatc ccttgttgcg
    45661 gatgttcttt atcaacgcca cgcaggaaca gatagataac gccgccaaag tgacgatcat
    45721 agtcgtaatc agcaatgcga tggcgcagat agcgatgcag cgccagggta taaagctgat
    45781 attgcagatc atagcggtgt gcctgcatcg ctgctgccat agcctgttgg gtgtaagccg
    45841 aactgtcttc acccagccag ttggatttat agtcgagcag gtaataacgc ccttcgtggc
    45901 ggaataccag gtcgataaag ccttttaaca tgccgcgtac ctgcatgaac tccagcggcg
    45961 ggcagccagc ggatagcggg tcaaactggc gaattagtgc atcaagctgg ctggcgatga
    46021 gcggttcgct aatcggcagg taaaactcca tctccacctg tttatcgcgg tcggaaagct
    46081 gactcaggct aacacccgtt tcattgagag gtgcctggag gacagccgtg atccactcgg
    46141 tcaacaccgg ttcccactgc gattcaaagc cgccgagttc cagtttttcc tgtacccagt
    46201 tcgggtcaac cggctgggta aaatcgaggt cttcaaacaa actgtgcaag aatgtccccg
    46261 gtgacgcacc gcgcgggaac tgatgcggtg ttaacgtcgg ttcttcaacg acgctgacca
    46321 cgcctgcggc atcaacatcc agccgtggca tcagatcctg agcgataccg tgaccacgct
    46381 gctgcaagcc ggagtagctg gtgacacgcc agttatcgcc gggcaatcgt tgtagtgtcc
    46441 tcgcattcag ttctgcagtt aaagcatcat taacctgcca gggctggtta tcaccagttt
    46501 gtgccgtttg ccaggcaata tcatcatcgc ataacgcttc aatacaggtg cgaagccctg
    46561 ccgcatcttg cggttcccct ttttgcagca aacgcccgag cgcactttgg tggacgtcgg
    46621 tgtcaccttt tttatcgcca cgacggcgca ccagcggtgc aacgccgaga ctgcaatgcc
    46681 aaaccgaacg cgtcagcgcc acgtaaagca aacgcagatc ttccgccaga cgttcgacct
    46741 ccgcgaggtc gacgctttct ggcgcagcat taagatccaa aactgcctca aacgagtggc
    46801 gatcgtgata aaacgcctga tcctggacgc ggaaattggt aataaatggc aaccagacca
    46861 gcggatattc cagccctttc gatttatgga tcgtgacaat ctgcaccaga tgtttatcac
    46921 tttcgaggcg catttgttgg ctggaggcat tactgtctgg ctcgaggata tgttgcgata
    46981 accagcgtac cagcgcatgt tcactttcca gctgcgttcc ggcttcttga agcagttcgc
    47041 tgatatgcaa gatatcggta agacgccgct caccgcctgc cgttgccagc aagttttctg
    47101 caatgttacg cgccgacatc agcgcccgca gcatcggcat aacgccacgt ttgcgccaga
    47161 tttgccgata accatcgaac tcttcgacta ccgcatccca cgcatgttcg tcattattca
    47221 gcgtttcaat atccagcgca ttcagcccca tcattgacgt tgccagcgca ctgcgcaggg
    47281 tgttctcacg ttcgggcgtc atcaccgcct gcaacagcca aagcatttcc tgcgcttcca
    47341 gagtttcaaa aacactatcg cggttcgaaa ggtaaacgga agggatttcc agcaacgtta
    47401 aggcatcgcg cacctgggcg gcctcctggc ggctgcgcac cagcacgctg atgtccgaag
    47461 cacgcaccgg acgcgcgtcg tcgccgttca tcagcaacgc ttcgccccgc tgtccggctt
    47521 gtagccagtc gcggatttgc gcagcacata cctgcgccat ggtactttga tagtcgccaa
    47581 cgccgcagct ttcgccttcc atcagccaca ttttcatcgc aggctgcgtt tcacctttaa
    47641 agacaaaacg taacgcctga tttttcccgg ctgatttcac tggaataaac ggtatttcgc
    47701 gaaacatgaa cgcgtcatca gtctggctga aaagcttgtt cacactgttc accattccag
    47761 gtgcggagcg ccagttggtg tctaaagtgt agtgggcgtg aacttcacta cgcgccttca
    47821 tataagtgaa gatatccgca ccccggaatg catatatggc ctgcttcggg tcgccaatca
    47881 gcaacaatgc ggtttctggc tgatggtgcc agatacggcg aaaaattcgg tactgctggg
    47941 ggtcggtatc ctgaaattca tcgatcattg ccaccgggaa tcgcgtacgg atcgccgctg
    48001 ccaacacctc gccgctttcg ctacgcagcg cggaatcgag ccgacttaac atgtcatcaa
    48061 aacccaattc gccacggcgg cgtttttcac gcgctactgt ttcgcggatc tcagccaatg
    48121 cgcgggtgat caccagatca cggatcgaca atggttctgc aagcagttga tcgatcgcct
    48181 caaacagtgg atgtcgcggg gtttcccccc cggctttcgt gcgatcttct aagaaacgct
    48241 gggagaattt ttccagcgac tccggcaact gataactgtt tgtctcttct tctgcccagg
    48301 cgctgatctt gtcgatccat ttagcctgat tgctacggtt aaacttgcgt cgatcaatac
    48361 cagaagattc gatcagcgca tccagttcac ccactgcgtc gcgccactgc tgttttaccg
    48421 tatcaatacg cgccacaatt tgcgcgtgac gggaagctag cgtttcatca tcgggcggcg
    48481 gtgctttgat aaccggcgct tcgccttgca gataacgatt aatatcgcgc agcaacgcct
    48541 gcggcccttt ccaggtttca aagacgacct gggctatttc acgcggcagc gggtagcagt
    48601 ggcgacgcca gaaatcggcg caggcctggt agcgtagcag agactcatct tcaatcagct
    48661 gctgctcaaa cagcatgccg gattcaaagg cattcaggtt gagcatgcgc tggcaaaagc
    48721 cgtgaatagt aaagactgcc gcttcatcca tctgccgttc ggctaacaac aaccactgcg
    48781 cggcttgcgc tttatcgtcg acctcttcca gcaggtgttc gtacaatgga ttgtcggtgg
    48841 tttcacgcag acaggcgatg cgcaactcgt ggatattgct acggatacga ccgcgcaatt
    48901 ctgccgtggc agcctcggta aaggtgacca ccagcagttc ttcaacggtc agcgggcggg
    48961 gaaaggcggc ggaaccgcct agtccaagta acaggcgcaa ataaagcgcc gcaatcgtaa
    49021 aggttttgcc tgtgccggca gaggcttcaa tcaggcgctc gccctgtaag ggcaagcgca
    49081 aaggatctag tgtctcggcg acatcactca ttcttttcac tcatcagggg cattgtttgc
    49141 tgcaacgcgc tgacgttctc ccacactttc cagccttcag gatgcacata ttcggctttc
    49201 ccgttttggc tgccggaaat ctgcgacagg atagccatgc cttgcggctc gactaccgcc
    49261 tgatggaaga aatcggccag tttttgcggc gtcagcagtt taatctgggc cacgatttta
    49321 tcacgcgaat cgaagcgcat attgccgcga tcgaaatctt tacttaactt cgatgcttct
    49381 tcgccgagcg tttgcggtgc ctgcagcatc tgggtaatta ccgcctgctg gatttgcgca
    49441 aactcatctg gcttcatcgc tcgcaatttt gcctctgcgg ttgggaaaaa cgccttgtaa
    49501 cgctcccaca agaatgaagg ctgtttatca ttgctttgca aaaggaagcc catgccccac
    49561 tgacgcccca cgctcattgg aaacgcaaac acggcatagc ccaattgttc ttcggtacgc
    49621 aactgattgt agaaccacgg ctgtacgatc tgccccaaca gagagctata ggctgagctg
    49681 gtgtattcat cgtagccagt cggtacaaat accgctgcca gtgcggagtc ggtgctgtta
    49741 ccggcttttt caaagatgac ggattgtttt ttatcgacca ctacatcttt gtttcgacac
    49801 cactctgaac catcagcgcc caactgtttt tgcacatcgc gtgccagcgt tgttgcctgg
    49861 gcctcggtca tgttgccgat aaccataaac tctggtcgag cccctgattt taaggcgtcg
    49921 cgataagcca gcacctcttt caacgtaatg gagggcaaaa ttttacgccg ttcatctcgc
    49981 gagaagtacg gcacttgcga gagcatctgc gcgggcataa tcgcctgctc aaacgcttta
    50041 cccttttctg cggaatccat catctggtta taccaggact tcgcctgctc aagctgatct
    50101 tccgtagcgg tatagctaaa gtacccctcg agcaatgcct ggaacagctg cggcagacgc
    50161 tgggtgtaac cattagcatt aaccataagg ccgttgttag cgttggtgga aaaacttatg
    50221 ccaccaaccg acgcctggtt gcttaactga tcaagcgcca gccctgcgag ataatcattg
    50281 agcgcaaaca tcacctgatt gcgggcgctg tccatggctt tcggattacg caaaatcagg
    50341 ctgacatcag ctttgggttc gctggaaaaa taacggcttg gcgcatacac cacgcgcaga
    50401 ttcgactcat caacaatcag ctctggatgg tcatatttct tctctgactt aatcagcgag
    50461 aaatcgtcag gaatataggg gttaagctcc ggtaaggaga gcgcaatatt ggcagctttt
    50521 tgctgccagt cagcgaaagt ttgttcgctg attttatcga cctgatacgg cgcatcgaca
    50581 aaataagccg ttttattgtg cggctctttc gggctgatat accagatacg cgcattctgc
    50641 ggcgtcatca tcgccagacg ttcttttact gctttagcat cgtaccgatc ggcaatattg
    50701 actgcatcca gcgtatgctc aaccggaacg cgaatcatgg tatctgccag ccattcgacg
    50761 taatccatat cacgggtgat cgacggataa cggaagtcga tatccagcac attcgccagt
    50821 tcatcgaagt attgtttatc aatgcctttt tcacgtaaca gattgagata gctaaaaatt
    50881 gccgccacaa cctgatcgcg attagccagg cctttatcgg ttaaagacgc agagatcgct
    50941 aatacgccgc tgttgccgtt gacgatagga tcggagttgg cgctaatgcc ctcaactaat
    51001 ccctgctttt gcagccagtc ggaaagtgta cctgggctgc gattaccaat cagataggta
    51061 atcaattcat ccgttttact acggaacttc gctgagttgt tatcgatgcg aaactcaacg
    51121 cgcagaactt tacgcggcat tgccgggacg taatgaatga taatgccctt ttgcgcgtcg
    51181 gtgactaccg gcacggtgat ttccggtttt ttgctctctt tgttcggcac gcgaccaaag
    51241 gtgtccgccg ccatttttgc caactccggc agcggtttat tgctgtaaat aaccgccttc
    51301 atcagattgg cggaatagta cttctcgtgg aaatctttca gcgcctgctg caccggatta
    51361 ccaggtttat cgcttaaagt ttcgaggtta ccaccagaaa actttgaacc ggggtgtgcc
    51421 gggttaatgg tttctgcgct gacctgtgcc atgcgcatcc cgtcacgcgt acgcgccatg
    51481 gttaattcag cgttcaccgc attacgctca cgttcggcat atttcttgtc gagcaaaggt
    51541 tcagcaatag catcggccag gcggtctacc gcaccaggca aggcgtcgtt ctcaacttcc
    51601 agatagaaag ccgtgcgata cggcgcagtg ctggcattgt gactaccgcc gtgcattttg
    51661 agatattcgg ccagactgtc agcctgcggg tactttttcg accccatcag actcatatgt
    51721 tcaaggtaat gtgccagccc ctggtacgcc tcgggatctt ccagcgaccc aacgggcacc
    51781 accagcgccg agagcgattt aactgcctgc ggatcagaaa ccagcaagac caccataccg
    51841 ttatccagac gtatagcctg atactggcgg ttatctttat cacttttacg gatggtttcc
    51901 tgaatcggct gccatcccgt ttctgcctga cttaagggtg cccaaagggc aactaacaac
    51961 aataatgctt tgaaccaggt gctgcggggc attctcggac ctcataatca acttaatttt
    52021 ctgtccagat tcaacacgtt aacgcaaatc atctgccaga atttaatctt gtgctgcacg
    52081 agtcagccta tgtttatata accatcagtc cgtgactggt gcgcatcata aagtaagcgg
    52141 atagattgcg caatttttat acagcactca tgactgatta aagcgaaaca gcggtaacag
    52201 gaaacgttgc gactgttcaa cgatggcctc cattgtctct ggtgttaatt gccgccagag
    52261 cctttgatac cagatatcat caccttcgcc acgcaccatc atgttacctt cgtaagcctg
    52321 aaggaatttc gtacgggctt tttgcaacgt ggaatcgtca tccagcatgg catcgttttg
    52381 cgcgtcataa caggttttta gccacgcgcc gccactttca ggtaacacca gcaatggcgc
    52441 ggacatccct tcacgatacc cctcaatcag ttgtgagagg taatgcaaag cctgttcggc
    52501 tgcaagcggc ggaaaacgcc actcgccgtc tttgcgtaga aaaaggcgac tttcaccatt
    52561 accaccgctg gcacagtaga caaggtgttc cagccaaagt tgcattccct gcgccacact
    52621 taataaagag ggacgccagc gcaacaggcc atccggctgc acctgcggca accagccagt
    52681 tatctgcaca ccgttgcagg cgagatcaat ttccatactc tgccccggct ggcgacaggc
    52741 aatgactctg tcggcaagct gctgcatctc ctggcactga gtttcccaga aaatttcacc
    52801 aaaagcgcca tacggtaaat cccctgccgc ccggaagcga cggaacaagc gttcggcatc
    52861 atcctgctca accagtgcat tcaataactg ctgattgatt tgataacggc taagcccttc
    52921 cagaataaat ggctcggtgt cggggatttc gctgtcttca gtacggaagt tcacctgcaa
    52981 acgcatctgg aagaacgccc gcaccggatg tgcccagaat cgttgtagcg tttccagcgg
    53041 cacggtttcc ggtaaggtaa acggcagcgg ctgaacaaat tcagaatgtg ctttaccagc
    53101 ctggctggcc gcaggtagcc attcacgagc atagctttgt cgttcgcctg gctgatagtt
    53161 ttgtggatca aacggcattc gggtatggag gcaagtaaga tgcgctttta cccttgcctc
    53221 gctttcatca cagttgagcg cttcatcgcc cggtagataa tgactttgcc cgatgtagtc
    53281 gatcagttcc tgcaccagta ccgacgggaa acgttcactg ttatcctgaa tggaacgccc
    53341 gatatagctg atatagagtt tttgctgcgc ggaaattaac gcttccagga acagatagcg
    53401 gtcgtcatcg cgacggctac ggtcgccacg cttcggtttc tggctcatca ggtcaaagcc
    53461 caatggcgca agctgacgtg gataaacgcc gtcgttcatt cccagcaggc aaaccacttt
    53521 gaacggaatt gaacgcattg gcatcagagt acaaatgtta accggtccgg cgagaaaacg
    53581 ctggctgata cgttcttgat ccagacgctg tgccagttca tcacgcaata gtgacagcgg
    53641 caccgcgtcg ccatactgcg cacctaaacc ttcggcgata atcgcctgcc attgttgttc
    53701 gatcagcgtc atcgccgctt cggtttccgc atccggcagg aagaaggcgc tgagcatatc
    53761 gcgacaaacc ggcaaccact cttctagcgg acgctcctgt gccagcccgc gacgccagat
    53821 gtttagctgc attagcagtg aagccagatg ccccaccagt tctgcaatta agccgctcga
    53881 ttcatcataa ggtagaaccg attgccactc gccctgcgcg ctctccatcg cgtagcccag
    53941 caacatacgc gtcaggccaa atcgccaggt gtgttgtccg gtggcgggga gttccagctc
    54001 gcgaacgttg tcgtcatcta tcccccaacg aatgccggat tcgttgaccc actggcgtaa
    54061 ataacgcagc ccttcttcgg tgatgtcaaa ccgcgccgcc agcaccggca catccagtaa
    54121 tgccagcacg tcttccgaca caaagcggct gtctggcaac gataacaggc tgataaacgc
    54181 ttccagtacc ggatgcgact gccgcgcccg acggtcggaa atggcgtaag gcaggtaacg
    54241 atccgcaggt gcgctaccaa acacagcccg aataaacgga ctgtagctgt cgatatcagc
    54301 caccatcacg atgatgtcgc gcggagtaag tgtcgggtct tcttccagca tcgccagcag
    54361 gcgatcgtgc aaaacttcaa cttcacgctg cgggctatgg caaacgtgga aggtgatact
    54421 gctatccagt ggatcaagcg ggcgtttgtt atcgctacgg gaaaactctt cgatgttcac
    54481 accagcaacg gcacggtttt ccagttccag aatgtcagac tgaatgttat gcagcaggtt
    54541 atctggcgtg acatcgacaa aggcgtccag ctcctggctg ctctccaggt cagaaaggag
    54601 ataaatgtag tcgcgcccaa gcttacccca tgaagccagc agcgggttgc cgacatcctg
    54661 ttcaccatcg ctgttaaaga gctgcccggc attttcgcta tcacgaaata gcggcaattc
    54721 gcgatcttca aaactgtgtc gacgctgacg ggtcagcagt ttcgccagat aagcaggatc
    54781 tttaatatcg ccccagtaat aacggcaggg gttggtaaac aggagatgga tttcaatatg
    54841 tttacccagc gcctgtagcg cctggagata aacaggcggt aacgcggaaa taccgcatat
    54901 aaagacgcgc gaaggtaacc ccggcgggca ggtcgtcgcg gactccagcg tttcgataaa
    54961 gcgctgatag agattggcgc ggtgccagcg cggttgcccg agttgatggg tatattccac
    55021 cagcgccttc cacaacgggg cttgccaggc ctgtgcttct cccagccctt caaccaaatg
    55081 tcctgtttcc cactgtgcca gccagtccgg acgatagacc agatactggt caaacaggtc
    55141 cgccgctttt gaggaaagct ggaacagttt tcgcttgtcg ctatcgtcag tcagataatg
    55201 ccgcaacagg gtaaagtctt cgcgctccag caattgcggc agcagagtca tcagtttcca
    55261 gctcatgctc tgtttgttaa aggcgctctc tttggggatt tccggtaaca cccggacgaa
    55321 catatcccag ataaagctcg ctggcagcgg aaaatcaatg tttgccgcaa tactaaactt
    55381 ttgcgacagg gtcatttgca gccactgcgc cataccggta ctttgcacca gaatcatctc
    55441 tggttcgaaa ggatcgtcca gccgttcgcg ttcgacaata aactccatca acgcttccag
    55501 cacgtccaga cgattggaat ggtagaccct taacatagcg gctcctgact actgacgatt
    55561 cgggcaatgc aggcgggtca tctcgccttc tctgcccccg ggtgaaacta gcgtaacgct
    55621 gatgctgaca catcccgcct gcgatgtctg cattcggttg acctgccagt tggcaggtgg
    55681 cgaaatcgcg cgcagttgcg tttgctgcca accatgccgc cagagctgtt ggtactggtt
    55741 tctgctggcg agactgttca ttaatgttcg ctggataccc gataacgcag tgacaatcat
    55801 caccatcagc accatcgcaa ccattacctc cggcaggcta aagccttgtt gattcttcag
    55861 ggaagctgac ataacgccct ctctttcagc ggacaaaaat cgctccagcc gcgtggcgaa
    55921 aaaacaatgt ttccatcaat gacttcgcct gttcgccaca acgaaacgcc ttcataaccg
    55981 gcaaccaata aggcttcatt atctgccagt aaacgcaaac aaacccgggc atcggttcca
    56041 gcgtactgcg agcactgaac tgcgggctgc gtctgccagg agtgcatttt cccccacgcc
    56101 agcgccgact gaacgatggc ctggcggcgc aatgactgac tttctatgct cacgcgagag
    56161 gcaaaactgc gatcctgctg actcattcct tgcaatagca agctacccaa aaccagcagc
    56221 atcaggacca gagccagtga cgaaacacct ttttcgcggt tcacaggttg aatcctgtca
    56281 cgctatagct ggcatccacc acggtttgcg gttcagactt actggcagca cgcatattaa
    56341 ccgtcaacac cggcgagaag ccgctgacat cctgacgtac gacctgaaaa gtgtcgataa
    56401 tgatggcatc cggattagtg actttatccc agcccttacc ttcacaggat gtcgcaccgc
    56461 gtagcgtttc cagcacatgc tccttcagac gaaatccaat ctggtcagac tcttttaccg
    56521 gttcgcgatc ccagataccg ttactgttcg catcccactg cacaatgata cagtcaccct
    56581 gtccgacaat ttccagccct tcgccggtac agctgccatg acaataaccc gccctctgga
    56641 gatgcttcgc gacggtaaat acccgcagcc agatttcatc ttccagcgcc agcttacggg
    56701 tgctcgttaa actttcacgc tgtaacgcag gcagaaagcg tgcagccccc agcaacaata
    56761 cgctactgat cgccatagca atcaacactt ccagcagaga aaaaccttgc tcttttacag
    56821 gcatccttct gtttctcctt gctgacaaag ccggagtctt ccccacggcg aaaccaccag
    56881 ccaccactcg cccgttgagt ttttgaagcg aatatgcccg gcccatgcgg tattgcgcag
    56941 gccaaagaaa gcaagcgaag gtgtcaggtc gctcatttcg acttcgggcc agcgtggcac
    57001 aaagaccaat ggtgaactgc catgacaggt attggcccca gcagcggaac tcacaaggca
    57061 ccataacgtc ccctccctga taacgctgat actgtggtcg cggttatgcc agttggcatc
    57121 ttcacgtaaa tagagcaaat agtcccgcgc ctggctggcg gtttgccata gccgttgcga
    57181 ctgctgccag tattgccagc catagagtcc acttgcgctt agcatgacca aaatcagcat
    57241 cgcgaccagc gtttcaatca gcgtataacc acgttgtgtt ttcatgccgg cagtatggag
    57301 cgaggagaaa aaaagacgag ggccagtttc tatttcttcg gcgcatcttc cggactattt
    57361 acgccgttgc aggacgttgc aaaatttcgg gaaggcgtct cgaagaattt aacggagggt
    57421 aaaaaaaacc gacgcacact ggcgtcggct ctggcaggat gtttcgtaat tagatagcca
    57481 ccggcgcttt aatgcccgga tgtggatcgt agccttcaat ctcaaagtct tcgaaacggt
    57541 agtcgaagat ggattcgggt ttacgtttga taatcaactt cggcagcgga cgcggttcgc
    57601 ggcttaattg cagatgagtt tgatccatat gattgctgta caggtgcgta tcgccaccgg
    57661 tccagacaaa atcacccact tccagatcgc actgctgcgc catcatatgc accagtaacg
    57721 cgtagctggc aatgttgaac ggcaggccga ggaagacgtc acaggagcgc tgataaagct
    57781 ggcaagagag tttgccgtct gccacataga actggaagaa tgcatggcac ggtgccagcg
    57841 ccattttatc cagttcgcct acgttccacg ctgaaacaat aatgcggcgg gaatccgggt
    57901 cgtttttcag ctggttcagt accgtagtga tctggtcaat atgacgacca tctggcgttg
    57961 gccaggcgcg ccactgttta ccatacactg gtccgaggtc gccgttttca tcggcccatt
    58021 cgtcccagat ggtgacattg ttttcgtgta gataagcaat gttagtgtcg ccctgcagaa
    58081 accacagcag ttcgtggatg atggaacgca ggtggcaacg tttagttgtc accagcggga
    58141 atccatcctg caggttaaaa cgcatctgat gaccaaaaat ggaaagcgtt ccggttccgg
    58201 tacggtcgtt tttctgtgtg ccttcgtcga gcactttttg catcagttct aaatactgtt
    58261 tcatggttcc tcaggaaacg tgttgctgtg ggctgcgacg atatgcccag accatcatga
    58321 tcacacccgc gacaatcatc gggatggaaa gaatttgccc catgctgatg tactgcaccc
    58381 aggcaccggt aaactgcgcg tcgggctggc ggaaaaactc aacaatgatg cgaaacgcgc
    58441 cgtagccaat caggaacaag ccagagacag ctcccattgg gcgcggttta cgaatataca
    58501 ggttgaggat aataaacagc accacacctt ccagcagcag ctcgtaaagc tgtgatgggt
    58561 ggcgcggcag cacaccgtaa gtgtcgaaaa tggattgcca ctgcgggttg gtttgcagca
    58621 gcaaaatatc ttctgtacgg gagcctggga acagcatggc aaacgggaag ttcgggtcaa
    58681 cgcggcccca caattcaccg ttaataaagt tgcccagacg cccggcacca agaccaaacg
    58741 gaatgagtgg tgcgataaaa tcagaaacct ggaagaagga acgtttagta cggcgggcaa
    58801 agataatcat caccacgata acgccaatca ggccgccgtg gaaagacatg ccgccgtccc
    58861 agacacggaa cagatacagc ggatcggcca taaactgcgg gaaattgtag aacagaacat
    58921 aaccaatacg tcccccgagg aagacgccga ggaagcccgc atagagtaag ttttcaactt
    58981 catttttggt ccagccgctg cccggacgat tcgcccgtcg tgttgccagc cacattgcaa
    59041 aaatgaaacc caccagatac atcaggccgt accagtgaag cgccacgggt cctattgaga
    59101 aaatgaccgg atcaaactcc ggaaaatgca gatagctact ggtcatctgt caccacaagt
    59161 tcttgttatt tcgctgaaag agaacagcga ttgaaatgcg cgccgcaggt ttcaggcgct
    59221 ccaaaggtgc gaataatagc acaaggggac ctggctggtt gccggatacc gttaaaagat
    59281 atgtatatga tccgcgctat aaccctccgc gaatcagccc gcccatgccg cgacgctcca
    59341 taaaggctgc aacctgatgg cgaacttcgg tcgccagttg cgcttccaga ctacgctgcg
    59401 caagattttc tgcttcggca taatcaatgc gccgcagcag gtattttacc cgcgctacag
    59461 aacgtccgtt catagacaga tggcgatacc caagcccaat gaggattgcc acgcacatgg
    59521 gatcgcccgc catttcaccg cacaaacgga gatcgattcc atgtatttcc gcttcccggg
    59581 cgatcatcgc cagagctcgt aacattgcag gatgaagact gtcataaatg ttcgccaccc
    59641 gggtattgtt gcgatcaacg gccagaatgt attgagtcag atcgttggtg ccaacagaga
    59701 tgaaatcgac ccgctttgcc agatgcggca gcataaatac cattgacggc acttccagca
    59761 tgatgccgat acgtggtttg ggaatttcgt aaccgatcat ctcctcgact tcacgtccgg
    59821 cacgttcaat caggcggcgt gcttcatcaa cttcatcgag gcttgtgacc atcggcaaca
    59881 gaatattcag gttgcccgta gcggcattag cacgcagcat cgcccgcacc tggatcaaga
    59941 agatctccgg ctgatcgagc gtaatgcgaa tcccacgcca acccaggcat ggattctctt
    60001 cgctgatcgg catgtaaggc agctgcttat ctgctccgac atccagcgta cgcaaggtga
    60061 cgggtttatc attgaacatt tgcagcatcc cctgatactg cgccacctgt tcttcttccg
    60121 acgggaaacc actttgcagc atgaatggga tttcagtgcg ataaagaccg atgccatcaa
    60181 tacggctgcc cagtttttct tcatgttccg ggcttaaacc agcattgagc atgactttta
    60241 tacgctcacc gctttttaac tgcgcgggta aattgacgtc atcttccgcc agacggctaa
    60301 gctcaatctc ttcactaatt agccgctgat attcttgcag cagtaccggc tccggatcga
    60361 ccagcaattc accgcgatag ccatcaacga tcagcgtcct acgatgcagc accgaaggct
    60421 gaatatccgc gcccatcacg gtagggatcc ccagcgcacg taccatgatc gcagcatggg
    60481 agttggctgc gccatctcgc acgacaacac cgactaagcg atcctggggc agctcagcaa
    60541 gagttgtcgc tgacagttca tctgccacca gaatgaaacg ttccggccag gcgttcggcc
    60601 cttgattagc gtcatcaaga tgaaacagca atcgctgacc cagcgcacgt aaatcgccag
    60661 cccgctcttt gagatagtta tcgcttagcg cggcaaactg ttcggcaaat ttttcaatga
    60721 ccgtttttac cgcccactct gccaccgagc ctttatcaac ctcggcaaac aattcgcgac
    60781 gcagccgggt gtccgaaagt aggtgcgagt aaagatcgaa aatagccgcc gtttcttttt
    60841 gtgctccggc ggcaaagcgt ttgctgtagc ggcgaaactc gtttgccgct tcttccagcg
    60901 ccccggtcag tcgttcgcgt tccagagccg gatccagcgt tgatgcctga tacacctgtt
    60961 ccattaaagg taacgtggca tcctgccagc cttcggcaat cgccacacca ggtgctgccg
    61021 gtaatgcgcg gattcgcgtc tggcgatatt gcccaaacaa ggcagtcaac tgcgactgag
    61081 aaagaatagc tgccatctgg gtggcaagcg tcaccaggaa ggattcttca ctttcgtcat
    61141 actggcgcaa ctctcgttgc tgtaccacca gtacaccaag caactggcga cgttgaataa
    61201 ttggtacgcc taaaaacgcg cggaaacgtt cttcttttac ggaggggatg tatttgaagc
    61261 tggggtgctt ttgcgcatct gcaaggttta tcggttccgc cagcctgcca accaggccga
    61321 cgatcccttc atcaaacgcg agcgttacag tgcgaccgcg tggttttttc agcccccggg
    61381 tcgccatcag gtagtaacaa cgtcgatcat gatcggccag gtagaccgaa cagacctcgg
    61441 tatccatcgc aagacagatg tcggtaacca gaatatttaa cgcctcattc aggcgtggtg
    61501 cgctggctac cttttcgact atttcgcgca ggcgagtgag cataattggc gtgacttaac
    61561 ctcttttacg tcgataagca gatgtgtttt gtggttttgg cgtattttcc tgcagtgaca
    61621 tcaccacact cgcgaactct ttcattaccc tacggtagac atcacgttta aatgacacca
    61681 cctgtctgac cggataccag taacttaccc atcgccagcc gtcaaactct ggtgtactgc
    61741 tggtttgcat attgatttct gcatcgccgc tcaccagctg caagagaaac catttttgtt
    61801 tttggccgat acaaaccggc ttcgtgtccc aacgcaccaa acgtttcggt aatttgtagc
    61861 gcaaccagtt acgcgttgaa gcaaggattc gaacgtcttt gcggcttaat cctacttctt
    61921 caaacaattc acggtacatc gcctgctctg cggattctcc ggggttgatt ccgccttgcg
    61981 gaaattgcca ggagtgctga ccaaatcgcc gggcccacat tacctgcccc tggcgattac
    62041 aaatcacgat acctacgttt gggcggtagc catcgtcatc aatcaccgga ctacctcaaa
    62101 ataaagcttt atatacgaat gattgtttca tactccaaga agacggtaaa ccactctctg
    62161 cagggcatta cacactaata acaattgaat aactcacagt tatgtgcaga gttataaaca
    62221 gaggaagggg tggatagccg tttttattca ccttttctgt ggatagagtt gtgaagaact
    62281 acggaattac tacgggaaaa cccggagcat tctgaataag cccgatatag acattttaaa
    62341 atattcatta ttagtcaatg cattacatgt aatttgagta atgaaaaaga gtaattcgtg
    62401 acccaggtca cacctctcat ttacgggttg gcgaaagatc aaccaatgcc gtatttatcc
    62461 acagaatgtg ccactaagtt aagcactgaa ccactaaaaa ctggagtttc gtcgcacgtc
    62521 aaggctgtaa atggaaacag tagtggaggt ttttcacagt tatcccagct ttctgtggat
    62581 aacatggtgt aagatcctgt ttattttcag tgaccagatt tggaaaacct attgcagtgt
    62641 tgcgcaactc gattaccggc agcctaaaaa gcaatataaa tcagagaatt gaacaacaca
    62701 tgtggaaaaa gttacactgc gaatattcgg cacataattg ctgtttgttt tttaaccaag
    62761 gtatcatgac atgtcccaac ctcgcccact cctctctcct cccgaaactg aagaacagtt
    62821 gttagcgcaa gcacagcaac tttctggtta tacattggga gaactggcgg cacttgccgg
    62881 gctggttacg ccagagaatt taaaacgcga taagggctgg attggcgtgt tactggagat
    62941 ctggctaggt gccagcgcag ggagtaaacc tgagcaagat tttgctgctc tgggcgtgga
    63001 acttaaaact atccctgtgg atagtcttgg tcgtccgctg gaaacaacat tcgtttgtgt
    63061 tgccccgtta acgggtaata gcggggtgac ctgggaaacc agccatgtgc gccacaagct
    63121 caaacgcgta ctgtggatac cggttgaagg tgagcgcagc atcccgctgg cgcagcgtcg
    63181 cgtaggatca ccgttgctgt ggagcccgaa tgaagaggaa gaccgacagc tacgcgaaga
    63241 ctgggaagaa ttaatggata tgattgttct cggtcaggtt gagcggatca ccgctcgtca
    63301 cggggagtat ttacagatac gaccgaaagc agcgaatgcg aaagcgctta ccgaagccat
    63361 tggtgcccgg ggcgaacgga ttctgacgct gccacgcggc ttttatttga agaagaattt
    63421 caccagtgca ctactggccc gtcattttct gatccagtag ccatcgcttt gacctgccgc
    63481 tttccgggca tataattacc gcttcatttt tttggcaggg ctttttagat gttatttgca
    63541 tggataaccg atcctaacgc ctggcttgcg ctcggtacgc tgacgctgct ggagatcgtt
    63601 cttgggatcg acaatattat tttcctttct ctggtggtgg caaagctccc cacagcacaa
    63661 cgtgctcatg cgcgccgtct ggggttggcg ggagccatgg ttatgcgtct ggcgctgctg
    63721 gcatcaatcg cctgggttac gcgcctgaca aatccgcttt ttacaatatt cagtcaggaa
    63781 atttccgccc gtgatttgat tctgcttctg ggtggcttgt tccttatctg gaaagccagc
    63841 aaggaaatcc acgaatccat tgaaggtgaa gaagaagggc tgaaaacacg cgtttcatca
    63901 ttcctcggcg ctatcgtgca gattatgctg ctggatatta tcttcagcct cgactcggtg
    63961 attaccgctg tgggtctgtc agatcacctg tttattatga tggccgccgt ggtgattgcc
    64021 gtaggcgtga tgatgttcgc cgcgcgctcg attggtgatt ttgtcgaacg ccatccttcg
    64081 gtaaaaatgc tggcgctctc tttcctgatt ctggtcggct ttaccctgat tctggaaagt
    64141 ttcgacatcc acgtaccgaa aggttacatc tacttcgcga tgttcttctc tattgcggtt
    64201 gaaagcctca ac
//
LOCUS       DAJVRF010000005        60486 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJVRF010000005 DAJVRF010000000
VERSION     DAJVRF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60486
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.005"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            108..902
                     /gene="panB"
                     /locus_tag="OQN13_000280"
     CDS             108..902
                     /gene="panB"
                     /locus_tag="OQN13_000280"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCS1613749.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            914..1765
                     /gene="panC"
                     /locus_tag="OQN13_000281"
     CDS             914..1765
                     /gene="panC"
                     /locus_tag="OQN13_000281"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCS1613750.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2057..2953)
                     /gene="rpnC"
                     /locus_tag="OQN13_000282"
     CDS             complement(2057..2953)
                     /gene="rpnC"
                     /locus_tag="OQN13_000282"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCS1613751.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3227..3607
                     /gene="panD"
                     /locus_tag="OQN13_000283"
     CDS             3227..3607
                     /gene="panD"
                     /locus_tag="OQN13_000283"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCS1613752.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3611..4840)
                     /locus_tag="OQN13_000284"
     CDS             complement(3611..4840)
                     /locus_tag="OQN13_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCS1613753.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(4904..5344)
                     /locus_tag="OQN13_000285"
     CDS             complement(4904..5344)
                     /locus_tag="OQN13_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCS1613754.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5449..6219)
                     /locus_tag="OQN13_000286"
     CDS             complement(5449..6219)
                     /locus_tag="OQN13_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1613755.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6216..7142)
                     /locus_tag="OQN13_000287"
     CDS             complement(6216..7142)
                     /locus_tag="OQN13_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1613756.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7251..7913
                     /gene="can"
                     /locus_tag="OQN13_000288"
     CDS             7251..7913
                     /gene="can"
                     /locus_tag="OQN13_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCS1613757.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(7954..8490)
                     /gene="hpt"
                     /locus_tag="OQN13_000289"
     CDS             complement(7954..8490)
                     /gene="hpt"
                     /locus_tag="OQN13_000289"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCS1613758.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            8696..11086
                     /gene="gcd"
                     /locus_tag="OQN13_000290"
     CDS             8696..11086
                     /gene="gcd"
                     /locus_tag="OQN13_000290"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCS1613759.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11133..12683)
                     /gene="cueO"
                     /locus_tag="OQN13_000291"
     CDS             complement(11133..12683)
                     /gene="cueO"
                     /locus_tag="OQN13_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCS1613760.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12849..13196
                     /locus_tag="OQN13_000292"
     CDS             12849..13196
                     /locus_tag="OQN13_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCS1613761.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13302..14168
                     /gene="speE"
                     /locus_tag="OQN13_000293"
     CDS             13302..14168
                     /gene="speE"
                     /locus_tag="OQN13_000293"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCS1613762.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14184..14978
                     /gene="speD"
                     /locus_tag="OQN13_000294"
     CDS             14184..14978
                     /gene="speD"
                     /locus_tag="OQN13_000294"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCS1613763.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15016..15378)
                     /locus_tag="OQN13_000295"
     CDS             complement(15016..15378)
                     /locus_tag="OQN13_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCS1613764.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15553..18150)
                     /gene="acnB"
                     /locus_tag="OQN13_000296"
     CDS             complement(15553..18150)
                     /gene="acnB"
                     /locus_tag="OQN13_000296"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCS1613765.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKIEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18505..20262
                     /locus_tag="OQN13_000297"
     CDS             18505..20262
                     /locus_tag="OQN13_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCS1613766.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20333..21757)
                     /gene="lpdA"
                     /locus_tag="OQN13_000298"
     CDS             complement(20333..21757)
                     /gene="lpdA"
                     /locus_tag="OQN13_000298"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCS1613767.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22082..23974)
                     /gene="aceF"
                     /locus_tag="OQN13_000299"
     CDS             complement(22082..23974)
                     /gene="aceF"
                     /locus_tag="OQN13_000299"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCS1613768.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(23989..26652)
                     /gene="aceE"
                     /locus_tag="OQN13_000300"
     CDS             complement(23989..26652)
                     /gene="aceE"
                     /locus_tag="OQN13_000300"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCS1613769.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26813..27577)
                     /gene="pdhR"
                     /locus_tag="OQN13_000301"
     CDS             complement(26813..27577)
                     /gene="pdhR"
                     /locus_tag="OQN13_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCS1613770.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28118..29491
                     /gene="aroP"
                     /locus_tag="OQN13_000302"
     CDS             28118..29491
                     /gene="aroP"
                     /locus_tag="OQN13_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCS1613771.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29534..30388)
                     /gene="ampE"
                     /locus_tag="OQN13_000303"
     CDS             complement(29534..30388)
                     /gene="ampE"
                     /locus_tag="OQN13_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCS1613772.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30385..30936)
                     /gene="ampD"
                     /locus_tag="OQN13_000304"
     CDS             complement(30385..30936)
                     /gene="ampD"
                     /locus_tag="OQN13_000304"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCS1613773.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31024..31917
                     /gene="nadC"
                     /locus_tag="OQN13_000305"
     CDS             31024..31917
                     /gene="nadC"
                     /locus_tag="OQN13_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCS1613774.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32120..32560
                     /gene="ppdD"
                     /locus_tag="OQN13_000306"
     CDS             32120..32560
                     /gene="ppdD"
                     /locus_tag="OQN13_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCS1613775.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32570..33956
                     /gene="gspE"
                     /locus_tag="OQN13_000307"
                     /pseudo
     CDS             32570..33956
                     /gene="gspE"
                     /locus_tag="OQN13_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            33946..35148
                     /gene="hofC"
                     /locus_tag="OQN13_000308"
                     /pseudo
     CDS             33946..35148
                     /gene="hofC"
                     /locus_tag="OQN13_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(35183..36226)
                     /gene="guaC"
                     /locus_tag="OQN13_000309"
     CDS             complement(35183..36226)
                     /gene="guaC"
                     /locus_tag="OQN13_000309"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCS1613776.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(36382..36426)
                     /locus_tag="OQN13_000310"
     CDS             complement(36382..36426)
                     /locus_tag="OQN13_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1613777.1"
                     /translation="MIIKTLKMSARKRN"
     gene            36451..37071
                     /gene="coaE"
                     /locus_tag="OQN13_000311"
     CDS             36451..37071
                     /gene="coaE"
                     /locus_tag="OQN13_000311"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCS1613778.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37071..37814
                     /gene="zapD"
                     /locus_tag="OQN13_000312"
     CDS             37071..37814
                     /gene="zapD"
                     /locus_tag="OQN13_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCS1613779.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37824..38021
                     /gene="yacG"
                     /locus_tag="OQN13_000313"
     CDS             37824..38021
                     /gene="yacG"
                     /locus_tag="OQN13_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCS1613780.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38121..38510)
                     /gene="mutT"
                     /locus_tag="OQN13_000314"
     CDS             complement(38121..38510)
                     /gene="mutT"
                     /locus_tag="OQN13_000314"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCS1613781.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38570..41275)
                     /gene="secA"
                     /locus_tag="OQN13_000315"
     CDS             complement(38570..41275)
                     /gene="secA"
                     /locus_tag="OQN13_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCS1613782.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41337..41849)
                     /gene="secM"
                     /locus_tag="OQN13_000316"
     CDS             complement(41337..41849)
                     /gene="secM"
                     /locus_tag="OQN13_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCS1613783.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42080..42997)
                     /gene="lpxC"
                     /locus_tag="OQN13_000317"
     CDS             complement(42080..42997)
                     /gene="lpxC"
                     /locus_tag="OQN13_000317"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCS1613784.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43098..44249)
                     /gene="ftsZ"
                     /locus_tag="OQN13_000318"
     CDS             complement(43098..44249)
                     /gene="ftsZ"
                     /locus_tag="OQN13_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCS1613785.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44310..45572)
                     /gene="ftsA"
                     /locus_tag="OQN13_000319"
     CDS             complement(44310..45572)
                     /gene="ftsA"
                     /locus_tag="OQN13_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCS1613786.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45569..46399)
                     /gene="ftsQ"
                     /locus_tag="OQN13_000320"
     CDS             complement(45569..46399)
                     /gene="ftsQ"
                     /locus_tag="OQN13_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCS1613787.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46401..47321)
                     /gene="ddlB"
                     /locus_tag="OQN13_000321"
     CDS             complement(46401..47321)
                     /gene="ddlB"
                     /locus_tag="OQN13_000321"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS1613788.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47314..48789)
                     /gene="murC"
                     /locus_tag="OQN13_000322"
     CDS             complement(47314..48789)
                     /gene="murC"
                     /locus_tag="OQN13_000322"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCS1613789.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48843..49910)
                     /gene="murG"
                     /locus_tag="OQN13_000323"
     CDS             complement(48843..49910)
                     /gene="murG"
                     /locus_tag="OQN13_000323"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCS1613790.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49907..51151)
                     /gene="ftsW"
                     /locus_tag="OQN13_000324"
     CDS             complement(49907..51151)
                     /gene="ftsW"
                     /locus_tag="OQN13_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCS1613791.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51151..52467)
                     /gene="murD"
                     /locus_tag="OQN13_000325"
     CDS             complement(51151..52467)
                     /gene="murD"
                     /locus_tag="OQN13_000325"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCS1613792.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52470..53552)
                     /gene="mraY"
                     /locus_tag="OQN13_000326"
     CDS             complement(52470..53552)
                     /gene="mraY"
                     /locus_tag="OQN13_000326"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCS1613793.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53546..54904)
                     /gene="murF"
                     /locus_tag="OQN13_000327"
     CDS             complement(53546..54904)
                     /gene="murF"
                     /locus_tag="OQN13_000327"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCS1613794.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54901..56394)
                     /gene="murE"
                     /locus_tag="OQN13_000328"
     CDS             complement(54901..56394)
                     /gene="murE"
                     /locus_tag="OQN13_000328"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCS1613795.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(56381..58147)
                     /gene="ftsI"
                     /locus_tag="OQN13_000329"
     CDS             complement(56381..58147)
                     /gene="ftsI"
                     /locus_tag="OQN13_000329"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCS1613796.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58163..58528)
                     /gene="ftsL"
                     /locus_tag="OQN13_000330"
     CDS             complement(58163..58528)
                     /gene="ftsL"
                     /locus_tag="OQN13_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCS1613797.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58525..59466)
                     /gene="rsmH"
                     /locus_tag="OQN13_000331"
     CDS             complement(58525..59466)
                     /gene="rsmH"
                     /locus_tag="OQN13_000331"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCS1613798.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59468..59926)
                     /gene="mraZ"
                     /locus_tag="OQN13_000332"
     CDS             complement(59468..59926)
                     /gene="mraZ"
                     /locus_tag="OQN13_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCS1613799.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14435 a        16759 c        14901 g        14391 t
ORIGIN      
        1 ccaccgcacc gccatcatct ggcggtgcgg taattgataa atcattgccc ccaccaatgt
       61 gactgataaa atgccaggca acacgacatc atttatcagg acacgttatg aaaccgacca
      121 ccattgcttc acttcagaaa tgtaagcagg ataaaaagcg tttcgcgacc atcaccgctt
      181 acgactacag cttcgcgaaa ctatttgctg atgaaggact taacgtcatg ctggtaggcg
      241 actcgctggg catgacggtt cagggtcacg actccaccct gccggtgacc gttgccgata
      301 tcgcctacca cactgcctcc gtacgtcgtg gtgcgccaaa ctgcctgctg ttggctgacc
      361 tgccgtttat ggcgtatgcc acgccggaac aagcctttga aaacgccgca acggttatgc
      421 gtgccggtgc caatatggtc aaaattgagg gcggtgagtg gctggtcgaa accgtaaaaa
      481 tgctgaccga acgtgccgtt cctgtatgtg gtcacttagg tttaacccca cagtcagtga
      541 atattttcgg tggctacaaa gttcaggggc gcggcgatga agcgggcgat caactgctca
      601 gcgatgcatt agccttagaa gccgctgggg cacagctgct ggtgctggaa tgcgtgccga
      661 ttgaactggc aaaacgtatt accgaagcac tggcgatccc ggttattggc attggcgcag
      721 gcaacgtcac tgacgggcag atcctcgtga tgcacgacgc cttcggcatt accggcggtc
      781 acattcctaa attcgctaaa aatttcctcg ccgaaacggg cgacatccgc gcggctgtgc
      841 ggcagtatat ggctgaagtg gagtccggcg tttatccggg cgaagaacac agtttccatt
      901 aaggagtcac gttgtgttaa ttatcgaaac cctgccgctg ctgcgtcagc aaattcgccg
      961 cctgcgtatg gaaggcaagc gcgtggcgct ggtgcctact atgggtaatc tgcacgatgg
     1021 ccatatgaag ctggtcgacg aagccaaagc ccgcgccgat gtggtcgtcg tcagtatttt
     1081 cgttaacccg atgcagttcg accgcccgga agatctggca cgttatccac ggaccttgca
     1141 ggaggactgc gagaagctaa acaaacgtaa agtggattta gttttcgccc cttcggtaaa
     1201 agagatctac ccgaacggta ctgaaaccca tacttacgtt gacgttcccg ggctttcgac
     1261 catgctggaa ggcgcgagcc gtccggggca ttttcgcggc gtttcgacta tcgtcagcaa
     1321 gctgtttaac ctggtccagc cggacatcgc ctgcttcggt gaaaaggact ttcagcaact
     1381 ggcgctaatc cgcaaaatgg ttgccgatat gggcttcgat attgaaattg tcggcgtccc
     1441 gattatgcgc gccaaagatg gccttgcgct cagttcccgt aatggttatc tgacggcgga
     1501 acaacgcaaa attgcgcccg gtctgtacaa agttttaagt tcaattgccg acaaattgca
     1561 ggctggcgag cgggatcttg atgaaattat tgccattgca gggcaagaac tgaatgaaaa
     1621 aggcttccgc gccgatgata ttcagattcg cgatgccgac acgttgctgg aagtctctga
     1681 aaccagcaaa cgagctgtaa ttctggtagc cgcctggctt ggcgatgccc gcctgatcga
     1741 caacaaaatc gtcgaactgg cgtaatgctt ttactctaaa taattcaagt tgcaggaagg
     1801 cggcaagcga gtgacaaatt cgtcaggaac gaatttgccc agccgcaggc tggccttcgg
     1861 tgagagacat ggatgtctct cattaatccc caggagctta cataagtaag tgactggggt
     1921 tagcgaacgc agacgcagca catgcaattt aaagtatgac gagtataact ggcgctacgg
     1981 ctgatggcgc caattattaa tttgccccac gtcatggcga tggcgtctgt aagcaattat
     2041 tcataacgca gacaacttag ttaatcagcc tatcaatctc agcaagagat aaatttgtca
     2101 tctctgcaac gtcttcccga gacatccctt tactcagaaa acgctgggcg aattcttgcc
     2161 tttcctcctg tcttccctgc tgaatcccct tctcaatccc tttctcttca aaccactgcg
     2221 ccagcgtcat catagactcc cctcccgttt ccctgtctct cagcacacca taaaacaaat
     2281 ccgcttgttc agtatgaccg cgttgcagca tatagttttg catggcaact aactgacttc
     2341 cgctagtgta cccttcgtca atcagcgtga ccagttgctc cagcaataac attaagtcgc
     2401 gctggcgaat atgtttttgc agtagttcga gaatcgcaat ccgccgatgt tgcatgattt
     2461 cgtcatccgg tgtgatggtg atatccacca gcgggaaagg actgttatag acgcgtcgtg
     2521 ccagctccgg cgagtaaaat atatcaaacc agcacattga gagcggataa ggtgtggcct
     2581 cgccctgata aaacagtatc ggcaccacca gcggcagctt atcgtgatcc gcctccagat
     2641 gccggtgcat ggcagcaata gaataacgca tcatgcgaaa gaccattttc ttatccggct
     2701 tgctttggtg ttcaatcaca acatgcagat aaccgggatt gccctgcatt tgcacggaat
     2761 agagcacgtc cgtgctgtgt cctttcaggc tctcttcaat gaaactcccc gactctaaat
     2821 gaagcgtgtt gaggtcacaa agttctcgta attccactgg caaatgtatc tccagaaagt
     2881 cgcgagccgt ctccgcgtgc attaaaaatt gtttaaacac tgcatcatgc ggtgtggtac
     2941 tcggtgcatc catcttccct gccttccgtg ttgtcttgcg tgaaggcagt atccagtcaa
     3001 ggcgtgccat cgtcagccac ctctttagtg tttaccgagc agcgttcaga gaaatttcaa
     3061 ttggttacag ccgttacatg gcacgcttcg gcacggtacg caaactgctg atgtaaatgc
     3121 cgtcagatga gtagacacta aacaaaaatc gggcaatact gcgtgagaat ttcctcaaag
     3181 caggcatcgc ctgcttcgtt aacgacaggg tagaaaggta gaagttatga ttcgcacgat
     3241 gctgcagggc aaactccacc gcgtgaaagt gactcatgcg gacctgcact atgaaggttc
     3301 ttgcgccatt gaccaggatt ttcttgacgc agccggtatt ctcgaaaacg aagccattga
     3361 tatctggaat gtcaccaacg gcaagcgttt ctccacttat gccatcgcgg cagaacgcgg
     3421 ttcgagaatt atttctgtta acggtgcggc ggcccactgc gccagtgtcg gcgatattgt
     3481 catcatcgcc agcttcgtta ccatgccaga tgaagaagct cgcacctggc gacccaacgt
     3541 cgcctatttt gaaggcgaca atgaaatgaa acgtaccgcg aaagcgattc cggtacaggt
     3601 tgcttgattg ttatccctgc ggctggttac tcaccagccg cgacatcgtc tccagcgaat
     3661 ccgttcttaa gatataaagt cgttttagta acaacggatt atcccccggt tttactttgc
     3721 ctttcatggt tgtcaccgcc aggtgaaatc cggcatcgtt tgctgccttc acggctttgt
     3781 cattaaatcc gccaaacgga tacgaaagat accagacatg cggattaaat tgcgccagag
     3841 cgcggcgtga acgtgcaaaa tcaaacagaa tattgtgctc actacggctc agtaatatgg
     3901 gtcggcgata gccatccacc cgatgcaaaa aatgggtatg tgactgaaaa tcaaaaacat
     3961 cgcgaatttc gttaagctca gaaacgctca taaattgcag cgattttggg ttccacttct
     4021 gcgggtgacg tttgatgcgc gaggtaacaa caaacgccgt cgccttcatg ccatattgtt
     4081 tcaatacagg atacgcatag cggctcaccg acttgaggcc atcatcaaag gtaatcacca
     4141 ccgctcgcgc agggagattg atcttattct tcacgtaacc ttccagctgc gccatgctca
     4201 gtgtcgcgta tcccctgtcg cgcagccagg ccatttggtt attgaaagcg cgtaccgagg
     4261 tggtcgtcga agtatggcga aaacgggtgt tttcttcgtc gcgcagaata tggtgataag
     4321 ttagcaccgg aaggccatta tcgggttggg catccagcgc gctgatatag gccagtcgat
     4381 cgccaatacg gacctgatac caggtttgat ttaacctgtc tttcagttta tgcaaaatcg
     4441 ggtagcgcaa attgtccgcc aataccccaa atggcgcact gcccacactc ggcgcgttat
     4501 agaccggcgt atctttccag gtaactaagt tctgattact cagcggcttg ttgaggtcac
     4561 ccaaaccgtc ttcaactttt tgtcgcccct gaaccggctc gagatgccct ttatcgataa
     4621 accctttacc aaagccaaaa ttaaatgcgt aataacttgc ggcagtgggc tccacggcaa
     4681 tgatttgtcc ggcccgaata ttccccacgg tcaccatctt gtcgccaatt tgcgcccaga
     4741 ccgcagcatt ttcgatggtt tgcatataac gggcaggtaa cgcggcactg acacttgcgg
     4801 taaacagcat cagcaggaga ataacagctt gtttgtacat gaaaaaataa ccgagggtga
     4861 aacaacgctt ttattgtagc aaaagcgcta taagaatcgt gggttaatac tcatacaaat
     4921 tatgtttgag gacgaaaggc ggattttttt gttgttggtg ccaaagacta ctcacctccg
     4981 gcccacccag ttcgacaata cgctcgcgaa actctgaact ggtcatggtt tcacggcaag
     5041 ccatcatctg ttcaattaac ggtaacgtga caccagaaat cacttcgcaa cgggagtgtt
     5101 tgtggcttaa taatgaagcc acgcggtacg gtggcgctcc ggctatatcg gttaagaaga
     5161 tgacaccctc accagagtcc gcttcatgca gagcatcgca catcatgcgg ctgagcatat
     5221 tagagcttaa cccacgccag aaattcacgg cccggcactg aagaagcgcc ccatgttttt
     5281 tctccagcgc atccagtatc tcttgcgccc ggtcatcgtg acaggtaatt acccaaccta
     5341 acatcgcctt cctccttagc cgaaaatcag tctaccctga ccattgttga taaagggtga
     5401 tagccgtcaa aactcctctc ccccaaatcc agaggagagg aaatagcctt agctacgcaa
     5461 accacgtcca cgttggatca gcgaccaaca gatcaaataa aacgccacaa taaagaccac
     5521 cagtacgcca aaggtagtga ccagcggaac atcattgata ccgaggaagc cgtagcggaa
     5581 accactgatc atataaacga ttgggttcag gtgcgacagc ccttgccaga acggcggcaa
     5641 caaagtcagt gagtaaaaga ccccacccaa atacgtgagt ggcgttaaca caaaggttgg
     5701 caccaggctg atgtcatcga acgttttggc aaacacaccg ttcagcaaac ccgcaaggga
     5761 gaacaacacc gccgtgagca ccagggttaa ggcaacgaat acccacgaat gcacctgaaa
     5821 cggcacaaaa aacagtgaaa ttgccgtcac cagaatgcca acaaacagac cacgcgccac
     5881 gccaccgccg acatatccgg caataatgac gtgagtcgga accggcgcta ccagcagctc
     5941 ttcaatatta cgctggaact tggcaccaaa aaatgatgac gcaacgttgg cgtaggcatt
     6001 ggtgatcacc gacatcatga tcagccccgg tacgatgaac tgcatatagc tgaagccatg
     6061 catatcgcca atacgcgaac caatcaggtt accgaagata ataaagtaaa gggtcatggt
     6121 gatgactggc ggcaccagcg tctgcaccca gatacgcata aagcgatgga tctctttcgc
     6181 ccagatgctt tttagcgcca cccagtaaag atgcatcatg cgcgatctcc ttgtttttca
     6241 ttaaccagtg aaacaaacag ctcttccaga cggttagctt tgttacgcat acttaatacc
     6301 tgaatgccct gctcacttaa ctgcgtaaat acgctgttga tcccctgctc acgcagcact
     6361 tcaacttcca gcgtcgcggt atcgaccagt cgatactgat agccatcgag cttcggtaac
     6421 ggacttttcg gtgcgagatc gagaataaag gtttccgatt tcagcttcgc cagcagcgcc
     6481 ttcatcgagg tattttccac cagctcaccg tgttgaataa tgccgatatt gcggcacagc
     6541 atttctgctt cttccaggta gtgtgtggtg agaatgatgg tggtgccttt gtcgtttaaa
     6601 tccttcaaaa agccccacat tgagcggcga agttcaatat ccacgcctgc ggtcggttcg
     6661 tcgagaatca gtagtttagg ttcatgcatt aacgcacggg caatcattaa acggcgcttc
     6721 atcccgccag ataacatacg cgcacgttcg ttgcgttttc cccatagatc gagttgttta
     6781 agatattttt cgctgcggat gtacgcctct ttgcgctcca caccgtagta ccctgcctga
     6841 ttcaccacaa tttgctgcac ggtttcaaac gggttgaagt taaattcctg cggcaccagt
     6901 cccaactgac gtttagcgtt cacgacatct ttctcgagat cgtaaccaaa tacgctgacc
     6961 cgcccggagg ttttatttac cagagagctg ataataccga tagtggtcga tttcccggcc
     7021 ccgttcggcc cgagaagcgc ataaaaatca cccgcttcga cctgcaaatc tattccacga
     7081 agcgcctgaa cgccgcctgg ataggttttt ttaagctgtt gaagttccag tgcaatggtc
     7141 ataaattttt acttacctta cgttcttaca ctttatctgt ggtttaaatc gtcccggagt
     7201 tgccctatat tagccaaacg taattatttg gttacaggtc gttaacctcc atgaaagaca
     7261 tagatacact catcagcaac aatgcactat ggtcaaaaat gctggtggaa gaggatcccg
     7321 ggttttttga gaaactggca caagcgcaaa aaccgcgctt tctatggatt ggatgttccg
     7381 acagtcgcgt tcctgcagaa cgtttaaccg gtcttgagcc gggcgaactc tttgttcacc
     7441 gtaatgttgc taacctggtc attcacaccg acctgaactg cctttccgtg gttcagtatg
     7501 cagtggatgt actcgaagtt gaacacatta ttatctgtgg ccactacggt tgcggcggcg
     7561 tacaagccgc cgttgaaaac ccggaactgg ggcttatcaa caactggctg ctgcatatcc
     7621 gcgatatctg gttcaaacat agctcattgc tcggcgaaat gccgcaagag cgccgtctgg
     7681 ataccttgtg tgaactgaac gtcatggaac aggtttataa cctaggccac tccaccatta
     7741 tgcaatcagc gtggaaacgc gggcagaaag ttaccattca cggctgggcc tacggcattc
     7801 acgacggctt gctgcgtgat ctggatgtca cagcgaccaa ccgcgaaacc cttgagcaac
     7861 gttaccgtca cgggatttcc aacctcaagc tgaaacacgc caaccacaaa taaaaatgcc
     7921 atgccggatg caacacatcc ggcaacttca cacttactcg tcccgcagaa tcactttgcc
     7981 gatatacggc agatgacggt aacgctgtgc gtaatcaatg ccgtaaccca ccacaaactc
     8041 atccgggatc gagaaaccga taaattctac cgggacgttc acttcacgac gggacggttt
     8101 atccagcagc gtacaaatcg ccagcgactt cggttcgcgc aggcttaaga tctcacgcac
     8161 tttcgacagt gtattccccg agtcgatgat atcttcaaca atcagcacgt ccttgccacg
     8221 gatatcttca tccagatctt tgaggatttt cacatcacgg gtggtggaca tgccgctacc
     8281 gtagctggag gcggtcataa agtcgacttc atgagatacc tgaacttcac ggcacaggtc
     8341 cgccataaac ataaatgagc cacgcagcag acccaccagc accatatcgc tgccgctgtc
     8401 tttgtaacgc tcagtaatct gacgacccag ttcggcgata cgcgctttaa tctccgcttc
     8461 ggggatcatt acttctacag tatgtttcat atctctaacc atatgattta aaaacaaatc
     8521 acttcgcacg ggcaggtttt ggcaccgcaa caaagcgcaa gacacgcagt ataccagtaa
     8581 aactattcat tcgcggaatc tgttaataaa gctcgaattg tgatgacggt cacacatgtt
     8641 aaaacctata attaattgct actaaaatat taatgaattg aaatggtgtc tctttatggc
     8701 aattaacaat acaggctcgc gacgattact cgtcacgcta acagcccttt ttgcagcgct
     8761 ttgcgggctg tatctactca ttggcggagg ctggctggtc gcgattggcg gctcctggta
     8821 ctaccctatc gctggccttg tgatgctcgg cgtcgcctgg atgctgtggt gcagtaaacg
     8881 cgccgcgctt tggctatacg cagccctgct gctcggcacc atgatttggg gcgtctggga
     8941 agttggtttc gacttctggg cgctgactcc gcgcagcgat attctggtct tcttcggcat
     9001 ctggctgatc ctgccgtttg tctggcgtcg cctggtcatt cctgccagcg gcgcagttgc
     9061 cgcactggtg gtggcactgc tgattagcgg tagtatcctg acctgggccg gatttaacga
     9121 tccgcaggaa atcaacggca ccttaagcgc cgatgccaca cctgcggaag ctatctcacc
     9181 cgttgccgat caggactggc ctgcctatgg tcgcaatcag gaaggtcaac gcttttcacc
     9241 tctgaaacaa attaacgccg ataacgtcca taatctgaaa gaagcctggg tgttccgtac
     9301 tggcgatgtg aagcagccga acgatccggg cgaaatcacc aatgaagtga cgccgattaa
     9361 agtgggcgac accctttacc tgtgtaccgc tcaccagcgc ctgtttgcgc tcgatgccgc
     9421 cagcggcaaa gagaaatggc attacgatcc tgagctgaaa actaacgagt ctttccagca
     9481 cgtaacctgc cgtggcgtct cttatcatga agccaaagca gaaacggctt cgccggaagt
     9541 gatggcggat tgcccgcgtc gtatcattct tccggtcaat gatggtcgac tgattgcgat
     9601 taacgctgaa aacggcaaac tgtgcgaaac cttcgccaat aaaggcgtgc tcaatctgca
     9661 aagcaatatg ccagacacca aaccgggtct gtatgaaccg acttcgccac cgattatcac
     9721 cgataaaacc atcgtgatgg ccggttcagt taccgataac ttctcaaccc gcgaaacgtc
     9781 tggcgtgatc cgtggttttg atgtcaacac cggggagctg ctgtgggctt ttgatcccgg
     9841 cgcgaaagat ccgaacgcaa tcccgtctga cgaacacacc tttaccttta actcgccaaa
     9901 ctcctgggca ccagcggcct atgacgcgaa gctggatctg gtctatctgc cgatgggcgt
     9961 gaccacgccg gatatctggg gcggtaaccg cacaccggaa caggaacgtt atgccagctc
    10021 gattctggcg ctgaatgcca ctaccgggaa actggcgtgg agctaccaga ccgttcacca
    10081 cgacctgtgg gacatggatc ttccggcaca gccgacgctg gcggacatca ccgttaatgg
    10141 tcagaaagtg ccagttattt acgctccggc gaaaaccggc aacatttttg tgctcgatcg
    10201 tcgcaatggc gaactggtgg ttccggcccc ggaaaaaccg gtgccgcaag gtgctgcgaa
    10261 aggcgattac gttactccga cccagccgtt ctctgaactg agcttccgtc cgaagaaaga
    10321 tttgagcggt gcggatatgt ggggagccac catgtttgac caactggtgt gccgcgtgat
    10381 gttccaccag atgcgctatg aaggcatttt cacgccgcca tctgaacagg gcacgctggt
    10441 cttcccgggt aacctgggaa tgtttgaatg gggcgggatt tccgttgatc caaatcgtga
    10501 agtggcaatt gccaacccaa tggcactgcc gtttgtttcg aaactgatcc cacgcggtcc
    10561 gggtaacccg atggagcagc cgaaagatgc caaaggcacg ggtacggaat ccggcattca
    10621 gccacagtac ggtgtaccgt atggtgtcac gctcaacccg ttcctctcac cgtttggtct
    10681 gccgtgtaaa cagccagcat ggggttatat ctcggcgctg gatctgaaaa tcaatgaagt
    10741 ggtgtggaag aaacgtattg gtacgccgca agacagtatg ccgttcccga tgcctgtacc
    10801 ggtgccgttc aatatgggta tgccgatgct gggcgggcca atctccacgg cgggtaacgt
    10861 gctgtttatc gctgctacgg cagataacta cctgcgcgct tacaacatga gcaacggtga
    10921 aaaactgtgg cagggccgtt taccagcggg tggtcaggcg acgccaatga cctatgaagt
    10981 gaatggcaag cagtatgtgg tgatctccgc aggcggtcac ggttcatttg gtacgaagat
    11041 gggcgactat attgtggctt atgccctgcc ggatgatgtg aaataaccat aatgcccgga
    11101 gagatccggg catatttccg aatacggtct ttttataccg taaaccctaa catcatcccc
    11161 gtatcttcat gctccagcag atggcagtgc gccatataag catgttcttt cggtgcgttg
    11221 tgattaaact tcaccagcac ttcactgaca ttgccttcta ccttaacggt atctttccag
    11281 cccgcgcgat gagccgctgg cggtttgcca ttttctgaca agatacggaa ctgcatgccg
    11341 tgaatatgga acggatgcag catcatgtcg cccacgccag agataaccca acgttcgtac
    11401 tgccctttcg ccgccgcaaa catcggctta ttcatatcaa acgcctgacc gttgatttta
    11461 ttggcatggt ggaaatcgaa cttcccgccg tggttcatat gattcatatt gccgtgcccc
    11521 atatggccca tcatctggct gtgatccatt ccggccatcg cctgatcgcc atatttctcc
    11581 attagcatct gcatccccat catatcgagc atcgggtcca tagaaagttg cagcttgcgt
    11641 accgtcagcc cttccagcga aggtaacgca ggcaggctac ttaatgtgtc aggcaaagca
    11701 ccggaggcac taatagcaat cggctgaatc cgcattaccg gatgcggctt gtcaaacggc
    11761 gcaatcgcca tccccatctg gctgaccggt agcgtcacca gatcaaaggg tttgttgtcg
    11821 ttaacctcca ccagcacttc aaaacgctcg cccatcagca ccggcagctc gctcaccttc
    11881 accggttcag gtagcagacc accgtcgctg gcaatcacat acagcgggcg attgtcgctg
    11941 gtggcgaaat tgagcgagcg ggcgttacag ccattgagca aacgcaggcg cagccagcca
    12001 cgcggggcag cgtgttgtgg gtagattgca ccgttggtca gcagcgtatc gccaaaccag
    12061 cccacggcgg cggtcatcac atccagttga taatcaatct gcccatcagc gttaaatttt
    12121 ttatcctgaa caatcaccgg aacatcatcg ataccccact gttttggcag cattaatttc
    12181 aggatctcgt catcttcaat taccaccagc ccagccaacc ccatcgccac ctgtcgcccg
    12241 gtcttgccat gttgatgtgg atggaaccag caggtagcgg caggttgatc aacgttcaac
    12301 gtcaccgtac gtttgccgcc tggcggaata attccctgcg ggccgccgtc gacgtcaccc
    12361 ggtacttcca gcccgtgcca gtgcaacgtc gtctcttccg ccagttggtt atagatatca
    12421 accgttaccg ctttgccgcg ctgtaatttc accgccggcc ccagcagatt gccgttatag
    12481 ccccaggtag ttgcagtttt ctcgccaaag gtagactgac ctgcgccaat agttaactga
    12541 atgcgattac gggcatcggt cgtgagcaaa tcagggatcg gtaacgttgg gcgttccgcc
    12601 gcaaagactg cgcggctcca cagcggcaag gctgaagcca cacccagcgc gacggaatat
    12661 tttaagaaat cacgacgttg catagttatt tccttattct taagcaggcg aaacaaaatc
    12721 aaacgttgag catagtcctt ccccttacgg gaaggtcaag ccgcagacac aatttaatcg
    12781 acaataatgg tcgtcgcctc gctcgcagtg tgctaacgtt tatcttcttt aagcccctgg
    12841 tagaagcaat gaagacgttt ttcagaacag tgttattcgg cagcctgatg gccgtctgcg
    12901 caaacagtta cgcgctcagc gagtctgaag ccgaagatat ggccgattta acggcagttt
    12961 ttgtctttct gaagaacgat tgtggttacc agaacttacc taacgggcaa attcgtcgcg
    13021 cactggtctt tttcgcccag caaaaccagt gggatctcag taattacgac accttcgaca
    13081 tgaaagccct cggtgaagac agctaccgcg atctcagcgg cattggcatt cccgtcgcta
    13141 aaaagtgcaa agccctggcc cgcgattcct taagcctgct tgcctacgtc aaataatccc
    13201 tgataccttt ttgtagaaat attgaccgtg catccgcggt caatgttagc tattatgttg
    13261 cgcccttttt ttacgggtgt taacaaagga ggtatcaacc catggccgaa aaaaaacagt
    13321 ggcatgaaac gctacacgac cagtttgggc agtactttgc ggtagataac gttctgtatc
    13381 atgaaaagac cgatcaccaa gatctgatca tttttgagaa cgctgcattt ggtcgcgtaa
    13441 tggcgctgga tggcgtagta caaaccaccg agcgcgacga gtttatctat catgagatga
    13501 tgacccatgt tccgctactg gcccacggtc acgcgaaaca tgtgctgatt atcggcggcg
    13561 gcgacggtgc catgctgcgt gaagtaaccc gacataaaaa cgttgagtca atcacgatgg
    13621 tggaaatcga tgcgggtatc gtgtcgttct gccgtcagta tctgcccaac cataacgccg
    13681 gtagctacga cgatccgcgc tttaagctgg tgatcgacga tggcgtcaat ttcgttaatc
    13741 aaaccagcca gacctttgat gtcattatct ccgactgcac cgatcctatc ggtcccggcg
    13801 aaagtctgtt cacctcagca ttttatgaag gctgcaaacg ttgcctgaat cctggcggta
    13861 tcttcgtcgc acaaaacggc gtctgctttt tacagcagga agaagccatc gacagctatc
    13921 gcaaactcag ccattacttc agcgacgttg gcttttatca ggcggcgatc ccgacctatt
    13981 acggcggtat catgactttt gcatgggcga cagataacga cgccttacgc catctctcaa
    14041 ccgaaattat tcaggcgcgt tttctcgctt ctggcctgaa atgccgttat tacaatccgg
    14101 caatccatac ggcagctttt gccttacctc agtatctgca agacgcactg gcttcacagc
    14161 cgtcctaagg agaagataag aaattgaaaa aactgaaact gcatggcttt aataatctga
    14221 ccaaaagtct gagtttttgt atttacgata tctgctacgc caaaactgcc gaagagcgcg
    14281 acggttatat tgcttatatc gatgaactct ataatgccaa ccgtctgacc gaaatcctgt
    14341 cagaaacctg ttccattatc ggggccaata ttcttaacat cgcccgccag gattacgaac
    14401 cacagggtgc cagcgtcact attctggtga gtgaagaacc ggttgacccg aaactcatcg
    14461 acaaaacaga acaccccggc ccactgccag aaacggtcgt tgcccatctc gataaaagtc
    14521 atatttgcgt acatacctac ccggaaagtc atcctgaagg cggtttatgt accttccgcg
    14581 ccgatattga agtctctacc tgcggcgtga tttctccgct gaaggcgctg aattacctga
    14641 tccaccagct tgagtccgat atcgtaacca ttgattatcg cgtgcgcggt tttacccgcg
    14701 acattaacgg tatgaagcac tttatcgacc atgagattaa ttcgattcag aactttatgt
    14761 ctgacgatat gaaggcgctg tatgacatgg tggatgtgaa cgtctatcag gaaaatatct
    14821 tccataccaa gatgttgctt aaagagttcg accttaagca ctacatgttc cacaccaaac
    14881 cggaagattt aaccgacagc gagcgccagg aaattaccgc tgcgctgtgg aaagaaatgc
    14941 gcgagattta ttacgggcgc aatatgccag ctgtttaacg gctctggcgg agctcccagg
    15001 ctccgccaga tttatttact tctgctgcac gaaattgcgg taagccgcca cgacctgcag
    15061 aaaatcctca acgccgcata gcgacaggct ttcttcgtcg tagtagttca tcccttcttc
    15121 catttcatcg ccagcgaatt ccagttgatt ggcgcgaacc atcacctctt caccgtccat
    15181 ccacagggtg tattcatgcc ctgcccgttg ccaggaccgt tcgctacctt tcagtgcgtg
    15241 cgctgcttgt tccacttcat caagcaaagc caggttttct ttcacctctt cattaaacca
    15301 gtgcccgacc acttcatgcc ccatggacat acgcaccttt accactccgg taatatcgcg
    15361 cagaaattcg taatccatag tgtgttcctc tgctcccggc aatgcgccga aatcgcattg
    15421 cgctattgat gtaattattg cagccttacc ggagaaaaac agcggaacgg cgatggttta
    15481 gaaataaaaa atgcccggga atctcattca ccgggcattg tgtcgtttat gcgcagcgcg
    15541 tgcgcttact tattacaccg cagtctggaa aatcacccca tcggcttttt cggtgtactg
    15601 agaaagctgg ttgaagttca gataacgata agtatcaacg gcagttttat ctacttgcgc
    15661 aacgtaggtc tggtactctt ccggcgtcgg cagtttgcca atcagcgccg caacagccgc
    15721 cagttctgca gaagccagga agacattcgc gccagtcccc agacggttcg ggaagttacg
    15781 ggtagaggtg gaaaccaccg tcgcaccgtc tgctacacgc gcctggttac ccatacacag
    15841 agaacagccc gggatctcga tacgagcccc gctcttaccg aagacgctgt agtagccttc
    15901 ttcagtcaac tgtgcggcgt ccatgcgggt tggcggtgcc acccacaggc gggtcggcaa
    15961 ctggcctttg tgcgcatcca gcagtttacc cgcagcacgg aagtgaccga tgttagtcat
    16021 gcaagaaccg ataaacactt cgtcgatctt ctcgccctgt accgcagaca gcggacgtgc
    16081 gtcgtccggg tcgttcggtg cacagaggat tggctcttta atatccgcca gatcgatgtc
    16141 gatcactgcc gcgtattcag catctgcatc ggcttccagc agctcaggat tcgccagcca
    16201 tttttccatg ccctgaatac gacgttcgag ggtacgacga tcgccataac cttccgcgat
    16261 catccacttc agcaggacga tgttagagtt caggtattcg atgatcggtt ctttgttcag
    16321 cttgatagta caaccagcgg cagaacgctc agcggacgca tcggtcagtt caaatgcctg
    16381 ctcaattttc agatccggca gaccttcaat ttccagaatg cgaccagaga agatgttttt
    16441 cttgcctttc ttctcaacgg tcagcagacc ttgtttgatc gcatacagcg ggatcgcgtg
    16501 taccagatcg cgcagggtga tgcccggctg cattttgcct ttgaagcgca ccagaacgga
    16561 ttccggcata tccagcggca ttacgcctgt tgcggctgca aatgccacca gaccagaacc
    16621 cgccgggaaa gagataccga tcgggaaacg ggtatgggag tcaccaccgg taccgacggt
    16681 atccggcagc agcatacggt tcagccagga gtggattacg ccgtcacccg gacgcagcga
    16741 cacaccacca cggttcataa tgaagtccgg cagcgtgtgg tgcgtgttca cgtcaactgg
    16801 cttcggatac gccgcggtat ggcagaaaga ctgcatcacc aggtcagccg agaagcccag
    16861 gcacgccagg tctttcagtt catcacgggt catcgggccg gtggtgtcct gtgaacctac
    16921 agaagtcatt ttcggttcgc agtacgcgcc cggacgaata cctttcacac cgcaggcacg
    16981 acctaccatt ttctgagcca gcgagaagcc gcgatcgctc tcagcgacat ctttcgcctg
    17041 acggaacaca tcactgtgcg gcagaccaag tgcttcacgc gctttggtgg tcaggccacg
    17101 cccgataatc agcggaatac ggccaccagc acgcacttca tcaatcagca cgtcggtttt
    17161 cagttcgaag gtcgccagca gttcgccggt ttcgtggtta cgcacttcac ctttgtacgg
    17221 gtaaacgtca atcacgtcgc ccatgttcag gttagagacg tcgacttcga ttggcagtgc
    17281 acccgcgtct tccatcgtgt taaagaagat gggtgcaatt ttaccgccga ggcacaaacc
    17341 accgccgcgt ttgttcggca catgtggaat atcatcgccc ataaaccaca gaacggagtt
    17401 cgtggcggat ttacgcgatg aacccgtacc cacaacgtca ccgacgtacg ccagcgggaa
    17461 acctttctgt tgcagagctt cgatttgctt gatcgggcca acaacacctg gctggtctgg
    17521 ctcaatacct tcacgggcgt ttttcagcat cgccagcgcg tgcagtggga tatccgggcg
    17581 cgaccacgca tccggtgccg gagaaaggtc atcagtgttg gtttcgccag tgactttgaa
    17641 gacggtaacg gtcagttttt cagccagcgc cgggcgattc aggaaccatt cggcatccgc
    17701 ccaggactgc attacctgct tcgcatattc gttgcctgct ttggctttct cttctacgtc
    17761 atagaagtta tcgaacatca gcagtgtgtg agaaagtgct ttggcagcga tcggtgccag
    17821 tttggcatca tccagcgcgt cgatcagcgg atgaatgttg taaccaccct gcatggtacc
    17881 cagcagttcg atggcttttt ccggagtcag cagaggggat ttggcttcgc ctttagcgat
    17941 agcagccagg aagcctgctt tgacataggc ggcttcatcg acgccagggg gaacacggtt
    18001 ggttaataga tctaacagga attcttcttc gcccgcgggc gggtttttca gcagctctac
    18061 aagtgcggcc atttggtttg catccagggg tttgggcgca atcccctcag cggcacgctc
    18121 agctacgtgc ttacggtatt cttctagcac gacggttctc ctcgctctca ttgtcatagt
    18181 gcggcaggcg attctcttca cgctcctgtg agacagcagt ttgtagggta aatgcccagt
    18241 accgcaacgg gcagaatagc aggattttga cgacgtgtta atctgtttac aaaaaagcaa
    18301 cataaaaaag tggctgaatc gttaaggatg gtctagagat tgtttccctc cgtaaaaact
    18361 tcgttaattc gcatggtgat aatcaccttt ctcaacgaat cccaagatta gtcaaaattt
    18421 aaactaccgc ctctttatac tcggattaac agcacctgcg ggtggcagtt cgccgaccat
    18481 tgcgatttcc ttgagatccg aattatgaaa atgactttgc cgtttaaacc ccatgtgctg
    18541 gcactaattt gcagtgccgg gctttgtgcc gcctctgccg ggctatatat aaaaagccgc
    18601 acagtggaag cgcctgtgga aacgcaatcg acacaacaga ctgcgcctga catcaccgca
    18661 gttacgcttc ctgcaacggt ttccgcaccg cccgtaacgc ccgccgtcgt caaatccgca
    18721 ttcagcactg cacaaatcga tcaatgggtt gcgcctgtcg cgctgtaccc cgattctctg
    18781 ctttcacaag tgttaatggc atcaacctat ccggcaaacg ttgctcaagc agtgcaatgg
    18841 tcgcacgata atccacttaa acaaggcgat gctgctattc aggcggtatc tgaccagccg
    18901 tgggacgcca gcgttaaatc actggtggcc tttccacaat tgatggcatt gatgggcgaa
    18961 aacccgcaat gggtgcaaaa cctgggcgat gcttttctgg cgcagccgca ggacgtgatg
    19021 gactcggtac agcgattgcg gcaactggcg caacaaaccg gctcgctgaa gtcatcaacc
    19081 gaacagaaag ttattaccac aacgaagaaa gctgtaccgg taaaacagac agtcacggca
    19141 cccgtcatac catccaatac cgttttaact gccaaccccg tcattacaga gccggcaaca
    19201 accgtcattt ccattgagcc cgccaatcct gatgtggtct atattcccaa ctacaaccca
    19261 accgtggttt acgggaactg ggccaatact gcgtatccgc cggtttatct gccaccacca
    19321 gccggagaac cgtttgttga cagctttgtg cgcggattcg gctatagcat gggcgttgct
    19381 accacgtacg cactatttag cagcatcgac tgggatgacg acgatcatga ccatcatcat
    19441 catgacgatg atgattatca tcaccacgat ggcggtcatc gtgacggtaa tggctggcaa
    19501 cacaacggcg acaacatcaa tatcgacgtc aacaatttca accgtatcac cggtgagcat
    19561 cttactgata agaatatggc atggcggcac aatccaaact accgtaatgg tgtgccctat
    19621 catgatcagg atatggcaaa gcggtttcat caaaccgatg tcaacggcgg aatgagtgcc
    19681 acgcagctac ctgctccaac acgcgacagc cagcgtcagg cggcagcaag tcagtttcag
    19741 caacgaacac acgccgcccc cgtcattaca cgagataccc aacgtcaggc agcggcacag
    19801 cggtttaatg aagctgaaca ctatgggagc tatgacgact tccgcgactt cagccgtcgc
    19861 caaccactga cccagcaaca aaaggacgcc gctcgtcagc gttatcaatc ggcctctcct
    19921 gagcagcgcc aggcggttcg cgagaaaatg cagactaacc cacagaacca gcagcgaaga
    19981 gaggcagcgc gtgagcgtat tcagtctgca actcccgaac agcgccaggt gtttaaggaa
    20041 aaagtacagc agcgcccact gaaccaacag caacgtgata acgcccgcca gcgtatacaa
    20101 tcggcatcac ctgaacaacg tcaggttttt cgggaaaaag ttcaggagag ccgcccacaa
    20161 cgtctaaacg acagtaacca tactgtcagg ctgaataacg agcaacggtc agcagtacgc
    20221 gaacgtctct ctgagcgcgg agcaaggcga caggaaaggt aaattgcaga cgtaaaaaaa
    20281 gcggcgtggt tagccgcttt tttaattgcc ggatgttccg gcaaacgaaa aattacttct
    20341 tcttcgcttt cgggttcggc aggtcggtaa tgctaccttc gaacacttct gccgccaggc
    20401 ccacagactc gtgcagagtc gggtgcgcgt ggatggtcag tgcgatgtct tcagcatcac
    20461 aacccatttc gattgccaga ccgatttcac ccagcagctc accgccgtta gtaccgacaa
    20521 tcgcaccacc gatcacacgg tgagattctt tgtcgaaaat cagcttggtc ataccgtctg
    20581 cgcagtcgga agcgatagca cgaccagaag cagcccacgg gaaggtggcg gtttcatagc
    20641 tgatgccttt ctctttcgct tctttctcag tcagacctac ccatgcaact tctggttcgg
    20701 tataggcgat agacgggata actttcggat cgaagtagtg ttttttaccg gcgataactt
    20761 cagcggcaac gtgaccttcg tgaacacctt tgtgtgccag catcggctga ccaacgatat
    20821 cgccgatagc aaagatgtgc ggtacgttgg tacgcagctg tttgtcaacg cggatgaaac
    20881 cacggtcgtc cacttccacg ccagctttgc ctgcgtcgag gtttttaccg ttcggcacac
    20941 gaccaatcgc taccagcacg gcgtcgtaac gctgcggttc agcgggtgct tttttgcctt
    21001 ccatcgtcac ataaataccg tcttctttcg cttcaacggc ggtaactttg gtttccagca
    21061 tcaggttgaa tttcttgctg atacgcttgg tgaagacttt aacgatgtct ttgtcagctg
    21121 ccgggataac ctggtcgaac atttcaacca cgtcaatctg tgaacccagc gcgtggtata
    21181 cggtgcccat ttccagaccg atgataccgc cacccattac cagcaggcgt tctggtactt
    21241 ctttcagttc cagcgcgtca gtggagtccc agatacgcgg atcttcatgc ggaataaacg
    21301 gcagttggat cgggcgagaa cccgctgcaa tgatcgcatt gtcgaagttg atcacggttt
    21361 tgccgttctc accttcaact tccagggtgt tagccccggt gaatttaccc agaccgttga
    21421 ccactttgac tttgcggcct ttcgccatac cagccagacc accggtcagc tgattgatca
    21481 ctttctcttt ccaagtacga atcttgtcaa tatcggtttt cggttcgccg aagacgatac
    21541 cgtgttcagc cagcgctttg gcttcttcga taacttttgc tacgtgcagc agtgctttag
    21601 aagggataca gccgacgttc aggcaaacac cgccaagggt gttgtaacgt tctacgatta
    21661 cggtttccag acctaaatca gcgcaacgga aggcagcgga gtaacctgcg gggcctgccc
    21721 caagtaccac gacctgagtt ttgatttcag tactcatcat gacctctata tatttatctc
    21781 cggcggtcat acccgtcgtc tttcaggccg cagcggcgtt agcttcacgc cctcacccca
    21841 gtcacttact tatgtaagct cctggggatt ctggcgcttg ctaccttgct gcacccagaa
    21901 atccataggg tatgtgaagt aataccctaa ccaccaccgg gtcgttctat ccgtcggtat
    21961 tttacaaaat tgttaacaat ttttaaacaa caaacggcaa ccgatttgtc tattcgctaa
    22021 taacctcaat acattcatga gattaccaga aaaaagccgg ccgttgggcc ggctctttta
    22081 cttacatcac cagacggcga atgtcagaca gcgtgttgtt aatgatggta atgaaacggg
    22141 caccatcagc accgtcgatc acgcggtggt cgaaggagag agaaatcggc agcatcagac
    22201 gcggcacgaa ctctttacca ttccacaccg gctccatcgc ggacttggaa acgccgagga
    22261 tagccacttc cggcgcgttc acaatcggcg cgaagtgggt agtacccagg ccgccgatgc
    22321 tggagatggt gaagcaaccg ccctgcattt cgcccgcagt cagcttaccg tcacgcgctt
    22381 tcttagaaat agtcatcagc tcgcgagaca gctcgatgat gcctttcttg ttgacgtctt
    22441 tgaataccgg aacaaccaga ccgttcgggg tatccaccgc cacaccgatg ttgatgtatt
    22501 tcttcagggt cagacgctga ccgtcttccg acagcgaact attgaagcga ggcatctgct
    22561 caagagctgc agcaacggct ttcatgatga agacaaccgg ggtgagcttc acatccagct
    22621 tacgtttcgc cgcttcttcg ttctgctgtt tacggaacgc ttccagctcg gtgatatcgg
    22681 ttttgtcgaa gtgggtaaca tgcgggatca tcacccagtt acggctcagg ttagcaccag
    22741 aaattttctg gatacggccc agttccactt cttcgatttc gccaaacttg ctgaagtcca
    22801 ccttcggcca cggcagcatg ccagggatac caccgccagt cgccgccgga gctgcttctg
    22861 cacgtttgat agcttctttc acgtaagcct gaacgtcttc gcgcaggata cgacctttac
    22921 gaccagtgcc cttcactttc gccaggttaa cgccaaactc gcgtgccaga cggcggatca
    22981 gcggagtcgc gtgaacgtaa gcgtcgtttt cagcaaattc agatttgcct tcggctttcg
    23041 cagccggagc tgctgccggg gcttcagctt tcgctgccgg agccggcgct gccgcttcct
    23101 gtttcgcagg agctgccgca ggcgctgcgc cttcaacttc gaagatcata atcagcgagc
    23161 cagttttcac tttatcgcca acgttgactt tcagttcctt cacgacgcct gcaaacggag
    23221 ccgggacctc catagaagct ttatcgcctt ctacggtgat aagcgattgt tcagcggcaa
    23281 ctttgtcgcc cactttcacc atcacttcgg tcacttcaac ttcgtcaccg ccgatatccg
    23341 gaacgttaac ttctttcacg ccagccgctg atgcaggggc cgctgccgga gctgcttcct
    23401 gtttagccgc cggagctgcc gcgcctgctt cacccgcgac ttcgaagacc ataatcagcg
    23461 agccggtaga cactttgtca cccacgttca ctttgatctc tttcacggtg ccagcaaacg
    23521 gagccggaac ttccatagaa gctttatcgc cttctacggt gatcagcgac tgttcagctt
    23581 caactttatc gccaactttc accaggattt cggtcacttc aacttcgtcg ctgccgatat
    23641 ccggaacgtt aacgtctttt gccgccgcag ccgcgggtgc tgctgccgga gctgcttctt
    23701 tcttctcttc tgcctgagca ggtgcagcgt ctgctgcacc gtcggcggaa tcgaaaatca
    23761 taatcagtgc gccggtctgg gttttatcgc caacagagac tttgatctct ttaacgatac
    23821 ccgcctgcgg agacggaact tccatagagg ctttgtcgcc ttctacggtg atcagcgact
    23881 gttcggcttc aactttgtcg cccactttga ccaggatctc ggtgatttca acttcatcag
    23941 ccccgatgtc cggtactttg atttcgatag ccattattct tttacctctt acgccagacg
    24001 cgggttaact ttatctgcat cgatgttgaa tttggcgatt gcgtcagcaa ccactttctt
    24061 atcgatttcg ccacgtttag ccagttcgcc cagcgccgca accacgacat aagaagcatc
    24121 aacttcgaag tggtgacgca ggttctcacg gctgtcggaa cgaccgaagc catcagtacc
    24181 cagtacgcgg tagtcgtcag ccggtacgta agtacggacc tgctcagcga acagtttcat
    24241 atagtcggta gatgccactg ccggagcgtc gttcatcacc tgagcgatat acggaacgcg
    24301 cggagtttcc agcggatgaa gcatgttcca gcgttcacaa tcctgaccat cacgcgccag
    24361 ctcggtgaag gaggtcacgc tataaacgtc agaacctacg ccgtaatctt tcgccaggat
    24421 ctctgccgct tcacggacgt gacgcaggat agaaccggag cccagcagct gaactttacc
    24481 tttgctacct tcaatggttt cgagtttgta gataccttta cggatacctt cctcagcacc
    24541 ttccggcatt gccggcatgt ggtagttttc gttcagcgta gtgatgtagt agtaaacgtt
    24601 ctcttgtttt tcaccgtaca tacgctccag accgtcatgc atgatgacag caacttcgta
    24661 agcgtaagcc gggtcgtaag agatacagtt cgggatagtc agcgactgaa tgtggctgtg
    24721 accatcttcg tgctgcagac cttcaccgtt cagggtggta cgaccggaag taccgccgat
    24781 caggaagcca cgcgcttgct ggtcgccagc cgcccagcac agatcgccga tacgctggaa
    24841 gccgaacatc gagtaataga tgtagaacgg aatcatcggc agattgttgg tgctgtaaga
    24901 ggtcgccgct gccagccagg aacaccctgc gcccagctcg ttgatccctt cctgcagaat
    24961 ctgacctttc tcgtcttctt tatagtaagc aacctgctcg cggtcctgcg ggatgtactg
    25021 ctgaccgttc gggctgtaaa taccaatctg acggaacaga ccttccatac cgaaagtacg
    25081 cgcttcgtcg gcgatgatcg gtaccagacg atctttgatc gacttgttct tcagcattac
    25141 gttcagggca cgaacgaaag cgatagtggt agagatctct ttgctctgct cttccagcag
    25201 cgcgccgaag tcttgcaggc tcggcagctc aagcttctcg gtgaagttcg gctgacggct
    25261 tggcagataa ccgtgcagtt tctgacgctg tgcgtgcaga taggtatgct cttcagaacc
    25321 ttccgggaag gtgatgtacg gcagtttttc gatatctgca tcagacaccg gcacattgaa
    25381 acggtcgcgg atgtgacgca cgccgtccat gttcattttc ttaacctggt gcgcgatgtt
    25441 tttaccttca gccgcgtcgc ccatgccgta acctttaatg gtatgagcaa ggattactgt
    25501 cgctttgcct ttggtttcct gcgctttctt gaatgcagcg tagattttct tcggatcgtg
    25561 accaccacgg ttcagtgccc agatctgctc gtcagtccag tctgcaacca gtgctgcggt
    25621 ttcaggatat ttaccgaaga agtgttcacg aacgtacgca ccatctttcg atttgaaggt
    25681 ctggtagtcg ccgtcaacgg tttcgttcat cagctggatc agtttaccgc tggtatcttt
    25741 acgcagcagt tcatcccaac ggctacccca catcactttg ataacgttcc agccagcacc
    25801 ttcgaagatg ccttccagtt cgttgatgat cttgccgtta ccggtgaccg ggccgtcaag
    25861 acgctgcagg ttacagttga taacgaagac caggttatcc agtttttcac gggtagcgat
    25921 ggtgatcgca cctttggatt ccggttcgtc catttcaccg tcgccgagga acgcgtaaac
    25981 ggtctgttta gaggtatctt tcaggccacg gtgttccaga tatttcagga atttagcctg
    26041 gtaaatagca ccaatcggcc ccagacccat agatacggtc gggaactgcc agaattccgg
    26101 catcagtttc gggtgcggat aggaagagag gccattgccg tgaacttcct gacggaagtt
    26161 atccagctgc tcttgagtca gacgaccttc caggaaagca cgagcgtaca cgcccgggga
    26221 gatgtggccc tggaagtaaa ccaggtcgcc gccatcctgc tcgttgcgtg cacggaagaa
    26281 gtggttaaag cacacatcat aaatggttgc ggaagactgg aaggacgcca tatggccgcc
    26341 cagttcgagg tcttttttcg acgcacgcag caccgtcatg atggcgttcc agcggatagc
    26401 tgaacgaata cggcgttcca gttccagatt acccggatac tccggttgtt cttcaacggg
    26461 gatggtgttg atgtagttgc tgatacctgt gcctgcggct acgtttacac cgcctttgcg
    26521 ggcttcagca agcagttggt cgatcagata ctgagcacgc tcaacacctt cttcacggat
    26581 gaccgattcg atcgcctgga gccagtcgcg agtttcgatc ggatccacgt catttgggaa
    26641 acgttctgac atgggttatt ccttatctat ctaataacgt tgagttttct ggaacctgtc
    26701 ccattgaact ctcgccggaa agctcaataa gacaggttct acgtttagtt gccgcgcttt
    26761 tatatacgct tgatttacaa catcttctgg ataattttta ccagaaaaat cactaattct
    26821 ttcgttgctc cagacgacgc agagaacgct cacggcggct ctcttcacga cttctgtcga
    26881 gcaaaatttc ttcgataaag gccagatggc gatgcgatgc ttcgcgcgct tcttccggct
    26941 taccggccat aatcgcttca aatatgcggg tgcggtgact actcaccagc ggcagcatct
    27001 cgcgacgcga atagagcaat tcgaagttct ggcggacatt ctgggccaac atcggctcca
    27061 tacaccttag cagatgaagc agaaccacat tgtgggccgc ttcggtgacg gcaatctgat
    27121 actggagtac ggcgtttgat tccgcgtcca gatcgccaga ctgctgcgcc agctctatgg
    27181 cgtggtggag ttcacggatg cgttccttgt cttcatcggt actacgcagc gcggcgtaat
    27241 aagcggcgat accttccagg gcgtgtcgtg tttcgagcaa gtcatactgt gattcaggat
    27301 ggtcggagag cagctccacc agcggatcgc tgaagctttg ccataggctg ctctggacaa
    27361 aagtgccgcc accctggcga cgaagcaaca agcccttcgc ttcgagacgt tgaatcgcct
    27421 cacgcaagga gggacgggag acgtcgaact gttttgccag ttcgcgttcc ggtgggagtt
    27481 tttcgcccgg gcggagagtg ccttcgagaa tcaaaaactc cagttgctgc tcaatcacat
    27541 cggagagttt tggttggcgg attttgctgt aggccatgag ttcctgtctt aagccacttg
    27601 ccgaagtcaa ttggtcttac caatttcatg tctgtgacgc taaagtaaca aagtattcac
    27661 cttatgtcca tacaggtttt gattgaaatc atgaaactgt gcacatttta acaacttgac
    27721 atatataacg tttcaaaatt gtaactatgc acaaatgtag actttacgta ggaaagggat
    27781 tttttaacga ttattaaact ataaaaatcc gaattgaacc gattcactta ccaatttttt
    27841 gatttttaag tcaataaaaa cgaatttaaa ttcattctgc atattgagag gggttgaggc
    27901 tgagctttac aaacggtttc tttttaagca actcatcttc aaccatgcat aaagcgggtg
    27961 cattcgctgc cgcataccat tattcttgat ctgacggaag tctttttgta acaattcaaa
    28021 cttctttgat gtaaaaaaat taatacaaca aacggaattg caaacttaca cacgcatcac
    28081 tgcgtagatc aaaaaaacaa tcaccgcacg aggtttcatg atggaaggtc aacagcacgg
    28141 cgagcagcta aagcgcggcc ttaaaaaccg ccatattcag cttatcgcgc tgggtggcgc
    28201 gatagggaca gggctattcc tgggtagcgc ctccgtaata cagtccgcag ggccagggat
    28261 tatcctgggt tacgccattg ctggttttat cgcctttctg atcatgcgtc agctgggtga
    28321 aatggtggtc gaagaacctg tcgcaggctc ctttagccac tttgcttata aatactgggg
    28381 cagttttgcc ggtttcgcct ctggctggaa ctactgggta ctgtacgttt tagttgccat
    28441 ggcagagctg actgccgtgg gtaaatacat tcagttctgg tatccggaaa tcccaacctg
    28501 ggtttctgcc gccgtattct ttgtggtgat taacgccatc aacctgacca acgttaaagt
    28561 gtttggcgag atggagttct ggtttgccat tatcaaagtt attgcgatag tagcgatgat
    28621 catcttcggc ggctggctgc tgttcagtgg caacggcggc ccgcaggcga gcgttagcaa
    28681 cctgtgggat caaggtggtt tcctgccgca cggcttcacc ggactggtga tgatgatggc
    28741 gattatcatg ttctcgttcg gtggtctgga actggtgggg atcaccgcag cagaagctga
    28801 taacccggag caaagtatcc cgaaagcgac taaccaggtt atctaccgca tcctgatttt
    28861 ctatattggt tcgttagccg ttctgctctc actgatgccg tggacccgcg ttaccgccga
    28921 taccagtccg tttgtgctga tcttccacga gttaggcgat acctttgtgg cgaatgcgct
    28981 gaacatcgtg gtactgactg cggcgctctc cgtgtacaac agctgcgtat attgcaacag
    29041 ccgtatgctg tttggtctgg cacaacaggg taatgcgcca aaagcgctgg cgtctgtcga
    29101 taaacgcggc gtaccggtta acaccattct ggtgtctgcg ctggttacag cattgtgcgt
    29161 attgattaac tatcttgctc cggaatccgc atttggcctg ttaatggcac tggtggtatc
    29221 cgcactggtg atcaactggg cgatgatcag tctggcgcat atgaagttcc gtcgcgccaa
    29281 gcaggaacaa ggcgtggtaa ctcgcttccc tgctctgctt tatccgctgg gtaactggat
    29341 ctgcctgctg tttatggcgg cggtactggt gattatgctg atgaccccag gaatggcgat
    29401 ttcggtatac ctgatcccgg tatggctgat cgtgttaggt atcggctatc tgtttaaaga
    29461 gaaaaccgcc aaagccgtaa aagcgcatta atctctctac gccctcaccc atgcagggtg
    29521 agggcaataa tctttacacc aacgctccgt aaatcgtcag tagcgcaatc accaccacga
    29581 ccacgaacga ggttttcttc gccattgaaa ccgctgcctt cggcgtctcc accttatcga
    29641 catgcggttc acgcgccaga gagaactgcg ccagacgcgt taacacctga tactgcgaag
    29701 tatggaagtc acccagcgaa gcaaaccagg ccggtaacgc tttctcccca tgaccgatca
    29761 aggcatatac cacacccgca agacgaaccg gcacccaatc cagtacatga agcacggcat
    29821 caatgccgga ctgtaaacga tgatgcggcg tctggtatcg cgccagccag tattgccatg
    29881 cacgcaaaaa cgcatacccc atcagcgtaa cgggtcccca ggttcccccc acaatcaacc
    29941 agaacagcgg tgcaagataa aaacgaaagt taatccacag caatgcattt tgcagctcac
    30001 gcaaatactc acgttcgtcg cagcctgccg ggacgccgtg aatcatggtg agttcgccag
    30061 ccatcgtggc acgggcatgg ctatcattac gtgaagcagc tgtcagataa gcatgataat
    30121 gaagacgaac tttacctgcg ccaatacaca gcaaaccaat cagcagccac accagcagcg
    30181 tgggaacgtt gaacaatact ccctgcaatg cgcgtaacag taaaaaagtc acgcccatcg
    30241 caataatggt catgcctaac gtgcgcccga gagaaaaatg tttcacccgc cgaaagaacg
    30301 cttcaagacg atgatcaagc tgccagtgct cgcccaactt aaacaggcgc tcgaaaatta
    30361 acaccagtaa ggttgtaaat agcgtcatgt tgtctccttg ctgaccagcg cacgaaaccg
    30421 tgcccaatca aatgcaggac cgggatcggt tttccgatcc ggcgcaatat cacaatggcc
    30481 cgtcatgtta ttagcgatat cgggatagcg atctatcagt gcccgcgtaa ccgccgcaag
    30541 ctgttgatac tgcgcatcgg tatacgccag agtatcggtg ccttcaagct caatcccaat
    30601 agaaaaatca ttgcagcgtt cacgcccctg atactgagag actcccgcat gccaggcacg
    30661 tttatcgaaa ggaacatact ggactatttc accatcacgg cgaatcaaac agtgagcgga
    30721 gacgcgcaaa tgggcgatct cagcaaagaa aggatgtgcc tgcggatcaa tagttccagt
    30781 gaataatgcg tcgatccacg gaccgccaaa ctcgcctggc ggcaggctaa tattgtgcac
    30841 caccagcagg gtgggtgttt cgtcatccgg gcggcaatcg taatgtggtg agggaacgcg
    30901 gcgcgcgcca accagccacc cctgttctaa caacatgcag gatctcctta tatgtggtgc
    30961 taatacccgg ttcagagtag catgtttcta ccttatgatt cgttagctat ctggagtttt
    31021 aacatgccgc ctcgccgcta taaccctgac acccgacgtg acgagctgct ggaacgcatt
    31081 aatctcgata tccccggcgc ggtggcccag gcgctgcggg aagatttagg cggaacagtc
    31141 gatgccaaca atgacattac ggcaaaactt ttaccggaaa actctcgctc tcatgccacg
    31201 gtgagcaccc gcgagaatgg cgtattttgc ggcaaacgct gggttgaaga ggtgtttatt
    31261 caactggcag gcgacgatgt caccataatc tggcatgtgg atgacggcga tgtcatcaac
    31321 gccaatcaac ccttgttcga acttgaaggc ccatcccgcg tgctgttaac gggcgaacgc
    31381 actgcgctta attttgtgca aaccctttca ggagttgcca gtaaggtacg ccactatgtc
    31441 gaattactgg aaggcaccaa cacgcagttg ttggatacgc gcaaaacctt acctggcctg
    31501 cgttcagctc tgaaatacgc ggtactttgc ggcggcggag cgaatcaccg tctggggctt
    31561 tctgatgcct tcctgatcaa agaaaatcat attattgcct ccggctcagt gcgccaggcc
    31621 gtcgaaaaag cgtcctggct gcacccggat gcgccagtag aagtcgaagt agagaatctg
    31681 gaagaacttg atgaagccct gaaagcagga gccgatatca tcatgctgga taacttcgaa
    31741 acagaacaga tgcgcgaagc cgtcaaacgc accaacggca aggcgctact ggaagtgtct
    31801 ggcaacgtca ctgacaaaac actgcgcgaa tttgccgaaa cgggcgtgga ctttatctcc
    31861 gtcggtgcgc taactaaaca cgtacaagca ctcgaccttt caatgcgttt tcgctaattg
    31921 ctgacagatt tatccgctcc atcgcgggcg gataatctcc ccttccccaa ctttcttcgt
    31981 aacgcctcgc aaatttcttg tcttttgctg caacgaaata aaaaacctcg gaagctgctt
    32041 tcccgtttcg ttttttgccc ctcgcttgtg ctgatggcgt ttgccaaagt agcgccaacc
    32101 aaatcaagga gcgaaacaga tggacaagca acgcggtttt acacttatcg aactgatggt
    32161 ggttattggc atcattgcca ttttaagcgc cattggtatt cccgcttatc aaaactacct
    32221 gcgcaaagct gcactcaccg acatgctaca aacctttgtg ccttaccgta cagccgtaga
    32281 gttgtgcgcg ctggaacatg gtggattaga tacctgcgac ggtggcagca atggcattcc
    32341 ctcgcctacc accacccgct atgtttcagc catgagtgtg gcaaaaggcg tggtgtcgct
    32401 gaccgggcaa gaaagtctca atggactaag cgtcgtcatg acaccgggtt gggataacgc
    32461 aaacggcgtc accggctggg cgcgcaactg caatattcaa agtgacagcg cattgcagca
    32521 agcctgcgaa gatgtcttcc gctttgatga cgccaactaa ggagcggcaa tgaatattcc
    32581 acagctcacc gccctgtgtc tgcgttatca gggagtcttg ctggatgcca gcgaagaggt
    32641 ggttcatgtt gcggtagtcg atgcaccttc gcatgagttg ctggacgcat tgcatttcgc
    32701 taccaccaaa cgtattgaga tcacctgctg gacgcgccaa caaatggaag gccacgccag
    32761 tcgcacacaa cagacattgc ccgtagctgt tcaggagaag catcagccca aagcagagtt
    32821 gctgactcga acgttacaat ctgcgctgga acaacgtgcg tctgatattc atatcgaacc
    32881 agcggacaat gcctaccgca tccgcttgcg tatcgacggc gtattgcatc ctttaccgga
    32941 tgtttcaccg gatgccggag tcgcattaac cgccagatta aaagtgctgg gaaacctgga
    33001 tattgcggaa catcgcctgc cgcaggacgg gcaattcact gtcgaactgg caggaaacgc
    33061 cgtctcattt cgtattgcga ccttaccatg tcggggtggt gaaaaggtgg tattaaggtt
    33121 gttacagcag gtgaaccagg cactggatgt taacacgctg ggaatgcagc cgttacaact
    33181 ggcggacttt gctcatgcct tgcaacaacc acagggactg gtgctggtaa ctggccctac
    33241 aggcagcggc aaaacggtca cgctttatag tgccctgcaa aagctgaata ccgctgacat
    33301 taatatttgt agcgtcgaag atccggttga gatccccata gccggactaa accagacgca
    33361 aatccatccg cgtgccgggc tcacctttca gggcgttttg cgtgcgttat tgcgccagga
    33421 tcctgacgtc atcatgatcg gagagatccg ctatggcttt aacggcagaa attgccatta
    33481 aagccgcgca aaccggtcac ctggtgttgt ctaccctaca cactaattcc acctgcgaaa
    33541 cgctggtacg tttacagcaa atgggagtcg cccgctggat gctctcatca gcgcttacgc
    33601 tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca ttgtcgccag cagcaagggg
    33661 agcccatcca tattccagtc aatgtatggc cgtcgccgct gccccactgg caggcacccg
    33721 gttgtgtaca ttgctaccac ggtttttatg gtcgtacggc cttatttgaa gttctgccca
    33781 taacgccggt cattcgtcag cttatttccg ctaataccga cgttgaatcg ctggaaacgc
    33841 acgcacgaca ggcgggtatg cgtacgcttt ttgaaaacgg ctgcctggcc gtggagcaag
    33901 gcttaaccac ctttgaagag ttaatccgcg tactggggat gccgcatggc gagtaagcaa
    33961 ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc aagatgggat gctatgagca
    34021 gagagccgtg ctttgctgct catggcacta cagcaacaga tggttacccc acttagcctg
    34081 aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata aaagcgcgga agtcattcat
    34141 caactggcga cgctactcaa agccgggtta acgctttctg aagggctggc actgctggcg
    34201 gaacagcatc ccagtaagca atggcaagcg ttgctgcaat cgctggcgca cgatctcgaa
    34261 cagggcattg ctttttccaa tgccttatta ccctggtcag aggtatttcc gccactctat
    34321 caggcgatga tccgcacggg tgaactgacc ggtaagctgg atgaatgctg ctttgaactg
    34381 gcgcgtcagc aaaaagccca gcgtcagttg accgacaaag tgaaatcagc gttacgttat
    34441 cccatcatca ttttagcgat ggcaatcatg gtggttgtgg caatgctgca ttttgttctg
    34501 ccggagtttg ccgctatcta taagaccttc aacaccccac taccggcact aacgcagggg
    34561 atcatgacgc tggcagactt tagtggcgaa tggagctggc tgctggtgtt gttcggcttt
    34621 ctgctggcga tagccaataa gttgctgatg cgccgaccga cctggcttat agtgcggcag
    34681 aaattgctgt tacgcatccc gattatgggt tcactgatgc ggggacaaaa actcacgcag
    34741 atctttacga ttctggcgct gacacaaagt gcaggcatta cttttttgca gggcgtagag
    34801 agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac ttctgacaca aatccagcac
    34861 gatatcagta acggtcatcc catctggctg gcgctaaaaa ataccgggga gtttagcccg
    34921 ctctgtttgc aattagtgag aacaggagag gcatccggct cgctggacct catgttagac
    34981 aacctcgccc atcatcatcg ggataacaca atggcgctgg cggataacct cgcagcctta
    35041 ctggaaccga cgttgctgat cataacggga ggaattatcg gtacgctggt ggtggcgatg
    35101 tatctgccaa ttttccattt aggcgatgcg atgagtggga tgggataacg ctggcgtgtt
    35161 gctccacacc agcgttggaa gattacaggt tgttgaagat gcggttttct tgttcctgca
    35221 cacggataaa tgtggtgcgc ttggtcagtt ctttcaggcg tgaagcccca acgtatgtac
    35281 aagctgaacg caggccgccc aaaatatcac gcgcggtatt ttcaaccggg cctcgcagcg
    35341 gcagcttaac ggttttacct tctgctgcgc gatattccgc aacgccgcca acgtgacgtt
    35401 tcatcgcaga ctcggagctc atgccgtaga acagcataaa tttctcgccg ttctcctcaa
    35461 cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc gccaagcatg acgaaatcgg
    35521 caccgccgcc aaaggctttt gcgacatcgc ccggcgtggt gcagccaccg tcgctgacga
    35581 tcattccgcc cagaccgtgc gccgcatcgg cacattcgat taccgcagaa agctgcggat
    35641 aaccgacgcc tgttttgacg cgagttgtac aaacagaacc tgggccaatg ccaactttaa
    35701 cgatatcagc accggagagg ataagctcct cacacatttc accagtcact acgttgccag
    35761 cacaaatggt tttggtcggc cacgcttcac gcgctttcgc aacgaactgc acgaagtgtt
    35821 cggaataacc attcgccaca tcaatacaaa cgaagtttaa tgccgggttc aggtcgagaa
    35881 tctgtttagt tttttcgaaa tccgcatcag acgtaccggt agaaaccatc acatgtttca
    35941 gcacatcagc ggaagaattg ttgataaacg cttgccactc ttcgacagaa tagtgtttat
    36001 gcacagcagt caaaatatca aaagaagcca gcgcagaggc catagaaaat gtacctacgg
    36061 tgtccatatt tgcggcgata atcggcacgc cggaccagct ctgacctgaa tgtttgaagg
    36121 tgaattgacg ttccagttca acatcggaac ggcttttaag agtggagcgt ttagggcgga
    36181 tgagaacgtc tttaaaacct aacttcagat cttcttcaat acgcatgtgc ggattcctgg
    36241 ggttaatggc tataagggta agagcgcaac tccagtgacg ctatcatacg cactaatcaa
    36301 tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca atcccataaa tttacctggt
    36361 aaatagtgag cgaacgtttc atcaatttcg ctttcgtgca ctcattttta acgttttgat
    36421 aatcatgatg aattcccggg aatataaatt atgaggtata tagttgcctt aacgggaggc
    36481 attggcagtg gcaagagtac cgttgccaat gcgtttgctg atctcggaat taacgtcatt
    36541 gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg cacctgcgct acatgccatt
    36601 gctgatcact ttggcgctaa catgattgct gctgatggaa cattgcagcg ccgggccttg
    36661 cgcgagcgga tcttcgccaa cccggaagag aaaaactggc ttaacgccct gctgcatccg
    36721 ctgattcagc aagagacgca acaccagatc cagcaagcta cttcccccta tgtactgtgg
    36781 gttgtgccat tgctggtaga aaactcactg tataaaaaag cgaatcgagt gctggtggtg
    36841 gatgtcagcc cagaaacgca acttaagcgc accatgcagc gcgatgatgt aactcgcgag
    36901 catgtcgaac aaatccttgc tgctcaggca acgcgcgaag cccgccttgc cgtggcagat
    36961 gacgtcattg ataataacgg cgcaccggat gctatcgcat cggatgttgc ccgcctgcac
    37021 gcacactatt tgcagcttgc gtcgcagttt gtctcacagg aaaaaccgta atgcagaccc
    37081 aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac atggctgcgc attgagtttt
    37141 tgattcagca actcaccgtt aatttaccca tcgttgacca cgctggcgcg ctgcatttct
    37201 tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg cgaagtccgc actgagctgt
    37261 tgaaagaact tgaccggcag caacgtaaac tccagacctg gattggcgtg cctggcgtgg
    37321 accagagccg tattgaagca ttaattcagc agttaaaagc ggcggggagc gtattaattt
    37381 ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt gattgctctg gtgcgtcagc
    37441 gactgagcat cccaggcggc tgttgcagct ttgatttacc tacattgcac atttggctgc
    37501 atctacccca ggcgcagcgc gacagccagg tagaaacctg gattgccagc ctgaacccgc
    37561 tcacccaggc acttaccatg gtgctggatt taattcgcca gtcggccccc ttccgtaaac
    37621 aaaccagtct gaatggtttt tatcaggata acggtggcga tgccgacttg ctgcgcctga
    37681 atctgtcgct cgattcacag ctttatccgc aaatttccgg acataagagc cgttttgcca
    37741 ttcgttttat gccgctggac actgaaaacg gacaggtacc ggaacgtctg gatttcgaac
    37801 tggcctgttg ctaaggagta aaaatgtcag aaactattac ggtgaattgc ccaacctgcg
    37861 ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc attttgctcc aaacgttgtc
    37921 agctgatcga cctcggagaa tgggctgctg aagaaaaacg aatccccagc agtggcgatc
    37981 tttccgaaag cgatgactgg agcgaagagc caaagcagtg acatttgtct gatgccgcac
    38041 gtaggcctga taagacgcgg acagcgtcgc atcaggcatc ttgtgcaaat gtcggatgcg
    38101 gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg caattaccgg ttcattggct
    38161 ggcggaaaat catcggcatt aagaccgact agcgacatcc actcaccggg ttgcccttct
    38221 ttaccccacg gctccccttc ccagctttcg accagccaga accacagagt tatatgcctg
    38281 tccgggaatt catattccag tttttcaaat agcgaaaaat gttgtggggt aatcccgact
    38341 tcttcctgaa gttcacgcac taccgcctgt tccggcgttt cgcccatttc aattttaccg
    38401 ccgggaaact ccagtttatt cgccatgtgc gcatctgctg cgcgacgcgt tataaagatt
    38461 tcattgttct cgttgcgaat aatacctacc gcaatttgca gctttttcat tgtcttaaac
    38521 ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac tgttagcttt tattgcaggc
    38581 gaccatggca ctgcttgtat tttttaccag aaccgcacgg gcaaggatcg ttacgtccta
    38641 ctttgcgctc accggtttgc gccgccagtg cagctgcggc tgcagagtcg tcatcctgat
    38701 ggctaagctg ctgcatttgc gctaaacgct cggcttccat acgacgctgt tgttccagct
    38761 cctcaacctc ttcaggcata cgtacctgga ctttgctcag cgtgctgata acttcatatt
    38821 ttaacgactc cagcatcgct gcaaacatgg agaacgattc acgtttgtat tcctgcttcg
    38881 gatctttctg tgcatagcca cgcaggtgga taccctgacg cagatagtcc atcgccgcta
    38941 ggtgctcttt ccacagagag tcgagagttt gcagcatgac gcctttttcg aagtgacgca
    39001 tcatctcagc accaaccact tcttctttac gctgatacac ttcgatggac tgcgccagaa
    39061 ttcgctcacg cagcgtctct tcatgcagtt ctggttcttt atccagccac tcggcaattg
    39121 gcaaatcgag gtcgaaatcg ttcttcagac gttcctgcag ccccggaata tcccacattt
    39181 cttccagtga ctgtggcgga atgtaggcat caatggtcgc tttgaacaca tcttcacgaa
    39241 tgctgttgat ggtttcgctc acatcgctga catccagcag ttcgttacgc tgggagtaaa
    39301 tggcgcgacg ctgatcgtta gccacgtcat catattccag cagttgctta cgaatgtcga
    39361 agttacggct ttcaacttta cgctgggcgt tggcaatcgc tttagtcacc cacgggtgtt
    39421 caatggcttc gcctggcttc atacccagtt tacgcatcat gccggatact cggtcggaag
    39481 caaaaatacg catcagcgca tcttccatcg acaggtagaa acgggaagaa ccagcatccc
    39541 cctgacgacc agaacgaccg cgcaactggt tatcgatacg acgggattcg tgacgctcgg
    39601 taccgatgat atgcaggcca cctgcttcca gtaccgcatc gtgacgtacc tgccagtcgg
    39661 ctttaatttt ttcaatttgc tctgcggtcg gattttccag cgcggcaact tctgcctgcc
    39721 agctaccacc gagcacaata tctgtaccac gacccgccat attggtcgcg atagtcaccg
    39781 cagccggata acctgcctga gcaacaatcg ccgcttcgtt ggcgtggaat ttggcgttca
    39841 ggacgttgtg cttaataccg gctttggtca gttcgtttga caccagctcc gatttttcga
    39901 tggagatagt acccaccagc accggctggc ctttcgcagt acgttctttg atatcttcaa
    39961 tgatcgcctg aattttttcc gcttcagtca tgtagaccag gtccggcaga tctttacgaa
    40021 tcattggacg gttggtcgga acaacgacgg tatccagctt gtagatggag ctaaattcga
    40081 aagcttcggt atcagcagta ccggtcatcc ccgccagttt ttcatacaga cggaagtagt
    40141 tctggaaggt gatcgaagcc agcgtttggt tttcgttctg gatctgcaca ccttctttcg
    40201 cttccacagc ctggtgcaga ccatcggacc agcgacggcc ctgcatggta cgaccggtgt
    40261 gttcgtcaac gatgataact tcaccatctt taacgatgta gtcgacgtcg cgggtaaaca
    40321 gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag catgatgttg gccggagagt
    40381 acagagactc cccttcatcc atgatgccct ctttcaccag cagttcttca atcagcacca
    40441 gaccacgttc ggtcaggttc acctggcgag atttttcgtc caccgagaag tggccttcgc
    40501 cctggaaggt ttcggagtct tctttttcct gacggatcag gtgcggaata attttattca
    40561 cgcgtttata catttccgag ctgtcttctg ccgggccgga aatgatcagc ggtgtacgcg
    40621 cttcatcgat caggatggag tccacttcgt ccaccagcgc atagtgcagt ttacgctgta
    40681 cacgttcttc agggctgaac gccatattgt cgcgcaggta gtcaaagccg tattcgttgt
    40741 tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt tgccggtgct ggcatgcccg
    40801 gcaggttgat accgacagtc aggccaagga attcaaacag cggacggttg ttttcggcgt
    40861 cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac gcctttaccg gttagtgcgt
    40921 tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc accggtacgc atttcggcga
    40981 tgcagcgttc gttaagaacc ataccgccga gtaactgaac gtcgaagtga cgcataccaa
    41041 agacgcgctt acttgcctca cgtaccacgg cgaaagcttc cgggatcaga ttttccagca
    41101 cttcgccttt ttccagacgt gcacgaaact ctgcggtttt ccctttcagt tcttcgtcgg
    41161 agagtttttc catctccggt tccatggcat tgatgatgtt gaccactttg cgcatccggc
    41221 gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa caatttgatt agcataataa
    41281 aatctcaaac gccccgcgtt gcggagttaa taaaatgaag taaaggttta ttgttgttag
    41341 gtgaggcgtt gagggccagc acggatgcct tgcgcctggc ttatccagac gggcgtgctg
    41401 aattttgctt gtggggtaaa atgcgcataa tcaatgcgat aacccttttc agacggcgtg
    41461 ccttcctggg tcagcagcgc gctgagcgta tccagtaatg caagatgttg cgcctgaaga
    41521 ggcaaagact cttcagcaac gggcagtgtt tgcggtgcca ttgcgaaaga aagatgacgg
    41581 attaccgtgc gaatggcatg ttgatgccag taatcaacgg aatagttcga attcgggcgg
    41641 cgtgtgttcg cttccagcaa ggccaattga ccaaagttaa ctttggctga aggctcgtgg
    41701 ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg cgttgctgag cgcaggcaaa
    41761 cctaaactcg ccgcaaccat ccctaataag agatgcggcc agaagtagcg tttaccaaac
    41821 tgtcgccagc gcgtcagtat tccactcacg ttattgccat cccgatcgta ttcaaacgta
    41881 tttagcgcac aaatcttatc attaaagcct gcagtccaca gcacgaatga agataagttg
    41941 tgcggaaaag tgcttaagaa aacagcgttc gcaccgtttt ttatagctct tataaaattc
    42001 aaaaaaaaat gactggttca cctggccgga gagagtgcca gatttgccag tcgaatttta
    42061 tacgacagta taaatgtcgt tatgccagta cagctgaagg cgctttgaag gccaacggca
    42121 gttctgcgtc gtcctggaag gtcacatatt cccaggcttc ctgtttcgcc aggacagcct
    42181 gcagcagttt gttattcagt gcatgaccgg atttataagc ggtaaatgca ccaataatat
    42241 tgtgaccaca catgaacaag tcaccgatcg catcgagcat tttgtgacgc acaaattcgt
    42301 cttcaaaacg caggccgtct tcgttcagta cgcgataatc gtcaacaacg atggcacaat
    42361 cgaagctgcc gcccaggcac aaaccacggg actgcagata ttcgatatca cgcatgaaac
    42421 cgaacgtacg cgcacggctg atctggcgca taaacgcatc agcggagaag ttcatcgcat
    42481 agcgctggtt gctggaatca atagccggat ggttaaaatc gatggtgaaa tccagcgaaa
    42541 aaccattgta cggcttaaat tcagcccact tatcgccatc ttcgacacga acagtctctt
    42601 tgatgcgaac aaattttttg gcgcagttca actcgtcgat accggcgtca agcagcaggt
    42661 atacaaacgg agcggcgctg ccgtccatga tcgggatttc cggcgcgtta acttcgataa
    42721 caatgttatc gatgcccagg cccgcgagag cagcattgag gtgctctacg gttgaaatcc
    42781 gtacatcatg ctcgttgacc agacacgtac agagcatggt atcacgcaca gatttggcat
    42841 cggccgggaa atctaccggt ggattcaagt cggtgcgacg atagatgacc ccggtgttgg
    42901 ccggcgcagg gcgtaacgtc agagtgactt tcttgccggt atgtaaaccg acacccgtcg
    42961 cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt attatctcgc caaattacct
    43021 atccaaccga agtgtactat acattcggcg ggccagttta gcacaaagag cctcgaaacc
    43081 caaattccag tcaattctta atcagcttgc ttacgcagga atgctgggat atccagataa
    43141 tccggctctt tcgcagtttg cggcgcattg tcattcacga ctttagcaac cggcttctgc
    43201 tcctgggtca gcggagccat cccatgctgc tggtagcgat ccatcactgg ctgctgaacc
    43261 tgcttattgg tcaccagagt gatttcagga cgtttgtcca tgccgatacc tgtcgcaaca
    43321 acggttacgc gcagctcgtc attcatatcc gggtcaagag aagtaccgat aaccacagtc
    43381 gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg tttcgaactc atccagacgc
    43441 aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg cgccagacag gtcgatatct
    43501 tccagcagcg gagaagagat agccatttca gcagcttctt ccgcacggtc ttcaccgctc
    43561 gccacgccag aacccatcat tgcgtagccc atctcagaca ttacggtgcg tacgtctgca
    43621 aagtccacgt tcatcaaacc cggacgagta atcagttcag cgataccttg cacagcgcct
    43681 ttcagtacat cgttcgctgc gccaaacgca tccagcaggg agataccgcg gcccagaact
    43741 ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca catgcttgga cagttcagtg
    43801 atcccctgct ccgcgaatgc catacgcttc ttgccttcaa agttgaaagg cttagtgacg
    43861 acagcaacgg tcaggatacc caaatctttt gccacttcag cgacgactgg tgctgcacct
    43921 gtaccggtac caccacccat acccgcagca ataaagacca tgtctgcacc ttccagcgcc
    43981 gcacgcaatg catcgcgatc ctcatcagcc gcattgcgac caacttctgg attagcgcca
    44041 gcgcccagtc ctttggtgat accgctaccg atttgaatcg tctgtccaac cgctgtttta
    44101 cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa caccttcaat acgctcgcgc
    44161 accatgtgtt caacagcatt accgccgccg ccgccgacgc cgatgacttt aatcaccgcg
    44221 tcattggtaa gttccattgg ttcaaacata gtttctctcc gatttgtgcc tgtcgcctga
    44281 ggccgtaatc atcgtcggcc tcataaaaat taaaactctt ttcgcagcca actattgagt
    44341 cgcttgatcc acgagccaac tgatgctgta acacgttttt ctacttcagc ttcaccgtta
    44401 agatgtgact ctttcccata gtgaagcaat cccaccgccg tcgaataata cggctcctga
    44461 gcataatccg ttaaaccggt aatgttcagc ggcgcgccga tacgcacttg cgtatgaaac
    44521 acgcgctgag cacaggctgc aagaccttcg atctgcgctg cgccaccggt taatacaatg
    44581 cctgccgcca ggtgatgttt aaccccttgt tggcgaagct tttcctgcaa ctgcaatatc
    44641 tcttcgttga ccaggttgag cagctcggta tagcgcggct cgatcacctc tgccagggtc
    44701 tgacgttgca gactccgtgg cggacgaccg cctacgctcg gcacttccac gctctcatct
    44761 tttccaacga tggaacccag cgcacaaccg tggcgaactt taatcgcttc ggcgtcgctt
    44821 ggcggcgtgc caaaggcgta agcgatatca ctggtcacga cattgccagc ataaggaatt
    44881 accttagtgt ggcgcaatgc cccaccggta taaacggcga tatccattgt accaccaccg
    44941 atatcgacga cgcagacacc cagttcacgt tcatcttccg tcaataccga ataacttgat
    45001 gccagtccgg caaatatcag ttggtcaact ttcagcccac aacgttcaac cgctttgacg
    45061 atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca cttttgcctg catccgcacg
    45121 cccgaaagtc ctaccggatt cttgatccct tcctgatagt caatcgcata ctcttgcggg
    45181 atcacatgca gcacacgatg ttcatcgcgc acacgcaccg atttcgcggt atggacgacg
    45241 ttttccacat cttcttgcgt cacttcttct tcagaaatag gcaccatacc aatttcattc
    45301 tggcagctga tgtgcttacc agaaagcgcc agatataccg aagagatctg acaatctgcc
    45361 atcaattctg cctggtcaat ggcgcgttgt acgcacttga ccacggattc gaggtcgttc
    45421 accccgcctt tatccatacc acgtgacggg cagctgccca cgccaatgat attgaccata
    45481 ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg cggtaccaat ctccagtcct
    45541 actaccagtt ttctgtccgt cgccttgatc attgttgttc tgcctgtgcc tgattttgtt
    45601 gctgagtaga ttcctctggc ggcaagggcg cccagcctac tgccgctcca gagtcataac
    45661 gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc ctgctgctgt aaaaccggat
    45721 aaagttctac aaagcgagcc aaacgtttca tcgtatcgcc ccggccaaga ttgagcttaa
    45781 tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt catcgccgct tccttcagag
    45841 taaatctgtc ctttgccagc atctgcccca tttcgcgata gccctgcaac acttcattgg
    45901 cgctgccttc cgggccatac agcattggaa gcacctgctt gctggtgcga tctggcggca
    45961 cgctgaaggt gtttccttcc gcgtctacca tatgttgatc attccaccgc gcaatcggca
    46021 catattcaac cagatgaatc ttcaattcat caggccactg ctttctgacg ctcacctgct
    46081 taatccacgg caggcgttgt tctatttgcg tctggatgat gttgacatcc tgggtcataa
    46141 aggtacccgg ctcacccaat gccaggatcg actgccggat atcgtcatta cgcgtgtaat
    46201 ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg ttgcgcatct tccatccagc
    46261 ccaacacgac ccagccgctc accaacactg tcgttaaaac ggtcagcagg aaaaggatcc
    46321 ccgccagacg cgttccatta ttgcggcgag aagaaacctc ttcttcgcta tttcgcgtgt
    46381 tcagagcagc ctgcgacata ttagtccgcc agttccagaa ttcgtactac caactgcgag
    46441 aagctcatac ctgcctgacg tgccgccatc ggcaccaggc tgtggctggt catacccggt
    46501 gaggtattgg cttccagcag ataaaactgt ccatcgctgt ccagcataac gtcaatacgt
    46561 ccccatcctt tgcaacctaa cgtcgtccat gctttcagca ctaatgcctg caaattggcc
    46621 tcttgtgacg cttccagacc tgcggggcag aaatactgtg tctcatcaga gagatacttc
    46681 gcctcataat catagaaggt tccggacggt tgaatacgta ttgacggtaa aatttcttca
    46741 ccgagtatcg caaccgtgaa ctccggccca cttagccatt tttcaatcaa tacttcttca
    46801 tcgtgctgaa atgccaatct taatgcatct tgtagagcat tttctgctac tacttttgac
    46861 attcccacac tggaaccttc gcggctcggc ttaacgataa ccggcaaacc cagagcagaa
    46921 atttctgcta actgcttatc gctcaggcct ttttcaaact ctgcgcgggt taacgctacc
    46981 cacggcgcga ccggtaaacc ggcaccttgc cacagcagtt tgctgcgtag tttatccatt
    47041 gaaagcgcag atgccatcac tccgctgccg gtataaggca agcccatcag ctcaagcatg
    47101 ccctgcagcg taccatcttc accgccgcga ccgtgtagcg cgataaacac tttctgaaaa
    47161 cccatcgact tcagttgcgt cacgtcgact tctttcgggt cgacaggata cgcatcaata
    47221 ccgccttcac gcagcccggc taacaccgct gcgccagaat tcagagaaac ttcccgctca
    47281 gcggaagtcc cgcccaacag gaccgcaatt ttatcagtca tgttgttctt cctccggagt
    47341 ttgcggcttc agtttgattt cagctaaaga acgggcaatt tttccgatat taccagcccc
    47401 ctgaacgaga atcaggtcgt taccgattaa taccggtgcc agcatctcgg ctacctgcgc
    47461 agggtccggc accagaatgg gatcaatttt cccacgtcca cgaattgtgc gacacagcga
    47521 acggctgtcc gctcccggaa ttggcgcttc gccagccgga tacacttcca gcatcaacag
    47581 ggtatcaacc tgcgtcagca cattggcgaa atcatcatac aggtcgcgcg tacgggtaaa
    47641 acggtgcggc tgaaacagca ttactaggtt tttatccggc cagcctgcgc gcgccgcttt
    47701 aatggtggcg tcaacttccg tcgggtggtg gccgtagtca tcgaccagca ttgccgtacc
    47761 gcttttacca ttcactggct ccagcgggaa ttcaccgagg aaatcaaaac ggcgaccagt
    47821 cccctggaag ctctccagcg cacgcaaaat agcctcgtcg tcaattccct cttccgtagc
    47881 aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg cctggcgcat tcagggtgac
    47941 gcgcatcggc tctttgtcct ggcgcagcag cgtaaagtgc ccctgcgggc caatctgctg
    48001 ataatcttct acacgcacgt cggcatcttc gctgaagccg taagtcgtgg tctgacgtcc
    48061 cacacgcggt aacaattcgc ggatcaccgg atcatcaaca cacatcaccg cacgaccgta
    48121 aaacggcagg ttgtgcagaa aattaataaa agtctgtttt aaattctcaa agtcgccctg
    48181 gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc gccaccatcg gttgcagatg
    48241 caggaacgat gcatcactct catctgcttc ggcaatcagg taccgaccat gccccaaacg
    48301 cgcatgaacc cccgccgctt ttaccagccc gccgttaacg aaggttgggt cgagccccgc
    48361 ttctgcgtag atgctggaaa ccatcgcggt ggttgtcgtt ttgccgtgcg ttcctgcaat
    48421 ggcgatgcca tgacgaaaac gcattaactc agccagcatt tcggcacgac ggatcaccgg
    48481 aatacgcgct tcatgagctg cgacaatttc cgggttatcg gcagaaatgg cgctggaaac
    48541 aacgaccacg ctggcatcac gtacgttttc cgggcgatgg ttgaaataaa tcgtcgctcc
    48601 cagattcatt aactgctgcg tgaccggatt tggcgctaaa tcggaaccac tgatctgata
    48661 accttcattg gccagaactt cggcaatacc gcccataccg gcaccaccga tgccgacaaa
    48721 atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa cgcagttttg ccaattgttg
    48781 tgtattcatt ctttacgcca ttaacttctt aaattcatac gatgcaaaag gcatcgctac
    48841 aattacgccc gggcagcccg gctcacttca tttgccactc gctcggtggc atccggaatg
    48901 gatgcagcgc gggcgcgttc tgccatggtt aataaggttt ctcgcgacca cccggccagg
    48961 gtgttggcga cagcatccac gctaagctgt ggctgctcga taattttggc tgcgcccgct
    49021 ttttccagcg gtagcgcatt ccagtattgc tggcggtctt tatgttgaaa cggcacaaac
    49081 aacgccggta gtcctgccgc ggcgatttca ctcaccgtta aagcaccgga gcggcaaacg
    49141 acgacatccg cccacgcata cgccgccgcc atatcatcaa taaattccgt cactttatgc
    49201 tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt gttgcgaacc tttaccgctc
    49261 tgatgccaga tagtgactga atcacccagt ttcgcagcaa cctgcggcat tgtctggtta
    49321 agaatgcgtg cgccctggga accacccact accagcacac gaaccggacc ttcacgtcca
    49381 gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg tacgcaccgg gttacccacc
    49441 acttccgcat tagggaaagc gcctggaaac gcctgcatca ctttggtggc aatcttcgcc
    49501 agccatttat tggttaagcc cgcaataccg ttttgttcat gaagtacaac cggaatgcct
    49561 aacgaccacg cggccagacc acctggacct gatacatagc cgcccatacc gagtaccacg
    49621 tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc aggcgttgaa gatacgcagc
    49681 ggcgcagcta tcagtgcttt tatacctttt ccacgcagac cagagatacg aatgaaatca
    49741 atttcgatgc catgttttgg cactaagtcc gcttccatac ggtcggcagt ccccagccag
    49801 cgaacttgcc aaccctgagc cattagatgg tgcgcaaccg ccagtcccgg gaatacatgt
    49861 ccaccggttc cgcctgccat caccattaat cgctttcctt gaccactcat cgtgaacctc
    49921 gtacaaacgc ctgcgctttc tccagacgcg tttcataatc aatacgcaac agcatcatga
    49981 tggctgtcga cataatcagt aagctcgaac caccgtaact gatcagcggc aatgtcagac
    50041 ctttggtcgg taacatcccc gccgccgcgc ctacgttaac cagcgcctgg aagctaaacc
    50101 agatgccaat agaacaggcg agaaaaccgg aaaaacggtg gtcaatttct aacgctttgc
    50161 ggccaatcga catcgcgcga aaagcgacga agaataccat taaaagtgcc agcaccacac
    50221 cgacataccc cagttcttcg ccgataatgg cgaaaataaa gtcagtgtgc gcttccggca
    50281 gatactccag tttttgtacc gagttaccta aaccttgtcc ccaaagttcg ccgcgaccaa
    50341 acgccatcag cgattgcgtt aactgatagc cgctgccaaa gggatcttcc cacgggttcc
    50401 agaatgcggt aacacggcgg atacggtacg gttcggcgag tatcagcaac acaaccgctg
    50461 aaatgcccat accgataatg gcaatgaact gccacaattt cgctcccgcc aggaacaaca
    50521 tcgccagcgt agtcacaaac aacaccacca ccgtaccaag gtctggctgt gccagcagta
    50581 acactgccaa caccagaatc acgcccatcg gtttcaggaa gccgcgcagg ttattacgta
    50641 cttcgtcgcc tttacgcacc agatagttgg cgatataaca aaacagcgac agttttgtca
    50701 gctccgcagg ctggatacgc agcaaaccga gatcgatcca acgcgatgcc cctttaaccg
    50761 agctacccac taccagcacg atcatcagca ggatgataga tccgagcagc atcgtggcac
    50821 tgtagcgttg ccagaactcc atcggcagac gcagcgtaat gatcgccaga atgaatgcca
    50881 ggatcagata gacgccatca cgcttcgcga agaagaacgg atcgttggtt aagcgttgcc
    50941 ctatgggcat tgacgccgag gtcaccatga taaagccaat cgccgcaagg ccgaaggtca
    51001 gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt atctttttcc cgcgagccca
    51061 tcacccagcc ttttagcgcc gtggagatcc agaccaggat actgaatcct ggcaggcgcg
    51121 gcattttcag gcgagggaga gataaacgca tcaacctaac tccttcgcca gacgggcaaa
    51181 ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg ctggcacagg ccggggagag
    51241 cagaaccata tcgcccagct gaacacgcgg agccagcaag cgcatcgctt gttccatggt
    51301 ttcggtttgt tctgccactt ccgggcgtag cgccgccagc tgcgcaccgt cacgaccaaa
    51361 acagtacaaa cgcacgttgt cgccattcag atagcgcgcc agtggactga agtccgccga
    51421 tttaccatcg ccgcccagca acaaatgcag cgtgccgtct acctgcaagc cattcaacgc
    51481 cgcttccgta ctgccgacgt tagtggcttt cgaatcgtta atccagcgta cgccattgtg
    51541 ctccagcacc acttcaaagc gatgcggcag accggtgaag gtggttaacg ctttcaggct
    51601 gctggcgcgc ggcaaaccag ctgcgtctgc cagtgccagt gctgccagcg cattggtgta
    51661 gttatgctgc ccggaaagtt tcatctcttt cacattcagc actttctcgc ctttaacccg
    51721 cagccaggtt tcgccctgct gatgattcag gtgatagtca cccatgttga cgccaaagct
    51781 gacgcagcgt tcatcagcac cgcgaatcgg cattgttaag gcatcatcag cattgacgac
    51841 acaaactttc gcattttcgt aaatacgcaa ttttgccgca cggtattgct gcaaaccaaa
    51901 cggatagcgg tccatatgat cttcagtcac gttcagaatg gtcgccgcca ccgcctgtaa
    51961 gctggaagtg gtttccagct ggaagctcga cagttccagc acgtacagtt cgcactcagc
    52021 atcaagcagc atcaacgctg gcaggccaat attgccaccc acaccaacgt taacccccgc
    52081 cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt ttgccgttag aaccggtaat
    52141 cgccacaatc ggtgcttgtg cttcgcgaca gaacagctcg atatcgccaa cgatttcgat
    52201 tccggcatca gcggcagcgc ttaaggatgg atgcgcctgt gcaataccgg gactggcgac
    52261 aatcagatct gccgccatca gccattcatc attcagactg cccgtgtggc gttctacggc
    52321 ttcgggtaat ttatccaggc caggcggtgt catacgcgta tccataacgc gcggcgtcac
    52381 accgcgagcg aggaaaaagt ccacgcagga aagcccggtg aggcccaggc cgataatgac
    52441 gacattttta ccctgataat cagccatgat taacgtacct tcagcgttgc cagaccaatc
    52501 agaaccagca tcagcgaaat aatccagaaa cgcacaatga cgcgcggttc cggccagcct
    52561 ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa tacgttgtcc gcgcagttta
    52621 aaggagccga cctgcaggat gacagaaagc gtttctacca cgaacacgcc ccccataatc
    52681 accagcagga attcctgacg tagcagtacg gcgataatgc ctaacgcacc acctaacgcc
    52741 agcgaaccta catcgcccat aaagacctgc gccggatagg tgttaaacca caggaagccc
    52801 agtcctgccc cgactatcgc cgtgcagaca atgaccagtt cccctgcgtg tcgcagatac
    52861 ggtatatgca agtagctggc aaagttcata ttgccggtcg cccacgccac cagcgcaaaa
    52921 ccaccggcga caaatacggt cggcataatt gccaggccgt cgagaccatc ggtcaggttt
    52981 accgcgttgc cagtacccac aatgacgaag taagccagca gaatgtagaa cagccccagc
    53041 tgcggcatca catctttaaa gaatgggacc accagctgcg ttgcgggcgt gtctttgccg
    53101 gcaaggtaca gggcgaaggc gacacccagc gcaatgaccg acatccagaa atacttccaa
    53161 cgagcgatca accctttggt gtctttacgc accactttgc gataatcatc aacaaagcca
    53221 ataacaccgt aacctaccag caccaccaac acgcaccaga cgtacggatt ggacgggtaa
    53281 gcccacagca gtacggagat cacaatcgcc gtcaggatca taatcccgcc catggtcggc
    53341 gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac gcaccacctg accaaaggaa
    53401 agtttttgca aatgagctat catacgcggg cccatccaca atgagatgaa cagcgcggtc
    53461 agcaggctga cgatggcgcg aaacgtcaga taggaaaaga cgttaaagcc ggaataatat
    53521 ttgaccaaat gttcggccag ccaaactaac atgtcccatt ctcctgtaaa gcgcgtacta
    53581 cctcttccat ggcggcacta cgtgaaccct taactaaaat cgtaattacc tgttgctcag
    53641 caatcagtga tttaagacgc gtaattaacg cagttttatc agcaaaatgt tcgccaacgc
    53701 cgctggcggt gctgatagca tggctttgtt tacccacgct taacacgcgg tcaataccag
    53761 cagcttttgc cgcctcgccc acctgtacat ggcaggcttc gctttcagcg cccagttccg
    53821 ccatatcgcc caccaccagc acgcggtagc ccggcatttc agccagtacc tgaacagcgg
    53881 cggtcattga accgacattg gcgttgtagg agtcgtcgag cagcaactgg ttttctgcca
    53941 gttggatggg gaacagacgg cctggaacag ctttcagatt tgccagcccc gctttgatag
    54001 catcaagcgt tgcgcccacg gacatggaga gcgcagcggc tgccagcgca ttcgcaatat
    54061 tgtgacgccc cggcaacggc agcagaacat cgacgctacc ggttggggtt tgtagggtaa
    54121 attccgtacc gtgcgaggtc acatggatat tggtggcggt gaaatcgctg ttggcggcat
    54181 tgggtgagaa acgccacact ttgcgtgagc caattacgct ctgccagttc agccagtcgt
    54241 tgttgtcggc gttcataatg gcgataccgt tttccggcag gccgctaaag atttcacctt
    54301 tcgctttcgc gacacccgca agcgagccaa aaccttccag atgcgccgct gccaggttgt
    54361 tgaccagcgc agcttccggg cgagtcagac tcacagtcca ggctatttcg ccctgatggt
    54421 tcgcgccaag ttcaataact gcgtaatcgt attccggcgt caagcgcaac aacgtcatcg
    54481 gtacaccgat gtcgttgttg agattgcctg ccgtataaag cgtgttgccg cactggctta
    54541 aaatcgccgc cgtcatctct ttaacggagg ttttgccgga ggaacctgtc agagcaacca
    54601 cgcgcgccgg aacttgctgg cgaacccatg cagccagttc accaaacgcc agacgcgtat
    54661 ccttgacgat taactgcggc aggtcgatat ccagcggacg gctgaccagt agtgcgcccg
    54721 cgccgccagc tttcgcctgg tcggcaaaat catgagcatc gaaacgttcg cctttcaggg
    54781 caacaaacag gcagcccggc gtcagttttc gggtatcagt ggttacagca tcaagggtga
    54841 tatctgcacc ttgcagttca ccgttgagaa tgtcggtaag ttggctaagg gttacgctaa
    54901 tcatgcaatc acccccagca gacgcgccac cgtgacgcga tcggagtagt ccagacgctg
    54961 attgccaaca atctggtaat cttcatggcc tttgcccgcg accagtacca catcattctc
    55021 tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg ccttccatca ctttggcatg
    55081 tccggcatct aacattcccg ccagaatatc gttgatgatg gcacgcggtt cttcggtacg
    55141 cgggttatcg tccgtcacca ccgccacgtc agcaaactct tcggcaattg cgcccatcag
    55201 tggacgctta cctttatcgc gatcgccacc acagccaaag acgcaccaca gcttgcccgc
    55261 acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca tccggcgtat gcgcgtaatc
    55321 caccaccacc gtcggtttgc ctggcgcagt gaacacttcc atacgtccgc aaaccggttg
    55381 cagacgcgcg gcggttttca gcagatcagc cagtggatag ccgagtgcca gcagtgtcgc
    55441 cagcgcgagc agcaggttgc tgacgttaaa agcgcccatc agatggcttt caatttcgcc
    55501 atcgccccaa cttgagctaa agcgaatcgt cgcaccgctg tcgtgatagt tcacgtcgat
    55561 cgcttttaac cagcgtccgt gacagttcgg attaatatga tcttccattg ataccgcaac
    55621 cgcgtccggc agttttgcca gccagcggcg gcccacttca tcgtcggcgt taataatcgc
    55681 ctgaccgcaa tgatgttcag aataaagcag ccatttcgca gcttcgtagt gttccatatc
    55741 accatgataa tcaaggtgat cgcggcttaa gttggtaaag accgacgccg caaatttcaa
    55801 tgccgccaca cggtgctgta ccagcccgtg ggaggaaact tccattgcgc aaaacgtcgc
    55861 gccctgctcc accagccccg ccagctcatg ctgaacatcg actgcggaac cggtagtatt
    55921 ttctgtcggg atcactttcc caagcagacc gttaccaacg gtgcccatta ccgcgctggt
    55981 ttcgccaagc agttggctcc actgcgccag cagctgggta gtcgtggttt tgccgttggt
    56041 gcccgttacg cctacgagac gtaaattgtc agagggttca tggtaaaagc ggcccgccag
    56101 tgcagataaa cgctcattga gctggctgag atagatgacc ggtacgccgt gcatttcacg
    56161 gatttcaccg tcggtcgcct catctttcgc tttcgcctct gcaataatgg cagccacacc
    56221 ttgcgctatc gcctgcggga tatatcgacg cccgtccgcc tgatgaccta ctacagctac
    56281 aaagagatcg cccgccgcag ccacacggct gtcgagtgtc atctctcgca gtgctcgcga
    56341 aggtgcgtct ggcacccacg gagcaagaag gtcgcgcaaa ttacgatctg ccacctgtcc
    56401 cctcgccttg attaatcaca aattcatttt tatcgcccgt tgtcagcgca tccggctcga
    56461 tgttcatggt acgcaatacg ccgcccatga tggcaccaaa gaccggcgcg gaaacggcgc
    56521 cgccgtagta tttacccgcc tgcggatcgt tgataacaac aaccagcgcg aagcgcggct
    56581 gactcgcagg cgcaacgcct gcggtataag caatatattt attgatgtag cgaccgtccg
    56641 gcccgacctt tttcgcggta ccggttttaa tggcgatacg atagccttta atcgccgcct
    56701 tcacgccgcc gccgcctggt agcgccacgc tttccatcat atgcaccaca gtgcggacaa
    56761 tggatcccgg gaagacacgt tcaccgggaa ccggggggtc aactttggta atcgacagtg
    56821 ggcgataaat gccgtagctg ccgatagttg cgtagactcg cgctaactgt aacggtgtta
    56881 ccattagccc gtagccgaaa gagaaggtgg ccctctctat gtcagaccac cgttgttttt
    56941 gaggatataa gccactgcgt tctccgacca accccaaatt ggtcgctttt cccagtccaa
    57001 aacgtgagta agtatctact aacgctgagg acggcatcgc taacgccagc ttggaaacac
    57061 cgacgttact cgacttctgt aataccccgg tcagggttaa ttcgctgtag cgtgccacgt
    57121 ctttgatttc gtggccgtta attcgataag gaatggtatt gagtaccgag ttttcccgca
    57181 ccacgccacg ttgcaacgcg gtcattacca ccatcggttt aaccgttgag cccggttcaa
    57241 acacgtcggt gatggtacgg ttacgcatcg cctctttcgg cgtgccgctc agattgttag
    57301 ggttgtatga cgggctgtta gccatcgcca gcacttcacc ggtgttgaca tccaccagca
    57361 cggcgctacc agattcagcc ttgttaaagg ccaccgcgtt gttcagttcg cgataaacca
    57421 gcgcctgcag gcgttcatca atactcagcg ccaggttgtg cgctgcctgg ctgtcagtag
    57481 aagaaatatc ttcaattacg cgaccatagc ggtctttacg cacaatgcgc tcacccggct
    57541 gcccggtaag ccatttatcg aaactcttct caacgccctc aatcccttga ctatcgacgt
    57601 tagtaaagcc gatgaggtga gcagtcactt cgccggacgg atagtaacgg cgagactctt
    57661 cacgcagatg aatccccggc agtttcagtt ttttgatgta gtccgccatg tcagggttca
    57721 cctgacgcgc cagataaata aaacgccctt tcgggttggc gttaatgcgg gctgaaagct
    57781 gatccagcgg aatattgagc gcgttagcca gcgccttcca gcggtcaccg acgctgatac
    57841 cgccagcgtc atgcacttct ttcgggtcag cccaaatcgc ttttaccggc acgctcaccg
    57901 ctaacgggcg accagaacgg tcagtaatca tgccgcggga ggtggaaact tgctgaacgc
    57961 gaagagaacg catgtcgccc tctttcacca gcatatccgg ggagataact tgtaaccacg
    58021 ctacgcgtcc gagcagaaaa gccagcgcca ggagaataca gccgcataac aacgcaaaac
    58081 gccaactgat aaagttggca tgttcttcct gacgttttgg tttctgcgtt ttcgccgctg
    58141 ctttcatgcg tcgcgtttat ccttattttt gcactacgat attttcttgt gacggatcaa
    58201 catgctgcat ttgcagcttt tccgtggcga tcctttccac ccggctatgg tcgccgagcg
    58261 cattctcttc aaggatcagg ttgcgccatt caatgtctaa agcatctcgc tccagcacca
    58321 gttgttcgcg ctgagcggtc agtaaacggg tatggtgcgc cgtggttacc acagtcaccg
    58381 ccgtcaaaat aatgcaaatg aacaggcaga gtggcagctt cccaaatcgc aaaagatcgt
    58441 caccgataac accaggcaat gcatggcgct cgtggcttcc catcgatcct ttaactttgc
    58501 ttagagcttc tgtcactctg ctgatcatgc attcgtcctc tctgcaatac gcagaactga
    58561 actacgggca cgagggttct cagccacctc ttcttcgccc ggcattaact tgcctagtgc
    58621 tcgcagctga cggccaccca gttttttgag ctgctcttca gtcatcggta accctgccgg
    58681 aacttgcgga ccgcggctgt tttcacgcat aaaacgtttc acaatacggt cttccagcga
    58741 gtggaagctg atgatcgaaa gccgcccacc cggggccagc acgttgagcg agctttttag
    58801 cgcctgctct atctcctcca gttcactgtt tacccaaatg cgcaccgcct ggaaggtacg
    58861 ggtcgcggga tgtttaaact tatctttcac cggcgttgca acagccacga cttccgccag
    58921 ttctttggtg cgggtcatcg gctgttcgcg gttacgctcg acaatggcgc gggcaatgcg
    58981 tttggcaaaa cgctcttcac cataggtttt caatacccaa gcgatatcgg cttcttctgc
    59041 ggtttgtagc cattcagcgg ctgactgccc acgggttggg tccatacgca tgtccagcgg
    59101 accatcgcgc ataaaggaaa agccgcgctc agcatcatca agttgcggtg aagagacgcc
    59161 aagatcgagg agaatgccgt cgatcttgcc gataagatcg cgctcggcaa cgtattcgcc
    59221 cagcgcggag aaaggtccgt ggatgatgga gaagcgcgga tcatcaatag tcttcgcaac
    59281 ggcgatagcc tgcgggtcgc gatcgatcgc cagcaaacgc ccctcttcgc caagctgcga
    59341 gaggatcaga cgtgagtgac caccgcgacc aaaagtccca tcaatgtaga tgccatcagg
    59401 acggatattg aggccattaa cggcttcatc cagcagcacc gtagtatgtt tatagttttc
    59461 catcatttta tagagacaag tcctgcagtc gctccgataa gtctccggta gccaactgct
    59521 ctgcgtcgat atcttccttg acctgttgat gccaggttgt ttcatcccac agctcaaact
    59581 tgttgaactg tccaaccagc atcacttctt ttgtcagccc ggcatgttgc cgcagtactg
    59641 gcgcgattaa caatcgacct gcgccatcca tctgacattc gctggcatga cctaacagta
    59701 ggcgctgcac acggcgctca accgggttca tgctcgacag acgcgataat ttttgctcga
    59761 taatttccca ttcaggcagg gggtaaagca gcaggcacgg gtgatgaatg tcaatggtgc
    59821 aaaccatttg accgacagcg ttctcaagca gctgttcccg gtaacgggta ggcactgata
    59881 agcgcccttt gctgtcgaga ttgactaacg ttgctccccg gaacatgcca gcctcacccc
    59941 ttattcccac tttgccccac aatttcccac tgaaaggagt ttacggagcg gaggaaaagc
    60001 ttgtcaagcc agtcacaagg cataccgagg taaaaaaccc gatgtttacg gctaatatca
    60061 gcgttgttta ttttacgtcc aacgtgcgca gcaaattaac gttatgaata gctgtaagaa
    60121 aaaatccctc atcgtcatca ccgttttaaa atgactcact attttccaca gcaaatataa
    60181 agtgtcagtt tgcgacgcga gcggcatttt aggacatatc ttccccggtt aacagtccct
    60241 gttgcgtctg tgtggcgctt gatgccagcg ggtttgcgca aaagcaaaga aaagtgcttg
    60301 ttaaatcggc aattcatctc tgcatgtaac aaaataatgc aaaaacgcgc tgtcgatatc
    60361 acttaattat tgtgaatttc agctggtgag ttaattaacg ggcgggttta aaagaactta
    60421 tcaataatag tcaggaatag tcttatttac tttaaggata ttgatgtcca ttcccgtact
    60481 ctacgc
//
LOCUS       DAJVRF010000006        59208 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJVRF010000006 DAJVRF010000000
VERSION     DAJVRF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59208
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.006"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..252)
                     /locus_tag="OQN13_000333"
     CDS             complement(<1..252)
                     /locus_tag="OQN13_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1613800.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSA"
     gene            359..655
                     /locus_tag="OQN13_000334"
     CDS             359..655
                     /locus_tag="OQN13_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1613801.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(789..1214)
                     /gene="arsC"
                     /locus_tag="OQN13_000335"
     CDS             complement(789..1214)
                     /gene="arsC"
                     /locus_tag="OQN13_000335"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCS1613802.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1227..2516)
                     /gene="arsB"
                     /locus_tag="OQN13_000336"
     CDS             complement(1227..2516)
                     /gene="arsB"
                     /locus_tag="OQN13_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCS1613803.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2570..2923)
                     /gene="arsR"
                     /locus_tag="OQN13_000337"
     CDS             complement(2570..2923)
                     /gene="arsR"
                     /locus_tag="OQN13_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCS1613804.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            complement(3462..3635)
                     /locus_tag="OQN13_000338"
     CDS             complement(3462..3635)
                     /locus_tag="OQN13_000338"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1613805.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            3663..3746
                     /locus_tag="OQN13_000339"
     CDS             3663..3746
                     /locus_tag="OQN13_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCS1613806.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3800..5152)
                     /gene="gorA"
                     /locus_tag="OQN13_000340"
     CDS             complement(3800..5152)
                     /gene="gorA"
                     /locus_tag="OQN13_000340"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCS1613807.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5224..6066)
                     /gene="rlmJ"
                     /locus_tag="OQN13_000341"
     CDS             complement(5224..6066)
                     /gene="rlmJ"
                     /locus_tag="OQN13_000341"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCS1613808.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6269..8311
                     /gene="prlC"
                     /locus_tag="OQN13_000342"
     CDS             6269..8311
                     /gene="prlC"
                     /locus_tag="OQN13_000342"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCS1613809.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKSVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8319..9071
                     /gene="rsmJ"
                     /locus_tag="OQN13_000343"
     CDS             8319..9071
                     /gene="rsmJ"
                     /locus_tag="OQN13_000343"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCS1613810.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9120..10589)
                     /gene="dtpB"
                     /locus_tag="OQN13_000344"
     CDS             complement(9120..10589)
                     /gene="dtpB"
                     /locus_tag="OQN13_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCS1613811.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10907..11341)
                     /gene="uspA"
                     /locus_tag="OQN13_000345"
     CDS             complement(10907..11341)
                     /gene="uspA"
                     /locus_tag="OQN13_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCS1613812.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11732..12067
                     /gene="uspB"
                     /locus_tag="OQN13_000346"
     CDS             11732..12067
                     /gene="uspB"
                     /locus_tag="OQN13_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCS1613813.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12311..13810)
                     /gene="pitA"
                     /locus_tag="OQN13_000347"
     CDS             complement(12311..13810)
                     /gene="pitA"
                     /locus_tag="OQN13_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCS1613814.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14042..15244
                     /locus_tag="OQN13_000348"
     CDS             14042..15244
                     /locus_tag="OQN13_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCS1613815.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15560..16543)
                     /locus_tag="OQN13_000349"
                     /pseudo
     CDS             complement(15560..16543)
                     /locus_tag="OQN13_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            16943..18550
                     /locus_tag="OQN13_000350"
     CDS             16943..18550
                     /locus_tag="OQN13_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS1613816.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVN
                     MLNDFSQIDNDLGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKSDITYKVNGLS
                     IIPENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNAN
                     TAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNKLLYN
                     LLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMDG
                     NKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCHYDSETKVL
                     TSHHGIIRNEENECYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRS
                     NAVASTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTM
                     GLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            18812..20434
                     /locus_tag="OQN13_000351"
     CDS             18812..20434
                     /locus_tag="OQN13_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS1613817.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            20800..21867
                     /locus_tag="OQN13_000352"
     CDS             20800..21867
                     /locus_tag="OQN13_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1613818.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELSWPDDLVVRLPQ"
     gene            21864..24599
                     /gene="rbbA"
                     /locus_tag="OQN13_000353"
     CDS             21864..24599
                     /gene="rbbA"
                     /locus_tag="OQN13_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCS1613819.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            24599..25723
                     /locus_tag="OQN13_000354"
     CDS             24599..25723
                     /locus_tag="OQN13_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1613820.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            25796..26069
                     /locus_tag="OQN13_000355"
                     /pseudo
     CDS             25796..26069
                     /locus_tag="OQN13_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            26066..26425
                     /locus_tag="OQN13_000356"
     CDS             26066..26425
                     /locus_tag="OQN13_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCS1613821.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(26545..26946)
                     /gene="nikR"
                     /locus_tag="OQN13_000357"
     CDS             complement(26545..26946)
                     /gene="nikR"
                     /locus_tag="OQN13_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCS1613822.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(26952..27758)
                     /gene="nikE"
                     /locus_tag="OQN13_000358"
     CDS             complement(26952..27758)
                     /gene="nikE"
                     /locus_tag="OQN13_000358"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCS1613823.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(27755..28519)
                     /gene="nikD"
                     /locus_tag="OQN13_000359"
     CDS             complement(27755..28519)
                     /gene="nikD"
                     /locus_tag="OQN13_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCS1613824.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(28519..29352)
                     /gene="nikC"
                     /locus_tag="OQN13_000360"
     CDS             complement(28519..29352)
                     /gene="nikC"
                     /locus_tag="OQN13_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCS1613825.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(29349..30293)
                     /gene="nikB"
                     /locus_tag="OQN13_000361"
     CDS             complement(29349..30293)
                     /gene="nikB"
                     /locus_tag="OQN13_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCS1613826.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(30293..31867)
                     /gene="nikA"
                     /locus_tag="OQN13_000362"
     CDS             complement(30293..31867)
                     /gene="nikA"
                     /locus_tag="OQN13_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCS1613827.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTPQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(31978..32565)
                     /gene="acpT"
                     /locus_tag="OQN13_000363"
     CDS             complement(31978..32565)
                     /gene="acpT"
                     /locus_tag="OQN13_000363"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCS1613828.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(32620..33669)
                     /locus_tag="OQN13_000364"
     CDS             complement(32620..33669)
                     /locus_tag="OQN13_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCS1613829.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            33801..35018
                     /locus_tag="OQN13_000365"
     CDS             33801..35018
                     /locus_tag="OQN13_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1613830.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(35022..35579)
                     /gene="dcrB"
                     /locus_tag="OQN13_000366"
     CDS             complement(35022..35579)
                     /gene="dcrB"
                     /locus_tag="OQN13_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCS1613831.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(35652..36317)
                     /locus_tag="OQN13_000367"
     CDS             complement(35652..36317)
                     /locus_tag="OQN13_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCS1613832.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            36538..36783
                     /gene="tusA"
                     /locus_tag="OQN13_000368"
     CDS             36538..36783
                     /gene="tusA"
                     /locus_tag="OQN13_000368"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCS1613833.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(36885..39083)
                     /gene="zntA"
                     /locus_tag="OQN13_000369"
     CDS             complement(36885..39083)
                     /gene="zntA"
                     /locus_tag="OQN13_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCS1613834.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(39157..39783)
                     /locus_tag="OQN13_000370"
     CDS             complement(39157..39783)
                     /locus_tag="OQN13_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCS1613835.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            39924..40283
                     /locus_tag="OQN13_000371"
     CDS             39924..40283
                     /locus_tag="OQN13_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCS1613836.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(40286..40555)
                     /locus_tag="OQN13_000372"
     CDS             complement(40286..40555)
                     /locus_tag="OQN13_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCS1613837.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(40545..41141)
                     /gene="rsmD"
                     /locus_tag="OQN13_000373"
     CDS             complement(40545..41141)
                     /gene="rsmD"
                     /locus_tag="OQN13_000373"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCS1613838.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            41291..42787
                     /gene="ftsY"
                     /locus_tag="OQN13_000374"
     CDS             41291..42787
                     /gene="ftsY"
                     /locus_tag="OQN13_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCS1613839.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            42790..43458
                     /gene="ftsE"
                     /locus_tag="OQN13_000375"
     CDS             42790..43458
                     /gene="ftsE"
                     /locus_tag="OQN13_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCS1613840.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            43451..44509
                     /gene="ftsX"
                     /locus_tag="OQN13_000376"
     CDS             43451..44509
                     /gene="ftsX"
                     /locus_tag="OQN13_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCS1613841.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINDLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            44754..45608
                     /gene="rpoH"
                     /locus_tag="OQN13_000377"
     CDS             44754..45608
                     /gene="rpoH"
                     /locus_tag="OQN13_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCS1613842.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            45879..46982
                     /gene="livJ"
                     /locus_tag="OQN13_000378"
     CDS             45879..46982
                     /gene="livJ"
                     /locus_tag="OQN13_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCS1613843.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(47181..47564)
                     /gene="panM"
                     /locus_tag="OQN13_000379"
     CDS             complement(47181..47564)
                     /gene="panM"
                     /locus_tag="OQN13_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCS1613844.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            47988..49097
                     /gene="livK"
                     /locus_tag="OQN13_000380"
     CDS             47988..49097
                     /gene="livK"
                     /locus_tag="OQN13_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCS1613845.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            49145..50071
                     /gene="livH"
                     /locus_tag="OQN13_000381"
     CDS             49145..50071
                     /gene="livH"
                     /locus_tag="OQN13_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCS1613846.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            50068..51345
                     /gene="livM"
                     /locus_tag="OQN13_000382"
     CDS             50068..51345
                     /gene="livM"
                     /locus_tag="OQN13_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCS1613847.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            51342..52109
                     /gene="livG"
                     /locus_tag="OQN13_000383"
     CDS             51342..52109
                     /gene="livG"
                     /locus_tag="OQN13_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCS1613848.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            52111..52824
                     /gene="livF"
                     /locus_tag="OQN13_000384"
     CDS             52111..52824
                     /gene="livF"
                     /locus_tag="OQN13_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCS1613849.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            53108..54172
                     /gene="ugpB"
                     /locus_tag="OQN13_000385"
                     /pseudo
     CDS             53108..54172
                     /gene="ugpB"
                     /locus_tag="OQN13_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            54270..55157
                     /gene="ugpA"
                     /locus_tag="OQN13_000386"
     CDS             54270..55157
                     /gene="ugpA"
                     /locus_tag="OQN13_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCS1613850.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            55154..55999
                     /gene="ugpE"
                     /locus_tag="OQN13_000387"
     CDS             55154..55999
                     /gene="ugpE"
                     /locus_tag="OQN13_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCS1613851.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            56001..57071
                     /gene="ugpC"
                     /locus_tag="OQN13_000388"
     CDS             56001..57071
                     /gene="ugpC"
                     /locus_tag="OQN13_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HCS1613852.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            57068..57811
                     /gene="ugpQ"
                     /locus_tag="OQN13_000389"
     CDS             57068..57811
                     /gene="ugpQ"
                     /locus_tag="OQN13_000389"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCS1613853.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(57798..58238)
                     /locus_tag="OQN13_000390"
     CDS             complement(57798..58238)
                     /locus_tag="OQN13_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCS1613854.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            58358..>59208
                     /locus_tag="OQN13_000391"
     CDS             58358..>59208
                     /locus_tag="OQN13_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCS1613855.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRG"
BASE COUNT        14433 a        14947 c        15974 g        13854 t
ORIGIN      
        1 cgctgacaac aatccacgta acatgggtat tgtcgagcaa ctacagaacg gggtaacggc
       61 tcccaacaga gcagccagga gatagcctct cccttttctg gcccccatca tctgctggat
      121 tttatggtct ggcacctttt gtcttatcag gctgacaccg gcactaatca ctataaacag
      181 caacgaaagc tcgacggcga gaaacacaaa catctctgcg gcatcttgca gcatcgcaag
      241 ccaactactc atacgtaacc tcattggata aatctatatt tccagaataa tcgaaatgaa
      301 attcagatca agatatttct ggtattatcg aaatatcaac cagacagagg ggtgtcatat
      361 gcagttggaa gaagtcgcta aagcgctgaa agagctaggt cacccgaccc gtttgttcat
      421 atttaaacat cttgtcaaag ccggggaaca agggttgccg gtcggtgagt tgcaaaagca
      481 gcttgggata cctggctcca cactcagcca ccatatatca gcactggtat cggtaggact
      541 ggtcaaacaa aaccgtgaga gtcggacgct gatgtgtgtt tcgcaatacg caatgctgga
      601 agcgataatt gaattcttac gcgaggagtg ctgcgtaaat agcaaaattg cctgatgcgc
      661 tacgctctac atatttcatg ccatatattg aatttgcacg gttttgtagg tcggataaga
      721 cgcgttagcg tcgcatccga cataaacaaa gcgcacttta ccaactatcg gcagtggata
      781 tcgccgcttt atttcaggcg cttacccgct tcatcaacca ctttctcgcc atcttccttg
      841 gagaatgcgc ctttttgcgc atctggcaga atttccagca ccacttctga agggcggcac
      901 aggcgagttc ccagcggcgt caccacaatc gggcgattaa tcagaatcgg gtgctgaagc
      961 ataaagtcga ttaaccgatc gtcagtaaat ttatcttccg caaggcccag ctcctcatac
     1021 ggttcgacgt ttttacgcag cagcgcgcgt acggaaatcc ccatatcggc aatgagtttg
     1081 accagttcat cgcgcgttgg cggagtttcc agataatgga taatggtcgg ttctgtgccg
     1141 ctgttgcgga tcatctccag cgtattacgc gacgtgccgc aggccgggtt gtgataaatg
     1201 gtaatgttgc tcatatcagt atctcattac aaagtgaaag agagacgtag cgccagcgca
     1261 gccagcgtca caaacagcac aggcagggtc atgataatcc ctgtacggaa gtaatatccc
     1321 cagctgatag tcatattctt ctgcgaaagt acgtgcagcc agagtagcgt agccaggcta
     1381 ccaattgggg taattttcgg tcccaaatcg cagccaatca cattggcata aaccatcgct
     1441 tctttgataa cgccagatgc cgtgctgcca tcaatggaca acgcgccaac cagtaccgtc
     1501 ggcatattgt tcataataga agagaggaag gcggtgagga atccggtgcc gagcgtcgcg
     1561 gcccacaggc cgttatccgc cagcacgttg agtacgccag aaagatattc cgttaatccg
     1621 gcattgcgca ggccataaac caccagatac atgccgagcg agaagatgac aatctgccag
     1681 ggggcaccgc gcaggacttt acccgtatta atcgcatgac cgcgtttagc gacgacaaat
     1741 aatatcagcg cgcccacagc tgcaatggcg ctcaccggaa tgccgagcgg ttccaggacg
     1801 aaaaatccca ccagcagaag cagtaaaaca acccagccag ttttgaacgt agcaggatct
     1861 ttgatcgctt ctgcgggaga tttcagcagc gccatatcgt agttctgcgg aatatctttg
     1921 cgaaaataga gatgtagcat caccagcgtg gcaacaatcg cggcgatatc caccggcacc
     1981 atcaccgagg cgtattcgcg aaagccgagg ccaaagaaat cagcggaaac gatattcacc
     2041 aggttggaga caataagcgg caggctggcg gtatcggcaa tgaatccggc cgccatcacg
     2101 aacgccagcg tcgtgccttt actgaatcct aaagccagca gcatggcgat gacaatcggc
     2161 gtcaaaataa gcgccgcgcc atcattggca aacagggcgg caacggcagc accgagcagg
     2221 acaatccagg taaacagcaa gcgaccacga ccattacccc agcgtgagac gtgcagcgcc
     2281 gcccattcaa aaaagccgga ctcatccagc agcaggctga tgataatgac ggcgataaac
     2341 gcagccgtcg cgttccagac gatattccac accaccggaa tatcacccgg atgaaccacg
     2401 cccgtaacta acgccagtac tgcgccgagc gttgcactcc agccgatgcc taaacctttc
     2461 ggctgccaga taaccaatac gatggtcagg acaaagatag cgcctgccag taacataatg
     2521 cctcccggat aaaacacatc tgaaaattca tatgtgttta gctaaatttt taactgcaaa
     2581 tgttcttact gtccccggaa cagttttgtc gagccaggtt gcggacaatc gcctgaacct
     2641 tttcctgttc acatcgccag gcctcatcaa taattttcgc cgcccatgct ggaatatgcg
     2701 gtgataagcg gtaatgaacc cacttacctt gcttgcggtc cagcaatagc ccgctttcac
     2761 gaagcaatgc caggtggcgg gagatcttgg gctgcgactg atcgagggca gtacaaagat
     2821 cgcagacgca taactctccc agctcgctga gcagtaaaac gatgcccaga cgggtttcat
     2881 cagcaagaat tttgaacaat tggatgggta acagaaatga catattgcgc tcctgattgt
     2941 ggcaggtagt gtctctcttc gaagcggata agtcaaaaac atatatgact taacgaatgt
     3001 gtaagtgcag aggaaggtaa taggtgtgaa ttttgagttg gctattcatt tgaaaggcgg
     3061 tttgaatcag gacgtaaaaa aaccgacttt gcgtcggttt tttactcttc agcctgagtt
     3121 ggtggctctg gctggagtga gaatgcccac atctaacggt tcaatactat tagccagtgg
     3181 ctaacattgc aagaattagc gatttcttca gctggcggag ggggcacttc ggtatatatc
     3241 gcaatgacga tttatatact ttcactgggt catcgtcata ttaagccttg tccgaatccg
     3301 gatgtaaaaa aaccgacttt gcgtcggttt ttttactctt cagccctgag ttggtggctc
     3361 tggctggagt gagatagtca tcatctaacg catcaatgct aataccatac tgaaactatt
     3421 gcaaggacat gctggtttta taacctgaat gtactgtatg attatccagt tagctctgag
     3481 gcattttcac tctggtaatg cgcataaacg ctttcaaagt cctggtcaga agtacgggtg
     3541 gtgccgttaa ctgatgctct ggccggagtg agatagtcgt catctaacaa tgagacatgc
     3601 gccgtgacag gcagtggatg agtaagcgga tgcattctca ctccatcgca tggagaaaac
     3661 gggtgattga taaagcaatc atcgttcttg gagcgttaat tgcgctgctg gaactgatcc
     3721 gctttctgct tcagcttctg aactgatagc ggaaacgtaa ttaagggcta agagcacact
     3781 actcttagcc ctttaacatt tagcgcatgg tcacgaactc ttctgccgcc gttgggtgaa
     3841 tggcgacggt attgtcgaag tcttttttgg ttgcgcccat cttcagcgcc accgcgaagc
     3901 cctgcaacat ttcgtccata ccaaagccaa tgccgtgaat accgacaatc ttctcttctg
     3961 gcccaacgca caccagcttc atgcggcacg gctggcggtg agtggtgacg gcggtataca
     4021 tcgcggtgaa agaggattta tacactttca cctgatcgtc gccgtactgc tcgcgcgcct
     4081 gcggttccgt taaaccaaca gtaccaatcg gcggatggct gaagaccacg gtcggaatgt
     4141 tgctgtaatc cagatgctca tccggcttgt tattaaacag gcgttcagag agacggcgac
     4201 ccgctgcaac tgctaccggt gtcagttcca ctgcacccgt gttatcgccc accgcgtaaa
     4261 taccttcaat attggtgttt tgatatttat cgacgacgat atagcctttt tcgttagttt
     4321 taacgccagc ggcttctagg ttgatgttgt cattggcagg ctcgcgacca atcgcccaaa
     4381 tcaggcaatc caccgtttca ctgcgaccat cttccagctc cagcgtcagg ctaccatcgg
     4441 catttttcac taccgctttc gggatggcgt tggtgtgcag ctgcgggcct tcggcgttca
     4501 tcacttcgac cagcgtttcg gaaatcatcg ggtcgaagct gcgcagcggc gcatgtttac
     4561 gcacaaacag atgcgttttc gcgccgaggc cgttaatcac gcccgccagc tcaacggcga
     4621 tgtaacccgc gccaacaacc gccacgcgct ctggcaaagc aggaagggcg aagaagccat
     4681 cagaatcaat accgtattcc acgcccggaa tatccgggtg gctcggacga ccgcctgtgg
     4741 cgatcagaat atgatcggcc gtgatggttt cgccgtttac ctccagcgtt ttggcatcaa
     4801 cgaagcgggc aaagcctttg attacatcaa cgttattttt accgagcacg ttttcatagg
     4861 aagtatgaat acggtcgata taggcggtac ggctggcgat caacgtttcc cagttgaact
     4921 tgttgatggt ggtatcgaaa ccataatcag ggccgtacat atggatcgct tcacggattt
     4981 gcgccgcgtg ccacatcact tttttcggca cgcagccgac gttcacgcag gtgccgccca
     5041 gctctttggc ttcaatcagt gcgcatttct ggccgtacat agccgcgcgg ttgatggagg
     5101 cgataccgcc gctgccgccg ccgatggcga tgtaatcata gtgtttagtc atgacagagt
     5161 gtccttatcg ttgattaccg cgattgtagc gcgagacgta ataggtgcca gcaatggctg
     5221 caattactcc ggcacgatcc agcttacggt ggcgtgcccg gtgcctgccg gaaccagttt
     5281 gctgtgcagc cacggcagca cgttattcat ctgctgttcc agtttccacg gcgggttaat
     5341 cacaatcatg ccggaagcgg tcatgccacg gcgatcgctg tctggcagta ccgccagttc
     5401 aatttgcaga attttgcgaa taccggtcgc ttccagatcg tggatcatgc gcttaatttg
     5461 ctgacgcagc accaccggat accacagtgc gtaagtacca gtggcgaaac gtttgtaacc
     5521 ttctgctatc ccgctgacca ccgcctgata gtcggttttc atttcatacg gcgggtcgat
     5581 aaggattaaa ccacggcggg aaaccggcgg aagtttggcc ttaagctgct ggaaaccgtc
     5641 ggctttttcg acgcgcgcac ggctgtcttt ctgaaattca gaacgcagca acgggtaatc
     5701 gctcgggtgc agttcggtca gttgcaggct gtcttgttcg cgcagtagct ggcgagcaat
     5761 caacggcgac ccggggtagt aacgcaactg accgctacgg ttgaagtgtt ttaccacatt
     5821 gatgtacgcc tccagttctg cgggcaaatc gtcctgctgc cagatacggg cgataccttc
     5881 gagatattcg ccggtacgct cggcatgttc gctgcctaac tgataacgcc cggcccctgc
     5941 gtgggtatcg agatagagaa acggtttatc tttctctttc agcgactcga tgatcaggct
     6001 ctgaacggta tgtttaagga cgtcggcgtg gttgccagcg tgaaagctgt ggcgataact
     6061 gagcatgggt aaaggtgttc cggtaagtaa aaatcggccc gtattcgggg cgcacaaaag
     6121 cgtatcagga cagtataccg aaaagaggcc gccgccgcga aagcgtaacg tttctcattg
     6181 aaattcacta cacttaaccc catgctacac acattatgta aagcgcctgt tgagcgcttc
     6241 cttaacctct ttaaccagga ctgcgcgaat gacgaatccg ttactgactc cctttgaatt
     6301 gcctccgttt tctaaaattc tcccggaaca tgtcgttcca gccgtgactc aggcgctgaa
     6361 cgattgccgc gaaaatgtgg cgcgcgtagt agcgcaaggg gcaccgtaca cctgggaaaa
     6421 tctctgccag ccgttggcgg aagtggacga tgtgttgggg cgtatcttct ccccggtcag
     6481 ccacctgaac tcggtgaaaa atagcccgga actgcgtgaa gcctacgagc aaaccctgcc
     6541 gctgctgtcg gaatacagca cctgggtagg gcaacatgaa gggctgtata aagcgtaccg
     6601 cgacctgcgc gatggcgatc attacgccac gctgaacacg gcgcagaaga aagcggttga
     6661 taacgccctg cgtgattttg aactctctgg cataggtctg ccgaaagaga aacagcagcg
     6721 ttacggcgaa attgctaccc gtctttctga actgggcaac cagtacagca acaacgtcct
     6781 cgatgcgacg atgggctgga ccaaactcgt taccgacgaa gcggggctgg cggggatgcc
     6841 agaaagcgcg ctggctgcgg caaaagccca ggccgaagcg aaagagctgg aaggctacct
     6901 gctgacgctg gatatcccaa gctacctgcc ggtaatgacc tactgcgaca accaggctct
     6961 gcgcgaagag atgtatcgcg cttacagcac ccgcgcctcc gatcaaggcc cgaacgccgg
     7021 taagtgggat aacagcaagg tgatggaaga gatcctcgcg ctgcgtcacg aactggcgca
     7081 actgctgggc tttgaaaact acgcctttaa atcccttgcc actaaaatgg cagaaaaccc
     7141 gcagcaggtg ctggatttct taaccgatct ggcaaaacgc gcgcgtccgc aaggcgaaaa
     7201 agagctggag caactgcgtg ccttcgctaa agccgaattt ggtgtcgatg agttgcagcc
     7261 gtgggatatc gcttactaca gcgaaaaaca gaaacagcac ctctacagca tcagcgacga
     7321 gcagctgcgt ccgtacttcc cggaaaacaa atcggttaac ggcctgtttg aagtggtgaa
     7381 acgtatttac ggcatcaccg ctaaagagcg taaagaggtt gatgtctggc atccggatgt
     7441 acgtttcttc gaactgtatg acgaaaataa cgaactgcgc ggcagcttct acctcgatct
     7501 gtatgcccgt gaaaacaagc gcggcggggc gtggatggat gactgcgtag gccagatgcg
     7561 taaagctgat ggttctctgc aaaaaccggt cgcgtatttg acttgtaact tcaaccgccc
     7621 ggtaaatggt aaaccggcgc tgttcactca cgacgaagtg atcaccctgt tccacgagtt
     7681 cggtcacggc ctgcaccata tgctgacccg catcgaaacc gctggtgttt ccggtatcag
     7741 cggtgtgccg tgggatgcgg tcgaactgcc gagtcagttt atggaaaact ggtgctggga
     7801 gccggaggcg ctggcgttta tctctggtca ctatgaaacc ggcgaaccgc tgccgaaaga
     7861 gttgctggat aaaatgctgg cggcgaagaa ctaccaggcg gcgctgttta ttctgcgtca
     7921 gctggagttc ggcctgtttg atttccgcct tcatgccgag ttccgcccgg atcagggggc
     7981 aaaaatcctc gaaactctgg cagaaatcaa gaaactggtt gccgtggtgc catctccgtc
     8041 ctggggccgt ttcccgcacg ctttcagcca tattttcgcc ggtggttatg cggcaggtta
     8101 ctacagctac ctgtgggctg acgtactggc ggcagatgct ttctcgcgct ttgaggaaga
     8161 gggcattttc aaccgtgaaa ccgggcagtc gttcctcgac aacattctga gccgtggcgg
     8221 ttcagaagag ccgatggatc tgttcaaacg cttccgtggt cgtgaaccgc agctggatgc
     8281 gatgctggag cattacggca ttaagggctg atcattcagt gaaaatctgc ttaattgatg
     8341 aaacaggcgc cggagacggc gccttatctg ttctggcggc ccgctggggg ctggagcacg
     8401 atgaagacaa cctgatggcg ctggtgttaa cgccggaaca tctggaattg cgcaagcgtg
     8461 atgagccaaa acttggcggc atctttgttg attttgttgg cggagcgatg gcgcaccgac
     8521 gcaaattcgg cggtggtcgc ggtgaggcgg tggcgaaagc ggtgggcatt aaaggcgatt
     8581 atttgccgga tgtggtggat gccactgcag gactggggcg cgatgccttt gtgctggctt
     8641 cagtcggttg ccgcgtgcgg atgctggagc gtaatccagt tgttgccgcg ctgctcgacg
     8701 acggcctggc gcgtggttat gcggatgcgg aaatcggcgg ctggttgcag gagcgtttgc
     8761 agttaattca cgcctccagc ctgacggcgc taaccgatat taccccacgc ccgcaggtgg
     8821 tctatctcga cccgatgttc ccgcataagc agaaaagcgc gctggtgaaa aaagagatgc
     8881 gtgtctttca gtcgctggtg gggccggatc ttgatgccga tggattgctg gagcctgcac
     8941 gcttgctggc gaccaaacgc gtggtagtaa agcgcccgga ctacgcgcca ccgctagcga
     9001 atgtcgccac gccaaacgcg gttgtgacta aagggcatcg ctttgatatc tatgcaggta
     9061 cgcccgtgta aaagtaaagc agggtagcga taacgctacc ctgctttctg caagaataat
     9121 taatggcttt ccggcgtcgc aatcatgcgt ttcagccacg gcaccatcaa cagcatcact
     9181 actgcaacgc ccagcgtaac caggccaatc ttaccaaaca cgttggtata gacgggtaac
     9241 gtctcaagcg gatcggtaat gttgtccggc actgcggtaa atgttgccac atagccgccc
     9301 agcaagaatg cggcagcctg cgtcaggaac cacatcccga gaataaagcc catcaaatgc
     9361 tgcggcacca gggcagcaat catcgccagg ccaagggcgc taataaacaa ttcacccagg
     9421 ctctggaata aatacaccag cacgataaac catggcgatg tcagcccttg tgcatccgca
     9481 aaccacattc ccgcagctgc cgccgtcaaa aagcccagtg agcacataaa catgccgaga
     9541 gtaaatttca tcggcatcga gaggtctttg cctttgttac ccagatgcgt gtaaatgcct
     9601 gccagtattg ggctggcgag taccacccag aacgggttaa gcgcctggaa gctgaccggg
     9661 tttatggaaa agccgagaat ttcatgatgc acgttgttga tggcaaagaa gttcagcgac
     9721 gttggcatct gggcgtagag aatgtaaaac accaccgctt cgagcatcag gacaaaggcg
     9781 acaaacattt tattgcgccc ggttttatcc agcttgaatg cctgacgaaa gaagatgatg
     9841 gtgacgacga tggagagaac aatcagcacc agattggcga cttctacgtt gtgcatcagc
     9901 catgcacata cgaagatcat caccacgctg ccaagtaata cgtacaacag tttgctgaag
     9961 ctcatcggct tgaagtcggg ttcagaacca atgtctttca ccattccacg acaggcgatg
    10021 taaaccagta atgcgataat taaccccgcc ccgcacaggt tgtaggtgac tgaataaccg
    10081 tatctatcag cgatcacagg ggccagcgat aacgctatca acgagccgat attgatcgac
    10141 atatagaaca gggtgaatgc gccatcaagc cgcggatctt tcggcggata gcacttcgaa
    10201 agcaagctgg ctgggttagc tttaaacagg ccgttaccga cagcgatagt ccccagggcg
    10261 atgaaaatca ggtcaggctt aagtagcgac atgccggtca tgaagtagcc aatcgccagc
    10321 acaagtgctc cgagaacaat ggtgcgtttg gtccccagca ggtggtcgcc gacatagccg
    10381 ccaatggaaa tgaggccata gaccagcgca gcaaaagcac caaaagtgac aaaagcctgc
    10441 tcttgcgaga atccaagctg tttaacgaag aaaaccgcca gtacgccctg cacgccgtag
    10501 tagccgaatc gctcccataa ctcgacaaaa aagatcatga aaaatgggcg aggttgctgc
    10561 agcatcccca tgggtgttgt tgtattcata tttattaacc ttccaatacc atcctgaaaa
    10621 gtatgaacgc gtaagggata acgcgcgaaa aagtttacat ctgtcagaga ttcttaatat
    10681 tgatgcggtg aatagattaa tacactatta caacagaaaa taaccagctg atcacactaa
    10741 tgatggaaaa tatgatagtg ttaaatgttg tattggcagg agattttgat tgcgtacgca
    10801 gatcgtaggc cagataaggg gtttacgctg catcaggtaa caaggcgcgc caaaaagcaa
    10861 aacgcccgca tatgcgggcg ttcaggaaca cgtcgtgggg ggatgattat tcttcttcgt
    10921 cgcgcagcgg aacaatcagc atatcaacgt gaacggtgtt gatcagctga cgtgcggaag
    10981 acattagttt gctccagaag tcctggtggt gaccacaaac caccagatcc atatcgtatt
    11041 tcttgattgc atcgaccaga acctggccca ggtcgccgct gccgctcagg gtttcagtga
    11101 ttgggtagcc tgcattagtg gaaagctcgg tcagcgcatg atgtgtctct tcagagatgc
    11161 gtttctgcat atcacccaga ttcacatcaa taagcccggt gtataggtca gagtagttta
    11221 catctacgtg gatcagagaa actttcgcat tgtaggggcg agccatagag actgctttct
    11281 ctaccagaac tttgctttcc ggggagagat cgaccgcgat gagaatgtgt ttataagcca
    11341 tagtgttact ccttccataa agttgtcgat gactggccag ctagcgtttc ttgtgccgtt
    11401 acgtcacccg cgtcctgcga actacgtgcg cggggctaac ccagccgtaa agattcagtg
    11461 ttaagaccat ccttacctta tagcgacccg gatgatccgt caatccgcct tgcttacccg
    11521 ttagataaac aaattttcga catgaatata ttgatagtgg ttaaccttct ggaaaaaaaa
    11581 caacctgatc tcctacacta tctatagagc cgctcggatg ttggtcacca caaaagcggt
    11641 ttctccagga cctggtgtta actgattggc agcgtatcgg gagcggtagc cccggggtgg
    11701 aactccgcgg gcaggtcgcc ggggaggagg tatgataagc accgtcgcat tattttgggc
    11761 tttatgtgtc gtttgcattg ttaacatggc gcgctatttc tcatcactac gcgcgttgtt
    11821 agtggtactg cgtaactgcg atccattgct ctatcaatat gttgatggag ggggcttttt
    11881 tacctcacat ggccaaccca acaaacaggt gcgtctcgtt tggtatatct atgcccaacg
    11941 ttatcgcgat catcacgatg atgaatttat tcgccgctgt gagcgggtgc gtcggcagtt
    12001 tattctgacc agcgcattgt gtggtctggt ggtggtcagc ctgattgcat tgatgatttg
    12061 gcattaagca cttcagcaat aataacgcat aaaaaaagcg ggccagtaaa ctgacccgct
    12121 cgagactgat tacaaacgca aaattgcctg atgcgctacg cttatcaggc ctacataatc
    12181 tctgcaatct attgaatttg catgtttttg taggccggat aaggcgttca cgccgcatcc
    12241 ggcatgaaca aagcgcacta tgtcacaatc tgaagcgggc cagctgaccc gctcgttttg
    12301 gtgcgtacga ttacaggaac tgcaaggaga gccagtacag cccgccggaa agcagtaccg
    12361 cagccggaag ggtaaacacc caggccatca gaatactggt tacggtttta cgctgtaagc
    12421 cgccgccatc taccaccatc gtccccgcga cagaagagga gagtacgtga gtagtggaaa
    12481 ccggcatccc ggtataactc gccaggccga tagacactgc cgccgtcatc tgggcagaca
    12541 tcccctgagc gtaggtcatg cctttcttac cgattttctc accgatagtc gttgccacac
    12601 ggcgccagcc aatcatcgta ccgataccta acgccagcgc gaccgccatg atgatccaca
    12661 ccggtgcata ctcgatggtg ctaagcatgt cggacttcag tttcttcaac aggcgctgat
    12721 cgtcagcact cacgccaggc atcttcacca ctttgtcgat agtgtcagaa acgcacagca
    12781 taatgcggcg catctggcta cgttgatcaa gcgacagctt gtcgtagctt tccacatcgg
    12841 tggtcaacat acctttcagg cggttgagcg cgttaatggt attcgacgga tggcagtgga
    12901 actccgcagg ttgcgttgcg ccagcttccg gagccggtac taactgatca gcaccggtag
    12961 cctgtttgag cagcgcagga tgctgctcaa agtaagcttc gacgttgttg atggcatcac
    13021 gggtacgggt gatttcgtag ccagtggcat tcatgttcac cacgaagcct gctggcgcga
    13081 cgccaatcaa taccaacata accagaccaa tgcctttctg accatcgttc gcgccgtgcg
    13141 aaaacgccac gccgatagcg gaaaggatca gcgcgatacg tgtccagaac ggcggctttt
    13201 tcttgccgtc tttcttttca cgctccgctg gggtcaggtg gatacgggcg cgtttcttgg
    13261 tgccgctcca gtagcgacgc agcaagaaaa tcagaccgcc agcaaacacc aggccgacaa
    13321 taggggaaac gatcagagaa ccgaaaatac ttaatacttt cgggatattg agtgcatcca
    13381 ccactgacgt cccggtcatc aacgcattgg ttaaaccaat cccgatgatc gcgccaatca
    13441 gcgtatgaga gctggatgca ggtaaaccaa agtaccaggt acccaggttc cagataatcg
    13501 ccgccagcaa catagagaac accatggcaa ggccatgaga cgatcccata ttaagcagca
    13561 gatccgtcgg cagcatatgc acaatggcat aggcaacact cagaccaccc agcaaaacac
    13621 ccaaaaagtt gaacaccgcc gccataacca cggcgagctg agaacgcatc gcgcgggtat
    13681 agataacggt tgccacggcg ttggctgtgt catggaaacc attgatggct tcgtagaaca
    13741 gcacaaaagc cagtgcaagc aataataaca gcccggtatg caaatccagg ccagcaaaca
    13801 aatgtagcat aggacgttac gccattttga ggacatgaac gcggcgcatt atcagtgact
    13861 ttcgcggcgc gggcaaagtg aaatatagac tttttttgat ttgcctcctg tatggatttc
    13921 actcaaaaaa taattatctt atataattca ggcaaatact tccttttagt aatattgatg
    13981 ctggtgcgac cactgaggaa tctttacaat tcacgcccgt tttttctaag aggagcgcaa
    14041 cgtggaaagg tttgatgcca ttattatagg cgctggtgcg gcgggtatgt tctgttctgc
    14101 gctggcaggt caggcaggac gccgggttct gctgatcgat aatggtaaaa aaccagggcg
    14161 caaaatcctt atgtctggcg gtgggcgctg caactttacc aacctttatg tcgaaccagg
    14221 cgcttatctg agccagaatc cgcatttttg taagtctgca ctcgcacgtt ttacccagtg
    14281 ggatttcatt gatctggtca ataaacacgg catcgcatgg cacgagaaaa cgttagggca
    14341 actcttctgc gatgactccg cgcagcagat tgtcgacatg ctggtggatg agtgcgagaa
    14401 gggcaatgtg accttcagat tgcgtagcga agtgctgagt gtggcggagg atgaaacagg
    14461 cttcacgctt gatctgaacg gcatgactgt cggttgcgaa aagctggtca tggcgaccgg
    14521 tgggctgtca atgccggggc tgggcgcgtc gccgtttggt tataagattg ccgaacaatt
    14581 tggcctcaac gtgctgccga cccgtgcggg tctggtgcca ttcactctgc ataaaccgtt
    14641 gctcgaagag ttacaggtgc tggcgggcgt ggcggtgcct tccgtgatta ccgctgaaaa
    14701 cggcatcgtt ttccgtgaga acttactctt cacccatcgc ggcttgtctg gaccggcggt
    14761 gttgcagatt tccagctact ggcaaccggg tgaatttgtc agtatcaatc tgctaccgga
    14821 tgtggatctc gaaaccttcc tgaatgagca gcgtaacgca catccgaatc agagcctgaa
    14881 aaacacactg gcggttcatc taccgaagcg gttggttgaa cgcttacagc aactcgggca
    14941 aatcccgaat gtttcgctaa aacagctcaa cgtgcgtgac caacaggcac tgattagcac
    15001 attgaccgac tggcgtgtac aacccaacgg cactgaaggc tatcgcactg ccgaagtgac
    15061 gctcggcggc gtggacacca acgaactctc ttcacggacg atggaagcgc gcaaagtgcc
    15121 tgggctgtac ttcatcggcg aagtgatgga cgttaccggc tggctggggg gctataactt
    15181 ccagtgggcg tggtcgagtg cgtgggcttg tgcgcaggat ttgattgcag cgaagtcgtc
    15241 ctgacctgtt acctaaagat gaaaattatt agagccgcat taaaaatgag ctaattttga
    15301 tagtggctat cttgtgatta ttttctaatg agcccgtgaa ctgaaaccct ccaggcttaa
    15361 tataaggtgg aaggaaaggt gattgaaaac tcacccagtg gccagatctt tataaaatat
    15421 gaacaggctg aaaaagacag taactttgtt gtttttttat ccttaaccac tatgcattaa
    15481 tgctgcgtta tttcatgatg cctaagaaaa accagcgtta cgcaaatggt caacgctggt
    15541 tttatccggt acgttgcaat tattttttag cagaacccgc ttctaatatt gaaacgattg
    15601 agaacaacgt aaagcatacc gctcccaaac cgaccagaac tcgcgaagga ataaaatatc
    15661 ccatgtcggt ctgcgccatt tccgcaagaa atgatgccag gaaaaggcaa aacaggcagg
    15721 tgaagacggg tatcatcggt attctgttgg ctaacgaaca ggtacgtctc cagaccagtg
    15781 ccagtagcca gacttttgag aatatgctgt aacagatcat tccgaggcaa ataagaataa
    15841 tgccgggagc cagtcgggcg cttgcatcgg aactgactaa gacgtatatc ccctgcagta
    15901 ccgtgattga gccgagaaaa ataacccaat aacaccacag ccagtgttct ttagttgaaa
    15961 acgtattacg tgtttgatgg acaattgtgg caacaaggcc aattagacag gcacagattg
    16021 cggttaaccc aagcaatacg tgacccgcga cataatgcgg agttatgtca gcactacgta
    16081 acagcgtaat agaccaaatg aaaccgagca gggtgagtaa aacgggaacg gcaatgaggc
    16141 aattaccgat taatgaagaa taagcctgta ccggcgttcc atcgctcttg ctccccgctg
    16201 catttttggg aatgagcaga aagtgaccgg atgacgctgc caccgtcgat acacaggcgg
    16261 caatcatacc aacgccgaaa ataacatggc cggcgacaaa ctcgtctgcc atcacatcat
    16321 tgcctgctaa cagtgcccaa ccccatatca tggtgataat tgaccccgca tagccaataa
    16381 tggggaacaa tgtattgtaa aacgtattaa cgccgcgcgt gagctgcgaa atgataataa
    16441 atgcggtagt gaataatgcc aggcatatgg ccgccagaga aatcagcaca tgacccgcga
    16501 cgaaatactc gctttgccct gaactttcca gcacaaagcc acccgatata tatgttcact
    16561 ttaaactcct caggtatccc tgctaataat gaaatgtgca ttaatgataa tttcagcctg
    16621 ttaatataac gacgagatat cattaaagac aaatgcatag cataactata gcggggttta
    16681 ctgtgccgag tgccattttt taccggttaa aaggccatga taaataaata tttatcctaa
    16741 gattaattct tattctgttg ttttttttac aattatcttc cagggatgga ttgttttcca
    16801 ttatttagta aaagaccgga tagtctcggt gcttaaaatc atcgaacata taagtattat
    16861 tgctttgtat catcgtaaat atgtctttca gacatgtatt gtttattttg ttactaacaa
    16921 gtaaaaatat tacaggttat ttatgaaagt atttcacaat atatttaagc acttctcttc
    16981 aaaccatcaa gataagcatt cagataaagt taacagtcat caacaccatg gcaaggttga
    17041 taaaaaatat cgatcaaaca cagttgaagt taatatgctg aatgactttt cgcaaataga
    17101 taacgattta ggcctgaata tcatctattt cctgcaacat ggtcactgga gggtaaatga
    17161 acatagtctt cagatggaga aaatatggtt ttacaatagt gagccatcta tagatattca
    17221 agaatataat agtttcgccg ataatactac taacacgttt atctttacaa ttataccaga
    17281 caataaccat gtgataaaat tatcgtctcc cattacagtt aagattgagt gtaaggatgg
    17341 ttattatttt attaatttct ccggagataa atcagacata atttataaat cagacataac
    17401 ttataaagtt aatgggcttt ctatcatacc tgaaaatttc tttactcttc ttagcggtaa
    17461 ttttaaagct gactggcgtt gggatgtctc taaagagacg tttcctaagg ataagtttga
    17521 tagctatgtt aaatctgtgt tttctaaaat agacttttat aaacaatgtg gtgtgattaa
    17581 cccacaaaat gccaacacgg cttattttgg cgatacagat ggaagagtag gcgctgtact
    17641 ttatgcattg ctcgtttcag ggcatatagg catcagggaa aagggttgga gtttattatg
    17701 tgaattatta aaacacgaaa acatggcttc atctgcatac atacataaaa ataataaatt
    17761 gctctacaat ttattaaata cgagagacat gatattgaat gagttgcatc aacatgtttt
    17821 tttaaaggac gatgcgataa ccccttgtat cttccttggg gatcatacag gcgatagatt
    17881 tagtactatt tttggtgata aatatatcct tactttgcta aattctatgc gaaatatgga
    17941 tggtaataaa gatagccgta ttaataaaaa tgtcatcgtt ctggctggaa atcatgaaat
    18001 caattttaat ggtaactata ctgcacgact agaaaattat aaactctctg ccggtgatac
    18061 gtataactta attaaaacgc tcgatgtttg tcattatgac tcagaaacga aagttttaac
    18121 cagtcatcac ggaataataa gaaatgaaga aaatgaatgt tattgtctcg gtgctttgca
    18181 ggttccattt aatcagatga aagacccaat tgatcctgag gaattagcaa atatatttaa
    18241 taaaaaacat aaacaacaca tggatgatcg tcttatccat ctaattagaa gcaatgctgt
    18301 agcatccaca cctgtatatg aaaattattt taataacact actgctttca gaccaaaacc
    18361 tgaagatatc ttcaaatgtg gacagacgct caaaaaaact aaacagaaat atggccatta
    18421 tggattaggg gtagatcagc atcaaaaaat tgataattat accatggggc taaattcctg
    18481 gaagatagca cctaacgaac gtggtgataa aaaaggtgtc ccagggctta gttgttttca
    18541 acctcaataa aacttcatga gtgctgtggt atctcttttt aattttgcca gtatttcata
    18601 ggttttctta atcgattgta gaggcatgtt ctgatgacgc ctctacaatc tttagggact
    18661 ttctctgcta tgagataaga aatttcatat tatgctttaa ggctgaatga tttgtgcaaa
    18721 atataattat tttcccatcg aacatgaggt gtatgtcata agattaaatg ttaactatgt
    18781 attcctcgcg gtaaataata tgaggtaaaa gatgaaaatt ggaactgtgg caggcactaa
    18841 cgacagtaca acgacaatca caacaaatga tatggtgcaa gagcatgtta ccaacttcac
    18901 taaagaactg tttgggtata ttgcaaatgg tataggtgat gatatatcaa gtattgccag
    18961 gacaatgctc ggtgaggttg tggaaaaaat agatgattgg caaatagaac ggttccaaca
    19021 atctattcaa gatgacaaaa tttcatttac catacaaacg aatcattcag aaaaatatag
    19081 tatgctatcg ggcatgcgcg ctcatattct acgtaggaat aactgttatc agtttattgt
    19141 gacaattaat agtaaaaatt acggttgccc tcttgataat accgatatta attggtgcag
    19201 cattgtttat ttacttaata atatgacagt aaatgataat gctaatgatg ttgcagttac
    19261 tgagtcgtac aaacccatct ggaactggaa aatatcgcaa tacaatgtat ttgatattaa
    19321 attcgaaaca atgataaaac cccagttcgc tgacagaacc tatttctcca attgttcacc
    19381 tgttgatccc acaagtacca gaccgaccta ttttggcgat accgatggct cagtcggtgc
    19441 agttttgtac gcgctttttg caacgggaca tttgggaatc atggctgaag gagaaaattt
    19501 ccttagccag ttgcttaata tagaagatga agttctcaac gtattgctaa gggagaactt
    19561 taatgagcaa cttgacacta atgttaatat aataatcagt atcctgaaca gaagagacat
    19621 tgttttagaa agtcttcagc cttatttggt tattaataaa gacgcagtca cgccatgtac
    19681 gtttctgggc gaccagactg gcgatcgatt tagtaatatc tgtggcgata aatttatcat
    19741 cgatttatta aaacgcataa tgagtatcaa tgaaaacgtt catgtattag ctggaaatca
    19801 tgaaacgaat tgcaatggta actatatgca gaacttcacg cgcatgaaac cacttgatga
    19861 ggatacctac tctgggataa aagattatcc tgtatgtttc tatgacccca aatacaaaat
    19921 aatggcaaat catcatggga taacttttga tgatcagcga aaacgttaca ttataggacc
    19981 gataacagtc tctagtgatg aaatgaccaa tgctctcgat ccggtcgaat tagcggcaat
    20041 tatcaataaa aagcaccatg ctgtaattaa tggcaaaaag tttaaaaata gccgggctat
    20101 ctcttgtcga tcctttaatc gttatttttc tgtttcgaca gattacagac ctaaacttga
    20161 agcactatta gcatgctcac aaatgttagg tataaatcaa gttgttgcac ataacggtaa
    20221 tggtggaaga gaatgtatag gtgaaacggg gacagtttta ggtcttaacg cccgtgacag
    20281 taaacatgct ggaagaatgt tcagcatgca taattgccaa attaaccctg gtgcagggcc
    20341 tgagataacc actccctgga agtcttacca gcatgaaaaa aacagaaatg gacttatgcc
    20401 tttgatcaga aggcgcacaa tgttacagct ctgaaccata gacaacatga atattaatgt
    20461 tactatctgg ttgagactgt tgataaaacg taaaaaggaa tgctctgttc ctgcaactga
    20521 acaggcgtga aacgctatcc aacaggatgg ataccgtttt gtcagccagt tttatagatc
    20581 acacttatga gtatgcgtgt ttcatttacg gccaggaatg ctgatgatga caacattact
    20641 acccgttttc actaagcctt ccccgttggc gctcaatgct ctgcgcgctg gtcgtatttg
    20701 tcgtttcctt cttatcccgg atggaagaat ccgctgaacg tcgttcggcg gcagttgtaa
    20761 cacgcgtcgt ttgcttgcag ggattatggc gggtcgatta tggataagag taagcgccat
    20821 ctggcgtggt gggttgtcgg gttactggtg gtggctgccg tcgtggcgtg gtggctgttg
    20881 cgcccggcgg gtgtgccgga aggctttgct gtcagcaatg ggcgcattga agcgacggaa
    20941 gtggatatcg ccagcaaaat tgccgggcgt atcgacacca ttctggtgaa agaaggccag
    21001 tttgttcgcg aaggcgaagt gctggcgaag atggatactc gcgtgttgca ggagcagcga
    21061 ctggaagcca tcgcgcaaat caaagaagca caaagtgccg ttgctgccgc gcaggctttg
    21121 ctggagcaac ggcaaagcga aactcgtgcc gcgcagtcgc tggttaatca acgccagtcc
    21181 gaactggact ccgtggctaa acgtcatacg cgttcccgct cgctcgccca acgaggggct
    21241 atttctgcgc aacagctgga tgacgatcgc gccgccgctg agagcgcccg agctgcgctg
    21301 gaatcggcaa aagctcaggt atcggcttct aaagcggcta tagaagcggc ccgtaccaat
    21361 atcattcagg cccaaactcg cgtcgaagcc gcgcaagcca ctgaacggcg tattgccgca
    21421 gatatcgatg acagtgaact gaaagccccg cgtgacggac gcgtgcagta tcgggttgct
    21481 gagccagggg aagtgctggc ggcaggcggt cgggtgctga atatggtcga tctcagcgac
    21541 gtctatatga ctttcttcct gccaaccgaa caggcgggca cgctgaaact tggcggtgaa
    21601 gcccggctga tcctcgatgc cgcgccagat ctgcgtattc cggcgaccat cagttttgtc
    21661 gccagtgtcg cccagttcac gccaaaaacc gtcgaaacca gcgatgaacg gctgaaactg
    21721 atgttccgcg tcaaagcgcg tatcccaccg gaattactcc agcagcatct ggaatatgtc
    21781 aaaaccggtt taccgggcgt agcgtgggtg cgggtgaatg aagaactttc gtggcctgac
    21841 gacctcgtgg tgaggttgcc gcaatgacgc atctggaact ggttcccgtc ccgcctgtcg
    21901 cgcaactggc gggcgtgagc cagcattatg gaaaaaacgt tgcgctgaac aatattactc
    21961 tcgatattcc ggcccgctgt atggtcgggc tgattggtcc ggatggcgtc gggaagtcga
    22021 gcttattgtc gttgatttcc ggtgcccgcg tcattgagca gggcaatgtg atggtgctgg
    22081 gcggcgatat gcgcgacccg aggcatcgcc gcgacgtctg cccgcgcatc gcctggatgc
    22141 cgcaggggct gggcaaaaac ctctaccaca ccttgtcggt gtatgaaaac gtcgattttt
    22201 tcgctcgcct gttcggtcac gacaaagcgg agcgggaagt gcgaattaat gagctgctga
    22261 ccagcacggg gttagcaccg tttcgcgatc gtccggcagg gaaactctcc ggcgggatga
    22321 agcaaaaact tgggctgtgc tgcgcgttaa tccacgaccc ggaactgttg atccttgatg
    22381 agccaacaac gggggttgac ccgctctccc gctcccagtt ctgggatctg atcgacagta
    22441 ttcgccagcg gcagagcaat atgagcgtgc tggtcgccac cgcctatatg gaagaggccg
    22501 aacgcttcga ctggctggta gcgatgaatg ccggagaagt gctggcaact ggcagcgccg
    22561 aagagctacg gcagcaaacg caaagcgcta cgctggaaga agcatttata aatctgttac
    22621 cgcaagcgca acgccaggcg catcaggcgg tagtgatccc accgtatcaa cctgaaaacg
    22681 cagagattgc catcgaagcg cgcgatctga ccatgcgttt tggttccttc gttgccgttg
    22741 atcacgttaa tttccgcatt ccacgcgggg agatttttgg ttttcttggt tcgaacggct
    22801 gcggtaaatc caccaccatg aaaatgctca ccggactgct gcccgccagc gaaggtgagg
    22861 cgtggctgtt cgggcaaccg gttgatccaa aagatatcga tacccgccgt cgggtgggct
    22921 atatgtcgca ggcgttttcg ctctataacg aactcaccgt gcggcaaaac cttgagttac
    22981 atgcccgttt gtttcacatc ccggaagcgg aaattcccgc aagagtggct gaaatgagcg
    23041 agcgttttaa gctcaacgac gttgaagatg ttctgccgga gtcattgccg ctcggcattc
    23101 gccagcggct ttcgctggcg gtggcggtga ttcatcgccc ggagatgtta atcctcgatg
    23161 agcctacttc tggtgtcgat ccggtggcga gggatatgtt ctggcagttg atggtcgatc
    23221 tctcgcgcca ggacaaagtg actatcttca tctctaccca ctttatgaac gaagcggaac
    23281 gttgcgaccg catctcactg atgcacgccg gaaaagtgct tgccagcggt acaccgcagg
    23341 aactggttga gaaacgcgga gccgccagtc tggaagaggc atttatcgcc tatttgcagg
    23401 aagcggcagg gcagagcaac gaagccgaag cgccgcccgt ggtacacgac accacccacg
    23461 cgccgcgtca gggatttagc ctgcgccgtc tgtttagcta cagccgccgc gaagcgctgg
    23521 aactgcgacg cgatccagta cgttcgacgc tggcgctgat gggaacggtg atcctgatgc
    23581 tgataatggg ttacggcatc agtatggatg tggaaaacct gcgctttgcg gtgctcgacc
    23641 gcgaccagac cgtcagtagc caggcgtgga cactcaacct ctccggttcc cgttacttta
    23701 tcgaacagcc gccgctcact agttatgacg agcttgatcg tcggatgcgt gcgggcgata
    23761 tcacggtggc gattgagatc ccgcccaatt tcgggcgcga tatcgcgcgt ggtacgcctg
    23821 tggaactcgg cgtctggatc gacggagcga tgcccagtcg tgccgaaacg gtaaaaggtt
    23881 acgtgcaggc aatgcaccag agttggcttc aggatgtggc gagccgacaa tcgacacccg
    23941 ccagccaaag cgggctgatg aacattgaga cgcgctatcg ctataacccg gacgtaaaaa
    24001 gcctgccagc gattgttccg gcggtgatcc cgcttctgct gatgatgatc ccgtcaatgc
    24061 taagcgccct tagcgtggtg cgggaaaaag agctagggtc gattatcaac ctttacgtga
    24121 cccccaccac ccgtagcgaa tttttgcttg gcaaacagct gccgtacatc gcgctgggga
    24181 tgctgaactt tttcctgctc tgcggcctgt cggtgtttgt gtttggcgta ccgcataaag
    24241 gcagtttcct gacgctcacc ctggcggcgc tgctgtatat catcattgcc accggaatgg
    24301 ggctgctgat ctccaccttt atgaaaagcc agattgccgc cattttcgga acggcgatta
    24361 tcacgttgat cccggcgaca cagttttccg ggatgatcga tccggtagct tcgctggaag
    24421 ggcctggacg ttggatcggc gaggtttacc cgaccagtca ttttctgact atcgcccgcg
    24481 gaacgttctc gaaagcgctg gatctgactg atttgtggca actttttatc ccgttgctga
    24541 tagccatccc gctggtgatg ggcttaagca tcctgctgct gaaaaaacag gagggatgat
    24601 gcgccattta cgcaatattt ttaatctggg tatcaaagag ttgcgcagtc tgctcggtga
    24661 taaagcgatg ctgacgctga ttgtcttctc gtttacggtg tcggtgtatt cgtcagcgac
    24721 cgttacgcca ggatcgttga acctcgcgcc gatcgccatt gccgatatgg atcaatcgca
    24781 gttatcgaac cggatcgtta acagcttcta tcgtccgtgg tttttgccac cggagatgat
    24841 caccgccgat gagatggatg ccggactgga cgccggacgc tataccttcg cgataaatat
    24901 tccgcctaat tttcagcgtg atgtcctcgc cggacgccag ccggatattc aggtgaacgt
    24961 cgatgccacg cgcatgagcc aggcatttac cggcaatggg tatatccaga atattatcaa
    25021 cggtgaagtg aacagctttg tcgcgcgcta ccgtgataac agcgaaccgt tggtatcgct
    25081 ggaaacccgg atgcgcttta acccgaacct cgatcccgcg tggtttggcg gggtgatggc
    25141 gatcatcaac aacattacca tgctggcgat tgtattgacc ggatcggcgc tgatccgcga
    25201 gcgtgaacac ggcacggtgg aacacttact ggtgatgccg ataacgccgt ttgagatcat
    25261 gatggcgaag atctggtcga tggggctggt ggtgctggtg gtatcgggat tatcgctggt
    25321 gctgatggcg aaaggtgtac tgggcgtacc gattgaaggc tcgatcccgc tgtttatgct
    25381 gggcgtggcg ctcagtctgt ttgccaccac gtcaatcggc atttttatgg ggacgatagc
    25441 gcgttcaatg ccgcaactgg ggctgctggt gattctggtg ctgctgccgc tgcaaatgct
    25501 ttccggtgga tccacgccgc gcgaaagtat gccgcagatg gtgcaggaca ttatgctgac
    25561 catgccgacg acacactttg ttagcctcgc gcaggccatt ctctaccggg gtgccggatt
    25621 cgaaatcgtc tggccgcagt tcctgacgct gatggcaatt ggcggcgcgt ttttcaccat
    25681 tgcgctgctg cgattcagga agacgattgg gacaatggcg taaggaaggg aaacttgact
    25741 tcatactgtg atgtcatact attgatgtca taaagtgaag tcataaggac atgccatgag
    25801 aaccaaggta caggctttac ggaagaaaaa aaaatacatt ggatcaaata tttaaaactc
    25861 ctgttcctca agggatcaag tggtctgata tagagtcact ggttaaagca ttaggcggag
    25921 aaattaagga aggaagaggt tcgcgttgta agttcatact aaatatgagc gttgcgtgtt
    25981 tccatcggcc tcatccgtcg ccagataccg ataaaggcgc tgtagaaagc gtgcgtgact
    26041 ggttactaag tataggggta aaaccatgat caaactgaag acgccaaact ctatggagat
    26101 tgccgggcaa ccagctgtta ttacctacgt gccagaactg aatgcatttc gtggcaaatt
    26161 cctgggcttg tcaggttatt gtgattttgt ctctgacagc attcaggggc tgcaaaaaga
    26221 aggggaactt tccctgcgag agtatcttga ggattgtaaa gctgcgggta ttgagccgta
    26281 tgcgcgtaca gaaaagatta agacctttac gttacgctat ccagaatcgc tgagtgaacg
    26341 ccttaataat gcggctgcgc agcaacaagt ctctgttaat acctatatta ttgaaacact
    26401 gaatgagcgt ttgaatcatt tataaatttt agcggcgtac attacgcaga atatctgcct
    26461 ttacaggatc tatctatgca actgaactta attttataaa ttaagttcag ttaatatctt
    26521 ttctttcctc gtttcagaaa taactcaatc ttccttcggc aagcactgca aatgcccatg
    26581 ccgcacgccg cgctgggcga taacgtcatc ggcaaaatgc tgcacgtcgc ccatatcacc
    26641 tttcagcacg gcgatttcca gacagtcgtc gtgattgata tgcacatgca gcgtggcgac
    26701 ggagagatcg tggtgatggt gctgggtgga gacaatgcgg ctggctaagt cgcgtttttc
    26761 gtgttcatac acataggaga gcaccgcgaa accttgcgtg ccgtgctgct gagtggcctc
    26821 ctgggccagg gcgctacgca gaatatcgcg gatagcttcg gaacggttgt tataaccacg
    26881 acgctggctc aggctgtcca gcgtctccag taaatcgtca tcaagcgtga tggtgactcg
    26941 ttgcatttgc gttaaacctt ttctgtggtg cgacggcgca cggggaatgc gggtaatacc
    27001 gcgttttgta gcacacgtcc ggcgtcagag gaaaaggtta atttatctcc caccacctgg
    27061 gtttcgacga tttgtccgtt gtccatcacc atcacccgct gacagaagcg ttccaccagg
    27121 cgtaagtcgt gggtgatgaa caggcaggcg gtgccaaact gttgttgtag ctttttcagc
    27181 aggcgaatga cgcccgcctg taacacgaga tcaaggttag aaacggcttc atccagaatc
    27241 agtagtttcg gttcgaccgc cagcgcgcga gccaggcaga cgcgctggag ctggccgccg
    27301 cttaactgcg gtgggcgttt gtcgagaacg ctgtcatcga gatcgaccgc cttcagcatt
    27361 tcgctggcgc gcgccagttg ttcggatttt ttcagtgata gcaggtggcg catcggttca
    27421 cgcaggatct cgcgcacggt tttgcgcgga ttcacggcgc tgatggagtc ctgaaatacc
    27481 atctgaatat cgcggcggaa cgctttacgc tgggcgcgat tgagtttcgc cagcggttcg
    27541 ccacgccagc taatattccc ctgcgcgggc gattctaaac ccaccagcaa ccgcgcgagg
    27601 gtacttttcc cgcagccgct gcgccccagc agggcgacgg tttcgccgct tttcagggtc
    27661 agggaaacgt tattcagcac cgcctgatgt tgatgttttc cgttaaatcc accgtgggca
    27721 tagtgatggg aaaggccgga gacgttaagt aaagtcatga tgccagctcc ataccgtaga
    27781 gggcgagatg agcggaaacc aggctgcgcg ttaccgcatg tttgggggcg ttaaacagcg
    27841 tttctacatc gccctgttcg acaattttac cttgtgacat caccgccacg tcatccgcca
    27901 gacgcgccac aacgcccata tcatgggtga ccagcagcat tcccggcgct tgtttttgca
    27961 taatgctttc cagcagatcg aggatgcgcg cctgtgctac cacgtcgagg tcggtggtcg
    28021 gttcatcggc gatgataaac ggtgattcac acagcaccgc catcgcaatc atcatgcgct
    28081 gcaacatgcc gccgctcatc tcgaacgggt acagcttcag cacgcgcgcg gcgttttcca
    28141 gccccaccgc ttctatggca gcggtaagcg tagcgtcatc ggcgggtttc cctaacgcca
    28201 ggcaggtttc ccgcgcgtgg gtgtgcatgg tgtgtagcgg attaaaggcg ctgcgcgggt
    28261 tctgcatgat ggtggcaatt ttgatgccgc gcagggcgca gggataaacc gctttgccat
    28321 cggctaaaat ttcccccgcc gtctggcgaa cgccagcggg caaaatgccc agcgtcgcgg
    28381 cgcaggttaa tgatttcccg ctgccgctac cgccgactaa cgccagcacg cgcccgcgtt
    28441 gcagggttaa cgatacaccg tgtaccagcg gctgcgcggc ctgtagcgcg atattacgta
    28501 gttcaatctg ttgcggcatt agtgtgcgtg ctccgtcacc agatgaggat ccagatgatc
    28561 gcgcagtgcg tcacccacca ggttaaaggc catcacgctg ataaacagcg ccagccccgg
    28621 ccagaacatt tgcagcggct gggtccagat atactggcgc gcgtcgttaa tcatcacgcc
    28681 ccattcggcg gtcggcgcgg tcacaccgag gccaaggaaa gacatccccg cgacgtgcag
    28741 catcatatgg ccgatatcca gcgttgccag caccagcagc gaagggatca ccgcgcctgc
    28801 cagatgatcg acaaacaccc gcacatggcc cgcaccggaa agccgtgacg ccagcacaaa
    28861 ctcgcgttgg cgtagtgaaa tcaccaagct gcgcaccatg cgtgcatacc acgcccagtg
    28921 cgacagggcg atggcgataa ttacgttggt cagcccggtg ccgagcacgc caaccataaa
    28981 gaacgacaga atcgaggtcg ggaaggtcat aaacatatcg gcgacgcgca tggtggcctg
    29041 atcaacgcgc ccgccaatca acccggcgct gccgccaata accagcccta atgtcagcac
    29101 cagcagcagg caggccatta ccgaaccgag cgacacgcgg gtcgctgcca tcagccgcga
    29161 gaaaatatcg cgacctaagt gatcggtgcc cagccagtgc tgcgcatccg gcgaaagcag
    29221 gcgcgacggc aaatcaatcg cctgggggtc atacggcagc caccactggc tggtgagcgc
    29281 aatcagcgcc agcagggcga taatgatcag cgccaggcgt accgaccagc gggaagagag
    29341 gaaaaagttc acgcgtgcgc tccttcatga cgacgaatgc gcgggtccag cgcggcgttg
    29401 agcaaatcga caatcaaatt acagaccaca aaaaccacca ccatcatcag cgtaaagcac
    29461 tgaatcaccg gatagtcacg gttaaaaatc gccgacaccg catagcgccc gacgcccggc
    29521 caggcaaaga tgttttcgat aatcatcgtc ccgccaatca gttcgccgat gtgcatcccc
    29581 acggcggtga tcatcggcag cgaggcattg cgcagaatat ggcggcgttc ggtctgtttg
    29641 tcgttcaggc cgcgcagacg cgcccaagtg acgtgacgct gaccggcgac gtccagcata
    29701 ctggcgcgca gcagtcgcgc gttaatcgcc agcgacataa aggcaatgga aaccgcaggc
    29761 aaaatgatgt gctgccagcc gccgtaaccc atcgcgggta gccattgcag atacaccgaa
    29821 aacgccatca ccagtaaaaa cgccagccag aagttaggca tcgacacgcc aagaaacgcg
    29881 atgaaacgta cggcgaaatc cggcagacgg tcgcgatggc gcgccgccca gataccgagc
    29941 ggtacggaag tgagcagaat taataccagc gccgcacctg caagttccag cgtggcgggc
    30001 aggaagttca gcatatcgtc cagcaccggg cgttggctgg cgaatgagat accaaagtca
    30061 agatgcagcg ccttccacaa ccaggtaccg tactggacgt acagcggctg atccagcccg
    30121 agcatggtgc gggtagaggc cagcatctcc ggcgtcggcg gcaggttaga taaacgcaga
    30181 taatcgagcg ccgggtcgcc ggtaccgagg cgcagcatca gaaaaatgat caccgaggcg
    30241 gcgagcacca tcgggatcag cagcagaaag cggcgtaata cgtaacgcaa cattaaggtt
    30301 tcaccggttt aatctgttcg aacggaattt cggtggcgat cggcgcgtag gggatattac
    30361 ccagctccgg ttttgatacc accatcatcg agatgtagct gataggcaga taaaccgcct
    30421 cgtcatgcag acgggtcaaa atgtcgcgat acagcgcctg acgttgcgtt tcgtcatggg
    30481 tcgccagcac ttcgccgatc tctttatcaa tcagcggctt gtcggctaat ccttgctgtg
    30541 cctggaagtc agcgtgcgac ggtacgcgca ttgaactcag gaaggcgtgt ggatcgtatg
    30601 gcgcgcccca ggtgcggtgg aaaatcatgc caaaacgacc gtcgcgctga cgagcataga
    30661 tactgctctc ttcttcgcca atcagcgaga catctgcgcc aatctggcgc atatcagcct
    30721 gaatgatttc cgccatcgat ttgcttaacg catcggtgcc gatgaacgac agttcaatgc
    30781 gcagcggctg accatttttc tcgcggatgt ctttgcccgc aggcagcgtc caaccggctt
    30841 tttccagcaa ctctttcgct ttctgcggat cgtactggcg cggtttcagg ccgagattag
    30901 cgtaaggcac agaaggggca aacagggtgt cggcgacctg cggggtgcca tataacgcgt
    30961 tatcaatcag cgattttttg tttaccgcgt aattaagagc ttcacgtact gccagctcgt
    31021 tggtgggggc tttggcggta ttgagcgcca gcatcacggt ttcgatcggc tgtgacagtt
    31081 gggtgtggta agccggattc tggctaaagc gggcgaaggt atcgagcggt aataaccctt
    31141 cgtttccgta cagcaggtcg atatcgccag tttcaaacgc caccgcgcgg gtagtcgggt
    31201 ccgggatgac gttaaaggtg atctttttaa tcgctggctt ttcgccccag tagttttcgt
    31261 tacggacgaa gacatcgtac tgattcagtt tcgattcctg caaaatccac ggtccggtgc
    31321 caatcggcgc tttaattccg ttcatggttt catggttttt aaactgcgag ggagcgataa
    31381 aacggaaagg acggggcagg gccagttctt gcaggaaagg atagtaggcg cttttcaggg
    31441 taatttgcag ctctgtttta ctgagtgctt taacatcaac aatctggttt gccagctcca
    31501 gccaggcgtg acgttgacgg ttatcgagca ctgcgcggaa gttttctgcc gccgcctcgg
    31561 catcgaacgg ttcaccgttg gagaatttca cgtcatcacg cagggtgaag gtccaggttt
    31621 taccatcttc tgaatgagtc cagctttttg ccagccacgg gatcaccgaa ccgtctgcct
    31681 gatatttcac caatggttca taaaccatgc tctgggcgaa catctggtta ggcgtgtaaa
    31741 ggtgcgggtt tagtggcccg acattcaccg gccaggcggt agtgatttca tctggtgcag
    31801 cggcatggac gataaaagac gcacaagcca gcagcgcaaa tagagtgcgg cggagtgtgg
    31861 agagcatggt aaccccaatg gattaaaata gatggcggaa ataagtatga cgattttaag
    31921 tattcgtcat actgattacc tgttctggat caatagatgg gcagtcgggt gggcggatca
    31981 gttaactgaa tcgatccatt gcacactgtc ggcggtgagc gtaaaggggg tgggggtgca
    32041 gatcgcaagg cttaaatttt ctagctgaca atggctgact gacagcgagg agtgataggt
    32101 gctatctacg ctgacgattt gccaggcgct gccgccacgc tgtttaacga tggcttcttt
    32161 gcgcgtccag atgcgccaga acatttccag ttgctgatca ggatgcacgg cgtccatttc
    32221 agcgtgttcc ccgaggctga acacggcgtt cgccagccag cgccagttgg cgcgcgggcg
    32281 aatcacttcg atatcgcagc cgacttcgcc ttcatcgctc aacagcagag cgatatcgtc
    32341 accgctatgg cttaagttga accatagcgg cgtttccggc gcgaatgcag gtttgccttg
    32401 ttcgccatag atgatctccg gtagcgggga aagcgtgtgc gaaagcaatg cacgccccgc
    32461 cagccagcgt tcgcgtcgtg gaccttgcgg tgcttgctcg cgtaaacccg gtggcagtgg
    32521 agctgcgctt aaggtcgaaa ctttccccag aactatccga tacatatcag ggccaacgtt
    32581 taatggaaaa tgaaagtgcg tatcgtatca cttgtcgcct catcccggta accgactttt
    32641 cggtctgcct ggccccagta aaatcgccag tttgctaccg cctttggtgg tttccatcca
    32701 gattttacac acactggtta atggcaccga aagtagcatc cctaccgggc cgagcagcca
    32761 tccccaaatt aacaatgaaa gaaataccac catggtggac atccccaggc gatggcccat
    32821 catccgtggt tctaaaatat tgccgatgac catatggacc actaaaaaca atgcgccgac
    32881 cagaatacat tcgtaaacac cattaaacag cagcacctga atcattggcg gtacggcgga
    32941 aattaccgcg ccgatattgg gcacgtagtt gagcaaaaac gccagtactg cccacatcag
    33001 cgcaaactgc acccccatca gctccagccc cagccagacg atgacacctg tccataaact
    33061 gagtagcgtc ttcaatgcaa gatagtgcga aacgccttta agtgcgcggt gtaatcctgc
    33121 gatgtgaatc tgtgggttat tcagcgcaaa acgcattttg taagggacgt ggcgcacttc
    33181 aaacagcata aaaactacgg tcatcaccag caaaagcacg ctcgccattg ccccggaaag
    33241 cccggtcatt agcgctgtgg tgaaggtaac cactttttcc gagtccatcc gctgcagcat
    33301 tcgctccggc gacatatgca aattaagaaa aggcaacatc tcctgcaatt taaaaagttt
    33361 gcgcgtcagc tccttattaa acttcggcag catagagata aattcgttaa acgatgccgc
    33421 cagtacgccg accagcgcgg ttagtgcgat cagcatcacc accactacaa tcgtaatggc
    33481 aacggggcgt tgtactcccc gacgaataaa ccaggtaacg agcgggttga ggacgatggc
    33541 aaaaaacagc gccagtaaca gctgcacaat gatatctgcc gctgcgtgaa tgcccgcgag
    33601 gatcactacc agcgaggcca gcttgagcag aatgtgcatg cccgttttat cgggttgagg
    33661 gatttccatt ggggcttcct tgtgactttt tgtattaagt gtagcgggag tcacgccagc
    33721 aatattctga ttcttactgc tgattttcac gtcagcacgt ggtaaaaatg aaacactgtt
    33781 gtaaaaatgt ggtgatcctc atgcccgaac ccgtagccga acccgcgcta aacggattgc
    33841 gcctgaattt gcgcattgtc tccattgtca tgtttaactt cgccagctac ctcaccatcg
    33901 ggttgccgct cgctgtatta ccgggctatg tccatgatgt gatgggcttt agcgctttct
    33961 gggcaggatt ggttatcagc ctgcaatatt tcgccacctt gctgagccgt cctcatgccg
    34021 gacgttacgc cgatttgctg ggccccaaaa agattgtcgt cttcggttta tgcggctgct
    34081 ttttgagcgg tctgggatat ctgacggcag gattaaccgc cagtctgccc gtcatcagcc
    34141 tgttattact ttgcctggga cgcgtgatcc ttgggattgg gcaaagtttt gccggaacgg
    34201 gatcgaccct gtggggcgtt ggcgtggttg gctcgctgca tatcgggcga gtgatttcgt
    34261 ggaacggcat tgtcaattac ggggcgatgg cgatgggtgc gccgttaggc gtcgtgtttt
    34321 atcactgggg cggcttgcag gcgttagcgt taatcattat gggcgtggcg ctggtggcca
    34381 ttttgttggc gatcccgcgt ccgacggtaa aagccagtaa aggcaaacca ctgccgtttc
    34441 gcgcggtgct tgggcgcgtc tggctgtacg gtatggcact ggcactggct tctgccggat
    34501 ttggcgtcat cgccaccttt atcacgctgt tttatgacgc taaaggttgg gacggtgcgg
    34561 ctttcgcgct gacgctgttt agctgtgcgt ttgtcggtac gcgtttgtta ttccctaacg
    34621 gcattaaccg tatcggcggc ttaaacgtgg cgatgatttg ctttagcgtt gagataatcg
    34681 gcctgctact ggttggcgtg gcgactatgc cgtggatggc gaaagtcggc gtcttactgg
    34741 cgggggcagg gttttcgctg gtgttcccgg cattgggcgt agtggcggta aaagcggttc
    34801 cgcagcaaaa tcagggggcg gcgctggcaa cttacaccgt atttatggat ttatcgcttg
    34861 gcgtgaccgg accactggct gggctggtga tgagctgggc gggcgtaccg gtgatttatc
    34921 tggcggcggc gggactggtc gcaatcgcgt tattactgac gtggcgatta aaaaaacggc
    34981 ctccggaaca cgtccctgag gccgcctcat catcttaaaa cttactgaat aaccagcgta
    35041 ttaatgatgt tttctgcggt ggtctgcgct ttttgctgat cgtcagctgg cagagtaatt
    35101 tgcatggtca gcagttgatt acccacgtta cccagaataa cggaagagta cgccgtctgg
    35161 cctttcgcgg agataatact gtctaactgc tgcattttgt gacctttcag ctcaatggct
    35221 ttgttggtta ccacttgcag ctgcggatcg cggctacgtt gctgatcttc cagacgcttc
    35281 gccaacaccg ccagatcttc tttcggatca tcgcccatga tgacgatgac tgctttctgc
    35341 ccggtggcgt cggaccagac atgcatgtta ttggcctgcg ttcccagctt accgctctgg
    35401 tcggtcatat ccgctggcag cgagaaactt aacttgccat caagcaggtt gacgggattc
    35461 ccggtagcgt tactttccgc gaccgaaccc tgcgccgtag cgttagtgtc tttatcatca
    35521 caggccgcaa gccccataac cagcaggcca attccgacat atttaaccag attgcgcatt
    35581 gacttcttcc tttcgataaa cggccataac ggctcattca tccatcttat cacaactctg
    35641 ataacgaacc tttaactcgc ctgcaaagcg ttgatttcgg atttatctgc cagtcttttc
    35701 aacagaatat tgagtaatac gccatacatt ggcaggaaga aaacgatact gattaacact
    35761 ttgaaacagt aatcgaccag cgcgatttcc atccagtgtt cagccataaa ggcatccggg
    35821 ctacgccaga aggcaatgaa gaaaaaggcc agcgtgtcgc tgacgttacc gaacagtgtg
    35881 gacgctgtcg gtgccagcca ccagcggcga ctctgacgca ggcggttaaa aacgtgcacg
    35941 tcgaggatct gtcccagcgc gtaggccatg aaactggcgg tggcgatacg ggcgacaaac
    36001 aggttgaagt gggcgagtgc gccgaatccc tgccaggaac ccatatagaa tagcgacgag
    36061 atgacgtagg agatcagcaa cgccgggatc atcaccgcaa agatgatacg acgggccagc
    36121 ggtgcgccaa aaatacgcac ggtcaggtcg gtagcaagga aaataaacgg gaagctaaac
    36181 gcgccccagg tggtatggaa acccaaaatg gagacgggaa gctgaaccag atagttactg
    36241 gaggtgatca ccagcagatg aaataacgat aaccagaaca acgccttata gcgttgagtt
    36301 tgcgagaaaa cgttcatatt gtaccttttt gattaaccat tggggtgagg gaacccaata
    36361 cgtacgacac gtctgttacc ttaacataat aacgtgctta cccttttttc gagccgccgc
    36421 atgatactgc tttacgttga caatgcaatg gttaattttc acgcaatcgt taacctggtt
    36481 tgcttacgga ctcacagggc gtaaactagc gccgtttttt tatgtgatga gaagaaaatg
    36541 accgatctct tttccagccc tgaccacaca ctcgacgcac ttggcctgcg ctgcccggaa
    36601 cccgtaatga tggtgcgcaa aactgtgcgc aatatgcagc ctggcgaaac gttgctgatt
    36661 atcgccgacg atccggccac tacccgcgat attcctgggt tttgtacctt tatggaacac
    36721 gaactggttg cgaaagagac ggatggactg ccttatcgtt atttgattcg taaaggcggt
    36781 tgatggggaa agattggctt cgatgccgcc ttttcccctc accctaaccc tctccccaga
    36841 ggggcgaggg gaccgatcgc gctcaatgtt gcgatcggtt tgccttatct cctgcgcaac
    36901 aatcttaacg cattcgctgt caccagcacc gtcgcacccg tatctgccag caccgccagc
    36961 cacaggccgg tcatccctaa cagcgtggtg acgaggaaga tccctttcag ccccagcgca
    37021 atggtgatgt tctggcggat attggcgtga gtggcgcgtg ccagttcaat catttgcacc
    37081 aggccgcgca ggtggttatg ggttaatgct gcatcagcgg tttccagcgc cacgtctgtg
    37141 ccactgccca ttgcaatccc aatggcggca gctttcatcg ctggcgcgtc gttaatacca
    37201 tcaccgacca tcgccagggg cgcatgttga ttcagcttgg tcaccgcttt gactttatct
    37261 tccggcaata ggcccgcttt aaactccagc cccagctccc cggcgattgc cgccgctgca
    37321 cgtggattat cgccggtgag gatcactcct ttgaccccca gcgcgttcag ttcactgatg
    37381 gcagttgcag catcggcgcg cagggtatcc tgcaatgcaa tgatacccag cacgtcatca
    37441 ttacgtacta ccagcacaac cgtttgcccg gcgctttcca gttcattaat caaaccagca
    37501 aatgcatcag cgggatgttt cccggcagcg caaatcaata cgcgctcacc gttaacctgc
    37561 gcttcaatgc cagacccgac cagcgcccgc tgtgattcgg cggtgggaat ggcgagttca
    37621 gcaacctgtg cttcgcgtac gatggcttgc gccagtggat gcgtcgcgcc ttgctcgacc
    37681 gccgccgcca gtgtcagcag ttcagattca ctaatacccg ttgccggatg aatcgcggta
    37741 acgcgcggtt taccgacggt cagcgtaccg gttttatcaa acgccacctg agtaacacga
    37801 cctagttgtt ccagcgccgc tccgccttta atcaacgccc cacgacgcgc tgccgccgcc
    37861 agcccggagg taatcgccgc aggcgttgag ataactaacg cacacgggca gccaatcagc
    37921 agcagcgtca gccctttata aatccactcc tgccagctgg cggcaaacag cagcggtggc
    37981 accagcgtca ccagcagagc gacggccata atcgcgggcg tatagatacg gctgaaacgg
    38041 tcgataaacc gctcaatggg ggcgcgacgc tcttcggctt cttcgatcag tttcagaatc
    38101 cggtcaatgg cgctggcacc cggttctgac agcacttcca gcgttaccag acggtctacg
    38161 ctggtggctc ctgcaggaac tttatcgccc gtcgcgcgct ccaccggaat ggattctccg
    38221 gtcagggcgc tttcatcaaa actggcaaac ggtgagagca gtttaccgtc ggcaggcaaa
    38281 cgcccacctg cggcgacttc aatcacatcg ccaggccgca ggctgttaat cgccacctct
    38341 tcccgctcac cgttacgcag gcgcgtggcg gtttctggtt tcagcgccat taacgcgcta
    38401 acgccctgac gcgcgcggct ggcggcccag ccttccagtc gttcaccaat caaaaacagc
    38461 agcaacacca tcgcagcttc agccgttgcg ccaataaaca gtgcaccaat agcggctacg
    38521 ctcattaagg tttcaatggc gaagtagctg ccggatttga tcagccgtaa tgcctgacga
    38581 gcaatcgggt acagcccaac cagcgtggtc gcgataaacg ccagttgccc gaacggatga
    38641 ttgaactgct ccagacccca gctgattgcc atcatcacga ttagtgtaat cagcggcaga
    38701 ttctctttca ggcgcgatgc ttgtggctcg tcggcggcct gctcatcgcg cagggaatag
    38761 cccgcttttt gcaccgcaga ttcaacttgt gcacgaatgt cattgtcggc atcaaccacc
    38821 agtttttcgg tggcgaacaa cacctgcacc tgattcacac ctgcaagctg gcgcacggca
    38881 ttttctacct tgcgcgcaca ggcggcgcag tccatgccac tgactttcca gctatagcgg
    38941 gtgccggaga cgttttcaga gagcgttggc gagctggagc atgcgccgtc gcagcaacag
    39001 tcgttggcgt tctgtaccgt ggttagcggt ttgaacgcag caaattgagg ggctttcttg
    39061 ccgtgattgt caggtgtcga catggcatac tccggttaag ttttttctca ttaaccgaag
    39121 gatacactct ggagtcgact ccagagtcaa gttttatcag agatacagtg agcggatgat
    39181 gaggaagtga ccggcaaagt agcaggccgc agcaatcgcg ttatccgcac ggaagcgtcg
    39241 gcgatagtgg ctccccagcc agacaaagtt actgataaac agcaacgaag cgccaacaaa
    39301 cgcagagagc gccggagcgg tcggacggaa gaaccacagt tcacctgcca gccacaccat
    39361 caccagcgtc atgccgataa acgtgcagat aggccaacga tactcttcca gccgcgtcca
    39421 gataatcgcc agtaacagcg cacccagaac cagcaacacc agtggcagag gccagaagaa
    39481 agagagcgtc atctgactgg cgaaatagat ggtgtacagc aggtgcgaga ggaaaaacgc
    39541 gccgatggcg tacatcagac gttgacgtgg caacagggtt agcgcatcgc ccagcagtga
    39601 ggcgcacagc cctgccagca ccagatagct aatggcgtcg aacatcggcg cttgccaggc
    39661 cagtaacagc aggagaagaa gggttaacgg tttaaacacc cagcgttgcc aggttggccc
    39721 acgatacgat gcatccacag atagccatgc ggaaagacag acagcgataa acgaccaaag
    39781 catcttagtt ccttgaattc gttatttttt cgtaagcgga atgcctacgg tcttgcttcc
    39841 agtttagtgg cgagggtgga cggatgacaa caccataact ggcagggtat gcttatttcc
    39901 gcattttccg atgagggata aagatgagca aaccacctct tttctttatt gttatcattg
    39961 gcttaattgt cgtcgccgca tcgtttcgtt ttatgcaaca gcgacgggaa aaagctgata
    40021 atgatatggc tccgctccag caaaagctgg tggtggtgag caacaagcgg gaaaaaccga
    40081 ttaacgatcg ccgttcgcgc cagcaggaag tgactccggc aggcaccagt atgcgctacg
    40141 aggcaagctt caaaccgcaa agcggaggaa tggagcagac gtttcgcctc gacgcccagc
    40201 agtaccacgc cctgacagtg ggcgataaag gtacgctgag ctataaagga acgcgctttg
    40261 ttagctttgt tggcgaacaa taaggttatt ttttaacttt aaatttcttc tgccagacca
    40321 gcagttcaaa cacgccgaaa aggaaaatcc gtaccttttc ggcggtggtc atctgcgggc
    40381 catctttcgg taaagtggat ttcaatagcg ccagctgcat accatgcatc agcacggtaa
    40441 aaatcagcgc cacgttaacg aaaatattta gcgggcgcgg gaagggatgc accagattga
    40501 ggattaaaaa tccccaaacg cagagcatta acaaacgacc aatattaatc agcatcactt
    40561 tctccttgtg cttcgcgttg atacagccga taagccacct gacccgccac tttttcccga
    40621 tgtaacgacc agtttgccgg aacggtgggc agaccgtttt cgacttcgct ttcgacataa
    40681 atcagggctt cgtcagccag ccagccgtta tcttccagta aatttatcgt ctcttctaac
    40741 aagccacggc ggaacggtgg atcgacaaac acgatattat gcggtgtgcc tttttgcgcc
    40801 aggaatgaca tcgcgttgct gttcaccacg cgtgcattgc ctgcttttag tgtcgccaga
    40861 ttcttaatta actgctgaga aactgcacga tccatctcaa ttaacgtggc ccccgcagcg
    40921 tagcgcgata acgcttccag ccccagcgcg ccgctcccgg cgaagcaatc cagacattgg
    40981 gcgtcaacaa tgaccggagc cagccagtta aacaacgttt cgcgtacgcg gtcggtggtg
    41041 gggcgcagac ctgggctatc aggaaccggg agtttacggc ctcgccactg cccgccaata
    41101 atgcggattt ggccgctgcc ggaatgattc ggttttttca tgataaattg ctcaatccgc
    41161 cagataacac atacttgcag gcggtgatgt gaattaagtt aagtgataga ctatttcatc
    41221 atttttttag ctgctatgta catagcgtta acgctgtgcc atgaagcaac agcgaggagt
    41281 gtagtcgcaa atggcgaaag aaaaaaaacg tggctttttt tcctggctgg gctttggtca
    41341 aaaagagcag accccggaaa aagagacaga agttcagaat gaacaaccgg ttgtagaaga
    41401 aatcgttcag gcgcaagagc ctgtgaaggc ttcggaacaa gccgttgaag agcagccgca
    41461 ggcgcatact gaagccgagg cggaaacttt tgctgccgac gttgtggaag tcactgaaca
    41521 ggttgttgaa agtgaaaaag cgcagcctga agcggaagtt gttgcacagc cggaaccggt
    41581 cgtagaagaa acgccggagc cagtggctat cgaacgtgaa gagctgccgt tgccggaaga
    41641 cgtcaacgcc gaagaggttt cgccagaaga gtggcaggct gaagcggaaa ccgtagagat
    41701 tgtcaaagcg gcggaagaag aagcggctaa agaagaaatc accgacgaag agctggaagc
    41761 acaggcgctg gcagccgaag cagcagaaga ggcggtgatg gtggttcctc cggcagaaga
    41821 agagcagccg gtggaagaaa tcgctcagga gcaggaaaaa ccaaccaaag aaggtttctt
    41881 cgcgcgcctg aaacgcagcc tgttaaaaac caaagaaaat ctcggttccg gatttatcag
    41941 cctgttccgc ggtaaaaaaa tcgacgatga tctgtttgag gagctggaag agcagctttt
    42001 gatcgccgat gtgggcgtgg aaaccacacg taaaattatc accaatctga cggaaggcgc
    42061 atcccgcaag cagcttcgtg atgccgaggc gctctatggc ctgctgaaag aagagatggg
    42121 tgagattctg gcgaaagtcg atgagccgct gaatgttgaa ggtaaaacgc cgttcgtgat
    42181 cctgatggtg ggcgtcaacg gtgtgggtaa aaccacgacg attggtaagc tggcacgtca
    42241 gtttgagcag cagggtaaat cggtgatgct ggcggcgggc gatactttcc gtgcagcagc
    42301 ggttgaacag cttcaggtct ggggtcagcg caacaatatt ccggtgattg cccagcatac
    42361 cggggcggat tccgcctctg ttatcttcga cgccattcag gcagctaaag cgcgtaatat
    42421 cgacgtcctg attgccgata ccgccggacg cctgcagaac aaatcgcacc tgatggaaga
    42481 gttgaagaaa atcgtccgcg tgatgaagaa actcgacgtt gaagcgccgc atgaagttat
    42541 gctgactatt gatgccagca ccgggcagaa cgcggtaagc caggccaaac tgttccacga
    42601 agctgttggc ttaaccggca tcacgctaac gaaactggac ggcacggcga aaggcggggt
    42661 aattttctcg gtggctgacc agtttggtat ccctatccgc tacattggtg tcggcgaacg
    42721 tattgaggat ttgcgtccgt ttaaggcgga cgactttata gaggcacttt ttgcccgaga
    42781 ggattaacaa tgattcgctt tgaacatgtc agcaaggctt atctcggtgg gagacaggcg
    42841 ctgcagggcg ttacgttcca tatgcagccg ggtgagatgg cgtttctgac cggtcattcc
    42901 ggcgcaggga aaagtaccct cctgaagctg atctgtggga ttgagcggcc cagcgccggg
    42961 aaaatctggt ttagcggcca tgacatcacg cgtctgaaaa accgtgaagt tccgtttctg
    43021 cgccgccaga ttggcatgat tttccaggat caccatctac tgatggaccg tactgtctac
    43081 gataacgtgg cgatcccgct gattatcgct ggtgccagcg gtgacgatat tcgtcgccgg
    43141 gtgtcggcgg cactggataa agtcgggcta ctggacaaag cgaagaactt ccctattcag
    43201 ctttccggcg gtgaacaaca gcgtgttggc attgcccgcg cggtggtgaa taagcccgcg
    43261 gtactgctgg cggacgaacc gaccggtaac ctggacgacg cgttgtcgga agggattcta
    43321 cgtctgtttg aagagtttaa ccgcgttggg gtaaccgtat tgatggcaac gcacgacatc
    43381 aacctgattt cacggcgttc ccatcgcatg ctcaccctga gcgatggtca cttgcatgga
    43441 ggcgtgggcc atgaataagc gcgatgcaat caatcatatt cggcagtttg gtgggcgtct
    43501 ggatcgcttc cgtaaatcgg tcggcggctc aggcgacggc ggtcgtaacg caccgaaacg
    43561 cgcgaaatcc tcgccaaaac cggtaaatcg caaaaccaac gttttcaacg aacaggtgcg
    43621 ctatgccttc cacggcgcat tgcaggatct gaaaagcaaa ccgttcgcca cgtttttaac
    43681 ggtgatggtt atcgccatct ccctgacgct gcccagcgtc tgttatatgg tgtacaaaaa
    43741 cgttaatcag gcggcgacgc agtattatcc gtcaccgcaa atcactgttt acttgcaaaa
    43801 aacgctggac gatgacgccg ctgcgggcgt ggtggcacag ttgcaggccg agcaaggcgt
    43861 ggagaaagtg aactatcttt ctcgtgaaga cgcactgggc gagttccgta actggtctgg
    43921 ttttggtggt gcgctggata tgctggaaga aaacccgctt ccggcagtgg cggtggtgat
    43981 cccgaaactc gattttcagg ggacggaatc actcaatacg ctgcgtgatc gtatcacgca
    44041 gattaacggc attgacgaag tgcggatgga tgacagctgg tttgcccgtc tggcggcgtt
    44101 gaccgggctg gtcgggcgcg tttcggcgat gatcggcgtg ttgatggtgg cggcagtgtt
    44161 cctcgtcatc ggcaacagcg tacgtctgag catctttgct cgccgtgact ccatcaacgt
    44221 gcagaaactg attggtgcga cggatggttt catccttcgc ccgttcctgt atggtggcgc
    44281 actgctggga ttttctggcg cattgttgtc attaattttg tcagaaattc tggtgctgcg
    44341 attgtcatcg gcggttgcgg aagtggcaca ggttttcgga acgaagtttg atatcaatga
    44401 tttatcgttc gatgaatgcc tgctattgct gctggtatgc tcgatgattg gctgggtggc
    44461 agcgtggctt gccacggtac aacatttacg ccactttacg cctgaataat aaaagcgtgt
    44521 tatactcttt ccctgcaatg ggttccgtag cagggaaaga gatcccgttg tctcttcccg
    44581 gtatttcatc tctatgtcac attttgtgcg taatttattc acaagcttgc attgaacttg
    44641 tggataaaat cactgtctga taaaacagtg gatgataacc tcgttgctct taagctctgg
    44701 cacagttgtt gctaccactg aagcgccaga agatatcgat tgagaggatt tgaatgactg
    44761 acaaaatgca aagtttagct ttagccccag ttggcaacct ggattcctac atccgggcag
    44821 ctaacgcgtg gccgatgttg tcggctgacg aggagcgggc gctggctgaa aagctgcatt
    44881 accatggcga tctggaagca gctaaaacgc tgatcctgtc tcacctgcgg tttgttgttc
    44941 atattgctcg taattatgcg ggctatggcc tgccacaggc ggatttgatt caggaaggta
    45001 acatcggcct gatgaaagca gtgcgccgtt tcaacccgga agtgggtgtg cgcctggtct
    45061 ccttcgccgt tcactggatc aaagcagaga tccacgaata cgttctgcgt aactggcgta
    45121 tcgtcaaagt tgcgaccacc aaagcgcagc gcaaactgtt cttcaacctg cgtaaaacca
    45181 agcagcgtct gggctggttt aaccaggatg aagtcgaaat ggtggcccgt gaactgggcg
    45241 taaccagcaa agacgttcgt gagatggaat cacgtatggc ggcacaggac atgacctttg
    45301 acctgtcttc cgacgacgat tccgacagcc agccgatggc accggtgctc tatctgcagg
    45361 ataaatcatc taactttgcc gacggcatcg aagatgataa ctgggaagag caggcggcaa
    45421 accgtctgac cgacgcgatg caaggtctgg acgaacgcag ccaggacatc atccgcgcgc
    45481 gctggctgga cgaagacaac aagtccacgt tgcaggaact ggctgaccgt tacggtgttt
    45541 ccgctgaacg tgtacgccag ctggaaaaga acgcgatgaa aaaactgcgc gctgctatag
    45601 aagcgtaatt tccgctatta agcagagaac cctggatgcg agtccggggt ttttgttttt
    45661 tgggcctcta caataatcaa tttcccctcc ggcaaaacgc caatccccac gcagattgtt
    45721 aataaactgt caaaatagct attccaatat cataaaaatc gggtatgttt tagcagagta
    45781 tgctgctaaa gcacgggtag ccatgcataa aacgaaataa agtgctgaac aacaacatca
    45841 caacacacgt aataaccaga agaatgggga ttctcaggat gaacataaag ggtaaagcgt
    45901 tactggcagg atgtatcgcg ctggcattca gcaatatggc tctggcagaa gatattaaag
    45961 tcgcggtcgt gggcgcaatg tccggtccgg ttgcgcagta cggtgaccaa gagtttaccg
    46021 gcgcagagca ggcggttgcg gatatcaacg ctaaaggcgg cattaaaggc aacaaactgc
    46081 aaatcgtaaa atatgacgat gcctgtgacc cgaaacaggc ggttgcggtg gcgaacaaag
    46141 tcgttaacga cggcattaaa tatgtgattg gtcacctctg ttcttcatca acgcagcctg
    46201 cgtctgacat ctacgaagac gaaggcattt taatgatcac cccagcggca accgcgccgg
    46261 agctgaccgc ccgtggctat cagctgatcc tgcgcaccac cggcctggac tccgaccagg
    46321 ggccgacggc ggcgaaatat attcttgaga aagtgaaacc gcagcgtatt gctatcgttc
    46381 acgacaaaca gcaatacggc gaaggtctgg cacgagcggt gcaggacggc ctgaagaaag
    46441 gcaatgctaa cgtggtgttc tttgatggta tcaccgccgg ggaaaaagat ttctctacgc
    46501 tggtggcgcg tctgaagaaa gagaatatcg acttcgttta ctacggcggt taccacccgg
    46561 aaatggggca aatcctgcgt caggcacgcg cggcagggct gaaaactcag tttatggggc
    46621 cggaaggtgt ggctaacgtt tcgctgtcta acattgcggg cgaatcagcg gaagggctgc
    46681 tggtgaccaa gccgaagaac tacgatcagg ttccggcaaa caaacccatt gttgacgcga
    46741 tcaaagcgaa aaaacaggac ccaagcggcg cattcgtttg gaccacctac gccgcgctgc
    46801 aatctttgca ggcgggcctg aatcagtctg acgatccggc tgaaatcgcc aaatacctga
    46861 aagcgaactc cgtggatacc gtaatgggac cgctgacgtg ggatgagaaa ggcgatctga
    46921 aaggctttga gttcggcgta tttgactggc acgccaacgg cacggcgaca gatgcgaagt
    46981 aatcattaat cggcaacttt gggttgccgc caaattgcta atatcgagta cgttgcttca
    47041 tgccggatgc ggcgtaaacg ccttatccgg cctataagat ccaaagaaat cagtaaattg
    47101 caacacacat tgtaggcctg ataagcgtag cgcatcaggc aatttctcgt tggtcatcac
    47161 ttttgaaatc aacttgacga ttaacgcttc tcccagccgc cctgttgcgc cgtaaatccc
    47221 agtgcctgca taaacgctgt catcacaccg cgatcttcca cgcctgcatc cgccatccac
    47281 cagcatgaaa cgccaggatt gttacgcaaa acctcttcca gcagatattg ccccacaccg
    47341 cgacgtcggg tgacttcccg cacacgcagg gaatccagtg ctccctcggt gccgcttaag
    47401 gttacccgta cggcagcgag caggcgctcg ttaaaacgcg cggcgtagat acggtggtta
    47461 tcgtcaacct gtaaagagga aggggaatac tccggccaga tcttttgcag gtcaatccgg
    47521 tcctggtcgc taaagttttc taatcgaatg atggtcagct tcatgggtaa cccgtgtaaa
    47581 tcacaaaagt ggaaccagtg tagcgaaata atttaatcgg aggctttctc ttttttattt
    47641 cttttggcag gtgattaatt ttttaacagc aataattaca aagttaaaac attagagaat
    47701 gaaaaatacc cagcataatc ccctgaatgg tagtgaatta ttccgccctt tgtgccgtta
    47761 ttttatgctg acaaaggcac ttttttctgt ttatctatca ataaattcag aatattatct
    47821 gttcttaatc gactgaaaaa tagggatttt aatcactgtt atcacaaaat actgcgctaa
    47881 acccttaatc agacaggcaa aaacagtgca gtataaaaaa agaacagtct gatttgttaa
    47941 cacataaaaa caaagcaaca caacatcacg aatggggatt tttgactatg aaacggaatg
    48001 cgaaaactat catcgcaggg atgattgcac tggcaatttc acacaccgct atggctgacg
    48061 atattaaagt cgccgttgtc ggcgcgatgt ccggcccgat tgcccagtgg ggcgatatgg
    48121 aatttaacgg cgcgcgtcag gcgattaaag acattaatgc caaaggggga attaagggcg
    48181 acaagctggt tggcgtggaa tatgacgacg cctgcgaccc gaaacaagcc gttgcggtcg
    48241 ccaacaaaat cgttaatgac ggcattaaat acgttattgg tcatctgtgt tcttcttcta
    48301 cccaacctgc atcagatatc tacgaagacg aaggtattct gatgatctcg ccgggagcga
    48361 ccaacccgga gctgacccaa cgcggttatc aacacattat gcgtactgcc gggctggact
    48421 cttcccaggg gccaacggcg gcaaaataca ttcttgagac ggtgaagccc cagcgcatcg
    48481 ccatcattca cgacaaacaa cagtatggcg aagggctggc gcgttcggtg caggacgggc
    48541 tgaaagcggc taacgccaac gtcgtcttct tcgacggtat taccgccggg gagaaagatt
    48601 tctccgcgct gatcgcccgc ctgaaaaaag aaaacatcga cttcgtttac tacggcggtt
    48661 actacccgga aatggggcag atgctgcgcc aggcccgttc cgttggcctg aaaactcagt
    48721 ttatggggcc ggaaggtgtg ggtaacgcat cattgtcgaa tattgctggc gatgctgccg
    48781 aaggcatgtt ggtcactatg ccaaaacgct atgaccagga tccggcaaac cagggcatcg
    48841 ttgatgcgct gaaagcagac aagaaagatc cgtccgggcc ttatgtctgg atcacctacg
    48901 cggcggtgca atctctggcg actgcccttg agcgtactgg cagcgatgag ccgctggcgc
    48961 tggtgaaaga tttaaaagct aacggtgcaa acaccgtgat tgggccgctg aactgggatg
    49021 aaaaaggcga tcttaaggga tttgattttg gtgtcttcca gtggcacgcc gatggttcat
    49081 ccacggcagc caagtgatca tcccaccgcc cgtgaaatgc gggcgggttt agaaaggtta
    49141 ccttatgtct gagcagtttt tatatttctt gcagcagatg tttaacggcg tcacgctggg
    49201 cagtacctac gcgctgatag ccatcggcta caccatggtt tacggcatta tcggcatgat
    49261 caacttcgcc cacggcgagg tttacatgat tggtagctat gtctccttta tgatcatcgc
    49321 cgcactgatg atgatgggca ttgataccgg ctggctgctg gtggcggcgg gattcgtcgg
    49381 cgcaatcgtc attgccagcg cctacggctg gagtatcgaa cgggtagcct atcgcccggt
    49441 gcgtaactct aagcgcctga ttgcgctcat ctcggcaatc ggcatgtcca tcttcctgca
    49501 aaactacgtt agcctgaccg aaggttcgcg tgacgtggcg ctgcccagtc tgtttaacgg
    49561 tcagtgggtg gtggggcata gcgaaaactt ctctgcctct attaccacca tgcaggcagt
    49621 gatctggatt gttactttcc tcgccatgct ggcgctgacg attttcattc gttattcccg
    49681 tatggggcgc gcctgccgtg cctgcgcgga agatctgaaa atggcgagcc tgcttggcat
    49741 caacaccgac cgggtgattg ctttgacctt tgttattggc gcggcgatgg cggcagtggc
    49801 tggcgtactg ctcggtcagt tctacggcgt cattaacccc tatatcggct ttatggccgg
    49861 gatgaaagcc tttaccgctg cggtgctcgg tgggattggc agcattccag gggcgatgat
    49921 tggcgggctg atcctgggga ttgcggaggc gctctcttct gcctatctga gtacggaata
    49981 taaagatgtg gtctcattcg ccctgctgat tctggtgctg ctggtgatgc cgaccggtat
    50041 tctgggtcgc ccggaggtag agaaagtatg aaaccgatgc atattgcaat ggcactgctc
    50101 tctgccgcga tgttctttgt cctcgctggc gtctttatgg gcgtgcaact ggagctggat
    50161 ggcaccaaac tggtggtcga cagcgcttcg gatgtccgtt ggcagtgggt gtttatcggc
    50221 acggcggtgg tctttttctt ccagcttttg cgacctgctt tccagaaagg gctaaaaagc
    50281 gtttccgggc cgaagtttat tctgcccgcg attgatggct ccacggtgaa gcagaaactg
    50341 ttcctcgtgg cgctgctggt gcttgcggtg tcgtggccgt ttatggtgtc acgtgggacg
    50401 gtggatatcg ccactctgac catgatctac attatcctcg gtctcgggct gaatgtggtt
    50461 gttggtcttt ctggtctgct ggtgctgggg tacggcggtt tttacgccat cggcgcttac
    50521 acttttgcgc tgctcaatca ctattacggt ctggggttct ggacctgttt gccgattgcc
    50581 ggactgatgg cagcggcggc gggcttcctg cttggttttc cggtgttgcg tctgcgcggt
    50641 gattatctgg cgatcgttac cctcggtttc ggcgaaattg tgcgcatatt gctgctcaat
    50701 aacaccgaaa ttaccggcgg cccgaacggt atcagtcaga tcccgaaacc gactttcttc
    50761 ggccttgagt tcagccgtac cgcccgtgaa ggcggttggg acacgttcag taatttcttt
    50821 ggcctgaaat acgatccctc cgatcgcgtc atcttcctgt atctggtggc gttgctgctg
    50881 gtggtgctaa gcctgtttgt cattaaccgc ctgctgcgga tgccgctggg gcgtgcgtgg
    50941 gaagcgttgc gtgaagatga gatcgcctgc cgttcgctgg gcttaagccc gcgtcgtatc
    51001 aagctgactg cctttaccat cagtgccgcg tttgccggtt ttgccggaac gctgtttgct
    51061 gcgcgccagg gctttgtcag cccggaatcc ttcacctttg ctgaatcggc gtttgtactg
    51121 gcgatagtgg tgctcggcgg tatgggatcg cagtttgcgg tgattctggc ggcaattttg
    51181 ctggtggtat cgcgcgaatt gatgcgtgat ttcaacgaat acagcatgtt aatgctcggt
    51241 ggtttgatgg tgctgatgat gatctggcgt ccgcagggct tgctgcccat gacgcgcccg
    51301 caactgaagc tgaaaaacgg cgcagcgaaa ggagagcagg catgagtcag ccattattat
    51361 ctgtgaacgg cctgatgatg cgcttcggcg gcctgctggc ggtgaacaac gtcaatcttg
    51421 aactgtaccc gcaggagatc gtctcgttaa tcggccctaa cggtgccgga aaaaccacgg
    51481 tttttaactg tctgaccgga ttctacaaac ccaccggcgg caccatttta ctgcgcaatc
    51541 agcacctgga aggtttaccg gggcagcaaa ttgcccgcat gggcgtggtg cgcaccttcc
    51601 agcatgtgcg tctgttccgt gaaatgacgg taattgaaaa cctgctggtg gcgcagcatc
    51661 aacaactgaa aaccgggctg ttctctggcc tgttgaaaac gccttctttc cgtcgcgccc
    51721 agagcgaagc gctcgaccgc gccgccacct ggcttgagcg cattggtttg ctggaacacg
    51781 ccaaccgtca ggcgagtaac ctggcctatg gtgaccaacg ccgtctggag attgcccgct
    51841 gcatggtgac gcagccggag attttaatgc tcgacgaacc ggcggcaggt cttaacccaa
    51901 aagaaaccaa agagctggat gagctgattg ccgaactgcg caatcatcac aacaccacta
    51961 tcttgttgat tgaacacgat atgaagctgg tgatggggat ttcagaccga atttatgtgg
    52021 tcaatcaggg gacgccactg gcaaacggta cgccggagca aatccgtaat aacccggacg
    52081 tgatccgtgc ctatttaggt gaggcataag atggaaaaag tcatgttgtc ctttgacaaa
    52141 gtcagcgccc actacggcaa aatccaggcg ctgcatgagg tgagcctgca tatcaatcag
    52201 ggcgagattg tcaccctcat tggcgcgaac ggggcgggga aaaccacctt gctcggcacg
    52261 ttatgcggcg atccgcgcgc taccagcggg cgaattgtgt ttgatgataa agacattacc
    52321 gactggcaga cggcgaaaat catgcgcgaa gcggtagcga ttgtcccgga agggcgtcgc
    52381 gtcttctcgc ggatgacggt ggaagagaac ctggcgatgg gcggcttttt tgccgaacgc
    52441 gaccagttcc aggagcgcat aaagtgggtg tatgaactgt tcccacgtct gcatgagcgc
    52501 cgtgttcagc gggcaggcac catgtccggc ggtgagcaac aaatgctggc gattggtcgc
    52561 gcgctgatga gcaacccgcg tttgctgcta cttgatgagc catcgcttgg tcttgcgccg
    52621 attatcatcc agcaaatttt cgacaccatc gagcagctgc gcgagcaggg gatgactatc
    52681 ttcctcgtcg agcagaacgc caaccaggcg ctaaagctgg cggatcgcgg ctacgtgctg
    52741 gaaaacggcc atgtggtgct ctccgatact ggtgatgcac tactggcgaa tgaagcggtg
    52801 agaagtgcgt atttaggcgg gtaataacac gttgattgat aaggagtcaa aagactcctt
    52861 tgagacaggt gacaaatgta aaattgcctg atgcgctgcg cttatcaggc ctactgggtg
    52921 agttgcaata tattgaattt gcacgatctt gtaggccgga taaggcgttt acgccgcatc
    52981 cggcatgaaa cgatgagtaa tctgtagagt ttgattcaga ccttctattt ttccgcttat
    53041 ccgtgcccca tctcccattt tccctcaccc acgccgtcac cgccttgtca tctttctgac
    53101 accttacttg caggtttatg aagttggcac cgccaccatg atggcatcga aagccattaa
    53161 accggtatat gacgtgttta aagaggcagg gattcaattc gatgagtcgc agtttgtgcc
    53221 gacggtttcc ggttactact ccgacagcaa aacgggccac ttactctccc agccattcaa
    53281 cagctcgact cccgttctct attacaacaa agacgccttc aagaaagcgg ggttagaccc
    53341 ggaacagccg ccgaaaacct ggcaggatct ggcggactct tccgcaaaac tgaaagcctc
    53401 cggtatgaag tgcggctacg ccagcggctg gcagggctgg atccaactgg aaaactttag
    53461 cgcctggaac ggtctgccgt ttgccagcaa aaacaacggc tttgacggca cggacgcggt
    53521 gctggagttc aacaagccgg agcaggtgaa acacattgcc atgctcgaag agatgaacaa
    53581 gaagggcgac ttcagctacg tcggtcgtaa ggatgaatcc accgagaagt tctataacgg
    53641 tgattgcgcg atgaccaccg cctcttccgg ttctctcgct aatattcgtg agtacgccaa
    53701 atttaactat ggcgtaggca tgatgcctta cgacgccgat gcgaaagatg caccgcaaaa
    53761 cgccattatc ggcggggcca gcctgtgggt gatgcagggc aaaaataaag aaacgtatac
    53821 cggcgtggcg aagttcctcg acttcctcgc gaagccagaa aacgctgccg agtggcatca
    53881 gaaaaccggt tatctgccaa tcaccaaagc agcgtatgac ctgacccgtg agcagggctt
    53941 ttatgagaaa aacccagggg cggataccgc gacgcgtcag atgctgaata agccgccgtt
    54001 gccgttcacc aaagggctgc gtctgggcaa catgccgcag atccgcgtga ttgtggatga
    54061 agagctggag agcgtgtgga ccggtaagaa gacaccacag caggcactgg ataccgccgt
    54121 tgagcgtgga aatcagttgc tgcgccgctt tgagaaatcg acgaagtctt aatcagtgta
    54181 atgtcggatg cgtttcgctt atctgacctg gcatcgcgtg taggccggat aagcgaagcg
    54241 catccggcac agttcaggaa ttaaccgtaa tgtcatcatc ccgtccggtg ttccgctcgc
    54301 gctggctgcc ttatctgctg gtcgcgccgc agctcatcat caccgttatc ttttttatct
    54361 ggcctgcggg cgaagcgttg tggtactcgc ggcaaagcgt cgatccgttt ggtttctcca
    54421 gccagtttgt cggtctggat aacttcgtca cgctgttcca tgacagctac tatctagact
    54481 ccttctggac gacgataaaa ttcagcacct ttgtcaccgt cagcggtttg ctggtatcgc
    54541 tgttctttgc ggcgctggtg gagtacatcg tgcgcggcag ccgtttctat caaaccttga
    54601 tgctgctgcc gtatgccgtg gctcccgccg ttgccgccgt attgtggatc ttcctgttta
    54661 accccggtcg cgggctgatc acccattttc tcgctgagtt cggctacgac tggaaccacg
    54721 cgcaaaacag cggtcaggca atgtttctgg tggtgtttgc ctcggtgtgg aagcaaatca
    54781 gctacaactt tctgttcttc tatgccgcgc tgcactccat tccccgttcg ttgattgaag
    54841 ccgcagctat tgacggcgca gggccgattc gccgcttctt taagattgct ctgccgctta
    54901 tcgctccggt gagtttcttc ctgctggtgg tgaacctggt gtacgccttc ttcgacacct
    54961 tcccggtgat cgacgccgcc acgtccggcg ggccggttca ggccaccact acgctgattt
    55021 ataagatcta ccgcgaaggc tttaccggac tggatctggc ttcgtctgcc gcgcagtcgg
    55081 tggtgttgat gttcctcgtc atcgtgctga cggtggtgca gttccgctat gttgaaagca
    55141 aggtgcgtta ccaatgattg agaaccgtcc gtggctgacg atattcagcc ataccatgct
    55201 gatcctcggg atcgcggtga tcctcttccc gctgtacgtg gcgtttgtcg cggcgacgct
    55261 ggataaacag gccgtctatg ccgcgccgat gacgctcatc cccggcacac atctgctgga
    55321 aaatatccac aacatctggg ttaacggggt aggcacgaat agcgcgccgt tctggcggat
    55381 gttgcttaac agctttgtga tggcgttcag cattacgctc ggcaaaatta ccgtctcgat
    55441 gctctcggca tttgccattg tctggtttcg ttttccgcta cgtaacctct tcttctggat
    55501 gatttttatc accctgatgc tgccggttga agtacgtatc ttcccgacgg tggaagtcat
    55561 cgccaacctg aaaatgctcg acagctacgc cggtttaacg ctgccgctga tggcctcggc
    55621 gaccgccacg tttctgttcc gccagttctt tatgacccta ccggatgaat tggtggaagc
    55681 cgcgcggatc gacggcgcgt caccaatgcg tttcttttgc gacatcgttt ttccgttgtc
    55741 caaaactaat ctggcggcgc tgtttgtgat cacctttatc tacggctgga atcagtattt
    55801 gtggccgttg ttgattatta ccgacgttga tctcggcacc accgtggcag ggatcaaagg
    55861 gatgatcgct acaggcgaag gcaccacgga atggaactcg gtaatggcgg cgatgttgtt
    55921 aacgcttatc cctccggtgg tgattgtttt agtgatgcag cgtgccttcg tgcgcggcct
    55981 ggtcgatagt gagaaataag atggcaggac tgaaattaca ggcagtaacc aaaagctggg
    56041 atggcaaaac ccaggtcatt aaaccgctga cccttgatgt ggcggatggc gaatttatcg
    56101 tgatggtcgg gccgtctggc tgcgggaaat cgacgctgct gcgcatggtt gccgggctgg
    56161 agcgggtgac agaaggcgat atctggatca acgaccagcg cgtgaccgaa atggagccga
    56221 aagatcgcgg gattgcgatg gtgttccaga attacgcgct ttatccgcat atgagcgtag
    56281 aggagaacat ggcgtggggg ctgaaaattc gcggcatggg caagcagcaa attgccgagc
    56341 gcgttaaaga agcggcgcgc attctggaac tggacggtct gcttaagcgt cgtccgcgtg
    56401 agctatccgg cggtcagcgc cagcgtgtgg cgatgggccg cgcgattgtg cgcgatccgg
    56461 cggtgttctt gtttgatgag ccgctctcta acctcgatgc caagctgcgc gtgcagatgc
    56521 gtcttgaact gcaacagttg caccgtcgcc tgaaaacgac ttcactctac gttactcacg
    56581 atcaggttga agcgatgacg ctcgcccagc gagtaatggt gatgaacggt ggcgttgccg
    56641 aacagattgg cacaccagtt gaagtctacg aaaagcccgc cagcctgttt gtggcgagtt
    56701 ttatcggcag cccggcgatg aacctgctga caggccgcgt gaataatgaa ggcacgcatt
    56761 tcgaactgga cggcggtatc gtgctaccgc taaacggtgg ctaccgtcag tatgccgggc
    56821 gtaaaatgac tctcggcatt cgcccggaac atatagcgct aagctcgcag gcagaaggtg
    56881 gtgtgccgct ggtgatggac acgctggaga tcctcggcgc agataacctg gcgcacggac
    56941 gctggggcga gcagaagctg gtggtgcgac tggcgcatca ggagcgcccg acggcaggca
    57001 gcacgctgtg gctgcatttg gcggaaaatc agctgcatct ttttgatggt gaaacaggac
    57061 aacgagtatg agtaactggc cttatccccg catcgtcgct catcgtggcg gcggtaagct
    57121 ggccccggaa aacaccctgg cggcaatcga cgtcggggca aaatacggtc ataagatgat
    57181 cgaatttgac gcgaagttat cgaaagatgg cgagatcttc ctgctccatg acgacaatct
    57241 cgaacgcacc agcaacggct ggggcgtcgc gggtgaactg aactggcagg atttactgcg
    57301 cgtggatgcg ggcagttggt acagcaaagc gtttaaaggt gagccgctgc cgttgctttc
    57361 gcaggtggcg gaacgctgtc gcgaacacgg gatgatggcg aatatcgaaa tcaaacccac
    57421 cactggcact ggaccattaa cgggcaaaat ggtggcgctg gcggcacgcc aactgtgggc
    57481 cggtatgacg ccgccgctgc tgtcatcgtt tgagattgat gctttagaag ctgcacaaca
    57541 ggcggcaccg gaactgccgc gtggtttatt gcttgatgag tggcgcgacg actggcgcga
    57601 actgaccgcg cggctgggct gcgtctctat tcatctcaat cataagttac tcgataaagc
    57661 gcgagtgatg cagttgaaag acgccggatt acggattctg gtttataccg tcaataaacc
    57721 ccagcgcgcg gcagagttgc tgcgctgggg cgtggattgc atctgtaccg atgcgattga
    57781 cgtgattggt ccgaacttta cggcccaata gttttcaacg gaatgtcagg ctgcggcgtg
    57841 ctgggctgat tcagcatgtc gccgtttctc ctttccggca gcatatgctg ctgattcaac
    57901 gaactatccg gattacggtt gctgtttaac atcccgccgt tggtgttggg cagcatttgc
    57961 tgccgggcgg gatttcgctc ccccggctgt gactgcaaca cccgctgaga attattgttt
    58021 atctggtttt ctaaatgctg ctgttgcaac tgagtttgcg ttttcagttg ctgattcagc
    58081 atcccttttt gctggatttg ctgagtctgc atctgggttt gcatccgctg ctggctgggt
    58141 atctgatacc ccggctggtt agggttgttc agagtattaa tgggctgtgc aaagccgaca
    58201 aacggcagga gtgccgtaag aatcagaagt cgtttcatcg cgtatcctcc tctgaagata
    58261 tcctttaagt ttactcgctt cccgacaaaa cgatgattaa ttcagagtta tataccaggc
    58321 ttagctgggg ttgcccctta atctctggag aataacgatg ataaaaccga cgtttttacg
    58381 ccgggtggcc attgctgctc tgctctcagg aagttgtttt agcgccgccg ccgcgcctcc
    58441 tgcgccgccc gtctcgtatg gtgtggagga agatgtcttc cacccggtac gcgcgaaaca
    58501 gggaatggta gcgtctgtgg acgccactgc cactcaggtg ggggtggata tcctcaagga
    58561 gggcgggaat gccgttgatg ccgccgtggc ggtgggctac gcgctggcgg taacgcatcc
    58621 gcaggcaggg aatctgggcg gtggtggttt tatgttaatc cgctcgaaaa atggcaatac
    58681 cacggctatc gatttccgcg aaatggcacc cgccaaagcg acccgcgata tgttcctcga
    58741 tgatcagggc aacccggaca gcaaaaaatc actcacttcg catctggctt ccggcacacc
    58801 gggtacggta gcaggtttct cgctggcgct ggataaatac ggcaccatgc cgctgaacaa
    58861 agtcgtgcag cccgcgttta aactggcacg cgatggtttt atcgttaacg acgcgctggc
    58921 tgacgatctc aaaacctacg gtagcgaagt gttgccgaat cacgaaaaca gtaaagctat
    58981 cttctggaaa gagggcgagc cgctgaaaaa gggcgacacg ctggtgcagg cgaacctggc
    59041 aaagagcctg gagatgattg ctgaaaacgg cccggacgaa ttctataaag gcacgattgc
    59101 ggaacagatc gcccaggaga tgcagaaaaa cggtggcttg atcactaaag aagatttagc
    59161 ggcctataaa gcggtcgaac gcactccgat aagcggcgat tatcgcgg
//
LOCUS       DAJVRF010000007        58571 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJVRF010000007 DAJVRF010000000
VERSION     DAJVRF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58571
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.007"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..153
                     /locus_tag="OQN13_000392"
     CDS             <1..153
                     /locus_tag="OQN13_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCS1613856.1"
                     /translation="LRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTF
                     YTPQGS"
     gene            182..346
                     /locus_tag="OQN13_000393"
                     /pseudo
     CDS             182..346
                     /locus_tag="OQN13_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244718.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            complement(384..1445)
                     /locus_tag="OQN13_000394"
     CDS             complement(384..1445)
                     /locus_tag="OQN13_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCS1613857.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1658..3121
                     /gene="bepA"
                     /locus_tag="OQN13_000395"
     CDS             1658..3121
                     /gene="bepA"
                     /locus_tag="OQN13_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCS1613858.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            3142..3501
                     /gene="arsC"
                     /locus_tag="OQN13_000396"
     CDS             3142..3501
                     /gene="arsC"
                     /locus_tag="OQN13_000396"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCS1613859.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3639..4385)
                     /gene="hda"
                     /locus_tag="OQN13_000397"
     CDS             complement(3639..4385)
                     /gene="hda"
                     /locus_tag="OQN13_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCS1613860.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(4435..5724)
                     /gene="uraA"
                     /locus_tag="OQN13_000398"
     CDS             complement(4435..5724)
                     /gene="uraA"
                     /locus_tag="OQN13_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCS1613861.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5810..6436)
                     /gene="upp"
                     /locus_tag="OQN13_000399"
     CDS             complement(5810..6436)
                     /gene="upp"
                     /locus_tag="OQN13_000399"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCS1613862.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6761..7798
                     /gene="purM"
                     /locus_tag="OQN13_000400"
     CDS             6761..7798
                     /gene="purM"
                     /locus_tag="OQN13_000400"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCS1613863.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7798..8436
                     /gene="purN"
                     /locus_tag="OQN13_000401"
     CDS             7798..8436
                     /gene="purN"
                     /locus_tag="OQN13_000401"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCS1613864.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8607..10673
                     /gene="ppk1"
                     /locus_tag="OQN13_000402"
     CDS             8607..10673
                     /gene="ppk1"
                     /locus_tag="OQN13_000402"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCS1613865.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYVASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10678..12219
                     /gene="ppx"
                     /locus_tag="OQN13_000403"
     CDS             10678..12219
                     /gene="ppx"
                     /locus_tag="OQN13_000403"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCS1613866.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12258..14501)
                     /gene="pdeF"
                     /locus_tag="OQN13_000404"
     CDS             complement(12258..14501)
                     /gene="pdeF"
                     /locus_tag="OQN13_000404"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCS1613867.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14683..14835)
                     /locus_tag="OQN13_000405"
     CDS             complement(14683..14835)
                     /locus_tag="OQN13_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1613868.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14853..15044
                     /locus_tag="OQN13_000406"
     CDS             14853..15044
                     /locus_tag="OQN13_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCS1613869.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            15355..15873
                     /locus_tag="OQN13_000407"
     CDS             15355..15873
                     /locus_tag="OQN13_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCS1613870.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            15889..16428
                     /locus_tag="OQN13_000408"
     CDS             15889..16428
                     /locus_tag="OQN13_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCS1613871.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16523..18100)
                     /gene="guaA"
                     /locus_tag="OQN13_000409"
     CDS             complement(16523..18100)
                     /gene="guaA"
                     /locus_tag="OQN13_000409"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCS1613872.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(18169..19635)
                     /gene="guaB"
                     /locus_tag="OQN13_000410"
     CDS             complement(18169..19635)
                     /gene="guaB"
                     /locus_tag="OQN13_000410"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCS1613873.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            19797..21167
                     /gene="xseA"
                     /locus_tag="OQN13_000411"
     CDS             19797..21167
                     /gene="xseA"
                     /locus_tag="OQN13_000411"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCS1613874.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(21164..21379)
                     /locus_tag="OQN13_000412"
     CDS             complement(21164..21379)
                     /locus_tag="OQN13_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCS1613875.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(21448..22920)
                     /gene="der"
                     /locus_tag="OQN13_000413"
     CDS             complement(21448..22920)
                     /gene="der"
                     /locus_tag="OQN13_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCS1613876.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(23038..24216)
                     /gene="bamB"
                     /locus_tag="OQN13_000414"
     CDS             complement(23038..24216)
                     /gene="bamB"
                     /locus_tag="OQN13_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCS1613877.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(24227..24847)
                     /gene="yfgM"
                     /locus_tag="OQN13_000415"
     CDS             complement(24227..24847)
                     /gene="yfgM"
                     /locus_tag="OQN13_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCS1613878.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(24865..26139)
                     /gene="hisS"
                     /locus_tag="OQN13_000416"
     CDS             complement(24865..26139)
                     /gene="hisS"
                     /locus_tag="OQN13_000416"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCS1613879.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(26250..27368)
                     /gene="ispG"
                     /locus_tag="OQN13_000417"
                     /gene_synonym="gcpE"
     CDS             complement(26250..27368)
                     /gene="ispG"
                     /locus_tag="OQN13_000417"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCS1613880.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(27395..28408)
                     /gene="rodZ"
                     /locus_tag="OQN13_000418"
     CDS             complement(27395..28408)
                     /gene="rodZ"
                     /locus_tag="OQN13_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCS1613881.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(28693..29847)
                     /gene="trmG/rlmN"
                     /locus_tag="OQN13_000419"
     CDS             complement(28693..29847)
                     /gene="trmG/rlmN"
                     /locus_tag="OQN13_000419"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCS1613882.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(29997..30428)
                     /gene="ndk"
                     /locus_tag="OQN13_000420"
     CDS             complement(29997..30428)
                     /gene="ndk"
                     /locus_tag="OQN13_000420"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCS1613883.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(30577..32889)
                     /gene="pbpC"
                     /locus_tag="OQN13_000421"
     CDS             complement(30577..32889)
                     /gene="pbpC"
                     /locus_tag="OQN13_000421"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCS1613884.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(32890..37851)
                     /gene="yfhM"
                     /locus_tag="OQN13_000422"
     CDS             complement(32890..37851)
                     /gene="yfhM"
                     /locus_tag="OQN13_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCS1613885.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNCLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            38058..38903
                     /gene="sseA"
                     /locus_tag="OQN13_000423"
     CDS             38058..38903
                     /gene="sseA"
                     /locus_tag="OQN13_000423"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCS1613886.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(39396..40172)
                     /gene="sseB"
                     /locus_tag="OQN13_000424"
     CDS             complement(39396..40172)
                     /gene="sseB"
                     /locus_tag="OQN13_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCS1613887.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(40314..41597)
                     /gene="pepB"
                     /locus_tag="OQN13_000425"
     CDS             complement(40314..41597)
                     /gene="pepB"
                     /locus_tag="OQN13_000425"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCS1613888.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(41775..41975)
                     /gene="iscX"
                     /locus_tag="OQN13_000426"
     CDS             complement(41775..41975)
                     /gene="iscX"
                     /locus_tag="OQN13_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCS1613889.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(41987..42322)
                     /gene="fdx"
                     /locus_tag="OQN13_000427"
     CDS             complement(41987..42322)
                     /gene="fdx"
                     /locus_tag="OQN13_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCS1613890.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(42324..44174)
                     /gene="hscA"
                     /locus_tag="OQN13_000428"
     CDS             complement(42324..44174)
                     /gene="hscA"
                     /locus_tag="OQN13_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCS1613891.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(44191..44706)
                     /gene="hscB"
                     /locus_tag="OQN13_000429"
     CDS             complement(44191..44706)
                     /gene="hscB"
                     /locus_tag="OQN13_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCS1613892.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(44802..45125)
                     /gene="iscA"
                     /locus_tag="OQN13_000430"
     CDS             complement(44802..45125)
                     /gene="iscA"
                     /locus_tag="OQN13_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCS1613893.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(45142..45528)
                     /gene="iscU"
                     /locus_tag="OQN13_000431"
     CDS             complement(45142..45528)
                     /gene="iscU"
                     /locus_tag="OQN13_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCS1613894.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(45556..46770)
                     /gene="iscS"
                     /locus_tag="OQN13_000432"
     CDS             complement(45556..46770)
                     /gene="iscS"
                     /locus_tag="OQN13_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCS1613895.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(47011..47499)
                     /gene="iscR"
                     /locus_tag="OQN13_000433"
     CDS             complement(47011..47499)
                     /gene="iscR"
                     /locus_tag="OQN13_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCS1613896.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(47800..48537)
                     /gene="trmJ"
                     /locus_tag="OQN13_000434"
     CDS             complement(47800..48537)
                     /gene="trmJ"
                     /locus_tag="OQN13_000434"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCS1613897.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            48656..49459
                     /gene="suhB"
                     /locus_tag="OQN13_000435"
     CDS             48656..49459
                     /gene="suhB"
                     /locus_tag="OQN13_000435"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCS1613898.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            49604..50458
                     /locus_tag="OQN13_000436"
     CDS             49604..50458
                     /locus_tag="OQN13_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1613899.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            50649..51929
                     /gene="csiE"
                     /locus_tag="OQN13_000437"
     CDS             50649..51929
                     /gene="csiE"
                     /locus_tag="OQN13_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCS1613900.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(51921..53060)
                     /locus_tag="OQN13_000438"
     CDS             complement(51921..53060)
                     /locus_tag="OQN13_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCS1613901.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(53220..54110)
                     /gene="hcaR"
                     /locus_tag="OQN13_000439"
     CDS             complement(53220..54110)
                     /gene="hcaR"
                     /locus_tag="OQN13_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCS1613902.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            54246..55607
                     /locus_tag="OQN13_000440"
     CDS             54246..55607
                     /locus_tag="OQN13_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCS1613903.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            55604..56122
                     /locus_tag="OQN13_000441"
     CDS             55604..56122
                     /locus_tag="OQN13_000441"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCS1613904.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            56122..56442
                     /locus_tag="OQN13_000442"
     CDS             56122..56442
                     /locus_tag="OQN13_000442"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCS1613905.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            56439..57251
                     /gene="hcaB"
                     /locus_tag="OQN13_000443"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             56439..57251
                     /gene="hcaB"
                     /locus_tag="OQN13_000443"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCS1613906.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            57261..58463
                     /locus_tag="OQN13_000444"
     CDS             57261..58463
                     /locus_tag="OQN13_000444"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCS1613907.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        14316 a        15841 c        14612 g        13802 t
ORIGIN      
        1 ctcagggatc cgctctccag ggcttactac acccgcaaaa tgagtcaggg aaaacgacac
       61 aatcaggcgc ttatcgccct ggcgagacga cgctgcgacg ttctgttcgc catgatgcgc
      121 gacgggactt tttatacccc gcaggggtca taacatgctt gacaacttaa taggggcacc
      181 ccccttctgg caactggcgc acagtagcgc agacaatttt ccggcactga cggtaagcca
      241 ttttattacc gccaatctgc tcccggtgat gctgggtaat attatcggcg gtgcagtgct
      301 ggtgagtatg tgttatcggg ctatttattt acgtcaggaa tcctgaaaaa tcagcccggc
      361 gaaacagttc gtcgggctga agattattct tgcgctattt gcccatcggg ccaggcgtgg
      421 atcacggctt tgatcagcgt cgccaatgga atagcgaaga atacgcccca gaatccccac
      481 aaaccaccga agatcaccac cgataaaata atcaccagcg gatgcaggtt aaccgcttcg
      541 gagaacaaca ccggtactaa cagattaccg tccagcgcct gaataatcag atacactgcg
      601 aagcagctcc agaattccgt gcctgcacca aactggaata gcgccacgcc aaccaccgga
      661 atggtcacca caaatgcgcc aatgtacgga ataagtaccg agaagccgac cagcaccgcc
      721 agcagcagcg aatagttcag cccaaagagc aagaacccca gccaagtggc gatccccacc
      781 acgatcatct ccagcacttt gccgcggata tagttggtga tttgttgatt catctccttc
      841 cacacctgtc ctgccagtcc acggttgcgc ggcagcaccc gacgaacggc gttcagcatc
      901 tgctctttgt ctttcagcag gaagaagacc atcaatggta ccagcaccag atagacggca
      961 atggtcagca aaccgaccag cgaggcgagg gaaattttca ccaccgaatc gcccatggtc
     1021 agcatccgac tgcgcatatt ttcggccatt gcatcaataa tgcccgcgtc cattaatgcc
     1081 ggatagcggc gcggcaacgt ggcggcaaag tcagaaagct tattgagcat ccccggcata
     1141 tcgcggatca agtagatgcc ctgttgccag gcgataggca gtaccacgaa cgccatcagt
     1201 agcaatatac cgacgaaaac caccaataca atcgacgtcg cccagcggcg ggagcagcca
     1261 atagattgca ggcgcacggt tggccattcc agcaaatagg ccagcacaat agccaccagc
     1321 aacggagcaa gcaggccact aaagaaaaag ataatgccaa atccggcaac taaaataacc
     1381 agcaaggcaa tcgcttccgg gtcgctaaaa cggcggcgat accattgcat caacatttcg
     1441 agcataaaga ccttccctga acctcaagag cgggattgcg atccgcaatt gtatcgaaat
     1501 gtcacaaaaa agacttcgct ttttatgacg gattcaggaa actgaaaagt catttgagtg
     1561 ggctaatctt cgccgttaca ctcaaaggcg gcgcggtggg aacaatattt cacagtatcg
     1621 gtcaaatgac tatcttcaga aatacaggat agaggttatg ttcaggcagt tgaaaaaaaa
     1681 cctggttgca accctcattg ctgctatgac cattggtcag gtagccccgg cgtttgccga
     1741 cagcgcagac accttgccgg atatgggaac ctccgcagga agcacgcttt ccattggtca
     1801 ggaaatgcag atgggcgact attatgtccg ccagctacgc ggcagcgcgc cgttaattaa
     1861 tgacccgctg ttaacgcaat atattaattc gctggggatg cgtctggttt cgcatgccaa
     1921 ttcggttaag acaccgtttc atttttttct gatcaacaac gacgaaatta acgcctttgc
     1981 tttctttggc ggcaacgtgg tgctgcactc tgccctgttc cgttattccg ataacgaaag
     2041 tcaactggct tcagttatgg cgcacgaaat ctcccacgtc acccaacgtc acctggcgcg
     2101 agcgatggaa gatcagcagc gcagcgcgcc gctgacctgg gtcggcgcgt taggttctat
     2161 tttactggcg atggccagtc cgcaggcggg gatggcggcg ctgaccggta cactggcggg
     2221 aacgcgtcag gggatgatca gtttcaccca gcaaaatgaa caggaagcgg accgcattgg
     2281 tattcaggtg ctgcaacgct cgggattcga tccgcaggcg atgccaacct tcctcgaaaa
     2341 attactcgat caggcgcgtt actcctcgcg cccgccggaa attttattga ctcacccgtt
     2401 gccggaaagt cgtctggcag atgcccgcaa ccgtgctaat cagatgcgcc cgatggtggt
     2461 gcagtcgtcg gaagatttct atctggcgaa agcgcgcaca ctggggatgt ataattccgg
     2521 acgtaaccag ctcaccagtg atttgctgga tgaatgggcg aaaggaaacg ttcgtcagca
     2581 acgagcggcg caatatggtc gtgctttaca ggcgatggaa gccaataaat acgacgaggc
     2641 gcgtaaaacg ctgcaaccgt tactggcggc agaacctggc aacgcatggt atctcgatct
     2701 ggctaccgat atcgatcttg ggcaaaacaa agccaatgag gcaatcaatc gcctgaaaaa
     2761 tgcccgtgat ttgcgcacca atccggtgtt gcagctcaac ctggcaaacg cttatctgca
     2821 aggcggtcaa ccacaagaag cggccaatat tctgaatcgc tacaccttta ataataaaga
     2881 tgacagcaac ggctgggatt tactggcaca ggcggaagcc gcgctaaata accgcgatca
     2941 ggagctggct gcgcgagcag aaggttatgc gctcgccgga cgactcgatc aggccatttc
     3001 gctgttgagt agcgccagtt cgcaggtgaa attaggcagc cagcaacaag cgcgttacga
     3061 tgcgcgcatc gaccagttgc gccagctgca ggaacgcttt aagccttata ccaagatgta
     3121 attcgcatta aaggaagaac aatgaccaaa caggtaaaaa tctaccataa cccacgctgt
     3181 tcaaagagcc gggaaacgct gaacctgctg aaaaaaaacg gcgtggagcc agaagtggta
     3241 ctctaccttg agacacccgc cgatgcggca acgctgcgcg atttgctgaa aatgctgggg
     3301 atgaatagcg cccgtgaatt gatgcgccag aaagaggatc tttataaaga actgaacctg
     3361 gctgacagtt cgcttagcga agaggcgctg attcaggcaa tggtggataa tccgaagctg
     3421 atggagcgcc cgattgtggt cgcgaatggc aaagcgcgga ttggtcgtcc accggagcag
     3481 gtactggaaa tcgtgggtta agatgtgccc catcgctagt tgaagcacat tgccggatgc
     3541 gacgcttgcc atgtcttatc cggcctacga atactatgga ttaacttcgc gtagttcgga
     3601 taaggcgttc gcgccgcatc cgacaataaa caccttatct acaacttcag aatttctttc
     3661 acaaacggaa tggtcagctt acgttgcgcg gtaatcgacg cacgatccaa ctgatccaac
     3721 gtcataaata gcgtgcgcat ttctctgtcg agccgcttca gcaagaaacg ccccacatct
     3781 tccggcagtt caaaaccacg caaacgcgcg cgtaactgta gcgcctgcaa cttatcttca
     3841 tcagaaagtg gctgcaattt gtagatctgc ccccagtcga gtcgcgacgc gagatccggt
     3901 aatcccagat tcaactgccg cggtggacga tcgccggtga tcaacaatcg tgttttgccc
     3961 gattccagaa ttcgattgta gagatcgaaa atcgccatct cccacaactc atcgcctgca
     4021 atacactcaa tgttgtcgat acagaccagc gacaaatgct ccataccgtc gagcacttcc
     4081 ggaacaaacc aggtgcgttt atccagcggg acatagccca ccgcatcgcc acgctgcgac
     4141 aattccgcgc aagccgcgtg cagcagatgg ctgcgccccg cgccttcgcg tgcccagaga
     4201 tagatgtaac cgctatgttc ctgacgcagc acgttttgca gcgcagccag taaagaggag
     4261 ttatcccccg gccagaaact tgcaaaggtt tcgtcgtcag gaagataaag tggcaaagag
     4321 agctgtgccg gtgtgttcag agatacctca accaggattt cacaaaatcg cgagaagttt
     4381 accacagaat cccatgatgt ttgaaccggg cagcaacact gcccggtcgg tacattattt
     4441 gtctgttata tccgcgtctt ctgcgtccag caccacttct tcagggcgca gcacactaat
     4501 cagtttgaag atcaaactta agccgatacc gacgatggtc gccagcgcca tacctttcag
     4561 ctccgccgcg ccaatgttta ccttcgcgcc actgacaccg atgatcaaaa tcacggaagt
     4621 caggatcagg ttctgtgctt tgttgtaatc cacttttgat tcgatcaaaa cacgaatacc
     4681 ggaagcaccg atgacaccat aaagcagcag cgaaacgccg cccatcaccg gcaatgggat
     4741 catctggata gcggcagcca gtttaccgac gcaggaaagc aggatagcga aaatcgccgc
     4801 cccaccgata acccaggtac tgtaaacacg ggtgatcgcc atcacgccaa tgttttctcc
     4861 gtaagtggta tttggcgtag agccaaagaa gccggagatc acagtcgaca ggccgttagc
     4921 aaacatcgaa cggtgcagac ctggatcgcg cagcagatct tttttgacga tattagccgt
     4981 tactaccagg tgccctacgt gttcggcaat aaccactaac gccgctggca gaatagtcag
     5041 aatggcaaac cactcgaagc gcggcgtata gagggttggc agcgcaaacc agtgagcatt
     5101 aataatcggc gtggtatcga caattcccat tgcgaaagag agcgcgtacc ccaccagcac
     5161 gccaattaaa atcgggataa ttgccaggaa accacggaac agcacggaac ctaaaaccgt
     5221 gaccgccagg gtggtaatag agatgatgat ggttttggag tctggcgttt gcccttcagc
     5281 cgggagtaaa cccgccatac cggcagctac gcccgccagc tccagaccga tgacggcaac
     5341 gattgcgccc attgccgcag gtggaaacag cacgtccagc cagccggtcc ccgctttctt
     5401 cacgataaaa gaaaccaggc agaacagcac gccgcacata ataaagccgc ccagcgcgac
     5461 ttcataccct aacggcaaca gtaacaatac cggtgaaata aaggcaaagc tggaaccaag
     5521 ataagccgga attttccctt tacagatgaa gagatacagc agcgttccaa taccgttaaa
     5581 taacagtaca gtcgccgggt taatatgaaa taagacgggc accaggacgg ttgcaccaaa
     5641 catggcgaac aaatgttgca aactaagcgg gattgtctgt aaaagtggcg gtctttcact
     5701 caccccgata gcacggcgcg tcatagtatt atcctctgta ttatgtgtta taggcgcttt
     5761 actcaaaaaa aagccgactc ttaaagtcgg ctttaattat ttttattctt tatttcgtac
     5821 caaagatttt gtcaccggca tcgccgaggc ccggaataat gtatccgtgc tcgttcagtc
     5881 cctggtcaat cgatgcggta tacagttcga cgtccgggtg cgctttttcc agcgcagcga
     5941 taccttctgg cgcagctacc agcaccagaa ctttgatgct gctgcagccc gcttttttca
     6001 gcaggtcgat ggtcgcgata acggaaccac cggttgccag cattgggtca acgatcagcg
     6061 ccatacgctc atcgatgtta gaaaccagtt tctggaagta cggtaccggc tccagcgttt
     6121 cttcattacg gtacataccg acaacgctga tgcgcgcgct cggaacattt tccagcacac
     6181 cgtccatcat ccccagaccg gcacgcagaa ttggcacaac ggtaattttc ttacctttga
     6241 tctggtcgat ttctaccggg ccgttccagc cttcgatagt tactttttcc gtttcgaggt
     6301 cggcggtcgc ttcgtaagtc agcaggctac ccacttcgga agcgagttcg cgaaagcgct
     6361 tggtgctgat atcttgctca cgcatcagtc ccagcttgtg tttgacgagt gggtgtttga
     6421 cttccacgat cttcatactc tttctccttt gaggggcagc cacaaaaaaa atcgacggat
     6481 tatacctcct ttcttcaagg cggcaatatt cttttcgttg actttagtca aaatgataac
     6541 ggtttgagat aaagttattt tatattcaaa tggttatgaa agaagattat tccatccgaa
     6601 aactaacctt tgccctggca caagtcttct ttcgccgcgc gtctggggaa aagacgtgca
     6661 aaaaggttgt gcaaagcagt ctcgaaaacg tttgctttcc ctgttagaat tgcgccgaat
     6721 tttatttttc taccgcaagt aacgcgtggg gacccaagca gtgaccgata aaacctctct
     6781 tagctacaaa gatgccggtg ttgatattga cgcgggtaat gctctggttg gaagaatcaa
     6841 aggcgtagtg aagaaaacgc gtcgtccgga agtgatgggc ggtctgggcg gcttcggtgc
     6901 gctgtgtgca ttgccgcaaa aatatcgtga acccgtgctg gtttccggca ctgacggcgt
     6961 aggtaccaag ctgcgtctgg caatggactt aaaacgtcac gacaccattg gtattgatct
     7021 ggtcgccatg tgcgttaatg acctggtggt gcaaggtgca gaaccgctgt ttttcctcga
     7081 ctattacgca accggaaaac tggatgttga taccgcttca gcggtgatca gcggtatcgc
     7141 ggaaggatgt ctgcaatcgg gctgttcact ggtgggtggc gaaacggcag aaatgccggg
     7201 gatgtatcac ggtgaggatt acgatgtcgc gggtttctgc gttggcgtgg tagaaaaatc
     7261 agaaatcatc gacggctcta aagtcagcga cggcgatgtg ctgattgcac tcggttccag
     7321 cggtccgcac tcgaacggct attcgctggt gcgcaaaatt cttgaagtca gcggttgtga
     7381 tccgcaaacc accgaacttg atggtaagcc attagccgat catctgctgg caccgacccg
     7441 catttacgtg aagtcagtgc tggagttgat tgaaaaggtc gatgtgcatg ccattgcgca
     7501 cctgaccggc ggcggcttct gggaaaacat tccgcgcgta ttgccagata atactcaggc
     7561 agtgattgat gaatcttcct ggcagtggcc ggaagtgttc aactggctgc aaacggctgg
     7621 taacgttgag cgccatgaaa tgtatcgcac cttcaactgc ggcgtcggga tgattatcgc
     7681 cctgcctgct ccggaagtgg acaaagccct cgccctgcta aatgccaacg gtgaaaacgc
     7741 gtggaaaatc ggtatcatca aagcctctga ttccgaacaa cgcgtggtta tcgaataatg
     7801 aatattgtgg tgcttatttc cggcaacgga agtaatttac aggcaattat tgacgcctgt
     7861 aaaaccaaca aaattaaagg caccatacgg gcagttttca gcaataaggc cgacgcgttc
     7921 ggccttgaac gcgcccgcca ggcgggtatt gcaacgcata cgctcatcgc cagcgcgttt
     7981 gacagtcgtg aagcctatga ccgggagttg attcatgaaa tcgacatgta cgcacccgat
     8041 gtggtcgtgc tggctggttt tatgcgcatt ctcagcccgg cgtttgtctc ccactatgcc
     8101 gggcgtttgc tgaacattca cccttctctg ctgccgaaat atcccggatt acacacccat
     8161 cgtcaggcgc tggaaaatgg tgatgaagag cacggtacat cggtgcattt cgtcaccgat
     8221 gaactggacg gtggcccggt tattttacag gcgaaagtcc cggtatttgc tggtgatacg
     8281 gaagatgaca tcaccgcccg cgtgcaaacc caggaacacg ctatttatcc actggtgatt
     8341 agctggtttg ccgatggtcg tctgaaaatg cacgaaaacg ccgcgtggct ggatggtcaa
     8401 cgtctgccgc cgcagggcta cgctgccgac gagtaatgcc ccgtagttaa agcgccagct
     8461 ctgccgctgg cgtttttcaa ttcacctgta aatcgcaagc tccagcagtt tttttccccc
     8521 ttttctggca tagttggaca tctgccaata ttgctcgcca taatatccag gcagtgtccc
     8581 gtgaataaaa cggagtaaaa gtggtaatgg gtcaggaaaa gctatacatc gaaaaagagc
     8641 tcagttggtt atcgttcaat gaacgcgtgc ttcaggaagc ggcggacaaa tctaacccgc
     8701 tgattgaaag gatgcgtttc ctggggatct attccaataa ccttgatgag ttctataaag
     8761 tccgcttcgc tgaactgaag cgacgcatca ttattagcga agaacaaggc tccaactctc
     8821 attcccgcca tttactgggc aaaattcagt cccgggtgct gaaagccgat caggaattcg
     8881 acggcctcta caacgagctg ctgctggaga tggcgcgtaa ccagatcttc ctgattaatg
     8941 aacgccagct ctccgtcaat caacaaaact ggctgcgtca ttattttaag cagtatctgc
     9001 gtcagcacat tacgccgatt ttaatcaatc ctgacactga cttagtgcag ttcctgaaag
     9061 atgattacac ctatctggcg gtggaaatta tccgtggcga taccatccgt tacgcgctgc
     9121 tggagatccc atcagataaa gtgccgcgct ttgtgaattt accgccagaa gcaccgcgtc
     9181 gacgcaagcc gatgattctt ctggataaca ttctgcgtta ctgccttgat gatattttca
     9241 aaggcttctt tgattatgac gcgctgaatg cctattcaat gaagatgacc cgcgatgccg
     9301 aatacgattt agtgcatgag atggaagcca gcctgatgga gttgatgtct tccagtctca
     9361 agcagcgttt aactgctgag ccggtgcgtt ttgtttatca gcgcgatatg cccaatgcgc
     9421 tggttgaagt gttacgcgaa aaactgacta tttcccgcta cgactccatc gtccccggcg
     9481 gtcgttatca taattttaaa gactttatta atttccccaa tgtcggcaaa gccaatctgg
     9541 tgaacaaacc actgccgcgt ttacgccata tttggtttga taaagcccag ttccgcaatg
     9601 gttttgatgc cattcgcgaa cgcgatgtgt tgctctatta tccttatcac acctttgagc
     9661 atgtgctgga actgctgcgt caggcttcgt tcgacccgag cgtactggcg attaaaatta
     9721 acatttaccg tgtggcaaaa gattcacgta tcatcgactc gatgatccac gctgcgcaca
     9781 acggcaagaa agtgaccgtg gtggttgagt tacaggcgcg tttcgacgaa gaagccaaca
     9841 ttcactgggc gaagcgcctg accgaagcag gcgtgcacgt tatcttctct gcgccggggc
     9901 tgaaaattca cgccaaactg ttcctgattt cacgtaaaga aaacggtgaa gtggtccgtt
     9961 acgcacacat cgggaccggg aactttaacg aaaaaaccgc gcgtctttat actgactatt
    10021 cgttgctgac cgcagatgcg cgtatcacca acgaagtacg gcgggtattt aactttattg
    10081 aaaacccata ccgcccggtg acatttgatt atttaatggt gtcaccgcaa aactctcgcc
    10141 gtctgttata tgaaatggta gaccgcgaaa tcgccaacgc gcagcaaggg ctgcccagtg
    10201 gtatcaccct gaagctaaat aaccttgtcg ataaaggcct ggttgatcgt ctgtatgtgg
    10261 cctccagctc cggcgtaccg gttaatctgc tggttcgcgg aatgtgttcg ctgatcccca
    10321 atctggaagg cattagcgac aacattcgtg ccatcagtat tgttgaccgt taccttgaac
    10381 atgaccgggt ttatattttt gaaaatggcg gcgataaaaa ggtctacctt tcttccgccg
    10441 actggatgac gcgcaatatt gattatcgta ttgaagtggc gacaccgctg ctcgatccgc
    10501 gcctgaagca gcgggtgctg gacatcatcg acatattgtt cagcgatacg gtcaaagctc
    10561 gttatatcga taaagaactc agtaatcgct acgttccccg cggcaatcgc cgcaaagtac
    10621 gggcgcagtt ggcgatttat gactacatca aatcactcga acaacctgaa taaccctatg
    10681 ccaatacacg ataaatcccc tcgtccgcag gagtttgctg cggtcgatct tggttcaaac
    10741 agttttcaca tggtcatagc ccgtgtggta gatggtgcca tgcagattat tggccgcctg
    10801 aaacagcggg tgcatctggc ggacggcctg gggccagata atatgttgag tgaagaggca
    10861 atgacgcgcg gtttgaactg tctgtcgctg tttgccgaac ggctacaagg gttttctcct
    10921 gccagcgtct gtatagtcgg cacccatacg ctgcgtcagg cgctgaacgc cactgacttt
    10981 ctgaaacgcg cggaaaaggt cattccctac ccgattgaaa ttatttccgg taatgaagaa
    11041 gcccgtctga tttttatggg cgtggaacat acccaaccgg agaaaggtcg caaactggtt
    11101 attgatattg gcggcggatc tacggaactg gtgattggtg aaaatttcga accgattctc
    11161 gttgaaagcc gccggatggg ttgtgtcagc tttgcccagc tttacttccc tggcggggtc
    11221 atcaataaag agaattttca gcgcgctcgt atggcggcag cacaaaaact ggaaacttta
    11281 acctggcaat tccgtattca gggctggaac gtggcaatgg gcgcttccgg taccataaaa
    11341 gccgcccatg aagtgttaat ggaaatgggc gagaaagacg ggataattac cccggaacgt
    11401 ctggaaaaac tggtaaaaga agttttacgt caccgtaatt tcgcatcgct gagtttaccg
    11461 ggtctttccg aagagcggaa aacagtcttc gttcctggac tggcgatttt atgcggtgtg
    11521 tttgatgctt tagccatccg tgaactgcgc ctttctgacg gggcgttacg cgaaggcgta
    11581 ctgtacgaaa tggaaggacg tttccgtcat caggatgtgc gtagtcgcac cgccagcagc
    11641 ctcgccaacc agtatcacat cgacagcgaa caggcccgac gggtgctgga taccactatg
    11701 caaatgtacg aacagtggcg ggaacagcaa ccgaagctgg cgcatccgca actggaggcg
    11761 ctactgcgat gggccgccat gctgcatgag gtcgggttga atatcaacca cagcggtttg
    11821 catcgccact ccgcttatat tctgcaaaac agtgacttgc cgggttttaa tcaggaacag
    11881 cagctgatga tggcgacact ggtgcgctat caccgtaaag cgattaaact cgacgatctg
    11941 ccgcgcttta ccttgtttaa gaagaaacag ttcctgccac tgattcagct attgcgcctt
    12001 ggcgtattac tcaacaatca acgtcaggca accaccacac cgccaacatt gacactgatt
    12061 actgatgaca gtcactggac actgcgtttc ccgcatgact ggtttagtca gaatgcgttg
    12121 gtactgcttg atctggaaaa ggagcaagaa tactgggaag gcgtggctgg ctggcggttg
    12181 aaaattgaag aagaaagtac acctgaaatc gccgcttaat gctttgcggg ccgacgagaa
    12241 atgtcggccc gcattattca ggcactttcg cgaatgggtt cgatttcatt caacgtatca
    12301 attaacggct gcggcttacc aataagataa ccctgcatat aatcgatccc caaagagagc
    12361 accgcctcgc ggatctcttc gttttcaacg tactctgcca ctacccgcat tttcttcatt
    12421 cgcgccagat ggcaaatcga cgccacaatc tgataatcca gactatttga cacaatattg
    12481 cggataaagc tgccgtcaat tttaagcaga tcggcattca catttttaag ccgtgcatag
    12541 ctggcgtagc cggtgccgaa atcatcaatc gcaatctggc agcctaattc ctgaagatgc
    12601 tgcaaggtga tttgcgcctg cttaacattg gtcagagcat tactttcggt gacttcaaaa
    12661 ataagttgcc acgcttcaat ctgatattta gccagcaact gactgacttc aacaggaaaa
    12721 cgagcctgac ataccgaggt tggagacaga ttaatagcaa aacggtgagc gggcatcttc
    12781 gctctgtttt cagccataaa ttgcagcgta tgctcaatga cccacatgtc gatactcgac
    12841 gataaaccaa attcgtgcgc gaccggcaag aagctatcgg ggacgatcag ttcatcattc
    12901 tcacctttca tgcgcagaag aatttcatgg taaacatcac cacgcatacc ggtaatcggc
    12961 tgggccatca ggaaaaaatg gttgtgttcc agcgcctgct gtagtcgatt catcatcgcg
    13021 actttatctt tcaattcgcg ttgcaaatac attgccccgc gacgctgcat attttccggg
    13081 gcgttggtca cgatggaaag ttcggcgacc gtatttagct ctcccagcag caggtagata
    13141 tgattcactg gcgagcgcac atagcagtaa ctgacgccaa tctgcggttg catcggcatc
    13201 ccatcccaaa agaaacgaaa ttgcttgaga tgactatcca gtgcggtaat gcgctcctgg
    13261 tgcgattctg tattcagtcg cagcgcgaga tcgttacccg aaagctgata aacatcttca
    13321 cccggttcca gcaagggtga cagccagtga gaaagttttt gcttgtattg aatccgcagc
    13381 atgatgccat agttcttaac cagcatttcc atgccaggga tgcgtaaata acaaagcgca
    13441 gaccagggag catcacgcaa cgcgcgattc agggcgcgaa catttggcag atgaaccacc
    13501 gggtccatat acgcaagccg ctgaatgcgt ctgactaccg ctctctgacg ggttgccagt
    13561 acagccatat aattgacaat aaaagagaat accagataac tggaggaggt tatggtcagc
    13621 tgcgtggtat agccaggata aatgggaatg taattttgat agctgtggat gctgatcatc
    13681 aacacgaccg cccagagcag cgaaatcagc ttataaccat agcgcatcgc tccccacatc
    13741 atcaggggca gcaataatga caaggtataa ttggtgctaa aaattgtgct tttttcattt
    13801 aacggcatgc ataacagcaa caataaagca cctaatgcca gtagccagag cgcgaactct
    13861 tttttggtga ctttggcatc aacctgctgt tttaattgcg aataatagct acgtaaataa
    13921 aatggatttc gcactacccg aatgatgaag tagcacagcg ggacaccgat cagattaccc
    13981 accagtaagg cctgataatt gattaaagtc ccgaggttaa aaggcatgac accgaccaga
    14041 ttttctctgc tcgccagtaa tcctacaaat gcagcaaact ggaaaagtat cagaaacagc
    14101 gttgcaggaa acacaatctg ccagaatata cgctgggaaa ttaaacgggt atcaccatgt
    14161 gaaacgttgt tacgccgggg agtaaagacc ctgtaaccgc cccaacagag aatgataatg
    14221 ataataaaat tagccgttaa tgatagcgtt tcgtaaaaac ccagctctgc atatttgcgg
    14281 acaaaaatcc ctaaggcgat ccctggcaat gccgcccagc tgaaaaacat catcatgctg
    14341 atcatcagtg ccagaggcag ataaaagaga aaaacctcac cggaagaaat atgcgcgaaa
    14401 gtattaatgt gggcgaaaat gggcaagatt aaagaaggca ggaacagcgg aagcccccac
    14461 catttatcac gcatttttat ataagttgca ttcagtttca tagatgctca gcagaatccc
    14521 ccacatcctg aaggaggtgt attcagacag gcatcccacc tgacttcgaa tgatgattat
    14581 tcatcactat agagagcatt gattctaagt gtcatatgaa agttccaatt gatatatatc
    14641 aaacaaaata accctgatta atgaattatt acgtttatca tgttaattca tcattattac
    14701 atcatcattg taaataatta aattaacttc cataacatta aaatatgtat ccactgacgt
    14761 ttttttacat aacgaagaat tgaccatttt gtcctgttgt gccttaatgt aagtaccgtc
    14821 cacagcgtgg gacatacttc aaggaacctt ttgtgagtca ggcaaccagt atgcgaaaac
    14881 gacaccgatt taacagtcgc atgacccgta tcgtactgct catcagcttt atcttcttct
    14941 ttggccgttt tatctactcg tccgtcggtg cctggcagca ccatcagagc aaaaaagaag
    15001 ctcagcaatc cacactctcc gtcgaatcac cggtacaacg ttagcggtta ccttcttcac
    15061 tttcacagaa cataacggca cttcgctgtc ggatgctttt gctctttggg attatcaaag
    15121 cggcagatat tctttcatct taaattttac gtctttgtcc tgactgatgt ttatcctgtt
    15181 tggctgcgga ataaatataa aattaatata tatgttgtaa tgatatattt ttataaatta
    15241 ttccctgcgt gaattttaat aaatttaatc tatcccttta tacgcaatac atttactttc
    15301 ctcttttgat gatcttaaat gtcttatttt tcgtaatgtg tataacaagg aatagtgatg
    15361 aaatttaaaa aatgtcttct gcctgtggca atgttagcgt cattcactct ggcaggatgc
    15421 cagtcaaatg ctgacgatca tgccgccgat gtttatcaaa ccgatcaact gaataccaaa
    15481 caagaaacta aaaccgttaa tattatttcc attcttcccg caaaagttgc cgtagacaac
    15541 tcccaaaata aacggaacgc acaagccttc ggcgcgctta ttggcgcagt cgctggcggt
    15601 gttatcggcc acaacgtcgg ttctggcagc aattccggaa cgacggcagg ggcagttggc
    15661 ggcggagctg taggcgcggc agcgggttct atggtgaatg ataaaacctt agtggaaggt
    15721 gtttctttaa catataagga aggcaccaaa gtgtatacct ccacccaggt gggtaaagag
    15781 tgccagttta cgacaggttt agccgttgtt attaccacaa cgtataacga aacgcgtatt
    15841 cagccaaata ctaaatgtcc tgaaaagagc taataatcag gaggagtcat gaagaaagtt
    15901 tttctttgcg ccatcttagc ctccttaagc tatccggcta tcgcctcatc attgcaggat
    15961 caactctctg ctgtcgcaga agcggaacag caaggtaaaa atgaagagca aaggcagcat
    16021 gacgaatggg tcgcggagcg caacagggaa atccagcaag agaagcaacg tcgcgcaaat
    16081 gcccaggccg ccgctaacaa aagagcggca acggcagcgg caaataagaa agctcgtcag
    16141 gataaactgg acgccgaagc cactgcggac aaaaaacgcg atcaaagtta tgaagatgag
    16201 ctacgcagct tagagattca gaaacaaaaa ctgacgctgg cgaaagaaga agcccgcgtt
    16261 aagcgagaaa acgaatttat cgatcaggaa ctgaagcaca aagctgcgca aaccgatgtg
    16321 gtgcaatctg aagctgacgc caacagaaat atgactgaag gcggtcgcga tctgatgaaa
    16381 agcgtgggca aagcagaaga gaacaaatcg gacagctggt ttaattaatc gatgttagta
    16441 acttcaagcc tatgattctt gaagataaaa aaccctctgt agtaacagag ggttttgttc
    16501 attcatagtg cagggattaa aatcattccc actcaatggt agctggcggc ttgccgctga
    16561 tgtcatacac cacgcgggaa ataccgttca cttcattgat aatgcggttg gaaacgcgac
    16621 cgaggaaatc gtacggcaga cgcgcccagt gtgcggtcat aaagtcgatg gtttcgacag
    16681 cacgcagaga gacaacccag tcatacttac gaccatcgcc cattacgcca acggaacgta
    16741 ccggcaggaa cacagtgaac gcctggctga ctttgtcgta caggtccgct ttacgcagtt
    16801 cttcaatgaa gatggcgtca gcacggcgca gcaggtcaca gtactctttc ttcacttcac
    16861 ccagcacacg aacgccaagg cctggtcccg ggaatgggtg acggtacaac atgtcgtacg
    16921 gcaggcccag ctccagacca atcttacgca cttcgtcttt gaacagctct ttcagcggtt
    16981 caaccaggcc catcttcatc tctttcggca ggccgcccac gttgtggtga gatttgatga
    17041 cgtgtgcttt accggttgca gacgccgcag attcgataac gtcagggtag atggtgccct
    17101 gcgccagcca cttcacgtct tccagtttca gcgcttcttc atcgaatact tcaacgaaaa
    17161 cgcgaccgat gattttacgt tttgcttccg gatcgttttc gccagccagc gctgacagga
    17221 agcgatcttc tgccggaacg tgaacaatgt tcagaccaaa gtgatcgcca aacatatcca
    17281 gaacctgctc tgcttcgttg agacgcagca ggccgttgtc gacgaatacg caagtcaggt
    17341 ttttaccgat agcgcggtgc agtagcattg cggttacgga ggaatccacg ccaccagaga
    17401 ggccgaggat gactttatcg tcgcctacct gctcacggat gcgggctaca gcatcgtcga
    17461 taattttcgc tggcgtccac agggcttcac actggcagat atcacgcaca aaacgctcca
    17521 gcatgcgcat accctggcgg gtgtgagtca cttccgggtg gaactgtacg ccatagaagc
    17581 gtttttcttc gttagccata atggcaaacg ggcagctttc ggtgctggct acggtgacga
    17641 agtcggacgg gatagcggta actttatcgc cgtggctcat ccagacatcg agcagcggtt
    17701 taccgtctgc ggtcagcgca tcttcgatac cgcgaaccag tgcactgtcg tttacgactt
    17761 caacctgcgc gtagccaaat tcacgttcgt tagaggcttc aacgtgaccg cccaactgca
    17821 ttgccatggt ctgcatgcca tagcaaacgc cgaataccgg tacgcctgct tcaaagacat
    17881 actgcggcgc acgcgggctg ttttcttcag tagtactttc cgggccgccg gaaagaataa
    17941 tgccgcttgg attgaagtca cgaatttgtg cttctgtcac atcccacgcc cacagttcgc
    18001 agtaaacacc cagctcacgc acgcggcgcg caaccagttg agtgtactga gaaccgaagt
    18061 ctagaatgag gatgcgatgc ttatgaatgt tttccgtcat tgacgcttat tccgaggcaa
    18121 gtgaaacaga taatataaat cgcccgacat gaagtcgggc gaagagaatc aggagcccag
    18181 acggtagttc ggggactctt tagtaatggt cacgtcgtga acgtggcttt cctgaatgcc
    18241 cgcaccgctg atacgtacaa actccgcttt agtacgcagt tcgtcgatag taccacagcc
    18301 ggtcagaccc atacaggagc gcaggccgcc catctgctgg tgaatgatct ctttcaggcg
    18361 acctttataa gctacgcgac cttcgatacc ttccggcacc agtttgtcgg cagcgttatc
    18421 gctctggaaa taacggtcag aggaaccttt ggacatcgcg cccagggaac ccataccacg
    18481 gtaagatttg taagaacggc cctggtagag ttcgatttca cccggagatt cttcagtacc
    18541 cgccagcatg gaacctacca tcaccgcgct tgcgccagcg gcgatagctt tggcgatgtc
    18601 gccggagaag cgaataccac catctgcgat aaccggaata ccggtgcctt ccagggcttc
    18661 aactgcgtca gcaacggcag tgatctgcgg aacacctacg ccagtaacga tacgagtcgt
    18721 acagatagaa ccagggccga taccgacttt aaccgcgctg caaccagctt ctgccaaagc
    18781 gcgtgcacct gcagctgttg ccacgttgcc gccgatgatt tgcagatccg gatatttagc
    18841 acgggtttca cggatacgct gcaaaacgcc ttctgagtga ccgtgggagg agtcgatcag
    18901 cagaacgtca acgccagcgg caaccagcgc gtcaacacgc tcttcgttac ccgcacctgc
    18961 gccaactgca gcaccaacac gcagacggcc ttgctcgtct ttacaggcgt tcggtttacg
    19021 ttccgctttc tggaagtctt tcacggtgat catgccgatc aggtggaatt cgtcatcaac
    19081 caccagcgct ttttcaacgc gtttttcgtg catttttgcc agcaccactt cacgggcttc
    19141 accttcacgc acggtgacca gacgctcttt cggcgtcatg taaacgctaa ccggctggtt
    19201 caggtcggta acaaaacgca cgtcacgacc ggtgataata cccaccagtt cgttttcttc
    19261 ggtaacgacc ggatagcccg caaaaccgtt acgctcggtc agttctttca cttcgcgcag
    19321 cgtcgtggtt ggcagaacag tctgcggatc agtcaccaca ccagattcgt gttttttcac
    19381 acggcgaact tcttctgcct ggcgttcaat ggacatgttt ttgtggataa agccgatacc
    19441 gccttcctga gccagagcaa tagccaggcg cgcttccgtt acggtatcca ttgctgcgga
    19501 aagcataggg atattcagac gaatagtttt cgtcagctgg gtgctgaggt cagcagtatt
    19561 cggcagaacg gtagaatgag caggaacgag gagaacgtcg tcaaacgtca gagcttcttt
    19621 agcgatacgt agcatgggca atatctcgac cagagtggtt aataaatatt gccgcggcat
    19681 tatacagagc gtaaccgatt gcatctaccc ctttttgcaa aaaatgcttg ctatccccga
    19741 agggcgggtt actatcgact gaataacctg ctgatttaga atttgatctc gctcacatgt
    19801 taccttctca atcccctgca atttttaccg ttagtcgcct gaatcaaacg gttcgtctgc
    19861 tgcttgagca tgagatggga caggtttgga tcagcggcga aatctctaat ttcacacaac
    19921 cggcttccgg tcactggtac tttacactca aagacgacac cgcccaggta cgctgcgcga
    19981 tgttccgcaa cagcaaccgc cgggtgacct tccgtccaca gcatggacaa caagttttag
    20041 ttcgcgccaa tattacgctc tacgagccgc gcggtgacta ccagataatc gttgagagta
    20101 tgcagccggc cggtgaaggg ctgctgcaac agaagtacga acagctcaaa gcgaagttgc
    20161 aggctgaagg tttgttcgat cagcaataca aaaaaccact tccctcccct gcgcattgcg
    20221 ttggtgtgat cacctcaaaa accggtgctg cgctacatga tattttgcat gtgttaaaac
    20281 gtcgcgatcc ttctctaccg gtgatcatct accccaccgc cgttcagggc gatgacgcac
    20341 cggggcaaat tgttcgcgcc attgaactgg cgaatcagcg caatgagtgc gacgtgttga
    20401 tcgttgggcg cggcggcggt tcgctggaag atttatggag ttttaacgac gaacgcgtag
    20461 cgcgggcgat ttttgccagc cgcattccgg tcgtcagcgc cgtcgggcat gagacggatg
    20521 tgaccattgc cgattttgtt gccgatctgc gtgcgccaac gccgtctgcc gccgctgaag
    20581 tagtgagccg taatcagcaa gagttactgc gccaggtgca atcgacccgt caacggctgg
    20641 agatggcgat ggattattat ctcgccaacc gcacgcgtcg ctttacgcag atccatcacc
    20701 gattacagca acagcatccg cagctccggc tggcacgcca gcaaaccatg cttgagcgcc
    20761 tgcaaaagcg gatgagcttt gcgctggaaa atcaacttaa gcgtaccggg caacagcagc
    20821 agcggttaac acagcggctg aatcagcaaa atccacagcc gaagattcat cgcgcgcaaa
    20881 cgcgcattca gcaactggaa tatcgtttag cagaaaccct gcgcgtacag cttagcgcca
    20941 cgcgtgaacg tttcggtaat gcagtaacgc acctcgaagc cgtaagccca ctgtcaacgc
    21001 tggcgcgtgg atacagcgtt actactgcta ctgacggcaa tgtactgaaa aaagtgaagc
    21061 aagttaaagc gggtgaaatg ctaaccacac gtctggaaga cggctggata gaaagtgaag
    21121 ttaaaaacat ccagccagta aaaaaatcgc gtaaaaaggt gcattaagcc agcacgaatt
    21181 caacgcgttt tttcgatatc aagccatgcc cgtgctgaca gaagtaatct actgcaccgc
    21241 aggcttttaa cacctgcaac ggttgatggc agtctggaca aagagctttc atttcaataa
    21301 tttccccgca ggatgggcaa cgcgcatggc cgttatcctg atcaagcaca tgctgacatt
    21361 gtggacaatg cagttccata tgaattcctc cggtagccat ccctctacat tcatagaggg
    21421 aatggcagat aaaatactta cggataatta tttatttttc ttgatgtgct tcatcagacg
    21481 cttacgttta cgcatctggg ttggcgtcag ggtgttacgc ttgttcgcat acgggttttc
    21541 cccttctttg aactgaatac gaatcggcga tcccattacg tccagcgatt tgcggaagta
    21601 gttcatcaag tagcgcttgt aggaatcagg caggtctttc acctgattac cgtgaatcac
    21661 cacaatcggc gggttataac caccagcgtg ggcatatttc agcttcacac gacgaccgcg
    21721 taccagcggc ggttggtgat cttcaacagc catcgtcatg atgcgcgtca gcatagaggt
    21781 ccccacacga cgggtggagc tgtcatacgc ttcacgtact gattcaaaca agttaccaac
    21841 accactgccg tgcaaggcag agataaagtg cacacgagca aaatcgataa agcccagacg
    21901 gaagtccagc gtttctttca cctgctcttt cacttcctgg ctcaggccat cccacttatt
    21961 caccacaatg acaagtgagc gcccactatt gagaataaag cccagcagcg agagatcctg
    22021 atcggaaata ccttcgcgcg catcaatcac taacatcacc acgttggtat cttcaattgc
    22081 ctgcaacgtt ttgattacgg agaatttctc tacagcatcg gtgattttgc cgcgtttacg
    22141 tacgccagcg gtgtcaatga gcacatactc acgtccatcg cgttccattg gaatgtagat
    22201 actgtcacgc gtcgtgccag gcatgtcata aacaacaacg cgctcttcac caagaatacg
    22261 gttagtgagt gtagacttac ctacgttcgg acgacccaca atcgccagtt tgatcggcag
    22321 actttgcggg tcgaagtcgt cttcctcttc ttcttcgccg ttctcttccg cttcaaattg
    22381 cgcccagtat tcagcgtctt cgtcgacttc ctcttgcggt gcgagatctt ccatccacgg
    22441 cagcagcaca cgctccagca gacttaatac gccacgaccg tgagatgcgg cgatcgggta
    22501 gatttcacct aaaccaagcg agtagaaatc aaccactgcc tgatcgggat ccagaccgtc
    22561 agttttgttt gccaccagga aggtcggttt ttcacgagag cgcagatgtt tggcaatcgc
    22621 ttcatctgcc ggcatcaagc ccgcgcgcgc atccaccata aacagtacga cgtccgcttc
    22681 ttcaatcgcc agcagcgact gttccgccat gcgggtttct acaccgtctt ctgtgccatc
    22741 aatcccgccg gtatcaatac agataaactc acggccttca atttccgcac gaccgtactt
    22801 acggtcacga gtcagacccg ggaaatccgc aaccagcgca tctcgggtgc gagttagacg
    22861 gttaaataac gtggattttc ctacgttagg gcgcccgaca agcgcgacca caggtaccat
    22921 gtttaaagcc tcatttttat aaatcatcag acaacgcacg ctatattcgc gtcgttgtta
    22981 aaaacaggaa aacggcccct gtccaggagc cgttttcaaa gtgaacgaca gagacgatta
    23041 acgtgtaata gagtacacgg ttccgtcttt tgcctggatc agcagtttgc cgtcagcggc
    23101 aaccggttca gtctggaaac cggaactatc aactttttgc tgggcaacga aacgaccatc
    23161 ttcgacgtta atccagtgca gataaccttc actgtcacct accaccaggt tgccattata
    23221 cagcaccggc gaagtcagca ggcgatgcag cagatcgctt tgtgtccaca gcgtaacgcc
    23281 gccatcaatg gtcaacgcca tcacccggtc attttgatcg accagataga tgcgattgcc
    23341 gtcgacgatg aaatcattca ccgaacccag ttcgcgtttc cacataatct gaccactgcg
    23401 cagatcaagc gccgtcaggt taccattata ggccagagcg aaaacaacgc cgttaacaac
    23461 gacgggagtc gtgtcaacat cgctcagacg gtcaatttcg gtagaaccgg tcgcctggga
    23521 aatacgctgc tgccaaatca tctggccctg ttccatcagc actgcgctga cgcgaccatt
    23581 atcgcccccc acgacggccg caccaaaagc cgttgccggc gcagactcgc cacgcaaaga
    23641 gagcgaaggc atatcgaggt taactgtcca tttgacagcg ccgtcagctt cgttcagcgc
    23701 ttgtaactga ccgttactag tgtggattaa caccagaccg tcgctgacca ccgggcgaga
    23761 aagtgcttca cccgcgactt tagtttgcca tgccacagta ccatcgctgg tattcagcgc
    23821 gtaaacctgc gccttttcgc tgccaatgta gacatgccca ccagacacgg tcacaccgcc
    23881 agaaagtaat gcaggctctt tagagaacca gccatctttc tcggccaggc tgacagacca
    23941 gatttctttg ccatcatccg cattcagcgc ttttaccaaa ccagcgcggt ccgctgcata
    24001 gacaacgttg tccgccagtg ccggatgaag attggaatag aagttgccaa tgccgctacc
    24061 aacggaagtg ctccacgccg tggtcggcgt aaactggttt tcaacggttg gcaatgggga
    24121 catctttacc acatcttctt cgctgttaaa cagcgaacag ccgcttaaaa gggtaacgga
    24181 aagcagtcct ggcagcagta atttacgcaa ttgcatcggg tccctctcag atggacaaat
    24241 tattaatttt catctgcatc atttcgctca gtgccggagt aacatcgctt ttcacgcctg
    24301 cttcccatgc actacgcgca ccttgcttat cacctttgct caacaatgct ttaccacgca
    24361 ggtcggcaac aatggcagcc cacccttcac ctttgatggt atcaagggtt ttcagcgcgg
    24421 catcagcctg cttgagctgt acctgaacgc gagcaagacg cagatttatc acggctttga
    24481 gattttcatc actcgtgtct gccagcccct gttgtaactg ggcggcagct ttctctagtt
    24541 catttttgtc aacaaattgc tgcgcaagtt ccaaagaagc cagcgcacca taagtatttt
    24601 tattttcagc agcaaatttt tccgccgccg ggatgctatc cggtttgcct tcgctcactg
    24661 cggtaaccgc attttgatag gcaagagaag cggagcgtgc agaatcaacc tgatggctgt
    24721 tccagtagcg ccagccaatc agtgcgccaa cgcccaaaat caccccaaca gccagtgctt
    24781 tgccattttc agcaaaaaag cgtttaaccg cttctacctg gtcgttttcg ttctcgtaaa
    24841 tttccacgct gtccttctcc ttccttaacc cagtaacgtg cgcaaatgcg cggctacgct
    24901 atcctgcgca actgccgttt gctcaccaga gcgcaaatcc ttcactactg ctgtgccgtt
    24961 agccacttca gactcaccca gcaccacagc aacgcgggca ccccatttat cagcacgggc
    25021 aaactgtttc ttaaagttgc cgccgccgtg gttggtcatc aatttcacgc ccggtaattc
    25081 atcacgcaga cgctcagcta atgccatagc cgcagattgt gtatcagcac ctgaagccac
    25141 caggtatata tcgacaacag gatcggcttt aaattccgga ttaacggcct gtactaacaa
    25201 tacaagacgt tcgaggccca tcgcaaaacc gactgccggt gttgcacgac cgcccagttg
    25261 ttccaccaga ccgtcataac gaccgcctgc acacacggtg ccctgggagc cgaggctgtt
    25321 agtcacccac tcgaaaacgg tacggttata gtaatccaga ccacgcacca gacgctggtt
    25381 tacggtgtaa gcgatccccg cgctctcaag cagtttgcac agaccggcaa aatgctcacg
    25441 agattcctcg tccagataat cacctaatgc cggagcgtcg ttgagaagcg cctgcacttc
    25501 cggatttttt gaatccagca cgcgcagcgg gttagtgtac atgcggcgtt tgcagtcttc
    25561 gtccagcttt tctttatgct gctcaaggaa tgccaccagc gcatcgcggt aattggcgcg
    25621 tgcttccagc gaaccaatag agttcagctc aagagttacg tgttcgaaga tacccagcgc
    25681 acgccaccag cgggcagtga gcataatcag ttcagcgtcg atatccggac cttgcagacc
    25741 gaaaacttcg cagcccaact gatggaactg acgataacgc cctttctgcg gacgctcgtg
    25801 acggaacatc ggcccgatat accacagacg ctgttcctga ttgtacagaa gaccatgctc
    25861 gatgccggcg cgtacacagc ccgccgtccc ttcagggcgc agagtcaggc tgtcgccatt
    25921 gcgatcctca aaggtgtaca tctctttttc aaccacgtcg gtgacttcac caatcgcacg
    25981 tttgaatagc ggggtctgct ctacaatcgg caagcggatt tcactgtaac cgtagctgcc
    26041 gagcacgttt ttcagtgtgc cttcaatgcg ctgccagatg gccgtttcgc caggcaggta
    26101 atcgttcatg ccgcgaatgg cttgaatgtt ttttgccacg tttattctct ttctgaatat
    26161 aaaaatgaac cctcaacgct tccctcaatg tttcgggagc catgcgggtt caatcataca
    26221 cgggaagcga ggcgcttccc atcacgttat tatttttcaa cctgctgaac gtcaattcga
    26281 cgcgcttcgt ccagctgact ggctttcgca cgaatgcgtg cttccagctg gtcgatcata
    26341 tcgttgttgt ccagacggtc tttgcgcacg ccatcttcat agaggccgct tttcttgttg
    26401 ccgccggtga cgccgagtgt agaaaccagc gcctcacctg ggccattcac cacgcagccg
    26461 ataatcgaaa cgtccatcgg agtgatgata tcttccaggc gttgctccag cgcgttaacc
    26521 gtaccgataa catcaaattc ctgacgcgaa caggtcgggc aggcgatgaa gttaatccct
    26581 cgcgaacgga tacgcagcga tttcaaaata tcgaaaccga ctttgatctc ttcgaccgga
    26641 tcggccgcca gcgatacgcg cagcgtgtcg ccgatgcctt ccgacagcag cagacctaaa
    26701 ccaatggcgg attttactgc cccgctgcgt gcaccaccgg cttcggtgat ccccagatgc
    26761 agcggctgat cgatctgttt tgccagcaaa cgataagact caacagcgag gaagacgtca
    26821 gacgctttca cgctgacttt gaactgatcg aagttcaggc gatcgagatg atcaacatga
    26881 cgcatggcgg attccagcaa cgcctgcggc gtcggttcgc catacttttc ttgcagatct
    26941 ttttccagcg atccggcgtt aacgccaata cggatcggaa tgtttttatc gcgcgcacag
    27001 tcaaccacca tgcgaatacg ctcttcatta ccgatattgc cagggttaat acgcagacaa
    27061 tcgacgccgt attccgctac tttcagcgca atgcgatagt cgaagtggat gtcagccacc
    27121 agcggcacgt taacccgctg tttgatgagt ttgaacgctt ctgccgcgtc catcgtcggt
    27181 acggagacac ggacgatatc agcgccaacg cgttccagcg ccttgatttg attgaccgtt
    27241 gcttcgacat ctgtcgtacg cgtattggtc atggactgta cggcgatggg agcaccatcg
    27301 ccaatcggca cattcccaac gtaaatacgt gttgattttc tacgttgaat tggagcctgg
    27361 ttatgcatga aaaatctccc gcgttacccg tctgttactg cgccggtgat tgttcggcat
    27421 tgagggtcag acgcgcaacc tggttagttc tgataaaacg actcagatcg acaggtttcc
    27481 cttgatactg gatctgtact gcggctggcg caccaatttt cagtttatac ggtgcctggc
    27541 cggttaaatt caaattaccg tctttacgct gcatcccgct aaacaatttt ttaccggtag
    27601 catcagtgac ctccagccag caatcggcag taaagttcat caccagcgca ttcggatcag
    27661 ccgccggcgt ggtcacgcca gcctgatcgg ttggcaacgg cgcagcacca tctggcgttg
    27721 ttgctgccgt tggtaccggg gtcgcggcgg tatcaacatt tgcctgcgaa ggcgaaacaa
    27781 ccgcattctg ttgcggatca acagctggtg ctggcgcagt tacggcaggt gtttgcgtgt
    27841 tggttgcggt agtatccaca gacgctggcg gcgtggacgt tgtagccggg tctgtagttg
    27901 tcgacgtatt taacgggaca ctctgcccct gctcgctatt actgctcagt tctgccgaag
    27961 attgatcggc catagtggtg atctcttcct gctgagcttt gtggtcttgc caccaccagg
    28021 caccgctcag gccgataacc acaaacaaca ccagccaggt gaaggtcatc agccagccat
    28081 cacgtttttt gcggcgttta ccgagggaaa aactctgcat tggcgcaact tttgcagccc
    28141 gtagtggagc ctgcttttcc agccctggca gcagttcttc ttctggaata tgtaccagac
    28201 gcgcataaga gcggatatat ccgcgcagga atgttgaagc aagatcggcg ggtgccttat
    28261 cttcttcaat gtcgcgtacc gtggaaacct tcaggcaaag tcgctcggca acggcctgct
    28321 gactaagtcc tagttgttcg cgagcattac gcaggcgagc gccggtagta agtgcttcat
    28381 tttggtcgtg cgtggcttca gtattcattc gctgcaggta cgtttaaatg agaattgaga
    28441 tgccggtgaa ccatcatgct cacccacacc gcgaaacatc cgttaagtta accgttatca
    28501 tacagtataa gaccgtctgt gtgctcgtga caaagcccac attcactaag gctaacttac
    28561 tgttgcatcg ttacatactg ccttaaagtc agcaaaaacg caccgttaat attgaccaga
    28621 caattgcaac ttaatacttc attcataaaa cattacgcca cggtacataa agtaaccgtg
    28681 gcgtaatggc tatcagaccg ctttaatgtc gatggcttca ccctgcatcc gtttacgcag
    28741 ggtacgtttc gtacggtcga taacatcgcc cgccaactga ccacaggcag catcgatatc
    28801 atcaccacga gttttacgca caatagtggt gaaaccgtag ctcatcagca cttttgagaa
    28861 acggtcgata cggctgttcg agctgcgtcc atacggcgca cccgggaacg ggttccacgg
    28921 gatcaggttg atcttacacg gcgtatcttt cagcagttcc gccagttggt gcgcgtgttc
    28981 agtgccgtcg ttaacgtggt caagcatcac gtattcaata gtgactcggc cctgattggc
    29041 gttggatttc tccagataac ggcgcaccgc agcaaggaac gtttcgatat tgtacttttt
    29101 gttgatcggc acaatttcgt cacgaatttc gtcgttcggc gcgtgcaggg aaattgccag
    29161 tgcaacgtcg atcatatcgc ccagtttatc cagcgccgga actacaccgg aagtagaaag
    29221 cgtgacgcga cgtttagaca ggccaaaacc gaaatcatca agcatgattt ccatcgccgg
    29281 aacgacgttg ttcaggttga gcagcggctc gcccatgccc atcatcacta cgttagtgat
    29341 tggccgctga ccggtgactt ttgctgcgcc gacgattttc gccgcccgcc acacctggcc
    29401 gataatttcc gacacccgca ggttgcggtt aaagccctgc tgggcggtgg aacagaattt
    29461 acactccagc gcacacccca cctgcgaaga gacgcagagc gtggcacggt cgtcttccgg
    29521 gatatacacc gtttcgacgc gctgatcgcc aacggcgatc gcccatttaa tggtgccgtc
    29581 ggatgaacgc tgttcttcaa ccacttccgg tgcgcggatc tccgccacct ctttcagttt
    29641 gccgcgcaac actttgttga tgtcggtcat ctcatcaaag ttgtcgcagc aatagtgata
    29701 catccacttc atcacctgat cggcgcggaa gggtttttca cctaaatctt taaaaaactc
    29761 ccgcatctgc tgacggttga gatccagcag gttgattttt ccatctttcg tggtgacgtt
    29821 ttcaggtgtg actaattgtt cagacatatg ctattccggc ctcgttatta cacgttatgg
    29881 cccctggagg gttgaaaaaa gaaacgcccc ggtgagcggc tgctcgtccg ggggcgctgc
    29941 attgtacaaa ttctggcgca cggatgccac gtttgcacgc ggcatttacg aaattattaa
    30001 cgggtgcgcg ggcacacttc gccttcgccg aagaaataag cgatttcgcg agcggcagat
    30061 tcgacggaat cagaaccgtg ggtaccgttt tcggtcaggc tgtcagcgta atcagcgcgc
    30121 agagtaccgg ccagtgcgtt tgccggattg gtcgcgccca gcagatcgcg gtgacgctga
    30181 acggcgtttt caccttccag cacggaaacc acgatcgggc cagaggtcat gaattcaacc
    30241 agaccatcaa agaacggttt tccatcgtgt tcagcataaa agccacgagc ctgttcaacg
    30301 gtcaggtgca gcattttggt gccgacaatt ttgaaccctg cagcttcaaa gcgcgcaaag
    30361 atattaccaa tgacgttttt tgctaccgcg ttcggtttga tgatggaaaa agtacgttca
    30421 atagccattt ttacctctgt aaattgttct gttgttgtct gtaccagcgt acgagatggc
    30481 gcggattata atgagcaaca gggccgttga ctattgatga aggtaacatt tttttaaaat
    30541 aaaatgagtt ttagcaacaa tcagggacaa aacagactat tgcatgacaa atttcactgt
    30601 cgcgatttgc cccacgtcat ccatcaccag caattgataa tcacctttat ccgtcaaatg
    30661 cagggtcacg ttgcgcccgc gttcagttaa cggttcgcca ttcagaaacc accagcgttc
    30721 acctgcccct ccacttgatt gcaacggcaa agtcgcttcc gctgcgcccg gtaaacgttt
    30781 aataatcgcg ccatcgcgaa cgcctgtcag ctgcagaggg agctgggcat cgtgaccgta
    30841 tggcgggcaa attgtcgagg ccggtggtaa gcgcacagcg cgacgttctg atgcgggcag
    30901 ccagggttcc agcggtagcg gccagacatt taccatttct tgcttcgcct gcgggcagtc
    30961 agcggcgacg cgctgccctt tgtcatccag ccagatgggg aagcgaatgc cattaatccc
    31021 ttcctgctct gacagtaaca gcgttggcgg ctggctgcca tccagcagcc aggttgccag
    31081 gcggcggcga cagttaccgt caccttccgg caaagactgt ccgcccggcc agcagataac
    31141 gccacgagtg actgagttcg ggcgcgggtc ttccggcaga ttcgcactgc gcgacagtaa
    31201 gatattattg acctgattca acaatggtac ggcactggca aagccaaact gaccaacaac
    31261 gggcgtgccg tcaggtctgc cagtccagat cccaatgaca tagcgagcgt taaccccaat
    31321 cgcccaggcg tcacgatagc catagctggt gcccgttttc catgccagtg gggcgacgcg
    31381 cggcaaggca ctatccggca agggttgcgc ttcatcagcc ataatccggc gaatgatcca
    31441 cgccgccccc gacgacatta aaggccgttc aagcagcgga tcgtcaggct gtaagcgcaa
    31501 tttgcctgcc ttgccgtggc gagcaaacgc ggtatacgct gccgccatat cttccagttt
    31561 tgcaccagcg ccgccgagaa tgagtgaaag attcggcgca gcgccgttgg gcaaatataa
    31621 cggcaatcca acattgcgta actttgccgc aaaccgtttc ggtccatagg cttccagcac
    31681 ctgcacagca ggtaagttca gcgagcgcac cagcgcctcg ctcatgctga tcgggccatg
    31741 aaaaccgcta tcaaagttac ctggtcgata atcaccggtg cgccggggga cgtcttgcag
    31801 cagtgatgcc gggtggatca agccttcatc cagcgccaga ccataaacaa acggtttgag
    31861 caccgatcct ggcgatcgga tcgcattgac catatcaaca tgaccaaagc gtgaatcatc
    31921 gttgagatca accgatccca cccagccgcg aacacgcata tcggtatgat caaccacgat
    31981 catcgccagt gagctgcgcg gtggcaaccg ccctttccag ttttgcgcca gttcttccag
    32041 acgtcgttga agaccggcat ccagcgtagt aacgattttg tcgcttttgc ttttaccgag
    32101 catcatgcgc gaaaacagcg gtgccagttg cggcatttgt cggggtgcca gccagatggg
    32161 ttcttccctt gactctttta cctgctcacg ggaccacaca ccttgcgcag ccatccgttc
    32221 gagcacttta ttacgcgcgg cttcggcacg ctccggccaa cgatccgggc gaagacggct
    32281 gggcgcttgc ggcaaaaccg ccagcattgc cgcctcggaa tagcttaaat tcgcaggcga
    32341 ctttccgaga taagcccaac ttgccgcacc gatcccctgc aacgtaccgc caaacggagc
    32401 gcggttaaga tacaaggtca gaatttcacg cttagacaga tgccattcca gttgcaacgc
    32461 gcgccagagc tggcgaattt tgccgccaaa tgttttgggg tgaggatcaa gcagacgagc
    32521 aacctgcata gtgagcgtgc tgccaccgga aataacccgt cccgaagtga gatcttgcca
    32581 tgctgcgcgc gccaccgaga atggattcac ccccggatgc ttccagaacc agcgatcttc
    32641 ataattgatc agcgcttcaa ggtaacgtgg agaaacatct tcgattgtta ccggataacg
    32701 ccagatgccg tcagcatcgg cgaagcgcca gagcggcgta ccatcctgcg ccacgaccac
    32761 tcgtgcggga ttgacttcat gcagcggtag aggccagagt ttatctgctc cccacgctgc
    32821 aagaaaaagg agaaatggcg cagctgccag cgttatccag cagccgcgtt tggttaacaa
    32881 gcgaggcatt tacggtcgga caatcagcag atcttcagcc gcgccggtcg cccgccattg
    32941 gggaacatac attgattcca ccatcggttg cggtacctga tacgttcccg gcgtcaccgc
    33001 ccgcgccaga tacaccagcg ttaccggttg gtattcatca acggcaaccg ccgccacaaa
    33061 gcgatcgtca cggaactcaa tgtgcttaat actcgcctgc tgcatctggt tcagtaagtt
    33121 ctgcacttcg ccaccacttt gctccaggct ggcgctaccg ttcgccagat tctggttttc
    33181 cagttccaga cccacaggca gcagatccac gactaacgca tccggcacgc tgttactggc
    33241 ttttacctgc aaccacacca gcaccagatc gccgctacgt aacgagtcca gcgatttgct
    33301 cttaccatca gtaccaagaa tatgacgctc gatttgcagc acattgttcg ccggtaaagg
    33361 tgcggattgc ggataaccgc tggcatccat acgcaaccat aacggctgat cgccactgtt
    33421 gctcacctgc aaggtgacaa gttgatcgct attcagattg ctgttttgcg ctttctcgcc
    33481 tgtcagcggc tcagctgaga aagaggtttg cgcctgccat ttacccggta aatcctgaat
    33541 cgtacgggca gccaggaaca acgcgttact ttcctgcgtc gatagccagc gttcaccaaa
    33601 cgcctgctgc gaaagtgtgt tcagcaaagt gtactgctca tcgggtagca gtttattttc
    33661 ttccagcaag gagagcatta acgcgttgtc gcgcagtgga ctaccgtaat cacccagcca
    33721 tatccgctca tcactatttc tcggcgtttt cagcgccagc gcaatcgctt cttcaccacg
    33781 cgtcgcatca cccatggttt tcagcgcaat gccaagttgc agcagcggta aaccagaagc
    33841 ggcatctgca cgatgctccc agatttcacg cagcgcaccc agtggtgctt tttgctgacg
    33901 ggccagcacc agcgcagcgt aagactgaac ggcgaattta ctggctttga gattatccgc
    33961 gtacgggatc gacatcatgc ccggatcttg taaatagcgc agcagacgct cattaccccg
    34021 gttaatggcg tctgtcggca cgctgtaacc ctgttcgcct gcgcggacca ggaaatccat
    34081 cacgtaagcc gtcagccagt actcttcgtc accgttttta tcccacagcg caaagccgcc
    34141 gttatcacgt tgcatttgca gcaaacggga aatgccgata tcgaccgatg cacggcgttt
    34201 ctcatcactg tcgcctttga tgcccaacgc ctgcagttgg gctgcgttgg tataaagtga
    34261 cgggaacagg ccgctggcgg tttgctcaag acagccgtac ggatacgctt ttaactcttt
    34321 gatataacgt gcaatgttca gcggtggttt gccgctcaac aacaattgcc cttccagcgt
    34381 aacaggcgag aagttttgca atccatccgc cggaatcgcc catgtctcac caggctgtaa
    34441 cgccgtaccg taattaaccg tttgtgccgg gaacgccgga cggacgccga ttttccactg
    34501 cttatgctga tcggcaacgg tttcacccgg taacgctaac ccgctaatgg tggcctgaat
    34561 ttctccatcg ccataacccg gcaatgctcg caccgggata aacagcgtag tacgcacacc
    34621 tggcgctaat tcaacggctg cgggtgaatc gctgaccagt tcaagcaaac cactggcggt
    34681 cagggcaacg ttcagttttt gcggtttatc ggtaagatta gtgatatcca gcgtcagacg
    34741 cgaggtatcg ccactcgcca taaagcgcgg catgttcagt tcagcaatca ccggtgcggc
    34801 aactatcact ttgctttcgt tgctaccgaa atcatctgcc gtccaggctt gcgccatgac
    34861 gcgcaattca ccgttaaaat cgccaatcgg cagtgtaacc gagccttcgc cctgttcgtt
    34921 gagcgttacc gacagcgcct gctgcgcgac aatattgacg tgattgaccg gcggtttacc
    34981 accacgtttc agctcatcac catcgccacc gaaacgcaga gctgccagac gcccctgacc
    35041 ttcaataacc tgaccgtaaa tatcgtaaat gtctgcgcca tagcgtttct gaccaaagaa
    35101 cgcctgccac ggatctggcg taacgtagtc agtaatattc agcacaccac tatcaacggc
    35161 agacaccagc acattcacct gtttaggctt ctcgccattt ttagtgctgg ctttaatttt
    35221 cacggttaat ggttgattgg gacgcatttt tgctggtgtt tccagcgcca gatcgaggcg
    35281 acggttttca tcaccaagcg gcagatgcaa cacaccaacc gcgcgttttg gcgtcgcgga
    35341 gcgagattta tcgccaggac gcaccaccag cgtactcaaa taaagatcat gacgattcca
    35401 ggttttatcg accggaatcg tcagatccag cccttgagcc ggaacatcaa tctcttgcca
    35461 ccacagcggc ccttcactgg actcgaccat cgcataacct ttacccgccg ttggcgcggc
    35521 aatatgcaac ttaatggtat cgccggggcg ataactggct ttatctagtt tcagcgtgac
    35581 acggtcgggt cgcactgcgc cgctcccgtc gctgttgtcc tgccagctat agccagccca
    35641 aaaacgcacg ctactgaccg cttcattcgg cgctttgact tccagacgat aagcgcccca
    35701 ctctaccggg aaactgacct taccggtttc atccgcctga agatccagag tttgttcatt
    35761 ttcgatcaga tctttttgat caaactgtga ctgccagcct tcatcttctg accagttcca
    35821 gtagtaatcg cggcgttcgc gaatcaggcg cacctgcaag cccgacacgg cttttttcac
    35881 gccttgcgca tcgctataaa cgatgtcaaa agcggcgtta ctgccttcat caacaatggg
    35941 ctgtttcacc gtgctgtcag tgcgataatc gtaaaccgat ttcaaggcga actgcggacg
    36001 gagccccggc aatgcatcgg caggccagat agcctgctca gcgcggcgcg tcaccgggcg
    36061 accgcccgat tccagcaaac tgccctggaa aataacctgc aatggggaat gcgtttcctt
    36121 ccactggctt tctgtagaaa cttcgccgcg ccctttatca tccagcgtca actgaacttc
    36181 atccagcgtg cgggaaaggt tttcggcagc tatatcgccg aattcaaaac caggtaaggc
    36241 tgacacagcc tcacgcagtg ggcgcaggaa aagttgccct tgcaaagtat taccattagc
    36301 aggcgcaccg tacaggtagt atcccaccac ggagaatttc acttcatcat tcggcgtaag
    36361 cggggttttc tcaccggtca gattcagcgc catacgctct ggcataaaat cttcgacgtg
    36421 gaaatcccac atccggtact ggttatcgcc cgtgttggcg cgaatatgcc acataccggt
    36481 tgccgcattg ctatcgagtg gccaggtaaa gtggtagagg ccattctccg gctgactaac
    36541 gacgttcctg agtacctgcc cgtcaggttt aatcacgtct aacttgatgg gttgatcggg
    36601 caatgcttta ccgtctgcat cgcgcagtaa accattgagg attaccgttt cacctgggcg
    36661 ataaagatcg cgtgggccaa acatgaaaaa ctgtttgcta tagcctggcg cgccagcaat
    36721 gttaaattct gctaagtcca gcgccggaag ttttaaatcg agtagcgttg tctgaccgtc
    36781 tttacgtgcc agcagtaatg ccgcattttt atcattttcc agctgcacat gcccctgagc
    36841 gtcactggtt gcctgagtca gagtctgtcc tttctcattt aataaagaga cttcaattcc
    36901 ttgctgggcc gcgccgtttt ccagactttg ggtaaagata tccagacaat tgtgataacg
    36961 gtgagctgaa acgccgatat cacttaacgt aaacagcgtc gcgggattac tgtaatcgta
    37021 acgtccagcc tgattcatca cagccagata cacgcccgcc tgctgaagcg gtttgatatc
    37081 gcccagcggc agcaataatt tttcacgggt gttacgcgca ggattgagat caaaccgtcc
    37141 ggtgtagacc agatccgcca tctgcagcag tttgtctgac tgccagttcg ccagcgaatt
    37201 gcggtattcc cattggctaa tgaatgctgg cagagattct ggcttaacgc ggaagaagtt
    37261 aacatcgaca ttattaacgt tgagcgccat taccggcagc ccttcaacga ctttgccagg
    37321 cagcagcgaa ccacggctgg caaaaccgac gctgggttgg atgtcgcggg tagttatagt
    37381 tttttcgtaa tctttactga aggttgcgtt gttgagtgct ttgacctcct tgccaatagt
    37441 aacgatcaaa tcacgtttcg gttcgaggtg gcgtaaacgt agctctttaa gattatctga
    37501 cagctcccag gcaccatcca ctttgccgct ttttttatcg acgacatgaa taacgcgtga
    37561 gaaatcctga tccgggtcga gagggatgga gaacgtcagc accagcgtgg ctgcaccatc
    37621 aagttggact tcagagagat ccagcaatgt cagcacctta ccggcactct gttgggccag
    37681 tttttgtctc tccggcacgg agagttttgc tgaactcgcg ttttctgaag aggccgcttt
    37741 agtaacttca gaaggcgcat cttttttcac cgctgttggc gcgttatcgt tgttgtcgca
    37801 ccctgccagc gccagcatta gcatgcaggc ggctacgcgt aactttttca tttttcatcc
    37861 ctgccacaat ggcccgttag caacgtcgaa taattattat gcgtgagaat ttacgttatg
    37921 taattcagta tcacgcccat acaatccgaa ttttagtgag aattgtggct cagacagcca
    37981 aatcagcctc tttcgcttgt cgcttttcac taaacatccg acaatttagc ctaaccccgg
    38041 caaaaatgga gatgcctatg tccacgacat ggtttgtagg agccgactgg ctcgccgaac
    38101 atattgatga cccggaaatt cagattatcg atgcccgcat ggcgtcgcct ggacaggaag
    38161 atcgtaacgt tgctcaggag tatctgaatg gacatattcc cggcgcagtg ttttttgata
    38221 tcgaagcgct ttctgatcac acttccccgc ttccgcacat gctgccgcgc ccggaaacgt
    38281 tcgccgtggc gatgcgtgaa ttaggcgtta accaggataa gcacctgatt gtctatgacg
    38341 aaggtaatct tttctcagcc ccacgagcat ggtggatgct gcgcaccttt ggtgtagaga
    38401 aagtgtcgat tctggggggt ggacttgcag gctggcagcg cgatgatctg ctgttagaag
    38461 aaggtgcagt agagctgccg gaaggagagt ttaacgccgc gtttaatcct gaagccgtgg
    38521 tgaaagtaac cgatgtatta ttggcaagcc atgaaaatac ggcgcaaatt attgatgccc
    38581 gcccggctgc acgttttaac gcagaagttg atgaacctcg cccaggttta cgtcgcggac
    38641 atattcccgg agcactgaat gttccgtgga cggaactggt gcgcgaaggc gaactaaaaa
    38701 cgaccgatga actggatgcg atattttttg gtcgcggcgt cagctacgac aaaccaatta
    38761 tcgtcagctg cggctctggt gtaacggcag ccgtggtttt gttagcactc gcgacgctgg
    38821 atgtgccaaa cgtgaaactg tacgacggcg catggagtga atggggcgcg cgggcagatt
    38881 taccggttga gccagtgaaa taagtatttt acaggcaata aaaaaccgcc gaatttggcg
    38941 gttttttatt gctggtctgg ttcgcggcct ttccagcagg ttgtattacc gtagtaatgc
    39001 aagcgcgtct cagcggagac aatactcgcc agtaactctc tttttgtcaa gcaaaagaga
    39061 gttattattg ttctgttagt gtattatcca ctgcggccct ttccgccgtc tcgcaagcgg
    39121 gcgctggctt aaggaaagga tgttccgcga ccgttagagc aagcgcgtct cagcgcttgc
    39181 tatcgcggca atatcgccag tggtgctgtc gtgatgcggt cttcgcatgg accgcacaat
    39241 gaagatacgg tgcttctgta tcgtacttat tgtttctggt gcgctgtttt cccaagtaaa
    39301 taataaccgg agtctctccg gcgacaattt actggtggtt aacaaccttc agagcagcaa
    39361 gtaagcccga atgccgccct ttagggcggc atattttaga ttatccggtt ttgtttaaag
    39421 tcacgcaaaa aaccacccca gcgacgttca tagaatggca caatatgttc ggtaataaag
    39481 tggctaattc ctttttcccc ttttttcacc tgacaaatat cgattggttc atcgccaggt
    39541 aatgtatcgg tcgctacact tcccgtcgcc tgaataattt cttcgatatc accatcggct
    39601 tcaatgccaa taagtaaatt aggctgtgcc tcttcgttct ctttaattga acaaataaaa
    39661 gcacgcttca ccggcttaat ggttttaaat aaggtggtga gtgaatcaat catttgtgct
    39721 ggcggctctg cgacttccga taatatcagc gattcaccgc cttccaggat ttcctggctg
    39781 ctcagcggat ttccctcttc accaatcaac aaactgattt cacgcggcat aaattcttta
    39841 ccggtcggca gtttggcatt aaggaagagc gtttcgccaa gggtcatctc aaacaatgtg
    39901 cgaacgggca ttacgacaaa tgcctgttcg tcttcaaccg cctgttgaag cgcttctaac
    39961 gaggtgaaaa aaggaatgac gctggtgccg tcttcttttt cccagtgctg taaatcaagc
    40021 gcactatctt caaccacagc ctcgccctgc gccgccgtac caggcaccca gacggtggat
    40081 tccagtagag tacggaaaaa ggccgggcgg tgcgccggtt cggttgctgc tttttccagc
    40141 aggtcttcaa gttcgttttt tgtttcggac ataagaacca caattcattc aacgttcggg
    40201 gcgcaaatgc ctgatgcgct acgcttatca ggcctacaag ggattcgcaa tttgttgaat
    40261 ttgcagaatt tgtaggccgg ataaggcgtt tacgccgcat ccggcatatt agtttacgcc
    40321 gttaacagat tagctatcgt gcgcacacca agtcccgtag cgcccgcaga ccactgttca
    40381 accggcgctt tacggtaagt cgccgagcag tcgatatgca gccagccttg ctgatagttc
    40441 tcaacaaagt gcgacaggaa gcccgccgcc gtgctcgcgc ctgccggata cgccgcgctt
    40501 ccggtattgt tcagttcggc aaagttagac ggcagctggc tgcggtggaa ttccgccagc
    40561 ggcagacgcc agaatggttc gttctcttgt gaggcactcg ccagcaaacg accggcaagc
    40621 gcatcgtcaa aactgaacag cgcgtgataa tcattaccca gcgcagtttt cgccgccccg
    40681 gtgagggtcg ccgcatcaat gatcatttcc ggtttctgcg cactggcatc aatcagacca
    40741 tcggcaagca ccagacgccc ttcggcatca gtgttcatca cttcaacttt tttaccgttg
    40801 cgataggtga tgatatcgcc cagcttgaac gcattgccgc taatcaggtt atccgcacag
    40861 cagaggaaca gcttcacgcg cttgttcagt ccgcgcgtaa tggcaaatgc cagcgccccg
    40921 gtaaccgttg ccgcgccgcc catgtccgac ttcatcgagt ccataaacgc agtctgtttg
    40981 atgctgtagc cgccggagtc aaaagtgata cctttaccta ccaggcacgc gtacactggc
    41041 gcttctttat cgccagttgg gttgtaatcc agcgccagca ataccggaga acgttctgaa
    41101 ccgcgtccga cggtgtgcag ccccatataa ccttgctcac gcagatcttc gcctttggtg
    41161 atccgataag tcacacgatc gcccgcgacg ttgctgatca gatcaacagc acgctgtgcc
    41221 agttgcgatg gtcccaattc ttctgccggt gcgttgatgg tgtcacgcac ccagtcgatg
    41281 atcatcaggc ggttatccag ttcctggcgc tgggcatcgt ccagatccgg ccactccact
    41341 ttacgcgtgc ctttcggggc tttgtaacct tgccagaatg cccagcagcg atccgcatcc
    41401 cagccttcac cgcttaactg aacatgcttg atgcccagac cgtcaatctt gcgcgccgca
    41461 cgctggatca gccccagatc gtctgccccg ttcaaatgca gggtaatgcc gtcattatta
    41521 atgctgtaag ttgctttttc tccccagcgc gcgtcggcag gttgggtaga gagggtaatc
    41581 ttcatcgctt ctgtcatttt agttatcctt ctttgtaaaa gggccgccag ttggcagccc
    41641 caggattttt ccgctaaatg attcgcgttg cagaaaggcg gcaagtgagt gaagccccag
    41701 gagcatagat aactatgtga ctggggtgaa cgagcgcagc caacgcatct gcggcgtgaa
    41761 gcatgacgcg gaaattattc ggcctcgtcc agccagacta acaaaatcgc ttcgagaatt
    41821 ttctcgttgg atgcctgcgg gtcgtcgtcg aaatcttcca gatcgcaaat ccactgatgc
    41881 atatcggtga atcgaaccgt tttcggatca agatcaggat acgcatcgta cagtgcttcg
    41941 ccaatttcgc ggctatcggt ccacttaagt cccatactaa cctctgttaa tgctcacgcg
    42001 catggttgat agtgtaacgc gggatttcga ctactaaatc ttcgtcggta acacgtgcct
    42061 ggcagcttaa acggctttcg ggctccagtc cccaggcttt gtccagcatg tcgtcttcct
    42121 gctctgagct ttctggcagc gagtcaaaac cttcacgaac gatgcagtgg caggtggtgc
    42181 aagcacagga tttttcacag gcgtgttcaa tctcgatacc gttacgcaga gctgcgtcga
    42241 gaatggtttc accgctatta gcttccagaa cagcgccatc aggacagaga tcctgatgag
    42301 gcaaaataac aatctttggc atattaaacc tcgtccacgg aatggccttt cagcgcacga
    42361 cgaaccgact ggtccatgcg gcgagcggcg aaatcctggg tttgtttgtc tacgttttta
    42421 atcgcttgtt cgatggcgtc aacgtcatcg ccctgcgcca cttcactcag gtgagcggca
    42481 gcatcgtcaa tgacctgacg ttctgcggcg cttaacagcg cggcatcagc agccagcgcg
    42541 ccgtgcagac tttccagcac acgcgccgct tctacttttt gttctgccag cattcgggct
    42601 tttacgtcct gctcggcata gctcattgag tctttgatca tcgaagcgat ttcgctatcg
    42661 gtcagaccgt aagacggttt gacctgaata gacgcctcaa cgccggtgga tttctccatc
    42721 gccgtcacgc tcaaaagacc gtcggcatcg acctggaacg tcacgcgaat atgcgcacca
    42781 ccagccggta gcgccggaat accacgcagc gcaaaacgcg ccagtgagcg gcagtcctgc
    42841 accagttcgc gctcaccctg cattacatgg atagacatcg ccgtctgacc atctttaaag
    42901 gtggtgaaat cctgagcgcg ggccaccgga atagtggtat tacgcggaat cactttctcc
    42961 accaggccgc ccatcgtttc gaggcccagc gacagtggga tcacatcaag cagcagcatt
    43021 tcgctgtctg gcttgttacc caccagaata tccgcctgaa tcgccgcgcc aatagcgacg
    43081 actttatccg ggtcgatgga agtcagcggt ggacgaccga aaaattcgcc tacccgttca
    43141 cgcaccagcg gcacgcgagt agaaccgccc accatcacca cttccagcac ttcgtcagct
    43201 tctacacccg cgtctttaag cgcgcgacga caagccagta aggttcgttt taccagtggc
    43261 gcgatcagtt cattgaattg ttcacggctg atttcgccct gccagcccgc aacgttaacg
    43321 gtcacggagt ccgcatcgct cagcgcgatt ttggctgcaa tggcggcatc cagcagttca
    43381 cgctgaacgc ggttatcgct acgatcagga atgcccgcct gctcgcgaat gtaatccgcc
    43441 agcagatggt cgaaatcatc gccgccgagc gcggaatcac cgccggttgc cagcacttca
    43501 aacacgccgc gacttaagcg cagaatggaa atatcaaacg tcccgccacc gaggtcataa
    43561 acagcgatca cgccttcctg accggaatcc agcccgtagg cgatagccgc agcggtcggt
    43621 tcgttaagta agcgcagaac atgcaacccc gccagacgcg ccgcgtcttt ggtgccctga
    43681 cgctgggcat cgtcaaagta cgccggaacg gtgataacta caccatccag ctcgcctgcc
    43741 agggcttcag ttgcccgcgc cgccagtgct ttgaggatgt ccgcagaaac gcgcaccggg
    43801 ttcagcagcc ccgccgccgt ttcaatcatc ggcaggccgt tttcgctggc ctggaattga
    43861 taaggcagat gcggatagcg ttgctggata tcagccagcg agcgccccat caggcgttta
    43921 acagaactaa tggtgttggc ggtgtcaagc gctgcgttag tacgcgcgtc ataacccacc
    43981 gaatgccctt gctgttgata gtgaacaaca gatggcagca ggtgacggcc ttcatggtcg
    44041 gctaacgttt cggcctgacc gctgcgcact gtcgccacca gcgagttggt tgtgcccagg
    44101 tcaataccag ccgccagacg acgctgatgc ggcgcagcac tcaaaccagg ttcactaatt
    44161 tgtaataagg ccatgtttag cttccagaaa ttaaaaatcg agcagttttt cttcgagttg
    44221 ttcggcactg ctccgcagtt tatcgagaaa acgcagctta cgcacggtat ccgccgccgc
    44281 gtcccacgcc tcgttgtcta actgttcaac catcaactga tggcgggtat caaacatctt
    44341 tttcacacgt ttgataaagc tttccagccg cgcttcatct ttcgcctgtt cgatctcgtc
    44401 cagctcttcg cgcagctcca actgttccat caggaacgcg gtgtcgcgca cagtatgctg
    44461 ctcgctggcg agatcaaaac cgtgtaaaga aagcaaatat tccgcgcgca ttaacggatg
    44521 acgcagcgtt tgccaggcct ggttaatggt tgcagattgc tgtacggcgg cgagttgttc
    44581 cgcctggctt ccgctggcga atttatcagg atgatactga cgttgtagat cctgaaaacg
    44641 caggctcagc gcctgggtat cgagttgata acgggcaggc aagccaaaga aggtgaagta
    44701 atccataaca ttctcagggc tgcgatccgc cgcgataacg gcgaatcagg taaaacaaac
    44761 cccacgcgca ggcgaccacg gtggggttat cggtatgcgc atcaaacgtg gaagctttcg
    44821 ccgcaaccac actcatcttt gacgttcggg ttggtgaatt taaacccttc gttcaggcct
    44881 tcttttacga agtccagctg cgtaccgtcc agaaattgca ggcttttgcc atcgaccacg
    44941 actttcacgc ctttgtcttc aaacacgatg tcttccggcg tcggttcgtc aacaaattcc
    45001 agtacataag ccatacctga acatccggag gttctcacgc ccagacgcag gccaaaccct
    45061 ttgccgcggt tagccagaaa ggtatttact cgcgctgctg cactgtcgct cagtgtaatc
    45121 gacataacca aacctcaact cttattttgc ttcacgtttg cttttatagt ccgcaatggc
    45181 ggctttgatc gcgtcttctg ccagaataga acagtgaatt ttcaccggcg gcagttcaag
    45241 ttcttcagca atatcggtat ttttgatcgc ctgcgcttcg tcgagagact tccctttcac
    45301 ccattcggtg accagggagc tggaagcgat agcagaaccg cagccgtaag ttttaaaacg
    45361 cgcgtcttca atgatacctt catcgttgac tttaatctgc aacttcatca cgtcgccaca
    45421 ggccggtgcc cccaccatgc cgctgccgac gttctcgtcg ttgttgtcaa aggaacccac
    45481 gttacgcgga ttctcgtaat ggtcgataac tttttcgctg taagccatta taaattctcc
    45541 tgattccgat accgattaat gatgagccca ttcgatgctg ttcagatcca cgccctgctt
    45601 gtacatttcc cacagcggag aaaggtcacg cagacgaccg atggatttac gaactaactc
    45661 gatggtgtag tcgatctctt cttcagtagt aaaacgacct aaagagaaac ggatagagct
    45721 atgtgccagc tcgtcgttca gccccagcgc gcgcagcacg taggacggtt cgaggcttgc
    45781 tgacgtacag gcggaacctg aagaaactgc gaggtctttc agcgccataa tcagcgactc
    45841 accttcaacg tagttgaagc tgacgttgag aatgttcggc gcaccgtgtt ccaggtcacc
    45901 gttcaggtaa acttcttcga tatctttgat gccgttccac agacggttac gcaggccgcg
    45961 cagacgttcc atctcggtcg ccatctcttc ttttgcgatg cgataggcct cgcccattcc
    46021 gacgatctgg tgaacaggca gagtgccgga acgcataccg cgctcgtgac cgccgccgtg
    46081 catttgcgct tcgatgcgta cgcgcggttt acgacgtaca tacagcgcac cgataccttt
    46141 cgggccatag attttgtgac cggagaaaga catcaggtca actttcaact ggctcaggtc
    46201 gataggcagt ttgcccacgc tctgggttgc atcaacgtga tagataatgc cacgagcacg
    46261 gcacatttcg ccgatagccg cgatatcctg caccacgccg atttcgttat ttacgtgcat
    46321 gatggaaaca aggatggtgt catcacgcat cgctgcttca agttctttca gatcgataat
    46381 gccgttacgc tgcggtgcca ggtaggtgac ttcaaaacct tcgcgctcca gctgacggca
    46441 ggtatccagt accgctttgt gttcggtttt gctggtgatg atgtgcttgc cttttttctg
    46501 ataaaagttg gctgcacctt tgatcgccag gttgtcagat tcggttgcac cagaggtaaa
    46561 gacgatttca cgcggatcag cgccgaccag atcggcaatc tgattacggg cgatatctac
    46621 cgcttcttca gcctgccagc cgaaacggtg agaacgggag gccgggttac caaaggttcc
    46681 gtccatcgtc ataaactgca tcattttctc ggcaacacgc gggtccaccg gcgtggttgc
    46741 ggagtagtcg agataaatcg gtaatttcat tgctctataa actccgtaca tcactcaatg
    46801 caaggaacca ggctaccggc tggatgtacg accgtgttta cggagcgcga tacgccccgg
    46861 cctgattcta atatcttgtt atctttaacc taactgtttc gggtttcagc aagttgacaa
    46921 acttacgaat ctccgggagc ttacataagt aagtgaccgg agtgagtatg tgcagtcaac
    46981 gcagatgcaa cacgaaacag gacggttaaa ttaagcgcgt aacttaacgt cgatcgcgtc
    47041 ttgtatgcgg gtgcgtggcg cgtcgtgagt atgctgacga ccagacacat ccagcacttc
    47101 ctggttatta accagttcgc ctaaagtaat gttgttgaga aaaccggtga gacggtcgct
    47161 caaatcacgc cacagcgcgt gggtcaggca tttatcgccg ccctggcaac cgcctttacc
    47221 ctgacaacgg gtagcatcta cagattcgtc aacggcgcta atcacttcgc caacggcgat
    47281 gctgctggca tctttgccta acagataacc accgcctggt ccacgtacgc tggaaaccag
    47341 accattttta cgcagacggg aaaacagttg ttccagataa gaaagggaaa ttccctgacg
    47401 ttcggaaata tcagccaacg gtaccgggcc cgcttcagag ttgagcgcaa cgtcaagcat
    47461 tgcggtcacg gcatagcgcc ctttagatgt cagtctcatg tcttacttca cctcaaactc
    47521 gcccctgccc ggggtttttt attgtaaaag tgggggtatt gcatagcagg gtcaagtctg
    47581 acattcccga gtaaattggt caactattta cttgactgat ttagtcgggt atttaacctt
    47641 cagtgccatt tttttatcgt ggcgtttgta cctgttgtcc ttccagacca tgggcgggtt
    47701 gagggcaatt attcgcatta gcgctggcct cttcccctca ccctaaccct ctccccgaag
    47761 gggcgagggg accgtccgtg catttatctg gcttagccct caggccttat tacctttatt
    47821 ctgctgctct atagaagcca gaatcccgcg caggatattc aattcctggc tttccgggcg
    47881 cgcacgggta aacagacggc gcaatttatt catcacctgc cccggatggt tttcacggat
    47941 aaaaccggtt gccagcagcg tttgttccag atgaccataa aaacgctcca gatcatcgac
    48001 cagcggatac ggcgtctctt catgctcgac ctgctcgcca ttttcctgag ttgccagcca
    48061 ggccatacgc acttcatagg caataacctg aaccgccatt gccaagttca gcgaactgta
    48121 ttccgggttt gccgcaatcg cgacatgata atggcatttc tgcaactctt cattggtcaa
    48181 gccgacgcgc tcacgaccaa acaccagcgc caccggggta tttgccgctt cagcgacgct
    48241 tttcagaccg cattcgcgcg ggtcgagcat cggccacggc agcgtgcggg agcgtgcgct
    48301 ggtgcccacc accaggctac aaccggctaa cgcttcgtcg agcgtatcga cgatgtgagc
    48361 attaccaatc acatcgctgg cccctgctgc cagggcaatc gcctgggagt cgggtttcac
    48421 cagtggatta accagccaca gattggttaa tcccattgtt ttcatggcac gggcaacaga
    48481 acccatattg ccggtgtgtg acgtctccac cagcacaatt cgaatatttt gcagcattgt
    48541 ctttcttcat ctaaagatta ttcacgcatc ttatcataaa acgaagacag atgccgatct
    48601 cgctgctata ctctgcgccg ttttcccgtt ctttaacatc cagtgagaga gaccgatgca
    48661 tccgatgctg aacatcgccg tgcgcgcagc gcgcaaggcg ggtaatttaa ttgccaaaaa
    48721 ctatgaaacc ccggacgctg tagaagcgag ccagaaaggc agtaacgatt tcgtgaccaa
    48781 cgtagataaa gctgccgaag cggtgattat cgacacgatt cgtaaatctt actcacagca
    48841 caccatcatc accgaagaaa gcggtgaact tgaaggtact gatcaggatg ttcaatgggt
    48901 tatcgatcca ctggatggca ctaccaactt tatcaaacgt ctgccgcact tcgcggtatc
    48961 tatcgccgtt cgtatcaaag gccgcaccga agttgctgtg gtatacgatc ctatgcgtaa
    49021 cgaactgttc accgccactc gcggtcaggg cgcacagctg aacggctacc gtctgcgcgg
    49081 tagcaccgct cgcgatctcg acggtaccat tctggcgacc ggcttcccgt tcaaagcaaa
    49141 acagtacgcc actacctaca tcaacatcgt cggcaaactg ttcaacgaat gtgcagactt
    49201 ccgtcgtacc ggttctgcgg cgctggatct ggcttacgtc gctgcgggtc gtgttgacgg
    49261 tttctttgaa atcggtctgc gtccgtggga tttcgcggca ggcgagctgc tggttcgtga
    49321 agcaggcggc atcgtcagcg acttcaccgg tggtcataac tacatgctga ccggtaacat
    49381 cgttgctggt aacccgcgcg ttgtgaaagc catgctggcg aacatgcgtg acgagttaag
    49441 cgacgctctg aagcgttaat gactcaggcg ggtgatgtca ctcacccgcc ctcgcctttc
    49501 aggcgctatt ctgaaatact tcctcaccgc tttactttct ttcgtcactc tcccaccatc
    49561 tttccccgat taatggatga taaagaagtc acaaggaaag gttatggcgc tgccagtgaa
    49621 caaacgcgtt ctcaaaattc tgtttattct ctttgttgtt gccttctgcg tttatttagt
    49681 gccgcgcgtt gccatcaact tcttctatta tcctgacgat aaaatttacg gtcccgaccc
    49741 ctggtcggcg gaatccgtcg aatttacggc taaggacggt actcgtctgc aaggctggtt
    49801 tattccttct tcgacgggcc ctgctgacaa tgccatcgca accatcattc atgctcacgg
    49861 caatgccgga aatatgtccg cccactggcc gctggtcagt tggttacccg agcgtaattt
    49921 caacgttttt atgtttgatt atcgcgggtt tggtaaatca aaaggcacgc cgtcccaggc
    49981 cggattgctg gacgatacgc aaagtgccat caatgtggtg cgccatcgca gtgatgtaaa
    50041 cccacaacgg ctggtgctgt tcgggcagag tattggcggg gcgaatattc ttgctgttat
    50101 tggtcaggat gatcgtgaag gcatacgtgc ggtgatcctc gactccacat ttgcctctta
    50161 tgcaaccatc gccaatcaga tgatccccgg cagtggctac ttacttgatg agagttacag
    50221 cggcgaaaat tatatcgcca gcgtcagccc gatcccgctt ttactcattc acggtaaagc
    50281 tgatcacgtt atcccatggc agcacagcga aaagttgtat agcctggcaa aagagacaaa
    50341 acggttgatc ctaatcccgg atggcgaaca cattgatgct ttttccgatc gtcacggcga
    50401 tgtttatcgc gaacagatgg tggactttat ccttagcgcg ttgaatccgc agaactaacc
    50461 catgatcgct agcacgataa tcattcacaa aaccacctta agacatgcta atccactggt
    50521 cagaacagtt taagatgaga aaaattctgt gacgcttgcc aacatttctg atgattagca
    50581 ttcccttcgc catttccctg agcaaacttt agctattctt atcaattatg cttatgggag
    50641 atctacagat gatgcctacg cttgctccac catctgtcct ttcggctccc cagcgccgct
    50701 gccagatctt gctgacgctc tttcagccgg ggttgaccgc caccatggca accttcagcg
    50761 agcttaatgg tgtggatgat gatattgcca gtcttgatat cagcgaaaca ggacgggaga
    50821 tcctgcgcta tcatcaactc acactgacga ctggttatga cggtagctac cgggttgaag
    50881 gtacagtgct taaccaacgt ttgtgtttat ttcactggct acgacgtggt ttccgtctgt
    50941 gtccgtcatt tattaccagc caattcaccc ccgccctgaa gagtgaactg aagcggcgcg
    51001 gaattgcgcg taacttttac gacgatacca atctacaagc gttagtgaat ctctgctccc
    51061 gacggctgca aaaacgcttt gaaacgcgcg atattcattt cctgtgtctg tatctgcaat
    51121 attgtttgct gcaacaccac gctggaatta cgcctcagtt taatccgctc caacgtcgct
    51181 gggcggaatc ctgccttgaa tttcaggtag cgcaggaaat tggacgccac tggcagcgtc
    51241 gggcgctcca gcctgtaccg cctgatgagc cactgtttat ggcattactt ttttccatgt
    51301 tgcgggttcc cgatccattg cgtgatgcgc atcagcgaga cagacaattg cgtcagtcta
    51361 tcaaacgtct ggtaaaccat tttcgtgagc tgggaaatgt tcgtttttat gatgaacagg
    51421 ggttatgcga tcagctttat acccacctcg cccaggcgtt aaatcgcagt ttgtttgcca
    51481 tcggtattga taataccctg ccggaagagt tcgccagact gtacccacgc ctggtgcgca
    51541 ccacccgcgc ggcgctggcc ggatttgaaa gtgaatacgg cgtccatctt tctgatgagg
    51601 aaagcggtct ggtcgcggtg attttcggtg cctggctaat gcaggaaaac gatctgcatg
    51661 aaaaacagat tattctactg accgggaatg atagcgagcg agaagcgcag attgagcagc
    51721 agttacgcga actaacgtta ctgccgctca acattaagca tatgtcggta aaggcatttt
    51781 tgcagacagg cgctccgcgt ggcgcggcac tgattattgc gccttatacc atgccattac
    51841 cgctcttttc accaccgctg atctatacgg acctgacgct gacaacacat caacaggagc
    51901 agatccgcaa aatgctcgaa tcagcatgag ggaacaactt tcgggcgcaa aaacattgcc
    51961 ggaagcgcca ccagcgccat tacccagaac acgccgtggc ccagatattg atacaggaaa
    52021 ccggcgaaaa cggtcatgat agcgatactg ccgcccatcg cgacggcaga gtaaaccgcc
    52081 tgtaaacgga tgacttcgct accctggcga acagcaatat agcgcatggc ggccaggtgg
    52141 cagaccgtga aggtgccgca atgcaaaatt tgcaccacta tcaaccacgg caacgccgta
    52201 gttgctccca taatgcccca gcgcactacg ccgcaaatcg ccgagatcaa cagcatatcg
    52261 cgagcactac aacggcggaa aagtttatta ctcagcgcaa agataatgac ttccgccacc
    52321 acgcccagcg accacaaata ccccaccgcc gaggccgagt agccagctgc ctgccagtaa
    52381 atggcgctaa aaccgtaata ggccgcatgt gccccctgca ataaacaaac gcaggccaga
    52441 aagcgccagt tctggcgaac cagcgccaac cacgcagacc aaccggtgct ctcctgctgg
    52501 cgacttgccc cttgtggctg aatcgtcgga cggatgagaa agccgagcag catggatgcc
    52561 acgcccaacg tcaacagcgc gaggatcacc cgataatcaa acatagtgac cagtttgccc
    52621 gtcagcgccg agccaatgac aaacgccacc gagccccaca gtcgcacttt gccgtaatca
    52681 agcgggaact gcttttgcca cgtattcgcc agtgcatcgg tcaacggtac cagcggtgag
    52741 aaaaagaggt taaagccaat catcaccagc atcagccacg ctacgtgcgc ccccgcccag
    52801 aaggcgacag caaagagaag tgtcagcagt gccagcacgc gcaaggcgga aatcaggcgg
    52861 gaaggatcgc tgacgcgggg cgcgatgagc aaactcccga gaaaacgggc aaccagacct
    52921 gcccccaata acaggccgat ggtttctggc gttaaaccaa tccctttaag ccagacgctc
    52981 cagaaaggta gaaaaatgcc gtaactaaaa aagtatgtga aatagccgag cgccaaccag
    53041 cgcgtggatt gcaaaaccat gagtccctcc cgtcaggagg cgttagtctg gggttaatcg
    53101 aatgttggtg caagttgaaa aataagaata ttcgagcctg cttcccgatg tctgtaacgc
    53161 cagttcacaa gcactaaaaa actgcgatcc gcgacgcatg ttgcaaaaat aatgcgtaat
    53221 tatgccgtta cgcttgccaa acgttcctgc acaatggcga tgaaatcgcg taatgccggc
    53281 ttcatctccc ctttcttcca cgccatcagt aaagcaatgg aaggcacatt tccggcaatg
    53341 ggacgaaaaa caacctgccc ggtattaaaa ttattcatat aaccgggtat caaagtgacg
    53401 cccagcccca tgcccaccag attcatggtc accagaatat tcgttgccac ctggacgata
    53461 tttggctggc tgttttcttg cgcaaaccac gctttaacga tcggcgcaag cgaaccggaa
    53521 tacgccggat cggtactgac gaaattcacg ccatccagtt gcgcggcggt gatctctttt
    53581 tcatgtgcta aagggtgatc aaccggtaac accaccacta atggttcgtc aaaaagctcc
    53641 agataatcaa tctccgggct ataaacagga tggcgcatta agccgacgtc gagttcacca
    53701 cggcgaattt tttcctcctg ttgcgtggtg attaaactca ccagctcaat caaggtgtct
    53761 ggctgtctga gacgaaacat tggtaatact tttggcaata aattcacttc cgccgatggc
    53821 acaaagccaa tggttaattg tctgtcttcc tgaacaattt tccgcgcccg taatttggca
    53881 ttttccgctt gttcgaggat tgccagcgcg tcctggagaa aacattctcc cgccgccgtc
    53941 aacgcgactt tgcgcttatc cctcaccagt aacggaacac cgacacagtt ttcaagatcg
    54001 cggatctggc tgcttaacga aggctgtgag gtatgcagtt tttccgccgc acgggtaaag
    54061 ttcagtgcct gcgccactgc gacgaaatag cgtaaatgcc gtagttccat caccttcccc
    54121 ttgttatcga aaaaacgtct cagctggtag aaattaacta tttcacatat tagcaaccaa
    54181 ccaacaacat ccttatgaca caaaaataga aggtcaatac atcttatctt tcaggattaa
    54241 aaaatatgac cacaccctca gatttaaaca tttaccaact gattgatacc caaaatggtc
    54301 gggtcactcc gcgtatttat accgacccag atatttacca actggagctt gaacgtattt
    54361 tcggccgttg ctggttattt ctcgcccacg aaagccagat cccaaaacct ggtgatttct
    54421 ttaacaccta catgggggaa gatgcggttg tcgtagtgcg tcagaaagac ggcagcatta
    54481 aggcgtttct caaccaatgc cgccaccggg ccatgcgtgt gagttatgca gattgcggca
    54541 acactcgcgc ctttacctgt ccgtatcacg gctggtctta tggcattaac ggcgagttga
    54601 tcgatgtacc gctggaacct cgcgcctacc cacaagggtt gtgtaaatcc cactggggac
    54661 taaacgaagt tccttgtgtg gagagttata aagggctgat ttttggcaac tgggatacca
    54721 gcgcaccggg cctgcgtgat tacctgggtg acattgcctg gtatctggat ggcatgctgg
    54781 atcgtcgcga aggcggcacc gaaattgtcg gcggcgtaca aaagtgggtg atcaactgta
    54841 actggaaatt cccggcagag cagttcgcca gtgaccagta tcatgctctg ttcagccatg
    54901 cttctgccgt tcaggtatta ggggcgaaag atgatggcag cgataagcgc ctcggtgatg
    54961 gacaaaccgc ccgcccggtg tgggaaaccg ccaaagatgc gctgcaattt ggtcaggacg
    55021 gtcacggtag cggtttcttc tttactgaaa aaccggatgc taatgtctgg gtcgatggcg
    55081 cagtttcaag ctattaccgc gaaacctatg ccgaagcaga acaacgttta ggtgaagttc
    55141 gcgccctgcg cctggcgggt cataacaata ttttccccac gctttcatgg ctcaacggca
    55201 ctgccacgct ccgcgtctgg catccgcgcg gccctgatca agttgaagtg tgggcgttct
    55261 gtattactga caaagccgcc tccgatgaag ttaaagccgc ttttgaaaac agcgccactc
    55321 gtgcttttgg tcctgctggt tttctcgagc aggatgactc ggagaactgg tgtgaaatcc
    55381 agaaattgct taaaggccac cgcgcccgca acagcaaact gtgtctggaa atggggcttg
    55441 gtcaggaaaa gcgccgcgac gacggcattc ctggcattac taactatatc ttttcagaaa
    55501 ctgccgctcg tggaatgtac caacgctggg ccgatcttct gagtagcgaa agctggcagg
    55561 aagtgctcga taaaaccgcc gcttaccagc aggaggtgat gaaatgagtg cgcaagtttc
    55621 actagagtta catcaccgca ttagccagtt tctctttcac gaagccagct tactggacga
    55681 ctggaaattt cgtgactggc tggagcagct cgacaaagag attcgttaca ccatgcgcac
    55741 cacagttaac gcgcaaacac gcgaccgccg caaaggcgtc cagccaccga caacctggat
    55801 ttttaatgac accaaagacc agctggagcg gcgaatcgcc cgtctggaaa cgggcatggc
    55861 ctgggcagaa gagccgccgt cacgcacccg tcacttaatc agcaactgcc agataagcga
    55921 aaccgacatc ccaaacgtat ttgctgtgcg ggtaaattat ctgctttatc gggcacaaaa
    55981 agagcgcgat gaaacattct atgttggaac gcgtttcgac aaagttcgcc gtctggaaga
    56041 tgacaactgg cgcttgctgg aacgggatat cgtcctggat caagcggtaa tcacttccca
    56101 taacctgagt gtactgttct gatgaatcga atttatgcgt gtcccgttgc ggatgtgccg
    56161 gagggtgagg ctctccggat cgatacctcg cccgtcatcg ccctgttcaa cgttggcggc
    56221 gagttttatg ccattaacga tcgttgcagc catggtaatg cgtcgatgtc agaagggtat
    56281 ctggaagatg acgccacggt ggagtgcccg ctacacgccg ccagtttttg cctgaaaacg
    56341 gggaaagcgt tatgcctgcc cgccaccgat ccgctcacca cttatccagt acacgttgaa
    56401 ggtggtgaca ttttcatcga cttaccggag gcgcagccat gagcgatctg cataacgagt
    56461 ccatttttat taccggcggc ggatcgggat tagggctggc gctggtcgag cgatttatcg
    56521 aagaaggcgc gcaagttgcc acgctggaac tgtcggcggc aaaagtcgcc agtctgcgtc
    56581 agcgatttgg cgaacatatt ctggcggtgg aaggtaacgt gacctgttat gccgattatc
    56641 aacgcgcggt cgatcagatc ctgactcgtt ccggcaagct ggattgtttt atcggcaatg
    56701 caggcatctg ggatcacaat gcctcactgg ttaatactcc cgcagagacg ctcgaaaccg
    56761 gcttccacga gctgtttaac gtcaacgtac tcggttacct gctgggcgca aaagcctgcg
    56821 ctccggcgtt aatcgccagt gaaggcagca tgattttcac actgtcaaat gccgccaggt
    56881 atcctggcgg cggtggcccg ctgtacaccg ccagtaaaca tgccgcaacc ggacttattc
    56941 gccaactggc ttatgaactg gcaccgaaag tgcgggtgaa tggcgtcggc ccgtgtggta
    57001 tggccagcga cctgcgcggc ccacaggcgc tcgggcaaag tgaaacctcg ataatgcagt
    57061 ctctgacgcc ggagaaaatt gccgccattt taccgctgca atttttcccg caaccggcgg
    57121 attttacggg gccgtatgtg atgttggcat cgcggcgcaa taatcgcgca ttaagcggtg
    57181 tgatgatcaa cgctgatgcg ggtttagcga ttcgcggcat tcgccacgta gcggctgggc
    57241 tggatcttta aggaagcacg atgaaagaaa aaacgatcat tattgtcggt ggcgggcaag
    57301 cggcggcaat ggctgcggcc tcgctacgcc agcaagggtt caccggtgag ctgcatctgt
    57361 tttccgatga gcgacatctt ccttatgaac gacctccact ctcgaaatcc atgttgctgg
    57421 aagattcccc gcagttacag caggtgttac ccgctaactg gtggcaggaa aacaatgttc
    57481 atctgcattc cggtgtaacc atcaaaacgc tgggtcgcga cacacgagag ttagtgttaa
    57541 ccaacggcga aagctggcac tgggatcagc tttttatagc aatcggcgcg gcagctcgac
    57601 cgctgccgtt gcttgatgca ctgggagaac gctgctttac cctacgccat gccggtgatg
    57661 ccgccagact gcgagaagtt ctgcagcccg aacggtcagt cgtgattgtc ggtgccggaa
    57721 ctattggtct ggaactggct gccagcgcca cgcagcgcag atgtaaggtg acagtgattg
    57781 aactggcggc aaccgtcatg ggccgtaatg caccaccgcc cgtgcaacgc tatcttttac
    57841 agcgccacca gcaggctggt gtgcgcattc tgctcaataa tgccattgaa catgtggtcg
    57901 atggtgaaaa agtagaactg acgctgcaaa gtggggaaac gcttcaggct gatgtggtga
    57961 tttacggtat tggtatcagc gccaacgagc aactggctcg cgaggccaac cttgatactg
    58021 ccaatggcat tgtcattgat gaggcttgcc gcacctgcga tcccgcgatc tttgccggtg
    58081 gcgatgtggc aatcactcgt cttgataatg gtgcactaca ccgctgcgaa agctgggaaa
    58141 acgccaataa ccaggcgcaa attgccgctg ccgcaatgtt agggctaccg ctaccgctac
    58201 tgccgccgcc gtggttctgg agcgatcagt acagtgataa cttacagttt attggcgata
    58261 tgcgtggcga tgactggctt tgtcgtggca acccggaaac tcagaaggcg atttggttta
    58321 atctgcaaaa cggcgtgctt atcggtgcgg tcacgctgaa tcaggggcgt gagattcgcc
    58381 caattcgcaa atggatccag agcggcaaaa cgtttgatgc gaaactgctg atagatgaga
    58441 acatcgcgct taaatcactg taaccaggat aattagcgaa tatctcaacg cctggggcgt
    58501 ggcgaggtgc aagagtgtgt attacgttta aatcacatta tcttgcaaag ggattggtta
    58561 tgaacacact a
//
LOCUS       DAJVRF010000008        57430 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJVRF010000008 DAJVRF010000000
VERSION     DAJVRF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.008"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            145..1488
                     /gene="argG"
                     /locus_tag="OQN13_000445"
     CDS             145..1488
                     /gene="argG"
                     /locus_tag="OQN13_000445"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCS1613908.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1496..3103)
                     /locus_tag="OQN13_000446"
     CDS             complement(1496..3103)
                     /locus_tag="OQN13_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCS1613909.1"
                     /translation="MLEDFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFFVLTILV
                     VKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDPDEVVKM
                     LGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQFAYKDA
                     KNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDT
                     YVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVPLSLTAD
                     SVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFD
                     ELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIH
                     YTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWY
                     SPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDSRVVDAN
                     HDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3581..3667)
                     /locus_tag="OQN13_000447"
     tRNA            complement(3581..3667)
                     /locus_tag="OQN13_000447"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3631..3633),aa:Leu,seq:gag)
     gene            complement(3682..4014)
                     /gene="secG"
                     /locus_tag="OQN13_000448"
     CDS             complement(3682..4014)
                     /gene="secG"
                     /locus_tag="OQN13_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCS1613910.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4242..5579)
                     /gene="glmM"
                     /locus_tag="OQN13_000449"
     CDS             complement(4242..5579)
                     /gene="glmM"
                     /locus_tag="OQN13_000449"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCS1613911.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5572..6420)
                     /gene="folP"
                     /locus_tag="OQN13_000450"
     CDS             complement(5572..6420)
                     /gene="folP"
                     /locus_tag="OQN13_000450"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCS1613912.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6510..8444)
                     /gene="ftsH"
                     /locus_tag="OQN13_000451"
     CDS             complement(6510..8444)
                     /gene="ftsH"
                     /locus_tag="OQN13_000451"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCS1613913.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8544..9173)
                     /gene="rlmE"
                     /locus_tag="OQN13_000452"
     CDS             complement(8544..9173)
                     /gene="rlmE"
                     /locus_tag="OQN13_000452"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCS1613914.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9299..9592
                     /gene="yhbY"
                     /locus_tag="OQN13_000453"
     CDS             9299..9592
                     /gene="yhbY"
                     /locus_tag="OQN13_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCS1613915.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9748..10224)
                     /gene="greA"
                     /locus_tag="OQN13_000454"
     CDS             complement(9748..10224)
                     /gene="greA"
                     /locus_tag="OQN13_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCS1613916.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10472..11905
                     /gene="dacB"
                     /locus_tag="OQN13_000455"
     CDS             10472..11905
                     /gene="dacB"
                     /locus_tag="OQN13_000455"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCS1613917.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(12091..13263)
                     /gene="cgtA"
                     /locus_tag="OQN13_000456"
     CDS             complement(12091..13263)
                     /gene="cgtA"
                     /locus_tag="OQN13_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCS1613918.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13279..14244)
                     /locus_tag="OQN13_000457"
     CDS             complement(13279..14244)
                     /locus_tag="OQN13_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1613919.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14371..14628)
                     /gene="rpmA"
                     /locus_tag="OQN13_000458"
     CDS             complement(14371..14628)
                     /gene="rpmA"
                     /locus_tag="OQN13_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCS1613920.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14649..14960)
                     /gene="rplU"
                     /locus_tag="OQN13_000459"
     CDS             complement(14649..14960)
                     /gene="rplU"
                     /locus_tag="OQN13_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCS1613921.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15219..16190
                     /gene="ispB"
                     /locus_tag="OQN13_000460"
     CDS             15219..16190
                     /gene="ispB"
                     /locus_tag="OQN13_000460"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCS1613922.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16419..16697
                     /gene="sfsB"
                     /locus_tag="OQN13_000461"
     CDS             16419..16697
                     /gene="sfsB"
                     /locus_tag="OQN13_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCS1613923.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16745..18004)
                     /gene="murA"
                     /locus_tag="OQN13_000462"
     CDS             complement(16745..18004)
                     /gene="murA"
                     /locus_tag="OQN13_000462"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCS1613924.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18059..18313)
                     /gene="ibaG"
                     /locus_tag="OQN13_000463"
     CDS             complement(18059..18313)
                     /gene="ibaG"
                     /locus_tag="OQN13_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCS1613925.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18473..18766)
                     /gene="mlaB"
                     /locus_tag="OQN13_000464"
     CDS             complement(18473..18766)
                     /gene="mlaB"
                     /locus_tag="OQN13_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCS1613926.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18766..19401)
                     /gene="mlaC"
                     /locus_tag="OQN13_000465"
     CDS             complement(18766..19401)
                     /gene="mlaC"
                     /locus_tag="OQN13_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCS1613927.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19420..19971)
                     /gene="mlaD"
                     /locus_tag="OQN13_000466"
     CDS             complement(19420..19971)
                     /gene="mlaD"
                     /locus_tag="OQN13_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCS1613928.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19976..20758)
                     /gene="mlaE"
                     /locus_tag="OQN13_000467"
     CDS             complement(19976..20758)
                     /gene="mlaE"
                     /locus_tag="OQN13_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCS1613929.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLANCLIKSVVFAITVTWISL
                     FNGYDTIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20766..21575)
                     /gene="mlaF"
                     /locus_tag="OQN13_000468"
     CDS             complement(20766..21575)
                     /gene="mlaF"
                     /locus_tag="OQN13_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCS1613930.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21785..22762
                     /locus_tag="OQN13_000469"
     CDS             21785..22762
                     /locus_tag="OQN13_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCS1613931.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22776..23762
                     /gene="kdsD"
                     /locus_tag="OQN13_000470"
     CDS             22776..23762
                     /gene="kdsD"
                     /locus_tag="OQN13_000470"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCS1613932.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23783..24349
                     /gene="kdsC"
                     /locus_tag="OQN13_000471"
     CDS             23783..24349
                     /gene="kdsC"
                     /locus_tag="OQN13_000471"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCS1613933.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24346..24921
                     /gene="lptC"
                     /locus_tag="OQN13_000472"
     CDS             24346..24921
                     /gene="lptC"
                     /locus_tag="OQN13_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCS1613934.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPNSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24890..25447
                     /gene="lptA"
                     /locus_tag="OQN13_000473"
     CDS             24890..25447
                     /gene="lptA"
                     /locus_tag="OQN13_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCS1613935.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25454..26179
                     /gene="lptB"
                     /locus_tag="OQN13_000474"
     CDS             25454..26179
                     /gene="lptB"
                     /locus_tag="OQN13_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCS1613936.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26227..27660
                     /gene="rpoN"
                     /locus_tag="OQN13_000475"
     CDS             26227..27660
                     /gene="rpoN"
                     /locus_tag="OQN13_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCS1613937.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27683..27970
                     /gene="hpf"
                     /locus_tag="OQN13_000476"
     CDS             27683..27970
                     /gene="hpf"
                     /locus_tag="OQN13_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCS1613938.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28088..28579
                     /gene="ptsN"
                     /locus_tag="OQN13_000477"
     CDS             28088..28579
                     /gene="ptsN"
                     /locus_tag="OQN13_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCS1613939.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28625..29479
                     /gene="rapZ"
                     /locus_tag="OQN13_000478"
     CDS             28625..29479
                     /gene="rapZ"
                     /locus_tag="OQN13_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCS1613940.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29476..29748
                     /gene="npr"
                     /locus_tag="OQN13_000479"
     CDS             29476..29748
                     /gene="npr"
                     /locus_tag="OQN13_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCS1613941.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29962..30594
                     /gene="yrbL"
                     /locus_tag="OQN13_000480"
     CDS             29962..30594
                     /gene="yrbL"
                     /locus_tag="OQN13_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCS1613942.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30591..31319)
                     /gene="mtgA"
                     /locus_tag="OQN13_000481"
     CDS             complement(30591..31319)
                     /gene="mtgA"
                     /locus_tag="OQN13_000481"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCS1613943.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFSEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31316..31969)
                     /gene="elbB"
                     /locus_tag="OQN13_000482"
     CDS             complement(31316..31969)
                     /gene="elbB"
                     /locus_tag="OQN13_000482"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCS1613944.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32199..34535)
                     /gene="arcB"
                     /locus_tag="OQN13_000483"
     CDS             complement(32199..34535)
                     /gene="arcB"
                     /locus_tag="OQN13_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCS1613945.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPVDVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34631..35560)
                     /locus_tag="OQN13_000484"
     CDS             complement(34631..35560)
                     /locus_tag="OQN13_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCS1613946.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36235..40695
                     /gene="gltB"
                     /locus_tag="OQN13_000485"
     CDS             36235..40695
                     /gene="gltB"
                     /locus_tag="OQN13_000485"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCS1613947.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40708..42126
                     /gene="gltD"
                     /locus_tag="OQN13_000486"
     CDS             40708..42126
                     /gene="gltD"
                     /locus_tag="OQN13_000486"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCS1613948.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42310..42789
                     /locus_tag="OQN13_000487"
     CDS             42310..42789
                     /locus_tag="OQN13_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCS1613949.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            42806..43111
                     /locus_tag="OQN13_000488"
     CDS             42806..43111
                     /locus_tag="OQN13_000488"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1613950.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43187..43651)
                     /locus_tag="OQN13_000489"
     CDS             complement(43187..43651)
                     /locus_tag="OQN13_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCS1613951.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43648..44523)
                     /gene="nanK"
                     /locus_tag="OQN13_000490"
     CDS             complement(43648..44523)
                     /gene="nanK"
                     /locus_tag="OQN13_000490"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCS1613952.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44520..45209)
                     /locus_tag="OQN13_000491"
     CDS             complement(44520..45209)
                     /locus_tag="OQN13_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCS1613953.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45257..46747)
                     /gene="nanT"
                     /locus_tag="OQN13_000492"
     CDS             complement(45257..46747)
                     /gene="nanT"
                     /locus_tag="OQN13_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCS1613954.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46857..47750)
                     /gene="nanA"
                     /locus_tag="OQN13_000493"
     CDS             complement(46857..47750)
                     /gene="nanA"
                     /locus_tag="OQN13_000493"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCS1613955.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47872..48663)
                     /gene="nanR"
                     /locus_tag="OQN13_000494"
     CDS             complement(47872..48663)
                     /gene="nanR"
                     /locus_tag="OQN13_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCS1613956.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49043..50365
                     /gene="dcuC"
                     /locus_tag="OQN13_000495"
     CDS             49043..50365
                     /gene="dcuC"
                     /locus_tag="OQN13_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS1613957.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(50408..50905)
                     /gene="sspB"
                     /locus_tag="OQN13_000496"
     CDS             complement(50408..50905)
                     /gene="sspB"
                     /locus_tag="OQN13_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCS1613958.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(50911..51549)
                     /gene="sspA"
                     /locus_tag="OQN13_000497"
     CDS             complement(50911..51549)
                     /gene="sspA"
                     /locus_tag="OQN13_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCS1613959.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMCLGRS"
     gene            complement(51944..52336)
                     /gene="rpsI"
                     /locus_tag="OQN13_000498"
     CDS             complement(51944..52336)
                     /gene="rpsI"
                     /locus_tag="OQN13_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCS1613960.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(52352..52780)
                     /gene="rplM"
                     /locus_tag="OQN13_000499"
     CDS             complement(52352..52780)
                     /gene="rplM"
                     /locus_tag="OQN13_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCS1613961.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(52999..54126)
                     /gene="zapE"
                     /locus_tag="OQN13_000500"
     CDS             complement(52999..54126)
                     /gene="zapE"
                     /locus_tag="OQN13_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCS1613962.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54320..54718
                     /locus_tag="OQN13_000501"
     CDS             54320..54718
                     /locus_tag="OQN13_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCS1613963.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            54872..56239
                     /gene="degQ"
                     /locus_tag="OQN13_000502"
     CDS             54872..56239
                     /gene="degQ"
                     /locus_tag="OQN13_000502"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCS1613964.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            56329..57396
                     /gene="degS"
                     /locus_tag="OQN13_000503"
     CDS             56329..57396
                     /gene="degS"
                     /locus_tag="OQN13_000503"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCS1613965.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        14263 a        15302 c        14255 g        13610 t
ORIGIN      
        1 gcgcattatt ttacaaaaaa cacactaaac tcttcctgtc tccgataaaa gatgattaaa
       61 tgaaaactca tttattttgc ataaaaattc agtgagagcg gaaatccagg ctcatcatca
      121 gttaattaag cagggtgtta ttttatgacg acgattctca agcatctccc ggtaggtcaa
      181 cgtattggta tcgctttttc cggcggtctg gacaccagtg ccgcactgct gtggatgcga
      241 caaaagggag cggttcctta tgcatatact gcaaacctgg gccagccaga cgaagaggat
      301 tatgatgcga tccctcgtcg tgccatggaa tacggcgcgg agaacgcacg tctgatcgac
      361 tgccgcaaac aactggtggc cgaaggtatt gccgctattc agtgtggcgc atttcataac
      421 accaccggcg gcctgaccta tttcaacacg acgccgctgg gccgcgccgt gactggtacc
      481 atgctggttg ctgcgatgaa agaagatggc gtgaatatct ggggtgacgg tagcacctat
      541 aaaggaaacg atatcgaacg tttctatcgt tatggtctgc tgaccaatgc tgaactgcag
      601 atttacaaac cgtggcttga tactgacttt attgatgaac tgggcggccg tcatgagatg
      661 tctgaattta tgattgcctg cggtttcgac tacaaaatgt ctgtcgaaaa agcctactcc
      721 acagactcca acatgcttgg tgcaacgcac gaagcgaagg atctggaata cctcaactcc
      781 agcgtcaaaa tcgtcaaccc aattatgggc gtgaaattct gggatgagag cgtgaagatc
      841 ccggcagaag aagtcacagt acgctttgag caaggtcatc cggtagcgct gaacggtaaa
      901 acctttagcg acgacgtaga aatgatgctg gaagctaacc gcatcggcgg tcgtcacggc
      961 ctgggcatga gcgaccagat tgaaaaccgt atcatcgaag cgaaaagccg tggtatttac
     1021 gaagctccgg ggatggcact gctacatatc gcttatgaac gtctgttgac cggtattcac
     1081 aacgaagaca ccattgagca gtatcatgca catggtcgtc agttgggccg tctgctgtac
     1141 caggggcgtt ggtttgattc ccaggcgctg atgctgcgtg actctctgca acgctgggtt
     1201 gccagccaga tcactggtga agttaccctg gagctgcgcc gtgggaacga ttattcaatc
     1261 ctgaataccg tctcagagaa cctgacctac aagccagagc gtctgacgat ggaaaaaggc
     1321 gactcggtgt tctcgccaga tgatcgtatt ggtcaattga ccatgcgtaa cctggatatc
     1381 actgataccc gagagaaact ttttggttat gccaaaactg gcctgctctc ctcctctgcc
     1441 acttcaggcg tgccgcaggt ggagaatctg gaaaacaaag gccagtaatt cgacatcaac
     1501 cctgcccttc aggggtgggg ttaccttgct tatcctctgt aaactccttt ctgagcatca
     1561 cataatcgaa aacatcatgg tttgcatcca caacccgtga gtctccttta tagtgtgcaa
     1621 tcacttcctc cagcgttttc ggttgttccg gctttgttac ccccatccac gcattaataa
     1681 ggtaattatt gtaagctgtc gaaaagatgt tgttttccgt tgtacgatcc acgccatccc
     1741 caagaacggg gctataccag ataaacatcg ggacatgata tgcctgctgg ctagcctccc
     1801 tgcctccatg aaaatagacg ttcttcttcg tagggtcacg ttccaggccg tggtcggcaa
     1861 aatacatgac cgaggcgcgg cgatctttta ataattcaaa aacctgacct agcaaactat
     1921 ctgtgtaatg aatggagttg tcatagcagg catcctgatc gtcctgcggt tgaaacacgg
     1981 cgctggattg cggataggcg ctacaagccg gttcatggct tccatttaaa tgaagaacaa
     2041 tcagtttctt ttgctgcgta ttttgctgta atgcttgcga taaatgcggc aacaacaatt
     2101 catcaaatcc tctgacataa actgtttcca tggcgcgcat ggcgatactg gtaactgctg
     2161 taccattctg ccgaaaagcg gattgcgagc ttagccagaa agtctgaaat cctgcttgat
     2221 tagccatatt aataatgttg tcggggtaat tatgaatgtc atgactcaaa acagaatcag
     2281 cagttaaaga aaggggaacc gacagcgcag tgtaaggtgc gccgcttatt gattgattaa
     2341 acagtttgat ctgttttctt tgtgcttcaa cttgcggtgt cgtagagcgt gtatatccat
     2401 acaaagacat attgtcgaca cgtacagact cccccacaat caacacgtag gtatcaatac
     2461 ctgtatccct gactgataat tgaaaatacg gcaccgtgtt tgcaattgag agtaatcttt
     2521 gatgctcttt cgctgctaaa gcaaaataat tgagattgaa aaacggcgta taggtagcaa
     2581 atcgcgacgc tagtatatat ggactgaatg cttttttatt ttttgcatct ttataagcaa
     2641 attgacaagc agaaaataaa ctgcccgaaa tgacaatcag caataatatt cctgtcactt
     2701 tttttgtcgg caaggaaaca tcatatttta ttattactgc caaaaaaaga agggataaaa
     2761 aagcaaaagc acatagataa gggatataca tccccagcat tttgacaact tcatccggat
     2821 cactctgcag cacactaatc gcaaatccat cattgaaagt tgtaccaaag gtacaccatg
     2881 aatagaggct aatactcatg tctgccgcag tcagtaacac aaatggtgcg gcaacaagca
     2941 ggcgaagcgg aagggatgat atacgtttta caaccaggat ggtcaggacg aagaacagca
     3001 atcgctgaac catatgcgcg ccggaagagg cgactaagtt cgtaatacca aaaacaataa
     3061 cacaaagata caacaaccaa tgagatttaa aatcttctag caaatttgtt gaatactgtc
     3121 atccagaaat ccaagttcac aaccaatata aacaattatc aacaatgttc aattttatat
     3181 cagacatgtt tttcaactct gatccatatc agaccgaaac actgttagtt ttttcttttt
     3241 aaatgatgcg gccattgcgt tctcagtaat taaataacat tatcaatgcg tctctaaaca
     3301 ttacgcagaa cgtagccatc agtagttata ctgacaggag caggatggtt tatcgagaag
     3361 taaacatatc cgtattcgta ccggaactgg agttgacaca ataaagtgcc aattatgtca
     3421 gcagaaggga aaagtttata actaaggcgt accggcacca tcgtttcaag gtaccagcta
     3481 cgagtaaagc aactggacga gatacagata cctgacaacc attcctcaga ccaggaccaa
     3541 aacgaaaaaa gacgcttttc agcgtctctt ttctggaatt tggtaccgag gacgggactt
     3601 gaacccgtaa gccctattgg gcactaccac ctcaaggtag cgtgtctacc aattccacca
     3661 cctcggcacg gatactactt tttagttcgg gatatcgctg gtcggcttag ccggagcagc
     3721 tggctgagtt tgttcggttt tcgccggtgc actcagattt tcccattcgc tacctttatt
     3781 ggttttgttg ctattgatgt tacccagcac cagactgatg ataaagaata acgttgccag
     3841 cagcgccgtc atgcgggtca tgaagttacc agaaccactt gaaccaaaca gcgtagcgga
     3901 agcgcctgct ccgaaggagg ctcccatatc agcgccttta ccttgctgca gcatgatcag
     3961 accaacaagg ccaattgcca caataaggaa aactaccaaa agagcttcgt acataatcaa
     4021 cctgttcctt gcggagttgc cgcgtaccaa tgcttcaacc aataaagcgg gagtttttaa
     4081 tctttcccac tgaagtgggt gtgaatacta accaaagcga atgaccttcg caagggcaat
     4141 tttatcgcat tgtatcaact gcggaaaaaa acagcaaaac cgatgtgttc gctgagaaaa
     4201 aaggcgacaa tcgccgcctt tttagccagt tatttaccgt tttaaacggc ttttactgca
     4261 tcggcgatac ggtgtgcaaa ttcagttacc tgcgcttcgt cttcaccttc taccatcacg
     4321 cgaattaacg gttcggtgcc ggatttacgc aacagcacac gtccacggct acccagcgcc
     4381 gcttcaactt cggcggtcac agctttaact gactcatgct caagcggatc gccgctacct
     4441 gcggtgtaac gcacgttaac cagaatctgc gggaacattt tcatgccgct gcaaaggtcg
     4501 tgcaggctca tatggttacg tgccatcgcc gccagcacct gcaagccagc aacgatgccg
     4561 tcaccggtag tggttttatc cagcaggatc acatgaccgg aattctctgc accgatacgc
     4621 cagcctttct cctgcatttt ttccagtacg tagcggtcac ccactttcgc gcgcgcaaat
     4681 ggaattccta actgtttcaa cgccagctca agccccatgt tactcatcaa tgtacccaca
     4741 gcaccaccac gcagctggcc ctgacgaaga ccttcacgcg cgatgatata catgatctga
     4801 tcaccatcga ctttattgcc ttcatggtca accataatca cgcgatcgcc gtcgccgtcg
     4861 aaggcaatac cgagatccgc tttttcagcc agcacacgag cctggagcgc gcgaacgtcg
     4921 gtagccccca cttcggcatt gatgtttaca ccgttcggct cacaaccgat agcgataacg
     4981 ttcgccccca gttcacgcag cacgttcggc gcgatgtgat aagtcgcacc gtttgcgcaa
     5041 tccaccacaa tcttcagttc gctgaggcta agttcgttcg ggaacgtggc tttgcaaaac
     5101 tcgatatagc gacccgcagc atcaacgata cggctggctt tacccagttc tgccgaatca
     5161 acacagctga tctctttttc catttccgct tcgatggcct cttctaccgc atccggtagt
     5221 ttggtgccgt cgatagagaa aaatttaatg ccattatcgt agaacgggtt atgcgatgca
     5281 gatatcacaa ttccggcctc tgcgcggaag gtacgcgtca gataagccac ggccggtgtt
     5341 ggcatcgggc cagtgaagag tgcggaaagg cccgctgccg ccagacccgc ttccagtgct
     5401 gactccagca tatagccaga aatacgcgtg tctttaccaa taatgatctt acgggagccg
     5461 tggcgcgcca gcactttacc cgcggcccaa cccagcttaa gcacaaaatc aggtgtgatc
     5521 ggcgcatccc ctacacgacc acgaatccca tcggtaccga aatatttacg attactcata
     5581 gcgtttgttt tcctttgcag acagagtggc ttccaccacc cgcatcgctt ctacggtttc
     5641 tttgacgtca tgaacacgaa taatgtgcgc gccttgcatt gcggcaatga ccgcacaggc
     5701 cagactaccg ctcaggcgct cggacggccc cacgttcagc agctgcccaa tcatcgattt
     5761 tcgtgacata cctaccaaca gcggcaggtt gaaatggtga aattcagcca ggcgcgccag
     5821 taatgaatag ttatgggaga gatttttacc gaaaccgaat ccggggtcga gcaacaattt
     5881 ctcttttgcg atacccgcct gctcgcaacg tgctatttgc tcaataaagt agcgattcac
     5941 ttctgcaaag acatcgtcat acttcggagc ttcctgcatg gtttttggat ttccctgcat
     6001 atgcatcaga caaaccggta aaccggtttc tgcagccgcc tccagagcgc caggttcgga
     6061 aagggagcgg atatcgttaa taatgtgagc gccaactttc gctgactcac ggatgacttc
     6121 tggtttggat gtatctaccg agatccagac ttcgaagcgt tgagcaattg cctcaaccac
     6181 aggaataaca cgttgcaact cttcttcaac gctaacttcc gccgcccctg ggcgcgtgga
     6241 ctcgccacca acgtcaatga tcgtcgcgcc agcgttgatc atcagattcg catgtttcac
     6301 cgcatctatc agcgagttat gcgtgccacc atccgaaaag gaatcaggcg tgacgttgag
     6361 gatccccatt acgtgaggat ggctaaggtc cagtgaagtg ccctgggcaa agagtttcat
     6421 ggtgttatcc ctggtatgaa ttgataagaa aaaaccccgg agcacgcccc ggggttttcg
     6481 gtacaaatac agtcatctga tgcgggaact tacttgtcgc ctaactgctc tgacatggtg
     6541 ttacccgggt tcggcgtacg cggttcatca accggacgag gagcctttgg actaccattg
     6601 tcgccagaat tgttagaagc gcctggttct tcccagcccg ctggcggacg tacatcgcga
     6661 cgtgccatca ggtcatcaat ctgcggtgcg tcgatagtct catatttcat gagagcatct
     6721 ttcatcgcat gcagaatatc catattgtcg gtcagaagct gacgcgcacg attatagtta
     6781 cgctcaatca gtgctttcac ttcctggtcg atgatacgtg cagtttcatc ggacatatgt
     6841 ttcgctttcg ctacgctacg gccgaggaac acttcacctt cttcttccgc gtacagcagt
     6901 ggacccaatt tctcagagaa gccccactga gtcaccatgt tacgcgccag gttggtcgca
     6961 actttaatat cgttggacgc accggtagaa acatgttccg gcccgtagat gatctcttct
     7021 gccagacgac caccgtacag cgtagaaatc tggctttcca gtttctgacg gctggcgctg
     7081 attgcgtcgc cctcaggcaa gaagaaagtc acacccagcg cacgaccgcg tgggataatc
     7141 gtcactttgt gcaccggatc gtgttccggc accaggcgac cgataatcgc atgacccgct
     7201 tcgtggtaag ccgtcgattc tttctgcgct tccgtcatca ccatggagcg acgttccgca
     7261 cccatcatga ttttatcttt cgctttctcg aactcaacca tcgacacaac gcgtttgttg
     7321 ccacgagcag cgaacagtgc cgcttcgttt accaggttcg ccaggtcagc accggagaaa
     7381 ccaggagtac cacgggcaat gattgccgcg tcgatatcgg gtgccaatgg tacgcgacgc
     7441 atgtgaactt tcaggatctg ctcacgaccg cgaacatctg gcaagccaac cacaacctga
     7501 cggtcgaaac ggccaggacg cagcagggcc gggtcaagaa cgtccggacg gttagtcgcg
     7561 gcgataacga tgataccttc gttaccttcg aagccatcca tctcaaccag catctggttc
     7621 agagtctgtt cacgttcatc gtgaccaccg cccagaccag cgccacgctg gcggcctacg
     7681 gcgtcgattt catcgataaa gatgatgcac ggtgccgctt tcttcgcctg ttcgaacatg
     7741 tcacgaacac gggatgcacc cacaccgacg aacatttcta cgaagtcaga accggagata
     7801 gtaaagaacg gaactttcgc ttcgcctgca atcgctttcg ccagcagcgt tttaccggta
     7861 cccggaggac cgaccatcag gacgcctttc gggatcttac cgccgagttt ctggaagcgg
     7921 ctcggctcgc gcagatattc aaccagttca gcaacttctt cttttgcttc gtcgcagccc
     7981 gcaacgtcag caaaggtcgt tttgatctga tcttccgtca gcatgcgcgc tttgctctta
     8041 ccaaacgaca tggcaccttt gccaccgccg ccctgcattt gacgcatgaa gaagatccac
     8101 acaccaatca gcaacagcat cgggaaccag gagatgaaga tagaagccag caggcttggt
     8161 tcttcaggcg gttcaccgac aaccttgacg ttcttggtca acaggttatc cagtaatttc
     8221 ggatcctgaa ccggaatgta agtggtataa cggttactat ctttcttggt aacgttgatt
     8281 tcacgtccgt tgatacgcgc ttcacgaacc tggtcgttat tgacctcttg taggaaggta
     8341 gagtaatcca ccttacggcc attagactcg ctgggcccaa agctctggaa tactgacatc
     8401 agcacaacgg caatgaccag ccagagtatt aggtttttcg ccatgtcact caagggatta
     8461 acctcttatt acaactgtgt taaaaacagc gtcaggatac tctatatcca gcatctttca
     8521 aactttcgtc tgaaatctcc cggttagggt ttacgcccgg tcgctacaat atacacttcc
     8581 cgcgaacgtg cacgagaaga gtccggctta cgaactttga ccttcgtaaa cagggagcga
     8641 atttccctta gatactcatc gaaaccttcg ccctggaaca ccttcactac aaaactgcca
     8701 cctggcgcta atacatcacg acacatttct agcgccagtt ccaccagata catggcacgg
     8761 gggatatcca ccgccggtgt tccgctcatg tttggtgcca tatcggacat gacaacctgg
     8821 actttgctgt cgccaacgcg ctccagcagt gctttcatca ccagttcatc acgaaaatcg
     8881 ccctgaagaa agtctacacc aacgatagga tccataggta aaagatcgca agcgatgatg
     8941 cggcctttgc cgccaatttg ggtgaccaca tattgtgacc aaccacccgg agcagcacca
     9001 aggtcgacaa ccgtcattcc cggtttaaag agtttgtcac tttgctgtat ttcatcaagt
     9061 ttaaaccagg cacgggaacg taaccccttt ttctgtgcct gttgaacata tttatcgcta
     9121 aagtgttcct gaagccagcg gctggagctg gcagaacgct tcttacctgt catttaactt
     9181 tcccatgggg ataactcatc gtaaccaatt gcgtaaattt ttacgcgcct atttggtgat
     9241 atatgggaga tggcggtaga atgacccatt ttcaatccca acgtaagcaa aaaatacgat
     9301 gaatctgagt actaaacaaa aacagcacct gaaaggtctg gcacatccgc tcaagccagt
     9361 tgttctgctt ggcagtaatg gtttgaccga aggggtgctg gccgagattg aacaagcgtt
     9421 agagcaccat gaactcatca aggtgaaaat cgccaccgaa gatcgcgaaa caaaaacctt
     9481 gatcgtggaa gctatcgtgc gcgaaaccgg cgcctgtaat gtacaggtca tcggtaaaac
     9541 gctggtgctt tatcgcccaa ctaaagaacg taaaatctcg ctgccacgct aagattatcc
     9601 taaagttaca cacatttgct gtgtaaaacg aggggttttc cgcaggcagg agagcaaaat
     9661 gccacgctct gttcgttgat aaaaggccgc atagcggcct ttttcctttc tttacaatac
     9721 atcaacatct tgagtattgg gtaattctta caggtattcc accttaatta cttcaaattc
     9781 tacttcgccg cccggcgttt tgatgaccac aacatcatct tcttctttgc cgatcaggcc
     9841 acgagcaata ggcgagttta cagaaatcag gttttgttta aagtcagctt cgtcatcgcc
     9901 aacgatgcga taagtctgtt cttcgtcaga atccagattc agcaccgtta cggtagcacc
     9961 aaaaataacg cgcccattgt taggcatttt ggtgacatca atcacctgcg cgtttgacag
    10021 cttggcttcg atgtctttga tacggccttc gcagaagccc tgctgttcac gagctgcgtg
    10081 gtattcggcg ttttctttca ggtcgccatg ctcacgcgct tccgcgatag cagcaatgat
    10141 ttcaggacgg cgcacagatt tcagaaaatc cagctcttcg cgtaattttt cagcgccgcg
    10201 taaggtcatc ggaatagctt gcatttgtta tacctcttga atattcctga tggggcaagt
    10261 cttcacccac cccggctgtt cggcctgccc ggcataactc ctcaccggga ccagaagcaa
    10321 aaaaataccg acccaggtac aagtcccagg tcagctacaa ttcacatttt gatagtcatt
    10381 ttaccctgaa gttcccgaag ggtcatcgtt tactttatag ggcgttgcgc cgtagtatga
    10441 cggctcgatt ccaggttgtt agcgcgagat tatgcgattt tccagattta tcatcggatt
    10501 gaccagctgt atagcgttca gtgttcaggc cgcaaatgtt gatgagtaca ttactcaact
    10561 ccccgctggt gccaaccttg ccctgatggt gcaaaaagtc ggcgcgtcgg cccccgctat
    10621 tgattaccac agtcagcaga tggcgctgcc tgccagtacc cagaaagtga ttactgcgct
    10681 ggcggcgttg attcaactcg gccccgattt tcgttttacc acgacgcttg aaaccaaagg
    10741 caatgtggaa aacggcgtac ttaagggtga cttagtggcg cgatttggtg ccgatccgac
    10801 gttaaaacgt caggatattc gcaatatggt cgcgactttg aaaaaatctg gcgtcaacca
    10861 aatcgatggc aatgtgttga tagatacctc cattttcgcc agccacgata aagcccccgg
    10921 ctggccatgg aatgacatga cacaatgctt tagcgctccg cctgccgccg ccatagttga
    10981 ccgcaactgt ttctccgtct cgctctacag tgcccaaaaa cccggtgata tggcctttat
    11041 acgcgtggca tcttattacc ccgttacgat gttcagccag gtacgcaccc tcccccgtgg
    11101 ttctgccgaa gcgcaatact gcgaactgga tgtcgtacca ggcgacctga accgctttac
    11161 gctgacggga tgcctgccac aacgttctga gccgctcccg ttggcttttg ccgtgcagga
    11221 tggagccagt tatgccggtg caattctgaa agatgagtta aaacaggcgg gtatcacctg
    11281 gagcggaaca ctgctgcgcc agactcaggt taacgaacct ggaacggtag ttgccagtaa
    11341 acagtcggcc ccgctgcacg atctgcttaa gattatgctg aaaaagtcgg ataacatgat
    11401 cgccgatacg gttttccgca tgataggcca tgcgcgcttc aatgtacctg gtacatggcg
    11461 ggccgggtcg gacgctgtgc gtcagatcct gcgccagcaa gccggtgtcg atattggaaa
    11521 caccattatt gccgatggtt cagggctttc gcggcataac ctgattgccc ccgccaccat
    11581 gatgcaggtg ctgcaataca ttgcccaaca cgacaatgaa cttaacttta tctccatgct
    11641 gccgctggcg ggctatgacg gctctttgca gtaccgtgca ggtctgcatc aggcgggcgt
    11701 ggatggaaaa gtctcagcga aaaccggttc gttgcagggg gtatataacc tggcgggatt
    11761 cattaccaca gcgagcgggc aacgaatggc gtttgtgcaa tatctttctg gctatgcagt
    11821 agaacctgcg gatcagcgta atcgccgtat tccgttagtg cgttttgaaa gccgtttgta
    11881 taaagatatt tatcagaaca attagtcaaa aagaaacccc ggcacatggc cggggcttca
    11941 gattattgac aaagtgcgct ttgtttatgc cggatgcggc gtaaacgcct tatccggcct
    12001 acaaaatcgt gcaaattcaa catattgcaa ttctcttgta ggcctgataa gcgtagcgca
    12061 tcaggctgat ttggcgttta tcatcagtga ttaacgcttg taaatgaact caacgccttc
    12121 ttcgtcgtct tcgtcccagt cgtcatccca gtcttcatca tcctcttcag caatctcttc
    12181 aagctgctgg cgatgataat cgtcccacat gaattcgact ttctctggct gtttcgcttc
    12241 ttcagcctgc acgaccgggt tttcaaggat aaaggtcatc acatcccagc agagatcttt
    12301 cacgcccagt ccgctcgccg cagagatcag ataatattta tcttcccagc ccagcgcttc
    12361 agcaatcgct ttcgctttct cttcagcttc tgccttatcc agcagatcga tcttattgaa
    12421 cactaaccaa cgcggtttcg ccgccagatc ctggctgtat ttttccagct cgctgataat
    12481 aatacgcgcg ttttcaaccg gatcggtgcc gtcaatcgga tcgatatcga tgaggtgcaa
    12541 caggacgcgg caacgttcca ggtgcttcag gaagcggata cccagacctg cgccttccgc
    12601 agcgccttca atcagtcctg gaatatcggc aacaacgaag ctcttttcgt tgtccattcg
    12661 taccacaccc agacttggca ccagagtggt aaacggataa tccgccactt tcggtttagc
    12721 cgccgatacc gcgcgaataa aggtcgattt acccgcgttt ggcatcccca acataccgac
    12781 gtcagccagc agcatcagct ccagcagcag ctcacgctta tcacccggcg taccgttggt
    12841 tttctgccgt ggtgtacggt taacggacga tttgaaacgg gtattgccca gaccgtgcca
    12901 gccgccctta gcaaccagca gacgctgacc gtgtttagtc atatcgccca tggtttcacc
    12961 agtaccctgg tcgattacac gcgtacctac cggcacttta atcgtcacgt ctttaccgcg
    13021 cttaccggta caatcgcggc ttgcgccgtt ctgaccgcgc tctgcacgga aagatttttc
    13081 aaaacgataa tcgataagcg tgttcaggtt ttcgtcggct tccatccata cgtcaccacc
    13141 atcaccaccg tcgccgccat ccgggccgcc tttcggaata tacttttcgc ggcggaagct
    13201 cacgcaacca ttaccgccat cacctgcaac gaccagaatc gatgcttcat caacaaactt
    13261 cattttattc tccataaatc attcgcctgc gcggggttgc gataccaccg ttgtatgctt
    13321 acgtaatccg ccccaaatac gatgaccaat ggcggaatac atcgcgcccg caaccacgac
    13381 aaacgcaccg agataaccta aaaggtttaa catcggtctg gcgaagaaat cgggccaggc
    13441 cagtgataaa agatctgaaa aaaacagcgt aaacagtggg gtgagcgtga tgatcgcgct
    13501 cacctgcgct gcctgccagc gagccatcgc ttccgccagg gcgccatatc ctaccaatgt
    13561 attcagtccg caaaaaatta aacatgcgag ctgccagtgg ctaagctgcg ctatcactcc
    13621 aggctttgcc agagggaaga gcgcaattgt acataaagtg tacagtaaaa acaggatctg
    13681 cggtgaggcc agccgacgca ataaaacctt ttgcgccacg ccataactca cccaaaccgt
    13741 cgccgcaccg accccaaaga taactcccca ggtgtaatcg gtgagctttg taaatatctc
    13801 gaccagactg gtgttaaaaa acatcaccag gccgctcagg agcatcaatg cccctacaac
    13861 ctgagtgctg cgcattttct ctttcaggat aaatacgctg gcaaccatca tgccaactgg
    13921 cgagagttgc ccaatcacct gcgaagcggt cggactcagg tattgcaagg atgagctgaa
    13981 caggatgaag ttcccaaaca gcccggcggt cgccactgcc aacaaaatca accagcgtgg
    14041 cttacgaaac acgcgtaatg gcggcaacct cttcttcacc gcaagaatgg caccaaggcc
    14101 aatactcgcc atcaagaaac ggtaaaacac gattgtcgga ggttccatca cctccagcac
    14161 ctgcttcatt gcgattggca acgcccccca gcaaattgct gtggtgagcg ccaaaagaat
    14221 gccaatgcct gcctgctgct tcataccgta ttccctgcaa agcgcatttg cggattaacg
    14281 tcgcaccatg cgcattttta ccggttatcg aatgtaaaaa gccccgcaac gtgttgcggg
    14341 gctttcatcc gttaccggga cgcgaaaaac ttattcagct tcgatgctga taaatttacg
    14401 gtttttcggg cctttaactt cgaatttcac tttaccgtct gctttagcaa acagagtgtg
    14461 gtcacgaccg caacctacgt tagcgccagc gtggaatttg gtaccacgtt gacgaacgat
    14521 gatgctaccc gccagaacgg attcgccacc gaaacgctta acgcccaggc gtttagcttc
    14581 tgaatcgcga ccgttacgtg tggagccgcc agccttttta tgtgccattt gaaatctctc
    14641 ctcaggtctt aggcgctgat gccagtaatt ttcacatcag tgaaccactg acgatgaccc
    14701 tgctgcttac gatagtgttt acgacgacga aacttaacga ttttaacttt ctcgccacga
    14761 ccgtgagcaa caacttcagc tttgattacg ccgccatcaa cgaaaggaac gccgattttg
    14821 acttcttcac cgtttgcgat catcagcact tcagcgaact caacagtttc gccagttgcg
    14881 atgtccagct tttccaggcg aacggtctga ccttcgctta ctcggtgttg tttaccacca
    14941 ctttggaaaa ccgcgtacat ataaaactcc gcttccgcgc acaccttttc aatgattcag
    15001 agtgcgctat aaatattcac aatagggcgc gaatattacg caaaacgcac gcctttgaca
    15061 agtgctacag tcaatacacg aagaaaaaaa acacaacttg tacggtaacg tttatctgtg
    15121 ccattttttc agtacaatca ccctatattc ctaaccataa accctaagtt gcctttgttc
    15181 acagtaaggt aatcggggcg aaaagcccgg cttttgcgat gaatttagaa aaaatcaatg
    15241 agttaaccgc gcaagatatg gcgggtgtta atgcggcaat ccttgagcag cttaattccg
    15301 acgtccaact gatcaatcag ttaggctatt acatcgtcag cggcggcggt aaacgtattc
    15361 gtccgatgat tgctgtactg gctgcacgag ctgttggcta tgagggaaat gcgcatgtca
    15421 ccattgctgc cctgatcgag tttatccaca cggcgactct gctacacgac gacgttgtgg
    15481 atgaatcaga tatgcgcagg ggtaaagcta ccgccaacgc cgcatttggc aatgccgcca
    15541 gcgtgctggt aggcgatttt atttataccc gcgcgttcca gatgatgacc agcctcggtt
    15601 cactcaaagt gctggaagtc atgtcagaag ctgtaaacgt catcgcagaa ggtgaagttc
    15661 tgcaactgat gaacgttaac gatccggaca tcactgaaga aaactacatg cgcgttatct
    15721 atagcaaaac cgcgcgtctg tttgaggctg ccgcgcagtg ttccgggatt ctggctggct
    15781 gtacgccgga ggaggagaaa ggcctgcagg attatgggcg ctatctcggc actgctttcc
    15841 agttgatcga cgatttactc gattacaatg ccgatggcga acagttaggt aaaaatgtcg
    15901 gcgacgatct gaacgaaggt aaaccgacgc tgccgctgct gcatgcgatg catcatggca
    15961 caccagaaca ggcacagatg atccgtaccg ccatcgaaca gggtaacggt cgccatcttc
    16021 tggaaccggt tctggaagca atgaacgctt gtggatctct tgaatggacg cgtcagcgtg
    16081 ccgaggaaga agcagacaaa gccatcgcag cattacaggt gctcccggac accccttggc
    16141 gagaagcact catcggcctc gcgcacatcg ctgttcaacg cgatcgttaa tcccctccct
    16201 tcatcccgcg cagtgcgcgg gatgattcca gtaaattcca taaaaacact aattcagcgc
    16261 tataacacct gaaaaaaaag ggcgatgaat ctttatatat tctgaatatt cacactcttt
    16321 acaggaactt tttagagcaa taggccatca ggagtatagt gatgcccgac agaagaagtg
    16381 ttctgaatga aagcgactaa cttaaggagt gaggaaagat ggaaagtaat ttcattgact
    16441 ggcatcccgc tgacatcatt gcgggtttgc gcaagaaggg aacgtcaatg gcggcggagt
    16501 ctcgcagaaa tggtttgagt tcctcaacgc tggcaaacgc attatcgcgc ccatggccga
    16561 aaggagagat gattattgcg aaagccctgg gaactgaccc ctgggttatc tggccatcac
    16621 gctaccatga tccgcagacc catgagttta tcgacagaac gcagttgatg cgtagctaca
    16681 ctaaaccgaa aaaatgagtg gtctggcggt agccccgcga acggggctgc cagctctcag
    16741 acgattactc gcctttcaca cgctcaatat ttgcacctaa agcgcgcagt ttgtcttcaa
    16801 tgcgttcgta gccacgatcg atgtgataaa tacgatcaac caccgtcgtc ccttccgcaa
    16861 tacagccagc cagcaccagg cttgctgatg cacgcagatc ggttgccata acctgtgcgc
    16921 cagaaagttt ttcaacaccg tgacaaataa cggtattgct ttcgatttcg gcatgcgcgc
    16981 ccatacggct aagctccggc acatgcataa agcggttttc aaagaccgtt tcggtgatga
    17041 atccggtccc ttctgccacc aggttcaaca gcgtgaactg ggcctgcata tcggtcggga
    17101 atgccggatg cggcgcggta cgtacgttaa cagccttcgg acgtttgcca tgcatatcca
    17161 ggctaatcca gtcttcgccg acttcgatgt ccgctccagc gtcacgcagt ttcgccagca
    17221 cggcgtcgag agtatctggc tgcgcgttac ggcagataat tttgccgcga gaaatcgccg
    17281 ccgccaccag gaaagtaccg gtttcgatac gatccggtag cacgcgatag acaccgccgc
    17341 ctaaacgttc cacaccttcg atgacgatac gatcggtgcc ctgaccgcta attttcgcac
    17401 ccagcgtaat caggaagttc gcggtatcga cgatttccgg ttcacgcgct gcattttcaa
    17461 taatcgtggt accttccgcc agggttgcag cacacatgat ggtcaccgtt gcgccaacgc
    17521 tgactttatc catcacgata tgtgcgcctt tcagacgacc atcgacggaa gctttaacgt
    17581 aaccttcttc cagtttgatg gtcgcgccta attgttcgag gccagaaatg tgtagatcaa
    17641 ccggacgcgc accgatcgta caaccgccag gcagtgaaac ttgcccctga ccaaagcgcg
    17701 ctaccagcgg ccccagtgcc cagatagaag cacgcatggt tttaaccaga tcgtaaggtg
    17761 cgcagaatac attaacgtcg cgggcatcaa tatgcacaga accattacgt tctactttcg
    17821 cacccagctg gcttagcagc ttcattgatg tatcgacgtc tttcagtttt gggacgttct
    17881 ggatctctac cggttcttcc gccagtagag cggcaaaaag gataggcaga gcagcatttt
    17941 tagcgccgga aattgtgact tcgccctgga gcttcgttgg cccctgaaca cgaaatttat
    18001 ccatttagtt tgttctcagt taacaattca tatccgctac cggcgaatcg cccatagctc
    18061 aaaagccgtt cagtttgcga tcgcgcgccc actccgcagg ggtatacgct ttgatcgaca
    18121 cagcatgaat gcggttatcc gcaatatatt ccatcagcgg gccatagacc gtctgctgtt
    18181 ttttaacccg actcatgccg tcaaacaact cacccacggc aataacctga aagtggctgc
    18241 catcgccgga aacgtggact tcctggaggg agagagcgtt catcaacacg ctctgaattt
    18301 cattattttc catgggatct tcaatcatca gttaataaac cagcgaaaca tcttagagca
    18361 aagttgcgct ggcataaata agcaaaaagc ctcgctgata aatcagacaa ggctcgactt
    18421 gcaggcaggt ttgccggaca ggcggttaac gccatatccg gcctgaaaaa atttaacgag
    18481 gcagaacatc agcaggcaaa ttatacaatt tcgcgagggt atacactttg tcgtttaccc
    18541 cctgaagcgt cacgttgttg ccctgctttt tcgccagatc gataagatgg agcagcagtg
    18601 ccagtccacc cgtatccacg cgggacacac ggctaagatc gatgcaggta atccccttca
    18661 ccgcttcctc acgcatttcc caaagcggta gcagaacgtc ctgatccagc tctccggata
    18721 acgccagcgt gtcacccgtc tgcatccagc tcagtgactc gctcattatt ttttctcttc
    18781 cagagtgatt ttctgttgag aaatcgattt cagttgcgca gtcaggccgt cgataccttt
    18841 ggtacgcagc agcgttcccc actcgttttg tttggtggtg atcatactga cgccttcagc
    18901 aatcatgtcg taagcctgcc agtttcccgt ctgggagttt ttacgccact ggaagtccag
    18961 acgcaccggc ggacggccat tcgggtcaat aatggtaacg cgaataggca caatggtttt
    19021 atcgcccagc ggctgttctg gcgcaatctg ataggtttga ccgtgataca tcgccagcgc
    19081 ctgaccgtaa gcctgcttca ggtactcacg gaaagcggca aagtaggctt cacgttgagc
    19141 aggggtcgca ctcttgtaat actggcccag caccagcgca ccggcgtatt tcacctgtac
    19201 gtatggcagc agttcctgat caacaatggt acgcagataa tccgggttgg cccgaatttg
    19261 cggttgctca ttcttcaggc gatcgaacgt tttctgcgcc gcctcgtcca tcagcttata
    19321 aggattggtc tggtctgccg cggttgccgc actcagaggt gcaatcacca gcaaagcgac
    19381 catcattaaa cgtttaaaca tgcgtcggtt ctcctgaaat tatttcgttg tacccacagg
    19441 ttcagtggtt tcattattac ctggcgcagc agctggcgca tcgccactat tcttattgtc
    19501 atcgccttta ctaccgtaaa ggaactgacc aatgagatct tccagcacca tcgcagactt
    19561 agtgtcctga attgtatcgc catccttcag gatagcagtc cccagttccg ggtcttcaaa
    19621 accgacgttt aatgccagat attgttcccc cagcaggccg gaagtacgaa tgctcagcga
    19681 actggtatct ggaatgtggt tataacgttg ttcaatttcc agcgttacgc gcggcagata
    19741 ggttttcggg tccagcgtaa tatccgccac ccgacccaca acaacgccac caatactgac
    19801 cggagagcgc gctttcaggc cgccaatgtt atcgaacgtc gcataaagcg tgtaggtcgg
    19861 ttcagtacgt atggacgtca cgttcgccgc cttcaggcaa acaaacagcg ccgccagcag
    19921 tgctgctaat aaaaagatac ccacccaaat ttcatttttt ttcgtttgca tgaactcaat
    19981 tcccaaacat caatgcggtc agcacaaaat ccagccccag aacagccaga gacgagtgga
    20041 caacggtgcg agtggttgcc cggctaatcc cggcagacgt cgggatggtg tcgtagccgt
    20101 taaacaacga aatccacgtc accgtgatgg cgaacaccac gctcttaatc agacagttgg
    20161 ccagatccat acgccagtcg acggcatttt gcattgccga ccagaagaac ccgctatcaa
    20221 tgcctttcca actgacgccg accagagatc cgccccagat ccccacggcg acgaaaataa
    20281 ccgtcaacag tggtaatgaa ataaccccag cccagaaacg gggagaaata acccgacgca
    20341 gcggatccac cgccatcatc tccatactgg agagttgctc tgtagcgcgc atcaggccga
    20401 tttctgcggt tagcgccgaa ccagcacgcc cggcaaacaa caacgcggca accaccggcc
    20461 ccagttcacg cagtagcgat aacgccacca gcatacccag actggtttcc gcactataag
    20521 tggtcagaac cagataacct tgcagcccca acaccattcc gatgaacacg ccagaaacca
    20581 caataatcag catcgacagg acgccgacat tatagagctg gcgcaccagc agcggcgcat
    20641 gtttgcgaaa ttccggtttg ccgaccagcg cattgaataa cattaacccg gcccgcccga
    20701 acgttctcag ggttttaatc cctttatgtc cgagcgacgc cagcgcattt aacagcatga
    20761 gtggcttaac tccctggtaa aagatcagcg tgataatcgc cggcaggata gcggaacgga
    20821 acaggcccgt cagctatccc gtccagaaac tgacgtacgc gcggatcagg attcgcctgc
    20881 aacgcctggg cactgccatg agcgacaatt tttttgtccg ccaggatcca ggcgtgatcc
    20941 gcaatactta acacttccgg cacatcgtga gaaaccacca cacaagtcac gcccagcgcg
    21001 ctgttcagct cagaaatcag cttcaccagt acgcccatgg tgatgggatc ttgcccaaca
    21061 aaaggttcat caaacatgat gagatccggc tccagcgcaa tcgcacgcgc cagcgctgca
    21121 cgccgcgcca tcccaccgga aagttcagaa ggcattagtt tagccgctcc acgcagcccc
    21181 acggcctcca gcttcatcat caccgtacta tgcaacaatg gcgcgggtag ttgggtatgt
    21241 tcgcgcagtg gataggcgac gttgtcaaat acgttcatat cagtgaacaa cgccccggac
    21301 tgaaataaca tgctcatccg tttgcgcact gtatacaggc gcgaacgaga catcgccgga
    21361 atattctcac catcgaaaag gatctcacca tgatctggtg cgatttgccc gccaatcaga
    21421 cggagtagcg tcgttttacc gatgcccgat ggccccatga tcgccgtgat cttccctcgc
    21481 ggcacggtca gggaaatatt atcgaagatg cagcgattgc cacgcgtaaa actgacatcg
    21541 cgcatatcga ctaaattcgc cacagactgc tccataattc acccttcgtc ttgcgttgat
    21601 tttctaagca tggcgctcaa tttaaccttg aacccaacat atttacagaa tattacccgc
    21661 cgtggttagc gaaagctggc atttgtttta ctttttagcc gcataaagtc aaaattaaga
    21721 atccgttacg gctttctgaa aatcttcagc ggaccgacga gtatacctga agaaaggacg
    21781 ttagatgctt ttagctacgg cactgttaat tgttggttta cttttggtcg tttacagtgc
    21841 cgaccgcctg gtttttgccg cgtctattct ttgccgaacc tttggcatcc cgccgctgat
    21901 catcggcatg acggtggtca gtattggtac atcgttacca gaaatcatcg tctcgcttgc
    21961 cgcgtctctg cacgaacaac gcgatttagc cgttggtaca gccctcggct caaacattat
    22021 caatattttg ctgatcctcg gtctggctgc gctggttcgt ccttttaccg tccattccga
    22081 tgttctgcgt cgtgaattac ccttaatgtt gttggtcagc gtagtggccg gttccgtact
    22141 ctatgacgga caacttagtc gcagcgatgg tatctttctc ctctttctgg ctgtgctatg
    22201 gctgctgttc attgttaaac ttgcacgtca ggctgaacgt caggggactg acagcctgac
    22261 cagagagcag cttgcagagc tgccgcgtga cggcggattg cccgtcgcgt ttttatggct
    22321 cggcattgcg cttatcatca tgccagtggc cacgcggatg gtggttgata acgccacggt
    22381 gctggcgaat tactttgcca tcagcgagtt gacgatgggt ctgacggcaa ttgctatcgg
    22441 aaccagcctg ccggaactgg caaccgcaat agcgggggtt cgcaaaggtg aaaacgacat
    22501 tgctgtcgga aatatcattg gcgcaaacat ttttaatatt gtcatcgtgt tgggtttacc
    22561 cgcgctgata acgccaggag agattgagcc attggcgtac agtcgtgact acagcgtgat
    22621 gttgctggtg agcattattt ttgcgttgct gtgctggcgg cgctccccgc aaccgggccg
    22681 tggtgtaggg gtattattaa ctggcggatt tatcgtatgg ctggcgatgt tgtactggtt
    22741 atcgccaata ctcgttgaat aactggaaac gcattatgtc gcacgtagag ttacaaccgg
    22801 gttttgactt tcagcaagca ggtaaagaag tcctggcgat tgaacgtgaa tgcctggcgg
    22861 agcttgatca atacatcaat cagaatttca cgcttgcctg tgaaaagatg ttctggtgta
    22921 aagggaaagt tgtcgtcatg gggatgggga aatcggggca catcgggcgc aaaatggccg
    22981 caacgtttgc cagcaccggt acaccttcat ttttcgtcca tcctggtgaa gccgcgcatg
    23041 gtgatttagg catggtcacc ccacaggatg tggtgattgc tatctctaac tcaggtgaat
    23101 ccagcgaaat cacggcctta attccagtgc ttaagcgtct tcacgtaccg ttaatctgca
    23161 tcaccggtcg cccggagagc agcatggcgc gcgccgcaga tgtgcatctg tgtgttaaag
    23221 tagcgaaaga agcctgtccg ttagggctgg caccgaccag cagcaccacc gccacgctgg
    23281 ttatgggcga tgccctcgct gtcgcgctgt taaaagcacg cggctttact gctgaagatt
    23341 ttgcgctctc acacccaggc ggcgcactgg gtcgtaaact tctgctgcgc gtaaacgata
    23401 ttatgcatac gggcgatgag atcccgcatg ttaagaaaac ggccagtctg cgtgacgcgt
    23461 tgctggaagt tacccgcaaa aatcttggta tgactgtcat ttgcgatgac aatatgatga
    23521 ttgaaggcat ctttaccgac ggtgatttac gccgtgtctt cgatatgggc gtggatgttc
    23581 gtcagttaag tattgccgat gtgatgacgc cggggggaat acgtgtgcgc cctggcattc
    23641 tggccgttga ggcactgaac ttaatgcagt cccgccatat cacctccgtg atggttgccg
    23701 atggcgacca tttactcgga gtgttacata tgcatgattt actgcgtgca ggcgtagtgt
    23761 aaagattcaa ggataaacaa caatgagcaa agcaggtgcg tcgcttgcga cctgttacgg
    23821 ccctgtcagc gccgacgtta tagcaaaagc agagaacatt cgtctgctga tcctcgatgt
    23881 cgatggcgta ctgtcagatg gcctgattta tatgggcaat aatggcgaag agctgaaagc
    23941 gttcaatgtt cgtgacggtt atggcattcg ttgtgcgctc acctctgata ttgaagtcgc
    24001 tatcattacc gggcgaaagg ctaaactggt agaagatcgt tgtgccacat tggggatcac
    24061 tcacttgtat caggggcagt caaacaaact gatcgccttt agcgatctgc tggaaaaact
    24121 ggcgattgcc ccggaaaatg tggcttatgt cggcgatgat ctcatcgact ggccggtaat
    24181 ggaaaaagtg ggtttaagcg tcgccgtggc cgatgcgcat ccactgttga tcccgcgcgc
    24241 cgattacgtg acgcgcattg ctggcggtcg tggcgcagtg cgcgaagttt gcgacttatt
    24301 actcctggcg cagggcaaac tggatgaagc caaagggcaa tcgatatgag taaagccaga
    24361 cgttgggtta tcattgtgct atcactggcg gttctggtga tgatcggcat tgatatggcc
    24421 gaaaaagacg ataccgccca ggtggtcgtc aacaacaatg atcccaccta taaaagcgag
    24481 catacggaca cgctcgtcta taacccagaa ggggcactaa gctatcgatt gattgctcaa
    24541 cacgttgaat attattccga tcaggccgtt tcgtggttta cgcagccggt acttaccacg
    24601 tttgataagg ataaaatccc gacatggtcc gtaaaagcag ataaagccaa gctgaccaat
    24661 gaccggatgc tctatttata tggacacgtt gaagtcaacg cacttgtgcc aaactctcaa
    24721 cttcgcagaa tcacgacgga taacgcgcag atcaatctgg tgacgcagga tgttacctct
    24781 gaagacctcg tcacgttata cggaacaaca tttaactcca gcggtctgaa aatgcgcggc
    24841 aacttacgca gcaagaacgc cgagctgatt gaaaaggtta gaacatccta tgaaattcaa
    24901 aacaaacaaa ctcagcctta atcttgtgct tgccagctca cttctggccg ccagcattcc
    24961 ggcatttgcc gtaaccggag acactgatca gccgatccac attgaatcgg accagcaatc
    25021 tcttgatatg caaggcaacg tggttacctt taccggtaat gtcatcgtca cccagggcac
    25081 catcaaaatt aatgccgaca aagtggtcgt tacccgtccg ggcggcgaac aaggtaaaga
    25141 agtgattgac ggctacggta aaccggcaac gttctaccag atgcaggaca acggtaaacc
    25201 cgttgaaggt cacgcttccc agatgcacta cgaactggca aaagattttg tcgttctgac
    25261 gggtaatgct tatctgcagc aggtcgatag caacattaag ggcgataaga tcacttacct
    25321 ggtaaaagag cagaaaatgc aggctttcag cgacaaaggc aagcgcgtaa ccaccgttct
    25381 ggtgccgtcg cagctgcagg acaaaaacaa caaaggccag accccggcac agaagaaggg
    25441 taattaattc gttatggcaa cattaactgc aaagaacctt gcaaaagcct ataaaggccg
    25501 tcgcgtggta gaagacgtca gcctgaccgt caactccggg gaaattgtcg gtctgctggg
    25561 gccaaacggt gccggtaaga ccaccacttt ctacatggtt gtaggcattg tgccgcgcga
    25621 tgcgggcaac atcattattg atgatgacga tatcagtctg ctgcctctgc atgcacgcgc
    25681 gcgccgcggt atcggctatc tgccacagga agcctccatt ttccgtcgcc tcagcgttta
    25741 cgataacctg atggcggtac tgcaaattcg tgacgacttg tctgctgaac aacgtgaaga
    25801 ccgcgcgaac gagctgatgg aagagtttca cattgagcac ctgcgtgaca gcatggggca
    25861 gtcactctcc gggggtgaac gtcgccgtgt agaaattgcc cgcgcactgg ctgcgaatcc
    25921 gaaatttatt ctgctcgacg aaccgtttgc cggggttgac ccgatctcgg ttatcgacat
    25981 taaacgcatc attgagcacc tgcgcgacag cggcctgggc gtgctgatca ctgaccacaa
    26041 cgtgcgtgaa acactggcgg tttgtgaacg cgcttatatc gtcagtcagg ggcatttgat
    26101 cgcccacggc acgcctacag aaatcttaca agacgaacac gttaagcgtg tataccttgg
    26161 ggaagacttc agactctgat agggtagaag tttgcgacgt tttagcagga gagtacgatt
    26221 ctgaacatga agcaaggttt gcaactcagg cttagccaac aactggcgat gacgccacag
    26281 ctccaacagg caattcgtct gttgcagttg tcgacgctgg aacttcagca ggagctacag
    26341 caggcgctgg agagtaatcc gctgcttgag caaatcgaca ctcatgaaga aatcgacacc
    26401 cgcgaaacgc aagacagtga aacgctggac accgccgacg cgctcgaaca aaaagagatg
    26461 ccggaagagc tgccgctcga tgccagttgg gacaccattt acaccgctgg tacaccatcc
    26521 ggcaccagcg gtgactacat tgacgatgag ctgccggtct atcagggcga aacgacgcag
    26581 accttgcagg attacctgat gtggcaggtc gagctgacac cgttttccga cactgaccgc
    26641 gctattgcta cctctatcgt cgatgccgtt gatgacatcg gttatctgac tgtcccgctg
    26701 gaagatattc tcgaaagtat gggcgatgaa gagatcgaca tcgacgaggt tgaagccgtc
    26761 cttaagcgga tccaacggtt tgatccggtc ggtgtagcgg caaaagatct gcgtgactgc
    26821 ctgctaatcc aactctccca attcgataaa accacgccgt ggctggaaga ggccagactg
    26881 atcattagcg atcatctcga tctgttagcc aatcacgact tccgcacttt aatgcgcgtc
    26941 acgcgtctga aagaagatgt gctgaaagaa gccgtcaatc tgatccagtc gctcgatccg
    27001 cgccccgggc agtcgatcca gactggcgaa cctgagtatg tcattccaga tgtgctggtg
    27061 cgtaagcata acggtcactg gacggtagaa ctcaacagtg acagcattcc gcgtctgcaa
    27121 atcaaccagc actacgcctc gatgtgcaat aacgcgcgca acgatggtga cagccagttt
    27181 atccgcagca atctgcagga tgccaaatgg ttgatcaaga gtctggaaag ccgtaacgat
    27241 acgctactgc gcgtgagtcg ctgtatcgtt gaacagcagc aagccttctt tgagcaaggt
    27301 gaagaatata tgaaaccgat ggtactggcc gatatcgccc aggctgtcga aatgcatgaa
    27361 tcgacgatat ctcgcgtgac cacgcaaaaa tacctgcata gtccacgagg catttttgaa
    27421 ctgaagtatt tcttttccag tcacatcaat accgagggcg gcggcgaagc ttcctccacg
    27481 gcgattcgtg cgctggtgaa gaaattaatc gcggcggaaa acccagcgaa accgttgagc
    27541 gacagcaagt taacctcttt gctgtcggaa caaggtatca tggtggcacg ccgcactgtt
    27601 gcgaagtacc gagagtcttt atccattccg ccgtcaaacc agcgtaaaca gctcgtttga
    27661 cccaaccgat aaggaagaca ctatgcagct caacattacc ggaaataacg tcgagatcac
    27721 cgaggcactg cgcgaatttg ttacagccaa atttgccaaa cttgagcaat attttgaccg
    27781 aatcaaccag gtctatgttg ttctgaaagt ggagaaagtc acccacacct cagatgcaac
    27841 actgcatgta aacggtggtg aaattcatgc cagcgcggaa ggtcaggaca tgtacgctgc
    27901 cattgatggc ttaattgata agctggcacg ccagttgacc aaacataaag ataaactgaa
    27961 acaacactaa ttgtccgggc aattagcatg tgcatggcgg cctgttgtgc ggcacaacgg
    28021 gccatttgta cggttaatgc tccgagcctg ttccactgtt tgagtgggca ggttcttagg
    28081 tgaaattatg acaaataatg atacaactct acagcttagc agtgttctta acagggaatg
    28141 tacgcgaagc cgcgtccact gtcagagcaa aaaacgcgcc ctggaaatca tcagcgagct
    28201 ggcggcgaaa caacttagcc tgccgcctca ggtggttttt gaagctatcc tgacgcggga
    28261 aaaaatgggc agtaccggta tcggcaatgg tattgccatt ccgcatggca aactggaaga
    28321 agatactctg cgcgccgttg gcgttttcgt tcagctcgaa acgcctatag ctttcgatgc
    28381 catcgacaac caaccggtgg acttactttt tgccctgctg gtgtcggcag accaaactaa
    28441 aacgcacctg catactctgt cgctggtggc gaaacgtctg gcggacaaaa ccatctgccg
    28501 ccgtttacgc gcagcccaga gcgatgaaga gctgtatcaa atcattacgg ataccgaagg
    28561 tactccggat gaagcgtagt tattcggtaa tgtctctttt agacgttgtg aggagaaaca
    28621 gtacatggta ctgatgatcg tcagcggacg ttcaggttca ggtaaatctg tcgccctgcg
    28681 tgcgctggaa gatatgggtt tttactgcgt ggataacctt cccgtagtgt tgttacccga
    28741 tctggctcga accctggccg atcgcgagat ttctgccgcc gtcagcattg atgttcgtaa
    28801 tatgccggag tcaccagaaa tattcgaaca ggcgatgagt aacctgcctg acgctttctc
    28861 accgcaactc ctgttcctgg atgccgaccg taatacctta attcgtcgtt acagtgacac
    28921 gcgccgactg catccgcttt ccagcaaaaa cctgtcgctg gaaagtgcta tcgacaaaga
    28981 aagcgatttg ctggagcctc tgcgttcgcg agcggatctg attgtcgata cctcagaaat
    29041 gtccgttcac gagctggctg aaatgctgcg tacccgtctg ctgggtaaac gtgaacgcga
    29101 actgaccatg gtctttgagt ctttcggctt caaacacggt atccctatcg atgcagatta
    29161 cgtctttgac gtgcgcttct tgccgaaccc gcactgggat ccgaaactgc gtccaatgac
    29221 aggtcttgat aaacctgtcg ccgcgttcct cgaccgccac acagaagtac acaattttat
    29281 ctaccagacg cgaagctatc ttgagctatg gttacctatg ctggaaacca acaaccgtag
    29341 ctacttgacg gtcgccattg gttgtaccgg cgggaagcac cgttcggtgt atattgcaga
    29401 gcaactggca gactacttcc gctcgcgcgg taaaaacgtc cagtcacgcc atcgtacgct
    29461 ggaaaaacgt aaaccatgac cgtcaagcaa actgttgaaa tcacaaacaa gctgggcatg
    29521 catgcccggc ctgcaatgaa gctgtttgaa ttaatgcagg gttttgacgc tgaagtgctg
    29581 ttacgtaatg acgaaggcac cgaggccgaa gccaacagcg ttattgcgct gctgatgttg
    29641 gattctgcca aaggacggca gattgaagtt gaagcgaccg gcccacagga agaggaagca
    29701 ctggccgccg ttatcgccct ctttaactct ggttttgatg aagattaatc ttcatcactt
    29761 tgacatacaa ctcccttcaa actccccccg acaataagaa aatcacgtac tgaaatcgtt
    29821 ctcaatcaac gtcatttgta cattttgtgc gcttttcact ttcagaagaa ccttaagaaa
    29881 accttaagag gcattgttta ggttttgttt aagttaatcg accatactgg agatcgtcag
    29941 aaaatatttc caggagatgg catgattcgc ttatctgaac aaagtcccct gggcaccgga
    30001 agacatcgga aatgttatgc gcatccggaa gatgcccaac gctgtatcaa gattgtctac
    30061 catcgtggcg atggtggcga taaagagatc cgccgtgagt taaagtacta cgcgcatctt
    30121 ggtcgccggt taaaagactg gagtggaata ccgcgctatc acggtaccgt tgagacggat
    30181 tgcggaaccg gttatgtcta cgatgtgatt gcagattttg acggcaaact ctccattacg
    30241 ctgaccgaat ttgctgaaca atgccgttac gaagaagaca ttgcacaact gcgccagcta
    30301 ctgaaacagc tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc gctgaagccg
    30361 cagaatatcc tctgtcaccg catcagtgag tcagaggtta tcccggtcgt ctgcgacaac
    30421 attggtgaaa gcacattgat tccactggcg acctggtcga agtggtgttg tctgcgtaag
    30481 caagagcggt tatggaaacg atttatcgcc caaccggcgc tggctattgc cctgcagaaa
    30541 gatctgcaac ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc ttaatccagc
    30601 tggtgttgtt gcataaacgg ctcaccgcct aactgataca tctgccgtaa aatccacgcc
    30661 tgacggctac gcacgtagcc cgatggcgag gagactttga aacgaagtgg attaggtaat
    30721 acagctgcca gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt gtggaaataa
    30781 cgttgtgccg cagcttcgac gccaaacaca ccgtcgccaa attcggcgat attcaggtaa
    30841 accgtcagga tacgcttttt gctccagact gtttctatcc ccagcgttaa tccagcttcc
    30901 agcccttttc gtacccagct acgcccatcc cataaaaaga gatttttggc cgtctgctga
    30961 gaaatcgttg aagcaccgcg aatacggttt tcattgcgct cgttgtgcgc cagggctttc
    31021 tcaatggaag cgacatcaaa gccccagtgc tcagaaaatt tctgatcttc tgcggcaatc
    31081 accgccagtc ccatccacgg cgagatttga tccatactga cccagtcaga atgtgccacg
    31141 taacgaaaat tgccatgcag ccaggcgctg acctgtcgct cgaccattac cgctgagaag
    31201 ggaacaggcg caacactaaa caacgcgatg cccccgcccc agaaaacggc gaggacaacc
    31261 attaacagca aaagaaaacg gcgaacgaaa ctaaacaccg ttaagcggct tttactcatt
    31321 cagccagaac cagcacgcgg gaaaccagct tatcaatgcc gctcgccgct tctgcaatgt
    31381 tctgcgccag catatatgct ggggtggtga caatcttatt gtcttcatca accacgatat
    31441 catcgacagg acagggcaca tgctccgcgc ccatctcttc cagcacttct gcggtatcga
    31501 tatcagtacc gatggtcaaa cgcagcggga aatcgaaaat tttcggcagc atcgccgggg
    31561 caatacacat aaaaccaagc ggttttccgg cctgatgcat cgcttgtgcc agcgccttta
    31621 attcacggtc aacggtgcat tcgctaccaa gagtggcaaa attgcttaaa ttcttcgccg
    31681 cgccaaaccc ccccggcaca atcaacgcat ccagttcagc ggcatcggcc tgggccagag
    31741 gacggatttc accgcgcgtt attcgtgccg cttcaatcag cacgttgcgc gtttccgtca
    31801 tcgcttcgcc agttaaatgg ttgataacat caacctgctg cttatccggt gcaaagcaga
    31861 ccgcctgcgc accgctgcgt gaaatagcta acaacgtcaa caccgcttca tgaatttcag
    31921 aaccgtcata gacgccgcat ccgctcagaa ttacgccaat tttcttcatt gtgatcatcc
    31981 tttcgcaatt gactgaaaca cattaatttt tttaataaaa atggtacgca tcacacattt
    32041 aactgattca tgtaacaaat catttaagtt ttgctatctt aactgcgtgc ggcctgaaaa
    32101 acagtgctgt gcccttgtaa ctcatcataa taatttacgg cgcagccaag atttccctgg
    32161 tgttggcgca gtattcgcgc accccggtct agccggggtc attttttagt ggcttttgcc
    32221 acccacgctt tcagcacttc tacgtcgtga cgccactctt ctttcatctc ttcaatccat
    32281 tcaccgacgt tatcttccca ggcaggaagg tcaggagact gaatttgctg acccagctgt
    32341 tgcagatggc gtaaccccac tgaccccgcc gcacctttaa ttttatgtcc ttcctcaaca
    32401 atgccttttt tatcctgcgc cgtcagattc gactccagca cgctgacata gcccggcatc
    32461 atcttctcaa acaccgctaa cccgtcggtg atcagcttcg gtcctacaag ttcgagatac
    32521 tgttccagca tgggaatatc gagcaatgct tctgatttac tgttctcttc tgtcgtcacc
    32581 gtactctcct catcatcctg ggtatcccag aatttcttga tcatcgcggt tagcgccgga
    32641 acagaaagcg gcttactcag cacatcatcc attccagcat tgaggtactc ttgtttgtct
    32701 ttcagcacgt tagcggttaa ggccaccagc ggcggtaaat cctctcgcgg ataacgcttc
    32761 gtcagttcac gagagatatc cagcccggtc atatctggca actgaatatc cagtaacacc
    32821 aggtcgtatt cgcccggttt aaacatctct agcgccgcct tgccggtcat ggcgacatca
    32881 acgctgttac ctaatttttc cagcacagaa cgcgcaacaa tcacgttcag ttcaatgtct
    32941 tccaccagca gcacattcag cgccggtaaa ggcatatcgt cttcatcaaa cgcatcatcg
    33001 acctcttctg ctaccgacgg tgcgtggatc gtcaacgtaa aggttgaacc tttgccctgt
    33061 tcgctggtaa ccgtaatatc gccgcccata tttttcgcca gacgacgaga aacggccaga
    33121 ccaataccgg tgccggtggc aggtttaccg ccatgactgt ctttcacctg gtaatacatg
    33181 gcgaaaattt tatccagctc atcctgcgga atgccgatac cagagtcttc cacttcaaaa
    33241 tgcagcatat cgccttcatc gtagcgcacg cgcacggtaa cctggccttg ctgggtgaat
    33301 ttgacggcgt tactgatgag gttccacagg atctgccgta aacgcgtccc gtcggtaatg
    33361 acctgatgcg gtaatggcag cgtcggctcc aggttaaagc gcaatccttt ttgttgcgcc
    33421 tgcaatgcgg agagattttc cagatcggca aggaagctgg tgaaatcaac cggttgatta
    33481 tcaagctgga ccttgcgccg ttccatctta tccatgtcga taatatcgtt aaagatattc
    33541 cccagcgtga cggccgaaac atggatggtc ttgagatatt tttcctgctc ggcggtgagt
    33601 tcggtatcca gcagaatgcg gctcagaccg acgataccgt tcagcggtgt acgcaattcg
    33661 tgactgatgg tggagataaa cgtcgttttg tcgcggctgg cccgttcaag cgcatcctga
    33721 taccgcttac gctcggtaat gtctcgacca aagcccatca aaccgtgacg tttacccacg
    33781 cggtcgtagt acggcacttt acggatttca aagcaggctt tgcgcccgtc cgggtaatcc
    33841 agccactgtt cataggtcag tgacacatta tgacggaaca ctttttcatc ggtttcaatg
    33901 acttttgcgg cggcttccgg tgagtaaaca tcaacaggtt tcaggtgaac cagttgtttt
    33961 tcgctttttc cggtcagcag ctccatcgcg cggttacagc cggaaaactc tttatcttcg
    34021 ttacgataaa aaaccaggtc gggtgaagca tcaaggaagg aacgtaagaa tgaggattgc
    34081 tgctcgagct gaatttgtgt ctcttcgcgc tctttgattt caattttcag ttggccgaag
    34141 gtttcctgca gttctgcttc cgctttttca cgaacggcga tttcctgatt tagctgggca
    34201 atattatctt ttaactgaac gttgaggctc aaatcgcgct cgcgcatctc ctccagtttt
    34261 tgcaccagcc gtgacagacg ttgtcgtgac tcctccagtt gctcgacgac caccgataga
    34321 aagtagaccg cccacggcgt aatcaacaaa ccaaagaaga tagaacgaat aacatcaatg
    34381 ctttcgacct gaccatgcag caccatggtt accgccattt gtaccacaat ggcaagaacg
    34441 acgagggcca gcgccagcaa cattgagaag cgcaccagac ctaacttcat catcaggtca
    34501 acataatact gcgccagcag acgaatttgc ttcattaggg aattccttca cgacaacctg
    34561 ccccaataat acccaattct gacagttacg ttataaattg tgtgagaaat gcggaattcc
    34621 cctcaacgac ctactccgtt ggaggtagcc acggacgtcc cagcgccgat ccctgtacgc
    34681 catgttcatt cagatagcga tccagctcga ccattcccgt ccatcgattt tcgcaccaca
    34741 gaggagcaag cagcgtcggg cgacgggcgc tggcggaaat gcggtggtag atcacttccg
    34801 gtggcgtatg gcgaatcatt tctccggcag tgagcgtgta atcctccagt tcaataccgt
    34861 tcaaacgccc cgcttcccag gctttcgcca taatgctgcc tttcacaata tgcaacggat
    34921 gtagctttat accgtcaaca ccggtttcca ctacccgctc cagcgtttgc agacattcag
    34981 ccagcccttc gcctggcaga ccgacaatta aatgcgagca caccttcagc ccgcgctggc
    35041 gtgccagctg cgtcgtgcgc tgataacagg cgaaatcatg accgcgatta atgcgatgta
    35101 gcgttttgtc gtgcacggtt tgcagcccca gctccagcca cacttcgtag ccctggtctt
    35161 tatattcgca aagcagatcc agcaccgcat ccggcacgca gtccgggcgc gtgccgacac
    35221 acaaaccgac aatattggcc tggctcaccg cctgttgata catcgaacgc agcacctgaa
    35281 cttcggcaaa ggtgctggta tacgcctgga aataggcgag gtaacgtttc gcgcgattaa
    35341 ttaaattcgc ctgatgcgcc agctgttcgg caatggaacg atgctgctgc gcttcgtcgg
    35401 caaacgaggc aacattacag aaggtgcagc cgccgcgccc gatggtgccg tcgcgattcg
    35461 ggcaactgaa accgccgtgc agcgtcagct tatgcacctt ttgcccataa cgacgggtga
    35521 gatcaccacc aaacatattg actaatttct gtaactgcat aatctgatag accgcgcctt
    35581 gaaaagaggc caaagcctgc catttttagc tcaattcggc gatgacctgg atcaatcgtc
    35641 ccggcccgct tttatcaact gcataatcaa tcaaaattac cgaaatttca tgcataatca
    35701 cataaatcac ttttgcttat cttgtgtcag atttttttat ctccttatgg attttaggca
    35761 aaaacagtag catgaaacgt cattaccaat taatgcagta taaaatgctg gttttctcgt
    35821 caattcaagg caggataagg gttaacacgc ctttatgaca gtcaggaatt gactgtttct
    35881 ctaacgactt cccttttagc cttaaagata aaatctattt taatttcagc catttaataa
    35941 agaattttgc gctaaagcac atttctgtac caataagctt gccatttgac ctgtatcagc
    36001 tttcccgata agttggaaat ccgctggaag ctttctggat gagcagcctg ctcatcatat
    36061 ttatgcagta attgagatcc cctcttcacc gtattaaccg atgcgaaaag gacaacaagg
    36121 gggcgaatgc gaggcgcgcg tatgacacgc aacccccgtc gccacgctct ttctgtaccc
    36181 gtgcgcagcg gttcggtagt ggggttcccg cagagcctgg gggaggttca cgatatgttg
    36241 tacgataaat cccttgagag ggataactgt ggtttcggcc tgatcgccca catagaaggc
    36301 gaacctagcc acaaggtagt gcgtactgca atacacgcac tggcccgcat gcagcaccgt
    36361 ggcgcgattc tcgccgatgg taaaaccggt gacggttgcg gcttgctgtt acaaaaaccg
    36421 gatcgctttt ttcgcatcgt tgcgcaggag cgcggctggc gtttagcgaa aaactacgcc
    36481 gtcgggatgc tattcctgaa taaagatcct gaactcgccg ctgccgcacg ccgcatcgtt
    36541 gaagaagaac tgcaacgcga aaccttgtcg attgtgggct ggcgtgatgt ccccactaac
    36601 gaaggcgtac tgggtgaaat cgccctctcc tctctgcccc gcattgagca aatttttgtg
    36661 aacgccccgg caggctggcg tccgcgcgat atggagcgcc gtctgtttat cgcccgccgc
    36721 cgcattgaaa agcgtctcga agccgacaaa gacttctacg tctgtagcct atcgaatctg
    36781 gtgaacatct ataaaggtct gtgtatgccg gcggatctgc cgcgctttta tctggatctt
    36841 gcggacctgc gtctggaatc ggccatttgc ctgttccacc agcgcttctc cactaacacc
    36901 gtaccgcgct ggccgctggc gcaaccgttc cgctatctgg cgcataacgg tgaaatcaac
    36961 accatcactg gtaaccgcca atgggcgcgt gcgcgtacct ataaattcca gacgccgctt
    37021 atccctgatc tgcacgacgc cgcaccgttc gtcaacgaaa ccggctctga ctccagttcg
    37081 atggataaca tgctggaact cctgctggca ggcgggatgg atatcatccg cgccatgcgt
    37141 ctgttagtgc cacccgcctg gcagaacaac ccggatatgg acccggaact gcgtgccttc
    37201 tttgacttta actccatgca tatggagccg tgggatggcc cggcgggcat cgtgatgtcc
    37261 gacggtcgtt ttgccgcctg taacctcgac cgtaacggtc tgcgtccggc gcgctacgtc
    37321 atcaccaaag ataagctcat cacctgcgcc tctgaagtcg gtatctggga ttaccagcct
    37381 gacgaagtgg tcgaaaaagg ccgcgtcggg ccaggcgaac tgatggttat cgacacccgc
    37441 agtgggcata ttctgcactc ggcagaaacc gatgacgatc tgaaaagccg ccatccatat
    37501 aaagagtgga tggagaaaaa cgtccgccga ctggtaccgt ttgaagatct gcccgatgaa
    37561 gaagtgggta gccgcgaact ggacgacgac acgcttgcca gctaccagaa acagtttaac
    37621 tacagcgcgg aagagctgga ctccgtaatt cgcgtactgg gcgaaaacgg tcaggaagcg
    37681 gtcggttcga tgggcgatga taccccattc gccgtgctct ccagtcagcc gcgcattatt
    37741 tacgactact tccgccagca gtttgcccag gtgactaacc cgccaatcga cccgctgcgt
    37801 gaagcgcatg ttatgtcgct cgccaccagt atcggtcgtg aaatgaacgt cttttgcgaa
    37861 gcagagggcc aggcgcaccg tttaagcttt aaatcgccga ttctgctcta ctccgatttc
    37921 aaacagctca cgacgatgaa agaggagcac taccgcgcag atacgctgga tatcaccttt
    37981 gacgtcacta aaaccacgct cgaagcgaca gtcaaagagc tgtgcgacaa agccgaaaaa
    38041 atggtacgta gcggcaccgt gctgctggtg ctctccgacc ggaatatcgc taaagatcgc
    38101 ctgccggttc cagccccgat ggcggttggc gcgatccaga cccgtctggt cgatcaaagc
    38161 ctgcgttgcg atgccaacat catcgtcgaa accgccagcg cccgcgatcc gcaccacttc
    38221 gccgtgttgc tgggcttcgg cgcgacggct atttatccat accttgccta tgaaacgctg
    38281 ggccgcctgg tagacaccca tgcgattgcc aaagattatc gtaccgtgat gctcaactac
    38341 cgtaacggca tcaacaaagg cttgtacaaa atcatgtcca aaatgggcat ctccaccatc
    38401 gcctcttacc gctgctcgaa actgtttgaa gcggtcggtc tacacgatga tgtagtgggc
    38461 ctgtgcttcc agggggcggt cagccgcatt ggtggggcaa gctttgaaga cttccagcag
    38521 gatctgctga atctgtcgaa acgtgcctgg ctggcgcgta agcccatcag ccagggcggc
    38581 ctgctgaaat acgtccacgg cggcgaatac cacgcctaca acccggacgt ggtgcgcacg
    38641 ctgcaacaag cggtacaaag cggcgagtac agcgactatc aggaatacgc gaagctggtt
    38701 aatgagcgtc cggcaaccac gctgcgcgat ctgctggcaa ttacgccggg tgaaaacgcg
    38761 gtcaacattg ctgatgttga accggcaagc gaactgttta aacgcttcga taccgctgcg
    38821 atgtctatcg gcgcgttaag cccggaagcc cacgaggcgc tggcggaagc gatgaacagc
    38881 atcggcggta actcaaactc cggtgaaggc ggtgaagatc cggcgcgcta cggcaccaac
    38941 aaagtgtcgc gcatcaagca ggtggcttcc ggtcgctttg gggttactcc ggcgtatctg
    39001 gtcaatgccg acgtcattca gattaaagtc gcccagggcg cgaagccggg cgaaggcggt
    39061 cagttgccgg gtgataaagt cactccttac atcgccaaac tgcgctattc ggtgcccgga
    39121 gtgacgctga tctccccgcc gccgcaccac gatatctact ctatcgagga cttagcgcag
    39181 ctcattttcg acctcaagca ggttaacccg aaagcgatga tctccgtgaa gctggtttcc
    39241 gaaccgggcg taggcaccat cgcgactggc gtggcaaaag cttatgccga tttaatcacc
    39301 atcgcaggct atgacggtgg caccggcgca agcccgctgt cgtcagtgaa atacgcaggt
    39361 tgcccgtggg agctggggct tgttgaaacc cagcaggcgc tggttgccaa tggtctgcgt
    39421 cataagatcc gtttgcaggt cgatggcggc ctgaaaaccg gtgttgatat catcaaagcg
    39481 gcgattctcg gcgcagaaag cttcggcttc ggcactggcc caatggtcgc gctcggctgt
    39541 aaatatctgc gtatttgcca cctgaacaac tgcgcaacgg gtgtggcaac tcaggatgac
    39601 aaactgcgta agaatcacta tcacggcctg ccgttcaagg tgacgaatta ctttgagttt
    39661 atcgcccgtg aaacccgcga gctgatggca cagctggggg taacgcggct ggtggatctg
    39721 attggtcgca ccgacctgct gaaagagctg gacggtttca ccgccaaaca gcagaaactg
    39781 gcgctgtcga agctgctgga gaccgccgaa ccgcatccag gtaaggcact ctactgcacc
    39841 gaaaacaacc cgccgtttga taacggcctg ctgaacgcgc agttgctgca acaggcgaaa
    39901 ccgtttgtcg atgaacgcca gagcaaaacc ttctggttcg atatccgcaa taccgatcgc
    39961 tccgtgggcg cgtcgctttc aggctatatc gcccagacgc acggcgatca ggggctggca
    40021 gccgatccta tcaaagcgta cttcaacggc accgcaggcc agagtttcgg tgtatggaac
    40081 gcgggcggcg tggaactgta cctgaccggg gatgccaacg actatgtcgg taaaggcatg
    40141 gcgggcggct taatcgccat tcgtcctccg gttggttccg ccttccgcag ccatgaagca
    40201 agtattatcg gcaacacctg cctgtatggc gcgaccggtg gtcgtctgta tgccgcaggc
    40261 cgcgcgggtg aacgtttcgg cgtgcggaac tccggtgcta tcaccgtggt agaaggcatt
    40321 ggcgacaacg gctgtgaata tatgacgggc ggtattgtct gtattctggg taaaaccggc
    40381 gttaacttcg gtgcgggcat gaccggcggc ttcgcttacg ttctcgatga aagcggtgat
    40441 ttccgcaaac gcgttaaccc ggaactggtc gaggtcttaa gcgttgacga tctggcgatc
    40501 catgaagagc atctgcgcgg tcttatcacc gagcatgttc agcatactgg ctctcagcgc
    40561 ggtgaagaga ttctggcgaa ctggtcaacc ttcgccacta aatttgcgct ggttaaaccg
    40621 aagtccagtg atgtaaaagc actgctgggt catcgtagtc gtagcgcagc agagttgcgc
    40681 gtgcaggcgc agtaaggggt agcaacaatg agtcagaatg tttatcaatt tatcgacctg
    40741 cagcgcgttg atccgccaaa gaaaccgctg aagatccgca aaattgagtt tgttgaaatt
    40801 tacgagccgt tttccgaagg ccaggccaaa gcgcaggctg accgctgcct gtcgtgcggc
    40861 aacccatact gcgagtggaa atgtccggta cacaactaca tcccgaactg gctgaagctc
    40921 gccaacgagg ggcgtatttt tgaagcggcg gaactgtcgc accagaccaa caccctgccg
    40981 gaagtttgcg gacgagtctg cccgcaagac cgtctgtgcg aaggttcctg cactctgaac
    41041 gatgagtttg gcgcggtgac catcggcaac attgagcgtt atatcaatga taaagcgttc
    41101 gaaatgggct ggcgtccgga tatgtccggc gtgaagcaaa ccggtaaaaa agtggcgatt
    41161 atcggcgcag gcccggcagg tctggcgtgt gcggatgtcc tgacgcgtaa cggcgtaaaa
    41221 gccgttgtct tcgaccgtca tccagaaatt ggcgggctgc tgaccttcgg tattccggcc
    41281 ttcaagctgg aaaaagaggt aatgacgcgt cgccgtgaaa tcttcaccgg catgggtatt
    41341 gaattcaaac tcaataccga agtgggccgc gacgtgcagc tggacgatct gctgagtgat
    41401 tacgatgccg tcttccttgg cgtcgggact tatcagtcaa tgcgcggcgg gctggaaaac
    41461 gaagacgccg atggcgtgta cgcagcgctg ccgttcctta tcgccaacac caaacagtta
    41521 atgggctttg gtgaaacccg cgacgaaccg ttcgtcagca tggaaggcaa acgcgtggtg
    41581 gtccttggcg gtggcgacac tgcgatggac tgcgtgcgta cgtccgtgcg ccagggagcg
    41641 aagcacgtta cctgtgccta tcgtcgtgat gaagagaaca tgccgggttc ccgccgcgaa
    41701 gtgaaaaacg cgcgggaaga aggcgtagag ttcaaattca acgtccagcc actggggatt
    41761 gaagtgaacg gtaacggcaa agtcagcggc gtaaaaatgg tacgtaccga aatgggcgaa
    41821 ccggatgcca aaggccgtcg ccgcgcggag atcgttgcag gttccgaaca tatcgttccg
    41881 gcagatgcag tgatcatggc gtttggtttc cgtccacaca acatggaatg gctggcaaaa
    41941 cacagcgtcg agctggattc acaaggccgc atcatcgccc cggaaggcag cgacaacgcc
    42001 ttccagacca gcaacccgaa aatctttgct ggcggcgata tcgtccgtgg ttccgatctg
    42061 gtggtaaccg ctattgccga aggtcgtaag gcggcagacg gtattatgaa ctggctggaa
    42121 gtttaagctc tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa attctccttt
    42181 ctttctggaa agcgcctcgc ataatctgaa gaaaaccaat gtaatacgct catctggatt
    42241 gttaccggta gatgcccggc gtatagctga ttccattatt caacgcagcg ccatataaag
    42301 ggacagataa tggaatcgct ctcagagaga acctcaacag gctaccagca aatccacgac
    42361 ggtattattc acctggtcga tagcgcccgg acggaaacgg tacgtagcgt taacgcgctg
    42421 atgaccgcga catactggga aattggccga cgaattgtcg aatttgaaca aggtggcgag
    42481 gcaagggctg cgtatggtgc gcagctaatc aagcgactat caaaggattt aagtctaagg
    42541 tataagcgtg gtttctctgc aaaaaactta cgccaaatga ggctttttta cctctttttt
    42601 caacatgttg aaattcgcca gacagtgtct ggcgaattaa caccattggg aattccgcag
    42661 acaccatctg cagaattccc atcggcaaag atttggcaga cgctgtctgc caaattgaga
    42721 ggctgtttga tatttccaca gcttttgtat tgcatctcct attttcggtt tgtatattca
    42781 tgcaaataaa cgaaggaaag ttgccgtgat ttacattgaa cagcttgaat taatccataa
    42841 aagtggtgat gtgttgtatc cagtaaaaat aaccagaaaa tcatcaggaa aaactgcctt
    42901 ccaccttgtt ccattcggct taaataagac tcacgacctt ctagaggtgg aagatgcttc
    42961 agaagcaatt cggctggtga ttgatgaacg tcattccatt cgctgcagta cacttacagc
    43021 aactattacg aataaaaagg gcaagcgaat taagcggaca ggtatataca gtatcaaggg
    43081 tgttaacata aaagaataca acgtcaggta atggcacaga gcggtccgtc agttcaggtt
    43141 tggctctgtg ccaaagatgg gtcaggtcac acctgatcta ataaatttaa gccattaaat
    43201 cagccttaac cttcaccacc acctttttaa tctcacccgg ctcgccaaca acgcatcccg
    43261 gcttatgcgg ctctcctagc ataaacacgg cgaacattcc cggtttcagg atgatggctt
    43321 gctcgttttc aatggtgctg caaagctggt aatcatcctc atggtggaac tcttcacact
    43381 gacgcgcagt gcctgccatg ccaaacagaa tccgttcttc accgtttaat aacagctgga
    43441 tatcaatgta ttgctcgtgc agctccgctt ttttctcaac gggcgattga gtgttaaacg
    43501 tcatgacatt cataaaaata ttgtcgccct gtaattcgta acgacccggc gctttttctt
    43561 gcggtctggc agctaatgcc agcgttaacg cgtcctgtaa cgcaggatgt aacccagcag
    43621 acggtaatga ccgtacttca cccatcatca taatttttct ccctgggcca acagcgctgc
    43681 cccaagtaaa cctgcatcat ggcggtagtg cgccgccagt aaatcaacat gaaataccgc
    43741 tggctcctgc gccagatacg tttccaccaa cgccagatac ccttctgcca gaccaacgct
    43801 gccaccgacc accacgcact ggcaatcagt tgtggcttta atatcagcga tcagccttgc
    43861 cagcacatgt gcggagcggt gaatcagttg ctgcgcctgc tcgtcgccct gcccggcgcg
    43921 tgtgaaaata gttctcgcat ccgcgcccat gagttcacct tgcgctgccg ctgcaatacc
    43981 gcgaccagaa gcaatcgctt ccacgcaacc tgtgcgtcca cagccgcaga ctgggccgtg
    44041 tggatcggca agcgtatgcc cgatatgccc cgccagaccg ccagggccgg taagcagttt
    44101 accgccgctc actacaccgc cgccgacgcc agtggaaacg gtgataaaca ccatctcggt
    44161 tacatcgcct tccagcgcct gatactctgc ccatgctgcg gcctgcgcgt cgttaatggc
    44221 aatggtcggc aaattggtaa gttgttccag cgttttgact aacggaaagt gtagcaatcc
    44281 accgagatta tgcggattca gcgccagcaa actgccgtcg cgaataattc cggttgaagc
    44341 gatggcaacc cgctgcgcat gagcttgcaa cggagagact aatgcggata aggcatcacg
    44401 caaggcttct ggtgtctggc tggctggcgt aggaagttca cgacgatcgc ggatctgccc
    44461 gtcagcgcca atcagcgcgg cggcaagttt agtaccgccg atatcaatcg ccagtgtggt
    44521 catagcaccg cctttttcat cgctgtgttg taccactgac aaatgtgttc aagacgcgta
    44581 atggcagaac cgaccgtcac cgcccacgcg ccgtggcgca tcgcatctgc cgcctgagca
    44641 ggcgtgttgt aacgcccttc ggcaatcacc cgacatccgg cttcgctcaa cgttttcacc
    44701 agcgccagat ccggctcttc tggcgtttca ggcgtggtat agccagaaag cgtagtgcca
    44761 ataatttcgg ctcccagctt ttggcatgcc aggccgtctt ccggcgttga gcagtcggtc
    44821 atcgccagta aaccgtgatg atgtatacgt gccagcagcg tttcaacagg caccggacgc
    44881 gggcggtcgg tgccgtcaat ggcgataatg tccgcgcccg cctgcgccag cgcatcgaca
    44941 tcttcaatat aggccgtgat gcgtaccgga gaatcctcca gatcgcgttt cacaattcca
    45001 ataatcggca cgctcaccac cgcacgcgtg gcttgcagat ttgccacacc ttcgatgcga
    45061 atggcaaccg cgcccgcctg ctctgccgct aatgccatgg ctgcgacgat ttcgggttta
    45121 tcgagcgggc tgtcaggaat cggctggcag gagacaatca ggccaccgtt agcagcgatt
    45181 ttttgatcca gttgtgcaag taacgacata catcttccct tagcgaaagg cccggtacat
    45241 agaccgggca acaggattaa cttttggttt tgactaaatc gtttttggcg ctgccaaatg
    45301 gcacggcacc gctaaatggt ttgccgtcga tagcgtcatg agtacgcaat gcttccgggc
    45361 gcagccaacg ctgaacgcgg gaaggcatat ccagaccaat cagcaggatc accacaaatg
    45421 tcaggctgaa cgagagcgat gccagcgcag tacccagatc cagacgttga gcgatcaacg
    45481 cgccgatgat tggggccagt gcgccaccca atgcgccaac gttgtaggta aagcccaggc
    45541 ccgctgcacg ctggtcggta tcgaaataac cgccaatcag tttcggtaag atcccggcga
    45601 tcccttgtcc aagcatttgc tggaagaaca gtaacagacc gagcacccag acgtttgcgc
    45661 cgccaatcgc aaataccgga ataatcagca gctgcgaggc cagcaggcta caaacgtacg
    45721 ctttgcgggt tcccagccag tcaccgagga agcctcctac gcagcatccc acggccgcgc
    45781 caaagccact aaagaacagc acattggcga cagtatgcgg gttataagcc agatcggttt
    45841 tcagatacgt tggcagcagc gcctgaatcg gccatgagta gaggaaagca aacaacacga
    45901 ccaccatcag cattacgccc gttggccagc gtttgcctgt actctgcacc ataaagctga
    45961 taaagattgc ggcgcataac agcccaagaa cagcgacgat cgcggcattt tgcaggttac
    46021 cggcgaagca gaaccacagc gcagtagccg ccgccagtgt cattacgata ttggcaatgc
    46081 gatgttcacc acggtagaga atatccacca ttgtgcgtac tggtgcttta cctgcgtgtt
    46141 tctctttcca gtcttccgct tccgggatgt ttttacgcag ccagagagca aagatgattg
    46201 gcaaaatgcc gataaagaac agcgcacgcc aaccccagac cggaaccacc aggctataga
    46261 cctgagcggc aacgacggcc cccacagaga agcctgaaat caaaaaacca ctggctttgt
    46321 tacgcagatg ttttggccag ctttcaatga cataggtggc gctggaaccg tattcacccg
    46381 ccatccccat gccgatgacc agacgagcga taaacatggt gatgtagcct ggcgcaaagc
    46441 cgcaggccag cgtcccggcc gagaagagaa cgatgctggt gaccattgcc agacgacgcc
    46501 cgtagcggtc acccatagcg ccgagcatca ggccgccgaa ccagcgagag ataaaggctg
    46561 cggagatcag acttgccgcc tgcaccgtcg tcagcccgaa ttcaccctgt acttcggtga
    46621 gtaccagggc gattaaaacg aaatcaaaac cgtcaagcag atatcccaac caggcagcgg
    46681 aaaatgcgcg ccattgtgca cggttgagat ggcgatacca cgggatattc tgggttgtag
    46741 tactcattgt gagtctcccg cggtgggcga tgcccacacg ctttggtatg aaaattgtag
    46801 ggtacagatg cgtttatttc ccctcacccg gtaggggcga gcgaggggaa aacaactcac
    46861 ccgcgctctt gcatcaactg ctgggccagc gccttcagtt ctggcagata tttttcatct
    46921 accggtccaa acggtttgcg gcacagcggc acagaaacga catccatata atgcaggaca
    46981 gttttcaggc cgcggaatac gccagttttg atcagtaaat caatgacttt gttgcactca
    47041 gtttgcagtt tctgcgcggt ctggatatcg ccttctttca gcgccttcac gatcccctga
    47101 tatcgccagc ccatgatgtt gtaagtactg ccgataccac catcagcgcc tgccagcaag
    47161 ccggaagcga agatttcgtc gtaaccgtta tagagcacca gatcaggatg ttcacgacgg
    47221 atctgttcca tctgatagag atcgccagaa gtctgtttca gcgcaccgac gccaggcaac
    47281 gtaaccaggg tgttgatctg atccagggtc agttttaccc cactcagggc tggaatgttg
    47341 tacacaacca tcggcaaacc atccgctgaa tcaataattg cccgatagtg atcgcagtgt
    47401 tcttcaaagc tgaaaggata gtagaacggc gtgacggcgg agacggcatc gaagccataa
    47461 cgtttagccg atgccgcaag ttgttggctt tcggcggtgc tgacgcaacc gacgtgggcg
    47521 atgagtttaa tcttaccttt cgcctcttcg gcgacgattt ccagtacctg ttcacgctcg
    47581 gaaaggcttt gtacaaaggc ctcgccggtc gaaccaccca cgtataaacc gtcgatgccc
    47641 tgctgaatat tgaactgaac caggcgacgc agactcgctt tatccagtgc ttgttgttgg
    47701 tcaaaaggag tcaggagtgc agccattacg ccacgtaaat tcgttgccat aaatacctct
    47761 gaagtgatac ttgtctgata aacgatatac ctttatacct gttataccag atcaattaag
    47821 caacacccca tacagaaagc ttataatgcg atctgcttca ctaaagtggc attatttctt
    47881 tttgttggtg gtctgaccga aagcgtgcca ggtagcagag acgctgttga gatgcgattg
    47941 caacgcacga tcggcttcgt caggatcatg acggcggatc gcatcaacga tcgcaatatg
    48001 ctgttgatag ctaacgttgt tatgctcgtg cagagcctga tcagccacgg ttggacgtgc
    48061 ggcgatcagc cagtcgagca gggcaacgtg gatcgccatg aagattgggt taccagggat
    48121 ctccgccagc acgcggtgga aatcaacgtc tgaacgaatg aatgccgcgt tgttatccag
    48181 cgactgactg ttgatttcca gtgcttttgc cagcaaatcg atttgctcat cggtggcatg
    48241 ttcagccgca tagcgcacca gactggattc aaagaacaga cgtaattgtt cgaaatgggc
    48301 aatcccaccg ggatgagaaa ggaaatcttt cgccatgccg gaaaactcac cgatgatagt
    48361 gtccgcagaa ggacgcgaga cgcgagcgcg ttcgccgttg tttatttgca ccagaccttt
    48421 gcgttttaac gctgccagcg cttcgcgcac agaaggacgc ccgacgttaa agaacgccat
    48481 cagttcgcgt tcagacggta attgttcacc ttcgccaaat tcacgacggc ggatcatctg
    48541 ttccagctct tcttccacca tttcggagag ttttttacgc gccagcgggc ggctacgcaa
    48601 gttgcgacca attgcaggtg aagaatcttc ggtttgcgaa tcaaatgcgt tcataaggcc
    48661 cattctgtaa ggtcagtgtg attaacatca tcagtgacat cctatcacag gattgaaagt
    48721 aggggaaaat ggcaggtttt tctctttgtg cctcatcatt accataatta acggaataat
    48781 taactattgc gaaaaattaa tgtaacgcag ataaacacat cccgattgaa ttattcataa
    48841 gactattcac aagcattatg aatattatga atgtgttctt acaaaataat cataagcgca
    48901 tattttttaa tgaaaaatca catcacctac aattaaaaac acgacatccg caccataaat
    48961 agacttgcaa aaaatataac atcattgttt tcaatctgcc gtttatggga ttgaccgttt
    49021 tcttttgaca cggagttcaa caatgttcgg cataattata tctgtcatcg tattaattac
    49081 gatgggctat ttgatcctga aaaactacaa acctcaggtg gtgctggctg ccgcaggtgt
    49141 cttcctgatg atgtgcggag tctggttagg gttcggtggt gtactcgatc ccgccaaaag
    49201 cagcggctac ttgatcgtcg acatttataa tgaaatcctg cgcatgctgt ccaaccgcat
    49261 tgccggattg gggctgtcga ttatggcggt gggcggttat gcccgctaca tggagcgcat
    49321 aggagccagt cgcgcgatgg tgagcttgct aagccgcccg ttaaaactca ttcgctcgcc
    49381 gtatattatt ctgtcggcaa cttacgtcat cggccaaatc atggcgcagt ttattaccag
    49441 cgcctccggt ctgggtatgt tgctgatggt caccttattt ccgacgctgg tgagtctggg
    49501 agtaagtcgc ctctctgcgg tggcggttat cgcaaccacg atgtccattg agtgggggat
    49561 tctggaaacg aactccattt ttgcagccca ggtcgcggga atgaaaattg ccacatactt
    49621 cttccactac cagcttccgg tcgcctcttg cgtcattatc tcggtggcga tctcccactt
    49681 tttcgtgcaa cgcgcttttg acaaaaaaga taaaaatatc aatcacgaac aggcagagca
    49741 aaaaactctc gataatgtcc cgccgctcta ttacgccatt ttacctgtga tgccgttaat
    49801 cctgatgctc ggctcgctgt tcctcgccca cgtcgggctg atgaagtcag aactgcatct
    49861 ggtggtggtg atgttactaa gtttgacggt gacgatgttt gttgagttct tccgcaagca
    49921 taacttgcgc gaaacaatgg acgatgtgca ggcgtttttt gacggcatgg gtacgcagtt
    49981 tgccaacgtg gtaacgctgg tggtcgcggg tgaaatattt gcgaaaggct taacgacgat
    50041 tggcactgtt gatgcggtta tcaggggtgc ggagcattct ggtctgggcg gtattggcgt
    50101 gatgattatt atggcgctag tcattgccat ttgtgccatt gtgatgggct ctggcaatgc
    50161 gccgtttatg tcatttgcca gtcttattcc gaatatcgca gccggactac atgtaccagc
    50221 ggttgtaatg attatgccga tgcattttgc cacgacgcta gcgcgcgcgg tttcgccgat
    50281 tactgcggtg gtgaagcgga cagcgatccc catggcagtc ggtttcgtgg tgaatatgat
    50341 tgccacaatc acgctatttt attaagtcat taaaaagaca aaacaggccg cctgggcctg
    50401 ttttgtatta cttcacaaca cgtaatgccg gtcgaccacc gcgtggtggc tgcggaggtt
    50461 catcgtcagg atgagtgtca tcatcgtgat ctggcttgtc gccatcaata accgacataa
    50521 cggtttcgtt gtctgccgat gcctcttcat cattcatgat gctggtatct tcatcgtagg
    50581 cagcttcagg ctcaaacatc gtgcctgcgc cattttcacg ggcgtagata gccagcacgg
    50641 cagccagcgg cacagaaacc tgacgcggaa tgccaccaaa gcgcgcgtta aagcgcacct
    50701 catcattcgc cagttccaga ttgccgacag cacgcggcgc aatgttgagt acgatttgcc
    50761 cgtcacgcgc atattccata ggaacctgca cgccagggag cgtcacatcc accaccaggt
    50821 gcggcgtgag ctggttatcc agcaaccact catagaatgc acgcagcaga tagggacgac
    50881 gtggtgttag ctgtgacaaa tccatacaga ttaactccgg cccagacaca tttcacgttc
    50941 tgcttcagtt aaagaagcaa ggaaagagtc acgctcaaag acgcgggtca tatagccttt
    51001 cagctctttc gcacccgggc cgctgagctc gatacccagt tgcggcagac gccacagcag
    51061 cggagcaaga tagcaatcga ccaggctgaa ctcatcgctc aggaagtacg gcttctgacc
    51121 gaagaccggc gcaatcgcca gcagttcttc gcgcagttgc ttacgtgcgg catctgcttc
    51181 agaagctgaa ccgttgatga tggtgttcat cagcgtgtac cagtcttttt cgatgcgatg
    51241 catgtacaga cggctttcac cgcgagctac cgggtaaaca ggcatcagtg gcggatgcgg
    51301 gaaacgctca tccagatatt ccataatgat gcgagattcc cacagggtca gctcacgatc
    51361 caccagggtc ggaacgctct gattcgggtt gaggtcaatc agatcctgag gcggattgtc
    51421 cttttccacg tgttcgatct cgaaacttac acctttctca gccagcacaa tgcggacctg
    51481 atggctatag atgtcagtag gaccggaaaa cagcgtcatt accgaacgtt tgttggcagc
    51541 gacagccatg aaaacctcca ggtatagtca gaatttttac tgctaccagc caccaggtgg
    51601 ccagtcagaa gttgtgttac ccaataagga acgactctct ttgttcgaaa atcaaacaaa
    51661 aaatgagcaa tacccgacat ttgggcagaa aattggatga tagtttacca gattttgcga
    51721 ccattgtggt gagtggatgc cggaaatggg gaaaaagaga tgcgctttag tctgaaatag
    51781 ttgacttaga cccttattgg cgatgtggtt tttgttttgc ctgtctttca gacggcagca
    51841 aaaagcaact tcccattttt tacgctgatt cagaatttag ccataaaaaa acccgccgaa
    51901 gcgggttttt tcgtaaattg ttttctgccg gagcagaagc caattaacgt ttggagaact
    51961 gcggacgacg acgtgcttta cgcagaccga ctttcttacg ttcaacctga cgagcgtcac
    52021 gagtaacgaa gccagcttta cgcagttcag aacgcaggga ctcgtcgtat tccatcagag
    52081 cgcgggtgat accgtgacgg atcgcaccag cctgaccaga gataccacca cctttaacgg
    52141 tgatgtacag gtccagtttc tcaaccatgt cgaccagttc cagcggctga cgaactacca
    52201 tgcgggcagt ttcacgaccg aagtactgtt ccagagaacg ttggttgatt acgattttac
    52261 cgttgcccgg tttgatgaaa acgcgagctg cggaattttt gcggcgacca gtgccgtagt
    52321 attgattttc agccattgcc tataatcccg attagatgtc aagaacttgc ggttgctgtg
    52381 ccgcgtggtt gtgctcgtta cccgcgtaaa ctttcagttt acggaacata gcacgaccca
    52441 gcgggccttt tggcaacatg cctttaaccg cgatttcaat cacacgctca ggacggcgag
    52501 caatcatctc ttcaaagctc acttgtttga taccaccgat gtggccggtg tggtgatagt
    52561 acactttgtc agtacgcttg ttgccggtta cagcaacttt gtcagcgttc agaacgatga
    52621 tgtaatcacc ggtatctacg tgcggagtgt attccgcttt gtgcttaccg cgtaggcgac
    52681 gagccagttc agtagccaga cggcccagag ttttaccggt cgcgtcaaca acataccagt
    52741 cgcgttttac ggtttctggt ttagctgtaa aagttttcat taaaagctta cccaataaat
    52801 agttacacgt tggtgaacac ccaaacgtct tcaattgttg aggttcacac gacaaagtcc
    52861 ggcaaaccta ccccttcgaa tagcctatgc cagcacacaa aaagttttgg gaaaaaaact
    52921 ttcttgtaac gtggggtcgc aggattatag agaagtcggg gtcaaagatc gacccctttt
    52981 tgtgatttgt gacaggtttt aacccgccaa atgctcgcgc ttcagatact cttcgctttg
    53041 catctcttgc agacgtgaca ggcaacgctg gaactcaaac ttcagccgat cgccctgata
    53101 aatttcatac agcggcactt ctgcactcac cactaatttg acatggcgct cgtaaaactc
    53161 atccaccagc gcaataaagc gccgcgcttc gctctccatc aaccgcgtca taactggtac
    53221 atcaaacaac atgaccgtat gaaagagacg tgagagcgca atatagtcat gctgactgcg
    53281 ggcgtcgacg cacagcgtag taaaagagac cgccagcgtc tggttctcga cgcccattgt
    53341 cgctaatggc cgatggttga tttctaacgt cggtgaattt tctcgtttcc cccccgccag
    53401 cgccaaccat agtttatcca tttgcgcccg ggtttcatcg tggagtggcg aaagccacag
    53461 atgcgcctga gtgagtgtac gcagtcgata atcaacacca gcgtccacgt tcattacatc
    53521 acaatgctgt ttaatggcat cgattgcagg cagaaaacgc gcacgttgca agccatttcg
    53581 ataaagttcg tccggcggaa tatttgacgt cgctaccagg gtaataccgc gggcgaacaa
    53641 ggctttcatc agaccgccaa gtagcatggc atcggtaata tcagaaacaa aaaattcgtc
    53701 aaaacagagc acgtcagttt cggctttaaa gcgatcggca ataatttcca gcggatcggt
    53761 ctgcccctgt aaggcagtta gctcttcatg cacccgcagc ataaaacggt gaaagtgcag
    53821 gcgctgtttc cgctctcccg gcaggctttg atagaaaagg tccatcagcc aggttttccc
    53881 gcgtcctaca ccgccccaca tatataagcc acgcactggc gtatgctttg tgtcttcgcg
    53941 tttaccccac agcttaccga cccgcgccat tagcccactc gtcctggggg ctggtggcgt
    54001 gctattgatg agttcctgat aaataatttc caggcggctg acggcctctt tttgaacgtc
    54061 gtcgggttga tggctgcctt cattgagcgc cttcaggtat tgcgatgttg gggtaacgct
    54121 ttgcatgatc ttattgttat tccttgaata atcggtgcgc cgttgttcac ggttgacgaa
    54181 aaaaaggccg ttctacacta cgcgatatgc agtcgggatt ccacttctgt ggaattaacg
    54241 gttatagtgg cataatcagc cgcaggcatg gagcctgaag ccaacaccct acggaaacaa
    54301 aagacaacgg gagatgttca tgacctggga atatgcgcta attgggttag tcgtcggcat
    54361 cattattggt gctgtggcca tgcgttttgg taatcgtaaa ctacgccagc aacaggcgtt
    54421 gcagtacgaa ctggaaaaga ataaagctga actggacgag tatcgcgaag agctggttag
    54481 ccactttgcc cgcagcgcgg aattactgga taccatggcg cacgactatc gccagctgta
    54541 tcagcacatg gcgaaaagtt ccagcagcct gctgccggaa ctgtctgctg aagcaaaccc
    54601 gttccgtaat cgtctggcag agtctgaagc cagcaacgat caggcaccgg tgcagatgcc
    54661 tcgcgactat tctgaaggcg catccggcct gctgcgtact ggcgcgaagc gcgactaatt
    54721 tatttttcgg gcgcagccat tgcgccctcc tcttctctcc ctccccgact atcatttaat
    54781 ctggtgtctc attgttagcc gtctgaaaat tcaataacat caaactgttt tgaatctctt
    54841 ttcttatcat tcaggtacga gagcaggaat aatgaaaaaa caaccccagc tgttgagtgc
    54901 attagcgtta agtgtcgggt taactctctc ggcgtcattt caggccgtcg cgtcgattcc
    54961 aggccaggtt gccgatcagg cccctctccc cagtctggct ccaatgctgg aaaaagtgct
    55021 tccggcagtg gtgagcgtac gggtggaagg aacggccagt cagggacaga aaatcccgga
    55081 agaattcaaa aagttttttg gtgatgattt accggagcaa cctgcacaac ccttcgaagg
    55141 tttaggctcc ggtgtcatca tcaacgccag taaaggctat gtgctgacca acaaccatgt
    55201 gattaatcag gcacagaaaa tcagtattca gctcaatgat gggcgcgagt ttgatgcaaa
    55261 actgattggt agcgatgacc agagcgatat cgccctgtta caaattcaaa acccgagcaa
    55321 attaacgcaa atcgctattg ccgactccga taaattgcgc gtcggtgatt ttgccgtagc
    55381 ggtcggtaac ccatttggcc ttgggcaaac cgccacctct ggcattgttt ccgcattagg
    55441 acgcagcggg ttgaatcttg aaggtctgga aaactttatc cagacagatg cttccattaa
    55501 ccgcggtaac tccggcggtg cactgttaaa ccttaacggt gagttaattg gcatcaacac
    55561 tgcaatcctt gcgcctggcg gcgggagcgt cgggattgga tttgccatcc ccagtaatat
    55621 ggcgcgaaca ctggcgcagc agcttatcga ctttggtgaa atcaaacgcg gtttgttagg
    55681 catcaaaggc accgagatga gtgccgatat cgccaaagcc ttcaaccttg acgtgcagcg
    55741 tggcgcgttt gtcagcgaag tgttgccagg ttctggctcg gcgaaagcgg gcgtcaaagc
    55801 gggcgatatt attaccagcc tcaacggcaa accgctgaat agctttgctg agttgcgctc
    55861 tcgtatcgcg accaccgagc cgggcacaaa agtgaaactt ggcctgctgc gtaacggcaa
    55921 accactggaa gtagaagtga cgctcgatac cagcacctct tcgtcggcca gcgctgaaat
    55981 gatcgcgcca gcgctggaag gtgcaacgtt gagcgatggt cagctaaaag atggcggcaa
    56041 aggtattaag atcgatgaag ttgtcaaagg aagcccagct gctcaggctg gcttgcaaaa
    56101 agacgatgtg atcattggcg tcaaccgcga tcgggtgaac tcgattgctg aaatgcgtaa
    56161 agtgctggcg gcaaaatcgg ccatcatcgc cctgcaaatt gtacgcggca atgaaagcat
    56221 ctatctgctg atgcgttaat gtcgtaaacc gggcatcagg cttacgtgtg atgtccggtt
    56281 aactcgtggt atgctgctgc cgttcccttt tttaatgacg cctccatcat gtttgtgaag
    56341 ctcttacgtt ccgttgcgat tggattaatt gtcggcgcta ttctgctggt tgccatgcct
    56401 tcgctgcgca gccttaaccc gctttccact ccgcaatttg acagtaccga tgagacgcct
    56461 gccagctata atctggcggt tcgccgcgcc gcgccagcgg tggttaacgt ttacaaccgt
    56521 ggtttgaaca ccaactctca caaccagctt gagatccgca ccctgggatc cggtgtaatc
    56581 atggatcaac gcggttatat catcaccaat aaacacgtca tcaacgacgc cgatcagatc
    56641 atcgtcgcct tacaggatgg tcgtgtattt gaagcattgc tggtgggatc tgactctcta
    56701 accgatctgg cggtacttaa aattaatgcc actggcggtt tacctaccat tccaatcaat
    56761 gcacgtcgcg taccgcatat tggcgacgtg gtactggcga tcggtaaccc gtacaacctc
    56821 gggcagacca ttacccaggg gattattagt gccacgggtc gaattggtct gaacccgacc
    56881 gggcggcaaa acttcctgca aaccgatgct tccattaacc acggtaactc tggcggcgcg
    56941 ctggtgaact cgctgggcga actgatgggc attaacacgc tgtcgtttga taagagtaac
    57001 gatggcgaaa cgccggaagg tatcggcttt gcgattcctt tccagttagc aaccaaaatt
    57061 atggataagc tgatccgcga tggtcgcgtg atccgcggct acattggcat tggcggacgt
    57121 gagatcgcac cgctgcacgc acagggcggt ggtatagatc aactgcaagg gatcgtggtt
    57181 aatgaagtgt cacctgacgg cccggcggcg aatgcgggta ttcaggtcaa cgatctgatt
    57241 atttcggtgg ataacaaacc ggccatctct gctctggaga cgatggatca ggtggcggaa
    57301 attcgccctg gttcggtgat cccggttgta gtgatgcgtg atgataagca gttaacgctg
    57361 caggtcacca ttcaggaata tccggcaacc aattaagtcg agtgctcaaa acaaaaaacc
    57421 ggagtctgtg
//
LOCUS       DAJVRF010000009        49689 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJVRF010000009 DAJVRF010000000
VERSION     DAJVRF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49689
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.009"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..445
                     /locus_tag="OQN13_000504"
     CDS             <1..445
                     /locus_tag="OQN13_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCS1613966.1"
                     /translation="LVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGAYDGT
                     VFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLEVACARKPDHKRESLAR
                     KIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFMTARGYH"
     gene            complement(494..1993)
                     /gene="pitB"
                     /locus_tag="OQN13_000505"
     CDS             complement(494..1993)
                     /gene="pitB"
                     /locus_tag="OQN13_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCS1613967.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(2285..4144)
                     /gene="gss"
                     /locus_tag="OQN13_000506"
     CDS             complement(2285..4144)
                     /gene="gss"
                     /locus_tag="OQN13_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCS1613968.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            4349..5215
                     /gene="yghU"
                     /locus_tag="OQN13_000507"
     CDS             4349..5215
                     /gene="yghU"
                     /locus_tag="OQN13_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCS1613969.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(5338..5586)
                     /gene="hybG"
                     /locus_tag="OQN13_000508"
     CDS             complement(5338..5586)
                     /gene="hybG"
                     /locus_tag="OQN13_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCS1613970.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(5599..5940)
                     /gene="hypA"
                     /locus_tag="OQN13_000509"
     CDS             complement(5599..5940)
                     /gene="hypA"
                     /locus_tag="OQN13_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS1613971.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(5933..6421)
                     /gene="hybE"
                     /locus_tag="OQN13_000510"
     CDS             complement(5933..6421)
                     /gene="hybE"
                     /locus_tag="OQN13_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCS1613972.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(6414..6908)
                     /locus_tag="OQN13_000511"
     CDS             complement(6414..6908)
                     /locus_tag="OQN13_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCS1613973.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(6908..8611)
                     /gene="hybC"
                     /locus_tag="OQN13_000512"
     CDS             complement(6908..8611)
                     /gene="hybC"
                     /locus_tag="OQN13_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCS1613974.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(8608..9786)
                     /gene="hybB"
                     /locus_tag="OQN13_000513"
     CDS             complement(8608..9786)
                     /gene="hybB"
                     /locus_tag="OQN13_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCS1613975.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(9776..10762)
                     /gene="hybA"
                     /locus_tag="OQN13_000514"
     CDS             complement(9776..10762)
                     /gene="hybA"
                     /locus_tag="OQN13_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCS1613976.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(10765..11883)
                     /gene="hybO"
                     /locus_tag="OQN13_000515"
     CDS             complement(10765..11883)
                     /gene="hybO"
                     /locus_tag="OQN13_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCS1613977.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(12072..12359)
                     /locus_tag="OQN13_000516"
     CDS             complement(12072..12359)
                     /locus_tag="OQN13_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCS1613978.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(12478..13365)
                     /locus_tag="OQN13_000517"
     CDS             complement(12478..13365)
                     /locus_tag="OQN13_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS1613979.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            13522..14562
                     /gene="gpr"
                     /locus_tag="OQN13_000518"
     CDS             13522..14562
                     /gene="gpr"
                     /locus_tag="OQN13_000518"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCS1613980.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRIGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(14602..15096)
                     /locus_tag="OQN13_000519"
     CDS             complement(14602..15096)
                     /locus_tag="OQN13_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCS1613981.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            15287..16171
                     /gene="yghA"
                     /locus_tag="OQN13_000520"
     CDS             15287..16171
                     /gene="yghA"
                     /locus_tag="OQN13_000520"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS1613982.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(16331..16756)
                     /gene="exbD"
                     /locus_tag="OQN13_000521"
     CDS             complement(16331..16756)
                     /gene="exbD"
                     /locus_tag="OQN13_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCS1613983.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(16763..17497)
                     /gene="exbB"
                     /locus_tag="OQN13_000522"
     CDS             complement(16763..17497)
                     /gene="exbB"
                     /locus_tag="OQN13_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS1613984.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            17749..18936
                     /gene="metC"
                     /locus_tag="OQN13_000523"
     CDS             17749..18936
                     /gene="metC"
                     /locus_tag="OQN13_000523"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCS1613985.1"
                     /translation="MTDKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDCVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            19076..19735
                     /locus_tag="OQN13_000524"
     CDS             19076..19735
                     /locus_tag="OQN13_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1613986.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(19775..20674)
                     /gene="yqhC"
                     /locus_tag="OQN13_000525"
     CDS             complement(19775..20674)
                     /gene="yqhC"
                     /locus_tag="OQN13_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCS1613987.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            20868..22031
                     /gene="yqhD"
                     /locus_tag="OQN13_000526"
     CDS             20868..22031
                     /gene="yqhD"
                     /locus_tag="OQN13_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCS1613988.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANDVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            22136..22963
                     /gene="dkgA"
                     /locus_tag="OQN13_000527"
     CDS             22136..22963
                     /gene="dkgA"
                     /locus_tag="OQN13_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCS1613989.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            23163..24089
                     /locus_tag="OQN13_000528"
     CDS             23163..24089
                     /locus_tag="OQN13_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCS1613990.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            24140..24397
                     /gene="yqhH"
                     /locus_tag="OQN13_000529"
     CDS             24140..24397
                     /gene="yqhH"
                     /locus_tag="OQN13_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCS1613991.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(24440..26659)
                     /locus_tag="OQN13_000530"
     CDS             complement(24440..26659)
                     /locus_tag="OQN13_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCS1613992.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSPRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLHCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(26770..28182)
                     /gene="ftsP"
                     /locus_tag="OQN13_000531"
     CDS             complement(26770..28182)
                     /gene="ftsP"
                     /locus_tag="OQN13_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCS1613993.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(28257..28994)
                     /gene="plsC"
                     /locus_tag="OQN13_000532"
                     /gene_synonym="parF"
     CDS             complement(28257..28994)
                     /gene="plsC"
                     /locus_tag="OQN13_000532"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCS1613994.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(29227..31485)
                     /gene="parC"
                     /locus_tag="OQN13_000533"
     CDS             complement(29227..31485)
                     /gene="parC"
                     /locus_tag="OQN13_000533"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCS1613995.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDIACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKVAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(32031..32513)
                     /locus_tag="OQN13_000534"
     CDS             complement(32031..32513)
                     /locus_tag="OQN13_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1613996.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(32566..32958)
                     /gene="ygiW"
                     /locus_tag="OQN13_000535"
     CDS             complement(32566..32958)
                     /gene="ygiW"
                     /locus_tag="OQN13_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCS1613997.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            33110..33769
                     /gene="qseB"
                     /locus_tag="OQN13_000536"
     CDS             33110..33769
                     /gene="qseB"
                     /locus_tag="OQN13_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCS1613998.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            33766..35115
                     /gene="qseC"
                     /locus_tag="OQN13_000537"
     CDS             33766..35115
                     /gene="qseC"
                     /locus_tag="OQN13_000537"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCS1613999.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            35225..35806
                     /gene="mdaB"
                     /locus_tag="OQN13_000538"
     CDS             35225..35806
                     /gene="mdaB"
                     /locus_tag="OQN13_000538"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCS1614000.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            35837..36151
                     /locus_tag="OQN13_000539"
     CDS             35837..36151
                     /locus_tag="OQN13_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCS1614001.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(36199..38091)
                     /gene="parE"
                     /locus_tag="OQN13_000540"
     CDS             complement(36199..38091)
                     /gene="parE"
                     /locus_tag="OQN13_000540"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCS1614002.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPNSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(38120..38701)
                     /gene="yqiA"
                     /locus_tag="OQN13_000541"
     CDS             complement(38120..38701)
                     /gene="yqiA"
                     /locus_tag="OQN13_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCS1614003.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(38701..39528)
                     /gene="cpdA"
                     /locus_tag="OQN13_000542"
     CDS             complement(38701..39528)
                     /gene="cpdA"
                     /locus_tag="OQN13_000542"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCS1614004.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(39553..39975)
                     /locus_tag="OQN13_000543"
     CDS             complement(39553..39975)
                     /locus_tag="OQN13_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCS1614005.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(39976..40605)
                     /gene="nudF"
                     /locus_tag="OQN13_000544"
     CDS             complement(39976..40605)
                     /gene="nudF"
                     /locus_tag="OQN13_000544"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCS1614006.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            40810..42291
                     /gene="tolC"
                     /locus_tag="OQN13_000545"
     CDS             40810..42291
                     /gene="tolC"
                     /locus_tag="OQN13_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCS1614007.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            42439..43110
                     /locus_tag="OQN13_000546"
     CDS             42439..43110
                     /locus_tag="OQN13_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCS1614008.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            43116..44276
                     /locus_tag="OQN13_000547"
     CDS             43116..44276
                     /locus_tag="OQN13_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS1614009.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(44314..45102)
                     /gene="ygiD"
                     /locus_tag="OQN13_000548"
     CDS             complement(44314..45102)
                     /gene="ygiD"
                     /locus_tag="OQN13_000548"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCS1614010.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            45245..46018
                     /gene="zupT"
                     /locus_tag="OQN13_000549"
     CDS             45245..46018
                     /gene="zupT"
                     /locus_tag="OQN13_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCS1614011.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(46076..46246)
                     /locus_tag="OQN13_000550"
     CDS             complement(46076..46246)
                     /locus_tag="OQN13_000550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614012.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(46507..47160)
                     /gene="ribB"
                     /locus_tag="OQN13_000551"
     CDS             complement(46507..47160)
                     /gene="ribB"
                     /locus_tag="OQN13_000551"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCS1614013.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(47264..47412)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            47534..47824
                     /gene="ubiK"
                     /locus_tag="OQN13_000552"
     CDS             47534..47824
                     /gene="ubiK"
                     /locus_tag="OQN13_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCS1614014.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            48107..48658
                     /locus_tag="OQN13_000553"
     CDS             48107..48658
                     /locus_tag="OQN13_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCS1614015.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            48718..>49689
                     /locus_tag="OQN13_000554"
     CDS             48718..>49689
                     /locus_tag="OQN13_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1614016.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQ"
BASE COUNT        12447 a        13181 c        12326 g        11735 t
ORIGIN      
        1 gctggtaatt acagcgtttg tcgcccgtcg cttactgcgc tttcgtcata tgcttgcctg
       61 tcgtcgtcgc gggttaattg ttctaaccga ccgttatcct caggaccaaa ttcctggcgc
      121 ttacgatggt acggtgttcc cacctaacgt tgaaggtggt cgttttgtct catggctggc
      181 aagccaggaa cgtaaagcgt ttcactggat ggcgagccat aagcctgatc tggtcatcaa
      241 actcaatgtt gaccttgaag ttgcctgtgc acgtaaaccc gaccataaac gggaatcgct
      301 ggcgaggaag attgccataa cgccacagtt aacctttggt ggtgcacaac tggttgatat
      361 cgatgccaat cagccactgg aacaggtgtt ggttgatgca gaaaaagcga ttacggattt
      421 tatgaccgcg cgtggttatc actagtcaaa aatggaaatg cccgatcgcc aggaccgggc
      481 attttcagga aggttaaatc aactgcaatg ctatccagta cagcccacca gaaagaaaaa
      541 ttgccgccgg taaagtaaac acccacgcca tcaggatgct ggttaccgtt ttacgctgta
      601 acccaccgcc gtccaccacc atcgtccctg ctaccgcaga cgagaggacg tgtgttgtgg
      661 agacaggcat cccaatataa ctggcaaggc cgatagacac tgccgccgtc atttgtgccg
      721 ccatgccttg cgcatacgtc atgccgcgct taccaatctt ctcaccgatg gtcatcgcta
      781 cacgacgcca gccaatcatg gtgccaatgc cgagcgccag tgctaccgcc atgatgatcc
      841 acaccggagc gtactcaatg gtgcttaaca tatcgctgcg cagttttttc agcaggttct
      901 ggtcttcttt actgacgcct ggcagtttcg ccagcttcgc ggaggtatca gagatgcaca
      961 gcataatgcg gcgcagctgg ctgcgctgac tcacgcttaa cggctcgtaa ctttccatat
     1021 tgcctggcag catcgtttta acgcgcgcaa tcgcatcaaa ggtatttgcc ggatgacagt
     1081 gaaactctgt tacttgcgtg ccatcagtcg atgctgcagg caatggaggt tccatcgcaa
     1141 tcaacttctg cggcagttca ggatgctgtt gcaggtagtg ttcgaagttg gtgacggcat
     1201 cgcgggtacg ggtaatttca tagccggacg cgttcatatt gacgacgaag ccagcagggg
     1261 caatccccac cagtaccagc atcaccaggc cgatcccttt ttgtccgtcg ttcgcgccgt
     1321 gcgaaaacgc cacgcccgca gcggaaacaa tcagcgcaat acgcgtccag aatggcggtt
     1381 tacgtttgcc ttttttcttt ttgcgatctt ccggaatgcg gtgaatacgg tcacgctttt
     1441 tcgtcccgct ccagtagcgt cgcagcagga atatcaggcc tcccgcaatg accaggccga
     1501 cgatagggga aacaatcagc gaggagaaaa ttttggtcac ttcacgcagg tttaacgcat
     1561 ccatcaccga tgagccggtt aacagcgcgt tggttaaacc gatgccgata atcgcaccaa
     1621 tcaaggtgtg cgaactggag gccggtaaac cgaagaacca cgttcccagg ttccagataa
     1681 tcgccgccag cagcatggaa aagaccatcg ccaggccgtg ggttgacccc atattcagca
     1741 acaaatcggt tggcaacata tggacaatgg cataggcaac gctaagtccg cccaataaca
     1801 cgccaaaaaa gttaaaaaat gccgccatca ccacagcaag ttgtggttgc atggcacgag
     1861 tataaataac ggttgccacc gcattcgccg tgtcatgaaa accattgatt gcttcgtaga
     1921 acaacacaaa tgccagagca agcaataaca aaagccctgt gtatatatca aggccaacaa
     1981 ataaatttag catatattag attacgccat tttgaattta cgaacggacg cattatcagt
     2041 gactttaacg gcatgggcaa agtgaaatat cattttttaa cgataaaaag agtgttattt
     2101 cttgtgtatc agacaataag cattcattca aaacctttag aaataatcaa aacacaggta
     2161 ttccactggt taatatattc acatatgaaa taaataaata ttagaacata taaatattga
     2221 atattttgat taatccccgt actgattatt cttcataatc agtacggggt tgctacaaca
     2281 tgtattactt tttcaccaca attaacggtt caatatcact ctcttttttg atgaccagtg
     2341 attcatcacc gcgcaaacac gtcccaccgt agttgccgcc cacggtgaag gtacatacct
     2401 gaatgtattt accgtccact ttcggcaaac accacagttg ctgatagatg tttttctgct
     2461 cggcaaattt accgctggtt ttgtccagca cctcttcatg atggctgacc aggtcgatat
     2521 tgctaccaca gcgaccggca atcggtttca ctgcgtaacc tgttttcacc aattcatcat
     2581 taacagtgaa atcggtatcc agcaggtaac gatggtgcgg gaacagcgac cagaggatcg
     2641 gcagaatcgc tttgttgccg gggatcaccg tccacagcgg ctcaaagact agcacttccg
     2701 ggcgcagcaa tacgtcgata agacgcactt cgttttgcgg atgaccggta cggattggca
     2761 ccgcagcaaa ctcacggtcg ctaacttcac gaatctgatc aaacgcggtt tcccacgccc
     2821 aggttttcca cacgcagtta accagtcgcc cttccccatc aatcagttgc ccggcagcat
     2881 cccagcccag ttcatccaat ccacgcaaga tacgcgtttc aaagcccgcc tggtgcagcg
     2941 cctgctccat aaactgcgcg tgatagtttt cctcgatatc tttgtcctgc atgatatgga
     3001 caaacggacg tgcacgactg tgtttccagg caccagccaa ttcgttaatc agcccttccg
     3061 ccggattgaa gccgttgcct ttatagccct gctccgccca acgttcgagg atcaagcccg
     3121 cttcggtatg acaggaggcg gagtcggcgt tgtactcgta aaccttcagg ccacgctcat
     3181 ccatgcagaa atccatacga ccagtgatca tatggtgacg gcgacgctgc caggagagac
     3241 gcaaacgtgg ccagaggatt ttcgggatgt cgaacagcgc cagcaggttg tcatctttca
     3301 gcaccttgtc ggttgcgtga agatacatca ggtgcagctc gttggtggct ttaattagct
     3361 cctgctcggc actctcggta atggtgtagt aatgataagg atcctgattg atcacctgac
     3421 cgttggcctg cacataggcg ttttgcagcg gatctttttc atccagccat ttaccgtcaa
     3481 actggccttt gttttccagg cgcgctccgc tgattttcag cagctcgcct gcaatttccg
     3541 gctgcggtaa gctgtattca gtatcttccg tctggatcat ccagcccaga atggtggtgt
     3601 catcaaaagt gtctttcagg gtatagcagc cgttttcgac caccatctcc agctcacgcg
     3661 tccactgttg cccttgcggc aacggggaat gaatcacgtt ctgttccgca atacggactt
     3721 tgttgccatg caattgggta atgatggcga catggccagt gtctttaaat tcaccgcctt
     3781 tatcccagat aagaagcgca cccgcgaccg gcgcacgcgg cgagccgtta ggaaatgcct
     3841 gcaatggcag gatgttgtca ttaaccactt ctcgcaggaa gcgcagcgag aaaatctccc
     3901 acgccatacc cacgtcagta aagaccacac cgtaattcag aaagagaaaa cggcgagcaa
     3961 attcaacgca ttgccacttg tggcccatat attcgtcgtc gatatagcta cggaatacgg
     4021 cgtcatcttc gtattcctgc ggatcgagag aactgtaatc tgaagagtag attgctaccc
     4081 cacctggggc gtagcccaat aatgtcccga acggggcatc ctggctggtc gttcctttgc
     4141 tcatcacttt acctttaaca atacaaccta agcagttggc gtaattgttg tgctctgatt
     4201 acctgctcag cgacattaac cggacagagg tcagactaat catacacctc atcgcagcgg
     4261 ctggcgcaaa gggttaaaaa attcacattc tgtacagcaa gtgacctgct acactgcttc
     4321 aacactacca ctcagaaggc aactcactat gacagacaat acttatcagc ccgcgaaagt
     4381 ctggacgtgg gataaatccg ctggcggcgc gttcgccaat atcaatcgcc cggtttctgg
     4441 tccgacgcat gaaaaaacgc tgcccgttgg caaacaccca ttgcaacttt attcgctggg
     4501 aacgccgaac ggtcagaaag taacgattat gcttgaggag ctgctggcgc tgggcgttac
     4561 tggtgcagag tacgacgcct ggctgattcg tattggcgat ggcgatcaat tctccagcgg
     4621 ctttgtcgaa gtgaacccaa actcgaagat cccggcgctg cgcgatcata cgcataatcc
     4681 gccgatccgc gtgtttgaat ctggttcgat cctgctttat ctggcggaga aatttggcta
     4741 cttcctgccg caggatttgg caaagcgtac tgaaacgatg aactggctgt tctggttaca
     4801 gggcgcggca ccgttcctcg gcggtggttt tggtcacttt taccattacg cgccgatgaa
     4861 aattgagtac gccatcaacc gctttaccat ggaagccaaa cgtctgctcg acgtgctgga
     4921 taagcaactg gcgcagcata agtttgttgc gggcgatgag tacaccattg cggatatggc
     4981 gatttggccg tggtttggca atgtggtgtt aggcggcgtg tatgatgccg cagagtttct
     5041 tgatgcgggc agttataagc atgtacaacg ctgggcgaaa gaagtaggcg aacgtccggc
     5101 ggtgaaacgt gggcgtattg ttaaccgcac caacggaccg ctgaatgagc agttgcatga
     5161 gcgccatgac gccagtgatt tcgagacgaa tacggaagat aagcgtcagg ggtaagggtt
     5221 ggtgttcgtc gcagcaagcc atccaggccg gataaggcgt tcacgccgca tccggcaatc
     5281 gtgcacaatg cctgatgcga cgctgccgcg tcttatcagg cctacgaaag ctaatcatca
     5341 cgcgctggta atgtcgtaat ccatttggcg cagtgcgtct aatgtggctt tggcttcatc
     5401 ttcgtcgatg atgctcatgg caaatccgac gtgtaccagc acccactggc ccagtagatc
     5461 ggcagggtta ccttcacaaa tcagggcgat attcacgtcg cgcttgatac cacatacttc
     5521 aacctgcgca agctggtgaa tatcttcacc gacagccagc acctggcctg gaacgccaat
     5581 acacattatt aactccggtt attcaacttc aatacttttg acgatcagcg aatcgccggt
     5641 atcgacacgc aaccgctcgc cgtgacagag cggacactgc gcatcgtgct gatgaatctc
     5701 caccacctgg ctgcaatccc agcaccaagc ctgggcgggt ttatagacga tatgtaaatc
     5761 gcacccttgc gccaccgttc cgtggcagac aatttcaaaa ctaaaacgga cggcgctctc
     5821 ctcaacgcag gagagcgcgc caatttccag ccacacggcg gtgacgcgct taacatcgtg
     5881 ctgctccgcc tgccgttgga taatttcaac ggcgctctgg caaagagaca actcatgcat
     5941 tttcgccact cctgcgacca aacagcaggg cgcgacgccc tgcgtgtggt acatccggat
     6001 tcgtgactgg cagcgaaagg atcattcgtg cgcagtcatc cgtcaggcgt tggccctctt
     6061 caatcgacat gctgtgcgaa agcggcgaca tcagcgaaca ggagagatat tgcgaaacac
     6121 cgtccagttc gccaacggta aaggtcatag tgccatacgg cagttgcaga ccaatttttt
     6181 cactcacttt gcgcagcggc cagagttgat ccgggccggg gaagataact gcactcagca
     6241 tccacggggt gatcacacac cccgtccact gaccttcgaa cagcgtaaaa tcagaaacat
     6301 acaccggcat tgaaggatgc agaaaagaga gatcgtgcat cgaacgccgg gcaatttctt
     6361 caaacgctgc ctgtacttgc gccttcgggg aggtctggaa acctgctatc tcttcagtca
     6421 tgaatcgcct cccgtgggat ggcttccacg cctgattcgc gcagcgcagc cagaacctgc
     6481 tcaagcgcag gttcaatcat tgcttcaacc gtcggcgtta agccgatgtg tggctccagc
     6541 gattccggga tcacgccgac cagggtcagc tttttcggaa actcaccggt gaagcgcagg
     6601 gccgacaata cgtcggccag gccaagctga tgcggagaga ttttgttggt aaacaacgcc
     6661 ggaacttctt catcccgcag gatcatcatc gttcccggcg tgttcttttt cgacacaatg
     6721 gcatccgcaa taatcagatg atcgcgattt gccatgtcgc caagcagctc cattcccgcc
     6781 gtgccgccat cgaggatctc aacataatcc ggcagaatgt atcgttgctc taacgcttcg
     6841 acaatccgca caccgatggc ttcatcggtc agcaaaatat tgccgacccc taagactaaa
     6901 atacgcatta cagaaccttc actgaaacca cttcgttgcc gtcggcatcc actacgtgta
     6961 ccgcacaggc catgcacggg tcgaaggagt gaatggtacg caccacttcc agcggtttat
     7021 tcggatcagc aaccggtgta cccaccagcg actgctcgta aggaccgacg tcatcattga
     7081 agttacgcgg accagagttc caggttgatg gaacaactgc ctggtagtta ctgataatac
     7141 cgtctttaat aaccatccag tgagagagca taccgcgcgg cgcttcgagg aagccaacac
     7201 ctttgaactc acccgttgcc ggaatgttcg gtttcacaaa ggtggtgtga tcgcctttgc
     7261 cgatattggt gatcagtgca ctgtattgat tttgcaggat atcctgcaat tcgcagcagt
     7321 gaacggtacg accaataata cggcccagcg tggagtgcag ctgcgccact tccagcgtgt
     7381 tgccagtcag tttctgataa atcgcaacga tttcattcag tttgttctgg gtagattcgc
     7441 gacctgccgc cagtttcacc agcatattag ccagcggacc cacttctacc gttttgccgt
     7501 agaaagtcgg tgatttcacc caggaatatt tcccgtcgtc agaccaacca tcataagccg
     7561 gaatggtggt accttcccac ggtgcctgcg gcgcttcgtc tttataccag gagtgcttcg
     7621 cgctttcctg aatgcctttg atcaggtatt catcggaatg agaagtgatc ggacgatacg
     7681 aggacagatc cgcattctca atgtagccgc ccgggaacag gaagctgccg tttttactgt
     7741 cggtcgggaa ttccggcacg ctcaggtagt tcaccgcacc tttaccgcgc gtcagccatt
     7801 ccgggtagaa cgcggcaata actgcggtat caaccttata aacctgttca acaaagtcgc
     7861 tcagcttgtc gatgaaagac ttgatgtaca tcaggcgctc aaggttcagc acgcccaaac
     7921 cgtcgaggtt gatcgggttc gcgacaccac ctaccgccag gttctgaatg tgcggcgttt
     7981 taccgcccag cagcgccacg acgcggttag cgtcacgctg gcactccagc gcctgcaggt
     8041 agtgcgctac cgcaatcagg ttcacttccg gcggcagttt catcgccggg tgaccccagt
     8101 agccattggc gaaaataccc aactgaccgc tggcaaccag atctttgatc ttgttctgaa
     8161 ctttagtgaa ctcttccgga ctgttcagat gccaggtcga aacgcctttc agcatttcgg
     8221 aggctttggt tgggtcagct tgcagtgcag aagtgatgtc cacccagtcc agcgccgaaa
     8281 gctgatagaa atgaacgata tggtcatgcg tggtgtgcgc agccagaatg atgttacgga
     8341 tgtattgcgc gttaaccgga acgtcgatat tcagcgcact ttctgccgca cgaacggaag
     8401 acagcgcgtg agtggtagta catacgccac agatacgttg cacaatcatc catgcatcgc
     8461 gcggatcgcg gtttttcacg atctcttcca tgccgcgcca catggtaccg gaagcccatg
     8521 ctttcgaaac gacgccattt tcgatttcgc aatcgatgcg taaatgcccc tcaatacggg
     8581 ttaccggatc aatagtaatt ctctggctca tgctttgctc gcctcatgac gattatgatc
     8641 gttttgtttt aaaggaggaa gtatcggcag tagacgaatg agtacgatgt aagcgcaaat
     8701 ctcaatagcc acaaaaccaa tagaaatcaa cagttcttcc caggtcggga agtaggcgta
     8761 accgccgccc gggttgaatg ccaccagcga ataggtcaga cgccaggttg cacaacctaa
     8821 cagtgcgctc agtgctgaca ggaacagcat gcgggaatca ttacgcagct tcgccacacg
     8881 cagaacgacc agcgggaaga gcatcagcag gacttcaatc cagaacatca cggagtagaa
     8941 gtcaccggca aacgctaacg acagcttgtc gcgatagata agctcgccaa agcgcagcac
     9001 gatgaaaatc gccagcaaca cactgatggt gttggtcagc ttgacgaaca gactcttttc
     9061 atccggaccg ttgccacgca gacccgcctg caccagcgaa ccttcaaaga tgacaatcga
     9121 gaagcccatg atgaacgccg tcagcagcga gaacagcggc aacatttcat agctctgcca
     9181 caacggatgc accttgtagc ccgccgagat catcagtgac cccattgaag actggtgcat
     9241 ggtcggcaac agcgcgccaa gtgcaatgat gaagaacata accttgttca gacgcttgag
     9301 agaaaccttc cagcccagac gttcaaacag tgccggagca aactccagtg ccatcacgcc
     9361 gatatagatg gtcatacaga ccgccgtctc gaacagtacc gagttcacgt tgaagtgacc
     9421 cggaatgtag aagtacggca ggttccagta gcgacccacg tcgatagtga tcgacaagcc
     9481 acccagtgag taaccaaaca gactcgccaa cagcgccgga cgcaccagcg gatggtattg
     9541 cccacggtta aagacgtata ccgcccacgc cagcgcccag ccgccacagg caaagccggt
     9601 gccaatcagc aggtcaaacg cgatccacac gccccacggg aagccgccgt tcaggtcaga
     9661 gacagagccc agaccgaaca ccagacgctt cacaatcagg agcatacaga tgacgattaa
     9721 cggtccaaaa atcatgaccg gtttactgat gattttgccg cccagcggtt gtggatcatg
     9781 actcatgatc gtctcctccg tcgtgatggt cgtttttggt gttgcgacga accagcacgg
     9841 ttaagcccgc cagcacagcc agtggtagca tcatgccttt atacagggtg tgttgaatat
     9901 tttcggaacg cgcaccggta gaaagatcgt ccagtttcgg caggtcgaga ttttcataag
     9961 gcacacccgt cagtaccaga acctgagtac cgccgccctc tttctcgccg tacagatgcg
    10021 gataatattt cggcaccgta tgcaggtaag tgtcgccaga tttcagcgtc tgacgcggat
    10081 agtggtattc gctgccaggc ttcagcgcca gacgtttttt cgcctccgcc atcagctctt
    10141 cacgcgtacc gaaaatcacc gcgcccgccg ggcacacttc tacgcagcca ggcagaccgc
    10201 ctttatcgag acgttccaca cctttctggt tgcacagctc gcacttatgc agcgcaccaa
    10261 acgggttgtt gtagtcgtac ttcggcacgt tgtacggaca ggcgaccatg cagtaacggc
    10321 aaccggtgca cacgtctttg tcgtaatgga caatgccggt tttcggatct tttttcagtg
    10381 cagagaccgg gcacacagag acacagttcg gatcgacgca gtgcatacac tgtttcttaa
    10441 tgtacgcgta gccgttctcc tcctggtctt tgttgacccc tgtgccgctg gtccacacct
    10501 gaatgatgtt attggtatac ggcgacagtt tgtcgttgtt cgaccaggtc tgttcccctt
    10561 gcgggttacg ttcagggaag ttgatatcct gacacttggt gacgcaagcc tggcagccta
    10621 cgcacaaggt cgagtcgtac aacatcccca gcgatcccgg aattggcggg cggttttcag
    10681 cagccgcatg actgacagac ggcagcgcgc ccgtcagcaa tgccccgcag gaggctgctt
    10741 taataaaatt acgtctgttc acggttattc tccccgtgag tcagcgttat ctttcttttg
    10801 ctgacgaccc agttcacgca ccgccatcac gctgacaccg gcaaccagcc caaccacacc
    10861 gccgagcaaa ccaatagcgc ctgcagagac gttgccgccc tctttagcgt taacatccgg
    10921 tttctgtgaa cgcggagtct gattttcgac gttggcaagc tgatggatgc ctttatggaa
    10981 gccgatacct tcttcgttac agccatagca tgggtgacca atcgctaccg gccacacgcc
    11041 gccaacatcg cagaattgca gcgttgagca gttgccgtaa gtttctggcc ctttacagcc
    11101 gaggtggtac aggcaccagc cttcacggtg gccttcatca ccgaactctt tggcaaaacg
    11161 accagcatcg aagtgcgggc gacgttcgca gtgttcgtga atcagacggc cataggcgaa
    11221 ggtcggacgg tttttgtcat ccagtttcgg cggtttgccg taagtgatga tgtgcgcaac
    11281 ggtcgcgagg aagttgtgcg ggttcggcgg gcagcccgga atattgataa cggttttgcc
    11341 tggcagaact tcttgcaggc tgactgcgcc agttgggtta actccagctg cggcaacacc
    11401 gccccacgca gagcaggaac cgatagcgat aatcgctgct gcgccttctg ccgctttgcg
    11461 gatgtgatcc acaatcggct caccagcaac catgcaataa ataccgttat cttttaatgg
    11521 gatggaacca tccacaacca acacatactg ccctttgtac ttctcaagtg cgttatgttt
    11581 gttctcttcg acctgatgac cgaaggcggc ggaaagcact tcgtgatact ccagagagat
    11641 agtctccagc acgaggtttt ctaccgttgg atgcgttgca cgaagcagag attccgtaca
    11701 accggtgcac tcctgcgcgc caatccagat aactggcgga cgctgcgggt tagtaaccga
    11761 ttcggccatc tctgcagcgg ctttgctact taaccccatg gtggcggcta atgctgcaca
    11821 aagcttcatg aaatcacgac ggttaatgcc gtgagaatgg atgagggtgt tatctccagt
    11881 catttatagt tattccgttg cgaagacctg gcatatattt tgcctcaatc gcaaaatcaa
    11941 taatgcgatc gatgcgccat ttaccacaca tttattatgg ttatcgatat catgatactg
    12001 cggcggggcg ctaaaacgaa gggaagaagc attagtgtaa ttaattaaag caagataata
    12061 cgtatgtttg atcaattttc gaggcgataa ccggccataa aaaagcgcaa cgtagaacag
    12121 gaattattct cacgaaagaa atccattacc atctctttat ccagtccata gcggcgcgtc
    12181 agaatacccg cttcccaggc gctaagctgg cgatctccgg tcttttcgta catccggtgt
    12241 gagtagccta atacaaaacc gcgtttataa tcggcacaga attttgtcac attaaccgca
    12301 ctgtcggcat gcgttgcttt taatcccgcc attaagcctt taccaaaatg gttattcatc
    12361 gttcacctca ttcgctatat atcgccatat atagcgatat tttaggcaaa gaacgacttt
    12421 tgcgcacgct attttttcat gcttgtttaa gacgtaatcc agtaagagat aaacctatca
    12481 ggagagatat ttatcgaacc atttcagtgt cctttgccag gcaagatcgg cggcagattt
    12541 gtcataacgg ggcgtggaat cattatggaa tccgtgatta acccccggat agatatacgc
    12601 ctcataaacc ttattattgg ctttcaacgc cgcctcgtaa gcaggccagc cctcgttgat
    12661 tcgggtgtcc agttccgcga agtggagtag taaaggcgct tcaatcttcg ccacatcggc
    12721 agtgggtgcc tgacgaccat aaaacggcac cgcgcaggcc agttccggat acgcgacagc
    12781 cgccgcgttc gatacgccac cgccatagca aaatccggta atacccactt tgcctgccgc
    12841 ttgtggatag cgttgcataa actcaattgc ggcaaagaaa tcattcatca gtttggttgg
    12901 atcaacctgc tgttgcagct cacgaccttt atcatcattt cccggataac ctccaacgga
    12961 acttaagccg tcaggtgcca gggcgatata ccccgccttc gccactcgcc gtgccacatc
    13021 ttcgatatac ggattcagtc cacgattctc atgcaccacc accacggctg gcgttttgcc
    13081 gctcattttt gcgggtttca ccagataacc ccgtacctcg ccgtgaccat ttggcgaagg
    13141 atacatgatg tactcagcaa caatctccgg gtcggtaaac tctacctgag tcgccagcgc
    13201 ataatttggc ttgagcaaat caaacaacgc taatgccgtc atcccgccca ccgcatactt
    13261 cgccgcaagg ttgaggaact cacgtttcga aattttcccg tgagcgtaat agtcgtagta
    13321 atccaacaac tcttgtggga aatctttggc ggttaaacgc ggcatcgttg cactcctcag
    13381 ttggtgtttt tttaagcaaa gcataagcac gtgtttttgc ccagtttttc gtcactctgt
    13441 gagccagact acgggatacg cgctggcgaa tcgctaaact agaaacattg tttcgaaatt
    13501 gaacggtgga aaggagaggt catggtctgg ttagcgaatc ccgaacgtta cgggcagatg
    13561 caataccgct attgcggaaa aagcggttta cgcctgcccg cgttatcgct cggtttatgg
    13621 cacaatttcg gtcacgttaa cgcgctggaa tcacagcgtg cgatcctgcg taaagcgttt
    13681 gatttgggca ttacgcactt tgatttagcc aacaattacg ggccgcctcc aggaagcgca
    13741 gaagagaact ttggtcgcct gctgcgggag gattttgccg cttatcgcga tgaactgatt
    13801 atctctacca aggctggcta cgatatgtgg cccggccctt acggctctgg cggttcacgt
    13861 aaatacctgc tcgccagcct cgaccaaagc ctgaagcgta tagggcttga gtatgtcgat
    13921 atcttttact ctcatcgcgt cgatgaaaat acgccgatgg aagaaaccgc ctctgcgctg
    13981 gctcatgcgg tacaaagcgg taaggcgctg tatgtcggga tctcctctta ctcgccagag
    14041 cggacgcaaa aaatggtcga gttgctgcac gagtggaaaa ttccgctttt aattcatcaa
    14101 ccttcgtaca atttactgaa ccgctgggtg gataaaagcg gcctgctgga taccctgcaa
    14161 aataacggcg tgggctgtat tgcctttact cctctggctc agggattgct gaccggaaaa
    14221 tatctcaacg gcattccgca agattcacgg atgcatcgtg aagggaataa agttcgtggt
    14281 ctgacgccga aaatgcttac cgaagccaac ctcaacagcc tgcgcttatt gaatgaaatg
    14341 gcacagcagc gtggacaatc aatggcgcaa atggcgttaa gctggttgct gaaagatgat
    14401 cgcgtgacgt cggtattgat tggtgccagc cgcgcggagc aactagagga gaacgtgcag
    14461 gcgctgaata atctgacatt tagcaccgag gagctggcgc agattgatca gcatatcgcc
    14521 gatggcgagc tgaatctgtg gcaggcgtcg tccgataaat gacctgttaa taacgggcgc
    14581 gcgaaccgcg cccgaatcag atcagtgatt atgacgagtc agtcggtcaa gatagcccat
    14641 cacaaatgca gaaagcacaa acgtcagatg gataatgacg taccacatca gtttgttatc
    14701 cgggacattt ttcgcatcca taaagacgcg cagtaagtga atggaagaaa ttgccacaat
    14761 cgacgctgct actttgtttt tcagcgacgt tgcgtccatt ttccccagcc agttcagctt
    14821 ctctttgttc tcggagatat ccagctgtga gacgaaattc tcataaccgg aaaacatcac
    14881 catcaccagc aaaccgccaa ccagcgtcat atccaccagc gacagcaaca cgaggatcaa
    14941 atctgattcc gccatcgaga agatattcgg cagtacgtga ataatctcct ggaagaactt
    15001 cagcgccagg gcaactaacg ccagcgaaag gccaaagtac acgggggcaa gcagccagcg
    15061 agaagcatac attgcatttt caagaaaacg ttccatacat tcctgtcgtg tagttaaaac
    15121 gggcaacagt atatcgcaat tgagtaaaca gcacgcaaca gcaaatcaac atatacgtta
    15181 aacctttgtc gcataattct gaggtttagg gatcttcctg gttcccttat caacgcatcg
    15241 agagcgtcta cccttaaagg acgttgaacc tgaagggaga aaaacgatgt ctcatttaaa
    15301 agacccgacc acgcagtatt acactggtga atatcccaaa cagaaacaac cgacgccagg
    15361 catccaggcg aagatgacac cggtaccgga ttgcggcgag aaaacctatg ttggtagcgg
    15421 tcgcctgaaa gatcgtaaag cactggtgac agggggcgat tccggaatag gtcgcgctgc
    15481 cgccatcgct tacgcgcgtg aaggggctga cgtggcgatc agttatcttc ccgtggaaga
    15541 agaagacgct caggatgtga aaaagatcat tgaagaatgc ggacgcaaag ccgttctgct
    15601 gccaggcgat ttaagcgatg agaaattcgc ccgttcgctg gttcacgaag cgcacaaggc
    15661 gttaggcggg ctggatatta tggcgctggt cgccgggaaa caggttgcca ttccggatat
    15721 tgcagacctc accagcgaac agtttcaaaa gacctttgcc attaacgttt tcgcgctgtt
    15781 ctggctaacc caggaagcga tccccctgct accgaaaggt gcaagtatca tcaccacttc
    15841 gtcaatccag gcataccagc caagtccgca tttactggac tatgcggcta cgaaggcggc
    15901 gattctgaac tacagccgtg gcctggcaaa acaggtcgcg gagaaaggta ttcgggtgaa
    15961 tattgtcgcg ccaggcccta tctggacggc actgcaaatt tccggcggac aaacgcagga
    16021 taagatcccg cagtttggtc agaaaacgcc gatgaagcgt gcggggcaac cggcggaact
    16081 ggcccctgta tatgtttatc tggcaagtca ggagtcgagc tacgtcaccg cagaagtgca
    16141 cggcgtgtgc ggcggcgagc atttaggtta aaaaaatgcc cggttgtgaa aagcaaccgg
    16201 gcatcattgt gaattcactc ttacctgatg tggcgaaaac gccttatccg gcctacaaac
    16261 gcacgaagat tcaacatgtt gcaatagatt ttgtaggcct gatgagcgta gcgcatcagg
    16321 caattctact ttacttcgct ttggcggttt cttcgccgac cagacctatc ttcaggtaac
    16381 ccgcctgatg cagcgtatcc attaccttca tcaacgtctc gtaatcgacg gttttgtccg
    16441 ctcggaagaa gatggtggtg tctttcttgc cttcggttaa cgcattcaac gccgtaatca
    16501 ttgtttcatc ggtgaccgga tcgttaccga taaacatcga gttgtctgcc ttcaccgaca
    16561 gataaaccgg tttttccggg cgcggctgcg gcgtgctggt agaagcaggc aagttcacct
    16621 tcacatctac cgtcgctaac ggtgccgcca ccataaagat aatcagcaga accaacatca
    16681 cgtcgataaa cggcgtcacg ttgatatcat gcatttcgcc gttatcgtcg aggttttcgt
    16741 taagatgcat tgccatcgga cattatcctg cgcgtaattt ttgtgcgaca cgaaccggat
    16801 gcgcagcggc gctggcttcc agatccaggt cacggctttg cagcaacaat acctgcgctg
    16861 caacatcacc cagcatcgct ttaaagccgc caatctggcg tgcaaatacg ttatagataa
    16921 cgaccgcagg aatcgctgcc acgaggccga ttgccgttgc taacagagct tctgcgatac
    16981 ccggcgcaac gactgccagg ttagtggtct gcgtttgcgc gataccaata aagctgttca
    17041 tgatgcccca gaccgtacca aacagaccaa cgaacggaga aatcgcgccg atggttgcca
    17101 gatagccgtt accgcggccc atttgacgac ccactgcggc gacccgacgc tccagacgga
    17161 agctggtacg ttctttaata ccttcgttat cgtcgctgcc ttctgacagt tccagctcat
    17221 tctgcgcttc attgagcaaa tgcaggctta agcttttgct accaaaatca gcggcgatat
    17281 cgttggcctg gtttaaggaa cgcgcttcag ccagcagttg ctgctcgcgc ttaaggcgac
    17341 gcttctgatt gaagaactct acgctcttac tgaagaagat tgcccaagtg actacggagg
    17401 ccaaaataag cccaatcatc acgcacttaa cgacaatatc ggcgtgctga tacatacccc
    17461 agacggaaag gtccgtctgc atcaaattat tacccactgt gtatctccag gacgcaagtc
    17521 acaaaatctg cgcataataa tatcaaaatg acgtcgaatt gatagtcgtt ctcattacta
    17581 tttgcatact gccgtacctt tgctttcttt tccttgcgtt tacgcagtaa aaaagtcacc
    17641 agcacgccat ttgcgaaaat tttctgcttt atgccaattc ttcaggatgc gcccgcgaat
    17701 attcatgcta gtttagacat ccagacgtat aaaaacagga atcccgagat gacggacaaa
    17761 aagcttgata ctcaactggt gaatgcagga cgcagcaaaa aatacactct cggcgcggta
    17821 aatagcgtga ttcagcgcgc ttcttcgctg gtctttgact gtgtggaagc caaaaagcac
    17881 gcgacgcgca atcgcgccaa tggcgagttg ttctatggac ggcgcggaac gttaacccat
    17941 ttctccttac aacaagcgat gtgtgaactg gaaggtggcg caggctgcgt gctatttccc
    18001 tgcggggcgg cagcggttgc taattccatt cttgcttttg tcgaacaggg cgatcatgtg
    18061 ttgatgacca acaccgccta tgaaccgagt caggatttct gtagcaaaat cctcagcaaa
    18121 ctgggcgtaa cgacatcgtg gtttgatccg ctgattggtg ccgatatcgt taagcatctg
    18181 cagccaaaca ctaaaatcgt gtttctggaa tcgccaggct ccatcaccat ggaagtccac
    18241 gacgttccgg cgattgttgc cgccgtacgc agtgtggtgc cggatgccat cattatgatc
    18301 gacaacacct gggcagccgg tgtgctgttt aaggcgctgg actttggcat cgatgtttct
    18361 attcaagccg ccaccaaata tctggttggg cattcagatg cgatgattgg cactgccgta
    18421 tgcaatgccc gttgctggga gcagctacgg gaaaacgcct atctgatggg ccagatggtc
    18481 gatgccgata ccgcctatat aaccagccgt ggcctgcgca cattaggtgt gcgtttgcgt
    18541 caacatcatg aaagcagtct gaaagtggct gaatggctgg ctgaacatcc gcaagtagcg
    18601 cgagttaacc accctgctct gcctggcagt aaaggccacg aattctggaa acgagacttt
    18661 acaggcagca gcgggctatt ttcctttgtg cttaagaaaa aactcagtaa tgaagagctg
    18721 gcgaactatc tggataactt cagtttattc agcatggcct actcgtgggg cgggtatgaa
    18781 tcgttgatcc tggcgaatca accagaacat atcgccgcca ttcgcccaca aggcgagatc
    18841 gattttagcg ggaccttgat tcgcctgcat attggtctgg aagatgtcga cgatctgatt
    18901 gccgatctgg acgccggttt tgcgcgaatt gtataacatt gccacttttg gacaattttg
    18961 cagacattta attgtgaaaa gtcttaaatt gttgcgtccg ggatcaaggc gtcccggacg
    19021 aatcaggagt acaataggca gataaaggct taaacgctgt tccacaggaa agtccatggc
    19081 tgttattcaa gatatcatcg ctgcgctctg gcaacacgac tttgccgcgc tggcggatcc
    19141 tcatattgtt agcgttgttt actttgtcat gtttgccacg ctgtttttag aaaacggcct
    19201 gctgcccgcc tcatttttgc caggcgacag cttgttgata ctggcaggcg cattgattgc
    19261 ccaaggggtt atggattttc tgcctacgat tgcgattctg accgccgcag caagtctggg
    19321 ctgctggcta agttatattc aggggcgctg gttagggaat accaaaacgg tgaaaggctg
    19381 gctggcacag cttcctgcta aatatcacca gcgcgccacc tgcatgtttg accgccacgg
    19441 tctgctggcg ctgctggctg gacgttttct tgcatttgtc cgtacgctgc tgccaaccat
    19501 ggcgggaatt tccggtctgc caaaccgccg cttccagttt ttcaactggt taagtggatt
    19561 gctgtgggtc agcgtggtaa ccagttttgg ctatgcctta agtatgattc cgttcgttaa
    19621 acgccatgaa gatcaggtaa tgacgttcct gatgatcctg ccaattgcct tgttaaccgc
    19681 tggcttgtta ggcacgctgt ttgtagtgat taaaaaaaaa tactgtaacg cctgacgatt
    19741 ttccccgttc ccggctgctg taccgggaac gtatttaatt cccctgcatc gcccgcattc
    19801 ttgccgcatc ttcccccggc gtcacaccga agtaacgttt aaactcacgg ctaaattgcg
    19861 atgcgctttc atagtcgacg cgcatcgctg ctgcgctggc cttcatgccg tcatggatga
    19921 tcatcatccg cgccttatgc agacggtaat tcttcaaata ctgcaacggc gaggtgctgg
    19981 tgacagactt aaaattatga tggaacgccg atacgctcat gttggcttct gccgccagtt
    20041 gctcgacgct caggttttcg gtgtatttat tctcaatccg tttcagcacg cggctaatca
    20101 gactgaagtg agtctggcga ctgaccagcg ccagtaacgc gccgccgcaa ggtccggtca
    20161 gcacgtagta cagaatttcg cggatgatct gtttgccgag aatacgcgca tccagtggtc
    20221 gctccatcac gtcgagtaac cgctccgccg cgcataaaat ctcttctgat aacgtggcgg
    20281 agttaatccc gctggctgcc atcgacggct ggaaatgctc atcttcgcca atgtccatca
    20341 acagttcctg taactgcaaa atatcgacat tgagacgcaa tcctgccagc ggcacctctg
    20401 acgtggcata ggtttcgcac tcaaacggca acggcaccgt cagcagcagg tattcattgg
    20461 catcataacg aaacacgcgt tcattgatat aaccgatttt atgcccggaa aagagaatta
    20521 tgatgccagg ctcgtacatc accggtgtac gtgcgaaagg cgtctcgcca tacaacaaac
    20581 gcacatcggg caacagttct gacaaactat tttctttatt tttcagttta ttaactttat
    20641 ccgccagcaa gcggcaaatc tcttcacgtt tcatatcgcg taatttctta ggaataatgc
    20701 ggcaatttga ttgtgcgcaa ttttgtagca tttctccagc actctggaga aataggcaag
    20761 acattggcag aaatgagcat tgagagccag ggcgctggcg atcacaatga aaaacatcag
    20821 gcagatcgtt ctctgccctc atattggccc agcaaaggga gcaagtaatg aacaacttta
    20881 atctgcacac cccaacccgc attctgtttg gtaaaggcgc aatcgctggt ttacgcgaac
    20941 aaattcctca cgatgctcgc gtattgatta cctacggcgg cggcagcgtg aaaaaaatcg
    21001 gcgttctcga tcaagttctg gatgccctga aaggcatgga cgtgctggaa tttggcggta
    21061 ttgagccaaa cccggcttat gaaacgctga tgaacgccgt gaaactggtt cgcgaacaga
    21121 aagtgacttt cctgctggcg gttggcggcg gttctgtact ggacggcacc aaatttatcg
    21181 ccgcagcggc taactatccg gaaaatatcg atccgtggca cattctgcaa acgggcggta
    21241 aagagattaa aagcgccatc ccgatgggct gtgtgctgac gctgccagca accggttcag
    21301 aatccaacgc aggcgcggtg atctcccgta aaaccacagg cgacaagcag gcgttccatt
    21361 ctgcccatgt tcagccggta tttgccgtgc tcgatccggt ttatacctac accctgccgc
    21421 cgcgtcaggt ggctaacgac gtagtggacg cctttgtaca caccgtggaa cagtatgtta
    21481 ccaaaccggt tgatgccaaa attcaggacc gtttcgcaga aggcattttg ctgacgctaa
    21541 tcgaagatgg tccgaaagcc ctgaaagagc cagaaaacta cgatgtgcgc gccaacgtca
    21601 tgtgggcggc gactcaggcg ctgaacggtt tgattggcgc tggcgtaccg caggactggg
    21661 caacgcatat gctgggccac gaactgactg cgatgcacgg tctggatcac gcgcaaacac
    21721 tggctatcgt cctgcctgca ctgtggaatg aaaaacgcga gaccaagcgc gctaagctgc
    21781 tgcaatatgc tgaacgcgtc tggaacatca ctgaaggttc cgacgatgag cgtattgacg
    21841 ccgcgattgc cgcaacccgc aatttctttg agcaattagg cgtgccgacc cacctctccg
    21901 actacggtct ggacggcagc tccatcccgg ctttgctgaa aaaactggaa gagcacggca
    21961 tgacccaact gggcgaaaat catgacatta cgttggatgt cagccgccgt atatacgaag
    22021 ccgcccgcta agctttttac gcctcaaact ttcgttttcg ggcatttcgt ccagacttaa
    22081 gttcacaaca cctcaccgga gcctgctccg gtgagttcat ataaaggagg aacgtatggc
    22141 taatccaacc gttattaagc tacaggatgg caatgtcatg ccccagctgg gactgggcgt
    22201 ctggcaagca agtaatgagg aagtaatcac cgccattcaa aaagcgttag aagtgggtta
    22261 tcgctcgatt gataccgccg cggcctacaa gaacgaagaa ggtgtcggca aagccctgaa
    22321 aaatgcctca gtcaacagag aagaactgtt catcaccact aagctgtgga acgacgacca
    22381 caagcgcccc cgcgaagccc tgctcgacag cctgaaaaaa ctccagcttg attatatcga
    22441 cctctactta atgcactggc ccgttcccgc tatcgaccat tatgtcgaag catggaaagg
    22501 catgatcgaa ttgcaaaaag agggattaat caaaagcatc ggcgtgtgca acttccagat
    22561 ccatcacctg caacgcctga ttgatgaaac tggcgtgacg cctgtgataa accagatcga
    22621 acttcatccg ctgatgcaac aacgccagct acacgcctgg aacgcgacac acaaaatcca
    22681 gaccgaatcc tggagcccat tagcgcaagg agggaaaggc gttttcgatc agaaagtcat
    22741 tcgcgatctg gcagataaat acggcaaagc cccggcgcag attgttatcc gctggcatct
    22801 ggatagcggc ctggtggtga tcccgaaatc ggtcacacct tcacgtattg ccgaaaactt
    22861 tgatgtctgg gatttccgtc tcgacaaaga cgaactcggc gaaattgcaa aactcgatca
    22921 gggcaagcgt ctcggtcccg atcctgacca gttcggcggc taacatgcaa attctcccgg
    22981 tgacggtaat gttccgctac cggacttttc agaaatcatt tattcccctc gcgtcccgcc
    23041 cgttgttact cttccttgtt caggaatgcc aaatataagg acatcatcat gcagagccgg
    23101 aagctcttaa aagaacaact catctatatc cgggataaac gcaacggaga ggtgaaaaac
    23161 agatgaaaat aatacttctg tttttagcag ccctggcaag ttttaccgta cacgcgcagc
    23221 ctccttcaca gaccgtagaa caaacagtcc ggcagattta tcagaactat aaatcagatg
    23281 ccagtacacc ttatttcggt gaaaccggag agcgggcgat aacttcagcg cgtattcaac
    23341 aggcgcttac cctgaacgac aatcttacgc tgccgggcaa tattggctgg ctggattatg
    23401 atccggtttg tgattgtcag gattttggcg atctggtgct agaaagcgtt gcgataaccc
    23461 aaactgacgc cgatcatgcc gatgccgttg tgcgctttcg tatctttaaa gatgataaag
    23521 aaaagaccac gcagacactg aaaatggtgg cggaaaatgg tcgttgggtc attgacgata
    23581 ttgtcagcaa tcatggcagc gtcttacaag cagttaatag cgagaatgaa aaaacgctgg
    23641 ccgctttagc ttcgttgcaa aaagaacagc cggaagcctt tgttgccgaa ctctttgaac
    23701 atattgctga ttatagctgg ccgtggacgt gggtggtttc cgactcttac cgccaggcgg
    23761 ttaatgcctt ctataaaacc accttcaaga cggccaataa tcccgatgaa gatatgcaaa
    23821 tagaacggca atttatttac gacaatccga tctgttttgg cgaagagtcg ctattttcac
    23881 gcgttgatga aattcgagtc ctggagaaaa ccgccgattc cgcccgcatt catgttcgtt
    23941 ttacgctgac caatggcaac aacgaagagc aagaactggt tttacagcgg cgcgaaggca
    24001 agtgggaaat cgctgatttt atccgcccga acagcggcag cctacttaag cagattgagg
    24061 caaaaaccgc cgccagatta aagcaatgag ctgaattaaa taacaattag ccggaacaat
    24121 aaataaaagg gaacactata tgaaaacgat tttcaccgtg ggagctgttg ttctggcaac
    24181 ctgcttgctc agtggctgcg tcaatgagca aaaggtcaat caactggcga gcaatgtaca
    24241 aacattaaat gccaaaatcg cccggcttga gcaggatata aaagcactac gcccacaaat
    24301 ctatgctgcc aaatccgaag ctaacagagc caatacgcgt cttgatgctc aggactattt
    24361 tgattgcctg cgctgcttgc gtatgtacgc agaatgataa aaaaatcccc ggcagcatgt
    24421 cagttgccgg ggattttttt taacgtccaa ccgccgcttt agggcgtttc ttcgcaccag
    24481 cattcaccgg acgagattgc gtagacgacg ctttttttgc cgtagcaggc gtctgacgct
    24541 gggtcgccat cggcgtatgt ttagtcaacg ccggacgggt attgcggttc tggcgacgag
    24601 cttcacgcat ctcttcaatg gttggcgcag gcactaagca atcgcgacgg ctgccaatca
    24661 gatgcttttt gcccatcgct tccagcgccg ggcggattaa cggccagttt gccggatcgt
    24721 ggtaacgcaa caacgcttta tgcaaacgac gctgtttgtc gcccttcggt acgaagacgt
    24781 cttcgctctt ataaccaatc ttcgccagcg gttttttccc ggtgtaatac atggtggttg
    24841 agttcgccag cggcgatggg tagaagttct gtacctggtc gagacggaaa cgatgctttt
    24901 tcagccacag cgccaaattc accatatctt catcacgcgt accggggtgc gcagagatga
    24961 aatacgggat cagatactgc tctttacctg cctgtttcga gtaggtatcg aacagctctt
    25021 taaagcggtc atagctgccc atgcccggct tcatcatctt cgacaacggc ccttcttcgg
    25081 tatgttccgg agcaatcttc agataaccgc cgacgtgatg ggtcgccaac tctttgatat
    25141 agcgcggatc ttccacggcg atgtcataac gtacgcctga ggcgatgagg atttttttaa
    25201 tgcctttcag atcacgcgca cggcgataga ggttgatcgt cggttcgtgg ttagtgtcca
    25261 tgtgcggaca aatatccgga taaacgcacg acaaacggcg acaagtttgt tcagcgcgtg
    25321 gcgatttgca gtgcaacata tacatgttgg cagttggccc accgagatcg gaaatcacgc
    25381 ccgtaaaacc tggaacggtg tcgcggatcg cttcgatctc attaatgatc gaatcttccg
    25441 aacggctctg aataatgcgc ccttcgtgct cggtaataga acagaaagaa cagccgccaa
    25501 agcagccgcg cataatgttg accgaaaaac ggatcatttc gtaagccgga atacgggcat
    25561 tgccataggc cggatgtggc acgcgcttgt acggcagcgc aaaaacgctg tccatctctt
    25621 cggtagaaag cgggatagca ggcgggttga tccacacata gcggtcaccg tgtttttgca
    25681 tcaatgcgcg gacgcagcct gggttggttt cgtggtgcag aatacgcgaa gcatgggcgt
    25741 acagcacttt atcgcccttc actttctcga aagaaggcag caacacgtag gttttttccc
    25801 acggtttcgg gcgcggtggc tgcacggtta cggctttggc ttcctgcttt ttcggtgcca
    25861 ccggtttgtt atccgcgcac ggcaaatctt caccatacgg atgcgggatt gggtcgattt
    25921 ttccaggggt atcaagacgg ggggaatcca cgccgctcca gccaggcagc gcctctttca
    25981 cgataatcgc ggtattacgc acatcgcgga tttcactaat cggctcgccc atcgccagac
    26041 ggtgcgccac ctccaccagc ggacgctcac cgttaccaaa catcagcatg tcggctttcg
    26101 aatccaccag cacggaacgg cgcacggtat cggaccagta atcataatgt gcggtacggc
    26161 gcagactagc ctcaataccg ccgaggatca ccggtacatc tttccacgcc tctttacaac
    26221 gctgggtata aaccagtgtg gcgcgatccg ggcgcttacc cgcgacgtta tccggcgtgt
    26281 aggcatcgtc atgacgtaaa cggcgatcgg cggtataacg gttgatcatc gaatccatgt
    26341 tgccagcagt aacaccgaaa aacagattcg gtttacccag acgcataaag tcgtctttgc
    26401 tgctccagtc cggctgggcg atgatcccga cgcgaaagcc ctgtgcttcc agcatacgac
    26461 cgcaaatcgc catcccgaag cttgggtgat cgacatacgc gtcgccagta accaaaatga
    26521 tgtcgcagct atcccagcca agttgatcca tctcttcacg agacatcggc aaaaacggtg
    26581 ccggtccaaa acaggcggcc cagtactgcg gccaggagaa caggtcgcga tccggttgga
    26641 tcagggagat agagctcatt ttgcttccag aaatgataaa aaaataatca aaggccgggg
    26701 attataagcc ggaacgaaag agaaatcgaa aagtattcca tactcgccct cctcgggcga
    26761 gtatgaagat tacggtaccg gattgactaa cagttgccca atcgagccac ggtccgccat
    26821 ttccagcgtc tgactgttga agtagaacgg gaagtgcgcc caggaaggct gaccgaaata
    26881 aacaagcagc tccacttgtc cgtcaaccca aacggtatct ttccagcctc tgtcttccgg
    26941 gaacggcatc gcaccattca cgttacggat ctggaacatc acgccttcaa tatggaacgc
    27001 ttgcggctcg tccgcgcgta ccgtccagcg ttcccacgtt ccttgctgcg cggtgacatc
    27061 aatacggttg acgtcccaca gctgtccatt aatacccggg tcatcaccca gactgatatc
    27121 gcgactgcga atcggcgaac cggccatgat ttcagttggc agcaagcgca tcggaagact
    27181 gtctgtgacc agcggcagaa ggccggttgg gcgcagcgtt agcaccaggg tagaaaccag
    27241 aatactggat ggctcaaaga agccgcgaat acgatcaaca atgctcgccg cttcgccaca
    27301 ggtgatcgac acttcatcgc cgttgctcat atccaccaga atctcgcggc gctcgcccgg
    27361 tgccagcgaa agttgcttca ccgatacagg agcaggcagg aatccctgat cgccagaaat
    27421 cacatgtaac gggcgaccat cgctcatctg tagttgatag cgacgagagt tcgacgcgtt
    27481 cagcagtcgc aagcgcaccc agccacgcga gacttcaacg tacgggcttt gtacaccgtt
    27541 aaccagcagc gtatcaccaa caaagccgcc gcttcccggt tcgttgtatt ctggcgtacc
    27601 aaagttatcc agccgtttat cctggataat gaccggaaaa tcatccacat cataatggtt
    27661 ggggataggc agcgacttgc tgacttcatc ttccaccagc cacattccgg caaggccgtt
    27721 atagacctgc tgagccgtgc ggttgggagt attggcgtga taccacagag tagctgcgtt
    27781 ctggcgaata ggcagtacgg gtgcccagtc agcgtttggc gacatcatcc gtgccggacc
    27841 gcccatcagc gggccaggta cctgtagccc ggccaccgtc attgagacat tttctgtcag
    27901 gcggttgctg taaataagct taacatcgtc gcccttccag acgcggatag tcggccccag
    27961 gtaacgacca ttgattcccc agaccgacgc gcgtgtccct ggcgtaaatg accagtgcgc
    28021 acgttgtaca gtcataaaca gcggttgccc acggcgagat tcaagtagcg gcggaacggg
    28081 tagcggttgc tgttgcccgg ctgcgctggc cttcagggga acagcgcctg cacaaagtgc
    28141 aatccccgat gcctgaatga actgacgccg actgagtgac atatttgctc catgtaaaac
    28201 tggctaaagt aataccgtgc aggaaagtgt tccccgcaaa aatacagatt gccgacttaa
    28261 acttttccgg cggcttcgcg ttctgcgact tctttatcga gctcggcgat tttttgttcc
    28321 attatcgaac gacaatgggc agccagctca cgaacctgat ctttgccata ctgactgacg
    28381 tcaattggcg gcagcatttc gacaatcacc agaccgttgt gcagtcgatt aagattaatc
    28441 ttattcgaag ttgtagagac gcacacggga ataatcggga cgcccgccgc aattgccgcg
    28501 tgaaatgctc cagtcttgaa cggtagcagg ccgcgaccac ggctgcgggt tccttccggg
    28561 aacatccaga tggaaatacg gcgttttttg aaatgattca cgacttccgc aatggtgccg
    28621 tgagctttag tgcgattgtt tctgtcgatc aataagttgc cggttaacca gtacaactgc
    28681 ccgaagaagg ggatccacag caagctcttt ttacctaccg tcaccgtcgg cggttgcaca
    28741 atgttcgatg ctgtcaccat gtcatagttg ttctggtggt tagcgatata gatagcattg
    28801 ccgtagcttt cagcatctgc aggtttgcgg cactcaactt tcaggccgaa cagcggcgca
    28861 agacggccaa acatatgccc aaaggtggcc acatgtttcg ggttacgcgg gctgaaaagg
    28921 cagtaaatgg agccgaatac acagactaag atgctgtaaa tcacggtaat aataagacga
    28981 aagatatata gcataacacc ctctgaaggt ctgagagact ggcattgtac gtcacctgaa
    29041 atcaggttag ggcattgtta aaagccgctc tctggaaaag cgtattgtta atcgcaacgc
    29101 atgaataaac aacggtttca agggaatttt tatcgtttcc gggggttgaa tacgctgccg
    29161 gatgacgact taacgtttca tccggcgttc cttgcaagcg ggaggaaaca ggccctcccc
    29221 ggcatattac tcttcgctat caccgttgct ggcacggcga ggagagtcga tctcaacacg
    29281 atcgatacgc tgcaaaccgc gcatcaacgt accgcggcgt ccacgttcac cagtgacttt
    29341 ctgtaactct tccgggcgca gtttaatttt gcgtttccca acatgaatgg tcagcgtgct
    29401 ttgcggcggc agaacgtaca attgcgccag accatcttct ccacgcgcgg cttctgccga
    29461 tggaatgttg ataatcttgt tgcctttgcc cttcgacagc tgcggcagat cgcttaccgg
    29521 gaacatcaac atacggcctg cctgagtgat tgccagcagc atatcggaag catcttcaat
    29581 caccaccggc ggcataacat gggcattttc cggtaaggtg atcaaagcct tacctgcacg
    29641 gttacgcgcc accagatcgt taaaggtgca gacgaaaccg taacccgcat cggaagccat
    29701 cagcagtttc tgatcgtcgc tttccatcag catatggtca acggtcgccc caggcggcaa
    29761 cgttaatttg ccggtgagtg gttcgccctg accacgcgcc gacggcagcg taatcgggtc
    29821 gatggcatag ctacgaccgg tggaatcaac aaacactacc gattggttgc tcttaccttt
    29881 caccgccact ttgaagctat cacccgcttt ataattcagg cccggcgcgt cgatatcatg
    29941 gcctttagcg ctgcgtaccc agcccatctg cgacagcaca atggtgacag gttcagacgg
    30001 cagcatgtcg tgctcgctca tcgctttcgc ttcttcgcgt tcctgcaacg gcgaacgacg
    30061 atcgtcaccg taggcttgcg cgtctgcctg cagttctttc ttcagcaggt tattcatttt
    30121 acgctcggaa gccaaaatgc cctgcaactg gtcgcgctct ttttccagct cactctgctc
    30181 accgcgaatc ttcatctctt ccagtttggc aagatgacgc agtttcagtt cgaggatcgc
    30241 ttccgcctgg gtttccgtaa ggccaaaccg cgacatcagc gccggtttcg gttcatcttc
    30301 gttacgaatg atctcaatca cttcgtcgat gttgagaaac gccaccagca aaccttccag
    30361 gatatgcagg cgcttgagga ctttctccag acgatagttc agtcggcggc gcacggtatc
    30421 gcgacggaac accagccatt cggagaggat ttccagcagg tttttcaccg ccggacgacc
    30481 atccagaccg atcatattga ggttaatgcg atagctcttt tccagatcgg tggtagcgaa
    30541 gaggtggttc atcacctgat ccatatccac gcggttggaa cgcggcacaa tcaccagacg
    30601 ggtcgggttc tcgtggtcag attcatcgcg cagatcgtca accatcggca gctttttgtt
    30661 gcgcatttgc gcagcaattt gctccagtac acgcgcacct gaaacctgat gcggtaatgc
    30721 gctgataacc accgcgccat cttctttctt ccacaccgcg cgcatacgta ctgaaccacg
    30781 tccgttctcg tagattttac ggatctcggc gcgcgaagtg ataatttccg cttcagtcgg
    30841 ataatccggc ccctgcacga tatccagcag ctgatcgagc gtggttttcg gctggtcgat
    30901 taatgcgatt gccgcctgag ctacttcacg caggttatgc ggtggaatat cggtcgccat
    30961 gccgacggca ataccggtgg tgccgttaag caaaatgttt ggcagacgag caggcagcat
    31021 tttcggctcc tgcaaagtgc cgtcgaagtt tggcacccag tcagccgtcc cctgccccag
    31081 ctcgctcaat agcagctcgg aatatttcga caaccgggat tcggtgtaac gcattgccgc
    31141 gaacgatttc ggatcgtccg gcgcgcccca gttcccctga ccatcaacca gcggataacg
    31201 gtaagagaac ggctgcgcca tcaggaccat cgcttcataa caggcgatat cgccgtgcgg
    31261 atggtattta cccagtacgt caccgacggt acgggccgat tttttaaatt tggcgctggc
    31321 attcaggccc agttcagaca tcgcatacac aatgcggcgc tgaacaggtt tcagaccatc
    31381 accaataaac ggcaacgcac ggtccatgat cacgtacatg gagtagttta agtaggcgtt
    31441 ttccgtaaat tcatgtagcg caaggcgctc tgccatatcg ctcattaatt ctgattcctc
    31501 aactgattcg ccagccttca aaggggcaat attgccggag atactacatt atctgccgcg
    31561 tcgagtcaca ggagatcagc gtgaatagcg ccgccggacg tgctccgacg gcgataaagg
    31621 catcaatgtg ccttgatata caacgctttg ctgtagactt gatcggcata gagggcagca
    31681 caaggttgcg cccatgccac accactcggc atgggggtcc gctccgggcg attcgagaag
    31741 ttgaggttat gcgagtccag ggaagcctaa agcatcaaag tagtcgactg taagtatatg
    31801 gttcagccca gtttagctgt gtctccacca gtgccccgcg cctcctgcca taagtgcggc
    31861 ttgctcatgg gtacccccga gacggcttac ggcgcaccgg ataataaatt tcgcgtatac
    31921 atggtattcc ttaccggaga aattataagc ccaacaacaa tacgcctgtg gcattgttcg
    31981 ctcaaactta tcgcgagtga tttggctcac actccggtaa agattcccca tcacgctaac
    32041 ggcacataaa catccgtttc cagtcgctgc tccgtcaccc cttcggcaag attggtgtag
    32101 tgaaacagaa tcggtgcttt acgcattttc tcgccgcttg caggcagcca gtggcgaata
    32161 atgccccata tcgtgtgtga aatatcgtcc agctcgccaa cgtggcgggc cacggcataa
    32221 cgtccaccgg taagttcacc attgctcaca ccataacgat tatcgggaat cggttcgcta
    32281 acgctgccgc agatatcaaa gcgaaacgct tccggtgcgg tggtggcagg gtcgtcccag
    32341 gcgacgccaa aagtctggct ttggttaaca ggagaaagcc ccgtctcttt acgccacatg
    32401 ataaatttcg ccacactgta gttgagcaac tcagggctac agcggtgcgg caacatcgcc
    32461 acagggattg atgggaaatc gataatgttt acatccagtg tcaggtttgt cattttgcct
    32521 ccctgcgaaa ttattcccgg gaacgcgtcc cgggagcggt aacaattacg gatttacttt
    32581 gcggatctgt ttgacgtcaa tttcaacaga attccagtct ttatcgactt caccctgaat
    32641 ctcaaccgta tctttcggcg tcaccgtcac accgttccag cgtttgtggt cgatatcaac
    32701 attgatagta ccgctggcat ctttaaacac gtagagatcg tcagagatgc gttcaacgat
    32761 attgccgcgc agggttaccc aggtgtcgtc acgcagggat tttgcgcttt ctacagtcgt
    32821 tacgctgccg ttcggcccct ggaatcctcc ggcctgactt tgcgttgccg atgggccaga
    32881 aaaaccgccc tgctctgctg ccatcaccgg tgcactgcac agggccatta ctgcgattac
    32941 tgctgcgaat tttttcatgt ttattactcc ctttaatgtc tgtttccgag catttaacaa
    33001 gatagtcctt aacaacttct taagggaaaa aaataaaatt taatgctgta cagagcgcgt
    33061 tacaacacgg tttactggca gcaaatacgg ttatcgcagg gatgaaaaaa tgcgaatttt
    33121 actgatagaa gatgacatgc tgattggcga cggcatcaaa actggcctta gtaaaatggg
    33181 ttttagtgtc gactggttta cacaaggtcg tcagggaaaa gaggcgcttt atagcgcacc
    33241 ttatgatgcg gtgatcctgg atttaacctt accaggcatg gatggtcgcg atattttgcg
    33301 cgaatggcga gagaaaggtc agcgtgagcc ggtactgatc ctgaccgcgc gtgatgcgtt
    33361 agcggaacgt gtagaagggc tgcgtctggg agctgacgat tatctgtgta aaccttttgc
    33421 gttgatagaa gtcgccgcca ggctggaggc tctgatgcgc cgaaccaacg gccaggccag
    33481 caacgagctg cgccacggta acgtcatgct cgaccccggc aaacgtatcg ccacgctggc
    33541 tggcgaaccc ttaacactga aaccaaaaga atttgccctg ctggaattac tgatgcgtaa
    33601 cgctggtcgg gtactgccgc gcaaactgat tgaagagaaa ctgtatacct gggacgaaga
    33661 ggtcaccagt aatgccgttg aagtgcatgt gcatcatctg cgacgcaaac tcggtagtga
    33721 ttttattcgt accgtgcatg gtattggcta cacattaggt gagaaatgaa atttacccaa
    33781 cgtcttagtc tgcgcgtcag gctgacgcta atctttttaa ttctggcctc ggtgacctgg
    33841 ctgctttcca gctttgtcgc ctggaaacaa acaacggata acgtcgatga attgttcgac
    33901 acccaactga tgctgtttgc caagcggtta agtacgctcg atctcaacga aatcaacgcg
    33961 gcggatcgca tggcacagac gccaaataaa ttaaaacacg gtcatgttga tgacgatgcg
    34021 ctgacctttg ccatctttac ccacgacggc agaatggtcc ttaatgatgg cgataacgga
    34081 gaagatattc cctatagcta tcaacgggaa ggttttgctg acgggcaact ggtcggtgaa
    34141 gacgatcctt ggcgttttgt ctggatgacc tcacctgatg gcaaatatcg catcgttgtt
    34201 ggccaggaat gggaataccg tgaagacatg gcgctggcga ttgttgccgg gcaattgatc
    34261 ccgtggctgg tcgcactgcc gattatgtta atcatcatga tggtactact gggtcgtgaa
    34321 ctcgcgccgc tgaacaaact ggcgctggca ctacgtatgc gtgaccctga ctcggaaaaa
    34381 ccactaaacg cgactggcgt acccagcgaa gtgcgaccac tggttgagtc gctaaatcaa
    34441 ctgttcgccc gcacacatgc gatgatggtt cgagaacgac gctttacctc cgatgcagct
    34501 cacgaactac gtagcccgtt aacagcgctg aaagtgcaaa ccgaagttgc gcagctctct
    34561 gacgatgatc cgcaggcgcg gaaaaaagca ctgctccaat tacattccgg gatcgatcgc
    34621 gctactcgtc tggttgatca actgctcacg ctatcgcggc tggactcact ggataacctt
    34681 caggacgtcg cggagatccc gcttgaagat ctcctgcaat cgtcggtgat ggatatttac
    34741 cacacggcgc agcaggcgaa aattgacgtg cgactgacac tcaatgccca cagcatcaaa
    34801 cgcactggac aaccgctatt gctaagtttg ttggtgcgaa accttctgga taacgcagtg
    34861 cgctacagtc cacagggcag cgtggtggac gtcacgctga atgccgataa tttcatcgtg
    34921 agggataacg gccccggtgt gacaccagag gcactggcgc gaattggcga acgcttctat
    34981 cgcccccccg gacaaaccgc taccggcagt ggacttgggc tatcgattgt ccagcgaatc
    35041 gccaaattgc atggcatgaa tgttgaattt gggaatgcgg aacaaggtgg atttgaggcg
    35101 aaggtgagct ggtaagggta agacttttgc taaattcgca aaagactttg cacattttgc
    35161 taattttacc gtaccgctct gtgacgtact atagtcgaca aacgtctcac cttgaggtta
    35221 aaaaatgagc aacaccctga ttatcaacgg cgcgaaaaaa ttcgcccact ccaatggtca
    35281 actgaacgac accctgaccg aagtcgcgga tggcacactg cgcgacctcg ggcatgatgt
    35341 tcgcatcgtt cgcgccgaca gcgactacga tgtcaaagcg gaagtacaaa actttctctg
    35401 ggctgatgtg gtgatctggc agatgccagg ctggtggatg ggcgcgccgt ggacagtgaa
    35461 aaaatacatt gatgatgtat tcaccgaagg tcacgggacg ctgtatgcca gcgatggccg
    35521 tacccgcaaa gatccgtcaa aaaaatacgg ttccggcggc ctggtacagg gcaaaaaata
    35581 tatgctttct ctgacctgga acgcaccaat ggaagccttc accgaaaaag atcaattctt
    35641 ccacggcgtt ggcgtcgacg gtgtgtatct gccgttccat aaagcaaacc aattccttgg
    35701 catggaaccg ctgccgacat ttatcgctaa tgacgtgata aaaatgcctg atgttccccg
    35761 ctatactgaa gaatatcgca agcatcttgt ggaaattttt ggttaactag agctcaggct
    35821 ttagaaggag ttaaccatgc ttaccgtaat cgcagaaatc cgtactcgtc ctggtcaaca
    35881 tcaccgtcag gcggtattgg atcagtttgc taaaatcgtt ccaaccgtac tgaaagaaga
    35941 aggttgccac ggctatgcgc caatggtgga ttgcgctgct ggcgtgagtt tccagtctat
    36001 ggcaccggat tctatcgtga tgattgagca gtgggaaagc atcgcgcacc ttgaagcgca
    36061 tctgcaaacc ccgcacatga aggcgtacag cgaagccgta aaaggcgacg tgctggagat
    36121 gaatattcgt attctgcagc cagggattta atcctgcctt gtttgcccgg ccatcctgac
    36181 cgggcaatgt tctttccttt aaacctcaat ctccgccatg tcgcctttct cttgcaacca
    36241 gttgcggcga tcttccgagc gtttcttcgc cagcagcata tccatcatcg cgtcagtacg
    36301 ctgatcgtct tcatcatcga tagtcaactg caccagacgg cgagtgttcg gatcaagcgt
    36361 ggtttcacgc aattgcatcg ggttcatttc ccccagacct ttaaaacgct ggacgttcgg
    36421 cttgcctttc ttgcgtttta attgctcaag tacgccctct ttctcttctt ccgtcagcgc
    36481 gtaataaacc tctttcccga gatcaatacg gtagagcggt ggcagtgcga cgtaaacgtg
    36541 accgtgtttc accaacgcgc ggaagtgttt tacgaacaaa gcgcagagca gcgtggcaat
    36601 gtgcagacca tcagagtccg catccgcgag gatacagatt ttgccataac gaagctggct
    36661 cagatcgtcg ctgttaggat cgataccgat cgctaccgaa atatcatgca cttcctgcga
    36721 ggccagcact tcgtcggaag aaacttccca ggtgttaagg atcttacctt tcagcggcat
    36781 gatcgcctga tattcacgat cgcgcgcctg cttggcagat ccgcctgcgg agtcaccttc
    36841 cacgaggaac agttcggtac ggttaaggtc ctgcgcggta caatcagcca gtttgccagg
    36901 cagcgccggg ccgctggtca gctttttacg caccactttt ttagccgcac gcatacgacg
    36961 ctgggcgctg gaaatcgcca tctctgccag caactccgcc gcctgaacgt tctggttcag
    37021 ccacaggata aaggcatctt tcaccacgcc agaaacgaat gccgcgcatt gacgcgaaga
    37081 gagacgctct ttcgtctgcc cggcaaactg cggatcctgc attttcaccg acagaacata
    37141 ggcgcagcga tcccagatat cttccgccga cagcttcacg ccgcgcggca gaatgttgcg
    37201 atattcacag aactcacgca tcgcgtccaa caggccctga cgcaggccat taacatgggt
    37261 accgccctgc atcgttggga tcaggttgac gtagctttcg gtcagcagtt caccgccttc
    37321 cggcagccac agtagcgccc agtccacagc ttcagtatcg ccagcgaaat taccgataaa
    37381 cggtttttct ggcagcgtcg gtaaaccgtt taccgcttcc gccaggtaat cattcagacc
    37441 gtcctgatag caccagcgct gttcggtgtt gttgatctca tctttaaagg taatctcaac
    37501 gccggggcac agtaccgctt tggctttcag cacatgcgtc aggcgtgaaa cagaaaatcg
    37561 cgggctgtca aagaaggttt catccggcca gaagtgcacg ctggtaccgg tattgcgttt
    37621 accgcaagtg ccgacaacct gtaaatcctg caccttttcg ccattttcaa aggcgatgtt
    37681 ataaacctga ccatcgcggc gcacgttaac ttcaacgcgc ttcgacaggg cgttaaccac
    37741 cgaaatcccc acgccatgca ggccgccaga gaactggtaa tttttgttag agaatttacc
    37801 gcctgcatgc agacggcaaa gaatcaattc aaccgccggg acaccctctt ccgggtgaat
    37861 atccaccggc atcccgcgcc catcgtcaat aacttctaac gactggtcag catgtaagat
    37921 tacatccacg cgttttgcgt gacccgccag tgcttcatcg acactgttat caataacttc
    37981 ttgccccaaa tggttagggc gagtggtatc ggtatacatc cccggacggc ggcgaaccgg
    38041 ctcaagcccg gtgagtacct caatggcatc agcgttataa gtttgcgtca tggtttaagt
    38101 tagcaattcg agttgatcgt cagagatggt gcagaccaag aaaatcgacg atcgggttga
    38161 aataatcttc gaagcccgtg aatgcgtggt tgccgccttc tatgacagtc tggcggcagg
    38221 aagcgtagta cgccaccgcc tggcggtaat ccagcacttc atctcccgtc tgttgcagca
    38281 gccagatcaa atccggcgct tccagcgggt caatctgcat gactttgaga tcgtaaatat
    38341 ggcgtgactc tagcacatat tgctgcccgg tgtatgggtt ctcgttctga ccgagatagt
    38401 ccgtcagcag ttcaaacggg cgcaccgccg ggtttaccac cactgcgggc agcataaaac
    38461 attgtgacaa ccaggtggcg taatatcccc ccagtgacga accgacaata cccagcgaat
    38521 cgccgccatg ttccaggaca atggattcca gcagttctgc cgcgtcggaa ggatacggcg
    38581 gcaactgcgg aatgatcatc tcaacgtcag ggtgatgttc cgccagccag ttttttaaca
    38641 agctcgcttt tgcagagcgc ggcgagctgt tgaaaccgtg taaataaaga agcgtagaca
    38701 tcagtagcct tctgaagcgg tatcaggttg gaaacgtgtg tccgccaggc gatgcacctc
    38761 ggtggtcagc gtgccatcag catgtaactc gagagtacgc cagccgggcg cgatagtatc
    38821 cagcgtaaag ttggaacagt gcggcttaaa ctgcacacag gtcgacggcg ttgccagcag
    38881 gcggcgaccg ttccagtcga gatccagctc ctgatgaata tgaccgcaca gcaagtattt
    38941 gacgtgcgga aacttcgcca gcacggtatc cagttcgccc gcgttacgca gactgtgttg
    39001 atcgagccaa ctacaacccg caggtagcgg atgatgatgc agcagcagca acgtatggcg
    39061 ttctggcgca tcggccagtt tacgttccag ccactcaagc tgaaactcgc tcagctcacc
    39121 gtgcggcacg ccaaacacct ggctatccag caacaggatt tgccattgct caccaataaa
    39181 cacccgcttc gccggggaga tacccgcatc ctgtaacgcg ctgtacatcg cgggctggaa
    39241 atcgtggttg cccggcagcc agacgcaggg cgcacgaaaa cttgcgatgc cttcagcgaa
    39301 atgctgatag gccgcagagg attgatcctg cgctaaatca cctgtcgcga caatcaggtc
    39361 gaattcgtgc tgatgtggac gaatcgcctc cagcaccgcc tggtagcttt cccaggtgtt
    39421 tacccctaac agagcttcgt gcttttgtgc aaacaggtga gtgtcggtaa tttgtaaaat
    39481 cctgactctg gcctcaccag ccagaggaag ggttaacagg ctttccaaat ggtgtcctta
    39541 ggtttcacga tgctagtaaa ccggaatcgc catcgctcca tgtgctaaac agtatcgcaa
    39601 ccagtccgct aaaaactgat taatttgatg cttttcgtcg cgttgatgca actttttatt
    39661 aggataatca taccgcgctt tgaagcgaaa aatctgctgg cttgaacaca cttcagccac
    39721 catcgcgtca tgatacagac gcaccgtcat tgacggaagg ctccagtaac tgatcgcggg
    39781 cgcagtctgt tctattgtca ccagggtagt gtatcgggtc gattccacaa tcgtcagccg
    39841 atattgtgcg tttgccacct gatagcttac agtttcgccg ggtgcgtcat tgcgcggtaa
    39901 caaacggcgc aattgtgaaa agttcatctc gcacaggcgc atcatttcag gaaagtcagg
    39961 tgtgtaacgc ttcatttatg cccactcatt ttttaacgct tgatgatgca gctgcagcca
    40021 ttgcaaagcg atgaccgacg ctgcgttgtc gattttcccc tcttctaccc actggtatgc
    40081 ctgttcccgg cttaccacat gaacgcgaat atcttcgttt tcatcagcca gaccatgaat
    40141 accgcttgcg gtcgtggcgt ccacttcgcc caccataatt gacgaacgct cactggtgcc
    40201 ccccgggctt gccaggaaac ttaacaccgg tttggtccgt ttgactatca gtcccgcctc
    40261 ttcaatcgct tcgcgacggg caacatcttc cacactttca ccctcttcta tcatcccggc
    40321 aaccatctcc agtagccagg gggtttcact ggtgtcgtat gcggcaatcc gaatctgctc
    40381 aatcagcaca acttcatcgc gcactgggtc aaagggtagc aagactgcgg cgtgaccgcg
    40441 ctcaaaaatt tcccgccgta cctcatgact catttgcccg ttgaatagac gatgacgaaa
    40501 tctataaaga tctaatgaaa aaaagccgcg ataaagtgtt tctcgtgcaa taatttctac
    40561 atcgtttttg ccaaatgtaa cgggcaggtt gtctggctta agcattgtta atgtcctggc
    40621 actaatagtg aattaaatgt gaatttcagc gacgtttgac tgccgtttga gcagtcatgt
    40681 gttaaattga ggcacattaa cgccctatgg cacgtaacgc caaccttttg cggtagcggc
    40741 ttctgctaga atccgcaata attttacagt ttgatcgcgc taaatactgc ttcaccacaa
    40801 ggaatgcaaa tgaagaaatt gctccccatt cttatcggcc tgagcctttc tgggttcagt
    40861 tcgttgagcc aggccgagaa cctgatgcaa gtttatcagc aagcacgcct tagtaacccg
    40921 gaattgcgta agtctgccgc cgatcgtgat gctgcctttg aaaaaattaa tgaagcgcgc
    40981 agtccattac tgccacagct aggtttaggt gcagattaca cctatagcaa cggctaccgc
    41041 gacgcgaacg gcatcaactc taacgcgacc agtgcgtccc tgcagttaac tcaatccatt
    41101 tttgatatgt cgaaatggcg tgcgttaacg ctgcaggaaa aagcagcagg gattcaggac
    41161 gtcacatatc agaccgatca gcaaaccttg atcctcaaca ccgcgaccgc ttatttcaac
    41221 gtgttgaatg ctattgacgt tctttcctat acacaggcac aaaaagaagc gatctaccgt
    41281 caattagatc aaaccaccca acgttttaac gtgggcctgg tagcgatcac cgacgtgcag
    41341 aacgcccgcg cgcagtacga taccgtgctg gcgaacgaag tgaccgcacg taataacctt
    41401 gataacgcgg tagagcagct gcgccagatc accggtaact actatccgga actggcggcg
    41461 ctgaatgtcg aaaactttaa aaccgacaaa ccacagccgg ttaacgcgct gctgaaagaa
    41521 gccgaaaaac gcaacctgtc gctgttacag gcacgcttga gccaggacct ggcgcgcgag
    41581 caaattcgcc aggcgcagga tggtcactta ccgacgctgg atttaacggc ttctaccggg
    41641 atttctgaca cctcttatag cggttcgaaa acccgtggtg ccgctggtac ccagtatgac
    41701 gacagcaata tgggccagaa caaagtgggc ctgagcttct cgctgccgat ttatcagggc
    41761 ggaatggtta actcgcaggt gaaacaggcc cagtacaact ttgttggtgc cagcgagcaa
    41821 ctggaaagcg cgcatcgtag cgtcgtgcaa accgtacgtt cctccttcaa caacattaat
    41881 gcatctatca gtagcattaa cgcctacaaa caagccgtag tttccgctca aagctcatta
    41941 gacgcgatgg aagcgggcta ctcggtcggt acgcgtacca ttgttgatgt gttggatgca
    42001 accaccacgc tgtacaacgc taagcaagag ctggcaaatg cgcgttataa ctacctgatt
    42061 aaccagttga atattaagtc agctctgggt acgttgaacg agcaggatct gctggcactg
    42121 aacaatgcgc tgagcaaacc ggtttccact aatccggaaa acgttgcacc gcaaacgccg
    42181 gaacagaatg ctattgctga tggttatgcg cctgatagcc cggcaccagt cgttcagcaa
    42241 acatccgcac gcactaccac cagtaacggt cataaccctt tccgtaactg acgacgacga
    42301 cggggcttcg gccccgtctg aacgtaaggc aacgtaaaga tacgggttat ctgccgcatt
    42361 cttccccctt ctcgcttcaa tttcgaccag ccatcctcta ttctgatggg tatttaccac
    42421 tggtcccgga agacaaaaat gaaacggaca aaatccatac gccacgcatc gttccgcaaa
    42481 aactggagcg cacgccatct gacaccagtc gctctcgcgg ttgccactgt ttttatgctg
    42541 gctggctgtg aaaagagtga tgaaacagtg tctctctatc aaaatgctga cgactgttca
    42601 gctgcaaacc caggcaaaag cgccgaatgt accaccgcgt acaacaatgc gctgaaagaa
    42661 gccgaacgta ctgcgccgaa atacgccacc cgtgaagact gtgttgctga atttggtgaa
    42721 ggtcagtgcc agcaggcacc agcccaggct ggcatggcac cagaaaacca ggcgcaggcc
    42781 cagcaatcca gcgggagttt ctggatgccg ctgatggccg gttacatgat ggggcgtctg
    42841 atgggcggcg gcgcgggatt tgcacagcag ccgctgttct cctcgaaaaa cccagccagc
    42901 ccggcttacg gtaaatatac cgacgcgacg ggtaaaaact atggcgcagc ccagccaggc
    42961 cgcaccatga ccgtaccgaa gacagccatg gcaccaaaac cggcgaccac tactaccgtt
    43021 acccgtggcg gttttggtga atctgttgcc aaacaaagca ctatgcagcg tagcgcaacc
    43081 ggcacctctt ctcgttcaat gggtggctga taccgatgga aagagtcagt attaccgagc
    43141 gcccggactg gcgcgaaaaa gcccacgaat acggtttcaa ttttcacacc atgtacggcg
    43201 agccgtactg gtgtgaagat gcttactaca agttgaccct cgcccaggtt gaaaagctgg
    43261 aagaagtcac cgccgaactg caccagatgt gcctgaaagt ggtggaaaaa gtgatcgcca
    43321 gcgatgagct gatgaccaaa ttccgcattc caaaacgcac ctggagtttt gtgcgtcagt
    43381 catggctgac tcaccagcca tcgctttatt cgcgtcttga tctggcatgg gatggcactg
    43441 gcgaacctaa actgctggaa aataacgccg atacaccaac gtcactatac gaggcggcgt
    43501 tctttcagtg gatctggctg gaagatcagc ttaacgccgg taacttgccg gagggcagcg
    43561 accagtttaa cagtctgcaa gaaaagctaa tcgatcgctt tgtcgagttg cgtgaacagt
    43621 atggcttcca gttgctgcat ctcacctgct gtcgcgacac ggtggaagat cgcggaacca
    43681 ttcagtattt gcaggactgc gcgacggaag ctgaaatcgc taccgagttc ctctacattg
    43741 acgatatcgg gttaggtgaa aaaggtcagt tcacggattt acaggatcag gtaatttcca
    43801 acctgttcaa gctgtatcca tgggaattta tgttgcgtga gatgttctcc accaagctgg
    43861 aggatgcagg cgtacgctgg ctggaaccgg cgtggaagag cattatctcc aacaaagcgc
    43921 ttctaccgct actatgggag atgttcccga atcacccgaa cctgctgccc gcttattttg
    43981 cggaagatga tcatccgcaa atggaaaaat atgtggttaa accgatcttc tcccgtgaag
    44041 gcgcaaacgt gtcgatcatt gagaacggca aaaccattga agcagcggaa ggtccgtatg
    44101 gcgaagaagg gatgattgtt cagcaattcc acccgttacc gaaattcggc gacagctata
    44161 tgctgattgg tagctggctg gtgaacgatc aacctgccgg aattggcatt cgtgaagacc
    44221 gtgcgttgat cacccaggat atgtctcggt tttatccaca tatttttatt gagtaatcaa
    44281 tgatgccgga tggcattcgc catccggtaa ttgttaacct atctgcaccg acagcatact
    44341 caggctgccc atttctatac catcgaccgg aatggtaatt ggctcctgcc catcccacgc
    44401 acctaagaca tacaacaacg gcagatagtg ctccggcgtt gggttcgata acgcgccacc
    44461 ttcatggtca aggtaattca ccagaggatg ctgctctacc gccccttgcc acgtcagatt
    44521 cgctttcaca tactcattaa acgacatcgc ccaaggatat ggtgaactat caccgtgcca
    44581 cttcactgta cgcaggttat gcaccacgtt gccactggcg accaacatta ttccttcatc
    44641 tcgcagcgct gccagtttgc gccccatttc gaaatgccag gcggcaggtt tgctactgtc
    44701 gatactcaac tgcaccatcg ggatatcggc gtcaggatac atcttaatca gcacgcccca
    44761 ggaaccgtgg tcaaagcccc aggcttcttt atccagcgtc accgggaccg acgctaacag
    44821 ctcaaccaga tgctgtgcca gcacaggcga acccggagca ggataatgcg tatcgtacag
    44881 cgcctgcggg aagccaccaa agtcatgaat cgtgggcggc gtctccatcg cggtcactcc
    44941 tgttccacgg gtaaaccagt gagccgaaac caccacaatc gcttgcgggc gtggcaatgt
    45001 catccccaac ttctgccagc tgcgggtata caaattatct tccagcacgt tcatcggact
    45061 accgtgacct aaaaacaatg ctggcatacg tgttgaagac atgatgatat ccttaactaa
    45121 aggtgtcatt ttgatatcct cacaatacgc ttgttcggcg gagtaagaac ccggataaca
    45181 atgatgatga tcatcagtta ttttgacgat ctgcctgaag gtgaagattt ataaggagtt
    45241 gtcgatgtca gtacctctca ttctgaccat actggcgggg gcagccacgt ttattggcgc
    45301 gtttctcggc gttctcgggc aaaaaccctc gaaccgctta ctggcgtttt cgctaggatt
    45361 tgcggcgggg atcatgttgc tcatctcatt aatggaaatg cttcctgccg cactagcagc
    45421 tgaaggaatg tcgcctgtgt tgggttatgg aatgtttatc ttcggtctgc ttggctattt
    45481 tggcctggac cgcatgttgc cacatgcgca tccgcaggat ttaatgcaaa aatcggtgca
    45541 gccgttgcca aaatcgatca agcgcacagc cattctgctc actctcggca tcagtctgca
    45601 taacttcccg gaagggattg ccacctttgt cacggcgagc agcaacctgg agctgggatt
    45661 tggcatcgca ctggccgtcg cgttgcacaa tatccctgaa ggtctggcag tggcaggccc
    45721 ggtttatgcg gcaacggggt ctaaacgcac cgcgattctg tgggcgggga tttctggact
    45781 agcagaaatt cttggtggtg tgctggcgtg gttaatcctc ggtagcatga tttccccggt
    45841 ggtcatggcg gcaatcatgg cggcggttgc aggaattatg gtggcgctct cggttgatga
    45901 attaatgccg ctcgccaaag agattgaccc taataataac cccagctatg gcgtactatg
    45961 tggaatgtca gtgatgggat tcagtttagt gctgctacaa acggcgggaa ttggttaaaa
    46021 acaacatcag aagttgtcca gcacatggac aacttctgcc agcttcacga aagccttatt
    46081 ctaccttttc ggctgtctct tctctcgtac tgtttaagta tttgttccgc gcttcaagca
    46141 gtgcgtctct gtcctggcga aacgcccgac aataacgttt caaatgcagg aaataaccca
    46201 ccacaaccag agcaatcaat acaatccagt accaggcgat aaacatcctt ttacccttaa
    46261 tatttaacaa tactaaacat ttagcgcata aatttcacat atcctttttc ggatatattc
    46321 atatggtcgc ataataacgg aacaattata tgattttgat cataatgaaa aagaatgcct
    46381 gatattaatg aaattttctc atagaaagag aagcggaatt aacatcttgc atagcagggc
    46441 aaaataaaaa ccgaagccag ctacaacttc ggtttcttga ttaaggcagt aattaagcag
    46501 cggttttcag ctggctttac gctcatgtgc ctgacggtat gccaccaggt cttcaatagt
    46561 cacgagcgcc atattgtgtt tattggcaaa ctcaatgcac tctggtgcac gcgccatcgt
    46621 gccatcgtca ttagtcagct cacacagcac accagccggt ttaaagcctg ccagcgtcat
    46681 caaatcaata gtcgcttcag tatgaccgcc acgcgtcagt acaccacctg cctgcgcgcg
    46741 aagtgggaaa acgtggcccg ggcgattcag atcagaaggt tttgcgccat cggcaatcgc
    46801 tgcgcgaacg gtcgtaatac gatcagcggc agaaacaccg gtagtcacac cttcagccgc
    46861 ttcaatggtc acggtaaaac cggtgccata ggcgctggtg ttattttcta ccatcattgg
    46921 cagatcaagt tgtttacggc gatcttcagt gatgcacagg caaacaatac cgctaccgtg
    46981 gcgaatggtc agcgccatct gctcaacggt catggtttct gccgggaaga tcatatcgcc
    47041 ttcgttttca cggtcttcat catcaagcac cattacaccg cgtccttcac gcagcgcagc
    47101 cagtgcattt tcaacacgtt cgaaaggcgt accaaaagag gaaagtagcg tctgattcat
    47161 ggtaaaaaaa cctcactaaa attatggtta ccagaatcag ggcagtctta ggagtggcgg
    47221 catatagcca aaataacgtg agcgggtcca tgcccgacag aatcgttact ctctcccatc
    47281 cggactctaa ccgtcggccc cggaattaca ccggatctgc tgtcctttga gttcgcaccc
    47341 aaagcgctcg cgggctttca actgagttga tttaccgccg gtggggaatt tcgccccgcc
    47401 ctgagaataa gcggattcac tataacgcta atgattagcg gcagcaacgc atagcttcac
    47461 ataattctgg tttatgactt acccttatcg cactacaatg gtactcaaca cctatcagta
    47521 cagggaaact acaatgattg acccgaaaaa aattgagcaa atcgctcgcc aggttcacga
    47581 atcaatgcct aaaggaatca gggagttcgg ggaagatgtg gagaaaaaaa tccgccaaac
    47641 cctacaagct cagctgacgc gcctcgacct tgtaagccgt gaagagttcg acgtccaaac
    47701 gcaagtcctg ttacggactc gtgagaaact ggcgcggctt gaacaaagaa tgagcgaact
    47761 ggaaaatcgc agtactgaga ttaaaaagca gccagatcca gagactctcc ccccaacgct
    47821 gtaaacattt gtctttaatg cactgtaaat tatgaattaa tcttctaatt tacagtgctt
    47881 tctattacct tatcgcttaa ttccattcag aatatcatca caaaaatgaa tgagtgcaat
    47941 tgaaaatata attgttatgt attttttaag ttttacttaa tccatattta gataaactaa
    48001 tttcataaaa cccgaaaagt aaaatataat atttgaatga ttagcaatcc ttaaataaca
    48061 gttactaatc attcattttc tacttatatt tatataaagg aatattatgt ctgcttttaa
    48121 aaagtcactt cttgttgctg gcgtagcaat gattctttcc aataacgtat ttgctgacga
    48181 aggtcacggc attgttaaat ttaaaggtga agttatctct gctccatgtt caattaaacc
    48241 aggtgacgaa gacttaactg taaatctggg tgaagtagca gataccgttc tgaaaagcga
    48301 ccagaaatct ttggctgagc ctttcactat tcacctgcaa gactgcatgt tgagccaggg
    48361 tggaaccact tattcaaaag cgaaggttac tttcaccaca gcgaatacta tgacgggtca
    48421 gactgacctg ctgaaaaaca ccaaagaaac tgaaatcggt ggtgccacag gcgttggcgt
    48481 acgtattctg gatagccaga gcggcgaagt gactttaggt actccggtag taattacatt
    48541 caacaatact aacagctatc aggaactgaa cttcaaagct cgtatggaat ccccgggcaa
    48601 agatgctacc ccgggcaacg tttacgctca ggctgactat aagattgctt acgagtaatc
    48661 acattaggcc gggttcgccc ggccacatca ttaacggatt aatgataagt ggaacagatg
    48721 tataaaaaat taaaattaac cacaataagc gaattgatta aaaatattta ttgttcatta
    48781 tccgttctca tcattggttg tgcgtcagct tatgccgttg aattcaacaa agatttaatc
    48841 gaagccgaag atcgtgaaaa cgttaacctt tcccaatttg aaactgatgg ccaattaccc
    48901 gtaggtaaat actcattaaa cgctcttatt aataataaaa ggacgccaat ccatctggac
    48961 ctgcaatggg tattaattga taaccaaact gcagtttgtc tgactccaga gcaattaaca
    49021 ttattaggct ttactgatga gattattgaa gaggctcagc aaaacctgat cgatggttgt
    49081 taccctatcg aaaaagaaaa acaaattaca acttatctcg ataaagggaa aatgcaatta
    49141 tccatatctg cacctcaggc atggttaaaa tacaaagatg caaactggac gcctcctgaa
    49201 ctttgggatc atggtattgc tggggcattt cttgactaca atttatatgc ctctcattat
    49261 gcaccacatc agggcgataa ttcacaaaat ataagttcct atgggcaggc tggggttaat
    49321 cttggggcct ggcgcctgcg tactgattac cagtacgatc agtcatttaa caatggcaaa
    49381 agccaggcga acaacctgga ttttccgcgt atttatttgt ttcgcccaat cccagcaatt
    49441 aatgcaaaac taactatagg tcaatacgat actgaatcct ctattttcga ctctttccat
    49501 ttttctggcg tttcgttgaa aagcgatgag aatatgttgc cgcctgatct acgtggttac
    49561 gcaccgcaaa tcacgggtgt tgcacaaacg aatgcaaagg tcactgtctc acagaacaac
    49621 cgtattattt atcaagaaaa tgttcctcct ggcccatttg ctattactaa tttattcaat
    49681 acattacag
//
LOCUS       DAJVRF010000010        46444 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJVRF010000010 DAJVRF010000000
VERSION     DAJVRF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.010"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..674)
                     /locus_tag="OQN13_000555"
     CDS             complement(<1..674)
                     /locus_tag="OQN13_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HCS1614017.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="OQN13_000556"
     CDS             complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="OQN13_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCS1614018.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="OQN13_000557"
     CDS             complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="OQN13_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCS1614019.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="OQN13_000558"
     CDS             complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="OQN13_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCS1614020.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="OQN13_000559"
     CDS             complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="OQN13_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCS1614021.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="OQN13_000560"
     CDS             complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="OQN13_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HCS1614022.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDNWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="OQN13_000561"
     CDS             complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="OQN13_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCS1614023.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="OQN13_000562"
     CDS             complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="OQN13_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCS1614024.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8400..9752)
                     /gene="hyfD"
                     /locus_tag="OQN13_000563"
     CDS             complement(8400..9752)
                     /gene="hyfD"
                     /locus_tag="OQN13_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCS1614025.1"
                     /translation="MGVLFAALTTLCMLSLISAFYQADKVAVTLTLVNVGDVALFGLV
                     IDRMSTLILFVVVFLGLLVTIYSTGYLTDKNREHPHNGTNRYYAFLLVFIGAMAGLVL
                     SSTLLGQLLFFEITGGCSWALISYYQSDKAQLSALKALLITHIGSLGLYLAAATLFLQ
                     TGTFALSAMSELHGDARYLVYGGILFAAWGKSAQLPMQAWLPDAMEAPTPISAYLHAA
                     SMVKVGVYIFARAIIDGGNIPHVIGGVGMVMALVTILYGFLMYLPQQDMKRLLAWSTI
                     TQLGWMFFGLSLSIFGSRLALEGSIAYIVNHAFAKSLFFLVAGALSYSCGTRLLPRLR
                     GVLHTLPLPSVGFCVAALAITGVPPFNGFFSKFPLFAVGFALSVEYWILLPAMILLMI
                     ESVASFAWFIRWFGRVVPGKPSEAVADAAPLPGSMRLVLIVLIVMSLISSVIAATWLQ
                     "
     gene            complement(9728..9838)
                     /locus_tag="OQN13_000564"
     CDS             complement(9728..9838)
                     /locus_tag="OQN13_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HCS1614026.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="OQN13_000565"
     CDS             complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="OQN13_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCS1614027.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="OQN13_000566"
     CDS             complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="OQN13_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HCS1614028.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="OQN13_000567"
     CDS             complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="OQN13_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCS1614029.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="OQN13_000568"
     CDS             complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="OQN13_000568"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCS1614030.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14171..14743)
                     /locus_tag="OQN13_000569"
     CDS             complement(14171..14743)
                     /locus_tag="OQN13_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCS1614031.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14889..15767
                     /gene="dapA"
                     /locus_tag="OQN13_000570"
     CDS             14889..15767
                     /gene="dapA"
                     /locus_tag="OQN13_000570"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCS1614032.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15784..16818
                     /gene="bamC"
                     /locus_tag="OQN13_000571"
     CDS             15784..16818
                     /gene="bamC"
                     /locus_tag="OQN13_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCS1614033.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17031..17744
                     /gene="purC"
                     /locus_tag="OQN13_000572"
     CDS             17031..17744
                     /gene="purC"
                     /locus_tag="OQN13_000572"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCS1614034.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17912..18760
                     /locus_tag="OQN13_000573"
     CDS             17912..18760
                     /locus_tag="OQN13_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCS1614035.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18775..20790
                     /gene="tmcA"
                     /locus_tag="OQN13_000574"
     CDS             18775..20790
                     /gene="tmcA"
                     /locus_tag="OQN13_000574"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCS1614036.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20864..21562
                     /gene="ypfH"
                     /locus_tag="OQN13_000575"
     CDS             20864..21562
                     /gene="ypfH"
                     /locus_tag="OQN13_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1614037.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21672..21872)
                     /locus_tag="OQN13_000576"
     CDS             complement(21672..21872)
                     /locus_tag="OQN13_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCS1614038.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="OQN13_000577"
     CDS             complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="OQN13_000577"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCS1614039.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23031..23387)
                     /locus_tag="OQN13_000578"
     CDS             complement(23031..23387)
                     /locus_tag="OQN13_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCS1614040.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23769..23828
                     /gene="ypfM"
                     /locus_tag="OQN13_000579"
     CDS             23769..23828
                     /gene="ypfM"
                     /locus_tag="OQN13_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCS1614041.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(23927..27040)
                     /gene="acrD"
                     /locus_tag="OQN13_000580"
     CDS             complement(23927..27040)
                     /gene="acrD"
                     /locus_tag="OQN13_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCS1614042.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(27204..28904)
                     /gene="narQ"
                     /locus_tag="OQN13_000581"
     CDS             complement(27204..28904)
                     /gene="narQ"
                     /locus_tag="OQN13_000581"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCS1614043.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSPPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            29110..31089
                     /gene="aegA"
                     /locus_tag="OQN13_000582"
     CDS             29110..31089
                     /gene="aegA"
                     /locus_tag="OQN13_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCS1614044.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMVFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            31157..31732
                     /gene="nudK"
                     /locus_tag="OQN13_000583"
     CDS             31157..31732
                     /gene="nudK"
                     /locus_tag="OQN13_000583"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCS1614045.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            31858..32901
                     /locus_tag="OQN13_000584"
     CDS             31858..32901
                     /locus_tag="OQN13_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCS1614046.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(32997..35000)
                     /gene="tkt"
                     /locus_tag="OQN13_000585"
     CDS             complement(32997..35000)
                     /gene="tkt"
                     /locus_tag="OQN13_000585"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS1614047.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(35020..35970)
                     /gene="tal"
                     /locus_tag="OQN13_000586"
     CDS             complement(35020..35970)
                     /gene="tal"
                     /locus_tag="OQN13_000586"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCS1614048.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            36259..38538
                     /gene="maeB"
                     /locus_tag="OQN13_000587"
     CDS             36259..38538
                     /gene="maeB"
                     /locus_tag="OQN13_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCS1614049.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            38831..39166
                     /gene="eutS"
                     /locus_tag="OQN13_000588"
     CDS             38831..39166
                     /gene="eutS"
                     /locus_tag="OQN13_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCS1614050.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGVIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            39179..39658
                     /gene="eutP"
                     /locus_tag="OQN13_000589"
     CDS             39179..39658
                     /gene="eutP"
                     /locus_tag="OQN13_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCS1614051.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            39633..40334
                     /gene="eutQ"
                     /locus_tag="OQN13_000590"
     CDS             39633..40334
                     /gene="eutQ"
                     /locus_tag="OQN13_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HCS1614052.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            40331..41134
                     /gene="eutT"
                     /locus_tag="OQN13_000591"
     CDS             40331..41134
                     /gene="eutT"
                     /locus_tag="OQN13_000591"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HCS1614053.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            41131..42147
                     /gene="pta"
                     /locus_tag="OQN13_000592"
     CDS             41131..42147
                     /gene="pta"
                     /locus_tag="OQN13_000592"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCS1614054.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            42186..42479
                     /gene="eutM"
                     /locus_tag="OQN13_000593"
     CDS             42186..42479
                     /gene="eutM"
                     /locus_tag="OQN13_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCS1614055.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            42586..42873
                     /gene="eutN"
                     /locus_tag="OQN13_000594"
     CDS             42586..42873
                     /gene="eutN"
                     /locus_tag="OQN13_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HCS1614056.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            42885..44288
                     /locus_tag="OQN13_000595"
     CDS             42885..44288
                     /locus_tag="OQN13_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HCS1614057.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            44299..45135
                     /gene="eutJ"
                     /locus_tag="OQN13_000596"
     CDS             44299..45135
                     /gene="eutJ"
                     /locus_tag="OQN13_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HCS1614058.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            45125..46312
                     /gene="eutG"
                     /locus_tag="OQN13_000597"
     CDS             45125..46312
                     /gene="eutG"
                     /locus_tag="OQN13_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HCS1614059.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
BASE COUNT        11273 a        12226 c        12644 g        10301 t
ORIGIN      
        1 aaatggcgaa tggcaatggc gaacggaatc acaaacaaat tggcgataca gtgctcaaac
       61 ccactggcga caaacagggt gatgggcaaa atcatggcga cgattttgtc gcataatgaa
      121 cgcccgcaat aactcatcca cagcgccaga caaaccatca gattgcacat aatgccgagg
      181 ctgacagatt cagtaaatgt atgatgcatt ttgccctcgg cgcagtgtaa aaccgcgact
      241 ccccacatcg cgttttcact catcaccagc ccggaaaacc agattaacaa actgaaacag
      301 gcaatacctg ccagattacc gcaggccacc agaagtgcgt taatcagcca agttcgccaa
      361 ctaataacgc cccgactttt tgccatcacc gtcattaccg acgaggtgaa caggctggtg
      421 ccacaaaccg ccagcaaaat aaagccgagt gtaaagcaaa ggccgcccac cagatgggtt
      481 aatgcctgtg aagacggggc atcggcgata acggaaaggt aaaaagtaaa tccaatcgcc
      541 ataaacacgc cagcggacat cgccattaaa aagaccgaca ctttgctgcg ggcaatttta
      601 tgggcagtaa tccgttcagc gattgccgct tttctggcgc tcaactgcaa gtcgaaagag
      661 agtttgtttc tcatgcatac tcctgcaaaa gcagattaca acacctcgcg aaccgagagc
      721 cccagacgct gcattcgtga cagcagcgtg gtgcgcttca tccctaatcg tgtcgccgcg
      781 ccacggggcc cggcaacaat gccattggtt tctcgcaata cctgaacaat gcgctgacgt
      841 tcttcttcgt cattctctgg cgtcgtcggg tgcagcaact gcgccattga actgcgaagc
      901 gctgaatctt catttagcgt cggcagtaaa tggctttgtc ggacattaag atgtaaattc
      961 agactgttac cgcgagtcag cagtaccgct cgctcaatca cgttttccag ctcgcgcacg
     1021 ttgcccggcc agtcccacga catcaactgg cgtagcgcct cggtcgggat ggcgtcaatt
     1081 gaacgattca tatggcgcgc cattttttgc gtgaaatgct ttgctaaaag agggatatct
     1141 tccggacggt cgcgtagcgg cggcaattcc agtgggaaga cattcaggcg ataaaagaga
     1201 tcgctgcgaa actggcgatc ttcaaccatt tgccacaaat cacggttggt ggcggcaatg
     1261 actctgacat tcaccgggat cgttctactc ccgccgagac gctcaatctc ccgctcctgc
     1321 aatacgcgca gcagtttagg ctgaagttct aacggcagat cgccaatttc atcaagaaac
     1381 aacgtgccgc catcggcaat ttcaaaacga ccacgatggg tattaatcgc accagtaaac
     1441 gcccctttgt catgaccgaa taactcactt tccagaagac tggcggggat ggcagcgcag
     1501 ttgattttga ccagcggctt gtcgcgtcgc gggctaagtt gatggatcgc tctggcgatc
     1561 acctctttgc cggttccggt ttcaccacaa atcaacactg tactgtcgct cttcgccaca
     1621 atatctacct gctggagcag gtcttccatt gcctggcttt gatagataat gtcaccgacg
     1681 cccagattcg aaaggagctg ctcgctaagc tggtggtttt cttgctgcaa actttcctgc
     1741 aaatcggtca tgctacgcca ggcatcggca ttgccaacgg caatagcgat gcgatcggct
     1801 atgtgttgta gtagctgaca gttttcctca ctaaagagag tggaagaggt atgcgccagc
     1861 aacaatgcac ccggtgtatg gttgccaaag gtaagcggta taaggagcgc agattcgcag
     1921 ccgttagatg caagtaagag taataacggg tcgcgttgcc agagaaacag atcgtctgca
     1981 cggtgcgtca aggtcggttg tcgggtttgt agcgtttgtg tcagcaatac actgtcgcca
     2041 ggcatattgc gtggcagaca cgcacaatgt gaggcagaaa gatcgctgca ccacaggcta
     2101 aacttctcgt tctttcgatg atcgcccagt accatactga ctgaagccag accgaaaaaa
     2161 tgatggatct cacgagcgac gtcagcgatc agatcgtcga gatcaagatg tgacagcact
     2221 gcattagtaa tatcaaccag tacccgatga tgatcgcgat ctttctccag caaacgcagc
     2281 gccacctgct gttcttcatc attgagccat gattgcagaa ccggggtgat tatccgcgcc
     2341 agccagttaa acagtgtgca atcctgggga gtaaaacggc agccgtgggt gcgaataaag
     2401 cgtagctccg ccattccact actgccgaga aggacggcgt gacgcgaata atcgaccatc
     2461 tcgccctgat actctgttgt tgccgtcaca tcgctgccat catcatggta gcggtaatag
     2521 aagttgcttt tgagaacatg cagttcaata agttgtaccg ggctgaacgg cggcggcagc
     2581 gggatgaagg cgcgtaataa agacgcctta ccgtgtttct gcgctaaccg ctccgcgagc
     2641 attccgttta ccgcttcaat tgttattcct tgcggcgggg caaacatcgc ctcgtctgac
     2701 atagccatca acagtcctta ttaatgagaa atccttctca cgcaattttt ctagccatca
     2761 gatagatggc cggttcctgc acaatttcat ccaacagatg aatgaattga atgctacgcg
     2821 cctgccacac agacgtcgcg tcatccgcca gtggcgcgaa tgcccgggtc aacagggcgg
     2881 tgtggctggc gtcaataaca atctcaccaa aagtcatcac ccccagcatc ttacgtcggg
     2941 cttgctcctc ttcgaccagt gcaagccaat cttcgtattc cgtcagtggg caggggaagg
     3001 cgacattcag acaatcaatc acgccaacgt gatgaccgat agccagcgag taatacatta
     3061 cctgagagct gtgttccggc atctcgtcgc tactggcgac aaactttttt cgcagcgtcc
     3121 agaaaacaat ttccccgcac tcttcagtca tgtaaccccc ggagtacgcg aaagagttgc
     3181 tgaacgatac tattgagacg tggatcgtcg gcatcgcgca accaggtgtt aacccgattt
     3241 cctgtaggat cgtcgcttaa atggcgcagg agccgatcgc aaatttctcg cccctgacga
     3301 tagcccgcca gccgccgcgc ttctcgctca atggcgatac gctgtgatgg cgggatctgc
     3361 ggccacaacg gttgcatagt caccccggtg ggatcgcgat aatccaccgc gtgaatcttc
     3421 tgttgcagca aaccgagcgc cacggcgaaa ccgtgaatgg tggcggccgg tgttggcggg
     3481 cagccgggga tccaaacatc aatggggaca atggtgtcgc tgccgcccca gacgctgtag
     3541 agatcgtgga aaataccgcc gccgacaccg cacgcgccgt aggaaacaca aattttatga
     3601 tcgggggcag actcatacgc ccgaagtgca ggcatacgca tcgcccgggt gactgcgcct
     3661 gtaaataaca aaatatcggc gtgacgcggt gatgaaataa ccttaatgcc aaaacgttct
     3721 gcgtcgaata ctggtgtgat ggcggcaaaa atttcgattt cacaggcgtt gcagccgccg
     3781 caatcgacgc gataaacgta agccgagcga cggatatcct gtagcaaatg ctgcttcatc
     3841 ttttgcgttt gctcgtccag cgtgatgggc tggctgacat gttgtgtaag cactggactc
     3901 atagctgctc cttagccacc agcggtacat ctgtatcgtc gttgagcagc gtcgcgcgtt
     3961 gtttgcactc cgggcagacg ctcgcttgcg cccataacat ttcgcggttt tgtggcgcat
     4021 tttgttgctg tgccaacaat tcagcagcca gtgcgacggt tttttgcggg gcaaacgggc
     4081 gttcgcaacg gctgcaacgt tgtagatgga acgtcgcgcg ggtatagaga tcggctttat
     4141 tggtgacggt cagttcaaag ttattggtaa gctggatggc tctggtcggg cacacttctt
     4201 cacaacgtcc gcagtaaata caacgcctca gatagagctg ccaggtgcgc gaattttgct
     4261 ggtcgtcggt ctggatagtc agcgcatttg ccggacaagc acaggcgccg caggcaatac
     4321 attggctggg catcaggtcc ggtttcccgc gaaagcctgg gctgacctcc agtggcgcga
     4381 agggatattt caccgtcgcg gttccggcgc gcataatagt tttcagtaac ttcagcatct
     4441 tctgtcctta cttcagcggc gaacggttac gatcaatgcc gtagcgttcg atctctttat
     4501 acggcacggt ttttgactgg cgcttgcgca catctaccag cgtcacacgg tcggtacagg
     4561 agtagcaggg atcaaggcta ccgataatca gcggtgcgtc agaaacggta ttgccgcgca
     4621 gcatgtaacg caacaccggc cagttggcgt aggtggcggc acggcagcgc cagcggaaca
     4681 atttttggtt atcaccgagc atgctccagt gcacgtcttc accgcgtggc gcttcaacaa
     4741 agcccagcgc gaatgcgtga ggtttatagc taaagccttc ggtcagcagt ggggtatccg
     4801 gcatgttgtc gagggcaaat tccagcattg ccagcgaatc aaacgtctct ttgacacgga
     4861 ccatcacgcg ggagaaaaca tcgccgccgg taaaggtaaa cagtgttttt ggaatattgc
     4921 cgtagtcggc gtaggggtga tcaaagcgca aatcacgggc aaaaccactg ccgcggatca
     4981 gcggccctac agggctataa tcacgggcga tttgtcggtc gagaatgcca atgccctgag
     5041 tgcgttgttc catattcggc gtggcaagca gcatctctac cagctccgac acgtcggcgc
     5101 gcatctcgcg caccagtttc agcgtttgca gacgttgctc tttgagaata tcgcggcgaa
     5161 caccaccaat cagattcaga ccgtaggttt tacgcgaccc ggtcagcaat tccgccattg
     5221 tcatcgactt ttcccgcacg cggaaaaatt gcataaagcc ggtatcaaat ccaacaaaat
     5281 ggcaggagag gccaaggttg agcaaatgac tgtgcagacg ttcgacttcc agcagaatcg
     5341 agcgaatagt atgcgctcgt tgcggcacct caatccccag tgcattttca accgagttgg
     5401 tataggccac actgtgggca aaaccgcaaa tcccacacac gcgatccgat aagaaggtca
     5461 cttcgttgta gcccatccgc gtttctgcca gtttctccat gccgcgatgg acataaaaca
     5521 ggcggtaatc agcatcgaca atttgctcgc catccacaaa caagcggaag tgacccggtt
     5581 catcggaggt gatatgcagc gggccaatag ggatcacccg cgcatcgcta ttgccctcat
     5641 tgataaacgg atacgtttcg gtatcagtcg tcggttcagg gcgcagtcga taatccatcg
     5701 catctttgcg cagcggatgc atatcttccg gccagttatc gggcaacacc aggcgacgct
     5761 gatccggcag gccaaccgga atcagcccgt acatatcgcg aatttctcgc tcgccccaga
     5821 ccgcggcagg gacgcgcggt gtgactgacg gaaactcccg actgtcggca tcgaccagtg
     5881 ccttcaccac gatccagcat ttttcggccc cttccattga aagggcataa taaaccgcgt
     5941 aatgaccgtt aagtgtccgc tcgtcgttgc caaacaggac tggaagccag ccatcatgtt
     6001 gataataaag ataatgtaca acgtcaggca gcagattgat tttcaccgta atggtgacct
     6061 gttcaggcgt ttgtcgctct tcatccagca ccgcgccggg gaactgcgtt ttcagggcgg
     6121 cgagaatcgc ttcgccacga tttgacgatg aattaacgtt cactgtgttt ctccgaaaca
     6181 gatgcggtgc ctgaaggcaa aaaatcatgc caggtgctac gttgtgcagg caggtcgtgc
     6241 gtccctgaga ggactatagt ggaagcgccc gccaggatcc tgatgacagg ttgtggaata
     6301 tgcgttccca tcgccagcat catgaccagc agaatcacca ttggcgaggt ggtcaaccag
     6361 ccgagatcac cccggttaac ggcctgcggc ggtttcgcca ttaacacccg cgctgccatc
     6421 cgtaccaggc ccgccagcac cagcgttaac agcaataaca gcaggacgat aatcagcagg
     6481 tgattacgtg ccagtccggc ggtaatggtc ataaattcgc taagaaaaat gttgaagggc
     6541 ggcatccctg ccagcgccag cgcaccgccg ccaaacagca cggcggtaaa tggcatgatt
     6601 ttgagcatcc cacagacgac gttgagatcg cgcgtgccgt acttgagcag tacattgccg
     6661 gaaccgcaga acagcagcgt ttttgccaga ctgtggttta agatgtgcag cagcgcggca
     6721 aaaattccca gcggcccgcc aatgcctagc gccaccgcga ccagccccat gttctccacg
     6781 ctggagtacg ccagcagacg cttaatgtcc cgctgtacca gaatgaaaaa tgccgccacg
     6841 gcaaccgaca acatgccgaa gatgagcaac aaccggttgg ggaaatcgct gccgatggct
     6901 tggcaaataa tgatgtaata gcgaatcagc accaacagcg cgcagttcag caatacggcg
     6961 gagagcaggg cgctgaccgg actcggcgct tcactgtgag catccggcag ccaggcgtgc
     7021 atgggaaata gcccggtttt ggtaccaaag ccaattagca caaacacaaa ggccaacagc
     7081 attaatgttg ggtcaagcaa ggacgattgc ttaagaacct cgctccagaa tatcgccatt
     7141 tctgcctgcg gcataacgct ggcggcgttg gcgtatacca gcacggtacc gaacagacca
     7201 aaagcgacac caacagtaca aataatgatg tacttccatg ctgattccag cgatgaacgc
     7261 tgaccgtaaa tgcctaccag aaacgccgag cttaaggtgg tggcttcgat cgccgcccac
     7321 atcacaatca ggttattgct ggtaacaacc agcagcatgg tgaacaaaaa cagatggaag
     7381 aaaccgtagt aatcgcacag cgtaacgggt gaaagctcgc cgtgtgccac ttcatgacgc
     7441 atgtagccaa tcgagtaaac accggtgaga aagccaatca caccaagaat ggcgaggaac
     7501 aaaccgccca gaccatcaat atgaagccac agtcccgcag cgaatatttc tcctgcatca
     7561 gcggcagttt ggaccaccca gagtgccaga atcagcagca gtgtgatccc taagctatgt
     7621 aatacagtca ccgtacaagt cgcagagagc ccccgtttcc ggcaggcaaa acagagcagc
     7681 gaaaaaagca gcggcgtgag caggagtaaa gcgaacatca cagaataact catctcatta
     7741 tcccttcagc gcggtcaagt tgttcacgtc cagcgtgccg tgggtacgcc atatttttct
     7801 tgccagtaac accatcacaa tgacggcgaa gatggcgtcg gtagcgatac ctatttccac
     7861 cagttccggt gctcgccagg caagaagcgc cagcaccaga tgggagccgt tttccatcag
     7921 gcagtaacca aaaatttgcc gcaggatatt gcgctggctg acaatgcaca gcaggccaag
     7981 cagaaaatga cctaacgcta ccgccagcgc cggtttcagc ccggtagcca tcggtagctt
     8041 cacgggctga acgacaaatg cgcaaagcag gacaattaac gccgcgagca gtgccatcat
     8101 tgccggaccg aataacgctt tttccgggat gttctgggga atatttcgtg cagcgtaagt
     8161 catgattaac ggtaccagca gcactttggt gataaaggcg ctggcggacc agatcagcag
     8221 ttgctctgcg gcgaacaggc acgagagagt ggcgaaaata gacaccagca ccagcgactg
     8281 gcaggcgtaa aatccgcatg acaggcgata gcttttgacg ctaatcacaa acagcgatgt
     8341 cagcatcatc agtcccgcca gattatttac gatcatagaa ccggtcattc atcatctcct
     8401 tactgcaacc aggtcgcggc gattacgctg gaaatcagcg acatcacaat cagtacaatc
     8461 aacaccaggc gcattgaccc tggcagcggt gcggcatcgg cgacggcctc gctcggtttg
     8521 cccggcacga cgcgaccaaa ccagcgaata aaccaggcga aactggcgac cgattcaatc
     8581 atcagcagaa tcatggcggg cagcaggatc cagtactcca ctgacaacgc aaaaccgacg
     8641 gcaaacagcg ggaatttact gaagaagccg ttgaacggcg gcacaccggt aatcgccagc
     8701 gctgccacgc agaaacccac gcttggcaac ggcagggtgt gcaatacgcc acgcagacgc
     8761 ggcaacaagc gcgtgccgca gctgtaactc agcgcacctg ctacaaggaa aaacaggctt
     8821 ttagcgaacg cgtggttgac gatgtaggcg atgctaccct ccagcgccag ccgcgagccg
     8881 aagatggaga gcgacaagcc gaagaacatc cagccaagtt gagtgatggt cgaccaggcc
     8941 agcaaccgct tcatatcctg ctgtggcaaa tacatcagaa agccataaag aatggtgacc
     9001 agcgccatga ccatgccaac gccgccaatc acatgcggga tattgccgcc gtcgataatg
     9061 gcgcgggcaa aaatgtaaac gcccactttc accatcgatg cggcgtggag ataggcactg
     9121 atcggtgttg gcgcttccat tgcatccggt agccacgctt gcatcggtag ctgggccgat
     9181 ttcccccacg cggcaaacag gatgccgcca taaaccagat aacgtgcgtc gccgtgtaac
     9241 tcgctcatcg cgctaagcgc aaacgttccg gtctgcaaaa acagcgtggc ggcggcaaga
     9301 tacaacccca acgagccgat atgagtgata agtaacgctt ttagtgctga aagctgtgct
     9361 ttatcgctct ggtaataact gatcaacgcc caggagcagc cgcccgtaat ttcaaaaaac
     9421 aacaactgac cgagcagcgt cgaggagagt accagtcccg ccatcgcgcc gataaacacc
     9481 agcaaaaatg cgtaataacg attcgtgccg ttatgcgggt gttcgcgatt tttatccgtc
     9541 agataacccg tggagtagat cgtgaccagc aaaccgagaa acaccaccac aaacagaatc
     9601 agcgtactca tgcgatcaat gaccaagcca aacaatgcca catcccccac gttgaccaac
     9661 gtcaacgtga cggcaacttt atcggcctga taaaacgcgg agatcagcga caacatgcac
     9721 agcgtggtca gtgcggcgaa caaaaccccc attcggcggc ccgacgttgt ggcgaaaacg
     9781 aaacgaccag tgcgccgata aaaggcagca ttaacgtcgt cagagcaaga ttttccatat
     9841 tccgtcagtg ctccttacag accggttaac cagaagaccc atgcaagcgc agcaaggctg
     9901 aagccaagcc aggtcacatg gtgaatgagt aaaaaacgcc cgcgtgccag cgtgttttct
     9961 gcgattgagg ccagtacaaa aatcagcaaa accttgagca gcgtaacgac aagtgaagtc
    10021 agcaggcagg cgagagaaag ttcttgcgcg cgcccaaagg gcagaaacag ggccacaaac
    10081 agtgctgcca tcacgacctg tttcagcccc agcccccatt tcgctagcgc cagcccggca
    10141 ccggaatatt cggtcagcgg gccttcctgt aattcctgtt ctgcttcagc aacatcaaag
    10201 ggaatttttc ccatctcaat gaagcaggca aaaccacagg ccagtaacgc cagtacggtg
    10261 gttagcggcg agttccagcc cgttgccagc gtattgctga tcatctcgat atgcgtggaa
    10321 cctgctatca gcgccaatac cagcagtgag agaatgagca tgggttcgac cagaatgccg
    10381 agcgtcaact cgcgactggc accgactccc gcaaacgggc ttccggtatc cagccctgaa
    10441 agagcaaaga aaaaacgaaa cagggcaaga agatagataa gggtgatcag atcgccgccg
    10501 cccgcaaaag gggaaacggt aataaatagt ggtagggcca tcgccagcac cagcatgctg
    10561 ctgattaata cccacggcat caggcggaac atcagacctg aagatgtcgg cgcatcttcc
    10621 tggcgtttaa acagtttgtg gatatcgcga taatcctgcc agatccccgg cccgcggcgg
    10681 gagtgcatac gcgcgcgtat ctgccgggaa atacccgtaa aaagtggggt tagcatcagc
    10741 agaatcacgg cctgtgctaa cgcaaaaatg accagatatc cgtcgcaaag agtttgtctc
    10801 atggtgattt ccttagacgg caatagcgat tagcagcaca accagtgcgg cgaccacata
    10861 caggcaatag agacgaaagt cgccgctttg cagatgctgt atttctttgg ccagccgttg
    10921 ggtggcgctc acgatggggc ggatcacccg ctcatcccag aagggttctg tgctctgagc
    10981 cctggcggtg acgtgcgcaa gacctttatt tagcctcagc gtagggtcga gctgttgtcg
    11041 tagacgaaat agcgcagaaa agaccacacg cagcggctgc atcacgccat tgcctgacgg
    11101 ggccatcgca ttttcccagc cgtagccgca tgcccaggca tctcctgtgc gacgaaacgc
    11161 tgcgcggcgc gaacgacacc acagccagta aaggccaggc agcaaaggca tcgccagtag
    11221 taacagcaac agtaatgaag gagtgacccg tgtatgaaac gtgccgggta caagtgctat
    11281 tccgctggcg acagtgacgg gaggggtatt ggtaaacgca tgggcaatgt gcattatctt
    11341 cggtgccagc caactggcac taagcgcaat taatacgcag agtgccgcga gcagtagcat
    11401 ggcgacgatc atcgtatttg gcacttcctg tgcctcttca gcgtgtgtgc tgcgcggcgc
    11461 accacagaaa gtaataccgt acattttgac gaagcacatt actgccagcc caccagtgac
    11521 tgccagcatt accatagcaa taggacccgc aagttgtagc gctacggctt ccacacgact
    11581 tagtgagaat agcgactgcc aggtgtacca ttcgctgata aaaccattca gcggaggaat
    11641 ggctgatatc gccaggcaac caatcaggca tgctgcggct gtccacggca tccgtttcgc
    11701 cagtgccccc attttttcca tgtcgtgggt atgcaaacgc gaaataatcg cacccgcgcc
    11761 gagaaatagc agccctttga aaagcgcatg gttgagcaga tgaaacagtg cgccgagcag
    11821 tccaaccacg gtgagcagcg ggtcgtgcag tgacagaccg accatcgcca caccgaccgc
    11881 cagcaaaata atgccgacgt tttcgacggt actccaggcc agcagccgtt tgatatcctg
    11941 ttccgccagt gcatacagca cgcccaggag cgcggagatt gcgccgatcg ccatcaccag
    12001 aatgccccac cacagcggca aacccgtttg cgccagcaga tccatcgcca ctttcaggat
    12061 gccgaaaata ccgattttga ccattacgcc agacatcaac gctgaagcgt gcgatggtgc
    12121 ggcagggtga gcgcgcggca accagctgtg caacggcatc atcccggctt tcgcgccaaa
    12181 accgaaaaag gccagcagga acaccgccga cgccagcccc ggagaaagtg aaagcgtgcg
    12241 gaaactggca aaatcgaggc tgccgctttc gcgccccatc agcaagaagg cgatcattat
    12301 cagcaccgat ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga tcgacgtttt
    12361 atcctgcctg gcaatgacca gaaaccagga agacagcgac atcatttcaa acagcacgat
    12421 gaaccaaaaa gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga aaatattcat
    12481 aaagaagccc atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata
    12541 gagagaacaa accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc
    12601 cattcgcagg gttaaatcgg caaattcaaa aggcgtggca aaacgggcga ctaaagttac
    12661 gcctgcatgt aattgcgtaa ttccgctgat gatgccaatc acgccgccca ccagcgatgt
    12721 gatgccggaa agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact
    12781 ggcaaacaga tagaggatca gcgaccaggt taataattgc agggcatcca ttagcgttgc
    12841 tcctgagtga gggaaagggg atcctccccg ccattggcaa accagcttgc ggcaaggcgc
    12901 tgtttttctt tcatctggcg ttgcaggcta tcgtcggtga tcagccgtaa cgcctgattc
    12961 gggcaagcgc gaacgcaggc cggaccttct ggcaagaaat cacacaggtc gcatttcacc
    13021 gcgacggtct ggacacccgg ttcccagcgc agcaacgcat gttggggcgg cacgttttct
    13081 tcgccgtctt tgagtgagcc ctcagcctga aaaacatatt gcgcaggggc attcaccgga
    13141 cggcttcctg aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcagcca
    13201 atacagaggc tttcgttgag ttggatcgca tcatccctct gagagatggc attgaccggg
    13261 cagacctgca ggcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgagtgtt
    13321 gacgtcttcg ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc
    13381 gaacaggcag cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag
    13441 cggttcatgt cggctccttg aaaatgatat gaaacattgc atttcatatt gcataaagga
    13501 tgctattctg attaactcaa tatattcaat aagttatgta ttaactggat tctgaacggt
    13561 acaaaaatga tggtcattaa cgttcagcaa aaaatggatt cgacacccgt gtcgcagggg
    13621 aaagcgagaa tgtgatcttg atcacgagaa gtgaggtatg ctggccgcaa gcgcagccag
    13681 cacggaatgg agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg
    13741 tgattgctgg ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg
    13801 aagctgatgc gatgaatgcc atcgtaggtt ttgcccatga aggacttttc accccagacg
    13861 ccgaattgtt cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct
    13921 ttttccgcaa aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg
    13981 cccgcttttt tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg
    14041 ggggtcatgg ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa
    14101 ttaacttgtt ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc
    14161 agtggattca ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa
    14221 tactgccttg tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg
    14281 cgtccgcaga tgcggggctg tgggcggtta tctgaatatg caactgcgca gcccgttcat
    14341 tttcagcagg ttgcgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa
    14401 gtgctgtgaa gcgttcaatt aaatgcgggg agtctgccac atcgacctga acccagacag
    14461 atgctggcat tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt aaaagatcca
    14521 gctcggcacc tttcaacggt aacgttgatt caatcagagt aatggcattc catgaaccgg
    14581 aaagcagcat gataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat
    14641 tacagccgca actactgaca tgacgggtga tggtgttcac aattccaggg cgatcggcac
    14701 ccaatgcagt gatcaccaga taatgttgcg atgacagtgt caaactggtt attcctttaa
    14761 ggggtgagtt gttcttaagg aaagcataaa aaaaacatgc atacaacaat cagaacggtt
    14821 ctgtctgctt gcttttaatg ccataccaaa cgtaccattg agacacttgt ttgcacagag
    14881 gatggcccat gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta
    14941 atgtctgtcg ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg
    15001 cgatcgtttc tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg
    15061 atgtggtgat gatgacgctg gagctggctg atgggcgcat tccggtaatt gccgggaccg
    15121 gcgctaacgc tactgcggaa gccattagcc tgacgcagcg cttcaatgac agtggtatcg
    15181 tcggctgcct gacggtaacc ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc
    15241 atttcaaagc catcgctgag catactgacc tgccgcaaat tctgtataat gtgccgtccc
    15301 gtactggctg cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta
    15361 tcggaatcaa agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt
    15421 cagatgattt tgttctgctg agcggcgatg atgcgagcgc gctggacttc atgcaattag
    15481 gcggtcatgg ggttatttcc gttacggcta acgtcgcagc gcgtgatatg gcccagatgt
    15541 gcaaactggc agcagaaggg cattttgccg aggcacgcgt tattaatcag cgtctgatgc
    15601 cattacacaa caaactattt gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg
    15661 aactgggtct tgtggcgacc gatacgctgc gcctgccaat gacaccaatc accgacagtg
    15721 gtcgtgagac ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat
    15781 ttgatggctt actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt
    15841 ttattactcg ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa
    15901 gcctacctgg aagcggcacc gcttgcggag cttcatgccc cggctggaat gattttgccg
    15961 gtgacctccg gtgattatgc aatcccggtg accaacggta gtggtgctgt cggtaaggcg
    16021 ctggacattc gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc
    16081 acgggcgata ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag
    16141 gtggttagcg tgctgcaggc gaaaaactac accatcaccc aacgtgatga tgctggtcag
    16201 acactgacca ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt
    16261 cgttatcaaa tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgttg
    16321 aacctggaac aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg
    16381 gagatgatga acgttatttc cgccggtctg gataaatctg ccactgacgc cgcgaacgct
    16441 gcgcaaaatc gtgcctccac cactatggac gtacaaagtg cagctgatga caccggttta
    16501 ccaatgctgg tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agcggcgctg
    16561 gaaaaagtgg gcatgaaagt gaccgacagc acccgttcgc agggcaacat ggccgtaact
    16621 tataagccgc tgtctgacag cgactggcag gaactgggcg cgagcgatcc aggcctggca
    16681 tccggtgact ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc
    16741 gatccgaaag gtcatactct gactcagagt cagaacgacg cgctggtagc tgtcttccag
    16801 gctgcgttta gcaagtaaaa atacagggct ggaatcatcc ggcccttttt tctgatatga
    16861 tacgcaaacg tgtgcgtcgg caggaaaacg cgattttagc ggtaattcgc acgaaatttg
    16921 tttgtcggac gtagttcgga taaggcgttc acgccgcatc cgacaaaaca tccggcacac
    16981 cagacagcaa aagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc
    17041 aagctgagtt gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt
    17101 tggtgctcga attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg
    17161 atcgcaaagg tatgttgaac aacaagttca actacttcat tatgagcaaa ctggctgaag
    17221 cgggtatccc gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc
    17281 tggatatggt gccggttgag tgtgtcgtgc gtaaccgtgc tgcgggctct ctggtgaaac
    17341 gtcttggaat cgaagaaggt attgagctga acccgccgct gttcgatctg ttcctgaaaa
    17401 acgatgccat gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtga
    17461 gcaaagagaa cctggcgcgt atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa
    17521 aactgttcga tgatgctggt ctgattctgg tcgacttcaa gctggagttt ggtctgtaca
    17581 aaggtgaagt ggtactgggc gatgagttct ccccggacgg cagccgcctg tgggacaaag
    17641 aaacactgga gaaaatggac aaagaccgtt tccgccagag cctcggtggc ctgatcgaag
    17701 cctatgaagc cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc tgttcatcat
    17761 cttgccgtgc agctggcacg gcaagacaac cgctctcgga atatatgcat tgaacgctca
    17821 ttcctccgcc atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca
    17881 tttctataat gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga
    17941 caatgtcgaa gacaggcgta acagctccgg cggtccgtca atgggcggtc ccggttttcg
    18001 cctgccaagc ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta
    18061 tggtgttgat ttaaccgggt tgatgaccgg acagccggtt tcccaacagc aatcaacacg
    18121 gtcaattagc ccaaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac
    18181 ggaagacacc tggggacagc aattcgagaa gatgggtaaa acctatcagc aaccgaagct
    18241 ggtcatgtat cgtggaatga cgcgtaccgg ctgcggggcg ggccagtcca ttatgggacc
    18301 gttctattgc ccggcggatg gcacggttta tatcgatctc tccttctatg atgacatgaa
    18361 agacaaactc ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg
    18421 tcatcatgtg cagaaattgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc
    18481 gacgcaggcg gaagtaaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc
    18541 cggtgtctgg gggcataaca tgcagcagca aggcgatctg gaagaggcgc tgaacgcggc
    18601 gcaggccatc ggcgatgacc gtttacaaca gcaaagtcag gggcgagtag taccagacag
    18661 tttcactcat ggtacttctc agcaacgcta cagctggttt aaacgtggtt tcgacagcgg
    18721 cgatccggca caatgcaata cttttggtaa aagcatttaa cttccggggc aaggatggct
    18781 gaactgactg cgcttcacac attaacagcg caaatgaaac gtgaagggat ccgccgcttg
    18841 ctggtgttga gcggggaaga gggttggtgt tttgatcatg cgcttaagtt gcgtgatgcc
    18901 ttacctggcg actggctgtg gatttcgccg cagccagatg ctgaaaacca ctgttctccc
    18961 tcggcgctac aaactttact tgggcgcgag ttccggcatg cggtattcga cgcccgccac
    19021 ggctttgatg ccgctgcctt tgcggcactt agcggaacgt tgaaagcggg aagctggctg
    19081 gttttgttac tccctgtatg ggaagagtgg gaaaaccaac ctgatgccga ctcgctgcgc
    19141 tggagtgatt gccctgaccc tattgcgacg ccgcattttg tccagcatct caaacgcgta
    19201 cttacggcgg ataacgacgc tattctctgg cggcaaaacc agccgttctc gttggcgcat
    19261 tttacccccc gtactgactg gcaccccgcg accggcgcac cacaaccaga acaacagcaa
    19321 ctcttacagc agctactgac catgccgccg ggcgtggcag cggtaacggc tgcgcgtggg
    19381 cgcggtaagt ccgcgctggc agggcaactc atttctcgta ttgcgggcag tgcgattgtc
    19441 accgcgcccg caaaagcggc aacggatgta ctggcacaat ttgcgggcga gaagtttcgc
    19501 tttattgcgc cggatgcctt gttagccagc gatgagcaag ccgactggct ggtggtcgat
    19561 gaagccgcag ccatacctgc gccgttgttg catcaactgg tatcgcgttt tcctcgaacg
    19621 ttgttaacca ctacggtgca gggctacgaa ggtaccggac gtggtttttt gctgaaattt
    19681 tgcgctcgct ttccgcattt acaccgtttt gaactgcaac agccgatccg ctgggcacag
    19741 ggatgcccgc tggaaaaaat ggttagtgag gcactggttt ttaacgatga aaacttcacc
    19801 catacaccac aaggcaatat cgtcatttcc gcatttgaac aaacgttatg gcgaagcgat
    19861 ccagaaacgc cgttaaaggt ttatcagtta ttgtctggtg cgcactaccg gacctcgccg
    19921 ctggatttac gccggatgat ggatgcacca gggcaacatt ttttacaggc ggctggcgaa
    19981 aacgagattg ccggggcgtt gtggctggtg gatgagggcg gattatctca agaactcagt
    20041 caggcggtat gggcaggttt tcgtcgcccg cggggtaatc tggtggccca gtcgctggcg
    20101 gcgcacggca gcaatccact ggcggcgaca ttgcgcggac ggcgggtcag ccggattgca
    20161 gtccatccgg cgcgtcagcg cgaaggcgtt gggcaacagc tcattgccag cgctttgcaa
    20221 tataggcctg gcctcgacta tctttcggtg agttttggtt acaccgggga gttatggcgt
    20281 ttctggcaac gctgcggttt tgtgctggtg cgaatgggta atcatcgtga agccagcagc
    20341 ggttgctata cggcgatggc actgttaccg atgagtgatg cgggtaaaca gctggctgaa
    20401 cgtgagcatt accgtttacg tcgcgatgcg caagctctcg cgcagtggaa tggcgaaacg
    20461 cttcctgttg atccactaaa caatgccgtc ctttctgacg acgactggct tgaactggcc
    20521 ggttttgctt tcgctcatcg tccgctatta acgtcgttag gttgcttatt gcgtctgcta
    20581 caaaccagtg aactggcatt accggcgctg cgtgggcgtt tacagaaaaa caccagtgac
    20641 gcgcagttat gtaccacact taaactttca ggccgcaaga tgttactggt tcgccagcgg
    20701 gaagaggccg cgcaggcgct gttcgcactt aatgaggttc gcactgagcg tctgcgcgat
    20761 cgcataacgc aatggcaatt ttttcactga ctccttcaat tttctcccat ggtcattgtg
    20821 agtcagcggc gtaaaattaa tgacatcagt taaggagttc gccatgaaac atgaccattt
    20881 tgttgttcaa agcccggata aacctgcgca acagttgtta ctgcttttcc acggtgtcgg
    20941 ggataacccg gtggcaatgg gggaaatagg cagctggttt gcgccgctat ttcctgatgc
    21001 gctggtggtg agtgtcggag gcgcggagcc gagcggtaat ccggcggggc gtcagtggtt
    21061 ttcggtgcag ggtattacgg aagataatcg ccaggcgcgt gtggatgcca tcatgccgac
    21121 gtttattgag atggtgcgct actggcagaa acagagcgga gtaggggcta acgccacagc
    21181 gctaatcggt ttctctcaag gcgcgattat ggcgctggag agcattaaag ccgaaccggg
    21241 ccttgcttca cgcgtcattg cttttaacgg acgctatgcg agcctgccgg aaactgcgtc
    21301 tacagccacg acaattcacc tgattcatgg cggtgaagat ccggttatcg atctggcgca
    21361 tgcggttgcg gcgcaggaag cgttaatcag tgccggtggt gacgtgacgc tggatatcgt
    21421 ggaggattta ggccatgcga tcgacaatcg cagtatgcaa tttgccctgg atcacttgcg
    21481 ttataccatt ccgaaacatt atttcgacga agcgctaagt ggcggtaagc ctggcgatga
    21541 tgatgtgatt gaaatgatgt aaaagacaga ttacctggcc tgataagacg cgtgagcgtc
    21601 gcatcaggca tcgttcacca ttgccggatg cggcgttaac gccttatccg gcctacggtt
    21661 gagcactcag cttacttctt cggttgatcc tttttcggcc agtcgtcgtc atcgtcccac
    21721 ttatcgttga aatcacgatg cgggggaagc tccggcttgt tggcgagaaa tttcttgtgg
    21781 tcgacgcgct tgagatcttt aatcacgttc agcaggacgc ctaccaaaaa gacaatcacc
    21841 agaatccacc aatatttagc cagccagtcc atgcttattt cctcttgcag aaccactcat
    21901 caggcgacga gctgttccat gatacgttga tacatacggg caagtagctg caggtcggca
    21961 gcgttcacac attcattaat tttatgaata gtggcattga ccggcccgag ttccaccacc
    22021 tgcgccccca tgcgagcaat aaagcgcccg tcggacgttc cgcctgtggt cagtagctgc
    22081 ggtttaattt cattatagtg ctcaaccgcg ttaacgaccg catccaccag tttaccgcgc
    22141 gcggtcaaaa atggctgccc ggaaagccac caatccaccg tatagcgcag ttgatgtttt
    22201 tcaagcaggg caagcacctg cgctttgatc atctcatcag tcagttcggt gctgaagcgg
    22261 aagttaaact gcacaaacag ttcacccgga ataacgttgt tactgcccgt tcccgcctga
    22321 atattggcaa tctgcatact ggtcgccggg aagaattcat tgccctgatc ccactcaata
    22381 gccactaatt cattaaggaa aggtgctgcg cgatgtactg gattgtcagc cagatgtggg
    22441 taggccacat gcccctgcac gccatgaatg gtaaggttgc aggttaacga tccgcgacga
    22501 ccatttttca ccacatcacc aaccacttcg atactcgacg gttcgccgac cagacagtaa
    22561 tcgagacgct cattacgcgc cattaacgct tcgacgactt ttaccgtacc gttgtgggca
    22621 ctggcttctt catcagaggt gatcagaaat gccagtcgtc ccgtatggtt gggatgttgt
    22681 gcgacaaaac gttctgctgc taccaccatc gccgccagcg agcctttcat atctgccgca
    22741 ccgcgcccga ataacatgcc atcacgaatg gtgggttcaa acggcggatt gatccaacga
    22801 tcggcgtcgc caggcggcac cacgtcggta tgcccggcaa aggccaatgt ttcaccttgc
    22861 ccacgccatg cccaaaaatt ctgcgtatcg gcaaagtcca tgcgttcaac ggtaaaaccg
    22921 atcgcctgca aacgatcaat caacaaagcc tggcatcctg cgtcatcagg actcagggaa
    22981 gggcggcgaa taagctgttg tgtcagctca ataaccgggc acgacataga ctacacctca
    23041 tggaaaaatt gctgataact ggaatcactg aaacccagca gcataggctt accgggcgcg
    23101 cagagcaatg gacgtttgat aattgcaggc atttcagtca ttaatgccgc cgctgaggcc
    23161 gcatcggtga ttttattgcg ggtggtttcg tccagtttac gccaggttgt accacgggtg
    23221 ttgagtaacg cctcccaacc taattcgttg ataaaaccgt tcaataattc gctgtccagc
    23281 ccatcgacgc ggtaatcatg aaaacgatag tcgatgttat tggcttccag ccaacggcga
    23341 gcctttttaa tggtgtcaca atttttgata ccgtaaagtg taaccataat gaatcctttt
    23401 tcgatttatg agaataaata ctcatttaag ggtgaaaaca ttttgcctgc aaatgattat
    23461 caggaaatat aacattatcg aagtattcaa attaacaact attaaataat attaaacatt
    23521 tttaacgtaa acattaagaa tattcatctt gttatctgtc tgtattttaa cctgaattaa
    23581 atcagtatta gatgccgtat gaatatctcg aaatgttgct gtgtatcaca tacaaatcat
    23641 caccgcagga ccatagtatt tacataagat ttgtcccccc ggaggtagtc atcgcagcaa
    23701 tcgagtaaat tagcttcacc acggtatatt gtttccgtag aatacccata attataagag
    23761 aggttgttat gattgaacgt gaactgggga actggaaaga ctttatcgaa gttatgcttc
    23821 gtaagtaatt cctgagacaa agtataaaca gcaagaaccc gctgagcagg ttcttaatcg
    23881 gaaagttgaa aaaggcgaca cattggcatg tcgccttttt tattgcttat tccgggcgcg
    23941 gctttagcgg gaagcggcgg cgcaccagca caaagaacag cggcacgaag taaatagcca
    24001 gaatagtggc tgaaatcatc ccgcccatta cgccagtacc caccgcatgc tgaccaccgg
    24061 aaccggcacc cgtgctggtt gccattggca atacgccgaa gataaatgcc agcgaggtca
    24121 tcagaatcgg gcgtaaacgc tgacggcagg cgtggagcgt cgcttcaaac aggtcgtggc
    24181 ctttttggtt catctcgtta gcaaactcga cgatcaggat ggcgtttttc gccgataaac
    24241 caatgaccgt taacaggccc acctggaagt aaacgtcgtt ttccagcccg cgcatccagg
    24301 ttgccagcag cgcgccgatt acccccagcg gcacgaccag cattaccgag aacggcaccg
    24361 accagctttc atacaacgca gccagacaca ggaataccac cagcaaggaa atggcgtaca
    24421 gcgccggagc ctgcgcgccg gaaagtcgct cctgatacga catcgccgtc cactccagac
    24481 caaagccgtt tggcagctgc ttcactaacg attccataat atccatcgcc gtaccggtac
    24541 tgacccccgg tgcggcttcc ccaacaatct caaccgcaga ataaccgtta tagcgttcca
    24601 gacgcggcga gcctgtttcc cagcgtgagg tcgcgaaagc agagaagggc accatgccgc
    24661 catctttatt tcggacatac cagagattga tgtcatctgg cagcatgcga tacggcgcag
    24721 ctgcctgcac atagactttc ttcacgcgac cgcgatccat aaagtcattc acatagctcg
    24781 aaccccaggc ggtttgcagt gtgtcgttaa tatcgtcgat agcaacgccc agcgcctgag
    24841 ctttacgctg gtcgatatca atctgcaact gcggactgtc gtcgaggccg ttatggcgca
    24901 cacgggttag ctccgggttt tccgccgcca gcgccagcaa ctggttacgt gctgccatca
    24961 gcgcatcgtg acctgctcca gcgtggtcct gcaactccat atcaaaaccc gcagaactac
    25021 caagaccgct aattgctggc gggctgctgg cgataacgcg agcttcttta atcttgttaa
    25081 acgcctttgt tgcacgctcg ataatggcaa acgaggtgcc ggtcttactg tcgcgttcgc
    25141 tccagtcttt caggcggata aacattcgcg ccacgttttg cccgttaccc ccagggccag
    25201 aaccaacggt ggcaaacacc gacatgatgt tgtctttttc atgggtgaag taatatttct
    25261 cgatttgctc aacgactttc agggtctgtt gttgggttga accgctgggc aattgtaccg
    25321 aggtagtaaa catgccacgg tcttccagcg gtaagaacga cgtcggcaaa cgcaggaaca
    25381 ggaacaccat gccgccaagc agcaggacat aaatcacaat ccagcgcagg ctgcggtgga
    25441 gaattttcgc cacccctttt tcgtagcgtt cggcgttgcg gttaaacatc tggttaaacc
    25501 aggcaaaaaa gcctttttgc ccgtgatgct cacctttttt cagtggctta agtagtgtgg
    25561 cacacagagc cggagtgagg atcatcgcta ccagtactga cagcaccatc gccgcaacaa
    25621 tggtaataga gaactggcga tagatggcac cggtggtgcc gccgaagaag gccattggta
    25681 caaataccgc cgaaagcacc atcgcaatcc cgaccagtgc cccctggatc tgccccatcg
    25741 atttacgtgt ggcttcgcga ggagtgagtc cttcctcact cataatacgt tcgacgtttt
    25801 ccaccaccac gattgcgtca tccaccagca gaccgatcgc cagtaccatc gcgaacatgg
    25861 ttaaggtgtt gacgctgtaa ccgaaggcgt aaagtacgga gaaggttccc atcaacacca
    25921 ccggcacggc gatagtgggt atcagcgtgg cgcggaagtt ttgcaggaac agatacataa
    25981 cgaggaaaac cagggcgata gcttccagca gcgttttcac cacgtcttca atggaggctt
    26041 taacaaacga ggtggtttca tacgccacct tgtattccag tccgtgcggg aagtactgcg
    26101 ccagctcgtc gagacgattg agcaccagct ccgctgtcgc catttcgttt gcgccggagg
    26161 ccagttttac ccccagcccg gaggctggct taccattgaa gcggctaaga taatcgtatt
    26221 tctccgcccc catttcgacg gtggcgacat cgcccagcct tacctctgag ccgtcttgat
    26281 tcacccgcaa ggtgatatcg cggaactgtt ctggtgtttg cagcagtgac tgggcgttaa
    26341 tggtggcgtt gagcgcctgc ttatcgacgg aaggtgtacc accaagttgc ccaaccgcaa
    26401 tctgcgcgtt ctgtgactca atggcatcag tgacatcttt agccgtcatc tggaaactgt
    26461 tgagtttcgc cgggtccagc cagatacgca tggaatattg cgaaccatag gcatcgatat
    26521 ccccgacgcc attcacgcgg cttaacgggt cctgaatatt actggcaaca taatcggcaa
    26581 tatcctgttt atccatcgaa ccatcggtag agacgaaagc aatggtcaga atattggtat
    26641 cgccggtttt acgcaccgtc acgccctgat tttgcaccgc ctgcggtaac tttcgcatgg
    26701 ctgattgcag ctggttttgt acttgctgca cggcttcgtc cggatcggtg cctgctttaa
    26761 aacttaaagt gacagatgcc tgaccgatgc cactgctctg agatgacata tacatgagat
    26821 tatcgaggcc ggtcatattt tgctcgataa cctgggtcac ggtgttttcc agcgtctggg
    26881 ccgatgcgcc gggatagtta gcggtcactc gcacattcgg tggcgcgaga tcggggtatt
    26941 gttcaacggg caatgaaaaa atcgccaggg tccctgtcag acacaacagg attgccagca
    27001 cccaggcaaa aatggggcga tcaataaaga aattcgccat taaaagagga cctcgtgttt
    27061 ctgcgtatcg ttgtaggctt gacttgcttc actgtagcgg caaggtacga gccaatcgtg
    27121 gagaaaaaaa ggagttaatg taaattatca tgccctttta tactgaacct taagtgcaag
    27181 tattctttgg tcagtaggag gcattacatc aactgacttt cctcaccctc cgcagagcga
    27241 aagctaatac tcactaacgt gccgccgccg gaaggtggtg aaaaagtcag cgtcccaccc
    27301 agccgttccg cgcgttcgcg catgatattc agaccataat gaccttcggg ttctttcggt
    27361 tcaccgatac caatcccgtt atcacggata taaaccgtgt gattgccgtc cggcgcggtg
    27421 acgcaactga cggcgatttc gctggcgttg gcgtgcttca tcgcattcag caccgcttcg
    27481 cgaataattt gcaacaaatg cacctgcatt tgcgcatcca gcgccagggt tggcagacgg
    27541 cagtcgaggg tcagtttggc gctggtttga ttttgtaacg tatccagcat ttccctcaac
    27601 gcggagggga gatccgcctg ctgcagcgtc aggcggaaag tagtcaacag ctcgcgtaac
    27661 tgccgataag catcattcaa cgcctgggaa aaatcggcca tgatactttg tgcggtggcg
    27721 ttatcttccg gtatcgaacg cttcagtaac gtcaactgga tacgtaagta agaaagtacc
    27781 tgagccagcg agtcgtgcaa ttcgcgggcg atggttgcac gttcttccat caacaataac
    27841 tgctgaaaat gcttttgcgc ctgattaaag tacaaaccgc gtcccagcat cgacgaaacg
    27901 ctgttaagca gcggttctga tgatgaaacg tgactatttt gccagtgcag ttcgccgtaa
    27961 accgtctctt gcattgtcac cggtaaaatc tgcatcggca attccgggtt tggttgccct
    28021 tcgctaatcc gccagttttc accgacattt aactccagat attcagccgc ttcattgtcg
    28081 cgaacaatct gcaaaatatg gcggaaacaa tgcacatcaa tctggcttgt gttcagcgcc
    28141 tgcgaacatt gatacaacac ctccagacga cgcttggcct cgtggagatc gcgggtcttt
    28201 tcttctactg acgcctccag cgaaaggtac aatttatgca gctcgctcga catctggtta
    28261 aaggtttttg caagcagacc aagctcattc ggcagactgg tatccagcgg cggcgagtcg
    28321 aactgcccgt gttcaatacg ttgactggcg gtaaccagct gattcagcgg ggcaaccacc
    28381 tgatggcgta tacggcgcaa agtaaaaaag accagcgtga aaataccgat accgccagcc
    28441 agggaaatcg ccaccaccag cagcattttg cgttcagcgt agtgctgtaa agccagcacg
    28501 aacaggtcta tctgattaac ataattatta atattggcct gataccacgg caaatcgccc
    28561 ttgctgagcc gattattcat ctccagccag ttggcattca gatgcgcata gcgagttttt
    28621 actgcttctg gcacatacca gacgttgaga ttggttaata ccgatgaatg cagtgcctgc
    28681 tgaaacaact ggcgatgtgc attgagttgt ggactgccac tttgcaagtc gtagcccagg
    28741 cggtaactct gcatacgcag cgatccggca atattgatag cctcagcgtc gcgcaaactg
    28801 ctcgccagag ttagcagagc gatacccgtg gaaagaatcg acagcagcac aatgtaaaaa
    28861 aaggcccggg ccagactggc cgagacgggt cgtttaacaa tcacacgcgt cttctccaca
    28921 aaaaatcatt aatgttccag ttcgctaaaa cgcaatttag tcagtgatgg gattatttga
    28981 tctgccacag tttatagcga aatagttact tattctgggc acatgaacat tgccgattcc
    29041 cctgtaagtc gggtaataac aacactcata taaagaataa ggtttttaca accaaaaaag
    29101 aaggtcgtta tgaatcgttt tattatggcc aacagtcagc aatgtctggg ttgtcatgct
    29161 tgtgaaatcg cctgtgtcat ggctcacaat gatgagcaac atgtcctgag ccaacaccat
    29221 tttcatcccc gaattacggt tatcaaacat cagcagcaac gtagtgcagt gacctgtcac
    29281 cattgtgaag atgcgccctg cgcccgtagc tgccctaatg gcgcaatcag ccacgttgat
    29341 gacagcattc aggtcaatca gcaaaagtgt attggctgta aatcctgcgt ggtggcctgt
    29401 ccgtttggta cgatgcaaat cgtcctgaca cccgtcgcgg caggaaaagt aaaagccacg
    29461 gcgcataaat gcgacctttg tgcagggcgc gaaaacggtc ctgcctgtgt cgagaattgc
    29521 ccggcggacg cgctgcaact ggtcactgac gtcgcactct ccggcatggc gaaatcccgc
    29581 cgcttgcgca ccgcacgtca ggagcatcaa ccgtggcatg ccagtaccgc tgcacaagaa
    29641 atgccggtaa tgagtaaagt cgaacaaatg caggcaacgc ccgcgcgtgg cgagccggat
    29701 aaactggcga ttgaagcgcg caaaaccggt tttgatgaaa tttatctgcc attccgtgcc
    29761 gatcaggcac aacgggaagc ctcgcgctgc cttaaatgcg gcgagcacag cgtttgtgaa
    29821 tggacctgcc cgctgcataa ccatattccg cagtggattg aactggtgaa agccggaaac
    29881 atcgacgccg ccgtcgagct ttctcacctg accaacaccc tgccggaaat taccggacgc
    29941 gtttgtccgc aggaccgttt gtgtgaaggt gcctgtacta ttcgcgatga gcacggcgcg
    30001 gtaactatcg gcaacattga acgctacatt tcagatcagg cgttggcgaa aggttggcgt
    30061 cctgacttaa gccatgtcac caaagtggac aagcgggtgg cgattatcgg tgcgggtccg
    30121 gcagggctgg cctgtgcgga tgttctgacc cgcaatggcg tggcggtgac ggtgtacgat
    30181 cgccatccag aaattggtgg cttgctcact ttcggcattc cttctttcaa actggataaa
    30241 tccctgctgg cacgccgtcg ggaaatcttc agcgcgatgg ggattcactt cgaactcaat
    30301 tgtgaagtgg gtaaagatgt ctctttggat tcgcttttgg aacaatacga cgcggtcttc
    30361 gttggcgtag gcacttaccg ttccatgaaa gcgggtttac ccaatgaaga tgcgccgggc
    30421 gtttatgacg cgctgccgtt cctcatcgcc aacactaaac aggtgatggg actcgaagag
    30481 ctaccggaag agccgtttat caataccgcc ggactcaacg tcgtggtact gggcggcggc
    30541 gacaccgcga tggactgtgt gcgtaccgcg ctgcgccacg gcgcgagtaa cgtcacctgc
    30601 gcttatcgtc gtgatgaagc taacatgcca ggctcgaaga aagaggtgaa gaacgcccgc
    30661 gaagaggggg ccaacttcga atttaacgtc cagccggtgg cgcttgagct gaatgagcaa
    30721 ggtcacgttt gcggaattcg tttcctgcgc acgcgtcttg gcgagccgga tgcccagggg
    30781 cgccggcgac ttgtaccgat tgagggcagt gaatttgtca tgccagccga tgcggtgatt
    30841 atggtgtttg gcttcaatcc acacgggatg ccgtggctgg agtcgcacgg tgtaacggta
    30901 gacaaatggg gccgcatcat cgcggatgtg gaaagccagt accgttacca gaccaccaat
    30961 ccgaaaatct tcgccggtgg cgacgccgtg cgtggtgcgg atctggtggt taccgcaatg
    31021 gcagaaggac gtcatgcggc acaggggatt attgactggc tgggggtaaa atcagtcaaa
    31081 tctcactgat agcctgcgca gacaaacccg acttcacagc gtaatataat tgttcatttc
    31141 gcgctgtgga gtcggtatga cgcaacaaat caccctcatt aaagacaaaa ttctctccga
    31201 taactatttc accctgcaca acattactta cgatctcacg cgcaaagatg gcgaagttat
    31261 ccgccataaa cgtgaagttt acgatcgcgg taatggtgcg acgatcctcc tgtacaacgc
    31321 gaagaaaaag accgtggttc tgattcgtca gttccgtgtc gctacctggg ttaatggcaa
    31381 tgaaagcggg cagctgattg aaacctgcgc cgggctgctg gataacgacg aaccggaagt
    31441 gtgcattcgt aaagaagcga ttgaagagac gggctatgaa gtgggcgaag tgcgcaaatt
    31501 atttgaactg tatatgtcgc ctggcggtgt gactgagcta atccactttt ttatcgccga
    31561 atacagtgac aatcagcgcg ctaacgccgg tggcggtgtt gaagatgaag atattgaagt
    31621 gcttgagctg ccgttcagcc aggcgctgga gatgatcaaa accggcgaga tacgtgacgg
    31681 taagacggtg ttattgctta actatttgca aacgtcacat ttaatggact gaaaaataac
    31741 aataatattt cgttgtttat tattggctca atccgacaaa tctgattgag ccgcgctacc
    31801 tgcgcaacga agatacgact tgtgctgttt gtttgaactt ctggggtcgt accgtccatg
    31861 cgctatcgca ttttccttct cttttttttc gctttgttgc cgacgtcttt ggtgtgggcg
    31921 gcactagcgc aacgggcgtt ttccgactgg caggtcacct gtaataacca aaacttctgc
    31981 gtggcgcgca atacgggcga tcataatgga ctggtgatga ccctgagccg cagcgccggg
    32041 gcgcataccg atgccgtttt acgtattgag cgcggcgggt tgaagtcgcc ggatgcgtct
    32101 gaaggagaga tagcgccacg gctgctgtta gatggtgagc cgttggcgtt aagtggtgac
    32161 aagtggcgga tttcaccatg gttattagta accgatgata cggcaaccat caccgcgttt
    32221 ttgcagatga ttcaggaagg gaaggcgatc accttacgcg acggcaatca gaccatttct
    32281 ctgagtggct taaaagcggc gttgttgttt attgatgctc agcaaaagcg cgttggcagt
    32341 gaaaccgcgt ggatcaagaa aggggacgaa ccgccgctca gcgtaccgcc cgcgcctgcg
    32401 ctgaaagagg tcgcggtggt taacccaacg ccgacgccac tctcactcga agaacgcaac
    32461 gatttgctgg attatggaaa ctggcggatg aatggtctgc gctgctcgct tgatccgtta
    32521 cgtcgtgagg tgaatgtcac tgcgctgact gatgataaag cgctgatgat gatcagctgt
    32581 gaggcagggg cctataacac cattgatttg gcatggattg tgtcgcgtaa aaagccgcta
    32641 gcctcgcgcc cggttcggtt gcgtttgccg ttcaacaacg ggcaggagac gaatgaactg
    32701 gaactgatga acgcaacatt tgatgagaaa tcgcgtgaac tggtgacctt agcgaaaggg
    32761 cgcggattaa gcgattgtgg cattcaggcg cgctggcgct ttgacggtca gcgttttcgc
    32821 ctggtgcgtt acgccgcaga acccacctgc gataactggc atgggccaga cgcctggccc
    32881 acgttgtgga tcacccgtta aacaaaatgg cggggacatg taggccggat aagacgctgt
    32941 aggccggata agacgcgtca gcgtcgcatc cggcaatcag catccggcga tcactatcag
    33001 gcacctttca ctcccagcac cttatgcgct tttgccacaa tattctcggc ggtaaagcca
    33061 aagaacggga acagcttatc cgccggagca gattccccat aacccgtcat cccgacaatt
    33121 gcccctttca gaccaacata cttgtaccag taatcggcaa tacctgcttc caccgccacg
    33181 cgagccgcaa cgttagaagg caacaccgac tcccgatatt cctcatcctg ggcgtcgaaa
    33241 atatcggtcg agggcaggga aaccacgcga acattgcgac cttctcctgc taatttctcc
    33301 gcagcttgca gggtgatttc catctctgaa ccggtggcaa tcagaataat atctggctta
    33361 ccgccgctgt ctttcagcac ataaccgcca cgagtaatct ctttaacctg atccggcgta
    33421 cgttccacct gggccagatt ctgccttgag aggatcagtg ccgtcggtcc gttgtggcgc
    33481 tcaaccgcca gcttccagcc caccgccgct tccacctgat cgcacggtcg ccaggtgctg
    33541 aaatttggcg ttaagcgcag gctggccagt tgctcaacag cctggtgcgt cggaccatct
    33601 tcgcccaggc caattgagtc gtgggtataa accataatct gccgcgcttt catcagtgcc
    33661 gccatccgcg cggcgttacg ggcgtattca acaaacatca ggaacgtcgc ggtatacggc
    33721 acaaagccgc cgtggtgcgc gatgccgttg gcgatagcgg tcatgccaaa ttcacgcacc
    33781 ccgtagtgaa tgtagttgcc cgccggatct tccttcagcg aaacagaacc tttccagatg
    33841 gtcaggttgc tgggagccag atccgccgaa ccgccgagca gctccggtag caaggggcca
    33901 taagcgttaa gcgtattttg cgaagcctta cgggtggcga ttttcgccgg attcgcctgc
    33961 aactcattga tatacttctg agtcgttttc tcccagtcct tcggtaaacc accgctcatc
    34021 cgacgggtaa actcttctgc cagttgcgga tgagcctttt tataggcggc aaacttctca
    34081 ttccacctct gctgcgcttt ttcgcctttt tcgcgggcat cccaggcgtg ataaatatct
    34141 ttagggatct caaatggcgg atggtgccag cccagttttt gccgtgccag cgccacttct
    34201 tcttccccca gcggtgcgcc gtgcgcctct tccttacctg ctttattcgg cgatccaaag
    34261 ccaatcaccg tacgacagat aatcagcgac ggcttatctt tcacgctttg cgcttcaagg
    34321 atcgcttcct tcaccgcctg cggatcgtga ccgtcgattt catggatcac atgccagtga
    34381 taggcttcaa aacgttttgc cgtatcgtcg gtaaaccagc cttctgtttc accgtcgatg
    34441 gaaataccgt tgtgatcgta aaaaccaatc agcttgccca gtcccagcgt gcctgccagc
    34501 gaacagactt cgtgggaaat accttccatc aggcagccgt cgcccataaa cacataggtg
    34561 aagtgatcga cgatctcatg gtctggctgg ttaaactgcg ccgccagtgt acgctctgct
    34621 atcgccagcc cgacggcgtt cgccaaacct tgtccaagtg gaccggtggt ggtttcaacg
    34681 cctggcgtat aaccaatctc cgggtggcct ggggttttcg aatgcagttg acgaaagttc
    34741 ttcagctctt ccagcggcag gtcgtaaccg gtcagatgca gcaaactgta gagcagcatc
    34801 gacgcgtgac cgttggaaag aataaagcgg tcgcgatcat accaggttgg gtcggtaggg
    34861 ttatgtttaa gaaaatcgtt ccacagcact tcggcaatat cagccatgcc catcggcgcg
    34921 ccgggatgac cagaattggc tttttgtacc gcatccatac tgagtgcgcg aatcgcattg
    34981 gcaaggtctt ttcgggacat ataacactcc gtggctggtt tatagtttgg cggcaagaag
    35041 atcttccagt ttgcgttgat caacggcgaa cagacgaatg ccttcagaca gtttttctac
    35101 cgccatcgca tactgattgt gctcccaacg gaactccgct tcgctcatgg gagctgggcg
    35161 tgggaacgtc tgagaaggtg ggattaattt acgtaccact ggcgaaactt tttcctgcag
    35221 ctccttcagc aaattcggtg cgatagtcag tcgatcgcag ccggttaagg cgaggatttg
    35281 ttcggtgcga cggaagctcg cgcccatcac aatggtttca tagtggtgtt gcttatagta
    35341 gtcgtagata ttgcgcaccg atttaacgcc cggatcttct tccaccacat acgggtccat
    35401 cggcttgcgt gcctgatacc agtcataaat acgcccgaca aacggcgaaa tcagaaaaac
    35461 gcctgcttcc gcacaggccc gtgcctgtgc aaaagaaaac agcagcgtca ggttgcagtt
    35521 aataccttct ttttccagct cttctgccgc gcgaattcct tcccaggtcg aagccagctt
    35581 gatcagaatg cgtgatttct caacgccttg ttgctgatac aagtccacca gatggcgcgc
    35641 cttctcaata ctcttttctt tatcaaaaga gaggcgtgca tcaacttctg ttgacacgcg
    35701 accgggtacg attttgagga tttcagcacc gaaattgacc gccagtttgt cacacgctgc
    35761 gaccacctgt tgttcctggg tcttgccatt ttttttaccc caggcgatag catcgtctat
    35821 taaatgctca tattgtgata atccggcagc cttgagtaac agcgaaggat tggtggtggc
    35881 atcctgggga tgataatggc gaatggactc aatatcgccg ctgtctgcca cgacagtggt
    35941 gaactgtttg atgccgtcta actcgttcat aggaaatact ccttgaaaag taaagtgtta
    36001 gatgagtgcg ttaattcaca cttctgagaa atttcgctaa acgcatcaaa aaagcatagc
    36061 agacaggcat ggtattgctg gattaagcag gtaacatcag tgttatagga ttattaccaa
    36121 aacattatat taatggtgag agtttggact tgctcaaagt ctgtagactc cggcaaggta
    36181 ataatgtgcg ccacgttgtg ggcagggggt gttgcccaca cactttattt gtgaacgtta
    36241 cgtgaaagga acaaccaaat ggatgaccag ttaaaacaaa gtgcacttga tttccatgaa
    36301 tttccagttc cagggaaaat ccaggtttct ccaaccaagc ctctggcaac gcagcgcgat
    36361 ctggcgctgg cctactcacc cggcgttgcc gcaccttgtc ttgaaatcga aaaagacccg
    36421 ttaaaagcct acaaatatac cgcccgcggt aacctggtgg cggtgatctc taacggtacg
    36481 gcggtgctgg ggttaggcaa cattggcgcg ctggctggca aaccggtgat ggaaggcaag
    36541 ggcgttctgt ttaagaaatt cgccgggatt gatgtatttg acattgaagt tgatgaactc
    36601 gacccggaca aatttattga agttgtcgct gcgctcgaac cgaccttcgg cggaatcaac
    36661 ctcgaagaca ttaaagcgcc agaatgtttc tatattgaac agaaactgcg cgagcggatg
    36721 aatattccgg tattccacga cgatcagcac ggcacggcaa tcatcagcac tgccgccatc
    36781 ctcaacggct tgcgcgtggt ggagaaaaac atctccgacg tgcggatggt ggtttccggc
    36841 gcgggtgccg cagcaatcgc ctgtatgaat ctgctggtag cgctgggtct gcaaaaacat
    36901 aacatcgtgg tttgcgattc aaaaggcgtt atctatcaag gccgtgagcc aaacatggcg
    36961 gaaaccaaag ccgcatatgc ggtggtggat gacggcaaac gtaccctcga tgatgtgatt
    37021 gaaggtgcgg atattttcct gggctgttcc ggcccgaaag tgctgaccca ggaaatggtg
    37081 aagaaaatgg ctcgggcacc aatgattctg gcgctggcga atccggaacc ggaaattctg
    37141 ccgccgctgg cgaaagaagt gcgtccggat gccatcatct gcaccggtcg ttctgactat
    37201 ccaaaccagg tcaacaacgt cctttgcttc ccgttcatct tccgtggcgc gctggacgtt
    37261 ggcgcaaccg ccatcaacga agagatgaaa ctggcggcgg tacgcgcgat tgcagaactg
    37321 gcccatgcgg aacagagcga agtggtggct tcagcgtatg gcgatcagga tctgagcttt
    37381 ggtccggaat acatcattcc aaaaccgttt gatccgcgct tgatcgttaa gatcgctcct
    37441 gcggtcgcga aagcggcgat ggagtcgggc gtggcgacgc gtccgattgc tgatttcgac
    37501 gtctacatcg acaagctgac tgagttcgtt tacaaaacca acctgtttat gaagccgatt
    37561 ttctcccagg ctcgcaaagc gccgaagcgc gtagttctgc cggaagggga agaggcgcgc
    37621 gttctgcatg ccactcagga actggtaacg ctgggactgg cgaaaccgat ccttatcggt
    37681 cgtccgaacg tgatcgaaat gcgcattcag aaactgggct tgcagatcaa agcgggcgtt
    37741 gattttgaga tcgtcaataa cgaatccgat ccgcgcttta aagagtactg gaccgaatac
    37801 ttccagatca tgaagcgtcg cggcgtcact caggaacagg cgcagcgggc gctgatcagt
    37861 aacccgacag tgatcggcgc gatcatggtt cagcgtggcg aagccgatgc aatgatttgc
    37921 ggtacggtgg gtgattatca tgaacatttt agcgtggtga aaaatgtctt tggttatcgc
    37981 gatggcgttc acaccgcagg tgcaatgaac gcgctgctgc tgccgagtgg taacaccttt
    38041 attgccgata cctacgtcaa tgacgaacca gacgcagaag agctggcgga gatcaccttg
    38101 atggcggcag aaactgtccg tcgtttcggt attgaaccgc gcgttgctct gttgtcgcac
    38161 tccaactttg gttcttctga ctgcccgtcg tcgagcaaaa tgcgtcaggc gctggaactg
    38221 gtcagggaac gtgcgccaga actgatgatt gatggtgaaa tgcacggcga tgcggcgctg
    38281 gtggaagcga ttcgcaacga ccgtatgccg gacagccctt tgaaaggttc tgccaatatt
    38341 ctggtgatgc cgaacatgga agctgcccgc attagttaca acttactgcg cgtttccagc
    38401 tcggaaggtg tgactgtcgg tccggtgctg atgggcgtgg cgaaaccggt tcacgtgtta
    38461 acgccgatcg catcggtgcg tcgtatcgtc aacatggtgg cgctggccgt ggttgaagcg
    38521 caaacccaac cgctgtaatt cttttttaac tctcacgctt accctgaata ttcagggtaa
    38581 gcggtttggc tgcaatatat tagtaaagct tattactgag tttgcgaata ataaaaaaag
    38641 cagtctatat aatatctcga tattatttta tttatattca tgcgttgcat atgaaagttt
    38701 atgcaccaca gcgaatatct ctccattctt agtgatctac ctcacctttt aaacgcgctt
    38761 gccgaatttt gttatttact ctgacgaaaa attgtcacga tacacgaaag tttttcacag
    38821 gcggcgactc atggataaag aacgcatcat tcaggaattt gtgcccggca aacaggtcac
    38881 actggcgcat ctcatcgcgc atcccggtga ggaactggcg aagaaaatcg gcgttccgga
    38941 tgcgggcgta attggcatta tgacgctaac tcccggcgaa acggcgatga tcgtcggtga
    39001 tttagcgctg aaagccgctg atgtgcatat cggttttctc gaccgcttca gcggtgcatt
    39061 ggtgatttat ggctcggtgg gcgcagtaga agaagcgtta tcacaaaccg tcagcggttt
    39121 aggtcgctta ttaaattata cgctttgtga aatgaccaaa agttaatacg aggcattcat
    39181 gaaacgaatt gcgtttgtgg gtagcgtcgg ggcaggaaaa acaacgttat ttaatgcgtt
    39241 gcaggggaat tataccctcg ccagaaaaac acaggccgtg gaatttaatg ataagggcga
    39301 tattgatacg ccgggtgaat attttagcca tccccgctgg tatcacgcct taattaccac
    39361 gctacaggat gtagacatgc tgatctatgt ccatggcgca aatgacccgg aaagtcggtt
    39421 gcctgccggg ttgctggata ttggcgtcag caaacggcaa atcgccgtca tcagtaaaac
    39481 ggacatgcca gatgctgacg tcgccgcaac gcgaaagtta ttgcttgaga cgggtttcga
    39541 agagccgatt ttcgagctaa acagccacga cccgcaaagc gtgcagcaac tggtggatta
    39601 tctggcatct ctcaccaaac aggaggaagc aggtgaaaaa actcatcaca gcgaatgata
    39661 ttcgtgaagc acacgcgcgc ggcgaacagg caatgtccgt ggttctgcgc gccagcatta
    39721 ttaccccgga agcccgtaaa gtggcggatc tactgggttt taccatcacc gaatgtgatg
    39781 aatcgatccc ggtaacggcg tccgtgcccg ccagcgtgcc agccgacaaa accgaaagcc
    39841 agcgcatccg cgaaaccatc attgcccagc tgccggaagg ccagtttacc gaaagcctgg
    39901 tcgcacagct gatggaaaaa gtgatgaagg aaaaacagtc gctggagcag ggcgcaatgc
    39961 agccgagctt caaatcggtg accggcaaag gcggcatcaa agtgattgat ggcagcagcg
    40021 tcaagtttgg tcgttttgat ggcgcacaac cgcattgcgt gggcttgact gatcttgtga
    40081 ccggagacga cggcagcagc atggccgccg gttttatgca gtgggagaac gcattcttcc
    40141 cgtggaccct gaactacgac gaaatcgaca tggtgctgga aggggagctc catgttcgcc
    40201 acgaaggcga aaccatgatc gccaaagcag gcgacgtgat gtttatcccg aaaggctcca
    40261 gcatcgaatt tggtacgaca tccagcgtga aattcctgta tgtcgcctgg ccggctaact
    40321 ggcaatccct atgaaagatt tcatcaccga agcatggcta agagcgaacc atacgctcag
    40381 cgaaggcgca gagatccatc tccctgcgga cagtcgcctg acgccctctg cccgggagtt
    40441 gctggaaagc cgccatctgc gcatcaagtt tattgacgag cagggccgcc tgtttgttga
    40501 cgatgaacag cagcagccgc agcccgttca tgggctgacc agtagcgatg aacatccgca
    40561 ggcgtgctgc gaactgtgcc gccagccggt ggcaaaaaag ccggatacgc tgactcacct
    40621 gtcggcggaa aaaatggtcg ccaaaagcga tccgcgtctg ggttttcgcg cggttctcga
    40681 cagcaccatt gcgctggcag tgtggctgca aattgaactg gcggaaccgt ggcagccgtg
    40741 gctggcggat atccgttcgc gtcttggcaa cattatgcgc gccgatgcgc tgggggaacc
    40801 gctgggctgc caggcgattg tcgggctttc tgacgaagat ctgcatcgac tttctcacca
    40861 gccgctgcgc tatctcgacc acgatcatct ggtgccggaa gccagccacg ggcgcgatgc
    40921 ggcgttgctc aatctgctac gtaccaaagt gcgggaaacc gaaaccgtag cggcgcaggt
    40981 gtttatcacc cgcagctttg aagtattacg cccggacatc ttgcaggcgc tgaaccgcct
    41041 ttcgagcacg gtctacgtaa tgatgatcct gagcgtaacg aagccgccgc taacggtgaa
    41101 gcagatccaa caacgactgg gggaaacgca atgattattg aacgttgtcg tgaactggcg
    41161 ttgcgagcgc ccgccagagt ggtttttccg gatgcgttag atcaacgtgt gctgaaagct
    41221 gcgcaatatt tacatcaaca aggtctggca acgcccattc tggtcgctaa tccgtttgaa
    41281 cttcgtcagt ttgcgctcag tcacggcgtg gcgatggacg ggctacaggt gattgatccg
    41341 catggcaacc tcgcaatgcg ggaagaattt gctcatcgct ggctggcccg cgcgggcgaa
    41401 aaaacgccgc cggatgcgct ggaaaaactt accgatccgc tgatgttcgc cgcagcaatg
    41461 gtcagcgccg gtaaagcgga tgtctgtatc gcgggcaacc tctcttccac ggcgaatgtg
    41521 ctgcgtgccg gattacgtat tatcggcttg cagccaggct gtaaaacgct ctcatccatt
    41581 ttcctgatgc tgccacagta cagcggtccg gcgttgggct ttgccgattg cagcgtggtg
    41641 ccacagccga cggcggcgca actggcggat atcgcgcttg ccagtgccga aacctggcgc
    41701 gccctcaccg gagaagagcc gcgcgtggcg atgctgtcgt tttccagtaa cggtagcgcc
    41761 cgtcacccct gcgttgccaa cgtccagcag gcgacagaaa tcgtccgtga gcgcgcacca
    41821 aagctggtgg ttgatggcga gttgcagttt gacgccgcct tcgtgccgga agtggcggcg
    41881 caaaaagcgc ctgccagccc gctacagggc aaagccaatg tgatggtttt tccgtcgctg
    41941 gaagccggaa atattggcta caaaatcgca caacgactcg gcggatatcg tgccgtcggg
    42001 ccattgatac aaggacttgc cgcgccgatg cacgatctct ctcgtggttg tagcgtgcag
    42061 gaaattatcg agctggcgct ggtggcagct gtgccgcgtc agacagaagt gaaccgcgaa
    42121 agcagtttac aaacactggt tgaatgaacg gtcccgttct ggaccctgat aaagaggaaa
    42181 gcacaatgga agcattagga atgatcgaaa cccggggcct ggttgcactg attgaggctt
    42241 ctgacgcgat ggttaaagct gcgcgcgtca agctggttgg tgttaagcag attggcggcg
    42301 gcctgtgtac tgccatggtt cgtggtgatg ttgcagcctg taaagccgcg accgatgcag
    42361 gtgccgcagc ggcacagcgt atcggcgagc tggtatctgt acacgttatc ccgcgtccgc
    42421 acggcgacct ggaagaagtc ttcccgatcg gcctgaaagg cgatagcagc aacctgtaat
    42481 caccctgtgt gacgtcgatt cgggcgtcac gctctctcct ttttcatttt accttctgcg
    42541 gagggtaggg ggagctttta cctgaaatac gccacggagg cgggtatgaa actggcagtc
    42601 gtcactggac aaattgtttg taccgtacgc catcacggac tggcgcatga caaattgctg
    42661 atggtggaaa tgattgatcc acaaggtaat cctgacgggc aatgcgccgt cgccatcgac
    42721 aatattggcg cgggaaccgg ggagtgggtg ttactggtga gtggcagttc cgcccgccag
    42781 gcgcataaaa gcgaaacgtc accggtcgat ctgtgcgtga ttggcattgt cgatgaggtg
    42841 gtgtctggcg gtcaggtaat tttccacaaa taaggcagaa catcatgaat caacaggata
    42901 ttgaacaggt ggtgaaagcg gtactgctga aaatgcaaag cagtgacacg ccgcccgccg
    42961 ccgttcatga gatgggcgtt ttcgcgtccc tggatgacgc cgttgcggca gccaaagtcg
    43021 cccagcaagg gttaaaaagc gtggcaatgc gccagttagc cattgctgcc attcgtgagg
    43081 caggcgaaaa acacgccaga gatttagcgg aacttgccgt cagtgaaacc ggcatgggcc
    43141 gcgttgaaga taaatttgcc aaaaacgtcg ctcaggcgcg cggcacgcca ggcgttgagt
    43201 gtctctctcc gcaggtgctg accggcgaca acggcctgac tttaattgaa aacgcgccct
    43261 ggggcgtggt ggcttcggta actccgtcca ctaacccggc ggcaaccgta attaataacg
    43321 ccatcagcct gattgccgcg ggcaacagcg tcatttttgc cccgcatccg gcggcgaaaa
    43381 aagtctccca gcgggcgatc acgctgctca accaggcgat tgttgccgca ggcgggccgg
    43441 aaaacttact ggttactgtg gcaaatccgg atatcgaaac cgcgcagcgt ttgttcaagt
    43501 ttccgggcat cggccttctg gtggtgaccg gcggcgaagc ggtagtagaa gcggcgcgta
    43561 aacacaccaa taaacgtctg attgccgcag gcgctggcaa cccgccggta gtggtggatg
    43621 aaaccgccga cctcgcccgt gccgctcagt ccatcgtcaa aggcgcttct ttcgataaca
    43681 acatcatttg tgccgacgaa aaggtactga ttgttgttga tagcgtagcc gatgaactga
    43741 tgcgtctgat ggaaggccag cacgcggtga aactgaccgc agaacaggcg cagcagctgc
    43801 aaccggtgtt gctgaaaaat atcgacgagc gcggaaaagg caccgtcagc cgtgactggg
    43861 ttggtcgcga tgcggcgaaa atcgcggcgg caatcggcct gaacgtgccg caagaaacgc
    43921 gtttgctgtt tgtggaaacc actgcagaac atccgtttgc cgtgaccgaa ctgatgatgc
    43981 cggtgctgcc cgtcgtgcgc gtcgccaacg tggcggatgc cattgcgctg gcggtgaaac
    44041 tggaaggcgg ttgccaccac acggcggcaa tgcactcgcg caacatcgaa aacatgaacc
    44101 agatggcgaa tgctattgat accagcattt tcgttaagaa cggaccgcgc attgccgggc
    44161 tggggctggg cggggaaggc tggaccacca tgaccatcac cacgccaacc ggtgaagggg
    44221 taactagcgt gcgtacgttt gtccgtctgc gtcgctgtgt attagtcgat gcgtttcgca
    44281 ttgtttaagg agtaacagat ggcgcacgac gaacaatggc tcaccccacg tctgcaaacg
    44341 gcggcaacgc tgtgtaacca gacgcccgcc gcgacggaat ctccgctgtg gctgggggtg
    44401 gatctgggca cctgcgatgt ggtgtcgatg gttgtcgacc gcgacggtca gtcggtggcg
    44461 gtatgcctcg actgggccga tgtggtccgc gacggcatcg tctgggattt cttcggcgct
    44521 gtcaccattg ttcgtcgcca tctcgacacg ctcgagcagc aattcggtcg ccgttttagc
    44581 catgcggcga cttcatttcc tccaggtacg gacccgcgta tttccatcaa cgtgctggag
    44641 tctgctggac tggaagtgag ccatgtgctg gatgagccaa ccgcagttgc ggatctgctg
    44701 caactggaca acgccggtgt agtggatatc ggcggcggca ctaccggcat tgccatcgtg
    44761 aaaaagggca aggtgacgta ctcggcggat gaagcaaccg gcggacatca catctctctg
    44821 actctcgccg gaaatcgccg tatttcgctg gaagaggcgg agcagtacaa gcgcggtcac
    44881 ggtgaagaga tttggcctac ggtgaaaccg gtgtacgaaa aaatggcgga cattgtcgcc
    44941 cggcatattg aagggcaggg cattactgat ttatggctgg cgggcggctc ttgtatgcaa
    45001 ccgggcgtgg cggaactgtt tcgcaaacaa ttcccggcgt tacaggtgca tttaccgcag
    45061 cacagtttgt ttatgacccc gctggcgatc gccagtagtg ggcgagagaa agcggagggg
    45121 ctatatgcaa aatgaattgc agaccgcgct ctttcaggcg ttcgataccc tgaatctgca
    45181 acgggtaaaa acatttagcg ttccaccggt gacgctttgc ggtccgggct cggtgagcag
    45241 ttgcggacag caagcgcaaa cgcgtgggct gaaacatctg ttcgtgatgg cagacagctt
    45301 tttgcatcag gcagggatga ccgccgggct gacgcgcagc ctggctgtta aaggcatcgc
    45361 catgacgctc tggccatgtc cggtgggcga accgtgcatc accgacgtgt gtgcagccgt
    45421 ggcgcagttg cgtgagtcag gctgtgatgg ggtgatcgca tttggcggcg gctcggtgct
    45481 ggatgcggcg aaagccgtgg cgttgctggt gacgaacccg gatagcacgc tggcagagat
    45541 gtcagaaacc agcgttctgc aaccgcgctt gccgctgatt gccattccaa cgaccgccgg
    45601 aaccggctct gaaaccacca atgtaacggt gattatcgac gcggtgagcg ggcgcaagca
    45661 ggtgttagcc catgcctcgc tgatgccgga tgtggcgatt ctcgacgccg cgttgaccga
    45721 aggtgtgccg tcgcatgtca cggcgatgac cggcattgat gcgttaaccc atgccattga
    45781 agcatacagc gccctgaacg ctacaccgtt taccgacagt ctggcgattg gtgccattgc
    45841 gatgattggc aaatcgctgc cgaaagcggt gggctacggt cacgaccttg ccgcgcgcga
    45901 gagcatgttg ctggcttcat gtatggcggg aatggcgttt tccagtgcgg gtcttgggtt
    45961 gtgccacgcg atggcgcatc agccgggcgc ggcgctgcat attccacacg gtctggcgaa
    46021 cgccatgttg ctgccaacgg tgatggaatt taaccggatg gtttgtcgtg aacgctttag
    46081 tcagattggt cgggcactgc gaactaaaaa atccgacgat cgtgacgcta ttaacgcggt
    46141 aagtgagctg attgcggaag ttgggattgg taaacgactg ggcgatgttg gtgcgacatc
    46201 tgcgcattac ggcgcatggg cgcaggccgc gctggaagat atttgtctgc gcagtaaccc
    46261 gcgtaccgcc agcctggaac agattgtcgg actgtacgcg gcggcgcaat aaatgccgga
    46321 tgcgacgctt gccgcgtctt atccagccta cgggattgca catgtagggc ggataaggcg
    46381 ttcacgccgc atccgccagt ggcgcggtgc aattgccgga tgcgatgctt gccgcgtctt
    46441 atcc
//
LOCUS       DAJVRF010000011        45630 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJVRF010000011 DAJVRF010000000
VERSION     DAJVRF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45630
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.011"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..125)
                     /locus_tag="OQN13_000598"
     CDS             complement(<1..125)
                     /locus_tag="OQN13_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCS1614060.1"
                     /translation="MIIGSIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEG"
     gene            complement(270..863)
                     /locus_tag="OQN13_000599"
     CDS             complement(270..863)
                     /locus_tag="OQN13_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCS1614061.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            934..1647
                     /gene="bdcA"
                     /locus_tag="OQN13_000600"
     CDS             934..1647
                     /gene="bdcA"
                     /locus_tag="OQN13_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1614062.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1778..2173
                     /locus_tag="OQN13_000601"
     CDS             1778..2173
                     /locus_tag="OQN13_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS1614063.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2454..2588
                     /gene="pyrL"
                     /locus_tag="OQN13_000602"
     CDS             2454..2588
                     /gene="pyrL"
                     /locus_tag="OQN13_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCS1614064.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2592..3527
                     /gene="pyrB"
                     /locus_tag="OQN13_000603"
     CDS             2592..3527
                     /gene="pyrB"
                     /locus_tag="OQN13_000603"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCS1614065.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3540..4001
                     /gene="pyrI"
                     /locus_tag="OQN13_000604"
     CDS             3540..4001
                     /gene="pyrI"
                     /locus_tag="OQN13_000604"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCS1614066.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4074..4460
                     /gene="ridA"
                     /locus_tag="OQN13_000605"
     CDS             4074..4460
                     /gene="ridA"
                     /locus_tag="OQN13_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCS1614067.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4666..7362)
                     /gene="mgtA"
                     /locus_tag="OQN13_000606"
     CDS             complement(4666..7362)
                     /gene="mgtA"
                     /locus_tag="OQN13_000606"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCS1614068.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7503..7556)
                     /gene="mgtL"
                     /locus_tag="OQN13_000607"
     CDS             complement(7503..7556)
                     /gene="mgtL"
                     /locus_tag="OQN13_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCS1614069.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7741..8688
                     /gene="treR"
                     /locus_tag="OQN13_000608"
     CDS             7741..8688
                     /gene="treR"
                     /locus_tag="OQN13_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCS1614070.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8807..10228
                     /gene="treB"
                     /locus_tag="OQN13_000609"
     CDS             8807..10228
                     /gene="treB"
                     /locus_tag="OQN13_000609"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCS1614071.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10278..11933
                     /gene="treC"
                     /locus_tag="OQN13_000610"
     CDS             10278..11933
                     /gene="treC"
                     /locus_tag="OQN13_000610"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCS1614072.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12327..14465
                     /gene="nrdD"
                     /locus_tag="OQN13_000611"
     CDS             12327..14465
                     /gene="nrdD"
                     /locus_tag="OQN13_000611"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCS1614073.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14623..15087
                     /gene="nrdG"
                     /locus_tag="OQN13_000612"
     CDS             14623..15087
                     /gene="nrdG"
                     /locus_tag="OQN13_000612"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCS1614074.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15132..15518)
                     /gene="cybC"
                     /locus_tag="OQN13_000613"
     CDS             complement(15132..15518)
                     /gene="cybC"
                     /locus_tag="OQN13_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCS1614075.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15702..17054)
                     /gene="pmbA"
                     /locus_tag="OQN13_000614"
     CDS             complement(15702..17054)
                     /gene="pmbA"
                     /locus_tag="OQN13_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCS1614076.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17148..17699
                     /locus_tag="OQN13_000615"
     CDS             17148..17699
                     /locus_tag="OQN13_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCS1614077.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17782..19155)
                     /gene="mpl"
                     /locus_tag="OQN13_000616"
     CDS             complement(17782..19155)
                     /gene="mpl"
                     /locus_tag="OQN13_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCS1614078.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19331..20329
                     /gene="fbp"
                     /locus_tag="OQN13_000617"
     CDS             19331..20329
                     /gene="fbp"
                     /locus_tag="OQN13_000617"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCS1614079.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20362..21357)
                     /gene="yjfF"
                     /locus_tag="OQN13_000618"
     CDS             complement(20362..21357)
                     /gene="yjfF"
                     /locus_tag="OQN13_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCS1614080.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21344..22366)
                     /locus_tag="OQN13_000619"
     CDS             complement(21344..22366)
                     /locus_tag="OQN13_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1614081.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22380..23882)
                     /locus_tag="OQN13_000620"
     CDS             complement(22380..23882)
                     /locus_tag="OQN13_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS1614082.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24022..24978)
                     /gene="ytfQ"
                     /locus_tag="OQN13_000621"
     CDS             complement(24022..24978)
                     /gene="ytfQ"
                     /locus_tag="OQN13_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCS1614083.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25288..25818
                     /gene="ppa"
                     /locus_tag="OQN13_000622"
     CDS             25288..25818
                     /gene="ppa"
                     /locus_tag="OQN13_000622"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCS1614084.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26197..26538)
                     /locus_tag="OQN13_000623"
     CDS             complement(26197..26538)
                     /locus_tag="OQN13_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCS1614085.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26541..30320)
                     /gene="tamB"
                     /locus_tag="OQN13_000624"
     CDS             complement(26541..30320)
                     /gene="tamB"
                     /locus_tag="OQN13_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCS1614086.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30317..32050)
                     /gene="tamA"
                     /locus_tag="OQN13_000625"
     CDS             complement(30317..32050)
                     /gene="tamA"
                     /locus_tag="OQN13_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCS1614087.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32256..32894
                     /gene="msrA"
                     /locus_tag="OQN13_000626"
     CDS             32256..32894
                     /gene="msrA"
                     /locus_tag="OQN13_000626"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCS1614088.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33217..34560
                     /locus_tag="OQN13_000627"
     CDS             33217..34560
                     /locus_tag="OQN13_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS1614089.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34640..34846)
                     /locus_tag="OQN13_000628"
     CDS             complement(34640..34846)
                     /locus_tag="OQN13_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCS1614090.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35171..35725
                     /locus_tag="OQN13_000629"
     CDS             35171..35725
                     /locus_tag="OQN13_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCS1614091.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35815..36726)
                     /locus_tag="OQN13_000630"
     CDS             complement(35815..36726)
                     /locus_tag="OQN13_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614092.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICKKYKSTIEAFKKEQLALPV"
     gene            complement(36938..37678)
                     /gene="cysQ"
                     /locus_tag="OQN13_000631"
     CDS             complement(36938..37678)
                     /gene="cysQ"
                     /locus_tag="OQN13_000631"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCS1614093.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            37868..39811
                     /gene="cpdB"
                     /locus_tag="OQN13_000632"
     CDS             37868..39811
                     /gene="cpdB"
                     /locus_tag="OQN13_000632"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCS1614094.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(39929..40309)
                     /locus_tag="OQN13_000633"
     CDS             complement(39929..40309)
                     /locus_tag="OQN13_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCS1614095.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40398..41258
                     /gene="qorB"
                     /locus_tag="OQN13_000634"
     CDS             40398..41258
                     /gene="qorB"
                     /locus_tag="OQN13_000634"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCS1614096.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            41366..42331
                     /locus_tag="OQN13_000635"
     CDS             41366..42331
                     /locus_tag="OQN13_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1614097.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            42439..43101
                     /gene="ytfE"
                     /locus_tag="OQN13_000636"
     CDS             42439..43101
                     /gene="ytfE"
                     /locus_tag="OQN13_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCS1614098.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43146..44558)
                     /gene="cycA"
                     /locus_tag="OQN13_000637"
                     /gene_synonym="dagA"
     CDS             complement(43146..44558)
                     /gene="cycA"
                     /locus_tag="OQN13_000637"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCS1614099.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(44867..45487)
                     /gene="fklB"
                     /locus_tag="OQN13_000638"
     CDS             complement(44867..45487)
                     /gene="fklB"
                     /locus_tag="OQN13_000638"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1614100.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
BASE COUNT        11166 a        12153 c        11369 g        10942 t
ORIGIN      
        1 ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc tttgatatgc
       61 tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat acttccgatg
      121 atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat acgaaaagat
      181 gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta tcggcaggcg
      241 cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt tatcgcttcc
      301 cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg agccttcgca
      361 gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct ttgtggatgc
      421 cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg aacggcaata
      481 tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc agcgcagccg
      541 ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca ctcccctacc
      601 ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac atattcattg
      661 agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct cggggggtta
      721 ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt ttggtgaaaa
      781 agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg acctggaacg
      841 cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa acagttttgt
      901 aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg taagacagtt
      961 ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt cgtcaccgat
     1021 ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca cctggcacaa
     1081 aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt cattgatgtc
     1141 gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg cgtctttggc
     1201 gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa tattcatgct
     1261 ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg catcttaatc
     1321 atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta tgccgccagt
     1381 aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc gcgtgggatc
     1441 accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc caacgggcca
     1501 atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc ggaagaggtc
     1561 gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg cgcgatgcat
     1621 accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca gccatttccc
     1681 atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg gtaaatataa
     1741 atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa ccgctgtttt
     1801 ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta ttcgttccgg
     1861 cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac cagaacccga
     1921 ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg ctgctgcggg
     1981 atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag aaaaccaatt
     2041 tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc caaactggac
     2101 ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga tagcgcgcat
     2161 ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct ataagtcgga
     2221 tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa gcaggaaatg
     2281 tctgcaatta ttgataccga acgaccgttc ccctgcagaa tcacaacaaa taaaaatgca
     2341 tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg catcaaatgc
     2401 ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga tgtatggttc
     2461 agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg ccgtttttct
     2521 tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc ccaggcgtca
     2581 ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata aacgacctta
     2641 gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca aacccgcaac
     2701 cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct acccgtaccc
     2761 gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc ttctccgaca
     2821 gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt tcggttatca
     2881 gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg cgcctggcca
     2941 ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac caacatccga
     3001 cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg gacaatctcc
     3061 acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg actcaggcgt
     3121 tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg gcaatgccgc
     3181 aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac agctctattg
     3241 aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa gagcgtctgg
     3301 acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc gatctccaca
     3361 acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag attgcgacgg
     3421 atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg attttcgctc
     3481 gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg gaaatagaga
     3541 tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta attgaccata
     3601 tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa acggatcagc
     3661 gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat ctgatcaaaa
     3721 tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat gcgccgcaag
     3781 ccacggttaa ccgtatcgac aactatgaag tggtgggtaa atcacgcccc agtctgccgg
     3841 agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat gccgaaccgg
     3901 tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa tgcaaatact
     3961 gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg gtaataaaag
     4021 tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa atcatgagca
     4081 aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag ggcgttgatc
     4141 tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg ggcgaagtac
     4201 cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg atcgtcgaag
     4261 ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa gatctgaacg
     4321 acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat gccaccttcc
     4381 cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt gagatcgaag
     4441 cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg gattaagtct
     4501 gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg attcctgcaa
     4561 tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga acaaaactca
     4621 ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt gccagccgta
     4681 acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg ccagaatcgc
     4741 aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac tggccagcgg
     4801 tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca ttaacggcca
     4861 cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca caatcagcgt
     4921 ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg tttccggcgt
     4981 gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga tcgagctgat
     5041 cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct gcggcttttg
     5101 aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat catacagcag
     5161 gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag cactcgccac
     5221 cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt atttcagcat
     5281 gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca ggcttttttc
     5341 cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca cagaaatgcc
     5401 gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa agccaaccac
     5461 atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg tcagacgagc
     5521 aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg tttcaatatc
     5581 actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt tcgctgcaac
     5641 taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta atgccttcag
     5701 tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt cgaggatcag
     5761 gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt tcgtcgccac
     5821 cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa tcttacgcag
     5881 catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac acacattgag
     5941 gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt ctgccactac
     6001 caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt gccaacggct
     6061 ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat cgagtaggtt
     6121 tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc gttcgctggt
     6181 tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca gggtgccagt
     6241 tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca gatgtttgac
     6301 gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg taacaatcat
     6361 cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg ccgcttccca
     6421 ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca ccagcataaa
     6481 gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg gctcgctttc
     6541 ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg tagcaatcac
     6601 cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg tgtcacactc
     6661 cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg gcagagactc
     6721 accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac gtaaatctgc
     6781 cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct ggtcgatcgg
     6841 gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg tcgcggtatt
     6901 gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct gaataaagtt
     6961 cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata aatcttcagt
     7021 ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc gatagcagac
     7081 ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt caccatgttg
     7141 ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt cgaacgtttt
     7201 ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta aggacggcgg
     7261 gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa ccagacggga
     7321 aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc cctccgcact
     7381 gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa ggtaataaag
     7441 ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt gaaaagacgg
     7501 gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg ttccatataa
     7561 cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat attcgtagca
     7621 tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac tttacccatt
     7681 gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca ggacaacagg
     7741 atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg gaaatctaca
     7801 gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga gcgtgttgaa
     7861 gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat gcgtgggcaa
     7921 agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga aaatctcgcc
     7981 gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat gatggaaagc
     8041 cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg taatatcgac
     8101 ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca ctggcagtca
     8161 tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta tgacgacgaa
     8221 ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa tatcagttat
     8281 ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc ctacctggcg
     8341 ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc tatgaagcaa
     8401 ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact gtgcgcaacc
     8461 gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga caccttgcaa
     8521 ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat cgtcaccgta
     8581 gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc acaggtaacc
     8641 gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc gtttcctgaa
     8701 cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat ttttccgcgc
     8761 aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga tgagcaaaat
     8821 aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca atattgcgac
     8881 ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca atgccagacc
     8941 gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg ggcaatttca
     9001 ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt caaccggaca
     9061 ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga aatggcatga
     9121 gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt tgattagcgg
     9181 cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca acggtcagac
     9241 gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt tgatcggtga
     9301 agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa aaatgggcgg
     9361 cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc tgatgaacgc
     9421 ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca gcatcgccaa
     9481 agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc tgggcgttat
     9541 tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg tgcccgtctg
     9601 ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg gtcgcatgat
     9661 tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg ctccgattgg
     9721 tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac accagaccac
     9781 ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt ggccgctgat
     9841 tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt ccagccgcaa
     9901 gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg gggtcactga
     9961 gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg cgatgattgg
    10021 ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg gtatcggcgt
    10081 agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat ttgcgctggc
    10141 aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc agcggaaata
    10201 ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg ccccctcgca
    10261 tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc gttatctacc
    10321 agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat ttacgtggcg
    10381 ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg ctaaccccct
    10441 tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg gcgattgatc
    10501 ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa tcgcgcggga
    10561 ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc tggtttcgcg
    10621 aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat ggcgaaccag
    10681 aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc tggcatgcgg
    10741 aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc aactgggaga
    10801 atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat cgtggggtcg
    10861 acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc cctgaagacc
    10921 tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag tttttgcacg
    10981 agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa atgtcctcca
    11041 ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg tcgatgacct
    11101 ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg ctggctaaac
    11161 ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg cacaacgtgg
    11221 catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct cgctttggtg
    11281 atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgctg catggcatgc
    11341 agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg catttcacgc
    11401 gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg cgcaacgatg
    11461 gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac aacagccgca
    11521 cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa ccgtggattg
    11581 gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat gactcctcgg
    11641 tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc ctgacatggg
    11701 gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc cgtgaatgga
    11761 aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc tggcagccag
    11821 ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc tcaccacaac
    11881 cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag taaatcttcc
    11941 ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt gtttttgttg
    12001 atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc cgtcatccgt
    12061 acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt gatgcaaagc
    12121 actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc gactataatt
    12181 gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg gcgattgcgg
    12241 aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt ctgtggataa
    12301 cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga gacggctgca
    12361 aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct aaagcagcgg
    12421 aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag cagatgcagg
    12481 gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag ctgatgtcgg
    12541 gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc gacattgaac
    12601 gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag accaacgcct
    12661 cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag cgcgacctgc
    12721 tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt gacgtggtgc
    12781 aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg ttcttcccga
    12841 tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt aaaatgggga
    12901 acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact gcgcagatta
    12961 ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc gatgaagtgc
    13021 tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa gagtggaaca
    13081 tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac gatgccttcc
    13141 agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg ccgtttgtaa
    13201 cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa tcgatcctgc
    13261 gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg aaactggtgt
    13321 ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac atcaaacagc
    13381 tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac gatcaggtag
    13441 tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc ggcgtgtggg
    13501 aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg atcagcctga
    13561 acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg aagctgctgg
    13621 atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt ctcgaaggcg
    13681 tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta cgtctgaatg
    13741 ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg ctgggttaca
    13801 tcggcatcca cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac gacaacgagc
    13861 agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg gatcagtgga
    13921 aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac ctgtgcgacc
    13981 gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc gataaaggtt
    14041 actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac gacaagatcg
    14101 actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac ggcgagtatc
    14161 caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc tatcagcatg
    14221 taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc tttaccggtg
    14281 agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat gacgcctccc
    14341 gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg cgtccgttta
    14401 acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat gggcagatag
    14461 gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct tatccggcct
    14521 acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag gcattgatta
    14581 ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta tcatcagtac
    14641 tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt ctccgggtgt
    14701 gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc tggtcagcca
    14761 tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg tatcaaacgc
    14821 caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc ggatattctg
    14881 aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt gtggacaggc
    14941 tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat taacgtgctg
    15001 gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg cggcagcagc
    15061 aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc aggcaacagg
    15121 gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt cagttgctct
    15181 gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc gtcgtcaatc
    15241 tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct gtccggtgat
    15301 ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc gcgcatcttc
    15361 gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt taaattgtcg
    15421 ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact gaataccaac
    15481 gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct tttttattta
    15541 cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg tcacgtacta
    15601 cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa tgattttgtg
    15661 taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc ggcgattttc
    15721 atctccggca acagcacaga accacactgt atattactgc gtgtttcaat atcgttaccg
    15781 acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat ttcgctcacc
    15841 ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga ataatcaccg
    15901 gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt gcccatctct
    15961 ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg aataccgccc
    16021 gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag cagccactga
    16081 gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc gctgtcgaat
    16141 ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag ccagtccggc
    16201 agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac cgatccaccc
    16261 gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc aaaaatgact
    16321 ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg gcgagcacag
    16381 tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat ggtgtaggcg
    16441 taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag cgaatgacgc
    16501 gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac accgtagtgg
    16561 ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa tgctgcctgt
    16621 tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg gtggaacaag
    16681 tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc acagggatct
    16741 ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat ggcctgcggg
    16801 cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt gatccccagc
    16861 gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct tacgctaatg
    16921 ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc caactccagc
    16981 gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga gattactttc
    17041 attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc gtttttcagt
    17101 gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg actaagcagc
    17161 ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac gatgaaatta
    17221 tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc cttggcgcgg
    17281 aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg gatctgcgcg
    17341 cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag ttgcagctca
    17401 tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg gataagctga
    17461 aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg cgcgatcgtc
    17521 tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc gatgccgatc
    17581 gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg aataagccgc
    17641 cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat gaagggtaat
    17701 tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta tccggcaatg
    17761 ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca gaccatccag
    17821 cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat ggtcgccagg
    17881 ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc agtgtgcagg
    17941 ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg gttgcagcag
    18001 gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga tccccatttt
    18061 catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac caactttgcc
    18121 acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat cgtcatatac
    18181 cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa taaacgaacc
    18241 cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg caatcgccat
    18301 caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc ccactttttc
    18361 gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg cctgccagtg
    18421 accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg tctgtttcag
    18481 gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac gcaccagatg
    18541 gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat cgaactcaag
    18601 gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt tgtcgaagaa
    18661 ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca gacgcgccga
    18721 aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac cacactgttc
    18781 cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg caacggccag
    18841 cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca tataagggat
    18901 gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt tgccaataat
    18961 caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca gctcaatgcc
    19021 ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac ccgttacttc
    19081 atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac aaattcctaa
    19141 aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt acgcatatgt
    19201 cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga ttcacctgag
    19261 cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata aacattgcag
    19321 ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg agttttctca
    19381 tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca agattatcca
    19441 tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg ctgagaacgc
    19501 gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga aagccgcact
    19561 gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga ttgtcgtctt
    19621 tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg gctcgtccaa
    19681 catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg ttacgcctgt
    19741 tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg ttgcggcagg
    19801 ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg gtgttcacgc
    19861 ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga tgcgcttccc
    19921 ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc cgaacggggt
    19981 gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc cgtatacctc
    20041 gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag gcggtattta
    20101 tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt atgagtgcaa
    20161 cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca aagagcgtat
    20221 tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg gcaacgacca
    20281 tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat ctcctgcgcc
    20341 tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca ctggcgaact
    20401 ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa taaacaacaa
    20461 aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt ttatgtaagt
    20521 ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc caacgccacc
    20581 gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca cacctacgcc
    20641 cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca gcgttgccag
    20701 tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc ccatcagatt
    20761 cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg tacgatgcgc
    20821 gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac ttaagcgacc
    20881 gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat ggtttatcgg
    20941 aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc ccgcaagcgt
    21001 aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg cgccgaaggc
    21061 acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga aatcgccaat
    21121 cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga gatcgatccc
    21181 accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg cgttatcggt
    21241 caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca ggcagtagag
    21301 atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt ttatcacggc
    21361 tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc acacaaagca
    21421 ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc aaaattccgg
    21481 tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta aagcgaccgc
    21541 ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc agccataatc
    21601 cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc gcaatcgccg
    21661 cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg actccggcat
    21721 tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc gccgttttgc
    21781 gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc gttggcagga
    21841 acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg acgatctgcc
    21901 cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg gtggcgacaa
    21961 acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct aggatgccgg
    22021 tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc atcgcagccg
    22081 ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca ccggtggcga
    22141 tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta agaatgtcga
    22201 tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc ggggctacca
    22261 ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc gtcggccagt
    22321 gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca ctgttctcct
    22381 tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg gatctccgcc
    22441 acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag ctcttccagt
    22501 tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat caggcggatg
    22561 atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat cagaaattgc
    22621 ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc ggagagaaat
    22681 tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa gcgttcggca
    22741 atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc ctggagagcg
    22801 aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt cctgtcttcc
    22861 gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt ttgcggtttg
    22921 cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac ttcggcggtt
    22981 tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg gcgcacttcg
    23041 agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc aacgggtttg
    23101 tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc gcgccccagc
    23161 atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc tacgaaactg
    23221 ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag aaagtgagtg
    23281 acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa taatagctcc
    23341 acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt ggcggagaga
    23401 tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg gttaagcggt
    23461 tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg cttttccatc
    23521 tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag attatcagca
    23581 accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc gagttgttgt
    23641 gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt gccgcgatcg
    23701 gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc gttttcacca
    23761 agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa cgctttgacg
    23821 ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg gtcggtcgtc
    23881 atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg gtaagcgtcg
    23941 catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg gcctacgaat
    24001 cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta actcttcttt
    24061 tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag gcatggtgcc
    24121 gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg gcgtcagttc
    24181 aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta caccgtcaat
    24241 ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg cctgaattgc
    24301 accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc cgttgttttc
    24361 cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt cacctgactg
    24421 cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc ctttcttacg
    24481 gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc atggtttgcc
    24541 attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt tgtcggcagt
    24601 aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga gcaagaagac
    24661 cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg caaccactgg
    24721 agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt taatctggtt
    24781 ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt cggcctcact
    24841 tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct gcgaaaatcc
    24901 tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg cagagactac
    24961 aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg tgaaaagatg
    25021 ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc acacttcaaa
    25081 aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta tgaaaaatgc
    25141 ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga aaaaggaaaa
    25201 tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc ggcacttgtt
    25261 tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc gggtaaagat
    25321 ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc gatcaaatac
    25381 gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac cgcgatgttc
    25441 tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga cccggttgac
    25501 gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg ccgtccggtt
    25561 ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc ggttccgcac
    25621 agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc tgaactgctg
    25681 aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg caagtgggtg
    25741 aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc ttccttcgag
    25801 cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg cttcggttag
    25861 taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt attctcagcg
    25921 gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct gttcatagcc
    25981 acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc ccccgtttta
    26041 cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata tggaaagcgg
    26101 ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga tacatcgcat
    26161 aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt ctaaccagtc
    26221 gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca tccatgcact
    26281 cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc gcaaggcatc
    26341 aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg ttccgttccc
    26401 cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga tactgaaatc
    26461 gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc gtaaactgcc
    26521 gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag tgcctggtct
    26581 acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa cgtgagtgtt
    26641 gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa tacatagccg
    26701 ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa attgcttacg
    26761 ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc cagaccaatc
    26821 agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg tccacgtagc
    26881 aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc cgctttcggt
    26941 tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt agcatccggg
    27001 ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa cagtaactca
    27061 cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg gatgttgatc
    27121 tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc cgtcagccgc
    27181 gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat caggttactg
    27241 ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt aagcatcacc
    27301 acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat gcgcgcccac
    27361 gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa tacaacatct
    27421 ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact gcctttcgcc
    27481 gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt caggtagatt
    27541 tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt aaagttgacc
    27601 gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc cacacccgta
    27661 acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg caagttggca
    27721 ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat cgcaaggttg
    27781 aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc ggtcacctgc
    27841 acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa ttcggcacgg
    27901 ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag cgccgcatcg
    27961 ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc ctgcggcagc
    28021 ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc gctggcctga
    28081 gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag attcaccacc
    28141 gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc attcgggtta
    28201 agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc cagcgcaata
    28261 tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct aagagttccc
    28321 ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc ggagacaggc
    28381 tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc attcagggtg
    28441 acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc gagtttcccg
    28501 gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac ggaaagttcc
    28561 tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc caccgtgccg
    28621 cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt atccagcccc
    28681 ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc tttcacttcg
    28741 gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat ataactgttg
    28801 cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt cagcttcagt
    28861 tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt aatcaagcca
    28921 ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt aagcgttagc
    28981 tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc agttttccct
    29041 tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc tttggcatca
    29101 agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat agagagcgtg
    29161 taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata ctgcttctca
    29221 ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg caatccggct
    29281 tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag gttaacgcct
    29341 atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc acctttcaac
    29401 ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc tgacagctgc
    29461 gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc gtccagttta
    29521 gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat gtccgtgtcg
    29581 cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag cggcaaatgc
    29641 acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt ttcgccgagc
    29701 ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc ctgcgccact
    29761 tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt cagggtcagg
    29821 gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt ggtgtcatca
    29881 atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata cggcgtggag
    29941 agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg cattttttta
    30001 ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac gctactgttc
    30061 cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc cacgcctggc
    30121 tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac gttgccaata
    30181 tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc gctggtggtg
    30241 cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac gccgaggctg
    30301 atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt aacccgtgtt
    30361 cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc ggcgattccc
    30421 agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg cttaccgctt
    30481 cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac tggtattcca
    30541 gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat ttcggggcga
    30601 tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa cgcagatccg
    30661 gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt gtcacaaaac
    30721 gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg gagaaatcaa
    30781 catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag tcgccccagg
    30841 ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga taaaacagca
    30901 tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc aggttaatgg
    30961 cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta gtggagtcag
    31021 attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa tattgttcca
    31081 gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc ggcgcggaaa
    31141 tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt ttccacgtcg
    31201 ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg gtgttttctg
    31261 ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct ttatcaaatt
    31321 gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg ttcagttccg
    31381 ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt tgcaggtatt
    31441 catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt tcgccactgt
    31501 tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc tgcgctttgg
    31561 taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac tttttgaaat
    31621 tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg agcaatttca
    31681 aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg ccaattaaca
    31741 ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt ggcggtggac
    31801 ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct tcacggatgg
    31861 catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt tcaatcgtag
    31921 aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc tgtagacgga
    31981 cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat aactgtcgga
    32041 tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc cgttaaacgg
    32101 cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat atttcttatg
    32161 ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt gttacaacct
    32221 taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat aaaaagcatc
    32281 tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta gccacgctgc
    32341 atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt gccatttttg
    32401 cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc gtttacagca
    32461 ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa gtgtgctccg
    32521 gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc atcagctatg
    32581 agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg cgtcagggca
    32641 atgaccacgg cacgcagtat cgttcagcga tttatccgct gacccctgaa caggatgccg
    32701 cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat gacgatcgtc
    32761 acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat gaccaccagc
    32821 aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc gtctgtctgc
    32881 cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc tctttgtcta
    32941 tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt caatatattg
    33001 caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt tatcagcagt
    33061 ctgaatttta accctctggc gactttacag taccttacgc tatactagcc actgaaaatg
    33121 tcggttcact ttcttcgaat cggctttcaa tgtgtatttc acacaaatta atcaactttc
    33181 ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat tttagtcata
    33241 ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct tgccgcctca
    33301 cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca acgcgttctg
    33361 aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct gaacgcagtg
    33421 gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca cagcctgttc
    33481 tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc tttctcgtta
    33541 gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg tatgattgcg
    33601 ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta cgtttgcacc
    33661 ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt caaactgcca
    33721 atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga agccggtgcg
    33781 ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt tgagctggaa
    33841 tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt tgatctccac
    33901 gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa gttcctcgtc
    33961 tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct gctgaaccgc
    34021 gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa cacgctgatt
    34081 gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc aggtgaagac
    34141 ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct caacgacgtg
    34201 atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat tgtcgcccgt
    34261 gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat gcgcgtgctg
    34321 gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt tatgatgttt
    34381 atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta caaatttgaa
    34441 gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat cgacagcaag
    34501 gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag cgtcgcgtaa
    34561 ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc atagcacttt
    34621 ttactgcata gcactttggt taagccatct ctgtttgcag acgcataacc tgacggttaa
    34681 cttcggacat cactgacaaa tgcagcttat ccttcacttt tgggataaga atcttaccct
    34741 tatcaaattc aaaagcgcca acgtccttga tgtataaccg tccacggaac aggatcttta
    34801 cgtacttcgc cacctgaagt gggttgtacc gttggaaaat tttcattgtt atctcctgct
    34861 gagtattacg cctgtgcggt gccacaatcg gcccaactat tatgaggcgc aaattttaat
    34921 gcttagtgac tatagactat ccgggcaatg tttccaccgc gtataacttt ttttacctaa
    34981 aggttacaat tattcagaat tatcttttta ccgaagcgcg gtcttcagta taagcattca
    35041 tttttcatat gatttgtgcg cttgaccgca aactggcatc acacttgcgg gaaattcgat
    35101 aaatagcaca tatgattaaa actcagaccc aagtggtcgg atcacctgca tatcataaga
    35161 aggaaacacc atgaccctac gcaagattct ggcactcacc tgcctgctgt tgccgatgat
    35221 ggcttccgca catcagttcg aaaccggtca gcgagtgccg ccgattggca ttaccgatcg
    35281 gggcgagttg gtgcttgata aagatcagtt tagctacaaa acctggaaca gcgcgcagtt
    35341 agtgggaaaa gtgcgagtac tgcaacatat tgctggtcgc acctctgcaa aagagaaaaa
    35401 cgcgacgctg attgaagcga ttaaatcagc gaagttaccg cacgatcgtt accagaccac
    35461 cactattgtt aacaccgacg acgcgattcc gggttcaggg atgtttgtgc gcagcagtct
    35521 ggagagtaat aaaaagcttt atccctggtc gcagtttatt gtcgatagca atggcgtcgc
    35581 gcgcggtgcc tggcagctgg atgaagaaag ttccgctgta gtggtgctgg ataaggatgg
    35641 tcgcgtgcaa tgggccaaag acggggcgct cactcaggaa gaggtgcagc aagtgatgga
    35701 cctgctgcaa aagttgctca aataatggca acaacataga ttctgcaaaa ataattttcc
    35761 cctatcgtta tctatgggat aggggaatta agtctctgag ttcttacgcc gaactcatac
    35821 tggtaacgct agttgctctt ttttaaacgc ctcaatggtc gacttatatt tcttgcaaat
    35881 cttttctttg gtaatattac tatctgcggc cagattcgca cttactatcg catccacttg
    35941 ttcggctaat tccacttgtt ggcaacctgc attaaccaga aaattataaa taagggagta
    36001 atcttcatcc tccaaaacaa cagaggaacc attacgaaaa cgttcatccc ctccatagat
    36061 tttaagcgca aagcccaaag attctatgac tatggcaaaa ttatcatcaa ttattttttc
    36121 gtgaatatac catccatcgc attctttaat tccacctcct ggtatttgga gttcaaattt
    36181 accccgtttt aatttgctta ctatatcctc caataaggca taacctaaag acacgtcacc
    36241 aatcgcctgg tgaatataac ctttaatcct tttcggtatg tcagactgtt ttttctgcag
    36301 aatatcacag gcatacaatc ttctgttttg ttctaacgga aactcgcggt ttttgaaatc
    36361 catgaatgag ttatactcat ttattgttat gggaatttct tcgtattcaa atccactacc
    36421 tcgcaatgtc attgtatatt cggcaccatt atctttaagg ctgaaactga taaggaactt
    36481 atccatggcg ttaacagttc cattttttga tgaaagtacc tgataactat ttttatcaat
    36541 ctgagttgcg caacgaaaga actccctcaa agttaacagg ctatcataat tcgaaatcca
    36601 atcttttttc ttaaatgcat gctggatatt gttaaaaaaa ttgtttgaat gtgtttttgt
    36661 atacgatgac tttacaaata actccaccgc accaataaaa ttattgtgtc tgatcctggg
    36721 taacatatga caaccttatt ttatttggtt attttatgtc gccattaaat atctcattag
    36781 agaagtaaac tatcaaaaaa cgctcattat taaaatgtaa cttattagca attgaatttt
    36841 tgcactttaa atacaatctc tttttattta taacttttta ggttgggaga aatcaggatg
    36901 gcagaatcag gaaatacact gtttctgcca tctgaattta gtaaatagac actctgaacc
    36961 ccggattcag gaacgactcg cgcggagtgt aatccagcgg tttaccctgc cagtcgtgaa
    37021 cgtgcgctcc ggcagctgca gccacagcat gtccagcggc ggtgtcccaa atattcgttg
    37081 gtccgaagcg cgggtacagc tgcgcctgtc cttccgccac caggcagaat ttcagcgaag
    37141 agccgatgga cgtggtctga tgttcgccaa gctgttgcag atactctttc agctccgcat
    37201 ctgcatggga acggctgata accaccagcg gcgggcgcgc atcgcggacc tgaatctgct
    37261 tgcgcacacc gcactcttct ttccaggcct tgccttctgc cgcgctgtac attacgttca
    37321 ttaccggcgc atacaccaca cctaaaatcg gtttgccatg gtcaatgagc gcaatgttaa
    37381 cggtgaattc gccattacgt ttaataaact ctttagtacc atccagcggg tctaccagcc
    37441 agtaacgctg ccagtgctga cggacttccc aaccgggagg atcttcttca gaaaggaccg
    37501 gaatatccgg tgtcagcgta cgtaaaccgt ccatgataac ggtgtgtgcg gcaatatccg
    37561 ctgccgttac cggagaattg tccgctttgc tgacgacgtc catcggtttc gtcccgtcgt
    37621 agacctgcat aatggcatcg cctgcattcc gtgcaagctg gcatacttga tctaacattt
    37681 ctccacctcg tctctgtgag cggtgttaac ttattgtttt acttataccc tatcgttaat
    37741 gaatgcgcca actgtgatag tgtcatcatt ttcaaagcgt aaaattgtgg cattcttcac
    37801 tgttctataa gtaagacgtt tattctccct tttctttcgt attcccgatg ataaaaggat
    37861 gtccctgatg attaagttta gcgcaacgct cctggccacg ctgattgccg ccagtgtgaa
    37921 tgcagcgacg gtcgatctgc gtatcatgga aaccactgat ctgcatagca acatgatgga
    37981 tttcgattat tacaaagaca ccgccacgga aaaattcgga ctggtacgta cggcaagcct
    38041 gattaacgat gcccgcaatg aagtgaaaaa cagcgtactg gtcgataacg gcgatttgat
    38101 tcaggggagt ccgctggcag attacatgtc ggcgaaagga ttaaaaacag gtgatgttca
    38161 tccggtttat aaggcgctga atacgctgga ttatacggtc ggtacactcg gcaatcatga
    38221 atttaactac ggtctggatt acctgaaaaa tgcgctggcg ggagcgaaat tcccttatgt
    38281 aaatgccaac gtcattgacg ccagaaccaa acagccaatg tttacaccgt atttaattaa
    38341 agataccgaa gtggtcgata aagacggaaa aaaacagacg ctgaagattg gctatattgg
    38401 cgtcgtgccg ccgcaaatca tgggctggga taaagctaat ttatccggga aagtgacggt
    38461 gaatgatatt accgaaaccg tgcgcaaata cgtgcctgaa atgcgcgaga aaggtgccga
    38521 tgtcgttgtc gttctggcac attccgggct gtctgccgat ccgtataaag tgatggcgga
    38581 aaactcagtt tattacctca gtgaaattcc gggcgttaac gccattatgt ttggtcatgc
    38641 tcacgccgtt ttcccgggta aagattttgc tgatatcgaa ggggctgata tcaccaaagg
    38701 cacgctgaat ggtgttccgg cggtaatgcc gggcatgtgg ggcgatcatc ttggggtggt
    38761 cgacttacaa ctcagtaatg acagcggtaa atggcaggtg acgcaggcga aagcggaagc
    38821 tcggccgatt tacgacatcg ccaataaaaa atccctcgcg gcggaagaca gcaagctggt
    38881 agaaacactc aaagccgatc acgatgccac acgccagttc gtcagcaagc caatcggtaa
    38941 atctgccgac aatatgtata gctatctggc gctggtgcag gacgatccga ccgtgcaagt
    39001 cgtgaacaac gcgcaaaaag cgtatgtcga acattacatt cagggcgatc cggatctggc
    39061 aaaactgccg gtgctttcag ctgccgcacc gtttaaagtt ggtggtcgca aaaatgaccc
    39121 ggcaagctat gtggaggtgg aaaaaggcca gttgaccttc cgtaatgccg ccgatcttta
    39181 tctctacccc aatacgctga ttgtagtgaa agccagcggt aaagaggtga aagagtggct
    39241 ggagtgctcc gccggacagt ttaaccagat tgatcctaac agcacgaaac cacagtcact
    39301 catcaactgg gatggtttcc gcacttataa ctttgatgtt attgatggtg tgaattatca
    39361 gattgatgtt acccagcccg cccgttatga cggcgagtgc cagatgatta ataccaatgc
    39421 ggaaaggatt aagaacctga cctttaatgg caagccgatt gatccgaacg ccatgttcct
    39481 cgttgccacc aataactatc gcgcttacgg cggcaaattt gccggtacgg gcgacagcca
    39541 tatcgctttt gcttcaccgg atgagaaccg ctcggtgctg gcagcgtgga ttgctgatga
    39601 gtcgaaacgt gcgggggaaa ttcacccggc ggcagataac aactggcgtt tagcaccgat
    39661 agctgccgat aagaaactgg atatccgttt cgaaacttcc ccgtcagata aagccgcagc
    39721 gtttattaaa gagaaagggc agtatccgat gaataaagtc gcgaccgatg atatcgggtt
    39781 tgcgatttat caggtggatt tgagtaagta aaacacttct ttttcggcct ataaatcatc
    39841 aatcgcatcc ggcatttatt ggcggatgcg atgctggcgc atcttatccg ccctacaagt
    39901 catacaccgt aggccgcgca tccggcagtt atgccgcacg ttcatcccgc accgccaaca
    39961 cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg ctcacctgct
    40021 cgcccagcgg cgtgaggctg tattcaacat gcggtggcac caccggatac gcgatacggt
    40081 taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt tcgctcaccc
    40141 cgccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc gccaccagaa
    40201 tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg cactgttccg
    40261 caaagagatt accctctttc agttgttgcg acaggctaac ctgactcatt tcatacttac
    40321 ctttttgtac gtacttacta aaagtaagtt taggtgttag cgtatttaaa cacaagacaa
    40381 aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt ggtcactata
    40441 ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc gttcgtaatc
    40501 cggcaaaagc ccaggctcta gcagcacaag gcattaccgt gcgtcaggct gactacggcg
    40561 atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg atctcttcca
    40621 gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca aaggcggctg
    40681 gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg ctcggcctcg
    40741 ccgatgagca cattgagacg gagaaaatgc tggctgattc tggcatcgtt tacaccctgc
    40801 tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca ctggaacacg
    40861 gcgtatttat cggtgcggcg ggcgatggca aaatcgcctc agcaacgcgg gcagattatg
    40921 cggcagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt tacgaactgg
    40981 cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa cagagcggca
    41041 aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg aaaagcgtcg
    41101 gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca tcgaaaggtg
    41161 gtctgtttga taacagcaaa acgcttagca aattgattgg ccgcccaacg acaacgttag
    41221 ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg gcatcctccc
    41281 gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc tgaactttct
    41341 tctgacagac caatggatgc cagtaatgat tagcggcgtg ctgtacgccc tgttagcagg
    41401 gttgatgtgg gggcttattt ttgtcgggcc gttgatcgta ccggaatacc cggcgatgtt
    41461 gcagtcgatg gggcgttatc tggcgttagg gttaattgcg ctgcccattg cctggctggg
    41521 acgcgtgcgt ctgcgtcagt tggcgcgtcg cgactggctt accgccttga tgctcaccat
    41581 gatgggcaac cttatctatt acttctgcct tgccagtgcc attcaacgta ctggcgcgcc
    41641 tgtttccacg atgattatcg gcaccctgcc ggtggtgatt cccgtttttg ccaatctgct
    41701 ttatagccag cgcgacggca aactcgcatg gggaaaactc gccccggcac tggtttgtat
    41761 tggcatcggc ctggcgtgtg tgaatattgc tgagttaaac cacggactcc ccgattttga
    41821 ctgggcacgt tatacctctg gcatcgtgct ggcgttagtt tccgtggtct gctgggcgtg
    41881 gtatgccctg cgcaacgccc gctggctgcg ggaaaatccc gacaaacatc cgatgatgtg
    41941 ggcaacggcg caggcgctgg tcacactgcc ggtttctctc atcggctatc tcgtcgcctg
    42001 ttactggctg aatatacaaa cgccggactt ctccttacct tttggccccc gtccgctggt
    42061 gtttattagt ctgatggttg cgatagccgt gctttgctca tgggttggcg cactctgctg
    42121 gaacgtcgcc agccagcgat taccgacagt gattctcggg ccgctgattg ttttcgaaac
    42181 gctggcaggt ttgctgtaca cctttttact ccgccagcaa atgccgccgc taatgacgct
    42241 gagcggtatc gcgctgttag tgattggcgt ggtcattgcg gtcagagcaa aaccagaaaa
    42301 acctttaact gaatctgtct cagaaagttg acacgctggc agtgagttaa ataagcctct
    42361 gctacgtaag ggttatagct tttgccttaa agatgcattt aaaatacatc ttatattatt
    42421 aagaatgagg tatcagctat ggcttatcgc gaccaacctt taggtgaact ggcgctctct
    42481 attcctcgcg cttcagctct gtttcgtaaa tatgatatgg attactgctg tggcggtaag
    42541 caaacgctgg cgcgcgcggc ggcacgtaaa gaactggatg ttgaggtcat tgaagctgaa
    42601 ctggcaaagc ttgctgaaca accgattgag aaagactggc gtagcgcccc gctggcagaa
    42661 atcattgacc atatcatcgt gcgctaccac gatcgtcacc gcgagcaact gccggagctg
    42721 atcctgcaag cgactaaagt cgagcgcgtt cacgccgaca aaccgagcgt gccaaaaggg
    42781 ctgacaaaat acctgactat gctgcatgaa gagctatcca gccacatgat gaaagaagag
    42841 cagatcctct tcccgatgat caaacaaggc atgggcagcc aggcaatggg accaatcagc
    42901 gtgatggaaa gcgagcacga tgaagcgggc gaactgctgg aagtgattaa acacaccacc
    42961 aataacgtca caccgccgcc ggaagcctgc accacctgga aagcgatgta taacggtatt
    43021 aatgaactga ttgatgacct gatggatcac atcagcctgg aaaacaatgt actgttccca
    43081 cgcgcgctgg cgggtgagta atctaaaagc tggatggcat tgcgccatcc agcatgataa
    43141 tgcggttatt tccgcagttc agcagcccgc ttcttaccaa taaacaacca gcccagcccc
    43201 agtgcgataa accacagcgg ggtgaccaac agcgcctggc gagtgtcatc ttccagtgtc
    43261 agcaacacga ccacgaacac aaagaacgcc atacataccc agcacatcag cttgccgagc
    43321 ggcatcttgt agatcgactt ctcatgcaga tgaggacgct gtttgcggta aaccaggtac
    43381 gagcaaagga taattgtcca gacgaacata aacagaatcg cggaaacggt tgtaatcatc
    43441 gtgaacgcgc caatcacgct aggattcaca tacaacatca ccacgccgcc gagcagacag
    43501 atacacgaga aggtcaaccc tttcgcgggt actgcgcgct tagaaagttt agcgaacgct
    43561 ttcggtgcca caccttcctg cgccagacca aacagcatac ggctggtaga gaagacgccg
    43621 ctgttagcgg aagacgccgc agaggtcagc accacaaagt tgatcacgct ggcagccgca
    43681 ggcaaaccta ccaacacaaa cagttcaaca aacgggctct tctccgggac taccgaactc
    43741 cacggcgtca cggacataat cacaatcagc gcgaagacgt agaacataat gatacggatc
    43801 ggaatggagt taatcgcgcg tggcagtgat ttctccggat ctttggtttc cgcagcggtt
    43861 gtacctacca gctcaatccc tacgaaagcg aaaaccgcta tctggaatcc ggcaaagaag
    43921 ccacttaaac ctttcgggaa ccagccgcca tcattccaca aatgcgcgaa tgacgcttcc
    43981 acaccagtcg gtgactgaaa gtgcatcgcc accataacca ggccgacgac aatcagcgac
    44041 acaatggcga cgattttgat catcgcaaac cagaactcca tctcaccgaa cattttcacg
    44101 gtggcgagat tgagcgtcag cagcagcact atcaccgcca gcgaggcgac ccagtcggag
    44161 agatcgggga accagaactg agcataagca gtgatcgcca ccacgtctgc cataccggtt
    44221 acaacccagc agaaccagta agtccagccg gtgaaatatc ctgcccacgg cccgagtaaa
    44281 tcggaagcga agtcactaaa agatttgtat tccagattcg aaagcagcaa ttcccccatt
    44341 gcccgcatca cgaaaaagag cataaaaccg atgatcatat aaacgaaaat gatcgacggc
    44401 ccggcaaggc taatcgtttt gccggacccc ataaacaacc ccgtaccaat ggcaccgcca
    44461 atggcaataa gctgaatatg tcggtttgtg agattgcgcc gtagcgactg ttcagccgga
    44521 gcctgatcat cggcaacgac ttttacctga tctaccatgt ttttttcttc ctgtacctgt
    44581 ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat ctgtatcggg
    44641 actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat ctaatgttaa
    44701 ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc acaaaaatac
    44761 acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa gggtttattg
    44821 ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag aggatttcca
    44881 gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcgcct gcgccgcgct
    44941 cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc ggcatcagag
    45001 tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg ggttcaccac
    45061 gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa tgaacacgaa
    45121 cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg aattgcaggc
    45181 cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat ttcacacctt
    45241 cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg tggatttcac
    45301 gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc agcgcatcgg
    45361 caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca ctcagttgtt
    45421 gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca aaagttgggg
    45481 tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc agccacactt
    45541 ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag aaacaatagc
    45601 aatatcccgt atgaatataa taaaggcggg
//
LOCUS       DAJVRF010000012        45278 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJVRF010000012 DAJVRF010000000
VERSION     DAJVRF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45278
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.012"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(85..744)
                     /gene="pgmB"
                     /locus_tag="OQN13_000639"
     CDS             complement(85..744)
                     /gene="pgmB"
                     /locus_tag="OQN13_000639"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCS1614101.1"
                     /translation="MKLQEVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(741..3008)
                     /gene="ycjT"
                     /locus_tag="OQN13_000640"
     CDS             complement(741..3008)
                     /gene="ycjT"
                     /locus_tag="OQN13_000640"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCS1614102.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEVHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3005..4060)
                     /locus_tag="OQN13_000641"
     CDS             complement(3005..4060)
                     /locus_tag="OQN13_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1614103.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4070..4858)
                     /locus_tag="OQN13_000642"
     CDS             complement(4070..4858)
                     /locus_tag="OQN13_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCS1614104.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(4876..5928)
                     /locus_tag="OQN13_000643"
     CDS             complement(4876..5928)
                     /locus_tag="OQN13_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCS1614105.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(5959..6562)
                     /locus_tag="OQN13_000644"
                     /pseudo
     CDS             complement(5959..6562)
                     /locus_tag="OQN13_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(6549..7430)
                     /locus_tag="OQN13_000645"
     CDS             complement(6549..7430)
                     /locus_tag="OQN13_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCS1614106.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(7451..8761)
                     /locus_tag="OQN13_000646"
     CDS             complement(7451..8761)
                     /locus_tag="OQN13_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1614107.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(8796..10306)
                     /locus_tag="OQN13_000647"
                     /pseudo
     CDS             complement(8796..10306)
                     /locus_tag="OQN13_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
     gene            complement(10518..10832)
                     /gene="pspE"
                     /locus_tag="OQN13_000648"
     CDS             complement(10518..10832)
                     /gene="pspE"
                     /locus_tag="OQN13_000648"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCS1614108.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(10907..11128)
                     /gene="pspD"
                     /locus_tag="OQN13_000649"
     CDS             complement(10907..11128)
                     /gene="pspD"
                     /locus_tag="OQN13_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCS1614109.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(11137..11496)
                     /gene="pspC"
                     /locus_tag="OQN13_000650"
     CDS             complement(11137..11496)
                     /gene="pspC"
                     /locus_tag="OQN13_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCS1614110.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMLDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(11496..11720)
                     /gene="pspB"
                     /locus_tag="OQN13_000651"
     CDS             complement(11496..11720)
                     /gene="pspB"
                     /locus_tag="OQN13_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCS1614111.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(11774..12442)
                     /gene="pspA"
                     /locus_tag="OQN13_000652"
     CDS             complement(11774..12442)
                     /gene="pspA"
                     /locus_tag="OQN13_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCS1614112.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVNDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            12609..13586
                     /gene="pspF"
                     /locus_tag="OQN13_000653"
     CDS             12609..13586
                     /gene="pspF"
                     /locus_tag="OQN13_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCS1614113.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(13704..14969)
                     /gene="puuE"
                     /locus_tag="OQN13_000654"
                     /pseudo
     CDS             complement(13704..14969)
                     /gene="puuE"
                     /locus_tag="OQN13_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15007..16287)
                     /gene="puuB"
                     /locus_tag="OQN13_000655"
     CDS             complement(15007..16287)
                     /gene="puuB"
                     /locus_tag="OQN13_000655"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCS1614114.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(16289..17779)
                     /gene="puuC"
                     /locus_tag="OQN13_000656"
                     /pseudo
     CDS             complement(16289..17779)
                     /gene="puuC"
                     /locus_tag="OQN13_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(17929..18486)
                     /gene="puuR"
                     /locus_tag="OQN13_000657"
     CDS             complement(17929..18486)
                     /gene="puuR"
                     /locus_tag="OQN13_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCS1614115.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(18513..19277)
                     /gene="puuD"
                     /locus_tag="OQN13_000658"
     CDS             complement(18513..19277)
                     /gene="puuD"
                     /locus_tag="OQN13_000658"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCS1614116.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            19489..20850
                     /gene="puuA"
                     /locus_tag="OQN13_000659"
     CDS             19489..20850
                     /gene="puuA"
                     /locus_tag="OQN13_000659"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCS1614117.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            20999..21163
                     /locus_tag="OQN13_000660"
     CDS             20999..21163
                     /locus_tag="OQN13_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614118.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            21153..22538
                     /gene="puuP"
                     /locus_tag="OQN13_000661"
     CDS             21153..22538
                     /gene="puuP"
                     /locus_tag="OQN13_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCS1614119.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            22672..22917
                     /locus_tag="OQN13_000662"
     CDS             22672..22917
                     /locus_tag="OQN13_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCS1614120.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            23230..24873
                     /gene="sapA"
                     /locus_tag="OQN13_000663"
     CDS             23230..24873
                     /gene="sapA"
                     /locus_tag="OQN13_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCS1614121.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            24870..25835
                     /gene="sapB"
                     /locus_tag="OQN13_000664"
     CDS             24870..25835
                     /gene="sapB"
                     /locus_tag="OQN13_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCS1614122.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            25822..26712
                     /gene="sapC"
                     /locus_tag="OQN13_000665"
     CDS             25822..26712
                     /gene="sapC"
                     /locus_tag="OQN13_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCS1614123.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            26712..27704
                     /gene="sapD"
                     /locus_tag="OQN13_000666"
     CDS             26712..27704
                     /gene="sapD"
                     /locus_tag="OQN13_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCS1614124.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            27706..28512
                     /gene="sapF"
                     /locus_tag="OQN13_000667"
     CDS             27706..28512
                     /gene="sapF"
                     /locus_tag="OQN13_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCS1614125.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            28580..28933
                     /locus_tag="OQN13_000668"
     CDS             28580..28933
                     /locus_tag="OQN13_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCS1614126.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            29301..30089
                     /gene="fabI"
                     /locus_tag="OQN13_000669"
     CDS             29301..30089
                     /gene="fabI"
                     /locus_tag="OQN13_000669"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCS1614127.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            30234..31360
                     /locus_tag="OQN13_000670"
                     /pseudo
     CDS             30234..31360
                     /locus_tag="OQN13_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            31428..33362
                     /gene="rnb"
                     /locus_tag="OQN13_000671"
     CDS             31428..33362
                     /gene="rnb"
                     /locus_tag="OQN13_000671"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCS1614128.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAKKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(33440..33543)
                     /locus_tag="OQN13_000672"
                     /pseudo
     CDS             complement(33440..33543)
                     /locus_tag="OQN13_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            33597..35587
                     /gene="pdeR"
                     /locus_tag="OQN13_000673"
                     /pseudo
     CDS             33597..35587
                     /gene="pdeR"
                     /locus_tag="OQN13_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            35734..35907
                     /locus_tag="OQN13_000674"
     CDS             35734..35907
                     /locus_tag="OQN13_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614129.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            35997..36746
                     /locus_tag="OQN13_000675"
     CDS             35997..36746
                     /locus_tag="OQN13_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1614130.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37015..37233
                     /gene="osmB"
                     /locus_tag="OQN13_000676"
     CDS             37015..37233
                     /gene="osmB"
                     /locus_tag="OQN13_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCS1614131.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(37359..37685)
                     /gene="yciH"
                     /locus_tag="OQN13_000677"
     CDS             complement(37359..37685)
                     /gene="yciH"
                     /locus_tag="OQN13_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCS1614132.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(37685..38422)
                     /gene="pyrF"
                     /locus_tag="OQN13_000678"
     CDS             complement(37685..38422)
                     /gene="pyrF"
                     /locus_tag="OQN13_000678"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCS1614133.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(38615..39784)
                     /gene="lapB"
                     /locus_tag="OQN13_000679"
     CDS             complement(38615..39784)
                     /gene="lapB"
                     /locus_tag="OQN13_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCS1614134.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(39791..40099)
                     /gene="lapA"
                     /locus_tag="OQN13_000680"
     CDS             complement(39791..40099)
                     /gene="lapA"
                     /locus_tag="OQN13_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCS1614135.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(40248..41012)
                     /gene="pgpB"
                     /locus_tag="OQN13_000681"
     CDS             complement(40248..41012)
                     /gene="pgpB"
                     /locus_tag="OQN13_000681"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCS1614136.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFCIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            41182..41772
                     /gene="ribA"
                     /locus_tag="OQN13_000682"
     CDS             41182..41772
                     /gene="ribA"
                     /locus_tag="OQN13_000682"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCS1614137.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(41836..44511)
                     /gene="acnA"
                     /locus_tag="OQN13_000683"
     CDS             complement(41836..44511)
                     /gene="acnA"
                     /locus_tag="OQN13_000683"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCS1614138.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(44675..44770)
                     /locus_tag="OQN13_000684"
     CDS             complement(44675..44770)
                     /locus_tag="OQN13_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614139.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(44884..45051)
                     /locus_tag="OQN13_000685"
     CDS             complement(44884..45051)
                     /locus_tag="OQN13_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614140.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(45054..45218)
                     /locus_tag="OQN13_000686"
     CDS             complement(45054..45218)
                     /locus_tag="OQN13_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCS1614141.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
BASE COUNT        10709 a        11943 c        11407 g        11219 t
ORIGIN      
        1 ttgaagtcct tcaccacatg cacctggtta tcgtagattt tttgaatatg ttgtaacgaa
       61 agctgagcca tgttgattcc tttgctatac gttttgccag aaggccgata accgcgacca
      121 ggtcagggag tccgttgaag gcaatagtaa ttgcgccccg gttatgcccg cgccgatccc
      181 caccgagcgc ataccgctgg cgttaatggc gtcaatgccc gcctgcgcat cttcaatgcc
      241 gatacatgcc tgcggcggca cgcccagccc ggcacaggca gcgagaaaga tttccggatc
      301 cggtttcgag tttttaagtt gggaagcatc cgcgcagaag gtgaaaaact cacgcagctc
      361 cagtgcatct aaaatcgtcg gcgcattgag ggagacagaa gccagcccta ccgagatctg
      421 ttgtgcgcgg agatctgcca gcaaagagcg aatgccgggt agaacagcgt tgaccgtcaa
      481 ctcgcgcaat gagtggacat agagcagatt tttgcgatac gccagttgcg ccctctcctg
      541 cgagttaaag tcgccctctt tgcccccgtg ttgcagaatg cgccgcagag actcatcgcg
      601 gctgatccct tttagggatt cgttaaactg cgcatcaatg ctgatgccaa tttcagcggc
      661 aatctgctgc cacgcctgga aatgcagatg cgcggtatcg gtgattacac catccagatc
      721 gaaaattacc tcttgcagtt tcattcatcc tcctgatgtg tggcagcggt cccattgaag
      781 ggcaaaataa aatcacccaa acagaaaaca gattcttcag ccacggatat aagctgaccg
      841 ttcaaacgca gtgaaacggg ggcagaagtt cgaatcgcaa tacgctgcgc gtcaagagtg
      901 acctgtaatt cgcagccctg ccagaacaga gggaaagaca actgttgcca ctgctcaggt
      961 aacgccggat tgagatgcaa ttcaccgtca cgcacgctca ccccggcaaa accctgaatc
     1021 gcccccagcc agatagcgcc agttgcggca gcatggatac catcatcaca actatgcgga
     1081 tcagcaccaa gatcgatttc agtcccctcg cgccagaact gataactttg ggtcagcagg
     1141 ccacagcgtg cggcaacaat gccgtggatt gctttactta atgacgagtc gtgaatagtg
     1201 cgcggttcat aaaattgcag attggcaaga cacgatgccg ctgagaactg ctccggcagc
     1261 atgtaattga gcatcaccac atcagcttgt ttgaggattt gcatctcgtt cacttctgcg
     1321 cgtgaataat ccagcagaat ggtttgcttc cccgccgccg ctttgtattt cgcaagatta
     1381 atcgccggct tagccataaa cgaatcatcc tgcggcaaaa cgccgtcggg ctgaatttct
     1441 ggcagccgca gttctttaag gaacatttcg gcacgatgaa taaacgcgtc gtcgctacag
     1501 ccaaactggc gggcaatact cagcgcctgc tggacgttgt aatacgccat atagctggtg
     1561 aaggcgttat tattgacatg ttcggtatat tcgtcaggcc caataacatc atgaatttcc
     1621 agacggtcgt taaccctcac cgcgcggcta atccagaact ttgcagtttc cagaagtagc
     1681 gccatgcctt catgcgcaat gaaactttca tcccccgtgg tctgccagta ttgaataacc
     1741 gcccaggcga tatcggccac cagatgatgt tccgcctgcg ccgaggccac tttttgccgc
     1801 agcccggtgc gaatgttaat ggcggcaaat tccggcgtct cttcttcgcc gctgcgcgcg
     1861 ctttcccacg gaaatagcgc gccctgccag ccgttgcgtc gcgctttctc ctgcgcgcct
     1921 ggcaagttgt gccagcgata acgcagtaaa cttcgggcaa ccgtcggatc gctaaacaga
     1981 tgaaacggta acaaaaatac ttctgtatcc cagaaaacgt ggcctttgta gccttcgccg
     2041 gttaagcctt ttgccgcaat gctgctgcgc tcgtcgtggg caggcgtcat gatgcgcaga
     2101 tgataaagcg cataatctaa cgcttgctga tcgtgcactt cgccgccatt taccgtgata
     2161 cgacgtttct gccaccattg acgccagttt tctgttgata ctgcaagaag ttggtcgtaa
     2221 ctctgctgcg cgcacatttc aagctggcga agcgacgcgc tgccccactc gtctaaaacg
     2281 gcttgcctgt catcccgcca gtcgatccac accagttttt gcaacgtcac tgtctcgcct
     2341 gcatgaagct gcgcactggt atgttgcagt aaacggcgct ctttggcggt atagcattgc
     2401 tgcacatcac cgctcacctt acagcaacag ctgatggcca catcactgcg tccatcctgg
     2461 gtggtgtaga tcccctgcat caaatgctga ccaaacaccc gcacctgggt ttcgtcgaga
     2521 tgctggcgac cgtggttggt ctgcgtggcg tcaatgcctg ttgaaatcag cactgacgcg
     2581 tcggcgtcca gtggcgtaat agtgatttcc agcacaatga gcggcagttg gtctgccgaa
     2641 acaaagcgac ggctggcgat ggtgtaacct gtgccgttgc tggtacgcca gacaacgcta
     2701 cggcgtaatt ctccactggc aaagtcaagt tcacgctgcc aggcttcgcg ggataacgag
     2761 aaaacctcac catttatggc aatctccatc cccacgacat caggcaggtt caccagttcg
     2821 ttgatttcac cttttcccgc ccgatgatac agccccgcca gatacatccc tcgcgtctgg
     2881 cgcgtgtaat cttcttcatg gctggcgcga agcccaagat agccgttccc ctgcgccatc
     2941 agcgatgcat acttgttcag ggtatgctgg ctgaaatggg gttctgataa cgttactggc
     3001 ctggtcataa ttccacacgc gtccctgttt ctgcggattg atacaacgcc gccaccagtt
     3061 gctggatgat gtacccctgc ttggcgtcgg ctatcatcac gggcttgccc tgtacgtgat
     3121 tgataaaggc ttccatgctg cgcaaatggc ggttgtcgtc tgccattttc cgttgcatca
     3181 gcgtcattaa ttcaccgttg ttatcggtgt agatatgtgc tggaaacagc gtcgcaccag
     3241 ctttatcacc acaaaagctg acgttcataa tcgactgttc gcggatgttg agtgcaaatg
     3301 acgtttccag ccacagaatg ccgccgttat gaaattcaat ggtgccaaac agcgaatctt
     3361 cgacgctgta agttgccgga tcccactcac caaattgacc acagctcttt tgcgtgccga
     3421 tcttttgaaa gctatgcgca ttcacgcttt tcaccgccgg aaagcccagc acatacatcg
     3481 cagcatccag catatgaatg ccgatgtcga tcagcgggcc accaccctgc agttctttat
     3541 tggtaaagac accccagccg ggaacgccgc agcgacgcag ggcgcgggcg gtggtaacgt
     3601 aaatctctcc caaaacgccg ttggtcacct gttcacgcag ctgttgcgta tcgagcgcaa
     3661 aacgatggtg aaagtcgtag gccagcacct tgcccagttt gcgcgcggta tcgcacattt
     3721 cccgcgcctg ttctggcgtc atggcgagcg gtttttcgca catcacatgg cagcccgctt
     3781 ccagtgccat cagggtatgt tcgtaatgaa aacggttagg tgagcagacg ctaaccacat
     3841 caggtttcac cgccagcagc atggcctgcg gatcgtccca cacggatgca ttcccgtatt
     3901 tttctgccag cgcctgcgcc tgggaaaggc ggctgtcaca gacagccacc agttccagat
     3961 cgttgcgggt gcagtagtac gaagcatgaa ccttatccgc cacctggcct gcgcctatta
     4021 tcgcgacccg cagcggagag cttgtcattg cacttttcac aaagacctct tagcaggtac
     4081 gcaaccaggc caacgaatca cggtacgcct gggcaggatc ttccgcccgg atacgccctt
     4141 cgtacactac atagccctga tagttatccg cgcgcagctg ttcaaacagc gcgtggaaat
     4201 ccagggtgcc gctgcccggc tggtagcgat ggttatccgc aatatgcaca tgaccgagca
     4261 ggtcgcggtt gtcatgcagc gcctgcgcca ggttatcttc ttcgatattc atgtgataga
     4321 aatcgccgat aatctgtaca tgtttaagat cgttttcgac gatgtaacgg cgggcatcgg
     4381 cgagggtgtt gatcatatga tcctgatagc ggtttaacgg ttcgagatac accactgttc
     4441 cggtacgcgc ggcgacctgt tccagtacgc gcagggaatc actcaccatt ttgcggtcgc
     4501 cgtccaggct acgcggcgag gtcatcggcg gtaagcggaa ggtaaacatg ccccacgctg
     4561 ccggaacgac gataccttta tcccccactt ctgccagcgc ttcgagaatg cgttcgatct
     4621 gctttaagcc attaagacga cgctcttcga taaagtcgcc aatccaaccg tcatagccac
     4681 cgcaggcggt ggtcaccggt aaaccggttt ctttgattgc tgctttgact tcttcgagat
     4741 tgttgaccag cagtttgccg tcaatctcaa aaccatcgaa gcccatctct ttgatataac
     4801 gaaatttctc cagaatgttt tccggaaaga atgcctgatt ctgtgtgcct attttcattt
     4861 tttcattcct ttgaattaaa acgtaacgcc cattttgatg ctctgttccg gatgctgatc
     4921 gacatactgc atgtagcttt ccgggctatt ggcaaaggtc actaccgggt cgattaaatc
     4981 ttcgcaattg agataaccgt tcatcagcag ttcccagcag gtttcttcaa tacgcttacg
     5041 gctccagcgc ggataatccg ggttcggttc gctgcacgcg cgggagaaaa caattttggc
     5101 gttattgaaa tgcgcttcgc gtccgaggtt aaaaccttca gcaaatggtt tggcaaacgc
     5161 aacataggag atggtgccac cgtaggccag accgcggaac gccgattgca gcgcgtcggc
     5221 atagccgctg gtttcgataa tcacatcggc accctgcttg ccggtcagcg ttttgatctc
     5281 tttgcctacg tcagtaccaa tggggttaag gcagaaatcc gcgccgtggc gacgggcaat
     5341 atcacagcga tgagcgatag gatcgacgcc aatcacgacc gacgcgccag cacgtttagc
     5401 cagttgaatg gcaatttgac caatcgcgcc aagccctacc accacaacaa aatcacccac
     5461 gcgcacgttg gcatcgcgca cgccgctcat ggcaaactgc gccgggtcgt aacaaacggc
     5521 gtttttccac gagctgcctt ccggcatttt gcgcagcttg tagttattca ccgcgttaat
     5581 gatgaccgtc tcggagagcg ggccgtagcc gcatacgctg tcgcccaccg cgtagtcggt
     5641 aacgtcgctg ccgcactcga taatgtcgcc aaccaccatg tttccaagct ggaatttgcc
     5701 aaactcaatg ccgcgcggcg catctgccgg acgcggagtg aacatctgcc attcgccgtt
     5761 aaagtcttca tcgataaacg ggctggcggc gcggaagtcg accacttccg ttccgtgttt
     5821 cggtgcgccg aaacgcacgc ggaccttcac ttcattagct aaaatcgccc gatcttcata
     5881 ctcaaccagc gcagcaacac gcggtgctgt ggctactaac tttttcattt aaaactcctt
     5941 gtaaagaaac tggccgcttt agcccttcac gccaccggcg gtcaaaccac tcttaataaa
     6001 acgttccgaa agcgcataca taatcaccac cggcaatgcg gtcaccagtg aggccgccat
     6061 catccgtccc cagatgtaat ctggcgtact gaacagcgcg ttcaggccca ccggtaaggt
     6121 gaaattgccg gcgctggaga ggaaaatcga cgcaaacaga tagtcgttcc acgccaccat
     6181 aaagcagtag acgaacacgg atatcagccc ggacatcgcc aacggcacgg tgatgcggaa
     6241 gataatttgc agacggttga ggccatccat catcgccgct tcttcgatct catcggggat
     6301 ggtgtcgaag tagcttttca gcataaacac ggcggtgggc agagtttgcg tcaccatggt
     6361 gatgatcagc gccatctcag tcgacgtaat gctccagcgt ccactgctgc ggcagcagcg
     6421 taggatgcag tgagatcgcc tctttcgcgc ccttgaacga ggtcatcagc atcacaaaaa
     6481 atggaaacag cgtgatgatg agaaacagcg ccagcccgca gtaaaaaccg atgcgactga
     6541 gtgtgcgttt atttgttgcc attgaggttc acccgtttcc tggtcagcag aatgacagcg
     6601 aaaataatga tgaagagcac caccgaaatc gccgccgctt tccctaagtc attaaaggca
     6661 aaggcggttt tgtagagata gacgccgaga atatcgacct tggtggtcag cagataaaca
     6721 tcagcaaaca tgtagaacat ccagatagtg cgcagggtca ccaccgtcgc caggaccggc
     6781 ataattgcgg gcagcgtgac gatgcgaaac cgctgccagg cattagcgcc atccatctct
     6841 gccgcttcgt acagcgattt gtcgatggtc tgcaaaatcg ccagaaacga gataaaggca
     6901 tacgggaagt agcgccagat ggcgaacagc accaccagca caaagctact gcccggatta
     6961 tcgaaccaca gcggtgcctg ctcatagaga tgcagaagat cgacgccgag gtagttcaca
     7021 atgccgtagc cgttgttgaa catgtatttc caggcaaaca ccagcgaaat ggacggcgtt
     7081 acgtaggaga ggatcaccag cgaacgcgcg gttttgcgca ggcggaactc gcggttgaaa
     7141 aacatcgcca ctgccagccc gagcacggtg ctgcccgcca ccaccagcgc ggtataccag
     7201 accgtcatcc acagcgagtg ccagaagccg ggatcggaga ggatacgcac atagttactc
     7261 accccaacaa aggttgactc gatgttggga ttgagcggca gacgtaaaaa actgatttcg
     7321 atattcgaca ccattggcca ggccaccaga ccacccagca gtaataagct gggcgcgaga
     7381 agcagcagcg caaagggcat atcggaacga cctgaaaaaa gcctgttcat tgcggattcc
     7441 tgtcctgggt ttagtgctgt tcgatcagtt catccagctt tttctggctc gcttgcagcg
     7501 tgccagggag atcggcttta cccacggtga cgttatgcac catggagctc accacgccag
     7561 aacccgtcac atcacccatg cgggtaaagt ttttatcccc tactgcgcca aagacctgaa
     7621 tatttggcag ttcaccgatt agctgattcg gtagttcacc cagcgcctta ataacgtcgt
     7681 tgtctttcca ggtggcggta gtgacaaccg ctttattcac cggcagcgca gcacctggcg
     7741 acatcatcac ccagtcggca atgttgtctg cctgctccat aaaggtgaca aatttttctg
     7801 ctgcttccgt ctcttcggcc ttttgcccgg cggtaatggt cagcgaggtc aacatgccgt
     7861 agaccgcaga gtttttctcg gttggcacca cgaaaccgac gtttttcggg tcgccttctt
     7921 taattacagc cggaaggata taggtggagt aaatcgccat cggcgcggtg ccgttcataa
     7981 aggcgtcttt cacttccatg atgtcgttag aacccggcat gtcgttagaa cccggcatag
     8041 tgttggcagc aaggtcgcgg taataggtca gtgcctgcat catctctggt gtatcaaggg
     8101 tgattttgcc ttcggcgtta aagacattag cctggttgga taacgcaaac tgggagaagg
     8161 attgttccgt caacacgctt tctgctgtag gcagcgcaat gccgtatttt ttattcgccg
     8221 ggtcattcag tttctgtgca acgtccagta gctgttgcca gtttttcggc tcctccagcc
     8281 ccgcttttgc cagcacatct ttgcgatacc agatgccgcc aatccaggcg ctgacgggaa
     8341 caccggtcca ggcactccca tcttcggtgc gcacaatacg cagtacgcca tcgtaaaacg
     8401 cgccttcacc aacgttgctg atgaccgtgg caaccgtctt gcgatcgata agctgctctt
     8461 tatccataac tttggcgtag tcatggctgg tttcgatcac ttccggcagc gagccgctac
     8521 gtgaaagggt gatgactttg gtgttataag cgtcctcttc cacgggcact tgtttaacgg
     8581 tgatacctgg gttttctttt tcaaaacggg caatcaattt gctgataact gcctgacgct
     8641 cctgctccac cgaagaatgc ataaattcga tggatacatg ggttttattt tcttctttac
     8701 agcctgaaat cagggcgcag gaaaccagtg ctgataacag cacgattttt gatttaatca
     8761 tgagatgttc ctttttattt aatccacata acctgttagg ggcgcagcgt tatttcatta
     8821 cccaatagat caacttcact aatcagatcc cgaccatgca gattagtaat atttacatgc
     8881 tgaatatttt tactgacatt aaacaatcca gtcagacaat caccatcagc gttacttctt
     8941 tgaatacgca ttacggatga attaatcgta tcgatggtaa aattattatc cggatgaaac
     9001 tcgttatggc tgcgacgaag tgtaattaaa cgcgacaact catgatatac cgcatgcctt
     9061 aatgtagctt catcgttcag ttctcgggtt atctctttac tgtgatattt tttacggtta
     9121 atcgcacggt tatatccgag tttttcgaca cctgcgtaat cattacgcga gccaagaata
     9181 ctttgaatat atatcgctgg cacaccgggg aaacttaaca aaatcgcatg ggcgagaata
     9241 aacctggcgc aacgttcttc atcgctactc tcacggcggc ttaacgcatc catatgtgag
     9301 aggcgaggaa gttaaaccag gtggtgttgc tggaaggtag tgtcaggttt tgcgcccacg
     9361 cacaaagcgc ctcaacgttc tgtttttgca ccgcatgcag caccagtggc ggcagcgaga
     9421 actggtacac catatgtgct tcgtcatcgc ctgcgccaaa gtaagcaatg ttgtctttat
     9481 gcggaacatt ggtctcggta atgatcactg tacctggcgc aacgttatca ataatcgacc
     9541 gtaacagttt gataatcaga tgtgtttttt ccagatggat gcagcttgtt cccggctctt
     9601 tccacataaa gccaacggca tccaggcgga catactcagc acctttcgca aggtaacaga
     9661 gtaaaacatc caccatcgcc agcaacactt cagggctacg gtaattcagg tcaatttgat
     9721 cgtcactaaa ggtggtccat aaatggcgcg ttgaatgatc gcgcatctgg aatggcgtta
     9781 ataacggtaa cgcacgcggg cgagtgacgg cgctgagatc ggtttgcgga tcaaccgcaa
     9841 taaaaaaatc ttcaaaacct ggctgctgtt gtaaatagtt tttaaaccat tcactttttg
     9901 ccgacatatg gttgcagaca aaatcaaaca ttaaatgact gcattcaccg agttgctgaa
     9961 tatcctgcca ctcccccgct tcactggcga cctgatgata atcaattacc gaaaacccat
    10021 catcagatga acatggataa aaaggtaata aatgaacatg tgaaaaaatg ctttgcagcc
    10081 attgatggta aaactgatta aatgtgggta atggttttaa atcattgctg tgaaattgat
    10141 cggcataggt aattaacacg acatcacttt catcccagtg ttttttacgt cgctgtgtaa
    10201 ttaatcgttt cgcactctca agacgcgtta caagtttctg taaatgagtt gcggtaaatg
    10261 ttccaccgta gatttcgtcc aggtaatccg taattttctg tttcatgttg cctttttgtg
    10321 gtagcggtcc catgaaacga gagactactc cgcagaaaat aaactgtcaa ttgagcacaa
    10381 gggttttagt cacgcaggaa atcatttttt accagggaaa aagcgtattt gtgagctgac
    10441 gcaaataatc tatggggatg tgaataaggt gtgacagaaa aaaaacggcg cataagcgcc
    10501 gctcatggtg aattctttta acctttgacc ttcggcattg cgatgtcttt caggccaccg
    10561 gcgttctcaa cgtgggtata tcccatctcg ctaaggatct cttttgcttg ccctgactgg
    10621 cgtccggcat tgcaatacac tttcacggtg tcgtttttat ccggaacggc ggtggcaatg
    10681 cgctctttca cttccttcag gggaatattg atggccccct gaacgtgctc ttgctgatac
    10741 tgctctggaa cacgaacatc gatccagtgt tcagcggcga aaacgggcag tgaaaacacc
    10801 agtgccagag caagtaagcc ttttttaaac atgtaagcgt ccttctatgg atgaaaattg
    10861 ccatccatta tacgcctgag atttaacaat ttttgcgaaa ctcccctcac cttttataac
    10921 gctgtgccag tttattagca gcccgactta acagcggttc cagcgccaca gccagcaaca
    10981 ttttcagcgg tcggcgagca actgatttta tcgcccagcc cgctacaccc gccgggccat
    11041 agcgcagtgc ggtaagaagt accagcttgc ctgctaattt gaaaccaggc tttacctttt
    11101 gcccggcctg ttgccagcga gtattcataa ctttcctcac agttgacgga aacggctacg
    11161 taacgtgaaa gtatcggaag tgacataacg ttccatctcg cgtaaacgcg tttcacttgc
    11221 cgccagttca cggtcgactt catccagcaa ttcgctgctg gaaggtagct gctcaccaaa
    11281 ggccatgttg tccagcattg gatcaagcgc aaatgacaaa atgatgtaag caaccagggt
    11341 aaacagcgcc agaccgaaga aaatcgacag caccaccagg atacgcacca gttttaccgg
    11401 tacatcaaaa tagttggcaa tcccggcgca gacgccgcgg accatgccct gctgtggaat
    11461 acgccataat tttttattga gattaatgcc cgccattagc gatccctcca gttcggatgt
    11521 tctgcatcaa gaatagattc cagcgcctga atacgttcgc gcatccgttt tgcttcatca
    11581 gccagttgcg ctaatcgctg ctgctcactt tgcgacaatt catcgcgacc agaacgattg
    11641 ctgtaatgca gccataacca gatcggtaaa acaaacagca caaaaatggt taacggaata
    11701 gccagaaata gcgcgctcat aagtactcct tagacgatga gcggcggcgc gttcggacgc
    11761 cgcctggatg ttattattga ttgtcttgct tcattttggc ttttaattgt gccagttgtt
    11821 cgctgattgc atcatcggct ttcagttcgg caaactgatc gtccagcgat ttttgtttac
    11881 cgaagctgtg gctttctgct tccgcttcca tctggtcaat tcgacgttcg aaagattcga
    11941 aacgagccat tgcttcatcc agtttgccac tgtccagctg acgacgcaca tcgcgcgacg
    12001 agtttgccgc ctgatgacgt aacatcaatg cctgctggcg agcgcgtgtt tcgctcaatt
    12061 tgttttccag ctcgccaatc tctttcttca tgcgtgccag cgtatcgttc accagcgtca
    12121 cttcatgttc cagggactta atcagatcgg tcagtttctg tttttcaatt aacgctgcac
    12181 gtgccagatc ctctctctct ttcagcagcg ccagttcggc tttttcctgc cattcaactt
    12241 cacgcgccga cgcttgttca atacggcgag tcagctgttt cttttctgcc aacgcacgcg
    12301 ccgaagtaga acgtacttca accagtgtat cttccatctc ctggatcatc agacgcacca
    12361 gtttctgtgg atcttccgct ttctctaaca gagcgttgat gttggcattc acgatgtcgg
    12421 caaagcgaga aaaaataccc ataatgttgt cctcttgatt tctgcgttca ggattgtcct
    12481 gctgaactgt taatacaatt tgcgtgccaa ttttttatct ttttgattta taaagatctg
    12541 attgaagaat caacagcaac atgccaggat gaattagcga attacactaa caagtggcga
    12601 atttcatcat ggccgaatac aaagataatt tacttggtga ggcgaacagc tttctcgaag
    12661 tgctggaaca ggtttcgcat ctcgcaccgc tggacaaacc ggtgctcatc atcggcgaac
    12721 gcggcaccgg taaagagctg attgccagcc gcctgcatta tctctcctcc cgttggcaag
    12781 ggccgtttat ttcccttaac tgcgcggcgt taaatgaaaa tctgctggat tccgaactgt
    12841 ttggtcacga agcgggggcg tttaccggtg cgcaaaaacg tcatccaggg agatttgaac
    12901 gtgccgacgg cggtacgcta tttcttgatg aactcgctac ggcaccgatg atggtgcagg
    12961 agaaattatt gcgcgtgatt gagtacggtg aactggagcg cgttggcggc agccagccat
    13021 tgcaggtgaa tgtgcggttg gtatgcgcga cgaatgccga tctcccggcg atggtcaatg
    13081 aaggcacttt tcgcgctgac ctgctcgacc gactggcttt tgatgttgta caactgccac
    13141 cactgcgcga gcgcgaaagc gacataatgt tgatggcaga acactttgcc atccagatgt
    13201 gtcgggaaat caagctgcct ctgttcccgg ggtttacgga gcgcgccaga gaaacattgc
    13261 tgaattatcg ttggccggga aatattcgtg aattgaaaaa cgcggtggaa cgttcagtgt
    13321 atcgccacgg caccagcgat tatccgcttg atgacatcat tattgatccc tttaaacggc
    13381 gtccgcctga agacgctatc gccgtttcag aaaccacctc gcttccgacg ctgccgctgg
    13441 atttacgtga gtttcaaatg cagcaggaaa aagagttgct gcaactcagt ttgcaaaagg
    13501 ggaaatataa ccagaagcgc gcagctgaat tgctggggtt aacctaccat cagttccgcg
    13561 cgttgttgaa aaaacaccag atttagcgaa gttgggcaat gcgattcgcc ggatgcggcg
    13621 tgaacgcttt atccggcata cataatcaac gtcgtttgca ggcctgataa gcgcagcgca
    13681 tcagacatta ttgcgttggg ctattaatcg ctcagcgcat cctgcaaaat tttcattgcc
    13741 gcatcgaatt gcgcatccgg gatggtcagc ggatacagga agcgaatcac gttgccgtat
    13801 gcgccacagg tcagcaggag cagcccctgc gccagcgcgc gttgctggat tttctgtgca
    13861 atcgccgctg acggctcgcc cgtttgcgga tcgttaaact ctgccgcaat catcgacccc
    13921 aggccgcgta ccgcagcaat ggccggaacg ctttctttgg catcaatcaa cgtgtttttg
    13981 agacgctggc ccagttgatt cgcgcgttcg cagagtgatt ctttgttgat aatattgagc
    14041 accgcgtgcg cggcagccac cgccagcggg ttcccggcgt aggtgccacc caacccgccc
    14101 ggcgcgggcg cgtccataat attcgcgtta ccgaccacgc ccgaaagcgg catcccgccc
    14161 gcgaggcttt tcgccatcgt cattaagtcc ggcttatcgg cgtaatgatc catggcaaac
    14221 agtttaccgg tacgcgcaaa gccgctttgc acttcatcgg caatcatcac aataccgtgc
    14281 tcgtcgcaca ggcggcgaat ggcggcaacc agctcttttg gcgcaacgtt gaaaccgccc
    14341 tcgccctgca ccggttcgaa aataatggcc gcgacctgct tcgcttcgat gtctgattta
    14401 aacaagcgtt cgatggcgtc gagggagtcc tatgttgaaa tgccgtgtaa atctgacgga
    14461 taaggtacgt gatacaccga accagggaac gggccgaagc cgattttgta cggcgcaact
    14521 tttccggtca gcgccatggt catatatgta cgaccgtgga agccaccgct aaacgcaatc
    14581 acgccagggc gtccggtatg ggcgcgagca attttcaccg cgttttccac cgcttccgca
    14641 ccggtagtga agaacgcggt tttggcctgc ccgctcaccg gggcaagggc gttgattttc
    14701 tccgccaggg tgacgtagct ttcatacggc acaatctgat acgcggtgtg ggtaaactgt
    14761 tgcagttgct gctccaccgc cgcgaccaga tcagggtggc gatgtccggt attcagcacc
    14821 gcaatgcctg cggcgaaatc gatgtactcg ttgccctcaa catccttcag agtagcgttt
    14881 tcagccgact gggcgaagaa gttacacatc acgccaaccc cgcgtggggt agcagaaaga
    14941 cgacgctgat ggaattcatt gttgctcatg tttcacacct tttgactctt cactgttaat
    15001 cggaaattaa acgcccagac gatcgcgcag gctgtaccag gctgcgccca tcgcggtaaa
    15061 cggtactcgc agcgtgcgcc cgccggggaa cgggtaatac ggcagattgg cgaaggcatc
    15121 gaaacgttcg gcgtcgccgc gcagcagttc ggcaatcaaa cgtccggcga gatgagtaca
    15181 ggtgacgccg tggccgctgt aaccctgcat gtaatagatg ttggtatcaa ggcgaccaaa
    15241 ctgcggcatg cgcgacaggg tcagcaggaa gttgcccgtc cagcggtagt cgattttgac
    15301 gcccttcagt tgcgggaagg ttttcagcag tttcggcaca acaagacgct caacgtcatc
    15361 cgggtcgcgc gcgccgtaga ccacgccgcc gccgtacagc aaacggttgt cggcggtaag
    15421 acggtagtaa tcaagcagat aattacagtc ttccacacag tagtttttcg ggatcagcga
    15481 acgggctaaa tcttccgaca gccgttcagt ggtgatcacc tgcgtgccgc acggcatgct
    15541 gcgtttcgcc agttccggct ctactttatc gcccagatac gcattcccgg cgacaatcac
    15601 atacttcgcc gtcacctgac ctttggcagt gcgcaccacc gctggcgtgg tgtgctggat
    15661 ctgcgtcacg gcagaaagct catacacgcg cccgccgttc aggcggatgg catcggcttc
    15721 accaatggcg aggtttagcg ggtgaatatg cccaccgctg tgatccaaca gtgcgccagt
    15781 gtagcgatcg ctggcgacct cgcggcgaat ggcgttagcg tccagcagct ccagctgttt
    15841 attgccgtag cgttcccagt tctctttctg ctcttcgagt gtggcgagct gtttatcatt
    15901 catcgccaca aacaggccgc cggggcggta gtcgcagtca atctgatagc gtttgatacg
    15961 ttcgcggatg atctcgccac cctcgaacat catgctgccg agcatacggg cggtgtccat
    16021 gccgtagctt ttttcgatca cgtcgatgtc gcggctgtag gagttcacaa gctgcccgcc
    16081 attgcgcccg cttgcgccaa agccgatgcg tgaggcttcg agaaccacta cgtcaaagcc
    16141 cgcttccgcc agatgcagcg cggaggagag cccggtatag ccgccgccaa ccacgcaaac
    16201 gtcgcaggtg attgactcat tcagtgtgtc gaatggtgca tatttattcg cactggcggc
    16261 gtagtaactg ctggtatgtt cggtcatttc aggcctccag gcttatccag atggttttca
    16321 gttcagtgaa tttttcaagg gcatgcaggg atttgtcgcg accgttgccg ctctgcttat
    16381 agccgccaaa cggcacggtc atatcgccgt cgttgtagtt attgacgaag acggaaccgg
    16441 ctttcaggcg gcggctcata cggtgcgcgc gggagaggtc gcgcgtccat accgccgcgc
    16501 caaggccgta ctggctgtcg ttggcaagct gtagcgcctg gtcttctgat gtgaaacgcg
    16561 tgaccaccag caccggaccg aaaatctctt cgcggcttaa ggacgcattc gggtcaacat
    16621 ccacaaagat ggtcgggccg atggcggcag ccagctcagc gttacgacca tccagcagca
    16681 gttgcccttt gctttcgcct tcacgaataa agctatggac cgagtcggcg tgggcgcaat
    16741 cgattaaggt gcccatggtg gttacggtat caagtggatg gcccggctgc cagttttgcg
    16801 cctgctgttt taacagggct aagaattcat cggcgatgct ctcttccagt aacaaacgcg
    16861 ttccggcgat gcacacctgg ccctggttgt agaagatgcc tgcggcggtg gcgcttgccg
    16921 ctttttgcag atccggacaa tcggcgaaaa cgatgttggc gcttttgccg cccgcttcca
    16981 gccagacgcg tttcatgttg ctatcacccg cgtctttcag cagttgttta cccgtacgcg
    17041 tggagccggt aaaggcgatg gcatcgatat cgttatgacg cgaaagcgcc tgcccggctt
    17101 catgaccata accgctcact acgttcagca cgccatccgg cagccccgct tcttcttttg
    17161 ccagcccggc gagacgaatc gcactgagtg gtgatttttc agacggtttt agaatcacgc
    17221 tgtttcccgc cgccagcgcc gggccgagtt tccagcaagt cagcagcagc gggaagttcc
    17281 acggcacgat ggcggcaatc acgccgaccg gttcacgcac gatcatcgcc agctcatggc
    17341 tactggtggt cgccacttcg ccatacactt tgtcgatcgc ttcggcgtac cagcgaatgg
    17401 cgcgcgccgc gccgggaata tcatcacgca gactgtgacg aatcggtttg ccggtgtcga
    17461 gagtttccag cagtgccagc tcttcggcgt gggcttccat taaatcggcg agtttattca
    17521 gtaccgcttt acgtttagcc ggagaagaga gtgaccagtc gccgcgttca aatacgccgc
    17581 gtgctgcgct caccgcatgg tcgatatcga cgctcttgcc gcgggcaatt ttcgccagcg
    17641 gtgcctaggt gaccggatca acggtttcaa aggtttcatt ttccgccgca gcagtatatt
    17701 caccgttaat aaataagcgg ttttcaatgg cgagacttaa cgctttatcc tgccagtaag
    17761 ccagatgatg aaaattcatt atgactcctg tttcacgtct atcagatata tgcagtttgt
    17821 gaacgtcagg gcgcagagat ccccgggccg catgcccgtt tatttctggc gacattcttt
    17881 atggattgcc gggcgcattg cgcttacccg gcttacagaa caaaaagatt aaaacgtggt
    17941 gggcgtatgg gcgctgataa ttcggcaaat acctgccgac gtattactga aactgtgcgg
    18001 gatgccggta ttaatggcat aactttgccc cgcgacgagg tggtaatcct gaccattaat
    18061 cgtcagaaca atttcacctt ccagtactgt gcctatttcc tcaccctgat gcttaattct
    18121 ttccccagtg gttgtgcccg gctggtacgt ttcaaagatc atcgccagcg tgcgattcgg
    18181 gttaccgtta tgaaccagct tcattgacac accctgactg cccatctcaa ttaagtcgtc
    18241 ctgattaatg acgacctgcg gctcatcagg tttttccggc tcggaaaaga attccgagag
    18301 tgacagacca tacaccttca gcagcttttg cagcgtactg atggcagggc tgactttatc
    18361 ttgttctatc gtactgatag cactgtgagt cagcccggag agttcggcgg cacgacgttg
    18421 tgaaagcccc tgctgctggc ggatttccga caagcgtttt cctggcgcca gtccctcatc
    18481 actcatattt gcatttcctt aaactgtagt ggtcagagtc gctgtttttc agcgatatgg
    18541 tgctgacaag cggtgataaa gccctcgaac aatatacgcg aaagcgcgta ctcgctactg
    18601 ttccattccg ggtgccactg tacgcccagc gcaaaaggat gattgatgac gctaaccgcc
    18661 tcaactaagc catccggcga gcgagcttca acacgcaacc gtgggctaac gaccttcgcc
    18721 ccttgtccat gtagagagtt tacccaaaag ttgctacatt caggtaacaa cgcagacagt
    18781 aatcccccct cttcaacctg aacttcatgc gacggtgcat attgctgttc caccggcagt
    18841 tcgggatctt cccgatgttc cagcaattca ggctgttcgc acagcttgcg atgcaacgaa
    18901 ccaccggttg ccaccaccag ttcttgtaaa ccccggcaga tggcgaaaat ggggatgcgc
    18961 ctttcgagtg cggcattaat tatcgccatg ctcagaagat cacgcccggg atcggcgtca
    19021 ggctcatcgc cgttttcacc atataggtgc ggctgcacat tgctgggact accaggaaga
    19081 taaatgccat cgagtttcgg caaaagttgt tcaagtaacg acggttccgc cagcgcatgt
    19141 ggtagcgcaa ttggcaagcc gcctgcgtgg atgatggcat tcaggtactt ttcttgcaga
    19201 gtctgggtcg catgaccctt aagcctgttc ctgcacatta cgacaccgat aaccggattg
    19261 ttcattatat tttccatgct caatctcaca aagtggacta aattatcgcc attactgcgc
    19321 tttaaatgac ctttatgttc aatatttttt caatctagca gtgggttttc atttttgcaa
    19381 actcaattta acatttgaca aacatttagt ttgcatacag attcgaatgg tggtcattat
    19441 attttacgct ttgataacga gcggaaaaca aaccaaaggc gaagaatcat ggaaaccaat
    19501 atcgttgaag tagagaactt tgttcagcag tcagaagaga ggcggggtag cgcctttacg
    19561 caggaagtga aacgctacct ggagcgctac ccgaatacgc aatatgttga tgttttactt
    19621 actgatttga atggttgctt ccgtggaaaa cgtatccctg tttcaagcct gaagaagctc
    19681 gagaaagggt gttatttccc ggcctcggta tttgcaatgg atattctggg caacgtagta
    19741 gaagaagcag gtctgggtca ggaaatgggc gagccggatc gtacctgtgt tcctgttctc
    19801 ggttccttaa ctccttctgc cgccgatcca gagtttatcg gtcagatgct cctgaccatg
    19861 gtcgatgaag atggcgctcc ctttgacgtt gagccgcgga acgttcttaa ccgcctgtgg
    19921 cagcagctgc gccagcgcga cgctgaaggg aatctgcaac cgccctgcgc gcctggcacc
    19981 gatgaccgca atacacaaag ccaggtttac tccgttgata acctcaacca cttcgccgac
    20041 gtgctcaatg atattgatga actggcgcag ttgcagctga ttccggcaga tggcgcggtc
    20101 gctgaggcct cgccgggtca gtttgaaatc aacctttacc atactgataa cgtgctggaa
    20161 gcctgcgatg atgcgctggc actaaaacgc ctggtgcgtc tgatggcagg aaagcataag
    20221 atgcacgcca cttttatggc gaagccgtat gaagagcacg cgggcagcgg gatgcatatc
    20281 catatcagta tgcaaaataa tcgtggcgag aatgtgcttt ctgacacaga aggcgaagat
    20341 tcgccgctgc tgaaaaagat gctcgccggg atgattgacc tgatgccgtc gtcgatggcg
    20401 ttgctggcac caaacgtgaa ctcgtatcgc cgcttccagc cgggaatgta tgtgccgacg
    20461 caggcgtcgt ggggccataa caaccgcacc gtcgccctgc gtattccgtg cggcgaccgt
    20521 cataatcacc gcgtggaata tcgcgtggcg ggtgccgatg ccaacccata tctggtgatg
    20581 gcagcgattt ttgccggtat tttgcatggc cttgataacg agctgccgtt gcaggaagaa
    20641 gtcgaaggca acgggctgga acaggaaggc ttacccttcc cgattcgcca gagcgatgcc
    20701 ctgggtgagt ttatcgagaa tgatcacctg cgccgctatt taggcaaacg gttttgccat
    20761 gtgtatcacg cctgtaaaaa tgatgagctg ttgcagtttg agcgcctcat cacagaaaca
    20821 gaaattgagt ggatgttgaa aaacgcgtaa ttccccttgc ggggttgttt catcggcctg
    20881 agcggagcca ggcctgacat ttgctggtaa tccagacatt tcccggatgt cgcgttatgg
    20941 agcgcaggcg gcaacgggct tccgttaacg attaagactg tgacgaggaa atgagatgat
    21001 gccgatgatt aaatccccgc atggcgaggg aggctgcgta tgcgcccctc ctgctactga
    21061 ctggactccg ccccctttgc ttcccttgct aaacaggttt gatttcaggt cgacacgacc
    21121 gcaaacctta ttacgcaggg gaggcagcaa ttatggctat taattcacca ctgaatattg
    21181 ctgcgcaacc cggcaaaacc cgtctgcgaa aatcactgaa attgtggcag gtggtaatga
    21241 tgggtctggc ctatctcacg ccgatgaccg tatttgatac ttttggtatt gtctccggca
    21301 ttagcgacgg tcacgttccg gcgtcctatt tgctggcgct ggcgggcgtg ctgtttaccg
    21361 ctatcagcta cggcaaactg gttcgccagt ttccggaggc cggttcggcc tatacctacg
    21421 cgcaaaagtc gattaacccg cacgtcggat ttatggtcgg ctggtcatca ctgctggatt
    21481 atctcttttt gccaatgatc aacgttttgt tggcgaaaat ctatctctcc gccctcttcc
    21541 cggaagtgcc gccgtgggtg tgggtggtaa ccttcgtcgc cattttaacc gccgcgaatc
    21601 tgaagagcgt caacctggtc gctaacttca ataccctgtt tgtactggtg caaatctcca
    21661 tcatggtggt gtttatcttc ctggtggttc agggactgca taaaggagaa ggcgttggca
    21721 ccgtctggtc acttcagccg tttatcagcg agaacgcgca cctgatcccg attattaccg
    21781 gtgcgacgat tgtctgtttc tcgttcctcg gtttcgatgc ggtgaccacg ctttcggaag
    21841 agacgccgga cgccgcacgc gtgatcccga aagccatctt cctgacggcg gtatacggcg
    21901 gcgttatctt tatcgcggcg tcgttcttta tgcagctgtt ctttcccgat atcagccgct
    21961 ttaaagaccc ggacgccgca ctgcctgaaa ttgcgctgta cgtcggcggc aagctctttc
    22021 agtcgatttt cctctgcacc acgtttgtga acacattagc gtctggcctg gcttcacatg
    22081 ccagcgtgtc gcgtctgctg tatgtgatgg ggcgtgacaa tgtgtttccg gagcgcgtgt
    22141 ttggctatgt gcacccaaaa tggcggactc cggcactcaa cgtcattatg gtcgggattg
    22201 tcgcgctgtc ggcgctgttc tttgatttag tcaccgcgac agcactgatt aacttcggtg
    22261 ctctggtggc gtttaccttc gtgaatctgt cggtgtttaa tcatttctgg cggcgtaaag
    22321 gaatgaataa aagctggaag gatcacttcc actatttgct gatgccgctg gttggcgcgc
    22381 tgacggtggg tgtgctgtgg gttaacctag agtcaacgtc actgacactc ggtctggtat
    22441 gggcttcgtt aggcggcgca tatttgtggt atctgatccg ccgctatcgc aaagtgccgc
    22501 tgtacgaagg tgacagaacg ccggtgagcg aaacgtaata gccggatgcg cggcgcatta
    22561 tcctgcctga gggtaacgcg ccgcgccatt aactataaaa agatgtataa cccggatcac
    22621 gagtgctatc atccgccctt atgcttagcg cattaacccc aggggcaaat aatgaaccac
    22681 gatatcccgc taaaatattt tgatattgcc gatgagtacg cgaccgaatg cgcagagcct
    22741 gtcgcagagg ctgaacgcac accgctggcc cactacttcc agttactgct cacccgtttg
    22801 atgaacaatg aagagatcag cgaagaagcc caacatgaaa tggctgctga agcggggatt
    22861 aatcccgtac gcattgatga gatcgcagag tttttgaatc aatggggtaa tgagtaatta
    22921 tttaattgca gacgatcggt cagtatcgtt gtccggataa agcgttcacg ccgcatccgg
    22981 ctaacaatga cacgcactgc agcgtgcttt aatgggtttc tcacgatcgt accgctctgc
    23041 tcctcgctaa gttgttctgt gagttatagt tatagagaaa tcacgggtac gaatgttgaa
    23101 cagatggcga cgccacctgt tcaacgacca accacacggc cctcattacg acccctgaac
    23161 acaaacggta atggcaaatg tcattaaata ttagtaagat agcctcttta cataaaatcc
    23221 cttaatatta tgcgccaggt attatcgtct cttttggtga ttgctggact tgtgagtggt
    23281 caggcaatcg ccgcgcctga atcccccccg catgctgata tccgcgacag cggttttgtc
    23341 tattgcgtca gcgggcaagt caacaccttt aacccatcca aagcgagcag tgggttaatt
    23401 gtcgataccc ttgccgccca gttttatgat cgactgctgg atgtcgatcc ctatacctat
    23461 cgcctgatgc cggaacttgc cgaaagctgg gaagtactcg acaacggcgc gacctatcgc
    23521 ttccacctgc gtcgcgatgt tccgtttcaa aaaaccgact ggtttactcc cactcgtaaa
    23581 atgaatgccg acgatgtggt gtttaccttc cagcgaattt ttgaccgcaa caacccgtgg
    23641 cataacgtca acggcagcaa cttcccctac ttcgacagcc tgcaatttgc cgataacgtg
    23701 aaaagcgtcc gcaaactgga taatcatacc gttgagttcc gactcgccca gccggatgct
    23761 tcttttttgt ggcacctcgc aacccattat gcttcggtca tgtcggcaga atatgcccgg
    23821 aagttagaga aagaagatcg ccaggagcaa ctcgaccgtc aaccggtcgg caccggtccg
    23881 tatcagttgt cggaataccg cgccgggcaa tttattcgcc tacaacgtca tgatgacttc
    23941 tggcgcggta aaccgttaat gccgcaggta gtggtggatt taggctccgg cggcaccgga
    24001 cgtctgtcga aactcctgac cggggaatgc gacgttctgg cctggcctgc tgccagccag
    24061 ctatccattt tgcgtgacga cccgcgcttg cgtttaacgc tgcgtcctgg gatgaacgtc
    24121 gcctatctgg catttaacac cgccaaaccg ccgctaaata atcccgctgt ccgccatgca
    24181 ctggcactgg cgattaataa ccagcgcctg atgcaatcca tctattatgg tacggctgaa
    24241 acggcggcct ctattttacc gcgcgcctcg tgggcctatg acaacgaggc taaaattact
    24301 gaatacaatc cggcgaaatc gcgcgaacag ttgaaggcgt tggggctgga aaacttaacg
    24361 ctgaaactgt gggtgcccac acgttcgcag gcgtggaacc ccagtccact gaaaactgcc
    24421 gaactgattc aggcggatat ggcgcaggtt ggcgtaaaag tggtgattgt gccggtagaa
    24481 ggtcgctttc aggaggcgcg gttgatggat atgagccatg atctgacatt atccggttgg
    24541 gcgacggaca gtaacgaccc ggacagtttc ttccgtcctt tactgagctg cgcggcaatt
    24601 cattcacaga ccaacctcgc ccactggtgc gatccgaaat tcgacagcgt gttgcgtaag
    24661 gcgctctcct cgcagcagct ggcggcgcgt attgaagcct atgacgaagc gcagagtatt
    24721 ctggcgcagg aattacctat tctgccgctg gcgtcgtcat tgcgtttgca ggcctatcgg
    24781 tacgatatca aaggtctggt acttagcccg tttggtaacg cctcctttgc tggggtgtat
    24841 cgcgagaaac aggatgaggt gaaaaaacca tgattatctt caccttacgc cgcattttgt
    24901 tattgattgt caccttgttc ctgctgacct tcgttggctt cagcttgagc tatttcaccc
    24961 ctcatgcgcc gttacaaggc gcgtcactgt ggaatgcctg ggtgttctgg tttaacggcc
    25021 tgatccactg ggattttggt gtttccagca ttaacggtca accgattgcc gaacagttaa
    25081 aagaggtctt cccggcgacg atggaactgt gcatcctcgc cttcggtttt gcgctgattg
    25141 ttggcatccc cgtggggatg attgccggga ttacgcgcca taagtggcag gataacttga
    25201 tcaacgccat cgccttactg ggcttttcaa tcccggtgtt ctggctggcg cttctgttga
    25261 cgctgttttg ttcactcacg ctaggttggt tgcccgtttc agggcgtttc gatctgctct
    25321 acgaagtgaa accgattacc ggttttgcgt tgattgatgc ctggctttcg gactcaccat
    25381 ggcgggatga aatgatcatg agcgcaatcc gccatatgat attgcccgtg atcaccctgt
    25441 cggttgcgcc aacaactgaa gtgatccgcc tgatgcgtat cagcactatc gaagtgtatg
    25501 accagaacta tgtaaaagcg gcggcgaccc gcggtttgtc gcgctttact attttgcgtc
    25561 gccacgttct gcataacgcg ttaccgccgg ttattcctcg cctgggctta cagttttcca
    25621 ccatgctgac gctggcgatg atcaccgaaa tggtttttag ctggccgggt ttagggcgct
    25681 ggttaattaa cgccatccgc cagcaggatt acgcagccat ttccgccgga gtgatggtgt
    25741 gtggctcact ggtcattatt gttaacgtga tttctgatat tttgggtgcc atggctaacc
    25801 ctctgaaaca taaggaatgg tatgccttac gatagcgtat acagcgaaaa gcgcccgccg
    25861 ggcacgctgc gtaccgcctg gcgcaaattt tatagtgacg cctctgcgat ggtcggcctg
    25921 tacggctgcg cggggctggc tgtactgtgt atttttggcg gctggtttgc gccttacggc
    25981 atcgaccagc aatttctcgg ttatcaatta ttgccgccat catggtcgcg ctatggcgaa
    26041 gtttctttct tcctggggac tgacgacctc ggacgcgatg ttttaagccg tttactgagc
    26101 ggagctgcgc caaccgtggg tggcgcattt gtggtgacgc ttgccgcgac gatctgtggc
    26161 ctggtactgg gcaccttcgc cggggcgaca cacggcctcc gctcagcggt gcttaaccat
    26221 attctggata ccttactggc gatcccttcg ctgctgttgg caattatcgt tgtagcgttt
    26281 gccggaccga gtttgtccca cgctatgttt gctgtctggc tggcattgct gccgcgtatg
    26341 gtacgttcga tttacagcat ggtgcatgac gaactggaaa aagagtacgt tatcgccgcc
    26401 cgtctggatg gcgcatcaac gctgaatatt ctctggtttg ctgtgatgcc aaacatcacc
    26461 gccgggctgg tcaccgagat cacccgcgca ctgtcgatgg caattctcga tatcgccgcg
    26521 ctgggctttc tcgatctcgg cgcacaactc ccctcgcctg aatggggagc gatgctcggt
    26581 gatgcgctgg aactgattta tgtcgccccg tggactgtca tgctgccagg tgcggcaatt
    26641 atgattagcg tgttgttggt taacctcctt ggcgatggcg ttcgtcgtgc gattattgcg
    26701 ggggtggaat aatgccatta ctcgatattc gtaacctgac cattgaattt aaaaccggtg
    26761 atgagtgggt taaagccgtc gaccgggtaa gtatgacctt aaccgaaggt gaaatccgcg
    26821 gtcttgttgg tgaatccggt tccggcaaga gtttgattgc gaaagcgatt tgtggggtga
    26881 ataaagataa ctggcgtgtt actgctgacc gtatgcgttt tgatgatatc gatttgctgc
    26941 gtctctccgc acgcgaacgg cgcaaactgg ttggccataa cgtgtcgatg attttccagg
    27001 aaccgcagtc gtgtcttgac ccttcagaac gtgtgggtcg ccagttgatg caaaacatcc
    27061 cagcctggac ctataaaggc cgttggtggc agcgttttgg ctggcgcaaa cgccgtgcca
    27121 ttgaactgct gcaccgcgtg gggattaaag atcacaaaga tgcgatgcgc agttttccct
    27181 atgagttgac cgaaggtgaa tgtcagaaag tgatgatagc cattgcactg gcgaatcaac
    27241 cgcgtctgct gattgctgac gaaccgacca actcaatgga gccaacaacc caggcgcaaa
    27301 tctttcgcct gctgacgcgt ctcaaccaaa acagcaatac cactattttg cttatcagcc
    27361 atgacttaca aatgcttagc caatgggcgg ataaaattaa cgtgctttac tgcggtcaaa
    27421 cagtggaaac cgcgccgagt aaggagttgg tgacgatgcc acatcatcct tatacccagg
    27481 cgctgatccg cgcgatacca gacttcggca gcgcgatgcc gcataaaagt cgcctcaata
    27541 cgctgcccgg cgctatccca ctgctggaac agttaccgat tggttgtcgt ctggggccac
    27601 gttgcccgta tgcacaacga gaatgcattg tgacgccacg tttgacgggg gcgaaaaatc
    27661 atctctatgc ctgtcatttc ccgctgaaca tggagaaaga gtgagatgat cgaaacgctg
    27721 cttgaagtgc gtaatttaag taaaacgttc cgctaccgga ccggctggtt tcgtcgtcag
    27781 accgtagaag cggtaaaacc cttgagcttt acgctacgtg aaggccagac actggcgatt
    27841 attggcgaga atggttcggg taaatccacg ctggcaaaaa tgctggcggg aatgatagag
    27901 cctaccagcg gcgagttatt gattgacgat catccactgc attttggcga ttattccttc
    27961 cgcagtcagc gtattcgcat gatttttcag gatccttcga cctcgttgaa tccccgtcaa
    28021 cgtatctcgc aaattctgga ttttccactg cgcctgaaca ccgacctgga accggaacag
    28081 cgtcgcaaac agattattga aacgatgcgt atggttgggc tgctaccgga tcacgtcagt
    28141 tactatccgc atatgttggc acccggacaa aaacagcgtc tgggtctggc gcgcgcgcta
    28201 atattgcgcc caaaagtcat tattgccgat gaagccctcg cttcactgga tatgtcgatg
    28261 cgttcgcagt tgattaatct gatgctggag ttacaggaaa aacagggcat ttcgtatatt
    28321 tatgttaccc agcatatcgg aatgatgaag cacattagcg accaggtgct ggtgatgcat
    28381 cagggcgagg ttgtcgagcg aggcagcacc gcggatgtgc tggcatcgcc gctgcatgaa
    28441 ctcaccaaac ggctgattgc cgggcacttt ggcgaagcat tgacagcgga tgcgtggcgt
    28501 aaagatcgct aattttctga atcctccttc cagattatcc acctcattct gacaagagat
    28561 taaatcttgt cagaatgagg tgatggataa aataaaatca aatgcgcgtg atttaagacg
    28621 caatctcact ttgcaggaac gaaagctctg gcgatatctt cgcagccgac gttttggtga
    28681 tttcaaattt cgccgtcaac atccggtggg gagctacatt ctcgattttg cttgctgctc
    28741 ggcgcgcgta gtcgttgagc tggatggtgg gcagcatgat ttggcagttg cctatgatac
    28801 caggcgcact agctggcttg agtcgcaggg ctggaccgtg ctgcgtttct ggaataacga
    28861 gattgattgt aatgaggagg cggtgccgga gattattctg caggaactga accgccgatc
    28921 accctcaccc tgaaagggcg agggggccga ccgagccgaa tagctgttgt ggtgaaaaca
    28981 tggagacagt gctggagaat attcggcaag gtctgaacca tcccagccat cgccataaaa
    29041 gggttagggg ctgtatgagc ctgtttgttg ctggggtaac aatatttgca caatatggtc
    29101 ccctcgcccc tctggggaga gggttagggt aaggggaaaa gcgcccaccc tgccgcagcc
    29161 tgctccggtc ggacctggca actatagcta ctcacagcca ggttgattat aataaccgtt
    29221 tatctgttcg tactgtttac taaaacgacg aatcgcctga ttttcaggca caacaagcat
    29281 caacaataag gattaaagct atgggttttc tttccggtaa gcgcattctg gtaaccggtg
    29341 ttgccagcaa actatccatc gcctacggta tcgctcaggc gatgcaccgc gaaggagctg
    29401 aactggcatt cacctaccag aacgacaaac tgaaaggccg cgtagaagaa tttgccgctc
    29461 aattgggttc tgacatcgtt ctgcagtgcg atgttgcaga agatgccagc atcgacacca
    29521 tgttcgctga actggggaaa gtttggccga aatttgacgg tttcgtacac tctattggtt
    29581 ttgcacctgg cgatcagctg gatggtgact atgttaacgc cgttacccgt gaaggcttca
    29641 aaattgccca cgacatcagc tcctacagct tcgttgcaat ggcaaaagct tgccgctcca
    29701 tgctgaatcc gggttctgcc ctgctgaccc tttcctacct tggcgctgag cgcgctatcc
    29761 cgaactacaa cgttatgggt ctggcaaaag cgtctctgga agcgaacgtg cgctatatgg
    29821 cgaacgcgat gggtccggaa ggtgtgcgtg ttaacgccat ctctgctggt ccgatccgta
    29881 ctctggcggc ttccggtatc aaagacttcc gcaaaatgct ggctcattgc gaagccgtta
    29941 ccccgattcg ccgtaccgtt actattgaag atgtgggtaa ctccgcggca ttcctgtgct
    30001 ccgatctctc tgccggtatc tccggtgaag tggtccacgt tgacggcggt ttcagcatcg
    30061 ctgcaatgaa cgaactcgaa ttgaaataat cattctgttg gtaaatattg ggcggcgttc
    30121 tgccgcccgt tatctctgtt atacctttct gatatttgtt atcgccgatc cgtctttctc
    30181 cccttcccgc cttgcgtcag gataacgatt tcctttacga ccaaggagcg cccatggaac
    30241 aacgccacat caccggcaaa agccactggt atcatgaaac gcaatccagt actgcggagt
    30301 atgacgttct gcctctggtc ccggaagccg caaaggtcag cgatcccttt ctgctcgacg
    30361 tgatccttga tgaagaaacg ctggctccct tcctttcatg gctggtccct gcgcgcgttc
    30421 ttgcagtgga attgttccct gaccagctta ccgtgacccg ttcacagacc ttcaccgctt
    30481 atgaacgctt gtctacggcc ctgacggttg ctcaggtttg cggcgtccag cggttatgta
    30541 actactattc ggcgcgactt acgccgctcc ccgggcctga ttcctccagg gaaagcaatc
    30601 ataggttggc acaaatcacg caatatgccc gccaactggc tagctcgcct tctattatcg
    30661 acaaccgatc gcgccagcat ctgaatgacg tcggtcttac tgcctgggac tgtgtgatca
    30721 ttaaccaaat cattggtttt attggctttc aggcgcggac cattgcgaca tttcaggctt
    30781 atctcgggca cccggtacgc tggttacccg gtctggaaat acaaaactac gccgacgcgt
    30841 cactgtttgc tgatgaatca atacgctggc gaagcagcta tgaggtggaa aaactacctg
    30901 aagagtacac aaaaagttca acagcagaac tttgccaact ggccgaaaca ctctctctcc
    30961 accctatttc actttccctt ctcgaaaggt tgttaaacag tacacgggtt aatacacagc
    31021 cggataataa gcttgcggcg ttgttatgcg cgcggataaa tggcagtcct gcttgttttg
    31081 ccgcctgtat ggattcatca aatgaatata aaaaatcagc ccccttctgc gcaagggcga
    31141 aaatgaaatt aaccaatggg ctgaccgtca ttctgttgag cacgctaccg ttcaggcgat
    31201 acaatggctg acccgagcac ccgatcgctt tagcgccgcc cagttcagcc ctttactcgg
    31261 acacgaaaaa tcatcaacgc agattattaa tctgctggta tggagcgggc tgtgtggctg
    31321 gataaatcgc ttaaaaatcg cgttgggtga gacatattaa ccttgccgcg tcagacagat
    31381 tcgcgtaaaa ctgtcagccg ctctaatggc caccaaaata gacaattatg tttcaggaca
    31441 acccgctgct agcgcagctt aaacagcaac tgcattccca gacgccacgc gctgaagggg
    31501 tggtaaaagc cacagaaaaa ggctttggct tcctggaagt cgacgcgaaa aaaagttatt
    31561 tcattccgcc gccgcagatg aaaaaagtca tgcatggcga ccgaattatc gcggtgatcc
    31621 acagtgaaaa agaacgtgaa tccgcagagc cagaagaact ggttgaaccg ttcctgactc
    31681 gtttcgtggg taaagttcag ggcaaaaatg accgtctggc catcgttcct gatcatccac
    31741 tcttaaaaga cgccattcct tgccgcgcag cccgtggcct gaaccacgag tttaaagaag
    31801 gcgactgggc ggttgccgaa atgcgccgtc atccgctgaa aggcgatcgt tctttctatg
    31861 ctgaactgac acaatacatc acttttggtg acgaccactt tgtaccgtgg tgggttaccc
    31921 ttgcgcgcca taatctggaa aaagaagcac cagacggcgt cgctaccgaa atgctcgatg
    31981 aaggtctggt tcgtgaagat ctgaccgcgc tggattttgt caccatcgac agtgccagca
    32041 cagaagatat ggatgacgcc cttttcgcta aggcgttgcc ggatgacaaa cttcagctga
    32101 ttgtggcgat tgccgatcca accgcgtgga ttgctgaagg cagcaagctg gacaaagccg
    32161 cgaaaattcg cgcattcacc aactatctgc ctggcttcaa catccctatg cttcctcgcg
    32221 agctttctga cgatctctgc tcactgcgcg ccaatgaagt ccgcccggta ctggcatgcc
    32281 gcatgacgct ctccgctgat ggcaccattg aagataatat cgaattcttt gccgccacca
    32341 tcgaatccaa agcgaagctg gtgtatgacc aggtttctga ctggctggaa aataccggtg
    32401 actggcagcc tgaaagtgaa gcaattgccg aacaagtccg tttgctagcg caaatttgcc
    32461 aacgccgcgg cgagtggcgt cataaccacg cactggtgtt taaagatcgc ccggattacc
    32521 gctttattct cggtgaaaaa ggtgaagtgc tggatatcgt cgccgagcct cgtcgcattg
    32581 ccaaccgtat cgtcgaagaa gcgatgattg ccgctaacat ttgtgcggcc cgcgttctgc
    32641 gcgataagct cggttttggc atctataacg tgcatatggg ctttgatccg gcgaatgccg
    32701 acgcgctggc agcgttgctg aaaacgcacg gtctgcatgt cgatgccgaa gaagtgctca
    32761 cgctggacgg tttctgcaaa ctgcgtcgtg aactggatgc gcaaccaact ggtttcctcg
    32821 acagccgcat tcgtcgcttc cagtcatttg ctgaaattag cactgaaccc ggtcctcact
    32881 ttggcctcgg tctggaagca tacgccacct ggacttcgcc gatccgtaaa tatggcgaca
    32941 tgatcaacca ccgtctgctg aaagcggtta tcaaaggcga aactgcgacg cgtccgcagg
    33001 atgaaatcac tgtccaaatg gccgaacgtc gccgtctcaa ccggatggca gaacgtgatg
    33061 ttggcgactg gttatacgca cgcttcctga aagataaagc cgggaccggc acccgtttcg
    33121 cggcggaaat tgtcgatatc agccgtggcg gcatgcgtgt gcgtttggtt gataacggcg
    33181 ctatcgcctt tattccggca cctttcttac acgctgtgcg cgatgaactg gtttgcagcc
    33241 aggaaaacgg cactgtacaa attaaaggtg aaacggttta caaagtaact gacgttattg
    33301 acgtcaccat tgccgaagtc cgcatggaaa cccgcggcat tattgcgcgc ccggtcgcgt
    33361 aatctccttt cacggcccat tcctcatgga tgggccgttt atttccccgc tctaccttcg
    33421 ttatatccag gcagattttt taactgactt tcgtttgaaa actggcgttt ttccaaaact
    33481 ggattacaaa taaatatata aaaaaacaat gcgtttagcc gccgttaatt tcatgtaagt
    33541 catgttgttc cttttattta accgcggact ccgcagttaa ccggaggata tgcatcatga
    33601 aaaccgttag ggagtccaca acgttgtaca actttctcgg atcgcacaat ccatactggc
    33661 ggttgacgga aagcagcgat gttttgcgct tttttaccac cgaaaccaca gaacctgatc
    33721 gtactttgca gttatctgcc gaacaggctg ctcgcatcag ggaaatgacg gtcatcacct
    33781 ccagcctgat gatgagtctg accgtcgatg agagcgatct ttctgtgcat ctggtaggac
    33841 gaaaaatcaa taaacgggaa tgggctggca acgcgtctgc atggcatgac acaccggcag
    33901 ttgctcgtga tttatcacac gggctttcct ttgctgagca ggtagtttct gaagcacatt
    33961 ccgcaatagt gattctcgac agccggggga atatccaacg cttcaatcgg ttatgtgaag
    34021 attacactgg gttgaaagaa cacgacgtca ttggccaaag cgtgtttaaa ctgtttatga
    34081 gccgtcgtga agctgcggca tccaggcgca ataaccgtgt attttttcga agcggcaatg
    34141 catatgaagt cgaactgtgg ataccaacac gtaaaggcca gcggctgttt ctgtttcgca
    34201 ataaaataaa tttgtccaca gcggcagtgg caaaaacgag atttttttaa tctgttccgg
    34261 taccgacatt accgaagagc gccgcgctca ggagcgactg cgtattctgg caaataccga
    34321 cagtatcacc ggactgccga atcgtaacgc aatgcaggag ttaatcgatc acgctattaa
    34381 tcaggctgat aacaataaag ttggggttgt gtatcttgat ttggataatt ttaaaaaggt
    34441 caacgacgcc tatgggcatt tgtttggtga ccagttatta cgcgacgtgt cattggctat
    34501 tttaagctgt ctcgaacatg accaggtgtt ggcgcgtcca ggtggcgatg agtttctggt
    34561 actggcatcc aatacctcac aaagcgcgct ggaagcaatg gcatcacgaa ttttgacccg
    34621 cttacgcctc ccctttcgta ttggtttaat tgaagtttat accagctgtt cagtaggtat
    34681 ttcgctctct cccgaacatg gttcggacag cgcgactatt attcgtcacg ccgacacagc
    34741 aatgtacaca gcgaaggaag gcggacgagg acaattttgc gtttttactc cagaaatgaa
    34801 tcagcgggta tttgaatatc tctggctgga taccaacttg cgtaaagcac tggaaaacga
    34861 tcagttggtt attcactatc aaccgaaaat cacctggcgt ggcgaagtgc gcagtctgga
    34921 agcactagta cgttggcagt cacctgaacg tgggttgatt ccaccgttgg acttcatttc
    34981 ctacgccgaa gagtcagggc taattgtgcc tttaggccgt tgggtgattc tcgatgtcgt
    35041 acgccaggtg gcaaagtggc gggataaagg cataaacctg cgagtggcgg taaatatttc
    35101 tgcacgtcag ctcgccgatc aaaccatttt caccgccctg aaacaggttc tccaggaact
    35161 caattttgaa tactgcccta tagatgttga actgacagag agttgtctga ttgagaatga
    35221 tgaactggca ctgtctgtta ttcaacaatt tagccaacta ggtgcgcaag tgcatctgga
    35281 tgattttggt accggctact cttcactttc gcaactggcg cgctttccga tcgatgccat
    35341 caaacttgac caggtttttg ttcgagatat tcacaaacaa cctgtctcgc agtcactggt
    35401 ccgggcgatc gtcgctgtgg cccaggcatt gaatcttcag gtgatcgccg aaggtgtaga
    35461 gagtgcaaag gaagatgctt ttttaaccaa gaacgggatc aatgagcggc aaggattttt
    35521 gtttgccaaa ccgatgcccg ccgtcgcctt cgaacgctgg tataaacgct atctgaagcg
    35581 cgcataagta gatgaatata tatacttagc gctatatccc gtcattatat atcgtattcc
    35641 tggatgataa ttagccgttg ctgttaaaca cttctctttt agcggcaatt tttgcattat
    35701 tcattttcca cttttgctat aaggatgtcc ttgatgtctg aatttgatgc ccagcgcgtt
    35761 gccgaacgta ttgatattgt gttagatatc cttgtggcag gcgattacca ctctgcgatc
    35821 cataatctcg agatacttaa agcagaactc ttgcgccagg ttgctgaatc tacgccagac
    35881 atcccgaagg caccgtggga gatttaacaa tcgtgccttc aggttttcct atttgaggat
    35941 gcccggcgcg tcagtatttt tttatttagt attataacgt tataagaatt acagcgatga
    36001 attcccgaca acaaactatt ctacagatgg tcattgacca gggtcaggtt agcgtaaccg
    36061 atctggcaaa agccactgga gtttctgaag ttaccattcg ccaggatctc aacaccctcg
    36121 aaaaactgag ttacctccgc cgtgcacatg gctttgcagt ttcgcttgat agtgatgacg
    36181 tcgaaacccg tatgatgagc aactatacgc tgaagcgtga actcgccgag tttgccgcgt
    36241 cactggttca accgggcgaa accatcttta tcgaaaatgg cagcagcaat gccctgctcg
    36301 cccggactct gggggagcag aagaaaaatg tcactatcat cacggtcagc agctacatcg
    36361 cgcatttgct gaaagacgcg ccttgtgaag tcattttgct cggtggcgtg taccagaaaa
    36421 aaagcgaaag tatggttggc ccgttgacac gccagtgcat ccaacaggtg catttcagca
    36481 aagcatttat tggtattgat ggctggcaac ctgaaactgg attcaccggt cgcgatatga
    36541 tgcgtaccga tgtggtcaat gccgtgctgg aaaaagagtg cgaagcgata gtcctgactg
    36601 acagctcgaa atttggtgct gtacattcat actccatcgg tcccgttgag cgattcaatc
    36661 gcgtgattac cgattcgaaa atacgcgcca gcgatctgat gcatcttgag cacagtaaac
    36721 tcaccgttca cgtcgttgac atttaaccat ttcccttctc tacggatgat ttgcagtttg
    36781 gcaaatcatc cgctctaaga tgattcctgg ttgataatta agactattta cctgttatta
    36841 acacgcttaa gatataaaat tattatcagc gatataacag gaagtcatta tcacctgcgt
    36901 gatataaccc tgcgcgcgag cagatttcac ggaataattt caccaggctt attcttagct
    36961 attatagtta tagagagctt acttccgtga atcataaatt caggagagag tattatgttt
    37021 gtaacgagca aaaaaatgac cgcggctgtt ctggcaatta ctttggcaat gtctctgagt
    37081 gcctgttcta actggtctaa acgggaccgc aacaccgcaa tcggcgcggg tgcaggggca
    37141 ttaggcggtg cagtactgac cgatggcagt acgttgggta cattaggtgg tgcagctgtc
    37201 ggtggtgtta ttggtcacca ggtcggtaaa taatcgtaaa taaatgtcga ttatcgactc
    37261 gcaattataa tacgcttgat tctgttcagt acgaaataac tgaataatac gcacagtaaa
    37321 atattcgaaa gccacggata tatccgtggc tttttatgtt aaccgcctgc gagttttacc
    37381 ttcatccctt tcgcttcaag tagtgatttt aataaatcac gcttatcgcc ctggatttca
    37441 ataactccat ctttaactgc tccgccgcag ccgcattttt tcttcagttc cgctgcaagt
    37501 tttgtcagtt cggcatcatc gagatcgaca ccggtaatca ggcaaacgcc cttaccttta
    37561 cgtccactgg tctgacgctg aatacgcacc acaccgtcgc ctttagggcg tacaggggcc
    37621 gctttgggct catcaatacg tccggtttcc gttgagtaga ccagacggct gttggaatca
    37681 ctcatcatgc actccgctgt aaagaggcgt tgatcgcttt cagcgtctgc gctggatcta
    37741 ccgattgcgt taccgggcga ccaatcacca tataatcaac accagccgac aacgcctgtt
    37801 ctggcgtcat aatgcggcgc tggtcaccag cttcactccc ctgcggacga atgcccggcg
    37861 taaccagttt gaactcctga ccgaatacct gtttaaagcg cacagcttcc tgagcagaac
    37921 acaccacacc atcaaggcca catttttgcg tcagtgccgc cagacgttct gcataatctg
    37981 caggtgacag tgtcatgcca agatcgacca ggtcgctggc ttccatgctg gtcaacactg
    38041 tcacagcaat caaaagcggt gcatctttgc caaacggaac cagtgcctca cgcgctgcgg
    38101 tcatcatacg cgccccacca gaggcatgaa cattcaccat ccacacgcct aagtcagctg
    38161 cagcagcgac agcgtgcgct gcagtgttag gaatatcgtg gaatttcagg tcaagaaaga
    38221 tatcaaaacc acgctgttga agttcgcgca caaactgtgg cccaaacaat gtaaacatct
    38281 ctttgccgac cttcagacga caatcgcgtg ggtcgatctt gtcgacaaag gacagcgcgt
    38341 catcacgatt atgataatca agggcaacaa ccacaggaga attcgtaaca gcgcgggaag
    38401 aagatgaagc agttaacgtc atgaccagac cttcttgatg atgggcaccg gaagtggcgc
    38461 gaaacaggta aacggcgagc attctacccg tggaaataaa aaattaacag ttgcgatttt
    38521 tcccctggca ggctttttct gctccgtcgt tgccggagca caatggaaca tcaataacta
    38581 gtatgttgta actaaagtcg gattttttta aaaattacag gccatcaaga ccgcgaatcg
    38641 gtttaatggt tgaccaggcc cgacaagacg gacaatgcca gtagagggtg tatgcggtaa
    38701 aaccacattt ctggcagcga taacgaggct tactacgtac cttttcgcca accatgtcac
    38761 gcagcaccat caggctctct ttggcacgcc cttcttccgc ttcatttaag tggtaatcca
    38821 ttaacttatg gaacacacgc atggtcggat gacgctgaag ctggcgcgta atatagacct
    38881 gtgcggcctc actaccgtcg cgcgcttcga tgatatcagc aagcatcaat tcagcatcgg
    38941 caccggtgtt ctcttccacc gcgcgttgca ggaattctgc ccattcggca gttttaccca
    39001 actgctgata gcaggtttgc aacatttcca gcgtttcgct gaccagttct ctgtcctggg
    39061 atatcacgcg ttgcaaactt tcgacggctt tggcgtattc tcctttcgcc ataaacacgc
    39121 gtcccatcat gatggatacg cgggcgctgt ttttatctgc cgccgcccct ttttttagca
    39181 aggtcatggc acgatcgaga tcgtcgctgg ccatatgctg cagggctaac tcacagtaga
    39241 aatgggcaat ttcgacgcgc tgtttatctt tacccagctt caccaggcgt tcggcaacat
    39301 caattgcttt ctgccactcg ctggtagcct ggtagatttg tagcaactgt tgcagcgcgc
    39361 caatgcggaa gtcagtttca tcggtcagct gattgaacat gtcttccgcg cggtcgtata
    39421 acccggcggc catgtaatca cgccccagtt gttgaatcgc caacagacgc tgttcatagg
    39481 tcagtgaggc gctttccatt agggtctgat ggatgcgaat agcgcgatca acttcgccac
    39541 gcgaacggaa caggtttccg agcgtaaggt gggcttcaac ggtgcctgta tcctctttaa
    39601 gcatatcgag aaacaggtct accgctttat cctgttgatt actaagcagg aagttaaccc
    39661 ccgctacgta atcacgcgac aagcggttgg cttcatcttg cttgttttgt tgcgcacttc
    39721 tgcggcccat ataccagcca taggcagcgg ctacaggcaa aagcagaaac aacaactcca
    39781 gcatagaaag ttattccttc gccgctgacg agtgcggcac tacagccacg tcagtcgcgg
    39841 gtgaaagctg gttttccagt cgctttattt tacgttcagc gcgcgccagg gaaacacgaa
    39901 ctcgcagcca gaacaggcca caaatcaacc aaccgatagc aacccccgca gcaaacaata
    39961 ccgccagcaa tgtggaaata cggtactccc cttgcgctaa cagataatta aacgtcacct
    40021 gttgatcgtt ctgcgcaccc aacgtgaccg aaatcacgaa gatcgctaac accagtaaga
    40081 aaatgagtaa atatttcaca ttacttcccg ttatgtggtt aaagcgaata aattatggtc
    40141 aacttaccgc aatagccctt taaagtacca ttttcgcgcc gtctgcgaaa tggcatccac
    40201 gcgcattgtt gatgatttaa gggctaaatt cggaaaatca accagcgtta actttcttgt
    40261 tctcgttgcg ctatttcgcg attttcttcc gcaggtggtg ttaatggccc acaaattcgt
    40321 tgcgcaagcc acgttgccac cgccaccagc gcccacgaaa tcaacgtagc tactaccaga
    40381 tcgcgtggcc aatgcatccc gagcagcagg cggcttccca tgactcccgt tgcccagacc
    40441 agcaagatag caatggttaa cgttcgccga cgcggccaca gcaaaccaac ggccagcagt
    40501 gcccaactgg cagcaaacat cgtgtgaccg gaaggaaagg caaaccccgt ctctttctgc
    40561 cagtgtgaac gcaaatattg tgggatattt ttctcttcag ccaactgttc tttcactaga
    40621 tttccgcgtt ctgctcgctt taaagtgtag aactcatcaa ccggaatatg atgtgttttt
    40681 tccagccaga taacaaaagg tcgtggttcc tggactttgt ctttgatcca ggatttaacg
    40741 ccttgtccca caatgattac ggccgccaga atggcaaata atacaaaggc agccttaatg
    40801 caaaaacgca gacaccagag aaaccagccg aataaaatca aatgtgtaat gacgccccag
    40861 ggctgggtga cagtttcagt aacccaaaaa gccgctttta gtagccaact ttgttctcca
    40921 ggttgccaac gccagccaga aatccatacg gctactggca tgacaagcaa tagtgcagct
    40981 cccactgcgg tacgtctggc aatcgaacgc atggcctctc ctttttgata agtcccacaa
    41041 tcataactga aaacgccagt tccaggaaaa attgacagat ttgtgccatt ccgtgaacga
    41101 tcgacgcgtc gtgattaggt gaaccccttc tcgttatggc aaaataagcc aatacagaac
    41161 cagcattatc tggagaattt catgcagctt aaacgtgtgg cagaagccaa actgccaacc
    41221 ccatggggcg atttcctgat ggtgggattt gaagaactgg caaccggaca cgatcatgtc
    41281 gcgctagtct atggcgatat ttccgggcat accccggtac ttgcgcgcgt ccattccgaa
    41341 tgtctgaccg gtgacgccct gttcagcttg cgctgcgatt gtggcttcca gctcgaagcg
    41401 gcattgacgc aaattgccga ggaaggccgt ggtattttgc tgtatcaccg tcaggaaggt
    41461 cgtaacattg gtctgctgaa taaaatccgc gcttacgcac tgcaggatca aggttacgat
    41521 accgtagagg ctaaccatca gttaggcttc gccgctgatg agcgcgactt cactctttgc
    41581 gctgatatgt tcaaactcct tggcgtcaat gaagtccgct tgttaaccaa taacccgaaa
    41641 aaagtcgaaa ttctgaccga agcagggatt aatattgttg aacgcgtacc attgattgta
    41701 ggtcgtaacc ccaataacga acattatctc gataccaaag ccgagaaaat gggtcatttg
    41761 ctgaacaaat aaccctctcg cattgtggaa ttaatttgct tgccggaagc aaaataaccg
    41821 gcaagcaaat atttgttact tcagcatatt acgaatgaca taatgcaaaa tgccgtcgtt
    41881 ctggtagtag gtcaactccg tcgcggtgtc gatacgacaa cggcagggta cgacctcctg
    41941 gctaccatcc gcgcgcgtaa gcgtcaccgg aaccgtcgca ccgggttgta gattttgcag
    42001 atcgccaata tcaatcttct cttccccggt tagtcctaac gttttacgcg ttacgccttg
    42061 cggaaattcc agcggcagga tgcccatgcc aattaaattc gaacggtgaa ttcgttcaaa
    42121 cgattcggca atcaccacac gaattccaag cagacgagga ccttttgccg cccagtcacg
    42181 gcttgagcct gaaccatact ctttcccggc aatcaccgcc agcggcgttt gttcctgctt
    42241 atagcgcatc gcagcatcat aaatagagac tacgtcgctg tcaggtaaat gccgcgtcat
    42301 ccccccttca acgccaggca ccatttcatt acggatgcga atattggcga aggtgccgcg
    42361 catcatcact tcatggttac cacgccgcga accgtaggag ttaaagtctt ttcgctcaac
    42421 accccgacct tgtagatatc gacccgctgg gctgtcgggc ttaatactgc ccgccggaga
    42481 gatatggtca gtggtgactg aatcccccag cattgcgagg atccgcgcac cgtgaatatc
    42541 ttccactggt gctggtgttg cctgcatttc atcaaagaaa ggcgataagc gaatataggt
    42601 tgagtcctcc tgccaaccgt aggtatcgga tcgtgtgacg ttaatttcct tccactctgc
    42661 tgtgccttca aaaacttctg cgtactcttt gcggaacatt tctgtggaga cttgttctac
    42721 cgcacgggca atttcttgtg ccgatggcca gatatctttc agataaaccg gatcgccttt
    42781 acgatcatga ccaataggct cagaagccag gttgatattc atatttcccg ccagcgcata
    42841 ggcaaccacc agcggcggcg aggccagcca gttggtttta accagcggat ggatacggcc
    42901 ttcaaagtta cggttgccgg acagcactgc accgacggtt aaatcgcctt ttttgattgc
    42961 cgtttcgata ggatcgggca gcggcccaga gttaccaata caggtagtac aaccgtatcc
    43021 cacaaggtta aaccccagtt cgtcgagaaa cggtgtcagt ttcgcttttg ccagatagtc
    43081 agaaacaact ttcgagcctg gtgccagcga cgctttgacc cacggttgcc gcttgaggcc
    43141 aagaattacg gcttttttcg ccagcaagcc tgcagccatc agcacacttg ggttagaggt
    43201 gttggtgcac gaggttatcg cagcaatgac cacagcgcca tcaggtaact gatactgatg
    43261 tccgttcata acataatcga ccggctggcg atctttatgc gtggcattca cttccagttc
    43321 gttactggcg gcaaatactt ttggtacatc gggcagtgca acgcgatcct gtgggcgttt
    43381 tggccctgcc aggctcgctt caacgtcatt catatccagt tctaacgtac tggtaaaaat
    43441 cggttcatcg cccgggttac gccacatgcc ctgcgctttg gcatattttt cgaccaactc
    43501 aacctgatct tcgctgcgcc cgcttaaacg catgtaatcg agggttacag catcgattgg
    43561 gaagaagcca caggtggcac catattctgg cgacatattg gcaatggtgg cgcgatccgc
    43621 cagcggtagt gaatccagac catcaccata aaattcgacg aatttctcca ccacgccatg
    43681 tttgcgcagc atttgggtaa cagtgagaac caggtctgtg gcggtaatac cttcacgtaa
    43741 tttaccggta agtttaaagc ccactacgtc cgggataagc atggaaaccg gctggcctaa
    43801 cattgcggct tctgcttcga tcccacccac gccccacccc agcacgccaa ggccgttgat
    43861 catagtggtg tgcgagtcag taccaacgag tgtatccgga taagcaatcc attcaccgtc
    43921 ctgcaattca ctccacactg ctttgccgag atattcgagg ttaacctgat ggcaaatgcc
    43981 tgtgcctggc ggcacgacgc taaaccgact gaacgcttgc tttccccatt tcaggaacac
    44041 ataacgttcg tggttgcgct ccatttccag gcgtacgttt tcttcaaatg cctcatcatc
    44101 accaaaacga tcgacggtca ccgagtggtc aatgaccagg tcgaccggtg agagcgggtt
    44161 aacctttgca gtatcgccgc cgaggcgttt aaccgcttcg cgcattgccg ccagatcaac
    44221 aacggcaggt acgccggtaa agtcctgcat caacaccctt gccgggcggt aggcaatttc
    44281 acggtcagca tgggcatttt tcagccatcc tgccagcgcg tggatatcct cttcggtaac
    44341 cgagttacca tcctgccagc gcagcaggtt ttcgagcaaa actttgagtg acttgggtag
    44401 acgggtgata tcgcccagtg atttagcagc aagcggcagg ctgtagtagt ggtaagtttt
    44461 atctttggcc tgcaacgtgt ccttactggc ttctcgtagg gttgacgaca tagctcctcc
    44521 ttaatgacag ggttgcgaag ccctgattct cttcagggtg aatattaaag ataacacaaa
    44581 catcgcgtaa cgatttgata acaacccaaa ttgataaaag aggagaaacc ttgtggcaaa
    44641 gcaaatgaca accccgccgc agcggggtca aggatcagtg agtaatgatc cagaggagtt
    44701 cggcccagaa gagcatcgac agagaaaacg cgcccatcca ggaccaatat ttcatatttg
    44761 tgttgatcat aggctacacc cttaacggtt attattttgt gttgagataa ctcaagctgt
    44821 tattgctgcg gtgtattaaa agccctttaa ttcattaaaa atgggtaata agaatgatga
    44881 taattattgt ttcggctgat catcttcagc aaacagttct ataaaggctt gctgctgcga
    44941 tgttagttgc cagtcgtctg gaataacgac ggaacattcc cgtttcgttt ctgtttcatt
    45001 gtcactatgc tggcataatg gtgacgactc cagttgctcc tgaccgacca tagttatccc
    45061 cacactttcc aaatcagcag tgcgacaacc acccagaaaa gtgcagaacc aagaaaaacc
    45121 gccagccagg ctttacgttt taattcagga tcgcggcggg gttcctgatt tccagaaggc
    45181 attgctaacc tcatataatc gccatcactt atcagcaaga cgttgaacga ttggtgtaat
    45241 taatcatacg atgagacaaa tcctaaagaa actttagt
//
LOCUS       DAJVRF010000013        43532 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJVRF010000013 DAJVRF010000000
VERSION     DAJVRF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.013"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..559)
                     /locus_tag="OQN13_000687"
     CDS             complement(<1..559)
                     /locus_tag="OQN13_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCS1614142.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
     gene            complement(762..998)
                     /gene="feoC"
                     /locus_tag="OQN13_000688"
     CDS             complement(762..998)
                     /gene="feoC"
                     /locus_tag="OQN13_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCS1614143.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(998..3319)
                     /gene="feoB"
                     /locus_tag="OQN13_000689"
     CDS             complement(998..3319)
                     /gene="feoB"
                     /locus_tag="OQN13_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCS1614144.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="OQN13_000690"
     CDS             complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="OQN13_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCS1614145.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4001..6322)
                     /locus_tag="OQN13_000691"
     CDS             complement(4001..6322)
                     /locus_tag="OQN13_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCS1614146.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6419..6895)
                     /gene="greB"
                     /locus_tag="OQN13_000692"
     CDS             complement(6419..6895)
                     /gene="greB"
                     /locus_tag="OQN13_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCS1614147.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            7123..7842
                     /gene="ompR"
                     /locus_tag="OQN13_000693"
     CDS             7123..7842
                     /gene="ompR"
                     /locus_tag="OQN13_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCS1614148.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7839..9191
                     /gene="envZ"
                     /locus_tag="OQN13_000694"
     CDS             7839..9191
                     /gene="envZ"
                     /locus_tag="OQN13_000694"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCS1614149.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="OQN13_000695"
     CDS             complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="OQN13_000695"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCS1614150.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11268..11582
                     /locus_tag="OQN13_000696"
     CDS             11268..11582
                     /locus_tag="OQN13_000696"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS1614151.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FG"
     gene            11595..12968
                     /locus_tag="OQN13_000697"
     CDS             11595..12968
                     /locus_tag="OQN13_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCS1614152.1"
                     /translation="MAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNG
                     LTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALVVILARTQSRLIDSIQ
                     KLFTLIATGLLPLVSLLTLMFIITLPFTDLSAISRHISAAGLLLTLAFLQLILMAIVR
                     DPQKASLPWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVL
                     LVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMA
                     RYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQ
                     EQVSEKVLAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQA
                     ERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLDLTVDGET
                     LEINLSK"
     gene            complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="OQN13_000698"
     CDS             complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="OQN13_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCS1614153.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="OQN13_000699"
     CDS             complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="OQN13_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCS1614154.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="OQN13_000700"
     CDS             complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="OQN13_000700"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCS1614155.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="OQN13_000701"
     CDS             complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="OQN13_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCS1614156.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17606..18166
                     /gene="nudE"
                     /locus_tag="OQN13_000702"
     CDS             17606..18166
                     /gene="nudE"
                     /locus_tag="OQN13_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCS1614157.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="OQN13_000703"
     CDS             complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="OQN13_000703"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCS1614158.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21003..21782
                     /gene="hofM"
                     /locus_tag="OQN13_000704"
     CDS             21003..21782
                     /gene="hofM"
                     /locus_tag="OQN13_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCS1614159.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21782..22321
                     /gene="hofN"
                     /locus_tag="OQN13_000705"
                     /pseudo
     CDS             21782..22321
                     /gene="hofN"
                     /locus_tag="OQN13_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
     gene            22305..22745
                     /gene="hofO"
                     /locus_tag="OQN13_000706"
     CDS             22305..22745
                     /gene="hofO"
                     /locus_tag="OQN13_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCS1614160.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22735..23139
                     /gene="hofP"
                     /locus_tag="OQN13_000707"
     CDS             22735..23139
                     /gene="hofP"
                     /locus_tag="OQN13_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCS1614161.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23051..24289
                     /gene="hofQ"
                     /locus_tag="OQN13_000708"
     CDS             23051..24289
                     /gene="hofQ"
                     /locus_tag="OQN13_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCS1614162.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24690..25211
                     /gene="aroK"
                     /locus_tag="OQN13_000709"
     CDS             24690..25211
                     /gene="aroK"
                     /locus_tag="OQN13_000709"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCS1614163.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25268..26356
                     /gene="aroB"
                     /locus_tag="OQN13_000710"
     CDS             25268..26356
                     /gene="aroB"
                     /locus_tag="OQN13_000710"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCS1614164.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26448..27734
                     /gene="damX"
                     /locus_tag="OQN13_000711"
     CDS             26448..27734
                     /gene="damX"
                     /locus_tag="OQN13_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCS1614165.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27841..28677
                     /gene="dam"
                     /locus_tag="OQN13_000712"
     CDS             27841..28677
                     /gene="dam"
                     /locus_tag="OQN13_000712"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS1614166.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28695..29372
                     /gene="rpe"
                     /locus_tag="OQN13_000713"
     CDS             28695..29372
                     /gene="rpe"
                     /locus_tag="OQN13_000713"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCS1614167.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29365..30123
                     /gene="gph"
                     /locus_tag="OQN13_000714"
     CDS             29365..30123
                     /gene="gph"
                     /locus_tag="OQN13_000714"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCS1614168.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30116..31120
                     /gene="trpS"
                     /locus_tag="OQN13_000715"
     CDS             30116..31120
                     /gene="trpS"
                     /locus_tag="OQN13_000715"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCS1614169.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31280..32185
                     /locus_tag="OQN13_000716"
     CDS             31280..32185
                     /locus_tag="OQN13_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614170.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32202..32564
                     /locus_tag="OQN13_000717"
     CDS             32202..32564
                     /locus_tag="OQN13_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCS1614171.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            32648..33811
                     /locus_tag="OQN13_000718"
                     /pseudo
     CDS             32648..33811
                     /locus_tag="OQN13_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            33811..35037
                     /locus_tag="OQN13_000719"
                     /pseudo
     CDS             33811..35037
                     /locus_tag="OQN13_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            35034..35912
                     /locus_tag="OQN13_000720"
     CDS             35034..35912
                     /locus_tag="OQN13_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCS1614172.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            35923..36276
                     /locus_tag="OQN13_000721"
     CDS             35923..36276
                     /locus_tag="OQN13_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCS1614173.1"
                     /translation="MKKIGVAGLQREQIKKTIEATATGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36288..37592
                     /locus_tag="OQN13_000722"
     CDS             36288..37592
                     /locus_tag="OQN13_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCS1614174.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37604..38689
                     /locus_tag="OQN13_000723"
     CDS             37604..38689
                     /locus_tag="OQN13_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCS1614175.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(38841..39572)
                     /gene="phoB"
                     /locus_tag="OQN13_000724"
     CDS             complement(38841..39572)
                     /gene="phoB"
                     /locus_tag="OQN13_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCS1614176.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="OQN13_000725"
     CDS             complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="OQN13_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCS1614177.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="OQN13_000726"
                     /pseudo
     CDS             complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="OQN13_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="OQN13_000727"
     CDS             complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="OQN13_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCS1614178.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTEC
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42378..>43532)
                     /gene="frlA"
                     /locus_tag="OQN13_000728"
     CDS             complement(42378..>43532)
                     /gene="frlA"
                     /locus_tag="OQN13_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCS1614179.1"
                     /translation="VYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDAPSL
                     SIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAKIIP
                     FTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMTGEI
                     KNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPALGST
                     AGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSIILQ
                     GALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGLMTT
                     LAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
BASE COUNT        10112 a        11068 c        11944 g        10408 t
ORIGIN      
        1 cgcgttgacg aatatgtttt tgaattaact ccagcagcgc catacgtcgg tgctgcatga
       61 tttcatcatc aggcatgacg gtgacatcaa tcagcggaaa tgcggaggca taaagctgcc
      121 ttgccagttt gggatcggcg aaacaatcca gccaacacag cgaataggga taggggcttt
      181 caataccgtg gtaaaacaac aatagcacca ccatcggcaa cgttttgtat ccagcatcaa
      241 ggtgattttg cattgcggca atagcgtaac gcatcatgcg aaatgcgatc agtttgtttg
      301 aggtgctttg atgttcaatc agacaataga tgtatcctgg tccttgttcc gttttcaccg
      361 accacagcac atcggaatag ctttcacgca gatcgtcatc aataaagctg ctcgactcca
      421 gcttgagggt tttcatatca caaagcgcgt gaatgggtgc cggtaaatga aacgcaagaa
      481 aatcacgagc cgtgtccggt tggcgtaaaa agagtttgaa cagcgcatcg tgtggggtgg
      541 aactctgctt tttgctcatg gcgttccttc acctgttgtt tgaatgccgc gactctacgc
      601 gccactccac acctgtgcat tagcgagttt tcatccctgc gagatgcttt caaaggaact
      661 tacgccgtgc tgcaagagtt gcatttttat ggaaagccgc acgcaaaaaa gcccggatgc
      721 agacgcatac cgggctttcc aaaacaggcg atggagtaag gttaacgcag cgcccaccac
      781 tcgcgcagac aggcttttcc ttccgggcag cttttacaac tgccagagag gcagccgtca
      841 ggttcttcct gaatccgcac ggctttgccc atactttcca gttgttgcag catggcgtta
      901 atcattggct gcggagtgtt caatgtctgg cttatctggg tcgcttccat acggccccgt
      961 aacgccagca aatcacgtac ctgaataagt gaagccatta atggcaatca ccggtggtgc
     1021 tggctgcgca gcaactgctt accgacttgc gggttgccag cagttcgata tccacccggc
     1081 tacgcgcgcg gcgcagcaga ccgataacta cgatgttaaa caggattacc gccagaatgc
     1141 acaccaggct gtaagttggg tgctgactgt agctggcgac ctgatagaac aatgttgcca
     1201 gtgagtaagc gatattcagc ccccacagga tggagaagcc catccagcca cggcttgatt
     1261 cacgggcgat ggctcccatc accgagatac atggtacata cagcaggacg aaaatcaggt
     1321 agctgtatgc ggcagctgcg ctaccgaatt tctgatccat cacgcccatc gccccggtac
     1381 ccatttcacc gtcgcctttg ctagcttcaa tggggttcat cagtacgcta aggctgaagg
     1441 tgtctttcag gctctgccag gtttcatcta ccgcactgaa cagctcttca ccgaggttga
     1501 attccgccgg attgaactct tcgtcctgaa tattttctgc ggtgtagagg gtgttgagcg
     1561 tacccaccac cacttctttt gccattgcgc ccgtaaacag gccaaccgtt gcctgccagt
     1621 tatcttcatg tacgccaatc ggcttgaaga caggggttat cacccggctg acggacgcca
     1681 gcgccgagtc gttgatgtta tcgacgattt tcccgctcaa cgagaagctg ttgaaagcgc
     1741 tcaggaaaat gctgacgatg ataatcactt taccagcacg cagaacgaag cccttcagac
     1801 gctgccaggt ctggataatc aggcttttaa cgtgtggtac atggtagacc ggcagctcca
     1861 tgacaaacgg cgtcgcttca ccgcgcatga tggtgtactt gagcatcagg ccagtcagca
     1921 ccgccatcac gataccgagc atatacagcg agaaaaccgc cagggcaccg ttctgcccga
     1981 agaaggcagc cgcgaatact gcgaagatag ccagacgcgc gccgcaggac ataaacggtg
     2041 ccatcatgat ggtcatcaga cgttcacgcg gtgcatcaag cgtacgtgcg cccattaccg
     2101 acggtacgtt acaaccgaaa ccgacgatca gcggcacaaa ggatttcccc ggcaagccca
     2161 gcgcctgcat caggcggtcc atcacaaacg ctgcacgcgc catatagccg gagtcctcaa
     2221 ggaaggagag gaacaggtac atcatgccga tctgcggcac cagtggcagc acggtgttaa
     2281 taccgccacc caggccctgg gcgaggaaga tagtcagcca gtccgggaag tggagcgtgt
     2341 agccaatcca ttgaatacca tgcacaaata gcgccacgga gccgacgtca aacagcggct
     2401 gtaacgcccc gccgatgttg atagccagca ggaacatcag gtacatcaca aagaggaaaa
     2461 tcggcagacc gaggaaacgg ttgagcacga ttttatctac cgcagtggtg aaacggctgg
     2521 gttctgccgt cagggtgttg cttaccacat cacagatggc agctatgcac tggtaacgcg
     2581 catcggcaat gtgcagcgcc ggatcatcca tttcattacg cagacgggca agggcggcat
     2641 ccagatgctg cgacgcttca ccggcgtagg cgcggctgta gatatcgcct tccagcattt
     2701 gcaggcccag ccagcgacgt tgtttcagcg ggatgtcgga aggcatcact tttgccagtg
     2761 aatctgcttc gttgagtagc ggctgtgcgt aatgcaccag ttccacattc tcgttagctt
     2821 tatagcgatc aatcgccagc ttgagcgctt caataccgcg accacgggtt gaaaccagcg
     2881 ggatcactgg gcagccaaga cgcgccgaca gagcatcaat ttcaatacga atattttgct
     2941 tctcggcaat gtcgagcatg ttcagtgcca cgatgcaggg aatgccgagt tccagcagtt
     3001 gtagcgtcag gtacaggtta cgctcaaggt tagacgcatc caccacgtta atcagcaggt
     3061 cggcgtcgcc actcaaaatg tagtgacagg cgatttgctc atcgagcgag gtctgcgatg
     3121 agatggtggt cagagaatag gtgccgggca ggtccaccag cgtgacctga tgatcggtgg
     3181 tggagaattg cccttcttta cgttcgacgg taacgccagc ccagttacct acacgctgac
     3241 gtgcgctagt gagctggtta aataaggttg tcttgccaga atttggatta ccaattaagc
     3301 caatggttaa ttttttcatt gttgttatca ccgtattaac aggaaaccgc ttccacttct
     3361 aataaggcca gatctttttt gcgtaatacc aggctcacac gacgggtttc gatatgaatg
     3421 gggtcgccga gtggagcgac gcgcaccaca ttaaaagagg agccaggtaa catgccaaga
     3481 gaaagcagtt tttggcgata tgccgggctg atttcacggg aaaagccagt gattttccac
     3541 gcagtatctg gagtgtattg cataggtgcc tacttgtttc tcattaactg gataactacc
     3601 tcatggggcg tttgacagcg acatgagacc agatgatttg ccaacaaagg agagattaaa
     3661 taatcaacaa cacaatgata atgagaatgg tttctatcag caatacttaa aatgtgtgcg
     3721 aatgttggtt tgaataataa tctgactcaa tgttgatgtg gctcaaggtt atatcgcagc
     3781 gaaaatataa ttcgaatagt gaattacccg gactggctaa tgtttaatta aggtttcagt
     3841 tgttaatgaa gtgataaata ttgccggaat atattatgag tttggttgtg gaataaaaag
     3901 gccggagata ttctccggca cttttatgtc aggcaaggcg ttcgttgcca gatgcggcgt
     3961 gaacgcctta tcgcgcaggt ttaggggcca gtccgggcgt ttaacgtttt ttgcccattg
     4021 ccgccgccag cgcatccatc atcgcgctat taccggcagg ctgcgcttca cgaccgcgtg
     4081 gtttggctgc cgggcggttg ttttgcgggc gttcattacc gccgccgcga cgggcgttgg
     4141 tttcgccagg ctgctcgtcc agacgcatgg tcagggcgat acgtttacgc tgaagatcca
     4201 cttccagcac tttcaccttc acaatgtcgc ccgctttcac cacggtatgc ggatcttcca
     4261 caaacttgtt cgacaatgaa gagatgtgaa ccaggccgtc ctgatgcacg ccaatatcga
     4321 caaacgcgcc aaagttggtg acgttggtga cagcgccttc gaggatcata cccggttgca
     4381 ggtcgttcat tgtctcgacg ccatcggcaa actgagcggt tttaaattcc ggacgcggat
     4441 cgcgacccgg tttttccagc tctttgatga tgtcagttac tgtcggcaca ccgaattttt
     4501 catcagtaaa gtcagacgct ttcaggttac gcagttcgct gctgttaccc atcagatctt
     4561 tcagtgcctg ctgtgttgct gccagaatgc gttccaccac cggataggct tccgggtgaa
     4621 cggtagaggc gtccagcggg ttatcaccgt ggttaatgcg caggaagccc gcgcactgct
     4681 cgaaggcttt cggccccaga cggctgactt tcagcagttg ctgacggttc ttgaactggc
     4741 cgttctcatc gcgccaggcc acgatgtttt gcgccatcat gcgcgtcagg cccgccacgc
     4801 gagttaatag cggaacagaa gcggtgttga gatcgacgcc aacggcgttt acgcagtctt
     4861 ctactactgc atccagtttg cgggccagtt gcgtctggct gacgtcatgc tgatactgac
     4921 ctacgccgat agatttcgga tcgattttca ccagctccgc cagcggatcc tgcaaacggc
     4981 gggcgataga taccgcacca cgcagcgaaa cgtcgagatc cgggaactcc tgcgctgcca
     5041 gctcggaagc tgagtaaacc gacgcgccag cttcgctgac gatcactttc tgtgcggtca
     5101 ctttcgggaa ctgcttctgc acgtcgagat agaaacgttc agtttcgcgg gaagctgtac
     5161 cgttgccgat cgccaccagt tcaacgttat gtttttcaca caaggcagca acggtcatcg
     5221 ctacttttgc ggcttgtccg gtgtgcgggt aaatggtatc ggtcgccacc agtttgccag
     5281 tggcatcgac cactgccact tttaccccgg tacgcagacc cggatcgagg cccatcgttg
     5341 cacgcagtcc ggcaggggcc gccatcagca gatcgtgcag gttacgggca aagacgttga
     5401 ttgcttcatc ttccgcgcgt tcgcgcacgg tacccatcag ttcggtttcc agatgcatca
     5461 gcaccttgat gcgccaggtc cagctcacta cgcctttgcg ccagctatcc gccggggcat
     5521 tgttcaggcg caggccaagg tgatccatga tgatttgctc gcaatagctc tctttgggcg
     5581 gctcatcgaa ctgcggatcg gcattcagcg aaagctggag tacgccttcg ttacgcccac
     5641 ggaacatcgc cagcgcgcgg tgagaaggca ccgtggacaa cggttcgtga tgatcgaaat
     5701 agtcgcggaa tttcgcccct tcctcttctt taccgctcac caccgtagaa accaaatgcg
     5761 cgttcttcca cagataatca cgcactttcg ccagcagcgc ggcatcttcg gcaaaccgtt
     5821 ccatcaggat atagcgcgcg ccgtccagcg cggctttggt atctgccacg cctttatcgg
     5881 catcaatata ttgcgcagcg gcgacttctg gtgtgtgtga cggatcgctc cacagcaggt
     5941 cagccaacgg ctcaagccct gcttcaatgg cgatttgccc gcgggtgcgg cgtttaggtt
     6001 tgtagggcag gtagaggtct tcgagttcgg ttttgcttag ggtggcgttg atggccttcg
     6061 ccagatcatc ggtgagtttg ccttgctcgg aaatggactt gaggatcgcc tgacgtctct
     6121 cttccagctc gcgcagatag ctcagacgcg tttccagatt acgcagctgc gtgtcatcca
     6181 gaccgccggt gatttcctta cgataacgtg cgataaacgg cacggtattc ccttcgtcaa
     6241 gcaggcgaac ggcagcgtca acctgttccg ggcgcgcctg aatttcaccc gcaataatgc
     6301 ggcagaacga atcattcatc atggtttgag ttcatctttt cggatcaaaa atcaggggat
     6361 agttatacgg actggctggc aaaaatgcca gccatcggca ggaggttaag actcttcatt
     6421 acggtttcac gtattcgata gcattaacat accagctcgc ttccccggca ggggtattca
     6481 ccaccgccag atcgccgact tcttttttca gcaatgcgcg ggccatcggg gaatcgatag
     6541 agatgtaatc tttacggcca aaaatttcat cgtagccgac aatacggaaa cggtgagtca
     6601 cgccatcgtc gttttcaatc tccacccacg cgccaaaaaa gactttgcct tcctgctgag
     6661 gggagtaatt gacgattttg agattttcca tgcatttagt gagatagcgc acgcgacggt
     6721 cgatttcacg cagacgcttt ttattatact gatagtcagc attttcgctg cggtcgccca
     6781 gacttgcggc ccaggtcacc ttttttgtga cctccgggcg ttcttcacgc cagagataat
     6841 taagctcttg tttgagtttt tcataccctt cccgggtaac caggggcgtt ttcatctcgt
     6901 tgattccctt tgtctgtttg ataatgcgca cattgggtat aacgtgatca tatcaacaga
     6961 atcaataatg tttcgccgaa taaattgtat acttaagctg ctgtttaata tgctttgtaa
     7021 caatttaggc tgaaattcat accagattta gctggtgacg aacgtgagct tttttaagaa
     7081 tacacgctta caaattgttg cgaacctttg ggagtacaaa caatgcaaga gaactacaag
     7141 attctggtgg tcgatgacga catgcgcctg cgtgcgctgc tggaacgtta tctcaccgaa
     7201 caaggcttcc aggttcgaag cgtcgctaat gcagaacaga tggatcgcct gctgactcgt
     7261 gaatctttcc atcttatggt actggattta atgttacctg gtgaagatgg cttgtcgatt
     7321 tgccgacgtc ttcgtagtca gagcaacccg atgccgatca ttatggtgac ggcgaaaggg
     7381 gaagaagtgg accgtatcgt aggcctggag attggcgctg acgactacat tccaaaaccg
     7441 tttaacccgc gtgaactgct ggcccgtatt cgtgcggtgc tgcgtcgtca ggcgaacgaa
     7501 ctgccaggcg caccgtcaca ggaagaggcg gtaattgctt tcggtaagtt caaacttaac
     7561 ctcggtacgc gcgaaatgtt ccgcgaagat gagccgatgc cgctcaccag cggtgagttt
     7621 gcggtactga aggcactggt cagccatccg cgtgagccgc tctcccgcga taagctgatg
     7681 aaccttgccc gtggtcgtga atattccgca atggaacgct ccatcgacgt gcagatttcg
     7741 cgtctgcgcc gcatggtaga agaagatcca gcgcatccgc gttacattca gaccgtttgg
     7801 ggcctgggct acgtctttgt accggacggc tctaaagcat gaggcgattg cgcttctcgc
     7861 cacgaagttc atttgcccgt acgttattgc tcatcgtcac cttgctgttc gccagcctgg
     7921 tgacgactta tctggtggtg ctgaacttcg cgattttgcc gagcctccag cagtttaata
     7981 aagtcctcgc gtacgaagtg cgtatgttga tgaccgacaa actgcaactg gaggacggca
     8041 cgcagttggt tgtgcctccc gctttccgtc gggagatcta ccgtgagctg gggatctctc
     8101 tctactccaa cgaggctgcc gaagaggcag gtctgcgttg ggcgcaacac tatgaattct
     8161 taagccatca gatggcacag caactgggcg gcccgacgga agtgcgcgtt gaggtcaaca
     8221 aaagttcgcc tgtcgtctgg ctgaaaacct ggctgtcgcc caatatctgg gtacgcgtgc
     8281 cgctgaccga aattcatcag ggcgatttct ctccgctgtt ccgctatacg ctggcgatta
     8341 tgctattggc gataggcggg gcgtggctgt ttattcgtat ccagaaccga ccgttggtcg
     8401 atctcgaaca tgctgctttg caggtcggta aagggattat tccgccgccg ctgcgtgagt
     8461 atggcgcgtc cgaggtgcgt tccgttaccc gcgcctttaa ccatatggcg gctggtgtta
     8521 agcaactggc ggatgaccgc acgctgctga tggcgggggt aagtcacgac ttgcgcacgc
     8581 cgctgacgcg tattcgcctg gcgactgaga tgatgagcga gcaggatggc tatctggcag
     8641 aatcgatcaa taaagatatc gaagagtgca acgccatcat tgagcagttt atcgactacc
     8701 tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct taatgcagtg ctcggtgagg
     8761 tgattgctgc cgaaagtggc tatgagcggg aaattgaaac cgcgctttac cccggcagca
     8821 ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg
     8881 ccgctcgtta tggcaatggc tggatcaaag tcagcagcgg aacggagccg aatcgcgcct
     8941 ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc
     9001 agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg cacgggatta gggctggcaa
     9061 ttgtgcagcg tatcgtggat aaccataacg ggatgctgga gcttggcacc agcgagcggg
     9121 gcgggctttc cattcgcgcc tggctgccag tgccggtaac gcgggcgcag ggcacgacaa
     9181 aagaagggta aataaacggg aggcgaaggt gcctcccgtt ttgctttcta taagatactg
     9241 gatagatatt ctccagcttc aaatcattac agtttcggac cagccgctac cagcgcggca
     9301 cccgcagggg tgtcggtgta tttatcgaag ttgtcgataa acagtttcgc cagggtttcg
     9361 gctttttcct gccactgttc cggagaagcg taggtgttac gcggatcgag aatcttcgtg
     9421 tctacgcccg gcagttcggt tgggatcgcc aggttaaaca tcggcagagt gaaggtttct
     9481 gcattatcca gcgaaccgtt gaggatggcg tcgataatgg cgcgggtatc tttaatcgag
     9541 atacgtttgc cagtgccgtt ccagccagtg ttaaccagat aagcctgcgc gcccgccgcc
     9601 tgcatacgtt tcaccagcac ttctgcgtac tgagtcgggt gcagcgacag gaacgccgcg
     9661 ccgaagcaag cggagaaggt tggcgtcggt tcggtgatgc cacgctcagt accggccagt
     9721 ttggcggtga agccagagag gaagtgatac tgggtttgat cggcagtcag gcgagaaacc
     9781 ggcggcaaca cgccgaaagc atcagcagtc aggaagataa ccttagtcgc gtggcccgct
     9841 ttggaaaccg gcttaacgat gttatcgatg tgatagattg gataagaaac gcgggtgttc
     9901 tcggtttttg aaccatcatc aaagtcgata gtgccatctt cacgcacggt gacgttttcc
     9961 agcagcgcat cacgacggat agcgttgtag atttcaggtt ccgcttcttt cgacagcttg
    10021 atagtttttg cgtagcagcc gccttcgaaa ttaaacacgc cgtcatcgtc ccagccgtgt
    10081 tcgtcatcgc caatcaggcg gcgtttcggg tcggtggaaa gggtggtttt accggtgccg
    10141 gaaaggccga agaacaccgc aacatcgcct ttctcgccaa cgttggcgga gcagtgcata
    10201 gaagcgatac ctttcagcgg cagcaggtag ttcatcatcg agaacatccc tttcttcatt
    10261 tcgccgccgt accaggtgcc gccaatcagc tgcatgcgct cggtcaggtt aaacgccacg
    10321 aagttttcgg agttcagacc ctgttctttc cactgcgggt tagtgcactt cgcgccgttc
    10381 ataacgataa agtctggttt gaaacctgcc agctcttcat cgctcgggcg aataaacatg
    10441 tttttgacaa aatgcgcctg ccaggccact tcggtgatga aacggacgga aagacgagta
    10501 tccgggttcg caccacagaa agcgtcgaca acgaacagac gtttgccgga aagctgcctg
    10561 gtcaccaggc ctttcagatg ctgccaggtt tccggagaga gaggtttgtt gtcgttctta
    10621 cctttgcctt tgtctgccca ccagaaagta tcgcgagtgg tatcgtcacg gacgatatac
    10681 ttatcttttg gtgaacgacc ggtgaagatc ccggtatcga cggcaacggc acccagatta
    10741 gttaacaccc cgcgctcata acctgtcagg ctcggatcga gctcttcctg atacagcagg
    10801 tcgtagcttg ggttgtaaac gatatcatgt acgtcactga taccataagc ctcgagttct
    10861 tgcggggtca aaccattgtt aacgcgcatt tcactgctcc ttagccaata tgtattgcct
    10921 gaatagtaaa gtctttttgg gggtgttaac cgcgacaagg ctcatagatt tacgtatctg
    10981 gagaaattca tatttgacaa aaaactccgt gattcctgtc acgaaacggt tgctattatc
    11041 gcagaaatag cattttaggt gtggaaaatg ttctcaaaag gttaaatctt ggtttatatg
    11101 gcgggaatat tgtgagtgga atcgcattcc tgaatgaaat tgatgcaaat tcaaccggta
    11161 aagtatcgat tcaccacgct aacttttttt gattattttc tgaaaaaagc aaaatactac
    11221 tgcccggatg cgataattaa cccacctttt gattctggat aaccaacatg gataacgttg
    11281 aactttcacc ggcaacacgc tgggggatga ttgctaccgg attacttcag gggctggttt
    11341 gctacctgct tatcgcctgg cttgccggaa aaaatcacag ttggattgtt tatggcgtgc
    11401 ctgcgaccgt cgcgttttca tccgttttac tcttctccgt gatctctttt aaacagaagc
    11461 gcctttgggg atggctggcg ctggtgttta ttgccacgac agggatgagc ggttggctga
    11521 agtggcaaac tgatggcatg aacccctgga gagctgaaaa ggcgatttgg gattttggct
    11581 gatatctgct gttgatggca atgttgttgc taccgtggat acaacaaagc cttcgtatcc
    11641 gcaatggcag tagccgctat agctactttt accagtcagt atggcataac gtacttatat
    11701 tactggtgat ttttctctcc aatggcctga cgtggctggt gcttttactg tggggtgagt
    11761 tgtttaaact tgttggcatc acgtttttca atacgctctt ttttgcaacc gactggttta
    11821 tgtatcttac atttggtctg gttaccgcac tggtggtgat cctcgcgcgt acacagtcac
    11881 gtttaatcga ctctattcaa aagttgttca cgctaatcgc cacggggttg ctgccgttag
    11941 tatcattgct aaccctgatg tttatcatca ccctgccgtt tacggacctg agcgcgattt
    12001 ctcgccacat ctccgccgcc gggttgctgt taacgctggc ctttttgcaa ttgatcttaa
    12061 tggctattgt ccgcgatccg caaaaagcgt cacttccctg gacggggccg ttgcgttgcc
    12121 tgattaaaac cgctttgctg gttgctccgc tgtatgtgtt cgtcgccgcc tgggcgttat
    12181 ggctgcgggt cgctcagtac ggctggactg tcgaccgctt gcagggcgcg ctggcggtgc
    12241 tggtgttact ggtgtggtcg ctggggtatt ttgtcagtat cgtctggcgt aatgggcaaa
    12301 atccccttgt tcttcagggc aaagtgaacc ttgcggtttc gttattggtg ttggtgatac
    12361 tggtgcttct taattcgccg gtgctggaca gtatgcgcat tagcgtgaac agtcatatgg
    12421 cgcgttatca gagcggcaaa aacacgccag accaggtaac tatctacatg ctcgaacaga
    12481 gcggtcgcta tggacgtgcg gcgcttgagt cgctgaaaag cgatgccggg tttatgaaag
    12541 acccgaaacg cgcgcgggat ctgctgatgg cgttagatgg agagcaacat cttcaggaac
    12601 aggtatcgga aaaagtatta gccgaaaatg tgttaattgc tcctggttct gttaaaccgg
    12661 atgcgacatt ctggtcggcc ttaatccagg atcgctataa cgtgatgacc tgtattgaaa
    12721 aagacgcctg cgtcctggtc gagcaagatc tgaatagtga tggtcaggcg gagcggatcc
    12781 tgtttgcttt taatgatgac agagtcattg tctatggctt tgactcagac agaaaagaat
    12841 gggacgcgct tgatatgagt ttacttccga acgaaataac gaaagaaaaa ttactcacag
    12901 ctgcgaagga tggaaaactg gatctgacgg tagatggtga aacgctggaa ataaacttaa
    12961 gtaagtgaca actcttttaa cggatacgac gctgtatctg accaacaata atttgcgatt
    13021 tatttcacag gcctggtaag cgcagcacat caggccatta cgtcgtaaaa tccggcagag
    13081 ccctctctgc cggacatact cattaatgaa cttgcggatc tgccggagac gcgttgttgc
    13141 ggatttcagc aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca
    13201 tgtcaatttc gccatcttcc gccaggatgc tatcgacttc ttcatcaggc agcgttttca
    13261 gcgcgtcggc gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt
    13321 aaaccgtcac ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca
    13381 gcagttcttc ggttttgatg gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgct
    13441 gggcattttg cgcaggcatt acctgcaaca acataccgcc tgcagccggt ttgccgtcta
    13501 cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt
    13561 ccaggcaggc cgccagggta tcaccttcca gaccaaccac gccctgatag cgttcgcctt
    13621 cgctcggggt aatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat
    13681 tttctggaat ttcgccctgc acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt
    13741 taataaccgc cagattcatc ggaccgtcgc cttgcagctg tacggtgata tcaccatcaa
    13801 acttcagcgt agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa
    13861 cgggctgcgg ataatcgtga ttctcaagga tctgttgcag ggtttccgaa acggttacca
    13921 gttcgccgcg cacggcaaag ttttcaaaca gatagcgatg taattggtca tgttgcggca
    13981 taatcatctc tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt
    14041 cgcggcgctc ttttttgtcc gggcgtcggt ccgggtgcgg catggttaag gcattaagtt
    14101 tacgtgccag cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca
    14161 aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt
    14221 cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc gacgattttg ctcggcttgc
    14281 tgcgctgccc gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg
    14341 ttttataaaa acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggttttt
    14401 ctttcatggc gtctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac
    14461 ggatggcgtt gatactgttt ctcggcaatc ccggaatcag gattagtcac gccgaggcag
    14521 taacgaatac caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc
    14581 gttctttcag ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct
    14641 ttcggataac caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagaccggta
    14701 tgctcaagtt ttaccgccag gttgtgcgga tgcgcattgg tgagcaaaat tcgctgctta
    14761 ccgctggctt tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcacgcggt
    14821 cccatctcgg tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga
    14881 cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct
    14941 tcctgtggcg taaccccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa
    15001 tagttatcga aggcgaggtc gagcaacgtg ccgtccatat ccagcagaac ggtatctacg
    15061 tcctgccagg caatgttgat atgcatgagg gaaatctcca gagtgaagca atttgcgcga
    15121 cagggtagca taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat
    15181 cagttgcggg ttcaggcagc tttcataata ggcctgaatc ttcatcatgc gagtgcgatg
    15241 acgctggtaa cgacgccagg cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag
    15301 tagcagcaat gtggtgctga gatagcgcca caggccggaa cgatccggga ttggatgaag
    15361 gccaatatgc tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cctcggcgtt
    15421 aaacggcgta tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg
    15481 cgggtagtcg taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc
    15541 gctgacaatc aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt
    15601 ttcatgcgtg ataaacggcg cggtggaggt tgccaccagg ttatccagcg attccgcact
    15661 caccgggcgt aataacacat tcacgccatc gagcttcccg gcgttggcgc gttttaccag
    15721 cgcgtcccag tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc
    15781 atctttggca gaacataaat ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa
    15841 taccatgccg gatttttgaa ttgctgaacg taatgaggca ctgacgcgag atccatcttc
    15901 cggtttaggg tgcagctggc gattaactgc ttcagtcaat gccgtcgctt tgttgaccag
    15961 ttcagattct ggtaatggca atgagcgggc gtcgttccag atgatctgcg agcagtcaaa
    16021 cggtaaaaaa ggtgaattgg ttttcgcgct ccaggttccg gaagtgcgaa tattacacat
    16081 tcccgtaccg ctgatacgca atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt
    16141 tacgctggtg gcttcaatgg tctgtgcgcc tttcatccat gagagagtga atttcagcgg
    16201 catatccagc gggatccaga ataacagcat aaacagcacc agcagcgagc ccgcagcgat
    16261 aatcgtactg cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag
    16321 atatcgcccc tgacgcacta cgtggcggtc gagatagata tcaatatcgg tttgttgacc
    16381 gagatcctga gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag
    16441 tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt
    16501 accgcgcaga caatgaattt cccgcaggga ggattttgcc gggggcgcga atagccccca
    16561 cagacctgcg cccagcagca gtaatgcgcc gcctgccagc cacggaacaa atacatccgg
    16621 ggtaatcagg cagaaaaaga acatcaggaa ggaggcgacg atcagcaacg cttcacgcag
    16681 cccgcgcgga cgactcagcg catattcttc atgcgtttct ttgcgaatat tgagtagctc
    16741 gatttgctca ctctcttccc cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc
    16801 atagccgcgc gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccag
    16861 cggcagcgaa tcggtataaa tcagttcaac ggtattctca tcgttgatgt attgttccca
    16921 gaaagggggc aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc
    16981 ggtagagatg ccgtaacgcg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc
    17041 attcagcgcc agagagatcg gtgccgcgct agctcccgtt gggccaggca cctgtaatac
    17101 ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt tcttcaggtg tgagtttacg
    17161 cgtttgcgca tccgcgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt
    17221 tatcagccat cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt
    17281 gctcatgctt tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cagacacaat
    17341 acatgattct gcggttggct atcggcaagc gtggagttta acttgagcat cattaagcac
    17401 atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg ggatatcagc aaattcctgg
    17461 agttatttca acctattgac ttttaatttg atttgaggac gattatttat taagttacat
    17521 aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg
    17581 ctgatttcac agcatagatc ccacgatgag caaatcatta caaaaaccca ccattctgaa
    17641 tgttgaaact gtagcccatt cccgactgtt taccgtcgag agcgtggatc tggagttcag
    17701 caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat
    17761 tgtgccgatt gtggacgatc acctgatcct gatccgcgaa tacgcagtgg gaactgaatc
    17821 ctacgaatta ggtttttcga aaggattaat tgatccgggt gaaagcgtct acgaagccgc
    17881 taaccgcgag ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa
    17941 gctcagcatg gcaccgtctt acttttccag caaaatgaat atcgtggtcg cgcaagatct
    18001 ctacccggaa tcgctggaag gcgatgagcc agagccgcta ccacaggtgc gctggccgct
    18061 ggcgcatatg atggatttgc tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc
    18121 gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg
    18181 cctgatgcac aacgattatc aggcttatgg ggttcctgca atatattgat ttttcacgat
    18241 tttgttagcc ggataacgcg ttcacccgca tccggcataa acaaatgcac tttgtcagcc
    18301 aactgaaaag gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct caccattatc
    18361 gataatggtt gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa
    18421 atactcttcg cggctgttgc caccattagc taactgcccg gtgctgcgat cgatattcac
    18481 cgtcacaata cccggaggcg gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt
    18541 catataagcg tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg
    18601 atctttaatc gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc
    18661 aatccagacc gaggtcacaa cgcccggacc gtaacccgag aaccacgcat ctttcgaact
    18721 gttagtggta ccggttttcc cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca
    18781 gccggtaccc tgccagcctg gttcaccaaa gatattggtg ttcaaagcac tcttaatcag
    18841 gaatgccagc ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac
    18901 taacgcctga tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg
    18961 ggagatagcg acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc
    19021 gtaaatcacc ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaatcac
    19081 gccgccctga tcgttttcaa ttttgctgat aaaccacggg tccaccagga agccgccgtt
    19141 cgccatgacc gcgtagccgc gcgccacctg cattggggtg aaggacgctg aacccagcgc
    19201 cagcgattcg gtgtggacaa tgttttgcgc cgggaagccg aagcgttgca gatattctgc
    19261 agcgtagtcg acgcccatcg cccgcattgc acgtaccatc accacgtttt tcgactgacc
    19321 cagcccctga cgtaagcgaa ttggaccagc atactgcggt ggtgagttct tcggctgcca
    19381 gtcagaaccg acacctgcat cccagcgaga aattggcaca tcgttcaaca tacttgccag
    19441 cgtcagacct ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg
    19501 acgcagtgcc tgggtggcgc ggttaaactt gctctgattg aaatcaaagc caccgaccag
    19561 cgccataacg gcaccatttt gcggattgat cgacaccagc gccgagttca cttccggcac
    19621 ttgtgccagc caccatgcat cgccaacctg acgaacccag atttgctgac ccgtttgcag
    19681 aacatcggtc actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc
    19741 ccagcgaacg ccgtccatac tcaatgcgac agtcgacccg tccgccagca ttgccgtcgc
    19801 ttcctgagga ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc
    19861 cttcagcgta tcggtaatct tgttgttatc ccacgccgac tcgcccactt tccacagcac
    19921 atttgccggg ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc
    19981 ctgctgtgcg gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc
    20041 ataggcactt tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtacgg
    20101 cgcagagaaa gcaatctccg gcgcgtgata gttagcgtta atcgcctcag tgcgtgtctg
    20161 atcgaactgt tgttgggtga tatacccttc atccagcatc cgcgacagta cgacgttacg
    20221 ccgcgcgacg gcacgatcca ttgagtagag cggattaaag gtggaaggcg ctttcggcag
    20281 cccggctatc acagccattt cgttcagcgt cagttggtcg accgttttac cgaaatagac
    20341 ttgtgccgca gcaccgacac cataggcgcg gtaaccgagg taaatcttgt tcagataaag
    20401 ctcgaggatc tcgtctttcg tcagcaactg ttcaatgcga atcgcgagga agacttcctt
    20461 aatcttacgc atcagcgtgc gttctggact gaggaagaag tttctcgcca gctgctgggt
    20521 aatggtactc gccccttgtg acgcgtggcc ggagaacagc gccacacttg ccgcacggaa
    20581 gatccccacc gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa
    20641 ggctttcacc atctccggcg ggatttgatc caacgtaacc ggaatacgac gtttctcacc
    20701 gtattgagca atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac
    20761 atcttttaat gtcgccacat ccggtagttg tggctcgatg tagcggtata ggccataaat
    20821 cgagcctgct cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt
    20881 cactggaaat ttcccattta gtttcatttg ggcagtttat aaacaaacgt gcggtagtat
    20941 aaaggcaagc cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca
    21001 caatggcatt taagatctgg caaattggtt tgcatttaca acagcaagaa gcagtagcgg
    21061 ttgcgatcgt gcggggcgca aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg
    21121 agaacgacat tatcaaagat gggcggattg ttgatgcgca gcggctggct aaaacgttgt
    21181 taccgtggag tcgcgaactg ccgcagcgtc atcacattat gttggcgttt cccgccagtc
    21241 gcacattaca gcggtcattt ccgcgcccgt cgatgtccct cggtgagcgg gagcaaacgg
    21301 cctggctgtc agggacgatg gcccgcgagc tggatatgga tccggactcc ctgcgtttcg
    21361 attacagcga agactcactc agtcccgcct ataacgtgac tgccgcgcaa agcaaagagc
    21421 tggcaacgct actcactctg gcagaaaggt tgcgtgttca tgtgagcgcg atcaccccgg
    21481 atgccagtgc attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct
    21541 ggcgtgataa cgaacagtgg ctgtgggcga cacgctgtcg ctgggggcgc aaactggcgg
    21601 tggggatgac tagcgcgaag gagctggcgg cagcgttatc cgttgatccc gaaagcgtcg
    21661 cgatatgtgg cgaaggcgga tttgatccct gggaggccgt ttctgttcgt cagccgccgc
    21721 taccgccgcc aggtggagac tttgccatcg cgctggggct ggcgcttggg aaggcgtact
    21781 gatgaacccg ccaattaatt ttttgccctg gcgacagcaa cgccggaccg cttttcttcg
    21841 tttctggttg ctgatgttcg ttgcgcctct gctgctggcc gtcgggataa cgctaatact
    21901 gcgtctgaca gacaacgccg aagcacgcgt aaacaccgtt ttgcttcagg cggaacaaca
    21961 actcgcccgc agcttacaga taacgaaacc acgtttgctg gagcgccaac aaattcgtga
    22021 gcagcgtttg caacggcagc gccagcgaca atttacccgc gactggcaat ctgcgctgga
    22081 agcactggcg gcgcttttac ccgagcacgc ctagctgaca acgataagct ggcagcaggg
    22141 aacgctggag atcaaggggc tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc
    22201 actccgccag gatgcgtctt ttcatctcaa tcagcgggga gccacgcagc aggatgcgca
    22261 gggacgctgg caatttgagt atcagttaac aaggaaggtt agcgatgaac atgttctttg
    22321 actggtggtt cgccacatca ccccgcctcc gccagttttg ctgggcattc tggttgctga
    22381 tgttagttac gctcattttt ctgtcatcga cacaccatga agagcgcgac gcattaattc
    22441 gactacgggc aagtcatcac cagcagtggg ccacactgta tcgcctggtt gacaccactc
    22501 ccttcagcga agaaaaaacg ctgccctttt cgccactgga ttttcagttg cccggcgcgc
    22561 aactggtttc ctggcatcca tccgcgcagg gaggcgagtt ggcgttgaaa acgctgtggg
    22621 aagcagtgcc gtcggcattt acacggctgg cagagcgcaa cgtcagcgtg agccgttttt
    22681 cattaagcgt ggaaggtgat gatcttttgt tcacgctaca actggagacg ccgcatgagg
    22741 gttaaacgct ggttgttggc aggtattgta ttgtgccttt taaccggtat gcgtgaccct
    22801 tttaaaccgc cggaagatct atgccggatt agcgaactta gccagtggcg ctatcagggg
    22861 atggtagggc gaggcgagcg catcatcggt gtaataaaag acgggcaaaa gaaatggcga
    22921 cgggtgcagc aaaacgatgt gctggaaaac ggctggacaa ttttacagct gacgccagac
    22981 gtactaacgc tgggtaccgg gacaaactgc gaaccgccac aatggttgtg gcaacggcaa
    23041 ggagatacaa atgaagcaat ggatagccgc actactgttg atgctgatac ccggcgtaca
    23101 ggcggcaaag ccgcaaaaag tgacgctgat ggtggatgac gttccggtag ctcaggtgtt
    23161 gcaggcgctg gctgaacagg agaagttgaa cctggtcgtg tcgccagacg tcagcggtac
    23221 ggtgtcgtta catctaacag atgttccctg gaagcaggca ctacaaactg tagtgaaaag
    23281 cgccggactg ataacgcggc aggaaggcaa cattctctca gtgcattcca ttgcctggca
    23341 gaataacaat atcgcccgcc aggaggcgga gcaggcgcgg gcgcaggcaa atctgccgct
    23401 ggaaaatcgc agtataaccc tgcaatacgc cgacgcggga gaactggcga aagcggggga
    23461 gaagctactg agtgccaaag ggagtatgac cgtcgataaa cgcaccaatc gccttttgct
    23521 acgagataac aaaacggcgt taagcgcgct tgaacagtgg gtagcgcaaa tggatctgcc
    23581 ggtcgggcag gttgagctgt cggcgcatat tgtcaccatt aatgaaaaaa gtttgcgtga
    23641 gttaggcgtg aaatggacgc tggccgatgc gcaacacgct ggtggcgttg ggcaagtcac
    23701 cacgcttggt agcgacctct ccgtagcgac ggcgacaacg catgtcggtt ttaacattgg
    23761 gcgcatcaac ggacgcttgc tggatcttga gctttccgcg ctcgaacaaa aacagcagct
    23821 ggatattatc gccagtccgc gtctgctggc ctcacatctt cagcctgcca gcattaaaca
    23881 ggggagcgaa attccatatc aggtttccag cggggaaagt ggcgcgacgt cggtggaatt
    23941 taaagaggcc gtcctgggga tggaagttac gcccacagtg ttacaaaaag gtcgcatccg
    24001 gctgaaatta cacatcagcc agaacgttcc ggggcaggtg ctacagcagg ccgatggcga
    24061 agtgctggcg attgataagc aggagatcga aacgcaggtc gaggtcaaaa gcggagaaac
    24121 gttggcgctg ggcggcattt ttacccgtaa aaataaatcg ggtcaggata gcgtaccgtt
    24181 gcttggcgac attccctggt tcgggcaatt atttcgtcat gacggaaaag aagatgaacg
    24241 acgcgagtta gtggtgttta tcacgccacg actggtttcc agtgagtaaa cagccgtaaa
    24301 agcggtaatg tttttacgct gaacgtgttt catctatttg acgcgcgcag gtatttagca
    24361 tacaaggagt accgatttga gagttggtgc tcttcgctgc ctgcgttcca tgatgatgat
    24421 ttatcattca ggcggcattt tgctgtcttt tttacgctaa tcttacccgg tgatttatcg
    24481 ccagagcggt ggtagcaagg cagcgcgctt gcagcgacca gatatgcaga gggatgggtg
    24541 atttattcag ttgccaaacc cgctggagta ttgagataat tttcagtctg actctcgcaa
    24601 tatcttatga ggtttcagtt catgtcctgc ggcgctctct gagcgaagcg ggtttatcat
    24661 taacgaatag tcttagtagt accgaaaaaa tggcagagaa acgcaatatc tttctggttg
    24721 ggcctatggg tgccggaaaa agcactattg ggcgccagtt agctcaacaa ctcaatatgg
    24781 aattttacga ttccgatcaa gagattgaga aacgaaccgg agctgatgtg ggctgggttt
    24841 tcgatttaga aggcgaagaa ggcttccgcg atcgcgaaga aaaggtcatc aatgagttga
    24901 ccgagaaaca gggtattgtg ctggctactg gcggtggctc tgtgaaatcc cgtgaaacgc
    24961 gtaaccgtct ttccgctcgt ggcgttgtcg tttatcttga aacgaccatc gaaaagcaac
    25021 ttgcacgcac gcagcgtgat aaaaaacgcc cgttgctgca cgttgaaaca ccgccacgtg
    25081 aagttctgga agcgttggcc aatgaacgca atccgctgta tgaagagatt gccgacgtga
    25141 ccattcgtac tgatgatcaa agcgctaaag tggttgcaaa ccagattatt cacatgctgg
    25201 aaagcaacta attctggctt tatatacact cgtctgcggg tacagtaatt aaggtggatg
    25261 tcgcgttatg gagaggattg ttgttactct cggggaacgt agttacccaa ttaccatcgc
    25321 atctggtttg tttaatgaac cagcttcatt cttaccgctg aaatcgggcg agcaggtcat
    25381 gttggtcacc aacgaaaccc tggctcctct gtatctcgat aaggtccgcg gcgtacttga
    25441 acaggcgggt gttaatgtcg atagcgttat cctccctgac ggcgagcagt ataaaagcct
    25501 ggctgtactc gataccgtct ttacggcgtt gttacaaaag ccgcatggtc gcgatactac
    25561 gctggtggcg cttggcggcg gcgtagtggg cgatctgacc ggcttcgcgg cggcgagtta
    25621 tcagcgcggt gttcgtttca ttcaagtccc gacgacgtta ctgtcgcagg tcgattcctc
    25681 cgttggcggc aaaactgcgg tcaaccatcc cctcggtaaa aacatgattg gcgcgttcta
    25741 ccagcctgct tcagtggtgg tggatctcga ctgtctgaaa acgcttcccc cgcgtgagtt
    25801 agcgtcgggg ctggcagaag tcatcaaata cggcattatt cttgacggtg cgttttttaa
    25861 ctggctggaa gagaatctgg atgcgttgtt gcgtctggac ggtccggcaa tggcgtactg
    25921 tattcgccgt tgttgtgaac tgaaggcaga agttgtcgcc gccgacgagc gcgaaaccgg
    25981 gttacgtgct ttactgaatc tgggacacac ctttggtcat gccattgaag ctgaaatggg
    26041 gtatggcaat tggttacatg gtgaagcggt cgctgcgggt atggtgatgg cggcgcggac
    26101 gtcggaacgt ctcgggcagt ttagttctgc cgaaacgcag cgtattataa ccctgctcaa
    26161 gcgggctggg ttaccggtca atgggccgcg cgaaatgtcc gcgcaggcgt atttaccgca
    26221 tatgctgcgt gacaagaaag tccttgcggg agagatacgc ttaattcttc cgttggcaat
    26281 tggtaagagt gaagttcgca gcggcgtttc gcacgagctt gttcttaacg ccattgccga
    26341 ttgtcaatca gcgtaacaac aagaaaggtc aggccgctta tcaagcggtc tattagcttc
    26401 aggttaattg caacgtggta agcattaacc ttttagtggg gtgttaaatg gatgaattca
    26461 aaccagaaga cgagctgaaa cccgatccca gcgatcgtcg tactggtcgt tctcgtcaat
    26521 cttctgaacg ttctgagcgt actgaacgtg gcgaaccgca gatcaatttt gatgatattg
    26581 aacttgatga cactgacgat cgccgtccga ctcgtgcgca aaaagagcgc aatgaggaac
    26641 cggaaatcga agaagaaatt gacgaatccg aagatgaaac cgtggatgaa gagcgcgtag
    26701 agcgtcgtcc gcgtaagcgc aaaaaagcag ccagtaaacc cgcttctcgt cagtatatga
    26761 tgatgggcgt cggcattctg gttctactgc tgttgatcat cggtatcggt tctgcgctaa
    26821 aagccccctc gacctcttcc agcgatcaaa ccgcgtctgg cgagaagagt attgatcttg
    26881 caggcaatgc gaccgatcag gcgaatgggg tgcagccagc gccgggaacc acgtctgcgg
    26941 aaaatactca gcaggatgtt tctctgccac cgatctcttc tacgccgact caagggcaaa
    27001 ccccggcggc aacggatggt caacaacgtg ttgaagtgca gggtgacctg aacaatgcgc
    27061 tgacccagcc acaaaatcag caacagttga acaatgtggc ggtcaattcc acattgccga
    27121 ccgaacccgc gactgtcgcg cctgttcgca atggcaatgc atcgcgtgac acggcgaaaa
    27181 cgcaaaccgc tgaacgtccg tccactacgc gcccagctcg tcagcaggcg gtgattgaac
    27241 cgaaaaaacc gcaagcaacc gtgaaaacgg agccgaagcc ggtagcacag acgccgaagc
    27301 gtactgaacc agctgcccct gtggcgagca cgaaggcacc gactgcgact tctacgccag
    27361 caccaaaaga gacggcgact acggctccag tacagacggc atccccggcg caaaccacgg
    27421 caacaccagc cgctggaggg aagaccgcag gtaatgttgg ttcgttgaaa tcggcaccgt
    27481 ccagccatta cactctgcag ctgagcagtt cctctaacta cgacaacctg aacggttggg
    27541 cgaagaaaga gaatctgaaa aactacgttg tctatgaaac ggcgcgtaat ggtcagccgt
    27601 ggtatgtcct ggtttctggc gtgtacgctt cgaaagaaga ggcgaaaaaa gcggtatcta
    27661 cattgccagc agatgttcag gccaaaaacc cgtgggcgaa accgctgcgt caggtacagg
    27721 ccgatctgaa gtaatcaagg ttatctcccg caatggttta tcgttgcggg agctgcctga
    27781 agcgctggat gctgtcggag ctttctccac agccggagaa ggtgtaatta gttagtcagc
    27841 atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct gcttgatgat
    27901 attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg
    27961 gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag cgacctgatc
    28021 agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc acgcgagctg
    28081 tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa
    28141 agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta cggttacaac
    28201 ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgtttggccg ctacaaaaaa
    28261 acctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc
    28321 tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt cgtctattgc
    28381 gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca cacaaacagt
    28441 tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt tgagcgccat
    28501 attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta tcagcgcgca
    28561 aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac acgtaaaaag
    28621 gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa aaaataattc
    28681 tcaaggagaa gcggatgaaa cagtatttga ttgccccctc aattctgtcg gctgattttg
    28741 cccgcctggg tgaagatacc gcaaaagccc tggcagctgg cgctgatgtc gtgcattttg
    28801 acgtcatgga taaccactat gttcccaatc tgacgattgg gccaatggtg ctgaaatcct
    28861 tgcgtaacta tggcattacc gcccctatcg acgtacatct gatggtgaaa cccgtcgatc
    28921 gcattgtgcc tgatttcgct gccgctggtg ccagcatcat tacctttcat ccagaagcct
    28981 ccgagcatgt tgaccgcacg ctgcaactga ttaaagaaaa tggctgtaaa gcgggtctgg
    29041 tatttaaccc ggcgacacct ctgagctatc tggattacgt gatggataag ctggatgtga
    29101 tcctgctgat gtccgtcaat cctggtttcg gcggtcagtc tttcattcct caaacactgg
    29161 ataaactgcg cgaagtacgt cgccgtatcg acgagtctgg ctttgacatt cgtctggaag
    29221 tggacggtgg cgtgaaggtg aacaacattg gcgaaatcgc tgcggcgggc gcggatatgt
    29281 tcgtcgccgg ttcggcaatc ttcgaccagc cagactacaa aaaagtcatt gatgaaatgc
    29341 gcagtgaact ggcaaaggta agtcatgaat aagtttgaag atattcgcgg cgtcgctttt
    29401 gatcttgatg gtacgctggt cgacagtgct cccggtcttg ctgctgcggt agatatggcg
    29461 ctgtatgcgc tggagttgcc catcgcaggt gaagaacgcg ttattacctg gattggtaac
    29521 ggcgcagatg ttctgatgga gcgcgcattg acctgggcgc gtcaggaacg tgcgactcag
    29581 cgtaaaacaa tgggtaaacc gcccgttgat gacgacattc cggcagaaga acaggtacgt
    29641 attctgcgta aactgttcga tcgctactat ggcgaggttg ccgaagaggg gacgtttttg
    29701 ttcccgcacg ttgccgatac gttgggcgcg ttgcaggcta aaggcctgcc gctaggcctg
    29761 gtcaccaaca aaccgacgcc gttcgtcgca ccgctgctcg aagccttaga tattgccaaa
    29821 tactttagcg tggttatcgg cggcgatgat gtgcaaaaca aaaaaccgca tccggacccg
    29881 ctgttactgg tggctgagcg gatgggaatt gccccacaac agatgctgtt tgtcggcgac
    29941 tcacgcaatg atattcaggc ggcaaaagcg gcaggttgcc catcagttgg cttaacctac
    30001 ggatataact acggcgaggc tatcgatctc agccagcctg atgtaattta tcagtctata
    30061 aatgaccttc tgcccgcatt agggcttccg catagcgaaa atcaggaatc gaaaaatgac
    30121 taagcccatc gtttttagtg gcgcacagcc ctcaggtgaa ttgaccattg gtaactacat
    30181 gggtgcgctg cgtcagtggg taaacatgca ggatgactac cattgcattt actgtatcgt
    30241 tgaccaacac gcgatcaccg tgcgccagga tgcacagaag ctgcgtaaag cgacgctgga
    30301 tacgctggcc ttgtatctgg cttgtggtat cgatcctgag aaaagcacca tttttgttca
    30361 gtcccacgta ccagaacatg cgcagttagg ctgggcactg aactgttata cctacttcgg
    30421 cgaactgagt cgcatgacgc agtttaaaga taaatctgcg cgttatgccg agaacatcaa
    30481 cgctggtctg tttgactatc cggtgctgat ggctgcggac atcctgctgt atcaaactaa
    30541 tctggtaccg gtgggtgaag accagaaaca gcacctggaa ctgagtcgcg atatcgccca
    30601 gcgtttcaac gcgctgtatg gcgagatctt taaggtgccg gagccgttta ttccgaaatc
    30661 tggcgcgcgc gtaatgtcgc tgctggagcc gaccaagaag atgtccaagt ctgacgataa
    30721 ccgcaataac gttatcggcc tgctggaaga tccgaaatcg gtagtgaaga aaatcaaacg
    30781 cgcggtcact gactccgacg agccgccggt agttcgctac gatgtgcaga acaaagcggg
    30841 cgtttccaac ctgctggata tcctttctgc ggtaacgggc cagagcatcc cagaactgga
    30901 aaaacagttc gaaggcaaga tgtatggtca tctgaaaggt gaagtggctg atgccgtttc
    30961 cggtatgctg actgaattgc aggaacgcta tcaccgtttc cgcaacgatg aagccttcct
    31021 gcaacaggtg atgaaagacg gcgcagaaaa agccagtgta cacgcttccc gtacgctgaa
    31081 agcggtgtac gaagcgattg gttttgtggc gaagccgtaa tttaacatta atgccaaaaa
    31141 ccggggaaac ccggtttttt tatcctcatt tgcaataatc cgaaaaaatg tgaagcgcct
    31201 cgccgtttcc acatctcgtc gttgccactt atcttcattt taatgaagat ataaatattc
    31261 attttattga aaatatatta tgcgtcgaac gtttatcaaa aaagaaggcg tcgtcatcac
    31321 gacgctggcc cgttatttgt tgggtgaaaa atgcggtaat cgactgaaaa ccatagatga
    31381 gctggcaact gaatgccgtt catcggttgg cctgacgcag gccgcgttga aaacgttgga
    31441 atcaagcgga gcgatacgga ttgaacgccg tgggcgcaat ggcagttatc tggtcgagat
    31501 ggataacaaa gcattgctga gtcatgtgga tatcaacaac gtggtatgtg cgatgccttt
    31561 gccctatacc cgcttgtacg aaggtttagc gagcgggctg aaagctcagt ttgatggcat
    31621 tcctttttac tatgcgcata tgcgcggcgc ggatattcgc gtggagtgtt tacttaacgg
    31681 cgtgtatgac atggcggtgg tttcccgact ggcggcggaa agttatctca cgcaaaaagg
    31741 gttatgcctc gcgctggagt tggggccgca cacctacgtt ggcgagcacc agttgatttg
    31801 ccgtaaaggc gagtccgcaa acgtgaagcg cgtggggctg gataaccgtt cggcggatca
    31861 gaaaatcatg accgatgtct tttttggcga tagtgatgtg gaacgtgtcg atctctctta
    31921 tcacgagagt ttacaacgca ttgttaaagg cgatgtcgat gcggttatct ggaacgtggt
    31981 ggcggaaaac gaactgacca tgctgggatt agaggcgacg ccgctcacag acgatccgcg
    32041 atttttacag gccaccgaag ccgtggtcct gacgcgagtc gatgattacc caatgcaaca
    32101 actgctgcgt gccgttgtag ataaacacgc cctgctcgcc catcaacaac gggtagtgag
    32161 tggggaacag gaaccgagtt attaaacgaa aggcatctga tatggaaacc agactcaacc
    32221 tgctttgcga ggcaggcgtt attgataagg acatctgcaa aggcatgatg caggtcgtca
    32281 acgtactgga aacagagtgc catctgccgg tgcgcagtga gcaaggaacg atggcgatga
    32341 cacatatggc gagtgcactg atgcgcagtc gccgtggtga agaaatagag ccgctggata
    32401 acgaattgct ggcagagctg gcgcaatcca gccactggca agccgttgtg caattgcatc
    32461 aggtgttgtt gaaggaattc gcactggaag ttaacccgtg tgaagaaggc tatttgctgg
    32521 ccaaccttta tggattatgg atggctgcta acgaagaggt ttgaagctcc cgacaaatcc
    32581 acgtcgcaca acctaaatat tcaaaaaaat gaatatttaa ttcaaggaac agaaacgagg
    32641 caacaagatg tttgtagaag cattgaaacg ccagaacccg gcgctgattt ccgccgcact
    32701 aagtttgtgg cagcagggca agatcgcccc ggacagctgg gtgatcgacg tggatcaggt
    32761 actggaaaac ggtaagcgac tgattgagac ggcgcggctt tacggcattg aactgtatct
    32821 gatgaccaag cagtttggcc gtaacccatg gctggcggaa aaattgctgg cattaggtta
    32881 cagcggcatt gtggcggtgg attacaaaga ggcgcgagtc atgcgccgcg ctggtttgtc
    32941 ggtggcacat caggggcatc tggtacaaat cccttgtcat caggttgctg acgccgttga
    33001 acagggcacc gacgtcatca ccgtgtttac tctcgacaaa gcgcgggaag tttctgcggc
    33061 agcggtgaag gccggacgtg tgcagtttgt gctgctcaaa gtttatagcg acgatgattt
    33121 tctttatccg gggcaggaga gcggttttgt acagcattcg ctgcacgagg tggttgctga
    33181 aattaagaaa ctgccggggc tgcatttagc cggacttacc catttccctt gcctgctttg
    33241 ggatgaggct gccggaaaag ttttgccgac accgaatctt cacacgctga tacaggcacg
    33301 ggatcaactg gcgaaatctg gtattgcact tgagcaactg aacgcgcctt cagcgaccag
    33361 ctgcacttcg ctgccattac tggcgtaata cggtgtgact catgccgaac ccggtcatgc
    33421 gctgacgggc actattccgg caaaccagca gggcgatcag tctgaacgta tcgcgatgct
    33481 ctggttgagt gaaatctccc atcatttccg tggcgacagc tactgctacg gcggcggtta
    33541 ctaccgtcgt ggtcatgcgc aacatgcgtt ggtgtttacg ccagaaaatc aaaagattac
    33601 tgaaaccaat ctcaaaactg tggatgacag cagtatcgac tacaccctgt cgctggcagg
    33661 cgagtttccg gtaagcagtg cagtggtgct ctgtttccgc acgcagattt ttgtcacccg
    33721 tagcgatgtg gtgctggtgt ccggtattca ccgtggcgaa ccgaaaatcg ttggtcgtta
    33781 tgacagtctt ggaaactctc tgggggcgta atggcgcgat ttgtggtgtt agtgattgat
    33841 agctttggcg taggggcaat gaaagatgtc acgctggtgc gtccgcaaga tgcgggagcg
    33901 aatacatgtg gtcacatcct gagccagttg ccgcatttgc agctaccaac gctggagaag
    33961 ctggggctaa tcaacgcatt gggttatgcg ccaggcgata tgcagccgtc agattccgca
    34021 acctggggcg tggcagagct gcaacatgaa ggtggcgata cctttatggg gcatcaggaa
    34081 attttaggca cgcgcccgtt accgccgctg cggatgcctt ttcgcgatgt gattgaccgt
    34141 gttgagcagg cattagtatc cgccggttgg caggtggagc gccgtggcga tgaactgtaa
    34201 tttctgtggg tcaatcaggc ggttgcgatt ggcgataatc tcgaggcgga tttaggccaa
    34261 gtctataaca ttaccgccaa tctctctgtg atctcttttg acgacgcaat caaaattggt
    34321 cgtatcgtgc gtgagcaggt acaggtcggt cgggtcatta catttggtgg cctgttaacc
    34381 gacagtcaac gcattctcga tgccgcagaa agcaaagaag ggcgctttat tggtatcaat
    34441 gcgccgcgtt ctggcgctta tgacaacggc ttccaggtcg tgcatatggg ctacggtgtc
    34501 gataaaaaag tgcaggtgcc gcaaaaactg tatgaggcgg gcgtgccaac cgtgctggtg
    34561 ggtaaggtgg cagatatcgt cagcaatcct tatggcgtga gctggcaaaa cctggtggat
    34621 agccagcgga ttatggatat caccctcgac gaatttaaca cccatccgac ggcgtttatt
    34681 tgcaccaata tccaggaaac cgacctcgct ggtcatgcag aagacgtcgc acgttatgcc
    34741 gaacgtttgc aggtcgttga ccgtaacctt gcccggcttg ttgatgcgat gcagccagat
    34801 gattgcctgg tcgtgatggc ggatcacggc aacgatccga ccattggtca cagccaccat
    34861 acccgcgaag tggtgccgct gctggtttat cagcaaggcc tggctgctac gcagctcggt
    34921 gtgcgcacca cgctttctga tgtgggagct accgtgtgtg aatttttccg tgcgccaccg
    34981 ccacaaaatg gtcgctcttt tctttcctcc ctccggtttg caggagacac cctatgagtt
    35041 ttgatccgac gggttacacc ctggcccatg agcatctgca tattgatctc tccggcttta
    35101 aaaacaacgt ggactgccgc cttgatcagt atgcgttcat ttgccaggag atgaacgacc
    35161 tgatgacccg gggtgtacgt aatgtgattg agatgaccaa ccgttacatg gggcgcaatg
    35221 cgcaatttat gctcggtgta atgcgcgaaa ccgggatcaa cgtggtggcc tgtactggtt
    35281 attaccagga tgcgtttttc ccggaacatg tggtgacccg cagcgtgcag gaactggcgc
    35341 aggagatggt cgatgaaatt gaacagggta tcgatggcac ggagctgaaa gccgggatca
    35401 tcgcggagat cggctccagc gaaggaaaga ttacgccgct ggaagagaag gtatttattg
    35461 ccgctgcgct ggcgcataac cagaccggac gcccgatctc cacgcatacg tcgttcagca
    35521 ccatggggct ggaacaactg gctctgttgc aagcccacgg agttgatctt tcgcgcgtca
    35581 ccgttggtca ctgcgatctg aaagacaacc tcgacaacat tttgaagatg atcgatctcg
    35641 gcgcgtacgt gcagttcgac accattggta agaacagtta ctacccggac gaaaagcgca
    35701 ttgcgatgct ccacgcgcta cgtgaccgtg ggttgctgaa ccgcgtcatg ctgtcgatgg
    35761 atattacgcg ccgctcccat ttaaaagcca atggtggtta tggctatgac tatttattga
    35821 ccacctttat tccgcaattg cgccagtcag gattcagtca ggccgatgtg gatgtgatgt
    35881 tacgtgaaac tccctctcaa tttttccaat aaggacagac tcatgaaaaa gattggcgtt
    35941 gcaggcttac agcgtgagca gattaaaaaa accattgaag cgacggctac tggcagtttt
    36001 gaagttttca ttcacaacga catggaagcg gcaatgaaag tgaaatccgg gcaactggat
    36061 tactacatcg gtgcgtgtaa taccggtgcg ggcgcggcat tgtcgattgc catcgcggtg
    36121 attggctata acaaaagttg caccattgcc aaaccaggca ttaaagcgaa agacgagcat
    36181 atcgccaaaa tgatcgctga aggaaaagtg gcgtttggcc tttccgttga gcacgtcgaa
    36241 cacgcgattc cgatgctgat taaccatctg aaataaaagg cacgactatg gatctgtata
    36301 ttcagattat cgtggtggcg tgcctgacgg gtatgacatc gcttctggcg catcgctcgg
    36361 cggctgtttt tcatgacggt atccgcccga tcctgccgca actgattgaa ggctatatga
    36421 accgtcgcga ggcggggagt atcgcttttg gtctgagcat tggttttgtg gcctcggtgg
    36481 ggatctcttt taccctgaaa accgggctgc tcaacgcatg gttactcttt cttcctaccg
    36541 atatcctcgg cgtactggcg ataaacagcc tgatggcgtt tggtcttggc gctatctggg
    36601 gcgtgttgat ccttacttgc ctgttgccag taaaccagct gctgaccgcg ctgccggtgg
    36661 atgtattagg tagcctcggg gaattaagct cgccggtggt ttctgctttt gcactgttcc
    36721 cgctggtggc gattttctac cagtttggct ggaagcaaag tctggtcgcc gccgtggttg
    36781 ttctgatgac ccgtgtggta gtcgtgcgct atttcccaca tcttaaccct gaatccatcg
    36841 aaatctttat tggcatggtg atgctgctgg ggatcgcgat aactcacgac ctgcgtcatc
    36901 gtgatgaaaa tgatattgat gccagcgggc tttcggtgtt tgaagaacgc acgtcgcgga
    36961 ttatcaaaaa cttaccctat atcgccatcg tgggagcatt gattgccgcc gttgccagca
    37021 tgaagatttt cgccggcagt gaagtgtcga tcttcactct ggagaaagcg tactccgcag
    37081 gcgtaacgcc ggaacaatcg caaacgctga tcaaccaagc ggcactggca gaatttatgc
    37141 gcgggctggg ttttgtaccg ttgattgcca ccaccgcctt agcaacgggt gtgtatgcag
    37201 ttgcgggctt tacctttgtt tatgcggtgg gctatctctc gccgaatccg atagttgcag
    37261 cggtattagg cgcagtggtt atttcggcgg aagtcttgct gcttcgttcg atcggcaaat
    37321 ggctgggacg ctacccgtcg gtgcgtaatg cgtcggataa catccgtaac gccatgaata
    37381 tgctgatgga agtggcgctg ctggtcggtt cgattttcgc agcaattaag atggcgggtt
    37441 ataccggatt ctctatcgcg gttgccattt acttcctcaa cgaatccctg ggccgtccgg
    37501 tacagaaaat ggcggcaccg gtcgtggcag taatgatcac cggtattctg ctgaatgttc
    37561 tttactggct tggcctgttc gttccggctt aaggaaggca cctatgaaga cgtttcctct
    37621 gcaaagtctg acgattattg aggcgcagca aaagcagttt gcgctggtgg atagcatttg
    37681 tcgccatttc cccggcagcg agtttcttac tggcggtgat ttaggcttaa cgccaggact
    37741 gaatcaaccg cgcgttaccc agcgtgtgga acaggtgctg gctgatgcat ttcacgcaca
    37801 ggctgcggcg ctggtgcagg gtgcgggtac tggcgcgatt cgtgcggcgt tggcggcttt
    37861 gctcaaaccg gggcagcgtc ttctggtaca tgatgcgcct gtttacccga cgacacaggt
    37921 cattattgag cagatggggc tgacgcttat tactgctgat ttcaatgacc tgtcggcact
    37981 gaagcaggtc gtcgacgagc aactaccgga tgcggcgctg gtgcagcata cgcgccagca
    38041 gccgcaggac ggctacattc tggcagatgt gctggcaaca ttgcgctcgg ctggcgttcc
    38101 tgcattaacc gatgacaact atgcggtgat gaaagtggcg cgaattggct gcgaatgcgg
    38161 ggcaaatgtc tcgacttttt cctgcttcaa gctgtttggg ccggagggtg ttggcgcggt
    38221 ggtaggcgat gccgatgtta ttagtcggat tcgctccacg ctctattccg gcggcagcca
    38281 gattcagggc gctcaggcgc tggaagtctt gtgtgggctg gtgtttgcgc cagtgatgca
    38341 cgcggtgcag gcgggggtat ctgaacggtt gctggctttg cttaacggtg gtgcggtgcc
    38401 ggaagtgaaa agcgcggtga ttgctaatgc gcagtcgaag gtgttgattg tcgagtttca
    38461 tcagccgatt gccgccagag tgctggaaga ggcgcaaaag cgcggtgcct tgccttaccc
    38521 ggtgggtgca gagtcgaaat atgaaatccc gccgctcttt tatcgccttt ccggaacgtt
    38581 tcgccaggcg aatccacaat cagaacattg tgcgattcgc attaacccga atcgcaacgg
    38641 tgaagagacg gtgctgcgga ttttgcgtga gagtattgcc agtatttaat gctggatagc
    38701 accgtcaagc taaattccgt actgaacggt cccctcgccc ctttggggag agggttaggg
    38761 tgaggggaac gtacgggatg taaaattcaa cgcgccagca tcgccggatg cgatgctggc
    38821 gcatcttatc cggcctacgg ttaatgtctt ttattatcaa tcgttaaggt gatgcgattg
    38881 gcgcggcaga agagttcgga gacgtgcacc ggtttgtcat tctgatcaaa ggcgattttg
    38941 ctgatgcgaa acaacggttc gcccagttcg catttcagcc attttgcctg cgggcgggtg
    39001 gcggcaaaga tatcgatggt ctttttgtcg ctgaccactc gcgtgtcaaa acgctcctga
    39061 aataactgat aggtggagct tccttcgacg taaatctcat caaagtcagg ataacgcgac
    39121 agcgggatcc atgaactatc aataaacagc ggctctttat cgagatacat cacccggcag
    39181 agatggaaca cttcgctgtt tccggggatg ttcagctttt cgcaaaacgg cgcggcgctg
    39241 acccgttcct gttcgatcac tttctctttc gtcgccttgc cttgtgagac gccaaaatcg
    39301 gtaaaaccac tgacagtaag cagggcgttt tcaactttct ggctttgtac aaaggtgcct
    39361 ttgccctgcc agcggatcag tacgccgtct gccactaagt cgctgatagc tttgcgaata
    39421 gtaatgcggc tgacgttata ttgcgtacaa agctcgtttt cggtagggat ctgttgcccg
    39481 gcctggtaaa ccccctgcgc gatatcatcc agcagccgct ggcggacggt agcgtagagg
    39541 agttgatgag agtagcggtc cgtagctgac ataatcgacc ctgaaataaa gaatgaatta
    39601 cacggattat agggtcgttt tccggcaagt aacagcacgt tggcgcggag atgctcacgc
    39661 caacgttata cctaccaggc accgtgatac tgaatggttt tcgccgcgca cgccgttccc
    39721 tgcgccatcg cctgcggtaa tgtcatcccc gcagaccagc cgcaaaggaa tccggcaatg
    39781 aacgaatctc cggcacccat ggtgtcgata accgtcaccg gttcaggagc ctgacgccag
    39841 aactgcgcgc catcccaggc aatgctgccg ttttcaccca gcgtgacaat cactgttcct
    39901 gcgccacggg caacaatcgc tttcatcttc agacgcagcg cttcgtcttc ttgcggtgcg
    39961 gaggcaaagg caaaatcgag atgcggcacc agtgtctgcc agagcgggct gtcccacttg
    40021 tcggagaagt cgaaagcggt aagtttgccc gcagcgtgca gctgtgggaa tgcgtcttcc
    40081 gcatgtcccc agattgccgc gtgcacaatg tcatactgcg ccagccaggc gtaatcctct
    40141 tcactcaggg caaagtcggc catcacgcct tcggtgtagt cgccaaaaac gcgatcattg
    40201 tcgtgcagtt ccacctgagt ttgtgcggta acgccgtgtt tcgtatggac atggctgata
    40261 tcgacgccca tgcgggcgag atcctgcttc agctttgtgc cgtagtcatc gtcacccacc
    40321 aaggtaatgc atcccggctg tatgccgtag cgagtgcagt acaccgccac attgaccgca
    40381 ttaccgccag aaaacgcttt attcagttgc gggtagatat cgacgcagtt atcgccgatt
    40441 gtcgccaggg ttttcatctc tcatcctccg gcagcagcgc gcgaaagcgt tccagcgcct
    40501 ggcgggcata gagtctgggc tcgttcatat acatcgtcac cagctccacc gtacagtaac
    40561 cctcatagcc ccgctcaata atatcgcgca tcagttcccg cagcggcatt ttgccttctc
    40621 caggaatgta atgcgtgtcg ctggccccgt cgctgtcgac aatatgcaga tgacgataat
    40681 ttatcgccca gtttgtcgaa ataactcatc accggttccg cctggacata cggcgcgcaa
    40741 atgtcgacca tgctgaacag gcgcggcgaa ggcaccagcg ccagcgcatg aagcacatca
    40801 ttagcattac acacgacgtt cgattcatac ggcgttaacg gttcgaggat tagatccatg
    40861 ccgatgtttt ccgcgtactc acacagttcg cttaggttct ctgccagccg tccccagata
    40921 acattaggtg gcgtgagata gcctgcgtgg gccgcggaaa tcagcgtata acccgcgttc
    40981 atctcttttg ccatatccat cgccagcttg atcatgtcga ggctttcgcg acgcatatgt
    41041 tcatcgccca gcatcatgtt atacggatag ccgttggttt ctggcgtata gccgataatc
    41101 ggcatctgat acgtctgcgc cagcgccttg atttgtttga tgccgcccgc ttttaagtcc
    41161 ggcgcgaacg cgtgcgggcg accgccccaa atttcgatgc cgtcgtaacc cagctcgctt
    41221 gcatcacgaa atgcgtgttc aataggcagc cgctggtggc cgcaggtaaa catacctgtt
    41281 ttcatcgggt aactccttgc gtaaatccgg cccgttgccg ggccgggaga gggattaata
    41341 ttccaccaga ccaccgtaat agcggcgttc atccgggttg tgatctttgt aaatagacag
    41401 gtagtagcag agccactcca ttggcacgaa catcaggaac ggtgccagcc acgggtgcag
    41461 cccttgcgaa atttcggcgt aatcgatgac gatcacgttg tcagtacgct gtttaacaaa
    41521 gttaatggcg cattcggtgg tgtggcgact ttcatcattg ccgagcagga acaggaacgg
    41581 aacgcccggt tcgacaatct ccagcgggcc atggcggaac tctccgctct caatcacgca
    41641 gccgtgcgtc caggtaaatt ccatcagcgt tacaatgcct tctttgtaac ccagcggacg
    41701 cagcggaccc gcagcaacgg tataaatcat cggccactgg ctggccagtt caccaagctg
    41761 gcggcctttt tcttcccagg tgcgtaccag atgaccgagc gcattcggca acggcttgag
    41821 atcgtttttg atcttgccga tttccgcgtt cggcgcgagg cgggtgatca tctccagcac
    41881 cacgctgtag cagagcagca ggtgaatttc ccagatacag tcggcctgat aatcaatgct
    41941 aaattccgcc gccgaggtaa tcgggctgtc cgcgcgtttg gtgaacgccg cagtgagtgc
    42001 gccgcaggcc cggcccagct ccagcgcttt gattacctct tccgttttac cgtagtcaga
    42061 aacgccaatc accgcgcaac gatcgtcgag gcgatacggg gtgttatcgc agaactccca
    42121 gccggaaatg gcgtagacct gaagatcgga aaagcgatcc gccagatgtt tcgccgtttg
    42181 cgccgcgttg agtggcgagc cgcaggcaac gaaataaata cgatcaatgt cgcgcttcac
    42241 catctcttcc acgatggcgt gcaccagcgg aacgtcatgg ctgagaactt tttccacttc
    42301 ctgaaccata ttttcggtga ccagaaagtc cacggtgctt ttatcaatat ccaacatttt
    42361 attttctcca gataacatta cgacaaagcg ttgagctggc ggctacgctt cgcccagaag
    42421 gcgtaagcag gcagaccagt agcaataacg atgacggcgc agataaggcc gggaatcggt
    42481 gcccagacaa aggttgaggc gaccagaatg aggcttgacg caatggcgag ggtggtcatc
    42541 agcccgaaag ccggagtacg ccacagcggt ttgtaatcgt cgcgtttacg acaccagatg
    42601 atggagccga aggtgagggt atttttgaaa cacatcacca gggtgaaata acccagcagg
    42661 ctggtgagat cggaaacgaa gatgaagaag atccccagcg ccccttgcag gatgatggag
    42721 acatccggcg tgttgtattt cggatgcaca tggccgaagc atttaaagaa caggttgtct
    42781 ttcgccatcg cgtattccag ccgcggctgg tacatcacgc agctggaaag cgaaccaaga
    42841 atgacgatca tcgccgtgat ggcaacaaag atcccagcgg tgctgccgag tgcggggatc
    42901 caggtcaggg cgtcggaaat cggcgtttca gaattggcga gtttgtcgaa gggcatcagg
    42961 ccggaaatca ccagcgccag cagggtgtag agcaccagaa ccagcagaca ggaaccaatc
    43021 agcgctcgtg gcatggtttt tccggggttt ttaatttcgc cggtcatata acagatagag
    43081 gccatgccgg tatacgacca actggtggca gagatccccg ccagcagcgc cataaagctg
    43141 cccgttgcgc caatcgcagt ggtggtaggg gcggcaaaat tctccgcttt aaaccagaag
    43201 atcccaaggc caatgacgat agtgaacggg ataattttgg cgatggtaat tagcgtctga
    43261 aacgctgcgc cgccttcaac ggagcgcagg tgtagcaaca taaaggcgat aattaatccg
    43321 gcggcgataa atttaccgag caacggatcg ataggcgtta aaaagccaag attgctgaca
    43381 atcgccagcg ccataatcga caatgacggc gcatcgttgg cccagaagct ggcccagccg
    43441 gagaggaaag ccagcggtcg gcttccggca tttttcagat aaacataatc tgcgccattt
    43501 tccggataag cggtggatag ttccgcatag ac
//
LOCUS       DAJVRF010000014        43477 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJVRF010000014 DAJVRF010000000
VERSION     DAJVRF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43477
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.014"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(1..77)
                     /locus_tag="OQN13_000729"
     tRNA            complement(1..77)
                     /locus_tag="OQN13_000729"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Arg,seq:acg)
     gene            complement(81..173)
                     /locus_tag="OQN13_000730"
     tRNA            complement(81..173)
                     /locus_tag="OQN13_000730"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(137..139),aa:Ser,seq:gct)
     gene            complement(489..674)
                     /gene="csrA"
                     /locus_tag="OQN13_000731"
     CDS             complement(489..674)
                     /gene="csrA"
                     /locus_tag="OQN13_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCS1614180.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(909..3539)
                     /gene="alaS"
                     /locus_tag="OQN13_000732"
     CDS             complement(909..3539)
                     /gene="alaS"
                     /locus_tag="OQN13_000732"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCS1614181.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3667..4167)
                     /gene="recX"
                     /locus_tag="OQN13_000733"
     CDS             complement(3667..4167)
                     /gene="recX"
                     /locus_tag="OQN13_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCS1614182.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WLNFAD"
     gene            complement(4395..5456)
                     /gene="recA"
                     /locus_tag="OQN13_000734"
     CDS             complement(4395..5456)
                     /gene="recA"
                     /locus_tag="OQN13_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCS1614183.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5536..6033)
                     /gene="pncC"
                     /locus_tag="OQN13_000735"
     CDS             complement(5536..6033)
                     /gene="pncC"
                     /locus_tag="OQN13_000735"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCS1614184.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6178..7263)
                     /gene="mltB"
                     /locus_tag="OQN13_000736"
     CDS             complement(6178..7263)
                     /gene="mltB"
                     /locus_tag="OQN13_000736"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCS1614185.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7519..8082
                     /gene="srlA"
                     /locus_tag="OQN13_000737"
                     /gene_synonym="gutA"
     CDS             7519..8082
                     /gene="srlA"
                     /locus_tag="OQN13_000737"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCS1614186.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8079..9038
                     /gene="srlE"
                     /locus_tag="OQN13_000738"
                     /gene_synonym="gutE"
     CDS             8079..9038
                     /gene="srlE"
                     /locus_tag="OQN13_000738"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCS1614187.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            9049..9420
                     /gene="srlB"
                     /locus_tag="OQN13_000739"
                     /gene_synonym="gutB"
     CDS             9049..9420
                     /gene="srlB"
                     /locus_tag="OQN13_000739"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCS1614188.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9424..10192
                     /gene="srlD"
                     /locus_tag="OQN13_000740"
                     /pseudo
     CDS             9424..10192
                     /gene="srlD"
                     /locus_tag="OQN13_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            10298..10657
                     /gene="gutM"
                     /locus_tag="OQN13_000741"
     CDS             10298..10657
                     /gene="gutM"
                     /locus_tag="OQN13_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCS1614189.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10724..11497
                     /gene="srlR"
                     /locus_tag="OQN13_000742"
                     /gene_synonym="gutR"
     CDS             10724..11497
                     /gene="srlR"
                     /locus_tag="OQN13_000742"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCS1614190.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11490..12455
                     /gene="gutQ"
                     /locus_tag="OQN13_000743"
     CDS             11490..12455
                     /gene="gutQ"
                     /locus_tag="OQN13_000743"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCS1614191.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12452..13966)
                     /gene="norR"
                     /locus_tag="OQN13_000744"
     CDS             complement(12452..13966)
                     /gene="norR"
                     /locus_tag="OQN13_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCS1614192.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            14153..15592
                     /gene="norV"
                     /locus_tag="OQN13_000745"
     CDS             14153..15592
                     /gene="norV"
                     /locus_tag="OQN13_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCS1614193.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15589..16722
                     /gene="norW"
                     /locus_tag="OQN13_000746"
     CDS             15589..16722
                     /gene="norW"
                     /locus_tag="OQN13_000746"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCS1614194.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(16850..19102)
                     /gene="hypF"
                     /locus_tag="OQN13_000747"
     CDS             complement(16850..19102)
                     /gene="hypF"
                     /locus_tag="OQN13_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCS1614195.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19255..19782)
                     /gene="hydN"
                     /locus_tag="OQN13_000748"
     CDS             complement(19255..19782)
                     /gene="hydN"
                     /locus_tag="OQN13_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCS1614196.1"
                     /translation="MNRFIIADASKCIGCCTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19930..20940)
                     /gene="ascG"
                     /locus_tag="OQN13_000749"
     CDS             complement(19930..20940)
                     /gene="ascG"
                     /locus_tag="OQN13_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCS1614197.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21200..22657
                     /gene="ascF"
                     /locus_tag="OQN13_000750"
     CDS             21200..22657
                     /gene="ascF"
                     /locus_tag="OQN13_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCS1614198.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22666..24090
                     /gene="ascB"
                     /locus_tag="OQN13_000751"
     CDS             22666..24090
                     /gene="ascB"
                     /locus_tag="OQN13_000751"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCS1614199.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24204..24623)
                     /locus_tag="OQN13_000752"
     CDS             complement(24204..24623)
                     /locus_tag="OQN13_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1614200.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(24620..24931)
                     /locus_tag="OQN13_000753"
     CDS             complement(24620..24931)
                     /locus_tag="OQN13_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HCS1614201.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25093..25866)
                     /locus_tag="OQN13_000754"
     CDS             complement(25093..25866)
                     /locus_tag="OQN13_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614202.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            complement(25891..26361)
                     /gene="hycI"
                     /locus_tag="OQN13_000755"
     CDS             complement(25891..26361)
                     /gene="hycI"
                     /locus_tag="OQN13_000755"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCS1614203.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(26354..26764)
                     /gene="hycH"
                     /locus_tag="OQN13_000756"
     CDS             complement(26354..26764)
                     /gene="hycH"
                     /locus_tag="OQN13_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCS1614204.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(26761..27528)
                     /gene="hycG"
                     /locus_tag="OQN13_000757"
     CDS             complement(26761..27528)
                     /gene="hycG"
                     /locus_tag="OQN13_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCS1614205.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(27528..28070)
                     /gene="hycF"
                     /locus_tag="OQN13_000758"
     CDS             complement(27528..28070)
                     /gene="hycF"
                     /locus_tag="OQN13_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCS1614206.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28080..29777)
                     /gene="hycE"
                     /locus_tag="OQN13_000759"
     CDS             complement(28080..29777)
                     /gene="hycE"
                     /locus_tag="OQN13_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCS1614207.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(29795..30718)
                     /gene="hycD"
                     /locus_tag="OQN13_000760"
     CDS             complement(29795..30718)
                     /gene="hycD"
                     /locus_tag="OQN13_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCS1614208.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            complement(30721..32547)
                     /gene="hycC"
                     /locus_tag="OQN13_000761"
     CDS             complement(30721..32547)
                     /gene="hycC"
                     /locus_tag="OQN13_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCS1614209.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCDYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(32544..33155)
                     /gene="hycB"
                     /locus_tag="OQN13_000762"
     CDS             complement(32544..33155)
                     /gene="hycB"
                     /locus_tag="OQN13_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCS1614210.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33280..33741)
                     /gene="hycA"
                     /locus_tag="OQN13_000763"
     CDS             complement(33280..33741)
                     /gene="hycA"
                     /locus_tag="OQN13_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCS1614211.1"
                     /translation="MTIWEISEKADYIAQRHCRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            33953..34303
                     /gene="hypA"
                     /locus_tag="OQN13_000764"
     CDS             33953..34303
                     /gene="hypA"
                     /locus_tag="OQN13_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS1614212.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            34307..35179
                     /gene="hypB"
                     /locus_tag="OQN13_000765"
     CDS             34307..35179
                     /gene="hypB"
                     /locus_tag="OQN13_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCS1614213.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            35170..35442
                     /gene="hypC"
                     /locus_tag="OQN13_000766"
     CDS             35170..35442
                     /gene="hypC"
                     /locus_tag="OQN13_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCS1614214.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            35442..36563
                     /gene="hypD"
                     /locus_tag="OQN13_000767"
     CDS             35442..36563
                     /gene="hypD"
                     /locus_tag="OQN13_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCS1614215.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            36560..37570
                     /gene="hypE"
                     /locus_tag="OQN13_000768"
     CDS             36560..37570
                     /gene="hypE"
                     /locus_tag="OQN13_000768"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCS1614216.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            37644..39721
                     /gene="flhA"
                     /locus_tag="OQN13_000769"
                     /pseudo
     CDS             37644..39721
                     /gene="flhA"
                     /locus_tag="OQN13_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(39758..40111)
                     /locus_tag="OQN13_000770"
     CDS             complement(39758..40111)
                     /locus_tag="OQN13_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCS1614217.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(40188..40322)
                     /locus_tag="OQN13_000771"
     CDS             complement(40188..40322)
                     /locus_tag="OQN13_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614218.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            40398..42959
                     /gene="mutS"
                     /locus_tag="OQN13_000772"
     CDS             40398..42959
                     /gene="mutS"
                     /locus_tag="OQN13_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCS1614219.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            43065..43477
                     /locus_tag="OQN13_000773"
                     /pseudo
     CDS             43065..43477
                     /locus_tag="OQN13_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
BASE COUNT        10292 a        11935 c        11328 g         9922 t
ORIGIN      
        1 tggtgcatcc gggaggattc gaacctccga ccgctcggtt cgtagccgag tactctatcc
       61 agctgagcta cggatgcaaa tggcggtgag gcggggattc gaaccccgga tgcagctttt
      121 gaccgcatac tcccttagca ggggagcgcc ttcagcctct cggccacctc accacacgcc
      181 tcttacgagt gcttcgaaga acttgtttat cgctcatcgt cgctgcgtgg cgcacatatt
      241 actttctgag acttataagt caaacaattt ttcccacact tttatcgttt gcacacttca
      301 cgctcaatta gtctgtaaaa acgtcaaaaa gagtgtttta tctacagaaa aacggaggtc
      361 tggcagatag cagtaatgca aaaaaatgga gacttaagtt gaatgaacgg gagtaaagcg
      421 aaaagactat agagtgaagg agaaattttg agggtgcgtc tcaccgataa agatgagacg
      481 cggaaagatt agtaactgga ctgctgggat ttttcagcct ggatacgctg gtagatctct
      541 tcacggtgaa cagaaacttc cttcggggca tttacgccaa tacgtacctg gttgcccttt
      601 acccctaaaa ctgtcacggt gacctcatcc ccaatcatga gggtctcacc aactcgacga
      661 gtcagaatca gcattctttg ctccttgaaa gattaaaaga gtcgggtctc tctgtatccc
      721 ggcattatcc atcatataac gccaaaaagt aagcaatgac aaacacatta catgtaagca
      781 gtcatggcat tacattctgt taaacctaag tttagccgat atacacaact tcaacctgac
      841 tttatcgttg tcgatagcgt taatgcgaat gccgtgaagc gagtccacgg cattgcctga
      901 cgcttatatt attgcaattt cgcgctgacc cagcctttca cactggctaa cgctgcaggt
      961 aaggccgcag catccgtacc accggcttgc gccatgtcag gacgtccacc acccttgccg
     1021 cccacctgct gagcgaccat accaatcagt tcccctgctt tcacacgatc tgtgacgtcc
     1081 ttagatacgc ctgcaatcag agaaacctta ccttcggcta ccgttgccag cacgataatt
     1141 gtcgacccca gctgattttt taaatcgtca accatggtac gcaacatttt cggctcaaca
     1201 ccgctaagct cgctaaccaa cagcttaaca ccattaacat caattgcctt actggaaaga
     1261 tttgcgctct cctgtgcggc agcttgttct ttaagctgtt gtagttcttt ttctaactga
     1321 cgcgtacgtt ccagtactga gcgcacttta tccgccagat tattgctatc gcctttcagc
     1381 agatgcgcga cttcgcttaa gcgatcgctg tctgcatgaa cggtggtgat agcgccttct
     1441 ccggttaccg cttcgatacg acgaacgcct gcagcagtac ccgattcaga gatgatgcgg
     1501 aacagaccaa tatcaccagt gcggctggcg tgagtaccgc cacacagctc ggtggagaaa
     1561 tcgcccatgc tcagcacgcg tacacgctca tcatacttct cgccgaacag cgccatcgca
     1621 cctttcgctt tcgccgcttc gagatccatg atgttggttt cgatcggcaa attgcgacga
     1681 atctgtgcgt tcaccaggtc ttcgaccgca cgaatctctt ccggtttcat cgcttcgttg
     1741 tgtgagaagt cgaagcgcag caccttgtcg ttaaccagtg aacctttctg cgatacatga
     1801 gtccccagaa cctggcgcag cgcagcgtgc atcaggtgcg ttgcggagtg attcagacga
     1861 atacgggcgc gacgagcctc atcaacatca gcctgcacag cgtcgcccac tttcagagaa
     1921 cccgcagcaa gtttaccgat gtgaccaatc gcctggccgt atttctgcgt atcttccacc
     1981 gcgaaggaga agttagcgcc tttcagttcg cctttatcac caacctgacc gccggattcc
     2041 gcatagaatg gcgtttgatc cagcacgacc acagcttcct ggcctgcatt gatggcatca
     2101 accgctttac catcaacaaa cagcgcggtc actttgccgt tcagttccag atggtcatag
     2161 cctttaaatt cagatgcact atcaacacgg atcattgcgt tgtaatcggc accaaagccg
     2221 ctggcttcgc gcgcgcgacg acgctgctct tccattgctg cttcaaaacc agcttcgtca
     2281 actttgatgt tgcgctcacg acaaacatca gccgtcaggt caaccgggaa gccataggtg
     2341 tcgtacagac ggaaagccgt ttcaccatcc agcgtatcac cagaaagttt tgccagctct
     2401 tcatccagca acgccagacc gcgctccaga gtacgggcaa actgctcttc ttcagtcttc
     2461 agcacctgct caacctgcgc ctgctggcgt ttcaggtctt cacccgcaga gcccataacg
     2521 tcgatcagcg gaccaaccag tttgtagaag aaggtttctt tcgcgccgag catattgccg
     2581 tgacgcactg cgcgacgaat gatacgacgc agtacataac cacggttttc attggacggc
     2641 attacgccat ccgcgatcag gaacgcacaa gaacgaatgt ggtcagcgat tacgcgcagc
     2701 gatttattgc tcagatcggt cgcgccagtg actttcgcta ccgcctggat caacgtgcgg
     2761 aacaggtcga tgtcatagtt agagttaacg tgttgcagca ccgcagcaat acgctccaga
     2821 cccataccgg tatctacaga cggcttcggc agcggttcca tcgtgccatc tgcctggcgg
     2881 ttgaactgca tgaagacgat gttccagatc tcaatgtagc ggtcgccgtc ttcttccgga
     2941 cttcccggag ggccacccca aatgtggtcg ccgtgatcgt agaagatttc ggtgcacggg
     3001 ccgcacggac cagtgtcacc catctgccag aagttgtcag atgcgtatgg cgcacctttg
     3061 ttatcgccga tgcgaataat acgttcgcgc gggatcccta cttctttttc ccagatttcg
     3121 taggcttcgt cgtcgctttc atagacggta acccacagac gctctttcgg cagggcaaac
     3181 catttttcgc tggtcagcag ttcccatgca aactgaatgg catcgtgttt gaaatagtcg
     3241 ccgaagctga agttgcccag catttcgaag aaggtatggt gacgcgcggt gtaaccgaca
     3301 ttttccaggt cgttgtgttt accacccgca cgcacgcagc gttgggaagt ggtagcgcgg
     3361 gaataattac gcttgtcgag tccaaggaac acatccttga actggttcat cccggcgttg
     3421 gtaaacaaca aagttgggtc gttatggggt accagggagc tgctggcaac tacctgatgt
     3481 cccttactat ggaaaaagtc gagaaacgcc tgacggatct cagcggtgct cttgctcata
     3541 attatcctga aatcaagcta acgaaatatc gccaccagct ccagcgtgtc ttaaccgccg
     3601 ggctggtaac tgaaaagtgg gaataagata agttttcttg agtgggaagt aaaataccgt
     3661 atgcgttcag tcggcaaaat ttagccaaat atcctggata tcttccatca gatagccacg
     3721 atagagcaga aaacgctgga tcttaacttt ttctgaaaag acagttggca aaggttcacc
     3781 atattttcgc gtcgcctgat cgcgcaccag tgcgcaccag tcgatgtcac attcacgcat
     3841 cgctttttct gtcgcttcgc gggaaatacc tttctgattc agttcctgac gaatacgtgc
     3901 aggtccataa cctttgcggc tacggctggc gataaagcgc gcaacaaatc ggctgtcatc
     3961 gagatagcca tgttcatggc accaggcaat aacgcgctcg taatcttctg ccgtagcatc
     4021 aatctcttct gggccatttt tgcccataat cggtgccgcg agtttacgtc gcagttcttg
     4081 ctcactgtga tcgcgcaccg ccagaatgcg taccgcacga tccaacaggc gagcatatgc
     4141 cgggcgacgg gatgttgatt ctgtcatggc atatccttac aacttaaaaa agcaaaaggg
     4201 ccgcagatgc gacccaggtt ttgctgataa acagatttaa tagtcaatga gtgtgcagcc
     4261 aattcgttag aggatcgagg tgagagtaat ttttcacgcc agtgttggcc tgttcccctc
     4321 accctaaccc tctccccaaa ggggcgaggg gaccgtccgt gcacacatta tctattatca
     4381 tcaataacga acgattaaaa atcttcgtta gtttctgcta cgccttcgct gtcatctaca
     4441 gagaaatccg gcgttgagtt cgggttgctc agcagcaact cacgtacttt cttctcgatc
     4501 tctttcgcgg tttccgggtt atctttcagc caggcagttg cattcgcttt accctgaccg
     4561 atcttctcac ctttgtagct gtaccacgcg cctgctttct cgatcagctt ctcttttacg
     4621 cccaggtcaa ccagttcgcc gtagaagttg ataccttcgc cgtagaggat ctgaaattca
     4681 gcctgtttaa acggtgcagc gattttgttc ttcaccactt tcacgcgggt ttcgctaccc
     4741 accacgtttt cgccctcttt caccgcgccg atacgacgga tgtcgagacg aacagaggcg
     4801 tagaatttca gcgcgttacc accggtagtg gtttccgggt taccgaacat cacaccaatt
     4861 ttcatacgga tctggttgat gaagatcagc agcgtgttgg actgcttcag gttacccgcc
     4921 agcttacgca tcgcctggct catcatacgt gccgcaaggc ccatgtgaga gtcgccgatt
     4981 tcgccttcga tttccgcttt cggcgtcagt gccgccacgg agtcaacgac gataacgtct
     5041 actgcgccag aacgcgccag ggcgtcacag atttccagtg cctgctcgcc ggtgtccggc
     5101 tgggagcaca gcaggttatc gatatcgacg cccagtttac gtgcgtagat tgggtccagc
     5161 gcgtgttcag catcgataaa cgcacaggtt ttaccttcac gctgcgctgc ggcgatcacc
     5221 tgcagcgtca gcgtggtttt accggaagat tccggtccgt agatttcgac gatacggccc
     5281 atcggcagac cacctgcccc aagcgcgata tccagtgaaa gcgaaccggt agagatggtt
     5341 tccacatcca tggaacggtc ttcacccagg cgcatgatgg agcctttacc aaattgtttc
     5401 tcaatctggc ccagtgctgc cgccaacgct ttctgtttgt tttcgtcgat agccattttt
     5461 actcctgtca tgccgggtaa taccggatag tcaatatgtt ctgttgaagc aattatactg
     5521 tatactcata cagtatcaag tgttttgtag aaattgttgc cacaaggtct gcaatgcata
     5581 cgcagtagcc tgacgacgca ccgcatcacg gtcgccgctg aagcattccc gccgggtaat
     5641 gccttcaccg cgggcagtgg caaaagcaaa ccagacggtg ccgacaggct tctcttcact
     5701 gccgccatcc ggcccggcga taccactaat agacacggca taatcagcac gagccgcttt
     5761 cagtgcacct atcgccattt ccaccacgac gggttcactc accgcgccat gctgcgccag
     5821 cgtctcttcg cgtacgccga tcatctgcgc tttggcttcg ttactgtagg tgacaaatcc
     5881 gcgttcaaac caggcggagc taccggcaat atcggtaatc actttcgcta cccaaccacc
     5941 ggtacaagac tcggcagttg ttacggttgc gccacgggct ttcagcgcct gcccaacctg
     6001 ttcgcttaac tgcatcagtt cactgtcagt catcacaacc tcagtcagtc aaaaatttat
     6061 gccggacaag atagcacttt cgtcaggaag atggtgttgg cgttgcaatt ttacgaggag
     6121 gattcagaaa aaagctgatt agccagaggg aagctcacgc ccccctcttg caaatagtta
     6181 ctgcacgcgc gccagcgcca cggcttgtcc taactgccag accgccattg cgtaatgggt
     6241 gctgtggtta taacgggtaa tggtgtagaa gttcggcaga ccgtaccagt actggtaacc
     6301 ggtaccgaca tccagacgca gcaggctggc ttgttgatgg ttgcccagcg gctgctgtgg
     6361 cgttaaacct gccgtggcaa gctgcgaaat gctgtacctg gttttgaagc catttggcaa
     6421 gcctggagcc tgaccgtttg ccattaccgc gacctgatcg cctttcaccc agccgtgcgc
     6481 tttgaaatag ttcgccacac taccgatcgc atcaaccgga tcccacaggt tgatatgccc
     6541 gtcgccgctg aaatctaccg catattgttt gtaagacgac ggcataaact gtccgtagcc
     6601 catcgccccg gcaaaggaac ctttcagatt gagcggatcg tcctgctcgt cgcgcgccat
     6661 caacaggaag gtttccagct cgccagagaa atactccgcg cggcgtgggt agttaaatga
     6721 cagcgttgcc agcgcatcga ggatgcgagt tttccccatc acgcgccccc agcgggtttc
     6781 aacgccgata atcccgacga taatttccgg cggtacgcca tacacctgcc acgcgcgatt
     6841 caacgcatct tcatactgat tccagaaaac cacaccgttc tgcacgttgt ccggcgtaat
     6901 aaattttttg cgataacgca gccatgcgcc gttcggacct gatggcggtt tcaccgatgt
     6961 ggttggtgcc tggttatcca tcagccgcag taccgaatcc agacgcttcg cctgggagag
     7021 aatttcctgc aactgctgac gatcgaaacc gtgtttgttc accattctgt cgatgaactg
     7081 ctgggcattc gggttattag cgaaatcgcc gcccatctgc atcacattgt gctgaggctc
     7141 aagcaggaag ccgccagacg gcgttccggt ggtcgtttca gtctcagtag gttttggctt
     7201 gctgctacag gcggcaagca acacaaaaag gggaagcaat gttacataac gacgcttgaa
     7261 catgaggggt ccatttaaca gattcaacca ggggcaagta tggtaaagca tcacgccccg
     7321 cacaaggaag cggtagtcac tgcccgatac ggactttaca taactcaact cattcccctc
     7381 gctatccttt tattcaaact ttcaaattaa aatatttatc tttcattttg cgatcaaaat
     7441 aacactttca aatctttcaa tctgattaga ttaggttgcc gtttggtaat aaaacaataa
     7501 atcctgaagg agagaacaat gatagaaacc attactcatg gtgcagagtg gtttatcggg
     7561 ctgttccaaa agggcggaga ggtgtttacc gggatggtga ccggcattct tccgctgttg
     7621 attagcctgc tggttatcat gaacgcactg attaatttta tcggtcagca tcgtattgaa
     7681 cgttttgctc aacgttgcgc cggtaaccct gtttcccgtt acctgctgct accgtgcatt
     7741 ggcacgtttg tcttttgcaa tccgatgacc ttaagcctgg gtcgctttat gccggaaaag
     7801 tacaaaccca gctactacgc ggcagcctct tatagctgcc actcaatgaa tggcctcttt
     7861 ccccatatca accctggcga actgtttgtt tatcttggca ttgccagcgg tctgacaacg
     7921 ctgaacctgc cacttggccc actggcggtg agctatctgc tggttggtct ggtcaccaac
     7981 ttctttcgcg gctgggtgac cgatctgacc accgccattt ttgagaaaaa gatgggcatt
     8041 caacttgaac aaaaagttca cctggcagga gcaacatcat gacgcgtatt cggatcgaaa
     8101 aaggaacggg tggctggggc ggcccgcttg agctggaagc cacgccgggc aaaaaaatcg
     8161 tctatatcac cgccggtacc cggcctgcga ttgttgacaa actggcacaa cttactggct
     8221 ggcaggctat tgacggattt aaagaaggtg aacccgcgga ggcggaaatt ggtgtcgcgg
     8281 taatcgactg tggcggcaca ttacgctgcg gcatctatcc gaaacgacgt attcccacca
     8341 ttaatatcca ctcgacgggc aagtccagtc cgctggcgca gtacattgtg gaagatattt
     8401 atgtctctgg cgtaaaagaa gaaaacatca ctgtagtggg tgatgcgaca ccacaaccct
     8461 cttccgtggg ccgtgactat gacaccagca agaaaatcac cgaacaaagc gatggtttac
     8521 tggcgaaggt gggaatgggt atgggttctg ccgttgccgt gttgtttcaa tctggtcgtg
     8581 acaccatcga cactgtatta aaaaccattc tgccgtttat ggcgttcgtc tcggcgctca
     8641 ttggcatcat tatggcttct ggccttggtg actggattgc ccacggtctt gctccgctgg
     8701 cgagccatcc actaggtctg gtcatgctgg cgctcatctg ctccttcccg ctgctttcac
     8761 ctttcctcgg cccaggcgca gttatcgcac aggttatcgg cgtattgatt ggcgtgcaga
     8821 ttggtctcgg caatattccg ccgcatctgg ctttaccggc actgtttgcc atcaacgcgc
     8881 aggcggcctg cgacttcatc ccggtcggtt tgtcgctggc ggaagcccgt caggacacgg
     8941 ttcgcgtcgg tgtcccttct gtactggtga gccacttttt aaccggcgca ccgactgtac
     9001 tgatcgcctg gtttgtctcc ggttttatct atcaatagag gctgaaacat gaccgttatt
     9061 tatcagacca ccatcacccg tatcggcgcg agtgccactg acgccctcag cgaccagatg
     9121 ctcatcacct ttcgtgaagg cgcgccagcg gacctcgaag agtattgctt cattcattgc
     9181 cacggcgagt tgaaaggtgc actccatccc ggtttgcaat ttacgctcgg gcagcatcgc
     9241 tatccggtga ccgctgttgg cagcgtggcg gaagacaacc ttcgcgaact gggtcatgtc
     9301 accctgcgct tcgatggttt aaacgaagcg gaatttccgg gcactgtcca tgtggcaggc
     9361 cctgtacccg acgatatcgc gccgggatcg gttttgaagt ttgaatctgt taaggagtaa
     9421 aaaatgaatc aggttgccgt tgtcatcggt ggtgggcaaa ccttaggcgc gttcctgtgc
     9481 cacggtctgg ctgccgaggg gtatcgcgtc gcggttgtcg atattcagag cgacaaagcc
     9541 gcaaatgtgg cacaagaaat taacgccgaa tatggtgaag gaacagcgta tagttttggt
     9601 gctgacgcca ccagcgagca aagcgtcctg gcgctctctc gtggggtaga tgaaatcttt
     9661 gggcgcgtgg atttgctggt ctacagcgcc ggaatagcaa aagcagcctt tatcagcgac
     9721 ttccagctcg gcgattttga ccgtgaatct ggtgggttat ttcctgtgtg cccgtgaatt
     9781 ttctcgtttg atgatccgcg acgggattca gggtcgcatt attcagatca actcgaaatc
     9841 cggcaaagtg ggcagcaaac acaactctgg ctacagcgca gcgaaatttg gtggcgtcgg
     9901 gctgactcaa tcactggcgc tggatctggc ggagtacggc attacggtgc attcactgat
     9961 gctcggtaac ctgctgaaat cgcctatgtt ccagtcactg ttaccccaat acgcgaccaa
    10021 gctgggtatc aaaccggatc aagtcgagca gtattacatc gacaaagtac cgctcaaacg
    10081 cggctgcgat tatcaggatg tgctgaatat gctgctgctc tatgccagtc ctaaggcgtc
    10141 gtactgcacc ggacagtcga tcaatgtcac cggcggtcag gtgatgttgt aatcaacagc
    10201 ggagatccgt taaggatctc cgtgagacta atagaatgcc tgatgcgcta cgctcatcag
    10261 gcatacagga cttccgccac tacattaagg aaaagttatg gtatccgcac tcatcaccgt
    10321 cgccgttatc gcctggtgtg cgcaactggc cttaggcggc tggcaaattt ctcgttttaa
    10381 ccgtgccttc gacacactat gccagcaagg gcgggttggc gtgggccgtt ccagcgggcg
    10441 ctttaaacca cgggtcgtgg tcgccatcgc cctggacgat cagcagcgca tcgtcgacac
    10501 cttgtttatg aaaggactga ccgtcttcgc acgaccgcaa aaaattcccg caattaccgg
    10561 tatgcatgcg ggtgatttac agcccgatgt gatctttccc catgatccac tatcacagaa
    10621 tgctctatca ttggcgctta aactgaaacg tggataattt cgttgtgaat gttacttgct
    10681 tgcgaagtta tcattttgaa acctaaatca ggtaatcacg cccatgaaac ctcgtcagcg
    10741 tcaggccgcc attctggagt atctgcaaaa gcagggtaaa tgctcggttg aagaattggc
    10801 gcaatacttt gacaccacag gcacaaccat tcgcaaagat ctggtcattc tggaacatgc
    10861 aggaaccgtc attcgtactt atggcggagt ggtgttgaat aaagaggaat ccgatccgcc
    10921 tatcgatcat aaaacactca tcaacaccca caagaaagag ctgatagcag aagctgccgt
    10981 tagttttatt catgatggcg attcgatcat tcttgatgct ggcagtaccg ttttgcagat
    11041 ggttcccctg ctctcgcgct ttaataacat cacggtgatg accaacagtc tgcatatcgt
    11101 caatgcgcta tctgaactgg ataacgaaca aactatcctg atgccaggcg gaacgtttcg
    11161 taaaaaatcg gcctcatttc acggacagct ggcagagaat gccttcgaac atttcagttt
    11221 cgataaattg tttatgggca ccgacggcat cgatctcaat gcgggcgtaa ccacctttaa
    11281 cgaggtttat accgtcagta aggcaatgtg caatgccgcg cgcgaagtga ttttgatggc
    11341 ggactcatca aagtttggcc gtaaaagccc caacgtagtt tgcagtcttg aaagcgtcga
    11401 taagctgatt accgacgcag gtatcgatcc ggcgtttcgt caggcgctgg aagagaaagg
    11461 gatcgatgtg atcataaccg gagagagcaa tgagtgaagc actactgaac gcgggacgtc
    11521 agacgttaat gctggagttg caggaagcaa gccgtttacc ggaacgtctg ggcgatgatt
    11581 ttgttcgcgc cgccaatatc atcctgcact gcgaaggcaa agtggtggtt tcgggaattg
    11641 gcaaatcggg ccacattggt aagaaaatcg ccgcaacact tgccagtacc ggcacatcgg
    11701 ctttttttgt ccatccggca gaagcactgc acggcgatct ggggatgatt gaaagccgcg
    11761 atgtgatgct gtttatctct tactccggtg gcgcgaagga actggatctg attattccgc
    11821 gtctggaaga taaatctatc gcgctgctgg cgatgaccgg caaaccgacg tcaccgctgg
    11881 gcctggcggc aaaagcggtg ctggatatct ccgtagaacg cgaagcctgc ccgatgcacc
    11941 ttgcgccgac ctccagcacc gtcaataccc tgatgatggg tgacgcgctg gcgatggcgg
    12001 tcatgcaggc gcgcggattt aatgaagaag attttgcccg ctcccaccca gccggggcac
    12061 tgggcgctcg cttgctgaat aaagtgcatc atctgatgcg ccgtgacgat gccatcccac
    12121 aggtggcgtt aaccgccagc gtgatggatg cgatgctgga actcagccgc accggtctgg
    12181 ggctggtggc ggtatgtgac gctcaacaac aggtacaagg cgtctttacc gacggcgatt
    12241 tacgtcgctg gctggtaggc ggcggcgcac tcaccacgcc agttaatgaa gcgatgacga
    12301 caggcggcac cacgttacag gcgcaaagtc gcgccatcga cgccaaagag atcctgatga
    12361 agcgcaaaat cactgccgca ccggtggtgg atgaaaacgg caaactcacc ggcgcaatta
    12421 acctgcagga tttctatcag gccgggatta tttaatcctt cattcccaga cgtttcgcca
    12481 gccgatgcag gttggcgacg tcggtttcca gcatccgcgc gcaggcagcc cagttatgat
    12541 gattttgtgc cagtgcctgg cgaatagttt cacgctggaa cgcttctgtc gcttcacgca
    12601 ggttttgctt aacaacgggc accgccgccg cttctggcgg cggcaacgtc acctcaggaa
    12661 aagcaaaatg ttgcgcctca agaatcactt catcgccgtt gcgggtggct ctcgccagca
    12721 ctaccgcccg atgaatagca tgttccagtt cgcgcacgtt ccccggaaaa cgatagtgtt
    12781 gcagtaaatt tcgcgctccg gcacttaata ccacgcggga gagccccagc cgcaaacgac
    12841 actgctcgca gaaatacccc gccagcagaa tgacatcatc gccccgctca cgcagcggcg
    12901 gcaccgaaag tggaaacacg ctcaggcgat gaaacaagtc agcgcgaaat cgccctgcca
    12961 tcacctcttc gcgtaagtcg cggttagtcg ccgccagcac gcgcacatcg acccgcaaac
    13021 tacggtcatc gccaacgcgc tgaatatcgc catactgcaa caccctcagc agcttggcct
    13081 gcaatgccaa cgacaactcg ccgatctcat ccagaaacag agtgccgtta tccgccattt
    13141 caaacttccc gctgcggtta ctgatagcgc cagtaaacgc tcctttcaca tgcccgaaca
    13201 actcactttc cgccacactt tccggcagtg cagcacagtt gagatagacc agcggattca
    13261 ccgcccgtgg cgaggcttca tgaatcgctt tcgccaccag ctccttaccg gttcccgtct
    13321 caccgctgat caggacgttg agatcggacg ccgccacaat ctcaatctct tttttcagtt
    13381 gcgtcatgcc aggggacaag ccaatcatct gcgtctgttt caccgcttca aacggcgtgg
    13441 catcgcctgg catcatattc tggctttcca gttgttcaat cagcaacgca ttgcttaacg
    13501 ctcccgccgc cagcgcagca atcagccgta gctcttcgtc gctgaaaaca tcgaactgat
    13561 cgggctgcat cccgtcgagc gtcagtgcgc cgatcaggtt ttgcccggca aacaatggca
    13621 gaccaacgca ggcgtgaacc ttcagactct cctgcccagg aatcaaaccg tcatagggat
    13681 cgggcaattc gctgtctgcg ggaaagcgca ccacatcccc ggcgcgggca atcgcttcca
    13741 gccgtggatg cccttccagc gcaaagcgtc taccgagtac atcctttgcc agaccgtcga
    13801 tggcaagcgg aataaactgc cgcgaatcgt aacgtagcaa cgcagacgca tcgcactcca
    13861 gcacctgacg tagcgtggtg atcaggcgct gaaaacgatc ctgatgacca atcccacgct
    13921 gcaattcgat ggcgatattc gccagcacat caacggaaaa actcatcttt gcctcactgt
    13981 caatttgact atagatattg tcatatcgac catttgattg atagtcattt tgactactca
    14041 ttaatgagca taattttatt tatagagtaa aaacaatcag ataaaaaact ggcacgtaat
    14101 ctgcaattag caagacatct ttttagaaca cgctgaataa attgaggttg ctatgtctat
    14161 tgtggtgaaa aataacattc attgggttgg tcaacgtgac tgggaagtgc gtgattttca
    14221 cggtacggaa tataaaacgc tacgcggcag cagttacaac agctacctca tccgcgaaga
    14281 aaaaaacgtg ctgatcgaca ccgtcgacca taaattcagc cgcgaatttg tgcagaacct
    14341 gcgtaatgaa atcgatctgg cggatatcga ttacatcgtg attaaccatg cagaagagga
    14401 ccacgctggg gcgctgaccg aactgatggc gcaaattccc gatacgccga tttactgtac
    14461 tgccaacgct atcgactcga taaatggtca tcatcatcat ccggagtgga attttaatgt
    14521 ggtgaaaact ggtgacacgc tggatatcgg caacggcaaa cagctcattt ttgtcgaaac
    14581 gccaatgctg cactggccgg acagcatgat gacttacctg acaggcgacg cggtgctgtt
    14641 cagtaacgat gctttcggtc agcactactg cgacgagcat ctgttcaacg atgaagtgga
    14701 tcagacggag cttttcgagc agtgccagcg ttactacgcc aatatcctga cgccgttcag
    14761 ccgcctggta acaccgaaaa ttaccgagat cctgggcttt aacttaccag tcgatatgat
    14821 agcgacctct cacggcgtgg tatggcgcga taacccgacg caaattgtcg agctgtacct
    14881 gaaatgggcg gctgattatc aggaagacag aatcaccatt ttctacgaca ccatgtcgaa
    14941 taacacccgc atgatggctg acgctatcgc ccaggggatt gcggaaaccg acccacgcgt
    15001 ggcggtgaaa attttcaacg tcgcccgaag cgataaaaac gaaatcctga ccaatgtctt
    15061 ccgctcaaaa ggcgtgctgg tcggcacttc gacgatgaat aacgtgatga tgccgaaaat
    15121 cgccggtctg gtggaggaga tgaccggatt acgcttccgt aacaaacgcg ccagtgcttt
    15181 cggctctcac ggctggagcg gcggtgcggt ggatcgtctt tccacgcgcc tgcaggatgc
    15241 gggtttcgaa atgtcgctta gcctgaaagc caaatggcga ccagaccagg acgctctgga
    15301 gttatgccgt gaacatggtc gcgaaatcgc ccgtcagtgg gcgctcgcgc cgctgccgca
    15361 gagcacggtg aatacggtgg ttaaagaaga aacctctgcc accacgaagt ccgacctcgg
    15421 cccacggatg cagtgcagcg tctgccagtg gatttacgat ccggcaaaag gcgagccaat
    15481 gcaggacgtt gcgccaggaa cgccgtggag tgaagttccg gataacttcc tctgcccgga
    15541 atgctctctc ggcaaagacg tctttgacga actggcatcg gaggcaaaat gagcaacggc
    15601 attgtgatca ttggttcggg cttcgccgcc cgccaactgg tgaaaaatat tcgcaaacag
    15661 gacgccagta ttccattaac cctgatcgcc gccgacagca tggatgagta caacaaaccg
    15721 gacctcagcc atgttatcag ccaggggcaa cgtgccgacg accttacccg ccagacggcg
    15781 ggtgaatttg ccgggcagtt taatctgcac ctgtttccgc acacctgggt gacggatatc
    15841 gatgccgaag cccatgtggt gaaaagccag aataatcagt ggcaatacga caagctggtg
    15901 ctggcaactg gtgccagtgc ctttgtcccg ccagtgcctg ggcgtgagtt aatgctgacg
    15961 ttaaatagtc agcaagagta tcgcgcctgt gaaacgcaac tgcgggatgc ccgacgcgtg
    16021 ttgattgtcg gcggtggttt gattggtagc gaactggcga tggatttttg tcgtgcaggc
    16081 aaagcggtca cgctaatcga caacgctgcc agtattctgg cgtcattaat gccaccagaa
    16141 gtaagcagcc gcttgcagca tcggttgacg gagatgggcg ttcatctgct gttgaaatct
    16201 cagttgcagg ggctggaaaa aacggattct ggcattctgg caacgctgga ccaccagcgt
    16261 agtatcgaag tggatgcggt aattgccgcc accggactgc gcccggaaac agccctggca
    16321 cgacgcgccg ggctgacgat taatcgcggc gtttgcgtcg atagttatct gcaaaccagt
    16381 aatgacgata tttatgcgct gggcgattgc gcggaaatca acggtcaggt actgccattc
    16441 ctgcagccga ttcaacttag cgcgatggtg ctggcaaaaa atcttctcgg caataacacg
    16501 ccgctgaaac taccggcgat gctggtgaaa atcaaaacgc cggaattacc gctgcatctg
    16561 gcaggcgaaa cccagcgtca ggatttacgc tggcaaatta ataccgaacg ccagggaatg
    16621 gtggctcgcg gtgttgacga tgctgaccag cttcgcgcct ttgtggtcag tgaggatcgg
    16681 atgaaagagg catttggatt gttgaaaaca ttgccgatgt aggtgggcta ctgtgcctaa
    16741 aatgtcggat gcgacgctgg cgcgtcttat ccgacctacg gggacgcatg tgtaggccag
    16801 ataaggcgtt tacgccgcat ccggcaatgg tgtccaaatg caacacgttt tatccgttct
    16861 ggacttcacc cgctaaccaa cgcgccgcag caataacccc ctgccccaga gacaaaccgc
    16921 catcacccgc cggtaaactc tgtggaaaga gcaatgtgaa atcagcgaga taatgcgcca
    16981 gacgtgcacg cagcaaacgg ttatgaataa ccccgccgct aaataccagc gtagtgatac
    17041 cacgcatcgt ggcctgctca cgcatcaacg cggcaaaacc ctgcgccagc gcatcatgaa
    17101 acgcccacgc gcgttgatta accggtgcct gccagttcag ccactgctgc cagaaagcgg
    17161 cgagatccag ttgattgtcc accagcggca tcgtcaccgg atgcgtcact ccgtggcatg
    17221 aggctgcgag cgcctccaga gcacaagccg cttcaccttc ataacttaac gtggctggcg
    17281 cacagcccag tgccgccgcc actgcatcga acaaccgccc acacgatgac gccagcggcg
    17341 cgttaattcc acgctcaatg gcccgcgcca gcacgctcca gttttgctgt tgcacacttg
    17401 ctgtttcagg gtaattctgc cactccggca caaagcgcag gcactgcgcc agcaggtttc
    17461 gccacggctg cttcgctgcc aaatcgccac ccggaagcgc cactgcaggc aagccgccca
    17521 ggtgctcaca ttcgcgatag ttcacccgca ggcactcgcc gccccacaaa gcgccgttct
    17581 cccccatacc gataccgtcg agcgtcaaag caatgacatc accgccatcc aacggccact
    17641 gatgctctgc cagacacgcc gctgcatggg catgatgatg cagtaccgtt tgcgtcggca
    17701 gattcatttc acgcgcccac tggctggaga catagcccgg atgcgcgtca tgcacaacgt
    17761 attgcggggt aaaatcgtag atgttttgca tcaggcgtaa cgcttcgcgc cactgcatct
    17821 ggatgccatc gtcacttaaa tcgcccagat gctgactcaa caccgcttgt tcaccgcgca
    17881 ccaggcagaa ggtatttttc agatccgcgc cgagacacag cacaggcgga acatttttaa
    17941 agcccggagg caaagccagc gcatccggca catacccccg cgaacggcgc agcatttcgc
    18001 cgctttcgcg caccaccgaa tcatccatcc gctgcacgat gtcgcggtta tgtatcaaga
    18061 atccgtcggc aatgccctgc aaatccgcca gcgcctgttc gttgctgata gctggtggtt
    18121 taccgctcag gttgccggag gtcatcacca gcgggcattg cagttcctgt aacagcaaat
    18181 gctggagcgg gttcgcaggc aacattaccc cgacttcgtt aaggtcaggg gcgatatcat
    18241 cacaaagctc aggaacgtat tttttatcca ccagcacaat cggcgcggcg ggcgtggtaa
    18301 gcaactggcg cgcagcgtct ggtaaaccgt cagccactgg caacatgacc gccagcggtt
    18361 tcgccgggcg atgtttgcgc gcccgaagtg tcgccaccgc gttactgtta cgtgcatcgc
    18421 aggcaagatg aaatccgcca atccctttga tggcgacaat gttgcccatt tttaactgtg
    18481 cgatagccgc ctgtaatgcc gcctcttgtt ctgcatgttc accatgactt acccattcaa
    18541 gatgtgggcc acactccggg caggccaccg gctgggcgtg gaagcgacga tcgagcgggt
    18601 cacggtactc tttgtcacaa gccggacaca acggaaacgc cgccatcacg gtaaacgggc
    18661 ggtcgtaagg catggcgcga ataatggtga aacgcgggcc gcagtgggta cagttgataa
    18721 acggataacg ataacgccgt tcgcctgggg tattcatttc ggcaaggcaa gcagggcaag
    18781 tagcggcatc cgggacaatt tgcgtattca tggcacctcc cgcgctctgg cggatggtga
    18841 actcagtggg cagttgtgac cagatgtacg gctcacgctc gacgctatca atacgcgcca
    18901 gcggcgggca gtgctgatgc aattgaacaa gtaacgtttc cgggtcttcc cgcagccgga
    18961 cttctacgcc atcgccgtca ttacagacat cgccgtgaag atttaattgc tgtgccagct
    19021 gccagacaaa cggacgaaaa ccgacgccct gcactttgcc acgaatacgc agttggacac
    19081 cgcaagatgt gttttttgcc attgagttat tcccgccatc atgaattgcg taacccgtcc
    19141 tgccggacac gactgcgtcg tatccggcag tctcaggtcg gcgataccgc cagctccgta
    19201 ttctacgaat atttccggga attcctttga tgccagaaca gttctgtaag atttttagaa
    19261 catcagcgcc gtacggcggc gtttttctgc actcagttgt tcaagtttat tacgatcgac
    19321 acaaatcagc gcatgagtcg ggcaagccgc catacacgcc gggccgtctt cacgatggtt
    19381 gcacaggtca catttattgg cttcggcttt gtcagcccgt acattcagcc ccgcgccgct
    19441 gttgcgaatc accggacgta ccaccacttc catcgcgcca tacgggcaag ccacaacgca
    19501 ggttttgcaa ccaatgcaac gttcctgcat cacatgaaca aaccctttat cacggctgat
    19561 agcaccattc gggcagacgt tagcgcacgg tgcatcttca cactgacggc aaactgtcgc
    19621 cgtggaaatg ttcacacctt taatgacatg gatacgcggt aaaaatgttt ccggggtcag
    19681 cgatgcacag tcctgatttt cctgatgaga aaccacgcac gccacttcac aggtacagca
    19741 accaatacat ttactcgcgt cagcaatgat gaaacggttc atcaaattct ccagcaatga
    19801 cagttaatgc gccgatacat tcacaaatca tgccagtttt aattcactgt tatttaagga
    19861 aattaatttc tgtaatgcag gaaaaaagat gtcatcgaca ctagtgacga tgacatgtga
    19921 tgacaatgtt tatcgcgaaa gagcaatgag tgagtcgcgg cggatcagtt ttccgctgaa
    19981 ggttttcggc ggtgagaaat ccccgccatc gagcataaaa atcagccgtc caataatttc
    20041 ctgaatcatc tcagtcaccg gaatttttac gctggagagc gccggaacga tatagggggc
    20101 aattgcgata tcatcgaatc cgataactga cacctgctct ggcaccgcta cgccgcgctc
    20161 gtgcaacgct ttcatcgcac ctatcgccat atcgtcgtta ctggcaacta acgcgctaaa
    20221 tttagcccca cgttcgagca acgtttctac cccttcggcc ccgctggcgg gcgtccattt
    20281 accgttagcg ataagttttt cattgagcgc aataccatgc tgcgccagcg cgtctttata
    20341 cccagcaaga cgttcaatgc tggtggggga atccatcgag ccggtaagga aagcaatctc
    20401 ctgatgtccg gcgtttatca actctgccac ggcattaaag ctggtctgtt tatgatcgca
    20461 ccagacgcta tggctgctgt ttttgcgcag gcggcgatta agcaccatta tcggctgact
    20521 gtgcgcgtca atgatgtcat cgatctcatc cacgcttaaa aaacgcgggt aaatcataat
    20581 ggcgtcgcag cgcaaatcca gcagatactg aatcgcctgg cgctcttctt cggcgctgtg
    20641 tttaccatct gccaatagca actgccgccc tttctcttcc gccattcgcg cggcatgaaa
    20701 gagtaattca ctaaaataaa tgccgtggta aagcgtgttg gtcactacca accccagcgt
    20761 ctgagtactc ttcgccgaca gattgcgcgc cagcaagttt gggcggtaac cgctctcttc
    20821 taccgcctga aacacgcgat ctttagtctc ctggctgacg tagccattac ctgaaagcac
    20881 gcgggaaacg gtcgcttttg aaaccccggc gcgcttcgcc acttccagca tcgtcgtcat
    20941 cattttcatc cctttacacg caatcaacgc agtgtactgc accgtttgcc gattgtcctt
    21001 gcgcaatcgg cgggaaaaat attcaggtga ccggtttcac aaatataaaa aatgaacaat
    21061 taactctctt gcttatttag tgacaactat tcatgatttt gtgaaaccgg tttcctaatt
    21121 ccgtttcagc atcggcattt ttccgttacg tcgactgata acaactacat ctaccctact
    21181 gataacagga taaaatccga tggccaaaaa ttatgcggcg ctggcacgct cggtgatagc
    21241 ggcactgggc ggcgttgata acatctcggc ggtcacgcac tgtatgacgc gtttgcgcgt
    21301 tgttatcaaa gatgaccagc ttatcgacag cccgacgtta aaaaccatcc ccggcgtgct
    21361 cggcgtggta cgtagtgaca accagtgtca ggtgattatc ggcaataccg tttcacaagc
    21421 ctttcaggaa gtcgtcagcc tgctgccggg agatatgcag cccgcacagc ccgtgggtaa
    21481 acccaaactc acgctgcgtc gcattggtgc ggggatcctc gatgcgctga tcggcaccat
    21541 gtcaccgctg atcccggcga ttatcggcgg atcgatggtc aaactgctgg cgatgatcct
    21601 cgagatgagc ggcgtgctga caaaaggatc gccgacctta accattctga atgtgattgg
    21661 tgacggtgct ttcttcttcc tgccgctgat ggtcgcggca tctgccgcca tcaaatttaa
    21721 aaccaatatg tcgctggcga ttgccatcgc gggtgtgctg gtacatccga gttttattga
    21781 actgatggcg aaagcggccc agggtgaaca tgtcgaattt gccctgattc cggtcaccgc
    21841 ggtgaaatac acctacacag tgatcccggc gctggtgatg acctggtgcc tgtcatatat
    21901 cgaacgctgg gtggatcgca ttacaccggc ggtgaccaaa aacttcctca agccaatgct
    21961 gattgtgttg attgccgcac cgctggcaat cctgctgatt ggcccgattg gtatctggat
    22021 cggtagcgcc atttcggcgc tggtttacac cattcatggt tatctgggct ggctttcagt
    22081 cgccattatg ggcgcgctgt ggcctctgct ggtaatgacc gggatgcacc gcgtctttac
    22141 gccaaccatc attcagacca ttgccgaaac cggcaaagaa gggatggtca tgccgtcaga
    22201 gatcggcgct aacctgtcgc tgggcggttc atcactggcg gtggcgtgga aaacgaaaaa
    22261 cccggaactg cgccagacgg cgctggctgc ggcggcatca gccattatgg cggggatttc
    22321 cgaaccggcg ttatacggcg tggcgatccg cctgaaacgt ccgcttatcg ccagtcttat
    22381 cagcggtttt atttgcggcg cggttgccgg tatggcgggg cttgccagcc actcaatggc
    22441 agcgccaggg ttatttacca gcgtgcaatt cttcgatccg gcgaatccaa tgagcatcgt
    22501 ctgggtgttc gcggtcatgg cgctggcggt ggtgctgtcg tttatcctca cactgttgct
    22561 cggctttgag gatattcctg ttgaggaagc ggctgccgag gcgcgaaagc atcagagcgt
    22621 acaaccgacc gtcgccaaag aagtaagtct taattgagga tgaaaatgtc agtatttcca
    22681 gaaggttttt tatggggcgg cgcgcttgcc gccaaccagt ctgaaggtgc gttccgtgaa
    22741 ggtggcaaag ggctgaccac tgtcgatatg atcccacacg gcgagcatcg aatggcggtg
    22801 aaactggggc tggaaaaacg ttttcagttg cgcgatgacg agttttatcc cagccatgag
    22861 gcgacggatt tttatcatcg ttataaagaa gatatcgccc tgatggcaga gatgggattc
    22921 aaggttttcc gtacctcaat tgcctggagc cgtctctttc cgcagggcga tgaaatcacg
    22981 cccaatcagc agggcattgc tttttatcgt tctgtctttg aagagtgtaa aaagtacggt
    23041 atcgaaccgc tggtcacgtt gtgccacttc gatgtgccga tgcatctggt caccgaatat
    23101 ggctcctggc gtaaccgcaa gctggtggag tttttcagcc gctacgcccg gacctgcttt
    23161 gaagcatttg atggtctggt gaaatactgg cttaccttca atgaaatcaa cattatgttg
    23221 catagcccgt tctccggcgc gggtctggtg tttgaagaag gtgaaaatca ggatcaggtg
    23281 aaatatcagg ccgcgcatca ccagctggtt gccagtgcgc tagccaccaa aatcgcccat
    23341 gaggttaacc cgcaaaatca ggtgggctgt atgctggcgg gcggtaactt ctacccttac
    23401 agttgcaagc cggaagatgt ctgggcggcg ctggagaaag accgggaaaa cctgtttttt
    23461 atcgatgtgc aggcgcgggg cacgtatccg gcttactctg cccgcgtatt ccgcgaaaaa
    23521 ggggtaacca tcgacaaagc accgggcgat gatgaaatcc tgaaaaacac cgtcgatttt
    23581 gtctctttca gctattacgc ctcgcgctgc gcctcggcgg agatgaacgc caacaatagc
    23641 agtgcggcga acgtagtgaa atcgctgcgt aacccgtatc tgcaagtgag cgactggggc
    23701 tggggtattg atccactcgg tctgcgtatc accatgaaca tgatgtacga ccgttatcag
    23761 aagccgctgt ttctggtgga aaacggcctc ggcgcaaaag atgaacttgc tgccaatggc
    23821 gagattaacg atgactatcg catcagctat ttacgcgaac atatccgcgc aatgggcgaa
    23881 gcgattgcag atggcattcc gctgatgggc tacaccacct ggggctgtat tgatttagtc
    23941 tccgcctcta cgggtgaaat gagcaaacgc tacggttttg tctacgtaga ccgtgacgac
    24001 gcaggcaacg gcacgctgac gcgcacacgt aagaaatcgt tctggtggta taaaaaagtg
    24061 attgccagta atggggaaga tttagagtag gtaacagtgc cggatgcggc gtgaacgcct
    24121 tatccggcct acatgtcgca cgaaccagga aggcccgata agcgcagcgc atcgggcaat
    24181 ctggggtttg gcatcagtca aaatcaaaga aaccattccg gcttaacgcc aaatcgggct
    24241 gaaagtgcct taatgtggga tatcgtcagg gatcgttgtc cagacaaaat ctgactgacc
    24301 agggatttag agccgatttc gttcttcaaa tccgcatagg tcagattatg ttgatcaatc
    24361 agcgtgcgaa gtaacgccac gccaacaggc attgccgcaa cggctttgtc gaactctgca
    24421 aactcttggt tgttgttttc atattctgca atgcggctcg ccagaaagtc gataagtgga
    24481 ttggtgtcat cttcttcaat caaatattcg accagttcca gtgcttcacg ataatcatct
    24541 tctgagtcac tacctcccag aaaggggact gcattgacca gttctcttgt tgctttaaca
    24601 gcgcgtgcag cattagcagt cattctttgt tctctctgta gtaacgtgtc agtttgtcat
    24661 actcggcgtg attagttatg tgcttcacaa agaaccgctt attaacaaag tttatgtagg
    24721 caatcaccct taggttgttc ccacctacat ccagaacgta ccatttgttg cgatatttaa
    24781 aattgtcgag gttgggaaac gccgttcgca tctcttctgg cgtagaaaaa tccgtttcct
    24841 taataacccg atacaatgcc tgaagagcca gagcgtcgtt aggatattta cgcgcagact
    24901 cttcaaatgg cgctttagaa atgatgtgca tttttaagtt ttaacttcct tatagtaagt
    24961 aaacagccac atctgcgagt tctatcctta tgtgttcgga aactatagct catcaaatcc
    25021 aagttcacaa tatgtaaaca cttgagcgcg atataattac ccccaaaatc ctcatagcca
    25081 ctctgcaaaa agtcagttct ggttcacccc atcaagaaca tcccccttta tcaagcaacg
    25141 ctgcgtctgc gccagatctt ccgtttgcgg gtcgcgcccg gcgatgggtt tgaggacgag
    25201 tttacggttg ggggtataag taaaacgcgt gagcgcggga tagcttaact tgccaaacca
    25261 gccgtgaaca cgctgccatt tggcgtcttt tacctgatac agactggtta tccagtaacc
    25321 ggaatcaaac tgcatatcca gcaattgcgt gtgaccattg ccttgcaaat ccagcaaatc
    25381 atctacgcca gttttgcttg cggtgtagaa tcccctgggt tcaaagacac agggacggcc
    25441 ttcgcgcgtg aacgtcataa gaattaaatg tgcataagga gccagaccat taccggtact
    25501 tggtaaccag ataaccagat cctgaatgcc gttacgatca agatcggcgc gatagagttg
    25561 ccctttgccc gctatgtttg ccagcatacc cacaggcaca gaccaggatt taccggcgcg
    25621 atctttaccg ttcaattgcc attcactatc atcatttggc atcttttcaa gcgtcacgcc
    25681 agcaacgccc acttttctaa aatgccaggt ttgctcggca tcataaggca tggtcaggag
    25741 aggtttcagc gcccgctttt gcgcgtattc gctgtcactc attggcagat attcggtaaa
    25801 gctggtggcc tgtgcaggca gcactaagct caataaaaaa gcgcacatcc ctgttctgat
    25861 attcatttcg ctgaattccc tgatggagaa ttattcttct tccaccgcta actgcgcgaa
    25921 gccgccattc ccttcccagc cttccagtcg ttgataaacg gtttctaccg catctttaat
    25981 cggctgagtc atcgggtagt aaaagccgac gatatccggc tggatgccga ggaaaatcac
    26041 ttcgccaatg tcttctttca actggtcgat aagatagttc agcggcatgt tatgggtagt
    26101 catcataaac atctcggcga tatcatccgg gtcgatgatg cggatttcgc cggggtttag
    26161 ccccatatca gtagcgtcaa caatcagtaa ccgcgtaggg cgcagttcac ggatggcgac
    26221 gatgtcgttt tccggtgcac taccgccatc aatcaccacc cagttacctt tcggcgcggc
    26281 ggcgcacttt tccgccagca gcgggccagc gccatcatcg cccatcatgc tattgccaac
    26341 acagagtaaa acgtcagtca cgcagtctcc tcaccatcaa atagatggcg ttttcctgat
    26401 gaatatcatg cagcatactt aacagcattg tactccattc cttctgctgc gaagtttgcg
    26461 ctgcccgggc tttatcgaat gcgttggcca gcatcggcac atggttaatg tcgatgacga
    26521 tctcaccata tttcggcacg ccttccattt tgcggcgggc ttcactgccc gcctccagag
    26581 tggcaatcca tgccagatat tcatcccacg ggcaggtgag cgccgcttcc aggcaatcga
    26641 taaccccaag gtggtgacca atcgccaggc tgtaatagac cacctgctgc gcctcggcgg
    26701 gcgtggcatc gttctcatca ataaatttac ggctcagttg actgaacacc accttttcac
    26761 tcatcggata cgcgcctctt caacaacatg attcagatgg ctgacaatct cgttcagacg
    26821 cggatcgttt tccgcttcca gccagcgtgc aacctgttct tcgccctgcc ctaactgtgt
    26881 caggaagtca tcggcaatct gacgaccgta acgataaccc gccaggcggc gtgcttcgcg
    26941 atccactttc acacgcagcg gctgcaccat atcgccatgc aggatttccg cctgttgttc
    27001 atcctgctca cccgggccac gggcgtgaat tttctgctcc agcaggccga gcgccattgc
    27061 aaagccgtac agcgtggcgg caggcgttgg cgggcaacca ggaatgtaga catctaccgg
    27121 gacgatttta tccgtaccgc cccacacgca gtagagatcg tggaaaatac caccgctgtt
    27181 accgcaggca ccgtaggaga tacaaatttt cgggtccggc gcggactgcc acgcacgcag
    27241 cgcaggggat cgcattgcac gggtgaccgc gccggtaaac agtaaaatat ccgcatgacg
    27301 cggtgaagga acgactttaa tgccgaagcg ttctgcatca aacagcggcg aaagcgtggc
    27361 gaaaatttcg atttcgcagc cgttgcagcc gccgcagtcc acgcgataaa cgtaggcaga
    27421 acgtttgatt tttttcagta acgacgcctt catgctggcg atggattcat ccaccgtcat
    27481 cgggaccgga atgccgttgg cgtcacgggg gcctaataaa ttgctcatca gatggcctct
    27541 ttcatatggc gagtcagttc aatacggtcg gacggcacca ggcatttctg gcgcttacat
    27601 tccgggcaag tctcaaagct ttcgcggtgg ttttccgcgc ggctgtcgcc gttgtgctta
    27661 agcagcgcaa tggcgtagtc gatctctttc tggacggcga aaggacgatt gcagacgcgg
    27721 cagttgcaca gcgcgaagcg ggactgttgc aggaagtctt ctttcttcca cactgccagt
    27781 tcgtactctt gcgacagttt gatcgccgcc gtcgggcaaa cttcttcgca gcgtccacag
    27841 aagatgcagc gcccgagatt aaactcccac gccagctcac cggtggcgag gtcagtttca
    27901 accgttaagg cgtttgacgg gcaggcattg acgcaggccg cacagccgat gcactgctgc
    27961 gggttctgct ccggcttacc acggaagttt ttatcaaccg caatcggctc cagcggataa
    28021 gacgaggtcg ccgtgccggt tttgatgact tttttgataa aggtaaacat ggcgattcct
    28081 tatttcagcg gcgagttttt acgctcaatg ctgtaacgct cgagttcttt gtacggcacc
    28141 actttgctct ttttcttacg cacatcgacc acggtcatgc ggtcggtaca ggagtagcaa
    28201 gggtccaagc taccgataat cagcggcgca tcggaaacgg tgttaccgcg cagcatgtag
    28261 cgcagggtcg gccagttcgc gtaggtcgcg gcacggcagc gccagcggta cagcttctgg
    28321 ttgtcgccgg tcatgctcca gtggatatcg tcgccgcgcg gcgcttcggc aaagcccagc
    28381 gcgaaacggt gcggaatgta ggtaaagcct tccaccatca gcggaccgcc cggcaggtta
    28441 tccagaccgt agtcgatcat gttcagcgcg gtataaactt cgttgatacg cactttcaga
    28501 cgggaaataa cgtcgcagcc ctgctcgctg tggacttcca ttggcagcag gccataaccg
    28561 acaaacgggt gatcggcgcg agtatcgcgg gcgtgaccgc tggcgcgaac catcgggcca
    28621 acgttactga aatcgcgggc catttccggg tccagacgac caatgccgac ggtgcgctgt
    28681 tccatgttcg gcgtactcag cagcacatcc accagctcct gcacttcacg acgcatctgt
    28741 tgcgccagct ggcgggtctg gatcatgtca tctttcaaca gatcgcgacg aatcccgccg
    28801 atcaggttca ggccgtaggt tttacgcgca ccggtgagga tctctgccat tttcatggag
    28861 gtttcacgca cgcggaagaa ctgcataaag ccggagtcaa agccggtaaa gtggcaggcg
    28921 agaccgaggt tgagcagatg cgagtgcagg cgttccacct ccagcagaat ggcgcggatc
    28981 atctgcgcac gttctggcac ctgaataccc atcgcgtttt ctaccgacgt ggtgtaggcg
    29041 gtgctgtggg caaagccgca gatcccgcac acacggtcag agaggaaggt cacttcgttg
    29101 taacccatac gggtttccgc cagtttttcc atgccgcgat gaacgtagaa cagacggtag
    29161 tcggcgtcga taatgttttc gccatcgacg aacagacgga agtggcccgg ttcgtcagaa
    29221 gtgacgtgca gcggaccaat cggcacgacg ttgtttttct tgtcgcccag ttcgttgatg
    29281 aactcgtagg tttcagcatc ggtggtcggt gccggacgct gacgataatc catgctgtct
    29341 ttacgcagcg gataaagttc atccggcagc accagacggc gttcatccgg cagaccaacc
    29401 ggaatcaaac cgtacatatc gcgcacttcg cgctcgcccc acaccgccgc cggaacgcgc
    29461 ggcgtcacgg acggatactc cggtttgttg gcatcaactt cgacgcgaac cgttacccag
    29521 cacttagtcc ccttctccat cgacagcacg taataaacgg cgtaatgacc attcagtttg
    29581 cgttcgtcgt taccaaaaag caccgacagc cagcccccct gcttgtagta gagaaactcc
    29641 accacttccg gcagatagtt cacctttacg gtgacagtca gctgatcttt ggtctgccag
    29701 gcgtggtcca gcacgacgcc cggaaatgcc tcattcagcg cggcgagata atgttgacct
    29761 aatttttctt cagacatgct caaactctct ttaatcacgc cgccagcaag gagacgaacg
    29821 ctaaaaatgc aaagccaaac ccagtccagg taatgcgcgg agtaatatca agacgcagac
    29881 gggccatgct gttttcgaac agcgcgataa ccaggacgcc gacgaccagt ttgacgatgg
    29941 caatcaccag cgccagcagc agcccaccgg cggtgaaggt ttccatctgt ccccacggga
    30001 taaatacgcc gacgaacatc tgcagcacca ccagctgttt caggctgata ccccatttca
    30061 tgacgccaaa gccgctgccg ctgtattcag agagcgggcc ttcctgcagc tcctgctcgg
    30121 cttccgccag gtcgaacggc agtttgccca tttcgataaa ggtggcgaac gcacaggcac
    30181 aaagcgccag taccagcggg atgctctggc tcagcggcca gtgataaacg gtgtcggtga
    30241 tgttgctgat gttggtggaa ccggcaacct gtgcggcaac ccacagacca agcagcagca
    30301 tcggttcgac cagcacgcca agcatcgctt cacggctcgc gccgatagcg gtaaacgggc
    30361 taccggtatc cagaccagaa atggcaaaga agaaacgcgc gatggcaaag agatacagta
    30421 aggtgattaa atcacccagt tgcggcagcg gagaaccgac ggtcaccacc ggcagcgcag
    30481 tagcgatagt cagcatgacg cccaccatca catacggcgt caggcggaac acccagccgg
    30541 aggcatccgg gccaacgctc tggcggccca gcagtttgaa aatgtcacga tactcctgca
    30601 acacacccgg cccacgacgg ttatgcagac gggcgcgcgc cacgcgggta ataccggaga
    30661 gcagcggcgc aacggcaaat aacaccagcg cctgaattaa cggatataaa acactcattc
    30721 tcaggctcct cgtgaaacaa taatcaccac cagtaccgcc agttcaacca gcgccatccg
    30781 gcggaacagc aacgcactac cttcgcactg ccagcccggc accagcgaca ccggattcag
    30841 ccatttgcgc agtttcagca ctggcgcaaa cgcctgttta accggcatgg caaaaccgtg
    30901 agcggtaatc accattgatt tttcatggtc gtagtcgcac acccaggccg caccgcggga
    30961 acgtgacggc aggcggtcgc ctttgcaaat cgccataatg atgaatggca gcagcgggca
    31021 ggcaatcagc agcaatgtga tcattggttg agaaacggtg gtgttagcag gctccagagg
    31081 cagaggtaca gcagcagaga gcatcggcag cagccacggc gcagcaacac cgccaattac
    31141 gcagcaaatc gccagtgcca ctacgcttac gctcatcagg agcggcgcac aggtggcgtt
    31201 ttcggcttct ttggtacgcg gcgcgccgag gaacgtgacg ccatagactt tcgccataca
    31261 catcaccgcc agcgcaccgg taattgccag acccacagcg agcagcggcc ccagcagacg
    31321 ggcaacaaac gcgccgctat tgctcagttt gaagaaggat tgatagataa cccattcccc
    31381 ggcaaacccg ttcagtggcg gcagcgcagc cattgccatc aacccgacta acatggcgat
    31441 ggagataacc ggcattttct tgccaatacc accgagtttt tcgatatcgc gatgaccggt
    31501 acggaaccag acgctccccg ccccgaggaa cagtacgctt ttgaacaggc tatggttaag
    31561 cagatggtac agaccaccga ccaggccaag agcaatcagc gccggttgtt cgagcgcgat
    31621 acccgttacg ccagcgccca gccccagcag gataatgccg atattttcca gggtgtggta
    31681 agccagcagg cgctggatgt tgtgttccat caacgcatac agaccaccga cgaacgcggt
    31741 gatcatgccg agcaccagca gcgcgatccc ccaccacagc ggtgcattac cgcccagcag
    31801 tgacagggtt aacatgccca gcaggccaat tttcatgact accgtagaaa acaacgcggc
    31861 agctggcgca gaggcgttag catgtgcctg cggcacccag ccgtgcagcg gaataatccc
    31921 ggccagcagg ccaaagccaa tcacaccgag cagccagata tcggaaccga gcggcagctg
    31981 ttgcatacgc atatccagta ggcacagatc cagcgtgcca taacgctgcc acagcagcca
    32041 gcaagcaatc accagcagca gagtgccaag acgccccagc gcaaaccaca gtttgccctc
    32101 tttgctgttg ctggtgagga acaccgcaca caaggccatg acttcggcca ttaccacgaa
    32161 catgccgagg ttgctggcga tgacggcgca gacggcggca gccatcaaca tattgatctg
    32221 caagccgttg cacttcacct gcgcgtggcg atgccagtca atgttgtaga ggctgacaaa
    32281 cagaccgcac agaccgagcg taatcagcca aatcgcgtta agcggagaga tttgcacatc
    32341 gtagcttacc agcgacagcg caccgctcac gccaaccgcg ccagtcagta cagtgaagcc
    32401 cgcggctgcc gtatacagac taccaaccgc gccgccaatt ccagctatcc agccacttaa
    32461 cgctttttga aaagaaaaga gaaatgccag aacagcggcg gcgacaaacc atgccacgcc
    32521 gctattgatc agggaaattg cgctcattta gcctctccac tttgagcctg ctgaaacaag
    32581 gtgagatcgc caaagtccgt gttaaaggtc agctcacgct tacgtttgct gacgcgggcg
    32641 atatcggtgt tatccaccag atgcagggct ttagtcgggc acatccgcac gcaggccgga
    32701 ccttgttcat caaaactgca aaggtcacat ttgacggcga tcgcgcgaat acccggcacc
    32761 cagtcaagca gcgtactgat acgcgccgga gcaggcggtg ccggtggcgc tttcggggta
    32821 ttggcgtttg ccggaatatc cagcggacgg ctgccggaaa attcaattgc gccaaacggg
    32881 caggcgatgc cgcacagttt gcagcttacg cacagacttt cattcaactg cacggcccca
    32941 tcaatgcggg tgatggcgtt aaccgggcaa accaccgcac agggtgcatc ttcacagtgg
    33001 tgacagagct gcggcgcaga ttctttttca ttcagcatca ctctcaggcg cggcattgat
    33061 tgcaggccgt gctggcgatg cgtctctgaa caggcggcct cacaagtgtg gcagccgata
    33121 cagagcgtgg agtcaacaat tacaaaacga ttcaccaggc attcctcagg tgattgtcat
    33181 ttttgacgaa aacatgccga tgaaatgtca ttttcgacac tcatcgacac gcccatcccc
    33241 aaacaggcgt aacgcctgca aaacgggcaa agcctcagct catgctgccg ggctttgtcc
    33301 ctttaccagt tggcttaaat tcaccggcac attgttttca atagcggtgt ataagctgtc
    33361 gtaaacgcca gcgatttttt cgagatagtg ttccagaacc tgttcgcgat cgcggatgtt
    33421 gaccttgcca tcaatcatgc cgtcaatgct cagttgcgcc tgcgcaatcc gctgtttgtc
    33481 ttcgccatct tttgtttcga cgtaatactc gatggtgtgg ctgttaaagc catccgccag
    33541 ggtgacgtaa atacgaaaat gttcaaaaag gacgaaacag agttctttgt ccggcgcgca
    33601 gctcatggcg tgatgcaggc gcgctttcga taacgtgatc ccctgaacca gcgaattgca
    33661 gtagatgtgc cactggtcct gtaggcgaca atgccgctgt gcgatgtaat cggctttctc
    33721 gcttatttcc caaatagtca ttgtcaggtt acccgtttaa cagagatgcc agctttaagc
    33781 attttctatg ccaactttta atttattgtt attaaataga tttttaagct aaagatgaat
    33841 ttcgtcgccg tgtcgacgtg tcatttcgac atcatcgaca ttattcaccg cagggataat
    33901 caacactggc acaattattg cttgtagctg gcaatagtta atgggaggcg atatgcacga
    33961 aataaccctc tgccaacggg cactggaatt gatcgaacag caggccgcaa aacacggcgc
    34021 aaaacgcgta actggggtct ggctcaaaat tggcgcattt tcttgtgtcg aaaccagctc
    34081 tcttgccttt tgttttgatc tggtttgccg cggcagcgtg gcggaaggtt gtaaactgca
    34141 cctcgaagaa caagaggccg aatgctggtg tgaaacatgc caacagtatg tgacgctact
    34201 gacccagcgc gtccgccgct gtccacagtg tcatggtgac atgctacaga ttgtggcaga
    34261 cgacggttta cagattcggc ggatagaaat agaccaggag tgagcgatgt gtacaacatg
    34321 cggttgcggt gaaggcaacc tgtatatcga gggtgatgaa cataaccctc attccgcgtt
    34381 tcgtagcgcg ccatttgccc cggcggcacg cccgaagatg aaaatcaccg gcattaaagc
    34441 gcctgaattt acccccagcc agactgaaga aggcgacctg cattacggtc atggcgaagc
    34501 gggcactcac gcgccgggca tgagccagcg tcggatgctg gaagtcgaaa ttgacgtgct
    34561 ggacaaaaat aaccgtctgg ctgaacgcaa ccgcgcgcgc tttgctgccc gcaagcaact
    34621 ggtgctcaac ctggtttcca gccctggttc cggtaaaacc accctgctga cggaaaccct
    34681 aatgcgcctg aaagacagcg ttccgtgcgc ggttattgaa ggtgaccagc aaaccgtgaa
    34741 cgatgccgca cgcattcgcg ctaccggcac accagcgatt caggtgaaca ccggtaaagg
    34801 ctgccatctt gacgcacaga tgattgccga cgccgcaccg cgtctgccac tggacgataa
    34861 cggtattctg tttatcgaaa acgttggcaa cctcgtatgc ccggccagct tcgatctcgg
    34921 tgaaaaacac aaagtggcgg tgctttccgt taccgaaggt gaagacaaac cgctgaaata
    34981 tccgcatatg tttgccgccg cctcgctgat gctgctcaac aaagttgacc tgttgccgta
    35041 tctcaacttt gacgttgaga agtgcatcgc ctgcgcccgc gaagtcaatc cagaaattga
    35101 aatcatcctt atttccgcca ccagcggcga agggatggac cagtggctga actggctgga
    35161 gacacagcga tgtgcatagg cgttcccggc cagatccgca ccattgacgg caaccaggca
    35221 aaagtcgacg tctgcggcat tcagcgcgat gtcgatttaa cgttagtcgg cagctgcgat
    35281 gaaaacggtc agccgcgcgt gggccagtgg gtactggtac acgttggctt tgccatgagc
    35341 gtaattaatg aagccgaagc acgcgacact ctcgacgcct tacaaaacat gtttgacgtt
    35401 gagccggatg tcggcgcgct gttgtatggc gaggaaaaat aatgcgtttt gttgatgaat
    35461 atcgcgcgcc ggaacaggtg atgcagttaa ttgagcatct gcgcgaacgt gcttcacatc
    35521 tctcttacac cgccgaacgc cctctgcgga ttatggaagt gtgcggtggt catacccacg
    35581 ccatttttaa attcggcctc gaccagttac tgcctgaaaa cgttgagttt atccacggtc
    35641 cgggttgccc ggtgtgcgta ctgccgatgg gtagaatcga cacctgcgtg gagattgcca
    35701 gccatccgga agtcatcttc tgtacctttg gcgacgccat gcgcgtgccg gggaaacagg
    35761 gatcgctgtt gcaggcaaaa gcacgcggtg ccgatgtgcg catcgtttac tcgccgatgg
    35821 atgcgttgaa actggcgcag gagaatccaa cccgtaaagt cgtattcttc ggcttaggtt
    35881 ttgaaaccac catgccgacc accgccatca ccctgcaaca ggcgaaagcc cgcgatgtgc
    35941 agaattttta cttcttctgc cagcatatta cgcttatccc gacgctgcgc agtttgctgg
    36001 aacagccgga taacggtatc gacgcgttcc tcgcgccggg ccacgttagt atggttatcg
    36061 gcactgatgc ctataatttt atcgccagcg attttcatcg tccgctggtg gtcgctggtt
    36121 tcgaacccct tgatctactg caaggcgtgg tcatgctggt ggagcagaaa atagcggccc
    36181 acagcaaggt agagaatcag tatcgtcggg tggtaccgga tgccggtaac ctgctggcgc
    36241 aacaggcaat tgctgatgtg ttctgtgtca acggcgacag cgaatggcgc ggcttaggcg
    36301 tgattgaatc ttctggcgtg cacctgacgc cggattatca acgattcgat gccgaagcac
    36361 atttccgccc agcaccgcag caggtctgcg atgacccgcg cgcgcgttgt ggtgaggtat
    36421 taacgggcaa atgtaagccg catcaatgcc cgctgtttgg taacacctgt aatcctcaaa
    36481 ccgcatttgg tgcactgatg gtctcctccg aaggagcgtg cgccgcgtgg tatcagtatc
    36541 gtcagcagga gaatgaagcg tgaataatat ccaactcgcc cacggcagcg gcggccaggc
    36601 gatgcagcaa ttaatcaaca gcctgtttat ggaagccttt gccaacccgt ggctggcaga
    36661 gcaggaagac caggcacgtc ttgatctggc gcagctggta gcagaaggcg accgtctggc
    36721 gttctccacc gacagttacg tcattgaccc gctgttcttt cctggcggta atatcggcaa
    36781 gctggcgatt tgcggcacag ccaatgacgt tgcggtcagt ggcgctattc cgcgctatct
    36841 ctcctgtggc tttatcctcg aagaaggatt gccgatggag acactgaaag ccgtagtgac
    36901 cagcatggca gaaaccgcgc gcgcggcagg cattgccatc gttactggcg atactaaagt
    36961 ggtgcagcgc ggcgcggcag ataaactgtt tatcaacacc gctggcatgg gcgcaattcc
    37021 ggcgaatatt cactggggcg cacagacgct aaccgcaggc gatgtattac tggttagcgg
    37081 cacgctcggg gaccacgggg cgactatcct taacctgcgt gagcagctgg ggctggatgg
    37141 cgaactggtc agcgactgcg cggtactgac gccgcttatt cagacgctgc gtgacattcc
    37201 cggcgtgaaa gcgctgcgtg atgccacccg tggtggtgtg aacgcggtag ttcatgagtt
    37261 cgcggcagcc tgcggttgtg gtattgaaat ttcagaagtg gcactgccgg ttaaacctgc
    37321 cgtgcgcggc gtttgcgaac tgctgggact ggacgccctt aactttgcca acgaaggcaa
    37381 actggtgatc gccgttgaac gcaacgcggc agagcaagtg ctggcagcgt tacattccca
    37441 tccactgggg aaagacgcgg cgctaattgg tgaagtggtg gaacgtaaag gtgttcgtct
    37501 tgccggtctg tatggtgtga aacgaaccct cgatttacca cacgccgaac ctcttccgcg
    37561 tatatgctaa taaaattcta aatctcctat agttagtcaa tgaccttttg caccgctttg
    37621 cggtgctttc ctggaagaac aaaatgtcat atacaccgat gagtgatctc ggacagcaag
    37681 ggttgttcga catcactcgg acactattgc agcagcccga tctggcctcg ctgtgtgagg
    37741 ctctttcgca actggtaaag cgttctgcgc tcgccgacaa cgcggctatt gtgttgtggc
    37801 aagcgcagac tcaacgtgcg tcttattacg catcgcgtga aaaagacacc ccattaaata
    37861 tgaagacgaa actgttctgg cacacggtcc ggtacgccgc attttgtcgc gccctgatac
    37921 gctgcattgc agttacgaag aattttgtga aacctggccg cagctggtcg caggtgggct
    37981 ataccccaaa tttggtcact attgcctgat gccactggcg gcggaagggc atatttttgg
    38041 tggctgtgaa tttattcgtt atgacgatcg tccctggagc gaaaaagagt tcaatcgtct
    38101 gcaaacattt acgcagatcg tttctgtcgt caccgaacaa atccagagcc gcgtcgtcaa
    38161 caatgtcgac tatgagttgt tatgccggga gcgcgataac ttccgcatcc tggttgccat
    38221 taccaacgcg gtgctttccc gcctggatat ggacgaactg gtcagcgaag tcgccaaaga
    38281 aatccattac tatttcgaca ttgacgatat cagtatcgtc ttacgcagcc accgtaaaaa
    38341 caaactcaac atctactcca ctcactatct tgataaacaa catcccgccc acgaacagag
    38401 cgaagtcgat gaagccggaa ccctcaccga acgcgtgttc aaaagtaaag agatgctgct
    38461 gatcaatctc cacgagcggg acgatttagc cccctatgaa cgcatgttgt tcgacaccta
    38521 gggcaaccag attcaaacct tgtgcctgtt accgctgatg tctggcgaca ccatgctggg
    38581 cgtgctgaaa ctggcgcaat gcgaagagaa agtgtttacc actaccaatc tgaatttgct
    38641 gcgccagatt gccgaacgtg tggcaatcgc tgtcgataac gccctcgcct atcaggaaat
    38701 ccatcgtctg aaagaacggc tggttgatga aaacctcgcc ctgaccgagc agctcaacaa
    38761 tgttgatagt gaatttggcg agattattgg ccgcagcgaa gccatgtaca gcgtgcttaa
    38821 acaagttgaa atggtggcgc aaagtgacag taccgtgctg atcctcggtg aaactggcac
    38881 gggtaaagag ctgattgccc gcgcgatcca taatctcagt gggcgtaata atcgccgcat
    38941 ggtcaaaatg aactgcgcgg cgatgcctgc cggattgctg gaaagcgatc tgtttggtca
    39001 tgagcgtggg gcttttaccg gtgccagcgc ccagcgcatt ggtcgttttg aactggcgga
    39061 taaaagctct ctgttcctcg acgaagtggg cgatatgcca ctggagttac agccgaagtt
    39121 gctgcgtgta ttgcaggaac aggagtttga acgtctcggc agcaacaaaa tcattcagac
    39181 ggacgtacgt ttaattgccg cgactaaccg cgatctgaaa aaaatggtcg ccgaccgaga
    39241 gttccgtagc gatctctatt accgcctgaa cgtattcccg attcacctgc cgccactacg
    39301 cgagcgtccg gaagatattc cgctgctggc gaaagccttt accttcaaaa ttgcccgtcg
    39361 tctggggcgc aatatcgaca gcattcctgc cgagacgctg cgcaccttga gcaacatgga
    39421 gtggccgggt aacgtacgcg aactggaaaa cgtcattgag cgcgcggtat tgctaacacg
    39481 cggtaacgtg ctgcagctgt cattgccaga tattgtttta ccggaacctg aaacgccgcc
    39541 tgccgcaacg gttgtcgccc aggagggcga agatgaatat cagttgattg tgcgcgtgct
    39601 gaaagaaacc aacggcgtgg ttgccgggcc taaaggcgct gcgcaacgtc tggggctgaa
    39661 acgcacgacc ctgctgtcac ggatgaagcg gctgggaatt gataaatcgg cattgattta
    39721 actgcaaatt gccggacaga tctgcctgtc cggcatacta ttcacgaggt tttttcggac
    39781 gatatttttc cggcagttct ggtaccggac gcttgtcatc gatgagatga cgcacggtta
    39841 agatcggatg acgccacagc attctcggcc cggcccaacg cataatctgt ttcatctctt
    39901 cacgctttgc aggctggtaa cagtgcaccg gacactgctt acaggctggt ttctcttcgc
    39961 cgaacacaca tttatccagc cgcttttgcg cgtaaacaaa caacgcctcg taatgctccg
    40021 gctccgctga cgcctgcggg catttcgctt gataaagatc gatcattttt ttaatcgtca
    40081 gtttttcacg agagatacgc ttgccggaca tgctgcctcc atactcttaa ggtgtattta
    40141 tattacaact taatttgaaa ggggactatg gctctgaagg aggaggttta ttcaaccgac
    40201 gcaattttgc gtacttgctt cataagcatc acgcaaaagc tgcaaaacag catctttccc
    40261 ggaaccagca tcaagaactc gccgttcgct tcttcccctg aaatgattaa ctccggtatc
    40321 atgtgcgcct tatgtgatta caacgaaaat aaaaaccatc acaccccatt taatatcagg
    40381 gaaccggaca taaccccatg agtgcaatag aaaatttcga cgcccatacg cccatgatgc
    40441 agcagtatct caggctgaaa gcccagcatc ccgagatcct gctgttttac cggatgggtg
    40501 atttttatga actgttttat gacgacgcaa aacgcgcgtc gcaactgctg gatatttcac
    40561 tgaccaaacg cggtgcttcg gcgggagagc cgatcccgat ggcggggatt ccctaccatg
    40621 cggtggaaaa ctatctcgcc aaactggtga atcagggaga gtccgttgcc atctgcgaac
    40681 aaattggcga tccggcgacc agcaaaggtc cggttgagcg caaagttgtg cgtatcgtta
    40741 cgccaggcac catcagcgat gaagccctgt tacaggagcg tcaggacaac ctgctggcgg
    40801 ctatctggca ggacagcaaa ggtttcggct acgcgacgct ggatatcagc tccgggcgtt
    40861 ttcgcctgag cgaaccggcc gaccgcgaaa cgatggcggc agagctgcaa cgcactaatc
    40921 cggcggaact gctgtatgca gaagatttcg ccgagatgtc gctgattgaa ggccgtcgcg
    40981 gcctgcgccg tcgcccgctg tgggagtttg aaatcgacac cgcgcgccag cagttgaatc
    41041 tgcaatttgg cacccgcgat ctggtcggtt ttggcgtcga gaacgcgccg cgcggacttt
    41101 gtgctgccgg ttgtctgttg cagtatgcga aagataccca acgcacgacc ctgccgcata
    41161 ttcgttctat caccatggaa cgtgagcagg acagcatcat tatggatgcc gcgacacgtc
    41221 gtaacctgga aattactcag aacctggcgg gcggtgcgga aaatacgctg gcttctgtgc
    41281 tcgactgcac cgtcacgccg atgggtagtc gtatgctgaa acgctggctg catatgccag
    41341 tgcgcgatac ccgcgtgttg cttgagcgcc agcaaactat tggcgcattg caggatttca
    41401 ccgccgagtt gcagccggta ctgcgtcagg tcggcgacct ggaacgtatt ctggcacgtc
    41461 tggctttacg aactgctcgc ccacgcgatc tggcccgtat gcgccacgct ttccagcaac
    41521 tgccggagct gcgtgcgcag ttagaaactg tcgatagtgc accggtacag gcgctacgtg
    41581 agaagatggg cgagtttgcc gagctgcgcg atctgctgga gcgagcaatc atcgacacac
    41641 cgccggtgct ggtacgcgac ggtggtgtta tcgcatcggg ctataacgaa gagctggatg
    41701 agtggcgcgc gctggctgac ggcgcgaccg attatctgga gcgtctggaa gtccgcgagc
    41761 gtgaacgtac cggcctggac acgctgaaag ttggctttaa tgcggtgcac ggctactaca
    41821 ttcaaatcag ccgtgggcaa agccatctgg cacccatcaa ctacatgcgt cgccagacgc
    41881 tgaaaaacgc cgagcgctac atcattccag agctaaaaga gtacgaagat aaagttctca
    41941 cctcaaaagg caaagcactg gcactggaaa aacagcttta tgaagagttg ttcgacctgc
    42001 tgttgccgca tctggaagcg ttgcaacaga gcgcgagcgc gctggcggaa ctcgacgtgc
    42061 tggtgaacct ggcggaacgg gcctataccc tgaactacac ctgcccgacc ttcattgata
    42121 aaccgggcat tcgcattacc gaaggtcgcc atccggtagt tgaacaagta ctgaatgagc
    42181 catttatcgc taacccgctg aatctgtcgc cgcagcgccg tatgttgatc atcaccggtc
    42241 cgaacatggg cggtaaaagt acctatatgc gccagaccgc actgattgcg ctgatggcct
    42301 acatcggcag ctatgtaccg gcacaaaaag tcgagattgg acctatcgat cgcatcttta
    42361 cccgcgtagg cgcggcagat gacctggctt ccgggcgctc aacctttatg gtggagatga
    42421 ctgaaaccgc caatatttta cataacgcca ccgaatacag tctggtgtta atggatgaga
    42481 tcgggcgtgg aacgtccacc tacgatggtc tgtcgctggc gtgggcgtgc gcagaaaatc
    42541 tggcgaataa gattaaggca ttgacgctgt ttgccaccca ctatttcgag ctgacccagc
    42601 taccggagaa aatggaaggc gtcgccaacg tacatctcga tgcgctggag cacggcgaca
    42661 ctatcgcctt tatgcacagc gtgcaggacg gcgcggcgag caaaagctac ggtctggcgg
    42721 ttgctgctct ggcgggcgta ccgaaagagg ttattaagcg cgcacggcaa aaactgcgtg
    42781 agctggaaag catttcgccg aacgccgccg cgacgcaagt ggatggtacg cagatgtctt
    42841 tgctgtcagt gccagaagaa acttcgcctg cggtcgaagc actggaaaat ctcgacccgg
    42901 attcactgac tccgcgtcag gcgctggagt ggatttatcg cttgaagagt ctggtgtaat
    42961 aacaattccc gatagtcttt tgctatcggg aatattaacg acaactgacg aatcaaataa
    43021 aaataccctg tataatagga aagcttattt tacagggtaa aaccatgcca tctacacgct
    43081 atcaaaaaat caatgcccat cactatcgcc gtatatgggt cgttggtgat attcatggtg
    43141 aatatcagtt attacaatcc cgttaacatc aactctcttt ttaccctgaa accgacttac
    43201 ttatttccac aggcgataat attgatcgtg ggccgaagag tcttaacgtg ctgcgcctgc
    43261 taaaccaacc ctggtttacg tcggttaaag gcaaccacga agcgatggcg ttggatgcat
    43321 tcgaaactgg cgatggaaat atgtggcttg ccagcggtgg tgactggttt ttcgatttaa
    43381 atgattcaga gcaacaagag gcaatagatc tgttgctgaa attccatcac cttccacata
    43441 ttattgaaat cattaatgac aacataaaat atgccat
//
LOCUS       DAJVRF010000015        42134 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJVRF010000015 DAJVRF010000000
VERSION     DAJVRF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42134
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.015"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            448..600
                     /gene="mokC"
                     /locus_tag="OQN13_000774"
     CDS             448..600
                     /gene="mokC"
                     /locus_tag="OQN13_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCS1614220.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(704..1834)
                     /gene="dnaJ"
                     /locus_tag="OQN13_000775"
     CDS             complement(704..1834)
                     /gene="dnaJ"
                     /locus_tag="OQN13_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCS1614221.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1923..3839)
                     /gene="dnaK"
                     /locus_tag="OQN13_000776"
     CDS             complement(1923..3839)
                     /gene="dnaK"
                     /locus_tag="OQN13_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCS1614222.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4216..4620
                     /locus_tag="OQN13_000777"
     CDS             4216..4620
                     /locus_tag="OQN13_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCS1614223.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4646..5359
                     /gene="msyB"
                     /locus_tag="OQN13_000778"
     CDS             4646..5359
                     /gene="msyB"
                     /locus_tag="OQN13_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCS1614224.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            5508..6074
                     /gene="satP"
                     /locus_tag="OQN13_000779"
     CDS             5508..6074
                     /gene="satP"
                     /locus_tag="OQN13_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCS1614225.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6109..6696)
                     /gene="mog"
                     /locus_tag="OQN13_000780"
     CDS             complement(6109..6696)
                     /gene="mog"
                     /locus_tag="OQN13_000780"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCS1614226.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6811..7764)
                     /gene="tal"
                     /locus_tag="OQN13_000781"
     CDS             complement(6811..7764)
                     /gene="tal"
                     /locus_tag="OQN13_000781"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCS1614227.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            8043..9472
                     /locus_tag="OQN13_000782"
                     /pseudo
     CDS             8043..9472
                     /locus_tag="OQN13_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            9542..10318
                     /gene="yaaA"
                     /locus_tag="OQN13_000783"
     CDS             9542..10318
                     /gene="yaaA"
                     /locus_tag="OQN13_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCS1614228.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10471..10767)
                     /locus_tag="OQN13_000784"
     CDS             complement(10471..10767)
                     /locus_tag="OQN13_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS1614229.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10981..12267)
                     /gene="thrC"
                     /locus_tag="OQN13_000785"
     CDS             complement(10981..12267)
                     /gene="thrC"
                     /locus_tag="OQN13_000785"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCS1614230.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12268..13200)
                     /gene="thrB"
                     /locus_tag="OQN13_000786"
     CDS             complement(12268..13200)
                     /gene="thrB"
                     /locus_tag="OQN13_000786"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCS1614231.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13202..15664)
                     /gene="thrA"
                     /locus_tag="OQN13_000787"
     CDS             complement(13202..15664)
                     /gene="thrA"
                     /locus_tag="OQN13_000787"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCS1614232.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15691..15810)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15746..15811)
                     /gene="thrL"
                     /locus_tag="OQN13_000788"
     CDS             complement(15746..15811)
                     /gene="thrL"
                     /locus_tag="OQN13_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCS1614233.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(16025..16711)
                     /locus_tag="OQN13_000789"
     CDS             complement(16025..16711)
                     /locus_tag="OQN13_000789"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS1614234.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(17111..17251)
                     /locus_tag="OQN13_000790"
     CDS             complement(17111..17251)
                     /locus_tag="OQN13_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614235.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17347..18063
                     /gene="arcA"
                     /locus_tag="OQN13_000791"
     CDS             17347..18063
                     /gene="arcA"
                     /locus_tag="OQN13_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCS1614236.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(18123..19475)
                     /gene="creD"
                     /locus_tag="OQN13_000792"
     CDS             complement(18123..19475)
                     /gene="creD"
                     /locus_tag="OQN13_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCS1614237.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19533..20957)
                     /gene="creC"
                     /locus_tag="OQN13_000793"
     CDS             complement(19533..20957)
                     /gene="creC"
                     /locus_tag="OQN13_000793"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCS1614238.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20957..21646)
                     /gene="creB"
                     /locus_tag="OQN13_000794"
     CDS             complement(20957..21646)
                     /gene="creB"
                     /locus_tag="OQN13_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCS1614239.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21659..22132)
                     /gene="creA"
                     /locus_tag="OQN13_000795"
     CDS             complement(21659..22132)
                     /gene="creA"
                     /locus_tag="OQN13_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCS1614240.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22343..23212
                     /gene="robA"
                     /locus_tag="OQN13_000796"
     CDS             22343..23212
                     /gene="robA"
                     /locus_tag="OQN13_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCS1614241.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(23209..23856)
                     /gene="gpmB"
                     /locus_tag="OQN13_000797"
     CDS             complement(23209..23856)
                     /gene="gpmB"
                     /locus_tag="OQN13_000797"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCS1614242.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23908..24429
                     /gene="yjjX"
                     /locus_tag="OQN13_000798"
     CDS             23908..24429
                     /gene="yjjX"
                     /locus_tag="OQN13_000798"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCS1614243.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(24514..24840)
                     /gene="trpR"
                     /locus_tag="OQN13_000799"
     CDS             complement(24514..24840)
                     /gene="trpR"
                     /locus_tag="OQN13_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCS1614244.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24930..26867)
                     /gene="sltY"
                     /locus_tag="OQN13_000800"
     CDS             complement(24930..26867)
                     /gene="sltY"
                     /locus_tag="OQN13_000800"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCS1614245.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            27078..28745
                     /gene="ettA"
                     /locus_tag="OQN13_000801"
     CDS             27078..28745
                     /gene="ettA"
                     /locus_tag="OQN13_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCS1614246.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(29052..30284)
                     /gene="nadR"
                     /locus_tag="OQN13_000802"
     CDS             complement(29052..30284)
                     /gene="nadR"
                     /locus_tag="OQN13_000802"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCS1614247.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30305..31687)
                     /gene="radA"
                     /locus_tag="OQN13_000803"
     CDS             complement(30305..31687)
                     /gene="radA"
                     /locus_tag="OQN13_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCS1614248.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31736..32704)
                     /gene="serB"
                     /locus_tag="OQN13_000804"
     CDS             complement(31736..32704)
                     /gene="serB"
                     /locus_tag="OQN13_000804"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCS1614249.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32810..33454
                     /locus_tag="OQN13_000805"
     CDS             32810..33454
                     /locus_tag="OQN13_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCS1614250.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33482..34498
                     /gene="lplA"
                     /locus_tag="OQN13_000806"
     CDS             33482..34498
                     /gene="lplA"
                     /locus_tag="OQN13_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCS1614251.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34530..34679
                     /locus_tag="OQN13_000807"
     CDS             34530..34679
                     /locus_tag="OQN13_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614252.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(34954..35673)
                     /gene="deoD"
                     /locus_tag="OQN13_000808"
     CDS             complement(34954..35673)
                     /gene="deoD"
                     /locus_tag="OQN13_000808"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCS1614253.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35753..36976)
                     /gene="deoB"
                     /locus_tag="OQN13_000809"
     CDS             complement(35753..36976)
                     /gene="deoB"
                     /locus_tag="OQN13_000809"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCS1614254.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(37028..38350)
                     /gene="deoA"
                     /locus_tag="OQN13_000810"
     CDS             complement(37028..38350)
                     /gene="deoA"
                     /locus_tag="OQN13_000810"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCS1614255.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(38477..39256)
                     /gene="deoC"
                     /locus_tag="OQN13_000811"
     CDS             complement(38477..39256)
                     /gene="deoC"
                     /locus_tag="OQN13_000811"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCS1614256.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            39514..41064
                     /locus_tag="OQN13_000812"
     CDS             39514..41064
                     /locus_tag="OQN13_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCS1614257.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGSSSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            41036..41899
                     /locus_tag="OQN13_000813"
     CDS             41036..41899
                     /locus_tag="OQN13_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCS1614258.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
BASE COUNT         9951 a        11408 c        10586 g        10189 t
ORIGIN      
        1 acggaaagtc atatcagcgc taacgggtga ccctaagccc tgataatcat agcttaaggt
       61 caggatttct gagggattaa ttaggaaaag taataattaa tttcttgtca gtcggcgtta
      121 tttgttagga caatatttca aaaataatcc agcttgtcgt caataattgc gataaataga
      181 taaattaaag ttaaataatt aaatcattaa aaaattacgc cacaaaaata ggcgaggcgt
      241 cttcaggatt agaatcgata aaacagaatg ggttaaagag agcgatataa tagcggcggg
      301 tgcttgaggc tatctgtctc aggcattagc tgaacggcag atagagaaaa gccccgagtg
      361 atattttact atcaacccga ggccccctat atgctgaaca catgtagagt gcctcttact
      421 gaccgtaagg tcaaggagaa gagagcaatg aagcagcata aggcgatgat tgtcgccctg
      481 atcgtcatct gtatcaccgc cgtagtggcg gcgctggtaa cgagaaaaga cctctgtgag
      541 gttcacatcc gaactggcca gacggaggtt gctgttttca cggcttacga atccgagtaa
      601 gagcaacggc ggggagtgat ccccgccact tttcaggtgc tcgcatatct tcaacgcacc
      661 ctaattttac ccaagcctgc ccacgggcag gcttttgggg aggttagcgg gtcaggtcgt
      721 caaaaaactt cttcacacca tcaaagaagc tctttgagcg cgggctgttg tgctcaccgg
      781 ttgggccacc gaagctttct tgcagctctt gcagcaactg tttctgcttc tcgttcaggc
      841 ctaccggtgt ttcgacgaca acgcggcaca gcaaatcacc ctgtgcgcca ccgcggacag
      901 acttgacgcc tttaccgcgc atacggaaaa gcttaccggt ctgggtttcg ccaggcactt
      961 tcagtttgac gcgaccatca agggtcggta cttcgatttc gccacccagc gccgccatag
     1021 cgaagttgat cgggacttcg caatagaggt tgttgccttc acgctcgaaa atcgggtgct
     1081 gtttaacctg aacctgaacg tacagatcgc ctgccggtgc gccatgctcg cccgcttcac
     1141 cttcgcccgc aagacggatg cggtctccag tgtccacccc tgccgggatt ttaacggaca
     1201 gcgttttgct gcgctcaaca cgaccatgac catgacattt gttgcacgga tctttgatca
     1261 gcgtaccgcg gccctgacag tgtggacagg tctgctgtac agcgaaaaat ccctggcgca
     1321 tctgcacctg accagaacca tgacaggtcg gacaggtctg cggctgtgta cctggttttg
     1381 caccgctacc gtggcaaacg tcacactctt ccagagtcgg aatgcggatc tctttggtca
     1441 cgccacgtac agcttcttcg agggtgagct ccatgttata gcgtaaatca gcaccgcgcg
     1501 ccgcacgttg acgaccacgt ccgccgccaa aaatatcgcc gaaaacgtca ccaaaaatat
     1561 cgctgaagtc tgcgccgccg ccaaaaccgc cgccgcccat gccaccttgc tcaaacgcag
     1621 catgaccata ctgatcgtat gccgcacgtt tttgcgagtc ggtcagaact tcataagctt
     1681 ccttgatctc tttaaatttc gcctcggcct ctttgtcacc ctggttacgg tccgggtggt
     1741 atttcatggc caggcgtttg taggcctttt tgatttcacg ctcttccgct gttttggaaa
     1801 cgcctaaaat ctcgtaataa tcttgcttag ccatcttttt taaattgccc ctagatgaat
     1861 gcacgggcgg agaggaaatt ccccttcgcc cgtgtcagta taattacccg tttatagggc
     1921 gattattttt tgtctttgac ttcttcaaat tcagcgtcga caacatcgtc atctttcgcg
     1981 ttgtttgcag aagcatcagc accggcagtc tgctgctggg catgttgctg ctgggcgatt
     2041 tccatcagtt tctgggaaac ctgtgccagt tcctgcattt tcgcttcgat agcggctttg
     2101 tcttcacctt tcagagcagt ttccagtgca gtcagcgcag actcgatagc agttttgtcg
     2161 tcagccggca gtttgtcgcc tgcttcttca acctgcttac gggtgctgtg cagcagatgg
     2221 tcgccctggt tgcgagtctg taccagctct tcaaacttac ggtcagcttc ggcgttagct
     2281 tctgcgtcgc gtaccatttt ctggatttca tcttcgttca gaccagaaga agccttgatg
     2341 gtgatcttct gctctttacc gctgttttta tctttcgcgg aaacgtgcaa gataccgtca
     2401 gcatcgatat cgaaggtaac ttcgatctgc ggcatgccgc gcggtgccgg gttgatacca
     2461 tccaggttga actgacccag agatttgtta tcagctgcac gtttacgttc accctgcagc
     2521 acatggatgg ttaccgcaga ctggttgtct tcagcggtag agaacacctg gctgtgcttg
     2581 gtcgggatag tggtattttt cgcgatcagc gtcgtcatca caccgcccat ggtttcgata
     2641 cccagagaca gcggggtaac gtccagcagc agtacgtctt ttacgtcacc agtcagaaca
     2701 ccaccctgaa cagcagcacc gattgctaca gcttcgtccg ggttaacgtc tttacgcggc
     2761 tctttaccaa agaactcagc aactttcttc tgaaccattg gcatacgagt ctgaccaccc
     2821 acgaggataa cgtcgtcgat atcagatacg gacaggccag cgtcctgcag tgcaactttc
     2881 agcggctcaa tggaacggtt taccagatct tcaaccaggc tttccagttt cgcacgagtc
     2941 actttgatgt tcatgtgttt cggaccggtc gcatctgcag tgatgtacgg caggttaacg
     3001 tcggtctgct gagcggaaga cagttcgatt ttcgcttttt ctgccgcttc tttcaggcgc
     3061 tgcattgcca gcggatcgtt gcgcaggtca atgccctgat ctttcttgaa ttcttcaacc
     3121 aggtagttga tcagacggct gtcgaagtct tcacccccca ggtgggtatc accgttggta
     3181 gccagaactt cgaaggtttt ttcgccgtca acttcgtcga tttcgataat agaaatatcg
     3241 aaagtaccac cacccaggtc ataaaccgcg atagtacggt tgccagtacc tttgtccaga
     3301 ccgtaagcca gcgcagctgc ggtcggttcg ttgatgatac gttttacttc cagaccagcg
     3361 atacggcctg cgtctttggt tgcctgacgc tgagcatcgt taaagtatgc cggtacggtg
     3421 ataacagctt cagttaccgg ttcacccaag taatcttcag cggttttctt catttttttc
     3481 agcacttcag cagaaatctg cggcggtgcc attttctggc ctttaacttc gacccatgcg
     3541 tcgccgttat cagcagcaat aattttgaac ggcatgatgg aaacatcacg ctgtacttct
     3601 tcgtcctgga agcggcggcc aatcaggcgt ttaatcgcaa acagggtgtt ttgcgggttc
     3661 gtcactgcct gacgtttagc cggctgacca accagagttt caccatcctg ggtataggca
     3721 ataatagaag gcgtggtgcg atcgccttcg gcgttctcca gcacgcgagg agtggtgcca
     3781 tccataatcg ctacacaaga gttggtagta cccaggtcga taccaattat tttacccatc
     3841 taaacgtctc cactatatat tcggtcatca tgtggttgtg agtctgtaat aggggcgaaa
     3901 cgtctggttt caactgccca atttcatttt tttgcagact cactcactgc ggttgactac
     3961 taaatggggt cgtaacccac gtcatcaagg gggagatgca aaaatttttt gtgcttttgc
     4021 gcagaaattt cgcgatatgg caggacaaga cgtaaaattg acagcaattt tattcatata
     4081 aagaatgaat cgcagccaag gcattcatca aaaaattgta ataaaaagaa aagattacgt
     4141 acctgaatct tctctttatc agcagtaaac tagtgggtat tcatccctct acctcttccc
     4201 actaagagaa tccttatgaa atccgttttt acgatttccg ccagcctggc gattagcctg
     4261 atgctgtgct gcacggcgca ggcaaacgac cataaactcc tcggcgtcat tgcaatgccg
     4321 cgtaacgaaa ccaacgatct ggcgctgaaa cttcctgttt gtcgcattgt gaaacgcata
     4381 caactctctg ccgaccatgg cgatttacag ttaagcggtg catcggttta tttcaaagcc
     4441 gcccgtagcg ccagtcagag tctgaatatt ccttcagaaa taaaagaagg gcaaaccact
     4501 gactggatca acattaacag tgataacgac aataaacgct gcgtctcaaa aatcaccttt
     4561 tccggtcata cggtgaactc atcggatatg gccacgctga aaattatcgg cgacgactaa
     4621 cgccagaata tagaaaccac aaaaaatgaa tgttaattac ctgaatgatt cagatctgga
     4681 ttttcttcag cattgtagtg aggaacagtt ggcaaatttc gcccgattgc tcacccataa
     4741 tgaaaaaggc aaaactcgcc tctccagcgt actgatgcgc aacgaactgt ttaaatcgat
     4801 ggaagggcat cccgagcaac atcgccgcaa ctggcagctg attgctggag aattacagca
     4861 ttttggtggc gatagtatcg ccaacaaact gcgcggacac ggtaaattgt atcgggccat
     4921 tttgctcgat gtttcaaaac gattgaagct gaaagccgac aaagagatgt ctacgtttga
     4981 gattgagcaa cagttactgg aacaatttct gcgtaacacc tggaagaata tggacgagga
     5041 acataagcag gagtttctgc acgcggtcga tgccagggtg aatgagctgg aagagctgct
     5101 gccgctgctg atgaaagaca aattattggc aaaaggcgtg tcgcatttgc tttccagcca
     5161 attgacccgc attttacgca cccacgcagc aatgagcgtg cttgggcatg gtttgctgcg
     5221 cggcgcgggt ctgggaggcc ctgtgggcgc agcattaaat ggggttaaag cggtcagcgg
     5281 cagtgcctat cgcgtgacaa ttccagccgt aatacaaatc gcctgcctgc gccggatggt
     5341 tagcgccact caggtctgac accgtaaacc ggggaaaatt ttcattttcc cctcaaaaga
     5401 tcgtagacac tgccccactg gctgattatt atgccgcgcc ctgaaaacac tactgttatt
     5461 cagggaaatt atttcaccat tcattcgatg atgatttttg aggaattatg ggcaacacta
     5521 agttggctaa tccggcaccg ctgggcctga tgggcttcgg catgaccacc attctgctta
     5581 acctgcacaa cgtgggttat ttcgctctgg acggtattat tcttgccatg ggcattttct
     5641 acggcggcat cgcgcaaatt tttgccggtc tgctggagta caaaaaaggc aacactttcg
     5701 gtttaaccgc attcacctct tacggttctt tctggttgac gctggttgcg attctgctga
     5761 tgccgaaact gggtctgacc gatgcgccaa atgcacagtt ccttggtgtc tacctgggtc
     5821 tgtggggcgt atttacgctg tttatgttct tcggcacgct gaaaggcgca cgcgttctgc
     5881 aattcgtttt ctttagcctg actgtgctgt ttgccctgct ggcgatcggt aacattgccg
     5941 gtaacgccgc aatcatccac tttgccggct ggattggtct gatctgtggt gccagtgcaa
     6001 tctatctggc gatgggtgaa gtactgaacg agcagtttgg tcgcaccgtt ctgccgattg
     6061 gtgaatccca ctaattgttt tgagatcccc ccgctcgggg ggattttttt attcgctaac
     6121 gtcgcgtctt gcactcttcg gtctgaatgc agcaaccact tctggtgccg tttcaacgta
     6181 tggcccttcc agcaactgaa tgcagtacgg tacgctggca aaaataccgt gtaccacaac
     6241 gttacccgca gcgtccttca caccttccag cgtctcttta atagacttcg gctgaccggg
     6301 taagttaagg atcagcgcct gtttgcgaat cacaccaacc tgacgcgaaa ggatcgcagt
     6361 tggtacaaaa tgcaggctta tctggcgcat ctgttcacca aagccaggca tctcgcggtc
     6421 cgctactgcc agcgtcgcat cgggcgttac gtcacgacgc gccgggccag ttccgcccgt
     6481 ggtgagcacc agatggcaac tcatttcatc cactagctca cacaacgttt gctcgatgat
     6541 cgcctgctca tcggggatta agcgggtttc cagttcaaac ggcgtggtta gcgccgatgt
     6601 cagccattct tccagcgcag ggatgccttt atcctgataa acgccgctgg atgcgcgatc
     6661 agagatggaa actaagccaa tacgtaaagt attcatcaag atttttccgt ttaaacagtc
     6721 tcgttaaaca gaatgataca ctgcgaaggg agtgacagac aggcttcaga agaggtagcg
     6781 tgaccgactt cccggtcacg ctaagaatga ttacagcaga tcgccgatca ttttttccag
     6841 tttttcctgg tcaatagcaa acttacggat accttccgcc agtttatcta ctgccattgg
     6901 atcctggttg tgctgccaca ggaactcgga ctcagtgata cgcgccggac gcgctttcac
     6961 ttcgccggtg taagacagtt tacgttcgat agccccttcg ctctcagcca gctctttcag
     7021 cagtgccggt gcgatggtaa gacggtcgca gcctgccagt tccagaattt cgccgatgtt
     7081 acggaagctt gcgcccataa ccacggtttc ataaccgtgc tctttgtagt actggtagat
     7141 ttcagataca gaaaccacgc ccggatcttc tgccggagcg tactctttct tatcagtatt
     7201 cgctttgtac cagtcaagaa tacggccaac aaatggcgag atcaggaaca cgcctgcttc
     7261 cgcacaagca cgagcctgag cgaaggagaa cagcagggtc aggttacagt tgatgccttc
     7321 tttttccagc tgttctgcag cacggatacc ctgccaagta gaagccagtt tgatcagaat
     7381 acgatcgttg ctaataccag catcgttgta gagtttgatc aggcgttttg ctttcgcaat
     7441 tgacgcttcg gtgtcatagg aaagacgcgc atcaacttca gttgagatac ggcccggaac
     7501 cagtttcagg atttccagac caatatttac tgccagtttg tcggtcgcgt ccacgatctg
     7561 ctgcgcgcga tcgttgctct gctgtttcgc ccaggcgaca gcatcatcaa tcaacttacg
     7621 gtattccgga atctgcgctg cgttaagaat gagagaaggg ttggttgtgg catcctgcgg
     7681 ttgatacagc ttcattgccg cgatgtcccc agtgtcggcc actacggtgg tgtactgacg
     7741 aagggaggtc aatttgtccg tcatgatagt atttctcttt aaacagcttg ttagggggat
     7801 gtaaccggtc tgccctgatg atatcacgac gcaacgtcag cgcaaccgcc gacaagacgg
     7861 ttatcgccag gcgagactgt ttcggatttc tgacactgcg ttgatatcgc ccgccatttt
     7921 tatacaaaac ctcatgtatg ctacgcagaa gttatcaagt acctcgtagc gtatatactt
     7981 cttaaacaat tggtaacgtt tacacaggaa agtcatcgcg accggcaata agagggatat
     8041 gcatgccaga ttttttctcc tttattaaca gcgtcctttg gggatcggta atgatttacc
     8101 tgctcttcgg cgcaggttgt tggttcactt ttcgcaccgg atttgtgcag tttcgctaca
     8161 tccgccagtt tggcaaaagt cttaaaaata gcattcatcc acagccaggc ggtttaacct
     8221 catttcagtc attgtgtacc agtcttgcgg cgcgcgtggg tagcggcaac ctggccggcg
     8281 ttgcgctggc tattaccgcc ggtggacctg gagccgtctt ttggatgtgg gttgccgcgt
     8341 ttatcggcat ggcgacctcg tttgccgaat gttcccttgc acaactttat aaagaacgtg
     8401 acgtcaatgg acagtttcgt ggaggaccgg catggtatat ggcgcgcggg ctggggatgc
     8461 gttggatggg cgttctgttc gccgtctttt tgctcatcgc ctacggcata attttcagcg
     8521 gagttcaggc gaacgccgtt gcccgagccc tgagttttct tttgattttc ccccgctggt
     8581 gacaggcatt attctcgctg tctttgctct gctggcaatc actcgcggtc ttcatggcgt
     8641 cgcccggctc atgcaggggt ttgtcccgtt gatggcgata atctgggtgc tgaccagcat
     8701 ggtgatttgc gtaataaata tcgggcaact tccccacgtc atttggtcta tttttgagag
     8761 tgcttttggc tggcaggaag cggcaggcgg cgcggcggga tataccttaa gccaggcgat
     8821 tactaacggt tttcagcgca gtatgttttc caatgaggcg ggaatgggtt cgacgccaaa
     8881 cgcggcagcg gcagcggcgt cctggcctcc gcatccggca gcgcaaggga ttgtccagat
     8941 gattggcatt tttatcgaca ccctggtcat ctgtacggca agcgccatgc tgatattact
     9001 ggcgggtaac gacacaacct acatgccgct ggaaggtatt cagcttatcc agaaggcgat
     9061 gcgggtgcta atgggttcct ggggtgctga gtttgttacc ctcgtggtta ttctgtttgc
     9121 cttcagctcc atcgttgcca actacattta tgccgaaaac aatctcttct ttttacgcct
     9181 gaacaaccct aaagcgatct ggtgtttgcg gatctgcacc ttcgcaacgg tcatcggcgg
     9241 caccttgcta agtcttccgc tgatgtggca actggcagat atcataatgg cctgcatggc
     9301 tattaccaat ttgaccgcca ttttactgct ctcgcctgtg gttcatacca ttgccagtga
     9361 ttatctacgc cagcgtaaac tcggcgtgcg cccggtgttt gatccgttgc gttatccgga
     9421 tatcggtcgc cagctttctc cggacgcgtg ggatgatgtt tcgcaggagt aatcacaact
     9481 atcgatcaac tcattctctt tttttgctaa agtcggcata aatttcctgc aaggactgga
     9541 tatgctgatt cttatttcac ctgcgaaaac gcttgattac caaagcccgt tgaccaccac
     9601 gcgctatacg ctgccggagc tgttagacaa ttcccagcac ttgatccatg aagcgcggaa
     9661 actgacgcct ccgcagatta gcacgctgat gcgcatcagc gacaaactgg cgggtatcaa
     9721 cgccgctcgc tttcatgact ggcagccaga tttcacgccg gagaatgccc gccaggctat
     9781 tttggcgttt aaaggtgatg tctacaccgg cttgcaggcc gaaactttca gcgaagacga
     9841 tttcgatttt gcccaacagc atttgcgaat gctttccggc ttgtatggcg tactgcgccc
     9901 gctcgattta atgcagcctt atcgtctgga aatggggatc cgtcttgaga atgcccgagg
     9961 gaaagatctg tatcaattct ggggagatat catcaccaac aagctgaacg aggcgctcgc
    10021 agcacaaggc gataatgtgg tgattaatct ggcgtcagat gagtatttta aatcggtgaa
    10081 gccgaagaaa ttgaatgccg agattatcaa gccggtgttc ctcgatgaga agaacggcaa
    10141 atttaagata atcagcttct acgctaagaa agcacgcggt ctgatgagtc gtttcattat
    10201 tgaaaatcgg ctgaccaaac cagagcaact gactggtttt aatagcgaag gttacttctt
    10261 tgatgaagct tcctccagca atggcgaact ggtgtttaaa cgctacgagc agcgttaagt
    10321 ttgcagtcaa tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag catgcagatt
    10381 caatatattg cagaattcac gtaggcctga taagacgcgc cagcgtcgca tcaggcgctg
    10441 aatgccggat tgttacccgg catttgtcat ttagcggtga tgtttgcctg gaccatgacc
    10501 gccgtgatga tcatgaggag ctttcttatg gtggcgcggc ggtggcggcg gtccgtgtgg
    10561 gtgccagcga ttgcctcgcc attcataatg ttgtttccac cagccgtggt cgcgccagtg
    10621 acctccatcc cagtaatagc cacgattatc acgatcgcct atctgtaatt ttactgacgg
    10681 gactaacgta atttccgcag cctgtgctgc catgggagcg accagaacca gggaaagtgc
    10741 gagtacgata gattgcatct ttttcacagg ttactccttt tatcattgcc cgttatgggt
    10801 cgatatccgg agattacgcg atgagaacga cggttgttat tctccgcaat cctaatctct
    10861 aagttaccgc atttattgag aatttctcct ttttccctgt cgtttttctc cataatttct
    10921 tcataaaaaa gccgggctgc ataaaagcaa acccggcctg attgagataa tgaatagatt
    10981 ttactgatga ttcatcatca atttacgcaa cgcagcaaaa tcggcgggca gattatgtga
    11041 aagcaagggt aaatcagcac gttctgccag ctcttttggc agatccaacg tttcaccgag
    11101 aatcgcttcc acgctctctt taaatttcgc cggatgcgcg gtgccgagga acaagccata
    11161 ttcgcctgga ttcaactggt cacacagcgc acgatacgct acggcagcgt gcggctccga
    11221 ggtgtagccc agttctttta actcacgcat tgtctgttgc gtggtttcat catccacggc
    11281 tgcataaccc agctctttca gttgccagat tttgcggcgg aacaactctt ccacacgcgg
    11341 ccagttgttc ggctgactca cgtccatcgc gttggataac gtcgcctgag tcgctttggg
    11401 tgaccactga ccgtcgtgca ggaaacgtgg cacggtatcg ttcacgttgg tcgcagcaat
    11461 aaaacgtttc accggcagac cgagtgactt cgccagcaga cccgccgtca aatcgccgaa
    11521 gtttccgctt ggcaccgaga caaccagctg gttgcgcgct tcctgcggca gctgcgcaac
    11581 tgcttcaaag tagtagcaaa tctgcgccag caaacggctg atgttaatcg agttagccga
    11641 gtttaacccc agcgccactt tcagttcttc atcatcaaac gcctgcttca ccagcgcctg
    11701 acaggcatcg aaatcgccgt cgatggcaac agtttcgata ttgccgccca acgtacagaa
    11761 cagtttttct tgcagtggac tgattttgcc tcgtggatag aggataacca ctttcacatt
    11821 cggcaaaccg tagaaagcat gagccactgc cgctccggta tcaccggagg tcgcggtcag
    11881 aatggtcact ggcttatcgc ccgcaatatg ggtcagcatt tgtgccataa agcgaccgcc
    11941 gaaatcttta aatgccagcg ttggcccgtg gaacaattcc agacaaccga catcgctttc
    12001 aacattggcg accggagccg ggaaggcaaa cgccgcgcgc acgcgctctt ccaggatttc
    12061 ctgcgggatt tcatcaccaa taaacgccga gaggatcttc gcactgcggg tgacaaaatc
    12121 cagcttcagc atctcatcaa tttcagtcag gctgaattcc ggcaggtcat gcggaaaaaa
    12181 cagcccctga tttttgccca acccctgggt tacggcttgc gcaaagctga cctgctcgtt
    12241 gtgatctttc agattgtaga gtttcattta gttttccagt actcgtgcgc ccgccgtatc
    12301 cagccggcaa atatgaacaa aaccttcctg attttgcagg tagttcttac ccaaccagtc
    12361 ggcaacgcgc tgggcggtat ccggcttgtc acatagagcg aacaaggtcg ggccggagcc
    12421 ggagataccg ctcgctaccg cgccgatttc cgcgaccgcc tgccgcgcct gccggaagcc
    12481 aggcagtaac cgttcacggt agggttcagc gataacatct ttcatcagct tcgcggcaag
    12541 ctcaggctga cgggaatagc aggcgtgaat gaagccagcc agatgtcgcc cgtgcgcaat
    12601 gcaatcctgg cggcgatact gcgccggtaa aatagcccgg gcttctgccg tcgagacttt
    12661 aattcccgga tacgccagca cccacagcca ctcatcaaac cctggcactt gctggctgat
    12721 gatgtcgttt tcttcgatca tcaactgcat accaccaaga aaacacggtg ccacgttgtc
    12781 gtaatgaatg ctgccggaga tacgtccttc cagctcgccc atcaaagcca gcaaacgggt
    12841 gtcattaagt ggcttgccgc agtgttcatt catcgccatc agcgccgcga ccaccgaaca
    12901 ggcgctggag cctaagcccg agccgatcgg catattcttt tccagagtca tcgccactgg
    12961 aatttgcttg cccagctcca ggcaaaaacg ctcccagcac tgataaacga tattttcccg
    13021 tggttctgac ggcagcttat cggcaaagcg tccgaggttg ttgagactga atgtctctgc
    13081 cgcctcaacc gtgactacat ctccgagcaa tgcaccatca acgggtgtca ccgccgcccc
    13141 gagcacatca aacccgacgc tcatattggc actggaagcc ggggcataaa ctttaaccat
    13201 gtcagactcc taacttccat gagagggtac gtagcagatc ggcaaagaca ccagcagcgg
    13261 taacgtcatt gcccgcacca tatccgcgca gcaccaacgg cagcggctga taatagtggc
    13321 tataaaaggc cagggcgttt tcgccatttt tcactttgaa cagcggatca ttcccatcca
    13381 cttcggcaat cttcacgcgg cagacaccat cttcatcaat attgccaaca tagcgcaaaa
    13441 cttttccttc atcacgggcc ttcgccacgc gcgcggcaaa gagatcgtcg agctgtgaca
    13501 gattcgccat aaaagcggca acatcaccct cggcgttaaa ctctgcgggc agcacaggtt
    13561 caatttcaat atccgccagt tccagttcac gtcccgtttc acgagcgaga atcaatagtt
    13621 tacgcgccac atccatacca gaaagatcat ctcgcgggtc cggttcggta taacccattt
    13681 cccgcgccag cgtggtcgcc tcggagaaac tcatgccttc gtctaacttg ccgaagatat
    13741 aagaaagcga acctgaaaga atgccggaga acttcaccaa ttcatcacct gcattgagca
    13801 gattttgcag gttctcaata accggtaatc cagccccaac gttggtgtca tagaggaatt
    13861 tacgccgcga tttttccgcc gcataacgca actgatgata gtaatccatc gacgaggtgt
    13921 tggccttttt gttcggcgtg acaacgtgga aaccttcgcg caggaagtcg gcatattgat
    13981 ccgccactgc ctggctggag gtgcagtcaa caatgaccgg gttcagcaga tgatattctt
    14041 tcacgaggcg aattaagcgc ccgagattaa acggctcttt ggcttgtgca agttcttcct
    14101 gccagttttc cagattaagg ccatgcacac tagtgagcag agccttcgag ttggcaacgc
    14161 cgcagacacg taagtcgata tgtttattct tcagccagct ttgctgacgc ttcagttgct
    14221 ccagcagcgc accgccaacg ccaccgacgc caatcacaaa cacttcgata acctgatcgg
    14281 tattgaacag catctgatga gtaacgcgca cgccagtggt cgcatcatcg ttatttacca
    14341 cgacagagat tgagcgttca gaagatccct gagcaatggc gacaatgttg atattggcgc
    14401 gggccagcgc ggcaaagaat ttcgccgaga tcccacgcaa ggtgcgcata ccatcaccta
    14461 ccaccgagat aatggccagc cgttccatca ccgccagcgg ctccagtaag ccttctttca
    14521 gttccaggta gaactcttcc tgcattgccc gttcagcccg cacacagtcg ctttgcggaa
    14581 cgcagaaact gatactgtat tcggaagatg attgcgtaat cagcaccacg gaaatacggg
    14641 cgcgtgacat cgcggcaaag acgcgcgccg ccatgccgac cattcctttc atccccgggc
    14701 cggaaacgct gaacattgcc atgttattca gattggaaat gcccttgacc ggtaattcgc
    14761 cttcatcacg actggcacca atgagcgtac ctggtgcttg aggatttccg gtatttttaa
    14821 tcaggcaagg gatctggaac tgggcgatgg gggtaatggt gcgggggtga agaactttag
    14881 cgccgaagta ggaaagctcc atcgcttcct ggtaggacat cgacttcaac aacctcgcat
    14941 cgggcacctg acgcgggtcg caggtataga ccccgtcaac gtccgtccaa atctcgcaac
    15001 aatcggcgcg taaacaggca gccagcaccg ccgcggagta gtcggaaccg ttgcgtccaa
    15061 gtaccaccag ttcgcctttt tcattaccgg cggtgaagcc tgccatcagc accatgtgat
    15121 cagccggaat acgacttgcc gcaatacggc gggtggactc agcaatatcg acggtagatt
    15181 cgaggtaatg ccccactgcc agtagttttt cgaccggatc gataacggta acgttgtgac
    15241 cacgcgcttc taacacgccg gccataatgg cgatcgacat tttctcgcca cggcaaatca
    15301 gcgcagcgtt aatgctatcc gggcactgcc ctaacaaact aatgccatgc aggacatgtt
    15361 ttatttgggc aaattcctgg tcgacgaaag ttttcaattg cgccagcggg aatcccggct
    15421 gggcggcggc gagtcccgtc aaaagttcgg caaaaatacg ttcggcatcg ctgatattgg
    15481 gtaaagcatc ctggccgcta atggtttttt caatcatcgc caccaggtgg ttggtgattt
    15541 tggcgggggc agagaggacg gtggccacct gcccctgcct ggcattgctt tccagaatat
    15601 cggcaacccg cagaaaacgt tctgcatttg ccactgatgt accgccgaac ttcaacactc
    15661 gcatggttgt tacctcgtta cctttggtcg aaaaaaaaag cccgcactgt caggtgcggg
    15721 cttttttctg tgtttcctgt acgcgtcagc ccgcaccgtt acctgtggta atggtgatgg
    15781 tggtggtaat ggtggtgcta atgcgtttca tggatgttgt gtactctgta atttttatct
    15841 gtctgtgcgc tatgcctata ttggttaaag tatttagtga cctaagtcaa taaaatttta
    15901 atttactcat ggcaggtaac cagttcagaa gctgctatca gacactcttt ttttaatcca
    15961 cacagagaca tattgcccgt tgcagtcaga atgaaaagct gaaaaatact tactaaggcg
    16021 ttttttattt ggtgatattt ttttcaatat catgcagcaa acggtgcaac attgccgtgt
    16081 ctcgttgctc taaaagcccc aggcgttgct gtaaccagtc gaccagtttt atgtcatctg
    16141 ccactgccag agtcgtcagc aatgccatga ctcgttcgcg taaagcttgc agttgatgtt
    16201 ggtctgccgt tgtgtcactt ttcgccggtt gttgtattaa tgttgctaat tgatagcaat
    16261 agaccatcac cgcctgcccc agattgagcg aaggataatc cgccaccatc ggcacaccag
    16321 taagaacgtc agccaacgct aactcttcgt tagtcagccc ggaatcttcg cgaccaaaca
    16381 ccagcgcggc atggctcatc catgaagatt tttcctctaa cagcggcacc agttctactg
    16441 gcgtggcgta gtaatgatat ttcgcccgac tgcgcgcagt ggtggcgaca gtgaaatcga
    16501 catcgtgtaa cgattcagcc agtgttggga aaactttaat attatcaata acatcaccag
    16561 atccatgtgc gacccagcgg gtggctggct ccaggtgtgc ctgactatcg acaatccgca
    16621 gttcgctaaa acccatcgtt ttcattgccc gcgccgctgc cccaatattt tctgctctgg
    16681 cgggtgcgac caggataatc gttatacgca tattgccact cttcttgacc aaataaccgc
    16741 gaacagggtg atcactgtca acttattacg cggtgcgaat ttacaaattc ttaacgtaag
    16801 tcgcagaaaa agccctttac ttagcttaaa aaaggctaaa ctatttcctg actgtactaa
    16861 cggtttaatt gttaaaaaat gctacatatc cttctgttta cttaggataa ttttataaaa
    16921 aataaatctc gacaattgga ttcaccacgt ttattagttg tatgatgcaa ctagttggat
    16981 tattaaaata atgtgacgaa agctagcatt tagatacgat gatttcatca aactgttaac
    17041 gtgctacaat tgaacttgat atatgtcaac gaagcgtagt tttattgggt gtccggcccc
    17101 tcttagcctg ttatgttgct gttaaaatgg ttaggatgac agccgttttt gacactgtcg
    17161 ggtcctgagg gaaagtaccc acgaccaagc taatgatgtt gttgacgttg atggaaagtg
    17221 catcaagaac gcaattacgt actttagtca tgttacgccg atcatgttaa tttgcagcat
    17281 gcatcaggca ggtcagggac ttttgtactt cctgtttcga tttagttggc aatttaggta
    17341 gcaaacatgc agaccccgca cattcttatc gttgaagacg agttggtaac acgcaacacg
    17401 ttgaaaagta ttttcgaagc ggaaggctat gatgttttcg aagcgacaga tggcgcggaa
    17461 atgcatcaga tcctctctga atatgacatc aacctggtga tcatggatat caatctgccg
    17521 ggtaagaacg gtcttctgtt agcgcgtgaa ctgcgcgagc aggcgaatgt tgcgttgatg
    17581 ttcctgactg gccgtgacaa cgaagtcgat aaaattctcg gcctcgaaat cggtgcagat
    17641 gactacatca ccaaaccgtt caacccgcgt gaactgacga ttcgtgcacg caacctgctg
    17701 tcccgtacca tgaatctggg tactgtcagc gaagaacgtc gtagcgttga aagctacaag
    17761 ttcaatggtt gggaactgga catcaacagc cgttcgttga tcggccctga tggcgagcag
    17821 tacaagctgc cgcgcagcga gttccgcgcc atgcttcact tctgtgaaaa cccaggcaaa
    17881 attcagtccc gtgctgaact gctgaagaaa atgaccggcc gtgagctgaa accacacgac
    17941 cgtactgtag acgtgacgat ccgccgtatt cgtaaacatt tcgaatctac gccggatacg
    18001 ccggaaatca tcgccaccat ccacggtgaa ggttatcgct tctgtggtga tctggaagat
    18061 taatcggctt taccaccgtc aaaaaaaacg gcgcttttta gcgccgtttt tatttttcaa
    18121 ccttatttcc agatacgtaa ttcatcgtcc gttgtaactt ctttactggc tttcattttc
    18181 ggcagtgaaa acgcatacca gtcgatatta cgagtcacaa acatcatgcc ggccagcgcc
    18241 accaccagca cactggttcc caacaacagc gcgctatcga cagagttgag cagtccccac
    18301 atcactccat ccagcaacaa cagcgcgagg gtaaacaaca tgctattgcg ccagcctttc
    18361 aataccgcct gcaaataaat accgttcatt aacgccccaa tcagactggc gattatccat
    18421 gccacggtaa aaccgatatg ttcagaaagc gccagcaaga gcaaataaaa catcaccaat
    18481 gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt gcgcggtgag cgtttcaaaa
    18541 acaaagaacg ccataaaagt cagtgcaatc agcagaatgg cgtacttagt cgcccggtcc
    18601 gttaattggt attgatcggc tggcgtcgtc actgcgacgc taaacgcgag gaagttttcc
    18661 cagccggtat cattgcctga agcaaaacgc tcaccgagat tattagcaaa ccagctgctt
    18721 tgccattgcg cctgaaaacc tgactcgcta acttcccgtt tggctggcaa aaaatcacct
    18781 aaaaaactgg gatgcggcca gttgctggtt aaggtcattt cgctattacg cccggcaggc
    18841 accacagaaa gatcgccggt accgcttaaa ttcagggcca tattcagctt caggttctgc
    18901 ttccgccagt ccccttcggg taaagggata tgcacgccct gcccgccttg ctctaacccg
    18961 gtgccgggtt caatggtcag cgccgttccg ttaacttcag gcgctttcac cacaccaata
    19021 ccacgcgcat ccccgacgct aatcacaata aatggcttgc caaaggtgat atttggcgcg
    19081 tcgagttcgc taagacgcga aacatcgaaa tcggctttta gcgttaaatc actgtgccag
    19141 acctgaccgg tataaatccc tatcttgcgt tcttccacgt tctgattgcc atcaaccatc
    19201 aatgactcag gtaaccaaaa atggataaaa cttcgtttcc gctccacggt tttatcctct
    19261 tcctgtaccg tataaagctc ggtcacagga atagcgataa gcggcccaac aagtttttgc
    19321 ggcccgctgg tactttggcg aatcgcatct tccacatcgc tacggtaatc ggcacgttcg
    19381 acaatcacct gccgaatcag cattatcgga atcaacaaca gcaatactgc accaaacagg
    19441 gtggtcattt tccagaacag ggaggatttc aacatagtct tctcctttgc aatggcagca
    19501 ggatacgaag gctatgtggg aagaatttga agctatgtga agtgacggtg aagtcgaagc
    19561 gaggccagca cgccaccttc ctgcacgttg cgcagcgtga cttcgccgtt aaacaaacgg
    19621 gcgacctcac tgacgaacgc caaccccaga ccgctgcttt tttgcccatt tgcacgcggc
    19681 aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt caggaatacc actaccggta
    19741 tccagcacct taagcgtgac gtgttcctga tccacttcgg cgcttagcgt tatacgaccg
    19801 ctctcggggg taaaatcgat ggcgttatcc agtaaattcc ccagcgcctg atccagtaac
    19861 gccggttcag cagcaacatt aacctcggtg ggcgtaacat gtagagtgat gtttttttct
    19921 gccaactgca cggtgcgcgc ttcgctgacg cggcggaata atgccgccac atcaacagca
    19981 gtcaaaacga cttcctgacg attctccagt cttgcctggc gtagtaacgt ttccaccagt
    20041 gcctgcattc gcgcattttg cgtcagaatg ttgtcagtaa aacgagccac tacttcaggc
    20101 ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg ccgccagtgg gctttttagc
    20161 tcatgggtta acgcataaac atactgctca atatagtttt tcccttccag cttcacgcgc
    20221 atactttcca gcgcctgcgc gagtttacgc agctcgctac taccgagatc gggaagagga
    20281 acgggcttat tgtcagtgac ggaatcagca tagcgagtga gccgggcaat ggagcggttg
    20341 atccaccaaa ccatgcctgc gccaatcacc agtgcgatcc ccaacaaaat ggcgctggcc
    20401 cataaaattc gccgctcgct gcgtttaatg accggagcca tcgccgcgtt cggtttgcct
    20461 acgctcaaaa cgccaataag ccgcgagccg tccataatcg gcgcggcaac atacatcacc
    20521 gaactttcgg gatcggcagg attttgcaac gtgctacgcg caccatactg accacgcaac
    20581 gttagccaga cgtcattcca gcgcgaataa tcctgcccaa cggctttatt tgccgaatcg
    20641 aacaatactt tgccctgcgc atcggtcata tagacatgat attcattgcg cactttgtta
    20701 atgccaccga tattggcgcg aaacgggcga tgttgtagct gattaaacgc ctgagccagt
    20761 tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg ccagctccgc cagcaacgtt
    20821 gcggtatcga ttaacgtccc ctccgttgct cttcgcacgc ccggtttaac ttctttgaca
    20881 aaaatagcca gtacgaacca ggccgccacc gccaccagta aaaaatagcc cagcagcaac
    20941 cgcatgccga tacgcattac aggcccctca ggctatatcc catgccgcga tgagtattaa
    21001 tcggagaaag atcggggttg atggcgcgca gcttggcacg caacgtttta atgtgggtat
    21061 cgacggtgcg atcgtaggtg tcctgcgcat cttcccacac gctatccatt agttgctggc
    21121 gggaccagac gcggcctggc gacttgagta acgtcttcag caataaaaac tcataccgcg
    21181 tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc gggttcattc aattcaaaat
    21241 gaccaatacg gatgacggga gacggcgacg agaacttctt cacccgacgc agtaaggtgc
    21301 gcaccctggc gcacacttcg cggggtgaaa acggtttagc cacatagtcg tcagcgccaa
    21361 tttccagccc aagcaggcga tcgacctctt cacttcgggc cgtcaggaac agtacaggta
    21421 acgccggatg gagcgccagt aactggcggc acaattcaaa gccgctgata tcaggcaaac
    21481 caacatcgag aatcatgacg tcgggaacct gctggcgagc tttatccagc accggcaagc
    21541 ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa catgtagacc agcgtgtcgg
    21601 ctatcccttg ctcatcttcc actaaccaga ccgtttcccg ttgcataaat cgcctctgtt
    21661 attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt tttcggcgaa ccttctacaa
    21721 ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt ggcgtcataa aagcgcacga
    21781 cctgtaacga cttaaagacc agggacgtgc gttttttgaa tactacctcg ccctgagctt
    21841 tgccgttttt aatacgatcc gacagttcaa tcggcccgac ttgctgacaa gaaatagccg
    21901 catcggaggt atcttccgcc agacccaatc cccctttaat accacctgtt ttcgcccggc
    21961 tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa ggcttccaca acaattttgt
    22021 gatccgggcc gatcatttta aatacggtgt cgaccgaacc aatctcttct gcatgagcca
    22081 atggccccag cattatcagg cttaaagaca ggatcaaatg cttgtatttc atattgttac
    22141 catttttatc tattacacga gcaattagtt cgtcacggta aataacatat tgattaagaa
    22201 caaaatctca atacttttat ttccgtcaag ccctaaaaca tactctacta agaaaaaaac
    22261 actgaatgct aaaacagcaa aaaatgctat tatccaatta cctgatgtca ggtgctcgtt
    22321 gttgaaagga tgaggatatt ttatggatca ggccggcatt attcgcgacc ttttaatctg
    22381 gctggaaggt catctggatc agcccctgtc gctcgacaat gtagcggcga aagcaggtta
    22441 ttccaagtgg cacttacaga gaatgtttaa agatgtcact ggccatgcta ttggcgcgta
    22501 tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta cgcctgactg cgcgtccgat
    22561 tctggacatc gcgctgcaat accgcttcga ctctcaacag acatttaccc gcgcattcaa
    22621 gaagcagttt gcccagactc ctgcacttta ccgccgttct cctgaatgga gcgcctttgg
    22681 tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca gagcacaaat ttgtcaccct
    22741 ggaagatacg ccgctgattg gtgttaccca gagctactcc tgttcgctgg agcaaatctc
    22801 tgatttccgc catgaaatgc gttatcagtt ctggcacgat tttctcggca acgcgccgac
    22861 cattccgcca gtgctctacg gcctgaatga aacgcgtccg agtcaggata aagacgacga
    22921 acaagaggta ttctatacca ccgcgttagc ccaggatcag gcagatggct atgtactgac
    22981 ggggcatccg gtgatgctgc agggcggcga atatgtgatg tttacctatg aaggtctggg
    23041 aaccggcgtg caggagttta tcctgacggt atacggaacg tgcatgccaa tgctcaacct
    23101 gacgcgccgt aaaggtcagg atattgagcg atactacccg gcagaagacg ccaaagcggg
    23161 agatcgccca attaatctgc gctgtgaact gctgattcct atccgccgtt aacgctgcag
    23221 ctcatctaat gcaggggcgt ccagatgcga gatgtcccct gcggtttcaa cgacccagcc
    23281 agacgccagc cacaggcttt cctgataatc cacgcgcgaa atagaacagt tacgcagacg
    23341 taagcggcgc tctgcccatg ctggtaatcc gagaatcgta ctcaccagac atcccagtgc
    23401 aataccatgg ctcaccagca gcggtcggct tccctgcggt aagtcccggc aagattccag
    23461 tgccgcattg acgcgatcgc tgagctcctg cattgactcg ccttcaggaa tacgcccgtc
    23521 aacggtgcca ttgaccagct gccgacgcca gttctcttct tcttcggtca gagaatcgat
    23581 atgtcttttc tccagcacac ccatgtttaa ttcacgcagg cgagaatcaa agatgatgtc
    23641 acagccgcag gcctgggcga tgatttccgc cgtacgccgg gtgcgtccta aatcgctact
    23701 gataatatgc gtaatgccaa gctctttggc acgggttgcc acctgcatcg cctgttgctc
    23761 acctttggcg gtcagcgggc tgtcagactg gccctgaata cgtcgctcgg cgttccactg
    23821 cgtttcaccg tggcggacta ggtatacctg taacatgctt tttttccgta tactgctgta
    23881 acgttaattt tctgagtaat gctgattatg caccaagtta tctgtgcgac caccaatccc
    23941 gctaaaattc aggccattct gcaggcattt cacgagatct tcggcgaagg atcctgccat
    24001 attgcatccg ttgccgtcga gagcggtgta ccggaacagc cctttggcag tgaggaaacg
    24061 cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt tacttccaga ggctgatttt
    24121 tgggtggcga ttgaagctgg catcgatggt gacagcactt tcagttgggt ggtgattgaa
    24181 aacaccagcc agcgcggcga ggcgcgttcg gctactttgc cgttacctgc ggtgattctg
    24241 cagaaagtgc gtgaaggtga ggcgcttggt cccgtgatgt cgcgttatac cggtattgat
    24301 gagattggtc gtaaggaagg ggcgatcggc gtgtttaccg ccgggaaact cactcgcgcc
    24361 agcgtctatc accaggcggt gatcctggcg ctgagtccgt ttcacaatgc cgtgtatcag
    24421 gcattgtagg acggataagg cgttcacgcc gcatccggca ttcggtgcac gatgcctgat
    24481 gcgacgctgc cgcgtcttat caggcctaca aaatcaatcg cttttcagca acacctcttc
    24541 cagccactgg cgcagctcca cgggcgcggc tttcaggctg ttagatccac gcgtaatcgt
    24601 cgcgatgccc gcgccgagtt cattttttaa ctcacgctga ctcatttcgc cacgcaacag
    24661 ctcttcgaca atacgcacgc gagtccccaa cgcttcgcgc tcatctggcg tcagcatcag
    24721 gtttaacaac ggtaaatgga gatcgttttg gtaggcattc ttaagcaggt cgacaaaacg
    24781 taaccactcc tggtgacgct gttctgccat cgctgctgaa tagggtgatt gttgggccat
    24841 aatatgtcgc cattgttagc gggggaagca aaatgcctcc cccggttgta ctcgctaaag
    24901 agtacgatag catatcataa acgtgcggat cagtaacgac gtccccattc cgtggcgctc
    24961 atcaacgtcg gtttatcccc catgaaataa cggtagtaag cgtcataagc cagcacgttc
    25021 ttcacataac cgcgcgtctc ggagaacgga atactctcga caaatgccac agcgtcgata
    25081 cgcccggcgc tgttgccaag ccaggttcgc acccgtccag gtccggcgtt ataagctgct
    25141 gaggagaaaa tacggttatt gccaaactgc tgataaacat attgcaggta actggtgcca
    25201 atgttgatat tcgtttccgg atccagcaat tgcccagggc tgctataacc aggaatagag
    25261 aacatcttca ccgtatgggt cgctgtacca ggcataatct gcatcaggcc gctggcccct
    25321 accggtgatt tcactttcgg attccaggcg ctctcctgac gggcaatcgc catcgcatag
    25381 ctttgcggga tctctttccc actggtgtaa cgtttgaaaa gatcgttgta agccagcggg
    25441 aatcgctctt ccagatgatc ccaaagcttc ccggcgatcg ttgcctgaac gctaagatcc
    25501 caccattggt tattgaaagc ataccgagcc agttgagcct gctctgtttt tgacttgctc
    25561 ttcaccagat tggcccactc gctacgcgcg gtgttatcga gattccagta catcaactcg
    25621 cgcacgcgcg ccatctccgg cccctgagtc agggcgctgt caacattctg cggcgcttta
    25681 tcaatcttca gctcatactc ttctccgatg cgttgtgctg caaccatcgg gtagaaacca
    25741 cgctgttgca tgagttgatg caaaatctct ttcgcttcag cttcacgtcc gcgttccagc
    25801 aataaatcca cctgccagta acgccattca tctttctctt ttgcttccat cggcagacgc
    25861 gccagccagg tattcaggcc gcgacgatcg ccggtgccaa gtgccattcg cacacggcgt
    25921 tcaatcaacg acgtggattg cgagcgcata atgacgtcat cgcgccattt cgcctgctcg
    25981 tcggtgacat cgttgcccat caaacgccag gcgacgatat cgcgcagctc ctgaatctga
    26041 tcttcattaa gctgctgcac ctgggcaagc gacgggatca tcagccgtgc attttcagca
    26101 tcctgccgcg ccacactggc aaacgccacc gctgccattt gacgggtaaa atcggtcgct
    26161 ccggttgtac gcgcgaaggt cagtaccgta ttagggttgt tcgccagtga aatgattgcc
    26221 gaggcgatag tctggtaatc ggcaggcatc tgccctgcca gcactgttac caggccagtg
    26281 ttacccgctt tcatcgccag acggatacgc tctaaatacg ccagcggatc ttgtttacct
    26341 gacgcacgcc agacgctaaa taacttgtca caggcgttag gctggctctt gccggttagc
    26401 cacagctctt tcgccccttg ccaggcttct tcactctgcc cggtgttcca tttcgcatag
    26461 tagtaattac attgcgcttc ggtagttccg ggcttttccg ggctaaaggc taacaagcca
    26521 cgccagtctt cacgccgcgc cagttcattg acgaaacgag attgcagcgt gcgagcggga
    26581 ggaagcgtgg ggttagcgcg aacaaagtta gtgaccgtca ccgccggttg attcatcaga
    26641 tcatcggtga tctggcggta ttccaggtag ggataaagcg gataatcctt cagtccaggc
    26701 atcatttgtt cgaccacatc catttgtcga ttatcccagg cctgcttgat ttgcgcgtaa
    26761 cggctacgct gctcatccag tgagtcggct cgcgccacgc tgctgaccgt cagcagacag
    26821 acaccggcag ccaacagccg ccaggtaact tgtttggctt tttccacaag cgcatcctct
    26881 aagtgtaaat acatcaatgc agcgtcatgc cgcgtaatag atttatgcta accagtcatt
    26941 gccgtttacg ccacgttacg gacacttttt tacttttact gcgaaggtga tcgaccaact
    27001 gggattaggc ggggaaaaag gctacacttc gcctttgaaa actcatcatc acgataaaca
    27061 tagaggcgaa gtccaacgtg gctcaattcg tttataccat gcatcgtgtc ggcaaagttg
    27121 ttccgccgaa acgtcatatt ttgaaaaaca tctctctgag tttcttccct ggggcaaaaa
    27181 ttggtgtcct gggtctgaac ggcgcgggta agtctaccct gctgcgcatt atggcgggca
    27241 ttgataaaga catcgaaggt gaagcgcgtc cgcagccaga catcaagatt ggttacctgc
    27301 cgcaggaacc gcagctgaac ccggaacaca ccgtgcgtga gtccattgaa gaagcggttt
    27361 ctgaagtggt taacgccctg aaacgcctgg atgaagtgta tgcgctgtac gccgatccgg
    27421 atgccgattt tgacaagctg gccgctgaac aaggccgtct ggaagagatc attcaggctc
    27481 acgacggtca taacctgaac gtacagctgg agcgtgcggc ggatgcgcta cgtctgccgg
    27541 actgggacgc gaaaatcgct aacctctccg gtggtgagcg tcgtcgcgta gcgttgtgcc
    27601 gcctgctgct ggaaaaacca gacatgctgc tgctcgacga accgaccaac cacctggatg
    27661 ccgaatccgt ggcctggctg gaacgcttcc tgcacgactt cgagggcacc gtggtggcga
    27721 ttacccacga ccgttacttc ctcgataacg ttgcaggctg gatcctcgaa cttgaccgcg
    27781 gtgaaggtat tccgtgggaa ggcaactact cctcctggct ggagcagaaa gatcagcgcc
    27841 tggcgcagga agcttcacaa gaagcggcgc gtcgtaagtc gatcgagaaa gagctggagt
    27901 gggtacgtca gggaactaaa ggccgccagt cgaaaggtaa agcacgtctg gcacgctttg
    27961 aagagctgaa cagcaccgaa tatcagaaac gtaacgaaac caacgaactg tttattccac
    28021 ctggaccgcg tctgggcgat aaagtgctgg aagtcagcaa cctgcgtaaa tcctacggtg
    28081 atcgcctgct gattgatgac ctgagcttct cgatcccgaa aggggcaatc gtcgggatca
    28141 tcggtccgaa cggcgcgggt aaatcgaccc tgttccgtat gatctctggt caggaacagc
    28201 cggacagcgg caccatcact ttaggtgaaa cggtgaaact ggcgtcggtt gatcagttcc
    28261 gtgactcaat ggataacagc aaaaccgttt gggaagaagt ttccggcggg ctggatatta
    28321 tgaagatcgg caacaccgag atgccaagcc gcgcctacgt tggccgcttt aactttaaag
    28381 gggttgatca gggtaaacgc gttggtgaac tttccggtgg tgagcgcggt cgtctgcatc
    28441 tggcgaagct gctgcaggtt ggcggcaaca tgctgctgct cgacgaacca accaacgacc
    28501 tggatatcga aaccctgcgc gcgctggaaa acgccctgct ggagttcccg ggctgtgcga
    28561 tggttatctc gcacgaccgt tggttcctcg accgtatcgc cacgcacatc ctggactacc
    28621 aggatgaagg taaagttgag ttcttcgaag gtaactttac tgagtacgaa gagtacaaga
    28681 aacgcacgct gggcgcagac gcactggagc cgaagcgtat caagtacaag cgtattgcga
    28741 agtaatgcgt aaaatgccgg atgcggcgtg aacgccttat ccggcctaca aaccaagaaa
    28801 tttcaatacg ttgtgaatct ccaggcctga taagacgcgg taagcgtcgc atcaggcatt
    28861 gagcaccgaa tgccggatac ggcgtgaacg ccttttccgg cctacaacag ccttagtatc
    28921 cgcaatttcg tagcgggtca cccgtgcagg ttttttcaaa ccttgtttaa tctcgacagc
    28981 ctcttccaga gaggttttca actcatcaaa aaacttattt tatacctcgc ctttgagccg
    29041 tttcatcgcg gttatctctg ctcccccatc atctcccgca ccagctccac gcagcgcagg
    29101 aaacggctgt cataatcgtc ctcttcaacc cgcacgaatt cgatattgtt ctcttccagc
    29161 atctccacca gcaagttctg gaactctttg cgatccaccg aactgccgag gctgcgtaaa
    29221 ccatccgcca cccacggcgt gttgttctcc agcaggatca ccagatcgaa acggtattca
    29281 tcaatcaacg cctgtacgaa cggatgctca cgcccttcgt actttttgca gaacgcctgg
    29341 gtagtgacaa aatcagtgtc aataaacgcc actttattgg catatttcac tgcaaaatca
    29401 atgtattgtg cgtggcccag cgcgatttta tcgtaatcgg aatactgcaa tgcgatctca
    29461 tcaccgccga ggtgtgaaaa gacataatcg cgaccatatt cccacgcact ggtggtattg
    29521 aagatattgg caagtttgtt taccagggtg gatttaccgc tcgactcgcc accaaggatc
    29581 gccacggtac gtacaaagaa cggcttcact tccgtaggaa tatattccca gtagcggaac
    29641 gggttttcgc ggatctgcgc accgctgata ctcataaagg tacgtttcgg atcgaccagc
    29701 accgtatcga tccccagatg ttccatatac tgtggcgcat cggcttcttc cgaggtgtag
    29761 atcaggtccg gctgaatccc cttttcagcc ataaactttt tgatgccgtt gctccacaca
    29821 tcccagccgt gcggatacgg ctccatgccc tcttcgttga aagcatgaat gcgaatattt
    29881 ttctgatatt taaaagtttg taataaccag cgcagacgat ccggcaccgt gggctgctgc
    29941 gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat cgtcaaaacc cataatgata
    30001 tgcagctcgt caacctggct acaggcgcgc tggataaggt agatatgtcc ggtatgcagc
    30061 gggtagaact taccgaatac gacaccaatg gttttcttct gtcggggaaa ttcaagcccc
    30121 aaaaaacggt gcaacgcctc cagcttttgc gcgctggggc ttttaatttt ggcattcagt
    30181 aactggctta aatacccttt ggtcataccg ctggcatcag ctacctgctg tagcgtgcag
    30241 ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg acatatctcc ctccgtatct
    30301 ctcattataa gtcgtcgaac acgctaagcg cgtcggagag ttttttaacg ccaaaaatct
    30361 gcatcccttc cggcgctttt ttcggtacgt tagccgccgg aacaatcgcc cggcgaaaac
    30421 cgtgtttcgc cgcttctgaa attcgttcct gaccgctggg caccgggcgg atctcccctg
    30481 ccagcccgac ttcaccaaac accaccagat cctgtggcag cggtctgtca cgcaggctgg
    30541 aaaccatcgc cagcagtaac gctaaatcgg cactggtttc ggttaccttc acgccgccga
    30601 ccacgttcac aaatacatcc tgatcggcca tttgcagacc accgtgacgg tgcaagacag
    30661 ccagcaggat tgccagacgg ttttgttcca gccccactgc cacgcggcgc ggattcgcca
    30721 tcatcgagtg atcgaccagc gcctgaatct ccaccagcaa cggacgcgtt ccttcccaca
    30781 ccaccatcac ggagctgccg gaggtcactt catctccgcg acttaagaaa attgccgaag
    30841 ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa gacgcccagc tcattcaccg
    30901 cgccgaagcg gtttttatgg ctgcgcaagg tgcgaaaacg ggagtcggca tcaccatcca
    30961 gaagcaccga acagtcgata cagtgttcca gcactttagg gccagccagc gagccatctt
    31021 tggttacgtg cccaaccatg acaatcgcca caccgcgcgt tttggcgaag cgcgtcagat
    31081 aagccgccgt ttcacgcacc tgcgccacgc taccaggcga tgattgtaca tccgccatat
    31141 gcatcacctg gatcgagtca attaccatca gtttcggttg ctcttcttcg gcaatcaggc
    31201 agatctgttc gatgctggtt tccgacaaca tattgagatt gtcagtcggc aggccaaggc
    31261 gatgagcgcg cattgccacc tgttgcagcg actcttcgcc agtgacatac agcgttttca
    31321 tctgctgggc cagtttgcac agcgtttgca gtagcagcgt ggatttcccc gcacctgggt
    31381 taccgccaat cagaatggcg cttcccggca ccacgccgcc gcctagtacg cggtcgaact
    31441 ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct gatatcggag agtttctgga
    31501 cttttgccac cccggcgcta ccggcatagc cgctgagacg ctcgttacgc gccaccgttg
    31561 gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc atgacaggca ctgcactgcc
    31621 cctgccagcg cggataatcg gccccgcatt cattacaaac aaaggcgcgt tttggagctt
    31681 ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg atggcgggcg agcaattact
    31741 tctgattcag gctgccagag aggatgcaga atacccccat caggtcagcg tgacggatgg
    31801 tgacttccgc cttttcattc acttttggct tggcatggta ggcaatcccc agccctgctg
    31861 ctttgatcat cggcaggtca ttagctccat cgccaatcgc cacggtctgc gccagcggga
    31921 tttcatactc ctgcgcgagg cgagtcaaag ttttcgcttt gtactgcgcg tctacgatgt
    31981 cgccaatcac attgccggta aatttaccgt ccatgatctc cagttcattg gctaccacgg
    32041 cggtcaggcg cagcttgtcg cgcagatatt cagcaaagaa agtaaagccg ccggaggcaa
    32101 tcgccacttt ccagcccagc gtttccagct tgagcaccag ttgcgttaag cctggcatca
    32161 gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc agcgcctttc agcgtcgcca
    32221 cacggctgcg caggctggcg gtaaaatcga gttcgccgcg catcgcccgt tcggttactt
    32281 ccgccaccat ctcgcccgtt ccggccagtt tggcaatttc atcaatacat tcaatctgga
    32341 tggcggtgga atccatgtcc atcaccagca aacccggcgt gcgcaggtgc gggattttcc
    32401 ccagcggggc gacatccagt tgcgcttcat gggccaggcg cgtagcccgt gcggtgagtg
    32461 aacctgccag acgaatcacc tgataatctt ccacgcacca ggcggcaaca atcaccatcg
    32521 ccgcacccag tttgctctgg tattgggtca gacgctgttt atccagccca cgaccataca
    32581 gcagccagcc gctacgacct gcgtggtaat ccagtggcat cacttcatca ccacttaatg
    32641 aaagaggcag acccggccat aaagagacat cttcaggcag gtcgcaccag gtaatgttag
    32701 gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat aacgcatgag gctaccttgt
    32761 atccattgct tctggcaaca ttaagtctca aattttcaaa gggtggaaga tggctcgcac
    32821 aaaactgaaa ttccggctgc atcgggcagt gattgtcctg ttctgtcttg ccttgttagt
    32881 ggcactgatg cagggggcgt catggtttag tcaaaaccac cagcgacagc gtaatccaca
    32941 gctggaagaa ctggcccgca ccctggcgcg tcaggtgacg ctgaacgttg caccgctgat
    33001 gcgtaccgac tcaccagatg aaaaacgcat tcaggcgatc ctcgatcagt taacggatga
    33061 aagccgcatc ctcgacgcgg gtgtgtatga cgaacaaggc gatcttatcg cccgttcagg
    33121 cgaaagcgtc gaagtgcgcg accggctagc gctcgacggt aaaaaagcag gcggctattt
    33181 taaccagcag attgtcgagc caattgcggg taaaaacgga ccgctcggct atctgcgcct
    33241 gacactcgac acccatacgc tcgccaccga agcccaacag gtggataaca ccactaacat
    33301 tttacgcctg atgttgctgc tctcactggc aatcggcgta gtgctgaccc gcacgctgct
    33361 acagggtaaa cgcacccgct ggcagcaatc gcccttcctg ttaaccgcca gcaaaccggt
    33421 gccggaagag gaagaaagcg agaaaaaaga gtgacccatt actacaagaa aggaaatcgt
    33481 tatgtccaca ttacgcctgc tcatctctga ctcttacgac ccgtggttta acctggcggt
    33541 ggaagagtgt atttttcgcc aaatgcccgc cacgcagcgc gttctgtttc tctggcgcaa
    33601 tgccgacacg gtagtgattg gtcgcgcgca gaacccgtgg aaagagtgta atacccggcg
    33661 gatggaagaa gataacgtcc gcctggcgcg gcgcagtagc ggtggcggcg cggtgttcca
    33721 cgatctcggc aatacctgct ttacctttat ggctggcaag ccggagtacg ataaaaccat
    33781 ctctacgtcg attgtgctca atgcgctgaa cgcgctcggc gtcagcgccg aagcgtccgg
    33841 acgtaacgat ctggtggtga aaacagtcga aggcgaccgc aaagtctcag gctcggccta
    33901 tcgcgaaacc aaagatcgtg gcttccacca cggcactttg ctgctcaatg ccgaccttag
    33961 ccgcctggca aactatctca atccggataa aaagaaactg gcggcgaaag gcattacctc
    34021 agtgcgttcc cgcgtgacca acctcaccga gctgctgccg gggatcaccc atgagcaggt
    34081 ttgcgaggcc ataaccaagg cctttttcgc ccattatggc gagcgtgtag aagcggaaat
    34141 catctccccg gacaaaacgc cagacttgcc aaacttcgcc gaaacctttg cccgtcagag
    34201 tagctgggaa tggaacttcg gtcaggctcc ggcattctcg catctgctgg atgaacgctt
    34261 tagctggggc ggcgtggaac tgcatttcga cgttgaaaaa ggccatatca cccgcgccca
    34321 ggtgtttacc gacagcctca accccgcgcc gctggaagcc ctcgccgggc gactgcaagg
    34381 ctgcctgtac cgcgcggata tgctgcaaca agagtgcgaa gcgctgttgg ttgacttccc
    34441 ggaccaggaa aaagagctac gggagttgtc gacgtggata gcgggggcgg taaggtaact
    34501 cgctgattaa aataccaaag gaagggaata tgatcgatct ggaaaaccag gagcgggaga
    34561 ttatcaattt aatgttaagc cagcgtattt cctggctagc cgccgtgcgt atcaggcata
    34621 aattatcact tgctgaagta agtaaaatgt taggtatctc gattaattct ctaaaataga
    34681 tcgaaaagac ggaacggctt agctcaaaca tcaaaagcaa aatgggagaa atttatggtt
    34741 gtccaccaga gttgctgata tgcccttcct ggatgacggc tgaacataaa tgaatagatt
    34801 cgtgaataga tacttgaatg tattttattt tttatccata tgaaaaataa tagtaaatct
    34861 ataaatcagt tgctattgat gaatagatac taaaaagccg gagcagtccc ccactccggc
    34921 ttaacacgac aatcgccttt cagcgaaaca caattactct ttatcgccca gcagaacgga
    34981 ttccagtgcg attttgatca tgtcgttgaa ggtggtctga cgctcagcgg cagtggtctg
    35041 ctcgtgagtg cggatgtggt cagacacagt gcagatggtc agggctttcg cgccaaattc
    35101 tgcagcgacg ccgtagatac cagccgcttc catttccacg ccgaggatgc cgtatttttc
    35161 catcacgtcg aacatttcgc cgtccggaga gtagaacagg tcagcggaga acaggttacc
    35221 aacgcgagca tcaatgccca gtgctttagc cgcgtctacc gcgttacgca ccatgtcaaa
    35281 gtcagcgata gcggcaaagt catgatcttt aaaacggatg cggttaactt tggaatcggt
    35341 gcaggcaccc ataccgataa cgacgtcgcg cagttttacg tgcggcagaa ctgcgccaca
    35401 ggaacccacg cggataattt tcttcacgcc gaaatcggtg atcagttctt tggtgtagat
    35461 ggagcaggac gggataccca taccgtgacc cattacggaa attttgcggc ctttgtaagt
    35521 accggtgaag cccagcatac cgcgaacgtt gttcacttca cgggcatctt caaggaaagt
    35581 ttcagcaata tacttcgcac gcagcgggtc gcctggcatc aaaactacgt cagcgaaatc
    35641 gcccatttct gcattaatgt gtggggtagc cattgtttta tccttatcaa catgttttca
    35701 ccctctccct gagggagagg gccggggtga gggcatcaag ccgcacccat tatcagaaca
    35761 tggctttgcc atattccata tcagaagtac caaaatattt tgccagagtc tggccgatat
    35821 ccgcgaaggt ttcacggtga cccagtgagc ccggttttac tttcgggccg taaaccagta
    35881 ccggaatgtg ttcacgcgtg tggtcagtac cggtccaggt cggatcacaa ccgtggtcag
    35941 cagtgaggat caggatgtcg tcatcgcgca gcagagacat cagctccggc agacggcggt
    36001 cgaacagctc cagacccaca gcataaccgg cgacgtcgcg acggtggccc caggaagagt
    36061 cgaagtcaac gaagttggtg aagacgatgg tgttatcacc cgcttctttc atctctttga
    36121 tggtggcgtc aaacagcgcg tccaggccag ttgctttcac tttcttggtg ataccgcagt
    36181 tggcgtagat gtctgcaatt ttaccgacag aaaccacctg accgtgtttt tcatcaacca
    36241 gtttctgcag cacggtcggt gctggcggct caacagccag gtcgtgacgg ttaccggtac
    36301 gctggaagtt accggctttg tcaccgataa acggacgagc gataacacga ccgatgttgt
    36361 agccgccgtt ggtcagctct tcacgggcga tttcgcacag ttcgtagagt ttatccaggc
    36421 caaaggtttc ttcgtggcag gcaatctgga acacagagtc agcggaggta tagaaaatcg
    36481 gcttgccggt tttcatgtgc tcttcgccca gctgatccag aatgaccgta ccggaagagt
    36541 ggcagttacc gaggtaaccc ggcagattag cgcgttcaac cagtttatcc agcagctctt
    36601 gcgggaagct gttttcgtga tcggagaaat atccccactc aaatagaacc gggacaccgg
    36661 ctatttccca gtgaccagac ggggtatctt taccggatga catttcgtgc gcccatgcgt
    36721 acgcgccgat aacttcagcg ttgccgtcca ttcccgccgg aatgaaaccg gtagaacctt
    36781 cgtgtgcttt cgccagcccc agacgggtca gatttggcag attgagcgga cctttacgac
    36841 cgttatcagc ttcgcctttg gcacaagctt ctgcgatatg acccagggtg tcagccccga
    36901 cgtcaccaaa gcgttctgca tcttctgtag cgccgatgcc gaatgagtcc agcaccataa
    36961 taaatgcacg tttcatatgt tctccgtact cagtgcttca aatttaaatg atcagatcag
    37021 tataccgtta ttcgctgata cggcgataga cagttggtgt gctttccggt gctttatcgg
    37081 caagtttaat tgccgctttc accgctttcg ccgcttcctg ccagctgttt tcgtctttcg
    37141 cgtggataac cgccagcgga cgctgaccgt ctacctggtc gcccaaacgc gccatatcag
    37201 taaagccgac gctgtaatca atggtgtcag atgcctgacg acgaccgccg cccattgcaa
    37261 ccactgccat ccccagcgcg cgggtatcca tttcactgac aaaaccttcg gtatcagcat
    37321 agactgcttt cgtcagcatc gctgtcggca gatacttcgc gtagttctca acgaagtcgg
    37381 ttgggccttt ttgtgccgct accatacgac caaagacttc tgccgcttta ccgttgtcca
    37441 gcactgcttg caatttcgcg cgcgcttcgg cgtcatcttt cgccagtttg ccggagataa
    37501 gcatctccac gcacagcgcc atcgtgacat caaacagacg cgggttacga tactcacccg
    37561 tcaggaactg caccgcttca cgaacttcaa ccgcgttacc tgcactggag gccagtacct
    37621 gattcatgtc ggtgagcagc gcggtggtgc gcacgccagc gccgttagcc acgccaacaa
    37681 tcgcttcggc aagggcttca gagagttcgt aggtcggcat aaacgcgccg ctacccactt
    37741 tcacgtccat caccagcgcg tccagacctt ccgcaagttt cttcgccaga atagaggcgg
    37801 tgatcagcgg gatggagtcc acggttgcgg taatatcacg ggtcgcgtag aaacgtttat
    37861 ccgccggagc cagtgagctg gtctgaccga taatcgccac gccgacgtct ttaataattt
    37921 cgcggaaacg gttgtcatcc gggaaaatgt cgaagccagg gatggattcc agtttgtcga
    37981 gcgtaccgcc agtatgaccg aggccgcgac cagagatcat cggaatatag ccgccgcagg
    38041 ctgcgaccat cggccccaac atcagcgaag tcacatcgcc gacgccacca gtggaatgtt
    38101 tatcaacaat cgggccattc agatgcaggc ttttccagtc gagaacggtt cctgaatctc
    38161 gcatcgccat ggtcagcgag acacgctcag gcattgtcat atcgtggaag aaaatggtca
    38221 tcgcgagggc ggcaatctgc ccttcggaga tagtgttgtc gcgaatgccg ttgataaaga
    38281 aacgaatttc ttcatcgctc agtgcatgac catcacgttt tttacgaata atttcttgtg
    38341 cgagaaacaa ggtaacctcc cgaggaaaag agtatgaggc ggcttttgcc ggatgcgtct
    38401 acgccttatc cggcctacgg gtagatacgt ctcgtaggcc tgataagcgc agcgcatcag
    38461 gcgtaaagca tcttacttag tagctgctgg cgctcttacc gtcaccgtgg cccagcgctt
    38521 tcaacaggct tgccagcagg ctggaagcac caaagcggta gtgacgcgca tctgcccagt
    38581 cagcaccgaa cagttcatct gcgatggcga gatatttctg cgcatcttcc gcagtacgca
    38641 cgccgcccgc tggtttgaaa ccaacggttt tttctacgcc catatcacgg atcacttcca
    38701 tcatgatgcg cgcgctttcc ggcgtcgcgt tcacagccac tttaccggta gaggttttga
    38761 tgaagtccgc acccgctttg atggagattt cagacgcttt acggatcagc gcttcgtctt
    38821 tcagttcgcc ggtttcgatg atcactttca gcagtacatt cgctgccgcg caagcctctt
    38881 tacaggcttt caccaggtca aaaccaacct gctcgttacc cgccatcagc gcgcggtacg
    38941 ggaacacaac gtcaacttca tcagcaccgt aggcgattgc cgcacgggtt tctgccagcg
    39001 cgatttcgat gtcatcgtta ccgtgtggga agttggttac cgtagcgatt cggatttccg
    39061 gggtgccctg ctctttcagc gttttgcgag caatcgggat aaagcgagga tagatacaga
    39121 tagcggcggt attgccgacc ggggttttgg cctgatgaca cagagcaatt actttctcgt
    39181 cggtgtcgtc gtcattcagg gtggtcaggt ccatcaattt cagtgcacgc aggctgcttg
    39241 ctttcagatc agtcatttca ttctcctggc ttgtcgccaa taaaattcac cttgcgagtt
    39301 tgttagtatt ctaacatcta ctccgcaaca cacttcgata tacatcacaa ttaaggaaat
    39361 ctacttacaa gtttgcatca ctgtaatgcg atctggttca aataattcac tttcaaatga
    39421 atgcgtcagt ggtggcaaac gcatcaggat caaagtgaac atcacgaaac ttcttacaat
    39481 ggcgcatcac cttcggcata aaaggaaaag aagatgccca cttctcatga aaatgcactg
    39541 caacaacgtt gccagcaaat tgtcaccagc ccagtgctta gcccggagca gaagcgccat
    39601 tttctggcac tggaagcaga aaacaatctg ccttatccgc agcttcctgc cgaagcccgc
    39661 cgcgcgctgg atgagggtgt aatctgcgat atgtttgaag gtcatgcgcc gtacaaaccg
    39721 cgctatgtct tacccgatta cgcccgtttt ctggcgaacg gttccgaatg gctggagctg
    39781 gaaggcgcga aagatcttga tgacgcactc tctctgctga ccattcttta ccaccacgta
    39841 ccgtcggtca catcgatgcc ggtctacctg gggcaactgg atgcgttgtt gcaaccatat
    39901 gttagaattc taacacaaga cgagatcgat gttcgaataa aacgtttctg gcgttacctc
    39961 gacagaaccc tgccagacgc ctttatgcac gccaatatcg gcccgtctga ttcgcccatt
    40021 acccgcgcga tcttacgtgc agacgcagag ttgaagcagg tttcaccgaa cctgaccttt
    40081 atctacgatc ctgaaatcac ccctgatgac ctgctgctgg aagtggcgaa gaacatctgt
    40141 gaatgtagca aaccgcacat cgccaacggt ccggtgcatg ataaaatttt cacaaaaggg
    40201 ggctacggga ttgtgagctg ttacaactca ctgccgctgg cgggtggtgg cagcacgctg
    40261 gtacgcctta acctgaaagc cattgccgag cgcagcgaat cgctggatga cttctttacg
    40321 cgcactctac cgcactactg ccagcagcag atcgccatca tcgatgcgcg gtgtgaattc
    40381 ctctatcaac aatcacactt ctttgagaat agcttcctgg tgaaagaagg gctgattaac
    40441 cctgaacgtt ttgtgccaat gtttggcatg tatgggctgg cggaagcggt taacttgctg
    40501 tgtaagaaag aagggattgc cgcgcgctac ggtaaagaag ccgccgcaaa tgaagtgggt
    40561 tatcgcatca gcgcgcaact ggcggagttt gtcgccaata cccccgtgaa atatggctgg
    40621 caaaaacgcg ccatgttaca cgcacagtcg gggagcagtt ccgatatcgg caccacgccg
    40681 ggcgcgcgtt tgccgtatgg tgatgagcca gatccgatca cccatctgca aactgtcgcc
    40741 ccgcatcatg cttattatta ttccggcatc agcgacattc tgacgctcga cgaaaccatc
    40801 aaacgtaatc cgcaggcgct ggtacagctt tgcctcggtg cctttaaagc cggaatgcgt
    40861 gaatttactg ccaatgtcag cggtaacgat ctggttcgcg ttaccggtta tatggtgcgt
    40921 ttgtcggatt tagaaaaata tcgcgccgaa ggttcacgca ccaacaccac ctggctgggc
    40981 gaagaagccg cacgcaacac tcgtattctg gaacgccagc cgcgcgtgat aagccatgaa
    41041 cagcagatgc gctttagtca gtaagattat ccccttctcc tgcgttgacg ggccaggcag
    41101 tcgtctggct ctgtttttgc agggctgcaa tctgcgctgc aaaaactgtc acaatccgtg
    41161 gacgatggga cgttgcaatg actgtgggga gtgcgtgcca cagtgtccgc atcaggcgtt
    41221 gcagattgtt gacggcaaag tggtgtggaa cgctgtggtt tgcgagcagt gtgatacctg
    41281 cctgaagagg tgtccgcaac atgccacgcc aatggcgcaa tccatgagcg tggacgaagt
    41341 gcttagccat gtccgcaaag cggtgctgtt tattgaaggg ataacggtaa gcggcggcga
    41401 agccacgacc caactgccgt ttgtagtggc gctgtttact gctatcaaaa acgatccgca
    41461 actgcgccac ctcacctgcc tggtggacag taacggcatg ttgagcgaaa ccggctggga
    41521 aaaattactc ccggtgtgtg acggcgcaat gctcgatctc aaagcgtggg ggagcgaatg
    41581 tcatcaacac ttaaccggac gcgataatca gcagattaag cgcagcatct gtttgcttgc
    41641 agagcgcggc aagctggcgg aactgcgttt gctggtaatt ccagaccagg tggattattt
    41701 gcatcacatc gatgagctgg cgacgtttat caagagactt ggcgatgttc cggttcgcct
    41761 gaatgcgttt catgcccacg gcgtgtatgg cgaggcgcaa agctgggcga gcgccacgcc
    41821 ggaagacgtt gagccgttgg ctgatgcgtt aaaggtgcgc ggggtgagcc ggttgatatt
    41881 tccggcgctc tatttgtgac atgccggata cggcctacgg ttcggcacag acttgtaggc
    41941 atgataagac gcgacaagcg tcgcatcagg cattatgcgc cgactgccgg atgcggcgtg
    42001 aacgccttat ccggcctacg gttcggcaca gacttgtagg catgataaga cgcgtcagcg
    42061 tcgcatcagg cattgtgcgc caactgccgg atgcggcgtg aacgccttat ccggcctacg
    42121 gttcggcaca gact
//
LOCUS       DAJVRF010000016        40888 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJVRF010000016 DAJVRF010000000
VERSION     DAJVRF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40888
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.016"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            61..741
                     /locus_tag="OQN13_000814"
                     /pseudo
     CDS             61..741
                     /locus_tag="OQN13_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
     gene            743..979
                     /locus_tag="OQN13_000815"
     CDS             743..979
                     /locus_tag="OQN13_000815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614259.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAALYCVAKIF
                     PDGENILIYKVNIINASSKKDVFFMKAQHQPTMT"
     gene            complement(1152..1827)
                     /locus_tag="OQN13_000816"
                     /pseudo
     CDS             complement(1152..1827)
                     /locus_tag="OQN13_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1972..2580)
                     /locus_tag="OQN13_000817"
     CDS             complement(1972..2580)
                     /locus_tag="OQN13_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCS1614260.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYPPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(2764..3081)
                     /gene="metJ"
                     /locus_tag="OQN13_000818"
     CDS             complement(2764..3081)
                     /gene="metJ"
                     /locus_tag="OQN13_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCS1614261.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            3358..4518
                     /gene="metB"
                     /locus_tag="OQN13_000819"
     CDS             3358..4518
                     /gene="metB"
                     /locus_tag="OQN13_000819"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCS1614262.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4521..6953
                     /gene="metL"
                     /locus_tag="OQN13_000820"
     CDS             4521..6953
                     /gene="metL"
                     /locus_tag="OQN13_000820"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCS1614263.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            7302..8192
                     /gene="metF"
                     /locus_tag="OQN13_000821"
     CDS             7302..8192
                     /gene="metF"
                     /locus_tag="OQN13_000821"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCS1614264.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            8521..10701
                     /gene="katG"
                     /locus_tag="OQN13_000822"
     CDS             8521..10701
                     /gene="katG"
                     /locus_tag="OQN13_000822"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCS1614265.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            10795..11700
                     /gene="yijE"
                     /locus_tag="OQN13_000823"
     CDS             10795..11700
                     /gene="yijE"
                     /locus_tag="OQN13_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCS1614266.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(11727..12344)
                     /locus_tag="OQN13_000824"
     CDS             complement(11727..12344)
                     /locus_tag="OQN13_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCS1614267.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(12618..13721)
                     /gene="gldA"
                     /locus_tag="OQN13_000825"
     CDS             complement(12618..13721)
                     /gene="gldA"
                     /locus_tag="OQN13_000825"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCS1614268.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(13732..14394)
                     /gene="fsa"
                     /locus_tag="OQN13_000826"
     CDS             complement(13732..14394)
                     /gene="fsa"
                     /locus_tag="OQN13_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCS1614269.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(14406..16907)
                     /gene="ptsP"
                     /locus_tag="OQN13_000827"
     CDS             complement(14406..16907)
                     /gene="ptsP"
                     /locus_tag="OQN13_000827"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1614270.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            17216..18295
                     /locus_tag="OQN13_000828"
     CDS             17216..18295
                     /locus_tag="OQN13_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCS1614271.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTGGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            18310..18630
                     /locus_tag="OQN13_000829"
                     /pseudo
     CDS             18310..18630
                     /locus_tag="OQN13_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            18681..20977
                     /locus_tag="OQN13_000830"
                     /pseudo
     CDS             18681..20977
                     /locus_tag="OQN13_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            20943..21821
                     /locus_tag="OQN13_000831"
     CDS             20943..21821
                     /locus_tag="OQN13_000831"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCS1614272.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            21823..22164
                     /locus_tag="OQN13_000832"
     CDS             21823..22164
                     /locus_tag="OQN13_000832"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCS1614273.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(22151..23002)
                     /locus_tag="OQN13_000833"
     CDS             complement(22151..23002)
                     /locus_tag="OQN13_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1614274.1"
                     /translation="MYHGVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(23217..24950)
                     /locus_tag="OQN13_000834"
     CDS             complement(23217..24950)
                     /locus_tag="OQN13_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCS1614275.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(25133..27784)
                     /gene="ppc"
                     /locus_tag="OQN13_000835"
     CDS             complement(25133..27784)
                     /gene="ppc"
                     /locus_tag="OQN13_000835"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCS1614276.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(28136..29287)
                     /gene="argE"
                     /locus_tag="OQN13_000836"
     CDS             complement(28136..29287)
                     /gene="argE"
                     /locus_tag="OQN13_000836"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCS1614277.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPSMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            29441..30445
                     /gene="argC"
                     /locus_tag="OQN13_000837"
     CDS             29441..30445
                     /gene="argC"
                     /locus_tag="OQN13_000837"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCS1614278.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            30453..31229
                     /gene="argB"
                     /locus_tag="OQN13_000838"
     CDS             30453..31229
                     /gene="argB"
                     /locus_tag="OQN13_000838"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCS1614279.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            31290..32663
                     /gene="argH"
                     /locus_tag="OQN13_000839"
     CDS             31290..32663
                     /gene="argH"
                     /locus_tag="OQN13_000839"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCS1614280.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALSVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            32930..33847
                     /gene="oxyR"
                     /locus_tag="OQN13_000840"
     CDS             32930..33847
                     /gene="oxyR"
                     /locus_tag="OQN13_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCS1614281.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPIADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(33830..35230)
                     /gene="sthA"
                     /locus_tag="OQN13_000841"
     CDS             complement(33830..35230)
                     /gene="sthA"
                     /locus_tag="OQN13_000841"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCS1614282.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            35551..36201
                     /gene="fabR"
                     /locus_tag="OQN13_000842"
     CDS             35551..36201
                     /gene="fabR"
                     /locus_tag="OQN13_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCS1614283.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            36201..36560
                     /locus_tag="OQN13_000843"
     CDS             36201..36560
                     /locus_tag="OQN13_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCS1614284.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(36600..37700)
                     /gene="trmA"
                     /locus_tag="OQN13_000844"
     CDS             complement(36600..37700)
                     /gene="trmA"
                     /locus_tag="OQN13_000844"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCS1614285.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      37814..38004
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            38069..39913
                     /gene="btuB"
                     /locus_tag="OQN13_000845"
     CDS             38069..39913
                     /gene="btuB"
                     /locus_tag="OQN13_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCS1614286.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRGTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            39858..40715
                     /gene="murI"
                     /locus_tag="OQN13_000846"
     CDS             39858..40715
                     /gene="murI"
                     /locus_tag="OQN13_000846"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCS1614287.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9451 a        10184 c        11229 g        10024 t
ORIGIN      
        1 tataaccacc acagccagtt aacgtcagcc accgggcctg acgggctgga aatacgccgg
       61 gaatatgatg aatggggcaa cctgctgaat gaagagaacc cgcatcagct gcagcagctt
      121 attcgtttgc cggggcagca gtatgatgag gagtccggcc tgtattataa ccgccaccgc
      181 tattatgacc cgctgcaggg gcggtatatc actcaggatc cgattgggct gaagggggga
      241 tggaacctgt atacatatcc gctgagcccg gtgaatagca tggatccatt aggattatat
      301 gaatttaaat caaaaaatat tgatgatatt ggaatatttg cattggcaat gtgtaatgga
      361 gaatcaatta acgagaataa agaatatggt ggactaatat gtaagaagca aggtgaatat
      421 ttccccatga atccgataag ttcaaatgat aatgatagtg tagacttgcg aaatataaaa
      481 tgccctgaag gttcagagag agtaggcgat tatcacactc acggttttta ctctgacgat
      541 aaaggaaata aagtaacaaa agaaaatgat gtttatgata gtctaaattt ttcaagcaaa
      601 gatttaacga attcttatat gaatggaatg ggaaaaaaag aatacagtag ttacttggga
      661 acaccaaata acacctatct aaaatataat cccaaagcta aagggaatgg agttacaatt
      721 atcaggcaag ggagtaatta aaatgaaggc aatacaatgt tgttatttcc ttgctttagt
      781 ctccagtcat ttaatggctg gagttatggg tgatgataat atatttttaa gtaattcgga
      841 ttcagctgct ttatattgtg tggctaaaat atttccagat ggagaaaata ttttaatata
      901 taaagtaaat ataattaatg ctagttcaaa aaaagatgtt ttttttatga aagcccaaca
      961 tcaacccaca atgacataat ggggggcggg ggaatgtcat atgcctatga atatgatgta
     1021 aaatataata tatgtcattt gagaaaaaag agcagaatga ggtaaaaatc tttggctgtg
     1081 tattcagtct ggtaatcatc caaggaaagc ttatcagaag ggtgatgact ccaattagta
     1141 gaacatatat tttcgataaa agcaccgatg actttttcat gaagttctac agaacgcgag
     1201 aaacagatgg ttctgcgggc cagacgtttg attcgggttc tcagggtcag attgttacgc
     1261 tcaatgtgct gagtaaaaat cttaccagtc agatgcttct ccttcggtaa ctctctagcg
     1321 taactacccc attcgtcagt ggtcaacatg ccgatggtaa aagggtaagc agcgcccgca
     1381 attcacgaca ggtttcgtca gtccgggggc caaaagtgta ggccagaaca ccaccagttt
     1441 tcgtgttgta cacgtaccag agccagtgct gtcgcgcttt actgccgaca aagctccatt
     1501 gctgactaag tttgcagatg agtgccacat cagcatgagc gaccggggat gacgttattc
     1561 ttcgtggcga gagtttttta aagttcggat gactgtatta atgccgattt tcagcgtacg
     1621 ggcagtatcg cgaaccccgg caccgttgaa agtcatttca gcgatttggt ctttaactcc
     1681 cggtttacgg ggctcgtaac agtaactgag ctgaaatacc cggtgacagt cacggccgcg
     1741 gaatcgctca tgtctttttg ggttctgccc gtgacggtaa acctgaacgg actggcaacg
     1801 ggggcaatga aggttaactg tgaccatgaa acaaccttaa aagcgagtat tatatcctta
     1861 attcaactaa ttggaggcat caccgtttac cggagcgaca gcttgcggaa aggtcgtgca
     1921 actacaaagc aacaaacaga ctacggcggg aattattttt ttcatcatcc attaccacga
     1981 aaacagcggc cactctttgg cgactgtggt gagttgtggc gcgtcgaaaa cggcttgtgg
     2041 tgaaactacc gtttcctcaa gcggaatatt tttgccgtag cgttctttga gcttagcctg
     2101 gaccagcggg ttgctgagat cgaactcttt caacttctgc tgctcgccca cgcgcattcc
     2161 cagcgcgttc tggtaaacct tccacaccac ttccgaacag tactggcgat cgtcgctcca
     2221 tgagaagctg aaatcatacg gtttaccgag ataacgtttt gccgtttgcg ccagtttttg
     2281 ctgttgttca acactcagtc cgccttcaac gcggcgaaca acgtatttgc ccttttcacc
     2341 atgggcgatc cactgcttga gcggggggta tttcaccggg ccgaccgctt caaaaacgta
     2401 gggctttttg ttgcgtatca ccagcatacc ggtgtggcta taatcggaat gggtcgccag
     2461 ttggatcgct ttactttgcg atgagcgaga gatctgaaag atgatgtcgc cggtttgtgg
     2521 ctgccaggca aaggcaggta cagaaaccag caggctgagg atcagcagcc tgtttttcat
     2581 agttaaacgt ccatgtataa aaagcggtgg gtcgcagaca acgtgctcgt tgtttatgcc
     2641 ggatgcggcg tgaacgcctt atccggccta caagttcgtg caaattcaat aaattgcaat
     2701 atgacgtagg cctgataagc gtagcgcatc aggcgattcc actccgcgcc gctctttttt
     2761 gctttagtat tcccacgtct ccgggttaat ccccatctca cgcatgatct cttttgccgc
     2821 ttccgggatt tcgtcgctgc gctctttacg cagatcggca tcatccggca aaggttgccc
     2881 ggtaaaggca tgcagaaacg cttcgcacag cagctcgctg ttggtagcgt gacgcaggtt
     2941 gttcacctga cgacgcgtgc gttcatcggt gaggattttt aacaccttaa gaggaatgga
     3001 aaccgtaatc tttttgactt gttcactctt cttgccgtgc tcagcgtatg ggctgatata
     3061 ttcgccgctc cattcagcca tgagatactt aatcctcttc gtcaataaat tgagaccaga
     3121 ccacacaatt tatgtgggta ctgaccgtaa acccgcatag tttaccgtac aggcgttacc
     3181 gtgacatcgt gtaatgcacc tgtcggcgtg ataatgcata taattttaac ggctatttgg
     3241 gatttgctca atctatacgc aaagaagttt agatgtccag atgtattgac gtccattaac
     3301 acaatgttta ctctggtgcc tgacatttca ccgacaaagc ccaggaaact tcatcacatg
     3361 acgcgtaaac aggccaccat cgcagtgcgt agcgggttaa atgacgacga acagtatggt
     3421 tgcgttgtcc ctcccatcca tctttccagc acctataact ttaccggatt taatgaaccg
     3481 cgcgcgcatg attactcgcg tcgcggcaac ccaacgcgcg atgtggttca gcgtgcgctg
     3541 gcagaactgg aaggcggtgc tggcgcagta cttactaata ccggcatgtc cgcgattcac
     3601 ctggtaacga ccgtcttttt gaaacctggc gatctgctgg ttgcgccgca cgactgctac
     3661 ggcggtagct atcgcctgtt cgacagtctg gcgaaacgcg gttgctatcg cgtgttgttt
     3721 gttgatcaag gcgatgaaca ggcattacgg gcagcgctgg cagaaaaacc caaactggta
     3781 ctggtagaaa gcccaagtaa tccattgtta cgcgtcgtgg atattgcgaa aatctgccat
     3841 ctggcaaggg aagtcggggc ggtgagcgtg gtggataaca ccttcttaag cccggcatta
     3901 caaaatccgc tggcattagg tgccgatctg gtgttgcatt catgcacgaa atatctgaac
     3961 ggtcactctg acgtggtggc tggcgtggtg attgctaaag acccggacgt tgtcactgaa
     4021 cttgcctggt gggcaaacaa tattggcgtg acgggcggcg cgtttgacag ctatctgctg
     4081 ctacgcgggt tgcgaacgct ggtgccgcgt atggagctgg cgcagcgcaa cgcgcaggcg
     4141 attgtgaaat atctgcaaac ccagccgttg gtgaaaaaac tgtatcaccc gtcgctgccg
     4201 gaaaatcagg ggcatgaaat tgccgcgcgt cagcaaaaag gctttggcgc aatgttgagt
     4261 tttgaactgg atggcgatga gcagacgctg cgtcgtttcc tgagcgggct gtcgttgttt
     4321 acgctggcgg aatcattagg gggagtggaa agtttaatct ctcacgccgc aaccatgaca
     4381 catgcaggca tggcaccaga agcgcgtgct gccgccggga tctccgagac gctgctgcgt
     4441 atctccaccg gtattgaaga tggcgaagat ttaattgccg acctggaaaa tggcttccgg
     4501 gctgcaaaca aggggtaaaa atgagtgtga ttgcgcaggc aggggcgaaa ggtcgtcagc
     4561 tgcataaatt tggtggcagt agtctggctg atgtgaagtg ttatttgcgt gtcgcgggca
     4621 ttatggcgga gtactctcag cctgacgata tgatggtggt ttccgccgcc ggtagcacca
     4681 ctaaccagtt gattaactgg ttgaaactaa gccagaccga tcgtctctct gcgcatcagg
     4741 ttcaacaaac gctgcgtcgc tatcagtgcg atctgattag cggtctgcta cccgctgaag
     4801 aagccgatag cctcattagc gcttttgtca gcgatctgga acgcctggcg gcgctgctcg
     4861 acagcggtat taacgacgca gtgtatgcgg aagtggtggg ccacggggaa gtatggtcgg
     4921 cacgcctgat gtctgcggta cttaatcaac aagggctgcc agcggcctgg cttgatgccc
     4981 gcgagttttt acgcgctgaa cgcgccgcac aaccgcaggt tgatgaaggg ctttcttacc
     5041 cgttgctgca acagctactg gtgcaacatc cgggcaaacg tctggtggtg accgggttta
     5101 tcagccgtaa caacgccggt gaaacggtgc tgctggggcg taacggttcc gactattccg
     5161 cgacacaaat cggtgcgctg gcgggtgttt ctcgtgtaac catctggagc gacgtcgccg
     5221 gggtatacag tgccgacccg cgtaaagtga aagatgcctg tctgctgccg ttgctgcgtc
     5281 tggatgaagc cagcgaactg gctcgtctgg cggctcccgt tcttcacgcc cgtactttac
     5341 agccggtttc tggcagcgaa atcgacctgc aactgcgctg tagctacacg ccggatcaag
     5401 gttccacgcg cattgaacgc gtgctggcct ccggtactgg tgcgcgtatt gtcaccagcc
     5461 acgatgatgt ctgtttgatt gagtttcagg tgcccgccag tcaggatttc aaactggcgc
     5521 ataaagagat cgaccaaatc ctgaaacgcg cgcaggtacg cccgctggcg gttggcgtac
     5581 ataacgatcg ccagttgctg caattttgct acacctcaga agtggccgac agtgcgctga
     5641 aaatcctcga cgaagcgggg ttacctggcg aactgcgcct gcgtcagggg ctggcgctgg
     5701 tggcgatggt cggtgcaggc gtcacccgta acccgctgca ttgccaccgc ttctggcagc
     5761 aactgaaagg ccagccggtc gaatttacct ggcagtccga tgacggcatc agcctggtgg
     5821 cagtactgcg caccggcccg accgaaagcc tgattcaggg gctgcatcag tccgtcttcc
     5881 gcgcagaaaa acgcatcggc ctggtattgt tcggtaaggg caatatcggt tcccgttggc
     5941 tggaactgtt cgcccgtgag cagagcacgc tttcggcacg taccgacttt gaatttgtgc
     6001 tggcaggcgt ggtggacagc cgccgcagtc tgttgagcta tgacgggctg gacgccagcc
     6061 gcgcgttagc cttcttcaac gatgaagcgg ttgagcagga tgaagagtcg ttgttcctgt
     6121 ggatgcgcgc ccatccgtat gatgatttag tggtgctgga cgttaccgcc agccagcagc
     6181 ttgctgatca gtatcttgat ttcgccagcc acggtttcca cgtcatcagc gccaacaaac
     6241 tggcgggagc cagcgacagc aataaatatc gccagatcca cgacgccttc gaaaaaaccg
     6301 ggcgtcactg gctgtacaat gccaccgtcg gtgcgggctt accgatcaac cacaccgtgc
     6361 gcgatctgat cgacagcggc gatactattt tgtcgatcag cgggatcttc tccggcacgc
     6421 tctcctggtt gttcctgcaa ttcgacggta gcgtgccgtt taccgagctg gtggatcagg
     6481 cgtggcagca gggcttaacc gaacctgacc cgcgtgacga tctctctggc aaagacgtga
     6541 tgcgcaagct ggtgattctg gcgcgtgaag caggttacaa catcgaaccg gatcaggtac
     6601 gcgtggagtc gctggtgcct gcccattgcg aaggcggcag catcgaccat ttctttgaaa
     6661 atggcgatga acttaacgag cagatggtgc aacggctgga agcggcccgc gaaatggggc
     6721 tggtgctgcg ctacgtggcg cgtttcgatg ccaatggcaa agcgcgtgtg ggcgtggaag
     6781 cggtgcgtga agaccatccg ctggcatcac tgctgccgtg cgataacgtc tttgccatcg
     6841 aaagccgctg gtatcgcgat aatccgctgg tgatccgtgg gcctggcgct gggcgcgacg
     6901 tcaccgccgg ggcgattcag tcggatatca accggctggc acagttgttg taatttcaga
     6961 aatttaataa tgcccggtac tcatgttttc gggtttatgg tttctaatga aatatattga
     7021 attatcatag gattaggccg gattaagcgt ttacgacgaa tccggcaaga agcaataagt
     7081 acatggttag tttatatttg cagtccggtt tgctttgcat accggatttt ctttttctta
     7141 ccatcctgaa gttttttcat cttccctgat ttttcctcac catcattggt catttttcgg
     7201 ttgacgccct tcggcttttc cttcatcttt acatctggac gtctaaacgg atagatgtgc
     7261 acaacacaac atataactac aagcgattga tgaggtaagg tatgagcttt tttcacgcca
     7321 gccagcggga tgccctgaat cagagcctgg cagaagtcca ggggcagatt aacgtttcgt
     7381 tcgagttttt cccgccgcgt accagtgaaa tggagcagac cctgtggaac tccatcgatc
     7441 gccttagcag cctgaaaccg aagtttgtat cggtgaccta tggcgcgaac tccggcgagc
     7501 gcgaccgtac gcacagcatt attaaaggca ttaaagatcg cactggtctg gaagcggcac
     7561 cgcatcttac ttgcattgat gcgacgcccg acgagttgcg caccattgca cgcgactact
     7621 ggaataacgg tattcgtcat atcgtggcgc tgcgtggcga tctgccgccg ggaagtggta
     7681 agccagaaat gtatgcttct gacctggtga cgctattaaa agaagtggca gatttcgata
     7741 tctccgtggc ggcgtatccg gaagttcacc cggaagcaaa aagcgctcag gcggatttgc
     7801 ttaatctgaa acgcaaagtc gatgccggag ccaaccgcgc gattactcag ttcttcttcg
     7861 atgtcgaaag ctacctgcgt tttcgtgacc gctgtgtatc ggcgggcatt gatgtggaaa
     7921 ttattccggg aattttgccg gtatctaact ttaaacaggc gaagaaattt gccgatatga
     7981 ccaacgtgcg tattccggcg tggatggcgc aaatgttcga cggcctggat gatgatgccg
     8041 aaacccgcaa actggtgggc gcgaatattg ccatggatat ggtgaagatt ttaagccgtg
     8101 aaggagtgaa agatttccac ttctatacgc ttaaccgcgc tgaaatgagt tacgcgattt
     8161 gccatacgct gggtgttcga cctggtttat aaatagtgtg gcttttgtga aaatcacaca
     8221 gtgatcacaa attttaaaca gagcacaaaa tgctgcctcg aaatgagggc gggaaaataa
     8281 ggttgtcagc cttgttttct ccctcattac ttgaaggata tgaagctaaa accctttttt
     8341 ataaagcatt tgtccgaatt cggacataat caaaaaagct taattacgat caatttgacc
     8401 tacatctctt taaccaacaa tatgtaagat ctcaactatc gcatccgtgg attaattcaa
     8461 ttataacttc tctctaacgc tgtgtatcgt aacggtaaca ctgtagaggg gagcacattg
     8521 atgagcacgt cagacgatat ccataacacg acagccactg gcaaatgccc gttccatcag
     8581 ggcggtcacg accagagcgc gggggcgggc acaaccactc gcgactggtg gccaaatcaa
     8641 ctccgtgttg acctgttaaa ccaacattct aatcgttcta acccgctggg tgaggacttt
     8701 gactaccgca aagaattcag caaattagat tactacggcc tgaaaaaaga tctgaaagcc
     8761 ctgctgacag aatctcagcc gtggtggcca gccgactggg gcagctacgc cggtctgttt
     8821 attcgtatgg cctggcacgg cgcaggtact taccgttcaa tcgatggacg cggcggcgcg
     8881 ggtcgtggtc agcaacgttt tgcaccgctg aactcctggc cggataacgt aagcctcgat
     8941 aaagcgcgtc gcctgttgtg gccaatcaaa cagaaatatg gtcagaaaat ctcctgggcc
     9001 gacctgttta tcctcgcagg taacgtggcg ctggaaaact ccggcttccg taccttcggt
     9061 tttggtgccg gtcgtgaaga cgtctgggaa ccggatctgg atgttaactg gggtgatgaa
     9121 aaagcctggc tgactcaccg tcatccggaa gcgctggcga aagcaccgct gggtgcaacc
     9181 gagatgggcc tgatttacgt taacccggaa ggcccggatc acagcggcga accgctttct
     9241 gcggcagcag ctatccgcgc gaccttcggc aacatgggca tgaacgacga agaaaccgtg
     9301 gcgctgattg cgggtggtca tacgctgggt aaaacccacg gtgccggtcc gacatcaaac
     9361 gtaggtcctg atccagaagc tgcaccgatt gaagaacaag gtttaggctg ggcgagcact
     9421 tacggcagcg gcgttggcgc agatgccatt acctctggtc tggaagttgt ctggacccag
     9481 acgccgaccc agtggagcaa ctatttcttc gaaaacctgt tcaaatatga gtgggtacag
     9541 acccgcagtc cggctggcgc aatccagttc gaagcggtag acgcaccgga aattatcccg
     9601 gatccattcg atccgtcgaa gaaacgtaaa ccgacgatgc tggtgaccga cctgacgctg
     9661 cgttttgatc ctgagttcga gaagatctct cgtcgtttcc tcaacgatcc gcaggcgttc
     9721 aacgaagcct ttgcccgtgc ctggttcaaa ctgacgcaca gggatatggg gccgaaatct
     9781 cgctacatcg ggccggaagt gccgaaagaa gatctgatct ggcaagatcc gctgccgcag
     9841 ccgatctaca acccgaccga gcaggacatt atcgatctga aattcgcgat tgcggattct
     9901 ggtctgtctg ttagtgagcg ggtatcggtg gcctgggcat ctgcttctac cttccgtggt
     9961 ggcgacaaac gcggtggtgc caacggtgcg cgtctggcat taatgccgca gcgcgactgg
    10021 gatgtgaacg ccgcagccgt tcgtgctctg cctgttctgg agaaaatcca gaaagagtct
    10081 ggtaaagcct cgctggcgga tatcatcgtg ctggctggtg tggttggtgt tgagaaagcc
    10141 gcaagcgccg caggtttgag cattcatgta ccgtttgcgc cgggccgcgt tgatgcgcgt
    10201 caggatcaga ctgacattga gatgttcgag ctgctggagc caattgctga cggtttccgt
    10261 aactatcgcg ctcgtctgga tgtttccacc accgagtcac tgctgatcga caaagcccag
    10321 caactgaccc tgaccgcgcc ggaaatgacc gcgctggtgg gcgggatgcg cgtactgggt
    10381 gccaacttcg atggcagcaa aaacggcgtc ttcactgacc gcgttggcgt attgagcaat
    10441 gacttcttcg tgaacttgct ggatatgcgt tacgagtgga aagcgaccga cgaatcgaaa
    10501 gagctgtttg aaggccgtga ccgcgaaacc ggcgaagtga aatacactgc cagccgtgcg
    10561 gatctggtat ttggttctaa ctctgtcctg cgtgcggtgg ctgaagttta cgccagcagc
    10621 gatgctcacg agaagtttgt taaagacttc gtggcggcat gggtgaaagt gatgaacctc
    10681 gaccgtttcg acctgctgta atctgacctt cttcagcgac tgcctttcag gcagtcgctg
    10741 aagtttcttt accggcgtat agtgtccaca ggaaaactac acactggatc tctcatgtct
    10801 gccgcaggaa agagcaaccc actggcaatc agtggcctgg ttgtgctcac acttatctgg
    10861 agttatagct ggattttcat gaagcaagtc accagctaca tcggtgcctt cgactttacc
    10921 gccttacgct gcattttcgg cgctctcgtt ttattcatcg tccttttatt acgtggtcgc
    10981 ggaatgcgcc cgacaccgtt taaatacacc ttagccattg ccctgttaca aacctgcggg
    11041 atggttggcc tggcgcagtg ggcgctggtc agcggcggag cggggaaggt ggcgatccta
    11101 agctatacca tgccgttctg ggtggtgatt ttcgccgcgt tgtttctcgg tgaacgcctg
    11161 cgacgtgggc aatatttcgc gatactgatt gccgctttcg gcttattttt ggtgttgcag
    11221 ccgtggcaac tcgatttctc ttcgatgaaa agtgccatgc tggcaatcct ctccggcgtc
    11281 agttgggggg cgagcgcgat tgttgctaaa cgtctgtatg cccgtcatcc gcgcgtggat
    11341 ttattgtcgt taacatcctg gcagatgctc tatgcggcgc tggtgatgag tgtggtcgct
    11401 ttactggtgc cgcaacgtga aattgactgg cagcccaccg tgttctgggc gctggcctac
    11461 agtgcgattc tggcgacggc actggcgtgg agcttatggt tgtttgtatt gaaaaacttg
    11521 cctgccagta ttgccagctt aagcacactg gccgttcccg tttgcggcgt actcttttcc
    11581 tggtggctgc tcggcgagaa tccgggggcc gttgaaggta gcggtattgt gctgattgtg
    11641 atggcactgg cgctggtgag ccgtaagaaa aaagaagccg ttagtgtaaa aaggatctga
    11701 attttttctt catgtggggc gatcgcttat ttaacaaaat aacgataatg ccccaccatg
    11761 cgccagctaa acagcacatc ttcttcctgc gcacctgcgc caatgttatg tatcaccagc
    11821 ggcgtaccgt cgcgggcgaa gccatctgaa accaccccaa tatgtgccag cccgttatct
    11881 aatcgccagg agacaatatc gcccgcttga tagtcactgg ggttcttgct gatggggcgt
    11941 gttttatcgt ggtgggtaaa ccaggtttcc agattaggca cccgacggtg atcgatgttg
    12001 ctgtccgggc gctttaactg ccatttttgc gggtactcgg caaaattctt cgccatatct
    12061 tcgtgcacca gtttctgcaa atcgaccttc tggctgcgca atgcacggat caccacatcg
    12121 gaacataccc cgcgtttttg cgggacatca ccgccaggat aggtaagctg catatacgcc
    12181 ggatcgtaaa atagcgtgct gccgatttgc tgtctggcac cgtctgcgat ggcaaggttg
    12241 gtattggcct ggatttgtac cacggttggt ggaacggcgg gagattttaa ggagtggctg
    12301 gtaaatgccg ttagcaggct gagcagcgcc agtgaagctt tcatctggtt cgtccgtgaa
    12361 aataagagtc acagattcga ccttccggga taacgattgt tctgtagaga aaatgtccag
    12421 aagcggataa aaaactcctg aacaacgtgg gagtttcaga ttactggcac agagtgccgg
    12481 atgcggcgtg aacgccttat ctgtcctaaa aaaaaagaaa attcaatata ttgcagagat
    12541 tgcgtaggcc tgataagcgt agcgcatcag gcaattttgc gttcaaactc ccggacaagc
    12601 cgggagtttg gagtaggtta ttcccactct tgcaggaaac gctgaccgta ctggtcggct
    12661 accagcagag cggcgtaaac ctgatctggc gtcgcgccgc caggcatgtt gtggatggtt
    12721 tcacattctg cacatgccgc ttcagccaca attcgcattt tcgccgggac atcttcttta
    12781 atatccagtt gagcgagagt tattggcaaa cctaccgcat ggctaagtgc agctacggtt
    12841 tcgatttcct caaccggtgc gttttccaga accagctgcg tcagcgtacc aaatgccact
    12901 ttttcaccgt gataatagtg atgcgcgtcc gggatagcgg tcaggccgtt atgcactgcg
    12961 tgcgccgcag ccagaccacc actttcaaaa ccaacaccgc tcaaataggt gttcgcttca
    13021 atcacgcgct ccagcgccgg agtcactaca tgctgttcgg cagcaagcat cgctttttcg
    13081 ccttcttcca gcagggtgtt gtagcacagt tcagccagtg ccagcgcagc ctgggtgcac
    13141 ttgccgcccg ccatggtggt cgcgccgcta cgagagcagg cacgcgcttc aaaccaggtt
    13201 gccagcgcat cgccgatacc cgccgctaac agacgtgcag gtgcgccagc gacgattttg
    13261 gtgtcgacaa tgaccatatt cgggttattt ggcaacagca gatagcggtc aaactcaccc
    13321 tcatcggtgt agataacaga caatgcgctg cacggtgcat cggtagaggc gatagtcggt
    13381 gcgatcgcta ccggaacacc catgaaatgt gccagtgctt tggctgtatc gagggttttt
    13441 ccgccaccga taccgagaat tgcgccacac tgcgcagtct ccgcgatgcc acgcagacgg
    13501 tcgatctcat tttgcgaaca tccaccgcca aacggcgcaa tttctactac cagtccagca
    13561 tctttaaagc ttttctcgac agtggattga gcaaaaccta aaacaaattt gtcacccacc
    13621 actaaccagc gttctgccag cggcttcagg tattcgccca gacgattaat cacatcagcg
    13681 ccctggatgt atttacccgg tgattgaata atgcggtcca taattgctcc tttagagatg
    13741 agtagtgcca aatgcggcat tccagtcgtg ttcgaacttc tctatagctg actctaccgc
    13801 aggggtgttg agcatttgtt gcgctacatc taagggcagg gtgatggatt cacatcctgc
    13861 cagtaaacag tccagcgcct gacgcggcgt tttaaagctg gctgccagca ccatgctttc
    13921 tggcgcgtgc atttctaaca gcgcttgcag ctcctgaacc gtacgaatgc cgtctccgcc
    13981 ctgggcatct acgcggttaa catacggagc aacgtatttt gcccctgcca gcgcggccag
    14041 taacccttgt gcagcactgt acactgcggt gccaagcgta gtaataccct cttttttcag
    14101 tattttaatt gctgccagac cttcggaagt caccgggatt ttcaccacaa tacccggaat
    14161 agcgtcgcgc aggcgcttcg cttcttccac catcccctgc gcgtcgcggc tcatggtctg
    14221 agcaaacaga atgccctcat caccaatcgc tttttgcaga cgcggcagca cttcccatat
    14281 ggactccttg ctggcagcga taatgctcgg gttagttgtc accccggcaa tggggaatat
    14341 gcgtgccaga cgttcgactt ctgcgacgtt agcggtgtcc agatacagtt ccatgatgtt
    14401 tccttttaca gttccagttc atgttgcagc aggctggcga tagcgtcggc agaggcggcg
    14461 ttaaccagcg cgttacggaa ttcttcgtgc atgatgcggc gagcgaggcg cgagaaaatg
    14521 cgcatgtgct gatcgcccgc agcgtgtttg ttcagggtta acatgatgat gaattgcgct
    14581 tcatcatcac cccagcgcac cggcgcttgc agacgcgcaa cgctgatggt ggattgctca
    14641 atgtgttctg atttgctgtg tggaatggca aaactaaagc ccagaccggt agagaaaacg
    14701 gcctcgcgcg cccacaagtc ggcttccagt ttacgcggat agcggcagcg ccctgccagc
    14761 agcaggttat cggtcatgcc tttgagcact tcttctttgc tgcgccagtc gctttccagt
    14821 gtgatgcact cggcggtgac cagcggtgcg tcttgttggg tcatgcggaa ttgcgccagc
    14881 aggtgctcca cttccagcga agtacggcag gccattgcct ggttgagcaa cttgcggcac
    14941 tcacggctat caagttgcgc catccgcgct ttcgccgccg gaattgatgg tgcgctcatg
    15001 ctgagttcat ccagccctaa gccgaccagc aacggcagca cggaaccttt cgctcccagc
    15061 tcaccgcaca gaccaatcca tttaccctgg cgatgcaccg cctgcacggc gtaatcgagc
    15121 gcccgcaaga atgccggatt caggctgttg tagtgacgag taaccttagc gttatcgcga
    15181 tccactgcca gcagatattg cgtcaggtca ttactaccaa tactaaagaa atcaatctct
    15241 tcgcagcatt gatcgatgat gaacatcacc gacggcactt ccagcataat gccgagctgg
    15301 atcttctcat caaacggaat gtgttcgtta cgtagttgct gtttggcttc tgccagtttt
    15361 tctttcaccc ataagatctc ttccattgag gagatcatcg ggatcatgat tttcaggctg
    15421 ccgtgagcgg aggcgcgcag gatcgagcgt agttgtgtgg taaataacga cgcgtattct
    15481 tcataaatac gcacggcgcg ataaccgagg aacgggtttg cctctgcggg aatgtttaga
    15541 taatcaacgg gtttgtcgcc gccaatgtcc atagtgcgca caataatgct gcgtccgttg
    15601 gcagactcca gcgcctgaca aaaaatgttg tacagctcgc tttcgcccgg tgcactggtg
    15661 cgatccatat agagcatttc agtgcggaac aaaccaacgc cttccgcacc attgccgaat
    15721 gcggcctgcg cttccacgga gtgagcgatg ttagcggcaa tttcaatgcg gataccgtca
    15781 gcggtacggg cttgttgggt cagccagaca cgctgttgct cacgcagggc gtcctgtacg
    15841 cgggcttctt gctgataata acgagctacg gcttctcctg gctcaaccac aatcgccccg
    15901 gcgttgccgt cgatataaat cgtttgatgc tgccacggag tgagggcatc aatatccaca
    15961 ccaaccagcg tcggaatgtt gaacgaacgg gcaaggatca ccgtatgtga ggtggtgccg
    16021 ccacttttca gcaacagacc tttgaggtga tttttatcca gttcgaggaa ctggctgggg
    16081 gtcagttcat cagccataca aatggcggac tgcgtcagtt tgcccggtgc cgggaagcgt
    16141 tgctcaccgt agatttgctg gagtaactgg aagcagacgt cgcgtacgtc cagggcacgt
    16201 tcttgcaggt agctgctgct ggaacgggcg aactcttcgc aaaagtgatt cgcactggcg
    16261 acaatcgctt cggcgcagct taatccctcg ctgacgcctg ccagtaagtg ctgacgtaaa
    16321 gatgtatcgc cagccaggga tcggtgagct tccagtatcg cgctggtagc accgtcgcta
    16381 tccagcagac gaaactcaat gtttttcagt accagcgtca ggccgttttc cagtgtggat
    16441 tgctcggcgt caacgtcttt ggctgcggga agattactca gcgcattgag atctaaagaa
    16501 gagatcggcg tcagaatgcc gcctgcacta ccgctgcaca cggtgcgggc gcggataatt
    16561 tgcggattca gattggtcag tgaaaccggc agtggttcca gttcgtcaga tttaacttcc
    16621 gccagcggcg catcgcagtg ggggaattca tcgcgcagcc attggcttaa acgctggtgc
    16681 gcttcctgtt cgtcggcccc ggaaatcagt aactggcagt tatcgcccgc cagcgtatcg
    16741 gtgccaatca gcgcaagggc gcttttggcg ttgcccttgc ggtcagtgtg caggttatgc
    16801 cactcaattt gtgatgaaaa agtattacac agcgtttcaa cgtggcttgc cggacgcgca
    16861 tgtacgccgt taggtagctc acaaataaat tccacaatca gggccattgc ctctctcccg
    16921 gtacggtttt ctgtttacag gataagagcg cacggcaacg gcctgccatg tgacaaatct
    16981 gccaaaagct ggacaaatgt aatgtaaccg tcaatttgcg acgcgtctca caagacgccg
    17041 ttttgcggca tgcttccggt ttatcgcaag ttatgaggcg gatcgcattt ttgtactgat
    17101 attacaaaag tccagtaaat ggccttttta tccactgttt gctccgcctg cgattgccta
    17161 ttgtttcctc catcaaaaat atggacatgg gctttcgcaa gcccgggagc agcttatgaa
    17221 tgagttggtg cagatcctga aaaatacccg tcagcattta atgacgggcg tttcacacat
    17281 gattcccttc gtggtatcgg gcggtatttt gctggcggtt tccgtcatgt tgtatggcaa
    17341 aggcgcagtg ccggatgccg tagccgatcc aaatctgaaa aaactgtttg atatcggcgt
    17401 tgcgggcttg acgctgatgg tgcctttcct cgccgcttac attggttatt ccattgcaga
    17461 acgttctgcg ctggctccat gcgctatcgg tacctgggtt ggtaacagct ttggtgcggg
    17521 cttctttggt gcactgatcg ccgggattat cggcggcatc gtggtgcatt acctgaagaa
    17581 aattccggtg cataaagttc tgcgctcggt gatgcctatc ttcattattc ctatcgtcgg
    17641 cacactgatt accgcaggca tcatgatgtg gggcttgggc gagcctgtag gggcgttgac
    17701 caacagcctg actcagtggc ttcaggggat gcagcagggc agcattgtta tgctggcggt
    17761 gatcatgggt ctgatgctgg cgttcgatat gggcggtccg gttaacaaag tggcctatgc
    17821 cttcatgctg atttgcgttg ctcagggtgt ttataccgtg gtggccatcg ctgccgtggg
    17881 tatttgtatc ccgccgctgg ggatggggct ggcgacgctg attggtcgta aaaatttctc
    17941 cgcagaagag cgcgaaaccg gcaaagcggc actggtgatg ggctgtgttg gcgttactgg
    18001 aggggcgatt cctttcgccg ctgccgatcc gctgcgtgtc attccttcca tcatggtcgg
    18061 ttcagtttgt ggtgcagtaa ctgcggcgct ggtcggtgcg cagtgctatg caggctgggg
    18121 tggtctgatt gtgctgccgg tggttgaagg caagctgggt tatatcgcag cagtggctgt
    18181 cggagcagtg gtgacggctg tttgtgtgaa cgtgctgaaa agtctggcgc gtaaaaatgg
    18241 gtcttcgact gatgaaaaag aagacgacct ggatttggat tttgaaatta attaattgag
    18301 gaacgagcca tgacgaaaat tattgcagta accgcatgtc cttcaggtgt tgcccatacc
    18361 tatatggcag cagaggcgct ggaaagtgcc gcgaaagcga aaggctggga agtgaaagta
    18421 gaaacctagg ggtcaattgg tctggaaaat gaactgactg cggaagatgt ggcgagcgct
    18481 gatatggtga ttctgaccaa agatatcggc atcaagtttg aagagcgttt tgctggcaag
    18541 accatcgtgc gcgtcaacat cagcgatgca gtaaaacgtg ctgacgccat tatgagcaaa
    18601 attgaagctc acctggcgca aaccgcttaa gttcttctcc cgctcgcaag ggcgggttcg
    18661 ctttcccaca ggagttcctc atgacgaatc gtatctctcg cctcaaaact gcactgtttg
    18721 ccaatacccg tgaaatctcg ctggagcggg cgctgcttta taccgccagc catcggcaaa
    18781 ccgaaggcga accggtgata ttgcgccggg cgaaagcaac agcgtatatc cttgaacatg
    18841 ttgaaatttc gattcgtgat gaagaactga ttgccggtaa ccgcaccgta aaaccgcgcg
    18901 ccgggattat gtcgccggaa atggaccctt actggctgct gaaagagctg gatcaattcc
    18961 cgacgcgtcc gcaggaccgc tttgctatca gcgaagaaga taaacgtatc taccgtgaag
    19021 agttgttccc gtactgggaa aaacgttcga tgaaagattt catcaacggg cagatgacag
    19081 atgaagtaaa agccgcgacc aacacgcaga ttttcagcat caaccagacg gataaaggcc
    19141 aggggcacat tattattgat tacccacgcc tgttgaatca cggtctgggt gagctggtgg
    19201 cacagatgca gcaacattgt cagcaacagc cggagaatca cttttatcag gccgcgttgt
    19261 tactgctgga agcctcgcag aaacacattt tgcgttacgc cgaactggcg gaaacgatgg
    19321 cggcaaactg cacagatgca cagcgtcgcg aagaactgct gactattgcg gagatctctc
    19381 gccataacgc cgaacataag ccgcagacgt tctggcaggc gtgccagtta ttctggtaca
    19441 tgaacatcat tctgcaatac gaatccaacg ccagttcgct gtctttaggg cgcttcgacc
    19501 agtatatgtt gccgttctat caggcatctt taacccaggg cgaagatccg gcgttcctga
    19561 aagaactgct cgaatcttta tgggtgaaat gcaacgacat cgtgctgttg cgctccacca
    19621 gcagcgcgcg ttatttcgca ggtttcccga ccggctatac cgcactgctc ggcgggttaa
    19681 ccgagaacgg acgtagcgcg gtgaacgtgc tttcgttcct ttgccttgac gcctatcaaa
    19741 gcgtgcaatt accgcaaccg aacctcggcg tgcgcactaa cgccttgatc gacacgccgt
    19801 tcctgatgaa aaccgccgaa accattcgcc tcggcaccgg tattccgcaa atctttaacg
    19861 atgaagtggt ggtaccagcg ttcctcaacc gtggcgtttc gctggaagat gcgcgcgact
    19921 attccgtagt gggctgtgtg gaattatcta ttcccggcag aacctacggc ttgcatgaca
    19981 tcgcgatgtt taatctgctg aaagtgatgg aaatctgcct gcatgaaaat gaaggcaatg
    20041 ctgcgctgac ttatgaaggt ttactggagc agatccgcgc caagatcagc cactacatca
    20101 ccctgatggt tgagggcagc aatatttgtg atatcggcca tcgcgactgg gcacctgtac
    20161 cgctgctctc gtcttttatc agcgattgtc tggaaaaagg tcgcgatatt accgatggcg
    20221 gcgcgcgtta taacttctcc ggcgtacagg ggatcggtat cgccaacctg agcgattctc
    20281 tccatgcgtt gaaagggatg gtttttgagc aacagcgttt aagttttgac gaattgctgt
    20341 cggtattaaa agccaacttt gcaacgccag aaggcgaaaa agtccgtgct cgcttaatta
    20401 accgcttcga gaaatacggt aacgatatcg acgaggtgga taacatcagc gccgaactgt
    20461 tacgccacta ctgcaaagaa gtggaaaaat accagaaccc gcgcgcggtt acttcacgcc
    20521 gggatcgtat accgtttctg ctcacgtccc gttgggatcg gtggttggcg cgacgccaga
    20581 cggtcgtttt gccggggaac aactggcaga cggcggcttg tcacctatgc tgggccagga
    20641 cgcacaaggg ccaacagcgg tactgaagtc agtcagtaag ctcgataata cgctgctgtc
    20701 taacggtacg ttgctgaacg tgaaattcac tccggcgacc ctggaaggtg aagcgggatt
    20761 acgcaaactg gccgacttct tacgggcgtt tacccagctt aagttgcagc atattcagtt
    20821 taacgtggtg aacgccgaca cgttgcggga agcgcaacag cgcccacaag attatgccgg
    20881 gctggtggtg cgcgttgccg gatacagcgc cttctttgtc gaactgtcga aggagatcca
    20941 ggatgacatc atccgccgga cagcgcatca gctgtaacgt tgtggaaacg cgccgcgatg
    21001 atgtggcgcg cattttcaac attcagcgtt attcactgaa tgacggcgag ggcattcgta
    21061 cggtggtctt ttttaaaggc tgtccgcatc tttgcccgtg gtgtgctaat ccggagtcga
    21121 tctccggcaa aatccagacg gtacgcagag aggcgaaatg tctgcactgt gcgaaatgtt
    21181 tgcgtgatgc ggatgaatgc ccctccgggg cgtttgaacg gattggtcgc gatatcagcc
    21241 ttgacgctct ggaacgggaa gtgatgaaag atgacatttt ctttcgcacg tccggcggcg
    21301 gcgtcacgct ttctggcggc gaagtgttaa tgcaggcgga gtttgctacc cgttttttac
    21361 agcgactgcg gctgtggggt gtgtcatgcg ccattgaaac tgccggagac gcgccagcaa
    21421 gcaagctgtt accgctggcg aaattgtgcg atgaagtgct gttcgattta aaaattatgg
    21481 acgcgactca ggcgcgggat gtggtgaaga tgaacctgcc acgcgtgctg gagaatctgc
    21541 gtttgctggt gagtgagggt gtcaacgtga tcccgcgttt accgttgatc cctggtttca
    21601 cgctcagcag ggagaatatg cagcaggcgc tggatgtact gatcccgctg aaaatcaggc
    21661 agatccatct gttaccgttt catcagtacg gcgaaccgaa ataccgcctg ctggggaaaa
    21721 catggtcgat gaaagaggtg cctgcgccgt cgtcagccga tgtgacaacg atgcgcgaaa
    21781 tggcagaacg ggccggattt caggttaccg tgggaggtta aaatggcata cctggtggca
    21841 gtaaccgcct gcgtaagcgg cgtggcgcat acttatatgg cggcggaacg gctggaaaag
    21901 ttgtgccagt tagagaagtg gggcgtcagc attgaaactc agggcgcgct gggaacggag
    21961 aatcgtttag cggacgagga tatccgtcgg gcggatgttg ctctgttgat tacggatatc
    22021 gagcttgccg gtgccgagcg atttgaacat tgccgctatg tgcaatgcag catctacgca
    22081 ttcctgcgtg agccgcagcg ggtaatgagc gcggtgcgca aagtgctttc tgcgccgcag
    22141 caaacccatc ttattctgga gtagtcggtt tttctgtcag ttggctgtgg tactgccggc
    22201 gatattccga cggcgagcgt tcggtgtttt tacgaaacag acggcagaag tagttgctgt
    22261 cgacaaagcc gcaggcgtgc gccacttctt ttaccttcag gtcatagcct ttcaataacg
    22321 tcttagcgtg ctccagtcgc gtgtgattca ggtattcgtt aaaaccaatg gcccccgttt
    22381 tttgaaacag gtgcgagaga tagtttggtg aaatatagaa tgcctgtgcg acagattcgc
    22441 gggtaagcgc ggaggcatag cgttcgtcga tataatcgcg aatagcttca aatagtgcct
    22501 ggctgcgtga ggcggtctgg atttggctgc cgagcaaatc gcggcagtgg ctaagcaggc
    22561 tggctacgat aagccttgcc gtttgctgct cctgcggctg catttgcatt tcattgagcg
    22621 tttgtagcag aaaagaacct atacgtgggc cgcgtcgggc gacgtgttgc ttcgccagat
    22681 tttgatattg tttgccatcc cattgcacga cgctgaagcc gagttgctgt ttgccaaaca
    22741 gcacgctaaa ggtggtagcg ggtgttttcc attgtgggaa attccagcca ccagcggcaa
    22801 cgtacagcac atcgccggga actaacgttg ctccagcgcc ggtatcaaca aactcacctt
    22861 ccagcacaat ttccagccgt ggaaaatcga cctgatacgc aagatcgggc accgggccgt
    22921 tactgctggc aaagtaaatc tggcgcaggg acagcgggcc gttgatcagg cgggagagca
    22981 gatagctgac gccgtgatac atgttattcc cttaggtatg tagacgggaa aacagtaact
    23041 ggcattatta aaaaaggcca cctgaaaagt gtggcctgag gggagttcga tgtaaacatt
    23101 gcggcagttg tattgccgga tgtggcgtaa acgccttatc cagcctaccg ccgcgatctg
    23161 taggccggat aagacgcgtc aagcgtcgca tccggcaaat agcgcctggc tgataattac
    23221 tgattaccta cctggtcgcc atacggcaga gtgtcgtaat cgatcagcgc gtttttcttg
    23281 tacgggttac caatccagcg ggtagtttct ttgaactgcg gattcaccac tgaacgggtt
    23341 ggatcgtaac cgtcgtaaga taagcccgcc aaatctgacc aggtgtggat cagttccgcc
    23401 aggctgtatt tacgatcgac atcctgtgag aaatcacggg gatgagtcgc ttgccatttt
    23461 tccgacgtcc acagcaggaa cggaatggtg tacatgtggc gcgtcgggtt gtcttcgtta
    23521 cgcccctgag ttttgtgcgg cggcgtgtcg taaacctctt caccgtggtc agagaagtaa
    23581 accaggaatc cgttcgggtc tgccgcttta aagtctttaa tcaggctggc aaccacatga
    23641 tcgttataca ggttagcgtt gtcataatcg ttatatgatt ccagctcttc tgcgcttaat
    23701 cctggcggaa catgatcggt attgccatca aacttgccct gattttccgg gtagcggtat
    23761 ttgtatttga tatgcgtacc cagcagatga acgatgatca gtttcttcgg cgcagggtca
    23821 ttcagcacat cctggaacgg cttcagcacg ttggtgtcgt attcacgcgc actctgcgta
    23881 cgttgctggt tcatgtagta ctgcttgtcg gtctggcgcg aaaataccgt cagcatggta
    23941 ttgcgggcgg tcatcgtctg ctggttggtg atccagaagg ttttataacc cgcctgtttc
    24001 atcatgttca tcagcgacgg ctgcgtcaga tacagatccg ggttcttttc attggcaaag
    24061 gtcagcgcct gttgcaggat ttcaatggtg tacggacgag acgtaactac gttattaaac
    24121 acggtcagat tcggatcggt tttatgcagc gcatccagct ccggcgtggt ttcacgcgga
    24181 taaccgtaca gactcatgcg tccgcgctgg gtcgactcgc caatcaccag cactaaagtg
    24241 cgcggttcgt tacccgattc atctttgaaa ttagccagtg gcggcaaggc attattttca
    24301 ttcagtaact ttgttagcga gtttagttgc tgacgatact gataatagcc ggtcaggaat
    24361 tgccacggtg cggcaggctc catacgcgag gccaggttat ccaacgtttt ctcaaacggc
    24421 ttgtttttga taaacgtatt catggcgatc ggatgcagaa tcaagccata aagcagggca
    24481 aaagagacaa cataacgcca cggctttgga atatagaccg ggcgcaggcg tgtccacagc
    24541 agaactgcca ccgccgtata ggccagcgcg ataagcacaa ttttcaggct gaaatactgg
    24601 cttaaatact cgctggcttc gttggtgttg gtttcgaaca tcacaaacag aacgctctgc
    24661 gagaactcct gcccgtagat gacgtagtag catagcgccg ccagagaggc cgcccatagc
    24721 accacgccga ttactgcggc aataatttta atccgcttcg gaaagaggaa taccgggatc
    24781 aaccacagcg agctgaataa cagcgagtcg cgaatgccgt tggtgccact ataaccactg
    24841 atgtaaataa tggcctgtag cagagtagag aaaaaccaaa agtagagcag tgcccaaccc
    24901 agggctttcc agctaaaaag gggtttagcc tggacttctg tggaatgcat agtaagaacc
    24961 tgtcttgaaa aaaatatcgc cgaatgtaac gacaattcct taaggatatc tgaaggtata
    25021 ttcagaattt gaataaaatg cagacagaaa tatattgaaa acgagggtgt tagaacagaa
    25081 gtatttcaga aaaccctcgc gcaaaagcac gagggtttgc agaagaggaa gattagccgg
    25141 tattacgcat acctgccgca atcccggcaa tagtgaccat taacgcttgt tcgacgcgag
    25201 gatccggttc ctggccttct ttttctgcct ggcgggagcg gtgcagcaac tcggcctgca
    25261 atacgttcag cgggtcggtg taaatattcc gtagctgaat agactctgca atccacggca
    25321 gatcggccat cagatgggaa tcgttggcaa tcgccagcac cactttgatg tcttcttctt
    25381 gcaggttgcg taactcttta cctaacggcc acagtgcttt gtctaccaga cgttgatcat
    25441 agtattccgc caaccacagg tctgctttgg cgaagaccat ctccagcatg ccgagacgcg
    25501 tcgagaagaa tggccaatcg cggcacatgg cttccagctc gctctgtttg ccatcttcga
    25561 ccactttttg cagcgccgta cctgcaccca gccaggcggg gagcatcagg cggttttgcg
    25621 tccaggcaaa aatccacggt atggcgcgca gcgactcgac gccgccggtt gggcgacgtt
    25681 tcgccggacg tgaacccaac ggcagtttgc ccaattcttg ttccggcgta gcggagcgga
    25741 agtaaggcac aaaatctttg ttttcacgta cgtagccacg gtagagatcg caggagatga
    25801 ctgacagttc atccataatg cgacgccagc tctctttcgg ctcaggtggt ggcagcaggt
    25861 tggcttccag aatcgccccg gtgtaaagcg acaggctgct gacggtgatt tctggcagac
    25921 catatttaaa gcggatcatc tcgccctgtt cagtcacgcg caggccacct ttcaggcttc
    25981 ctggcggttg tgacagcagc gccgcatggg caggtgcgcc gccgcgacca atggaaccgc
    26041 cgcgaccgtg gaacaacgtc agctcaatac ccgctttttc gcaggttttg attaatgcat
    26101 cctgtgcctg atattgcgcc caggaagctg ccatcacgcc cgcatctttt gctgagtcgg
    26161 aatagccaat catcaccatc tgtttgccct gaatcaggcc gcgataccag tcgatattga
    26221 gcagctgggt catgacatcg ttggcgttgt tcaggtcatc gagggtttca aacagcggag
    26281 caaccggcat cgcaaacccg atacccgctt ctttcagcag caggtggaca gccagtacgt
    26341 cggacggcgt tttcgccatc gagatcacgt aggcggcaat ggagccttgc ggcgcttcgg
    26401 caatcacctg gcaggtatcg agcacttcgc gcgtttcggc gcttggttgc cagttgcgcg
    26461 gcagaagcgg acgtttggag ttcagttcgc ggatcaggaa cgcctgtttg tcggcctctg
    26521 accagctttc gtagtcgccg ataccgaggt agcgggtcag ctcgcccagc gcttcggtat
    26581 ggcgggtgct ctcctgacgg atatcaatac ggaccagcgg tacgccgaaa catttcacgc
    26641 ggcgcagggt gtcgagcaga tcgccgttgg cgataatacc catgccacac gcctgaagtg
    26701 actggtagca agcgtagagc ggttcccaca gttcttcgtt ttgtgtcagc agcccttctg
    26761 gttttggcag ttcttcgcct ttcaggcgcg cttccagcca tgcctgtgtc gccatcaggc
    26821 gagaacgcag gtttttcatc agatagcgat acggttctgc ggcaccttct tcgccaacca
    26881 gcgccagcag ttcaggggtc gcttcaacca tcgacagttc agaaaccaga acctgaatat
    26941 ctttcaggaa cagatcggtg gctttccagc gactgagtag caggacgtgg cgggtgatat
    27001 cggcagtgac gttcgggttg ccgtcgcggt caccgcccat ccacgaagta aaacggaccg
    27061 gaacaaattc gacgggcagt ttgtagccga ggttctcttc cagttgttcg ttcagttcgc
    27121 gcaagtaatt cggtacgcct tgccacaggc tgttttctac tacggcaaag ccccatttgg
    27181 cttcatctac cgggcttgga cgcagcttac ggatttcatc agtatgccat gactgggcga
    27241 ttaactggcg caggcgacgc atcagctggt tgtgttcgta gtcagcgata tctttgttat
    27301 cgagctgttt taagcaggcg ttcacttcca ccattttgtg gatcagtgta cgacgggtaa
    27361 tttcggttgg gtgagccgtg aggaccagtt ccagcgacag cgattccact gcttttttga
    27421 tggtgtcttc gctcagttcc ggctggtttt tcagtttgcg cagggtgcgg gcgatcactt
    27481 ccgggttgct ggcagcttcg cctttcggcg aaatgctgtg gtattgctcg gcggtgttgg
    27541 ccaggttcag gaactgacta aacgcacgcg caacgggcag cagctcgtcg ttcgacaaat
    27601 tttgtaaggt ggtgagcaac tcctggcggt tagcatcatt gccagcgcgt gaagatttcg
    27661 acaacttacg gatagtttct acgcgttcaa gaatgtgttc tcccaacgca tccttgatgg
    27721 tttctcccag cactttgccg agcatactga cattactacg caatgcggaa tattgttcgt
    27781 tcatattacc ccagacaccc catcttatcg tttgatagcc ctgtatcctt cacgtcgcat
    27841 tggcgcgaat atgctcgggc tttgcttttc gtcgtctttt ataaagccac gtaaaagcgg
    27901 tgacgtcaaa tgctgcgaaa tcgcttcagc aaacgaataa atagcaggaa tttacgccat
    27961 taaattcacg acgctttaaa taagcgtaac ttatggaaat gttaaaaaat cgccatacat
    28021 aacaccaaag gtgtaggtcg gataagatgc gcaagcatcg catccgacat tattgcggca
    28081 ccggagtttg gcaacggtgc cggatgcggc gcgagcgcct tatccggcct acgttttaat
    28141 gccagcaaaa atggtgaatt acctggataa tcaactcacg ggtgggttta ataaaccgcg
    28201 tctccagata ttcatcaggc tgatgagctt gattgatgga acccggaccg agcaccagcg
    28261 tcgggcataa cgtttgaata aacggcgctt cggtacagta attcaccact tcggtttttg
    28321 ctccgagcaa tttctcaacc acttcaacca gttgatgatt cggtgggcat tcatagccag
    28381 ggatcggcgg atgcagctcg tcgaccgtca gacgacccgg ccagcgttcg ctcaccggag
    28441 ccaatgcatc gttgagcaaa ccattaagtt cattgagtgt catgctaggc agcggacgaa
    28501 tatccatatg caactcacag caagcgcaaa tacggttaga agcgtcgcca ccgtgaatat
    28561 gcccgaggtt gagcgtaggg tatggcacgg taaatgcttc gtagtgataa cgttctttca
    28621 ggttatcgcg caattgcaaa atatgcccga tggcgtcgtg cattagttcg atagcgttaa
    28681 ctccgcgtgc tggatcgctg gagtgccccg actggccctg aatacggatg gcgttagaga
    28741 tatgaccttt atgtgcgcgt accggttgta gtgacgtcgg ttcgccaatg atggcgcaat
    28801 ccgggcgcag ggcggtagtt tcggcaaaat aacgcgctcc ggccatactg gtttcttcat
    28861 cagcagtcgc cagaatgtag agcggttttt tcagtttcgt gacgtcgaca tcgcgtagcg
    28921 tatcaaggat aaacgcaaaa aagcctttca tgtcggcggt gcctaagccg taaagcttgc
    28981 cgtcatgctc cgtcagtgta aacggatcgc gcgtccagcg accgtcatca aatggcaccg
    29041 tatcggtatg ccccgccagc aacaagccgc cagccccctg tccgatactt gccagcatat
    29101 tgaatttgtt gcgagttcct ggcacaggct gcacttccac attgaagcct aaatctttaa
    29161 accagtccgc cagcagagtg attaaatctg cattgctttg atcgagtgcc tcttccgtgg
    29221 cgcttattga aggtgtggca atcagagcac ggtaaatctc gataaatggc ggtaatttgt
    29281 ttttcattgt tgacacacct ctggtcatga tagtatcaat attcatgcag tatttatgaa
    29341 taaaaataca ctaacgttga gcgtaataaa acccaccagc cgtaaggtga atgttttacg
    29401 tttaacctgg caaccagaca taagaaggtg aatagccccg atgttgaata cgctgattgt
    29461 gggtgccagc ggctacgctg gcgcagagct agtgacctat gtaaatcgcc atccgcatat
    29521 gaacataacc gctttgactg tttcagcgca aagcaatgat gcgggaaagt taatctccga
    29581 tttgcatccg cagctaaaag gcatcgttga gctgccgttg cagccgatgt cggatatcag
    29641 cgagtttagc ccaggggtgg acgtagtgtt tctcgccacc gcccatgaag ttagccacga
    29701 tttagcgccg cagtttcttg aagcgggctg cgtggtgttc gacctttccg gtgcgtttcg
    29761 tgttaacgac gccaccttct atgaaaaata ttacggcttt acccatcaat acccggaact
    29821 gctggaacag gcagcctacg gtctggcgga gtggtgcggt aataaattaa aagaagcgaa
    29881 tttgattgcg gtgccaggct gttatccgac ggcggcacag ctggcgctga aaccgttgat
    29941 tgatgccgat cttcttgacc tcaatcagtg gccggtgatc aacgccacca gcggcgtgag
    30001 cggtgcagga cgtaaggcag cgatttcaaa tagtttttgt gaagttagcc tgcaaccgta
    30061 tggcgtcttt actcatcgcc atcaaccaga gatcgccaca cacctcggtg ctgacgttat
    30121 cttcacccca catctgggca atttcccgcg cggcattctc gaaaccatta cctgccgcct
    30181 gaaatcgggt gtgacccagg cgcaagtcgc gcaagtgtta cagcaggcgt atgcccataa
    30241 accgctggtg cggctgtatg acaaaggcgt tccggcgctg aaaaatgtcg ttgggctgcc
    30301 attttgcgat atcgggtttg ccgttcaggg cgagcatctg attattgtgg cgaccgaaga
    30361 caacttactg aaaggcgcgg cggcacaagc ggtacagtgc gccaatattc gtttcggcta
    30421 tgcggaaacg cagtctctta tttaagggtg caatgatgaa tccattaatt atcaaactgg
    30481 gcggcgtact gctggatagt gaagaggcgc tggaacgtct gtttagcaca ctggtgaatt
    30541 atcgtgagtc acatcagcgt ccgctggtga ttgtgcacgg cggcggttgc gtggtggatg
    30601 agctgatgaa agggctgaat ctgccggtga aaaagaaaaa cggcctgcgg gtgacgcctg
    30661 ctgatcagat agacattatc accggagcac tggcgggaac ggcaaataaa accctgttgg
    30721 catgggcgaa gaaacatcag attgcggccg taggtttgtt tctcggtgac ggcgacagcg
    30781 tcaaagtgac ccagcttgat gaagagttag gtcatgttgg actggcgcag ccaggttcgc
    30841 ctaagcttat caactccttg ctggagaacg gttatctgcc ggtggtcagc tccattggcg
    30901 taacagacga agggcaactg atgaacgtca atgccgacca ggcggcaacg gcgctggcgg
    30961 caacgctggg cgcggatctg attttgctct ccgacgtcag cggcattctc gacggcaaag
    31021 ggcaacgcat tgccgaaatg accgccgcga aagcagaaca actgattgag cagggcatta
    31081 ttactgacgg catgatagtg aaagtgaacg cggcgctgga tgcggcccgc acgctgggcc
    31141 gtccggtaga tatcgcctcc tggcgtcatg cggagcagct tccggcactg tttaacggta
    31201 tgccgatggg tacgcggatt ttagcttaag ttttgttggc cggaggcgca gctttccggc
    31261 attgaatttc aaaataagga aacagagtta tggcactttg gggcgggcgt tttacccagg
    31321 cagcagatca acggttcaaa caattcaacg actcactgcg ctttgattac cgtctggcgg
    31381 agcaggatat tgttggctct gtggcctggt ccaaagccct ggtcacggta ggcgtgttaa
    31441 ccgcagaaga gcaggcgcaa ctggaagagg cgctgagcgt gctgctggaa gatgttcgcg
    31501 ccaggccaca acaaatcctt gaaagcgacg ccgaagatat ccatagctgg gtggaaggca
    31561 aactgatcga caaagtgggc cagttaggca aaaagctgca taccgggcgt agtcgtaacg
    31621 atcaggtagc gactgacctg aaactgtggt gcaaagatac cgttagcgag ttactgacgg
    31681 ctaaccgaca gctgcaatcg gcgctggtgg aaaccgcaca aaacaatcag gacgcggtca
    31741 tgccaggtta cactcacctg caacgcgccc agccggtgac gttcgcgcac tggtgcctgg
    31801 cctatgttga gatgctggcg cgtgatgaaa gccgtttgca ggatgcgctt aagcgtctgg
    31861 atgtcagccc attaggctgt ggcgcgctgg cgggaacggc ctatgaaatc gaccgtgaac
    31921 agttagcagg ctggctgggc tttgcttcgg cgacccgtaa cagtctggac agcgtttctg
    31981 accgtgacca tgtgttggaa ctgctgtcgg ctgccgctat cggcatggtg catctgtcgc
    32041 gttttgctga agatctgatt ttctttaaca ccggcgaagc ggggtttgtg gagctttctg
    32101 accgcgtgac ttccggttca tcattaatgc cgcagaagaa aaacccggat gcgctggagc
    32161 tgattcgcgg taaatgcggt cgggtgcagg gtgcgttaac cggcatgatg atgacgctga
    32221 aaggtttgcc gctggcttac aacaaagata tgcaggaaga caaagaaggt ctgttcgacg
    32281 cgctcgatac ctggctggac tgcctgcata tggcggcgct ggtgctggac ggcattcagg
    32341 tgaaacgtcc acgttgccag gaagcggctc agcagggtta cgccaacgcc accgaactgg
    32401 cggattacct ggtggcgaaa ggcgtacctt tccgcgaggc gcaccatatc gtgggtgaag
    32461 cggtggtgga agccattcgt cagggcaaac cgctggaaga tctgccgctc gacgagttgc
    32521 agaaattcag cccggtgatt ggcgaagatg tctatccgat tttgtcgctg caatcgtgcc
    32581 tcgacaagcg tgcggcaaaa ggcggcgtct caccgcagca ggtggcccaa gcgattgctt
    32641 ttgcgcaggc gcggttgggg taagaacatt tatatgtaca aatttgagcc tggcttatcg
    32701 ccgggctttt ttatggcaaa aaaaagcgga tcctggagat ccgcaaaagt tcacgttggc
    32761 tttagttatt cgagttgaga aactctcgaa acgggcagtg acttcaaggg ttaaaagagg
    32821 tgccgctccg tttctgtgag caattatcag tcagaatgct tgatagggat aatcgttcat
    32881 tgctattcta cctatcgcca tgaactatcg tggcgatgga ggatggataa tgaatattcg
    32941 tgatcttgag tacctggtgg cattggctga acaccgccat tttcggcgtg cggcagattc
    33001 ctgccacgtt agccagccga cgcttagcgg gcaaattcgt aagctggaag atgagctggg
    33061 cgtgatgttg ctggagcgga ccagccgtaa agtgttgttc acccaggcgg gaatgctgct
    33121 ggtggatcag gcgcgtaccg tgctgcgtga ggtgaaagtc cttaaagaga tggcaagcca
    33181 gcagggcgag acgatgtccg gaccgctgca tattggtttg attcccacgg ttggaccgta
    33241 cctgctaccg catattatcc cgatgctgca ccagaccttt ccaaagctgg aaatgtatct
    33301 gcatgaagca cagacccacc agttactggc gcaactggac agtggcaaac tcgattgcgt
    33361 gatcctcgcg ctggtgaaag agagcgaagc attcattgaa gtgccgttgt ttgatgagcc
    33421 aatgttgctg gctatctatg aagatcaccc gtgggcgaac cgcgaatgcg taccgatagc
    33481 cgatctggca ggggaaaaac tgctgatgct ggaagatggt cactgtttgc gcgatcaggc
    33541 aatgggtttc tgttttgaag ccggggcgga tgaagataca cacttccgcg cgaccagcct
    33601 ggagacgctg cgcaacatgg tggcggcagg tagcgggatc actttactgc cagcgctggc
    33661 tgtgccgccg gagcgcaaac gcgatggggt tgtttatctg ccgtgcatta agccggaacc
    33721 acgccgcact attggcctgg tttatcgtcc tggctcaccg ctgcgcagcc gctatgagca
    33781 gctggcagag gccatccgag caagaatgga tggctatttc gataaagtgt taaaacaggc
    33841 ggtttaaacc gtttaacgca gctacccgat aggcttccgc catggtcggg tagttaaagg
    33901 tggtgttgac gaagtactca atagtgttgc cgccaccttt ctgttccata atcgcctgac
    33961 cgatatgaat aatttcggca gcgcgctcgc caaagcagtg aatacccaga atctcttttg
    34021 tttcccgatg gaacaaaatt ttcagcgtgc ccacgttcat gccgacgatt tgtgcgcgtg
    34081 ccagatgttt aaactgggcg cggcccactt catatggcac tttcatcgcg gtcagctgct
    34141 gttcggtttt gcccacagag ctgatttccg ggatggtgta aatgccggta gggatatctt
    34201 caatcagatg cgcggtggct tcgcctttta ccagcgcctg cgcggcaatg cgcccctggt
    34261 cataggccgc tgatgccagg ctcggataac caatcacgtc gcccaccgcg taaacgtgcg
    34321 gctgtgcggt ctgatacatg ctgttgacct tcagctgtcc gcgactgtca gtttccagcc
    34381 caatgttctg taacgccagc gaatcggtat taccggtacg accgttggca tagagcaggc
    34441 agtcagcttt cagtttttta cccgacttca gatgcatgat cacaccgtcg tcacagcctt
    34501 cgatcttctc gtactcttcg ttgtgacgaa tcactacgcc actgttccag aagtgatagg
    34561 agagagaatc tgacatctct tgatcgagaa atgccagcag gcgatcgcgg gtgttgatca
    34621 gatccacttt tacatccata ccgcggaaga tcgacgcata ttcacagccg atcactccag
    34681 caccatagat aagtacatgg cgcggttcgt ggtgcatgct aagaattgag tcgctgtcgt
    34741 aaatgcgtgg atgggtgaaa tcaacatctg ttggatgata tggacgagag ccgcaggcaa
    34801 taacaaattt ttcagcggtt agtgtttcga cgctgccgtc cgggcaatcc agcgccaacg
    34861 tatgctcgtc aacaaagcga gcgttcccct gcaatatttc acagtgatta cgttcgtaaa
    34921 atccctgacg catgcgcgtt tgttgattaa tcacgttatc ggcatggtta aggatatcgg
    34981 caaaagaaga gcggagcagt cgcgaatggt cgctgtatag tgggttttga ttgaattcta
    35041 taatgcggct gacggcgtga cggagagctt tcgacgggat ggtgccccag tgggtgcaac
    35101 cgccgccaac attttgataa cgctcgataa ctgcgacgcg cacaccttgc ttaaccaggc
    35161 ccattgcagc gccttcgccg ccggggccgg aacctattac tatggcatcg taatcgtagg
    35221 aatgtggcat ggtagggctt acctgttctt atacataaaa gcaacagaat ggtaacattc
    35281 tatcgcgggt aagccaattg atccccgtca tttatctggc tatatcctga gcggcctttg
    35341 ctttgtctgt ttcttacttt tgccctgacg ttttattgga tttttatcga cgatactctc
    35401 cgtttaagcg gcaggtttcc gctgtacgta aaagaaccgg ccagtaaata tgttttattg
    35461 cgttaccgtt cattcacaat actggagcaa tccagtatgt tcattctctg gtatagtgcc
    35521 agcagtactt ttggcaagga ttcagacatc gtgatgggca taagagcgca acaaaaagaa
    35581 aaaacccgcc gttcgctggt ggaagccgca tttagccaat taagtgctga acgcagcttc
    35641 gccagcctga gtttgcgtga agtggcgcgt gaagcgggca ttgctcccac ctctttttat
    35701 cggcatttcc gcgacgtaga cgaactgggt ctgaccatgg ttgatgagag cggtttaatg
    35761 ctacgccaac tcatgcgcca ggcgcgtcag cgtatcgcca aaggcgggag tgtgatccgc
    35821 acctcggtct ccacatttat ggagttcatc ggtaataatc ctaacgcctt ccggttatta
    35881 ttgcgggaac gctccggcac ctccgctgcg tttcgtgccg ccgttgcgcg tgaaattcag
    35941 cacttcattg cggaacttgc ggactatctg gaactcgaaa accatatgcc gcgtgcgttt
    36001 actgaagcgc aagccgaagc aatggtgaca attgtcttca gtgcgggtgc cgaggcgttg
    36061 gacgtcggcg tcgaacaacg tcggcaatta gaagagcgac tggtactgca actgcgaatg
    36121 atttcgaaag gggcttatta ctggtatcgc cgtgaacaag agaaaaccgc aattattccg
    36181 ggaaatgtga aggacgagta atgaaacaag caaatcaaga tagaggtacg ctgctgctgg
    36241 cgttagttgc tggcttatcg attaatggta ctttcgcagc gctgtttagc tccattgtgc
    36301 cattttctgt attcccgatt atttccctgg tgctgacggt ttactgcctg catcaacgtt
    36361 atcttaatcg cactatgccg gtaggcttgc cgggtctggc agctgcctgt tttattctcg
    36421 gcgtactgct gtacagcacg gtagttcgtg cggaatatcc ggatatcggc tctaacttct
    36481 tcccggcagt actctccgtc attatggtgt tctggattgg cgcgaagatg cgtaaccgta
    36541 agcaggaagt tgctgagtaa tcggtattat gccggatgct ggcgcatccg gcatgggttt
    36601 tacttcgcgg tcagtaatac gccgcactcc atatggtgcg tgtaggggaa ctgatcaaac
    36661 agagccagac gttcgacctt gtgcgtctgg cttaatgttt ccagattctt gcataacgtt
    36721 tccgggttac aggagatgta caaaatacac ggatacgcct gcaccatttt ctcggtttca
    36781 ctgtccagac cgctgcgcgg tgggtcgaca aaaatggttt cgcactgata actctttaag
    36841 tcgatccctt gcaggcggtt aaactcgcgc acaccattca tcgcctgagt aaattcttct
    36901 gccgccatac gaataatttg tacgttatca atatggttag ctgcgatgtt gtattgcgca
    36961 gcagcaaccg acggcttagc gatttcggtg gctaataccc gatcaaaatt gcgcgccagc
    37021 gctaatgaaa agttaccgtt gccgcagtac agctccagta aatcgccttt tgagcctttg
    37081 gttacgtcca gcgcccattc cagcatctga atattcatcg ccgcgttcgg ctgggtaaag
    37141 ctgttttcta cctgacggta gatcatctct ttccctgcga ccggcagacg ctcgtcgatg
    37201 taatcttgat ccagctcgat tttggttttc gttgcccgac caatcagatg cacattcaga
    37261 ttctgcgcgc gcagtgcatc gcgcagggcc tccgcttcct gacgccactc atcatccagc
    37321 ttcttatggt atagcaggga aaccaccgcc tgattactca gcgtggtgag gtaatcaatc
    37381 tggaacaact tgtggcgcag aacgggatta ttacgcacac ccgcaatcat cgccgtcatc
    37441 aactggttga taagttcact ggcggcgggg aagctatcca cgcggatacg gcttttggtt
    37501 tgttgatcga aaatgatgtg atacaggtca tcgccatcgt gccagatgcg gaactccgca
    37561 cgcatccggt aatgactgac cggcgagcga aacacttccg gaaccaggtc agaaaacggt
    37621 gccatcatac tttgcaaacg taccactttt tcggctaact gcgcttcata ctgttctgtt
    37681 ggaaggtgtt cgggggtcat gatgtatcct gaaaaattaa agtacgcggc gattgtaggg
    37741 attgctcatc agatgtccag atcttgatga attcctattt gtgagctacg tctggacagt
    37801 aacttgttac aacctgtagc atccacttgt cggtcctgtg agttaatagg gaatccagtg
    37861 cgaatctgga gctgacgcgc agcggtaagg aaaggtgcga tgattgcgtt atgcggacac
    37921 tgccatccgg tgggaagtca tcatctctta gtatcttaga tacccctcca agcccgaaga
    37981 cctgccggcc aacgtcgcat ctggttctca tcatcgcgta atattgatga aacctgcggc
    38041 atccttcttc tattgtggat gctttacaat gattaaaaaa gcttcgctgc tgacggcgtg
    38101 ttctgtcaca gccttttccg cttgggcaca ggataccagc ccggatactc tcgtcgttac
    38161 tgctaaccgt tttgaacagc cgcgcagcac tgtgcttgca ccaaccaccg ttgtgacccg
    38221 tcaggatatc gaccgctggc agtcgacctc ggtcaatgat gtgctgcgcc gtcttccggg
    38281 cgtcgatatc acccaaaacg gcggttcagg tcagctctca tctattttta ttcgcggtac
    38341 aaatgccagt catgtgttgg tgttaattga tggcgtacgc ctgaatctgg cgggggtgag
    38401 tggttctgcc gaccttagcc agttccctat tgcgcttgtc cagcgtgttg aatatatccg
    38461 tgggccgcgc tccgctgttt atggttccga tgcaataggc ggggtggtga atatcatcac
    38521 gacgcgcgat gaacccggaa cggaaatttc agcagggtgg ggaagcaata gttatcagaa
    38581 ctatgatgtc tctacgcagc aacaactggg ggataagaca cggggaacgc tgttgggcga
    38641 ttatgcccat actcatggtt atgatgttgt tgcctatggt aatacaggaa cgcaagcgca
    38701 gacagataac gatggttttt taagtaaaac gctttatggc gcgctggagc ataactttac
    38761 tgatgcctgg agcggctttg tgcgcggcta tggctatgat aaccgtacca attatgacgc
    38821 gtattattct cccggttcac cgttgctcga tacccgtaaa ctctatagcc aaagttggga
    38881 cgccgggctg cgctataacg gcgaactgat taaatcacaa ctcattacca gctatagcca
    38941 tagcaaagat tacaactacg atccccatta tggtcgttat gattcgtcgg cgacgctcga
    39001 tgagatgaag caatacaccg tccagtgggc aaacaatgtc atcgttggtc acggtagtat
    39061 tggtgcgggt gtcgactggc agaaacagac tacgacgccg ggtacaggtt atgttgagga
    39121 tggatatgat caacgtaata ccggcatcta tctgaccggg ctgcaacaag tcggcgattt
    39181 tacctttgaa ggcgcagcac gcagtgacga taactcacag tttggtcgtc atggaacctg
    39241 gcaaaccagc gccggttggg aattcatcga aggttatcgc ttcattgctt cctacgggac
    39301 atcttataag gcaccaaatc tggggcaact gtatggcttc tacggaaatc cgaatctgga
    39361 cccggagaaa agcaaacagt gggaaggcgc gtttgaaggc ttaaccgctg gggtgaactg
    39421 gcgtatttcc ggatatcgta acgatgtcag tgacttgatc gattatgatg atcacaccct
    39481 gaaatattac aacgaaggga aagcgcggat taagggcgtc gaggcgaccg ccaattttga
    39541 taccggacca ctgacgcata ctgtgagtta tgattatgtc gatgcgcgca atgcgattac
    39601 cgacacgccg ttgttacgcc gtgctaaaca gcaggtgaaa taccagctcg actggcagtt
    39661 gtatgacttc gactggggta ttacttatca gtatttaggc actcgctatg ataaggatta
    39721 ctcatcttat ccttatcaaa ccgttaaaat gggcggtgtg agcttgtggg atcttgcggt
    39781 tgcgtatccg gtcacctctc acctgacagt tcgtggtaaa atagccaacc tgttcgacaa
    39841 agattatgag acagtctatg gctaccaaac tgcaggacgg gaatacacct tgtctggcag
    39901 ctacaccttc tgaaccacgt cccaccgtgc tggtgtttga ctccggcgtc ggtgggttgt
    39961 cggtctatga cgagatccgg catctcttac cggatctcca ttacatttat gctttcgata
    40021 acgtcgcttt cccgtatggc gaaaaaagcg aagcgtttat tgttgagcga gtggtggcaa
    40081 ttgtcaccgc ggtgcaagaa cgttatcccc ttgcgctggc tgtggtcgct tgcaacactg
    40141 ccagtaccgt ttcacttcct gcattacgcg aaaagttcga cttcccggtt gttggtgtcg
    40201 tgccggcgat taaacctgct gcacgtctga cggcaaatgg cattgtcgga ttactggcaa
    40261 cccgcggaac agttaaacgt tcttatactc atgagctgat cgcgcgtttc gctaatgaat
    40321 gccagataga aatgctgggc tcggcagaga tggttgagtt ggctgaagcg aagctacatg
    40381 gcgaagatgt ttctctggat gcactaaaac gtatcctacg cccgtggtta agaatgaaag
    40441 agccgccaga taccgttgta ttgggttgca cccatttccc tctactacaa gaagaactgt
    40501 tacaagtgct gccagaggga acccggctgg tggattctgg cgcagcgatt gctcgccgaa
    40561 cggcctggtt gttagaacat gaagccccgg atgcaaaatc tgccgatgcg aatattgcct
    40621 tttgtatggc aatgacgcca gaagctgaac aattattgcc cgttttacag cgttacggct
    40681 tcgaaacgct cgaaaaactg gcagttttag gctgatttgg ttgaatgttg cgcggtcaga
    40741 aaattatttt aaatttcctc ttgtcaggcc ggaataactc cctataatgc gccaccactg
    40801 acacggaaca acggcaaaca cgccgccggg tcagcggggt tctcctgaga atctcaacag
    40861 agaaaagcaa aaaaatgctt gactctgt
//
LOCUS       DAJVRF010000017        40725 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJVRF010000017 DAJVRF010000000
VERSION     DAJVRF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40725
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.017"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..1592
                     /gene="asnB"
                     /locus_tag="OQN13_000847"
     CDS             <1..1592
                     /gene="asnB"
                     /locus_tag="OQN13_000847"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCS1614288.1"
                     /translation="RLMRHRGPDWSGIYASDNAILAHERLSIVDVNAGAQPLYNQQKT
                     HVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEVILALYQEKGPEFLDDLQGMFAFALY
                     DSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVASEMKSLVPVCRTIKEFPAGSYLWSQD
                     GEIRSYYHRDWFDFDAVKDNVTDKNELRQALEDSVKSHLMSDVPYGVLLSGGLDSSII
                     SAITKKYAARRVEDQERSEAWWPQLHSFAVGLPGSPDLKAAQEVANHLGTVHHEIHFT
                     VQEGLDAIRDVIYHIETYDVTTIRASTPMYLMSRKIKAMGIKMVLSGEGSDEVFGGYL
                     YFHKAPNAKELHEETVRKLLALHMYDCARANKAMSAWGVEARVPFLDKKFLDVAMRIN
                     PQDKMCGNGKMEKHILRECFESYLPASVAWRQKEQFSDGVGYSWIDTLKEVAAQQVSD
                     QQLETARFRFPYNTPTSKEAYLYREIFEELFPLPSAAECVPGGPSVACSSAKAIEWDE
                     AFKKMDDPSGRAVGVHQSAYK"
     gene            1972..2048
                     /locus_tag="OQN13_000848"
     tRNA            1972..2048
                     /locus_tag="OQN13_000848"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2006..2008,aa:Met,seq:cat)
     gene            2057..2141
                     /locus_tag="OQN13_000849"
     tRNA            2057..2141
                     /locus_tag="OQN13_000849"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2091..2093,aa:Leu,seq:tag)
     gene            2165..2239
                     /locus_tag="OQN13_000850"
     tRNA            2165..2239
                     /locus_tag="OQN13_000850"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2197..2199,aa:Gln,seq:ttg)
     gene            2274..2348
                     /locus_tag="OQN13_000851"
     tRNA            2274..2348
                     /locus_tag="OQN13_000851"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2306..2308,aa:Gln,seq:ttg)
     gene            2364..2440
                     /locus_tag="OQN13_000852"
     tRNA            2364..2440
                     /locus_tag="OQN13_000852"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2398..2400,aa:Met,seq:cat)
     gene            2489..2563
                     /locus_tag="OQN13_000853"
     tRNA            2489..2563
                     /locus_tag="OQN13_000853"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2521..2523,aa:Gln,seq:ctg)
     gene            2600..2674
                     /locus_tag="OQN13_000854"
     tRNA            2600..2674
                     /locus_tag="OQN13_000854"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2632..2634,aa:Gln,seq:ctg)
     gene            complement(2828..4003)
                     /gene="ubiF"
                     /locus_tag="OQN13_000855"
     CDS             complement(2828..4003)
                     /gene="ubiF"
                     /locus_tag="OQN13_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCS1614289.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            4149..5573
                     /gene="miaB"
                     /locus_tag="OQN13_000856"
     CDS             4149..5573
                     /gene="miaB"
                     /locus_tag="OQN13_000856"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCS1614290.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            5726..6766
                     /locus_tag="OQN13_000857"
     CDS             5726..6766
                     /locus_tag="OQN13_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCS1614291.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            6763..7230
                     /gene="ybeY"
                     /locus_tag="OQN13_000858"
     CDS             6763..7230
                     /gene="ybeY"
                     /locus_tag="OQN13_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCS1614292.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            7320..8198
                     /gene="corC"
                     /locus_tag="OQN13_000859"
     CDS             7320..8198
                     /gene="corC"
                     /locus_tag="OQN13_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCS1614293.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            8223..9761
                     /gene="lnt"
                     /locus_tag="OQN13_000860"
     CDS             8223..9761
                     /gene="lnt"
                     /locus_tag="OQN13_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCS1614294.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(9839..11716)
                     /locus_tag="OQN13_000861"
     CDS             complement(9839..11716)
                     /locus_tag="OQN13_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCS1614295.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVPAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            12171..13079
                     /gene="gltI"
                     /locus_tag="OQN13_000862"
     CDS             12171..13079
                     /gene="gltI"
                     /locus_tag="OQN13_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCS1614296.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            13249..13989
                     /gene="gltJ"
                     /locus_tag="OQN13_000863"
     CDS             13249..13989
                     /gene="gltJ"
                     /locus_tag="OQN13_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCS1614297.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            13989..14663
                     /gene="gltK"
                     /locus_tag="OQN13_000864"
     CDS             13989..14663
                     /gene="gltK"
                     /locus_tag="OQN13_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCS1614298.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            14663..15388
                     /gene="gltL"
                     /locus_tag="OQN13_000865"
     CDS             14663..15388
                     /gene="gltL"
                     /locus_tag="OQN13_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCS1614299.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            15506..16441
                     /gene="rihA"
                     /locus_tag="OQN13_000866"
     CDS             15506..16441
                     /gene="rihA"
                     /locus_tag="OQN13_000866"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCS1614300.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            16533..18203
                     /gene="hscC"
                     /locus_tag="OQN13_000867"
     CDS             16533..18203
                     /gene="hscC"
                     /locus_tag="OQN13_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCS1614301.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(18263..19717)
                     /gene="djlC"
                     /locus_tag="OQN13_000868"
     CDS             complement(18263..19717)
                     /gene="djlC"
                     /locus_tag="OQN13_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCS1614302.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDDEEEREAFLEA
                     IQAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAK
                     LHRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLC
                     AYWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTR
                     LLWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVV
                     LEAFPKQEMLARLNTRLEGGDTHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKN
                     DEDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(19714..20421)
                     /locus_tag="OQN13_000869"
     CDS             complement(19714..20421)
                     /locus_tag="OQN13_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1614303.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEERKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            20523..21077
                     /locus_tag="OQN13_000870"
     CDS             20523..21077
                     /locus_tag="OQN13_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1614304.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(21087..22514)
                     /locus_tag="OQN13_000871"
     CDS             complement(21087..22514)
                     /locus_tag="OQN13_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCS1614305.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLLSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(22511..23218)
                     /locus_tag="OQN13_000872"
     CDS             complement(22511..23218)
                     /locus_tag="OQN13_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1614306.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            23382..24358
                     /locus_tag="OQN13_000873"
                     /pseudo
     CDS             23382..24358
                     /locus_tag="OQN13_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
     gene            complement(24428..24910)
                     /locus_tag="OQN13_000874"
     CDS             complement(24428..24910)
                     /locus_tag="OQN13_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCS1614307.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            25145..27727
                     /gene="leuS"
                     /locus_tag="OQN13_000875"
     CDS             25145..27727
                     /gene="leuS"
                     /locus_tag="OQN13_000875"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCS1614308.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            27742..28323
                     /gene="lptE"
                     /locus_tag="OQN13_000876"
     CDS             27742..28323
                     /gene="lptE"
                     /locus_tag="OQN13_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCS1614309.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            28323..29354
                     /gene="holA"
                     /locus_tag="OQN13_000877"
     CDS             28323..29354
                     /gene="holA"
                     /locus_tag="OQN13_000877"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCS1614310.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVTARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            29356..29997
                     /gene="nadD"
                     /locus_tag="OQN13_000878"
     CDS             29356..29997
                     /gene="nadD"
                     /locus_tag="OQN13_000878"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCS1614311.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            30021..30632
                     /locus_tag="OQN13_000879"
                     /pseudo
     CDS             30021..30632
                     /locus_tag="OQN13_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            30892..31209
                     /gene="rsfS"
                     /locus_tag="OQN13_000880"
                     /gene_synonym="rsfA"
     CDS             30892..31209
                     /gene="rsfS"
                     /locus_tag="OQN13_000880"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCS1614312.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            31213..31680
                     /gene="rlmH"
                     /locus_tag="OQN13_000881"
     CDS             31213..31680
                     /gene="rlmH"
                     /locus_tag="OQN13_000881"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCS1614313.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            31711..33612
                     /gene="mrdA"
                     /locus_tag="OQN13_000882"
     CDS             31711..33612
                     /gene="mrdA"
                     /locus_tag="OQN13_000882"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCS1614314.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            33615..34727
                     /gene="mrdB"
                     /locus_tag="OQN13_000883"
                     /gene_synonym="rodA"
     CDS             33615..34727
                     /gene="mrdB"
                     /locus_tag="OQN13_000883"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCS1614315.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            34738..35826
                     /gene="rlpA"
                     /locus_tag="OQN13_000884"
     CDS             34738..35826
                     /gene="rlpA"
                     /locus_tag="OQN13_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCS1614316.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            35965..37176
                     /gene="dacA"
                     /locus_tag="OQN13_000885"
     CDS             35965..37176
                     /gene="dacA"
                     /locus_tag="OQN13_000885"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCS1614317.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            37286..37549
                     /locus_tag="OQN13_000886"
     CDS             37286..37549
                     /locus_tag="OQN13_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCS1614318.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            37650..38291
                     /gene="lipB"
                     /locus_tag="OQN13_000887"
     CDS             37650..38291
                     /gene="lipB"
                     /locus_tag="OQN13_000887"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCS1614319.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            38551..39504
                     /locus_tag="OQN13_000888"
     CDS             38551..39504
                     /locus_tag="OQN13_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCS1614320.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            39713..40678
                     /gene="lipA"
                     /locus_tag="OQN13_000889"
     CDS             39713..40678
                     /gene="lipA"
                     /locus_tag="OQN13_000889"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCS1614321.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT        10202 a        10379 c        10248 g         9896 t
ORIGIN      
        1 cacgcctgat gcgtcatcgt ggcccggact ggtccggtat ttatgccagc gataacgcca
       61 ttctcgctca cgaacgtctg tcaattgttg acgttaacgc gggggcgcaa cctctctaca
      121 accaacaaaa aacccacgta ctggcggtaa acggtgaaat ctacaaccac caggcattgc
      181 gcgctgaata tggcgatcgt tatcagttcc agacagggtc tgactgtgaa gtgatcctcg
      241 cgctgtatca ggaaaaaggg ccggaatttc tcgacgactt gcagggcatg tttgcctttg
      301 ccctgtacga cagcgaaaaa gatgcctacc tgattggtcg cgaccatctg gggatcatcc
      361 cactgtatat ggggtatgac gaacacggtc agctgtatgt ggcctcagaa atgaaatcgc
      421 tggtgccagt ttgccgcacg attaaagagt tcccggcggg gagctatttg tggagccagg
      481 acggcgaaat ccgttcttat tatcatcgcg actggttcga ctttgatgca gtgaaagata
      541 acgtgaccga caaaaacgag ctgcgtcagg ctctggaaga ttccgttaaa agccatctga
      601 tgtctgatgt gccttacggt gtgctgcttt ctggtggtct ggattcctca attatttccg
      661 ctatcaccaa gaaatacgca gcccgtcgcg tggaagatca ggaacgctct gaagcctggt
      721 ggccccagtt acactccttt gctgtaggtc tgccgggttc accggatctt aaagcagccc
      781 aggaagtggc aaaccatctg ggcacggtgc atcacgaaat tcacttcact gtacaggaag
      841 gtctggatgc catccgcgac gtgatttacc acatcgaaac ttatgatgtg accactattc
      901 gcgcttcaac accgatgtat ttaatgtcgc gtaagatcaa ggcgatgggc attaaaatgg
      961 tgctgtccgg tgaaggttct gatgaagtgt ttggcggtta tctttacttc cataaagcgc
     1021 ccaacgctaa agaactgcat gaagagacgg tgcgtaaact gctggccctg catatgtatg
     1081 actgcgcgcg cgccaacaaa gcgatgtcag cctggggcgt ggaagcacgc gttccgttcc
     1141 tcgacaaaaa attccttgat gtggcgatgc gcattaaccc gcaggataaa atgtgcggta
     1201 acggcaaaat ggaaaaacac atcctgcgtg aatgttttga gtcatacctg cccgcaagcg
     1261 tggcctggcg gcagaaagag cagttctccg atggcgtcgg ttacagttgg atcgacaccc
     1321 tgaaagaagt ggcggcgcag caggtttctg atcagcaact ggaaactgcc cgcttccgct
     1381 tcccgtacaa cacgccaacc tcaaaagaag cgtatctgta ccgggagatc tttgaagaac
     1441 tgttcccgct tccgagcgcc gctgagtgcg tgccgggcgg cccgtccgtc gcgtgttctt
     1501 ccgctaaagc gattgagtgg gatgaggcgt tcaagaaaat ggacgatccg tctggtcgtg
     1561 cggttggcgt tcaccagtcg gcgtataagt aagaaatgcg aaacagcccc ggtgaaatcc
     1621 ggggctgttt cagttattga ggtgcttgtt gattctgata tgcagtaagg aaatgttccg
     1681 ggtttgccac atcactgttt gacaactcat cactgtaggc cagtatttca tagattaatt
     1741 tcccaccacc attgttgaat tccgaatttc atcctgtatg acaaaagaat ggctgtaggg
     1801 tggggaaaaa acacaaagta aacaataatt gacgaatata gcgccacgct gttcgcaacc
     1861 taaccaaaca gtcactttcg agcaattttc cttgaaaaag aggttgacgc tgcaaggctc
     1921 tatacgcata atgcgccccg caacgccgat aaggtatcgc gaaaaaaaga tggctacgta
     1981 gctcagttgg ttagagcaca tcactcataa tgatggggtc acaggttcga atcccgtcgt
     2041 agccaccatc ttttttgcgg gagtggcgaa attggtagac gcaccagatt taggttctgg
     2101 cgccgcaagg tgtgcgagtt caagtctcgc ctcccgcacc attcaccaga aagcgttgta
     2161 cggatggggt atcgccaagc ggtaaggcac cggtttttga taccggcatt ccctggttcg
     2221 aatccaggta ccccagccat cttcttcgag taagcggttc accgaccggt tattggggta
     2281 tcgccaagcg gtaaggcacc ggtttttgat accggcattc cctggttcga atccaggtac
     2341 cccagccatc gaagaaacaa tctggctacg tagctcagtt ggttagagca catcactcat
     2401 aatgatgggg tcacaggttc gaatcccgtc gtagccacca aattctgaat gtgtcgaata
     2461 cgttcggcaa attcaaaaac caatttgttg gggtatcgcc aagcggtaag gcaccggatt
     2521 ctgattccgg cattccgagg ttcgaatcct cgtaccccag ccaatttatt caagacgctt
     2581 acatcgtaag tgcaccagtt ggggtatcgc caagcggtaa ggcaccggat tctgattccg
     2641 gcattccgag gttcgaatcc tcgtacccca gccacattaa aaaagctcgc ttcggcgagc
     2701 tttttgcttt tctgcgttca ttcaatgtcg aatgcgatgt tgacacgtct tatccttcaa
     2761 tgtcggatgc gacgctgccg cgtcttatcc gacctacggt tggcacgcat ccggcaatgt
     2821 tgtaaggcta caaccctaac gcatatttca gcgcctgacg tttcaacacg ccagcacgct
     2881 ctgccgccat taacccgaga ttacgcacaa aacgcagcgg cggcagatta ttactgaatc
     2941 cggcataaaa cagatccata ccactttgca taatgaagtt atccgccatg cgccgcatct
     3001 ggtaacgctt gaggacagga taactggccc acgcttcacc gtagctgcgg gcattcacca
     3061 gaacatcaat cagggcatcg acatcacgat aaccaagatt caccccctgc cccgccagcg
     3121 gatggatggt atgcgcggca tcgcccacca gcgcaagccc cggctgcaca tactgcaacg
     3181 catgtcggcg cgtcagcgga aacgcaccag cggcaagcgg tgtaacgtaa cccagacgcg
     3241 acgggaaatg cttcgcgatt tccgcctgga attgcgccat attcatattc tgcaactggc
     3301 gaatacgcgc cggagagtca taccacacca gcgatgccca gttatcaaac aacggcagaa
     3361 acgcacgcgg tccgtccggg gtaaattgct gccaggtgct gtcgccggga tcgttctcac
     3421 actgtacgct aatcaacatg cacgactgcg catactgcca ggcatgaacg ccaattcccg
     3481 ccatctgccg cacctgagaa tttgcgccgt cggcaccaat caccagtttc gcgcggatcg
     3541 tttcaccgcc cttcagttcc agctcctgaa gatcattatc tcgatgtaat gcaatcagcg
     3601 agcctggcac acgtaacgtt actttcggat gcgcttccag cgcctgccac agcgcctgtt
     3661 gcaggacggt gttttccacc atatagccaa gcaacggtag cttaagttca gcggcgtcaa
     3721 acaccacatg cgccgtttcc cactcccacg tttccagtct gcggtaagga tggcaacgca
     3781 tagcctgtac tgcatcccag acccctaacc ctttaagcaa tgataccgat gcggcgctga
     3841 tcgccgagat ccgcacgtcc ggttggctat cagcgacaaa cggtgcaggt tctgcgtgct
     3901 cgatcaccgt taccgaaaat ccgtgctgtg ccagccccag cgccagcgcg ccgccgacca
     3961 ttcctccgcc gacaatggca atttccgttg gttgatttgt catggtcaat catcctgtta
     4021 gcaatatgct ttaagtttac aggattttta cccccaaggg ctgataacgc tcatactggt
     4081 cacaacggca gcaaagcatt acactatacg ccctgcattc ctggctacta tttcgcaaga
     4141 gcaagtcgat gaccaaaaaa ctccatatta aaacctgggg ctgtcagatg aacgagtacg
     4201 attcatcgaa gatggccgat ctgctggatg ccacccacgg ctatcaactg accgacgtgg
     4261 cggaagaagc ggatgtgctg ctgctgaaca cctgctcaat ccgcgagaag gctcaggaaa
     4321 aagtcttcca tcagttgggt cgctggaaac tgttaaaaga gaagaatcca gacctgatta
     4381 tcggcgtcgg tggctgcgtg gcatcgcaag aaggcgagca cattcgccag cgcgcccact
     4441 atgtcgatat tatttttggg ccgcaaacgc tgcaccgtct gccggagatg atcaactccg
     4501 tgcgcggcga ccgcagcccg gttgtagata tcagcttccc ggaaatcgag aagtttgacc
     4561 gtctaccgga accgcgcgcc gaagggccga ccgcgtttgt ctccatcatg gaaggctgca
     4621 ataaatattg cacctactgc gtggtgcctt acacccgtgg tgaagaggta agccgtccgt
     4681 ctgacgatat tctgtttgag attgcccagc ttgcggctca gggcgtgcgt gaagtcaacc
     4741 tgctcggtca gaacgtgaac gcctggcgtg gtgaaaacta cgacggctcc accggatcgt
     4801 ttgccgatct gctgcgtctg gttgctgcga tcgacgggat cgatcgtatt cgctttacca
     4861 ccagccatcc gatcgaattc accgacgata tcatcgaagt gtatcgcgac acgccggagc
     4921 tggtgagctt cctgcatctg ccagtacaaa gcggttccga tcgcattctg aacctgatgg
     4981 gccgtactca cacggcgctg gagtacaaag cgatcatccg taaactgcgt gcggcgcgtc
     5041 cggatattca gatcagttct gacttcattg ttggcttccc tggcgaaacc accgaagact
     5101 tcgagaaaac gatgaagctg attgccgacg tcaatttcga catgagctac agctttatct
     5161 tctctgcacg tccgggtaca ccagccgccg atatggttga tgatgttccg gaagaagaga
     5221 agaagcaacg tctgtatatt ctgcaagaac gtatcaatca gcaagcgatg gcatggagcc
     5281 gccggatgct cggcaccacc cagcgtattc tggtagaagg gacttcgcgt aagagcatca
     5341 tggagctttc cgggcgtacc gaaaataacc gcgtggtcaa cttcgagggc acaccggata
     5401 tgatcggtaa attcgtcgat gtagaaatta ccgacgtcta cccgaactct ctgcgcggta
     5461 aagtggtgcg tactgaagat gaaatgggtc tgcgcgtggc agaaacaccg gaatcggtga
     5521 ttgcccgtac ccgcaaagaa aacgaccttg gcgtgggtta ttatcagccg taattctcag
     5581 gcccgccgtt ccggtgggcc ttgttttttc tctctttatc cccatcttta tcgcaatgct
     5641 tgcgcctttt tcctgtggcg tgaataattt ccttataggc cggtgaatgt tcagcttccg
     5701 gcggcataca gataagagga acggtttgaa catagacact cgcgaaatca ccctggagcc
     5761 agcagacaac gcgcgtctgt tgagcctgtg cggcccgttt gatgacaaca tcaagcagct
     5821 cgaacgccgt ctcggcatcg agatcaatcg ccgcgacaac cactttaaac tgaccggccg
     5881 tccgatttgc gtcaccgctg cggcagacat tctgcgtagc ctgtatgtcg atacagcccc
     5941 gatgcgcggt cagattcagg atatcgaacc ggaacagatc caccttgcga ttaaagaagc
     6001 gcgagtactg gagcaaagtg cggagagcgt gccggagtac ggcaaagcgg tcaatatcaa
     6061 aaccaaacgc ggcgtaatta agccgcgcac gccaaaccag gcgcagtaca tcgccaatat
     6121 tctcgaccat gacattacct tcggcgttgg cccggcgggt acgggtaaaa cctacctggc
     6181 agtggctgcg gcagttgatg ccctggagcg tcaggagatt cgccgtattc tgctgactcg
     6241 tccggcggtc gaagccggtg agaaactggg cttcctgcct ggcgatttaa gccagaaagt
     6301 agacccgtat ctgcgcccac tgtacgacgc gctgtttgaa atgctgggct ttgagaaagt
     6361 tgagaaactg attgagcgca acgttattga agtcgcgccg ctggcctata tgcgtggtcg
     6421 tacgctgaac gacgcgttta tcattctcga tgagagccag aacaccacca tcgaacagat
     6481 gaagatgttc ctgacccgta tcggttttaa ctcaaaagcg gttatcaccg gcgacgtcac
     6541 acagatcgac ctgccgcgta atactaaatc aggcttacgt cacgccatcg aagtgctggc
     6601 cgatgtcgaa gagatcagct ttaacttctt ccacagcgaa gacgtggttc gtcaccctgt
     6661 ggtggcgcgt atcgttaacg cctatgaagc ctgggaagaa gccgaacaaa aacgaaaagc
     6721 ggcgctggcg gcagaacgca agcgcgaaga acaggaacaa aaatgagtca ggtgatcctc
     6781 gatttacaac tggcatgtga agataattcc gggttaccgg aagagagcca gtttcaaaca
     6841 tggctgaatg cggtgatccc gcagtttcag gaagaatcgg aagtgactat tcgcgtggtc
     6901 gataccgccg aaagccacag tctgaatctg acctatcgcg gtaaggataa gccgaccaac
     6961 gtgctctcct tcccgtttga agtgccgcct ggcatggaaa tgtcgctact gggcgatctg
     7021 gttatctgcc gtcaggtggt tgagaaggaa gctcaggagc aaggcaaacc actggaggcg
     7081 cactgggcgc atatggtggt gcacggcagt ctgcatttgt taggttacga tcacatcgaa
     7141 gatgacgaag cagaagaaat ggaagccctc gaaacagaga ttatgcttgc tctgggctat
     7201 gaggatccgt acattgccga gaaagaataa tttgccaggc atttgactgg cagacgtccc
     7261 cgccgttggt gattaacgac gacgttttga ttaactaaca tgagaaccca tacgacgcca
     7321 tgagcgacga caattcacac agtagtgaca cgataagcaa caagaaggga tttttctccc
     7381 tgttactcag ccaacttttc cacggtgaac cgaaaaaccg tgacgaactg ctggcgctga
     7441 tccgtgattc cgggcagaac gaccttatcg acgaagatac gcgcgatatg ctcgaagggg
     7501 tgatggacat cgcagaccaa cgcgttcgcg acatcatgat cccccgctcc cagatgatta
     7561 ccctgaaacg caaccagacg ctggacgaat gccttgatgt catcatcgag tccgcccact
     7621 cacgtttccc ggtgattagc gaagacaaag atcacattga agggattctg atggcgaaag
     7681 acttgctgcc gtttatgcgc agcgatgctg aagccttcag catggacaaa gtgttacgtc
     7741 aggcggttgt cgttcctgaa agtaagcgcg tagaccggat gctgaaagag tttcgctctc
     7801 agcgttacca catggcgatc gtcattgacg aattcggtgg ggtttccggt ctggtgacca
     7861 ttgaagacat cctggaactg attgttggtg agattgaaga cgagtatgac gaagaagatg
     7921 atatcgactt ccgtcagctg agtcgtcata cctggaccgt gcgtgcactg gcttccattg
     7981 aagacttcaa cgaagcgttc ggcacccact ttagcgatga agaggttgac actatcggtg
     8041 gtctggtgat gcaggcattt gggcatcttc cggcgcgtgg cgaaactatc gacatcgacg
     8101 gttaccagtt caaagtggcg atggccgaca gtcggcgtat tattcaggtt catgtcaaaa
     8161 tcccggatga ctcaccccag ccgaagctgg atgaataaaa ccgaaactgg atagataact
     8221 acatggcttt tgcctcatta attgaacgcc agcgcattcg cctgctgctg gcgttattat
     8281 tcggtgcctg cggaacgctg gccttctctc cttacgacgt ctggcctgcg gcgattattt
     8341 cgttgatggg gcttcaggcg ttgaccttta accgccgtcc actccagtct gccgctattg
     8401 gcttttgctg gggatttggc ctctttggca gcggtattaa ctgggtctat gtcagcatcg
     8461 cgacctttgg cggaatgcct ggcccggtta acatcttcct ggtggtgctg ctggcggcgt
     8521 atttgtcgct gtataccgga ctgtttgctg gcgtgctgtc gcgtctgtgg ccgaaaacca
     8581 cctggctgcg cgtggcgatt gccgcccctg ctctctggca agtgaccgag tttctgcgcg
     8641 gttgggtact gacaggcttc ccgtggttac agttcggcta tagccagatt gatggcccgt
     8701 taaaagggct ggcaccgata atgggcgtgg aagccattaa cttcctgctg atgatggtta
     8761 gtggcctgct ggcactggcg ttggtcaaac gcaactggcg tccgctggtg gtggccgtcg
     8821 tgctgtttgc cctacccttc ccgctgcgtt acatccaatg gtttaccccg caaccggaga
     8881 aaaccattca ggtttcgatg gttcagggcg atattccgca atcactgaaa tgggatggag
     8941 atcagcttct taatacgctg aagatttact acaacgcaac ggctccgctg atgggcaaat
     9001 catcgttgat tatctggccg gagtcggcga taaccgatct ggaaattaat cagcaaccgt
     9061 tcctcaaagc actggacggt gagttgcgcg ataaaggtag ctcgctggta accgggattg
     9121 tcgacgcgcg tctcaataag cagaaccgct acgataccta caacaccatc atcacactgg
     9181 gtaaaggtgc accatacagc tacgaatcag ccgatcgcta taacaaaaac catctagtgc
     9241 cgtttggcga gtttgtcccg ctggagtcga ttctgcgtcc gttagcaccg ttctttgatc
     9301 tgccgatgtc gtcgttcagc cgtgggccat atatccagcc gccgctgtcg gtaaatggta
     9361 ttgagcttac tgcggctatt tgctacgaga tcattctcgg cgagcaagtg cgcgataact
     9421 tccgcccgga taccgactat ctgctgacta tctccaacga tgcgtggttt ggtaagtcta
     9481 ttggtccatg gcagcacttc cagatggcgc gaatgcgtgc gctggagctg gcgcgcccac
     9541 tgttgcgcag caccaacaac ggcattacgg cggtgattgg cccgcagggg gaggttcagg
     9601 cgatgatccc gcagttcacc cgcgaggtgt tgaccactaa tgtgacaccg accaccggac
     9661 tcacaccgta tgcacgtacc ggcaactggc cactgtgggt gctgacggcg ctgtttggtt
     9721 ttgccgctgt gttgatgagt ctgcgagcac gtaaacgttg atgtgcctga tgtatcctgc
     9781 atgagcaaaa cgtactttct cattgagctt tggggcatgg ttatcatgcc cctttttctt
     9841 acggttcagg gatacaagcc acattgccat attggggatg acgaataatt gcctgcggat
     9901 caagcgagcg cgtcctgtcc atttggatca acccgttctt cgccatgaca aatatttttc
     9961 taccatccgc tgaaaaacga aggtgattaa cgtagctggc aggataaacg ccaataatct
    10021 gcccggcatt aatattgagc aggatgacat aaccttgcag gccatatgac accaacgcta
    10081 aatattgacc gtccgggcta ataccctcaa cggaataaac gctcctgcta caccattgcg
    10141 tcataatgct ggcttgttga tcccctacgc ttatacgtga aatcccctga ctatcggcaa
    10201 aaataacctc gccgttttca ggtcgaaaac gcatcgccga tatcgttcca cgcgcatcac
    10261 ctgcgggaaa gtcttctttc tgatgcgtga tggtattgat ccgcacgatg ttacctctgc
    10321 ctgctcccgg aaccagtaat gttctttcat ccggggaaag tgccataacc caggcgcctg
    10381 gtttaccgct cgtccccgtc ccgtcgtatt tctccagcgg gatatccgcc acatgctgat
    10441 aagtcaccag atcaatcgct ggcacactat tactggtgcg attcatcacc caggcttttg
    10501 tgccatcacg cgagaggata aggctgtcag gatgtgcacc ggtggtaatc gactgaataa
    10561 tctcccctgt cgccacgtta atgaccgata acgcattcgc ttcaaatgaa gtcgcataaa
    10621 tcaacttttc atccgggctt aatgccagcg atctgacgcc ctgatcttta cagccaccac
    10681 aatgtttgac tgacttttct ttcgctatgg gcgctgtaaa ggtatgcagg attttctttt
    10741 gtccgaggtc gaaaacaaca atgctgttcg tgttttcaat tgcggcatac aacatattac
    10801 catcccgact gatcgccata tcatggatat cggcaatctc agcgagcaaa tcaccactgg
    10861 cctgttcacg acttaccgga gcggataatg tttccctgag ggcgttgcgt tcctccgcta
    10921 actgctgctt tttttgccgt cgctcttgtt caatctcctg tggataaaac gcttcacgca
    10981 ggctttgcct gacctgtaaa actgactcat ccatctgctg ctgcgcaagc cagatagccc
    11041 cggtcagaag aagactgccc gcaacaataa tcccgccttc cgtaacgagt cggttttgcc
    11101 cagataaacg cgcgctcagc caacccaacg cgccacccgc aatcaggccg ccaatgtggc
    11161 aagcgttatc tattcctgtt tgccgggcac cgtacaacag tgtcagcgcc accataccca
    11221 acagaggaaa tactcgccgc tggtttttat ggtatgtgcc cgtacctgaa gcaaactgtg
    11281 ttgctatcgc tgcaccagct attcccagaa tcgccccgga tgccccaacg ctgatgacta
    11341 tctgctcact ttcgcgtaac gcataacagg cgctgatgag cccaccaaag acgccggaga
    11401 ataaccaggt aatcaggagt cgccatttcc ccagaatctg ttccgccacg cctcccacga
    11461 ccaggaatgc aaacatattc atcagcagat gagaaacact actgtgcaga aagatactgc
    11521 tcaataagcg ccagggttca cccgacaaag taagaggcgc aacgtttgcg ccgagatgga
    11581 taagtgcgga ttgatcgacc tcttcataac tcatccattg tgcgagataa caaaatatcc
    11641 caatacagag tgcaaaaagg ataagggtta ttgcgggtaa ttgaacattt aacggcttaa
    11701 ccgaagatgc agacataagg tgtttcttcc ttgaaaagac aggtaaaaag catttttccc
    11761 gtgtgcagtt atcatcccgg caaacgtccc atgccgaaaa gataattctg tccgaaatat
    11821 gactttttca gcctaaaatt acaatcgtaa ttaattcgcc cctaagtatt ttttggcatg
    11881 taagttgctt tatttactgt gtaagcgatg aaatcagccg cgacgcaacc aggttgcacc
    11941 accacaaatc agcggtagca ccaaaaaggt gcggtttaag aattttgctt cggaatggtg
    12001 cggcgcatct cgcaaaaaca aacaataaaa cagcaatatc tttacattta gccaaactaa
    12061 atgttaccta acaggcacaa cactgcacaa taaagttgca gacgataaca acacaaacac
    12121 tcacaacggg tatccatgcg ttcttaacgc agaagataaa ggagttggat atgcaattac
    12181 gtaaacctgc cacagcaatc ctcgccctgg cgctttccgc aggactggca caggcagatg
    12241 acgccgcccc ggcagcaggc agtacgctgg acaaaatcgc caaaaacggt gtgattgtcg
    12301 tcggtcaccg tgaatcttca gtacctttct cttattacga caatcagcaa aaagtggtgg
    12361 gttactcgca ggattactcc aacgccattg ttgaagcagt gaaaaagaaa ctcaacaaac
    12421 cggacttgca ggtaaaactg attccgatta cctcacaaaa ccgtattcca ctgctgcaaa
    12481 acggcacttt cgattttgaa tgtggttcta ccaccaacaa cgtcgaacgc caaaaacagg
    12541 cggctttctc tgatactatt ttcgtggtcg gtacgcgcct gctgaccaaa aagggcggcg
    12601 atatcaaaga ttttgccgac ctgaaagaca aagccgtagt cgtcacttcc ggcactacct
    12661 ctgaagtttt gctcaacaaa ctgaatgaag agcaaaaaat gaatatgcgc atcatcagcg
    12721 ccaaagatca cagtgactct ttccgcaccc tggaaagcgg tcgtgccgtt gcctttatga
    12781 tggatgatgc cctgctggcc ggtgaacgtg cgaaagcgaa gaaaccagac aactgggaaa
    12841 tcgtcggcaa gccgcagtct caggaagcct acggttgcat gttgcgtaaa gacgatccgc
    12901 agttcaaaaa gctgatggat gacaccaccg cgcaggtgca gacctccggt gaagcggaaa
    12961 aatggtttga taaatggttc aaaaatccca ttccgccgaa aaacctgaac atgaatttcg
    13021 aactgtcaga cgaaatgaaa gcactgttca aagaaccgaa tgacaaggca ctgaactaat
    13081 tacaagaacc aggggcggaa attccagccc tctcgattgt tacgtagcac ggacagacta
    13141 tacgcctgat ggtcgttccc catcgggcct gaaaaccgca atacgctggg taacaatctt
    13201 cgagggtagc agttaacgct gctaccctct tttttctgga gtagatttat gtctatagac
    13261 tggaactggg gtattttttt acaacaagcc ccgttcggca acaccaccta tcttggttgg
    13321 atctggagtg gttttcaggt gacgatcgct ttgtcgatct gcgcctggat tatcgctttc
    13381 ctcgtcggtt catttttcgg cattttacgt accgttccaa accgttttct ctccggtctg
    13441 ggtacgttgt atgtcgaact gttccgcaac gtgccgttga ttgtgcaatt ctttacctgg
    13501 tatctggtga tcccggagct gttgccggag aaaatcggca tgtggtttaa ggccgagctg
    13561 gatcccaata ttcagttttt cctttcatcc atgctctgcc tggggctgtt taccgccgcc
    13621 cgtgtttgcg aacaggtacg cgccgccatt cagtcgttgc cgcgtgggca aaaaaatgcc
    13681 gcgctggcga tgggcctgac gctaccgcag gcttatcgat acgtactgct gcctaatgct
    13741 tatcgcgtta tcgtcccgcc gatgacttcc gagatgatga acctggtgaa aaactccgcc
    13801 atcgcctcga ctattggtct ggtggatatg gcggcgcagg cgggtaaatt gcttgattac
    13861 tcagcccacg cctgggagtc gtttaccgct attacgctgg cgtatgtgtt gattaacgcg
    13921 gttatcatgc tggtaatgac gctggttgaa cgtaaagttc gcctgcctgg caacatgggg
    13981 ggcaaataat gtacgagttt gactggagtt ccattgtccc ttccctgcca tatctgctcg
    14041 acgggctggt gatcacgctg aaaatcaccg tcacggcagt cgtgattggt attttgtggg
    14101 gcaccatgct ggcggtgatg cgcttgtcca gctttgcgcc cgtagcgtgg tttgccaaag
    14161 cctacgttaa cgtattccgc tcgattcctt tagttatggt tttgctgtgg ttttacctga
    14221 tcgtgccagg ttttctgcaa aacgtgctgg gattatcacc aaaaaatgat atccgtttaa
    14281 tttcggcgat ggtggcgttt tcgatgtttg aagcggccta ctattcagag attatccgtg
    14341 ctggtattca aagtatttct cgtggtcagt cgagcgccgc gctggcgttg ggaatgactc
    14401 actggcagtc gatgaaactg attattctgc cgcaggcgtt ccgcgcgatg gtgccgcttc
    14461 tgctcactca gggcatcgta ctgttccagg atacctcact ggtgtatgtg ttatctctgg
    14521 ccgatttctt ccgtaccgcc tcaaccattg gtgagcgtga tggtacgcag gttgagatga
    14581 tcctgtttgc cggatttgtt tatttcgtta ttagtcttag cgcgtcgttg ttggtcagct
    14641 acttgaaaag aaggacagca taatgattac cctgaaaaat gtttcaaaat ggtatggtca
    14701 ctttcaggtg ctgaccgact gctcaaccga agtgaaaaaa ggcgaagtgg tggtggtttg
    14761 cggcccgtct ggttccggca aatcaacgct gattaaaacc gtcaacggcc tcgaaccagt
    14821 gcagcaaggt gaaatcaccg tcgatggtat cgtggttaat gacaagaaaa ccgatctggc
    14881 aaagctgcgt tcccgcgtcg ggatggtatt ccagcatttc gagctgttcc ctcatctgtc
    14941 gattatcgaa aacctgaccc tggcgcaggt gaaagtgctt aaacgcgata aagcgcccgc
    15001 tcgtgaaaaa gcattgaaat tgctggagcg cgtgggactt tccgcacatg ccaataaatt
    15061 tccggcgcag ctttccggcg gtcagcagca gcgtgtggca atcgctcgcg cattgtgtat
    15121 ggatcctatt gcgatgctgt tcgacgaacc gacatcggcg ctggatccgg agatgatcaa
    15181 cgaagtgctg gacgtgatgg tggaactggc gaacgaagga atgaccatga tggtggtgac
    15241 ccacgaaatg ggctttgccc gcaaagtggc gaatcgggtg atctttatgg acgagggtaa
    15301 aattgtcgaa gactcgccga aagacgcttt cttcgatgat ccgaaatcgg accgcgcgaa
    15361 agacttcctc gcgaaaatcc tgcattaatc actctggcgc gcatacccaa gtgcgcgcca
    15421 tctcacgctt tatcgtttcg cccctcgtat ctgttacatc ctggcgttac ccttgtcgca
    15481 aagaagcaca caacaaggag caacaatggc actgccaatt ctgttagatt gcgacccagg
    15541 tcatgacgac gctatcgcaa tagttctcgc cctcgcctca ccagagcttg atgtcaaagc
    15601 aattacgtct tccgccggaa accagacacc agaaaaaacc ttacgcaatg ttctgcgtat
    15661 gctgaccttg cttaatcgca ccgatattcc ggtagcaggc ggcgcggtaa aaccgttaat
    15721 gcgtgagttg attatcgcgg acaatgtgca cggcgaaagc ggtctggatg gcccggcatt
    15781 accggaaccg acgttcgcac cgcaaaactg tacggcggta gagctgatgg cgaaaacgct
    15841 gcgcgaaagt gcggaacctg tcacgattgt gtctaccgga ccgcaaacta acgttgcctt
    15901 gctgctcaat agccatccgg aactgcatag caaaattgcc cgtatcgtga ttatgggcgg
    15961 cgcaatgggg cttggtaact ggacgcctgc ggctgaattt aatatttacg ttgacccgga
    16021 agcggcagaa attgtcttcc agtcagggat cccagtggtg atggccggtc tggatgttac
    16081 tcacaaagca caaatccacg ttgaagacac cgagcgtttc cgcgcgattg gcaaccctgt
    16141 ttcaaccatt gttgccgaac tgctggattt cttcctcgaa tatcataaag acgaaaaatg
    16201 gggctttgtc ggtgcaccgc tgcatgaccc atgcaccatc gcctggctgt tgaaacccga
    16261 gttgtttacc actgttgagc gctgggttgg cgtggaaaca caggggaaat atagccaggg
    16321 tatgacggtt gttgattatt attatctgac tggcaataaa ccgaatgcca ctgtaatggt
    16381 cgatgttgat cgtcagggct ttgttgattt actggctgat cgtctgaaat tttacgctta
    16441 acacacgtca ttgattaaga tcaccacgcg ttacctgggt ggtcttaatc tgtttatctg
    16501 tttatctctt tttctttcaa gatataaatg caatggataa tgcagaactc gccattggta
    16561 tcgatctcgg cactaccaat agtttaattg ccgtctggaa agacggtgcc gcgcaattaa
    16621 ttccaaataa gttcggtgaa tatttaacac catccataat tagcatggat gaaaataatc
    16681 atattttagt cggaaaaccg gctgtatcac ggcgtacttc gcatccggat aaaacggcag
    16741 cgttatttaa acgtgcaatg ggcagtaata ccaactggcg gttaggcagc gacacattta
    16801 acgcgccaga actgtcctct ttggtattac gctcattaaa agaagatgcc gaagaatttc
    16861 tgcaacgtcc gattaaagat gtggtgatct ccgttccggc ttatttcagc gatgaacaac
    16921 gcaagcatac ccgtttagca gcggagttag ccgggttaaa tgcggtacgc ttaattaatg
    16981 aacccacagc agctgcgatg gcatatggcc tgcatacgca acaaaatacc cgctcgctgg
    17041 tttttgatct cggtggcggc acgtttgacg tcacggtgct cgaatacgcc acgccggtga
    17101 ttgaagttca cgcctccgct ggcgataact tcctcggtgg cgaagatttt acccatatgt
    17161 tggtcgatga cgttttaaag cgcgcggatg tcgccaggac cacgcttaac gagagtgaac
    17221 tggcagcctt gtacgcctgt gtggaagcgg caaaatgtag caatcaatcg ccattgcaca
    17281 ttcgctggca gtaccaggat gaaacgcggg agtgcgaatt ttacgagaac gaactggaag
    17341 atttgtggtt gccgctgctc aatcgcttgc gagtgccgat tgaacaggcg ttgcgcgatg
    17401 cacgtctgaa gccgagtcaa atcgacagtc tggtgctggt tggcggcgcg tcacaaatgc
    17461 cgctggtgca gcgaatcgcc gtgcgtctgt ttggcaaatt accgtatcaa agttacgacc
    17521 cgagcaccat tgtcgcgctg ggcgcagcaa tccaggccgc ctgccgctta cgcagtgaag
    17581 atattgaaga ggtgatcctc actgatattt gcccttactc gttgggcgtt gaagttaacc
    17641 gccagggcgt ttccggcatt ttctcgccaa ttattgaacg aaacaccaca gtacccgtgt
    17701 cgcgtgtaga aacttactcg accatgcacc cggaacagga ttctattacg gttaacgtct
    17761 atcagggaga aaaccacaaa gttaaaaata acattctggt tgaatccttc gatgtgccgt
    17821 tgaagaaaac cggggcttat cagtcgattg atattcgctt tagttatgat atcaacggct
    17881 tgcttgaagt tgacgtgctt ctggaagatg gcagcgttaa gtccagagtc attaatcata
    17941 gcccggtaac attgagcgcg cagcagattg aagagagtcg gacgcggtta tccgcattga
    18001 aaatttatcc gcgcgatatg ctaatcaacc gcacctttaa ggccaaactg gaagagttgt
    18061 gggcgcgggc gctgggtgac gagcgagaag agattggccg ggtgatcacc gattttgatg
    18121 cggcgttgca gtcaaacgat atggcccgcg tcgatgaagt tcgtcgacgg gcgagcgatt
    18181 atttggctat tgagatccct taaacacggc tgccggatgc ggcataaacg ctgcatccgg
    18241 catcggcgca aaaaacacat cctcagctaa acaacatcgc gatttttccg agcgcataac
    18301 ctatcatcag caccgttaag ctgattttga taatactggt aatgctctgt ggtttttcat
    18361 cctcatcatt tttttcttca tggttttcag gtgttggctc atcgacatct tcatcctccg
    18421 gctcgagttt cactttcgtc aacagcatct gggtgataat ggcctctaat ggatgagtat
    18481 ctccaccttc cagccttgta ttaagacggg ccagcatctc ttgtttcggg aacgcttcca
    18541 gcacaacctg ttccgctacc cgatacaaat ccagtaacgg tgaatcttgc tctgtctcgc
    18601 taccgactct cgatttccac cacgcccatg ctttgcgctt gaagaataac gactcaataa
    18661 acgattgacc taacggcgat aattttccgc tatgtaaaat ctccgcccat aacagccgcg
    18721 tccgggcgct ctcaccagga acatatttca acggtttacc ttgcgcatct accatgcttc
    18781 gcgcttcgat aaccatcact aataacgcga aataatccgg gcaatgacgc ttgctccact
    18841 gcaacaacaa attatcagct tgtgtagatg ggtaactttc ccagtacgcg cagagatcgg
    18901 caagcaggct ttccccctcg ccgcagtaca aacgttgtgc acactggtaa taccgggcat
    18961 cttcgctttc tggttcttcc gcttgccact gtttggctac aggaatgagt tccgccatac
    19021 ctgtttgcac cgagctgtac cagcgcaaca gtttgcgatg taactttgca tcatcaggaa
    19081 tgaaccacgg tccttccatc gccaaaaacg cagtgacata gtcaggatgg taacggcagc
    19141 aacgttccag cgcgaagtaa tattcaacag tctggttctg taacgcgacg ggatattcca
    19201 gaaggctaag gaaatcgaaa caatcaccag cctgaatggc ctcaagaaaa gcctcccttt
    19261 cttcctcatc atccattcct tcgctgtcat taaacgaatg ccagttcaaa cgctctgcca
    19321 acaaagaagc acaatttaat gaaagatatc gcgcttctat agcgattgca cacagcggcc
    19381 agcgtaattg atcgacatcc tccatgttcc aggtatttaa ttgctgaata aatttctgcc
    19441 aggcggaggg ctgaaaacga tcactttctg aatccagtaa tgtcctgaat gcacgtagta
    19501 tttcatgttc agcggtggca tctttttctt catcatcggc ttcctcgaca ggatttaccg
    19561 ccagtcgcag cgcctcttca taggcctggc gtaatgcttt aaacccctgc ggatcggttt
    19621 cgggatgaca caacggtaag cgagcaagat aagcctggcg gataatgtct atttgcgtcg
    19681 tgctttcaat ttcgaggatt tgccaacatg ttttcatagg tcgctcacat ccttaagcgt
    19741 taccgggcat tcaggctgga cagttaatgg ttgccaggga agcgtggcat agaaacggtc
    19801 gcttcccgct gccagctctt cgaacatccg actcccggcg ctatcgtatt tgtacagggt
    19861 tagcgcctcg cgtagcgaag cgttgtcttc gcactgagaa gattgccagt agagacgccc
    19921 tagtgaatag gcagaaaaat agtgcatcca gctgtgatag taatggtgag ctcgtgcata
    19981 gacgcgcgat tgcagccata aactctcttc ttccgtcagc cagttgttga gcacaccgac
    20041 ccgtgacaga aaccccattc gaacataatc ccaggctttg attccccctt ctccacagca
    20101 catagcggtt tgggcaacga attgggtata aatcaaccct ctctccgagc caccagcaat
    20161 taatgctttc ctctcttcag gggaagagcg ttgccatgtc agatataagc cagctaaatc
    20221 attggcatga ccatcatcag tcatctgata aatagtctga attaaatcat cacgattatt
    20281 tattccccag gatgatgaga gatcgaattc ttttatccca gtatacattt ttggcgagtc
    20341 atgcgcgggt ttgcaactct gattaagtat ttccatgggc gcagaaagcg caaaaagaag
    20401 atattgttct tccttattca ttttttaata atcccttaag tgcattaact gactactttt
    20461 ttattattga tgagtataaa cttgacgctt tcaaaataaa aagaaaatcg aagcattcac
    20521 acatgaataa aaaatcaatg tatatattcg caatttttat agttgcagca attacctgta
    20581 ttagccaacc caagaaaacg acgttgcgtg ataaagccat ggtgaattat gcctttgatt
    20641 atttaagatc accaggcagt ctaccgttaa ccacggcagc cacggagctt tccgcgattc
    20701 atggtcactc aacatcgcaa tatcgccttg gcgaatttta tcttcatggt agcgacggta
    20761 aaccactgga ttatacacag gcgagatact ggtatgagca atcagcagaa caggaaaatc
    20821 cgcgcgcaca aagcaaactg gggtggatct acctgaaagg cctgggagtc aaacccgata
    20881 cccgtaaagc aattctctgg tataaggaag cagctgaaca agggtatgct catgctcaat
    20941 atactttggg tttgatctac agaaatggca ctggtattaa tgttaaccat tatgaatctc
    21001 aaaaatggtt aaaactggcc gccaaacaac attacaaaaa tgcggaaaga ttacttgccg
    21061 gacttcccgc acattaacgt cgactgtcac ccgaagagat ggagtatttt tcctatcaaa
    21121 ccggcaaaga taaaaatgta gaaaataatt ttgatgacgc ccagacagcc cagcccgcca
    21181 tcgtcaacgg gcattggctg cggttgcggc tccggctcga cggtttcctg accggtaaaa
    21241 ttcgccgcct gaattaataa tcgacgaata attaatgctt ccagtggaca acgatcttct
    21301 gcatcaaggc ggctcacaat cggcattaat gattcttctg tcggttggct ggcctgcact
    21361 aattgtttgg caactaaata taatggccat aagggatgtt ctgtatcacg cgtgagtgag
    21421 atccgcggct tacgtccttt gtgcaaaacc atctcgacca gtgaacgccc cagcgcagag
    21481 agtttttcat cgctgagtat ttcgtataaa tgaaaacgcg tacgggcgct gtcgccgggg
    21541 acataaagta gctgtttacc ctgatcgtta accagttcac gagcttcaat catcatcacc
    21601 actaacgggt aataatcgac ccgatgctga cggcaccatt gcaatattaa agcatccgcc
    21661 tgggtggagg gatactcgcg ccagtagtca caaagttctg ccagcaagct ttcgccttct
    21721 ccacaataga cgcgttgcgc gtattcataa taacgcgcat cttcattatc aggttcagct
    21781 tcccgccacg accgggcgta atccagaagt tcggggatat ccaggcgacc tgcgctatac
    21841 cagcggagta aatttctgtg cagttttgca tcatcgggga tgatcactgc gcgatgttgc
    21901 gctaaataca atgtcagcca ttctggatgc gatgaccaaa tccgagcgaa catttggtaa
    21961 aaatcgatgg tgtcattttg cacggcaacg ggcagatgca gaatggtctg gaagttaaaa
    22021 acattcccct tcgcaatggc atataaaaag gattccagtt cttcttcgtc tatttccccg
    22081 acgttttcct cctgcaacca ccgcaatctt tctgctaata acaccacgca ctcgaaggat
    22141 aaatgggcag tgttcatggc gattgtgcac agcgaccagc gtaattcatc aatctcttcc
    22201 atcgagcaat aatttaattg ctgaatgaat cgctgccagg cggagggcag aaaacgttcg
    22261 ctatcagagg caagtaacgc gcgaaaggcg agcagaattt catgttctgc tacctcatat
    22321 tcttctggtt gccaaacaga tttagcaggc gactgcgcaa tccggagcgc ctcctcatac
    22381 gcctgacgaa gttgtttaaa accctgcgga tcggtttctg gatgaaagga cagtaacagc
    22441 gccagataag cgcggcggat aatatcgacg tcagtcgttt cctctatttc gaggatcttc
    22501 cagcaatttt tcataggtcg ctcatatcct taagcgtttc cgggcatgtg gggtagtcag
    22561 ccagtggttg ccaggacaag gcagggtaat acgcatcatc actggcaaat aattgataaa
    22621 acatatcatt gccggaatcg tcgtattctt tacgagctaa gaattcgcgt aataaaggca
    22681 gatgatcatc ttcgggagac tgccaatatt gccgaccaaa ggtataaccg gcaaagtatt
    22741 gccgccagtt actataataa cgtagagccc gtagatgaat acgcgactgg atccatagac
    22801 tttcttcttc actcaaccag ttattaagta cgcccattcg acttaaaaaa cccatccgga
    22861 cataatccca tgctttaatt cctccctcac cacagcattc ggctgtgctg gcgacaaatt
    22921 gcgcataagc ctgaccttgc tcatttagtt cagcaagata gtcacgccat tcacatggcg
    22981 aataacgaaa ccagcggtga taaaaccctg ccaaccgagc ggcatgaccg ttatcgatca
    23041 ttcggtgaat tgtgcgcagc aggtcatcac ggtcatcaat tccccatgag gaagaaaggt
    23101 caaaaaagca tgtacctaaa tacattttgg gggatgaatg cgaaggtaaa cagcattcat
    23161 tgcgtatttc catcggagtg gaaagtgcaa acaatatttt ttgcgacccc atgtccatat
    23221 aatttatcct taaacattca tttaatgaat gtaaactaca tttcgagact cagttattta
    23281 tacttttctg acgcaacata atacgcataa aataaggctt acgcaaaaat ggaacacata
    23341 aaaatataat atgttttatc tacaaccata aggaaatgat aatggttttt acgtcaagtt
    23401 gctgcgataa tttatcaata gatgagatta tcgaacgtgc tgaaaaaggc gattgcgagg
    23461 ctcaatatat tgttgggttt tattataatc gcgatagcgc aattgactct ccggacgacg
    23521 aaaaggcctt ttactggctg aagctggccg ctgagcaagg tcattgtgaa gcacagtatt
    23581 ccttagggca aaagtatacc gaggataaaa gccgtcataa agataatgag caagccatct
    23641 tctggctgaa aaaagctgcc ctacaaggcc atactttcgc ttccaacgcc cttggctgga
    23701 cactggatcg tggagaagcc cccaattata aagaagcggt tgtctggtat cagatagccg
    23761 cggagagcgg aatgtcttat gcgcaaaata atcttgggtg gatgtacaga aatggcaacg
    23821 gagtcgcaaa agactatgcg ctggcatttt tctggtacaa acaagctgca ttacaaggcc
    23881 atagtgacgc gcaaaacaat ctggccgatc tttatgaaga cggaaaaggc gttgctcaaa
    23941 acaagacact cgccgcattc tggtatttga aaagcgcaca gcagggtaat cggcacgccc
    24001 agtttcaaat tgcatgggat tataacgctg gcgaaggggt ggaccaggac tataagcaag
    24061 cgatgtactg gtatctgaag gctgccgctc aggaaagcgt cggcgcttac gtcaacatcg
    24121 gttatatgta taaacacgga caaggcgttg agaaagatta tcaggctgcc tttgaatggt
    24181 ttacgaaagc cgctgaatgc aatgacgcca ctgcctggta taacctggcc attattatca
    24241 ctacggagaa ggaagacctg tcgatctccg acaggctctc gacctgtatc gtaaagttca
    24301 gtcatccgga accagagatg tcagtcagga aattcgtgag actgaagatt tactgtagag
    24361 ataaaaacaa gggccgggga atttcattcg gcccatattc tctttattgc tgtgcgaaat
    24421 cagactatta cggctcaaac gggcgtctct ggaactggtc atgaccacat ttcgggcata
    24481 gcgtcagcac ttccggtgtg tagatcggga gatggaagtg acatttctcg cagaccagat
    24541 ttcccagccc gaccacttct ccgctgtgat aaaccccatg atgattgagg tcctggaaaa
    24601 cttcgcgcca ttcaagctgc gttttatcgg tgatatctgc cagctcctgc cataagcttt
    24661 ctttaatcac ccgcataaag acgctgtcag attcttcttt caggctctct tcatagctca
    24721 tggcgaactc ttccaggtca cgtcttacag ctcgcgtcag ctcatcgacc tcggttcgcg
    24781 ttaactcccc tgtttttatt acgcgctcgc gcgcctgttc caccagtgcg tcgatatcac
    24841 gttcgccatt gcgcaggcgt tcgctcagtg acgcgaccag ttcacggtaa tattgagcaa
    24901 ccttgttcat cgtttcatct cccgggtcgt taactgacta taataataga cgttctttgt
    24961 tccttgcttt gctaatacgc ccacaaactc tgtaaattaa tgtatgaata atccgggcat
    25021 catttcaacg tcgagtgcgc gaaaggctgt tttgacgcgg acgtttgggc tatgctatgc
    25081 ggatctgaaa aaccacctca acgctacatt tgtagccgta ttgaaaacag gaccactggc
    25141 tgccatgcaa gagcaatacc gcccggaaga gatagaatcc aaagtacagc ttcattggga
    25201 tgagaagcgc acatttgaag taaccgaaga cgagagcaaa gagaagtatt actgcctgtc
    25261 tatgcttccc tatccttctg gtcgactaca catgggccac gtacgtaact acaccatcgg
    25321 tgacgtgatc gcccgctacc agcgtatgct gggcaaaaac gtcctgcagc cgatcggctg
    25381 ggacgcgttt ggtctgcctg cggaaggcgc ggcggtgaaa aacaacaccg ctccggcacc
    25441 gtggacgtac gacaacatcg cgtatatgaa aaaccagctc aaaatgctgg gctttggtta
    25501 tgactggagc cgcgagctgg caacctgtac gccggaatac taccgttggg aacagaaatt
    25561 cttcaccgag ctgtataaaa aaggcctggt atataagaag acttctgcgg tcaactggtg
    25621 cccgaacgac cagaccgtac tggcgaacga acaagttatc gacggctgct gctggcgctg
    25681 cgataccaaa gttgaacgta aagagatccc gcagtggttt atcaaaatca ctgcttacgc
    25741 tgacgagctg ctcaacgatc tggataaact ggatcactgg ccagacaccg ttaaaaccat
    25801 gcagcgtaac tggatcggtc gttccgaagg cgtggagatc accttcaacg ttaacgacta
    25861 tgacaacatg ctgaccgttt acactacccg cccggacacc tttatgggtt gtacctacct
    25921 ggcggtagct gcgggtcatc cgctggcgca gaaagcggcg gaaaataatc ctgaactggc
    25981 ggcctttatt gacgaatgcc gtaacaccaa agttgccgaa gctgaaatgg cgacgatgga
    26041 gaaaaaaggc gtcgatactg gctttaaagc tgttcaccca ttaacgggcg aagaaattcc
    26101 cgtttgggca gcaaacttcg tattgatgga gtacggcacg ggcgcagtta tggcggttcc
    26161 gggtcacgac cagcgcgact acgagtttgc ctctaaatac ggcctgaaca tcaaaccagt
    26221 tatcctggct gctgacggct ctgagccaga tctttctcag caagccctga ctgaaaaagg
    26281 cgtgctgttc aactctggcg aattcaacgg tcttgaccat gaagcggcct tcaacgccat
    26341 cgccgataaa ctgactgcga tgggcgttgg cgagcgtaaa gtgaactacc gcctgcgcga
    26401 ctggggtgtt tctcgtcagc gttactgggg cgcgccgatt ccgatggtga cgctggaaga
    26461 cggtaccgta atgccgaccc cggacgacca gctgccggtg atcctgccgg aagatgtggt
    26521 gatggacggc attaccagcc cgattaaagc agatccggag tgggcaaaaa ctaccgttaa
    26581 cggtatgcca gcgctgcgtg aaaccgacac tttcgacacc tttatggagt cctcctggta
    26641 ctatgcgcgc tacacttgcc cagagtacaa agaaggtatg ctggattccg aagcggctaa
    26701 ctactggctg ccggtggata tctacattgg tggtatcgaa cacgccatta tgcacctgct
    26761 ctacttccgc ttcttccaca aactgatgcg tgatgcaggc atggtgaact ctgacgaacc
    26821 agcgaaacag ttgctgtgtc agggtatggt gctggcagat gccttctact atgttggcga
    26881 aaacggcgaa cgtaactggg tttccccggt tgatgctatc gttgaacgtg acgagaaagg
    26941 ccgtatcgtg aaagcgaaag atgcggcagg ccatgaactg gtttataccg gcatgagcaa
    27001 aatgtccaag tcgaagaaca acggtatcga cccgcaggtg atggttgaac gttacggcgc
    27061 ggacaccgtt cgtctgttta tgatgtttgc ttctccggct gatatgactc tcgaatggca
    27121 ggaatccggt gtggaagggg ctaaccgctt cctgaaacgt gtctggaaac tggtttacga
    27181 gcacacagca aaaggtgatg ttgcggcact gaacgttgat gcgctgactg aagatcagaa
    27241 agcgctgcgt cgcgatgtgc ataaaacgat cgctaaagtg accgatgata tcggccgtcg
    27301 tcagaccttc aacaccgcaa ttgcggcgat tatggagctg atgaacaaac tggcgaaagc
    27361 accaaccgat ggcgagcagg atcgcgctct gatgcaggaa gcgctgctgg ccgttgtccg
    27421 tatgcttaac ccgttcaccc cgcacatctg cttcacgctg tggcaggaac tgaaaggcga
    27481 aggcgatatc gacaacgcgc cgtggccggt tgctgacgaa aaagcgatgg tggaagactc
    27541 cacgctggtc gtggtgcagg ttaacggtaa agtccgtgcc aaaatcaccg ttctggtgga
    27601 cgcaacggaa gaacaggttc gcgaacgtgc tggccaggaa catctggtag caaaatatct
    27661 tgatggcgtt accgtgcgta aagtgattta cgtaccaggt aaactcctca atctggtcgt
    27721 tggctaagcg cgggaggaag cgtgcgatat ctggcaacat tgttgttatc tctggcggtg
    27781 ttaatcaccg ccgggtgtgg ctggcatctg cgtgatacca cgcaggttcc ttccactatg
    27841 aaggtcatga tcctggactc aggcgatccg aacgggccat taagccgtgc agtgcgtaac
    27901 cagttacgtc tgaatggtgt cgagttgctt gataaagaaa ccacgcgtaa ggacgttcca
    27961 tccctgcgtt tgggtaaagt gagcatcgcg aaagataccg catcggtatt ccgtaacggt
    28021 caaacagcag agtatcagat gatcatgacg gtgaatgcga ccgtgttgat ccccggccgt
    28081 gatatctacc cgattagcgc caaagtcttc cgttcgttct ttgataaccc gcaaatggcg
    28141 ttagcgaaag ataacgaaca agacatgatc gtaaaagaga tgtacgaccg tgctgccgaa
    28201 cagctgattc gtaagctgcc aagcatccgt gctgcggata ttcgttccga cgaagaacag
    28261 acgtcgacca caacggatac tccggcaacg cctgcacgcg tctccaccac gctgggtaac
    28321 tgatgattcg gttgtacccg gaacaactcc gcgcgcagct caatgaaggg ctgcgcgcgg
    28381 cgtatctttt acttggtaac gatcctctgt tattgcagga aagccaggac gctgttcgtc
    28441 aggtagctgc ggcacaagga ttcgaagaac accacacttt ttccattgat cccaacactg
    28501 actggaatgc gatcttttcg ttatgccagg ctatgagtct gtttgccagt cgacaaacgc
    28561 tattgctgtt gttaccagaa aacggaccga atgcggcgat caatgaacaa cttctcacac
    28621 tcaccggact tctgcatgac gacctgctgt tgatcgtccg cggtaataaa ttaagcaaag
    28681 cgcaagaaaa tgccgcctgg tttactgctc ttgcgaatcg cagcgtgcag gtgacctgtc
    28741 agacaccgga gcaggctcag cttccccgct gggttactgc gcgcgcaaaa cagctcaact
    28801 tagaactgga tgacgcggca aatcaggtgc tctgctactg ttatgaaggt aacctgctgg
    28861 cgctggctca ggcactggag cgtttatcgc tgctctggcc agacggcaaa ttgacattac
    28921 cgcgcgttga acaggcggtg aatgatgccg cgcatttcac cccttttcat tgggttgatg
    28981 ctttgttgat gggaaaaagt aagcgcgcat tgcatattct tcagcaactg cgtctggaag
    29041 gcagcgaacc ggttattttg ttgcgcacat tacaacgtga actgttgtta ctggttaacc
    29101 tgaaacgcca gtctgcccat acgccactgc gtgcgttgtt tgataagcat cgggtatggc
    29161 agaaccgccg gggcatgatg ggcgaggcgt taaatcgctt aagtcagacg cagttacgtc
    29221 aggccgtgca actcctgaca cgaacggaac tcaccctcaa acaagattac ggtcagtcag
    29281 tgtgggcaga gctgaaaggg ttatctcttc tgttgtgcca taaacccctg gcggacgtat
    29341 ttatcgacgg ttgatatgaa atctttacag gctctgtttg gcggcacctt tgatccggtg
    29401 cactatggtc atctaaaacc cgtggaaacg ctggcgaatt tgattggtct gacgcgggtc
    29461 acaatcatcc ctaataatgt tcctccgcat cgtccccagc cggaagcgaa cagcgtgcag
    29521 cgtaaacaca tgcttgaact ggcgattgcc gacaagccat tatttactct tgatgaacgc
    29581 gagctaaagc gcaatgcccc ctcttacact gcgcaaacac tgaaagagtg gcggcaggaa
    29641 caaggaccgg acgtgccgct ggcgtttatt attggtcagg attcactgct gacctttccg
    29701 acctggtacg aatacgaaac gatactcgac aatgcacatt tgatcgtctg tcggcgtcca
    29761 ggttacccac ttgaaatggc gcaaccgcaa taccagcaat ggctggaaga tcatttgaca
    29821 cataacccgg aagatcttca ccttcagcct gccggtaaaa tttatctggc tgaaacgccg
    29881 tggtttaaca tctcggcgac catcatccgc gaacgtttgc aaaacggtga atcgtgtgag
    29941 gatttattgc cggaaccggt actgacttac attaaccaac aaggcttgta tcgctgatac
    30001 ctgtcgttac ggagggcatg atgcgactgt ggttaattcg tcatggtgaa acgcaagcga
    30061 atatcgatgg tctttacagc ggtcatgcgc ccacccccct gaccgcgcgc ggtattgagc
    30121 aagcgcaaaa tctgcatacg ctgctacatg gtgtttcctt tgatctggtt ttatgcagtg
    30181 aactggaacg ggcacagcat accgcacgac tggttctcag tgaccgccag ctccccgtgc
    30241 aaatcatccc tgaactcaac gaaatgtttt ttggcgactg ggagatgcga catcatcgcg
    30301 acctcatgca agaagatgcc gaaaactata gcgcgtggtg caatgactgg cagcatgcaa
    30361 tccccacgaa cggtgaagga tttcaggcat tttcgcaacg tgtggaacgc tttatcgcaa
    30421 ggcttagtga atttcagcac tatcagaata ttttagtcgt cagccatcag ggtgtactga
    30481 gtctgttaat cgcccgttta attggcatgc ctgccgaggc tatgtggcat tttcgcgttg
    30541 actaggggtg ttggagcgcc attgatatca accaaaaatt cgcgacgcta cgcgtcctca
    30601 atagccgtgc catcggggtc gagaatgcat gacttttctg tttttttacg ggtaagccgc
    30661 aacgaccatt gacagacccg ggcaggctga tattctccgc agccagactt ttttcgccag
    30721 acacgacttt gtagaaattg ttttacaaaa atggcgatgc aatctgcggc gcggggtggg
    30781 atgatagccc actttcgaaa gccgattcgg cgacaattgt cccgaaatcg cctctggttc
    30841 aggtatactg acagaccatt tttatctatt tgattcaccc agggggaaaa cttgcagggt
    30901 aaagcactcc aggattttgt tatcgacaaa attgatgacc tcaaaggtca ggacatcatc
    30961 gccttagacg ttcaaggcaa atccagcatc accgactgca tgatcatctg tacgggtacg
    31021 tccagccgtc atgttatgtc cattgctgac cacgttgtgc aggagtctcg cgcagcgggc
    31081 ctgttaccgc tcggcgtaga aggtgaaaac agcgccgact ggattgtcgt ggatttgggc
    31141 gatgtgattg tccatgtcat gcaggaagag agccgtcgcc tgtatgaact ggaaaaactc
    31201 tggagttaat gcgtgaagct gcaacttgtc gccgtgggaa cgaaaatgcc ggactgggta
    31261 caaaccggtt ttaccgagta cctgcgtcgt tttccgaaag atatgccctt cgagctgatt
    31321 gaaattccgg ccggaaaacg cggcaagaat gcggacatca agcgcatact cgacaaagag
    31381 ggtgagcaga tgttggcggc cgcaggcaaa aaccgcattg tcaccctcga tattccaggc
    31441 aagccctggg atacgccgca gttagccgct gagctggaac gctggaagct ggatggtcgc
    31501 gacgtcagtc tactgattgg cgggcctgaa gggttgtcgc ctgcctgtaa agcggcggcg
    31561 gagcagagct ggtcgctgtc ggcgcttacc ctcccccatc cgctggttcg cgtgctggtc
    31621 gcagagagtc tgtaccgggc gtggagcatc accaccaacc atccttatca ccgtgagtga
    31681 taagggagct ttgagtagaa aacgcagcgg atgaaactac agaactcttt tcgcgactat
    31741 acggcagagt ccgcgctgtt tgtgcgccgg gcgctggtcg cctttttggg gattttgctg
    31801 ctgaccggcg tgcttatcgc caacctgtat aatctgcaaa ttgttcgctt taccgactac
    31861 cagactcgct ctaatgaaaa ccgcattaag ctggtgccta tcgcgcccag ccgcggcatt
    31921 atctacgacc gtaatggtat ccctctggcc ctcaaccgca ctatctacca gatagaaatg
    31981 atgccagaga aagtcgataa cgtgcagcaa acgctggacg ctttgcgcag cgtggttgat
    32041 ctgaccgatg acgatattgc tgcattccga aaagagcgcg cacgttcaca ccgtttcacc
    32101 tctattccgg tgaaaactaa cctgaccgaa gtacaagtag ctcgctttgc cgtcaatcag
    32161 taccgttttc cgggtgtcga agttaaaggc tataaacgtc gttactatcc ttacggttcg
    32221 gcgttgaccc acgtcatcgg ctatgtgtcg aaaatcaacg ataaagacgt cgaacgcctg
    32281 aataatgacg gcaaactggc caactatgcg gcaacgcatg atatcggtaa gctgggcatt
    32341 gagcgttact atgaagacgt gctgcacggt cagaccggtt atgaagaggt tgaagttaac
    32401 aaccgtgggc gtgttatccg ccagttaaaa gaagtaccac cgcaagccgg acacgatatt
    32461 tacctgacgc tggatctcaa actccagcaa tatattgaaa cgctgctggc gggtagccgc
    32521 gcagctgtgg ttgtcaccga tccgcgtaca ggtggggtgc tggcgctggt ttccacgcct
    32581 agttatgacc caaacctgtt tgttgacggt atctccagca aagattattc cgccttgttg
    32641 aacgccccga atacaccgct ggtgaaccgc gccacacagg gggtttatcc tcccgcgtct
    32701 acagttaaac cctatgtggc ggtttcggca ttgagcgccg gggtgatcac gcgcaatacg
    32761 acgctgtttg acccaggctg gtggcaactg ccaggttcgg aaaaacgtta tcgtgactgg
    32821 aaaaaatggg gccacgggcg tctgaatgtc acaagatcgc tggaagaatc tgcggatacc
    32881 ttcttctatc aggtggccta cgatatgggg atcgatcgcc tctccgaatg gatgggtaaa
    32941 ttcggttatg gtcattacac cggtatcgac ctggcggaag aacgttccgg caacatgcct
    33001 acccgcgaat ggaaacagaa acgctttaaa aaaccgtggt atcagggtga caccattccg
    33061 gttggtatcg gtcagggtta ctggacagcg accccaatcc agatgagtaa ggcactgatg
    33121 atcctgatta atgacggtat cgtgaaggtt cctcatttgc tgatgagcac cgccgaagac
    33181 ggcaaacagg tgccatgggt acagccgcat gaaccgcccg tcggcgatat tcattccggt
    33241 tactgggagc tggcgaaaga cggtatgtac ggtgttgcta accgccctaa cggtacggcg
    33301 cataaatact ttgccagcgc accgtacaaa attgcggcga aatccggtac cgctcaggtc
    33361 ttcggtctga aagcgaacga aacctataat gcgcacaaaa ttgccgagcg tttacgtgac
    33421 cacaaactga tgaccgcctt tgcgccatac aacaatccgc aagtggctgt cgccatgatt
    33481 ctggagaacg gtggtgcggg tccggcggtt ggtacactga tgcgccagat cctcgaccac
    33541 attatgctgg gtgataacaa caccgatctg cctgcggaaa atccagcggt tgccgcagcg
    33601 gaggaccatt aatcatgacg gataatccga ataaaaaaac attctgggat aaagtccatc
    33661 tcgatcccac aatgctgctg atcttactgg cattgctggt ttacagcgcc ctggttatct
    33721 ggagcgccag cggtcaggat attggcatga tggagcgtaa aatcggccaa atcgcgatgg
    33781 gtctagtcat catggtggtg atggcgcaaa ttcctccacg cgtttatgaa ggctgggccc
    33841 cctatctcta tatcatctgt attattttgc tggtggcggt agatgctttc ggtgccatct
    33901 ctaaaggtgc tcaacgctgg ctggacctcg gtattgttcg ttttcagccg tcggaaattg
    33961 ccaaaatagc cgtaccactg atggttgcgc gctttatcaa ccgcgacgtt tgcccgccat
    34021 cgttgaagaa cactggcatc gcgctggtgc tgatatttat gcccacgctg ctggtggctg
    34081 cacagcctga cctgggaaca tcaatcctcg ttgcgctttc cggtctgttt gtactgttcc
    34141 tctctggcct tagctggcgt ctgattggcg tcgcagtagt gctggtggcg gcgttcattc
    34201 cgattctgtg gttcttcctg atgcatgatt accagcgcca gcgcgtaatg atgctcctag
    34261 acccggaatc agacccactc ggcgcgggct atcacattat tcagtctaaa attgctattg
    34321 gctccggcgg attacgcggc aaaggctggc tgcacggcac tcagtcacag cttgaatttc
    34381 tccccgaacg ccataccgac tttatcttcg cggtactggc ggaagagctg ggattagtgg
    34441 gcattctgat tctgctcgct ctctacattc tgctgatcat gcgcgggctg tggatagccg
    34501 ccagagcgca aaccaccttt ggtcgcgtca tggctggtgg cttaatgctg atattattcg
    34561 tttatgtctt cgtaaatatt ggtatggtaa gcggtattct gccggttgta ggggttccgc
    34621 tcccactggt cagttatgga ggatcggcgc taattgtgct gatggctggg ttcgggattg
    34681 taatgtcaat ccacacccac aggaaaatgt tgtcgaaaag cgtgtaagag gtgcgcaatg
    34741 cgtaagcagt ggctcgggat ctgcatcgcg gcaggaatgc tcgcggcatg tacaagcgat
    34801 gatggtcagc aacagacggt aagtgtaccg cagcctgcgg tatgtaacgg ccccatagtt
    34861 gaaattagcg gggcggaccc gcgtttcgaa ccactgaacg cgacggcaaa tcaggattac
    34921 cagcgcgacg gtaaaagcta caaaatcgtg caggatccgt ctcgatttag ccaggcggga
    34981 ctggcggcaa tctatgatgc cgaacccggc agtaacctga cggcctctgg cgaagctttc
    35041 gatccgacac agctgacggc ggcccatcca acgcttccga tccccagcta cgccagaatc
    35101 actaacctgg ctaacgggcg aatgatcgtg gtgcgcatta atgatcgcgg cccttacggc
    35161 aacgaccgcg ttatttcact ttctcgcgcg gcagctgacc gtcttaacac gtcaaacaac
    35221 accaaagttc gtatcgatcc gattattgtc gcccaggatg gttcgctttc tggtcctggt
    35281 atggcttgta ccacagtcgc caaacagact tacgccctgc cagcacctcc cgatttaagc
    35341 ggtggcgcgg gaacaagttc agtgtctggc ccgcagggtg acattcttcc ggtcagtaat
    35401 tcgacgctaa aaagcgaaga tccgaccggc gcgccggtaa ccagtagcgg tttcctcggc
    35461 gctccaacga ccttagcgcc tggtgtactg gaaggcagcg aaccgacgcc tgcgccacag
    35521 cccgttgtta cagctccgtc gacaacgcct gcaacctcgc ctgcaatggt gacaccgcaa
    35581 gccgcctcgc aaagcgccag cggcaacttt atggtgcaag tcggggccgt aagcgatcag
    35641 gctcgtgcgc aacagtacca acagcaactg ggacagaagt tcggcgtccc cggtcgcgta
    35701 actcaaaatg gcgcggtctg gcggatccag cttggcccat tcgccagcaa agccgaagcc
    35761 agtaccttgc agcaacgttt gcaaaccgaa gcccaattac agtcatttat tactaccgcg
    35821 cagtagcgta aagcaggcat ctgatgtgtc aatttctgta aatgacgtta acaaagtcac
    35881 gcggaaagtc agatgcctgc cggtagtgca tttgctatag tagggcactt ttttaattcc
    35941 atcacggatg tcgtagttca gaccatgaat accatttttt ccgctcgtat catgaagcgc
    36001 ctggcgctca ccacggctct ttgcacagcc tttatctctg ctgcacatgc cgatgacctg
    36061 aatatcaaaa ctatgatccc gggtgtaccg cagatcgatg cggagtccta catcctgatt
    36121 gactataact ccggcaaagt gctcgccgaa cagaacgcag atgtccgccg cgatcctgcc
    36181 agcctgacca aaatgatgac cagttacgtt atcggccagg caatgaaagc cggtaaattt
    36241 aaagaaactg atttagtcac tatcggcaac gacgcatggg ccaccggtaa cccggtgttt
    36301 aaaggttctt cgctgatgtt cctcaaaccg ggcatgcagg ttccggtttc tcagctgatc
    36361 cgcggtatta acctgcaatc gggtaacgat gcttgtgtcg ccatggccga ttttgccgct
    36421 ggtagccagg acgcttttgt tggcttgatg aacagctacg ttaacgcact gggcctgaaa
    36481 aatacccact tccagacggt acatggtctg gatgctgatg gtcagtacag ctccgcgcga
    36541 gatatggcgc tgatcggcca ggcattgatc cgtgacgtac cgaatgaata ctcgatctat
    36601 aaagaaaaag aatttacgtt taacggtatt cgccagctga accgtaacgg cctgttatgg
    36661 gataacagcc tgaatgtcga cggcatcaaa accggacaca ctgacaaagc aggttacaac
    36721 cttgttgctt ctgcgactga aggccagatg cgcttgattt ctgcggtgat gggcggacgt
    36781 acttttaaag gccgtgaagc cgaaagtaaa aaactgctaa cctggggctt ccgtttcttc
    36841 gaaaccgtta acccactgaa agtaggtaaa gagttcgcct ctgaaccggt ttggtttggt
    36901 gattctgatc gcgcttcgtt aggggttgat aaagacgtgt acctgaccat tccacgtggt
    36961 cgcatgaaag atctgaaagc cagctatgtg ctgaacagca gtgaattgca tgcgccgctg
    37021 caaaagaatc aggtcgtcgg aactatcaac ttccagcttg atggcaaaac gatcgagcaa
    37081 cgcccgctgg ttgtgttgca agaaatcccg gaaggtaact tcttcggcaa aatcattgat
    37141 tacattaaat taatgttcca tcactggttt ggttaaaaat tgaacacttg aaagtgtaat
    37201 ttccgtcccc atatactaag catcagtaaa aaactcccgc cttctggcgg gagttgctat
    37261 ttaattacgt tacgccggag ctgacatgaa aaccaaactt aacgaactgc ttgaattccc
    37321 tactcctttt acttacaaag ttatggggca ggcgttacct gagctggttg atcaggtggt
    37381 tgaagtggta cagcgccatg cgccaggtga ctacacccca acggtaaaac caagcagcaa
    37441 aggcaactac cactcggtat ctatcactat caacgccact catatcgagc aggttgaaac
    37501 actgtatgaa gaactaggca aaatcgatat tgtccgcatg gttctgtaac tcgcttctcc
    37561 gttacccggc ccctggtcgg gtaactcccc tctcctcgct gtgatatact tttcccccac
    37621 ttttactcat tctccacgga gatgcagttt tgtatcagga taaaattctt gtccgccagc
    37681 tcggtcttca gccttacgag ccaatctccc aggctatgca tgaattcacc gatacccgcg
    37741 atgatagtac ccttgatgaa atctggctgg tcgagcacta tccggtattc acccaaggtc
    37801 aggcaggaaa agcggagcac attttaatgc cgggtgatat tccggtgatc cagagcgatc
    37861 gcggtgggca ggtgacttat cacgggccgg ggcaacaggt gatgtatgtg ttgcttaacc
    37921 tgaaacgccg taaactcggt gtgcgtgaac tggtgacctt gcttgagcaa acagtggtga
    37981 ataccctggc tgaactgggt atagaagcgc atcctcgggc tgacgcgcct ggtgtctatg
    38041 tcggggaaaa gaaaatttgc tcactgggtt tacgtattcg acgcggttgt tcattccacg
    38101 gtctggcatt aaacgtcaat atggatcttt caccattttt acgtattaat ccttgtgggt
    38161 atgccggaat ggaaatggct aaaatatcac aatggaaacc cgaagcgacg actaataata
    38221 ttgcaccacg tttactggaa aatattttag cgctactaaa caatcctgac ttcgaatata
    38281 ttaccgctta attgcatacc tcaatggccc aatttacact gggtcattac tcataatacc
    38341 tgctaatgct ttacatctcc ttcgcgtttg tatactttct ttatcacttg cagaaatgcg
    38401 gtgctacgcc ttgctccgta actcacccca ttgattaacc tattgaaata gttgattatg
    38461 gtgagcaaaa gcgttccgtt ccaattagac ataagattat tgtaattttt ttaaatatta
    38521 tttttccatg aataaaattg gagtaagtgc gtggatagta ataatcaaat tgaaccctgt
    38581 ttaagcagaa agtcgtcaga aggtaaacca caaatattta caaccttacg aaatattgat
    38641 cttaaccttc tgactatttt tgaagctgta tatgtacata aagggatcgt taatgcagcg
    38701 aaagtgctta atctgacccc ctcggcaatc agtcagtcta ttcagaaact gcgcgttata
    38761 ttccctgacc cattgtttat tcgcaaaggc cagggtgtca ctcctaccgc atttgcgatg
    38821 catctacatg agtatatcag tcagggcctt gagtccattc ttggcgcgct ggatatcgaa
    38881 ggaagctatg ataagcaacg aacgataact attgcgacca ctccctcggt cggagccctg
    38941 gtccttcctg tcatctatcg ggcgattaaa actcactatc cgcagctttt actgcgcaac
    39001 ccgcccatca gcgacgcgga aaaccaactc agtcagtttc aaaccgatct catcatcgat
    39061 aacatgtttt gcaccaatcg tacggtgcaa catcatgttc tgttcaccga caatatggtg
    39121 ttaatttgcc gcgagggaaa tccactactc tctttagaag atgacagaga gactatcgac
    39181 aacgctgcgc atgtactcct gttaccggaa gggcaaaatt tcagcggtct gcggcagaga
    39241 gttcaagaga tgtttccgga ccggcaaatc aatttcacca gctacaacat tttgacaatc
    39301 gctgcactgg ttgccaacag tgacatgtta gcgattattc ccagccgttt ttataacctg
    39361 tttagtcgtt gctggccgct ggaaaaactg ccttttccgt ccttaaatga ggagcaaata
    39421 gatttctcta tccactacaa caaattcagc ctgcgtgatc ccatcctgca cggggtaata
    39481 gatgttatcc gtaatgcatt ttgaaaaagt attgttacag ccagatcaca gcgcagaacg
    39541 ataaaatggc acaatccaac aacaatttta cattttcgcg accgctttgg ctgctttcag
    39601 gtccgtttca atgatatact gccagtcgtt aattcaaaaa tagttgataa ttacaacaat
    39661 ctattgaatt gaaacgcttt ccttcgtaat tcgcaactgg aacacgcacg ctatgagtaa
    39721 acccattgtg atggaacgcg gtgttaaata ccgcgatgcc gataagatgg cccttatccc
    39781 ggttaaaaac gtggcaacag agcgcgaagc cctgctgcgc aagccggaat ggatgaaaat
    39841 caagcttcca gcggactcta cacgtatcca gggcatcaaa gccgcaatgc gcaaaaatgg
    39901 cctgcattct gtctgcgagg aagcctcctg ccctaacctg gcggaatgct tcaaccacgg
    39961 cacagcaacg tttatgatcc tcggcgctat ttgtacccgc cgttgtccgt tctgtgatgt
    40021 tgcccacggt cgcccggtag ctcctgatgc caatgaacca gtgaaactgg cgcagaccat
    40081 tgccgatatg gcgctgcgtt atgtggttat cacctccgtt gaccgtgatg acctgcgcga
    40141 tggcggtgcc cagcactttg cggattgcat tactgccatt cgggaaaaaa gcccgcaaat
    40201 caaaattgaa actctggtgc cggatttccg tggtcgtatg gatcgtgctc tggatattct
    40261 gactgcaacg ccaccagatg tgttcaacca taacctggaa aacgtaccgc gtatttaccg
    40321 tcaggtacgg cctggtgcag attacaactg gtcgctgaag ctgctggaac gctttaaaga
    40381 agcgcatccg gaaatcccga ccaagtctgg tctgatggtg ggactgggtg aaaccaatga
    40441 agaaattatt gaggtaatgc gcgacctgcg ccgtcatggt gtgacgatgt taacgctggg
    40501 gcaatatttg cagccaagcc gccatcacct gccggttcaa cgttacgtta gcccggatga
    40561 gttcgacgaa atgaaagccg aagcgctggc gatgggcttt acccatgctg catgcggtcc
    40621 gtttgtccgc tcttcttacc acgccgattt gcaggcgaaa gggatggaag ttaagtaatc
    40681 ctgtaataat tacttacatc tgtctgataa aaaacccgct ttttg
//
LOCUS       DAJVRF010000018        39801 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJVRF010000018 DAJVRF010000000
VERSION     DAJVRF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39801
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.018"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(15..90)
                     /locus_tag="OQN13_000890"
     tRNA            complement(15..90)
                     /locus_tag="OQN13_000890"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55..57),aa:Lys,seq:ttt)
     gene            complement(139..214)
                     /locus_tag="OQN13_000891"
     tRNA            complement(139..214)
                     /locus_tag="OQN13_000891"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(179..181),aa:Lys,seq:ttt)
     gene            complement(218..293)
                     /locus_tag="OQN13_000892"
     tRNA            complement(218..293)
                     /locus_tag="OQN13_000892"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(258..260),aa:Val,seq:tac)
     gene            complement(443..518)
                     /locus_tag="OQN13_000893"
     tRNA            complement(443..518)
                     /locus_tag="OQN13_000893"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(483..485),aa:Lys,seq:ttt)
     gene            complement(683..1474)
                     /gene="cpoB"
                     /locus_tag="OQN13_000894"
     CDS             complement(683..1474)
                     /gene="cpoB"
                     /locus_tag="OQN13_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCS1614322.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1484..2005)
                     /gene="pal"
                     /locus_tag="OQN13_000895"
     CDS             complement(1484..2005)
                     /gene="pal"
                     /locus_tag="OQN13_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCS1614323.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2040..3332)
                     /gene="tolB"
                     /locus_tag="OQN13_000896"
     CDS             complement(2040..3332)
                     /gene="tolB"
                     /locus_tag="OQN13_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCS1614324.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3465..4697)
                     /gene="tolA"
                     /locus_tag="OQN13_000897"
     CDS             complement(3465..4697)
                     /gene="tolA"
                     /locus_tag="OQN13_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCS1614325.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASSAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            complement(4762..5190)
                     /gene="tolR"
                     /locus_tag="OQN13_000898"
     CDS             complement(4762..5190)
                     /gene="tolR"
                     /locus_tag="OQN13_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCS1614326.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5194..5886)
                     /gene="tolQ"
                     /locus_tag="OQN13_000899"
     CDS             complement(5194..5886)
                     /gene="tolQ"
                     /locus_tag="OQN13_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCS1614327.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5883..6287)
                     /gene="ybgC"
                     /locus_tag="OQN13_000900"
     CDS             complement(5883..6287)
                     /gene="ybgC"
                     /locus_tag="OQN13_000900"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS1614328.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPCALPKSIVAEFKQ"
     gene            complement(6437..6730)
                     /gene="ybgE"
                     /locus_tag="OQN13_000901"
     CDS             complement(6437..6730)
                     /gene="ybgE"
                     /locus_tag="OQN13_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCS1614329.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6730..6843)
                     /gene="cydX"
                     /locus_tag="OQN13_000902"
     CDS             complement(6730..6843)
                     /gene="cydX"
                     /locus_tag="OQN13_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCS1614330.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6858..7997)
                     /gene="cydB"
                     /locus_tag="OQN13_000903"
     CDS             complement(6858..7997)
                     /gene="cydB"
                     /locus_tag="OQN13_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS1614331.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(8013..9581)
                     /gene="cydA"
                     /locus_tag="OQN13_000904"
     CDS             complement(8013..9581)
                     /gene="cydA"
                     /locus_tag="OQN13_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCS1614332.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10177..10404)
                     /locus_tag="OQN13_000905"
     CDS             complement(10177..10404)
                     /locus_tag="OQN13_000905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614333.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
     gene            complement(10429..13062)
                     /gene="mngB"
                     /locus_tag="OQN13_000906"
     CDS             complement(10429..13062)
                     /gene="mngB"
                     /locus_tag="OQN13_000906"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCS1614334.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLQDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGGVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(13080..14996)
                     /gene="mngA"
                     /locus_tag="OQN13_000907"
     CDS             complement(13080..14996)
                     /gene="mngA"
                     /locus_tag="OQN13_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCS1614335.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTTYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            15165..15887
                     /gene="mngR"
                     /locus_tag="OQN13_000908"
     CDS             15165..15887
                     /gene="mngR"
                     /locus_tag="OQN13_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCS1614336.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15991..16860)
                     /gene="sucD"
                     /locus_tag="OQN13_000909"
     CDS             complement(15991..16860)
                     /gene="sucD"
                     /locus_tag="OQN13_000909"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCS1614337.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16860..18026)
                     /gene="sucC"
                     /locus_tag="OQN13_000910"
     CDS             complement(16860..18026)
                     /gene="sucC"
                     /locus_tag="OQN13_000910"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCS1614338.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(18120..19337)
                     /gene="odhB"
                     /locus_tag="OQN13_000911"
     CDS             complement(18120..19337)
                     /gene="odhB"
                     /locus_tag="OQN13_000911"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCS1614339.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(19352..22153)
                     /gene="sucA"
                     /locus_tag="OQN13_000912"
     CDS             complement(19352..22153)
                     /gene="sucA"
                     /locus_tag="OQN13_000912"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCS1614340.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22454..23170)
                     /gene="sdhB"
                     /locus_tag="OQN13_000913"
     CDS             complement(22454..23170)
                     /gene="sdhB"
                     /locus_tag="OQN13_000913"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCS1614341.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYPLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(23186..24952)
                     /gene="sdhA"
                     /locus_tag="OQN13_000914"
     CDS             complement(23186..24952)
                     /gene="sdhA"
                     /locus_tag="OQN13_000914"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCS1614342.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLHRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24952..25299)
                     /gene="sdhD"
                     /locus_tag="OQN13_000915"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24952..25299)
                     /gene="sdhD"
                     /locus_tag="OQN13_000915"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCS1614343.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(25293..25697)
                     /gene="sdhC"
                     /locus_tag="OQN13_000916"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(25293..25697)
                     /gene="sdhC"
                     /locus_tag="OQN13_000916"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS1614344.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            26078..26185
                     /locus_tag="OQN13_000917"
     CDS             26078..26185
                     /locus_tag="OQN13_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614345.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26391..27674
                     /gene="gltA"
                     /locus_tag="OQN13_000918"
     CDS             26391..27674
                     /gene="gltA"
                     /locus_tag="OQN13_000918"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCS1614346.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            28064..28630
                     /locus_tag="OQN13_000919"
     CDS             28064..28630
                     /locus_tag="OQN13_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1614347.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28681..31139
                     /locus_tag="OQN13_000920"
                     /pseudo
     CDS             28681..31139
                     /locus_tag="OQN13_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            31154..31885
                     /locus_tag="OQN13_000921"
     CDS             31154..31885
                     /locus_tag="OQN13_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS1614348.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31882..32943
                     /locus_tag="OQN13_000922"
     CDS             31882..32943
                     /locus_tag="OQN13_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1614349.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            33095..34141
                     /locus_tag="OQN13_000923"
     CDS             33095..34141
                     /locus_tag="OQN13_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCS1614350.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(34138..34929)
                     /gene="nei"
                     /locus_tag="OQN13_000924"
     CDS             complement(34138..34929)
                     /gene="nei"
                     /locus_tag="OQN13_000924"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCS1614351.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGLVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34965..35699)
                     /gene="pxpA"
                     /locus_tag="OQN13_000925"
     CDS             complement(34965..35699)
                     /gene="pxpA"
                     /locus_tag="OQN13_000925"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCS1614352.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(35689..36621)
                     /gene="pxpC"
                     /locus_tag="OQN13_000926"
     CDS             complement(35689..36621)
                     /gene="pxpC"
                     /locus_tag="OQN13_000926"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCS1614353.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(36615..37271)
                     /gene="pxpB"
                     /locus_tag="OQN13_000927"
     CDS             complement(36615..37271)
                     /gene="pxpB"
                     /locus_tag="OQN13_000927"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCS1614354.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(37294..38037)
                     /gene="ybgI"
                     /locus_tag="OQN13_000928"
     CDS             complement(37294..38037)
                     /gene="ybgI"
                     /locus_tag="OQN13_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCS1614355.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            38308..39789
                     /gene="dtpD"
                     /locus_tag="OQN13_000929"
     CDS             38308..39789
                     /gene="dtpD"
                     /locus_tag="OQN13_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCS1614356.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         9643 a        10702 c         9690 g         9766 t
ORIGIN      
        1 ttaatcattc aggatggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt
       61 caactgctct accaactgag ctaacgaccc atacgggtgt tacctgaaag atttctactc
      121 ggggccaatc tttaagattg gtgggtcgtg caggattcga acctgcgacc aattgattaa
      181 aagtcaactg ctctaccaac tgagctaacg acccgagtgg tgggtgatga cgggatcgaa
      241 ccgccgaccc cctccttgta agggaggtgc tctcccagct gagctaatca cccgatactg
      301 cgctggatac tacaaactac ttccaccgaa cccaccgtaa ttagtggtgg gtcgtgcagg
      361 atgactcgct tcgctcgccc tacgggccgt tgctgacgca acgttatcct acacgcttaa
      421 catctgtctt cgatgctaaa actggtgggt cgtgcaggat tcgaacctgc gaccaattga
      481 ttaaaagtca actgctctac caactgagct aacgacccac tttttcgttg ctttcggttt
      541 gtttgatatc ccgtggcaac ggcggcatat attactgatt tcagacttga gcgcaacaaa
      601 aatttcgatg cagatcactc aactgcttat gattcgcacg acacgaccag aaataatgcg
      661 acttctggtc gtgtgttatg cattacatcg cgttcagacg tttttgtgcc tgtttagcgc
      721 catcagtacc agggtattta ctgataacct gctggtatac ggctttcgct tttgcggtgt
      781 cacctttgtc ctgcatgatg acgccgactt taaacatcgc atcagcagcc tttggtgact
      841 tcggatagtt tttcactacc gaagcaaaat agtacgccgc atcatctttt ttacccttgt
      901 tgtagtttaa ctgacccagc caataattgg cgtttggcag gtaagttgaa tcagggtaat
      961 ttttgatgaa attctgaaat gccaccattg cgtcatcctg gcgggattta tcctgcacca
     1021 gcgcaatagc tgcattgtaa tccgtgtttg catcgccgct tttcaccggc gcgccagcat
     1081 tcgcagtacc agcatcagct gtcggcgtcg ttgatgccgc cgcaccgctt tgatcgctgc
     1141 tggttgattg cgccgctgca ccaccgctgc tgaggctgtc gatctgcaac aggatctgct
     1201 tttgccgctc cacgacctga ttcagttgat actgattttc ctgaatctga ccacgcaggg
     1261 aatcaatatc ggattgatta tcagaaagtt gttgctggag ttgggttaaa agctggctgt
     1321 gagcattaga aatacgctca agttgagtga cgcggtcttc gaccgagcct gagccgacac
     1381 tactgattgg tgcctgagca aaagcggccc agggggccgc tataccaacc agtaacgaca
     1441 gactcaatag ttgatgtctg aagttactgc tcatgcaatt ctcttagtaa accagtaccg
     1501 cacgacggtt tttggagtat gccgcttcgt catgacccag tactgcaggt ttttctttac
     1561 cgtaagaaac gatggagatc tggtctgcag aaacgccttt accctgcagg tacatcttaa
     1621 cggcgttcgc acgacgttca cccagggaga tgttgtattc cggagtacca cgttcgtccg
     1681 cgtgaccttc tacggtgact ttgtaagacg ggttgctacg caggaagttt gcatgtgcat
     1741 ccagcatttg agcgaagtca gaacggatat cgtacttgtc cagatcgaag taaacgatgt
     1801 tgttctgctg cagctgttgc atttgcagac gagcctgctc ttcggaagac atgttgccgt
     1861 tgccgccgtt cgcatccata ccagtgccgg cacccagcat gccttcgctg ccgtcattgc
     1921 tggcgttctt gttggaagaa catgccgcaa ttgccataac aggcagagca atcatcagcc
     1981 ctttcagcac tttgttcagt tgcatttcaa tgattccttt actattcaat taattattat
     2041 cacagatacg gcgaccaggc agggaatttg acctgtccat cagttgccgg aagacgcgct
     2101 ttgaaacgcc catctgtaga aaccaaattc agcacggatc ccatcccctg agaagagctg
     2161 tagattacca tagtgccgtt aggtgccaga cttggcgttt catccaggaa cgtggacgac
     2221 agaacttgta cgcctcccgt tgccagatct tgtttggcaa tgtgctgctg cccaccgttg
     2281 gagctgacca ttaccataaa tttaccgtcg ctgctgacat ccgcatcctg gttctgcgaa
     2341 ccttcccagg taatacgttg tggcgcaccg ccgttgatat tcactttata aacctgtgga
     2401 cgaccggcct ggtcagaagt aaatgccagg ttctggctgt ccgggaacca ggtcggttcg
     2461 gtattgttac tgcgaccatc agtcacctgg cggatctgac cagaagccaa atccattacg
     2521 tacaggttca ggctaccggt tttcgacaag gcgaatgcca gtttgctgcc gtctggcgag
     2581 aatgcaggcg caccgttgtg acgcgggaat gaagccacct gacgtacagc gccgtttgcc
     2641 agcgtttgga taactagcgc ggaacgaccg ctttcgaagg tcacataagc cagtttagaa
     2701 ccgtctggtg accacgccgg agacatcagc ggctgtggtg aacggtgaac gacaaactgg
     2761 ttgtaaccgt catagtcaga tacgcgcagt tcatacggga actgaccgcc gttggtctga
     2821 acaacgtagg caatacgggt acggaacgca cctttaatgc cggtcagttt ttcaaacact
     2881 tcatcactgg cggtatgacc agcatagcgc agccactgct tgttcacttt gtacgagttc
     2941 tgagcaagta cagtacccgg tgcgccgcca gtgtcaacaa gttgataagc aacattgtaa
     3001 gaaccatccg gattcggagt gacctgaccg acaactacag cgtcaatacc cagcgcggac
     3061 catgcagctg gttgtacttc ctgcgcacta cccggctgct gtggcagacg agcgcgatct
     3121 aacggattaa atttaccgct gttacgcaag tcggcagcaa cgatgccgcc aatatcttca
     3181 ggtgccgcac cagggcccgc ccactggaaa ggaacaacac caataggacg accggaatct
     3241 acaccgctgt cgatcacaat gcggacttca gcatgcagaa ctgatgccca cagtatgaga
     3301 aaaccaaatg ctactcgtaa tgcctgcttc atcatatctc ccttatctgg accgatggcc
     3361 cacgataatt tagcagaatg ttaacaaact aaaatacaca aaactaccag aaccccgtgg
     3421 caaccggtgc ctgatgttga ccgtcggaac agtcaacatc gcgattacgg tttgaagtcc
     3481 aatggcgcgt ttttgaacac ttcatatact gcctggcttg gtggtttcgg gatcttcgca
     3541 agtttagctg ctgccaacgc agcctgacaa agtgcgggat cgccaccttc aggtttgata
     3601 tccagtaaca taccatcggg tgccagtttt atgcgcagcg tacaggtttt gcctgcatag
     3661 gacgatgcgt catagaactt actttcgata gcagatttaa tctgcccggc atagttattg
     3721 atatcggccc ctgatgcgcc attgttttta gtattaccac tcccggcaga cgaagcattg
     3781 ttccctttcg cccctccccc cgttttcggt gcattcttac cagagcttag ctcaccgaaa
     3841 atatcatctg cctctgcggc agcttttgct gcagcggctt tttcagctgc tgcttttgcc
     3901 gctgccgctt ttttgtctgc ggcggctttt tctgccgctg cttttgccgc tgccgctttt
     3961 ttgtctgcgg cggctttttc tgccgctgct ttcttatcag ctgctgcctt atcagcagcc
     4021 gctttttctg ctgctttttt gtcggctgca gctttctctg ctgccgcttt ctttcttgct
     4081 tcagctgcag cagcttctgc cgcttccgct ttcttcttca gtgccgcagc ggctgcctcg
     4141 gcttttttct gcgcttcggc tgcggctttg gcggcttctg cttctgcttt tttcttcgcg
     4201 tctgccgccg ctttcttcgc tgcttcttcc gcagctttag catccgcttc ggccttcgct
     4261 ttagcatctg ccgccgcttt cgctgccgcc tcttccgctt gcttctgctt taactcggcc
     4321 tgttttgcgg cttcttcagc ctgctttttc tgttcctgag cagctaaccg ctctttctca
     4381 agttgcttca ggcgttcctg ttcagccgct tgtttctcac gcagttcttc agcagcctgc
     4441 tgttccttta tcttgcgctg ctcatcagaa cgcttcgcgc ttgattcctg gctttgcatg
     4501 cgtttgtact gctcaactac cgcacctgaa tcaaccatga cagcgtcgat ggacgaacca
     4561 ccgccgcctc cggctgaagc ttctatattc tcatcgaacg aactccagag cagcgccgca
     4621 aataagatga catgcagcac tgctgaaata attatcgccc gcttgagctt gtcgttttgt
     4681 tcggttgcct ttgacactct cggtttccaa aaactgttcg cctgttaccc gctctctttc
     4741 aagcaaggga aacgcagatg tttagatagg ctgcgtcatt aaaccaaccg atttcacacc
     4801 cgcactatgt aacaagttca gtgctttaat tatttcatcg taaggcacat cttttgcgcc
     4861 accgatcaga aagaccgttt tcgggttggc cttgaaacgg ctggacactt ccgccaccac
     4921 ctgctctggt ggtaaacgct ccaggcgatc tttctcaacc accacggtgt actgaccaat
     4981 accagacact tcaacaatca ctggcggatt atcgttactg ctcaccgcct gtgattcagt
     5041 agcgtctggc agatcgacct ccacgctctg ggtgatgatg ggcgctgtcg ccataaagat
     5101 cagcaacagc accagcagta cgtccagcaa cggtacaatg ttgatttcgg acttgagatc
     5161 gcgacgacct cgtccacgcg ctctggccat ggcttacccc ttgttgctct cgctaacggt
     5221 aaacgcctgg cggtgcagaa tcgcggtaaa ctcttccata aagttgtcgt aattcagttc
     5281 cagtttgttt acgcgctggt tgaggcggtt ataagccatt accgccggaa ttgcggcgaa
     5341 cagaccaatt gcagtcgcaa tcaacgcttc tgcgataccg ggcgcaacca tttgcagtgt
     5401 tgcttgtttt accgccccga gggcgataaa ggcgtgcatg atcccccaga ccgtaccaaa
     5461 cagaccaata tacgggctga tggagccaac cgtaccgagg aacggaatgt gcgtttccag
     5521 attttcaagt tcacggttca tggagatacg catagcacgc gacgcccctt ccactacggc
     5581 ttccggcgca tggctattgg cacgatgcag gcgcacaaac tctttgaacc cgctgtaaaa
     5641 tatttgttcc gaaccagtca gattatcccg tttcccctga ctctcttgat agaggcgaga
     5701 gagttcgatt ccagaccaga atttatcttc aaacgcttcg gcttcgcgcg ccgctgcgtt
     5761 aagaatacgg gtccgctgga taataatggc ccaggatgcg attgaaaaac caatcaaaat
     5821 caacatgata agtttaacca gaaggctagc cttcaggaac aaatcaagga tattcatgtc
     5881 agtcactgct taaactccgc gacaatagac ttgggaagcg cacaaggctt cattttgagt
     5941 gggtcaacgc aaacaaccag aacctctgct tcattcagca aagtattctc ggcgttgaca
     6001 atacgttgcg tgaaaaccaa agaggtgcca cgcattgatg ttatttcagt ctgtatttcg
     6061 agcatatcgt cgagccgcgc aggtgcgtaa tattccaccg tcattttacg taccacaaag
     6121 gcaacgcgtt cagccatcag cgcctgctga ctgaagtgat gatgacgcag catctctgtg
     6181 cgtgctcttt cataaaaagc gacgtaactg gcgtggtaca ccacaccacc ggcatcggta
     6241 tcttcatagt agacgcgaac cggccatcga aacagcgttg tattcacttt acatcccggt
     6301 aatgcaacaa aagttagagc ttttaaacgc tgctactata cgcgcgcgat tgaggtttgg
     6361 gaatgagggc aagttaaggg agcgtaaaaa tttatgggcc tggagcggcc cataattttt
     6421 taaagaaaat tctgatttaa aagaagaaga aaatcagccc gacaatgaga acaatatcgg
     6481 caagcaatgg gcaaaaaatc ccttgccaaa gaaccttctg cggacgaaag cccacgccgt
     6541 gaatcacacc agcacagacg gcccacatca gcaataaacc atgccagatt tccagttcac
     6601 tggtcttcgc ggcaaaacga gatgggtccc aaaacataca tcctgctaac agaagcgcca
     6661 tcacgaagga aagcgcccgc agggggcgct tgtccattac cgcatacaag gtcgcgataa
     6721 tcttactcat cagatgtctt cttgaccggc tttgcctgat tcgacgtgtt caagcgccag
     6781 cgcggtgatt accccaaacg aacaggcaag aagcgttccc agaatccatg cgaaatacca
     6841 catttttagc tccttactta gtacagagag tgggtgttac gttcaatatc ttctttggtg
     6901 atacgaccga acattttcca gtaacaccaa gcggtgtaga gcagaatgat cggtaccaga
     6961 accaccgcaa cccaggtcat gacgttaagc gtcagctggc tggaagttgc atcccacatt
     7021 gtcagacttg cgttcatcat ggtgctggac ggcatcacaa acgggaacat tgcgataccg
     7081 gctgtcagga tgatgcaggc cagcgtcagg gaggagaaca caaacgccca cgcggcttta
     7141 tccatacgtg cagtcaggat ggtcagcagc ggaagaacca cacccagtgc cggaatagcc
     7201 cacagaattg gcgtgttgtt gaagttaacc agccatgcgc cagcttcacg aaccacttct
     7261 ttattcagtg ggttagaggc tgcgtaatgg tccattgtcg atttcacgac ataaccatcg
     7321 ataccgtaca tcacccatac gccagccagt gcgaaacaga ccagtgtcac cagcgcagcc
     7381 acctgagccg ttgcacgggt acgcaggtgc agttcgccca cggtacgcat ttgcagatag
     7441 gttgcgccct gagtaatgat catccctacg ctcaccacgc ctgccagcag gccgaacggg
     7501 ttaagcaact ggaagaagtt accggtgtag tacagacgca gatattcatc aacgttgaac
     7561 ggtacgccct gcaacaggtt accgaacgct acaccaatta ccagcggcgg aacgaaacta
     7621 ccaatgaaga tgccccagtc ccacatgtta cgccagcggg tttcttcaat cttggagcgg
     7681 tagtcaaaac cgaccggacg gaagaacaaa gacgccagca cgaggatcat cgccacatag
     7741 aagccggaga acgcagcggc atagaccatc ggccaggcag caaagagtgc gccgcccgcg
     7801 gtgatcagcc aaacctggtt accgtcccag tgtggtgcaa tggagttaat cataattcga
     7861 cgctcggtgt cgttacgacc gaggaaacgg gtgagcatgc ccacccccat gtcgaaaccg
     7921 tcagtgactg caaaaccaat cagcagaacg ccaaccagca gccaccagat aaaacgcaat
     7981 acttcataat cgatcatttg acgactcctg tcttagcgtg ccggctgagt agtcgtggaa
     8041 gactgctcaa agtgatagcg accggttttc aggctgcttg ggccgaggcg tgcaaacttg
     8101 aacattaaga acaattctgc caccaggaac agggtataca ggccgcaaat cagcaccatt
     8161 gagaagatga gatcgcctgc ggtcagtgac gagttcgcca cagctgtcgg cagcacttca
     8221 ccgatagccc acggttggcg gccatattca gccacgaacc agcccgcttc tacagcaatc
     8281 cacggcagcg gaataccgta cagcgcggcg cgcagaagcc attttttctc gccaatgcgg
     8341 ttgcggatga cactccagaa agagagcgcg atgattgcca gaagcaggaa gccacacgcc
     8401 accatgatac ggaacgcaaa gtacagcggc gctacacgcg ggatggagtc tttggttgcc
     8461 tgttgaatct gcgcttcagt cgcatcagcc acgtttggcg tatagcgttt cagcagcaga
     8521 ccgtaaccga ggtctttctt catgctattg aactggtcac gaaccgcctg gtcggtagaa
     8581 ccagaacgca gttgttcgag cagagagtac gccttcatcc cgttacgaat gcgttcttca
     8641 tgctgcacca tcagctcttt caggccgata accggggtat ccacggaacg cgttgcaatg
     8701 atgcccagtg cgtaagggat ctgaatcgca aatttgttcg tctcctcttc ctgatcagga
     8761 atgccgaaca gagtaaaggc agcaggcgca ggttgcgttt cccactcggc ttcaatagca
     8821 gccagtttgg ttttctgcac gtcgcccatt tcgtagccgg attcatcacc cagaacaata
     8881 acagacagaa cagcagccat accgaagctg gcagcgatag caaaggagcg tttagcgaag
     8941 gcgaagtcac gacctttcag catataccat gcgctgatac cgaggatgaa catcgcgcca
     9001 gtcacataac cagacgctac agtgtgaacg aatttcacct gagcaaccgg gttaagcacc
     9061 agctcggaga agctcaccat ctccatacgc atagtttcaa agttgaaatc ggacgcgatt
     9121 gggttttgca tccagccgtt cgcaaccaga atccacagtg cggacaggtt tgaaccgagc
     9181 gccaccagcc aggtgacaca catatgctga actttaccca gacgatccca accgaagaag
     9241 aacagaccta caaaggtgga ttcgaggaag aaggccatca gaccttcgat tgccagcggc
     9301 gcaccgaaga tatcccctac atagtgggaa tagtaagacc agttagtccc gaactggaac
     9361 tccatggtca gaccggtagc cacacccaga gcgaagttga taccaaacaa cttgccccag
     9421 aacttggtca tatctttata aatctgtttg ccggagagga cgtagaccgt ttccataatg
     9481 gccagcagga acgccatacc gagcgtcagt ggcacaaaaa ggaagtggta catcgcggtc
     9541 aaggcaaact gtaagcgcga cagttcgact atatctaaca tcatgactcc ttgctcatcg
     9601 catgaagact ccgagagtga accccgttag aaagggtcac acgcatgccc caatagaaat
     9661 ttgtttgaac actttcatca taccatgtga atgcacacaa aaacggcgta aagaaaggtt
     9721 gcaaacatgt taataaaaac tcaaattgat cccacgtata tattacgctg caaaatcctt
     9781 acaataaaca ggtttttgtt gaagcaaatt tgcatttttc gacggtgatc aatttatagc
     9841 taaattaccg cctttcagcc aatttgatcg agaacaattt atctcttttt gatgccaatt
     9901 tccaagactt atacattgat aaatatcaat tcctacaaat tatgttaatc atgtttcaat
     9961 aaggtaactt tagtgacatt tatgtttaaa atgtgtgagt tataaggctg aaattagttt
    10021 cggttggcaa tttaaagata aaaagagacg aagcaaaaac agcacggttg accttaattc
    10081 tttttttggt cactggctaa tacgacgcag agtttcagga ttactaataa aggcataaat
    10141 ccaccccgcc gatattaaca tttaattaac ttgtaattat attataatta actcacccag
    10201 ggttattatt ttgttaaaaa taaagccaca tatacgtggc ttgagagtga agataaacgc
    10261 aaaattacct gatgcgctac gcttgccagt gtctacatca tctctgcaat atattgaatt
    10321 tgcctgcttt tgtagaccga ataaagcgtt catgccgtat ccggcatgaa caaagcgccc
    10381 tttgtcaaca atctggccgc gcatatgtgg cttcggtttg attgctattc aggcaagccg
    10441 gtaactgaac gtccgtgact ggccgggtaa aaaaggcccc gatagatttg aaccttgatt
    10501 ttcctcggtg gtaatgtgtt catccatcat cgtttctgag caagaaatca cctcgcgact
    10561 gaaagcaaca gtcgcatcac aggttgctga ttcagccgga ttaaacagcc gtaaaatcac
    10621 ttcttgtcgg tcttcagctt tcttaagtgc gcttatcagg catcccactg ggggcatttt
    10681 caacaaacta taactttccg gcacgttgaa tccggctttg ttgagcttca tcgcatccca
    10741 tgggattttg ttgtaacact gtactggagt cagccatgct cgcgcctgct gagctacacc
    10801 agcggcggtt ggcgtaccgg tataactcaa taaacttagg cgacaagaaa gcagaccacg
    10861 tagttgtgag tccgggactg gcattttaat tcccgaaggc cgcccaggcc ttaaaagcag
    10921 atcttctttg cccagtaagc ccacgccacg cagcaacgta atggcaaagg ttttcttctc
    10981 ttcaccgatg acttcaaatt cacgtaaccc ttcgctaaag acagccatgc cgttacgccc
    11041 ttcctgtaag gcaacatagt tgagcatatt ccataccgga accggcgctt ctttccagcc
    11101 ttcttgctgc cagttgttca ttgcactgtc gttcacgggg cgcgttagcg aaccaaactg
    11161 cgtatctgcc agaacactgt cagtgttaaa aggtgtaggg accaggacac gaaggcgatg
    11221 atcgtcagcc tggttatcaa ggttgatatc cacatcaata cgcctgctgt tatgactaag
    11281 agtgacaacc aacacccccc ctactctgcc agtggattgc cgggcgctgc gttctgacaa
    11341 attgagcggc actgccatgt catagcggat aacagccctg ctctgccagg cttcatgaat
    11401 aatatcgcat tgcggtttcg cgttcgctgc ggtaattacc cactcttctt ttgcgggtga
    11461 atagtcatat tcatcaccat catcagagct ttcttcaatt tgcaataccc ggtcatagcg
    11521 cacaccgctg tctttatcta ccagttgcag agaaccatcc tcattgagcg caatctgcca
    11581 gaaagcattt tccagaatcc cttcagcgtc actttttgcc gcaatcacgt tgccaggctg
    11641 attcgcttca atataaagcg tgcgatagcc catagaaggg acaatctggt tgatctgaat
    11701 atcaaactcc ataaagggat cataattgcc ataatgaaca atttgccgat cgattaggcc
    11761 aggatcgatc tcacgggcat ggcgaataaa atacggtaca ggctgaccgc gatcgtcttg
    11821 tagattaaac tggctggcgc gcagccgcac agtggtgtta ataacttctt cccgcggcca
    11881 gggcatcagg ttaaacagca cgagtttgtc ggcatcactc tgtggcatgt tatcggcaat
    11941 tttgcgcatg tagaaacgca gcagattatc cgccatgtct tcagccagtt cgaagcgggc
    12001 gacaatttcg cgatgaactt tgtcactgca acaacaaccg atactgtcgt gggcatgatt
    12061 ttttaagatc tctttccaca ttttctccag caagccgtgg tggtattcaa aacccaacgt
    12121 ccaggccagc gttgccagcg gttccagcag attaacaatc ttattttcaa tacgtgcgtg
    12181 ggcaattttg atatccatgc gcgtggaacc gatggtgcga tgtacgcgca tatatttgcc
    12241 atcgataaat tcacctttca gggtggccag actttcacgc tgcgcttcga tcttctcaaa
    12301 tacctcttca aagcggctca tcacaaactt acgttgaggg tagatctcac gtagcttatc
    12361 catcacttcg aagatatttt gctgcaatgg catctggtca tgaccattcg gcagcaaaat
    12421 ctctttggtt acagacgctt tttccagcac gtcaaaataa ctgtcgaggc gtttacgtaa
    12481 tccgttttcg tcggcaggta agtacttacc gatggcgtag cccagcggca gcacctgcgc
    12541 cgtcacttcg ctaccgtcac tgctttgcca caaaaactcg gttttatcag taccgtggcg
    12601 ctccgaacat ccgcgccaga acatagtgcg ggtaatgcca aatccattgt aaatatgcgg
    12661 cagttgcccg gacatgccaa aggaatccgg taaataacct attttcatcg gctcgccaaa
    12721 cgcgaggcag tcacgcattc cgtacatcag attacggacg atggattccg cagaaacaat
    12781 cgtggtatcg gtctgggtat accaggggcc gataatcaac ttaccggctt ctacctgttt
    12841 cttcacgcgg tctttgtttt ccggtttcac cgcgaaataa tcttcgagga tcgccgtttg
    12901 cccgtcgagt acgtaatatt tgtattcgtt gtcctgttcc agtcggcaca ggatctcttc
    12961 catattattg accagcagaa tgcgtgactc ttcggtggtg aaataccact cccgatccca
    13021 atgcatatgc ggggtgatgt gaacgcgaga taccgctttc atcgtcgttt cctttttgtt
    13081 tatggcatta cgccatcagt cagatagttg ccatgcttaa ccgcgtgacg ccgccagatc
    13141 aggagaattg cagtcgagat tgcagccccc accaatgccg caccaaacca gccaattgcc
    13201 gccataacac cgcccgcgcc attatcatga agtaaaaaga gcgagaaaat gccggcaccg
    13261 ggtgtcgaaa ggccgatatt catcgcaccg acaatagcgc ccgttaccat agagcccagc
    13321 acaaacgaac caataacccg cagcggatct tcaatcgcca tcgggatcgc cccttcggta
    13381 atacctgcca gccctaacag ccaggtggat ttcccggtct caatttcaaa ctctttaaac
    13441 aggcgcggtg cgagcatcgt ggaagcggtt acggtaaatg ccgaaaccat tttgacggag
    13501 gcgaaaatgg catacgggcc gtaaacgccg ttcgccattg cgcccaggca gaatgcataa
    13561 gcggctttgt tcactggccc tccaaggtca aaggaacaca taaaaccgag aatggcaccc
    13621 agcaacagcg cgttacttcc tgacagaccg ttcagccagg cggtaagcga gttattgatc
    13681 catgcgacag gttctcccac cacaaacagc atcagactgc ccgctcccaa cgtaccgagc
    13741 accgggtaga gataaaaagt caggaatcca ttgaatttac tgctaagacg caagtgattt
    13801 ttcacccagc gcatcaagta accggctatc aatccgccaa cgaccgcgcc gagaaacccg
    13861 gagccgatca tgttggcggc aagtccagcc gcaaagcctg gcgctaacgc cggtttatct
    13921 gccagagaat aggtggtata ggccgcgagc accggtacca tcaaaattcc gagcagcccg
    13981 ccgcccagct tgcggtacat ccacagccag gagttttctt cattaaacag atcttgtagc
    14041 ccgaagattt gcgacagtaa taccgcgacc gccagcaccg tgccccccgc gacaatcaac
    14101 ggtacggcaa aagagattcc gctcaacagt gcctgtttca gctccgtttt gacacttttc
    14161 accggttgcg tatcttgctg tacggtacgc gtctcatcgc tacgcttgag ggtaagcgct
    14221 tgttggatca acgcttctgc atggcgaatc ggctcggcaa caggcactga aagcgcggga
    14281 atgccattaa aacgctcact ctccttgatg gcgacttcag ccgcaaaaat acaggcggtt
    14341 gcactattga gttgatccgc cgttaaacgc ccttcaatgc cgttagcgcc ttgtttttca
    14401 acgtaaacat ttacgccgag tttgcgtccg gctttttcca gatattccgc agccatatag
    14461 gtgtgagcaa tacccgccgg acaggccgtt acgcagacga tagttggcgc gttggaaaaa
    14521 gaggcagaag gttgcgtgcc tcccttgtca tccagcgccg agagcaactc atcaggcgtc
    14581 gtcgccgact gtatacgcgc ccgaatctca tcatccgcaa ggcgcgtggt cagcgctgtc
    14641 agcagttgca tatgcgtagt acccgcttca ttggggggaa tcgccagcag caccactaaa
    14701 tcaactgctt ccgggccatc aacgccttcc cactgaagcg gctcgctgag tgtggcgacc
    14761 gcaaacgccg cttcttttac cgcagcagtt ttgccatgcg gcacagccaa cccttcacct
    14821 aaggcggtcg ggccaaggct ttcacggcga tacacttctt ccagaaattg ctcagtactg
    14881 gaaattttcc ccagagcagc aagacgttga gtcaacgcgt ggatggcctc ttcacggctg
    14941 gtaaagcgcg cattcagaca caacgcatcg cggtgggtca gagtcgtcag attcatcatg
    15001 atcctcactt gatcgttttt atagtcgcgc cagtgtgccc gataaaacaa taccatcatg
    15061 tgatcaattt cacttgattg aaattatttg tattgtattt gtattattta caggatgaat
    15121 ttatcgggca taatggctaa attcatatta atgagatact aaaaatggga cacaagccct
    15181 tataccggca gattgccgat cgcattcgtg aacaaattgc gcgtggcgag cttaaacctg
    15241 gcgatgcgtt acccactgaa tcagcgctac aaacagagtt tggcgtcagc cgggttacgg
    15301 tgcgtcaggc tttgcgccag ctagtcgagc agcagatcct cgaaagcatt cagggcagcg
    15361 ggacttacgt caaagaagag cgggtcaatt acgatatttt tcagttaacc agttttgatg
    15421 aaaaactgtc ggaccgtcac gtcgatacac acagtgaaat cctgatattt gaagtgattc
    15481 cggctgacga ttttcttcag caacagctac aaatcaccgc gcaggatcgt gtgtggcatg
    15541 tgaagcgcgt gcgctatcgc aagcaaaagc caatggcgct ggaagaaacc tggatgccgc
    15601 ttgcgttgtt cccggatctc acctggcagg tcatggaaaa ttcgaaatat cactttattg
    15661 aagaagtgaa gaagatggtt atcgatcgta gcgaacagga aatcattcct ctgatgccaa
    15721 ccgaagagat gagccgtctg cttaatatca gccagacaaa gccgattctt gaaaaagtat
    15781 cgcgtggata tttagttgat ggtcgggtgt ttgaatatag ccgcaacgct tttaataccg
    15841 atgactataa atttaccctg atagcgcagc gaaaatcatc gcgataagca caaaaaaggc
    15901 catcatacga tggccaacca tgtcgaaacc gacagccctt cgccattcct gtagagggaa
    15961 gcggcgaggg ctatttctta ttacagatat ttatttcaga acagttttca gtgcttcacc
    16021 gatatccgcc aggctgcgaa cggttttcac gcctgcggct tccagagcag cgaatttctc
    16081 atccgcagtc cctttcccac cagcaatgat cgcacccgcg tggcccatac gtttgccttt
    16141 cggcgcagtc acaccagcga tgtaacctac aactggcttg gtaacgtgct ctttgatgta
    16201 cgctgccgct tcttcttcag cgctaccgcc gatctcaccg atcatcacga tcgcttcggt
    16261 ctgcggatct ttttcgaaca tttcgaggat gtcgataaag ttagagcctg ggatcgggtc
    16321 accgccgata ccaacacagg tcgactgacc gaaaccgtaa tccgtggtct gtttaaccgc
    16381 ttcataggtc agtgtaccgg aacgggaaac gatacccact ttacccggtt tgtgaatgtg
    16441 acccggctgg ataccgattt tgcattcacc cggagtgata acgcccgggc agttcgggcc
    16501 gatcatacga acgccggctt cgtccagttt cactttcaca gtcagcatat ccagcgtcgg
    16561 gatgccttca gtgatggtga taatcagttt gatgcctgca tcaatagctt ccagaatgga
    16621 gtctttgcag aacggtgctg gtacgtagat aacagaagcg gtagcgccag tagcagcaac
    16681 agcttcacgc acggtgttga acaccggcag gccgaggtgg gtagtaccgc ctttacctgg
    16741 ggttacgccg ccaaccattt tagtgccgta tgcaatggcc tgttctgagt ggaaagtccc
    16801 ctggctacca gtaaagccct ggcagataac cttggtgttt ttatcaatta aaatggacat
    16861 tatttcccct ccactgcggc aacaacctgc tgagctgcat ccgtcagacc ttttgctgca
    16921 ataatattca ggccgctgtc agccagtttc ttcgcgccga gttcggcgtt gttaccttcc
    16981 agacgtacca cgaccggtac gttaacaccc acttctgcta ccgcgccgat gataccgtca
    17041 gcgatcaggt cgcaacgaac gataccgccg aagatgttaa ccagaacggc tttcactttg
    17101 tcgtcagaga ggatgatttt gaacgcttcg gttacacgtt ctttggttgc gccgccgcca
    17161 acgtcaagga agttagccgg ttcgccgccg tgcagtttaa cgatgtccat cgtacccatc
    17221 gccagacctg cgccgttaac catacaaccg atgttaccgt ccagcgcaac gtagttcagt
    17281 tcccactgtg cagcctgtgc ttcacgcgga tcttcctgcg actggtcacg catttcgcgc
    17341 agatcaggct ggcggaacag tgcgttgccg tcagcgccca gtttgccgtc gaggcaaatc
    17401 agatcgccct gtttggtgat gaccagcggg ttgatttcga tcaacgccag gtcgcgctcc
    17461 aggaaaatgg tcgccaggcc catgaagatt ttggtgaact gctgaaccag tttaccttcc
    17521 agacccagtt tgaacgccag ctcgcgtccc tgatacggca tcgggccagt cagcggatca
    17581 agcgcaactt tgtggatcag gtgcggagtt tcttccgcca ctttttcgat ttccacgccg
    17641 ccttcggtgg aggccataaa gaccacacga cgggaactac ggtctacaac ggcaccgaga
    17701 tacagctctt tagcgatatc ggtcgctgct tcaaccagaa tctggttaac cggttggcca
    17761 ttggcatctg tttgatacgt taccagacgc ttgccgagcc agttttctgc aaaagcacgg
    17821 atgtcttctt tgctgtttac aactttcaca ccgcccgctt taccgcggcc accagcgtga
    17881 acctgacatt tcactaccca cgggccggca ccgatttttg aagcggcttc ttctgcttcg
    17941 cgcggagtag tacaggcata acccaccggt gctggtaagc catagcgggc aaaaagttgt
    18001 tttgcctgat attcatgtaa gttcatgtgt tctgtccatc cttcagtaat cgttatcttt
    18061 taaaccgtag accgacaccc tatccacgtc ggtctacagt gcaggtgaag cttaaactac
    18121 tacacgtcca gcagcagacg cgtcggatct tccagcaact ctttgatcgt taccaggaag
    18181 cccacggatt cgcgaccatc gatcagacgg tgatcgtagg acagcgccag gtacatcatc
    18241 ggcaggatct caacctgacc attcaccgcc atcggacgat ctttgatagc gtgcataccc
    18301 agaattgcgc tctgcggcgg gttgatgatc ggcgtagaca tcagggaacc gaacacacca
    18361 ccgttggtga tggtgaagtt accaccggtc agatcttcaa ccgtcagctt gccgtcacgg
    18421 cctttaactg ccagctcttt gattttcttc tcgatgtctg ccatgccgag ggtatcgaca
    18481 tcacgcagta ccggcgtcac caggccgcgc ggcgtggaaa ccgccatgct gacgtcgaaa
    18541 tagttgtggt aaaccacgtc atcaccgtcg atagaagcat tcacttccgg gtaacgtttc
    18601 agggcttcaa ccaccgcttt cacgtagaag gacataaagc ccagacggat gccgtggcgt
    18661 ttttcaaacg cttcaccgta ctgcttacgc agatccataa tcggcttcat gttgacttcg
    18721 ttgaacgtgg tcagcatggc ggtggagttt ttcgcttcca gcagacgctc tgccacacgc
    18781 ttacgcaggc gagtcatcgg gacacgtttt tcactacgtg cagccagagc cggttgcgcc
    18841 gccggagcag ccgctgccgg agcagactct ttcgccgggg ctttcgccag atgtttttcc
    18901 acatcttcac gagtcagacg accacccaca ccggtgcctt taatggcgct ggcgtcgaga
    18961 ttgtgttcag ccagcaggcg acggatcgcc gggcttaacg catcgttgtt ttgctcttcc
    19021 agagacgcct gctggcgttg cgccggagtg gacgctttct cttcagattt ggcgctggtt
    19081 tctttaccgg cgctgttgcc ttcacgcagg cgaccaagga tctgacgaga cgttaccgtt
    19141 gtaccttcat cttccagaac cgcatccaga atgccgtctg ctgatgccgg tacttccagt
    19201 accactttgt cagtttcgat ttctaccagc acttcatcac gtacgactgc gtcgccgggt
    19261 tttttatgcc aggttgcgac ggtggcatcg gctacggatt caggcaggtc agggaccaga
    19321 atatctacgc tactcattgt gtatccttta tttattcgac gttcagcgcg tcattaacca
    19381 gatcttgttg ctgtttctgg tgaacggaca tataccctac cgccggagag gcggaggccg
    19441 tgcggcctgc ataacgcaga gaagccccaa acggaatcac ttcacggaaa tgatgctggc
    19501 tgcagtacca tgcgccctgg ttgagcggct cttcctggca ccagacaaaa tccttgacgt
    19561 gagcaaactg ctgcaacact tcctgcatcg ctttatgcgg gaacgggtag agttgctcga
    19621 tacgcacaat ggcgacatcg tgttgattgt tcttacgacg ctgttccagc aggtcgtaat
    19681 aaaccttacc agaacacatc actacgcgct tcacgccctt cggatcaagc tcgtcgattt
    19741 caccgatggc tggcaggaag gtgccgttcg ccagttcttc gaggctggaa accgccagcg
    19801 gatgacgcag cagggatttc ggcgacatca ctaccagcgg acgacgcatc ccgcgcagcg
    19861 cctgacgacg cagcatgtgg taaacctgtg ccggggtaga cggaacgcaa acctgcatgt
    19921 tttgctcagc acaaagttgc agataacgtt ccagacgcgc ggaggagtgc tccggcccct
    19981 gcccttcgta accgtgcggc agcaacatca ccagaccaca catccggccc catttctgtt
    20041 cgccagaaga gatgaactgg tcgataacca cctgcgcacc gttggcgaag tcaccgaact
    20101 gcgcttccca gatggtcaga gtgcgtggtt ctgcggtggc ataaccgtat tcaaacgcca
    20161 gcactgcttc ttcagacagt acggagtccc agacgcggaa cgcgccctgc ccgttatgga
    20221 tgtgttgcaa cggcgtgtaa gtggaaccgt tagactggtt gtggatcacc gcgtggcggt
    20281 ggaagaaggt accgcgaccg gagtcttcac ccgacaggcg aaccggaatg ccttcgtcaa
    20341 ccagcgtggc gtaagcgagg ttttccgcac cgccccagtc gaacagtttc tcaccggcag
    20401 ccatcgcctg gcgatcgcca taaatcttgg caacgcgaga ctgcatttca actgcttccg
    20461 gcaccgtgct gatgcgtttc gccagctcct gcaggcgctt catctcaact ttgttcgggt
    20521 actcttcgtc ccattcgtgg ttgaggtacg gcgaccaggt gaaagagtgc atgttcatcg
    20581 gacgccactc tgccactacg caatcgccag catccagcgc atcgcggtac aggttaacca
    20641 tctcggtggc atcttccagc gtcgccactt tttcctgctc cagcttgtca gcgtagattt
    20701 tgcgcggtgt cggatgtttt ttgattttct gatacatcag cggctgggtt gcgctcggct
    20761 cgtcggcttc gttgtggccg tgacggcggt agcacaccag gtcgatgaag acatcacgtt
    20821 taaaggtgtt acggaaatcg agcgccagac gggtcacaaa ggcaacggct tccggatcat
    20881 ccgcgttaac gtggaaaatc ggtgcctgaa ccatcttacc gatatcagta cagtacggtg
    20941 tagaacgagc atccagtgga ttagaggtgg tgaaaccaac ctggttgttg ataacgatac
    21001 gtaccgtacc gccaacttca taaccacgcg ctttcgacat gttcagagtt tcctgaacca
    21061 cgccctgccc ggtcactgcg gcgtcaccgt ggatggtgat tggcagcact ttgttgctgc
    21121 tcggctcatc aagtctgtcc agacgggcac gaacagaacc gataactacc gggcttacaa
    21181 tctcaaggtg agacgggtta aacgccagcg ccaggtgcac caggccgcca tcggtctgga
    21241 agtcagacga gaagcccatg tggtatttca cgtcacccgt gccgaggtgt tctttatgtt
    21301 taccggcaaa ctcgtcgaac aagtcttgcg gttttttacc cagcacgttc accagcacgt
    21361 tcagacgacc acggtgcgcc atcccaagaa ccacttcgcg ggtgccgctg ttgccagcgt
    21421 ggcggatcat ctctttaagc atcgggatta acgcgtcacc gccttccagc gagaagcgtt
    21481 ttgcgccagg gaattttgcg ccgaggtaac gttcaaggcc ttcagcggcg gtcagttcgc
    21541 ttaagaagcg ttttttctct tcgctattga aagtcgcgcg accagactcg atacgctgtt
    21601 ggatccagcg tttttcttcg gtgctggtaa tgtgcatata ctcggcacca atcgggccac
    21661 agtaggtttg cttgagggct tccagcagct cgccgagttt catgttttct ttgccgctgg
    21721 caaatgaacc gacgttgaag gtctcctgga agtctgcttc ggtcagatcg tggaaagacg
    21781 gatccagatc ggccacttta tcttgctgcc acagtcccag cggatcgaga ttcgcatgct
    21841 ggtgaccacg gaagcggtat gcgttaatga gctgcaggac tttaacctgc ttcacattgg
    21901 tgtcagggtc ggagatcgtt gaagagtaac gtgaagcgtc tttcgccagg cggcggaaat
    21961 attcacgcgt ttgagagtgg aattgatccg gtttgactcc cgtaccaggt aactgctgga
    22021 acgtcgaacg ccagttagcg tcaaccgagt caggatcggt taagaagtct tcatagagct
    22081 gttctatcca gctctggttt gcgccagaga ggtaagaaga gtccaaccag gctttcaaag
    22141 cgctgttctg catcgtgatc ccttaagcat cttttttatg cttacttcgc cgtggatact
    22201 accacgcaca gtgatgtgcg tgccggggtt cacttgcgag ctcttgtcta tgtagtggct
    22261 cgcgaaggaa cctttaaaaa ctgtcttagt gtcaggggca gtttttaaag atttcctgca
    22321 tttctttgtt acgcctgatg cgctgcgctt atcaggccta cgggtaatga cttgtaggcc
    22381 ggataaggcg ttcacgccgc atccggcact ggttgcctga tgcgacgctt gcgcgtctta
    22441 tcaggcctac ggtttacgca ttacgttgca acaacatcga cttaatatgg ccgatggcgc
    22501 gcgtcgggtt cagccccttc ggacatacac tgacgcagtt catgatgctg tgacagcgga
    22561 atacgctgaa tgcatcgctc aaaccgtcga ggcggctgtc agtctcggta tcacggctat
    22621 cgatcaggaa acgatatgcc gctaacaagc ctgccgggcc gataaactta tcgggattcc
    22681 accagaaaga cggacaagag gttgaacaac atgcgcagag aatacattca tacagcccgt
    22741 cgagtttttc gcgctgctct ggcatctgta aatgctcgcg agctggcgga ttttgtccat
    22801 tattcaacag gtaaggctta attttctcat attgcgcata gaattgtccc atgtctacca
    22861 ccaaatcgcg gatcaccggt aaacctggca gcgggcgaat cacaatcttc ttgcccggct
    22921 ggttgagtgc cgaaatcggg gtaatacagg ccagaccatt cttgccgttc atgttcagac
    22981 cgtcggaacc gcacacacct tcacggcagg agcggcggaa cgacaggctg ggatctttct
    23041 ctttcagctg gataagcgca tccagcagca tcatgtcgcg accttcttcc gcttccaggg
    23101 ggtaatcctg catacgcgga gcatcatcaa catccgggtt atagcgataa attgaaaact
    23161 cgagtctcat tttcctgtct ccgcattagt aagtacgaat cttcggcggg aatgccgggc
    23221 gcagtttcgg ttccatgttg acgcttcggc gcgtcatgga ttccgactct ggcagataca
    23281 gggagtggca cagccagttt tcatcatcac gatccgggaa gtcgaagcgg ctatgtgcgc
    23341 cacggctttc ggtacggaag ttggcagaaa cagccgttgc atacgccgtt tccatcaggt
    23401 tatccagttc caagcactca acgcgctggg tgttgaactc gctggaagtg tcatccagac
    23461 gggcattttt cagacgctcg cggatcactt tcaactgctc aagccctttc gccatcgcat
    23521 caccttcacg gaagaccgag aagttatgct gcatacattc ttgcagcgct ttacgaatcg
    23581 ccaccggatc ttcaccgtta cgattattgt tccagcggtg cagacgatcc agagatgctt
    23641 caacatcaga ctcgctggca tcgcgcaatg cgccctgttc ggcgatagac tcttgcagat
    23701 gcagacctgc cgcacgacca aagaccacca ggtccagcag cgagttgccg cccagacggt
    23761 tagcgccgtg taccgataca caagcgattt caccaacggc aaacagcccc ggaacaacca
    23821 catcttcgcc tttctcgttc accgtcagcg cctgaccggt cactttggtc ggaataccgc
    23881 ccatcatgta gtgacaggtt gggataaccg gaatcggctc tttcaccgga tcgacgtgag
    23941 caaaggtacg ggaaagctcc aggatacccg gcagacggga ttcgagaact tctttaccca
    24001 ggtgatcgag tttcagtttc gcgtgtggcc cccacggacc atcacagccg cgaccttcac
    24061 ggatttcaat catgatggaa cgcgccacca catcacggcc cgccaggtct ttggcgttcg
    24121 gcgcataacg ctccataaag cgttcgccat gtttgttcag cagataaccg ccttcaccac
    24181 ggcaaccttc ggtgaccagt acgcccgcac cggcaatgcc ggtcgggtgg aattgccaca
    24241 tttccatatc ctgcaccggt acgcctgcgc ggatagccat gccgacaccg tcgccagtgt
    24301 taatgtgggc gttagtggtg gactgataaa tacgccctgc cccgccagtc gccagcacgg
    24361 tagcgcgggc tttgaaataa accacttcac cggtttcgat gcacagtgcg gtacaaccca
    24421 ccaccgcgcc atcctggttt ttcaccagat ccagcgcata ccactcggag aaaatggtgg
    24481 tgtggttttt caggttctgc tgataaagcg tgtgcaacag tgcgtgaccg gtacggtcag
    24541 ccgccgccgc agtgcgtgcc gcctgctcgc cgccgaagtt tttcgactga ccgccaaacg
    24601 gacgttgata gatacgacca tcatcaagac gcgagaacgg caggcccatg tgttcgagtt
    24661 ccagaatcgc ttccggcccg gttttacaca tatattcaat cgcgtcctgg tcaccgatat
    24721 agtccgaccc ttttacggtg tcgtacatat gccattccca gttatcttca tgggtattac
    24781 ccagcgcaac ggtaatgccg ccttgcgcag aaacggtatg ggaacgggtc gggaagactt
    24841 tagagagcag cgcacaggtc tggccgctct gggaaatttg cagcgccgcg cgcatacctg
    24901 cgccaccggc accaatcaca actgcatcaa attctctgac tggcaatttc atcacacacc
    24961 ccacaccaca acgaatccat aaatcacgta aaccaccagt gcaacgacaa tcaccagttg
    25021 cagcatcagg cgcaatgcca gcggtttaac gtagtcggtc aacacctgcc acatgccgat
    25081 ccaggcatga atcaagatag aaaacagcgc cagcagggtg aacactttgg tgaacgcaga
    25141 ggcgaagaaa ccgatccaga cttcatatgt cagttcgcca ctggtggcga aaaaaccgac
    25201 catataaatg atgtagagcg tcaggacgat agcggtagcg cgaacgagga tgaaatcatg
    25261 tacgccattg cgtcctaatg cggaggcgtt gcttaccata cgaggactcc tgcgagaagt
    25321 gaaagcacga cagtaataac aaaggagatt ttggcggagc gtttacccgc ttcgaatgtt
    25381 tcttccagat agccaaaatc catcatcatg tggcgaatac ctacgacgac gtgatacgcc
    25441 agagcggtaa ggatgcccca catgataaat ttgacgaaga agctgcccat aatcgcggaa
    25501 gcttgctcga aaccttcagg ggaagagagg ctggtaccca gaagccacag caggatgccc
    25561 actgcaacaa aggtgatcac accggaaacg cgatggagaa tggacgctat cgccgtgatg
    25621 gggaaccgga tggtctgtag gtccagatta acaggtcttt gttttttcac atttcttatc
    25681 atgaataacg cccacatgct gttcttatta ttccctgggg actacgggca cagaggttaa
    25741 ctttctgtta cctggagacg tcgggatttc cttcctccgg tctgcttgcg ggtcagacag
    25801 cgtcctttct ataactgcgc gtcatgcaaa acactgcttc cagatgcgaa aacgacacgt
    25861 tacaacgctg ggtggctcgg gattgcaggg tgttccggag acctggcggc agtataggct
    25921 gttcacaaaa tcattacaat taacctacat atagtttgtc gggttttatc ctgaacagtg
    25981 atccaggtca cgataacaac atttatttaa tttttaatca tctaatttga caatcattca
    26041 acaaagttgt tacaaacatt gccaggaaaa gcatataatg cgtaaaagtt atgaagtcgg
    26101 tatttcacct aagattaact tatgtaacag tgtggaagta ttgaccaatt cattcgggac
    26161 agttattagt ggtagacaag tttaataatt cggattgcta agtacttgat tcgccattta
    26221 ttcgtcatca atggatcctt tacctgcaag cgcccagagc tctgtaccca ggttttcccc
    26281 tctttcacag agcggcgagc caaataacaa acgggtaaag ccaggttgat gtgcgaaggc
    26341 aaatttaagt tccggcagtc ttacgcaata aggcgctaag gagaccttaa atggctgata
    26401 caaaagcaaa actcaccctc aacggggaca cagctgttga actggatgtg ctgaaaggca
    26461 cgctgggtca agatgttatt gatatccgta ctctcggttc aaaaggtgtg ttcacctttg
    26521 acccaggctt cacttcaacc gcatcctgcg aatctaaaat tacttttatt gatggtgatg
    26581 aaggtatttt gctgcaccgc ggtttcccga tcgatcagct ggcgaccgat tctaactacc
    26641 tggaagtttg ttacatcctg ctgaatggtg aaaaaccgac tcaggaacag tatgacgaat
    26701 ttaaaactac ggtgacccgt cataccatga tccacgagca gattacccgt ctgttccatg
    26761 ctttccgtcg cgactcgcat ccaatggcag tcatgtgtgg tattaccggc gcgctggcgg
    26821 cgttctatca cgactcgctg gatgttaaca atcctcgtca ccgtgaaatt gccgcgttcc
    26881 gcctgctgtc gaaaatgccg accatggccg cgatgtgtta caagtattcc attggtcagc
    26941 catttgttta tccgcgcaac gatctctcct atgccggtaa cttcctgaat atgatgttct
    27001 ccacgccgtg cgaaccgtat gaagttaatc cgattctgga acgtgctatg gaccgtattc
    27061 tgatcctgca cgctgaccat gaacagaacg cctctacctc caccgtgcgt accgctggct
    27121 cttcgggtgc gaacccgttt gcctgtatcg cagcaggtat tgcttcactg tggggacctg
    27181 cgcacggtgg tgctaacgaa gcggcgctga aaatgctgga agaaattagc tccgttaaac
    27241 acattccgga atttgttcgt cgtgcgaaag ataaaaatga ttctttccgc ctgatgggct
    27301 tcggtcaccg cgtgtacaaa aattacgacc cgcgcgccac cgtaatgcgt gaaacctgcc
    27361 atgaagttct gaaagagctg ggcaccaaag atgacctgct ggaagtggct atggagctgg
    27421 aaaacatcgc gctgaacgac ccgtacttta tcgagaagaa actgtacccg aacgtcgatt
    27481 tctactctgg tatcatcctg aaagcgatgg gtattccgtc ttccatgttc accgtcattt
    27541 tcgcaatggc acgtaccgtt ggctggatcg cccactggag cgaaatgcac agtgacggta
    27601 tgaagattgc ccgtccgcgt cagctgtata caggatatga aaaacgcgac tttaaaagcg
    27661 atatcaagcg ttaatggttg attgctaagt tgtaaatatt ttaacccgcc gttcaaatgg
    27721 cgggttaatt tttatatgcc caaacacaaa aaattgtaaa aataaaatcc actatcggac
    27781 attcatatat tctaaaaaaa atatatcggt ataaattata aacaaatcga tgatttttaa
    27841 tcatctatct tcctctcagt tacatgaata aaccaccaaa tatagcgcac ccctataatt
    27901 tccaatactt tattcatcaa acatttaatt cataccatca attaattact tttacattta
    27961 ataaaattaa taatattttt aagaaaaact aaaaacattc gacaatcaaa taagatattc
    28021 ttgcaccgat atactttttt cttttctgtt aaaggactca gttatgttta aaggacaaaa
    28081 aacattggcc gcactggccg tatctctgct gttcacagca cctgtttatg cggctgatga
    28141 aggatccggt gaaattcact ttaaaggtga agttattgaa gcaccgtgtg aaatacatca
    28201 ggatgatatt gataaagagg ttgaactcgg tcaggtgacc accagccaca ttaatcagtc
    28261 acatcacagc gatgccgttg ctgtcgacct gcgcttagtc aactgtgatc tggaaaactc
    28321 cagcaacggt tccggtggca agatttctaa agttgcagtc acctttgata gctcagcgaa
    28381 aaccaccggc gcagatccga ttctcaacaa caccagcacg ggtgaagcca ccggcgtcgg
    28441 cgtacgttta atgaataaag atcaaagcaa tatcgtttta ggcaccgcta ctccagatat
    28501 cgacctggct ccgacctcca gcgaacaaac gctgaatttc tttgcctgga tggaacaaat
    28561 tgatcaggct acacctgtaa cgccaggcgc agttacggcg aatgcaacct acgtgctgga
    28621 ttataaataa gtattacttt ggcggcgcta tcgccgcctt tcataaaata tttatcaggt
    28681 atggacaccg tgaatattta tcgactctct tttgtatcct gcctggtcgt ggcgatgcct
    28741 tgcgcattgg cggtcgaatt caaccttaat gttctcgata aatcgatgcg cgaccgcatt
    28801 gatatttcat tattaaagga aaaaggagtc attgctcccg gtgagtattt tgttagcgtt
    28861 gcggtaaata ataaccaaat cagtaacggg caaaagatta actggcacaa aaatgacgat
    28921 aaaaccattc cgtgcatcaa tgatttactg gtcgataaat ttggcttaaa acctgaagtc
    28981 cgtcagtcgt taccattgat aaatcagtgc gtcgatttta gctcccgacc tgaaatgctc
    29041 ttcaatttcg atcaagccaa tcagcaactg aatattagta ttccgcaagc ctggctggcg
    29101 tggcactcag aaaactggac cccaccctcc acatggaaag aaggtgtcgc cggtatcctg
    29161 atggattaca acttgtttgc cagcagctac cgcccacagg acggcagcag cagcactaac
    29221 ctgaacgcct acggtaccac cggaattaac gccggggcat ggcgcttacg tagtgattat
    29281 cagttgaatc agactgatag cgatgataac catgaacagt caggcggaat atcgcgcacc
    29341 tatctttttc gtccattacc gcaattaggc tctaaattaa ccctcggcga aacggatttt
    29401 agttccaata ttttcgacgg tttttcttat accggcgcgg cactggcaag tgacgagcga
    29461 atgttgccat gggaactacg cggctacgcc ccacaaatta gcggtattgc acagaccaat
    29521 gccacggtga cgatcagtca atcaggccgc gtcatttacc agaaaaaagt cccaccaggc
    29581 ccatttatca ttgacgacct taatcagtct gttcagggca cactggatgt caaagtgacg
    29641 gaagaagatg gtcgggtgaa caatttccag gtttcggcag catcgacgcc cttcctgact
    29701 cgtcagggac aggttcgcta taaactggcc gcgggtcagc cacggccctc catgtcacat
    29761 caaactgaaa atgaaacctt ttttagcaat gaagtttcct gggggatgct gtcaaacacc
    29821 tcgctgtacg gcggcctgct gctttctggt gatgactacc attctgccgc aatgggtatt
    29881 gggcaaaata tgctgtggct tggtgcgctg tcgtttgatg tcacgtgggc cagtagccat
    29941 tttgatactc agcaggacga gcggggctta agctaccgtt ttaattacag caaacaagtg
    30001 gatgccacta acagcacgat ttccctcgcc gcttatcgtt tctccgatcg tcattttcac
    30061 agctacgcca actatctgga tcacaaatac aacgacagcg atgcgcagga cgaaaaacag
    30121 acgatcagct tattgtgggc caaccgatta ccccactaaa cctcaatctt tacgccaacc
    30181 tgctacatca aacctggtgg aatgcagacg cctccacgac cgccaacatc acggcaggtt
    30241 ttaatgttga tattggtgac tggagagata tctctatttc gacgtcattc aatacaaccc
    30301 attacgaaga taaagatcac gacaaccaga tttacctgtc gatttcgctc cccttcggta
    30361 acggtggtcg ggttggctat gacatgcaaa acagtagcca cagcaccaca caccgcatgt
    30421 cgtggaacga tacgctggat gaacgtaata gctggggcat gtctgccgga ctgcaatccg
    30481 accgtcctga caatggagcc caggtgagcg gtaactatca gcacctgagt tcagcgggtg
    30541 agtgggatat ttctggtacc tatgccgcca atgattacag ttccgtcagc agcagctgga
    30601 gcggttcttt caccgcaacc caatatggtg cagcgtttca tcgccgcagc tccaccaatg
    30661 aaccacgcct gatggtcagc accgatggcg tggcagatat tccggttcag ggcaatctcg
    30721 actacaccaa ccattttggc attgcggtgg tgccgttgat ttccagttac cagccttcca
    30781 ccgtggcggt gaacatgaat gacttacccg acggcgtaac agttgcagaa aacgttatca
    30841 aagaaacgtg gattgaaggc gcgataggtt acaaatcact ggcttcccgt tccggtaaag
    30901 acgttaacgt catcattcgc aacgccagcg gtcagtttcc tcccctcgga gcggatatcc
    30961 gccaggatga cagcggcatt agcgtgggga tggttggcga ggaaggacat gcctggttaa
    31021 gcggagtcgc tgaaaatcaa aagtttaccg tggtctgggg tgatagccag cattgctcgc
    31081 tccatcttcc tgaacatatg gaagacaccg caaatcgcct gattttacct tgtcattaat
    31141 taaggaaatg aaaatgacat ttatgaaagg actacccctt ttgttgttgg ttgccagtct
    31201 gtgcagccac gctgcactac aacccgatcg cactcgtatt gtgtttaacg ctaatgataa
    31261 agctaccagt ctgcgggtgg ataatcgtag cgataaactc ccctacctcg cttattcctg
    31321 gctcgaaaat gaaaaagggg agaaaagcga tgatcttctg gtcgccctgc cgccgataca
    31381 acgtctggaa ccgaaagcaa caacgcaggt acggatagta aagcaagcct cgaccacaaa
    31441 attaccgggc gatcgcgaaa cgctattttt ctacaatatg cgtgaaattc ctcccgcccc
    31501 ggaaaaaaac agcgaccatg ccgttctgca agtcgccatt cagagtcgta tcaaggtgtt
    31561 ctggcgaccg gctgcattac gcaagaaagc aggagaaaag gtcgaactgc agttacaggt
    31621 cagccagcag ggcaaccaac tgacactgaa aaatcctacc gcgtattatc tgaccattgc
    31681 ctacctggga cgaaatgaaa aaggcgttct ccccggcttc aaaaccgtga tggttgcgcc
    31741 ttttagtacg gtcaacacaa acaccggaaa ttatagcggc agccagtttt atctcggtta
    31801 tatggatgat tacggcgcgt tacgcatgac gacgctcaac tgcagcggac aatgccattt
    31861 acaagccgtg gaggcgaaga aatgagtgct ggcaagggag tgttgctcgt catctgtctg
    31921 ttatttctgc cgttgaagtc tgcgcttgcc ctgaactgct actttggttc atccggtggc
    31981 tcggtagaaa aatcagaagc gattcaacct tttgctgtac caggcaatgc caaactcggc
    32041 gataagattt gggaatctga cgatattaaa attcccgtct attgtgacaa caataccaac
    32101 ggcaattttg aaagtgagca tgtttatgcc tgggtaaacc cctacccggg ggtgcaggat
    32161 cgctattatc aactcggcgt aacttacaat ggcgttgatt atgacgccaa ccagggaaaa
    32221 agccgcatcg acactaacca gtgtattgac agtaagaaca ttgacattta tacccctgag
    32281 cagattatcg cgatgggatg gcagaacaaa atttgctcgg gtgatcccgc taatattcac
    32341 atgtcgcgta cctttctcgc tcgcatgcgg ttatacgtca aaatacgaga aatgccgccg
    32401 catgattatc aaagtacgct tagcgactat atcgtcgtgc aatttgacgg tgccggtagc
    32461 gttaacgaag accctactgc ccaaaacctg aaatatcata ttactggtct ggaaaacatt
    32521 cgcgtgctgg attgcagcgt caatttttcc atttcaccgg aaacacaggt gattgatttt
    32581 ggtaaattta atttgctgga tattcgtcgc cacacaatgt cgaaaacatt cagtattaag
    32641 acgacaaaat cacaaaatga ccaatgcacc gacggattta aagtcagttc ctccttttat
    32701 accgaagaaa cgttggttga agaagataaa gcattgttga ttggtaatgg tctgaagcta
    32761 cgtttgctgg atgaaaacgc ctcgccttat accttcaata aatatgctga atacgccgat
    32821 ttcaccagcg acatgttggt ctacgaaaaa acctatacag ctgaactttc gtccattgca
    32881 ggtaccccca tcgaggctgg gcccttcgat acagtggtgc tttttaaaat taactataac
    32941 tgatttgcca tgatgccctt tgcagccgga aagggcatct ctttcagaaa attgctgatt
    33001 ttcccctttt ccttgattgc tgttattttt tcctggcccg gctttcttta tgctgtatcg
    33061 atacaccaaa gagaaaataa tgagggagcg aaggatgcca gttttgcagt ggggaatgtt
    33121 atgtgtgcta tcacttctcc tttctattgg tttcctcgcg gtacatctcc cggcggcgct
    33181 attacttggg ccgatgatcg ccgggatcat cttcagtatg cgcggaatta ctctgcaact
    33241 tccccgctcc gcttttctcg ccgcgcaggc cattcttggc tgcatgattg cgcaaaacct
    33301 cactggttcg attctcacca cgctggccgc caactggccg atcgtgcttg ctattttact
    33361 ggtgacgctg ctttccagcg ccatcgtggg ttggttattg gtgcgctata gctcactgcc
    33421 tggaaatacc ggtgcctggg gttcttctcc aggcggcgca gcggcaatgg tcgctatggc
    33481 gcaagattac ggcgcagata ttcgcctggt tgcatttatg caatatctgc gagtgctgtt
    33541 cgtcgcgggg gccgctgttc tggtcacccg catgatgctg ggtgataacg ctgaagcggt
    33601 taaccagcaa atcgtctggt tcccgccagt gagcattaat ctcctgctta cgattttgct
    33661 cgcagtcgtt gccggtacgg cgggatgcat gctgcgtctt ccttccggca cgatgctcat
    33721 cccgatgctg gcgggcgcag tgctccagtc tggtcagctc atcaccatcg aactaccgga
    33781 atggctgctg gcgatggcgt atatggcaat tggctggcgg attggtcttg gtttcgataa
    33841 acaaatctta ctgcgggcac tacgcccgct accgcaaatc ctgctgtcga tttttgctct
    33901 gctggctatt tgtgcgggta tggcgtgggg gctgacccgg tttatgcaca ttgattttat
    33961 gactgcctac ctcgccacca gtcccggcgg gcttgatacc gtggcggtca tcgccgcagg
    34021 gagcaatgcc gatatggcgc ttattatggc gatgcaaacc ctgcgcctgt tcagtatttt
    34081 gctgacgggg ccagccattg cgcggtttat ttcaacctat gcgccgaagc ggtcggccta
    34141 gtgctggcag ccagggcacc agtaaaacgg gcgagatgac agcgtggttt tctcaatgat
    34201 gctgccacag cgttcgcacg gttcgccatc acgatgaaaa accttaaagc gaaacagcgc
    34261 cccatgatgc ttattctcat ccaccagccc cctcgtagcg taggaaaatc gaggaatatc
    34321 cagtaacgcg tgtgcgagtg catccagttg cgccgcattg agatctttcg ctttatgatt
    34381 tccagtcaac ccaacctgcc agaggatctc cacccgcaaa taattgccaa gcccagccag
    34441 aaacgcctga tcgagcagta atcctgcaaa ctgacggtta cgaaagcgcg gtgacaataa
    34501 ccgttccttt accacctccg gcgtcagatt cggatccagc acatcgggac caacgcgttg
    34561 taaaaacgga tgcgtggtca gttgttccgg cctcaacatc tcaatatccg aggcgctata
    34621 aagcagaatg gttttgtcag ccgtttgcag ttttacccgc aatactcgcg tggtctgcgg
    34681 ctcttcgccg gtatcaacca cgcgccagac gccgtaaagc tgattatggc tgtagagcgt
    34741 taagtcgttg gaaaaatgag ttaacaacgc cttaccacgc gtttccacat gggtaacgtg
    34801 ttgaccgata agttgtgatt gataagtttt taactgcggg aaggcaaacc agacatcagt
    34861 tagtggtttg cctttgatcg ccgcctccag gttatccgct gcacggcgga tctccgggcc
    34921 ttcaggcatg gtgatatcct tttttcattc tttattaatc ggggttatgc tgcgacaaca
    34981 atctcctttt cggcaaatgt agagcgtagt cggcgggcga acgccagtgc gtgctcgccg
    35041 tcgccatgca ggcagactgt ttgcgccgtg accgttgccc attcgccggt gatacttttg
    35101 actctgccgt gttgcaccat ctctagcgtt tgcgccaacg cctgttcttc gttttcaatc
    35161 aacgcgcctc gctgacttcg cggcaccagc gagccgtcag cctgataacc gcgatcggca
    35221 aacacttcct cgcgcgttgt caggccatat tgcttgcctg cacgaatcag ctcgcttcct
    35281 gccagcccaa cgagaatcaa tgccggatcg caagcgtata ccgctctggc gatggcgtct
    35341 gccagttgtg cttctttcgc cgcctggttg tacaacatgc cgtgcggttt gacatgacgc
    35401 attacgccgc cttgcgcacg ggcaatcgtt gccagcgcgc caatttgata cagcgtctgg
    35461 gcgtaaacgg tttctggcgg cagctgcatg gcgctgcgac caaaattttc cctgtcggga
    35521 aaactcgggt gagcgccaat cgcgacacca ttttttattg cttcacgcac gcaagcctgc
    35581 atgatttgcg catcgcctgc atgaaatcca caggcaatat tggcagagga aaccagcgtt
    35641 aatagctctg cgtcgctggc gcagccttcg cccagatcgg cgttcaggtc aattttcatt
    35701 gtgcagccgc cacactaatt gttcgaaata acgttgctga tcttgccgcg ctttcagtgc
    35761 ctcttccagt gaacactgga caaaatgaat cggctgaccg agcggaattt gcgccagatg
    35821 gtacatatca gcctcaatga tacaggcaat acgcgggtaa ccatcggtgg tctgtgcgtc
    35881 gttcatcaac acaatcggct gcccgttatg tggcacctgc accacgcccg gtaacaaacc
    35941 gtgagataac agttcgcgat cggtggtgcg ttttaaaatt tgcccctgta agcgatagcc
    36001 catgcggtta ctttgcgagc taagctgcca gggcgaacgc cagaatgcat cctgcgaggc
    36061 gcgatcgaac tcatgatatt ccggccccgg caaggcgcga atgcggttgc cccacagcag
    36121 ctgtttaacg ccctgcgctt ccataaaatc acgcttcgat ttgccaatcg ggagtcggtc
    36181 accatccttc agtaaacggc cttccagccc gccaatcccc actttgagat cggtgctgca
    36241 tgaccccatt accggcggaa catcaatacc acccgcgacc gccagataac tgcgcatccc
    36301 gtgctgcggg cgtttaagcg ttaaacgctg gcctgctttc atcggcaatc gccagccggt
    36361 ccagacggca ttatcatcca gccgcgcttc gcaaccggca cccgtcagag caaaccaccc
    36421 atcagtttcg aactcaaccg ttaactgacc gagcgtgatc tccagcgcgg gggcattggc
    36481 gtcattaccc accagcaggt tagcaatgcg taacgcaggc atatccagtg cgccgcagtg
    36541 gctgattccc gactggcgaa aaccgtgacg accgccatcc tgcacagtgg tatacatgcc
    36601 cgcacgaata atcttcagca tactccctcc ttctgcggta caaagcgcac gctgtctccc
    36661 ggacgtaata agatgggttc gtcacgcgcc ggatcaaaca ggctgagtga ggtatgacca
    36721 atcaactgcc agccacccgg cgttgccagc ggataaacac cagtctgcgg cccgccgatc
    36781 ccgacagaac ctgccggaac gagcaagcgc ggttcagcgc gccgtggcgt gtgtagttgt
    36841 tccggcaaac tcccgagata cgggaagccc ggttgaaaac ctaaaaacca gaccacgtat
    36901 tccacggagg agtgcaattc aacaacctgt ttttcgctaa acccgcaatg cgccgcgacc
    36961 accgccaaat ccggtccgcc tgcaccaccg taaaccaccg gaatttcaat aaagcgagac
    37021 tcaggttcca gggcctcgct ctcctcccac cagcgttgca aacgctctat ggcatccagc
    37081 gccagcgact caggattacg caaaatcacc gtgatattgt tcatgccggg aatggcttca
    37141 accacattcg gcatatccac cagacgctgc gccagtcgcc agatccgttt ctggctagcc
    37201 agcgtcaccg gcggttccag ttccagcact accgccgttt cacctatcag ataacaacgc
    37261 gctcgttgca ctttcgatcc ctctgattat tcgttatgca ggattaggaa tatcaataaa
    37321 ggtcacatca agatcggtat tttcattcag ccactcgctc aatgcgcgaa taccaccacg
    37381 ttcagtggca tggtgacctg cagcataaaa atgcaacccc tgctcgcggg ctgaatgaat
    37441 ggtctgttca gaaacttcgc cagtaataaa agcatccacg ccaaaacgcg cggcgctatc
    37501 gataaaactt tgcccgccgc ccgtgcacca ggcgacgcgc tggaccacct caggtccggt
    37561 atcgccacac cataacggct tacgtcccag acgcgcttca atccaggagg ctagttccag
    37621 tcccggcaca ggcatggtca gctctcccca cggcaccaac ggctcaattt cgcccatgac
    37681 cgtgatcccc agtaacgccg ccagctgtgc gttattgccc agctcaggat gtacgtcaag
    37741 cggcaaatgc cagccataca ggttgatatc attcgccagc aacgttttta aacggttacg
    37801 cttcattccg cgaatgaccg gagactctcc tttccagaag tagccatgat gcacaatgac
    37861 cgcatccgcg cccagacgca ctgcctcatc gagcaaagcc tggctggcgg tgacaccggt
    37921 aacaattttt tgcaccgtct ctttgccttc tacctgcaaa ccattcggcg cgtaatcact
    37981 aatcgccgcg ctgttcagtt tttcgttaat cagttgttcc agttcggtgt ttttcatctt
    38041 cgcgccttcc gtattctgat agctacaccg ttaacatagc gttacctcgc ctgttcgtcg
    38101 atagccaatc ttttgacata ttaagaaaca aacccgacgc caaaagacgc aaattgttta
    38161 tatcatcacg cttcagctaa tgtatattta tgcaatcaga cagcatattg cataaataat
    38221 cagatctgta tcaaagtttt aactttaagg aaacacaagc acgccgcgca actattacgt
    38281 gacgttgcag tcaattgtgg aattattatg aataaacacg catcacaacc gcgcactatt
    38341 tactatgtcg ttgcgctgca aatctgggaa tattttagct tttacggcat gcgtgccctg
    38401 ctgattctct atctcaccaa tcaactaaaa tacaacgata ctcacgccta cgagttattt
    38461 agcgcctact gttcgctggt gtatgtcacg ccaatcctcg gtggcttttt ggcggataaa
    38521 gttctcggca atcgcatggc ggtgatgctg ggggcgttgt tgatggcgat cggtcatgtg
    38581 gtgctgggtg ccagtgagat ccatccgtca ttcctctatc tgtccctggc gattatcgtt
    38641 tgtggctatg gtctgtttaa atccaatgtc agttgcctgc tcggcgagct gtatgagcca
    38701 accgatccgc gtcgtgatgg cggtttctcg ctgatgtatg cagcgggtaa cgtggggtct
    38761 attatcgcac ctatcgcctg tggttacgcc caggaagagt acagctgggc gatgggcttt
    38821 ggcctggcgg cggtgggtat gatcgcgggt ctggtcattt tcttatgtgg caatcgtcat
    38881 ttcacacata cccgcggcgt taataaaaaa gtactgcgtg caacaaactt tctcctgcct
    38941 aactggggat ggctgctggt tctgctggtg gcaacgcctg cgctgattac cgtactgttc
    39001 tggaaagagt ggtcggtata cgccttaatt gtcgcgacta ttattggcct gggtgtactg
    39061 gcaaaaattt atcgcaaagc agaaaaccag aaacagcgta aggagctggg gctgattgtg
    39121 acgctcacct tcttcagtat gttgttctgg gccttcgcac aacagggcgg cagctcaatt
    39181 agcctttata tcgaccgctt cgttaaccgc gatatgtttg gttataccgt tccgactgct
    39241 atgttccagt cgattaatgc cttcgcagtt atgctatgcg gtgtgttcct ggcgtgggtg
    39301 gttaaagaaa gcgtcgcagg taatcgtacc gtgcgcatct ggggaaaatt tgctcttggc
    39361 cttggcctga tgagcgccgg attctgcatt ctgaccttaa gcgcccgctg gtccgcaatg
    39421 tatggtcact cttctctgcc actgatggtg ttaggcctcg cggtgatggg ctttgcggaa
    39481 ctgtttatcg acccggttgc catgtcgcaa attacgcgca ttgaaattcc gggcgtgacc
    39541 ggcgtattaa ccggcatcta catgctgctt tctggcgcga tcgcgaacta tctggcgggc
    39601 gtgattgccg atcagacatc gcaggcttcg tttgatgctt ccggggcgat caactactcc
    39661 atcaatgcat atattgaagt atttgatcaa attacctggg gcgcactggc gtgtgtagga
    39721 ctggtactga tgatttggct gtatcaggcg ctgaaattca gaaaccgcgc gctggcgctg
    39781 gagtcataat aggccagcac c
//
LOCUS       DAJVRF010000019        39457 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJVRF010000019 DAJVRF010000000
VERSION     DAJVRF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.019"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(224..1564)
                     /gene="fadL"
                     /locus_tag="OQN13_000930"
     CDS             complement(224..1564)
                     /gene="fadL"
                     /locus_tag="OQN13_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCS1614357.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            1936..2220
                     /locus_tag="OQN13_000931"
     CDS             1936..2220
                     /locus_tag="OQN13_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCS1614358.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2400..3710
                     /gene="fadI"
                     /locus_tag="OQN13_000932"
     CDS             2400..3710
                     /gene="fadI"
                     /locus_tag="OQN13_000932"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCS1614359.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLCDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            3710..5854
                     /gene="fadJ"
                     /locus_tag="OQN13_000933"
     CDS             3710..5854
                     /gene="fadJ"
                     /locus_tag="OQN13_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCS1614360.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            6057..6542
                     /gene="sixA"
                     /locus_tag="OQN13_000934"
     CDS             6057..6542
                     /gene="sixA"
                     /locus_tag="OQN13_000934"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCS1614361.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            7226..7789
                     /locus_tag="OQN13_000935"
     CDS             7226..7789
                     /locus_tag="OQN13_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1614362.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            7871..10513
                     /gene="yfcU"
                     /locus_tag="OQN13_000936"
     CDS             7871..10513
                     /gene="yfcU"
                     /locus_tag="OQN13_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCS1614363.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNIGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            10533..11284
                     /locus_tag="OQN13_000937"
                     /pseudo
     CDS             10533..11284
                     /locus_tag="OQN13_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            11259..11807
                     /locus_tag="OQN13_000938"
     CDS             11259..11807
                     /locus_tag="OQN13_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1614364.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            11804..12274
                     /locus_tag="OQN13_000939"
     CDS             11804..12274
                     /locus_tag="OQN13_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1614365.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            12271..12795
                     /locus_tag="OQN13_000940"
     CDS             12271..12795
                     /locus_tag="OQN13_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1614366.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            12782..13654
                     /locus_tag="OQN13_000941"
     CDS             12782..13654
                     /locus_tag="OQN13_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCS1614367.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(13776..14327)
                     /gene="smrB"
                     /locus_tag="OQN13_000942"
     CDS             complement(13776..14327)
                     /gene="smrB"
                     /locus_tag="OQN13_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCS1614368.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIPEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            14493..15425
                     /gene="prmB"
                     /locus_tag="OQN13_000943"
     CDS             14493..15425
                     /gene="prmB"
                     /locus_tag="OQN13_000943"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS1614369.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            15460..16545
                     /gene="aroC"
                     /locus_tag="OQN13_000944"
     CDS             15460..16545
                     /gene="aroC"
                     /locus_tag="OQN13_000944"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCS1614370.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            16549..17373
                     /gene="mepA"
                     /locus_tag="OQN13_000945"
     CDS             16549..17373
                     /gene="mepA"
                     /locus_tag="OQN13_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCS1614371.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            17373..18182
                     /locus_tag="OQN13_000946"
     CDS             17373..18182
                     /locus_tag="OQN13_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCS1614372.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            18182..18730
                     /gene="epmC"
                     /locus_tag="OQN13_000947"
     CDS             18182..18730
                     /gene="epmC"
                     /locus_tag="OQN13_000947"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCS1614373.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            18764..19042
                     /locus_tag="OQN13_000948"
     CDS             18764..19042
                     /locus_tag="OQN13_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCS1614374.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(19163..21169)
                     /gene="mnmC"
                     /locus_tag="OQN13_000949"
     CDS             complement(19163..21169)
                     /gene="mnmC"
                     /locus_tag="OQN13_000949"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCS1614375.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            21328..22548
                     /gene="fabB"
                     /locus_tag="OQN13_000950"
     CDS             21328..22548
                     /gene="fabB"
                     /locus_tag="OQN13_000950"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCS1614376.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            22823..24001
                     /locus_tag="OQN13_000951"
     CDS             22823..24001
                     /locus_tag="OQN13_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1614377.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(23998..24993)
                     /gene="flk"
                     /locus_tag="OQN13_000952"
     CDS             complement(23998..24993)
                     /gene="flk"
                     /locus_tag="OQN13_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCS1614378.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            25092..26228
                     /gene="pdxB"
                     /locus_tag="OQN13_000953"
     CDS             25092..26228
                     /gene="pdxB"
                     /locus_tag="OQN13_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCS1614379.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            26294..27307
                     /locus_tag="OQN13_000954"
     CDS             26294..27307
                     /locus_tag="OQN13_000954"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS1614380.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            27307..28119
                     /gene="truA"
                     /locus_tag="OQN13_000955"
     CDS             27307..28119
                     /gene="truA"
                     /locus_tag="OQN13_000955"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCS1614381.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            28202..28861
                     /locus_tag="OQN13_000956"
     CDS             28202..28861
                     /locus_tag="OQN13_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1614382.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            29017..29931
                     /gene="accD"
                     /locus_tag="OQN13_000957"
     CDS             29017..29931
                     /gene="accD"
                     /locus_tag="OQN13_000957"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCS1614383.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            30001..31269
                     /gene="folC"
                     /locus_tag="OQN13_000958"
     CDS             30001..31269
                     /gene="folC"
                     /locus_tag="OQN13_000958"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCS1614384.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            31259..31921
                     /gene="dedD"
                     /locus_tag="OQN13_000959"
     CDS             31259..31921
                     /gene="dedD"
                     /locus_tag="OQN13_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCS1614385.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            32180..32668
                     /gene="cvpA"
                     /locus_tag="OQN13_000960"
     CDS             32180..32668
                     /gene="cvpA"
                     /locus_tag="OQN13_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCS1614386.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            32705..34222
                     /gene="purF"
                     /locus_tag="OQN13_000961"
     CDS             32705..34222
                     /gene="purF"
                     /locus_tag="OQN13_000961"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCS1614387.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            34317..34886
                     /gene="ubiX"
                     /locus_tag="OQN13_000962"
     CDS             34317..34886
                     /gene="ubiX"
                     /locus_tag="OQN13_000962"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCS1614388.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            35152..35934
                     /gene="argT"
                     /locus_tag="OQN13_000963"
     CDS             35152..35934
                     /gene="argT"
                     /locus_tag="OQN13_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCS1614389.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            36155..36937
                     /gene="hisJ"
                     /locus_tag="OQN13_000964"
     CDS             36155..36937
                     /gene="hisJ"
                     /locus_tag="OQN13_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCS1614390.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            37027..37713
                     /gene="hisQ"
                     /locus_tag="OQN13_000965"
     CDS             37027..37713
                     /gene="hisQ"
                     /locus_tag="OQN13_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCS1614391.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            37710..38426
                     /locus_tag="OQN13_000966"
     CDS             37710..38426
                     /locus_tag="OQN13_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1614392.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            38434..39207
                     /gene="hisP"
                     /locus_tag="OQN13_000967"
     CDS             38434..39207
                     /gene="hisP"
                     /locus_tag="OQN13_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCS1614393.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
BASE COUNT         9344 a         9666 c        10608 g         9839 t
ORIGIN      
        1 aatgcagaaa agtaaactct ttgagaagga cgagcagaac gcctgaaaat atgcctttcc
       61 cttcaggttt gtctgtggat accgcttatt gatttgaaag ggcaagctgc caacaggctg
      121 tttgcaaaac tcaggaggta agcaatgagg acactacttt cggtgaagtg ggggagttaa
      181 agtcacctgc tatgcaggtg actttatcca ggcgaacgcg ttatcagaac gcgtagttaa
      241 agttagtacc gaacagccag gctttacctt cagactcgaa ctggtatggg ccttcgttaa
      301 ttttcacgct ctgaccgtgc atataagaaa caccaacgtc gactgaagca tctttattaa
      361 atgcgtaagt cgtacctgca ctcagccaga aacggtcctg gtccggaatg gagatagaac
      421 gattctgagc cggaactggg ctgtcatcaa aggcgatacc ggtacggaag gtccagttat
      481 catcgtagta ataagtggta cccaacgcga tgcggtaagc atctttaaag ccttcatgtt
      541 tctggaacag cgtgtcgcca ctggttgagg tcgctttcag ctgctggaac tgactccagc
      601 tggtgtaagc caggctatag tgaatcgccc actgtggatc aacacggtta taacctgaca
      661 cttcccacat ttcaggcagg ttcagcgtca gataacccga ttgcgttgcg ccacctgtcg
      721 cggtaggaat tggcaaaccg tagttattaa acgcacgatt aagatcgctg ctgtagttac
      781 ctttgaagtc aattttcact tcagaacggt aggtcagtgc atagcggtta tttttatcca
      841 gttcatacag gattccggcg ttccagccaa agccccactg gttaccgttc agatgagcga
      901 ttttggtatt actgtcgata ccgttggcgg tagctgccaa tgcttgccct tgaggagttt
      961 gcccggcagg agattgcata atctgaccag caaccaactg ccccagatcg cctgcgaaac
     1021 gttcaatttt cgcgcgagcg tagacggcgt tgaaaccaag accaaagctc catgcattat
     1081 ttaagcgata cgcaccgctt aagttcaggt tcatggtttc aaggtcagtt gtacccccga
     1141 cagagccgcc tgcataagta tcattaaact ctgttgccag accatagtta gaggtaatag
     1201 aagcgcccca accaaattgg tcgttaatcg gtgcaacaaa gtgcatgttc ggaacccatg
     1261 ccgtaggcgc gatgttatcg gctttcaggc tacgaccaga cggagacgtt ccgctgatat
     1321 tcacatccgg gtcaatataa accgcacccg cagaaaatgt cgggcggtca aacatggtaa
     1381 tcaatgcggg gttacggcta acgttacctg catcatcggc aattgcgcct tcccctgaat
     1441 aagcccggcc caggccagag gaagaaaatt cgtttaactg aaagcctgcc gaccaggcct
     1501 gggtggagat aagtgccact gcgactgcga gagcagactt tgtaaacagg gttttctggc
     1561 tcatgaccat aacctcaatg atttattttt atacaaacta tgttacgtgc tgtaacagga
     1621 gcgcgaagtg tagggtctga ggtacatctt agaaatcaga ccagtggcga gagtataggt
     1681 cggaccagct ggaatgctgc aagtatgttt ccatcttttt tcaaatatga tctaagaaac
     1741 gcgatccagc tggcaaaatt tggaggttgc gcactcgcgg attcgggtga attttgtttt
     1801 agatcatttt taagtgtgat ttcggtcact tatccgattt tgcagaatta acaactggag
     1861 cagcactttc cggaagcgta aaatatccac aacgtttatc tggcaccttg ggtgcacata
     1921 cagaggaaat caacgatgag taaatgtagt gctgatgaaa ccccggtttg ctgctgtatg
     1981 gatgttggca ccattatgga caactccgac tgcaccgcgt cttacagccg cgtgtttgca
     2041 aaccgcgcag aagcagaaca aacgctggcg gcgctgactg aaaaagcccg tagcgtggaa
     2101 tccgaaccgt gcaaaatctc cccgactttc actgaagagt ctgacggtgt gcgcctggat
     2161 atcgatttca cttttgcctg cgaagctgaa atgctgatct tccagctggg tctgcgttaa
     2221 ttcctctcgg gtacgttcgc ccctgtcagt tgctggcagg ggcgttttct tttctttctc
     2281 ctgtcttcac tgtgtcatag ctcccacttt cctttttctc cgcttggcta aatgtaaaaa
     2341 aatagttaag actatgatca ggtcagacca ctttatttat ttttttacag gggagtgtta
     2401 tgggtcaggt tttaccgctg gttacccgcc agggcgatcg tatcgccatt gttagcggtt
     2461 tacgtacgcc ttttgcccgc caggcgacgg cttttcatgg cattcccgcg gttgatttag
     2521 ggaagatggt ggtaggcgaa ctgctggcac gcagcgagat ccccgctgaa gtgattgaac
     2581 agctggtctt tggtcaggtc gtacaaatgc ctgaagcccc caacattgcg cgtgaaattg
     2641 ttctcggtac cggaatgaat gtgcataccg atgcttacag cgtcagccgc gcttgcgcta
     2701 ccagtttcca ggcggttgca aacgtcgcag aaagcctgat ggcgggaact atccgagcgg
     2761 ggattgccgg tggggcagat tcctcttccg tattgccaat tggcgtcagt aaaaaactgg
     2821 cgcgcgtgct ggttgatgtc aacaaagctc gaaccatgag ccagcgcctg aaactcttct
     2881 ctcgcctgcg tttgtgcgac ttaatgcccg taccgcctgc ggtagcagaa tattctactg
     2941 gcttgcggat gggcgacacc gccgaacaaa tggcgaaaac ctacggcatc acccgagaac
     3001 agcaagatgc attagcgcac cgttcgcatc agcgtgccgc tcaggcatgg tcagacggaa
     3061 aactcaaaga agaggtgatg actgccttta tccctcctta taaacaaccg cttgtcgaag
     3121 acaacaatat tcgcggtaat tcctcgcttg ccgattacgc aaagctgcgc ccggcgtttg
     3181 atcgcaaaca cggaacggta acggcggcaa acagtacgcc gctgaccgat ggcgcggcag
     3241 cggtgatcct gatgactgaa tcccgggcga aagaattagg gctggtgccg ctggggtatc
     3301 tgcgcagcta cgcatttact gcgattgatg tctggcagga catgttgctc ggtccagcct
     3361 ggtcaacacc gctggcgctg gaacgtgccg gtttgacgat gagcgaactg acattgatcg
     3421 atatgcacga agcctttgca gctcagacac tggcgaatat tcagttgctg ggtagtgaac
     3481 gttttgctcg tgaagtactg gggcgtgcac atgccactgg cgaagtggac gatagcaaat
     3541 ttaacgtgct tggcggttcg attgcttacg ggcatccctt cgcggcgacc ggcgcgcgga
     3601 tgattaccca gacattgcat gaacttcgcc gtcgcggcgg tggatttggt ttagttaccg
     3661 cctgtgctgc cggtgggctt ggcgcggcaa tggttctgga ggcggaataa tggaaatggc
     3721 atcagcgttt actcttaatg ttcgtctgga caacattgct atcatcacca tcgatgtacc
     3781 ggatgagaaa atgaataccc tgaaggcgga atttgcctcg caggtgcgcg ccattattaa
     3841 gcaactccgt gaaaacaaag agttgcgcgg cgtggtgttt atctccgcga aaccggacaa
     3901 ttttatcgcc ggagcagaca tcaacatgat tggcaactgc aaaacggcgc aagaagcgga
     3961 agcactggcg cggcagggcc aacagttgat ggcggagatt catgctttgc ccattccggt
     4021 tatcgcggct attcatggcg cttgcctggg cggtgggctg gagctggcgc tggcgtgcca
     4081 cggtcgcgtt tgtactgacg atcctaaaac ggtgctcggt ttgcctgaag tacaacttgg
     4141 attgttaccc ggttcaggcg gcacccagcg tttaccgcgt ctgataggcg tcagtacagc
     4201 attagagatg attctcaccg gaaaacaact tcgggcgaaa caggcattaa agctggggct
     4261 ggtggatgac gttgttccgc actccatttt gctggaagcc gctgttgagc tggcgaagaa
     4321 ggatcgccca tcttcccgcc ctctacctgt acgcgagcgt attctggcgg ggccgttagg
     4381 tcgtgcgctg ctgttcaaaa tggtcggcaa gaaaacagaa cacaaaactc aaggcaatta
     4441 tccggcgaca gaacgcatcc tggaggttgt tgaaacggga ttagcgcagg gcaccagcag
     4501 cggctatgac gctgaagctc gcgcctttgg cgaactggcg atgacgccgc aatcgcaagc
     4561 tttgcgtagt atcttttttg ccagtacgga cgtgaagaaa gatcccggta gtgatgcacc
     4621 gcctgcgcca ttaaacagcg tgggaatttt aggtggtggc ttgatgggcg gcggtattgc
     4681 ttatgtcact gcttgtaaag cggggcttcc ggtcagaatt aaagatatca acccgcaggg
     4741 cataaatcat gcgctgaagt acagttggga tcagctggag ggcaaagttc gccgtcgtca
     4801 tctcaaagcc agcgaacgtg acaaacagct ggcattaatc tccggaacga cggactatcg
     4861 cggctttgcc catcgcgatc tgattattga agcggtgttt gaaaatctcg aattaaaaca
     4921 acagatggtg gcggaagtgg agcaaaattg cgccgctcat accatttttg cttcgaacac
     4981 gtcatcttta ccgattggtg atatcgccgc tcacgccgcg cgacctgagc aagttattgg
     5041 cctgcatttc ttcagtcctg tggaaaaaat gccgctggta gagattattc ctcatgcggg
     5101 tacatcggcg caaaccatcg ccactacggt aaaactggcg aaaaaacagg gcaaaacgcc
     5161 cattgtcgtg cgtgacaaag ccgggtttta cgtcaatcgc atcttagcgc cttacattaa
     5221 tgaagctatc cgcatgctga ccgaaggtga acgggtagag cacattgatg ccgcgctagt
     5281 gaaatttggt tttccggtag gcccaatcca acttttggat gaggtaggaa tcgacaccgg
     5341 gactaaaatt attcctgtac tggaagccgc ttatggagaa cgttttagcg cgcctgcaaa
     5401 tgttgtttct tcaattttga acgacgatcg caaaggcaga aaaaatggtc ggggtttcta
     5461 tctttatggt cagaaagggc gtaaaagcaa aaaacaggtc gatcccgcca tttacccgct
     5521 cattggcgca caagggcagg ggcgactctc cgcaccgcag gttgctgaac ggtgtgtgat
     5581 gttgatgctg aatgaagcag tacgttgtgt tgatgagcag gttatccgta gcgtgcgtga
     5641 cggggatatt ggcgcggtat ttggcattgg ttttccgcca tttctcggtg gaccgttccg
     5701 ctatatcgat tctctcggcg cgggcgaagt ggttgcaata atgcaacgac tggccacgca
     5761 gtatggttcc cgttttaccc cttgcgagcg tttggtcgag atgggcgcgc gtggggaaag
     5821 tttttggaaa acaactgcaa ctgaccttca ataagaaggt caaagctata tgaatccgcg
     5881 ctgaatggcg gagtgttggt caaaatgtaa acgcatattg actatactta cgccattgag
     5941 gtaaaaaaca gcgtttcatt cggtgaatgg ataaggcaca atgccggcca ccgttttctt
     6001 tctctggttt cagatgaaag aaaacggacg aatctggtta acaaaagcgg tgcaatatgc
     6061 aagtttttat catgcgtcac ggcgacgcag cccttgatgc cgccagtgat tcggttcgtc
     6121 ctctgaccac taatggttgt gacgaatctc gcctgatggc gaactggctg aaaggtcaaa
     6181 aagtggaaat cgaacgcgtt ctggtgagcc cgttcctgcg agccgagcaa acactggaag
     6241 aagtagggga ttgtctgaac ctgccttcca gtgcagaagt tctgccggag ttaacgccct
     6301 gcggcgatgt tggtctggtc agtgcctatt tgcaggcgtt gaccaatgaa ggtgttgctt
     6361 cggtactggt gatctctcac ttaccgttag tcgggtatct ggttgccgag ttatgtccgg
     6421 gcgaaacgcc gcctatgttt accacctcgg ccattgccag cgtaacgctt gatgaaagcg
     6481 gaaacggcac attcaactgg cagatgagcc cgtgtaatct gaagatggca aaagctatct
     6541 gatgagttcc aaattatgcc caatgaggta gattcattgg gcataattgc tcatcaggca
     6601 gcggatatct gtcaatctca ctactatcga agactctccc catctataaa taaaacactt
     6661 tctactttca ttcggaaatt attcttctgc gcgtatcttg attttgccca aagttacatg
     6721 ttgaaatgaa taatgtctgt ttgacactca ttgtcattta ttaatacaga taatagagaa
     6781 aatagagttg aataatcagg caggatgtaa ttgaaacttg attaaattct ggctatagtg
     6841 tttatatatt atattgtcaa aagtggataa atgccaatcg cattatatgt tcattgtttg
     6901 ttttttaagc tgttgatatt cattattgat atactttcta ttttttctaa atacttattc
     6961 cgtatttttt aaggatgtcc ttaagataac ctttcttctg tcaatatatt aattaaatct
     7021 acatttactc tttagtaaaa ttaaattgca tcaggggtgt ggaaacatca ctatgcggcc
     7081 ccacaccaac gaatacaatt ttctacgacc tgttcttttt ggatcgcatg ccgtattcaa
     7141 tggctgggta taaacattgg ttattgactg aatcaatgta ttgcaatgtt tatattttta
     7201 attatttata gtttaaggac aaataatgag taagtttgtt aaaacagcta ttgctgcggc
     7261 aatggtgatg ggcgtgttca cctctacggc aacaatcgct gcgggtaaca acggtactgc
     7321 acgtttttac ggcaccattg aagactcagt gtgttctatc gttccggacg atcacaaact
     7381 ggaagttgat atgggtgata tcggtgccga aaaactgaaa aataacggca ccaccacgcc
     7441 gaaaagtttc cagattcgtc tgcaagattg cgtatttgat actcaggaaa caatgaccac
     7501 tacctttact ggtaccgttt cttctgcaaa tagcggcaat tattacacca ttttcaatac
     7561 cgatacctgt gcggcattta acaatgttag cctggcgatc ggtgactctc tgggtacctc
     7621 ttacaaaagc ggcatgggta ttgaccagaa aatagtgaaa gacacttcta ccaataaagg
     7681 caaagcgaag cagacactga actttaaagc ctggctggtc ggcgcagctg atgcgccaga
     7741 tctgggtaat ttcgaagcaa ataccacctt ccagattact tacctgtaag gcataccccc
     7801 atagcgacac tgctatgggg gatttaaatg gattttgacc gaacaagaat aaaaacggat
     7861 agaaacgtgt atgcctgacc attctctttt tcgattacgg attcttccgt ggtgcattgc
     7921 gctggcaatg tcagggagtt atagcagtgt ctgggctgaa gacgacattc agtttgattc
     7981 ccgttttctg gaattaaaag gcgacacaaa aattgatctg aagcgttttt ccagccaggg
     8041 atatgttgag cccggaaaat acaatttaca ggttcaacta aataaacagc cattggcgga
     8101 agagtacgat atttactggt atgctggtga agatgacgcg agcaaaagct atgcttgtct
     8161 gacaccggaa ctggtagcgc agtttggttt aaaagaagac gtggcgaaaa atctgcaatg
     8221 gagccacgat gctaaatgcc tgaaatccgg tcaactggaa ggcatggaaa ttaaggctga
     8281 tttaagccag tccgcattag tcatttcact gccacaggct tacctcgaat atacttatcc
     8341 cgactgggat ccgccttcac gttgggatga cggcatctcc gggatcgtcg cggactacag
     8401 catcaacgca caaacccggc acgaagaaaa tggcggtgat gatagtaacg agatcagcgg
     8461 caacgggacg gtcggggtta acctggggcc gtggcgtatg cgtgctgact ggcagactaa
     8521 ctatcaacat actcgcagta atgatgacga tgaattcagc ggcgatgaaa ctcaaaaaaa
     8581 atgggagtgg agtcgctact atgcctggcg ggcgttacca tcattaaaag ccaaactggc
     8641 gctgggcgag gattacctca gatccgatat ttttgatggt tttaactatg ttggtggcag
     8701 tgtcagtact gacgatcaaa tgttgcctcc caatctgcgc ggctacgcgc cagacatttc
     8761 cggcgtggca cacaccacag caaaagtgac cgtcagccag atggggcgtg tgatttacga
     8821 aacgcaggtt ccggctggac cgtttcgtat tcaggatctt ggtgattctg tctccggtac
     8881 gttgcatatt cgcattgaag aacagaacgg ccaggtgcag gaatatgaca tcagcaccgc
     8941 ctcgatgcca tacctcactc gtccaggtca ggttcgctat aagatcatga tgggccgtcc
     9001 gcaagagtgg ggacaccatg tcgagggtga atttttttct ggtgctgaag cttcctgggg
     9061 gatcgctaac ggctggtcgt tatatggcgg cgcactggga gatgaaaact atcagtctgc
     9121 ggcgcttggc gtcggtcgcg atttgtctac attcggcgcg gtcgcgtttg atgttactca
     9181 ctcgcacacc aaactggata aagacaccgc ttatggcaaa ggttcgctgg acggtaactc
     9241 cttccgtgtg agttattcca aagactttga ccagctcaac agccgcgtta cttttgctgg
     9301 atatcgcttc tcggaagaga actttatgac catgagcgag tatctggatg ccagtgacag
     9361 cggaatggta cgcacgggca acgacaaaga gatgtacact gccacttata accagaactt
     9421 ccgcgatgcg ggtgtttcgg tttatctcaa ctatacccgc catacctact gggatcgcga
     9481 ggagcagaca aactacaaca tcatgctctc gcactatttc aatataggca gtattcgtaa
     9541 tgtcagcatc tcgatgactg gctaccgtta cgagtatgac aaccaggccg acaaaggcat
     9601 gtacatttcg ctcagtatgc cgtggggtga caacagtacc gttagctata acggtaacta
     9661 tggcagtggg acggacagca gtcaggtcgg ttatttcagc cgtgtcgatg acgcgactca
     9721 ctatcagttg aacgtcggca ccagtgacaa acacaccagc gttgacggct attacagcca
     9781 tgatggttcg ctggcgcagg ttgacctcag tgcgaactac catgaagggc aatacacctc
     9841 tgcgggcttg tcgttacagg gcggcgcaac gcttactgcc cacggtggcg cacttcaccg
     9901 tacccagaat atgggcggga cacgcttgtt gattgatgcc gatggcgttg ccgatgttcc
     9961 ggtagaaggt aacggtgctg ctgtttatac caatatgttt ggtaaagccg tcgtttctga
    10021 cgtcaataac tattaccgca atcaggcgta tatcgacctc aacaaactgc cggaaaacgc
    10081 cgaagcaacc cagtcggtgg tacaagccac gctaactgaa ggggcgattg gctaccgcaa
    10141 atttgccgtc atcagtggtc aaaaagcgat ggcggtgctg cgtttacaag acggcagcca
    10201 tcctccgttt ggcgcagaag taaaaaatga taacgagcag acagtgggcc ttgtcgatga
    10261 tgacggcaat gtttatctgg ctggggtgaa acctggcgaa cacatgagtg tgttctggag
    10321 tggtgttgcg cattgcgata tcaacctgcc ggacccgctg cctgccgatc tgtttaacgg
    10381 cttgttactg ccatgccagc ataaaggcaa cgtcgcgccg gttgttcctg atgacataaa
    10441 gcctgtcatt caggagcaga cgcaacaggt gacacccacc aatccccctg tttctgtttc
    10501 agctaaccaa taacgtgatt aaggaatgat ccatgtctga tttactttgc tctgccaaat
    10561 tgggtgctac gacgctagcg ttactgctta gcgctgccag tttgtcgccc aggcttctgt
    10621 aacgccagat cgtacccgtt tgatatttaa tgaaagcgac aaatcaatca gcgtgacgtt
    10681 gcgcaataac gatccgaaac tgccttatct ggcccaaagc tggatcgaag atgaaaaggg
    10741 taacaagatc agttcaccgt taactgttct gccaccggtg caacgcatcg attcaatgat
    10801 gaacgggcag gtaaaagtgc agggaatgcc ggatatcaac aaattgcctg ccgatcgtga
    10861 aagcctgttc tacttcaacg tgcgtgagat cccgccaaaa tcaaacaaag ccaacacgct
    10921 gcaaatagca ctacaaacgc gtattaagtt gttctggcga ccgaaggcgc tggagaacgt
    10981 cagcatgaaa aacccctggc aatataaggt tactttaacc cgcaacggtc aggaatttac
    11041 cgtcaataac ccgacgccgt attacgtcat tttcagtaat gccagcacac agaaaaatgg
    11101 caatcctgca gccggtttct cgccaatagt gatgtcgccg aaaacctctg caccgctgaa
    11161 tgtgaagatg ggtagcgtgc cggtactgac ctatgtcaac gactatggcg ctcggatgcc
    11221 gctctttttc acttgcaacg gtaacagttg ccaggtcgat gaagagcaga gccgcaaagg
    11281 ctgaggccgg gagagccagt atgaaaggat cttgttctgt ctgggcggta actgggttac
    11341 tgatatgttg tgttggtagc ggctttgcag ttgcagcgag taagtcagta agtatgaatt
    11401 tacgggtgct tgtcgatgcc cctcctcctt gtacggtaaa tggtgcggca gttgagtttg
    11461 gtaatgtatt cattaataag atcaatgggg ttgattataa acgtcccatt gactactcgc
    11521 ttgtttgtaa caatctggca atggatgacc tgcgtctaca gatgcaggcg acaacggtcg
    11581 tgattaacgg tgaaacagtt atcagcacgg gtattcctgg gtttggtatt cgggttcaga
    11641 aaagtagtga tcacacaata ctcgacttaa cctctggctc atggttgcca tttaattttt
    11701 cctcgggagt tcccgttctg gaagctgtgc cggtgaaaca gagtggtaca acgttagcgg
    11761 cagctgaatt taacgccagt gccacaattg tggtggatta ccaatgaaac ggatctcgct
    11821 tattttgctg tggggctttt gctcgatggc actctcaaac gtctcttttc acggttattt
    11881 agtgcagccg cctaactgca cgattagcaa cgctcaaacc attgaaataa cttttcagga
    11941 tgtgcttatt gatgacatta atggcagcaa ttatgagcaa accgtgccgt attccattac
    12001 ctgcgatacg gcggttcgcg acccattaat ggaaatgacc ttgagctgga gtggcacact
    12061 aagcgatttt gataatgctg cagtaagcag caatatcact ggtttaggca ttcagctcaa
    12121 acaggcaggg cagagtttta cgatcaatac gccacttatg gtgaatgaaa ccgatttacc
    12181 ggtgttaacg gctgtgccag taaaaaagag tggtgtcgtt ctgccagagg cggactttga
    12241 ggcctgggcc acgttacagg tagattacca atgaagaaaa agcgtacgtt gttttttatt
    12301 agtagcctga tgttgctggg cagtggaacc acaatagcag gagataattt gcactttacc
    12361 ggcaatttaa tcagtaaatc gtgtacgcca gtgattaatg gtagccagtt ggcagaagta
    12421 cattttcctg ccattgccgc cagcgattta atgaaccttg ggcagtcaga gcgagtgcca
    12481 ttggtgtttc aattgaaaga ttgtcatagt tctacattgt ttaatgtcaa agtaacgttg
    12541 acaggaacag aagatagtgc tctacccggc tttctggcct ttgactcctc gtcatcagca
    12601 tccggcgctg ggattggtat tgaaactgcg gcaggcacat cggtgccaat caacaataca
    12661 acaggcgtca cgctcccgct gaatcaggga aataacagcc taaattttaa tacctggtta
    12721 caggcaaaga gcgggcgaga tgtaacgtcc ggagacttta gtgccacagt gacggcaacc
    12781 tttgagtatt tctgaaatga aaaaatggac cataattttc gcgagcctaa tgctgctcgt
    12841 tctgagtgtt gtcggcgcat ctaagagtta tgctggagat aaacttatga gtgcgagttt
    12901 tgacagtgtg agagtttatt atgccatgga caaggtaacc ggtgctgtag cgagcagtgt
    12961 tttcaatgtt acggtgataa cgccaaagga ggttacatat gggaaatatg attcttttgc
    13021 gtataaaggc gatacgttgc gggttatttc ctggtctggg tcgggttcag ctccaacttt
    13081 ggtactgact gactttgata caattaataa ctcaaattgc cctggcatag atacgaaaat
    13141 ttttcgttgt gcatatatga cgtttaaaat taccgttgca tctgatgatt atggttgccc
    13201 ttggatcgca tccttttatt cctatacaga tttacccgga tttggaagct atacggcccc
    13261 tacagtgcat aacacaatat gcccaacgat accggtcact tcatacgata tctcctggag
    13321 tgaaaattat gtcagccata ataaggcgtt acgaattcag tccacggggt caacagtgac
    13381 caccacgctt tcgacctacc tgatggaagg cggcagactc tgtgatggca gtaatttttc
    13441 tgataatgat ggcagaggag catactgccg cgcggtatca gaattgttaa cctttacctc
    13501 ttacggttgt gataaatcaa cggtaacggt tacgccaact cgtcatccgg taacggataa
    13561 agtactacac gatattgtgg tgaatgtgaa taccagtagt gggcaaccga ttgattcgac
    13621 gtgtcgtttt cagtatgtat tgaatgaact gtagttcgta gtattgctct gatgcgtctt
    13681 atcaggccta cgaagtgaat tgcaatttat tgaattatca catatttgta ggccggataa
    13741 ggcgtttacg ctgctccggc atttgctgcc agcattcagg gcaactccgg cggcaaccac
    13801 tcttccactt caatcaacac caacaaagcc gcatcaccgc cgtactcttt cggtgcctgg
    13861 tgaaatgcca tcacatgcgg atgttgtgcc agccacagcg gtgtttgttg cttcaaaata
    13921 tgcttcccgt gcccatgcat cacgcaggca caaaacacat gttcgcggcg acaggcggca
    13981 atcaacgcgc ccagttcctg cttggcctgc agctgcgtca gaccgtgtaa atccaaaaac
    14041 aattccggtg aataatcgcc gcggcgcagt ttcttcgcct caaaatggct gacatccggg
    14101 cgaacatatt tcaccggacc ttcggtattt aataacggct gaaactcatc ggagaaataa
    14161 tggctggcat cagcctgctc cgggatcaag cgtttcaccg gcacttcgct gattttttta
    14221 cgctgcggtc ggtggacaat cgtgtcctgc ttaatcttgc gagtccccgc catcaactgg
    14281 cgaaacagag cctggtcctc ctcgctaagt gttgttttct ttttcattta tctgtctcta
    14341 tttctaattt tcctacagtt tacccgactc gtagcacaat cgatcgtttt aaacgtattt
    14401 ttcctcactc accgcgcgtg aatgctgatt tatcgtcatc ttcatggcaa actagccgcc
    14461 gaaattattg cgatcatgcc ctggaggaat acgtggataa aattttcgtt gatgaagcag
    14521 taaatgagct gcaaaccatt caggacatgt tgcgctggtc ggtaagccgc ttcagcgcgg
    14581 caaatatctg gtacggtcac ggcaccgata acccgtggga tgaagccgta cagctggtgt
    14641 taccatcgct ctacctgccg ctggatatcc cggaagatat gcgcaccgcg cgtctgacct
    14701 ccagcgaaaa acaccgtatt gttgaacgcg tgatccgccg cgtcaacgag cgcattccgg
    14761 tggcttacct gaccaacaaa gcctggttct gcggtcatga attttacgtc gatgaacgcg
    14821 tgctggtgcc gcgctcgccg attggtgaac tgatcaacaa taaatttgcc ggactcatta
    14881 gcaagcaacc gcagcatatt ttagatatgt gtaccggtag cggctgcatc gccattgcct
    14941 gtgcttatgc cttcccggaa gcggaagtcg acgcggtaga tatctctccc gacgcgctgg
    15001 cggttgccga acagaacatc gaagaacacg gtctgatcca caacgtcatt ccgattcgtt
    15061 ccgatctgtt ccgtgatttg ccgaaagtgc agtacgatct gattgtcact aacccgccgt
    15121 atgtcgacgc ggaagatatg tccgacctgc caaacgaata ccgtcacgag ccggaactgg
    15181 gcctggcatc tggcaccgac ggccttaaac tgacgcgtcg cattcttgga aacgcggcag
    15241 attaccttgc tgatgatggc gtgttgattt gtgaagtcgg caacagcatg gtacatctta
    15301 tggaacaata tccggatgtt ccgttcacct ggctggagtt tgataacggc ggcgatggtg
    15361 tgtttatgct caccaaagag cagctgctgg cggcccgtga atatttcgcg atttataaag
    15421 attaagtaaa cacgcaaaca caacaataac ggagccgtga tggctggaaa cacaattgga
    15481 caactctttc gcgtaaccac cttcggcgaa tcgcatgggc tggcgctcgg ctgcatcgtc
    15541 gatggtgttc cgccaggcat tccgctgacg gaagcggacc tgcaacatga cctcgaccgt
    15601 cgtcgccctg ggacatcgcg ctataccacc cagcgccgcg agccggatca ggtcaaaatt
    15661 ctctccggtg tttttgaagg cgttactact ggcaccagca ttggcttgtt gatcgaaaac
    15721 actgaccagc gctctcagga ttacagcgca attaaagacg ttttccgccc aggccatgct
    15781 gattacacct acgaacaaaa atacggtctg cgcgattatc gcggcggtgg acgttcttcc
    15841 gcccgcgaaa ccgccatgcg cgtggcggca ggggcgattg ccaaaaaata tctcgccgag
    15901 aaatttggta ttgaaatccg cggctgcttg acccagatgg gcgacattcc gctggaaatc
    15961 aaagactggt cgctggttga acaaaatccg ttcttctgcc cggacccgga caaaatcgac
    16021 gcgttagatg aactgatgcg cgcgctgaaa aaagagggcg actccatcgg cgcgaaagtc
    16081 accgttgttg ccagtggtgt ccccgccgga cttggcgagc cggtctttga ccgcctggat
    16141 gccgacatcg cccatgcgct gatgagcatc aacgcggtga aaggcgtgga aattggcgac
    16201 ggttttgacg tagtggcgct gcgcggcagc cagaaccgcg acgaaatcac caaagacggt
    16261 ttccagagca accatgcggg cggcattctc ggcggtatca gcagcgggca gcaaatcatt
    16321 gctcatatgg cgctgaaacc gacctccagc attaccgtgc cggggcgcac tattaaccgc
    16381 tttggcgaag aagttgagat gatcaccaaa ggccgtcacg atccctgtgt cgggatccgt
    16441 gcagtgccga tcgctgaagc gatgctggcg atcgttttaa tggatcacct gttacggcag
    16501 cgggcgcaaa atgccgatgt gaagactgat attccacgct ggtaaaaaat gaacaaaacc
    16561 gcgattgcgc tgctggctct gcttgccagt agcgctagcc tggcagcgac gccgtggcaa
    16621 aaaataaccc aacctgtgcc gggtagcgca caatcgatag gcagtttttc taatggctgt
    16681 attgtcggcg ctgacacgtt gccgatacag tccgaacatt atcaggtcat gcgtaccgat
    16741 cagcgtcgct atttcggtca cccggatctg gtgatgttta tccagcgtct gagtagccag
    16801 gtgagcaatc tgggcatggg tacggtgctg attggcgata tggggatgcc cgctggtggg
    16861 cgtttcaacg gcggtcatgc cagccaccag accggactgg atgtcgatat ctttctgcaa
    16921 ctgccgaaaa ctcgctggac ctccgcgcag ctcttgcgcc cgcaagcact ggacttagtt
    16981 tcccgcgacg gtaaacacgt tgtctccacg ctgtggaagc cagaaatttt cagcttgatc
    17041 aaactcgctg cccaggacaa agacgtcacg cgcatttttg ttaatccggc gattaaacaa
    17101 caactttgcc ttgatgcggg caccgatcgc gactggttgc gcaaagtgcg accctggttc
    17161 cagcatcgcg cgcatatgca tgtacgatta cgttgccctg ccgatagtct ggagtgtgaa
    17221 gatcaacctt taccgccatc aggcgatggt tgcggggcag aactgcaaag ctggtttgca
    17281 cctctaaaac cgggaacaac aaagcctgag aagaagacac cgcctccgtt gccgccttcc
    17341 tgccaggcgc tactggatga gcacgtgatc taatggaaac gtttaatagc ctgtttatgg
    17401 tttccccgct gttgctggga gttctctttt ttgtcgccat gctggcggga tttatcgact
    17461 cgattgccgg tggcggtggg ttactcacca ttccggcatt gatggctgcg ggaatgtctc
    17521 ccgctaacgc gctggcaacc aataaactgc aagcctgcgg cggctctatt tccgccacta
    17581 tctactttat tcgccgcaaa gtggttagtt taagcgatca gaaactcaat attgccatga
    17641 cctttgtcgg ctcaatgagc ggcgcactgt tggtacagta cgttcaggct gatgtcttac
    17701 ggcagatttt gcccattctg gtgatttgta tcggcctcta tttcttgctg atgccaaagc
    17761 tgggtgaaga agatcgccag cgccggatgt acggtttacc cttcgcgtta attgccggtg
    17821 gctgcgtcgg tttttatgac ggattctttg gcccggctgc cggatcgttt tacgcgctgg
    17881 ccttcgttac gctgtgcgga ttcaacctcg ccaaagccac ggctcacgcc aaattactca
    17941 acgcaacgtc aaacattggc ggtctgctgc tgtttattct cggcggcaaa gtgatttggg
    18001 cgacgggctt tgtcatgctg gtggggcagt tcctcggcgc gcgcatgggt tcacggctgg
    18061 tgttgagcaa aggacaaaag ctgatccgcc cgatgattgt tatcgtctcg gcggtgatga
    18121 gtgccaaatt actttacgat agccacggac aggagatcct ccactggttg gggatgaact
    18181 aatgaacagt acacaccact atgagcagtt gattgaaatt tttaatagct gctttgccga
    18241 tgaatttaat acccgtctga ttaaaggcga cgacgaaccg atctatcttc ctgctgatgc
    18301 ggaagtaccg tataaccgaa ttgtctttgc ccacggcttt tatgccagcg caattcatga
    18361 gatttcgcac tggtgtattg ccggaaaagc gcgccgtgag ctggttgatt tcggctactg
    18421 gtattgcccg gatgggcgtg atgcccaaac gcaaagccag tttgaagatg ttgaagtgaa
    18481 gccgcaggca ctcgactggt tgttctgtgt ggcggcggga tatccgttta acgtcagttg
    18541 cgacaatctg gaaggcgact tcgaaccgga tcgtgttgtg tttcaacggc gggtacatgc
    18601 gcaggtgatg gattatctga caaatggcat tcccgagcgt ccggcacgct tcattaaagc
    18661 actacaaaat tattatcaca cgccggaact tacggcggaa cagttcccgt ggccggaagc
    18721 gctcaactga agccggtgcc acgttttaca atgaggaaag aagatgatcg cggagtttga
    18781 atcacgcatt ctggcattaa tcgacggcat ggttgaccat gccagtgatg atgagttgtt
    18841 tgccagtggg tatttgcgtg gccacctgac attagccatc gcagaactgg aaagtggtga
    18901 tgaccactcc gctcaggcgg tgcatacgac cgttagccag agtctggaaa aagccattgg
    18961 tgccggtgaa ttgtcgccgc gtgaccaggc gctggtgacc gatatgtggg aaaacctgtt
    19021 tcagcaggcg tcacagcagt aagttgcggc gtctggagca ccatgcctga tgcgacgctt
    19081 atcgcgtctt atcaggccta cacgtcacac gtaggtcgga taaggcgttc acgccgcatc
    19141 cggcaaacac tgcgagagca gattaccccg ccttaaccgc tttacccttc aacaatttcc
    19201 gcacccataa ccgattcggg tttaacgccg tcagcgtact ggcatccatc ggaatcggtt
    19261 cgtcgctcat ctgcgccgcc agaatctcgg cacacagcgg ggcagaacac aaaccgcgag
    19321 aacctaaagc cgcaaacata aagagatcgt caaaaaccgg cgcgcttacc gcctcatctt
    19381 tctgctccgc caacgacgca tattccacga gtgttgcctc ataatcggga acattgccta
    19441 ccattggcag atgatcgcgg gtggcacaac gcacaccgca gcgcgcctct ttatcactga
    19501 catcaacctc ttttgcccac tgtgcctgcg ggaaacaatc aatcaaccgc tggcgattct
    19561 gctgctgatc gtcctcactg tacgccgtat cttcgctgcc gcgatgataa ctggcaccaa
    19621 tacaatgatg ttgattcgcc ggattttgtg gcgtgagata accgtcatag cacagcacct
    19681 gcttcagctc tgccagttcc ggcgttgtcg gaatatggct gacctgcccg gcaaccgaat
    19741 acaccgggag agtcgacgtt tggctgaaac ggctgatttg atgcccgttc gccagtacca
    19801 ctacgctgtg tgttgcttgc tgatctcctg caaaattcaa caaccaacag tcatccttac
    19861 gggataaatt ctgtaactga tattgataat aaatctgcaa accctgctgt tgcgccagtt
    19921 ccagcacatt acgggtcagt tctgctgggc acagccaacc accttgcgga taagtaatgc
    19981 cgctgcaatt tgtcgcaacg cccgtaattt gttcaaccgc atttgcctca acggctacag
    20041 ccagttctgc gggtaaatcc attgacaaca tctgtgcgat tttatgctgg cttttctcat
    20101 cccagcctaa ctgcgtgacg ccgcaccagt catgatcaaa tttaacgggt aattggtcgt
    20161 aaaaccgacg agcaaaagta aacgcattag agaaaaagcg gtttagcgcc tcatcgtgtt
    20221 tgcttaataa cggatacagc gccccctggc gattgccgga agcacccagc gcgggggctt
    20281 catccgcgca ataaagcgct acctgccagc cgcgccgtaa taacgccagc gacaacaacg
    20341 cgctggcaat accaccgccg ataatcgcta cttcccgttt gctgctgccc gtacgattaa
    20401 accacggcgt ggagcagggg agtggtaatg tctgttccat caccccacaa agcatttccc
    20461 gtttgcgccc aaagccctta cgtttttgca tcgtgaatcc ggcttcctgc aaaccgcggc
    20521 ggacaaaacc ggcagacgta aatgtcgcca gcgtgccgcc tggacgcgcc aaccttgcca
    20581 tggcgttaaa cagattttgc gtccacatat ccgggttttt agctggcgca aagccgtcca
    20641 gaaaccaggc atctactttt tgatttagcg aatcgtccag ttggctggtc agttcgttaa
    20701 tatcgccaaa ccataaatcc agcgtcacgc ggccttcatt gagcaataaa cgatggcaac
    20761 cgggcaaggg cattggccac tgcgcctgaa gttgttctgc ccacggagcc agttccggcc
    20821 agtgttgatg cgctaaggct aaatccgcac gggtgagggg aaatttctca aaactaatga
    20881 aatgtaagcg ttgtaattgc gcttgcggat gcgcttcgcg aaactgatca aatgcctgcc
    20941 atagcgtcag gaagtttaat ccggtgccga agccgctctc tgctaccaca aacagaggat
    21001 gtggatgctc aggaaagcgt acctctaatt ggttgcctcc cagaaaaaca taacgcgtct
    21061 cttccagccc gttatcgttg gaaaaataga catcgtcaaa atctcgggaa acaggtgtac
    21121 cctcagcatt aaattcgagg ttggcaggtt gtatggagta gtgtttcacg taagttactc
    21181 gtcttacagg cggtggctcg atcttagcga tgtgtgtaag gctgcgcaaa tttctctatt
    21241 aaatggctga tcggacttgt tcggcgtaca agtgtacgct attgtgcatt cgaaacttac
    21301 tctatgtgcg acttacagag gtattgaatg aaacgtgcag tgattactgg cctgggcatt
    21361 gtttccagca tcggtaataa ccagcaggaa gtcctggcat ctctgcgtga aggacgttca
    21421 gggatcactt tctctcagga gctgaaggat tccggcatgc gtagccacgt ctggggcaac
    21481 gtaaaactgg ataccactgg cctcattgac cgcaaagttg tgcgctttat gagcgacgca
    21541 tccatttatg cattcctttc tatggagcag gcaatcgcag atgcgggcct ttctccggaa
    21601 gcttaccaga ataacccgcg cgttggcctg attgcaggtt ctggcggcgg ttccccgcgt
    21661 ttccaggtat tcggcgctga cgcaatgcgc ggcccgcgcg gcctgaaagc ggttggcccg
    21721 tatgtggtca ccaaagcgat ggcatccggc gtttctgcct gcctcgccac gccgtttaaa
    21781 attcatggcg ttaactactc catcagctcc gcgtgtgcga cttccgcaca ctgtatcggt
    21841 aacgcagtag agcagatcca actgggcaaa caggacatcg tgtttgctgg cggcggcgaa
    21901 gagctgtgct gggaaatggc ctgcgaattc gacgcgatgg gtgcgctgtc tactaaatac
    21961 aacgacaccc cggaaaaagc ctcccgtact tacgacgctc accgtgacgg tttcgttatc
    22021 gctggcggcg gcggtatggt agtggttgaa gagctggaac acgcgctggc gcgtggtgct
    22081 cacatctatg ctgaaatcgt tggctacggc gcaacctctg atggtgcaga catggttgct
    22141 ccgtctggcg aaggcgcagt acgttgtatg cagatggcga tgcatggcgt tgatacccca
    22201 atcgattacc tgaactctca cggtacttcg actccggttg gcgacgtgaa agagctggca
    22261 gctatccgtg aagtgttcgg cgataagagc ccggcgattt ctgcaaccaa agccatgacc
    22321 ggtcactcac tgggcgctgc gggtgtgcag gaagctatct actctctgct gatgctggaa
    22381 cacggcttta tcgccccgag catcaacatt gaagagctgg acgagcaggc tgcgggtctg
    22441 aacatcgtga ccgaaacgac cgatcgcgaa ctgaccactg ttatgtctaa cagcttcggc
    22501 ttcggcggca ccaacgccac gctggtaatg cgcaagctga aagattaatt cgcagtaggc
    22561 cggagtaggt cggataagac gcgccagcgt cgcatccgac gttacgcgcc aatgcggcct
    22621 ccggcactaa cgcaaaaggg aacctgatgg ttcccttttt cacatcattg acaatcgcca
    22681 ccagttccag gcaaacttcc cgctttgtcg atttccttct gaaaagacgt acgcgttaaa
    22741 tcctgccaac gcaccgtaac cctgaaacca gagagatgag acggggatac tcctcgcctt
    22801 gcgctgcatt ctggagtaat gcatgactgc tgtaagccaa accgaaacac gatcttctgc
    22861 caatttttcg ctcttccgca tcgcttttgc ggtttttctc acctacatga ccgtagggct
    22921 gccgttgccg gttatcccgc tgtttgttca tcatgaactg ggctatggca ataccatggt
    22981 cggcattgcc gtcgggattc agtttctggc tacggtgctg acgcgtggct atgccgggcg
    23041 actggccgat caatatggcg caaaacgttc ggcgcttcag gggatgttag cttgtggtct
    23101 ggctggcggc gcgttgctgc tggcggcgat tttgcctgtc tccgcaccgt tcaaatttgc
    23161 cctgttggtc atcgggcgtt tgattcttgg gtttggtgaa agccagttac tgacaggcgc
    23221 tctgacctgg gggttaggca tcgtagggcc aaaacactct ggcaaagtga tgtcatggaa
    23281 tggaatggcg atttacggtg ccctcgctgt tggtgctccg cttggcctgt tgattcatag
    23341 ccattacggt tttgccgcac tggcgatcac cacaatggta ttacccttac tggcgtgggc
    23401 ctgtaacggc acagtgcgca aagtaccggc cctggcggga gaacgtccat cgctgtggag
    23461 cgttgtcggg tttatctgga aaccagggtt agggctggca ctacaaggcg ttggttttgc
    23521 ggttatcggg actttcgttt cgctctactt tgccagcaaa ggatgggcga tggcgggctt
    23581 tactcttacc gagtttggcg gcgcatttgt cgtgatgcgc gtcatgtttg gctggatgcc
    23641 ggaccgtttt ggcggcgtga aagtggcgat tgtctctctg cttgtagaaa cggtgggctt
    23701 gttgctgctc tggcaagccc caggtgcgtg ggtcgcatta gcgggcgcgg cgttaaccgg
    23761 agccggatgt tcgcttatct ttcctgcgct gggcgtggag gtggttaaac gcgtcccctc
    23821 acaagttcgc ggcaccgcac tgggcggtta cgccgcgttt caggatatcg ccctcggcgt
    23881 ctccgggccg ctggcgggaa tgctggcgac cacgtttggt tactcttcgg tatttcttgc
    23941 cggggcgatc tctgcggtgc tgggaattat tgtcacgata ctgtcatttc gtcggggcta
    24001 acgaaccagc cagaccagca tcaggataat cgcgactaac agtatccaca gcgcgggacg
    24061 cgttgccata ttttgcaacg tgtccatcat cggtggaaaa ggattaagca gcggttgcat
    24121 atcacgcgga tcaatccctt taacccgccg ttgataaagc tgattaagaa tatcctcggc
    24181 ctgggcagaa gaaagcgatg attgcgcaga aagaccatat ttttgctgga tatacgccga
    24241 taacgctgcc agttcactgg catctaaagg ttgttttagc gccatctgta gtgattccag
    24301 cgtcggcgta ttttgctggc ttaacgtctg gcgcgcctgc aaccaggtca ccagatggtt
    24361 aaacagtttc gctggaatta actcgccatc gttcaccccg gaaagttcca gcatcgattg
    24421 ccagatctgt ttgctgggtt cccccgttgc cgccgcaagt ttggtcacca gctgttttag
    24481 cgcattgtgc tccgccggta ataaaggacg gtcggtcgcc tcgcgctgct gtggttgcgg
    24541 aataaccatc ttcccttcct gcaacagggt gagaatggtt tttaattgct ccggtgagag
    24601 ctgattcagc ggcgtctgac caaagttatg acggatataa tccgtgaccg cctgacgatt
    24661 attccccaga cgtaaatact cccctaattg cgctaaaagc tggcgggcag aatggctttt
    24721 ttgcgcggcc agcagacgtt gcgccagatt atgctcagcg gcagggaagt gacgcgaaag
    24781 cagcggtgaa tctcccgaca gaccaatatc gtgcctgata cccgcccaca gttctgctct
    24841 ttgttgttgc gtcagtgagg tcactttcgt cattaagctt tccagcgaag tacgttgctg
    24901 actggataaa ggctgattgc ccgcgccaga cggcaggtta tctccctgac ctggtggttg
    24961 cccaggagga gggccggaaa taggttgtat cattacgtat ccttatacct gaaatcttcg
    25021 caagtatgcc tggccgcgag attatggcac acttgtccgg ttaactctcg tctcatacag
    25081 gtaacacaaa cgtgaaaatc cttgttgatg aaaatatgcc ttatgcccgc gacttattta
    25141 gccgtttggg tgaggtgacc gcggttcccg ggcgtccaat ccccgtcgct caactggcag
    25201 acgcggatgc gctgatggtg cgttcggtca cgaaagtgaa tgaatctttg ctggcaggaa
    25261 aacccattaa atttgttggc actgccactg cggggaccga ccatgtcgat gaagcatggt
    25321 tgaagcaggc gggaattggt ttttccgctg cacctggctg taatgcgatt gcggtggtgg
    25381 aatatgtttt ctcctcacta ctgatgcttg ccgaacgcga tggattttca ctgcacgacc
    25441 gtacggtggg gatcgtgggc gttggtaacg ttggacgtcg attgcaggcg cgactggaag
    25501 cgttagggat taaaacattg ctttgcgatc cgcctcgcgc tgaccgtggg gatgagggtg
    25561 atttccgctc gctggatgaa ttagttcagc atgcggatat tctgactttc catacgccac
    25621 tcttcaaaga tgggccgtac aaaacgctgc atctggcgga tgaaaaactg atccgtagcc
    25681 tgaagccggg agcgattctg attaacgcct gccgtggcgc agtcgtcgat aatactgcgt
    25741 tgctgacctg cctgaatgaa ggccagaagt taagcgtagt gctggatgtc tgggaaggcg
    25801 aaccggaact taacgtggag ttgctgacaa aagtggatat cggcacgccg catatcgcag
    25861 gttataccct ggaaggtaaa gcacgcggta ctacgcaagt gtttgaagcc tatagcaagt
    25921 ttattgggca tgaacagcac gttgcgctgg atacattact gcccgcgcca gagtttggtc
    25981 gcattacgct gcatggcccg ctcgatcaac cgacgctgaa aaggctggtg catttggtgt
    26041 atgatgtgcg ccgcgatgac gcaccgctgc gtaaagtcgc cgggataccg ggtgagttcg
    26101 ataaactgcg caaaaactat cttgagcgcc gtgaatggtc atctctgtat gtaatttgtg
    26161 atgacgccag tgcggcatca ttgctgtgta aactgggttt taacgccgtt catcatccgg
    26221 cacgttaatc tcttcttcat gctctctgct gtaacattgg cagggagctt tgctatttct
    26281 ggagtaaacc accatgtctg aaggctggaa cattgccgtc ctgggcgcaa ctggcgctgt
    26341 gggcgaagcc ctgcttgaaa cgctggctga acgtcagttc ccggttgggg aaatttatgc
    26401 actggcacgt aacgaaagcg caggcgaaca actgcgcttt ggtggtaaaa caatcactgt
    26461 gcaggatgcc gctgaattcg actggacgca ggcgcagctg gcattttttg tcgcaggcaa
    26521 agaagctacc gctgcctggg ttgaagaagc gaccaactca ggttgcctgg tgatcgacag
    26581 cagcggactg tttgctctcg aacccgacgt accgctggtg gtgccggaag taaacccgtt
    26641 tgtactgacc gattaccgca accggaatgt catcgccgta ccagacagtc tgaccagcca
    26701 gctgctggcg gcactgaaac cgttaatcga tcagggcggt ttgtcgcgta tcagtgttac
    26761 cagcctgatt tcagcctccg cccagggcaa aaaagcggtc gatgcgttag cggggcagag
    26821 tgcgaaattg ctcaacggca ttccgattga cgaagaagat ttcttcggtc gtcaactggc
    26881 gttcaatatg ctgccgttgt tgccggatag cgaaggtagc gtgcgtgaag aacgtcgtat
    26941 cgttgacgaa gtacgcaaaa tcctgcagga cgaagggctg atgatttcgg caagcgtcgt
    27001 ccaggcaccg gtattctacg gtcatgccca gatggtcaac tttgaagcac tgcgtccgct
    27061 ggcggcagaa gaagcgcgtg atgcgtttgc tcagggcgaa gatattgtgc tctctgaaga
    27121 gaacgaattc ccgactcagg taggggatgc ttcgggtacg ccgcatcttt ctgttggctg
    27181 cgtgcgtaat gactacggta tgccggagca agtccagttc tggtcggtgg ccgataacgt
    27241 tcgctttggc ggcgcgctga tggcagtaaa aatcgccgag aaactggtgc aggagtatct
    27301 gtactaatgt ccgaccagca acaactgcca gtttataaaa ttgcgctggg cattgagtac
    27361 gacggcagta agtattacgg ctggcaacgg cagaatgaag tccgcagtgt gcaggagaag
    27421 ctggaaaagg cgctctccca ggtggcgaac gaacccatca ccgtcttctg cgccgggcgt
    27481 actgacgcag gggtacacgg taccgggcag gtggtgcatt tcgaaacaac cgcgcagcgc
    27541 aaagacgcgg cgtggacctt gggcgtaaat gcgaatttac ctggtgacat cgccgtgcgt
    27601 tgggttaaag ctgtacctga tgattttcat gcccgattta gcgccacggc tcgccgttat
    27661 cgctacatca tctacaatca tcggctgcgc ccggcggtat tgagtaaagg ggtaacccat
    27721 ttttacgaac cgctggacgc tgaacggatg catcgggctg cgcaatgctt gctgggcgag
    27781 aatgatttca cctcgttccg tgcggtgcag tgccagtccc gaaccccgtg gcgcaacgtt
    27841 atgcatatta atgtcacgcg tcacggtcct tatgtggtgg tagatatcaa agcgaatgcc
    27901 tttgtacatc atatggtcag gaatattgtc ggcagcctga tggaagtcgg tgcccacaac
    27961 cagccggaga gatggatagc agagttgctg gcggcaaagg acagaacgct ggcggcagca
    28021 acggcaaaag cggaagggct gtatctggtc gcggtggatt accctgaccg gtatgatctt
    28081 ccaaaaccgc caatgggccc gctatttctg gcggactaac gagaataatg ctcggaacat
    28141 ttcaggctgt agccaacgta gttacagcct gaaagatgac gagtacaagg cataggcaaa
    28201 tatggacctg atttatttcc tcatcgattt tatcctgcac attgacgtgc atctggcaga
    28261 gctggtcgcg gagtacggcg tctgggttta tgccattctg tttttgattc tgttctgcga
    28321 aacggggctg gtggtaacgc cattcctgcc tggtgattcg ctgctgtttg ttgctggtgc
    28381 attggcgtcg ctggaaacta acgatctcaa tgtccatatg atggtggtgc ttatgttgat
    28441 tgccgctatt gtcggtgatg cagtgaacta taccattgga cggttatttg gtgacaaact
    28501 gttcagtaac ccggattcga aaatctttcg tcgcagttat cttgataaga ctcatcagtt
    28561 ttacgagaga catggcggca aaacgattat tctcgcccgt tttgtaccga tcgtcagaac
    28621 gttcgcgccg tttgttgcgg gaatgggcca catgtcgtac cgtcatttcg ccgcttataa
    28681 cgtgatcggc gcactgttgt gggtactgat ttttacctac gcgggttatt tcttcggcac
    28741 gctccccttc attcagagca accttaagct gatgattgtt ggcattattt ttgtttcaat
    28801 attgcctggg gtcttcgaat ttattcgtca caaacgcgct gcggcacgcg ccgcaaaata
    28861 aaaagtaact ccgcggttcg accacttttt tatccaaagt ttcgggctgt tatgttttaa
    28921 tgtgcaacat tcatggtctg ttgggggcaa aaatggcatt atgcgtcccc aaagataaaa
    28981 ctggcatcga accaggttca gacagaaagg tccctaatga gctggattga acgaattaaa
    29041 agcaacatta ctcccacccg caaggcgagc attcctgaag gggtgtggac taagtgtgat
    29101 agctgcggtc aggttttata ccgcgctgag ctggaacgta atcttgaggt ctgtccgaag
    29161 tgtgaccatc acatgcgtat gacagcgcgt aatcgcctgc atagcctgtt agatgaagga
    29221 agccttgtgg agctgggtag cgagcttgag ccgaaagatg tgctgaagtt tcgtgactcc
    29281 aagaagtata aagaccgtct ggcttctgcg cagaaagaaa ccggcgaaaa agatgcgctg
    29341 gtggtgatga aaggcactct gtatggaatg ccggttgtcg ctgcggcatt cgagttcgcc
    29401 tttatgggcg gttcaatggg gtctgttgtg ggtgcacgtt tcgtgcgtgc cgttgagcag
    29461 gcgctggaag ataactgccc gctgatctgc ttctccgcct ctggtggcgc acgtatgcag
    29521 gaagcactga tgtcgctgat gcagatggcg aaaacctctg cggcactggc aaaaatgcag
    29581 gagcgcggct tgccgtacat ctccgtgctg accgacccga cgatgggcgg tgtttctgca
    29641 agtttcgcca tgctgggcga tctcaacatc gctgaaccga aagcgttaat tggctttgcc
    29701 ggtccgcgtg ttatcgaaca gaccgttcgc gaaaaactgc cgccaggatt ccagcgcagt
    29761 gaattcctga tcgagaaagg cgccatcgac atgatcgtcc atcgtccgga aatgcgcctg
    29821 aaactggcga gcattctggc gaagttgatg aatctgccag cgccgaatcc tgaagcgccg
    29881 cgtgaaggcg tagtggtacc cccggtaccg gatcaggaac ctgaggcctg ataactgata
    29941 agggcagggc cactggctct gcccttttgc tattctcacc gtaacgaatc agcggatacc
    30001 atgattatca aacgcactcc tcaagccgcg tcgcctctgg cttcgtggct ttcttatctg
    30061 gaaaacctgc acagtaaaac tatcgatctc ggccttgagc gcgtgagcca ggtcgcggcg
    30121 cgtcttggcg tcctgaaacc agcgccattt gtgtttaccg ttgcgggtac gaatggcaaa
    30181 ggcaccacct gccgtacgct ggagtcgatt ctgatggcgg cagggtacaa agtgggcgtc
    30241 tacagttcac cgcatctggt gcgttatacc gagcgcgtac gtgtgcaggg gcaggaattg
    30301 ccggaatcgg cccacaccgc ctcttttgcg gagattgaat cggcacgcgg tgatatttcc
    30361 ctgacctatt tcgagtacgg tacgctgtcg gcgttgtggc tgttcaaaca ggcacaactt
    30421 gacgtagtga ttctggaagt agggctgggc ggtcgtctgg acgcaaccaa tattgtcgat
    30481 gccgatgtgg ctgtagtaac cagcattgcg ctggatcata ccgactggct gggaccagat
    30541 cgcgaaagta ttggtcgcga gaaagcaggc atctttcgca gcgcaaaacc ggcaattgtc
    30601 ggtgagccgg aaatgccttc taccattgct gatgtggcgc aggaaaaagg tgcactgtta
    30661 caacgtcggg gcgttgagtg gaactattcc gtcaccgatc atgactgggc gtttagcgat
    30721 gctcacggca cgctggaaaa tctgccgttg ccgcttgtcc cgcaaccgaa tgccgcaaca
    30781 gcgctggcgg cactgcgtgc cagcgggctg gaagtcagtg aaaatgccat tcgcgacggg
    30841 attgccagcg caattttgcc gggacgtttc cagattgtga gcgagtcgcc acgcgttatt
    30901 tttgatgtcg cgcataatcc acatgcggcg gaatatctca ccgggcgtat gaaagcgcta
    30961 ccgaaaaacg ggcgcgtgct ggcggttatc ggtatgctac atgataaaga tattgccgga
    31021 actctggcct ggttgaaaag cgtggttgat gactggtatt gtgcgccact ggaagggccg
    31081 cgcggtgcca cggcagaaca actgcttgag catttgggta acggcaaatc atttgatagc
    31141 gttgcgcagg catgggatgc cgcaatggcg gacgctaaag cggaagacac cgtgctggtg
    31201 tgtggttctt tccacacggt cgcacatgtc atggaagtga ttgacgcgag gagaagcggt
    31261 ggcaagtaag tttcagaatc ggttagtggg cacgatcgtg ctggtggcgc tgggggtgat
    31321 tgtacttcca gggctgctgg acgggcagaa aaaacattat caggatgagt ttgcggctat
    31381 cccgctggtt ccaaaagcgg gcgatcgcga tgagcctgat atgatgccag ccgccaccca
    31441 ggcgttgccg acgcagccgc cggaaggcgc agcggaagag gtgcgggcag gtgatgccgc
    31501 agcaccgtcg ctcgatccgg ccactattgc agccaataac accgagtttg aaccggaacc
    31561 tgcaccggtc gccccaccga agccgaaacc ggtggagccg cctaaaccga aggttgaagc
    31621 accacctgcg ccgaagccag aaccgaagcc ggtcgtggaa gaaaaagcag cgccaacggg
    31681 taaagcctat gttgtgcaac tgggtgcgct gaaaaatgcc gataaagtga atgagattgt
    31741 cggtaagctg cgcggtgccg gttatcgggt ttatacgtcg ccatccacgc cagtgcaggg
    31801 taaaattacc cgtattctgg ttgggccgga tgcctcgaaa gataagctga aaggttcgct
    31861 gggtgagttg aagcaacttt ctggcttaag tggcgtggta atgggctata cgccgaatta
    31921 atacagtctt gcctgatgcg acgctggcgc gtcttatcag gcctgggcag tgttagaacc
    31981 gtaggtcgga taaggcgttt acgccgcatc cgacacgcat tgcacgatgc cgcaaaggca
    32041 taaaaagtcg atggcgttga atattttttc agcgccattt ttattgatgc gtgggaagga
    32101 aatccctacg caaacgtttt ctttttctgt tagaatgcgc cccgaacagg atgacagggc
    32161 gtaaaatcgt gggacacata tggtctggat tgattacgcc ataatcgcgg tgattgcttt
    32221 ttcctctctg gttagcctga tccgcggctt tgttcgtgaa gcgttatcgc tggtgacatg
    32281 gggttgtgct ttctttgttg ccagtcatta ctacacttac ctgtcagtct ggtttacggg
    32341 ctttgaagac gaactggttc gaaatggaat tgccatcgcg gtactgttta tcgctaccct
    32401 gattgttggt gctatcgtga acttcgtgat aggccagttg gtggagaaaa cggggttgtc
    32461 aggcaccgat cgggtgctgg gcgtctgttt cggtgcgttg cgcggtgtgt tgattgttgc
    32521 cgccattctc ttctttctcg actcctttac cggggtgtcg aaaagcgaag actggagcaa
    32581 atcacagctg atcccgcagt tcagttttat catcagatgg ttttttgatt atctgcaaag
    32641 ctcgtcaagt ttcttgccca gagcgtaagt gctctgagat gtggcttaac gaggaaaaag
    32701 acgtatgtgc ggtattgtcg gtatcgccgg tgttatgccg gttaaccagt cgatttatga
    32761 tgccttaacg gtgcttcagc atcgcggtca ggatgccgcc ggcatcatca ccatagatgc
    32821 caataactgc ttccgtttgc gtaaagcgaa cgggctggtg agcgatgtat ttgaagctcg
    32881 ccatatgcag cgtttgcagg gcaatatggg cattggtcat gtgcgttacc ctacggctgg
    32941 cagctccagc gcctctgaag cgcagccgtt ttacgttaac tccccgtatg gcattacgct
    33001 tgcccacaac ggcaatctga ccaacgctca cgagttgcgt aaaaaactgt ttgaagaaaa
    33061 acgccgccac atcaacacca cttccgactc ggaaattctg cttaatatct tcgctagcga
    33121 gctggacaac ttccgccact acccgctgga agccgacaat attttcgctg ccattgctgc
    33181 cacaaaccgc ttaatccgcg gcgcatatgc ctgtgtggcg atgattatcg gccacggtat
    33241 ggttgctttc cgcgatccaa acgggattcg tccgctggta ctgggaaaac gtgatattga
    33301 cgagaaccgt acagaatata tggtcgcttc cgaaagcgta gcgctcgata cgctgggctt
    33361 tgatttcctg cgtgacgtcg cgccgggcga agcgatttac atcactgaag aagggcagtt
    33421 gtttacccgt caatgtgctg acaatccggt cagcaatccg tgcctgtttg agtatgtata
    33481 ctttgctcgc ccggactcgt tcatcgacaa aatttccgtt tacagcgcgc gtgtgaatat
    33541 gggcacgaaa ctgggcgaga aaattgcccg cgaatgggaa gatctggata tcgacgtggt
    33601 gatcccgatc ccagaaacct cgtgtgatat cgcgctggaa attgctcgta ttctgggcaa
    33661 accgtaccgc cagggcttcg ttaaaaaccg ctatgttggc cgcaccttta tcatgccggg
    33721 ccagcagctg cgtcgtaagt ccgtgcgccg taaactgaat gccaaccgcg ccgagttccg
    33781 cgataaaaac gtcctgctgg tcgacgactc catcgtccgt ggcaccactt ctgagcagat
    33841 tatcgagatg gcacgcgaag ccggagcgaa gaaagtgtac ctcgcttctg cggcaccgga
    33901 aattcgcttc ccgaacgttt atggtattga tatgccgagc gccacggaac tgatcgctca
    33961 cggtcgcgaa gttgatgaaa ttcgccagat catcggtgct gacgggttga ttttccagga
    34021 tctgaacgat ctgatcgacg ccgttcgcgc tgaaaatccg gatatccagc agtttgaatg
    34081 ctcggtgttc aacggcgtct acgtcaccaa agatgttgat cagggctacc tcgatttcct
    34141 cgatacgtta cgtaatgatg acgccaaagc agtgcaacgt cagaacgaag tggaaaatct
    34201 cgaaatgcat aacgaaggat gatgccttcg ctgagggtgc cggtctggca ccctgacttg
    34261 caactcccgc cgaaatactg caaagtctgc ctgcaagtct gacagggcaa ctatttatga
    34321 aacgactcat tgtaggcatc agcggtgcca gcggcgcgat ttatggcgtg cgcttattac
    34381 aggttctgcg cgatgtcaca gatatcgaaa cgcatctggt gatgagccag gcggcgcgcc
    34441 agaccttatc cctcgaaacg gatttttccc tgcgcgaagt gcaggcatta gctgatgtca
    34501 cgcacgatgc gcgcgatatt tccgccagca tctcttccgg ttctttccag acgctgggga
    34561 tggtgatttt accctgttca atcaaaaccc tttccggcat tgtccatagc tatactgatg
    34621 gcttactgac ccgtgcggca gatgtggtgc tgaaagagcg tcgcccgttg gtgctctgcg
    34681 tgcgtgaaac accattgcac ttaggccatc tgcgtttaat gactcaggcg gcagaaatcg
    34741 gtgcggtgat tatgcctccc gttccggcgt tttatcatcg cccgcaatcc cttgatgatg
    34801 tgataaatca gacggttaat cgtgttcttg accagtttgc aataaccctt cctgaagatc
    34861 tctttgcccg ctggcagggc gcataactcc gctgttgccc tgtttcaggg caattttgca
    34921 accgcgatca aatcctcgac attttgtttc tgccattcaa tcgaaacgct gcgattcaac
    34981 cgctatacct gctatcttca acttcaggac aataatgcaa cgtcttatta acatatttaa
    35041 cgttgaatgt tactgttgtc gtcaagatgg cataagacct gcatgaaaga gcctgcaaac
    35101 acacaacaca atacacaaca taaaaaagcc attttcactt gagggttatg tatgaagaag
    35161 tcgattctcg ctctgtcttt gttagtcggt ctctccgcag cggcttccag ctatgcggcg
    35221 ctaccggaga cggtacgtat cggaaccgat accacctatg cgccgttctc gtcgaaagat
    35281 gctaaaggtg attttgttgg ctttgatatc gatctcggta acgagatgtg caaacggatg
    35341 caggtgaaat gtacctgggt tgccagtgac tttgacgcgc tgatcccctc actgaaagcg
    35401 aaaaaaatcg acgccattat ttcgtcgctt tccattaccg ataaacgtca gcaggagatt
    35461 gccttctccg acaagctgta cgccgcagat tctcgtttga ttgctgccaa aggttcaccg
    35521 attcagccaa cgctggattc actgaaaggt aaacatgttg gtgtgctgca gggatcaacc
    35581 caggaagctt acgctaacga gacctggcgt agcaaaggcg tggatgtggt ggcctatgcc
    35641 aaccaggatt tggtctattc cgatctggct gcaggacgtc tggatgctgc gttacaagat
    35701 gaagttgctg ccagcgaagg attcctcaag caacctgctg gtaaagcttt cgcctttgct
    35761 ggctcatcag taaaagacaa aaaatacttc ggtgatggca ccggtgtagg gctacgtaaa
    35821 gatgatgctg aactgacggc tgccttcaat aaggcgcttg gcgagctgcg tcaggacggc
    35881 acctacgaca agatggcgaa aaagtatttc gactttaatg tctacggtga ctgatacgtc
    35941 gctgggaagc tgtacctgat ggaatgatca tcatggtgca cgccaggttt gttgcactat
    36001 cgtggtgcat tgaaatgcat acttaagcat ttttaatgaa aaataatacg tctaacgggg
    36061 cggaatattt tgccttgatg gtcaatttta tggcacgata agtgtaacaa acctgtaaat
    36121 attccctata aaaagactgt cagttgagga cattatgaaa aaactggtgc tatcgctctc
    36181 tctggttctg gccttctcca gcgcaactgc ggcatttgct gcgattccgc aaaacatccg
    36241 catcggtacc gacccgacct atgcgccatt tgaatcaaaa aattcacaag gcgaactggt
    36301 tggcttcgat atcgatctgg caaaggaatt atgcaaacgc atcaatacgc aatgtacgtt
    36361 tgtcgaaaat ccgctggatg cgttaatccc gtccttaaaa gcgaagaaga ttgacgccat
    36421 catgtcatcg ctttccatta cggaaaaacg tcagcaagaa atagccttca ccgacaaact
    36481 gtacgctgcc gattctcgtt tggtggtggc gaaaaattct gacattcagc cgacagtcga
    36541 gtcgctgaaa ggcaaacggg taggcgtatt gcagggcacc acccaggaga cgttcggtaa
    36601 tgaacattgg gcaccaaaag gcattgaaat cgtctcgtat caggggcagg acaacattta
    36661 ttctgacctg actgccggac gtattgatgc cgcgttccag gatgaggtcg ctgccagcga
    36721 aggtttcctc aaacaacctg tcggtaaaga ttacaaattc ggtggcccgt ctgttaaaga
    36781 tgaaaaactg tttggcgtag ggaccggcat gggcctgcgt aaagaagata acgaactgcg
    36841 cgaagcactg aacaaagcct ttgccgaaat gcgcgctgac ggtacttacg agaaattagc
    36901 gaaaaagtac ttcgattttg atgtttatgg tggctaatcg ccatcggtga aagaaagccc
    36961 gctcccttcg ggtaaccgga gggagacaag aggcggtaat tcaccacaca cgacaggaca
    37021 ggcagcatgt tgtatgggtt ttcaggtgtt attttacagg gtgcgctcgt cacgctggag
    37081 ctggctatca gctctgtagt gctcgctgta atcatcggtt taattggcgc tggcggtaag
    37141 ctctcgcaaa atcggctttc ggggctgatt ttcgaagggt acaccacgct gattcgtggc
    37201 gtgccggact tagtgttgat gctgctgatt ttctacggtt tgcagattgc gctaaacacg
    37261 gtgacggagg cgatgggcgt cgggcagatt gatatcgatc cgatggtcgc tggtattatc
    37321 actctcggtt ttatctacgg tgcttatttt accgaaacgt tccgtggtgc ttttatggca
    37381 gtgccgaaag gacatataga ggcggcgacg gcgttcggtt ttactcgtgg gcaagtgttt
    37441 cggcggatca tgtttccggc gatgatgcgt tacgccctgc caggcattgg caacaactgg
    37501 caggtgatcc tcaaatctac cgcactggtt tcgttactcg gcctggaaga tgtggtcaaa
    37561 gccacccaac tggcaggcaa aagtacctgg gaaccgttct atttcgccat cgtctgtggc
    37621 gtgatttacc tggttttcac cactgtttcc aatggtgtgc tgctgttcct tgagcgccgc
    37681 tactccgtgg gtgtgaagag ggctgacctg tgatcgaaat cttacatgaa tactggaaac
    37741 cgctgctgtg gaccgacggt tatcgcttta ctggtgtggc gatcactctg tggctgctta
    37801 ttttgtcggt agtgataggc ggagtcctgg cgctgtttct ggcgattggt cgtgtctcca
    37861 gtaataaata catccagttt ccaatctggt tatttaccta tatttttcgc ggtacgccgc
    37921 tgtatgttca gttgctggtg ttctattccg gcatgtacac gcttgagatt gttaagggaa
    37981 ccgaattcct taacgctttc ttccgcagtg gcctgaactg taccgtgctg gcgctgacgc
    38041 ttaacacctg cgcttacact accgagattt ttgctggggc aatccgttcg gttccgcatg
    38101 gggaaattga agccgccaga gcctatggct tctcgacttt taaaatgtat cgttgcatta
    38161 ttttgccttc tgcgctgcgt attgcgttac cggcatacag caacgaagtg atcctgatgc
    38221 tgcactctac tgcgctggcg tttacggcca ctgtgccgga tctgctgaaa atagcccgcg
    38281 atattaacgc cgccacgtat caacctttta ccgccttcgg cattgccgcg gtgctctatt
    38341 taatcatctc ttatgtcctg atcagcctct ttcgcagagc ggaaaaacgc tggttgcagc
    38401 atgtgaaacc ttcttcaacg cactgagaat acgatgtccg agaataaatt aaacgttatc
    38461 gatttgcaca aacgctacgg cgaacatgaa gtgctgaaag gggtctcact gcaagcgaat
    38521 gccggagatg taataagcat catcggatcg tcgggatcgg ggaaaagtac ctttctgcgc
    38581 tgcattaact tcctcgaaaa accgagtgaa gggtcgatcg tggtcaatgg ccagacgatc
    38641 aatctggtgc gcgacaaaga cggtcaactc aaagtcgccg ataaaaatca actgcgctta
    38701 ctgcgcacac gcctgacgat ggtattccag cacttcaatc tctggagcca tatgacggtg
    38761 ctggaaaacg tcatggaagc gccgattcag gtgttgggcc tgagcaagca ggaagcgcgc
    38821 gagcgggcgg tgaagtatct ggcgaaagtc gggatagacg aacgtgcgca ggggaaatat
    38881 ccggtgcatc tttccggcgg tcagcaacag cgtgtttcta tcgcgcgggc gctggcgatg
    38941 gaaccggaag ttttactgtt tgatgaacct acctcggcgc tcgatcctga actggtaggc
    39001 gaagtgttac gtattatgca gcaactggca gaagagggga aaaccatggt ggtagtgact
    39061 cacgaaatgg gctttgctcg ccatgtttct actcacgtca ttttcctcca tcaggggaaa
    39121 atagaagaag agggcgcgcc ggagcagtta tttggcaacc cgcaaagccc tcgtctgcaa
    39181 cagttcctta agggatcgct gaaataattt cctctccggt ggctaacgct gccggagtat
    39241 tttcatgggg aatttgcgag tcagcttccg ctgaaatgta aaagttgcag tccgctaaaa
    39301 atacttctga attcgctgtc caaagataaa aactgcctgc tgcattgggt gttaatcgtg
    39361 gttattgtcg cgtcatctta aatttgaggg gtgacggaac gccatgacaa tatcgacaac
    39421 ttccacgccg catgatgcgg tatttaaatc tttttta
//
LOCUS       DAJVRF010000020        39407 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJVRF010000020 DAJVRF010000000
VERSION     DAJVRF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.020"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..266
                     /locus_tag="OQN13_000968"
     CDS             <1..266
                     /locus_tag="OQN13_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCS1614394.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="OQN13_000969"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="OQN13_000969"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCS1614395.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="OQN13_000970"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="OQN13_000970"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCS1614396.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="OQN13_000971"
     CDS             complement(1570..3270)
                     /locus_tag="OQN13_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCS1614397.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="OQN13_000972"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="OQN13_000972"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCS1614398.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4340..4798
                     /gene="decR"
                     /locus_tag="OQN13_000973"
     CDS             4340..4798
                     /gene="decR"
                     /locus_tag="OQN13_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCS1614399.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            4828..6600
                     /locus_tag="OQN13_000974"
     CDS             4828..6600
                     /locus_tag="OQN13_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS1614400.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="OQN13_000975"
     CDS             6593..8374
                     /locus_tag="OQN13_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS1614401.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="OQN13_000976"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="OQN13_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCS1614402.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="OQN13_000977"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="OQN13_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCS1614403.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="OQN13_000978"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="OQN13_000978"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCS1614404.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="OQN13_000979"
     CDS             11336..11908
                     /locus_tag="OQN13_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCS1614405.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="OQN13_000980"
     CDS             complement(11939..12250)
                     /locus_tag="OQN13_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCS1614406.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="OQN13_000981"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OQN13_000981"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="OQN13_000982"
     CDS             12629..12982
                     /locus_tag="OQN13_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCS1614407.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="OQN13_000983"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="OQN13_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..15206)
                     /locus_tag="OQN13_000984"
     CDS             complement(14736..15206)
                     /locus_tag="OQN13_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCS1614408.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15322..15873)
                     /gene="maa"
                     /locus_tag="OQN13_000985"
     CDS             complement(15322..15873)
                     /gene="maa"
                     /locus_tag="OQN13_000985"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCS1614409.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16044..16262)
                     /gene="hha"
                     /locus_tag="OQN13_000986"
     CDS             complement(16044..16262)
                     /gene="hha"
                     /locus_tag="OQN13_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCS1614410.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="OQN13_000987"
     CDS             complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="OQN13_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCS1614411.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIETFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(17208..20357)
                     /locus_tag="OQN13_000988"
     CDS             complement(17208..20357)
                     /locus_tag="OQN13_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCS1614412.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="OQN13_000989"
     CDS             complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="OQN13_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCS1614413.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            21715..22362
                     /gene="acrR"
                     /locus_tag="OQN13_000990"
     CDS             21715..22362
                     /gene="acrR"
                     /locus_tag="OQN13_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCS1614414.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            22490..25852
                     /gene="mscK"
                     /locus_tag="OQN13_000991"
     CDS             22490..25852
                     /gene="mscK"
                     /locus_tag="OQN13_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCS1614415.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="OQN13_000992"
     CDS             complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="OQN13_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCS1614416.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDKAEK"
     gene            complement(26239..26766)
                     /gene="priC"
                     /locus_tag="OQN13_000993"
     CDS             complement(26239..26766)
                     /gene="priC"
                     /locus_tag="OQN13_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCS1614417.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            26836..27213
                     /locus_tag="OQN13_000994"
     CDS             26836..27213
                     /locus_tag="OQN13_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCS1614418.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            27366..27917
                     /gene="apt"
                     /locus_tag="OQN13_000995"
     CDS             27366..27917
                     /gene="apt"
                     /locus_tag="OQN13_000995"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCS1614419.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            28046..29971
                     /gene="dnaX"
                     /locus_tag="OQN13_000996"
     CDS             28046..29971
                     /gene="dnaX"
                     /locus_tag="OQN13_000996"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCS1614420.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    29304..29368
                     /gene="dnaX"
                     /locus_tag="OQN13_000996"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            30024..30353
                     /locus_tag="OQN13_000997"
     CDS             30024..30353
                     /locus_tag="OQN13_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCS1614421.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            30353..30958
                     /gene="recR"
                     /locus_tag="OQN13_000998"
     CDS             30353..30958
                     /gene="recR"
                     /locus_tag="OQN13_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCS1614422.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            31068..32942
                     /gene="htpG"
                     /locus_tag="OQN13_000999"
     CDS             31068..32942
                     /gene="htpG"
                     /locus_tag="OQN13_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCS1614423.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            33123..33767
                     /gene="adk"
                     /locus_tag="OQN13_001000"
     CDS             33123..33767
                     /gene="adk"
                     /locus_tag="OQN13_001000"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCS1614424.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            33899..34861
                     /gene="hemH"
                     /locus_tag="OQN13_001001"
     CDS             33899..34861
                     /gene="hemH"
                     /locus_tag="OQN13_001001"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCS1614425.1"
                     /translation="MRQTKIGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(34858..35817)
                     /gene="aes"
                     /locus_tag="OQN13_001002"
     CDS             complement(34858..35817)
                     /gene="aes"
                     /locus_tag="OQN13_001002"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCS1614426.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            35969..37273
                     /gene="gsk"
                     /locus_tag="OQN13_001003"
     CDS             35969..37273
                     /gene="gsk"
                     /locus_tag="OQN13_001003"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCS1614427.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(37406..39082)
                     /locus_tag="OQN13_001004"
     CDS             complement(37406..39082)
                     /locus_tag="OQN13_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCS1614428.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
BASE COUNT         9526 a        10047 c        10418 g         9416 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 tcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgcaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    15001 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    15061 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    15121 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    15181 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    15241 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    15301 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    15361 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    15421 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    15481 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    15541 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    15601 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    15661 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    15721 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    15781 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    15841 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    15901 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    15961 gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta
    16021 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    16081 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    16141 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    16201 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
    16261 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    16321 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    16381 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
    16441 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtctcaat
    16501 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
    16561 atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt
    16621 aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
    16681 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
    16741 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
    16801 attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag
    16861 caggtaaaag cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat
    16921 tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
    16981 aattaccgca aataattaag aatagcctct aaatgattat ggattattaa atctattagc
    17041 gctacttaat ataaattaac taacaatcag tattttttat gaattttatc cgtggttaat
    17101 actggttttc gtatgagatc ctgagttggt ggttcaatta ctccttaatg ttcgtaggtt
    17161 atgcataaaa aaggccgctt gcgcggcctt agtgattaca cgttgtatca atgatgatcg
    17221 acagtatggc tgtgctcgat atcttcattc ttgcggctaa agcggcggcg aaccaccaca
    17281 aagaataccg gaacgaagaa gattgccagt accgttgcgg tcaccatccc gcccattaca
    17341 ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag tactgataac cagcggcata
    17401 acgccgagga taaacgccag cgaggtcatc aggatcggac gtaaacgcat ccgcactgca
    17461 tcaagcgtcg cttcaatcag acctttacct tctttatcca tcaagtcttt ggcgaattcg
    17521 acgataagta tcgcgttctt cgccgacaac ccaatggttg tgagcaggcc tacctggaag
    17581 taaacgtcat tggtcaggcc acggaaggtg gcagccagca acgcaccgat aacccccagc
    17641 ggaacgacca gcataacgga gaacggaatc gaccagctct cgtacagcgc cgccagacac
    17701 aggaatacga caatcaacga aatcgcgtac agtgaaggtg cctggttgcc ggagagacgt
    17761 tcctgatagg acatccccgt ccagtcatag ccaacaccgg taggcagttt gctcgccagt
    17821 tgttccatca gctccattgc ttcaccggta cttttacccg gtgccgcctg gcctaagatt
    17881 tccatggatg gcaggccgtt gtaacgttcc agacgcggcg aaccgtactc ccaacgagaa
    17941 gaggagaacg ccgagaatgg caccatctga ccatcagcag cacgaacata ccagtcgccg
    18001 atatcatccg gcagcatacg gtatttcgct tctgacatga cgtaaacttt cttcacacga
    18061 ccgcggtcga taaagtcgtt cacatagcta ccgccccatg cagcgcccag agaggtgtta
    18121 atgtcgttga tagaaacacc cagcgcctgc gctttttcct ggtcgatatc aatcttaaac
    18181 tgcggggtat cttccagacc gtttggacgt acgctggtca gcatatcagg gtgcttcgct
    18241 gcttctgcaa gcagctggtt acgcgcctga gtcagttttt cgtgaccaag gccagcctgg
    18301 tcaatcagct caaagtcaaa gccggttgca gtacccagtt ccacgattgc gggcaggtta
    18361 aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcgc gtgttgcacg catggtaatc
    18421 gcttcaactt tgttttcttc gcccggacga tcggcccagt ccttcaagga aacgaacgca
    18481 ataccggtat tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa caccgactca
    18541 acgttgttct tttctttggt cagatagtaa tgcgttacct cattgagcac tttctgcgta
    18601 cgttcctgcg ttgcacctgc tggcagctga accatggtca taaatacgcc ctggtcctca
    18661 tctggcaaga aggagcttgg cagacgcacg aacagatagg ccatgccgac cacgatgatc
    18721 agatacagca ccaggtaacg ccccgtactg cgcagaatac cgcctacgct gtcggtgtag
    18781 tggtgcgtgc tcttctcgaa catgcggtta aaccagccga agaagccttt tttaccttcc
    18841 ccgtgatcgc ctttggcaat cggtttcagc atggtggcac aaagagccgg agtcaggatc
    18901 aacgctacca gtaccgacag cgccattgct gaaacaatgg taatagagaa ctgacgatag
    18961 atagcaccag tagaaccgcc aaagaaggcc atcggtacga ataccgccga cagtaccatc
    19021 gcgataccga ccagagcgcc ctgaatctgc cccatcgact tacgggtagc ttcttttggc
    19081 ggcaaacctt cttccgccat aacacgctcc acgttttcta ccacaacgat agcgtcatcc
    19141 accaacaagc cgatggcgag caccatcccg aacattgtta gcgtgtttat cgagaagcca
    19201 aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg gtacggcaat ggtcggaatc
    19261 aacgtcgcgc ggaagttctg caggaacaga tacataacca ggaacacgag gatgatcgct
    19321 tcgaccagcg ttttaaccac ttcgtgaata gagattttca cgaacggcgt ggtgtcgtac
    19381 gggtaaacaa ttttcagacc cgacgggaag aacggttcca tcttcgccag ttcagcacgg
    19441 attgccgcag cggtatccag cgcgtttgca ccggtcgcca gcttgatccc cagaccggaa
    19501 gccggttggc cgttaaactc tgcgatgatg tcgtagttct caccacccag ctcaatcttc
    19561 gccacatcac gcagcagtac gcgggaacca tcctgattca ctttcagcag gattttgccg
    19621 aactcttcag tagaggtcag acgcgtctga gcaataatag aggcgttaag ctgttggcct
    19681 ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct gggcgttctg cgctttgatg
    19741 gcggtaatga catcaaccgg cgttagctgg aatttgttca gctcattcgg gttcatccag
    19801 atacgcatcg cgtactgtga accgaacaac tgaacatcac ccacgcccga cgtacggctg
    19861 atggcatctt tcatattcgc cgctacgtag tcagagatat cctcctgcgt catggtgcca
    19921 tcggtgttga taacgccgac aaccatcagg aagctgctgg atgatttctc aacgctcacc
    19981 ccttgctgct gaacttcttg cggcagcaac ggcatcgcca gctgcagttt gttctgcacc
    20041 tgaacctgcg cgatatccgc atcagtacca gactcaaagg tcagggtgat ctgcacggta
    20101 cccgtggagt cactgttaga ggacatgtac atcaggttat cgataccgtt catattctgt
    20161 tcgataacct gtgtcaccgt gtcctgcact gttttcgcat cagcgccggg gtaggaggcg
    20221 gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag tttgaggatc
    20281 gccagccccc ctgccaacat gatgataatg gcgatcaccc acgcaaaaat cgggcgatcg
    20341 ataaagaaat taggcatgtc ttaacggctc ctgtttaagt taagacttgg actgttcagg
    20401 ctgagcaccg cttgcggctt gctggttatt atcagcggta acttcttgtg cttttacctg
    20461 gacaccagga cgcactttct gcagcccact tattactacg cgatcgcctg ctttcagacc
    20521 ttctgtcacc agccacttat cgccaatagc ctggcttgca acgatcggac gggtttccac
    20581 tttgtcatcc gcgccaacta ccagtacggt ggcatcacca cgcggcgtac gggttacgcc
    20641 ctgttgcggg actaaaatag cgtttggatt aagcccttct tccagacgtg cgcgcacgaa
    20701 catacccggc agcagagtgt gatccgggtt cgggaagata gcgcgtaggg tgatagaccc
    20761 agtggtctga tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg ggaacttaat
    20821 gccgtcactg gtgatcagcg acactttggc tttgccgttc tcttgtttca gcgtgccatt
    20881 cgccagttcc tgtttcaggc gcaggaagtc gttgctggac tgggtcacat caaggtagat
    20941 cggatcaagt tgctgcacgg ttgccagcgc agtcgcctga ccgttctgta ccaatgcgcc
    21001 ttccgtcacg ttcgatttac caatgcgacc gctaatcgga gaggtgactt tggtgtaagc
    21061 cagattgatc cgcgcagttt caacggcagc tttcgccgca gttaccgcag cattcgcctg
    21121 ttgcgcatca gccagagcct gatcgtactc ttgcttactg atgtactgag taccgagcag
    21181 tttctgataa cgattcaccg tcaattgcgc gatattggct gcagcctggg ctttcgccag
    21241 atcacctttc gcactgtcgt atgtcgcctg ataggtcgca ggatcaatct gatagagaga
    21301 gacaccagct tcgatgtcgc taccttcttt gaaattacgc ttcaggataa tcccgctaac
    21361 ttgaggacga acttctgcga tccggtaggc actggtgcga cccggaagct cggttgtgat
    21421 ctgcagaggt tcagttttga ctgttactac gccaacggcg ggcatctgct ggccaccttg
    21481 ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg cctgagagca tcagaacgac
    21541 cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt ccgatttcaa
    21601 attggtcaat ggtcaaaagt taataaaccc attgctgcgt ttatattatc gtcgtgctat
    21661 ggtacataca ttcacaaatg tatgtaaatc taacgcctgt aaattcacga acatatggca
    21721 cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt ggctctacgt
    21781 cttttctcac agcagggggt atcatccacc tcgctgggcg agattgcaaa agcagctggc
    21841 gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt cggatttgtt cagtgagatc
    21901 tgggaactgt cagaatccaa tattggtgaa ctagagcttg agtatcaggc aaaattccct
    21961 ggcgatccac tctcagtatt aagagagata ttaattcatg ttcttgaatc cacggtgaca
    22021 gaagaacgac gtcgattatt gatggagatt atattccaca aatgcgaatt tgtcggagaa
    22081 atggctgttg tgcaacaggc acaacgtaat ctctgtctgg aaagttatga ccgtatagaa
    22141 caaacgttaa aacattgtat tgaagcgaaa atgttgcctg cggatttaat gacgcgtcgg
    22201 gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct ctttgccccg
    22261 caatcttttg atcttaaaaa agaagcccgc gattacgtcg ccatcttact ggagatgtat
    22321 ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat aaccctgaat ctgactccag
    22381 gatttttcct ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg atattcttgc
    22441 ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct tcattcatca tgactatgtt
    22501 ccagtattac aaacgatcac ggcattttgt tttttcagca tttattgctt ttgtttttgt
    22561 cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg aatggtgatc tgccgacaaa
    22621 agcggacctg caggcgcaac ttgactcact aaataaacaa aaagatcttt ctgctcagga
    22681 caaactggtg cagcaggatc tgacagatac attagccacc ctcgataaaa tcgatcgcat
    22741 aaaagaagag acagttcagc tacggcaaaa agtcgctgaa gcgccggaaa aaatgcgcca
    22801 ggcgaccgcg gcgttaacag cacttagcga tgtcgataac gacgaagaaa cgcgcaaaat
    22861 tctgagtacg ctttcgttgc gccagctgga aactcgcgtt gcccaggcgc tggacgattt
    22921 gcaaaacgca caaaacgatc tggcgtctta taacagccag ctggtttcgt tacagacgca
    22981 gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag cagctgcaac aaattcgcag
    23041 tcgtctggat gggactgatg tcggcgagac tgccttacgt cccagccaga aagtgttaat
    23101 gcaggctcag caggcgttgc tgaatgcgga gattgaccag cagcgtaaaa gcctggaagg
    23161 gaacaccgtc ttgcaggata ccttgcaaaa gcaacgtgat tacgtgacgg cgaacagcgc
    23221 ccgtctggag caccagttgc agctgttgca agaagcggta aacagcaagc gcctgacttt
    23281 aactgaaaaa acggcgcagg aagccgtctc cccggatgaa gccgcgcgta ttcaggctaa
    23341 tccgctggtg aaacaggaac tggaaattaa ccagcagtta agtcagcgtc tgattaccgc
    23401 gactgaaaac ggtaatcagt tgatgcagca aaacattaaa gtcaaaaact ggctggagcg
    23461 ggcgctgcaa tcggaacgca atattaaaga gcagattgcc gtcctgaagg gcagcctgct
    23521 gttgtctcgt atcctttacc agcaacaaca aacgctgccc tcggcggatg aactggaaaa
    23581 catgaccaac cgcatcgcgg atttgcgtct cgaacagttt gaagttaacc agcagcgtga
    23641 tgcactcttc cagagcgatg cgttcgtcaa caaactggaa gaaggtcaca ccaacgaagt
    23701 caacagcgaa gttcacgatg cgttattgca agtggttgat atgcgtcgcg aattgctgga
    23761 tcaactcaac aaacagttgg gtaaccagct gatgatggcc attaacctgc aaatcaacca
    23821 gcagcagtta atgagcgtat cgaaaaacct gaaatccatc ctgactcagc aaatcttttg
    23881 ggtgaacagt aaccgtccaa tggactggga ctggatcaaa gcgttcccgc aaagcctgaa
    23941 agatgaattt aagtcgatga aaatcacggt gaactgggaa aaagcctggc ctgccgtttt
    24001 tatcgctttc ctcgccggtt tgccgctgct gttgattgcc gggctgatcc actggcgtct
    24061 gggctggctg aaagcgtatc aacagaaact ggcttccgct gtgggttccc tgcgtaacga
    24121 tagccagctc aacacaccaa aagcgatcct tatcgacctg atccgcgcgc tgccggtgtg
    24181 cctgattatt ctcgcggttg gcctgattct gttgaccatg cagctcaaca tcagcgaact
    24241 gctatggtcg ttcagtaaaa aactggcgat tttctggctg gtgtttggcc tgtgctggaa
    24301 ggtactggag aaaaatggcg ttgccgtgcg tcacttcggc atgccggagc agcagaccag
    24361 ccactggcgt cggcaaattg tccgcatcag tctcgcgttg ctgcctatcc atttctggtc
    24421 tgtggtggca gaactttccc cgctgcatct gatggatgat gtgctggggc aggcgatgat
    24481 tttcttcaac ctgctgctga ttgccttcct ggtatggcca atgtgccgcg aaagctggcg
    24541 tgataaagag tcgcacacca tgcgactggt caccattacc gtgctgtcga ttatcccgat
    24601 tgcattgatg gtgctgactg ccacgggtta tttctacacc acgctgcgcc tctccgggcg
    24661 ttggattgaa accgtttatc tggtgatcat ctggaacttg ctctaccaga cggtattgcg
    24721 cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt gcgctggcgc gtcggcagaa
    24781 tctggtgaaa gagggcgcag aaggtgccga accgccggaa gaacccacca ttgcactgga
    24841 acaggttaac cagcagacgc tgcgtattac catgttgctg atgtttgcgc tgttcggtgt
    24901 catgttctgg gcaatttggt ccgatttgat caccgtgttc agctatctcg acagcatcac
    24961 gctctggcat tacaacggca ctgaagctgg cgctgcggtg gtgaaaaacg tcaccatggg
    25021 cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg gcgttgattc gcaacctgcc
    25081 tggtttactg gaagtgctgg tgctctcgcg actgaatatg cgtcagggcg cgtcgtatgc
    25141 cattactacc atccttaact acatcattat tgccgttggt gcgatgacgg tgttcggatc
    25201 gctgggcgtc tcttgggata aactccagtg gctggccgca gcattatccg taggtcttgg
    25261 ttttggttta caagaaattt tcggtaactt cgtctccggt ttgatcattc tgttcgaacg
    25321 tccggtgcgt attggcgata cggtaaccat tggtagcttc tcggggacgg taagtaagat
    25381 ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa gaagtgatca tcccgaacaa
    25441 agcgtttgtt accgagcgtc tgatcaactg gtcgttgact gacactacta cgcgtctggt
    25501 gatccgtctc ggcgtggcct atggctccga tctggaaaaa gtgcgtaaag tgttactgaa
    25561 ggcggcgact gagcacccaa gggtgatgca cgaaccaatg ccggaagtct tctttacggc
    25621 gtttggtgcc agcacgttgg atcatgagct gcgtctgtat gtgcgtgaac tgcgtgaccg
    25681 tagtcgtact gtcgatgagc tgaaccgtac tatcgatcag ctgtgccgtg aaaacgacat
    25741 caacattgcc tttaaccagc ttgaagtgca tctgcacaac gagaagggcg atgaggtgac
    25801 ggaagtcaaa cgcgactaca aaggcgatga cccgacgcca gcggtagggt aataaccaaa
    25861 gggggcgaca tttagttgcc ccgagattac tgacaaagtg cgcgttgttc atgccggatg
    25921 cggcgtgacc gccttatccg gcctacgaaa ccgcaagaat tcaatgtatt gcaggagcgg
    25981 tgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgcccacaa tcttagggga
    26041 catttagcgg ccccatttat ttctcacttt tccgccttat catcgcgcgt taatttcttt
    26101 tcataatcgc gttttacaat atccagcgcg cgcagcacgg tgcgggctgg gatctgattt
    26161 tcttccagca gcacaatcaa atcgacagcc agtttgacat cgtcaggggc attttcaagt
    26221 gacatactct ctccattgct agcgggttaa gcgcgctagc ctgttttcga ttttttccag
    26281 cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc acttcacgat gcagcgtttg
    26341 ctgttccacg agatcggtca cccgcgccag gcgggctctg cgttcggcaa ccatctcacg
    26401 tagccgccgc tcaaaatcct gatgctgaat acgtttacgc tgccagcggg caattttcgg
    26461 tggcgcactg tcccactcgc gcaatgacca ggcggaggct tcacgcgcga tggcttccag
    26521 ttgtgccgcc agatgttccg ccagccaggc cacttgcggc agctgttgct gctcaaccgc
    26581 atgacaaagc gcagccagat tatcgcccgc ctcgtcgaga caagcctgta gtgttgtcgc
    26641 acgagtctga aaaagatgcc tgtcgaaacg agcacttagc gtggcgaact gtgacaccgg
    26701 ggcacaacgc tgacgcagcg tagcgagctg tccttccagt ttttccagca gcagggcggt
    26761 tttcacgata agacctcaat gaaaatgata attgttatgc taaagtagcc actctgtaag
    26821 ctgacaacac tcaatatgca acgaatcatt ttaatcatca ttggctggct ggcggtagtg
    26881 ctgggtacgc tgggcgtggt attaccggta ttaccgacga cgccgtttat cctgctggcg
    26941 gcctggtgct ttgcccgttc ttccccgcgc tttcacgcct ggttgctgta ccgctcatgg
    27001 tttggcagct atctacgttt ctggcagaaa catcatgcga tgccgcgcgg cgtcaaaccg
    27061 cgggcgattt tgcttatttt gctcacgttt gccatttctc tgtggttcgt ccagatgcca
    27121 tgggtgcgca tcatgttgct ggtaattctc gcctgtttgc ttttctatat gtggcgaatt
    27181 ccggtgattg atgaaaagca agaaaagcac tgaagcacaa caatcgcagt tgcaattatt
    27241 gcgtacagcc agtacattct ggcgttttcg agcacaggcg caggcggtca aaggttaaac
    27301 aactgttact tttgatacgt ttaaaacgcg ccgtgagtac caccgtaaca agcaggcata
    27361 cacttatgac cgcgactgca cagcagcttg agtatctcaa aaatagcatc aaaagcattc
    27421 aggactaccc aaaacccggc attcttttcc gcgatgtcac cagcttactg gaagacccga
    27481 aagcttacgc tctcagcatc gacttgctgg ttgagcgtta caaaaatgcg ggcattacca
    27541 aagttgtcgg caccgaagcg cgtggcttct tgtttggcgc tccggtagct ctgggtctgg
    27601 gcgttggctt tgtaccggtc cgtaaaccgg gcaaactgcc gcgtgaaacc atcagtgaaa
    27661 cttacgacct ggaatacggc accgatcagc tggagatcca cgttgatgcc atcaaaccgg
    27721 gcgacaaagt tctggtggtg gacgatctgc tggcaaccgg cggcactatc gaagcgaccg
    27781 ttaaactgat ccgtcgtctg ggtggtgaag tggctgacgc tgcgttcatt atcaacctgt
    27841 tcgatctcgg cggcgaacag cgtctcgaaa aacagggcat taccagctac agccttgtcc
    27901 cgttcccggg ccattaatta tcgccagtct tgtgctgccc acgctacgga cagcacaaga
    27961 tgtgcattca gcctcgccgt tctgacgggg ctgtgttagc attacccctt cgtgaatcca
    28021 ccttccagcg tttcagagcc tgccaatgag ttatcaggtc ttagcccgaa aatggcgccc
    28081 acaaaccttt gctgacgtcg tcggccagga acatgtgctg accgcactgg cgaacggctt
    28141 gtcgttaggg cgtattcatc atgcttatct tttttccggc acccgtggcg tcggaaaaac
    28201 ctctatcgcc cgactgctgg cgaaggggct aaactgcgaa accggcatta ccgcgacgcc
    28261 gtgcggcgtg tgcgataact gtcgtgaaat cgagcagggg cgctttgtcg atctgattga
    28321 aatcgacgcc gcctcgcgca ccaaagttga agatacccgc gacctgctgg ataacgtcca
    28381 gtacgctccg gcgcgtggtc gtttcaaagt ttatctgatc gacgaagtgc atatgctgtc
    28441 gcgccacagc tttaacgcac tgttaaaaac ccttgaagag ccgccggagc acgttaagtt
    28501 tctgctggcg acgaccgatc cacagaaatt gccggtgacg attttgtcgc gctgtttgca
    28561 atttcatctc aaggcgctgg atgtcgagca aattcgccat caacttgagc acatcctcaa
    28621 cgaagaacat atcgctcacg agccgcgggc gttgcaattg ctggcgcgcg ccgctgaagg
    28681 cagcctgcga gatgccttaa gtctgaccga ccaggcgatt gccagcggtg acggccaggt
    28741 ttcaacccag gcagtcagtg cgatgctggg tacgcttgat gacgatcagg cgctgtcgct
    28801 ggttgaagcg atggtcgagg ccaacggcga gcgcgtaatg gcgctgatta atgaagccgc
    28861 tgcccgtggt atcgagtggg aagcgttgct ggtggaaatg ctcggcctgt tgcatcgtat
    28921 tgcgatggta caactttcgc ctgctgcact tggcaacgac atggccgcca ttgagctgcg
    28981 gatgcgtgaa ctggcgcgca ccataccgcc gacggatatt cagctttact atcagacgct
    29041 gttgattggt cgcaaagaat taccgtatgc gccggaccgc cgcatgggcg ttgagatgac
    29101 gctgctgcgc gcgctggcgt tccatccgcg tatgccgctg cctgagccag aagtgccacg
    29161 ccagtccttt gcacccgtcg tgccaacggc agtaatgacg ccaacccagg tgccgccgca
    29221 atcagcgccg cagcaggcac cgactgtacc gctcccggaa accaccagcc aggtgctggc
    29281 ggcgcgccag cagttgcagt gcgtgcaggg agcaaccaaa gcaaaaaaga gtgaatcggc
    29341 agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg gaaagactgg cttcggtcac
    29401 cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa aaagcgtcag ccaaaaaaga
    29461 agcgtatcgc tggaaggcga ccactccggt gatgcagcaa aaagaagtgg tcgccacgcc
    29521 gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg gaactggcgg cgaagctagc
    29581 ggcagaagcc attgagcgcg acccgtgggc ggcacaggtg agccaacttt cgctaccaaa
    29641 actggtcgaa caggtggcgt taaatgcctg gaaagaggag agcgacaacg cagtatgtct
    29701 gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt gcacagcaaa aactggctga
    29761 agcgttgagc atgttaaaag gttcaacggt tgaactgact atcgttgaag atgataatcc
    29821 cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac gaagaaaaac ttgcgcaggc
    29881 gcgcgagtcc attattgcgg ataataatat tcagaccctg cgtcggtttt tcgatgcgga
    29941 gctggatgaa gaaagtatcc gccccatttg atcgtaagca cagcttacgt tcgtcatcct
    30001 taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg tctgggtaac ctgatgaagc
    30061 aagcccagca gatgcaagaa aaaatgcaga aaatgcagga agagatcgcg cagctggaag
    30121 tcaccggcga atctggcgca ggtctggtaa aagtgaccat caacggtgca cacaactgcc
    30181 gtcgcgtaga gatcgatccg agcctgctgg aagacgacaa agagatgctg gaagacctgg
    30241 tggctgcagc attcaacgac gcagcacgtc gtattgaaga aacgcagaaa gaaaaaatgg
    30301 cctctgtatc ctccggaatg cagctgccgc ctggctttaa gatgccgttc tgatgcaaac
    30361 cagcccgctg ttaacacagc ttatggaagc actgcgctgt ctgccgggcg ttggcccgaa
    30421 gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat cgtagcggcg ggatgcgttt
    30481 ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac tgcgccgatt gccgcacttt
    30541 caccgaacag gaagtctgta acatctgttc gaatccgcgt cgtcaggaaa acggtcaaat
    30601 ctgtgtggtg gagagtccgg cggacatcta cgccattgag cagacggggc agttttctgg
    30661 tcgttatttt gtgttgatgg ggcatctgtc accgctggac ggcatcggtc cggatgatat
    30721 cgggcttgat cgtctggaac agcgtctggc agaggaaaaa atcactgaag tgatcctcgc
    30781 caccaatccc acggttgaag gtgaagctac cgctaactac attgccgagc tttgcgcgca
    30841 atatgacgtg gaagccagcc gaatcgctca tggcgtaccg gttggcggcg aactggaaat
    30901 ggtcgatggc accacgctgt cacactccct tgccgggcgt cataagattc gtttttaagc
    30961 aaacgagagc aggatcacct gctctcgctt gaaattattc tcccttgtcc ccatctctcc
    31021 ctcatcctgt ttttaacctt aaaatggcat tattgaggta gacctacatg aaaggacaag
    31081 aaactcgtgg ttttcagtca gaagtgaaac agcttctgca cctgatgatc cattctctct
    31141 attccaataa agaaatcttc ctgcgtgagc ttatctctaa cgcctccgat gcggcggaca
    31201 agctgcgttt ccgtgcgcta tctaacccgg acctgtacga aggtgatggc gaactgcgcg
    31261 ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat ctctgataac ggcgtgggga
    31321 tgacccgcga cgaagtgatt gaccatctgg ggactatcgc taaatccggt accaaatcat
    31381 tcctcgaatc cctgggttct gaccaggcga aagacagcca gctgatcggt cagtttggtg
    31441 ttggtttcta ctctgcgttt atcgtggccg acaaagtgac cgtgcgtact cgcgcggcag
    31501 gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg cgaaggtgaa tataccgttg
    31561 ccgacatcac caaagaagat cgtggtactg aaatcaccct gcatctgcgt gaaggcgaag
    31621 acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag caaatactcc gaccatatcg
    31681 cgctgccggt agagatcgaa aaacgcgaag agaaagacgg cgaaaccgtt atctcctggg
    31741 agaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa gtcggaaatc accgatgaag
    31801 agtacaaaga gttctacaaa cacatcgccc acgactttaa tgatccgctg acctggagcc
    31861 acaaccgcgt tgaaggtaag caggagtaca ccagcctgct gtacattccg tcccaggctc
    31921 cgtgggatat gtggaaccgt gatcataaac acggcctgaa actgtacgtc cagcgtgtgt
    31981 tcatcatgga cgacgcagaa cagttcatgc cgaactatct gcgcttcgtg cgtggtctga
    32041 ttgactccag cgatctgccg ctgaacgttt cccgtgaaat cctccaggac agcacggtaa
    32101 cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca aatgctggaa aaactggcga
    32161 aagacgacgc ggaaaaatac cagaccttct ggcaacagtt tggcctggta ctgaaagaag
    32221 gtccggcgga agatttcgct aaccaggaag cgatcgccaa actgctgcgt tttgcttcta
    32281 cccataccga ttcttctgcg cagaccgtat ctctggaaga ctacgtttcc cgcatgaaag
    32341 aagggcagga gaaaatctac tacatcaccg cagacagcta tgcggcagcg aagagcagcc
    32401 cgcacctgga actgctgcgt aagaaaggca tcgaagttct gctgctttcc gaccgcatcg
    32461 atgagtggat gatgaactat ctgactgagt tcgacggtaa accgttccag tcggtgtcta
    32521 aagttgacga gtcgctggaa aaactggctg acgaagttga tgagagcgcg aaagaagcgg
    32581 agaaagcact gactccgttc atcgaccgtg tgaaagccct gctcggcgag cgcgtgaaag
    32641 atgtccgtct gactcaccgt ctgaccgata cgccagcgat cgtttcgacc gacgcggacg
    32701 aaatgagcac tcagatggcg aaactgttcg ctgcggcggg ccagaaagtg ccagaagtga
    32761 aatacatctt cgaactgaac ccggatcacg tactggtgaa acgtgcggca gatactgaag
    32821 atgaagcgaa gttcagcgag tgggtagaac tgctgctgga tcaggcgctg ctggcagaac
    32881 gcggcacgct ggaagatccg aacctgttta ttcgtcgtat gaaccagctg ctggtttcct
    32941 gatgtcatgc cggatgacct ttgtgtcatc cggcattttt cttttcatca tctgcacttt
    33001 ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg cctttcttga ggcaatcgcc
    33061 tgttggtggt atcgtttatc gctttttcaa aaaaatcgac acattttaag gggattttcg
    33121 caatgcgtat cattctgctt ggcgctccgg gcgcggggaa agggactcag gctcagttca
    33181 tcatggagaa atatggtatt ccgcaaatct ccactggcga tatgctgcgt gctgcggtca
    33241 aatctggctc cgagctgggt aaacaagcaa aagacattat ggatgctggc aaactggtca
    33301 ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc tcaggaagac tgccgtaatg
    33361 gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc agacgcgatg aaagaagcgg
    33421 gcatcaatgt tgattacgtt ctggaattcg acgtaccgga cgaactgatt gttgatcgta
    33481 tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta tcacgttaaa ttcaatccgc
    33541 cgaaagtaga aggcaaagac gacgttaccg gtgaagaact gactacccgt aaagacgatc
    33601 aggaagaaac cgtgcgtaaa cgtctggttg aataccatca gatgactgca ccgctgatcg
    33661 gctactactt caaagaagcg gaagcgggta acaccaaata cgcgaaagtt gacggcacca
    33721 agtcggttgc tgaagttcgc gctgatctgg aaaaaatcct cggctaatca agccgcagca
    33781 gatctgcttt cgggcaggtc tgctcatcca cctcaggcaa tacctctcac agcaattagt
    33841 tcttcttcct cacttttccg ctacaattat caacaagttg aatcgataag aggcggtaat
    33901 gcgtcagact aaaatcggta tcctgctggc aaacctgggt acgcccgatg cccccacacc
    33961 tgaagcggta aaacgctatc tgaaacaatt tttaagcgac agacgcgtgg ttgatacctc
    34021 acggttgtta tggtggccat tgctgcgcgg cgttattttg ccgctgcgct cgccgcgtgt
    34081 ggcgaagctg tacgcctcgg tctggatgga aggtggctcg ccgctgatgg tttacagccg
    34141 ccagcaacag caggcgctgg cacaacgttt accggagacg cccgtagcgc tgggaatgag
    34201 ctacggctcg ccatcactgg aaagcgccgt cgatgaactc ctggcagagc atgtagatca
    34261 tattgtggtg ctgccgcttt atccgcaatt ctcctgttct acggtcggtg cggtatggga
    34321 tgaactggca cgcattctgg cgcgcaaacg tagcattccg gggatatcgt ttattcgtga
    34381 ttacgccgat aaccacgatt acattaatgc actggcgaac agcgtacgcg cttcttttgc
    34441 caaacatggc gaaccggatc tgctgctgct ctcttatcat ggcattcccc agcgttatgc
    34501 agatgaaggc gatgattacc cgcaacgttg ccgcacaacg actcgcgaac tggcttccgc
    34561 actggggatg gcaccggaaa aagtgatgat gacctttcag tcgcgctttg gccgggaacc
    34621 ctggctgatg ccttataccg acgaaacgct gaaaatgctc ggagaaaaag gcgtaggtca
    34681 tatacaggtg atgtgcccgg gctttgctgc ggattgtctg gagacgctgg aagagattgc
    34741 cgagcaaaac cgtgaggtct tcctcggtgc gggcgggaaa aaatatgaat atattccagc
    34801 gcttaatgcc acgccggaac atatcgaaat gatggctaat cttgttgccg cgtatcgcta
    34861 aagctgagcg gtaaagaact gagcgccgtc gcgaagagcc tcgtcggcgg ttttcatcat
    34921 ccgtgaataa tgcaaaaagg catgcagcgt acctgggtag agtttgaact cacagggctg
    34981 ctgatgcgcc gctaacgtct ggtaaagcag acggctgtca tccagcagcg gatcgaactc
    35041 cgccccggca ataaaacagg gcggaacttc gcgagtgaga tcattattaa acagacagta
    35101 atacggtgac tcgcggtccg cgtcgttgct taaatacgcc tcttcataca tctgcaaatc
    35161 ctgttgcgtt aagccatccc agacaccgcc caacagacga cgagtcacgg aatcccgtaa
    35221 tccgtaaagc ccataccaca gcaaaacacc cgcaatttta ccgcaatcga tctgtttatc
    35281 acgcaaccac aacgcactgg cgagcgccag catggcacct gcggaatcac cggcaaagcc
    35341 aatgcgggac atattgattt gataatcctc cgcctgctgg tggaaataac aacaagcagc
    35401 cacaatttcc tctatcgctt gcggaaaacg cgcttcaggt gaaagggtgt aatcaatacc
    35461 aatcaccgta cattggctgt agcttgccag caggcgcatg atgcgatcgt gggtatcgag
    35521 attgccgaga ataaaaccgc ctccatgcaa ataaaatagc gtcgccgggc tatctggctg
    35581 cggacaaaag agacgtgttt ccacctgccc atattttgtt ggaaccatgt aagctctggt
    35641 tgccatttct ggagcgcccg cattccagaa tcggcgctca agcgtgtaat actgtcgttg
    35701 ctcagcaatc gttcccgttg cgggccagga cggtaaatcc ggctgaagag tattcacaac
    35761 ggtcttcatt tcagcagaaa taaggtccag aacaggtagt ttgttttccg gcttcataca
    35821 aaaactcctt tcaaattacg tcattgtaag gaaccactgc catgaaaatg cgatcccgcc
    35881 tgctgatatt gaaactggct gcgtctcgcg cgctcccgtc agattgtgtt aacattcgcc
    35941 gctcagttaa ccacccgtaa aaacaaccat gaaatttccc ggtaaacgta aatccaaaca
    36001 ttacttccct gtaaatgcac gcgatccgct gcttcagcaa ttccagccag aaaacgaaac
    36061 cagcgccgcc tgggtcgtag gtattgacca gacgctggtc gatattgaag cgaaagtgga
    36121 tgatgaattc attgagcgtt atggattaag cgccgggcat tcactggtga ttgaggatga
    36181 cgtagccgaa gcgctttatc aggagctaaa acagaaaaac ctgattaccc atcagtttgc
    36241 gggtggcacc attggtaaca ccatgcacaa ctactcggtg ctcgcggacg accgttcggt
    36301 gctgctgggc gtcatgtgca gcaatattga aattggcagt tatgcctatc gttacctgtg
    36361 taacacttcc agccgtaccg atcttaacta tctacaaggc gtggatggcc cgattggtcg
    36421 ttgctttacg ctgattggcg agtccgggga acgtaccttt gctatcagtc caggccacat
    36481 gaaccagctg cgggctgaaa gcattccgga agatgtgatt gccggagcct cggcactggt
    36541 tctcacctct tatctggtgc gttgcaaacc gggtgaaccc atgccggaag caaccatgaa
    36601 agccattgag tacgcgaaga aatataacgt accggtggtg ctgacgctgg gcaccaagtt
    36661 tgtcattgcc gagaatccgc agtggtggca gcaattcctc aaagatcacg tctctatcct
    36721 tgcgatgaac gaagatgaag ccgaagcgtt gaccggagaa agcgatccgt tgttggcatc
    36781 tgacaaggcg ctggactggg tagatctggt gctgtgcacc gccgggccaa tcggcttgta
    36841 tatggcgggc tttaccgaag acgaagcgaa acgtaaaacc cagcatccgc tgctgccggg
    36901 cgctatagcg gaattcaacc agtatgagtt tagccgcgcc atgcgccaca aggattgcca
    36961 gaatccgctg cgtgtctatt cgcacattgc gccgtacatg ggcgggccgg aaaaaatcat
    37021 gaacactaat ggagcggggg atggcgcatt ggcagcgttg ctgcatgaca ttaccgccaa
    37081 cagctaccat cgtagcaacg taccaaactc cagcaaacat aaattcacct ggttaactta
    37141 ttcatcgtta gcgcaggtgt gtaaatatgc taaccgtgtg agctatcagg tactgaacca
    37201 gcattcacct cgtttaacgc gcggcttgcc ggagcgtgaa gacagcctgg aagagtctta
    37261 ctgggatcgt taagttatcg tcggttcgta ggccagataa ggcgttcacg ccgcatctgg
    37321 catttggctc tcgatgcctg atgcgacgct ggcgcgtctt atcatgccta catatttttc
    37381 atattttaca tccggcaacc accgtttacc ccgtcaccac ttcacccgcc ggtggcgttt
    37441 ccagcagttc cagcatagta cgggcaattt cacgttcacc catcactacc tgattcgcgc
    37501 cacgttcggt gatatacgtc acttcatcgt cataatgggc gcgggcgata atctcaatat
    37561 ccgggttttt cgcgcgggca gacgccacaa tctcacccgc ttcataaccg ttggggatcg
    37621 tcaggatcag ccattttgca cattccagat gcgccagttg cataatttct tcgttcgctg
    37681 cattgcccaa tacagcgcgg accccacgct ctcgcagctc atcaacacgg gttcgtgacg
    37741 tctcaatcac caccagcgga atatcagagg cgagcaattt ctcccccagc aggctgccta
    37801 cacgaccgta acccaccaat agcgcatggt tgcaaatatc cactgggatc tgtttctctt
    37861 cttcgattgc ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc agatatttct
    37921 ccagtagtgc gaacagtacc gggttgagca taatcgacag gatcgcccct gccagtacca
    37981 ggttttgtcc ggcctgcggc agtaaattca atgccattcc cagtcccgcc aggataaacg
    38041 cgaactcacc aatctgcgcc aggctggcgg cgatggttaa tgccgtacgt tgggagtgac
    38101 caaacagacg gacaaggaac agggccgcga ccgatttacc aaacagaata attgccagcg
    38161 tcgccagcac tgccagcggt tgctgaatca gaattaacgg atcaaacaac atcccgacgg
    38221 agacaaaaaa cagcaccgca aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt
    38281 gactcagttc agactcgttc agtaccatcc cggcaaagaa cgcgccaagt gcgaaggaga
    38341 cgtcaaacaa ctccaccgca ccaaaggcaa tccctaacgc cagcgccagc accgacaggg
    38401 taaatagctc gcgagaaccg gttgccgcgc tgcgtgccat aatccacggc accagacggc
    38461 gacctaccag catcataatg gcgataaatg cgatcacttt gccgatggtg atccccatat
    38521 cgactgcaag agtggcaaag cccacatcgc cctgttccat cattcctgcc actgcgggca
    38581 gcaacaccag cgtcagaacc attaccaggt cttccacaat caaccaaccg atggcgattt
    38641 gcccacgctg actgtcaatt aattgccgtt cttcaagtgc gcgcagtaac accacggtac
    38701 tggcggtgga aagacataaa ccgaacacga taccggtcat taacgaccag cccagcacgg
    38761 cagagagcgc catacccagc agcgtcgcca cggctatctg ggcgatcgca ccgggaatgg
    38821 cgatggcctt taccgccatc aaatccttca gcgaaaagtg caaaccgacg ccaaacatca
    38881 acagaatgac gcccagttca gccagttccg gcgcaagctt ggtatcggca acaaagcccg
    38941 gagtgaatgg tcctgccagc acacccgcta acagatatcc caccagagga gaaatacgta
    39001 gtttattggc cagcatgccg aggataaagg cgagcacaag gccgccaaca atggtggtga
    39061 taagcggggt ggcgtgatgc attccgtctc cttttcctgg tggttattgt ccatttttgg
    39121 ccgggaaaac caaaattaca ggtaatagtt tatgacaatt tcattgatga tgttcatgaa
    39181 taattattga attttgcaga aaaatggaat tagctgcaaa aaaagcacgg atcggaaaac
    39241 agaaggcggt ttaacagagg aaaggcttat ggcgactgta aaacgatgca gccaaagttt
    39301 gcctttggct gcaatgaaat cagtctttat gccggttatc aggcaggaat atggtcaaca
    39361 tccccaatag tggcaggaaa gcacagattt tatagactaa ctcgatg
//
LOCUS       DAJVRF010000021        39259 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJVRF010000021 DAJVRF010000000
VERSION     DAJVRF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39259
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.021"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="OQN13_001005"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="OQN13_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCS1614429.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="OQN13_001006"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="OQN13_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCS1614430.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="OQN13_001007"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="OQN13_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCS1614431.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="OQN13_001008"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="OQN13_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCS1614432.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="OQN13_001009"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="OQN13_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCS1614433.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDISARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLVQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="OQN13_001010"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="OQN13_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCS1614434.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="OQN13_001011"
     CDS             complement(6574..7338)
                     /locus_tag="OQN13_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1614435.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="OQN13_001012"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="OQN13_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCS1614436.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="OQN13_001013"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="OQN13_001013"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCS1614437.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="OQN13_001014"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="OQN13_001014"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCS1614438.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="OQN13_001015"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="OQN13_001015"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCS1614439.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="OQN13_001016"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="OQN13_001016"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCS1614440.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="OQN13_001017"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="OQN13_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCS1614441.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRMEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="OQN13_001018"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="OQN13_001018"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCS1614442.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="OQN13_001019"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="OQN13_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCS1614443.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="OQN13_001020"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="OQN13_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCS1614444.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="OQN13_001021"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="OQN13_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCS1614445.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="OQN13_001022"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="OQN13_001022"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCS1614446.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALREYVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="OQN13_001023"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="OQN13_001023"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCS1614447.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="OQN13_001024"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="OQN13_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCS1614448.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="OQN13_001025"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="OQN13_001025"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCS1614449.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="OQN13_001026"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="OQN13_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCS1614450.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="OQN13_001027"
     CDS             complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="OQN13_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCS1614451.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="OQN13_001028"
     CDS             complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="OQN13_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCS1614452.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(30043..31374)
                     /locus_tag="OQN13_001029"
                     /pseudo
     CDS             complement(30043..31374)
                     /locus_tag="OQN13_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="OQN13_001030"
     CDS             complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="OQN13_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCS1614453.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31717..33003)
                     /locus_tag="OQN13_001031"
     CDS             complement(31717..33003)
                     /locus_tag="OQN13_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1614454.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(33054..33995)
                     /locus_tag="OQN13_001032"
     CDS             complement(33054..33995)
                     /locus_tag="OQN13_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCS1614455.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(34010..34780)
                     /locus_tag="OQN13_001033"
     CDS             complement(34010..34780)
                     /locus_tag="OQN13_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCS1614456.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35253..36767
                     /gene="caiT"
                     /locus_tag="OQN13_001034"
     CDS             35253..36767
                     /gene="caiT"
                     /locus_tag="OQN13_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCS1614457.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36798..37940
                     /gene="caiA"
                     /locus_tag="OQN13_001035"
     CDS             36798..37940
                     /gene="caiA"
                     /locus_tag="OQN13_001035"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCS1614458.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            38069..39259
                     /gene="caiB"
                     /locus_tag="OQN13_001036"
                     /pseudo
     CDS             38069..39259
                     /gene="caiB"
                     /locus_tag="OQN13_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
BASE COUNT         9396 a        10791 c        10280 g         8792 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatctccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggtg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caagagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaatgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggta aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtatg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctgggg gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatttcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgtcg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tatgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcaacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaagca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gtcagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcccg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa atggttcgat
    28621 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    28681 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    28741 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    28801 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc
    28861 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta gcggaatcat
    28921 cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc
    28981 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga
    29041 gagcgccagc gtcatgccga tcaattccgc gacctgccag tgcagcccaa gtaacatgca
    29101 gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    29161 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    29221 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    29281 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    29341 taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct gaatcagcgt
    29401 atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct
    29461 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    29521 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    29581 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    29641 atttgccatg cagcgccttg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    29701 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcggatgc tgccagacga
    29761 tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    29821 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    29881 gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta
    29941 gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    30001 agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca ttctctgccc
    30061 gcggatcgtc atattgctgg tttgcgccag tgacatccct cgagtctccg gggcaaacgc
    30121 tacggaaatc aacaagccaa acagcgagat acccgcctcc attagcatcg tgttactgat
    30181 accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc caatacggct
    30241 taaggacata atcacgccca cggcagaggc gcggatatct gtcgggaaga gttcattagg
    30301 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    30361 cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat
    30421 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttaacc acagcatcgg
    30481 cggaatacag ccgagcataa agaacaggct aatcaccacg ttccccagcg ccgcgttttt
    30541 gccaacaccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    30601 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    30661 attaaacaga tcgcgaaaac gggtttgcta cggctgctct tcatcgaaag ccaccggttc
    30721 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    30781 gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgccgc
    30841 gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc cgaccagatc
    30901 ggcgcaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa atgcccgctg
    30961 acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg caccgatgac
    31021 aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa acatcgtcgc
    31081 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtcctacttt
    31141 atcggaaata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    31201 caacaagcca atccagtcag cgtccagttt tagcgccgga gtcagttgct ccagcgccac
    31261 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    31321 gcggcggtga attgaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    31381 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    31441 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    31501 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    31561 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagggcct
    31621 gtttatcggc atcggccttt acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    31681 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    31741 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    31801 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    31861 ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag gccggtagtt tctggtacat
    31921 acgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt
    31981 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc
    32041 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    32101 accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc ctgcttccgg
    32161 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg ccactgccgg
    32221 atgctgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg
    32281 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    32341 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    32401 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    32461 cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga tggagttcac
    32521 tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    32581 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    32641 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa cggagtaaga
    32701 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    32761 cgtcataaac gcgagttttt catgggtgat caggcgttct accggggcgg attcggcgaa
    32821 accaggaata atgtgttcca ggctgtgggc atagagacgc ccgccggtga cgttcttggc
    32881 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    32941 tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga
    33001 cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc
    33061 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc
    33121 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca
    33181 ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt
    33241 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    33301 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    33361 gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg
    33421 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    33481 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    33541 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    33601 gccagaccac cgtaaaccat atgtttcact gttgctttac cgtcctgtac gctgacggtg
    33661 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    33721 ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    33781 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    33841 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg
    33901 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    33961 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    34021 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    34081 gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    34141 taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg ccgagaatgg
    34201 ctttcatcga aggaatttgt ggggaattga tatcagtgga aacagcaaca accgcaggca
    34261 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    34321 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    34381 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    34441 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    34501 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agtacatctt
    34561 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct
    34621 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta
    34681 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    34741 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    34801 aggaaacatc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    34861 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    34921 gttaagttcc tcaccaatat tgaaaacatg gcgtagaaaa aagaaatttt caatattgct
    34981 ttatggatca ccaatattga aagtcagatg ctattttttt actcacaaca caacataaca
    35041 aactgattaa taatcaaaat taacgagaaa agcgccctct attcacccga aaaaaaatgt
    35101 gataacaatc acagaataca gcttattgaa tacccattat gagttagcca ttaacgcgtc
    35161 cacgaggtta ataataatta tatcaaatgt taacaaaaat aaaacaaacg ggaacgcaaa
    35221 ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa
    35281 tagaaccgaa ggttttcttt ccgccgttaa taatcgtcgg catactttgt tggcttacag
    35341 tcagagatct ggatgcagcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg
    35401 tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg
    35461 tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc acctgaattt agtaccgcca
    35521 gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga
    35581 ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacagggg
    35641 cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt
    35701 acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc
    35761 gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggca
    35821 ctatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc
    35881 ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc
    35941 aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg
    36001 gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc
    36061 tgggttgggt gttcattgtc agcggtgcca gcttcatcat gaactacttc accgattcgg
    36121 tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag
    36181 gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt tatgctatcc
    36241 agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg
    36301 gcatggtgct ggggctgaca gcgtcaacct ggatcctgtg gactgtactc ggtagtaaca
    36361 ctctgctgtt gatagataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg
    36421 tggcgcgcgc catcattgaa acctgggccg ctctgccact cagcaccgcc accatgtggg
    36481 gcttcttcat cctctgcttt attgccaccg ttacactggt taacgcctgc tcttataccc
    36541 tggcgatgtc cacttgccgc gaagtacgcg atggtgaaga accacctctg ctggtgcgta
    36601 tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga
    36661 aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg
    36721 tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa
    36781 tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg
    36841 gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg
    36901 acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc
    36961 tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga
    37021 tggagctggg acgtctgggg gcaccaacct atgtgctgta ccagttgccg ggcgggttca
    37081 acaccttcct gcgcgaaggc acacaagagc agatcgacaa aattatggct ttccgcggca
    37141 ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta
    37201 gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt
    37261 ttattaccag cagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc
    37321 cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga
    37381 ccaaacttga aaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgacg
    37441 tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag
    37501 aagagttcga ccatgaacgt ttcctggtag ccctcaccaa ctacggtacg gcgatgtgcg
    37561 cttttgaaga tgcggcgcgc tacgccaatc agcgcgtgca gtttggcgag gctattggtc
    37621 gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa
    37681 acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag
    37741 cgatgtgcaa atacttctgc gccaatgcgg catttgaagt tgtggatagc gcaatgcagg
    37801 tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgatctgc
    37861 gtgtagaccg cgtctccggg ggatctgacg aaatgcagat cctgacgctg ggtcgcgcgg
    37921 tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctaatg cgtcttatca
    37981 ggcctacggg agcacgaatg taggccggat aaagcgttta cgccgcatcc ggcagtcatg
    38041 cactacataa atttttcagg agctaataat ggatcatcta cccatgccga aattcgggcc
    38101 gttggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccgggccgt tcgccgggca
    38161 aatgttcgca gaatggggtg cggaagttat ctggatcgag aacgtcgcct aggccgacac
    38221 cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg cgctgtcgtt
    38281 aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat
    38341 cttcatcgaa gccagcaaag gtccggcctt tgcccgtcgt ggcattaccg atgaagtact
    38401 gtggcagcac aacccgaaac tggttatcgc tcacctgtcc ggttttggtc agtacggcac
    38461 cgaggagtac accaatcttc cggcctataa caccatcgcc caggccttca gtggttacct
    38521 gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg ccgattactt
    38581 ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc gtgaaaccgg
    38641 taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt
    38701 catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc
    38761 ctactacgcc ggttgcggtc tgtataaatg cgccgacggc tacatcgtga tggaactggt
    38821 gggcattacc caaattgaag agtgctttaa agatattgac ctcgcacatc tactcggtac
    38881 gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt acggcccact
    38941 ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag ttaaagaacg
    39001 ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac tggaaagcaa
    39061 tccacagtat gttgcccgtg aatcaatcac tcagtggcaa acgatggatg gtcgcacctg
    39121 caaagggccg aacatcatgc cgaaattcaa aaataacccc ggacaaatct ggcgcggaat
    39181 gccctcacat ggcatggaca cggctgccat tttgaaaaat atcggctaca gcgaaaacga
    39241 cattcaggag ttggtcagc
//
LOCUS       DAJVRF010000022        37768 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJVRF010000022 DAJVRF010000000
VERSION     DAJVRF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37768
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.022"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="OQN13_001037"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="OQN13_001037"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCS1614459.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="OQN13_001038"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="OQN13_001038"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCS1614460.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="OQN13_001039"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="OQN13_001039"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCS1614461.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="OQN13_001040"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="OQN13_001040"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCS1614462.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="OQN13_001041"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="OQN13_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCS1614463.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGVRYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="OQN13_001042"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="OQN13_001042"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCS1614464.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="OQN13_001043"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="OQN13_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCS1614465.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="OQN13_001044"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="OQN13_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCS1614466.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="OQN13_001045"
     CDS             6537..6773
                     /locus_tag="OQN13_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCS1614467.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="OQN13_001046"
     CDS             6776..7633
                     /locus_tag="OQN13_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1614468.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="OQN13_001047"
     CDS             7654..9645
                     /locus_tag="OQN13_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS1614469.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="OQN13_001048"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="OQN13_001048"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCS1614470.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="OQN13_001049"
     CDS             complement(10248..11144)
                     /locus_tag="OQN13_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCS1614471.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="OQN13_001050"
     CDS             complement(11193..11432)
                     /locus_tag="OQN13_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCS1614472.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="OQN13_001051"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="OQN13_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCS1614473.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="OQN13_001052"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="OQN13_001052"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCS1614474.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="OQN13_001053"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="OQN13_001053"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCS1614475.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="OQN13_001054"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="OQN13_001054"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCS1614476.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="OQN13_001055"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="OQN13_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="OQN13_001056"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="OQN13_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCS1614477.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="OQN13_001057"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="OQN13_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCS1614478.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESTATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="OQN13_001058"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="OQN13_001058"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCS1614479.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22739)
                     /locus_tag="OQN13_001059"
     CDS             complement(21570..22739)
                     /locus_tag="OQN13_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1614480.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="OQN13_001060"
     CDS             complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="OQN13_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCS1614481.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23293..24318
                     /gene="purR"
                     /locus_tag="OQN13_001061"
     CDS             23293..24318
                     /gene="purR"
                     /locus_tag="OQN13_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCS1614482.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24315..25247)
                     /locus_tag="OQN13_001062"
     CDS             complement(24315..25247)
                     /locus_tag="OQN13_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1614483.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25360..26571
                     /locus_tag="OQN13_001063"
     CDS             25360..26571
                     /locus_tag="OQN13_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCS1614484.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26862..28010
                     /gene="cfa"
                     /locus_tag="OQN13_001064"
     CDS             26862..28010
                     /gene="cfa"
                     /locus_tag="OQN13_001064"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCS1614485.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="OQN13_001065"
     CDS             complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="OQN13_001065"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCS1614486.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28906..30279
                     /gene="mdtK"
                     /locus_tag="OQN13_001066"
     CDS             28906..30279
                     /gene="mdtK"
                     /locus_tag="OQN13_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCS1614487.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30320..31576)
                     /locus_tag="OQN13_001067"
     CDS             complement(30320..31576)
                     /locus_tag="OQN13_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCS1614488.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31884..31960
                     /locus_tag="OQN13_001068"
     tRNA            31884..31960
                     /locus_tag="OQN13_001068"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31918..31920,aa:Val,seq:gac)
     gene            31965..32041
                     /locus_tag="OQN13_001069"
     tRNA            31965..32041
                     /locus_tag="OQN13_001069"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31999..32001,aa:Val,seq:gac)
     gene            32149..32454
                     /locus_tag="OQN13_001070"
     CDS             32149..32454
                     /locus_tag="OQN13_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCS1614489.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32580..34184
                     /locus_tag="OQN13_001071"
     CDS             32580..34184
                     /locus_tag="OQN13_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCS1614490.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34196..35008)
                     /locus_tag="OQN13_001072"
     CDS             complement(34196..35008)
                     /locus_tag="OQN13_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614491.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35012..35797)
                     /locus_tag="OQN13_001073"
     CDS             complement(35012..35797)
                     /locus_tag="OQN13_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCS1614492.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     VVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35794..36462)
                     /locus_tag="OQN13_001074"
     CDS             complement(35794..36462)
                     /locus_tag="OQN13_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCS1614493.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36526..37164)
                     /locus_tag="OQN13_001075"
     CDS             complement(36526..37164)
                     /locus_tag="OQN13_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCS1614494.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37177..>37768)
                     /locus_tag="OQN13_001076"
     CDS             complement(37177..>37768)
                     /locus_tag="OQN13_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCS1614495.1"
                     /translation="GLPLKLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLR
                     VCLHNAVTLCNWVWPMTVSPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIF
                     TLHRAYTVKLMQTKDMRNEHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYK
                     EMGWDPQLGCPTRETLQRLGLEDIAADLAAHNLLPA"
BASE COUNT         9024 a         9930 c         9380 g         9434 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcaa
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgta caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggcgg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc cattttgagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaagg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtagca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gccgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcaaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag tactgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg
    22741 ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca
    22801 gcaatcacaa aaaaatgaaa ataacaaaaa gagaaaacac ttttgccatt ttgctaacaa
    22861 acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca
    22921 gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag tgaaaattta
    22981 aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca
    23041 tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac
    23101 ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat
    23161 tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg
    23221 taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg
    23281 gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca
    23341 actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg
    23401 tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt
    23461 aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag
    23521 atcattgaag cagttgaaaa aaattgcttc cagaaaggtt ataccctgat tctgggcaat
    23581 gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc
    23641 gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag
    23701 tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat
    23761 gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc
    23821 ggtcaccgcg aaatcggcgt tatccccggc ccgctggaac gtaacactgg cgcaggccgc
    23881 cttgtcggtt ttatgaaggc gatggaagaa gcaatgatca aggtgccgga aagctggatt
    23941 gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag
    24001 ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt
    24061 gctgctgatg aaatggggct gcgcgtcccg caggatgttt cgctaattgg ttatgataac
    24121 gtgcgcaacg cccgctattt tacgccggcg ctgaccacaa tccatcagcc aaaagattcg
    24181 ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg
    24241 cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga cggcccgttc
    24301 cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc
    24361 ttattcagcg tctcactatc gccgagatac tcaagcaacc aggttaacgc aggcgacata
    24421 tcattttgct gccatgtcag acaacatgcc gaatccggaa aggggttttc cagttcaagt
    24481 gctacccact tcccctcatt aagccacggt ttggcgaaat gtgttggcac catccctatg
    24541 cataatcctg ccgagataca ggttgccgat gattcccagt caggcacgac aactcttttt
    24601 tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc
    24661 aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg
    24721 tgatggctgg caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga
    24781 ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc gtcggaaagc
    24841 gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg
    24901 cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg
    24961 atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc
    25021 atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc
    25081 acatcacggt gccgtcgttc aaagagcggc accgccagcc actcttccag ctgacgcacg
    25141 gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct aaaactacca
    25201 ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa
    25261 aatttttgaa accaatcatc aaatattacc gtttcacaac actaatttca ctccctacac
    25321 tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt
    25381 ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct
    25441 gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt
    25501 agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccgct ctccgaccgt
    25561 tatggtcgta aaccggtatt attaatcggt atgacaattt ttgcgttagg tagtctgggg
    25621 atgctgtggg tagaaaacgc cgctacgctg ctggtattgc gttttgtaca ggctgtgggt
    25681 gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg attattatcc ttcacagaaa
    25741 gttaaccgaa tttttgcgac catcataccg ctggtgggtc tatctccggc cctggctcct
    25801 ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt
    25861 gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt
    25921 aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc
    25981 aacgtgctga tatacgcggc ctgttcagcc agtttttttg catggctgac cggttcaccg
    26041 ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa
    26101 actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa atggcaaggc
    26161 aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct
    26221 gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg
    26281 attgccaatg gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacac
    26341 gcaactggtc gcgccgcagc gttgcagaac actctacaac tgggtctgtg cttcctcgca
    26401 agtctggtag tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg
    26461 atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagct
    26521 ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg
    26581 atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat
    26641 actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt
    26701 cccgactcac tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc
    26761 ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg
    26821 ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag
    26881 aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg
    26941 ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt
    27001 ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg gatggctggt
    27061 gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc
    27121 aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc
    27181 agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt
    27241 tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata
    27301 atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac
    27361 ctgggatgcg cgtactggat attggctgcg gctggggcgg actggcacac tacatggcat
    27421 ctaattatga cgtaagcgtg gtgggcgtca ccatttctgc cgaacagcaa aaaatggctc
    27481 aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg
    27541 accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg
    27601 atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggcatattc ctgctccata
    27661 ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc
    27721 cgaacggttg cctgccctct gtacgccaga ttgctcagtc cagcgaaccc cactttgtga
    27781 tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac
    27841 gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt
    27901 ttacctatta tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc
    27961 aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct
    28021 atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg attcatggca
    28081 ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatca
    28141 atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg ttccagagtt
    28201 tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata
    28261 ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact atcctggact
    28321 ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc
    28381 ataatatgac ctgacattaa gtgtccgcca atttcatcgc tgaattttgc cgcacgctca
    28441 acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc
    28501 atcaggtcaa aactgacatg gttgccgtta atttccgtca ctgtcaggca gcaaccgtta
    28561 tgcgccacgg aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca
    28621 tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc
    28681 cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca
    28741 ggtggaaaac gcccttacca gtgaaggggt aagaatgact attttttcac tggagaatta
    28801 ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag
    28861 gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc
    28921 agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa
    28981 actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg
    29041 gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg
    29101 ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg
    29161 catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg
    29221 ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc tctggcggac
    29281 aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc caggctatct tttcttccag
    29341 gttgcccgta accagtgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt
    29401 atcggcctgc tggtgaacat tccggtgaac tatatcttta tttatggtca tttcggtatg
    29461 cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggttatgttc
    29521 cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatatccg taacgaaaaa
    29581 ggcaccgcaa aacccgatcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt
    29641 gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg
    29701 ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc actaatgttc
    29761 gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag
    29821 ggctcaacgc tggatgcgca aaccgctgct cggaccgggc ttatggtggg tgtctgtatg
    29881 gcaaccctga cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac
    29941 gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag
    30001 atttctgact caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt
    30061 tccattttct atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt
    30121 ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt
    30181 attatcggcc tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt
    30241 atgccgtcag ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc
    30301 acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg
    30361 gctgtaattt cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta
    30421 tatccccgtt aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg
    30481 tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg
    30541 ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag
    30601 tgacgctgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag
    30661 taatcgttga ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta
    30721 atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt
    30781 ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg
    30841 taccggcatg cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg
    30901 aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag
    30961 tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg
    31021 ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac
    31081 ttggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa
    31141 ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac
    31201 gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt
    31261 caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct
    31321 cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac
    31381 cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga
    31441 ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg
    31501 tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt
    31561 tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa
    31621 gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa acaattctgt
    31681 ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa
    31741 atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga
    31801 aatagcgcag aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatatttg
    31861 tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac
    31921 atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt
    31981 tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc
    32041 agattttctt aatctggtct tttccttttt ccctctgttt attctctgta tccaatacgt
    32101 taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg
    32161 ttacaacttc attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa
    32221 ccacttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt
    32281 gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag cgcccttgcc
    32341 tatctggaaa aacatactgc ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa
    32401 gttttcgatg tcaatgagcc acttagtcaa atcaatcagg caaaactcgc ctgacagaat
    32461 ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa
    32521 tctcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa
    32581 tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc
    32641 tacagcaaca aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg
    32701 ggatgccata cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg
    32761 aaataccgcc gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc
    32821 tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc
    32881 tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa
    32941 aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg
    33001 gcgtcatgct cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg
    33061 ccacgggtca cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagcccgt
    33121 ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct
    33181 tgagcggact ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg
    33241 ataaacaaca cgtcgttttt caccgcgata acgcaagtga aaagctaaat atcacgttga
    33301 tgtcgcgcac gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct
    33361 tacacatcgt caccgatcag gcattaaatg ctgagattca aaaaggcgaa tatggccttt
    33421 tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc
    33481 aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc
    33541 gcaaacaacg cgacccattt gactgggcag aaaaaaatct ccaggaagtc gaacgcaata
    33601 aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac
    33661 aggaaattgt tccacatctg aatgaacacg accataaacg gttcagtaaa ggccttgccc
    33721 gggttttcat cgataattat gcggcaatcc cttcagagtc tattcgtcgg ctgctggcct
    33781 tacgtgaagc ggggatcatt catattctcg ccctcggtga agactacaaa atggaaatta
    33841 atgagtcgcg caccgtcctg aaaacggaag acaacagcta ctcgtttgac gtttttattg
    33901 atgcccgcgg acagcgtccg cttaaagtga aagatattcc tttccctggg ctacgcgagc
    33961 aattacagaa aacaggggat gaaatccctg atgttggcga agattatacg ttacagcaac
    34021 ccgaagatat tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg cacgaccagc
    34081 ctttcgttca gggacttacg gcatgtgcag aaattggtga ggcgatggct cgggcggtcg
    34141 taaagcctgc atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa agctatcagc
    34201 actcaacaaa ctgatgctgt tcattaagac gatagcggac tccggttcga atattgtttt
    34261 caccttcaat ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc
    34321 ttccccctgg cccgagaata attgagtcga ccacgcccgt gctcactacc gttgaatttt
    34381 caccgctggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg tttgcgccaa
    34441 agctggcgat ttgcaccagg tcaccattac tggcgacatg acaccgttca cccaatgtac
    34501 aaacgcgtac tcgcattccg gtattcgcaa tccgactact atctccagcg ctgcttatcc
    34561 gcgaagaatt accggttaca gcaacccgtg cgcgttcacc ggagctgcca atgtgactgt
    34621 taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgc gcggcatacc
    34681 cagcggttac gattcgggcg ttatcctcac aacagccgat gcgagcatga tcgtcgctgc
    34741 gggcaaactg actgcaaaat ggtatctctc cggggtctgt ggccagtttt tgcatagagg
    34801 aaacttcctg atgcgcgaag ttttcatccg ccagccattt tttccacgca tactcaacca
    34861 gactctccgc ccagtccgta tatccttcct caatcagtgc atgatgaata tcggcataac
    34921 taccgccgcg tgggaaatgg cgcaggaacc agcgatacat caccgctccg atacgccatt
    34981 ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca tcgaccatgc
    35041 ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatgt ccgaagataa
    35101 aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat cttacgccag
    35161 gaaacacatc tcccacggct tgcggataga gacacaacag ccccgtcaat agtaacaacg
    35221 gcagcaatcc acacatgaca ccaacatagg cgacctgctg taagggatta aatttagact
    35281 gtgttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa taaaatcgtg
    35341 tttgttttgc cgctcgttcc agccacccct gacgacgaat gcgatagtgg tgaccattat
    35401 ccccaacggc attgatcagc acaaagccga gccagcatgc cagtaacaaa aatccgcaaa
    35461 cttcatgcac cgcaaccaga cttttaacca cagttgcgcc caccatcgca aagtgattta
    35521 tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc cataatctga
    35581 ccgctttgct gtaaagataa accttctcac catgatcggt ggctgatttc ttcacgccac
    35641 gagcacgaag caaggcgtgc agccccaaca ccaaccacat cccaaccagc aatactccgg
    35701 caatgatcaa ccacaccggc caaaattcgg gagtgaactg tggcacatag ttcgctaact
    35761 ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt taatcaaatg
    35821 ttgaccgaac cggcgataca gatgcggttt cccggcacca agtagctgat attgatagta
    35881 tttattttcc tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa agatcaatgc
    35941 gtgttccgga caggcgctga cgcaaatggg cggaaagcct tttgccagtc gggactcagc
    36001 gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa cctggtacgg
    36061 acaagcgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc gcacgatccc
    36121 ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg atgcacggag catcttcgca
    36181 gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat cggtgactgg
    36241 aatgtgtgct atcgataaac ggcttccctg agcggggacg tggttagttt tacggcatgc
    36301 gcgggcgcaa atattacagc cattgcaccg tgactcgtca tgaatcatgg cataccgaac
    36361 ttccttttct tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa aaaagattac
    36421 tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat cccctaatgc
    36481 agtggctgca actgtccacc ctatccatga catctccctg aaatttcagg ggttctgaaa
    36541 ctgctctacc tcgaaccatt cgcataagcc ataagccttg ccataggtca taaatcgctc
    36601 gctaaacaag tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg catattcagg
    36661 tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt aatatggcgc
    36721 ttgcatcagc atggcgactt tgtaaggacg aggatacgtc tggcactcaa aacgaacggc
    36781 gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt
    36841 ttcactcatg gcctgcgttg agtaatagta atcatcctga cttcccgtca tacaagcgat
    36901 atccgcgtag tctccgttct gcttaatgct ttccagcaaa actgacagtt cctcttctgc
    36961 aaccgaatag ggtggcgaga gaaacacggt gcgcgccacc agctgcccag ccgcagaatg
    37021 ctggcgaatg aactcagcca ggatttcagc aggttccggt tcggttaccg tatcaacggg
    37081 gtgacgcatc ccctgcaacc attgccgctt cgcctcgtca acttcgctga ctttcgctaa
    37141 cggcaattca tcctgatggt tcatcttacc cattccttac gcaggcagta gattgtgtgc
    37201 tgccagatcg gcggcaatat cttccagccc cagccgctgc aatgtttcgc gggttggaca
    37261 accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag catgcatatc
    37321 gtcacgatcc attttgtcag taccttcagt aaagaccggg atctgcggat ccttgtcgaa
    37381 aacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca tcagttttac
    37441 cgtgtaggca cgatgcaacg taaagatacg ctctgcagct aaatctaatt tttcctgagt
    37501 catctcttcg ccggtgatcg ctttgaagaa tttggcttca agcgccagat cgccccggta
    37561 attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca gggtgacggc
    37621 gttatgcaaa cagacccgca acagcgacca ctttgcatat ttaatttttg cgtcattgat
    37681 tggcgtgtag tttttggttt catcgtaagc atcttcagaa ccaaaaagtt ctttcgccac
    37741 ttcacgttgc agtttcaatg gcaagccg
//
LOCUS       DAJVRF010000023        37449 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJVRF010000023 DAJVRF010000000
VERSION     DAJVRF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37449
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.023"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..535
                     /locus_tag="OQN13_001077"
     CDS             <1..535
                     /locus_tag="OQN13_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1614496.1"
                     /translation="KQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEA
                     LLPSLISKESPAGYKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAA
                     VWLAVASTMKEPPYVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKI
                     DSKVTNRFEVEQAIRQA"
     gene            complement(663..1154)
                     /gene="yajQ"
                     /locus_tag="OQN13_001078"
     CDS             complement(663..1154)
                     /gene="yajQ"
                     /locus_tag="OQN13_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCS1614497.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            1322..2233
                     /gene="panE"
                     /locus_tag="OQN13_001079"
     CDS             1322..2233
                     /gene="panE"
                     /locus_tag="OQN13_001079"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCS1614498.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            2196..2786
                     /gene="yajL"
                     /locus_tag="OQN13_001080"
     CDS             2196..2786
                     /gene="yajL"
                     /locus_tag="OQN13_001080"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCS1614499.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKTHEVASQLVMAAGIYNYYE"
     gene            complement(2840..4288)
                     /gene="thiI"
                     /locus_tag="OQN13_001081"
     CDS             complement(2840..4288)
                     /gene="thiI"
                     /locus_tag="OQN13_001081"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCS1614500.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            4494..4736
                     /gene="xseB"
                     /locus_tag="OQN13_001082"
     CDS             4494..4736
                     /gene="xseB"
                     /locus_tag="OQN13_001082"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCS1614501.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDTSLPPFTPDNE"
     gene            4736..5635
                     /gene="ispA"
                     /locus_tag="OQN13_001083"
     CDS             4736..5635
                     /gene="ispA"
                     /locus_tag="OQN13_001083"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCS1614502.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            5660..7522
                     /gene="dxs"
                     /locus_tag="OQN13_001084"
     CDS             5660..7522
                     /gene="dxs"
                     /locus_tag="OQN13_001084"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCS1614503.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            7577..8551
                     /gene="yajO"
                     /locus_tag="OQN13_001085"
     CDS             7577..8551
                     /gene="yajO"
                     /locus_tag="OQN13_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCS1614504.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(8605..9123)
                     /gene="pgpA"
                     /locus_tag="OQN13_001086"
     CDS             complement(8605..9123)
                     /gene="pgpA"
                     /locus_tag="OQN13_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCS1614505.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9101..10078)
                     /gene="thiL"
                     /locus_tag="OQN13_001087"
     CDS             complement(9101..10078)
                     /gene="thiL"
                     /locus_tag="OQN13_001087"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCS1614506.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(10156..10575)
                     /gene="nusB"
                     /locus_tag="OQN13_001088"
     CDS             complement(10156..10575)
                     /gene="nusB"
                     /locus_tag="OQN13_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCS1614507.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(10595..11065)
                     /gene="ribE"
                     /locus_tag="OQN13_001089"
     CDS             complement(10595..11065)
                     /gene="ribE"
                     /locus_tag="OQN13_001089"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCS1614508.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(11154..12257)
                     /gene="ribD"
                     /locus_tag="OQN13_001090"
     CDS             complement(11154..12257)
                     /gene="ribD"
                     /locus_tag="OQN13_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCS1614509.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGGVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(12261..12710)
                     /gene="nrdR"
                     /locus_tag="OQN13_001091"
     CDS             complement(12261..12710)
                     /gene="nrdR"
                     /locus_tag="OQN13_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCS1614510.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            12861..13400
                     /locus_tag="OQN13_001092"
     CDS             12861..13400
                     /locus_tag="OQN13_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCS1614511.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            13699..14583
                     /gene="tsx"
                     /locus_tag="OQN13_001093"
     CDS             13699..14583
                     /gene="tsx"
                     /locus_tag="OQN13_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCS1614512.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(14760..15107)
                     /locus_tag="OQN13_001094"
     CDS             complement(14760..15107)
                     /locus_tag="OQN13_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCS1614513.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(15236..16207)
                     /gene="secF"
                     /locus_tag="OQN13_001095"
     CDS             complement(15236..16207)
                     /gene="secF"
                     /locus_tag="OQN13_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCS1614514.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(16218..18065)
                     /gene="secD"
                     /locus_tag="OQN13_001096"
     CDS             complement(16218..18065)
                     /gene="secD"
                     /locus_tag="OQN13_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCS1614515.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18093..18425)
                     /gene="yajC"
                     /locus_tag="OQN13_001097"
     CDS             complement(18093..18425)
                     /gene="yajC"
                     /locus_tag="OQN13_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCS1614516.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(18448..19575)
                     /gene="tgt"
                     /locus_tag="OQN13_001098"
     CDS             complement(18448..19575)
                     /gene="tgt"
                     /locus_tag="OQN13_001098"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCS1614517.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(19631..20701)
                     /gene="queA"
                     /locus_tag="OQN13_001099"
     CDS             complement(19631..20701)
                     /gene="queA"
                     /locus_tag="OQN13_001099"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCS1614518.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            20794..21375
                     /gene="acpH"
                     /locus_tag="OQN13_001100"
     CDS             20794..21375
                     /gene="acpH"
                     /locus_tag="OQN13_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCS1614519.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(21380..23197)
                     /gene="malZ"
                     /locus_tag="OQN13_001101"
     CDS             complement(21380..23197)
                     /gene="malZ"
                     /locus_tag="OQN13_001101"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCS1614520.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(23353..24726)
                     /gene="proY"
                     /locus_tag="OQN13_001102"
     CDS             complement(23353..24726)
                     /gene="proY"
                     /locus_tag="OQN13_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCS1614521.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(24802..26121)
                     /gene="brnQ"
                     /locus_tag="OQN13_001103"
     CDS             complement(24802..26121)
                     /gene="brnQ"
                     /locus_tag="OQN13_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCS1614522.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(26528..27823)
                     /gene="phoR"
                     /locus_tag="OQN13_001104"
     CDS             complement(26528..27823)
                     /gene="phoR"
                     /locus_tag="OQN13_001104"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCS1614523.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(27881..28570)
                     /gene="phoB"
                     /locus_tag="OQN13_001105"
     CDS             complement(27881..28570)
                     /gene="phoB"
                     /locus_tag="OQN13_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCS1614524.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            28760..29962
                     /gene="sbcD"
                     /locus_tag="OQN13_001106"
     CDS             28760..29962
                     /gene="sbcD"
                     /locus_tag="OQN13_001106"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCS1614525.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETTQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            29959..33102
                     /gene="sbcC"
                     /locus_tag="OQN13_001107"
     CDS             29959..33102
                     /gene="sbcC"
                     /locus_tag="OQN13_001107"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCS1614526.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            33228..34412
                     /gene="araJ"
                     /locus_tag="OQN13_001108"
     CDS             33228..34412
                     /gene="araJ"
                     /locus_tag="OQN13_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCS1614527.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(34555..35463)
                     /gene="mak"
                     /locus_tag="OQN13_001109"
     CDS             complement(34555..35463)
                     /gene="mak"
                     /locus_tag="OQN13_001109"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCS1614528.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            35588..36499
                     /gene="rdgC"
                     /locus_tag="OQN13_001110"
     CDS             35588..36499
                     /gene="rdgC"
                     /locus_tag="OQN13_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCS1614529.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(36577..37032)
                     /locus_tag="OQN13_001111"
     CDS             complement(36577..37032)
                     /locus_tag="OQN13_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614530.1"
                     /translation="MTQHPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37029..37364)
                     /locus_tag="OQN13_001112"
                     /pseudo
     CDS             complement(37029..37364)
                     /locus_tag="OQN13_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9301 a        10091 c         9479 g         8578 t
ORIGIN      
        1 gaagcaagtc tttgtcttct gcgtcgggtt gatcgtggtt gcggaaattg tgttgtggaa
       61 cgcacaaacg cagttctggc aactggtggt cggcgtgcag cttttctttg tggcgtttaa
      121 tttgatggaa gcccttctgc cctcacttat cagtaaagag tcgccagcag gttacaaagg
      181 tacggcgatg ggtgtctact ccaccagcca gtttcttggc gtggcgattg gcggttcgct
      241 gggcggctgg attgacggca tgtttgacgg tcagggggta tttctcgctg gcgcaatgct
      301 ggccgcagtg tggctggcag tcgccagtac catgaaagaa ccgccgtatg tcagcagttt
      361 gcgcattgaa atcccggcgg acattgccgc aaacgaagcg ttaaaagtgc gtttgctgga
      421 aactgaaggc gtcaaagaag tgttgattgc agaagaagag cattcagctt atgtgaaaat
      481 cgacagcaaa gtgacaaatc gctttgaggt tgaacaggca attcgtcagg cataaaaaac
      541 gcggcgaatt gcctgctgcg ctacgcttac caggcctaca aagttcttga gttacaaacc
      601 gttgtaggcc gaatcaggcg ttcatgccgc atctgacatg aacaaaacgc acattgtcgc
      661 gattaatcgc ggaagttttt gaactggaac ggctggccga gatcgccacc acgtaccatc
      721 gccatgacag cctgcaaatc atcacgagat ttgccagtca cacggatctc atcgccctga
      781 atttgcgcct gcactttcag tttgctgtct ttaatcatct tgacgatttt cttctgggtc
      841 gcgctctcaa tgccctgttt cagtttcgct tccacaaacc aggttttacc gctatgaacg
      901 atattttccg gtacatccag tgaactgcct tcaatgccgc gcttcagcag cttggcacgc
      961 agaatatcca gcaactgatt gacctggaaa tcggactcgc tcaacacttt gatggttttg
     1021 ctggcgtcgt tcagctcaaa tgaggcttca acattacgga agtcaaaacg ggactccact
     1081 tcgcggctcg cgttatcgac cgcgttacgt gcttcctgaa gatcaacttc agagacaata
     1141 tcgaaagatg gcatcttttc ctctcccttc atttttgatg cgaagcataa tacccgcaaa
     1201 gttaaataac cgacagtcct gcaccaggac gtatccctta attgtgcata gtcacaacag
     1261 catgcacaat taagggatag ccttattgaa cacccggagt ggttgcgggt gaggaggaac
     1321 aatgaaaatt accgtattgg gatgcggtgc cttagggcaa ttatggctta cagcactttg
     1381 taaacagggt cacgaagttc agggctggct gcgcgtaccg caaccttatt gtagtgtgaa
     1441 tctggttgag actgatggtt cgatatttaa cgaatcgctg accgccaacg atcccgattt
     1501 tcttgccacc agcgatctgc tcctggtgac gctgaaagca tggcaggttt ccgatgccgt
     1561 caaaagcctc gcgtccacac tgcctgtaac cacgccaata ctgttaattc acaacggcat
     1621 gggcaccatc gaagagttgc aaaacattca gcagccatta ctgatgggca ccaccaccca
     1681 tgccgcccgc cgcgacggca atgtcattat tcatgtggca aacggaatca cgcatattgg
     1741 tccggcacgg caacaggacg gcgattacag ctatctggcg gatattttgc aaaccgtgtt
     1801 gcctgacgtt gcctggcata acaatattcg cgccgagctg tggcgcaagc tggccgtcaa
     1861 ctgtgtgatt aatccactga ccgccatctg gaattgcccg aatggtgaat tacgtcatca
     1921 tccgcaagaa attatgcaga tatgcgaaga agtcgcggca gtgatcgagc gcgaagggca
     1981 tcatacttca gcagaagatt tgcgtgatta cgtgatgcag gtgattgatg ccacagcgga
     2041 aaatatctcg tcgatgttgc aggatatccg cgcgctgcgc cacaccgaaa tcgactatat
     2101 caatggtttt ctcttacgcc gcgcccgcgc gcatgggatt gccgtaccgg aaaacacccg
     2161 cctgtttgaa atggtaaaaa gaaaggagag tgaatatgag cgcatcggca ctggtttgcc
     2221 tcgcccctgg tagtgaagag actgaagccg tcaccactat cgatctgctg gttcgcggcg
     2281 gtatcaaagt caccactgcc agcgtcgcca gcgatggcaa cctggccatt acctgctcgc
     2341 gcggcgtgaa gctgctggcg gatgcgccgc tggtcgaggt ggctgatggc gaatatgacg
     2401 tgatcgtact gcctggtggc attaaaggcg cagagtgttt tcgcgatagt accctgctgg
     2461 ttgaaaccgt taaacagttc caccgttccg ggcgtatcgt cgcggctatt tgcgccgcgc
     2521 cagccaccgt gctggtaccg cacgatatct tcccgattgg caatatgacc ggcttcccga
     2581 cgctgaaaga caaaattccc gccgaacaat ggcaggacaa gcgcgtcgtc tgggatgcac
     2641 gggtaaaatt gctgaccagc caggggccgg gtacggctat tgattttggt ctgaaaatta
     2701 tcgacctgct ggttgggcgc gagaaaaccc atgaagtggc atcacaactg gtgatggcgg
     2761 cagggattta taattattac gagtagtgtc ggatgcggca aacgtcgcat ctgaccagat
     2821 gcgacgtaaa aaccacaaat tatgggcgat acaccttcac attgttaaag ccctgctcgc
     2881 gcagatagag cgcctgcaga cggctcatca ccccgcgctc acaccacagt agccaggttc
     2941 tgttctggtc gagatcgcca aatttggtgc tcagtttata gaacggcaga gaaaccacgt
     3001 cgataccttc aaccttcagc ggcttatctt cctgctcatc gacagaacgg atatcgagga
     3061 tcacgtcgtt cgggccaaag ccattgacgg tttccacttc caccacttcc tgctcggtct
     3121 gctgggcgat ttcgcggata tcaacgttat tcgcttcctc aaccacttta tcgagaatac
     3181 tgaagtcgaa cttctcttct tccgcttcaa tcttcgattt aaccgctttc accgtcgggc
     3241 ttttggagat cacgccacaa tattctggca tcgtgcgagc aaagtcttca gtgccaatct
     3301 ggcgggccag gttgatgatg tgctctttgt cgtaagaaat cagcggacgc aggatcagcg
     3361 tatcagagac gttatcaatc aggcgcaggt tggtcagcgt ctgactggac acctggccga
     3421 gcgcttcgcc ggtgaccagc gcctgtacgc cgtaacgttc agccacttta gacgcggcac
     3481 gcaccatcat acgtttgagg ataacgccca tctgaccgtc gtcgattttc tcgagaattt
     3541 ccccgacgac cggttcgaaa ttaatggcga caaaacgcac gcggtgggag ctgccaaagc
     3601 ggttccacag ataatgcgcc acctgacgca cgccaatttc atgcgctgcg ccgccgaggt
     3661 taaagaagca gtaatgcacg cggcagccgc gacgcatcaa catataactg gaaacaccgg
     3721 agtcgaaacc accggaaatg agcgacagca catcttcctg ggtgccaatc gggaaaccgc
     3781 caataccttc gtagcggcct ttaatcagca gaagacgatc gtcttccact tccagatgga
     3841 cagttacatc cgggttagtc agcttcacgc gcgcggattc aatatgctga tttaaaccgc
     3901 cgccgacgta acgttccaca tcaatcgagc taaaatcatg tttgccacgg cgcttcacgc
     3961 gtacgcagaa ggttttgcct tccagctgat cgcgatattg aaccaacgct ttctcgaaaa
     4021 tatcgtgcat gtcggtaaac ggcacgtctt cgacttcgag aatatggtgg atacccggaa
     4081 tacgggtcag ggcgtcgcga atagccagac gctgtttttc atcttttgcg cgaacttcga
     4141 tgttatccca gtggcggacg acagcgagcg tctcatcata gtgctttaaa acgttacgaa
     4201 tgttcccggt aaggattttt ataaagcgca agcgcacaga ttggcttttg atggtgattt
     4261 ccgggaacaa tttaatgata aacttcatgg cggcaatggt tcgttggcaa gccttaagcg
     4321 acttgtatag ggaaaaatac agcagcccac acctgcggct gcatccaggc gcggaagtat
     4381 accactaaca tcgctttgct gcgcacatca ccttaccatt gcgcgttatt tgctatttgc
     4441 cctgagtccg ttaccatgac ggggcgaaaa atattgagag tcagacattc attatgccga
     4501 agaaaaatga ggcgcccgcc agctttgaaa aggcgctgag cgagctggaa cagattgtaa
     4561 cccgtctgga aagtggcgac ctgccgctgg aagaggcgct gaacgagttc gaacgcggcg
     4621 tgcagctggc acgtcagggg caggccaaat tacaacaagc cgaacagcgc gtacaaattc
     4681 tgctgtctga caatgaagac acctctctac ccccttttac accggacaat gagtaatgga
     4741 ctttccgcag caacttgaag cctgcgttaa gcaggccaac caggcgctga gccgttttat
     4801 cgccccactg ccctttcaga acactcccgt ggtcgaaacc atgcagtatg gcgcattatt
     4861 aggtggtaag cgcctgcgac cttttctggt ttatgccacc ggtcatatgt tcggcgttag
     4921 cacaaacacg ctggacgcac ccgctgccgc cgttgagtgt atccacgctt actcattaat
     4981 tcatgatgat ttaccggcga tggatgatga cgatctgcgt cgcggtttgc cgacctgcca
     5041 tgtgaagttt ggcgaagcaa acgcgattct cgctggcgac gctttacaaa cgctggcgtt
     5101 ctcgatttta agcgatgccg atatgccgga agtgtcggac cgcgacagaa tttcgatgat
     5161 ttcagaactg gcaagcgcca gcggtattgc cggaatgtgc ggtggtcagg cattagattt
     5221 agatgcggaa ggcaaacacg tacctctgga cgcactcgag cgtattcatc gtcataaaac
     5281 cggcgcattg attcgcgccg ccgttcgcct tggtgcatta agcgccggag ataaagggcg
     5341 tcgtgctctg ccagtactcg acaagtacgc agagagcatc ggccttgcct tccaggttca
     5401 ggatgacatc ctggatgtgg tgggagatac tgcaacgttg ggaaaacgcc agggtgccga
     5461 ccagcaactt ggtaaaagta cctaccctgc acttctgggt cttgagcaag cccggaagaa
     5521 agcccgggat ctgatcgacg atgcccgcca gtcgctgaaa caactggctg aacaatcact
     5581 cgatacctcg gcactggaag cgctagcgga ctacatcatc cagcgtaata aataaacaat
     5641 aagtattaat aggcccctga tgagttttga tattgccaaa tacccgaccc tggcactggt
     5701 cgactccacc caggagttac gactgttgcc gaaagagagt ttaccgaaac tctgcgacga
     5761 actgcgccgc tatttactcg acagcgtgag ccgttccagc gggcacttcg cctccgggct
     5821 gggcacggtc gaactgaccg tggcgctgca ctatgtctac aacaccccgt ttgaccaatt
     5881 gatttgggat gtggggcatc aggcttatcc gcataaaatt ttgaccggac gccgcgacaa
     5941 aatcggcacc atccgtcaga aaggcggtct gcatccgttc ccgtggcgcg gcgaaagcga
     6001 atatgacgta ttaagcgtcg ggcattcatc aacctccatc agtgccggaa ttggtattgc
     6061 ggttactgcc gagaaagaag gcaaaaatcg ccgcaccgtc tgtgtcattg gcgatggcgc
     6121 gattaccgct ggcatggcgt ttgaagcgat gaatcacgcg ggcgatatcc gtcctgatat
     6181 gctggtggtc ctcaacgaca atgaaatgtc gatttccgaa aatgtcggcg cgctcaataa
     6241 ccatctggca cagctgcttt ccggtaagct ttactcttcg ctgcgcgaag gcgggaaaaa
     6301 agttttctct ggcgttccgc caattaaaga gctgctcaaa cgtaccgaag aacatattaa
     6361 aggcatggta gtgcctggca cgttgtttga agagctgggc tttaactaca tcggcccggt
     6421 tgacggtcac gatgtgctgg ggcttatcac cacgctgaag aacatgcgcg acctgaaagg
     6481 cccgcagttc ctgcatatca tgaccaaaaa aggtcgtggt tatgaaccgg cagaaaaaga
     6541 ccccatcact ttccacgccg tgcctaaatt tgatccctcc agcggttgtt tgccgaaaag
     6601 tagcggcggt ttgccgagct attcaaaaat ctttggcgac tggttgtgcg aaacggcagc
     6661 gaaagacaac aagctgatgg cgattactcc ggcgatgcgt gaaggttccg gcatggtcga
     6721 gttttcacgt aaattcccgg atcgttactt cgacgtggca atcgccgagc aacacgcggt
     6781 gacctttgcc gccggtctgg cgattggtgg gtacaaaccc attgtcgcga tttactccac
     6841 tttcctgcaa cgcgcctatg atcaggtgct gcatgacgtg gcgattcaaa agctcccggt
     6901 cctgttcgcc atcgaccgcg cgggcattgt tggtgctgac ggtcaaaccc atcagggcgc
     6961 ttttgacctc tcttacctgc gctgcatacc ggaaatggtc attatgaccc cgagcgatga
     7021 aaacgaatgt cgccagatgc tctataccgg ctatcactat aacgacggcc cgtccgcggt
     7081 gcgctacccg cgcggtaacg cggttggcgt ggaactgacg ccgctggaaa aactgccaat
     7141 tggcaaaggc attgtgaagc gtcgtggcga gaaactggcg atccttaact ttggtacgct
     7201 gatgccagac gcggcgaaag tcgctgaatc gctgaacgct acgctggtcg atatgcgttt
     7261 tgtgaaaccg cttgatgaag cgttaattct ggaaatggcc gccagccatg aagcgctggt
     7321 caccgtagaa gaaaacgcca ttatgggcgg cgcaggcagc ggcgtgaacg aagtgctaat
     7381 ggcccatcgt aaaccagtac ccgtgctgaa cattggcctg cctgacttct ttattccaca
     7441 aggaactcag gaagaaatgc gcgccgaact cggcctcgat gccgccggta tggaagccaa
     7501 aatcaaggcc tggctggcat aatccctact ccactcctgc tatgcttaag aaattattca
     7561 tggcaggagt ggcagcatgc aatacaaccc cttaggaaaa accgaccttc gcgtttcccg
     7621 actttgcctc ggctgtatga cctttggcga gccagatcgt ggtaatcacg catggacact
     7681 accggaagag agtagccgtc ccatcattaa acgcgcgctg gaaggcggca taaatttctt
     7741 tgataccgcc aatagctatt ccgatggcag cagcgaagag atcgttggtc gcgcactgcg
     7801 ggatttcgcc cgtcgtgaag acgtggtcgt tgcgacgaaa gtgttccatc gcgttggtga
     7861 tttaccggaa ggattatccc gtgcacaaat tttgcgctct atcaacgaca gcctgcgccg
     7921 tctcggcatg gattatgtcg atatcctgca aattcatcgc tgggattaca acacgccgat
     7981 cgaagagacg ctggaagccc tgaacgacgt ggtaaaagcc gggaaagcgc gttatatcgg
     8041 cgcgtcatcc atgcacgctt cgcagtttgc tcaggccctg aagctacaaa aacagcacgg
     8101 ctgggcgcag tttatcagta tgcaggatca ctacaatctg atttatcgcg aagaagagcg
     8161 cgagatgctg cctctgtgtt atcaggaggg cgtggcggtg attccgtgga gcccgctggc
     8221 gcgggggcga ctgacgcgtc cgtggggaga aactaccgca cgactggtgt ctgatgaggt
     8281 ggggaaaaat ctctataaag aaagcgatga aaatgacgcg cagatcgcag agcggttaac
     8341 gggcgtcagt gaagaactcg gtgcaacacg agcacaagtt gcgctggcct ggttattgag
     8401 taaaccgggc attgccgcac cgattatcgg tacatcgcgg gaagaacagc ttgatgagct
     8461 gctgaacgcg gtggatatca ctttgaagcc agaacagatt gccgaactgg aaacgccgta
     8521 taaaccgcat gcggtcgtag gatttaaata agagtccagg cctgatgaga cgtgacaagc
     8581 gtcacatcag gcatcggtgc acaactacga cagaataccc agcggccagt gatgaccgat
     8641 aaagtacagg atgcctgcgg aaatcacccc ggcgacaata tcgtcgatca tgatccccat
     8701 gccgccatgt acattgcgat caaaccagcg gatcggccac ggcttccaca tatccagaat
     8761 acggaaaatc acaaacccgg cggcaaccca ctgccagtca ttggtcggca gcgccatgag
     8821 cgtgatccac ataccaataa attcgtccca gacaatgctg ccgtgatcgt gcacgcccat
     8881 atctttcgcc gtttgatgac aaagatagac gccgatacag atccccaaca tcaccaccag
     8941 cgagtagagc tgccagggta aaaaggtcat cagataccag aacggaatcg ctgccagcga
     9001 tcccatcgtg ccaggaacga ttgggcttaa tccactcccg aatccgacag caagcagatg
     9061 ccacggatta ctcatcttca ggcgactttt cgcgacatct ttatggcgtg gcaaaatggt
     9121 catatccttt ccagtcaaac gtgacaggtt cgccgtcacg aataaaacaa agcccttcga
     9181 tatcggcggt catttgcccg atacaggtaa acggtacgcc aaggtgtccg agagcaacat
     9241 ccagcgcgcc acggttcagt tccggcaccg tgaaacacaa ctcgtaatct tcaccgccag
     9301 agagcgccca gcgcagcgcc tgttccggtt caacatggcg agaaagcgca tcagaaaacg
     9361 gcagcaatgc caggtcaata cgtgcgccgc agtcgctggc tttcacgata tgcccgagat
     9421 cggaaatcag accgtcagag agatcgatag ctgaatttgc cagatcgcgc agtgcctgcc
     9481 cctgtaaaat acgcggcgat ggacggagat gacgtttgat caagtagtca gcatctttag
     9541 catcggcaac ctgcaaacgg ttttgcaaaa tcgccagccc ggcggcgcta tcgcccggtg
     9601 taccggtcac atagatccag tcacccggtt tcgccccaga gcgcgttaag gctcgtccca
     9661 tcggaacaaa gccgtggata cccaacgtca ttgataatgg cccacgcgtg gtatcgccgc
     9721 caatgagttg catatcgtaa taattgagaa gatcaaacaa actgtcgctg aaggactcaa
     9781 gccacgcttc gtctacgtcc ggtaaggtta atgccagcgt cagccaggcc ggatcggccc
     9841 ccatcgctgc cagatcgctt aggttcaccg ccagtgcttt ataagccaga tcagcaggat
     9901 cgatatcagg gaggaaatgg ttacccgcca ccagcgtatc agtgctgatc gccagggtct
     9961 gtttctcggg gatattgaga agtgcgcaat cgtcgccgat gcccagttcg acatcaagac
    10021 gagaacttct tacacggtca aaataacggg caatcaggga gaactcgcca catgccatac
    10081 gttatgcctc agcaggtaaa aaagaaaagg ccgacgatca tggaacggtc ttccgtgaat
    10141 ctaccggcct ggatatcact ttttgttagg gcgaatcaca ggtgctgctt tatcgagtac
    10201 gccgttgacg aacttatggc tgtcttctgc gccgaacgat ttcgccagtt cgatcgcttc
    10261 gttaatggcc actttgtatg gcacatcgct acgtttagac agttcgtaca gcgcaatgcg
    10321 cagtactgct ttttctacct gtcccagttc ttccagcagg cgggacaggt atggcttcat
    10381 cagtccgtcg aggtatgcgg tattagtcgc caccccggcc agcagctcac ggaagtacag
    10441 gacgtcaacg tcttttacat cctgttcagc caggaactgg tattcaacat cagcgatgtc
    10501 gttctgggac aactgccagg agtagagcgc ctggacggca cactcacgag cgcggcgacg
    10561 agcagcaggt ttcacggatt tccccttact aatttcaggc cttgatggct ttcaatacat
    10621 taatcatttc aagcgcggtc agtgcagctt ctgcaccttt gttgccagct ttggtgccag
    10681 cacgttcgat cgcttgttca atgctttcag tggtcagaac cccaaaagca accggaattt
    10741 cgctgtcctg ggcaacatgc gccaggccgt tgcttgcacc accagcgaca tattcaaagt
    10801 gggcagtgcc accacgaata accgtaccca gcgcaatcac cgcgtcgtat ttaccggttt
    10861 tagccagtgc acccgccgcc agcggcagct cataggcacc aggcacccaa acaacggtaa
    10921 tgttttcatc ttttacctga ccgatacgtt tcagtgcgtc aattgcacct tccagcaggc
    10981 tgtcattgat aaagttgttg aaacgcgcga tggtgatggc gacgcgagcg tccggggtag
    11041 caacgttagc ttcaataatg ttcattttct tccttcgggt tcgagtatgg ccccgcaggg
    11101 gggcggattt tagcataata tttcgtgcgc tgcttccctt tcgagccggg agatcatgca
    11161 cccactaaat gcaggcaaac atccgggcct acatgacgta tctctttgaa tttaaattgg
    11221 ggggcgtcgg ctaatttctc aagccctggc agcgtgcata atccgcgggc gtcgctgcct
    11281 aatagtttag gtgcgatata gacaatcagc tcatcgacta aacccgcctg cagcaatgcg
    11341 ccagcgagcg ttggccccgc ttccacccag atgctgttaa tttgctgttt acccagttgc
    11401 atcatcagta caaccagatc cagatgacct ttatgctctg gaatcagcaa ggtacgcacc
    11461 gtttccggcc actcactaga atcttcctgc gtacgcgcga accaggtttc gccgggctgc
    11521 tgcacaatgc gatgttccgg cgtcacgcga ttttggctat caatcacaat acgtactggc
    11581 tggcggagat tttgttgcgg gtagagcgcc tgagtttgtt catccagttc agaccaacgc
    11641 actgttaagg ctggatcatc cgccagcacc gtggcgctgc tggttaaaat ggcatgactt
    11701 tgtgcgcgct gtcgttgtac gtcgcgccgc gcctgaggcg aagtgatcca ctggctttcg
    11761 ccgctcgcca tcgccgtgcg gccatcaagc gatgcgccaa gttttaactg aatataagga
    11821 aagccggtgc gcatccgctt gagaaagcct ttattcaatt gctcggcttc actcatcatc
    11881 agcccgtggc tgacgtcaat gccagcctgt tgcagacggt aaagtccacg cccagcgacc
    11941 tgcgggttag gatcctgcat cgctgcgacc acgcgcgcta cgccagcggc gatgagtgcg
    12001 tcacagcacg gtggcgtacg gccatgatgg ctacagggtt cgagtgtcac atacgccgtt
    12061 gcgcctttgg ctttttcacc cgccatacgc aacgcgtgta cttcggcatg tggttcaccc
    12121 gctcgttggt ggtaaccttc accgacaatt tcgccatctt tgacaatgac gcccccgaca
    12181 ttcgggttgg ggtgggtggt aaaacgtcct cgttgcgcca gctttagcgc ccgcgccatg
    12241 taatactcgt cctgcacggc ttagtcctcc aggcgcgcga tctcttcgcc aaattctttg
    12301 atatcttcga aactgcggta gacagaggca aaacggatat aggcgacttt atcgagcttt
    12361 ttcaattgct ccatcaccag attgccaatc atcttgctcg gtacttcgcg ctcaccggtg
    12421 gcgcgcagct gcgatttaat atgattgatt gccatttcaa cgtcatcgga actcaccgga
    12481 cgtttttcca gcgcccgcag cattccgcta cgcaatttct cttcattaaa cggttcacgc
    12541 acgtcgttgc tttttacaac acgcggcata accagctccg ccacttcaaa ggtggtgaaa
    12601 cgttcattac acaccagaca ctgccgacgg cggcgtacgg atgaaccctc gcccacgaga
    12661 cgagagtcaa ttaccttagt gtccacggcg aaacagaatg ggcaatgcat acggcgtcct
    12721 gaccaggtgg ttaacggaaa tctattttac cctgaactga cgatacaaca aaggcgcaac
    12781 gcttttgaga taaaggatcg atgctttcgc tgcttttacc ctctaccatt aggctaattg
    12841 cgacgagaaa gggacgacct atgaatacta acgtttttcg actgctcctg ctgggaagcc
    12901 tgttcagcct tagcgcctgt gtgcagcaaa gtgaagtgcg acagatgaaa cacagcgtca
    12961 gcacgctgaa ccaggagatg acgcaactca atcaagaaac agtcaaaatc actcagcaaa
    13021 acaggctgaa tgcaaaatcc agcagtgggg tttaccttct gcctggagcg aaaacaccgg
    13081 caagactgga aagccagatc ggtactttac gtatgtcgct ggtgaatatt acgcctgata
    13141 cagatggcac cacccttaca ctacgtattc agggtgagtc gaatgatccc ttgcccgcat
    13201 tcagcggaac tgttgaatat gggcaaattc agggaacaat agacaacttt caggaaatca
    13261 atgtgcagaa tcaattgatt aatgcccctg ccagcgtcct cgcccccagc gatgttgata
    13321 ttccgttaca attaaagggg atttctgtgg atcagttagg ctttgtacgt atccacgaca
    13381 ttcaacctgt tatgcagtaa acgtatttcg ggacgatttt gtgcgtcccg caacatcttt
    13441 ccccgtcatt ttgttactct gcttacatca cctggattga tagtaaaagt ttgcaacaag
    13501 ggcgaaagtc agtacaatcc ccgcccgaat gtgtgtaaac gtgaacgcaa tcgattacgt
    13561 aaatgataga actgtgaaac gaaacatatt tttgtgagca atgattttta taataggctc
    13621 ctctgtatac gaaatattta gaaacgcaat ttgcgccttt ttcactcccg caagggattt
    13681 tcaaacagtg gcatacatat gaaaaaaaca ttactggcag ccggtgcggt actggcgctc
    13741 tcttcgtctt ttactgtcaa cgcagctgaa aacgacaaac cgcagtatct ttccgactgg
    13801 tggcaccaga gcgttaacgt tgtcggaagc tatcacaccc gtttcggacc gcagatccgc
    13861 aacgatacct accttgagta cgaagcattc gctaaaaaag actggttcga cttctatggt
    13921 tatgcggatg cgccggtatt cttcggcggt aactccgatg ctaaaggtat ctggaaccac
    13981 ggttctccgc tgtttatgga aatcgaacca cgtttctcca tcgacaagct gaccaatact
    14041 gaccttagct tcggtccgtt caaagagtgg tacttcgcga acaactacat ttacgacatg
    14101 ggtcgtaata aagatggtcg ccagagcacc tggtacatgg gtctgggtac cgacatcgat
    14161 actggtctgc cgatgagcct gtccatgaac gtctatgcga aataccagtg gcagaactat
    14221 gacgcagcga acgaaaacga gtgggacggt taccgtttca aaattaaata ctttgtgccg
    14281 attaccgatc tgtggggcgg tcagctgagc tacatcggct tcaccaactt cgactggggt
    14341 tccgatttag gggatgacag cggtaacgca atcaacggta ttaagacccg tactaataac
    14401 tctatcgctt ccagccatat tctggctctg aactacgatc actggcacta ctctgtcgta
    14461 gctcgttact ggcacgacgg tggtcagtgg aacgacgatg cagaactgaa cttcggcaac
    14521 ggcaacttca acgttcgctc taccggctgg ggtggttacc tggtagtagg ttacaacttc
    14581 tgattatgaa aatgccggga tttattcccg gcatttctga ttgttaacaa cgtgtgcttt
    14641 gttcatgccg gatgcggcgt aaacgctttg tccggcccac aaaagcacgt aaattcaata
    14701 tattgctgaa ataacgtagg cctgataagc gtagcgcatc aggcaatttt acgttttaat
    14761 cacttcttct tgttcatcat cgctttcagg tcagcgaatg ggttgtactt cgcttcaccg
    14821 acatctttct gcgcatcttc ccctgcgata acggtcgtac catactgatc cgcttcggta
    14881 tatttcgaat gctcatgatc gtggcaatag agacaaagca attcccagtt actgccatct
    14941 tccgggttat tggtatggtc gtgatcaatg tggtgaacgg taagttcacg caggttggaa
    15001 taaacaaact cgcgggaaca gcgaccgcag acccacggat agatttttaa tgctttttcg
    15061 cgatagccgc tttctaaccg cgcatagttt tttgggatga tagccatgaa aagttacctg
    15121 gatgaaatgt agggttacgc gcagtttccc aagatgcggg gattttcgca atctccatac
    15181 atcaaaaaaa atcccgatct tctgaccggg attttcaaca tcaacgggaa cttgattacg
    15241 gcagaattga cggctgatcc gccccttctt tttccacttt ctgctgcaac atgtgttcgc
    15301 gcttcatacc cagtttcaga gccaacgcag atgctacgta gatggaagat gcagtgccga
    15361 tggaaacacc gataagcatg gtcagcgaga agccttccag taccggacca ccgaagagat
    15421 acagcatcag gataaccatc aaggtagtac cggatgtgat caaggtacgg tgcagcgtct
    15481 gggtcaagga cacgttaaag atttcgtaag gcgtaccgcg acggatcttg cggaagtttt
    15541 cacgaattcg gtccgatacc acgatactgt cgttaagcga gtaaccgata accgacatca
    15601 acgatgccac aatggtcagg tcaatctcga tatggaataa cgacaaaata cccagcgtaa
    15661 tgataacgtc gtgcgccagc gcaataacca cccctgccgc cagtcgccac tcaaagcgga
    15721 aacctacgta cacgaggata gacagcagcg ctgccatcaa cgccatcgca ccggtttgcg
    15781 caaggtctgc ccccacgctc ggaccgacga actcaatacg cttcactgct gcattctgat
    15841 tggtggattc gttaatcacc ttcagaacct ggctgcccag cacctgaccg ccggtttcgc
    15901 cttcagcagg cggcatacgg accatgatgt cgtggctgct accaaagttt tgcagcattg
    15961 gctcttcaaa accggctttt tgcaatgcat cacgcattac gtcaatttca gccggttttt
    16021 cgagcgtaat ttcgataacc gtaccaccgg tgaaatccag cccccagtta aagccgcgca
    16081 cgcccataat aacgatagca gcgattaaca gcagaccaga gatgccgaaa gcccagtagt
    16141 cccagcgcat aaagtcatag actttacggc cgtggtttag ttgttcaaca gtatattcct
    16201 gtgccacatc gcactcctca gattgacagc ttcttgacgc gcttgccgcc atatagcagg
    16261 tttacgatgg cacgcgtacc gacaatcgcg gtaaacatcg acgtcgccac accgataccg
    16321 gtagtaatcg cgaacccttt aattgccccg gtacccactg cgtacaggat gatgacttta
    16381 atcagcgtgg tgatgttcgc atcgaagata gaactgaatg cgccacgata accttcatca
    16441 attgcctgtt gaacagtacg tccgttgctc aactcttctt taatacgttc gttgatcagt
    16501 acgttcgcat cgaccgctac cgcaagggtt aagacgatac ccgcgatacc tggcatactc
    16561 agtgttgcgc ctggcaacag cgacataata ccgacgatca agatcaagtt ggcaatcaga
    16621 gcactggtcg caatcagacc aaacttctta tagaagatga tcatgaacag aatagaaacc
    16681 agcagaccgg caaggcaagc ctccagcccc tgctcaatgt tctgcatacc cagggtcggg
    16741 ccaattgtac gttcttcaac aatctgaatc ggcgcgatca acgcaccggc acgcagcagc
    16801 agtgacagct gacgggcttc gttcgggttg ttgatgccgg tgatacggaa gctgttaccc
    16861 agacgagact ggatgttggc gatgttaatc acctcttcct gtttcaccag aaccgcacga
    16921 ccatttgcat ctttcttacc gctgtctttg tactccacaa acagggttgc catcggtttg
    16981 ccgatgttgt ccttggtgaa gttagacata atgttgccac cagcgctatc gagcgagatg
    17041 ttaacctgcg gctggttgta ttcgtcctgg ctggaagtgg agtcggtgat atggtcaccg
    17101 gtcagaatta cgcgtttgta cagcacaacc ggctgacctt cgcgggtctg tttcacttca
    17161 gagtcgcccg gtacgcgacc ggatgccgca gcggcctgat caacattggt gtttaccaga
    17221 cggaattcca gcgttgccgt cgcgcccaga atctctttcg cacgcgcagt gtcctgaata
    17281 ccaggcagtt caacaacgat acggtcagca ccctgacgct gaaccaccgg ctccgccacg
    17341 ccaagttggt ttacacggtt acgcaggata ttaatgttct gctgcaccgc atattcacgc
    17401 gcttcactca gacgagcatc gctcattacc gcacgcagct ggttgctgcc ctggctgcta
    17461 atcaccaggt ccggatggcg cttgctcaga tacgcaatgg cttcatcacg agctttagca
    17521 tcgcggaaag tgatgctcag gccgtagttg ttttctttac gaacagtggt atacgggatg
    17581 cccttttcgc gcaggtcact gcgtaggcta tcgatatttt gttcctggag tttgccaagc
    17641 gcggtgtcca tatccacttc catcaggaag tgaacgccgc cacgcaggtc aaggccgagc
    17701 ttcatcggct cagcatgaat agctgccagc cagcgcggcg ttgccggggc aaggttaagc
    17761 gccacgacgt atttgtcacc cataacgccc attaatgctt cacgagcgcg caactgggtg
    17821 tcagtggagt cgaagcgcgc aagaatagcg ccctcttcca gtgccacaga cttagcagtt
    17881 attttttctt cttgtaacgt tttctggacc tggatcagcg tttgctcact ggcggcgact
    17941 ccgcgcgcac cagtgatctg aacagccgga tcctcaccaa acaggttggg aagcgcatac
    18001 agcagaccga tgacaatcac cacgatcagc atgacgtact tccacaaagg ataacggttt
    18061 aacacggcaa ttcccttagg gaaaaatttt aattacagcg ccttcatggt gcctttcggc
    18121 aggacggcag ctacgaagtc acgtttaata actacttcag tggtgtcatt cagcgcgata
    18181 gcaatgtagc cgttttccgc tactttggtt acgcgaccaa ccaggccacc gttcgtcaga
    18241 acttcatcac ctttggcaat ggagtccatc agctttttgt gttctttggt gcgcttctgc
    18301 tgtggacgca ggatcatgaa atagaaaatc agaccgaata ccaccagcat caaaatcaaa
    18361 gacatcgggc taccttgcgc cggtgcaccc gttgccgcta ccgcatcaga aataaaaaag
    18421 ctcattaaat ttccctcatt attaatatta atcaacgttc aaaggtggta cttctcgccc
    18481 ctgacgctgg taaaaatcag ttacgaagct ctctaattta ccctcttcaa tagccttgcg
    18541 taaacccgcc atcaaacgct ggtagtaacg aaggttatga atggtgttga gtcgcgcgcc
    18601 taatatttcg ttgcaacggt caagatgatg caagtaagcg cgtgaataat tgcgacaggt
    18661 gtagcaatca cactcaggat caagtgggcc agtatcgctc ttatacttcg cattgcggat
    18721 tttcaccacg ccatcggtca cgaacaaatg accatttcgg gcgttgcggg ttggcattac
    18781 gcagtcaaac atatcgatac cacgacgtac gccttcaacc aggtcttctg gtttaccaac
    18841 gcccatcagg taacgcggtt tgtctgccgg aatttgcggg catacatgct ccagaatgcg
    18901 gtgcatatct gctttcggct cacccacagc cagaccgccg acagcgtagc catcaaaacc
    18961 gatatctacc agacctttaa cagaaatatc acgtaaatct tcgtaaacgc tgccctgaat
    19021 gataccaaac agcgcatttt tgtttccgag actgtcaaaa cgctcacggc tacgcttcgc
    19081 ccaacgcaga gacatctcca tggagcgttt tgcgtaatcc cagtcagcag gatacggcgt
    19141 acactcatca aagatcatga cgatatccga accaagatcg tactgaatct ccattgattt
    19201 ttcaggatcg aggaaaatcg gatcgccgtt gatcgggtta cggaagtgca cgccctgttc
    19261 ggtgatttta cgaatatcgc caaggctgaa gacctggaag ccgccggagt cggtgaggat
    19321 cggccccttc cactgcataa aatcgtgcag atcgccgtgc agtttcatga tttcctggcc
    19381 cgggcgcagc cacaggtgga aggtgttgcc gaggataatt tgcgcgccag tggcttcaac
    19441 ttcttccggc gtcatccctt ttacggtgcc gtaggtgcca acaggcataa aacaaggcgt
    19501 ttccactacg ccacgatcaa agaccaggcg gccacggcgt gcgcgaccgt cggtggtgtc
    19561 cagttcaaat ttcatttttt ctccactacg tcagaaaaac agtccaacgt tttaaaccag
    19621 cgccacggaa ttactccccg acgcgctcat taattgcctg cggattgtac gtgataaaca
    19681 tcgcatcacc gtaactaaaa aagcgatatt tctcttctac cgctgcttta taggcgttca
    19741 tggtgtgttg ataaccggca aaggccgaaa ccagcataat cagcgtcgat tccggcaggt
    19801 ggaagtttgt caccagcgca tcgaccactt tgtactggaa gcccggatag ataaagattt
    19861 gggtatcgtc aaagaacggt tcgataagat cgtttttcgc cgcctgagcc gcgctttcca
    19921 gcgaacgtac tgaggtggtg ccaaccgcaa tcacccggtt accgcgcgct ttcgccgcca
    19981 gtaccgcgtc taccacatcc tgcggcactt cagcgtattc cgagtgcatg atgtgatctt
    20041 caatggtgtc gacgcgcacc ggctggaagg tgcccgcacc aacgtgcaac gtcacaaacg
    20101 ccatctccac gcctttggcg cgtaattttt ccagcaaagg ttcgtcaaaa tgcagacctg
    20161 cggtcggggc tgcaaccgcg cccggttttt cgctataaac ggtttgataa agttcgcggt
    20221 cagcgtcttc gtccggacgg tcgatatacg gcggcagcgg catatggccg atgctgttga
    20281 gaatatccag caccgagcgt tcatcattaa attcgacttc aaacagtgcg ccgtggcgcg
    20341 cggtcattgt tgcgttaata ctttcgtcat cgcccagcag cagttctgcg ccaggttttg
    20401 gcgctttcga ggcgcgaata tgcgcaagaa tgcgtttgtc gtcgagcatc cgttcaacca
    20461 gcacttcaat cttgccgccg ctggctttac gcccaaacag gcgcgccggg atcacgcggg
    20521 tattattaaa aaccagaaga tcgccggggt tgagcttatc aagtaaatcg gtgaaagtac
    20581 cgtgcgtcag cgcgcccgtc ggcccgtcca gcgacagtaa acgacagcta ctgcgttcag
    20641 gcatgggata gtgggcaatc agggattcgg gcaattcaaa ggagaaatcg gtaacgcgca
    20701 tgacactgac tcagactaaa ataagaggcg ggtagtctag tgccggggcg ctctccctgc
    20761 aacctttacc cccttttgga tagaatataa tcgatgaatt ttttagctca cctgcattta
    20821 gcccatctcg cggaaagctc gctttccggc aatttactgg ctgatttcgt acgcggaaat
    20881 cctgaagaaa gttttccgcc cgacgtcgtg gctggcattc atatgcatcg acgtatcgac
    20941 gtattaaccg acaatctgcc ggaagtccgc gaagcacggg aatggtttcg tagtgaaact
    21001 cgccgcgttg cgcctattac gctggatgtc atgtgggatc actttctttc ccgccactgg
    21061 tcgcagctgt cgccggactt tccgctacag gaatttgtct gttatgcccg cgagcaagtg
    21121 atgacgattt tgccggactc accgccacgt tttatcaatc tgaacaatta cttgtggtca
    21181 gagcagtggc tggtgcgcta tcgcgatatg gatttcatcc agaacgtgtt aaacggcatg
    21241 gcaagccgcc gcccacgtct ggatgccctg cgtgactcct ggtacgattt agacgctcat
    21301 tatgacgccc tcgaaacccg cttctggcag ttttatccgc ggatgatggc gcaggcgtca
    21361 cacaaggcgt tataaaacgt tagttcatcc ataccgtagc cgaaatggca ggcaaagcca
    21421 gaatcccgtc agtcagttgc ccatgccttt ctttgcattg ccattgcacg gcattgagca
    21481 acggtgacgc gggtagcacg acttcacaag cttcgccacg gttgattgcc accagtacac
    21541 gctgctgatt cagcacgcgg acaaatacca ccacgttatc ttccgcatac agcacctgac
    21601 agccgccatg acgtagcgcc tgacttttct tacgcagcgc aatcattcgc tggtacagcg
    21661 cgaataacgc cgtatcctgc ttttccacct gccaggggaa cggtttacgg caaaacggat
    21721 cgtttttgcc atccagtcct acttcatcac cgtaataaat gcacggtaca ccaggccagg
    21781 tgaacagcca gaccaccgcc agcggcaggc gcgcaatatc ccgaccgagc agcgttttaa
    21841 atcgcgcagt atcgtggctg tcgagctgat taaacatacg taattgttgt tgatgagaaa
    21901 gccctgcgcg gtaattatcc atccaggcca tacaggtttg ggcatcaatt tgctgcggat
    21961 cgtaagagat atcggtattg gcaagaaatc cccacaacgg gaatgtgaag ccacgatagt
    22021 tcatggcggc atcttccaca tcggcctgta accattgccg tgcatcgcca aaatgttcgc
    22081 cgacaatata cgcttccggc tgggtttctt tcgccgcttc ggtgctccca gcaacgtgct
    22141 gcatattatt gcgcgcccca cccgcctccc ctagcatatg caccacatcc agccgccagc
    22201 cgtccatact ccacggcgct ttcagccaat ggcggacaat actgtcttcc ccgcgataaa
    22261 tttcattcac cagactttcc gactgataat ccagcttcgg caagctggca tagccaagcc
    22321 agtcgagcgc cgtgccatca tcactaaacg agtaccagtc gcgccagggc gattcagggt
    22381 tgtgacaagc tccccccgtg ccacgattgt gcctatcaaa ccaggcatgg gaatcgccac
    22441 tgtggttaaa cacgccatcc agccccagcc gcattcccag ctgctgcgta ttgtggcgta
    22501 agcgcagcaa cgccccatca ccgccaaact gcggatcgac atggcgataa tcctcggtat
    22561 cgtatttatg tacgctggga gctttaaaca ccggattgag atacagcgcc gtcacgccaa
    22621 gctttttcag atacggcagt ttttcgctta tcccgtccag atcgccgcca tagaacgttg
    22681 atccgcccgc ctgcgccgtg accggttcat cccagtcacg caagatgatc tcttgtccgg
    22741 ctgcatgatg gtaatagaca tgatcctgtt cagcttcacg aggaagacta cgcgcaaaac
    22801 gatcagggaa gatctgataa aaaatctgat ccgcagccca ttgtgggccg atatccggta
    22861 catcgacggc aaactgctcc agtcgtgccg gcggcattcg gctgaagccc tgcggtgtaa
    22921 accaacgctg gcgatcgtgc cacagcagtt tgaaactgta acgccgccgg ggttgtccgc
    22981 tggagagatc aatcgccgca cgccatgcgg tgacgccagg ctgctgctga ctgcgatgct
    23041 tatgcatcga tactgacatt tcttcgttat cgtgttctgt acgcagcata atgcgctgcg
    23101 gtgggtcttc gcccgtcagc cacagtgtaa tgagcagttg atctttgctt tgtttaacaa
    23161 atgggggcac cggcaggtgc catgcattta acatcataat tcccctgtga tgaaaatctg
    23221 atcaccttgc catagcctaa tgcaggatca ctcatcatct gaaggatatt tgggggagga
    23281 ggacgggggc ggataacttt cggaatagcc ggatcaggat ttcctcctga accggcttac
    23341 agaacgcaag gattactggt tttcagccag ctgacgatcg tggcgacgct taaacatcca
    23401 gccaatcaac agcacaacaa tccacgcgaa accgacatac agcgagatac gcgtatccgg
    23461 gtgataacca atcaacccga taataaagag caggaaaatc agcccgccga tggtcgttgc
    23521 taccccaccc ggcactttaa atttcagcgc cttaacttct tctggcggca aacagcgacg
    23581 gaaggcaatt tgcgacagca ggatcataat ccacacccac accgtggcga aggttgccag
    23641 cgaagcgatc accaggaaga cgttttccgg catgatgtag ttcagataca ccgcaaacag
    23701 cagcgcggta gtcatcacca gcaccgtaac ccacggaata ccgcgacgcg acgttttgct
    23761 gaaaattttc ggcgcgctgc cctgctctgc cataccgtgg agcatacggc ctacgccaaa
    23821 gacgtcactg ttaattgccg atagcgaagc ggtcagcaca acaaagttaa gaatgctagc
    23881 ggcaaaggta atgcccatat gctggaacgt cagcacgaac gggctaccgg cagtgccaac
    23941 ctgattccac gggtagatag acataatgac gaacagcgta ccgacgtaga acaccagaat
    24001 acgcatcggc acggagttaa tcgcacgcgg tatcgatttc tcaggatctt tcgcttcacc
    24061 ggcggtaatc ccgataattt cgatcccacc gtaagcaaac atcaccattt gcaacgacat
    24121 caccatccca agccagccgt tactgaagaa gccgccgttg ctccacagat tatgaatacc
    24181 ggtcggttgc ccgccgttgc caatccccca gatgatgatg ccgaaaccag cgacaatcat
    24241 gatgatgatg gtggcgactt taaagaacga gaaccagaat tccagctccc cgaagacctt
    24301 cacgctcatc aggtttacgg cgcaaatgat cagcaccacg ctcagtaccc aaatccagtg
    24361 cggcaccgtc gggaaccaga cacccatata gataccaaaa gcggtcacat cggcgatggc
    24421 gacaataagg atttcaaagc agtaggtcca gccggtaatg taacctgcca gcgggccgag
    24481 gttttcctgc gcataacgcg agaaagagct ggcggccggg ttatgtaccg acatttcccc
    24541 cagcgcacgc ataatgatat acgccgcgat accaccgata atataggcca acaacacgct
    24601 cggaccggcc attttgatgg cgtctgccga accgtaaaac agcccggtac caattgctga
    24661 acccagtgcc ataaagcgta tgtggcgggt acttagccca cgctttagct tgttcttact
    24721 ttccatcaat tcctaaccca tcaacacaat aaaaaaccac ggggcatcgc agccccgtgg
    24781 ttaaaacaaa tgttcagtga tttagtgagc gctggaggtc acctgacgac ctgccgcgcg
    24841 atcccagata atggccagaa ccaccatcac cactgttggc attaaccacg ccagaccttg
    24901 ttcggccagc ggtaaacgct gcgcccagga cggtaagata tcgctgaatg cagatgcttt
    24961 gatcccgtcg agaataccaa aaagcaggct gataaacatc ggcggagcaa tcacgcggga
    25021 cgaattatgc caccatgagc gtgtaaaact taatacaacc agtgcgatac acggcggata
    25081 aatagcggtc agcaccggta cggagatctg gatcagctgg cttaagccga ggttagaaac
    25141 caccatcgag aagccgccga ggataaacac cagcgtacga taagagagcg gtacgtactg
    25201 ggcaaagaat tctgcacaag cacaggtcag gccaactgcc gttaccaagc aggcgatgaa
    25261 gattaacgcc gccagcagga agctaccgcc gccgccaaag gtgtgctgaa cgtaagcatg
    25321 cagaatagca gcgccgtttg cagactgatc gaccagcgac gcgctgtctg aacccagacg
    25381 gaacagcgcc aggtacagca gagtcagacc aacacccgcc atcaggccag cccagacggt
    25441 ataacgggtc agcagacgcg cttcggtaac gccacgagaa cgcgccgcgt taacaataac
    25501 gataccaaac accattgcgc ccagcgtatc catggtcaga tagccgttaa cgaagccgtt
    25561 agaaaacgca gcgttttgat aagcctcagt cgccgtgctg atagaacccg ccggccagac
    25621 aatcgcggca acagacagga tgaccagcgc gataattttc agcggcgcaa ggaagttgcc
    25681 cacggtatcc agcagcttgc ccggatagag cgaaaccaga ataacgatag cgaaatagac
    25741 caggctgtag ataaacagcg gcagcgcgga atcaccagtc agcggcgcaa tccccacttc
    25801 aaaggaaacg gtagctgtac gcggcgtagc gaaaagcggc cccaccgcca ggtaacaaac
    25861 cgttgccagc agcacgccag cgactttacc gattggcgtg ctgaggctgt caacaccgcc
    25921 gccaactttt gccagcgcca ctaccgttaa taccggcagg ccaacggcag taatgaggaa
    25981 gccgaatgcc gcagtccaga cgtgttcgcc agcctgcaag ccgaccattg gagggaaaat
    26041 aatgttacct gcgccgacga acaacgcaaa tgtcataaag cccagagcga tgatatcgcg
    26101 cgatcttaat tgatgggtca taaatcatac tgcctgtgga tgtggtgttg taacgttaaa
    26161 tttttgtgcc aatccctggg gatgatacag cggtatattt aaacaatttt agcgggaaat
    26221 actcccaacc aggcgcggcg aagaatagtt tttcgattta ccgcgttaat gcagtcagta
    26281 tgacagcacc tgaagcgcaa tttaaacgct tataacgttt taaagcaagg agggatctaa
    26341 aaaccagagg tatatagcga taagaaatca ccaaccagta taaaacacta tttccgtgaa
    26401 aaagacatgg ctaatcatgc gaacaaaaaa tcaccaaccg ttgtaaaatc taacgcccgt
    26461 attttgactg caaagaaaat aagccagcat cgactggctt atggcaataa aagatgacaa
    26521 aggcggatta atcgctgttt ttggcaatta aacgttccgg gataacaaaa ctgaaacgtg
    26581 ttccttttcc tactgtactc tcaatattca ggcgactttc gtgatgattc acagcatgtt
    26641 tcacgatcgc taaccctaat ccgctaccgc cggtttgccg ggaacgcgct ttatcaacgc
    26701 gataaaaacg ctcggtcagg cgcggaatat gctccggtgc aatgcccggt ccgttatctt
    26761 caacgctaaa ttcggcaccg tgcggcactc gctgccagcg tacggtgata tgcgtgcctt
    26821 ctggagtatg attcacggcg ttatagacca ggttcgaaat cgcactgcgt agctgatctt
    26881 cgttgccaga cactttgagg ccgttatcta tctcaaaggt aaatgtctgt tttttctgac
    26941 tcagagtctg agcctcgcgc tcaacaacgc gcagcatcat cggcacatca accttttcat
    27001 tgagcaattg cgtcggcgcg gcttcaattt tcgacagcgt tagcaactgc ttcaccagcc
    27061 cttccatccg ctgggtctgc tcgcgcatgg tgtgcaacgc tttttcgcgt accgcgcctt
    27121 ccagcggctg ctcatccatc atctccaggt aaccctgtaa cacggtcaat ggcgtacgta
    27181 actcatggct cacgttggca aaaaagttac gccgcgcccc ttccagttga tgcatttgcg
    27241 tgacatcacg cgccaccatc agcaactgtt tgtgggtata aggcatgacg cgaatttcca
    27301 gatgccgccc ggtgttgagc accagattga gcgggcgaga aaaatcacgc gttttcagat
    27361 attgcgtaaa ctccgggtaa cgcagtaggt taaggatgtt ctgcccgtta tcttccggcc
    27421 agcgcaaacc aagaatttgt tgcgccagac cgttacacca gaaaataccg ctctcttccg
    27481 tggtcagcac caccgcgtcg ggcagcgact ccgcgccgct acgaaagcgt ttaatcagat
    27541 tgcccagttc acggcggcgt tttttatttc gcagctgcat ctggtgtaag ccgtatagta
    27601 gcggttccca gctaccacgc cccggtggcg gggtcatact gcgatccacc cacagccacc
    27661 atgaaaggcg caataaattc cagaaatgcc agataagcag tcctgttacc gatgccagca
    27721 aaaaccaggg caggtaacca aaaaatgcac ccaggatgaa agccgggagg cagcaaagta
    27781 gcagctccag caccagcctt ttccacgaca gccgttccag cacgtaagat actcccgtta
    27841 agaaatcata agccctgctc tgcgtccgat gagcaaggcg ttaaaagcgg gttgaaaaac
    27901 gatatcctgt accgcgcacg gtctgcacca tgcggtcatg cccgccgggc tccagtgctt
    27961 tacgcaggcg acgaatgtgg acatcgaccg tgcggtcttc cacatacacg ttagttcccc
    28021 agacgtggtt taacagctgc tcgcggctgt acacgcgctc aggatgcgtc ataaaaaagt
    28081 gcagcagttt aaattctgtc ggccccatct ccagcggctc ttcgcccgcc atcactcggt
    28141 gagatgtcgg gtcgagactt aatccctgca tctcaatcac ctcttccacc gccattggcg
    28201 aaatacggcg cattaccgct ttgattcgcg ccaccagctc cttcggcgaa aacggcttgg
    28261 tgatatagtc atccgcgccg gtttcaaggc cgcgcacgcg atcttcttct tcccctctgg
    28321 cggtcaacat caccactgga atatcccggg tcatcgactc gcgcttaagg tgtttgatga
    28381 actggatacc ggagccgcca ggtaacatcc agtcaaggag aattaaatcc ggccagggtt
    28441 cattcagttg attcacagca ctgtcataat cttccgcttc gaccggctga aagccatttt
    28501 gttcgagcac gaagcagacc atttcacgaa ttggagcttc atcttctacg accagaatac
    28561 gtctcgccat gatttgccct gttgtaataa ataggttgcg atcattaatg cgacgtcatt
    28621 atgcgtcaga tttatgacag atttatgaaa agctcgtcgc acatatcttc aggttattga
    28681 tttccgtggc gcagaaaaaa gcaaatggca catctgtttg ggtataatcg cgcccatgct
    28741 ttttcgcctg ggaaccgtta tgcgcatcct tcacacctca gactggcatc tcggccagaa
    28801 cttctacagt aaaagccgcg aagctgaaca tcaggctttt cttgactggc tgctggagac
    28861 aacacaaacc catcaggtgg atgcgattat tgttgccggt gatgttttcg ataccggctc
    28921 gccgcccagt tacgcccgca cgttatacaa ccgttttgtt gtcaatttac agcaaactgg
    28981 ctgtcatctg gtggtactgg caggaaacca tgactcggtc gccacgctga atgaatcgcg
    29041 cgatatcatg gcgttcctca atactaccgt ggtcgccagc gccggacatg cgccgcaaat
    29101 cttgcctcgt cgcgacggga cgccaggcgc agtgctgtgc cccattccgt ttttacgtcc
    29161 gcgtgacatt attaccagcc aggcggggct taacggtatt gaaaaacagc agcatttact
    29221 ggcagcgatt accgattatt accaacaaca ctatgccgat gcctgcaaac tgcgcggcga
    29281 tcagcctctg cccatcatcg ccacgggaca tttaacgacc gtgggggcca gtaaaagtga
    29341 cgctgtgcgt gacatttata ttggcacgct ggacgcgttt ccggcacaaa actttccacc
    29401 agccgactac atcgcgctcg ggcatattca ccgcgcacag attattggcg gcatggaaca
    29461 tgttcgctat tgcggctccc ccattccact gagttttgat gaatgcggta agagtaaata
    29521 tgtccatctg gtgacatttt caaacggcaa attagagagc gtggaaaacc tgaacgtacc
    29581 ggtaacgcaa cccatggcag tgctgaaagg cgatctggcg tcgattaccg cacagctgga
    29641 acagtggcgc gatgtatcgc aggagccacc agtctggctg gatatcgaaa tcactactga
    29701 tgagtatctg catgatattc agcgcaaaat ccaggcatta accgaatcat tgcctgtcga
    29761 agtattgctg gtacgtcgga gtcgtgaaca gcgcgagcgt gtgttagcca gccaacagcg
    29821 tgaaaccctc agcgaactca gcgtcgaaga ggtgttcaat cgccgtctgg cactggaaga
    29881 actggatgaa tcgcagcagc aacgtctgca gcatcttttc accacgacgt tgcataccct
    29941 cgccggagaa cacgaagcat gaaaattctc agcctgcgcc tgaaaaacct gaactcatta
    30001 aaaggcgaat ggaagattga tttcacccgc gagccgttcg ccagcaacgg gctgtttgct
    30061 attaccggcc caacaggtgc ggggaaaacc accctgctgg acgccatttg tctggcgctg
    30121 tatcacgaaa ctccgcgtct ctctaacgtt tcacaatcgc aaaatgatct catgacccgc
    30181 gataccgccg aatgtctggc ggaggtggag tttgaagtga aaggtgaagc gtaccgtgca
    30241 ttctggagcc agaatcgggc gcgtaaccaa cccgacggta atttgcaggt gccacgcgta
    30301 gagctggcgc gctgcgccga cggcaaaatt ctcgccgaca aagtgaaaga taagctggaa
    30361 ctgacagcga cgttaaccgg gctggattac gggcgcttca ctcgttcgat gctgctttcg
    30421 caggggcaat ttgctgcctt cctgaatgcc aaacccaaag aacgcgcgga attgctcgag
    30481 gagttaaccg gcactgaaat ctacgggcaa atctcggcga tggtttttga gcagcacaaa
    30541 tcggcccgca cagagctgga gaagctgcaa gcgcaggcca gcggcgtcac gttgctcacg
    30601 ccggaacaag tgcaatcgct gacagcgagt ttgcaggtac ttactgacga agaaaaacag
    30661 ttaattaccg cgcagcagca agaacaacaa tcgctaaact ggttaacgcg tcaggacgaa
    30721 ttgcagcaag aagccagccg ccgtcagcag gccttgcaac aggcgttagc cgaagaagaa
    30781 aaagcgcaac ctcaactggc ggcgcttagt ctggcacaac cggcacgaaa tcttcgtcca
    30841 cactgggaac gcatcgcaga acacagcgcg gcgctggcgc atattcgcca gcagattgaa
    30901 gaagtaaata ctcgcttaca gagcacaatg gcgcttcgcg cgagcattcg ccaccacgcg
    30961 gcgaagcagt cagcagaatt acagcagcag caacaaagcc tgaatacctg gttacaggaa
    31021 cacgaccgct tccgtcagtg gaacaacgaa ctggcgggtt ggcgtgcgca gttctcccaa
    31081 caaaccagcg atcgcgagca tctgcggcaa tggcagcaac agttaaccca tgctgagcaa
    31141 aaacttaatg cgcttgcggc gatcacgttg acgttaaccg ccgatgaagt tgctagcgcc
    31201 ctggcgcaac attccgagca acgcccactg cgtcagcgcc tggtcgcgct gcatgggcag
    31261 attgttcccc aacaaaaacg tctggcgcag ttaatggtca ctatccagaa tgtcactctg
    31321 gaacagacgc aacgtaatgt cgcacttaac gaaatgcgcc agcgttataa agaaaagacg
    31381 cagcaacttg ccgatgtgaa aaccatttgc gagcaggaag cgcgcatcaa aacgctggaa
    31441 gctcaacgtg cacagttaca ggcgggtcaa ccttgtccac tttgtggttc caccagccac
    31501 ccggcggtcg aggcgtatca ggcgctggag cctggtgtta atcagtctcg attactggcg
    31561 ctggaaaacg aagttaaaaa gctcggcgaa gaaggtgcgg cgctgcgcgg gcaactggat
    31621 gcattaacga agcagcttca gcgcgatgaa aacgaagcgc aaagcctccg acaagatgag
    31681 caagcactta ctcaacaatg gcaagccgtc acgaccagcc tcaatatcac cttgcagcca
    31741 caggacgata ttcaaccgtg gctggatgca caagatgagc acgaacgcca gctgcggtta
    31801 ctcagccaac gacatgaatt acaagggcag attgccgcgc ataatcagca aattatccag
    31861 tatcaacagc aaattgaaca acgccagcag cagctcttaa cggcattgac gggttatgca
    31921 ctgacattgc cacaggaaga tgaagaagag agctggttgg cgacacgtca gcaagaagcg
    31981 cagagctggc agcaacgcca gaacgaatta accgcgctgc aaaaccgtat tcagcagctg
    32041 acgccgattc tggaaacgtt gccgcaaagt gatgatctcc cgcacagcga agaaactgtg
    32101 gcgctggata actggcggca ggttcatgaa caatgtctcg cattacacag ccagcagcag
    32161 acgttacagc aacaggatgt tctggcggcg caaagtctgc aaaaagccca ggcgcagttt
    32221 gacaccgcgc tacaggccag cgtctttgac gatcagcagg cgttccttgc ggcgctaatg
    32281 gatgaacaaa cactaacgca gctggaacag ctcaagcaga atctggaaaa ccagcgccgt
    32341 caggcgcaaa cgctggtcac acagacagca gaaacgctgg cacagcatca acaacaccga
    32401 cctgacgggt tggctctcac tgtgacggtg gagcagattc agcaagagtt agcgcaaact
    32461 caccaaaagt tgcgtgaaaa caccacgagt caaggcgaga ttcgccagca gctgaagcag
    32521 gatgcagata accgtcagca acaacaaacc ttactgcagc aaattgctca aatgacgcag
    32581 caggttgagg actggggata tctgaattcg ctaataggtt ccaaagaggg cgataaattc
    32641 cgcaagtttg cccaggggct gacgctggat aatttagtcc atctcgctaa tcagcaactt
    32701 acccggctgc acgggcgcta tctgttacag cgcaaagcca gcgaggcgct ggaagtcgag
    32761 gttgttgata cctggcaggc agatgcagta cgcgataccc gtaccctttc cggcggcgaa
    32821 agtttcctcg ttagtctggc gctggcgctg gcgctttcgg atctggtcag ccataaaaca
    32881 cgtattgact cgctgttcct tgatgaaggt tttggcacgc tggatagcga aacgctggat
    32941 accgcccttg atgcgctgga tgccctgaac gccagtggca aaaccatcgg tgtgattagc
    33001 cacgttgaag cgatgaaaga gcgtattcct gtgcagatca aagtgaagaa gatcaacggc
    33061 ctgggctaca gcaaactgga aagtacgttt gcagtgaaat aactattcag caggataatg
    33121 aatacagagg ggcgaattat ctcttggcct tgctggtcgt tatcctgcaa gctatcactt
    33181 tattggctac ggtgattggt agccgttctg gtggttgtga tggtggtatg aaaaaagtca
    33241 ttttatcttt ggctctgggc acgtttggtt tggggatggc cgaatttggc attatgggcg
    33301 tgctcacgga gctggcgcat aacgtaggaa tttcgattcc tgctgccggg catatgatct
    33361 cgtattatgc actgggggtg gtggtcggtg cgccaatcat cgcactcttt tccagccgct
    33421 actcactcaa acatatcttg ttgtttctgg tggcgttgtg cgtcattggc aacgccatgt
    33481 tcacgctctc ttcgtcttac ctgatgcttg ccattggtcg cctggtttcc ggctttccgc
    33541 atggcgcatt ttttggcgtc ggcgcgatcg tgttatcaaa aattatcaaa cccggaaaag
    33601 tcaccgccgc cgtggcgggg atggtttccg ggatgacagt cgccaatttg ctgggcattc
    33661 cgctgggaac gtatttaagt caggaattta gctggcgtta caccttttta ttgatcgctg
    33721 tttttaatat tgcggtgatg gcatcggtct atttttgggt gccagatatt cgcgacgagg
    33781 cgaaaggaaa tctgcgcgaa caatttcact ttttgcgcag cccggccccg tggttaattt
    33841 tcgccgccac gatgtttggc aacgcaggtg tctttgcctg gttcagctac gtaaagccat
    33901 acatgatgtt tatttccgtt ttttcggaaa cggcgatgac ctttattatg atgttagttg
    33961 ggctagggat ggtgctggga aatatgctaa gtggcaggat ttcaggacgt tattcaccac
    34021 tgcgcattgc agcagtgact gactttatca ttgtactggc actgctgatg ctctttttct
    34081 gcggcggcat gaaaacaacg tcgcttattt ttgcttttat ttgttgcgcg ggattatttg
    34141 ccctttcagc acctctgcaa atattgttac tgcaaaacgc caaaggcgga gagttattag
    34201 gtgccgcagg tgggcaaata gcgtttaacc tcggtagcgc cgtcggcgca tattgcggag
    34261 gtatgatgct gacgctgggg ctggcatata attacgtggc gctgcctgcc gccctgcttt
    34321 cgtttgctgc gatgtcatcg ttgctgctgt atggtcgcta taagcgccag caagcggcgg
    34381 atagtccggt gctggcgaaa ccactggggt agattactgt cacggtgcgc gttgcttatt
    34441 gccggatgcg gcgtgaacgc cttatccgcc ctacgcggtt ctggcacatt ttgtaggcct
    34501 gataagacgc ggcaagcgtc gcatcaggca tgatgcgcca attgcctacg ttttttactc
    34561 ttgtggccat aaccacgcag cgccgcgtac gccgctggaa tcaccgtgct tcgccttacg
    34621 caccggcgtt tcacattcgc cgccgaagac aaattgttta atcaactgcc caaccgtttg
    34681 atataaacgg tctacattgc tcatcccgcc ccccaggaca atcacatccg gatcgagaat
    34741 attcacgaca tgtgccagcg attttgccag ccgcagctcg tagcgacgca atgccagttc
    34801 cgctaccgga tcgctttctt caaccaggcg gataatttca ctgcctttca gcgcatgtcc
    34861 gctcaaacga cgataatccg tcgcgaatcc cgtgcccgaa ataaaggttt caatacaacc
    34921 ttgtttaccg caataacaag ggacttcctc gcgataacgc agctcgtctt catccatcca
    34981 cggtagcgga ttgtgtcccc actcgcctgc cgtgccattg ccgccgatat gcgcccgccc
    35041 atttaatgcc acgcccgcgc cgcatcccgt gccgataatc acggcaaata ccgtctgcgc
    35101 tcccgctgcc gcgccatcta ctgcttctga aaccgccaga cagttggcat catttgccag
    35161 ccgcacttcc cgctgcaacc tcgcgcttaa gtctttatcg aatggctgac cgttgagcca
    35221 ggttgaattg gcattcttca ccacaccggt gtagggcgaa atcgaacctg gaatgcccat
    35281 acctaccgtt ccgcgctgcc ctgtcgcctg ctccgccata tcaaccaacg tggcgatcgt
    35341 ttcaatagtc tgccggtaat catcacgcgg cgtgggcaga cgatggcggt acaactgctc
    35401 ccctgcatcg cccagtgcaa tcacttcagt tttggtgccg cctaaatcga tacctatacg
    35461 cactgtactc tccttatttt tttcaatatc aatagcgtag agacggacaa ccggattggc
    35521 aatgcaaggc cgccgacaat tcgttatcat gcccgctaaa tttaacgaca aggccgtgga
    35581 aattatcatg ctgtggttca aaaatttaat ggtttaccgt cttagccgcg agatttcgct
    35641 gcgtgcagaa gagatggaaa aacagctagc ctcgatggca tttaccccat gcggcagcca
    35701 ggacatggcg aagatgggct gggttcctcc gatgggatcg cacagcgatg cgttaacgca
    35761 cgttgccaat ggtcaaattg ttatctgcgc gcgcaaagaa gaaaaaatcc tcccgtctcc
    35821 ggtgattaaa caggcgctgg aagcgaaaat cgccaaactg gaagcggaac aggcgcgtaa
    35881 gctgaagaaa accgaaaaag attcgctgaa agacgaagtg ctgcactctc tgctgccgcg
    35941 tgctttcagc cgttttagcc agacaatgat gtggatcgac acggttaacg gtttgattat
    36001 ggtggactgc gccagtgcca aaaaagcgga agatacgctg gcattactgc gtaaaagcct
    36061 ggggtcgtta ccggttgtac cattgagcat ggaaaacccg attgaactga cgctgaccga
    36121 atgggttcgc tccggtagtg cggcacaggg cttccagctg cttgatgagg ccgagctgaa
    36181 atcgttgctg gaagatggcg gcgtgatccg cgcgaagaaa caagatctga ccagcgaaga
    36241 gatcaccaat cacattgaag ccggaaaagt ggtgactaaa ctggcgctcg actggcagca
    36301 gcgcattcag tttgtgatgt gcgacgatgg ttcgctcaag cgtcttaagt tctgcgacga
    36361 gctgcgcgat caaaacgaag atatcgaccg tgaagatttc gccctgcgtt ttgatgccga
    36421 tttcatcctg atgactggtg aactggcagc gttaattcaa aacctgattg aaggattagg
    36481 tggcgaagca caacgttaat tgctgatttt cctttaatgc cggatgcgac gcctgccgcg
    36541 tcttatccgg cgtacgaagc cacaccaggc atataattat tcgctacggc gagcaataat
    36601 ttttagcgca gcaatattat gcgttttacg ccgtaactga tctaatcgcc tcatccctat
    36661 tcgcctgtca gcagcacgca gaatattcac taacccccat ttatcgctgg taaattgttt
    36721 tgttaacagc tccgcttttg gggtatcaat atattcacgc aataaatcag aaattgaaca
    36781 aatatccgtc aggtaaggat accagcaagt ttcaccgtga aaattacgca ctccagcgtt
    36841 acctgcaata aagtctttcg ggtaatccca gatcacgttt ttatccaacg tgatccaata
    36901 acgcggtaaa tcggtacttc caccattttg gctgcgcatg agataacggg tacagtgaat
    36961 ttgcaggttg atcgttggcg tcagtaagtc atagatctcc cgctgtaact tgctccatgg
    37021 atgttgtgtc attgtttttc ctcaagccgg tgacgcgctt cactgcgaat tttccgcgtt
    37081 gccccggttt tcactaaatc acgtaactgc gaaagatcgg gttgttcgac aaataagagt
    37141 aatgatagat cctcttttaa ccacgccgtt ttcacatcgg cagccagctg tggattatta
    37201 atagccagcg accgatcctg agactcagtc aatgtcgtta acaacgacgc cggggtatgt
    37261 gcgttgcgta atgccgtttg gcgcaaatga tcattgcgac tttgcagcaa tgcacgccac
    37321 cattcatcat ctgatttatc ttttatcgta gccagttccg caaaaaatac ctggagatct
    37381 tcactgatat ttatatcaac cttgatgaat tatgaatata tcggaaattg aaaaaatcgc
    37441 agcaaagga
//
LOCUS       DAJVRF010000024        36258 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJVRF010000024 DAJVRF010000000
VERSION     DAJVRF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36258
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.024"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            351..1799
                     /gene="uacT"
                     /locus_tag="OQN13_001113"
     CDS             351..1799
                     /gene="uacT"
                     /locus_tag="OQN13_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCS1614531.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPFIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFLHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1801..1926
                     /locus_tag="OQN13_001114"
     CDS             1801..1926
                     /locus_tag="OQN13_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614532.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1923..1994)
                     /locus_tag="OQN13_001115"
     CDS             complement(1923..1994)
                     /locus_tag="OQN13_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614533.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            2049..2597
                     /gene="idi"
                     /locus_tag="OQN13_001116"
     CDS             2049..2597
                     /gene="idi"
                     /locus_tag="OQN13_001116"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCS1614534.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(2640..4157)
                     /gene="lysS"
                     /locus_tag="OQN13_001117"
     CDS             complement(2640..4157)
                     /gene="lysS"
                     /locus_tag="OQN13_001117"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCS1614535.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(4167..5265)
                     /gene="prfB"
                     /locus_tag="OQN13_001118"
     CDS             complement(join(4167..5189,5191..5265))
                     /gene="prfB"
                     /locus_tag="OQN13_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCS1614536.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5356..7089)
                     /gene="recJ"
                     /locus_tag="OQN13_001119"
     CDS             complement(5356..7089)
                     /gene="recJ"
                     /locus_tag="OQN13_001119"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCS1614537.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(7095..7805)
                     /gene="dsbC"
                     /locus_tag="OQN13_001120"
     CDS             complement(7095..7805)
                     /gene="dsbC"
                     /locus_tag="OQN13_001120"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCS1614538.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(7830..8726)
                     /gene="xerD"
                     /locus_tag="OQN13_001121"
     CDS             complement(7830..8726)
                     /gene="xerD"
                     /locus_tag="OQN13_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCS1614539.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8838..9359
                     /gene="fldB"
                     /locus_tag="OQN13_001122"
     CDS             8838..9359
                     /gene="fldB"
                     /locus_tag="OQN13_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCS1614540.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9399..9806)
                     /locus_tag="OQN13_001123"
     CDS             complement(9399..9806)
                     /locus_tag="OQN13_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HCS1614541.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9787..10053)
                     /gene="sdhE"
                     /locus_tag="OQN13_001124"
     CDS             complement(9787..10053)
                     /gene="sdhE"
                     /locus_tag="OQN13_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCS1614542.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10296..11276
                     /gene="ygfZ"
                     /locus_tag="OQN13_001125"
     CDS             10296..11276
                     /gene="ygfZ"
                     /locus_tag="OQN13_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCS1614543.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(11472..12131)
                     /locus_tag="OQN13_001126"
     CDS             complement(11472..12131)
                     /locus_tag="OQN13_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCS1614544.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(12295..12606)
                     /locus_tag="OQN13_001127"
     CDS             complement(12295..12606)
                     /locus_tag="OQN13_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCS1614545.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            12651..14084
                     /gene="bglA"
                     /locus_tag="OQN13_001128"
     CDS             12651..14084
                     /gene="bglA"
                     /locus_tag="OQN13_001128"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCS1614546.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            complement(14141..14884)
                     /locus_tag="OQN13_001129"
     CDS             complement(14141..14884)
                     /locus_tag="OQN13_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1614547.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVFSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(15150..18023)
                     /gene="gcvP"
                     /locus_tag="OQN13_001130"
     CDS             complement(15150..18023)
                     /gene="gcvP"
                     /locus_tag="OQN13_001130"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCS1614548.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(18142..18531)
                     /gene="gcvH"
                     /locus_tag="OQN13_001131"
     CDS             complement(18142..18531)
                     /gene="gcvH"
                     /locus_tag="OQN13_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCS1614549.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(18555..19649)
                     /gene="gcvT"
                     /locus_tag="OQN13_001132"
     CDS             complement(18555..19649)
                     /gene="gcvT"
                     /locus_tag="OQN13_001132"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCS1614550.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            19939..20022
                     /locus_tag="OQN13_001133"
     CDS             19939..20022
                     /locus_tag="OQN13_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614551.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            complement(20096..21298)
                     /gene="ubiI"
                     /locus_tag="OQN13_001134"
                     /gene_synonym="visC"
     CDS             complement(20096..21298)
                     /gene="ubiI"
                     /locus_tag="OQN13_001134"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCS1614552.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(21321..22499)
                     /gene="ubiH"
                     /locus_tag="OQN13_001135"
                     /gene_synonym="visB"
     CDS             complement(21321..22499)
                     /gene="ubiH"
                     /locus_tag="OQN13_001135"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCS1614553.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(22496..23821)
                     /gene="pepP"
                     /locus_tag="OQN13_001136"
     CDS             complement(22496..23821)
                     /gene="pepP"
                     /locus_tag="OQN13_001136"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCS1614554.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNELEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(23847..24425)
                     /gene="ygfB"
                     /locus_tag="OQN13_001137"
     CDS             complement(23847..24425)
                     /gene="ygfB"
                     /locus_tag="OQN13_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCS1614555.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24593..24922
                     /gene="zapA"
                     /locus_tag="OQN13_001138"
     CDS             24593..24922
                     /gene="zapA"
                     /locus_tag="OQN13_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCS1614556.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            24964..25147
                     /gene="ssrS"
                     /locus_tag="OQN13_001139"
     ncRNA           24964..25147
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OQN13_001139"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            25168..25770
                     /locus_tag="OQN13_001140"
     CDS             25168..25770
                     /locus_tag="OQN13_001140"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCS1614557.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(25871..25930)
                     /locus_tag="OQN13_001141"
     CDS             complement(25871..25930)
                     /locus_tag="OQN13_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1614558.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26262..26321)
                     /gene="ibsC"
                     /locus_tag="OQN13_001142"
     CDS             complement(26262..26321)
                     /gene="ibsC"
                     /locus_tag="OQN13_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCS1614559.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(26550..27782)
                     /gene="serA"
                     /locus_tag="OQN13_001143"
     CDS             complement(26550..27782)
                     /gene="serA"
                     /locus_tag="OQN13_001143"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCS1614560.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(28038..28697)
                     /gene="rpiA"
                     /locus_tag="OQN13_001144"
     CDS             complement(28038..28697)
                     /gene="rpiA"
                     /locus_tag="OQN13_001144"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCS1614561.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            29127..30020
                     /gene="argP"
                     /locus_tag="OQN13_001145"
     CDS             29127..30020
                     /gene="argP"
                     /locus_tag="OQN13_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCS1614562.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            30223..32367
                     /gene="scpA"
                     /locus_tag="OQN13_001146"
     CDS             30223..32367
                     /gene="scpA"
                     /locus_tag="OQN13_001146"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCS1614563.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            32360..33355
                     /gene="meaB"
                     /locus_tag="OQN13_001147"
     CDS             32360..33355
                     /gene="meaB"
                     /locus_tag="OQN13_001147"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCS1614564.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            33366..34151
                     /gene="scpB"
                     /locus_tag="OQN13_001148"
     CDS             33366..34151
                     /gene="scpB"
                     /locus_tag="OQN13_001148"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCS1614565.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            34175..35653
                     /gene="scpC"
                     /locus_tag="OQN13_001149"
     CDS             34175..35653
                     /gene="scpC"
                     /locus_tag="OQN13_001149"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCS1614566.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(35650..>36258)
                     /locus_tag="OQN13_001150"
     CDS             complement(35650..>36258)
                     /locus_tag="OQN13_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1614567.1"
                     /translation="IGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDR
                     QLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRF
                     QNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPAT
                     LKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         8682 a         9729 c         9156 g         8691 t
ORIGIN      
        1 aattataatt ttgcgactga gttcaaatta ttgcccctca atctatgccg ctttcattat
       61 ttcaacattg ccgatcaaaa gagcgctatc cagataaaaa cgattaaatg cgagagtgcg
      121 acatgccaga atgattaatg aaatatcaat atgagaaaat tacagataat tccattgtca
      181 ggttctcttg tcattttcgt ggtttaagca cgattatgtg attgcctccg caattcctgt
      241 ctctaactcc ccttcctcgc aaaaactggc actccacgag catgtgttta gacagtttca
      301 ttaacgtaaa cggttgcttt ttactctggc gggcgaaagg agaaacactg atgagcgcca
      361 tagattccca acttccctca tcttctgggc aagaccgccc aactgatgag gttgaccgca
      421 tattatcacc aggaaagctg atcatactcg gtctgcaaca cgtccttgtc atgtacgcag
      481 gtgcagtcgc tgttcctctt atgattggtg accgactggg cctctcaaaa gatgctattg
      541 cgatgctcat tagctcggat ctcttttgct gcgggatcgt cacattattg caatgtatcg
      601 gtatcggccg ctttatgggg atccgcctgc cggtgattat gtcggtgacc tttgctgctg
      661 taacaccaat gatagccatt gggatgaacc cggatatcgg cctgctgggg atatttggtg
      721 ccactatcgc cgcgggtttt atcaccacat tattagcgcc atttattggt cgcttgatgc
      781 ctttattccc gccactggtt accggtgtgg ttattacttc tattgggctt agcatcattc
      841 aggtgggtat tgactgggcc gccggaggta aagggaatcc gcaatatggt aatcccgttt
      901 atttaggtat ctcctttgct gtcttaattt ttatcttgct cattactcgc tatgcgaaag
      961 gatttatgtc caacgtcgcc gtattactgg ggattgtatt tggcttttta ctttcgtgga
     1021 tgatgaatga agtcaattta tccgggctac atgatgcttc atggtttgcg attgtcacgc
     1081 cgatgtcatt tggtatgccg attttcgatc ccgtttccat tctgaccatg actgccgtgt
     1141 taatcatcgt gtttatcgag tcgatgggga tgttcctggc actgggtgaa atagtcggtc
     1201 gtaaactctc ttcacacgat attattcgcg ggctgcgtgt cgatggcgta gggacaatga
     1261 taggcggaac gtttaacagc ttcctccaca cgtcattttc acaaaacgtt ggcctggtta
     1321 gcgtgacgcg cgttcatagc cgctgggtgt gtatttcttc gggaattata ttaatcctgt
     1381 ttggcatggt gccaaaaatg gcggtgctgg tcgcctccat tccgcaattt gtgctgggcg
     1441 gtgctgggct ggtgatgttc ggcatggtac tggcgacagg gattcgaatt ctgtcgcgct
     1501 gtaactacac caccaaccgt tacaacctct atattgtggc gatcagtctc ggcgttggca
     1561 tgactccgac gctctctcac gatttctttt ctaagttacc ggccgtactg caaccgttgc
     1621 tgcatagcgg cattatgctc gcaaccctta gcgccgttgt gctgaatgtc ttctttaatg
     1681 gctaccagca tcatgctgac ctggtgaagg aatccgtctc tgataaagat ttaaaagtca
     1741 ggacagtacg tatgtggctt ctgatgcgca agctgaagaa aaatgagcat ggagaataat
     1801 atgaattttt taatgcgcgt tatattcagt ctgctgttgc tttttactct ctctattcct
     1861 gtcatttctg actgtgttgc aatggccatt gaaagtcgct tcaaatatat gatgctactt
     1921 ttttaaatgg tttttacctg tcggcatccg ctcaaaacgg gcggttgtcg ataaacgctc
     1981 acttggttaa tcatttcact cttcaattat ctataatgat gagtgatcag aattacatgt
     2041 gagaaattat gcaaacggaa cacgtcattt tattgaatgc acagggagtt cccacgggta
     2101 cgctggaaaa gtatgccgca cacacggcag acacccgctt acatctcgcg ttctccagtt
     2161 ggctgtttaa tgccaaagga caattattag ttacccgccg cgcactgagc aaaaaagcgt
     2221 ggcctggcgt gtggactaac tcggtttgtg ggcacccaca actgggagaa agcaacgaag
     2281 acgcggtgat ccgccgttgc cgttatgagc ttggcgtgga aattacgcct cctgaatcta
     2341 tctatcctga ctttcgctat cgcgccaccg atccgaatgg cattgtggaa aatgaagtgt
     2401 gtccggtatt tgccgcacgc accaccagtg cgttacagat caacgatgat gaagtgatgg
     2461 attatcaatg gtgtgattta gcagatgtat tacacggtat tgatgccaca ccgtgggcgt
     2521 tcagtccgtg gatggtgatg caggcagcca atagtgaagc aagaaaattg ttgtctgctt
     2581 tcgcgcagca caattaaaaa accccgacat ttgccggggt tgtgagcata acgtaatgct
     2641 tattttaccg gacgcatcgc cgggaacaga ataacgtcgc ggatggtatg gctgttggtg
     2701 aacagcatta ccatacggtc gatgccaatc cccagacctg ctgtcggcgg taagccatgt
     2761 tccagtgcgg taacgtaatc ttcatcgtag aacatcgctt cgtcgtcacc tgcgtctttc
     2821 gcggcaacct gatccaggaa gcgttgcgcc tgatcttccg cgtcattcag ctcgctaaag
     2881 ccgttaccga tttcacgacc accgatgaag aactcaaagc ggtctgtgat ttccgggtta
     2941 atgtcgttac gacgcgccag cggagaaact tctgccggat attcagtaat gaaggtcggc
     3001 tgaatcagat gtgcttctgc cacttcttcg aagatctcgg taacgatacg gcccagaccc
     3061 cagctcttct caacgtggat accgatagat tcagcaatcg ctttcgcaga gtcgaagtta
     3121 tccagatccg ccatgtcggt ttccgggcga tatttcttga tcgcttcacg catggtcagt
     3181 ttttcgaacg gtttaccgaa gtccagcgtc acgtcgccgt aggtcacttc cgtcttaccg
     3241 agaatatcct gtgccagagt acggaacagc gattcggtca gctcgatcag atctttgtaa
     3301 tctgcgtaag ccatgtagag ttccatcatg gtgaactctg ggttatgacg tacggaaata
     3361 ccttcgttac ggaagttacg gttgatttcg aatacacgct cgaagccacc gaccaccaga
     3421 cgcttgaggt acagttccgg cgcgatacgc aggtacatgt cgagatccag cgcgttatgg
     3481 tgggtgataa acggacgcgc agcggcaccg cctgggatca cctgcatcat cggcgtttca
     3541 acttccataa agccgcggtt caccatgaac tggcgaatac cagagaggat ctgcgagcgt
     3601 actttaaagg tgttacggga ttcatcgtta gagatgagat ccagataacg ctgacgatag
     3661 cgcgcttcct gatcctgcaa gccgtggaat ttatccggca gcggacgcag tgctttggtc
     3721 agcagacgca gctcggtaca gtggatagac agttcgccgg ttttggtttt gaacagttta
     3781 cctttcgcgc cgaggatgtc gccgaggtcc cattttttga actgctcgtt gtaaacgcct
     3841 tccggcagat cgtcacgggc aacgtacagc tgaatgcggc cacccacatc ctgcagggta
     3901 acgaaagacg ctttacccat aatacggcgg gtcatcatgc ggccagcaac ggcgacttcg
     3961 atgttcagcg cttccagttc ttcgttctct ttaccgtcga attctgcgtg caattggtca
     4021 gaggtatgat cgcgacggaa atcgttcggg aaggcaatcc cctgctcgcg caggttcgcc
     4081 agcttctcac gacgcgtttt cagttcattg ttaagatcga ctaccgcgtc agcgccctgt
     4141 gcgtgttgtt cagacatgtt ggttcctcat aaccctgctt tcaaacttgc ttcgataaat
     4201 tgatccaggc tgccgtccag cacggcctgc gtgttgcgag tttctacccc ggtacgcaga
     4261 tctttaatgc gggagtcatc aaggacataa gaacgaatct ggctgcccca gccgatgtca
     4321 gatttgttat cttccatcgc ctgtttctcg gcatttttct tctgcatctc cagttcataa
     4381 agcttcgctt tcatctgctt catggcctga tctttgttct tgtgctggga acggtcgttc
     4441 tggcactggg tcacgatccc ggtcgggatg tgggtaatac gcaccgcaga ttcggtacgg
     4501 ttaacgtgct gaccgcccgc gccggacgcg cgataaacgt caatgcgcag atccgccggg
     4561 ttgatttcga tatcaatatc atcatcaact tccggataaa caaacgcaga gctgaacgac
     4621 gtgtggcgac gaccgccgga gtcaaacggg ctcttacgca ccaggcggtg aacgccagtt
     4681 tctgtacgca gccagccgta agcgtaatcg ccggagattt tgatcgtcac ggatttaata
     4741 cccgccactt caccttccga ctcttcgatg atttcagttt tgaaaccacg cgattctgcc
     4801 cagcgcagat acatacgctc aagcatgctc gcccagtcct gtgcttccgt accgccagac
     4861 cccgcctgaa tatcgaggta gcagtcggcg ctgtcatatt cgccagagaa catacggcgg
     4921 aactcaagct gcgccagttt ttcttccagg gcgtcgagtt cagcaacggc ttcgttaaag
     4981 gtttcttcgt cgtcagcttc tacagccagt tccagcagac cagaaacatc ttccagcccc
     5041 tgtttcattt ggtcgagggt gtcgacaacg gcttcgaggg aggaacgctc tttacccagc
     5101 gcctgtgcgc gttcgggttc attccagaca tccggctgtt ccagctcggc gtttacttct
     5161 tccagacgct ctttcttggc gtcgtagtca aagatacccc ctaagaacgt cggagcgttc
     5221 cgtgaggtcc tgaatgcgat tatttaccgg attaatttca aacatggtct gatttctttt
     5281 attgagctag tcaaaatgcg gtgataagag cgggattgta cccaatccac gctctttttt
     5341 atagagaaga tgacgctaaa ttggccagat attgtcgatg ataatttgca ggctgcggtt
     5401 gccgcgaaac tcgttgatat cgagcttgta agccagttgc acttcgcgca cgccgttatc
     5461 cggccagagg gcggtatcga cattaaaagc aataccatcc agcagcggac cgccgccgac
     5521 cggttcgacc atgactttca aatgacgttc gcccaccagc cgctgttgca gcagccggaa
     5581 atgaccgtca aacagcggct ccgggaacat ctgcccccac gggccagcat cgcgcagcag
     5641 ctgcgccact tccatggtca tttcggccgg gcttaacgga ccgtctgaca ccacttcgcc
     5701 ttgcaatagc gaagggtcca gccactcagt aaccagttcg ccaaaccgtt gttgaaagag
     5761 ttcgaattta tccgcttcca gcgacaaacc cgccgccatc gcatgaccgc caaacttcag
     5821 catcatgcca gggtagagtg tgtctaatcg ctccagcgca tcacgcatat gcagcccctg
     5881 aatggagcga ccagaacctt tcagcgtacc atcacccgcg ggcgcaaagg cgataaccgg
     5941 acggtgaaaa cgctctttaa tacgggaagc cagaataccg acaaccccct gatgccattc
     6001 ggggtgatac attgccagcc cgccgggtag cgtgtcacgg ctgcgctcca gtttctcgca
     6061 cagggtcagg gcttcaacct gcattccttg ttcgatctct tttcgcgtct ggtttagcgc
     6121 atcgagttca tttgccagca cgcgcgcttc gccgatgttg tcgcacagca agagcgccac
     6181 accgacggac atatcgtcca gtcgtccggc agcattgaga cgtggcccca gcgcaaaacc
     6241 taaatcgctg gcggcgagtt tttgtgcatc acggtttgcc acttcaagca gcgctttaat
     6301 ccccggacgg cactttccgg cacggatgcg actcatcccc tgccaggtca gaatgcgatt
     6361 attagcgtcc agcggtacga cgtccgccac tgtccccagc gctaccagat ccagcagttc
     6421 tgccaggtta ggaattgcga tgccgcgctc atcaaaccag ccctgatcgc gcaaaaaggt
     6481 gcgcagcgcc agcatcagat aaaacgccac acccacgcct gccagtgatt tcgacgggaa
     6541 attacagtcg cgcaagttag ggttaatgat cgcttccgcc gcgggtaatg tttcgcccgg
     6601 caaatggtga tcggtaacaa taaccggaat gcccaacgag cgagcgtgtt caacccccgc
     6661 atgggaggaa ataccgttat ccaccgtgac aattaactgc gcaccacggg catgggcctg
     6721 atcgaccact tccgggctta agccgtaacc gtcttcgaaa cggtttggaa ccaggtagtc
     6781 gatattgctg caaccaagcg agcgcatcgc cagcacgctt agagccgtgc tggtggcacc
     6841 gtcggcgtcg aaatcaccga ccacaataat ccgcgttcct tcgcgaaagg cgttgtaaag
     6901 gatctcaacg gccttttcga cgccgctcag ttgctgccag ggcagcatcc ctttaacact
     6961 gcgttccagt tcttgcgcac tgcgcacgcc ccggctggcg tataaacggc gcagcaaggg
     7021 aggcaattca gcgggcaagt ctgccgtttc atcgactgca cggcgacgaa gttgtatctg
     7081 ttgtttcacg cgaattattt accgctggtc attttttggt gttcgtcgag gaattctttc
     7141 atctctttcg gcggctggta acccggaaca agtgtgccat tgctcagcac aactgccgga
     7201 gtaccgctaa cgccaagctg gacgccaagt gcgtaatggt cggcaatatc cacgtcgcag
     7261 ctggctggtg cgacgctttt acctgccatc acatcatcaa acgctttgtt tttatctttc
     7321 gcacaccaga tagctttcat ttctttctct gcatcgctgt ccagcccctg gcgcgggaaa
     7381 gcaagataac gcacggtgat ccccagcgcg ttatagtctg ccatttgctc atgcagtttg
     7441 tggcagtaac cacaggtaat atcagtaaac acggtgatga cgtgtttttc ctgcagcgct
     7501 ttataaacga tcatctcttt ttcaagcgca ttcaactgct ttaacagcat cttattggtg
     7561 acattgaccg gagccgtgcc gctaacgtca tacattggcc cctgaatgat atgtttacca
     7621 tcatcggtga tgtacaacac gccgctgtta gtcagaactg tcttcatgcc agctacgggc
     7681 gcgggctgaa tatcgctgct tttgatgccc attttggcta acgtttgttg aattgccgcg
     7741 tcatcagcct gagcaaaacc tgaaaacgcc gctaacaaag taaacaacat aaaacctttc
     7801 ttcataaatc ttcccgttct tttcagacat cacgcccgcg ggtgatgctg ttgatgaagt
     7861 tgccgcagac gctcggtagc gacatgcgta taaatttgcg tggtggaaag atcgctgtgg
     7921 cccagtagca tctgcaccac gcgtaaatcc gcaccatgat ttaataaatg agtggcaaaa
     7981 gcgtgacgca acacatgcgg tgacagcttt tcgctgtcga tacccgccag cacggcataa
     8041 tgtttaatgc ggtgccagaa ggtctgtcgc gtcatctgct gcgcacgctg gctgggaaac
     8101 aacacgtcaa ttgacacacc attcagcagc cacggacgcc catgttccag ataggtttcc
     8161 agccagtaaa ccgcctcttc acctaacggt accaggcgct ctttgttgcc tttaccaatg
     8221 acccgcacta cgccctgacg caggctgata tcactcattg tcagcccgac cagttcagag
     8281 acacgcagcc cggtagcata caacacttca agcatggctt tatcgcgtag ctccagtggc
     8341 tgatcaatta atggcgcctg taataaacgt tcgacctgcg cttcgcttaa atcttttggc
     8401 aaacgctggg gcaatttcgg tgaagcgaga tgcgcactgg gatcgtcttc acgaaacttt
     8461 tcgcgataaa gatactggaa caatcggcgc actgcactca gcaaacgcgc tgagctggtg
     8521 gctttatacc cgccctccag ccgttctgcc agtaatgcct gcaaatcgtc actttgcgcc
     8581 gtcgccagcg tcaacccgcg gtgatgcaac cactccacca tcattgacag atcgcgacgg
     8641 taagcgttca acgtattttc agccagattt ttttccagcc acagagcatc aagaaactgc
     8701 tcgatgcgtg ccagatcctg tttcacttgc gccccttatg gtcactcatt tgatccatta
     8761 tgccttattg tgccgtgact aaagcgattc tgatacacta gctgcaaaag ccacagcaga
     8821 atcgagaagc ttacgttatg aatatgggtc ttttttacgg ttccagcacc tgttacaccg
     8881 aaatggcggc agaaaaaatc cgcgatatta tcggcccaga actggtgacc ttacataacc
     8941 tcaaggacga ctccccgaaa ttaatggagc agtacgatgt gctcattctg ggtatcccga
     9001 cctgggattt tggtgaaatc caggaagact gggaagccgt ctgggatcag ctcgacgacc
     9061 tgaaccttga aggtaaaatt gttgcgctgt atgggcttgg cgatcaactg ggatacggcg
     9121 agtggttcct cgatgcgctc ggtatgctgc atgacaaact ctcgaccaaa ggcgtgaagt
     9181 tcgtcggcta ctggccaacg gaaggatatg aatttaccag cccgaaaccg gtgattgctg
     9241 acgggcaact gttcgtgggt ctggcgctgg atgaaactaa ccagtatgac cttagcgacg
     9301 agcgtattca gagctggtgc gagcaaatcc tcaacgaaat ggcagagcat tacgcctgat
     9361 ctcactgacg gcttagcgca tatgctttgc cgtcatcttt atctttgcgt ctcttgttgc
     9421 aacaaaatcc gccgtaaatc ccgccattcg gcttcgtcca tgctgtcggc tgccagccat
     9481 aaatgttgcc gtttaccgcc atcagaacgt aaacgcagca tcatgccgct cttaatcatc
     9541 cacggtgctt tgacgatgct ccactcctgc ccttgccaac gcaaacgccc gtccatcaac
     9601 aagcgaattt ccccctggcg agcattaata cgccgctggc tgcgaacgca atcaaacacc
     9661 accagcgaaa gtaacaccat ccataacggg gtgtaactga gtggccaggg catgagtaaa
     9721 ataacagcgg caaccagccc atgaatcagc aaggaaagcc actgtgcgcg ccaggagacg
     9781 cgcaaatcag attgccacag gaccacgttc ccggttccgt gtctggatga gtcggaccat
     9841 catttccagt tctgcatcgg ccggtttacc gtgattcatc agccagttaa acaggtccgg
     9901 atcgtcacat tccagcagac gaataaagat gcgtttttcg tcatcgctta agctgtcgta
     9961 ctcatgttcg aaaaacggca tgattgaaat atcgagttcg cgcataccac ggcggcatgc
    10021 ccaatgaatg cgggctttgt tgttaatgtc catcttcttc ctgtctcacg aaaatccagt
    10081 accccgctat tgtaacgtgt ttttcgcctt cttttacggg aatatcagta aacaccatcg
    10141 cgatcacgaa attaattcac aacaattcaa tggcttcatt tttttggaag tcgcctcgca
    10201 gaaggcacag atcgcgtagt gaaagcactt gcattgcctc atagctcttt taccattagt
    10261 cattaatacg ccgttaagca actcaggact ctattatggc ttttacacct tttcctcccc
    10321 gtcagcctac ggcttctgcc cgtttaccgc tgacgctgat gacgcttgat gactgggcgc
    10381 ttgccaccat tactggcgcg gacagcgaaa aatatatgca gggtcaggtg acagcagatg
    10441 tcagccagat ggcagaagat cagcacctgc tcgccgccca ttgcgacgcc aaaggcaaaa
    10501 tgtggagcaa tttacgtctg ttccgcgacg gcgatggctt tgcgtggatt gaacggcgca
    10561 gcgtgcgtga accgcagctg actgaactga aaaaatatgc ggtgttctct aaagtgacca
    10621 tcgcgccaga cgacgagcat gtgctgcttg gtgttgccgg ttttcaggcg cgcgccgcgc
    10681 tggcaaatat ctttagtgaa ctgccttcga aagaaaaaca ggtagttaaa gaaggcgcga
    10741 ccactttgct atggtttgaa tacccggcag aacgtttcct gatcgtaacc gatgaagcta
    10801 ctgctaatat gctgaccgat aaactgcgcg gtgaagcgga actgaacaat agccaacagt
    10861 ggctggcatt aaacattgaa gcgggtttcc cggtgattga tgccgccaac agcgggcagt
    10921 ttatcccaca ggcgaccaat ctccaggcgc tgggcggtat cagctttaag aaaggctgtt
    10981 ataccggaca agagatggtg gcgcgagcaa aattccgtgg tgccaataaa cgtgcgctct
    11041 ggttgctggc aggtagcgcc agccgactgc cggaagctgg tgaagactta gagctgaaaa
    11101 tgggcgagaa ctggcgtcgt accggtacgg tgctggctgc ggtaaaactg gaagatggtc
    11161 aggtcgtggt acaggtcgtc atgaataacg atatggaacc ggatagcatc ttccgcgtac
    11221 gcgacgatgc gaatacattg catattgagc cgctgccgta ttcgctcgaa gagtaaatct
    11281 ctttatcgca tcaggcattt atcgcctgat gcgacgctga cgcgtcttat catgtctggg
    11341 atttgatgcc tttatcacaa cagggacgag gcccgtagat cggataaggc gttcacgccg
    11401 aatccggcag ttgtgctccg atgcctgatg cgacgctgac gcgtcttttc aggcctacaa
    11461 aggcataccc attacgcctg cccaatatac aaatagatcg ccagaaagtg gcacacactg
    11521 ccgccgagca cgaagccgtg ccagatggca tgattgtagg gaatgcgttt gcagacatag
    11581 aaaatcactc cgagcgaata aaccacgccg cctaccgcca ataaggtaac gctgcccgcc
    11641 gcgagcttaa ctgccatttc ataaattacc accagcgaca gccagcccat cgccagatag
    11701 gtcaccagag ataaaatttt gaatcggtgc gcgatggtca gtttaaacag aatacccagc
    11761 aatgccaggc tccagataac aatcatcaac ccgcgcgcta acggagaatc cagccccacc
    11821 agcaaaaacg gcgtgtaggt tccggcaatc aacaggtaaa tagcgcaatg gtcaaatttc
    11881 ttcagccaca tttttgcccg ttgatgagga atggcgtgat agagcgtcga agcgaggaac
    11941 agcaggatca tactgccgcc atagaggctg taactggtta tcgctgtggc gctggcattg
    12001 agatccaccg cctgaaccag tagcaacacc agcccaacga taccaaacac caacccaatg
    12061 ccgtgactga cgctgttggc aatttcctct gccagcgaat atccctgctt aatgaggggc
    12121 ttctgaacca taacttactc cggagaaacg tacacgcaca tgtatacctc tccagcgtaa
    12181 ctgagaatag ttccagtgaa cacctgttag ctaaaataaa ttctgatttg agactaatct
    12241 cctaaaaatc atgaaattaa atgcgaaatt tcaactaaca ggcgtgagtt caatttaaag
    12301 acatttaaat tcaatcacat aaaactgtgt ctgaccggga tagatgtcgg caatgacctt
    12361 tatcagttca gtcagggtca tattttcctg ctctgcatgt ttttccgtca gcgtatccag
    12421 cgttacggtt gaggttgcgg tgacttcaat cgtgcaaaaa taaccgtcat cttcaaaacg
    12481 cccgacacga agcacatcac ccgttttgaa gtgcgattca gactcgtcgc ggatggtgat
    12541 ggttttacgc ccagccagaa tgtcatcctg gaaacgttga aaaaaagtga tgtcgtttgg
    12601 ctgcatggta ctatttcctg taagaattga ctcatctgga gcctatgata gtgaaaaaac
    12661 tcaccttaca gaaagatttc ttatggggcg gcgcagttgc cgctcatcag gtcgaaggcg
    12721 gctggaacaa aggcggcaaa gggccgagca tttgtgacgt tctgaccggt ggcgcacacg
    12781 gcgtgccgcg cgaaatcacc aaagaagtct tgccaggcaa atactatcca aaccatgaag
    12841 ccgttgattt ttatggtcac tacaaggaag acatcaagct atttgccgaa atgggcttca
    12901 aatgttttcg gacttccata gcctggacgc gcatttttcc aaaaggcgat gaagctcagc
    12961 caaacgaaga agggctgaag ttctacgatg atatgttcga tgaactgctg aaatacaaca
    13021 tcgaaccggt gatcaccctc tcccactttg aaatgccgct gcatctggtg cagcaatacg
    13081 gtagctggac caaccgtaaa gtggttgatt tctttgtccg tttcgcggaa gtggtgtttg
    13141 aacgctataa gcataaagtc aaatactgga tgaccttcaa cgaaattaac aaccagcgta
    13201 actggcgtgc accgctgttc ggttactgct gctccggcgt ggtttatacc gagcatgaga
    13261 acccggaaga gacgatgtat caggtgctgc atcaccagtt tgtcgccagc gccctggcgg
    13321 tgaaagctgc gcgtcgcatt aacccggaga tgaaagtcgg ctgtatgctg gcgatggtgc
    13381 cgctctatcc ctactgctgt aacccggacg atgtgatgtt cgctcaggag tcgatgcgcg
    13441 aacgctacgt ctttaccgat gtgcagctac gcggctatta cccgtcctat gtgttgaacg
    13501 agtgggagcg tcgcggattt aacatcaaaa tggaagacgg cgatctcgat gtactgcgcg
    13561 aaggcacctg cgattatctt ggtttcagct attacatgac caacgcagtg aaggccgaag
    13621 gcggcaccgg cgatgcgatc tctggtttcg aaggcagcgt accgaacccg tatgttaaag
    13681 cgtctgactg gggctggcag attgatccag taggtctgcg ctatgccctt tgcgaactgt
    13741 atgagcgtta tcagaggccg ctgtttattg tcgaaaacgg ttttggcgct tacgacaaag
    13801 tggaagaaga tggcagcatc aacgacgact accgcattga ctacctgcgc gcccatatcg
    13861 aagagatgaa aaaagcggtg acttacgatg gcgtggatct gatgggctac acaccgtggg
    13921 gctgcatcga ctgcgtatcg ttcaccaccg ggcagtacag caaacgctac ggctttatct
    13981 atgtgaataa acatgacgac ggtactggcg atatgtcgcg ttcacgtaag aagagcttta
    14041 actggtacaa agaggtgatt accagcaacg gcgagaagct ttaagtcgat gaagtaccgg
    14101 atgcaatact tgttgcatcc ggtcagacaa cttccctgtt ttatttcccg cccgccaaat
    14161 cgataaaact tcccgtgacg taagaggctt tatcacttag tagccagaca atggcctgcg
    14221 cgacctcttc tgcctgtcca ccacgctgca tggggatgtt cgacttaacg cgatcgacgc
    14281 gtccaggctc gccgccgctg gcgtgcattt cggtaaaaat aaaccctggc cgcacgcagt
    14341 taacgcggac cccctgcgcg gcgacttcca gcgatagtcc ggtggttaac gtatcaatcg
    14401 cccctttcga tgccgcgtaa tcaacatatt cccctggcga acccaaccgt gaggccaccg
    14461 aaaagacatt gacgatagcg ccgccactgc caccattttt aagcgccatg cgttttaccg
    14521 cctcgcggca gcagagaaaa tatcccgtca tgttggtgga aagtactcgg ttgattcgct
    14581 ctgcggtaag gttttcaacg gtgcactggg taaacaagat cccggcgtta ttgaccagcg
    14641 ctgctagcgg ttcatcgtgc tgatcgattg ctgtaaacat tgcgatgacc tgattttcgt
    14701 cgctgatatc cgcctggagc acgaatgcct tgccaccggc ttgcgtgatt aagttcacca
    14761 cttcctgcgc cgcatggagg ttttgttgat aattaaccgc caccgtatac tcttctttcg
    14821 ccaacagtaa tgcagttgcc cgcccgatgc cgcgactgcc atcggtcaca agtgctatag
    14881 ccatttgttt ttccgagaaa aattaattcg ttgacgtatc tttgagattg ctaacaaact
    14941 ggctcaatct tcaatgttgg cacgcttttc ccctcgccct ttcagggaga gggccggggt
    15001 gagggtaaat attcgcgcca gtgccggcct gttcccctca ccctaaccct ctccccaaag
    15061 gggcgagggg actgtctgag cacttttgta ctttgtcatc tgactaaaaa ggcgccgaag
    15121 cgcctttaga aaatagtcga atcagtgaat tactggtatt cgctaatcgg tacgcaggag
    15181 cagaacaggt tacggtcgcc gtaaacatca tccagacgtt tcaccgtcgg ccagtatttg
    15241 tctgccacgc ctgccgggaa taccgcaact tcacggctgt acggatgcgc ccactcggcg
    15301 accagttcgc tctgaatgtg cggcgcgttc accagcgggt tatcttccag cggccagaca
    15361 gcggctttca cctggtcaat ttctgcgcgg atagccagca tcgcgtcgat aaagcgatcc
    15421 agttccactt tgctttcaga ttcagtcggt tcaaccatca gcgtacccgc caccgggaac
    15481 gacatcgtcg gcgcgtggaa accgtagtcg atcaggcgct tggcaatatc cagctcgctg
    15541 atgccggttt cttctttcag cgggcgaata tcgagaatac attcgtgcgc cacgcgaccg
    15601 tcgcgaccgg tatacagcac cgggaaggca tcctgcaggc ggctggcaat atagttggcg
    15661 ttgaggattg ccacctggct tgcttttttc agcccttctg cgcccatcat gcggatgtac
    15721 atccagctga ttggcaggat agaggcgcta ccgaacggtg ccgcagaaac tgcgccctga
    15781 cgggttaaca tgccttcgat ttgcaccacg ctatgacccg gtacaaacgg tgccagatgc
    15841 gctttcacac cgatcggtcc catacccgga ccaccaccgc cgtgcggaat gcagaaagtt
    15901 ttatgcaggt tgaggtgcga aacgtccgcg ccaataaagc ccggcgaggt gatgccaacc
    15961 tgggcgttca tgttcgcgcc atcaaggtaa acctgaccgc cgaactgatg cacgacttca
    16021 cacacttcac ggatcgtttc ttcatacacg ccgtgggtag aaggataagt caccatgata
    16081 caggagaggt tatcgcccgc ctgttccgct ttcgcgcgca gatcagtcag atcgatgttg
    16141 ccgtttttat cacacgccac aaccaccacc tgcattcctg ccatatgtgc agaagcgggg
    16201 ttagttccgt gcgcagaagc cgggatcagg cagatatcgc gatgcccttc gttgcggctt
    16261 tcatgataat gacgaatcgc cagcaggccc gcgtattcgc cctgtgcgcc ggagttcggc
    16321 tgcatacaaa cggcgtcgta accggtcagt ttcaccagcc agtcagccag ctgcgcaatc
    16381 atctgctgat aaccttcagc ctgttccggc gggcaaaacg ggtgcagttc ggcaaattcc
    16441 ggccaggtga ttgggatcat ctcggcggcg gcgttcagtt tcatggtgca ggaacccagc
    16501 gggatcatcg cctgattcag cgccagatct ttacgctcca gcgagtgcat atagcgcatc
    16561 atttcggttt cgctgtggta gcgattaaac accggatggg tgaggatttc gtcgtcgcgc
    16621 agcatcgcag gctggataga gcggctgtcg tgagccacgt ctttgtccag cgtgtcgatc
    16681 tccagcccgt ggttatcgcc cagcagcacg ctgaaaagct gcattacgtt ttcacgcgtg
    16741 gtggtttcat caagggtgat ccccacggcg ttcaggatat cgctacgcag gttgatttca
    16801 gccgcttcgg cacgcgtcag tacgcccgct ttgtcggcca cttccacaca caaggtgtcg
    16861 aaatagtgcg catggcgcag tttcagacct ttttgttgca ggcccgccgc cagaatatcg
    16921 gtcagacggt gaatgcggtt agcgatacgt ttcaggccaa ccgggccgtg ataaacggca
    16981 tacaggctgg cgatgtttgc cagcagtacc tgggaagtac aaatgttgga gttcgctttc
    17041 tcacgacgaa tatgttgctc gcgagtctgc atcgccatgc gcagcgcggt attgccagct
    17101 gcatctttcg atacaccgat aatacggccc ggcattgagc gtttgtattc atctttcgcc
    17161 gcaaagaatg ccgcgtgtgg gccaccgtag cccatcggca cgccgaagcg ttgcgccgaa
    17221 ccaaaaacaa tatccgcgcc ctgtttaccc ggcgcagtta acagcaccag cgccataata
    17281 tcggcggcaa cgctgaccac aattttgcgt gatttcagtt cgctaataag cgcagtgtag
    17341 tcgtgaattt caccggtagt gcctacctgc tgtaacagca cgccgaagac gtcctgatgg
    17401 tcgagcactt tttgcgcgtc atcgacaatc acttcaaaac caaaggtttc ggcacgagta
    17461 cggaccacat ccagcgtttg cggatgcaca tcggaagcca cgaagaagcg gttggcattt
    17521 ttcagtttgc tgacgcgttt cgccatcgcc attgcttcgg cggcagcggt ggcctcgtcc
    17581 agaagagaag cagaggccat atccagtcca gtcaaatcca gcgttacctg ctggaagttg
    17641 agcagtgctt caaggcggcc ctgggagact tcaggttgat acggagtgta cgcggtatac
    17701 cagcccggat tttccagcat gttacgcagg ataaccggcg gtagctgcac ggcggtgtaa
    17761 cccatgccga tgtaagacgt gaagcgttta ttgcgactgg caatagcctt gagttctgcc
    17821 agtgcggcgt attcggtcgc cggtgcgcca acctgcggcg gggtcgcaag ctgaatatct
    17881 ttcggtacaa tctggccggt cagcgcgttt aacgattgtg cgccaacggc attcagcatt
    17941 tcttgctgtt gcgcggcgtc cggtccgata tggcgttcaa taaaagcgcc gctgttttca
    18001 agctggctta acgtctgtgt catgagcgat ggttcctgaa acgtgcagtg aattgtgaac
    18061 ctctctcctt acgaagagag tgagggtgag gcataaattt tcctcaccct gatcctctcc
    18121 cgcagaagag gaataaagcc gttactcgtc ttctaacaat gcttcgtatg cggtcgcatc
    18181 cagcagtgat tccagttcgc tttcatcgct ggctttgatt ttaaagatcc agccacctgc
    18241 atacggttcg ctgttcacca gttccgggga atcgctcagt gcgtcgttta ccgccacgat
    18301 ttcaccgctt actggcgcat aaatgtctga cgccgctttt actgattcgg caaccgcgca
    18361 gtcatcgccc gcgctaaccg ttgcgcccac ttccggcagg tcaacaaaca ccatatcgcc
    18421 taacagctcc tgagcatgtt cggtaatacc aacggtgtaa gtgccgtcgg cttctttacg
    18481 cagccattcg tgttctttgc tgtatttcag ttctgctggt acgttgctca tcaatcaatc
    18541 tccaaaaaag taaatcacgc gacggctttg ccgttacgca caaaaacagg ttttgtcact
    18601 ttaaccgaca tttcacggtt gcgaatttgc acaatcgccg tttcgccaat accttccagc
    18661 acgcgcgcca gcgcaatgct gtaacccagc gtcggggaga aagtaccgct ggtgataatg
    18721 ccttcatgct ggttgccctg cgcatcggta aagcgtaccg gcagttcatt acgcagcacg
    18781 cctttttcgg tcatcaccag accaaccagt ttttctgtac catgctcacg ctgcacttcc
    18841 agggcttcac gaccgataaa gtcacgatct gccggttccc aggcgatagt ccagcccatg
    18901 ttggcggcta aaggagaaat agtttcgtcc atctcctgac cataaagatt catgcccgct
    18961 tccagacgca gcgtgtcacg cgcgcccaag ccacatggct taacacccgc ttccaccagc
    19021 gcacgccaga aatcggccgc tttttcattg ggcagcgcaa tttcatagcc cgcttcaccg
    19081 gtataaccag tggtggcaat aaacagatcg cccgcctgca cgccaaagaa cggtttcatc
    19141 ccttccaccg cctgacgctg ggcgtcatta aacagtgtgg cagcttttgc ctgcgcattc
    19201 ggcccttgca cggcaatcat ggaaaggtca tcacgaacgg taatttcgat gccgaaaggt
    19261 tcagcgtgtt gggtaatcca ggagaggtct ttttcgcggg tggcggagtt aacaacgagg
    19321 cggaagaaat cttcagtaaa gtagtagacg atgaggtcat ctatcacacc gccagaggca
    19381 ttcaacatcc ccgagtaaag ggctttgccg cttttggtga gcttcgccac atcgttcgcc
    19441 agcagataac gcagaaactc ccgggtgcgg ctgccgcgga gatcgacgat ggtcatatgt
    19501 gacacatcaa acattccggc atcggtacgt accgcatgat gttcgtcgat ttgcgaaccg
    19561 taatgcagcg gcatcatcca gccgtggaaa tccaccatgc gagcgccgca aagcgtgtgt
    19621 tgttcgtaca aaggagtctg ttgtgccatc ttgtcctcat tgaataagcg gggctgacaa
    19681 cttttcatgg tgaaattatc accacgaaac ccagcatcgg agccactccc ggtccccaac
    19741 gcaatcgttc tcttttgcct gaacttacca ccgaaacaga ctgttaacca taaggtaaaa
    19801 ttgatcatca cattagctta tggttaaaaa acgccaaaat cgcgacagaa taaaaaacca
    19861 aaaaacacac cagtttctat ataaagatga tgccattaga aagctaattt gaataaggca
    19921 atattaagag ctaaaaaaat gtcaaaaaac actaaatcaa aaaataatga cattagaaaa
    19981 tataatgcga aaacggaggt gaaattagtt tatttcaaat gaggaaaatc tcccggcgaa
    20041 aaaaccggga gatgaaagtg tgatgggtat caaataaaca gcagaagaga aatttttaac
    20101 gcagccattc aggcaaatcg tttaatccca ttgcctggcg gataagttgt ggtttaacgc
    20161 caggaagcgt gtcggccagt ttcaaaccaa tatcacgcag cagttttttc gccggattgg
    20221 taccggaaaa cagatcgcgg aatccctgca taccagccag catcagcgcc gcactgtgct
    20281 tgcggctacg ctcataccgg cgcaggtaaa tgtactgccc gatgtctttc ccctgacgat
    20341 gcaaccgttt cagttcagca atcagctctg cagcatccat aaagccaaga tttactccct
    20401 gccccgccag cgggtgaatg gtatgcgcgg cgtcgcccac cagcaccaga cggtgcgcgg
    20461 caaactggcg cgcataacgc cccgtcagtg ggaacacctg acgcgcgctc tcaaccttgc
    20521 ataagcccag gcgattatca aaagcgatat ttaacgcgcg attaaattca tcttcacttg
    20581 cctgctgcat ccgctgcgct tcctctggcg acagtgacca gacaattgag caaagatgcg
    20641 gatcgctaag cggtaaaaag gccagaatgc cttcgccatg gaaaacctgc cgcgccaccg
    20701 catcatgcgg ttcttccgtg cgaatggtcg ctaccagcgc gtgatgctga taatcccaga
    20761 aagtcagcgg aatatctgct ttgttgcgca accaggaatt agcaccgtcc gcgccaatca
    20821 ccagacgcgc cgttaacatg ctgccatctt tcagcgtcag gaaagtttca ttttctcccc
    20881 aggcgacctg ctgtaattct gcgggggcca acagagtgat atctgacgac tgctgcgctt
    20941 tgttccacag cgcgtagtga atcactgagt tttcaacgat atgcccaaga tggctatagc
    21001 ccatgctttg atcgtcaaac gaaatgtgac caaaactgtc tttgtcccac acttccatac
    21061 cgtgataaca actggcccta cgagagagaa tgtcctgcca gacgccaaga cgggtgagta
    21121 atttttcgct ggcggcattg atagccgaaa cgcgcagttg tggtggtgca tccgccgcca
    21181 gaggttccgg tacgcgctgc tccagtacgg caacgcgtaa gccgctcccc tgtaagccgc
    21241 aggcaaccgc cagccccacc atgccgccac caacaatggc tacatcaaca ctttgcattg
    21301 tttattcctt aaaaccgcct tcaacgcgcc acccaaccga gggtgcgctg cgccagcaca
    21361 tcgcgtgccg gggtgaataa ttccatcgtc atcagtccga tgttgcgccc gacaaccagc
    21421 ggtgcccaac ggttggcaaa aagatgtacc aggctgtccg tgacgccgat ggttgcttcg
    21481 cgatcgctct gtcgacgctg ctgatagcgg cacaatatgc cgtaatcacc gatgtcttct
    21541 ccgcgctcat gcgcctgagt cagggtttcc gcaaggctca tgacatctcg cataccgagg
    21601 ttaaaccctt gcccggcaat cgggtgcaga gtttgcgccg cattgcccac cagcacggta
    21661 cgatgggtaa tagctctggc ggcgcgggtt aacgccagcg gataagcact gcgtttaccg
    21721 gcgtgggtaa tttttccaag tcgccagcca aaggccgact ggagttcacg gcaaaacttc
    21781 tcgtcgctcc acgacagcac ctcttcgcgc cgttccagtg gatgacacca gaccagcgaa
    21841 cagcgtccgt cagacatcgg caacatcgcc agcgggccat gttgcgtgaa gcgttcaaaa
    21901 gcgcggcctt cgtgcgcaac ggaagtcgca acgttggcaa tcacggccag ttgttcgtaa
    21961 ggctcctgct gccagtcaac gccgcacgcg gtggctaacg ctgaatgggt gccatcagct
    22021 gctaccagca cgcggcccgt cagcgtctcg ccactctcca gcgtcacttc aacgtgactc
    22081 tgagtacggg caacgttagc cacgcgatca gggcaatgca gcgttacgcc aggtgcttta
    22141 cgcagcaatg caaacagccg ttgcccgaca ttgtgcaatt cgacaacctg tcccagcgcc
    22201 gccagttggt aatcttctgc ggcgagggtg acaaatccag cgtgaccacg atcgctgaca
    22261 tgcacggtag tgatggcggt tgcgtaatcc gccagagatt gccagacgcc gatgcgcgcc
    22321 agttgctgac aggtccccgc agccagcgca atagctcgtc catcaaagcc cggatgagca
    22381 tgtgactctg gcgcggtcgc ttcaatcaaa tgtaccggca gcgccccgtg acttaaccgg
    22441 gaaatagcca gcgccagcgt cgcgcccgcc atgccgccac cgacgatgat tacgctcatt
    22501 gctttctcgc agctgccatc aacgcttcga tttcttccgg ctttttcacc acgctggcgg
    22561 tgaggttttc gttgccggtt tcggtaatca caatgtcgtc ttcaatacga atgccgatac
    22621 cgcgatattg ttctggcaca tccgcatccg gcgcgatata cagaccaggc tctacggtca
    22681 gtaccatccc cggttccaga atgcgcgagc gatcctgacc ataaacgcca acgtcatgga
    22741 catccagtcc taaccagtgg ctaaggccat gcataaagaa aggacgatgg gcgttctgag
    22801 cgatcagttc atcaacatca cctttcagga tgccgagttt taccaggccg ctaaccatga
    22861 tgcgcaccac ttcaccagtg acttccagaa tggaagttcc cggacgatac aggcgcaggc
    22921 tggtttcgag agactccagc acaatgtcgt agatttcacg ctgggcctgg gtgaatttgc
    22981 cgttgaccgg gaaggtgcgg gtaatatcgc ccgcgtaacc tttgtattca caacccgcgt
    23041 caatcaacac caggtcaccg tcgcgcagtt cactctcgtt ttcggtgtag tgcagaatgc
    23101 agccgttttc accgctgccg acaatggtgt tataggacgg atagcgcgca ccgtggcggt
    23161 taaattcgtg gtgaatttcg ccttccagat ggtactcgaa cattcccgga cggcattttt
    23221 ccatcgcccg tgtatgtgcc atggcggtga tttctcccgc gcggcggagt acggcaatct
    23281 cttctggcga tttgaacagg cgcatttcat gaacaacagg acgccagtcg atcatcgttg
    23341 ccggtgcggt gagattttgc cgcgaacctt tacgcagttt ttccagcgca ctgttcatga
    23401 tttcatcagc atatgcatat tcgccctggg catggtaaac cacatccagg ccgttaagta
    23461 gttgataaag ttgctgattg atttcgctga atgccagtgc gcggtcaacg cccagtttct
    23521 ctggcgcggc atcctggcct aagcgacggc caaaccagat ctccgccgtc aggtcgcgaa
    23581 cgcggttaaa cagaacgctg tggttatgag tgtcatcgct tttaatcagc accagcaccg
    23641 cttccaattc gttaaagccg gtgaagtacc agaagtcact gttctgacga taggggtatt
    23701 cgctgtcggc gctacgtgtt acttctggtg cagcaaaaat cagcgcggcg ctgccgggtt
    23761 gcatttgctc caccagggcc tgacggcgac gctgaaactc ttgccgggat atctcactca
    23821 taacactctc cttacgtttt ttgtttttag tgtagagtcg gtttttgtac ttctggcgcg
    23881 gtcggttgcg gatgagtaaa ggtgtcgtgg cataacagcg cggcaacacg aacgtattcg
    23941 atgatctctt caagcgacat ttcaagctct tcctgatctt cgtcttcgtc gtaacccagt
    24001 tgcgcaatgt tacgcagatc gtcgatagct tcaccggttt cgccggtcac tttatccagc
    24061 ttcggttgcg taacgccaag accaagcagg aagtgattga cccaacctgc caatgcatca
    24121 gcccgatcga aaacgctgac atcatcgcca tcaggcagat aaagctgaaa aaggaagccg
    24181 tcatcctgca gggcatcgct ggtggcagag tgcattttac gcagtgcctg tgccagctca
    24241 tgaccgaaag ccatgccttc gttcgtcagg tcgtgaagta gcggcagcca tgagctgtca
    24301 tcgttaccgc cacatatcat cccactgatt aaaccatgca tctcggcagg ggtcagcccc
    24361 gtcccttgtt ggttcagata ctggttcatt tcgttgtaac caggcatttc gttctgtata
    24421 gacataagca ttcgtcgtca aagggaggaa tattcatgat atgctaccac tttgggccct
    24481 ggtggaccag aaaagggctt gtctcttctc atcagggtag ctatagtgtc gccccttcgc
    24541 agaccatggg tctaaagacg aaggcagcgc agtcaatcag caggaaggtg gcatgtctgc
    24601 acaacccgtc gatatccaaa tttttggccg ttcactgcgt gtgaactgcc cgcctgacca
    24661 aagggatgcg ttgaatcagg cagcggacga tctgaaccaa cggttgcaag atctgaaaga
    24721 acgcactaga gtcacaaata ctgaacagtt ggtcttcatt gccgcattga atatcagcta
    24781 tgagttagcg caagaaaaag cgaagactcg tgactacgcg gcaagtatgg aacagcgtat
    24841 tcggatgctg cagcagacca tagaacaagc gttacttgaa caaggtcgca tcaccgaaaa
    24901 aactaaccaa aactttgaat gacacttttc ggtttactgt ggtagagtaa ccgtgaagac
    24961 aaaatttctc tgagatgttc gcaagcgggc cagtcccctg agccgatatt tcataccaca
    25021 agaatgtggc gctccgcggt tggtgagcat gctcggtccg tccgagaagc cttaaaactg
    25081 cgacgacaca ttcaccttga accaagggtt caagggttac agcctgcggc ggcatctcgg
    25141 agattccctt cttatctggc accagccatg acgcaactac cagaactccc actgacatta
    25201 tcccgacaag aaatccgcaa aatgattcgg caacgtcgtc gtgcgttaac gccggaacaa
    25261 cagcaggaaa tgggtcaaca agccgctacc cggatgatga cttatccccc ggtggtgatg
    25321 gcgcatacgg tcgctgtatt cctctctttt gatggtgaac tcgacaccca gccgctaata
    25381 gaacaactct ggcgcgccgg taagcgcgta tatcttccag ttttgcatcc ctttagtgcc
    25441 ggtaatttgc tgttcctgaa ttaccatccg caaagcgaac tggtgatgaa caggttgaag
    25501 atccatgagc caaaactgga tgtgcgtgac gtgctacccc tttcccgatt agacgtgctg
    25561 atcacaccgc tggtcgcctt tgatgagtac ggtcagcgcc tgggaatggg cggtggtttt
    25621 tatgatcgga ccttacaaaa ctggcagcac tataaaacgc aaccggtggg ttatgcgcat
    25681 gattgtcagt tggtggaaaa actccccgtt gaagagtggg atatccctct tcctgcggtg
    25741 gttacaccgt cgaaagtctg ggagtggtaa gggcgataca cctgcatcgc cctgattgac
    25801 atcgttgatt ctttaaccta atttagtgag taagggtaag ggaggattgc tcctcccctg
    25861 tgactgactg ttaataagct gggaaactta tgagtaacag tacaatcagt atgatggcaa
    25921 gtcgcatcat aacccttctc cttcaagccc tcgcttcggt gagggcttta ccgttacagc
    25981 cccatgctgc ccagctatcg taagctctct ttaaataacc acaacgcatt gatctacctt
    26041 taaatctttt cttcgagcgt gctcgaaaaa cccatgcaaa acagagataa tttttgggaa
    26101 aaaggatcta ttacggcagt aaacgtgggc gatgaagtgg ctatcaaaaa gttcggttca
    26161 gggcgatgca ccccgcatcg ccctgattga catcgttgat tctttgacct aatttagtga
    26221 gtaagggtaa gagaggatga ctcctcccct gagactgact gttaataagc tccgaaactt
    26281 atcagtaaca gtacaatcag tatgatgaca agtcgcatca taacccttct cctttaagcc
    26341 ctcgcttcgg tgagggcttt accgttacag ccccatgctg tcccgccatc gtaaatcccc
    26401 attaaataaa cacaacgcat tgatctgagt ttgatttatt ttctggagca gactcgcaaa
    26461 gtagaatgcg caacgcggca acggtgtgga gaagggataa aaaaacgggc aagtcagtga
    26521 cctgcccgtt aattttcaga gaaggggaat tagtacagca gacgggcgcg aatggtaccc
    26581 ggaatagctt tcattgcctg cagcgctttt tcggcaacgt cttcgtcagc ttcaatatca
    26641 ataactacat aacccatctg ggcggaagtt tgtagatatt gcgcggcgat gttgacgccc
    26701 tgctcggcga agattttgtt cagcgcagtt agcacgcccg gacggttttc gtggatgtgc
    26761 atcagacgac gaccaccatg cagtggcagc gagacttccg ggaagttcac cgcagagagc
    26821 gttgagccat tgtcagaata cttgatcaat ttacccgcaa cttccaggcc gatattctcc
    26881 tgcgcttcct gagtcgaacc gccaatgtgt ggcgtcagaa ggacgttgtc gaattcacac
    26941 agtggagagg taaatggatc gctattggtc gccggttccg tcgggaatac gtcgattgcc
    27001 gcccccgcca gatgtttgct cgccagcgca tcacacagcg ccggaatatc caccacagta
    27061 ccgcgcgagg cattaatcag cagcgagccg ggcttcatta gcgaaatctc tttcgcgccc
    27121 atcatatttt tggtggacgg attctctggt acatgcagac tcaccacatc gctcatattc
    27181 agcaggtcag aaagatgctg tacctgagtg gcgttgccca gcggcagttt gttttcaata
    27241 tcataaaagt aaacatacat tcccagcgat tcagccagaa tgcccaattg cgtaccaata
    27301 tgaccgtagc cgatgatacc cagctttttg ccgcgcgctt caaaagaacc cgccgccagt
    27361 ttgttccaca cgccacggtg cgctttagca ttggcttccg gcacgccgcg caatagcagc
    27421 agcagttcgc caatcaccag ctccgcaaca gagcgcgtat ttgagaacgg tgcgttaaat
    27481 accgggatcc cgcgctttgc cgccgcatcc agatcaacct ggtttgttcc gatacagaaa
    27541 cagccaatag cgaccagttt ttctgcggcg ttgatcacgt cttcagtcag atgggtacgg
    27601 gatcgcaggc cgatgaagtg ggcatcgcgg atggattctt ttaattgttc atcatccagc
    27661 gcgcctttgt gaaattcgat gttggtgtaa ccagctgcac gaaggctttc cagcgccttt
    27721 tggtgcacgc cttctaccag cagaaactta atcttgtctt tctccagcga tacctttgcc
    27781 atttacccaa tcctgtcttt tgaaatgttg tgtgcggatt tgcatccgcc tttcaacata
    27841 tcaaaaaata atattgcggc aatatgaacg tttgcgtcgc gatgttgagg aaatatcacg
    27901 caaaggtatt tcttggttga atatgctggt ttagaggatt tatttagagc aatcgacaat
    27961 agccaagtaa aaacgtgaca catgtcacca aatttaatga agagaatttt tttaacgggg
    28021 gaggttcccc cgtcagatca tttcacaatg gttttgacac cgtcaggtgt gccaatcagc
    28081 gcaacgtccg cgccacggtt agcaaacaag ccaacagtca ccacgccagg aatcgcattt
    28141 atggcgtttt ccatcgctat cgggtcaagg atttccatgc cgtggacgtc gaggatcacg
    28201 ttgccgttat cggtcaccac gccctgacgg tattccggac gaccgcccag tttcaccagc
    28261 tggcgcgcca ctgcactacg tgccatcggg ataacttcta ctggcagcgg gaatttgccc
    28321 agaatatcaa cctgcttgga agcgtcagca atacagataa atttttccgc aaccgaagca
    28381 atgatttttt cacgggtcag cgccgcgccg ccgcctttga tcatttgcat gtggccgttg
    28441 atttcatctg cgccatcaac gtagatgcca aggctgtcga cttcgttgag atcaaaaacg
    28501 tgaatgccga ggcttttcag tttttcagtg gaagcatctg aactggaaac ggccccttca
    28561 atctggcctt tcattgtacc gagcgcgtca ataaagtgtg cggcggtgga acctgtacct
    28621 acaccaacaa tggtgccggg ctgaacatac tgaagtgccg cccatcctac tgcttttttc
    28681 aattcatcct gcgtcatgat cgtttcgcct gtggtatgaa atttcacacg cattatatac
    28741 aaaaaaagcg attcagaccc cgttggcaag ccgcgtggtt aactcatcca taaaatatcg
    28801 cgcaatggcg ggcatcccct ttcgccccgc aaataaagca tacaacggtc tgggtatgcc
    28861 gctccacggt gcgaacaggc gcaccagttc cccccgttcg caagcccctg tttacaggca
    28921 aattgaggca ataacgccac cccattcaac acaaccaggg cacgaactcg ctgagcgaaa
    28981 tgcattagcg caaatccctg atcttttaag ctgcgcgttt aaccacggat acaaatgtta
    29041 cccgccgacg gcttcggtat atgcaacctg acacaaaatt gtgtcatagt gcaggaaaaa
    29101 gcatttacca ggagcagaca acagcaatga aacgcccgga ctacagaaca ttacaggcac
    29161 tggatgcggt gatacgtgaa cgaggatttg agcgcgcggc acaaaagctg tgcattacac
    29221 aatcagccgt ctcacagcgc attaagcaac tggaaaatat gttcgggcag ccgctgttgg
    29281 tgcgtaccgt accgccgcgc ccgacggaac aagggcaaaa actgctggca ctgctgcgcc
    29341 aggtggagtt gctggaagaa gagtggctgg gcgatgaaca aaccggttcg actccgctgc
    29401 tgctttcact ggcggtcaac gccgacagtc tggcgacgtg gttgcttccg gcactggctc
    29461 ctgtgttggc tgattcgcct atccgcctca acttgcaggt agaagatgaa acccgtactc
    29521 aggaacgtct gcgccgcggc gaagtggtcg gcgcggtgag tattcaacat caggcgctgc
    29581 cgagttgtct tgtcgataaa cttggtgcgc tcgactatct gttcgtcagc tcaaaaccct
    29641 ttgccgaaaa atatttccct aacggcgtaa cgcgttcggc attactgaaa gcacctgtgg
    29701 tcgcctttga ccatcttgac gatatgcacc aggccttttt gcagcaaaac ttcgatctgc
    29761 ctccaggcag cgtgccctgc catatcgtta attcttcaga agcgttcgtg caacttgctc
    29821 gccagggcac cacctgctgt atgatcccgc acctgcaaat cgagaaagaa ctagccagcg
    29881 gtgaactgat tgacttaacg ccagggctat ttcaacgccg gatgctctac tggcaccgct
    29941 ttgctcctga aagccgcatg atgcgtaaag tcactgatgc gttgctcgat tatggtcaca
    30001 aagtccttcg tcaggattaa tccatcaaat aatgcctgat agcacatatc aggcgttatc
    30061 ctcacttctt tttgtattcc ttgaatcaca tcacaaaata gacaaatctc aggcggcaaa
    30121 aaacgacgtc tgaatgcatt tttttgctgg cgacaaaccc acgtaaaaag ctcaccgtaa
    30181 gcgcaaatac cctcattttg attgcgtttt acggagcaaa taatgtctaa cgagcaggag
    30241 tggcaacagc ttgccaacaa ggaattgagc cgtcgggaga aaactgtcga ctcgctggtt
    30301 cagcaaaccg cggaagggat cgccatcaag cagctgtata ccgaagccga tctcgataat
    30361 ctggaggtga caggtaccct tcctggtttg ccgccctacg ttcgtggccc gcgtgccact
    30421 atgtataccg cccaaccgtg gaccatccgt cagtatgctg gtttttcaac agcaaaagag
    30481 tccaacgctt tttatcgccg taacctggcc gccgggcaaa aaggtctttc cgttgcgttt
    30541 gaccttgcca cccaccgtgg ctacgactcc gataacccac gcgtggcggg tgacgtcggc
    30601 aaagcgggcg tcgctatcga caccgtggaa gatatgaaag tcctgttcga ccagatcccg
    30661 ctggataaaa tgtcggtttc gatgaccatg aatggcgcag tgctaccagt actggcgttt
    30721 tatatcgtcg ccgcagaaga gcaaggtgtt acacctgata aactgaccgg caccattcaa
    30781 aacgatattc tcaaagagta cctctgccgc aacacctata tttacccacc aaaaccgtca
    30841 atgcgcatta tcgccgacat catcgcctgg tgttccggca acatgccgcg atttaatacc
    30901 atcagtatca gcggttacca catgggggaa gcgggtgcca actgcgtgca gcaggtagca
    30961 tttacgctcg ccgatgggat tgagtacatc aaagcagcaa tctctgccgg actgaaaatt
    31021 gatgacttcg ctcctcgcct gtcgttcttc ttcggtatcg gcatggatct gtttatgaac
    31081 gtcgccatgt tgcgtgcggc acgttattta tggagcgaag cggtcagtgg atttggcgca
    31141 caggatccga aatcactggc gctgcgtacc cactgccaga cctcaggctg gagcctgact
    31201 gaacaggatc cgtataacaa cgttatccgc accaccattg aagcactggc tgcgacgctg
    31261 ggcggtactc agtcactgca taccaacgcc tttgacgaag cgcttggttt gcctaccgat
    31321 ttctcagcac gcattgcccg caacacccag atcatcatcc aggaagaatc agaactctgc
    31381 cgcaccgtcg atccactggc cggatcctat tacattgagt cgctgaccga tcaaatcgtc
    31441 aaacaagcca gagctattat ccaacagatc gacgaagccg gtggcatggc gaaagcgatc
    31501 gaagcaggtc tgccaaaacg aatgatcgaa gaggcctcag cgcgcgaaca gtcgctgatc
    31561 gaccaaggca agcgtgtcat cgttggtgtc aacaagtaca aactggatca cgaagacgaa
    31621 accgatgtac ttgagatcga caacgtgatg gtgcgtaacg agcaaattgc ttcgctggaa
    31681 cgcattcgcg ccacccgtga tgatgccgcc gtaaccgccg cgttgaacgc cctgactcac
    31741 gccgcacagc ataacgaaaa cctgctggct gccgctgtta atgccgctcg cgttcgcgcc
    31801 accctgggtg aaatttccga tgcgctggaa gtcgctttcg accgttatct ggtgccaagc
    31861 cagtgtgtta ccggcgtgat tgcgcaaagc tatcatcagt ctgagaaatc ggcctccgag
    31921 ttcgatgcca ttgttgcgca aacggagcag ttccttgccg acaatggtcg tcgcccgcgc
    31981 attctgatcg ctaagatggg ccaggatgga cacgatcgcg gcgcgaaagt gatcgccagc
    32041 gcctattccg atctcggttt cgacgtagat ttaagcccga tgttctctac acctgaagag
    32101 atcgcccgcc tggccgtaga aaacgacgtt cacgtagtgg gcgcatcctc actggctgcc
    32161 gatcataaaa cgctgatccc ggaactggtc gaagcgctga aaaaatgggg acgcgaagat
    32221 atctgcgtgg tcgcgggtgg cgtcattccg ccgcaggatt acgccttcct gcaagagcgc
    32281 ggcgtggcgg cgatttatgg tccaggtaca cctatgctcg acagtgtgcg cgacgtactg
    32341 aatctgataa gccagcatca tgattaatga agccacgctg gcagaaagta ttcgccgctt
    32401 acgtcagggt gagcgtgcca cgctcgccca ggccatgacg ctggtggaaa gccgtcaccc
    32461 acgtcatcag gcgctaagta cacagctgct tgacgccatt atgccgttct gtggtaacgc
    32521 cctgcgactg ggcgttaccg gcacacccgg cgcggggaaa agtacctttc ttgaagcctt
    32581 tggcatgttg ttgattcgtg agggattaaa ggttgctgtt attgcggtcg atcccagcag
    32641 cccggtcact ggcggtagca ttctcgggga taaaacccgc atgaatgacc tggcgcgtgc
    32701 cgaggcggcg tttattcgcc cggtaccctc ctccggtcat ctgggcggtg ccagtcagcg
    32761 agcacgggaa ttaatgctgt tatgcgaagc ggcgggttat gacgtagtga ttgtcgaaac
    32821 ggttggcgtc gggcagtcgg aaacggaagt cgcccgcatg gtggactgtt ttatctcgtt
    32881 gcaaattgcc ggtggcggcg atgatctgca gggcattaaa aaagggctga tggaagtggc
    32941 tgatctgctc gttatcaaca aagacgatgg cgataaccat accaatgtcg ccattgcccg
    33001 gcatatgtac gagagtgccc tgcatattct gcgacgtaaa tacgacgaat ggcagccacg
    33061 ggttctgact tgtagcgcac tggaaaaacg tggaatcgat gagatctggc acgccatcat
    33121 cgacttcaaa accgcgctaa ctgccagtgg tcgtttacaa caagtgcggc aacaacaatc
    33181 ggtggaatgg ctgcgtaagc agaccgaaga agaagtactg aatcacctgt tcgcgaatga
    33241 agatttcgat cgctattacc gccagacgct tttagcggtc aaaaacaata cgctctcacc
    33301 gcgcaccggc ctgcggcagc tcagtgaatt tatccagacg caatattttg attaaaggaa
    33361 tttttatgtc ttatcagtat gtgaacgttg ccactatcaa caaagtggcg gtcattgagt
    33421 ttaactatgg acgaaaactt aatgccttaa gtaaagtctt tattgatgat cttatgcagg
    33481 cgttaagcga tctcaaccgg ccggaaattc gctgtatcat tttgcgggcg ccaagtggat
    33541 ccaaagtctt ctccgcaggt cacgatattc acgaactgcc gtctggcggt cgcgatccgc
    33601 tctcctatga tgatccattg cgtcaaatca cccgcatgat ccaaaaattc ccgaaaccga
    33661 tcatttcgat ggtggaaggt agtgtttggg gtggcgcatt tgaaatgatc atgagttccg
    33721 atctgatcat cgccgccagt acctcaacct tctcaatgat gcctgtaaac ctcggcgtcc
    33781 cgtataacct ggtcggcatt cacaacctga cccgcgacgc gggcttccac attgtcaaag
    33841 agctgatttt taccgcttcg ccaatcaccg cccagcgcgc gttggctgtc ggcatcctca
    33901 accatgttgt ggaagtggaa gaactggaag atttcacctt acaaatggcg caccacatct
    33961 ctgagaaagc gccgttagcc attgctgtta tcaaagaaga gctgcgtgta ctgggcgaag
    34021 cgcacaccat gaactccgat gaatttgaac gtattcaggg gatgcgccgc gcggtttatg
    34081 acagcgaaga ttaccaggaa gggatgaacg ctttcctcga aaaacgtaaa cctaatttcg
    34141 ttggtcatta atccctgcga acgaaggagt aaaaatggaa actcagtgga caaggatgac
    34201 cgccgatgaa gcggcagaaa ttatccagca taacgacatg gtggcattta gcggctttac
    34261 cccagcgggt tcgccgaaag ccttaccgac cgcgattgcc cgcagagcta acgaacagca
    34321 tgaggccaaa aagccgtatc aaattcgcct gctgacgggg gcgtcaatca gcgccgccgc
    34381 tgacgatgta ctttctgacg ccgatgctgt ttcctggcgt gcgccatatc aaacatcgtc
    34441 cggtttacgt aaaaagatca atcagggcgc ggtgagtttc gttgacctgc atttgagcga
    34501 agtggcgcaa atggtcaatt acggtttctt cggcgacatt gatgttgccg tcatcgaagc
    34561 atcggcactg gcaccggatg gtcgagtctg gttaaccagc gggatcggta atgcgccgac
    34621 atggctgctg cgggcgaaga aagtgatcat tgaacttaat cactatcacg atccgcgcgt
    34681 tgcagaactg gcggatattg tgattcctgg cgcaccaccg cggcgcaata gcgtgtcgat
    34741 cttccatgca atggatcgcg tcggtacccg ctatgtgcaa atcgatccga aaaagattgt
    34801 cgccgtcgtg gaaaccaact tgcccgacgc cggtaatatg ctggataagc aaaatcccat
    34861 gtgccagcag attgccgata acgtggtcac gttcttattg caggaaatgg cgcatgggcg
    34921 tattccgccg gaatttctgc cgctgcaaag tggcgtgggc aatatcaata atgcggtaat
    34981 ggcgcgtctg ggggaaaacc cggaaattcc tccgttcatg atgtattcgg aagtgctaca
    35041 ggaatcggtg gtgcatttac tggaaaccgg caaaatcagc ggggccagcg cctccagcct
    35101 gacaatctcg gccgattccc tgcgcaagat ttacgacaat atggattact ttgccagccg
    35161 cattgtgttg cgtccgcagg agatttccaa taacccggaa atcatccgtc gtctgggcgt
    35221 catcgctctg aacgtcggcc tggagtttga tatttacggg catgcaaatt caacgcacgt
    35281 agccggggtc gatctgatga acggcatcgg cggcagcggt gattttgaac gcaacgcgta
    35341 tctgtcgatc tttatggctc cgtcgattgc taaagaaggc aagatctcaa ccgtcgtgcc
    35401 aatgtgcagc catgttgatc acagcgaaca cagcgtcaaa gtgatcatca ccgaacaagg
    35461 gatcgccgat ctgcgcggtc tttccccgct tcaacgcgcc cgcactatca ttgataattg
    35521 tgcacatcct atgtatcggg attatctgca tcgctatctg gaaaatgcgc ccggcggaca
    35581 tattcaccac gatcttagcc acgtcttcga cttacaccgt aatttaattg caaccggctc
    35641 gatgctgggt taatggtttg tcccgtcacg atcttcagca atatgctgaa gcatcgtggc
    35701 ggtataacga tgatttctga ttgaataaag atattcctgc atatacatcg gcctgtgcgg
    35761 cgcatcaaaa tatttcagcg ttgccggatt caccagtcgc caggcaaaat gcgggatcac
    35821 cgtcagaaaa tgtccgcgct ccacggcgct gattttggcc ataaaactgt agggacgata
    35881 aataatggtg ggattgatcc cacaaggtcg catattcgca tcaatcattg cttcgaagtt
    35941 agcccggttt tgaaagcgca tttgtagcca gggcagctcc tgaagcagct cttgctcttg
    36001 acgctcttca taacggcgag aaacgagaaa gcccaatcgc agcggtggta attcactgat
    36061 agtcaggttt tccagttcct gaactcgtgc agatacatgc tgtggggaga taataaaatc
    36121 taactgacgg tcaaaaagat tatcgataac gccgttttcg ctgaactcaa ctggctgggc
    36181 ggtcacgcct tcatacttat ctccgagact aatcaactga tcgaaaataa ttgtcggata
    36241 agtattatct atgccaat
//
LOCUS       DAJVRF010000025        35779 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJVRF010000025 DAJVRF010000000
VERSION     DAJVRF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.025"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(1..581)
                     /locus_tag="OQN13_001151"
                     /pseudo
     CDS             complement(1..581)
                     /locus_tag="OQN13_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(810..1730)
                     /gene="malM"
                     /locus_tag="OQN13_001152"
     CDS             complement(810..1730)
                     /gene="malM"
                     /locus_tag="OQN13_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCS1614568.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVSYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="OQN13_001153"
     CDS             complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="OQN13_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCS1614569.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(3294..4409)
                     /gene="malK"
                     /locus_tag="OQN13_001154"
     CDS             complement(3294..4409)
                     /gene="malK"
                     /locus_tag="OQN13_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCS1614570.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4774..5964
                     /gene="malE"
                     /locus_tag="OQN13_001155"
     CDS             4774..5964
                     /gene="malE"
                     /locus_tag="OQN13_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCS1614571.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            6118..7662
                     /gene="malF"
                     /locus_tag="OQN13_001156"
     CDS             6118..7662
                     /gene="malF"
                     /locus_tag="OQN13_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCS1614572.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGADPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            7677..8567
                     /gene="malG"
                     /locus_tag="OQN13_001157"
     CDS             7677..8567
                     /gene="malG"
                     /locus_tag="OQN13_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCS1614573.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8939..10414
                     /gene="xylE"
                     /locus_tag="OQN13_001158"
     CDS             8939..10414
                     /gene="xylE"
                     /locus_tag="OQN13_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCS1614574.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VPLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="OQN13_001159"
     CDS             complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="OQN13_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCS1614575.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10994..11137)
                     /locus_tag="OQN13_001160"
     CDS             complement(10994..11137)
                     /locus_tag="OQN13_001160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614576.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            11138..11416
                     /locus_tag="OQN13_001161"
     CDS             11138..11416
                     /locus_tag="OQN13_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614577.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQSDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11463..13559)
                     /locus_tag="OQN13_001162"
     CDS             complement(11463..13559)
                     /locus_tag="OQN13_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCS1614578.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13559..14296)
                     /locus_tag="OQN13_001163"
     CDS             complement(13559..14296)
                     /locus_tag="OQN13_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCS1614579.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(14293..14931)
                     /locus_tag="OQN13_001164"
     CDS             complement(14293..14931)
                     /locus_tag="OQN13_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCS1614580.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(15045..15287)
                     /locus_tag="OQN13_001165"
     CDS             complement(15045..15287)
                     /locus_tag="OQN13_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614581.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="OQN13_001166"
     CDS             complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="OQN13_001166"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCS1614582.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            17923..19272
                     /gene="lysC"
                     /locus_tag="OQN13_001167"
     CDS             17923..19272
                     /gene="lysC"
                     /locus_tag="OQN13_001167"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCS1614583.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            19524..19796
                     /locus_tag="OQN13_001168"
     CDS             19524..19796
                     /locus_tag="OQN13_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCS1614584.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="OQN13_001169"
     CDS             complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="OQN13_001169"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCS1614585.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            21149..21986
                     /locus_tag="OQN13_001170"
                     /pseudo
     CDS             21149..21986
                     /locus_tag="OQN13_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            22056..22862
                     /locus_tag="OQN13_001171"
     CDS             22056..22862
                     /locus_tag="OQN13_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1614586.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            22872..23279
                     /locus_tag="OQN13_001172"
     CDS             22872..23279
                     /locus_tag="OQN13_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCS1614587.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            23279..23773
                     /locus_tag="OQN13_001173"
                     /pseudo
     CDS             23279..23773
                     /locus_tag="OQN13_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            23839..24636
                     /locus_tag="OQN13_001174"
     CDS             23839..24636
                     /locus_tag="OQN13_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCS1614588.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            24647..25471
                     /locus_tag="OQN13_001175"
     CDS             24647..25471
                     /locus_tag="OQN13_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCS1614589.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            25523..26751
                     /gene="sorE"
                     /locus_tag="OQN13_001176"
                     /pseudo
     CDS             25523..26751
                     /gene="sorE"
                     /locus_tag="OQN13_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            27134..27824
                     /gene="pepE"
                     /locus_tag="OQN13_001177"
                     /pseudo
     CDS             27134..27824
                     /gene="pepE"
                     /locus_tag="OQN13_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
     gene            complement(27915..29546)
                     /locus_tag="OQN13_001178"
     CDS             complement(27915..29546)
                     /locus_tag="OQN13_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCS1614590.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(29766..33449)
                     /gene="metH"
                     /locus_tag="OQN13_001179"
     CDS             complement(29766..33449)
                     /gene="metH"
                     /locus_tag="OQN13_001179"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCS1614591.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     VAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            33649..34473
                     /gene="iclR"
                     /locus_tag="OQN13_001180"
     CDS             33649..34473
                     /gene="iclR"
                     /locus_tag="OQN13_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCS1614592.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            34790..35779
                     /gene="espL4"
                     /locus_tag="OQN13_001181"
                     /pseudo
     CDS             34790..35779
                     /gene="espL4"
                     /locus_tag="OQN13_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
BASE COUNT         8598 a         9231 c         9102 g         8848 t
ORIGIN      
        1 actatgacca agttgactct gcgagaagtc aactgtatca agaatatttt ggctaaaatc
       61 aacacattcg ccactcgttt tgagaaaaat cacattgtgt aaacgtcgac atttaaatat
      121 gacattatca actgttgcct tagtaaaatt gcaattctca atttctttac attcgaaagt
      181 tacatgttgt agatttgatg ataaaaaaga caccttagaa ataacgtcac ctttaaaatg
      241 acaatttatt aaatcaaaat cagacaggtc gatatacccc tgcgcatctt tatccatcag
      301 aaaattacaa gcgcattcta ttttttcatt tctgtagaat tttacactta tagtatcatt
      361 actaattgag acttcacgta acatatttat tgcatgattt tttcgttccg ttgctttata
      421 tttaaaaata tatcactgat ataaataaag ttatctctgt ttaaacctgt caccccattg
      481 aaattacgta gtaaatcaag atctactata ttatttcctt gaaccacatc atgtttcatc
      541 aaagagttat tgccgccaga atttgtgata tgacctatca taaaacctct gtaatacaga
      601 aataaaataa cctggaaaag tagcctgagc gtccagacac taccggcgaa aggaaaagaa
      661 tctctgataa ggcattgaga taatggacat tcttagtgaa gaagaagtga agtactataa
      721 aaaatcgctc atatcccagg aaggaagcat aaaaaaaggt gcgccaggag acgcaccagt
      781 tgttgcaaaa tcagcactgt ggggcgtaat taccccttgc cttttacact gctgataaag
      841 gtggaacggg cagatgtcga tcccaggcgt tcagcttcat caagcagttt taacgcctta
      901 tcaacatcac ctttcgcgac agcgtttttg atcgcggtat taaaataact ttccgtgtcg
      961 ttgagcatcg gctcgctttt cttcaccggc accggagcag gtgcagccac agctggtgcc
     1021 gccgtgttgc ctaccgtaac cggagctgga gcggaagaac caaatagtgg tcctaccaac
     1081 acgctggagc tggagttcgt tttgactttc agtttcagta agccatcgct ggtatgacga
     1141 gcaaccggat cgggaatatc cgggatcgag ttaccgacgc ctttggcata ggctttagcc
     1201 gggtcgagca gttgggtcgt ctgctggaga tctttttccg tggtaaagac cagaacataa
     1261 agtttttgct gccccaacgc cggtgtcagg cgcataacgc cttccagccg atctgcactc
     1321 atcacgcctg gttcctggta ggtgaaataa ctgctgggga agaaggctga tggggtcatg
     1381 ttctgatcaa gaatcagcac gttcggcgca aaaacgctgg tttgtttgtt cacttcgctg
     1441 gtcagcgtca gggtcagttc gccaatgttt gccgggacgc tgtacgcagc aaccggacca
     1501 ctgatgccgg gaacgttcag ttgttggccg ccggtcgcca gttgggtggt ctgggtttta
     1561 gattgatcga ccggtgtcca ggtgagttgt tgcagcgcgg cagatggaat ggctggcgcg
     1621 tcgctggtgt tttgcggtac gtagctaaca tcggcaaggc taattccagg cgcgcttgcc
     1681 agtaaccctg ctgataaaca gagggcgatg agacttttat tcattttcat tgttttcacc
     1741 tcaaaatctg gagctcagcg gtagccaggc aatagcgcgc taaacccgat aatcagaggg
     1801 gctttcgccc ctttcagata atgacaacct gtttttatgc cggatgcggc gtaaacgcct
     1861 tatccggccc aggttttgct attaccacca gatttccatc tgggcaccga aggtccactc
     1921 gtcgctgtcg ccacgaccga agctgccgcc gttgaaatca gcaggaacgg ctttgccgta
     1981 gttcgggtta accttgctat cgccgttgta gtcgtaaccc catttctcat cccacttggc
     2041 gtaggttgcg aagacacgaa tagccgggcg tgaccagatg ctgtcgccag cctgccattg
     2101 ttgtgcgagg gtaattttgt actgattgtt cttgtcgccg gtgcgctggg attcgacgtt
     2161 gtcgtagccg atttccatca cggtgctcat gattggcgtc cacttgtaca tcgggcgaat
     2221 accgacggtc caccacttgg tgccgttgtc gttatcccag ttgatatcct ggtacatacc
     2281 cacgtacatc atgtcccagt tgtcgcccat ggagatcgca ccgtggtcga ggatacgcag
     2341 catgtgaccg ttgttgttga tattgtaggc aaatttttcg ttatcaaacg cgacgccaga
     2401 accctgcgac agacctttac cctgcgaggt catcgagtca gtagcgtact gaacaacaaa
     2461 cttgttaaag cccttcagga cactctgagt atgttcagca gtgaataacc agccgtcttt
     2521 cgatgcgcca tcaaccagac gatagttatc acgcaggttg gcacgaccgt agtcgacacc
     2581 cagttctaat gtgccgcccg ggttgatttc catctgcgct aaacgcacat cgaaaacgtc
     2641 gttcgcggtt tcgttggtat agtcataaat attgttgctg gcaaaagagg aagaaccacc
     2701 agcttcagag gagcgggttg ctgccagaga gagtttaccg aagccaacat cgatgttttc
     2761 cagaccggca ccaggaccag aaatatccca gtagtagaag tcgatcatat gaacgtcatg
     2821 acgttggtag aagcgcttac ctgcccagat ggtggaacct ggcagccatt cgatcaggtt
     2881 tttaccctgc acgtttgctt cacggaaggc cggatcggta gcttcccagt cattctgttg
     2941 cgcgacggaa taggccacgt tagtgtcgaa atagaagctc ttatcgccct ctttccacac
     3001 ttcctgaccc aattttaatt cagcataagt ttcacattcg ttgccaagac ggtatttact
     3061 ttgagcaccg gtagtctgga aacactgttg ttcaccgccg ctacctgtcc agccaatacc
     3121 ggaacgtgca tagccgtgga aatcaacagc cattgcctga gcagacatta cgcccgctgc
     3181 gacggcaacc gccagaggaa gtttgcgcag agtaatcatc attctatctc ctgagtcatt
     3241 gcttttcttt tttcacatca cctgtgacag gctttgtgtg ttttgtgggg tgcttaaacg
     3301 cccggctcct tatgcagtcg acgacatgca gtgccatcct cacggaacag atggcaacgc
     3361 tctggcggca ggccgatagc gaatgtggca ccttcttcta ccaacaccac gtcgttctgg
     3421 cggtacacca ggttttgacg aatggaaggg atctggatat ggatttgggt ttcgttgccg
     3481 agttgctcga cgacctgaac ttcaccctca aggatgacgt cagcgatatc actcggcagt
     3541 agatgttccg ggcgaatacc cagcgacata ttggctccaa cctggacatc acggctttca
     3601 actggcagcc agacttgctg acgatttggc atcggcagct ccacctgcac ttgatcgatt
     3661 gcggtggcgg tcacttttac cggcaggaag ttcatctttg gcgaaccgat aaatccggcg
     3721 acaaaacggt ctgccggata gtggtacagc tccagcggtt tcccaacctg cgccacgcga
     3781 ccggcgtcca gcaccacgat tttgtcggcc agcgtcatcg cttcgacctg atcgtgggtg
     3841 acgtaaatca ttgtgcggcc caggcgttta tgcagacggg agatttcgat acgcatttgc
     3901 acacgcagtg cagcatcgag gttggagagc ggttcatcga gcaaaaatac gcttggctcg
     3961 gccaccagcg tacggccaat cgccacacgc tgacgctgac caccggagag cgctttcggt
     4021 ttgcgatcca gcaaatgcgc cagttgcagc acttccgcca cctggttgac gcgttggtta
     4081 atcacctctt tttttgcgcc agccagtttc aggccaaatg acatgttttc tgctactgac
     4141 aggtggggat agagcgcgta agactgaaac accataccaa cgccgcgttc tgctggcgga
     4201 gtgtcattca tccgtttctc accgatgaac aggtcgccgc tggtgatcgt ctcaagcccg
     4261 gcaatcatgc gcagtaaagt cgatttaccg cagccagacg gtccgacaaa caccacgaat
     4321 tcaccttcat ggatatcgag attgatatct ttcgatacca cgacctcgcc ccaggctttc
     4381 gttacatttt gcagctgtac gctcgccatg cccttctccc tttgtaacaa cctgtcatcg
     4441 acagcaacat tcatgatggg ctgactatgc gtcatcagga gatggcttaa atcctccacc
     4501 ccctggcttt tttatggggg aggaggcggg aggatgagaa cacggcttct gtgaactaaa
     4561 ccgaggtcat gtaaggaatt tcgtgatgtt gcttgcaaaa atcgtggcga ttttatgtgc
     4621 gcatctccac attaccgcca attctgtaac agagatcaca caaagcaacg gtggggcgta
     4681 ggggcaagga ggatggaaag aggttgccgt ataaagaaac tagagtccgt ttaggtgttt
     4741 tcacgagcac ttcaccaaca aggaccatag attatgaaaa taaaaacagg tgcacgcatc
     4801 ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa
     4861 gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc
     4921 ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg
     4981 gaagagaaat tccctcaggt tgcggcaact ggcgatggcc ctgacattat cttctgggcg
     5041 cacgaccgtt ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa
     5101 gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg
     5161 attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg
     5221 aacccaccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt
     5281 aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct
     5341 gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg atattaaaga cgtggccgtg
     5401 gataacgctg gcgcgaaagc gggtctgacc ttcctggttg accttattaa aaacaaacac
     5461 atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg
     5521 atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt
     5581 gtaacggtac tgccgacctt caagggtcaa ccatctaaac cgttcgttgg cgtgctgagc
     5641 gccggtatta acgccgccag tccgaacaaa gagctggcga aagagttcct cgaaaactat
     5701 ctgctgactg acgaaggtct ggaagcggtc aataaagaca aaccgctggg tgcagtggcg
     5761 ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac
     5821 gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg
     5881 cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac
     5941 gcgcagactc gtatcaccaa gtaatgctgt gaaatgccgg atgcggcgtg aacgccttgt
     6001 ccggcctaca aaactgaaac gcatgtaggc ctgataagac gcatcagtgt cgcatcaggc
     6061 agttgttgtc ggataaggcg tgaaagcctt atccgtcctg gaatgaggaa gaaccccatg
     6121 gatgtcatta aaaagaaaca ttggtggcaa agcgacgcgc tgaaatggtc agtgctaggt
     6181 ctgctcggcc tgctggtggg ttaccttgtt gttttaatgt acgcacaagg ggaatacctg
     6241 ttcgccatta ccacgctgat attgagttca gcggggctgt atattttcgc caatcgtaaa
     6301 gcctacgcct ggcgctatgt ttacccggga atggctggaa tgggattatt cgtcctcttc
     6361 cctctggtct gcaccatcgc cattgccttc accaactaca gcagcactaa ccagctgact
     6421 tttgaatgtg cgcaggaagt gttgttagat cgctcctggc aagcaggcaa aacctataac
     6481 tttggtcttt acccggcggg cgatgagtgg caactggcgc tcagtgacgg cgaaatcggc
     6541 aaaaattacc tctccgacgc gtttaaattt ggcggcgaac aaaaactgca actgaaagaa
     6601 acgaccgccc agcccgaagg cgaacgcgcg aatctacgcg tgattaccca gaatcgtcag
     6661 gcgctgagtg acattaccgc cattctgccg gatggcaaca aagtgatgat gagctccctg
     6721 cgccagtttt ctggcacgca gccgctctac acactcgacg gtgacggcac gttgacgaat
     6781 aatcagagcg gcgtgaaata tcgtccgaat aaccaaattg gcttttacca gtccattacc
     6841 gccgacggca actggggtga tgaaaagcta agccccggtt acaccgtgac tactggctgg
     6901 aaaaacttta cccgcgtctt caccgacgaa ggcattcaga aaccgttcct cgccattttc
     6961 gtctggaccg tggtgttctc gctgatcact gtctttttaa cggtggcggt cggcatggtt
     7021 ctggcatgtc tggtgcagtg ggaagcattg cgcggcaaag cggtctatcg cgttctgctg
     7081 attctgccct acgcggtgcc atcattcatt tcaatcttga ttttcaaagg gttgtttaac
     7141 cagagcttcg gtgaaatcaa catgatgttg agcgcgctgt ttggcgtgaa gcccgcctgg
     7201 ttcagcgatc cgaccaccgc ccgcacgatg ctaattatcg tcaatacctg gctgggttat
     7261 ccgtacatga tgatcctctg catgggcttg ctgaaagcga ttccggacga tttgtatgaa
     7321 gcctcagcaa tggatggcgc agatccgttc cagaacttct ttaagattac gctgccgctg
     7381 ctgattaaac cgctgacgcc gctgatgatc gccagcttcg cctttaactt taacaacttc
     7441 gtgctgattc aactgttaac caacggcggc ccggatcgtc ttggcacgac cacgccagcc
     7501 ggttataccg acctgcttgt taactacacc taccgtatcg cttttgaagg cggcgggggt
     7561 caggacttcg gtctggctgc agcaattgcc acgctgctct tcctgctggt gggtgcgctg
     7621 gcgatagtga acctgaaagc cacgcgaatg aagtttgatt aagggagata acaaaaatgg
     7681 caatggtcca accgaaatcg caaaaagcac gtttgtttat tactcacctg ctactgctac
     7741 tttttatcgc ggcgattatg ttcccgctgc tgatggtcgt cgctatctca ctgcgccagg
     7801 ggaactttgc gatcggcagc ctgatcccgg agcaaatctc ctgggatcac tggaaactgg
     7861 ctttaggttt tagcgtggaa caggctgatg gtcgcattac gccaccgcca ttcccggtac
     7921 tgctgtggct atggaactcg gtaaaggtcg ccgggatttc cgcgattggc attgtcgcgc
     7981 tctctaccac ctgcgcctac gctttcgccc gtatgcgctt tccaggcaaa gcgacgctgc
     8041 tgaaaggaat gctgattttc cagatgttcc cggcagtact ttcactggtc gcgttgtatg
     8101 cgttgtttga tcgtctgggt gagtacattc cattcattgg cctgaatact cacggcggcg
     8161 taattttcgc gtatctgggt gggattgcgc tgcatgtctg gaccatcaaa ggctatttcg
     8221 aaaccatcga tagttcgctg gaagaagcgg ctgcgctgga tggtgcgaca ccgtggcagg
     8281 ccttccgcct tgtcctgttg ccgctgtcag taccgattct ggcggtggta ttcatcctgt
     8341 cgtttatcgc tgccattact gaagttccgg tcgcgtcgct gttactgcgt gacgtaaaca
     8401 gctacaccct ggccgtgggg atgcagcaat acctcaaccc gcaaaactac ctgtggggtg
     8461 actttgccgc cgctgccgtg atgtctgcat taccgatcac catcgtcttc ttgctggctc
     8521 aacgctggct ggtcaacggc ctgacggcag gtggtgtgaa aggttaaaga tgttgttctg
     8581 ccaatgttat gccgctgcac cctcaactta cgttatccca acttgtgact gttattcggc
     8641 gctccacgga gcgccttttt ttctttcgtc tgcaatctga atcgttcgcc ggttaatatt
     8701 tccatcatag agcttattat ttttacgtta tttgttttcc cacttacgat aattctcttt
     8761 cgtgctctga gtcacggcaa tagtattgtt ttcatcaatt tttgataatt atcacaatta
     8821 agatcacaga aaagacatta cgtaaacgca ttgtaaaaaa tgataattgc cttaactgcc
     8881 tgacaattcc aacatcaatg cactgataaa agatcagaat ggtctaaggc aggtctgaat
     8941 gaatacccag tataattcca gttatatatt ttcgattacc ttagtcgcta cattaggtgg
     9001 tttattattt ggctacgaca ccgccgttat ttccggtact gttgagtcac tcaataccgt
     9061 ctttgttgct ccacaaaact taagtgaatc cgctgccaac tccctgttag gattttgcgt
     9121 agccagcgct ctgattggtt gcatcatcgg cggtgccctc ggtggttatt gcagtaaccg
     9181 cttcggccgt cgtgattcac ttaagattgc tgctgtcctg ttttttattt ctggtgtagg
     9241 ttctgcctgg ccagaacttg gttttacctc tataaacccg gacaacacag tacctattta
     9301 tctggcaggt tatgtcccgg aatttgttat ttatcgtatt attggcggta ttggcgttgg
     9361 tttagcctca atgctttcac caatgtatat tgcggaactg gctccagctc atattcgcgg
     9421 aaaactggtt tcatttaacc agtttgcgat tattttcggg caacttttag tttactgcgt
     9481 aaactatttt attgcccgtt ccggtgatgc cagctggctg aatactgacg gctggcgtta
     9541 tatgtttgcc tcggaatgta tccctgcact gctgttctta atgctgctgt ataccgtgcc
     9601 agaaagtcct cgctggctga tgtcacgcgg caagcaagaa caggcggaaa gtatcctgcg
     9661 caaaattatg ggcaacacgc ttgcaactca ggcagtacag gaaattaaac actccctgga
     9721 tcatggccgc aaaaccggtg gtcgtctgct gatgtttggc gtgggcgtga ttgtcatcgg
     9781 cgttatgctc tccatcttcc agcaatttgt cggcatcaat gtggtgctgt actacgcgcc
     9841 ggaagtgttc aaaacgctgg gggccagcac ggatatcgcg ctgttgcaga ccattattgt
     9901 cggagttatc aacctcacct tcaccgttct ggcaattatg acggtggata aatttggtcg
     9961 taagccactg caaattatcg gcgcactcgg aatggcaatc ggtatgttta gcctcggtac
    10021 cgcgttttac actcaggcac cgggtattgt ggcgctactg tcgatgctgt tctatgttgc
    10081 tgcctttgcc atgtcctggg gtccggtatg ctgggtaccg ctgtcggaaa tcttcccgaa
    10141 tgctattcgt ggtaaagcgc tggcaatcgc ggtggcggcc cagtggctgg cgaactactt
    10201 cgtctcctgg accttcccga tgatggacaa aaactcctgg ctggtggccc atttccacaa
    10261 cggtttctcc tactggattt acgggtgtat gggcgttctg gcagcactat ttatgtggaa
    10321 atttgtcccg gaaaccaaag gtaaaaccct tgaggagctg gaagccctct gggaaccgga
    10381 aacgaagaaa acacaacaaa ctgctacgct gtaatcttcc tgtccagcac gccgcgccag
    10441 ttcggcgtgc tttcttttta ctcccgcttc agccgtttcg aattacacag ccacagagtg
    10501 atcaccagta acaggatcgc agccgagtag atcaacacat ccagtggcga tttatgatcg
    10561 acgatgatca agcgcacaat ggcggtgatc ccaatataga caaagtaacg tagcgggaag
    10621 tgaaaaccgg actgaaagta cttcacaatc agcgcgataa attcgaaata gagaaagtaa
    10681 accaccagtc cttctaccag ctcatatttg ctggtttgtt ctggcgcgaa cagcacatct
    10741 gccagatgca ccgtttcttt gccgaggaag acaaccaaaa tcaggccgag gcacagcagg
    10801 ccaagattga gtacggtctg caaaatggtg gagataaact ccacgcgcgg acgagagagt
    10861 gacgtcatag cagtacctcc ttcacagtgg cgagcttcct gtataacgca aaaatgtgac
    10921 ggagatctat attttttgtg tatgttttgt tcaacctgga tttggagcgc gcctccacca
    10981 aagccggata tacctacccg taggccggat aagccgcgcc tggcggcgca tccggcaaga
    11041 caaaccgcac ccaagccggt aattcaaaaa tcacccattc aggtgattcc cagccaccag
    11101 caatgcgcga aaaatcgaca atgttcctct ggaacaaatg aaacgtaacc tgataaaggt
    11161 ggtaaaaatg aagccttatt ttgctgcttt gatgttatca gtctctgttc ttcctgctta
    11221 tgctggtccg ctcggtactg ctgataaagc cgacctaccg caaagcaatg tctccagccc
    11281 aatgatggcc cagtcgctca ggcaatcaga cctgcaaccg atatcgacgg acaggaagac
    11341 agaatgtttt cgcctttaca caccagatcg caaaccgggg gtgaactgcg ttcctgatgg
    11401 ctcaacaggc cattaagaat gagggtgcca gtgattgcga aaactggcac ctttcccatg
    11461 acttagcgga aattgacgct acggtcgctg gtcatatcat acaactggaa cttacgccca
    11521 agttgctgac caccgtcacg cgtcagcggc gtccagccaa tcgccgcacg gctgcgtgtc
    11581 gggccagacg agaagagatc caacggtacc gagacataca cgcctttggt gaagtcccct
    11641 tcgccgtact cctcttttga aacattcgtg atcgtggcat agccacccac caccacgccg
    11701 ctatcaaagc gtttggcgat ctccagcgtg ccgcctttat cccctgccag atactgcccg
    11761 acgctggctt tcactaacac gtcctgggcg aaagatggcg tccagtaggc ggtcagatgt
    11821 ccggttttca cgctgtagtc ggtgaatttc atcatatctt ttgcactacg ccagtcgcgc
    11881 tgtttaacgt agttggcatc cagaccaaac gcccagttgc tgtccagcgg acgatacaac
    11941 acttcagccc ccgcaccgcc aaacatggtt tcgagataac ctccatagac ctgaccgtag
    12001 aagccgttgc ccagatgctg gaagtagttg gcttgcaggt tattcacata ggcatcgttc
    12061 tgcacatact cgcgcacatg ggtacgaacg cgcggtaagt gcgagtcctg tggcggattg
    12121 gtgtagttaa acttgtcgta gttgttggca agatttgcaa acaggctgcc gatggtcagc
    12181 agatggtccg tcagccacaa atctgccgtt cccatcacgc ccagctgata catgtaaaag
    12241 ttttccgggc caccgaccga ctggttcagc accggatcga tatggaaatc aaagcgtgat
    12301 ttgtcgatat accagccctg ctcggtggac tgcggaacca ctggctcgac gcgtttttgc
    12361 gccagcgtcg tttcgtggcc caacggctct ccggcgagat gatttttcag gctggcgaca
    12421 tcggtttccg tcgtcacctg cggcatgtta aggcgatttt ccgtaatgcg gatcgttttg
    12481 atcccatccg gcagatcgtt catcacgatc cgattagcac ggatgatccc ttcgcgcgaa
    12541 tcacgatatt tcacctgctc gccggtaaca tacagcgtat cgcctttcgc ctggatctgc
    12601 ggatcggcaa gtccggcatt gtatttcagc aacgttaact gattcgccac caccgagtgc
    12661 tgcaaaatgg catcctgcgg ctgcggttga tattgcgggc gggcgttatc gatgtaggac
    12721 gggcgcagat cgttaaagtt ggtgcgcagc gtgacgccaa acataaaggt gttgccacgt
    12781 tcatagctaa ggttaacgtc ggcccaatcg gtaacgcgat aaatcgcccc gacgttaaac
    12841 ttactttttt gctccagctt cccggcaaaa tcctgctgat aattattacc ttcatactcc
    12901 agtttcaggc gcagcggttg ccagggcgtc tggtattcca cgccgccaaa cagtgaggcc
    12961 gggccgtgga acatctgact gccgtcgata gatcccgctt gtttgtagct gttatcgcga
    13021 tagcaatatt tatcactggc tgaacagagc ggatttttca cattaccgct ggtgcccaga
    13081 tatccccagc ccaggccgag cgtaaaatcg aacggccccc aggctttgct ggcaacaaga
    13141 tattccgcat caaacagccc cgtaccgccg atatcccgcg caccaaccgc cacttgcggc
    13201 agccagtaac tctcttccca caaacgcagt ttgagatcga aggctttatc tttatacgtt
    13261 tgatcgccag agaacgcttc gacgctgctg tactgccggg tgcgcacgtc ggtgtaacgc
    13321 agcgttgttt ccagccacgg gaagagttgc actgaagctg agtaataacg gtactgatcg
    13381 ttatcgcgat agttcagact caactcccct tcccgtgcca tacgcgcggt gggagtttgt
    13441 aataatccta cgccaccgaa atccgactgc gacggaccaa tgggcgcagg atatgtttcg
    13501 ccgtagcaag ctgtgctaat gcccagcgcc agtaagctaa gcagatgtct ttttttcatt
    13561 attggggtat ccgctgcgtc agagtctgaa gaatgtcggc gttaagggca tcaggcgtct
    13621 cactccagac ggagtctgcg aggccaacat aaataatgct gccgggcatc ggctctacgt
    13681 gacgcttgtt ccagtaagcc accggcgctt tttgcgtgcg tccgtccggg taaacgaccc
    13741 acgcgtagct gcgatccgca ccgctgagca ggctctggtc agagagatag ctcgccacgt
    13801 cgcgacctgg agtgaatggc tgattgccag gatggctgat aagcccgaac aacgtgaccg
    13861 tggacggcgg tggtccgacc cacagcgtgt agttgccctg caacggcggg ttaccgcgtt
    13921 cggcaacgcg gacgatatcg ggatcaagat tgattttttg cctgcctgtc acttccaatg
    13981 cctggatttg ctggcgtaag gcgttaatgg cggcggcatc gtcggtgctg gaatctgcgc
    14041 cctgttctgc cagccgtgtc agcaacgcct gctgctgacg caatgctgcc gccgttgcca
    14101 gctcttcgct aatcaccgca ccaggccacc agctgtttgc cagtcgaggt tgccctacca
    14161 gatcgattaa atgctctgcg cccgttagcg ttttggcctc actgctgcca ttgctgaaca
    14221 ccttgacggt tcctgccgcg aagaccgatg acgctcccac actcaaaagc aacgcgacaa
    14281 tagtttgttt aatcattgtt ttgccgcctt gatgagagtg gttttcaccg ggaaataatc
    14341 cgcgccgaga tactgttccg actggcgaat ttgcccttcg ctgtcgatcc agtagcggtt
    14401 atgccagcgc gcctggtcgg tggagacttc ttcgtccagc acgcgaacca gggtttcatc
    14461 gctgccgacc ttgacggtat cggtgccatc ccatttgaag actgagcgtg cggtggcgta
    14521 gcgtacctgc tggtactcgg tccagcccat cgtgcgcgtc catgttgcac catcaacaat
    14581 ttgtgcgggt ttaatcagcg ggtcggcggc aaggttattc acttcaatca ggttgtcgcc
    14641 gccaagcaaa gtcttcacca gacgaccatg ttgtgtgaca agagtggctt gatcctgagt
    14701 gacccatttt tgttgtccgt cttcagcgaa ggccagcacc acaaacaatt gcggcccgcc
    14761 attaagctgc atgtactggc tggcgtaggg catattttga atatcatcgt ccgtcagctg
    14821 tacgcctggc gtgccgaaca gactgtccca cagtgaattg cccagctctt tagtcgtggc
    14881 tgaacaggcc tgtaataaca ggcagataag aatgagtgca ggtcgcttca cgactcttct
    14941 ccagaggggc agaataacca caccggagtg tggttatggt taatcccttc atatttcaag
    15001 ccacagatgc aatgactcga attattttgg ggatacatac tttattactg ggtactggtg
    15061 gttgtggttg ttgtggtccc ggtattagaa ccatcaccgc caccggttgc cgcgagcgcg
    15121 acacccaccg ccgagcttac ggtgctgacg ctggtcgcgg aggaactccc cgccgaaacc
    15181 gacgttgctg ccgcccctgc cgcttcgccc acctgtacag gtgcagccca cacagaagtc
    15241 gccgcaagcg cagatatggc aaaaatgcca tacagaactt ttttcataac aatttccctt
    15301 cattgaatga atggagattt acccaaaaac atttcgggcg ggatcgagta tacacaacta
    15361 aagcatgcga attacggcgc ggggaattag cgatggtgtt ttaggataat tggaatgaat
    15421 atcaaatata agtttcaact tatatttaaa taacaataaa aatcataaac ttaaaataaa
    15481 aacaaaagta attaatctgc gtattctccg aaataaacaa cgattaaaaa tctattttag
    15541 gattaacctc acggtatgat ttccgttaaa tcacagacaa ggggatttat ctgataaaac
    15601 aatgccgact tattgtcggc attgttttta aggttattta actcctcata ctatgcatta
    15661 atgtcgatat tggcatcagg ctccggttgc cggatgcggc gtaaacgcct tatccggcct
    15721 acatatcggc atcaggcacg catgatgatt aaccgcgcca cgctttatag cggttaatca
    15781 gaccattggt cgagctatcg tggctgctga tttctttgtc atctttcagc tctggcagaa
    15841 tacggttcgc cagttgttta cccaactcca cgccccactg gtcgaaggta aagatgttca
    15901 ggatcacgcc ctgagtaaag attttgtgct catacagcgc aatcaacgca cccaggctga
    15961 acggagtgat ttcgcgcagc aggatggagt tggtcgggcg gttaccttcg aacactttga
    16021 acggcaccac gtagtcaagc gttgccggat ctttaccctg atcgcgatat tcctgctcaa
    16081 ccacttcgcg ggatttacca aacgccagcg cttcggtctg ggcgaagaag ttagacagca
    16141 gtttctggtg gtgatcagag agcgggttat gggtgatagc cggggcgatg aaatcgcacg
    16201 gtaccatttt ggttccctgg tggatcagct ggtagaacgc gtgctgaccg ttagtgcctg
    16261 gttcacccca gataatcggg ccagtctggt aatccacaac gttaccgtta cggtcaacat
    16321 acttaccgtt ggactccata ttgccctgct ggaagtacgc cgcgaaacgg tgcatatact
    16381 ggtcatacgg cagaatcgct tcagtttccg caccaaagaa attgttgtac cagatgccaa
    16441 tcagcgccag caggacaggc aggtttttct cggcaggcgt ggtggagaaa tgcttgtcca
    16501 tcgcgtgtgc gccagaaagc agttcaacga agttatcaaa gccgatggag agaacaatcg
    16561 acaggccaat cgctgaccac aaagagtaac ggccgccgac ccagtcccag aactcgaaca
    16621 tgttggcagt atcaatacca aactcgccaa cggctttggc attggtggaa agcgccgcaa
    16681 agtgttttgc aacgtgtttt tcatcacctg ccgctttcag gaaccagtca cgcgcgctat
    16741 gggcgttggt catagtttcc tgagtggtga aggttttaga tgctaccagg aacagcgtgg
    16801 tttccgggtt tacttttttc agcacttcag cgatgtgagt cccatcgacg ttagaaacaa
    16861 agtgcatgtt caggtgattt ttgtacggac gcagtgcttc ggtcaccatg tacgggccga
    16921 ggtcagaacc accgatcccg atgttcacta cgtcagtgat tgctttgccg gtataaccct
    16981 tccactcacc ggagataatc gcttccgaga aggttttcat cttctccagc accgcgttga
    17041 cttccggcat tacgtctttg ccatcaacca gaatcggggt attgctacgg ttacgcagcg
    17101 ctacgtgcag cacggcgcgg ttttcagtgc ggttgatctt ctcgccagag aacatcgact
    17161 taatcgcgcc cgccagatcg cactctttcg ccagatcctg taatttcgcc agcgtctctt
    17221 cagtgatgcg gtttttggag taatccacca gcatctgatc gtcgaatgtt gcggagaact
    17281 tagaaaaacg atcgccgtct ttagcaaaaa gatcggcgat cgtaacgtct ttcatttcat
    17341 cgaagtgttt ctgtagtgcc tgccaggcag cggtctgcgt tggattgatg tttttcatta
    17401 gcaatactct tctgattttg agaattgtga ctttggaaga ttgtagcgcc agtcacagaa
    17461 aaatgtgatg gttttagtgc cgttagcgta atgttgagtg taaaccctta gcgcagtgaa
    17521 gcatttatta gctgaactac tgaccgccag gagtggatga aaaatccgca tgaccccgtc
    17581 gttgacaacc gccccgctca ccctttattt ataaatgtac tacctgcgct agcgcaggcc
    17641 agaagaggcg cgttgcccaa gtaacggtgt tggaggagcc agtcctgtga taacacctga
    17701 gggggtgcat cgccgaggtg attgaacggc tggccacgtt catcatcggc tacaggggct
    17761 gaatcccctg ggttgtcacc agaagcgttc gcagtcgggc gtttcgcaag tggtggagca
    17821 cttctgggtg aaaatagtag cgaagtatcg ctctgcgccc acccgtcttc cgctcttccc
    17881 ttgtgccaag gctgaaaatg gatcccctga cacgaggtag ttatgtctga aattgttgtc
    17941 tccaaatttg gcggtaccag cgtagctgat tttgacgcca tgaaccgcag cgctgatatt
    18001 gtgctttctg atgccaacgt gcgtttagta gtcctctcgg cttctgctgg tatcactaat
    18061 ctgctggtcg ctttagcgga agggctggaa cctggcgagc gattcgaaaa actcgacgct
    18121 attcgcaaca tccagtttgc cattctggaa cgtctgcgtt acccgaacgt tatccgtgaa
    18181 gagattgaac gtctgctgga gaacattact gttctggcag aagcggcggc gctggcaacg
    18241 tctccggcgc tgacagatga gctggtcagc cacggcgagc tgatgtcgac cctgctgttt
    18301 gtcgaaatcc tgcgcgaacg cgatgttcag gcacagtggt ttgatgtacg taaagtgatg
    18361 cgtaccaacg accgatttgg tcgtgcagag ccagatgtag ccgcgctggc ggaactggcc
    18421 gcgctgcagc tgctcccacg cctcaatgaa ggcttagtga tcacccaggg atttatcggt
    18481 agcgaaaata aaggtcgtac aacgacgctt ggccgtggag gcagcgatta tacggcagcc
    18541 ttgctggcgg aggctttaca cgcatctcgt gttgatatct ggaccgacgt cccgggcatc
    18601 tacaccaccg atccacgcgt agtttccgca gcaaaacgca ttgatgaaat cgcgtttgcc
    18661 gaagcggcag agatggcaac ttttggtgca aaagtactgc atccggcaac gttgctaccc
    18721 gcagtacgca gcgatatccc ggtctttgtc ggctccagca aagacccacg cgcaggtggt
    18781 acgctggtgt gcaataaaac tgaaaatccg ccgctgttcc gcgcgctggc gcttcgtcgc
    18841 aatcagactc tgctcacttt gcacagcctg aatatgctgc attctcgcgg tttcctcgcg
    18901 gaagttttcg gcatcctcgc gcggcataat atttcggtag acttaatcac cacgtcagaa
    18961 gtgagcgtgg cattaaccct tgataccacc ggttcaacct ccactggcga tacgttgctg
    19021 acgcaatctc tgctgatgga gctttccgca ctgtgccggg tggaagtaga agaaggtctg
    19081 gcgctggtcg cgttgattgg caatgacctg tcaaaagcct gcggcgttgg caaagaggta
    19141 ttcggcgtac tggaaccgtt caacattcgc atgatttgtt acggcgcatc cagccataac
    19201 ctgtgcttcc tggtgcccgg cgaagatgcc gagcaggtgg tgcaaaaact gcatagtaat
    19261 ttgtttgagt aaatactgta tggcctggaa gctatatttc gggccgtatt gattttcttg
    19321 tcactatgct tatcaataaa cgagcctgta ctctgttaac caacgtcttt atcgagaata
    19381 attgccttta atttttttat ctgcatctct aattaattat cgaaagagat aaatagttaa
    19441 gagaaggcaa aatgaatatt atcagttctg ctcgcaaacg aattcccgta cactgaactc
    19501 tctggctcac tgagggcacc atcatggcac tcccccgtat tacccaaaaa gagatgactg
    19561 aacgcgagca gcgtgaactt aaaacactgc tggatcgggc gcggattgcg catggtcgcg
    19621 tactgaccaa ctccgaaacc aacagcatta agaaagagta catcgataaa ttaatggtcg
    19681 agcgtgaggc tgaagcgaaa aaagcccgcc aattgaagaa aaagcaggct tataaaccgg
    19741 atcctgaagc gtcatttagc tggtcagcaa atacgtcgac gcgcggcagg cgctaatgaa
    19801 caatcaacgc ctgagatttt cactcgtcag tgtgaacaca attttcccct caccctaacc
    19861 cttttcccga aggggcgagg ggactgtccg agcacttttg ggccgttttc atcagtctga
    19921 ggcgttaatc agcgcccctt ctttttacgc cccggcgagg taaaacgctt accgttggac
    19981 gccgggcgac cgcctttttc cgccgttttt tccatcttaa cgactggacg tttgatgccc
    20041 gctgttttcg gtttggcctt cgctttgggc ttcgcctcgg aagaggaatt ttcaatgagc
    20101 ttaaagagat cgataagctc gtcgtcggtt aaatcgcgcc attctcccag cggaatgccg
    20161 cttaagctga cgttcataat gcgcgtacgt tccagctttt tcacttcata gccgaaatgc
    20221 tcgcacatgc gccggatctg acggttcaac ccctgcacca gggtaatgcg gaagacaaac
    20281 ggtgcttctt ttttgacttt gcactttttc gtcaccgtcc cgaggatcgg cactcccgcc
    20341 cccataccac gaataaattc atcggtaatc ggtttatcga ccgtcaccag atactctttc
    20401 tcatgatcgt tacccgcacg gaggatctta ttcaccagat cgccgtgatt ggtgaggaaa
    20461 atcagcccct gggagtcttt atccaggcgg ccaatcggga acacgcgttt gctgtggtta
    20521 acgaaatcga caatgttatc gcgctcgcca tcttcggtgg tgctgacaat accgacgggc
    20581 ttgttcaggg cgataagtac caaatcttcg gcttcccgag gctcaatcaa ctgaccgttt
    20641 actttcacaa tgtcgccggg tttcacctga tcaccaatgg tggctcgctt gccattaagg
    20701 aacacattgc cttgctcgat atagcgatcc gcttcgcggc gtgagcaaat tccgctttcg
    20761 ctgatgtatt tatttaaacg gactgatgag tcgggcagca taaattctcc tgtaaaacgc
    20821 ggaatatacc gtacctctta gcggggaaaa acagggttat gcggcaacgt cgaaattaaa
    20881 tgtgatctgc cacgcctttt atcacaaaag tgtctccgga agtacgatgc aaatttgcct
    20941 ggcaaaataa ggataatcaa catattacga atcgctgcac acctgtgctg atgactacta
    21001 cgcagagaaa caaatgtgca agagaaagcg ctatttctcc ccatgcaaag agtgaaaaat
    21061 cgctttgaaa ggccctcata atggggttat atcagatcaa gcggtgagaa agcgtatttg
    21121 cacaaatgtg cagaaccagg aggcggcaat ggaaaacagt gacgatatcc gtttgattgt
    21181 gaagattgcc caactctatt acgaacagga tatgacgcag gcgcaaatcg cgcgcgaact
    21241 gggtatttac cgcaccacca tcagccgctt gcttaaacga ggccgcgatc agggaattgt
    21301 caccatcgcc atcaactatg actacaacga aaatctctgg ctggagcagc aactgaagca
    21361 aaagtttggc ttgaaagacg ttgtggtggt gtcgggaaat gatgaggatg aagagactca
    21421 actggcgatg atggggttac acggcgcgca actgctggat cgcttgctgg agcctggcga
    21481 tattgtcggt ttttcctagg gtcgcgcggt gagcgcactg gttgaaaact tgccgcaagc
    21541 ggggcaatcg cggcagttaa tctgcgtgcc aattattggc ggaccgtccg gtaaactcga
    21601 aagccgctat cacgtaaaca cattaaccta cagcgtggca gcgaagctga aaggggaatc
    21661 gcatctcgcg gattttccgg ctctgctgga taacccctta attcgtaatg ggatcatgca
    21721 gtctcagcac tttaaaacca tctctgccta ctgggataat ctggatgtcg ccctggtggg
    21781 aattggctca ccggccattc gcgacggcgc taactggcat gcgttttatg gtggtgaaga
    21841 gagtgacgac ctgaaagcag gcgcggtatt ccattggcat tgccatgagt gaagaaaaat
    21901 acagcggaat tgttggtgca ctgcgtggaa aatatattaa ttgtctggta acgaatagca
    21961 gcacagctga actattactg aaataaaaat cttcccgatg tttaccgtaa taaaaattac
    22021 ggcgggtccc tattatctaa aatcaggagt ctgttatgca aacgtggtta aatttgcagg
    22081 ataaaatcat tatcgtcacc ggcggcgcat ccggtattgg tctggcgatt gtagaggaat
    22141 tattagcaca aggcgcaaac gtacagatgg tagatattca cggtggcgat ggtcaatatg
    22201 aagttcataa aggctatcag ttttggccga ccgatatttc cagcgccaaa gaggtaaatc
    22261 atacggtagc ggaaattatc cagcgttttg gtcgcatcga cggtctggtc aataacgccg
    22321 gggtcaattt cccgcgtctg ctggtcgatg agaaagcgcc tgccgggcag tatgaactca
    22381 acgaagctgc attcgaaaaa atggtcaata tcaaccaaaa aggcgttttt ctgatgtcgc
    22441 aggcggtggc gcgccagatg gtcaaacaac atgatggcgt gattgtgaat gtttcctcag
    22501 aaagtggact ggaaggctca gaaggccaaa gctgttacgc cgctaccaaa gccgcgctca
    22561 atagcttcac gcgctcctgg tcgaaagagc tgggtaaaca cggtatccgt gtggtcggta
    22621 tcgcgccggg gattctggaa aaaaccgggc tacgcacgcc ggaatatgaa gaggcgctgg
    22681 cgtggacgcg caatatcacc gtcgagcagc tgcgtgaagg ctataccaaa aacgccattc
    22741 ctattgggcg cgccggaaga ttagcagaag tcgctgattt tgtttgttat ctgctgtctg
    22801 aacgcgccag ctacatcacc ggagtaacca ctaacattgc gggcggcaaa acgcgcggat
    22861 aaggaggctt tatggtcaac gccatttttt gtgcccacgg caaattagct tgcgccatgc
    22921 tggaatcggt tcagatggtg tacggcgacg ccaatgtcga ggcggttgaa tttgtaccag
    22981 gcgagaacgc cggagacatc gtagcaaagc tggaaaagtt agtaagcatt cacaatcaag
    23041 atgagtggct gattgccgtg gatttacagt gcggcagccc gtggaatgcg gcagcgatgc
    23101 ttgcgatgcg caatcctcgt ctacgggtaa tcagcggcct ttccctgccg ctggcgctcg
    23161 aactggttga taaccaggac agcatgaatg ttgatgaact gtgtgaacac ctgacgcaaa
    23221 tcgcaaagca aacctgcgtt gtctggaaac aactggcaac ggcagaggaa gatttctgat
    23281 gaacattacg ctcgcccgca ttgatgaccg tctgatccac ggccaggtca ctaccgtctg
    23341 gtcgaaagtg gctaacgccc agcgcattat tatctgcaat gacgaagttt ataacgatga
    23401 agtgcgccgg acattattgc gtcaggctgc gccaccggga atgaaggtta acgtggtcaa
    23461 tattgaaaaa gccgtcgcgg tttatcacaa cccgcaatat taggacgaaa ccgtttttta
    23521 tcttttcact cgcccacagg atgccttagc gatggttcgc cagggggtaa aaataggcac
    23581 cctgaatatt ggcggcatgg cctggcggcc cggtaaaaaa cagttaacca aagcggtttc
    23641 attagatgat gatgatatta acgcatttca tgagttaaat aatcttggcg tcataatgga
    23701 tttacgcgtc gtagcgtcag atccctcaat caatattatc gacaaaataa acgaacagtt
    23761 aatcgcaaat taaataatag cgcctgtgta catatgcttt aacaggcagg gatgacgaca
    23821 cttaaaaggt gacaaatcat ggaaataagt accctacaaa ttattgctat atttcttttt
    23881 tcctgtattg ccggaatggg cagcgtgctg gatgaatttc agactcatcg cccgttaatt
    23941 gcctgtacgg tgattggttt aattctcggt gatttaaaaa ccggaataat actcggtggt
    24001 acgctggaat tgatagctct cggctggatg aacgtcggcg cggcgcaatc tccggattct
    24061 gcactcgcca gcataatctc cgccattctg gttatcgttg gtcagcaaag catcgccacc
    24121 ggaatcgcca tcgcgttgcc tgtggctgcg gcaggccaag tgctgacggt gtttgcccgt
    24181 accatcaccg tagtgttcca gcacgcggcg gataaagcag cggaagaggc gcggtttcgc
    24241 actctcgata ttctgcatgt ctccgcgctt ggcgtgcagg cgctgcgcgt tgctattccg
    24301 gcactgattg tctcactgtt cgtcagcgcc gatatggtca gcaatatgtt aaacgccatt
    24361 cccgaatttg tgacccgtgg actgcaaatt gctggcggtt ttatcgtggt ggtcggttac
    24421 gccatggtgc tacgtatgat gggcgtgaaa tatttgatgc ctttcttttt cctcggtttc
    24481 ctcgcaggtg gctacctcga tctcagcctg ctggcgttcg gtggcgtcgg cgtgatcatg
    24541 gccctggtct acatccagct aaatccacag tggcgtaaag ctgaacctca actccaggcc
    24601 accgcctcca ccgcccttga ccaacttgac gattaacgga gccatcatgg aacagagaaa
    24661 aattacacgc agcgatctgg tgagcatgtt tctgcgctcc aacctgcaac aggcgtcctt
    24721 taactttgaa cgtattcacg gactgggctt ttgctacgac atgatccccg ccatcaagcg
    24781 actttaccca ttaaaagagg atcaggttgc ggcgctcagg cgacacctgg tgttcttcaa
    24841 taccacgcca gccgtatgtg gcccggtcat cggcgtcact gccgccatgg aagaggcgcg
    24901 ggccaacggc gcggaaattg atgacggtac cattaacggc atcaaagtcg gtctgatggg
    24961 accattggca ggagttggcg atccactggt ctggggaacg ctgcgcccga ttaccgctgc
    25021 gctcggcgca tctctggcac tttctggcaa catcctcggc ccgctgctgt ttttctttat
    25081 tttcaatgcg gtgcgtctgg cgatgaagtg gtatggccta cagctcggct ttcgcaaagg
    25141 ggtgaatatc gtcagcgata tgggcgggaa tgtgctgcaa aaactcaccg aaggcgcgtc
    25201 gattctcgga ctgtttgtga tgggcgtgct ggtcaccaaa tggacgtcaa tcaacgtacc
    25261 gttggtggtt tcacaaacgc atgccgccga tggctccacc gtcatcatga ccgtgcagaa
    25321 cattctcgac caactttgcc ctggtttgct ggcgctcggt ctgacgctac tgatggttcg
    25381 tctgctcaac aaaaaaatta acccggtatg gctgattttc gccctgtttg gcttagggat
    25441 tatcggcaat gcgctgggct tcctgtcctg attcttcgcc ccggcacgac tgccggggcc
    25501 atcgctcaac atgaggtggt ttatgaaaac aacagctctg cgtctttatg gtaaacgtga
    25561 tttacgcctg gaaacctttg accttcctga aatgcaggag gatgaaatcc tcgcgacggt
    25621 ggtcactgac agcctgtgcc tctcttcctg gaaagaggca acctgggtga aaaccataaa
    25681 aaagtacccg acgatgtggc gaccaaccca atcatcatcg gccacgagtt ttgcggcgat
    25741 attctggccg tgggtaaaaa gtggcagcac aaattccagc cgggtcagcg ttatgtgatt
    25801 caggccaacc tgaaactccc tgaccgcccg gactgccccg gctactcctt cccgtgggtg
    25861 ggcggcgaag ccacgcatgt ggttattccc aacgaggtca tggaacaaga ttgcctgctg
    25921 gcatacgacg gcgaaaccta ttttgaaggc tcgctggttg aaccgctttc ctgcgtgatt
    25981 ggcgcgttca acgccaacta tcatcttcag gaaggtagtt ataaccacac gatggggatt
    26041 cgcccgcaag ggcgcacgct gatcctcggc ggcaccggac caatgggatt gttggcgatt
    26101 gattatgcgc tgcatggacc agttaacccg tcgctgctcg tcattaccga taccgacaac
    26161 gacaaattga gttatgcgcg caagcactat ccatcagaac cgcaaacact gattcattat
    26221 ctcaatgcca ccgatgcagc atttgatacg ttaatggcgc tgagtggcgg tcacggcttc
    26281 gatgatattt tcgtctttgt gcctaatgaa ggactggtga ctctcgcctc ttccttgctg
    26341 gcgacagatg gttgcctgaa tttcttcgcc ggaccgcagg ataaacattt cagcgcgcca
    26401 attaatttct acgatgtgca ttatgcattt acccactacg tgggcacgtc aggcggcaat
    26461 accgacgaca tgcgcgcggc ggtcaaactg attgaagaga aaaaagtgca ggccgcaaaa
    26521 gtggtaacac atattcttgg gctgaatgcc gcgggcgaaa ccacgcttga attgcctgcc
    26581 gtcggcggcg gcaaaaagct ggtgtatacc gggaaatacc tgccgctgac gtcactcacg
    26641 cagattcagg atcaagcact ggcggcgatt ctggcgcgtc atcaggggat ctggtccggt
    26701 gaggcggagc aatatctgct cactcatgca gaggcaattt cccatgatta atcgcgctac
    26761 ttaactctgc atgtcgctgg ctggtcgccg cggcaacttc gcgacctgat gtgcggctga
    26821 ttgctgaagc ggcccaatgt gcacgagaag gataaaaaaa catcagcgcc ttaaggggcg
    26881 ctcaaattgg tggcaaagta cacattgttt atgccggatg cggaataaag ccttattcgg
    26941 cctacataat ctcgaaaatt caatatattg cagttatctg gtaggcctgg taagcgtagc
    27001 gcatcaggca atgctgtgtt tgtcatcagt ctcagcgccc ggtgcaatac gtgacatcgt
    27061 gagccatgtc agtaagtggt taaattcgca gtggtagcat gccttccgtg atttcttact
    27121 aaccggagaa gtaatggaac tgcttttatt gagtaactcg acgctgccgg gtaaagcctg
    27181 gctggaacat gcactgccgc taattgctga acagttgcag ggtcgccgct cagcggtgtt
    27241 tatccctttc gctggcgtaa cgcagacctg tgatgattac acagcgaaaa cggctgcggt
    27301 tctcgctccg ctgggtgttt ctgtcaccgg tattcatagc gttgtcgatc ccgttgccgc
    27361 gattgaaaat gctgagatcg tgattgtcgg ggggcgggaa tactttccag ttgctgaaac
    27421 agtgccgcga gcgcgggctg ctggcaccaa ttactgacgt ggttaaacgt ggtgcgctgt
    27481 atattggctg gagcgcaggc gctaaccttg cttgcccaac cattcgtacc accaacgata
    27541 tgccgattgt cgatccgcaa ggtttcgatg cgctaaatct gttcccgctg caaatcaacc
    27601 cgcacttcac caacgcgctg ccggaaggcc ataaaggtga aacccgtgag cagcgtattc
    27661 gcgaactgct ggtcgtcgcg ccagaactga cgattattgg tctaccggaa ggtaactgga
    27721 tcacggtgag taaaggtcac gctacgctgg gtggcccgaa caccacttat gtgtttaagg
    27781 ccggtgaaga agcggtgccg ctggaagcgg gtcaccgttt ttaagtgaat tgccggatgc
    27841 atgatccggc atcttgttca tagtgcctga tgcgacgctt gacgcgtctt atcaggccta
    27901 caggattcac aatattaata ctcgtcccgt ccttcatctt catccggctg ttccagcaca
    27961 ctgtaagcca ccgaacaaaa cagcgagttc agacgctgca tatctcccag taagcctaaa
    28021 tgtagcgaac tggtttcaat gctctgcacg ttttgctgat gcaggcgatc gacgtgggcg
    28081 tgggaatagc ggcgattaag aatgcgaaag cgatgcttgc tgcggcgcaa acgacgagcg
    28141 ctggtgacat cgccagagaa gaacaccgac attgccagct ttaaattact gagcaattgc
    28201 tcatagagcg catccagttc cttcaaccca tcaagcgaaa atgcccgccg tgctgccagc
    28261 gatttatcag caatttcgct gcccatgcgc tcgacgatat cggaagcctg ttcaaggttg
    28321 agcgacattt caataatctc cgcccagcga cgcgactctt cttctgccag ttcctctttt
    28381 ggcattcgcg ccagatacag cttaatggcg gtgtagagaa cgttgatatc atctgccagc
    28441 ttacgcagct ctttctcctg ccgtggctcg ccgtgcatca ctttattcag cccttccatc
    28501 atctgttcca tggcgtcgcc aatgcgcagg gtttcgcgcg cggcgttcgc cagagcaagc
    28561 gtgggcgtat ccagcgcgct gacatccaga tgtttaggcc gtagctgggt atccagttcc
    28621 ggttcatcgc gaataatcgt tttgcaaaac cgtgccatcg ggtcaacaaa tggcagcatg
    28681 accaggcaac gtacaaggtt gtagaagacg tggaaataga tcaccagttc cgcttttggc
    28741 aatgacaact tccccattgt ctctgccagc aaatggacaa acggcaggat aatcaggcta
    28801 cccaccagct taaacagcaa actacccagc gcgacacggc gggctgcggc attggcggca
    28861 ctgttgttga gcatcgccag caggccggaa ccgaggttag caccaatcac cagacagagc
    28921 gccacgggga aggagataat gcctgcggcg gtcagagtcg cggtcagcag tacagcagca
    28981 aggctggagt agctgataat ggcgaacatc gcgccaatca gcgcatccag cagaatatcg
    29041 ccggtcagcg aggcaaagat cacctgaacg ccgtttgcct gggtgatcgg cgttacggcc
    29101 tgcacaatca actccagcgc cagcaaaatc agcccaagac caataccgac gcggcccagt
    29161 tgcccggcgc gtgactgttt gcgtccgagg aaaaaaatca cgccgataaa aataagtaac
    29221 ggtgacagcc aggataagtc gaaggtgaga atacgcgcca ttagcgccgt cccgacatct
    29281 gcacccagca caatcaccag agccggtgcg agggctacca gatcctgagc gacgaacgag
    29341 gtcaccagca tggtggtggc attactgctc tgtaccagtg cggtaacgcc gatccccgcg
    29401 caaaaggcga gcggcttttt ttcgacgctc cggctaagga cagtacgcaa acgcgcgccg
    29461 aagacgcgca ttacgccggt tcgaacaata tgagtccccc agaccagcag ggcgacggca
    29521 gaaagcaggt gaagcagcgt taacacgagt tcaggttctc cttatcgtta tatgttcctg
    29581 aagcaattga atgaccaaag gaccggaaac acgctgactg gcatctttac tgtgcctttt
    29641 gacattcgtt tttccggcta agattattcc agcctgtttc cagtataagt gtttcatatt
    29701 aataaagaga cagggcactc attgagcgcc ctgtttgttg taaggaaaag tgacagattt
    29761 gtgaatcagt ccgcgtcata ccccagattc ggtgccagcc agcgctcaac atcggagacg
    29821 ctcatacctt tacggcgggc atagtcttca acctgatcgc gctgaatttg cgccaccgcg
    29881 tagtacttgc tgtccgggtg gctgaaatac caacccgaaa ccgatgcacc aggccacatg
    29941 gcgaaagatt ctgtgagttt catgccagta tgtttttcca cttccagcag ctcccagatg
    30001 gtggcttttt ccgtatgttc cgggcaggcc gggtagcccg gtgccggacg gatgccctgg
    30061 tagttttcgc ggatcagttc ttcgttgctg aggttctcgt tcggcgcata gccccagtag
    30121 actttacgca cacgctcatg gagatactcc gcaaaggctt cggctaaacg gtcggcaagc
    30181 gctttcacca tgattttgtt gtaatcatcg tgctgcgctt caaaggcatc agccagtgcg
    30241 tcctcttcca gcccgccagt cacggcaaat gcgccgatgt aatctgcttt accagaaagc
    30301 ttcggcgcaa cgaagtcagc gagacagtag ttagcgaatc ccgttttttc ggtctgttga
    30361 cgcagatggt ggctgacgtt gatcacatgg gtgcgcgttt cgtcacggta gatttcaatg
    30421 tcatcgccca cacggtttgc cgggaacaag cccaccacgc cacgcggatt cagcgttttc
    30481 tcggcgctta atttatccag catgtcgttg gcgtctttaa acagccgctg cgcctcaacg
    30541 cccaccactt catcttccag aatgcgcgga tacttcccgg ccagcgacca ggtcataaag
    30601 aacggtgtcc agtcgatgta attacgcaga gtttcgatgc tggcttcaac ttcctgcacg
    30661 ccgagacggt gcgccaccgg cggcgtgtaa gcctgccagt caaaagcgaa gtcgttatcg
    30721 cgcgccgctt ccagcgtgac cggtggtgtg cgcggtttct tgcgcccgtg ctgaatacgt
    30781 acggtttcat actccttgcg ggtacgagcg acaaaatcat cgcgctgagt atcggaaagc
    30841 agcgccgcca ccacaccaac ggtgcgcgag gcattctgca catacaccgt cgggccgctg
    30901 tagttctgct caattttcac cgccgtgtgc gcttttgagg tcgtcgcgcc accgatcagt
    30961 aacggaatgg tgaagccctg acgctccatc tctttcgcca cgttaaccat ctcgtccagc
    31021 gacggcgtga taagccccga aagtccaatc agatcagcat tcacttcttt agcggtacgg
    31081 agaatttttt ccgcaggcac cataacgccg agatcgacaa tttcgtagtt gttacattgc
    31141 agcaccacac caacgatatt tttaccgatg tcgtggacgt cgcccttcac ggtggcgatc
    31201 accatcttgc cgttggtttt gccctgctct ttgctggctt caataaacgg ttcgaggtag
    31261 gccactgcct gtttcatgac gcgcgccgat ttgaccacct gtggcaggaa cattttcccc
    31321 tcgccaaaca gatcgccgac cacgttcatg ccgtccatca acgggccttc aatcacttca
    31381 atcgggcgcg tagcctgctg gcgggcttct tcggtatcct gctcgataaa ctcagtaatg
    31441 cctttgacca gcgagtattc cagacgttta ttcacttccc acgagcgcca ctccgcctgc
    31501 tgggcgttag cggtgtcgtc ggttttgctg ccgcgatatt tctcggcaag ctccagtaaa
    31561 cgttcagtgc catcatcgcg acgattaaga atcacatctt ccaccgcgtc gcgcagttca
    31621 gcgggcaggt cgtcgtaaat cgccagttgc ccggcgttga cgatccccat atccatgcca
    31681 ttgcgaatag cgtagtagag gaacaccgcg tgaatggctt cgcgcaccgg ttcgttgcca
    31741 cggaatgaga aagaaacgtt agatacgccg ccggaaatca gcgcgtgcgg cagttcgcgt
    31801 ttgatgtctt cgcaggcgcc gataaagtcc tgcgcatagt tgttgtgctc ttcaatgccg
    31861 gtggcgaccg cgaagatgtt cgggtcgaag atgatatctt ctggcgggaa gccaacctct
    31921 tcggtgagga ttttgtacgc ccgacggcaa atctcgattt tccgtgcgcg ggtatcggcc
    31981 tgtccctgtt cgtcaaaggc cattaccacc accgctgcac cgtaacgacg caacagtttc
    32041 gcatgatgga taaaggcgtc gacgccctct ttcattgaga tagagttaac aatgcctttg
    32101 ccctgaatac acttcagccc cttttcgatg acctcccatt ttgaggagtc gatcataatc
    32161 ggcacgcgag cgatatccgg ttcaccggca atcagattga gaaaacgcac catcgccgct
    32221 tcggcgtcga gcatcccttc atccatattg atatcgataa tctgcgcgcc gttttccacc
    32281 tgctggcggg ccacatccag tgcctcgctg tatttctctt ctttgatcag gcgcttgaac
    32341 ttagcggaac cggtgacgtt ggtgcgttca cccacgttca caaacaggct atcttcgcca
    32401 atgttcagcg gctccaggcc ggacaaacgg caggctacgg ggatttccgg cagtttgcgc
    32461 ggcgctaatc cttctactgc acgactcatc gctgcaatat gttgtggcgt ggtgccacag
    32521 cagccgccga cgatattgag aaagcccgct tccgcccatt cacgtatttg ttttgccatc
    32581 gtgtcggcgt cgagatcgta ctcaccaaag gcgttgggta gcccggcgtt cgggtgcgcg
    32641 gtgacgtagc attccgcaat ccgtgacagc tcctgcacgt actggcgcag ttcatcgggc
    32701 cccagcgcac agttcaggcc aaaggtcagc gcttcggcgt ggcgcaatga gttgtaaaat
    32761 gcttcggtgg tctgcccgga gagcgtgcgc ccggaggcgt cggtgatggt gccagagatc
    32821 ataatcggca gctcaacgcc cagcgtttca aactccgttt ttaccgcaaa taccgccgct
    32881 ttggcgttaa gggtgtcgaa aacggtttca atcaggatca gatccgcacc accttccacc
    32941 agcgctttgg tggactctcg ataagccacc accagcccgt caaaagtgat attacgaaat
    33001 gccggatcgt tgacgtccgg agaaatagac gccgtgcggt tggttgggcc gagaacaccg
    33061 gcaacgtagc gcggtttctc cggcgtgcgc gcggtccact cgtcagcaca ggcgcgcgcc
    33121 agtttcgccg ccgcaaagtt gatttccgcc gacagggatt ccatctggta atccgccatc
    33181 gcaatggtcg tggagttgaa ggtgttggtt tcgatgatat ccgcgcccgc ttcaaagtag
    33241 gcgttgtgga tagcggcgat cacttccggt ttactgagta ccagcaggtc gttgttgcct
    33301 ttgaggtcgc atggccagtc ggcaaagcgt tcaccacgaa aatcggcttc gttcagtcga
    33361 tagctctgga tcatggtgcc cataccgccg tccagcacca gaatacgttc atttaactgc
    33421 gcacgcagtt gttccacttt gctgctcaca cttgctcccg acacgctcaa cccagattta
    33481 ataaaaattc aacaaaccat actggcataa acgcatctgt ggtaaaagcg accaccacgc
    33541 aacatgagat ttgttcaaca ttaactcatc ggatcagttc agcaactatt gcattaacta
    33601 acaataaaaa tgaaaatgat ttccacgata cagaaaaagg agactgtcat ggtcgcaccc
    33661 attcccgcga aacgcggcag aaaacccgcc gttgccaccg caccagcgac tggacaggtt
    33721 cagtctttaa cgcgtggcct gaaattactg gagtggattg ccgaatccaa tggcagtgtg
    33781 gcactcacgg aactggcgca acaagccggg ttacccaatt ccacgaccca ccgcctgcta
    33841 accacgatgc aacagcaggg tttcgtgcgt caggttggcg aactgggaca ctgggcaatc
    33901 ggcgcacatg cctttatggt cggcagcagc tttctccaga gccgtaattt gttagcgatt
    33961 gttcacccta tcctgcgcaa tctaatggaa gagtctggcg aaacggtcaa tatggcggtg
    34021 cttgatcaaa gcgatcacga agcgattatt atcgatcagg tacagtgtac tcacctgatg
    34081 cgtatgtccg cacctatcgg cggtaaattg ccgatgcacg cttccggtgc aggtaaagcc
    34141 tttttagccc aactgagcga agaacaggtg acgaagctgc tgcaccgcaa agggttacat
    34201 gcctataccc acgcaacgct ggtgtctcct gtgcatttaa aagaagatct cgcccaaacg
    34261 cgcaaacggg gctattcatt tgacgatgag gaacatgcac tggggctacg ttgccttgca
    34321 gcgtgtattt tcgatgagca ccgtgaaccg tttgccgcaa tttctatttc cggaccgatt
    34381 tcacgtatta ccgatgaccg cgtgaccgag tttggcgcga tggtgattaa agcggcgaag
    34441 gaagtgacgc tggcgtacgg tggaatgcgc tgactttttc tggcgggcag aggcaatatt
    34501 ctgcccatca tacctgagtg gcaatagaat aagggtgtct gttaatcgca ttgacgccaa
    34561 aataacttaa tgtcatacac ttcacacact tttattaact cagcattatt tttaaacatc
    34621 aaaccactta attattaaca aaatcgcaag atctcattcc accgaaactt ttacggctgc
    34681 attatgagat tgagcgattt atgatatttc tttagttgta atcatattaa attttttcac
    34741 tgttctgata gttaaaattt aagacatcaa taaacaatga gatatttaaa tgattactcg
    34801 tattcctcgt agttctttct ctgcaaatat taataataca gcccagacaa atgaacacca
    34861 aaccctgagt gaattgtttt ataaagaact cgaggataaa ttttctggca aggagctggc
    34921 gacgcctcta ttaaaaagct tctcagagaa ctgtagacaa aatggtcgtc atatctttag
    34981 caacaaggat tttgtcatta aattttccac gtccgtctta caagctgata agaaagaaat
    35041 tacgataatt aataaaacga aaacacgaca ctcactcaaa ccattgctcc aatatttgaa
    35101 gaatacctaa tggaaatttt acctcaacgc tcagacactc ttgataaaca agaattaaac
    35161 ctaaaatcag atagaaaaga aaaagaattc ccaagaatta aacttaatgg tcaatgctat
    35221 tttccggggc gaccccaaaa ccgtatagta tgccgacaca ttgctgcaca atatattaat
    35281 gatatttatc agaatgttga ttacaaaccc catcaagatg attattcttc agctgaaaaa
    35341 tttctcactc acttcaacaa aaaatgcaaa aaccagactt tggcgttgat ttccagccgt
    35401 cctgaggggc gttgcgttgc tgcctgcggt gatttcgggc tagttatgaa agcatatttt
    35461 gacaagatgg aatcaaatgg catcagtgtt atggcagcca tattactggt ggataaccat
    35521 gctttgacgg tccggctaag aataaagaac acaactgaag gatgtaccca ttacgtggtt
    35581 tcggtttatg atcctaatgt aactaacgac aaaataagaa tcatgagcga aagcaaagag
    35641 gatattaaac actattctct gatggatttt atgaatgtag attatagcct cctgaaatgg
    35701 tcaaatgatc atgttattaa ccaatctgtt gcaataattc cagcacttcc gaaagaacag
    35761 ctattgatat taaaaggat
//
LOCUS       DAJVRF010000026        35431 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJVRF010000026 DAJVRF010000000
VERSION     DAJVRF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.026"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(159..371)
                     /gene="rpmE"
                     /locus_tag="OQN13_001182"
     CDS             complement(159..371)
                     /gene="rpmE"
                     /locus_tag="OQN13_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCS1614593.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            574..2772
                     /gene="priA"
                     /locus_tag="OQN13_001183"
     CDS             574..2772
                     /gene="priA"
                     /locus_tag="OQN13_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCS1614594.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2928..3953
                     /gene="cytR"
                     /locus_tag="OQN13_001184"
     CDS             2928..3953
                     /gene="cytR"
                     /locus_tag="OQN13_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCS1614595.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4045..5004
                     /gene="ftsN"
                     /locus_tag="OQN13_001185"
     CDS             4045..5004
                     /gene="ftsN"
                     /locus_tag="OQN13_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCS1614596.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5097..5627
                     /gene="hslV"
                     /locus_tag="OQN13_001186"
     CDS             5097..5627
                     /gene="hslV"
                     /locus_tag="OQN13_001186"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCS1614597.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5637..6968
                     /gene="hslU"
                     /locus_tag="OQN13_001187"
     CDS             5637..6968
                     /gene="hslU"
                     /locus_tag="OQN13_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCS1614598.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7035..7961
                     /gene="menA"
                     /locus_tag="OQN13_001188"
     CDS             7035..7961
                     /gene="menA"
                     /locus_tag="OQN13_001188"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCS1614599.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8054..8539
                     /gene="rraA"
                     /locus_tag="OQN13_001189"
     CDS             8054..8539
                     /gene="rraA"
                     /locus_tag="OQN13_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCS1614600.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8624..8869)
                     /gene="zapB"
                     /locus_tag="OQN13_001190"
     CDS             complement(8624..8869)
                     /gene="zapB"
                     /locus_tag="OQN13_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCS1614601.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9295..10140
                     /gene="glpF"
                     /locus_tag="OQN13_001191"
     CDS             9295..10140
                     /gene="glpF"
                     /locus_tag="OQN13_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCS1614602.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10163..11674
                     /gene="glpK"
                     /locus_tag="OQN13_001192"
     CDS             10163..11674
                     /gene="glpK"
                     /locus_tag="OQN13_001192"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCS1614603.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            11804..12814
                     /gene="glpX"
                     /locus_tag="OQN13_001193"
     CDS             11804..12814
                     /gene="glpX"
                     /locus_tag="OQN13_001193"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCS1614604.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12911..13657
                     /gene="fpr"
                     /locus_tag="OQN13_001194"
     CDS             12911..13657
                     /gene="fpr"
                     /locus_tag="OQN13_001194"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCS1614605.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13662..14090)
                     /gene="uspD"
                     /locus_tag="OQN13_001195"
     CDS             complement(13662..14090)
                     /gene="uspD"
                     /locus_tag="OQN13_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCS1614606.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14117..14416)
                     /locus_tag="OQN13_001196"
     CDS             complement(14117..14416)
                     /locus_tag="OQN13_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCS1614607.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14628..15068)
                     /locus_tag="OQN13_001197"
     CDS             complement(14628..15068)
                     /locus_tag="OQN13_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS1614608.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15169..15768
                     /locus_tag="OQN13_001198"
     CDS             15169..15768
                     /locus_tag="OQN13_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCS1614609.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNSEKGLTFAVEPIKLALSESLEGLNK"
     gene            15876..16643
                     /gene="tpiA"
                     /locus_tag="OQN13_001199"
     CDS             15876..16643
                     /gene="tpiA"
                     /locus_tag="OQN13_001199"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCS1614610.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16698..17453)
                     /gene="cdh"
                     /locus_tag="OQN13_001200"
     CDS             complement(16698..17453)
                     /gene="cdh"
                     /locus_tag="OQN13_001200"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCS1614611.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17560..18549)
                     /gene="sbp"
                     /locus_tag="OQN13_001201"
     CDS             complement(17560..18549)
                     /gene="sbp"
                     /locus_tag="OQN13_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCS1614612.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(18869..19831)
                     /gene="pfkA"
                     /locus_tag="OQN13_001202"
     CDS             complement(18869..19831)
                     /gene="pfkA"
                     /locus_tag="OQN13_001202"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCS1614613.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20012..20914)
                     /gene="fieF"
                     /locus_tag="OQN13_001203"
     CDS             complement(20012..20914)
                     /gene="fieF"
                     /locus_tag="OQN13_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCS1614614.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21062..21562)
                     /gene="cpxP"
                     /locus_tag="OQN13_001204"
     CDS             complement(21062..21562)
                     /gene="cpxP"
                     /locus_tag="OQN13_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCS1614615.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21712..22410
                     /gene="cpxR"
                     /locus_tag="OQN13_001205"
     CDS             21712..22410
                     /gene="cpxR"
                     /locus_tag="OQN13_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCS1614616.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22407..23780
                     /gene="cpxA"
                     /locus_tag="OQN13_001206"
     CDS             22407..23780
                     /gene="cpxA"
                     /locus_tag="OQN13_001206"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCS1614617.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(23886..24560)
                     /gene="yiiM"
                     /locus_tag="OQN13_001207"
     CDS             complement(23886..24560)
                     /gene="yiiM"
                     /locus_tag="OQN13_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCS1614618.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24709..25692)
                     /gene="kdgT"
                     /locus_tag="OQN13_001208"
     CDS             complement(24709..25692)
                     /gene="kdgT"
                     /locus_tag="OQN13_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCS1614619.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(25952..26572)
                     /gene="sodA"
                     /locus_tag="OQN13_001209"
     CDS             complement(25952..26572)
                     /gene="sodA"
                     /locus_tag="OQN13_001209"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCS1614620.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            26857..27891
                     /gene="rhaT"
                     /locus_tag="OQN13_001210"
     CDS             26857..27891
                     /gene="rhaT"
                     /locus_tag="OQN13_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCS1614621.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(27888..28736)
                     /gene="rhaR"
                     /locus_tag="OQN13_001211"
                     /pseudo
     CDS             complement(27888..28736)
                     /gene="rhaR"
                     /locus_tag="OQN13_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(28810..29646)
                     /gene="rhaS"
                     /locus_tag="OQN13_001212"
     CDS             complement(28810..29646)
                     /gene="rhaS"
                     /locus_tag="OQN13_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCS1614622.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            29934..31403
                     /gene="rhaB"
                     /locus_tag="OQN13_001213"
     CDS             29934..31403
                     /gene="rhaB"
                     /locus_tag="OQN13_001213"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCS1614623.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            31400..32659
                     /gene="rhaA"
                     /locus_tag="OQN13_001214"
     CDS             31400..32659
                     /gene="rhaA"
                     /locus_tag="OQN13_001214"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCS1614624.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQAIGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            32901..33725
                     /gene="rhaD"
                     /locus_tag="OQN13_001215"
     CDS             32901..33725
                     /gene="rhaD"
                     /locus_tag="OQN13_001215"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCS1614625.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33735..34049
                     /gene="rhaM"
                     /locus_tag="OQN13_001216"
     CDS             33735..34049
                     /gene="rhaM"
                     /locus_tag="OQN13_001216"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCS1614626.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            34350..34796
                     /locus_tag="OQN13_001217"
     CDS             34350..34796
                     /locus_tag="OQN13_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCS1614627.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            34807..>35431
                     /locus_tag="OQN13_001218"
     CDS             34807..>35431
                     /locus_tag="OQN13_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS subunit IIBC"
                     /protein_id="HCS1614628.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAP"
BASE COUNT         8673 a         9209 c         9179 g         8370 t
ORIGIN      
        1 gtaaaatcct tttgaaataa ataagaacca gaagcctagc gccagtcaac aacaggaaat
       61 gataatgagt taacaccccg gaatttttca gaatttataa aaggcaaatt acaggcacaa
      121 aaaaagcgcc gtgcggcgct tttttcggaa atccggtctt atttgctgcc cgggatgttg
      181 aaacgcttgt tgaagcggtc aacacggcca ccggtagcaa catcacgctg tttgccagtg
      241 aagaacgggt ggcacttgct gcacacgtcg aggttcaggt catgaccaac ggtggagcgg
      301 attttcatta cgttaccgca agagcagcta gcagtaattt cttcgtattt cgggtgaata
      361 tcttttttca tgggaaaacc tcggtttaag gccgcgtcgc tcttccagcc ctaacgccag
      421 acaccacgcg atgttaaaag tatagcttca atacgatcat ttcgtacgaa ggcgcgaaat
      481 catacagaaa ttaaccagcg tatgcaaact gatccgcact cttctacggc aatgtgtata
      541 ctaacccacc gaatttcaag tcaggatgat gctatgcccg ttgcccacgt tgccttgccc
      601 gttccgcttc ctcgtacctt tgactatctg ctgccagaag gcatggcggt taaagccggg
      661 tgtcgcgtgc gcgtgccgtt tggcaaacag caggagcgca tcggggttgt ggtatcagtt
      721 agcgatgtca gtgagctgcc gcttaacgag ctgaaagcgg tagttgaagt gctggacgtt
      781 gagccggtat ttactcactc cgtctggcga ttactgctct gggcggcaga ttactatcat
      841 catccgattg gcgatgtgct gtttcatgcc ttgccgattt tactgcgcca ggggcggcct
      901 gcggcgaacg cgccgatgtg gtactggttt gccactgaac aaggccaggc ggtggatctg
      961 aacagcctga aacgctcacc caagcaacaa caggcgctgg cggcgttacg gcaagggaaa
     1021 atctggcgcg accaggttgc cacgctcgaa tttaatgatg ccgcgttgca gacgctgcgc
     1081 aaaaaaggtc tgtgtgattt agcaagtgaa acaccagagt ttagcgactg gcgaacgaac
     1141 tatgccgttt ctggtgagcg gttgcgattg aataccgaac aggccaccgc cgttggcgca
     1201 attcatagcg cggcagatac tttttctgcc tggctgctgg cgggcgttac cggttccggt
     1261 aaaacggagg tttatctcag tgtactggaa aacgtgctcg ctcagggcaa acaggcgctg
     1321 gtgatggtgc cggaaatcgg cctgacaccg caaactatcg cccgttttcg tgaacggttt
     1381 aatgcgcccg tggaagttct gcattccggt ctgaacgaca gcgagcgtct ttcggcgtgg
     1441 ctgaaagcga aaaatggcga ggcggcgatt gtgatcggca cccgctccgc gctgtttacg
     1501 ccgtttaaaa atctcggcgt gattgtcatt gacgaagagc acgacagttc ctacaagcag
     1561 caggaaggct ggcgctatca tgcccgcgac ctggcggtgt atcgtgcgca tagcgagcaa
     1621 atcccgatta ttcttggctc cgcaacgccc gcgctggaaa cgttgtgcaa cgtccagcag
     1681 aaaaaatacc gcctgctgcg cctgacgcgc cgggcgggga atgcgcgtcc ggcaattcaa
     1741 cacgtgctgg atttaaaagg tcagaaggtg caggcaggtc tggctccggc gttaatcacc
     1801 cgtatgcgcc agcatttaca ggccaacaat caggttatcc tctttcttaa ccgccgtggc
     1861 tttgcgcctg cgctactgtg ccacgactgc ggctggattg ccgaatgccc acgttgcgat
     1921 cactactaca cgctgcatca ggcgcaacag catttgcgct gtcaccactg tgacagtcag
     1981 cgtccggtgc cgcgccagtg cccttcctgc ggttccacgc acctggtacc cgtggggctg
     2041 ggcaccgaac agcttgaaca gacgcttgcg ccgctgttcc ctgatgtgcc catttctcgt
     2101 atcgaccgcg ataccaccag ccgcaaaggg gcgctggaac agcaactggc ggaagtacat
     2161 cgcggcggtg cgaggatttt gatcggcaca caaatgctgg cgaaaggtca ccactttccg
     2221 gatgtgacgc tggtggcgtt actggatgtg gacggcgcgc tgttttctgc cgattttcgc
     2281 tcggcagagc gtttcgctca gctttacacc caggtcgccg gtcgtgccgg gcgtgcgggt
     2341 aaacagggcg aagtggtgct gcaaacgcac catccggaac atcctctgtt gcaaacgttg
     2401 ctctataaag gctacgacgc ctttgccgaa caggcgctgg ctgagcgacg catgatgcag
     2461 ctgccgccgt ggaccagcca tgtgattgtg cgtgcggaag atcataacaa tcagcatgcg
     2521 ccgctgttcc tgcaacaact gcgtaatctg atcctctcca gcccgctggc agacgacaaa
     2581 ttgtgggttc tcggtccggt tccggcactg gcacctaaac gtggcggtcg ctggcgctgg
     2641 cagatattgt tgcagcaccc ttcccgcgtg cgcttgcaac acatcattaa cggtacgctg
     2701 gcactcatca atacaatacc ggattcccgt aaggtgaaat gggtgctgga tgttgatccg
     2761 attgagggtt aaaccgctca cgatgcgagg cggatcgaaa aattcaatat tcatcacact
     2821 tttcatgaaa attctgtaac cgttttcacg cgctatctgc taaaaatgtt gccgatgtga
     2881 agtaaacatg gatgtagtac gcctgacgtg ccaggcgagg agtgagtgtg aaagcgaaga
     2941 agcaggaaac tgccgcgacc atgaaagacg ttgccctcaa ggcaaaagtc tctacagcga
     3001 ctgtctcccg agcattaatg aatcccgata aagtctccca ggccacccgt aatcgggttg
     3061 aaaaagcggc ccgggaagtg ggttatttac cgcagcctat ggggcgcaac gtcaagcgta
     3121 atgaatcccg caccattctg gtgattgtcc cggatatctg cgatcccttc tttagcgaaa
     3181 ttattcgcgg tatcgaagtt acggcggcaa atcacggata tctggtgctg attggcgact
     3241 gtgcgcatca aaatcagcag gaaaaaacct ttatcgattt gatcatcacc aagcaaattg
     3301 atggcatgtt gctgctgggt tcaaggctgc cgtttgatgc cagcattgag gaacagcgta
     3361 atctgccgcc gatggtgatg gcgaacgaat tcgcaccgga actggagcta cctacagttc
     3421 atatcgacaa tctgaccgcc gcatttgatg cagtaaatta tctatatgag caagggcata
     3481 aacggattgg ctgtatagcc ggtcccgaag agatgccgct gtgtcactac cgcctgcaag
     3541 gttacgttca ggcgctgcgt cgctgcggca ttatggttga tccgcaatac atcgcccgtg
     3601 gcgacttcac cttcgaagcc ggaagcaaag cgatgcagca gctgcttgat cttccacaac
     3661 cgcctactgc tgtcttctgc catagcgatg tgatggcgct cggcgcactt tctcaggcaa
     3721 aacgccaggg gctgaaagtc ccggaagacc tttccataat cggttttgat aacatcgacc
     3781 tgacgcaatt ttgtgatccg ccgctgacaa ccatcgcgca gccgcgttac gaaatcggtc
     3841 gggaagctat gctgttattg cttgatcaaa tgcaggggca acacgttggc agtggctctc
     3901 gtttaatgga ctgcgaactt atcatccggg gatcaacacg cgcgttacct taaagtaaac
     3961 ctttaagact gccgtgactg gtcaaaggcc cgccgcttaa gtaacatggc gggctgacga
     4021 acgaataaat acagcgaaac gatagtggca caacgagatt atgtacgccg cagccaaccg
     4081 gcaccttcgc ggcgaaaaaa gagcacctca cggaaaaagc aacgaaatct gcctgcggtt
     4141 tctcccgcta tggtcgctat tgctgccgcc gtgcttgtga cctttatcgg tggtctgtac
     4201 ttcattacgc atcacaagaa agaagagtcc gagacgctgc aaagccagaa agtgaccggt
     4261 aacggactac caccaaaacc agaagaacgc tggcgctaca ttaaagagct ggaaagtcgc
     4321 cagccgggtg tgcgtgcgcc cacagaacct tctgccggtg gtgaagtgaa aacgccggag
     4381 caactgacac cagaacaacg tcagcttctt gaacaaatgc aggctgatat gcgccagcag
     4441 ccaacgcagc tggttgaagt tccgtggaat gaacagacgc cggaacagcg tcagcaaacg
     4501 ctacaacgcc agcgtcaggc gcagcaatta gcggagcagc aacgcctggc tcagcagtcg
     4561 cgtacgactg aacaaagctg gcagcagcag acgcgtacgt cgcaagccgc gccggtacag
     4621 gcgcaaccgc gccagtctaa accggcttct actcagcagc cgtaccagga tctgctgcaa
     4681 acccctgcgc acacgaccgc gcagtcgaag ccgcagcaag ctgcgccggt tgctcgtgcc
     4741 gctgacgcgc caaaaccgac ggcggagaaa aaagacgaac gccgctggat ggtgcagtgc
     4801 ggttcgttca gaggcgcgga acaggcagag acggtacgtg ctcagctggc gttcgaagga
     4861 tttgactcga aaatcaccac caacaatggc tggaatcgtg tggtcattgg cccggtgaaa
     4921 ggcaaagaga acgcagacag caccctcaat cggttgaaga tggcgggtca tacaaactgc
     4981 attcggctcg ccgccggggg ttgaaaccct caaaatcccc cccatctata attgcattat
     5041 gccccgtact tttgtacggg gtttgtactc tgtattcgta accaaggggt cagctcgtga
     5101 caactatagt aagcgtacgc cgtaacggcc atgtggtcat cgctggtgat ggtcaggcca
     5161 cgttgggcaa taccgtaatg aaaggcaacg tgaaaaaggt ccgccgtctg tacaacgaca
     5221 aagtcatcgc gggctttgcg ggcggtactg cggatgcttt tacgctgttc gaactgtttg
     5281 aacgtaaact ggaaatgcat cagggccatc tggtcaaagc cgccgttgag ctggcaaaag
     5341 actggcgtac cgatcgcatg ctgcgcaaac ttgaagcact gctggcagtc gcggatgaaa
     5401 ctgcatcgct tatcatcacc ggtaacggtg acgtggtgca gccagaaaac gatcttattg
     5461 ctatcggctc cggcggccct tacgcccagg ctgcggcgcg cgcgctgtta gaaaacactg
     5521 aacttagcgc ccgtgaaatt gctgaaaagg cgttggatat tgcaggcgac atttgcatct
     5581 ataccaacca tttccacacc atcgaagaat taagctacaa agcgtaagga tctcccatgt
     5641 ctgaaatgac cccacgcgaa atcgtcagcg aactggataa gcacatcatc ggccaggaca
     5701 acgccaagcg ttctgtggcg attgctctgc gtaaccgctg gcgtcgcatg cagctcaacg
     5761 aagagctgcg ccatgaagtg accccgaaaa atatcctgat gatcggcccg accggtgtcg
     5821 gtaaaactga aatcgcccgt cgtctggcta agctggcgaa tgcgccgttc atcaaagttg
     5881 aagcgaccaa attcaccgaa gtgggctatg tcggtaagga agtggattct attattcgcg
     5941 atctgaccga tgccgccgtg aaaatggtac gcgtccaggc tatcgagaaa aaccgttatc
     6001 gcgctgaaga actggcagaa gaacgtattc tcgacgtgct gatcccacct gctaaaaaca
     6061 actggggaca gaccgaacag cagcaggaac cgtccgctgc tcgtcaggca ttccgcaaaa
     6121 aactgcgtga aggccagctt gatgacaaag aaatcgagat cgatcttgcc gcagcaccga
     6181 tgggcgttga aattatggct cctccaggca tggaagagat gaccagccag ctgcagtcca
     6241 tgttccagaa cctgggcggc cagaagcaaa aagcgcgtaa gctgaaaatc aaagacgcca
     6301 tgaagctgct gattgaagaa gaagcggcga aactggtgaa cccggaagag ctgaagcaag
     6361 acgctatcga cgctgttgag cagcacggga tcgtgtttat cgacgaaatc gacaaaatct
     6421 gtaagcgcgg cgagtcttcc ggtccggatg tttctcgtga aggcgttcag cgtgacctgc
     6481 tgccgctggt agaaggttgc accgtttcca ccaaacacgg gatggtcaaa actgaccaca
     6541 ttctgtttat cgcttctggc gcgttccaga ttgcgaaacc gtctgacctg atcccggaac
     6601 tgcaaggtcg gctgccaatc cgcgttgaac tgcaggcact gaccaccagc gacttcgagc
     6661 gtattctgac cgagccgaat gcctctatca ccgtgcagta caaagcactg atggcgactg
     6721 aaggcgtaaa tatcgagttt accgactccg gtattaagcg catcgcggaa gcggcatggc
     6781 aggtgaacga atctaccgaa aacatcggtg ctcgtcgttt acacactgtt ctggagcgtt
     6841 taatggaaga gatttcctac gacgccagcg atttaagcgg tcaaaatatc actattgacg
     6901 cagattatgt gagcaaacat ctggatgcgt tggtggcaga tgaagatctg agccgtttta
     6961 tcctataatc gcgttcaatc attttcatca ttgtttgatg gggctgaaag gccccatttt
     7021 tattggcgcg tattatgact gaacaacaaa ttagccgaac tcaggcgtgg ctggaaagtt
     7081 tacgacctaa aaccctcccc ctcgcctttg ctgcaattat cgtcgggacg gcgctggcat
     7141 ggtggcaagg tcacttcgat ccgctggtcg ccctgctggt actaattacc gccgggctat
     7201 tacagatcct ttctaacctc gccaatgatt acggcgatgc ggtaaaaggc agcgataaac
     7261 ctgaccgcat tgggccgcta cgcggcatgc aaaaaggggt cattacccag caagagatga
     7321 aacgggcgct cattattacc gtcgtgctca tctgtctctc cgggctggca ctggttgcag
     7381 tggcatgcca tacgctggcc gattttgtcg gtttcctgat tcttggcggg ttgtcgatca
     7441 ttgccgctat cacctacacc gtgggcaatc gtccttatgg ttatatcggt ctgggtgata
     7501 tttccgtact ggttttcttt ggctggttga gcgtcatggg gagctggtat ttacaggctc
     7561 atacattgat tccggcactg atccttccgg cgaccgcgtg tggcctgctg gcaacggcag
     7621 tactgaacat taataacctg cgtgatatca atagcgaccg cgaaaatggc aaaaacacgc
     7681 tggtggtgcg cttaggtgaa gtgaacgcgc gtcgttatca tgcctgcctg ctgatgggct
     7741 ctctggtatg tctggcgctg tttaatctct tttcgctgca tagcctgtgg ggctggctgt
     7801 tcctgctggc ggcaccatta ctggtgaagc aagcccgtta tgtgatgcgg gaaatggacc
     7861 cggtggcgat gcgaccaatg ctggaacgta ctgtcaaggg agcgttactg actaacctgc
     7921 tgtttgtttt agggatattc ctaagccagt gggcagcata actgacaaat atcaattaac
     7981 aattgatgat tttgccaaca gcctacatag cgcgatatac tgaaaattct cgcagcaact
     8041 gaatgttaag cctatgaaat acgatacttc cgagctttgt gacatctatc aagaagatgt
     8101 taacgtcgtg gaaccgctgt tctccaactt tggcggacgg gcgtcgtttg gcggacaaat
     8161 aatcacggta aaatgtttcg aggacaacgg gttgctgtac gatctgctcg aacagaatgg
     8221 ccgtggtcgt gttcttgtcg tcgatggcgg tggttctgtt cgtcgcgcac tggtcgatgc
     8281 tgaactggcg cgtctggcag tacaaaatga atgggaaggt ctggttattt acggcgcggt
     8341 gcgtcaggta gatgacctag aagagttgga tatcggcatc caggcgatgg cggcaattcc
     8401 ggttggtgcc gctggcgaag gcattggcga aagcgatgtc cgcgtcaatt ttggcggtgt
     8461 aaccttcttc tccggcgacc atctttatgc cgacaatacc gggattattc tttcagaaga
     8521 tccgctggat attgaatgat aagaaaggca ccgcaaggtg ccttttttct gcgttacctg
     8581 ttggcctaca cagtaaagaa attacgcgaa agatgaagcg taatcagacc tcttccatgc
     8641 gacccagcag ggcctgcaga cgttcctgcc agccgttctg ctgttctttc agatggttgt
     8701 tctcacgctc cagctcttcg cgctgatgct gggcattttg aacttcctgc gacagtgagt
     8761 tgtttttttc tttcagctct tcgatttcca tctgcaacag agtgatggta tcaatcgcct
     8821 gctgtacttt tgcttccagt ttctcaaaca cttctaatga cattgtcata cctctcctga
     8881 attgcaaggc gttgatggat aaaaaatcct cgtcccgatt accggtgacg ccttaataaa
     8941 tacgagcgca ctttagttag ctccgattgt atgaagccgc gccatcgctg tccagcggca
     9001 cgccttgcag attacggttt gccacacttt tcatccttct cctggtgaca taatccacat
     9061 caatcgaaaa tgttaataaa tttgttgcgc gaatgatcta acaaacatgc atcatgtaca
     9121 atcagatgga ataaattgcg cgataacgct cattttatga cgaagcacac acattttaag
     9181 ttcgatattt ctcgtttttg ctcgttaacg ataagtttac agcatgccta caagcatcgt
     9241 ggaggtccgt gactttcacg catacaacaa acattaactc ttcaggatcc gattatgagt
     9301 caaacatcaa ccttgaaagg ccagtgcatt gctgaattcc tcggtaccgg gttgttgatt
     9361 tttttcggtg tgggttgcgt tgcagcacta aaagtcgctg gtgcgtcttt tggtcagtgg
     9421 gaaatcagtg tcatttgggg actgggggtg gcaatggcca tctacctgac cgcaggggtt
     9481 tccggcgcgc atcttaatcc cgctgttacc attgcattgt ggctgtttgc ctgtttcgac
     9541 aagcgcaaag ttattccttt tatcgtttca caagttgccg gcgctttctg tgctgcggct
     9601 ttagtttacg ggctttacta caatttattt ttcgacttcg agcagactca tcacattgtt
     9661 cgcggcagtg ttgaaagtgt tgatctggca ggcactttct ctacttaccc taatcctcat
     9721 atcaattttg tgcaggcttt cgcagttgag atggtgatta ccgctattct gatggggctg
     9781 atcctggcgt taacggacga tggcaacggt gtaccacgcg gccctttggc tcccttgctg
     9841 attggtctac tgattgcggt cattggcgca tctatgggcc cattgacggg ttttgccatg
     9901 aacccagcgc gtgacttcgg tccgaaagtc tttgcctggc tggcgggctg gggcaatgtc
     9961 gcctttaccg gcggcagaga cattccttac ttcctggtgc cgctttttgg ccctatcgtt
    10021 ggcgcgattg taggtgcatt tgcctaccgc aaactgattg gtcgccattt gccttgcgat
    10081 atctgtgttg tggaagaaaa ggagaccaca actccttcag aacaaaaagc ttcgctgtaa
    10141 tatgactacg ggacaattaa acatgactga aaaaaaatat atcgttgcgc tcgaccaggg
    10201 caccaccagc tcccgcgcgg tcgtaatgga tcacgatgcc aatatcatta gcgtgtcgca
    10261 gcgcgaattt gagcaaatct acccaaaacc aggctgggta gaacacgacc caatggaaat
    10321 ctgggccacc caaagctcca cgctggtaga agtgctggcg aaagccgata tcagttccga
    10381 tcaaattgca gctatcggta ttacgaacca gcgtgaaacc actattgtct gggaaaaaga
    10441 aaccggcaag cctatctata acgccattgt ctggcagtgc cgtcgtaccg cagaaatctg
    10501 cgagcattta aaacgtgacg gtttagaaga ttatatccgc agcaataccg gtctggtgat
    10561 tgacccgtac ttttctggca ccaaagtgaa gtggatcctc gaccatgtgg aaggctctcg
    10621 cgagcgtgcg cgtcgtggtg aattgctgtt tggtacggtt gatacgtggc ttatctggaa
    10681 aatgactcag ggccgtgtcc atgtgaccga ttacaccaac gcctctcgta ccatgttgtt
    10741 caacatccat accctggact gggacgacaa aatgctggaa gtgctggata ttccgcgcga
    10801 gatgctgcca gaagtgcgtc gttcttccga agtgtacggt cagactaaca ttggcggcaa
    10861 aggcggcacg cgtattccaa tctccgggat cgccggtgac cagcaggccg cgctgtttgg
    10921 tcagttgtgc gtgaaagaag ggatggcgaa gaacacctat ggcactggct gctttatgct
    10981 gatgaacacc ggcgagaaag cggtgaaatc agaaaacggc ctgctgacca ccatcgcctg
    11041 cggcccgact ggcgaagtga actatgcgct ggaaggtgcg gtgtttatgg caggcgcatc
    11101 cattcagtgg ctgcgcgatg agatgaagtt gattaacgac gcctacgatt ccgaatattt
    11161 cgccaccaaa gtgcaaaaca ccaatggtgt gtatgtggtt ccggccttta ccgggctggg
    11221 tgcgccgtac tgggacccgt atgcgcgcgg ggcgattttc ggtctgactc gtggggtgaa
    11281 cgctaaccac attatacgcg cgacgctgga gtctattgct tatcagacgc gtgacgtgct
    11341 ggaagcgatg caggccgact ctggtatccg tctgcacgcc ctgcgcgtgg atggtggcgc
    11401 agtagcaaac aatttcctga tgcagttcca gtccgatatt ctcggcaccc gcgttgagcg
    11461 cccggaagtt cgcgaagtga ctgcattggg cgcggcctat cttgctggtc tggcggtcgg
    11521 tttctggcag aacctcgacg agctgcaaga gaaagcggtg attgagcgcg agttccgtcc
    11581 aggcatcgaa accactgagc gtaattaccg ttacgcaggc tggaaaaaag cggttaaacg
    11641 cgcgatggcg tgggaagaac acgacgtaat gtaaatgccg gatgaagcgt ttatgccgca
    11701 tccggtagtc ccgaaacgtg cgggggcaac cccgcacaca tcaataatcc ctcccttccc
    11761 ctgtgctaca cttcgcgcca ttccttactg cttagagttt gctatgagac gagaacttgc
    11821 catcgaattt tcccgcgtca ccgaatctgc ggcgctggct ggctacaaat ggttaggacg
    11881 cggcgataaa aacaccgcgg acggcgcggc ggtaaacgcc atgcgtatta tgctcaacca
    11941 ggtcaatatt gacggcacca tcgtcattgg tgaaggtgaa atcgacgaag caccgatgct
    12001 ctacattggt gaaaaagtcg gtactggtcg cggcgacgcg gtagatattg ctgttgatcc
    12061 gattgaaggc acgcgcatga cggcgatggg ccaggctaac gcgctggcgg tgctggcagt
    12121 aggcgataaa ggctgcttcc tcaatgcgcc ggatatgtat atggagaagc tgatcgtcgg
    12181 accgggagcc aaaggcacca ttgatttgaa cctgccgctg gcggataacc tgcgcaatgt
    12241 tgcggcggcg ctcggcaaac cgttgagcga actgacggta acaattctgg ctaaaccacg
    12301 ccacgatgcc gttatcgctg aaatgcaaca actcggcgta cgcgtatttg ctattccgga
    12361 tggcgacgtt gcggcctcaa ttctcacctg tatgccagac agcgaagttg acgtgctgta
    12421 cggtattggt ggcgcgccgg aaggcgtagt ttctgcggcg gtgatccgcg cattagatgg
    12481 cgacatgaac ggtcgtctgc tggcgcgtca tgacgtcaaa ggcgacaacg aagagaatcg
    12541 tcgcattggc gagcaggagc tggcacgctg caaagcgatg ggcatcgaag ccggtaaagt
    12601 attgcgcctg ggcgatatgg cgcgcagcga taacgtcatc ttctctgcca ccggtattac
    12661 caaaggcgat ctgctggaag gcattagccg caaaggcaat atcgcgacta ccgaaacgct
    12721 gctgatccgc ggcaagtcac gcaccattcg ccgcattcag tctatccact atctggatcg
    12781 caaggacccg gaaatgcagg tgcacatcct ctgattgatt tgatcgattg agccttccag
    12841 tccttcggga ctggaatttt tttgttcgga gaacgaagat aaggcaagtc aatcaaaaca
    12901 ggagaaaaac atggctgatt gggtaacagg caaagtcact aaagtgcaga actggaccga
    12961 cgccctgttt agtctcaccg ttcacgcccc cgtgcttccg tttaccgccg ggcaattcac
    13021 caagcttggc cttgaaatcg acggcgaacg cgtccagcgc gcctactcct atgttaactc
    13081 gcccgataat cccgatctgg agttttacct ggtcaccgtc cccgatggca aattaagccc
    13141 acgactggcg gcactgaaac caggcgatga agtgcaggtg gttagcgaag cagctggctt
    13201 ctttgttctg gaggaagtac cagattgcga aacgctatgg atgctggcaa ccggtacagc
    13261 gattggccct tatttatcga ttctgcaact aggcaaagat ttagatcgct tcaaaaatct
    13321 ggtcctggtt cacgccgcac gttatgccgc cgacttaagc tatttgccac tgatgcaaga
    13381 actggaaaaa cgctacgaag gcaaactgcg cattcagacg gtggtcagtc gggaaacggc
    13441 agcggggtcg ctcaccggac ggataccggc attaattgaa agtggggaac tggaaagcgc
    13501 gattggcctg ccgatgaata aagaaaccag ccatgtgatg ctgtgcggca atccacagat
    13561 ggtgcgcgat acacaacagt tgctgaaaga gacccggcag atgacgaaac atttacgtcg
    13621 ccgaccgggc catatgacag cggagcatta ctggtaagcg gttacttatc gataaacggc
    13681 acgatgagca aatccgcact catcttattg atcatcccgc gatatgccgg catcaaacgg
    13741 ttgataaatg agtggtgatg accacagaca aggaggtcgc actgctcttt ttgcataatt
    13801 tccagcagtg tttccggcat ttctccgcgt tcaatacgca gttttgtctt cggccattga
    13861 atatttttcg tcagtttata cagcttgtta tccgacttat tcttcaacaa ttgaagaata
    13921 tcttctgttg cagggaagta gatacccgga tacaactcgc ttaagccatc atcaatatga
    13981 attaacgtca ggtgagcgtc attatgtctg gcgagctcca gggctttatt caccagtaag
    14041 gcatcttctt cattcccgga aattgccacg ccaatgtgtt tataagccat gtttaactcc
    14101 ttctaaagca actccatcaa gctaacgaac gcagtgataa ctcaaaaata atcatctcgg
    14161 cctggcatga gaaagtgaag gccgcatcaa gctcaacttg cccctccaga tccttaatct
    14221 tataggccac gctggcccct tcatctgcag aggatgcagc ggcggcaagc tctttcagtt
    14281 tggtcataaa agactctgct tcttcgcggg tggcaaaaaa gcgcgaaaac ttactggtac
    14341 agttgtcgtt atcaatcacc gtaccgatat caatcgcaca tcctttagta ctgcatttat
    14401 ctacgacatc tttcataggg cacctctgta tattcgcctc tctgtcgatt acgacttaag
    14461 aataatgcgc cgttaaggga agaacaaaca agtaatgatc ttatggatta ccattttttt
    14521 tgatattgct taaggtgaag agttcagaag gattttccgg gtattgtcat ttgcatcgtg
    14581 gaaataacaa tcccggaaaa aaatagcagt aagagatata ttaaaggtta attacttgat
    14641 ttattgtcgg ctttatactt cacatcctga gtatctttac catatttatt ttcgccttgt
    14701 gtgccaacaa acgcaccaag atcgataagc atcatcacca gaatcaacgt cgggacaaaa
    14761 cgccccaccg cccattgcca gacacccggt aaaatcgccc agttacccgc cagcagcatc
    14821 cacgccacaa tcatcagaaa tgcccatgcg ccggaacgcc cgcgatcatg caagcgctta
    14881 acagttactg ccgctgttgg ccaaagcaag cacacaaggc aaaacgccgc ggtctgaata
    14941 tcgagtaaat ttttacccgc cagtgagaaa agcaccagca tgcctgcgaa ccacaggcct
    15001 atccaaatcc agaaatcacg gcgtccaata cgccctttaa atgagaataa ccattgctgt
    15061 atggtcatgt aagttccttg atggttgtct tttccaggat tctacccttt tgacaagggc
    15121 gacagatatc gttttaatcg gagccagtca taacaaaagg tactgtcaat gaagccaggg
    15181 tgtacgctgt tttttctctt atgttctgca ttaaccgtta caacaacggc gcatgcacaa
    15241 acaccagata cggcaacgac cgcgccttat ctgctggctg gagcccctac tttcgatctc
    15301 tccatcagcc agtttcgaga agactttaac agccagaatc ctagcctgcc actgaacgaa
    15361 tttcgtgcca tcgacagcag tcccgacaaa gccaatctca ctcgtgctgc aagtaaaatt
    15421 aacgagaact tgtatgcttc tacagcgctg gagcgcggta ccttaaaaat caaaagcatt
    15481 caaatgacct ggctacccat ccaggggcca gagcaaaaag cagcgaaagc gaaagctcag
    15541 gaatacatgg cagcagtgat ccgcacactc accccattaa tgaccaaaac acaaagccag
    15601 aaaaaactgc agtcgctact aacggcgggg aaaaacaaac gttattacac cgagacagaa
    15661 ggtgcactgc gttatgttgt cgcggacaac agcgaaaagg ggctgacctt cgctgttgaa
    15721 ccgattaagc tggcgctatc tgaatcgctt gaaggtttga ataaatgaca aaaagcaaag
    15781 cctttgtgcc gatgaatctc tatactgttt cacagacctg ctgccctgcg gggcggccat
    15841 cttcctttat tcgcttataa gcgtggagaa ttaaaatgcg acatccttta gtgatgggta
    15901 actggaaact gaacggcagc cgccacatgg ttcacgagct ggtttctaac ctgcgtaaag
    15961 agctggcagg tgttgctggc tgtgcggttg caatcgcacc accggaaatg tacatcgata
    16021 tggcgaagcg cgaagctgaa ggcagccaca tcatgctggg tgcgcaaaac gtggacctga
    16081 acctgtccgg cgcattcacc ggtgaaactt ctgccgctat gctgaaagac atcggcgctc
    16141 agtacatcat catcggccac tctgaacgtc gtacttacca caaagagtct gacgaactga
    16201 tcgcgaaaaa attcgcggtg ctgaaagagc agggcctgac tccggttctg tgcatcggtg
    16261 aaaccgaagc tgaaaacgaa gcgggcaaaa ctgaagaagt ttgcgcacgt cagatcgacg
    16321 cggtactgaa aactcagggt gctgcggcat tcgaaggtgc ggttatcgct tacgaacctg
    16381 tatgggcaat cggtactggc aaatctgcaa ctccggctca ggcacaggct gttcacaaat
    16441 tcatccgtga ccacatcgct aaagttgacg ctaacatcgc tgaacaagtg atcattcagt
    16501 acggcggctc tgtaaacgcg tctaacgctg cagaactgtt tgctcagcca gatatcgacg
    16561 gcgcgctggt tggcggtgct tctctgaaag ctgacgcttt cgcagtgatc gttaaagctg
    16621 cagaagcggc taaacaggct taagtctgac aggtgccgga tttcatatcc ggcacttact
    16681 ttccttaact cttcgcctta acgcaaaatc tcacactgat gatcctgaat ttcctcggct
    16741 gaagcacggt taagcgtcag tagatttcgt tgtgtcgcca gcaatacaaa tgagttatca
    16801 ctctgccgta ccatcgccag cccgtagctt cccatatgtt cccgcgcctc aggtacttct
    16861 tctgccagca tcataaatgg gctgcgttgt accagttcgc tttccgttac ccgacgcgcc
    16921 aggtattcat gcccacgcaa accacctggc agtggcaacc agcggctgct gatgttcgcc
    16981 agattgttat ccagttgttc gcgcacatca ggacgaatac aagagatatg aatatgaaaa
    17041 tggttttgcg tacgcccggt gcgggagttg atcgccaaag aaaccgcgcg atcgggaacc
    17101 ggctggccgt attttttgct cataaaatca cgcgcctgcc aggccaacca aaagaagttc
    17161 ggcgttgaag gatcggtcaa caaaggactt tcagtaccgt taatacgata cgttggcatc
    17221 aacagatatt gcagtggacc attaagatct tttaaaacca cgtatccggc attgggtttg
    17281 acttccgcac atggcgaagg attttgattt tgctgctgat tgggcaaaca ttcctcaagg
    17341 acaatcttac gtaatgtatc cgactcttca ccggttaatt tccagtaacc aataccggca
    17401 gccacaacgg cgataactat catcaccaaa aaaagaagac ccgctttttt catctttttt
    17461 tccctgtacc tcaaagagat gcaagggtaa cgcaaaatcg tgacaaataa aaaacccggt
    17521 cgcgagaaac caccgggttt gataattatc ctggggaaat cagcgtttgc tgatctgatc
    17581 gaacgtaccg ccgttagcaa aatgctcttt ttgcgctttc gtccagccgc cgaactcttc
    17641 atcaatggtg aataacttca gctttggaaa cgcattttcg tactttttcg ctacttcagc
    17701 gtcgcgcgga cggtagtagt ttttcgcggc aatttcctga ccttctggcg agtagagata
    17761 tttcaggtag gcttccgcca cctctttggt gccttttttc tcgaccactt tatcgaccac
    17821 cgacacggtt ggctctgcga ggatagactc actcggcgtg acgatttcga atttatcttt
    17881 ccccagttca ttcgctgcca gcagagcttc gttttcccag gcgatcagta catcgccaat
    17941 cccgcgctcg acaaaggtgt tagtggagcc gcgcgcgcca gaatccagaa cttcgacgtt
    18001 tttatacagt gcccgaacaa aatcctgtgc ttttgcctga tcgttgttgt tgtgatgcag
    18061 cgcgtagccc caggcagcta ggtagttcca gcgtgcgcca ccagagcttt tcggattagg
    18121 cgtgatcacc gaaacacccg gtttaatcag atcgttccag tcatggatct gcttcggatt
    18181 gcccttacgc accaggaaaa caatggtgga agtgtacggt gcggagttat ccggcagacg
    18241 tttgatccac tctttatcaa tccgcccacg ttccgcaatc gcgtccacgt cataggccag
    18301 agccagcgtg acaacatcag cttcaatacc gttgattacc gacgtcgctt gtttacctga
    18361 accaccgtgt gactgacgaa tcaccacgtt atcaccagtt tgctgtttcc agtgggcgct
    18421 gaatgccttg ttgtactgtt cgtacaattc gcgcgttgga tcatatgaaa cgttaagaag
    18481 ctgaatatcc tttgccataa cgctggttgc cgccagcaaa aatgttaacc ctacgcccca
    18541 cttgttcatc gcccgactct cttatgttgt gttgtgatga gcaaagcgtg ccagaaggtt
    18601 aaccaaacat taaagaataa aaaaagattg gctataactt gcgggtatat gttgagggat
    18661 taaaaaggcg gaagaaatcc gcctcatatt gctgacaaag tgcgctttgt ccatgccgga
    18721 tgcggcgcga acgccttatc cggcctacaa aagtttgcaa attcaataaa ttgcagaatt
    18781 catgtaggcc tgataagcga agcgcatcag gcatttttgc ttctgtcatc ggtttcaggc
    18841 taaaggaatc tgcctttttc cgaaatcatt aatacagttt tttcgcgcag tccagccagt
    18901 cacctttgaa cggacgcttc atgttttcga tagcgtcgat gatgtcgtgg tgaaccagct
    18961 gttcgttctg gatacctaca caacgaccgc cgtaacctgc cagcagcaga tcgatagcgt
    19021 aagcgcccat acgggaagcc agaatacggt cgtaaggcac cggagaacca ccgcgctgga
    19081 tgtggcccag cacagttgcg cgggtttcac gaccggtttc tttctcgatg aaatgcgcca
    19141 gttcgtcaac atcacacata tgttcggtaa tcgccacgat cgcgtgtttt ttacctttcg
    19201 cgatacccgc tttgatttcg tttaccaggt cttcacggct gaattcaact tccggaacca
    19261 caacgaattc acagccaccg gcaatggccg cagccaacgt cagatcgcca caataacggc
    19321 ccatcacttc caccacggaa atacgctggt gagaagaaga ggtgtcacgc agacggtcga
    19381 tcgcttctac aacggtgctc agcgcagtga agaaaccgat agtgtagtca gtgcctttga
    19441 tgtcgttgtc gatagtgccc ggcagaccga tgcacgggaa gcccatttcg gtcagacgca
    19501 ttgcacccat gtaggaaccg tcaccgccga taaccaccag cgcgtcgata ccacgttttt
    19561 tcaggttttc gatagccacg gcgcggatgt tctcgtcgcg gaattccggg aagcgcgcag
    19621 aaccgaggaa cgtaccgcca cggttgatca tgtcagaaac gctgtaacgg tctagctgta
    19681 ccatacggtc ttcatacaga cccagatagc cgtcataaat gcccattact tccagacctt
    19741 ctgtcagcgc agaacgaaca accccgcgaa ttgcggcgtt catgcctggc gcatcaccgc
    19801 cgcttgtcaa cacaccgatt ttcttaatca tgactacctc tgaactttgg aatgcaaaat
    19861 aaaatctgtt gccggaagtc ttcttgcaca tcgaagtgat ccaacgaatg tgcaaatagt
    19921 ataacaatca cttcctgctg aattgattca ggtcaggcca aatggcggta ttttatacac
    19981 aaaatgcggg tctggctctc ttttatactg attatgaaag catagaccgt ttaccctccc
    20041 tgggtacgac ggaacagggg tcctgatgga taattacatc cgatcccgga aaacgccgta
    20101 aaatagcctg ctctacctga tccgccacca tatgtgcctg aaccaaaggc agagagtctt
    20161 ccatttccaa atgaatctga ataaagcggg tcggccctga ctgccgcgtg cgaagatcgt
    20221 gagcgccgct aacacccggc caggaagtca cgatatcaat aatttcttgc cgttcctcat
    20281 caggcaatgc gcgatccagt aatgactgta ccgcctcata tcccatgcgt aacgcgctat
    20341 ataaaatata gatgccgatt cccaatgcaa acagagcatc ggcgcgatgc cagccgtacc
    20401 aggacaaccc cagcgccagc agaattgcgc cgttcatcat aacatcagac tggtaatgta
    20461 gcatatcagc ccgcaccgcc tggctttgcg tccggcgcac cacccaccgc tgaaacgaga
    20521 caaggataat cgtacaaatt agcgccacaa ttgtcacgat aaccccgacg cctggatctg
    20581 tcatcggtgt tggagatacc agatgttgaa tacccgtcaa aaacaggaat agtgccgaac
    20641 cggagataaa catactttgc gccagcgccg cgagggactc agctttaccg tgaccaaacg
    20701 agtgattatc gtcggcaggt tgcagggaat atcgcaccac cagtaaattc gtcaacgacg
    20761 cgccgatatc caccagcgaa tccaccagcg cggcgagaat actcaccgac ccggtatacc
    20821 accatgcaaa aattttaatc agcaatagca gcgaagccat cgccgtcgca gcaatcgccg
    20881 cccgactgac cagccgtcca taagattgat tcataaatac tcccactatc aactgacgct
    20941 agtataacgg aagcaaatca tctgcaatgc attaagcagc aggcaaattg aggataaaaa
    21001 aaaccccaca tcatgtgggg gaagacaggg atggtgtcta tggcaaggaa aacagggttt
    21061 actactggga acgtgagttg ctactactca atagcttcaa cgatgaactt ttttgccatt
    21121 gcgtcacgtc acgcaactgc tccattcgtt gttgatgttt ctcgtttaaa accgcttgct
    21181 gctccggcgt taacaggcga tacatttggt tgcggacttt tgccatctca acctgacgag
    21241 caatttgctc attcgccatt ttttctgcct gtgcgcgcac agcgttttca tcaaaatttt
    21301 ctgcggtgac aaggcgatgc attgtctcca gttcgctaac attaacagga ggctgttcgt
    21361 gccgggcctg ttgcataaga tctcgcatct gctgacgctg atgttcggtt aaacttatgc
    21421 cgtcgaacat atggctctgc gtactgcgct gcgtaagttc ttcacccgga tgccagttat
    21481 cgcctgaacc gacttcagca gcgtggctta atgaactgac tgccagcgtt gaggccatga
    21541 cggcagcggt aactatgcgc atcatttgct cccaaaatct ttctgtcgcg attcaacgat
    21601 agagagttta cgattcaggc tgcaaacatg cgtcaggggg tgtaaaacaa cgtaaagtca
    21661 tggattagcg acgtctgatg acgtaatttc tgcctcggag gtatttaaac aatgaataaa
    21721 atcctgttag ttgatgatga ccgagagctg acttccctat taaaggagct gctcgagatg
    21781 gaaggcttca acgtgattgt tgcccacgat ggggaacagg cgcttgatct tctggacgac
    21841 agcattgatt tacttttgct tgacgtaatg atgccgaaga aaaatggtat cgacacatta
    21901 aaagcacttc gccagacaca ccagacgcct gtcattatgt tgacggcgcg cggcagtgaa
    21961 cttgatcgcg ttctcggcct tgagctgggc gcagatgact atctcccgaa accgtttaat
    22021 gatcgtgagc tggtggcacg tattcgcgcg atcctgcgcc gttcgcactg gagcgagcaa
    22081 cagcaaaaca acgacaacgg ttcaccgaca ctggaagttg atgccttagt gctgaatcca
    22141 ggccgtcagg aagccagctt cgacgggcaa acgctggagt taaccggtac tgagtttacc
    22201 ctgctctatt tgctggcaca gcatctgggt caggtggttt cccgtgaaca tttaagccag
    22261 gaagtgttgg gcaaacgcct gacgcctttc gaccgcgcta ttgatatgca catttccaac
    22321 ctgcgtcgta aactgccgga tcgtaaagat ggtcacccgt ggtttaaaac cttgcgtggt
    22381 cgcggctatc tgatggtttc tgcttcatga taggcagctt aaccgcgcgc atcttcgcca
    22441 tcttctggct gacgctggcg ctggtgttga tgttggtttt gatgttaccc aagctcgatt
    22501 cacgccagat gaccgagctt ctggatagcg aacagcgtca ggggctgatg attgagcagc
    22561 atgtcgaagc ggagctggcg aacgatccgc ccaacgattt aatgtggtgg cggcgtctgt
    22621 tccgggcgat tgataagtgg gcaccgccag gacagcgttt gttattggtg accaccgaag
    22681 gccgcgtgat cggcgctgaa cgcagcgaaa tgcagatcat tcgtaacttt attggtcagg
    22741 ccgataacgc cgatcatccg cagaagaaaa agtatggccg cgtggaactg gtcggtccgt
    22801 tctccgtgcg tgatggcgaa gataattacc aactttatct gattcgtccg gccagcagtt
    22861 ctcaatccga tttcattaac ttactgtttg accgcccgct attactgctg attgtcacca
    22921 tgttggtcag tacgccgctg ctgttgtggt tggcctggag tctggcaaaa ccggcgcgta
    22981 agctgaaaaa cgctgccgat gaagttgccc agggaaactt acgccagcac ccggaactgg
    23041 aagcggggcc acaggaattc cttgccgcag gtgccagttt taaccagatg gtcaccgcgc
    23101 tggagcgcat gatgacctct cagcagcgtc tgctttctga tatctctcac gagctgcgca
    23161 ccccgctgac gcgtctgcaa ctgggtacgg cgttactgcg ccgtcgtagc ggtgaaagca
    23221 aggaactcga gcgtattgaa accgaagcgc aacgtctgga cagcatgatc aacgatctgt
    23281 tggtgatgtc acgtaatcag caaaaaaacg cgctggttag cgaaaccatc aaagccaacc
    23341 agttgtggag tgaagtgctg gataacgcgg cgttcgaagc cgagcaaatg ggcaagtcgt
    23401 tgacagttaa cttcccgcct gggccgtggc cgctgtacgg caatccgaac gccctggaaa
    23461 gtgcgctgga aaacattgtt cgtaatgctc tgcgttattc ccatacgaag attgaagtgg
    23521 gctttgcggt agataaagac ggtatcacca ttacggtgga cgacgatggt cctggcgtta
    23581 gcccggaaga tcgcgaacag attttccgtc cgttctatcg gaccgatgaa gcacgcgatc
    23641 gtgaatctgg cggtacagga ttggggctgg cgattgttga aaccgccatt cagcagcatc
    23701 gtggctgggt gaaggcagaa gacagcccgc tgggcggttt acggctggtg atttggttgc
    23761 cgctgtataa gcggagttaa actccgcatt tgtaggcagg ataaggcgtt tacgccgcat
    23821 ccggcatttg agcaggatgc ctgatgcgac gctgatagcg tcttatcagg cctacactcc
    23881 gggggttatt ttccccacaa ccggcgggaa aaatcttcga tcttgccgct cagtcggcgc
    23941 ttttgcatcg tccgcgtcca gcttttcgat aacccggcgg cggagagtaa acggtgatac
    24001 tgatcgtcat caaacggcat atgccatgcg atggcggcag cctcctgcac ggtcacatcg
    24061 ctgacacggg aaaccaactc cagcggcgcg tccgcagaca ctttccctgg cgctatcacg
    24121 ctatacaacc accccacctt acctgtgttt tgcatcaact gcgctatatc gctgatatca
    24181 aaatggtaat tgagtttata gcaaggcgag cgcggctggc tgacctgaat taatgcctct
    24241 ccccagcgga aaatatcgcc catatgaaca ttactttccg tgagaccgtc ggttgagaga
    24301 ttttcgccaa acgcaggcgc aacaaacaac tccgcctgtt ccggaaattc ccgcacccag
    24361 tagagatagt gctcacgagg ataatgacac agcgctctgt ctggcccgcc gtgaaccttt
    24421 ttctccgcct gctcgtcacc ttccagcccc agctccgtca gcatcaactc accatcaacc
    24481 tggattttgg caattgcgct gggtttgcct tcgggataag cctgaatctt gcctgtgtat
    24541 acatcaaccg gatatcgcat ctgttccctc tccacgagaa atttccccat tagaccatat
    24601 ttattttatg tcaggcgtgc tgttttcgcg gtaagtatcc aggcaggaac tcaatagcaa
    24661 accggacgaa cgttagttca tccggctgtg gggattaagg aagcaggttt atttcaccgt
    24721 acctaaaatt tcgattttcg ctgctctggc tttgacttta cgtgaccaga tagaggtaag
    24781 aattggcacc agaatcgaag tcacaatgac cgccgtcgct accagcgaag ttgctgccgg
    24841 agccatcggt ttaaacgcag gcaccatttc tgcaatcagc acaggtgtcg ctaccgcggc
    24901 ccctgcggaa ctggaagcgg caattccggc agtgccatcg ccaccgccaa tcaatttatc
    24961 ggcgataatc aacggaatac cggtcacgat aattactgcc acacccaaca ggatccccag
    25021 caaaccagtc tgggcaatca cagtcaaatc aatggtattg cccagcgcga aggcaaagaa
    25081 tggaatcagc gtttgcactg ctttgctgaa aaattctcgt aattcagggt caagattccc
    25141 cagggcaaag cccaccagga acggtaatac tgcgccgacg aaaacatgcg gttcaaacga
    25201 ggcaatcccg gcagtgccca gaataatcat cgtcatgagc ggcccggact ccaacgacat
    25261 caacacaaat gccccagctt cttctttcgt accgtactgc tgcatgatgg aagcgtaaag
    25321 tccgccgttg gtcatatcca tcgccgccac cagcgccagc gttgaaagtc cggcaaagaa
    25381 tccaacttca acaccatgtt ccggaataat gcgcgaggca attgccgcaa ccacccacgc
    25441 gacggcaatt ttagttacca ccagcgtacc ggatttacgc agtaccgttc ccgtcgcgct
    25501 taattttatt gacgccccca tgcaaaaaaa ccacaccgcc agaatgggca ccgtaccggt
    25561 aatcatcccg ttggtgaatg atccaaaata tttccccgcc cccggcgaga aggtgtggca
    25621 cagtgcgcca aggaatagcg ggacgagcat catccccccc gggattttct caatcgagcg
    25681 ttttatctgc atttccatca cctgtcggca tatttttaag ggctgatgcg taattcctca
    25741 atggagcatc agtgttgatg gaaacgaaga tagacattca tttaatcaat aaaagtgatc
    25801 aaaaccacat caattgaaac gctgttttat ttttatcgga tcattgtttt tattttgaaa
    25861 ttgatcacaa aaaaaccacc gccgttggcg atggttcatt ttttaagctg atatgcggcc
    25921 tatacgcctc attgcagcag gcggcaaatg attatttttt cgccgcaaaa cgtgccgctg
    25981 cttcgtccca gttcaccacg ttccagaact ctttaatgta gtccggacgg cggttctgga
    26041 atttcaggta gtaagcatgt tcccacacat ccaggcccag aatcgggaag ccggaagcgc
    26101 cagaaatagc ttcacccatc agcggagaat cctggttagc agtagaaacc accgccagtt
    26161 tatcgccttt cagcaccagc catgcccagc cggaaccaaa gcgggaagct gccgcttttt
    26221 caaattctgc tttgaagtta tcaacggagc cgaagtcacg ttcgatagcc gctttcaggt
    26281 caccctgcag ggtggtgcct tttttcagac ctttccagaa caggctgtgg ttagcgtgac
    26341 cgccagcgtt gttgcgcagt acggttttct tgtctgctgg cagctggtcc agtttggtga
    26401 tcagctcttc aaccggcagg ttggcaaatt ctggcaggct ttccagcgcc gcgttggcgt
    26461 tgtttacgta ggtctgatgg tgtttggtgt ggtggatttc catggtctgc ttatcgaagt
    26521 gcggttccag ggcatcgtaa gcatacggca gggatggcag ggtatagctc atattcatct
    26581 ccagtattgt cgggcggccg attgttaatg ccgcgtaagc agttggttca ttatagttaa
    26641 ttaaatgata ttgaaaatga ttatcaatgc cgtacttttc gtaagggtat ggttttgcag
    26701 gaaaatgccc gagatgtgaa gcaaatcacc cacttaatgc cgtgattgcc agtaaatcga
    26761 caacggcggc aacaggcgaa aggttaatcg acagcacgat ttttacactc atctcgtcgg
    26821 agatgtgaag cgacgaaaaa tgatgaggat aagaagatga gtaacgcgat tacgatgggg
    26881 atattttggc atttgatcgg cgcggccagt gcagcctgtt tttacgctcc gttcaaaaaa
    26941 gtaaaaaaat ggtcatggga aaccatgtgg tcagtcggtg ggattgtttc gtggattatt
    27001 ctgccgtgga ccatcagcgc cctgttacta ccggatttct gggcgtatta cagctcgttt
    27061 agtctctcta cgctgctgcc tgtttttctg ttcggcgcta tgtgggggat cggtaatatc
    27121 aactatggcc tgaccatgcg ttatctcggc atgtcgatgg gaattggcat cgccattggc
    27181 attacgttga ttgtcggtac gctgatgacg ccaattatca acggcaattt cgatgtgctg
    27241 attaacaccg aaggcggacg catgacgttg ctcggcgttc tggtggcgct gattggcgta
    27301 gggattgtaa ctcgagccgg gcagttgaaa gagcgcaaga tgggcattaa agccgaagag
    27361 ttcaatctga aaaaagggct ggtgctggcg gtgatgtgcg gcattttctc tgccgggatg
    27421 tcctttgcga tgaacgccgc aaaaccgatg catgaagccg ctgccgcact tggcgtcgat
    27481 ccactgtatg tcgctctgcc aagctatgtt gtcatcatgg gcggcggcgc gatcattaac
    27541 ctcggtttct gttttattcg tctggcaaaa gtgaaggatt tgtcgctaaa agccgacttc
    27601 tcgctggcaa aaccgctgat cattcacaat gtgttactct cggcactggg cgggttgatg
    27661 tggtatctgc aattcttttt ctatgcctgg ggccacgccc gcattccggc gcagtatgac
    27721 tacatcagtt ggatgctgca tatgagtttc tatgtattgt gcggcggtat cgtcgggctg
    27781 gtgctgaaag agtggaacaa tacaggacgc cgtccggtaa cggtgttgag cctcggttgt
    27841 gtggtgatta ttgtcgccgc taacatcgtc ggcatcggca tggcgaatta atctttctgc
    27901 gaattgagat gacgccactg gctgggcgtc atcccggttt cccgggtaaa caccaccgaa
    27961 aaatagttac tatcttcaaa gccacattcg gtcgaaatat cactgattaa caggcggcta
    28021 tgctggagaa gatattgcgc atgacacact ctgacctgtc gcagatattg attgatggtc
    28081 attccagtct gctggcgaaa ttgctgacgc aaaacgcgct cactgcacga tgcctcatca
    28141 caaaatttat ccagcgcaaa gggacttttc aggctagccg ccaaccgggt aatcagctta
    28201 tccagcaacg tttcgctgga tgttggcggc aacgaatcac tggtgtaacg atggcgattc
    28261 agcaacatca ccaactgccc gaacagcaac tcagccattt cgttagcaaa cgacacatgc
    28321 tgactacttt catgctcaag ctgaccgata acctgccgcg cctgcgccat ccccacgcta
    28381 cctaagcgcc agtgtggttg ccctgcgctg gcgctaaatc ccggaatcgc tccctgtcag
    28441 tcaagattca gcttcagccg ctccgggcaa taaataatat tctgcaaaac cagatcgtta
    28501 acggaagcgt aggagtgttt atcgtcagca tgaatataaa agagatcgcc acgggtaatg
    28561 cgataaggac gatcgttgag tacatgcaga ccattaccgc gccagacaat caccagctca
    28621 caaaaatcat gtgtatgttc ggcaaagaca tcttgcggat aacggtcagc cacagcgact
    28681 gcctgctggt cgctggcaaa aaaatcatct ttgagaagtt ttaactgatg cgccaccgtg
    28741 gctacctcgg ccagagaacg aagttgatta ttcgcaatat ggcgtacaaa tacgttgaga
    28801 agattcgcgt tattgcagaa agccatcccg tccctggcga atatcacgcg gtgaccagtt
    28861 aaactctcgg cgaaaaagcg tcgaaaagtg gttactgtcg ctgaatccac agcgataggc
    28921 gatgtcagta acgctggcct cgctgtggcg tagcagatgt cgggctttca tcagtcgcag
    28981 gcggttcagg tatcgctgag gcgtcagtcc cgtttgctgc ttaagctgcc gatgtagcgt
    29041 acgcagtgaa agagaaaatt gatccgccac ggcatcccaa ttcacctcat cggcaaaatg
    29101 gtcctccagc caggccagaa gcaagttgag acgtgatgcg ctgttttcca ggttctcctg
    29161 caaactgctt ttacgcagca agagcagtaa ttgcataaac aagatctcgc gactggcggt
    29221 cgagggtaaa tcattttccc cttcctgctg ttccatctgt gcaaccagct gtcgcacctg
    29281 ctgcaatacg ctgtggttaa cgcgccagtg agacggatac tgcccatcca gctcttgtgg
    29341 cagcaactga ttcagcccgg cgagaaactg aaatcgatcc ggcgagcgat acagcacatt
    29401 ggtcagacac agattatcgg tatgttcata cagatgccga tcatgagcgc gtacgaaaca
    29461 gaccgtgcca ccggtgatgg tatagggctg cccattaaac acatgaatac ccgtgccatg
    29521 ttcgacaatc acaatttcat gaaaatcatg atgatgttca ggaaaatccg cctgcgggag
    29581 ccggggttct atcgccacgg acgcgttacc agacggaaaa aaatccacac tatgtaatac
    29641 ggtcatactg gcctcctgat gtcgtcaaca cggcgaaata gtaatcacga ggtcaggttc
    29701 ttaccttaaa ttttcgacgg aaaaccacgt aaaaaacgtc gatttttcaa gatacagcgt
    29761 gaattttcag gaaatgcggt gagcatcaca tcaccacaat gaagctaatt gtgaacatca
    29821 tcacgttcat ctttccctgg ttgccaatgg cccattttcc tgtcagtaac gagaaggtca
    29881 cgaattcagg cgctttttag actggtcgta atgaaattca gcaggatcac attatgacct
    29941 ttcgcaattg tgtcgccgtc gatctcggcg catccagtgg gcgcgtgatg ctggcgcgtt
    30001 acgagcgtga atgccgcagc ctgacgctgc gcgaaatcca tcgttttaac aatggcctgc
    30061 atagccagaa cggttatgtc acctggaatg tggatagcct ggaaagtgcc attcgccttg
    30121 gattaaacaa ggtgtgcgag gaagggattc gtatcgatag cattgggatt gatacctggg
    30181 gcgtggactt tgtgctgctc gaccaacagg gtcagcgtgt gggcctgccc gttgcttatc
    30241 gcgatagccg ctccaatggc ctaatggcgc aggcacagca acaactcggc aaacgcgata
    30301 tttatcaacg tagcggcatc cagtttctgc ccttcaatac gctttatcaa ctgcgtgcgc
    30361 tgacggagca acaacctgaa cttattccac acattgctca cgctctgctg atgccggatt
    30421 acttcagcta tcgcctgacc ggcaagatga actgggagta caccaacgcc acgaccacgc
    30481 aactggtcaa tatcaatagc gacgactggg acgagtcgct actggcgtgg agcggggcta
    30541 acaaagcctg gtttggtcgc ccgacgcatc cgggtaatgt cattgggcac tggatttgcc
    30601 cgcagggtaa tgagattccg gtggtcgccg ttgccagcca tgataccgcc agcgcggtta
    30661 tcgcctcgcc gttaaacggt tcacgcgctg cttatctctc ttctggcacc tggtcattga
    30721 tgggcttcga aagccagacg ccatttacca atgacacggc gctggcagcc aacataacca
    30781 atgaaggcgg ggcggaaggt cgctatcggg tgctgaaaaa tattatgggc ttatggctgc
    30841 ttcagcgagt gcttcaggag cggcaaatca acgatctccc ggcgcttatc gccgcgacac
    30901 aggcacttcc ggcctgccgc ttcatcatca atcccaatga cgatcgcttt attaatcctg
    30961 aggcgatgtg cagcgaaatt caggctgcgt gtcgggaaat ggcgcaaccg ctcccggaaa
    31021 gtgatgctga actggcgcgc tgcattttcg acagtctggc gctgctgtat gccgatgtgt
    31081 tgcatgagct ggcgcagcta cgcggtgaag atttctcgca actgcatatt gtcggcggcg
    31141 gctgccagaa cacgctgctc aaccagctat gtgccgatgc ctgcggtatt cgggtgatcg
    31201 ccgggcctgt tgaagcctcg acgctcggca atatcggcat ccagttaatg acgctggatg
    31261 aactcaacaa tgtggatgat ttccgtcagg tcgtcagcac caccgcgaat ctgaccacct
    31321 ttacccctaa tcctgacagt gaaattgccc actatgtggc gcagcttcac tcgacacgac
    31381 agacaaagga gctttgcgca tgaccactca actggaacag gcctgggagc tggcgaaaca
    31441 gcgtttcgcg gcggtgggga ttgatgtcga ggaggcgctg cgccaacttg atcgtttacc
    31501 cgtttcaatg cactgctggc agggcgatga tgtttccggt tttgaaaacc cggaaggttc
    31561 gctgaccggt gggattcagg ctattggtaa ttatccgggc aaagcgcgta atgccagtga
    31621 gctacgagcc gatctggaac aggctatgcg gctgattccg gggccgaaac ggcttaattt
    31681 acatgccatc tatctggaat cagacacgcc agtctcgcgc gaccagatca aacctgagca
    31741 cttcaaaaac tgggttgaat gggcgaaagc caatcagctc ggtctggatt ttaacccctc
    31801 ctgtttctcg catccgctaa gcgccgatgg ctttacgctc tcgcatcccg acgacagcat
    31861 tcgccagttc tggattgatc actgcaaagc cagccgtcgc gtttcggcct attttggcga
    31921 gcaactcggc acaccatcgg tgatgaacat ctggatcccg gatggtatga aagatatcac
    31981 cgttgaccgt ctcgccccgc gtcagcgtct gctggcagca ctggatgagg tgatcagcga
    32041 gaagctggat ccggcgcacc atatcgacgc cgttgagagc aaattgtttg gcattggcgc
    32101 agagagctac acggttggct ccaatgagtt ttacatgggg tatgccacca gccgccagac
    32161 tgcgctgtgc ctggacgccg ggcatttcca cccgactgaa gtgatttccg acaagatttc
    32221 cgccgccatg ctgtatgtac cgcagttgct gctgcacgtc agccgtccgg ttcgctggga
    32281 cagcgatcac gtggtgctgc tggatgatga aacccaggcg attgccagtg agattgttcg
    32341 tcacgatctg ttcgaccggg tgcatatcgg cctcgacttc tttgatgcct ctatcaaccg
    32401 cattgccgcg tgggtcattg gtacacgcaa tatgaaaaaa gccctgctgc gtgcgttgct
    32461 ggaacctacc gctgagctgc gcaagctgga agcggcgggc gattacactg cgcgtctggc
    32521 gctactggaa gaacagaaat cgttgccgtg gcaggcggtc tgggaaatgt attgccaacg
    32581 tcacgatacg ccagcaggta gcgaatggct ggagagcgtg cgggcttatg agaaagcgat
    32641 tttgagccag cgtgggtaaa cactgccgga tgcggcgcga gcgccttatc cggcctacag
    32701 gtcgaatgac catgcgtgct gccaccctca ccccggccct ctccctgaaa gggcgaggga
    32761 gaaaaacata gcctcgtgcg gcaacggagc tgaacaccgc tccggacagt cccctcgccc
    32821 ctacggggag agggttaggg tgaggggaac aagccgcacc atgtctaaac aaggcaacct
    32881 aatttacagg aacacagaac atgcaaaaca ttacccagtc ctggtttgtc cagggaatga
    32941 tcaaagccac caccgatgcc tggctgaaag gctgggacga gcgcaatggt ggtaacctga
    33001 cgctgcgcct ggatgacgcc gatatcgcgc cttatcacgg caactttcat gcacaaccgc
    33061 gctatattcc cctcagccaa cccatgcctt tactggcaaa tacgccgttt attgtcaccg
    33121 gctccggcaa attcttccgt aacgtccagc ttgatcctgc agctaactta ggcgtcgtaa
    33181 aagtcgacag cgacggcgcg ggctaccata ttctctgggg gttaaccaac gaagccgttc
    33241 caacttccga acttccggct cacttccttt cccactgcga gcgcattaaa gccaccaacg
    33301 gtaaagatcg ggtgatcatg cactgccacg ccaccaacct gatcgccctc acctatgtac
    33361 ttgaaaacga caccgcggtc tttactcgcc aactgtggga aggcagcacc gagtgtctgg
    33421 tggtattccc ggatggcgtt ggcattctgc cgtggatggt gcccggcacg gacgaaatcg
    33481 gccaggcgac cgcacaagag atgcaaaaac attcgctggt gttgtggccc ttccacggcg
    33541 tcttcggcag cgggtcgacg ttggatgaaa cattcggttt aatcgacacc gcagaaaaat
    33601 cagcacaagt attagtgaag gtttattcga tgggcggcat gaaacagacc atcagccgtg
    33661 aagagttgat agcgctcggc aagcgcttcg gcgttacacc actagctagt gcgctggcgc
    33721 tgtaaggagc aaacatgatc cgcaaagcct ttgtcatgca ggttaacccc gacgcccacg
    33781 aagagtatca gcgtcggcat aaccccatct ggccggaact ggaagcagtg ttgaaatctc
    33841 acggtgcgca taactacgcc atttacctcg acaaagcgcg taatctgctg tttgccacgg
    33901 tagagattga atctgaagaa cggtggaatg cggtagccag cactgatgtt tgccaacgtt
    33961 ggtggaaata tatgaccgat gttatgcccg ctaacccgga taacagcccg gtgagtagcg
    34021 agctgcaaga agtgttttac ctgccgtaat tgcccaatgt gccagagatg cctccggcac
    34081 aatattttaa atttcgattt gaaaaattaa acatattaca tctcccggta agttatattt
    34141 ccctgataca ttgtgagtaa atcacaaaaa taatgaataa cccattaatg attcatgtgg
    34201 tttatttaaa taaccattat gtgcattact ccgcaaatct gacctttcac tctgtctcaa
    34261 attgtcattg tttaccgaca tatcgcctct catcacttaa tcgctccacc attacaattg
    34321 attaaaatat attttatgga gtccgattaa tggcagctct tactgcaagc tgtattgacc
    34381 tgaatattca gggcaatggc gcttattccg ttctgaagca gttggcgaca atagcgttac
    34441 aaaacggttt tatcaccgac tcacaccagt tcctgcaaac cctgctgctg cgcgaaaaaa
    34501 tgcactctac tggatttggt tccggtgtcg ccgtgccgca cggtaaaagc gcctgcgtta
    34561 aacaaccgtt tgtattattc gcccgcaaag cgcaggctat tgactggaaa gccagcgatg
    34621 gcgaagacgt caattgctgg atctgcctcg gcgtgccgca aagcggcgaa gaggatcagg
    34681 tcaaaatcat cggcacactg tgtcgcaaaa ttatccacca ggattttatt catcaactgc
    34741 aacagggcga cgccgaccag gtgcttgcct tgttaaatca aaccctcagc tcataaggaa
    34801 gtggcgatgg agtcatcctt atgtattgtc gcgatcacca actgccccgc cgggatcgct
    34861 cacacctaca tggtggcgga agccctggaa cagaaagccc gttctctcgg ccataccata
    34921 aaagtggaaa ctcaagggtc cagtggcgtt gaaaaccgct tatccagcga agagattgcc
    34981 gctgccgatt acgtcattct cgctaccggg cgtggcctga gcggtgatga tcgcgcgcgg
    35041 tttgccggga agaaagttta tgagattgcc atctcccagg cgttgaaaaa tatcgaccag
    35101 attttcagcg aattaccgac aaactcgcag ctttttgccg cagatagcgg cgtgaagctt
    35161 ggcaaacagg aagtgcaaag cggcagcgtg atgagccacc tgatggcggg cgtctctgcg
    35221 gcgctgccgt ttgtcatcgg cggtggtatc ctggtggcgc ttgccaacat gctggtgcag
    35281 ttcggtttgc cgtataccga tatgtcgaaa ggcgcgccgt cgtttacctg ggtagttgaa
    35341 tccatcggtt accttggctt cacctttatg atccccatca tgggcgctta tattgcctcg
    35401 tcgattgccg ataaaccggc tttcgctccg g
//
LOCUS       DAJVRF010000027        35084 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJVRF010000027 DAJVRF010000000
VERSION     DAJVRF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.027"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            91..1119
                     /gene="murB"
                     /locus_tag="OQN13_001219"
     CDS             91..1119
                     /gene="murB"
                     /locus_tag="OQN13_001219"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCS1614629.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            1116..2081
                     /gene="birA"
                     /locus_tag="OQN13_001220"
     CDS             1116..2081
                     /gene="birA"
                     /locus_tag="OQN13_001220"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCS1614630.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(2110..3060)
                     /gene="coaA"
                     /locus_tag="OQN13_001221"
                     /gene_synonym="panK"
     CDS             complement(2110..3060)
                     /gene="coaA"
                     /locus_tag="OQN13_001221"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCS1614631.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            3422..3497
                     /locus_tag="OQN13_001222"
     tRNA            3422..3497
                     /locus_tag="OQN13_001222"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3455..3457,aa:Thr,seq:tgt)
     gene            3506..3590
                     /locus_tag="OQN13_001223"
     tRNA            3506..3590
                     /locus_tag="OQN13_001223"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3540..3542,aa:Tyr,seq:gta)
     gene            3707..3781
                     /locus_tag="OQN13_001224"
     tRNA            3707..3781
                     /locus_tag="OQN13_001224"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3740..3742,aa:Gly,seq:tcc)
     gene            3788..3863
                     /locus_tag="OQN13_001225"
     tRNA            3788..3863
                     /locus_tag="OQN13_001225"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3821..3823,aa:Thr,seq:ggt)
     gene            3978..5162
                     /gene="tuf"
                     /locus_tag="OQN13_001226"
     CDS             3978..5162
                     /gene="tuf"
                     /locus_tag="OQN13_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1614632.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVG
                     VLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFR
                     TTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKV
                     LS"
     gene            5392..5775
                     /gene="secE"
                     /locus_tag="OQN13_001227"
     CDS             5392..5775
                     /gene="secE"
                     /locus_tag="OQN13_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCS1614633.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            5777..6322
                     /gene="nusG"
                     /locus_tag="OQN13_001228"
     CDS             5777..6322
                     /gene="nusG"
                     /locus_tag="OQN13_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCS1614634.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            6481..6909
                     /gene="rplK"
                     /locus_tag="OQN13_001229"
     CDS             6481..6909
                     /gene="rplK"
                     /locus_tag="OQN13_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCS1614635.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            6913..7617
                     /gene="rplA"
                     /locus_tag="OQN13_001230"
     CDS             6913..7617
                     /gene="rplA"
                     /locus_tag="OQN13_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCS1614636.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            8030..8527
                     /gene="rplJ"
                     /locus_tag="OQN13_001231"
     CDS             8030..8527
                     /gene="rplJ"
                     /locus_tag="OQN13_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCS1614637.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            8594..8959
                     /gene="rplL"
                     /locus_tag="OQN13_001232"
     CDS             8594..8959
                     /gene="rplL"
                     /locus_tag="OQN13_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCS1614638.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            9279..13307
                     /gene="rpoB"
                     /locus_tag="OQN13_001233"
     CDS             9279..13307
                     /gene="rpoB"
                     /locus_tag="OQN13_001233"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCS1614639.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            13384..17607
                     /gene="rpoC"
                     /locus_tag="OQN13_001234"
     CDS             13384..17607
                     /gene="rpoC"
                     /locus_tag="OQN13_001234"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCS1614640.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(17849..18982)
                     /gene="thiH"
                     /locus_tag="OQN13_001235"
     CDS             complement(17849..18982)
                     /gene="thiH"
                     /locus_tag="OQN13_001235"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCS1614641.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(18979..19749)
                     /gene="thiG"
                     /locus_tag="OQN13_001236"
     CDS             complement(18979..19749)
                     /gene="thiG"
                     /locus_tag="OQN13_001236"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCS1614642.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(19751..19951)
                     /gene="thiS"
                     /locus_tag="OQN13_001237"
     CDS             complement(19751..19951)
                     /gene="thiS"
                     /locus_tag="OQN13_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCS1614643.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(19935..20690)
                     /gene="thiF"
                     /locus_tag="OQN13_001238"
     CDS             complement(19935..20690)
                     /gene="thiF"
                     /locus_tag="OQN13_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCS1614644.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(20683..21318)
                     /gene="thiE"
                     /locus_tag="OQN13_001239"
     CDS             complement(20683..21318)
                     /gene="thiE"
                     /locus_tag="OQN13_001239"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCS1614645.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(21318..23213)
                     /gene="thiC"
                     /locus_tag="OQN13_001240"
     CDS             complement(21318..23213)
                     /gene="thiC"
                     /locus_tag="OQN13_001240"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCS1614646.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAVHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(23294..23394)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(23447..23923)
                     /gene="rsd"
                     /locus_tag="OQN13_001241"
     CDS             complement(23447..23923)
                     /gene="rsd"
                     /locus_tag="OQN13_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCS1614647.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            24018..24791
                     /gene="nudC"
                     /locus_tag="OQN13_001242"
     CDS             24018..24791
                     /gene="nudC"
                     /locus_tag="OQN13_001242"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCS1614648.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            24831..25895
                     /gene="hemE"
                     /locus_tag="OQN13_001243"
     CDS             24831..25895
                     /gene="hemE"
                     /locus_tag="OQN13_001243"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCS1614649.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            25905..26576
                     /gene="nfi"
                     /locus_tag="OQN13_001244"
     CDS             25905..26576
                     /gene="nfi"
                     /locus_tag="OQN13_001244"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCS1614650.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGVDVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            26619..27209
                     /locus_tag="OQN13_001245"
     CDS             26619..27209
                     /locus_tag="OQN13_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCS1614651.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            27396..27668
                     /gene="hupA"
                     /locus_tag="OQN13_001246"
     CDS             27396..27668
                     /gene="hupA"
                     /locus_tag="OQN13_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCS1614652.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            27681..28376
                     /locus_tag="OQN13_001247"
     CDS             27681..28376
                     /locus_tag="OQN13_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCS1614653.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(28378..28803)
                     /gene="zraP"
                     /locus_tag="OQN13_001248"
     CDS             complement(28378..28803)
                     /gene="zraP"
                     /locus_tag="OQN13_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCS1614654.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            29041..30417
                     /gene="zraS"
                     /locus_tag="OQN13_001249"
     CDS             29041..30417
                     /gene="zraS"
                     /locus_tag="OQN13_001249"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCS1614655.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            30414..31739
                     /gene="zraR"
                     /locus_tag="OQN13_001250"
     CDS             30414..31739
                     /gene="zraR"
                     /locus_tag="OQN13_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCS1614656.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(31736..33025)
                     /gene="purD"
                     /locus_tag="OQN13_001251"
     CDS             complement(31736..33025)
                     /gene="purD"
                     /locus_tag="OQN13_001251"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCS1614657.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(33037..34626)
                     /gene="purH"
                     /locus_tag="OQN13_001252"
     CDS             complement(33037..34626)
                     /gene="purH"
                     /locus_tag="OQN13_001252"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCS1614658.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         8367 a         8982 c         9561 g         8174 t
ORIGIN      
        1 tttctacaaa ctcttcctgt cgtcatatct acaagccatc cccccacaga tacggtaaac
       61 tagccttgtt tttgcatcag gaaagcagct atgaaccact ccttaaaacc ctggaacaca
      121 tttggcattg atcataatgc tcagcacatt gtatgtgccg aagacgaaca acaattactc
      181 aatgcctggc agcatgcaac cgcagaagga caacccgttc ttattctggg tgaaggaagt
      241 aatgtacttt ttctggaaga ctatcgtggc acggtgatca tcaaccggat caaaggtatc
      301 gaaattcatg atgaacctga tgcgtggtat ttacatgtag gagccggaga aaactggcat
      361 cgcctggtaa aatacacttt gcaggaaggt atgcctggtc tggaaaatct ggcattaatt
      421 cctggttgtg tcggctcatc acctatccag aatattggtg cttatggcgt agaattacag
      481 cgagtttgcg cttatgttga ttgtgttgaa ctggcgacag gcaagcaagt gcgcttaact
      541 gccaaagagt gccgttttgg ctatcgcgac agtattttta aacatgaata ccaggatcgc
      601 ttcgccattg tagccgtagg tctgcgtctg ccaaaagagt ggcaacctgt actaacgtat
      661 ggtgacttaa ctcgtctgga tcctacaaca gtaacgccac agcaagtatt taatgcggtg
      721 tgtcatatgc gcaccaccaa actccctgat ccaaaagtga atggcaatgc cggtagtttc
      781 ttcaaaaacc ctgttgtatc tgccgaaacg gctaaagcat tactggcgca atttccaaca
      841 gcaccaaatt acccccaggc gggtggttca gtaaaactgg cagcaggttg gcttattgat
      901 cagtgccagc taaaagggat gcaaatgggt ggggctgcgg tgcaccgtca acaggcgtta
      961 gttctcatta atgaagacaa tgcaaaaagc gaagatgtgg tgcaactggc acaccatgta
     1021 agacaaaaag tgggtgaaaa atttaatgtc tggcttgagc ctgaagtccg ctttattggt
     1081 gcatcaggtg aagtgagcgc agtggagaca atttcatgaa ggataacacc gtgccactga
     1141 aattgattgc cctgttagcg aacggtgaat ttcactctgg cgagcagttg ggtgaaacgc
     1201 tgggaatgag ccgggcggct attaataaac acattcagac actgcgtgac tggggcgttg
     1261 atgtctttac cgttccgggt aaaggataca gcctgcctga gcccatccag ttacttaatg
     1321 ctgaacagat attgggtcag ctggatggcg gtagtgtagc cgtgctgcca gttattgact
     1381 ccacgaatca gtaccttctt gatcgtatcg gagagcttaa atcgggcgat gcctgtgttg
     1441 cagaatacca gcaggctggc cgtggtcgcc gggggcggaa atggttttcg ccttttggcg
     1501 caaacttata tttgtcgatg ttctggcgtc tggaacaagg cccggcggcg gcgattggtt
     1561 taagtctggt tatcggtatc gtgatggcgg aagtattacg caagctggga gcagataaag
     1621 ttcgtgtcaa atggcctaat gacctctatc tgcaggatcg caagctggca ggcattcttg
     1681 tggagctgac tggcaaaact ggcgatgcgg cgcaaatagt cattggagcc gggatcaaca
     1741 tggcaatgcg ccgtgttgaa gagagtgtcg ttaatcaggg gtggatcacg ctgcaggaag
     1801 cggggatcaa tctcgatcgt aatacgttgg cggccatgct aatacgtgaa ttacgtgctg
     1861 cgttggaact cttcgaacaa gaaggattgg caccttatct gtcgcgctgg gaaaagctgg
     1921 ataattttat taatcgccca gtgaaactta tcattggtga taaagaaata tttggcattt
     1981 cacgcggaat agacaaacag ggggctttat tacttgagca ggatggaata ataaaaccct
     2041 ggatgggcgg tgaaatatcc ctgcgtagtg cagaaaaata agaaagggaa gtattcgctc
     2101 ccctgcaaat tatttgcgta gtctgacctc ttctaccgca tgattagcac ttttcgtcag
     2161 gattaaactg gcgcgctcac gagtaggtag aatattttgc tttaagttca gccagttgat
     2221 ctctttccac aatgtcatgg cagtcttaat cgcttcttct ttagttaatt tcgcgtagtt
     2281 atgaaaatag gaatccgggt cggtaaaagc cccttcgcgg aatttcagaa aacggttgat
     2341 ataccacgtc tgaagtaagt cttccggtgc atcaacatat atcgaaaaat cgacaaaatc
     2401 agaaacaaat acatgatgtg gatcgtgtgg ataatccatc ccgctctgta agacatttaa
     2461 cccttcaaga attaaaatat caggctgaac aacagtttta tctccatccg ggatcacatc
     2521 ataaataaga tgcgagtaaa caggggctgt aacgtttggc acgccggact tgagatcgga
     2581 aacaaacttc accaggcgat gcatatcata cgattccggg aagcctttct tcttcatcag
     2641 accacgttct ttcagaacct gattagggtg aaggaagcca tctgtagtga tcagttcaac
     2701 acgacgatgt tccggccaac ggcttaatag cgcctgcaat acacgggcgg ttgtactttt
     2761 ccccaccgcg acactgccag caatactgat aatgtaagga atgcgttgcc cgttggtacc
     2821 aagaaactgt tccagaactg cctgacggcg cagattcgag cttatataga agttcagcaa
     2881 acgtgacaaa ggtaaataga tctcggcaac ttcttctaac gagagatctt cattaatacc
     2941 tttgagacgg gcgatctcat cttccgataa cgtcataggt acggaatcac gcagagctgc
     3001 ccactggttg cggtcaaact gtaggtaagg cgtcattaac gtttgctctt ttatactcat
     3061 aagcatgttt ctggcggtca tagctctatc ggcaaagcga tatttgccat ggcgcgtcac
     3121 agttctgttg aatgggttaa tgggcaggag ggtaacacca gatggggagg aataataaga
     3181 aaaaatctct gccaggaagc tatcgttgaa aagcaatgtg acagatattc catctgataa
     3241 aaagaattat ggtttagcag gagcgcattg ttgagcacaa tgatgttgaa aaagtgtgct
     3301 aatctgccct ccgttcggct gtttcttcat cgtgtcgcat aaaatgtgac caataaaaca
     3361 aattatgcaa ttttttagtt gcatgaactc gcatgtctcc atagaatgcg cgctacttga
     3421 tgccgactta gctcagtagg tagagcaact gacttgtaat cagtaggtca ccagttcgat
     3481 tccggtagtc ggcaccatca agtccggtgg ggttcccgag cggccaaagg gagcagactg
     3541 taaatctgcc gtcacagact tcgaaggttc gaatccttcc cccaccacca atttcggcca
     3601 cgcgatggcg tagcccgaga cgataagttc gcttaccggc tcgaataaag agagcttctc
     3661 tcgatattca gtgcagaatg aaaatcaggt agccgagttc caagatgcgg gcatcgtata
     3721 atggctatta cctcagcctt ccaagctgat gatgcgggtt cgattcccgc tgcccgctcc
     3781 aagatgtgct gatatagctc agttggtaga gcgcaccctt ggtaagggtg aggtcggcag
     3841 ttcgaatctg cctatcagca ccacttcttt tctcctccct gttttttcct tctgtttatt
     3901 gcattcaaca agtcgggcat gttgcctggt tgatgtggtg atatcaccga tttatccgtg
     3961 tcttagaggg acaatcgatg tctaaagaaa agtttgaacg tacaaaaccg cacgttaacg
     4021 tcggtactat cggccacgtt gaccatggta aaacaacgct gaccgctgca atcactaccg
     4081 tactggctaa aacctacggc ggtgctgctc gcgcattcga ccagatcgat aacgcgccgg
     4141 aagaaaaagc tcgtggtatc accatcaaca cttctcacgt tgaatacgac actccgaccc
     4201 gtcactacgc acacgtagac tgcccggggc acgccgacta tgttaaaaac atgatcaccg
     4261 gtgctgctca gatggacggc gcgatcctgg tagttgctgc gactgacggc ccgatgccgc
     4321 agactcgtga gcacatcctg ctgggtcgtc aggtaggcgt tccgtacatc atcgtgttcc
     4381 tgaacaaatg cgacatggtt gatgacgaag agctgctgga actggttgaa atggaagttc
     4441 gtgaacttct gtctcagtac gacttcccgg gcgacgacac tccgatcgtt cgtggttctg
     4501 ctctgaaagc gctggaaggc gacgcagagt gggaagcgaa aatcctggaa ctggctggct
     4561 tcctggattc ttacattccg gaaccagagc gtgcgattga caagccgttc ctgctgccga
     4621 tcgaagacgt attctccatc tccggtcgtg gtaccgttgt taccggtcgt gtagaacgcg
     4681 gtatcatcaa agttggtgaa gaagttgaaa tcgttggtat caaagagact cagaagtcta
     4741 cctgtactgg cgttgaaatg ttccgcaaac tgctggacga aggccgtgct ggtgagaacg
     4801 taggtgttct gctgcgtggt atcaaacgtg aagaaatcga acgtggtcag gtactggcta
     4861 agccgggcac catcaagccg cacaccaagt tcgaatctga agtgtacatt ctgtccaaag
     4921 atgaaggcgg tcgtcatact ccgttcttca aaggctaccg tccgcagttc tacttccgta
     4981 ctactgacgt gactggtacc atcgaactgc cggaaggcgt agagatggta atgccgggcg
     5041 acaacatcaa aatggttgtt accctgatcc acccgatcgc gatggacgac ggtctgcgtt
     5101 tcgcaatccg tgaaggcggc cgtaccgttg gcgcgggcgt tgtagcaaaa gttctgagct
     5161 aattgccgat aacatttgac gcaatgcgca ctaaaagggc atcatttgat gccctttttg
     5221 cacgctttcg taccagaacc tggctcatca gtgattttct ttgtcataat cattgctgag
     5281 acaggctctg ttgagggcgt ataatccgaa aagctaatac gcgtttcgat ttggtttgcc
     5341 tcgcgatcgc ggggtgaaaa tgtttgtaga aaacttctga caggttggtt tatgagtgcg
     5401 aataccgaag ctcaaggaag cgggcgcggc ctggaagcga tgaagtgggt cgttgtggtg
     5461 gcattgctcc tggtggcgat tgtcggcaac tacctttatc gcgacattat gctgccgctg
     5521 cgtgcgctgg ccgtagtaat tctgattgct gcagcgggtg gtgtcgcgct gttaacgaca
     5581 aaaggtaaag ctaccgttgc ttttgcccgt gaagcgcgta ccgaagtccg taaggtcatt
     5641 tggccgactc gccaggaaac attgcacacc acgctgattg tggctgcggt taccgcagta
     5701 atgtcactga tcctgtgggg actggatggt attctggttc gcctggtatc ctttatcact
     5761 ggcctgaggt tctgagatgt ctgaagctcc taaaaagcgc tggtacgtcg ttcaggcgtt
     5821 ttccggtttt gaaggccgcg tagcaacgtc gctacgtgag catatcaaat tacacaacat
     5881 ggaagatttg tttggtgaag tcatggtacc aaccgaagaa gtggttgaaa tccgtggcgg
     5941 tcagcgtcgc aaaagcgaac gtaaattctt ccctggctac gtcctcgttc agatggtgat
     6001 gaacgacgcg agctggcacc tggtgcgcag cgtaccgcgt gtgatgggct tcatcggcgg
     6061 tacttccgat cgtcctgcgc caatcagcga taaagaagtc gatgcgatta tgaaccgcct
     6121 gcagcaggtt ggtgataagc cgcgtccgaa aacgctgttt gaaccgggtg aaatggtccg
     6181 tgttaatgat ggtccgttcg ctgacttcaa cggtgttgtt gaagaagtgg attacgagaa
     6241 atctcgtctg aaagtgtctg tttctatctt cggtcgtgcg accccggtag agctggactt
     6301 cagccaggtt gaaaaagcct aacccagcga tcaaaaaagc ggcgatttaa tcgttgcaca
     6361 aggcgtgaga ttggaataca atttcgcgcc ttttgttttt atgggccttg cccgtaaaac
     6421 gattttttat atcacgggga gcctctcaga ggcgttatta cccaacttga ggaatttata
     6481 atggctaaga aagtacaagc ctatgtcaag ctgcaggttg cagctggtat ggctaacccg
     6541 agtccgccag taggtccggc tctgggtcag cagggcgtaa acatcatgga attctgcaaa
     6601 gcgttcaacg caaaaactga ttccatcgaa aaaggtctgc cgattccggt agtaatcacc
     6661 gtttacgctg accgttcttt cactttcgtt accaagactc cgccggcagc agttctgctg
     6721 aaaaaagcgg ctggtatcaa gtctggttcc ggtaagccga acaaagacaa agtgggtaaa
     6781 atttcccgcg ctcagctgca ggaaatcgcg cagaccaaag ctgccgacat gactggtgcc
     6841 gacattgaag cgatgactcg ctccatcgaa ggtactgcac gttccatggg cctggtagtg
     6901 gaggactaag aaatggctaa actgaccaag cgcatgcgtg ttatccgcga gaaagttgat
     6961 gcaaccaaac agtacgacat caacgaagct atcgcactgc tgaaagagct ggcgactgct
     7021 aaattcgtag aaagcgtgga cgtagctgtt aacctcggca tcgacgctcg taaatctgac
     7081 cagaacgtac gtggtgcaac tgtactgccg cacggtactg gccgttccgt tcgcgtagcc
     7141 gtatttaccc aaggtgcaaa cgctgaagct gctaaagctg caggcgcaga actggtaggt
     7201 atggaagatc tggctgacca gatcaagaaa ggcgaaatga actttgacgt tgttattgct
     7261 tctccggatg caatgcgcgt tgttggccag ctgggccagg ttctgggtcc gcgcggcctg
     7321 atgccaaacc cgaaagtggg tactgtaacg ccgaacgttg ctgaagcggt taaaaacgct
     7381 aaagctggcc aggttcgtta ccgtaacgac aaaaacggca tcatccacac caccatcggt
     7441 aaagtggact ttgacgctga caaactgaaa gaaaacctgg aagctctgct ggttgcgctg
     7501 aaaaaagcaa aaccgactca ggcgaaaggc gtgtacatca agaaagttag catctccacc
     7561 accatgggtg caggtgttgc agttgaccag gctggcctga gcgcttctgt aaactaatgc
     7621 ctttacgtgg gcggtgattt tgtctacaat cttaccccca catataatgc ttaatgcaga
     7681 cgtatatccg agatattcgg gttgtggcaa ggcggcaact gagtgagtcg ccaggagcat
     7741 agctaactat gtgactggtg cgaatgaagg aagccaacgc cgccacaagt tgaatagcga
     7801 cggatagaaa agatttgttc gttggagcct ggcctatcca ggcctccgtc gaagaccgca
     7861 ggagtttcgc aagaaactta atcccctgcg tagacggtga cagaacgcta agattattct
     7921 tttatattct ggcttgtttc tgctcaccgt aattaagacg ctctctccgc ttggaggagt
     7981 gaagtgagtt ccagagattt tctctggcaa acatccagga gcaaagctaa tggctttaaa
     8041 tcttcaagac aaacaagcga ttgttgctga agtcagcgaa gtagccaaag gcgcgctgtc
     8101 tgcagtagtt gcggattccc gtggcgtaac tgtagataaa atgactgaac tgcgtaaagc
     8161 aggtcgcgaa gctggcgtat acatgcgtgt tgttcgtaac accctgctgc gccgtgctgt
     8221 tgaaggtact ccgttcgagt gcctgaaaga cgcgtttgtt ggtccgaccc tgattgcata
     8281 ctctatggaa cacccgggcg ctgctgctcg tctgttcaaa gagttcgcga aagcgaatgc
     8341 aaaatttgag gtcaaagccg ctgcctttga aggtgagctg atcccggcgt ctcagatcga
     8401 ccgcctggca actctgccga cctacgaaga agcaattgca cgcctgatgg caaccatgaa
     8461 agaagcttcg gctggtaaac tggttcgtac tctggctgct gtacgcgatg cgaaagaagc
     8521 tgcttaatcg cagttatctt tttaacgcat tcgcttacgt ataaacttat tctgatattc
     8581 aggaacaatt taaatgtcta tcactaaaga tcaaatcatt gaagcagttg cagctatgtc
     8641 tgtaatggac gttgtagaac tgatctctgc aatggaagaa aaattcggtg tttccgctgc
     8701 tgctgctgta gctgtagctg ctggcccggt tgaagctgct gaagaaaaaa ctgaattcga
     8761 cgtaattctg aaagctgctg gcgctaacaa agttgctgtt atcaaagcag tacgtggcgc
     8821 aactggcctg ggtctgaaag aagctaaaga cctggtagaa tctgcaccgg ctgctctgaa
     8881 agaaggcgtg agcaaagacg acgcagaagc actgaaaaaa tccctggaag aagctggcgc
     8941 tgaagttgaa gttaaataag ccaacccttc cggttgcagc ctgagaaatc aggctgatgg
     9001 ctggtgactt tttggtcacc agcctttttg cgctgtaagg cgccagtagc gtttcacact
     9061 gtttgactac tgctgtgcct ttcaatgctt gtttctatcg acgacttaat atactgcgac
     9121 aggacgtccg ttctgtgtaa atcgcaatga aatggtttaa gcgtgatagc aacaggcatt
     9181 gcggaaagtg ttccattttc cggtcaacaa aatagtgttg cacaaactgt ccgctcaatg
     9241 gacagatggg tcgacttgtc agcgagctga ggaaccctat ggtttactcc tataccgaga
     9301 aaaaacgtat tcgtaaggat tttggtaaac gtccacaagt tctggatgta ccttatctcc
     9361 tttctatcca gcttgactcg tttcagaaat ttatcgagca agatcctgaa gggcagtatg
     9421 gtctggaagc tgctttccgt tccgtattcc cgattcagag ctacagcggt aattccgagc
     9481 tgcaatacgt cagctaccgc cttggcgaac cggtgtttga cgtccaggaa tgtcaaatcc
     9541 gtggcgtgac ctattccgca ccgctgcgcg ttaaactgcg tctggtgatc tatgagcgcg
     9601 aagcgccgga aggcaccgta aaagacatta aagaacaaga agtctacatg ggcgaaattc
     9661 cgctcatgac agacaacggt acctttgtta tcaacggtac tgagcgtgtt atcgtttccc
     9721 agctgcaccg tagtccgggc gtcttctttg actccgacaa aggtaaaacc cactcttcgg
     9781 gtaaagtgct gtataacgcg cgcatcatcc cttaccgtgg ttcctggctg gacttcgaat
     9841 tcgatccgaa ggacaacctg ttcgtacgta tcgaccgtcg ccgtaaactg cctgcgacca
     9901 tcattctgcg cgccctgaac tacaccacag agcagatcct cgacctgttc tttgaaaaag
     9961 ttatctttga aatccgtgat aacaagctgc agatggagct ggtgccggaa cgcctgcgtg
    10021 gtgaaactgc atctttcgac atcgaagcta acggtaaagt gtacgtagaa aaaggccgcc
    10081 gtatcactgc gcgccacatt cgccagctgg aaaaagacga cgtcaaactg atcgaagtcc
    10141 cggttgagta catcgcaggt aaagtggttg ctaaagacta tattgatgag tctaccggcg
    10201 agctgatctg cgcagcgaac atggagctga gcctggatct gttggctaag ctgagccagt
    10261 ctggtcacaa gcgtattgaa acgctgttca ccaacgacct ggatcacggc ccgtatatct
    10321 ctgaaacctt acgtgtcgac ccaactaacg accgtctgag cgcactggta gaaatctacc
    10381 gcatgatgcg ccctggcgag ccgccgactc gtgaagcagc tgaaagcctg ttcgagaacc
    10441 tgttcttctc cgaagaccgt tatgacctgt ctgcggttgg tcgtatgaag ttcaaccgtt
    10501 ctctgctgcg cgaagaaatc gaaggttccg gtatcctgag caaagacgac atcattgatg
    10561 ttatgaaaaa gctcatcgat atccgtaacg gtaaaggcga agtcgatgat atcgaccacc
    10621 tcggcaaccg tcgtatccgt tctgttggcg aaatggcgga aaaccagttc cgcgttggcc
    10681 tggtacgtgt agagcgtgcg gtgaaagagc gtctgtctct gggcgatctg gataccctga
    10741 tgcctcagga catgatcaac gccaagccga tttccgcagc agtgaaagag ttcttcggtt
    10801 ccagccagct gtctcagttt atggaccaga acaacccgct gtctgagatt acgcacaaac
    10861 gtcgtatctc cgcactcggc ccgggtggtc tgacccgtga acgtgcaggc ttcgaagttc
    10921 gagacgtaca cccgactcat tacggtcgcg tatgtccaat cgaaacccct gaaggtccga
    10981 acatcggtct gatcaactct ttgtccgtgt acgcacagac taacgaatac ggcttccttg
    11041 agactccgta tcgtaaggtg accgacggtg ttgtaactga cgaaattcac tacctttctg
    11101 ctatcgaaga aggcaactac gttatcgccc aggcgaactc caacctggat gaagaaggcc
    11161 acttcgtaga agacctggta acttgccgta gcaaaggcga atccagcttg ttcagccgtg
    11221 accaggttga ctacatggac gtatccaccc agcaggtggt atccgtcggt gcgtccctga
    11281 tcccgttcct ggaacacgat gacgccaacc gtgcattgat gggtgcgaac atgcaacgtc
    11341 aggccgttcc gactctgcgc gctgataagc cgctggttgg tactggtatg gaacgtgctg
    11401 ttgccgttga ctccggtgta actgcggtag ctaaacgtgg tggtgtcgtt cagtacgtgg
    11461 atgcttcccg tatcgttatc aaagttaacg aagacgagat gtatccgggt gaagcaggta
    11521 tcgacatcta caacctgacc aaatacaccc gttctaacca gaacacctgt atcaaccaga
    11581 tgccgtgtgt gtctctgggt gaaccggttg aacgtggcga cgtgctggca gacggtccgt
    11641 ccaccgacct cggtgaactg gcgcttggtc agaacatgcg cgtagcgttc atgccgtgga
    11701 atggttacaa cttcgaagac tccatcctcg tatccgagcg tgttgttcag gaagaccgtt
    11761 tcaccaccat ccacattcag gaactggcgt gtgtgtcccg tgacaccaag ctggggccgg
    11821 aagagatcac cgctgacatc ccgaacgtgg gtgaagctgc gctctccaaa ctggatgaat
    11881 ccggtatcgt ttacattggt gcggaagtga ccggtggcga cattctggtt ggtaaggtaa
    11941 cgccgaaagg tgaaactcag ctgaccccag aagaaaaact gctgcgtgcg atcttcggtg
    12001 agaaagcctc tgacgttaaa gactcttctc tgcgcgtacc aaacggtgta tccggtacgg
    12061 ttatcgacgt tcaggtcttt actcgcgatg gcgtagaaaa agacaaacgt gcgctggaaa
    12121 tcgaagaaat gcagctcaaa caggcgaaga aagacctgtc tgaagaactg cagatcctcg
    12181 aagcgggttt gttcagccgt atccgtgctg tgctggtagc cggtggcgtt gaagctgaga
    12241 agctcgacaa actgccgcgc gatcgctggc tggagctggg cctgaccgac gaagagaaac
    12301 aaaatcagct ggaacagctg gctgagcagt atgacgaact gaaacacgag ttcgagaaga
    12361 aactcgaagc gaaacgccgc aaaatcaccc agggcgacga tctggcaccg ggcgtgctga
    12421 agattgttaa ggtgtatctg gcggttaaac gccgtatcca gcctggtgac aagatggcag
    12481 gtcgtcacgg taacaagggt gtaatttcta agatcaaccc gatcgaagat atgccttacg
    12541 atgaaaacgg tacgccggta gacatcgtac tgaacccgct gggcgtaccg tctcgtatga
    12601 acatcggtca gatcctcgaa acccacctgg gtatggctgc gaaaggtatc ggcgacaaga
    12661 tcaacgccat gctgaaacag cagcaagaag tcgcgaaact gcgcgaattc atccagcgtg
    12721 cgtacgatct gggcgctgac gttcgtcaga aagttgacct gagtaccttc agcgatgaag
    12781 aagttatgcg tctggctgaa aacctgcgca aaggtatgcc aatcgcaacg ccggtgttcg
    12841 acggtgcgaa agaagcagaa attaaagagc tgctgaaact tggcgacctg ccgacttccg
    12901 gtcagatccg cctgtacgat ggtcgcactg gtgaacagtt cgagcgtccg gtaaccgttg
    12961 gttacatgta catgctgaaa ctgaaccacc tggtcgacga caagatgcac gcgcgttcca
    13021 ccggttctta cagcctggtt actcagcagc cgctgggtgg taaggcacag ttcggtggtc
    13081 agcgtttcgg ggagatggaa gtgtgggcgc tggaagcata cggcgcagca tacaccctgc
    13141 aggaaatgct caccgttaag tctgatgacg tgaacggtcg taccaagatg tataaaaaca
    13201 tcgtggacgg caaccatcag atggagccgg gcatgccaga atccttcaac gtattgttga
    13261 aagagattcg ttcgctgggt atcaacatcg aactggaaga cgagtaattc tcgctcaaac
    13321 aggtcactgc tgtcggggta aaacccggca gcggattgtg ctaactccga cgggagcaaa
    13381 tccgtgaaag atttattaaa gtttctgaaa gcgcagacta aaaccgaaga gtttgatgcg
    13441 atcaaaattg ctctggcttc gccagacatg atccgttcat ggtctttcgg tgaagttaaa
    13501 aagccggaaa ccatcaacta ccgtacgttc aaaccagaac gtgacggcct tttctgcgcc
    13561 cgtatctttg ggccggtaaa agattacgag tgcctgtgcg gtaagtacaa gcgcctgaaa
    13621 caccgtggcg tcatctgtga gaagtgcggc gttgaagtga cccagactaa agtacgccgt
    13681 gagcgtatgg gccacatcga actggcttcc ccgactgcgc acatctggtt cctgaaatcg
    13741 ctgccgtccc gtatcggtct gctgctcgat atgccgctgc gcgatatcga acgcgtactg
    13801 tactttgaat cctatgtggt tatcgaaggc ggtatgacca acctggaacg tcagcagatc
    13861 ctgactgaag agcagtatct ggacgcgctg gaagagttcg gtgacgaatt cgacgcgaag
    13921 atgggtgcgg aagcaatcca ggctctgctg aagagcatgg atctggagca agagtgcgaa
    13981 cagctgcgtg aagagctgaa cgaaaccaac tccgaaacca agcgtaaaaa gctgaccaag
    14041 cgtatcaaac tgctggaagc gttcgttcag tctggtaaca aaccagagtg gatgatcctg
    14101 accgttctgc cggtactgcc gccagatctg cgtccactgg ttccgctgga tggtggtcgt
    14161 ttcgcgactt ctgacctgaa cgatctgtat cgtcgcgtca ttaaccgtaa caaccgtctg
    14221 aaacgtctgc tggatctggc tgcgccggac atcatcgtac gtaacgaaaa acgtatgctg
    14281 caggaagcgg tagacgccct gctggataac ggtcgtcgcg gtcgtgcgat caccggttct
    14341 aacaagcgtc ctctgaaatc tttggccgac atgatcaaag gtaaacaggg tcgtttccgt
    14401 cagaacctgc tcggtaagcg tgttgactac tccggtcgtt ctgtaatcac cgtaggtcca
    14461 tacctgcgtc tgcatcagtg cggtctgccg aagaaaatgg cgctggagct gttcaaaccg
    14521 ttcatctacg gcaagctgga actgcgtggt cttgctacca ccattaaagc tgcgaagaaa
    14581 atggttgagc gcgaagaagc tgtcgtttgg gatatcctgg acgaagttat ccgcgaacac
    14641 ccggtactgc tgaaccgtgc accgactctg caccgtctgg gtatccaggc atttgaaccg
    14701 gtactgatcg aaggtaaagc tatccagctg cacccgctgg tttgtgcggc atataacgcc
    14761 gacttcgatg gtgaccagat ggctgttcac gtaccgctga cgctggaagc ccagctggaa
    14821 gcgcgtgcgc tgatgatgtc taccaacaac atcctgtccc cggcgaacgg cgaaccaatc
    14881 atcgttccgt ctcaggacgt tgttctgggt ctgtactata tgacccgtga ctgtgttaac
    14941 gccaaaggcg aaggcatggt gctgactggc ccgaaagaag cagaacgtct gtatcgctct
    15001 ggtctggctt ctctgcatgc gcgcgttaaa gtgcgtatca ccgagtatga aaaagatgca
    15061 aacggtgaat tagtagcgaa aaccagcctg aaagacacga ctgttggccg tgccattctg
    15121 tggatgattg taccgaaagg tctgccttac tccatcgtca accaggcgct gggtaaaaaa
    15181 gcaatctcca aaatgctgaa cacctgctac cgcattctcg gtctgaaacc gaccgttatt
    15241 tttgcggacc agatcatgta caccggcttc gcctatgcag cgcgttctgg tgcatctgtt
    15301 ggtatcgatg acatggtcat cccggagaag aaacacgaaa tcatctccga ggcagaagca
    15361 gaagttgctg aaattcagga gcagttccag tctggtctgg taactgcggg cgaacgctac
    15421 aacaaagtta tcgatatctg ggctgcggcg aacgatcgtg tatccaaagc gatgatggat
    15481 aacctgcaaa ctgaaaccgt gattaaccgt gacggtcagg aagagaagca ggtttccttc
    15541 aacagcatct acatgatggc cgactccggt gcgcgtggtt ctgcggcaca gattcgtcag
    15601 cttgctggta tgcgtggtct gatggcgaag ccggatggct ccatcatcga aacgccaatc
    15661 accgcgaact tccgtgaagg tctgaacgta ctccagtact tcatctccac ccacggtgct
    15721 cgtaaaggtc tggcggatac cgcactgaaa actgcgaact ccggttacct gactcgtcgt
    15781 ctggttgacg tggcgcagga cctggtggtt accgaagacg attgtggtac ccatgaaggt
    15841 atcatgatga ctccggttat cgagggtggt gacgttaaag agccgctgcg cgatcgcgta
    15901 ctgggtcgtg taactgctga agacgttctg aagccgggta ctgctgatat cctcgttccg
    15961 cgcaacacgc tgctgcacga acagtggtgt gacctgctgg aagagaactc tgtcgacgcg
    16021 gtaaaagtac gttctgttgt atcttgtgac accgactttg gtgtatgtgc gcactgctac
    16081 ggtcgtgacc tggcgcgtgg ccacatcatc aacaaaggtg aagcaatcgg tgttatcgcg
    16141 gcacagtcca tcggtgaacc gggtacacag ctgaccatgc gtacgttcca catcggtggt
    16201 gcggcatctc gtgcggctgc tgaatccagc atccaggtga aaaacaaagg tagcatcaag
    16261 ctcagcaacg tgaagtcggt tgtgaactcc agcggtaaac tggttatcac ttcccgtaac
    16321 accgaactga aactgatcga cgaattcggt cgtaccaaag aaagctacaa agtgccttac
    16381 ggtgcggtac tggcgaaagg cgatggcgaa caggttgctg gcggcgaaac cgttgcaaac
    16441 tgggacccgc acaccatgcc ggttatcacc gaagtaagcg gttttgtacg ctttactgac
    16501 atgatcgacg gccagaccat tactcgtcag accgacgaac tgaccggtct gtcttcgctg
    16561 gtggttctgg attccgcaga acgtaccgca ggtggtaaag atctgcgtcc ggcactgaaa
    16621 atcgttgatg ctcagggtaa cgacgttctg atcccaggta ccgatatgcc tgcgcagtac
    16681 ttcctgccgg gtaaagcgat tgttcagctg gaagatggcg tacagatcag ctctggtgac
    16741 accctggcgc gtattccgca ggaatccggc ggtaccaagg acatcaccgg tggtctgccg
    16801 cgcgttgcgg acctgttcga agctcgtcgt ccgaaagagc cggcaatcct ggctgaaatc
    16861 agcggtatcg tttccttcgg taaagaaacc aaaggtaaac gtcgtctggt tatcaccccg
    16921 gtagacggta gcgatccgta cgaagagatg attccgaaat ggcgtcagct caacgtgttc
    16981 gaaggtgaac gtgtagaacg tggtgacgta atttccgacg gtccggaagc gccgcacgac
    17041 attctgcgtc tgcgtggtgt tcatgctgtg actcgttaca tcgttaacga agtacaggac
    17101 gtataccgtc tgcagggcgt taagattaac gataaacaca tcgaagttat cgttcgtcag
    17161 atgctgcgta aagctaccat cgttaacgcg ggcagctccg acttcctgga aggcgaacag
    17221 gttgaatact ctcgcgtcaa gatcgcaaac cgcgaactgg aagcgaacgg caaagtgggt
    17281 gcaacttact cccgcgatct gctgggtatc accaaagcgt ctctggcaac cgagtccttc
    17341 atctccgcgg catcgttcca ggagaccact cgcgtgctga ccgaagcagc cgttgcgggc
    17401 aaacgcgacg aactgcgcgg cctgaaagag aacgttatcg tgggtcgtct gatcccggca
    17461 ggtaccggtt acgcgtacca ccaggatcgt atgcgtcgcc gtgctgcggg tgaagctccg
    17521 gctgcaccgc aggtgactgc agaagacgca tctgccagcc tggcagaact gctgaacgca
    17581 ggtctgggcg gttctgataa cgagtaatcg ttaatccgca aataacgtaa aaacccgctt
    17641 cggcgggttt ttttatgggt gttattcaga gggcaaattc ttcacggaaa atattaagaa
    17701 aggcagggcg aaacgaggaa gaagcttttc gccctggaca acattcctgc tgacggcact
    17761 accataaaaa aacacaacgc tttcaacaac cttgcgtttt tcatcttccg tagcggctcg
    17821 gaataaattt acgttcttac attccgtctc atagtctttg cgaggcgcgt cccagatagc
    17881 tgtcccagtc tttccatacc ggctgcaaac cctgagcggt taacgcggca gcaaccgctt
    17941 ccggtctgcg atcgtcgtgc ggtgagaact gttccaactc ggggtgatta tcggcatagc
    18001 cacctggctg cgttttcgag aaggcgctga cgttattgat cgccagcgga ataacgcgat
    18061 cgcgaaacca cggtgattcc cgcgtggaga gtgacagttc aatctccggt gcaagcagtc
    18121 ggaaggcgca gatggtttgc actaactggc gttcatccat aatcgacgca ggctcaatgc
    18181 cgccagtaca cgggcgcagg cgcggaaagg agacagagta acggctttgc cagtaatgct
    18241 gttgcagcca tagcaaatgt tcggcaacca tatagcagtc aacgcgccag ttgtcggaaa
    18301 ggccaattag cgcgccgagg cctatcttat caatccccgc acgccccagc cgatccggcg
    18361 tttccagccg ccagaagaag tcctgttttt tgcctttcaa atgatggcgg gtataagtcg
    18421 cctcgtgata tgtctcctga taaaccatca ccccatccag accaagttgc tttaactcgg
    18481 cgtattccgt ctccgccagc ggttgcactt ccatttgtag tgaagagaac tgttcacgca
    18541 gggcggggag atgacgacga aagtaatcca tccccacttt cgcctgatgt tcaccagtga
    18601 ctaatagcag atgttcaaag cccatctccc gtatagcggc gctttccctg gcaatatccg
    18661 cttcatccag cgttttgcgc ttgatgcgat tactcatgga aaatccgcag tacgtgcagt
    18721 cgttagcgca aagattggaa agataaagcg ggacgtagaa actaactgtg ttgccaaatc
    18781 gctgacgggt cagacgctgc gcccgttggg ccagtggttc cagatagcca ctggcggctg
    18841 gcgataacag cgccatcata tcgtcgcggg tgagttgcga ggcatttagc gcccgttcta
    18901 cgtcagcagc cgttttgccg ttgatacgca ggcggatgtc gtcccagtcc agttgtcgcc
    18961 agcgatcgct gaaggttttc atgccgatgc ctccagaaat ccagtcagcg ggctggtggc
    19021 atgagcaaaa tgactgcggc tgcccggtcc ggactgacgt gccagcaggc ctgcttctac
    19081 cgccagacga aatgccttcg ccatgttgac gggatcgtcc gctacggcaa tcgccgtatt
    19141 cactaacacc gcgtcggccc ccatttccag ggcctgcgcg gcatggctgg gaacgccgat
    19201 gccagcatcg acaaccaccg gaactgtggc ctgctggata ataatctcca gcatggcacg
    19261 ggtttccagt ccctgattcg agccaatcgg cgcgccgagc ggcatcaccg ctgcacagcc
    19321 gacttcttcc agacgtttac acaataccgg atcggccccg cagtaaggca gcacgacaaa
    19381 tccctgttgt accagcattt cggcggcttt cagagtttcg atgggatcgg gcaacagcca
    19441 gcgggcgtca gggtgaatct ctaattttaa ccagtttgtg cctaacgctt cacgtgccag
    19501 atgggcggcg aaaatggctt cttccgctgt tttcgccccg gaagtatttg gcagcagggt
    19561 cacacccgcc gcgataagcg gttcgaggat agcgtcgttg tgctggcgca agtcgacacg
    19621 tttcatcgcc agtgtcacca gctggctgcc ggaagcgcgg atcgcctcca ccatcagttg
    19681 tgaagaagcg aatttccctg tgccggtaaa cagatgtgaa tcaaacgttt tgtccgcaat
    19741 acgtaacatc tcaaccccct gcaataacct gaaaaagcag gatctggtcg ccatcctgca
    19801 cgatatgttg cgtccactgc tcacgcggga cgatttgctg attaatcgcc agagccgcgc
    19861 ccgcttgtcg ttggtcgagt tgctccagta gttcgtgaac agtttgcccg gcggcgcact
    19921 gcatcggttg atcgttaaac aggatctgca ttgcttcctc cgcataccgg gcaaccactg
    19981 gcgcggcgca acgccaggct gcgccactgg ctcgatttac cgtcgaacag tcggagttct
    20041 cccgcaggtg tctctatacc gcttaataac ttaatggctt ccagtgcctg caaagtgccc
    20101 ataaccccga ccaccgggcc aaccacgccc gccgtgcggc agttgcgttc tggctcctgg
    20161 ttatctggcc acaggcagcg gtaacacccc tgctcccagg gcggcgtcag taccatcaac
    20221 tgaccgccaa atccgaccgc gctggcggtg ataagcggcg tgttgagtac cacgcaggcg
    20281 gcattaatct cctggcgagt cgccatattg tcggtacagt cgagcaccac atcggcccgt
    20341 gcaaccgcat cttttaacgc ctcacccgtt aaccgttgtt gtaatgctgt cagttgaata
    20401 tcgggattca actgtgtcag tcgctgatgg ctgacctgcg atttcgggcg atcgatatct
    20461 tcagtggtaa agaggatttg tcgttgcaga ttgcttaaat gcacatcgtc gtcatctgcc
    20521 agtaccagcg tcccgacgcc agcccccgcc aggtatagcg cagcaggtgt acccagcccg
    20581 ccaagaccaa taatcagcac ctggctgtcg agcagttttt gctgcccgtc cagagcgata
    20641 tcgtcgagca gaatttggcg gctataacgc ataaagtcac ggtcattcat cgccaactcc
    20701 tgcaatttcc agcaactgtg ccgttgccaa acgccagtct gcggcttgag taatggcgct
    20761 gacgacggcg atactgccga cacccgttgc tatcaccgca ggcgcgcgcg ccagactgat
    20821 accgccaatc gccacggtgg gataatccgc cagtcgctca acatgccgtg ccagctgttc
    20881 cagcccctgc ggtgcagaag gcatctgttt ggtttgcgtc gggaacacat gtcccagcgc
    20941 gatataagag gggcgtgctg ccagcgcgac gtcgatttcc atatcgtcat gtgtcgaaac
    21001 gcccagccgc aggcctgccg cgcggatggc attgagatcg gtggcttgca aatcttcctg
    21061 ccccaaatgg acgccatacg cctgatgctt gatcgccagc cgccagtaat cgttgataaa
    21121 caatcgcgcg ttatagcggc ggcccagcgc aattgccgcc acgacatcgg cttccacctc
    21181 ttcattgcgc cgatctttga tgcgtagctg gagggtacgt acgcctgcat ccaacagacg
    21241 ttcgatccac tgtacgctgt ccaccaccgg gtacagtcct aaacgaaaag gtacaggagg
    21301 aaaatcaggc tgatacatca cgcttcctcc ttacgcaggt agatttctcc gcctctggcg
    21361 cggaagttct ccgacatatc cgccattccc acttcaatgg tttgcgcggc ggcgtaatca
    21421 cgtacttcct ggctgatttt catcgagcag aatttcggcc cacacatgga gcaaaaatgg
    21481 gcgactttac ctgactcttg cggcagggtt tcatcgtgat aagcgcgggc ggtaaacggg
    21541 tcgagggcca gattaaactg gtcttcccag cgaaattcga agcgggcttt cgacatggcg
    21601 ttatcgcgaa tttgcgcgcc tggatgccct ttcgccaggt cagcggcgtg gacggcaatc
    21661 ttataggtga taagcccctg cttaacatct tctttattag gcagacccag atgctctttt
    21721 ggcgttacgt aacagagcat cgcgcagcca aaccagccaa tcatcgccgc accaatcccc
    21781 gacgtgaagt ggtcatagcc cggcgcaata tcggtagtta gcggccccag agtgtaaaac
    21841 ggcgcttcgt ggcagtgctc taactcctcg gtcatattgc ggcggatcat ctgcatcggc
    21901 acgtggcctg ggccttcaat catcacctgc acgtcatatt cccaggcaat tttggtcagt
    21961 tcgcccagcg tatgcagctc ggcaaactgc gcttcatcgt tggcgtcctg aatagaaccg
    22021 gggcgcagac cgtcgcccag cgacagcgaa acgtcataag cggcacagat ttcacaaatt
    22081 tcgcggaagt gttgatagag gaaattttcc tgatgatggg agaggcacca tttcgccata
    22141 atcgaaccgc cgcgagagac gataccggtc aggcgtttcg cggtcatcgg cacatagcgc
    22201 agcagtacgc ccgcatggat agtgaagtaa tccacacctt gctcggcctg ttccagcagc
    22261 gtgtcgcgga acgcttccca ggtaagatct tcggcgatcc cgttaacctt ctccagcgcc
    22321 tggtagatcg gcactgtacc gatcggcacc gggctgttac gcaaaatcca ctcgcgggtt
    22381 tcgtgaatat agcgaccggt ggagagatcc atcaccgtat ccgctcccca gcgcgtggac
    22441 cataccagct tttccacttc ttcttcgatg gaagaggtga ccgccgagtt gccgatattg
    22501 gcgttaactt ttaccaggaa attgcgacca ataatcatcg gctccgattc cggatgatta
    22561 atgttggccg ggataatcgc acgtccggca gcaacttcat cacggacaaa ttccgcagtg
    22621 atattttccg gcagatgtgc gccaaagctc attcccggat gctggtggcg taaaacctcg
    22681 ctacggatgc gctcgcggcc catattctcg cggatggcga tgaattccat ttccggcgtg
    22741 atgatgccct ggcgggcgta gtgcagttgg gtgacacggc gtcctgtttt ggcgcgtttt
    22801 ggcgttaata agccgctaaa acgtagctcg tcgagaccat catctgccag ccgcgttctg
    22861 gtgtaatcgg aactgcgcac ggtaagttct tcggtatcgc cgcgcgcatc gatccacggc
    22921 tggcgtagtt ttgccagccc ttgctgcacg ttaatggcga tctgcggatc accatacggg
    22981 ccggaggtgt cgtagaccgg aatcgcttcg ttttcttcgt actgcggctg ttctttgcta
    23041 ccgccaatta gcgtcgggct aagctggatc tcacgcatcg gcacgcgcac gccggggtgt
    23101 gtgccagtga tataaatgcg ttttgagttg ggaaaggcgg tgccttccag ggtgtcgata
    23161 aaatgttggg cccgggcgcg ttgttcgcgg cgggtcagtt ttgttgcaga catagctcat
    23221 tccaaaaagt caaggacgtg gcttgtcaga cgacggatga agcaagagac gatcgccgca
    23281 aaggcgatgc gatagcagat taactcttgt tcccttcgca ggtattagcc tgatcaggtt
    23341 ccgcggatcc cgaataaacg gtctcagcca gttaaggcac tccgacaaga aattagcccc
    23401 cacaaacggg gcgttgaatg taaattacgc gttaacagcg cagaactcaa gcaggatgtt
    23461 tgacgcgggc ggcgtcaagc accagcagaa tcagcttatc ttccagcaca aagcgcgctt
    23521 ccagcgcctc gccgatgtca gataaaacct gttgaaactc aaggtaatta tcatgatcga
    23581 tggcggtttc caggctggaa tcgtagtaat ccataatctg ttgggtattg gcttcgagtt
    23641 gcggccaaat cttcgcggcg cgcgcgagtt gcccgttgcc ttccagctta tgaagaatgc
    23701 gctcataaat actgaaatgt ccggcagaca agtaatcgac caggctctga caaaaatcat
    23761 caagggcttt ttcgtttagc ctcatgtacg attctttgcc aggcttaatg ccaaccagat
    23821 tgtagtaagc cacgagcaga tgcttacgta catgtagcca gcgatcaacc agtttgttac
    23881 ttcctctgac gcgctccgtc aggttatcga gctggttaag catgattgac tccgcaagtt
    23941 tgtattcaaa aactgctcag tgagaaatgt aaaaaccatg ttaaacatgc cagtgatgca
    24001 aaggtagtgc aagagctatg gatcgtataa ttgaaaaatt agatcacggc tggtgggtcg
    24061 tcagccatga acaaaaatta tggttgccga agggagaatt gccatatggc gaagcggcaa
    24121 atttcgatct tgtgggtcag cgcgcactac aaatcggcga atggcagggg gaacctgttt
    24181 ggttagtaca acagcagcgg cgtcacgata tggggtcggt acgtcaggtc attgatctcg
    24241 atgttgggct gtttcaactg gccggacgag gcgtacaact ggcggagttt taccgatcgc
    24301 ataaatactg tggttactgc gggcatgaaa tgtatccgag caaaaccgaa tgggcgatgc
    24361 tgtgcagcca ttgccgtgag cgttactacc cgcaaatcgc cccctgcatt attgttgcca
    24421 tccgtcgcga tgattcgatc ctcctcgccc agcatacccg ccatcgtaac ggtgtccata
    24481 cagtacttgc cggattcgtc gaagtgggcg aaaccctcga gcaggcagtc gcgcgggaag
    24541 tgatggaaga gagcggaatt aaagttaaaa acttgcgtta cgtgacttct cagccgtggc
    24601 cgtttcctca gtctttaatg accgcgttta tggcggaata tgacagcggc gacatcgtga
    24661 tcgacccgaa agaattgctc gaggcgaact ggtatcgcta tgacgatttg ccgttactcc
    24721 cgccgcccgg caccgtagcg cgccgtctga tagaagatac tgtggcgatg tgtcgggcag
    24781 agtatgagtg atgatacact gaccgcctga cgcactaagg aacagccaaa atgaccgaac
    24841 ttaaaaacga tcgttatctg cgggcgctgc tgcgccagcc cgttgatgtc actccagtat
    24901 ggatgatgcg ccaggcgggt cgctatctac cggaatataa agccacgcgc gcccaggcgg
    24961 gcgattttat gtcgctgtgc aaaaacgccg agctggcgtg cgaagtgact ttgcaaccgc
    25021 tgcgtcgcta cccgctggat gcggcgatcc tcttttccga tatcctcacc gtgccggacg
    25081 cgatggggtt agggctctat tttgaagccg gagaaggtcc gcgttttacc tcgccagtca
    25141 cctgcaaagc cgacgtcgat aaactgccaa ttccggaccc ggaagatgag ctgggttacg
    25201 tgatgaacgc ggtgcgtacc attcgtcgcg aactgaaagg cgaagtgccg ctgattggtt
    25261 tttccggcag cccgtggacg ctggcgacct acatggtgga aggcggcagc agcaaagcgt
    25321 tcaccgtgat caaaaaaatg atgtatgccg atccgcaggc gctgcacgct ctactcgata
    25381 aactggcgaa aagcgtcact ttgtatctga atgcgcagat taaagccggt gctcaggcag
    25441 tgatgatttt cgacacctgg ggcggtgtgc ttaccgggcg cgattatcaa cagttctcgc
    25501 tctattacat gcataaaatt gttgatggtt tactgcgtga aaacgacggt cgccgcgtgc
    25561 cagtcacgct gtttaccaaa ggcggcggac agtggctgga agcgatggca gaaaccggtt
    25621 gcgatgcgtt gggcctcgac tggacaacgg atatcgccga tgcgcgccgc cgtgtgggca
    25681 ataaagtcgc gttgcagggt aatatggatc cgtcgatgct gtacgcgccg cctgcccgca
    25741 ttgaagaaga agtagcgact atacttgcag gtttcggtca cggcgaaggt catgtcttta
    25801 accttggtca cggcattcat caggatgtgc cgccagaaca tgctggcgtg ttcgtggagg
    25861 cagtgcatcg actgtctgaa caatatcacc gctaaggagt gattatggat ctcgcgtcat
    25921 tacgcgctca acaaatcgaa ctggcttctt ctgtgatccg cgaggatcga ctcgataaag
    25981 atccaccgga tctgatcgcc ggagtcgatg tcgggtttga gcagggcgga gaagtgacgc
    26041 gagcggcgat ggtgctgctg aaatatccct cgcttgagct ggtggagtat aaagttgccc
    26101 gcatcgccac caccatgcct tacattccag gttttctttc cttccgcgaa tatcctgcgc
    26161 tgctggcagc gtgggagatg ctgtcgcaaa agccggattt agtgtttgtc gatggtcatg
    26221 ggatctcgca tcctcgccgt cttggcgtcg ctagccattt tggtttgatg gtggatgtgc
    26281 caacgatcgg cgtggcgaaa aaacggctct gcggtaaatt cgaaccgctc tccagcgaac
    26341 cgggcgcgct ggccccactg atggataaag gcgagcagct ggcctgggtc tggcgcagca
    26401 aagcgcgctg taacccgttg tttatcgcta ccggccatcg ggtcagcgtg gacagcgcgc
    26461 tggcgtgggt acaacgctgc atgaaaggct atcgtctgcc ggagccaacg cgctgggcgg
    26521 acgcggtggc ctcggaacgt ccggcgttcg tgcgctatac agcaaatcag ccctaattca
    26581 ggtaaactgc ggataatttc cgtatttgag aactcaacat gttacaaaac ccaattcatc
    26641 tgcgtctgga gcgcctggaa agctggcagc atgtcacttt catggcttgc ttatgcgaac
    26701 gcatgtaccc caattacgcc atgttctgcc agcaaaccgg ttttggtgat gggcaaattt
    26761 accgtcgtat tctcgatctc atctgggaaa cgctgaccgt taaagacgca aaagtaaatt
    26821 tcgacagcca actggagaaa tttgaagaag cgattccttc agccgacgat ttcgatctgt
    26881 acggcgttta tccggcaatc gatgcctgcg tggcgttaag tgaactggtc cattcgcgtt
    26941 tgagtggtga aacgctcgaa cacgcggtgg aagtgagtaa gacctccatc acgaccgttg
    27001 cgatgctgga aatgactcag gctggtcgcg aaatgagcga tgaagagctt aaagaaaacc
    27061 cagctgtaga gcaagaatgg gacattcagt gggaaatatt ccgactttta gctgagtgcg
    27121 aagaacgcga tatcgagctg ataaaaggcc ttagggcaga cctgcgtgag gcgggtgaga
    27181 gcaatattgg tataattttt cagcaataag accagaaaac gtgatttaac gcctgatttg
    27241 tcgtacctgg agtcttccct ttcgcccccc gtctggtcta catttggggg gcgaaaaaaa
    27301 gtggctatcg gtgcgtgtat gcaggagagt gctattctgg catttccgtc gcactcgatg
    27361 cttagcaagc gataaacaca ttgtaaggat aacttatgaa caagactcaa ctgattgatg
    27421 taattgcaga gaaagcagaa ctgtccaaaa ctcaggctaa agctgctctg gagtccactc
    27481 tggctgcaat tactgagtct ctgaaagaag gcgatgctgt acaactggtt ggtttcggta
    27541 ccttcaaagt gaaccaccgc gctgagcgta ctggccgcaa cccgcagacc ggtaaagaaa
    27601 tcaaaattgc cgcagctaac gtaccggcat ttgtttctgg caaggcactg aaagacgcag
    27661 ttaagtaaga ttgcgtggca gtgaacagtt ttaacgaagg ggtggtttca ccccttttgt
    27721 ctttctggcg tcgatcattg atgctggctg gcgctctgct tctcactgcc tgtagtcata
    27781 actcttcact tcctcccttt accgccagtg gatttgctga agaccagggc gcggtacgca
    27841 tctggcgaaa agacagcggc gataatgtgc atctgcttgc cgtgtttagc ccgtggcgca
    27901 gtggcgatac cacgacgcga gagtatcgct ggcagggcga taacctcacg ctcatcaata
    27961 tcaatgttta cagcaaaccg ccggtgaata ttcgcgcgcg ttttgacgat cgcggtgatc
    28021 tgagctttat gcaacgtgaa tccgatgggg aaaagcagca gctttctaac gaccaaatcg
    28081 atttataccg ttatcgtgct gatcagatcc gccagattag cgatgcctta cgtcagggga
    28141 gagtcgtgct gcgccagggg cgctggcatg cgatggaaca gaccgtgacc acctgcgaag
    28201 ggcaaaccat taaacctgat ttagattcgc aggcgatagc gcatatcgag cgccgccaga
    28261 gccgctcttc tgttgatgtc agcgtggcat ggctggaagc gcccgaaggt tcgcaattac
    28321 tgttagtggc aaactctgat ttctgtcgct ggcaacccaa cgagaaaacg ttctgattta
    28381 ccagtggccc atacccatat gaccgccacc accgcagccg ccgtagccca tgcccattcc
    28441 ggctccgcgc ggaatacccg cttcagccat cgcgatatct cgtttcaccc gtaactcatc
    28501 taacgactga cgcaaattct ccatctcttt ggcgaccgcg ttaattttgc tgctatccgg
    28561 tgggttcgcg gctaacaggg cattgtattc ataacgcttc gtcaccagtt gctgttgcag
    28621 tgcgctgctt tgagcgtaaa agtcattatg gattttctgc cacgctgtct gttgttcgct
    28681 ggtcaaaggc gcggcatttt gctgccacat accgtgtccg ccgtgagcaa atgcagatgt
    28741 cgatcccatc gccattgctg aaagcgccat cattaccagg gcaattttcg tgttccgttt
    28801 catggttaat cctccagtgg ttgtcttact tcgggtattg catcttccgt gccaacgatg
    28861 aaacgctgat atgacgggta atctggcatg ataaacgagt aaaaatgact cgcctgctgc
    28921 gggtagcgag tcatttttac tcattgaaac ttaagccttt gtgttacagc gcagggtaag
    28981 cgctgataaa agatggcatg atttctgctg tcagaaaggg atgagcaggc aaagaagaag
    29041 atgcgtttta tgcaacgttc taaagactcc ttagctaaat ggttaagcgc gatcctcccc
    29101 gtggtcattg ttgggctggt agggttgttt gcggtgacgg tgattcgcga ttatgggcgc
    29161 gagactgccg ccgccagaca aacgctgctg gaaaaaggca gtgtacttat tcgcgctctt
    29221 gaatccggct cgcgcgtcgg catggggatg cgcatgcatc atgcgcagca gcaggcctta
    29281 ctggaagaaa tggccgggca gcctggagta cgttggtttg cggtcacgga tgaacaagga
    29341 acaatcgtga tgcatagcaa ctccggcatg gtgggaaaac agctttattc cccgcaggaa
    29401 atgcagcagt tacatccggg agatgaagaa gcgtggcggc ggatcgatag cgcagacggc
    29461 gagcctgttc tggaaattta tcgccagttt caaccgatgt ttgctgctgg aatgcaccgg
    29521 atgcgccata tgcagcagta tgccgcgaca ccacaagcaa ttttcatcgc tttcgacgcc
    29581 agtaatattg tgagtgccga agatcgtgag cagagaaaca ccctgattat cctcttcgcc
    29641 ctggcgacgg tcttgctggc aagcgtattg tcattcttct ggtatcgccg ctatttgcgc
    29701 tcgcgccagc ttctacaaga tgaaatgaag cgcaaagaga agctggtggc gctggggcat
    29761 cttgcggcag gcgttgccca cgaaatccgt aacccacttt cctcgattaa aggactggcg
    29821 aaatactttg ccgagcgcgc gcctgcaggg ggagaagcgc atcaactggc gcaggtgatg
    29881 gcgaaagagg ccgaccgttt aaaccgcgtg gtaagcgagc tgctggaact ggttaagcca
    29941 acgcatctgg ctttgcaggc ggtggatctc aacacgctga ttaaccactc attacagctg
    30001 gtaagccagg atgcgaacag ccgggagatc cagttacgct ttaccgccaa cgacacatta
    30061 ccggaaattc aggccgatcc ggacaggctg actcaggtcc tgttgaatct ctatctcaat
    30121 gctattcagg cgattggtca gcatggcgtg attagcgtga cggccagcga aagcggcgcg
    30181 ggcgtgaaaa tcagcgttac cgacagcggt aagggaattg cggcagatca gcttgatgcc
    30241 atcttcactc cgtacttcac cactaaagcc gaaggcaccg gactggggct ggcggtcgtg
    30301 cataatattg ttgaacaaca cggtggtaca attcaggtcg caagccagga gggaaaaggc
    30361 gcaacgttca ccctctggct tccggtcact attacgcgta agggcccaca aggatgacgc
    30421 acgataatat cgatattctg gtggtggatg atgacattag ccactgcact attttgcagg
    30481 ctttactgcg cggctggggc tataacgtcg cgctggcgaa cagcgggcga caggcgctgg
    30541 agcaggtgcg ggaacaggtt tttgatcttg tgctttgcga tgtgcgaatg gcggagatgg
    30601 acggcattgc cacgctaaaa gagatcaaag cgttaaatcc ggcgattccg gtgctgatta
    30661 tgaccgcata ctccagcgtc gagacggcgg tagaagcgtt gaaaaccggg gcgctggatt
    30721 atctcatcaa gccgctggat ttcgataacc tgcaggcgac gctggaaaag gcgctcgcgc
    30781 atacgcacag tattgacgct gaaacgcctg cggtgtctgc cagccagttc ggtatggtcg
    30841 gtaaaagccc ggcgatgcaa cacctgctca gtgaaatcgc cctcgtcgcg ccatcggaag
    30901 ccacggtgct gatccacggc gattccggca ccggtaaaga gctggtcgcc agggcgattc
    30961 acgccagtag cgcacgtagc gaaaaaccgc tggtaacgct caactgtgcg gcactcaacg
    31021 aatccttgct ggaatctgaa ttgttcggtc acgaaaaagg ggcgttcact ggagccgata
    31081 gacgccggga agggcgcttt gttgaggcgg acggcggcac gctgtttctc gatgaaattg
    31141 gcgatatctc gccgatgatg caggtgcgtc tgctgcgtgc gattcaggag cgcgaagttc
    31201 agcgtgtcgg tagcaaccag actatctcgg ttgatgtccg gttgattgcg gcgacccatc
    31261 gcgatcttgc cgcagaggtg aatgccgggc gttttcgcca ggatctctac tatcgcctga
    31321 atgtggtggc gattgaagta ccatcgctgc ggcaacggcg ggaagatatc cctctgctgg
    31381 ctgtccattt tcttcagcgc tttgccgagc gtaatcgcaa ggcggtaaaa ggctttacgc
    31441 cacaggcgat ggatttgctg atccactacg actggccggg aaatattcgt gaactggaaa
    31501 acgcggtgga acgggcggtg gtgctgctga ccggggaata tatttccgaa cgcgagctgc
    31561 cgctggcgat tgccggtacg ccgatcccac tggggcaaag tcaggatatt cagccgctgg
    31621 tggaagtcga aaaagaagtg attctggcgg cactggagaa aacgggcggc aacaaaaccg
    31681 aagccgcccg tcagttaggg atcacgcgca aaacgctatt ggcaaaactg tcgcgttagt
    31741 tctgctcgcg ttcgatagcg cgccagccga tatctttccg gcagaagcag tcgtcccagt
    31801 gaatatcggt cattaaggca taagcgcgtt tctgcgcttc tgccacggta tgacccagcg
    31861 cggtgacgca cagtacgcgc ccgccgctgg tcactacctg ctcgttatcc gccagtttcg
    31921 tgcccgcgtg gaacactttg ccgcctgcca cttcttccag cggcaggccg tggatcacat
    31981 cgccggtgcg gtaatcgccc ggatatccac ccgcagccat caccacgccg agagaagcac
    32041 gctcatccca ctctgacgtt ttctcgtcca gtttgccttc acaggccgcc aggcagagct
    32101 caaccagatc ggacttcatg cgcagcataa tcggctgggt ttctggatcg ccaaagcggc
    32161 agttaaattc gataaccttc ggattgccct gtttgtcgat catcaggccc gcgtagagaa
    32221 aaccggtgta ggtgttgcct tccgccgcca tgcctttcac ggttggccag atgatacgtt
    32281 ccatggtgcg ctgatgaact tcatcggtta ctaccggcgc gggggagtaa gcgcccatcc
    32341 cgcccgtgtt tggtccggta tctttatcgc ctacgcgttt gtgatcctgg ctggtggcca
    32401 tcggcagcac atgctcgccg tctaccatca cgataaagct cgcttcttcg ccgtcgagga
    32461 actcttcgat aacgatgcga tgacccgcgt cgccaaaagc gttgcccgcc agcatatcgt
    32521 gaacagccgc ttcggcttct tccagcgtca tcgccacgat aacgcctttc ccggctgcca
    32581 gaccgtccgc tttaatgacg atcggcgcac ctttctcacg cagatacgcc agcgcaggtt
    32641 ctacctcggt gaagttctgg tattccgctg tagggatctt atggcgggcc aggaaatctt
    32701 tggtaaacgc tttcgaacct tccagttgcg cggcacctgc ggttgggccg aagattttca
    32761 tcccggcggc gcggaaggta tcgaccacgc ctttcaccag cggcgcttcc gggccgacga
    32821 tggtcagatc aatcttttcg ttttgtgcga aatccaacag cgccgggata tcggtcacgc
    32881 cgatagcaac gttttgcagc gcgggttcca gcgcagtgcc tgcattaccc ggagcaacaa
    32941 aaacagtttc aaccagcggc gactgcgccg ctttccaggc cagcgcgtgc tcgcgcccgc
    33001 cgttaccaat cactaatact ttcatctatt gctccattaa tggcggaagt ggcgcatgtc
    33061 ggtgaagagc atcgcaatac cgtgctcgtc ggcggcggca atcacttcgt catcacggat
    33121 agaaccgcca ggctggatta cgcaggtcac gcctgcagcg gcggcggcat caataccgtc
    33181 gcggaacggg aagaacgcgt cagaagccat cgaggaacct ttcacttcca agccttcatc
    33241 ggcagcttta ataccggcga ttttcgcgga gtacacgcgg ctcatctggc ccgcgccaat
    33301 cccgatggtc atattgtttt tggcatagac gatagcgttg gatttcacga acttcgccac
    33361 tttccagcag aacagcgcat cacgcagttc ctgttcggtc ggctgacgtt gggtgacgac
    33421 gcgcagttct tccgcaccga ccatgcccag gtcacgatcc tgaaccagca gaccgccgtt
    33481 cacgcgtttg aaatcaagac ccggaacacg ctcgccccac tgaccgcagg tcagaacgcg
    33541 tacgttctgc ttggcggcgg tgattttcag ggcttcttcg ctggcggaag gcgcaataat
    33601 cacttcgaca aactgacgag aaatgatggc ctgtgcggtt tccgcatcca gctcgcggtt
    33661 aaaggcaatg atgccgccga atgcggaggt tgggtcggtt ttgtacgcgc gatcgtaagc
    33721 atcaagaatg gaattgccga tagccacgcc gcaagggttg gcgtgcttca caatcacaca
    33781 tgccggctcg gcgaactctt tcacgcactc cagcgccgca tcggtatcgg cgatgttgtt
    33841 ataagagagg gctttaccct gaacctgggt tgcggtagca acggaggctt ctttcacatt
    33901 ctcttctata tagaaggcag cctgctggtg gctgttctcg ccgtaacgca tatcctgctt
    33961 cttaatgaag ttcaggttca gcgtgcgtgg gaagcgaccg gcggcttctt tgctttcacc
    34021 gtggtaagcc ggaaccatgc tgccgaagta gttggcaatc atgctgtcgt aggcggcagt
    34081 gtgttcgaag gctttgatgg cgaggtcgaa acgggttgca agcgtcagcg atccttcgtt
    34141 gtcatccatc tctttaataa tggcgtcgta gtcgctgctc ttcaccacga ttgcgacatc
    34201 tttatggttc ttagcggcgg agcgcaccat ggtcgggccg ccgatatcga tgttctcaac
    34261 cgcatcttcc agcgagcagc cttcacgagc aacagtctgg gcgaacggat acaggttaac
    34321 aaccaccata tcgataggct ggatctgatg ttcttccata atggcatcgt cctggccgcg
    34381 acggcccaga atgccaccat gtactttcgg atgcagggtc ttcacgcgtc catccatcat
    34441 ctccgggaaa ccggtgtaat cggaaacttc ggttaccggc agaccttttt ctgctaacag
    34501 acgggcagtg ccccctgtag acagcagctc cacaccgcgt gcggaaagtg cctgggcgaa
    34561 ttcgacgata ccggctttgt cagaaacact gagcagagcg cggcggactg gacgacgttg
    34621 ttgcatggta aatcccctgg atttgactat tacagagagc gttagctgaa tttttcgcga
    34681 aaaactcagc taacgcccct aacggggcat ccttattttt cgcccgcatt gtaacgaaaa
    34741 cgtttgcgca acgctcgcga atttttctct ttcaatggtg atcacaattt tgacagtggt
    34801 taccgtggga aaaatacaga aattacattg atgattgtgg ataactctgt gcgtaaaaag
    34861 gtataaagcg gggttttgct ggggaatgca gcagtcagtc atttttctgc aatttttcta
    34921 ttgcggcctg cggagaaatc cctataatgc gcctccatcg acacggcgga tgtgaatcac
    34981 ttcacacaaa cagccggttc ggttgaagag aaaaatcctg aaattcaggg ttgactctga
    35041 aagaggaaag cgtaatatac gccacctcgc gacagtgcgc taaa
//
LOCUS       DAJVRF010000028        35004 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJVRF010000028 DAJVRF010000000
VERSION     DAJVRF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35004
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.028"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..835)
                     /locus_tag="OQN13_001253"
     CDS             complement(<1..835)
                     /locus_tag="OQN13_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1614659.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSSPDYH
                     GP"
     gene            927..1013
                     /locus_tag="OQN13_001254"
                     /pseudo
     CDS             927..1013
                     /locus_tag="OQN13_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1417..1893)
                     /locus_tag="OQN13_001255"
     CDS             complement(1417..1893)
                     /locus_tag="OQN13_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCS1614660.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1952..3241)
                     /gene="bioA"
                     /locus_tag="OQN13_001256"
     CDS             complement(1952..3241)
                     /gene="bioA"
                     /locus_tag="OQN13_001256"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCS1614661.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3328..4368
                     /gene="bioB"
                     /locus_tag="OQN13_001257"
     CDS             3328..4368
                     /gene="bioB"
                     /locus_tag="OQN13_001257"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCS1614662.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4365..5519
                     /gene="bioF"
                     /locus_tag="OQN13_001258"
     CDS             4365..5519
                     /gene="bioF"
                     /locus_tag="OQN13_001258"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCS1614663.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5506..6261
                     /gene="bioC"
                     /locus_tag="OQN13_001259"
     CDS             5506..6261
                     /gene="bioC"
                     /locus_tag="OQN13_001259"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCS1614664.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6254..6931
                     /gene="bioD"
                     /locus_tag="OQN13_001260"
     CDS             6254..6931
                     /gene="bioD"
                     /locus_tag="OQN13_001260"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS1614665.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7510..9531
                     /gene="uvrB"
                     /locus_tag="OQN13_001261"
     CDS             7510..9531
                     /gene="uvrB"
                     /locus_tag="OQN13_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCS1614666.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9723..10631)
                     /gene="yvcK"
                     /locus_tag="OQN13_001262"
     CDS             complement(9723..10631)
                     /gene="yvcK"
                     /locus_tag="OQN13_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCS1614667.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10896..11040
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11028..12017
                     /gene="moaA"
                     /locus_tag="OQN13_001263"
     CDS             11028..12017
                     /gene="moaA"
                     /locus_tag="OQN13_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCS1614668.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12039..12551
                     /gene="moaB"
                     /locus_tag="OQN13_001264"
     CDS             12039..12551
                     /gene="moaB"
                     /locus_tag="OQN13_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCS1614669.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12554..13039
                     /gene="moaC"
                     /locus_tag="OQN13_001265"
     CDS             12554..13039
                     /gene="moaC"
                     /locus_tag="OQN13_001265"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCS1614670.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13032..13277
                     /gene="moaD"
                     /locus_tag="OQN13_001266"
     CDS             13032..13277
                     /gene="moaD"
                     /locus_tag="OQN13_001266"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCS1614671.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13279..13731
                     /gene="moaE"
                     /locus_tag="OQN13_001267"
     CDS             13279..13731
                     /gene="moaE"
                     /locus_tag="OQN13_001267"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCS1614672.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13868..14572
                     /locus_tag="OQN13_001268"
     CDS             13868..14572
                     /locus_tag="OQN13_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCS1614673.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14777..15490
                     /locus_tag="OQN13_001269"
                     /pseudo
     CDS             14777..15490
                     /locus_tag="OQN13_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
     gene            complement(15555..16266)
                     /locus_tag="OQN13_001270"
                     /pseudo
     CDS             complement(15555..16266)
                     /locus_tag="OQN13_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(16266..17507)
                     /gene="clsB"
                     /locus_tag="OQN13_001271"
     CDS             complement(16266..17507)
                     /gene="clsB"
                     /locus_tag="OQN13_001271"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCS1614674.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17504..18265)
                     /locus_tag="OQN13_001272"
     CDS             complement(17504..18265)
                     /locus_tag="OQN13_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCS1614675.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18398..18808
                     /locus_tag="OQN13_001273"
     CDS             18398..18808
                     /locus_tag="OQN13_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCS1614676.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18770..19876)
                     /locus_tag="OQN13_001274"
     CDS             complement(18770..19876)
                     /locus_tag="OQN13_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1614677.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19887..21020)
                     /locus_tag="OQN13_001275"
     CDS             complement(19887..21020)
                     /locus_tag="OQN13_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1614678.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21013..22749)
                     /locus_tag="OQN13_001276"
     CDS             complement(21013..22749)
                     /locus_tag="OQN13_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1614679.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKHGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22742..23737)
                     /gene="hlyD"
                     /locus_tag="OQN13_001277"
     CDS             complement(22742..23737)
                     /gene="hlyD"
                     /locus_tag="OQN13_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCS1614680.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23740..24411)
                     /gene="cecR"
                     /locus_tag="OQN13_001278"
     CDS             complement(23740..24411)
                     /gene="cecR"
                     /locus_tag="OQN13_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCS1614681.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24640..26004
                     /gene="rhlE"
                     /locus_tag="OQN13_001279"
     CDS             24640..26004
                     /gene="rhlE"
                     /locus_tag="OQN13_001279"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCS1614682.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26236..26718)
                     /gene="ybiA"
                     /locus_tag="OQN13_001280"
     CDS             complement(26236..26718)
                     /gene="ybiA"
                     /locus_tag="OQN13_001280"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCS1614683.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26838..28988
                     /gene="dinG"
                     /locus_tag="OQN13_001281"
     CDS             26838..28988
                     /gene="dinG"
                     /locus_tag="OQN13_001281"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCS1614684.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIVIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29016..29978
                     /gene="ybiB"
                     /locus_tag="OQN13_001282"
     CDS             29016..29978
                     /gene="ybiB"
                     /locus_tag="OQN13_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCS1614685.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVATGEAATISDGLARVNQAF"
     gene            30119..31204
                     /gene="hcxB"
                     /locus_tag="OQN13_001283"
     CDS             30119..31204
                     /gene="hcxB"
                     /locus_tag="OQN13_001283"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCS1614686.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(31433..31693)
                     /locus_tag="OQN13_001284"
     CDS             complement(31433..31693)
                     /locus_tag="OQN13_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1614687.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31958..32224)
                     /gene="ybiI"
                     /locus_tag="OQN13_001285"
     CDS             complement(31958..32224)
                     /gene="ybiI"
                     /locus_tag="OQN13_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCS1614688.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32298..32975)
                     /gene="ybiX"
                     /locus_tag="OQN13_001286"
     CDS             complement(32298..32975)
                     /gene="ybiX"
                     /locus_tag="OQN13_001286"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCS1614689.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33017..35004)
                     /locus_tag="OQN13_001287"
                     /pseudo
     CDS             complement(33017..35004)
                     /locus_tag="OQN13_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8274 a         8982 c         9500 g         8248 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg
       61 acaatggatt gttataaata ttaacatctg cataaatgtg cagattcatg atactgtctg
      121 gcaatgtgtt cagctgattg ttagggatat atagtgattg cagacgttgt ggaagttcgg
      181 gcagtctgtg tagttggttt tccccaacgt ttaaaagcct cagattttga ggaaggggcg
      241 gtagctcttg taatctgtta tcaaaagcac ttagttcctg tagttcacat ggtaattctg
      301 gtaaggcagt aagctggttg ttgccaacag aaagtttttg tagagccggg ggaagatcag
      361 gtagcgtctc caggctattg agaagggctg agagtgactg caaggaagat ggaagagaag
      421 gcaaacatgt taaaactctg ttaagtgaaa catcaagagc gaccaggtga ggcggaagag
      481 ccgggagcct gctcagtctg ttatcgctgg ctttaagtac agttaaggac ggagggagtt
      541 ctggcagaga gcgtagctca ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt
      601 atgggagaga gcgtaatctg agtaaactta aattgagggc tggctcttga aaagccagac
      661 atattttcag taatcgaact gcctgtgttc ggtcttctgt tgcagcacct tccttggccc
      721 agttatccca gatgcggtga taatccgtga tattttgctc ttctacggat aaacgggata
      781 tacatcctaa accacaaaag gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt
      841 attgatcctc attgtttata tattaatatg ttcgtgcaaa tggagtgagg attctttaaa
      901 aaaacgcgcc tgtaaatagt taagaacagg aagaactgtt ggggatgtgg ctggccgaaa
      961 atgaaggggt gaagttctgg ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc
     1021 cctacctttt catcaagcca gtagtctgcc caccactgca tcatttctct gcgcttatcg
     1081 agatactgag catggttgta aattcagcta acccagtgcg ttgacaaaaa attagcgcaa
     1141 gaggacaaaa aatcaccttg cgctaatgct ctgtatcagg tcactaatac catctaagtg
     1201 gttgattcat agtgactgga tatgttgtgt tttgcagtat tatgcagtct attttttaag
     1261 ctaaatgtat tataatgcat tgatattaat gatttttact gtttctcgtt cagcttttta
     1321 tactaacttg agcgaaacgg gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag
     1381 tgataccaga tggcattgcg ccatctggca gagtgattaa ctaaacatcg cagtaatcga
     1441 ggcgcttgcc agagagtgga aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc
     1501 atcgacatca atacgttcta tatccagcgc gtgaacggta aaaatgtagc gatgagtttc
     1561 gcctttcggc ggtgctgcgc catcgtaccc ggttttacca aagtcggtac gcgtctgcaa
     1621 aacgccgtct ggcattgcta ccagaccaga gccaaaccct tgcggtaata cgcgggtatc
     1681 agcgggtaag ttaacaacta cccagtgcca ccagccggag ccggttggcg catccgggtc
     1741 gtagcaggtg acaacaaaac ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg
     1801 tgaaatatta tcgccatcgt aacccatgcc gttaaagaca tgacgatgcg gcaatttatc
     1861 gccatcgcgc agatcgttac tgatgagttt catgaaccct cctttcttgt ttgcagaaag
     1921 tgtagccaga aaccctcacg cggacttctc gttattggca aaaaaatgtt tcatcctgta
     1981 ccgcgcggtt aaccgctgcg gtcagacgct gcaactgttg cgggagaata atatagggcg
     2041 gcatcaggta aatcagtttg ccaaaaggcc ggatccagac accctgttcg acaaagaatt
     2101 tttgcagcgc cgccatattc accggatgag tggtttcgac cacgccaatg gcccccagta
     2161 cgcgcacatc ggcaaccatt tcggcatcac gggcgggggc aagttgctcg cgcagctgta
     2221 cttcaatatc cgccacctgt tgctgccagt cgccagattc gagaatcgcc aggctggcgt
     2281 ttgctgccgc gcaggccagc ggattgccca taaaagttgg cccatgcata aagcaaccgg
     2341 cttcaccgtt actgatggtt tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca
     2401 ttgtgccgcc ggttaaggct ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt
     2461 cacaggcaaa cagtttcccg gtacgaccaa atccagtggc gatctcgtcg gcaatcagca
     2521 agataccttc gcgatcgcat attttgcgga ttcgttttaa ccattccgga tggtacatgc
     2581 gcatcccgcc tgcgccctgg acaatcggct caatgatcac cgccgcgatt tcatgacgat
     2641 gcgccgccat caggcgggca aagcccacca tatcgcgctc atcccattcg ccatccatac
     2701 ggctttgtgg ggcgggagca aacaagtttt ctggcagata gcctttccac aggctgtgca
     2761 ttgagttgtc cggatcgcac accgacatcg cgccaaaggt atcgccatga taaccattgc
     2821 ggaaggtcag aaaacgctgg cgcgcttcgc ctttggcttg ccagtactgc aacgccattt
     2881 tcatcgccac ttccaccgct acggaaccgg agtccgcgag aaaaacgcac tccagcggtt
     2941 gcggcgtcat cgccaccagt ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc
     3001 caaacatcac atgcgacatg gcatcaattt gcgacttcat cgccgcatta agctgcgggt
     3061 gattgtagcc gtggatcgcc gcccaccagg acgacatacc gtcaaccagg cgtctgccgt
     3121 cagacaaaat cagctcgcaa ccttcggcgc tcaccaccgg ataaaccggc agcggggagg
     3181 tcatggatgt gtatgggtgc cagatatggc gttggtcaaa ggcaagatcg tccgttgtca
     3241 taatcgactt gtaaaccaaa ttgaaaagat ttaggtttac aagtctacac cgaattaaca
     3301 acaaaaaaca cgttttggag aagccccatg gctcaccgcc cacgctggac cttgtcgcaa
     3361 gtcacagaat tatttgaaaa accgttgctg gatctgctgt ttgaagcgca gcaggtccat
     3421 cgccagcatt tcgatcctcg tcaggtccag gtcagcacgt tgctgtcgat taagaccgga
     3481 gcttgtccgg aagattgcaa atactgcccg caaagctcac gctacaaaac cgggctggaa
     3541 gccgagcggt tgatggaagt tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca
     3601 ggatcgacgc gcttctgtat gggcgcggcg tggaagaatc cccacgaacg cgatatgccg
     3661 tacctggaac aaatggtgca gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg
     3721 ggcacgttga gtgaatctca ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac
     3781 cacaacctgg acacctcgcc ggagttttac ggcaatatca tcaccacccg cacttatcag
     3841 gaacgcctcg atacgctgga aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc
     3901 attgttggct taggcgaaac ggtaaaagat cgcgccggat tattgctgca actggcaaac
     3961 ctgccgacgc cgccggaaag cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca
     4021 ctggcggata acgatgatgt cgatgccttt gattttattc gcactattgc ggtagcgcgg
     4081 atcatgatgc cgacctctta cgtgcgcctt tctgccggac gcgagcagat gaatgaacag
     4141 actcaggcga tgtgctttat ggctggcgca aactcgattt tctacggttg caaactgctg
     4201 accacgccga atccggaaga agataaagac ctgcaactgt tccgcaaact ggggctaaat
     4261 ccgcagcaaa ctgccgtgct ggcaggggat aacgaacaac agcaacgtct tgaacaggcg
     4321 ctgatgaccc cggacaccga cgaatattac aacgcggcag cattatgagc tggcaggaga
     4381 aaatcaacgc ggcgctcgat gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg
     4441 tggcgcaagg agccggacgc tggctggtgg cggatgatcg ccagtatctg aacttttcca
     4501 gtaacgatta tttaggttta agccatcatc cgcaaattat ccgcgcctgg cagcagggcg
     4561 cagaaaagtt tggtgtaggt agcggcggtt ccgggcatgt cagcggttat agcgtggtgc
     4621 atcaggcgct ggaagaagaa ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta
     4681 tctctggttt tgccgcgaat caggcagtca ttgccgcgat gatggcgaaa gaggaccgta
     4741 ttgttgccga ccggcttagc catgcctcct tgctggaggc tgccagttta agcccgtcgc
     4801 agcttcgccg ttttactcat aacgatgtcg ctcatctggc gcgactgctt gcttccccct
     4861 gtccggggca gcaactggtg gtgacagaag gcgtgttcag catggacggc gatagcgcgc
     4921 cactggcgga aatccagcag gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg
     4981 cccacggcac gggcgttatc ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa
     5041 aaccagaatt gctggtggtg acttttggca aaggatttgg cgtcagtggg gctgcggtgc
     5101 tttgctccag tacggtggcg gattatctgc tgcaattcgc ccgccacctt atctacagca
     5161 ccagtatgcc gcccgctcag gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg
     5221 atgaggggga tgcacggcgc gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag
     5281 tacaggatct gccgtttacg cttgctgatt catgcagcgc catccagcca ttgatcgtcg
     5341 gtgataacag ccgtgcgtta caactggcag aaaaactgcg ccagcaaggc tgctgggtca
     5401 cggcgattcg cccgccaacc gtacccgctg gtactgcgcg actgcgctta acgctaaccg
     5461 cagcgcatga aatgcaggat atcgaccgtc tgctggaggt gctgcatggc aacggttaat
     5521 aaacaagcca ttgcagcggc atttggtcgg gcagccgcgc actatgagca acacgcagag
     5581 ttacagcgcc ggagtgctga cgtcttactg gcaatgctcc cacagcgtaa atacacccac
     5641 gtactggacg cggggtgtgg gccaggctgg atgagccgcc actggcgaga gcgtcacgca
     5701 caggtgacgg cgttagatct ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct
     5761 gcagaccatt atctggcggg agatatcgaa tccctgccgt tagcgactgc gacgttcgat
     5821 cttgcatgga gcaatctcgc ggtgcagtgg tgcggtaatt tatccacggc actccgcgag
     5881 ctgtatcggg tggtgcgccc cggaggcgtg gtggcgttta ccacgctggt gcagggatcg
     5941 ttacccgaac tgcatcaggc gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt
     6001 ttaccgccag atgaaatcga acagtcgctg aacggcgtgc attatcaaca tcatattcag
     6061 cccatcacac tatggtttga tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt
     6121 gctacgcatc ttcatgaagg gcgcgacccg cgaatattaa cgcgttcgca attgcagcga
     6181 ttgcaactgg cctggccgca acaacagggg cgatatcctc tgacgtatca tctttttttg
     6241 ggagtgattg ctcgtgagta aacgttattt tgtcaccgga acggataccg aagtggggaa
     6301 aaccgtcgcc agttgtgcac ttttacaagc cgcgaaggca gtaggttatc ggacggcagg
     6361 ttataaaccg gtcgcctctg gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc
     6421 gctggcgtta cagcacaaca gcagcctgca gctggattac gcaacagtaa atccttacac
     6481 cttcgcagaa cccacttcgc cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc
     6541 attggtaatg agcgccggat tacgcgcgct tgaacaacag gctgactggg tgttagtgga
     6601 aggtgctggc ggctggttta cgccgctttc tgacactttc acttttgcag attgggtaac
     6661 acaggaacaa ctgccggtga tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc
     6721 gatgttgact gcgcaggcaa tacaacacgc cggactgact ctggcgggtt gggtggcgaa
     6781 cgatgttacg cctccgggaa aacgtcacgc tgaatatatg accacgctca cccgcatgat
     6841 tcccgcgccg ctgctgggag agatcccctg gcttgcagaa aatccagaaa atgcggcaac
     6901 cggaaagtac ataaaccttg ccttgttgta gccattctgt atttggttaa attgcgagcg
     6961 agatcgcgtc ttcgattgac tgcaatttaa ccaattaaat tctaaaataa tcacgaaaaa
     7021 aattttactt ctgcctcatg cggcgaatgt gggaattgcc caggcggcgg gggatagggg
     7081 ctggagacag ttatccacta ttcctgtgga taaccatgtg tattagagtt agaaaacacg
     7141 aggcaagcga gagaatacgc ggcttgcacg cgaattggcg ttaaagacgg ctcaaagaaa
     7201 tatcttttat tttttaactg gttagataaa tgcaatggca gtcactgaac aggcatctct
     7261 tgccataaaa ctgtcatcac tcatcttgac aaatgttaaa aaagccgttg ctttggggat
     7321 aacccggtaa ggccggagtt ttatctcgcc acagagtaaa ttttgctcat gattgacagc
     7381 ggagtttacg ctgtatcaga aatattatgg tgatgaactg tttttttatc cagtataatt
     7441 tgttggcata attaagtacg acgagtaaaa ttacatacct gcccgcccaa ctccttcagg
     7501 tagcgactca tgagtaaacc gttcaaactg aattccgctt ttaaaccttc tggcgatcag
     7561 ccagaggcga ttcgacgtct cgaagagggg ctggaagatg gcctggcgca ccagacgtta
     7621 cttggcgtga ctggctcagg gaaaaccttc accattgcca atgtcattgc tgaccttcag
     7681 cgcccaacca tggtacttgc gcccaacaaa acgctggcgg cccagctgta tggcgaaatg
     7741 aaagagttct tcccggaaaa cgcggtggag tatttcgtct cctactacga ctactatcag
     7801 ccggaagcct atgtaccgag ttccgacact ttcattgaga aagatgcctc ggttaacgaa
     7861 catattgagc agatgcgttt atccgccacc aaagcgatgc tggagcggcg tgatgtggtt
     7921 gtagtggcct ctgtttccgc gatttatggt ctgggcgatc ctgatttata tctcaagatg
     7981 atgctccatc tcacggtcgg tatgattatc gatcagcgcg cgattctgcg ccgactggcg
     8041 gagctgcaat acgctcgtaa tgatcaagca ttccagcgtg gtactttccg cgttcgtggc
     8101 gaggtgatag atatcttccc ggcagaatcg gatgacattg cacttcgcgt ggaactgttt
     8161 gacgaggaag tggaacgatt gtcgttattt gacccgctga ccgggcagat tgtttccact
     8221 attccacgtt ttaccatcta cccgaaaacg cactacgtca caccgcgcga gcgcatcgta
     8281 caggcgatgg aggagatcaa agaagaactg gccgccagac gcaaagtgct gttggaaaac
     8341 aacaaactgc tggaagagca gcggctgacc cagcgtaccc agtttgatct ggagatgatg
     8401 aacgagctgg gctactgttc ggggattgaa aactactcgc gcttcctctc cggtcgtgga
     8461 ccgggtgagc caccgccgac gctgtttgat tacctgcctg ccgatgggct gctggtggtc
     8521 gatgaatctc acgtcaccat tccacaaatt ggcggcatgt atcgcggtga ccgggcgcgt
     8581 aaagagacac tggtggagta cggcttccgc ctgccatcag cgctggataa ccgtccgctt
     8641 aagtttgaag agttcgaagc attagcgccg caaaccatct atgtttcggc gacgccgggt
     8701 aattacgagc tggaaaaatc cggcggcgat gtggtggatc aggtggtgcg tccaaccgga
     8761 ttgcttgacc cgattatcga agtgcggccg gtggcgacac aggttgatga tcttctttcg
     8821 gagattcgtc agcgagcggc aattaacgaa cgcgtactgg tcaccacact gaccaagcgg
     8881 atggcggaag atcttaccga atatctcgaa gaacatggcg agcgcgtgcg ttatcttcat
     8941 tcagatatcg acaccgtcga acgtatggag attatccgcg acttgcgtct gggtgagttc
     9001 gacgtgctgg tagggatcaa cttactgcgc gaaggtctgg atatgccgga agtgtcgctg
     9061 gtggcgatcc tcgacgctga caaagaaggc ttcctgcgtt ccgaacgttc gttgatccag
     9121 actattggtc gtgcggcacg taacgttaac ggtaaagcga ttctctacgg cgataagatc
     9181 accccatcaa tggcgaaagc gattggcgaa accgaacgtc gccgtgagaa acagcagaag
     9241 tacaacgagg aacacggaat tacgccgcaa ggcttgaaca agaaagtggt cgatatcctg
     9301 gcgctggggc agaacattgc caaaaccaaa gcgaagggca gaggaaaatc gcgcccgatt
     9361 gttgagccgg ataatgtgcc gatggatatg tcgcctaaag cgttgcaaca gaaaatccat
     9421 gagctggaag ggttaatgat gcaacacgcg cagaatctgg agttcgaaga agcggcacaa
     9481 attcgtgacc agttgcatca gctgcgtgag ctgtttatcg cggcttcgtg acaggataac
     9541 gaagaagaca gatgacaaaa gcaaaattgc ctgatgtgct acgcttatca ggcctacatg
     9601 atctctgcaa tacattgaat tagcgcggtt tgtaggccgg ataaggcgtt cacgccgcat
     9661 ccggcataaa caacgcgcac tttgtcttca ataggaagcc ggaatttttc ttcggatttc
     9721 cgttaaccta aagcctgtaa cgccttttcc agcgcgttat gtaacaactg gcggtcatga
     9781 cgatacggaa tatcgctggc ctccagtacc tcctgaatca caatccgctc tttcaccgcc
     9841 gagacatcca cttttggccc gacgatgacc gcatcaatga cctttttacc aacatactgc
     9901 tccataattg ccagcttgct ttccagcttc aaattagccg caggtaaact caactcacgc
     9961 cccagattgc cgatatagac catcggcgct ggcgtgcggc gtaatgcctg ggcgatttcc
    10021 ttcagcagca gaattggcat caggctggta taaaaactgc caggcccaat aatgatgaga
    10081 tccgcttcat tgatagcgtg aaccgcctca cgcgttgcgg gtacattcgg cgttaataat
    10141 aactcttgaa tcggcgtagt taactggtcg atattgacct cgccgtaaac ttcatgcccc
    10201 tgatcgtcaa tcgccatcag atcgacggga tgctctgaca ttggaatcaa atgcgtatcc
    10261 actttcagca gattacgaat taaattgatg gcttccagag gccgcacgct aaggtgatcc
    10321 agcgccttta acatcaagtt tccgagatta tgaccggaaa gttcgccatt gccgccaaaa
    10381 cggtattcaa acatcgcgga ggcgacgctc ggttccgtta tcagttggtt gaggcagttg
    10441 cgcatatcgc cccaggcaat gccgccttct gaacggcgaa tacgccccgt cgagccacca
    10501 ttatccgtgg tggtgacgat acccgttaaa cgagaaccca aagacgaaag tgatgagaga
    10561 acgcgtccca gtccatgccc tccgccgaga gcaacgacac gatcaagatc agccagcgta
    10621 cgattgcgca tatagtttcc tggagtcaga ttatccgcgc tacagtagcg caaattgcgg
    10681 ggaaacagca attaacctgc caatattgag gatacaaact attttcttct acctctaaag
    10741 gacgatgcac gctatgcctc cctgatgatg tatatcaaag taaaactgcc attttccctt
    10801 attctgtagc gaaatagcac gatcatgacg ctatatacat gattacatag cgaaagtgtg
    10861 gatggtaaaa atctcattta cacgctagta tcggcttaac cactaaacac tctagcctct
    10921 gcacctgggt caactgatac ggtgctttgg ccgtgacaat gctcgtaaag attgccacca
    10981 gggcgaagga agaaatgact tcgcctcccg tatctggaaa ggtgtacatg gcttcacaac
    11041 tgactgatgc atttgcgcgt aagttttact acttgcgcct gtcgattacc gatgtgtgta
    11101 actttcgttg cacctactgc ctgccggatg gctacaaacc gagcggcgtc accaataaag
    11161 gctttcttac cgtcgatgaa attcgccggg ttacgcgcgc cttcgccagt ctgggcaccg
    11221 aaaaagtgcg cctgacagga ggagagccgt ctttacgccg cgactttacc gatatcatcg
    11281 ccgctgtgcg ggaaaacgac gctatccgcc agattgcggt caccaccaat ggttaccgtc
    11341 tggaacgcga tgtggcgaac tggcgcgatg cgggacttac tggcattaac gtcagtgtcg
    11401 acagtctgga cgcccgccag tttcacgcta ttaccgggca ggataaattc aaccaggtca
    11461 tggcagggat cgatgctgca tttgaggtcg gttttgagaa ggtcaaagtc aataccgtgc
    11521 tgatgcgtga tgttaatcat caccagctcg acacctttct gaactggatc cagcatcgcc
    11581 ctatccagct gcgtttcatc gaactgatgg aaacgggcga gggcagtgag ctcttccgta
    11641 agcatcacat ctctggtcag gttctgcgtg acgagctact gcgtcgcggc tggatccacc
    11701 aattacgtca acgcagcgac ggtcctgcgc aagtcttttg ccatccagat tacgccggag
    11761 agattggcct tatcatgccg tatgaaaaag acttctgcgc cacttgcaac cgcctgcgcg
    11821 tttcctccat tggtaaactc catctctgcc tgtttggtga aggcggcgtt aacctgcgcg
    11881 atctgctgga agacgatacc cagcaacagg cgctggaagc gcgtatttca gcggcgctgc
    11941 gggagaagaa acagacccat ttcctgcatc aaaacaacac cggtattacg caaaacttat
    12001 cgtacattgg cggctaaaac gtcaaaagga gagatcagat gagtcaggta agcactgaat
    12061 ttatcccgac ccgtattgct attcttacgg tttctaatcg tcgcggtgaa gaagacgata
    12121 cctccggtca ctatttgcgc gattcggcgc aagaagcggg ccatcacgtt gtcgataaag
    12181 ccattgtgaa agaaaaccgc tacgctattc gcgctcaggt atctgcgtgg atcgccagcg
    12241 acgatgtaca agtggtgttg attacggggg ggactggcct gacggaaggt gatcaggctc
    12301 ccgaagcatt gctgccgttg ttcgaccgtg aagttgaagg ttttggtgaa gtgttccgga
    12361 tgttgtcgtt tgaagagatt ggcacttcca cgttgcaatc tcgtgcggta gcgggcgtcg
    12421 ccaacaaaac gctgattttc gccatgccgg gttcgaccaa agcgtgccgt accgcatggg
    12481 aaaatatcat cgcgccgcag ctggatgccc gtacgcgtcc gtgtaatttc catccacatt
    12541 tgaagaaata agtatgtcgc aactgaccca tatcaacgcc gctggcgaag cgcacatggt
    12601 ggatgtctcc gccaaagcgg aaaccgtgcg tgaagcgcgg gcggaagcct ttgtcaccat
    12661 gcgcagcgag acgctggcga tgattattga tggtcgccac cacaaaggcg acgtatttgc
    12721 cactgcgcgt attgtcggta ttcaggcggc aaaacgcacc tgggatctga tcccgctgtg
    12781 tcacccgctg atgctcagca aagttgaagt caatttacag gccgagccgg agcacaatcg
    12841 ggtgcgtata gaaaccttat gccgcctgac cgggaaaacc ggtgtcgaaa tggaagcgtt
    12901 aaccgcggcc tccgtggcgg cgctgaccat ttatgacatg tgcaaagcgg tgcaaaaaga
    12961 tatggtgatt ggtccggtac gtttgctggc gaagagcggc ggcaagtcgg gtgactttaa
    13021 ggtggaagcg gatgattaaa gttctttttt tcgcccaggt gcgcgagttg gtgggaacag
    13081 atgcaaccga agtggctgcg gattacccaa ccgttgaagc gttacgccag cacctggctg
    13141 cgcagagcga tcgctgggcg ctggcgctgg aagatggcaa attgctggct gccgtcaacc
    13201 agacgctggt gagttttgac catccgctga ctgacggcga cgaagtagct ttcttcccgc
    13261 cggtaaccgg aggttaagat ggcagaaacc aaaattgttg tcggcccgca gccgttcagc
    13321 gtaggagaag agtacccgtg gctggctgag cgtgacgaag acggtgcagt ggtcacgttt
    13381 accggtaagg tgcgcaacca taacctgggt gacagcgtca aagcattaac cctcgaacac
    13441 tatccgggga tgactgaaaa agcactggca gaaatcgttg atgaagcgcg taaccgctgg
    13501 ccgctggggc gcgtcacggt gattcaccgc atcggggaat tatggccggg cgatgaaatc
    13561 gtttttgtcg gtgtcaccag tgcgcatcgc agcagtgcgt ttgaagccgg gcagtttatt
    13621 atggattatc tcaaaacccg cgcaccgttc tggaagcgcg aagccacgcc ggaaggcgac
    13681 cgctgggttg aagcccggga gagcgatcag caggcggcaa aacgctggta gttttttgtt
    13741 agccggataa ggcaccgctg cgtccggcaa tttcaccgct cagtacatgc gtttacgctg
    13801 cgtcgatgca cagcctcatc attttgcagt atccttaaga tattccttat atcttcagga
    13861 gatcgtcatg gacagattcc cacgttctga ttcaatcgta caaccccggg ctggcttgca
    13921 aacttatatg gcgcaagtct atggctggat gaccgttggc ttgttgctga ccgcatttgt
    13981 tgcctggtat gcggctaatt ccgcggccgt gatggagctg ttgttcacta accgtgtctt
    14041 tttaatcggt ctgatcatcg cgcaattagc attggttatt gtgttatcag cgatgattca
    14101 aaagctgagc gcaggtgtaa cgacgatgct ctttatgctt tattcggcgc tgacgggtct
    14161 tacgctttcc agtatattca ttgtctatac cgctgcttct atcgccagta ctttcgtcgt
    14221 tactgccggg atgttcggcg caatgagcct gtacggttac accacgaagc gcgatttaag
    14281 tggctttggc aatatgctgt ttatggcgtt aatcggcatt gtgctggcat cgctggtcaa
    14341 cttctggctg aaaagcgaag cattgatgtg ggcagttacc tacatcggcg tgattgtctt
    14401 tgtcggattg acggcgtatg acactcagaa attgaaaaat atgggtgagc agattgatac
    14461 ccgcgacacg tcgaacctgc gcaaatattc cattcttggc gcgttaacct tgtatctgga
    14521 cttcatcaac ctgttcctga tgttgttacg gatcttcggc aaccgccgtt aattcttttt
    14581 caccatcact atcccggcca tgtgtcggga tttttttctt actaaaaacg acacttaaca
    14641 agtaccaggt ataaaatatg ccttatgagc gagcggaatc gttttccgta tccataaata
    14701 caaaagtatt ttgtatgcgt ggttttaaat ttattgatgg tgaatattaa tattttttct
    14761 aaggacaact aaaagaatgg aatcatactc gcaaaacagc aataaattag attttcagca
    14821 cgaggccagg atataaaacg gtatatggct cattaccgct ttaggtttgg tggcaaccgc
    14881 aggactagcc tggggagcta agtatctcga aattacggca accaaatatg attcaccacc
    14941 aatgtatgtc gctatagggt tattattact ttgtatgtat ggcttaagta aggatatcaa
    15001 caagataaat gccgccatcg cgggcgtaat atatctgttt ttactctctt tggtggcgat
    15061 tgtcgttgca agtttagttc ctgtatatgc cattatcatc gtgttcagca ctgcgggcgc
    15121 gatgttttta atcagtatgc tggccggttt attatttaat gttgagcctg gttctcaccg
    15181 ttttatcatt atgatgacgt tgacagggtt ggccctggta atcatcgtga atgcggcatt
    15241 aatgagtgaa cggcccattt gggtaataag ttgcttaatg attgtgttat ggccaggcat
    15301 tatctcgcat ggacgaaata agctccttga actggcagga aaatgccata gtgaagagtt
    15361 gtggagtccg gttcgttgcg cttttacagg tgcattaaca ctctattact attttatcgg
    15421 cttctttggg atacttgccg cgatagctat aacgcttgtc tggcaaaggc atacgcgttt
    15481 ttttcattag ccgtaaatgc acatactaaa gaacttaact aaacttcaca tcgccgcttc
    15541 atttttcgcc cgtagcccag tacaaagaaa tagttcacgc tttgaccaag taacagccag
    15601 ataatcgccc ccattaccat ccagttaacg ctggaaatca gcatctgagc gagggcgaat
    15661 ttccatgaag gcagcaccag cttttgccct ttgatggtca tatggcggtg cttcgcgaaa
    15721 gcgcaaaacc acaaataaac cgcgataatc atcagtaagc caatgccgag aatacgcagc
    15781 gtagtttgat cgacatacca gtgatccggc aactccacca cgcctgcggt aaagataatc
    15841 cctgccagta aaatatagcc cagccagttg gtggtaatac tgagcgagaa aatccgcgta
    15901 atagtgctac cgggcaaccc cagacgagag tacaaacgat agcgcatacc aatgccgccg
    15961 acccaggtac tgagcgtcag gttgaaggcg tagcagataa acgacaccag catcacctgg
    16021 cgcttcgcca gtttgtgacc gcagtaaaag cgggcgagca ggtcatagca gccgtaaatc
    16081 agatagctga cgaccaccag cgaccgcact aagcagcgca acgcgattgt agtcgcggat
    16141 gaccttccag acctcttccc agtccacttt tttggcgtag accaccagca acacaatcac
    16201 cgcgataaaa aacagccagg tgaggatctt ctttgctaag cgccagcgcg ggtgtgattt
    16261 actcatcagg gttttacccc cgtgttttca gtttctaccc gatcctgcgt ttccattgtc
    16321 ggttgtgcgg gcggatcaac ctgcgccaga cgtggcgtgt gtgccggaag ccagccaacc
    16381 agcgccggga agtggcgtaa aaagtggaac gccagcacgc ttttggtcag gttccaccag
    16441 gtgcgtttgg gcagcatggt ttcatccacc tgctgacaat ctgcggcgat aatgccgttc
    16501 agattatcgc gcagcgtctg gttaaaatta cgatcgtgga tgatgacatt tgcttcgaga
    16561 ttcagtgaca aactgagcgg atcgagatta ctggacccta ctgtcgccca gtgatcgtcc
    16621 atcaatgcta ctttgccgtg gagcgggcgg cggcggtact caaacacctg aacgccgcct
    16681 ttaaccagat agttatacag caagcgcgcg ccgactctga caatcggcat atccggttcg
    16741 ccctgaatga tcagtttgat ccgcaccccg cggcgtgccg ctttacgcaa ggcgtgtaaa
    16801 aatcgatagc cggggaagaa gtaggcgttg gcgataatga cttcccgccg cgcctgagtg
    16861 agcattttca gatagtggcg ttctatatca tcgcggtgtt cttcgttatc gcgccagacc
    16921 agcaatacct gcgcgtctcc cggctggcgg ttctcttccg ctttgtgatg acgtcgccac
    16981 cagcgtcgtg ccgcgctctg tccaggcagg ttttccagct caaactggag aatatcttcg
    17041 acaatcggcc cttcaaggcg taccgcgtaa tcctgtttag cctctggacc gtagctggac
    17101 atatgctcgg cggagtaatt cagcccgcca ataaaggcga tacgcgcgtc gatcaccaca
    17161 attttgcgat gcatccggcg aaacacattg gtgcgcatac caaaaaggcg agggcgggga
    17221 tcgtagtagc ggaacactac gccagctgcc gtcagttcat tgacaaactc atcgctgaga
    17281 tccggcgaac cgtagccatc cagcaagact tccgctttaa ccccgcgttg cgctgctgcc
    17341 agtagtgccg catgcagctg tttgccgacg ttatcctcaa accagataaa cgtttcaaga
    17401 atgatgcgtt cttgtgcctc gccaatcgcc ttaaacaccg cgggataata ttgctcgccg
    17461 ttttccagca actggatctt attgccttcg cgccagctac atttcataaa tgaatctccg
    17521 cacttaaagg ggcatgatca gaaaggtgtc gccatgtccg cagcggcaac gcggttggcg
    17581 cgctggcgct ggcatttttg acgtagatcc tgtccagtcg tagtagagga aattgcaccg
    17641 gaaacgtgcg cgccgggcgt ccgtgggcgc gggtaaaaat ctcatccagt ccggcctgca
    17701 cttttaacgg atgattagct ttttgccgcc agtcattgaa atcacccgcc accaataccg
    17761 gttcgccgtc cggtagctca ttcacccatt cggcgagcat cgcaagctgc gcctgacggt
    17821 gcgcctcacg caggcccaga tgtacgcaca tcacatgaat cgcttttccg gtcatcggcg
    17881 gcacaatgcg gcagtagagc acgccgcgct tttccgcacc atcgaccgaa acatcgcgat
    17941 tctcataatg ttcaatggga taacgcgaca gtacggcgtt gccgtgatgc ccttccgggt
    18001 atacggcatt gcgaccgtag gcaaaatcgc tccacatagt gtcggcgaga aactcgtagt
    18061 gcgaggtatc gggccagttt tccacatgca gcggatgaac ttcgtgcgcg cccatcactt
    18121 cctgcaggca aacaatatcg gcgctgacgg tacgcacggc atcgcgaagt tccggcaaaa
    18181 tgaagcgtcg gttaaacgcg gtaaagcctt tgtgaatgtt gatggtgagc accttgaacg
    18241 aaaattgttg tgtttgatcg ggcataattt tcctgtcttt gcctcttatc tcattgaaat
    18301 agtgtagtcg gcgtcacaaa aaggtgcggt cttacggaat tttccgtaaa gttcggtact
    18361 ctgagtaagt agagataaat tcttcaggag agaagccatg aagtggcaac aacgtgttcg
    18421 tgtcgcaacg ggtctaagtt gctggcagat tatgttgcat ttactggtag tggcgctgct
    18481 ggtggtgggc tggatgagta agactctggt tcacgtcggc gtgggattat gcgcactgta
    18541 ttgtgtcacg gtagtgatga tgctggtgtt tcagcgccac cccgagcaac gctggcgtga
    18601 ggtggcagac gtgctggaag agctgaccac gacctggtat tttggcgcag cgctgattgt
    18661 gctgtggctg ttgtcccgcg tactggaaaa caactttttg ctggcaattg cagggctggc
    18721 gatccttgct ggcccggcgg tagtgtcttt gctggcgaaa gataagaagt tacatcacct
    18781 tacgtctaaa catcgcgtac gccgctgacc ctgtcgtggc cgttatcacc agtagcggcc
    18841 acaaactatt ccacacaata tccagactcg catccttcaa ataaatctgc ttggtaatgt
    18901 ccgtaaagtg gcgaataggg ttaatccacg tcaggttttg cagccatact ggcatgtttt
    18961 ccaccggaga aacgtaaccg gaaaggagaa tggcgggcat cataaaaaca aacacgccga
    19021 taaacgcctg ctgttgtgtc gagcagagtg atgaaatcaa cagaccgaat cccaccagcg
    19081 ataaaccgta aatcaccatc gtaaagtaga acagcgccag cgatccggcg aaggggattt
    19141 gatacgccca gataccaatc gccagcacaa tggtggcctg gaaggtggcg acaattaacg
    19201 ccggtacggc tttgccgatg aagatctgcc aggtggtgag cggcgaaacc agtagctgat
    19261 cgagcgtacc ttgttcacgt tcgcgggcga cggaaagtga agtgacgatc attacgccga
    19321 tagtggtgat catggcgatc agcgacggca ccacaaacca tttgtagtca agattcgggt
    19381 tataccagtt gcgtaccacc agctcgctgt tgttaggttt cggttttcct tccagcagct
    19441 cctgctgata atttttgacg atctgttgca ggtagttggc ggcaatttgc gcactgttgg
    19501 agttacgccc gtcgaggatc aactgcaacg gcgcggtctg gaaggtatcc agtttgcgcg
    19561 agaagtcagc cgggaaacgc accagtagta acgccttttg tgtgtcgatg gttgggcgga
    19621 tctcctgtgg gcttttcagc agcagcacat gggtaaaggc gctggcgcgg gcaaaacgtt
    19681 gggtcagctc caccgaatgc tcgccgttat cttcatcgta gatggcgatg gtggcgttag
    19741 tcacttccag cgtggcggcg aacgggaaca ggatcacctg aataagcaca ggtaaaatca
    19801 gaatcgcgcg ggtttgtggt tcgcgcagca acgactgcaa ctctttgcgg attaacgtcc
    19861 ataagcgatg aaacatgctc ttctccctaa tccagccgac gtttggtttt cagccacgtc
    19921 aggccgataa acatcaccgc cgaagcgatc aaaaacagca cgtttaccac cagcaccact
    19981 ggaatattcc cggcgaggaa caggctttgc agggtgctga cgaaataacg agcgggaata
    20041 atgtacgtca ccgcgcggat caccgcgggc atactgtcga tctgaaaaat aaagccggaa
    20101 agcataatcg acggcaggaa cgcggcgttc agggcgacct gagcggcatt gaactggttg
    20161 cgggtaatcg tggaaatcag cagccccatc cccagggtac tgagtaaaaa caggctggag
    20221 ataaaaaaca gaataagcag cgaaccgcga tacggcacgc cgagaataaa cactgacacc
    20281 agcatacaca gcagcatcgc cagcatcccg agaaagtaat aagggatcag cttacacagc
    20341 agcagttccg tgcgggtaat ctccgtagag agcagagcct ccatggtgcc gcgttcccat
    20401 tctcgcgcca ccaccagcga ggtgagaatc gcgccgatga ccgtcatgat gatggtcact
    20461 gcaccgggga taatgaagtg ctggctaatc gccgccgggt taaaccagta gcgggtttgt
    20521 acatcaataa gcggttcaaa agtctgcccg ttgtcctccg ctcgctgcat ttgccagatc
    20581 tgccagatcc cttcgacata cccctgtaca aagttagcgg tattcggctc actgccgtcg
    20641 gtgatcacct gaatcggtgc ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc
    20701 ggaataacca ccagaccgcg aattttcccc gcctgcattt tggcgatcag ttcctgacgg
    20761 ttatcgctga tggtggcgtc gatgtagggc gaaccggtca tggtgtgggt gaaatccagc
    20821 gccgcttcgc tacgctgttc cagcaagatc ccgacccgca gcttgctgga gtcgaggtta
    20881 atgccataac caaaaataaa cagcagtagc agtgggatca ctaccgcaat cagccagcta
    20941 ctcggatcgc gaacgatctg ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag
    21001 gaaaggatcg ggttactcat tgctatgctc cttatcccag tcgtggatca actgaataaa
    21061 ggcttgctcc atggtgggat cgggttgctc atcgttagcc gactgtgctt tcaaatcgtc
    21121 cggcgtaccg ctggcgatta atttcccacg gtacaccagg ccgatgcggt cgcaatattc
    21181 cgcttcatcc ataaagtggg tggtgaccat caccgtgacg cctttctcta ccatgctgtt
    21241 gatatgtagc caaaattcac ggcgggtgag gggatcaacg ccggacgtcg gttcgtcgag
    21301 aaacagaatg tccggttcat gcatcagcga acaggccagc gccagccgct gtttaaaacc
    21361 taatggcagt tcatcggtgg cgtgggaggc gatacttttc aggccgaacg cctcgctcat
    21421 gcgggagatt ttttcgttct gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg
    21481 taaattctgt tcgaccgtca ggttaccgta gagcgaaaac ttttgcgcca tatagccgag
    21541 atgctggcgc gctttaccag aactctcttt cagatccatc cccagtacca gcgcctgacc
    21601 agaggtcggc accagcaaac cgcacatcat cttaaaggtg gtcgatttgc ccgcgccgtt
    21661 tggccccagc aaaccaaaaa tctccccgtg tttaacggca aagttgacgt gatcggtggc
    21721 ggcaaaatca ccaaatttct tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt
    21781 gccttctacc gtatgtaata ttgcgcccag cggcgattcc gaggttccgg caccgcccag
    21841 caaatcaata aacgcatctt caaaacgcgg cgtagtttcg ttgatgttga tttccggcat
    21901 cccgtcggca tggcgaatat cgtctggtgt ggtctctttt ttgaggatca gacgtaccga
    21961 tttcccctga atcatgccgt cgctgacctg cggcagtttc aatgcccgtt gcaacagttt
    22021 gcggttgccc tcgtgtggac ttgtcatcag aaagctgcgt ccggccatgg tttgtgtcag
    22081 ggcttttggt tccccctgat acagcaactc gccttcattc atcagtaaca cgtcacggca
    22141 ctgctcggct tcgtcgagat acgaggtact ccagaggatt aacatccctt cgcctgccag
    22201 ctcatgcacc atctgccaca gttcgcgccg tgagataggg tcaacgccga cgccgggttc
    22261 atcgagcagc aacactttcg gttcgcccac cagggtacag gccaggccga gtttttgttt
    22321 catcccaccg gagagcttgc ccgccaggcg tccggtaaac ggcccaagag acgtaaactc
    22381 cagcaggcga gcaaaagttt gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta
    22441 cagattgagg ttctccatca ccgtgagatc ttcatacaga ccaaatttct gcggcatata
    22501 gccgagcacg gcgtgcagcg cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc
    22561 attgccgctg tcgggtttca gtaatcccgc cagcatccgc attagcgtgg ttttacctgc
    22621 accatccggc cccaccagcc ccgtcacata accggcgtga atggtacaat cgagcggcgc
    22681 gacggcgggc ttgtccatgc ccggaaagcg tttttccagg ccgttcagcg tgataacggc
    22741 atcattcatg tcctgcctcg ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg
    22801 catcatcggc gtcggtcacc acaatacgca ggcgatagac gaggtcggta cgcagatccg
    22861 gtgtttcgac ggttttcggg gtaaattcag cagtcggcga aacgaaacca atctgcccgt
    22921 gatacggctt gtccgggcga ccatcggtat aaagcagcac tttgcgcccc ggctgggcct
    22981 ggtcaagata ccgttcatca acataagcgc gcacccacac cggacgcgtt agtgaaacgg
    23041 taaacaccgt gccaccttca ttgaggaccg tgcctggctc caccgcgcgc gttaacagcg
    23101 tgccatcaga cggggctatc aacgttgagt cctgtaaatt caactccgcc tgcgccagtt
    23161 gcgcctgcgc ctgttcgagg ctggctttcg cctgggcgat gtcctgttca cggttaccgg
    23221 aacggtactg acgcaattta tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg
    23281 aggagcgggc attttccagg tcatttgccg aaatagtgcg gcttttccac agtccttgct
    23341 ggcggttata gaagttctgc gcatagtcat aggcggcttg cgcctgtttc accgctgcgg
    23401 cggcctgagc gatttcttca tcgcgatacc cggcaagcat cagatcatac tgcgcctgtg
    23461 ccaccgagac gcccgctttc gcctgcatca gggcaatctc atacggcttg tgatccagtt
    23521 cgcccagcac ctggcccgct ttgatagcat caccttcgtc caccgccagc gattcaacgc
    23581 gccccccaac acggaaacta agattcaccg tacgaatatc cacgttgcca tacagcgtca
    23641 ggccgttatc ctggcggctt tgataccacc agtagcctcc ggcaaccacg gcggcaagta
    23701 ccactaccgc cagttcgatc acgacaggtt ttttcatcac tacaaactcc tttgcgataa
    23761 tccttgcaaa atcaggtcga tatgacaggt caccgtctgg ttgatcagtt cggtcttttc
    23821 ttcatcgaac gcggtccagc cggtacgtaa cagaattgtt tctttgccaa gacggaacgc
    23881 cagaatctca ccaatcaacg catgggtatg aaggatcatg cgggtgtcat tggcgtcgct
    23941 gccggtccag gcggcaatca ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc
    24001 gtgcaccagg tggtaggctg ccgtgggaga gagctgctca cgggagataa acttgctgag
    24061 gttgacggtg tcatcctggg tgagcagctt aatcatgttc ctgcaggcgc gaaggagcag
    24121 ttcacggatg gcagcccgat caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc
    24181 atgcggacgg aactgctcgc caataaaatc ggcaatccac tgggcgcagg cgaggtacaa
    24241 atcttctttc gaaccgaagt agtaggtgat ggcagcgata ttctgcccgg cttgggcggc
    24301 tatctcgcgg gtggtggcgt tcattccata ttcaccaaac tgcgccagtg cggcagcaat
    24361 cagctgtttt ttcgcctgtt cacccttgat tgtcatggca ggattattca tcgcacagtc
    24421 cattcttaat caaatgattg attaagatta tgactccata tgggagttgt ccagtatggc
    24481 taagaatttt agctacgcca gtcacaggga taatttatgc gctgcgtcac aaaaactgct
    24541 acactccgct cgctcatgac attgtggttt ttgtcatttt ccttttcagt atctccctga
    24601 aaactacacc ggtaacggtc ggggcggttc ggagtagtca tgtctttcga ttatttgggt
    24661 ttaagccctg atatcctgcg cgccgttgcc gagcagggtt accgtgaacc cacccctatt
    24721 cagcagcagg cgatccctgc ggtgctggaa ggccgcgacc tgatggctag cgcccagacc
    24781 ggcaccggca aaacagcggg ctttacgctg ccgctgttgc aacacctgat cactcgccag
    24841 ccgcacgcca aagggcgtcg tccggtacgt gcgctcattc ttaccccgac ccgtgaactg
    24901 gcggcgcaga ttggcgaaaa cgtccgtgat tacagcaaat acctgaacat tcgttcgctg
    24961 gtggtgtttg gtggtgtgag tattaacccg cagatgatga aactgcgtgg cggcgttgat
    25021 gtgctggtgg caacgccggg acgtttgctg gacctggaac atcagaatgc agtgaagctg
    25081 gatcaggttg aaatcctcgt cctcgatgaa gctgaccgca tgctcgacat gggctttatc
    25141 cacgatatcc gtcgcgtgtt aacaaaacta cctgcgaagc gccagaacct gttattctcc
    25201 gcgaccttct ctgacgatat taaagccctg gcggaaaaac tgttgcacaa cccgctggaa
    25261 atcgaagtgg cacgccgcaa taccgcgtct gatcaggtga cccagcacgt tcactttgtc
    25321 gataagaaac gcaaacgcga attgctgtcg cacatgattg ggaaagggaa ctggcagcag
    25381 gtgctggtgt ttacccgtac caaacacggc gctaaccatc tggctgaaca gctcaataaa
    25441 gatggcatcc gtagtgcggc gatccacggc aataaatcgc aaggtgcgcg tactcgtgcg
    25501 ctggctgatt ttaaatcggg cgatattcgt gtactggtgg caactgacat cgctgcgcgc
    25561 ggcctggata ttgaagagct gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa
    25621 gattatgtcc atcgtatcgg gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg
    25681 ctggtgtgtg ttgatgaaca caaactgctg cgtgatatcg aaaaactgct gaaaaaagag
    25741 atcccgcgca ttgcgattcc gggctatgag ccggacccgt caatcaaagc cgaaccgatc
    25801 cagaacggtc gccagcaacg tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa
    25861 cagcaaccac gccgtgggga aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa
    25921 ccgtctcgcc gcctcggcga tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg
    25981 cgtaaacctg ccgctgcgca gtaatctttt atgccgggct atgcccggca tcaggctgat
    26041 gaacaaacgc aaaactgcct gatgcgctac gcttatcagg cctacgtgaa ctctgcaata
    26101 tattgaattt gcatgctttt gtaggccgga taaggcgttt acgccgcatc cggcatttca
    26161 caacaagcac ttgtcagcaa tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca
    26221 taagccaccg agacgttact tctctatagc caattgttct cgcaactcca ttaaaaggta
    26281 gcccagtcga ttcttgccct taccattacc accgtctccc cagtaagcat cgttttccgt
    26341 atgctcaacc agttttgcgg gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg
    26401 ttcgaattta gcacgaagtg ctttacgcat cacctgttct ttaaccgact cccagttttt
    26461 acgaagtggc ttggaacgat tacgccccat gcgagcggcg accatggggg aagaaacccg
    26521 acggatctct tcgcggtatt tttcatcaag gaatttttgt gcctgaaaat agtgttcgga
    26581 ggtgggccag gttttcccgt caaccttgat gggccaggcg gcaaagttag aaaaatcacc
    26641 gtagtcatcg ctggtgctgt agaaatttat gatggtgtct tgcatgacgt gttggatcct
    26701 ttgtgctcga acgggcatta aaccgcatta tgttggtggt tattgcgagc cgctttccag
    26761 aaacagaaaa accattaccc ctgaaaaccg aaaaatgcca caatattggc tgtttataca
    26821 gtatttcagg ttttctcatg gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt
    26881 ataaggcgct tcaggaacag atccccgact ttattccccg tgtgccgcag cggcagatga
    26941 ttgcggacgt cgccaaaacg ctggccggag aagaaggccg gcatctggcg attgaagccc
    27001 ccaccggcgt tgggaaaacg ctctcctatt tgatccccgg catcgccatt gcccgcgaag
    27061 agcaaaaaac gctggtggtg agtaccgcca acgtggcgtt gcaggatcag atttacagca
    27121 aagatttacc gctgctgaaa aagatcattc ccgatcttaa attcactgcc gctttcgggc
    27181 gtgggcgcta cgtttgtccg cgtaatctga cggcgctcgc cagcactgaa cccacgcaac
    27241 aggatctgct ggcgtttctt gacgacgaac tgacggcaaa taatcaggaa gagcaaaaac
    27301 gttgtgcgaa gctgaagggc gatctcgaca cttatagatg ggatggtctg cgcgatcata
    27361 cggatatcgt tatagatgat gatctctggc gtcgtttgag taccgacaaa gccagctgcc
    27421 tcaaccgtaa ctgttactac tatcgtgaat gcccgttttt tgttgctcgt cgggagattc
    27481 aggaagcgga agtggtggtg gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag
    27541 ccgtactgcc tgacccgaaa aatctgctgc tggtgctgga cgaaggtcat cacctgccag
    27601 atgtggcccg ggatgcgctg gagatgagcg ctgaaatcac cgcgccctgg tatcgactac
    27661 agctggactt gttcacgaaa ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca
    27721 tcccaccgct ggcgatccct gaacgtttga atgcgcattg tgaagagttg tatgagctta
    27781 tcgcctcgtt aaacaacatt ctcaatctct acatgcctgc cgggcaggag gcagagcacc
    27841 gttttgcgat gggcgaactg cctgatgaag tgctggagat ctgccagcgg ctggcaaaac
    27901 tcaccgagat gctgcgtggt ctggcggagt tatttcttaa cgatttaagt gagaaaaccg
    27961 gcagccatga cattgtacgc ctgcatcggt tgattttgca gatgaaccgc gcgttgggga
    28021 tgttcgaggc gcaaagcaaa ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc
    28081 cggtgaccaa atgggcgacg cgggaagagc gcgaagggca actgcatctc tggtttcact
    28141 gcgtggggat acgtgtcagc gatcagctgg aaaggctgct gtggcgcagt attccgcaca
    28201 ttattgtcac ctccgcaacc ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga
    28261 gtggtctgaa agagaaagcg ggcgaccgtt ttgtggcgct ggattccccc tttaaccact
    28321 gcgaacaggg caaaattgtt attccccgga tgcgcgttga gccttccatc gacaacgaag
    28381 agcagcatat tgccgaaatg gcggcctttt tccgtaagca ggtggagagc aaaaaacatc
    28441 tcggtatgtt ggtactgttt gccagcggac gggcgatgca gcgctttctc gactatgtga
    28501 cggatttacg tctgatgttg ctggttcagg gcgatcagcc gcgttaccgt ttagttgaac
    28561 tgcaccgcaa acgcgtcgcc aacggtgagc gcagcgtgct ggtgggctta cagtcatttg
    28621 ccgaagggct tgatttgaaa ggtgatctgc tcagccaggt gcatatccac aaaatcgctt
    28681 ttccgcccat cgacagcccc gtggtgatca ccgaagggga atggctgaaa agcctcaacc
    28741 gctatccgtt tgaggtgcaa agcctgccga gcgcctcgtt taacctgatt cagcaggttg
    28801 ggcgactgat tcgaagccac ggttgctggg gcgaagtggt tatctacgac aaacgcttgc
    28861 tgaccaaaaa ttatggcaag cgactactgg atgcattacc ggtatttccg atagagcaac
    28921 cggaagtccc tgaaggtata gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc
    28981 ggcgttaatg atgtgagtca ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag
    29041 agatcgggcg cgggaaaaac cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt
    29101 atgctcatat gctcaatggt gaagtccctg accttgagtt gggcggcgta ttgattgcgc
    29161 tgcgtatcaa aggagaaggg gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc
    29221 acaccatcaa gctgacgccg ccagcaggca agccgatgcc gattgtcatc cccagttaca
    29281 acggcgcgcg taaacaggcc aacctgacgc cgttgttggc gattctcctg cacaaactcg
    29341 gttttcctgt ggtggttcac ggggttagcg aagatccaac ccgcgtgctg actgaaacca
    29401 tttttgaatt gatgggtatt acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg
    29461 acgaacatca accggtgttt atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac
    29521 tggcgatgcg ctggcggatg ggcgtgcgta acagtgcaca taccctggcg aaactggcga
    29581 cgccatttgc cgaaggtgcg gcgctgcgtc tttccagcgt ttcgcatccg gaatatattg
    29641 gacgcgtcgc gaagttcttt agcgatatcg gcgggcgggc gctactaatg catggtactg
    29701 aaggtgaagt gtatgctaat ccgcagcgct gcccgcagat caatctcatt gaccgtgaag
    29761 ggatgcgggt gctgtatgaa aaacaggaca ctgctggtag cgagttactg ccacaagcaa
    29821 aagatccgga aaccacggcg cagtggattg agcgttgcct tgctggcagc gaaccgattc
    29881 ccgaatcgct gaaaatccag atggtttgct gcctggtggc tacgggtgaa gcggcaacta
    29941 tcagcgacgg cctggcgcgc gttaatcagg cattttaatt cttttctcag ccggatgagc
    30001 catgtcatcc ggcttttacc ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc
    30061 cacgcccggc gtgcagcata aaaatacaac aaacacataa cataaacagg agttaaccat
    30121 ggaaagtggt catcgctttg atgctcagac gctgcacagt tttattcagg ctgtatttcg
    30181 tcagatgggt agcgaggaac aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa
    30241 cctggcaggg catgattcac atggtattgg catgatccca agctatgtac gctcctggag
    30301 tcaggggcac ctgcaaatta accatcatgc caaaaccgtt aaagaggcgg gggcggcggt
    30361 cacgctcgat ggcgatcgcg catttggtca ggtcgcggca catgaagcga tggcgctggg
    30421 gattgagaaa gcgcatcagc acggtattgc cgccgtggcg ctacataact cgcatcatat
    30481 cggccgtatc ggttactggg cggagcagtg tgcagcggcg gggtttgtct ctatccactt
    30541 tgttagcgtg gtcggtattc caatggtcgc gccgttccac ggtcgcgaca gccgctttgg
    30601 caccaatccg ttctgtgtgg ttttccctcg taaagataat ttcccgctgt tgcttgatta
    30661 cgccaccagc gccattgcat ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt
    30721 gccgccaggt tgcctgattg acgttaacgg cgtgccgacg accaatccgg cggtaatgca
    30781 ggagtcgccg ttgggttcgc tgttgacctt tgccgaacat aaaggctacg cccttgcagc
    30841 gatgtgtgaa attcttggcg gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt
    30901 acaaaccagt cccgatgcca ttcttaactg catgaccact atcatcatca acccggaact
    30961 cttcggcgcg ccggattgta acgcgcagac cgaagccttt gccgagtggg tgaaagcctc
    31021 gccacatgat gatgataagc cgattttgct accgggcgag tgggaagtga acacgcgtcg
    31081 cgaacggcag aagcagggga ttccactgga tgcgggaagc tggcaggcca tttgtgatgc
    31141 agcgcggcag attggtatgc cggaagagac gttgcaggct ttctgtcagc agttagccag
    31201 ctaaaaaaaa gcccgtccag tggcggacgg gcaaacaagg gtaacatagg atcaatgagg
    31261 gttagagcat atgcgtctgt cggcaaacag acagggaaat acttgtgctg gacgtagcgt
    31321 aaacgcctga tccgtccaac ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg
    31381 catcaggcat ctgttgccgg atgcggcgta aacgccttat ccgtcctacg aattacttat
    31441 aaattaccgc agtaccgctt aatttattgt tgttggtggc ggaagtgatg ctgtatccac
    31501 tggcaccggc tgccgcggct ttctcagcca gtttcgcttc cagggcatcg agggtggatg
    31561 cgccatcggc agaaaccacg ccgattttat tcatgttctg tgcctgggat gccgttatcg
    31621 gttccgccgc gaacacgcca aatgacagag ttgaaagagc catagcagca acaacagtat
    31681 tgatagtttt cataattaat ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc
    31741 gatgtgatga gtatcacgtt tttttacgag agataaaatc gaagagaatt gaccgccttg
    31801 ttcaaataaa ttgattgata tctaacatac tgataaacca tttatttgtt gtacttatga
    31861 acttgtttta taccgcgtgg cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa
    31921 taaaaattat actcgctatg catctcgcca gttgtagtta acgtaactgg ctgtctttcg
    31981 aacctctgcg attatatcct gtataagctg gtttttgtaa atctttctcc tgctgacaat
    32041 gaatacataa gcgcacgcca ggaatggctt cccgacgggc ctgcgggatg ggggcaccgc
    32101 actcttcaca ttcatccagg ctttcgccgc gcggaatttc accccgagcg cgggcaatcg
    32161 catcttcaat tgtactgttg atctgttcgt tgacggcgtc atcgttagcc caaccggatg
    32221 ccatatcgac ctccccatat caatacttgt acagttaagt gtagctaatc cagggacgaa
    32281 ctcgggcagt tcaagcatca gatctccgac cattcccgca gcagattatg ataaagatta
    32341 agcagcgaca ggatctcttc actttcgccg tagcggcttt tcagcgactg aatattgttg
    32401 tccagttcaa acagcatggc gcgcttttta tcatcgcgga tcatcgactg gatccacata
    32461 aatgatgcca ctcgtacgcc gcgggttacg ggtggcacgc aatgctggct gctggaggga
    32521 tacaacacga gatcgcctgc cgggagtttt acccgatgtt gtccgaaggt gtcattaacg
    32581 accagttcgc cgccgtcgta gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca
    32641 gtacgcatcc agccgttttg tggatggctg cgtactgcgc catccacatg gaaaccatag
    32701 gtttcattgt tctgatagcg attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg
    32761 gcaaagaaca aagcatgttg gttaaccgcg ttcagcacct cattttgcag ggcggcgtat
    32821 aacgtgctgc gggtgtcgac ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg
    32881 gtgacgcgtc catccaccca ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgtcc
    32941 tgtggcgata acacgccggg aatgtggtac atcatacaag ttctccaaaa agtggggcct
    33001 gcgccccaca tctgaatcag aaatgcatat tggctgtgag caagaaggtt cttggctcgc
    33061 ccgggtgata acggtagccg ctcttgttga ttgaggcgac gtaatcagta tcaaacaggt
    33121 tgtagacgtt tagctggaag tcgagattgc gattaacccg ataccccagt ttggcatcgg
    33181 cgacccagta accttcggta aatgctggcg ttcccaccgc gccgtctgaa cctttatgca
    33241 tactgccgat atagcgtgcg cccgcgccaa cagagatatc gtcggttgcc tgatattggc
    33301 tccataaggt gaaggcgtgc tccggggtat acggcagcga tgaggaacca tcctgggcaa
    33361 catctttgcc gtttttgatg gttgcttttt gctgggtata gccgccaatc acctgccacg
    33421 cgggagtgat attcccggcc acggatatct catagccttc gacgcgtttc ttaccgtatt
    33481 gcgagtaagt tccgtcatca ttttgctcaa cttcattttc gatatcagta cggaacagcg
    33541 cggcggtgag caacagacgt ttatccagaa cctgccattt ggtgccaatc tcgctggtgt
    33601 tggctttttg cggtttaaaa tcggtgcggt tggcactgtt accgctgcca gactgcgcaa
    33661 gggcgaagtt gttgccgccc ggaggctgct gggaaacggc atagttaata tagacattgc
    33721 cgttttccgt caggtgatac agcgccccgg ctttccagtt catcagattg cccgacttgg
    33781 cggtgtcgac ggtggtgacc ggagaacctt ttgccacacc agttgggcag gtgatggcac
    33841 cgcgtccgct gccgccgcag gcggtggcac tgtcatattc agtatgataa ttatccagac
    33901 ggatcccgcc gttcagctca aaatcacggg tgatttgcag cgtatcaaag gcgtaaattg
    33961 cgaaggtatc cgtctgacca ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa
    34021 tgctgctgtc aggatgataa atatttaccg cgggtaacgt caccggatta acgccgtagt
    34081 tagtctgcgt ttcacgggta aattccacgc cggtactgac atcatgaccg atagaaccgg
    34141 tatagaacgt cgaggtcagg ttggtctggt tggtgagaat tttattactc acatctttgg
    34201 tattcgccgt gcgtgaccag gttcagctat tcacatcgct ggtgggctga gtaatattcg
    34261 acgccccgcc cataatcgcc gtcatcaggt aatcctgctt tacgcgcgac caacgggtag
    34321 tattgcgaat ggtggtgtta tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg
    34381 tggtcgaatc gtcgtaatcg gaatccgtgc cgtaaaagtt atgagtatca acttttccgg
    34441 aatgattcag ggccgccgtt cccgcagatg gggcagaata gcccggcaaa ccgatggtcg
    34501 gaatgccgcc gtctggcgtg ttgtgctggg tgacatgcag ataattaaga tacaaacgat
    34561 tcgctgtacc aaggccaaaa gcgacagaag gggcgacgcc gtaacgctca tttttgactt
    34621 tgtcgcgtcc ggcatcgtgc gttttttcgc ccattacatt caggcgcacc gcggtggtat
    34681 caccaatgac ctgattgacg tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg
    34741 cggaggcgtc aatgccggaa tcattgcgcg gctgtttgct gatcatattg atcgagcccg
    34801 tcggtgcgct gcgcccgtag tcggtgccgg acggcccttt aatcacttcg acctgctcgg
    34861 tattgaaggt gtcgcgcgag acgctgccga tatcgcgaat gccatcaata taaatactgt
    34921 tagaggtatc ggcaccacgc atataaatgg cgtcgccagt ggtggagtta ccgttctcac
    34981 ccgcaaaaaa cgcacccacg ccgg
//
LOCUS       DAJVRF010000029        34000 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJVRF010000029 DAJVRF010000000
VERSION     DAJVRF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34000
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.029"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            13..515
                     /locus_tag="OQN13_001288"
                     /pseudo
     CDS             13..515
                     /locus_tag="OQN13_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(582..2039)
                     /gene="nuoN"
                     /locus_tag="OQN13_001289"
     CDS             complement(582..2039)
                     /gene="nuoN"
                     /locus_tag="OQN13_001289"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCS1614690.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2046..3575)
                     /gene="nuoM"
                     /locus_tag="OQN13_001290"
     CDS             complement(2046..3575)
                     /gene="nuoM"
                     /locus_tag="OQN13_001290"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCS1614691.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3739..5580)
                     /gene="nuoL"
                     /locus_tag="OQN13_001291"
     CDS             complement(3739..5580)
                     /gene="nuoL"
                     /locus_tag="OQN13_001291"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCS1614692.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5577..5879)
                     /gene="nuoK"
                     /locus_tag="OQN13_001292"
     CDS             complement(5577..5879)
                     /gene="nuoK"
                     /locus_tag="OQN13_001292"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCS1614693.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5876..6430)
                     /gene="nuoJ"
                     /locus_tag="OQN13_001293"
     CDS             complement(5876..6430)
                     /gene="nuoJ"
                     /locus_tag="OQN13_001293"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCS1614694.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6442..6984)
                     /gene="nuoI"
                     /locus_tag="OQN13_001294"
     CDS             complement(6442..6984)
                     /gene="nuoI"
                     /locus_tag="OQN13_001294"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCS1614695.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(6999..7976)
                     /gene="nuoH"
                     /locus_tag="OQN13_001295"
     CDS             complement(6999..7976)
                     /gene="nuoH"
                     /locus_tag="OQN13_001295"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCS1614696.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7973..10699)
                     /gene="nuoG"
                     /locus_tag="OQN13_001296"
     CDS             complement(7973..10699)
                     /gene="nuoG"
                     /locus_tag="OQN13_001296"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCS1614697.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10752..12089)
                     /gene="nuoF"
                     /locus_tag="OQN13_001297"
     CDS             complement(10752..12089)
                     /gene="nuoF"
                     /locus_tag="OQN13_001297"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCS1614698.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12086..12586)
                     /gene="nuoE"
                     /locus_tag="OQN13_001298"
     CDS             complement(12086..12586)
                     /gene="nuoE"
                     /locus_tag="OQN13_001298"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCS1614699.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12589..14391)
                     /gene="nuoC"
                     /locus_tag="OQN13_001299"
     CDS             complement(12589..14391)
                     /gene="nuoC"
                     /locus_tag="OQN13_001299"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCS1614700.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14485..15147)
                     /gene="nuoB"
                     /locus_tag="OQN13_001300"
     CDS             complement(14485..15147)
                     /gene="nuoB"
                     /locus_tag="OQN13_001300"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCS1614701.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15163..15606)
                     /gene="nuoA"
                     /locus_tag="OQN13_001301"
     CDS             complement(15163..15606)
                     /gene="nuoA"
                     /locus_tag="OQN13_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCS1614702.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16235..17173)
                     /gene="lrhA"
                     /locus_tag="OQN13_001302"
     CDS             complement(16235..17173)
                     /gene="lrhA"
                     /locus_tag="OQN13_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCS1614703.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPYLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18088..19305
                     /gene="alaA"
                     /locus_tag="OQN13_001303"
     CDS             18088..19305
                     /gene="alaA"
                     /locus_tag="OQN13_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCS1614704.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19389..19988
                     /gene="yfbR"
                     /locus_tag="OQN13_001304"
     CDS             19389..19988
                     /gene="yfbR"
                     /locus_tag="OQN13_001304"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCS1614705.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVNMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20047..21879)
                     /locus_tag="OQN13_001305"
     CDS             complement(20047..21879)
                     /locus_tag="OQN13_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCS1614706.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(21966..22614)
                     /gene="hxpA"
                     /locus_tag="OQN13_001306"
                     /pseudo
     CDS             complement(21966..22614)
                     /gene="hxpA"
                     /locus_tag="OQN13_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(22625..23119)
                     /locus_tag="OQN13_001307"
     CDS             complement(22625..23119)
                     /locus_tag="OQN13_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCS1614707.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23202..23657)
                     /locus_tag="OQN13_001308"
     CDS             complement(23202..23657)
                     /locus_tag="OQN13_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCS1614708.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            23995..25197
                     /gene="ackA"
                     /locus_tag="OQN13_001309"
     CDS             23995..25197
                     /gene="ackA"
                     /locus_tag="OQN13_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCS1614709.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25272..27416
                     /gene="pta"
                     /locus_tag="OQN13_001310"
     CDS             25272..27416
                     /gene="pta"
                     /locus_tag="OQN13_001310"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCS1614710.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            27606..29126
                     /gene="yfcC"
                     /locus_tag="OQN13_001311"
     CDS             27606..29126
                     /gene="yfcC"
                     /locus_tag="OQN13_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCS1614711.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGTRMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29159..29701)
                     /gene="yfcD"
                     /locus_tag="OQN13_001312"
     CDS             complement(29159..29701)
                     /gene="yfcD"
                     /locus_tag="OQN13_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCS1614712.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(29759..30310)
                     /gene="yfcE"
                     /locus_tag="OQN13_001313"
     CDS             complement(29759..30310)
                     /gene="yfcE"
                     /locus_tag="OQN13_001313"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCS1614713.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30366..31010)
                     /gene="yfcF"
                     /locus_tag="OQN13_001314"
     CDS             complement(30366..31010)
                     /gene="yfcF"
                     /locus_tag="OQN13_001314"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCS1614714.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31146..31793
                     /gene="yfcG"
                     /locus_tag="OQN13_001315"
     CDS             31146..31793
                     /gene="yfcG"
                     /locus_tag="OQN13_001315"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCS1614715.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLTEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            31850..32212
                     /gene="folX"
                     /locus_tag="OQN13_001316"
     CDS             31850..32212
                     /gene="folX"
                     /locus_tag="OQN13_001316"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCS1614716.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32233..33126
                     /locus_tag="OQN13_001317"
     CDS             32233..33126
                     /locus_tag="OQN13_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCS1614717.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVFISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33174..>34000)
                     /gene="rpnB"
                     /locus_tag="OQN13_001318"
     CDS             complement(33174..>34000)
                     /gene="rpnB"
                     /locus_tag="OQN13_001318"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCS1614718.1"
                     /translation="TARDFIDIHLPAPLRKLCDLTTLKLESNSFIDEDLRQYYSDLLW
                     SVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAAMQNHLDAGYKELPLVIPMLFYH
                     GCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDITVVPDDEIMQHRKMALLELIQKH
                     IRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQTGDAQRFRAFIGEITERAPQEK
                     EKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         8481 a         9279 c         8563 g         7677 t
ORIGIN      
        1 gaggaaaaat aaatgggaat gattggttat tttgcagaaa ttgactcaga aaagattaac
       61 caactgcttg agtctactga aaaacctttg atggataata tacatgacac gctttcagga
      121 ttacgccgac tggatattga taaacgctgg gattttttac actttgggct gacaggcacc
      181 cctgcttttg atcccgcgaa gaatgatccg ctgagtcgtg ccgtgcttgg tgaacacagc
      241 ctcgaagatg gcatagatgg cttcctggga ttgacctgga atcaggagtt agccgcgaca
      301 atcgaccgac tggaatcact ggatcgaagt gagctgcgta aacagttttc cattaagcga
      361 ctcaatgaaa tggagattta tccgggagtg accttttcag aagagttaga ggggcaactc
      421 tttgcttcga tcatgctgat atggaaaagc tgattagcgc gtatcgacgt atgctgcgac
      481 aagggaatca tgcgctgacg gttattgttg ggtgatggtg tcagggcacc aaaaaaaacc
      541 gccgaacatg tcggcggctt tctgacttac aaactgatag cttacatcag cggcattgct
      601 aaccgcacaa tgctaatcag cggttgtggc catacaccca gcaccagtac caacagtgca
      661 gagatcagca ccacgatacc gcctgcgctg tactgccagt ttgacggtgc atcgcgaccc
      721 ggttgttccg gggcgtgcag gtacaggctc accgccacgc gcaggtagta gtaaaggccg
      781 cttgccgaac cgacaaccac ggcgcccacc agccaccaca agtgtgcctg gacaccgacc
      841 gccagcacgt agaacttacc aataaagccc agcgtcatcg gaataccggc cagagacagc
      901 atcatcaccg tcatcaccgc cgcgaggatc gggcgatgcc agaacagtcc gcggtaagag
      961 tacagggaat cagcatccgg gccacgatac gggctggaca tcaggctgac cacgccgaac
     1021 gcgccgaggc tgctaaacag ataaccggcc aggtaaaccc ctaccgcttc catcgacatc
     1081 tcgccagttt gcagcgcaat cagcgccacc agcagatagc cgaggtgaga gatagatgag
     1141 taaccgagca gacgtttgat attggtctgg ctcagcgcca tcaggttacc gaagatgatg
     1201 gaggcaaagg cgataatcgc cagcaccacg cgaatcgctt cgctgtcacc catcggtgcg
     1261 tagaggaaca gacgcatcac cacaccgaag atagcgattt tgctcgccgt cgccaagaaa
     1321 gtggaaaccg gcgcaggcgc gccctggtat acgtctggcg tccacaggtg gaacggcacc
     1381 agagagagtt tgaagccgag gccaacaatc atcaggccga aacctgccag caacagcggc
     1441 tcgttgagca taccgtcgcc aaggttttta cccaacgcga caaacgacag gtcgccagac
     1501 tgcgcataca ccagcgccat accaaacagc aggaaagaag acgctgcggc agaaaggatg
     1561 gtgtatttga tactggcttc cagtgaacgt ttctggcgga aagcgtaacc gaccaggcca
     1621 aacagcggca aagagatcag ttcgataccg aggaacagag acgccagatg gttggcattc
     1681 gccagcagga tcccgcccag cgcggcaatt aacaccaaca ggtagaactc gtccttgttg
     1741 tcgttatagc cttcaagcca cgggtaggcg aaagtacagg tggcgaggct cgccaacaat
     1801 accagcccgg tgtaaagcat ggcgaaacca tcaacgcgca tcagcggcgt aacgtccata
     1861 gcgcctgcct ggccgacaaa ccagagcgaa accagcgccg cgttaagccc aataaccgag
     1921 agcgtagcgt tgaggaaatg attgcgtcgc cacgcaatgg agagcatcac aaccaccacc
     1981 gtcaagccga cgatcagcaa cggtagcagt gcgatcaggt tttgtggagt tattgtcatg
     2041 gcgatttacg gccttgtagt agtaacggaa ttaacaaacc actgctggat attgccaatc
     2101 gcggagtgcg aggtatccag aatcggctgc ggatagaagc cctgcagtac cagcagcacc
     2161 accagcaaca ggatcataaa cagctcacgc agcgacatcc ctggcagttc ctggctggca
     2221 atctcgcttt tcgctttacc gaagtaagcg cgatgcaaca tcgccagcga ataaacagat
     2281 gcaaagacca gcccaaaggt agagataacg gtaatcaccg ggacaacctg gaagctgccg
     2341 aacagaatca taaattcgcc gacgaagtta ccggtgccag gcatcccaag cgttgccacc
     2401 gcaaagaaca gcgacagtgc tggcagccat ttcatcttgc tccacagacc gcccatcatg
     2461 cgcatgtcgc gggtatggat acgttcataa agctgaccac aaagaataaa cagacccgcc
     2521 gccgacaagc cgtgcgcaat catctggatt accgcgccct ggtaggccaa ctggctgccg
     2581 gtgtagatag caatcagcac gaagcccatg tgggaaaccg aggtgtaggc gatcagacgt
     2641 ttgatatcgg tctgggcgaa ggccatccac gcaccgtaga agatgccgat aacacccagc
     2701 cacatagcaa ttggcgcgaa ctctgccgac gcgttcggga acagcggcag ggagaaacgc
     2761 agcaaaccgt aagcggcagt tttcagcaag atccccgcga ggtcaacgga accggcggtc
     2821 ggagcctggg agtgcgcatc cggcagccag ccatgcagcg gaaccaccgg cattttgact
     2881 gcgaaggcga tgaagaagcc cagcattaac aggtattccg caccactgga cattggcgta
     2941 ttcagcagct cttcatagtt gaaggtccag acgccggtcg cattgtagtg aacaaaaacc
     3001 agcgccagga tggcaatcaa catcaccaga ccactcgcct gggtgtaaat gaagaacttg
     3061 gttgccgccg tgatacgcgt tttaccgtca gaggctttat gcccccacag tgcgatcagg
     3121 aagtacatcg gcaccagcat catttcccag aagaagaaga acaggaacat gtcgatggca
     3181 aggaacacgc cgataacgcc gcccaggatc cacatcaggt tgaggtggaa gaagccctga
     3241 tatttttcga tctctttcca cgaacatagt accgccagca caccgagcag accggtcagc
     3301 acgaccatca gcagcgacag cccgtcaatg gcgagatgaa tagagatacc caaacgcggg
     3361 atccacggca tgtcgaattc agactgccac tgcggaattc cggcggattg cgtcagtgaa
     3421 taaccgccct gcaaccacag ttgcagcgac agcgccagcg tcaatcccat ggtgatcagc
     3481 gcgatccagc gtggcacctt gacgccaaag cgttcggtct gccagcacag gaagccgcca
     3541 ataaagggaa ttaatattag ccagggtagt aacatggcga tctttattcc ttgtaaaagt
     3601 cccgtcagga ccggattttc aacgaattct cacgacagaa aattctcttc ggactggggg
     3661 tagtgtcgga tgcaccgcga aggcggctta tccgacctac gttcttatat gcccaggggc
     3721 aatcccacaa tccttaactc aacgcagtac catcaacagt gccagcacca cgaccgcacc
     3781 gatgctcatg gatgccacat accagcgcag atagccgttc tcacttaaca gcagaccttt
     3841 acctgcaaag cgggaaagga cagccggaat gttcatcatt gagttcagcg gatcgcgttt
     3901 cagcaaccag gcaataccca ggaacggcct gacgaacact ttgtcgtaca gccagtcaaa
     3961 gccccaggcg ttgtaccacc aggtactcag caggcggccc ggcgcactgt tggcgatgga
     4021 ggtcaccaga gtacgtttac ccagccacag ccaagctgcc agcagaatgc cgacaatcgc
     4081 gaccacgcca gaggtaattt ccagggtcaa catgctgccg tgcgccagtt ccgtcgtttg
     4141 cggaagcacg ccctgcagcg gcggtacaat cagtgcgcca acgaaggtgg aaaggatcag
     4201 cagcacaatc agcggcaggc tgtgagttac ccctttcacg gcgtgagcgt gaatttgttc
     4261 ttttccgtgg aagacgatga aaatcatacg gaaggtgtag agcgaggtca taaacgcacc
     4321 gaccagacct gccaccatca gattgatatg accattcgcc atcgcacccg cgaggatctc
     4381 atccttactg aagaagcccg cagtgaccag cggcagtgcc gacagtgctg cgccgcccac
     4441 caggaagcag agataaacca gcggaataga tttacgcaga ccgcccatct tgaagatgtt
     4501 ctgttcgtga tggcaggcca gaatgacgga accggatgcc aggaacagca gcgctttaaa
     4561 gaacgcgtga gtcatcaagt ggaaaatcgc cgcatcccat gcctgcacgc caagcgcgag
     4621 gaacatgtag ccaatctggc tcatggtaga gtaagcgaga acacgtttga tgtcggtctg
     4681 taccagcgcg gcaaaaccgg ccagcagcag cgtaaccgcc ccgacaatgc ccaccagatg
     4741 cagaatttcc ggcgtcatca ggaacaggcc gtgggtacgg gcgatcaggt agacacccgc
     4801 ggttaccatg gttgcggcgt ggatcagcgc ggagacaggc gtcgggcccg ccatcgcgtc
     4861 ggcaagccat gtctgcaacg gcaactgcgc agatttaccg accgcaccgc ccagcagcat
     4921 cagcgtcgcc cacatcagca tgttattgcc gtcagcaaag tgcgctggtg ccagttccac
     4981 catttcgcgg aagttcaggg tgcccagttc gttgtaaaga atgaacagtg cgaaagcgag
     5041 gaaaacgtca cccacacggg tcacgacgaa cgctttcatt gccgctgcgc cattcttcgg
     5101 atcggtgtaa tagaaaccga tcagcagata ggagcacagg cccacgcctt cccagccgag
     5161 gtacatcagc agcaggttgt cggcaagcac cagaactacc atgctggcga tgaacaggtt
     5221 ggtgtaggcg aagaagcgag agtagccctc ttcaccgcgc atgtaccagg aggcgtacat
     5281 gtgaataagg aaacccacac cagtgaccac cgagagcatg gtcagcgaca ggccgtccag
     5341 caccaggtta aaaccgatgt taaagtcgcc taccgacatc cacgtccaca gcggctggct
     5401 gtatgcctgc tcgccgttag cgaagaaatc aacgccgata aaggcggtta ccagcgccgc
     5461 caggcccaca gagccaacgc cgacgatcgc cgaaacgttt tcagaccagc gcccacggga
     5521 gaacgccagc aggacgaagc caatcaatgg caaaataatg gttaaggcaa gcatgttcat
     5581 ccgcgcatct cacttactga atcgatgttc aggttctggc gacgacggtg aagttgcagc
     5641 agcagcgcaa ggccgatact cgcttctgcc gccgcgaggc tgatggcgag aatgtacatc
     5701 acctgaccgt cggtctgacc ccagtagctt ccggcgacaa caaaggccag cgcggaggcg
     5761 ttaatcatga tttccagacc aatcagcata aacagcagat tgcgacggat aaccagaccg
     5821 gttaagccaa gaatgaataa gattgccgcg aggatcagtc catgttgtaa ggggatcatg
     5881 cgtgctcctc cgtttttctt ttcgcgctgt cgtctttacg attgctcagc acttcacccg
     5941 cacgctcttc acgaccgacg tggaaggcca caaccagacc tgcgagcagc agcatagaag
     6001 ccagttccac cgccagtacg taaggcccga acagcgtaat acccactgct ttagcactga
     6061 ttggcgtacc gtcgataccc tgatcgttaa caccgaggat ggcgtaaaca atcaccacca
     6121 gcatgatggc cgacaaaatt gccggaccaa tccacacctg cggtttcagc cactggcgtt
     6181 cctgttcgat ttctgaaccg cccaggttga gcatcatcac cacgaacacg aacagcacca
     6241 taatggcacc cgcgtagacg ataatttcca gcgcaccggc gaagtaagcg cccagtgaga
     6301 agaacacccc ggaaatcgcc agcagcgaaa taatcaggta cagcagtgcg tgtaccggat
     6361 tggtatgggt gatcactcgc aaggtcgcaa gtatggctat caggccacag atataaaaag
     6421 cgaactccat tgcccctctc cttacggtaa caggctcttg acgtcgatag gcttggcttc
     6481 gttctctgct tcgcccttat ctttgccgtc gattgccata cctgccatcc ggtagaagtt
     6541 atattccggg tatttgcccg gaccggagat cagcagatcc tctttttcgt aaaccagatc
     6601 ctggcgcttg tattccccca tttcgaaatc cggggttaac tgaatcgccg tggtcggaca
     6661 ggcttcttcg cacagaccac agaaaatgca gcgtgagaag ttgatgcgga aaaattccgg
     6721 ataccagcga ccgtctttgg tttctgcttt ttgcagcgag atacagccga ccgggcaggc
     6781 taccgcgcag aggttacagg ctacgcaacg ctcttcgccg tccgggtcgc gggtcagaac
     6841 gatacgacca cgataacggg gcggcagata gatcggctct tccgggtaca ttcgcgtttc
     6901 gcgtttggcg aacgcgtgca ggccgatcat ccagatacta cgaacctggg tgccgaaacc
     6961 tactaacaat tcttttaagg tcatggtctt attgcccctt attgcgcctg ccagagaatg
     7021 acagccgccg ttaccagcaa gttgatcagc gtcagcggca ggcagatttt ccagccgaag
     7081 gacattacct ggtcataacg cggacgcggt aacgacgcac gaatcaaaat gaacatcatc
     7141 ataaagaacg cggttttcag cgcgaaccag atgaatggcg gtaacaacgg gccttgccag
     7201 ccaccgaaga acagcgtcac catcaatgca gagatggtca caatcccgat gtactcaccc
     7261 acgaagaaca gaccgaactt cataccggaa tattcaatgt ggtaaccatc cgccagttcc
     7321 tgctcggctt ccggctggtc aaacgggtga cggtgacata ccgccacgcc cgcgatggca
     7381 aaggtaataa aaccaaagaa ttgcgggata acgttccaca catgcgcctg gctgttgacg
     7441 atgtcggtca tgttgaatga accggcctgc gccaccacgc ccatcaggga aagcccgagg
     7501 aacacttcgt agctcagggt ctgcgcagaa gcacgcatcg cacccagcaa cgagtatttg
     7561 ttgttacttg accagcccgc aaacagcacc gcgtaaaccg ccagacctgc catcatcagg
     7621 aagaacaaaa tcccgatgtt caggtcggca accacccaac ccggactgac tggcacaatc
     7681 gcaaaggcca gcagcagcga ggtaaaggca atcatcggtg ccagggtaaa gatgacgcga
     7741 tccgagaatt tcgggatcca gtcttcttta aagaacattt tgatcatgtc cgcaaccagc
     7801 tggagcgaac cgccccagcc aacacggtta ggtccgtaac ggttctggaa cagacccagc
     7861 aggcgacgtt cgccaaagct catgaatgcc ccgcaggtga caaccaccag caggatcacc
     7921 accgctttga ggatggtcag caggatctca atcagttccg gtgatatcca actcattgtt
     7981 gtgcctcctt gagatcctca agatgcgcgc cagccagcac cggagcaata ccggacatac
     8041 ccatcggcaa gcccacctgc cctgccgtca gcccttcagc gatttcaacc ggcagcgtga
     8101 ccgtattgcc atcgtaacta aaggagacgt gtgtacctgc gttcacacct aacttcgcgg
     8161 catccgctgg gttgagtttg atgtacggct gcggcatacg gctctggaag accggagcac
     8221 gctgtgacaa ttcatcgctg ccaaacaggt ggtaatacgg cgcgatacgc catttcccgt
     8281 cctgcggctg gaagcgtgcc ggtacgctgg tgaagtaatc cagaccattt tcgctggttt
     8341 caaacagacg cacgcccgga tcgccaaagc gcagtttgcc gcccacttcg tcctggaatt
     8401 tgttccacgc ctgcggggag ttccagcccg gcgcccaggc aaacggcact tgcgaacggt
     8461 gcgcagtcgg ctggttgtta ccttccatcg agaaggtgaa catggtgtca atatcctgcg
     8521 gctgacgcgg ctcatgaacg ctgatattgg cgcgcatggc ggtacgaccg ctgtaacggt
     8581 gcggttcacg ggccagtttc tgcccacgaa tacggaatgt cgcatccggc gcagcatctt
     8641 tgatacctgc cagttccggg attttcgcca caacagcgtc aatcacatgg tcgagctgcg
     8701 tccagtccac ttcacggctc agcagggtgc tgtgcagcga gtgtaaccag cgccagcttt
     8761 ccagcatgac agttttgctg tcgtaataag caggatcgta aacctggaag aaacgttggg
     8821 cgcggccttc gttgttgatc accgtaccgt cgctttcagc aaaactggca gcagaaagta
     8881 ccagatgggc gttttccata atcgctgtgc gttgatgatc aaccaccatc accagcggcg
     8941 ctttagccag cgcagcattc acgcgggtag cggaagcgtg acgatgcaga tcgttttcca
     9001 gcaccaccac cgcgtcggcg cgtccggttt ccagttcggt caacgcttct tcaagcgaac
     9061 cgccacccat aatgcccagc cccatgctgt tgacggaacg ggcaatcatg gtgataccaa
     9121 cgtcagcgcc gcgacctttc agggctttcg cgacgttagc cgcagcctga atcacctcta
     9181 tgctaccggc gttcgtcccg gagataatca acggtttctt cgcacctgcc agtgcctgca
     9241 cgatgacgtc gattttgctt tgcagctcag gttcgatacc gtcaaccgct ggcgcagagt
     9301 tatccagcgc atgggcgatg gcaaaaccta aacgcgcctg atcttcaacc ggtgcgcggt
     9361 aagtccacgc cgcgatatca tccagacggg tgtcatcaac gttggtaaca aacagcggat
     9421 gcttcgcacg ttgaccgatg ttgaggattg ccgcaatctg ccagtcagcc actttctgtg
     9481 ctgccgccat ttcgcgcgct ttacctttca cagcctgacg cactgccagc gcgacgcgcg
     9541 cgccggtctg ggtaacgtct tcgcccagca ccagtaccgc atcgtaagat tcgatttcgc
     9601 gcagagccgg agtataaatg ccgccttcac gcagcacttt cagcgccagt tgcagacgtt
     9661 cctgctcacc gtgagcgata ccggtgtaga agttttcttc gcccaccagt tcacgcagcg
     9721 caaagttgct ttccacgctg gcacgcggag aaccaatacc gatcactttc ttcgactgac
     9781 gcagaatatc tgccgcgccc tgcattgcct gttcggcgtt gagggtaatg aaatcatcgc
     9841 cacgacgctg tactggctga cgcggacgat ccttcaggtt gacgtaaccg taaccgaaac
     9901 gaccacggtc gcagaggaag tagtggttta ccgtaccgtt gtaacggttt tcgatacgac
     9961 gcagttcgcc gtaacgttca ccggggctga tgttacagcc gatggaacat tgctggcaga
    10021 tgctcggcgc aaactgcata tcccatttac ggttgtaacg ctcggagtgc gttttgtcgg
    10081 taaatacgcc ggtcgggcaa atttcgacca ggttaccaga aaattcgctt tccagcgtgc
    10141 cgtcttccgg gcgaccgaag tagacgttgt cgtgcgcacc gtaaacgccc agatctgtac
    10201 cgtcagcgta atctttgtag taacgcacac agcggtagca ggcgatgcag cggttcattt
    10261 cgtgagagat gaatggcccc aaatcctgat tacggtgggt acgtttggtg aaacggtagc
    10321 gacggaagct gtgtccggtc atcacagtca tatcctgaag atggcagtta ccgccttctt
    10381 cacataccgg acagtcgtgc gggtggttgg tcattaacca ctcgaccaca ctttcacgga
    10441 actgtttcgc ttcttcgtcg tcaatggaaa taaaggtgcc atcggaagcc ggtgtcatac
    10501 aggacatcac caggcgacca cgcgtgtctt ccgcgttttg gtattgcttc accgcacact
    10561 ggcggcaagc accgacgctt cccagcgccg gatgccagca aaagtaagga atatcaaggc
    10621 ccagagacag acaagcttcc agcaggttgt ccgctccgtt gacctcgtat tctttgccgt
    10681 ctacatgaat tgtagccatt agcatgcttc cagttttctc ggtcagggac cgagcgttaa
    10741 tcgaaattcg gttaccagcg ctctttcagc aggttcggct gaatcccatt aatcaaatgg
    10801 gtattgctga acggctgttt gattcccgcc tcaaattctt cgcggaaata tttgatggcg
    10861 ctctgtaacg gctccactgc accaggtgcg tgggcacaga aagttttacc cgggcctaag
    10921 aatcgacaca gttgctcaag tgtttcgata tcgcccggct ggccttcgcc acgctccagc
    10981 gcacgcagaa ttttcacgct ccacggcaga ccgtcgcggc acggcgtaca ccagccgcag
    11041 gactcacggg cgaaaaactc ttccaggtta cgcaccagcg acaccatgtt gatctcatgg
    11101 tcaaccgcca tcgccagcgc cgtacccaga cggctgcccg ctttaccgat actttcgaat
    11161 tccatcggca gatcaaggtg cgcttcggtc aggaagtcag tccccgcgcc gcctggctgc
    11221 caagctttaa atttcagacc atcacgcata ccaccggcgt aatcttcgag gatctcgcgt
    11281 gcggtggtgc cgaacggcag ttcccacagt cccggatttt tcacccgacc ggagaagccc
    11341 atcagcttgg tgccagcatc tttacttttc gagatgttct gataccactc cacgccgtta
    11401 gcgaggatcg ccggaacgtt acacagggtt tcgacgttgt tgacacaggt cggtttgccc
    11461 catacgccgg aggttgccgg gaaggggggt ttcgagcgtg ggttagcacg acgcccttcc
    11521 agggagttga ttaacgctgt ttcttccccg cagatgtagc gccctgcccc ggtatggacg
    11581 aacagttcga agtcgaaacc tgttcccata atgtttttgc caagcaagcc cgcttcagtg
    11641 gcttcggcaa tggcgcggcg cagatttact gccgcttcga tatattcgcc acgcaggaag
    11701 atgtagccac ggtaagcttt cagcgcaaac gcggagatga gcataccttc caccagcagg
    11761 tgcggcagtt gctccatcaa caggcggtct ttataggtgc ccggctccat ttcatcggcg
    11821 ttacacagca ggtaacggat gttcatggat tcgtctttcg gcatcaggct ccacttcagg
    11881 ccagtcgaaa agcccgctcc gccgcgccct ttcagaccag cgtcttttac ctgattaacg
    11941 atttcgtccg gagacagccc ggtcagcgcc ttacgcgccc cttcgtaacc gtttttgctg
    12001 cggtattcgt ccagccacac tggctgtttg tcatcgcgca gacgccaggt cagcggatgc
    12061 gtttcgggag tacggataat gtttttcatt tataccgctc cagcagttca gggatcgctt
    12121 ccggggtcag atgcgcgtga gtgtcctcat cgatcatcat gtttggccct ttatcacagt
    12181 tccccaggca gcaagttggc agcagcgtaa agcggccatc aaatgtcgtt tgccctggtt
    12241 tgatgttcag ctttttctcg agcgccgcct gaataccctg atacccgttg atatgacaga
    12301 ccacgctgtc gcaataacgg atcacatggc gaccaaccgg ctggcggaag atctgactgt
    12361 agaacgttgc cacaccttcg acgtcgcttg ccggaatacc cagcacatcg gcgatcgcgt
    12421 ggatcgcacc atccggcacc cagccacgct gcttctgaac gattttcagc gcttcaatgg
    12481 acgccgcacg cgggtcttcg tagtggtgca tctcgtgctc aatcgcttca cgctctgccg
    12541 cactcagctc aaaagcctcg gtttgtggtt gttgattctc gtgcataatt agcggtccac
    12601 atctgacata acaaaatcga tactgcccag ataaacaatc aggtcagaca ccaggctgcc
    12661 gcggatcgcc gccggaattt gctgcaaatg cgcataactc ggcgtgcgga tacgagtgcg
    12721 ataactcatg gtgctaccgt cgctggtcag gtagtaactg ttgatcccct tggtcgcctc
    12781 aatcatctgg aaagattcat tggcaggcat caccggcccc cacgacactt gcaggaagtg
    12841 ggtgatcagg gtttcgatat gttgcagcgt gcgctctttc ggcggcggcg tggtcagcgg
    12901 gtgatccgct ttgaacgggc cttccggcat gttgttgagg cactgctcaa gaatgcgcag
    12961 actctggcgc agctcttcca ctttcagcat tacgcgggtg tagcagtcag aaacaccacc
    13021 tccaaccggg atttcaaagt cgaagttttc atagccagaa taaggacgcg ccttacgcac
    13081 gtcgaagtcg atcccggtag cacgcaggcc cgcgccagtg gtgccccact ccagcgcctc
    13141 tttcgcgcca taggcggcaa cgccctggga acgacctttc agaatggtat tttgcagcgc
    13201 cgctttctcg taagacgcca gacgtttcgg catccagtcg aggaactcac gcagcaggcg
    13261 atcccagccg cgcggcaggt cgtgcgctac gccgccaata cggaaccacg ccgggtgcat
    13321 acggaaaccc gtgatcgctt ccaccagatc gtaaattttc tgacgatcgg taaaggcgaa
    13381 gaacacgggc gtcattgcgc cgacgtcctg aataaaggtc gagatgtaca gcaggtggct
    13441 gttgatacgg aacagttcgg agagcataac gcgaatgacg ttaacgcgat ccggcacggt
    13501 gatcccggcc agtttctcta ccgccagcac gtaaggcatt tcgttaacgc agccgccgag
    13561 gtattcaata cggtcggtat acggaatgta gctgtgccag gactggcgct cgcccatttt
    13621 ctccgcacca cggtggtggt agccgatgtc tggtacgcag tcgacaatct cttcgccgtc
    13681 gagttgtaaa acgatacgga aagccccgtg cgccgacggg tggttcggac cgaggttgag
    13741 gaacatgaag tcctcgtttt cggtgccgcg cttcatcccc cactcttccg gtttgaaggt
    13801 cagggcttcc atctccagat cctgtttggc tttggtcagc tcaaacggcg agaattcggt
    13861 agcgcgcgcc gggtaatctt tacgcagcgg gtgacctttc caggtttgcg gcatcatgat
    13921 gcggcgcagg ttcgggtgac cgtcgaaagt aatgccaaac agatcccagg tttcacgctc
    13981 ataccagtta gcgttcggga acagtttggt gaaggtcggt acgtgcaggt cgttttctgc
    14041 cagcgccacc ttcagcatga tgtcgcggtt acgatcgata gaaatcagat ggtagaaaac
    14101 ggaaaaatcc gcggcaggta acccttcgcg gtgtgtgcgc agacgttcgt ccatgccgtg
    14161 taagtcaaac agcatgacgt aaggtttcgg cagtttcttt aagaaatcgc caacttccag
    14221 taattgttca cgcttgatcc acacaacggg aaccccggtg cgagtcgcct gaacagtaaa
    14281 ggcatccggc ccaaaacggt tgcgcagttc gccaatcacc ggatcatcaa gatgatcacg
    14341 ggtctgccaa gcgggttctt gcgcggttaa gtcggtcata ttgttcacca ttgcaaatgg
    14401 tccgtggtga ctgtcggcgc agtaagcttc gcgaaataga tttaggaata agcgaagaaa
    14461 aatccctttg ccgacaggcg caaattaaat ctcgtcaggt gtacgcaggt tggttacggc
    14521 aatgcgttca ccgcgcttgc gttcgcgctc tgattgcata ttggcgcgat aaacgccctg
    14581 atcgccaacc acccaggaga gcggacgacg ttctttgccg atagattcct gcaacagcat
    14641 cagtgcctgc atgtacgctt caggacgcgg cgggcagccc gggatataca catcaaccgg
    14701 gatgaattta tcgacgccct gcacaacgga ataaatatcg tacataccac cagagttggc
    14761 acaggcaccc attgagataa cccattttgg ttccagcatc tggtcataca gacgctgaat
    14821 aaccggtgcc attttggtaa agcaggttcc tgcaaccacc atcaggtcag cctgacgcgg
    14881 cgaagcacgc aatacttctg cgccaaaacg cgccacgtca tgcaccgcgg taaacgaagt
    14941 caccatctca acgtaacagc aggaaagacc gaagttatac ggccaaattg agtttttacg
    15001 accccagtta accatgtcat tgagcttgcc cataaacacg tttttgttaa cttcttgctc
    15061 cagagggtcg gttacgatct cctgcttttg cagggggtaa cggtcgttct caccgttggg
    15121 atctatgcgg gtgagcgtat aatccatctt aatgcctcgc ggttagcgtt gacgattagc
    15181 gatactgttc gtttccgggt tcatacgctc gcggcgtgaa cgcgcgggcg tccagtccag
    15241 cgcgccaata cgcaccagat aaaccagacc tgccagtaac acaaaaataa aaattgcagc
    15301 ttccacaaag cctacccagc cgctttcgcg gatagaggtt gaccatgcga acagatacag
    15361 cgcttcaacg tcgaagataa cgaagaacat ggccaccaga taaaacttgg cggacaggcg
    15421 taagcgggcg gagccgaccg agtcgatacc ggattcaaac ggcacgtttt tcgacctcgc
    15481 gcgtgcgcga ccgcctaaaa accaaccgcc taccagcatc aggcaacaca ggccaatggc
    15541 aacgataaga aagatagcga atgcccagtg atgagcgatg acttcagtgg atgttgacat
    15601 actcattgct tactcatcaa aagtagcgcc agattcactg ctcttcacgg cagatggacg
    15661 ccacatcgat tcatggggag gaataaaaaa aaccttacaa tcactgtaga aattctttta
    15721 tacagctaat tgatgtggtc ttttactcct ttctataacc ttttgtcaac tttaacaaaa
    15781 gtttcttcac attagtttac ataatatcaa caccattagc atttaatgcc ctttcacgcc
    15841 agatccttga cgactccagg ataattagat gttgttgaat cgtgtccgtt gtgaagcaat
    15901 ggaaaaaata cgggtctatt ttgacaggaa tttgtgtcga ttcctccccc caaaagagag
    15961 tattttcttg atctgtgaca cgcttttgtc attccataac aaaaacgcag caacaaattt
    16021 acgcattttt aacatcattg tagcaggtga tttttttcag gcgattattt gtgcgttcgg
    16081 gacgtgaatc tctggtgatt gaaaaatgaa cagttttgta cgttctgcac tatgcgagga
    16141 aggattttta ctaaaaaaaa gccgctgggg tttaaaacac ccccagcggc tcgtttttac
    16201 actattgtct caggaattat ctatcgtccg tcgattactc gatatccctt tcaatcaaca
    16261 atgaatcatc cccttccgga gcagacattg ggctgtattg ccacggatta tggtagctct
    16321 ccatcgcctg atagatcacc tgggccagct cattattact ggacgggtca tagcacagca
    16381 aatattcggt atcaggtagc ggcggtaagc catctactcc actcagcaca cgcaggtacg
    16441 ggctcatcat ttcaatcggc cttgccgtca cgccaagacc ggctttcact gccgcacgaa
    16501 cggccggaag cgtcgaggcg acataagcca gtcgccatgg aatatctgct ttattaagcg
    16561 tcgccagcac catatcgcga aacggacttg gatcatccag caatacaaga gggatcggct
    16621 cgcctttttg caatacgtat tccgctgcgc agtaccagtg tgttggcgag gtacgcaggt
    16681 tcaatgcctt aaacgctgaa ggacgatggg tggttaccat caaatccact tcctgggatt
    16741 ccagcatttc tgccatatag gcattacgtt taacgcggac atccagcgct aatttcggat
    16801 aaaccgaact cacgcgattt aacaggaaag gtaagatcgt atcggcagat tcatctgaag
    16861 caccgatagt taacacgccc tgaagattac tgaacattaa tgatgagcag gcctcatcat
    16921 taaaacgcag tattttcctg gcgtaaccaa gaagttgaat gccatgttca gttaacagtt
    16981 tgttgcgacc gtgacgagcg aacagttctt tcccaacgag ttgttccaga cgctgcattt
    17041 gctgacttac ggcggactga gtacgacaca cagcggcagc tgcggcagca aaagtgttca
    17101 gatcggcaac agcaacaaat gttctcagca gatcgaggtc gaggttaatt atcggacgat
    17161 ttgcacttat catatattat cacttactgg cggctcatac tgagctggtt aatgctgtgc
    17221 acacacaaac aagcaattcc atttgtaatg tgcctccctg gcagtttcat ccggaacccg
    17281 gacgaaagta aaaatgcata tgagttgcac taaaaaagcg actcacattg ttccgttata
    17341 atgcctgaag tagatcacag aatatatctt cagggatcgc atatctatta agttactcac
    17401 tcttttctat ttatgacatg cgcgtgtttg tataaatgta aatgtgagtc cttgttccac
    17461 tctcgtgcag catcgctagt catacgcgaa cacgtacaaa cagcaatggt gaggcggcat
    17521 caagagcagg gatccgtcat ttatgcgagc attttacccc aaaaaccttt tatttataag
    17581 ggtcattgcg aattatctga tgcaaagtta tgttaggcaa agtaatcttc tattattaat
    17641 aagcacatca aaaccttttt gaatattaaa taataattaa ttagcatcat cctcattcat
    17701 taattccgct taacaatagt ttcacacctt gcccccattg cgacacctcg gaaatcatca
    17761 cgctgttaaa ctctccgtaa tgcggaaata tcatcatcgc gtttcattag tgaattctta
    17821 aatgaggcat tttcacacaa ttatcttaca gataaaaaaa ccagacttgc aattaagaat
    17881 cagaacaatt cactatataa cattgcatgc aaagcatata cgcctcatta ttttgtcatt
    17941 attaagttat taacagcaca atcgagcctt cccctctggc aaaatcttat tctgcagacc
    18001 ttcaaaacac cgtcctgggg gagtacattg ttctaagctg acttccacgg cagggagtgg
    18061 cgataacagc aaaaaaggtc aagattcatg tcccccattg aaaaatccag caaattagag
    18121 aatgtctgtt atgacatccg tggtccggtg ctgaaagaag caaaacgcct ggaagaagaa
    18181 ggtaacaagg tactgaaact gaacatcggc aacccagccc cattcggttt tgacgcgcca
    18241 gatgaaatcc tcgttgacgt gatacgcaac ctgcctaccg ctcaagggta ttgcgattcc
    18301 aaagggcttt actccgcgcg taaagccatc atgcagcact accaggctcg tggcatgcgt
    18361 gatgttaccg tggaagatat ttacatcggc aatggtgtat cggagcttat cgttcaggca
    18421 atgcaggcat tgctgaacag cggggacgaa atgttggttc ctgcaccaga ttacccactc
    18481 tggaccgcgg cggtttcgct ttccagcggt aaagcggtgc attatctttg cgatgaatcc
    18541 tctgactggt tcccggacct cgatgatatt cgcgctaaaa ttacgcctcg tacgcgtggg
    18601 atcgttatta tcaacccaaa taacccaacc ggcgcggtat attccaaaga gcttttaatg
    18661 gagattgtgg agattgcacg tcagcataat ctcattatct tcgccgatga aatttatgac
    18721 aaaattctct acgacgatgc tgagcatcac tcaattgcgc cactggcacc tgacctgctg
    18781 accattacct ttaacggact gtcgaaaacg taccgcgttg caggcttccg tcaggggtgg
    18841 atggtgttga acgggccgaa aaaacacgcc aaaggctaca tcgaaggtct ggaaatgctg
    18901 gcttcaatgc gcctgtgtgc taacgttcct gcgcaacacg ccattcagac cgcgctgggt
    18961 ggttatcaga gcatcagtga atttattacc cctggcggtc gtctttatga gcagcgtaac
    19021 cgcgcgtggg aactgatcaa cgatattccg ggcgtttcct gcgtgaaacc tcgtggtgcg
    19081 ctgtatatgt tcccgaaaat cgacgccaaa cgctttaaca ttcacgacga tcagaaaatg
    19141 gtgctggatt tcctgttgca ggaaaaagtt ctgttggtgc aagggacggc attcaactgg
    19201 ccgtggccgg atcacttccg cattgtcacg ctaccgcgtg tcgatgatat cgagctgtct
    19261 ttgagcaagt tcgcgcgttt cctttctggt tatcatcagc tgtaatctta atttcactgc
    19321 cggagattgc atccggcagc gttatcccgc cacaatgacc tgatgatgtc atcatacgta
    19381 aggtcactat gaaacagagc catttttttg cccatctctc ccgcctgaaa ctcattaacc
    19441 gctggccgct aatgcgcaac gtgcggacgg aaaatgtgtc cgaacacagt ttgcaggtag
    19501 cgatggtcgc ccatgcgctg gcagctatca aaaatcgaaa atttggcggt aatgtcaacg
    19561 ccgaacgtat cgctttactg gcgatgtacc acgatgcctc agaagtgctc accggcgatc
    19621 tccctactcc ggtgaaatac ttcaattcgc aaatcgctca ggagtacaag gctattgaaa
    19681 aaatcgctca gcaaaaactg gtcaatatgg ttccggaaga gctgcgggat atctttgcgc
    19741 cgttaattga cgagcatgca tatagcgatg aagaaaaatc gctggtgaaa caggcagatg
    19801 cactgtgtgc atatctgaaa tgtctggaag aactcgcggc cggaaataat gaattcttgc
    19861 tggcaaaaac gcgactggaa gcgacgcttg aagcgcgtcg cagccaggag atggactact
    19921 tcatggaagt atttgttccc agcttccatc tttcgctcga tgagattagc caggattcac
    19981 cgctgtaagc agccggagtc tgcgtcgcat caggcaataa gcgccggatg cgacatcagg
    20041 ctcttgtcaa aacggaaaca gcatcgggat catcaccaca caaaccgcca tcacgataat
    20101 ggtgaacggt acccccaact tcacaaagtc actgaagctg taatttcccg gacctaaaac
    20161 cagtgtgtta acaggtgaag aaaccggtgt cataaaggcg gcggatgctg ccatcgccac
    20221 gaccatcgcg aatggataag gcgacacccc catcgttttg gcagcagcca gcgcaatcgg
    20281 agccatcaac accgccgtcg cggtattaga gataaatagc ccaataaccg ccgacaagac
    20341 aaacaaacac cccagcatca tatgtggccc gtaaccaccg ccaatgtcca tcagcccttt
    20401 caccgccagc gcgacacctc ccgttttctg taatgccaca gcaaacggca tcatcccaac
    20461 gatcaaaata atgctcggcc agtgaatgga tttataggcg ctttcagcat ctatacagcg
    20521 gaatttcccc atcagcaggc aggcgatgat agcggcaaca ggattaggaa tttcatctgt
    20581 cagcattaac gccaccatta gcaccagaca gaaaatggca tggggtgcct ggctgtgcgc
    20641 gggcgatgct tcactcacct cttccggtaa gttcagcgct acgaagtcgc ggccctgttt
    20701 ggccagcata ccgatcagtt tccagttacc cacaaccagg atgatatcgc ccagcagcag
    20761 aggctcatcc gccagcgaac cttccagcgc cacgccattg cgctttagcc ccaccacatt
    20821 cagtccgtag cgggtacgaa aaccaatttc gcgcaccgat ttaccaatca gttctgactc
    20881 aggaattaat gaaatctctg ccatgcccac atcaagggcc tggtcagaaa aatactcgcc
    20941 gcgcagtacc atcggctcca gcaattgctc actacaaaat tgccggagat cgacatcagc
    21001 cgcagacata tcaataagca aaacgtcacg cgcgcgaaat tcagaaaccc cattaacgtt
    21061 cacgataacg cgacgaaaac gccgccagcg ttcaacaccg atgacgttag cgccataacg
    21121 ctcacgtaat ttgagatcat ccagccgttg accaatcatt ggcgatccgg ggcgaatagc
    21181 cagacgtcgc gcacgcccgg tcagtcgata ttcacggata agatcgcgaa aggttcgacg
    21241 cgtccagcct tcgcgctgcg gggtctgggt atcccctttc agcatgaaac gcatcactaa
    21301 catatacaaa atacccagca ccagcacgac caggccaata ggtgttacgc taaagaaact
    21361 gaagccgtga tagccttcac gcagcaattc actgttgact accaggttcg gcggcgtcgc
    21421 caccagcgtc atcatgccgc ttatcagccc ggcaaaactc aacggcatca tcagacgtga
    21481 cggcgaagtt tgcatacgca tggcaacgct taacaccacg ggaataaaga tagcgacaac
    21541 gccggttgaa ctcataaacg cgccaagccc cgcgacggtc agcatcaaca aaaccaacat
    21601 tttgatttca ctgttgcccg caactttgac cagccatgtt cccattacgg tggcaacacc
    21661 ggtacggacc aaaccatcgc caataataaa caaggcggca atcaggacaa cgttaggatc
    21721 agaaaagccg gaaaatactt cggggactgt cagcgttccg cttaatgcaa acgcgacaat
    21781 aacaaacaaa gcgaccgcat ccatacgcac tctgcccgtc gcaaacaaga caatggcaac
    21841 ggccagtaat gaaagaaccc aaatcaattc accgttcaca acttatcctt gttaattgag
    21901 ggagatgtct tgattctgcc ataaaaaagc cccgacgaga cggggctaaa tcatgatcag
    21961 gtgtttcact gaataataac atcgccattt ggctgtttgg tcacagtaat ttgctccaga
    22021 ctgtggagga ccaaatcgac ctcattcagg cgcggggtat ctgccggagc gttaaccgca
    22081 atgacatgac aacccgccgc caggccagaa agcacgccag cggagcatct tccaccacca
    22141 cacactcctg cggcgcaagc cccagcagct gcgcgcctaa cagatacgca tcaggttctg
    22201 gttttccgcg cttcactcgc tcagcggtta caaacacctc tggtgcggga agcccagcta
    22261 ttttatggcg cgctcgcgct accggcatgg agccagaagt cacaatggcc cacggaatac
    22321 ctgctttatt caaatgactg agtaaggcga ttgcccccgg aagcgggtaa taccttcggt
    22381 ttccgtggcc tcgatgtgct ccagacgcgt aaactcggcg gcaatatcag cctcggattt
    22441 gcccgccata aaatggcgca gagaggtgat cgcctgttta ccgtgaatga aagccagcac
    22501 ctcttccggc gctaacccat gacgtctggc ccagttgctc cacgcccgtt ctaccgcagg
    22561 cagggaatcc accagcgttc catcaagatc aaacagaaaa cctttgcacc gcacgcgggc
    22621 ctcctcaggc attgataatt tgattaattt cgttggcgct caaatggtac tgacgcgggc
    22681 aggcatgcca cacattaagc atgcgctgat atttttccca cattggcgtc tgggcgttaa
    22741 aaccgtgagt tccggcgtca aaatgggtat agcgcccttc cacattaacc ataaagcgga
    22801 cataaccgag gtaacgtgct tcagtggcgg cgtcaaagcc gaggaaggtg acacgacgtt
    22861 catcgatgga ttgctggtcc tgcaaattag accaggaaac atgcaacgca tgatacatct
    22921 ccataatgtc gatgatagtg cggcaggttt cttctttcag ctcgccaaac tcgcgatcca
    22981 attcacgcat ctgtaatccg taacctcgct caataattgt ttgcaggcga cggtaacgtt
    23041 cagcatttgc cggatcgagc atagtcatca tcttgtactg attagacaaa ataagacgtt
    23101 gcgcgttggt catttccatt gttgactcct gtatcactct actacggtga aaaaaaagaa
    23161 ggctgagtat gccttctttt atatgcgtaa tcaggggtca attacaaatc atcaaggaaa
    23221 gttttatcca gttgtttgaa ggcgcgctta agcgtgtcag ctaatgcctg gtaatcaggc
    23281 ttgccttcaa cgggtgccaa cacctgtcca gactcctgca atttaccgcg aacttcataa
    23341 aaccagttaa ggattgcagg gggtaatggc gttacagaac gcttgcccag ccaccacaat
    23401 ccctgcatgg gtaaacttaa ggcgaacagt gcagtggcaa ctgccggccc aagctgaccg
    23461 cccagggcaa tctgccagca gagagtaaat acggcgatcg gcggcataaa acggatcgca
    23521 taacgcgtca tcttgataac gcgattttcg acaaagaccg gggcaaggcg tttttccagt
    23581 ggccacgtct ttgagtaatg ctgtccgcgg cgaaacaagc taaaaaaatt aacagaacga
    23641 ttatccggcg ttgacatgct tcacctcaac ttcacatata aagattcaaa aatttgtgca
    23701 aattcacaac tcagcgggac aacgttcaaa acattttgtc ttccataccc actatcaggt
    23761 atcctttagc agcctgaagg cctaagtagt acatattcat tgagtcgtca aattcatata
    23821 cattatgcca ttggctgaaa attacgcaaa atggcataga ctcaagatat ttcttccatc
    23881 atgcaaaaaa aatttgcagt gcatgatgtt aatcataaat gtcggtgtca tcatgcgcta
    23941 cgctctatgg ctccctgacg tttttttagc cacgtatcaa ttataggtac ttccatgtcg
    24001 agtaagttag tactggttct gaactgcggt agttcttcac tgaaatttgc catcatcgat
    24061 gcagtaaatg gtgaagagta cctttctggt ttagccgaat gtttccacct gcctgaagca
    24121 cgtatcaaat ggaaaatgga cggcaataaa caggaagcgg ctttaggtgc aggcgccgct
    24181 cacagcgaag cgctcaactt tatcgttaat actattctgg cacaaaaacc agaactgtct
    24241 gcgcagctga ctgctatcgg tcaccgtatc gtacacggcg gcgaaaagta taccagctcc
    24301 gtagtgatcg atgagtctgt tattcagggt atcaaagatg cagcttcttt tgcaccgctg
    24361 cacaacccgg ctcacctgat cggtatcgaa gaagctctga aatctttccc acagctgaaa
    24421 gacaaaaacg ttgctgtatt tgacaccgcg ttccaccaga ctatgccgga agagtcttac
    24481 ctctacgccc tgccttacaa cctgtacaaa gagcacggca tccgtcgtta cggcgcgcac
    24541 ggcaccagcc acttctatgt aacccaggaa gcggcaaaaa tgctgaacaa accggtagaa
    24601 gaactgaaca tcatcacctg ccacctgggc aacggtggtt ccgtttctgc tatccgcaac
    24661 ggtaaatgcg ttgacacctc tatgggcctg accccgctgg aaggtctggt catgggtacc
    24721 cgttctggtg atatcgatcc ggcgatcatc ttccacctgc acgacaccct gggcatgagc
    24781 gttgacgcaa tcaacaaact gctgaccaaa gagtctggcc tgctgggtct gaccgaagtg
    24841 accagcgact gccgctatgt tgaagacaac tacgcgacga aagaagacgc gaagcgcgca
    24901 atggacgttt actgccaccg cctggcgaaa tacatcggtg cctacactgc gctgatggat
    24961 ggtcgtctgg acgctgttgt attcactggt ggtatcggtg aaaatgccgc gatggttcgt
    25021 gaactgtctc tgggcaaact gggcgtgctg ggctttgaag ttgatcatga acgcaacctg
    25081 gctgcacgtt tcggcaaatc tggtttcatc aacaaagaag gtacccgtcc tgcggtggtt
    25141 atcccaacca acgaagaact ggttatcgcg caagacgcga gccgcctgac tgcctgattt
    25201 cacaccgcca gctcagctgg cggtgctgtt ttgtaacccg ccaaatcggc ggtaacgaaa
    25261 gaggataaac cgtgtcccgt attattatgc tgatccctac cggaaccagc gtcggtctga
    25321 ccagcgtcag ccttggcgtg atccgtgcaa tggaacgcaa aggcgttcgt ctgagcgttt
    25381 tcaaacctat cgctcagccg cgtaccggtg gcgatgcgcc cgatcagact acgactatcg
    25441 tgcgtgcgaa ctcttccacc acgacggccg ctgaaccgct gaaaatgagc tacgttgaag
    25501 gtctgctttc cagcaatcag aaagatgtgc tgatggaaga gatcgtcgca aactaccacg
    25561 ctaacaccaa agacgctgaa gtcgttctgg ttgaaggtct ggtcccgaca cgtaagcacc
    25621 agtttgccca gtctctgaac tacgaaatcg ctaaaacgct gaatgcggaa atcgtcttcg
    25681 ttatgtctca gggcactgac accccggaac agctgaaaga gcgtatcgaa ctgacccgca
    25741 acagcttcgg cggtgccaaa aacaccaaca tcaccggcgt tatcgttaac aaactgaacg
    25801 caccggttga tgaacagggt cgtactcgcc cggatctgtc cgagattttc gacgactctt
    25861 ccaaagctaa agtaaacaat gttgatccgg cgaagctgca agaatccagc cctctgccgg
    25921 ttctcggcgc tgtgccgtgg agctttgacc tgatcgcgac tcgtgcgatc gatatggctc
    25981 gccacctgaa tgcgaccatc atcaacgaag gcgacatcaa tactcgccgc gttaaatccg
    26041 tcactttctg cgcacgcagc attccgcaca tgctggagca cttccgtgcc ggttctctgc
    26101 tggtgacttc cgcagaccgt cctgacgtgc tggtggccgc ttgcctggca gccatgaacg
    26161 gcgtagaaat cggtgccctg ctgctgactg gcggctacga aatggacgcg cgcatttcta
    26221 aactgtgcga acgtgctttc gctaccggcc tgccggtatt tatggtgaac accaacacct
    26281 ggcagacctc tctgagcctg cagagcttca acctggaagt tccggttgac gatcacgaac
    26341 gtatcgagaa agttcaggaa tacgttgcta actacatcaa cgctgactgg atcgaatctc
    26401 tgactgccac ttctgagcgc agccgtcgtc tgtctccgcc tgcgttccgt tatcagctga
    26461 ctgaacttgc gcgcaaagcg ggcaaacgta tcgtactgcc ggaaggtgac gaaccgcgta
    26521 ccgttaaagc agccgctatc tgtgctgaac gtggtatcgc aacttgcgta ctgctgggta
    26581 atccggcaga gatcaaccgt gttgcagcgt ctcagggtgt agaactgggt gcagggattg
    26641 aaatcgttga tccagaagtg gttcgcgaaa gctatgttgg tcgtctggtc gaactgcgta
    26701 agaacaaagg catgaccgaa accgttgccc gcgaacagct ggaagacaac gtggtgctcg
    26761 gtacgctgat gctggaacag gatgaagttg atggtctggt ttccggtgct gttcacacta
    26821 ccgcaaacac catccgtccg ccgctgcagc tgatcaaaac tgcaccgggc agctccctgg
    26881 tatcttccgt gttcttcatg ctgctgccgg aacaggttta cgtttacggt gactgtgcga
    26941 tcaacccgga tccgaccgct gaacagctgg cagaaatcgc gattcagtcc gctgattccg
    27001 ctgcggcctt cggtatcgaa ccgcgcgttg ctatgctctc ctactccacc ggtacttctg
    27061 gtgcaggtag cgacgtagaa aaagttcgcg aagcaactcg tctggcgcag gaaaaacgtc
    27121 ctgacctgat gatcgacggt ccgctgcagt acgacgctgc ggtaatggct gacgttgcga
    27181 aatccaaagc gccgaactct ccggttgcag gtcgcgctac cgtgtttatc ttcccggatc
    27241 tgaacaccgg taacaccacc tacaaagcgg tacagcgttc tgccgacctg atctccatcg
    27301 ggccgatgct gcagggtatg cgcaagccgg ttaacgacct gtcccgtggc gcactggttg
    27361 acgatatcgt ctacaccatc gcgctgactg cgattcagtc tgcacagcag cagtaacctc
    27421 gtcatcatcc gcagctttgc gctgcggata tctgaaccgg aaataatcac tatttccggt
    27481 tttttattct cttaatctgc attaatcctt tctgattatc ttgcttaact gcgctgcatc
    27541 aatgaattgc gccatttcac tttacatact taccactttg ttttgtgcaa gggaatattt
    27601 gcgctatgtc cgcaatcact gaatccaaac caacaagaag atgggcaatg cccgatacgt
    27661 tggtgattat cttttttgtt gctattttaa ccagccttgc cacctgggta gttccggtgg
    27721 ggatgtttga cagtcaggaa gtgcagtatc aggttgatgg tcaaacaaaa acacgcaaag
    27781 tcgtagatcc acactcattt cgcattctga ctaacgaagc aggcgaacct gagtatcacc
    27841 gcgtacagct gttcacgacg ggcgatgaac gcccgggcct gatgaacttc ccgtttgaag
    27901 gattaacctc aggatcgaaa tacgggacag ctgttggcat catcatgttt atgctggtga
    27961 ttggcggcgc gtttggcatt gtgatgcgta caggaaccat tgataacggt atcctggcgc
    28021 ttattcgcca tacccgcggg aatgaaattc tctttattcc tgcgctgttt attctgtttt
    28081 cacttggcgg cgcggtattt ggtatgggag aagaggccgt cgcctttgcc attatcatcg
    28141 caccgctaat ggtccggctg ggctatgaca gtattaccac cgtcctggtg acctatattg
    28201 ccacgcaaat cggttttgcc agttcgtgga tgaacccgtt ttgtgtggtc gttgctcagg
    28261 ggattgccgg cgttccggtg ctttctggct ccgggttgcg catcgtggtg tgggttatcg
    28321 ccactatgat tggcctgatc tttaccatgg tgtacgcctc acgagtgaaa aagaatcctc
    28381 ttctgtcacg cgtgcatgag tccgaccgct tctttcgtga aaagcaggcg gatgttgaac
    28441 aacgtccgtt tacctttggt gactggctgg tattgattgt cctgaccgcc gtaatggtct
    28501 gggtgatttg gggcgtgatc gttaatgcct ggtttattcc agaaattgcc agccagttct
    28561 tcaccatggg tctggtgatt ggcatcatcg gcgttgtttt ccgccttaac ggcatgacgg
    28621 ttaataccat ggcttcatcc tttaccgaag ggacgcgaat gatgatcgcc cctgccctgc
    28681 tggtgggttt cgccaaaggg attttgctgc tggtcggtaa tggtgaagcg ggtgatgcca
    28741 gcgtgttaaa taccatcctc aacagcattg ccaatgccat tagcggtctg gacaacgcgg
    28801 tcgcggcctg gtttatgttg ctcttccagg cggtatttaa tttcttcgtg acgtccggtt
    28861 ctggtcaggc ggcgttaacc atgccgttac tggcaccgct tggcgatctg gtcggtgtta
    28921 accgtcaggt taccgtgctg gcattccagt ttggtgatgg cttcagtcac atcatttacc
    28981 caacctcagc ttcgttaatg gcaactctcg gtgtgtgcag ggtggacttc cgtaactggc
    29041 tgaaggtggg cgcgacccta cttggactgc tgtttattat gtccagcgtc gtagtgatcg
    29101 gcgctcagtt gatgggctac cactaaaaat gttaagagcc gcaattgcgg ctcttttttc
    29161 attctgccgt ttcagtctct acggcttcat ttttggcatt gcgcttcatc cacaacgcca
    29221 gcgctttcag cgagtctgga gtgaactcat cgcagcgtgc ggtgatttct tccggcgtca
    29281 gccagcaaac ttcactgact tcatcttcct gtagggcgaa gggaccgtga gagacgcagc
    29341 tgaacaacgc gccccagaca cggcaatttt tatcttcgaa atagaactgc ccgtgctcgg
    29401 caaaggggac accggcaatg cccaactctt cttccgcttc gcgacgcgcg gattccagca
    29461 gttgctcatc ggcctgaacc acaccgcctg cggtcgcatc taacatgccg ggtaaaaagt
    29521 cttttgtctc ggtacgacgc tggaccagaa ttttgcccat gccatcatgc acgacgatgt
    29581 aagttgcacg atgacgcaga cactgtgccc gcatttgttc ccggctggct tgtgcaatga
    29641 cttcgttctc ttcattgaca atatccaccc attcagtact tgccaaacga cgctgttcca
    29701 ttatcaggaa accttctttt tctggcgctc ttacggcgca ttttgagttg tgggtaaatt
    29761 acggattaat cgcgacctgc gcaatgatac tttgatcatt gagtgcgatt acactaagta
    29821 cgtcattatc cagcatgcca taactcgccg gattaccgcc tttcggaata ctcaccgagc
    29881 cggggttgaa gtggaaaatc tccccccgct gttctgccac tggcagatgg gtatgaccgt
    29941 acaccagcac atcgttctgg tttaaagcag gtagattttc cgggccaaaa agatgaccat
    30001 gcgtcaaaaa cagacgttgt ttttccagta atacctgttg ccacggcgcg gttatcggga
    30061 aatgcagcag catttgatcc acttcgctgt cgcagttgcc gcgcacagcg ataaccttat
    30121 gtgccacttc attaagccgt tcagcgactt tggctggcgc gtaaccctcc ggtaaagcat
    30181 tacgcgggcc gtgattcaac acgtcgccaa ggatcaccag ccactgggca ccgctttggg
    30241 caaacaactc cagaacacgt tccgtcgccg gtaacgaccc atgaatgtcc gatgcaaaca
    30301 tcagtttcat cactcactcc tcgtcgaaaa aacgtaagct atgatactgg attctgtcgt
    30361 tgctatcagc cagattgctt cgccgaaagt gcaataaaac gctggacaga cgctcgctgc
    30421 cactggaatg tcgcataatc caccaggcgt tctggcacct catcgccatg tagtaccagg
    30481 cggttaatca ttagcgccag atcagtatca gcaatgcacc attcaccaaa taaattcggc
    30541 tgacccagtg ctaacaaatg ttctgccatc gcgaacagtt tctctgcact ggcttttccc
    30601 tcggccgtta gtggcgcttt tttcgccccc gcaaagacaa catccgtcgg acgctcttcg
    30661 cggatgggca tcagatcgct gcgcagccag gcctgaatcc gtcgtgcacg cgcacgattt
    30721 tctaaatcaa gcggataaat acgttcccag gtcggtggcg caaatcgatc ttccagatac
    30781 tccgcaatgg cagaagattc actcaactca aaatcatcga tttgtaataa cggcacacgg
    30841 cgtgtctgac cgtaaccttg ccacgtcggc ttcaaatgtt caccgctgtc gaggtcgatg
    30901 gtcttgatat gaaacgacag gcctttttcc tgcaacgcca cccaggcgga taacacataa
    30961 ggggagaaaa agtgggcatc tgaccaaagc gtgatagcgg gtttactcat tatgtcctcg
    31021 gcaataaagg gggctggcta tcaaactata gagccttttt gtcactgtca cgtgacgaat
    31081 actcacgtaa gtcgccgcgc tttctatact tgtggtgttc agatgattaa acaaccggag
    31141 ctgcaatgat cgatctctat ttcgccccga cacccaatgg tcacaaaatt acgctgtttc
    31201 tcgaagaagc agagcttgat tatcgcttga ttaaggtaga cctggggaaa ggcggtcagt
    31261 ttcgtccgga atttttgcgc atttcgccca acaacaaaat tccggcaatt gttgatcatt
    31321 ctcctgccga tggcggcgaa ccgctaagcc tctttgagtc tggtgccatt ttgttgtatc
    31381 tgactgagaa aacaggactc tttttgagtc atgaaacgcg tgagcgcgcc gccacattac
    31441 agtggttatt ctggcaggta ggcggactgg ggccgatgct tgggcaaaat catcatttta
    31501 atcacgcagc cccccaaacc attccttacg ctattgaacg ttatcaggtt gaaactcagc
    31561 gtctttacca tgtactaaac aagcggctgg aaaactcgcc ctggctggga ggcgagaact
    31621 acagcattgc ggatattgcc tgctggccgt gggttaatgc ctggactcgc cagcgaattg
    31681 acctcgcaat gtatccggca gtcaagaact ggcatgagcg gatccgttcg cgccctgcca
    31741 ccgggcaggc actgctaaaa gcacaactcg gtgatgagcg ttcggatagt taacagaaac
    31801 aggttctcgt gtattatttc atcctaagta aaacaacgga gaacctgcaa tggcacaacc
    31861 tgccgctatt attcgtataa agaaccttcg tttacgtacg tttatcggaa ttaaggaaga
    31921 agaaattaac aaccgtcagg atattgttat caatgtgacg atccactacc ccgccgataa
    31981 agcgcgcact agcgaagata tcaacgatgc gctgaattat cgcaccgtaa cgaaaaacat
    32041 tattcagcat gtagagaata accgtttctc tttgttggaa aaattaactc aggatgtgct
    32101 cgatatcgca cgtgaacatc actgggtgac gtatgctgaa gtggagatcg ataaactgca
    32161 cgcgctgcgc tacgccgatt cggtatccat gaccttaagc tggcagcgtt aaccgccata
    32221 tcgggaggct gcatgaacat agtgatcacc ggagggacgg gattaattgg tcgccatttg
    32281 attccgcgtt tgctggagct gggccatcaa atcacggtag tgacgcgtaa cccacagaaa
    32341 gccagttccg ttctcggccc tcgggtgacg ctatggcaag ggctagccga tcaaagcaac
    32401 ctcaacggcg ttgatgcggt aatcaacctg gccggagaac cgattgctga taaacgctgg
    32461 actcacgagc aaaaagagcg tctctgccaa agccgctgga atatcacgca aaaactggtc
    32521 gatttgatta atgccagcga cacgccgccg tcggtattca tttccggctc ggcaacgggc
    32581 tattatggcg acttaggtga agtggtggtt accgaagagg aaccgccgca taacgaattt
    32641 actcataaac tctgcgcccg ctgggaagag attgcctgcc gggcgcaaag tgacaaaacg
    32701 cgagtgtgcc tgctgcgaac cggcgtggta ctggcaccgg acggcggtat tctcggtaaa
    32761 atgctgccac cgtttcgtct tggcctgggc gggccgattg gttccggtcg gcagtatctg
    32821 gcctggattc atattgatga tatggtcaac ggcattctct ggctgctgga taacgacctg
    32881 cgcgggccat ttaatatggt ttcgccctac ccggtacgca atgaacaatt tgcccatgcg
    32941 ctcggtcaag cgctgcatcg cccggccatt ttgcgcgtcc ccgcaaccgc cattcggctg
    33001 ttaatgggcg aatcttcaat actggtatta ggtggacaac gcgcgctgcc taaacggctg
    33061 gaagaagcgg gttttgcgtt tcgctggtac gatttagaag aggcgctggc ggatgtcgtt
    33121 cgctgatgtg gtttacagca aacatccgcc agttaactcc cggtgttaca ggattagtgg
    33181 ctttgcgcga taagatcgtc tggtgaaagt cgggtcacca tcataactaa ctctctgtct
    33241 aaacctctat ccagcatctc ctgagcaata cgcagggctt cttcgtgttt gccctgcatt
    33301 gcgccttctt cacgtaatct gtcagcaatg gtcatcagtt tctccttttc ttgtggtgcg
    33361 cgttctgtta tctcaccaat aaatgcacgg aaacgctggg catcccctgt ttgtaatacg
    33421 taattaaaca gggcttttag ctgtctgtca ttagtgttcc ctgtaactag cagcgaaaca
    33481 atttggtcga ctaatcccaa cagatcgcgc tgacgaatat gtttctgaat taactccaac
    33541 agcgccattt tgcggtgttg cataatctcg tcatccggca ccacggtaat atccaccaac
    33601 ggaaacgctg atgaatatat tttgcgggct atagcaggct cggcaaattc atcaagccag
    33661 cagagtgaat aaggataagg acttctgcaa ccatgataaa acagcatcgg gatcaccaat
    33721 ggaagctctt tatagcccgc atcaagatgg ttttgcattg ccgcaatgga ataacgcatc
    33781 atgcgaaaag ccattaattc ttccagctta ctttggtgct ctatcactac ataaatataa
    33841 cccactccct cctgcgtttt cacagaccac aagaggtcgg aataatattg ccgcaggtct
    33901 tcatcaataa aactgtttga ttctagttta agcgtcgtta aatcacacag tttgcgcagc
    33961 ggcgcgggaa gatgaatatc aataaaatcc cgcgcggtgt
//
LOCUS       DAJVRF010000030        33995 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJVRF010000030 DAJVRF010000000
VERSION     DAJVRF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.030"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(1..2601)
                     /gene="acrF"
                     /locus_tag="OQN13_001319"
                     /pseudo
     CDS             complement(1..2601)
                     /gene="acrF"
                     /locus_tag="OQN13_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(2613..3770)
                     /gene="acrE"
                     /locus_tag="OQN13_001320"
     CDS             complement(2613..3770)
                     /gene="acrE"
                     /locus_tag="OQN13_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCS1614719.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            4169..4831
                     /gene="acrS"
                     /locus_tag="OQN13_001321"
     CDS             4169..4831
                     /gene="acrS"
                     /locus_tag="OQN13_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCS1614720.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(4834..5013)
                     /locus_tag="OQN13_001322"
     CDS             complement(4834..5013)
                     /locus_tag="OQN13_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCS1614721.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(5097..5981)
                     /gene="yhdJ"
                     /locus_tag="OQN13_001323"
     CDS             complement(5097..5981)
                     /gene="yhdJ"
                     /locus_tag="OQN13_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS1614722.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(6067..6363)
                     /gene="fis"
                     /locus_tag="OQN13_001324"
     CDS             complement(6067..6363)
                     /gene="fis"
                     /locus_tag="OQN13_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCS1614723.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(6389..7354)
                     /gene="dusB"
                     /locus_tag="OQN13_001325"
     CDS             complement(6389..7354)
                     /gene="dusB"
                     /locus_tag="OQN13_001325"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCS1614724.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(7683..8564)
                     /gene="prmA"
                     /locus_tag="OQN13_001326"
     CDS             complement(7683..8564)
                     /gene="prmA"
                     /locus_tag="OQN13_001326"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCS1614725.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(8576..10027)
                     /gene="panF"
                     /locus_tag="OQN13_001327"
     CDS             complement(8576..10027)
                     /gene="panF"
                     /locus_tag="OQN13_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCS1614726.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(10017..10259)
                     /locus_tag="OQN13_001328"
     CDS             complement(10017..10259)
                     /locus_tag="OQN13_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCS1614727.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(10368..11717)
                     /gene="accC"
                     /locus_tag="OQN13_001329"
     CDS             complement(10368..11717)
                     /gene="accC"
                     /locus_tag="OQN13_001329"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCS1614728.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     RGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(11728..12198)
                     /gene="accB"
                     /locus_tag="OQN13_001330"
                     /gene_synonym="fabE"
     CDS             complement(11728..12198)
                     /gene="accB"
                     /locus_tag="OQN13_001330"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCS1614729.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(13176..14150)
                     /locus_tag="OQN13_001331"
     CDS             complement(13176..14150)
                     /locus_tag="OQN13_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1614730.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            14302..16242
                     /gene="csrD"
                     /locus_tag="OQN13_001332"
     CDS             14302..16242
                     /gene="csrD"
                     /locus_tag="OQN13_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCS1614731.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAAQLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            16547..17590
                     /gene="mreB"
                     /locus_tag="OQN13_001333"
     CDS             16547..17590
                     /gene="mreB"
                     /locus_tag="OQN13_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCS1614732.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            17656..18759
                     /gene="mreC"
                     /locus_tag="OQN13_001334"
     CDS             17656..18759
                     /gene="mreC"
                     /locus_tag="OQN13_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCS1614733.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            18759..19247
                     /gene="mreD"
                     /locus_tag="OQN13_001335"
     CDS             18759..19247
                     /gene="mreD"
                     /locus_tag="OQN13_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCS1614734.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            19256..19849
                     /gene="yhdE"
                     /locus_tag="OQN13_001336"
     CDS             19256..19849
                     /gene="yhdE"
                     /locus_tag="OQN13_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCS1614735.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            19839..21308
                     /gene="rng"
                     /locus_tag="OQN13_001337"
     CDS             19839..21308
                     /gene="rng"
                     /locus_tag="OQN13_001337"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCS1614736.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            21376..25176
                     /gene="yhdP"
                     /locus_tag="OQN13_001338"
     CDS             21376..25176
                     /gene="yhdP"
                     /locus_tag="OQN13_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCS1614737.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            25332..26777
                     /gene="tldD"
                     /locus_tag="OQN13_001339"
     CDS             25332..26777
                     /gene="tldD"
                     /locus_tag="OQN13_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCS1614738.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(26905..27770)
                     /locus_tag="OQN13_001340"
                     /pseudo
     CDS             complement(26905..27770)
                     /locus_tag="OQN13_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
     gene            27953..28156
                     /locus_tag="OQN13_001341"
     CDS             27953..28156
                     /locus_tag="OQN13_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCS1614739.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            28164..29096
                     /gene="aaeA"
                     /locus_tag="OQN13_001342"
     CDS             28164..29096
                     /gene="aaeA"
                     /locus_tag="OQN13_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCS1614740.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            29102..31069
                     /gene="aaeB"
                     /locus_tag="OQN13_001343"
     CDS             29102..31069
                     /gene="aaeB"
                     /locus_tag="OQN13_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCS1614741.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            31161..31433
                     /locus_tag="OQN13_001344"
     CDS             31161..31433
                     /locus_tag="OQN13_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCS1614742.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(31489..31752)
                     /gene="yhcN"
                     /locus_tag="OQN13_001345"
     CDS             complement(31489..31752)
                     /gene="yhcN"
                     /locus_tag="OQN13_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCS1614743.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(32117..32587)
                     /gene="argR"
                     /locus_tag="OQN13_001346"
     CDS             complement(32117..32587)
                     /gene="argR"
                     /locus_tag="OQN13_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCS1614744.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            33022..33960
                     /gene="mdh"
                     /locus_tag="OQN13_001347"
     CDS             33022..33960
                     /gene="mdh"
                     /locus_tag="OQN13_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS1614745.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT         8255 a         8454 c         8900 g         8386 t
ORIGIN      
        1 tcctgatacg acatacctgt ccagtcataa ccaatgcccg caggtaattt tgacgcaagg
       61 ttttccatca atgccatggc atcgccggaa ctggttcctg gcgcggcttc cccctgaatc
      121 tccattgacg gcagaccgtt gtagcgttcc agtcgcggag agccatacac ccaatgtgaa
      181 gtggtaaagg cagagaatgg caccatttcg ccgttggcgc tgcggacata aagtttatcg
      241 acatcttctg gcagcatacg gaatttggca tccgcctgaa catacacctt tttcacgcga
      301 ccacggtcga tgaagtcgtt aacgtaagtc ccacccagcg ccgttgaaat ggtctgattg
      361 atgtcagaaa gtgaaacacc taatgcctgc gccttttcct ggtcaacttc cagtttaaac
      421 tgcgcggtgt cttccagacc attaggacgc acgctgacta agctggcagg atgttgcgcc
      481 gccatgccaa gcaactggtt acgggcctgg gtcagggcat cgtgacccag cccagcctga
      541 tcaattaact caaagtcgaa acccgttgcc gtgcccagtt caacaatggc tggcatattg
      601 aatggaatga caaaaccgtc gcggatcttg cccaattcca ttttggcacg atggattacc
      661 gcttccgcac tgttttcgtc accactacgc tcttcccacg gtttcagact gacgaaagcc
      721 ataccggcgt tttgtgcctg gccgctgaag ctaaagccgt taaccgtaaa gacactttct
      781 acgttcgctt tctcgttatt cagatagtaa tccgtcactt gatccaacac tttttgcgtc
      841 cgctcttgcg tcgcgccagc gggtaactga atcatggtca gaaagacacc ctgatcctct
      901 tcaggtaaga aggaagaagg aagacggaag acgtaaaaac aacaccacca ttcctgcaac
      961 aatcagcgca tagatcagta aatatcgtcc tgtggaaccg aggattttgc cgacgctgtt
     1021 ggtgtagtgg ttaacgctat gatcgaaggt ggtattaaac cagccgaaga aaccgccctt
     1081 attttcgtga tgctcagcag agacgggttt aagcagcgtt gcacataacg caggggtaag
     1141 aatcaatgcc accagaacag aaagcgccat tgccgaaacg atggtgatag agaactggcg
     1201 ataaattgcc ccagtagaac cgccgaagaa tgccatcgga ataaataccg ctgacagcac
     1261 catcgcgata cccaccagtg cgccctgaat ttgcgacatc gatttttccg tcgcttcttt
     1321 tggcgggagc ttatcctcca tcatcacgcg ctcgacgttc tccaccacca ctatcgcatc
     1381 atcgacgagc agcccgatgg caagcaccat cccgaacatc gttagtgtgt tgatggagta
     1441 accaaaagcg gcgaggatgg caaacgtccc taacaacacc acgggtaccg caatggtggg
     1501 gatcagcgtt gctcgcatat tctgcaagaa cagatacatc accaggaaca ccagcataat
     1561 ggcttcgaac agcgttttta ccacttcgtg aatagaaagc tggacgaatg gcgtggtgtc
     1621 ataagggtag agaaccttca ttccctgcgg gaagaatggc tgtaattccg ccagttttgc
     1681 cttaatggct ttcgcggtat tgagagcgtt cgcgccggtt gctagcttaa tccccaggcc
     1741 cgccgccggt tttccgttga tacgagcgat aacgttatag ttttcaccgc caagttcaac
     1801 tcgtgcgaca tctttcaggc gtaccaccga gccgtcactg tttacgcgca gggttacttt
     1861 gccgaattct tccggatttt ttaaccgcgt ctgagcaata atcgaggcgt tcaactgttg
     1921 ccctggtaac gctggcgttc cgcccaactg cccggcagcg atctgatcgt tctgtacctt
     1981 caactggtta atcacatcaa ccggtgtcag tttatatttg tttagcagat cggcgtccag
     2041 ccagatacgc atcgcatact gtgcgccgaa aagctgtacg tcaccgacgc cattcagacg
     2101 gctaagcgta tctttaacgt tagaggccac atagtccgag atatcgtcct gtgtggtgtc
     2161 tgggttatca gagacaaagc ccgccaccat caaatagctg ctactggact tttcaacact
     2221 gatcccctgc tgctgaacct cctgcggcag caacggcgtg gcgagctgga gtttgttctg
     2281 cacctgcact tgcgcgatat caggatcggt cccggactgg aaggtaaggg taattgtcac
     2341 gctaccggcg gaatcgctgg tggaggacat atacatcagg ttatcgatac cattcatatt
     2401 ctgttcgata acctgcgtca ccgtatcctg cacggtctgc gcatcagcac ctggatagtt
     2461 tgcctaaaca gaaaccgcag gcggtgcaat ggttggatac tgagcgacgg gcaattgtag
     2521 gatcgccagt gcgcccgcca tcatcagaat aatggccagc acccatgcaa atatcggtcg
     2581 tcgaataaaa aagtttgcca tgtcagatta ccttacttcg atgcagtatc tgcgggggta
     2641 tcggtagtgg ctttaacctg ctctcccgga cgcgcttttt gcaggccgct gacaatgact
     2701 tgatcgccag atttcagacc ttcgctaatc aaccatttat cgccaatcgc ctgactggca
     2761 acgacagggc gcgcttccac ctgactttta tcgttaacaa tcagcacggt tgcatcacca
     2821 cgcggtgtgc ggctaacgcc ttgttgcggg ataagaatgg cgtcaggttg gacgccttca
     2881 tcaatccgtg cacgcacaaa catacccggc aaaagcgtat gttgcgggtt agggaagaca
     2941 gcacgtaggg ttatggagcc ggtgcattca tcaacggtca catcagagaa ttgcagcgta
     3001 cctttcaggg gataggtttg accgttttcc atgaccaact ctacgttgct ggtggcgttt
     3061 tccttatgca aatttccttg ctctacggat tgcttcagcc tcataaaatc gttgctggat
     3121 tgggtcacat caacgtagat aggatcgagc tgctggacag tcgccagttc agtcgtttgc
     3181 ccattagtga caagagcgcc ttcggtcacg gtcgatttgc cgatacgtcc gctaattggc
     3241 gcagtcactt tggtataagc gagattgatg cgagcgcttt cgactgtggc ttttgcggca
     3301 atcacggcgg catcggcctg gcgagcatca gcaatggcct ggtcgtactc ctgctggctg
     3361 atgtatttcg tacccacgag cggaacgtaa cgttttaccg tcaaatgcgc gatggcggcg
     3421 gcggcttcac ttttcgccag ttcgcctttc gcgctgtcat aatttgcctg ataggtcgcg
     3481 ggatcgatct ggtacaggga ctggcctgct tgtacatcgc tgccttcagt gaaattacga
     3541 ttcagtacga tcccgctaac ctgtgggcga acttcggcta tacggtaagc attggtgcgg
     3601 cctggtaatt cagtcttaac ttctaacggg gccgttttca caatatgaac ggtaacctgc
     3661 ggttcaccga cgtgagcttt ctcttctccc ttatcgttac aaccgacgat taaagccgcg
     3721 gagatcagaa taaaggaggg caggaggaaa aacctggcat gtttcgtcat tactattcct
     3781 caaaaaaaca aaagcgcgtt atttacccag aaaggcaacg cgttaactcg cagaaagaaa
     3841 aatacagttt gctatcctac aaattatcat tcgtcgatgt aaggaatagt tatgaatact
     3901 ggcgtctcaa ggcacataaa cacaaaaaaa gattaatatt ctactgtttt attttgacgc
     3961 gggttgaaag aggcagaatt aaaacctcgt aaattgaaat atatattcat gtggcgaatg
     4021 tattttaggt aaataatata tattatttat ttgacgattt atagcaactc aatgttaccc
     4081 tctcgtataa atacacatta gatgatagat taaccttcgc tattttctca ccctgtgtcg
     4141 aatatattta tttcctgaat aattaatcat ggcaaaaaga accaaagccg aagctctgaa
     4201 gacccggcaa gaactgattg aaactgccat cgcccagttt gcgcaacatg gcgtaagcaa
     4261 gacgacgttc aacgacattg ccgatgccgc taacgttacg cgtggcgcta tctactggca
     4321 cttcgagaac aagacccaac tgtttaatga gatgtggttg caacagcctt cattgcggga
     4381 gttaatccag gaacacttga cggctggatt agagcatgac ccgtttcaac aattgcgtga
     4441 aaaattgatt gtcggcttgc aatatattgc caaaattccc cgccagcagg cgttgctgaa
     4501 aatcttatat cacaaatgtg aatttaatga tgggatgctg gccgagggag tgatacgcga
     4561 aaagatgggc tttaatccgc agactctccg cgaagtattg caggcgtgtc agcaacaagg
     4621 ttgtgtagca aataacctcg atttagatgt tgtgatgatt attattgatg gtgccttcag
     4681 cggaattgtt caaaactggt taatgaatat ggcgggttat gatctttata aacaagcacc
     4741 cgcgctggtc gataacgtat taagaatgtt catgccagat gaaaacataa cgaaattaat
     4801 tcatcaaaca aatgaattaa gtgtcatgta aattcacatt ctgtcctgaa aattcagttc
     4861 gtcacagtta accagtttta gtggataaac tgagttcata ttacggcatt tgtctgtttc
     4921 tgttgtcagc agttcaatcc actgcatcag taaagtatca aacagaaaaa cgaagacagc
     4981 gaaaacgacg agccaccaat acttgcgaat cattacctga gcctgtgcat gtttaaccaa
     5041 gtctggcagg aaatatacac gaaatgaata atctgaaaac caggcttaca cttcctttac
     5101 tttgcaatga gatcggggtc aacttcgctt aaccggcttc gttttgacag gttgccggtc
     5161 tttctttttt tcactttcgc cagttcttcc gcggagtaat gcgacgcgac atccagccgt
     5221 cgaagcccca ttttgatgta ctcgctgttg atctcaatac caatgaattt tcgtccgctg
     5281 gcgatggcta cggcaccggt agtaaagcta ccggcaaacg ggtcgagaac gatatcgcct
     5341 gggttggaag aggcgagaat aatgcgtttc agtaaggctt ccggtttttg cgtcgggtgg
     5401 ttctcatatt catccatcaa ataacgcacg cgcggaaaat cccagacgtt acccggtact
     5461 ttttgatgat tgtatggctg tggaggattt ttgcgataat cgatcaacgc gcgctgcgat
     5521 ccggttttgg cttctaccag aatagcatca ccgttgaatg tgtagttctt tgcgtctttc
     5581 accatcatca ggatgggttc gtacatggag ccgtagtgtt ttttcgcctg tactccagaa
     5641 ctgtcatatg accagacgat gcgacttttg atggtaaaaa gcttgcggca ctggagatcg
     5701 ataaagggca tgttttccgt actgttcata atgtacatgc tgccctgctt tttcagaacg
     5761 cggtggcact ctacaatcac ttcaaacagc cagtcgataa acagatcttc tttccaggct
     5821 tcgatcagac catcaaaatt tttaccgatg ttatacggtg ggtcggcaaa gatcagatcg
     5881 acgctttcag taggtagctt tttaagttcg gcaagggcat caccgtgaat aatggtctta
     5941 gcttcattac caaaccgggt cggttcacat cctgttctca tggtcactcc ctttgtgaca
     6001 cctataaaaa aggcgcttcc ccatgccgag tagcgccttt ttaatcaagc atttagctaa
     6061 cctgaattag ttcatgccgt attttttcaa ttttttacgc agcgtaccac ggttgatgcc
     6121 catcatcagc gcagcacggg tctggttacc acgggtgtat tgcatcacca tgtccaacag
     6181 gggctgttct acttcagcca gtaccagctc atagaggtca ttcacatcct gaccattcag
     6241 ttgagcaaaa tagttcttca gtgcctgttt aaccgagtca cgcaggggtt tttgggttac
     6301 ctgatcctga gagttaacgg tagaaacggt cagtacgtca gaatttacgc gttgttcgaa
     6361 catagttctg tcagctcttt atttctgttt acgcaaaatt ttcgaagtat gcctccaacg
     6421 cctccagctg ttcgctggca tcctcaatgg cgttgaatgt gcgccgaaac tggtcatttg
     6481 gagcgtgttc ctggagatac caggaaacgt gtttacgtgc aattcggtac ccttttgccg
     6541 gaccataaaa gtcatgcagt tcccgaacgt gcgcgcaaag caagcgctta acctctgcca
     6601 aaggcagcgg gggcagcaac tccccagtgt ccagataatg ctggatttcc cgaaagatcc
     6661 agggtcttcc ctgagctgcg cggcctatca tcagggcatc cgcccctgta tagtcgagca
     6721 cagctctggc tttaagcggg tcagtaatgt cgccattcgc gataaccgga atggaaactt
     6781 tctgcttaac tgcccgaata ctgtcgtact cagcttctcc attgaacaaa caggcgcgtg
     6841 tacggccatg aatggtcaga gcctgaatgc cacagtcttc agccagttgg gcaatctctt
     6901 cgcagttacg gtgttccggt gcccagccgg tgcgaatctt cagggtaaca ggaacgtcca
     6961 ctgcattgac gacctcggta aggatcgatt taacgacatc cgggtactgc aagagggctg
     7021 aacctgcgag cttgcgattc acttttttag ccgggcaacc catattgata tcaataatct
     7081 gggcaccgct ttccacgtta atacgtgctg catctgccat ttctttcgga tcgctaccag
     7141 caatttgcac ggtgcgaata ccgggttcat caatgtgcac catccgtaaa cgagatttgt
     7201 cgctttccca aacctgtggg ttagaagaca tcatctcgga tactgtcaat ccggctccca
     7261 tctcgtagca caacgtccga aaaggtctgt ctgtaatgcc agccatgggc gctgcgatca
     7321 ggcgatttct gagctgatat tgtccgatgc gcatgagtta agaaatgacc atactgtgac
     7381 tgcaaggcgg cgtatattac gcattttttg cacgagatga aaggccaaac tttgaccaat
     7441 cctctgagat ggatcaaaga attgcattta aaatgagcgt ggtgagataa ttactcataa
     7501 aaatcatcat attagaaaat agtgactaaa atttacactc aaagaaattt gagtaagttc
     7561 tcaatttttc tttatgaatg aaaattttgg cacgcaaatt tgcgtaaata accggcaaat
     7621 ttacgtgcct tttgtgagct tgctcgcact tcgccccgcg tcaccctacg gcgatgcgaa
     7681 ggttaattct tacgaccggt aatgcggcac cactcttctt tttccacgac cgggtccagt
     7741 gcgaagctgt cgacataagc ttcacaaacg ctctctgcct ggcttgccag aataccggaa
     7801 aggcccagca aaccgcctga aaccggcagg acgctgatta acggtgccag ttcacgtaat
     7861 gggcctgcaa ggatgttagc gaccaccacg tcggctttca tttcttctgg ctgatctttc
     7921 ggtaagtaga gttccagacg gtcagaaacg ccattacgtt cggcgttgtc gcggctagcc
     7981 tgaatcgcct gcggatcgat atcaatacca atggcttttg ctgcacccag tttcagcgcc
     8041 gcgatagcca gaatgccgga accacagcca aagtcgatga ctattttacc ggttaaatcg
     8101 aggctgtcga gccactgcag gcacagagag gtagttggat gggtacccgt accaaacgcc
     8161 agccctggat ctaacatcac gttgacggcg ttttcgtccg gcacatcacg ccagctaggg
     8221 cagatccaca gtcgttcacc aaagcgcatc gggtggaaat tatccatcca ttcgcgctcc
     8281 cagtctttat cttccagttg ttcgatttta tgcgcgaagc ctgcgccgag cagcggatgg
     8341 ttttccagaa tcgccaccac gtcgttcata tcggtttcag cgtcgaacag accaatcaca
     8401 tcggtgtcgc cccacaggcg cgtttcgccc ggcagcggct caaatactgg cgtatcgtgg
     8461 gtatcctgaa aagtgataga aacggcaccc gcttccatca gcgcatcgct aagatcttca
     8521 gcgttcgcgc cggtggtgtt cagtttcagt tggatccaag gcatggcaaa actctttatt
     8581 tatcagtagt caaaacggta gcttgcggga cggatgtacc gaaacggttt ccgaccagga
     8641 aagccagcaa acttagtagt aacgagggca cgatagggtg gaagcccagg tactgaatat
     8701 tcagcgtcgc gagtacggca tacagcacgc cgccaacgat catcgcactt agcgcgcctt
     8761 tggcgttggc gcgttcccag taaagaccca gcaccagcgg ccacaggaaa acggcttcca
     8821 gcccaccgaa ggccagcaaa ttcagccaga tgatcatttc tggcggcttc caggcggcaa
     8881 gcagcagcaa cgcgccgaga actaacgtaa ttaccgccga catccgcttc agacgcgtct
     8941 cgttttgcat ttgatccgga cggatattca gatagagatc tttaatgatc gtagcggaac
     9001 tttgtagcaa ttgggcgtta attgtcgaca tgatcgcagc cataggtgca gccagaaaga
     9061 tcccggcagc aaacggtggc agcactttta ccattaacgt cgggatcacc agatccggta
     9121 cggtgagatc ggggatcact gcacgaccta acgctccggc taggtgcata ccgaacatca
     9181 ggattgcgac tacaatcgta ccgatgatga tcccccggtg taccgctttg ctgtctttat
     9241 aagagataca gcgcaccgca gtatgcggca ggccaatcac gccaaagcac accagtaccc
     9301 agaacgacgt cataaaggca ggcgacagaa tatcgtcagc gccttgtggc gtaaccagtt
     9361 gcggatcgat ggtttgcaag gtctgtactg cgttgcttaa gccgccagcg gcatgtacca
     9421 cgccaataag cagcacaacg gtgccaatca gcatcacaag cccttgcatg gtgtcgttca
     9481 gcacgctggc gcgaaagccg ccaaaggcgg tatacaacgc aatgctgata ccgaaaatca
     9541 gcagcccggt ttcataagga atacccgccg cggtttccag caggcgcgca ccgccgataa
     9601 actgcacggt cattgcccca acgaacgcaa ccagcaaact caaactcgcc agccacacca
     9661 gaagacgact ctggtagcgg gcaaacagca tgtcgttcag cgtcactgca ttgtagcggc
     9721 gcgcaagaat cgcaaacttc ttgccgagaa taccgagtga aagccagact gcaggaagct
     9781 gaatcatcgc cagcaatacc cagcccagcc cgtatttata agcagctcct ggcccgccga
     9841 taaacgaact ggcactgata taggtcgcgg tgagcgtcat cgccagcaca ataccgccca
     9901 tagagcggct gccgaggaaa tactcattaa ggaaggtgcc ggtgctccgt ttacgcatcg
     9961 cataaaccga gataccgaac accaccacca gataggcgac cagcggtaga attacttcaa
    10021 gctgcatcgt catcctccag tgggatatcg cgatagataa atttcaccat cgcccagcac
    10081 agtccaataa acagtagcgg cgtcaggatg caggccatct caaaccagcg cggaaagccg
    10141 gtaaaaccgg gggcaacgcc aggtaagtaa gcggctacta accaaactgc cagatacaaa
    10201 agggtcagcc ccagcgccca gcgcgcctct ttatgggcct gaacaaaacg agtgtccatt
    10261 ttttgtccct gatgggtgaa gaaagcgagg attgtacctt atgggggttg tcgatcccca
    10321 gtaataaaaa aggccggaaa atccggcctt ttggcgcttt tgcagtctta tttttcctga
    10381 agaccgagtt ttttctccag atagtggatg ttagtgccac catgctggaa gttctcgtca
    10441 ttcatgatgc ggatctgcag atcaacgttg gttttgatac cgtcgatgat cagctcctgc
    10501 agcgcattct tcatgcgggc aatcgccacg tcacggtttt caccgtagca aatcagctta
    10561 ccgatcattg agtcatagta cggcggtacg gtgtagcccg cgtagatatg agactcccaa
    10621 cgtacgccaa aaccgccagg tgcgtggaaa cgggtgattt tgcctgggct tggcaggaag
    10681 gtgttcggat cttcggcgtt gatacgacat tccaccgcat ggccgcgaac gtgaacttct
    10741 tcttgcttga tcgacagcgg ttgaccggca gcgatacgga gctgttcttt gatcaggtca
    10801 acgccagtga tcatttcggt aaccgggtgt tctacctgaa tacgggtgtt catttcgatg
    10861 aaatagaact cgccgttttc gaacagaaac tcgaaagtac ctgcaccgcg atagccgata
    10921 tcaacacacg ctttagcgca acgttcgccg atgtagcgac gcagttccgg ggtaatgccc
    10981 ggtgctggcg cttcttcgac cactttctgg tggcggcgct gcatggagca gtcacgttcc
    11041 gccagataga tagcgttgcc ccgaccgtca gccagtacct gaatctcgac gtggcgagga
    11101 ttttccaggt atttctccat gtaaaccata tcgttgctga aagcagcttt cgcttccgca
    11161 cgggtcatgg agatggattg tgccagttca gcgtcgccgc gcactacgcg cataccgcga
    11221 ccaccgccgc cgccggaggc tttgataatc accggataac caatgcgttt agcaatggca
    11281 cggtttttat ccatatcgtc gcccaacggg ccgtcagaac ccggtacgca agggacgccc
    11341 gcttttttca tcgccgcgat tgcggatact ttgtcgccca tcaggcgaat ggtttctgct
    11401 ttcgggccga tgaagataaa gccggagcgt tcaacctgct cggcaaagtt ggcgttctcg
    11461 gagaggaagc cgtaacccgg atggattgct actgcgccgg tgatttcagc ggcgctgatg
    11521 attgccggga tgttcagata actttttact gacggagcag ggccaataca gaccgtttca
    11581 tctgccagta atacgtgttt tagatcgcga tccgcgctgg agtgcacagc gacagtcttg
    11641 atgcccagtt ctttacaggc acgaagaata cgcaatgcaa tctcgccgcg gttggcaata
    11701 acaattttat ccagcatgtt cgcctcgtta ctcgatgacg accagcggct cgtcaaattc
    11761 taccggttgt ccactttcga ccagaattgc tttcacggta ccggatttgt ccgcttcgat
    11821 ctggttcatc attttcatgg cttcaacgat gcacagggta tcgcccacgt tgactttctg
    11881 acccacttcg atgaacgctt ttgcgtccgg gcttggggtg cggtagaaag taccaaccat
    11941 cggggaacgt acgatgtgac cactgatttc cgctgctgct ggcgcttcca tggaaggaac
    12001 ggtcgccgga gcggctgcgt tagattgagc tggctgctgc atcattggtg cagcgtaagc
    12061 ttgttgcatc acagggaaac ttgcggcagg agctgcacgg ctaatgcgta ctgactcttc
    12121 gccttcagaa atttccagtt cggagatgcc tgattcttca accagctcga tcagtttttt
    12181 aatcttacga atatccatga gtgggttccg tactctttgt ttagtgtgat tgtgacaggc
    12241 gtttcaccgc cgtctgtaaa gcgtatgccc cgacagctgt atgcatagcg ataaattcca
    12301 gcaggccggg acgccggtct attttgcccc agagtatcaa tgttagactt gacggacatt
    12361 gtgcagcgat ctgctgctat cctccggcaa aaaacaaaat ataccttcgt tgcgccaatg
    12421 tcaccttttc cgcagcgcaa aaactgcgtc agggaggacg aggtcgcaca ttataacgat
    12481 ttcgtagcaa ttggcagcta aatactggtc tcatcaggga agataatcaa cagctaacat
    12541 gtaaataacc ttcaacaccg tgtaatttgc aacaagccgc acaattcacg aaattagcgc
    12601 catcatcgac ggaacttctt ataacgtaag gtgaaaagct gtaaagccag ccctgcgaag
    12661 atgaggggct gcaatgagat aatcttcaca gaccacaaat aatgtaggga tgccaggatc
    12721 gcgacaagat agacgacatt acgcagcggt tgctggggat tacccaattt tcactgcatc
    12781 gtctggatag atgtgaatgc taaagcacgc aaaatcaacc gactgacaat tctcagcatt
    12841 aaataggttc gagtggttaa tttgtagtca aataaaatca gattattcac cccatggtac
    12901 cagaaatcag taacaggtta agcgtagtgt cgcccaggca aagcaccata aacctaacag
    12961 gcgacggagg cgtatcaata atggctgttt agaggagtat caatgcgcat caatttgtgg
    13021 tcagtcgaaa tcatgacttt ccagtttgga tgatgttgta caacttcctg accaaggtgt
    13081 tcgacgaaag ctgtccggct aaccgctttt cccgttaacc gattgaataa tgatgtcaaa
    13141 atgagttccc tctcttatta ttccttgcta aatggttagt taaccttcac cagcgtgcga
    13201 ccctggatct ggttattaat gatggcctcg gcaaagttcg gtgcctctga cagagatatc
    13261 tcttttgccg cctgggtata gaatgattcc ggtaaatcgg cgaccagtcg ctgccaggct
    13321 tgtgcgcggc gttctggtgg cgtcattact gaatccaccc cttgcaaacg gacattacgc
    13381 agaataaatg gcatgaccgt ggttggcaga gtaaaaccac ccgccagacc acaggccgcc
    13441 acgcagccgc cgtaattcat ttgcgccagc acttttgcca gcactttgtc gccaacagtg
    13501 tcaattgccc cagcccagac ttgtttttcc agaggacggg attcggcaaa ctcatcacga
    13561 gggagaatac ggctggcacc taaacttttc agatattcat gggtactttc gcgaccggaa
    13621 acggcaacga cctgataacc caacttatgc agcagcgcca cggcggtact gccgacgcca
    13681 ccactggcac ccgtcacgac aatctccccg tcctgcgggc gaacaccggc atcttccagc
    13741 gccatcacac acagcatggc ggtaaaaccg gcagtaccga taatcattgc tttacgcgcg
    13801 tccagccctt gcggcatggc aaccagccag tcacctttca ctcgcgcctg ctccgccagc
    13861 ccaccccagt ggttttcacc aacgccccag ccagtgagta acacctcctg accggcatga
    13921 aaacgcggat cttcgctggt gcgtacagtt ccggcaaaat cgatcccagg aatcatcgga
    13981 aaattacgga tgatttttcc cttaccggta atcgccagcg catctttata gttcaggctc
    14041 gaccagtgaa catcgaccgt gacatcgccc tccggcaggc gactttcgtc cagagtctgt
    14101 actgatgcga gagttttgcc gtcctgctgt tctaaaagta acgcctgcat aagtggtcct
    14161 catgtgcatg atggattgga aaataatttc tgaagattat actcgctaat ggaaaagcaa
    14221 agccgatgaa gcgcaataaa ttgcgagata aatctgattt gctagtatgc ccgcttcctc
    14281 actatcggag ttaacacaag gatgagatta acgacgaaat tttcggcctt tgttacgctg
    14341 ctcaccgggt taacaatttt tgtgactttg ctgggctgtt cgctaagttt ctacaacgca
    14401 attcagtata agtttagtca tcgcgttcag gcggtggcga cggcgattga tacccacctt
    14461 gtgtcgaatg acttcagcac attaaggcca caaattaccg aattaatgat gtcggcagat
    14521 atcgttcgtg tagacctgct ccatggtgat aaacaggttt ataccctggc cagaaatggt
    14581 agttatcgtc cggttggctc cagcgatctg tttcgcgaac tgagtgttcc gttgataaag
    14641 catccgggga tgtcgctgcg tctggtttat caggatccga tgggcaacta tttccattct
    14701 ttaatgacta ccgcgccgct cacgggggcg attggcttta tcattgttat gctcttcctg
    14761 gcggtacgct ggttacaacg gcaacttgcc gggcaagaat tgctggaaac ccgggctact
    14821 cgtatcttaa acggtgagcg tggctctaat gtgttgggaa ccatctatga atggccgccc
    14881 agaaccagca gtgcgctaga tacgctgctt cgtgaaattc agaacgcacg cgaacaacac
    14941 agccgtcttg atacgctgat ccgctcttat gccgcccagg acgtgaaaac cggcctcaat
    15001 aaccgactct ttttcgataa tcagttagca acgttactgg aagatcagga gaaagtaggt
    15061 acccacggga tcgtgatgat gattcgtctg ccggatttca atatgttgag cgacacttgg
    15121 gggcacagcc aggttgaaga acagttcttc actctgacta atctgctgtc gacatttatg
    15181 atgcgctacc ctggcgcact gctggcgcgt taccaccgca gtgattttgc tgcgcagtta
    15241 ccgcaccgga cgttaaaaga ggcagagagc atcgccggtc agttaatcaa agccgtcgat
    15301 accttgccga acaataaaat gctcgatcgc gacgatatga tccacattgg tatctgcgcc
    15361 tggcgtagtg gtcaggatac cgagcaggta atggaacatg cagagtctgc cacgcgtaat
    15421 gcgggattgc agggcggcaa tagctgggct atttacgatg actcgttgcc tgaaaaagga
    15481 cgcggtaatg ttcgctggcg tacgcttatc gagcaaatgc tcagtcgcgg cggcccgcgc
    15541 ctttatcaaa aaccggcggt tactcgcgaa ggtcaggttc atcatcgcga actcatgtgc
    15601 cgcatcttcg atggtaatga agaggttagc tcggcggagt atatgccgat ggtcttgcag
    15661 tttggcttat cggaagagta tgaccgtctg caaatcagcc gtcttattcc actattgcgt
    15721 tactggccag aggaaaatct ggcgattcag gttaccgttg agtcgctgat tcgcccgcgt
    15781 tttcagcgtt ggctgcgcga tacgttaatg caatgtgaaa aatcacaacg aaaacgcata
    15841 attattgaac ttgcagaggc cgatgtaggt caacatatca gtcgtttaca acctgttatt
    15901 cgtttagtga atgctttagg ggtacgggta gccgtcaacc aggctggttt gacgctggta
    15961 agcaccagtt ggatcaaaga acttaatgtt gagttactca agctccatcc ggggctggtc
    16021 agaaacattg agaagcgaac ggagaaccag ctgctggttc aaagcctggt ggaagcctgc
    16081 tccgggacca gcacccacgt ttacgccacc ggcgtgcgtt cgcgaagcga gtggcagacc
    16141 ctgattcagc gcggtgttac aggcgggcaa ggggattttt tcgcgtcctc acagccactt
    16201 gatactaacg tgaaaaaata ttcacaaaga tactcggttt aacctgccgt ttaatccgtt
    16261 ttcacgtaga ataacgcgcg ctgcgtctca tgggagtgtg cttgtctgct cgccagattg
    16321 ttgcagcaca tatgcagatg aatgacctta cgcggttgca aacaggcgag gaatgctgct
    16381 gatgcattaa gcctttctgg actcaggcag agatttgtaa caaaggaaac gaactgcact
    16441 aattttcacc gtagcagatg atttttgcgc cttgtcgctg ctgcgtgtgg ttggtaaagt
    16501 aagcggattt tcttttccgc cccagctttc aggattatcc cttagtatgt tgaaaaaatt
    16561 tcgtggcatg ttttccaatg acttgtccat tgacctgggt actgcgaata ccctcattta
    16621 tgtaaaagga caaggcatcg tattgaatga gccttccgtg gtggccattc gtcaggatcg
    16681 tgccggttca ccgaaaagcg tagctgcagt aggtcatgac gcgaagcaga tgctgggccg
    16741 tacgccgggc aatattgctg ccattcgccc aatgaaagac ggcgttatcg ccgacttctt
    16801 cgtgactgaa aaaatgctcc agcacttcat caaacaagtg cacagcaaca gctttatgcg
    16861 tccaagcccg cgcgttctgg tttgtgtacc ggttggcgcg acccaggttg aacgccgcgc
    16921 aattcgtgaa tccgcgcagg gcgctggtgc ccgtgaagtc ttcctgattg aagaaccgat
    16981 ggctgccgca attggtgctg gcctgccggt ttctgaagcg accggttcta tggtggttga
    17041 tatcggtggt ggtaccactg aagttgctgt tatctccttg aacggcgtgg tttactcctc
    17101 ttccgtgcgc attggtggtg atcgtttcga cgaagctatc atcaactatg tgcgtcgtaa
    17161 ttacggttct ctgatcggtg aagccaccgc agaacgtatc aagcacgaaa tcggttcggc
    17221 ttacccgggc gatgaagtcc gtgaaatcga agttcgtggc cgtaacctgg cagaaggtgt
    17281 tccacgcggt tttaccctga actccaacga aatcctcgaa gcactgcagg aaccgctgac
    17341 cggtattgtg agcgcggtaa tggttgcact ggaacagtgc ccgccggaac tggcttccga
    17401 catctccgag cgcggcatgg tgctcaccgg tggtggcgca ctgctgcgta accttgaccg
    17461 tttgttaatg gaagaaaccg gcattccagt agttgttgct gaagacccgc tgacctgtgt
    17521 ggcgcgcggt ggcggcaaag cgctggaaat gatcgacatg cacggcggcg acctgttcag
    17581 cgaagagtaa tcggatgcag gcaggggaaa tgtctgttta ccctgcctgg tctgatacga
    17641 gaatacgcat aacttatgaa gccaattttt agccgtggcc cgtcgctaca gattcgcctt
    17701 attctggcgg tgctggtggc gctcggcatt attattgccg acagccgcct ggggacgttc
    17761 agtcaaatcc gtacttatat ggataccgcc gtcagtcctt tctactttgt ttccaatgct
    17821 cctcgtgaat tgctggatgg cgtatcgcag acgctggcct cgcgtgacca actagaactt
    17881 gaaaaccggg cgttacgtca ggaactgttg ctgaaaaaca gtgaactgct gatgcttgga
    17941 caatacaaac aggagaacgc gcgtctgcgc gagctgctgg gttccccgct gcgtcaggat
    18001 gagcagaaaa tggtgactca ggttatctcc acggttaacg atccttatag cgatcaagtt
    18061 gttatcgata aaggtagcgt taatggcgtt tatgaaggcc agccggtcat cagcgacaaa
    18121 ggtgttgttg gtcaggtggt ggccgtcgct aaactgacca gtcgcgtgct gctgatttgt
    18181 gatgcgaccc acgcgctgcc aatccaggtg ctgcgcaacg atatccgcgt aattgcagcc
    18241 ggtaacggtt gtacggatga tttgcagctt gagcatctgc cagcgaatac ggatattcgt
    18301 gttggtgatg tgctggtgac ctccggtctg ggcggtcgtt tcccggaagg ctatccggtc
    18361 gcggttgtct cttccgtaaa actcgatacc cagcgcgctt atactgtgat tcaggcgcgt
    18421 ccgactgcag gactgcaacg tttgcgttat ctgctgctgc tgtggggcgc ggatcgtaac
    18481 ggcgctaacc cgatgacgcc ggaagaggtg catcgtgttg ctaatgaacg tctgatgcag
    18541 atgatgccgc aggtattgcc ttcgccagac gcgatggggc caaagttacc tgaaccggca
    18601 acggggatcg ctcagccgac tccgcagcaa ccgacgacag gaaatgcagc tactgcgcct
    18661 gctgcgccca cacagcctgc tgctaatcgc tctccacaaa gggctacgcc gccgcaaagt
    18721 ggtgctcaac cgcctgcgcg tgcgccggga gggcaatagt ggcgagctat cgtagccagg
    18781 gacgttgggt aatctggctc tctttcctca ttgcgctgtt gctgcaaatc atgccctggc
    18841 cggataacct gattgttttc cggccaaact gggtgttact catcttgttg tattggatcc
    18901 tggccttgcc tcatcgcgta aatgtgggca caggttttgt gatgggtgcc atactggatc
    18961 tgatcagcgg ctcgacgctt ggcgtacgcg tattggcgat gagcatcatt gcttacctgg
    19021 tggcgctgaa ataccagctt ttccgcaacc tcgcattatg gcagcaggcg ctggtcgtca
    19081 tgttgctttc gctggtggtg gatattattg ttttctgggc agagttttta gtgattaacg
    19141 tctctttcag accggaagtg ttctggagta gtgtagtcaa tggggtgctc tggccgtgga
    19201 ttttcttgct gatgcgtaaa gtccgtcagc agtttgcagt gcaataaagg tttctatgac
    19261 ttctctgtat ttagcttccg gttctccgcg tcgtcaggag ttacttgcgc aacttggtgt
    19321 gacctttgaa cgtattgtta cgggcattga ggagcagcgt cagccgcagg agagcgcgca
    19381 gcagtatgtt gtgcgtctgg cgcgcgagaa agcacaggcg ggtgtcgcgc aaacggcgca
    19441 ggatctcccg gtgctgggtg cggatactat cgttatcctg aatggagaag tgctggagaa
    19501 accgcgcgac gcagagcatg cggcgcagat gttgcgcaaa ttatcgggtc agacccatca
    19561 ggtgatgacg gcagtggcgt tggccgacag ccagcacatt ctcgattgcc tggtggtcac
    19621 cgatgtgact ttcagaacgt taacagacga agacatcgcg ggctatgtcg ccagcggtga
    19681 accgttagat aaagcaggtg catacggtat tcaggggctg ggtggctgtt ttgtcaggaa
    19741 gataaatggc agctatcacg ccgtagtcgg cttacctctg gttgaaacgt atgaattatt
    19801 aagtaatttt aacgcactgc gtgagaaaag ggataaacat gacggctgaa ttgttagtaa
    19861 acgtaacgcc ttcggaaacg cgagtggcgt atattgatgg cggtattctg caggaaattc
    19921 atattgaacg tgaggcgcga cgcggaatag taggcaatat ctacaagggt cgtgtaagtc
    19981 gtgtacttcc gggtatgcag gcggcttttg tagatattgg gctggataaa gccgcgtttc
    20041 ttcatgcatc cgatatcatg ccgcacaccg aatgtgtggc gggtgaagaa caaaagcaat
    20101 tcacggtgcg cgacatctcg gaactggttc gtcaggggca agatctgatg gtgcaggtgg
    20161 tgaaagatcc gcttggcact aaaggtgcgc gcctgaccac cgatatcacg ctgccttctc
    20221 gctatctggt ctttatgcca ggggcttctc acgttggggt ttcccaacgt attgaaagcg
    20281 aatcagaacg tgaacgcctg aaaaaagtgg tcgcagagta ttgcgacgag cagggcgggt
    20341 ttatcatccg taccgcagcg gaaggggttg gcgaggctga actggcctcc gatgccgctt
    20401 atctgaaacg cgtctggacc aaagttatgg agcgtaaaaa gcgcccgcag acccgttatc
    20461 agctgtacgg cgaactggcg ctggctcagc gtgttctgcg tgatttcgcc gacgccgaac
    20521 tggaccgcat tcgcgttgac tcacgcctga cttacgaagc attacttgag ttcacctcgg
    20581 agtacattcc cgagatgacc agcaagctgg agcattacac cggacgccag ccgattttcg
    20641 atctctttga tgtcgaaaac gaaatccagc gagcgctgga acgcaaagta gaactgaaat
    20701 ccggtggtta tctcattatc gaccagaccg aagcgatgac caccgtggac atcaataccg
    20761 gagcgtttgt cggtcatcgc aatctggacg acaccatttt caataccaat attgaagcga
    20821 cgcaggctat cgctcgccag ttacggttgc gtaatctggg cgggattatc attattgatt
    20881 tcatcgatat gaataatgaa gatcaccgcc gccgcgtgct gcactcgctg gagcaggcgt
    20941 tgagcaaaga ccgggtgaaa accagcgtta atggtttttc ggcgctgggg ctggtggaga
    21001 tgacgcgtaa acgcacccgc gaaagcattg agcacgtact gtgtaacgaa tgcccaacct
    21061 gccacggtcg cggaacggtg aaaaccgtgg aaacggtatg ctatgaaatc atgcgcgaga
    21121 ttgttcgtgt ccaccatgct tacgactccg accgtttcct ggtctatgct tctccggcag
    21181 tagccgaagc cttgaaaggc gaagagtcac actcgctggc ggaagtggaa attttcgttg
    21241 gcaaacaggt taaagtacaa attgaaccgc tctataacca ggagcagttt gacgtcgtaa
    21301 tgatgtaaac agatgctggg ccgccatccg gcaaagggtt tttgagtcac atttttagca
    21361 gacaaggagt gacgggtgag gcgattgccg gggattttac tgcttactgg agccgcgctc
    21421 gttgtgatcg ctgccctgct ggttagcggc ctgcgtattg ctttaccgca tcttgacgcc
    21481 tggcgtccgg aaatcctcaa caaaatagaa tccgcgactg gcatgccggt agaagccagt
    21541 cagctctcag ccagctggca gaattttggc ccgacgcttg aagcacacga catccgtgca
    21601 gaactaaaag atggcggcga attttcggtt aaacgcgtta ctctggcgct ggatgtctgg
    21661 cagagcctgt tacatatgcg ctggcagttt cgcgacctca ctttctggca gctgcgcttt
    21721 cgcaccaaca ctcctatcac cagcggtggt agcgatgaca gtctggaagc cagtcacatc
    21781 agcgatctgt ttcttcgtca atttgaccat ttcgatctcc gcgacagtga agtcagtttc
    21841 ctgacgccat ccggtcagcg tgccgagctg gcgatcccac aactcacctg gctgaacgat
    21901 ccacgtcgac accgtgcgga aggcctggta agcctctcca gccttaccgg acagcacggc
    21961 gtgatgcagg tgcgcatgga tttgcgcgat gatgaggggt tgttaagcaa tggtcgcgtc
    22021 tggctacagg cggatgacat cgacctgaag ccgtggctcg gtaaatggat gcaggacaat
    22081 attgctctgg aaacggcaca gttctccctt gaaggctgga tgacgatcga caaaggcgat
    22141 gtaaccggcg gtgacgtctg gctgaaacag ggcggtgcca gctggttggg cgagaagcaa
    22201 acgcatacgc tgtcggtgga taatctgacc gcgcatatta cgcgtgaaaa tccgggctgg
    22261 cagttctcta ttcccgatac acggatcacg atggacggca aaccctggcc gagcggagca
    22321 ttgacgctgg cctggatacc ggaacaggac gttggcggca aagacaataa acgcagtgac
    22381 gaactccgga ttcgcgccag taatctggag ctggcaggcc tggagggcat acgcccgctg
    22441 gccgcgaaac tttcacctgc actgggtgat gtttggcgct ccacacaacc gagcggcaag
    22501 attaacactc tggcgctgga tatcccgctt caggcggcag acaagacccg ttttcaggca
    22561 tcgtggagcg atctggcctg gaagcaatgg aaattattac cgggtgcgga acacttctcc
    22621 gggacgcttt ccggcagcgt tgaaaatggt ttgcttaccg cgtcgatgaa gcaggcaaag
    22681 atgccttacg aaacggtatt ccgtgcgcca ctggaaatcg ccgacggcca ggcaactata
    22741 agctggctga acaatgacaa aggtttccag ctggatgggc gtaatattga cgttaaagcc
    22801 aaagccgtcc atgcgcgcgg cggttttcgt tacctgcaac ctgctaacga tgaaccctgg
    22861 ctgggtattc tggctggcat cagtaccgat gatggttcac aagcctggca ctatttcccg
    22921 gaaaacttga tgggtaaaga cctggttgat tacttaagtg gcgcgattca gggcggtgaa
    22981 gcggataacg cgacgctggt ttatggtggc aatccgcaac tcttccccta taaacacaac
    23041 gaaggtcagt ttgaagtgct ggtgccgctg cgcaacgcga agtttgcctt ccagccggac
    23101 tggcctgcat taactaacct tgatattgaa ctggacttta ttaacgacgg tttatggatg
    23161 aaaaccgatg gcgttaatct gggcggcgtg cgcgcgagta atctcaccgc agtgatccct
    23221 gactactcga aagaaaaact gctgattgac gctgacatta aaggtccggg taaagccgtt
    23281 ggcccttact ttgatgagac accgctgaaa gattctctgg gtgcgaccct gcaagaactc
    23341 cagctcgacg gcgatgtgaa tgctcgctta catcttgata tcccgctgaa cggcgagctg
    23401 gtaaccgcga aaggtgaagt gacgctgcgt aataacagtc tgtttatcaa accactcgac
    23461 agcaccctga aaaatttgag cggtaaattc agctttatca atggcgatct gcaaagtgaa
    23521 ccactgacag caagctggtt taatcagccg ttgaacgtgg atttttccac caaagaaggg
    23581 gcaaaagcct accaggttgc ggtgaatctc aacggtaact ggcaaccggc gaaaaccggc
    23641 gttctgcctg aagcggtgaa cgaagcattg agtggcagcg tggcgtggga tggtaaagtg
    23701 ggcattgatc tgccttatca tgctggcgcg acctataacg tagagctgaa cggcgatctg
    23761 aataatgtga gtagtcactt accttcaccg ttagccaaac ctgcgggtga accactagcg
    23821 gtaaacgtta aggttgatgg caatctcaac agctttgaat taaccggaca ggctggtgcg
    23881 gataaccatt tcaatagccg ctggttgctt ggtcaaaagc tgacgctcga tcgtgctatt
    23941 tgggcggcag acagtaaaac gctcccgccg ttgccggaac aaagtggcgt tgaactcaat
    24001 atgccgccga tgaatggtgc cgagtggctg gccctgttcc agaaaggcgc tgcggagagt
    24061 gtcggtggtg cagcgagttt cccacaatac ataacgttac gtacgcctat gttgtcactg
    24121 ggaaatcagc aatggaataa cctgagtatt gtttcgcaac cgacggcaaa tggcaccctg
    24181 gttgaggcgc aagggcgtga aatcaacgcc acgctagcga tgcgtaataa cgcgccgtgg
    24241 ctggcgaata tcaaatatct ttattacaac ccgagcgtgg cgaaaactcg tggtgattca
    24301 acgccgtcat cacctttccc gacaacggag cgcattaact tccgtggctg gccggacgca
    24361 caaatacgat gcacagagtg ctggttctgg gggcaaaaat tcggtcgcat tgacagtgat
    24421 atcaccattt ctggcgatac gttaacgctg accaatggac tgattgatac tggtttctcg
    24481 cggcttactg ccgatggtga atgggttaat aatccgggga atgaacgtac ctcgctgaaa
    24541 ggaaaactgc gcgggcagaa aattgatgcc gccgcagaat tttttggtgt cacgacgccc
    24601 atacgccagt cgtcatttaa tgtggattac gatttacact ggcgcaaagc accctggcag
    24661 ccagatgaag cgacgttgaa tggcatcatt catactcaac tgggtaaagg cgaaattacc
    24721 gaaatcaata ccggacatgc cgggcaattg ctgcgcttat tgagcgtaga tgccctgatg
    24781 cgtaagctgc gttttgattt cagagacact tttggcgaag ggttctattt tgactccatt
    24841 cgcagcaccg cgtggattaa agacggggtt atgcacaccg acgacacgct ggtggatggc
    24901 ctggaggcgg atatcgccat gaaagggtcg gtaaatctgg tacgtcgcga cctgaatatg
    24961 gaagcggttg tcgcaccaga gatttctgcg acggtgggcg tggctgcggc ttttgcggtt
    25021 aaccccattg ttggcgcggc agtttttgcc gctagtaaag tgctggggcc gctgtggagc
    25081 aaagtctcca ttttgcgcta tcacatttcg ggtccgctgg acgatccgca aatcaacgaa
    25141 gtgttgcgcc aaccgcgtaa agaaaaagcg caatgatttg acgagggcgc gtaattgccc
    25201 caatctcata tgataatcgt tgccaaaggc caacgagcca gaacataacc gtaggccgga
    25261 tagggcgttc atgccgcatc cggcagccgt aaaaaatcct ctactgcagt aactaacgag
    25321 tagcaaaaac gatgagtctg aacctggtaa gtgaacaatt gctagcggcg aacagcctga
    25381 aacatcagga cttgttcgcg atcctcggtc aactggccga acgtcgcctt gattatggcg
    25441 atctctattt tcagtcgagc tatcacgaat cctgggtttt agaagaccgc attattaaag
    25501 atggttctta caacatcgat cagggcgttg gcgtgcgtgc aatcagcggt gaaaaaaccg
    25561 gatttgctta cgctgaccaa atcagcctgc tggcgctgga acagagtgcg caagcggcgc
    25621 gcaccatcgt ccgtgatagt ggtgatggca aagtacagac gctgggcgcg gtagagcata
    25681 gcccgttgta tacctcggta gatccgctgc aaagcatgag ccgtgaagag aagctggata
    25741 tcctgcgtcg cgtcgataag gttgctcgcg aagcggacaa gcgcgtacag gaagtgactg
    25801 ccagcctcag tggtgtctat gaattaattc tggttgcggc caccgacggc acgctagcgg
    25861 cggatgtccg tccgctggtg cgtctttccg tgagcgttct ggtcgaagaa gatggcaaac
    25921 gcgaacgcgg tgccagtggc ggcggcggtc gttttggtta tgagttcttc cttgccgatc
    25981 ttgacggcga agtccgtgcg gatgcatggg caaaagaagc agtgcgtatg gcgctggtca
    26041 atctttctgc cgttgctgca ccagcgggca ccatgccggt agtacttggc gcaggttggc
    26101 cgggcgtgct gttgcatgaa gcggtaggtc acggtctgga aggcgacttc aaccgccgcg
    26161 gcacttcagt atttagtggg caggtcgggg agctggtggc ttcagaactg tgtaccgtgg
    26221 ttgatgacgg cacgatggtc gatcgccgtg gttcggtggc gattgatgac gaaggtacgc
    26281 caggccagta caacgtgctg attgagaacg gcattctgaa aggctacatg caggataaac
    26341 tcaacgcgcg tttgatgggg atgacgccga ctggcaacgg tcgccgtgaa tcctacgccc
    26401 atctgcccat gccgcgtatg actaacacct atatgctgcc gggtaaatcg accccgcagg
    26461 aaattattga atccgttgag tacggtatct atgcaccgaa ctttggtggc ggtcaggtgg
    26521 atatcacctc cggcaaattc gttttctcca cttcagaagc atatctgatt gaaaacggta
    26581 aagtaacgaa gccggtgaaa ggcgcaacgt tgattggttc cggtatcgaa accatgcagc
    26641 agatttcgat ggttggcaac gacctgaaac tggataacgg cgtgggtgtc tgcggtaaag
    26701 aagggcaaag tttgccggtt ggcgtgggcc agccaacgct gaaagttgat aacctgactg
    26761 ttggcggtac tgcgtaataa tttcattatt ttcagaagga taattaatct ttctacgtgc
    26821 acgaacggtc ccctcgctcc tctggggtta gggtgagggg aacccgttgg cacaggtttg
    26881 tagaacgtaa cagtacaata tgaattactt ctctttcccg cgcccgtgca tctcctgaaa
    26941 caatttaccg acctcaacaa aataatccgt cagcgagttg atcacgacct gtaccttcag
    27001 cggcagctta tctttttcgg tatataacgc ataaaccggg cgtggatctg actgataacg
    27061 cggcagcagg atctccagct caccgcgatt gatctcgttg atcacccaca tcagcggcac
    27121 gtaggcgatc ccggcacccg ccgtcagcca gcgcaccagc gtcatcggat cattagtcac
    27181 aaatcttcct tgtgggatca ggcgagtcga gatcccttcc ggtgcgatca gttcaaattc
    27241 attgtcgggc cgcacgctgt attcaagcca tgaatgacta ctcaaatcgg cgggtttttc
    27301 cggtatgccg tattgtgtga gatagctttt cgcggcgcac accaccatcg gcatcgcgcc
    27361 cagacggcgg gaaaacaggc tggaattccg gtaaccagat tgacgctcaa acctgggtat
    27421 tctttcagca tttttgctgt cagcccggcg agaacatttt gtgccatagt tgaagaacag
    27481 ccaatgcgca gcgtcccgat gggggtgtta ttgaaggcat acagttgctc atgaacatcc
    27541 tgcacttcat gaagcatccg acggcagccc tggtagtaaa ttctgccggc ttcggtcagg
    27601 ccaatgctgc gtgtgctacg gtttaacagc tttacctgca actcatcttc cagttttgac
    27661 accgtctgac tgatggacga aacgctcatc tgtagctgtc tggcggcggc ggtaaaagag
    27721 ccaaattcaa ctactttggc aaacaccgac atgcgtttta gtcgttccat tattcactct
    27781 gacttaaaag tgatttagat cacataatat agataacagc ataacagtta cgctaatata
    27841 ttaaatatca atctacagca atgttgctct cgcccggctt tccatgccct tctctgcggc
    27901 gacagatgct gaaaataata acgcctgctc tctctttaca accaaggtca acatgagtct
    27961 gtttcccgtt atcgtggtgt ttggactgtc cttcccaccg atattttttg aattgctttt
    28021 atcactggcg attttctggc tggtgcgccg ggtacttgtg ccaacaggta tctacgactt
    28081 tgtctggcat ccggcgttgt tcaacaccgc gctctattgc tgcttgtttt atttgatatc
    28141 gcgactgttc gtttgaggtt gaagtgaaaa cactaataag aaaattctcc cgtacggcca
    28201 tcacggtcgt attagtcatt ctagccttca tcgcaatttt taatgcctgg gtctattaca
    28261 ccgaatcccc ctggacgcgt gacgcgcgct ttagcgctga cgtcgttgcg atcgcgccgg
    28321 acgtttctgg actcattacc caggtgaatg ttcatgataa ccagctggtg aaaaaaggac
    28381 agatactgtt caccatcgac cagccgcgct atcaaaaggc gcttgaggaa gcgcaagccg
    28441 atgttgctta ttatcaggta ctggcacagg agaaacgcca ggaggccgga cgtcgtaacc
    28501 gtctcggtgt gcaggcgatg tctcgcgaag agatcgacca ggccaacaac gtactacaaa
    28561 cggttctgca tcagttagcg aaagcgcagg cgacccgcga tctggcaaaa ctggatcttg
    28621 aacgcacggt gatccgcgcg ccagcagatg gctgggtgac caacctcaac gtctataccg
    28681 gtgagtttat tactcgagga tcaacggcgg ttgcgctggt gaaacagaac tccttctatg
    28741 tactggccta tatggaagaa actaagctgg aaggggggcg tccggggtat cgtgcagaga
    28801 tcacgccgct tggcagtaac aaagtgctga aagggactgt tgatagtgtt gccgcagggg
    28861 tcaccaacgc cagcagcacg cgtgacgaca aagggatggc gactatagac tctaatcttg
    28921 aatgggtgcg tcttgcgcaa cgtgttccgg ttcgtattcg tctcgacaac cagcaagaga
    28981 acatctggcc tgcgggcacc actgctacag tggtggtcac tggcaaacaa gatcgcgacg
    29041 aaagccagga ttcgttcttc cgtaaaatgg cccatcgcct gcgtgagttt ggttaatcac
    29101 gatgggtatt ttctccattg ctaaccaaca tattcgcttt gcggtaaaac tggcgaccgc
    29161 cattgtactg gcgctgtttg ttggctttca cttccagctg gaaacgccac gctgggcggt
    29221 actgacagcg gcgattgttg ccgccggtcc ggcctttgct gcgggaggtg aaccgtattc
    29281 tggcgctatt cgctatcgtg gctttttgcg catcatcggc acatttattg gctgtattgc
    29341 cggactggtg atcatcattg cgatgatccg cgcaccatta ttgatgattc tggtgtgctg
    29401 tatctgggcc ggtttttgta cctggatatc ctcgctggta cgaatagaaa actcgtacgc
    29461 gtgggggctg gccggttata ccgcgctgat cattgtgatc accattcagc cggaaccatt
    29521 gcttacgccg cagtttgccg tcgaacgttg tagcgagatc gttatcggta ttgtgtgtgc
    29581 gattatggcg gatttgctct tttctccgcg atcgatcaaa caagaagtag atcgagagct
    29641 ggaaagtttg ctggtcgcgc aatatcaatt aatgcaactc tgtatcaagc atggcgatgg
    29701 tgaagttgtc gataaagcct ggggcgacct ggtgcgacgc accacggcac tacaaggcat
    29761 gcgcagcaac ctgaatatgg aatcttcccg ctgggcgcgg gccaatcgac gtttaaaagc
    29821 gatcaatacg ctatcgctga cgctgattac ccaatcctgc gaaacttatc ttattcagaa
    29881 tacgcgcccg gaattgatca ctgatacttt ccgcgaattt tttgacacgc cggtagaaac
    29941 cgcgcaggac gtccacaagc agctcaaacg cctgcggaga gttatcgcct ggaccgggga
    30001 acgggaaacg cctgtcacca tttatagctg ggtcgcggcg gcaacgcgtt atcagcttct
    30061 caagcgcggc gttatcagta acacaaaaat caacgccacc gaagaagaga tcctgcaagg
    30121 cgaaccggaa gtaaaagtag agtcagccga acgtcatcat gcaatggtta acttctggcg
    30181 aaccacactt tcctgcattc tgggcacgct tttctggctg tggacgggct ggacttccgg
    30241 cagtggtgca atggtgatga ttgcggtcgt gacgtcactg gcaatgcgtt tgccgaatcc
    30301 acgcatggtg gcgatcgact ttatctatgg gacgctggcc gcgctgccgt tagggctgct
    30361 ctactttttg gtgattatcc ctaataccca acagagcatg ttgctgctgt gtattagcct
    30421 ggcagtgctg ggattcttcc tcggtataga agtacagaaa cggcgactgg gctcgatggg
    30481 ggcactggcc agcaccataa atattatcgt gctggataac ccgatgactt tccatttcag
    30541 tcagtttctc gacagcgcat tagggcaaat cgtcggctgt gtgctcgcgt tcaccgttat
    30601 tttgctggtg cgggataaat cgcgcgacag gactggacgt gtactgctta atcagtttgt
    30661 ttttgccgct gtttccgcga tgactaccaa tgtggcacgt cgtaaagaga accacctccc
    30721 ggcactttat cagcagctgt ttttgctgat gaataagttc ccaggggatt tgccgaaatt
    30781 tcgcctggcg ctgacgatga ttatcgcgca ccagcgcctg cgtgatgcgc cgatcccggt
    30841 taacgaggat ttatcggcgt ttcaccgaca aatgcgccgc acagcagacc atgtgatatc
    30901 tgcccgtagc gatgataaac gtcgtcggta ctttggccag ttgctggaag aactggaaat
    30961 ctaccaggaa aagctacgca tctggcaagc gccaccgcag gtgacggaac cggttcatcg
    31021 gctggcgggg atgctccata agtatcaaca tgcgttgacc gatagttaag tcaaaaccga
    31081 cgccaaaagc gtcggttttt tcatggctat acttagcgat gcacggcaga actcgccgcg
    31141 aaacgtgacg gtggcaacag atgaatattt atacctttga ttttgatgag attgagagtc
    31201 aggaggattt ttatcgtgac tttagccaaa cctttggtct ggctaaagat aaggtacgcg
    31261 atctcgactc actatgggat gtgttaatga acgatgtcct gccgctacca cttgagattg
    31321 aatttgttca tctgggagag aaaacgcgtc gccgttttgg cgcgttaata ttgctgtttg
    31381 atgaggcaga ggaagagctg gaagggcatt tgcgttttaa tgttcgtcat tagcgcaaaa
    31441 aaaagccccc gaaccggggg caatatcgtc ggacaagacg atgagggttt atttgtacag
    31501 ttcagccgta gcgtgccagg tgtcaccgct acgagcttca gtaatctggt aggccgttgc
    31561 gcctttctct tccgcttttt tgttcagcat ttcacgcata tccattggcg aagacgccac
    31621 accacttacg gatacggtcc cgattgcttc acgattttgt gcttgtgcag tatcaatgga
    31681 gtcggcagcg aatgcaccga aagagagaac agaaagtacg cttaatgcag ctacagtggt
    31741 tttgattttc atgattttta cctcgacata atcttttagc tgggcctttg tttcgtgatc
    31801 catatcacaa aatcaagcat acactaatta ttgaactaat taataccaca ctaactgttt
    31861 cagttgatac taagtgttaa aaatgtgaat tttaataaca aaaaagaatt aaaaacacgg
    31921 ttatttattg ttaattctag tattttcaat tagataatga attttacata atgtgctttt
    31981 tttaccgtat gtattcattg tgtgaatgat atgtcgcggt aaaacgcact attcgttaaa
    32041 ttaatcgcag gggaaagcag ggggttgaga ggaataagca acattttccc cgccgccaga
    32101 agcgacgggg cagagattaa agctcctggt cgaacagctc taaaatcgct tcgtacaggt
    32161 ctttgacggt gaaaccgtta gcaggggtgg taaagatggt gtcatcgcca gcgatggtgc
    32221 ccagaatacc ttctgctttg cccagtgagt ccagcaggcg agcaattaac tgcgccgcgc
    32281 cagggctggt atgaatcacg acaactgcat cgttgtagtc gatatccagc accagattct
    32341 tcaatggact ggaggtggtt ggtacaccca gttcagctgg caggcagtaa accatttcca
    32401 ttttggcatt gcgtgtacgt acagcaccaa acttggtcaa catccgcgag actttagact
    32461 gattaatatt gtcaaagcct tgctcctgca acgcggcgac gatttcgccc tgggagctaa
    32521 atttctcttc tttaagtaat gctttaaatg ctttaactag ttcttcttgc ttagccgagc
    32581 ttcgcataag tcacccgata tggtggttga tacaacatta ttgtgcatac agatgaattt
    32641 ttatgcaaac agtcagccct gaagaaggct gaaataatgt tatgaaagag cgggatttta
    32701 tcaaatttcg ttattgagaa acatgcctgc gtcacggcat gcaaattctg tttaaaagta
    32761 aattaattgt tatcaaattg atgttgtttt ggctgaacgg tagggtatat tgtcaccacc
    32821 tgttggaatg ttgcgctaat gcataagcga ctgttaatta cgtaagttag gttcctgatt
    32881 acggcaatta aatgcataaa cgctaaactt gcgtgactac acattcttga gatgtggtca
    32941 ttgtaaacgg caattttgtg gattaaggtc gcggcagcgg agcaacatat cttagtttat
    33001 caatataata aggagtttag gatgaaagtc gcagtcctcg gcgctgctgg cggtattggc
    33061 caggcgcttg cactactgtt aaaaacccaa ctgccttcag gttcagaact ctctctgtat
    33121 gatatcgctc cagtgactcc cggtgtggct gtcgatctga gccatatccc tactgctgtg
    33181 aaaatcaaag gtttttctgg tgaagatgcg actccggcgc tggaaggcgc agatgtcgtt
    33241 cttatctctg caggtgtagc gcgtaaaccg ggtatggatc gttccgacct gtttaacgtt
    33301 aacgccggca tcgtgaaaaa cctggtacag caagttgcga aaacctgccc gaaagcgtgc
    33361 attggtatta tcactaaccc ggttaacacc acagttgcga ttgctgctga agtgctgaaa
    33421 aaagccggtg tttatgacaa aaacaaactg ttcggcgtta ccacgctgga catcattcgt
    33481 tccaacactt ttgttgcgga actgaaaggc aaacagccag gcgaagttga agtgccggtt
    33541 attggcggtc actctggtgt taccattctg ccgctgctgt cacaggttcc tggcgttagt
    33601 tttaccgagc aggaagtggc tgatctgacc aaacgcatcc agaacgcggg tactgaagtg
    33661 gttgaagcga aagccggtgg cgggtctgca accctgtcta tgggccaggc agctgcacgt
    33721 tttggtctgt ctctggttcg tgcactgcag ggcgaacaag gcgttgtcga atgtgcctac
    33781 gttgaaggcg atggtcagta cgcccgtttc ttctctcaac cgctgctgct gggtaaaaac
    33841 ggcgtggaag agcgtaaatc tatcggtacc ctgagcgcat ttgaacagaa cgcgctggaa
    33901 ggtatgctgg atacactcaa gaaagatatc gccttgggcg aagagttcgt taataagtaa
    33961 ttgattagcg aataataaaa aaccggagca cagac
//
LOCUS       DAJVRF010000031        32632 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJVRF010000031 DAJVRF010000000
VERSION     DAJVRF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32632
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.031"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            424..744
                     /gene="cyaY"
                     /locus_tag="OQN13_001348"
     CDS             424..744
                     /gene="cyaY"
                     /locus_tag="OQN13_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCS1614746.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(784..3330)
                     /gene="cyaA"
                     /locus_tag="OQN13_001349"
     CDS             complement(784..3330)
                     /gene="cyaA"
                     /locus_tag="OQN13_001349"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCS1614747.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            3717..4658
                     /gene="hemC"
                     /locus_tag="OQN13_001350"
     CDS             3717..4658
                     /gene="hemC"
                     /locus_tag="OQN13_001350"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCS1614748.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4655..5395
                     /gene="hemD"
                     /locus_tag="OQN13_001351"
     CDS             4655..5395
                     /gene="hemD"
                     /locus_tag="OQN13_001351"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCS1614749.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            5417..6658
                     /gene="hemX"
                     /locus_tag="OQN13_001352"
     CDS             5417..6658
                     /gene="hemX"
                     /locus_tag="OQN13_001352"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS1614750.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPAPQGDTPAAAPQGE"
     gene            6661..7857
                     /gene="hemY"
                     /locus_tag="OQN13_001353"
     CDS             6661..7857
                     /gene="hemY"
                     /locus_tag="OQN13_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCS1614751.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            8519..8680
                     /locus_tag="OQN13_001354"
     CDS             8519..8680
                     /locus_tag="OQN13_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614752.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(8764..9999)
                     /locus_tag="OQN13_001355"
     CDS             complement(8764..9999)
                     /locus_tag="OQN13_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS1614753.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(10146..10222)
                     /locus_tag="OQN13_001356"
     tRNA            complement(10146..10222)
                     /locus_tag="OQN13_001356"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10186..10188),aa:Pro,seq:tgg)
     gene            complement(10265..10351)
                     /locus_tag="OQN13_001357"
     tRNA            complement(10265..10351)
                     /locus_tag="OQN13_001357"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10315..10317),aa:Leu,seq:cag)
     gene            complement(10372..10447)
                     /locus_tag="OQN13_001358"
     tRNA            complement(10372..10447)
                     /locus_tag="OQN13_001358"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10412..10414),aa:His,seq:gtg)
     gene            complement(10506..10582)
                     /locus_tag="OQN13_001359"
     tRNA            complement(10506..10582)
                     /locus_tag="OQN13_001359"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10546..10548),aa:Arg,seq:ccg)
     gene            complement(10683..12068)
                     /locus_tag="OQN13_001360"
     CDS             complement(10683..12068)
                     /locus_tag="OQN13_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1614754.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(12259..12999)
                     /gene="rffM"
                     /locus_tag="OQN13_001361"
     CDS             complement(12259..12999)
                     /gene="rffM"
                     /locus_tag="OQN13_001361"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCS1614755.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(13002..14354)
                     /gene="wzyE"
                     /locus_tag="OQN13_001362"
     CDS             complement(13002..14354)
                     /gene="wzyE"
                     /locus_tag="OQN13_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCS1614756.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(14351..15430)
                     /gene="rffT"
                     /locus_tag="OQN13_001363"
                     /gene_synonym="wecF"
     CDS             complement(14351..15430)
                     /gene="rffT"
                     /locus_tag="OQN13_001363"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCS1614757.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(15427..16677)
                     /gene="wzxE"
                     /locus_tag="OQN13_001364"
     CDS             complement(15427..16677)
                     /gene="wzxE"
                     /locus_tag="OQN13_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCS1614758.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(16679..17809)
                     /gene="rffA"
                     /locus_tag="OQN13_001365"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(16679..17809)
                     /gene="rffA"
                     /locus_tag="OQN13_001365"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCS1614759.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(17814..18488)
                     /gene="rffC"
                     /locus_tag="OQN13_001366"
                     /gene_synonym="wecD"
     CDS             complement(17814..18488)
                     /gene="rffC"
                     /locus_tag="OQN13_001366"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCS1614760.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAQLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(18466..19347)
                     /gene="rfbA"
                     /locus_tag="OQN13_001367"
     CDS             complement(18466..19347)
                     /gene="rfbA"
                     /locus_tag="OQN13_001367"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCS1614761.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(19366..20433)
                     /gene="rffG"
                     /locus_tag="OQN13_001368"
     CDS             complement(19366..20433)
                     /gene="rffG"
                     /locus_tag="OQN13_001368"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCS1614762.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(20433..21695)
                     /gene="wecC"
                     /locus_tag="OQN13_001369"
     CDS             complement(20433..21695)
                     /gene="wecC"
                     /locus_tag="OQN13_001369"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCS1614763.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(21692..22822)
                     /gene="wecB"
                     /locus_tag="OQN13_001370"
     CDS             complement(21692..22822)
                     /gene="wecB"
                     /locus_tag="OQN13_001370"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCS1614764.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(22878..23924)
                     /gene="wzzE"
                     /locus_tag="OQN13_001371"
     CDS             complement(22878..23924)
                     /gene="wzzE"
                     /locus_tag="OQN13_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCS1614765.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(23936..25039)
                     /gene="wecA"
                     /locus_tag="OQN13_001372"
     CDS             complement(23936..25039)
                     /gene="wecA"
                     /locus_tag="OQN13_001372"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCS1614766.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(25279..26538)
                     /gene="rho"
                     /locus_tag="OQN13_001373"
     CDS             complement(25279..26538)
                     /gene="rho"
                     /locus_tag="OQN13_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCS1614767.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(26623..26724)
                     /gene="rhoL"
                     /locus_tag="OQN13_001374"
     CDS             complement(26623..26724)
                     /gene="rhoL"
                     /locus_tag="OQN13_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCS1614768.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(26865..27194)
                     /gene="trxA"
                     /locus_tag="OQN13_001375"
     CDS             complement(26865..27194)
                     /gene="trxA"
                     /locus_tag="OQN13_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCS1614769.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            27325..28590
                     /gene="rhlB"
                     /locus_tag="OQN13_001376"
     CDS             27325..28590
                     /gene="rhlB"
                     /locus_tag="OQN13_001376"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCS1614770.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            28724..30208
                     /gene="gppA"
                     /locus_tag="OQN13_001377"
     CDS             28724..30208
                     /gene="gppA"
                     /locus_tag="OQN13_001377"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCS1614771.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(30255..32276)
                     /gene="rep"
                     /locus_tag="OQN13_001378"
     CDS             complement(30255..32276)
                     /gene="rep"
                     /locus_tag="OQN13_001378"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCS1614772.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         8230 a         8824 c         8185 g         7393 t
ORIGIN      
        1 tcatcgcagg agaagacgta aaagcaagcg tttagcgact ctttatgaag tctgaaaagt
       61 gaggaaatgc tgcgagtata acgcttactc gtggggattt atgttttgct ggtagtttcg
      121 tgatcggcac ctcgcgccag gatggctcta tgcactatcg caatgtggtt tgtttgttgt
      181 cttttttagt tccgtcagcg tgaggatgct cgctcggtaa acctgatcat aatgtttacc
      241 agaaacaggg aaaggcgtgg tgtaactgcg ccctaatgaa gagacaaatc tttcgctaca
      301 cagagacatc attattccgg tgacggatga atcgtgccgg aacgcagccg cagaggtgca
      361 aactttgaag ccctgacccc atgccgctat actgccgcca tcacttaaaa acaggataca
      421 acaatgaacg acagtgaatt tcatcgcctg gctgatcaac tctggctgac cattgaagaa
      481 cgcctggacg actgggatgg cgacagcgat atcgactgcg aaatcaacgg cggcgtgctg
      541 accattacct ttgaaaacgg cagcaaaatc attatcaacc gccaggaacc gctgcaccag
      601 gtatggctgg caaccaaaca aggcggctac cattttgatc tgaaaggcga tgagtggatt
      661 tgcgatcgca gcggcgaaac cttctgggat ttgctggaac aggcggcgac gcagcaggcg
      721 ggtgaaacag tcagtttccg ctaagattgc atgccggata agcctcgctt tccggcacgt
      781 tcatcacgaa aaatattgct gtaatagcgg cgtatcgtga tcctgattgg caggcggcat
      841 gttaccgata gattttgtgc ggaacggaat cacctgttca cgaccatcaa ccttcacaat
      901 ctgatagaac tgcggcaggt tgaagttgat gaagcttgag ccgtaggtga agcggtcgtg
      961 cgatgacgag tagaagcgac tgacgtcacg caccagttcc tctttgctgc cttcgcagtg
     1021 gtgatacacc tcaacccggt tgctttcgtc aagaatgtag atattaaagc cattctcgtc
     1081 ttgcgtttct tcgaagaaga actggatgat cccttcacta gcaaagccgt ccaccaccgc
     1141 cggtaatttg acgtgattgg tttcaacctg cactgacagg ccgtgcagtt tgttatgcga
     1201 aatagcgcca taaaactcga tggcgttctc cagcttctgt accgatacat tcaggcgttc
     1261 gaagaacaag ccccaggttt gaccagaaac gcgcagcgcc ttgaaacgcc cggtttcctg
     1321 gcgggtgctg gaaaggcgca gttcaataca ctcagaaacc agttgctgca cgcgagtacg
     1381 gattaacccg cgcagatgct ggctataaca gaagacttcc acgctatctg gcggtgcggc
     1441 gtcctgatgc attttgccga gaatagtttt cagggcttcg atcatcgatt gctcgccgtt
     1501 gaagtgcagc gtacgcactt cgttccacga gttgcggtac agcaggtcaa cgctacctac
     1561 caggcaattt tgattctcgc caaagctgaa gacatccagc ttacggaaat caaaatgcac
     1621 cacctgattg cggaacgccg ctgtcgggtc atattccagg ttaacgataa tcgccagatg
     1681 gcggatctca cacgggctgt agagcgcctt cggtgtcggt gcaggtaagc gcagcgggaa
     1741 atggtgcgac acgtcggcga ccatctcctg caacttaggc aaatcgacaa tgccgttacc
     1801 tttaatatac aaacgggtgc gcgaggtcag cagaccgtta aaccatgccc acgccaccag
     1861 tttattcagg taacggttat attccagcgg ctgatggctg atgatcgact caatatttgg
     1921 cgcgcggtta tacagatacc aacctgaacg gttagcccgg cccggcggca cataaataaa
     1981 ggtcagattc ggttccgaga gatcgggtga aatctgcggg tttaccagcg tcactttacc
     2041 tggtaatgct tcaaacgcgg catacagctt acgcgtcagc acgccgatat cctgcggact
     2101 ggcggagacg ctaaggttat tgcgacgcgc aaagcggatc agattacggt agctctgcat
     2161 catcgcgtcg agcaactcgt tgtgcgcctc acgcacctga tcaatcttcc agttagcacg
     2221 gttatcgagc attgccagac gagcttcgtc ccaaccccac tcgctcacta actggctcaa
     2281 cactgcgcga cgccagccta cacaggcgcg ttcacggctg agtttttcgc acacttttaa
     2341 atagaagcag cgacgtacta aatccagacg ggtgaaatct tcaatcgccg tcaggtattc
     2401 agtaacacgc tccagcatca tgcagtatgg atcgaggcca aacgatacaa tctcgccgtc
     2461 gtgcaaacgc tgtttgatat ctttcgccag cagacgtggg ttcgggtatt cccaggaata
     2521 ggcttccagc agcagtgttt tcagcaccgc tttgtatggg gaatcgatac tcttgtagag
     2581 ctgccaaagg ctggcaccaa agtactcttc agcagaaagc gagcttaagc caccgagatc
     2641 cagccattca tttggcgtca gcacgccctg cgcgtaaagc gtcatcacat agtcgtcgta
     2701 atgctcttct tcgtcgcacg gcaccatatt ccacagaata cgcttaccgg cgagacgcac
     2761 ggcggtacga taaaattcgt caagcagcag tatatgctgg gtggagccac aatcttcgcc
     2821 ccccaggctg ccgctttcat tatgacgaaa gcggttttca tcaatcagga agaagctgac
     2881 ttccacaccc agcgaggcgg cccagctttc cagcaggctg catttacgtt gtagtaactg
     2941 gcgctcttcg ctatcgagcc aggattgatg acagacccag atatccaggt cagaggaaca
     3001 actttgccct acggacgagg tgctgcccat ggtgtataca ccagtaattg gaagctcacc
     3061 tttcggtgga tcctgtactg acattccacg atacagttca agctcgttca ggtagtggcg
     3121 ttgagtttca tcaggcgtgt aaaggcaaat gcctttggga acgttaccat caaggtaacc
     3181 cggcattagc ggatggtgat agtgcaacaa tgtcggcagt agactgtaga cctgttggaa
     3241 tgcaggcccc atagcagcaa gcgcgcgatc cacacgcaat tgatttatgg catccagtct
     3301 ctgtttcaga gtctcaatat agaggtacaa gacgtatcgc ctgatttgct acccgtcatg
     3361 actgtgattc cgccaacatc aacggtaaca cgcggcattc gggatatttc gtatgtcaaa
     3421 ggtaaccgtt accacttttc gcgcctggtt tttttagttt cacgacgaaa aaatggtcta
     3481 aaacgtgatc aatttaacac cttgctgatt gaccgtaaag aaagatgcgc tacatacaag
     3541 tgtagcaccg tttattctct gtaaattcct tattacgaca gcgtgaaacg tctgtcagga
     3601 tccactgcca gacctcattt tacggtttgc gcaggcgtct acgtttcacc acaacactga
     3661 catcactctg gcaaggatgt taggatggac cacggatgat aatgacggta acaagcatgt
     3721 tagacaatgt tttaagaatt gccacacgcc aaagcccact tgcactctgg caggcacact
     3781 atgtcaaaga caagttgatg gcgagccatc cgggcctggt cgttgaactg gtaccgatgg
     3841 tgacgcgcgg cgatgtgatt cttgatacgc cgctggcgaa agtaggcgga aaaggcttat
     3901 ttgtaaaaga gctggaagtc gcgctcctcg aaaatcgcgc cgatatcgcc gtacattcaa
     3961 tgaaagatgt gccggttgaa ttcccgcaag gtctgggact ggtcactatt tgtgagcgtg
     4021 aagatcctcg cgatgccttt gtgtccaata actatgacag tctggatgcg ttaccagcag
     4081 gcagtatcgt cgggacgtcc agtttacgtc gccagtgcca actggctgaa cgccgcccgg
     4141 atctgattat ccgctccctg cgcggcaacg tcggcactcg cctgagcaaa ctagataacg
     4201 gcgaatacga tgccatcatt cttgccgtag ccggactaaa acgtttaggt ctggagtccc
     4261 gcattcgcgc cgcgttacca cccgagattt ctcttccggc ggtaggacaa ggtgcagtcg
     4321 gtattgaatg ccgccttgat gatacgcgca ctcgcgagct gcttgccgcg ctgaatcacc
     4381 acgaaactgc actgcgcgtt accgcagaac gcgccatgaa tacccgtctc gaaggcggat
     4441 gtcaggtgcc aattggtagc tacgccgagc ttattgatgg cgaaatctgg ctgcgtgcgc
     4501 tggtcggcgc gccggacggt tcgcagatta ttcgcggtga acgccgcggt gcgccgcaaa
     4561 atgccaaaca aatggggatt tcgctggcag aagagctact gaataacggc gcgcgcgaga
     4621 tcctcgctga agtctataac ggagacgccc cggcatgagt atccttgtca cccgcccgtc
     4681 tcccgctgga gaagagttag tgagccgtct gcgcacactg gggcaggtgg cctggcattt
     4741 tccgctgatt gagttttctc cgggtcaaca attaccgcaa cttgctgatc aactggcagc
     4801 gctgggggag agcgatctgt tgtttgccct ctcgcaacac gcggttgctt ttgcccaatc
     4861 acagctacat cagcaagatc gtaaatggcc ccgactacct aattatttcg ccattggacg
     4921 caccaccgca ctggcactac ataccgtaag cggacagaag attctctacc cgcaggatcg
     4981 ggaaatcagc gaagtcttgc tacaattacc tgaattacaa aatattgcgg gcaaacgtgc
     5041 gctgatatta cgtggcaatg gcggtcgtga gctaattggg gataccctga cggcgcgcgg
     5101 tgctgaggtc actttttgtg aatgttatca acgatgcgca atccattacg atggtgcaga
     5161 agaagcgatg cgctggcaat cccgcgaggt gacgacggtc gttgttacca gcggtgaaat
     5221 gttgcagcaa ctctggtcgc tgatcccaca atggtatcgt gagcactggt tactacactg
     5281 tcgactattg gtcgtcagtg agcgtttggc gaaactcgcc cgggaactgg gctggcaaga
     5341 cattaaggtc gccgataacg ctgccaacga tgcgctttta cgggcattac aataactctc
     5401 ataacaggaa gccataatga cggaacaaga aaaaacctcc gccgtggttg aagagactag
     5461 ggaggccgtg gacaccacgt cacaacctgt cgcaacagaa aaaaagagta agaacaatac
     5521 cgcattgatt ctcagcgcgg cggctatcgc tattgctctg gcggcgggcg tcggtttgta
     5581 tggctggggt aaacaacagg ccgtcaatca gaccgccacc agcgatgccc tggctaacca
     5641 actgacggca ttgcaaaaag cccaggagag ccaaaaagcc gaactggaag gcattattaa
     5701 gcaacaggct acgcagcttg aacaggcgaa tcgtcagcaa gaaacgctgg caaaacagct
     5761 ggatgaagtc cagcaaaagg tcgccaccat ttccggcagc gatgctaaaa cctggctgct
     5821 ggcccaggcc gattttctgg tgaaactcgc cggacggaag ctgtggagcg atcaggacgt
     5881 cacgaccgct gcagcgttgc tgaaaagtgc agacgccagc ctggcggata tgaatgaccc
     5941 gagtctgatt accgttcgtc gggcaattac cgatgatatc gccagccttt ctgcagtatc
     6001 gcaggtggat tatgacggca tcatccttaa gcttaatcag ctttcaaatc aggtagataa
     6061 cctgcgtctg gccgataatg acagcgatgg ttcgccgatg gattcagacg gtgaagagct
     6121 ttccagttcc atcagcgaat ggcgtatcaa tctgcaaaaa agctggcaga actttatgga
     6181 caacttcatt acgattcgcc gtcgtgatga caccgccgta ccgctgttag cgccaaatca
     6241 ggatatctat ctgcgcgaaa atattcgctc tcgcctgctg gtcgcagcac aagctgtacc
     6301 gcgtcatcag gaagagactt atcgccaggc gctggagaac gtctccacct gggtacgtgc
     6361 ttactacgat actgatgatg ccaccaccaa agcgttcctc gacgaggtgg accagttaag
     6421 ccagcaaaat atctcgatgg atcttccgga aaccctgcaa agccaggcga tgctggaaaa
     6481 actgatgcag acccgcgtgc gtaacctgct ggcacaaccg gcagcgggga caacggaagc
     6541 taaacctgca cctgcacctg cacctgcacc tgcacctgca cctgcacctg cacctgcacc
     6601 tgcacctgca cctgcaccgc aaggtgatac tccggcagcc gcgccgcaag gagaataacc
     6661 atgctaaaag tgttattact ctttgttttg ctgattgcgg ggatagtggt tggcccgatg
     6721 attgccggtc atcagggtta cgtgctgatc cagactgaca actacaatat cgaaaccagc
     6781 gtcacgggcc tggcgattat tttgattctg gcgatggtag tgctgtttgc cattgagtgg
     6841 ctactgcggc ggatcttccg cacaggcgca cacacccgtg ggtggtttgt cggacgtaag
     6901 cgtcgccgtg cccgtaagca gaccgaacag gcgctgctga aactggcgga aggcgattat
     6961 cagcaagttg aaaagctgat ggcgaaaaat gccgatcacg cggaacaacc ggtggtgaac
     7021 tatctactgg ctgccgaagc cgcgcaacaa cgtggtgatg aagcacgcgc caaccaacat
     7081 ctggaacgcg cagcagagct ggccggcaat gacaccattc cggtagaaat cacccgtgta
     7141 cgtctgcaac tggcccgtaa tgaaaaccat gctgcacgcc acggcgtgga taagctgctg
     7201 gaagttacgc cacgccatcc ggaagtatta cgtctggcgg aacaggcgta tatccgcaca
     7261 ggtgcatgga gttctctgct ggatatcatc ccatcaatgg cgaaagccca tgttggtgat
     7321 gaagaacatc gtgcaatgct ggaacaacag gcatggattg gcctgatgga tcaggcgcgt
     7381 gccgataacg gtagtgaagg tttgcgtaac tggtggaaaa accaaagccg gaaaacgcgt
     7441 catcaggtag cgttgcaggt ggcaatggcg gaacatctta ttgaatgtga cgatcatgat
     7501 actgcccagc aaattatcat cgatggcctg aaacgccagt acgacgatcg cctactgctg
     7561 ccgattcctc gactgaaaac caacaatccg gaacagctgg aaaaagtgct gcgccagcag
     7621 atcaaaaacg tcggcgatcg cccgctgttg tggagcacac tgggccagtc actgatgaag
     7681 cacggagaat ggcaggaagc atcgctcgcc ttccgcgcag cgctgaaaca acgtccggac
     7741 gcctacgatt acgcatggct tgccgacgcg ctggacagac tgcacaagcc ggaagaagct
     7801 gcagcgatgc gtcgcgacgg tttgatgtta acgttgcaga acaacccacc acagtagttc
     7861 cttctcaccc ggaggcaagc acctccggga cctttctgat gcataaaaaa acgcctgctc
     7921 ttattacgga gcaggcgtta aaacaggtct gtatgacaac agtgggtgct tcactcaacg
     7981 ttgtgtccat ggtgtctgat gaggcataag cgacatctgt cagtggacga taagcaccgt
     8041 aaacggctct gcgtcattcc ggagtttatg aggcactaag gcgaacataa gagatggaat
     8101 gagcatctac ccgtttatta tgccacagag aatcgggaaa taacatccct taacacttgt
     8161 tatgagataa ttctgtaatc ctctttgctt cctgagtgaa tatttaacct gagcttgatc
     8221 ctacacatac ttattatgaa tgataaaatt cattcaatta ataacacata tactaattgc
     8281 cgttaaaact aaaaaagcat taataatcaa cgcaatataa taaacccgcc ttacatatca
     8341 actgcgccag aggtaggatt gaaaacgctc tcctgatttt ccaattcatt ttctggataa
     8401 ataaataatt tatttttgtc actattattt atgtaatcat cctgtcaggg agagggatct
     8461 caattatcaa tgcttaatta cgtcatcatt ttgatgacat gaaaccggag cattttgcat
     8521 ggaattttcg ttttcaccca aacttcttgt tgttgctgtc gccgccgctc ttcctctcat
     8581 ggccagcgca gcagataccc cgtcaactgc caccgcacgc aaaggctttg ccggatacga
     8641 tcacccaaac cagtatccac agaacgctgc aatttattga atttgcacga tcatgtaggc
     8701 cggataaggc gtttacgccg catccggcaa tcaaccgcag gcggccacaa aatggccgct
     8761 tttttactta ttcaccacca gcaaatgtgc ctgcataatg tcactggccg ggcgaccgtg
     8821 cgccagcaaa tcagccattg ctttaagata tggcggtaga tggcggaaat aacgctgata
     8881 cccggcacac aaataattca gccccggttt gccgctggca tcgagcatga agcggtgttt
     8941 cgggcagcct ccccagcacg cttttaacac gttacaactg cgacactgcg ccggtaattg
     9001 cttaaattta tcttcaccaa acacctgctg ttgcggggaa tcgatcattt ctgcaattgt
     9061 ttgctgatgc atattcccca gccgatattg cggataaaca tagtgatcgc aggcgtaaac
     9121 gtcgccgttg tgctcaacaa tcaccgagcg cccacaggtt ggctgatgat ggcaaaccgc
     9181 acccggcgca ccgacaaaat tggcaaacgc ccattcgata ttcatcacga aaatcttgcc
     9241 gacgtcgcgt ttgatccagt ggtcgaatat cgccaccaga aattcaccaa attcatcggg
     9301 gcgcaccgac cattccgtca gttccccctg aatatcacca ggcgcatgca atttcagtcc
     9361 atcgcgggca gctgtttcat cagccagacg ctcgaccacc ggaataaact ggatgaattc
     9421 gactcccgca tcgcacaaaa aatcataaac ctgcaacggt tgctgcgcgc tggtgcggtt
     9481 gacgcagacc agcacgttat agtcgacatg atgtttttgc agcagcgtca gggcacgcat
     9541 caccagctta tgcgttggtc tgccaccttt ggtcacgcga tattgattgt ggatctcagc
     9601 cggaccgtcc agcgataacc caacaagaaa atgattttct gccagaaatg cacaccattt
     9661 atcatcgagc agcacgccgt tagtctggaa gctgttactt atcttcctgc cagcaccgta
     9721 tttcgcctgt aactttacgg cacggcggta aaaatccagc ccgagtaacg ttggttcgcc
     9781 gccctgccag gtaaaagcca cttcgttttg cggttcgctg gcagcgatat agtgacggac
     9841 atacgcttcc agcgtgtcat cgtccatatg cgtgactggc ttttcgcggt aaagggattg
     9901 tttttcgaga taaaaacagt agtcacagtt cagattacaa tcggaaccac tcggtttcgc
     9961 catcacatga aaagcacgcg ttggaacctg ttgcagcatg gtcactcctt gcctgaagtc
    10021 atccttcaat acgttaaggg cgaaatcata ccgtggggca atgagttatt acgggcgttt
    10081 attgcgaagg gagggtgacg aaatgcacag aaaacaaaaa accccgccga agcggggttc
    10141 aaaattggtc ggcgagagag gattcgaacc tccgacccac tggtcccaaa ccagttgcgc
    10201 taccaagctg cgctactcgc cgaaatactg ctttttgaat ttttagttca attctttaaa
    10261 gtcgtggtgc gagggggggg acttgaaccc ccacgtccgt aaggacacta acacctgaag
    10321 ctagcgcgtc taccaattcc gccaccttcg cattgtcaca acttctaata atggggtggc
    10381 taatgggatt cgaacccacg acaactggaa tcacaatcca gggctctacc aactgagcta
    10441 tagccaccac tacaaatctt gttacgcggt attactacca ccgcagctca agcgccggga
    10501 ctaaatggcg cgcccgacag gattcgaacc tgagacctct gcctccggag ggcagcgctc
    10561 tatccagctg agctacgggc gcttagcgcc gttgcggggg gataatacgg acttcccacc
    10621 cctctgtcta gtcccttttt aaataaattg cgcgtttggt tacggtttgt gcgttttgct
    10681 gcttattcct ccagtttatg cgctttcccg tggcgattaa ggccaaaaac tttataaatc
    10741 gccgtcaccg ccagcataaa gatgatgcca acaaacagcg acatacgcgt atcttcatta
    10801 aagtacatgc cgatcaaaac gcaaatcagg aatgccattg ttacgtagtt ggcccacggg
    10861 aacaggattg agcggaacgg atggctggca atcgccgctt tatgtgcacg ccgaaaacgc
    10921 agctggctta tcaatatcac aaaccatggc accatccccg gaagcacgct ggcactgtag
    10981 acgtagacaa acacacgctg cggattggga atgatgtagt tcaggcatga gccaattagc
    11041 agaatagcaa tagatactgc cacacccgca actggtacgc cgtgacggga aactttcgcc
    11101 atcgccgccg gtaactgacg gtttttcgcc agtgcgtaga gcatacgtcc gcaactgtac
    11161 atgccgctgt tacagccaga gagcgcagcc gtcagcacca caaagttgat aatgcccgcc
    11221 gctgcggtaa taccgatttt ggcaaaagtc agtacgaacg ggctgccgtt gctgccaatt
    11281 tcattccacg ggaagatggt gacgataacg aaaatcgcgc ctacgtagaa aatcaggatc
    11341 cgccacagca ctttgcctac agcactgcgc agcgtcacct gcggattctt cgcttcaccg
    11401 gcagtaatgc caatcagctc cacgccctgg taggacgcca ccacaataca cagcgcggtc
    11461 aggaaccctt tccagccacc cgcaaagaaa ccgccatgct ctgtgagatt gctaaaacca
    11521 atcgactgcc cgccattgcc aaagccaaag aaaatcacgc ccaggccaat gacaatcatc
    11581 acgataatcg tggtgacttt gatcatcgcg aaccagaact cgatttcgcc gtacaaccgc
    11641 accgccgcca gattcgccaa cgccaccagc gccaccgcga tcaatgcagg tatccactgc
    11701 gccatctccg ggaaccagaa ctggacataa acgccaatgg cggtgatttc tgagatcccc
    11761 accgccatcc acataaacca gtaagaccag gcggtgagat aaccaaaaaa cgggctcata
    11821 taacgatgcg cataaacggc gaacgaaccg gtaacaggtt caaggaacaa catttcgccc
    11881 attgaacgca tgatgaaaaa gacgaacagc ccggcgatga tataggccaa caatacggat
    11941 ggcccggccc atttcagggt actggcggcc cccataaaca ggccgacgcc aatagtgccc
    12001 cccagggcga tgagttcgat atgtcgagct tccagcccac gctgtagctc tggtttgtta
    12061 tctgccataa atcctcgtgt tgtgtttgca tgctttccgg tgttaccggt tatcgttatg
    12121 ggtacatcga gtgttgcgaa tgttttcgta attcaggaga aatggcaaat aaagcattaa
    12181 aaatttgaat gctttgtgta ataaaaaagc agacaggcga cggagtgacc actccgtcgc
    12241 tttacaaaga gaggaaaatc agaggttgcc ggtgtagtgc cagcgtaaat aacgcagcaa
    12301 acgaagctga cgcttaatgc ggctcggctg cgaaagcagg cggtagagcc actccagtcc
    12361 cagcgtttgc cagattttcg gtgcgcgttt tacgtgaccg gtgaaaacat cgtaagtccc
    12421 gccaacgccc atatacagcg catctggatg caccagacgg cagtcgcgca tgaagatctc
    12481 ctgctttggc gatcccatcg caacggtgac gatttgcgct ccgctggcat gaatgcgttc
    12541 aaacagcgcc tgacgctgct cgggtttaaa ataaccatcc tgactgccaa cgatattcac
    12601 attccattgg ttgcgcagtt tagcttcagt ttgcgccagc acttcaggtt taccgcccac
    12661 aagaaatacc ggcgtccctt ctttgcctgc gcgcgccatc agctcttccc agagatcggc
    12721 accggcaacg cgggaaacct gcgcctgcgg gtacttttta cgtactgaac gtacaacgct
    12781 gatgccatcc gcatatttaa attcggcagc gttaattaac tccctgacct cggcgttatc
    12841 ttcaatggtc agcatttttt cagcattaat ggcaaccagc gttccctgct taagctgccc
    12901 gtcagcaaac agataatcga gggcgtgctg catatcacgc caaccaatca actgtaagcc
    12961 acgcagcgta taggtcggtg ccgtggtgtt gttattcatt gttatccttc aacctgcgtc
    13021 cggagcgatg attttgtacg tttatgaatg agtccggcgc tttcaaaaag ccagtacagc
    13081 agttttgcga tcatcagaca tgcgccgaag accacgataa aaaagactac gcgtgagaca
    13141 aacgaatcca gcccttcacg cgccagcacg atcatattga agatcgcccc aaagcagaaa
    13201 ctgtgcaata tcgcagcctt atagcgatta ggctcgcggt tgcccagctc atacagccag
    13261 tcgaaccatt tgatgatcag accaaccacg atcgccccga gcgggatgaa caacgcgccg
    13321 cccatcacca ccagtgagcc tataagcgta ggcgagatcg ccagtccgga gtggttattc
    13381 agcacttccc aggtaaagta gttggctgag ttcagcacca tactcgggcg acccggccac
    13441 agccaggaag ggataaagac atagaaatcg cggacaattg gagccaggcc ctggaagtcg
    13501 atgttgtcgt agttctgcaa cagcaacgcc agattctccc acggggagaa ggtgtcgcga
    13561 gtgagataga gaaacgtata gaacgcttca tcgccgctca cattcattcc atagcgtttt
    13621 agcgccagcc agaacatgcc aacaatcccc agcacgcccg ccgccgccag catccacaac
    13681 gaaatccagc cgcgaataat gccaataaac aggaagatag cgaatgcgat gatgatattg
    13741 gcgcgagtgc cgccgacaat catataagtc agcaagccaa aggcgaccgt gctgacgagg
    13801 aaaaacagcc acgctttgct gtcctggcgc agaaagtaga ccaccagcat cgccgggatg
    13861 aaaaagtaaa agaagcgttt taacgccacg ccggagactt cactggagaa gatctgactg
    13921 taggagttaa gccggaacag caaaaagccg ttgtgcatga agaagatgcc gacgcttacc
    13981 agcgcgatac ccatcaggat cacccacgta agattggtct ccacgcggtt catggtaaac
    14041 agcggacggc gcggtacatc agcaacgcgt ttgcgtaggc gggttttgta ggtgacatag
    14101 taaaccgcgt agaagcagcc cgcagaaagc aacgcctgca acaagatttc tggcggcgcg
    14161 acaccaacat caaagcgaaa taccagcacg ctggtcagcg ggaagccgaa gaaaaaggtg
    14221 agcaaaaaca gcaatgaaaa gaagacattg aagttaaagc gcacacggcg aaactcaaac
    14281 caggtcagcg tggcaataaa cagcgtgcag agcagccaga ccacaaacag gccactgaat
    14341 tgcagcagac tcatgcgacc tccccggcgg caatagccaa cgcccgctgc cagccttgta
    14401 gatagttagg gctaaagaag gcaatggtgt ttttatccac cgacgccaac tggcgctgcg
    14461 cttcacgcac aatatcctcg ttgagatcgt cggtagtaaa cagcaccggc aaatgctgtt
    14521 ccgtcatatc ctgccagaac ggattttccc ggttaagcac acaaggaatg cccgcctgaa
    14581 tcagtaagca aagcgtacca atgccctgct ggcgggcaaa aataaagtaa ccaagatcgc
    14641 actgacgaag tagcgccaga taggcgtcaa attccagttt ttcgctcaaa atttgcagat
    14701 tttcttcgct gaataactcc agccccgcct gacgaacttc ctcaatgtac gcttcgttat
    14761 taggcggata tcccatcggc accaccactt ttaccgtatc gccaaattgc tgatgaacgg
    14821 cgcgcaaggc agcaatatgg tcattgctgc ggtcaccgga gttccccacc agaatggtca
    14881 ttttcccttc acgttgccga tcattcgcca tcgtattgag agaagggtcc atccgcgtcg
    14941 ggaagtacag cagttcgccc cgcacctttg gatgcgtttt ggcaaaaaag ctcaaatcac
    15001 cgcgggtggc aaatacacag ccgactcgct tttgcgccag gcgacgtagc gggtaaaaaa
    15061 gcttatatct caagccactg gaaagctcat acaggtctgc cccccagata tgccagaaaa
    15121 actggctggg cttaatgcca ccactcagca gagccagcca cagtgtggga ttgaactgac
    15181 cgtggaaaaa aaatcgctgc tgacggttag cttttgcttt cgcgatgacc gcttccgcca
    15241 gcgatttttt cccagggaaa aattgcacag aaagcgccgg acagctatca cttaagccgt
    15301 cgtccttgcc aacaaccata aactcgcgcg cgtgctcgct cgtcgcggcc agcgcgtcat
    15361 tgaaaaaccg caaaacggtt cggttatggt gagggatatc cgatcccagt acgtgaatca
    15421 gtacagtcat gcccgcctac gccagagtaa aaacacgcca caacaaagag aaaaatagac
    15481 gatataagtt gccatatatg cctgcgccgc gcccagtgca ccatgcgcag ggattagcca
    15541 gtgggcaaat accatcaata aagtgaactg gctgacttcc gccagaatat aaaaccgcag
    15601 tgacgctttg gcgatcacca gataaccaaa gacataagcg cccactttta acacatcacc
    15661 cactaactgc caggcaaaga gatcgcgcat agcggtaaat ttattcgaca acagcaacca
    15721 gatagcaaaa tcacgcagca accagacggt aaaactcgcc gccgccactg ccggtaagac
    15781 gaatttcagc gatttaacca cttcccgggt gatatcgcgc ttttccgtta gccgcgacaa
    15841 cgtgggcagc aaatatacgc tgaacgatgc cgtaataaat tgcaggtagg catcggaaat
    15901 actgctcacc ccttgccaga tccccacttc atcccagcta tactgcgccg ccagcagttt
    15961 acgcatcatg atgtaagcaa caggcaaggt caccgacgta atcaacgcca tgagcgtaaa
    16021 tttgctcaac tgccctgcca gaccgttatc ccagctgggt ttcagatagc ttaacgggat
    16081 gacaccacgt ttgatcaaca tgatggcggc aggaatcacc accagcgcgg gaatcagcgc
    16141 cagacccagc aacgcccctt cataaccgcc caaacggtaa ctgacgtagt acgcgagaac
    16201 gccaatcaag ctgccgacaa tcagcgataa cgcattacct gcggcatcgc gaaagccttt
    16261 catcagcgcc agtaacaggt tgccccaggc gatccccatt tgcaccagcg ccactaaacg
    16321 caccagcccc tgatagtcgg tattaccaaa cagtccctgg ctgattggcg cagctgccag
    16381 cacaaaaacc agcgccatca gcgtagagaa accaagtacc atcgctgatg aagtgccgac
    16441 cacgcggcgc agctgttgcg gattatcatg gtactgggca acgtatttgg ttacaccgtt
    16501 aaagatgcca gctccggcaa gcacgccgag cacggtaatc aactggcgga aatttgccgc
    16561 cagcccaagc cccgccggac caaatgacac agccagcaac ttaccgacca gtaacccggc
    16621 accaatcttg accagtgtac tggccgccgt ccacaaggac gcttttgcca acgacatatc
    16681 aggagaagta gttcaacaaa gtcgcaatta ccgtacgctg attgacgggc gacaggttgt
    16741 agaacagcgg caggcgcagc aggcgctcgc tctctttggt ggtgtagcga tcttcaccgt
    16801 ggaactcacc aaagcgttcc cccgcagggc aaccgtgcag cggaatgtaa tggaacaccg
    16861 ccatgatttc cgcttctttc agaaagttaa tcaacgcgct ccggtcatca atatcccgca
    16921 gtttaatgta gaacatatgc gcgttctgca cgcagccatc gggaatcgac ggcagctcga
    16981 tacgcccggc tttcgccaga ggcgctaacg catcgtagta gttttgccac agcgccagac
    17041 gttgctggtt gatacgatcc gctgcttcca gttgcgccca caggtacgca gcttgcagat
    17101 cggacatcaa atagctggag ccaatatcgc gccaggtata tttatcgacc tgaccacgga
    17161 agaactggct gcggttagtg cccttttcac ggatgatctc ggctcgttcg attaacgctt
    17221 tatcgttaat cagcgtcgcg ccgccttcac cacccgccgt atagtttttg gtttcatgga
    17281 agctaaagca gccaatatga ccaatggttc ccagtgcacg ccctttgtaa gtggacatca
    17341 cgccctgagc ggcatcttct accacaaaca gattatgctt tttcgccaac gccataatgg
    17401 tgtccatttc gcaggccaca cccgcgtaat ggaccggcac gataacgcgc gttttgtcgg
    17461 tgatcgccgc ttcaatcagc gtttcgtcga tgttcatggt gtccgggcga acatccacaa
    17521 aaacgatttt tgcgccacgc agcacaaagg cattggcggt ggagacaaag gtgtagctcg
    17581 gcatgatcac ttcatcgcca ggctggatat cgagcagcag cgccgccatc tccagcgaag
    17641 cggtgcagga cggcgtcagt aacactttgg cgctgccaaa acgttgctcc agccactgct
    17701 ggcagcgacg ggtaaaaccg ccatcgccac acagtttgcc gctacccatt gccgactgca
    17761 tatagtcgag ttcggttccc accaccggcg gtgcgttaaa tggaatcatg tgatcacctg
    17821 tataaccagt acgcggtgct ttctacattc gcaccacttt gtatgtatcg tttaagcgcg
    17881 gcggtgttgc ccatttgggt cgccacccgc aaagttgttt taccgcgagc atacgcccag
    17941 tttagcgccg tttgcatcag ctcagcacct gcaccgcgtc cagccagcag gccaattcgc
    18001 gcatctgtcg cattgagttc ccgtaaagag acataaccgc gaataccgcc ggacgccgca
    18061 cgtaaaatca gacattgatg atcaaaggtg ccgcgcacgg cattttcaat ccactgtgca
    18121 taaaagcgtc cgctggcgtc aggcgcatac cacggcgcac gaaaacggct ttgcgcaaat
    18181 gcggcgctgg ctaactgacg taatgcggga atatcggtct cttgtgccac tacagcacca
    18241 ctatcactga cattgttcac gggtagtgcc aaatcaactt caccttctac cagggagaat
    18301 cccagctgtt gcagggcatc cagttcaccc gtatttgatg ccgcaatttt ggcctgcacc
    18361 cgtgaccacg gcgctaacac gtctggcgtc aggagctgtg cttcagacgt aatgcgcacg
    18421 atggcgctgt taacaccaaa gaaggcgttt tcccaggtta gtggctcaat actggcgcgg
    18481 acgggcacga agtaactcca gcagatattg gccgtagcca gttttcgcta atgaactggc
    18541 agcacgcttc acaccctcgt catcgagcca gccgttacgc caggcgatct cttccaggca
    18601 ggcaatctta aagccctggc gtttttctac tgtctgcaca aaggtactgg cttcaatcag
    18661 gctgtcgtga gtgccagtgt ccagccaggc aaatccgcgc ccgagcagtt caacggtcag
    18721 gttgcccgcc tcgaggtaca tctggttgat ggaggtaatc tccagttcac cacgctccga
    18781 cggcttcacc tgctttgcgt actccacgac tttactgtcg tagaaataaa gcccggtcac
    18841 cgcccagttt gacttcggct gttttggctt ttcttccagc gagatagcgc ggaaattgtc
    18901 gtcaaactcc accacgccaa agcgttccgg gtccatcacc tgatagccaa ataccgtcgc
    18961 cccttccgtg cgcgccgcaa catgacgcag cttcggactg aagccctgac cgaagaagat
    19021 gttatcgccc agcaccagac aagaaggttc accgttgagg aaggtttcac cgatgataaa
    19081 ggcctgcgcc agaccgtccg ggctgggctg ttcggcgtat tccagctgga taccgaactc
    19141 actaccatcg cccagcaggc gctggaaata acctttatct tccggcgtag tgatgatgag
    19201 aatttcgcgg atatcggcca acatcagcac cgacagcgga tagtaaatca ttggcttatc
    19261 gtaaatcggc aacagttgct tcgatacgcc gcgcgtaatc ggatgcaatc gggtgccgga
    19321 accgcccgcc aggataatac ctttcatgtt tgcctccgct gaaaattagc ctttcagacc
    19381 taaacgctcg ccctgatagc tgccgtcctg cacctgcttc caccagcttt cattagccag
    19441 ataccactgc accgttttac gcattccact ttcaaaggtt tcctgtggca gccagccaag
    19501 ttcacgggca attttcgaag catcaatggc ataacgcaga tcatgccccg gacggtcagc
    19561 gacaaaggtg atcaagtcac gatagtgcac cacgccgtgc ggcttgttcg gagccagttc
    19621 ttccagcagc tcgcaaatgg tttccacaac atcgagattc ttacgctcgt tgtgaccacc
    19681 aatattatag gtttcaccga ctttcccggt ggtcgccacg caatacagcg cgcgggcgtg
    19741 atcttccaca tacagccagt cacggatttg ctgcccgttg ccatataccg gcagcggttt
    19801 acccgccagc gcgttgagga tcatcagcgg gatcagtttt tccggaaagt ggtaaggacc
    19861 gtagttattc gagcagttgg tgataagcgt cggcagaccg taggtccgca gccaggcgcg
    19921 caccagatgg tcgctgctgg cttttgacgc ggaataaggg ctgctcggcg catacggcgt
    19981 ggtttcggtg aagaaatcat ccgtcgagtg caggtcacca tatacttcgt cggtggagat
    20041 atgatgaaaa cggaacgctg attttttatc ttccgtcagc gtattccagt aagcccgcgc
    20101 cgcttcaagc aatgtataag tcccgacaat gttggtttca ataaacgctg ccgggccgtc
    20161 aatagaacgg tcaacatggc tttcggctgc cagatgcatg acacagtctg gctgatgctc
    20221 agtgaatacg cgtgccagtt ctgcccgatc gcagatatca actttctcaa aggcaaagcg
    20281 ctcgctttgc gcgaccggtg ccagcgacat caggtttccg gcgtaggtca gcttatcgac
    20341 cactaccacc gcgtcgctcg tttcgttgat gatataacgc accagcgccg agccaataaa
    20401 cccggcacca cctgttatca gaatttttct catcagcgcc agactccttt ggcatcgacg
    20461 acatactgct gatggacatt gtcgccattg ataactttaa actgactatg atcgaccagc
    20521 atcaccagca cgtctgccgt tgccagcgcc tcgtcaagct gcgccagagt acaaagcccg
    20581 gtcagttttt tcggcaactg gtggatgtta ggctcaacaa ccagagtttc gccgctatgc
    20641 cactgggcga tcagttcagc gatttccatc gccgggcttt cgcgcaggtc atcaatattc
    20701 ggtttaaacg ccagaccaaa gcaggcgatt ttcagttcac tggcgcgttt atcggtagcc
    20761 gccaggcaat cagccaccgc cgctttcacc tgatcgataa cccagaacgg tttgtgatcg
    20821 ttcacttcgc gcgcggtacg gataagccgc gcctgctggg ggttctgtgc cacgataaac
    20881 cacggatcaa cagcaatgca gtgaccgccc acgccagggc caggctgaag aatattaacg
    20941 cgagggtgac gattcgccag gcgaatcagt tcccagacgt taatcccctg atcggcacaa
    21001 atcagcgaca attcattagc aaaagcgata ttcacatcgc ggaagctgtt ttcggtgagc
    21061 ttacacattt ccgccgtccg cgagttagtg acgacacact caccttcgag gaaaattttg
    21121 tacagttcgc tggcgcgggt cgaacaaacc ggcgtcatac caccaatcac gcgatcgttt
    21181 ttaatcagct cgaccataac ctggcccggt aacacgcgtt ccgggcagta agcaatgttg
    21241 acgtccgcct gctcgcccac ctgctgtggg aaagtgagat ccggacgcat ctctgctaac
    21301 cattctgcca tcttctcggt tgaccccacc ggcgaggtgg attcaaggat caccagcgcg
    21361 ccttttttca gcactggcgc aatggagcga gcagccgatt caacgtaggt catatctggc
    21421 tcatgatcgc ctttaaacgg cgtgggtaca gcaatcagcc aggcatccgc ttcaactggc
    21481 gtcgtgctcg ctcgtaaaaa accgccttct acggcagttt ttactacgct cgccaaatca
    21541 ggctcgacga tatggatttc gccacgattg atggtatcaa ccgcgtgttg gttgatatcg
    21601 acaccaatca cctgtttttg ccgtgaggca aacgctgctg ctgtaggcag cccgatgtaa
    21661 cccagtccga taacagaaat ggtcgcaaaa ctcatagtga tatccgatta ttttttaacg
    21721 cttccagaat gcgagagcat gcctgaccat caccatacgg gttatgggcg cggctcatag
    21781 tttgatattc gttttcgtct tttaaaagac gcgtcacttc ctcgacaatt cgctgcttat
    21841 ccgtgcctac cagacgcacc gtacccgccg tcaccgcttc cggacgctca gtggtatcgc
    21901 gcatcaccag caccggtttc cccagcgaag gcgcttcttc ctgaatgccg cctgagtcgg
    21961 tcaaaatcag ccaggcgtgg ttcatcagcc agacaaacgg taaatactcc tggggatcga
    22021 tcagaatgac atttttcaca tgccccagaa tgcgattgac cggctctctg acgttcgggt
    22081 tgagatgcac cggatagaca atctggatgt cctggtgcgt ggtggcaatg tctgccaacg
    22141 cgtggcagat ttcttcaaag ccacgaccga aactttcacg cctgtgaccg gtcaccagaa
    22201 tcatcttttt atcggggtcg ataaacgggt aatttgccgc cagttctgaa cgcagcgtat
    22261 cgctgctcat cacctggtca cgcacccata acagtgcatc aataactgta ttaccagtaa
    22321 tgaagattcg gctatccgca acgttttcac gcagcaagtt ttgccgggaa gtttcggttg
    22381 gagagaagtg atacatcgcc agatgcccgg tcaatgtacg gttagcctct tccggccacg
    22441 gcgaataaag atcgcccgtg cgcagaccag cctcaacgtg accaacagga atacgctgat
    22501 aaaacgccgc caggctggtt gccagcgtcg tcgttgtgtc gccgtgaacc agcacgacgt
    22561 ctggtttgaa ctcggcaaga ataggtttta gcccttccag aatccgacag gttatctctg
    22621 tcaggccctg tcctggctgc attatgttga gatcgtagtc aggtacaatg gaaaagagtt
    22681 tcagcacctg atcgagcatc tcccgatgct gcgcagtgac gcaaacttta gcctcaaaaa
    22741 aaggatcttt tgccaacgca tgcaccaacg gcgccatctt gatggcttcc gggcgcgtac
    22801 caaatacagt cagtactttc acatcgattc tcttcgaata agcggcgagc gcctttgcgc
    22861 tcaccgcagc agtgttgcta tttcgagcaa cggcgggtta atgcgacacc agccccgatc
    22921 agccccccga caatgcccca cataatcatc aggaaggcac gacgtgggct atcgcgtttt
    22981 accggttctt ccggcgtacg caaatagcga taggtctgaa aacgcggatc cagggttgga
    23041 ccaacattca gggtgtttaa catggcccga ttctgatcat agtcgagatc aaaggccgga
    23101 ccgacggcct gtaaattttc cagtcgagcc tggagcattg gacgcccaag caggaacatt
    23161 tctgaatcag gtaattcctc ggcaggcaca tctgtcgcac tgcgcgaaat attatgctgc
    23221 tcagcaattt tcagcgcctg ttcaatactg ttcatccggc ggtcgtagat ggctttcgcc
    23281 acctcttcct gacgcttcac ctgtgctttc atctggatgg tacgcgccgc ccatgcgcct
    23341 ttcagctcat cattcagatg gctggctgca cgctggctgg caaaagcaac atactgacgt
    23401 aacaggttat tagcgtcagg cgcggtttca gcaataagct tcacgctgtc attgaccgcg
    23461 cgggtaaagt ctccggggat aaactggatg ttgttaatca tttcatccag caacgccgca
    23521 tcggctttgc tgttgcccac catccgctgt ttgtaatagt cggtttgcag ccagaactct
    23581 ctgcgggtat cccacgaggc cagttgcata acaaactctt tataggcttc gtccatgacc
    23641 gatggttggt cggcagaagc catgtttgaa cggacatcca ggttacgcaa aaattgctgc
    23701 tgcgagtaat atccccccag catattcacc gttggacgat cggtaatcgc cgtcgagctc
    23761 cactcctgac gagcaaaaaa agtatacgcc agcgcgatta acgcaaatgc cagccccatg
    23821 ccaataatcc atagcttccc agcccacaag gtacgaaaca acccacgaat atccagttca
    23881 ttttcagcgt cttcggccgg tttcccaggc attggttgtg tcatcacatc ctcatttatt
    23941 tggttaaatt ggggctgcca ccacgatttc tacgcagtct gcgttttacg cgcttaataa
    24001 agcgagcaac tttccaggca cgcttaatgc aatatccata gaggaagaat gctagcaaaa
    24061 agagcaccag catgacccac tccgggacaa aatgagaata ttctgccagc acgccaatgg
    24121 aagcgagcag tgccgcagca agggtaatca gcacaaacgc ctgacgggaa gtaaacccgg
    24181 cacgcatgat caaatggtga atatgctgac ggtcaggaga gaatgggctc atgcctttac
    24241 gcaggcgacg gtacataatc gccaccatat ccattagcgg aatggcgatt atccacaaag
    24301 cggtaaccgg gctgatggga tgggttttgc cctgggtcgt ttcgagcagg atccagataa
    24361 cggtaaaacc aatcagcgta ctgcccgcat cacccataaa gactttgtag cggcgaccca
    24421 ggataccaag gttaagcatg atgtatggca ggatggcggc gatcatcgca aagcaccaga
    24481 ttgcgaggct ggtttgcccg tcgaaccaca aaatcatacc gattgctgca aacgagacgc
    24541 aggacaaccc gcccagcaag ccatcaatgc catcaaccat gttgaacgca ttaatggccg
    24601 cccagacggc aaatagcgtc aggaagtaac caaacggtcc gagcaccatc tcccaggagc
    24661 caaagatata acccaggcta ctgagataaa gtttgccgaa caccatcata acaatgccaa
    24721 cagcggcctg tatggtggca cggattttta cgctgatatc aaaacggtca tccagcgcgc
    24781 caatgaaaac aagcacaccg gcacaagcga gatagagaga tgcatgcgga atatagtaat
    24841 cgacaattcc gaacgtgaag caaatccctg cgtaaaccga aatcccccca acgagaggta
    24901 tcaatccctg gtgacgtttg cggaagtttg gtttatccac taaaccgact ttttttgcca
    24961 ccttacgggc aaaaaacaga aacagtgtcg tgaataaaaa aatactgatg agatcagtac
    25021 tcactgtcag taaattcaca atgcatgctc tcagagaaaa ttattagcag aagtataacc
    25081 acgaagacct ttattcagaa gggaaagtcc gttccgaacc caccagtcct gtgcaaaaat
    25141 taaccatttg atatgcaaat tgcataacac gccagcaaat tccaggaatt ttcctaacat
    25201 cgccagcgcg gcattaagat tacagatata aaagcaaaac gccacgtaaa cacgtggcgt
    25261 ttttggcata agacaaattt atgagcgttt catcatttcg aagaaatcgt cattggtctt
    25321 ggtcatggcc agtttattaa tgaggaattc cattgcatcg atttcgccca tcgggtgaat
    25381 gattttgcgc aggatccaca ttttctgcag ttcttcctga gtagtgagca gttcttcttt
    25441 acgggtaccg gaacggttgt agtcgatagc cgggaagacg cgtttttcag cgatcttacg
    25501 agagaggtgc agttccatgt tgcctgtacc tttaaactct tcgtagataa cttcgtccat
    25561 tttagaaccg gtatcgataa gcgccgtcgc gataatagtc aggctgccgc cctcttccac
    25621 gttacgcgcc gcaccaaaga agcgtttcgg acgatgcagg gcgttggcat ccacaccacc
    25681 ggtcaacact ttacctgacg ccggaacaac ggtgttgtaa gcgcgcgcca gacgagtgat
    25741 ggagtcgagc agaatgataa cgtctttctt gtgctcaacc aggcgtttgg ccttctcgat
    25801 caccatttcc gcaacctgaa cgtggcgaga tgcgggttcg tcaaaggtag aagcaacaac
    25861 ttcacctttt accagacgct gcatctcggt tacttcttcc ggacgttcgt cgatcagcag
    25921 aaccatcagc acacaatccg ggtggttgta agcaatgctc tgggcaatgt tctgcagcag
    25981 catggtttta ccggctttcg gcggtgccac aatcagacca cgctgaccac gaccgatagg
    26041 tgatgccaga tccagtacgc gagcagttaa atcttcagta gaaccgttac cacgttccat
    26101 acgcagacga gaatttgcgt gcagcggggt taagttctca aagaggattt tgttgcgggc
    26161 gttttcaggt ttgtcgaagt taacttcgtt aactttcagc agcgcaaaat agcgttcacc
    26221 ttctttcggc gggcgaatct taccagagat ggtatcacca gtgcggaggt tgaaacggcg
    26281 gatttggcta ggggaaacgt agatgtcatc aggaccggcg aggtaggagc tgtctgcgga
    26341 acggaggaaa ccaaatccat cctgcaatat ctccagtacg ccatcaccaa agatatcttc
    26401 gccactcttt gcgtgctgct tcaggatggc aaaaataatg tcctgcttac gcatgcgagc
    26461 caggttttcc agccccatat tttcgccgag agtgatcagc tcagaaaccg gcgtattctt
    26521 taattcggta agattcatag tggtgtgagt tcttaaactt ggggtaaatc tcgaacttaa
    26581 tgttgtgaat ggtatggcag gatcatccat gcctgtttac ggtcatcgac tcatgtcttt
    26641 tcgctgcctg gtcacaggag agtacgcaga actgaaacga caagacggat tgagtgacga
    26701 gccagaaatc tgttcacttc gcatttaaga taaaacggga agcgttggaa attacaagat
    26761 tcaaacttaa taaggtatgt ttaatacgaa gtcaacacta agttagcatg actcacgccg
    26821 ggcgtccagt ttttagcgac ggggcacccg aacatgaaat tcccttacgc caggttagcg
    26881 tcgaggaact ctttcaactg acctttagac agtgcgccca ctttggttgc cgccacttca
    26941 ccgtttttga acagcagcag agtcgggata ccacggatgc catatttcgg cgcagtgcca
    27001 gggttttgat cgatgttcag ttttgcaacg gtcagtttgc cctgatattc gtcagcgatt
    27061 tcatccagaa tcggggcgat cattttgcac ggaccgcacc actctgccca gaaatcgacg
    27121 aggatcgccc cgtccgcttt gagtacatcc gtgtcaaaac tgtcgtcagt caggtgaata
    27181 attttatcgc tcatatataa ctccacagga ataagcctgg cgtgttggtt tcgttgttgg
    27241 tgtaacatta accaactaaa ggttgacttt atttcaccgg atacgctttc gtaaagcaat
    27301 agtaagctga tattctacca cactatgagc aaaacacatt taacagaaca gaagttttcc
    27361 gacttcgccc tgcatccgaa ggttgtagaa gcccttgaaa aaaaagggtt tcataactgt
    27421 acgcccattc aggcactggc ccttccgctg acgctggcgg gtcgtgacgt agccgggcag
    27481 gcgcaaaccg gtaccgggaa aacgatggcg tttcttacgt caacgtttca ttatcttctc
    27541 tctcatcctg cgattgccga tcgcaaggtg aatcaaccgc gtgccttaat tatggcaccg
    27601 acgcgtgaac ttgccgtgca gatccatgcc gacgcagaac cgctggcgga agctactggc
    27661 ctgaagctgg gtctggctta cggtggtgat ggctacgaca aacagctgaa agtgctggaa
    27721 agcggcgttg acattctgat tggcaccacg ggtcgtttaa ttgactacgc caagcagaac
    27781 cacattaacc tcggtgccat tcaggtagtg gttctggatg aagccgatcg catgtacgat
    27841 ctgggcttta ttaaagatat ccgctggctg ttccgccgta tgccgcctgc aaaccagcgc
    27901 ctcaacatgc tgttctccgc cacgctttcg taccgggtac gtgaactggc gttcgagcag
    27961 atgaacaatg ccgaatatat tgaagtggaa ccggaacaga aaacgggcca ccgtattaaa
    28021 gaagagcttt tctacccttc taacgaagaa aaaatgcgtt tgctgcaaac gctgatcgaa
    28081 gaagagtggc cagacagagc gattattttc gccaatacca aacaccgttg tgaagagatc
    28141 tggggccacc tggcggcaga tggtcatcgt gtcggtttgt tgacaggcga tgtcgcgcag
    28201 aaaaaacgtc tgcgtattct tgatgaattt acccgtggcg atctggatat tctggttgcc
    28261 accgacgttg ccgcgcgtgg tttgcatatt ccggcagtga cgcacgtctt taactacgat
    28321 ttacccgatg actgtgaaga ttacgttcac cgtattggtc gtacaggtcg cgcaggcgca
    28381 agcggtcact ctatcagcct ggcgtgtgaa gagtatgcat tgaatttgcc tgctattgaa
    28441 acctatattg gtcactcaat tccggtaagc aaatacaatc cggacgcatt gatgaccgat
    28501 ctgccaaaac cgctgcgcct cacgcgccca cgtacaggca atggtccgcg tcgtactggc
    28561 gctccgcgta atcgtcgtcg ttcaggttaa ataaaatgtc atactcaaaa tcattcaaac
    28621 tgtatcaaga tggcaagaga atgaatcccg atgagcatac tgtagtaagt gattcaggtg
    28681 aaagaatgca gccaacacag agacagattg aaggatgaag agtatgggtt ccacctcgtc
    28741 gctgtatgca gccattgatc tcggttcgaa tagttttcat atgctggttg tgcgcgaggt
    28801 ggctggaagc atccagacgc tgacgcgaat aaaacgcaaa gtgcgtctgg ctgctggcct
    28861 gaatagcgaa aatgccctgt ctaatgaagc aatggagcgc ggttggcaat gtctgcgcct
    28921 gtttgctgaa cgtctgcaag atatccctcc ctcgcaaatt cgcgttgtcg ctacggcgac
    28981 attacgtctt gccgtcaatg cgggtgattt tattgccaat gcgcaggaaa tcctcggttg
    29041 tccggtacag gtgatcagcg gtgaagagga agcacgtctg atttatcagg gcgttgctca
    29101 cacaactggc ggtgccgatc agcgcctggt ggtggatata ggcggtgcca gtactgaact
    29161 ggtaaccggc acgggtgcac aaaccacctc gttgttcagc ctgtcgatgg gctgcgtcac
    29221 ctggctggaa cgctattttg ccgatcgtaa tctggggcag gaaaattttg atgctgcaga
    29281 aaaagcggca cgcgaagtgt tacgtccggt tgccgatgaa ttacggtatc acggctggaa
    29341 agtgtgcgtt ggtgcttccg gcaccgtgca ggcgttacag gaaatcatga tggcacaggg
    29401 gatggatgaa cgcattaccc tggaaaagtt gcagcaattg aaacagcgag ccattcattg
    29461 cggtcggctg gaggaactgg agattgacgg gctgacgctg gaacgtgcgt tagtgttccc
    29521 gagtggtctg gcgatcctga tcgccatttt taccgaactg aatattcagt gtatgaccct
    29581 ggcgggcggt gcgctgcgtg aaggcctggt ctacggcatg ttgcatctgg ccgtcgagca
    29641 ggatattcgc agccgtacgc tgcgtaatat tcagcgccgc tttatgatcg atattgatca
    29701 ggcgcagcgc gtagccaaag ttgcggctaa cttcttcgat caggtggaaa aggaatggca
    29761 tcttgaagca ataagccgcg atttgctcat cagcgcctgt cagcttcatg aaatcggcct
    29821 gagcgttgac tttaaacaag cgccgcaaca cgctgcttat ctggtgcgta atctggatct
    29881 tcccggtttt acccccgcac agaaaaaatt gctggcgacg ctactgctca accagactaa
    29941 tccggtcgat ctctcatcgc tgcatcagca aaatgccgta ccgccgcgcg tcgcagaaca
    30001 actctgccgt ttactccgcc tggccatcat ttttgccagc cgtcgccgtg acgatctcgt
    30061 gccagagatg acattacagg ctaaccatga actgttgacc ttgacgcttc cgcaaggttg
    30121 gctaacccaa catccgctgg gtaaagagat tattgctcag gaaagccagt ggcagagcta
    30181 tgtccactgg ccgctggaag tgcattaatg agtaagtgcc ggatgcgatg ctgacgcatc
    30241 ttttccggcc ttgattattt ccctcgtttt gccgccatca tcgctttcag attcgccaga
    30301 tggctttgcc ctttctgcat ccgttcttcg gcgctgacca ctttgcgctc ctgttcccaa
    30361 atcagatcat cctgcggcag ctccagcaaa aagcggctcg gctccgggcg caccagctcg
    30421 ccgtactgac ggcgttcttt acacagcgta aaggtcaatt ccttctgggc gcgggtaatg
    30481 ccgacatagg ccagccgccg ctcctcatcg atattatctt catcgatgct gctctggtgc
    30541 ggcaaaaacc cttcttccat accgaccatg tagacataag gaaactccag cccttttgac
    30601 gcgtgcagag tcatcagttg cacctgatcc agctcttctt cactctcacc acgctccatc
    30661 atgtcgcgca aagtaaagcg cgtcaccacc tgggtgagcg tcatcggctc atccagttcg
    30721 ctgccttcca gcatctccgt catccagcta aacagttggt tgacgttctt catgcgcatt
    30781 tcggcggctt tcgggctggg cgatgtttcg tacagccagg attcataatc catgccgtgg
    30841 atcagatcgc gcaccgcggc aatcggctcc cgctccgcca gacgctggat ttctgccaac
    30901 cagtgagtaa agcgggtcaa tgcttcataa ccacgtccgc taagcgtctg actcaggccc
    30961 atatcaaagc tggcggtaaa catgctttta ttgcgcgtca tcgcccactc acccagcttt
    31021 ttcagcgtcg ccgggccaat ctctctcttc ggcgtgttaa cgatacgcag aaatgcgctg
    31081 tcatcgtccg ggttagtcag cacgcgcaga taagccagca agtccttgat ttcaggacga
    31141 gagaaaaacg acgtaccacc agaaattttg tacgggatgc ggttttgcat caggaatttt
    31201 tcaaacactc gcgactgatg gttaccgcga taaagaatag cgtaatcttt gtactgcgtt
    31261 ttattgacga agtgatgcgc aatcagttca ccagtgacgc gctcggcttc atgttcttcg
    31321 ttattcgcgc ttaaaacttt aagctctgtg ccgtaaccca gttcggagaa cagacgcttc
    31381 tcaaagacgt gcgggttatt ggcgatcagg atgttcgccg ctttcaaaat acgtccggaa
    31441 gagcggtagt tctgctcaag cttaatcacc ttcagcgccg gaaaatcctg actcagcagc
    31501 accaggtttt gtggacgtgc gccgcgccag gagtagatcg actggtcgtc atcgcccacc
    31561 acggtaaagc gcgcgcggct gcccaccagc agtttcacca gttcatactg gctggtgttg
    31621 gtgtcctgat actcatccac cagcagatag cgaattttgt tctgccagcg ctcgcggact
    31681 tcttcattgc gttgcagcag caacgtcggc aataaaatca gatcatcgaa gtcgagaaca
    31741 ttgcacgctt tcaggtgtgc atcatacagc ccataacaat gggcaaaaat acggtcccgc
    31801 tcgccaatcg cactggctgc cgcctgggac ggtgttttga gatcgttctt ccagttagag
    31861 atggtcgaaa tcaactgttg caggagaact ttgtcatctt caatcagccc ttcggtcaac
    31921 tctttgagca acgccagctg atcggtatcg tcaaacagcg aaaagttcgc cttcatcccc
    31981 agcgccgcat actcacgctt gatgatatcc agccccagcg tgtggaaggt tgagatcatc
    32041 agcccacgcg cctctttgcg acccagcgtc tgtccgacac gctctttcat ctcgcgcgcc
    32101 gccttattgg taaaggtcac cgccgcgata tgtcgcgcct gataaccgca accgcggatc
    32161 agatgggcga ttttattggt gatcacacga gttttaccgg aacccgcgcc cgccagcacc
    32221 aggcaggggc cggtaacgaa ttcgacagct tgttgttggc cggggtttag acgcataggt
    32281 gtattgctca atcttcgaac ggggggagga ttgtagcaga atcgcatttg aatgtgaccg
    32341 cgtgtaagcc gctaaccgat tggctgcgga tgtttaaatc agagagggaa gaccgaaacc
    32401 ggagttcatc gtgaatccat tagacttaga aaatatcggg tcgtaacgtt gtgttattac
    32461 tttttcagag tttactaagg agaggtgtac tcgtggcgat taatttcagt cctaaggttg
    32521 gtgaaatact ggattgcaac tttggtaact atcctgttag ccagaatgga cagttcagca
    32581 ccacgtatta cgatggccgc attcctcctg aaatgataaa aaacaggtta gt
//
LOCUS       DAJVRF010000032        32478 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJVRF010000032 DAJVRF010000000
VERSION     DAJVRF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32478
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.032"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            520..759
                     /locus_tag="OQN13_001379"
     CDS             520..759
                     /locus_tag="OQN13_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCS1614773.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(947..1924)
                     /gene="intI2"
                     /locus_tag="OQN13_001380"
                     /pseudo
     CDS             complement(947..1924)
                     /gene="intI2"
                     /locus_tag="OQN13_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2256..2729
                     /gene="dfrA"
                     /locus_tag="OQN13_001381"
     CDS             2256..2729
                     /gene="dfrA"
                     /locus_tag="OQN13_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA"
                     /protein_id="HCS1614774.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISRIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2824..3348
                     /gene="sat2"
                     /locus_tag="OQN13_001382"
     CDS             2824..3348
                     /gene="sat2"
                     /locus_tag="OQN13_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCS1614775.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3417..3914
                     /locus_tag="OQN13_001383"
     CDS             3417..3914
                     /locus_tag="OQN13_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614776.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            3975..4346
                     /locus_tag="OQN13_001384"
     CDS             3975..4346
                     /locus_tag="OQN13_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614777.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            4756..5133
                     /locus_tag="OQN13_001385"
     CDS             4756..5133
                     /locus_tag="OQN13_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614778.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            5156..5308
                     /locus_tag="OQN13_001386"
                     /pseudo
     CDS             5156..5308
                     /locus_tag="OQN13_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5364..6980)
                     /locus_tag="OQN13_001387"
     CDS             complement(5364..6980)
                     /locus_tag="OQN13_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCS1614779.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(6981..8507)
                     /locus_tag="OQN13_001388"
     CDS             complement(6981..8507)
                     /locus_tag="OQN13_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCS1614780.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(8510..10177)
                     /locus_tag="OQN13_001389"
     CDS             complement(8510..10177)
                     /locus_tag="OQN13_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1614781.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(10174..12282)
                     /locus_tag="OQN13_001390"
     CDS             complement(10174..12282)
                     /locus_tag="OQN13_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCS1614782.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(12269..13090)
                     /locus_tag="OQN13_001391"
     CDS             complement(12269..13090)
                     /locus_tag="OQN13_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCS1614783.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(13250..15079)
                     /gene="glmS"
                     /locus_tag="OQN13_001392"
     CDS             complement(13250..15079)
                     /gene="glmS"
                     /locus_tag="OQN13_001392"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCS1614784.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(15241..16611)
                     /gene="glmU"
                     /locus_tag="OQN13_001393"
     CDS             complement(15241..16611)
                     /gene="glmU"
                     /locus_tag="OQN13_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCS1614785.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(16962..17381)
                     /gene="atpC"
                     /locus_tag="OQN13_001394"
     CDS             complement(16962..17381)
                     /gene="atpC"
                     /locus_tag="OQN13_001394"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCS1614786.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(17402..18784)
                     /gene="atpD"
                     /locus_tag="OQN13_001395"
     CDS             complement(17402..18784)
                     /gene="atpD"
                     /locus_tag="OQN13_001395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCS1614787.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(18811..19674)
                     /gene="atpG"
                     /locus_tag="OQN13_001396"
     CDS             complement(18811..19674)
                     /gene="atpG"
                     /locus_tag="OQN13_001396"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCS1614788.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKSVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(19725..21266)
                     /gene="atpA"
                     /locus_tag="OQN13_001397"
     CDS             complement(19725..21266)
                     /gene="atpA"
                     /locus_tag="OQN13_001397"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCS1614789.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(21279..21812)
                     /gene="atpH"
                     /locus_tag="OQN13_001398"
     CDS             complement(21279..21812)
                     /gene="atpH"
                     /locus_tag="OQN13_001398"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCS1614790.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(21827..22297)
                     /gene="atpF"
                     /locus_tag="OQN13_001399"
     CDS             complement(21827..22297)
                     /gene="atpF"
                     /locus_tag="OQN13_001399"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCS1614791.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(22359..22598)
                     /gene="atpE"
                     /locus_tag="OQN13_001400"
     CDS             complement(22359..22598)
                     /gene="atpE"
                     /locus_tag="OQN13_001400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCS1614792.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(22645..23460)
                     /gene="atpB"
                     /locus_tag="OQN13_001401"
     CDS             complement(22645..23460)
                     /gene="atpB"
                     /locus_tag="OQN13_001401"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCS1614793.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(23469..23849)
                     /gene="atpI"
                     /locus_tag="OQN13_001402"
     CDS             complement(23469..23849)
                     /gene="atpI"
                     /locus_tag="OQN13_001402"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCS1614794.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(24466..25089)
                     /gene="rsmG"
                     /locus_tag="OQN13_001403"
     CDS             complement(24466..25089)
                     /gene="rsmG"
                     /locus_tag="OQN13_001403"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCS1614795.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(25153..27042)
                     /gene="mnmG"
                     /locus_tag="OQN13_001404"
     CDS             complement(25153..27042)
                     /gene="mnmG"
                     /locus_tag="OQN13_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCS1614796.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(27421..27864)
                     /gene="mioC"
                     /locus_tag="OQN13_001405"
     CDS             complement(27421..27864)
                     /gene="mioC"
                     /locus_tag="OQN13_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCS1614797.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(27954..28412)
                     /gene="asnC"
                     /locus_tag="OQN13_001406"
     CDS             complement(27954..28412)
                     /gene="asnC"
                     /locus_tag="OQN13_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCS1614798.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            28564..29556
                     /gene="asnA"
                     /locus_tag="OQN13_001407"
     CDS             28564..29556
                     /gene="asnA"
                     /locus_tag="OQN13_001407"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCS1614799.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(29561..31012)
                     /gene="viaA"
                     /locus_tag="OQN13_001408"
     CDS             complement(29561..31012)
                     /gene="viaA"
                     /locus_tag="OQN13_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCS1614800.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(31006..32463)
                     /gene="ravA"
                     /locus_tag="OQN13_001409"
     CDS             complement(31006..32463)
                     /gene="ravA"
                     /locus_tag="OQN13_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCS1614801.1"
                     /translation="MSSSLEKGLYERSHAIRLCLLAALSGESVFLLGPPGIAKSLIAR
                     RLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTSGYLPEAEIVFLDE
                     IWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEADSSLEALYDRMLI
                     RLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKEIGEITLPDHVFEL
                     IFMLRLQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVDLILLKDCLWYDAQ
                     SLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKTALTVIRLGGIFSR
                     RQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLSKGGEIRGKLNGIG
                     FAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQLEELESDWRKQHAL
                     FSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
BASE COUNT         8282 a         8219 c         7527 g         8450 t
ORIGIN      
        1 tcggcagccc tgtgggcgga caaaatagtt gggaactggg aggggtggaa atggagtttt
       61 taaggattat ttagggaaga gtgacaaaat agatgggaac tgggtgtagc gtcgtaagct
      121 aatacgaaaa ttaaaaatga caaaatagtt tggaactaga tttcacttat ctggttaact
      181 aatctagcta acctgccgct cagcttagta cgatcctata tccaactgat cataatcctt
      241 tcatcagtat cccaaattaa atccccctcc tagactgcct caccaacact ttacagtgat
      301 cccgtatttc cggacaggtg attgattttt ttcgcttgta caggtgttaa gccacagtta
      361 tcttgatgtg gacattatgc attgtaatag cccattaaca tgcgctaaga gcataaatta
      421 atatacagta acaagcatta cgaggtgcta atgtttggaa agcagcctct tccaactaca
      481 ataataagtt aactatcctc cacccacaaa taattacaaa tgaccccttt ccgctataac
      541 tccgatctca ccagcggctc gctacaaacc cgagagtgtc gcattattac tggcttgtta
      601 ctccaagagc ttgatgaagc tgcctgggat aaagccatgt ataaggaaaa tgtgcttcag
      661 aaacgcacgc aatctacggt aagacgtatt tcttcagcgc ttagaaaacg cctagaacac
      721 ctaagctctg acttttgggc ttttgcgttt ttatgttagg cattgtctgg caactggagg
      781 aagtgtaatg accatgaatg gtcactgtga ttgacagtat cttgcttaaa cttatctgaa
      841 caatggtaga actggctcat atttatcccc tctatgattg tttatctttt tcaccataaa
      901 aagtatcagg ggctactcag ggctacataa agatatttat ctacttttac tgattgataa
      961 gtagcatcag tccatccgca ggactggtgg tgccggcaaa atgctgaccc aacacatgcg
     1021 tatagatttg cgtggtctta acatcgttat gccctaagag ttcttgcaca gtgcgaatat
     1081 cacgccccgc ttgtaataga tgcgtagcaa acgagtgacg aaatgtatga caagtgacac
     1141 gcttgctaac gatgcctgct ttttgtacgg ctgccttcaa tgcctttcgc gcaacggagt
     1201 catgcagatg atggcggcat aatttgccgt tatacgggtg gttgcagagc gtgctggagg
     1261 gaaagacaaa catccacgcc gcttgtcgat aagcagaagg gtatttgtga tctaaagcaa
     1321 aaggcagcga tggccctacg ccttgtaagt tgtcgtcttg ctgaataagc cgcgcttgct
     1381 caatgagtta ttttattgct gggattaggc gcgtgggcag taggctgttt ctgcttttcc
     1441 cacccttacc gtcatgcaca gtgatgcagc cattatcaaa atcaaaatct ttaacccgca
     1501 aacgcaagca ttcattaatg cgcaaacctg caccatacag cagcgtaaaa ataacttggt
     1561 tgcgagtatc cataacctgc aaaatgcgtt gcacttcatt tgcagagata acagagggta
     1621 gccgtctagg cttgcttgca gggatataat caatatcgcc caacggctgt tgtaaaaacc
     1681 tgttgtacaa aaaagctagg gcatttaaag cgattttctg cgtgtttatg gctacatgtc
     1741 tgctgtttgc taagctggat aaaaacagcc tgacctcttc actgcccatg gtctgaggat
     1801 gacgtttttt gtgaaacaga ataaaacgct taatccagtg caggtaagtt ttttcagttt
     1861 tcagcgcata acctttttgt cgcatatccg tgcgtataga atttaaaaat ggactgttag
     1921 acataaaacg ctccttgtct tgcaactgtc tgcctataca gcctattcta gctgggattt
     1981 aaaaaagtgc ctgtttttta cgcctagaga tgcttgttta ccggtagagt tttaatttaa
     2041 tgctaaataa attaaaatgt tatgagttct ttgggtgaga taatgtgcat cgtgcaagca
     2101 ggatagacgg catgcacgat ttgtaataac agagtgtctt gtatttttaa agaaagtcta
     2161 tttaatacaa gtgattatat taattaacgg taagcatcag cgggtgacaa aacgagcatg
     2221 cttactaata aaatgttaac ctctgaggaa gaattgtgaa actatcacta atggtagcta
     2281 tatcgaagaa tggagttatc gggaatggcc ctgatattcc atggagtgcc aaaggtgaac
     2341 agctcctgtt taaagctatt acctataacc aatggctgtt ggttggacgc aagacttttg
     2401 aatcaatggg agcattaccc aaccgaaagt atgcggtcgt aacacgttca agttttacat
     2461 ctgacaatga gaacgtattg atctttccat caattaaaga tgctttaacc aacctaaaga
     2521 aaataacgga tcatgtcatt gtttcaggtg gtggggagat atacaaaagc ctgatcgatc
     2581 aagtagatac actacatata tctagaatag acatcgagcc ggaaggtgat gtttactttc
     2641 ctgaaatccc cagcaatttt aggccagttt ttacccaaga cttcgcctct aacataaatt
     2701 atagttacca aatctggcaa aagggttaac aagtggcagc aacggattcg caaacctgtc
     2761 acgccttttg taccaaaagc cgcgccaggt ttgcgatccg ctgtgccagg cgttaggcgt
     2821 catatgaaga tttcggtgat ccctgagcag gtggcggaaa cattggatgc tgagaaccat
     2881 ttcattgttc gtgaagtgtt cgatgtgcac ctatccgacc aaggctttga actatctacc
     2941 agaagtgtga gcccctaccg gaaggattac atctcggatg atgactctga tgaagactct
     3001 gcttgctatg gcgcattcat cgaccaagag cttgtcggga agattgaact caactcaaca
     3061 tggaacgatc tagcctctat cgaacacatt gttgtgtcgc acacgcaccg aggcaaagga
     3121 gtcgcgcaca gtctcatcga atttgcgaaa aagtgggcac taagcagaca gctccttggc
     3181 atacgattag agacacaaac gaacaatgta cctgcctgca atttgtacgc aaaatgtggc
     3241 tttactctcg gcggcattga cctgttcacg tataaaacta gacctcaagt ctcgaacgaa
     3301 acagcgatgt actggtactg gttctcggga gcacaggatg acgcctaaca attcattcaa
     3361 gccgacaccg cttcgcggcg cggcttaatt caggagttag agagctgggg aagactatgc
     3421 gcgatctgtt gaaggtggtt ctaagcctcg tacttgcgat ggcatcgggg caggcacttg
     3481 ctgacctgcc aattgtttta gtggatgaag ctcgtcttcc ctatgactac tccccatcca
     3541 actacgacat ttctccaagc aactacgaca actccataag caattacgac aatagtccat
     3601 caaattacga caactctgag agcaactacg ataatagttc atccaattac gacaatagtc
     3661 gcaacggaaa tcgtaggctt atatatagcg caaatgggtc tcgcactttc gccggctact
     3721 acgtcattgc caacaatggg acaacgaact tcttttccac atctggcaaa aggatgttct
     3781 acaccccaaa aggggggcgc ggcgtctatg gcggcaaaga tgggagcttc tgcggggcat
     3841 tggtcgtcat aaatggccaa ttttcgcttg ccctgacaga taacggcctg aagatcatgt
     3901 atctaagcaa ctagcctgct ctctaataaa atgttaggcc tcaacatcta gtcgcaagct
     3961 gaggggaacc actagtgtca tacgaacctc caagagacgg ttacacaaac gggtacattg
     4021 ttgatgtcat gtatgacaat cgcccaagta agtatccagc tgtgttcaga acgtacgtcc
     4081 gaattccaga ctatgtcgaa ctagataacg cggaggagct ggccgacgca atcattcgag
     4141 aggttgagtt aaaggaggca tggctcacaa gggtcttggg gaactcagcc gatcctgacg
     4201 cttctgtggg caagtgggac cggcgcctag cgcagtgcgg tggtattgat gagatgtcaa
     4261 gtggggtgca ggcaacatac ttcagtccaa acggtcagct ggtcggctta gtggcataca
     4321 ttgatgccga atggatggat gaatagctct gacgttacga ttcaggtgag acaaccagat
     4381 gggcattgcg tacaagctca gttgccaccg atgtggctac gagtctgatc ttctttacct
     4441 aggccaaggt atggcgatgc ttcctgagca aatagtcgga acgtgcacat gctgcgacaa
     4501 tctaacgacg atttctataa atgaaagcta cggagtatgc tcactttgtg gggctaagga
     4561 tcgtgtgata tttacaagct atcagcatga ggcaaggcca agaaatccct gctatctaga
     4621 gagaagtttc aaatatcaat gcccaagatg ccatgccttc agtatggagc ctccggatct
     4681 accggagatg ctttgggact aatgctccgc tttgcctcgg gtatccgttc gacctatgtt
     4741 taagaggaaa cgcacgtgtc tgccgccaaa gctcttgcct atgcactttg tgacaagttg
     4801 gaaagcaaaa aagttgatga agtcgtacga aatcttgttt ctagcggctg gaatattagg
     4861 gaagtggcct gggaagcttc gtcctttgat catgaaatgc cctactttga cacggggcgg
     4921 gacttcgata ttggatttcc tgacacgctg tcatccaaga ttgagttttt gaccgcgaat
     4981 ggatcaatcg tccaagcacg tgctcgcctg cacttcaagc aggctctaat ttttgccaag
     5041 gcgtccaaac atgtcgccgc gctcaagaac tcattggatt actactatgg atcgtctgta
     5101 atcccccctc aaaaccacgg aggctatttg tagaaaattc cattaatatg aactcaaggt
     5161 gttccctacg gacgaatcag cgatgaaggt ggtgtacctg gctatccagg cggcgaccaa
     5221 gaaatggacg atgccgatcc gcaactggaa gccggccatg aaccgtttta tgatcgaatt
     5281 tggtgaccgt ttaaatggcc acctttaact gaccggcaaa agcacttaca caaaagggtt
     5341 tacaggctct ttcagtcatt cctttaatgc gtaaattgct ctctaaccac tctctcagcc
     5401 cacccttcca tatcctctct cgatacttgc agctccccca acccctttgg atgattcacc
     5461 cctctatgcc cgtcttgtcc atataattga tcaaacaaac tatttggcca attcaatgag
     5521 ctcttcacca ctccacgccg tatcttttca aaatcattcc gccatgtttc gctatcaacg
     5581 ccactcaaca ctttggtaga aagcattttc accccatccg atgcatccac ctctagcaat
     5641 acaaattcac tgccattacg ttgcacccca acggctttaa tcactcttgg tgagccatct
     5701 ttacacagat gtaatcggct acgcccaact tttggcaaaa ccaaggtttc ttcaaaaagc
     5761 acccgacatg caaatttagt ttttagaatt gaaagtagct catcaaaagc agcaaatcga
     5821 ttagcaaaaa tagagttgta attggttgca tcctgcttcc cacccacatc tgccgctgct
     5881 aggacaccac ctaagtgtgg ctcatcagta ctaaccaaat tactattttc acttgttgtt
     5941 gcttcttctt tttctaaaat agtttggcta ctttttcttg ccctgcgaga tttttcaatt
     6001 cgactaggtc tactaaaact tatccatgtc ggctcagcct ctatgactcg tgtgtctgtg
     6061 tctatattgg ctgtttcctc atcgtccaac tgtagatgct catcatcaac aactgactct
     6121 gttgaaaccg ctatgtgctg cgtactacca tcacctgcct ccttttcctg aaaagaggca
     6181 tggctaatag ctgttgtgct aggaagcata gcgttgattt ctaaacctac tatttcctca
     6241 actaaataat ccttatcctc gttagaagaa cgtcctttta catgtaattt ccaaccctgc
     6301 atgggcggag ggtcaaagct aaagcaccag ctttcaacgc cgttcttaat ttctctattt
     6361 tgttgatagt gcctaaaaat actttcatac gaatccataa catcttgatc cgaaaacaac
     6421 caaaccaaaa gctgcactac ggccgactgc tctaacgccc ctttaggaaa cgatgaactg
     6481 ggtaagatcc ttatctctaa atgatctcgc tcaacctcat actgaacgtc gaactcttgc
     6541 tgtaaagcgg tactgctcaa acagcttcga cagaaatagg agttaattaa aaataatgac
     6601 cgagctagct ctaactgtgg gatgtgataa cgagtcttgc tatatggtgt ttcagcttcg
     6661 taaataaatg aactctgcgc tccatccttg ttgcgtacgg aagcaaagga cgagagatta
     6721 ggaaaatcga ttattttggc tcgttgaaga tccgatgctt caaactcaat cacacgatcc
     6781 gccttattta tttgctttgt tgtactgtta accaccttag ctcgacttaa taaaggcaaa
     6841 tgagtaaatc gagtccattt ccgttcttgc attggattaa accaaacaaa aatacgccac
     6901 tccttatgac ccgagttacg gaataaatga ccaatatgta caacctgcac attgtcatta
     6961 aatgtagcta gcctaaccac tcactcttcc ccataaacca tttccaagaa tctttgtgct
     7021 tcctcagtta tccgctcttt agataacgtt gcacttctta ataatcgcca gcgcctaagc
     7081 tcaacatctt cctgtttaag cttaataaaa gcttggctaa tccgtctaat ttgataatct
     7141 tccacactct ctgagtaacg ctttaagcaa agcgctacca aaggcagttt ctgtagattt
     7201 tttgcaagag aggttccgtt aggagtttgc tttaacagcc agctcgatgt cgctcttggg
     7261 tgatcaaggc tactatctag acgctttatg attcttaata gttgtcgtac agcaattcga
     7321 tctctttggt tccaatcaac tctaggggcg ggtgccagac gctcttgttg atgctgttga
     7381 ttccagtgaa ctagccaatc cctgtcatgt cggtaaagcc aagcgtatag caccccaccc
     7441 tctaaagact gtcttgccgc cttaattcct tggtatttat gcactagttg ctgccagtct
     7501 ttatgcttaa cagataaatc ttcagagtta ggttgcacag actggctaac aggtcttgtc
     7561 gttatagagt gctcagtaag agcacttgcc tgctgtagcg cttctataac cgttagtttt
     7621 ggcaataagg cttgccacac aatactatgc tgtaaataac taaaggcttt tctatgctta
     7681 cggaatatac ttttcagcca acacgtgtcc ttgttctctg ccaactttaa atccagtttc
     7741 tctagtgcct catcactaaa agtctgcctc actctctccg ccaccaagtc atgacgaatg
     7801 tgcttgcttt tggttagccc tagatcctgc gctaagcgct gataaaacag cgtccactgc
     7861 tcaaggcttg gggaaagctc ttgcgctcgt ggagcatcta acagaggggc tatataagca
     7921 gctagtgctg ttaattgaga tagggagtct ttggggtagt ctgaaagcag ctcagtatga
     7981 cccaaagccc aaaattgatg tcggtgatca tctacagctc tatcaaagaa gactaaagca
     8041 ccgtgttttg gacaatatgg caaagcgggc aaataccaat ctcgttgcca aaaggcttcc
     8101 ccatacctat ttagctgaag agcaacgcaa tcagggcagt atctaaagcg gttatcgctc
     8161 ttaactctag aagcagcgac tcctagcatt aaatgcaccg caccttgcgc ttggtactcc
     8221 attaaccgaa tagcttcgtc tcggcgctcc ttgcctacaa acggagcata taaagggaat
     8281 aaggtatgct cataaataag ctgctgaaca gcgtaacgtc ctgtttgatg tagatgtctt
     8341 gctatcacac ctaaatgcga gggcagacct aaggtagcga ccaccttgcg gttgccatac
     8401 acctcatcca acagctgctt aggactaaca atcccttgat aaacgcctgc ccgtgcaata
     8461 gtgctataaa tcagctcatt cgagtacgga acaggaaagt ttctcatggc taacctgctt
     8521 gtttaaataa gctcgccata tccactatta cccctttccc ttttaaccgt tcatgcatgg
     8581 ttttttcagg ttggcgttgt gaatagatat aacgtaaatc cgtatcaggc aagctatccc
     8641 agtcgctggg cttgactacc tttatagccg aaaccttttt actcttggag ggcttttcta
     8701 gttctgacac taccgtttcg ccttccatca accactgcaa aacaagaggc agtaactttt
     8761 gtcttgtcat cgttggattc tggctaaacg cttttttaat agtgggaatt aacaggcttg
     8821 aatcgtaatc ctctttcagc atcagatata aatgacgctg atcttcggta tctaactctt
     8881 gaagggcttt ttcttctggt gtttgttctt gtatcgctgc gatatctagc tgaagttgga
     8941 ttaaccgttt atcaatctcg ggaacgacta gatcagaata acgagcaatg cgttctggga
     9001 tacccgagcg taatgcctct agcatggggt gcacaggctt taactcatct tgatacactt
     9061 gccgcaataa accagcggta atacgctcat tgcctaaagc tagcgcacgg agctgagcga
     9121 gtacaaaaag ttttactaca atgtccatca ctccttggct tagctcatac cacacatcac
     9181 ggacctcatc cgataacagc gcatctttgc gttgtaaaag ctgtaattgc caaagattat
     9241 ccgtaaaagc gatccactct tgattgggct ttccacgttg cgtttgttgt ataggatccc
     9301 agaatatagc tccaaaccct gcccctctac gtgcagaccg caaatcagcc tcaaaaatct
     9361 ctcgtgcttt aggggtacca atcaacatca ctggtacgcc aataatattc accatcgtca
     9421 caaaaaagtt cagcatctct tgagatccac ccgaacgaga gcggcttaaa tgctgaattt
     9481 catcaataac caacaaccct aaagcatgtg cattggctat ttgcgacatc aaagccaaca
     9541 tggtttctat accatgacgt tttaagccat aacgacgctc atagttcgag cccaaggctc
     9601 gatccaacgc tctgaaaaaa ttcaagcaga tttcttttag cgaaccatta tgcgagcagt
     9661 ctattttcaa atacaccacc tgctctacat tgagttcacg atggtaaatc acctgaggat
     9721 acgtggctag aatacgatga agagaggtcg tcttcccact accagaacaa ccaattaaca
     9781 ataagctttg tgccgtagat cgtgcctcct caaagcgaaa tgtctccaac tctcccgttt
     9841 gaacacgctc ataaccattt tgtaaatgct tttgtaaatc tcctgtttta ggatttctgc
     9901 ctacgtagcc acctcgaatc atgaccgaaa tacgctcact tagtagcaaa tgcgtaccta
     9961 atggctgaaa atagtcatct ggaatacgac aaatggtatg agctctgata acacgggact
    10021 tttgcaagtc agaggaagta agctgtaaag aggatttcag tgatgcagca ctattcactg
    10081 actcttgtaa tggtggtaag gcctcgataa aaggattatc acgataagcc tctacccccg
    10141 tatcacgata aactgcttga atccgggtag cactcatgac tcatcctttt ctggtggatc
    10201 ctgaaataat tcaggcacgt atgtgggtag gctgtaatct tcttgatcat ccgcttccac
    10261 tgcgttgaaa ggaataactt tagcctcatc acctgaagag cttggcttca aatgctccgc
    10321 acgtttttta cgctccgagg tcacggcttc ttttttatta gttttaatct gcttaatgcg
    10381 tgttgatttg ggctcagtag tactgggcgt taacttattc gctttctgaa tggtttgctg
    10441 aataaacgcc tcaagctccc tgcgtttagt taactcatcc tgcttcgcat tggctttatt
    10501 gtgtttttct tgtgcttgta tatcccaaac ctcccaaaat gagagacctt taaactgccg
    10561 actacgttcc gtcagattac agcgccaaaa tacacggctg ccaacttgcg gaaacaaata
    10621 aatcgtatca accagcactg ggtcataagc cgcttctaaa tgttgaggtc tagctatatc
    10681 agtgctccgc tgcaaccaac cctcacgcag aatctctgac cccgagtaat acaaacccca
    10741 caaattaacg ccaaatgaag aaatagagac ctttcggcga ggcagtaacg ctactcgcaa
    10801 ctgctcttgc tccacagccc ttaaactacc tgtacgatgc tgcatacccc attgccatag
    10861 ctggacagga atagacggta aatctgtagg aaaatcagca tctcgatcgt atttatccat
    10921 caccagatgg ttatttctga ataagatcgt acgcaaaata atttgtgtga actcaaatac
    10981 cgacagagat gcatctaacc tatagtctgt ttcaccatgg cttttgatcc gactgccctc
    11041 tacaatgcca ggtgcaaagg acttaaactc ggcttgtagt gttctaaaag tgctttccac
    11101 tatgccttta gcatcgccac gtctaggtgg agcactttcc actcgcacat taaaactaga
    11161 aactaaggct tcgacctgat gactcattaa ttcgccacgg tccgctagca acacatctgg
    11221 caaacctaca cacggccagt ctgagctact aatctcaata tcatgctggg cacaaatggc
    11281 cgttttgtca gagcaagcat ttacaaaagc ctgcatcgcc accacataag acggattttc
    11341 aaagccgata taaaagcccg tgatcatccg actaaacaca tcaatcacaa tgtaaagcgt
    11401 tggtcttcct atgatttttt ggcgatcatg atgatccact aaataaatat cagcaatcgt
    11461 ggcatcaatc tcataacgac tcccagggcc taacgcctga gaagtggctg tactacttaa
    11521 gggtcgtacg tcttttttat atacccctgc tttaactcta gactttaagc gctgagcttt
    11581 agggtattct cgatcataaa aataacgaaa ctgacgtagt gttgggtaat cctcttgggg
    11641 aatgcgagga aaatactgag caaacaagtc cacaaatcgt ctataggcaa cggtggtctt
    11701 tgtacctttt tgatttaaca ggtgcttttc tatggtcaac ctaaaaaggc gttcaatctc
    11761 gggcgttacc ttggttcctt cacccttacc atattctctg gctcggccaa tctttgctgt
    11821 tcctgtcgct gaacgtcttt cccctggtgc accgctgttt ttgtagtcag gaattaatgc
    11881 attaggcgtt tgaccacgct gccagtaacg gcgtaacaac ttataaactg tagccttagt
    11941 aaccttatgt tcttggacca catgctcaac gagttcgctt ctgactttag ggtcgaaacg
    12001 atccttacta ttaataatag gaagaatttt tcgataatcc tcctcgcgct tctggaagct
    12061 gacagaatct acagacggct cttctaaatc aagatgtaca taagggtcat cagttctaac
    12121 taagcggcct tcatctaaat actgcattag caacaactca gccctagctt gtggaactcc
    12181 tttatcagcg cttatttgca tccagacaac ttggccatcc tctatagcca aaatgcgata
    12241 cggatcatta tcaaatagca caacctcatt aatttgccac atagcgcaac tcctccatat
    12301 tcactacttg gctaatacag agatctgcac acttatttgc cctgaaagac ttataaatat
    12361 tgaacttaat aaaaccattt gcggttaagg ctcgtatctc actcaatgtt ttgcctaact
    12421 ccaaatcata agcaatatca acctgcttac agacattgat aatgttttca tctccttttt
    12481 cttgcaggat atgggccaat ggggatagtt gtgctaaaag ctccgcagaa acttcttctg
    12541 ttttcactga ataaagccat tcaatatttt cttttactac gggatttatt tctttatcag
    12601 taaaaatgaa ccaaggaatt tgcttttgct gccaatagcg acgctctagt tctagttttt
    12661 ctaaggtacg ctcgtcttgt aaggctgctg caggtttgac ttgaatagca aactgctcaa
    12721 aaggaccatc tttgcagtcc actaaaaaat cagtagacat aacctgatct acaccacgaa
    12781 taacaggatg cttaatacca ctatctattg caatctgcct ggtatcacta ggtaataagg
    12841 ggaactgctc gcgtatatct agcacgctgc tctcccactc aagactgaga aaaacagcaa
    12901 gctctaagtc agatagcaaa tgatggactc gtcccgtctt atgagaataa atacggtggg
    12961 aacgacctga agaaggaact tcttgtactg ttagccatgg aatatagtct ttaccatgcc
    13021 cttggccacg cccctcttta atacgacggg caatttgcac ttcagaaaat gaagagtttg
    13081 ctttagccat aacaaaagtc cagtatgctt tttcacagca taactggact gatttcagtt
    13141 tacaactatt ctgtctagtt taagacttta ttgtcatagt ttagatctat tttgttcagt
    13201 ttaagacttt attgtccgcc cacaagccct tttttatcta tgatagtgat tactcaaccg
    13261 taaccgattt tgccaggtta cgcggctggt caacgtcggt gcctttgatc agcgcgacat
    13321 ggtaagccag cagctgcagc ggaacggtgt agaagatcgg tgcaatcacc tcttccacat
    13381 gcggcatctc gatgatgtgc atgttatcgc tacttacaaa acccgcatcc tgatcggcga
    13441 agacatacaa ttggccgcca cgcgcgcgaa cttcttcaat gttggatttc agtttttcca
    13501 gcaattcgtt gttcggtgca acgacgataa ccggcatatc ggcatcaata agcgccagcg
    13561 gaccgtgttt cagttcacca gcagcgtagg cttcagcgtg aatgtaggag atctctttca
    13621 gcttcaatgc gccttccagt gcgattgggt actgatcgcc acggcccagg aacagtgcgt
    13681 gatgtttgtc agagaaatct tctgccagag cttcaatgcg tttgtcctga gatagcatct
    13741 gctcaatacg gctcggcagc gcctgcagac catgcacaat gtcatgttca atggaggcat
    13801 ccagaccttt caggcgagac agcttcgcca ccagcatcaa cagcacagtt aactgagtgg
    13861 tgaatgcttt agtggacgcc acgccgattt ccgtacccgc gttggtcatc agcgccagat
    13921 cggattcgcg caccagagaa gaacccggaa cgttacagat tgccagcgaa ccaaggtaac
    13981 ccagctcttt cgacagacgc aggcccgcca gggtatccgc ggtttcgcca gactgtgaca
    14041 aggtgatcat caggctgtta cgacgcacgg cagatttacg atagcggaat tcagaggcga
    14101 tttcgacgtc gcacggaata cctgccagcg attcaaacca gtagcgggaa accataccgg
    14161 agttataaga agtaccacag gcgaggatct gaatatgctc aaccttcgac agcagttcgt
    14221 cggcgttcgg tcccagctcg cttaaatcaa cctgaccgtg gctgatgcgc ccggtaaggg
    14281 tgttttttat cgcgttcggc tgttcgtaga tctctttctg catgtagtga cggtaaatac
    14341 ctttatcgcc cgcgtcatat tgcagattgg attcgatatc ctgacgtttt acttccgcgc
    14401 cagttttatc gaagatgttt accgaacggc gagtgatttc cgcaatatcg ccctcttcaa
    14461 ggaagataaa gcgacgggtc accggcaaca gcgccagctg gtcagaagcg ataaagtttt
    14521 cgcccatacc caggccaatc accagcggac taccagaacg tgccgccagc agggtatccg
    14581 ggtgacggga gtccatgatc actgtaccgt acgcaccgcg cagctgcggg atagcacgca
    14641 gaacagcctc acgcagagtc ccgccttgtt tcagctccca gttcaccaga tgggcaatca
    14701 cttcggtgtc ggtttcagaa acgaaggtat agccacgcgc ttttaactct tcacgcagcg
    14761 gttcatggtt ttcgatgatg ccgttatgca ccaccacaat gtgttcagaa acatgcggat
    14821 gcgcgttagc ttctgaaggt tcaccgtggg tcgcccagcg agtgtgagca ataccggtgc
    14881 cgccatccag aggatgttct tccgctgcct gagccagcat ctggacttta ccgaggcgac
    14941 gcaggcgggt catatgacct tccgcatcaa caacggccag accggcagag tcatatccgc
    15001 ggtattccag acgacgtaaa ccttcaagaa ggatttctgc tacatcacgt tgcgcgatcg
    15061 cgccaacaat tccacacata gtttttgatt ccgatttata tcgttgtcgg tcaacctgta
    15121 tgcccgtttt ttcgggcgcc ccgagccttg tagagagtgg ggttattttt atagttactg
    15181 cttgtgggag ggagattatg ttatctcctc atcccagatg accggattac ccggccagaa
    15241 ttactttttc tttaccggac gacgccagcc ttctttctga gtctgcggca cacggctgat
    15301 agctaatgca ttttcgccga cattacgcgt cacagttgta cccgcagcaa tggtcgcgcc
    15361 tttgcctact gttaccgggg ccaccagctg agtgtcggaa ccgacaaaca catcgtcgcc
    15421 gataatggtc ttaaatttat tcgcaccatc gtagttgcag gtaatggttc ccgcgccgat
    15481 gttaacgtta tcgccaattt ccgcatcgcc caggtaagtc agatgcccga ctttcgagcc
    15541 tttacccaga cgcgcttttt tcatctcaac gaagttacca acgtgtgcac cttccagcaa
    15601 ctcagcacca ggacgcaaac gagcaaacgg gccaatagta caggccgctg ccagattcgc
    15661 gtcttccaca acggtatacg gactgatttc gcaatcatcg ccaatcacgc tgtttttaat
    15721 cacgcaaccg gtgccgattt tcacgcgatg accgagagtc acgttgccct cgatgataac
    15781 gttagtatca atttcaacat cgcgcccgtg agtaagcgta ccgcgcagat caaaacgcgc
    15841 cggatcgcgc agcataacgc ctgctaacag cagtttttca gcctgttcgg actggtaaac
    15901 gcgctccaga cgggagagtt gcaggcggtt attcacacct tctacttcgc ttaaacgttg
    15961 cggatgaacg gcgacgattt cacgcccttc ctgatacgcc agcgcaataa tgtcggtgat
    16021 gtagtattcg ccctgagcat tattgttggt cagcttcgcc agccagcgtt tcatatctgc
    16081 gccgttagcg atcagaatgc cggtgttgat ctcctgaatc tgacgctgct cgtcggtggc
    16141 atctttgtgc tcaacaatgc cggtaacttt gccattttca cgggtgatac gtccataacc
    16201 ggtcggatca tccagtttca ccgtcagcag accaatgcca ccctgcggtt tagcatcacg
    16261 caggcgctgg agtgtttcga cagagatcag cggcacgtcg ccgtagagca ttaaaatgtc
    16321 ttcatcatcg gcaaagaaag gtgcggcctg ctgcattgca tgacccgtac ccagctgctc
    16381 tgcctgaagc acccagttaa ggttgtcgtc tttcagcgcc tgtttaagca gatcgccgcc
    16441 gtgaccgtac accaagtgaa cgtgcgctgc gcctaattca ttcgcagcat caatgacatg
    16501 ctgaaccatc gctttcccgg caagggtatg cagcactttc ggaagatcgg aatacatgcg
    16561 cgtgcctttg cctgcggcaa ggatcactac gctcatagca ttattcaaca tacgcgtcct
    16621 gactgtaatt tgagaacgaa tttaaaccgc ttcaccttga aaaaactaca tttttttcat
    16681 cgtgaaatgg acagaggata aattgttcaa taacggatta tccccgctga cgaaagtgcc
    16741 attttcgacc atcgtcacgc cttttgtctg cagaaaatta aggcagtacc ttcatttaag
    16801 cgtcaggtgg atgtttttgc tcttatttcg atcaatgaat aaacgggaaa acaggggtgt
    16861 tttatatcag caggatctat gtgaacgctc ttcaggacgg atcacgcgta aaaaaaagcc
    16921 agcctgtttc cagactggct tttgtgcttt tcaagccggt gttacatcgc ttttttggtc
    16981 aactcgataa cgcgcagctg cgcgatcgct ttggccagtt ccgcagacgc ctgagcgtaa
    17041 tctacgtcgc cgtgagagct gctaatgtgc tcttcagcct tacgtttcgc ttccatggct
    17101 cgcgcttcgt cgagatcctg gccgcgaatt gcggtgtcgg ccagaacggt cacgttgcca
    17161 ggctgcactt caagaatgcc gccagacaga tagataaact cttcgtgacc gtgctgtttc
    17221 acgatgcgaa tcataccagg cttaatggcg gtgagcagcg gtgcgtggcc agggtagatc
    17281 cccagttcac cttcgctacc cgttacctgg attttctcga ccagaccaga gaacatttgt
    17341 tgctctgcgc tgacgacgtc caggtggtaa gtcattgcca tatcaccctc cgattaaggc
    17401 gttaaagttt tttggctttt tccacagctt cttcgatgga accgaccatg tagaacgcct
    17461 gctccggcag gtgatcgtat tcgccttcca tgatgccttt aaagccacgg atggtgtctt
    17521 tcagggagac gtatttaccc ggagaaccgg tgaatacttc tgccacgaag aacggctggg
    17581 acaggaagcg ctggatctta cgagcacgcg ctaccaccag tttgtcttct tcagacagtt
    17641 catccatacc caagatggcg atgatgtctt tcagttcctg ataacgttgc aggatggact
    17701 gaacgccacg cgcggtgtcg tagtgttcct gaccaaccac cagcgggtcc agctgacggc
    17761 tggtggagtc cagcgggtca acggccgggt agatacccag agacgcgatc tgacggctca
    17821 gtaccacggt tgcgtcaagg tgcgcaaagg tggttgccgg agacgggtca gtcaagtcat
    17881 ccgcaggtac gtataccgcc tgtacggagg tgatagaacc agttttggtg gaggtgatac
    17941 gttcctgcag aacgcccatc tcttccgcca gggtcggctg ataacctacc gctgaaggca
    18001 tacggcccag cagtgcggat acttccgtac cggccagggt gtaacgatag atgttgtcaa
    18061 cgaacagcag aacgtcacga ccttcgtcac ggaatttctc agccatggtc agaccggtca
    18121 gagcaacgcg cagacggttt cccggcggct cgttcatctg gccatacacc agggatactt
    18181 tgtcgataac gttggagtcg gtcatttcgt ggtagaagtc gttaccctca cgagtacgtt
    18241 cacctacgcc cgcaaacaca gagtaaccgg agtgctcgat cgcgatgtta cgaatgagct
    18301 ccatcatgtt tacggtttta cctacacccg caccaccgaa cagaccaact ttaccgccct
    18361 tagcgaacgg acacatcagg tcgataactt tgataccggt ttccagcagt tcctgagagt
    18421 ttgacagctc ttcgtaggaa ggtgctgcgc ggtgaatcgc ccaacgctct tcttcaccga
    18481 tctcgccttt catgtcgacc ggttcaccca gtacgttcat gatacggccc agagtcgctt
    18541 tacctaccgg gacttcgatc gggtgttcga ggtcttttac atccagaccg cgacgcagac
    18601 cgtcggagga acccattgcg atggtacgca cgataccgcc gccgagctgc tgctgaactt
    18661 ccagcaccag acgctcatta ccattttgca cctcaagagc atcgtacacg cgcggtacgg
    18721 catcctgagg gaattcgacg tcaactacgg cgccgattac ctggacaatc tttccagtag
    18781 ccatcttaaa tcctctacga aataacctgt ttaaaccgcg gcggcccccg agacgatctc
    18841 ggtgagttcc tgagtaatgc tggcctgacg agctttgttg tataccaact gcagctcttt
    18901 aatcaggctg ccgccattgt cggtcgcggc tttcatcgcc accatacggg cggcctgctc
    18961 gctggccagg ttttcaacca cgccctgata aacctgagat tcgacataac gacgcagcag
    19021 ggtatccagc aacgccttcg gatcgggttc gtacaggtaa tcccaggatt tatgtttcag
    19081 atcatcatca tctgatgccg gtaacggcag cagctggctg atggtcggaa cctgagacat
    19141 ggtgttaata aatttgttgc tgacaatgta aagcttgtcc agacggcctt cgtcgtaagc
    19201 ctgcaacatc acttttaccg gaccgatcag ttcggacagg gaagggttat cccccatgcc
    19261 ggtgacctgg gcaacaacat tgccgcccac ggagttgaag aacgacacgc ctttcgagcc
    19321 gatcattgcg aggtcgcatt gaacgctttt gtcggtccag gtcttcattt ccgccagtag
    19381 ttttttgaac aggttaatgt tcaaaccacc gcacaaacca cggtcggtcg acaccaccag
    19441 gtagcccacg cgtttaacgt cgcggtcttc caggtaaggg tgcttatatt ccagattacc
    19501 gtgtgcaagg tgaccaatca ctttgcgcat ggtttctgca taaggacggc tggccgccat
    19561 gcgatcctgc gatttacgca ttttggaagc ggcgaccatc tccatcgctt tagtgatctt
    19621 ttgcgtgttc tggacgcttg cgatcttact acgtatctct tttgcgccgg ccatgagctt
    19681 ctcctcaatg ccttgcggcc tgccctaagg caagccgcca gacgttacca ggattgggtt
    19741 gctttgaagg aatcgaggat gcctttcagc ttgccttcga tttcgtcgtt gtagccaccg
    19801 gtctggttga tctcttgcat caacggagcg tgatcacggt cgacgtaagc cagcagagcg
    19861 gcttcgaagc tgccaatttt cgacagttca acatccgcca ggtaaccacg ttctgctgcg
    19921 aacagaacca gagactgctg cgcaacggac atcggcgcat actgtttctg tttcagcagt
    19981 tcggtcactt tctgaccgtg gtcaagctgc ttacgtgttg catcgtcaag gtcggatgca
    20041 aactgagaga acgctgccag ttcacgatac tgtgccagag cggtacggat accaccggac
    20101 agttttttca tgatcttggt ctgtgctgca ccaccaacac gggatacgga aatacccggg
    20161 ttaaccgcag gacgaatacc ggcgttgaac aggttggttt ccaggaagat ctgaccatcg
    20221 gtaatggaga ttacgttggt cggaacgaac gcagaaacgt cacccgcctg agtttcgata
    20281 atcggcagtg cggtcagaga accggttttc cctttcactt cacctttggt gaaggcttca
    20341 acgtattcgg cgttaacacg tgcagcacgc tccagcagac gagagtggag gtagaaaacg
    20401 tcgcccggga atgcttcacg tcctggcgga cgacggagca gcagggagat ctgacggtaa
    20461 gcaacagcct gtttagacag gtcatcgtaa atgatcagcg catcttcacc gcggtcacgg
    20521 aagtattcgc ccattgcgca accggcatac ggtgccaggt attgcagtgc agcggattca
    20581 gacgcggttg ctaccacaac gatggtgtta gccagtgcgc cgtgctcttc cagtttacgt
    20641 accacgttag aaatggtgga cgctttctgg ccgatagcga catagataca tttgataccg
    20701 gaatcgcgct ggttgatgat ggcatcgata gccagtgcgg ttttacctgt ctgacggtca
    20761 ccgatgatca attcacgctg accacgaccg attgggatca tggagtcaac ggctttataa
    20821 ccggtctgta ccggctgatc tacggactga cgttcgataa cgcccggagc gattgcttct
    20881 acagcagaga agccgtcgtg atccagcgga cctttaccgt cgattggtgc acccagagtg
    20941 ttaaccacac ggcccagcag gccacggcca accggaactt ccaggatacg gccagtacac
    21001 ttaactttca tgccttcggc aaggtcagcg tacggaccca taacaaccgc acctacagag
    21061 tcgcgctcaa ggttcagtgc gatagcgtaa cggttacccg gcagggagat catttcaccc
    21121 tgcatacaat cggccaggcc gtgaatgcgg ataacaccgt cacttacaga aacaatagta
    21181 ccttcgttgt gagcttcact cacaacattg aactgagcaa tgcgctgctt gatcagttcg
    21241 ctgatttcgg tggaattcag ttgcatgctc cagtcccctt aagactgcaa gacgtctgca
    21301 aggcgctcaa gacgaccgcg tacgctgcca tcaatgacca tatcacccgc tcggatgata
    21361 acgcctgcca ttacagactt atcgattttg caattcagct taactttgcg tgacagacgt
    21421 ttttccatcg cagcagaaat tttcgcgagc tgttgttcac tcagtgcggc agcggaaatg
    21481 acgtctacct cagcggtagc ctcactcacg gcacgaaggt gaataaactg ctccagaaca
    21541 tccgggagcg cgttaagacg accattttca gccattaccc gaatcaggtt ctgaccgttt
    21601 tcgtccagtt gctcaccaca aactgcgata aacgactcgg cgagcgtttc tggcgcaagc
    21661 gcgccagaga gaagctctgc catttgttcg tttttggtta cctcggcggc aaacgccagc
    21721 atgtcctgcc agcgttctac actttggtgt tcgacggcaa agtcaaaagc tgctttggcg
    21781 taggggcgag ctaccgtaat aaattcagac atcagcccct ccctccttac agttcagcga
    21841 caagtttatc cacgatgtcg ctgttagcag cttcatccac ggaacgttcg atgatcttct
    21901 cggcgccagc aacagccagg atagcaactt gcttacgcag ctcttcacgg gcacgtttac
    21961 gctcggcttc aatttctgcc tgcgcctggg ccacgatttt agtacgttcc tgttctgcct
    22021 cagctttcgc ttcgtccaga atctgcgagc ggcgtttgtt cgcctgctcg atgattacct
    22081 gggcttccgc tttcgctttt ttcagctggt cggtcgcgct ggcctttgca aggtcaaggt
    22141 ccttatgtgc tcgttctgcg gaagcaaggc cgtcagcaat ttctttttga cgtttttcga
    22201 tggctgccat taatggcggc catacgtact tcatgcagaa cagaacgaac aggacaaacg
    22261 cgatggcctg gccgaggatt gttgcgttaa gattcacagc acaatgcctc tatttagtta
    22321 acgttctgat attgctcttt aaataaaagc aacgcttact acgcgacagc gaacatcacg
    22381 tacagaccca gacctacagc gatcatcggg atagcatcca ccagacccat aacgataaag
    22441 aactgagtac gcagcagagg aatcagatca ggttgacgcg ctgcgccttc caggaattta
    22501 cccccgagga tgccgatacc gatcgcagca ccgattgccg ccagacccat catcacagcg
    22561 gcagccatgt acagcagatc catattcagg ttttccatga cagtctccag tttgtttcag
    22621 ttaaaacgta gtagtgttgg taaattaatg ttcttcagac gccatcgaca gatagacgat
    22681 cgtcagaacc atgaagatga aggcttgcag cgtaatgatc aggatgtgga aaatggccca
    22741 cggcacattc aggatccact gtgaccacca cggcaacaga ccagcaatca gaatgaaaat
    22801 cagctcaccg gcatacatgt taccgaacag tcgcaaaccg agtgaaactg gtttggacag
    22861 caggcttacc ccttcaagga ttaagttgac aggaatgaac gcccagtgat tgaacggctg
    22921 cagcgtcaac tctttcgtga agccgccgat gcctttcatt ttgatgctgt agaacagaat
    22981 caggataaat acgcccagtg ccatagacag cgttacgttc acgtccgcag acggaaccac
    23041 acgcagtgca ggcagaccca gtacatgttc agcaatgtac ggcagcaggt cgataggcag
    23101 taaatccatc aggttcatca ggaataccca gacgaagatc gtcagggcca gcggagcaat
    23161 cagcttgctt ttgccatggt acatgtcttt cacgctacca ttaacaaagc cgatcaccag
    23221 ctcaatcgcg gtctgaaact tacctggcac accgctggtc gcctttttgg ctacgctacg
    23281 gaataaaacc aggaacaaca gacccagcac caccgagaag aacatggagt caatattgat
    23341 tgtccagaag gtggctgggg ggttttgtgg atccaccagc gagaatgtac gcaggtccag
    23401 ctgaaggtta ttcaggtggt gtcctatgta atcctgcggc gtcatatttt ctgaagccat
    23461 gatgcctttt accctttgtt gttaattaca gccggtgcca gtatctgaac caccagcacc
    23521 aaaacccacg taacgatcag cggcaagaat accgccttta aaaccgccaa cgccaccacc
    23581 agtaacacca acatcgccag aactttgaaa gcttcgccaa atgcgaatgt ccaggccacc
    23641 cggcctttcg ctggtgtata cgcctggtga cgccaggcaa atatcataaa caaaacgtta
    23701 ggcagaaaga ctgccaggcc cccgcttatt gcagagacgc cccagaaggg gtctttgagg
    23761 ctgaacagca atccacttgc tatcaccacc agtaactgaa cgagcagaag cttccgagca
    23821 acgtttcgac tcacgagcga cacagacatc acgtttttca ctcctgctcc cttcgaggta
    23881 tgccgcgtgt cgtataaaac tttctttaag gcttagagtc aagcatcaaa aagcggtcaa
    23941 attatacggt gcgcccccgt gatttcaaac aataagtagc caaaaggtga ataaatgttt
    24001 aaatattttt ccagtgcata caattgcgac ttttccgcta accctgttcg atcatgaaaa
    24061 actgtaaata acgcgtaaac actagtgata aagcgtgctt cagatcacat attgcgcatg
    24121 ttcgcgcaca gtatatttat ttacttggca aatgatgcct ttgcaagttt atgatatttc
    24181 agtctaaaaa cagatactgt tttaataaat gacatttaca caacaaaaac cacccattga
    24241 catttttaat aatgttttaa cagccaatga tggttcttag cgccgatttt tagcagactg
    24301 atattttcac taatgactta ttttctgctt accaaaaaaa gccacgttat cttgttgata
    24361 caaaagagtg aacgtggcgt taaatgtaac cagttatatc agtagaaaac ttggttgttg
    24421 ttaacagtct aaccggtcaa ttttttatga tttttttgat aaaaattaaa ttttatttgc
    24481 tttaatcacc accagatgac gttcgccatc cagagctgga acctgaagtt taaccactga
    24541 ttcgacctga tattcttcgg gcaacaaagc gatttcatct tccggcattt gccctttcag
    24601 cgcgtagaaa cggccttgct caccaggaag atggtggcac cagctcacca tatcgttcag
    24661 agaggcaaaa gcgcggctaa ttacgccatc aaatggcggc tctgaaggaa actcttctac
    24721 cctgctctgt actggctcaa tattctccag tttaagctca tgttgcacct gacgaaggaa
    24781 acgcacgcgt ttaccaaggc tatccaacag agtgaaatgg gcttcaggac gcacgataga
    24841 gagtggaatg cctggcagtc ctggtccggt gccgacatcg ataaaccgtt caccttgcag
    24901 atacggtgcc accacaatgc tatcgagaat atggcgtacc agcatctcat taggatcgcg
    24961 gaccgaagtc aggttgtacg ctttgttcca tttatgcagc atattcacgt aggcaataag
    25021 ctggtttttc tggtgatcgg taagcgaaat acctgcgtct ttcagcagta aggagagttt
    25081 gttgagcacg gtgattacct gttcttgatg cgttgcctgg taagcgggtg cttaccaggc
    25141 atttttaatg cgttatgcgc tacgacgcag cataccctgt tttttcagcc acaccagcag
    25201 aatggagata gccgcaggcg tgacgccaga aatacgcgaa gcctggccga tagaggccgg
    25261 tttgtgatcg ttaagtttgg cgatcacttc gttagaaaga ccggatacct ggcggtaatc
    25321 cagtgtcgcg ggtagcaggg tgttctcgtt acgcagctgc ttttcgatct catcttgctg
    25381 gcgcgcgata taaccttcgt atttaacctg aatctcaacc tgttccgccg cctgttcgtc
    25441 tgtcaacgca ggggcaaacg gcgtcagcgt ggttaatttt tcataagtca tttccggacg
    25501 acgcagcaga tcttcaccac tggcttcacg ggaaagcggc gcagtcaggt gagcattcac
    25561 ttcggctgca gcttccgccg acggggttac ccaggtcgat ttcagacgct gacgctcacg
    25621 ctcgatattc tcaagtttct cgttaaagcg cgcccaacgt tcgtcatcca ccaggcccag
    25681 ttcacgaccg atttcagtca aacgcagatc cgcattatct tcgcgcagca tcagacgata
    25741 ttctgcgcgc gaagtaaaca tacgatacgg ttctttggtt cctaaagtgc acaggtcatc
    25801 aaccagtacg ccgagatacg cctgagaacg cgccggagcc caaccttctt tgtcagcaga
    25861 cagacgggca gcgttaagac cgaccagcaa accttgcgca gcggcttctt cgtaaccggt
    25921 agtgccgtta atctgaccag caaagaacag cccctggata aacttgctct ccagcgtcgg
    25981 tttcaggtcg cgtggatcga agaagtcata ctcaatggca taacccggac gcacgatctt
    26041 cgcgttttcc atcccctgca tggagcggac gatttgcatc tgcacatcga acggcaggct
    26101 ggtggagata ccgttcggat aaatttcgtt agaggtcagc ccttccggtt caaggaagat
    26161 ctgatgctga tttctgtcgg cgaagcgcat gactttgtct tcgatcgatg ggcagtagcg
    26221 tgggccgaca ccttcgatca cccctgcgta cattgggcta cgatcgaggt tactgcggat
    26281 cacatcatgg gttttctcgt tggtatgagt gatataacac ggcacctgct ggggatgctg
    26341 ggacgcattg cccataaacg agaataccgg cattgggtta tcgccatgct gttgcgccag
    26401 cacactaaag tcgatagttc gagcatcaat acgcggtggt gtcccggttt tcagacgacc
    26461 aacgcgcagc ggcagttcac gcaaacggcg agaaagcgga atggacggcg gatcaccagc
    26521 acgaccaccg ctgtaattat ccagaccgat atgaatttta ccgtcgagga acgtcccaac
    26581 ggtgagcacg acggctttgg cacggaactt cagtcccatt tgggtaacag caccgaccac
    26641 gcgatcgttt tcgacaataa gatcttcaac cgcctgctgg aagatcatca ggttcggttg
    26701 gttctccagc gccgtacgta ccgcctgacg gtagagcaca cgatccgcct gagctcgggt
    26761 agcgcgaacc gccggtcctt tgcttgcgtt tagtatccta aactggatac ccgcttgatc
    26821 gatcgctttc gccatcagac cgccgagtgc atccacttct tttaccagat gtcccttccc
    26881 aataccgccg atcgccgggt tgcagctcat ctgccccaga gtgtcgatat tgtgtgtcaa
    26941 aagcagagtc tgttgaccca tacgcgccgc ggccatcgcg gcctcggtgc ctgcatgacc
    27001 cccgccaatg atgatgacgt caaaaggatc cggataaaac atggtgattg cctcgcataa
    27061 cgcggtatga aaatggattg aagcccgggc cgtggattct actcaacttt gtcggcttga
    27121 gaaagacctg ggatcctggg tattaaaaag aagatctatt tatttagaga tctgttctat
    27181 tgtgatctct tattaggatc gcactaccct gtggataaca aggatccggc ttttaagatc
    27241 aacaacctgg aaaggatcat taactgtgaa tgatcggtga tcctggaccg tataagctgg
    27301 gatcagaatg aggggttata cacaactcaa aaactgaaca accgttgttc cttggataac
    27361 taccggttga tccaagcttc ctgacagagt tatccacagt agatcgcacg atctgtatac
    27421 ttatttgagt aaattaatcc acgatcccag ccattcttct gccggatctt ccggaatgtc
    27481 gtgatcaaga atgttgatct tcagtgtttc gcctgtctgt tttgcaccgg aatttttgag
    27541 ttctgcctcg agtttatcga tagccccaca aaaggtgtca tattcacgac taccaatacc
    27601 gattgcgcca aagcggaccg cagaaagatc gggcttctgt tcctgcaatg cttcatagaa
    27661 aggagaaagg ttgtccggaa tatctccggc accgtgggtg gagctgataa tcagccagat
    27721 ccccgaggca gataaatctt ccaacagcgg accgtgcagc gtttcggtgg taaaacccgc
    27781 ctcttccagc ttttcagcca ggtgttctgc tacatattcg gcaccgccga gggtgctgcc
    27841 gctgataaga gtgatatctg ccataaaccg ccacctttat taagagtggc gtattgtacg
    27901 ctgtgaacgc gttgggatct acctgtggaa aagtatggga ttaaaaaagc cgattatggc
    27961 ttgatggtac gcatgatcgg gttttgcagg acgatcaatg tctcggtgga ctgaatttca
    28021 tcaattgttt ggatcttgtt gataagtaca tgctggagag cgtcgatcga acggcacatt
    28081 acttttataa agatgctgta gtggcctgtt gtgtaatagg cttcagtgac ttcagcaagg
    28141 ctttccagct ttgccagcgc ggaagggtag tctttggcgc tctttaatat aatgccgata
    28201 aagcagccta cgtcataacc gagctgcttc gggctgacat caatacgcgc cccggtaatg
    28261 atccccgcct gcttcatttt ctctactcga acgtgaatcg tccccggact gacgccaaat
    28321 tgtttcgcca gttcggcgta agcggtgcgc gcattgccca ttaatgcttc caggatgcca
    28381 cggtccagat tgtcgatcag ataattttcc ataggatttt cttatgcaga ttgatgattc
    28441 attctatttt agccttcttt tttaatgaat caaaagtgtg ttaggctttt tattgaatga
    28501 ttattgcatg tttgtcggtt tttgttgctt aatcataagc aacaggacgc aggagtataa
    28561 aaaatgaaaa ccgcttacat tgccaaacaa cgccaaatta gcttcgtgaa atctcacttt
    28621 tctcgccaac tggaagaacg tctggggctg atcgaagtcc aggcgccgat tcttagccgt
    28681 gtgggggatg gcacgcagga taacttgtcg ggctgtgaaa aagcggtgca ggtaaaagtg
    28741 aaagctctgc ctgatgccca gttcgaagtg gttcattcac tggcgaagtg gaaacgtcag
    28801 accttagggc aacacgactt cagcgcgggc gaagggctgt acacgcacat gaaagccctt
    28861 cgccccgatg aagaccgtct ttctccgttg cactcggtct atgttgacca gtgggactgg
    28921 gaacgcgtaa tgggcgacgg tgagcgtcaa ttctcgactc tgaaaagcac ggtagaggcg
    28981 atctgggcgg gaattaaagc aaccgaagct gcggttagcg aagagtttgg cctggcaccg
    29041 ttcctgccgg atcagatcca cttcgtacac agccaggagt tactgtctcg ttatccggat
    29101 ctcgatgcca aagggcgtga gcgggcgata gcgaaagatc ttggcgcggt attccttgtc
    29161 gggattggcg gcaagctgag cgatggtcat cgccacgacg tgcgcgcacc ggattatgat
    29221 gactggagca ccccgtcaga gctgggtcat gcgggtctga acggcgatat tctggtgtgg
    29281 aaccctgtac tggaagatgc gtttgagctt tcttccatgg ggatccgcgt ggatgccgac
    29341 acgttgaagc atcagttggc gctgaccggt gatgaagatc gtctgcagct ggaatggcat
    29401 caggcgctgc tgcgcggtga aatgccgcag accatcggcg gcggtatcgg ccagtctcgt
    29461 ttgaccatgc tgctgctgca actgccgcat atcggccagg ttcagtgtgg tgtatggcca
    29521 gctgctgttc gcgagaacgt cccttctctg ctgtaataat ttatcgccgc cagcgtctga
    29581 gcaggcggct tcgcatcccg gtatcaaagc gccagatatg atcgaaaatg cgcatgatgc
    29641 cgggtttgcc gtgtgccgac atcgccacgg catgaaagcg atgctgatgt acccgctgca
    29701 gctctttcac tttactcgtc acgtcgtcag gcaaccgctg agcgataaaa tcagaaatca
    29761 ccaccgcatc ggcatcaaac cattccctgc tttgcaagcg ttccataatg gcgcgaaaac
    29821 aactggcaag atcggtgccg ccacgaaact gctggcttaa aaaacggatt gcttgttcga
    29881 tgccttgggg gcctgaaagc tcataacgga cgatctcggt ggaaaatagc ataatatagc
    29941 agcgccggtt ttcagcgaga gcaatgcgca tcaaggccag gcagaacgct ttcgcacact
    30001 gttcattaaa gccgcccatt gagccggaag tatccacaca gacaataaac ggcccgcgcg
    30061 gctgttcatc gtaatcttta tgtaccaccg gacgttcgat cactttttca cgccacgact
    30121 caccgtgcag gcgataggtg agcaactgtt tttccaccag ccgacggtaa aactcatact
    30181 ccagttccgt tatccctagt gtcgccagtt ctggcggcag aagacgtaaa atatcatcgc
    30241 tttgttgcag accatcaacc tgctcaggaa ccgtcgccgg ttcgcgcacc atggtgcgga
    30301 aggtttccat ctgcgcatcg ttgcgcggta ttgatttggc ttcccgagaa cgccccagct
    30361 gctctgccag gcgtttcagt tccggctgtt cgttaagaaa ttcaccgtat ttcacaatca
    30421 actgatagtc gccacgttta agctgaccgg cgctcatatc ccacagacga ccagctgcag
    30481 tattgttatc tgcgagaatc ggttcaagtt gtccgctcag cgtcatgcgt tcttgaactt
    30541 cactcaacag ttgttcgcgt tcttcttcta atagctgttg atttaacgtc gttgcttgca
    30601 cgatcagact taaacgccaa cgctggagaa aaagcgtgtg taacgctgaa gttattgtgc
    30661 tgttagcatc aaccaactgc cgggcttgtt ctgcccatgg ggaattcaga cgatgcagta
    30721 agtccaggat ctgtggtagc tgcacaataa actgcggcgt ggagaggagc tggctttgct
    30781 gatagcacat cacctcttcg gtgagttccg gcgggactcg ggcatctttc agccgactgc
    30841 gcagcgcctc acgccagcgg ggaacatcat cagtgattgc cgccttcagg cgtgggaatt
    30901 tttcaaagaa gaccgccagc tgcggtgagg ccagcagtgc gatgatcatc tcttcgatca
    30961 atccctcttc gctgacggcc agcatcacat taagcgtatc cagcgttagc attgttgtgc
    31021 ctggtgaatc tgtgcaccga catcctgtag gctggcttca atgcgaccta accagtcgcc
    31081 agggataaac agacattttt gctgttcgct gaataaagcg tgttgcttgc gccagtcgct
    31141 ttcaagctct tccagttgtt gttttatttc accaggcaga ccttcagccg atgaaccggg
    31201 aagtgccagc gtactgcctt gtaaacttac atcgcgaacg acaagatgtt gggcgctatc
    31261 aacttccaga ttcagtttct gggcaaagcc gataccgttc agtttaccgc gaatttcacc
    31321 acctttgctc agccactgtt ccagcgcgct acgctcaaag gagatatgaa ccacttccat
    31381 atcatgcagt tttaacggtt tttgcagcag tagagtcaga gtggaagcag taacgttaac
    31441 aggaagttga tactgctgac gacggctgaa aatgccgccc agacgaatta ccgttaaggc
    31501 tgttttatcg ctttgttgct gctggagttg caggtgacgt tgcacaatcg cgccaaggcg
    31561 agtcaacatc ccttgctgtt gccaggcatg accggtcatc aatacatcaa tttgttgttg
    31621 tatcaaattc aggctttgcg cgtcatacca caggcaatct ttcagcaaaa tgagatctac
    31681 cggggcaaca gcactgcgac cgctaaaaaa ggcgctggcc tgcaataatc ggatcgcttt
    31741 tttccagcga cgatccgaga cataaggcgc atccggtaat ttatccagtt gcaggcgcag
    31801 cataaaaatc agctcaaata catgatcggg cagcgtaatt tcaccaatct ctttctgcca
    31861 gcgttcatat tcttcatctg tgacctgcaa ggcatcagga accggattgt cgttttcatc
    31921 ctgttgactg gtcagcatgg agcggaaatt cgctttatcc tgcactttat ctaaccacag
    31981 acgaatcagc atgcggtcat ataacgcttc cagactgctg tctgcttccg gcagctcgtt
    32041 ggaggccgcc accagcagac gcatcgggat tttttctacg tgtgcaccgt tgcggaactg
    32101 gcgctcgtta atggcggtga gcaaggtatt aagaattgcc gggcccgctt tccagatctc
    32161 atccagaaag acaatttctg cttccggcag gtaaccgctg gttaaacgtt catagcgccc
    32221 ttcatctttt agcgcctgaa tagaaagggg accaaaaact tcttccggcg tggagaagcg
    32281 ggtcatcaga tattcaaacg cgcgggcatt ctgaaaggcg aattttaagc gccgggcgat
    32341 caaacttttg gcaatacctg gcgggccaag gaggaacaca ctttcaccac ttaatgccgc
    32401 taataaacac aagcggatgg cgtggctacg ttcataaagc cccttttcca acgaactgct
    32461 caggcgggaa attctttc
//
LOCUS       DAJVRF010000033        32386 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJVRF010000033 DAJVRF010000000
VERSION     DAJVRF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.033"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            268..1926
                     /locus_tag="OQN13_001410"
     CDS             268..1926
                     /locus_tag="OQN13_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCS1614802.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1923..2879)
                     /locus_tag="OQN13_001411"
     CDS             complement(1923..2879)
                     /locus_tag="OQN13_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCS1614803.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3030..4145
                     /gene="macA"
                     /locus_tag="OQN13_001412"
     CDS             3030..4145
                     /gene="macA"
                     /locus_tag="OQN13_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCS1614804.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4142..6088
                     /gene="macB"
                     /locus_tag="OQN13_001413"
     CDS             4142..6088
                     /gene="macB"
                     /locus_tag="OQN13_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCS1614805.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6161..6385)
                     /gene="cspD"
                     /locus_tag="OQN13_001414"
     CDS             complement(6161..6385)
                     /gene="cspD"
                     /locus_tag="OQN13_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCS1614806.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6708..7028
                     /gene="clpS"
                     /locus_tag="OQN13_001415"
     CDS             6708..7028
                     /gene="clpS"
                     /locus_tag="OQN13_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCS1614807.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7059..9335
                     /gene="clpA"
                     /locus_tag="OQN13_001416"
     CDS             7059..9335
                     /gene="clpA"
                     /locus_tag="OQN13_001416"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCS1614808.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9741..9828)
                     /locus_tag="OQN13_001417"
     tRNA            complement(9741..9828)
                     /locus_tag="OQN13_001417"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9792..9794),aa:Ser,seq:gga)
     gene            complement(10082..10300)
                     /gene="infA"
                     /locus_tag="OQN13_001418"
     CDS             complement(10082..10300)
                     /gene="infA"
                     /locus_tag="OQN13_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCS1614809.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10585..11289)
                     /gene="aat"
                     /locus_tag="OQN13_001419"
     CDS             complement(10585..11289)
                     /gene="aat"
                     /locus_tag="OQN13_001419"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCS1614810.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(11331..13052)
                     /gene="cydC"
                     /locus_tag="OQN13_001420"
     CDS             complement(11331..13052)
                     /gene="cydC"
                     /locus_tag="OQN13_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCS1614811.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGGAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13053..14819)
                     /gene="cydD"
                     /locus_tag="OQN13_001421"
     CDS             complement(13053..14819)
                     /gene="cydD"
                     /locus_tag="OQN13_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCS1614812.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14942..15907)
                     /gene="trxB"
                     /locus_tag="OQN13_001422"
     CDS             complement(14942..15907)
                     /gene="trxB"
                     /locus_tag="OQN13_001422"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCS1614813.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16452..16946
                     /gene="lrp"
                     /locus_tag="OQN13_001423"
     CDS             16452..16946
                     /gene="lrp"
                     /locus_tag="OQN13_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCS1614814.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17081..21148
                     /gene="ftsK"
                     /locus_tag="OQN13_001424"
     CDS             17081..21148
                     /gene="ftsK"
                     /locus_tag="OQN13_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCS1614815.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21303..21914
                     /gene="lolA"
                     /locus_tag="OQN13_001425"
     CDS             21303..21914
                     /gene="lolA"
                     /locus_tag="OQN13_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCS1614816.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21925..23268
                     /gene="rarA"
                     /locus_tag="OQN13_001426"
                     /gene_synonym="mgsA"
     CDS             21925..23268
                     /gene="rarA"
                     /locus_tag="OQN13_001426"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCS1614817.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23359..24651
                     /gene="serS"
                     /locus_tag="OQN13_001427"
     CDS             23359..24651
                     /gene="serS"
                     /locus_tag="OQN13_001427"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCS1614818.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24890..27334
                     /gene="dmsA"
                     /locus_tag="OQN13_001428"
     CDS             24890..27334
                     /gene="dmsA"
                     /locus_tag="OQN13_001428"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HCS1614819.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27345..27962
                     /gene="dmsB"
                     /locus_tag="OQN13_001429"
     CDS             27345..27962
                     /gene="dmsB"
                     /locus_tag="OQN13_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCS1614820.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27964..28827
                     /gene="dmsC"
                     /locus_tag="OQN13_001430"
     CDS             27964..28827
                     /gene="dmsC"
                     /locus_tag="OQN13_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCS1614821.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28863..29489)
                     /locus_tag="OQN13_001431"
     CDS             complement(28863..29489)
                     /locus_tag="OQN13_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1614822.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29804..30952
                     /locus_tag="OQN13_001432"
     CDS             29804..30952
                     /locus_tag="OQN13_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1614823.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            31162..32386
                     /locus_tag="OQN13_001433"
                     /pseudo
     CDS             31162..32386
                     /locus_tag="OQN13_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
BASE COUNT         7949 a         7921 c         8701 g         7815 t
ORIGIN      
        1 gggttgcccc atatagtcat ttgtctgatt gacagtgtag tgcacgcaaa agatttaatc
       61 ctttaggcgt aataaaaaat aatatatcat gctaattatt tgattttgtt gtttttgcag
      121 acttatcagc aagagggagt ataacgcgat tattcgctca tttttcagac atttgccatg
      181 cttaaatgtg atgtcatcac gtattagcaa ggcctttccc gttatactgc cagcgtaaag
      241 gataagtcac atatttctgg aggggatatg attcttgagc gcgttgaaat tgtgggtttt
      301 cgcggtatca accgtttgtc gttgatgctg gaacaaaaca acgtcctgat tggggagaac
      361 gcgtggggta aatccagctt gctggacgcc ttaaccctgc tgctatcgcc agaatcagat
      421 ctctaccatt ttgagcgcga cgatttctgg ttcccgccgg gagatatcaa cgggcgagaa
      481 catcatctgc atattatttt gaccttccgc gaatcgctgc caggccgaca tcgggttcgc
      541 cgttatcggc cgctggaagc gtgctggacg ccatgcaccg atggctatca ccgtattttt
      601 tatcgtctgg aaggggagag tgcggaagac ggcagcgtga tgacactgcg cagttttctc
      661 gataaagacg gacatccgat tgatgtcgag gatattaacg atcaggcacg ccatctggtg
      721 cgtttaatgc cggtgctgcg cttgcgtgat gcccgtttta tgcgccgtat tcgtaacggc
      781 acggtgccaa atgtccctaa tatggaagtc accgcgcgcc agctcgattt cctcgcccgt
      841 gagttatcct cacatccgca aaatctctct gatgggcaga ttcgtcaggg actttccgca
      901 atggtacagc tgcttgagca ttatttctct gagcaggggg ccggacaggc gcgatatcgt
      961 ttaatgcggc ggcgagccag caatgagcaa cgaagctggc gctatctgga tatcatcaac
     1021 cggatgattg accgacctgg tgggcgctcg tatcgggtta ttttgctcgg cctgtttgct
     1081 actttgttgc aggcaaaagg cacattgcga ctggataaag acgcccgtcc attgttgctg
     1141 atcgaagatc cagaaacccg tttacacccc attatgcttt cagttgcctg gcatctgttg
     1201 aatcttctgc cattgcagcg cattgtcacc accaactcgg gtgagttgct ttcgttaacg
     1261 ccggtagagc atgtttgccg actggtacgt gagtcctcgc gcgttgccgc ctggcgtctg
     1321 gggccgagtg gcttgagtac cgaagatagc cgacgcatat cctttcacat tcgttttaac
     1381 cgtccgtcat cgctgtttgc acgctgctgg ttgctggtgg aaggggaaac ggaaacctgg
     1441 gttatcaatg aactggcgcg tcagtgcgga catcattttg atgccgaagg gatcaaggtc
     1501 attgagtttg cccagtccgg gctaaagcca ctggttaaat ttgcccgccg aatggggatt
     1561 gaatggcatg tactggtcga tggcgatgaa gcagggaaga aatatgccgc tacggtacgc
     1621 agcctgttga ataacgatcg ggaagccgaa cgagaacatt taacggcgtt accggcgctg
     1681 gatatggaac attttatgta tcgccaggga ttttccgatg tgttccaccg cgtggcgcaa
     1741 atcccggaaa atgtaccgat gaatctacgc aaaattatct cgaaagcgat ccatcgctct
     1801 tccaaacccg atcttgccat tgaagtggca atggaggcag gacgtcgtgg tgtggactcc
     1861 gtaccgacgc tgctgaaaaa aatgttctca cgcgtgctgt ggctggcgcg cggtcgcgcg
     1921 gattaaccgc gaaacatcgt ggccatttgt ggctgaatag cgtcgagcat ctcatagcgc
     1981 cgacggtatt cagcccgttt tttactggcg atttcggcaa tctcttttcg tgctatctgt
     2041 gctggaaggc ggtaatggcg ttcagcatca catacgccgc caaccgattc ccagaaagcg
     2101 ttgtaatcag cgtggatctt gccttcttta tcgcgataac gcaggctgcg gtaaatatgc
     2161 gtttcattgc tgacggcaat aatctgctct acctgcaaac gttgggcaaa cagacaggcc
     2221 gcttccatca cgaggcgttt gggaaatagc ccgtggcagg ctttcgtcgc attctggatt
     2281 tcctgatgtg gaatttccca ttttgcgcct tgcagtccgc caataaacat cgttcttttc
     2341 ccctgatatt cacacagggt aaacgtgatc tctgccagag gaataccttc gctgttgcga
     2401 aacaggattg tgctgtcacc ttctttatcc attgagatca tcatggtcag ctcaagcgtg
     2461 aactgctcgc cgtttttgcc ttccagcttc gccagttgca gcccgggggt attcaaatat
     2521 aagctgaatt cttccgccga catacatccg cggagtaacg cataatggta acgtaacgcc
     2581 tccagcaatt gcttacggct aagattcgcc gcaaggtaag ggcgatgcag acgcacaggc
     2641 agtcgcggtt ggcgcgttaa caacacattg agattaggcc agtgggaaag ttcgttcatc
     2701 cactcaacgc ttaaacgcgg cataatcaac gagcgcagca aaaatttctg gcgaaaacta
     2761 cggcgatgcc agaatttacc cggccgacac tgtccacgtg ccagactaag aaaaagtgac
     2821 aggctgctga gagattcaga tggcgtaaag gtccgttcag ttagctgcga catattcatg
     2881 aaatcaatgg ttatacatga cgtcgatttc accattgcgt atcttaacca aacatcaata
     2941 gtgtgattac taacgtaaat tttatggttt tgttgatatt tcgttgaagt taatgacccg
     3001 gattggcata tggagtattc agaatattta tgaaaaagcg gaaaaccgtg aagaagcgtt
     3061 acgttattgc gctggtgata gtcatcgccg gactgattac gttatggaga attcttaacg
     3121 cacccgtgcc gacttatcag acactgattg tgcgccccgg tgatttacag caaagcgtgc
     3181 tggcgaccgg aaagctggac gcgctgcgta aggttgacgt gggcgcgcag gtcagcggtc
     3241 agttgaaaac gctgtcggtg gcgattggcg ataaagtaaa aaaagaccag cttttagggg
     3301 tcattgatcc tgaacaggct gaaaaccaga tcaaggaggt cgaagcaacg ctgatggagc
     3361 tacgcgcgca gcggcagcag gcggaagcgg agctgaaact ggcgcgggtg acgtattccc
     3421 gtcagcaacg tctggcacaa acgcaggcgg tttcacagca ggatctcgac accgccgcga
     3481 cggagatggc tgtgaaacag gcgcaaattg gcaccattga cgcgcaaatc aagcgcaatc
     3541 aggcttctct cgatacggct aaaaccaatc tcgattacac tcgcatcgtt gccccgatgg
     3601 ccggggaagt cacgcaaatc accactctgc aaggccagac ggtgattgcc gcacaacaag
     3661 caccgaacat tctgacgctg gcagatatga gcaccatgct ggtaaaagcg caggtttctg
     3721 aagcggatgt aatccacctg aagccggggc aaaaagcctg gtttacggta cttggcgatc
     3781 cactgacgcg ctacgagggg caaatcaagg atgtactacc gacgccggaa aaggttaacg
     3841 acgctatttt ctattacgcc cgttttgaag tccccaaccc caatggtttg ctgcggctgg
     3901 atatgactgc gcaagtgcat attcagctca ccgatgtgaa aaatgtgctg acgatccctc
     3961 tgtcggcgtt aggcgatccg gttggcgata atcgttataa agtcaaattg ttgcgtaatg
     4021 gcgaaacacg cgagcgtgaa gtgacgattg gcgcacgtaa cgataccgat gttgagattg
     4081 tcaaagggct ggaagcgggc gatgaagtgg tgattggtga ggccaaacca ggagctgcac
     4141 aatgacgcct ttgctcgaat taaaggatat tcgtcgcagc tatcctgccg gtaatgagca
     4201 ggttgaggtg ctgaagggca tcagcctcga tatttatgcg ggtgagatgg tcgcgattgt
     4261 tggcgcttcg ggttccggta aatcgaccct gatgaatatt ctcggctgcc tggataaggc
     4321 caccagcggc acctatcgcg tagccggtca ggatgttgcc acgctggacg ccgatgcgct
     4381 ggcgcaactg cgccgcgagc atttcggctt tattttccag cgttaccatt tgctttcgca
     4441 tttaaccgcc gagcagaacg ttgaagtacc cgccgtctat gctggtcttg agcggaaaca
     4501 gcgactgctt cgtgcccagg agttgctgca acggctgggg ctggaagacc gtacagagta
     4561 ttatccggca cagctttcgg gtggtcagca acagcgcgtc agcatcgcgc gggcattgat
     4621 gaacggcggt caggtaattc ttgccgatga accaaccggc gcactggaca gccattctgg
     4681 cgaagaggtg atggcgatcc tgcatcagct gcgcgatcgc gggcatacgg tgattatcgt
     4741 cacccacgat ccgcaggtcg ctgctcaggc cgagcgggtg atcgaaattc gcgacggcga
     4801 aattgtgcgc aatcctcccg ccattgaaaa agtgaatgtt gctggcggga cggagcctgt
     4861 tgtcaataca gtgtctggct ggcggcagtt tgtcagcggt tttaacgagg cgctgacgat
     4921 ggcatggcgg gcgctggcag cgaataaaat gcgtacttta ctgaccatgc tggggattat
     4981 tatcggtatt gcgtcggtgg tttccattgt cgtggtgggt gacgccgcca aacaaatggt
     5041 gctggcggat attcgttcta ttggtacgaa tactattgat gtctatcccg ggaaagattt
     5101 tggcgatgac gatccgcaat atcagcaggc gctgaagtac gacgacttaa tcgccatcca
     5161 aaaacaaccg tgggtcgcct cagccacacc tgccgtctcg caaaacctgc gcctgcgtta
     5221 taacaatgtt gatgttgctg ccagtgccaa tggcgtgagc ggcgattatt ttaatgtcta
     5281 tggcatgacc ttcagtgaag gaaacacctt taatcaggag cagctgaacg gtcgtgcgca
     5341 ggtcgtggtt ctcgacagta atactcgccg ccagcttttc ccccataaag cagatgtggt
     5401 tggcgaggtg attctggtcg gcaatatgcc cgccagagtc attggtgtgg cggaagaaaa
     5461 acagtcgatg tttggtagca gtaaagtgct gcgtgtctgg ctaccttaca gcacgatgtc
     5521 cgggcgagtt atgggccagt cgtggcttaa ctccattact gtcagggtga aagaaggatt
     5581 tgacagcgcc gaggcggaac agcaactcac gcgtttactt tcactgcgcc acggtaagaa
     5641 ggatttcttt acctggaaca tggacggcgt cttgaaaact gttgaaaaga ccacacgtac
     5701 tttacaactg tttctgacgc tggtggcggt gatttcgctg gtggtgggtg gtattggtgt
     5761 aatgaatatt atgctggtgt cagtgaccga gcggacgcgg gaaattggca ttcgcatggc
     5821 tgtaggtgcg cgagcaagcg atgttttgca acagttcctg atcgaagccg tactggtttg
     5881 cctggtcggt ggcgcgttgg gaataactct gtcactgtta attgctttca ccttgcagct
     5941 tttcttaccc ggctgggaga ttggtttttc accgttggca ctgctgctgg cgtttctctg
     6001 ctcgacggcc accgggattt tatttggatg gttacccgca cgaaatgcgg cacgactgga
     6061 tccagtagat gctctggcac gagagtaatt tttgagataa aaatgccagc cgatcgggct
     6121 ggcattttgc ctttaggatg tacacaatga gacagaagag ctatgcgact gccgcttcta
     6181 cttcgacggg cacaataaca ctggcgtgat tgccttttgg cccctggtgg acatcaaact
     6241 gaacggattg tccagctttt agcgttctat aaccatccat ctgaatggtg gaataatgag
     6301 cgaaaatatc ttcgccgccg ccttcagggc agatgaaacc aaaccctttg gcattgttga
     6361 accacttaac agtacccttt tccatgcttc gacatccttc gcaaatctta tacaagtaag
     6421 atggaataaa ccggggtcag agagggggct gttcaaaacc tcgccaactc tagaaataca
     6481 atttagagaa ttagggcgag ccgtcaagca tttgacaggg gccagggggc aggtatgaat
     6541 caaaaatttg aagcagttaa cgctattgac aggaatgtga cagatgtcgc tgatgccaac
     6601 gatagatgat agttatctat catgtggagt agattggtca ggcaaataag ctcttgtcag
     6661 cggcagggcg ttctgccgat aaccgtaacc gaagatgata actgacaatg ggtaaaacga
     6721 acgactggct ggactttgat caactggcgg aagaaaaagt tcgcgacgcg ctaaaaccgc
     6781 catctatgta taaagtgata ttagtcaatg atgattacac tccgatggag tttgttattg
     6841 acgtgttaca aaaattcttt tcttatgatg tagaacgtgc aacgcaattg atgctcgctg
     6901 ttcactacca ggggaaggcc atttgcggag tctttaccgc cgaggttgca gaaaccaaag
     6961 tggcgatggt gaacaagtac gcgagggaga atgagcatcc attgctgtgt acgctagaaa
     7021 aagcctgaat gcaggcataa aaattggggg aggtgcctat gctcaatcaa gaactggaac
     7081 tcagtttaaa tatggctttc gccagagcgc gcgagcaccg tcatgagttt atgaccgtcg
     7141 agcacttgtt actggcgctg ctcagtaacc catctgcccg ggaggcgctg gaagcgtgtt
     7201 ctgtggattt ggttgcgctc cgtcaggaac tggaagcctt tattgaacaa accacacccg
     7261 ttctgcctgc cagtgaagag gagcgcgaca cacagccgac gctgagtttt cagcgtgtac
     7321 tgcaacgtgc ggtcttccat gtccagtcct ccggtcgcaa tgaggttacc ggtgcaaacg
     7381 ttctggtcgc tatctttagc gaacaggagt cgcaggcggc atatctgttg cgtaaacatg
     7441 aagtcagccg tctcgatgtg gtgaatttta tctctcatgg cacgcgtaaa gacgagccga
     7501 cacagtcttc tgatcctggc agccagccaa acagcgaaga acaagctggt ggggaggaac
     7561 gtatggagaa tttcacgacg aacctgaatc agcttgcgcg cgtgggcgga atcgacccac
     7621 tgattggtcg tgagaaggag ctggagcgtg ctattcaggt tctctgccgt cgccgtaaaa
     7681 acaacccgct gctggtgggg gaatctggtg tcggtaaaac cgcgattgcg gaaggtcttg
     7741 cctggcgaat tgttcagggc gatgtgccgg aagtgatggc tgactgtacg atttactctc
     7801 tcgatatcgg ttctctgtta gcgggcacaa aatatcgcgg cgactttgaa aaacgtttta
     7861 aagcgttgct caagcagctg gagcaggaca ctaacagcat cctgtttatt gatgagatcc
     7921 acaccattat cggtgcgggg gcagcgtctg gtggtcaggt tgatgcggct aacctaatca
     7981 aaccgttgct ctccagcggt aaaattcgtg taattggttc gacaacctat caggagttca
     8041 gcaacatttt cgagaaagac cgtgctctgg cgcgtcgctt ccagaaaatt gatattactg
     8101 aaccgtcaat cgaagaaact gttcaaatca tcaatggcct gaaaccgaag tatgaagcgc
     8161 accacgacgt gcgttatacc gcaaaagcgg tgcgtgcggc ggtagagctg gcggtgaaat
     8221 acatcaatga tcgtcatctg ccggataaag cgattgatgt tatcgacgaa gcgggcgctc
     8281 gcgcacgcct gatgccggta agcaaacgca agaaaaccgt taatgtggcg gatattgagt
     8341 ccgtggtggc ccgtattgca cgcattccag agaagagtgt ttctcagagt gaccgcgata
     8401 ccctgaaaaa cctcggcgat cgcctgaaaa tgctggtctt cggtcaggat aaagccattg
     8461 aggcgctgac tgaagccatt aagatggcgc gtgcaggttt aggtcacgaa cataaaccgg
     8521 tcggttcgtt cctgtttgcc ggtcctaccg gggtcgggaa aacagaggtg acggtacagc
     8581 tttcgaaagc gttgggcatt gagcttctgc gctttgatat gtccgagtat atggaacgcc
     8641 ataccgtcag ccgtctgatt ggtgcgcctc cgggatacgt tggttttgat cagggcggtt
     8701 tgctgaccga tgcggtaatc aagcatccac atgcggtgct gctgctggac gaaatcgaga
     8761 aagcgcatcc ggacgtgttc aatattctgt tgcaggtgat ggacaacggt acgctgaccg
     8821 ataacaacgg acgcaaagcg gacttccgta acgtggtgct ggtgatgacc accaacgccg
     8881 gggtacggga aactgagcgc aaatccattg gtcttatcca ccaggataac agcaccgatg
     8941 cgatggaaga gatcaagaag atctttacac cggaattccg taaccgtctc gacaacatta
     9001 tctggtttga tcatctgtca accgacgtga tccatcaggt ggtggataaa ttcatcgtcg
     9061 agttgcaggt tcagctggat cagaaaggtg tttctctgga agtgagccag gaagcgcgta
     9121 actggctggc cgagaaaggt tacgaccggg caatgggcgc tcgtccgatg gcgcgtgtca
     9181 tccaggacaa cctgaaaaaa ccgctcgcca acgaactgct gtttggttcg ctggtggacg
     9241 gcggtcaggt caccgtcgcg ctggataaag agaaaaatga gctgacttac ggattccaga
     9301 gtgcacaaaa gcacaaggcg gaagcagcgc attaatctaa ttgttaggta ggttggtgaa
     9361 gtccgtaatc tcgaaagagg ttacggactt tttgtttatg gggtggaacg gtgaaaaccc
     9421 tatttttgga ggtgaaggta atttgttgat aattagtgct gctggaaaag gtaaggataa
     9481 aaaagggtga cagcaggaaa atgagatggt tttgctttat taacaacgga ctaaacgtgt
     9541 agtatttgag ttcactgccg tacaggcagc ttagaaatcg acggggtgcg gtaaaacctt
     9601 tgcgaacgcg ttcactgccg tacaggcagc ttagaaattc acaggtaaca tactccaccc
     9661 gcccaccatg ttcactgccg tacagaaaga taaaatgcga aaaaaaagct cgcactttcg
     9721 tacgagctct tctttaaata tggcggtgag ggggggattc gaacccccga tacgttgccg
     9781 tatacacact ttccaggcgt gctccttcag ccactcggac acctcaccaa attgttttgc
     9841 taccaaacct catgggtggc aacggggcgc tactataggg agttggagta aaacggtcaa
     9901 gaagaatttt aatgataatt attgtttgct catactgtaa acaagttgtg cagtatatct
     9961 acatcgagac aagttacgga cttatacttc caaagtactt catacatatc acaaaataaa
    10021 aaggccggtt aaaccgacct tttactcgtt ctttctcttc gcccatcagg cggtaaaaca
    10081 atcagcgact acggaagaca atgcggcctt tgctcaggtc gtacggggtc agttcaacag
    10141 tcactttgtc gcccgtcagg atgcggatgt agtttttgcg cattttaccg gagatgtgtg
    10201 cagtaaccac gtgaccgttt tctaactcta cgcggaacat ggtattaggc aacgtttcaa
    10261 gaacggtacc ttgcatttca atattgtctt ctttggccat ctaatcctct ggggtatcac
    10321 taccgtaatt tgaaccggca agataatgcc gaagttctgt aaataagtaa agatttgcgc
    10381 gctaaatcgc aacaaacagg ttcggcacat tactccgaaa acacacggct aagccgcacc
    10441 aaaagcgcaa cgtataaggg agcggtgaga taaacgatgg gcgttacctg acgcgaaaaa
    10501 ttccttatcg gcagcggggt aatgagcata accaactctg cgaccgcaat tataacactc
    10561 tggggagaaa tgtgccgaaa acattcattt ttgtggtgaa aacaagcatc gtggcaccca
    10621 gaaattattc ggcaatcgtc cgaggcgcat ttgattgaga taattcagat aatcccgacg
    10681 gggaatttcg caggcaccaa gcgatgctgt gtgatcgtta aggacctggc agtcgataag
    10741 cttaccgcca tgactgataa attcctcaca gaataccaga agcgccgttt tagacgcatt
    10801 ttccatccgg ctgaacatgg actcgccaca aaatagcgtt ccctgggcca cgccgtacat
    10861 accgccgaca agctcatctt catgccagac ttcaatggag tgggcatgac cgagttcgtg
    10921 aaggcgatgg taggcttcga ccacgccacg cgtgatccag gttccttctt cgcgatcgct
    10981 ggcacaacct tcaatgacct gaccaaaagc gtaattcatc gtgacacgat agggcgagcg
    11041 tttatgaaat cgcttcatac tacggctgat atgcagtgat tcaggccaaa gcaccgcgcg
    11101 aggatcgggc gaccaccaga ggatgggggc gcctggagaa aaccacggaa aaataccacg
    11161 ctggtaagcc attaacaggc gcgcagggct aagatcgccc cccagagcca gcaggccgtt
    11221 aggctcacgt aatgcgcctt ccggggaagg gaaggctatt gaatggcgag aaagctgaac
    11281 caggcgcatg accgcaaaac tccacgcaag tcggatcgtt caataatagc ttacaaaccc
    11341 tgcttaaact ggtaataacg cccctgtctg gcaagcagtt ctgcgtgagt accttgctca
    11401 ataatttgcc cgttgtccat cactattatt tgttggaaac gagagagtcc gcgaagtcga
    11461 tgggtgacca ttaacaccgt tttctcacgc atcatttctg caagcaattc aaggatctgg
    11521 ctttcggttg tggcatctaa gccttcggta ggttcatcca gcaacaccag tggcgcatca
    11581 tgtaacagcg cacgggcgat agccagacgg cgcagttcac caccggagag ctggcgtccg
    11641 ccttcaccta accaactgtt gagacctgcg tcctcgagca gcttctccag accaacgcga
    11701 cgcaagatct ccgacagagc ctcatcacta ctgccaggcg aggcgagtaa cagattatca
    11761 cgcagcgtgg cgctaaacag atgcactcgc tgaggaacaa cgctgatggt ctgtcgtaga
    11821 gccgcttcat tcaggctggc tatggggcta tcgttaagca aaatctcgcc ctgttgcggg
    11881 tcccaagcgc gggtcagcag ttgtaacagt gttgatttgc cgcatccggt tcgcccgaga
    11941 atcgcaatat gttccccggc gtttacctga agagaaatcc ctttaagtgc ctgttgggat
    12001 tgctccggat aagtgaactg aacatcccgt aatgtcagcg aaacgcgatc ggcaacacga
    12061 gtttgggtat cagggaaggt gacttccggt ttttgatccg ttaaatcagt gatccgaacg
    12121 gcagaggcaa tgacttgccc cagatgctga aatgcacccg ttactggtgc cagtgcttca
    12181 aacgcggcta acgcgcagaa gacaaacagg gcaattaacg cgccgggttg agcattgccg
    12241 ccaacgccgc cagacgccat ccacagcatc aggatcaccg ctaacgcgcc aatgagcagc
    12301 attatcgctt gcgacaatgc ggtcagttca gattgacggc gttgcgcttc cagccattga
    12361 atttctgtat tctctagttg cgtgcgataa cgatcgctgg caccaaaaat ggtcagctca
    12421 gcttgccctt gcagccaggc cgtcagttgt tggcgatact gtccgcgaag atgagtcaga
    12481 ttttgcccgg tgctttttcc cgcacgataa aacagcggtg gcatcaggaa aagcgtcagt
    12541 aacataatgc cgcccagcgt aaaggcgagg gtgaaatcaa ggaaacttaa cccgactgtc
    12601 accaccataa tcaccacaaa agcgcccacc agcggcgaga taacgcgcag gtaaagatga
    12661 tcgagcgtat caacatccgc caccacgcga ttgagcaatt cgccctgacg atagcgcgcc
    12721 agtccggcag gggagagggg caacaatttg ctgaaggtgt aaatgcgcag atgctgcaac
    12781 acgcggaaag tcgcgtcgtg acttaccaga cgttcaaaat aacgcccggc agtacgggtg
    12841 attgcggcac cacgcacgcc cgcagcgggt agcatatagt tgaagctgta cagtccggca
    12901 ccccccgcaa ccgctgaggc cgagaggaac cagccggaaa gtgtcaacag accgatactg
    12961 gcgagcagcg tcacaattgc cagcacaata ccaagactta acatccattt atgacgttta
    13021 tacagtgcca gatagggtag caaagcgcgc atttaaatct cctcctgacg atgggccagt
    13081 aatgtggcga atgggccacc agccacactt aattccgcgt aacgtccttg ctcaataatc
    13141 tgaccatcct gcattaccca aatgacatcc cagtcagcaa gatcttctaa ctggtgggtg
    13201 accattaacg ttgtctggcg cagagaggcg gcattcagcg cctccattac gcgctgttca
    13261 ctgtgagcat caaggctggc agcgggttca tccaacagta atagcgaaca gggatttagt
    13321 aacgcacggg ccaccgccac gcgctgcgcc tgccccacgg aaaggcgggc agcctggtcg
    13381 ccaacaggcg tatcaacgcc ttgtggcagg agcggtagaa actcgctgac ccaggcgtta
    13441 tccagcgctg cttgtaattc atgttcgctg gcatcaggtc gcgccagtag tacgttatcc
    13501 cgtaatgttg ctgccggtaa ttgtggattt tgcccaaccc aggagagatg tttacgccat
    13561 gattctggtg ataaatcgcg taattctatc ccgttgattc gtagcgatcc ctgatatgag
    13621 agaaaaccag aaagcgcgtt caacagtgaa cttttacctg aaccgctgcg accaaccaac
    13681 acggcacgtt ggcctgctgg caaagtaaag ttcagcggtc cggccagcgt tttaccttcc
    13741 ggcgacgtga taaacagatc ctcggcctca atggtcaccg gatcggtcga tgctaattcc
    13801 acctcgccac gctgcggatg ggcgagcggg gtttccataa acgttttcag actgtcagct
    13861 gcaccaacag cctgggcttt agcatgataa aacgtaccga gatcgcgtaa tggctggaaa
    13921 aactctggcg caaggatcag ggccagaaaa cccgcagcca gcgtcacgcc agtatcgtag
    13981 tgaccaaaat ccagctcgcc gagataggaa aaaccaaagt agaccgccac cagagcaatt
    14041 gacagcgagg taaaaaattc gagaatgccg gaggataaaa atgccagccg tagcacttcc
    14101 attgtccgtt ggcggaaatc ttccgaagca gaacgaatac tttcaatttc agcttcacca
    14161 cgaccaaaaa tacgcaatgt ttccatgccg cgcaggcgat cgaggaaatg cccacttaag
    14221 cgagcaagag cgagaaagtt acgtcggtta gcatcggcag cccccattcc aaccagcgcc
    14281 ataaacaacg gaattaacgg tgcagtgccc agcagaatga gcgccgcagc ccagtttgag
    14341 gggaagattg ccaccacaat cagcaacggc accgacactg ccagcgccat ttgcggcagg
    14401 tagcgtgcat agtaatcatg catatcgtca atttgctcga gtaccagcgt cgcccagctc
    14461 cccgcaggtt taccctgaat ccacgctggc cctgcttgtt gcagacggtc gagaacctga
    14521 cggcggatgg caaagcggat atgctgcccg gcgtgataac ccacccgttc gcgtaaccag
    14581 accgcccatg cgcgcagtac aaaggtcaga accagtaacg taaagggaag cagcagggct
    14641 tcacggggaa tattctccat aatcatatgt tgcagaattc gcgccatgaa ccaggcctgg
    14701 gcaatgatca atatgccgct tacaaagccc agcagacgag aaatattcag ccaacgttgg
    14761 gagatgacgc tttgctgttt taaccagcgg gttaactctt tttgacgaga tttattcatt
    14821 gcacgcttag caggtgagtt atcagaatta tttgcagagc aatgttacaa cggggaaaaa
    14881 ataaaggcga cccatagtcg caaggtgtcg ccttctttac ttttgttact gatttgtaaa
    14941 attattttgc gtcagctaaa ccatcgaggt agcgttccgc atcaagtgct gccatgcagc
    15001 cagtaccggc cgaagtaatg gcctggcgat aaatgtgatc catcacgtcg cctgcggcaa
    15061 agacgccagg aatgctggtc tgggtggcat taccatgaat acccgactgt actttgatgt
    15121 agccgttttc cagttccagc tgcccttcga aaatcgcagt attcgggctg tgaccgatag
    15181 caacaaacag accggcaacg tcgagtgact cgatgttatc gctgttttgc gtatcgcgca
    15241 gacgaacgcc agtgacaccc atttgatcgc cggtcacttc ttccagcgta cggttggtgt
    15301 gcagaatgat gttgccgttc tccactttat ccatcagacg cttaataagg attttttccg
    15361 cgcggaaacc gtcacggcgg tgaatcagat gcacttccga agcgatgtta gacagataca
    15421 gcgcctcttc aaccgcggta ttgccgccgc cgatgaccgc aactttctgg ttgcgataga
    15481 agaaaccgtc gcaggttgca caagcagaaa ccccacggcc tttaaaggct tcttcagagg
    15541 gcaggccgag atagcgtgca gaagctccgg tggcaataat cagcgcgtcg caagtgtatt
    15601 cgccgttatc gccattcaga cggaacggac ggttttgcag atccaccttg ttgatatgat
    15661 caaaaatgat ctcagtttca aacttggtgg catgttcgtg catgcgctcc attaataacg
    15721 gaccggtcag atcgtttgga tcaccaggcc agttttccac ttccgtggtg gtggtcagtt
    15781 ggccgccttt ttccatgccg gtaatcagca caggttgcag gttggcgcgc gccgcgtaga
    15841 cagcagcggt gtatcccgcc gggcctgaac ccaggataag cagtttactg tgtttggtcg
    15901 tgcccatgag atccccatag ttgttggcag acaatgggca ggattgtagg gaatttacag
    15961 acgtaaaaaa agagtatgac gattttgtta acaatttgtg caatcggcag catcgataag
    16021 caggtcaaat tctcccgtca ttatcacctc tgctacttaa atttcccgct ttataagccg
    16081 attaaatgat gaataaacgc ccctgttaat gaatatctgg catgttgtac taaaaatcga
    16141 tgttttgctt tgacaatccc ctggtgtttt gcgaaaacat tcgaggaaga aaaaaaacag
    16201 tattcttata tgcgcataac catgcatgta aataccatgt ttaccgtgct agtgaaatct
    16261 acgtatggcg tggacagacg ccattcgtga tgtcgatagc tgccacaagg caacggtctt
    16321 ctcaccgtag acccaggcat tgcgcgccgt gaatcttcat gatttcggtc tatcgtgacg
    16381 ggtagcgact ctgaacagtg atgtttcagg ggcagacagg agtagggaag gaatacagag
    16441 agacaataat aatggtagat agcaagaagc gccttggcaa agatctcgac cgtatcgatc
    16501 gtaacattct taatgagttg caaaaggatg ggcgtatttc taacgtcgag ctttctaaac
    16561 gtgtgggact ttccccaacg ccgtgccttg agcgtgtgcg tcggctggaa agacaagggt
    16621 ttattcaggg ctatacggcg ctgcttaacc cccattatct ggatgcatca cttctggtat
    16681 tcgttgagat tactctgaat cgtggcgcac cggatgtgtt tgaacaattc aataccgctg
    16741 tacaaaaact tgaagaaatt caggagtgtc atttagtatc tggtgatttc gactacctgt
    16801 tgaaaacacg cgtgccggat atgtcagctt accgtaagtt gctgggggaa accctgctgc
    16861 gtctgcctgg cgtcaatgac acacggacat acgttgttat ggaagaagtc aagcagagta
    16921 atcgtctggt tattaagacg cgctaacacg gaacaggtgc aaaatcggcg tattttgatt
    16981 acactcctgt taatccatac agcaacagta ctggggtaac ctggtactgt tgtccgtttt
    17041 agcatcgggc aggaaaagcc tgtaacctgg agagcctttc ttgagccagg aatacactga
    17101 agacaaagaa gtcacattga caaagttaag tagcggccgc cgccttctgg aggcgttgct
    17161 gatccttatt gtcctgtttg ccgtctggtt gatggctgcc ttactaagct ttaacccttc
    17221 ggaccccagc tggtcgcaaa cggcctggca tgaacctatc cataatttag gtgggatgcc
    17281 cggtgcgtgg ttggcagata cgctgttctt tatttttggc gtgatggctt acaccattcc
    17341 cgtcattatt gtcggcggtt gttggtttgc ctggcgtcat cagtccagcg acgaatacat
    17401 tgattatttt gccgtttcgc tacgcatcat tggcgttttg gcgctcatcc ttacctcctg
    17461 tggtctggcg gcaatcaacg ctgacgatat ctggtatttt gcctccggtg gcgtcattgg
    17521 cagcttacta agcactacgc tacaaccact gctacacagt agcgggggaa ctattgcgct
    17581 gctctgcgtt tgggcagcgg gcctgacgtt gttcaccggt tggtcatggg tgaccattgc
    17641 tgaaaaactc ggcggctgga ttttaaacat tctcaccttc gccagtaatc gtacccgtcg
    17701 cgatgatacc tgggtcgatg aagatgagta tgaagacgac gaagagtatg aagatgaaaa
    17761 tcacggcaaa cagcatgaat cacgccgtgc ccgtattctt cgcggcgcgc tagcgcgtcg
    17821 taaacggttg gcggaaaaat tcattaatcc gatggggcgg caaacagacg ctgcgttgtt
    17881 ctccggtaag cggatggatg atgacgaaga gattacctac actgcacgcg gtgtggctgc
    17941 cgaccctgac gacgtcctat tttcgggcaa tcgtgcaacg cagccagaat atgacgaata
    18001 cgatccatta ttaaacggtg cgccaattac cgaacctgtc gctgtagcag ctgctgctac
    18061 cacggcggca caaagctggg ctgcgccggt tgaacctgtg actcagacgc cgcctgttgc
    18121 ctctgttgat gttccacctt cgcaacctac agtagcctgg cagcctgtac cgggtccaca
    18181 aacgggagag ccggttattg ctcctgcacc ggaaggttac ccacagcagc cacaatatgc
    18241 gcagcctgca gtgcaatata atgagccgct gcaacaacca gtacagccac agcagccgta
    18301 ttatgcacct gcagctgaac aacctgtgca acaaccttac tacgctcctg cggcggaaca
    18361 acctgttcag caaccgtatt acgcccctgc gccagaacaa ccggtggcag gtaacgcctg
    18421 gcaagccgaa gagcagcaat ccacttttgc tccacagtct acataccaga ctgagcaaac
    18481 ttatcagcaa ccagccgctc aggagccgtt gtaccaacag ccgcaacccg ttgaacagca
    18541 gcctgttgtg gagcctgaac ccgttgtaga agagacaaaa cccgcgcgtc cgccacttta
    18601 ctattttgaa gaagtggaag agaagcgagc ccgtgaacgt gaacaacttg cggcctggta
    18661 tcaaccgatt ccagaaccgg ttaaagaacc agaaccgatc aaatcttcgc tgaaagcacc
    18721 ttctgttgca gcagtacctc cagtagaaac cgctgccgct gtttctccgc tggcatctgg
    18781 cgtgaaaaaa gcgacactgg cgacgggggc tgccgcaacc gttgccgcgc cagtcttcag
    18841 tctggcaaat agcggtggac cgcgtccgca ggtcaaagag gggattggcc cgcagttgcc
    18901 acgaccgaaa cgtatccgcg tgccaactcg ccgtgaattg gcgtcttacg gtattaagct
    18961 gccctcacag cgtgcggcgg aagaaaaagc ccgtgaagcc cagcgcaatc agtacgattc
    19021 tggcgatcag tacaacgatg atgaaatcga tgcgatgcag caggatgaac tggcacgtca
    19081 gttcgcccag acacagcagc aacgctatgg cgaacagtat caacatgatg tgcccgtaaa
    19141 cgcagaagat gcagatgctg cggcagaggc tgaactggct cgtcagtttg cgcaaactca
    19201 acaacaacgt tattccggcg aacaaccggc tggggcgaat ccgttctcgc tggatgattt
    19261 tgaattttcg ccaatgaaag cgttgctgga tgatggtcca cacgaaccgt tgtttacgcc
    19321 aattgttgaa cctgtacagc agccgcaaca accggttgca ccgcagcagc agtatcagca
    19381 gccacaacag ccggttgcgc cacagcagca atatcagcag ccgcaacagc cggttgcgcc
    19441 gcagccacaa tatcagcagc cgcaacaacc ggttgcgcca cagcagcaat atcagcagcc
    19501 gcaacaacca gttgcgccgc agccgcagta tcaacagccg caacaaccgg ttgcgccgca
    19561 gcagcagtat cagcagccac aacagccagt tgcgccacaa ccgcaggata ccctgcttca
    19621 tccgctgttg atgcgtaatg gcgacagccg tccgttgcat aaaccgacga cgccgctgcc
    19681 ttctctggat ttgctgacac cgccgccgag cgaagtggag ccggtagata cctttgcgct
    19741 tgaacaaatg gctcgcctgg tggaagcgcg tctggctgat ttccgtatta aagccgatgt
    19801 tgtcaattac tctccggggc cggttatcac tcgctttgaa ttgaacctgg caccgggcgt
    19861 aaaagcggcg cgcatttcta acttgtcacg ggaccttgcc cgttcacttt cgacggtggc
    19921 ggtgcgtgtc gttgaagtta ttcctggcaa accctatgta ggtctggagt taccgaataa
    19981 aaaacgacaa accgtttatc tgcgcgaagt tttggataac gccaaattcc gcgataatcc
    20041 gtcgccatta accgtggtgc tgggtaaaga tatcgccggt gagccggtgg ttgccgatct
    20101 ggcgaaaatg ccgcacttgt tggttgcggg gactaccggt tccggtaaat ctgtcggtgt
    20161 gaacgcgatg atcctgagca tgctttataa agcacagcca gaagatgtgc gtttcatcat
    20221 gatcgacccg aaaatgctgg agctttcggt ttatgaaggc attccgcatc tgttaacgga
    20281 agtcgttact gatatgaaag atgccgccaa cgcgctgcgc tggtgtgtta acgagatgga
    20341 gcgtcgttat aaactgatgt ctgcgctggg tgtgcgtaat ctggcgggtt ataacgaaaa
    20401 aattgctgaa gccgatcgca tgatgcgtcc gattccagac ccgtactgga agccgggtga
    20461 cagtatggat gcccagcatc cggtgctgaa aaaagaacca tacattgtgg tgttggttga
    20521 cgaatttgcc gacctgatga tgacggtagg taaaaaagtg gaagagctga tagcacgtct
    20581 ggcgcaaaaa gcccgtgccg cgggtatcca cctcgtactg gcaactcagc gtccatcggt
    20641 tgatgttatt actggtctga ttaaagcgaa tattccgacc cgtatcgcct ttaccgtatc
    20701 cagtaagatt gactcacgta ccattcttga tcaggctggc gcggaatcac tgctggggat
    20761 gggggatatg ctctactctg ggccgaactc cacgttgccg gtacgtgtcc atggtgcttt
    20821 tgttcgcgat caggaagttc atgccgtggt gcaggactgg aaagcgcgtg gtcgcccaca
    20881 gtatgttgat ggcatcactt ccgacagtga aagcgaaggt ggcgcgggcg gtttcgatgg
    20941 cgctgaagaa ctggatccgt tgttcgatca ggcggtgcag tttgtcactg aaaaacgcaa
    21001 agcgtcaatt tctggcgtac agcgtcagtt ccgcattggt tataaccgtg cagcgcgtat
    21061 tatcgaacag atggaagcgc aggggattgt cagcgaacag gggcacaacg gtaatcgtga
    21121 agtgctggct ccaccgccgt ttgactaatt aatgcattgc cggataaggc gcggtagcgt
    21181 cgcatccggc actctatcaa ctgaaaattc agtattttct tctttcctca agctgattat
    21241 tagcctggaa tagagagtag agggaactcc cgatcgggag tgacgtaatt tgaggaataa
    21301 tgatgaaaaa aattgccatc acctgtgcat tactctcaag cttagtagca agcagcgttt
    21361 gggctgatgc cgcaagcgat ctgaaaagcc gcctggataa agtcagcagc ttccacgcca
    21421 gcttcacaca aaaagtgact gacggtagcg gcgcggcggt gcaggaaggt cagggcgatc
    21481 tgtgggtgaa acgtccaaac ttattcaact ggcatatgac acaacctgat gaaagcattc
    21541 tggtttctga cggtaaaaca ctgtggttct ataacccgtt cgttgagcaa gctacggcaa
    21601 cttggctgaa agatgccacc ggtaatacgc cgtttatgct gattgcccgc aaccagtcca
    21661 gcgactggca gcagtacaat atcaaacaga atggcgatga ctttgtcctg acgccgaaag
    21721 ccagcaatgg caatctgaag cagttcacca ttaacgtggg acgtgatggc acaatccatc
    21781 agtttagcgc ggtggagcag gacgatcagc gcagcagtta tcaactgaaa tcccagcaaa
    21841 atggggctgt ggatgcagcg aaatttacct tcaccccgcc gcaaggcgtc acggtagatg
    21901 atcaacgtaa gtagaggcac ctgagtgagc aatctgtcgc tcgatttttc ggataatact
    21961 tttcaacctc tggccgcgcg tatgcggcca gaaaatttag cacagtatat cggccagcaa
    22021 catttgctgg ctgcggggaa gccgttgccg cgcgctatcg aagccgggca tctgcattct
    22081 atgatcctct gggggccacc gggtaccggc aaaacaaccc tcgctgaagt gattgcccgc
    22141 tatgcgaacg ctgatgtgga acgtatttct gccgtcacct ctggcgtgaa agagattcgc
    22201 gaggcgatcg agcgcgcccg gcaaaaccgc aatgcaggtc gccgcactat tctttttgtt
    22261 gacgaagtgc accgattcaa caaaagccag caggatgcat ttctgccaca tattgaagac
    22321 ggcaccatca cttttattgg cgcaaccact gaaaacccgt cgtttgagct taattcggca
    22381 ctgctttccc gtgcccgtgt ctatctgttg aaatccctgg gtacagagga tattgagcaa
    22441 gtactaactc aggcgatgga agacaagacc cgtggttatg gtggtcagga tattgttctg
    22501 ccagatgaaa cacgacgcgc cattgctgaa ctggtgaatg gcgacgcgcg ccgggcgtta
    22561 aatacgctgg aaatgatggc ggatatggcc gaagtcaatg atagcggtaa gcgggtcctg
    22621 aagcctgaat tattgaccga aatcgccggt gagcgaagcg cccgctttga taacaaaggc
    22681 gatcgctttt acgatctcat ttccgcactg cataagtcgg tacgtggtag cgcacccgat
    22741 gcggcgctct actggtatgc gcgaattatt accgctggtg gcgatccgtt atatgtcgcg
    22801 cgtcgttgcc tggcaattgc ttccgaagat gtcggcaatg ccgacccacg ggcgatgcag
    22861 gtggcaattg cggcctggga ttgctttact cgcgttggcc cggcggaagg tgaacgcgcc
    22921 attgctcagg cgattgttta cctggcctgc gcgccaaaaa gcaacgctgt ctacactgcg
    22981 tttaaagctg cgctggcgga tgctcgcgaa cgcccggatt atgacgtgcc ggttcatttg
    23041 cgtaatgcgc cgacgaaatt aatgaaggaa atgggctacg ggcaggaata tcgttacgct
    23101 catgatgaag caaacgctta tgctgccggt gaggtttact tcccgccgga aatagcacaa
    23161 acacgctatt atttcccgac aaacaggggc cttgaaggca agattggcga aaagctcgcc
    23221 tggctggctg aacaggatca aaatagcccc ataaaacgct accgttaatg ttatcgttgc
    23281 ggtaatgttg ttactgtatc cctgtggtcg caggctgtgg ccacatctcc catttaattc
    23341 gataagcaca ggataagcat gctcgatccc aatctgctgc gtaatgagcc agacgcagtc
    23401 gctgaaaaac tggcacgccg gggctttaag ctggatgtag ataagctggg cgctcttgaa
    23461 gagcgtcgta aagtattgca ggtcaaaacg gaaaacctgc aagcggagcg taactcccga
    23521 tcgaaatcca ttggccaggc gaaagcgcgc ggggaagata tcgagccttt acgtctggaa
    23581 gtgaacaaac tgggcgaaga gctggatgca gcaaaagccg agctggatgc tttacaggct
    23641 gaaattcgcg atatcgcgct gaccatccct aacctgcctg cagatgaagt gccggtaggt
    23701 aaagacgaaa atgacaacgt tgaagtcagc cgctggggta ccccgcgtga gtttgacttt
    23761 gaagttcgtg accacgtgac gctgggtgaa atgcactctg gcctcgactt tgcagctgca
    23821 gttaagctga ctggttcccg ctttgtggta atgaaagggc agattgctcg catgcaccgc
    23881 gcactgtcgc agtttatgct ggatctgcat accgaacagc atggctacag tgagaactat
    23941 gttccgtacc tggttaacca ggacacgctg tacggtacgg gtcagctgcc gaaatttgct
    24001 ggcgatctgt tccatactcg tccgctggaa gaagaagcag acaccagtaa ctatgcgctg
    24061 atcccaacgg cagaagttcc gctgactaac ctggtacgcg gtgaaatcat cgatgaagat
    24121 gatctgccaa ttaagatgac cgcccacacc ccatgcttcc gttctgaagc cggttcatat
    24181 ggtcgtgaca cccgtggtct gatccgtatg caccagttcg acaaagttga aatggtgcag
    24241 atcgtgcgcc cggaagactc aatggcggcg ctggaagaga tgactggtca tgcagaaaaa
    24301 gtcctgcagt tgctgggcct gccgtaccgt aaaatcatcc tttgcactgg cgacatgggc
    24361 tttggcgctt gcaaaactta cgacctggaa gtatggatcc cggcacagaa cacctaccgt
    24421 gagatctctt cctgctccaa cgtttgggat ttccaggcac gtcgtatgca ggcacgttgc
    24481 cgcagcaagt cggacaagaa aacccgtctg gttcataccc tgaacggttc tggtctggct
    24541 gttggtcgta cgctggttgc agtaatggaa aactatcagc aggctgatgg tcgtattgaa
    24601 gtaccagaag ttctgcgtcc gtatatgaac ggactggaat atattggcta atacccaatt
    24661 tttctgaatc taaaaagcgc ctgcgggcgc tttttttgtc tccctttgat accgaacaat
    24721 aattactctt cacttacacg taatactact ttcgagtgaa aatctaccta tctctttgat
    24781 tttcaaatta ttcgatgtat acaagcctat atagcgaact gctatagaaa taattacaca
    24841 atacggtttg ttactggaat caatcgtgag caagcttgag tgagccatta tgaaaacgaa
    24901 aatccctgat gcggtattgg ctgctgaggt gagtcgccgt ggtttggtaa aaacgacagc
    24961 gatcggcggc ctggcaatgg ccagcagcgc attaacatta ccttttagtc ggattgcgca
    25021 cgctgtcgat agcgccattc caacaaaatc agacgaaaag gttatctgga gcgcctgtac
    25081 agttaactgt ggtagtcgct gcccgctacg tatgcacgtc gtggacggtg aaatcaaata
    25141 tgtcgaaacg gacaataccg gcgatgacaa ttacgacggc ctgcaccagg ttcgcgcctg
    25201 cctgcgtggg cgttccatgc gtcgccgtgt ctacaatccg gaccgcctga aatatccgat
    25261 gaaacgagtc ggggcgcgcg gtgaaggcaa attcgagcgc attagctggg aagaagccta
    25321 cgacatcatc gcgaccaata tgcagcgcct gatcaaagag tacggcaacg agtctatcta
    25381 tctgaactat ggcaccggta cgctgggcgg caccatgacc cgctcctggc cgccgggaaa
    25441 taccctggtc gcgcggctga tgaactgctg cggcggctat ctgaaccatt acggcgacta
    25501 ctcctccgcg caaattgcgg aaggtttgaa ctatacctac ggcggctggg cagatggcaa
    25561 cagcccgtcg gatatcgaaa acagtaagct ggtagtgctg tttggtaata accctggcga
    25621 aacgcgaatg agtggcggtg gggtgactta ctatcttgaa caggcacgcc agaaatctaa
    25681 tgcccgcatg atcatcatcg atccgcgcta taccgacacc ggtgccgggc gcgaagatga
    25741 gtggatccct attcgtccgg gaacagatgc cgcactggtt aacggtctgg cgtacgtcat
    25801 gatcactgaa aacctggtgg atcaggcatt cctcgataaa tattgcgttg gctacgatga
    25861 gaaaaccctg ccagccagtg cgccgaaaaa tggccactat aaagcttata ttctgggtga
    25921 agggccagat ggcgtggcta aaacaccgga atgggcctca caaatcaccg gtgttccggc
    25981 agacaaaatc atcaaactgg ctcgtgaaat cggcagtacc aaaccggcgt ttatcagcca
    26041 gggatggggc ccgcagcgtc acgctaacgg tgaaatcgca acccgtgcta tctcgatgct
    26101 ggcgattctg accggtaacg ttggtattaa tggagggaac agcggcgcgc gtgaaggttc
    26161 atacagctta ccgtttgtcc gtatgccgac cttggaaaac ccgatccaga ccagcatttc
    26221 gatgtttatg tggaccgatg ccattgaacg tggcccggaa atgacggcgc tgcgtgatgg
    26281 tgtacgcggg aaagataagc tggatgtgcc gatcaaaatg atctggaact atgccggtaa
    26341 ctgcctggtt aaccagcatt ctgaaatcaa ccgtacccat gaaatccttc aggatgataa
    26401 gaagtgcgag ctgattgtgg ttatcgactg ccacatgacc tcatcggcga aatatgctga
    26461 catcctgctg cctgactgca ccgcttccga acagatggac tttgcgctgg atgcatcctg
    26521 cgggaatatg tcttacgtga ttttcaacga tcaggtgatt aaaccgcgct ttgaatgtaa
    26581 gaccatctat gaaatgacca gcgaactggc aaaacgtctt ggcgttgagc aacagtttac
    26641 tgaaggccgt acccaggaag agtggatgcg gcatctgtat gcccagtcgc gggaagcgat
    26701 tcctgaactg ccaacgtttg aagagttccg caagcagggg atctttaaaa agcgcgaccc
    26761 acaagggcat cacgttgctt ataaagcctt ccgtgaagat ccgcaggcaa acccactgac
    26821 tacgccatcg ggcaaaattg agatttattc gcaggcgctg gctgacattg ccgctacctg
    26881 ggaattgcca gaaggcgatg tgatcgatcc actgccgatc tacacgccgg gctttgaaag
    26941 ttatcaggat ccgctgaaca aacagtatcc gctgcagctt acgggtttcc actataaatc
    27001 tcgcgttcac tcaacttacg gcaacgttga tgtgctgaaa gcagcttgcc gtcaggaaat
    27061 gtggatcaac ccgcttgatg cgcaaaaacg cggtatcaac aacggcgata aagtcaggat
    27121 ctttaacgat cgtggtgaag ttcatattga ggcgaaagtg acgccacgaa tgatgccagg
    27181 tgtggtcgca ctgggtgaag gtgcctggta tgacccggat gcaaaacgtg tcgataaggg
    27241 tggttgtatt aacgtactga ccactcaacg tccgtctcct ctcgctaagg ggaatccgtc
    27301 acatacaaac cttgttcagg ttgaaaaggt gtaaggagta accgatgaca acccagtatg
    27361 gattttttat tgattccagc cgttgcaccg gttgcaaaac ctgcgagctg gcttgtaaag
    27421 actacaaaga tttgacgcca gaagtcagct tccgccgcat ttatgaatat gctggcggcg
    27481 actggcagga agataacggt gtctggcacc agaacgtgtt tgcctactat ctgtcgattt
    27541 cgtgcaacca ctgcgaagat ccggcttgta ctaaagtctg cccgagcggt gcgatgcata
    27601 aacgtgaaga tggttttgtt gtggtcgatg aagatgtgtg cattggctgc cgctactgcc
    27661 atatggcttg cccgtatggc gcaccgcaat ataacgaaac gaaaggccat atgaccaaat
    27721 gcgatggttg ttatgaccgt gttgcggagg gtaaaaagcc gatctgtgtt gaatcttgtc
    27781 cgctgcgggc gctggatttc ggccctatcg acgagctgcg taaaaaacat ggcgatctgg
    27841 cggccgttgc gccgttgccg cgagctcact ttaccaaacc gaatattgtg atcaaaccca
    27901 atgccaatag ccgcccgacc ggggatacca ctggctatct ggcaaacccg aaggaggtgt
    27961 gagatgggaa gtggatggca tgaatggccg ctgatgatct tcacggtctt cgggcaatgt
    28021 gtggcgggtg gttttatcgt tctggctttg gcgctgctca aaggcgacct gcgagcagaa
    28081 gcccagcagc gtgttatcgc ctgcatgttt ggcttatggg tgctgatggg cattggcttt
    28141 atcgcttcta tgctccatct tggttcaccg atgcgcgctt ttaactcgct caaccgggta
    28201 ggggcttcag cactcagtaa cgaaatcgcc agcggttcga tcttttttgc cgtaggcggc
    28261 atcggctggt tgctggcaat gctgaaaaag ctgtcaccgg cattgcgtac gctttggctg
    28321 gtggtgacga tggttcttgg cgtcgtcttt gtctggatga tggtgcgtgt gtataacagc
    28381 attgataccg ttccgacctg gtacagcatc tggacgccga tgggcttctt cctgacgatg
    28441 ttcatgggcg gcccgttact ggggtacctg ttgctgagtc tggctggcgt cgatagctgg
    28501 gcgatgcgtc tgctgccagc catttccgta ctggcactgg tggtgagtgg cgtggtgtca
    28561 gtgatgcagg gcgcagagct ggcgactatt catagttctg tgcagcaggc cgcagcgctg
    28621 gtgccggact atggtgcgct gatgtcctgg cggatcgtgc ttttggccgt tgccctgtgc
    28681 ttgtggattg caccacagct aaaaggttat cagcctgcgg ttccgctact atccgtctca
    28741 ttcattctgc ttctggcagg ggaattaatc ggtcgcggcg tattctatgg tttgcatatg
    28801 accgtgggga tggccgtcgc aagctaatca gtaacaaccg gggtttcggc cccggttctc
    28861 ttttatttct gcttcgttaa cgtgtcataa ctggtcatca aattacgata atcaggaata
    28921 tggttagaga acaacgtcgc cagtccttca atatcattac gccagtcacg gtgcagctcg
    28981 caagccacgc cgaaccatgt catcaattgc gcacctgctt gcgacatgcg atcccatgcc
    29041 gaatgccggg taatttcatt aaaggtccct gacgcgtcag tcacaacaaa cacatcaaac
    29101 ccttcttcaa tggctgaaag cgccgggaat gccacgcaaa cttcggttac cacaccggca
    29161 ataattaact gttttttacc tgtcgctttg actgctttta caaaatcttc gttatcccag
    29221 gcgttaatat tgcccgggcg agcaatgtaa ggtgcatcgg ggaattgtgc tttcagttct
    29281 ggaactaatg ggccgttagg accggtttca aaactggtgg taagaatagt tggtaagttg
    29341 aaatacttag ccaggtcacc cagcgccagc acgttatttt taaacttatc gggttcgata
    29401 tcccgtacaa gggaaagtaa accagcctgg tgatcaacaa gcaaaacagc agcatcattt
    29461 ttatcaagac gaacatacgg tttggtcatc ctcttctcct ttcgagagtc agtggtacgc
    29521 ctgaaatcgg tcagacgtga tgcattgctc tgaaagcata gacgggaaat atgagtttgc
    29581 tgtgaccatg aaatttttcg actgaacgaa gcgtatgaaa tttgtgttag atcaataaaa
    29641 acaatcagat gagattaatc atctcttcag tattagcgtt atgaatgagt aatataaatc
    29701 gataataccg ccgttgcgat atgcgcgtat tttcacccat tgacaatgtt tttggcggtg
    29761 gcatgatgcg catgaaattt gaacttcctc acggttttaa ttcatgtcca cgtatacccg
    29821 gcctgtcatg cttttgctgt ctggcctgct tttgttgact ctggcgatcg cggtgttaaa
    29881 tacactcgtg ccgctttggc tcgcccagga acacatgtcc acatggcagg taggcgttgt
    29941 cagctcatcc tattttaccg gcaaccttgt cggtacattg ctgacagggt atgtcattaa
    30001 acgcattggt tttaaccgca gctattatct ggcctccttc atttttgccg ctggctgtgc
    30061 cggccttggc ctgatgattg gattctggag ttggttggcg tggcgttttg tcgcgggcgt
    30121 cggctgtgcc atgatttggg tagtcgttga gagcgcgctg atgtgcagtg ggacgtcacg
    30181 taaccgtggg cgtttgcttg ctgcatacat gatggtttat tacgtgggaa cgtttttagg
    30241 ccagttactg gtcagcaagg tctcaaccga gctgatgagc gtattgccgt gggttacagg
    30301 tttgacattg gcagggatct taccgctgtt gtttacgcgt gtcctgaatc agcaggctga
    30361 aaaccatgat tcgacgtcaa ttacggcaat gctaaaactc cgtcaggcgc ggcttggcgt
    30421 gaatggctgc attatctcag gaatcgttct gggatctcta tatggcctga tgcctctgta
    30481 cctcaatcac aaaggggtga gcaatgccag tattggtttc tggatggcgg tactggtcag
    30541 tgcgggtatc ctcggacaat ggccgattgg acgtctggcg gataagtttg gtcgactgct
    30601 ggttttgcgt gttcaggtct ttgtcgtcat tctcggcagt attgcgatgc ttagccaggc
    30661 ggcgatggcc ccagcgttat tcatcctcgg tgccgctggc tttacgctat atccggtggc
    30721 gatggcatgg gcttgcgaga aagttgaaca tcatcaactg gtggcgatga accaagcctt
    30781 actgttgagc tatactgtgg gaagtctgct tggcccgtca tttaccgcta tgctaatgca
    30841 gaatttctcc gataatttat tgtttatcat gatcgccagc gtatcgttta tctatttgct
    30901 gatgctgctg cgcaacgccg gtcatacgcc gaaacccgtt gctcacgtgt aaatgaattc
    30961 aagcagagtg tgaacttact gtttcacact ctgctttttt gtttcttcta tctgacttgc
    31021 tttattccaa attttattcg tttaaaaata aaatgtgtag caggttataa ttttgcattt
    31081 cgctatttcc gcacttctta tttgccgcgc ataatccctc gttttacaga tgcccctata
    31141 attttggcga aggatttgtc tatggctggg aatgttcagg aaaaacagct gcgatggtac
    31201 aacattgcgc tgatgtcttt tatcactgtc tggggttttg gcaacgttgt taataactac
    31261 gccaaccagg ggctggtggt tgttttttca tgggtgttta tctttgcgct ctatttcaca
    31321 ccttatgcgc taattgttgg tcagttaggc tcgaccttca aagatgggaa gggcggggtc
    31381 agtacctgga ttaaacacac gatgggaccc ggactggctt atctcgccgc gtggacctac
    31441 tgggtggtgc atattcccta tctggcacaa aaaccctagg caattctgat tgcgctcggt
    31501 tgggcgatga aaggcgacgg ttcgctaatc aaagaatatt cagtcgtagc gttacagggg
    31561 ttaacgctgg tgctgtttat cttctttatg tgggttgctt cacgcggtat gaaatcgctg
    31621 aaaatcgtcg gttctgtggc agggattgct atgtttgtta tgtcgctcct gtatgtggcg
    31681 atggcggtaa ccgcgcctgc aattactgaa gtgcatattg cgaccacaaa cattacctgg
    31741 gaaacgttca ttcctcatat cgactttacc tacattacca ctatttcaat gctggttttc
    31801 gcggttggcg gagcagagaa gatttctcct tacgttaatc aaacgcgcaa cccaggaaaa
    31861 gaatttccaa aagggatgtt atgcctggcg gtgatggttg cggtttgtgc cattctgggc
    31921 tcgctggcga tggggatgat gtttgattcg cgtaatatcc cggatgactt aatgaccaac
    31981 ggtcagtatt acgcctttca gaagctgggc gagtattaca acatgggtaa tactttaatg
    32041 gtgatttacg ccattgcgaa taccctggga caagtagcgg cgctggtatt ctcgattgat
    32101 gccccgctta aagtgctatt aggtgatgct gacagcaaat atattccagc cagtttatgt
    32161 cgtaccaacg cttctggtac gcccgttaat ggctattttc tgaccctggt actggtggcg
    32221 attctgatta tgctgccgac tctcggcatt ggtgatatga acaatctcta taaatggctg
    32281 ttgaacctta attcggtagt gatgccgctg cgttatttat gggtatttgt tgcatttatt
    32341 gcagtcgttc gcctggcgca gaaatataaa ccagaatatg tcttta
//
LOCUS       DAJVRF010000034        32207 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJVRF010000034 DAJVRF010000000
VERSION     DAJVRF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32207
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.034"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..94
                     /locus_tag="OQN13_001434"
     CDS             <1..94
                     /locus_tag="OQN13_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1614824.1"
                     /translation="SLLAKEKPVEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(136..1212)
                     /gene="glpQ"
                     /locus_tag="OQN13_001435"
     CDS             complement(136..1212)
                     /gene="glpQ"
                     /locus_tag="OQN13_001435"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCS1614825.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1217..2575)
                     /gene="glpT"
                     /locus_tag="OQN13_001436"
     CDS             complement(1217..2575)
                     /gene="glpT"
                     /locus_tag="OQN13_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCS1614826.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2848..4476
                     /gene="glpA"
                     /locus_tag="OQN13_001437"
     CDS             2848..4476
                     /gene="glpA"
                     /locus_tag="OQN13_001437"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCS1614827.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4466..5725
                     /gene="glpB"
                     /locus_tag="OQN13_001438"
     CDS             4466..5725
                     /gene="glpB"
                     /locus_tag="OQN13_001438"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCS1614828.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5722..6912
                     /gene="glpC"
                     /locus_tag="OQN13_001439"
     CDS             5722..6912
                     /gene="glpC"
                     /locus_tag="OQN13_001439"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCS1614829.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7105..8007
                     /locus_tag="OQN13_001440"
     CDS             7105..8007
                     /locus_tag="OQN13_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCS1614830.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQKGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8048..8851)
                     /gene="yfaU"
                     /locus_tag="OQN13_001441"
     CDS             complement(8048..8851)
                     /gene="yfaU"
                     /locus_tag="OQN13_001441"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HCS1614831.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8869..10158)
                     /locus_tag="OQN13_001442"
     CDS             complement(8869..10158)
                     /locus_tag="OQN13_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1614832.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(10215..11419)
                     /gene="rhmD"
                     /locus_tag="OQN13_001443"
                     /pseudo
     CDS             complement(10215..11419)
                     /gene="rhmD"
                     /locus_tag="OQN13_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            complement(11434..12216)
                     /locus_tag="OQN13_001444"
     CDS             complement(11434..12216)
                     /locus_tag="OQN13_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCS1614833.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(12436..13638)
                     /locus_tag="OQN13_001445"
     CDS             complement(12436..13638)
                     /locus_tag="OQN13_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCS1614834.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(13738..14280)
                     /locus_tag="OQN13_001446"
     CDS             complement(13738..14280)
                     /locus_tag="OQN13_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614835.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            14559..14984
                     /gene="nudI"
                     /locus_tag="OQN13_001447"
     CDS             14559..14984
                     /gene="nudI"
                     /locus_tag="OQN13_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCS1614836.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(15023..15625)
                     /locus_tag="OQN13_001448"
     CDS             complement(15023..15625)
                     /locus_tag="OQN13_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCS1614837.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            15933..17072
                     /gene="arnB"
                     /locus_tag="OQN13_001449"
     CDS             15933..17072
                     /gene="arnB"
                     /locus_tag="OQN13_001449"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCS1614838.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTHRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            17076..18044
                     /gene="arnC"
                     /locus_tag="OQN13_001450"
     CDS             17076..18044
                     /gene="arnC"
                     /locus_tag="OQN13_001450"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCS1614839.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            18044..20026
                     /gene="arnA"
                     /locus_tag="OQN13_001451"
     CDS             18044..20026
                     /gene="arnA"
                     /locus_tag="OQN13_001451"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCS1614840.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            20023..20913
                     /locus_tag="OQN13_001452"
     CDS             20023..20913
                     /locus_tag="OQN13_001452"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCS1614841.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            20913..22565
                     /gene="arnT"
                     /locus_tag="OQN13_001453"
     CDS             20913..22565
                     /gene="arnT"
                     /locus_tag="OQN13_001453"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCS1614842.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            22562..22897
                     /gene="arnE"
                     /locus_tag="OQN13_001454"
     CDS             22562..22897
                     /gene="arnE"
                     /locus_tag="OQN13_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCS1614843.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            22897..23283
                     /gene="arnF"
                     /locus_tag="OQN13_001455"
     CDS             22897..23283
                     /gene="arnF"
                     /locus_tag="OQN13_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCS1614844.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(23277..23543)
                     /gene="pmrD"
                     /locus_tag="OQN13_001456"
     CDS             complement(23277..23543)
                     /gene="pmrD"
                     /locus_tag="OQN13_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCS1614845.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(23587..25008)
                     /gene="menE"
                     /locus_tag="OQN13_001457"
     CDS             complement(23587..25008)
                     /gene="menE"
                     /locus_tag="OQN13_001457"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCS1614846.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     DYEIISFTSQFCHSIAVDNRFIC"
     gene            complement(25005..25967)
                     /gene="menC"
                     /locus_tag="OQN13_001458"
     CDS             complement(25005..25967)
                     /gene="menC"
                     /locus_tag="OQN13_001458"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCS1614847.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(25967..26824)
                     /gene="menB"
                     /locus_tag="OQN13_001459"
     CDS             complement(25967..26824)
                     /gene="menB"
                     /locus_tag="OQN13_001459"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCS1614848.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(26839..27597)
                     /gene="menH"
                     /locus_tag="OQN13_001460"
     CDS             complement(26839..27597)
                     /gene="menH"
                     /locus_tag="OQN13_001460"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCS1614849.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(27594..29264)
                     /gene="menD"
                     /locus_tag="OQN13_001461"
     CDS             complement(27594..29264)
                     /gene="menD"
                     /locus_tag="OQN13_001461"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCS1614850.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(29353..30648)
                     /gene="menF"
                     /locus_tag="OQN13_001462"
     CDS             complement(29353..30648)
                     /gene="menF"
                     /locus_tag="OQN13_001462"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCS1614851.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(30727..31032)
                     /gene="elaB"
                     /locus_tag="OQN13_001463"
     CDS             complement(30727..31032)
                     /gene="elaB"
                     /locus_tag="OQN13_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCS1614852.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(31087..31548)
                     /locus_tag="OQN13_001464"
                     /pseudo
     CDS             complement(31087..31548)
                     /locus_tag="OQN13_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            31613..>32207
                     /gene="rnz"
                     /locus_tag="OQN13_001465"
     CDS             31613..>32207
                     /gene="rnz"
                     /locus_tag="OQN13_001465"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCS1614853.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYL"
BASE COUNT         7819 a         8715 c         8054 g         7619 t
ORIGIN      
        1 ttcgttgttg gctaaagaaa agccggtaga aggcagtcca ctgtttttac tacaaatgtg
       61 catggaacaa ttagtggcga ttggaaaaat ttgatatgag acaggatggc gcatgaacgc
      121 catcctgcag tagcattact ctttattaag gaattttact gccttatcag ggaaatcagt
      181 aaacagccca tttacacccg ctttgttata cagagcatca tataactgat tcacatcagt
      241 agtgtattca ggcagtttat ccgaccgcac ggtataagga tgcactacca gtttattctg
      301 ctgagcatct tgcaccatgc cagtgagttt gatattaccc ggctgcgatg tttcctcaat
      361 caacatatgg taatccggcc cgataccgtc ggcatattcc gcgacttgct tcatggcacc
      421 tggcttaaac atccagtcat agctgtaatt aacccagctt ccgtccggct gtttctgctg
      481 cgtttcattc cagtcggtat aggcaatcag ctgtaccaga ttgagatcca tgcccatttt
      541 aggttccagc tcattcttaa tacgcttcag ctcatcagca tcaaaacatt gcaaataaac
      601 tttatcgtcc ttaccggtgt aaccatattt cttcagcact tccagcgttt ttgccgcaat
      661 atccttccct tcctgatgat ggaaccacgg cgctttgatt tctggataga taccgatatt
      721 tttcccggta gagtgattta acccctgaac aaattcaatc tcttcttcaa aggtgtgccc
      781 ccggaagtcg gacttaccca ttgggaaacg ccccggataa gtctgtactt ttttaccgtt
      841 ttcaatatcg aaaccttcgg taaatttcag cgacttaatt tcatccagcg tgaaatctat
      901 cgcgtagtaa cgaccgtctt tgcgcgcccg atccgggaaa cgatcggcaa catcagtaac
      961 acgatcgaga taatggtcat gcagaacaac cagatggtcg tctttggtca tcaccaaatc
     1021 ctgttccaga taatccgctc cctgcgcata cgccatcgct tttgctggca gcgtatgctc
     1081 cggcaaatat ccactggcac cgcgatgggc gatgactatt ttttcgttgc tgtccgccgc
     1141 cattgcactg cttcccatga ctatagtgct catcatgatc gccatgctaa ggtttttcag
     1201 cttcaatttc atgccattag cctccgttgc gtttttgcag taattgttca tggcgacgtt
     1261 tttcgccaat catcacaaca atcaacaaga taaccgccag aatgctgccg ccaatcatta
     1321 ccataaagcc gccatcccag ccgaagaagt ccacggtgta gccaacaatc gcgctcgccg
     1381 ccaccgaacc gcccaggtaa ccaaacagcc cggtaaagcc cgctgccgta cctgccgctt
     1441 ttttcggtgc cagttccagc gcatgcagac cgatcagcat cacaggaccg tagatcagga
     1501 agccgataac aatcatacaa atcatatcga cggttgggtt acctgccggg ttcatccagt
     1561 aaacgatagt cgcgatggtc accagtgtca taaagaaaac gccggttgcc ccacggttgc
     1621 cacggaagac tttatccgac atccagccgc acagcagagt gcccggaata cctgcatatt
     1681 cataaaggaa gtaggcccag gaggatttat ccagcgcgaa atgcttaacc tctttcagat
     1741 aagtcggtga ccagtcgagg atgccgtaac gcagcagata aacgaacacg ttggcgatgg
     1801 cgatatacca cagcagtttg ttcggcagta cgtactgcat gaagatttgc ttcgccgtca
     1861 gctcctgttc cgctttttcg ttatagtcgt ccggataatc atttttgtac tcttcgatcg
     1921 gcggcaagcc acaggattgc ggggtatcgc gcatcatcgc aaaggcgaat aatgccacca
     1981 gaatggcgca gaaagcaggc atatagagcg ccgcatgcca gtcattgaac caggccatcc
     2041 ccagcaggaa cagcagcggc ggaataccac caccgacgtt gtgcgcacag ttccacactg
     2101 acacaatgcc gccacgttct ttctgcgacc accagtgcac catagtacga ccacacggcg
     2161 gccaccccat cccctggaac caaccgcaga ggaacaacag tacaaacatc accgcaatgc
     2221 tcgacgtcgc ccatggcaca aagcccataa acaacatcac tgccgccgcc agaatcaaac
     2281 ctgcgggcag gaaaacgcgc ggattcgagc gatccgatac cgaacccatg atgaatttcg
     2341 aaaatccata agcaatcgag atccccgaaa gggcaaaacc taaatcaccg cgtgagaatc
     2401 cctgctcaac cagataaggc atagcaagcg caaagttctt acgaaccaaa tagtaagccg
     2461 catagccaaa gaatatcccc aggaaaattt gccagcgcaa tcgacgataa gtcggatcga
     2521 tctccgcggc aggtaagcgc gctttgtgtg gcgctggttt aaaaatactc aacattgata
     2581 gcctccgtgg cccgtggtct tatttatgat taacagcctg attcagtgag agaacctgcc
     2641 gtttcttgag ttgccgcgat gttaagaaaa cattcataaa ttaaatgtga attgccgcac
     2701 acattattaa ataagattta caaaatgttc aaaatgacgc atgaaatcac gcttcacttt
     2761 cgaatcatga gcgaatatgc gcgaaatcaa acaattcatg tttttactat ggctaaatgg
     2821 taaaaaacga acttcagagg gataacaatg aaaactcgcg actcgcaatc aagtgacgtg
     2881 attatcattg gcggcggcgc aacgggagcc gggattgccc gcgactgtgc cctgcgcggg
     2941 ctgcgcgtga ttttggttga gcgccacgac atcgcaaccg gtgccaccgg gcgtaaccac
     3001 ggcctgctac acagcggtgc gcgctatgcg gtaaccgatg cggaatcggc ccgcgaatgc
     3061 attagtgaaa accagatcct gaaacgcatt gcacgtcact gcgttgaacc aaccaacggc
     3121 ctgtttatta ccctgccgga agatgacctc tccttccagg ccacttttat tcgcgcctgc
     3181 gaagaagcag ggatcagcgc agaagctata gacccgcagc aagcgcgcat tatcgaacct
     3241 gccgttaacc cggcattgat tggcgcggtg aaagttccgg atggcaccgt tgatccattt
     3301 cgtctgaccg cagcaaacat gctggatgcc aaagaacacg gtgccgttat ccttaccgct
     3361 catgaagtca cgggactgat tcgtgaaggc gcgacggtgt gcggtgttcg tgtacgtaac
     3421 catctcaccg gcgaaactca ggcccttcat gcgcctgtcg tggttaatgc cgccgggatc
     3481 tgggggcaac acattgccga atatgccgat ctgcgcattc gcatgttccc ggcgaaagga
     3541 tcgctgctga tcatggatca ccgcattaac cagcatgtga tcaaccgctg ccgtaaacct
     3601 tccgacgccg atattctggt gcctggcgat accatttcgc tgattggcac cacctcttta
     3661 cgtattgatt acaacgagat tgacgataat cgagtgacgg cagaagaggt tgatattctg
     3721 ctgcgtgaag gggaaaaact ggcccccgtg atggcgaaaa cgcgcatttt gcgggcctat
     3781 tctggcgtgc gcccgctggt tgccagcgat gacgacccga gcggacgtaa cgtcagccgt
     3841 ggcatcgtgc tgctcgacca tgctgaacgc gatggtctgg acggatttat caccatcacc
     3901 ggtggcaaac tgatgaccta tcggctgatg gctgaatggg ctaccgacgc cgtgtgccgc
     3961 aaactgggca acacgcgccc ctgtacgacc gccgatctgg cacttcctgg ttcacaggaa
     4021 cccgctgaag ttaccttgcg taaagtcatc tccctgcctg ccccgctgcg cggttctgcg
     4081 gtttatcgtc atggcgatcg cacgcctgcc tggctgagcg aaggtcgtct gcaccgtagc
     4141 ctggtatgtg agtgcgaagc ggtaacagcg ggtgaagtgc agtacgcggt agaaaattta
     4201 aacgttaata gcctgctgga tttacgccgt cgtacccgtg tggggatggg tacctgccag
     4261 ggcgaactct gcgcctgccg cgctgccgga ctgctgcaac gttttaacgt cacgacgtcc
     4321 gcgcaatcta tcgagcaact ttccaccttc cttaacgaac gctggaaagg cgtgcaaccc
     4381 atcgcctggg gagatgcact gcgcgaaagc gaatttaccc gctgggttta tcagggactg
     4441 tgtggtctgg agaaggagca gaaagatgcg ctttgatact gtcattatgg gcggcggcct
     4501 cgccggatta ctctgtggcc tgcaactgca aaaacacggc ctgcgctgtg ccattgtcac
     4561 gcgtggtcaa agcgcgctgc atttctcatc cggttcgctg gatttgctga gccatctgcc
     4621 tgatggtcaa ccggtgacag acattcacag tggactggaa tctttgcgtc agcaggcacc
     4681 agcccatcct tacagtcttc tcgaaccaca acgcgtgctc gatctcgctt gccaggcgca
     4741 ggcattgatc gctgaaagcg gtgcgcaatt gcagggcagc gtggaactgg cccatcagcg
     4801 cgttaccccg ctcggcactt tgcgtgcaac ctggttaagt tcgccagaag ttcccgtctg
     4861 gccgctaccc gcgaagaaga tatgtgtagt ggggataagc gggctgatgg atttccaggc
     4921 gcatcttgcg gcggcttcgt tgcgcgaact ggatctttcg gttgaaaccg cagaaataga
     4981 gctgccggaa ctggatgtcc tgcgcaataa cgccaccgaa tttcgcgcgg tgaatatcgc
     5041 ccgtttcctt gataatgaag aaaactggcc gctgttactt gatgcgctta ttcctgtcgc
     5101 caatacctgc gaaatgatcc tgatgcccgc ctgcttcggc ctggccgatg acaaactgtg
     5161 gcgttggttg aatgaaaaac taccttgttc actgatgctt ttgccaacgc tgccgccttc
     5221 cgtgctgggc attcgtctgc aaaaccagtt acagcgccag tttgtgcgcc agggtggcgt
     5281 gtggatgccg ggcgatgaag tgaaaaaagt gacctgtaaa aatggcgtag tgaatgaaat
     5341 ctggacccgc aatcacgccg atattccgct acgtccacgt ttcgcggttc tcgccagcgg
     5401 cagtttcttt agtggcggac tggtagcgga acgtaacggc attcgcgagc cgattctcgg
     5461 ccttgatgtg ctacaaaccg ccacgcgggg tgaatggtat aagggcgatt tttttgcgcc
     5521 gcaaccgtgg cagcagttcg gtgtaaccac tgatgagacg ctacgcccgt cgcaggcagg
     5581 gcaaaccatt gagaacctgt ttgccatcgg ttcggtgctg ggcggatttg atcccatcgc
     5641 ccaaggatgc ggcggcggtg tttgtgccgt cagtgcttta catgccgctc aacagattgc
     5701 ccaacgcgca ggaggccaac aatgaacgac accagcttcg aaaactgcat taagtgcacc
     5761 gtctgcacca ccgcctgccc ggtgagccgg gtgaatcccg gttatccagg gccaaaacaa
     5821 gccgggccgg atggcgagcg tctgcgtttg aaagatggcg cactgtatga cgaggcgctg
     5881 aaatattgca tcaactgcaa acgttgtgaa gtcgcctgcc cgtccgatgt gaagattggc
     5941 gatattatcc agcgcgcgcg ggcaaaatat gacaccacgc gcccgtcgct gcgtaatttt
     6001 gtgttgagtc ataccgacct gatgggtagc gtttccacgc cgttcgcacc aatcgtcaac
     6061 accgctacct cgctgaaacc ggtgcggcag ctgcttgatg cggcgttaaa aatcgatcat
     6121 cgtcgcacgc taccgaaata ctccttcggc acgttccgtc gctggtatcg cagcatagcg
     6181 gctcagcaag cacaatataa agaccaggtc gctttctttc acggctgctt cgttaactac
     6241 aaccatccgc agttaggtaa agatttaatt aaagtgctca acgcaatggg tatcggtgta
     6301 caactgctca gcaaagaaaa atgctgcggc gtaccgctaa tcgccaacgg ctttaccgat
     6361 aaagcacgca aacaggcaat tacgaatgta gagtcgatcc gcgaagctgt gggagtaaaa
     6421 ggcattccgg tgattgccac ctcctcaacc tgtacatttg ccctgcgcga cgaatacccg
     6481 gaagtgctga atgtcgacaa caaaggcttg cgcgatcata tcgaactggc aacccgctgg
     6541 ctgtggcgca agctggacga aggcaaaacg ttaccgctga aaccgctgcc gctgaaagtg
     6601 gtttatcaca ctccgtgcca tatggaaaaa atgggctgga cgctctacac cctggagctg
     6661 ttgcgtaaaa tcccggggct tgagttaacg gtgctggatt cccagtgctg cggtattgcg
     6721 ggtacttacg gtttcaaaaa agagaactac cccacctcac aagccatcgg cgcaccactg
     6781 ttccgccaga tagaagaaag cggcgcagat ctggtggtca ccgactgcga aacctgtaaa
     6841 tggcagattg agatgtccac aagtcttcgc tgcgaacatc cgattacgct actggcccag
     6901 gcgctggctt aaactccttt ctgatgcccg gtaagcatgt ggttaccggg catttttgcg
     6961 tacacgattc cgtgcccaat gtatgcgttg caacgcagtg aaaattcctc tgaaaacgtc
     7021 tcgcaaaggc tgaaactggc agatgtcaaa ggcctgggat aaccgtaatg tcgcgtcatc
     7081 ataaatatca ggtgacggac aaccatgacc gaatcaacaa cctcctcccc gcatgatgcg
     7141 gtatttaaaa cctttatgtt cacacccgaa accgcacggg attttctcga aatacatttg
     7201 ccagaaccac tgcgcaagct ttgcaacctg caaaccttac gcctggaacc cactagtttt
     7261 attgaaaaaa gtttacgcgc ttactactcg gatgttttgt ggtccgtgga aaccagcgac
     7321 ggtgacggct atatctactg cgtgattgaa catcaaagct ctgcagaaaa gaatatggct
     7381 tttcggctaa tgcgctatgc cactgccgcc atgcagcgtc acctggataa aggctatgac
     7441 agagttccgc tggtggtgcc attgctgttt tatcatggcg aaacatcgcc ctacccgtac
     7501 tcacttaact ggctggatga gtttgacgat ccgcaacttg cccggcagtt gtacaccgaa
     7561 gcttttccgt tggtggatat caccatcgta cctgacgatg agatcatgca acatcggcgt
     7621 atagctctgc ttgaactgat tcaaaagcat attcgcgacc gcgatttaat cggtatggtc
     7681 gacaggatca ccacgctttt ggttaaaggc ttcactaatg acagccagct acaaacactg
     7741 tttaattatc tgctgcaatg cggcgatacc tcccgtttca cccgttttat tgaggagatt
     7801 tccgaacgtt caccactaca aaaggagaga ttgatgacta ttgctgagcg gctacggcag
     7861 gaaggacacc aaattggctg gcagaaaggt atgcatgaac aagccattaa aattgctttg
     7921 cgcatgctgg aacagggcat tgatcgtgac caggtgctcg cggccaccca gctaagcgaa
     7981 gccgatctgg cagcgaataa ccactaatta acacaggccc acagccgatc cccatgggcc
     8041 tttgatatca ataactacct tttatgcgtg gcccattttt gcctgattta aacatcgcca
     8101 gtcgttgatc cagggcatcg ctgtagagca tcgtgtcaac gccaacagcg acaaagttcg
     8161 ctccccacgc caggcattgc tgcgccatat caggagccac agccagaaaa ccagccgctt
     8221 tacccgcagc acggatccgc cgaatgctgg tttcaataat tcgctgcact tccgggtgcc
     8281 cggcgttatc cgggtagccc aacgacgcag aaagatccgc tggtccaata aacacgccat
     8341 caatcccttc gacgtcgagg atctcgtcca ggttatccag tgccgtttta ctttccacct
     8401 gcactaacag acaaagcgaa tcgttgactt gcgccatgta attctcaatg cgtccccagc
     8461 gcgcagcccg tgccacactg gccccaacac cacgctcacc gtagggagga tagcgcgtgg
     8521 cagacgccac ctgacgagcc tgatcggcag tatcgaccat cgggatcaat agagtttgcg
     8581 cgccaatatc cagaacttgt ttaatcagcg atttactgcc ttccaccgga cggatcacgg
     8641 gttggctggc atagggcgct accgcctgta gctggtgata aagatcctga atggtgtttg
     8701 gcgcgtgctc cccgtcaatc agcaaccagt cataaccgga agtagcggca atttctgcca
     8761 tataagaagt agtcgagctt aaccacagac caatttgcac ttcgcccttg cgtaaacgtt
     8821 ctttaaaggg attggttaat aatgcgttca tcgtgttcct taacctattt aatgatgtgc
     8881 cacgtcggtc tgcgcaacag tacgatttac acgcagagtg aaaataatca gcgaaccgat
     8941 gaccgccacc gctgccagtg tcagtaatcc tgccgcatcg ctggcaaata acgtttctgc
     9001 tttcacgcgc aggatcgggg caataaaccc gcccactgca ccaaacaggt ttacaaaacc
     9061 aatccccgca gccagcgccg taccggaaag cagttgtgtc ggcatcgtcc agaacaccgg
     9121 ctgcacggca ataaacccga tagctgcaac acacagcgct acgatcgcca ttactggaga
     9181 aagcagcccg gacagaccaa taccaatgcc cgccgccagt aatgtcagcg ctgcgacatt
     9241 acgccgttcg ccggttttat cggaatagcg cggaataagc caggtcccaa acaaggccgc
     9301 aacccacgga atggcggtga ccaccgacgc tgtaaagccc acttttgttc ccagcaatgc
     9361 cgcaacctgg gtcggcagga agaaaattaa tccgtaaacc gccacctgaa tggtcaggta
     9421 gataatcgct agttgccaga ctcggccatt acgcagcgca tcgctcagcc gagaagtcac
     9481 tttctgttgt tcttcacttg ccagttgatt gataagcaac gttttttctt gtttactcag
     9541 aaaacgtgcc tgctccggtg tgtcatcaag ccaaaagaat gtgaataccc cagcaccgac
     9601 tgccaacaat ccttcaatca caaacatcca gaaccagccg ggatgcccca taaatccatg
     9661 catctccaac agcgcgccgg aaagcggtga tcccagtgtt aacgccagcg gtgcgcccat
     9721 atagaacagc cccataatgc tggcgcgatt acgctgcgga aaccattgcg aggtgagata
     9781 aatcatacca gggaaaaatc cggcctccgc agcaccaagc agagtgcgaa ctatcagaaa
     9841 tttcgcttca gtatcggccc atgccatggc tgccgaaaga aatccccaca gcagtgttgt
     9901 cgtaccaatc caggttctgg cccccagttt gcgcatcaaa agattcgccg gaacacccag
     9961 aaacgcatat accacaaaga aaatgcctgc tcccagcgca taagcttcat tactcaaccc
    10021 ggtatcaatc tggtaggtct gtttggcaaa accgatattc gaacggtcga gaaacgccag
    10081 cacatacagc gccaacataa acggaattaa acgcacacgg tttttcttca ccacggcgtc
    10141 aagcaaagcg gtgctcataa aaagctcctt agaatatgtg ggcgttaccg ttgtcacagc
    10201 aacgcccgga gtgattagtg gctgtagggg cgtttcaaat tgcagtcacg attgagttcg
    10261 acgccaaaac cgggtttatc aagcactgat ttatgaatac gaccattcac cggaaccggc
    10321 tcattgagca gaatcgggtc aaactgcgga cgcatcgttg aacaatccgg gctggtcatc
    10381 aggaattcgc tgaatggcgt attggtgaag gtgatcaccg catggtgaga gtaaacagac
    10441 gaaccgtgcg gcaccaccag ttgccccctg gatttggcga ttgcggcaat ttccaccagc
    10501 gtggttaaac caccgcacca gccaacatcc ggctgcataa tgtcgatacc ggtttctgaa
    10561 agcgtacgaa aagattgcag tgtgccgtgg tgctcaccgc tggtgaccat cattccgact
    10621 ggcgcgttgc gtttcagttc gcgataacct tcatactgct gtggcggcag gcactcttcg
    10681 atccatttca ggttataggg cgcgcaagcg tgggccagtt tggtggcata gttcacgtcc
    10741 tgactcatcc agcagtcgag cattaaccag aaatcctcac cgcatttttc acgcatatcc
    10801 gcgaccataa cggcatcttt gcggatcccc gcatcgccat catgtggccc ccagtgcgtc
    10861 ggcattttgc caccgataaa gcccatctct tttgccagat ccggacgcgc gcctgtggcg
    10921 tagaactgaa tctcatcacg aactgcgccg cctaaaagtt tataaaccgg aagcccgacc
    10981 actttgccga acagatccca cagagccaga tcgacacagg aaatcgtgtt catcaccagg
    11041 ccaccagagc cggagtaata cagggtggca ttgagcattt gatcatggat cagtttgata
    11101 tcactgacac atttaccctc aatgaaacgg ttaagatgtt tttcgacaat aaaacagccc
    11161 atttcaccgg ctgtcgaaac agcgaatccg gtctggccgt tttctgcttc gacttcaaca
    11221 atcaacgtgc caagaacgtt aatgccaaat gactggcgtg actgctcgta atcgcggtat
    11281 ttactcatcg gggtggcaat atgatcgtca atccagtgat tcgccccctg gtcgtgataa
    11341 tcaccgcgcc agcacctttt tctgctgtcg caccgccagt aaaccaggcg cgaacctgtt
    11401 tgatttttgg tagggtcatg atgttctcca ttgttatgag gcctgtaagt caaagggact
    11461 tttccatccc aacagacgtg aaatatccct ggcgcaggca atggccttgc ccgccagata
    11521 atcacggtat tcttcattga tttgtaagcg ggtaccgacc accgagatcg cagcggtaag
    11581 ctcgttattg gcgttaaaca ccggcgcagc gacacaacgg acatcggggt aatcttcgcc
    11641 gttgtcatag ctccagccct gacggcgaat acgcgccagt tcttcgtgaa gttgctgtgg
    11701 atgagtaatc gttgtgggtg tcgcctgctc ccagaccagc ccttcgataa tactttgctg
    11761 taccgctgca ggttgccagg caagcaggca tttacctatt ccggagcgat aaagcgaaag
    11821 gctttttcct tcatgggaac gcacgctgat agtggctgat gactccactt ttaaaatgta
    11881 ataagcgctg ccgttatcaa taatccccag gtgacataac agcccggtgg tatccatcag
    11941 ttgcgtcaaa cgcggtcgag ccagttcccg gagatccatc ttgcttaacg catgaccaga
    12001 aagctccacc agcctggtcc acaaacaaaa attttcctgg tgatcaaggc taagaaaacg
    12061 ctgacgcctg agttcattaa gcagcaaata ggccgtgctt ttagggattc ccaatgtgtc
    12121 aatgatcgtc gcagcactac aggggccaat gcgggcaatc aaattgagaa tatcaatagc
    12181 ccgtgtcagg gcgggaactt tgcttgattc caacatactg gactccagtc ttagatactg
    12241 gaatcagtct cgttgctcag ggggagtaaa attgtgaagc gcatcaaatc ccgctcctcg
    12301 tccagcggac tggagaaatt acgcacaaat ttgtgaatca gtgcacggat tttttcttat
    12361 tgagtacaac aggttggaca agttttgccg gagggggaat gtgccggtgc agtaaaccgg
    12421 cacagagtgg cggaatcaga cagataaggt catggactca acaacctcaa tccagccatg
    12481 ctcactggcg atatcctggc cgtttaacca acggcgcagc atattcagtg ccatcattgc
    12541 gcacacttcc tgacggatag ccaggctgta gcgtgtagtg ctgaaacgca cacgcagagc
    12601 gaaagtgccg tctggcgtgg ctagcgcaaa gttgagatgc tcgttctcga aacccgaaac
    12661 agccagtgcc agcccggcaa aatggttggc ccgccgttct gtaatccagt gcgcagtttg
    12721 cgccagggtt tcttcctgtg aaggaaccac ttcacacgcc agcaatggag cacctgcgcg
    12781 agaaagttgc aaagccaata aaccaccggt gaattgctcg ctcaacgtca ggctgaactg
    12841 gcggttttgc aattcgcgac tgatctgcgc gggcagtcct tcggtgcctt cgaaaatcac
    12901 gctctgtccg gcaacacgtt taacgtccag ccacagtttt tccatcgcct gttgctcgct
    12961 tgccggtccg gtgagtttca gttcgatgat cggcattgag gagcgatagc ccattgttac
    13021 gcccggcggc agttgtagag tgtccaggct ttgtgccaga tcgctttccg aacgaccaaa
    13081 ggtagttaaa cgcagacaaa ccggcgactg tggtaaagaa aaacgctcgc gcaggcgcgg
    13141 caggatttcg tgctcgacca tcaccttaaa ttctgacggt acgccgggag taaagaacat
    13201 caggcaacga ttaagccgca cggcaaaacc acaggcggtg cctaccgggt tattgataaa
    13261 ttcagcactg gcaggcagct ccgcttgttt acggttgctc ggtgccatta ctcgtccacg
    13321 ttcgtggaaa tagcgttcca tctctttgag ccaggcttca tgcagcacca ggccttcacc
    13381 ttttgctgtc gcagcggcga gtgcacttaa atcatcactg gtcggtccca gcccgccgtt
    13441 aacgatcagc acatcggcgt gctgactacg ttcgcgaaga atggtgacta agtcatcaag
    13501 gttatccccc accgtattgc ggcgagataa tggcaacccc tgatgaaaga aaaaatcggc
    13561 cagccaggca gcgttagtgt caacgatttg cccgtgtaac acttcatccc cggtggataa
    13621 catttccact tttaacatgg ttatctcctg cttttgttat cgaatcacta taacgcaaag
    13681 ggagataagg cgtagtaaga aactgaatgg cgcgacagaa tgccgcgccg gaagggatca
    13741 gaaactggcg ttaaccccaa catacgggcc gtcagccacg gcgttgtcgc ggttaccgtc
    13801 tttacccgac agattcaggt agcgataacc cgcctcaata ctgactggat gcataatggt
    13861 gtaacgcgcg ccagcattcg cttcctcata acttttaata ccgctggaga gtgaatccgg
    13921 agagtagtaa tactcgccaa acaaacggaa gctgttgcca attttccact gcaaaccacc
    13981 tcctaccgct gcggcataac cttcatcgcc gtaattcggg ttggtgtaaa cgccttttcc
    14041 gccaacggtc gccattaacg gcccgagagg cagattcaac cccagcccca cgccagcgac
    14101 gtcaccgtcg tcgtcgttat gtgtccagtt accgcttaac gccaggcccg tcgattcagt
    14161 cccaaaaccg acaccaatat tggtgtattc tttacccgcc tgaccgctga tgctcattgc
    14221 attgaccgat gcagaaacca acagcattcc ggccagacct gctagcgcaa tttttttcat
    14281 tatccccttt cccacaagca attaatcaaa attcccaaag ctgcgggatt gtactgcctg
    14341 gcacagagga ttcaatgagc gaaaaaaggg atctagtcac ttattgtaac taaatacttc
    14401 ttaaataatt aaacgccgaa aaaataaaca cactttaccg atacacacaa aaataataat
    14461 aagtgcagtt atcatcgcaa aagagattac tgtcactttc acatatttct gagttaccgt
    14521 attctcatgg tttcttttta tttataagga atacccctgt gcgacaacgg actattgtat
    14581 gccctttgat tcaaaatgat ggtgcttatt tgctgtgcaa aatggccgac gatcgcggcg
    14641 ttttccccgg tcaatgggcg ctttcgggtg gcggcgtgga gtctggcgaa cgaattgaag
    14701 aggcactacg ccgcgaaatt cgcgaagaac tgggagaaca gctgcttttg acagaaatca
    14761 cgccgtggac cttcagcgat gatattcgca ccaagacgta tgcagatggt cgcaaggaag
    14821 agatttatat gatttacctg atttttgact gcgtttctgc caaccgagaa gtgaaaataa
    14881 acgaagagtt tcaggactac gcgtgggtaa aacctgaaga tctggtgcat tatgatttaa
    14941 atgttgccac ccgaaaaacg ttacgtttga aaggtcttct ttaacaatga tgacagccat
    15001 cactggctgt cagattgaaa atttagtggt taacgaattc cccatccaga taaactttgc
    15061 ctttttccac atgcatgact aaaccatcca gataatcagg tttaaatgtc gcatcacgct
    15121 tatttttagc aatatatgtc aggcaatgat tatgggtgaa aataacgata tttttatcag
    15181 gcgctttgct ttgtaagtcc ttaattgcac tataaatctc attaccgcac tgaagaagtc
    15241 gtttatctac cgtcagtttt ttacccgcgg aaaaccaggt cgccgactga atggtccgga
    15301 cggtattact ggaataaaga tcgaaatcag ggatgtcagc gctaaaagcg ttgcccagtt
    15361 cacgggcatc ctgggtacct ttaaccgtaa tacctgtttt atctgacaag cattgattgg
    15421 ttgaacgatc gcaacgttca gcatggcgaa acaaaacgac aaccgggtgc tgctgtgcca
    15481 gtctggccaa tgttttattg tcgatacgtg gcaaaccatt gctactccag gcggcatgag
    15541 tacccagtcc ggcaattgca gcgagcgcca gtaaaatgat gaaatatttt tttgacttca
    15601 atgatgagcg gcaaaaagct aacacgtaaa ctccacctat agacaagcgc aaccagacaa
    15661 ttaccgtgaa attgagcgac atttctggcg ataattagca gttggtgtaa tattaaaaat
    15721 cctacgatgt cggcaaaatg cctcaaaatt ttgccaaatg caaagcctaa ataagaaaaa
    15781 atataaaaat ttcaatattt acgtctaata ttagtttctt aaggttaagt taatattcta
    15841 tccttaaaat ttcgctccaa atggcaaaat atacacaaca ctctttatag caaatataag
    15901 tggacaggta ttcaatggcg gaaggaaaag caatgtcaga atttttgcct ttttcgcgac
    15961 cagcaatggg cgtggaggaa ctcgctgcag ttaaagaggt tctcgaatcc ggttggatca
    16021 caaccggtcc gaagaatcag gcgcttgagc aagccttttg ccagttgacg ggaaatcagc
    16081 atgccatcgc ggtcagttca gccaccgccg gaatgcatat cacgctaatg gcgttggaaa
    16141 ttggcaaggg cgatgaagtg attacgcctt ccctgacctg ggtttcaacc ctcaatatga
    16201 tttccttgtt gggtgcaacg ccggtaatgg tggatgtcga ccgcgatacg ctgatggtca
    16261 cgcctgaagc gatcgagtca gccattacgc cacgcactaa agccatcatt ccggtgcatt
    16321 atgccggtgc gccagcagat attgacgcca ttcgcgccat tggcgaacgt tacggcatcg
    16381 cagttatcga agatgctgcc catgccgtcg gtacgtatta caaagggcga catattggcg
    16441 caaaaggtac cgctattttt tcatttcatg ccattaaaaa tattacctgt gcggaaggtg
    16501 gcctgattgt aactgataat gaaaaccttg cccgccagct acggatgctg aaatttcacg
    16561 gtctgggtgt cgatgcctat gacagacaaa cctggggccg tgcaccgcag gctgaagtct
    16621 taacaccggg ctataagtac aatctgaccg atattaacgc cgcgattgcc ctgacacagt
    16681 tagcaaaatt agagcacctc aacacccatc ggcgcgaaat tgcccagcaa tatcagcaag
    16741 cactggcagc tctccccttt cagccattaa gccttcccgc ctggccgcac gttcacgcct
    16801 ggcatctgtt tattattcgt gtcgatgaac aacgttgtgg tatcagtcgc gatgcgttga
    16861 tggaagcgtt aaaagaaaga ggcattggta ccgggttaca tttccgcgcc gctcacacac
    16921 aaaaatatta tcgcgagcgt tttcccacgc tgtcgttacc gaataccgaa tggaatagcg
    16981 aacgcatctg ttctttgccg ctgttcccgg atatgactac cgccgatgcc gaccgtgtca
    17041 tcacagccct tcagcaactc gcaggacaat aagccatgtt tgaaatccac cctgttaaga
    17101 aagtctcggt ggttattccc gtttataacg agcaggaaag cttaccggaa ttaatcaggc
    17161 gcaccaccac agcctgtgaa tcgttgggga aagagtatga gatcctgctg attgatgacg
    17221 gcagtagcga taattccgcg catatgctgg tcgaagcctc acaagcggag aacagccata
    17281 ttgtgtctat tttgcttaac cgcaattacg ggcaacattc agcgattatg gcgggattca
    17341 gtcacgttac tggcgactta attattaccc ttgatgccga tctccagaat ccgccagaag
    17401 aaatcccccg cctggtggca aaagccgatg aaggttacga cgtggtaggg actgtacgcc
    17461 agaaccgcca ggacagctgg tttcgtaaaa cagcttcgaa gatgattaac aggcttattc
    17521 agcgcaccac cggcaaagcg atgggtgact acggttgtat gctgcgcgcc tatcgccgtc
    17581 atattgtcga tgcgatgttg cactgccatg aacgtagcac ctttatcccg attctggcga
    17641 atatcttcgc ccgccgtgcc attgaaattc cagtacatca tgccgagcgt gagtttggtg
    17701 aatccaaata cagttttatg cgcctgatta atttgatgta cgacctggtg acctgcctta
    17761 ccacaacgcc gctacgtatg ctgagtctgc tcggcagcat tattgcgatt ggaggtttta
    17821 gcattgcggt gctgctggtg attttacgcc tgaccttcgg accacaatgg gcggcagaag
    17881 gcgtctttat gctatttgcc gtgctgttta cttttattgg cgctcagttt atcggcatgg
    17941 gattgctcgg tgaatatatc ggcaggatct acaccgatgt ccgcgcccgc ccccgctatt
    18001 ttgttcagca agttatccgt ccatccagca aggaaaatga ataatgaaaa ccgtcgtttt
    18061 tgcctaccac gatatgggat gcctcggtat tgaagctctg ctggctgccg gttacgaaat
    18121 tagcgccatt tttacccata ccgataatcc cgctgaaaaa gccttttatg gttcggtggc
    18181 tcgtctggcg gcggaaagag gcattccagt ttatgcgccg gataacgtta atcatccgct
    18241 gtgggtggaa cgcattgccc aactctcacc agaggtgatt ttctcttttt attatcgcca
    18301 tcttatttgc gacgagattt tgcagctcgc tcccgcaggt gcatttaatc tgcacggttc
    18361 gctgttacca aaatatcgtg gtcgcgcgcc gctgaactgg gtgctggtga acggtgaaac
    18421 ggaaacgggc gttacattgc accgaatggt aaaacgtgcc gatgctgggg ccattgtggc
    18481 gcaactgcgc gttgccattg cgccagacga tatcgctatt acgctgcatc ataaattgtg
    18541 ccatgccgcg cgccagctac tggaacagac attacccgcc attaaacacg gtaatattct
    18601 ggaaattgcc cagcgcgaaa acgaagccac ctgttttggt cacagaacgc cagatgacag
    18661 tttccttgaa tggcataaac cggcatccgt actgcacaac atggtacgtg ccgttgccga
    18721 tccgtggccg ggtgccttca gctatgttgg caatcagaaa ttcaccgtct ggtcgtcgcg
    18781 tgttcatcct catgccagca aagcacagcc ggggagcgtg atttctgttg cgccactgct
    18841 gattgcctgt ggcgatggcg cgctggaaat cgtcaccgga caggcgggcg acggcattac
    18901 tatgcagggc tcgcaattag cgcagatgct gggcctggtg caaggttcac gcttgaatag
    18961 ccagcctgcc tgcaccgccc gacgccgtac ccgggtactc atcctcgggg tgaatggctt
    19021 tattggcaac catctgacag aacgcctgct gcgcgaagat cattatgaag tttacggtct
    19081 ggatattggc agcgatgcga taagccgttt tctgaatcat ccgcattttc actttgtcga
    19141 aggcgatatc agtattcatt ccgaatggat tgagtatcac gtcaaaaaat gtgatgtcgt
    19201 cttgccgctg gtggcgatag ccacgccgat tgaatatacc cgcaacccgc tgcgcgtatt
    19261 tgaactcgat tttgaagaga atctgcgcat tatccgctac tgcgttaagt accgtaagcg
    19321 aatcatcttc ccgtcaactt cagaagttta tgggatgtgt agcgataaat acttcgatga
    19381 ggaccattct aatttaatcg tcggcccggt gaataaacca cgctggattt attcggtgtc
    19441 aaaacaatta cttgatcggg tgatctgggc ctatggcgaa aaagagggtt tacagttcac
    19501 cctcttccgc ccgtttaact ggatgggacc acgactggat aaccttaatg cagcgcgaat
    19561 tggcagctcc cgcgctatta cgcaactcat tctcaatctg gtagaaggtt caccaattaa
    19621 gctaattgat ggcggaaaac aaaaacgctg ctttactgat atccgggacg gtatcgaggc
    19681 gttgtaccgc attatcgaaa atgcgggaaa tcgctgcgac ggcgaaatta tcaacattgg
    19741 caatcctgag aacgaagcga gcattgagga actgggcgag atgctgctgg cgagcttcga
    19801 aaaacatccg ctgcgccatc acttcccgcc gtttgcgggc tttcgtgttg tcgaaagtag
    19861 cagctactac ggcaaaggat atcaggacgt agagcatcgt aaaccgagca tccgcaatgc
    19921 ccgccgctgc ctggactggg aaccgaaaat tgatatgcag gaaaccatcg acgaaacgct
    19981 ggatttcttc ctgcgcaccg ttgatcttac ggataaacca tcatgaccaa agtcggctta
    20041 cgcattgatg tcgatacctt tcgtggcacc cgtgaaggcg tgccacgtct gctggaaatc
    20101 ttgagtaagc ataatattca ggccagcatt tttttcagcg tcggcccgga caatatgggc
    20161 cgccatctct ggcgactggt gaagccacag tttttgtgga agatgctgcg ctcaaacgcg
    20221 gcatcgcttt atggctggga tattttactg gcaggtacgg cctggccagg taaagagatt
    20281 ggtcatgcca atgccgatat cattcgtgaa gcggctaaac atcacgaagt cggcctgcac
    20341 gcctgggatc accatgcctg gcaagcccgt agcggtaact gggatcggca aacaatgatc
    20401 gacgatattg cacgcggtct tcgcactctg gaagagatta tcggtcaacc ggtaacctgt
    20461 tctgccgctg cgggctggcg tgccgaccag aaggtgatcg aagcaaaaga agcgttccat
    20521 ttgcgctaca acagcgattg tcgtggggcc atgccgttcc gtccactgct cgaatcagga
    20581 aaccctggca ctgcgcaaat tccggtgacc ttacccacct gggatgaagt gattggtcgg
    20641 gatgtgaaag cagaagattt taacggttgg ttactcaacc gcatcctgcg agataaaggc
    20701 acgccggttt ataccattca tgcagaagtt gaaggctgcg cttatcagca taattttgtg
    20761 gatctcctca aacgcgcagc tcaggaaggc gtgacatttt gccctttaag cgaactgtta
    20821 tcagagacgt tgccgctcgg acaagttgtt cgcggaaata ttgccggacg tgaaggctgg
    20881 ctgggttgcc aacaaattgc gggtagtcgc tgatgaaatc ggtacgttac cttatcggcc
    20941 tcttcgcgtt tattgcctgc tattacctgt taccgatcag cacgcgtctg ctctggcaac
    21001 ccgatgaaac gcgttatgcg gaaatcagtc gagaaatgct ggcatccggc gactggattg
    21061 tgccccatct gttagggcta cgttatttcg aaaaacccat tgccggatac tggattaaca
    21121 gcattgggca atggctattt ggcgcgaata actttggtgt gcgggcaggc gttatctttg
    21181 cgaccctgtt aactgccgcg ctggtgacct ggtttactct gcgcttatgg cgcgataaac
    21241 gtctggctct actcgccaca gtaatttatc tctcattgtt tattgtctat gccatcggca
    21301 cttatgccgt gctcgatccg tttattgcat tctggctggt ggcgggaatg tgcagcttct
    21361 ggctggcaat gcaggcacag acgtggaaag gcaaaagcgc aggattttta ctgctgggaa
    21421 tcacctgcgg catgggggtg atgaccaaag gttttctcgc ccttgccgtg ccggtattaa
    21481 gcgtgctgcc atgggtagcg acgcaaaaac gctggaaaga tctctttatt tacggctggc
    21541 tggcggttat cagttgcgta ctgacggttc tcccctgggg actggcgata gcgcagcggg
    21601 agcctgactt ctggcactat tttttctggg ttgagcatat tcaacgcttt gcactggatg
    21661 atgcccaaca tagagctccg ttctggtact acgtgccggt catcattgcc ggtagcctgc
    21721 cgtggctggg attactcccc ggtgcactgt acacaggctg gaaaaaccgc aagcattccg
    21781 caaccgtcta tttgttgagc tggacgataa tgccgctgct gtttttctcc gtcgctaaag
    21841 gtaaattgcc cacctatatt ctttcctgct ttgcatctct ggcaatgctg atggcgcatt
    21901 acgctttgct ggcagcaaaa aataatcctc tggcgctgcg gattaatggc tggattaaca
    21961 tcgcttttgg cttcactggc attattgcca catttgtggt ctccccgtgg ggaccaatga
    22021 acacgccggt gtggcaaacc ttcgagagct ataaagtctt ttgtgcctgg tcgatttttt
    22081 cgctatgggc atttttcggc tggtacacct taacaaacgt cgaaaagacc tggtcttttg
    22141 ccgcgctttg cccgctgggg ctggcgttgc tggtaggatt ttcaattcct gacagagtca
    22201 tggaaggaaa acatccgcaa ttttttgtcg agatgacaca agaatcactt cagccaagcc
    22261 gctatattct tactgatagc gtcggtgttg ccgcaggtct ggcatggagc ctgcaacgcg
    22321 atgacatcat catgtatcgc cagacaggtg agttgaaata cggccttaat tatccggatg
    22381 cgaaagggag atttgtcagc ggtgatgagt tcgcaaactg gcttaatcaa catcgtcagg
    22441 aggggattat tacactcgtg ctttcggttg accgcgatga agatatcaac agtctcgcca
    22501 ttccgcccgc agatgccatc gatcgtcagg agcgtctggt gctgattcag tatcgtccca
    22561 aatgatctgg ctaacattag tctttgccag cttgcttagc gttgccgggc agttgtgtca
    22621 gaaacaggca acctgctttg tggcgataaa caaacggcgc aaacatatcg tgctgtggct
    22681 gggactggcg ctggcttgtc ttggtcttgc catggtgctc tggctgctgg tcttgcagaa
    22741 cgtaccggta ggcattgcct acccgatgtt aagtctgaat tttgtctggg tgacgctggc
    22801 tgcagtaaaa ctgtggcacg aaccggtatc gccgcgtcac tggtgtgggg tggcgttcat
    22861 tattggcggc attgtgatcc tcgggagtac ggtgtaatgg gcctgatgtg gggattattc
    22921 agcgtcatta ttgcctcagt tgcgcaatta agcctgggtt ttgcggcgag tcatctgccg
    22981 ccgatgacgc acttatggga ttttatcgcg gctctgttag cttttggcct ggatgcccgg
    23041 atactgttac tcgggctgct gggttatctg ctttccgttt tttgttggta taaaacgcta
    23101 cataaacttg cgctaagcaa agcctatgcc ttgctcagta tgagttatgt gctggtatgg
    23161 attgcttcaa tggtacttcc cggctgggaa gggacttttt cgctgaaagc actactggga
    23221 gtagcctgta ttatgagcgg gttgatgctg attttcctgc ccacgacaaa acaacgttac
    23281 tgagttttcc ctgccacttt acactgccgt tcccactcat cggggctata actgcttgcc
    23341 gagaggactt tcaccgtatg cagcttttca cgattaatac aataaagcgc attctgcaaa
    23401 ggcgagagta aatcccccac tttcacggcg aagtcactct tcacttcggc gatcattttt
    23461 atcgcgccgc cagcatcgca gagcataagt acatgtctgt tatcttgttt attgcagcac
    23521 gattttttga ccagccattc cattgcatta tcctgtttgc taagagtttt cacatcaatt
    23581 tacagattaa cagataaacc tattatcaac ggcaatggaa tggcaaaatt gcgaagtgaa
    23641 actaatgatt tcgtaatcct ggcgttgcac ccactctttt aacgcctgac gtgaaatttt
    23701 aataccgccg tttttcagct ccggcggtaa agttagccag cgcaccggtt gttgaaaacg
    23761 agccagctta tctttcaccc attcgccaag atcaacggtt tgctggtcat actccaccac
    23821 cgctaccggt cgatgaccaa actccttgtc ggcaacgggg acgataaaca cctgcaaaac
    23881 cgcaggatgt gcagcaatta cgcgctcgac ttcttccggc tgaataccct ctccgccact
    23941 gaagaacaga ttgtctaaac gtccgacaat ggtcagcttg ccattatgca tctcaccgcg
    24001 atcgcgcgta gcgtaccagc cttcgtcatt aaccagtgaa accagttgcc cgttacgcca
    24061 gtaaccttct gccatactgg cagcccgcag ccacacttca ttattaacga ttttcacttc
    24121 ccgacccggc agcggcgaac caacgtctgc caggccgtcg gcttctttcg cacacaccgt
    24181 ggaggcaaac tcggtcagac catagccgca aaagcaacga atcccctgct cgcgcgcctg
    24241 ttccgtcaac tcgaccggga tagccgcgcc gccaagtaac accgctttca gggaaacgga
    24301 actgcggtta accagcaaac gccagagttg tgttggcacc agtgaagcgt gagtacagcc
    24361 tgccagcatt tgctccaatg gctgtttatc acgcaccgtc atccgcgcac cagcgtataa
    24421 ccagcgccac ataattccct gaccggagac gtgaaacagc ggtaaagaga gcaaccagtc
    24481 atcgtgatcg ccaaacggaa tcagcgataa cacaccttgc gcactggcaa gatgggcctg
    24541 ataagtatgg acagcggctt tcggcaaacc ggtagaaccc gaggtcaacg tcattgagca
    24601 cagacgcgtc ggctgccacg tagcggcatg tgcgccttca acccgctgaa tgtgcagcga
    24661 cgttaatgcc ggaaacgtgt tttccccatc cggcaccagc gcaaattgca gcgtcagatt
    24721 gggcagcaat tcttcaagca acggttgcgg cagctgaggg ttcacgggca acacccgcgc
    24781 cccgcattgc agtaacgcca gccaggcgag cagcgtttgc ggcgtattcc acgcccgcaa
    24841 catcacgccg ctgccctcaa ccacaccctg cgccgcaaag cccgtagcta actcatcgac
    24901 gcgagcacaa agctcgcgcc agttgagttg ctcgtcatta agacgtaagg cgatggcttc
    24961 tccccgcact tgccgccagt gacgccacgg ccagtcagag aagatcataa caactgctcc
    25021 agtacatcag cgtccactaa cggcagtggg ctccccggcc agcgacgaac ctgctgcgcc
    25081 tgcatcagat ccagcgtgtc cagtcccgga atggtgtctg gcgttaacca ggcagcaatc
    25141 cgcgccagtt gcgttagacc taagctcgac tcaatggaag aactgatcac cgccgtcagc
    25201 cccagcgcat gcgccgcctg tacctgctcg cgtacttttt ccagactgcc cgtgagcgtg
    25261 ggtttgataa ctaccgcgcg cacgccctct tcagccacaa aggcaaaatc cggctcgcgc
    25321 aggctttcat cccaggcaat ggcaatgccg gtttcacggg caaacgctcg cgaatcatcg
    25381 cgggttttgc acggctcttc gagaaacgcg atgcggtggc gataatccgg gttaacatat
    25441 ttggcaaact gctgaccttt cagcggtgtc caggcgcgat ttgcgtcaag acgcaaatgc
    25501 agatccggaa ttgcctccag caacagatta accaccatgc cgtcgcgcac ggcttcgtac
    25561 aatcccactt tgaccttcgc cactttctcg cctggcatat cagcaagttt gaggatcaga
    25621 tcgtcaggat cgccattaca cagtggtgcc gcacggtaat tggccgcctg cgggagcgta
    25681 tctgccagct ccgccaatgc acagcttacg ccaaaggcaa cggaaggcat ctgcggtagc
    25741 tcgccatcgc ctgccagcca gttatttacc caagcaagca gcacaatttg cgcctcttcc
    25801 caggtttcct gactgaagcc cggcagtggg gagatctccc cccacccttc gcgctcgcct
    25861 tcacgcaggc aaacatacag cccgtcgcgg gtttttaacc gcctgtcgcg cagaaccatc
    25921 cccgcgtcca tggggatctg ccagcggtat acctgcgcgc tacgcattac ggattccgtt
    25981 taaatttgct gaaatcaggc tgacgtttct ggttgaaagc gttgcgacct tcctgacctt
    26041 cttccgtcat gtagaacagc atggtggcgt tgcccgccag ctcctgcaga cccgcctgac
    26101 cgtcacagtc ggcgttcagc gcagctttca ggcagcgcag cgccatcggg ctgttttgca
    26161 gcatttcgcg gcaccaacgg acggtttctt tttccagatc cgccagcggt accacggtgt
    26221 tgacaaggcc catatccagc gcctgttttg catcgtactg acggcacagg aaccagattt
    26281 cacgcgcttt tttctgtccg acgatgcgag ccatgtagga agcgccccag ccgccgtcga
    26341 aggaaccgac tttcgggcca gtctgaccga agatggcatt atctgccgcg atagtcaggt
    26401 cgcacatcat gtgcagaacg tgaccaccgc cgatggagta gccagccacc atcgcgacaa
    26461 ccggtttcgg acaggtacgg atctgacgct ggaagtccag cacgttcagg tgatgtacgc
    26521 cggaatcatc tttatagccg ccgtaatcac cacgcacttt ctggtcacca ccggagcaga
    26581 acgctttatc gcctgcgcca gtcagaatga tcacgccgac gttgtcgtca taacgcgcat
    26641 ctgccagtgc ctggatcatc tcttttaccg tcagaggacg gaaggcattg cgcacctgcg
    26701 gacgattaat ggtgattttt gcgataccgt cggtggattt ttcataacga atgtcctcaa
    26761 aaccttcgga gcagtcgtgc cattcaaccg gtgcgtaaag cattgcttca tcaggataaa
    26821 tcatattgtg tcctttagtc agaaacgcaa gatctgcgcc agacttgcga taacgccagc
    26881 gggattttcc cgatgcgcgt tatgtccggc gcgaggaatg acatggcagt cggcagccag
    26941 ttccgccgcc aaggcgcgga atttgctgtc acgttcacca cataaataat aaaacgcaaa
    27001 tgtgcgggcg ctaaggttag cacgtaaatc aggctggacg gcgagagaag tcgcctccag
    27061 catggcggca agggttgcgc cattattgtt actgcgcagc gccaccagct cccggcgttg
    27121 atcgtcattg agcgaggcaa aaacaggctg ttgataccag tcggcaaata ccgccgttaa
    27181 cggttctgtg cgaaaacgct gcgcccattg gcgatcggaa cgctgacgtt ccgcgcgttg
    27241 ttcagcattt tgcaacccag gatgcccgcc ttcgacaaca accccacaaa gccccggcat
    27301 cccctggcaa gccgccatca tcgccacccg tcccccaagc gagtacccca ccaaccagaa
    27361 gttaaggatg ttgtaactaa ccagggtttt acgcagtaag tcggtgacat catcaaatcc
    27421 atcgacgcta atcgccgccg aaccaccgtg acctgggaga taaacataca accgtgagta
    27481 gtcggcaaac gcctcgccca cttcttgcca ttcgtggcaa tcgccggaaa aaccgtgcaa
    27541 aaacaccagc cagggtaaac ctggtttgcc gtgttttgcc tgcgcgtgca ggatcataaa
    27601 tggcttacct gcgccagaag ttgctggagc gtttgcgcac catcggtgtc gttaaccacc
    27661 atttcaatta ccgtggtggt tggcgtgcgc caggcatcgg caagtgccgt ttcaagttcc
    27721 tgccagtttt gcggacgatg atatttcagc tcgaacatcg cggcggcgtg ctcaaaatgg
    27781 acgttttgcg gcatcagata gaaacgctcg cgctcgcttt tcggcgttgg cagcagcgag
    27841 aaaatttgcc cgccgttgtt gttcaccaca attaatatca gcggcgcgga aacctgacgc
    27901 aataacgcca gcgcgttgag atcgtaaagt gcggagagat cgcccacaat cgccagcgtc
    27961 ggtttgccgc ttgcccgctg aacgccggcg gcagtcgaga gcagcccgtc gataccgctg
    28021 gccccacggt tgctgtacac cgggtaacct gccggaagtt gcgaaagcgc atcaatcaga
    28081 cgtaccacca ggctgttacc gacaaacaat tgcccctgtt caggcagata gtcgctgatg
    28141 cgatgcgcca gttgcgcttc gccaaacgca tcgcgacggg caataaccgc ctgcattgcc
    28201 tgttccgcca ggcgcgggat ttcaacgcac cagggctggc gtttttctgc cggatgcagc
    28261 tccagccagt cggcaatatt ggcaattaag cggcgtccac gatggtgcgc cggatcaagc
    28321 cgcccttcga tgtcatccac aatccagtac tcttcgggtt cacagcttgc ctgccattgc
    28381 aggagccgtt tgcccgtcag gctgcttccc agttgcacca caatttgcgc ctgttgcagt
    28441 tcgctggtcg ctttggcatt gcctaaccag agatcggcac acggcagcgg ctgtccggtt
    28501 tgcgacagca cgtcgccaat cagcggccag ccaagagttt gcgcccacag tgcaactttt
    28561 ttgccctctt ccgcactcat gcgcccggca accaccacgc cgcgtttttg tcgccagaag
    28621 aaccagtcgc gctgtttttc actttccaga cgaggcgctt cacgcagcca cggtttgtcg
    28681 tcctgccacc agtcgcccag ccgctgttgc cagctaagcc cggtgtcgtc catttcgcca
    28741 tacagcggtt cggcaaacgg gcagttgata tggactccac cagaatgaag cgtaccgaga
    28801 gcatggtcga tggtagaaac cagccaacgt gcggggatat cctgggtcgg gcgcggcaat
    28861 gaaatactgt gcgtggggtg agaggcgaac attcccggct gacgaatcgc ctgattcgcg
    28921 ccgcagtcaa tcagctccgg cgggcgatcg gcggttaaca ggatcagttt ttctccggtt
    28981 aacccagctt caatcagtgc cggatagaga tttgctaccg ccgtaccgga ggtcacaatc
    29041 accgccaccg gctgcttgct gactttcgcc agccccagcg ccagatgccc caacccacgc
    29101 tcatcgaaat gggtgtggtg aatgaatgcg gaattctccg ccgccgctaa cgttaacggt
    29161 gtagaacgcg agccaggggc gatacagatg tgtctgacgc cgtgacgcgt taatgcttcc
    29221 agaatgaccg ccgcccagcg tcggttaaat gcgcttactg acatgagttt gtccggtatc
    29281 aatattgcgg ctaagtataa ggagctacaa aaatagatta ttgatatgaa tcggtaatga
    29341 tgcgactcat tactattcca tctgtaataa agtacgcagc cctgccgctt tgttgtcgat
    29401 ttcctgccac tcttgctcgg ggtcggaacc acggacaatg cccgcgccag catataatcg
    29461 cacgacattg ccgctaattt ttgctgagcg cagggaaacg cagaattcgc tttgctgtaa
    29521 tgagagatag cccgcagaac cggcgtacca ttctcgggtg aacggttcgt gacgggcgat
    29581 aaactgtcgc gccagatcgc gcggtaagcc agcaactgct gccgttggct gcaactgatg
    29641 taaacagatc acattatccg ctttgttgag tgaagtccag atacagcggc gaagatgctg
    29701 cactttacgc agacgcagta cctgcggcgg taaaacatcc agcgtctggg tatcggcctg
    29761 taaccgttgg cagatatctt ccaccaccag catattctcg cgctggtttt tatcatccgc
    29821 catcagccac tcacctaact gctgcgcctg cttatcatca ggatgatttg ctaccgttcc
    29881 cgccagcgct tcagtacgca gcgctttgtc acgccgccgc cataaccgtt ccggtgaaga
    29941 gccaagaaaa gcattttcgc catcaaaggc catgtaaaaa tggtagcaat tcagattcag
    30001 tcgacgactg gcagccatca tcgccgccgc gttgaccgga cttgcgaaat gcaggtcagt
    30061 tgcccgagcg agcaccactt tgtcgagctc accttcggcg atggttttcg ttgccagttc
    30121 gattaattgc gtccagcccg ttttgtccgg ccagtgttgt tctcgcgtgg tggttaaatg
    30181 taacccaggc aagggcttga tactcaccag tgtggcgata aattctcttg ccttaatcgc
    30241 atcgtgctga agggagcttt cgctgaatag cgtcagccgc agcgtggctt taccgccaca
    30301 gcgtcgccat tccaggcgtg gtaaaagtaa attgccctgc gacgggtcaa acgcattcag
    30361 cccccaaatg cgtaagtcgg cgtgttccgg gtgctggcga agaaaacgtt gtgcctggtc
    30421 caacgacgta aaacgggtaa tcgcgcccag gacggcagct tcttcatcac cattacgttg
    30481 ttgccagtag aattgcgggt atgtttgctg actggccagc cagctcaagg catcaaaagc
    30541 gtctttgaga gggaaaggaa tatcgataac ccgaatgccg ggtgtcgccg gaatctcttg
    30601 cgacaaatgg cgcagtagat tttccagcgc cgtagtaagt gattgcacgc ggacctctcc
    30661 ctgttaaaag cctcacatta tacggggtac tacaaaaaaa atgcagtacc ccggtgtagg
    30721 gaggttttaa cggcgtgcca gcaacagtcc tagtaccagc ccaacggccg cgcccacacc
    30781 aattccttgc cagggttttt cgtggacgta gtcatcagca cgataaactg cctgcttcgc
    30841 ccgatagtaa taactgtctg aagcctggct aacccgtttt ttcacatcat ccagcgcttt
    30901 ttctgcacgc gctttcagct caacatattt ctgatcggcg ggatcgccag aggagcggag
    30961 cacctcttcc agtgtttcac taagcagcgt caggtcgtca tcgatacgtg tatcaccaaa
    31021 ctgattagac attctcgttc tccatttgcg taaaacctgt cactaactat agacaagggt
    31081 tgttgattac gcctgaatta cctcgcgtgc catcccaatg tgtgggatac catcttcttc
    31141 atagacctcc gtcacaggga taaaaccgaa actctggtag aagttttgca gatgcgcctg
    31201 cgccccgagg taaacaggct tatcaggcca gtgatgcgtg caggtgtcga gggttttact
    31261 catcagttgc tgccccactt tttcgccacg caacgcttca ctgacaatca cccgaccaat
    31321 aacgaccggc tcaagatcat catcactttt cagaatcctc gcatatgcca ccagttcatc
    31381 atttttccaa ccgagaatat ggcgattatc ccccgtcaaa tcatcaccat cgatatcctg
    31441 ataagggcaa ttctgctcga caacaaacac cgcgcaacgc agttgtaata aggcataaag
    31501 ctgggaaacc gataattcag agtgatgcag atcttaccat tcaatcataa tgctctcctt
    31561 ttagatgtat gccttatact agccggaata ttttttgaaa cgtgatgaac tcatggaatt
    31621 aattttttta ggtacttcag ccggtgtgcc aacccgcacg cgcaatgtca cggcaatatt
    31681 gcttaacctg caacatccga cccagagcgg actttggttg tttgactgcg gtgaaggcac
    31741 ccagcatcag ctactgcata ccgcctttaa ccctgggaaa ctggacaaga tttttatcag
    31801 tcaccttcat ggcgatcatc tctttggttt acccggcttg ctgtgcagtc gttctatgtc
    31861 aggcattatc caacccttaa cgatttatgg tccgcaaggt atccgtgaat ttgtggaaac
    31921 cgcgctgcgg attagcggct catggaccga ttatccgctg gaaattgtcg aaattggcgc
    31981 tggcgaaatt ctcgatgatg gcctgcgcaa agtgaccgct tatccgatgg aacacccact
    32041 ggaatgttat ggctatcgta ttgaagaaca tgataaaccg ggtgcattaa atgcccaggc
    32101 attaaaagct gctggcgtgc cgcctggccc actgtttcag gaattaaaag cgggcaaaac
    32161 aatcacgctg gaagatggaa ggcagattaa cggcgcagat tacttag
//
LOCUS       DAJVRF010000035        30217 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJVRF010000035 DAJVRF010000000
VERSION     DAJVRF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30217
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.035"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..191)
                     /locus_tag="OQN13_001466"
     CDS             complement(<1..191)
                     /locus_tag="OQN13_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCS1614854.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERS"
     gene            complement(369..1280)
                     /gene="gsiD"
                     /locus_tag="OQN13_001467"
     CDS             complement(369..1280)
                     /gene="gsiD"
                     /locus_tag="OQN13_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCS1614855.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1283..2203)
                     /gene="gsiC"
                     /locus_tag="OQN13_001468"
     CDS             complement(1283..2203)
                     /gene="gsiC"
                     /locus_tag="OQN13_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCS1614856.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2221..3759)
                     /gene="gsiB"
                     /locus_tag="OQN13_001469"
     CDS             complement(2221..3759)
                     /gene="gsiB"
                     /locus_tag="OQN13_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCS1614857.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3779..5650)
                     /gene="gsiA"
                     /locus_tag="OQN13_001470"
     CDS             complement(3779..5650)
                     /gene="gsiA"
                     /locus_tag="OQN13_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCS1614858.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5637..6602)
                     /gene="iaaA"
                     /locus_tag="OQN13_001471"
     CDS             complement(5637..6602)
                     /gene="iaaA"
                     /locus_tag="OQN13_001471"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCS1614859.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6806..8041
                     /gene="moeA"
                     /locus_tag="OQN13_001472"
     CDS             6806..8041
                     /gene="moeA"
                     /locus_tag="OQN13_001472"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCS1614860.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8041..8790
                     /gene="moeB"
                     /locus_tag="OQN13_001473"
     CDS             8041..8790
                     /gene="moeB"
                     /locus_tag="OQN13_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCS1614861.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8969..9631)
                     /gene="fsa"
                     /locus_tag="OQN13_001474"
     CDS             complement(8969..9631)
                     /gene="fsa"
                     /locus_tag="OQN13_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCS1614862.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            9762..10661
                     /locus_tag="OQN13_001475"
     CDS             9762..10661
                     /locus_tag="OQN13_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCS1614863.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10667..13099
                     /locus_tag="OQN13_001476"
     CDS             10667..13099
                     /locus_tag="OQN13_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCS1614864.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13245..14060
                     /gene="ybiV"
                     /locus_tag="OQN13_001477"
     CDS             13245..14060
                     /gene="ybiV"
                     /locus_tag="OQN13_001477"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCS1614865.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14212..15477
                     /locus_tag="OQN13_001478"
     CDS             14212..15477
                     /locus_tag="OQN13_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCS1614866.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15444..16604)
                     /locus_tag="OQN13_001479"
     CDS             complement(15444..16604)
                     /locus_tag="OQN13_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1614867.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            16842..18143
                     /locus_tag="OQN13_001480"
     CDS             16842..18143
                     /locus_tag="OQN13_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1614868.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWGHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18158..20521
                     /locus_tag="OQN13_001481"
     CDS             18158..20521
                     /locus_tag="OQN13_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HCS1614869.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLEFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(20701..22296)
                     /locus_tag="OQN13_001482"
     CDS             complement(20701..22296)
                     /locus_tag="OQN13_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCS1614870.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPEILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22515..23435
                     /gene="ldtB"
                     /locus_tag="OQN13_001483"
     CDS             22515..23435
                     /gene="ldtB"
                     /locus_tag="OQN13_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1614871.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23494..24612)
                     /locus_tag="OQN13_001484"
     CDS             complement(23494..24612)
                     /locus_tag="OQN13_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCS1614872.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24609..25076)
                     /gene="mntR"
                     /locus_tag="OQN13_001485"
     CDS             complement(24609..25076)
                     /gene="mntR"
                     /locus_tag="OQN13_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCS1614873.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25262..25390
                     /gene="mntS"
                     /locus_tag="OQN13_001486"
     CDS             25262..25390
                     /gene="mntS"
                     /locus_tag="OQN13_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCS1614874.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            25662..27245
                     /gene="opgE"
                     /locus_tag="OQN13_001487"
     CDS             25662..27245
                     /gene="opgE"
                     /locus_tag="OQN13_001487"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCS1614875.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27294..27809)
                     /gene="ompX"
                     /locus_tag="OQN13_001488"
     CDS             complement(27294..27809)
                     /gene="ompX"
                     /locus_tag="OQN13_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCS1614876.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(27862..27927)
                     /locus_tag="OQN13_001489"
     CDS             complement(27862..27927)
                     /locus_tag="OQN13_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1614877.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28162..29049
                     /gene="rhtA"
                     /locus_tag="OQN13_001490"
     CDS             28162..29049
                     /gene="rhtA"
                     /locus_tag="OQN13_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCS1614878.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            29348..29851
                     /gene="dps"
                     /locus_tag="OQN13_001491"
                     /gene_synonym="pexB"
     CDS             29348..29851
                     /gene="dps"
                     /locus_tag="OQN13_001491"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCS1614879.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
BASE COUNT         7356 a         8245 c         7547 g         7069 t
ORIGIN      
        1 gaacgttcaa tatctttaaa gaaatagcca acgtttttct gaataagttc aaagcggcta
       61 tcggcacgtt ttaccgcgcg ttccgatacc agttgatagt taatgatgaa aaaaagacca
      121 ataaatgaca gtgctgccaa taaaaataaa attatcagcc ttatctttat cttttcgtat
      181 aaactcagca tgttaataca tttaacaaaa taactatctg aacgctgtgc cgctgcaaaa
      241 atagtccctt gctgcggctt tttaaccaaa ttacccgatt ctacaaaatc agcagaaagt
      301 tacaacactt gcttagggca ataatttcct gtgaggtgat taccctttca agcaatattc
      361 aaacgtaact atcctttaat tttcggatcc agcgcatcgc gtaaaccatc gcccaacaaa
      421 ttgaacgcca gtacggtcag aaaaatagcc agggccggaa aaacagcgac atgcggcgcg
      481 ataaccatat ccgctcgtgc ctcattgagc attgctcccc actctggtgt cggcggctgc
      541 gcaccgaggc cgagaaatga caggctggcg gcagagataa tcgaggtgcc aatgcgcatg
      601 gtgaaaaaca ccacgataga cgagacggtc ccaggcaaga tatgacgcaa taaaatggtc
      661 atatcgctgg caccaatact gcgtgctgac tcaataaagg tttgctgttt caacaccagc
      721 gtgttgccgc gcaccagtcg ggcaaacgcg gggatggaaa aaatggcgac tgcgataatc
      781 acgttagcaa tgccgcttcc caacaccgca acaacagcga tcgccagtaa aatacccggg
      841 aaggcaaaca gcacatcgca aatgcgcatg atcagccgat cccaccagcc ttcataatat
      901 ccagccaaca agcccagcaa cgtcccgatc gccgcaccga taaacacggc aaacacgccc
      961 gccgccagcg agatttgcgc accaaccagg acacggctga aaatgtcgcg ccccagtgaa
     1021 tcgacgccaa accagtgctg caaagaaggt ccgttattca gattgtcata atcaaaataa
     1081 ttttcggcgt catagggagc gatccagcgt gcaaaaatgg ccaccacaat caataaaata
     1141 acgaataatg cggcggtcat cgccatatgc tgacggcgaa atcgtcgcca gaattcatgc
     1201 cacggtgtac gcacctggtc aggtttgacc agtggcatgg cgtttaacac cgcctgacgt
     1261 cgccagttaa atagtcgcat ccttacttgt acctgatagc cgggttaatg gcggcgtaaa
     1321 gcacatccac cactaagttg ataagaataa attccagcga gaaaagcaga atttccgcct
     1381 gaatcaccgg gtaatcgcgc atttctacgg agtcaacgag taagcgtcca agtccgggcc
     1441 agttgaaaac tttctccacc acgatagaac caccgagcaa aaagccaaac tgtaagccca
     1501 tcatggtcac taccgggatc atcgcgttac gtagcccgtg tttgaggaca acccaggttt
     1561 cgctcacccc tttcgccctc gcggtacgca tataatcttc gcttaaaaca tcgacaaacg
     1621 acgcgcgggt aaagcgcgcc atcacggctg ccactgccgc gccgagcgtc agggagggta
     1681 aaatgtagtg ctgccaactg tctgctccca cggtaggcag ccagcccagt tcaacggaga
     1741 atacctgaat taaaagcatc cccagcgcaa atgccggaaa cgagatcccc gacaccgcaa
     1801 tggtcatact caatcgatcc ggccaacgat tacgccagac ggcggcgata attcccgccg
     1861 ccataccaaa tatcaccgcc cagaccatac tggttatggt cagccacagc gttggcataa
     1921 agcggctggc aatctcatcg gcaaccggac gacgcgacac catcgacagg ccaaaatccc
     1981 cctgcacagc attgctgata tagtgccaga actggtgata cagcggctga tccaacccca
     2041 gctgctgacg caccagttct ataacctgcg catcagcttc gagcccggca atcaatcgcg
     2101 ccggatcgcc gggcagcata tgcacaaata aaaacaccag caccgagacg ataaacagcg
     2161 tcggaatcaa ccccagtaag cgtttgataa cgtaattaag cattccactc cctgcgttgc
     2221 ttattgcaaa tccgcgtctt caaagctgaa gccggtgtct ggcatgatcc aaaaaccggt
     2281 caggttttta ctgtgtgccg acaccagttt ttctaccacc agcgggatcc acggcgactc
     2341 ttgccagatg atatcctgcg ccgccttata caagcgggtc ttttccgccg gatcattagt
     2401 tttcagtgcc tgagccagga agtcgtccac ctgtttattg ctgtaaaatg cggtattaaa
     2461 cagcgtcggt ggccagtttt gcgaggcgaa cagtggcgat agcgcccagt ccgcttcgcc
     2521 ggttgaagcc gaccagccag tgtagaacat ccgcacgccg ctctcttttt gccctttacc
     2581 ttcaacttct gccgcccgct gtccggcatc catcgcggtc acctgggctt taatcccgac
     2641 ctgcgctaac tgctgctggg taaattgcag cactttctgc gcggtgctgt ggttatgtga
     2701 cgaccacagc gtggtactga aaccgttggg atatcccgcc tctttcagta attcgcgcgc
     2761 tttcactgga tcgtaaggcc acggtttata actttgcgcg taggcgatac ttggcggtac
     2821 cacaccagta gctggcgttg catagcccgc aaaggcaact ttcaccagcg ccggacggtt
     2881 aatggcgtaa ttcagcgcct cacggacctt cgggttatcg aacggctttt gcgtcacgtt
     2941 catactgata taacgctgca taattgacgg actggccatc aactcgatat ttttgttttt
     3001 ctccagcagt gtggcctgct cgtagggaat ggggaaagca aactgcgctt caccggtttg
     3061 cagcattgcc gcgcgggtgt tgttatccgc caccggacgc caggttatgc tgtccagttt
     3121 gggcaatcct ggctgccagt aacccgcgaa ttttttcacc ttcacaaaat cggtctgatt
     3181 ccaggtatcc agttcatacg gtccggttcc caccggatga aaaccaatct ccttgccata
     3241 tttttccagc gctgccggtg aaatcatcgc ggtcgccgga tgggcaagaa tattaataaa
     3301 cgctgagaac ggctgtttga gggtaatctt taccgttgtc ggatcgatcg cctccgtttt
     3361 ggcaatattc ttatacaggt tatagcgttt aagatgattc gccggatcgc tggcccggtc
     3421 cagattcgct tttaccgcca ctgcgttgaa atcggtgcca tcctggaatt taatgccttc
     3481 ccgcaatttc acggtgtaag taatgccgtc atcggaaacg gtataactct ccgccagcac
     3541 gtttttcagt ttcatctctt tatccagacc gaacagcccc tggtaaaacg atttcgctac
     3601 ggcctgagat aacgtatcat tcgcgtcata cggatcgagc gtggtgaaat tcgatcctac
     3661 cgccaccacc acatctttgg cagcgaatgc acaagatgcc atcaacgctg tcgcaatgcc
     3721 cagcgccact aacccactac ggtgtacagc tcttgccatt ttattctccg cctgaatgtt
     3781 atctacgcat gaatgcgtat tctgattgtg gttgtgcgac gtaatgcccc ggcccgacgc
     3841 attgcaacga gacggctgcc acctcttcgc cacgcagatg aatattgctg ggaagatcgt
     3901 ccgacagcag tacacgctgc ggtcgttgtc gggacggttc agcgaccgga actgccgcca
     3961 gtaatttacg cgtataagga tgctgcgggt tttcgaagac cgcgcgccgt ggaccaattt
     4021 caacaatttg cccgagatac atcaccgcca cacgatgact aatccgctct accacggcca
     4081 tatcgtggga gataaacaga tacgcaatgc cgaaatcacg ctggagatcg agcaacaagt
     4141 tgataatctg cccgcgaata gaaacatcca gcgccgaaac ggcttcgtcg gcaatgatca
     4201 cttttggatt caatgccaac gcgcgagcaa tgcagatgcg ctggcgctga ccgccggaaa
     4261 actcatgcgg gtaacgccag gcatgttcag gtaacaggcc cacgcgctcc agcaaccacg
     4321 caacgcgtgc agccgcgtct ttacctggca ataaaccgtg tacacgcagc ggttcgataa
     4381 tcgaatcacc gatggtctga cgtgggtcca gcgaagcgta agggtcctga aaaataaact
     4441 gaatatcccg gcgcaatgcc tgaagtttgc cgggtgacaa ggtatcgatt ctctgaccgt
     4501 taaagataat ttcgccgccc tgcgattcga ccaggcgcag caacgcccgc ccggtagtgg
     4561 atttaccgct gccagactcg cccaccagcg atagcgtttc gccaggccag agatcaaaac
     4621 tgactttctc aacggcatgc acttcccgcg ttacgcgatt caacaaaccg ctgcgcaaag
     4681 ggaaacgggt gacaagatta cgcactcgta aaacaggttc gccatccacc accgttttct
     4741 gctcgatggg gggggcctgt ttcgctggat gttcaagcga tatcaacggg aaacgtcggg
     4801 gataatccaa ccctttcatc gcaccaagtt gcggaacagc agctaacagc gcacgggtgt
     4861 aaggatgttg cggtgcatga aaaatctgtt cgacggtacc cgtttccacc gcctcgccct
     4921 gatacatcac cagtacccga tcggcaatct ctgccaccac gcccatatcg tgagtgataa
     4981 agataacgcc catcgacatc tctttttgca atactttgat taattgcagg atctgcgcct
     5041 gaatagtgac atccagcgcg gtggttggct catcggcaat cagcaccgcc gggcggcatg
     5101 acagcgccat cgcaatcatc actcgctggc gcatcccgcc agagagttga tgcggataac
     5161 gtgaaagaat ggtttgtgcc tcaggaatgc gtacctgatc cagcatccgc ttcgcctcga
     5221 ccatcgcttc ttcacgactg gcgttctgat gcagacgaat tgattcggca atctgttcac
     5281 ccacagtaaa taccgggttc agcgatgtca tcggctcctg aaatatcatc gccatatccg
     5341 caccgcgaac atggcgcatt tgtgcagcgc tctgctcgct aagttcaatc acttcgcgac
     5401 tgcgccgctg caacagcatt ttatcgcact gtactaaacc gcccgcctgt tccaacaggc
     5461 gcatcaatgc caacgcagtc actgacttac cggagccgga ttcgccaaca attgccagcg
     5521 tctcaccgcg ttgcagacta aaagagagat tgcggaccgc agctattttc tgctggtcct
     5581 gcataaaggc aatattcaga ttttcaaccg ccagcacatt accggcatca agttcatcac
     5641 tgtgtggcaa cggtgtcccc tttttcacgg tagataccgg tggttggcgt atcgcctgcg
     5701 tagccccagg cgcgatacat tccttcggtg ttaaacggta gcgcgacatt cccttcatgg
     5761 tcgatagcga ttaagccacc gctaccgcca agcgcaggga gtttttccat tactacccgc
     5821 tcgcaggctt ccgcgagact taatccgccg taatccatta acgcggcgat gtcatatgcc
     5881 gccagcgcgc ggatgaagac ttcgcccgtg ccgatacaag aaaccgccac actggcgtta
     5941 ttggcgtagc atccggcacc cactaaggga ctatcgccaa ctcgtccggg taatttattg
     6001 gtcattccgc ctgtggacgt ggctgccgcc aaattgccgt ctaaatccaa cgccacggcc
     6061 cccacggtgc ccattttctg tttttcatcc agtggcgcac cgctatggtc gaggacggtt
     6121 gccccttcct tgcgcgctgc cagtagttgt tcataacgca aaggcgtgga gaaaatctcc
     6181 ggcgagacgc gctccatgcc acgagcaaac gcaaaatttt ctgccccttc gccaatcatc
     6241 atcacatgcg ggctttgctc catcaccagc cgggcggcaa gaaccggatt acgcagatga
     6301 ctaacgcccg ccaccgcacc ggctttcagg gtgttaccat ccatcacaca ggcgtccagt
     6361 tcatgggttt catcacgcgt aaagacagcg ccaattccgg cgttaaacag tggacactct
     6421 tccagcagac gcaccgcttc cgtcaccaca tccagcgcac tttcgcccgc ttccagcatt
     6481 ttctgcccgg tttcaacaat ggcagacaac gcctcaatgt agcgtaattc ctgttgcaga
     6541 ctcatctgcg cgcggctaat tgcacctgcg ccaccatgaa ttgcaatgac tgctttgccc
     6601 atagtattcg tccattaagg gtggctcgcc acttttgcta taaatatcag aatttttatg
     6661 aattactcat tcaatttttg aatatagaaa gatggatatg gcatgtaaag gcaggagcca
     6721 acgctacgcc cgaggtggca tacagtctgt ggcgtttttc tgacataata ggcaaattcg
     6781 attttgcctc cgcaggagtg ttttcatgga atttaccacc ggattgatgt cgctcgacac
     6841 cgcgcttaat gagatgcttt ctcgcgtcac cccactgacc gcccaggaaa cgctgccact
     6901 ggtacagtgt tttggtcgta ttctggcgag cgatgtcgtt tcgccgcttg atgttccggg
     6961 gtttgataac tccgcaatgg acggctacgc ggtgcgttta gccgatattg cctccgggca
     7021 accgctgccc gttgccggta aatcctttgc cggtcagccg tatcatggtg aatggcctgc
     7081 gggcacctgc attcgtatta tgaccggtgc gccggtgccg gaaggctgcg aagcggtggt
     7141 gatgcaggag cagactgaac aaacggacaa tggcgtgcgt tttactgctg aagtgcgtag
     7201 cgggcaaaat attcgccgtc gcggtgaaga tatctctgca ggtgcggttg ttttcccggc
     7261 gggaactcgc ctgactaccg cagagctgcc agtaattgct tcactgggga ttgccgaagt
     7321 tccggtgatt cgtaaagtgc gtgtagcgct tttttctacc ggtgatgaac tccagttgcc
     7381 cggtcagccg ctgggcgacg gccaaatcta cgacaccaac cgtctcgccg tacacctgat
     7441 gttagaacag ctgggttgcg aggtaatcaa cttagggatt atccgcgacg atccccatgc
     7501 cctgcgcgcc gcatttattg aagccgacag ccaggcggat gtagtgatca gttccggcgg
     7561 tgtttcagtg ggtgaagcgg attacaccaa aacgattctt gaagagctgg gggagatcgc
     7621 cttctggaag ctggcgatta aaccaggtaa accgttcgcg ttcggtaaac tcagcaatag
     7681 ctggttctgc ggcctgccgg gcaacccggt ttcagcgacg ctgaccttct atcaactggt
     7741 acagcctttg ctggcaaaac taagcggcaa caccgccagc ggcctgcccg cgcgccagcg
     7801 cgtacgcaca gcgtcccgcc tgaaaaaaac gccaggacgt cttgatttcc agcgcggcgt
     7861 gctgcaacgc aacgccgacg gtgaacttga agtgacgacc accggacatc agggttcaca
     7921 tatatttagc tcctttagtc tcggcaactg ctttatcgtg ctggaacgcg atcgcggcaa
     7981 tgtggacgtg ggcgaatggg tggaagtaga accgtttaac gcgttgttcg gaggcctgta
     8041 atggcggaac tcagcgatca ggagatgctg cgctacaacc ggcaaatcat tctgcgcggt
     8101 tttgattttg acggtcagga ggcgctaaaa gattctcgtg tgctgatcgt cggcctcggt
     8161 ggactcggct gcgcagcttc gctgtatctg gcaagcgccg gtgtcggcaa cctgacgctg
     8221 ctcgacttcg acacggtttc gctctcgaat ctgcaacgcc agacactgca cagtgatgcc
     8281 acggtcgggc aaccgaaggt ggaatccgcc cgtgacgccc tgacgcggat caacccacat
     8341 atcgcgatta cgccagtcaa tgcactgcta gatgacgcag aacttgcagc attgattgct
     8401 gaacacgatc tggtgctcga ctgtacggat aacgttgcgg tacgtaatca actgaacgca
     8461 ggctgttttg ccgcgaaggt gccgctggtt tccggcgcgg caattcgtat ggaagggcaa
     8521 atcacggtct tcacttatca ggacggcgaa ccgtgctatc gctgccttag ccgtttgttt
     8581 ggcgagaacg cattaacctg cgtagaagca ggcgtgatgg caccgttgat cggcgtaatt
     8641 ggttcgttgc aggcaatgga agcgatcaaa ctgctggcag gttatggcaa accgaccagc
     8701 gggaaaatcg tcatgtacga tgcgatgacc tgtcagtttc gcgaaatgaa actgatgcgc
     8761 aatccggggt gtgaggtgtg cgggcagtaa ttgccacctc caggcctgat aagacgcgac
     8821 aagcgtcgca tcaggcattt gctccagacg ccagatgcgg cgtgaacgcc ttatctggcc
     8881 tacggttcgg gcacagattt ccaagcgcgg caggcatcgg tgcactccgc cacgtcatca
     8941 gcaatttatg aggatgcaga acgtccggtt aaatcgacgt tctgccaaac gctccctgcc
     9001 agtcctgctc aaacttcgcc acagcggctt ccaccgccgg atagctaatc atctgttgtg
     9061 ccacatccag tggcagagta attgattcac atcctgccag taagcagtcc agcgcctgac
     9121 gcggggtttt gaaactcgct gccagcactt tcgcctgcgg cgcatgcatt ttcaataact
     9181 ggtgtaagtc ggtcacagtc tgaatgccgc taccgccctg agcatcaata cgattaacgt
     9241 aaggcgcaac atattccgca cctgccagcg ccgacagcaa cccttgtgct gcgccatata
     9301 ccgcggttcc cagcgttgga atcccttccg cttttaacat cttaatagct gccagcccct
     9361 cggcggtcac cggaactttc accacgatat ccgcaataat agaacgcaat ttacgtgcgt
     9421 cattcaccat cccttcagca gtggtagcca ttacctgggc aaacagacgc ccctgaccgc
     9481 ccatcgcttc atgaagttgc ggaagcacaa catccagcgg ttttttaccc gcggcgataa
     9541 tgcttgggtt agtggtcaca cccgccagcg gaaaaatacg tgacagcgcc ttcaccgcaa
     9601 caacgtctga agtatccaga tacagttcca taaccatcct caaaatgtac tttttgtctg
     9661 aatgtagact atcacgggaa aactctctga acagttgacg cacatcaatc taactttcat
     9721 tcgaaagtat tttaatgttt gaacgaaaca gagaggcgac tatgattttc aatattcagc
     9781 gctactcgac ccatgatggc cccggtatcc gcacggtcgt atttcttaaa ggctgttcgc
     9841 tgggctgccg ctggtgtcag aacccggaaa gccgcgcccg cacgcaggat ctgctgtatg
     9901 acgcacgact gtgtctggaa ggctgcgagc tgtgcgctaa ggccgcgccg gaagtgattg
     9961 agcgcgcgct gaatggtttg cttattcatc gggaaaagtt aaccccggag catctgacgg
    10021 cgttaaccga ctgctgtccg acacaggcat taaccgtgtg tggtgaagtg aaaagcgttg
    10081 aggagatcat gacgaccgtt ctgcgcgata aaccgtttta cgatcgcagc ggcggcggtt
    10141 taacgctttc gggtggtgag ccctttatgc agccggaaat ggcgatggcg ctactgcaag
    10201 ccagccacga ggcaggcatt catactgcgg tagaaacctg tctgcatgtg ccgtggaaat
    10261 atatcgcccc ttctctgccc tatatcgatc tgtttcttgc cgatttaaaa cacgttgccg
    10321 acgcgccgtt taaacagtgg accgacggta acgccgccag agtgctggat aacctgaaaa
    10381 aactcgccgc agcgggcaaa aaaatcatta tccgcgtgcc gctgattcag ggctttaatg
    10441 ccgacgaaac ctctgtaaaa gctattaccg attttgccgc cgacgagctg cacgttggcg
    10501 aaattcattt cctgccctac cacacgctag gcatcaacaa atatcactta cttaatctgc
    10561 cctatgacgc cccggaaaaa ccgcttgatg cgccagaact gctcgacttt gcccagcagt
    10621 atgcctgcca gaaagggtta accgcgacct tacgaggata acaaccatga ccacactgaa
    10681 actggacacg ctcagcgacc gcattaaagc gcacaaaaat gcgctggtgc atattgtgaa
    10741 accgccagtc tgtaccgagc gcgcgcagca ctataccgag atgtatcaac aacatctcga
    10801 taagccgatc ccggtacgtc gcgcgctggc actggcgcat cacctggcga atcgcaccat
    10861 ctggatcaaa cacgatgagt tgatcattgg caaccaggca agcgaagttc gcgccgcgcc
    10921 gatcttcccg gaatatactg tctcgtggat cgaaaaagag attgatgatc tggcagatcg
    10981 tcccggtgct ggctttgcgg tgagcgaaga gaacaaacgc gttctgcatg aagtgtgccc
    11041 gtggtggcgc ggtcagaccg tacaggatcg ctgctacggc atgtttaccg atgagcaaaa
    11101 aggtctgctg gcgaccggaa tcattaaagc ggaaggcaat atgacctccg gcgatgcgca
    11161 cctggcggtg aatttcccgc tgctgctgga aaaaggtctt gatggtctgc gcgagaaagt
    11221 agcggaacgt cgctcgcgca tcaatctgac ggtactggaa gatttgcacg gcgaacagtt
    11281 cctgaaagcg attgatatcg tgctggtggc agtcagcgaa cacattgaac gttttgcagc
    11341 cctggcgcgt gaaatggccg cgaccgaaac ccgcgaaagc cgtcgcgatg aactgctggc
    11401 gatagcagaa aactgcgatc ttatcgccca ccagccgccg cagactttct ggcaggcgct
    11461 gcaactgtgt tacttcatcc aattgatatt gcagatcgaa tctaacggtc actcggtatc
    11521 gtttggtcgt atggaccagt atctctaccc gtactatcgc cgcgacgttg aactcaacca
    11581 gacactggat cgcgaacacg ccatcgaatt gctgcatagc tgctggctga aactgctgga
    11641 agtgaacaag atccgctccg gctcacactc aaaagcctct gcgggaagtc ctctgtatca
    11701 gaacgtcacc attggcggac aaactctggt tgatggtcaa ccaatggacg cggtgaatcc
    11761 actctcttac gctatcctcg aatcctgcgg tcgcctgcgt tccactcagc ctaacctcag
    11821 cgtgcgttac catgcaggaa tgagtaatga tttcctcgac gcctgcgtac aggtgatccg
    11881 ttgcggcttc gggatgccgg cgttcaacaa cgacgaaatc gtgatcccgg aatttattaa
    11941 actcggtatt gaaccgcagg acgcttacga ctacgcagcg attggttgta tcgaaaccgc
    12001 cgtcggtggc aaatggggct atcgctgtac cggcatgagc tttatcaact tcgcccgcgt
    12061 gatgctggcg gcgctggaag gcgggcgtga tgccaccagc ggcaaagtgt tcctgccaca
    12121 agaaaaagcg ttgtcggcag gcaactttaa caatttcgat gaagtaatgg acgcgtggga
    12181 tacgcaaatc cgttattaca cgcgtaaatc aatcgaaatc gaatatgtcg tcgacaccat
    12241 gctggaagag aacgtgcacg atattctctg ctcggcgctg gtggatgact gtattgagcg
    12301 agcgaaaagt atcaagcaag gcggcgcgaa atatgactgg gtttctggcc tgcaggttgg
    12361 cattgccaac ctcggcaaca gcctggcggc agtgaagaaa ctggtatttg aacagggcgc
    12421 gattggtcag caacagctgg ctgccgcact ggcggatgac ttcgatggcc tgactcatga
    12481 gcaactgcgt cagcggctga ttaacggtgc gccgaagtac ggcaacgacg atgataccgt
    12541 cgatacgctg ctggctcgcg cttatcagac ctatatcgac gaactgaaac agtaccataa
    12601 cccgcgctac ggtcgtggtc cggttggcgg caactattac gcgggtacct cgtctatctc
    12661 cgctaacgta ccgtttggcg cgcaaactat ggcaacaccg gacgggcgta aagcccacac
    12721 cccgctggca gaaggcgcaa gcccggcctc cggtactgac catcttggcc ctactgcggt
    12781 cattggctca gtgggtaaac tgcctacggc agcgattctc ggcggcgtgt tgctcaacca
    12841 gaaactgaat ccggcaacgc tggagaacga atctgacaag cagaaactga tgatcctgct
    12901 gcgtaccttc tttgaagtgc ataaaggctg gcatattcag tacaacatcg tttcccgcga
    12961 aacgctgctg gaggcgaaaa aacatccgga tcagtaccgc gatctggtag tgcgtgtcgc
    13021 gggctattcc gccttcttca ccgcgctctc tccagacgca caggacgata tcatcgcccg
    13081 tactgaacat atgctgtaat ctcctcggcc cggcggcgtc gtcgccgggc taaatcattc
    13141 acatcatcaa tttcatcctt actttcattc gaaatataat ttgtgctctg cgtcacattg
    13201 ttattacact gttacggttt cattttcgag ccaggagcac acctatgagc gtaaaagtta
    13261 tcgtcacaga catggacggt acttttctta acgacgccaa aacgtacaac caaccacgtt
    13321 ttatggcgca atatcaggaa ctgaaaaagc gcggcattaa gttcgttgtc gccagcggta
    13381 atcagtatta ccagcttatt tcgttctttc ctgagctaaa ggatgagatc tcttttgtcg
    13441 cggaaaacgg cgcactggtt tacgaacatg gcaagcaact gttccacggc gaactgaccc
    13501 gacatgaatc gcggattgtt attggcgagt tgctaaaaga taagcaactc aattttgtcg
    13561 cctgcggtct gcaaagtgca tatgtcagcg aaaatgcccc cgaagcattt gtcgcactga
    13621 tggcaaaaca ctaccatcgc ctgaaagctg taaaagatta tcaggagatt gacgacgtac
    13681 tgttcaagtt ttcgctcaac ctgccagatg aacaaatccc gttagtgatc gacaaactgc
    13741 acatagcgct cgatggcatt atgaaacccg tcaccagtgg ttttggcttt atcgacctga
    13801 ttattcccgg tctacataaa gcaaacggta tttcgcggtt actgaaacgc tgggatctgt
    13861 caccgcaaaa tgtggtagcg attggcgaca gcggtaacga tgcggagatg ctgaaaatgg
    13921 cgcgttattc ctttgcgatg ggcaatgctg cggaaaacat taaacaaatc gcccgttacg
    13981 ctaccgatga taataatcat gaaggcgcgc tgaatgtgat tcaggcggtg ctggataaca
    14041 catccccttt taacagctga cctctctacc ggagccgtgc gctccggttt tcccttcttt
    14101 ttacatcctg tacatcacat caaatcatta aacttgcatt aacttattta taacttatat
    14161 tgtcgcatgc aaatcagaat ggtttcgaat gaaagttaat gagaggtggt tatggcttct
    14221 acttttacca gcgacacatt gcctgccgat cacaaagcag ctatccgtca gatgaagcac
    14281 gcgctgcggg cgcagcttgg cgacgtccag cagatcttta atcagctaag cgatgacatt
    14341 gccacgcgag tggctgaaat caacgcactc aaagcacagg gcgatgccgt ctggccggtg
    14401 ctgtcttatg ccgatatcaa agcaggtcat gttactgcag agcagcgcga acagattaaa
    14461 cgtcgcggtt gtgcggtgat aaaaggccat ttcccccgcg aacaagcgct aggctgggat
    14521 cagtcgatgc tggactatct ggaccgcaac cgctttgacg aggtctacaa aggccccggc
    14581 gataatttct tcgggacgct cagcgcttca cgtcccgaga tttaccccat ctactggtcg
    14641 caggcgcaaa tgcaggcccg ccagagtgaa gaaatggcga atgcgcagtc gtttctcaat
    14701 cgtctgtgga catttgaaag tgatggaaag caatggttta acccggatgt gagcgtcatc
    14761 taccctgacc gtatccgccg ccgtccgccc ggaacgacct ccaaaggtct tggagcgcat
    14821 accgactccg gggcgctgga acgctggctg cttccagcgt atcagcgcgt tttcgccaac
    14881 gtctttaatg gcaatctggc gcaatatgat ccctggcatg cggcacatcg tacggaagtt
    14941 gaagagtaca cggtggacaa caccaccaaa tgttccgtgt ttcggacatt ccaaggctgg
    15001 acagcgctct ctaatatgct gcctggtcag gggctgctgc acgttgtgcc cattcctgaa
    15061 gccatggcgt acgtactgtt acgtccgctg cttgatgatg tgccggagga tgaactgtgc
    15121 ggcgtagcgc ccggaagagt gttgccggta tcagagcaat ggcatccact gttaattgag
    15181 gcgttaacca gcattccaaa actcgaggcc ggagactccg tctggtggca ctgcgacgtc
    15241 atccattccg ttgcccccgt tgaaaatcaa cagggttggg gcaacgtgat gtacattcct
    15301 gcggcaccaa tgtgcgagaa aaatcttgcc tacgcgcaca aggtgaaggc cgcactggaa
    15361 aaaggcgcat cgccgggcga cttcccgcgc gaggactatg aaacaaactg ggaaggacgc
    15421 tttacgcttg ccgacctcaa cattcacggt aagcgagcgt tgggaatcga cagctgagtg
    15481 tcgtacagcc cttcccgttc cacggcggtg ttcccttttc ctgggcgaac ccgccgcact
    15541 gactcccgca ccgccaatcg tccgtgcagc agcagcgagc ccaccggagc atccgggcga
    15601 atcagccgct gttgcaacat ttgcaccgct tcattgccta gctcatcgcg tggcacatgc
    15661 acagccgtca ggggaacatc ctcgattgcc gcaaggttaa agccgtcgat actcatcacc
    15721 gagacatcct gtggtacgcg cagaccacga cgatgtagcg catttaccgt tcccgccgcc
    15781 ataaaatccc cgccgaccag aaacgccgtc ggcaacgtct tgccattggc ggcatccagc
    15841 cacgctccga ccttttcttc cgcttcttta gcgctaaaac tgctgatatt cagcaaatga
    15901 cggtcatggt gaaaggccag attatgctga cgccatgatt ctttaatccc tgcaaaccgc
    15961 tgctccatag tgtagcggcg caggcacatc acattcatca cctcccggtg acccatttca
    16021 aacagatagc gggcggcgaa cgcgccaatt aaccgatgat ccggcgcaat cgccgggagg
    16081 cgcattcgtt catcacggca gttgatcagc acacaaggct tagcgagatc ggcggccaga
    16141 tcatggatgt gcggatcgtc aatccccagc aggatcgctg cctgcgtatc tgcttcattc
    16201 atccgcgaga gaaagagcgc gggatcgctg tcaaactcct ccagcgcgca gtaacgcagc
    16261 cgcacttcat gcggcgataa cgctttgctg atgctctgga tcacacggta ataaaagata
    16321 tcagatcgtt ggtcaaaagc gcgttgcggc gcgaaaataa tcaggttgtt cagcaacata
    16381 cgacctgctg ccatgccatc catcacgccc aactcacgcg cgcaggccag cacctgttcg
    16441 cgagcgcgtg cactggtatt cgctttgcca gccagcaccc gcgaaaccgt actcggcgat
    16501 agcccggtac gtgcggcgat atcggcgatt ttcagcttct tgtccatttt gtgatctgcc
    16561 tctaattcgg aaatgacaga attttcatga gcaaaaaagc ccattctgtc tggcattaaa
    16621 tccatttcag taatgtatat cagtcactca tggaaaaacg tgcacaaaac ccgctttctt
    16681 tcgcttttag ggtctcttac tgtgaattgg tagaacaact tccatacaaa aataatggaa
    16741 aaacgaacaa taaagacttt atttatcaaa aagttaaaag tggtgtaaca actccattct
    16801 gacgttgccg cacaaaccct ataccattct gtggagtgca tatgagtcag ggtatcaata
    16861 acgatatgac tgccagtaaa ccgcgccata tcgtcaaaaa tctgcgttgg tggatactgg
    16921 tccttttttt gctcggtgtt actgtcaact acatcacgcg taactcgctg ggtattctcg
    16981 cccccgaact gaaaagcagc ctagggatta ccaccgaaca atattcctgg attgtaggtg
    17041 ctttccagct cgcctatacc ctattccaac cgttgtgcgg atggctgatt gacgtcattg
    17101 gcctcaaact gggctttatg atctgcgcgg gactgtgggc actggcctgt ctgatgcatg
    17161 cgggggcggg aaactgggga catctggcgc tgttgcgctt ctttatgggc ggtgcggaag
    17221 ccgcggcaac cccagcaaac gccaaaacca tcggtgaatg gtttccaaaa tcagaacgcc
    17281 cgatcgccgc aggctgggca ggcgtcggtt tttccatcgg tgccatgctg gccccaccga
    17341 ttatctattt tgcccacgct tcattcggct ggcagggtgc atttatgttc actggcatcc
    17401 tggcgatgct gtgggtggtg ctgtggtggg cgttctatca cagcccggat aagcatccta
    17461 acctcagcaa agccgagctg gactttattc gccaggataa cgaagcgccc ccggtcaagt
    17521 taccctttct gaccgcactg aaaaccgtgg gcaaaaataa acgcttttac ggtatcgcta
    17581 tcccggcctt tatggcagaa ccggcgtggg cggtgatgag tttctgggtg cctctatacc
    17641 tgtccaaaac ctatggcatg gacctgaagc agatcgcatt attcgcctgg ttgccgttcc
    17701 tcgccgctga tcttggcagt attgccagcg gctatctgac caaactttac acccgcctgt
    17761 ttgggctgag tcgtgtgaac tccgttgtcg catcatcggt cagtggcgca ttcttgatgg
    17821 tttcgctggc ggtgatggcc tttaccaata acccgtatat cgccatcgtc cttatctcca
    17881 tcggcggctt cggtcaccag atcatctcct gcatgctgag cgcactggtg gtggaatcct
    17941 tcgacaaagg tcagatggcc actgttaacg gcatgcgcgg ttctgccgcg tggatcgcaa
    18001 gtttcctgtt ctccctgatt atcggggtta ccgctgacaa aatcggcttt aaccccctgt
    18061 ttatcgccat gggcttcttt gacctgattg gcgctttctt cctggtcacc tttattgctg
    18121 aacgtcgcgc aaaacgcgcc tgagagtgga tgtaaacatg aaaacgctga aaaactggac
    18181 cctgcacaaa cagtctgccc accacgttga actgctggtc gatggccagc atatcctgtg
    18241 cctgtacgtg ctggaagaaa atctgttccg cgtgctgtta aaacgcaaag gcgagctggc
    18301 gctagatcgc acctggagca ttgctccggc gcaggatgtg ccatgggaag ggcgcagccg
    18361 cgaagatctc agcggcttta gcctgcccgc gtggacagtg atccagcacg acgacaccgt
    18421 ggttatcgcc accgagtcac tgcgtgcaac cgttcaccag ccgctgtggc tggagtggca
    18481 ctatcgtaac aacgaaggcg aatggctgcc gttggtgaat gaccgcccga ccagcgctta
    18541 tctgctgaat gcccacggcg atggcgtggc acactatcag agccgtcgta aagatgaacg
    18601 cttttatggc ctcggagata aagccggcga tctgcaacgc aacggtaaac gttacgagat
    18661 gcgtaacctc gacgcgatgg gctacaacgc cgttagtact gacccgctgt acaaacacat
    18721 tccattcacc atcacccgcc gcgatgacat tagcttcggc ctgttttatg acaacctcag
    18781 cagctgctgg ctggatctgg gcaacgagat cgacaactac cacaccgcct atcgccgctg
    18841 gcaggcggaa gcgggtgata tcgactacta cctgtttacc ggcgaacgcg tacttgatat
    18901 caccaaagcc ttcgtacgcc tgaccggaaa aaccctgttc ggaccgaaat ggagcctggg
    18961 ctacagcggt tcaactatgc actacaccga tgcgccggac gcacaaaatc agttgatgaa
    19021 ctttatccgc ctgtgtgaag aacatgcaat tccctgcgat tcgttccagc tctcttccgg
    19081 ttatacctca attaacggca aacgctatgt gttcaactgg aactacgaca aagtgccgca
    19141 gccgaaagtg atgagccagg catttcacaa tgctggtttg cacctggccg ccaatatcaa
    19201 gccgtgcctg ttgcaggatc acccacgcta taacgaagtg gcagaaagag gactgttcat
    19261 ccgtgattca gaaacagatg aaccagaacg ttccagcttc tgggatgacg aaggttctaa
    19321 cctcgagttt actaatccgc agaccatcca gtggtggcag gaaggcgtga ccacacagct
    19381 tctggagatg ggtatcgact ccacctggaa cgacaacaac gaattcgaag tatgggacgg
    19441 tgaagcacgc tgtcacggct ttggtcagac gatcgcgatc aaacatattc gcccagtgat
    19501 gccactgctg atgatgcgag cctcgatgga agcccagcag cgtttcgctc cggaaaaacg
    19561 tccgtacttg atctcccgct ccggctgcgc gggaatgcag cgctacgtgc agacctggag
    19621 tggcgataac cgcactagct gggacactct tcgctacaac acccgcatgg gtctggggat
    19681 gagcctctcc gggctgtata acctcggtca cgacgtcggc ggtttctccg gtgataagcc
    19741 ggacccggaa ctgtttgtac gctgggtaca aaacggcgtg atgcatccgc gctttacgat
    19801 tcactcatgg aacgatgacc ataccgtaaa cgaaccgtgg atgtatccgg gcgtcactcc
    19861 ggcgatccgc agcgctattg agttgcgcta tcgcctgctg ccctactttt ataccctgat
    19921 gtggcaggca tacgccgatg atgaaccaat gctacgtccg actttccttg accacgagca
    19981 cgacgttcag acttttgaag agtgtgatga cttcctgctt ggtcgcgata ttttggttgc
    20041 cagcgtcgta gagcaaggcg agcgccagcg ccgtgtatgg ctgccagata acgaaactgg
    20101 ctggtacgat ttctacaacg gcgaatggtt cagtggcggt cagtggatca ccctcgacgc
    20161 accgctggag aaactgccgc tgatggttcg tgccggggct ggtctaccgc tcagcaaacg
    20221 catcacctat gtgagcgcag aacaagacga tacccgcgaa ctgaaactgt tcccgctgaa
    20281 aggtgtgggc acaacgtccg gcctgctgtt tgaagacgat ggcgaaagct ggggctacca
    20341 aactggtaat gcgctttggg tggaatggga aatggtttgc gatggggcga ccatcaacct
    20401 gaaagtcaac gcgcgcgggg attatcgtcc tgcatggagt gcactgaaag tgtcattacc
    20461 attggaagaa aaacgcacgc tgctggtcaa tggtgttgaa gggagtgaat ggatgcgtta
    20521 aatcattacc ggggccggga ggcgactgca ccttacccgg tccagccatc gaatccttta
    20581 cctcagtatt tcacgctgca ggaaggcagc aaacctgtaa accccgggag cttaaataag
    20641 taagtgaccg gggtgaacag gtgaagccaa tgcatctgcc gcatgaaaga cggcggggaa
    20701 ttaagcgtcg atccctttac tacgcaggta atcttcgtaa ttaccgctaa agtcgatcgc
    20761 gcgttccggg gtgatttcca gaatgcgggt cgccagggag cttacgaact cacggtcgtg
    20821 tgaaacaaag atcagcgtgc cctgatacag ttccagcgcc atgttcagtg actcgatgga
    20881 ttccatatcc aggtggttgg tcggttcgtc catgatcaga atgttcggct tctgcatcat
    20941 taacttacca aacagcatcc gccctttttc cccaccggaa agcactttag ctggcttttt
    21001 gatgtcgtcc tggctgaaca gcaaacgacc gagaatgctg cgtaccgcct gctcgtcatc
    21061 gccttcctgc ttccactgac tcatccattc gaacacggtc agatcatttt caaactcata
    21121 ttcgtggtcc tgggcgtagt aaccaatgcg cgcgttttca gaccatttta cggtgccgct
    21181 gtccggttgc agatcgccca ccagcgtttt cagcagcgtt gatttaccga cgccgttggt
    21241 acccagtacc gcaagttttt caccgacctc cagcagcagg ttgagatttt taaacagtgg
    21301 accgttatca aaccctttgg tcagaccttc cacttccagc gcgttacgga acagtttctt
    21361 atcctgttca aaacggatga acgggttctg acggctggag gctttcacct cttccagttt
    21421 gattttatca atctggcgcg cgcgcgaagt tgcctggcga gatttcgagg cgttggcgct
    21481 aaagcggcta acgaaagatt gcaactcagc aatctgcgct ttcttcttgg cgttatcggc
    21541 cagcagacgt tcacgcgcct gggtcgccgc cgtcatgtac tcatcgtagt tacccggata
    21601 aacgcgcagc tcgccgtaat ccagatccgc catgtgggta cagaccatgt taaggaagtg
    21661 acggtcgtgc gagatgatga tcatggtgct gtcacgctcg ttcagcacct gttccagcca
    21721 gcgaatggtg tcgatgtcga ggttgttggt cggttcgtcg agcaggagaa tctccggatc
    21781 agcaaacagc gcctgcgcca gaagcacacg cagtttccag ccaggagcaa cttcactcat
    21841 cgggccgtag tgctgttcca ctggaattcc cacgccaagc aacagttcac cggcgcgagc
    21901 ttccgcagag taaccgtcca tttcgccgta tttaacttcc agatcggcca ctttatagcc
    21961 gtcttcctca ctcatttccg gcaaagcata gatgcggtca cgctcctgct tcacttccca
    22021 caactcttta tgccccatga tcaccgtatc cagcacagtg aactcttcaa aggcaaactg
    22081 atcctgacgc agcttaccaa tgcgctcgtt gggatcgagg gaaacgttac ccaaagtcgg
    22141 ctctaagtcg ccgccgagga tcttcataaa ggtggattta ccactaccgt tcgcgccaat
    22201 caggccgtaa cggttgccgc cgccgaattt gacggaaatg ttttcaaaca acggcttact
    22261 gccgaactgc atggtgacgt tactggaaac taacacaggc gtatcctgaa aagagatatg
    22321 acaaaccgcg cattatgcca taagtctgaa agaagatcct gctcctcgct taaggtctac
    22381 actcaagcca aaggcaaaaa atgtgatttc gtacacatct gatttcactg tgagctggaa
    22441 tgaacttata atgcgcttcc aatactctaa tattctcaac ccaatggcct gccaggcaca
    22501 aaatctcgct taacatgaat atgaaattga aaacattatt cgcagcggcc ttcgctgttg
    22561 tcggcttttg cagtaccgcc tctgcggtaa cttatcctct gccaaccgac gggagtcgcc
    22621 tggttggtca gaatcaggtg atcaccattc ctgaaggtaa cactcagccg ctggagtatt
    22681 ttgccgcgga gtaccagatg gggctttcca atatgatgga agcgaacccg ggtgtggata
    22741 ccttcctgcc gaaaggcggt actgtactga acattccgca gcagctgatc ctgccggata
    22801 ccgttcatga aggcatcgtc attaacagtg cagagatgcg tctgtattac tatccgaaag
    22861 ggaccaacac cgttatcgtg ctgccgatcg gcattggtca gttaggcaaa gatacgccta
    22921 tcaactggac caccaaagtt gagcgtaaga aagcaggccc gacctggacg ccgaccgcca
    22981 aaatgcacgc agagtaccgc gctgcgggcg aaccgcttcc ggctgtcgtt ccggcaggtc
    23041 cggataaccc gatggggctg tatgcactct acatcggtcg cctgtacgct atccatggca
    23101 ccaacgccaa cttcggtatc ggcctgcgtg taagtcatgg ttgtgtgcgt ctgcgtaacg
    23161 aagacatcaa attcctgttc gagaaagtac cggtcggtac ccgcgtacag tttattgatg
    23221 agccggtaaa agcgaccacc gagccagacg gcagccgtta tattgaagtc cataacccgc
    23281 tgtctaccac cgaagcccag tttgaaggtc aggaaattgt gccaattacc ctgacgaaga
    23341 gcgtgcaaac agtgaccggt cagccagatg ttgaccaggt tgttcttgat gaagcgatta
    23401 aaaaccgctc cgggatgccg gttcgtctga attaatcttc aaaacttaaa gcaaaaggcg
    23461 gactcataat ccgccttttt tatttgccag accctagttg gccgggagta taactaacaa
    23521 aacatatccc accaacgccg cccacaacag catcggtatt gaatagagat ggaaacgcca
    23581 ccagatgcgg cgatcgttcg ccatacgtag cgcaatcaaa tttgccagcg atccgggtaa
    23641 taacccaaag ccacctacgt ttaccgccca taccagtaat aaagacggcg gcacatagtt
    23701 cagcaacaat atggtactcg gcacgttact gatcacctgc gataaaccga ttgccgttaa
    23761 ccataacccg ggttcagata gatgactcac gttacccaac acgccttgca acgctggaag
    23821 ctgggtcagt aaatggacgt cgataaacat cgccataaac accagcagca gcgtccagtc
    23881 cacactgagc accacgcgac gtgccagcag cgcaaagcct gccgccacaa tcaccagtcc
    23941 ccacagctct tgtttgaact ccagcgccgt cagaaagacg atatacagcc ccagacaact
    24001 ccacaccagc cgcggtttcc actccggtgt ttgcaccccc gtatggtatt gcagcgcctt
    24061 tccagggaaa caacaccagc acaggagcag gagcgtcagc atcattgcgc cagccagcgg
    24121 tgccatttgg gcaataaatc cggcaaacga aagaccagaa cgtccccaga taagaatatt
    24181 ttgcgggttg ccaattggcg tcagtagcga accagcgttg actgccagcg cctcaaaaat
    24241 aatcagccga ttaaccggga tctcacacag tctttttagc gtgatagtca gcggaacaac
    24301 aataaacagc gcgacatcgt tggtcagaaa ggtagaaagc agcgccgccg ccagcaccat
    24361 aaacatcgcc agccgacgct ccgtagcaaa gcggcgcacc attttgcgcc ccagcacatc
    24421 aaaataaccg cttaactcca cacctttggt cagcagcatc aggccgctta aggtgatgat
    24481 ggtgtgccag tcgatagcag caggccagga tttcggtgca aagggcacga aaaagcttaa
    24541 tccgatacca acaagaatta ataactgaaa aaaacgatcg ccttgcagcg tgcgtaaaaa
    24601 aggcaggctc atttggcacc gtgtttctgg gtaaacaaac gaaaagcgtc gagcgtctct
    24661 tcactaacat ggtgctccat gccttccgcg tcgcgacggg cgatttccgg actgacgccc
    24721 aacaccaaca agaaattttc gactatctga tgtcgctcgc ggctttcctg cgccagcttc
    24781 tctccttctg ccgttaaaaa cacgccacgc caggggatca tttcaatcag ccccatggtt
    24841 gccagccgct taagcatttt agccaccgtc ggttgcgaaa ctcccagacg agcagccatg
    24901 tccacctgac gagcttcccc cacttccctg atcaagtcag aaatcagctc aacgtaatca
    24961 tcaataagct cgcgccgatg cgcctctctg acctggcgga acccttcaac gtgctcttcc
    25021 acgttcacta actgcgtcac tttttttgct gttggcgtac ctgcgcgacg actcattgtg
    25081 cttcctcatt atggtgacgc atacatagcg ttctcatttc gtgcgcacat tgtaaaccag
    25141 agttgcgaag gtacaaaaaa ttaacgtttt agcaatagct acataatata gcctgtgcta
    25201 tatctgtatg taatgcaatc atccctcaag gatcgacggg attagcaagt caggaggtct
    25261 tatgaatgag ttcaagaagt gtatgcgcgt gtttagtcat tctcccttta aagtacggtt
    25321 aatgctgctc tctatgttgt gcgatatggt caacaacaaa ccgcagcaag ataaaccttc
    25381 cgataaatag cggcgtcgcg gtacgccgct tcactcctgc ttttatgcag gcataacgcg
    25441 ttttggtctg aaaaacccca ctttttgtcg gatttgcaat ccccttcgca aaagatttgt
    25501 tcgtcagtag ttgacctgaa cggcggctcg ctctatcttc ttgcagccct acgtatattg
    25561 cggctcgcgg atgcggaccc ctttcccctc ttcacgcact cttgcaggta ttgacccttg
    25621 acgccagggt aagcacatgg cgtttgttac gatagtggca tatgaattta accctcaaag
    25681 aatcgcttgt tacccgtagc cgggtattta gcccgtggac tgcgttctac tttttacagt
    25741 cgctattaat taacctcggc ttaggttacc ccttcagttt gctctacacc gctgcgttta
    25801 cggctatttt gcttttgcta tggcgaacat tgcctcgcgt acaaaaagtt ctggtcggtg
    25861 tcagttcgct ggtggcggct tgttatttcc cttttgctca ggcctacggc gcgcctaatt
    25921 tcaatacatt gctggcattg cactccacca atatggaaga gtcgaccgaa atcctgacga
    25981 tttttccgtg gtacagctac ctggtcggct tatttatttt tgcgctcggc gtaatagcaa
    26041 tcaggcgaaa aaaagagaat gaaaaagcgc gctggaatac cttcgacagc ctgtgtctgg
    26101 tattcagtgt ggcgacattt tttgttgctc ccgtgcaaaa cctggcctgg ggtggcgtat
    26161 ttaaactgaa agatactggc tatccggtat ttcgttttgc taaggatgtc atcgtcaata
    26221 ataacgaggt gattgaagag caagaacgga tggcaaaact ttccggaatg aaagatacct
    26281 ggacggtcac tgccgttaag ccgaagtatc agacctatgt ggtggtgatc ggcgaaagcg
    26341 cgcgtcgcga tgccctcggt gcctttggcg gtcactggga caataccccg tttgccagca
    26401 gcgttaacgg tttgatattt gctgactaca ttgccgccag tggctccacg cagaaatcac
    26461 ttggcttaac gctcaatcgc gtagtcgatg gcaaaccaca gtttcaggat aactttgtca
    26521 ccctggcaaa tcgcgcgggc ttccagacct ggtggttttc caaccagggc caaatcggcg
    26581 aatacgatac tgctatcgcc agcatcgcca aacgtgcaga tgaagtgtac ttcctgaaag
    26641 aaggtaattt cgaagcagat aaaaacacga aagacgaagc gttactggat atgaccgctc
    26701 aagtgctggc gcaagagcac tcgcaaccgc agctgattgt tctgcatctg atgggctcgc
    26761 atccgcaggc ctgcgacagg acacaaggca aatacgaaac ctttgtgcaa tcgaaagaaa
    26821 cgtcgtgcta tctctatacc atgacgcaaa cggacgattt actgcgcaag ctgtacgatc
    26881 agttacgcaa cagcggcagc agcttctctc tggtttactt ttctgaccac ggtctggcct
    26941 ttaaagagcg cggcaaagac gtgcaatacc ttgcccatga tgataaatat cagcaaaatt
    27001 tccaggtgcc ttttatggtc atttccagcg acgataaagc gcatcgggtg attaaagccc
    27061 gccgctcagc caatgacttc ttaggctttt tctcgcagtg gacgggaatt aaagcgaagg
    27121 aaataaatat caaatacccg tttatatctg agaagaaagc cgggccgata tacatcacca
    27181 acttccagtt acagaaggtg gattacaacc atctcggaac cgatattttc gacccgaaac
    27241 cttaaaacaa aaaatccgcc ccgagaggcg gattttttat atcaccaaag tgattagaag
    27301 cggtaaccaa caccggcaat ccaggtgcct acgtcaacgc tacgaatacg gctctgctcg
    27361 taagagaagt ccagagcaac gttttccatc gggttgaact gcagaccagc accgtaggag
    27421 aaaccgtagt cgctggtgtc gtgtttgtag gtcgggtatt cagtggtctg gaatttacca
    27481 taacccaccc ccactacacc gtagatgctt gcccagtcgt taatgcggta agccggacca
    27541 gcagtgatgc cgtagtactg gtttttgttg tagtcaccag agcttgcagt acggcttttc
    27601 tcggtgtaag tgaaagaacc gatcacaccc agcgggctgt tgtcttcttc atagcggtat
    27661 ttcaggttga aaccgcccat tttgttcatt tggccctgag cgtcgctctg tgcgtaaccg
    27721 ccagttacgg tagaagtcgc agctacggaa gtacctgcgg tgaaagccag aactgcggcc
    27781 agtgctgaaa gacatgcaat ttttttcata accacctcaa atgtgattca aataagtcct
    27841 aagttttaaa tatatcaaaa attaatggga aactcttcgc gatttgtgat gtctaacggg
    27901 ccatttcatg taacagaacg tttccataca ccgctatcca tctaaattta aatcactttt
    27961 tcagagaact gcgtaagtat tacgcatgtt ttccctgtca ttcatccaga ttattcctaa
    28021 tcaccagact aatgattcca tcaatcctgg cgcattttag tcaaaacggg ggaaaatttt
    28081 ttcaacaaat gctcaaccag cattgggtat atccagtaca ctccacgctt tacttaagtc
    28141 tagatatttg tgggagaaag gatgcctggt tcattacgta aaatgccggt ctggttacca
    28201 atagtcatat tgctcgttgc catggcgtct attcagggtg gagcctcgtt agctaagtca
    28261 ctttttcctc tggtgggcgc accgggtgtc actgcgctgc gtctggcatt aggaacgctg
    28321 atcctcatcg cgttctttaa gccatggcga ctgcgctttg ccaaagagca acggttaccg
    28381 ctgttgtttt acggcgtttc gctgggtggg atgaattatc ttttttatct ttctattcag
    28441 acagtaccgc tgggtattgc ggtggcgctg gagttcaccg gaccactggc ggtggcgctg
    28501 ttctcttctc gtcgcccggt agatttcgtc tgggttgtgc tggcggttct tggtctgtgg
    28561 ttcctgctac cgctggggca agacgtttcc catgtcgatt taaccggctg tgcgctggca
    28621 ctgggggccg gggcttgttg ggctatttac attttaagtg ggcaacgcgc aggagcggaa
    28681 catggccctg cgacggtggc aattggttcg ttgattgcag cgttaatttt cgtgccaatt
    28741 ggagcgcttc aggctggtga agcactctgg cactggtcgg ttattccatt gggtctggct
    28801 gtcgctattc tctcgaccgc tctgccttat tcgctggaaa tgattgccct cacccgtttg
    28861 ccaacacgga catttggtac gctgatgagc atggaaccgg cgctggctgc cgtttccggg
    28921 atgattttcc tcggagaaac actgacaccc atacagctgc tggcgctcgg cgctatcatc
    28981 gccgcttcaa tggggtctac gctgacagta cgcaaagaga gcaaaataaa agaattagac
    29041 attaattaaa tttacatttc tgcatggtta tgcataacca tgcagaattt ctcgctactt
    29101 ttcctctaca ccgtctttat atatcgaatt atgcaaaagc atatttattc cgaaaattcc
    29161 tggcgagcag ataaataaga attgttctta tcaatatatc taactcattg aatctttatt
    29221 agttttgttt ttcacgcttg ttaccactat tagtgtgata ggaacagcca gaatagcgga
    29281 acacatagcc ggtgctatac ttagtctcgt taattactgg gacataacat caagaggata
    29341 tgaaattatg agtaccgcta aattagttaa atcaaaagcg accaatctgc tttatacccg
    29401 caacgatgtc tccgacagcg agaaaaaagc aacagtagag ttgctgaatc gccaggttat
    29461 ccagtttatt gatctttctt tgattaccaa acaagcgcac tggaacatgc gcggcgctaa
    29521 cttcattgcc gtacatgaaa tgctggatgg cttccgcacc gcactgatcg atcatctgga
    29581 taccatggca gaacgtgcag tgcagctggg cggtgtagct ctggggacca ctcaagttat
    29641 caacagcaaa accccgctga aaagttaccc gctggacatc cacaacgttc aggatcacct
    29701 gaaagaactg gctgaccgtt acgcaatcgt cgctaatgac gtacgcaaag cgattggcga
    29761 agcgaaagat gacgacaccg cagatatcct gaccgccgcg tctcgcgacc tggataaatt
    29821 cctgtggttt atcgagtcta acatcgaata aatccatcgc tgatggtgca gaactttagt
    29881 acccgataaa agcggcttcc tgacaggagg ccgttttgtt ttgcagccca cctcaacgca
    29941 cttatttagt gcatccatct gctatctcca gctgattaag taaatttttt gtatccacat
    30001 catcacacaa tcgttacata aagattgttt tttcatcagg ttttacgcta aataatcact
    30061 gtgttgagtg cacaatttta gcgcaccaga ttggtgcccc agaatggtgc atcttcaggg
    30121 tattgcccta taaatcgtgc atcacgtttt tgccgcatct cgaaaaatca aggagttgca
    30181 aaactggcac gattttttca tatatgtgaa tgtcacg
//
LOCUS       DAJVRF010000036        29878 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJVRF010000036 DAJVRF010000000
VERSION     DAJVRF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29878
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.036"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            39..581
                     /locus_tag="OQN13_001492"
     CDS             39..581
                     /locus_tag="OQN13_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1614880.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            664..1365
                     /locus_tag="OQN13_001493"
     CDS             664..1365
                     /locus_tag="OQN13_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS1614881.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1390..3989
                     /locus_tag="OQN13_001494"
                     /pseudo
     CDS             1390..3989
                     /locus_tag="OQN13_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3980..5050
                     /locus_tag="OQN13_001495"
     CDS             3980..5050
                     /locus_tag="OQN13_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1614882.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5062..5604
                     /locus_tag="OQN13_001496"
     CDS             5062..5604
                     /locus_tag="OQN13_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1614883.1"
                     /translation="MKKETIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5612..6127
                     /locus_tag="OQN13_001497"
     CDS             5612..6127
                     /locus_tag="OQN13_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1614884.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6093..6830
                     /locus_tag="OQN13_001498"
     CDS             6093..6830
                     /locus_tag="OQN13_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCS1614885.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6941..7951
                     /gene="pyrD"
                     /locus_tag="OQN13_001499"
     CDS             6941..7951
                     /gene="pyrD"
                     /locus_tag="OQN13_001499"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCS1614886.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8125..8667
                     /gene="zapC"
                     /locus_tag="OQN13_001500"
     CDS             8125..8667
                     /gene="zapC"
                     /locus_tag="OQN13_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCS1614887.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8664..9773)
                     /gene="ycbX"
                     /locus_tag="OQN13_001501"
     CDS             complement(8664..9773)
                     /gene="ycbX"
                     /locus_tag="OQN13_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCS1614888.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVMPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10017..12125
                     /gene="rlmKL"
                     /locus_tag="OQN13_001502"
     CDS             10017..12125
                     /gene="rlmKL"
                     /locus_tag="OQN13_001502"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCS1614889.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12137..14044
                     /locus_tag="OQN13_001503"
     CDS             12137..14044
                     /locus_tag="OQN13_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1614890.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQEPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLDQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQMADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14174..15427
                     /gene="pqiA"
                     /locus_tag="OQN13_001504"
     CDS             14174..15427
                     /gene="pqiA"
                     /locus_tag="OQN13_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCS1614891.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSQQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15432..17072
                     /gene="pqiB"
                     /locus_tag="OQN13_001505"
     CDS             15432..17072
                     /gene="pqiB"
                     /locus_tag="OQN13_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCS1614892.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17069..17632
                     /gene="pqiC"
                     /locus_tag="OQN13_001506"
     CDS             17069..17632
                     /gene="pqiC"
                     /locus_tag="OQN13_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCS1614893.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17888..18055
                     /gene="rmf"
                     /locus_tag="OQN13_001507"
     CDS             17888..18055
                     /gene="rmf"
                     /locus_tag="OQN13_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCS1614894.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18125..18643)
                     /gene="fabA"
                     /locus_tag="OQN13_001508"
     CDS             complement(18125..18643)
                     /gene="fabA"
                     /locus_tag="OQN13_001508"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCS1614895.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18712..20472)
                     /locus_tag="OQN13_001509"
     CDS             complement(18712..20472)
                     /locus_tag="OQN13_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCS1614896.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20658..21110
                     /gene="matP"
                     /locus_tag="OQN13_001510"
     CDS             20658..21110
                     /gene="matP"
                     /locus_tag="OQN13_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCS1614897.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21186..22226)
                     /gene="ompA"
                     /locus_tag="OQN13_001511"
     CDS             complement(21186..22226)
                     /gene="ompA"
                     /locus_tag="OQN13_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCS1614898.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22581..23090)
                     /gene="sulA"
                     /locus_tag="OQN13_001512"
     CDS             complement(22581..23090)
                     /gene="sulA"
                     /locus_tag="OQN13_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCS1614899.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23309..23938
                     /gene="sxy"
                     /locus_tag="OQN13_001513"
     CDS             23309..23938
                     /gene="sxy"
                     /locus_tag="OQN13_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCS1614900.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23901..26063)
                     /gene="yccS"
                     /locus_tag="OQN13_001514"
     CDS             complement(23901..26063)
                     /gene="yccS"
                     /locus_tag="OQN13_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCS1614901.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26073..26519)
                     /locus_tag="OQN13_001515"
     CDS             complement(26073..26519)
                     /locus_tag="OQN13_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCS1614902.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26642..28696
                     /gene="helD"
                     /locus_tag="OQN13_001516"
     CDS             26642..28696
                     /gene="helD"
                     /locus_tag="OQN13_001516"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCS1614903.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISSQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28728..29186)
                     /gene="mgsA"
                     /locus_tag="OQN13_001517"
     CDS             complement(28728..29186)
                     /gene="mgsA"
                     /locus_tag="OQN13_001517"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCS1614904.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29282..>29878)
                     /locus_tag="OQN13_001518"
     CDS             complement(29282..>29878)
                     /locus_tag="OQN13_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCS1614905.1"
                     /translation="LRLSTDVDLLVLDGKKVSSSLLRGADSIELDNGPHQLVFRVEKT
                     IHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLENEREANHFDAAPRLELLDGDAT
                     PIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASLPQFATMMADDSTLLSGVSELDA
                     IPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQPSS"
BASE COUNT         7693 a         7059 c         7853 g         7273 t
ORIGIN      
        1 ggcattatca tatttacgaa gagatcagga tgatgacgat gaaaaaaagt gtattgacgg
       61 cgtttataac tgtggtatgt gcaacgtcca gcgttatggc agctgatgat aatgctatca
      121 cggatggcaa agtaacattt aatggtaaag ttattgctcc agcttgtacc ctggtagctg
      181 cgacgaaaga ttcagtagtc acattgccaa atgtcagcgc caccaagttg caaactaatg
      241 gagcagtctc tggcgttaaa actgatgtac caattgcctt agaaggctgt gatgttactg
      301 taactaaaaa tgcgacgttt acctttagtg gtacggctga cggcgtgcaa cctacagcat
      361 ttgccaacca ggctaccacc gatgcagcga cgaatgttgc tctacaaatg tatctgccag
      421 atggttctac atcggtaact cccggtaccg aaacgtcgaa tattcagtta gcggattctg
      481 ctgagcaaac agtgaccttt aaagttgatt atatcgctac ggggaaagcg acttctggta
      541 atgtgaatgc ggtaacaaat ttccatatta actattatta atagaactca ttaattgttt
      601 tattaattag tacccctcta gtgttctgga ggggatattc atatttttta agagtgacta
      661 tttatgaaaa cttgcataac aaagggaatt gtgaccgtaa gtttaacggc aatattactc
      721 agctgctcat ccgcatgggc ggcaggtaaa ggcgggattg gacttgcagc aacacgtctt
      781 gtttattcag aaggtgaaga gcaaatttca ctgggtgtac gtaacaccag tccggatgtt
      841 ccttatctta ttcagtcatg ggtgatgacc ccagataata aaaaatcagc agattttatt
      901 attacaccac cgctatttgt gctgaatccg gctaatgaga atctgttacg cattatgtac
      961 attggtgcgc cgttggcgaa agacagagaa acccttttct tcactagcgt acgggcagtc
     1021 ccttcaacaa cgaagcggga agagggaaat accctgaaga ttgccacaca aagcgtcatc
     1081 aaacttttct ggcgaccaaa aggtttagcg tatcccttag gcgaggctcc ggcgaaactg
     1141 cgttgcactt cgtcagctga catggttacg gtcagtaacc caacacctta tttcattacc
     1201 ctgacagacc tgaaaatagg gggaaaggta gttaaaaatc aaatgatttc cccctttgat
     1261 aaataccaat tctctctgcc aaagggggcc aaaaatagca gcgtaacgta tcgaaccatc
     1321 aatgattacg gggcggaaac gccacaactc aactgtaaat cgtaagccgt cttcagttaa
     1381 gagagcgaga tgtatagaac tcatcgacaa cacagcctgt taagctctgg tggagtgcca
     1441 tcgtttattg gtgggctggt ggtgtttgtg tcggcagcgt tcaatgcaca agctgaaacc
     1501 tggttcgatc ctgctttttt caaagatgat ccctcaatgg tggccgattt gtctcgtttc
     1561 gaaaaaggac aaaaaataac gccaggggtt tatcgtgtcg atattgttct gaatcagaca
     1621 attgtagata cgcgcaacgt caattttgtt gagataacgc cagagaaggg gattgccgcc
     1681 tgtttgacga ctgaaagcct ggatgcaatg ggtgttaata ctgatgcgtt tccggctttt
     1741 aaacaactgg acaaacaagc gtgtgtgcca ttggcggaga ttattccgga tgccagcgta
     1801 acgtttaatg tgaataaact ccgtctggaa atttcagtac cgcaaatcgc catcaaaagt
     1861 aatgcccgtg gttatgtccc ccctgaacgt tgggatgaag ggatcaacgc gctattactg
     1921 ggatattcgt ttagcggggc taacagtatt catagcagcg cagacagtga ttctggcgac
     1981 agctattttc tgaatttaaa cagtggcgtt aatttaggcc catggagatt gcgcaacaat
     2041 tcaacatgga gtcgcagtag tggccaaacc gcagaatgga agaatctcag cagctatttg
     2101 cagcgggcgg ttattccact aaaaggcgaa ctgaccgtag gtgatgatta tactgcaggc
     2161 gattttttcg atagtgtcag ctttcgtggt gtgcagctgg cgtcagatga caacatgctg
     2221 ccagacagcc tgaaagggtt tgcgcctgtg gtgcgtggta tcgccaaaag caatgcccag
     2281 ataacgatta agcaaaatgg ttacaccatt taccaaactt atgtatcgcc tggtgctttt
     2341 gaaattagtg atctctattc cacgtcgtcg agcggtgatt tgttagttga aatcaaagaa
     2401 gcggacggca gcgtcaatag ctacagcgta ccgttttcca gcgtgccatt actccagcgt
     2461 caggggcgaa tcaaatacgc ggtgacactg gcgaaataca gaaccaatag taatgaacag
     2521 caggagagca aatttgccta ggccacgttg cagtggggcg gaccgtgggg aacgacatgg
     2581 tatggtggtg gacaatatgc tgaatattac cgtgccgcca tgtttggtct gggatttaac
     2641 cttggcgatt tcggagcaat ttcgttcgat gcgacccagg cgaagagtac gctggcagac
     2701 caaagcgaac ataaaggtca gtcatatcgt tttctgtatg ccaaaacgct caaccaattg
     2761 ggcactaatt ttcaattgat gggctatcgc tattcgacgt cgggtttcta caccctttcc
     2821 gacaccatgt ataaacatat ggatggctac gaatttaatg acggtgatga tgaagatacg
     2881 ccgatgtggt cgcgttatta caatttgttt tacaccaaac gtggcaaact gcaggtcaat
     2941 atctcccagc aattaggcga gtacggttcg ttttatttaa gtggtagcca gcaaacttac
     3001 tggcataccg atcaacagga tcggctatta cagtttggct acaacacgca aattaaagat
     3061 ctctcgctgg ggggttcctg gaactacagt aagtcccgtg gtcaacctga tgctgatcag
     3121 gtgtttgcac taaatttttc cctgccgctc aatctgttgc tccccagaag taatgatagc
     3181 tataccagga aaaaaattac gcctggatga cctctaacac cagcatcgat aacgaagggc
     3241 acattacaca aaacctgggt ttaacggaga cactactcga tgacggtaac ctgagctaca
     3301 gcgtgcaaca gggatataac agcgagggga aaacggctaa tggtagcgcc agcatggact
     3361 acaaaggggc gtttgcagat gcccgagtgg gctacaacta cagcgataac ggcagtcaac
     3421 aacaactgaa ctacgctctt tcaggcagtt tagttgccca ttcacagggc attaccctgg
     3481 ggcaatcgct gggggaaact aacgtgctga ttgcagcacc aggcgcagag aatactcgtg
     3541 tggcgaacag caccgggctg aaaactgact ggcgcggata taccgttgtc ccctatgcca
     3601 cttcttatcg ggaaaatcga atcgcacttg atgcggcatc gttaaaacgt aacgtggatc
     3661 ttgaaaatgc agtagtcaac gtggttccca ccaaaggggc gttggttctg gcggagttca
     3721 atgcccatgc gggagcaagg gtattaatga aaacatcaaa gcagggtata ccgctgcgtt
     3781 ttggcgcgat agcgacgctg gacggcgtac agactaatag cggcattatt gatgatgatg
     3841 gctcgctcta tatgtccggt ttgcccgctc agggagcgat aactgtacgc tggggcgaag
     3901 ctcccgatca aatttgtcat atcagttacc agcttaccga acaacaaatt aactctgcga
     3961 ttacgcgaat ggatgccata tgcagataat ctttggagaa aaatgcgtgt tattactacg
     4021 actatttttt gccgccgtct taatgctatg gtgcgctcaa actgctgctt atagcgggca
     4081 gtgtcatacc actcagggga atccgtatat tggcgtcaat tttggcgtta aaaccctgga
     4141 ggaagaagaa aatacggctg gagtagttaa agacaaattt tatcagtgga acgaatcgaa
     4201 tgattattat gtttcctgtg attgcgataa agacaatgtc agaagtggcc gatgggcatt
     4261 cgccgcggat tcaccgttag tctatttagg cgacaactgg tacaaaatta atgactatct
     4321 tgccgccaaa gttttattgc aggttaaagg cagttctcct actgcggttc ctttcgaaaa
     4381 cgtgggcaca ggggcagata cacgatggca tatttgcgat cccggcggtc aacgtttagg
     4441 tggccagggg gctagcggta atagcggtag cttttccctg aaaatattgc agccgttcgt
     4501 tggttcggtc gtcattcctc ctatggcgct ggcgcgatta tttgaatgct acaacatacc
     4561 cgcaggtgat tcctgcacga ctacaggtac accggtttta gtgtattacc tgtctggtac
     4621 gatcaattca cttggctcat gttccgtcaa tgccggagaa acaatcgagg tcgatctggg
     4681 cgacgcattt gcggctaact ttcgtgttgt agggcataag cctcttgggg ccagaacggc
     4741 agaacttgca attccagtca ggtgtaacac gggaaacgcg gggttagtta acgtcaacct
     4801 gagtctgacg gcaaccacag accccagcta tccccaggcg attaagacgt cacgtcctgg
     4861 cgtgggcgtg gtggtgaccg atagccagaa caacattatt tcccctgctg gtggaacatt
     4921 accgctctct attcctgatg atgcagacag tatcgcgcga atgaatgtct atccagtcag
     4981 cacgacaggt gtaccaccag aaaccgggcg atttgaagcc acggcaacgg tgagaataaa
     5041 ttttgattaa cgcgtgaacg tatgaagaaa gaaacgatat ttcagtgcgt tatcttgttc
     5101 tttagcattc ttaacatcca tgtcgggatg gctgggcctg aacaagttag tatgcatatt
     5161 tatgggaatg tggtcgatca gggctgtgat gtcgccacca aaagtgcatt acaaaatatt
     5221 catattggtg attttaatat cagtgatttt caggccgcga ataccgtaag cactgctgct
     5281 gatttgaata tcgatatcac cggttgtgcc gctagtatta ctggcgcgga cgtccttttt
     5341 agcggcgagg ctgacaccct tacgccgaca ctgctcaaac taactgacac aggcggaagc
     5401 ggtggtatgg caacggggat tgccgtgcaa attcttgatg cgcaaagtca gcaagaaatc
     5461 ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac actcaaatat
     5521 caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac ggcggttctc
     5581 tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct ggattctgtt
     5641 cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg tcaacattag
     5701 cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa tcaatgtgca
     5761 gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg agaaatcttt
     5821 catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgacgtca ccttttcagg
     5881 aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg atgcagccag
     5941 cgggctggca attgcgattc tggatgatgc gaagatattg attccgctta accaggcgag
     6001 taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc aattgcgacc
     6061 tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg tacttcatta
     6121 tgactaatac gtggaacaga ttggcgctct tgattttcgc cgttttatcg ctgctggtgg
     6181 cgggtgaatt gcgggcagga gtggtggtcg gcggaacgcg atttatcttt ccggcagaca
     6241 gagaatcgat atctatttta ctgactaata ccagtcagga atcctggctt attaacagta
     6301 aaatcaaccg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca gcaccattac
     6361 tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg cgcttgctga
     6421 gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta agcattgcca
     6481 gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc tcggtattta
     6541 aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat caacaattac
     6601 gctggacacg gaattcgcag ggtgtacaac tcactaaccc aacgccttac tacattaacc
     6661 tgattcaggt gagcgtaaat ggtaaagcgt taagtaatgc gggagttgtg ccgcctaaaa
     6721 gccagcgtca gacaagctgg tgtcaggcaa tcgcaccgtg tcatgtcgcc tggcgggcga
     6781 taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga aacaggttcg
     6841 gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa ctcgcacata
     6901 atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc ccttcgttcg
     6961 taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc agcaattacg
     7021 ccgtattaca ggaacgccgt ttgaagcact ggtgcggcag aaagtgcctg cgaaacctgt
     7081 taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc ttgataaaga
     7141 cggggagtgc attgacgcgt taggcgcgat gggatttgga tcgatcgaga tcggtaccgt
     7201 cacgccacgt ccacagccag gtaatgacaa gccgcgtctc tttcgtctgg tagatgccga
     7261 aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg tagagaacgt
     7321 aaaaaaggcc cattatgacg gcgtcctggg tattaacatc ggcaaaaata aagatacgcc
     7381 agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg cctatgcggg
     7441 atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc tgcaatatgg
     7501 tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt tgcaagcgat
     7561 gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg aagaagaatt
     7621 gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg caaccaatac
     7681 cacactcgat cgttctcttg ttcagggaat gaaaaattgc gatcaaaccg gtggcttaag
     7741 tggtcgtccg cttcagttaa aaagcaccga aattattcgc cgcttgtcac tggaattaaa
     7801 cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg cgcgtgaaaa
     7861 gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta aaggtccgcc
     7921 gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc tttataagat
     7981 aatcagggct ttattttcag ccctggttgt tttatattca tcgctgttgc ttatttagac
     8041 attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt gttgctgcgg
     8101 ttttataggg taggggagag gcagatgcga attaaaccag acgataactg gcgttggtat
     8161 tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct atttcgctca
     8221 cgttttgcgc gcaagatgtt gactccagat gccttttctc ccgcaggctt ttgcgttgac
     8281 gacgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt atctaaagaa
     8341 caaaaagccg aactggtgct gaatgcactg gtagcaattc gctatctcaa accgcaaatg
     8401 ccgaaaagct ggcattttgt ttcccatggt gaaatgtggg tgcccatgcc gggcgatgcg
     8461 gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt tgaatctggc
     8521 gaaaatgccg cactatgcct gctggcacaa ccctgcgttg ttattgcggg tcgcgcgatg
     8581 cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt taatgttgac
     8641 agcttcagcc tcgaacaggc agtctaacgc gccaacttaa gtgcagtctt cggtacacag
     8701 ctacagcaaa gaatggtgcc atcatcgccc attgctgatt ttttcagcgg catgacttcg
     8761 ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga ataagggata
     8821 cgaattccct gattttctaa ttgctccagc aacacctgtt ggttatttcc acgaaatgcc
     8881 tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt ggcggtatca
     8941 tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc cacctcatcg
     9001 ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc gacatcgccg
     9061 ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc cggatgtttt
     9121 tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt aaccacatca
     9181 aacaccacat caccaatgcg aatcactttc cagcgatctt cttcccaggc tgacgcgccg
     9241 gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca acgttgttgg
     9301 agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc aaatgacaga
     9361 ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca gcgtaattgc
     9421 acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc aattcgcgcg
     9481 gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc agcaaaacga
     9541 acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgcac aggtgaagga
     9601 gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc aggttccgtg
     9661 atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg tgtaagacca
     9721 atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcgc cacggtgcgg
     9781 tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag ctataatgcg
     9841 caacaatttt cttagcgcct gtcccattag gctattttat ttgtcagttt ggccctgggt
     9901 tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctg tccatgtcca
     9961 gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt gacgatatga
    10021 attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact gaactggaaa
    10081 acctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag ggcgacacac
    10141 ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg ttgccgctgg
    10201 gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg atcaactgga
    10261 cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg aatgacacca
    10321 tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat gctttcacgc
    10381 ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc gttaacgtct
    10441 ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt ttacatctgc
    10501 gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca gccgctattg
    10561 tgatgcgaac cggctggcag ccaggaacac cgctgctcga tccgatgtgt ggttccggta
    10621 cgttgctgat tgaagcggcg atgctagcga ccgatcgcgc accaggcttg caccgtgggc
    10681 gttggggctt tagcggctgg gtgcagcatg atgaagctat ctggcaggaa gtgaaagcgg
    10741 aagcgcaaac tcgtgcccgt aaaggcctgg ctgagtatag ctctcatttt tacggttcgg
    10801 acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg gggattggtg
    10861 aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg ccgaaagggc
    10921 cgtacggcac agtgttgagc aacccgccat acggggaacg tctggacagc gaaccggcgc
    10981 tgattgcgct gcatagcctg ctgggtcgga tcatgaaaaa ccagtttggt ggctggaatc
    11041 tctctttgtt tagtgcctcg ccggatctgc taagctgctt gcagctgcgt gcagacaaac
    11101 agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac gttgccgaaa
    11161 gcactccaga cagcaaaccg gcgatggtag cggaagacta cgccaaccgc ctgcgtaaga
    11221 acctcaaaaa attcgagaag tgggctcgcc aggaaggaat tgaatgttac cgcctgtatg
    11281 acgccgatct accggaatat aacgttgccg ttgaccgtta tgccgactgg gtggtggtgc
    11341 aggagtatgc gccgccaaaa actattgatg ctcacaaagc gcgtcagcgt ctgttcgata
    11401 ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg ctgaaaaccc
    11461 gtgaacgcca gaagggcaaa aaccagtacc agaaactggg cgagaagggc gagtttcttg
    11521 aagttaccga atataacgct cacttgtggg tgaacctgac ggattatctc gatactggtc
    11581 tgttcctcga tcaccgtatc gcccgccgta tgctcggtca gatgagcaaa ggcaaagatt
    11641 tcctcaacct gttctcttat accggtagcg ccaccgtgca tgcgggatta ggcggcgcgc
    11701 gcagcaccac caccgtggat atgtcgcgta cttatctgga gtgggcagaa cgcaacctgc
    11761 gtctgaatgg cctgaccggg cgtgcgcatc gcctgattca ggccgattgc ctggcgtggc
    11821 tgcgtgaggc aaatgaacag tttgatctga tctttatcga tccgccaacc ttctctaact
    11881 caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tatggcgctg atgaaagatt
    11941 tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa cgtggcttcc
    12001 gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt acgcaaaaaa
    12061 cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg attaccgcag
    12121 cctgaaagga atagtaatgt cattaatcag tatgcatggc gcatggctgt cgttcagcga
    12181 cgcgccgctt ctcgataacg cagaactgca tatcgaagat aacgaacgtg tttgtctggt
    12241 gggtcgcaac ggcgcgggca aatcgacgtt aatgaaaatc ctcaaccgtg aacaagggct
    12301 ggatgacggt cgcattattt acgagcaaga tttgattgta gctcgtctgc aacaggagcc
    12361 gccgcgtaac gttgagggta gcgtttatga tttcgttgcc gaaggcattg aagaacaagc
    12421 ggaatatctg aaacgctatc acgatatttc gcgcctggtg atgaacgacc cgagcgagaa
    12481 aaatctcaac gaactggcga aggttcagga acagttggat caccacaacc tgtggcagct
    12541 ggaaaaccgc atcaacgaag tgctggcgca actggggtta gatcctaatg ttgcgctgtc
    12601 gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg tgagtaatcc
    12661 gcgcgtgctg ttgcttgatg aaccgacaaa ccacctggat attgaaacca tcgactggct
    12721 ggaagggttc cttaaaacct tcaatgggac gattattttc atctcccacg accgttcgtt
    12781 tatccgcaat atggcgacgc gcattgttga tctcgatcgc ggcaagctgg tgacctatcc
    12841 agggaattac gaccagtacc tgcttgaaaa agaagaagcc ctgcgcgtgg aagaactaca
    12901 aaatgccgag ttcgatcgca aactggcgca ggaagaggtg tggatccgcc aggggatcaa
    12961 agcacgccgt acccgtaatg aaggccgcgt acgcgccctg aaagcaatgc gtcgcgagcg
    13021 tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag gtggaagagg ccagccgctc
    13081 cggtaagatc gttttcgaaa tggaagacgt ttgctaccag gttgacggta agcaactggt
    13141 gaaagatttt tcggcccagg ttctacgtgg cgacaaaatt gccctgattg gtccgaatgg
    13201 gtgcggcaaa accacgctgc tgaaactgat gctcgatcag cttcaagcgg acagcgggcg
    13261 tattcacgtt ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg cggaactgga
    13321 tcccgataaa acggtgatgg ataaccttgc cgaaggtaag caagaggtga tggttaacgg
    13381 caagccacgc cacgtattgg gctatttgca ggactttctg ttccatccga aacgggcgat
    13441 gacgccggta cgtgcgcttt ctggcggtga gcggaaccgc ttgctgctgg cgcgtttgtt
    13501 cctcaaacca agcaacttat tgattcttga cgaaccgacc aacgatcttg atgtcgaaac
    13561 gctggaactg ctggaagaac tgatcgacag ctatcagggc acggtattgc tggttagcca
    13621 cgatcgtcag tttgtcgata acaccgttac agaatgttgg atcttcgaag gcggcggtaa
    13681 aattggtcgt tatgtcggcg gttatcatga tgcccgtggt cagcaagagc agtatgtggc
    13741 gctcaaacag cctgcggtga aaaaaaccga agaagccgcc gcggcaaaag cagaaactgt
    13801 aaaacgcagc agtagcaaac taagctataa attgcagcgc gaactggagc agctaccgca
    13861 attgctcgaa gatctggagg cgaagctgga agccctacag acgcagatgg cggatgcttc
    13921 cttcttcagt cagccacatg agcagacaca aaaagtgctc gccgatatgg ctgctgcaga
    13981 gcaggagctg gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa aaaatggtgg
    14041 ctgatcgcaa aatagtcgat ttaaacagag acagctgaca acggtaaaat tgcctgatga
    14101 actgggttta tcaggcatga aaaccaacac tttgttagcc atctgtaaaa tagcgcatca
    14161 ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt gctcgcagtg
    14221 tgacatgctg gtggcgttac cgcgccttga gcatggtcag aaagcggcat gtccccggtg
    14281 tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag cgtccgaccg cctatgcgtt
    14341 ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata tgaacgttgc
    14401 aggagttacc agtgaaatta cattactgga aattcccggc gtgctttttt ctgaggacta
    14461 cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt tttgtctgat
    14521 aaccattctg ttactggtga atcgcgcgga attaccggtc cgtttaaaag agcaactggc
    14581 acgggtgctt tttcaactca aaacctgggg aatggcggag attttcctcg ctggtgtgct
    14641 ggtcagtttc gttaaactga tggcttacgg cagcattggg gtaggcagca gctttctccc
    14701 ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc gtcgctggtt
    14761 atgggacgac atcgccccga tgccagaact gcgccagccg ctaaaaccag gcgtcacggg
    14821 gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca atccttcctg ctgatgaacc
    14881 cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt agaaacagcc agcagtggac
    14941 actcgcgctg cttgtaacgt ccatcatgct gtatcttccg gctaatattt tgcccatcat
    15001 ggtgacggat ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg tcattctgtt
    15061 atggagcgaa ggctcttatc ccgtcgctgc ggtgatcttt ctggccagta ttatggtgcc
    15121 aacgttaaag atgatcgcca tcgcgtggct gtgttgggat gccaaagggc atggcaagcg
    15181 cgacagtgaa agaatgcatt tgatttatga agttgttgag tttgtaggcc gctggtcgat
    15241 gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag gtttaatgag
    15301 tatttatccg gcaatgggtg cattaatgtt tgctttagtc gtcataatga caatgttttc
    15361 tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag agcatgagga
    15421 gtcctgacag tatggaatct aataatgggg aagccaaaat ccagaaagtg aagaactggt
    15481 ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg ggcctgggtt cttttttatc
    15541 attacagcca tcagggactg gaagtgaccc tgatcaccgc gaatgcggaa ggaattgaag
    15601 gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa agcgccacac
    15661 tggctgatga tttgacgcac gttgaaatca aagtgcggct gaattctggt atggaaaaat
    15721 tgctgcataa agacaccgtc ttttgggtgg tgaaaccgca gattggtcgc gaagggatta
    15781 gcggcctggg aacgctgctg tctggagttt atatcgaact gcagccaggc gcgaaaggca
    15841 gcaaaatgga taaatacgat ttgctggact cgccaccgtt ggccccgcct gatgcgaaag
    15901 gtatccgtgt gattctcgat agcaaaaaag ccgggcagct ctcgccagga gatccggtgc
    15961 tgttccgtgg ctatcgggta ggttcggttg aaaccagcac cttcgatacg caaaaacgca
    16021 atatcagtta tcaactgttc atcaatgcac cttatgaccg actggtgacc agcaatgttc
    16081 gcttctggaa agatagtggc attgcggttg atctgacgtc agcggggatg cgtgtggaga
    16141 tgggctcatt gacaacgctg cttagtggcg gtgttagctt tgatgtgccg gaaggtctgg
    16201 atttagggca gccagtggca ccgaaaacag ctttcgtttt gtatgatgat cagaagagca
    16261 ttcaggattc gttgtacacc gatcacattg attatctgat gttctttaaa gattcggtac
    16321 gcggtctgca accgggagct ccggtagaat tccggggtat tcgcctgggt accgtaagca
    16381 aagtgccatt ctttgcgccg aatatgcgtc agacatttaa cgatgattac cgtattccgg
    16441 tactgattcg tatcgagcca gagcggctga aaatgcagct tggcgaaaat gcggatgttg
    16501 ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa accggaaacc
    16561 tggtcactgg tgcactgtat gttgatcttg atttctatcc aaatacgcct gcaataaccg
    16621 gtattcgtga atttaatggt tatcagatta tcccgactgt tagcggcggc ctggcgcaaa
    16681 tccagcaacg actgatggaa gcgttggata agatcaacaa actgccattg aatccgatga
    16741 ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac ctgcaaacga
    16801 cgctggatag catgaacaag atcctcgcca gccagtcgat gcagcagttg ccgacggata
    16861 tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct ggctccgcag
    16921 cctacaacaa gatggtggcg gatatgcagc gccttgatca ggtgttgcga gaactgcaac
    16981 cggtgctgaa aacgctcaat gagaagagta acgcgctggt atttgaagcg aaggacaaaa
    17041 aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga ttgcagcact
    17101 gtggctggcc ggatgcagct ccggcgaaat taataaaaac tattaccagt tacctgtggt
    17161 gcagagcggt acacaaagta ccgccagcca gggcaatcgt ctgttatggg tagagcaggt
    17221 cactgttcct gactatctgg cggggaatgg tgtggtttat caaaccagtg atgtgaagta
    17281 tgtgattgcc aacaacaact tgtgggccag cccgttggat caacaattgc gcaacaccct
    17341 ggttgccaac ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc ctctgggaag
    17401 cgcccaggac acgctcaatg ttaccgtaac ggagtttaac ggtcgctatg atggcaaggt
    17461 cattgtcagt ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac gtccgttccg
    17521 tctggaagga gtgcaaactc aggatggtta cgatgagatg gttaaagtac tggccagtgt
    17581 ctggagtcag gaagccgctt ctattgcgca agagataaag cgtctacctt aattataaag
    17641 atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt ttctaacgtg
    17701 cggaaaaagt tgttcctctt tacatttttt gtacaaccga catacccgtg tagctcacaa
    17761 atatgacagt ggcgtgaatt ttgcgcattg acggcagtta tgattcgcgg tattgcttaa
    17821 ctgtgattgc acatttagta atcactgttt tcttttccac cagaaaccag tatgagggaa
    17881 acgaggcatg aagagacaaa aacgagatcg cctggaacgg gcacatcaac gtggttatca
    17941 ggccggcatc gccggacgct caaaagaaat gtgtccctat cagacgctga atcaaaggtc
    18001 acaatggctg ggaggctggc gagaagccat ggcggacagg gtagtaatgg cctgattctg
    18061 tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc ttttgatact ctgtctgatt
    18121 ataatcagaa ggcagaagta tcctggaaca gaccgacttt caggtcgctg gcggtataga
    18181 tcaaacgacc atcaaccagc acttctccat ccgccaggcc cataatcaga cgacggttaa
    18241 caatgcgttt aaagtgaata cggtaggtca cttttttcgc tgtcggcagt acctgaccag
    18301 tgaatttcac ttcgccaacg cccagtgcgc ggcctttacc ttcgccgccc agccagccga
    18361 ggtagaaccc taccagctgc cacattgcgt ccaggcccag gcatcccggc ataaccggat
    18421 cgccaataaa gtggcatccg aagaaccaca gatccggatt gatatccagt tctgcttcaa
    18481 catacccttt gtcgaagtta ccacccgttt cggtcatttt gaccacacgg tccatcatca
    18541 gcatattcgg tgctggcaat tgcgggcctt tagcgccaaa cagttcaccg cgaccagagg
    18601 caagaaggtc ttcttttgta taggattcgc gtttatctac catgttctct gtaagcctta
    18661 ttttattgaa gcacgcagga tagctaacac gtgtacgctg aacaagtccg atcagttcgg
    18721 aataaaccag ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt cctgttgcga
    18781 tgcttgcgcg atacgttcct ggatggtttg catcagcgtc gtttggcctt cgccatccca
    18841 caccagattt aataataacg gcagtgcgtc agtcacatcg tctactgccc agatggtgaa
    18901 tttgccttct tctaccgctt tcactagttc actgtgaaga cttaaatggc gaacgttagc
    18961 tgtggggata atgacacctt gtttcccggt taactcacgt tgctggcaaa tagcaaagaa
    19021 gccttcgatt ttctcattta aaccaccgac cggctgggcg cgaccgaact gatcgactga
    19081 acctgtgata gcgatactct gattcaccgg cacatcggcg agggcgctta tcagggcgca
    19141 gagttcagcc atcgaggcac tatctccatc aacttcactg tatgactgct caaatgtcag
    19201 cgatgctgag aaggggatct gttgctcaag ctgtagttcc gacatcagga acgcttgcat
    19261 gatcatcatc cctttcgcat ggatattgcc gccaagctcc gctttgcgtt cgatgtcggt
    19321 gaattcacca tcgccaatat gcacaacgca gctaatgcga gaaggttcgc caaaagcgcg
    19381 tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc gttcgccttc
    19441 ggtttcaatc aggatttgct cctgaaggat ctcatcctgc atacgttcag cgaggaaacc
    19501 ttcgcgccat tcacgctgct gcagcattaa gtttagctgc tcgccggaga aggtgtcgcc
    19561 atcacacagg gaggcgacct ctttacactg gcggaggatc cactgcgggc taagcggaag
    19621 tgtttcttgt tcaccggtgt agcgtgctgc ttcgcggata agtaccggcc aggcatccgc
    19681 tcccggtgca ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac accactgggt
    19741 tactgactcc gcatcgacaa tctgcagagt atcttcaaat tcgctataaa tagcctgctc
    19801 tgaaagctct ggctccatct cctggaaatc agccaatgat tcgcgttcgc ctaccagaat
    19861 gactttcagc ttcaatggca tcgaaggcac agagacgggg agagggcgcg actcatcaaa
    19921 cgcaacccag tcaaaacgct cgcggttaac gatatttttc agccgcatcc acagcagagg
    19981 ttgcgccagc agtgtacgca aagagatgat gagaataccg ccatttgcct gatgcaccag
    20041 accaggctgc agggtaatgt cgccattaaa ctggcgcagg cagccaaaga gttgctccgc
    20101 ttctacccag tcggcagcga caacttgcgt taaagtcgca aaattatcat ctgcactcac
    20161 tgcgtgacgt aagcggatgg agtggccgga aacctcatag tgaccgccca ccagttggcc
    20221 tgcatcgctt tgtagcgtac gcgcggcatt ggcaataaga ttcagatact cagactcttc
    20281 cggggccttc gccagcataa aagaggagga tgctcgcgta tgcagcaact gctccagcgc
    20341 aaattgcagc cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa tcaaatgtgg
    20401 ctgagcaaat atttcctgat agctatcggt atcaggaacc aggtcacgcc atgcaagttt
    20461 cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta taccgtaacc
    20521 ggcactacag cacacgtaga aagcaccgac aatactccag gcatgggcgt taaagctcac
    20581 aggatggaga ttcttttctt cactggccta aaaagctgat attctgtaaa gagttacacg
    20641 gtaacattga gatcgctatg aaatatcaac aacttgaaaa tcttgaaagc ggttggaaat
    20701 ggaagtatct ggtgaagaag catcgcgaag gggagttaat cacccgttac atagaagcca
    20761 gtgccgccca ggaagccgtc gatgtgttgc tctcgctgga aaatgaaccc gtgctggtaa
    20821 atggctggat tgacaagcat atgaatccgg aactggtcaa tcgcatgaag caaaccattc
    20881 gggcaagacg aaagcgccat tttaatgcgg aacaccagca tacgcgcaaa aaatcgatcg
    20941 atctggaatt tattgtctgg caacgtctgg ctggtcttgc gcagcgtcgc ggtaaaacgc
    21001 tttctgaaac gattgttcag ctgattgaag atgcggaaaa caaagagaaa tacgcgaata
    21061 aaatgtcttc tctgaagcag gatctgcagg cattgctggg taaggaataa ctgacgaaag
    21121 tcagttcaat ttactaaagg caaaaaaaac cccgcagcag cggggttttt ctaccagacg
    21181 agaacttaag cctgcggctg agttacaacg tctttgatac ctttaacttc gatctctacg
    21241 cgacgatccg gagccaggca gtcgatcagt gcagcacgct gtttcacgtt gtcacaggtg
    21301 ttgccagtaa ccgggttgga ttcgcccata ccacgtgcgg agatcttgtc tgccgggata
    21361 cctttggaga tcaggtaatc aacaacagac tgagcacggc gctcggacag accctggttg
    21421 taagcgtcag aaccgatgcg gtcggtgtaa cccagaacaa ctacggaacc gtctttcgga
    21481 tccaggttgc tcagctggct gtacagctga tccagagcag cctgaccttc cggtttcagg
    21541 gttgctttgt tgaagttgaa cagaacgtca gacttcagag tgaagtgctt ggtctgtact
    21601 tccggtgccg gagctggagc cggagcaact actggagctg cttcgccctg accgaaacgg
    21661 taggaaaccc ccaggctcag catgccgttg tccggacgag tgccgatggt gtgtgcgtca
    21721 ccgatgttgt tggtccactg gtattccaga cgggtagcga tttcaggagt gatcgcgtac
    21781 tcaacaccgc cagcgaagac cggagaaacg ccggtgtcgt ggtttttacc ataaacgttg
    21841 gatttagtgt ctgcacgcca taccatacca cccagacgag tgtagatgtc caggtcgtca
    21901 gtgattgggt aacccagttt agcggtcagt tgaacgccct gagctttgta tgcaccgttt
    21961 tcaacgctgc ctttgtacgg catacgacct aaccagtcgt aacccatttc aaagccaaca
    22021 tacgggttaa cctggtaacc accaaaagca ccagcgccca gttggttttc atgggtcggg
    22081 ccattgttgt tgatgaaacc agtgtcatgg tactgggacc agcccagttt agcaccagtg
    22141 taccaggtgt tatctttcgg agcggcctgc gctacggtag cgaaaccagc cagtgccact
    22201 gcaatcgcga tagctgtctt tttcattttt tgcgcctcgt tatcatccaa aatacgccat
    22261 gaatatctcc aacgagataa cacggttaaa tccttcaccg ggggatctgc tcaattaact
    22321 ctaccgatat cttcggctta tgccgagcac ccctggcgat gtaaagtcta caacgtagtt
    22381 gaaaacttac aagtgtgaac tccgtcaggc atatgaaaaa aaagtcttgt ataaggtatg
    22441 tttaatcttt tttgtcagcg acaatttaca gaagaaaatc gcggaaaccg cttcagacaa
    22501 gcctccgcaa ggaaaattag tcacgactga aagcattggc tgggcgacaa aaaaagttcc
    22561 aggattaatc ctaaatttac ttaatgatac aaattagagt gaatttttag cccggaaagt
    22621 tgtctcgtgg cgtgagagga tgcgcttacc ggacgcataa taaaccccat agcgttacct
    22681 tcatttgccg catcaacaag ttcagcatgc tcttcttcag tcaaatcatc tgccaaccaa
    22741 ccgatcacca cactgtaatt gcccgtgcgt aaagcgcgaa ccattgactc cacagtgtgg
    22801 caaggggaga gctggctaat ctgcattact ttcgttaagg gtagcccaga tgcctgaacc
    22861 cattcccgac tcagtttttg ttgcggtgtt aaccagagtt gccagcgcga ttgctgaccg
    22921 agttgctgta acaatggcaa cagtagaagt tgcgtcatca tgggctgatc ttcgcgatag
    22981 acaacttcac tgataagccc ggctgtagtg ttttccgtag agacacgcgc aattttactt
    23041 gctgcggatg agaacgacga agaacgatgt gcatagcctg aagtgtacat aatcaatcca
    23101 gcccctatga gttactgtat ggatgtacag tacatccagt gacaacaaag atcaacccta
    23161 ttttcggaaa gagcctcgca aattttgtcg ttggtgacgg gaaaacataa attaatcttg
    23221 ccccttaaga ataagttgcc tattttcgta gttaacggat ccgttaatgt gaatcattct
    23281 tttatgttat gattttaaaa ggaattttat gaaaagcctc tcctataagc ggatctataa
    23341 atcacaagaa tacctggcaa cgttgggcac aattgaatac cgatcattgt ttggcagtta
    23401 cagcctgacc gttgacgaca cggtgtttgc gatggtttct gatggtgagt tgtatcttcg
    23461 ggcttgtgag caaagtgcac agtactgtgt aaaacatccg cctgtctggc tgacatataa
    23521 aaagtgtggc cgatccgtta ccctcaatta ctatcgggtt gatgaaagtc tatggcgaaa
    23581 tcaactgaag ctggtgcgtc tgtcgaaata ttctctcgat gcagcgctga aagagaaaag
    23641 cacgcgcaat acccgggaaa gactgaaaga tttgcccaat atgtcttttc atctggaagc
    23701 gattctcggg gaggtgggga ttaaggatgt acgggcgtta cgtatacttg gggcaaaaat
    23761 gtgttggttg cgactgcggc agcaaaacag tctggtgaca gaaaagattc tgtttatgct
    23821 tgaaggtgcc attatcggca ttcatgaagc tgcgctcccg gtggcacgcc gccaggagct
    23881 tgcagaatgg gctgactctc ttacgccgaa acaggagttt cctgcggaac ttgagtaatc
    23941 tggcgttgca gacgaccaat ctcaggcagt aatgcaatca ataatccaac ttgttgtacg
    24001 accagaggtt ctttgctgtc tgcccgtggt tcaagttgct gcatccgctg tttcaggcta
    24061 gctaatgcct cattgacgcg ttcttcatca gcaggttgat gatgtaacgc gtcatcaaca
    24121 tagcaaactg cgtcatcaag aaacgccaga atttcaggat tagttaactg ctcccggtga
    24181 gcaccgaggg ctgagatata gctggtaaac gtatggttaa ggcacagcaa ccgaaacgcg
    24241 gcttcgcgaa tttgcggggt aacgttcggc tcgctggaca tatttgatac caccgacgcc
    24301 agctcagcat cacggttgtg tgcatcgcgg cgggcaatac gatacgccag acggttatca
    24361 cgcccctgat ggtattgctc cagtatggca tcgagatacc gacagttggc ctctgtggcg
    24421 cgttcgagca tgcgcggcag attgcgaaac tgccagtcag gccagatgta gctcactgcc
    24481 gcccacgcaa tggcacaacc aatcagcgta tcgattacgc gaggtaacgc tacttcaaaa
    24541 ccttcaccca gtaagttaaa acacagtagc accaaaagtg tgatgaacat cgttgcatga
    24601 gcgtattgca cgttacggaa ggcaaaaaag agcacgccgg taataaccag cagcaccagc
    24661 tgcccttcca gtgatggcac aaaccacagc acaggaatgc caatggcgat acctaccagc
    24721 gtaccaataa tccttaactt caggcggtgg cgcgtggcgt tatagtttgg ctggcagaca
    24781 aacaaacttg tcagcaagat ccaatacccg tgatgcattc cggttatctg aatgatggcg
    24841 tagccgaagc acaataccag cgacattctt accgcatgac ggaagagggc ggattccggc
    24901 gtgaagtgac ggctaagacg cagccagata tcactcaacc cgtgcgggct gtcatcagcg
    24961 agctcatttt cgtcattatt atggggtagt gcctgggcct gttctgattc aattgttgcc
    25021 agttgggcat caatggcgcg taaattgttc agcaaaaatc ccagtgtttt gagtaaatcg
    25081 gcgggtgcgc cgttatcgcg catccgctcc agcgcagcat caatatgcgt aaaagcgcgc
    25141 tcaaaatgcg gatcatgttg ataaggctga cgcaacaaaa tacagcgtga cagttgctgg
    25201 cacgcctggc cctgcatcga catcagccgc tgaaaacgga acagcacgtc gctgtggcga
    25261 aaatgttcac gcaatgtttg atactgaata tgagaagagc tggcacgctc gtgaatatcc
    25321 tgtgcgacaa agtaataatg cagcgtgcga cgcgttcccc gttgaccacg atcgccacgt
    25381 aagcgggtca gcagcgagag tttcgtctga ttcaatgtcg ccatcagcag accgttggcg
    25441 agagccaaat cgtacagcgg tgcctggctt tcatcttcaa tatcaggatc aaacatgcgc
    25501 gacttgagct caagataacg cgccagttgt tcatagcaac gcgccaggtt gtcctgcagc
    25561 gggcggaccg ggaacagcag atgaccaata agtgtcagga cgttgtacca gacggcaccg
    25621 gccagcagat acatcggctg ctgataccag tgctcataca gtgatgttcc caacatagtg
    25681 taaatggcga tcagcaatgc accgaaggca attgttgcat agcgttgacc cagaccgccg
    25741 agcaaaatga agccgctggt agagagcgtt aagccaatcg caaatagcca gggccaggga
    25801 aacagcaatt ctactgaggc cgaggcgata aaaaagcaga acagcgtaat gatgaggtta
    25861 cgcaaacgtc ccgccagtcg gtcatcgaga tcggtcagcg ccgctgccac catccccagc
    25921 gttagcggaa tcgtcagttt tacatcaccc agccaccacg gaaacgctgt ggttccacaa
    25981 agcgcaataa aaatacgcgc gtaatacagc caggcgctgt tccaggtata gcgtttgagc
    26041 aaaggactta gcataaaggc catattagtg gattattcaa aacgacgacg tgcattggct
    26101 tcacgcgcag cttgcgctgt ttctaccgat accacgcgac gaccaaccgg ccatagtgca
    26161 atagcggcaa ttttaaagtt cgcaatgccg acaggaatgc caatgattga aatacattgt
    26221 gcgatgcccg ttgcaatgtg catcaggcat aaccaccagc caaagaaaat cagccagaaa
    26281 atattcaata ccgtaccgcc agtattcagc agcacatttt tgccagccgg gttcagttca
    26341 tcgacatgaa tagcttcatt gccataaggc accagagata gtttagtgat ctcccagcag
    26401 gatcgtgtca gcggtaaggt aaaaatcagc acaatactga ccagagtcgc caacagccag
    26461 cccagagtgg tggcaaatcc gccaagcaca aagttcagaa tgttcaaaac ggtacgcata
    26521 aaacctcgct ttactgtggt tttcagtaat gggcggcaat tgtaacgttt ttttgggctg
    26581 gagcacgttt tctctgacgg ttacactgat aagaaataat ttcgtgtgga tctacagagt
    26641 catggaactg aaagcgacaa cgcttggaaa acgtctggca cagcaccctt acgatcgggc
    26701 ggtgatcctc aatgccggga ttaaagtctc cggcgatcgc cacgaatacc ttattccttt
    26761 caatcaatta ctggcgattc actgtaagcg cggtctggta tggggcgagc tggaatttgt
    26821 actgccggac gaaaaagtgg tgcgtctgca cggcaccgaa tggggcgaga cgcagcgttt
    26881 ttaccatcat cttgatgctc actggcggcg gtggagtggc gagatgagcg aaattgcgtc
    26941 tggtgtttta cgccagcaac tggatttgat tgccacgcgc actggagaaa ataaatggct
    27001 gacgcgtgag caaacctctg gcgttcagca acaaatccgc caggctttgt cggcgttgcc
    27061 gttgccggtt aaccgactgg aagaattcga taactgccgt gaggcgtggc gtaaatgtca
    27121 ggcctggttg aaagatattg aaagcgctcg gttgcagcat aaccaggcgt ataccgaagc
    27181 catgcttacc gagtatgcgg attttttccg ccaggtcgag tcttcaccgc tgaatccggc
    27241 gcaggcccgg gcagtcgtta atggcgagca ttctctgtta gtgctggcag gtgcaggaag
    27301 cggaaaaacg tcggtgctgg tggcccgtgc aggctggttg ctggcgcgtg gtgaagcgtc
    27361 ccctgagcaa attttattgc tggcgtttgg tcgcaaagcc gctgaagaga tggacgagcg
    27421 gattcgcgaa cggctacata ccgaagacat taccgcacgc acgtttcatg cgctggcgct
    27481 gcatattatt cagcagggca gcaaaaaagt tccgatagtc agcaaactgg aaaatgatac
    27541 cgctgcccgt catgaactct ttattgctga gtggcgcaag caatgcagcg aaaagaaagc
    27601 gcaggcgaag ggctggcggc aatggctgac ggaagaaatg cagtggtcag tgccagaagg
    27661 taacttctgg gatgatgaaa aattacagcg tcgccttgcc tctcgcctcg atcgctgggt
    27721 aagtctgatg cggatgcacg gtggtgcaca ggcagaaatg attgccagtg cacccgaaga
    27781 gattcgcgat ctgttcagta aacgtatcaa gttgatggcc ccgttattaa aagcctggaa
    27841 aggtgcgctg aaggcagaaa acgctgtcga tttttcgggc cttattcatc aggcgattgt
    27901 gattctggag aaaggtcgct ttatcagccc gtggaagcat attctggttg atgaatttca
    27961 ggatatctcg tcgcagcggg cagcgttgtt agcggcatta cgcaagcaaa acagtcagac
    28021 gacgttgttc gctgttggtg atgactggca ggcgatttac cgattcagcg gtgcgcaaat
    28081 gtcgctcacc accgctttcc atgaaaactt tggtgaaggc gaacgttgtg atttagacac
    28141 gacttaccgt tttaacagtc gtatcggtaa ggtggcaaac cggtttattc agcagaaccc
    28201 aggccagctg aaaaagccgc taaacagctt aaccaatgga gacaaaaaag ccgtcacgtt
    28261 attggatgag agtcaacttg acgctttgct ggataagctc tctggttatg ccaaaccgga
    28321 agagcgcatt ctgatcctgg cgcgttacca tcacatgagg cctgccagcc tggaaaaagc
    28381 ggcaacacgc tggccgaagt tgcaaatcga ctttatgacc attcatgcca gcaaagggca
    28441 acaggcggat tacgtcatca tcgttggctt gcaggaggga agtgatggtt ttccggctgc
    28501 ggcgcgggag tcgattatgg aagaggcgct actgccaccg gttgaggatt tcccggacgc
    28561 tgaagaacgg cggttaatgt acgtggcgct gacccgggca cgccatcggg tatgggcact
    28621 gtttaacaaa gagaatccct ctccctttgt ggaaatactg aaaaatctgg atgtgccggt
    28681 ggcgagaaaa ccgtaagaaa caggtggcgt ttgccacctg tgcaatatta cttcagacgg
    28741 tccgcgagat aacgctgata atcggggatt agaatatcga ccgcgtcgtt gaaatgcggc
    28801 gactggatta tgaagtctgc cgttgccacg ttggtggcga ccggaatgtt ccataccgtc
    28861 gccagacgca gcaaggcttt cacgtcagga tcgtgcggca cggcatttag tggatcccag
    28921 aagaaaatca atacatcaat tttcccttct gagatcaatg cgccaacctg ctggtcaccc
    28981 cccattgggc cactcaacat cgcgttgacg ttcatgccgg tcgcgcggga aattaagtta
    29041 ccggtagtgc ctgttgcata cagtacgtgt tgttccagta acggttgatg ccgttccacc
    29101 cagctcatca gcatttgttt gcagtgatcg tgtgccacca gcgcaatatg tttccgcgca
    29161 ggtaaagtgc gagtcgtcag ttccataatg tacatccgta gttaactttc ctacagatta
    29221 ctgtaagcac ttatcgctgc aagataaaga ccgaaaaagc ctgcgcacag gcacaaaaat
    29281 ctcaggaaga tggttgtttt tccgcccact gcaggaaagt atttcgcgtt tgtgggtcag
    29341 ccagtttaaa ccaatacttc agccgttgtt ctgtgagcac ctgagactgc ggtggaatag
    29401 catccagctc ggaaacaccc gaaagcagcg tactgtcatc tgccatcatc gtggcaaatt
    29461 gcggtagtga agcgcgtttc gcggatttgt tatagcgttc aacctcgacc tcataatcaa
    29521 tcgtttttgc cgttgaggta atggcgagaa tatccagctt taccggaatc ggcgtcgcat
    29581 cgccatccaa caattcaagg cgcggcgcgg catcaaaatg gttagcttcc cgctcattct
    29641 ccaggcgagg caggcgaaaa ttgacctggt tgatgagctg ggtattaaaa ctgaccacca
    29701 gcggtgggga gatatacagc cgttcttcac tattggaaag atgaatcgtc ttctcaacgc
    29761 gaaacactaa ctgatgcggt ccattatcca gttcaatgct gtccgcaccg cgcagcagag
    29821 agctggaaac cttcttgccg tcgagtacca gaagatcgac atcggttgaa agccgcag
//
LOCUS       DAJVRF010000037        29549 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJVRF010000037 DAJVRF010000000
VERSION     DAJVRF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.037"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..690
                     /gene="dgt"
                     /locus_tag="OQN13_001519"
     CDS             <1..690
                     /gene="dgt"
                     /locus_tag="OQN13_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HCS1614906.1"
                     /translation="LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSR
                     SNSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDL
                     LKLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKR
                     FPIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAW
                     DEYRRLMAVEQ"
     gene            820..2244
                     /gene="degP"
                     /locus_tag="OQN13_001520"
     CDS             820..2244
                     /gene="degP"
                     /locus_tag="OQN13_001520"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCS1614907.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2399..3556
                     /gene="cdaR"
                     /locus_tag="OQN13_001521"
     CDS             2399..3556
                     /gene="cdaR"
                     /locus_tag="OQN13_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCS1614908.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3645..4031)
                     /locus_tag="OQN13_001522"
     CDS             complement(3645..4031)
                     /locus_tag="OQN13_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCS1614909.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4193..5005)
                     /gene="yaeI"
                     /locus_tag="OQN13_001523"
     CDS             complement(4193..5005)
                     /gene="yaeI"
                     /locus_tag="OQN13_001523"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HCS1614910.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5060..5884)
                     /gene="dapD"
                     /locus_tag="OQN13_001524"
     CDS             complement(5060..5884)
                     /gene="dapD"
                     /locus_tag="OQN13_001524"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCS1614911.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5915..8587)
                     /gene="glnD"
                     /locus_tag="OQN13_001525"
     CDS             complement(5915..8587)
                     /gene="glnD"
                     /locus_tag="OQN13_001525"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCS1614912.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8649..9443)
                     /gene="map"
                     /locus_tag="OQN13_001526"
     CDS             complement(8649..9443)
                     /gene="map"
                     /locus_tag="OQN13_001526"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCS1614913.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFIEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9811..10536
                     /gene="rpsB"
                     /locus_tag="OQN13_001527"
     CDS             9811..10536
                     /gene="rpsB"
                     /locus_tag="OQN13_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCS1614914.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10794..11645
                     /gene="tsf"
                     /locus_tag="OQN13_001528"
     CDS             10794..11645
                     /gene="tsf"
                     /locus_tag="OQN13_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCS1614915.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11792..12517
                     /gene="pyrH"
                     /locus_tag="OQN13_001529"
     CDS             11792..12517
                     /gene="pyrH"
                     /locus_tag="OQN13_001529"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCS1614916.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12809..13366
                     /gene="frr"
                     /locus_tag="OQN13_001530"
     CDS             12809..13366
                     /gene="frr"
                     /locus_tag="OQN13_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCS1614917.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13458..14654
                     /gene="ispC"
                     /locus_tag="OQN13_001531"
     CDS             13458..14654
                     /gene="ispC"
                     /locus_tag="OQN13_001531"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCS1614918.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14843..15601
                     /gene="ispU"
                     /locus_tag="OQN13_001532"
                     /gene_synonym="uppS"
     CDS             14843..15601
                     /gene="ispU"
                     /locus_tag="OQN13_001532"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCS1614919.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15614..16471
                     /gene="cdsA"
                     /locus_tag="OQN13_001533"
     CDS             15614..16471
                     /gene="cdsA"
                     /locus_tag="OQN13_001533"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCS1614920.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16483..17835
                     /gene="rseP"
                     /locus_tag="OQN13_001534"
     CDS             16483..17835
                     /gene="rseP"
                     /locus_tag="OQN13_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCS1614921.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17865..20297
                     /gene="bamA"
                     /locus_tag="OQN13_001535"
     CDS             17865..20297
                     /gene="bamA"
                     /locus_tag="OQN13_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCS1614922.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20419..20904
                     /gene="skp"
                     /locus_tag="OQN13_001536"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20419..20904
                     /gene="skp"
                     /locus_tag="OQN13_001536"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCS1614923.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20908..21933
                     /gene="lpxD"
                     /locus_tag="OQN13_001537"
     CDS             20908..21933
                     /gene="lpxD"
                     /locus_tag="OQN13_001537"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCS1614924.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22038..22493
                     /gene="fabZ"
                     /locus_tag="OQN13_001538"
     CDS             22038..22493
                     /gene="fabZ"
                     /locus_tag="OQN13_001538"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCS1614925.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22497..23285
                     /gene="lpxA"
                     /locus_tag="OQN13_001539"
     CDS             22497..23285
                     /gene="lpxA"
                     /locus_tag="OQN13_001539"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCS1614926.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23285..24433
                     /gene="lpxB"
                     /locus_tag="OQN13_001540"
     CDS             23285..24433
                     /gene="lpxB"
                     /locus_tag="OQN13_001540"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCS1614927.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24430..25026
                     /gene="rnhB"
                     /locus_tag="OQN13_001541"
     CDS             24430..25026
                     /gene="rnhB"
                     /locus_tag="OQN13_001541"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCS1614928.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25063..28545
                     /gene="dnaE"
                     /locus_tag="OQN13_001542"
     CDS             25063..28545
                     /gene="dnaE"
                     /locus_tag="OQN13_001542"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCS1614929.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28558..29517
                     /gene="accA"
                     /locus_tag="OQN13_001543"
     CDS             28558..29517
                     /gene="accA"
                     /locus_tag="OQN13_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCS1614930.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7159 a         7121 c         8160 g         7109 t
ORIGIN      
        1 cttgaagatg cggtagagaa aagaatattt accgttgagc agctttatca tcatttgcac
       61 gaggcgtggg gtcagcatga gaaaggttcg ctcttttcgc tagtggttga aaatgcctgg
      121 gaaaaatcac gctcaaatag tttaagccgc agtacggaag atcagttttt tatgtattta
      181 cgggtaaaca ccctaaataa actggtaccc tacgcggcac aacgatttat tgataatctg
      241 cctgcgattt tcgccggaac gtttaatcat gcattattgg aagatgccag cgaatgcagc
      301 gatcttctta agctatataa aaatgtcgct gtaaaacatg tgtttagcca tccggatgtc
      361 gagcagcttg aattgcaggg ctatcgggtc attagcggat tattagagat ttatcgtcct
      421 ttattaagcc tgtcgttatc agactttact gaactggtag aaaaagaacg ggtgaaacgt
      481 ttccctattg agtcgcgctt attccacaaa ctctcgacgc gccatcggct ggcctatgtc
      541 gaggctgtca gtaaattacc gtcagattct cctgagtttc cgctatggga atattattac
      601 cgttgccgcc tactgcagga ttatatcagc ggtatgaccg acctctatgc gtgggatgaa
      661 taccgacgtc tgatggccgt agaacaataa ccaggctttt gtaaagacga acaataaatt
      721 tttacctttt gcagaaactt tagttcggaa cttcgcgcta taaaacgaat ctgaagaaca
      781 cagcaatttt gcgttatctg ttaatcgaga ctgaaataca tgaaaaaaac cacattagca
      841 ctgagtgcac tggctctgag tttaggtttg gcgttatctc cgctctctgc aacggcggct
      901 gagacttctt cagcaacgac agcccagcag atgccaagcc ttgcaccgat gctcgaaaag
      961 gtgatgcctt cagtggtcag cattaacgta gaaggtagca caaccgttaa tacgccgcgt
     1021 atgccgcgta atttccagca gttcttcggt gatgattctc cgttctgcca ggaaggttca
     1081 ccgtttcaga gctctccgtt ctgccagggg ggccagggcg gtaatggcgg cggccagcaa
     1141 cagaaattca tggcgctggg ttccggcgtc atcattgatg ccgataaagg ctatgtcgtc
     1201 accaacaacc atgttgttga taacgcgacg gtgattaaag tccagctgag cgatggtcgt
     1261 aagttcgacg cgaagatggt tggcaaagat ccgcgctctg atatcgcgct gatccaaatc
     1321 cagaacccga aaaacctgac cgcaattaag atggcggatt ctgatgcact gcgcgtgggt
     1381 gattacaccg tagcgattgg taacccgttt ggtctgggcg agacggtaac ttccgggatt
     1441 gtctctgcgc tggggcgtag cggcctgaat gctgaaaact acgaaaactt catccagacc
     1501 gatgcagcga tcaaccgtgg taactccggt ggtgcgctgg ttaacctgaa cggcgaactg
     1561 atcggtatca acaccgcgat cctcgcaccg gacggcggca acatcggtat cggttttgct
     1621 atcccgagta acatggtgaa aaacctgacc tcgcagatgg tggaatacgg ccaggtgaaa
     1681 cgcggtgagc tggggattat ggggactgag ctgaactccg aactggccaa agcgatgaaa
     1741 gttgacgccc agcgcggtgc tttcgtaagc caggttctgc ctaattcctc cgctgcaaaa
     1801 gcgggcatta aagcgggtga tgtgatcacc tcactgaacg gtaagccgat cagcagcttt
     1861 gccgcactgc gtgctcaggt gggtactatg ccggtgggca gcaaactgac cctgggctta
     1921 ctgcgcgacg gtaagcaggt taacgtgaac ctggaactgc agcagagcag ccagaatcag
     1981 gttgattcca gctccatctt caacggcatt gaaggcgctg agatgagcaa caaaggcaaa
     2041 gatcagggcg tggtagtgaa caacgtgaaa acgggcactc cggctgcgca gatcggcctg
     2101 aagaaaggtg atgtgattat tggcgcgaac cagcaggcag tgaaaaacat cgctgaactg
     2161 cgtaaagtgc tcgacagcaa accgtctgtg ctggcactga acattcagcg cggcgacagc
     2221 accatctacc tgttaatgca gtaatctccc tcaacccctt cctgaatacg ggaaggggtt
     2281 ctccttacaa tctgtgaact tcgccacaac tccatacatc ttcatcatcc tttgggcatt
     2341 tgcacaatgc cgtacgttac gtacttcctt atgctaagcc gtgcataacg gaggacttat
     2401 ggctggctgg catcttgata ccaaaatggc gcaggatatc gtggcacgta ccatgcgcat
     2461 catcgatacc aatatcaacg taatggatgc ccgcgggcga attatcggca gcggcgatcg
     2521 tgagcgtatt ggtgaattgc acgaaggtgc attgctggta ctttcacagg gacgagtcgt
     2581 cgatatcgat gacgcggtag cacgtcatct gcacggtgtg cggcagggga ttaatctacc
     2641 gttacggctg gaaggtgaaa ttgtcggcgt aattggcctg acaggtgaac cagagaatct
     2701 gcgtaaatat ggcgaactgg tctgcatgac ggctgaaatg atgctggaac agtcgcggtt
     2761 gatgcacctg ttggcgcagg atagccgttt gcgggaagaa ctggtgatga acctgattca
     2821 ggctgaggag aatactcccg cacttactga atgggcgcaa cggctgggga ttgatctcaa
     2881 tcaaccgcga gtggtggcta ttgttgaggt cgacagcggt cagcttggcg tggacagcgc
     2941 aatggcggag ttacaacaac tgcaaaatgc gctgactacg cccgagcgta ataatctggt
     3001 ggcaattgtt tcgctgaccg aaatggtggt gttgaaaccg gcgttgaact cttttgggcg
     3061 ctgggatgca gaagatcatc gtaagcgagt tgaacaactg attacccgca tgaaagagta
     3121 cggccagctg cgttttcgcg tttcactggg caactatttt accggtcctg gcagtattgc
     3181 ccgatcctat cgtacggcga aaacgacgat ggtggtgggt aaacagcgga tgccagaaag
     3241 tcgctgctat ttttatcagg atctgatgtt acctgtgtta ctcgacagtt tgcgtggcga
     3301 ctggcaggcc aacgaactgg cgcgaccgct ggcgcggctg aaagcgatgg acaataatgg
     3361 cttgctgcga cgaacgctgg cggcgtggtt tcgccacaat gtgcaaccgc tggctacgtc
     3421 aaaggcgctg tttattcatc gtaatactct ggagtatcgg cttaatcgta tatcggaact
     3481 gaccgggctt gatttgggta attttgatga caggttgctg ctgtatgtgg cgttacaact
     3541 ggatgaagag cggtaggtta tgcgttaagg tcgaggcggc gctggctcat tcccctcagc
     3601 ccgaagggta gaggggaatg tccgagttaa ccgtcagttc ggcattattt acgcgttagt
     3661 ttttccagat cggcttcaat ctcgctgatc ttattggtta cgactgactc caggtgacgt
     3721 aagtcgtcga ggatcttacg cttaagatca acttcgctgc ggtcacgctg gcagatttga
     3781 tcaagctcat caatgatata ccgtagattc gggctgattt cctggacttc tttataaccc
     3841 tgacccacac catcagcgac gaccgtttta cgctgacgcg gatatttaaa cttcacgctc
     3901 ttggcgaaaa actcgccttt gtctttctgg aaatagattt tcagaatatc gttgttggct
     3961 tcctgccgga ggctgtaacg atcaatttct tcaggattgg taatacccag acttttcaga
     4021 ttgtcgtaca tagcgttacc tcaaaatgag tcagtaaatt gtgcttattt tagcatttgg
     4081 catggcccgc ccggctcgac tgtgatcgca gaaagccttt ttcttccaga cttctatcat
     4141 ggcgtaaaaa tcaaaaaatt acctgcttta ttgtggtgat aaaattcacg ttctacacca
     4201 gttccagcat cgttacttcc gggcggcagt tcagacgcaa accatacaaa ctgcccacgc
     4261 cacgggttgt gtagatgtgt cgttcgccaa aggcatttaa tccggcgacg taacgtttat
     4321 cttcgacagg ggcaaaaggt tcaccaacca acggtacgcg cagttgcccg ccgtgagtat
     4381 ggccgcacag catcagatcc cagggttcat cacgcatgac ttctttgctg tcgggattat
     4441 gtgccagcat cagtcttggc agatttgctt cgctggcggg aggtggtttg cattgtccgg
     4501 cccataagtc accggtgcct accagttcga attgcctgtt cggcgtggcg atcaccgtag
     4561 cctggttaaa caacaccgtg atgcccactg atttcaacgt ctcgccaatt aagtgatttt
     4621 tttctgtacc aacagggcga tcgtggttgc cgaagcaggc aaacgtcggc gcacattcgg
     4681 caagggggga gaggacatca ctaaacgccg aaaaattcag cggcatatca aataatacgt
     4741 aatcgccgcc cagtaatatc aaatcgggct tttgttctat gccaagagca atcgcgtcag
     4801 aaatcaggct taaaggaaca aaacgagagt aatggagatc ggccagaaaa agaattttga
     4861 atggtgctgc gttgtcttta aaaaaggcga ggcggtgacg gattaattca aaccagccta
     4921 gctcccagta atgcatataa ccgaaacctg agctcgtggc tatcgtcgcg gcagtagcct
     4981 gcaacaatcg gcggcgtgaa atcatcgctc atccctgcaa aaaaaatcgg gcagcgtcgt
     5041 gctgcccgtg tgcatacttt tagtcgatgg tacgcagcag ttcgttaatg ccgactttgc
     5101 cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca gtagaggctg tatttgccat
     5161 cttttgacgg cagattacct gaaacaacca cagaccccgc cggaacgcga ccgtagtgga
     5221 tttcgccggt ttcacggtcg taaatacggg tgctctgacc aatgtatacg cccatggaaa
     5281 tgacggaacc ttcttcgaca atcacccctt caaccacttc agagcgtgcg ccaatgaagc
     5341 agttatcttc aatgatggtt gggttagcct gcagcggttc cagcacgccg ccgatgccca
     5401 cgccaccgga aaggtggacg tttttaccaa tctgcgcaca agaaccgacg gtcgcccagg
     5461 tatcaaccat ggtgccttca tcaacatatg cgccgatgtt gacgtaagac ggcatcagca
     5521 cggtgttacg ggcaataaac gcaccctgac gtaccgccgc tggtggcaca acgcggaagc
     5581 cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt catcggcact ttgtcgaagt
     5641 agcggctttc tgccccttcg atcacctgat tatcattaat acggaaagag agcagcaccg
     5701 cttttttcaa ccactgatgc gtcacccact gaccgtcaat tttttccgct acacgcagtg
     5761 caccggaatc cagcagggcg atcacctgat ttaccgcttc gcgggtaacg gtgtctgcgt
     5821 tggctggcgt gatctcggca cggcgttcaa aagcggtttc aataatgttc tgtaactgct
     5881 gcattgttaa actcttttca tatcagtaaa cacatcaccc tttatcgttt ggattgaggg
     5941 cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt attaagtgca cgccggtcag
     6001 cggtggcaat tatgaataaa tcttctactc gctcgccaat ggttgtaatt cgggcaccat
     6061 gaagcgaaat tcccagatcg gcaaaaattt tcccgactct cgccagcagt cctggctggt
     6121 cgagcgcgat aagctcaagg aacgatttgc ggtcggtatg ggttggcaaa aacgtcactt
     6181 cggtttcaac agtaaagtga cgtagtttag ccggttgacg acgcggctgt ggtggctgcc
     6241 agctactttg tgtcagtacc tgctccagac caaaccgaat aacctcatga cgatctgcgg
     6301 atagcgggct accatcgggt tccagcacga tgaaggtatc catcgccata ccgtcgcgag
     6361 tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg gtctaattcg gcacagacgg
     6421 cggcaaacag ataggggcgg tccgggctcc agataaaaat ctcggtgcct ccacgcgtag
     6481 cctgcgggct aagcaatacc agcggtttgc ttaaatcatg ctgtaataaa tggcgggcat
     6541 gccaggccag ttgatttggg ctatggcgga caaaatagtt agcacgacag cgtgaccaaa
     6601 tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag tagtgccagt gcctggagtt
     6661 ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg cattccgcgt cgtagctgct
     6721 tttcggtggc aaaatagagt tcgcgcaaca gactttgctt ccagctattc cacagcgttt
     6781 cgttggtggc gcaaatgtca gccactgtca ggcataccag atagtgcaga cgattttccg
     6841 tttgaacttc ttcggcaaac tgcttgatga cttcagggtc ctgaatatcg cggcgttgtg
     6901 ctgtcaccga catcagcaag tgctggcgaa ccagccaggc aaccagctgt gtttcgcgtg
     6961 agttcagccc gtggagttct gcaaaatgca ctacatcctg agcaccgaga atggagtggt
     7021 cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc gatgaaaatc agctcagttg
     7081 acggcaggcg cggccagacg tccacacaca acggatggcg ctggcgcgtt tcttcactgg
     7141 caaaactctc cagtttcagc atcacgcgga tagtatgttc atccaccgtg taggcgtgga
     7201 acagatcaaa ctgcatctgc ccgacgatat gcgaccattg cggcatatac gcgccgagca
     7261 cgctatggcg atgcattggc aatagcccgc gccgcaccgc tccggggtga cgcagaatgc
     7321 tcaaaaacag ttttcgtgct tccggaatat tacacagcgg ttgttgcaga tggcgacggg
     7381 catggcgtaa ctggcgcagc gtggtggagt aaatgccggt gatcgcactg ttgcgcacca
     7441 tggtgtagaa catacgcaag atggcttccg gctggcgcat aaatagtgtt tcatcacgca
     7501 ggtcgattag cgtaccgcgt agctgaaact catcgtcgat tggacgtggt ttttcgtcgg
     7561 cgggaagggc gaggatggct tcatcgaaca gttgcagcag catctggttg agttcactga
     7621 cgcggcgtgt aacgcggaag taatccttca tcatccgctc gaccggttcg ttgccttcac
     7681 cactgtaatt cagacgctga gcgacgctaa gttggcgatc gaataacagg cgattatcgt
     7741 aacggctgac gaccagatgc agggcaaagc gaatacgcca cagtatatgc agacattcgt
     7801 ttaattccgc ccgctccgct gaggttaaga agccaaaccc aaccatttca tccagcgatg
     7861 ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg gatatcgcgc aagccgccag
     7921 ggctactttt gatgtctggt tcaagattgt agctggtgcc gtggtaacgc tgatggcgct
     7981 ggttctgttc ttcaactttc gccgcgtaga acttgtcgga aggccagaat ccttcgctaa
     8041 aaatatgttt ttgcagttcg aggaagagcg caacatcgcc aattaataag cgggattcga
     8101 ttaaattggt ggcgacggtt aaatccgata acccttccag catgcactct tcaagcgtgc
     8161 gcacactatg accgacttcc agctttacat cccagagcag cgttaacagc tcgcccactt
     8221 tttgcgcctg atcgtccggg agctttttac ggcttaaaat cagtaaatcg atgtcagaaa
     8281 gtggatgcag ctcgccacga ccgtagccac cgacggcgac caatgccagg tcggcaatct
     8341 ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg gtcgataaac tcggtgcgcg
     8401 cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc atcacccagc caacgctgga
     8461 aagtatcgat atgggctttt atcccaccga cggttaattc atcacggggc cagacgcatg
     8521 gattttgcgg ttgaccgggc agggtgggga gagcggtgtt tgcgtactgt tctggaaggg
     8581 tattcattgt gcgccaccca taagattaaa ttatcgcatt aaaaaagccg gcattcgccg
     8641 gcttcatctt attcgtcgtg cgagattatc gccgggatgg tgtcatcctt gcgtagcgtc
     8701 agaatttcgc agccgttatc agtcaccaca atagtatgct catattgtgc agacaagctg
     8761 cgatctttgg tttttaccgt ccagccatct ttcatggtgc ggatctcttt tttacccgcg
     8821 ttgaccattg gctcgatggt gaacgtcatc ccaggtttca gtacgacgtt ggtttcacgg
     8881 gagtcatagt gcagcacctg cggttcttca tggaagccgc gaccaatacc gtgtccgcaa
     8941 tattcacgaa cgacggagaa gccttctgct tcgataaact tctgaatcgc cgcaccgatt
     9001 tcgcgcaggt taatgcctgg tttgaccatg cgcagcgcca ggtacaggct ttcttgcgtg
     9061 atgcggcaca gacgttcgcc catgatggtc ggcttaccga cgataaacat tttcgaggta
     9121 tcgccgtgga aaccatcttt gattacggtg acatcaatgt taacgatatc gccatctttc
     9181 agcagcttcg catcgtccgg gataccgtgg cacaccactt cattaataga gatgcaaacg
     9241 gatttcggat agccgtgata gccgaggcag gcagaaaccg cgtgttgttc attaacaatg
     9301 taatcattac agatgcgatc cagctcgccg gtgctgacgc ccggtttaac atacggttcg
     9361 atcatctcca gcacttcggc agccagtcgg ccagcgacgc gcattttttc gatatcttct
     9421 ggggtcttga ttgagatagc cattaattct gtccatcagc gtcggtgata ccgacaatat
     9481 atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt gagaatcatt ctgaatttcg
     9541 ccaaacgtgc cactgaacgt tttctataat agaaaattca acgtctgatg ctgtacacag
     9601 cgccaacaat tattggtgtc catgacgtat ttgtggtata aagcgcgccg gacttccgat
     9661 ccatttcgta tacacagact ggacggaagc gacaatctca ctttgtgtaa caacacacac
     9721 gtatcggcac atattccggg gtgccctttg gggtcggtaa tatgggatac gtggaggcat
     9781 aaccccaact tttatataga ggttttaatc atggcaactg tttccatgcg cgacatgctc
     9841 aaggctggtg ttcacttcgg tcaccagacc cgttactgga acccgaaaat gaagccgttc
     9901 atcttcggtg cgcgtaacaa agttcacatc atcaaccttg agaaaactgt accgatgttc
     9961 aacgaagctc tggctgaact gaacaagatt gcttctcgca aaggtaaaat ccttttcgtt
    10021 ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg ctctgagctg cgaccagttc
    10081 ttcgtgaacc atcgctggct gggcggtatg ctgactaact ggaaaaccgt tcgtcagtcc
    10141 atcaaacgtc tgaaagacct ggaaactcag tctcaggacg gtactttcga aaagctgacc
    10201 aagaaagaag cgctgatgcg cactcgtgag ctggagaaac tggaaaacag cctgggcggt
    10261 atcaaagaca tgggcggtct gccggacgct ctgtttgtaa tcgatgctga ccacgaacac
    10321 attgctatca aagaagcaaa caacctgggt attccggtat ttgctatcgt tgataccaac
    10381 tctgatccgg acggtgttga cttcgttatc ccgggtaacg acgacgcaat ccgtgctgtg
    10441 accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag gccgttctca ggatctggct
    10501 tcccaggcgg aagaaagctt cgtagaagct gagtaataag gcttgataac tcccccaaaa
    10561 tagttcgagt tgcagaaagg cggcaagctc gagaattccc gggagcttac atcagtaagt
    10621 gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg aaatatgaag ggggagagcc
    10681 cttatagacc aggtagtaca cgtttggtta gggggcctgc atatggcccc ctttttcact
    10741 tttatatctg tgcggtttaa tgccgggcag atcacatctc cgaggatttt agaatggctg
    10801 aaattaccgc atccctggta aaagagctgc gtgagcgtac tggcgcaggc atgatggatt
    10861 gcaaaaaagc actgactgaa gctaacggcg acatcgagct ggcaatcgaa aacatgcgta
    10921 agtccggtgc tattaaagca gcgaaaaaag caggcaacgt tgctgctgac ggcgtgatca
    10981 aaaccaaaat cgacggcaac tacggcatca ttctggaagt taactgccag actgacttcg
    11041 ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt tctggacgca gctgttgctg
    11101 gcaaaatcac tgacgttgaa gttctgaaag cacagttcga agaagaacgt gttgcgctgg
    11161 tagcgaaaat tggtgagaac atcaacattc gccgcgttgc tgcgctggaa ggcgacgttc
    11221 tgggttctta tcagcacggt gcgcgtatcg gcgttctggt tgctgctaaa ggcgctgacg
    11281 aagagctggt taaacacatc gctatgcacg ttgctgcaag caagccagaa ttcatcaaac
    11341 cagaagacgt atccgctgaa gtggtagaaa aagaatacca ggtacagctg gatatcgcga
    11401 tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt tgaaggccgc atgaagaaat
    11461 tcaccggcga agtttctctg accggtcagc cgttcgttat ggaaccaagc aaaactgttg
    11521 gtcagctgct gaaagagcat aacgctgaag tgactggctt catccgcttc gaagtggggg
    11581 aaggcatcga gaaagttgag actgactttg cagcagaagt tgctgcgatg tccaagcagt
    11641 cttaattatc gaaaaggagc cgcctgaggg cggcttcttt ttgtgcccat cttgtaaatt
    11701 cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca atgtcgctag tattaattca
    11761 tttcaatcgt tgacagtctc aggaaagaaa catggctacc aatgcaaaac ccgtctataa
    11821 acgcattctg cttaagttga gtggcgaagc tctgcagggc actgaaggct tcggtattga
    11881 tgcaagcata ctggatcgta tggctcagga aatcaaagaa ctggttgaac tgggtattca
    11941 ggttggtgtg gtgattggtg ggggtaacct gttccgtggc gctggtctgg cgaaagcggg
    12001 tatgaaccgc gttgtgggcg accacatggg gatgctggcg accgtaatga acggcctggc
    12061 aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt ctgatgtccg ctattccatt
    12121 gaatggcgtg tgcgacagct acagctgggc agaagctatc agcctgttgc gcaacaaccg
    12181 tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt accaccgact cagcagcttg
    12241 cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa gcaaccaaag ttgacggcgt
    12301 gtttaccgct gatccggcga aagatccaac cgcaaccatg tacgagcaac tgacttacag
    12361 cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg gccttcacgc tggctcgtga
    12421 ccataaatta ccgattcgtg ttttcaatat gaacaaaccg ggtgcgctgc gccgtgtggt
    12481 aatgggtgaa aaagaaggga ctttaatcac ggaataattc ccgtgatgga taaataaggg
    12541 taagattccg cgtaagtatc gcgggggcgt aagtctggtt ataaggcgtt attgttgcag
    12601 gcagtttggt cacggccagc gcgcagcaac cggagcgtac aaaagtacgt gaggatggcg
    12661 agcactgctc ggggccaaaa tggcaaataa aatagcctaa taatccagac gattacccgt
    12721 aatatgttta atcagggcta tacttagcac acttccactg tgtgtgactg tctggtctga
    12781 ctgagacaag ttttcaagga ttcgtaacgt gattagcgat atcagaaaag atgctgaagt
    12841 acgcatggac aaatgcgtag aagcgttcaa aacccaaatc agcaaaatac gcacgggtcg
    12901 tgcttctccc agcctgctgg atggcattgt cgtggaatat tacggcacgc cgacgccgct
    12961 gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact ctgaaaatca acgtgtttga
    13021 tcgttcaatg tctccggccg ttgaaaaagc gattatggcg tccgatcttg gcctgaaccc
    13081 gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg ctgacggaag aacgtcgtaa
    13141 agatctgacc aaaatcgttc gtggtgaagc agaacaagcg cgtgttgcag tacgtaacgt
    13201 gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa gataaagaga tcagcgaaga
    13261 cgacgatcgc cgttctcagg acgatgtaca gaaactgact gatgctgcaa tcaagaaaat
    13321 tgaagcggcg ctggcagaca aagaagcaga actgatgcag ttctgatttc ttgaacgaca
    13381 aaaacgccgc tcagtagatc cttgcggatc ggctggcggc gttttgcttt ttattctgtc
    13441 tcaactctgg atgtttcatg aagcaactca ccattctggg ctcgaccggc tcgattggtt
    13501 gcagcacgct ggacgtggtg cgccataatc ccgaacactt ccgcgtagtt gcgctggtgg
    13561 caggcaaaaa tgtcactcgc atggtagaac agtgcctgga attctctccc cgctatgccg
    13621 taatggacga tgaagcgagt gcgaaacttc ttaaaacgat gctacagcaa cagggtagcc
    13681 gcaccgaagt cttaagtggg caacaagccg cttgcgatat ggcagcgctt gaggatgttg
    13741 atcaggtgat ggcagccatt gttggcgctg ctgggctgtt acctacgctt gctgcgatcc
    13801 gcgcgggtaa aaccattttg ctggccaata aagaatcact ggttacctgc ggacgtctgt
    13861 ttatggacgc cgtaaagcag agcaaagcgc aattgttacc ggtcgatagc gaacataacg
    13921 ccatttttca gagtttaccg caacctatcc agcataatct gggatacgct gaccttgagc
    13981 aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg ccctttccgt gagacgccat
    14041 tgcgcgattt ggcaacaatg acgcccgatc aagcctgccg tcatccgaac tggtcgatgg
    14101 gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa aggtctggaa tacattgaag
    14161 cgcgttggct gtttaacgcc agcgccagcc agatggaagt gctgattcac ccgcagtcag
    14221 tgattcactc aatggtgcgc tatcaggacg gcagtgttct ggcgcagctg ggggaaccgg
    14281 atatgcgtac gccaattgcc cacaccatgg catggccgaa tcgcgtgaac tctggcgtga
    14341 agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc cgcaccggat tatgatcgtt
    14401 atccatgcct gaaactggcg atggaggcgt tcgaacaagg tcaggcagcg acgacagcat
    14461 tgaatgccgc aaacgaaatc accgttgctg cttttcttgc gcaacaaatc cgctttacgg
    14521 atatcgctgc gttgaattta tctgtactgg aaaaaatgga tatgcgcgaa ccacaatgtg
    14581 tggacgatgt gttatctgtt gatgcgaacg cgcgtgaagt cgccagaaaa gaggtgatgc
    14641 gtctcgcaag ctgaggataa tccggctaca gagagtcgcg ctatttgtta gcgtagggct
    14701 tcagtgatat agtctgcgcc atctgatcgt aagtagttgg ctttataagg tcaggtatgc
    14761 cgtggtttta cacggctttt ttttgtatag gcttcagtat tcctgagtac cgtaaaccct
    14821 gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa ccacttagcg aaaaattgcc
    14881 agcgcatggc tgccgtcatg ttgcgatcat tatggacggc aatggtcgct gggcaaaaaa
    14941 gcaagggagg attcgtgcct ttgggcataa agccggggca aaatccgtcc gccgggctgt
    15001 ctcttttgcg gccaacaacg gtattgaggc gttaacgctg tatgccttta gtagtgaaaa
    15061 ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa ctgtttgtgt gggcgctcga
    15121 tagcgaagta aaaagtctgc accgacataa cgtgcgtctg cgtattattg gcgataccag
    15181 tcgctttaac tcgcgtttgc aagaacgtat tcgtaaatct gaagcgctaa cagctgggaa
    15241 taccggtctg acgctgaata ttgcggcgaa ctacggtgga cgttgggata tagtccaggg
    15301 agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg caaccagatc agatagatga
    15361 agagatgcta aaccagcatg tctgtatgca tgaactggcc cctgtagatt tagtaattag
    15421 gactgggggg gagcatcgca ttagtaactt tttgctttgg caaattgcct atgccgaact
    15481 ttactttaca gatgttctct ggcctgattt cgatgaacaa gactttgaag gggcgttaaa
    15541 tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag cccggtgatg aaacagcctg
    15601 atggggggcg cttttgctga agtatcgcct gatatctgct tttgtgttaa tacccgtcgt
    15661 catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc atcgtaacgc tggtggtctg
    15721 catgctggca gcgtgggaat ggggacagct tagcggtttt accactcgtt cgcagcgagt
    15781 atggttggcg gtgttatgcg ggttattgtt ggcgctgatg ctttttctgt tgccggaata
    15841 tcaccgaaat attcatcaac cgctggttga aatctcactt tgggcttcgt tgggttggtg
    15901 gattgtcgcg ctattgctgg tgctgtttta cccaggttcc gcagcaatct ggcgtaactc
    15961 taaaacattg cgccttattt ttggcgtgct aaccattgtt cccttcttct ggggcatgct
    16021 ggcgttacgg gcctggcact atgacgagaa tcattacagt ggcgcaatat ggctgctcta
    16081 tgtcatgatc ctggtatggg gcgctgactc cggcgcatat atgtttggca aattgtttgg
    16141 taaacataag ctggcaccga aggtttctcc gggtaaaacc tggcaaggct ttataggtgg
    16201 actagcgacc gcagcggtaa tctcatgggg ttatggcatg tgggcgaatc tcgacgtcgc
    16261 tcccgtcacc ttactcattt gctctattgt cgcagcgtta gcctcagtgc tcggcgatct
    16321 gaccgagagt atgtttaagc gtgaagcagg aattaaggac agcggtcatt taattccagg
    16381 acacggtggt attttagatc gtattgatag cctgacggct gcggtaccgg tctttgcttg
    16441 cttgttgtta ctggtattca ggacgcttta acggaaggta atatgctgag ttttctctgg
    16501 gatttggctt cgttcatcgt tgcactgggt gtacttatca ccgtgcatga atttggtcat
    16561 ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt tctcaatagg gtttggtaag
    16621 gcgctctggc ggcgaactga taagctcggc accgaatatg ttatggccct gattccgctg
    16681 ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg tcgttccgga actccgccac
    16741 catgccttca ataataaatc tgttggccaa cgagcggcga ttattgccgc aggtccggtt
    16801 gcaaacttca tttttgctat ctttgcctac tggctggttt ttattattgg tgtgcctggc
    16861 gtacgtccgg tggttggtga aatagcagcc aattcgatag ctgcggaagc acaaattgca
    16921 ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc ctgattggga tgccgtgcgt
    16981 ttgcagttgg tcgataaaat tggcgatgaa agcaccacca ttacagtagc gccatttggc
    17041 agcgaccaac ggcgggatgt aaagctcgat ttacgtcact gggcgtttga gcctgataaa
    17101 gaagatccgg tatcttcgct ggggattcgt cctcgtgggc cgcaaattga acctgtactg
    17161 gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt tgcaagcagg cgacaggatc
    17221 gttaaagtcg atggtcagcc cttaacgcag tgggtgacct ttgtgatgct tgtccgggat
    17281 aacccgggta aatccttagc gttagaaatc gaaaggcagg ggagtccctt gtctttgaca
    17341 ttaatcccgg agagtaaacc gggtaatggt aaagcgattg gttttgtcgg tattgagccg
    17401 aaagtcattc ctttgccaga tgagtataaa gttgtacgcc agtatgggcc gttcaacgcc
    17461 atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc tgacggtcag tatgctggga
    17521 aaattgatca ccggtgatgt gaaactgaac aacctcagtg ggccgatctc tatcgccaag
    17581 ggggctggga tgacagcgga actcggggta gtttattacc tgccgtttct tgcgcttatt
    17641 agcgtgaact tagggataat taacctgttt ccgttgcccg tacttgacgg ggggcatctg
    17701 ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat ccgagcgggt tcaagacttt
    17761 tgttatcgca ttggctcgat tctgctggtg ctgttaatgg ggcttgcact tttcaatgat
    17821 ttctctcggt tatgagagtt agttaggaag aacgcataat aacgatggcg atgaaaaagt
    17881 tgctcatagc gtcgctgctg tttagcagcg ccaccgtata cggtgctgaa gggttcgtag
    17941 tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt tggtgcggca ctcctcagta
    18001 tgccggtgcg cacaggcgac acggttaatg atgaagatat cagtaatacc attcgcgctc
    18061 tgtttgctac cggcaacttt gaggatgttc gcgtccttcg tgatggtgat acccttctgg
    18121 ttcaggtaaa agaacgtccg accattgcca gcattacttt ctccggtaac aaatcggtga
    18181 aagatgacat gctgaagcaa aacctcgagg cttctggtgt gcgtgtgggc gaatccctcg
    18241 atcgcaccac cattgccgat atcgagaaag gtctggaaga cttctactac agcgtcggta
    18301 aatatagcgc cagcgtaaaa gctgtcgtga ccccgctgcc gcgcaaccgt gttgacctaa
    18361 aactggtgtt ccaggaaggt gtgtcagctg aaatccagca aattaacatt gttggtaacc
    18421 atgctttcac caccgacgaa ctgatctctc atttccaact gcgtgacgaa gtgccgtggt
    18481 ggaacgtggt aggcgatcgt aaataccaga aacagaaact ggcgggcgac cttgaaaccc
    18541 tgcgcagcta ctatctggat cgcggttatg cccgtttcaa catcgactct acccaggtca
    18601 gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa cattaccgaa ggcgatcagt
    18661 acaagctttc tggcgttgaa gtgagcggca accttgccgg gcactccgct gaaattgagc
    18721 agctgactaa gatcgagccg ggcgagctgt ataacggcac caaagtgacc aagatggaag
    18781 atgacatcaa aaagcttctc ggtcgctatg gttatgccta tccgcgcgta cagtcgatgc
    18841 ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt gaacgttgat gcgggtaacc
    18901 gtttctacgt gcgtaagatc cgttttgaag gtaacgatac ctcgaaagat gccgtcctgc
    18961 gtcgcgaaat gcgtcagatg gaaggtgcat ggctgggaag cgatctggtc gatcagggta
    19021 aggagcgtct gaatcgtctg ggcttctttg aaactgtcga taccgatacc caacgtgttc
    19081 cgggtagccc ggaccaggta gatgtagtct acaaggtgaa agaacgtaac accggtagct
    19141 tcaacttcgg gattggttac ggtactgaaa gtggcgtgag cttccaggct ggcgtgcaac
    19201 aggataactg gttaggtaca ggttatgctg ttggtatcaa cgggaccaaa aacgattacc
    19261 agacctatgc tgaattgtcg gtaaccaacc cgtacttcac tgtagatggt gtaagcctcg
    19321 gtggtcgtct cttctataat gacttccagg cagatgacgc cgacctgtcc gactatacca
    19381 acaagagtta tggtacagac gtgacgttgg gcttcccgat caacgaatat aactcgctgc
    19441 gtgcaggtct gggttatgta cataactccc tgtccaacat gcagcctcag gttgcgatgt
    19501 ggcgttatct gtactctatg ggtgaacatc cgagcacctc tgatcaggat aacagcttca
    19561 aaacggacga cttcacgttc aactatggtt ggacctataa caagcttgac cgtggttact
    19621 tcccgacaga tggttcacgt gtcaacctga ccggtaaagt gaccattcct ggatcggata
    19681 acgaatacta caaagtgacg ttagacacgg cgacttatgt gccgatcgat gacgatcaca
    19741 aatgggttgt tctggggcgt acccgctggg gttatggtga tggtttaggc ggcaaagaga
    19801 tgccgttcta cgagaacttc tatgccggtg gttccagcac cgtgcgtggc ttccagtcca
    19861 atacaattgg tccgaaagca gtttacttcc cgcatcaggc cagtaattat gatccggact
    19921 atgattacga atgtgcgact caggacggcg cgaaagacct gtgtaaatcg gatgatgctg
    19981 taggcggtaa cgccatggcg gttgccagcc tcgagttcat caccccgacg ccgtttatta
    20041 gcgataagta tgctaactcg gttcgtactt ccttcttctg ggatatgggt accgtttggg
    20101 atacaaactg ggattccagc caatattctg gatatccgga ctatagtgat ccaagcaata
    20161 tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc attggggccg ttggtgttct
    20221 cctacgccca gccgttcaaa aagtacgatg gagacaaggc agaacagttc cagtttaaca
    20281 tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag tggtagtgta gcgatgactt
    20341 taggcgatca atataagatc gccgggccac gcaaagaact gcaccctccg gtgcaaatgg
    20401 gatggtaagg agtttattgt gaaaaagtgg ttattagcgg caggtctcgg tttagcactg
    20461 gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca atatgggcag cctgttccag
    20521 caggtagcgc agaaaaccgg tgtttctaac acgctggaaa atgagttcaa aggccgtgcc
    20581 agcgaactgc agcgtatgga aaccgatctg caggctaaaa tgaaaaagct gcagtccatg
    20641 aaagcgggca gcgatcgcac taagctggaa aaagacgtga tggctcagcg ccagactttt
    20701 gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc gttccaacga agaacgcggc
    20761 aaactggtta ctcgtatcca gactgctgtg aaatccgttg ccaacagcca ggatatcgat
    20821 ctggttgttg atgcaaacgc cgttgcttac aacagcagcg atgtaaaaga catcactgcc
    20881 gacgtactga aacaggttaa ataagtaatg ccttcaattc gactggctga tttagcgcag
    20941 cagttggatg cagaactaca cggtgatggc gatatcgtca tcaccggcgt tgcgtccatg
    21001 caatctgcac aaacaggtca cattacgttc atggttaacc caaaataccg tgagcattta
    21061 ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg atcttccttt cgcgaaaagt
    21121 gccgcgctgg tagtgaagaa tccctacctg acttacgcgc gcatggcgca aattttagat
    21181 accacgccgc agcccgcgca gaacattgca cccagtgcgg tgatcgacgc gacggcgaag
    21241 ctgggtaaca acgtatcgat tggcgctaac gcggtgattg agtccggcgt tgaactgggc
    21301 gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa acagcaaaat cggtgcaggt
    21361 tcgcgtctct gggcgaacgt aaccatttac catgagatcc agatcggtca gaattgcctg
    21421 atccagtccg gaacagtggt aggcgcagac ggctttggtt atgccaacga tcgtggtaac
    21481 tgggtgaaga tcccacagat tggtcgcgta attattggcg atcgcgtgga gatcggtgcc
    21541 tgcacaacca tcgatcgcgg cgcgctggat gacactgtta ttggcaatgg cgtgatcatt
    21601 gataaccagt gccagattgc acataacgtc gtgattggcg acaatacggc ggttgccggt
    21661 ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact gcatgatcgg cggagccagc
    21721 gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg ttacgggcat gggtatggtg
    21781 atgcgtccca tcactgaacc aggcgtctat tcctcaggca ttccgctgca acccaacaaa
    21841 gtctggcgca aaaccgctgc actggtgatg aacattgatg acatgagcaa gcgtctgaaa
    21901 tcgcttgagc gcaaggttaa tcaacaagac taacgttcca tcttttgttc gccaaacttt
    21961 acggcctgtc tcattcttac gattgcggca ggccgtgtta ttattgtcgt ttcttatatt
    22021 ttgacaggaa gagtatcttg actactaaca ctcatactct gcagattgaa gagattttag
    22081 aacttctgcc gcaccgtttc ccgttcttac tggtggatcg cgtgctggat tttgaagaag
    22141 gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga gccattcttc cagggccatt
    22201 tccctggaaa accgattttc ccgggtgtgc tgattctgga agcaatggca caggcaacag
    22261 gtattctggc gtttaaaagc gtaggaaaac tggaaccggg tgagctgtac tacttcgctg
    22321 gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg cgatcaaatg atcatggaag
    22381 tcactttcga aaaaacgcgc cgcggcctga cccgttttaa aggggttgct ctggtcgatg
    22441 gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag ccgggaggcc tgatacgtga
    22501 ttgataaatc cgcctttgtg catccaaccg ccattgtgga agagggcgcg tcaattggcg
    22561 cgaacgcaca cattggtcct ttttgtatcg ttggacccca tgtcgaaatt ggtgagggta
    22621 ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa aattggccgc gataatgaga
    22681 tttatcagtt cgcctccatc ggcgaagtta accaggatct gaaatatgct ggcgaaccga
    22741 cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag cgtcaccatt catcgtggca
    22801 cagtccaggg cggtggattg acgaaggtgg gcagcgacaa cttactgatg atcaacgcgc
    22861 acattgcgca cgattgtacg gtaggtaacc gctgtattct cgccaacaac gcaacgctgg
    22921 cgggtcacgt atcggttgac gacttcgcga tcatcggcgg catgaccgca gtccatcagt
    22981 tctgcatcat tggtgcgcac gtgatggttg gcggctgctc cggtgtggcg caggacgtcc
    23041 ctccttatgt cattgcgcag ggtaaccacg caacgccgtt cggtgtcaat atcgaagggc
    23101 tgaagcgccg cggattcagc cgtgaggcga ttaccgctat ccgcaatgcg tataagctga
    23161 tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga aattgctgaa ctggcggaaa
    23221 catatccgga agtgaaagcc tttaccgatt tctttgcacg ctcaacgcgc ggtctgattc
    23281 gttaatgact gaacagcgtc cattaacgat tgccctggtc gccggagaaa cctccggcga
    23341 tatcctgggg gccggtttaa tccgcgctct gaaagaacat gtgcccaacg cccgctttgt
    23401 tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa gcttggtacg aaatggaaga
    23461 actggcggta atgggcattg ttgaagtgct cggtcgtctg cgtcgcttac tgcatattcg
    23521 tgccgatctg acaaagcgtt ttggcgaact gaagccagat gtttttgttg gtattgatgc
    23581 gcccgacttc aatattactc ttgaaggtaa cctcaaaaag cagggtatca aaaccattca
    23641 ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc gttttcaaaa taggcagagc
    23701 caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg ttttatgaca aatacaatgt
    23761 accgtgccgc tttatcggtc ataccatggc tgatgccatg ccgttagatc cagataaaaa
    23821 tgccgcccgt gatgtgctgg ggatccctca cgatgcccac tgcctggcgt tgctaccggg
    23881 gagccgtggt gcagaagttg aaatgcttag tgccgatttc ctgaaaacgg cccagctttt
    23941 gcgccagaca tatccggatc tcgaaatcgt ggtgccactg gtgaatgcca aacgccgcga
    24001 gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctt tcagttcatt tgctggatgg
    24061 gatgggccgt gaggcgatgg tcgccagcga tgcggcgcta ctggcgtcgg gtacggcagc
    24121 cctggagtgt atgctggcga aatgcccgat ggtggtggga tatcgcatga agccttttac
    24181 cttctggttg gcgaagcggc tggtgaaaac tgattatgtc tcgctgccaa atctgctggc
    24241 gggcagagag ttagttaaag aattattgca ggaagagtgt gagccgcaaa aactggctgc
    24301 ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac gcgatgcacg ataccttccg
    24361 tgaactgcat cagcagatcc gctgcaatgc cgatgagcag gcggcacaag ccgttctgga
    24421 gttagcacaa tgatcgaatt tgtttatccg cacacgcagc tggttgcggg tgtggatgaa
    24481 gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg cggtgatcct tgacccggcg
    24541 cgcccgattg ccgggctgaa tgattccaaa aagctgagcg aaaaacgccg tctggcgctc
    24601 tatgaagaga tcaaagagaa agcgttgagc tggagtctgg gccgcgcgga accccacgaa
    24661 atcgacgagc tgaacattct tcatgcgacc atgctggcga tgcagcgtgc cgtcgctggg
    24721 ctgcatattg cgccggaata tgtgttgatt gatggtaacc gctgcccgaa attaccgatg
    24781 cctgcgatgg ctgtggtgaa aggcgatagc cgcgtaccgg aaatcagtgc cgcgtctatc
    24841 ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg atattgtttt cccgcaatat
    24901 ggttttgccc aacacaaagg gtacccaacc gcttttcatc tggaaaaact ggctgaacac
    24961 ggcgcgaccg aacaccatcg gcgcagcttt gggcctgtca aacgcgcact gggacttgcg
    25021 tcctgattct tgtgtcgaga ttaagtaaac cggaatctga agatgtctga accacgtttc
    25081 gtacacctgc gggtgcacag cgactactcg atgatcgatg gcctggccaa aaccgcaccg
    25141 ttggtaaaaa aggcggcggc gttgggtatg ccagcactgg cgatcaccga tttcaccaac
    25201 ctttgtggtc tggtgaagtt ctacggagcg ggacatggcg cagggattaa gcctatcgtc
    25261 ggggcagatt ttaacgtcca gtgcgacctg ctgggtgatg agttaaccca cctgacggta
    25321 ctggcggcga acaataccgg ctatcagaat ctgacgttgc tgatctcaaa agcgtatcag
    25381 cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact ggcttatcga attaaacgaa
    25441 gggttgatcc ttctttccgg cggacgcatg ggcgacgtcg gacgcagtct tttgcgtggt
    25501 aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag aacacttccc ggatcgctat
    25561 tttctcgagc tgatccgcac cggcaggccg gacgaagaaa gctatctgca cgcggcggtg
    25621 gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacca acgacgtgcg ctttatcgac
    25681 agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc acgacggctt taccctcgac
    25741 gatcctaaac gcccgcgtaa ctattcgccg cagcaatata tgcgtagcga agaggagatg
    25801 tgcgagctgt ttgccgacat ccccgaagct cttgccaaca ccgttgagat cgccaaacgt
    25861 tgtaacgtaa ccgtgcgtct tggtgaatac ttcctgccgc agttcccgac cggggacatg
    25921 agcaccgaag attatctggt caagcgtgca aaagagggcc tggaagagcg tctggccttt
    25981 ttattccctg acgaggaaga acgtgttaag cgccgcccgg aatatgacga acgtctggag
    26041 actgaacttc aggttatcaa ccagatgggc ttcccgggct acttcctcat cgttatggaa
    26101 tttatccagt ggtcgaaaga taacggcgta ccggtagggc caggccgtgg ctccggtgcg
    26161 ggttcactgg tggcctacgc gctgaaaatc accgacctcg atccgctgga atttgacctg
    26221 ctgttcgaac gtttccttaa cccggaacgt gtctccatgc ctgacttcga cgttgacttc
    26281 tgtatggaga aacgcgatca ggttatcgag cacgtagcgg acatgtacgg tcgtgatgcg
    26341 gtatcgcaga tcatcacctt cggtacaatg gcggcgaaag cggtgatccg cgacgtaggc
    26401 cgcgtgctgg ggcatccgta cggctttgtc gatcgtatct cgaaactgat cccgcccgat
    26461 ccggggatga cgctggcgaa agcgtttgaa gccgagccgc agctgccgga aatctacgaa
    26521 gcggatgaag aagttaaggc gctgatcgac atggcgcgca aactggaagg ggtcacccgt
    26581 aacgccggta agcacgccgg tggggtggtt atcgcgccga ccaaaattac cgattttgcg
    26641 ccgctttact gcgatgaaga gggcaaacat ccggtcaccc agtttgataa aagcgacgtt
    26701 gaatacgccg gactggtgaa gttcgacttc cttggtttgc gtacgctcac catcatcaac
    26761 tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg gcgagccgcc gctggatatc
    26821 gctgcgatcc cgctggatga taagaaaagc ttcgacatgc tgcaacgctc ggaaaccacg
    26881 gcggtattcc agcttgaatc gcgcggcatg aaggacctga tcaagcgtct gcaacctgac
    26941 tgcttcgaag atatgatcgc actggtggca ctgttccgcc ccggcccgtt gcagtcaggg
    27001 atggtggata actttatcga ccgtaaacat ggtcgcgaag agatttccta tccggacgta
    27061 cagtggcagc atgaaagcct gaaaccggta ctggagccaa cctacggcat catcctgtat
    27121 caggaacagg tcatgcagat tgcgcaggtg ctttctggtt ataccctcgg tggcgcggat
    27181 atgctgcgtc gtgcgatggg taagaaaaag ccggaagaga tggctaagca gcgttctgta
    27241 tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac tggcgatgaa aatcttcgac
    27301 ctggtggaga aattcgcggg ttacggattt aacaaatcgc actctgcggc ctatgctttg
    27361 gtgtcatatc aaacgttatg gctgaaagcg cactatccgg cggagtttat ggcggcggta
    27421 atgaccgccg atatggacaa caccgagaag gtggtgggcc tggtggatga gtgctggcgg
    27481 atggggctga aaatcctgcc accagatata aactccggtc tttaccattt ccacgtcaac
    27541 gacgacggcg aaatcgtgta tggtattggc gccatcaaag gggtaggtga aggtccgatt
    27601 gaggccatca tcgaagcccg taataaaggc ggctacttcc gcgaactgtt tgatctctgc
    27661 gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg aaaaactgat catgtccggg
    27721 gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga actcgctggg tgatgcgtta
    27781 aaagcggcag atcaacacgc gaaagcggaa gctatcggtc aggccgatat gttcggcgtg
    27841 ctggccgaag agccggaaca aattgaacaa tcctacgcca gctgccaacc gtggccggag
    27901 caggtggtgt tagatgggga acgtgaaacg ttaggtctgt accttacggg acaccctatc
    27961 aaccagtatt taaaagagat tgagcgttat gtcggaggcg taaggctgaa agacatgcac
    28021 ccgacagaac gtggtaaagt catcacggct gcggggctcg ttgttgccgc gcgggttatg
    28081 gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg atgaccgttc cgggcggctg
    28141 gaagtgatgt tgtttactga cgccctggat aaataccagc aattgctgga aaaagaccgc
    28201 atacttatcg tcagcggaca ggtcagcttt gatgacttca gcggtgggct taaaatgacc
    28261 gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat atgctcgcgg gcttgctatc
    28321 tcgctgacgg acaggcaaat tgatgaccag cttttaaacc gactccgtca gtctctggaa
    28381 gcccaccgct ctgggacaat tccagtacat ctctactatc agagggcgga tgcacgcgcg
    28441 cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg atcgtttatt aaacgatctc
    28501 cgtggcctca ttggttcgga gcaggtggaa ctggagtttg actaatacag gaatactatg
    28561 agtctgaatt tccttgattt tgaacagccg attgcagagc tggaagcgaa aatcgattct
    28621 ctgactgcgg ttagccgtca ggatgagaaa ctggatatta acatcgatga agaagtgcat
    28681 cgtctgcgtg aaaaaagcgt agaactgaca cgtaaaatct tcgccgatct cggtgcatgg
    28741 cagattgcgc aactggcacg ccatccacag cgtccttata ccctggatta cgttcgcctg
    28801 gcatttgatg aatttgacga actggctggc gaccgcgcgt atgcagacga taaagctatc
    28861 gtcggtggta tcgcccgtct tgacggtcgt ccggtgatga tcattggtca tcaaaaaggt
    28921 cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc cagcgccaga aggttaccgc
    28981 aaagcactgc gtctgatgca aatggctgaa cgctttaaga tgcctatcat cacctttatc
    29041 gacaccccgg gggcttatcc tggcgtgggc gcagaagagc gtggtcagtc tgaagccatt
    29101 gcacgcaacc tgcgtgaaat gtctcgcctc ggcgtaccgg tagtttgtac ggttatcggt
    29161 gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg ataaagtgaa tatgctgcaa
    29221 tacagcacct attccgttat ctcgccggaa ggttgtgcgt ccattctgtg gaagagcgcc
    29281 gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg ctccgcgtct gaaagagctg
    29341 aaactgatcg actccatcat cccggaaccg ctgggcggcg ctcaccgtaa cccggaagcg
    29401 atggcggtat cgttgaaagc gcaactgctg gcggatctgg ccgatctcga tgtgttaagc
    29461 actgaagatt taaaaaatcg tcgttatcag cgcctgatga gctacggtta cgcgtaattc
    29521 gcaaaagttc tgaaaaaggg tcacttcgg
//
LOCUS       DAJVRF010000038        28716 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJVRF010000038 DAJVRF010000000
VERSION     DAJVRF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28716
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.038"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..134)
                     /gene="tuf"
                     /locus_tag="OQN13_001544"
     CDS             complement(<1..134)
                     /gene="tuf"
                     /locus_tag="OQN13_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1614931.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     R"
     gene            complement(205..2319)
                     /gene="fusA"
                     /locus_tag="OQN13_001545"
     CDS             complement(205..2319)
                     /gene="fusA"
                     /locus_tag="OQN13_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCS1614932.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="OQN13_001546"
     CDS             complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="OQN13_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCS1614933.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="OQN13_001547"
     CDS             complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="OQN13_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCS1614934.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="OQN13_001548"
     CDS             complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="OQN13_001548"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCS1614935.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3778..4137)
                     /gene="tusC"
                     /locus_tag="OQN13_001549"
     CDS             complement(3778..4137)
                     /gene="tusC"
                     /locus_tag="OQN13_001549"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCS1614936.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4137..4523)
                     /gene="tusD"
                     /locus_tag="OQN13_001550"
     CDS             complement(4137..4523)
                     /gene="tusD"
                     /locus_tag="OQN13_001550"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCS1614937.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4523..5245)
                     /locus_tag="OQN13_001551"
     CDS             complement(4523..5245)
                     /locus_tag="OQN13_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1614938.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5412..6224)
                     /gene="fkpA"
                     /locus_tag="OQN13_001552"
     CDS             complement(5412..6224)
                     /gene="fkpA"
                     /locus_tag="OQN13_001552"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1614939.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6445..6663
                     /locus_tag="OQN13_001553"
     CDS             6445..6663
                     /locus_tag="OQN13_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCS1614940.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6712..7302)
                     /gene="slyD"
                     /locus_tag="OQN13_001554"
     CDS             complement(6712..7302)
                     /gene="slyD"
                     /locus_tag="OQN13_001554"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS1614941.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7397..7597)
                     /locus_tag="OQN13_001555"
     CDS             complement(7397..7597)
                     /locus_tag="OQN13_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCS1614942.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7607..9412)
                     /gene="kefB"
                     /locus_tag="OQN13_001556"
     CDS             complement(7607..9412)
                     /gene="kefB"
                     /locus_tag="OQN13_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCS1614943.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9412..9963)
                     /gene="kefG"
                     /locus_tag="OQN13_001557"
     CDS             complement(9412..9963)
                     /gene="kefG"
                     /locus_tag="OQN13_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCS1614944.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10094..12007
                     /locus_tag="OQN13_001558"
     CDS             10094..12007
                     /locus_tag="OQN13_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1614945.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12007..13029
                     /locus_tag="OQN13_001559"
     CDS             12007..13029
                     /locus_tag="OQN13_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1614946.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13023..13241
                     /locus_tag="OQN13_001560"
     CDS             13023..13241
                     /locus_tag="OQN13_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCS1614947.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13295..14164
                     /locus_tag="OQN13_001561"
     CDS             13295..14164
                     /locus_tag="OQN13_001561"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCS1614948.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14219..14623)
                     /locus_tag="OQN13_001562"
     CDS             complement(14219..14623)
                     /locus_tag="OQN13_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCS1614949.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14925..15557
                     /gene="crp"
                     /locus_tag="OQN13_001563"
     CDS             14925..15557
                     /gene="crp"
                     /locus_tag="OQN13_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCS1614950.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15608..17698
                     /locus_tag="OQN13_001564"
     CDS             15608..17698
                     /locus_tag="OQN13_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS1614951.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17765..18985)
                     /gene="argD/dapC"
                     /locus_tag="OQN13_001565"
     CDS             complement(17765..18985)
                     /gene="argD/dapC"
                     /locus_tag="OQN13_001565"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCS1614952.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19071..19634)
                     /gene="pabA"
                     /locus_tag="OQN13_001566"
     CDS             complement(19071..19634)
                     /gene="pabA"
                     /locus_tag="OQN13_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCS1614953.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19666..20268)
                     /locus_tag="OQN13_001567"
     CDS             complement(19666..20268)
                     /locus_tag="OQN13_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCS1614954.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20258..20425)
                     /locus_tag="OQN13_001568"
     CDS             complement(20258..20425)
                     /locus_tag="OQN13_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCS1614955.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20530..21102)
                     /gene="ppiA"
                     /locus_tag="OQN13_001569"
                     /gene_synonym="rotA"
     CDS             complement(20530..21102)
                     /gene="ppiA"
                     /locus_tag="OQN13_001569"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCS1614956.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21373..22554
                     /gene="tsgA"
                     /locus_tag="OQN13_001570"
     CDS             21373..22554
                     /gene="tsgA"
                     /locus_tag="OQN13_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCS1614957.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22816..25359
                     /gene="nirB"
                     /locus_tag="OQN13_001571"
     CDS             22816..25359
                     /gene="nirB"
                     /locus_tag="OQN13_001571"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCS1614958.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25356..25682
                     /gene="nirD"
                     /locus_tag="OQN13_001572"
     CDS             25356..25682
                     /gene="nirD"
                     /locus_tag="OQN13_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCS1614959.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25808..26614
                     /gene="nirC"
                     /locus_tag="OQN13_001573"
     CDS             25808..26614
                     /gene="nirC"
                     /locus_tag="OQN13_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCS1614960.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26633..28006
                     /gene="cobA"
                     /locus_tag="OQN13_001574"
     CDS             26633..28006
                     /gene="cobA"
                     /locus_tag="OQN13_001574"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS1614961.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28253..28420
                     /locus_tag="OQN13_001575"
     CDS             28253..28420
                     /locus_tag="OQN13_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCS1614962.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         6978 a         7694 c         7197 g         6847 t
ORIGIN      
        1 cgagcagcac cgccgtaggt tttagccagt acggtggtga ttgcagcggt cagagtagtt
       61 ttaccgtggt caacgtggcc gatagtacca acgttaacgt gcggttttgt acgttcaaat
      121 ttttctttag acacggctat attccttact atagtgctct ccccttcagg agagagcacg
      181 ggactttggt attaaccctt aggcttattt accacgggct tcaattacgg cctgagcaac
      241 gttactcggc gcttcatcat acttcaggaa ttccatagtg tatgatgcac gacctttggt
      301 cagagaacgc agctgagttg cgtatccgaa catttcagac agcggtactt cagcgtggat
      361 cttaacgcca gtaacttcag attcctgacc tttgagcata ccacgacgac ggctcaagtc
      421 accgataacg tcaccggtgt tctcttccgg agtttctact tcaaccttca tgatcggctc
      481 aagcagaact ggtttcgctt tcttaaagcc ttctttaaag gcgatagaag cagccagttt
      541 aaacgccagt tcagaggagt caacgtcatg gtaagaaccg aagtgcagac gaatacccat
      601 gtctactacc gggtagcctg ccagcggacc tgctttcagc tgttcctgga tacctttatc
      661 aacggccggg atgtattcgc cagggattac accaccttta atgtcgttga tgaactcgta
      721 gcctttcggg tttgaacccg gctccagcgg gtacatgtcg ataacaacat gaccatactg
      781 accacgacca ccagactgtt tcgcgtgttt accttcaaca tcggtaactt tctggcggat
      841 agtttcacgg taagcaacct gcggtttacc tacgttcgct tcaacgttga attcacgctt
      901 catacggtca acgatgatgt cgaggtgcag ttcgcccata cccgcgatga tggtctggtt
      961 agattcttcg tcagtccata cacggaaaga cgggtcttct ttagccagac ggcccagagc
     1021 cagacccatt ttttcctggt cagctttggt tttcggttca actgcgatgg agattaccgg
     1081 ctcagggaat tccatacgtt ccagaatgat cggcgcatcc gggtcacaca gggtgtcacc
     1141 agtggttacg tctttcagac cgatagcagc agcgatgtcg cccgcgcgaa cttctttgat
     1201 ctcttcacgt ttgttagcgt gcatctgaac gatacgaccg aaacgctcac gtgcagcttt
     1261 cacggagttc agtacggtat caccagagtt aaccacaccg gagtaaacac ggaagaaggt
     1321 caggttacca acaaacgggt cggtagcgat tttgaacgcc agtgcagaga acggctcgtc
     1381 atcacttgcg tgacgctcag ccggagtgtc tttaccgtcg tccaggatac cgttgatcgc
     1441 aggtacgtca accggggatg gcaggtaatc aattaccgca tccagcatcg cctgaacacc
     1501 tttgttcttg aacgcagaac cacaggttac caggatgatt tcgttgttca gaacgcgctg
     1561 acgcagagca cctttgattt ctgcttcagt cagttcttca ccacccaggt atttttccat
     1621 cagctcttca gaagcttcag ctgcggattc gatcaggttc tggtgccatt cgttagccag
     1681 ttcaaccatg tctgccggga tatcttcgta ttcgaaggtt acgccctggt cagcgtcgtt
     1741 ccagttgata gctttcattt tcaccaggtc aacaacaccg gtgaaatgtt cttcagcacc
     1801 aatcgccagc tgcagcggaa ccgggttcgc gcccagacgg gttttgatct ggttaacaac
     1861 tttcaggaag ttcgcaccca tgcggtccat tttgttaacg aacgcaatgc gcggaacttt
     1921 atatttgttt gcctgacgcc atacggtttc agactgcggc tgaacaccac caactgcgca
     1981 gtaaaccatt accgcaccat cgagaacacg catggaacgt tctacttcga ttgtgaagtc
     2041 aacgtgcccc ggggtgtcga tgatgttgat gcgatgcggc tcatactgct tagccatacc
     2101 agaccagaat gcagtagtcg cagcggaagt gatggtaata ccacgttcct gctcctgctc
     2161 catccagtcc atggttgcag cgccgtcatg aacttcaccg attttatggt ttacaccggt
     2221 gtagaacaga atacgttcgg tagtagtggt tttaccggcg tcgatgtgcg cactgatacc
     2281 gatgttacgg tagcgtgcga tgggtgttgt acgagccatt tgtttcctcg tttatctttt
     2341 aggcgttcaa tttaagtagc ccaaagcggg ctgcttactg gaagcgcccg cctggtgact
     2401 aaaactccga agggattacc aacggtagtg tgcgaacgcc ttgttggctt cggccatacg
     2461 gtgaacgtct tcacgtttct taactgcagt acctttgttt tctgcagcat cagaaagttc
     2521 gttcgccagg cgcagagcca tggatttatc accgcgttta cgagcagctt caacgatcca
     2581 acgcattgcc agagcattac gacgaaccgg acggacttca actggtacct gataagtaga
     2641 accaccaacg cggcgagact taacttctac agtcgggcgc acgttttcga gagctacttc
     2701 gaatgcttcc agttcagatt taccagagcg ctgagccagg gtctccagcg cgctgtatac
     2761 gatagattca gcagtagatt ttttaccatc taccatcagg atatttacaa atttagccag
     2821 cagttctgat ccaaacttcg gatccggcag aattttacgc tgaccaatga cgcgacgacg
     2881 tggcatggaa atactccgtt gttaattcag gattgtccaa aactctacga gtttagtttg
     2941 acatttaagt taaaacgttt ggccttactt aacggagaac cattaagcct taggacgctt
     3001 cacgccatac ttggaacgag cctgcttacg gtctttaacg ccggagcagt caagcgcacc
     3061 acgtacggtg tggtaacgaa cacccgggag gtctttaaca cgaccgccac ggatcaggat
     3121 cacggagtgc tcctgcaggt tgtgaccttc accaccgatg taggaagtca cttcgaaacc
     3181 gttagtcaga cgaacacggc atactttacg cagcgcggag ttcggttttt taggagtggt
     3241 agtatataca cgagtacata cgccacgttt ttgcgggcat gcttccagcg caggcacgtt
     3301 gcttttcgca actttgcgag cacgtggttt gcgtaccagc tggttaactg ttgccattaa
     3361 atagctcctg gttttagctt ttgcttcgta aacacgtaat aaaacgtcct cacacaatat
     3421 gaggacgccg aattttaggg cgatgccgaa aagatgtcaa gaaatataca acgatcccgc
     3481 catcaccagg ccatctggct ggagtgctta accgtaagtc tgacgaaatc agtatagtca
     3541 atgggaatga tgtcgttcga aatttgacca gtcaaaccgc gggcaataag gtcttcgttc
     3601 agggcataga ccttaatggg ggcattacgc agactttcaa ggtagcggtt accgtcaact
     3661 gcggcagtta cgccatcttg caataatagc agttcgtcgc cttcactgag cagacgcagc
     3721 agcgcagcaa aatccgccag ccagggtgaa cgatgtaatg tgtgcagcat aaaagcctca
     3781 aaacctcaaa ataacatcgt agttggcgag ttcgcggcgt aaggcatctg cttcgagcgg
     3841 cgtggcttcg acaacaaagg gggtctgcgg atctaacccg cgttcgcgca gtgaagccgc
     3901 acaaacccag cactgttcaa tgtcgtacag acccaacaat ttaaaagtgg caatgtaatc
     3961 acgcgccagc actgcatcgg gcttttgtcc tggcagcagc tgaaaaacgc catcagctat
     4021 aaagaagaca gccagatcat cagttaatgc ggaagttgcc agtaaagcat ctaaaccttc
     4081 ccgaccagca gctgtaccat gaggcgcagt agaaaaaaca aacgcaattc gtttcatcag
     4141 aactgtacca ccctgtcaca ggtcagcgag gcttccgcca gcgcgccaag tccgcttaag
     4201 gtaaatcccg gctgaaggtt tgacgaggcc agccccagcc ttccggcctc cgtttcatca
     4261 acaacgccac ggcgtaatgc tgccgctacg cagatattca gcgccacacc atgttgcgca
     4321 ttcaactgtt gccagccgcg cacaaggtca aattcgtcgc ttgccggtga ggtcaattgg
     4381 ttagcgttat agaccccttc ccgataaaaa aagacgctgc ttaactcatg gccttctact
     4441 atcagcgcct gcgcaaactg aaaagcacta ctcgcctgtt gcgtaccgta tgctggcccg
     4501 gtcaccacga tggcaaaacg cattacttat cttgcccctg gaaatcaccg cttttgaact
     4561 ggcggatgta gagatagaca gtgtgtttgg agatgttcag gcggtcagca acctggttga
     4621 tcgcgtcttt aatatcgaag atccctttct cgtagagatt cagcacgatc tgacgatttt
     4681 tggcgttatt agaaacattg cgatcggcat ttacttcttc gatggtgaac tccagcgttt
     4741 gggtaaccag atcttcaaca gaagaggcaa agttgacgct tgaaccgaca tccggggttt
     4801 ctggcggcac aaaagtgctc ataatctgcg agaagggaac atcaagattc atattgatgc
     4861 acagcagacc aattacacgc tgttcgcggt tacgaatcgc gatagtcagg gacttcatta
     4921 atacgccgct tttggcgcga gtaaagtagc atttagaaac gctgctatcc gctcccgtca
     4981 tatcgtgcag catacgtagc gccaggtcag taattggcga accaatcttc cggcctgtat
     5041 gttcaccgtt agcaatgcga atggctgaac attttagatc ctgcaaagag tgcaaaacga
     5101 tttcacagtg ggagccaata agcatcgcta acccgtccac caccgcttcg taggatttca
     5161 gaatatcaaa atcggtctgg tcgaaaggac gttgatccag taaatccaac tcactggttt
     5221 cgttggttaa aagcgacctg gacatgaaaa aaaacactcc ttttcaggag cctgtcgtta
     5281 acttttcagg gcaggctcat taatgatgcg ggtaactaaa ttaatacagc ggaggttccg
     5341 ctttccagca ctaattatat ccggcctgta ataaaaaaac cgccgcctgg tcaggcggcg
     5401 gttcttaatg cttatttttt agcagaatct gcggctttcg catcagcttc cggctttgca
     5461 tcagccttcg gcgctggttt cacatccagc agctctacgt caaacaccag ggtagaattc
     5521 ggtgggatcc ccggaacacc cgctttgccg taagccagtt ctggtggaat aaccagtttg
     5581 atcttaccgc ctttcttgat gttcttcaga ccttctgtcc accccgggat aacaccgtcc
     5641 agacggaaag agagcggttc accacgggtg taagagttgt cgaactcttt accgtcgatc
     5701 agcgtacctt tgtagttcac tacaacagta tcgctgtctt tcggtgcttc gcctttaccg
     5761 gcttctacta cctgataaac cagaccagtt gaagaggttt tcacaccttt ctctttggca
     5821 aatttctcgc ggtactcttt accttttgct tcgttatcag ccgcgtcttt ttccatcttc
     5881 gcctgagcag aagacttcac gcgagcttcg aatgcttgca gagtctgttc gatctcttgg
     5941 tcggagagtt tgctcttatc agcaaatgca tcctgaacac cagcgatcag ctgatcttta
     6001 tccagtttga tgcccagttt ttcttgttct ttaagagagt tttccatgta acgacccagc
     6061 gaagcaccca gtgcataagc tgatttctga tcgtcatttt tgaacgctgc tttgctgtca
     6121 gcagttgtag caggttttgc agcttcagca gcaaaagtga ttggtgcatg cagggcaacg
     6181 gccattgtgg tcgccagcag cgttacttta aacagtgatt tcatccatat ctccaggatc
     6241 ggggcatctc accccagggt taactattat cagaagggta ctataaagcg ttgtcgaaca
     6301 aatctacata cagacacgcc ctattatcat ctattttcag actctttttg tttaaattag
     6361 tttcgatgac cgcgaaatga gtgctgtctc gggcagcaaa gttaagtaga atccgcggcg
     6421 accattcgac aaaagaggtg aatcatgcag gatttatcat tggaagcacg cctggcagag
     6481 ctggagagcc gactggcttt tcaggaaatc accattgaag aactgaacgt cacggtgacc
     6541 gctcatgaaa tggagatggc gaaactgcgc gatcatctgc gtctgctgac cgagaagtta
     6601 aaagccagcc agccgtcgaa catcgcgtcg caggctgaag aaacgccacc gccacattat
     6661 tgaggcgtaa aaaaagcggg gattccccgc ttttttgtca ccttttcggt attagtggca
     6721 accacaaccg ccgttgcctt taccgccaca gcagccttcg ccaccgtgtt catgaccgtg
     6781 atcgtggcca tgaccgccgc agcaaccgtc gtggtcgtga tcgtggtgat gatcgtgcgc
     6841 gccgtgaacg tgaccatgag ccagttcttc ttcggtcgct tcacgaatcg ccacaacctc
     6901 aacgttgaat ttcaggttct gaccggccag catgtggtta ccatcaacca ctacgtgatc
     6961 gtcttcaacc gcagtgattt caaccggtac cggaccctgg tcggtttcag ccaggaaacg
     7021 catacctacc tgcagttcat caacgcccat aaatacgtct ttaggaacac gttgcaccag
     7081 gttttcgtcg tactgaccgt aagcgtcgtt cgcgccaaca gcgacatcaa atttgtcgcc
     7141 aacttcatga ccttccagcg ccgtttccag gccagagatc agggaaccgt gaccatgcag
     7201 gtagtccagc ggcgcactca ccggagactc atcaaccaac acaccgtctt ctgtacgtac
     7261 ctgataggcc aggctgacca ccaggtcttt tgctactttc atgatatctc ctgagcatgg
     7321 gaagaatagt ggcgcagatt gtagcggaat tctgcagccg tgtactcact agcttaaaaa
     7381 aactcggcgc atatcgctag tccggatgaa aaatcccgat cacttgctca tctttgcgaa
     7441 cgtgatcgcg ggcttctttg tctgcttctc gcatctgatg tccgcactta acgcattcaa
     7501 caatatcaat attattttcg cgccacatcg ccattgaatc ctgtgcctga caggccgggc
     7561 attttgcgcc cgcaataaaa cgttttcgga ttgccatctt taccctctac tcaaattcat
     7621 cccagccgtc cagctggcgt cgttcttgtt gcatttcacg ctggaaaatc tcttccagtt
     7681 cgcgtcgggc ttccctggcg cgagaaattt gtacagtatc ggcatgcatt gggatgagct
     7741 ctcgcagcat ccgcatatcc aggcggcgaa aatgcagttg cgcccgctgc gcctgatgcg
     7801 gatgcatccc aagcgtgacc aaagtcttgc gccccagctc taacgcactg gagaatgttt
     7861 cacgggaaaa ctgcgtcacc cctgcctgta ataactcatg cgcttccaca cgtccgcgcg
     7921 ctcgcgcaag aatatgcaaa tgcggaaagt gctgctggca tatttccacc agcttcatgg
     7981 tgtcttccgg ctcgttacag gtaatgacga tagactcagc ggcctctgca cccgcagaac
     8041 gtaatagatc gacctgcgtg gcgtcgccgt aataaacttt gtagccatat ttacgcatca
     8101 ggttaacggc gctgatatcc cgctccagca cggtaatgcg cattttattt gccatcagca
     8161 aacgaccaat cacctgacca aaacgcccga agcccacgac aatgacctgg ggtttatcat
     8221 cgttgaccca cggtttttcg tcttcttctt ccggtccgtt aaactggcgg gatagccatt
     8281 tatccaccag cttcatcagc aacggcgtgg tcatcatgga aagcgtcacc gtcaccagca
     8341 acagcgccat ctggtcgccc tggaataaac gttgtgaaga agcggtagaa aagaggacaa
     8401 aggcaaactc accgccctga ctcaacacgc cagcaaactg catccgctct gagctacgca
     8461 cgccatacaa tcgcgccagc agatacagca cgagaatttt caccgccacc agcacaacca
     8521 cgcttatcac tacccacaac agatgggtat aaagcacccc gaggttgagc gacatgccga
     8581 cagagataaa gaacaaaccg agcagcaagc ctttgaaggg atcgatagcc gtttccagtt
     8641 catggcgata ttcactttcc gccagcagca cgcccgcaat aaatgtaccg agcgccatcg
     8701 acagccccag cgcatccata aacaatgcgg aacccaacac cagcagcagc gtcgcggcgg
     8761 tgaacacttc ccgcacgcca gaagctgcaa taaagcggaa taccggacgc agtaaatagc
     8821 gcccaccaat cagcatgccg acaaacgcca gcaccttcat gccgatcttc atccagtcga
     8881 aatgttcgtc tgccgacccc gccaacaacg gcactaacgc cagtgctggg atcactgcca
     8941 gatcctgaaa cagcagaacc gaaaacccga gctggccgga ttcgctgcga ttcatccctt
     9001 tctcacgcat caattgcaac gccattgcag ttgaagacat cgcaaggcca atgccaccga
     9061 ccaccgccgc ctgccaggcg aaatccgtca gcatcaataa tcccgccagc aacgccgcgc
     9121 ttaacagcac ctgtgccgcg cctacgccaa aaatcgaacg ccgcagttgc caaagtttgg
     9181 agggattcaa ctcaaggccg atgataaaca tcaggaatac cacgccgagt tccgaaaagt
     9241 ggaggatctc atcgacgtcg ctaataaacc ccagccccca cgggccaatt gcaatccctg
     9301 ccagcaaata tcccaacaca gcgccaatcc ccagccgcga tgccaacggc accgcagcca
     9361 ccgccgcgaa gagaaacagc actcctgcga gtaaaaaatc ggaaccttcc atcagcggcc
     9421 tcctggagac agcggatttg ccagccagtc accgtaggct ctggcgtggc tcgccagctc
     9481 ctttgcgctt tgccgtctcg cccagtaaat aatgatggga cttaaccaat gcatccggca
     9541 catgcccgcc gccagttcaa aggggcgcag cacatcgctc atcgggtagc gattcagcgc
     9601 gtcataacgg taagcacttt ccggctcgcc ggtggtaatc acgttacgcc agtactttcc
     9661 cgccagttgg tttcctcccg gcccgctggc aaaaccacga cttaataccc ggtccagcca
     9721 ctctttcagt agcgccgggc agctataggt ataaagagga tgctgaaaga caatcacctc
     9781 gtgctcgcgc agtaatgcct gctcacgggg gatatcaata aaaaaatcgg gatagtgcgc
     9841 gtaaaggtcg tgcacggtaa cattgctgag ctgcgtggcc ggtttaagca gtacccggtt
     9901 tgccaccgag tcctgagatt ccggatgggc atacagcagc aaaactttcg ctggctgaga
     9961 catcatcccc ctcccggtat ggtttttgtg tatagtcgct gttttgggct accattgcgc
    10021 ccggtgcggc agctcgccca tacattacat tatcataatg ataagttaac atagtctgaa
    10081 catacggcgc cttatgattg ttttctcctc gttacaaatt cgtcgcggcg tgcgcgtcct
    10141 gctggataat gccaccgcca ccatcaaccc cgggcagaaa gtcggcctgg tgggtaaaaa
    10201 cggctgtggt aaatctaccc tgctggcatt gctgaaaaat gaaatcagcg ccgacggcgg
    10261 cagctacacc tttccgggaa gctggcaact ggcgtgggtg aatcaggaaa cgccagcgtt
    10321 accgcaagcg gcgctggaat atgtcattga cggcgaccgt gaatatcgtc aactggaagc
    10381 gcagctgcac gacgccaacg aacgtaacga cgggcacgcc attgcgacca ttcatggcaa
    10441 gctggatgct attgacgcat ggagtattcg ctcccgtgcc gccagcctgc tgcacggcct
    10501 cggtttcagc aatgaacaac tggagcgccc ggtaagtgat ttctccggtg gctggcgtat
    10561 gcgtcttaac cttgcccagg cgctgatttg ccgttcagac ttgctgctgc tcgacgaacc
    10621 gactaaccac ctcgatctcg atgccgttat ctggctggaa aaatggttga agagctatca
    10681 gggcacgctg atcctgatct ctcacgaccg cgacttcctc gatccgatcg ttgataaaat
    10741 tattcatatc gaacaacaaa gcatgttcga gtacaccggc aactacagtt cgtttgaagt
    10801 acagcgcgcc acccgtctgg cgcagcaaca agcgatgtat gaaagccagc aggaacgcgt
    10861 ggcacatctg caaagttata tcgaccgttt ccgtgccaaa gccaccaaag cgaagcaggc
    10921 ccagagccgc attaagatgc ttgagcgtat ggagctgatt gcccccgcgc acgtcgacaa
    10981 cccgttccgc tttagcttcc gcgcgccgga aagcctgcca aatccgttac tgaagatgga
    11041 aaaagtcagc gcgggctatg gcgatcgcat tattctcgac tcgattaaac tgaacctggt
    11101 gcccggctcg cgcattggtc tgttaggccg caacggcgcg ggtaaatcga cattaatcaa
    11161 actgttagcc ggtgaacttg cgccagtcag cggtgaaatt ggtctggcga aagggatcaa
    11221 gctcggctac ttcgcccagc atcaacttga atacctgcgc gccgacgaat cacctattca
    11281 acatctggca cgtttagcgc cgcaggagct ggaacaaaaa ctgcgtgact acctcggcgg
    11341 ctttggtttc cagggcgata aagtaaccga agaaacgcgc cgcttctccg gtggggaaaa
    11401 agcccgcctg gtgctggcat taattgtctg gcagcgtccg aatctgctgc tgctcgacga
    11461 accgactaac caccttgacc tcgacatgcg tcaggcactc accgaagcat taatcgagtt
    11521 tgaaggcgcg ctggttgtcg tttcgcacga ccgtcatttg ctgcgttcca ccactgacga
    11581 tctctacctg gttcacgatc gtaaagtcga accgttcgac ggcgatctgg aagattatca
    11641 acagtggttg agcgacgtac aaaagcagga aaaccagacc gacgaagcgc caaaagagaa
    11701 cgcgaacagc gcccaggcac gtaaagatca gaagcgtcgg gaagcggagc tgcgtgcgca
    11761 aacccagccg ctgcgtaaag agattgcccg tctggaaaaa gagatggaga agctgaacgc
    11821 gcaactggcg caggcggaag agaaactcgg agacagcgaa ctgtatgacc agagccgtaa
    11881 agcggagttg accgcctgcc tgcaacagca agccagcgcc aaatccggcc tggaagagtg
    11941 cgaaatggcg tggctggaag cccaggagca gcttgagcag atgctgctgg aaggccaaag
    12001 caactgatgg cgcagataac gacgaccgat gccaatgaat tcagcagcag tgctgaattc
    12061 acccctatgc gcggctttag caattgtcat ctacaaacta tgctgccgcg tttgtttcgt
    12121 cgtcaggtga aattcacccc gtactggcag cggctggagt tgcccgacgg cgattttgtc
    12181 gatctcgcgt ggagtgaagc ccctgcacag gctcggcata aaccgcgttt agtggtattt
    12241 cacgggctgg aaggcagtct caatagccct tacgcccacg gtctggttga ggcggcgcaa
    12301 aagcgcggct ggctgggcgt ggtgatgcat tttcgcggat gcagcggtga accaaaccgt
    12361 atgcaccgca tttaccattc gggcgaaacc gaagacgcca gttggttttt acgctggctg
    12421 caacgcgaat ttggtcatgc gccaacggct gccgtcggct attcgctcgg cggtaatatg
    12481 ctggcctgtt tgctggcaaa ggaaggcaat gatctcccgg ttgatgcggc ggtgattgtc
    12541 tctgcgccgt ttatgctgga agcctgtagc tatcatatgg aaaagggctt ttcccgcgtt
    12601 tatcagcgtt acttgctgaa cctgttaaaa gccaatgccg cgcgcaagct ggcagcctac
    12661 cccggaacgc tgccgattaa tctcgcgcag ttaaaatcgg tacgtcgcat ccgtgaattt
    12721 gacgatctaa tcaccgccag aattcacggc tacgccgacg ctatcgacta ttatcgtcag
    12781 tgtagcgcca tgccgatgct gaaccggatc gccaaaccga cgctgattat tcacgccaaa
    12841 gacgatccgt ttatggatca tcaggtgatc ccgaaaccgg aaagtctccc cccgcaggtg
    12901 gagtatcaac tgactgaaca tggcggtcat gttggcttta ttggcggtac attacttcat
    12961 ccgcaaatgt ggctggagtc acgcattcct gactggttaa caacgtatct ggaggcgaaa
    13021 tcatgttgat tccgtggcaa gacctctccc ccgaaacgct ggaaaattta attgaaagct
    13081 ttgtgttacg tgaaggcacc gattatggtg aacatgagcg tacacttgaa cagaaagtcg
    13141 ccgacgtcaa acgccagcta cagtgcggag aagcggtgct ggtatggtcg gagctgcacg
    13201 aaacggtcaa tatcatgccg cgcagtcagt ttcgcgaata gcctgcaacc gtcggcggac
    13261 gagataataa tgatagctaa gtcatggagt tactatgtct gccaaacatc cggtcattgc
    13321 ggtaacagga tccagcggcg cggggaccac caccaccagc ctcgcgtttc gtaaaatatt
    13381 cgcgcagtta aatctgcatg cagctgaggt ggaaggcgac agttttcacc gttacacccg
    13441 cccggaaatg gacatggcga tccgcaaagc gcgcgacgcc gggcggcata tcagctactt
    13501 cggccccgag gctaacgact tcggcctgct ggaacaaacc ttcattgaat acggtcagag
    13561 cggcaaaggg aaatctcgca aatatctgca tacctacgac gaagccgtac cgtggaatca
    13621 ggtaccgggg acattcaccc cctggcaacc tttaccggaa cccactgatg tactgtttta
    13681 tgaaggttta cacggcggcg tagtcacgcc acagcataac gttgcgcagc atgtggactt
    13741 actggtcggc gtggtgccta tcgttaacct tgagtggatt caaaaactga tccgcgacac
    13801 cagcgagcgc gggcactcac gagaagcagt gatggactca gtagtgcgtt caatggaaga
    13861 ctatatcaac tacatcacac cgcagttttc ccgcacccat cttaacttcc agcgcgttcc
    13921 caccgtcgac acttcaaacc cgttcgcggc aaaaggtatc ccgtcgctcg atgaaagctt
    13981 tgtggtgatc cattttcgta atctggaagg gatcgatttc ccctggctgc tggcgatgtt
    14041 gcaaggctca ttcatttccc acatcaatac gttagtggta ccgggcggca aaatgggtct
    14101 ggcaatggaa ttaattatgc tgccgctggt gcaacgattg atggaaggaa agaaaatcga
    14161 gtaactctgc tattacgccg gataaaatac tatccggctt cacaacggga tagttaaatc
    14221 acgcggcaac cacttcatac gagtgagtaa tattcaccgc tttttccagc atcaacgcca
    14281 ctgaacaata tttctcagca gagagatcaa ccgcacgcgc aaccgctgcg tctttcaggt
    14341 cgcgaccggt gacgataaaa tgcagattaa tgtgcgtaaa caggcgtggt gcctcttcgc
    14401 ggcgttcaga ggtcaatttt acttcacaat cgaccacatc ctgacgccct ttttgcagga
    14461 tagaaaccac atcgatggca ctgcaaccac ccgccgccat caacaccatt tccatcggac
    14521 ttggtgcttt atcgcctgag ttgccgtcca ttaaaatctg gtgaccagag gcggattcgc
    14581 ccagaaaagt taacccttcg acccacttca ctcgcgcttg catttttgct actccactgc
    14641 gtcaattttc ctgacagagt acgcgtacta accaaatcgc gcaacggaag gcaacctggg
    14701 tcatgctgaa gcgagacacc aggagacaca aagcgaaagc tatgctaaaa cagtcaggat
    14761 gctacagtaa tacattgatg tactgcatgt atgcaaagga cgtcacatta ccgtgcagta
    14821 cagttgatag ccccttccca ggtagcggga agcatatttc ggcaatccag agacagcggc
    14881 gttatctggc tctggagaaa gcttataaca gaggataacc gcgcatggtg cttggcaaac
    14941 cgcaaacaga cccgactctc gaatggttct tgtctcattg ccacattcat aagtacccat
    15001 ccaagagcac gcttattcac cagggtgaaa aagcggaaac gctgtactac atcgttaaag
    15061 gctctgtggc agtgctgatc aaagacgaag agggtaaaga aatgatcctc tcctatctga
    15121 atcagggtga ttttattggc gaactgggcc tgtttgaaga gggccaggaa cgtagcgcat
    15181 gggtacgtgc gaaaaccgcc tgtgaagtgg ctgaaatttc gtacaaaaaa tttcgccaat
    15241 tgattcaggt aaacccggac attctgatgc gtctgtctgc acagatggcg cgtcgtctgc
    15301 aagtcacttc agagaaagtg ggcaacctgg cgttcctcga cgtgacgggc cgcattgcac
    15361 agactctgct gaatctggca aaacaaccag acgctatgac tcacccggac ggtatgcaaa
    15421 tcaaaattac ccgtcaggaa atcggtcaga ttgtcggctg ttctcgtgaa accgtgggac
    15481 gcattctgaa gatgctggaa gatcagaacc tgatctccgc acacggtaaa accatcgtcg
    15541 tttacggcac tcgttaatcc cgtcggagtg gcgcgttacc tggtagcgcg ccattttgtt
    15601 tcccccgatg tggcgcagac tgatttatca ccccgatatc aactatgcac ttcgacaaac
    15661 gctggtgcta tgtttgcccg tggccgttgg gttaatgctt ggcgaattac gattcggtct
    15721 gctcttctcc ctcgttcctg cctgttgcaa tattgcgggc cttgatacgc ctcataaacg
    15781 ttttttcaaa cgcttaatca ttggtgcgtc gctgtttgcc acctgtagct tgctgacaca
    15841 gctactactg gcaaaagatg ttcccctgcc ctttttgctg accggattaa cgctggtact
    15901 tggcgtcact gctgagctgg ggccattgca cgcaaaattg cttcctgcat cgctgctcgc
    15961 cgccattttt accctcagtc tggcgggata catgccggtc tgggaaccgt tgctcatcta
    16021 tgcgttgggc actctctggt acggattgtt taactggttt tggttctgga tctggcgcga
    16081 acaaccgctg cgcgagtcac taagtctgct gtaccgcgaa ctggcagatt attgtgaagc
    16141 caaatacagc ctgcttaccc agcacaccga ccctgaaaaa gcgctgccgc cgctgctggt
    16201 gcgccagcaa aaagcggtcg atctaattac ccagtgctat cagcaaatgc atatgctttc
    16261 cgcgcaaaat aatactgatt acaagcggat gctgcgtatt ttccaggagg cgctggattt
    16321 acaggaacat atttcggtca gtttgcatca gccggaagag gtgcaaaagc tggtcgagcg
    16381 tagccatgcg gaagaagtta tccgctggaa tgcgcaaacc gtcgccgctc gcctgcgcgt
    16441 gctggctgat gacattcttt accatcgcct gccaacgcgt tttacgatgg aaaagcaaat
    16501 tggcgcactg gaaaaaatcg cccgccagca tccggataat ccggttgggc aattctgcta
    16561 ctggcatttc agccgcatcg cccgcgtgct gcgcacccaa aaaccgctct atgcccgtga
    16621 cttactggcc gataaacagc ggcgaatgcc gttacttccg gcgctgaaaa gttatctgtc
    16681 actaaagtct ccggcgctac gcaatgccgg acgactcagt gtgatgttaa gcgttgccag
    16741 cctgatgggc accgcgctgc atctgccgaa gtcgtactgg atcctgatga cggtattgct
    16801 ggtgacacaa aatggctatg gcgcaacccg tctgaggatt gtgaatcgct ccgtgggaac
    16861 cgtggtcggg ttaatcattg cgggcgtggc gctgcacttt aaaattcccg aaggttacac
    16921 cctgacgttg atgctgatta ccaccctcgc cagctacctg atattgcgca aaaactacgg
    16981 ctgggcgacg gtcggtttta ctattaccgc agtgtatacc ctgcaactat tgtggttgaa
    17041 cggcgagcaa tacatccttc cgcgtcttat cgataccatt attggttgtt taattgcttt
    17101 cggcggtact gtctggctgt ggccgcagtg gcagagcggg ttattgcgta aaaacgccca
    17161 tgacgcttta gaagcctatc aggaagcgat tcgcttgatt cttagcgagg atccgcaacc
    17221 tacgccactg gcctggcagc gaatgcgggt aaatcaggca cataacactc tgtataactc
    17281 attgaatcag gcgatgcagg aaccggcgtt taacagccat tatctggcag atatgaaact
    17341 gtgggtaacg cacagccagt ttattgttga gcatattaat gccatgacca cgctggcgcg
    17401 ggaacaccgg gcattgccac ctgaactggc acaagagtat ttacagtctt gtgaaatcgc
    17461 cattcagcgt tgtcagcagc gactggaata tgacgaaccg gggagttctg gcgatgccaa
    17521 tatcatggat tcgccggaga tgcagccgca cgaaggcgcg gcaggtacgc tggagcagca
    17581 tttacagcgg gttattggtc atctgaacac catgcacacc atttcgtcga tggcatggcg
    17641 tcagcgaccg catcacggga tttggctgag tcgcaagttg cgggattcga aggcgtaatg
    17701 caggctaaac catcggataa ggcattcacg ccgcatccga catcttttgc ctgatgcttc
    17761 gtatttacgc cccaaccacc ttcgccaccg cgtgggcgaa acgttgcatc ccttcatcga
    17821 tatccgcatc ttccaccacc agcgacggtg caaaacgcat cacatccggt ccggcattca
    17881 gcaccattac gccagcctct gcgcccgcat acaggaaatc acgcgcccga cctttgtact
    17941 gtggtttcag ctctgcgcca atcaacagcc ccataccgcg aatatcgcta aacacatcgt
    18001 actgctgatc gatcttctgc agatggtcaa caaaacgctg gcgtttcgcc tgaatgcctt
    18061 ccagcacttc agggggattg atgatatcaa acgccgcccc cgctactgca caggccagag
    18121 gattaccgcc gtaggtggaa ccgtgagaac caggatgaaa cgcagaagca atttccgccg
    18181 tggtcagcat ggcgctaatc gggaagccgc cgcctaacgc tttcgcagag gtcagaatat
    18241 ccggcgtcac gccgtagtgc atgtaagcaa acaaatcgcc ggtccgcccc atcccgcact
    18301 gcacttcatc aaacaccaat aatgcctgat gttgatcgca cagctcgcgc aagccctgca
    18361 aaaactctgg cgtcgcagcc gtcacgccgc cctcgccctg gatcggctca accaccaccg
    18421 cacaggtgtg atcatccatc actgctttca ctgcgtggag atcgttaaag ggaacgtgga
    18481 tgatgtctgc cggtttcggc ccaaagccgt cggaatattt tggctgccca cccaccgaaa
    18541 cggtaaacag cgagcgacca tgaaaagcgt tatggaaggc aataattttg gttttgaacg
    18601 ggctatgacg cacacaggcg taatgacgtg ccagtttaaa ggcggtttcg ttagcttccg
    18661 tgccggagtt cataaacacc acgcgttcgg caaacgttgc ctcaatcagt ttacgcccaa
    18721 gacgcagcgc cggttcattg gtgaaaacgt tactgatatg ccacagaatt tcgccctggg
    18781 tttttaacgc gttcaccagc gcaggatggc aatggcccaa cgccgtaact gcaatgccac
    18841 ccgcgaaatc gacatactcc ttgccttgct gatcccagat tcggctgccc tgacctttta
    18901 ccggaataaa ctctgccgga gcataaatcg gcaggatcac ttcatcgaaa gtcgcgcgtg
    18961 taattgctgt ttgttcaatt gccatctcat gatcaccctg ttacgcataa acaaatgtga
    19021 aattataatc acaaaatatg cataaaaaat cactaaatgg caatcagaaa tcagcgatgc
    19081 aggaaattag ccagcagttg atgtccttgt tcgctaagaa tactttctgg atggaactgc
    19141 acaccttcca gatcccactg gcgatggcga atccccataa tctcgcgggt ttcgctccag
    19201 gccgtcacgt caaagcacgc tggtaatgag tcaggttcca ccaccagcga atggtagcgt
    19261 gtcacggtaa gtggatttgc cagcccccga aatacgccct caccgctatg tgtaatcggc
    19321 gaggttttgc cgtgcatgac ctttgcggcg cgcacaactt tgccgccaaa tgcctgcgcc
    19381 attgcctgat gaccgaggca gacgccaaga atcggcaagc gcccggcata gtgacgaata
    19441 acgtcaaggg agatcccggc ttcatctggc gtacaggggc caggtgagat gacaattttt
    19501 tgtggtttaa gggcgtcgat atccgccagc gtcaacgcat cgttgcgctt aaccagcaca
    19561 tccgccccca gttcacaaaa gtactggtag aggttccagg taaaagaatc gtagttatct
    19621 ataagcagga tcatggcggc tccggtacga aagaaccgcg ctattttact cagattcccc
    19681 ggctttgctt accactttgc taaatatcat ctgcagtgcg gtgagatccc ccattgcccc
    19741 actctgattt gcctgattcc aggcctcttt ttcgataccc tgccagctca gttgatatcc
    19801 ggcatgaatc gccagttgct cgaagaagat ccgctgtgcc agaccacttc ccacccggaa
    19861 gggatgcagc acgttgattt cacaatagta atgcgccagc cgctcgacga acttcgcttt
    19921 ctccaggcca accagataac cttcttcctc cagatcctgc atcagggcat tgccctcttt
    19981 ttcgatataa gcaaagtggc agaacggcgt atcgccctga taaatatcaa cttcacgcag
    20041 ttgccctgcc cagtcgaaaa tatcctggta cagctggcga tggatagtgc gcaaatgcgg
    20101 taaaccgcgc accagcggac caagctcaat ggtcgcagca cgcagcgccg tcatttcgta
    20161 agcggcctgt tccagccgct gctgctgacg gatgttcagc cggttacgca tgatatcaag
    20221 gccaggataa agatacggat cgcgcccttc accgaattta tcgctcatag tgcctcctca
    20281 gctcttcaag gcgcgctaaa gcctctgcgg cagtaagagt gactaaaggc atctcgacgc
    20341 cttcaagacg gcgactggcc tggaaattac gattacgctg aagctcccag agacgggact
    20401 tttgcttatc ggtgagtttc ttcacttgat gcctccctga acgtgtccat ttgccacaag
    20461 tataagcggc aaatccgggt tacgccggga gagcagaagc gcgggcaaga ttgcccgcgc
    20521 gagaaatcat tacggcagga ctttagcgga aaggataact accggttttg acggcacatt
    20581 ctggtacgga ccaacatcat gagtcggcac ctgggaaatc ttatcggcaa cgtccatgcc
    20641 tttcaccact ttaccaaata ccgcgtaacc gaaatcacgc tgaccatggt caaggaaggc
    20701 gttatcggca acgttgataa agaactggct ggtggcgctg tctttgtcag cggtacgcgc
    20761 catcgcgatg gtgccacgcg tgttgcgcag gccgttatcg gcttcatttt tgattggcgg
    20821 gtttggtttt ttctgctgca tctgctcggt gaaaccgccg ccctgaatca taaagccagg
    20881 aatgacgcgg tgaaaggtag tgttgttata aaaaccgctg ttcacataat cgacaaagtt
    20941 ttgcacagac actggcgctt tttgtttatc cagctccagt tcgatgttac cagctgaggt
    21001 tgtcaacaat acgtgcgggt cccctttcgc tgccattgct gcgggagaaa gagcagaaag
    21061 agcgaaaaca gcagccatcg ccgccagggt cgatttgaac atgagatttc cttaacaaac
    21121 agagcagaaa aaaagcaagt gaagagattc taaatagcct tcaagaacaa ggccatccct
    21181 ttacctaatt ttacgtatct gaaatatatg taacctgacg atcacttttt attccgtgat
    21241 caaaatcacc tcttaaaatg caatttagca accgattgca ataaaacatt taaacagatc
    21301 acaaaatcac ctaaaatcgc ccgctcgcgg catatcccgc tatgccattt tatttttgaa
    21361 cgcaggccaa tcatgactaa cagcaatcgc atcaagctca catggattag ctttctctcc
    21421 tacgcactga ctggtgcgtt ggttattgtc accgggatgg tgatgggaaa tatcgccgat
    21481 tatttcaatc tgcctgtttc cagtatgagt aacaccttca ccttcctcaa cgccggcatt
    21541 ttaatctcta tcttcctcaa cgcctggctg atggaaatcg tcccgttgaa aacgcagtta
    21601 cgttttggct ttctcctgat ggtgctggcg gttgccggtt tgatgttcag ccacagcctg
    21661 gcgctgttct cgacggcgat gttcattctc ggggtggtca gcggcattac tatgtcgatt
    21721 ggtacattcc tgataacaca aatgtatgaa gggcgtcagc gcggttcccg cctgttattt
    21781 actgactcct tcttcagtat ggctgggatg attttcccaa tgatcgccgc gtttctactg
    21841 gcgcgcagca ttgagtggta ctgggtttat gcctgcatcg ggctggtgta tgtcgctatt
    21901 tttattctga ccttcggctg tgagttcccg gcactgggca aacatgcacc aaaaacggat
    21961 gctccggtag agaaagaaaa gtgggggatc ggcgtactgt ttctctctgt tgcggcgctg
    22021 tgctacatcc tcggtcagtt aggttttatc tcctgggtgc ctgagtatgc caaaggcctg
    22081 ggcatgagcc tgaacgacgc gggcacgctg gtgagtaact tctggatgtc atacatggtc
    22141 ggcatgtggg cgttcagctt tattcttcgc ttctttgatt tgcaacgcat tctgaccgta
    22201 ctggctggtc tggctgcgat tctgatgtac gtctttaaca ccggaacacc agcacatatg
    22261 gcgtggtcaa ttctcgctct gggcttcttc tccagcgcga tctataccac catcatcacc
    22321 ctgggttcac agcagaccaa agtaccgtcg ccaaaactgg ttaactttgt cctgacctgc
    22381 ggaaccatcg gtactatgtt gacctttgtg gttaccggcc cgattgttga acatagcggt
    22441 ccgcaggcgg cactgcttac agcaaacggt ctgtacgctg tcgtctttgt gatgtgcttc
    22501 ctgttaggtt tcgtcagccg tcaccgtcag cataacaccc tgacctctca ttaattgcta
    22561 atgccggacg gcactatcgt cgtccggcct tttcctctct tcccccgcta cgtgcatcta
    22621 tttctataaa cccgttcatt ttgtccattt tttgcacaaa catgaaatgt cagacaattc
    22681 cgtgacttaa gaaaatttat acaaatcagc aacataccac ttaaggagta tataaaggtg
    22741 aatttgattt acatcaataa gcggggttgc tgaatcgtta aggtaggcgg taatagaaaa
    22801 gaaatcgagg caaaaatgag caaagtcaga ctcgcaatta tcggtaacgg tatggtcggc
    22861 catcgcttta tcgaagatct tcttgataaa tctgatgcgg ccaactttga tattaccgtt
    22921 ttctgtgaag aaccgcgcat cgcttatgac cgcgtacacc tctcgtctta cttctctcac
    22981 cacaccgccg aagagctgtc gctggtgcgc gaaggcttct acgagaaaca cggcatcaaa
    23041 gttctggtcg gcgaacgcgc tatcaccatc aaccgtcagg agaaggtgat tcactccagc
    23101 gccggacgta ccgtttttta tgacaagctg atcatggcaa ccggttccta cccgtggatc
    23161 ccaccaatca aaggttctga tactcaggac tgctttgtct atcgcaccat tgaagacctc
    23221 aacgccattg aatcctgcgc ccgtcgcagc aaacgcggtg ccgttgttgg tggcggcctg
    23281 ttaggtctgg aagccgctgg cgcgctgaaa aacttaggta ttgaaaccca cgttatcgaa
    23341 tttgccccta tgctgatggc ggaacagctt gatcagatgg gtggcgagca gctgcgtcgc
    23401 aaaatcgaaa gtatgggcgt gcgcgttcac accagcaaaa acacccttga gattgtgcag
    23461 gaaggtgtcg aagcgcgtaa aaccatgcgt tttgccgacg gcagcgaact ggaagtcgac
    23521 tttatcgtct tctctaccgg tatccgtccg cgcgataagc tggcaaccca gtgtggtctg
    23581 gacgttgctc cgcgtggggg tatcgtcatt aatgattcct gccagacttc cgatccggat
    23641 atctacgcca tcggtgaatg cgcaagctgg aacaaccgtg tatttggtct ggtagcacct
    23701 ggctacaaaa tggcgcaggt cgccgttgac catattctcg gtagcgaaaa cgcctttgaa
    23761 ggtgctgacc ttagcgccaa gctgaaactg ctgggcgtag acgtaggcgg tattggtgat
    23821 gcgcacggtc gcacgcctgg cgcacgtagc tacgtttacc tcgacgaaag taaagagatc
    23881 tacaaacgcc tgattgtcag cgaagacaac aaaaccctgc tcggtgcggt actggtgggc
    23941 gataccagcg actacggtaa cctgctgcaa ctggtgctga acgctatcga actgccggaa
    24001 aacccggatt ccctgatcct gccagcacac tctggtagcg gcaagccgtc tatcggtgtt
    24061 gataaactgc cggacagcgc gcaaatctgc tcctgcttcg acgtcactaa aggtgatctg
    24121 attgctgcca tcaacaaagg ctgccacaca gttgcggcgc tgaaagctga aaccaaagcg
    24181 ggtactggct gcggtggctg tatcccgctg gtcactcagg tactgaacgc ggaactggcg
    24241 aaacagggca tcgaagttaa caacaacctg tgcgaacact ttgcttattc gcgtcaggaa
    24301 ctgttccatt tgatccgcgt tgaaggcatt aaaaccttcg aagaactgct ggcgaaacac
    24361 ggcaaaggct acggttgtga agtttgtaaa ccaaccgtcg gttcgctgct ggcctcctgc
    24421 tggaacgaat acattctgaa gccggaacat actccgctgc aggattctaa cgacaacttc
    24481 ctcgctaaca tccagaaaga cggcacctac tcggtgatcc cgcgttctcc gggcggtgaa
    24541 atcaccccgg aagggctgat ggcagtgggt cgtatcgcgc gtgaatttaa tctctacacc
    24601 aaaatcaccg gctcccagcg cctggcgatg tttggcgcac agaaagacga tctgccggaa
    24661 atctggcgtc agctgattga agctggcttc gaaaccggtc atgcctatgc gaaagcactg
    24721 cgtatggcga aaacctgcgt gggtagcacc tggtgccgct acggtgttgg cgatagcgtc
    24781 ggcctcggcg tggaactgga aaaccgctac aaaggcatcc gtacgccgca caaaatgaag
    24841 ttcggtgtct ccggctgtac ccgtgaatgt tcagaagctc agggtaaaga cgtgggtatt
    24901 atcgccactg aaaaaggctg gaacctgtat gtttgcggta acggcggcat gaaaccgcgt
    24961 catgcggatc tgctggcagc ggatatcgat cgcgaaacgc tgatcaaata tctcgaccgc
    25021 ttcatgatgt tctacatccg taccgccgac aaactgacgc gtaccgcgcc gtggttagaa
    25081 aacctcgaag gcggcatcga ttacctgaaa gcggtgatca ttgacgacaa actggggctg
    25141 aacgctcatc tggaagaaga gatggcgcgc ctgcgtgaag cggtagtgtg tgagtggact
    25201 gaaacggtca atacgccgtc tgcgcagact cgcttcaaac acttcatcaa cagcgacaag
    25261 cgtgacccga acgtgcagat ggtgccagag cgcgaacagc accgtccggc aacgccgtat
    25321 gaacgtatcc cggtaactct ggtggaggac aacgcatgag ccagtggaaa gacatctgca
    25381 aaatcgatga catcctgcct gaaaccggcg tctgcgcgct gttaggtgac gagcaggtag
    25441 cgattttccg cccgtatcac agcgaccagg tgtttgcgat cagcaacatt gatccgttct
    25501 tcgagtccag cgtgctatca cgcgggctga ttgcggaaca ccagggcgag ctgtgggtcg
    25561 ccagcccgct gaaaaaacag cgttttcgct taagtgatgg cttgtgcatg gaagacgaac
    25621 agttttccgt caaacattac gaagcgcgag tgaaagacgg cgtggtgcag ctgcgcggtt
    25681 aatgttttaa cgggaggcgc aatgcctccc ctttttgcat ggtcctgtaa tgaccttcgg
    25741 tatattgcag aacatttttt aaactttttg ttttattttt tgtttttatt ttttaaagga
    25801 taatcaaatg tttacagaca ctattaataa gtgtgcggct aatgctgcgc gcattgcacg
    25861 cctgtcggca aataacccgc tcggcttttg ggtcagctcc gccatggcgg gcgcatatgt
    25921 gggtcttggg atcatcctga ttttcacgct cggtaatttg ctcgacccgt ccgtacgtcc
    25981 gctggtgatg ggcgcgacct ttggtatcgc cttaacgctg gtgattatcg ccggttctga
    26041 actgttcacc ggacacgcca tgttcctcac ctttggggta aaagcgggca gcatcagcca
    26101 cgggcaaatg tgggcaatcc tgccgcaaac ctggctgggt aacctggtcg gttccgtctt
    26161 cgttgccatg ctctatagct ggggcggcgg tagcctgctg ccggtagata ccagcatcgt
    26221 tcactccgtc gcgctggcta aaaccactgc accggcaatg gtactgttct tcaaaggtgc
    26281 attgtgtaac tggctggttt gcctggctat ctggatggcg ctgcgcactg aaggggcggc
    26341 gaaatttatc gctatctggt ggtgtctgct ggcatttatc gcgtccggct acgagcactc
    26401 catcgctaac atgacgctgt tcgccctctc ctggttcggc aaccacagcg aagcctacac
    26461 gctggcgggt attggtcata acctgctgtg ggtgacgctg ggtaatactt tatcgggtgc
    26521 cgtattcatg ggattgggtt attggtatgc tacgccgaaa gcgaatcgtc cggttgcgga
    26581 caaatttaat caaactgaaa cggctgccgg ttaattacta aggggttttt acgtggatca
    26641 tttgcctata ttttgccaat tacgcgatcg cgactgtctg attgtcggcg gtggtgatgt
    26701 cgcggaacgc aaagcaaggt tgctgttaga cgcaggcgct cgcttaacgg tgaatgcatt
    26761 agcgtttatt ccacagttca ccgcatgggc agatgcaggc atgttaaccc tcgtcgaagg
    26821 gccatttgat gaaagccttc tcgacacctg ctggctggcg attgcagcga cggatgatga
    26881 cacgcttaac cagcgcgtca gcgaagccgc tgaagctcgt cgcatcttct gtaacgtagt
    26941 cgatgcgccg aaagccgcca gctttattat gccgtcgatt attgaccgct caccgctaat
    27001 ggtagcggtc tcctctggcg gcacctctcc ggttctggcg cgcctgttgc gcgaaaaact
    27061 cgaatcactg ctgccgttgc atctgggcca ggtggcgaaa tatgccgggc aattacgcgg
    27121 gcgagtgaaa caacagttcg ccacgatgag tgagcgtcgc cgtttctggg agaaattgtt
    27181 cgttaacgac cgcctggcgc agtcgctggc aaacaacgat cagaaagcca ttactgaaac
    27241 gaccgaacag ttaatcaacg aaccgctcga ccatcgcggt gaagtggtgc tggttggtgc
    27301 aggtccgggc gatgccgggc tgctgacgct gaaaggactg caacaaattc agcaggcaga
    27361 tgtggtggtc tacgaccgtc tggtttctga cgatattatg aatctggtac gccgcgatgc
    27421 tgatcgcgtt ttcgtcggca aacgcgcggg ataccactgt gtaccgcagg aagagattaa
    27481 ccagatcctg ctgcgggaag cgcaaaaagg caaacgcgtg gtgcggctga aaggtggcga
    27541 tccgtttatt tttggccgtg gtggcgaaga gctggaaaca ctgtgcaacg cgggtattcc
    27601 gttctcggtg gttccgggta ttaccgcagc ttctggttgc tctgcctatt cgggtattcc
    27661 actcacgcat cgcgattatg cccagagcgt acgcttaatt accggacact taaaaaccgg
    27721 tggcgagctg gactgggaaa acctggcggc agaaaaacag acgctggtgt tctatatggg
    27781 attgaatcag gccgcgacta ttcagcaaaa gctgattgaa cacggtatgc ctggcgaaat
    27841 gccggtggca attgtcgaaa acggaacggc agtcacgcag cgcgtgattg acggtacgct
    27901 cacgcaactc ggtgaacttg ctcagcaaat gaacagtcca tcgctaatta ttattggtcg
    27961 ggttgttggc ctgcgcgata aactgaactg gttctccaac cattaattta acccgggcca
    28021 gagaattctg gccttcttaa tgactctttt tatatattca gcaaataaaa cataacccct
    28081 taataaatat tatctgctaa tgggttatat tcaccgttac agcgacttac aaaattaagt
    28141 cattcgctca ctgacgccaa ttaaatattc ctgtatcgtt cttaacgagc agtctgctta
    28201 tattcatata atcaatgaat attaattaat atataataca tagggaatgt aaatgaacaa
    28261 atttattaaa gttgcactgg taggtgcagt actggctacg ttaactgcat gtactggtca
    28321 tattgaaaac cgtgataaga actgctctta cgactacctg ctgcacccgg caatttctat
    28381 ttctaaaatc attggcggtt gcggtcctac tgcacagtaa gtctctgaca gaaatcggct
    28441 aacaccggtt taattgcccg acgccctctg gattttccga gggcgtattt gtttctatgt
    28501 ccttcttgcc cccgcccgta aaaatatttt cactttaaat tcaatttgat aactacatcc
    28561 attagccaca gcgcgatcct gtgcgaaatt ttgtgatctt cctccacatt acataacatc
    28621 atatgttgtt atattcatca tgcattgtca tgttaccttt taaatgactg caaactctcc
    28681 cctacaacgt attggacaag aaaaaggtat cgctat
//
LOCUS       DAJVRF010000039        28679 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJVRF010000039 DAJVRF010000000
VERSION     DAJVRF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28679
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.039"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..1046)
                     /locus_tag="OQN13_001576"
     CDS             complement(<1..1046)
                     /locus_tag="OQN13_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCS1614963.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..2094
                     /gene="pinE"
                     /locus_tag="OQN13_001577"
                     /pseudo
     CDS             1537..2094
                     /gene="pinE"
                     /locus_tag="OQN13_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2433..3281)
                     /locus_tag="OQN13_001578"
     CDS             complement(2433..3281)
                     /locus_tag="OQN13_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCS1614964.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3490..4125
                     /gene="yfhb"
                     /locus_tag="OQN13_001579"
     CDS             3490..4125
                     /gene="yfhb"
                     /locus_tag="OQN13_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCS1614965.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4150..4686
                     /gene="tadA"
                     /locus_tag="OQN13_001580"
     CDS             4150..4686
                     /gene="tadA"
                     /locus_tag="OQN13_001580"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCS1614966.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQPSTD"
     gene            complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="OQN13_001581"
     CDS             complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="OQN13_001581"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCS1614967.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6497..10384
                     /gene="purL"
                     /locus_tag="OQN13_001582"
                     /gene_synonym="purI"
     CDS             6497..10384
                     /gene="purL"
                     /locus_tag="OQN13_001582"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCS1614968.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            10942..12369
                     /gene="qseE"
                     /locus_tag="OQN13_001583"
     CDS             10942..12369
                     /gene="qseE"
                     /locus_tag="OQN13_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCS1614969.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12534..13247
                     /gene="qseG"
                     /locus_tag="OQN13_001584"
     CDS             12534..13247
                     /gene="qseG"
                     /locus_tag="OQN13_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCS1614970.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13237..14571
                     /gene="glrR"
                     /locus_tag="OQN13_001585"
     CDS             13237..14571
                     /gene="glrR"
                     /locus_tag="OQN13_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCS1614971.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14632..14970
                     /gene="glnB"
                     /locus_tag="OQN13_001586"
     CDS             14632..14970
                     /gene="glnB"
                     /locus_tag="OQN13_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCS1614972.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="OQN13_001587"
     CDS             complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="OQN13_001587"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCS1614973.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16533..17786
                     /gene="glyA"
                     /locus_tag="OQN13_001588"
     CDS             16533..17786
                     /gene="glyA"
                     /locus_tag="OQN13_001588"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCS1614974.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(17984..19177)
                     /locus_tag="OQN13_001589"
                     /pseudo
     CDS             complement(17984..19177)
                     /locus_tag="OQN13_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19295..22572
                     /locus_tag="OQN13_001590"
                     /pseudo
     CDS             19295..22572
                     /locus_tag="OQN13_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            22669..23652
                     /locus_tag="OQN13_001591"
     CDS             22669..23652
                     /locus_tag="OQN13_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCS1614975.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            23675..25186
                     /locus_tag="OQN13_001592"
     CDS             23675..25186
                     /locus_tag="OQN13_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS1614976.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            25211..26209
                     /locus_tag="OQN13_001593"
     CDS             25211..26209
                     /locus_tag="OQN13_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1614977.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            26242..27336
                     /locus_tag="OQN13_001594"
     CDS             26242..27336
                     /locus_tag="OQN13_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1614978.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            27348..28220
                     /locus_tag="OQN13_001595"
     CDS             27348..28220
                     /locus_tag="OQN13_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCS1614979.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(28268..>28679)
                     /locus_tag="OQN13_001596"
     CDS             complement(28268..>28679)
                     /locus_tag="OQN13_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS1614980.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6817 a         7093 c         7791 g         6978 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg caagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtga ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctactttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaatac cgatctacaa cgtaatgcgt tgaactgtgc aggatgcgag
     1621 ctgatttttg aagacaagat aagtggcaca aagtccgaaa ggccggggct gaaaaaactg
     1681 ctcaggacat tatcggcagg cgacacactg gttgtctgga agctggatcg gctggggcgc
     1741 agtatgcggc atcttgtcgt gctggtggag gagttgcgcg aacgaggcat caactttcgt
     1801 agtctgactg attcaattga taccagcaca ccaatgggac gctttttctt ccatgtgatg
     1861 ggtgccctgg ctgaaatgga acgtgaactg attgttgaac gaacaaaagc aggactggaa
     1921 gctgctcgcg catagggacg aattggcggt cgccgtccca aactttcacc agaataatgg
     1981 gcgcaggccg ggcgactaat tgcatcagga gtttctcgcc agaaggtggc gatcatctat
     2041 gatgttggcg aatcgacttt tttgtataag aggtttccag ccagggttat ataaaaccgc
     2101 agacacggcg tatacaagaa cgtgctacgg ttgactgaaa agttaatgtg gtatgtcaca
     2161 ggccttttgc gccgtaaagc cccgtccttt agggcgcgga ggacgttaaa gaaagtggtg
     2221 ttctttgtgt tgctgaactt atgcaaaatt tttttgcacc ataaatccaa caacacattc
     2281 aataacacat tgctgtatca ctatgaaaat aaatgtgttt ttatttgatt tcacacatat
     2341 cacaattgat gcatttttta gtgattaaca acgccataag gaaactctca aaacaacaca
     2401 aaccgggcgg gcattgtacg cgcattcttt actcagacca gttttttcac cagcgcttca
     2461 ctatgacgaa tacgttctgg tgccagttcc agatcctgct gaatcagcgc aatgaacagc
     2521 aaatccgtta acattccctg agcgtgacaa gcagagattg aagcactgtt tgtcgcttgt
     2581 tcttcggcaa tggtatacag gcaatgagaa gcacgctgtt gcagggcatt cggagtaaag
     2641 ccggtaatcg ccagcacttt tccgcccact cgcagcatct catctgccgc caggtttaac
     2701 tcgcgtcgta caccggtgta ggaaatggct aataacagat cgtcagggga cgacgcctgt
     2761 actgttgcga gtagcgcatg catatcgcgc actgcggcag cattgaagcc aatcttcatc
     2821 agcttccagg caaagttttg cgccaccaga cccgaagcgc caataccggt cagaattatc
     2881 cgccgcgcag agcgcaacat tgttacgcat tcatgcagtt tctcttcact attaacgttt
     2941 agcgttgcgt acatagcggc agtattttct ttaatcagtt tttcgccgac cagccgtaac
     3001 ggatcatcac cgcggatttg gttatgaatg ggcacggagg gcgattccgg ctggcttgcc
     3061 agcgcttcac tcaacgccaa cttaagcgcc ggaaaacctt tatagccgag tttttgcgcg
     3121 aacttcacga cgctggactg actgactccg gcttcgttgg ccagttgctg agagcttaaa
     3181 tggcgcgccg tatcaggttg tagcagcaga taatccgcca gttttttatc actctgggca
     3241 agcccctggt aacgctgacg gatacgaagt aagccgttca tatctttcct cgcgatgaaa
     3301 tagtggcgac aacaggcaat atgagacaca cgcgagtgaa ttttatattc cattgccgct
     3361 aattgccatg aattaaaaat tccagttagc catgatgcgg tcgattgttg ccggaaaagt
     3421 gatgatctat ctgttaggct atgtagtagc gaaaattaat caggatattt cagtccagag
     3481 gagtatggtt tggcaactca cgagcgtcgt gtggtgtttt ttgacttaga tggaacattg
     3541 catcagcagg atatgttcgg cagttttctg cgctatttac tgcgtcgcca accgctgaat
     3601 gcgttacttg tcctgccgtt gttaccgatt atagccattg cgttattgat aaaaggtcgt
     3661 gcggcacgct ggccgatgag tctgcttctg tgggggtgca cttttggtca cagcgaagca
     3721 cgtttacaga cgttgcaggc cgatttcgtg cgctggtttc gcgacaatgt taccgccttt
     3781 ccgctggttc aggagcgatt aaccacctac ctgttaagtt ccgatgctga tatctggttg
     3841 attaccggct ctccgcagcc gctggttgaa gcggtttatt tcgatacgcc ctggctgccg
     3901 cgggttaatc ttatcgccag ccaaattcag cgtggctatg gtggttgggt attgacgatg
     3961 cgttgtctgg gacatgaaaa ggtcgcacaa ctggagcgca aaatcggcac tccgctgcgg
     4021 ctgtacagtg gctatagcga cagtaatcag gacaatccgc tgctttattt ctgtcagcat
     4081 cgttggcgag taaccccgcg cggtgaactc cagcaactgg aatagagtaa agcatagcgt
     4141 ccgtgtataa tgcgccgcgc ttttataacc ggagttttct ttttgtctga agtcgaattt
     4201 agccacgaat actggatgcg tcacgcgctg acgctggcga aacgtgcctg ggatgagcgg
     4261 gaagtgccgg tcggcgcggt attagtgcat aacaatcggg taatcggcga aggctggaac
     4321 cgcccgattg gtcgccatga tcccaccgca catgcagaaa tcatggccct gcggcagggt
     4381 ggtctggtga tgcaaaatta tcgtctgatc gacgccacgt tgtatgtcac gcttgaacca
     4441 tgtgtaatgt gtgccggagc gatgatccac agtcgcattg gtcgcgtggt ctttggtgcg
     4501 cgtgacgcga aaactggcgc tgcgggatct ttaatggatg tgctgcatca tccgggtatg
     4561 aatcaccgag tggaaattac ggaaggaata ctggcggatg agtgcgcggc gttgctcagt
     4621 gacttctttc gcatgcgccg ccaggaaatt aaagcgcaga aaaaagcgca accctcgacg
     4681 gattaatttt gtttctcttc actccctttc ctggaaaaca gcagagaggg agaatgggtc
     4741 aaatagtttg atgacgactg ggaaagaaac gagagaaaag gaaatttctc tttgcccaac
     4801 tccgtaggcg ataccgccgg ataatcctgc gccagttgca tcgccgcttc tgtagcctgc
     4861 ttctctttct cttgcagata acccaccagg ctaatctgat acttacgaat attttcgaca
     4921 taagcgtagg cttcatgtcc acgagcgtag ccgtaagtca gcttgctgta atagggtttc
     4981 tggctaagta aaggcagacg ctgttttacg tcagcccaac tgtcaggatt ccctttggtt
     5041 tttgccgtca gggcgcgggc atccagcata tgcgcatagc ccatattgta cgcagcgagg
     5101 gcaaaccaga tccgctcgtt ctccggcaca ctttccggca ctttactcat catatcctgc
     5161 aaataacgca cgccaccgct gatgctctgt tcggcatcgg tacgatccgt aatgccgagg
     5221 ctttgcgcgg tatttttggt taacatcatc atgccgcgca cacccgtcgg tgaagtggcc
     5281 tgtgcatccc agtgcgattc ctgataagca atagcggcca gcaaacgcca gtcaatttct
     5341 tcggcgtatt tctcaaacag gggctttaac tgcggcagta ccgcatcgac ggcgcgtaaa
     5401 aatgtgcgcg tatcgacgta atcaaaatca tcgccatgcc ccaggtattt ctcttcaatg
     5461 cgtgccagcg taccgtcttc attcatttcg ttgaagaagt cgagcagggc ggcggaaagg
     5521 gtattatcgc catctaacgg gctaaaccaa gtcaccggtt gttcatcggt gatatcgagc
     5581 gctacggcga gctccgggtg aacgcgctga aacaggctga tggcgacaga atcagcaatg
     5641 gtgtaatcga gttttccttc gatgacatct tccattaatt ccgcagagcc ttttttgtcg
     5701 tctaccttcc agcttaattc cgggaatttt gtgtctttca gggtttggag atcgttaacc
     5761 accacgtgac ccggtgcaac ggtgagttgc tccgccgtca ggttgcccag tgtgcgcggg
     5821 cgatactgac tcactttata aaccagttgt tgtgacacgg aataataggt agggccaggc
     5881 tgataatttt ttacccgctc actgttatag accagtcctg ccgccagcag gtcggcgtta
     5941 ccattatcga ggtcgtcaaa cagctggctg atattctgcc gcacggtcac tttcagtttt
     6001 acaccgaggt aatcggcaaa ctgtttcgcc agttcgtaat ccaggccaaa aggtttcccg
     6061 ttgatttcgt tataagtcag gggagtatga atggtgctca cacgcaactc tccccgcgct
     6121 tgaatggcgg cgatacggtt gtcggcttta ccaaaccagg gaatggatgg ccagagagcg
     6181 accgcgagca gcagtgccag aatgccgatg aacagataat taatctttaa ttttttcaat
     6241 tagttaattc tctgtgtcgt gcgcgtccca gcttgaaaaa acgtaataat agtgaaaggt
     6301 ttactcataa atgagcggca ttttgcgtaa acctgcgcca gatggcaact tattacagcc
     6361 attggcggca cgcgttgcta attcacgatg gtgattttat ttccacgcaa acggtttcgt
     6421 cagcgcatca gattctttat aatgacgccc gtttcccccc cttgggtaca ccgaaagctt
     6481 agaagacgag agacttatga tggaaattct gcgtggttcg cctgcactgt cggcattccg
     6541 aatcaacaaa ctgctggcac gttttcaggc tgccaggctc ccggttcaca atatttacgc
     6601 cgagtatgtc cattttgctg acctcaatgc gccgttaaac gatgatgagc acgcacaact
     6661 tgaacgcctg ctgaaatatg gcccggcact cgccagccac gccccgcaag gcaaactcct
     6721 gctggtgacc ccgcgtcctg gcaccatctc tccctggtct tcgaaagcga ccgatattgc
     6781 ccataactgc gggctacaac aggtaaaccg ccttgagcgc ggcgttgctt actatataga
     6841 agccggtacg ctgaccaatg aacaatggca gcaggttacc gctgaactgc acgaccgcat
     6901 gatggaaacg gtcttttttg ctttagatga tgcagagcag ttgtttgccc accatcaacc
     6961 gactccggtt accagcgttg atttgctggg gcagggccgt caggcgctga tcgacgctaa
     7021 cctgcgtctt ggcctggctc tggcggaaga tgaaattgac tatctgcagg atgctttcac
     7081 aaagcttggt cgtaacccga acgacatcga gctgtatatg ttcgctcagg cgaactctga
     7141 gcactgccgc cacaaaattt ttaatgccga ctggattatc gacggtgaac agcagccgaa
     7201 atcgctgttc aagatgatca aaaatacttt cgaaaccacg ccagatcacg ttctctctgc
     7261 ttataaagat aacgccgccg taatggaagg ttctgaagtg ggccgctact ttgctgacca
     7321 cgaaacgggc cgctacgatt tccatcagga accggcgcat attctgatga aagtcgaaac
     7381 tcacaaccac ccgacggcga tttctccgtg gccgggcgcg gcgacgggtt ccggcggcga
     7441 aattcgtgat gaaggcgcca ccggacgcgg tgcgaagccg aaggctggtc tggttggttt
     7501 ctccgtttcc aacctgcgaa ttcctggctt cgaacagccg tgggaagaag atttcggtaa
     7561 gcctgagcgc attgtcaccg cgctggacat catgaccgaa ggcccgctgg gcggcgcggc
     7621 gtttaacaac gaatttggtc gtccggcact gaatggctac ttccgtactt atgaagaaaa
     7681 agtgaacagc cacaacggcg aagagctgcg cggttatcac aaaccgatca tgctggcggg
     7741 cgggatcggc aacattcgcg ccgatcacgt acaaaaaggc gagatcaacg tcggtgcgaa
     7801 gctggtcgtt ctcggcggcc cggcaatgaa catcggtctt ggcggcggag cggcgtcttc
     7861 catggcgtca ggtcagtctg atgccgacct cgactttgct tccgtacagc gcgacaaccc
     7921 ggagatggag cgtcgctgcc aggaagtgat cgaccgttgc tggcagcttg gtgatgccaa
     7981 cccaatcctg tttatccacg acgtcggcgc tggcggtctt tctaacgcga tgccggaact
     8041 ggtgagcgac ggcgggcgcg gcggtaaatt tgaactgcgc gatattctaa gcgacgaacc
     8101 gggcatgagc ccgctggaaa tctggtgtaa cgaatcccag gaacgctacg tgctggcggt
     8161 tgctgccgat caattaccgc tgtttgacga actgtgtaag cgtgagcgcg caccctacgc
     8221 ggtgattggt gaagcgaccg aagaactgca tctttctctg cacgatcgtc attttgataa
     8281 tcagccgatc gatctgccgc tggacgtcct gcttggtaaa acgccgaaga tgacccgcga
     8341 tgtacaaacg ctgaaagcga agggcgatgc gctggtccgt gaagggatca ccattgcaga
     8401 cgcggtgaaa cgcgtactgc atctgccgac cgtggcagag aaaaccttcc tggtgaccat
     8461 cggcgaccgc agcgtaaccg gcatggtagc gcgcgatcag atggtggggc cgtggcaggt
     8521 gccggtcgct aactgcgcgg tcactaccgc cagcctcgac agctactacg gtgaagcgat
     8581 ggcgattggc gagcgtgcgc cggttgcact gctggatttc gccgcctctg cccgtctggc
     8641 ggtcggtgaa gcgttaacca atattgccgc aacacagatt ggcgatatca aacgcattaa
     8701 actttccgcc aactggatgg cggcggcagg ccaccctggt gaagatgcgg gcctgtatga
     8761 agccgttaaa gccgtgggcg aagagctttg tccggcgctg ggcctgacga tcccggtggg
     8821 taaagactct atgtcgatga aaacccgctg gcaggaaggt aacgaagagc gcgaaatgac
     8881 gtcgccgctg tcgctggtga tttctgcatt tgcccgcgtg gaagatgtgc gtcacaccat
     8941 cacgccacag ctttctaccg aagataacac actgctgctg attgatttgg gcaaaggcaa
     9001 taacgcgctg ggcgcaacgg cgctggcgca ggtttatcgt cagcttggcg acaaaccggc
     9061 agatgtacgc gacgtcgcgc aactgaaagg cttctatgac gcgattcagg cgctggttgc
     9121 acagcgtaag ctgctcgcat accacgaccg ctctgatggc ggcctgctgg taacgctggc
     9181 ggaaatggcc tttgctggtc attgcggcat taacgcggat attgcctctc tgggcgacga
     9241 tcgcctggcg gcgttgttta acgaagaact gggtgcggtg attcaggttc gtgccgctga
     9301 ccgtgaagcg gtcgagtccg tactggcaca gcatgggctt gctgattgtg tccattatgt
     9361 agggcaggcg gtttccggtg accgttttgt gattaccgcc aacgggcaga ctgtattcag
     9421 cgaaagccgc accacgttgc gtgtctggtg ggcagaaact acctggcaga tgcagcgcct
     9481 gcgtgacaac ccggagtgtg ctgaccagga gcaccaggcg aaatcaaacg acgccgatcc
     9541 gggcctgaat gtgaaactgt cgttcgatat caacgaagat gtggcagcac cgtatattgc
     9601 cactggcgca cgtccgaaag ttgctgtgct gcgtgagcag ggcgtgaact cgcatgttga
     9661 aatggcggca gctttccacc gtgcaggctt tgatgctatc gacgtgcata tgagtgacct
     9721 gctgaccgga cgcacgggcc tggaagattt ccacgccctg gtcgcgtgcg gtggtttctc
     9781 ctacggtgat gtgctgggtg ccggtgaagg ttgggcgaag tcaatcctgt tcaatgaccg
     9841 tgtacgcgat gagtttgcaa ccttcttcca ccgtccgcaa acgctggcgc tgggggtatg
     9901 taacggttgc cagatgatgt ctaacctgcg tgaactgatc ccggggagcg agttgtggcc
     9961 acgttttgtg cgtaacacct ccgatcgctt tgaagcgcgt ttcagcctgg ttgaagtaac
    10021 ccaaagcccg tctctgctgt tgcaggggat ggtgggctcg caaatgccga ttgctgtctc
    10081 tcatggtgaa gggcgcgtgg aagtgcgtga tgcggcgcat ctggcggcac tggaaagcaa
    10141 agggctggtg gcactgcgct atgtcgataa cttcggcaaa gtcactgaaa cctacccggc
    10201 taacccgaac ggttcaccga acggtattac ggcggttacg actgaaagtg gtcgagtcac
    10261 cattatgatg ccgcacccgg aacgtgtttt ccgtactgtc agcaactcct ggcatccgga
    10321 aaactggggc gaggatggcc catggatgcg cattttccgc aatgcgcgta agcagttggg
    10381 gtaagtcatc agttcattgg ttttgcagcc cggatgcggc attaagcacc aaccactatc
    10441 ataagctccc aactccggga gcttttttgt gtctgtaaat cacgacaatg ggtggtttac
    10501 cgtgtcgctt tctggcgaca cttaaatgat tgattttatt gcaatctaat aagtttatct
    10561 taagttgtct ctttttagcg acacagtggc tgatttacat tcaaatagcg aagaagccat
    10621 aaatccctca aagcaacgta taatcagtac gttaccaaac tattttcttt attggcacag
    10681 ttactgcata atagtaagca gtggctcatt caccgactta tgtcagcccc ttcgggacgt
    10741 gctacataaa attcgaatga cgcacaacaa ggtgcctgcc gtccaacttc tgatatcagc
    10801 gtagctatat caaccatcgg gcgaaacgtc gagttaggca ccgccttatt ccataacaaa
    10861 gccgggtaat tcccggcttt gttgtatctg aacttcccct cggttagcat caggctattc
    10921 gcgtctgacg agagtaacac cttgaaacgc tggcccgttt ttccccgctc attacgacaa
    10981 ctggtaatgc tggcattttt gctaattcta ctacccctgt tggtgctggc atggcaagcc
    11041 tggcaaagcc tgaatgcgct tagcgatcag gcggcgctgg ttaaccgcac tacgcttatt
    11101 gatgcccggc gtagtgaagc gatgaccaac gcggcgctgg agatggagcg tagctaccgt
    11161 cagtattgcg tgctggacga ccctacgctt gcgaaggttt atcaaagcca gcgcaagcgt
    11221 tacagcgaaa tgctcgatgc ccacgcaggc gtactgccag acgataaact ttaccaggca
    11281 ttgcgtcagg atttgaacaa tctggcccaa cttcagtgta acaacagtgg tcccgatgct
    11341 gccgccgccg cgcgtcttga agcctttgcc agtgccaata ccgaaatggt gcaggccacg
    11401 cgcacagtgg tgttctctcg tgggcagcaa cttcagcgtg aaatcgccga acgtgggcaa
    11461 tattttggtt ggcaatcgct ggtgctattt ctggtgagtc tggtgatggt gctacttttc
    11521 acgcggatga ttatcgggcc ggtgaaaaat atcgagcgca tgatcaaccg tctgggggaa
    11581 gggcgttctc tgggcaatag cgtttcgttc agtggaccga gcgagttacg ctcggttggg
    11641 caacgtattc tttggttaag tgagcgcctg tcatggctgg aatcccaacg ccatcaattt
    11701 ttaagacatt tatctcatga attaaaaacg ccactggcga gtatgcgcga gggcactgaa
    11761 ttactggctg accaggttgt cgggccgctt acgccagagc aaaaagaggt ggtgagcatt
    11821 cttgatagca gcagccgcaa tttgcaaaaa ctgatcgaac aactgcttga ttacaaccgt
    11881 aaacaggcgg acagtgcggt ggaactggag aatgttgaat tagcaccgct ggtggagaca
    11941 gtggtttctg ctcatagcct gcccgcacgg gctaaaatga tgcataccga cgtcgatctc
    12001 aaagcaacag cttgcctggc ggagccaatg ctgctgatga gcgtactgga taatctttac
    12061 tccaatgcgg tgcactacgg ggctgaatcc ggtaacattt gccttcgcag cagttcacat
    12121 ggtgcgcggg tttatattga tgtcatcaat acaggcacgc ccattccgca agaggaacgc
    12181 gccatgatct tcgaaccctt ttttcaggga agccaccagc gaaaaggggc ggtgaagggc
    12241 agcggtctgg gattaagcat tgccagggat tgtattcgcc gtatgcaagg ggaactgtat
    12301 ctggtcgacg agagcgggca agacgtttgt ttccgcattg aattaccgtc gtcgaaaaac
    12361 acgaaataat gctaacagcc tatcctatca ggctatttta tttgtcagtt cggccccggg
    12421 cagtgctcga aatcttcacg tacgatatgt acgctccggt ttctccgcgc tgtccatgtc
    12481 cagactgtct gcaacaatta cgcccactgg gataggcttt aagtctggtg aatatgcgac
    12541 acatttttca acgattattg ccccgacggc tgtggctggc agggctgcca tgtctggcat
    12601 tgctgggttg cgtgcagaat cacaataagc cagccattga tacgccagca gaagaaaaaa
    12661 ttccggttta tcaactggct gactatcttt ctactgaatg tagcgacatc tgggcgctgc
    12721 aaggtaaatc aaccgaaacc aatccgcttt actggctgcg ggcgatggat tgtgctgatc
    12781 gtttaatgcc tgcgcagtcg cgtcagcaag ctcgccaata tgacgacggc agctggcaaa
    12841 atacctttaa acaagggatt ttgctcgccg acgccaaaat tacgccgtat gagcgccgcc
    12901 agctggtcgc gcgtattgaa gcgttaagta ccgagatccc ggcacaggtt cgcccactgt
    12961 atcaactctg gcgcgacggt caggcgctgc aactgcaact ggcggaagag cgccaacgtt
    13021 acagtaaact ccagcagtcc agcgacagcg agctggatac attgcgccag caacatcacg
    13081 ttctacagca gcaactggaa ctcaccaccc gcaagctgga aaacctgacc gatattgaac
    13141 gccagctctc gacccgcaaa ccgactggaa atttctcgcc ggatacgccg cacgagagtg
    13201 aaaagcccgc gccatccacc catgaggtca ctcctgatga gccataaacc tgcgcattta
    13261 ttattggtcg atgacgatcc gggattgctg aaactgcttg gcctgcgcct gaccagcgaa
    13321 ggctacagtg tggtcacggc ggaaagtggc gctgaaggat tacgggtact gaatcgcgaa
    13381 aaagtagatt tagttatcag cgacctgcgg atggatgaaa tggacggtat gcagctattt
    13441 gctgaaatcc agaaagtgca gccgggaatg ccagtaatta ttcttactgc gcatggttct
    13501 atccccgatg ccgttgctgc aacacagcag ggcgttttta gtttcctcac caagcctgtc
    13561 gataaagatg cgctatatca ggcaattgac gatgcgctgg agcaatccgc gccagccacc
    13621 gatgaacgct ggcgcgaggc aattgtcacc cgcagcccgc tgatgctgcg tttgctggaa
    13681 caggcgcggc tggtggcgca atcagacgtc agcgttttga ttaacggtca gagcggcacc
    13741 gggaaagaga ttttcgccca ggctatccac aacgccagcc cgcgcaacag caaaccattt
    13801 attgctatta actgtggcgc attgcccgag caattgctgg agtcggagct gtttggtcat
    13861 gcgcgtggcg cgtttactgg cgctgtcagc aatcgcgaag gtttattcca ggcggcggaa
    13921 ggcggaacgc tatttctcga tgaaattggc gatatgcctg cgccgttaca ggtcaaactg
    13981 ctgcgcgtgt tgcaggagcg taaagtgcgc ccgctgggca gtaaccgcga tattgatatc
    14041 aatgtgcgga ttatttctgc cactcaccgt gatctgccaa aagcgatggc gcgcggggaa
    14101 ttccgcgaag atctctatta ccgcctcaac gttgtcagcc tgaaaattcc ggcactggcg
    14161 gagcgcacag aagacattcc gctactggca aatcacctgt tgcgccaggc ggcagagcga
    14221 cataaaccgt ttgtccgcgc gttctctacc gatgcgatga aacgcctgat gaccgcgagc
    14281 tggccgggta atgtgcgcca gttggtcaac gtgattgaac agtgcgtggc gctgacctca
    14341 tctccggtga ttagtgatgc gctggtggag caggcgctgg agggtgaaaa tacggcgctg
    14401 ccaacctttg ttgaggcacg taatcagttt gaactcaact atttgcgtaa gctgctgcaa
    14461 atcaccaaag gcaacgtcac ccacgcggcg agaatggcgg ggcgcaaccg gacagaattt
    14521 tataaactgc tttcccgaca cgagctggat gcaaacgatt tcaaggaatg aattggcgtt
    14581 atgtgttacg tttagcagat caaaagacag gcgacctttt caaggaatag catgaaaaag
    14641 attgatgcga ttataaaacc cttcaagctg gacgatgtcc gcgaagcact ggccgaagtc
    14701 ggtattaccg gcatgacggt gaccgaagtg aaaggctttg gtcgccagaa aggccatacc
    14761 gagctgtacc gcggcgcgga gtatatggtg gattttctgc cgaaagtgaa aattgagatt
    14821 gtcgtaccgg acgacattgt cgatacctgt gtcgatacca ttattcgcac ggcgcaaacc
    14881 ggcaaaatcg gtgacggtaa aatcttcgtc tttgacgtgg cacgggtcat tcgcatccgt
    14941 accggtgaag aggacgacgc ggcaatttaa accgcgtcaa gggttgccgg atgtttccat
    15001 ccggcaacat caaattacag caccttatgc gggccaaagc attcgtaatg aatgttttcc
    15061 tgcttcacgc ccagatccac taactgtttc gcggtaaact gcatgaagcc aaccgggccg
    15121 catagataga actgcattgt cggatcgctg aacgcacctt ccagtttgct caaatccatc
    15181 agaccttcgc tatcaaactg acctttagcg cgatcggctt cgctcggctg acgatgccag
    15241 gtgtgcgcgg taaagcgcgg cagtgactgc cccagttcct taacttcatc ggcaaaggcg
    15301 tgaacatcgc cattttctgc cgcatggaac cagttcactt gtgctgtgtg gcctgctttt
    15361 gccagcgtgt cgagcattgc cagcattggc gtttgaccaa caccggcaga gattaacgtc
    15421 actggtgtgt catctgcgac agccataaag aaatcacctg ccggagcgac cagtttcacg
    15481 acatcgccaa cattggcgtg attgtgcaac cagttggata cctgcccacc ctcttcgcgt
    15541 ttcaccgcaa tacgatagcc tttgccatcc ggtttgcgag tcaaagagta ctgacgaatt
    15601 tcctgatgcg ggaaaccttc cggcttcagc cagacgccga gatattgccc cggacggtat
    15661 tctgccactg cgccaccgtc gaccggctcc agttcgaagc tggtgataag cgcgctgcgc
    15721 ggtgttttag ccacaatgcg gaaatcgcga gtaccttccc aaccaccggc tttgctggcg
    15781 ttttcgttat agatttccgc ctcgcgattg ataaatacat tagccagtac accataggct
    15841 ttaccccacg cgtccagcac ttcctgcccc gggctgaaca tttcgtccag cgttgccaac
    15901 aggtgttcac cgacgatgtt gtactgttcc ggtttgatct ggaagctggt gtgcttctgc
    15961 gcgatttttt ctaccgctgg cagcagcgca ggcaggtttt caatattact ggcgtaggcg
    16021 gcaatagcgt taaacagggc ttcacgttga tcgccattac gctggttact catgttaaaa
    16081 atttctttga gttctgggtt atgagtaaac atacggtcgt agaaatgggc ggttaacttt
    16141 ggccccgttt ccaccagtaa agggatggtg gcttttactg tagcgatggt ttgagcgtca
    16201 agcatatggt cttccttttt ttgcatctta attgatgtat ctcaaatgca tcttataaaa
    16261 aatagccctg caatgtaaat ggttctttgg tgtttttcag aaagaatgtg atgaagtgaa
    16321 aaatttgcat cacaaacctg aaaagaaatc cgtttccggt tgcaaactct ttattctcca
    16381 aagccttgcg tagcctgaag gtaatcgttt gcgtaaattc ctttgtcaag acctgttatc
    16441 gcacaatgat tcggttatac tgttcgccgt tgtccaacag gaccgcctat aaaggccaaa
    16501 aattttattg ttagctgagt caggagatgc ggatgttaaa gcgtgaaatg aacattgccg
    16561 attatgatgc cgaactgtgg caggctatgg agcaggaaaa agtacgtcag gaagagcaca
    16621 tcgaactgat cgcctccgaa aactacacca gcccgcgcgt aatgcaggcg cagggttctc
    16681 agctgaccaa caaatatgct gaaggttatc cgggcaaacg ctactacggc ggttgcgagt
    16741 atgttgatat cgttgaacaa ctggcgatcg atcgtgcgaa agaactgttc ggcgctgact
    16801 acgctaacgt ccagccgcac tccggctccc aggctaactt tgcggtctac accgcgctgc
    16861 tggaaccagg tgataccgtt ctgggtatga acctggcgca tggcggtcac ctgactcacg
    16921 gttctccggt taacttctcc ggtaaactgt acaacatcgt tccttacggt atcgatgcta
    16981 ccggtcatat cgactacgcc gatctggaaa aacaagccaa agaacacaag ccgaaaatga
    17041 ttatcggtgg tttctctgca tattccggcg tggttgactg ggcgaaaatg cgtgaaatcg
    17101 ctgacagcat cggtgcttac ctgttcgttg atatggcgca cgttgcgggc ctggttgctg
    17161 ctggcgtcta cccgaacccg gttcctcatg ctcacgttgt tactaccacc actcacaaaa
    17221 ccctggcggg tccgcgcggc ggcctgatcc tggcgaaagg tggtagcgaa gagctgtaca
    17281 aaaaactgaa ctctgccgtt ttccctggtg gtcagggcgg tccgttgatg cacgtaatcg
    17341 ccggtaaagc ggttgctctg aaagaagcga tggagcctga gttcaaaact taccagcagc
    17401 aggtcgcgaa aaacgctaaa gcgatggtag aagtgttcct cgagcgcggc tacaaagtgg
    17461 tttccggcgg cactgataac cacctgttcc tggttgatct ggttgataaa aacctgaccg
    17521 gtaaagaagc agacgccgct ctgggccgtg ctaacatcac cgtcaacaaa aacagcgtac
    17581 cgaacgatcc gaagagcccg tttgtgacct ccggtattcg cgtgggtact ccggcgatta
    17641 cgcgtcgcgg cttcaaagaa gcagaagcga aagaactggc tggctggatg tgtgacgtgc
    17701 tggacagcat caatgatgaa gccgttatcg agcgcatcaa aggtaaagtt ctcgacatct
    17761 gcgcacgttt cccggtttac gcataagcga aacggtgatt tgctgtcaat gtgctcgttg
    17821 ttcatgccgg atgcggcgtg aacgccttat ccggcctaca aaactttgca aattcaatat
    17881 attgcaatat ccgtgtaggc ctgataagcg tagcgcatca ggcaatctta ggtatgtgat
    17941 catcaaggct tccttcggga agcctttcta cgttattgcg ccatcaaatc tgtcgtaact
    18001 gcgcctcaac atacaaatag ccaatcccca acacctgttg tgcgcggctt aattgcccaa
    18061 agccaatttg cgttgctttc acgctcggcc cttcgtcgcg gtcgaacggg ttaaatcccg
    18121 tctggcgaat aatggtatta agccagtgct caccaaaggc acaactgcga ccgtacaacc
    18181 aaatctgatt aatattgagg atgttgagga agttatacag actcaaacca atggcattgg
    18241 cactgcgatc aacccaactg gtgatccacg gttctccact ctaccaggca gcgattaact
    18301 gcgctgtagt cagtttttcc ggatcaagct gagtattaac cggttgtgat tttagccata
    18361 cccgcgcctg tttttttaat gcacttaacg aggcgacggt ttccaggcag ccgtaacgtc
    18421 cacagtcgca gacgacgcca tccggattaa cgatggtgtg actaatctgt ccgctaccat
    18481 acaaactgcc gcgataaatt tgctcgttaa tcacgaacga cgagccaatg ccgtaatcaa
    18541 cgttgatcac gcagaaatcc cgtttctgcg aattattttg ccatttctcc gccagcgcca
    18601 gcatcacgca gtcattatcg accatcaccc gaatgccgag cttctcttcc agcagatact
    18661 tcacctcaac cggcgttgtc cacggcgctt gcggcatggt ttgcgacacg ccggtcaccg
    18721 gatcaacctg accgtggatt gccagcgcca gattgatggt gcggtccggc cacaatttac
    18781 ggtggcgatg ccagcatttt tcgatttcgg acagcagcgc ctgcggcgtc ggtgcatcaa
    18841 tctgtaaata ctcaaattca cctttcggac ttaaacaagc attagcaacc tgacactcaa
    18901 tactggtggg cgtcacgttc aggcacagcg tccagtcacc ttccggcgca atcagccatg
    18961 taccgctact atgcccgcgc gtctggcttt catcgtcgat attcaccacc cgtttttcgc
    19021 tttccagttc ctgcaaaata ttactgactg ccggaatcga aatttgcgcc agccgggcca
    19081 gcgttgattt attggcgcgc ttttgccggt acagcagttc cagaatcacg catttgttat
    19141 ggtggcgaat ctgttgattg ttaatgcagg ctctcatctt cactaaacct tgtttagtta
    19201 attgcgtgat tattccgcga aaacattgcc agattgttca atacactgcc acaaatcttt
    19261 taattcagta tgtcttgtta atattgaggg caccatgact ccagtaaaag tgtggcaaga
    19321 gcgcgttgag atcccgacct atgaaaccgg gccgcaggat atacatccca tgttcctgga
    19381 aaatcgcgtt tatcagggat cgtccggcgc ggtttatccc tacggcgtga ccgatacgct
    19441 gagcgagcag aaaaccctga aatcctggca ggcggtgtgg ctggaaaacg actacatcaa
    19501 agtgatgatc ctgccggaac tgggcggtcg ggtgcatcgc gcgtgggata aagtgaaaca
    19561 gcgcgatttt gtttatcaca atgaagtcat taaacctgcg ctggtggggc tgctggggcc
    19621 gtggatttcc ggtgggattg agtttaactg gccgcaacat catcgcccga ccacctttat
    19681 gcccgttgat ttcaccctcg aagcccatga agacggtgca cagacggtgt gggtaggcga
    19741 aacggagccg atgcatggtt tacaggtgat gacaggtttc accctgcgcc ctgggcggcg
    19801 ctggaaatcg ccagccgcgt ctataacggc aacgccacgc cgcgtcattt cttgtggtgg
    19861 gccaacccgg cagtgaaagg gggtgaaggg catcagagcg ttttcccacc ggatttgact
    19921 gcggtgtttg atcacggcaa acgggccgtc tccgctttcc ccatcgctac cggcacttac
    19981 tacaaagtgg actactccgc tggagtggac atttctcgct ataaaaatgt gcccgttcca
    20041 acctcatata tggccgaaaa atcgcagtac gattttgtcg gcgcgtggtg tcacgatgaa
    20101 gatggcggtt tgctgcacgt tgccaaccac catattgcgc caggtaaaaa acagtggagt
    20161 tggggacaca gtgaatttgg ccaggcgtgg gataagagtc tgaccgacaa taacggcccg
    20221 tatatcgaac tgatgaccgg tatttttgcc gataaccagc ccgattttac ctggcttgat
    20281 gcttacgaag agaagcgttt tgagcagtat ttcctgcctt atcattctct gggcatggtg
    20341 caaaatgcct cccgcgatgc ggtgataaaa ctccagcgta gtgagcaggg gattgagtgg
    20401 gggctgtatg ccatctctcc gttgaacgga taccgcctgg cgatccacga aatcggcaaa
    20461 tgcaacgcgt tacttgatga tgccgtggca ctgatgcctg cgaccgccat ccagggcgtg
    20521 ttgcacggta tcaatcctga taggctgacc attgagcttt ccgatgccga cggcaatatt
    20581 gtactgagtt atcaggaaca tcagccgcaa gcgttgccgt taccggacgt cgccaaagcg
    20641 ccactggcag cacaagacat taccagtaca gatgaagcct ggtttatcgg tcagcatctg
    20701 gagcaatatc atcacgccag ccgttcaccg ttcgattact acctgcgcgg cgtggcgctg
    20761 gacccgctgg attatcgctg taacctggcg ctggcgatgc tggaatataa ccgcgcagat
    20821 ttcccgcaag cggtggcgta tgccagtcag gcgctgaaac gcgcacatgc gctgaacaaa
    20881 aacccgcagt gtggacaagc aagtttgatt cgcgccagtg cttacgaacg tcagggacaa
    20941 tatcaacaag ccgaagagga tttctggcgt gcggtctgga gcggcaacag caaagccggt
    21001 ggctattatg gcctggcacg actggcggcg cgtaatggta acttcgacgc tggtctggat
    21061 ttttgccaac aaagtcttcg cgcctgccca accaatcagg aagtgctttg cctgcataat
    21121 ctgctgctgg tgttaagtgg tcgtcaggac aacgcgcgtt tgcagcgcgc gaaactgctg
    21181 cgtgattatc cgctgaacgc cattctgtgg tggctgaact ggttcgatgg tcgtagcgaa
    21241 tcagccctcg cgcagtggcg cggtctgtgt cagggacgcg acgttaacgc cctgatgacc
    21301 gccgggcaac tgattaactg gggaatgccc actctggcga cagagatgct gaacgcactg
    21361 gactgtcagc gcacgttatc gctttatcta caagccagct tgctgccgaa agccgaacgc
    21421 ggcgaactgg tcgcaaaagc cattgatgtc ttcccgcagt ttgtccgttt cccgaatacg
    21481 ctggaagaag tggcggcgct ggagagtatt gaagagtgct ggtttgcccg tcatttactg
    21541 gcctgtttct actacaacaa gcgcagctac aacaaagcca ttactttatg gcaacgttgc
    21601 gtagagatgt cgccggagtt tgccgatggc tggcgcgggt tggcgatcca tgcgtggaat
    21661 aagcaacacg attatgaact ggccgcgcgt tatcttgata atgcttatca gcttgcgccg
    21721 caggatgcac gtctgctttt cgaacgggat ttgctggata agttaagtgg agccacaccg
    21781 gagaaacgac tggcgcgtct ggaaaataat ctggaaattg cgctgaaacg cgacgacatg
    21841 accgcagaac tgctcaattt gtggcatctc acgggtcagg cagacaaagc ggcggacatt
    21901 ctcgccacgc gcaaattcca cccgtgggaa ggcggggaag ggaaggtcac cagtcagttt
    21961 atcctcaacc agttattacg cgcctggcag catcttgatg ccagacagcc gcagcaggcc
    22021 tgcgaactgc ttcatgccgc gctgcattat ccggagaatt taagcgaagg ccgtttaccg
    22081 gggcaaactg ataacgacat ctggttctgg caggcgatat gcgccaacgc gcagggcgat
    22141 gaaactgaag cgacgcgttg tttacgtctg gcggcgaccg gcgatcgcac cattaacatc
    22201 cacagttatt acaacgatca gccggttgat tatctcttct ggcaaggaat ggcgctgcga
    22261 ctgctgggcg aacaacaaat cgcacagcaa ctgtttagtg aaatgaaaca gtgggcgcaa
    22321 gagatggcga aaaccagtat tgaggcggat ttctttgctg tttcacaacc tgacctgttg
    22381 tcgctgtatg gcgatttaca acagcagcat aaagaaaaat gcctgatggt ggcgatgctg
    22441 gcgtccgcgg gactcgggga ggttgcgcaa tatgaatctg ctcgcgctga attgacggcg
    22501 attaatccgg cctggccgaa agcggcatta ttcaccaccg tgatgccttt tatttttaac
    22561 tacgttcact aaaccctatt aactgtaata tttgagcggc acgcgctgcc gctcatcaca
    22621 aaataattag aataagccgt tgttaataaa aactctacag gaggcactat gcctaaaaaa
    22681 atgagaacaa cccgtaatct attattaatg gctacgctat taggtagcgc actatttgcc
    22741 agggctgcgg ataaagaaat gaccattggt gcaatatacc ttgataccct gggatattac
    22801 gctggagtgc gtcagggcgt tcaggatgcg gcaaaagatt cttcagtaca ggtacagtta
    22861 attgaaacta acgcccaggg tgatatttcg aaagaaagta cctttgttga taccctcgtg
    22921 gcgcgtaatg tcgatgccat tattttatcg gcagtgtctg aaaatggcag tagccgtacc
    22981 gttcgtcgcg ccagtgaagc gggtattccg gtgatttgct acaacacctg tattaatcaa
    23041 aagggtgtcg ataaatatgt ctcggcgtat ctggtcggcg atccactgga atttggtaaa
    23101 aaactgggta acgctgccgc cgattatttt attgccaata aaattgacca gccgaaaatt
    23161 gccgtcatca attgcgaagc ctttgaagtt tgtgtgcagc gacgtaaagg atttgaagaa
    23221 gtattaaaat cccgcgttcc cggcgcgcaa attgtcgcta atcaggaagg gactgtttta
    23281 aataaagcga tttccgttgg tgaaaaactg attatctcca cgccggatct caacgccatt
    23341 atgggggagt caggcggtgc gacactcggc gcggtaaaag cggtacgtaa tcaaaatcag
    23401 gccggaaaaa ttgctgtttt cggttcggat atgacaaccg aaattgctca ggagctggaa
    23461 aacaatcagg tgctgaaagc ggtagtggat atttccggta agaaaatggg caatgctgtt
    23521 ttcgcgcaaa cattgaaggt tatcaataaa caagccgacg gtgaaaaagt gattcaggtg
    23581 ccaatcgatc tctataccaa aacggaagat ggtaaacagt ggctggcaac gcacgttgat
    23641 ggtctgccct aatcgctgat tcgcagaggt ccctatgttc acggcaacag aggcagtccc
    23701 ggtagcaaaa gtggtggcag gaaataagcg ttaccccggc gtcgttgcgc tggataacgt
    23761 taacttcacg ctcaataaag gcgaagttcg tgcgctgtta ggtaaaaacg gcgcgggcaa
    23821 atcgactctc attcgaatgc ttaccggcag cgaacgcccg gatagcggtg atatctggat
    23881 tggcgagacg cgactggaag gtgacgaagc tacgttgact cgccgtgccg ctgaactggg
    23941 ggttcgtgcg gtttatcagg aattaagtct ggtggaaggg ctgacggtgg cagaaaacct
    24001 ctgccttggt caatggcctc gccgcaacgg catgattgat tacctgcaaa tggcgcagga
    24061 tgcccaacgt tgcttacagg cgctgggcgt tgacgttagt cctgaacaac ttgtttcaac
    24121 gctaagcccg gcgcaaaagc aactggtgga aattgcacgg gtgatgaagg gcgagccgcg
    24181 cgtggtcatt cttgatgaac ccaccagctc gcttgccagt gcagaagttg aactggtgat
    24241 cagtgcggtg aaaaagatgt cggcactggg cgtggcggtg atttatgtca gccaccggat
    24301 ggaagaaatc cgccgcattg cctcctgtgc caccgttatg cgcgatggtc aggtggcggg
    24361 cgatgtgatg ctcgaaaaca cctccacgca tcatattgtg tcgctaatgc tagggcgtga
    24421 tcacgttgat attgcgccgg ttgcccctca ggaaattgtg gatcaggccg tactggaagt
    24481 ccgtgcgtta cgccataagc ccaagctgga ggatatcagc tttacgttac gtcgcggcga
    24541 agtgcttggc attgctggcc tgctgggggc agggcgcagt gaattgctga aggcgattgt
    24601 tgggctggag acgtatgaac agggcgaaat tgttatcaac ggcgagaaaa tcacgcgccc
    24661 cgattacggc gacatgctga aacgcggcat tggctatacg ccagaaaacc gcaaagaagc
    24721 ggggatcatt ccctggttgg gcgttgacga aaatacagtg ctgaccaatc ggcaaaaaat
    24781 cagcgccaac ggagtgctgc aatggtccac catccgccgc ctgaccgaag aggtgatgca
    24841 gcggatgacg gtcaaggccg ctagtagcga aacgcccatc ggcacgcttt ctggtggcaa
    24901 tcagcaaaaa gtggtgatcg gtcgttgggt ctatgccgcc agccagattt tgttgctcga
    24961 cgagccaacg cgcggcgtcg atatcgaagc caaacagcag atttaccgta ttgtccgtga
    25021 gctggctgcc gaaggaaaaa gcgtggtgtt tatctccagt gaagtggagg agttgccgct
    25081 ggtgtgtgac cgcatcctgt tattacagca cggcacgttc tcgcaggagt ttcactcgcc
    25141 ggtcaatgtg gatgagctga tgtccgccat tctgtctgtg cactgatgaa ttttaccgag
    25201 gaacctgaag atgtctgctt cgtcattacc attgccgcag ggcaagagcg tctcgctcaa
    25261 acaatttgtc agtcgccata ttaatgagat cggtttgctg gtggtgattg ccattcttta
    25321 tctggtcttc tccctgaacg cgcctggctt tatctcattg aataaccaga tgaacgtgct
    25381 gcgcgatgcc gccaccattg ggattgccgc ctgggcgatg acgctgatta ttatctccgg
    25441 tgaaattgat gtcagcgttg ggccgatggt ggcttttgtc tcggtgtgcc tggcattttt
    25501 gctgcaattt gaagttccgt tggcgattgc ttgtctgctg gtgttgctgt taggcgcgct
    25561 gatgggaacg ctcgccgggg tgctgcgcgg cgtgtttaac gtaccaagtt tcgttgccac
    25621 actggggctg tggagcgccc tgcgcggaat ggggctgttt atgaccaacg cactgccagt
    25681 gccgattgac gaaaacgagg tgctggactg gctgggcgga caatttctcg gtgtgccagt
    25741 atccgcgctg atcatgatgg tgttgtttgc gctgtttgtg ttcattagcc gaaaaaccgc
    25801 cttcgggcgc tcggtttttg ccgttggcgg taatgccacg gcggcgcagt tgtgcggtat
    25861 taacgttcgt cgggtgcgca ttcttatctt taccctttcg ggattattag cggcggtgac
    25921 cggcattttg ctggcggcac gcctcggttc cggtaacgca ggtgccgcaa acggtctgga
    25981 gtttgacgtc atcgccgcgg tcgtggtcgg cggtacggca ctttccggcg gtcgcggttc
    26041 cctgttcggt acattgcttg gtgtgctggt gattacgcta atcggtaacg gtctggtgct
    26101 gctcggtatt aactcctttt tccagcaggt ggtacgcggc gtcatcatcg tggtggcggt
    26161 gctggcgaat atcttgctga cccagcgaag cagtaaagcg aaacgctaaa ccttaacgcc
    26221 cctggcgcgg gtgcggcagg gatgaaaatt aatcgtattg ttaatgaggg atttatgaaa
    26281 acgatgctgg cagcttattt accaggaaat tcaaccgtcg atctgcggga agttgcggtg
    26341 ccgacgccgg ggattaacca ggtactgatc aaaatgaaat cctccgggat ttgcggaagc
    26401 gatgtccact atatctatca tcaacaccgt gccacggcgg cggcacccga taaaccgtta
    26461 taccagggat ttatcaacgg tcatgaaccg tgcgggcaga ttgtggcgat ggggcaaggc
    26521 tgccgccatt ttaaagaggg cgaccgcgtg ctggtgtatc acatttctgg ctgtggtttt
    26581 tgcccgaact gccgccgcgg ctttcctatc tcttgtactg gcaaaggaaa agcggcttac
    26641 ggctggcagc gtgacggcgg tcatgccgaa tatctgctgg cggaagaaaa agatctgatc
    26701 ctcctgccgg atgcgctgag ctacgaagat ggtgcgttta tcagttgcgg cgttggcacg
    26761 gcctatgaag ggattttgcg cggcgaagtt tccggcagcg ataacgtgct ggtggtcggt
    26821 ctggggccgg tcggcatgat ggcgatgatg ctggcgaaag gtcgcggtgc aaaacggatc
    26881 atcggcgttg atatgctgcc ggaacgtctg gcgatggcaa aacagttagg ggtgatggat
    26941 cacggctatt tagcaaccac cgaaggtctg ccgcagatta tcgccgaact cacccacggt
    27001 ggcgcggatg ttgcgctcga ttgttctggt aatgccgcag gtcgcttgct ggcactgcaa
    27061 tccaccgctg actggggacg ggtggtttac attggtgaaa ccggaaaagt ggaattcgag
    27121 gtcagcgccg atctgatgca ccatcaacgg cggattatcg gctcctgggt gaccagtctg
    27181 ttccatatgg aaaaatgcgc ccacgatttg actgactgga agctgtggcc gcgtaacgcc
    27241 attacccatc gcttctcgct ggaacaggca ggtgatgcct atgcgctgat ggcgagcggc
    27301 aaatgcggga aagttgtgat taacttcccg gattaaggag ttagcggatg accatctata
    27361 ccttatccca cgggtcgcta aagctggacg tttctgacca gggcggcgta atcgaaggct
    27421 tctggcgcga tacgacgccg ctcttacgtc ctggtaaaaa aagcggtgtg gcaaccgatg
    27481 cctcatgttt tccgctggtg ccatttgcaa accgggtgag cggcaaccgt tttgtctggc
    27541 aggggcgtga gtatcaactg caaccgaatg ttgagtggga tgcgcactat ctacacggcg
    27601 atggctggct gggcgagtgg caatgtgtct cgcacagcga tgatagtttg tgtctggtgt
    27661 atgaacatcg cagcggtgtc tatcactatc gggtaagtca ggcgtttcat ttaacggcgg
    27721 atacgctgac ggtgacgctc tctgtcacca atcaaggggc agagacgctg ccatttggta
    27781 ccggctggca tccttatttt ccgttgtcac cacaaacgcg gattcaggcg caggcgagcg
    27841 gttactggct ggaacgggag cagtggctgg cgggtgagtt ttgcgagcag ctaccgcagg
    27901 aactggattt taaccagctc gcgccgttgc cgcgccagtg ggtgaacaat ggttttgccg
    27961 gatggaatgg tcaggcccgc atcgagcagc cgcaggaagg ttatgccatc atcatggaaa
    28021 cgacgccacc tgcaccgtgt tatttcatct ttgtttccga ccctgcgttt gataaaggat
    28081 atgcgtttga tttcttctgt ctggaaccga tgagccatgc gccggatgat catcatcgcc
    28141 cggaaggggg tgatctcatt gcgctagcac caggggaatc aacaacttca gagatgtcgt
    28201 tgcgggtaga gtggctgtaa tttattaaat gccggataag gcgttcacgc cgcatccggc
    28261 atctttccta acgccgatcg agagaaattg cccccggccc ggtaattgcc aatagcaaga
    28321 acgcgccagc gatactgaca ttcttccaga aattaatcat atttggccca accgcatcgc
    28381 cggtcatatc ccagtaatgg tgaccaatca ccgccgtacc cagcgtgtag aaaataaaca
    28441 gcaccgccag cggacgggtg aaaaagccaa gcacgattaa tatcgcggcg ggcacttcca
    28501 taactaccgc aataatcgct gccagcatcg gcattggcgc gcccaacgag gccatatatt
    28561 ggaccgtacc gtcaaagccc atcattttgg gaaaaccaaa aataatgaaa attaagacca
    28621 cggcgatacg ggcaattaat aacaaaacgg ggcgggcagc tccaaaatca aaataacgt
//
LOCUS       DAJVRF010000040        28662 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJVRF010000040 DAJVRF010000000
VERSION     DAJVRF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28662
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.040"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..849)
                     /gene="melB"
                     /locus_tag="OQN13_001597"
     CDS             complement(<1..849)
                     /gene="melB"
                     /locus_tag="OQN13_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCS1614981.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTL"
     gene            complement(952..2307)
                     /gene="melA"
                     /locus_tag="OQN13_001598"
     CDS             complement(952..2307)
                     /gene="melA"
                     /locus_tag="OQN13_001598"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCS1614982.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYLHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2590..3498
                     /gene="melR"
                     /locus_tag="OQN13_001599"
     CDS             2590..3498
                     /gene="melR"
                     /locus_tag="OQN13_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCS1614983.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3697..5964
                     /gene="adiA"
                     /locus_tag="OQN13_001600"
     CDS             3697..5964
                     /gene="adiA"
                     /locus_tag="OQN13_001600"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCS1614984.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            6289..7050
                     /gene="adiY"
                     /locus_tag="OQN13_001601"
     CDS             6289..7050
                     /gene="adiY"
                     /locus_tag="OQN13_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCS1614985.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            7187..8524
                     /gene="adiC"
                     /locus_tag="OQN13_001602"
     CDS             7187..8524
                     /gene="adiC"
                     /locus_tag="OQN13_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCS1614986.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            8628..10271
                     /gene="eptA"
                     /locus_tag="OQN13_001603"
     CDS             8628..10271
                     /gene="eptA"
                     /locus_tag="OQN13_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCS1614987.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            10268..10936
                     /gene="basR"
                     /locus_tag="OQN13_001604"
     CDS             10268..10936
                     /gene="basR"
                     /locus_tag="OQN13_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCS1614988.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            10937..12037
                     /gene="pmrB"
                     /locus_tag="OQN13_001605"
     CDS             10937..12037
                     /gene="pmrB"
                     /locus_tag="OQN13_001605"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCS1614989.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISIFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(12013..12102)
                     /gene="pmrR"
                     /locus_tag="OQN13_001606"
     CDS             complement(12013..12102)
                     /gene="pmrR"
                     /locus_tag="OQN13_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCS1614990.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(12214..13716)
                     /gene="proP"
                     /locus_tag="OQN13_001607"
     CDS             complement(12214..13716)
                     /gene="proP"
                     /locus_tag="OQN13_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCS1614991.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(13980..14846)
                     /locus_tag="OQN13_001608"
     CDS             complement(13980..14846)
                     /locus_tag="OQN13_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCS1614992.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(14843..17071)
                     /gene="crfC"
                     /locus_tag="OQN13_001609"
     CDS             complement(14843..17071)
                     /gene="crfC"
                     /locus_tag="OQN13_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCS1614993.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGVHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            17484..17819
                     /locus_tag="OQN13_001610"
     CDS             17484..17819
                     /locus_tag="OQN13_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCS1614994.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            17977..18420
                     /gene="yjdN"
                     /locus_tag="OQN13_001611"
                     /pseudo
     CDS             17977..18420
                     /gene="yjdN"
                     /locus_tag="OQN13_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            18553..19341
                     /gene="phnC"
                     /locus_tag="OQN13_001612"
     CDS             18553..19341
                     /gene="phnC"
                     /locus_tag="OQN13_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCS1614995.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVALNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            19366..20382
                     /gene="phnD"
                     /locus_tag="OQN13_001613"
     CDS             19366..20382
                     /gene="phnD"
                     /locus_tag="OQN13_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1614996.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            20508..21287
                     /gene="phnE"
                     /locus_tag="OQN13_001614"
     CDS             20508..21287
                     /gene="phnE"
                     /locus_tag="OQN13_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCS1614997.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            21308..22033
                     /gene="phnF"
                     /locus_tag="OQN13_001615"
     CDS             21308..22033
                     /gene="phnF"
                     /locus_tag="OQN13_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCS1614998.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            22034..22486
                     /gene="phnG"
                     /locus_tag="OQN13_001616"
     CDS             22034..22486
                     /gene="phnG"
                     /locus_tag="OQN13_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCS1614999.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            22483..23067
                     /gene="phnH"
                     /locus_tag="OQN13_001617"
     CDS             22483..23067
                     /gene="phnH"
                     /locus_tag="OQN13_001617"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCS1615000.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            23067..24131
                     /gene="phnI"
                     /locus_tag="OQN13_001618"
     CDS             23067..24131
                     /gene="phnI"
                     /locus_tag="OQN13_001618"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCS1615001.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            24124..24969
                     /gene="phnJ"
                     /locus_tag="OQN13_001619"
     CDS             24124..24969
                     /gene="phnJ"
                     /locus_tag="OQN13_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCS1615002.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            24966..25724
                     /gene="phnK"
                     /locus_tag="OQN13_001620"
     CDS             24966..25724
                     /gene="phnK"
                     /locus_tag="OQN13_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCS1615003.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            25835..26515
                     /gene="phnL"
                     /locus_tag="OQN13_001621"
     CDS             25835..26515
                     /gene="phnL"
                     /locus_tag="OQN13_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCS1615004.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            26512..27648
                     /gene="phnM"
                     /locus_tag="OQN13_001622"
     CDS             26512..27648
                     /gene="phnM"
                     /locus_tag="OQN13_001622"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCS1615005.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            27648..28205
                     /gene="phnN"
                     /locus_tag="OQN13_001623"
     CDS             27648..28205
                     /gene="phnN"
                     /locus_tag="OQN13_001623"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCS1615006.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            28192..28626
                     /gene="phnO"
                     /locus_tag="OQN13_001624"
     CDS             28192..28626
                     /gene="phnO"
                     /locus_tag="OQN13_001624"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCS1615007.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
BASE COUNT         6937 a         7956 c         7398 g         6371 t
ORIGIN      
        1 taacgtcacc aggttagcag ctcccgcata cgacagataa taggggaaca aatccgcatc
       61 accgataaca tatgagaaat aatagatagc aaatccggta ataatgttgc tggctacatt
      121 ataagcaaga gccataccca agaggcatga aagctgatcg tttttataaa ttagcgcaac
      181 gatggcttta agtgtcagat ggcttccttc agcagacggt tgattgtctg acgaaaagac
      241 ttcatgcaca ttgcgcagag tgatgatggt tgaaacaata aaaaaggcga tcagtaccag
      301 tgtgaacatc tgaaagccaa atccccgatc gccaccgccg acataattaa caaatggtag
      361 cgtcacacct gccgtaacaa agcccgccag actggcaaaa aaacgcggat aaggaaccag
      421 ttgttcacgc tcacgtttat cgagcgtgat ggttggaacc agcgaccaga agggaatatc
      481 cataatggtg taagtcatgc cccagaggat gtaggtcacg caaacaaaga caatctgagt
      541 agtaccttca aacagatgcg cactaaagag gagaaataag attacagagt ttgccaacgt
      601 accgatcagg atccagggtt tgaacttacc ccatcgcgat cgcgtagcat ttacaatcca
      661 tcccataatc ggatcgttaa tagcatccca gatcctcgcc accagaaaca aagtaccgac
      721 caaacccaca gacagcccaa cgacatcggt gtagtaatac atgaggtaca tatacacaat
      781 gccgatcgca aaatccttcc cgaacgctcc aaatccataa ctgagttttg tagtcattga
      841 aatgctcata gggtatcggg tcgctgttac caacgcccgc cttctgttat tggtatcgtc
      901 agaggtgtgc gccggaataa atcccccggc gcagtagcgt ttagtcgcgt tttaacggtg
      961 caaccagcct ggcagccagt cgccgtgggc ggcaatcagg tcgtcaacaa gagcatatat
     1021 ttcgtcaatg cccagcacgg cggcagtatg cgggtccatc atcgcggcgt ggtaaacacg
     1081 gtcgcgattt tccgtaagaa tagcttcggt cagcagcgtc tgtacattga tgttggtttg
     1141 catcagggcg gccagatgcg aaggtagcgt accgacttta gtcggctgaa tgccattagc
     1201 atcaaccaga caggctactt ccacgcaaca tccttgtggc aggttatcaa tcaaaccatc
     1261 gttacggacg ttgccgtaaa tcacactcgg ctcgccagtc cagatagcgt tcatgattgt
     1321 gctggcatat tctcgtgacg gtttaatatc aatccgggag gcgtttttat actcctccag
     1381 ctctttatgc cagttcgcca gctgctcgac gcagcgtttc gggtactcat ccagcggtac
     1441 tttataacgc tcaatcaaat cctcacgacc tggcttaata aaccacggcg tgtattcagc
     1501 aaaatgttct gacgattccg taacgaagta gcccagcttt ttgaacattt cgtagcgcac
     1561 aatattctgg cagcgagtat taccatgaat atttggcttc ggtgcctgcc ctgcttcata
     1621 agccgccagc agttccgggt agagattcac gtaactgccg tcggcggttt tacgctccag
     1681 ttccaggtaa aacgccatat ggttgatacc agcgcaacgg taacgcagcg tggacggatc
     1741 gatattgagg tcacgcgcca gctcttccgc cgttccctgc accgaatggc acagccctac
     1801 ctgtttgata tgcagatagc gggcatacat cgcccaggta ttcatcgcca ttgggttaac
     1861 gtagttgagc atggtggcat cggggcagac ttccgtcatg tcctcgcaaa tttgccacag
     1921 atgcggaatg gtacgtagcg cgcgcataat accgcccggc cccaacgtat cggcaatggt
     1981 ttgttccaga ccatgccgct tacagacctc gaaatcagtc accgtgcaag gttcataacc
     2041 gccaatctga aatgccacca cgacaaaatc ggcatcctgt aaggcttctt tctgttgggt
     2101 gtggcaggtg attttgccgc tggcccctgc tgaatccatc agcttacgca ccacaatatg
     2161 cgactcttcc aggcgggtgg gatcaatgtc catcagggca atatgcgccg ttttcagcgc
     2221 ctcgcgatgg aacacatcac caagaatatt tttaacgaaa atcgtcgaac cagcgccgat
     2281 aaatgtaatt ttgggtgcag tcatcatgca gatctcctgg cttgcttgaa taacttcatc
     2341 atggcaggcg aatctgcaga aaacctccgg cttccaggtt gagcattccc ataaactcag
     2401 atcctgcgtg aagcagcagt aaatctgagt ttatggtaat aagttacctg aaaagcagag
     2461 ttttcggcct catgatgctc ggacgctccc ctcgcctctc tgggaagagg gtgaaaaccg
     2521 tgctcccact cgcagtcatc ctccctcact cctgccataa ttctgatatt ccaggaaaga
     2581 gagccatcca tgaatacaga tacgtttatg tgcagcagcg acgaaaaaca gacccgcagc
     2641 ccgctgtcgc tgtattcgga ataccagcgg atggagattg agtttcgcgc accgcatatc
     2701 atgcccacca gccactggca tggtcaagtc gaagtgaatg tgcctttcga tggcgatgtg
     2761 gaatacctga tcaacaatga aaaagtgaat atcaatcagg gtaatatcac gctgttctgg
     2821 gcctgtacac cgcaccaact aacagatacc ggaacctgtc agagcatggc gatttttaat
     2881 ctgccgatgc atctgtttct ctcctggccg ctggataaag acctgattaa ccacgtcact
     2941 cacggcatgg tgatcaaatc actggccaca cagcaactta gcccgtttga agtgcgccgc
     3001 tggcagcagg aattaaacag tccgaacgag caaattcgcc agctcgccat tgatgaaatt
     3061 ggcctgatgc tcaagcgatt tagcctctct ggctgggaac cgattctggt caataaaacc
     3121 tcgcgcacac acaaaaacag cgtctcgcgc catgcgcaat tttatgttag ccagatgctg
     3181 ggctttattg ccgaaaacta tgatcaggcg ctgaccatca acgatgtggc tgagcacgtc
     3241 aaacttaacg ccaactatgc aatggggata ttccagcggg tcatgcaatt gacgatgaaa
     3301 cagtacatta ccgcgatgcg catcaaccac gttcgcgcgt tactgagcga taccgataaa
     3361 agtattctcg atattgccct gacggcaggc tttcgttcaa gtagtcgttt ttacagcacg
     3421 ttcggcaaat atgtcggcat gtcgccgcaa caataccgca aacttagcca acagcgccgc
     3481 cagacgtttc ccggctaaag acgatatcag tatcagccaa aaaaatagtt tagccgtcga
     3541 taataagcag tgaaaataat ttaaaataat cacataacgc attgtttata aaaataaata
     3601 tttcaaaaat gtttgttttt cacgcgcttt acagcccgaa aaggccggaa gatacttgcc
     3661 cgcaacgaag attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc
     3721 gagtttctcc atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta
     3781 agccagcaaa atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc
     3841 tcttcaaacg aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa
     3901 catcaaaacg tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc
     3961 ttcctgttgg gcgatcggga aaaagccctc gccgcaatgg atcgcgacct gctggagctt
     4021 gtcgatgaat tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgctgtt
     4081 gccgcgatga cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa
     4141 tatagcgaca tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt
     4201 accaaaacac ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc
     4261 gatatgggca tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc
     4321 gaaagcgaaa aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc
     4381 ggtacttccg gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg
     4441 gtcgttgacc gtaactgcca taaatccatc gaacaaggct tgatgctgac aggcgcgaaa
     4501 ccggtctaca tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag
     4561 gaaatgcaac ctgaaacctt gcagaagaaa atcagtgaaa gcccgctgac taaagacaaa
     4621 gccgggcaaa aaccgtctta ctgcgtggtg accaactgca cctatgacgg cgtgtgttat
     4681 aacgctaaag aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa
     4741 gcctggtacg gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc
     4801 gaacctggcg atcacaacgg tcctaccgtt ttcgccaccc actccaccca caaactgctg
     4861 aatgcgcttt cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc
     4921 tcccgcttca accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc
     4981 gcatccaacg acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacgcag
     5041 gaagtgatcg acgaagcggt tgatttccgt caggcgatgg cgcggctgta taaagagttc
     5101 accgctgacg gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa
     5161 accggcaaaa cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac
     5221 tgctgggtaa tgcatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg
     5281 agtatgctcg acccgattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa
     5341 ctggaagaaa ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt
     5401 gtgcctaccc gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt
     5461 gggaaatggg gaactctggt taacaccctt tgctccttca aacgccacta tgacgccaac
     5521 acaccgctgg cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac
     5581 atggggattc acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc
     5641 gcacggttga acgaagccta ttccggcctg tcggtggcgg aaatcacccc gcgtgaagcg
     5701 tacaacgcga ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc
     5761 atcgcggcaa actcagttat cccatatccg ccaggaatcc cgatgctgct gtctggtgaa
     5821 aacttcggcg ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac
     5881 caccatttcc ctggatttga acacgaaact gaagggactg aaattattga cggtatttac
     5941 cacgttatgt gtgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac
     6001 attacgccgc ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca
     6061 aacaaaaatg tcatactttt tgcgcggccc caccccgcgc ttttttttgc ctgttattta
     6121 tcctgtaaaa aaatatgtac atgagaaatt actataaaaa tttgtaatat tagtaaaact
     6181 cgttattttt atgcatgttt atattcatca tacaattata taaccatttc ccggtatcgc
     6241 tttgctttag cgagaactgg tcttttattc gcactcagga gtacatgtat gaggatttgc
     6301 agcgaccaac cttgtattgt tttattgact gaaaaagatg tctggataag ggtgaatggg
     6361 aaagaaccta ttagccttaa agctaaccat atggcgttat taaattgtga aaataatatt
     6421 atcgacgtct cctctcttaa caacactttg gttgctcata ttagtcacga catcatcaaa
     6481 gattacctcc ggtttctgaa taaagatctc tcgcaaatac cagtatggca acgtagcgct
     6541 acgcccatac tcaccctgcc atgcctgacg ccagacgtct ttcgcgttgc cgcgcaacac
     6601 agcatgatgc ccgcagaaac tgagtcagaa aaggaacgaa cacgcgcatt attattcacg
     6661 gtgctatccc gttttctcga cagtaaaaaa ttcctttcat taatgatgta tatgttacgt
     6721 aattgtgtaa gtgacagcgt ttatcaaatt attgaaagcg atattcacaa agactggaat
     6781 cttagtatgg tagccagttg tttatgtctt agcccaagtc tgttaaagaa aaagctgaaa
     6841 agcgaaaaca ccagttatag ccaaataatc accacctgcc gcatgcgtta tgccgtaaat
     6901 gaattaatga tggacggtaa aaatatctct caggtatcac agtcctgcgg ctacaacagt
     6961 acgtcgtact ttatttctgt ctttaaagac ttctacggta tgacgccgct gcattatgtt
     7021 agtcagcaca gagaacgaac tgtcgcctga tttttaacct taacgaagag ctatattaat
     7081 aacggcatca gcgataaccc ggtcgataat aattcaacta tcgaatgcag gcgtatgata
     7141 tgacgtaatt tattgtcacg aagctcgcct tcgcaggagt ttaattatgt cttcggatgc
     7201 tgatgctcac aaagtgggct taatccccgt caccctgatg gtgtcgggga atattatggg
     7261 gtcaggtgtt tttctgttac ctgcaaacct ggcctccact ggcgggattg ccatttatgg
     7321 atggttggtg acgattatcg gtgcgctggg gctctcgatg gtatacgcca aaatgtcgtt
     7381 cctcgatcca agtcctggtg gttcttacgc ttacgcccgc cgctgctttg gcccgtttct
     7441 cggttatcaa accaacgtcc tctactggct ggcctgctgg atcggcaata tcgctatggt
     7501 ggtcattggc gtaggatatt taagttactt cttcccgatt ctgaaagatc cattggtatt
     7561 aaccatcacc tgcgtcgtgg tgctgtggat cttcgtcctg ctgaacattg tcggtccgaa
     7621 aatgatcacc cgtgtgcagg ccgttgccac cgtgctggcg ctgatcccca tcgtcgggat
     7681 tgccgtattt ggctggttct ggttccgtgg tgaaacctat atggcggcat ggaacgtcag
     7741 cggcctgggc accttcggtg cgattcaaag tacccttaac gttacgctgt ggtcgttcat
     7801 cggtgtggaa agtgcctccg ttgccgcagg tgtggtgaaa aacccgaaac gcaatgtccc
     7861 tatcgccacc attggtgggg tattgattgc cgccgtttgc tatgtgctct ccaccaccgc
     7921 gattatgggg atgatcccta atgccgcact gcgcgtttct gcctcgccat tcggtgatgc
     7981 cgcacggatg gcgttgggtg acaccgctgg ggccattgtt tccttctgcg cagctgcggg
     8041 ttgcttaggt tcactgggcg gctggacgtt gctggcgggt caaacggcga aagccgctgc
     8101 cgatgacgga ctgttcccac cgatttttgc ccgtgtaaat aaagcgggta cgccagtggc
     8161 ggggctgatt atcgtcggta ttttgatgac catcttccag ctcagtagca tttcgccaaa
     8221 cgcaaccaaa gagttcggtc tggtatcttc cgtgtcggtc atctttacac tggtgccata
     8281 tctttacacc tgtgcggcgt tactgctgct cggccacggt cactttggta aagcacgccc
     8341 ggcatatctg gctgttacta ccattgcctt cctctactgc atctgggccg tggtggggtc
     8401 cggggcgaaa gaggttatgt ggtcatttgt caccctgatg gtcatcaccg ccatgtatgc
     8461 gctgaattac aaccggctac ataaaaaccc gtatccctta gatgcaccaa taagcaaaga
     8521 ttaattcccc ttaatccagc aaacataaaa gccaacctta agaacttaag gttggcttaa
     8581 ttttgctttg cgagcatatg cgcactttgt tcgatggaaa caccgtgatg ttgaagcgcc
     8641 tactaaaaag cccctctttg aatttactcg cctggctatt gttggccgct ttttatatct
     8701 ctatctgcct gaatattgcc ttttttaaac aggtgttgca ggcgctgccg ctggattcgc
     8761 tgcataacgt actggttttc ttgtcgatgc cggtcgtcgc tttcagcgtg attaatattg
     8821 tcctgacact aagctctttc ttatggctta atcgaccact ggcctgcctg tttattctgg
     8881 ttggcgcggc tgcacaatat ttcataatga cttacggcat cgtcatcgac cgctcgatga
     8941 ttgccaatat tattgatacc actccagcag aaagttatgc gctgatgaca ccgcaaatgt
     9001 tattaacgct gggattcagc ggcgtgcttg ctgcgctgat tgcctgctgg ctaaaaatca
     9061 aacctgccac ctcgcgtctg cgcagtgttc ttttccgtgg agccaatatt ctggtttctg
     9121 tactgctgat tttgctggtc gccgcactgt tttataaaga ctacgcctcg ttgttccgca
     9181 ataacaaaga gctggtgaaa tccttaagcc cctctaacag cattgttgcc agctggtcat
     9241 ggtactccca tcagcgactg gcaaatctac cgctggtgcg aattggtgaa gacgcccacc
     9301 gcaacccgtt aatgcagaac gaaaaacgta aaaatttgac catcctgatt gtcggcgaaa
     9361 cctcgcgggc ggagaacttc tccctcaacg gctacccgcg tgaaactaac ccgcggctgg
     9421 cgaaagataa cgtggtctat ttccctaata ccgcatcttg cggcacggca acggcagttt
     9481 cagtaccgtg catgttctcg gatatgccgc gtgagcatta caaagaagag ctggcacagc
     9541 accaggaagg cgtgctggat atcattcagc aagcgggcat caacgtgctg tggaatgaca
     9601 acgatggcgg ctgtaaaggt gcctgcgacc gcgtgcctca ccagaacgtc accgcgctga
     9661 atctgcctgg tcagtgcatc aacggcgaat gctatgacga agtgctgttc cacgggctgg
     9721 aagagtacat caataacctg cagggtgatg gcgtgattgt cttacacacc atcggcagcc
     9781 acggtccgac ctattacaac cgctatccgc cgcagttcag gaaatttacc ccaacctgcg
     9841 acaccaatga gatccagacc tgtaccaaag agcaactggt gaacacttac gacaacacgc
     9901 tggtttacgt cgactatatt gttgataaag cgattaatct gctgaaagaa catcaggata
     9961 aatttaccac cagcctggtt tatctttctg accacggtga atcgttaggt gaaaatggca
    10021 tctatctgca cggtctgcct tatgccatcg ccccggatag ccaaaaacag gtgccgatgc
    10081 tgctgtggct gtcggaggat tatcaaaaac ggtatcaggt tgaccagaac tgcctgcaaa
    10141 aacaggcgca aacgcaacac tattcacaag acaatttatt ctccacgcta ttgggattaa
    10201 ctggcgttga gacgaagtat taccaggctg cggatgatgt tctgcaaact tgcaggagag
    10261 tgagtgaatg aaaattctga ttgttgaaga cgatacgctg ttattgcagg gactgattct
    10321 ggcggcgcaa accgaaggct acacgtgcga tggcgtaaca accgcgcgga tggcggaaca
    10381 aagccttgag gccggtcatt acagcctggt ggtactggat ttagggttac ccgacgaaga
    10441 tggactgcat tttctcgccc gtatccggca gaaaaaatac accctgccgg tactgatcct
    10501 caccgctcgc gatacgctga ccgacaaaat cgccgggctg gatgtcggtg ccgatgacta
    10561 tctggtgaag ccttttgcgc tggaagagtt acacgcccgt atccgcgccc tgctacgacg
    10621 ccataataat cagggcgaaa gtgagctgat tgttggcaat ctgacgctga atatgggtcg
    10681 ccgtcaggta tggatgggcg gtgaagagtt gattctgacg cccaaagaat atgctctgct
    10741 gtcacggtta atgctcaaag caggcagtcc ggtacatcgg gaaattctct acaacgacat
    10801 ctataactgg gacaatgaac cctcgaccaa caccctggaa gtgcatatcc acaatctgcg
    10861 cgacaaagtg ggcaaagccc gtatccgcac cgtgcgcggc tttggctata tgctggtcgc
    10921 gaatgaggaa aactaattga atctgatgcg ttttctgcgc cgaccaatat cgctgcgcca
    10981 acggctgata ttgaccatcg gggccatttt gttggtgttt gagctgatca gcatcttctg
    11041 gctatggcat gaaagtaccg agcagattca gctgtttgag caggcgctac gcgacaatcg
    11101 caacaacgat cgccacatca tgcgtgagat ccgcgaggcg gtcgccagcc tgattgtccc
    11161 cggcgtcttt atggtcagcc tgacgctatt tatctgctat caggcggtac gccgcatcac
    11221 ccgcccgctg gcggagctgc aaaaagagct ggaagcgcgc accgccgaca acttaacgcc
    11281 cattgccatt cacagcgcca ccctcgaaat cgaagcggtg gtttcggcgt tgaacgatct
    11341 ggtcagtcgc ctgacgagca cgctggataa cgaaaggtta tttaccgctg acgtcgcgca
    11401 cgaactgcga acgccactgg cgggggtgcg tttgcatctg gaactgctgg cgaaaacgca
    11461 tcacattgat gtagcaccgt tagtggcacg gcttgatcag atgatggaga gcgtctccca
    11521 gctgctgcaa ctggcgcgtg ccggacagtc attttcttcc ggtaattatc aacatgtaaa
    11581 actgctggaa gatgtcattc tcccctcgta tgacgaactc agtaccatgc tcgaccagcg
    11641 acagcaaacc ctgctattgc cagagagcgc cgccgacatc accgttcagg gtgatgcaac
    11701 cctgctgcgg atgttattgc gaaacctggt agaaaacgcc catcgttaca gcccacaagg
    11761 cagcaacatt atgattaagc tgcaagaaga tggcggagcg gtcatggccg ttgaagatga
    11821 aggaccgggt attgatgaga gtaaatgcgg ggagttgagt aaagcgtttg tacgtatgga
    11881 cagccgttat ggcgggattg gtctggggtt aagtattgtc agccgcatta cacagttgca
    11941 tcacgggcag tttttcctgc aaaaccggca agagacttcc ggcacgcggg cctgggtacg
    12001 gctgaagaaa gatcagtacg tagcaaacca gatataaaga aagctgctga ccaccagcac
    12061 actgaacacg gtagttaaac tttcataaac acggtttttc attgcactct cctccgttaa
    12121 cctggaggag agtatgcgcg tcagagatta agtgaacctt aagagttcaa ttggcctgat
    12181 aagacagcgt cacatcaggc catccgtttc agcttattca tcaattcgcg gatgttgctg
    12241 caccaggcgg gtacgtttcg cctgcaaatc ggcaatctcg tggtcaatat catcgatttt
    12301 ctgctcgata ttatcgtaat gctcgacgag aatttccttc gcttcctgta tatctgacgc
    12361 cgccggtgtc gcacctttca acggacgatt tgccgtctct ttcatggtta cgccggtgat
    12421 taaaccaatc accgccacta ccatcaggta ataggcaggc atcatcagat tctgcgagct
    12481 ttcgaccagc caggccgcca gtgttggcgt cagaccggca accagcaccg aaatattaaa
    12541 tgccgccgcc agcgcgctgt aacggatatg cgtcgggaac atcgctggca aggtagaagc
    12601 cataacgccc gtaaagcagt taaggatcac cgccagcatc agtaacccgg caaaaatcag
    12661 gccgatgacg ttactgttaa tcagaataaa cgccgggatc gccaacacaa acagggcaac
    12721 actaccaagt agcacaaacg gacgacggcc aaaacggtca ctcagcaagc ccatcaccgg
    12781 ctggacaaac agcataccga tcataatggc gataataatc agcaccccgt ggtcttcgga
    12841 gtaatgcagg ttatgcgaca aataactcgg catataggtc agcaacatgt agtaagtcac
    12901 gttggtggca attaccagac caatacatgt caacaggctg cgccagtatt tagtggcaat
    12961 ctctttaaac gagactttcg ggccatcctg caaaccttcg cggtcgccct gttccagttt
    13021 atcaacatgc tgctggaacg ccggagtttc ttccagcgca tggcgcaggt aaagcccgat
    13081 aatccctaac ggcagagcaa taaagaacgg aatacgccag ccccagtcga ggaagttcgc
    13141 ttcgccgaca atggtcgaaa ttaacaccac cacgcccgca cccagcacaa acccggcaat
    13201 agaaccgaag tccagccagc tgcccataaa gccacgttta cggtcagggg agtattccgc
    13261 aacaaatatc gacgccccgg tatattcacc gccgaccgag aaaccttgtg ccatcttaca
    13321 gatcaacagc agaatcggtg cccaaatacc aatcgtgtcg taggacggta ttaagccaat
    13381 acagaacgta ctgatcgaca taatcacaat agtgatagcg aggatcttct ggcgaccata
    13441 tttatcgccc aacataccaa agaagagccc gccaagcggt cgaatcagaa agggaacgga
    13501 gaaagtggca agtgcagcaa ccatctgcac gctggggtca gcccccggga aaaaaacttt
    13561 acctaatgcg taagcaacaa aaccataaac accaaaatcg aaccattcca ttgcattacc
    13621 cagtgatgct gcggtaatgg ctttacgcag tttaccgtca tcaataatgg tgacatcacg
    13681 aagggtaatc ggttttactt ttttcctttt cagcatagct ttcctcgcag agttggccca
    13741 tttccgcaaa cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg gaattgcagg
    13801 atgcaatctc tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa ggaatgtaca
    13861 atcaaaccag ttgacgcctg aattgggcag ttaatgacct ttttacccta ccagtgttta
    13921 aatttgtgat caacttcaca catggatttc gtttaggact cattgatgta actgattatt
    13981 tacgccaaat ttacagagaa gatttcctcg accattgccc ccaccaaacg ctgcgctgaa
    14041 cagagtcgtg gcatccccag cgcgactgtt tgccagcgtt gggtcactga ccagcaaacg
    14101 ggctgtttat ctgccgtgga ccactttccc agccaactta aaaaatcagc atgctcccct
    14161 cccgaagagg caaaccactg atggtaatag tgacgcgtgg tcggtgccgc attgatactc
    14221 tctgccagat gttgtgtcgc tttctcgcgc aaatcaccca gtaacaacac caccgcttgc
    14281 gggcttccca gacggcaggc atcgccaaat gcaccccagc gcaactcctc cagaacgata
    14341 aaacagcgcc caggcaacga ccaggcgtta tacgccccag cacgccagcg ggtaaaaatt
    14401 tgctcgctct gatcacctgc ctgcaccagt aggcctcgtt gcgttaacgc cttttccttc
    14461 cagcgacaac gggctgaaaa ccactcatac aaggcggcaa tttgctgggg gagctccggg
    14521 aaagcagagt gctggcggtg atccattttt tgcagcagcg ccagatgctg cgcgatctgc
    14581 gtcagcgcca gatgaccggg atccagcatc gacgccagtt tttccaccat tgtcagtcgc
    14641 aggctaagcg gcgcatttaa catgccgccg ttcgtccatg tctgaagctg cacacgcgaa
    14701 agaagttcct gcatcagtcg ttcacgaaac tgctgctgat gggcttgtgg aaggcgggca
    14761 tcatcgccct gcgccagatc cactaaaaag cggggataaa ccgactccag cacgcgaccg
    14821 gggccgtcca gtaacgtctt ggtcaatatc gttctgccgt gaaaagtgtt tgaatatcat
    14881 cgcgtaacag ctgggcgtcg gtgtaaatcc agccgtgagt catcacagtc tgctgcaatt
    14941 gctgctgcat cagcctgacc accgattcat tttgttgacg cagagccagg ctttcgcgta
    15001 aacgcgtctg taattccgtc aaacatgaag cgaactcagc gcaaaaagta ttcatgcctg
    15061 ccgtaacaga ttcatcgacc tgctctgcca gaactttagc catttgttgg caataaagat
    15121 cgacttctgc gcttaaagct cgttgcaaca cactgtaatc aaccgtctct gtcggggatt
    15181 tctcatttcc ccgtccccag tcgggctgat tcaaccagcg cgaaaaagtc tcacgcacaa
    15241 cgcctaaacg cgtgctctgc tcgtccgttg catcctggcg cgaaatgact tcactgaaca
    15301 gctggcgagt gttgacgtgg ggaactactc cgtgaaaaac aggaaaatga accccaggac
    15361 gaaaccctga ctcgctcaat tccattttga cttgttgctc aatgggccga ataacatcgg
    15421 ttaacacccg gcaaagggtg gattcaagct cggcaaaacg cagcgtaaag tcgcgactga
    15481 tggtgttctg cgccgtctgt aacagtgtct cacagcaggt acgaatctcg cttaacggct
    15541 ccgaatcatc ctgaaacaag gcggctaact gcgcattcag cgcatcttgc tgttgacgca
    15601 gaaagtggtt ggcggaggtc agggccagct cgatttcatg tttaatctcg ccgctcacct
    15661 gcgcctgatt gagttgcaat agctgcaaac tttcttcgac ctgatggata ttttgccgca
    15721 attgttcaca agcgacgttt aacccgtgcg cacgaaaatc caggtattcc cgcgcctgct
    15781 gcgcgtaatt caacagttta tgcgcagcag atcgcagagc atacaacgag gcgttagcgt
    15841 aagcggcatg aagcaacgcc tgaattggct gggcgaacag cgaatcttcc cacaactgat
    15901 cggcagcatg acgaatatgt tcgaggtccg ccagatcggc atgacgccag cgcctgccga
    15961 gcgcggcatg ggcaaaatct tccacccagc gttgttgctc tggcgctggt aacttaccgt
    16021 tgttggctaa ctcatggcgc gctcgatttg ccaagtagcc ccacatcgac gacaccggaa
    16081 atatctgctg tggcgtaata cagcctttca tcagcgtccc ggaaatcagt gcccgcacct
    16141 ggtcggcgtc gtcactgtta cgatcctgtt gatcgaactt attgaccagc acatacagtg
    16201 gcaccgattg ccccaccgcc aaaatcgcct cacggacctc ttcatcggag atcgatttca
    16261 gttgcgtata atccagcacc gccagtaccg ccgaggcgcg tgccagctgc tggttaagca
    16321 ttttttgcag atgcggttgc ccggcttcat ttggcccggg ggtatccagt aacgtcaact
    16381 gaccgggata actctccagc cccgccagat ggacaaactc cacttcaatc acgggaatat
    16441 gctcaatggc ggcgtaagca gaaaaaggaa aatcgacgtc cagcgccttc gccagtcgca
    16501 ctaaatcatt caaacttttc agacaatgaa aaataggctg ggcacccaga taatattttt
    16561 cgaaagcgac gccattttcg atccgctgca taagcgcacg catatcttta tctatttcca
    16621 gcacatcggt cagatgctta atatcgcaat cacgcaggcg ctgttgtaat tgttgaatta
    16681 aacaatcgat tggcgcgaca tgtgaaaaat gcagtaccgg ttccttttgc ccgggcgtat
    16741 ggcgaataag cgtcggcagc gcagtcattg ggcgattacg attaggcaga acctccgtac
    16801 caacaatggc attaatggtg gttgatttcc ctgctttcat agtaccgaca atcgccagca
    16861 ccatttccag tcgggaaatt ttactcaact cattattcag catcgcgtga cgttcggcga
    16921 tattaggctg actccagggt aaagccagtt gtggcgcgtc gtctccgggt acagagagag
    16981 gcattttttc cagtaactgc aactgttggc gagaaagctg taacaggcgt tcagcctcct
    17041 gacttaactc atacagggtc tgtgtgtaca tagaaaattc ttccttaaag caaattttgt
    17101 tacttttatt tagccagatt gtttttgagt tctgtttgag ttctgttttt ggcttttata
    17161 attactgcaa gaaataattt tatatttagt gtgttgtttt ttatcagaat aaataacgtc
    17221 ttctgatgcg tttaaaacgt cagaaagata aaaatatcat gtgaataaaa aaaagaacaa
    17281 gtagagcatt aacattatct taaataataa atagaggcaa aaagattatt ttctttttgc
    17341 gtttcctttc aaatgaaaac gatcgtcgtc taaaatcagc agtacccccg acaaactcag
    17401 ggattttgtg tataattgcg gcctttttcg gcaatctgcc gttttttggc gcttttgccc
    17461 tgctgacttt tgaggaaatc cacatgtcat taccacactg cccaaaatgc aactccgaat
    17521 acacttacga agataacggc atgtacatct gcccggaatg tgcctacgaa tggaacgacg
    17581 cagaacctgc acaggaaagc gacgagctga tcgttaaaga tgctaacggc aatctgctgg
    17641 ctgacggcga cagcgttacc atcattaaag atctgaaggt gaaaggtagc tcttcgatgc
    17701 tgaaaattgg caccaaagtg aaaaacatcc gcctggttga aggcgaccat aacatcgatt
    17761 gcaaaatcga cggttttggt ccgatgaaac tgaaatctga gtttgtgaaa aagaactgat
    17821 tgtattgtga tcggtaagcc ggataaggcg ctcgcgccgc atccggcaac gatgccgact
    17881 gcctgatgcg acgcttgcgc gtcttatcag gcagcctctc atctgtataa atttcgaact
    17941 acacttaatt ggcttctctt aactgaggtc accatcatgc cgttaagtcc ctacctctct
    18001 tttgccggta actgtgccga cgcaattgct tattatcaac gtacgttggg cgctgaactg
    18061 ctctataaaa tcagcttcgg cgaaatgcca aaatcagcgc aggacagcgc cgagaactgc
    18121 ccttccggaa tgcaatttcc cgataccgcc atcgcccacg ccaatgtgcg cattgccgga
    18181 agcgacatca tgatgagcga tgccatgccg tcaggaaaag ccagctactc cggctttacg
    18241 ctggtgctcg attcgcaaca ggtcgaagaa ggaaaacgct ggtttgacaa tcttgccgct
    18301 aacggaaaaa tcgaaatggc ctggcaggaa actttctagg cgcatggctt tggcaaagtc
    18361 accgataaat ttggcgtacc gtggatgatt aatgtcgtca aacaacaacc aacgcaataa
    18421 cccgccggga ggcccgccct cccgcactgt catcgaattc ccgttaactc ttcatctgtt
    18481 agtcactttt aattaaccaa atcgtcacaa taatccgcca cgatggagcc acttttttag
    18541 ggaggctgca tcatgcaaac gattatccgt gtcgagaagc tcgccaaaac cttcaatcag
    18601 catcaggcgc tgcatgcggt tgctctgaac attcatcacg gtgaaatggt ggctctgctt
    18661 gggccgtcgg gttccggcaa atccaccctt ttacgtcact taagcggttt gattaccggc
    18721 gataaatccg ccggcagcca tatcgagctg ctgggccgca cagtccagcg cgaaggccgt
    18781 ctggcgcgcg atatccgcaa aagccgcgcc aacaccggct acatcttcca acaattcaac
    18841 ctggtgaacc gcctgagcgt actggagaac gtgctgattg gcgcgctcgg cagcacgccg
    18901 ttctggcgca cctgttttag ctggtttacc cgcgagcaga aacaacgcgc gttacaggcg
    18961 ctgacccgcg ttggcatggt gcattttgcc catcaacgcg tttccaccct ctccggcgga
    19021 cagcagcagc gtgtggcgat tgcccgcgcg ctgatgcagc aggcgaaggt gattctggcc
    19081 gatgaaccca tcgcctcgct ggacccggaa tccgcccgca tcgtgatgga caccctgcgc
    19141 gacatcaatc agaacgacgg catcaccgtg gtcgtcacgc tgcatcaggt ggattacgcc
    19201 ctgcgctact gcgaacgcat cgtcgccctg cgccaggggc acgttttcta cgacggcagc
    19261 agccaacagt ttgataacga acgttttgac catctctacc gcagcattaa tcgcatcgaa
    19321 gagaacgcga aagctgcctg acatccccat cattgaggaa aacgaatgaa cgctaagata
    19381 attgcctcgc tggccttcac cagcatgttc agcctcagca ccctgttaag cccggcacac
    19441 gccgaagagc aggaaaaggc gctgaatttc ggcattattt caacggaatc acagcaaaac
    19501 ctgaaaccgc aatggacgcc attcttacag gatatggaga agaagctggg cgtgaaggtg
    19561 aacgccttct ttgccccaga ctacgcaggc attatccagg gaatgcgctt caataaagtg
    19621 gatatcgcct ggtacggcaa cctgtcggca atggaagcgg tggatcgcgc caacggccag
    19681 gtcttcgccc agacggtcgc ggcggatgga tcgccaggtt actggagcgt gttgatcgtc
    19741 aacaaagata gtccgatcaa caacctgaac gatctgctgg cgaagcggaa agatctcacc
    19801 ttcggcaatg gcgatcctaa ctccacctct ggcttcctcg tccccggtta ctacgtcttt
    19861 gccaaaaaca atatctccgc cagcgacttt aagcgcaccg tcaacgccgg gcatgaaacc
    19921 aacgcgctgg ccgtcgccaa caagcaggtg gatgttgcca ccaacaacac cgaaaacctc
    19981 gacaagctga aaacctccgc gccagagaag ctgaaagaac tgaaggtgat ctggaagtcg
    20041 ccgctgatcc caggcgatcc gatcgtctgg cgcaagaatc tctccgaaac caccaaagac
    20101 aagatctacg acttctttat gaactacgga aaaacgccgg aagagaaagc ggtgctggaa
    20161 cgcctgggct gggcaccgtt tcgcgcctcc agcgacctgc aactggtgcc gattcgccag
    20221 ctcgcactgt ttaaagagat tcagggcgta aaaagcaata acggactgaa tgagcaggac
    20281 aagctggcga aaaccaccga gattcaggcg cagctggatg acctggaccg cctgaacaac
    20341 gcgttaagcg cgatgagttc ggtgagtaaa gcggtgcagt aatgtgctcg atcggtcccc
    20401 tctcccttga gggagagggt tagggtgagg gggaaatgcg cacgctgatg ccctcacccc
    20461 ggccctctcc cacagggaga gggagaaaac attaaacgga gccaaacatg caaaccatca
    20521 ccatcgcccc acccaagcgc agctggttct cgcttctgag ctgggccatt gttctcgccg
    20581 tgttggtcgt ctcgtggcag ggcgcggaaa tggccccgct tacgctgatc aaagacggcg
    20641 gcaacatggc gaccttcgcc gccgacttct tcccgcccga tttcagccag tggcaggatt
    20701 acctcaccga aatggccgtc acgctgcaaa tcgccgtctg gggcaccgcg ctggcagtgg
    20761 ttctctccat ccccttcggc ctgatgagcg ccgaaaacct ggtgccgtgg tgggtttacc
    20821 agcccgttcg ccgcctgatg gacgcctgcc gcgccattaa cgaaatggtc ttcgccatgc
    20881 tgttcgtggt cgccgtcggt ctcggaccgt tcgctggcgt gctggcgcta tttattcaca
    20941 ccaccggcgt gctctcgaaa ctgctttccg aagcggtgga agcgattgaa cccggcccgg
    21001 tggaaggcat tcgcgccacc ggcgccaaca agctggaaga gatcctttac ggcgtgctgc
    21061 cacaggtaat gccgctgctg atctcctact ccctctatcg cttcgaatcc aacgtccgct
    21121 cggcgaccgt cgtcggcatg gtcggtgcag gcgggatcgg cgtcaccctg tgggaagcga
    21181 ttcgcggttt ccagttccaa caaacctgcg ccctgatggt gcttatcatc gtcacggtca
    21241 gcctgctgga tttcctctct caacggttgc gtaagcactt tatctgataa gtgaggcatt
    21301 gatatctatg cacttgtcta cacatccgac cagctaccca acacgctatc aggagatagc
    21361 cgcaaaactt gagcaggagc ttcgtcaaca ctatcgctgc ggcgactatc ttcccgccga
    21421 gcagcaactg gcggcgcgct ttgaggtaaa ccgccacacc ctgcgccgcg ccatcgacca
    21481 actggtggaa aaaggctggg tacagcgccg tcagggcatc ggcgtgctgg tgctgatgcg
    21541 cccgttcgat tacccgctca acgcccaggc gcgttttagc cagaacctgc tggaccaggg
    21601 cagccatccc accagcgaaa aactgctgtc ggtattgcgc cccgcgtccg gtcacgttgc
    21661 tgacgcgctg gggattaccg agggtgagaa cgttatccat ttgcgcaccc tgcgtcgcgt
    21721 caacggcgtc gcgctctgtt taatcgacca ctactttgcg gacctcaccc tctggccgac
    21781 actgcaacgt ttcgacagcg gttcgctgca cgattttctg cgcgagcaaa ccggaattgc
    21841 gctgcgccgc agccagacgc ggatcagcgc ccgccgcgcc caggccaaag agtgccagcg
    21901 ccttgaaatc ccgaatatgt cgccgctgct gtgcgtgcgc acccttaacc accgtgacgg
    21961 tgaaagcagc ccggcggagt actccgtcag cctgacgcgc gccgacatga ttgaattcac
    22021 tatggagcac tgaatgcacg cagataccgc gacccgccag cactggatgt ccgtactggc
    22081 gcacagccaa ccggctgaac tggcagcccg cctgaacgcg ctgaacatca ccgccgacta
    22141 tgaagtgatc cgcactgccg aaactggcct ggtgcagatt caggcgcgaa tgggcggtac
    22201 cggcgaacgc ttttttgccg gcgacgccac gctgacccgt gccgccgtac gcctgactga
    22261 cggcaccctc ggctacagct gggtgctggg gcgtgataaa cagcacgccg aacgctgcgc
    22321 gctgattgat gcgctgatgc agcaatctcg ccactttcaa aacttatcag aaacccttat
    22381 tgccccgctg gacgctgacc gtatggcacg cattgccgca cgccaggccg aagtgaacgc
    22441 cagccgggtc gacttcttta cgatggttcg cggagacaac gcatgaccct ggaaaccgct
    22501 tttatgcttc ccgtgcagga tgcccagcac agttttcgtc gcctgttaaa ggccatgagc
    22561 gagccgggcg tgattgtcgc cctgtatcag ctcaaacgcg gctggcagcc gctgaatatc
    22621 gccaccacca gcgtgctgct gacgctggcc gataacgaca cgccggtgtg gcttgccacg
    22681 ccattaagta acgatatcgt caatcagagc ctgcgttttc ataccaacgc gccgctggtc
    22741 agccagccgg aacaggcgac cttcgcggtg acggatgagg cgatttccag cgaacagctc
    22801 aacgcccttt ccaccgacac cgccgttgcg ccggaagcgg gcgcgacgct gattttacag
    22861 gtcgccagcc tgagcggcgg acgcatgttg cgtctcaccg gcgcgggcat tgccgaagaa
    22921 cgaatgatcg ctccgcagct gccagagtgc attctgcacg aactcaccga gcgcccgcac
    22981 ccgttcccgc tcggcatcga cctaatcctg acctgcggcg agcgcctgct ggctatcccg
    23041 cgaaccactc atgtggaggt gtgctgatgt acgttgccgt gaaagggggc gaaaaggcga
    23101 tcgacgccgc ccacgccctg caagagagcc gacgccgggg cgataccgat ttgcccgaac
    23161 tgagcgtcgc ccagattgaa cagcagctta acctcgcggt ggatcgcgtg atgacagaag
    23221 gcggcattgc cgaccgcgaa ctggcggcgc tggcgctgaa acaggccagc ggcgataacg
    23281 tcgaagcgat tttcctgcta cgcgcctacc gcaccacgct gacgaaactg gcggtaagcg
    23341 agccgctcga caccacggag atgcgtctcg aacggcgtat ttcagcggtt tataaagata
    23401 ttcccggcgg ccagctgctt ggcccaacct acgactacac ccatcgcctg ctcgatttca
    23461 ctctgctggc aaacggcgaa gcgccgacgc tgaccaccgc cgacagcgaa caacagccgt
    23521 cgccgcacgt tttcagcctg ctggcgcgtc aggggctggc gaagtttgaa gaggatagcg
    23581 gagcacagcc ggatgacatc acccgcacgc cgccggttta cccctgttca cgttcttccc
    23641 gtttgcagca gttaatgcgc ggcgacgaag gctatttgct ggcgctggct tactccaccc
    23701 agcgcggtta cgggcgcaac cacccgttcg cgggcgagat ccgcagtggt tacatcgacg
    23761 tgtcgattgt gccggaagag ctgggatttg cggtaaacgt cggcgaactg ctgatgaccg
    23821 agtgtgaaat ggtcaacggt tttatcgacc cgccggatga gccgccgcac ttcacgcgcg
    23881 gctacgggct ggtattcggc atgagcgagc gcaaagcgat ggcaatggcg ctggttgacc
    23941 gtgcgttgca agccccggaa tacggcgagc acgcgacagg cccggcgcag gatgaagagt
    24001 tcgtgctggc gcatgccgac aacgtcgaag ccgcaggctt tgtctcgcac ctcaaactcc
    24061 cgcactacgt cgatttccag gccgaactgg agctactcaa acgtctgcaa caggagcaga
    24121 accatggcta atctgagcgg ctacaacttt gcctacctcg acgagcagac caaacgcatg
    24181 atccgccgcg ccatcttaaa agcggtggcg atccccggtt atcaggtacc gtttggcggg
    24241 cgcgagatgc cgatgccgta cggctgggga actggtggta tccagctcac cgccagcgtg
    24301 attggcgaaa gcgacgtgtt gaaggtgatt gaccagggtg cggatgacac caccaacgcc
    24361 gtgtcgattc gcaacttctt taagcgcgtg accggggtaa acaccactga atgtacggac
    24421 gatgcgacgc ttatccagac gcgtcaccgc atccccgaaa cgccgctgac cgaagatcag
    24481 atcattatct tccaggtgcc aatcccggaa ccgctgcgct ttatcgagcc gcgcgaaacg
    24541 gagacccgca ctatgcacgc gctggaggag tacggcgtga tgcaggtgaa actgtatgaa
    24601 gatatcgccc gcttcggtca tatcgccacc acctatgctt atccggtgaa ggtgaacggg
    24661 cgctacgtga tggacccgtc gccgatcccg aaattcgata acccgaaaat ggacatgatg
    24721 cccgccctgc agctgttcgg cgcagggcgc gagaagcgca tctatgcggt gccgccgttt
    24781 acccgcgtgg aaagtctcga tttcgacgat cacccgttca ccgttcagca gtgggatgag
    24841 ccatgcgcca tctgcggatc gacccacagc tatcttgatg aagtggtgct ggatgacgcc
    24901 ggaaaccgca tgtttgtctg ctccgatacc gattattgcc gccaacagag cgaggcgaaa
    24961 agccaatgaa tcaaccgtta ctttcggtca ataacctgac ccacctttac gcgccgggca
    25021 aaggctttag cgatgtctct tttgatttat ggccggggga agtgctgggc attgtcgggg
    25081 aatccggctc cgggaagacc acgctgctga agtcgatctc cgcgcgtctg acgccgcagc
    25141 agggggagat tcactatgaa aaccgttccc tctatgcaat gagcgaggcc gaccgccgtc
    25201 gcctgctgcg caccgaatgg ggcgtggtgc atcagcatcc gctcgacggc ctgcgccgcc
    25261 aggtgtcggc aggcggcaat atcggcgagc ggctgatggc gaccggggcg cgacattacg
    25321 gcgatattcg cgctaccgcg cagaagtggc tggaagaggt ggagattccc gccaaccgta
    25381 tcgacgacct gccgaccacc ttttccggcg gtatgcagca gcgtttgcag attgcccgca
    25441 acctggtgac gcatccgaag ctggtgttta tggatgaacc gaccggcggg ctggatgtgt
    25501 cggtgcaggc ccgcctgctc gacctgctgc gcggactggt ggtggagctg aacctcgcgg
    25561 tagtgattgt cacccacgat ttaggcgtcg cccgcctgct ggcggaccgt ttgctggtga
    25621 tgaagcaggg gcaagtggtg gagagtgggt taaccgaccg cgtgctcgac gacccgcatc
    25681 atccgtatac acagctgctg gtgtcatcgg ttttgcagaa ttgagccggt gccggatgcg
    25741 gcgtaaacgc cttatccggc ctacaaatgc gctccccgta ggtcggataa gacgcgtcag
    25801 cgtcgcatcc gacacccgaa ccacgaggcg aaaaatgatt aacgtacaaa acgtcagtaa
    25861 aaccttcatc ctgcaccagc aaaacggcgt gcgcctgccc gtcctcaacc gcgcctcgct
    25921 catcgtcaac gcgggcgaat gcgtggtgct ccacggccat tccggcagcg gaaaatcaac
    25981 cctgctacgc tcgctgtacg ccaactatct gcccgacgaa gggcaaatcc agatcaaaca
    26041 cggtgacgaa tgggtcgatc tggtcaccgc gccagcgcgc aaagtggtgg agatccgcaa
    26101 aaccaccgtt ggctgggtga gccagtttct gcgcgtcatc ccgcgtattt cggcgctgga
    26161 agtggtgatg cagccgctac tcgataccgg cgtaccgcgt gaagcctgcg ccgctaaagc
    26221 cgcgcgtctg ctcacccgcc tgaacgtgcc ggaacgcctg tggcatctgg cgccatcgac
    26281 cttctccggc ggcgaacagc agcgcgtcaa catcgcccgc ggctttatcg tcgactaccc
    26341 cattctgctg ctcgacgaac ctaccgcctc gctggacgcc aaaaacagcg ccgccgtagt
    26401 cgagctgatt cacgaagcca aagcccgtgg cgcggccatc gtcggcatct tccacgacga
    26461 agccgtacgt aatgacgttg ccgaccgcct gcacccaatg gaagcctctt catgattatc
    26521 aataacgtca aattggtgct ggaaaacgaa gtggtacacg gttcactgga ggtgcaggac
    26581 ggcgaaatcc gcgcctttgc cgaaagccag agccgcctgc cggaggcgat ggacggcgaa
    26641 ggcggctggc tgctgccggg gctgattgag ctgcataccg ataatctgga taaattcttc
    26701 accccgcgcc cgaaagttga ctggcctgcc cactcggcga tgagcagcca cgacgcgctg
    26761 atggtggcga gcggcatcac caccgtactg gatgccgtgg caattggcga cgtgcgcgac
    26821 ggcggcgatc ggctggtgaa tctggagaag atgatcaacg ccatcgaaga gacgcagaaa
    26881 cgcggcgtca accgcgccga gcaccgtctg catctgcgct gcgaactgcc gcatcacacc
    26941 acgctgccgc tgtttgaaaa actggtgcag cgcgagccgg tgacgctggt gtcgctgatg
    27001 gaccactcgc cgggccagcg ccagttcgcc aatcgcgaga agtatcgcga atattatcag
    27061 ggcaaatact ccctcactga tgcgcagatg cagcagtacg aagaagagca actggcgctc
    27121 gccgcacgct ggtcgcagcc gaatcgcgaa tccatcgccg ccctgtgccg cgcgcgaaaa
    27181 attgcgcttg ccagccacga tgacgccacc cacgcccacg ttgctgaatc tcaccagctt
    27241 ggcagcgtga tcgccgaatt tcccaccacg ttcgaagcgg cggaagcctc gcgcaagcat
    27301 ggcatgaacg tgctgatggg cgcgccgaat attgtgcgcg gcggctcgca ctccggcaac
    27361 gtggcggcca gtgaactggc gcagcttggc ctgctggata tcctctcttc cgactactac
    27421 cccgccagcc tgctcgatgc ggcatttcgc gtcgccgatg acgagagcaa ccgctttacg
    27481 ctgccgcagg cggtgaagct ggtgactaaa aatccagcgc aggcgcttaa tctccaggat
    27541 cgcggggtga ttggcgaggg caaacgcgcg gacttggtgc tggcgcatcg caagggcaat
    27601 cacattcata tcgaccacgt ctggcgtcag ggtaaaaggg tgttctgatg atgggaaaac
    27661 tgatttggtt aatggggccg tccggctccg ggaaagatag cctgctggcg gaactccgcc
    27721 tgcgggaaca aactcagcta ctggtggcgc atcgctacat cacgcgcgac gccagcgccg
    27781 gaagcgaaaa ccatatcgct ctgagcgagc aggagttttt tacccgcgcg gggcagaacc
    27841 tactggcctt aagctggcac gctaacggtc tgtattatgg cgtcggcgtc gagatcgacc
    27901 tctggctgca cgccggattc gacgtgctgg tcaacggctc acgcgcccat ctgccgcagg
    27961 cgcgggcgcg ctatcaatcg gcgctgctgc ccgtctgttt acaggtttcg ccggagatcc
    28021 tccgccagcg cctggaaaac cgtggccgtg aaaacgccag tgaaattaac gcccgcctgg
    28081 cgcgcgccgc ccgctatact ccacaggatt gccatacgct caacaatgac ggcagcctgc
    28141 gccagtcggt cgacacgctg ctgacgctga ttcatcagaa ggagaaacac catgcctgct
    28201 tgtgagcttc gcccggccac gcagtatgat accgacgcgg tttacgcgct gatttgtgag
    28261 ctaaaacagg cggagtttga ccaccacgca tttcgcgtgg gctttagcgc caatctgcgc
    28321 gacccgaaca tgcgctatca tctggcgctg ctggatggcg aggttgtcgg catgatcggc
    28381 ctgcatttgc agtttcatct gcatcatgtc aactggatcg gcgaaattca ggaactggta
    28441 gtgatgccgc aggcgcgcgg cctgaacgtc ggtagcaagt tgctggcgtg ggcagaagaa
    28501 gaagcccgcc aggccggggc cgaaatgacc gagctttcga ccaacgtgaa gcgccacgac
    28561 gcgcaccgtt tctatctgcg agaaggctac gagcagagtc acttccgctt caccaaggcg
    28621 ctgtaacatg agtctgaccc tcacgctcac cggcaccggc gg
//
LOCUS       DAJVRF010000041        27665 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJVRF010000041 DAJVRF010000000
VERSION     DAJVRF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27665
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.041"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(317..523)
                     /locus_tag="OQN13_001625"
     CDS             complement(317..523)
                     /locus_tag="OQN13_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCS1615008.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            836..925
                     /gene="kdpF"
                     /locus_tag="OQN13_001626"
     CDS             836..925
                     /gene="kdpF"
                     /locus_tag="OQN13_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCS1615009.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            925..2598
                     /gene="kdpA"
                     /locus_tag="OQN13_001627"
     CDS             925..2598
                     /gene="kdpA"
                     /locus_tag="OQN13_001627"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCS1615010.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2621..4669
                     /gene="kdpB"
                     /locus_tag="OQN13_001628"
     CDS             2621..4669
                     /gene="kdpB"
                     /locus_tag="OQN13_001628"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCS1615011.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4678..5250
                     /gene="kdpC"
                     /locus_tag="OQN13_001629"
     CDS             4678..5250
                     /gene="kdpC"
                     /locus_tag="OQN13_001629"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCS1615012.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5243..7927
                     /gene="kdpD"
                     /locus_tag="OQN13_001630"
     CDS             5243..7927
                     /gene="kdpD"
                     /locus_tag="OQN13_001630"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCS1615013.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7924..8601
                     /gene="kdpE"
                     /locus_tag="OQN13_001631"
     CDS             7924..8601
                     /gene="kdpE"
                     /locus_tag="OQN13_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCS1615014.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8876..8980
                     /locus_tag="OQN13_001632"
     CDS             8876..8980
                     /locus_tag="OQN13_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCS1615015.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9291..11489
                     /gene="speF"
                     /locus_tag="OQN13_001633"
     CDS             9291..11489
                     /gene="speF"
                     /locus_tag="OQN13_001633"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCS1615016.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11486..12805
                     /gene="potE"
                     /locus_tag="OQN13_001634"
     CDS             11486..12805
                     /gene="potE"
                     /locus_tag="OQN13_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCS1615017.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12861..14501)
                     /gene="pgm"
                     /locus_tag="OQN13_001635"
     CDS             complement(12861..14501)
                     /gene="pgm"
                     /locus_tag="OQN13_001635"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCS1615018.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14527..15072)
                     /gene="seqA"
                     /locus_tag="OQN13_001636"
     CDS             complement(14527..15072)
                     /gene="seqA"
                     /locus_tag="OQN13_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCS1615019.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15257..16021
                     /gene="ybfF"
                     /locus_tag="OQN13_001637"
     CDS             15257..16021
                     /gene="ybfF"
                     /locus_tag="OQN13_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1615020.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16161..16454
                     /gene="ybfE"
                     /locus_tag="OQN13_001638"
     CDS             16161..16454
                     /gene="ybfE"
                     /locus_tag="OQN13_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCS1615021.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16594..17124
                     /gene="fldA"
                     /locus_tag="OQN13_001639"
     CDS             16594..17124
                     /gene="fldA"
                     /locus_tag="OQN13_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCS1615022.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17334..17420
                     /gene="uof"
                     /locus_tag="OQN13_001640"
     CDS             17334..17420
                     /gene="uof"
                     /locus_tag="OQN13_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCS1615023.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17413..17859
                     /gene="fur"
                     /locus_tag="OQN13_001641"
     CDS             17413..17859
                     /gene="fur"
                     /locus_tag="OQN13_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCS1615024.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17944..18270)
                     /locus_tag="OQN13_001642"
     CDS             complement(17944..18270)
                     /locus_tag="OQN13_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1615025.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQYLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18320..19726)
                     /gene="chiP"
                     /locus_tag="OQN13_001643"
                     /pseudo
     CDS             complement(18320..19726)
                     /gene="chiP"
                     /locus_tag="OQN13_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
     gene            complement(20304..21968)
                     /gene="glnS"
                     /locus_tag="OQN13_001644"
     CDS             complement(20304..21968)
                     /gene="glnS"
                     /locus_tag="OQN13_001644"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCS1615026.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22228..24174)
                     /gene="nagE"
                     /locus_tag="OQN13_001645"
     CDS             complement(22228..24174)
                     /gene="nagE"
                     /locus_tag="OQN13_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCS1615027.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            24507..25307
                     /gene="nagB"
                     /locus_tag="OQN13_001646"
     CDS             24507..25307
                     /gene="nagB"
                     /locus_tag="OQN13_001646"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCS1615028.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25367..26515
                     /gene="nagA"
                     /locus_tag="OQN13_001647"
     CDS             25367..26515
                     /gene="nagA"
                     /locus_tag="OQN13_001647"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCS1615029.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            26524..>27665
                     /gene="nagC"
                     /locus_tag="OQN13_001648"
                     /gene_synonym="nagR"
     CDS             26524..>27665
                     /gene="nagC"
                     /locus_tag="OQN13_001648"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCS1615030.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHR"
BASE COUNT         6499 a         7050 c         7246 g         6870 t
ORIGIN      
        1 cccggctgaa ccctggacaa tgctaccgcc atactgcgtc atgtcgccct gacgcgccgc
       61 cggttttccg ctcatttctc atccttgttt tatcgggtta tttgtccgct gacaggcaag
      121 accctacaga tttataagaa agccttaagt gagatatccg tcagatagaa acaattagga
      181 aaatatttat atatatttca tgaatatatg tttcgattgg tataacggtg tctaaatatt
      241 cctctgaaca tcttcctgat acaaaaaacc gccagcgttt ccgctggcgg ttttcaatgt
      301 attcagaact cagcaatcag taaaaatcac cggttgcctt ttcggcctga tccatccaca
      361 ccggtttatc gctggttttc gaccagacgc ggtgcaggta gctgtaaaag cgggcgcggt
      421 ctttccagaa aagcatgaaa ggcagcgcca gaacgcccgc tgcaaccgca tatgtacggc
      481 gtaaaaagat aagccatgca ggatattctc tgtagagttc catacttttc tccccctttt
      541 gtatctaccc ggtgaatagc accggaaaaa tgaatttgtt tatctgatga aaatagtacc
      601 gccttttgtg taattttact actcatccga ccacttattt ttgcttattg atggtttatt
      661 tacattcact ctgtaattaa gttacacaaa agttaaatta atactaaaca ttagttaaat
      721 catggctttt gccattttta tacttttttt acaccccgcc cgcagatttt tgcgaaatct
      781 ttgcagccag aattctactc ttccggtatc acttttaggc cactggaggt gcactgtgag
      841 tgcaggcgtg atagccggcg tgttgctggt gtttttatta ctgggttatt tggtttatgc
      901 cctgatcaat gcggaggcgt tctgatggct gcgcaagggt tcttactgat tgccacgttt
      961 ttactggtat taatggtgct ggcgcgtcct ttaggcagcg ggctggcgcg gctgattaat
     1021 gacattcctc ttccaggtac agcgggcgtt gaacgtatac tctttcgttt acctggcgtc
     1081 tctgaccatg agatgaactg gaagcaatat ctttgtgcca ttctcggcct gaacatgctg
     1141 gggctggcgg tgctgttttt tatgttgctc ggtcagcact atctgccgct taatccacag
     1201 cagttgccgg ggctgtcgtg ggatctggcg ctgaataccg ccgtcagctt tgtcacgaat
     1261 accaactggc aatcttatag cggtgaaacc acgttgagct atttcagcca gatggcaggc
     1321 ttaacggtac aaaactttct ttctgccgcc agcgggattg cggtgatttt tgccttcatc
     1381 cgtgcgttta ctcgccagag catgagcaca ctcgggaatg cctgggtcga tctgctacgc
     1441 atcacgttat gggtgctagt ccctgtggcg ttgttgatcg cactgttttt tattcaacaa
     1501 ggtgcgctgc aaaactttct gccctatcag gcagtgaata ccgttgaagg agcgcaacag
     1561 ctgttaccca tgggaccagt agcttctcag gaagcgatca agatgctcgg tactaacggc
     1621 ggtggcttct ttaatgccaa ctcgtcgcat ccgtttgaaa acccaaccgc actgaccaac
     1681 ttcgtgcaga tgctggcgat cttcttgatc ccaacagcgc tgtgctttgc ctttggtgaa
     1741 gtgacgggcg atcgccgcca ggggcgcatg ttgctgtggg cgatgtcagt gatttttgtc
     1801 atctgcgtag gcgtggttat gtgggcagaa gttcagggta atcctcatct gctggcgctc
     1861 ggcgcagaca gcagcatcaa tatggaaggt aaagagagcc gtttcggcgt gctggtcagt
     1921 agcctgtttg cggtcgtgac tacggcggcg tcctgtggcg cggtgattgc gatgcatgat
     1981 tcgtttaccg ctctcggcgg catggtgccg atgtggctaa tgcaaattgg tgaagtggtg
     2041 ttcggcggtg tcggttctgg tctttacggc atgatgctgt ttgtcctgct ggcggtgttt
     2101 attgccgggc tgatgattgg tcgtacaccg gaatatctgg gtaaaaaaat cgacgtacgc
     2161 gagatgaaac tgactgcact ggcgattctg gttactccaa cactggtact gatgggcgcg
     2221 gcgttggcga tgatgaccga cgccggacgt agcgccatgc ttaaccctgg cccgcatggt
     2281 tttagcgaag tgctgtacgc cgtgtcgtcc gccgctaaca acaacggcag cgcctttgcc
     2341 ggattaagcg ccaactctcc gttctggaac tgtttactgg cgttctgcat gtttgtcggt
     2401 cgcttcgggg tgattatccc ggtgatggca attgccggtt cgctggtgag taaaaagagc
     2461 caacccgcca gctccggcac gctgccaacg cacggcccgc tgtttgttgg cctgttaatc
     2521 ggcaccgtgt tgctggttgg cgcactgacc tttatccctg ccctggcgct tggtccggtg
     2581 gcggaatatc tctcctgatg atattgagcg agcactgaat atgagtcgta aacaactggc
     2641 gctattcgaa ccaacacttg tcgttcaggc gctgaaagaa gcggtgaaaa aattaaaccc
     2701 gcaggcgcaa tggcgcaatc cggtgatgtt tatcgtctgg atcggcagtc tgctgaccac
     2761 ctgtattagc atcgcgatgg caagcggtgc gatgcctggc aatgcgctgt ttagcgcggc
     2821 aattagcggc tggctgtggg tcaccgtact gttcgctaat ttcgccgagg cgctggcaga
     2881 aggccgcagt aaagcgcagg ccaacagtct taaaggggtg aaaaaaactg cctttgcccg
     2941 caagctgcgt gagccgaaat atggcgctgc ggcggacaaa gttcctgccg atcaactgcg
     3001 taaaggtgac atcgtgctgg tagaagctgg cgatattatc ccctgcgatg gtgaagtcat
     3061 tgaaggcggc gcatcggtcg atgaaagcgc catcaccggg gaatcggcac cggtgatccg
     3121 tgaatccggc ggcgattttg cctccgtcac cggcggcacg cgtattcttt ctgactggct
     3181 ggtgattgag tgtagcgtta accccggtga gacatttctg gatcggatga tcgcgatggt
     3241 ggaaggcgca cagcgacgca aaacaccgaa cgagattgcc ctgactatcc tgctgattgc
     3301 tctgaccatc gtctttttac tggcaaccgc cacgctgtgg ccgttttccg cgtggggcgg
     3361 taatgccgtg agtgtaacgg tactggtggc gctgctggtc tgtctgatcc caaccactat
     3421 tggcggcctg ttgtcagcga tcggcgtcgc cgggatgagt cggatgctgg gcgcgaatgt
     3481 gattgccacc agcggacgtg cagttgaagc ggcaggtgac gttgacgttc tgctactgga
     3541 taaaaccggc accatcacac tcggtaaccg tcaggcgtcg gagtttatcc ccgcgcaggg
     3601 cgtggatgaa aaaacgctgg ctgacgccgc acaactggct tcgctggctg atgaaacgcc
     3661 ggaaggtcgc agtattgtga tcctcgccaa gcaacgtttt aacctgcgcg agcgcgatgt
     3721 gcagtcgctc catgccacct ttgtaccgtt tactgcgcaa agccggatga gcgggatcaa
     3781 catcgacaac cgcatgatcc gtaaaggttc tgtcgatgcc attcgccgcc atattgaggc
     3841 taacggcggt cacttccctg ccgatgttga tcaaaaagtc gatcaggttg cgcgtcaggg
     3901 agccacgccg ctggtggtgg tggaaggttc tcgtgtgctg ggcgttattg cgctgaaaga
     3961 tatcgtcaaa ggcggtatta aagagcgctt cgcccagctg cgcaaaatgg gcattaaaac
     4021 ggtgatgatt accggcgata accgtctgac tgccgccgcg atagctgcgg aagcgggtgt
     4081 cgatgatttt ctcgccgaag caacaccgga ggccaagctg gcattgatcc gtcagtatca
     4141 ggcggaaggt cgtttggtag cgatgaccgg cgacggcacc aacgatgctc cggcgctggc
     4201 gcaggcagat gtcgcggtgg cgatgaactc cggcacccag gcggcgaaag aggcgggcaa
     4261 tatggtggat ctcgactcta acccgaccaa gttgatcgag gtggtgcaca ttggcaaaca
     4321 gatgctgatg acccgtggct cgctgaccac cttcagcatt gccaacgatg tggcgaaata
     4381 cttcgccatt attccggcgg cgttcgcggc aacatatccg cagttaaatg cgctgaacat
     4441 catgcgcctg cattcgcccg actccgccat tctcagtgcg gtcattttca acgccttgat
     4501 tatcgtcttt ttgattcctc tggcgttaaa aggcgtgagt tataaaccgc ttaccgcttc
     4561 tgccatgttg cgccgtaact tatggattta cggtctgggt gggctgctgg tgccgtttat
     4621 tggtatcaaa gtcattgatt tactgctgac cgtttgcggt ctggtgtgag gtttaccatg
     4681 agtggattac gtccggcatt atcgacattt ctctttctgt tattgattac tggcggcgtt
     4741 tacccgctgc tgaccaccgc actggggcaa tggtggtttc cctggcaggc caacggttcg
     4801 ttgatccgtg aaggtgatac ggtgcgtggc tcggcattaa tcgggcagaa ttttaccggc
     4861 aacggctatt ttcatggtcg cccgtcagca acggctgaaa tgccctataa tccacaggct
     4921 tctggcggga gcaatctggc ggtcagtaac cctgagctgg ataaactaat agccgcacgc
     4981 gttgctgcat tacgggccgc taacccggat gccagcgcga gcattccggt tgaactggtg
     5041 acggcatcgg caagcgggct ggacaataat atcaccccgc aagcggcggc ctggcagatc
     5101 ccacgcatcg cgaaagcgcg taatctcagc gttgaacagc tcacgcagct gatcgcaaaa
     5161 tacagccaac aaccgctggt gaaatatatc ggccagccgg tcgtcaacat tgtcgaactc
     5221 aatctggcgc tggataagct tgatgaataa cgaaccctta cgtcccgacc ccgatcgtct
     5281 gctggaacaa actgccgcgc cgcatcgggg gaagctgaaa gttttcttcg gtgcctgtgc
     5341 aggcgtcggg aagacctggg cgatgctggc agaagcccag cgactgcggg cgcaagggct
     5401 ggatattgtg gttggcgtgg tagaaaccca cgggcgaaaa gataccgccg caatgctgga
     5461 agggctggct gttctgccgc caaaacgcct ggcgtaccgt gggcggcaca tctgcgagtt
     5521 tgacctcgat gccgccctcg cccgccgtcc ggcgctgatc ttaatggacg aactggcgca
     5581 cagtaatgcg ccaggttctc gtcaccctaa acgctggcag gacatcgaag agctgctgga
     5641 agcaggcatt gatgttttca ctaccgtcaa cgttcagcat ctggaaagtc tgaatgatgt
     5701 ggtcagcggc gtcaccggaa ttcaggtacg ggaaaccgtg cccgatcctt ttttcgatgc
     5761 cgccgacgac gtggttctgg tggacttgcc cccggacgat ctgcgccagc ggctgaaaga
     5821 aggcaaagtc tatattgccg ggcaggcgga gcgcgccatt gaacattttt tccgcaaagg
     5881 caatctgatc gccctgcgcg aactggcact gcgccgtact gccgatcgcg ttgatgagca
     5941 aatgcgcgcc tggcgggggc atcctagcga agagaaagtc tggcacacgc gcgacgcgat
     6001 ccttttatgc atcggccata acaccggtag tgagaagctg gtccgcgcgg cggcgcggct
     6061 ggcgtcacgg ctgggtagcg tctggcacgc ggtgtatgtt gaaacccctg ccctgcatcg
     6121 cttaccggag aaaaaacgtc gtgcaattct cagcgcctta cgtctggcgc aggaactggg
     6181 cgcggagacg gcaacacttt ctgatccagc ggaagagaaa gcggtagtgc gttatgcccg
     6241 tgaacataat ctcggcaaga ttattctcgg tcgcccggcc tcgcgccgct ggtggcgtcg
     6301 ggaaacgttt gctgaccgac tggcgcgcat cgcccccgat ctcgatcagg tgctggtcgc
     6361 gcttgatgaa ccacccgccc gcacgattaa caacgcgccg gatagccgct cttttaaaga
     6421 caagtggcgg gtacagattc agggatgcgt ggttgccgcc gcgttatgcg ccgttatcac
     6481 cttaattgcc atgcagtggc tgatggcgtt tgatgccgcc aacctggtga tgctgtatct
     6541 gcttggcgtg gtggtggtgg cgctatttta tggacgctgg ccttcggtgg ttgccaccgt
     6601 cattaatgtg gtgagtttcg atctcttttt tatcgcccca cgcggcacgc tcgccgtctc
     6661 tgatgtgcaa tatctgctga ccttcgcggt gatgttaacc gtcgggctgg tgatcgggaa
     6721 ccttactgct ggcgtgcgtt atcaggcgcg ggtagcccgt taccgcgagc aacgcacacg
     6781 gcacttatat gaaatgtcga aagctctggc ggtgggccgc agtccgcagg acatcgccgc
     6841 caccagcgaa caatttattg cctccacgtt tcatgcccgc agtcaggtgt tgttgcccga
     6901 tgacaacggt aaattgcagc cgttaacaca tccgcaagga atgacgccat gggacgatgc
     6961 catcgcgcag tggagttttg ataaaggcct gcctgcgggc gcgggcaccg acacgttacc
     7021 cggtgtaccg taccagattt tgccgctaaa aagcggcgag aaaacctacg ggctggtggt
     7081 ggtggagccg gggaatctgc gccagttgat gatcccggaa cagcagcgcc tgctggagac
     7141 gtttacgctg ttagtcgcca atgcccttga gcggctgacg ctaaccgcca gcgaagaaca
     7201 ggcgcggatg gcaagcgaac gtgaacagat ccgcaacgcc ctgctggcag cgctttcgca
     7261 tgatttacgc acgccgctta cggtgctgtt tggtcaggca gaaatcttaa cgctcgatct
     7321 ggcaagcgaa ggatcacccc acgcccgcca ggccagcgag attcgtcagc atgtgctgaa
     7381 cactacccga ctggtgaata atctactgga tatggcgcga attcagtccg gcggctttaa
     7441 tttgaagaaa gagtggttaa cgctggaaga agtagtcggc agcgcgctgc aaatgctgga
     7501 accgggttta tcgtcgccca tcaatctttc tctgccagaa ccgctgacct taatccacgt
     7561 tgacgggcca ctctttgaac gggtgctgat taatctgctg gagaacgcgg tgaaatatgc
     7621 gggtgcgcag gccgaaattg gtatcgatgc ccactttgag ggcgaaaatc tacaactgga
     7681 tgtctgggat aacggccccg gtcttccgcc aggccaggag cagacgatat ttgataagtt
     7741 tgctcgcggg aataaagagt cggcggtgcc gggtgtcggg cttgggttgg caatttgtcg
     7801 ggcgattgtg gatgtacacg gcggcaccat taccgcgttc aaccgaccgg aaggtggtgc
     7861 ctgttttcgt gttacacttc cccagcaaac cgctcctgaa cttgaagact ttcatgagga
     7921 tatgtgacaa acgttctgat tgttgaagat gaacaggcta ttcgtcgctt tctgcgcacg
     7981 gcgctggagg gcgacgggat gcgcgtcttt gaggccgaaa ctctgcaacg cggcttgctg
     8041 gaagcggcaa cccgtaagcc agatttgatt attctcgatc tcggcctgcc cgatggtgat
     8101 gggattgagt ttatccgcga cctgcgccag tggagcccgg tgccggtgat tgtgctttcc
     8161 gcacgcagcg aagagagcga caagatcgcc gcgctggatg ccggagcgga tgattatctg
     8221 agtaagccgt ttggcattgg cgaattgcag gcccgtctgc gcgtcgcttt acgccgccac
     8281 tccgccaccg ccgcgcccga tccgctggtg aaattttccg atgttaccgt cgatttagcc
     8341 gcccgcgtga ttcaccgggg tgaggaagag gtgcatctca caccaattga gttccgcctg
     8401 ctggcggtgc tgctcaacaa tgccggaaaa gtactcaccc agcgccagct ccttaaccag
     8461 gtgtgggggc caaacgcggt cgaacacagt cactatttgc gtatttatat gggacatctg
     8521 cgacaaaaac tggaacagga tccggcccgg cctcgccact tcattactga aaccggtatt
     8581 ggatatcggt ttatgctttg aattgttatt caataaattc gcttaaatag tgattaattt
     8641 attattaaac ctgcgccgaa tattattatt tggcaggttt aatttcttaa cgcaaatata
     8701 aaacacgaaa ttacaacatt taagtaacaa aacagcatct aatatggcga tattcataat
     8761 taatgattta ttatctgggg tatgatcgtt ttttgttgat cttcttcaca gattatagcc
     8821 atttcatgga tagaataact ctaccttcaa ctgacacagc aagaggtaaa ggtaaatgga
     8881 aaataataac cgcttaatgc ctcatataag gcggacaacc catatcatga agtttgccca
     8941 tcgtaatagt tttgactttc atttctttaa tgcccgctag tcttctgact aaagggcacc
     9001 ccaacgtaca ggtctccctg actttaagca ttacaggtta atacctgtat tcctcggtgc
     9061 tcatatactg ctaacccttt taaactctaa ataattcgag tcgcagcact tgcaacttga
     9121 ggtatgacga gtatagccag ttaccgggct ggtctgggtt attgcatctg caaaaagcaa
     9181 actactgatt tatttatcag cggtggagct ttgctttttt tccggcgtga tcgatttctc
     9241 ctttgagaaa ttgaggacct gctattacct gaaataaaga gatgaataaa atgtcagaat
     9301 taaaaattgc cgttagccgt tcttgcccgg attgtttttc cactcatcgt gcatgcgtga
     9361 atattgacga aagtaattat attgacgttg ccgccattat tttatcagtc aatgatgttg
     9421 aacgtggaaa actcgatgaa atagacgcta ctggctatgg cattcctgtt tttattgcaa
     9481 cggaaaatga agaacgtgta ccagcagaat atctgccacg tatttctggt gtcttcgagc
     9541 attgcgaatc acggaaagag ttttatggac gtcagttaga aaccgctgct agccattatg
     9601 aaacgcaact gcgcccgccc ttcttccgcg cactggtcga ttatgtcaat cagggcaata
     9661 gcgcttttga ctgcccgggt catcagggag gtgagttttt ccgtcgtcat ccggcaggga
     9721 atcagttcgt ggagtacttc ggagaaacgc tgttccgttc cgacttatgc aacgcagacg
     9781 tagcaatggg cgatctgctg attcacgaag gcgcgccatg cattgcacag caacatgcgg
     9841 caaaagtgtt taatgccgat aaaacatact tcgttctaaa tggcacttca tcttctaaca
     9901 aagtggtttt aaacgccctg ctaacaccgg gtgatctggt gctgtttgat cgcaataacc
     9961 acaaatctaa ccaccacgga gcgttgctac aggctggtgc aacaccggtt tatctggaaa
    10021 cggcacgtaa cccgtatggc tttatcggtg gcattgatgc gcactgtttt gaagaaagtt
    10081 acctgcgtga gctgatcgcg gaagtcgcac cgcagcgggc aaaagaggct cgtcctttcc
    10141 gcctcgctgt gattcagtta ggcacctacg acggtacgat ttataacgcc cgccaggtgg
    10201 tggataaaat tggtcatctg tgtgactaca ttctgtttga ctccgcatgg gttggctatg
    10261 agcaatttat cccgatgatg gctgactgtt cgccgctgtt gctggatctt aatgagaacg
    10321 atccgggtat tctggttacg caatccgtac ataaacagca ggctggtttt tctcaaactt
    10381 cacaaatcca taaaaaagac agccacatca aagggcaaca gcgttatgta ccgcacaaac
    10441 gcatgaacaa cgcctttatg atgcacgcct ccaccagccc gttctatccg ctgtttgccg
    10501 cactggatat caacgccaaa atgcatgaag gtgtcagcgg tcgtaatatg tggatggatt
    10561 gtgtggtaaa tggcattaat gcccgcaaac tgatcctcga taactgtcag catattcgtc
    10621 cgttcgtacc tgaactggtg gatggtaaac cctggcagtc gtatgaaaca gcgcaaattg
    10681 cggttgatct gcgcttcttc cagtttgtac caggggaaca ctggcattct tttgaaggct
    10741 atgcagagaa tcaatacttt gtcgatcctt gcaaactgtt gctgacaacc ccaggtattg
    10801 atgcacgtaa cggcgaatat gaagcgttcg gtgtacccgc gacgattctt gctaacttcc
    10861 tgcgcgaaaa tggcgtagtg ccggaaaaat gcgatcttaa ctccatcctc ttcctgctga
    10921 ctccggcaga agatatggcc aaacttcagc aacttgttgc cctgctggta cgcttcgaaa
    10981 aactgcttga gtccgacgcg ccattagcag aagtgctgcc ttccatctac aaacagcatg
    11041 aagagcgcta cgccggttat accctgcgtc agttgtgcca ggaaatgcat gatttgtatg
    11101 cccgccacaa cgtgaaacaa ctgcaaaaag agatgttccg caaagcgcac ttcccacacg
    11161 tcagcatgaa tccgcaagaa gccaactacg cctatttacg cggtgaagtg gagctggttc
    11221 gtctgccgga tgccgaaggt cgtatcgctg ccgaaggggc gcttccttat cctccgggtg
    11281 tgctgtgtgt tgttccgggt gaaatctggg gtggtgctgt tttgcgttac ttcagcgctc
    11341 tggaagaagg gatcaacctg ctgccaggtt ttgcaccgga gctgcagggt gtctatatcg
    11401 aagaacatga tggtcgtaag caagtttggt gctatgtcat caagcctcgt gatgcgcaaa
    11461 gcaccctgtt gaaaggggaa aaattatgag tcaggctaaa tcgaacaaaa tgggcgtcgt
    11521 tcagttaacc atactgacga tggtcaacat gatgggctcc ggtatcatca tgctgccgac
    11581 aaagcttgcc gaagttggga caatttcaat tatctcctgg ctggtgacag ccgtaggctc
    11641 aatggcactg gcatgggcat tcgcaaaatg cggtatgttc agccgtaaat caggtggtat
    11701 gggcggttat gccgagtatg ctttcggtaa atccggtaac tttatggcga actataccta
    11761 cggcgtctca ctgctgattg ctaacgtcgc gattgctatt tcggcggttg gttacggcac
    11821 cgaactgctc ggcgcgagtt tgtcgccagt gcagattggt cttgcgacca tcggggtgct
    11881 gtggatttgt accgtggcta actttggtgg tgcgcgcatt accgggcaaa tcagtagcat
    11941 taccgtgtgg ggggtcatta ttccggtggt aggcctgtgc attatcggct ggttctggtt
    12001 tagcccgacg ctttacgttg attcctggaa tccgcatcat gcaccgttct tcagtgcagt
    12061 aggttcttcc atcgctatga cgctgtgggc ttttctcggt ctggagtctg cgtgtgcgaa
    12121 tactgatgta gtggaaaacc cggaacgtaa tgtgccaatc gcggtactcg gcggtacgtt
    12181 aggtgcggcg gtgatttata tcgtctccac caacgtgatt gcagggattg tgcctaatat
    12241 ggagctggca aattcaacgg caccatttgg cctggccttc gcacagatgt tcacaccaga
    12301 agtgggtaaa gtcattatgg cgctgatggt gatgtcctgc tgcggttcgc tgcttggctg
    12361 gcagttcacc attgcccagg tgtttaagtc ttcatctgat gaaggctact tccctaaaat
    12421 tttctcccgt gtaaccaaag tggatgcacc ggtgcaggga atgttgacca ttgtgattat
    12481 ccagagcggg ttggcgctga tgacgattag cccgtcgcta aacagtcagt tcaacgtgct
    12541 ggttaacctg gccgtggtga ccaatatcat tccgtatatt ctgtcaatgg cggcattagt
    12601 cattattcag aaggtcgcta atgtgccccc ctcaaaagcg aaagttgcaa actttgttgc
    12661 ttttgttggc gcgatgtata gcttttatgc gctgtactca tccggggaag aagccatgct
    12721 gtacggttcc atcgtgacct tccttggttg gacactgtat ggtctggtat caccacgctt
    12781 tgaactgaaa aataaacacg gttaaaaaag caaagtaaaa aagggcgatc ttgcgaccgc
    12841 ccttttttta ttaaatgtgt ttacgcgttt ttcagaactt cgctaacaat ctcaaccgct
    12901 tctttctcaa tctgcttgcg atgttcttca ccgaggaagc tttcgcagta gatcttatat
    12961 gcgtcttccg tgcctgacgg acgcgcggcg aaccagccgt tgtcagtcat cactttcaga
    13021 ccgccgatag aagcaccatt gcccggtgcc gccgtcaggc gcgcggtgat cgggtcgcct
    13081 gccagggtgc tggcgctcac catttccgga gacagcttag acagcgccgc tttttgtgcg
    13141 gaagtcgcag ctgcctgcaa acggttgtag ctcggcgcac caaagcgttt tgccagttcg
    13201 ttgtagtgtt cctgcgggtt cttaccggtg acagcggtga tttccgccgc cagcagacac
    13261 atgatgatgc cgtctttgtc ggtggaccac ggcgtgccgt cgaaacgcag gaaggaagcc
    13321 cctgcgctct cttcgccgcc aaagccgaag ctgccgtcga acagaccatc aacaaaccat
    13381 ttgaaaccta ccgggacttc taccagcttg cggcccaagt cattgaccac acggtcgatc
    13441 atcgcagaag aaaccagcgt tttaccgaca gcaacatctt tgccccactg cggacgatgc
    13501 tggaacaggt agttgatcgc caccgccagg tagtggttcg gattcatcaa acctgccgga
    13561 gtgacgatac cgtgacggtc ataatccggg tcgttagcaa acgccagatc gaacttatca
    13621 cgcaatgcca gcaggcccgc catcgcacac tcggaggagc agtccatacg gatcgcgccg
    13681 tctttatcga ggtgcataaa gcggaaggtt tgatcgacct gatcgttaac gatggtcagg
    13741 ttgaggttgt aatactcgcc aattcgcttc cagtattcga taccggaacc acccagcgga
    13801 tcaacgccca gcgtcaggcc cgttttctga atcgccgcca tatcaacgat atcggccaga
    13861 ccttccacaa actgctgcac cagatcctgc tctttcacat gaccggatgc catcgcttcg
    13921 tcgagggaga tacgcttcac gcctttcagg ccatcggcca tcagtgcgtt ggcacgatct
    13981 tccaccactt tagtgacgtt ggtatcagcc gggccaccat ttggcggatt gtacttgata
    14041 ccaccatctt ccggcgggtt atgggagggt gtaatcacga taccgtctgc cagcgggcca
    14101 ccttttttat tatgaaccag gatggcattg gaaacggcag gcgtcggagt gaagccattg
    14161 ttttcctgca caatgacatc aacgccgttc gctgccagca cttccagtac ggaaatgaac
    14221 gcaggctcgg acagggcgtg agtatcttta cccacatagc aagggccagt gatgccgttt
    14281 ttcgcacgtt cttcagcaat tgcctgagcg atcgccagaa tgtgcggctc gttaaagctg
    14341 tggcgcgctg cactgccacg gtgaccggaa gtaccgaatt tcaccgcgtg ctccgcattc
    14401 cctgcttctg gtttcagtac ataatattgc gccgtcagtt gggcgacgtt aatcaaatca
    14461 ctctgttgtg caggttggcc tgcacgattg tggattgcca ttgctttgtc ctttgtctgc
    14521 aacgttttag atagttccgc aaaccttctc aatcaattcc gccgggaatt gcatcgactg
    14581 catgatgtgt tcgatcatgc tgcatttacg gccggtgttg gtgttggtga tcacccaata
    14641 cggcgtgcct ggcacatgtt tcggcttggt ctgattacca tttttcagca gcgtttgttc
    14701 atctgccgca aagtaaacgc gtgtacgacc gtgcaacgat tccgttgctt cggcaaacgc
    14761 ctgggcgtca agagaatata gtgtagacaa cagcagcata aagcgattga ccgctcgctt
    14821 ttgctctgcg tattcgtcag aaagcagaag ttcacgcatt gcgcgaacct tgtctttaat
    14881 cgttttgacc ggcttcgctt cgacgatagc aggtgacgca acgcgaacct ctttcgtcac
    14941 cggagcagca ggctgtgatg cggcggaaaa tttcaacata cgccgtaaaa tgtcggatgc
    15001 gctctcgccg atatgcttag tgtggctggc aatatagctg tagagttcat catcaacttc
    15061 aatcgttttc atcttaatcc agtacagtgt cttagctgaa tacaagtcgc caagagtata
    15121 tgaataaatc ccggcagcgg atagcgtcaa gcctggcttg cagaaaaact cggaataaac
    15181 cttatttgtt gcagccctgt ggcacaatgg tcaacataat accctaaccc gacaacgcga
    15241 aaaaaagaac tttgccatga aattgaatat ccgcgcgcaa actgcacaaa accagcacaa
    15301 taattctccc atcgttcttg tccatggtct gtttggtagc ctcgacaacc ttggcgtact
    15361 ggctcgcgat ctggtaaacg atcacaatat catccaggtt gatatgcgta accacggtct
    15421 ttcaccgaga gatccggtaa tgaattaccc ggcgatggcg caggatcttg ttgatactct
    15481 ggatgcacag cagatcgaca aagcaacatt tatcggtcac tccatgggcg gtaaagcggt
    15541 aatggcactt acggcactgg cccgcgatcg catcgataaa ctggtggcga tcgatatcgc
    15601 accggtcgac tatcacgtac gccgtcatga tgagattttc gcggctatca acgcggtcag
    15661 tgaatcggac gcacaaactc gccagcaagc agcagcaata atgcaccagc atcttaatga
    15721 agaaggggtg attcagtttc tgttgaaatc ttttgttgac ggggagtggc gctttaacgt
    15781 gccggtattg tgggatcagt atccgcatat tgtgggttgg gagaaaatcc cggcatggga
    15841 tcaccctgcc ctgtttatcc ctggcggcaa ttctccgtat gttagcgagc agtaccgtga
    15901 tgatttactg gcacaatttc cacaggcacg ggcgcatgtg attgcaggcg cgggtcactg
    15961 ggtccatgct gaaaaaccgg atgcggtatt acgcgctatc cgtcgctatc tcaatgatta
    16021 acgcaactga ttaaaaaccc agcgtccgcg tcgctttccg cgcgtgggcg ttggcgtgac
    16081 tcatcgcgct gatgtattat ggcgcgctat ccatccgggc agaggcctgg ctgattgttt
    16141 cccccgaagt caccaagatc atggccaaag aacaaacgga ccgtacgaca ttagatctgt
    16201 tcgcgcacga gcgtcgaccg ggacgaccga aaactaatcc gctttcgcgc gatgaacagc
    16261 tgcgtattaa taaacgcaac cagctaaaac gcgacaaagt acgtggcctt aagcgtgtcg
    16321 aactgaagct gaacgcggaa gctgtcgagg cgctgaacga gctggcggag tcgcgcaata
    16381 tgagccgtag cgaactgatc gaagagatgt taatgcagca actggcggcg ctgcgtagtc
    16441 agggcatcgt ttaaatttcc actttcatgt agcacagtgt gcagtcctgc tcgtttgctg
    16501 attccgcaag actgcctgtt ctgctatgat tgcctttatc cgtgggcaat tttccacccc
    16561 catttcaata agtttcaaga ggttatttca ctcatggcta tcactggcat ctttttcggc
    16621 agcgacaccg gtaataccga aaatatcgca aaaatgattc aaaaacagct tggtaaagac
    16681 gttgccgatg tccatgacat tgcaaaaagc agcaaagaag atctggaagc ttatgacatt
    16741 ctgctgctgg gcatcccaac ctggtattat ggcgaagcgc agtgtgactg ggatgacttc
    16801 ttcccgactc tcgaagagat tgatttcaac ggcaaactgg ttgcgctgtt tggttgtggt
    16861 gaccaggaag attacgccga atatttctgc gacgcattgg gcaccatccg cgacatcatt
    16921 gaaccgcgcg gtgcaacgat cgttggtcac tggccaactg cgggctatca tttcgaagca
    16981 tcaaaaggtc tggctgatga cgaccacttt gtcggtctgg ctatcgacga agaccgtcag
    17041 ccggaactga ccgctgaacg tgtagaaaaa tgggttaaac agatttctga agagttgcat
    17101 ctcgacgaaa ttctcaatgc ctgatgtgat gcggcgtaga ctcatgtcta cgccgtatta
    17161 atagataatg ccaatcaaaa taattgctac aaatttgtaa cttttgctgt tgtacctgta
    17221 caatgtcccg gtgttcaagt ggccttgccg ttgtaaatgt aagctgtgcc acgtttttat
    17281 taacaatatt tgccagggac ttgtggtttt catttaggcg tggcaattct ataatgatac
    17341 gcattatctc aagagcaaat tctgtcactt cttctaatga agtgaaccgc ttagtaacag
    17401 gacagattcc gcatgactga taacaatacc gccctaaaga aagctggcct gaaagtaacg
    17461 cttcctcgtt taaaaatcct agaagttctt caggagccgg acaaccatca cgtcagtgcg
    17521 gaagatttat acaaacgtct gatcgatatg ggtgaagaaa ttggtctggc tacggtatat
    17581 cgcgtactga accagtttga cgacgctggt atcgtcaccc gccacaattt tgaaggcggt
    17641 aaatccgtat ttgaactgac acagcaacat caccacgatc acctgatctg cctcgactgc
    17701 ggcaaggtta tcgaatttag tgatgattcc atcgaagcgc gtcagcgtga aattgccgca
    17761 aaacatggca ttcgcctgac taaccacagt ctctatcttt acggtcactg tgccgaaggc
    17821 gattgccgcg aagatgaaca tgcgcacgaa ggcaaataag ccagcctgaa tgagaaaaag
    17881 ccaacctgcg ggttggcttt tttatgcaag ggaaataaga aaagcgagag ttacagctct
    17941 cacttatttg ttattactgc gaatttcctg ccaaacttta tcacaatcgg ctttcaccgc
    18001 ctgatcattt ccggtttgcg ttgcacgcag atactgctga taatccagta cgcggacact
    18061 ttcctggtcg gcaaaatgct gatgttgttt ctctttcttc agcacgttta acacgctctg
    18121 gcaggcttca attttttctg gcgaaccttg tgcggtgttg atacaggcgc tgtatgcctc
    18181 tttcagacga ctgtcttcct gtggcgcggt tgattgcgca caagctacca gccccgatgc
    18241 cattatggcg atgagaatca gttttttcat agcaaattcc tcaaacgtgc ggcaggcaca
    18301 aaacctgccg cgtcgggcat cagaagatgg tgaatggtgc gattaccata aatttcacgt
    18361 cacgctcatc ctggaagatg ttgccgtaac caccgcccca gcttgggatg tcggagtggt
    18421 tgtcgtattc ggtgaagtgc agtttgaaca tcgtgccttt ggcgcgaccg tcctgaatgg
    18481 tgtagaccgc atccaggctg tatgcagact cttcaatagt ccggtttttg tcgtagtacg
    18541 catccggatt gctctgctag gtcgcaggtt tagcatccca tgcgtaaacg taggaagcgc
    18601 cgatggcgaa gcctggaaga ttccagtttt tcaggtcata catcgcaccg aagaagaccg
    18661 ctttttcgcc gttagcgttg aagtcggaac ggttatccca ccagatatcc aggcggccgt
    18721 tggaggaagc gtaggttgga gtcatacgtt gcaggaagta tccctgctga ccgtcagcct
    18781 taacccaggt gccttcgagg cgcaaatcca ctacgtcagc cgcccggtaa ccaaaggtca
    18841 acgcttgcag ccaggcggtg ccgtcataaa ggtcgttgac gctgcgatcg tcaactttat
    18901 cgcgcgtacc atagaactgg tagctggtgg ttaacgggct accggcgata tcaaatttgt
    18961 agctggcttt ggcaaaatat tgatcgatat acccttccgc ctgaccaaat gccgcttcca
    19021 gtacgaagtt atttttgaag tcgtatttcg ccccaaggga gtgcagataa tcaactttgg
    19081 tggttttatc gttctgataa aactcatcca tttccagatg ccacggcgct ttgtattcgt
    19141 tggtccacat gtaggagaaa ctcaacgcac cagcatcgcc gtaatcaaaa ttcgccccgg
    19201 cttccgcccc ctgataagta cctggcataa agctccagtg cggcgctaac agcgtttgac
    19261 cagttggctg aatgtaacct gccctcgccc aaaccggacc gtatttaaat ttggccgcag
    19321 ctttatacag gcttataccg cttttgtcgc cggaccagtc ttcgtcatag gctttattac
    19381 tttttgaaaa cgcgatttcg ttcgggtggg agctgtcgcc gttttccgcc atttcaatcg
    19441 ccgtaaacgc agcaatatca aggccgaaca tatcagcagc ataaccggac tgaaaatcga
    19501 ggttggcatt ccaggtggag tgagaaaggt tggttttgta tttgtcgccg tcggtaacat
    19561 ctttacggtc gcgttcacgc tgccagtaat atataccgcc ggttaaggtt gaatcgtcga
    19621 tgaatccttc ggcgcgagcc tccggcattg ccataaagcc cgacattgct gtaacaccgg
    19681 cgatagccag cgccagcgta ctacgtttgc cactaaacgt acgcatgggt taatcctctt
    19741 tgacgtataa attgctgcac caaaggtgaa cagcgaaaaa ataaaaatta aaaaagtagc
    19801 gttgagaaaa gtctcgctga ctacaggaac agactatttt tcgcgacgcg aattatcaat
    19861 ctctttctgt aaccagaatg taagaacatg agcaaccgca catattttga tgatttttgt
    19921 tacaaggctt aagtttgcgg ttgaaaggct tgcagaagaa taaaagcgtg aaattccagc
    19981 aagatgaaag cggttacatt ttatttaatt atgttttgtt attttaaatt tattttatgg
    20041 ataaattgcg aagcggaatt aattcgcgaa gaaaagcaga aaaaacgccg ctgaacgcgg
    20101 cgttttggat tgtttgcata tttcttgccg gatgcggcgt gaacgcctta accggcctac
    20161 aaaagcacgc aaactcaata tattgcagag atcatgtagg cctgataagc gtagtgcatc
    20221 aggcaattta gcgtttgaaa ctgtttgtct gttccttgcc ggatgcgacg taaacgcccc
    20281 atccggcata gcgaaactta aaattactcg cctactttcg cccaggtatc acgcagccca
    20341 acggtgcggt taaataccgg tttttccgcc gtagaatggc ggctatcgag gcagaagtaa
    20401 ccttcacgct caaactggaa tgctttaccc gcaaccgcat ctttcagcga cggttcagca
    20461 aagccctgtt tgatcaccag cgattccggg ttaatcaccg acaggaaatc atccgcagca
    20521 ccagggttcg gcacgctgaa cagacggtcg tacagacgga tttcaaccgg cagcgcatgt
    20581 gccgcgctca cccagtgaat aacacctttg actttacgac catctgccgg atctttgctt
    20641 aaggtgtcgg cgtcataagt acagaagatg gtggtgatat tgccttcggc atctttctcc
    20701 acgcgttctg ccttaatgac gtaagcatta cgcagacgca cttctttacc cagcaccaga
    20761 cgtttgtact gcttgttagc ttcttcgcgg aaatcggcgc gatcgatcca aatctcaccg
    20821 ctaaacggca cctggcggct acccatttcc ggtttgttcg gatggttcgg catggtaacc
    20881 atttcgcctt cgccctgata gttttcgata accagtttca ccggatcgat aaccgccatt
    20941 gcgcgcgggg cgttttcgtt gagatcttca cggatgcagg attccagcga cgccatctca
    21001 atggtgttgt cctgcttggt cacgccgatg cgtttgcaga actcacgaat agaagcagca
    21061 gtgtaaccac gacgacgcag accggaaatg gtcggcatac gcgggtcatc ccagccttca
    21121 acgtgcttgt cggtcaccag caggttcagc ttacgcttgg acatcacagt gtattccaga
    21181 ttcaggcgcg agaattcgta ctggcgcggg tgaacaggaa tggtgatgtt gtccagcacc
    21241 cagtcgtaca ggcgacggtt gtcctggaac tcaagcgtac acagagagtg cgtaatacct
    21301 tccagggcat cgctgatgca gtgggtgaag tcgtacatcg ggtagatgca ccacttgttg
    21361 ccagtctggt ggtgttcagc aaatttaatg cggtacagca ccggatcgcg catcacgata
    21421 aacggtgaag ccatgtcgat tttcgcacgc aggcaggctt taccttcttc aaaaccaccg
    21481 gcacgcattt tttcgaacag cgccaggttc tcttcaacgc tgcggtcgcg gtacgggctg
    21541 tttttacccg gttgcgtcag ggtgccgcgg tattcgcgga tctgttccgg cgtcagttca
    21601 tcaacgtacg ccaggccttt attgatcagt tcgatcgcat aggcgtggag ctgatcaaaa
    21661 taatcggagg agtaacggac gttaccagac cagtgaaaac ctaaccactc tacgtcgttt
    21721 ttgatcgact caacatactc gatatcttct tttaccgggt tagtgtcgtc gaaacgcagg
    21781 ttgcactggc ctttatagtc ctgggcgatc ccgaagttca ggcagataga tttcgcatgg
    21841 ccaatatgca gatagccatt cggctccggc gggaaacggg tgtgtactgt ggtgtgctta
    21901 ccactggcca gatcttcatc gatgatctga cggataaagt tagtcgggcg ggcttctgcc
    21961 tcactcatcg tggattcctc aaagcgtaaa caacgtataa cggcgtatga tcttataagc
    22021 gggacaggct gacaactgtt agttagcgaa aatcgaaaat ctcactgaga ttatggggat
    22081 gattgatgaa aaaaaaaagc ggcagagaag atctccaccg cttttgccag tcccgcgtca
    22141 ttgttggatg cgcgtcttat ccaaccagga ctgagctttt gtaggcctga taagacgcgt
    22201 caagcgtcgc atcaggcata aagcagatta ctttttgatt tcatacagcg gtgtttgacc
    22261 cgccacaaca ttgccctgag ctttaatgat caagccactg aaatcgtcga tattgctgca
    22321 aaccaccggg ctaatcatcg aacgggcgtt agcgttcagg taatccagat ccatttccag
    22381 aatcggttgc cctgcgctca cctgcgcgcc ctcttccacc agacgtttaa agcctttacc
    22441 ttccagcgct acggtgtcga tacccatgtg gacgacaatc tccgcgcctt tttcggtttc
    22501 taggcagaac gcgtggttgg tgttgaagat tttcacgatt gtccctgcgg ctggtgatac
    22561 gacgatttta tctgtcggtt tcaccgccac accgtcaccc accgctttgc tggcgaatgc
    22621 ttcgtcagaa acctgatcca gtgccacgac atcaccggta atcggcgata ccagctccgc
    22681 gatagatacc gcgtttggta cagcctgcgg ttttgctaca ggagcggcag ttgccggagt
    22741 tgcttcagct gacgcagctg ctaccgggcc acgggcaacg actttcttca tcgcatcgcc
    22801 aatggattct gctttcgcgc caacaatcac ctgaatggtc tgtttgttca gttttactac
    22861 accagaagca cccagacgtt tacacatcgc atcgttgacg cgagctgagt cagccacggt
    22921 aaggcgcaga cgggtaatac aggcgtcaat cgctttcagg ttgtcagtac cgccaaccgc
    22981 agcaatatag ttggttgcca gttgagtcag accttcttca gtattgctgt tagcctcttc
    23041 agttacgatc tcgtcttctt tatcttcacg acccggcgtt ttcaggttga acatgcggat
    23101 aaccaaactg aacaccacga agtagatagc gaagaagaca acacccatca ccagcagcat
    23161 ccagacgttc tggctggcgg ccggcaggtt atacatcaac gcgtagtcga tagcccccgc
    23221 agagaaagag aagccagcat ggatacccag cagcgttgcc acaaacaggc tgataccggt
    23281 cagcagtgcg tgcaggaggt acagcagcgg agcaaggaac atgaacagga attccagcgg
    23341 ctcagtcaca ccggtcagga acgcagtaac agcaacagaa agcagcatcc cgccaaccat
    23401 cggacgacgc tctttcggtg ctgcgaagta catcgccagc gccgcacccg gcagaccgaa
    23461 catcatgatc gggaagaagc cggacatgaa catccccgcg gtgccgtcac cagcgtagaa
    23521 acggttgatg tcgccgtgga aaaccgtacc cgccgcgttg gtgaattcac caatctggaa
    23581 ccaggcgatg gtgttcagca cctgatgcag accggttggg atcagcagac ggttgatgaa
    23641 accaaagata ccggaaccca gcgcgcctgc agaaacgatc cactcgccgc ctgcatggat
    23701 agcgtgctgt actggcggcc agacgtaacc aaaaatggcc gccagcacca ggcagaaaaa
    23761 gccggtggca atcggcacaa agcgtttgcc gccgaagaag ctcaggaagt ccggcagttt
    23821 aatatcggac caacggttat aggctgcgcc accaaccaga ccggtaatga tacccgccag
    23881 tacacccatg ttaatttctg ggttgatggt caccatcgct ttggttaaca caaagtaacc
    23941 taccgcaccc gccagcgccg ccgcacctgc gctgtctttc gaccagctgg atgccacacc
    24001 gatggcgaag attaatgcga ggttatcaaa aatcgcaccg cccgcctggg caataaacgc
    24061 aacgttaagt aaatctggct gaccgaatcg cagcaacagt gccgccaccg gcagcaccgc
    24121 gatagggagc tgtaacgccc taccgagtcg ctggaaaaaa cctaaaatat tcatcttatt
    24181 ccccctacga gaaccctatt tggctcgttt aaagccgtat ttttattttg ctgcaaattg
    24241 tactgccgat gttctgtaat cagattgtta gatcatctgc tacagagtgt gtgaaaattt
    24301 aattcgtatc gcaaattaaa cgcgtgcctt ttgtgagttt tgtcaccaaa tatcgttatc
    24361 atcactccct tttactggct aaagcagaaa acttatttta tcattcaaaa aatcaggtcg
    24421 gattgacgcc tgtctgcgca aatccaggtt acgcttaaag atgcctaatc cgccaacggc
    24481 ttacatttta cttattgagg tgaataatga gactgatccc cctaactacc gctgaacagg
    24541 tcggcaaatg ggctgctcgc catatcgtca atcgtatcaa tgcgttcaaa ccgactgccg
    24601 atcgtccgtt tgttctgggc ctgccgactg gcggcacgcc gatgaccacc tataaagcgt
    24661 tagtcgaaat gcataaagca ggccaggtca gctttaagca cgttgtcacc ttcaacatgg
    24721 acgaatatgt cggtctgccg aaagagcatc cggaaagcta ctacagcttt atgcaccgta
    24781 atttcttcga tcacgttgat attccagcag aaaacatcaa ccttctcaac ggcaacgccc
    24841 cggatatcga cgccgaatgc cgccagtatg aagaaaaaat ccgttcttac ggaaaaattc
    24901 atctgtttat gggcggtgta ggtaacgacg gtcatattgc atttaacgaa ccggcgtctt
    24961 ctctggcttc tcgtactcgt atcaaaaccc tgactcatga cactcgcgtc gcgaactctc
    25021 gtttctttga taacgatgtc aatcaggtgc caaaatatgc cctgactgtc ggtgttggta
    25081 cattgctgga tgccgaagaa gtgatgattc tggtgctggg tagccagaaa gcactggcac
    25141 tgcaggctgc agttgaaggt tgcgtgaacc acatgtggac catcagctgt ctgcaactgc
    25201 atccaaaagc gatcatggtg tgcgatgaac cttccaccat ggagctgaaa gttaagactt
    25261 taagatattt caatgaatta gaagcagaaa atatcaaagg tctgtaattg ttatccctgc
    25321 cctggctcct tgctcagggc aatatttttt aaaatcgggg ggcagaatgt atgcattaac
    25381 ccagggccgg atctttaccg gccacgaatt tcttgatgac cacgcggttg ttatcgctga
    25441 tggcctgatt aaaagcgtct gtccggtagc ggaactgccg ccagagatcg aacaacgttc
    25501 actgaacggg gccattctct cccccggttt tatcgatgtg cagttaaacg gctgcggcgg
    25561 cgtacagttt aacgacaccg ctgaagcggt cagcgtggaa acgctggaaa tcatgcagaa
    25621 agccaatgag aaatcaggct gtactaacta tctgccgacg cttatcacca ccagcgatga
    25681 gctgatgaaa cagggcgtgc gcgttatgcg cgagtacctg gcaagacatc cgaatcaggc
    25741 gttaggtctg catctggaag gtccgtggct gaatctggta aaaaaaggca cccataatcc
    25801 gaattttgtg cgtaagcctg atgccgcgct ggtcgatttc ctgtgtgaaa acgccgacgt
    25861 cattaccaaa gtgaccctgg caccggaaat ggttcctgcg gaagtcatca gcaaactggc
    25921 aaatgccggg attgtggttt ctgccggtca ctccaacgcg acgttgaaag aagcgaaagc
    25981 cggtttccgc gcggggatta cctttgccac ccatctgtac aacgcgatgc cgtatattac
    26041 cggtcgtgaa ccgggcctgg cgggcgcgat cctcgacgaa gctgacattt attgcggtat
    26101 tatcgctgat ggcctgcatg ttgattacgc caacattcgt aacgctaaac gccttaaagg
    26161 cgacaaactg tgtctggtta ccgacgccac cgcgccagca ggtgccaaca ttgaacagtt
    26221 catttttgcg ggtaaaacaa tatactaccg taacggactt tgtgtggatg agaacggtac
    26281 gttaagcggt tcatccttaa ccatgattga aggcgtgcgt aatctggtcg aacattgcgg
    26341 tatcgcactg gatgaagtgc tgcgtatggc gacgctctat ccggcgcgtg cgattggcgt
    26401 tgagaaacgt ctcggcacac tcgccgcagg taaagtagcc aacctgacct cattcacacc
    26461 tgattttaaa atcaccaaga ccatcgttaa cggtaacgag gtcgtaactc aataagagaa
    26521 agtatgacac caggcggaca agctcagata ggtaatgttg atctcgtaaa acagcttaac
    26581 agcgcggcgg tttatcgcct gattgaccag tacgggccaa tctcgcggat tcagattgcc
    26641 gagcaaagcc agcttgcccc cgccagcgta accaaaatta cgcgtcagct tatcgaacgc
    26701 gggctgatca aagaagttga tcagcaggcc tccaccgggg gccgccgcgc tatctccatc
    26761 gtcaccgaaa cccgcaattt ccacgcaatc ggcgtacggc ttggtcgtca cgacgccacc
    26821 atcaccctgt ttgatctcag cagcaaagtg ctggcagaag aacattaccc gctgccggaa
    26881 cgtacccagc aaacgctgga acatgccctg ttgaatgcca ttgctcagtt tattgatagc
    26941 taccagcgca aactgcgcga gctgatcgcg atttcggtga tcctgccagg gcttgttgac
    27001 ccggacagcg gcaaaattca ttacatgccg catattcagg tagaaaactg ggggctggta
    27061 gaagctctgg aagaacgttt taaagtgacc tgtttcgttg gtcacgatat ccgtagtctg
    27121 gcgctggcgg agcactactt cggtgcaagt caggattgcg aagactccat tctggtgcgt
    27181 gtccatcgcg gaaccggggc cgggattatc tctaacgggc gcatttttat tggccgcaac
    27241 ggtaacgtcg gtgaaattgg ccatattcag gtcgaaccgc tgggtgaacg ctgccactgc
    27301 ggcaactttg gctgcctgga aactatcgcc gccaacgctg ccattgaaca acgggtgttg
    27361 aatctgttaa agcagggcta ccagagccgc gtgccgctgg acgactgcac catcaaaact
    27421 atctgcaaag ccgcgaacaa aggcgatagt ctggcgtcgg aagtaattga gtatgtcggt
    27481 cgtcatctgg gtaaaaccat cgccattgct atcaacttat ttaatccgca aaaaattgtt
    27541 attgccggtg aaatcaccga agccgataaa gtgctgctcc ctgctattga aagctgcatt
    27601 aatacccagg cgctgaaggc gtttcgtact aatctgccgg tggtacgttc tgagctggat
    27661 caccg
//
LOCUS       DAJVRF010000042        27536 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJVRF010000042 DAJVRF010000000
VERSION     DAJVRF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27536
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.042"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(198..1521)
                     /locus_tag="OQN13_001649"
                     /pseudo
     CDS             complement(198..1521)
                     /locus_tag="OQN13_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1521..3137)
                     /locus_tag="OQN13_001650"
     CDS             complement(1521..3137)
                     /locus_tag="OQN13_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCS1615031.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3433..4149
                     /locus_tag="OQN13_001651"
     CDS             3433..4149
                     /locus_tag="OQN13_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1615032.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4146..5807)
                     /locus_tag="OQN13_001652"
     CDS             complement(4146..5807)
                     /locus_tag="OQN13_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCS1615033.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6003..6431)
                     /gene="ibpB"
                     /locus_tag="OQN13_001653"
     CDS             complement(6003..6431)
                     /gene="ibpB"
                     /locus_tag="OQN13_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCS1615034.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6543..6956)
                     /gene="ibpA"
                     /locus_tag="OQN13_001654"
     CDS             complement(6543..6956)
                     /gene="ibpA"
                     /locus_tag="OQN13_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCS1615035.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(6950..7022)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7262..7594
                     /locus_tag="OQN13_001655"
     CDS             7262..7594
                     /locus_tag="OQN13_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCS1615036.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7596..8810)
                     /locus_tag="OQN13_001656"
     CDS             complement(7596..8810)
                     /locus_tag="OQN13_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCS1615037.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            8911..9975
                     /gene="cbrA"
                     /locus_tag="OQN13_001657"
     CDS             8911..9975
                     /gene="cbrA"
                     /locus_tag="OQN13_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCS1615038.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(9972..11309)
                     /locus_tag="OQN13_001658"
     CDS             complement(9972..11309)
                     /locus_tag="OQN13_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1615039.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11384..12532)
                     /gene="dgoD"
                     /locus_tag="OQN13_001659"
                     /pseudo
     CDS             complement(11384..12532)
                     /gene="dgoD"
                     /locus_tag="OQN13_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(12529..13146)
                     /gene="dgoA"
                     /locus_tag="OQN13_001660"
     CDS             complement(12529..13146)
                     /gene="dgoA"
                     /locus_tag="OQN13_001660"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCS1615040.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13130..14008)
                     /gene="dgoK"
                     /locus_tag="OQN13_001661"
     CDS             complement(13130..14008)
                     /gene="dgoK"
                     /locus_tag="OQN13_001661"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCS1615041.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPAHFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14005..14694)
                     /gene="dgoR"
                     /locus_tag="OQN13_001662"
     CDS             complement(14005..14694)
                     /gene="dgoR"
                     /locus_tag="OQN13_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCS1615042.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            14663..15295
                     /locus_tag="OQN13_001663"
                     /pseudo
     CDS             14663..15295
                     /locus_tag="OQN13_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15341..16153)
                     /gene="yidA"
                     /locus_tag="OQN13_001664"
     CDS             complement(15341..16153)
                     /gene="yidA"
                     /locus_tag="OQN13_001664"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCS1615043.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16268..16666)
                     /locus_tag="OQN13_001665"
     CDS             complement(16268..16666)
                     /locus_tag="OQN13_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCS1615044.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(16906..19320)
                     /gene="gyrB"
                     /locus_tag="OQN13_001666"
     CDS             complement(16906..19320)
                     /gene="gyrB"
                     /locus_tag="OQN13_001666"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCS1615045.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19349..20422)
                     /gene="recF"
                     /locus_tag="OQN13_001667"
     CDS             complement(19349..20422)
                     /gene="recF"
                     /locus_tag="OQN13_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCS1615046.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20422..21522)
                     /gene="dnaN"
                     /locus_tag="OQN13_001668"
     CDS             complement(20422..21522)
                     /gene="dnaN"
                     /locus_tag="OQN13_001668"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCS1615047.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(21527..22930)
                     /gene="dnaA"
                     /locus_tag="OQN13_001669"
     CDS             complement(21527..22930)
                     /gene="dnaA"
                     /locus_tag="OQN13_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCS1615048.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23227..23307
                     /locus_tag="OQN13_001670"
     CDS             23227..23307
                     /locus_tag="OQN13_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1615049.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            23537..23677
                     /gene="rpmH"
                     /locus_tag="OQN13_001671"
     CDS             23537..23677
                     /gene="rpmH"
                     /locus_tag="OQN13_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCS1615050.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            23694..24053
                     /gene="rnpA"
                     /locus_tag="OQN13_001672"
     CDS             23694..24053
                     /gene="rnpA"
                     /locus_tag="OQN13_001672"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCS1615051.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24017..24274
                     /gene="yidD"
                     /locus_tag="OQN13_001673"
     CDS             24017..24274
                     /gene="yidD"
                     /locus_tag="OQN13_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCS1615052.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24277..25923
                     /gene="yidC"
                     /locus_tag="OQN13_001674"
     CDS             24277..25923
                     /gene="yidC"
                     /locus_tag="OQN13_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCS1615053.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26029..27393
                     /gene="mnmE"
                     /locus_tag="OQN13_001675"
     CDS             26029..27393
                     /gene="mnmE"
                     /locus_tag="OQN13_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCS1615054.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6614 a         7468 c         7033 g         6421 t
ORIGIN      
        1 tagctgcgga ggtgcgccaa tatccgtcat ccgccaatac attgaacaac agcaaacacc
       61 tggttaggtg gaaaaccgcg ccttatatcc ccgccctgaa ggacggggtt ttacggcgca
      121 ccggataaat ttggttatgc gccgtcagcc gtgctaaaga atacggacca gcacccaacg
      181 gatgccagtc cacacaatta acgtaactct ggccagtacg ctttgttggc ctccagcaac
      241 tcatccagaa tagctttggc gaccgaggcg ctcggtaccg ttttcgataa cgtaatcgcc
      301 tgccacaggt gctgatatga acgctgttcc caggcgtcca cgaccagttt ttccaccgcc
      361 acttgctgac tcattaaccc tttctgaaac tgcgggatat cgccgaccac cagtggttct
      421 ggtccgttgt gcccgaccag acacgggatc tcgaccatcg cttcatcatc aaagttatgg
      481 attgccccgt tgttaggcac aatcagcaac atccgctcct gagtgttgaa ggcaattgcc
      541 gccgccagat cgacgatgta tgacgcatgt tcgtcaatct ccagcttggc cgccgctgaa
      601 tttcctgccg ctgtaatggc gcggcaagca tcgaaaacct gtttctcgcg atgttccatt
      661 cacctcattc gcgcgggtat gttcagggtt ggaatgctga acaacataat ccgggaagag
      721 ataatatttc agataggtgt tcggcaatgt atccgggtcc agtgcctgga catcccgcgc
      781 ttttgcatag gtgtcgttcc agctcgcttc agtatgtgta tcttgctgcg gtggaacata
      841 accgtgcttc gagacatatt gccgcagctg gggcatcagg tcgttgccct gcaaatcttc
      901 aattgatgtc caccagccaa agtggttcag gccgtagtag cgcacgcgca tctgtttgcg
      961 atcttgcagc ccaacaattt gcgccatccg gctttcaata ccgattggca tgtcacagat
     1021 gttgaggatt ttcgcattcg ggcgcagacg acgcgtggct tctgcgacaa tggctgccgg
     1081 gttggagtag ttgagcatcc aggcatttgg tgaatatttt tccatataat ccaccagttc
     1141 caggacgccg ccaatggaac gcatgccgta cgctattccg ccgggtccgc aagtttcctg
     1201 accaacaaca ccgtggcgca gtgggatttt ttcatccagt tcgcgcatcg ggtacttgcc
     1261 gacgcggatg tgcgccatga caaaatcaac gtcgctgaac gccacttcag gatcggtggt
     1321 gtaactaaac gcaatgtccg gcgctttctc tttaaggatg actttacagg cttcggcaat
     1381 cacttcctgc cgcgcgccat cgttatcata aaatttcagt gcacgaagcg ggaaacgctc
     1441 ctgattcgcc aggagcatca acacgatgcc tggcgtaaag gtgcttccac cgcctgcgac
     1501 aaccactgag aatttggtca ttatactgcc tccgtaatgg caacattttc tgctgattga
     1561 tgagaattaa ttaagctatc gagctgttca cgcagctggg atacatgcag accaatgatc
     1621 acctgaatgg catcgccact acggaagacg ccgtgcgctc ccagcttttt aaagacttcg
     1681 tcatccagcg tttgtgacat gtcatgcagt gcaatgcgta gacgcgtcgc acaattgtta
     1741 atgctggaga tattgccgac cccgcccagg gcttgcagga taccggcagc ctgatccagc
     1801 tctttttttg gctctgccgc ggtggtttgg cctcgcgagg ctttgtattc ggcttttgag
     1861 tagagtttca cttccgcatc ttcacgtccc ggcgtgcaca tgttgaactg cagaatcagg
     1921 gtgcggaaaa ccacgaagta cagcagggta aagcacaacc caatggcgat ttgggtcagc
     1981 atcatatccg cgtggttgct gaacatcggg atccagtttt gcggtaaaac ctggtcaatc
     2041 agacctccgc ccatgttgcc caccacacca aagagataca tcacggtcga cattgaggcc
     2101 gcaagcacag cgtgtaccgc aaacagcaac ggtgaaatga acaggaaggt aaattccagc
     2161 ggttcggtaa ttcccaccag catggcggtt aaggttgcgg gaatcagcaa gcccgccact
     2221 tttacccgat tttccggtgc ggcagtgaag tacatcgcta aagaaatgcc cacggcacca
     2281 aagatttttg agttaccgtg cagggcaaaa ccgccttccg ggaacaacga tttcagcggc
     2341 tcggcgctca aactgaactc ttgcagatgc tgcgcccagt acatctgaat gccgccttca
     2401 acagctgccg gacccaagat aaacggtccg tagatgaagt ggtgtaaacc ggttgggatc
     2461 agaatacgtt cgaggaaggt gtaaacccag accccaagcg cacccgccga acgcaggaac
     2521 gcttgcagag attcaatccc cgtttgtact tttggccagc cgagcaatgt cagccaggca
     2581 caggggatca tcaccaggaa ggcgataatc accacataag acgtcccctg gaaaatgccg
     2641 agaaaaaccg gcagtttttt atcgaacaga cggttatgca gcgccgtcac aatgccggaa
     2701 atgataattg cgccgataat gctggtatcg agggttttaa tcccggccat cattgtcaga
     2761 ccgctacctg ccaccgcgtc ctgagtgaaa tcgacgccga agtagcttcc ccaggtcatt
     2821 cccatcgcgt tgatgaaata gttccaggtc aggaaactca ccatcaccgc cagacaagca
     2881 cgtccctgcg cttgcttagc aaggccaatg ggtaaaccga cagcaaaaat cagcggcata
     2941 ttacggaata ccgtccaacc gccctcttca ataatgtgta cgatttgcgc gaataaactg
     3001 ttcggatcgg tcagtgattc cccgacaaac atcgggtttt gcagcaagat ggcaagaccc
     3061 accacaatcc cggcgaaggg aaacagcagc actggcgtaa acatcgcgcc gccaaagcgt
     3121 tgaatttgac tgagcatttc cacatcctct tttctcaatt ctgtaagtag gaagcctgat
     3181 tggttatgcg gcaggcctgg agcaaggata ggaagatgcg atcggcagaa catgacgacc
     3241 caatgggatt cgtgatcgct ttcatgcttt tcgttgggcg gtagagatct acgttttcgc
     3301 gaaaaagtag acatctgtga gcacaggtac acagcatcga acacatcttt aaaaaaaaga
     3361 tgttttttca atcgattaag cagaacttgt gtgcgcatta cccgggcttg caggcaaaaa
     3421 agagatctag agatgatcta caaaagcatt gcggagcggt taagaattcg acttaactcc
     3481 gcagatttca cgctaaacag ccttcttccg ggtgaaaaaa agctggcgga agagtttgcg
     3541 gtatcgcgga tgaccatccg taaagccatt gacctgctgg tagcgtgggg gctggtggtc
     3601 cgccgccacg gtagtggcac ttacctggtg cgcaaagatg tactgcatca aaccgccagt
     3661 ctgaccgggc tggtggaggt gttaaaacgg cagggaaaaa ctgtcaccag ccaggtgctg
     3721 atttttgaaa tcatgcctgc gcctccggcc attgccagcc agttacggat tcaaatcaac
     3781 gagcagatct acttctcccg tcgcgttcgt tttgtggaag ggaaaccgct gatgctggaa
     3841 gacagctata tgccggtaaa actgttccgt aatctttcgc tgcaacatct ggaagggtcg
     3901 aagtttgaat atattgaaca agagtgcggg attttgattg gcggtaatta tgaaagcctg
     3961 acgccggtgc tcgccgatag actgctggcg cggcaaatga aggtagcgga acacacgcca
     4021 ctgctgcgga tcacctcgtt gtcatatagc gagagcgggg agtttttgaa ttattcagtg
     4081 atgttcagaa atgccagcga ataccaggtg gagtaccatt tacggcgact ccacccggaa
     4141 aagagttaac cgatactcca gaagagcacc gccagtaatt ggggggtgat aatgcgcagg
     4201 aacatcacta acggatagac agtggcgtaa gagagcgccg ccgcaccgct agttggatga
     4261 agattattag caaacgccag cgccggagga tcggtcatgg aacctgccag catcccgcac
     4321 atggtcaggt aattcatttt ggctaacatc cgcgccagaa tgccaacagt aatcagcgga
     4381 acggcggtga tcagggcacc ataaccaatc cagcttagcc cttcgccatt gaccagggta
     4441 ttcacaaaat ccccaccaga tttcagacca acgaccgaga ggaacagcac gatccccagc
     4501 tcccgcagcg cgaggttggc acttggcggc ataaaccagt acagcttgcc gatactgccg
     4561 atacgcccga ggatcaacgc cataatcagc agaccgcccg ccagccccag tttcaacgcg
     4621 gccgggaatc ccggcacaaa gacgggaata gaacctaaca atacgccaag cccgatgcca
     4681 ataaacactg gcagcatctg aacctgttgc agtttttgct gcgcattccc cagcacattg
     4741 gcaacggcat caattgcgga cggacgcccc accaggttga ggatatcgcc gaactgcagg
     4801 ctgatatcgc cactggcgac cagttcgacc ccggcacggt tcaggcgcga gataacaacg
     4861 tcatagcgtt ctttaaagtg caggtcgcga atacgttttc cgagcacgtt ttcattggtg
     4921 accaccacac gctcgacgcg caaatcagtg tctttcgtgg acagcgaagt atcgacctcc
     4981 tgaccaatca ccagttgcgc attatgtaaa tccgctggct gacccaccag atgcagcaaa
     5041 tcgcccagtt ggataatggt atctggcgaa ggaactttta gggtttcttc gcgtttcaga
     5101 cgcgagcaga taattttgtc gccgttgaga atcggtacat ctttaatggc taaatcatgc
     5161 aggttagggt tctcaacgcg aatattgata gtcttgatca gcgcgccgcc attggtgcgt
     5221 gaagactcgt gctgctgagc ttctgtctcg acattgacgc ggaaaataac ccgcaacatc
     5281 cacatggtga acaaaatccc gcaaatgccg aatggatacg ccatcgcgta actcatcccc
     5341 atctgatcga ccatttccat tggtgtaccc aggtcgcgca aaatctgctg ccctgccccc
     5401 agcgctggcg tattggtaac cgcaccggag aaaatcccca gcactaccgg cagtggaata
     5461 tcaaacagtt tatgcaggat ggcggtaacc agaccaccga tgatgacgat cagaacagca
     5521 aacaggttga ggcgtaatcc ggagacgcgc aatgaggcaa agaagcccgg ccctacctga
     5581 atcccgatgg tataaacgaa caggatcagg ccaaattcct gaataacatg cagcatatcg
     5641 ctacttaatg tcatccccgc ctgagaaaca aaatggccga cgatgatccc accaaacagc
     5701 acgccgccaa tacctaatcc tatgccgcga aatttgacgt tgccgataaa caaaccgacg
     5761 actgccacca aagccagaat actgaccgtt aatgctatat cactcatcgt ccatttctct
     5821 tgagataaca ttttaattgc tacggattat ggctgaatcg gtgaagcaga tttgccttat
     5881 agcgcacatt attacgggaa agaacgtgcc gaaatatctt aaacagtcga aatttaacgc
     5941 attgttgaag aagaaagaca aaaaaagccc caccagaatg gcggggcaaa gagaatagct
     6001 agttagctat ttaacgcggg acgttcgctg atagcgatac gctgcgctgc gatgggttca
     6061 ggctcattac gaattaaatc aatatgcagt aaaccgttta cgaaagttgc gccagagact
     6121 tccatatttt cagccagcgt aaagctcagg ctaaatggct gattcataag cccttgatgc
     6181 agccattttt tctcttcttt tggctgctcc ggcgtgcctt ttacgctcag gcgcgtacct
     6241 tccagttgaa tctctaaatc ttcctgacgg aaacctgcca gcgcaagggt aatgcggtag
     6301 tggttatcgt cgcttttctc aatgttgtac ggcgggaagc tctgactttc accggcgttt
     6361 tgcagtgcgt tggccagttt gtcaaaaccg atccattgac gcatcagtgg ggataaatcg
     6421 aagttacgca tagtcatttc tccttctaag aagcgagtaa gtacctgcaa atccgaagat
     6481 tcgcatatgc tccctgacgg cgagcatgga gatgtcaggc cgcgccaggc ggccttaagg
     6541 aattagttga tttcgatacg gcgcggtttt ttcgcttccg gaatcacgcg ttcgagatcg
     6601 atatacagca aaccatttac caggttagcg ccacgaacat gaatgttctc agctaactgg
     6661 aatttgcgtt caaagttgcg ttcagcgatg ccctggtaca gataggtgcg ctctttttgt
     6721 tcgtcggcgt gagcaccttt caccaccagc agattatcct gggcggtaat ttccagttcg
     6781 ctctcagcaa aaccagccac agcgatagca atgcggtaat ggttttcgtc taccagttca
     6841 acgttatacg gagggtagcc gccattactc tggctctggt tgttttctaa gtggttaaac
     6901 aaacggtcaa atccaatagc agaacggtaa agcggggata aatcaaagtt acgcataatc
     6961 aatagctcct gaaatcagcg agaatgtaag accttccaca atggacaggt caggtagcca
     7021 gaacacccat caggcgcgtc ctcatcgact acgatgtaaa aatgggtctg gaaatgaact
     7081 tttcaagcct tatcagcgga cttttttttg cagtttatgg tctattgcat agactgaggg
     7141 ggcagcacga aacgttaaag tgtgatagcc gccacagagc gacgtaatga tgatgttatt
     7201 ttcagcaagg gatcgctata actcatcagg ccaacaccca cgatcaacag atgaatttat
     7261 tatgataaga aatgtgttgt tagcgttcat gatatgcagc ggaatggcat tactcggagg
     7321 atgctccagc gtgatgtccc ataccggagg gaaagaagga acgtatcccg gcacccgcgc
     7381 cagcgccacc atgattggcg atgatgaaac caactggggc actaagtcgc tggcgattct
     7441 cgatatgccg tttaccgctg tgatggacac gcttttatta ccgtgggatg tgttccgcaa
     7501 agacagctcg gtgcgctcac gcgttgaaaa aagcgaagcc aacgcgcaag ccaccaatgc
     7561 ggttattcca ccggccagaa tgcctgataa ctgatttagc gatcagtttc ggtgatccac
     7621 agctggccac cttccatcca cgccagccat tgaccatccg gcgagaagac cacggcgtcc
     7681 gcagaaggtg gattggcgtg gttttcggtt aaatactcaa cctcgccgct ttgcgcatgg
     7741 gcacaagcaa ttcgattatc cagcacaaag cccaaccatt ctcctgacgg atgccagtta
     7801 aatgcagact gaatatccgt tttgttatgg gttaactggc gcggctcgcc gccctgtggc
     7861 gagataagcc acagttgcac aatgccgtta tcatcacgca ttaaaaacgc gatttgcgta
     7921 ccctgcggat tacagcgcac ccagtggcgc gggacgttga ctaaccccgg ataagcccga
     7981 tggtgggtaa aggttaaacg tcgctgcacg acgccacgcg gtggcgcggg cagggtggtt
     8041 tccgttccac ttaacggcgc atcacctgcc gctttccagc cagcttcatc ttgcggcaac
     8101 tcaacgataa acagctccgg cactttctca ccctttggcg aaagtgtgtc gccaataaac
     8161 gccagcgcgt gatttcctac ccatccttct tcataagcac gattgatttc atcactgcca
     8221 ggctgcggcg tgggcgtggt tttactcacc agcacacacc agtggctgcc gctgtattca
     8281 cgcggatgct gcttttgtac gttgaccgga ccaaacggcg cagcaacacc gacgtttcgc
     8341 aaatccagcg ccggatcgag ttcatgcatt acatggtcgt tataggtaaa gctcaccctt
     8401 tcaccgttcg ggctaaagac atgcacatgg ctgccgccgc gcagcgctcc tggcgtatac
     8461 ggcgcggtaa tatccattgc atcgagattg ctcaccttgc cgccttcagc aatgactccg
     8521 cgccgatgat ggaagtcgta atgccatgtt tcatcaggat tttccgggcc gtgaataaag
     8581 acatatttct ctgacttcgg atgaacggtc accacgccga cgtgtgcgcc ctgtgacgca
     8641 cagtagataa cctcgacctc accggtatgg atattcacac gctcaatggt ctcgccggta
     8701 aacgacgccc cagaaggacg cacgtcaaat accagccact ggctgtcggg cgtccaggta
     8761 ttggtattgg tgagcaggtg attacgggga gcaaaggtta tctgtttcat ggcgcggtcc
     8821 tgatacaaaa cgggtccagc catcatactt cacaaggact tcactttcag acaatagtct
     8881 tttgccatcg tgatgaatgt gagtgaaaag atggaacatt tcgacgtggc gattattggc
     8941 ctcggcccgg cagggtcggc gttggcacga aagttagccg gcaaaatgca ggtgatcgcg
     9001 ctggataaaa agcaccagca tggtactgaa ggtttcagca aaccttgtgg cggtctgctg
     9061 gcaccggacg cgcagcgatc ttttattcgc gatgggctga cgcttcctgt cgatgtgatc
     9121 gctaatccgc agattttcag cgtcaaaacc gtcgacgtcg ccgcatcgct cacgcgtaac
     9181 taccagcgaa gctatatcaa tattaatcgc catgctttcg acttgtggat gaaatcgctg
     9241 atccccgcca gcgttgaggt ttatcacgac agcctgtgtc gaaaaatctg gcgtgaggat
     9301 gataaatggc atgtcatttt tcgtgcagac ggctgggagc agcatattac cgcccgctat
     9361 ctggtcggtg ccgatggtgc caactcgatg gtgcggcgac atctctaccc ggatcatcaa
     9421 atccgtaaat atgtcgcaat ccagcagtgg ttcgcagaga aacatccggt gccgttctac
     9481 tcctgcatct ttgataatgc gataactgac tgttattcat ggagtatcag caaagacggt
     9541 tattttatct ttggcggtgc ctatccaatg aaagacggtc agacgcgttt cacgactctg
     9601 aaagagaaaa tgagcgcctt tcagttccag tttggcaaag cggtgaaaag cgaaaaatgc
     9661 acggtgctgt ttccctcacg ctggcaggat tttgtctgcg gtaaggacaa cgcctttctg
     9721 attggtgaag cggcgggatt tatcagcgcc agctcgctgg aggggattag ctatgcgctg
     9781 gatagcgcag acattctgcg ttcggtgtta ctgaagcagc cagagaagct caataccgct
     9841 tactggcgcg ccacccgcaa actgcgttta aaactcttcg gcaagatagt aaaaagccga
     9901 tgcctgaccg caccggcttt aagaaagtgg attatgcgca gtggtgtggc acatattcca
     9961 cagttgaagg attagccaac gcgcttcaca tcgcccacca gcaggatata agagagcgcg
    10021 ccaatcaacg cgacggcgga gatataaacc agtgcaggtg cgaaaccgta accctgcgcc
    10081 aggtacccca ccaccagcgg aacggtgatg ccgcccagac caccggcgaa gttaaacacg
    10141 ccgccggtta aaccaatcag gcgcatcggt gccagagaag aaaccagcga ccaggtaatt
    10201 gaagcaaaac cgttaccgaa gaatgccagc gccatcaggc acataatcat catcggatcg
    10261 ttagtgtagt tagcgcccat aatgcaggtg gagatcagca agccgcagat aatcggcgtt
    10321 ttacgcgcaa agcccagtga aaagccctta cgtaccagca gatccgctac ccagccagag
    10381 agcaggacgc cgacaaacgc cgcgaggaat ggcaccgtgg tcataaagcc tgctttcagc
    10441 gccgtgattc ctttttcctg ggttaaatag ttcgggaacc aggttaagaa aaaccacagt
    10501 gtagaagcca ccgcaaattg cccaagatag acgccgatca gtttacgatg gaacaccagt
    10561 ttccagtctt tggctgttaa cggctgacgt gcctctttct tcactggcgc atcaccatcc
    10621 accagaccgc cgccatcacg aatgtaatcc agttcagctt tgctgatgcc tttggtcagg
    10681 cgcggcggct gataaacctt aaaccaaatc agcgaccaga taatgccgat accaccagtc
    10741 acaatgaaca cccagtgcca gctcaacatc tcctgaatcc agatcagcag cggagtcaaa
    10801 aacgccagac cgacaaactg accagacgta taaaaaccaa cggcggaggc gcgttcatgt
    10861 tccgggaacc agctggtcac catccggtta ttggtcggga aagcgggcgc ttcgaaaata
    10921 ccggttatcg cacgcagacc aattaatgac attaatcccg tggcaaagcc ctggaataaa
    10981 gtcgccactg accagccaaa tatcgcaata aaataagtca cgcgagaacc tacgcgatct
    11041 aaaaaccaac cgccggggat ctggcatagc gtataaagcc aggcgaaggc cgaaaataca
    11101 tagcccattt ccgctttcgt aatgccgaac tcttcctgaa tatgggcgga agccacggcc
    11161 aggttagcgc ggtcaacata acaaatgacc accgtaataa agatcatcac cagcgtcaga
    11221 taacgccgac gccccggctt tgctgcatta acgggaatat ccatagcgag ctttctccag
    11281 attttgggca tagcgaagcc gctcaccatg ccctgtaatt tacagagggt tatatttttg
    11341 tattgctgtt ttagtgcccg atgaggggct tacgtggcag gaattaccac tctgctacgc
    11401 tgttatcttc atgacgccag agtggattac gccagtccgg ggcattttta ctgaactcaa
    11461 taactttagc ttcgtcgatt tccacgccta agcccggttt cgttagcggt ttaaagaagc
    11521 cgccaaccat gctgaagtct tctttgtttt tcacaaagtc gagtaactcc gcgcctttgt
    11581 tgtaatgaat tcccatactt tgttcctgaa gtaccgcgtt ataggaaaca aagtcgatat
    11641 gcagacaagc cgccagtgca atcggtccga gcggacagtg cggcgcaagg gtcacgtcat
    11701 aggcttctgc cattccggca attttgtagc attcggtaat accgcctgca tgggagagat
    11761 ccggttgtag aatcgaaata ccgcctgcct ccagcacgcg tttaaaatcg aaacgtgaga
    11821 acatgcgctc acccgccgcc agtggaatat gcgtttgtgc cgccaatttc gggtagtatt
    11881 cggcttgttc cgccagcacc ggctcttcaa taaacagcgg gcgatacggc tccagttctt
    11941 taatcagcac tttcgccatt ggcgcgctga cgcgaccgtg gaaatcaaga ccaaactcaa
    12001 tctgattgcc aaaagcttca cgaatttgtg ccacggtgtt gactgccgca tctaccgcgc
    12061 gggagttatc aattagcccc agttcttcac aaccgttcag tttgaaggta tcgaagccga
    12121 tttcgcgcag ggttttaata ccgtcgatca catccgccgg acgatcgccg ccgacccaac
    12181 tgtaggcttt aattttgtcg cgaaccaggc cgcccatcag ttgccagacc gacgcattca
    12241 gcactttacc tttgatatcc cataacgcct ggtcgatccc ggcaatggcg ctcatcagga
    12301 ttggaccgcc acgataaaat ccggcgcgat acatcacttg ccataagtca ttgatgcgct
    12361 atggatcctg accaatcaaa tagtcaccca gctcgtgaac tgccgcttcc accgtgcggg
    12421 cgcggccttc aatcactggc tcgccccagc cgacgacgcc ttcatcggtt tcaattttca
    12481 ggaacatcca gcggggaggt aaacgatacg tggtaatttt ggtgattttc attgcactgc
    12541 ctctcgatac gccttaacaa atgctgctgc ctgctgcgcg gtgcgttcta cggactgccc
    12601 ggcgcgatag agatcgctgc ctaagcccgc cccagcacaa cctgcgtcta tccactgcgc
    12661 caggttttct ggcgtcacgc cgccaacggc aaagactgcg atgtccgatg gcaataccgc
    12721 ttttaacgct ttgatgtatt gcggaccaaa agccgatgac ggaaatattt tcagcgcctg
    12781 cgcgcccgct tcgagcgcgg taaaggcttc ggtcgccgtc gcgcagccgg ggcagacggt
    12841 catgccgtag cccaccgcac ggcggatcac ttcactatgg atattgggcg taacgatgag
    12901 ctgacagccc atcctggcga gcgcatcgac ctgttcaggt ttcagtaccg tacctgcgcc
    12961 aatcaacgcc ttgtcgccat acgcatcaac gatggcggga atgctttgct cccattgtgg
    13021 ggaattcagc gggatttcaa ccgcgtcgaa cccggcgtca atcaccgcgc caacatgcgc
    13081 cagcgcctcg tcgggcttaa tgccgcgcaa aatggcgatc agcggaagtt tagtttgcca
    13141 ctgcatgagc gatgctcctt ataccagcct gaaatgccgt gtcgcccgcc accgccgtca
    13201 cgtcgcaacc catcgcctga aaggcttgct ggtagcgcgc ggtcaacgat gttccggcga
    13261 caagggtgat ggcgtgttga tgggccacat agtcgcgcat actggcgacc tctgcgccaa
    13321 tcaacaaacc agagagaaat tcactgacct gttcacgggg aagtgttccc agcacatgcg
    13381 aggcacgaac ttcaaaaagc tgcggcaata tggcgggcgt attaagacca cgctcaaggc
    13441 cagccgtgaa ggcatcggca gagttttcct gcggcggcaa acctgcacca atcaatgagt
    13501 gatttaacag taaatgatgt aattcaccgg tcatcacggt gcgaaaatcg ttgatttgct
    13561 ggctatcggc ctgcacccat ttgcaatgcg taccgggcat gacataaaga gaggaaggag
    13621 ccagagctcg cgcgccgatc aattgagttt cttcgccgcg catcacattg tggttatcat
    13681 catgagagac gcataatccg ggaataatcc agatattgtc gccaacagac gttaattgtt
    13741 cgccaataga cgaaaaatgg gcaggaacag ataaatacgg agcaactttc cagccgacat
    13801 tgctgccgac cattcctgcc attaccactg gcgtattctc ttcacgccag tcggtcgtga
    13861 cttctgctaa caccgcagcc ggagattttc cgttcaggcg cgtgacgcct gcttctgatt
    13921 gcctgctctc caggcagtgg tcgccctgat aaagccaggc gcgcagattg gtcgatcccc
    13981 agtcaattgc gatgtagcga gctgtcatgt gatttccttt aaccttcgtg tcgagctggc
    14041 gatcatggta agcgccgcct gctctgccgc atcgccgtcc tgatgccgta tcgcatcgaa
    14101 cagcgcctta tgttcctgga gcgtttgcgg catgttggcc tcatcgccca tccaggttcg
    14161 ttcaaaaacc gcccgctgca gcgaactgat cgcaatgcta agttgctgta acaccgggtt
    14221 atgcaccgac tgcagcaccg cctcgtggta gcgaatatcc gcttcgttaa acgcttcgcg
    14281 gtcctgattg ttggcaatca tctcgttcag cgccgattca atctgcgcca gatcgctgga
    14341 ggtcgcgcgc tctgctgccc aacgggcaat cgccggttcc accagatttc gcacttcgct
    14401 catggcactg ataagccgtg ggtcgtagtc gttttccagc acccattgca gtacgtcagt
    14461 gtcgaggtaa ttccactggt tacgcggtgc cacaaacgcg ccgcgataac gtttcatttc
    14521 aatcagccgt ttcgccatca acgaacggaa cacctcacgg atgatgttgc gcgaggttgc
    14581 aaactcctca cagagttccg cctcagccgg aagcggcgag cctggtacgt atttgccgtg
    14641 aacgatctgt ttacccagcg taatgacgat gcgatcggtt ttattgagag tcatggagag
    14701 tccttgtgct ctgtatgttc ttctctactt taccccgatc gatgcataat caggaaagta
    14761 tcaatcgcag tcagacacca tcacggggct atcgcaggca aaggatagta atggaacaaa
    14821 aggctatgtt tttgtagggg aatcgctgga ttaccttatc acagatggtg ccgatgacat
    14881 cgttaagatg ctcaaggatc cagctcttaa ccggcacaat attcaggttg ctgatgacgc
    14941 aagatttgtt ttaaatgcgg ggaaaaagaa atttaccggc acaatatcac tctattacaa
    15001 ctggagcaca gaagaagaaa aagcacgagt gacacaatat cgttttgctt gtggtgttca
    15061 gcactgtacc cggacagtag aaaacctgga aggtactatc catgagaaaa ataaaaacat
    15121 ggattactca aacgtaatgg ctttctacca tccatttaaa gtgcgatttt atgaatacca
    15181 ttcacccaga ggcattccgg atggtgtttc cgcagcatta ctgccagtga ctgttacgct
    15241 ggacatcatt actgcaccgc tgcaatttct ggttgtatat gcagtaaacc aataatcagt
    15301 aagcgggcaa acgcgtttat gctgtttgcc cgcccacaga ttaattcagc acatacttct
    15361 caatagcaaa cgccacgcca tcttcaaggt tagatttggt gacaaagttc gccacttctt
    15421 tcaccgaagg aatagcgtta tccatcgcca caccgacgcc tgcatattca atcattgcga
    15481 tgtcgttttc ctgatcgcca atcgccatga tttcttccgg tttaatacct aacacgtcgg
    15541 ccagtgattt cacacccgta cctttgttaa cgcgtttatc gaggatttcg aggaagtacg
    15601 gcgcactttt cagcacggta tatttctctt tcacttcctg cggaatacgc gcgatagcct
    15661 ggtcgaggat ggcgggttca tcaatcatca tcactttcag gaactgggta ttggggtcca
    15721 ttttctccgc ttcgcagaac accagcggaa tagtggcaac gaaagattca tgcactgtgt
    15781 agtagctgat atcgcggttg gcggtgtaca gcgtggtgcg gtccagggcg tggaaatgag
    15841 aaccgacttc gcgagagagt ttttccagga aacgatagtc gtcatagctg agagcagttt
    15901 gcgccacggt gctaccatca gctgccttct gtactagcgc gccgttataa gtaatgcagt
    15961 agtcgcccgg ctgttccata tgcagctctt tcaggtagtt gtgcacacct gcatacgggc
    16021 gacccgtcgt tagcacgaca ttcacgccac gggcgcgagc tgcggcaatc gcatttttaa
    16081 cggcgggtga aatggtgtga tcgggcagca gaagggtgcc atccatatcg atagcaatga
    16141 gtttgatagc catgagttcc ccaggtagat tggttcctga cccatgctaa cgcgattccg
    16201 ctcaaaaatc agtacaacac ccgagggaaa agggggatgc aacgcgggtt gcatgctccc
    16261 catttgctta gcggaagagt ttccctttca gcagttccat acctgcggaa agcagatcgt
    16321 tattggcttg tggtgacact tcaccttgcg gtgagagcgc atcaataatc ttcggcaatt
    16381 gctctgccag taaactggaa gctgtactgg tatccacgcc aagtttttgc ccgagatcgg
    16441 acaccgcatt tgtgccgagc gccgattcca gttgctcgcc actaaccgat tgattgccct
    16501 gttgattact cagccaggtt gagagaatgg cccctaagcc gccactttgc agtttttcca
    16561 gcagcacctg aatgccgccc tgctcctcaa cccaacttaa aatagcctga tatttccccg
    16621 catcgccttt cagaaaggca ccgacaactt catcaaaaag ccccatgata atcacctgta
    16681 aagcgttacg tgttgaccca aaaagtatag atttgcggga tgataattgc ggattgcaga
    16741 aataaaaagg gcggagatga tctccgccct tttcttatag cttcttgccg gatgcggcgt
    16801 gaacgcctta tccggcctac aaaatcatga aaattcaata cattgcaaga ttttcgtagg
    16861 cctgataagc gtagcgcatc aggcacgctc gcatggttag cgccattaaa tatcgatatt
    16921 cgccgctttc agggcgttct cttcaataaa cgcacggcgc ggttcaacgg cgtcgcccat
    16981 cagcgtggtg aacaactggt cggcagcaat cgcatcttta acggtaacgc gcagcatacg
    17041 acggctttcc gggtccatgg tggtttccca cagctgttcc gggttcatct cgcccagacc
    17101 tttataacgc tggatggaga ggccgcgacg ggactctttc accagccagt ccagcgcctg
    17161 ctcgaagctg gctaccggct gacgacgctc gccacgttcg ataaacgcat cttcttccag
    17221 caagccacgc agtttctcac ccagcgtgca gatacgacga tattcgccac cggtgataaa
    17281 ctcgtgatcc agcggatagt cagtatccac accgtgggta cgcacgcgaa caatcggttc
    17341 aaacaggttt tgttcggcat tggtgtggac atcaaacttc cactggctgc cgtgctgttc
    17401 tttgtcgttc agttcgctga ccagcgcgtt cacccagcgg gtaacggtct gctcatcaga
    17461 gaggtcggct tccgtcagcg tcggctgata gataagctct ttcagcattg ctttcggata
    17521 acgacgctcc atgcggttga tcattttctg cgtcgcgttg tactcagaca ccaatttctc
    17581 taacgcttcg ccagccaatg ccggtgcgct ggcgttggtg tgcagcgttg cgccatccag
    17641 cgcgatagag atctggtact gatccatcgc ttcgtcgtct ttaatgtact gttcttgctt
    17701 gcctttcttc actttgtaca gcggcggctg agcgatgtag acgtgaccgc gttcaacgat
    17761 ttccggcatc tgacgataga aaaaggtcaa cagcagcgta cgaatgtgcg agccgtcgac
    17821 gtccgcatcg gtcatgatga tgatgctgtg ataacgcagt ttgtccgggt tgtactcgtc
    17881 acgaccgata ccacagccaa gcgcggtgat aagcgtcgcc acttcctgag aagagagcat
    17941 cttatcgaag cgcgctttct caacgttgag gattttaccc ttcagcggca gaatcgcctg
    18001 gttcttgcgg ttacgcccct gcttcgcaga gccgcccgcg gagtcccctt ccaccaggta
    18061 cagttcggaa agcgccggat cgcgttcctg gcagtctgcc agtttgcccg gcaggcccgc
    18121 taagtcgagc gcacctttac ggcgggtcat ttcacgcgcg cgacgcgccg cttcacgggc
    18181 acgggcagca tcgataattt tgccaaccac gattttcgcg tcggttgggt tttccagcag
    18241 gtattctgcc agcagttcgt tcatctgctg ttcaaccgct gatttcacct cagaagaaac
    18301 cagtttgtct ttggtctggg aggagaattt cgggtccggc actttcacgg aaacgaccgc
    18361 aatcaggcct tcacgcgcat cgtcaccggt ggcgctaact ttggcttttt tgctgtagcc
    18421 ttctttgtcc atgtaggcgt tcagggtacg ggtcatcgcc gcacggaagc ctgccaggtg
    18481 agtaccgccg tcacgctgcg gaatgttgtt ggtaaagcag tagatgtttt cctggaagcc
    18541 atcgttccac tgcaacgcca cttcgacgcc aataccgtct ttttcagtgg agaagtagaa
    18601 gatattcggg tggatcggcg ttttgttctt gttcagatat tcaacgaacg ccttgatgcc
    18661 gccttcatag tggaagtggt cttctttgcc gtcgcgcttg tcgcgcagac gaatggaaac
    18721 gccggagttg aggaacgaca actcacgcag acgtttcgcc agaatttcat attcgaactc
    18781 ggtcacattg gtgaaggttt cgaggctggg ccagaaacgc accatggtgc cggttttttc
    18841 agtctcgccg gtaaccgcca acggtgcctg cggtacaccg tgttcgtaga tctgacggtg
    18901 aattttaccc tcgcgctgga taaccagctc cagtttttgc gacagggcgt ttactaccga
    18961 aacaccaacg ccgtgcagac cgccggacac tttataggag ttatcgtcaa atttaccgcc
    19021 tgcgtgcaga acggtcatga tcacttccgc cgccgatacg ccctcttccg ggtgaatacc
    19081 ggtcggaatg ccgcgcccgt catcctgtac agagacagag ttatcggcgt gaatggtgac
    19141 gataatttct ttacagtgac ccgcgagcgc ttcgtcgata gcgttatcta ccacctcgaa
    19201 taccatgtgg tgcagaccgg tgccgtcatc cgtgtcgccg atatacatac ccgggcgctt
    19261 acgcaccgca tccagccctt tcaggacttt gatactggag gagtcataag aattcgacat
    19321 caacgtttct cgctcattta tacttgggtt aatccgttat tttacccttt tccacggtaa
    19381 acatcttcga attttcgtcc gacatgtcta taacgtgttc agcactgatc gcgctgacaa
    19441 agacctgtga ttgcgtcgct tttaagcggc tggcaagcag cccgcgacgc tcatcatcaa
    19501 gctcagaggc aaaatcatct atcaggtaga gacaccgccg cccgctttca cgggtgagga
    19561 actctccttg cgccagacgt aaggcgcaca tcaacagctt aagctgccca cgcgataagg
    19621 tatcttccac cggcgcaccg tcggcgcgaa tgcgtaagtc cgctttatgc gggccatgcg
    19681 cggtgtaggt tagctggcga tcgcgttcaa aattacgttc cagcacctca gcatattctg
    19741 tctctttctc ccagccgcgc tggaaagaga aagtcagaga aaactcaggg agaaattgct
    19801 tacaagtatc ggccatatca gccgcgatac cggcgctata ctccgcgcgc caggtgctga
    19861 tttgctccgc cagcgggatc agctctttat cccacgggcg tagctgttcg taacgtgtca
    19921 cctggcgcag cgccgcattg cgctgcttga gcaatcgctt gagattgctc caggcggtga
    19981 aaaatccggg ttcgttgtga aagcatcccc agtcgaggaa tgctcttctg tatttggggc
    20041 cgccgttgag taaagtaaac ccttctggcg ttatcaactg cattggcatc aggtgcgcca
    20101 gttccgcgac cttatgcccg tctgtaccgt cgatgcggac tttgctgtcg ccctgtttgt
    20161 ctttggttaa gccaatcgct gtctcgcgct cttcgccctg taatcgcccg tggagaacaa
    20221 acgcctcctg ctcatggcga atgacgcgac caatctgcaa actgcgaaac gcccgaccat
    20281 ggccgagcgt atagatggct tccagcacgc tggttttgcc actgccgttg gcacctacca
    20341 gaaagttaaa gccgggagat aaagcgagat ccgcggtttc aatgttgcgg aaatcgcgga
    20401 tcaacaagcg ggtgagggac attacagtct cattggcatg acaacatagg cagccgactg
    20461 tgatgcggca tcttcaatct gcacgctgga aaccgaatcg gtcagcatca tgcggacgtt
    20521 ttcacatttc agcgcgttca gaacatccag cacgtagctg acgttgaagc cgatttccat
    20581 ctccgcaccg ctataggtaa cgtcgaggat ctcttccgct tcttcctgtt ccgggttgtt
    20641 ggcggtgatt ttcagctggt tttcgctgac ataaagacgt acgccgcgga atttctcgtt
    20701 agagagaatc gccgcgcgag caaacgcctg cttgagcaga tcgcagccag cttccagatg
    20761 tttgtccggg ttcttcggca gaacgcggcg ataatccggg aagcgaccat ccaccagttt
    20821 ggaggtgaag ataaagtcgc caacgtgggc gcgaatgttg ttgctgccaa tctgtacgcg
    20881 cagcggattg tcgccgccgt cgagcatacg catcagttca atcacgcctt tacgcggcac
    20941 gatcaccgaa tggcttggca aagattgacc aattggcatt gaacagactg ccagacggtg
    21001 gccgtcggtc gccacggtgc gcagttcttc gccttcggtt tcaaacagca taccgtttaa
    21061 gtaatagcga acgtcctgat gcgccataga aaactgggtc gcttcaatca gacgcttcat
    21121 cgtcgcctgc ggcagggtaa attcgacttc gctctgccag tcatcgaggt tcgggaaatc
    21181 cgccgctggc agggtagaca gcgaaaaacg gctacgcccg gagcgcacca gcatccgttc
    21241 accttccagc tgtacggcaa tttccgcgcc ttcaggcaga ccacggcaga tatcaaagaa
    21301 tttgcgcgcc ggaacggtcg tcgctcctgg ctcgtgtggc tgaaccagcg caacgcgtgc
    21361 caccatttcc atctcgagat cagtaccggt cagcgacaac gtaccgtcag caacctgtaa
    21421 cagcagatta ccgagaatcg gtagcgtagg acgaccacct aacggcccgc tcacctgttg
    21481 tagcggtttt aataaatgct cacgttctac ggtaaatttc ataggtttac gatgacaatg
    21541 ttctgattaa atttgaaaaa tcttctttga tatcgtggct ctcttcacgc aactgctcga
    21601 tcttacggca ggcatgaagc accgtcgtgt ggtcacgacc accaaacgca tcgccaatct
    21661 ccggcagact gtggttagtc agctctttcg ccagcgccat cgccatctgg cgcggacgcg
    21721 ccaccgagcg ggatcgacgc ttggaaagga gatccgcgac tttgatcttg tagtactccg
    21781 ccaccgtctt ctgaatattg tcgatggtga ccagtttttc ctgcaatgcc agcaagtcgc
    21841 gcagtgcctc acgcacgaag tcgatggtga tcgcccgtcc ggtaaagttg gcgttagcaa
    21901 taacgcggtt cagcgcccct tccagctcac gtacgttaga tcgtagacgc ttggcgataa
    21961 agaacgccac ttcgcctggc aaacgaatgt cattttcgtc ggcctttttc atcaggatcg
    22021 ccacgcgggt ttccagctct ggcggttcga tcgccacagt cagtccccaa ccgaagcggg
    22081 atttcaaacg atcctcaacg ccgttgatct ctttcggata gcgatccgag gtgagaatga
    22141 tctgttgatt accttccagc agggcgttga aggtgtggaa aaactcttcc tgagatcgtt
    22201 ctttattagc aaaaaactga atatcgtcga tcagcagtgc atctacggaa cggtagtagc
    22261 gtttaaactc ttcgatcgcg ttgttttgca gggctttaac catgtcctga acaaagcgct
    22321 cggagtgcat ataaaccact ttggcattcg gcttgcgcgc cataatgccg ttacccaccg
    22381 catgcagcag gtgagtttta cccagacccg tgccgccata aaggaacaat gggttatagg
    22441 caccgccagg gttatccgcc acctggcgag ccgccgcgcg cgccagttgg ttagatttac
    22501 cttcaacgaa gttatcaaac gtgtgtttga cgtttacgtt agaacgatag gtcggttctg
    22561 ccggggccgg gacgttatcc caacctgagc gcgtagaagg cgcagcacgt tgcggctgcg
    22621 tttgcgccac ctgtgcaggg gccgcgacgt tgctcgtcac tgccgcttgt ggcgtttgcg
    22681 tcaccggttt ggtgccgact tcaaaacgca gctgtggggc atccgctccg cagaaactgg
    22741 ttagcagtcc attgatatta ttaaggtact tgtcccgtac ccaatcgagg acaaaacggt
    22801 ttggcgcgta cagggccagc gtgttatcgc tcagttccgc ctgcaatggg cgtatccaca
    22861 tactgaattc tgtggctggt aactcatcct gcaatcgggc aagacactgc tgccaaagcg
    22921 aaagtgacac ggcggactcc actcgaacaa aagtcgataa tgactaaggc tgaaacattc
    22981 atgattgttg acgtacgtcg aaaagaccct gcttgagggt gacgcacgaa ccgctgtctg
    23041 cggttatatg cccgatcaag atcctgcaaa acgatcggga ccgcggatca tagcctaaac
    23101 tgcgcaagag atcttctgtt tctcacagat tttcccgatt tatccacagg actttccaga
    23161 cctcgctaag tgtaaacgat cctgccgcga ggcgggcacg atttacgccg catattggaa
    23221 aatttaatga ccatagacaa aaattggctt aatcgatcta ataaagatct aggacgatcc
    23281 ttgcgcttta cccatcagcc cgtataatcc tccacccggc gcgccatgct ggttttcact
    23341 ggtgtgaggt cgtacatttt ccctgcgaaa aggtgcggaa aagcgcggta aataaggaaa
    23401 gagaattgac tccggagtgt acaattatta caatccggcc tctttaatca cccatggctt
    23461 cggtgtccat cgtttcattt ttcggcggat atccaataaa gccattgaat ttattcaagt
    23521 ttaggtagaa atcgccatga aacgcacttt tcaaccgtct gtactgaagc gcaaccgttc
    23581 tcacggcttc cgtgctcgta tggctactaa aaatggtcgt caggttctgg cacgtcgtcg
    23641 tgctaaaggc cgcgctcgtc tgaccgtttc taagtaataa agctaacccc tgagtggtta
    23701 agctcgcatt tcccagggag ttacgcttgt taactcccag tcaattcaca ttcgtcttcc
    23761 agcagccaca acgggctggc acgccgcaaa ttaccattct cggccgcctg aattcgctgg
    23821 ggcatccccg tatcggtctt acagtcgcca agaaaaacgt tcgacgcgcc catgaacgca
    23881 atcggattaa acgtctgacg cgtgaaagct tccgtctgcg ccaacatgaa ctcccggcta
    23941 tggatttcgt ggtggtggcg aaaaaagggg ttgccgacct cgataaccgt gctctctcgg
    24001 aagcgttgga aaaattatgg cgccgccact gtcgcctggc tcgcgggtcc tgatagccct
    24061 cattcgggtc tatcaacgcc tgattagtcc gctactcggg ccgcattgtc gtttcactcc
    24121 aacctgttca agctacggaa ttgaggcatt gcgcaggttt ggagtgataa aaggcagttg
    24181 gttgacggtg aaacgcgtat taaaatgcca ccctttacac cctggtggtg acgatcccgt
    24241 cccgcccgga ccatttgata ccagagaaca ctaacgatgg attcgcaacg caatctttta
    24301 gtcatcgctt tgctgttcgt gtctttcatg atctggcaag cctgggagca ggataaaaac
    24361 ccgcaacctc aggcccaaca gaccacgcag acaacgacca ccgcagcggg tagcgccgcc
    24421 gaccagggcg taccggccag tggccagggg aaactgatct cggttaagac cgacgtgctt
    24481 gatctgacca tcaacacccg tggtggtgat gttgagcaag ctctgctgcc tgcttacccg
    24541 aaagagctga actctaccca gccgttccag ctgctggaaa cttcaccgca gtttatttat
    24601 caggcacaga gcggtctgac cggtcgtgat ggcccggata acccggctaa cggcccgcgt
    24661 ccgctgtata acgttgaaaa agacgcttat gtgctggctg aaggtcaaaa cgaactgcag
    24721 gtgccgatga cgtataccga cgcggcaggc aacacgttta ccaaaacgtt tgtcctgaaa
    24781 cgtggtgatt acgctgtcaa cgtcaactac aacgtgcaga acgctggcga gaaaccgctg
    24841 gaaatctcca cctttggtca gttgaagcaa tccatcactc tgccaccgca tctcgatacc
    24901 ggaagcagca acttcgcact gcacaccttc cgcggcgcgg cgtactccac gcctgacgag
    24961 aagtacgaga aatacaagtt cgataccatt gccgataacg aaaacctgaa catctcttcg
    25021 aaaggtggtt gggtggcaat gctgcaacag tatttcgcga cggcgtggat cccgcataac
    25081 gacggtacca acaacttcta taccgctaat ctgggtaacg gcatcgccgc tatcggctat
    25141 aaatctcagc cggtactggt tcagcctggt cagactggcg cgatgaacag caccctgtgg
    25201 gttggcccgg aaatccagga caaaatggca gctgttgctc cgcacctgga tctgaccgtt
    25261 gattacggtt ggttgtggtt catctctcag ccgctgttca aactgctgaa atggatccat
    25321 agctttgtgg gtaactgggg cttctccatt atcatcatca cctttatcgt tcgtggcatc
    25381 atgtacccgc tgaccaaagc gcagtacacc tccatggcga agatgcgtat gctgcagccg
    25441 aagattcagg caatgcgtga gcgtctgggc gatgacaaac agcgtatcag ccaggaaatg
    25501 atggcgctgt acaaagctga gaaggttaac ccgctgggcg gctgcttccc gctgctgatc
    25561 cagatgccaa tcttcctggc gttgtactac atgctgatgg gttccgttga actgcgtcag
    25621 gcaccgtttg cactgtggat ccacgacctg tcggcacagg acccgtacta catcctgccg
    25681 atcctgatgg gcgtaacgat gttcttcatt cagaagatgt cgccgaccac agtgaccgac
    25741 ccgatgcagc agaagatcat gacctttatg ccggtcatct tcaccgtgtt cttcctgtgg
    25801 ttcccgtcag gtctggtgct gtactatatc gtcagcaacc tggtaaccat tattcagcag
    25861 cagctgattt accgtggtct ggaaaaacgt ggcctgcata gccgcgagaa gaaaaaatcc
    25921 tgattcggtg agttttcgct aaaataaggg cggtcagttg accgcctttt ttcttttcgt
    25981 agggtggata agcaccgcgc atccgccaca caaagcaaca ggaacatcat gagcgataat
    26041 gacactatcg tagcccaggc cacgcctccg ggacgtggcg gcgttggcat cctgcgcatc
    26101 tccggcctaa aagcccgtga agttgccgaa accgtgctgg gtaaactgcc taagccgcgc
    26161 tacgccgatt atcttccgtt taaagacgcc gacggcagcg tgctcgatca ggggattgcg
    26221 ctatggttcc ctggcccgaa ctcgttcacc ggcgaagatg tgctggaact gcagggtcat
    26281 ggcggtccgg tgatcctcga cctgctgtta aaacgcattc tgaccattcc cggcctgcgg
    26341 attgctcgcc ctggtgagtt ttccgaacgc gcgtttctta acgataaact cgacttagcc
    26401 caggccgagg cgattgccga tcttatcgac gccagttcgg aacaggcggc ccgttcggca
    26461 cttaactcgc tgcaaggcgc attctccgca cgggtcaacc atctggtgga agccctcacc
    26521 cacttgcgca tttacgtcga agcggcaatt gatttccctg atgaagagat cgatttcctc
    26581 tccgacggca aaattgaagc ccagcttaat gacgtgattg ccgatctcga tgcagtgcgt
    26641 gctgaagcac gtcagggtag tttgttgcgc gaagggatga aggtggtgat tgccggacgt
    26701 cctaacgccg gtaaatcgag cctgttaaac gcgctggcgg gccgtgaagc agcaatcgta
    26761 actgatatcg ccggaaccac acgtgacgtg ctgcgtgagc atatccacat tgacggaatg
    26821 ccgctgcata tcatcgatac cgctgggcta cgtgaagcca gtgacgaagt ggaacgtatt
    26881 ggtatcgagc gcgcgtggca ggaaattgaa caggccgacc gcgtgctgtt tatggtcgat
    26941 ggcaccacaa cagacgccgt ggatccggca gagatctggc cggaatttat cgcccgtctg
    27001 ccagcgaaac taccgatcac cgtggtgcgc aataaagccg atatcaccgg cgaaacgctg
    27061 ggaatgagtg aagtgaacgg tcacgcgtta attcgtctct cggcaaggac aggtgaaggc
    27121 gtggaggtgc tgcgtaacca tctcaaacag agcatgggct ttgacaccaa catggaaggc
    27181 ggcttcctgg cgcgtcgtcg ccacctacag gcgctggaac aggcagcgga gcatttgcag
    27241 caaggtaaag cgcaactgtt aggtgcctgg gcgggtgaac tgctggcgga agagttgcgc
    27301 ctggcacagc agaacttaag cgaaatcacc ggggaattta cctctgatga tttgctgggg
    27361 cggattttct ccagcttctg tattggtaag taaccgcgct tacgaagccg tattctgact
    27421 gtcagatgcg gcttcgcttc attgttagca ctcctgttat tcctcaaccc tttttttaaa
    27481 cattaaaatt cttacgtaat ttataatctt taaaaaaagc atttaatatt gctccc
//
LOCUS       DAJVRF010000043        27105 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJVRF010000043 DAJVRF010000000
VERSION     DAJVRF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27105
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.043"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..924
                     /locus_tag="OQN13_001676"
     CDS             <1..924
                     /locus_tag="OQN13_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1615055.1"
                     /translation="TSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKL
                     NDSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPV
                     NIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNL
                     TAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQ
                     MRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGT
                     VNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(967..1827)
                     /gene="rsmI"
                     /locus_tag="OQN13_001677"
     CDS             complement(967..1827)
                     /gene="rsmI"
                     /locus_tag="OQN13_001677"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCS1615056.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1891..3927
                     /gene="lpoA"
                     /locus_tag="OQN13_001678"
     CDS             1891..3927
                     /gene="lpoA"
                     /locus_tag="OQN13_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCS1615057.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3885..4280
                     /locus_tag="OQN13_001679"
     CDS             3885..4280
                     /locus_tag="OQN13_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCS1615058.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4300..4890
                     /gene="diaA"
                     /locus_tag="OQN13_001680"
     CDS             4300..4890
                     /gene="diaA"
                     /locus_tag="OQN13_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCS1615059.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4900..5475
                     /gene="yraP"
                     /locus_tag="OQN13_001681"
     CDS             4900..5475
                     /gene="yraP"
                     /locus_tag="OQN13_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCS1615060.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5589..6629)
                     /locus_tag="OQN13_001682"
     CDS             complement(5589..6629)
                     /locus_tag="OQN13_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1615061.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6702..7337)
                     /locus_tag="OQN13_001683"
     CDS             complement(6702..7337)
                     /locus_tag="OQN13_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCS1615062.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7465..7983
                     /gene="yhbO"
                     /locus_tag="OQN13_001684"
     CDS             7465..7983
                     /gene="yhbO"
                     /locus_tag="OQN13_001684"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCS1615063.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7963..8406)
                     /locus_tag="OQN13_001685"
     CDS             complement(7963..8406)
                     /locus_tag="OQN13_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCS1615064.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8457..8759
                     /gene="yhbQ"
                     /locus_tag="OQN13_001686"
     CDS             8457..8759
                     /gene="yhbQ"
                     /locus_tag="OQN13_001686"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCS1615065.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8746..9249)
                     /locus_tag="OQN13_001687"
     CDS             complement(8746..9249)
                     /locus_tag="OQN13_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1615066.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9243..9767)
                     /locus_tag="OQN13_001688"
     CDS             complement(9243..9767)
                     /locus_tag="OQN13_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCS1615067.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9976..10971
                     /locus_tag="OQN13_001689"
     CDS             9976..10971
                     /locus_tag="OQN13_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS1615068.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10980..11858
                     /locus_tag="OQN13_001690"
     CDS             10980..11858
                     /locus_tag="OQN13_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS1615069.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12064..13071
                     /locus_tag="OQN13_001691"
     CDS             12064..13071
                     /locus_tag="OQN13_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCS1615070.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13189..14433)
                     /gene="mtr"
                     /locus_tag="OQN13_001692"
     CDS             complement(13189..14433)
                     /gene="mtr"
                     /locus_tag="OQN13_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCS1615071.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14587..16476)
                     /gene="deaD"
                     /locus_tag="OQN13_001693"
     CDS             complement(14587..16476)
                     /gene="deaD"
                     /locus_tag="OQN13_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCS1615072.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16469..16549)
                     /gene="yrbN"
                     /locus_tag="OQN13_001694"
     CDS             complement(16469..16549)
                     /gene="yrbN"
                     /locus_tag="OQN13_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCS1615073.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16656..17540)
                     /gene="nlpI"
                     /locus_tag="OQN13_001695"
     CDS             complement(16656..17540)
                     /gene="nlpI"
                     /locus_tag="OQN13_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCS1615074.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17649..19784)
                     /gene="pnp"
                     /locus_tag="OQN13_001696"
     CDS             complement(17649..19784)
                     /gene="pnp"
                     /locus_tag="OQN13_001696"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCS1615075.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20031..20300)
                     /gene="rpsO"
                     /locus_tag="OQN13_001697"
     CDS             complement(20031..20300)
                     /gene="rpsO"
                     /locus_tag="OQN13_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCS1615076.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20449..21393)
                     /gene="truB"
                     /locus_tag="OQN13_001698"
     CDS             complement(20449..21393)
                     /gene="truB"
                     /locus_tag="OQN13_001698"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCS1615077.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21393..21794)
                     /gene="rbfA"
                     /locus_tag="OQN13_001699"
     CDS             complement(21393..21794)
                     /gene="rbfA"
                     /locus_tag="OQN13_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCS1615078.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21958..24630)
                     /gene="infB"
                     /locus_tag="OQN13_001700"
     CDS             complement(21958..24630)
                     /gene="infB"
                     /locus_tag="OQN13_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCS1615079.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24655..26142)
                     /gene="nusA"
                     /locus_tag="OQN13_001701"
     CDS             complement(24655..26142)
                     /gene="nusA"
                     /locus_tag="OQN13_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCS1615080.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26170..26622)
                     /gene="rimP"
                     /locus_tag="OQN13_001702"
     CDS             complement(26170..26622)
                     /gene="rimP"
                     /locus_tag="OQN13_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCS1615081.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26829..26905)
                     /locus_tag="OQN13_001703"
     tRNA            complement(26829..26905)
                     /locus_tag="OQN13_001703"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26869..26871),aa:Met,seq:cat)
BASE COUNT         6420 a         7518 c         6811 g         6356 t
ORIGIN      
        1 acctctgcaa cgctaagtag cactaactgg caatacgcct gttcctgctc tgcgggtaag
       61 gcagttaaac ttgtctatat ggtcagcccc gtacttacca ccactggaca tcagacagga
      121 tattacaaac tcaatgacag cctggatatt aaaaccacat tacaggcaaa cgacattcca
      181 ggactcacaa ccgaccaggt tgtctctgtt aacacccgat tcacacagat aaaaagcagc
      241 accgtatatt ctgctgcaac ccaaacgggt gtttgccagg gtgacacgtc tcgttatgga
      301 cccgttaata ttggtgcaaa taccaccttt accctgtatg tcaccaagcc atttctcggc
      361 tcgatgacca ttccgaaaac ggatattgcc gtcattaaag gcgcgtgggt cgatggaatg
      421 ggaagcccgt ctacaggtga cttccatgat ttagtcaagt tatcgattca gggaaatctc
      481 accgccccac agtcgtgcaa aattaatcag ggcgatgtta ttaaggttaa ttttggattc
      541 atcaatggtc agaagtttac cacccgcaat gccatgccag acggttttac tccagtagac
      601 tttgatatca cttatgactg tggtgatact tcaaagatta aaaactcgtt gcaaatgcgc
      661 atcgacggta caactggggt agtagaccag tacaacctgg tcgccaggcg aagaagttca
      721 gacaatgtgc ccgatgtcgg tattcgtatt gaaaatctcg gcggcggagt tgcaaatatt
      781 ccttttcaga acggtatcct tcccgttgat ccttccgggc atggcaccgt caatatgcgc
      841 gcctggccag ttaatctggt cggtggtgag ctggaaacag gaaaatttca gggcaccgcc
      901 accattaccg tcatggtgcg gtaaaagaag acgttgcgtt ttgattaacg caacgcttcg
      961 gcactgttac ccctgctgct ccagcgcata cttatacagc gcatttttct tcacgccatg
     1021 aatttctgcg gccagcgccg ccgctttttt cagcggcagt tctgcctgta gcagcgccag
     1081 cgtgcgcagg gcatcggcgg gtaagtcttc ttcctgtgct ttatgacctt cgacaatcag
     1141 caccatttcg cctttgcgac ggttttcatc ttcctttacc cacgccagca gctcgccaac
     1201 gggcgcgccg tgaatggttt cccaggtttt ggtcagctca cgcgccagaa ccacgtagcg
     1261 ggattcgcct aataccgcaa cgatatcttc caggctatct aacagacggt gggtagattc
     1321 ataaaaaatc agcgtgcgcg gctccgcttc aatggctttt agcgcatcac ggcggccttt
     1381 tgatttggca ggtaaaaagc cttcgtaaca gaaacggtca gagggtaaac ccgcggcgct
     1441 taacgcagtg atagcagcac acggtccggg tagcggcacc acacgaatcc ccgcttcacg
     1501 gcaggtacgc accagatggt agccaggatc gttaattagc ggcgttccgg catcggaaac
     1561 cagcgcaatg ttttgcccct cttgcagctt cgccagcagc gtttcggctt tttgttgttc
     1621 gttatggtcg tgcagcgcaa acaaccgggc attaatccca aaatgttgca gcaataaacc
     1681 ggtgtgacga gtatcctcgg cggcaatcag atcaacggcc tgtaatacct ctaacgcacg
     1741 ctgggtgata tccgccagat tgccgattgg cgtcggtaca atgtaaagct ggccctgaga
     1801 attatccgcc gattggtgtt gtttcattgt gtcgtccgta ttgccgattt aatattgagc
     1861 attgcgtaaa aaaatatcac tggatacatt atggtaccct caacattttc tcgtttgaaa
     1921 gccgcgcgtt gtctgcctgt tgttctggca gccctgattt tcgccggttg tggcacccat
     1981 actcccgatc agtccactgc ttatatgcag ggcacggcgc aggctgattc tgccttttat
     2041 cttcagcaga tgcagcaaag ctctgatgat accaggatca actggcaatt actcgccatt
     2101 cgtgcactgg tgaaagaagg taaaaccggg caggcggttg agttgtttaa ccaactaccg
     2161 caagaactga acgattctca gcgtcgcgag aaaacactgc tggcggtaga gattaaactg
     2221 gcgcagaaag attttgctgg cgcgcaaaac ttgctggcga aaatcacacc tgccgattta
     2281 gaacaaaacc agcaagcgcg ttactggcag gcaaaaatcg atgccagcca ggggcgtcct
     2341 tccattgatt tactgcgcgc gttaattgct caggaaccgc tgctcggcgc gaaagaaaaa
     2401 aagcagaata ttgatgccac ctggcaggcg ctctcctcca tgactcagga acaggcgaat
     2461 acgctggtga tcaacgccga cgaaaatatt ctgcaaggct ggctggatct gcagcgcgtc
     2521 tggtttgata accgtaacga tcccgacatg atgaaagccg ggatcgccga ctggcaaaaa
     2581 cgttatccga acaatccggg cgcgaaaatg ctgccaacgc agttggttaa cgtaaaagcg
     2641 tttaaaccag cctcgaccaa caaaatcgcc ctgctgttgc cgctgaatgg ccaggcagcg
     2701 gtatttggtc gcaccattca gcaaggcttt gaagcggcga aaaatatcgg cactcagcca
     2761 gtggcggctc aggtagctgc cgcacctgcc gcagacgtag ctgaacaacc tcagccgcaa
     2821 accgtggatg gcgttgccag cccggcacaa gcctcggtta gcgatctgac cggtgatcag
     2881 cctgcggccc agccggtgcc tgtaagcgcc ccggcgacaa gcaccgcagc ggtaagcgca
     2941 cccgcaaatc catccgcaga gctgaaaatc tacgatacct catcacaacc acttagccag
     3001 atcttaagcc aggttcagca ggatggcgcg agtattgtgg ttggtccgtt gctgaaaaat
     3061 aacgttgaag agttgctgaa gagcaacacc ccgctgaacg tgctggcact gaaccagcca
     3121 gagaatatcg aaaaccgcgt caatatttgt tacttcgcgc tttcacctga agacgaagcg
     3181 cgcgatgcag cgcgtcatat tcgtgaccag ggtaaacaag cgccgctggt gctgatccca
     3241 cgcagttcat tgggcgatcg cgtagccaat gcgtttgcgc aagagtggca gaaactgggc
     3301 ggcggcaccg ttctgcaaca aaaatttggt tccaccagcg aattacgcgc gggtgttaac
     3361 ggcggttctg gtatcgcttt aacgggtacc ccgattactc ccagagcgac aaccgactcc
     3421 ggcatgacga ccaacaatcc aacgctgcaa accacgccaa ccgatgacca gttcaccaat
     3481 aatggcggtc gtgtcgatgc ggtgtacatt gtggcaacgc cgggtgaaat cgcttttatc
     3541 aaaccgatga tcgccatgcg taacggtagc cagagcggtg caatgctgta cgccagctcc
     3601 cgcagcgcac aaggcaccgc aggcccggat ttccgtctgg agatggaagg tttgcagtac
     3661 agcgaaatcc cgatgctggc gggcggtaat ctgccgttaa tgcagcaggc actcagcgcg
     3721 gtgaataacg attattcact ggctcgcatg tatgcgatgg gcgtcgatgc ctggtcgctg
     3781 gcaaatcatt tctcacaaat gcgccaggtt cagggttttg aaatcaacgg taataccgga
     3841 agcctgacgg caaacccgga ttgcgtgatt aacaggaagt tatcatggct acagtaccaa
     3901 caaggtcagg tagtccccgt cagttaacca ccaaacagac cggcgatgcg tgggaagcac
     3961 aagcgcgtcg ctggctggaa ggcaaaggac tgcggtttat cgccgctaac gtgaacgagc
     4021 gtggcggcga gatcgatctg ataatgcgtg aaggccggac caccgttttt gtcgaggtac
     4081 gctatcgccg ctctgcgctt tatggcggcg cggcagccag tgtgacccgc agcaaacaac
     4141 acaaattatt acagactgcc cgcttgtggc tcgcgcgtca taatgggagt tttgatactg
     4201 tggattgccg gttcgatgtg gtagccttca ccgggaatga ggttgagtgg attaaggatg
     4261 cctttaatga ccactcataa ttaaggttta aggattagcg tgcaagaaag aattaaagct
     4321 tgcttcactg aaagcattca aactcaaatt gcggcggcag aggcgcttcc ggatgccatc
     4381 tcccgtgcag ccatgacgct ggttcagtct ctgctcaatg gcaacaaaat cctctgttgt
     4441 ggtaatggaa cttccgctgc caatgcacag cattttgctg ccagcatgat caaccgtttc
     4501 gaaacggagc ggcccagctt acctgccatt gcactaaata ctgataatgt tgtcttaacg
     4561 gcgattgcca acgatcgctt acatgatgaa gtgtatgcaa aacaggtgcg ggcgctgggt
     4621 catgcgggag atgtattgtt agccatttcc acccgtggca acagccgcga tattgttaaa
     4681 gcagttgaag ccgccgttac gcgtgatatg accattgtgg cattgaccgg ctatgacggc
     4741 ggcgagcttg caggtttgtt agggccacag gatgtggaga tccgcattcc ttcgcatcgt
     4801 agtgctcgca ttcaggaaat gcatatgctg acggtaaatt gcctgtgcga tctgatcgat
     4861 aacacgcttt tccctcacca ggatgattaa ggagaataca tgaaggcatt atcgccaatc
     4921 gcagtcctta tttccgcgct gctgttgcaa ggttgtgttg ccgctgccgt agtgggtacc
     4981 gctgctgtgg gtaccaaagc cgcaactgac ccacgcagtg tcggcaccca ggtggacgat
     5041 ggtaccctgg aagtgcgcgt gaacagcgca ttgtcgaaag acgaacagat taagaaagaa
     5101 acccgtatta atgtcacggc gtatcagggc aaagtgctgc tggttgggca gtcaccaaac
     5161 gctgaacttt cggctcgcgc caaacagatt gctatgggcg tagacggtgc caacgaagtg
     5221 tataacgaga ttcgtcaggg ccagccgatt ggtctgggcg aagcatctaa cgatacgtgg
     5281 atcaccacca aagtgcgttc gcagctctta accagcgacc tggtgaaatc gtccaacgtg
     5341 aaagtgacca ccgaaaacgg tgaagtgttc ctgatggggc tggtgactga acgtgaagcg
     5401 aaagcggcgg cagatattgc cagccgggtg agcggcgtaa agcgggtaac cacagcgttt
     5461 acgtttatta aatagcagcc ccttgtaatg cctgatgcga cgcttgccgc gtcttatcag
     5521 gcctacaacg atgcaaaccg taggtcggat aaggcgttta cgccgcatcc gacatgtttt
     5581 ccctcaaatc agaataacag cgccagcccg ccgacaatca cgcctgacac tgccaccatc
     5641 gcccccgtca gccataaggc tttcgccggg aacgctttgc gcagcataat cagtgacggc
     5701 aaactcactg ccgggagcgt catcaacaat gccagcgccg gagcggttcc catacctgcc
     5761 agcatcatcg tttgtacaat cggaatttct gctgccgtgg gaatgacaaa caagcatcct
     5821 gctaccgcca tcgccaccac ccacatcagg ctgttatcga cagcaccatc ggcatggggg
     5881 aataaccaga cgcgtgcggc acccaacacc agtactgcaa ggatgtaaac cgggatcgta
     5941 ctccagaaaa gtgtccatag cgccctgccc cagcggctaa aaaaaccgct ctgtgcttcc
     6001 ggtatgtcaa tttcgaccgg tgcctgcgtt tgcggtgttt cacgcaccca tttttgcacc
     6061 agcgtcgcaa tcagcaacac catcaccagc ccggccacca gacgaatcgc cgcaaaaccc
     6121 cagccgagga caaaccccat aaacaccagc gtcgccgggt ttaacaccgg attgcccatc
     6181 cagaatgcca gcgcaccgcc catcgatacc tgttggcgac gcattcccgc cgcgaccgga
     6241 gccgcacagc aggtacacat catgcccggc agcgaaaaca gcgttcccag cagcgtgccg
     6301 cgaaagcgcg attgtccaag cgtacgcaac aaccagtcac gcgggatcag cacctgaatc
     6361 aacgaaccga ggatcacccc cagcaccgcc gctttccata ccgcgaggaa atagatcatc
     6421 gcgtaatcca acgccgcctg ccacggatta gcatccgcct gcgcaaggat agatttaccg
     6481 atactgtggg tttcggcagc agtaaacgct ttgccgtagt aaggttgcca tttcacatac
     6541 cagagaccgg caatgacaac gagaaagaaa agcgcgggtt tccaccactg aatgggcgtt
     6601 gccgcctgag atgaagactg accagtcata gcattcccca ggaataagtt atgataattg
     6661 agcgcgtgaa tattacgctc actatcaatt cttgggaata attattcagc tcttttgcgt
     6721 aattctgaag agcttaaaat agtcacgcct tcatgctcag gccgcatcgc ctctgccagc
     6781 atgacccgcg ccacgtctct cgcatcaatg gatttccagt taccgggcag taaacgaaac
     6841 agcggcgcaa aaagcgtttc gttcatccgc tgtttgctac gatcgcccag caacatcgac
     6901 gggcgagcaa tggtcaattt cggccagttc tgggcaatta atgcttcttc catctcccct
     6961 ttgacgcggt tatagaaaaa cggcgagtgg gcattggcac ccatagcact gaccaccagc
     7021 atatgctgcg cgcccagtcg ccgcccggtt aatgcggtat ccactaccag cgtgtaatcg
     7081 gcatgaataa acgcctcttt gctccccgct tctcgccgcg tggtgccgag acaacaaaac
     7141 acaatgtcga tgggttcggt gacctgcgcc aacgcgtcac tcagttgcgg atcatgggga
     7201 ttaaacaccc cgggcatatc gcccaacgga cgtcgcgtcg gcgcggctat ggcgttaact
     7261 ttcggttcgt taatcaacat ccgcagcagg tgaccgccca ccagccccgt tgcgcctgta
     7321 atcagtacct gactcatctt cgctccttta cagaattgtc tgccttgcgc tccacggctc
     7381 atgcaccagg cttaatagac catgaggtaa ttatccccga ttgtgtgaaa ttcgcctcat
     7441 ccaatgcaac aacgcggagg aagcatgagt aagaaaattg ccgttttaat cagtgatgaa
     7501 tttgaggatt cagaatttac ttcacccgca gacgagttcc gtaaagccgg acacgaagtg
     7561 attaccattg aaaaacaagc gggtaaaacg gtgaaaggca aaaaaggaga agccagcgtg
     7621 accatcgata aatccatcga tgaagtgacg ccagcggagt ttgatgccct gctgctaccg
     7681 ggtggtcatt caccggatta tctgcgtggc gacaaccgtt ttgtcacctt tacccgtgat
     7741 tttgtgaata gcggcaaacc tgttttcgcc atctgtcacg gtccgcagct gctgatcagc
     7801 gccgatgtga ttcgggggcg caaactgacc gcagttaaac cgatcattat tgatgttaaa
     7861 aatgcgggcg cggaatttta cgatcaggaa gtcgtggttg ataaagatca gctggttacc
     7921 agccggacac cggacgatct gccagcgttt aaccgcgagg cgttacgcct gctcggtgcc
     7981 tgagtcgcgt agccaaatca tttttttacc aaagcccagc gtgttgtcgg tgaatttgat
     8041 ttcatcgagc cggatttccc acaccggggc cgacagcatt ctggcaaccg gaaagcgacg
     8101 attgtacgcc tggcgcgcga ggtcgctttc ctcaccttcc agcctgcgga tctcaccttt
     8161 aaactgcacg ccgcgaatta acgctaccgt tttcggctga ccgtttaccg ttccggcaac
     8221 cgcagcctgc ggcccactca tctgcgcgtg gcgcgttttt tcttccgtca aaatgtagaa
     8281 ggcgactttc tgcgcatcaa acagataaaa agcattagcg caccacagtt ccccttcctg
     8341 ctgcacacac caggtgacaa catgttgttt tgccaaccaa cggctgatgg tgatgagtgt
     8401 ttccattgct gctctctttt atactgtggg cctgacctta acatgcgacg cagacgatga
     8461 caccctggtt tctttacttg atccgtaccg ccgacaataa gctttatacc gggatcacca
     8521 cggatgtcga acgccgctat cagcagcacc aaagcggcaa aggggcgaaa gcactgcgcg
     8581 ggaaaggaga actgacgctg gcgttttctg cgccagtggg cgatcgttcg ctggcgttac
     8641 gggcggaata tcgcgttaag caactgacca aacggcaaaa agagcgtctg gtagcggaag
     8701 gcgcagggtt tgcagagctg ttaagcagtc tgcaaacccc ggagattaaa agcgattgaa
     8761 atgctcgtga tactcaacca gaccggttac gccattcagc gcgtcatccg ctaaacgatg
     8821 tacctggaag gcgctttcag tgcccggcca gcggcaacgc agatcgtgat gcgccgccag
     8881 ttcaaagccg aaacgactgt acagcgccgg atcgcccagc gtcaccactg cggcatagcc
     8941 gaactcatta agcgaatcga gtccttcata gaccagttgg cgtgccagcc cctgtccacg
     9001 gtatttttca tcaaccgcca acggtgccat gccgacccat tgcaggtctt cgccctgcac
     9061 atcaaccgga ctaaatgcca catagccaat gacctgacct tcgtcatctg tcgccaccag
     9121 ccccagcgtc agaaagccat cttcacgcag atcgtgaacc agcttcgctt ccgcatcact
     9181 ttcgaatgag cgacgcagca gggcatcaat acccggcgca tcaatgggaa tttctactcg
     9241 aattagcatg gttcacctac cgatgtctgt ttggtttcag gcgcggtttt cattcccgcc
     9301 tcaacaaaat ccgccagttg cagcagcatc atgcgtaagg ctttcggcat ttgctccagt
     9361 tcaatggcgt ccatcaggtt tttcacatac agccccagct ccgtatcgcc ttcaatcacc
     9421 agccgacgct ggaagaagag cgtatccgga tcttgtttac gcgccgcaat catcagcaga
     9481 tcgctggcgt cggcactaaa actcacatca gcttgcgcgt tctggctgac caccagtttg
     9541 ccattcacca ccgaggtaaa ccattgcagg tcaatatcac gcacatgaat acttaaccag
     9601 cggccttcaa gaaactccag ctcaccatca tccagcgcct gacggaattg ccagcttaag
     9661 acctgctcaa gaacctggcg ttttagcgca aatggcgtca gttttaccgg tacactcaac
     9721 agagatggcc ccaaatgcac aatacgggaa cgcagtttat ccaacacgag ttttactccc
     9781 tgtttcaaca atcatcctat tttgccatat cagaaaaata acatagcggt ataaatcaac
     9841 aattccatat gaaattgctg ctaccaccaa tacaacttta actgccttaa atcaaaaatt
     9901 gtcgcagcaa ggttaactaa aatcccagtt cgttaacatt tttgcgtttt gaaagcgcaa
     9961 ccttcaggaa aaattatgga gctgctctgc cctgccggaa atctcccggc gcttaaggcg
    10021 gccatcgaaa acggcgcaga tgctgtttat atcgggctaa aagatgatac taatgcccgt
    10081 cacttcgccg gccttaactt taccgagaaa aaattgcagg aagcggtgag ttttgtccat
    10141 caacatcgcc gcaaacttca catcgcgatt aacacttttg cgcatccgga cggttacgcc
    10201 cgttggcagc gcgccgtgga tatggcggcg cagctgggtg ccgacgcgct gatcctcgcc
    10261 gacctcgcca tgctggagta cgccgccgag cgttacccgc atattgagcg tcatgtgtca
    10321 gtgcaggctt cggcgaccaa tgaagaggcg attaactttt atcatcgcca ttttgatgtt
    10381 gcccgcgtgg tgctgccgcg cgtgttgtcg attcatcagg tgaaacagct ggcacgggtc
    10441 acacctgtcc cgctggaagt ctttgctttt ggcagcctgt gcattatgtc ggaaggtcgt
    10501 tgctatctgt cgtcgtatct gacgggtgag tcgcccaaca ccgtgggcgc gtgttctccg
    10561 gcccgtttcg tgcgctggca acaaacgccg caggggctgg aatcccgcct gaacgaagtg
    10621 ctgatcgacc gttatcagga tggcgaaaac gcaggttatc cgacgctgtg taaagggcgt
    10681 tatctggtgg acggcgagcg ctatcacgcg ctggaagaac caaccagtct caataccctg
    10741 gaactgctgc cggagttaat ggcggcgaat attgcttcgg tgaaaattga aggccgccag
    10801 cgtagcccgg cgtatgtcag ccaggtggcg aaagtctggc gtcaggctat cgaccgttgt
    10861 aaggccgatc cgcaaaactt cgtaccgcaa agcgcgtgga tggagacgct cgggtcgatg
    10921 tccgaaggca cgcagaccac ccttggcgcg tatcaccgta aatggcagtg agaagagcaa
    10981 tgaaatattc cttagggcca gtgctgtggt actggccaaa agaggcgctg gaagaatttt
    11041 atcagcaggc cgccaccagc agcgccgacg tgatttatct tggtgaagcg gtatgcagca
    11101 agcgccgggc aaccaaagtt ggcgactggc tggagatggc aaaatcgctc gccggaagcg
    11161 ggaagcagat agtgctctcc acgctggcgc tggtgcaggc atcctctgaa ctgggcgaac
    11221 tgaaacgcta tgttgagaac ggtgagtttc tgattgaagc cagcgatctc ggcgtggtga
    11281 atatgtgcgc tgaacgcaaa ctgccgttcg tcgccgggca cgcgctgaac tgctacaacg
    11341 cggtgacgct gaaaatattg ctcaaacagg gcatgatgcg ctggtgtatg ccggtggagc
    11401 tttcccgcga ctggctggtg aatctgctta atcagtgcga tgagttgggg attcgcaacc
    11461 agtttgaagt ggaagtcctg agctacggtc atctaccgct ggcctactcc gcccgctgct
    11521 ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga aacctgctgc attaagtatc
    11581 cgaacgggcg caacgtgctg tcgcaggaaa accaacaagt gtttgtactc aatggcattc
    11641 agaccatgag cggctacgtt tacaacctcg gtaacgagct gacatccatg cagggattgg
    11701 ttgatgtggt ccgcctgtca ccgcagggta ctgacacttt cgcgatgctc gacgccttcc
    11761 gcgctaatga aaatggcgct gcgccactgc cgttgacggc aaacagcgac tgtaacggct
    11821 actggcggcg gctggctggg ctggagctgc aagcctaagt aaatatgctc gtcatacttc
    11881 aagttgcatg tgctgcatct gcattcgttc accccagtca cttacttatg taagctcctg
    11941 gggattcact ctcttgtcgc cttcctgcaa ctcgaattat ttagagcaca gctcactttg
    12001 ttaacaactt taactactct ttaatgcagt attaaagatt aatcggtaac aaagtgagct
    12061 gttatgactg ataaaaccat tgcgttttcg ctactcgatc tggcccccat tcccgaaggt
    12121 tcttcagcgc gagaagcatt ctcccactct ctcgatctcg cccgtctggc tgaaaagcgc
    12181 ggctatcatc gctactggct ggcagaacac cacaatatga ctggcattgc cagtgctgcc
    12241 acgtcggtat tgattggcta tctggcggcg aataccacca cgctgcatct ggggtctggc
    12301 ggcgtgatgt tgcctaacca ctcaccgttg gtcattgccg aacagttcgg cacgctcaat
    12361 acactctatc cggggcgaat cgatttgggg ctgggtcgtg cgccaggtag tgaccaacgg
    12421 acaatgatgg cgctgcgtcg ccatatgagc ggcgatattg ataatttccc ccgcgatgtc
    12481 gcggagctgg tggactggtt tgacgcccgc gatcccaatc cgcatgtgcg cccggtacca
    12541 ggctatggcg agaaaatccc cgtgtggttg ttaggctcca gcctttacag cgcgcaactg
    12601 gcggcgcagc ttggtctgcc gttcgcgttt gcctcacact tcgcgccgga tatgttgttc
    12661 caggcgctgc atctttatcg cagcaacttc aaaccgtcgg cacgactgga aaaaccatac
    12721 gcgatggtgt gcatcaatat tatcgccgcc gacagcaacc gcgacgctga atttctattt
    12781 acctcaatgc agcaagcctt tgtgaagctg cgccgtggcg aaaccggaca actgccgccg
    12841 ccgattcaaa atatggatca gttctggtca ccgtctgagc agtatggcgt gcagcaggcg
    12901 ctgagtatgt cgttggtggg tgataaagcg aaagtgcgtc acggcttgca gtcgatcctg
    12961 cgcgaaaccg acgccgatga aattatggtt aacgggcaga ttttcgacca ccaggcgcgc
    13021 ctgcattcgt ttgaactggc gatggatgtg aaggaagagt tgttgggata gtgtgtctta
    13081 acgcgggaag ccttatccga gctggcaacg ctgtcctacg tagacctgat aagcgaagcg
    13141 catcaggcat tgtgtaggca gcagaaatgt cggataaggc accgctgatt actgatacac
    13201 cggcagcaaa ttaaagctcg ataagatatg gaccagtgcg ttgccaacgc caaacaccag
    13261 aatcagcgta atcatcggct tgccgcccca gacacggaat ttcgggctgc caaagcgttt
    13321 acgcgatgcg cgggctaaca gcgccggaac aattgccgcc cagatggtag ctgctaaacc
    13381 agcataacca atggcgtaca ggaatccgtt cgggaacagt agaccaccca caacaggcgg
    13441 ggcaaaggtc agcaatgccg ttttcaagcg ccccagggct gagtcatcga aaccaaacag
    13501 atctgccaga tagtcaaaca aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa
    13561 gtttgagaac acgaccagca gaaggtccag gctacggctg ttcagcacgc cgcttaatgc
    13621 ctgtaccagc acatcaatat taccgccctt ctctgcaata ccgataaact ccggacgcgg
    13681 gatgttaccc atcgtcgcca gcaaccagat ggtatacagc gccagtgcca tcagcgtacc
    13741 gtacaccagg catttcacga tggttttcgg atctttgccg taatacttca tcaggctcgg
    13801 cacgttaccg tgataaccaa acgatgccag acagaacggc agggtcatca gcaaatacgg
    13861 cgcataagat gcattgcttt cggcgacgtt gaacaatgtc gcaggctgca catgccccag
    13921 taggctaccg aaggtgagga agaaggtaat gactttcgcc cccagtacaa tcgccgtcat
    13981 gcggctgacg gccttagtac tcaaccacac cacaaacgct accagcagtg caaaaccaaa
    14041 acccgccgcc cgtgccggga cgtttagcga catctctgcg aaggtgtgat gcagaattga
    14101 accactggca gaaatatagg cataggtcag gatatagagc acaaaggcaa tggaaatacc
    14161 gttgaccacg ttccagcctt tgcccagcaa atctttggtg atggtgtcaa aactcgaacc
    14221 gattcggtaa ttcaggttag cttccagaat catcaagccg gaatgcagca tacagaacca
    14281 ggtaaagatc agcgccgcca ttgaccagaa aaaccacgcc ccggacatga ccactggcag
    14341 agaaaacatc cctgcgccaa taatggtgcc gccgataatc accacgccgc caagcagcga
    14401 cggtgacgtt tgggtggtgg ttagtgttgc catgagagct tctctccagt gaaaaatagt
    14461 gcgactgcgt tgttatgcat tgcactgtac cagtacacga gtacaaaaga cagaaaaaaa
    14521 gccccgatgg taaaaatcgg ggctgtatat attattttac agattgtgtt cgctgttcag
    14581 cgatgattac gcatcaccac cgaaacgacg acgaccggta gagtcatcac gacgcggagc
    14641 gcggccttca cgacgttcgc cgctaaaacg acgaccatca ccacggccac cttcacggcg
    14701 ttcaccgctg aagttacgac cgccttcacg acgttcgcca ccgaaaccac gaccaccgcc
    14761 acgacgctca ccgccagtat gcggctgtgc atcgcccagt aactgcatgt tcatcggctt
    14821 gttgagaatg cgagtgcgcg taaagtgttg cagcacttca cccggcatac ctttcggcag
    14881 ttcgatggtg gagtgagaag caaacagctt gatgttacca atgtaacggc tactgatgtc
    14941 gccttcgtta gcaatcgcac caacgatatg acgaacttca acaccatcat cgcggcccac
    15001 ttcaatgcgg tacagctgca tatcgccaac atcacgacgt tcacgacgcg gacgatcttc
    15061 acggtcacca cgcgggccac ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg
    15121 gaattcacgt ttcggacgca tcggcgcatc tggcggtacg atcagagtac gttcaccctg
    15181 tgccattttc agcagtgccg cagccagagt ttcgagatcc agctcttcac cttctgcagt
    15241 cggctgaatt ttgctcagca gtgcgcggta ttgatccaga tcgctgcttt ccagctgctg
    15301 ctgtacttta gcggcgaatt tttccagacg gcgtttgccc agcagttctg cgttcggcag
    15361 ttctacttcc ggaatagtca gcttcatagt acgttcaatg ttgcgcagca gacgacgctc
    15421 gcggttctca acgaacagca gcgcgcggcc agcacgaccc gcacgaccgg tacgaccgat
    15481 acggtgaacg taagactcag aatccatcgg gatatcgtag ttaactacca ggctgatacg
    15541 ctcaacgtcc aggccacggg ctgcaacgtc ggtcgcaatc aggatgtcca gacgaccatc
    15601 tttcaggcgt tccagtgtct gttcacgcag cgcctggttc atgtcaccgt tcagcgcggc
    15661 gctgttgtag ccgttacgct caagagcttc agccacttcc agagtcgcgt ttttggtacg
    15721 aacgaagata atcgccgcat caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt
    15781 tttgcgcata ccccagacag tccagtagct ctggctgatg tcaggacggg tagtcacgct
    15841 ggactgaatg cgcacttcct gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc
    15901 ttccggcatg gttgcagaga acagagcggt ctgatgacct tccgggatct gcgccataat
    15961 ggtttcaacg tcttcgatga agcccatgcg cagcatttcg tcagcttcat ccagaaccag
    16021 accgctcagt ttagagaggt ccagagtgcc acgtttcagg tggtccagca gacggcccgg
    16081 agtaccgaca acgatctgcg gcccctgacg cagggcgcgt aattgcacgt cataacgctg
    16141 gccgccgtac agagcaacca catttacgcc gcgcatgtgt ttagagaaat ccgtcattgc
    16201 ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc accagaatct gtggtgcttt
    16261 cagctcagga tcaagattct gcaacagagg taaagagaat gctgcagttt ttccgctccc
    16321 cgtctgggcc atacccagaa cgtcgcggcc attcagcaga tgtggaatac actctgcctg
    16381 aattggagat ggtttttcgt aacccagatc gttaagggct tcaaggatag gagccttcag
    16441 gcccagatct gcaaaagtgg tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa
    16501 tggcggccag tctacataac tcatcatgaa attgatcagc aattttcatt gaaaagtgtg
    16561 aaccggctca aagtaggtgt attaacgaac aacaacgccc tcacccgtta aggtgatggc
    16621 aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt gctggtccga ttctgccagg
    16681 tcatcttggt cctggcccag gagcgataat tccaacaatg cgtatcggtg ctcaacaaag
    16741 ttatgaacgt tgttggcaac cgccagtttg aacagtgccg tggcgctgtc caaatccccc
    16801 agacttaggt agtacttacc taaatagaag ttggtttcac tgagatgctc agcgagcgag
    16861 gtgttatccg ttgcgtccgc cttgagcctt tccattaacg tttgttcgct aatgttgccc
    16921 aagtagaact cgacaatgtt ccatccccac tgttccttat ccgatttttc gaagtgctgt
    16981 ttcaacactt ctttagcctg cttctcatcg agcttctgct cggcgagata aagccacaga
    17041 ctacggaaag gatcattggg atcgtcttga taaaacgcca gcagatcatc ttgcgctaac
    17101 ttgtcacgac cgccgtaata taatgcgatc ccgcgattca agtgcgcgta gttgtaagtt
    17161 ggatcaagct caagtacaga atcaaacgct tcataggcag catcaaaatt gcctgcctgc
    17221 gttaaatata tgcctaagta attgaatact tcaggcatat ccggtcggat tgccagcgct
    17281 tgcgaaaaat cgttacgcgc taatgccctc agaccgagac tatcatacaa cactccgcgc
    17341 tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc gactggcaag gatttgttcc
    17401 atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca atggtaccgc gaggacttca
    17461 cttttacgcc aggaagtatt actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac
    17521 cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac ggttgaatga acgtcctgtt
    17581 cccggttgct aacaaggcgt cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa
    17641 tggcaacctt actcgccctg ttcagcagcc ggagcttccg gtgctgcagc aggttgagac
    17701 tgctcagtcg cttctttaat gctcagacgg atacggccct ggcgatcaac ttctagaact
    17761 ttcaccggta cttcctgacc catctgcagg taatcggtca ctttctcaac gcgtttgtca
    17821 gcgatttgag agatgtggac cagaccttct ttaccgccgc cgatggcaac aaatgcgcca
    17881 aagtcaacga tacgggtcac tttaccagtg tagacgcggc ccacttcgat ttctgcagtg
    17941 atctcttcga tacgacgaat agcatgtttc gctttctcgc cgtcggtcgc tgcgatcttc
    18001 acagtaccgt catcttcgat ttcgatggta gtgccagttt cttcggtcag ggcacggata
    18061 acagagccgc ctttaccgat aacgtctttg atcttatccg ggttgatctt gatggtatgg
    18121 atacgcggtg cgaactcaga gatatcgcca cgcggcgcgt tgatcgcctg ttccattacg
    18181 cccaggatat gcagacgcgc acctttagcc tggttcagcg caacctgcat gatctctttg
    18241 gtgatacctt caattttgat atccatctgc agtgcagaga taccgtcgcg ggaacctgca
    18301 actttgaagt ccatatcgcc caggtgatct tcgtcgccca aaatgtcaga cagtacaacg
    18361 tagttgtcgc cttctttcac cagacccatt gcgatacccg caacggcagc tttgattggc
    18421 acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg aagccataga agaagaaccg
    18481 ttggattcgg tgatttcaga cactacacgt acggtgtacg ggaatttgtc catatccggc
    18541 atgactgcca gcacgccgcg cttcgccaga cgaccgtgac caatttcacg acgcttcgga
    18601 gaaccgacca tgccggtttc gcctacggag tacggaggga agttgtagtg gaacaggaag
    18661 gtatcggtac gttcgcccat cagttcatca agaacctgcg cgtcacgagc ggtacccagc
    18721 gttgcagtaa ccagtgcctg cgtttcaccg cgggtgaaca gcgcagaacc gtgagtacgc
    18781 ggcagcacgc cagtacgcac atccagacca cggatcatat ctttttcacg accgtcgata
    18841 cgcggttcgc ctgccagtac gcggctacga acaacgtttt tctcgatagc gtgcagaatt
    18901 tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa gcagcgtcgc gatggtttca
    18961 gatttgatga catcaacctg cgcataacgc tcttgtttgt cggtgatgcg gtaagcatca
    19021 ctcagacgag cttcagccag tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc
    19081 ggctgccagt cccaacgcgg tttaccggct tctttcacta gttcattgat gttctgaata
    19141 acaacctgct gttgttcatg accgaacact actgcgccca gcatctgatc ttcgctcagc
    19201 agttcagctt cagattcaac catcagtaca gcggcttcag taccggcaac aaccagatcc
    19261 agtttgctct ctttcagctc gtcctgagtc gggttcagta cgtactggtc attgatgtaa
    19321 cctacgcggg cagcaccaat cgggccattg aacggaatac cagacagaga cagtgctgcg
    19381 gaagcaccaa tcatcgcgac gatatccggg ttaacctgcg ggttaacaga aaccacggtg
    19441 gcgataacct gaacttcgtt gacgaagcct tccgggaaca gcgggcgaat cgggcggtca
    19501 atcagacgcg cgatcagggt ttcgccttcg cttggacgac cttcacgacg gaagaagcta
    19561 cccgggatac gaccagcagc gtaggtacgc tcctgatagt taacggtcag tgggaagaag
    19621 tcctgacctg gtttggcttt tttctgtcca acaacggtaa cgaataccgc ggtgtcatcc
    19681 atgctaacca taacagcggc agtagcctga cgagccatca tgccggtttc cagagtcacg
    19741 gtgtgttggc cgtactggaa tttacgaacg atcggattaa gcaatgtaat atcctttctc
    19801 tttcttagac agtaccttac ggcactggtg ttaatacccg atcttctgcg catcctcgcg
    19861 actaatgaca accctaaccc agctctatgt gggtaaagcc tctcattagc cgcgcgaacc
    19921 tctgcaacgg aagatcattc atagcaacaa tacattagtt tccagtgaat tgctgccgtc
    19981 agcttgaaaa aaggggccac tcaggccccc ttttctgaaa ctcgcaagaa ttagcgacgc
    20041 agacccaggc gctcgatgag ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg
    20101 agcagtttac gacgctgaga aaccatgcgc agcagaccac gacggctgtg gtgatctttt
    20161 ttgtgctctg caaagtggcc ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga
    20221 acttcggtag aaccggtgtc gtttgcgtca cgaccaaact cagaaacgat tttagctgtt
    20281 gcttcagtac ttagagacat tttaaaactc caaagtatat agaatgaaag gacgccgatc
    20341 tctaattcag cgatcccagt gtacgttacg caaagtgtta aacaatttac gcgacgttaa
    20401 gcggcagtat tctactcgta gcgacctgtt atcgcaagac ggttaacatt acgccgggta
    20461 ttcaaccacc aggcgacgag gcgcgacgcg gccttcatcg tcaatttcgc ccataccgat
    20521 aaacttgccg ttctcacctt ctgtaacgcg aaccagtcct tccagcggcg caccggatgt
    20581 acgaactggg ttaccatttt tgaagtaaac agaagacgtt aacggaagat tcaccaccgg
    20641 gtagtccgaa gctggactgt ccattggcat cagtaatgga tcaagtaact ccgcagccgg
    20701 aatatcctgc tgttcggctt gttcgaccag ttcacgcaga tgttccaggg tcaccatccg
    20761 ttcaaccgga tatttactta ccgccagacg acgcaggtaa ataacatgcg cgccacaacc
    20821 gagtttttca cccaggtcat caatgatggt gcggatataa gtgccttttg agcagtgaat
    20881 ttccagctcc agctcattgc cttcgtggcg aataaacagc aattcataaa cggtaatcgg
    20941 gcgcgcttca cgcggtactt caatgccctg acgcgcatat tcgtacagtt ttttaccctg
    21001 atatttcagt gctgaataca tcgaagggat ctgttcgata tcgccacgga aagtatccag
    21061 cgccgccgcc agttgctctg cgctaaaggt taccggacgt tcttcaacga tttgtccgtc
    21121 ggcatcagaa gtatcggtac gctgtccaag acgcgcaatg acccgatagc gtttgtcgga
    21181 gtccagcaga tactgggaaa acttcgtcgc ttccccgagg caaattggca acatgcctgt
    21241 tgccagcggg tccagcgcac cggtatgccc ggcacggttg gcgttataaa tccgtttcac
    21301 tttttgcagc gcatcattgc tggacatacc ctgcggttta tccagcaaca aaacgccgtt
    21361 aatgtcgcga ccgcgacgac gaggacgact cattagtcct ccttgctgtc gtccgggtta
    21421 acacgacgtt cttcgtcatg tttgaccacg ctggtcacca ggtttgacat gcgcatccct
    21481 tcaaccagag agttgtcgta gaagaaggtc agttccggca cgatacgcag gcgcatcgct
    21541 ttccccagca ggctgcggat gaaaccagaa gcttcttgca acgctttgat gcccgcttta
    21601 accgcgtctt catctttgtc gttgaggaac gtcacatata ctttggcata cgccaggtcg
    21661 cgagacattt cgacaccgga aacggtggtc atcatgccca ggcgaggatc tttaatttca
    21721 cgctgcagga tgagagcgat ctctttttgc atttcctgcg ctacgcgctg cgggcgacca
    21781 aattctttcg ccataataaa ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa
    21841 ttaatttcag gtggaagggc tgttcacgtt ggcctgataa gacgcgccag cgtcacatca
    21901 ggcaatccat gccggatgcg gcgtaaacgc cttatcccgc atggaaccct aaaaacctta
    21961 agcgatggta cgttggatct cgatgatttc gaatacttcg atcacatcgc cagtgcggac
    22021 gtcgttgtag ttcttaacgc cgataccaca ttccataccg ttacggactt cgttaacgtc
    22081 atctttgaag cggcgcaggg actccagctc gccttcgtag ataaccacgt tgtcacgcag
    22141 aacgcggatc gggttgtgac gtttaaccac accttcggta accatacagc ctgcgatagc
    22201 accaaatttc ggcgatttga acacgtcacg aacttccgcc agaccgataa tctgctgttt
    22261 cagttccgga gacagcatac cgctcatcgc cgctttcact tcgtcaatca ggttatagat
    22321 gacggagtag taacgcagat ccaggctttc cgcttcaatc actttacgtg cagaggcatc
    22381 agcacgtacg ttgaagccaa ccaggatggc gttggacgcc gcagccaggg tggcgtcggt
    22441 ttcggtgata ccacctacgc cagaaccgat gatcttcact ttaacttcgt cagtagacag
    22501 tttcagcaag gagtcggaga tcgcttccac agaaccctgt acgtctgcct tcaggacgat
    22561 gttcacttcg tgaacttcgc cttcggtcat gttggcgaac atgttctcga gtttagattt
    22621 ctgctgacgc gccagtttaa cttcgcggaa tttaccctga cgatacagtg caacttcacg
    22681 cgctttcttc tcgtcacgta caacggtaac ttcatcaccc gcagccggta cgccggacag
    22741 gccgaggatt tccaccggaa tggacggacc cgcttccagc acttcctgac ccagttcgtt
    22801 acgcatcgca cgaacacgac cgtattcgaa gccacacaga acgatatcgc ccttgtgcag
    22861 agtaccttca cgtaccagaa cggtagcaac cggaccacga cctttatcga ggaaggattc
    22921 gataaccgca ccgctcgcca tacctttacg taccgctttc agctccagaa cttccgcctg
    22981 cagcaggata gcgtccagca gttcatcgat gccggtaccc gctttcgcag atacgtgtac
    23041 gaactggctt tcaccgcccc actcttccgg caggatgcca tactgggaga gttcgttctt
    23101 aacacgatcc ggatcggctt ctggtttatc aatcttgttc actgcaacca ccaccggtac
    23161 ctgcgccgct ttcgcgtgct ggattgcttc gatggtctgc ggcatcacgc cgtcgtcggc
    23221 agcaacaacc aggactacga tgtccgttgc ctgcgcacca cgtgcacgca ttgaagtaaa
    23281 cgcggcgtgc cccggggtgt ccaggaaggt gatcatgccg ttttcagttt caacgtggta
    23341 tgcaccaatg tgctgggtaa tgccgcccgc ttcgccagag gcaactttcg ttgaacgaat
    23401 gtagtccagc agagaggttt taccgtggtc aacgtgaccc atgatggtca caaccggcgc
    23461 gcgcggttca gccgcagcac ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt
    23521 ttcacgacgc aggataactt tgtggcccat ctcttcagca accagctgtg cggtttcctg
    23581 atcgataacc tggttgatgg ttgccattgc gcccagtttc atcatcgctt tgatgacctg
    23641 agagccttta accgccatct tgttcgccag ttcgccaacg gtgatggttt cgccgatcac
    23701 aacgtcacgg ttaacggcct gagctggctt ctggaagcct tgctgcaggg aagaaccttt
    23761 acgttttccg cctttaccgc cacgtactgc tgcgcgtgct tcttcacgat cagcttttga
    23821 ttcagcgtgt ttgttgcctt tcttcggacg cgctgctttc gcgttacgac cacggccacg
    23881 accgccttcg acttcacgat cgctttcgtc ttctgcctgg cgagcatgtt gagaagtagt
    23941 gacgtgataa tcgctggaat cttcagtcgg ttccgcgtta tcagtccatt tgttttcttc
    24001 cgccatacga cgtgcttctt cagcaacgcg acgtgcttct tcttcgagtt tacgatgcgc
    24061 ttcttcttcg gctttacgct tgagctctgc agcttcctgc tcacggcggg ctttttcagc
    24121 ctgggcgttt ttagtcatat cgtcttgttg attgctcact ttgtcttttt ccgcagcttc
    24181 acgtttcgct tgttcagcag cttcacgctt agcttgttct gcagcctcac gttcagcttt
    24241 ttgttgcgcc tcgcgtttag ccgattcttc tgcctcacga cgggcttgct cttccgcttc
    24301 acgctgcgct tgctcttccg ctgcaaggcg ttcagcctct tgcggatcgc gtttcacaaa
    24361 ggtgcgtttc ttgcggactt cgatttgtac cgatttgctt tttccaccgg taccaggaat
    24421 gttaagggtg ctgcgtgttt tacgttgcag cgtcaatttg tccgggcctg aatttttctg
    24481 attcaggtgg tcaatcaaag tctgtttctc ttgtgcagac acagagtcgt cagcagactt
    24541 ccggatacct gcatcagcaa attgctgtac caggcgttcc acggaggtct gtcgctctgc
    24601 ggccagcgtt ttaatcgtta catctgtcat gctgttcctt cctgctacag tttattacgc
    24661 ttcgtcaccg aaccagcaaa tattacgggc agccataatc agtgctccgg ccttttcgtc
    24721 ggtcaaccct tcgatatcag ccagatcatc aatgccctgt tcggcgagat cttccagcgt
    24781 acaaacgcca cgggcggcca gtttgaatgc caaatcacga tctacccctt caaggttcag
    24841 cagatcgtca gccggtttgt tatcaccgag gctttcttcc tgggcctgtg caatggtggc
    24901 cagtgcattt ttagcacgct cgcgcagtgc ttcaacggtc ggctcatcaa ggccttcgat
    24961 ttccaacagc tctttcatcg gcacataggc cagttcttcc agcgtcgaga agccttcttc
    25021 taccagaaca gtcgcgaagt cttcgtcgat gtcgagatat ttggtgaagg tgtcgatcgc
    25081 tgcgtgcgct tccgcctgat gcttagcctg caggtcgtca acggtcatca cgttgagttc
    25141 ccaaccgctc agctgcgaag ccagacgcac gttctgaccg ttacggccaa tcgcctgcgc
    25201 caggttaccg gcttcaacgg cgatatccat ggtgtgttta tcttcatcca ccacgataga
    25261 agcaacgtct gccggtgcca ttgcgttaat cacgaactgc gccgggttat catcccacag
    25321 gacgatatcg atacgctcgc cacccagttc agtagacacc gcctgaacac gcgcgccacg
    25381 catacctacg caagcaccta ccggatcgat acgtttatcg ttggttttca ccgcgatttt
    25441 cgcacgagaa cccggatcgc gagccgctgc tttaatttca atcacttctt cgccgatttc
    25501 tggcacttca atacggaaca gttcgatcag catttccggc ttggaacgag tgacgaacag
    25561 ttgcgcgcca cgcgcttccg ggcgaacgga ataaagcacg ccacgaacgc ggtcgccagg
    25621 gcggaagttt tcacgcggca gcatatcttc acgcaggatc acggcttcag cgttgttgcc
    25681 cagatccaga gagatgttgt cgcggtttac ttttttcacc acgccggtga tgatttcacc
    25741 ttcgtgttca cggaactgat caaccaccat cgcacgttcg gcttcacgca ctttctgcac
    25801 gataacctgt tttgccgtct gggtagtgat acggtcaaag gtaacagact caatctgatc
    25861 ttcaacgtaa tcgcccaggt tcaggctttc atcttcataa cgtgcggctt caagggtgat
    25921 ttccttggtc ggctgggtga cttcatcaac aactaaccag cgacggaagg tgtcaaaatc
    25981 accgcttttg cgatcgatct gtacgcggac gtcgatctct tgttcatatt ttttctttgt
    26041 tgctgtcgcc agcgcgcttt ccaatgcttc gaaaatcttc tcgcgaggta gcgccttttc
    26101 attggatacg gcttcaacta cagccaaaat ttctttgttc atcgcgggct tttcacctca
    26161 tccagactat taaaagtggg gaaccaggtt cgccttctgg atattactca gcgcgaacac
    26221 ttcatcttta ccttcgacgg taactgtgat catttcaccg tctaccgctt tgataacgcc
    26281 ctgccattta cgacggtttt gtaccgccat acggagaacc agagtcacct cttctccgac
    26341 aaaacgggcg tagtgttcag ccgtgaacag tgggcgatcg agacccggtg aggagacttc
    26401 caggttataa gcaacggtga tgggatcttc aacatccagc acagcactta cctggtggct
    26461 cacatcagca caatcatcaa cattgatgcc atcttcacta tcaatataga tgcgcagtgt
    26521 ggatgtgcga ccgcgaataa attcgatgcc aaccagttca aaacccaggg cctcaactgg
    26581 cgcagtaatc atctctgtta atttttgctc taatgtggac aagcccaccc ccaagacata
    26641 aaaaaagggc ctaaagccca gttattctgt agtcagataa caaaaaaccc cgataaatcg
    26701 gggctttata taactgaacc ctataaccgc aactgcggtc tggagcactt tccagaagga
    26761 ttttttcaaa tcccactacg aaggccgaag tcttcacagt atatttgaaa aaggactcta
    26821 agggaaagtg gttgcggggg ccggatttga accgacgacc ttcgggttat gagcccgacg
    26881 agctaccagg ctgctccacc ccgcgcctga aacgtggcaa attctactcg ttttgggtaa
    26941 aaaatgcaaa tactgctggg atttggtgta ccgagacggg acgtaaaatc tgcaggcatt
    27001 atagtgatcc acgccacatt ttgtcaacgt ttattgctaa tcatgtgaat gaatatccag
    27061 ttcactttca tttgttgaat acttttgcct tctcctgctc cccct
//
LOCUS       DAJVRF010000044        26883 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJVRF010000044 DAJVRF010000000
VERSION     DAJVRF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26883
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.044"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            34..663
                     /locus_tag="OQN13_001704"
     CDS             34..663
                     /locus_tag="OQN13_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCS1615082.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(664..1824)
                     /gene="ybdL"
                     /locus_tag="OQN13_001705"
     CDS             complement(664..1824)
                     /gene="ybdL"
                     /locus_tag="OQN13_001705"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCS1615083.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1933..3021
                     /gene="hcxA"
                     /locus_tag="OQN13_001706"
     CDS             1933..3021
                     /gene="hcxA"
                     /locus_tag="OQN13_001706"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCS1615084.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGDLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(3031..3228)
                     /locus_tag="OQN13_001707"
     CDS             complement(3031..3228)
                     /locus_tag="OQN13_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCS1615085.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3410..5515)
                     /gene="cstA"
                     /locus_tag="OQN13_001708"
     CDS             complement(3410..5515)
                     /gene="cstA"
                     /locus_tag="OQN13_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCS1615086.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5696..6109)
                     /gene="entH"
                     /locus_tag="OQN13_001709"
     CDS             complement(5696..6109)
                     /gene="entH"
                     /locus_tag="OQN13_001709"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCS1615087.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPMSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(6112..6858)
                     /gene="entA"
                     /locus_tag="OQN13_001710"
     CDS             complement(6112..6858)
                     /gene="entA"
                     /locus_tag="OQN13_001710"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCS1615088.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6858..7715)
                     /gene="entB"
                     /locus_tag="OQN13_001711"
     CDS             complement(6858..7715)
                     /gene="entB"
                     /locus_tag="OQN13_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCS1615089.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7729..9339)
                     /gene="entE"
                     /locus_tag="OQN13_001712"
     CDS             complement(7729..9339)
                     /gene="entE"
                     /locus_tag="OQN13_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCS1615090.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9349..10524)
                     /gene="entC"
                     /locus_tag="OQN13_001713"
     CDS             complement(9349..10524)
                     /gene="entC"
                     /locus_tag="OQN13_001713"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCS1615091.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            10899..11864
                     /gene="fepB"
                     /locus_tag="OQN13_001714"
     CDS             10899..11864
                     /gene="fepB"
                     /locus_tag="OQN13_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCS1615092.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(11945..13195)
                     /gene="entS"
                     /locus_tag="OQN13_001715"
     CDS             complement(11945..13195)
                     /gene="entS"
                     /locus_tag="OQN13_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCS1615093.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            13306..14310
                     /gene="fepD"
                     /locus_tag="OQN13_001716"
     CDS             13306..14310
                     /gene="fepD"
                     /locus_tag="OQN13_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCS1615094.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            14307..15299
                     /gene="fepG"
                     /locus_tag="OQN13_001717"
     CDS             14307..15299
                     /gene="fepG"
                     /locus_tag="OQN13_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCS1615095.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            15296..16111
                     /gene="fepC"
                     /locus_tag="OQN13_001718"
     CDS             15296..16111
                     /gene="fepC"
                     /locus_tag="OQN13_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1615096.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(16108..17241)
                     /gene="fepE"
                     /locus_tag="OQN13_001719"
     CDS             complement(16108..17241)
                     /gene="fepE"
                     /locus_tag="OQN13_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCS1615097.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(17457..21338)
                     /gene="entF"
                     /locus_tag="OQN13_001720"
     CDS             complement(17457..21338)
                     /gene="entF"
                     /locus_tag="OQN13_001720"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCS1615098.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(21335..21553)
                     /gene="ybdZ"
                     /locus_tag="OQN13_001721"
     CDS             complement(21335..21553)
                     /gene="ybdZ"
                     /locus_tag="OQN13_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCS1615099.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(21556..22758)
                     /gene="fes"
                     /locus_tag="OQN13_001722"
     CDS             complement(21556..22758)
                     /gene="fes"
                     /locus_tag="OQN13_001722"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCS1615100.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            23001..25241
                     /gene="fepA"
                     /locus_tag="OQN13_001723"
     CDS             23001..25241
                     /gene="fepA"
                     /locus_tag="OQN13_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCS1615101.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            25416..26036
                     /gene="entD"
                     /locus_tag="OQN13_001724"
     CDS             25416..26036
                     /gene="entD"
                     /locus_tag="OQN13_001724"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCS1615102.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(26158..26310)
                     /gene="hokE"
                     /locus_tag="OQN13_001725"
     CDS             complement(26158..26310)
                     /gene="hokE"
                     /locus_tag="OQN13_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCS1615103.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(26661..26813)
                     /gene="hokE"
                     /locus_tag="OQN13_001726"
     CDS             complement(26661..26813)
                     /gene="hokE"
                     /locus_tag="OQN13_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCS1615104.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
BASE COUNT         6415 a         7561 c         7005 g         5902 t
ORIGIN      
        1 ttataacgaa cggataaaag gaaaacgtca ggaatggggg atactatgca acaacgatta
       61 acgcaggatt taacgcaatt tctcgccagc ttgccggaag atgatcgcat caaagcgatt
      121 aatgaaatcc gcatggcgat ccatcaggtt agcccctttc gtgaagagcc agtggattgc
      181 gttctgtggg ttaaaaacag ccaacttatg cctaatgatt acaacccaaa caacgtggca
      241 ccacccgaaa aaaaactgct gcaaaaatcc attgaaatag atggttttac ccaacccatt
      301 gtggtcacac atacggataa aaatgccatg gaaatcgtgg atggttttca ccgccatgaa
      361 attggtaaag gctcatcatc attaaaatta cgcttaaaag gttatttgcc ggtgacctgt
      421 ctggaaggta cgcgtaatca acgcatcgcc gcgactattc gtcataatcg cgctcgtgga
      481 cgccatcaaa tcaccgccat gtcggaaatc gtccgtgaac ttagccagtt gggatgggac
      541 gataataaaa ttggcaaaga gctgggaatg gacagcgacg aagtgctgcg cctgaagcaa
      601 attaacggcc tgcaagagct gtttgccgac cgtcaatatt cccgcgcctg gacggttaaa
      661 taactaaagc tggcgcaggc gttcagctgc tgccagcaac gtcgattcct tcttggcaaa
      721 acagagacga atcagtttat gtgggaaggg atcggcgcaa aacaccgaca gcggaatcgc
      781 cgctacgccg tgctcctgcg tcagccactg gcaaaactca acatcatcca gggtagaaac
      841 cgcgctgtaa tccaccagca aaaagtatgt accttcacac ggtaaaatct ccagccggct
      901 ttcatttaag gcatttacca gaatatcgcg cttctggcga taaaagtccg gtaacgcaag
      961 ataatgctca ggttctgcac gtagcatatc agcaagcgcc agctgtgccg gggtattcac
     1021 cgaaaaggtc agatactgat gtaccttgcg aatttcggcg ctgatgggcg ctggcgcaac
     1081 acaataaccc actttccagc cggtcatatg ataggtcttg ccaaatgaag aaaccgccac
     1141 tgcccgctca cgcagctgcg aatgcgccag cacactggca tggccctgtt gtgaaaagtt
     1201 gatgtgctcg tagacttcat cgctaatgac aaaaatctcg tgcccggcga tcgcctgcca
     1261 caaagcggcg aaatcagcct gctgccagac agttgcactg gagttatgcg gagtgttgag
     1321 gatcaccagt ctggtgcgct cgcttaataa tgcggcaaat tcctgccagt caacgcgaaa
     1381 atgcggtggt tgcagtgcca tacgcttcac tattccccca gaaagcgcga tggcgggggc
     1441 gtaactgtca tagctgggat caaaacaaat cacttcatcg ccattgcgca ccagtgcggt
     1501 aatcgccgcg tataacgctt ccgtcgcccc tgccgttacg gtgatatcgc tatcggcatc
     1561 tggttgatag ccatacaaac gttccgtttt ctgagcaatc gcctcgcgca aggcctgcac
     1621 gccggtcatg ggcgcgtatt ggtttgcccc ctgggcaacg tggtacgcca gccgctcctg
     1681 taaatagcgc ggaccatcaa aatcaggaaa gccttgcgac aggttaatcg cctggtgttg
     1741 ctgcgccagc gcgctcatct gggtgaaaat agtggtgcca agttgtggaa gtttgctttg
     1801 tggaatcaga gggttatttg tcatttttat tgtacctgac gaagttgcgt gttgaaggca
     1861 ctataacacg atgctagtat ttggcaatca agacgtttag atgtctaaat aaaacaataa
     1921 ggacaacaca acatgcctca caatcctatc cgcgtggtcg tcggcccggc taactacttt
     1981 tcacatccag gaagtttcaa tcacctgaac gattttttca ctgatgaaca actttctcgc
     2041 gcggtgtgga tctacggcaa acgcgccatt gctgcggcgc aaaccaaact tccgccagcg
     2101 tttggacttc caggggcaaa gcatattttg tttcgtggtc attgcagcga aagcgatgta
     2161 cagcaactgg cggctgagtc cggtgacgat cgcagcgtgg tgattggcgt cggcggcggc
     2221 gcactgctcg acaccgcgaa agccctcgcc cgccgtctcg gtctgccatt tgttgccgtt
     2281 ccgacgatcg ccgctacctg cgccgcctgg acaccgctct ccgtctggta taacgatgcc
     2341 ggacaggcgc tgcattatga gattttcgac gacgccaatt ttatggtgct ggtggaacca
     2401 gagattatcc tcaacgcgcc gcaacaatat ctgctggcgg ggatcggtga cacgctggcg
     2461 aaatggtatg aagcggtggt gctggctccg caaccagaaa cgttgccgct aaccgtgcga
     2521 ctggggatca ataatgcgca ggccattcgc gacgtattgt taaacagtag cgaacaggcg
     2581 ctgagcgatc agcaaaatca acagttaacg caatcatttt gcgatgtggt ggatgctatt
     2641 attgctggtg gtgggatggt cggtgatctg ggcgatcgtt ttacgcgtgt ggcggcggct
     2701 catgccgtgc ataacggtct gactgtcctg ccgcaaaccg agaaatttct ccacggcacc
     2761 aaagtcgctt atggcattct ggtacaaagc gccttgctag gtcaggatga tgtgctggcg
     2821 caattaaccg gggcgtatca gcgttttcat ctgccgacca cgctggcgga gctggaaatg
     2881 gatatcaata atcatgcgga gatcgacaaa gtgattaccc acaccctgcg tccggtggaa
     2941 tacattcatt acctgccagt cacgctgaca ccagatacgt tgcgtgcagc gttcgaaaaa
     3001 gtggaatcgt ttaaagcctg acgtgcaaat ttagcagcag cgcgcgccgc cttttccacc
     3061 gtagcgcgcg tcctgccgct cacggaaaaa ctcttcgtag gtcatcggcg tttgatcggg
     3121 atggttaacc cgcatatgtt cgacatagtt gtcgtaatca ggcataccaa tcatcagctt
     3181 cgccgcctga cctaaatatt ttccggcttt tgccagtgaa tcaaacatct tggcctccgc
     3241 taaccatgtt gctgaaagag tgccggatgc ggcgtgaacg ccttatccgg cctacaaatg
     3301 ctatcaacgc gctcggtcag tcccctcgcc ccatcgggga gagggttagg gtgaggagga
     3361 accctcggca ctatgccaac attcgccaac atcccccctc actctgactt tagtgtgcgc
     3421 cttttgcctg cgccacgatc tcctcgacat tttccggcat tggctcatac ggcgtttctt
     3481 tcgccgttgg cttcggatct ttcaatgccg ccagtgccga cttaatcgag aacagcgcca
     3541 gaaccaccac gaccaccata aagaagatgg ttaacccggc atccagacgg ttgttgaaca
     3601 ccagttgcgc cagctgtgac tcagtatact gcgacggaat attgccgctg tctatcattg
     3661 cctggaactt attagcaatg gccaggaagc cgactttcgc atccgggcta aacgctttct
     3721 gccagcctgc ggtcagggta caaatcagca gccaggccgt tggtaccagc gccacccagg
     3781 cgtaacgttg acgtttcatc ttgaacaaca ccacggcaca gagcatcagc gccatccctg
     3841 ccagcatctg gttggcaata ccaaacagcg gccacagagt gttaatgccg cccagcggat
     3901 cgaccacgcc ctgatggagg aagtagcccc acgccagtac gcacagcgct gttgccagca
     3961 ggttagcagg cagtgaatcg gtccgcttca ggccaggaga caccacgccc agcagatcct
     4021 gcaacataaa gcgcgcagca cgcgtacccg catctaccgc cgtcagaata aacagagctt
     4081 caaacagaat ggcgaagtga taccagaacg ccacatccat cataccaccc agcgcaccgt
     4141 gcaggatgta agccatcccc accgccagcg tcggcgcacc gcccgcacgg gaaatgatcg
     4201 actgttcacc cacttcgctg gcaatctggt tgagcgtatc cggagtaatc gcaaagcccc
     4261 agctactcac cacctgcgcg gcagaagcga ccacatccgc cgtccctgcc ggagccagca
     4321 ccgccatcgg gctgttcatg gcgaagtaca cgcccggatc gatgatacag gcggaaacca
     4381 gtgccataat cgccacgaag gattccatta acattccacc gtagccgata aagcacgcct
     4441 gcccttcgtt cgccagcatc ttcggcgtgg tgccggaaga gatcagcgca tggaagcctg
     4501 acaccgcgcc acaggcgatg gtgataaaca ggaacgggaa cagattaccg gtccataccg
     4561 ggccagtgcc atcgacaaat ttggtcagcg caggcatggt cagcgtcgga cgcataatca
     4621 aaatgcctac cgccagacca acgatagtcc cgattttcag gaaggtagag aggtagtcac
     4681 gcggggccag cagtaaccac accggcagca ccgccgccac aaaaccgtag cccaccagca
     4741 tccaggtcag ctgcacgccg gtaaagtcaa agtacggtgc ccaggtcggg ctttctgcca
     4801 cccagccgcc agagataatg gcaaaaataa ggaacaccag gccaatcact gacacttcgc
     4861 caatacgccc cggacgcaga tagcgcaggt agattcccat aaaaatcgcc agcggaatgg
     4921 tgaacgcaac ggtatacgtt ccccacgggc tatgagtcag ggctttcacc acgatcatcg
     4981 ccagtactgc aaggataatg accatgatca taaagcaggc taccagcgca atcaccccgg
     5041 cagttggccc catctcttct ttgaccagct cacccagcga gcgaccgtca cggcgcgtag
     5101 aaacaaacag caccatgaaa tcctgcaccg caccggcgag aaccaccccg gcgagcagcc
     5161 agatcatccc cggcaggtag cccatttgcg ctgccagcac cggccccacc agcggccctg
     5221 ctccggcaat ggccgcaaaa tggtgaccga acagcacttt cttgtccgtc ggcacatagt
     5281 ccagcccgtc gttatggcgc accgctggcg tcatacgcgt cgggtcaacc gccagcacat
     5341 ttttggcgat atacagccca taaaaacggt aagcgatcag atagatacag accgacgcca
     5401 ccacaatcca cagcgcgttg atctgttccc cacgatttaa agcaatgtat cccagagcaa
     5461 atgctcccat tacagagagc actgtccaga cgaggtattt ccctgatttg ttcatagttg
     5521 ttatccgtgt gcgtgtccat agagatgtta cattttgttt ctataacatc tttgtacgat
     5581 ttaacaacac ggtaaccatg taaatcggag ttgaaaccaa tatttaactt aacaatgtta
     5641 actcgatcac tccgttaacc gagttttcct gcaatctcat ttaattctgt cgggctcatc
     5701 ccaaaactgc cgtacccagc cgacaagtgc agcaacgccg cccctgttca tcgaaaacga
     5761 tgatttccca gctctgattt tgccgcccaa gatgcagcgg ctggcagacg ccgcgtacct
     5821 tgccttcaga catcgggcga tgatgggtgg cgttaagttc cgtacccacc acacactgcc
     5881 cgtcacgggt catcataaat ccggccatcg atcccagcgt ttcagccagc gccgccgacg
     5941 cgccgccatg cagcaggcca aacggctgat gagtacgggt atcaaccggc atttcggctt
     6001 ccagcacatc atctcccaga cgggtataca caattcccag atgcgccacc attgtgttat
     6061 cgctggtggc gttcagttcg tcgagcgtta aatggcgttt ccagatcatg cttatgcccc
     6121 cagcgttgag ccgccatcga ccacaatatc ctgtagggtg atatggctgg cgaggtcaga
     6181 ggcgaggaac aaaatcgtgt tggcgatctc ttgtggacgg gcgattttcc ccagcggaat
     6241 gccgagttta aactgctcgc caaagccgcg aatacgctgt tcttcggcgt catcgctcac
     6301 ccacagcgtg cgttgcatat cggtgtcggt ggagccaggc gaaaccacat tacagcgcac
     6361 gccgctaacc gccagttcca gcccgacgct caacgccagg cttttcagcg ccgctttcga
     6421 tgcgccataa gcactcatac caatacgcgg cgtgtgtgcg gcgtcggacg ccacagtgac
     6481 aatcgccccg ccccgctgac ggcgaaactg gttcatggtt tgctgaaaca ggttaaacgc
     6541 accgccgacg ttaaccgcaa aagtctgctg ccagtcctct ttactgagct gatcggtcgc
     6601 gcccatgcgc aaaattcccg ccgcattgac cagcacgtcc agtcgctccg tttcagctaa
     6661 cagtcgctga catacctgcg cgacctgcgc agcatcggca acatccatca cttcggtcgc
     6721 aaagggatat tgctcctgag cgaacgcttg atcaaaaccg gtaactttcg ctcccgcttc
     6781 aacaaacgcc agcgccgtgg cgtagccgat acctttacct gcgccggtta cccagacatt
     6841 tttaccgctg aaatccatta tttcacctcg cgggagagta gcttccacca ggcgtcgatg
     6901 gtcgggtttt tcgccagcat gacaaagtcg atatcgccat gcactttgcg ccagcgcgcc
     6961 gccagcgcca tcatgcgcac cgaatccaga ccgtagtcga tcaggttgtc gtcatcgaac
     7021 ggttcatcgg actcgtccag caacggcagg ataacctcac gcagcgccgc tttcctggcg
     7081 gggacaggtg ctggcagtaa tccttcagtc atcaccaccc ggccagaccg cccggccaca
     7141 tatttcagcg acatcaaatg ctcgtcacgg ctgaaatcgg ccagcgcatc cgccaccata
     7201 aacggtttaa tatcgcgcat aaatgcgtcg gttgcggtgg tcatacagcc aatgtgggca
     7261 tataccccgg taataatcag ctggttacgt ccactctctt tgagcatttg ctccagcgga
     7321 gaacgatgaa acgcgctgta gcgccacttc accagcaccg tgtcgtcggc atctggcgtc
     7381 aggcgatcca ccaccttttg ctgttccggc gaacgggtca ggcccggccc ccacatatca
     7441 ttcaacagcg cccgatcttc atcgctctgc tctttcggct gggcggtgta ataaaccggg
     7501 atattgtgct gtttgcagta gtcgcgcagc gcagcaatat tcgcgatcac ctgctccatc
     7561 atcgggcagt tctcgcccca gaagctgaca aaatagtcct gcatatcatg gattaacaac
     7621 gcggcacgtt gcggttcaaa ggcccagtca actttattct gcggaatatc gtgagactcc
     7681 ggcagtgcgt aagcctgtaa ttttggaata gccatcgtgt tctctccttc aggctgatgc
     7741 gcgtgacgcc agccactgac gtaattgttt tttatcgact ttcccgaccg ccgtcagcgg
     7801 aagtgaatcc acacactcca cgcgatccgg taatttaaat tcggcaatac cctgttcacg
     7861 caggaaacga cgcacctgca ccgcgcgcag cggctctttt accaccagat aagcacagct
     7921 tttttcgccc atcagctcat cttccatgct caccagtgcg gcatagatca ccgccggatg
     7981 gcgcagcagc aggttttcga tctcttcggc agcgatcttc tcgccgccac ggttgatctg
     8041 atctttctcg cgcccctgca cggtgatgta accctctgga tcaatagaga tcagatcgcc
     8101 ggaacagtaa aaaccgttgg catcaaaggc actggcattg tgctgcggac ttttgtaata
     8161 accgcggaag gtgtacggcc cgcgcgtcat caggcgtcca acttcccctt gcggcagtgg
     8221 atttccttca gcatcagcaa cccatacttc atcgtccgga cacattgggt aaccctgggt
     8281 atggataatt ttctccgcgc tatcatcaag tcgggtgtag ttcaccagcc cttccgccat
     8341 gccaaacacc tgctgcaact ggcagccaat ctcagcggaa atacgcgccg caagggtggc
     8401 agaaagacgt gcgccgccga cctgtaacag tttcagcgag gcaagctgcg cccggctttc
     8461 gccttcagcc agcgcctgca accacaggct aactgccggc ggcaccagcg cggtaacgtt
     8521 aacctgatgt tttgcaatca acgggaagca aagcgtggcg ctgggatcgg ccgccagaac
     8581 aaccgttcct ccggcaagaa agacgcccag cgatcctggc gaactcatag cgtagttatg
     8641 agccgccggg atcgcgcaca ggtagcgtgt ctgttgtgtg aactgacaaa tctcgacgct
     8701 acgacgcacg ctgtagtagt agtcgttatg agtgcgcggg atcagcttcg gtgtaccggt
     8761 ggtgccgccg gaaagctgga aataggccac ttcatcagca ggtgatggcg tagcagtaaa
     8821 accgtctgcc ggatggttaa tcgcatcctg caagttatgc tcaccgctgt cgttgagcag
     8881 ttgcaccacg cgaatggagg aatgttctgt gacgaaagta ttgaggaaat catccccgct
     8941 aaacagcgca tgttggcgat cggcaatcag taacgccggt tcaatctggc tggcataggc
     9001 gttcagttca ctacgctgat ggctgaacaa cgccagcacc ggcgcaacgc ccagtttcag
     9061 cagtgcgaaa aaggtaatgt aaagttcagc gacgttaccc agttgtacca gcgcggtttc
     9121 accgggttta atgccctgac ggcgtaaact acacgcgagg ttatccgccg cctgattcag
     9181 ctcccggtaa ctcaactgtc gctcgccgtc gataaccgcg atgctgtcac tcgcagcgtg
     9241 gcgagtcaga atgtcggtca gcggcaaatc ctgccagtag cctttttccc gatagcgacg
     9301 ggcaaactct tccggccagc gggtgaatgg aatgctcatc ctcgctcctt aatgcaatcc
     9361 aaaaacgttc aacatggtag aaagtttgac gcctgtttcg cgccactcac ccaacggtga
     9421 cgacgcaggc acaatccccg ctccggcaaa cagacgcacc tgattttccc gcagcttcgc
     9481 gcagcggatg gtcaccaccc attcgccgtt accttcgctg tcacaccaac ccacaatgcc
     9541 gccaaacagt tcgcggtcaa acggctccag ttcagcaata acctgggtcg cggcctgatg
     9601 cgggaaaccg ctcagcgcgg gggtcggatg cagcagacag gccagagtca gtgcgttttc
     9661 ttgcgaattc gctttacctt caaagggagt tgcgagatgc cacagcgtcg gcgtggtaat
     9721 caattgtgga gaggagggaa cgtgtaactc actactgcgt tcgcgcagta cctctttcat
     9781 cgcctgagtc accagttcat gttcatggcg atctttttct gacgccagca gacgattacc
     9841 cgcttcgcga tcgagcactt catccggctg acgacgcgcg gaaccggcta acggaatgga
     9901 gctaaaacgc tcgccgtctt tacgtagcag cagttccggg ctggccccca gcaggacgcc
     9961 accatcagcc agcgggacat ggaagttgta actaaccggg ttttgcgcaa tcaaccgttc
    10021 cagcaatacg ccactatcaa tggcggcgtc agtggtgata tcaatcaatc gtgacaacac
    10081 cactttgtcg acctgcggcg tggcggtaag tgcggcggcg cgggcaacca tctgttcaaa
    10141 cgtggtttgt tcaggaattg cctggcgttc caccacgttc agcgactggc tgcgggtaaa
    10201 acagtgggct gaggtctgtt tttcctgacg ggagaacgac tgccaggatt cgggaatata
    10261 cagcgacgaa ggctgacgtg gatcgaaggg aatcgccccg accatcaccg gatttttgat
    10321 gccctgcgct ttggcatcgg caaacagcgc ggcgagtttt tgctggaagg gactgtcggg
    10381 cgaatccccg ttcacagccg gttcatcgaa gcgggcgaaa catcctgacg tcgtaaaact
    10441 gcggtacggc gacataaaga aaaagcgatt gggcgcaagt gttgccatgg tctgctgtac
    10501 ttcctcagcc agtgacgtat ccatatcatc ctccacaaaa tgataaaggc tttaataatg
    10561 attatcattt atattttcgg tcgctaacct aaaagcaaac gcgcactatg tcaactcttg
    10621 aggtaacgca atctgcaact cggggttgca tctggttgtg gttacaatga aaatgagaag
    10681 cattattgat gaattcgcat aagcgcaatg tgatgtcctg cgccgttttg ccccctctcc
    10741 cttccagggt gagggctggg gtgagggtta atgttcgcac cagtgctggc tgttcccctc
    10801 accctaaccc tctccccaaa ggggcgaggg gacggattgt gcgctttgtc gaatttgtca
    10861 ttacgccctt aaccttatta ataacaggaa gctgacttgt gagactcgcc ccgctctacc
    10921 gcaacgccct tctattaaca ggacttttgc tttcaggaat agccgtagtt caggccgccg
    10981 actggccgcg tcagattact gacagccggg gcactcatac cctggaaagc cagccgcagc
    11041 gtattgtttc caccagcgtc accctgaccg gatcactgct ggcgattgat gctccggtga
    11101 tcgccagcgg cgcgaccacg ccgaataacc gcgtcgcaga tgaccagggc tttttacgcc
    11161 agtggagcaa ggtggcgaaa gaacgcaaac tgcaacggct ctatatcggc gaaccgagcg
    11221 ccgaagccgt tgccgcgcaa atgccggatc tgattttaat tagcgcaacc ggcggggatt
    11281 cggcgctggc gctgtatgat cagctttcca ccatcgcccc gacattaatc atcaattacg
    11341 acgacaaaag ctggcagtcg ctgttaacgc agcttggcga aattaccggg catgagaaac
    11401 aagcggcaga gcggattgca cagtttgata agcaactggc agcggcgaaa gagcaaatca
    11461 aattaccgcc gcagccggtc actgccattg tctataccgc cgctgcacac agtgccaatc
    11521 tctggacgcc ggaatcagca caagggcaga tgctggaaca actcggcttt acgctggcga
    11581 agttgcccgc agggttaaac gccagccaaa gccagggcaa acgccatgac atcattcagc
    11641 ttggtgggga aaatctggct gctgggttaa acggtgaatc gctattcctg ttcgccggtg
    11701 atcagaaaga cgccgatgcg atttatgcca atccactgct cgcgcacctg cctgcagtac
    11761 aaaacaagca ggtttatacg ctgggaaccg agacgttccg cctggattac tacagcgcca
    11821 tgcaagtgct ggacagactt aattcactgt taaaaaaaga ttgataaaac atacaattag
    11881 agcactaata atcccctcgc ccctttgggg agagggttag ggtgagggga aaatgcacct
    11941 ttagctaact gtcagacgct gtcacctgcg gcggcgtctg gcgaaaacgt cgcaactcca
    12001 ccagcaccag caataacaac acgccgataa tcaacaaacc aaaaccgctc gcgcttgcgg
    12061 aagcaaccgg tgtcatcatc gcgcccaaac cacccaacag cgccgcgcct atggcatcgc
    12121 ccgtcacgtt ctgcgccgtc cacaaaccgt taatccgccc taacatcgct tccggggttt
    12181 gcgtttgcag cattgtatat tgcagcaacg aactaaccgc gctcagccag ccgaacagcg
    12241 ccagacaaac cacgcctaaa atccacatcg gcatcaggcc aaacagacca atggcgagga
    12301 acgatcccag cgtggagagc agcatcaata accctggtcg cgcactatgt gccagcttcc
    12361 cgctggttaa cgcaccaata gccgcaccga gcgggatcgc cgcatagaga aaaccaatct
    12421 gtgccgccga catctgccag ttgtcagcca gcgccggata cagtacccgc accgcgctcg
    12481 ccatcgtcaa taaaccaccc agcaacgcaa tccctcctac cagcgggctg gcgagcagaa
    12541 aacgaaatcc tgccagtaat gatttcaacg gatgctcgcg cggctgcggt ggcggtggca
    12601 acgccggaag gcttaacaac ggtagcaagg taataaacgt gcccgccgcc gccagcccgt
    12661 agttccaggc tacgccgccg gtcgccagca ataaaccgcc aatcatgggc gaaatcaccg
    12721 accccagacg cacggtcaac atggtgatcg ccccggcctg cattaagttt tcacgcccta
    12781 ccagtgcagg tgttgccgcc agtagcgccg taacaccaag tgatgcgaaa aaaccatccc
    12841 ataaaccaag taaatagatt gccagcaatg acggctccgg cagcagcgcg ttcaggcaca
    12901 gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa aatcactttt ttgcgctcat
    12961 agcgatccgc cagcacaccg ccaaccatca ggccaacaaa catcgcgccg ccggtcagcg
    13021 tcaccgaaag ccccacctgc caggtagaat gcgtcatcat ctggatctgc accggcaccg
    13081 cgacgccgag caaacccaga gacacaattg agataaaacg agcgaggaat actgcgcgaa
    13141 acgccgggtg cgttttcaac aggctgaggt taagcagcca ggattgttta ttcattacaa
    13201 tgccttgcca tcaaatattt taaataccta ttcttaggct gcacatgcta acatatccaa
    13261 ataagatcga taacgataat taatttcatt atcatggaag ttcgtatgtc tggttctgtt
    13321 gccgtgacac gcgccattgc cgtgcccgga ttgctgttat tactgattat cgcgacggca
    13381 ttaagcctgc tcattggggc aaaatcactc cccgcgtcag tagtgctgga ggccttctca
    13441 ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg cgcgactgcc gcgcaccctt
    13501 gccggtttac ttgcaggcgg tgcgcttggg cttgctgggg cgttaatgca aaccctcacc
    13561 cgaaatccac ttgccgaccc cggcttgctt ggcgtgaacg ccggagccag ctttgccatt
    13621 gtgctgggtg cggcgctgtt tggttactca tccgcgcagg aacaactggc gatggccttc
    13681 gccggggcgc tggtggcctc attgattgtt gcctttatcg gcagtcaggg cggcgggcag
    13741 ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg cggcggtgct ggaagggctg
    13801 accagcggca tcgccctgct taatcctgac gtctacgatc aactgcgttt ctggcaagcc
    13861 ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg tgctgatccc ggttctgatc
    13921 gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca gtttgagcct cggcagtgac
    13981 accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt tgattggtct gctggcgatt
    14041 accgtgcttt gtggtagtgc gacggcggta gttggcccga ttgcctttat cggcctgatg
    14101 atgccgcaca tggcgcgctg gctggtaggt gccgatcatc gctggtcgct gcccgtcacg
    14161 ctacttgcta cccctgccct gctgctgttt gccgatatca tcgggcgggt gattgtgccc
    14221 ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg caccggtgct gatcttcctc
    14281 gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc tcgccgatta ctcatcacct
    14341 gtttgctgct gatttccgcc tgtgtggttg caggtatctg gggattacgc agcggtgccg
    14401 tcacgctgga aatctcgcag gtattcgccg cgttgatggg cgatgcgccg cgcagtatga
    14461 cgatggtggt caccgaatgg cgtttaccac gcgtgctgat ggcgctgttg attggcgcgg
    14521 cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg taacccgctc ggcagccctg
    14581 acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct ggtggcgatg gtgctgtttg
    14641 gtcaggacct gacggctatc gcgctggcag caatggtggg cggcattgtc acttctctgt
    14701 tggtctggct gctcgcctgg cgtaacggta tcgacacctt tcggttgatt attatcggca
    14761 tcggcgttcg cgccatgctg gtggccttta atacttggct gttgctgaaa gcgtctttag
    14821 aaacggcgct aacagcgggt ttgtggaatg ccggatcgct caatggcctg acgtgggcaa
    14881 aaacttcgcc ttccgcaccc atcatcatat tgatgctcat tgccgccgcc ttgctggtgc
    14941 ggcgaatgcg cttgctggag atgggcgatg ataccgcctg cgcgctgggc gtcagcgtcg
    15001 agcgttcgcg tctgttaatg atgctggttg cagtggtgct taccgctgcg gcaacggcgc
    15061 ttgccgggcc gatttccttt attgctttag tcgcgccgca cattgcccga cgcattagcg
    15121 gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg agcgctgtta ctgctggcgg
    15181 ccgatctctg cgcccaacaa ctgtttatgc cgtatcaact tccggttggc gtcgttaccg
    15241 tcagcctcgg cggtatttac cttatcgtct tgttaattca ggagtctcgc aaaaaatgac
    15301 cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg ggatatggca aatataccgt
    15361 tgcggaaaat ctgactgtag aaattcctga tggtcacttc acggcaatta tcggaccaaa
    15421 tggctgtggt aaatccacgt tactgcgtac cttaagccgc ctgatgacgc ctgctcacgg
    15481 gcatgtctgg ctggatggcg agcacattca acattacgcc agtaaagaag ttgcacgccg
    15541 gattggtctg ttggcgcaaa atgctaccac gccgggcgat atcaccgtgc aggagctggt
    15601 ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc tggcgcaaag aggatgaaga
    15661 agcggtaacg aaagcgatgc aggccacgag aataactcat ctggcagatc aaagcgtgga
    15721 taccctttct ggcggacaac gccagcgagc gtggatcgcg atggtgctag cccaggaaac
    15781 ggcaattatg ctgctcgacg aacccacgac ctggctggat atcagtcatc agattgatct
    15841 actggaactg ttaagcgaac tgaaccgcga gaaaggctat accctggcgg cggtgctgca
    15901 cgatcttaat caggcctgtc gttacgccag ccatttgatt gcattgcggg aagggaaaat
    15961 tgttgctcag ggagcgccga aggagattgt cactgctgaa ctgattgagc gcatttatgg
    16021 tctgcgatgc atgatcattg acgatccggt agccggaacg ccgcttgtgg tgccgctcgg
    16081 acgaacggca ccttcaaccg caaatagtta aactaagtgg tctgccatca tggcatcctg
    16141 ttttctggat gccatcgcat agcgcaataa cacgctacca caagccacca tcccgccgat
    16201 cagcgcggaa aggatcacaa taatcgcctt acccggaccg tcttttttca ctggcaatga
    16261 cgggcttaac tgatatttaa acggcgtaaa attcacatcg ttgacatttg cttttgttaa
    16321 ttgctcgaca agatactgcc gattacgtaa ttcaccgttc agttccgcaa cgtcagtaac
    16381 cgctttttca atttccagtt tgcgttcaat accgtctgcg ccgagagaaa tagagaaatc
    16441 ggggtcatct ttaacggcct gaccattact gtatacgggc tttttaattc ctgccgcgtt
    16501 ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca tcaagttgat ttttcgtttt
    16561 aatgcgatcc tgagccagtt tttctttttc aaactgggtt ttgatctcca gtttattacg
    16621 gacgttttct atcgactctt tcaccaccaa cgcagagata taatcgatat acccgctcaa
    16681 aacggtctgc gcctctttac tggttggcgc ggtaaaactt agcgtccagg aggtatacag
    16741 tgacggttca tcttttttct tactggcatt gtcatcaacc gctttcattt tttcgctcaa
    16801 tgcaacaata gcgcgatgca aatccagttc atcgattttc gcctctttta attggtccat
    16861 cacataaggt gatgaacgca ggtactcttc cagcaagcta acagactgaa acttcttgat
    16921 aaacaggtta aatgcaccgc cacggtcgat attaacatcg agatcaagaa cgcggagttt
    16981 ggtaaaggtt ttctccaggt cctgccactg aatggcttcg gcgggagtaa taacggctga
    17041 gctggtccat ttttgcggca ggatgaaaga gatcagtaaa cctgcgcagg caaatgcaaa
    17101 aacgaccgcc atgaccgttt ttttggcccg ccataaaact tcgattagat taagtaaatc
    17161 aatttcatta ttactgggcg acgccagagg atactcagaa aaatgagcgt cacttccctg
    17221 tttaatattc aatgatgaca tggggtataa cctgaaaatg agtccaatag gggaaaatct
    17281 ccacggcgct cactttacca gttggtgaga attatccgaa gctgaagttt gtaaattcct
    17341 tccactgaat gcaatattca ctctatttaa gagaaatatt catcaaacct ataaatcaaa
    17401 caaataaaaa acccataatt attttctgta attatgggtt tatagataat attaatttac
    17461 ctgtttagcg ttgcgcgaat aatcggccca atttttacaa acgcccctgg agagataata
    17521 tccacatgcg cacaatcctg acgatagata tccagctccg caatccacgg cgaccaggcg
    17581 cgttcaggac tcataccttc ctgaagcgtg cgttcagcaa caaacaacgt cgctttaccg
    17641 tcaaacggta cgctatgagc agtcgtcagc aggcgcacag catcagcgta gttgccttca
    17701 atggtggtaa acaactccgt tgaagtactt ccctgctgtg ccgccaggaa ggcctcgcgc
    17761 tcgcggttaa tctccgccag cacttccggg tccagaccat tagcttcttt ttcctgccag
    17821 ttttgcgttt ctggcggcca ggtatccagc aagccaagaa atgccacctg ttcgccacgg
    17881 gcacgcagtc gcgccgcaat accctgcgcc agcgtaccgc caagggaata ccccagcagg
    17941 taataagggc cgtgcggttg ttgctcaagt aacgttgcca gatgcgcttc gcagacttca
    18001 tccaggtttg tcgctgtctg catgggtcca tgagggcgcg gcgactgaat gccaataatc
    18061 gaccattgtg gatcgagata acgcgagagt acgctaaact gccaggcaaa accggacgcc
    18121 ggatggaaac aaaacagcgt cgggccatta ccttcacgca acggcagaat ggtttcgaat
    18181 cccatgcgcc gggagctgtc ctcttcacca tcaataatcg ttgccagttt ggcgacggtt
    18241 gacgcgacca tcacctgccc cggcgtcacc tgacgagcaa actgtcgact taactgcgct
    18301 gccagtttca ttgccagtag cgaatgaccg cccagcgcaa agaaatcagc atcggcatcc
    18361 tgcacgtcac aacccagcaa cgacgcgaat gccgcggcga taatcgtttc actgcccgct
    18421 ttcggtgcac gtcccgacgc ttgtgtcttc agttcaggca acggtaaggc tttgcgatcc
    18481 agcttgccgt tggcgctaag aggtaactgt ggaagttgca gcagaaccac cggtaccata
    18541 tgtggtggca atgtttcacg aagctgcgcc tgcaatgcgc tggtatccaa cggcaggccc
    18601 gattgcgaca ccagatagcc caccaactga cgcgcatcac caccagtggc tgccgcctgg
    18661 ttaatcacac aggcgtgggt aacggcttgt tcgacatccg gcagcgcctg catcacgcga
    18721 tcgatttcgc ccagttcgat acgctgcccg cgaattttta gctgatcgtc actgcgcccg
    18781 aggtactcca ccgcgccgtt atccagccag cgggcaacgt ctccggtacg gtacatccgt
    18841 tcgccaggag caaaaggatc ggcaataaag cggctggcgg tcagatcggg gcgtccgaga
    18901 tagccctgcg ccagttgaat gccggtaaga tagagatcac ccgccacacc cggcggcacc
    18961 ggatgcatca tcgcatcaag aatacgcagc cccatgttcc acaccggata accaatcggc
    19021 acactgctgc cgcgcacctg tgccagttcc tcgccaaaag ccggatacca gctgacatct
    19081 accgccgctt ccgtcgggcc atacagatta tgtaacggcg caccggttaa ctgttgccat
    19141 tcacggcata aatcagccgg taaggcttca ccactacaga aaacctgttt caacgtcgcg
    19201 caactctggc gagcggtttg cggagtcagc gaggcaacaa atgccgccag catcgacggc
    19261 acaaagtgcg tggtcgttac gccatattcg gcaaagaatt gctgcatagc gagcgggtca
    19321 cggtgcgctt ccggttcagc catcaccagt tttgcccctg cgataaacgg ccagaaaaac
    19381 tcccacaccg agacatcaaa actgcacggc gttttttggg caacgacatc ttcgcctgta
    19441 agcgggtagt gattttgcat ccataacagg cggttaacga tagccgtctg cccgaccatt
    19501 acccctttcg gcctgccggt ggagccagag gtaaagatga tataagccgt gtggtgcggt
    19561 tgtgaaagtt gcagcggcgc actgccctgg ggggtaagcg gggcgttata gcaaaggctt
    19621 gttaaattgg gaacatcgct aaagcgcggc agttgatcgt cggtggtaat taacagcgac
    19681 ggacgcgcat cttccagcat cattttcagg cgatcgtccg gatagccggt atccagcggt
    19741 agccaggccg cgccagcttc aactattgca tggagtgcca gggtcaaaaa gaccgagcgc
    19801 ggtagtgcca ccgccacgct gtcccctggt ttaacgccgc gctcacgcag cagattcgcc
    19861 agcgccacca cctgctcgcg catttcccga tagctgaaca ggtaacgcgc atctgccagc
    19921 gccggagcat ccggtgtttt tgccgcttgt tctgccacca gcgcgctcag cgtggtttct
    19981 ggaatctcaa cctgagtggc gttgatctgc gccagctgcg catactcacc tggcagcata
    20041 atatcgacat cgccgcacaa cagcgccgga tccgcggcaa actgggcaat cagcattttc
    20101 aggcgttcag catgctggat taacgttggc tcatcgtaac gctgtttatt ggcgaggatc
    20161 tcaatactca aatcaccgtg ttcatccggg aacagggcca gttcaaggtc attaaccgga
    20221 ccggttgcca gggtatgggt ttgcgcctga acaccaggaa tatccagttg gtaatcaaat
    20281 accttaatat tgagtaccgg accaaacagc ggttcatcac ctgccgctcg cccgctgtca
    20341 cggacaattt gttcggcatc gtaacgttga tggcgacgca tttttttcag ttgagccgcc
    20401 agtcgggttg ccagttccgg cagcgtttct tgcgccgcaa tgtgaatacc caacggtaaa
    20461 acgttgagca cgggtccgct agccgtcagc gccgccgagc ccagtcgacg cataaagata
    20521 aatccggcgg catagtccat gcgattgcac aatcgcccca gccacaaggc tgccagcgca
    20581 agggctaaat cggtacgctg cacacctgaa agttgcgtag ccagctggcg gaattccccg
    20641 tcggtaaatt ccagtttcag gcgcagaata tctgccgaag cgctgcgccc cggtaaaggt
    20701 gccggagaaa gtgacgcggg cggcggcagt tgacgacgct gttctgccca gaatgccgca
    20761 tcacgctgcc aggcttcgct ttcgcggtat tgctggtact cttccactac atcagcgaaa
    20821 ggcgtaaacg gcgaagcagg cgttggttcg ccacgcagcc atgtgcagta aatattggcg
    20881 atctggcgag taatggccgg gaaactgaag ccatcgacca gcaaatggtg ataacgctga
    20941 taccagtacc agcggttatc cgcaacatgt atcagctgat gaaagaccag tggtttaccg
    21001 ctatcgacgc gcaaatcttg ttgcaaatcc gccagcatta atgcctgcgc agtaccgtgc
    21061 ggatcaatat tggttcgcag gtcgataatt tctggctgtt cgaatatcag cgcatcatcg
    21121 acccactgcc agacttcgcc gttatcttcc gtaaaacgca tccgcaacgt atctgcttgc
    21181 gctagtccgg caaccaccgc gcgggccagt aatggcgcat caacatctcc agttaactca
    21241 acgtaatgcg ccacgctcca ggcggagggt aaatccgaca gtttttctgc catccagatg
    21301 ccgggctgtg cggcgaccaa aggtaaatgc tggctcattg tgcctcctgc aactgggtga
    21361 aattggccgg tgtcagagta cgccagtgga cttccagcca ctgctggcag gacgcctgtg
    21421 actgcggctg acacacaatg tcccagcctg ccgataagag gcattgttgc ggccacagac
    21481 tgaattgccc ctgcgcattg cgcaaaatgt aaaacgctcc ctgcggatca tcgaagggat
    21541 tactgaatgc catactcaac tcctgtcatg gaaaagtggt tgccagaggt cgattaaccc
    21601 ctgcatcaag ccaccgcgcc aacaaagcgc atcatgtccg ccgtcaacct gacgccagaa
    21661 aatggattct tttatagggt gcaattgcgc atacagcgcc tgattggccc gcatgatcat
    21721 cggctcgcga atacccgctt ccagcacaat gcgcagacct tcggcgctaa cttcaccagc
    21781 ttttagcttt tcaagtaaca cgccctcttg ctgcccgtcc cgatgcggcc accagtacga
    21841 tcctgactgg cttaatacac agccaaagcg ttcaggccag tgcagtccgg catacagcgc
    21901 ggacagccca ccaaaactct gcccggcgac cacggtgcga tcggcgcgat cgctaaaagg
    21961 ggcaatagct ttcaccaggg gtaataactc ttgctgtact gccagccaga aatccgcatt
    22021 acacggcagt tcgtgggcgc ggtgcgtggt gtcgatagcg tcgatcaaca catacacggc
    22081 gggaggaagt tgctgacgat gggtcagcga agtcagcaca ggccagacgg gcatactttg
    22141 cgcccaaaat tcgccatcga gcaaaactgc cagcggacgt tcttctgctg ttacatcgcc
    22201 ggtggtaaaa atccatacac gacgtgaatt tttcaaccgt tcacttttcc agataatttc
    22261 tttggctgct atttctggca cttgcggaca atcccatccc ggttgcagag gcgcttgcgg
    22321 catttcgagt gcagaaacag cgtgccctcg cccgcctttc cagctttgtg gattcagcgg
    22381 atcggctatt gcctggggta atagttttcg ccagccttca cgtaattcga ggcgatcggg
    22441 ggatggtgca gaaaaaatgt catcgcgttc ggtgggaata aagcagtagc tgccgcgcca
    22501 gttggcattg agttgtgtcg tccactgcca gacatcagta cctgcaattc gctgcatcga
    22561 ctggggctgg ctgttctgat gatgatcggt cacaccagtg atgtagatcc atacgcgctt
    22621 tatcgtcgag tattcttcag aaccttgggg atcacgccac cagaaagtga cctcaaacat
    22681 ttcgtcattc agacgctgcc attccgggcc atgtttcgac tgccaccagc tctcacttcc
    22741 tacttttaac gccgtcacac cataacccca tgtttactgt gcaatttttc attgattgca
    22801 gaaatatatt gataatatta ttgataacca tttgcatttg caatagcgta atggcgcgcc
    22861 gtgggaagcg cggacattta ttaaccaact gcactgcgtg tctttcagga tcaaaggttt
    22921 tcgcggtagc gggatgcgtc gtgttgagga cgaccatgcc cgacagttgc aattcgtggc
    22981 aaaaatgcag gaataaaaca atgaacaaga agattcattc cctggccttg ttggtcaatc
    23041 tggggattta tggggtagcg caggcacaag agccgaccga tactcctgtt tcacatgacg
    23101 ataccattgt cgttaccgcc gccgagcaga acttacaggc gcctggcgtt tcgaccatca
    23161 ccgcagatga aatccgcaaa aaccccgttg cccgcgatgt gtcggagatc atccgtacca
    23221 tgccaggcgt taacctgacc ggtaactcca ccagtggtca gcgtgggaat aaccgacaga
    23281 ttgatattcg cggtatgggt ccggaaaaca cgctgatttt gattgacggc aagccggtaa
    23341 gcagccgtaa ctcggtgcgt cagggctggc gtggcgagcg cgatacccgt ggtgatacct
    23401 cctgggtgcc acctgaaatg attgaacgta ttgaagttct gcgtggtccg gcagctgcgc
    23461 gttatggcaa cggcgcggcg ggcggcgtgg ttaacatcat taccaaaaaa ggcagcggtg
    23521 agtggcacgg ctcctgggac gcttatttca atgcaccaga acataaagag gaaggtgcca
    23581 ctaaacgcac caacttcagc ctgaccggtc cgctgggcga cgaattcagc ttccgcttgt
    23641 atggcaacct cgacaaaacc caggctgacg cgtgggatat caaccagggc catcagtccg
    23701 cgcgtgccgg aacgtatgcc acgacgttac cagccgggcg cgaaggggtg atcaacaaag
    23761 atattaatgg cgtggtgcgc tgggatttcg cgccattgca atcgctggaa ctggaagcag
    23821 gttacagccg ccagggcaac ctgtatgcgg gcgacaccca gaacaccaac tccgattcct
    23881 atacccgctc gaaatatggc gatgaaacca accgtctgta tcgccagaac tactcgctga
    23941 cctggaacgg cggctgggat aacggagtga ccaccagcaa ctgggtgcag tatgaacaca
    24001 cccgtaactc gcgtattccg gaaggtctgg caggcggtac cgaagggaaa tttaacgaaa
    24061 aagcgacaca ggatttcgtc gatatcgatc ttgatgacgt gatgctgcac agcgaagtta
    24121 acctgccgat tgatttcctc gttaaccaaa cgctgacgct gggtacggag tggaatcagc
    24181 aacggatgaa ggacttgagt tccaataccc aggcgctgac cggggcgaac accggcggcg
    24241 ctattgatgg tgtgagtgcc accgaccgta gcccgtattc aaaagcagaa attttctcgc
    24301 tgtttgccga aaacaacatg gagctgactg acagcaccat cgtaacgccg gggctgcgtt
    24361 tcgatcatca cagtattgtc ggcaataact ggagcccggc gctgaacata tcgcaaggtt
    24421 taggcgatga cttcacgctc aaaatgggca ttgcccgtgc ttataaagca ccgagcctgt
    24481 accagactaa cccgaactac attctctaca gtaaaggtca gggctgctat gccagcgcgg
    24541 gcggctgcta tcagcaaggt aacgatgacc tgaaagcaga aaccagcatc aacaaagaga
    24601 ttggtctgga gttcaaacgc gacggctggc tggcgggcgt cacctggttc cgtaacgatt
    24661 atcgcaataa gattgaagcg ggctatgtgg ctgtagggca aaacgcagtc ggcaccgatc
    24721 tctatcagtg ggataacgtg ccgaaagcag tggttgaagg tctggaagga tcgttaaacg
    24781 taccagttag cgaaatggtg atgtggacca ataacatcac ttatatgctg aagagtgaaa
    24841 acaaaaccac gggcgaccgt ttgtcgatca tcccggagta cacgttgaac tcaacgctga
    24901 gctggcaggc gcgggaagat ttgtcgatgc aaacgacctt cacctggtac ggtaagcagc
    24961 agccgaaaaa atacaactac aaaggtcagc cagcggttgg cccggaaacc aaagaaatca
    25021 gtccttacag cattgtgggt ctgagcgcga cctgggatgt gacgaagaat gtcagtctga
    25081 ccggcggcgt ggacaacctg ttcgacaaac gtttgtggcg tgcgggtaat gcccagacca
    25141 cgggcgatct ggcaggggcc aactatatcg cgggtgccgg ggcgtatacc tataacgagc
    25201 cgggacgtac gtggtacatg agcgtaaaca ctcacttctg atgctaacgt cagattgttg
    25261 acaaagtgcg cgtcgttcat gccggatgcg gcgtgaacgc cttatccggc ctacaaaaat
    25321 cttgccaatt caatatattg caggaccatg taggcctgat aagcgtagcg catcaggcag
    25381 ttttgcgttt gtcatcagtc tcgaatatgg tcgatatgaa aactacgcat acctccctcc
    25441 cctttgccgg acatacgctg cattttgttg agttcgatcc ggcgaatttt tgtgagcagg
    25501 atttactctg gctgccgcac tacgcacaat tgcaacacgc tggacgtaaa cgtaaaacag
    25561 agcatttagc cggacggatc gctgctgttt atgccttacg ggaatatggc tataaatgtg
    25621 tgcccgcaat cggcgagcta cgccaacctg tctggcctgc ggaggtatac ggcagtatta
    25681 gccactgtgg gactacggca ttagccgtgg tatctcgtca accgattggc attgatatag
    25741 aagaaatttt ttctgtacaa accgcaagag aattgacaga caatattatt acaccagcag
    25801 aacacgagcg actcgcggac tgtggtttag ccttttctct ggcgctgaca ctggcatttt
    25861 ccgccaaaga gagcgcattt aaggcatcgg agagacaaac tgaagcaggt tttctggatt
    25921 atcagataat tagctggaat aaacagcagg tcatcattca tcgtgagaat gagatgtttg
    25981 ctgtgcactg gcagataaaa gaaaaaatag tcataactct gtgccaacac gattaattga
    26041 caacatctgg tacgattcgc ccgcagccat cactgactgc gggcgaaagt gtaaagcagg
    26101 tgccttacca ctctgacctg acaaccggat atgcagggat tgctccccgc acaacggcta
    26161 cttcttcggt tcgtaagcga gaacagcctt aaactcaata ttacgttcct ttacggtgaa
    26221 ctcacacagc gagtctccga ccagaagcgt aaatcccagc accgtcaaac acagcactat
    26281 gactgccaca agggcatatt tcgtcagcat gtttatattg cctccatgcg aagaagggac
    26341 taccatccga gttgttgagg ttcggagtgg cagccccagg attgatagaa atatctcctg
    26401 gggcttcatc tttccagacc tcagagtaaa cctgaaaccg gaaagcttca ggcacccgcc
    26461 gttatcttac ccttcctcag caaaatatct gtttatttat acagtaagtc ctgtttcgga
    26521 cttctgttgg caacactcgt tattgctcgt aaaacgaaat cgcccgcagc catcactgac
    26581 cgcgggcgaa agtgtaaagc aggtgcccta ccatcctgac ctgacaaccg gatatgcggg
    26641 gattactccc cgcacaacgg ctacttcttc ggttcgtaag cgagaacagc cttaaactca
    26701 atattacgtt ccttcaccgt aaactcacac agcgagtctc cgaccagaag cgtaaatccc
    26761 agcaccgtca aacacagcac tatgactgcc gcaagggcat atttcgtcag catgtttata
    26821 ttgcctccat gcgaagaagg gactaccatc caaattgttc gggtttggag tggcagcccc
    26881 agg
//
LOCUS       DAJVRF010000045        26439 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJVRF010000045 DAJVRF010000000
VERSION     DAJVRF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26439
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.045"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            156..239
                     /locus_tag="OQN13_001727"
     CDS             156..239
                     /locus_tag="OQN13_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1615105.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            339..1160
                     /locus_tag="OQN13_001728"
     CDS             339..1160
                     /locus_tag="OQN13_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCS1615106.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1199..1528)
                     /gene="mdtI"
                     /locus_tag="OQN13_001729"
     CDS             complement(1199..1528)
                     /gene="mdtI"
                     /locus_tag="OQN13_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCS1615107.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1515..1880)
                     /gene="mdtJ"
                     /locus_tag="OQN13_001730"
     CDS             complement(1515..1880)
                     /gene="mdtJ"
                     /locus_tag="OQN13_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCS1615108.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2292..3326
                     /gene="tqsA"
                     /locus_tag="OQN13_001731"
     CDS             2292..3326
                     /gene="tqsA"
                     /locus_tag="OQN13_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCS1615109.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLISVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3351..4739)
                     /gene="pntB"
                     /locus_tag="OQN13_001732"
     CDS             complement(3351..4739)
                     /gene="pntB"
                     /locus_tag="OQN13_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCS1615110.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(4750..6282)
                     /gene="pntA"
                     /locus_tag="OQN13_001733"
     CDS             complement(4750..6282)
                     /gene="pntA"
                     /locus_tag="OQN13_001733"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCS1615111.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            6806..7750
                     /locus_tag="OQN13_001734"
     CDS             6806..7750
                     /locus_tag="OQN13_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1615112.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            7936..9318
                     /locus_tag="OQN13_001735"
     CDS             7936..9318
                     /locus_tag="OQN13_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1615113.1"
                     /translation="MKKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9355..10077
                     /gene="folM"
                     /locus_tag="OQN13_001736"
     CDS             9355..10077
                     /gene="folM"
                     /locus_tag="OQN13_001736"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCS1615114.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10074..10409)
                     /locus_tag="OQN13_001737"
     CDS             complement(10074..10409)
                     /locus_tag="OQN13_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCS1615115.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10538..11257
                     /gene="rstA"
                     /locus_tag="OQN13_001738"
     CDS             10538..11257
                     /gene="rstA"
                     /locus_tag="OQN13_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCS1615116.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11261..12562
                     /gene="rstB"
                     /locus_tag="OQN13_001739"
     CDS             11261..12562
                     /gene="rstB"
                     /locus_tag="OQN13_001739"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCS1615117.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            12638..13567
                     /gene="tus"
                     /locus_tag="OQN13_001740"
     CDS             12638..13567
                     /gene="tus"
                     /locus_tag="OQN13_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCS1615118.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13564..14967)
                     /gene="fumC"
                     /locus_tag="OQN13_001741"
     CDS             complement(13564..14967)
                     /gene="fumC"
                     /locus_tag="OQN13_001741"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCS1615119.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(15110..16756)
                     /gene="fumB"
                     /locus_tag="OQN13_001742"
     CDS             complement(15110..16756)
                     /gene="fumB"
                     /locus_tag="OQN13_001742"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS1615120.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            16955..18130
                     /gene="manA"
                     /locus_tag="OQN13_001743"
     CDS             16955..18130
                     /gene="manA"
                     /locus_tag="OQN13_001743"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCS1615121.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            18231..19739
                     /locus_tag="OQN13_001744"
     CDS             18231..19739
                     /locus_tag="OQN13_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCS1615122.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(19920..21230)
                     /gene="uidC"
                     /locus_tag="OQN13_001745"
     CDS             complement(19920..21230)
                     /gene="uidC"
                     /locus_tag="OQN13_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCS1615123.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(21269..22642)
                     /gene="uidB"
                     /locus_tag="OQN13_001746"
     CDS             complement(21269..22642)
                     /gene="uidB"
                     /locus_tag="OQN13_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCS1615124.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGFCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(22639..24450)
                     /gene="uidA"
                     /locus_tag="OQN13_001747"
     CDS             complement(22639..24450)
                     /gene="uidA"
                     /locus_tag="OQN13_001747"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCS1615125.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(24839..25429)
                     /gene="uidR"
                     /locus_tag="OQN13_001748"
     CDS             complement(24839..25429)
                     /gene="uidR"
                     /locus_tag="OQN13_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCS1615126.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(25650..26417)
                     /gene="hdhA"
                     /locus_tag="OQN13_001749"
     CDS             complement(25650..26417)
                     /gene="hdhA"
                     /locus_tag="OQN13_001749"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCS1615127.1"
                     /translation="MRHAPCAMRHAPCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         6608 a         6853 c         6352 g         6626 t
ORIGIN      
        1 aaacacgccg ggaaacacag ccatcagcaa ccggcaaaac ctgctgcaca acccgcagcg
       61 taagttttca acagtaatgc tggcgcgccc cctcgcgcct gaaaattacg gtgctaagcg
      121 ggtaacgttt agcaccgcct ttagcggagg gtagtatggt gggccgttat cgctttgagt
      181 tcattcttat catccttatt ttatgcgcac tgattgccgc ccgtttttat ctttcctgac
      241 tgtagttatc tgattttact cccactttca tcccgtcccg tctatagtat ttatgagggt
      301 ttgcttttaa taatcataat tacccaccag agtgtgatat gcgtacaacc attgctgtag
      361 tgttgggtgc aattagtttg acgtctgctt ttgtgtttgc agataaacca gacgttgcca
      421 aatcggcaaa cgatgaggtc agcaccctgt tttttggtca tgatgatcgt gtgccagtga
      481 atgacacgac ccaatcaccg tgggatgcgg ttgggcaact ggaaacggcc agcggcaatt
      541 tatgtacggc gacgctgatt gcacccaatc tggcattaac ggcaggacac tgtttattga
      601 cacctccaaa gggtaaagcc gataaagcag tggcgctgcg ttttgtgtca aataaaggtc
      661 tttggcgcta tgagatccac gacatagaag gccgcgttga tccgacactg ggaaagcggt
      721 taaaagcaga tggggatggt tggattgtac ctcccgcagc cgcgccgtgg gacttcggat
      781 tgattgtgct acgtaatccc ccttctggca ttacaccgtt gccgttattt gagggagata
      841 aagccgcgct tactgccgca ttaaaatcgg ctggccgtaa agtaactcag gcgggatacc
      901 ctgaagatca tctcgatacg ttgtacagtc atcaaaactg tgaagtgact ggctgggcgc
      961 aaacgtcggt gatgtcacat cagtgcgata ccttgccggg tgacagcggt tcgcctctga
     1021 tgttgcatac caatgacggc tggcaattaa ttggggtgca aagttcggct cctgccgcga
     1081 aagatcgctg gcgcgccgat aaccgggcca tttctgttac cggttttcgc gacaagctgg
     1141 atcaactgtc gcaaaaataa tgttcaaacg ctgcccgact gtgcgggcag cgtgttcatc
     1201 aggcaagttt caccatgatc attccagcca acagcaagac caggccaatc cagcctttac
     1261 gatttaaccg ttgaccaaac aagatccaac ctgcggctaa cgtggcggca ataccaaacc
     1321 cgccccacaa tgcataagcg acagacaagt cgatcccttt aacggcctga gaaagcgcac
     1381 taaaggcagc cagcaccgcc gccagggaga gcaagccaaa tattttgcga cgaaagccgt
     1441 cagaaaattt caaaaagacg ttagcaacga tttccagcac gattgccaat gccagccagg
     1501 cggcgtgaac ccattcaaac tgcgccatgg ttcacctcca gttcaggttt acgcgcttta
     1561 cgggtacctg attttatcaa cacaatcccg gcgaccaggg tggttaaccc ggcaattttc
     1621 atcagcgata aactttcgtc gaataacaaa acgctaaaca aggtaataaa taaaataccg
     1681 ataccttccc acagcgcata agctacgcct aaggcgatct ttttaacggc gaaagagaga
     1741 aatatatacg acagagaaat catcaccagc attaaaataa agccgccatt tccctcactg
     1801 acgctcgccc atttcattga cagcgtaccg gtaatttctg tagcaatagc caggcctaat
     1861 aaaatccaat aaatatacat tgtccttctc ctgcaagaga attattttaa ttttcgctta
     1921 attcagcgaa accaaagtaa agtggtcaag ccccgcgcat agggcaagct taggcagaag
     1981 aaaggactaa agcgcgttgc gccagtgctg ctcacctacg agcaagatag tggatgaggt
     2041 acgaagatgg gtaaatgttg aaaataacgt cctgaacaaa ttgtccataa tattacaatt
     2101 atccgcagtg ttgcttctcg tcatcgcgga tgataattgt cctcggtagt tgaacacgcc
     2161 tgatttgtat catagcttaa gaattaactc aaaatatttt cacttcttta cctgagcggt
     2221 ttgattttcg ttatgatgac ggagcgaaaa agacattatt attagcaaag gaagaaaaaa
     2281 cggggacaag catggcaaag ccgatcatca cgctcaatgg cctaaaaatc gtcattatgt
     2341 tgggaatgct ggtcattatt ctctgcggta tccgttttgc cgccgagatc atcgtgccgt
     2401 ttattctcgc attatttatt gctgttattc ttaacccgct ggtgcaacac atggtccgct
     2461 ggcgagtgcc gcgtgtactg gcggtgtcga ttttgatgac catcatcgtg atggcgatgg
     2521 tgttgctatt agcttatctg ggttccgcgc tcaacgagtt gacgcggacg ttaccgcaat
     2581 atcgcaactc tattatgacg ccgctgcaag ctcttgagcc gttgttgcaa cgcgtaggga
     2641 ttgacgtctc agttgaccag ctggcgcatt acattgatcc gaacgcggcg atgacgttgc
     2701 tcaccaactt attgacgcag ttatctaatg ccatgtcatc aatattttta ttgctgctga
     2761 cggtgctgtt tatgctgctc gaagtgccac aattgcccgg aaaatttcag caaatgatgg
     2821 cgcgtccggt tgaagggatg gcggcgattc aacgtgcaat tgacagtgtt tctcattatc
     2881 tggtgctgaa aacagccatc agcatcatca ccggcctggt cgcctgggcg atgctcgccg
     2941 cactcgatgt tcgcttcgct tttgtctggg gattgctggc ctttgcgctt aattacatcc
     3001 ctaatattgg ttcagttctc gcggcaatcc cccctatcgc tcaggtactg gtgtttaatg
     3061 gcttctacga agcgttgctg gtgctggcgg gatatctgct gattaatctg gtcttcggca
     3121 atattctgga gccgcgcatc atgggacgtg ggctggggct ttccacattg gtggtatttt
     3181 tgtcgttgat tttttgggga tggttgttag gaccggtggg tatgctgatt tccgtgccgt
     3241 taacaattat tgtcaaaatt gcgcttgaac aaacagcggg aggtcaaagc atcgccgttc
     3301 tgttaagcga tctcaataaa gagtgacggc ctccgcagag gccgtcaggg ttacagagct
     3361 ttcaggattg catccacgct agctttggcg tcaccaaaca gcatgtgggt gttttccttg
     3421 aagaacagcg ggttttgcac accagcatag ccagtgttca tcgaacgttt aaagacaatc
     3481 acgttctgcg ctttccacac ttccagcaca ggcataccag caataggact cttcggatca
     3541 tcctgcgccg ccgggttaac cgtatcgtta gcaccaatca ccagtacggt atcggtatca
     3601 gcaaagtcat cgttgatctc gtccatttcc agcacgatgt catacggtac ttttgcttca
     3661 gccagcaata cgttcatatg tccaggcaaa cgccccgcaa ccgggtggat accgaaacgc
     3721 acgttgatac cacgagcgcg cagtttctcg gtaatttcag cgacaggata ttgcgcctgc
     3781 gcgactgcca tgccgtaccc cggagtaatg atcactgaat gggagttttt cagcagttcc
     3841 gctgtctctt ctgcggtgat ttcgcggtgc tcgcccactt cctgatcatc gccagtagaa
     3901 gagccgtcgg tgccgaaacc acccgcaata acgctgataa aggaacggtt catcgcctta
     3961 cacataatgt aagaaaggat agcccccgaa gaaccgacca gcgcaccggt cacaatcagc
     4021 aggtcgttgc tgagcataaa gcccgcagcc gcagccgccc agccggagta cgagttcagc
     4081 atcgacacca ccactggcat atctgcacca ccgatggagg cgactaaatg ccagccgaat
     4141 accagcgcaa tggcggtcat tattagcaat gccagcactt gcaggccgac gctgtccgtg
     4201 cgaacaaata caatcagcag caggaaggaa acgaccagag ccgccaggtt cattttgtga
     4261 cggtttggca gcatcaacgg tttagacgaa atcttgccac acagtttgcc gaacgccacc
     4321 accgaacccg tgaacgttac cgccccgatg aagataccga ggaacacttc cgtcaggtga
     4381 atattgacca gaatcggtgc cattcccgcg tcatgatgca gatagctgtt aaagccaacc
     4441 agcactgccg ccagacccac gaagctgtgc aggatcgcca ccagttctgg catttcggtc
     4501 atttcaactt tcttcgccag acggatacca attgccccac caatgaccat cgccagcaag
     4561 atccagccaa cattacccgt atccggtcca aaaatggttg cgattaacgc aatcgccatc
     4621 ccggcgatac cgaagttgtt accctggcga gacgtttcat gtttcgaaag accggccaga
     4681 ctgaagataa acaggatcgc ggcaacaatg tatgcagctg taactaatcc tccagacata
     4741 tgttacccct taatttttgc ggaacatttt cagcatgcgc tgagtcacgg tgaagccacc
     4801 gaaaatatta atgctggcta taagcaccgc gataaaacta aggaagctaa cccagccgcc
     4861 ctggccaatc tgcaacagtg ctccgacaac aataatccct gaaatcgcgt tggtgaccga
     4921 catcaacggt gtatgcagcg cgtgcgatac attccacacc acgtaataac cgacaacgca
     4981 ggccagcgcg aaaacggtga agtgcccaag aaactctttc ggcgcaacgc ttgccagcca
     5041 accgaaaagg atgattgcca gcgccatcaa cgcgtattta cgccacggtg agcaggcaca
     5101 tttttcctca gttttcactt ccggtgccgc tttttgtgcc gcctgcggct gagctgatac
     5161 ctgaatcggc ggtgccggcc aggtaatttc gcccgcacgg atcacggtca cgccgcgaat
     5221 caccacatca tcaaaatcaa cagtgatatt gccgtctttc tctttgcaca acagtttcag
     5281 cagattaacg aggttagtac cgtaaagctg cgaggattgc gtcggcagac ggcccggaag
     5341 atcggtataa ccaatcactt tgacaccatt ttccgtagtg aagatttcac ccggcacggt
     5401 gtattcacag ttgccaccgt tttgggctgc caggtcgaca atcacactgc ccgccttcat
     5461 ggagtcaacc atttcacggg taattagctt cggcgctggt ttgcctggaa taagcgcggt
     5521 ggtgacaatg atatcgacct cttttgcctg ggcggcaaag agttccattt ccgctttaat
     5581 gaacgcgtcc gacatcactt tggcatagcc atcgccgctg cccgcttcct ctttaaaatc
     5641 cagctcgagg aattccgcgc ccatactttg aacttgttct ttcacttccg gacgggtgtc
     5701 gaatgcacgc acaatcgcgc cgagactgtt tgctgcgcca atggcggcca gacctgcaac
     5761 acccgcacca atcaccatca cttttgccgg tggcactttc ccggccgcag taatttgccc
     5821 ggtaaagaag cgcccaaatt catgtgccgc ttcaacaatg gcgcgataac cggcgatgtt
     5881 cgccatcgag cttagtgcgt ccagcgattg tgcgcgtgag atacgcggca cagaatccat
     5941 cgccatcacg gtcacgttac gttccgcaag tttttgcatt aattccggat tctgcgcagg
     6001 ccagataaaa ctcaccagcg ttgtcccagg attcagtaac gcaatttcat catctaacgg
     6061 cgcattgacc ttcagaatga tctctgactg ccagacgcta ttcccttcta caatttcagc
     6121 gcccgcttgc acaaacgctt tatcgtcaaa acttgccagt tgacccgcgc cgctctctac
     6181 cgcgacggta aaacccagtt tcagcagctg ttccactgtt tttggcgttg ctgcaacacg
     6241 ggtttcattg gttaaccgtt ctcttggtat gccaattcgc atgatattcc cttccatcgg
     6301 ttttattgat gatggtttgc ctgtgtcagg agccacacaa gccgctcatg tacgagctaa
     6361 atgttactcc gttaaaataa attagtaaca aacgccctat aacgtactga aaattatgcc
     6421 tgtgatctag cgccaaaaat cagtatttcg gcgtgaactc gcaaaatatt aacgattcag
     6481 ccgtgatagt gggataaaca ccttagaacg ccggataaag actgataatt gtcttcgacg
     6541 gccgggtaaa acgagacatc gccccggcac gattcactac ttaacattaa attaacttat
     6601 acaattcagt tgcttcagta gtaatgatgc tgatacggct gttttttaag catagacggt
     6661 catttgagca ggattaaaat tggcttaagg aatgtgatat gaaaaatgac gcagacagtt
     6721 acaccgttta aatgcaataa tcagccacgt ttctcgttaa taacaatacc agtacctggt
     6781 ttgcgcaagg cgaaggatta tttttatgaa gcttaagaac accctcctgg cgtcggcact
     6841 gctttctgct acggcattct ccgttaacgc agcaacagaa ctgacaccgg agcaagcggc
     6901 agcggttaaa ccttttgacc gtgtagtggt taccggtcgt tttaatgcta ttggcgaagc
     6961 ggtgaaagcc gtttctcgtc gcgcagataa agaaggtgcc gcctcttttt atgttgtcga
     7021 cacttctgat tttggtaaca gcggtaactg gcgtgtggtc gctgacctct ataaagccga
     7081 tgctgaaaaa gcagaagaaa caagtaatcg cgtaattaac ggtgttgtcg aactgccgaa
     7141 agatcaggct gttctgattg aaccgtttga cacggtcacc gtccagggct tctatcgtag
     7201 ccagccagaa gtcaatgatg ccatcaccaa agcggcaaaa gcgaaaggtg cctactcttt
     7261 ctacatcgtt cgtcaaatcg atgccaacca aggcggcaac cagcgtatta ctgcattcat
     7321 ctataaaaaa gatgctaaga aacgtatcgt ccagagcccg gatgtgatcc cggcagattc
     7381 cgaagcagga cgtgcagctc tggctgccgg tggcgaagcc gcgaagaaag ttgagatcct
     7441 aggtgttgcg actaccgcat caccaagttc tgaagtcggt cgtttctttg aaacccagtc
     7501 atcaaaaggc gggcgttaca ccgtcatgct cccggatggc actaaagtcg aagaactgaa
     7561 caaagcgacc gcagcgatga tggtcccgtt cgacagcatc aaattctctg gcaactacgg
     7621 caacatgacc gaagtctcct atcaggttgc gaaacgtgcc gcgaagaaag gtgctaagta
     7681 ttaccacatc acccgccagt ggcaggaacg tggtaataac ctgaccgtca gcgcagatct
     7741 gtataaataa ctattcagtt actcctataa ggcggcttga tgccgccttt cgcattttta
     7801 gtcacaaatt tccccgcgac cattgcatgc agacacatct cttcgtaaaa tcccgcgctt
     7861 cagtggcaaa ctccattttt atgcgttttg ccctaatatt tattcttctt atcacgtttt
     7921 aatcactgga tatcgatgaa aaagaaactg ggactgagcg cactcaccgc gctggtatta
     7981 agctcaatgc tgggcgcggg tgttttcagt ctgccgcaaa atatggcggc agttgccagc
     8041 ccggcagcac tgctcatcgg ctggggtatt actggcgctg gcattttatt gctggccttt
     8101 gccatgctga tcctcacgcg cattcgtccc gaacttgacg gcggtatctt tacctatgcc
     8161 cgcgaagggt ttggcgagct aatcggcttt tgttccgcat ggggatactg gctgtgcgca
     8221 gtcatcgcca acgtctccta tctggtgatc gttttttccg cgttaagctt ttttacggac
     8281 acgccggaat tgcgcctgtt cggcgatggt aatacctggc aatcgatcgt cggtgcatcg
     8341 gctttattgt ggattgttca ctttttgatc ctgcgcgggg tgcaaaccgc tgccagcatt
     8401 aacctggtgg cgacactggc aaaattgttg ccgctgggtc tgtttgttgt gctggcgatg
     8461 atgatgttca aactggatac cttcaagctc gacttcaccg gacttgcact tggcgtaccc
     8521 gtttgggaac aggtgaaaaa caccatgctg atcaccctgt gggttttcat tggtgtggaa
     8581 ggtgccgtgg ttgtttctgc gcgtgcgcgt aataaacgtg atgttggcaa agcgacactg
     8641 ctggcggttc tctccgctct gggcgtttac ttgctggtaa cgctgctttc gctgggtgtg
     8701 gtggcacgtc ctgaactggc tgaaattcgt aacccgtcaa tggccggtct gatggtggaa
     8761 atgatgggac catggggcga aatcatcatc gctgccggtt tgatcgtttc cgtttgcggc
     8821 gcgtacttaa gctggaccat catggcggca gaagttccgt tcctggctgc cactcataaa
     8881 gcattcccac gcatttttgc gcgccagaac gcacaagctg cgccatccgc ctcactgtgg
     8941 ttgaccaaca tctgcgtgca aatttgtctg gtactcatct ggctgaccgg ttccgattac
     9001 aacacgttgc tgaccatcgc ctcagaaatg attctggtgc cctatttcct ggtcggcgcg
     9061 ttcttgctga aaatcgcaac ccgtccactg cataaagcgg taggtgtcgg tgcctgcatt
     9121 tatggcttat ggttattgta cgcctctggt ccaatgcact tgctgttatc cgttgttctg
     9181 tatgcgccgg ggctgctggt tttcctgtat gcgcgcaaaa cgcatacaca tgataatgta
     9241 ctgaaccgtc aggaaatggt gctgattggc atgctgttaa ttgcctcagt tccggcgacc
     9301 tggatgctgg tgggataacg ttcatcccat cgttggatag caaaggagat aacgatgggt
     9361 aaagcccagc ccttgccaat attaattact ggcggaggtc gtcgcatcgg cctcgccctc
     9421 gcctggcatt tcattaatca aaagcaaccg gtgattgtca gctatcggac gcactatcca
     9481 gccattgatg gactgattaa ggcaggtgcg cagtgtattc aggctgattt ttcgaccaac
     9541 gacggtgtga tggcgtttgc cgatgaagta ctaaaaagcc cccatggtct gcgtgctatt
     9601 ttgcataacg ccagtgcgtg gatggcggaa aaaccgggtg cgccactggc cgacgtactg
     9661 gcttgcatga tgcagatcca cgttaatacc ccatacctgc tcaaccatgc gctggaaaga
     9721 ttactgcgtg ggcacggaca cgccgccagc gatatcattc actttaccga ttatgtggtg
     9781 gagcgcggta gcgacaagca tattgcgtat gctgcaagca aagcggcact ggataatatg
     9841 acccgctcgt ttgcccgcaa gctggcaccg gaagtgaaag tgaactctat tgctccatcg
     9901 ctgatcctgt ttaatgaaca tgatgatgcc gaatatcgac aacaggcgct gaataaatca
     9961 ctgatgaaaa ccgcgcctgg cgagaaagaa gtgatcgacc tggtcgatta cttacttacc
    10021 agttgctttg tcaccggacg cagtttccca cttgatggcg gtcgtcatct gcgttaatgc
    10081 agctttatcc agcatataat caacacccat gcgcttatcc cccaacacga aacagaacca
    10141 acaaaagcgg cgggcaagcg catcattccg gtgaaatacc acagcgacac caggtaaaca
    10201 aaatagggaa taatcgacca catactaaaa atgatggttg cgcgtaaggc ttcaatgccg
    10261 cgttcgctgg caacaatata atgcgcgata agcgcaaagg tcgggaaaag tggaatcagc
    10321 ccggcgatat aataattttt cgtttttgct aaaacaccaa tcaacagcac taccagcgca
    10381 ccgagcgcgg ctttgattac cagccccatt tttttacctt aacacttcca taacaagtca
    10441 tcagtagaat acctgatgaa aacttgttta gaaacgattg atagtaagta aaaacagcgc
    10501 ggtgtattgt gacgttttta tatctaccgt gaatgttatg aacactatcg tatttgtgga
    10561 agatgatgcg gaagtcggtt cactgattgc cgcgtacctg gcaaaacatg atatgcaggt
    10621 taccgtagag ccgcgcggcg accaggccga agaaaccatt ttgcgagaaa atccggattt
    10681 ggtgttactc gacatcatgc taccaggcaa ggacggcatg accatttgtc gtgatttacg
    10741 cgcaaagtgg tctggaccga ttgttcttct aacctctctc gatagcgata tgaaccacat
    10801 cctggcactg gaaatgggtg cctgcgacta tattctcaaa acgacgcccc ctgctgtttt
    10861 gctagcgcgt ttacgtttgc atttgcgtca gaatgagcaa gccacactga ccaaaggtct
    10921 tcaggaaacg tctctgactc cctacaaagc cctgcatttc ggcacgttga ccatcgatcc
    10981 catcaaccgc gtagtcaccc tggctaacac tgaaatctcg ctctcgacag ctgatttcga
    11041 attattgtgg gaattagcta cccatgccgg gcaaatcatg gaccgcgatg cattgctgaa
    11101 aaatttacgc ggcgtcagtt atgacggact ggatcgtagc gtggacgtgg ctatttcgcg
    11161 gttaagaaaa aaactgctcg ataacgccgc agaaccttat cgcattaaaa ctgtgcgtaa
    11221 caaaggctat ctttttgcgc ctcatgcatg ggaataagcg atgaaaaaac tgtttatcca
    11281 gttttacctg ttattgtttg tctgcttcct tgtgatgtct ctgctggttg ggctggtgta
    11341 caaatttacc gccgaacgcg cgggcaaaca gtcgctggat gatttgatga acagttcact
    11401 gtatctgatg cgcagcgaat tgcgtgagat ccccccacac gactggggta agacgctgaa
    11461 agagatggat ttaaatctct ctttcgatct gcgtgtcgag ccactgagta aataccatct
    11521 tgatgatatt tccatgcacc gactgcgtgg cggcgaaatt gtcgccctgg acgatcagta
    11581 cacgtttttg cagcgtatcc cgcgcagcca ctacgtgctg gcagttggtc ctgttcctta
    11641 tctttattat ctccatcaga tgcgattgct ggatatcgcc ctgatcactt ttattgctat
    11701 ttccctcgcc tttccggtgt ttatctggat gcgtccgcac tggcaggata tgttaaaact
    11761 ggaagcagcg gcgcaacgat ttggcgatgg gcatctcaat gaacgtatcc actttgatga
    11821 gggttcgagc tttgaacgac ttggtatcgc atttaaccag atggcggaca atatcaacgc
    11881 cttgattgcc agcaaaaaac agcttattga cggtatcgct cacgaactgc gaacaccgtt
    11941 agtgcgcctg cgttatcgac tggagatgag cgataacctg agcgccgccg aatcccaggc
    12001 gttgaatcgt gatatcagtc aacttgaagc tttaattgaa gagctgctga cttatgcccg
    12061 actcgatcgc ccacaaaacg agcttcatct tagcgaacca gacctgccgt tgtggctgtc
    12121 aacgcatctg gcagacattc aggcagtaac gcccgataaa acggtacgga ttaaaacgct
    12181 cgcgcaaggc cattatgcgg cgttggatat gcgcttaatg gagcgcgtgc tggataattt
    12241 gctcaataac gccctgcggt actgccattc aacggttgaa accagcctgc tactgtcggg
    12301 gaatagagcg acattaattg ttgaggatga tggcccaggg attgccccag aaaaccgcga
    12361 acatatcttt gaaccttttg ttcgcctcga tcccagccgg gatcgctcaa ccggcggctg
    12421 cgggctgggg ctggcaattg tccactctat agcactggca atgggcggta cggttaattg
    12481 tgacaccagc gaactgggtg gtgctcgctt ctcgtttagc tggccgttat ggcataacat
    12541 cccgcaattt acctctgcct gacactacgc gcacgatggt caagtcacca ccactgtgct
    12601 ataaaataag tatgttgtaa ctaaagtggt taatattatg gcgcgttacg atctcgtaga
    12661 ccgactcaac actacctttc gccagatgga acaagagctg gctgcatttg ccgctcatct
    12721 tgagcaacac aagctattgg ttgcccgcgt gttctctttg ccggaggtaa aaaaagagga
    12781 tgagcataat ccgcttaatc gtattgaggt aaaacaacat ctcggcaacg acgcgcagtc
    12841 gctggcgttg cgtcatttcc gccatttatt tattcaacaa cagtccgaaa atcgcagcag
    12901 caaggccgct gtccgtctgc ctggcgtgtt gtgttaccag gtcgataacc tttcgcaagc
    12961 agcgttggtc agtcatattc agcacatcaa taaactcaag accacgttcg agcatatcgt
    13021 cacggttgaa tcagaactcc ccaccgcggc acgttttgaa tgggtgcatc gtcatttgcc
    13081 ggggctgatc acccttaatg cttaccgcac gctcaccgtt ctgcacgacc ccgccacttt
    13141 acgctttggt tgggctaata aacatatcat taagaatttg catcgtgatg aagtcctggc
    13201 acagctggaa aaaagcctga aatcaccacg cagtgtcgca ccgtggacgc gcgaggagtg
    13261 gcaaagaaaa ctggagcgag agtatcagga tatcgctgcc ctgccacaga acgcgaagtt
    13321 aaaaatcaaa cgtccggtga aggtgcagcc gattgcccgc gtctggtaca aaggagatca
    13381 aaaacaagtc caacacgcct gccctacacc gctgattgca ctgattaatc gggataatgg
    13441 cgcgggcgtg ccggacgttg gtgagttgtt aaattacgat gccgacaatg tgcagcaccg
    13501 ttataaacca caggcgcagc cgcttcgttt gatcattcca cggctgcacc tgtatgttgc
    13561 ggattaacgc ccggctttca tactgccgac catctgttct ggccgtaccc agctgtcaaa
    13621 ctcggcttcg ctaagatacc ccagcgcaag ggccgcagct tttaaggtca gcccttcttt
    13681 atgcgctttt ttggcgatct ccgcggcttt gtcataacca atgtgggtgt taagcgcagt
    13741 caccagcatc agcgattcat tgagtaattg attgattcgc tcacgattcg gttcaatacc
    13801 cactgcgcag tgtttgttaa aactttccat gccatctgcc agcaagcgca ccgattgcag
    13861 gaaattgtgg atcaccattg gacggaagac gttcagttca aagttaccgg aagcgccacc
    13921 catgttgatc gccacgtcgt tccccattac ctgacagcag agcatggtta atgcttcaca
    13981 ctgggttggg ttcactttcc ccggcatgat tgagctgccc ggctcatttt ccgggattga
    14041 gatttcacca attccgcagc gcgggccaga ggccagccag cggacatcat tggcgatttt
    14101 catcagtgac gcagccaacc ctttcaacgc gccgtgcgcc tgaaccaggg catcacaggt
    14161 cgcaagcgct tcaaatttgt tcggcgcggt aacaaacggt gcacaggtaa tgactgccag
    14221 ttcatctgct acgcgacgcg catactccgg atgggtattt agtccagtac ccaccgctgt
    14281 accgccgaga gccagttccg ctacgtgagg caggctgtat tcgatatgtt tgagattatg
    14341 ctcgagcatc gctacccagc cggaaatctc ctgccccagt gttagcggcg tggcgtcctg
    14401 caagtgggtt cgaccgattt tgacgatatc ggcaaatgca cgcgattttt cactcagtgt
    14461 ctgtgtcagg gttttaacct gcggaatgag ttgcttgcgc agcgccagca gcgccgcaac
    14521 gtgcatcgcc gtcggaaaga catcgttgga actttggctt ttgttcacgt cgtcgttagg
    14581 gtgaacttta cgttccatcc cgcgcacgcc gccgagtaat tcactggccc ggttagccag
    14641 cacttcgttc atgttcatgt tactttgcgt gccggagccg gtctgccaga tagccagcgg
    14701 gaattcgtcg tcatgctgtc ctgccagtac ttcatccgcc gcctgccgaa tggcgctcgc
    14761 tttctcttca gacaacaagc ctaaatcttc attaactttt gccgctgcgc gcttggttag
    14821 cgccagcgca tgaatcagtg aggtgggcat tttctccgtc gaaatgcgga aatgctccag
    14881 cgagcgttga gtttgtgcgc cccacagctt atctgccggg acatcaatcg cccccatcga
    14941 atctttttcg ctgcgtactg tattcatggc ctgctcctca cctgattaat tttttctttc
    15001 tgttttgcct tcgttaagca acttttagct cacttattat ttactatttg ataacaaatg
    15061 tttggtcttt cgtgccatgt aaaaaaaccg ccccgaaggg cggctctgtt tatttcacac
    15121 agcgagtgca ctgtgtgagt tgtatctgct ggaagaagtc atttccttta tcatccacaa
    15181 ggataaacgc cgggaaatct tccacttcaa ttttccagat ggcttccatt cccagttccg
    15241 gatattcaac acattccagg ctcttaatac ttccctgcgc caatacagcg gccggaccac
    15301 cgatactgcc aaggtagaag ccgccgtgtt ttttacaggc atccgtcacc tgctggctgc
    15361 ggttgccttt cgccagcatg atcatacttc cgccctgcgc ttgcagttga tcgacataag
    15421 aatccatccg tccggcggtc gttgggccaa gagaaccgga ggcataacct tccggcgttt
    15481 tggccggacc cgcgtagtaa atcggatgat ctttaatgta ctgcggcagc ccttcaccgt
    15541 tatccatccg ctctttcagt ttggcgtgag caatatcacg accgacgata atcgtgccgt
    15601 taagcgataa gcgtgtagaa acgggatact gcgacaactg tgcgaggatc tctttcatcg
    15661 gacggttaag gtcaacgcgc accgcttcgc cttctcccgc tttacgcagc tcttcaggga
    15721 tatatttacc tggattatgt tccagttttt cgatccagat cccctgacgg ttgatcttcg
    15781 ctttgatatt acggtcagca gagcaggaaa cacccatacc aaccggacag gatgcgccgt
    15841 gacgtggcag gcgaatcacg cgtatgtcgt gagcgaagta tttaccacca aactgcgcac
    15901 ccagaccgag attttgcgct tcgatcagca attctttttc cagttccaca tcgcggaatg
    15961 cctgaccgtg ctcattccct tccgttggca gttcgtcata gtatttagcg gaagccagtt
    16021 tcactgtttt aaggttagtt tctgcagaag ttccaccaat tacgaacgca atatgatacg
    16081 gaggacaggc cgccgtaccc agcgtgcgca tcttctcaac caggtaattt ttcagtttcc
    16141 ccggcgttag taacgctttg gtttcctgat agagatacgt cttgtttgcc gaaccaccac
    16201 ctttggcgat acagaggaat ttgtactcgt cgccatcaac ggcataaaga tcgatctgcg
    16261 ctggcagatt ggtgccggta ttcacctctt tatacatatc cagcggcgcg ttttgcgagt
    16321 agcgcaaatt atcttcgata taagtgttat agacaccgcg cgccagcgcc gcttcatcac
    16381 agccaccggt ccatacacgc tgcccttttt taccaacaat aatcgcagtg ccggtatcct
    16441 gacaggttgg cagaacacct ttcgccgcga tgtcggagtt gcgcaggaat tgcagcgcca
    16501 catatttatc attttcgctg gcttccgggt cacgcagaat gtcggccacc tgttgttggt
    16561 gagccggacg cagcataaat gacgcatcat gaaacgcctg acgcgccaac agagttaacg
    16621 cttcgggtgc gactttcaaa atctcctgcc cttcaaattc agatacgcta acgtgttcgc
    16681 tggttagcag gtaatactca gtatcatctt ttttgagtgg aaaaggagcc tgataatgaa
    16741 agggtttgtt tgacattgtt ctctcactta ctgcctggtt tggttatgct ctgggcgggt
    16801 gttccgttgc cctgttaaaa gcgagtaaca atatcctaca cactttttta acaaaaactg
    16861 agactagtac gactttttgc ggctccaggt tacttcccgt aggattcttg ctttaatagt
    16921 gggattaatt tccacattaa aacagggatt gatcatgcaa aaactcatta actcagtgca
    16981 aaactatgcc tggggcagca aaacggcgtt gactgaactt tatggtatgg aaaatccgtc
    17041 cagccagccg atggccgagc tgtggatggg cgcacatccg aaaagcagtt cacgagtgca
    17101 gaatgccgcc ggagatatcg tttcactgcg tgatgtgatt gagagtgata aatcgactct
    17161 gctcggagag gccgttgcca aacgctttgg cgaactgcct ttcctgttca aagtattatg
    17221 cgcagcacag ccgctctcca ttcaggttca tccaaacaaa cgcaattctg aaatcggttt
    17281 tgccaaagaa aatgccgcag gtatcccgat ggatgccgcc gagcgtaact ataaagatcc
    17341 taaccacaag ccggagctgg tttttgcgct gacgcctttc cttgcgatga acgcgtttcg
    17401 tgagttttcc gagattgtct ccctactcca gccagtcgca ggtgcacatc cggcaattgc
    17461 tcacttttta caacagccta atgccgaacg tttaagcgaa ctgttcgcca gcctgttgaa
    17521 tatgcagggt gaagaaaaat cccgcgcgct ggcgatttta aaatcggcgc tcgacagtca
    17581 gcagggtgaa ccgtggcaaa cgattcgttt aatttctgaa ttttacccgg aagacagtgg
    17641 cctgttctcc ccgctgttac tgaatgtggt gaaattgaat cctggcgaag cgatgttcct
    17701 gttcgctgaa acaccgcacg cttacctgca aggcgtggcg ctggaagtga tggcgaactc
    17761 cgataacgtg ctgcgtgcgg gtctgacgcc taagtacatt gatattccgg aactggttgc
    17821 caatgtgaaa ttcgaagcca aaccggctaa ccagttgttg acccagccgg tgaaacaagg
    17881 tgcagaactg gacttcccga ttccagtgga tgattttgcc ttctcgctgc atgaccttag
    17941 tgataaagaa accaccatta gccagcagag tgccgccatt ttgttctgcg tcgaaggcga
    18001 tgcaacgttg tggaaaggtt ctcagcagtt acagcttaaa ccgggtgaat cagcgtttat
    18061 tgccgccaac gaatcaccgg tgactgtcaa aggccacggc cgtttagcgc gtgtttacaa
    18121 caagctgtaa gagcttactg aaaaaattaa catctcttgc taagcttatt aaaggcttat
    18181 aacaccttca ggcggccagt ccgcctgatt tcattttatg gataatcatt atgaataaat
    18241 cgctggtagc ggtaggcgtc attgttgcgc taggcgtagt ctggacaggc ggcgcatggt
    18301 atacaggcaa gaagattgaa acccatctcg aagacatggt cgcgcaggcg aacgcgcaac
    18361 tcaaactgac agctcctgaa tccaacctgg aagtgagtta tcaaaactat catcgcggcg
    18421 tattcagcag ccagttgcaa ctgttggtga aacccattgc cgggaaagaa aatccgtgga
    18481 ttaaaagcgg tcagagcgtc atcttcaacg aatcggttga tcatggtccc ttcccgcttg
    18541 cccagcttaa aaaactgaac ctgatcccgt cgatggcatc aattcaaacc acgctggtta
    18601 ataacgaagt aagcaaacca ctgtttgata tggcaaaagg tgaaacgcct tttgagatta
    18661 actcgcgcat tggttacagc ggtgattcca gttccgatat ttcgctcaag ccactgaatt
    18721 acgagcaaaa ggatgaaaaa gtcgccttta gcggcggcga gttccagtta aatgctgaca
    18781 gagacggcaa agccatctcc ctttccgggg aggcgcaaag tggtcggata gacgcagtta
    18841 acgaatacaa ccagaaagtg cagttgacct ttaataatct gaaaaccgac ggttccagca
    18901 cgctggcaag ttttggtgag cgcgtaggaa accaaaaact gtcactggaa aaaatgacca
    18961 tttcagtgga aggcaaagaa ctggcactgc tggaaggcat ggagatcagc ggtaaatcgg
    19021 atctggtcaa tgacggtaaa acgatcaata gccaactgga ttactcgcta aacagcctga
    19081 aggtacagaa tcaggatctg ggcagcggca agctgacttt aaaagtcggc cagattgatg
    19141 gtgaagcctg gcatcagttt agccagcaat ataacgcgca aactcaggcg ctgctggcac
    19201 agccagaaat tgccaacaat cccgaacttt atcaggagaa agtgacggaa gccttcttta
    19261 gcgccctgcc gctgatgttg aaaggcgatc cggtgattac tatcgcgccg ctaagctgga
    19321 aaaacagtca gggtgaaagt gcgctgaatc tgtcgctgtt cctgaaagat ccggcaacga
    19381 ctaaagaagc gccgcaaacg ctggcgcagg aagtagatcg ttcggttaaa tctctggatg
    19441 cgaaactgac cattccggtg gatatggcaa ctgagtttat gactcaggta gcgaagctgg
    19501 aaggttatca ggaagatcaa gcgaaaaaac tggcgaaaca gcaagttgaa ggtgcatcag
    19561 caatggggca gatgttccgt ctgaccacct tgcaggacaa taccatcacc accagcctgc
    19621 aatatactaa cggtcagata acgttaaacg ggcagaaaat gccactggaa gatttcgttg
    19681 gtatgtttgc gatgccagct cttaacgttc cggctgtacc ggcaattccg cagcagtaat
    19741 tcacggccaa aacgccggag aattttcact tcggcgtttt catctgaaaa caaaccagag
    19801 attgcctgat gcgctacgct tatcaggcct accttttcct gcaacacttt gaatttatga
    19861 gtttttgtag gctggataag gcgtttacac cgcatccggc ataaaaaacg cgcactttgt
    19921 caacaatctg aaacgccgga ggtttttctc tccggcgtta tttctgactt cagcataaag
    19981 tcatactgaa tactcatttt cacatcccag taattcaggc gatcgcccag cgtactgcca
    20041 tcgcgctttt cccgcccttt atcggcagca agaaagaaat tcatcccttg taacggccct
    20101 tcgctgaccc gatagttcat accacctaag atacccgcgc cttcaatccg cacatctcgt
    20161 ttactggatt catagccgcg agtcacaatc tgtgcaaatg tcagtgtaaa ttgcggcgtt
    20221 aaacgatagt tgacgcccaa ttgccaggac tgcattcctt cacggttacg atcaagactt
    20281 aatgaaaagg gatagccaat gtcggtatcc accacggcac cattcattgc atggcgcaaa
    20341 taaccaccac tgccaaatac gcttccccac ggcgcattcc aggtcaattg tccactaacc
    20401 aacgccgttt cattgggctg gctggtatta cgacttaaac cttcgagctg cgcgtaaaag
    20461 ccgagcaact cccctttcaa cgtataattc gggtcgagcg gtatgctttt ctcgagaata
    20521 ctttgtatct gattggaaac atctttctgt accgatgccc ccagagaata gcgactggta
    20581 tcatcatgcc aactggcggc taaaaaagaa cgtgcgcgat gtttatcgta ttttcccgtc
    20641 tggctgttat acaccccttc atcacgaata ccggttgttg aatcagtcca taaaaatact
    20701 cgtgaacgcc acattcccca cagatcaata tttggtgtta aagcccaata acctaatgct
    20761 ccttcagaaa ctgtcggtaa tgtacgttgc gaactgccag ccattaacgg aatacgataa
    20821 ggtaagctac cataactata atccgtcccg aagcgacctg atttaagcag gctattttca
    20881 ctgggtttta tttttactgc ccccagggca aagccaaaac ttttatcacc atcaagatac
    20941 caccgggtac tcatatcagc acgagcacct aatttataaa cataatatgc cccgccttca
    21001 acgccaataa tattggaata ataaccacta ttgaaatcga gcaatcctcc ctgtacccat
    21061 gcgtaaatat ctcttcccgc gccagcactg ggcttatccg ccctgcgcaa ttcgtttttc
    21121 agtctgatgc gtagtcccgc ctcatcgtca gccagttgcg ccgcataagc agagataagg
    21181 ccagaggcag ccgtcaggca aataacggcc atggccacta tttttctcat agttgcacct
    21241 ttgatgttct gattattttt attgattatt aattagtgat atcgctgatt aattgctgct
    21301 gcactttttt acgattatca atttcaacca cgatttcttt gaatttttta tccgtaagcg
    21361 gataaaacca gataataacg aatgccagta gcataaatcc gcaaggtact aaggcaattg
    21421 atgtgcggat gcccataata acttccggcg tttgcacctg attggcaata tatccgctta
    21481 accccaaaat aaaggcagga attgaacctc cgattgcctg accgcattta cgggtaaatg
    21541 agaatagtga ataggtgagc ccttcaattc gcacgccggt caggtattca ccgtattcta
    21601 cggtatcagc ttccagtgcc cacatcactg tcatagtaac gccctgacca attgaagcga
    21661 tggccaacgc aaccaacgcc accggcagtg accagacgga aacccagaag aatagcagat
    21721 aaccgcaggt tcccagcaaa gcgccaatca ggaaggtatt ctttttaccg atcctcgcga
    21781 ccatccccgg cacaagcggt gccgatgcca cagtaccaac caggttttgc accagtacca
    21841 gcacagtgaa cagcccggta tcatttaaca catagcgcac gtagaacaac gacgaggcgc
    21901 tgaccgcaaa ggtcgaaatc agcacacaca gcgcaccgat gcacaacata aacagcgggc
    21961 gattccgttt cagggtttgc aaactgatct tcaatgacgg ctgcgcaacg atacgtacca
    22021 cattctcacg cgtcgatttg aagcagatga agtaaagcac cattccggca atcgccagca
    22081 caatcgtcca gaaatggtat accgacacca tctcttccgg gctggagttc ttaatgctcg
    22141 gccctatcag aaatgccagg cagacaaagg tcaatgaagc ggcaatccca cgagccgcgc
    22201 ccagacgggc gcgggattgt ggttgttggg tcatcgcggt agcaagtgaa ccataaggaa
    22261 tattcaccag gctgtagcaa aacccgaggc ccatgtaggt caaatatgca tacactactt
    22321 tgcggctatg gctccagtcg gtcggtaccc agaataccag cacgctgaag atcattaacg
    22381 gcgcagtacc gaagagtaaa aacgggcgga attttcccca gcgggtattc acactgtcca
    22441 ccactcgtcc ggcaaagacg tcggcgaagg catcgaatac ccgcaccagt aacagcatgg
    22501 tgcccgccgc agcggcaccg acgccagcga cgtcggtgta gtaactcaac aggaagagca
    22561 cccccattgc gaaggcgaag ttattggcga cgtcaccgag gctgtagccg acgatggtgc
    22621 gccaggagag ttgttgattc attgtttgcc tccctgctgc ggtttttcac cgaagttcat
    22681 gccagtccag cgtttttgca gcagaaaagc cgccgacttc ggtttgcggt cgcgggtgaa
    22741 gatccccttc ttgttaccgc caacgcgcaa tatgccttgc gaggtcgcaa aatcggcgaa
    22801 attccatacc tgttcaccga cgacggcgct gacgcgatca aagacgcggt gatacatatc
    22861 cagccatgca cactgatact cttcactcca catgtcggtg tacattgagt gcagcccggc
    22921 taacgtatcc acgccgtatt cggtgatgat aatcggctga tgcagtttct cctgccaggc
    22981 cagaagttct ttttccagta ccttctctgc cgtttccaaa tcgccgcttt ggacatacca
    23041 tccgtaataa cggttcaggc acagcacatc aaagagatcg ctgatggtat cggtgtgagc
    23101 gtcgcagaac attacattga cgcaggtgat cggacgcgtc gggtcgagtt tacgcgttgc
    23161 ttccgccagt ggcgcgaaat attcccgtgc accttgcgga cgggtatccg gttcgttggc
    23221 aatactccac atcaccacgc ttgggtggtt tttgtcacgc gctatcagct ctttaatcgc
    23281 ctgtaagtgc gcttgctgag tttccccgtt gactgcctct tcgctgtaca gttctttcgg
    23341 cttgttgccc gcttcgaaac caatgcctaa agagaggtta aagccgacag cagcagtttc
    23401 atcaatcacc acgatgccat gttcatctgc ccagtcgagc atctcttcag cgtaagggta
    23461 atgcgaggta cggtaggagt tggccccaat ccagtccatt aatgcgtggt cgtgcaccat
    23521 cagcacgtta tcgaatcctt tgccacgcaa gtccgcatct tcatgacgac caaagccagt
    23581 aaagtagaac ggtttgtggt taatcaggaa ctgttcgccc ttcactgcca ctgaccggat
    23641 gccgacgcga agcgggtaga tatcacactc tgtctggctt ttggctgtga cgcacagttc
    23701 atagagataa ccttcacccg gttgccagag gtgcggattc accacttgca aagtcccgct
    23761 agtgccttgt ccagttgcaa ccacctgttg atccgcatca cgcagttcaa cgctgacatc
    23821 accattggcc accacctgcc agtcaacaga cgcgtggtta cagtcttgcg cgacatgcgt
    23881 caccacggtg atatcgtcca cccaggtgtt cggcgtggtg tagagcatta cgctgcgatg
    23941 gatcccggca tagttaaaga aatcatggaa gtaagactgc tttttcttgc cgttttcgtc
    24001 ggtaatcacc attcccggcg ggatagtctg ccagttcagt tcgttgttca cacaaacggt
    24061 gatacgtaca cttttcccgg caataacata cggcgtgaca tcggcttcaa atggcgtata
    24121 gccgccctga tgctccatca cttcctgatt attgacccac actttgccgt aatgagtgac
    24181 cgcatcgaaa cgcagcacga tacgctggcc tgcccaacct ttcggtataa agacttcgcg
    24241 ctgataccag acgttgcccg cataattacg aatatctgca tcggcgaact gatcgttaaa
    24301 actgcctggc acagcaattg cccggctttc ttgtaacgcg ctttcccacc aacgctgatc
    24361 aattccacag ttttcgcgat ccagactgaa tgcccacagg ccgtcgagtt ttttgatttc
    24421 acgggttggg gtttctacag gacgtaacat aagggactcc tcattaagat aaaaatactg
    24481 gtcaaccttt aatctgatta gttaaccaat tctgtgttgc acacagttat ctgtgagaaa
    24541 cccatggaag agaaatggaa ataaaaagaa ctgacaccgc gatcaaaaaa ccaaaaactg
    24601 caatacccac agcgtgatat tgatcgcatt aatctttaaa acggcgttga cagaagccat
    24661 aaaataagca accgaacgtt cgttcgagca tattgggaac gataaactct catgacatat
    24721 ggctacggtg aatatttttg ggggggaatt ctccgatccc ggctagattg tattttgtac
    24781 agggaaatta aagtatgaaa cgcgggcaca gttatcacca gcccgcgaaa tgagaggatc
    24841 aggatgcggt taagataccg ccaatcatcg cgcgtaatcc ctgagcaaac gacgcttcac
    24901 gcgccagatc ttccgcttcg atatcggcca gtacaccgta ggtcagatcc agcagtaaac
    24961 gtgaagtcat tgacgtgttg atgtctgggc ttatctcgcc tttttgctgg gcatcacgca
    25021 tccgctgggc aacaaattcc gtgatcgtca tatgcttatt tttaagcatg gcggcgacct
    25081 gtgggttacg catcccttcc gccataattt cgaccaccag cgcccgttgc ccaaaggctt
    25141 catgggtgag agagacaata gactcgacca tatagtcaac aaaatgaatc cctttaatcg
    25201 gttcccggaa acgagccagc gccctctcct ggtcctgtaa gataatcgcc tgaatcaagg
    25261 cttctttgga gatgaaatgg tgatagagcg tcccgggact aatggcgcaa gatttacaga
    25321 tggctttcat cgaggcactg tgaaatccat tttctgaaaa aatctctctg gcagcattga
    25381 ggatccgggt ccgtgtcggt tgtgcttcag tctgcatgtt atccatcatt aaaccattat
    25441 agttagcgtg aataactagt ttaccgcgtt gtcaggtgag cgcctatcat tgctgttgaa
    25501 tagtgcgaag gcacactcta ttcgtatact tttcactctg aaattggtta accaatagcc
    25561 gtgatttggc tgccagttat ttagcagaga cgttgatgag tttatcccgg taaacaacac
    25621 gcacggttgt ttaccggtcc gttagtgtat taattgagct cctgtacccc accaccggag
    25681 acggtgagaa tttgtccgct tacccagctc gcagcaggcg agcaaaggaa cagcgctgcg
    25741 ttagcaatat cttgcggttg gcccagacgt ctgatcggcg tgtgctgtaa cattttttgt
    25801 tcaatttctg gtgtaataac ggatttcagg gcatcggtta atattgcccc cggcgcaatg
    25861 ccatttaccc gaatattttt ttcacccagg tcaaacgcca tatttctgac cagatgactg
    25921 gctgcagctt tagatgatgc ataggaagtc atgtttatat ttttattttc tgccgccata
    25981 gaagtgatgg tcagaataac gccaccgcca tttttttcca tttctggcgc aacaagttgt
    26041 gacagatgga aaaaagaaaa cacattcagt tcataagcac ggcgaaaatc cgccattggc
    26101 atatcaaacg gtttaggtcc accgccaccg gcgttgttaa ccagaatatc aactttaccc
    26161 agcttactga tagcaaagtc tgccagtgca gagagttcct gttcggaagt aatatcacaa
    26221 cggcaggcaa atgcctgacc acccagttgt tgaatttcgt ctacaacatg gttagctgcg
    26281 tcggcgttaa tatcactgac caccacagat gcgccagctg tcgcgaatgt aatggcgatt
    26341 tctttaccaa tacctgcacc cgcacctgtg atgatggcgc atggcgcatg gcgcatggcg
    26401 catggcgcat ggcgcatggc gcatggcgca ttttccgtc
//
LOCUS       DAJVRF010000046        26385 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJVRF010000046 DAJVRF010000000
VERSION     DAJVRF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26385
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.046"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..111
                     /locus_tag="OQN13_001750"
     CDS             <1..111
                     /locus_tag="OQN13_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCS1615128.1"
                     /translation="FQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
     gene            606..1505
                     /gene="lysO"
                     /locus_tag="OQN13_001751"
     CDS             606..1505
                     /gene="lysO"
                     /locus_tag="OQN13_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCS1615129.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1649..3301
                     /gene="hcp"
                     /locus_tag="OQN13_001752"
                     /gene_synonym="priS"
     CDS             1649..3301
                     /gene="hcp"
                     /locus_tag="OQN13_001752"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCS1615130.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            3313..4281
                     /gene="hcr"
                     /locus_tag="OQN13_001753"
     CDS             3313..4281
                     /gene="hcr"
                     /locus_tag="OQN13_001753"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCS1615131.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            4414..6132
                     /gene="poxB"
                     /locus_tag="OQN13_001754"
     CDS             4414..6132
                     /gene="poxB"
                     /locus_tag="OQN13_001754"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCS1615132.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            6169..7170
                     /gene="ltaE"
                     /locus_tag="OQN13_001755"
     CDS             6169..7170
                     /gene="ltaE"
                     /locus_tag="OQN13_001755"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCS1615133.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            7181..8611
                     /locus_tag="OQN13_001756"
     CDS             7181..8611
                     /locus_tag="OQN13_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCS1615134.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8710..9723
                     /locus_tag="OQN13_001757"
     CDS             8710..9723
                     /locus_tag="OQN13_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS1615135.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9720..10550)
                     /gene="amiD"
                     /locus_tag="OQN13_001758"
     CDS             complement(9720..10550)
                     /gene="amiD"
                     /locus_tag="OQN13_001758"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCS1615136.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10547..10870)
                     /locus_tag="OQN13_001759"
     CDS             complement(10547..10870)
                     /locus_tag="OQN13_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCS1615137.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(11081..12850)
                     /locus_tag="OQN13_001760"
     CDS             complement(11081..12850)
                     /locus_tag="OQN13_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCS1615138.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIA
                     LMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNIINNVFKNVSFRSVKINNVNTHSLKFINCHFFNVDMIRVNFSKCLFH
                     DCSMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSICARDIYNMSAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDKSIEILLLSCFAASDQKAIQRY
                     IPQSQDGEDFRSHLQYKLPLSAH"
     gene            13499..14014
                     /locus_tag="OQN13_001761"
     CDS             13499..14014
                     /locus_tag="OQN13_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1615139.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            14232..14960
                     /gene="artP"
                     /locus_tag="OQN13_001762"
     CDS             14232..14960
                     /gene="artP"
                     /locus_tag="OQN13_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCS1615140.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            14978..15709
                     /gene="artJ"
                     /locus_tag="OQN13_001763"
     CDS             14978..15709
                     /gene="artJ"
                     /locus_tag="OQN13_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1615141.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            15716..16432
                     /gene="artQ"
                     /locus_tag="OQN13_001764"
     CDS             15716..16432
                     /gene="artQ"
                     /locus_tag="OQN13_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCS1615142.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            16432..17100
                     /gene="artM"
                     /locus_tag="OQN13_001765"
     CDS             16432..17100
                     /gene="artM"
                     /locus_tag="OQN13_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCS1615143.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            17392..18123
                     /gene="artJ"
                     /locus_tag="OQN13_001766"
     CDS             17392..18123
                     /gene="artJ"
                     /locus_tag="OQN13_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCS1615144.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(18298..19425)
                     /gene="rlmC"
                     /locus_tag="OQN13_001767"
     CDS             complement(18298..19425)
                     /gene="rlmC"
                     /locus_tag="OQN13_001767"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCS1615145.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(19466..19954)
                     /locus_tag="OQN13_001768"
     CDS             complement(19466..19954)
                     /locus_tag="OQN13_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCS1615146.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(20014..20859)
                     /gene="potI"
                     /locus_tag="OQN13_001769"
     CDS             complement(20014..20859)
                     /gene="potI"
                     /locus_tag="OQN13_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCS1615147.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDGITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(20856..21809)
                     /gene="potH"
                     /locus_tag="OQN13_001770"
     CDS             complement(20856..21809)
                     /gene="potH"
                     /locus_tag="OQN13_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCS1615148.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(21819..22952)
                     /gene="potG"
                     /locus_tag="OQN13_001771"
     CDS             complement(21819..22952)
                     /gene="potG"
                     /locus_tag="OQN13_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCS1615149.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(23047..24159)
                     /gene="potF"
                     /locus_tag="OQN13_001772"
     CDS             complement(23047..24159)
                     /gene="potF"
                     /locus_tag="OQN13_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCS1615150.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(24510..24986)
                     /locus_tag="OQN13_001773"
     CDS             complement(24510..24986)
                     /locus_tag="OQN13_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCS1615151.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(25074..25976)
                     /gene="rimK"
                     /locus_tag="OQN13_001774"
     CDS             complement(25074..25976)
                     /gene="rimK"
                     /locus_tag="OQN13_001774"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCS1615152.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(26037..>26385)
                     /gene="nfsA"
                     /locus_tag="OQN13_001775"
     CDS             complement(26037..>26385)
                     /gene="nfsA"
                     /locus_tag="OQN13_001775"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCS1615153.1"
                     /translation="GVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWPADNPDLKPR
                     LPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRRTIIKESRPF
                     ILDYLHKQGWATR"
BASE COUNT         6521 a         6872 c         6521 g         6471 t
ORIGIN      
        1 ttccagggcg gctgggcatt agaacaactg tggttcttct gggtggtgcc aattgtcggc
       61 ggcattatcg gtggtctgat ttaccggacc ctgctggaaa agcgtgatta attaacgcag
      121 aaggcctggc gtaatgtcag gccttttttt aagatgttgt tatatgttga ttcatcatct
      181 tgttgcgctc ctttcatgag ctaagccaca tattgccact ggcgcaagga gcgcgcaggg
      241 ggcggccaat cgccgccgcc ccctgccccc ccgggctctg gcgaacaaaa tcgccgctgc
      301 gcggtgccct cgacttatcc cttacggcta ccgggtcggg cgcgaggtaa catccctgta
      361 aaacgcgccc tcagcccaca tccatgtggg ctgccccggc cttcagggaa cgcctcggcg
      421 attttgacgc caccaacatc ggtgcagttt gtaattaaag cgcaagaata tctttaaatg
      481 aaaacgaata ctctactgct ccttatgcat aacatttcct tttaattgca actttactcg
      541 tccttccgct ttcagtagtg tgtcatcgcg catttcgact tcatctttgc aaggactggg
      601 ttttcatgtt ttctgggctg ttaatcattc tggttcccct gattgtaggt tacctcattc
      661 cgcttcgcca acaagctgcg ttaaaagtta ttaatcagct attaagctgg atggtttacc
      721 ttattctctt ttttatgggt atcagtctgg cgtttctcga taacctcgcc agtaacctgt
      781 tggcgattct gcattattct gccgtcagta ttaccgttat tttactgtgt aatattgccg
      841 ccctgatgtg gctggagcga ggcctgccgt ggcgcaacca ccatcagcaa gaaaaactcc
      901 cgtcgcgtat tgcgatggcg ctggagtcgc taaaactgtg cggcgtagta gtgattggtt
      961 ttgccattgg tctgagtgga ctggctttct tacaacacgc gaccgaagcc agtgaataca
     1021 cgttaatttt gctacttttc ctcgttggta ttcagttgcg caataatggc atgaccttaa
     1081 agcagattgt ccttaatcgc cggggaatga ttgtcgccgt ggtggtggtt gtcagttcat
     1141 taattggtgg tttaattaac gcctttattc ttgatctccc catcaatacc gcgctggcaa
     1201 tggcctccgg tttcggctgg tattctcttt ccggtatttt attgaccgaa tcttttggtc
     1261 cggtaatcgg gagcgcggcg ttttttaatg atctggcccg tgaactgatt gctattatgt
     1321 tgatccctgg gctgattcgc cgcagccgct ctactgcact gggcttatgc ggtgccacat
     1381 caatggattt caccctgccc gttcttcaac gtactggcgg gctggatatg gtcccggcgg
     1441 caattgttca cggttttatt cttagcctgt tagtgccgat cctcatcgcc tttttctctg
     1501 cgtaatacct ctctggcggt agatccctgc cgccaaaatt gcgctaaatc aatctccctt
     1561 aaagttgcat gaaaaatccc ttttatcccc gcgttaagcg tcttaacctt aaacatgtat
     1621 attaaatata actttaaaag gtgtgatcat gttttgtgtg caatgtgaac aaactatccg
     1681 tactccggca ggaaacggct gctcatacgc gcaggggatg tgtggtaaaa cggcggaaac
     1741 ttctgacctt caggatttac tcatcgcggc gctgcaaggg ctttctgcct gggcggtaaa
     1801 agcgtgtgaa tacggcatca tcaaccacga tgttgacagc ttcgcgccac gtgccttttt
     1861 ctcaaccctg accaacgtta acttcgattc tccgcgtatt gtcggctacg ctcgtgaagc
     1921 gattgccttg cgcgaggcgc tgaaagcaca atgcctggct gtagatgcca acgcgcgcgt
     1981 cgataacccg atggctgacc tgcaactggt gagcgatgat ctcggcgagc tgcaacgtca
     2041 ggcagcagaa tttaccccta acaaagataa agcggcgatt ggcgaaaaca ttctcggcct
     2101 gcgtctgctg tgcctgtatg gcctgaaagg tgcggcggcc tatatggaac acgcgcatgt
     2161 tctcggtcaa tacgacaacg atatttatgc ccagtaccat aaaatcatgg cgtggctggg
     2221 gacctggcct gccgatatga acgcgcttct tgagtgttca atggaaatcg gccagatgaa
     2281 cttcaaagtg atgagcattc tggatgcagg cgaaaccggt aaatacggtc acccgacgcc
     2341 aacccaggtc aacgtcaaag cgacggcggg taaatgcatt ctgatttccg gtcacgatct
     2401 caaagatctc tacaacctgc tggaacagac cgaaggcacg ggcgttaatg tctacaccca
     2461 cggcgaaatg ctgcctgcgc atggctaccc ggagctgcgt aaattcaagc atctggtcgg
     2521 taactacggc agcggctggc agaatcagca agtggagttc gctcgtttcc ctggccccat
     2581 cgtgatgacc tcgaactgca tcatcgaccc aaccgtaggc gcttatgacg atcgtatctg
     2641 gacccgcagc attgttggct ggcctggcgt gcgtcatctg gacggtgatg atttctctgc
     2701 ggttatcacc caggcgcaac agatggcagg cttcccgtac agcgaaattc cgcaccttat
     2761 caccgtgggt tttggtcgcc agacgctgct tggcgctgct gatacgctga ttgatctggt
     2821 gagccgtgaa aaactgcgtc atatcttcct gcttggtggc tgtgacggcg cacgcggcga
     2881 gcgccactac ttcaccgatt tcgccaccag cgtgccggat gactgcctga tcctgaccct
     2941 cgcctgtggt aaatatcgct ttaacaaact tgagtttggc gatatcgaag gtctgccgcg
     3001 tctggtagat gcaggtcaat gtaacgatgc ttactcagcg attattctgg ctgtcactct
     3061 ggcagagaaa ctgggctgtg gtgtgaacga tctgccgctg tcgctggtgc tctcctggtt
     3121 tgaacagaaa gcgatcgtca ttctgctgac gctgctttct ctgggcgtga aaaatatcgt
     3181 caccggcccg actgcacctg gtttcctgac accagacctg ctggcggtgc tgaacgagaa
     3241 attcggcctg cgttctatca ccactgttga agaagacatg aagcaactgt tgagcgcgta
     3301 aggagtttgt cgatgacgat gccaacgaat caatgcccgt ggcggatgca ggttcatcac
     3361 attacgcaag aaacgccgga tgtgtggacg atttccctga tttgccacga ttactaccca
     3421 tatcgcgccg ggcaatatgc actggtcagc gtgcgaaact cagcggaaac gctgcgtgct
     3481 tacaccattt cctccacgcc aggcgtgagt gaatatatca ccctgaccgt gcggcggatt
     3541 gatgacggtg tcggctccca gtggctgacg cgcgatgtaa aacgcggtga ttatctctgg
     3601 ctttcggacg cgatggggga atttacctgc gacgataaag cagaagataa attcctgttg
     3661 ctggcggcag gctgcggcgt cacgccgatt atgtcgatgc gtcgctggct tgcgaagaac
     3721 cgtccacagg ccgatgtgcg ggtgatctac aacgtgcgta cgccgcagga tgttattttc
     3781 gccgatgagt ggcgtaacta tccggtaacg ctggtggcag aaaataacgt taccgaaggc
     3841 tttatcgctg gtcgtctcac tcgcgaactg ctggcaggtg tacctgactt agcttcacgt
     3901 accgtgatga cctgcggccc ggctccgtat atggattggg tagagcagga agtgaaagcg
     3961 ctcggcgtga cgcgtttctt taaagagaaa ttcttcactc cagtagcaga agcggcgacc
     4021 agcggtctga aattcaccaa actgcaaccg gcacgagaat tttacgcccc ggttggcacc
     4081 acgctactgg aggcgctgga aagcaataac gttccggttg tcgctgcctg ccgtgcgggt
     4141 gtttgcggct gctgtaagac aaaagtggtt tccggtgaat atacggtgag cagtacaatg
     4201 acgctgaccg acgccgaaat cgctgaaggt tacgtactgg cctgctcctg ccatccgcag
     4261 ggggatttgg ttctcgcata atcgccttat gcccgatgat attcctttca tcgggctatt
     4321 taaccgttag tgcctccttt ctctcccatc ccttccccct ccgtcagatg aactaaactt
     4381 gttaccgtta tcacattcag gagatggaga accatgaaac aaacggttgc agcttatatc
     4441 gccaaaacac tcgaatcggc aggggtgaaa cgcatctggg gagtcacagg cgactctctg
     4501 aacggtctta gtgacagtct taatcgcatg ggcaccatcg agtggatgtc cacccgccac
     4561 gaagaagtgg cggcctttgc cgctggcgct gaagcacaac ttagcggaga actggcggtc
     4621 tgcgccggat cgtgcggccc cggcaacctg cacttaatca acggcctgtt cgattgccac
     4681 cgcaatcacg ttccggtact ggcgattgcc gctcatattc cctccagcga aattggcagc
     4741 ggctatttcc aggaaaccca cccacaagag ctattccgcg aatgtagtca ctattgcgag
     4801 ctggtttcca gcccggagca gatcccacaa gtactggcga ttgccatgcg caaagcggtg
     4861 cttaaccgtg gcgtttcggt tgtcgtgtta ccaggcgacg tggcgttaaa acctgcgcca
     4921 gaaggggcaa ccacgcactg gtatcatgcg ccacaaccag tcgtgacgcc ggaagaagaa
     4981 gagttacgca aactggcgca actgctgcgt tattccagca atatcgccct gatgtgtggc
     5041 agcggctgcg cgggggcgca taaagagtta gttgagtttg ccgggaaaat taaagcgcct
     5101 attgttcatg ccctgcgcgg taaagaacat gtcgaatacg ataatccgta tgatgttgga
     5161 atgaccgggt taatcggctt ctcgtcaggt ttccatacca tgatgaacgc cgacacgtta
     5221 gtgctactcg gcacgcaatt tccctaccgc gccttctacc cgaccgatgc caaaatcatt
     5281 cagattgata tcaacccagc cagcatcggc gctcacagca aggtggatat ggcactggtc
     5341 ggcgatatca agtcgactct gcgtgcattg cttccattgg tggaagaaaa agccgatcgc
     5401 aagtttctgg ataaagcgct ggaagattac cgcgacgccc gcaaagggct ggacgattta
     5461 gctaaaccga gcgagaaagc cattcacccg caatatctgg cgcagcaaat tagtcatttt
     5521 gccgccgatg acgctatttt cacctgtgac gttggtacgc caacggtgtg ggcggcacgt
     5581 tatctaaaaa tgaacggcaa gcgtcgcctg ttaggttcgt ttaaccacgg ttcgatggct
     5641 aacgccatgc cgcaggcgct gggtgcgcag gcgacagagc cagaacgtca ggtggtcgcc
     5701 atgtgcggcg atggcggttt tagcatgttg atgggcgatt tcctctcagt agtgcagatg
     5761 aaactgccag tgaaaattgt cgtctttaac aacagcgtgc tgggctttgt ggcgatggag
     5821 atgaaagctg gtggctattt gactgacggc accgaactac acgacacaaa ctttgcccgc
     5881 attgccgaag cgtgcggcat tacgggtatc cgtgtagaaa aagcgtctga agttgatgaa
     5941 gccctgcaac gcgccttctc catcgacggt ccggtgctgg tggatgtggt ggtcgccaaa
     6001 gaagagttag ccattccacc gcagatcaaa ctcgaacagg ccaaaggatt tagcttgtat
     6061 atgttgcgcg caatcatcag cggacgcggc gatgaagtga tcgaactggc gaaaacgaac
     6121 tggctaaggt aaaaagggtg gcatttcccg tcataataag gacatgccat gattgattta
     6181 cgcagtgata ccgttacccg accgagccgc gccatgctcg aagcaatgat ggccgccccg
     6241 gttggggacg acgtttacgg agacgaccct accgttaatg ctctgcagga ctacgccgca
     6301 gagctttccg gtaaagaagc cgccattttt ctgccgaccg gcacccaggc taacctggtc
     6361 gctctgctca gtcactgcga acgcggcgaa gagtatattg tcggtcaggc cgcgcataat
     6421 tatctgtttg aagccggtgg cgcagcggtg ctgggcagta ttcaaccgca acccatcgac
     6481 gcggctgccg acggcacgct accgctggat aaagtggcga tgaaaatcaa acccgacgat
     6541 atccatttcg cccgcaccaa attactcagt ctagaaaaca cccacaacgg caaagtgctg
     6601 ccgcgtgaat acctgaaaga agcatgggaa tttacccgca agcgcaatct ggcgctgcat
     6661 gttgacggtg cgcgcatctt taatgccgtg gtggcttacg gctgcgaact gaaagagatc
     6721 acgcaatatt gtgattcgtt caccatttgc ctgtcgaaag gtcttgggac gccagtcggt
     6781 tcattactcg tcggtaatcg tgattacatt aaacgtgcca tccgctggcg gaaaatggcc
     6841 ggtggcggga tgcgtcagtc cgggattctg gctgccgccg ggatgtatgc cctgaaaaat
     6901 aacgtcgcac ggttgcagga agatcacgac aacgccgcct ggatggcgga gcaactgcgt
     6961 gaagcaggcg cggatgtgat gcgtcaggac actaatatgc tgtttgttcg cgtcggggaa
     7021 gaaaatgctg ccgcgttagg cgaatacatg aaagcgagaa acgtgctgat taacgcctcg
     7081 ccgattgtcc gcctggtgac gcatcttgac gtctcgcgcg aacaactggc ggaagtcgcc
     7141 gcccactggc gcgcattcct ggcgcgttaa ggagagagac gtgccgcaac gcattttagt
     7201 tctcggtgcc agtggctaca ttggtcagca tctggtgcgc acactcagcc agcaagggca
     7261 tcagatcctg gcggcggcac gtcatgtcga caggcttgca aagctgcaac tggcaaacgt
     7321 cagttgccat aaagtcgatc tcagctggcc ggataacctt ccggccctgt tgcaggatat
     7381 cgataccgtc tattttctgg tgcacagcat gggcgaaggc ggcgatttta tcgctcagga
     7441 gcgccaggtg gctctcaacg tccgcgatgc gctacgtgaa gtaccagtta agcaattaat
     7501 ctttctcagt tcgttgcagg ccccgccaca tgagcagtcg gatcatctgc gtgctcgtca
     7561 ggctacggcg gacattcttc gtgaagcgaa tgtacctgtg accgaacttc gggccggaat
     7621 tatcgttggc gcaggttcag cggcgttcga agtcatgcgc gatatggtct acaacctgcc
     7681 agtgttaacg ccgccacgct gggtacgttc acgcaccacg cccatcgcgc tggaaaactt
     7741 gctgcactat ctggtggcgt tgttagatca tccggccagc gaacaccgca tcttcgaagc
     7801 cgccggacca gaggtgctca gttatcagca acagtttgaa cattttatgg cggtgagcgg
     7861 taagcgccgc tggttgatcc ccatccccct ccccacccgc tggatttcgg tgtggtttct
     7921 caatgtgatt acttccgtac cgcccaccac cgccagggcg ttgattcagg ggctgaaaca
     7981 cgatctgctg gcggatgata ccgcgctacg tgcactcatc ccacaacggc tgatcgcttt
     8041 cgatgacgcg gtacgtagca cgttgaaaga ggaggaaaaa ctggtcaact ccagcgactg
     8101 gggctacgac gctcaggcct ttgcccgctg gcgaccggag tacggttatt ttgccaaaca
     8161 ggcggggttt accgttaaaa cgtccgccag ccttgctgct ttatggcagg tagtgaacca
     8221 aatcggcggt aaagagcgtt atttctttgg caatattttg tggcagacac gggcgttgat
     8281 ggaccgcgcg atcggtcata agctggcgaa aggccgcccg gagcgcgaat atttacagac
     8341 tggcgatgcg gtggatagct ggaaagtgat tgtcgttgaa ccggaaaaac aacttacgtt
     8401 gttatttggc atgaaagcgt cggggctggg acgactgtgt tttagcctgg aagataaagg
     8461 cgactatcgt actatcgatg tccgcgcttt ctggcatccg cacggtatgc cggggctgtt
     8521 ttactggtta ttgatgatcc ccgcgcatct gtttattttt cgcggaatgg caaaacaaat
     8581 cgccagactg gcagaacaaa gcacagatta atcgacaaaa cctgcgttat tctttcatgt
     8641 ttcccattgc atcatggggg aaatcacgga agaatgcgca cgtcattttc tcaacaacag
     8701 tggattgata tgaaggtact ggttaccggc gccaccagcg gcttaggtcg aaacgcggta
     8761 gagtttttat gccagaaagg catcagcgtg cgagcgaccg gtcgcaacga ggcaatgggc
     8821 aaattgctgg aaaaaatggg cgcagagttt gttccggcgg atctgaccga gctggtttca
     8881 tcacaagcta aagtgatgct cgcgggcatt gatacgctgt ggcactgctc cagctttacc
     8941 tcaccctggg ggacacaaca ggctttcgat ctggctaacg ttcgcgccac tcgccgcctg
     9001 ggtgaatggg ctgtcgcctg gggtgtgcgt aactttattc atatctcttc cccctccctg
     9061 tacttcgatt atcaccacca tcgcgatatt aaagaagatt ttcgccctca ccgcttcgcc
     9121 aacgagtttg cccgcagcaa agcagccagc gaagaagtga tcaatatgct ttcgcaggcg
     9181 aatccacaaa cgcgctttac tattctgcgc ccacaaagtc tgttcggacc gcacgataaa
     9241 gtctttattc cccgtctggc gcatatgatg caccactacg gcagtattct gttaccgcat
     9301 ggcggcagtg cgctggtcga tatgacctac tatgaaaata ccgtgcacgc aatgtggctg
     9361 gcaagccagg aagcctgcga taagctacct tccgggcgtg tgtacaatat caccaacggc
     9421 gagcatcgca cactgcgcag catcgtgcag aagctgatcg acgagttgaa tattgactgt
     9481 cgtattcgtt ccgtccccta cccgatgctg gatatgatcg cccgcagcat ggagcgttta
     9541 ggccgcaagt cagcaaaaga gccgccgctg acccactacg gcgtctccaa gttgaatttt
     9601 gactttacgc tggatattac gcgtgcgcag gaagagttag gttatcagcc gatcatcacc
     9661 ctagatgaag gtatcgagaa aactgccgcc tggctacgcg accacggaaa actgccgcgc
     9721 taatcctgcc cgtatttctc cagcaatgct tcggcaatcg cctgagtttc tgcatccgct
     9781 tcgccgttat ataacgtcgg gcggaaatgc atctggaatg ccataatcac gcgccgctgc
     9841 tcgcgcggtg tcatatcagg tttaacgtca taaccgtagc gcgccaaaag ctccagcaat
     9901 gacgcggtat ctaccggtgt gtgcggcgcg cgcccggcaa ggtaaaagtt aacccgctgc
     9961 gcatccggcc aggcaccaat cccctgctgc gccagttgct gccagggaaa taatggcccc
    10021 ggatcgtctt tgcgctgcgg tgcgatatcc gcatgtgcca ctacgttttc cggcttgatg
    10081 tgataacggg caataatatc tttcgccagt ggaataagcg cctgaatctg tgtcggttca
    10141 aacggggcaa aatatttcac tccggccgat ttttgccagc cacggttttc cagctcaatg
    10201 ccaatagagg tgtcgttaag gcgcgttgcc ccgcgccagg cgctaatccc cgcatgccag
    10261 gccagttctt gttccggcac cagttgccag atgcgcggtt taccgttgta tcgcggtggt
    10321 accgcaggga ccagataatg cgagctgacc tgcttatcgg tcagtgtcgc cagcgagcta
    10381 tcaaaatcat ctgcggtgta gtggatcacc agcactttaa tgcgcggata cgccgcctgc
    10441 gcctggcgtc gggtatcaag ctgatatccc tctttctcga caatgccttt ttcgcctgca
    10501 caccctgcca ataacagagc ggcagcgacc agccagaaga ctcttctcat cgacgcgttt
    10561 tcaccgccgt accgctgacg ctaaccatca gcatactgcc gttttgcccg accgtttcgt
    10621 agtcgatatc aataccgacg acggcatcgg cccccagcgc ccgcgcctgg gagcctaatt
    10681 cctcaaaggc gatctcccgt gctttacgca gttctttttc atacgcaccg gaacgtccgc
    10741 caacgatatc gcggataccg gcaaagaaat cacggaaaat attggcacct aaaatcgctt
    10801 cgccggtcac cacaccgcaa tattcaacga tggtctggcc ttccagagtt ggggttgtag
    10861 aaaattgcat gatcgatctc cttgtttgtt aagccttcga tctctaaagc attatcgaac
    10921 tgatatgcca tgttgggaag aaggttttga tcagtttagg aaatcatctc attgtgtcaa
    10981 aaacacccac ttaagtcttt acgattatcc gttgcataac ccgatattgg tttcattata
    11041 gggtaccgct tacctgtctt attatgttgc accaacaaca ttaatgagcg gaaagaggca
    11101 acttatattg taaatgactc ctaaagtctt ccccatcttg tgactgaggt atatacctct
    11161 ggattgcttt ttggtcagac gccgcaaaac aagaaagtaa taagatctca atgcttttgt
    11221 cattaatata agcgaagatc attcttgcaa attcgtaaat aaacgcccgt tcctgagcgc
    11281 ttaatagttt aatgcaatat ttattcttaa gagagaaaat aatttcaagg aaagcatccg
    11341 cagcaacctt cacgttattc ccggcgctca tattatatat atcccttgca cagatagagt
    11401 acagagatag taaacagcca tacaaataat catttattaa ctcttttgtc cattttactg
    11461 gcaaccatgg ttttatcttg acaccatgca tactacagtc atgaaaaaga catttgctga
    11521 aattaactct tatcatatca acattaaaaa aatggcaatt aataaatttt aaagaatgag
    11581 tatttacgtt gttaatttta acagatctaa acgaaacatt tttgaataca ttatttatta
    11641 tattgctatt tgttatttta acatcaccta aactgccgtt ttcaaaacaa cattttttca
    11701 atatgctttc attcacaaca gtgtgtgata agtagagatt acttatgttt tgctttatta
    11761 atgccacgtc attaaaatga ctatatttaa tgaaaaaacc atcattgagt agtgttttaa
    11821 gattgactaa attacttgca tcttcacgta ccatcgtggc aactggaaca ggcagagaca
    11881 tgaaaacttc ttcattcacc atcaagtgaa tcatgcattg cgttcccttt gtcatatcca
    11941 cacatacgct acaaatatcg tggtaatgcg aagagcgcat ttcaggaaac agattaaaca
    12001 cggtatcttg aaaggcacta aatcgtgtta ataaatcctg ataatgaaca ggttttgttt
    12061 caaaattatc atcagcaaat ccaagcacta ttgtatgctc tacatctaac gcagtttcaa
    12121 atggctcttc ttctgtaaaa tcattattta aaacctcacc tgacgtatta tcactgtcat
    12181 aaaaagtgtt catcaatgcg atattgtttg ttacagcgtc tttttcgata ttctcactaa
    12241 ttgcatatcg cctgctataa tcataaaaat gaataaacgc gccctttata aaggatatat
    12301 attgttcttt gttaaagtca atacggctgt ataatttttc aaaaatcaca ttcgcaattt
    12361 tactaccaat ggtatgcctg tataataatg ggtgcgattc aatcgtaaaa tgcttgtcta
    12421 attgatcttg taatttattc tcatagtcca gagggaaaag aaactgttgt ctttcaagtt
    12481 tcccattcga tacattacgt attccaatag agattctttc attacccgcc tttatttcaa
    12541 cttcgttatc ttctgattta cccagatatt ttttaacatc agtctcgaaa gcattactaa
    12601 aaaaagtatt aaattggtaa ttcgctgatg gcatattttc cgcgagcaac acatcatttt
    12661 ttatgagatc actgtcagca tttattgcta atatcagatg caagtagatg gtatcccatg
    12721 tagacaactc acccttacga ctggaattta atatcgcgaa aaattccctc agtgaatcca
    12781 ggaaattatc actttgattt agttgtgtgc ctggaaatga tgatgcttga tcagatatat
    12841 tgtttatcat attaaaaatc acccaagaaa aacgataatc caccgaactc ctcgtgcttt
    12901 ataattataa aagcacaacg ccttttatat tcatattaat taacgagata ctgaaacaac
    12961 ttaatttatt cttcgctaaa attcattagc ttaaattctt tagccacaat tgtagcataa
    13021 ataatattat cgagagtcaa agctgggact atcaaatttc gctcattttc aaaaatacaa
    13081 cgtaattttg gcaatcatct taatcgcagg atctcttatc gcatgtgaaa aattttgtaa
    13141 tgattagaca ataaccgcgt tcatttatca cgcgttataa atgtgaaact ggcttcttgc
    13201 agatgctctt aacctctaca ggaggattta taccaccatt tcagaactat catatttacc
    13261 ttaaatgatc atttcagcca tataacacgc tcaacctcac aaatggttta cactgtctat
    13321 tgtttgggta atttaagtaa gtcagctacg agcaaccttg agtggatagc cagcaatgga
    13381 ctcaaaccca atcaggtgtt cagattgttt cagaattgta ttaaaaacaa tgtattaaat
    13441 aatctgatag cgtttggtcg tagtgtgacg cagacagcaa gctaataagg aaatgattat
    13501 gcgctaccgt tcactatctc tacttattcc atgtgcactg ctgctgagtg cctgtaccac
    13561 cgtcacgcca gcttataaag ataacggtcc ccgcaccggt tcctgtgtcc agggggggcc
    13621 tgacagcgtg gctcaacaat tctatgacta ccgcattcaa catcgcagca atgacattac
    13681 cgccctgcgc ccctatttga gtgacaaact ggcaaccctt cttagcgatg ccagccgcga
    13741 taatagtcat cgtgaattgt taagcagcga tccgttctcc agtcgaacca ccttgccaga
    13801 tagcgcccac gttgccagtg catcgactat ccctaatcgt gatgcccgta acattccatt
    13861 gcgcgtagat ctcaaacagg gtgaccaggg ttggcaggat gaagtgttaa tgattcagga
    13921 aggccagtgt tgggtcatcg acgatgtgcg ctacttaggc ggcagcgttc atgccacggc
    13981 gggtacgctc cgtcagtcca tcgagaatcg ctaacgcctt gatcaaccaa taaaccccgt
    14041 aaattgtccg gcatttcgtg aggaatgccg acatttatgc tcgccgacca ccgcccccgt
    14101 tattttgtgc tatgtttatt gaataatgcg ctttgctttt aacttttaaa gcagaaatat
    14161 tgcataatta ttctgtcaaa ggtactatct tcgacctcaa tagctatcag actgccagta
    14221 tacgagtgtc aatgagtatt caattaaacg gcattaattg cttctacggc gcgcatcagg
    14281 cgctgttcga tatcacgctg gattgcccac agggcgaaac gctggtgtta cttggcccca
    14341 gcggcgcggg taaaagctcg ctgctgcgtg tactcaatct gcttgagatg ccacgctccg
    14401 gtacgctcaa cattgcaggc aaccatttcg atttcaccaa aacaccctct gacaaagcga
    14461 ttcgcgattt gcgtcgtaac gttggcatgg tgtttcagca atacaacctg tggccgcatc
    14521 tgaccgtgca gcaaaacctg attgaagcgc cctgccgtgt actggggttg agtaaagatc
    14581 aggcgctggc ccgtgcagaa aaactgctgg aacgtctgcg tctcaaacct tatagcgatc
    14641 gttacccgct gcatctttct ggtggtcagc agcagcgtgt tgctattgcc cgtgcgttga
    14701 tgatggaacc gcaggtactg ctgttcgatg aaccgaccgc cgcactggac ccggaaatta
    14761 cggcacaaat cgtcagcatc attcgtgagc tggcagaaac gaatattacc caggtgatcg
    14821 tcacccacga agttgaagtg gcgcgtaaaa ccgccagccg agtggtgtat atggaaaatg
    14881 gtcatatcgt agaacaaggc gacgcgagct gctttaccga gccgcaaacc gaagcattta
    14941 aaaactatct ctctcactaa gattcgggat aacaacaatg aaaaaagttc tgattgccgc
    15001 gttaattgca ggttttagtc tttccgccac agctgccgaa accattcgtt ttgctaccga
    15061 agcatcctat cctccgtttg aatcgattga tgcaaacaac cagatcgttg gttttgacgt
    15121 cgacctggca caagcgctgt gtaaagagat tgatgcaacc tgcactttct ctaaccaggc
    15181 gtttgacagc ctgatcccaa gcctgaaatt ccgtcgcgta gaagccgtga tggcgggcat
    15241 ggatatcact ccggagcgtg aaaagcaggt gctgtttacc accccgtact atgacaactc
    15301 tgccctgttt gtgggtcagc aaggcaaata caccagtgtt gatcagctga aaggcaaaaa
    15361 agtcggcgta cagaacggga cgacacacca gaaattcatt atggataagc acccggaaat
    15421 cactaccgtt ccgtatgaca gctaccagaa cgcaaaactg gatctgcaaa acgggcgtat
    15481 cgacggcgtc ttcggtgaca ccgcagtggt cactgagtgg ctgaaagata acccgaaact
    15541 ggcggcggtg ggcgacaaag tgaccgataa agattacttc ggcactggcc tcggcatcgc
    15601 ggtacgtcag ggcaacactg agctgcagca gaaactcaac actgcgctgg aaaaagtgaa
    15661 gaaagatggc acttacgaaa ccatctacaa caaatggttc cagaagtaat tcctgatgaa
    15721 tgaatttttt cctttagcaa gcgccgccgg gatgaccgtc ggccttgccg tttgtgcatt
    15781 gattgtcggg ctggcgctgg cgatgttctt tgcggtatgg gagtcggcaa aatggcgtcc
    15841 tgtcgcgtgg gcaggttcag cgctggtaac cattctgcgt ggcctgccag aaattctggt
    15901 ggtgctgttt atctattttg gctcctcgca gctgctgctg acgctttcgg atggcttcac
    15961 tatcaatctt gggttcgtgc agatcccagt gcagatggac attgagaact tcgacgttag
    16021 cccgttcctt tgtggtgtca tcgctctgtc actgctgtat gccgcctatg cctcgcaaac
    16081 gcttcggggc gcgttgaaag cggtgccggt gggtcagtgg gagtctggtc aggcgctggg
    16141 gctgtcgaaa tcggctatct ttttccgtct ggtgatgccg cagatgtggc gtcatgcgct
    16201 gcctggcctc ggtaaccagt ggctggtgct gctgaaagat accgcgctgg tcagtttgat
    16261 tagtgtgaat gatttaatgc tgcaaacaaa aagcatcgct actcgtaccc aggaaccatt
    16321 tacctggtac attgtggcgg cggcgattta cctggtgatc accctgctca gtcagtacat
    16381 tctcaaacgc attgacctgc gcgcgacacg ttttgagcgg aggcccagct aatgtttgag
    16441 tatttacccg aactgatgaa agggctacac accagcctga cgctaaccgt tgcctcgctg
    16501 attgtggcac tgattctggc attgattttt accatcatcc tgacgctgaa aacgccggtg
    16561 ctggtgtggc tggtgcgggg ttatatcacg ctgtttaccg gtacgccgct gctggtgcag
    16621 atcttcctga tttattacgg gccgggccag tttccgacgt tgcaggagta tccggcactg
    16681 tggcatttgt tgtcagaacc gtggttatgt gcgctgattg cgttatcgct gaacagtgcg
    16741 gcgtatacca cgcagctgtt ttacggtgca attcgtgcga tcccagaagg tcagtggcag
    16801 tcctgtagcg ccctgggaat gagcaaaaaa gatacgctgg cgctcctgct gccgtatgcc
    16861 tttaaacgct cgctctcttc ttattccaac gaagtggtgc tggtgttcaa aagtacctct
    16921 ctggcttaca ccattacgct gatggaagtg atgggataca gccagttgtt gtacggacgc
    16981 acctacgatg taatggtgtt cggtgcggca gggattattt acctggtcgt caacggcctg
    17041 ctgacgctga tgatgcgtct gatcgagcgc aaagcactgg cattcgaacg gcggaattaa
    17101 tgtagctgcg tacattcgcc gggggggttt gtaggtctga taagacgcgc cagcgtcgca
    17161 tcagacatca gcacggtgcc tgtgccggat gcggcgtgga cgccttatcc ggcctacata
    17221 gtgcataaat tcttataatg aagacggaca actcactaag ttatccgtct ttttttattt
    17281 catttaaatt atttaatcat atttattgca tataaattca cttgatggca ttgttatccc
    17341 atgccgcaga cacggccaaa aatcataaga ttgacagacg ggagttccat catgaaaaag
    17401 ttagttcttg ccgctttact tgcttccttt actttcggtg cttctgccgc agagaaaatc
    17461 aattttggcg tttcagccac ctatccaccc tttgaatcta taggtgctaa taatgagatt
    17521 gtcggctttg atatcgatct ggcaaaagcc ttgtgcaaac aaatgcaggc agaatgtact
    17581 tttactaatc acgcgttcga cagcctgatc ccgtcactga aattcagaaa atatgactcc
    17641 gtaatctccg gtatggatat cacgcccgag cgtagcaagc aggtatcgtt taccacgccc
    17701 tactatgaaa actcagccgt cgtgattgcc aaaaaagata cctacaaaac gtttgccgat
    17761 ctgaaaggca aacgtattgg gatggaaaac ggcaccacgc accagaaata tattcaggat
    17821 cagcaccccg aagtgaaaac tgtctcctat gacagttatc agaatgcctt tatcgatctg
    17881 aaaaatggtc gtattgatgg ggtatttggt gacacagcgg tggtaaacga atggctgaaa
    17941 accaatccgc aactgggcgt tgctactgaa aaagtgaccg atccgcaata cttcggcacc
    18001 ggtctgggca tcgctgtacg tccggataac aaagctctgc tggaaaaact gaataacgcg
    18061 ctggcagcaa ttaaagctga cggtacatat cagaaaatca atgaccagtg gttcccgcag
    18121 taattgagac tgatgacaaa agtaagattg cctgatgcgc tgcgcttatc tggcccacat
    18181 caactctgca atgtattgat ttagcatgat tttgtaggcc ggataaggcg tacacgccgc
    18241 atccggcatg aacaaagcgc acctgatcag caatctgaag ccgcacctgc ggctttttta
    18301 ttgcttcacc agcagcgtca tcacttcata gtgcgcggtg tgcgggaaca tatcaaaaag
    18361 ctgtacccgt tcgatgcggt aacctggcag ttcgcggata tctttcgcca tggtttgggc
    18421 gttacagctg gagtagataa taaaacgcgg tgccatcgtt gagagataat cacacagcgg
    18481 tttaccaatg ccgcggcgcg gcgggttaac cagcaccagc tccggcacat ccccctgagc
    18541 ggtggcaaac tgagtggagt ccagcgcctg aaattgcaaa cgcgttaagc ccagttcagc
    18601 ggctgactgc tttgcacagg caatggcctc tgatgcaatt tcgatcccgg ttaactgcat
    18661 gtcaggcgtc gcgcagtgta aaccaaagcc acccacaccg cagaacagat cccacatatg
    18721 tttaactggc agctgccgta cccagtcgcg cgcggtagcg tacagctggc tggcgaccgc
    18781 cggattagtc tggaagaaac tttgcggacg gatccacagc ggtacgtcat taaaacgctc
    18841 tgccagcgcc tgttgttcag tcaggtagat ctccgtctcc ccttccataa tcgccatatg
    18901 taccggctga atattgacgg taataacttt cagttgcggt agttgttcct gtaaccacgg
    18961 cagcgcctta cgcagttgtg ccagtttggt ttcagaacgc agtacaaagc gcagcatcat
    19021 gccgccatcg ctctggcttt cagtcagcag aatgtatttc agttcgccac gcttacgcgc
    19081 cacgttgtag ggcgttaacc ccgcacgggc gataaacggt tttagcgccg caaaaacggg
    19141 cgcaaaagag gcaggataaa gcgggcagtc acaaaggtct tccggcgtgc catcgcgatg
    19201 cagcataccg agcagtggtt tttcaacgct accactcacc accattttgg ctttattacg
    19261 aaacccttgt tccgggcctg acaccggcgc gcaccattcc tcaaccggaa aatcggcgag
    19321 cagattttta agatcggcgg ttttagcgga gagttgctct ggaatcggct gcgttatcca
    19381 ctgacaggaa cgacagcgac ccgcgtcgta aagtgcgcac tgcatacatt gaccttcaca
    19441 tcatcagggg cgacgattat acacattatt gcaactggaa gaaccgccga ctggtcgagg
    19501 gaacgaacag cagcatcagg atgagcatat ccggcagctt ctgcagcatc aggctatgga
    19561 agatttcacg ttttgattca ccgggaatgc tgaacagctc cggataaccg taccccagcg
    19621 aagccgccca caagtaactt gcggcggtga tttgtgtcag cagatatagc cagcgcgccc
    19681 agcgacgccc tttcaccagt gaaaacgcac accagatctc aatgaaaacc agcaccagac
    19741 tgcttaaaaa gaccagcgtt aaactccagg tttgtacgct gcgatgaatg aactcgccaa
    19801 taccgcgcac gcccagcgta ttgaaaatca tcagcacgtc gaggccacgg atcataataa
    19861 tggcgagcgc cgccacctgc accagcgcag gcacattcag gcgagcatga gatgaagatg
    19921 ttttcttaaa aaatcccaac gtttcgtctt ccatgaaaac aatgccgcgg catgcgcggc
    19981 attatgtagc caggttggca aattttagtg tcttcagcca tgtcttgcac gctggatatc
    20041 gcgtatccgc tgtttttctg cgcgagccat cagataccag gcgataaatc cgacaattcc
    20101 gaccgcaccg agaattaacg ttgcaagggc gttgatttcc ggattcactc ccatccgcac
    20161 gctggaaaag accagcatcg gtaacgtggt ggctcccggc ccggaaacaa agctggcgat
    20221 caccagatca tcaagcgaca aagtaaaagc cagtaaccag ccagaaatga tcgcgggcat
    20281 gatcatcggt agcgtaatga cgaaaaacac tttcagcggc gtcgcaccga gatccatcgc
    20341 tgcttcttct atcgagcgat ccagttcccg caatcttgaa gaaataacga ccgccacata
    20401 agccgtacaa aaagtgacat gcgccagcca gatggtgagc ataccgcggt ccgcaggcca
    20461 gccaatggca tgagcaagcg cgacgaataa taacaacagc gacaagcccg tgatgccatc
    20521 tggcatcacc agcggcgcgg tgatcataaa ggcaaaacca tttgatccgc gaaacctgcc
    20581 aaaacgcacc agcaccaccg ccgcaatagt cccgaggatc gccgccgccg ttgccgcaca
    20641 ggcagcaatt gtcaggctta aaccaaccgc actcatcatc gcgtcatcgc gcaataactc
    20701 accataccag cgcgttgacc agccggccca caccgtcacc agcttcgagc tgttaaacga
    20761 atagataacc agcatcagca ttggcgcgta gagaaaggta aagcccagca gcaaaatcac
    20821 aatccgccag ggcgaacgaa ctaccggtaa attattcatc cgtgttctcc cacgcttttt
    20881 tgctggtgtt tgtggaacca cattatcggc acaattagca gcagcaacat gatgatcgct
    20941 accgccgagg ccaccggcca gtcgcggtta ttgaaaaact cttgccataa cacgcgcccg
    21001 atcatgatgc tgtccgggcc accgagcagt tccgggatca caaactcgcc cacagccggg
    21061 ataaacacca gcatcgatcc ggcaataatc ccacctttag tcagcggcac gatcacagta
    21121 aagaacgttt tcagcggtcg tgcaccgaga tccagcgctg cttccaccag cgaataatca
    21181 atacgaatca acgcggtata aatcggcagt accataaacg gcacgtaagc gtaaacaatg
    21241 ccgatataaa cggccagatt ggtatgcaga atggtcagcg gttgatcgat aacccccagc
    21301 cacagcagaa aattattcag cacaccgttg ttttttaata ttcccatcca ggcataaacg
    21361 cggatcagaa acgaggtcca cgacggcagg atcaccagta gtaataaaat attacgggtc
    21421 gaaggcttac tgtgcgccac cgcccacgcc agcggatagc cgatcagtaa acagcaaaat
    21481 gtcgaaatcg ccgccacctg taacgactgg agataagcat caaaatagag cggatcgtcg
    21541 gtcagttgca gaaaattacc gagattaaga gtgatggaaa gttgcccgtc agcccactcc
    21601 atcagttcgg tataaggtgg aatagcgcgc gccatctctc ccaggcttat tttaaagaca
    21661 atcagaaatg gcagcagaaa cagcaagatc aaccagatat atagcaacgc aatgaccagt
    21721 ttgcgcccat gcttcatttg cagctgcgac aaccacagct taaatccgcc cggcggtttc
    21781 gactgggcag caggttcaag tgtactcatc tttgctcctt aaaccgtcag caccacacag
    21841 ctgtccactt cccagcacaa acgcacttcg tcgccccagg tcggtaaccc tttacgatga
    21901 cgatgggcgt tttgtagctg ggcgctgatc atctgcccac ttttcagtcg aacgtgatac
    21961 accgaaagat cgccgagata ggcaatgtgt atcacctccc ccaccgcgaa gttacaacca
    22021 ttggcgggcg gctcttcgca aagcatgatt ttttccgggc gcagcgctac gtgtaccggc
    22081 acgttatcga ccactgaagc atccgcatcg actttcagtg gatgcaccag ccccggcgaa
    22141 tcaagcacca ggccatcttc ctgacgctct ttgagtacac cttcaaagac attaaccgaa
    22201 ccgataaatt cagcgctgta gcgggtagtt ggatgctcgt agatctcttc cggctcgcca
    22261 atctggacaa atttcccgcg attcataatg gcgatacgcc ccgccatggt catcgcctct
    22321 tcctgatcgt gggtgaccat cacacaagtc acaccgacgc gctccagaat atccaccact
    22381 tcaagctgca tccggtcacg cagcttttta tccagcgcgc ccatcggctc atcgagcagt
    22441 aatagtttcg ggcgtttggc aaggcttcgg gccagggcca cacgctgtcg ctgaccaccg
    22501 gaaagctgat gcggtttgcg tttggcgaac tcctgcatgt gcaccaggcc gagcatctca
    22561 tggacccgac tggcaatttc cgctttcggt agtttgtcct gtttcaggcc aaaagcgatg
    22621 ttctgttcca cggtcatatg cggaaacagc gcgtaagact gaaacatcat attgatgggg
    22681 cgcaggtaag gcggcacctg tgacaaatcg acgccatcga gcattatctg cccggcagaa
    22741 ggttgttcaa aacctgccag catacgcagc agcgtggact tgccacagcc ggatgcgccc
    22801 agcagcgcga agatttcacc tttgtagatg gtcaggctga catcatcgac cgcatgctga
    22861 ccatcgtagg atttggtgag gttgcggatt tctaatagcg gcgtcagcgc cttacgggtt
    22921 ttcgcctgcg ggcgagggat agcgtcattc acggctgctc tccggcattg aatgtgtgca
    22981 agcgcaccat gatggtgcga attgccggct ggtgtggtgc gcccgcccgg catctaaggc
    23041 tgcggattat tttccgctct tcactttggt ccacgcgcgg gtgcgcacac ggtcgatttt
    23101 cggatcctgt actttcagag tgaatagctt cgcacgaacg tccgccggag gataaatacc
    23161 cgggttctca cggacttccg cactcaccag cggcgtggct gctttattgg cgttggcata
    23221 gaacacatgg tcggaaatat gcgctactac atccgggcgc agcaggtaat tcaggaactg
    23281 ataggcttcg tctttgtttt tggcatccgc aggcatggcg aatacatcaa agaacgccat
    23341 cgccccttct tttggaatcg agaacgagac attcactcca ttcttcgctt ccttcgcgcg
    23401 gtttgacgcc tgccagacat cacctgccca gccgatagcg acgcaaatat cgccgtttgc
    23461 caggtcgtta atgtattgag atgaatggaa ataacgaatg ttcgggcgca gctttaacag
    23521 cagatcggtt gccggtccgg tgtaatcatc cgctttagtg ctgttgggat ctttgccgag
    23581 ataattcaac acggtagcaa aaacttcttc tggcgcatcc aggaaagaga caccgcagct
    23641 tttcagtttt tccagatttt caggtttgag gatcaagtcc cagctatcga cgggcgcgtt
    23701 ttcgcccagc acagctttaa ctttatcaac gttatagcca atcccggtcg tcgcccacat
    23761 atagggcata gcaaatttat tgtcgggatc gtgtttggcg accagcttca gcagctccgg
    23821 gtcgagattc ttccactccg gcaatttgct tttgtccagc ggctggaaaa ctcccgcagt
    23881 caactggcgt tcaagaaagc tggcagatgg aaccaccaag tcaaagccgg tgctacccgc
    23941 cattaatttg ccttccagta cttcgttaga gtcgaaaaca tcgtagacga ctttaatacc
    24001 ggtttctttt tcaaaattgg ccaccgtgtc cggggcgata taatcagacc agttataaat
    24061 gtggagtgtt ttttgttcag ccgcgagcgt gccgacagag acggccatca gagcacccgc
    24121 agccagaccc gaaagccatt ttttatttaa ggcggtcata tctctttcct tctgaaagtt
    24181 cgttaacaaa cgaacaaaag ttgtcacact aaaagatatg caataaacgt gcatattttt
    24241 tatcggcggg gtgtaagaaa agagaactct ctcccggcca gcactgctcg ctatataaaa
    24301 gcatcgcaag aataacttta gccagggttt gagactatag ctcggattaa aaaacggtat
    24361 ttaacaaatt tttatgcgta tttgctctat gtgataaagc caaatcgaca caagagaagt
    24421 gaaataatct ttaagaaaag gttaaaaaca gcagataatt ttgcagcgcg cagccactgc
    24481 accagcgact gcgtgctcga aaggagttat cagtggagga aatagttttc ggtaacgttg
    24541 ttttgccctt catcgtcagt cggtaacagc agttgatggg cattagcttc aagaatgacc
    24601 atcgaaatct gctcttcgct ctgacgcacg aaccatgcaa actgctcata ggttacgccc
    24661 tgcataacgg ataaagactg gcaaaccacc agctttggca gattatcatc ctgcatatca
    24721 agaaatgctt tcacggtcag cgaactggca ttgatggcag ataaatccgc cgccagcggc
    24781 aatacggctg aaggtcggac ttccgccatg gcagaaaaca ggatcgtgtt atcgatcaga
    24841 tcgattttgg catcaaagac accgtcgaaa ttctgcatat ggggcagatg cagagcctga
    24901 cagttatcac attcaaaaaa actcatcccc aggtcatcga gccattgacg cagcgtatcc
    24961 agaccaggaa cgaccagcga tgtcataatt ttgtacaacc tctttcgata aaaagaccgg
    25021 cacagcttac gcaaaaagcg caggcaaaac catgatcagt aatgtgattg cgactaacca
    25081 cccgttttca ggcaatattc tgtcgtagcg tggcgttcga tccagcggat cattttaccc
    25141 gcgatgtcga taccagtggt tttttctatt ccttccagcc ccggcgacgc attcacttcc
    25201 atcaccaacg gcccgcgatt agcacgcaga atatccacac cagcaacgtc cagcgccatc
    25261 gttcgcgcgg cttttatcgc gatttcacgc tcctgtggtg tgatactggc gacgcttgcc
    25321 gcgccgccac gatgcaaatt ggaacgaaaa tcgccctctt tcgcccgccg ttcaatcgca
    25381 gcgaccactt catcgccaac aaccagacag cggatatcgc acccttgcgc ctctttgata
    25441 tattcctgca ccagaatatg cgcgttcaga ccgcggaaag cgtcaatcac gctttccgcc
    25501 gcctgacgcg tctccgccag cacgacgcca attccctgcg tgccttcaac caacttgacc
    25561 accagcggcg caccaccgac catgtcgatt aaatcgctgg tatcatccgg cgaatgcgca
    25621 atgcccgtga caggcaggtc gatgccctga cgcgccagca gttgcatgga acgcaatttg
    25681 tcacgcgccc gggcaatggc gaccgactca ttgagcggat agctccccag catctcgaac
    25741 tggcgcagtg ccgccgtccc ataaaaggta atggcggtgc caatacgcgg gatcactgcg
    25801 tcaaaatggg gtaacttgcg gcctttgtag tgaatagaag acgccgcagg atttatgttc
    25861 atgtagcaag aaagcggatc aagaatttca accaggtgac cgcgctgtat agcggcttca
    25921 cgcagccgct tacacgaata gagcgttcca tcccgggaca atatggcaat tttcaccctg
    25981 cacctctctg tcagacctgg tcaaaagccc gcgtatcata catcgacgtg gcggttttag
    26041 cgcgtcgccc aaccctgttt gtgcaaataa tccagaataa atgggcggct ttctttaatg
    26101 attgttcggc ggatatgatc gctccaggta tcccggcgat tattgctgcc acgggtgagg
    26161 taatattccg ccagttgctc gtcatactgc gccagtgcgt ctttatccag cggttgatag
    26221 ctgttttcat gcaccaaaat ggaggccggt aaacgcggct tgagatccgg attatccgca
    26281 ggccagccaa ggcacagccc aaacagcggc agaacatgct gcggtaattt cagcagtttc
    26341 gtcaccgctt caatattatt gcgcaggccg ccgatatata ccccg
//
LOCUS       DAJVRF010000047        25647 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJVRF010000047 DAJVRF010000000
VERSION     DAJVRF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.047"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(76..807)
                     /gene="rlmB"
                     /locus_tag="OQN13_001776"
     CDS             complement(76..807)
                     /gene="rlmB"
                     /locus_tag="OQN13_001776"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCS1615154.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(987..3428)
                     /gene="rnr"
                     /locus_tag="OQN13_001777"
     CDS             complement(987..3428)
                     /gene="rnr"
                     /locus_tag="OQN13_001777"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCS1615155.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYVPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="OQN13_001778"
     CDS             complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="OQN13_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCS1615156.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4097..5395)
                     /gene="purA"
                     /locus_tag="OQN13_001779"
     CDS             complement(4097..5395)
                     /gene="purA"
                     /locus_tag="OQN13_001779"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCS1615157.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5499..5696)
                     /locus_tag="OQN13_001780"
     CDS             complement(5499..5696)
                     /locus_tag="OQN13_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCS1615158.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="OQN13_001781"
     CDS             complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="OQN13_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCS1615159.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="OQN13_001782"
     CDS             complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="OQN13_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCS1615160.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="OQN13_001783"
     CDS             complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="OQN13_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCS1615161.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="OQN13_001784"
     CDS             complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="OQN13_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCS1615162.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="OQN13_001785"
     CDS             complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="OQN13_001785"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCS1615163.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="OQN13_001786"
     CDS             complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="OQN13_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCS1615164.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="OQN13_001787"
     CDS             complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="OQN13_001787"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCS1615165.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="OQN13_001788"
     CDS             complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="OQN13_001788"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCS1615166.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="OQN13_001789"
     CDS             complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="OQN13_001789"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCS1615167.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16016..17155
                     /gene="queG"
                     /locus_tag="OQN13_001790"
     CDS             16016..17155
                     /gene="queG"
                     /locus_tag="OQN13_001790"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCS1615168.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17138..17191)
                     /locus_tag="OQN13_001791"
     CDS             complement(17138..17191)
                     /locus_tag="OQN13_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1615169.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17425..17500)
                     /locus_tag="OQN13_001792"
     tRNA            complement(17425..17500)
                     /locus_tag="OQN13_001792"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17465..17467),aa:Gly,seq:gcc)
     gene            complement(17536..17611)
                     /locus_tag="OQN13_001793"
     tRNA            complement(17536..17611)
                     /locus_tag="OQN13_001793"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17576..17578),aa:Gly,seq:gcc)
     gene            complement(17769..17844)
                     /locus_tag="OQN13_001794"
     tRNA            complement(17769..17844)
                     /locus_tag="OQN13_001794"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17809..17811),aa:Gly,seq:gcc)
     gene            complement(18055..18600)
                     /gene="orn"
                     /locus_tag="OQN13_001795"
     CDS             complement(18055..18600)
                     /gene="orn"
                     /locus_tag="OQN13_001795"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCS1615170.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18695..19747
                     /gene="rsgA"
                     /locus_tag="OQN13_001796"
     CDS             18695..19747
                     /gene="rsgA"
                     /locus_tag="OQN13_001796"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCS1615171.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19844..20812
                     /gene="psd"
                     /locus_tag="OQN13_001797"
     CDS             19844..20812
                     /gene="psd"
                     /locus_tag="OQN13_001797"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCS1615172.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20834..24157
                     /gene="mscM"
                     /locus_tag="OQN13_001798"
     CDS             20834..24157
                     /gene="mscM"
                     /locus_tag="OQN13_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCS1615173.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24308..>25647)
                     /gene="yjeM"
                     /locus_tag="OQN13_001799"
     CDS             complement(24308..>25647)
                     /gene="yjeM"
                     /locus_tag="OQN13_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCS1615174.1"
                     /translation="MMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWM
                     VSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIA
                     RITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVF
                     AIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLS
                     NGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTL
                     CYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAF
                     FNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVTF
                     ANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5934 a         7194 c         6499 g         6020 t
ORIGIN      
        1 tcaccgtaat tatcttacca gctatagaca aaaaaaacca tccaaatctg gatggctttt
       61 cataattcag acgaactagc tgcgctgacg aaccgcttca aataagcaaa ttccggttgc
      121 aaccgaaacg ttcagggaag aaacacttcc tgccattggg atgctgatca actcatcgca
      181 atgttcacgg gtcaggcgac gcataccttc accttccgcg cccatcacca gcgccaagcg
      241 tccggtcatt ttgctctgat agagtgtatg atccgcctcg cctgccgtac cgacgatcca
      301 gatattctct tcctgcaaca tacgcatggt gcgcgcaagg ttagtcaccc gaatcagtgg
      361 aacgctttct gccgcgccgc aggctacttt tttcgccgta gcgttgagct gtgcggagcg
      421 atctttcggc acaatcaccg cgtgaacgcc agcagcgtcc gcgctacgca ggcacgcgcc
      481 gaggttgtgc ggatcggtta caccgtcgag gatcagcagg aacggttgat cgagcgaagc
      541 gatcagatcc ggcagatcgt tttcctgata ctgacgtcct ggcttcacgc gggcgataat
      601 gccctgatgc acggcaccgt cgcttttctc gtcgagatat tggcggtttg ccaactggat
      661 aaccacgccc tgagactcaa gggcgtgaat cagcggtaac agacgtttat cttcacggcc
      721 ttttaaaata aagacttcct gaaaacgttc aggggcgcgc tccagcaggg cctgcactgc
      781 gtggatgccg taaatcattt cgctcattaa tgtactcgtt gtttacgttt tgcctgatgc
      841 actacgttta tccggcctac atgatctctg caatatattg aatttgcgtg attatgtagg
      901 ccggataagg cgttcacgcc gcatccggca tgaacaaagc gcattttgtc agcaatctaa
      961 ccctcttctt ttaaagaggg tattgatcac tctgccactt ttttcttcgc cgcacgcttc
     1021 gctttggtcg ctgcggctat tttctgcgtt ttcgccgatg gctttttcgc ttttctcgcg
     1081 tctttcttcg ccgctttcgg cttcgttttt ttctcaccgc ggaaggcgct gtctggctca
     1141 aagtttacct ttttaccgac ctgacgacgc ttgccgcctt ttttgcctgc atcgcctttt
     1201 ttcgctttct cgcgcgccgt tttaccgacg ttgcgcggtg cgcgttcact ggagatcaga
     1261 ctaaagtcga ttttgcgctc gtccatatta accgcttcga cgcgaacttc cacgcgatcg
     1321 cccaggcgat aagtctggcc gctggattcc cccatcaggc gttgccctac ctggtcaaag
     1381 cgatagtagt cattgtccag cgaagagaca tggaccagac catcaatgaa caagtcgtcc
     1441 agacggacga agaagccaaa gccagtgacg ctggaaatta cgcctttaaa gacgttacct
     1501 acctggtcga gcatgaagtc acacttcagc cagtcagcga catcgcgcgt tgcttcgtcg
     1561 gcacgacgtt ccgccatcga acagtgctga cccagttgca acatctcttc catcgaataa
     1621 tggtagccgc cggtttcagt ggtgttgccc tgatgcccct gctctttcgc cagcagatac
     1681 ttaatggcgc ggtgcagcgt caggtcagga taacgacgaa tcggcgaagt aaagtgcgca
     1741 taggactgca atgccaggcc aaagtgacca cggttttctg gatcgtaaat cgcctgtttc
     1801 atcgagcgca gcagcatggt ttgcagcatt tctgcatcag gacggtcggc aaccgactcc
     1861 agcagttccg cgtagtcacg cggttccggc ttgttaccac ccggcagctc cagccccagc
     1921 tccgccagca ctgaacggaa agaggtaatc gcttcggtgc tcggcttgtc gtgaatacgg
     1981 aacagtgccg gttctttcgc tttctcaacg aaacgcgccg ccgagatatt cgccagaatc
     2041 atgcactctt caattaattt gtgcgcgtcg ttacgctggg tctgttcgat acgttcaata
     2101 cggcgttcag cgttgaaaat gaacttcgct tcttcgctct caaatgagat cccaccgcgt
     2161 tcttcacggg ctttatccag cactttatag aggttatgca actcttcgag atgcttaacc
     2221 agcgggacgt actgctcgcg cagatcctga tcgccctgca gaatatgcca gactttggtg
     2281 taggtcagac gcgcgtgaga gctcatcacc gcttcgtaga atttgtagcc cgtcaggcgg
     2341 cctttcgacg aaaccgtcat ctcgcacacc atacacaggc ggtctacctg cgggttgagc
     2401 gaacacaggc cgttagagag cacttccggc agcatcggga taacctgcga agggaagtac
     2461 accgacgtgc cacggttacg cgcttctctg tccagcggcg ttggcggacg cacatagtag
     2521 ctgacgtcgg caatcgcgac ccataaacgc cagccgccgc cgcgtttttt ctcgcagtaa
     2581 actgcatcgt caaagtcacg ggcgtcttcg ccatcaatgg tgaccagcgg taaatcgcgc
     2641 aaatcgacac ggcccgcttt tgcttcttcc ggcacttctt ctttcagccc cgcaacctgt
     2701 tgctcaacag cctgcggcca gatatacgga atttcatggg tacgcagagc gatatcaacc
     2761 gccatgccgg tgcccatatt gtcgcccagc acttcgacga ttttacccac cgctttggtg
     2821 cggcgagtcg gacgctgagt cagttcgact acgaccacaa agcccatccg cgcgcccatg
     2881 atctgatcgg gcgggattaa gatatcgaag ctcagacggc tatcgtcagg aaccacaaag
     2941 ccgacgcccg cttcggtaaa gtagcgacca acaatctggc tggtttttgg caccagtacg
     3001 cggacaatac gcgcttcacg acgaccttta cggtcagcac ccagcggctg cgccagcacc
     3061 tgatcgccat gaatacaggt tttcatctgc tcgctggaga gatacaaatc atctttacgc
     3121 ccttcaaccc gcagaaagcc gtagccatca cggtggccaa taacggtacc tttcaccagg
     3181 tcgaggcgtt ccggcagcgc atagcactga cgacgagtga agaccagttg accatcgcgc
     3241 tccatcgcgc gcaggcggcg acgcaggcct tcaagctgct cttcgccttc aatgtgcagt
     3301 tctaccgcca gctcatcacg gctggccggt ttttcacgtt tggttaaatg ttcgaggata
     3361 aattcccgac tagggatggg attcgcgtat ttttcagctt cgcgttcctg gaaaggatct
     3421 tgtgacatct cggttcctcc gttgtcatct ctgatgaaga ttttcgtcac tccaccagca
     3481 ataatttata aagcggttga ttctcttcaa ccaaatcggc aagcgtgtag ttatccagtt
     3541 ccgtaagaaa actttgcacg gccttagaaa gcgcctgttt caaccgacag gcaggtgtaa
     3601 tgtggcaaaa ctcactgctg caattcacca gcgataaggg ctccagctcg cgcaccacat
     3661 caccaatacg tatcgcactc gccggtttac ccaggcgaat gccgccattt tttccacgaa
     3721 cagcagtcac gtagccggca cgactaagtt gattgattat tttgaccata tgattacggg
     3781 agacgccgta gacgtcagtc acttcagaaa tactggtcat ccgcccttct ggcaatgacg
     3841 ccatgtagat cagcgcacgt aatccgtaat cagtgaaact cgttaactgc acatcaacct
     3901 caaaagggaa atcgggaaaa aatacattta tattgatgat aaaccagcca caagctgtgt
     3961 cgctaattta tttcagttcg gggaaggaaa aaagcgagga tttaacactg tgtcggatag
     4021 cgtaatggct taaccgacac agcaatgtat cacactgtta gcccggcagg caaaatatct
     4081 gccaggcgta ccagaattac gcgtcgaacg gatcgcgcag aatcatggtt tcagtacgat
     4141 ccggaccggt agagatgata tcgatcggca caccggtcag ctcttcaata cgcttgatgt
     4201 agttcagcgc cgcctgcggc aggccgctac gatctttcac gccgaaagtg gattcagacc
     4261 agcccggcat ggtttcgtaa atcggctcta cacctttcca gtcgtcagct gccagcggag
     4321 tggtagccac ttcgcgacca tccggcatac ggtaagccac gcagagttta acctctttca
     4381 ggccatccag aacgtccagc ttggtcaggc aaaagccaga cagggagttc agctgtaccg
     4441 cacgacgaac ggcaacggtg tccagccagc cggtacgacg acgacgaccc gtagttgcgc
     4501 cgaattcgtt accctgcttg cagaggaact cgccagtttc atcaaacagt tcggtcggga
     4561 acggacctgc acctacacga gttgagtaag ctttgaggat acccagaaca taatcaacat
     4621 aacgcgggcc caggccggaa ccggtcgcca cgccaccagc agtggtgttg gaagaagtta
     4681 cgtacggata agtaccgtgg tcgatatcca gcagcgtacc ctgcgcacct tcaaacatga
     4741 cgaaatcgcc acgctgacgc gcctggtcga gcaggtcaga aacgtcaacc accatagaag
     4801 tcaggatgtc ggcaacagcc atcgtatcat ccagaacttt ctggtaatca accgcttcag
     4861 ctttgtagta gttaaccaac tggaagttgt gatattccat cacttctttc agtttttcag
     4921 cgaaggtttc tttgtcgaaa aggtcgccaa cacgcagacc gcgacgtgct actttatctt
     4981 cataagcagg cccgatacca cgaccggtgg tgccgatcgc tttcgcgcca cgcgctttct
     5041 cacgcgcgtt atccagcgca acgtgataat caaggatcag cggacatgct tcagacagca
     5101 gcagacgctc acgaacgggg atgccacggt cttccagttc tttcatctct ttcatcagcg
     5161 cggccggaga cagcacaaca ccgttaccga tgatgctggt tacattctcg cggagaatac
     5221 ctgatggaat aagatggaga acggtttttt caccgttgat tacgagagta tggcctgcgt
     5281 tgtgaccgcc ctggtagcgt acaacatatt tagcccgttc agtcagaaga tcgacgatct
     5341 tacctttacc ttcgtcaccc cattgggtgc ccagtacgac gacgttgtta cccatttttc
     5401 aaaatcaccg tttgcttaaa aatggattct accatcgctt tttcagagtt acagcacttt
     5461 ttgcactcaa aaatgacctc gatcagtctg ttttttgttc agccaatcgt tttcctcaac
     5521 atgtagtaga ccacaacgcc cgcaaccaca agtccaccgc caaaacgacg taaaatatta
     5581 tcgggcaaat tggtcatcgc agagatcatc ttcttccatg ccttcgggta aagcatcggc
     5641 cctaaacctt ccagtaccaa aaccagggca agcgccagcc agattgtcga attcattcat
     5701 tatccttata gaaaaagaaa accaccgaca tccctgagga tgcggtggct ttattgacct
     5761 gtaccgcagt cgttatatta acgcgttgcg gaagtcggcg tcttcatgta gcggaagaaa
     5821 tcgctatccg ggctcatgac catcacgtcc tgattgccag agaagctgtt ctcataagca
     5881 cgcaggctac ggatgaatgc gtagaagtcc ggatctttac tgaatgcatc agcaaacagt
     5941 ttggctgctt cggcatcgcc ttcaccacgc atgatgcggc cctgacgctc agcttctgcc
     6001 agcgttctgg tcacttcata gtcggcagtc gcgcgcagtt tttccgcttc ttcctgacct
     6061 tgtgaacggt gacgacgcgc taccgcttca cgctcggcgc gcatacggtt gtagatcgct
     6121 tcagacactt cggtcggcag gttgatctgc ttgatacgca catcgacaac ttcaataccc
     6181 agcgccgcca tactgttcgg gttgatgacc ggaactttgc ccttcgtctc agccgttacg
     6241 cgctctgccg cttcggcaat ggcgttatct gccgccgggg tagtaacttc atcttctgta
     6301 cccgcagaac cggagttcag cgcgtcacgt acttcgaggg tcagacgacc acgggaatcg
     6361 gtgacgatgt ctttcacgtc caggcgacca atttcagaac gcagacggtc agagaactta
     6421 cgtttcaaca gcacttccgc ttgcgaaatg tcgccaccac ccgttgccag gtagtaacgg
     6481 ctgaaatcgc tgatgcgcca tttgatgtaa gaatcgacga tcaggtcttt cttctctttg
     6541 gtcacaaagc ggtcggcctg gttgtccatg gtctgaatac gtgcgtcgag cattttcacc
     6601 gtttcaatga acggtatctt gaaatgcaga cccggctcat aaaccagagg tttgttgtca
     6661 tcgtcacgca gtaccttacc aaaacgcagc gtaataccgc gctcaccttc tttgacgaca
     6721 aagacagaca tgtaaagcac taccagcacg atgatgataa tcgcgataac tgacttacgc
     6781 atcgttattc cccctgacgc tggtagtcgt tacgctgcgc gttggcgcgg cgttggtcca
     6841 taatatcgcc ctgactggtg gacgacgtgt tgcttgctcc actggttgtg gaggaagagg
     6901 ctggcggcag acgcagcaga ttgctggcac cgttatcgct cttcgccgca ggggcgttac
     6961 cgcctttcag catctggtct aacggcagaa ccatcaggtt gccaccttta tcgttaacca
     7021 gcactttgcg ggtgttaccc aacacttttt ccatcgtctc gatatacaga cgctcgcgag
     7081 taatttccgg cgcggcttta tattccggca gaagtttagc aaagcgcgcc acttcaccct
     7141 gagcttccag gatggtctgg gccttgtacg cacgcgcctc ttcgaggata cgttgcgcct
     7201 gaccgttcgc acgcggctga acttcgttgg tatacgcttc tgcttcacga atgtattgct
     7261 gttcgttttc acgcgcggca atcgcatcgt caaacgccgc ttttacttct tccggcggac
     7321 gagctgcctg gaagttgacg tccagcagag tgatacccat gtcatacgga cggatagtct
     7381 cttccagttc gcgctgagta tcgctacgaa tcacggtacg accttccgtc agaatgcggt
     7441 ccatggtgta tttaccgata actccacgca gggcgctgtc ggtagcctga cgcaggctgt
     7501 catccgggct ggtcacgcta tacagatatt tttccggatt ggtgacgcgg tactgcacgt
     7561 tcatctcaac gcgcactacg ttctcgtccg acgtcagcat cacaccagaa gcggccagtt
     7621 cacgcacggc ttccacgttc accggtttga cttcgtcgat aaacgtcggt ttccagttca
     7681 gacccggctc aaccagatgg ctgaatttac caaagcgtgt taccacgccg cgttcggctt
     7741 ctttaatggt atagaaacca ctggccgccc agataatgac aatcgctgcc gctgcgatgg
     7801 taacgacacg accgccaagc tgcgggcgcg ggccttgcga tgaactgcca ccgccagatc
     7861 cggtgccttt accgccgccc agaccaccga gctttttgct cagtttgcgg aagatatcat
     7921 ctaaatcagg tggcccttga tcgcgaccgc ctttgtttcc atttccctca gagttgccgc
     7981 caggtttgct gcttccccac gggtcgcggt cttgtccgtt attaccgggc tgattccacg
     8041 ccatgtttgt gctccatatt tgttatgcgg tgatccctgt tgtctttcga cgccagagga
     8101 tatgactcca cgcttcagac gctacgccgt tagatcaggt aatcgatcaa cgccggttct
     8161 tgtttacaga ggcgacgcca gtcaacgatc ggcatacgaa cttgcagact tacgctgccg
     8221 tcctcctcca tccactcttt ttctattgcc tgaagctgat aaaaacgact tctcagacgt
     8281 ccttcctgcg gcggcaaacg caatgtatgc tgcgccacct cgccggaaag ccgctccgtc
     8341 aaagcctgaa aaagctgtgg tatccccgct ccggtctgtg cggaaagcca gacacggatc
     8401 ggtttgttct cttcgtcccg atcaatacgc ggttcgaaat cttccagcat atcgatcttg
     8461 ttcatcacca gcagggttgg gatctcgtga gcgtcgatct cttcaagaac cgtattcacc
     8521 gcttcgatgt tttcttgtac acgtacatcc gccgcatcaa tgacgtgcag cagtaatgtg
     8581 gcttgccgcg tctcttgtaa cgtggcttta aatgctgcca ccagatcgtg cggcaggtgg
     8641 cgaataaacc ctacggtatc tgcaagtacg gtttcaccga catccgcgac gtcaatacgc
     8701 cgcaacgtcg ggtcgagggt ggcaaacaac tggtctgccg cgtagacccg cgcttcggtg
     8761 atgcgattga aaagggtaga tttaccggcg ttggtatatc ccaccagcga aacagtagga
     8821 acgtcggctt tgatacgcga ttgccgcccc tgctcacgct gcttttcaac tctttctagg
     8881 cgcgactgta tctgcacgat gcgattacgc aacaaacgac ggtcggtttc gagctgggtt
     8941 tcacccggac cacgcaaacc tatcccgcct ttctgtcttt caaggtgggt ccagccacgc
     9001 accaggcgcg tagccagatg gcgcagctgc gctagctcaa cctgcaactt accctcatgg
     9061 gtacgcgcac gttgggcgaa aatatctaaa ataaggccgg tgcggtcgat aacacgacac
     9121 tcgcacaaac gctccaggtt acgctcttgc gccgggctca gggcatggtc aaaaagaacg
     9181 accgaagcac ccgtcgcttt gacagcttcc gcaatttcaa ctgctttacc ttcacctaca
     9241 aaatactttg ggtgcggcgc tttacggcta ccggtaatca cctgcaatgc ttcgacaccg
     9301 gcggaagaga ccagagattc aaactcctgg aggtcttcca tatctttgtc ttgcgtaaaa
     9361 tagatgtgta ccagtaccgc ctgctcacca gcatcataac ggtcaaacaa gcgtataacc
     9421 ctctaaatag atcagcgggg aacgcaggat cgctggctcc ccgtgtaaaa aaaacagccc
     9481 gaaaccttat tcggtttctt cgctgtcctg ttgcgcggaa gtattctgcg cgctgctacc
     9541 atgatggtag ttactgctgg taccgccacc ggcgttgtta ctgtgatgag aaaccgggcg
     9601 agacgggaca acagtagaaa tcgcgtgctt gtaaaccatc tggctgaccg tgtttttcaa
     9661 caggatcacg aactgatcaa aagactcgat ttgcccttgc agcttaatac cattcaccaa
     9721 ataaatagaa actggaacac gttcccgacg cagtgcgttc aggaacggat cttgtaaaga
     9781 ttgcccctta gccattctct cttttcctta tatgcttatt tgtactttga acctttcgat
     9841 tctgaaaaaa ttgcgcacga tacgtctcaa ttgtacacat tcagcctgcg atagcaccaa
     9901 caacctgtaa tacttcgtca cgcgcctgtt ctggtttttc actgtcaagc cagtgaaccc
     9961 cttcccaacc acgcagccag gttatctgcc gcttcgccaa ctgtctcgtg gcgcaaacac
    10021 ctcgataaac catttcatcg tatgagattt cgccttcaag gtaagaccac atctggcgat
    10081 aacccacgca acgaatggaa ggcaagtccg tatgcaaatc tcctcgggca aaaagcgccc
    10141 ggacttctgc ttcaaaacct gaagccaaca tctgatgaaa acgctgctca atgcgttgat
    10201 ggagcagttc acggctcgcc ggggcgatgg cgaactgatg cacctgatac ggtagagcgt
    10261 ctcctgacgt ttgcgtcagt tccgttaaag ttttacccga aatgaaaaaa acttccagtg
    10321 cccgggaaag cctttgtgga tcatttggat gaatccttgc tgccgcaacc ggatctacct
    10381 cctgaagttg acgatgcaat gactcccaac cttgctctgc cgcctgttgc tcaattctgg
    10441 cccgcacttc cgggtctgcc gacggtagcg gcgacaaccc ttccagcaat gccttgaaat
    10501 acaacatcgt accgcccact aacagtggga tccgccccgc cgcggtgata tcggccattt
    10561 ccgccagcgc atcgcggcga aaatcagcag ccgagtaagc ctgcgacgga tcgcgaatat
    10621 ccagcaatcg gtgcggcgcg gcgagtaact cttcagcgtt cggcttcgcc gtcccgatat
    10681 ccatcccttt gtaaataagg gcagaatcaa cgcttatcaa ctctactggt aaaattttac
    10741 gcagctcaat ggctaacgcc gttttaccgg aggccgtcgg ccccatcaaa aaaatcgcct
    10801 taggcaggct cgccttactg atatcactca tctttcaggg cttttatcgc cggatgtaaa
    10861 tcaacagatt gtaacagacc acccggcggc gttttcacaa gttgcggaca taaccgttcc
    10921 acgtccgcca gcagggttat ggcctgtgcc attgaccact gcgcatgttc gctcatcaga
    10981 tttcgtgcaa tccactgcgc aatattgcca ggttcgaata cggactgctt cgccaggtag
    11041 cctatcagtt caggaatcaa gatttgtaaa ttttgttggc gtaagggtaa aggcacggcc
    11101 ctgatggtca catgctgtgc atctgactgg aaatcaatac ccaattccgc cagggcagac
    11161 tgcgcttttt ctaatgccga tttttcttcg ccagaaactt ttagccgcaa cggaataagc
    11221 agcggctgag cgcaaactgg cgcttcaccc ggcgtcagtt gtacctgacg cagccaacgt
    11281 tctgccactg gcaaggctaa aagtgaaatg ttgccgtcgc gctccagcaa cgcacagtcg
    11341 gaatggacga tagtcagtac ccgaccaaaa ctctgactgt tcgccgcaag tgcaggttcc
    11401 tgcggttccg gcgcttttaa tttttgcatc ggcgcgggcg tttgcaaaag ctggcgatac
    11461 acttcacctt gctgtttctg gtagcctggc tgcgcattcg gccagggggc agccggacga
    11521 ctgcctgatg ctggcgcagg agtgtagcgc ggagctaccg gctcacgagc tgccggttct
    11581 gcaaagtgat tgcgccccgc cgccacgcgg ttttccggaa tggaacgcgg tgcaggttgg
    11641 ggttcatcgt ccagcggtag cggcgtttcc agttgctgtt gtagcacgct cagcacgccc
    11701 tgatagataa aatcatgcac cagacgcgac tgatggaaac gcacttcgtg tttggcgggg
    11761 tgcacgttga cgtccacctg atgcggatcg atctccagat acagcacaaa tgccggttgc
    11821 tgatcggccc ccagtttgtc ttcgcaggcc tggcggatcg cgtgattgat caggcgatcg
    11881 cgcatcatgc gaccgttcac gtagcaatac tgaatttctg ccagtgcggg cgtggtgtga
    11941 tttggatcgg ccacccagcc gcgtagcgtg agatcgccgt gttgccattc aatcgccagc
    12001 gcttgttcaa gaaaagcggt gccgcaaatc gcgcctaagc gccgttcttt ttgcccgcct
    12061 tccggcactg cgcggtactg acgcacaatt ttaccgttat gcgacaggtt gatcgtgacg
    12121 tcgaaacgcg ccagcgcaat gcggcggatg atctcatcaa tgtggttaaa ttcggttttc
    12181 tcggtgcgca ggaatttgcg ccgcgccggg gtgttgtaga acagatccag cacctccagc
    12241 gtcgtcccca caggatgcgc cgccggtttt accgtcacgt tcatatcgcg cccttcggca
    12301 taggcctgcc aggcttcctg ctgttctgcg gtgcgtgaag tgagcgtcag gcgggaaacc
    12361 gaactgatac tcgccagcgc ctcaccgcga aagcccaggc tgataatggc ttcgagatcg
    12421 tccagagagg cgattttact ggtggcatga cgagccagcg ccagcgccag ctcatctttt
    12481 ttgataccgc agccgttatc acgaatgcgg ataagtttcg ccccaccgcg ttcgatatca
    12541 atatcgatac gcgtcgcacc tgcatcgagg ctgttttcca ccagttcttt gactaccgac
    12601 gcaggtcgct cgaccacctc acctgcggca atctggttcg ccagttgtgg cggtaagacc
    12661 tgaattggca tcaatcgtcc ttagtttggc agcgtgcgat ctggcgtcgt caccgtactg
    12721 gcagtttgtg ccgttgcccc ctgcggcgca gattgcatcg gatgcgcaag gaaataattg
    12781 cgcaggcctt tgtaaatggc ttctgccagc tgttgttggt aatcgtcgct cgccagcaaa
    12841 cgttcttcgc tgttgttgct gataaaaccg gtttcgacca gtactgacgg gatatccggc
    12901 gaacgcagaa cgccgagact cgcgtgttct ggtcggcgtt tatgtatttc gccaatgcgt
    12961 tgcaactgac tgatcatact ggtcgctaca tcatacccta cccgctggga atgaccgaac
    13021 tgtaaatcca gcaccgcctg gcttaaatag gggtcagact gactgttcgc cagcacatcg
    13081 cccgccccac ccagcagctc cgactgtttc tcgtgctgct ccagccagct ggccatttcg
    13141 ctgttggcgc gacggttaga gagcacccat acggaagcgc cagtcgcact gcggttcggt
    13201 gcggcatcag cgtgaatcga cacgaggaaa ttggcgtttt gcttacgtgc cacatcgctg
    13261 cgccccatca ccgagataaa gtaatccccg tcacgggtta aaacgccttt aaacatcgga
    13321 tcgtcattga gcaaagtgcg caatttacgc gcgatggcga tggtgacatt tttctcccgc
    13381 gtaccaccgg ggccgatagc gccagggtcc tgaccgccgt gtccggcatc aatagcgatg
    13441 ataattttat cgccagtgtt agccgtcgcg cgcgctgccg gacgcgttac cgtattactg
    13501 ctgataacac ccgtagtgcg gttactttcc gttttaaacg gattgcgcgc cggttcgctg
    13561 acgcgcggtg cgacaaccgc aggcgtttca acgcgtttcg caaccacggg cggtggagga
    13621 ggcggtggcg gcacatcggc gttaatcgta aagacgacag tgtaattgct gccattctgc
    13681 cgcttcaccg cttcggtttt accgttttcg gtaagatcga ccaccagccg tagcgtttgt
    13741 gcatctttag gcgttccaga gcgaatcgcc ttcaccagat tattgccgct gaacaacaac
    13801 ggcagtccct gaatcacgcc cgtttgtttg atatcgagcg ccacggtgcg tttgctttga
    13861 tggctaaacg cataatcagg atcgccaata aaactcaacg ttatccgcgc ctgttgatta
    13921 ccgttagaaa cctgaatatc agagagcgtc gcggcaccca ccggcgtgca cagcagcagc
    13981 agcgtcgcta ccaaccaatt tctgatgcga tacatcatcc cgccaccttt caaaggttaa
    14041 ccggctaaac gcgccagcaa caattcaccc gcagaggaaa ccgcactcac gcgcgcctca
    14101 cggccttgtg cctgataatc aatgtgtatt tcgacatccg ggtcaggaag aacacctgta
    14161 ccttgttgtg gccactccac caggcagatg gcatcgttgg caaaataatc gcggatcccc
    14221 ataaactcca gctcctcggg atcggcaagg cggtacaaat caaagtgata aaccattaaa
    14281 ttgtcgagcg tatagggttc gaccagcgta taagtggggc ttttgacatt accctgatga
    14341 cccagagctt gtaaaaaacc ccggctaaag gtggttttac ctgcgcctaa atcgccatac
    14401 agatagatta cggttgcgcc atcgcaggct ttcgctaccc gctcgcccag gtctaatgtt
    14461 gcctgctcat cagggagcgg aattactcga ttcatcatgg tttttatcag tcacttccgg
    14521 gttaacaata cgctgtagcg tggaaaagag atcggttgcc agcatcccgc gcgttccaaa
    14581 acgcgccgcc agtacgtcag ctgccgcacc gtgcgcgaca cagcccgcac aggcggcatc
    14641 atacggcgac agtttttgcc caagcaatgc gccaataata ccagagagca catcgcccat
    14701 gccgccgctc gccatgcctg catttccggc atcaataatg cctaaagcgt caggatgggc
    14761 ggcgaccacg gttccggcac ctttcagcac cgctacgccg ccataacgtt gtaccagacg
    14821 tttggcgcaa tgtaagcggt cactttcaat ttcagcgacg gaacagccta acaaccgtgc
    14881 ggcctcgcca ggatgcggcg tgatcacgcg attgtgacgc ttatcgggat tgattgccag
    14941 caggttcaat gcatcggcat cccacaacat cggtttgcga aaattctcaa ctttttgcag
    15001 tgcttttttc ccccactctt gctggcccag accgggacca atcaccacca catcggccca
    15061 ttccaggctt tcggtaagag agtccatcgt cagttcatgc accatcaatt ccggtcgtgc
    15121 agtcagcagc ggcgcaatgt tttcactgcg ggtcagtact cggactaaac cagcaccagc
    15181 acgcagcgcc gcttcccccg tcatacgaat agcccccgcc gtgccgtgat cgccaccgat
    15241 aatcaccagc cgcccgtgat cgcctttatg cgaagtcggg cgacgcggtt tcagccagtg
    15301 agaaagttgt tctgccgaaa accgctgaat tttcgtctcc tgacctgcca gccagctatc
    15361 cagccccagt gagtcaaaat gcagttgtcc tgtaacatcc cgcgcttttc cagtaagcaa
    15421 gcctggtttc agcgcaataa aagtgatggt gtgatcggcg ttgatcactg cgcctggcgt
    15481 agcgccggtt tcagccagca ggccggaagg gatatcaacc gccacaatcg gcgcaggatg
    15541 ggaattagcg tggtcgatta actggctaat ggattcgcgg ggcgcttgct gcaagccggt
    15601 accgagcagc gcatcaacaa tcagatctac cgattcgggc cagacaatat tcgaagcatg
    15661 gatctcgcca cccgcgttta accatgcttc gcgtgccagc gcggcctctt ccggcaacgg
    15721 tttgtcgctc tcctgggcca acaacgtgac ctcaatgccg accgctttgg ccagtcgcgc
    15781 gaccacgtag ccatcgccgc cgttattacc atgaccgcac agcaccagcc agtggcgggc
    15841 gtcaggatac gccgaacgac acacctggaa tgcggcctcg ccagcgcgaa gcatcagctc
    15901 atagagtgtg agccccagca catctgccgc ctcgcgttct ccgcggcgga tatcgtcggc
    15961 gtaccagacg gtgtgtggta tacttacggg gtttttcttc attgtatggt ccgtcatgtc
    16021 agagcccctc gatctcaatc agttagcgca aaaaattaaa cagtgggggc tggaactggg
    16081 ctttcagcag gtaggtatta ccgataccga tctcagcgag tccgagccca aactgcaagc
    16141 atggctggac aaacaatacc acggcgaaat ggactggatg gcacgtcacg gtatgctgcg
    16201 cgctcgcccc catgagttat taccaggtac gctgcgtgta atcagcgtgc ggatgaatta
    16261 ccttcctgct aacgccgcat ttgccagcac gctgaaaaac cccaaactcg gctatgttag
    16321 ccgttatgcg ctgggccgtg actatcacaa acttctgcgc aaccgactca aaaagctggg
    16381 cgagatgatt cagcaacatt gtgtttcgct gaattttaga ccgtttgtcg attctgcgcc
    16441 tattctcgag cgcccgttag ctgaaaaagc tgggctcggc tggacaggta agcactcact
    16501 tatcctcaat cgcgaggccg gttctttctt ctttttaggc gaattgctgg tcgatattcc
    16561 gctgcccgtg gatcaaccag tcgaggaagg atgcggtaaa tgcatagcct gtatgacgat
    16621 ttgcccgacc ggtgccatcg tcgagccata taccgtcgat gctcgccgct gtatctctta
    16681 tctcaccatc gaacttgaag gggcgatccc ggaagagttg cgaccgttaa tgggaaaccg
    16741 tatttacggt tgcgatgact gccagcttat ctgcccgtgg aatcgctatt cgcaactcac
    16801 tacagaagac gatttcagcc cgcgtaagcc gctacacgca caggaactca ttgagttatt
    16861 tgcctggagc gaagagaagt ttttaaaagt cacggaagga tcggcgattc gccgtatagg
    16921 tcacctgcgc tggctgcgta atatcgccgt ggcattaggc aatgcgccct gggatgaaac
    16981 gattctggcg gcgctggaga gtcgcaaagg tgagcaccca cttcttgatg agcacatagc
    17041 gtgggcgatt gcgcagcaaa tcgagagacg aaatgcgtgc atagtcgaag tgcaattgcc
    17101 gaaaaaacag cgtctggttc gggtgattga aaaagggtta ccgcgtgacg cctgattcat
    17161 ccacagcttg tgaataaaaa taaaaacgca ttgcaattca agacgcataa aatcgtcaag
    17221 tgatcgactc gacaaaacgg aatcttgatt tttaacttat ttatcaatag gttaaaaaaa
    17281 taccattcac cgtgcgaagt ttctttgaaa ttaagattac agcctgagga tgtggataac
    17341 tctgtttaca agagtttgtc agagacaaca aaaaccagca ataacgcatc gcgtcgctgt
    17401 ggatatttta ttgagagaag aatttggagc gggaaacgag actcgaactc gcgaccccga
    17461 ccttggcaag gtcgtgctct accaactgag ctattcccgc ttgggtggtc tgtgccttac
    17521 agcactttca aattttggag cgggaaacga gactcgaact cgcgaccccg accttggcaa
    17581 ggtcgtgctc taccaactga gctattcccg cttaatcttc gtctttcgaa ccgctactgc
    17641 cttggctgcc ttcactcacc ttagtcacat acttttgtaa gttcccaagg attcgttcag
    17701 ttgccgcctt gtcgcaattc gaaatactgc gattgggtgg tctgtgcttt gcagcacttt
    17761 tcaaattttg gagcgggaaa cgagactcga actcgcgacc ccgaccttgg caaggtcgtg
    17821 ctctaccaac tgagctattc ccgcgtacta cttaattttg ctttcgtaat tttcgcattt
    17881 ctgcgtcgtt acgggaggcg cattatacga gaaatctttc cctctgcaac cccccaaaaa
    17941 agtttttttt gcgtttttat ctcaagtgct gaaattatca gcaagacgtt caatttagcg
    18001 tcaaaatcac gccgccgcaa gtggattccg gcgcttacgt gaccaggaaa aattttacag
    18061 cttgataaaa tgctcgcggt agtaagccag ctccgccacc gattcacgga tatcatccat
    18121 tgcctgatgc gtcccctgct tggtaaaacc atccagaatt tccggcttcc agcggcgcgc
    18181 cagctctttc agggtgctga catcgagata acggtagtgg aagtaggctt ccagctccgg
    18241 catgtattta aacaggaaac gacggtcctg tccgatgctg ttaccgcaaa tcggcgattt
    18301 tcccgcaggc acccactgtt ttaagaattc gagcgttgcc agttcagctt cccgatcgcc
    18361 catcgtgctc gctttcacgc gctctaccag cccgctggcg gtatgggtgc gcacgttcca
    18421 gtcatccatc agcgccagct gttcatcgga ctgatgtact gcaatggtcg gcccttctgc
    18481 cagaatattc agattggcat cggtcaccag cgtggcaatc tcaataatgc gatcgcgctc
    18541 gggatccaga ccggtcatct caagatcgat ccaaatcagg ttgttttcat tggcactcat
    18601 gctattttcc acccattcac agtgactata ttgatccaca attgcgtgta tcatagatgt
    18661 tttgcccatc aggggcgacc aggagtcagt acgattgagt aaaaataaac tctccaaagg
    18721 ccagcagcgc cgcgtgaacg ccaatcacca gcgtcgtctt aaaacgtcta aggagaagcc
    18781 cgactacgac gacaatctgt ttggcgagcc tgatgaaggt atcgtcatca gccgctttgg
    18841 tatgcacgct gatgtggaat ccgccgatgg cgacgttcac cgctgcaata ttcgccgtac
    18901 catccgttcg ctggtaaccg gcgaccgcgt agtctggcgt ccgggtaaac cggcggcgga
    18961 aggcgtaaat gtcaaaggga tcgtggaagc ggtgcatgag cgtacctcgg tgttgacgcg
    19021 tccggatttc tacgacggcg taaaacctat tgccgccaac atcgaccaga ttgtcattgt
    19081 ctccgccatt ttgccggagc tgtcgctcaa tattatcgac cgttacctag tggcctgcga
    19141 aaccttgcag attgagccga ttattgtgct caacaagata gacctgctgg acgacgaagg
    19201 catggcgttc gtcaacgagc agatggatat ctaccgcaat atcggttatc gcgtattgat
    19261 ggtttccagc catactcagg atgggctaaa accgctggaa gaggcgttga ccgggcgcat
    19321 cagcattttt gccgggcagt ctggcgtcgg caaatccagc ctgctgaacg cgttgctggg
    19381 gctgcaaaaa gagatcctga ccaacgatgt ctctgacaac tcggggcttg gtcagcacac
    19441 cacgaccgcc gctcggctgt atcacttccc gcacggcggt gatgtgattg attcccctgg
    19501 tgtgcgtgag ttcggcctct ggcacctgga gccggaacaa atcactcagg gctttgtcga
    19561 attccatgac tatttaggtc tgtgtaaata tcgcgattgc aaacacgata ccgatccggg
    19621 ctgcgctatc cgggaagcgg ttgaggaagg gaaaatcgcg gaaacccgtt tcgaaaacta
    19681 tcaccgtatt ctggaaagca tggcgcaggt aaaaacgcgt aaaaactttt ctgatacgga
    19741 tgactgacaa ttaagctaac cgtcgttaga atcgtcccct ttttttcagg atccggcatg
    19801 taggccgaat caggaacgac aaaacaatgg cctggaggct accttgttaa attcatttaa
    19861 actttcgcta cagtacattc tgccgaaact atggcttact cgcctggcgg gttggggcgc
    19921 aagcaagcgg gcaggatggt tgacaaaact ggttatcgat ctgttcgtta aatactacaa
    19981 ggtcgacatg aaagaggcgc aaaagccgga caccgccagc taccgcacct ttaacgaatt
    20041 ctttgtccgt ccgctgcgtg acgaagtacg cccaatcgat accgatccga atgtactggt
    20101 catgcctgcc gatggcgtta tcagccagtt gggtaaaatc gaagaagata aaatcctgca
    20161 agccaaaggc cacaactaca gcctcgaagc cctgctggca ggcaactatc tgatggcgga
    20221 cctgttccgc aacggtacgt ttgtgaccac ttacctctcc ccgcgtgact accaccgcgt
    20281 acacatgccg tgcaacggta ttctgcgtga gatgatctac gtgccgggcg atctcttctc
    20341 cgttaaccat ctcacggctc agaacgtgcc gaatctgttt gcccgtaacg aacgcgtgat
    20401 ttgccttttc gataccgaat ttggcccaat ggcgcagatt ctggtcggag cgacgattgt
    20461 tggcagcatt gagacggtct gggcgggcac cattacgccg ccgcgcgaag gtatcatcaa
    20521 gcgctggacc tggcctgccg gggaaaacga cggttctgtg gcactgctga aaggccagga
    20581 aatgggtcgc tttaaactcg gttccaccgt tatcaacctg tttgcaccgg gtaaagtgaa
    20641 tctggttgag caactggaaa gcctgtctgt cacgaaaatt tgccagccgc tggcagtatc
    20701 taccgaaacc tttgttacgc cagacgctga acctgccccg cttcctgctg aagagatcga
    20761 agcagaacac gacgccagcc cattggttga cgacaaaaaa gaccaggtct aatccatcaa
    20821 aggaaacgct gacgtgcgcc tgattatcac ttttctgatg gcctggtgcc tcagttgggg
    20881 ggcgtacgcc gcgacggccc ccgatagcaa acaaatcact caggaactgg agcaggcaaa
    20941 agcggcgaaa cccgcacagc cggaagtcgt agaggcgctt cagtctgcct taaatgcgct
    21001 tgaggaacga aaaggttccc ttgagcgcat caaacaatat cagcaagtta tcgataatta
    21061 tccgaaactc tccgctactc tgcgcgcaca attaaacaac atgcgtgaca agccgcgcag
    21121 cgtgtcgccg ggaatgtcta ccgacgcgct gaatcaggaa attctccagg tcagcagcca
    21181 gttgctggat aaaagccgtc aggcccagca agagcaggag cgcgcccgcg agattgccga
    21241 ttcgctgaat caactgccgc aacagcaaac cgacgcccgc cgtcagttaa atgagatcga
    21301 gcgccgcctg ggaacgctta ccggcaatac tccgctcaat caggcacaaa atttcgcgtt
    21361 gcagtctgac tctgcacgtc ttaaggcgct cgttgatgaa ctggagctgg cgcagctgtc
    21421 tgccaataac cgccaagaat tagcgcgctt acgctcagag cttgcggaaa aagagagcca
    21481 gcaactggat gcgtatttgc aggccttacg taatcagttg aatagccaac gtcagcttga
    21541 ggcggagcgg gcgctggaaa gtaccgaatt actggcagaa aacagtgccg atttgccgaa
    21601 agatatcgtc gcgcaattca aaattaaccg cgaactatcg gcagcgctga atcaacaggc
    21661 gcagcggatg gatctcgttg cctcgcaaca gcgtcaggct gccagccaga cgttacaggt
    21721 ccggcaggcg ttgaatacgc tgcgtgaaca gtcgcaatgg ctgggatcgt ccaatctgct
    21781 cggtgaagcg ttgcgagcgc aggtggcacg gctaccagaa atgccaaaac cacaacagct
    21841 tgataccgaa atggcgcagt tgcgtgtgca acggttacgt tatgaggatc tgcttaataa
    21901 acagccgctg ctacggcaaa tccatcaggc cgacggtcag ccgctgaccg ccgagcaaaa
    21961 ccgtattctg gaagcacaac tgcgcactca gcgtgagttg ctgaactcat tgttgcaggg
    22021 tggcgacacg ctactattgg aactgaccaa gctgaaagtc tccaacgggc aactggagga
    22081 tgcgctgaaa gaggtgaacg aagcaacgca ccgctatctg ttctggacct ctgacgtgcg
    22141 tccgatgacc atcgcctggc cgctggaaat cgcccaggat ctgcgtcgtc tcatttcgct
    22201 ggacaccttc agtcagttgg gcaaagccag tgtgatgatg ctgaccagca aagagacaat
    22261 tttgccgctg tttggcgcgt tgattctggt cggttgcagt atttactcgc gccgctattt
    22321 cacccgtttt cttgaacgtt cggcggcgaa agttggcaaa gtgactcagg atcacttctg
    22381 gctgacgttg cgcactcttt tctggtcgat tctcgtcgcg tcaccgttac cggtgctgtg
    22441 gatgacgctg ggttacggct tgcgcgaggc gtggccttat ccgctggcgg tcgcgattgg
    22501 cgatggtgta acggccaccg tgccgctgct gtgggtagtg atgatttgcg ccacctttgc
    22561 ccgcccgaac ggcttgttta tcgctcattt tggctggccg cgcgaacgcg tatctcgtgg
    22621 gatgcgctac tacctgatga gcatcgggct tattgtgccg ctgattatgg cgctgatgat
    22681 gttcgataac ctcgacgacc gtgaattctc cggttcgctg ggaaggcttt gctttatcct
    22741 catttgcggt gcgctggcgg tggtcaccct cagcctgaaa aaggccggga ttccgctgta
    22801 tctcaacaaa gaaggcagcg gcgacaacat taccaaccat atgctgtgga acatgatgat
    22861 tggcgcgccg ttggttgcca ttctggcgtc ggctgtgggt tatctggcaa cggcacaggc
    22921 gctgttagcg aggcttgaaa cctcggttgc catctggttc ctgctactgg tggtttatca
    22981 cgttatccgc cgctggatgc tgatccagcg ccgcaggctg gcgtttgacc gggcgaagca
    23041 tcgccgggca gagatgttag cgcaacgtgc gcgtggcgaa gaggaagcgc atcatcacag
    23101 tagcccggaa ggagcaattg aagtcgatga aagcgaagtc gatctcgatg ccatcagtgc
    23161 gcaatccttg cggctggtgc gctcaatttt gatgttgatc gccctgcttt ctgtcattgt
    23221 gctgtggtca gaaatccatt ccgctttcgg cttcctcgaa aatatttcgc tgtgggatgt
    23281 cacctccacg gtacagggcg tagaaagtct ggagccaatt accctcggtg cggtgctgat
    23341 tgccattctg gtgtttatca tcaccacgca gctggtgcgc aacctgcccg cgctgctgga
    23401 actggcgatt ttgcagcacc tggatttaac gccgggtacg ggctacgcca tcaccaccat
    23461 caccaaatat ctgctgatgc tgattggcgg gctggtcggc ttctcgatga ttggtattga
    23521 gtggtcgaaa ttgcagtggc tggtcgccgc gctcggtgtt ggtctcggtt ttggtttgca
    23581 ggaaattttc gccaacttta tctctggtct gattatcctg ttcgaaaaac cgattcgcat
    23641 tggcgatacg gtgacaattc gcgatctcac cggtagcgtg acgaaaatta acacccgcgc
    23701 caccaccatc agcgactggg accgtaaaga gataatcgtg ccgaacaagg cgtttattac
    23761 cgagcagttt atcaactggt cgctctctga ctcggtcacg cgcgtagtgt tgacgattcc
    23821 ggcccctgct gatgccaaca gtgaagaagt gacggaaatc ctgctcaccg cagcgcgtcg
    23881 ctgctcgctg gtgatcgaca acccggcacc ggaagtcttc ctggtggatc tgcaacaagg
    23941 gattcagatt ttcgagctgc gtatttatgc cgctgagatg ggtcaccgta tgccgctacg
    24001 ccatgagatc caccagctga ttctggctgg cttccatgcc cacggtatcg atatgccatt
    24061 cccgcccttc cagatgcgtc tggaaagcct caacggtaaa caaacgggga gaacgctgac
    24121 gtctgcgggc aaaggtcgtc aggcgggaag tttgtaatgc atgaagctgc caaatgatgt
    24181 caacgtggct catttagcag cttaacatca tcttcagtta tcaccggccc tggagagtat
    24241 catccatacg cattacttcc tgacaactca tcgccggaga gacgatgagt tgcaggattt
    24301 catatgatca gtcgagagct aactcaggtt tattcgccat gcgactgcaa tagttctggt
    24361 aaatcgccat cgctaacagc gagaagaaga cagggccgcc aatcatccac aatgtgctgt
    24421 cccagtctcc ggcttcaacc acaggctgaa taatggtgaa gacgttcgca aatgtcacca
    24481 ccagtacgac aaccaccatc gcaatcattg ccgacatacg cgttttgaaa atcacaaatg
    24541 gtctgtcgag atcctgacgt gctttaaaga atgggaaagc cagcgcgagg aacaggtaag
    24601 gaagcgtcat agacacgttc gccatcagcg tcagcttgtt aaagaacgcg gatgcggtac
    24661 cgccaccaaa cgaaaccagc aggatgaaga cagtaaccaa cccgcactgc atccacatgg
    24721 cgatagacgg catccccatc gcattcaggc gcgtcatcgg ttccggccac aacgctttcg
    24781 gcgtcccctg gatgatggct ttcagcggtg aatagcaaag cgtaaagaac gcaccggtat
    24841 aagcgaggaa catcgaaagt ccggtaatac gcgcaaacca tacgcccagc gacaatgacg
    24901 cttcaggtga caaatgcagt gcgttaccca gcgtcacccc gaggctcttc atcagcacat
    24961 aggtaatatt gccaaggtta acggaaccat tacttaatac ctgctgccag tttgtgctga
    25021 cgccccataa aaatattgcc agcgaataac cgattgaaat aacaatagcg gcaaaaacaa
    25081 tacctttggc aaagttcttt tctggatttt ccgttttatc gaccagacca ccaaccgctt
    25141 caattccgcc ataggcaaaa atagcaaata ccacaaacga tagcattgcc agaccggact
    25201 gataacccgg gttcggtgat gcaaggaaat taatatcctg cgcgaaatgc ccaccattta
    25261 ataacaaaat agtaatgctt actaacagca atactaaatt cagacacatt actgcaatac
    25321 cgcccaccgc agtaatgcgg gcaattttat taatcccctt tgaagcaacg acggtgacca
    25381 gaatcatcca tgctactgcc agcagaccaa ccacctgcgt aggctccagt ccggcaatac
    25441 gccagtgctg ggtcatgtcg ctaccataga ggaatgttga gaacggtacc caaactttcg
    25501 cggaggtact caccatccag atgatataag aggaaaacca cataaacgta ccaatgaagg
    25561 caaaacgtgg tccgacacta ttattcatcc aggaatagat accaccttct tctttgcgat
    25621 aagcagctcc catttcagcc atcatta
//
LOCUS       DAJVRF010000048        25646 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJVRF010000048 DAJVRF010000000
VERSION     DAJVRF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25646
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.048"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..699)
                     /gene="dgt"
                     /locus_tag="OQN13_001800"
     CDS             complement(<1..699)
                     /gene="dgt"
                     /locus_tag="OQN13_001800"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCS1615175.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKP"
     gene            783..1481
                     /gene="mtnN"
                     /locus_tag="OQN13_001801"
     CDS             783..1481
                     /gene="mtnN"
                     /locus_tag="OQN13_001801"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCS1615176.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            1474..2274
                     /gene="btuF"
                     /locus_tag="OQN13_001802"
     CDS             1474..2274
                     /gene="btuF"
                     /locus_tag="OQN13_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCS1615177.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            2312..2935
                     /locus_tag="OQN13_001803"
     CDS             2312..2935
                     /locus_tag="OQN13_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCS1615178.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(2982..3326)
                     /gene="erpA"
                     /locus_tag="OQN13_001804"
     CDS             complement(2982..3326)
                     /gene="erpA"
                     /locus_tag="OQN13_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCS1615179.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(3319..3384)
                     /locus_tag="OQN13_001805"
     CDS             complement(3319..3384)
                     /locus_tag="OQN13_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1615180.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(3408..4829)
                     /gene="clcA"
                     /locus_tag="OQN13_001806"
     CDS             complement(3408..4829)
                     /gene="clcA"
                     /locus_tag="OQN13_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCS1615181.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            5054..6334
                     /gene="hemL"
                     /locus_tag="OQN13_001807"
     CDS             5054..6334
                     /gene="hemL"
                     /locus_tag="OQN13_001807"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCS1615182.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(6492..8474)
                     /gene="fhuB"
                     /locus_tag="OQN13_001808"
     CDS             complement(6492..8474)
                     /gene="fhuB"
                     /locus_tag="OQN13_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCS1615183.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8471..9361)
                     /gene="fhuD"
                     /locus_tag="OQN13_001809"
     CDS             complement(8471..9361)
                     /gene="fhuD"
                     /locus_tag="OQN13_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCS1615184.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(9361..10158)
                     /gene="fhuC"
                     /locus_tag="OQN13_001810"
     CDS             complement(9361..10158)
                     /gene="fhuC"
                     /locus_tag="OQN13_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCS1615185.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(10209..12398)
                     /gene="fhuA"
                     /locus_tag="OQN13_001811"
     CDS             complement(10209..12398)
                     /gene="fhuA"
                     /locus_tag="OQN13_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCS1615186.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSTAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            complement(12618..15152)
                     /gene="mrcB"
                     /locus_tag="OQN13_001812"
     CDS             complement(12618..15152)
                     /gene="mrcB"
                     /locus_tag="OQN13_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCS1615187.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(15348..17777)
                     /gene="hrpB"
                     /locus_tag="OQN13_001813"
     CDS             complement(15348..17777)
                     /gene="hrpB"
                     /locus_tag="OQN13_001813"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCS1615188.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            17851..18381
                     /gene="thpR"
                     /locus_tag="OQN13_001814"
     CDS             17851..18381
                     /gene="thpR"
                     /locus_tag="OQN13_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCS1615189.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            18396..19100
                     /gene="sfsA"
                     /locus_tag="OQN13_001815"
     CDS             18396..19100
                     /gene="sfsA"
                     /locus_tag="OQN13_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCS1615190.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            19278..19733
                     /gene="dksA"
                     /locus_tag="OQN13_001816"
     CDS             19278..19733
                     /gene="dksA"
                     /locus_tag="OQN13_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCS1615191.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            19770..20696
                     /gene="gluQRS"
                     /locus_tag="OQN13_001817"
     CDS             19770..20696
                     /gene="gluQRS"
                     /locus_tag="OQN13_001817"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCS1615192.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            20735..22153
                     /gene="pcnB"
                     /locus_tag="OQN13_001818"
     CDS             20735..22153
                     /gene="pcnB"
                     /locus_tag="OQN13_001818"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCS1615193.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            22150..22629
                     /gene="folK"
                     /locus_tag="OQN13_001819"
     CDS             22150..22629
                     /gene="folK"
                     /locus_tag="OQN13_001819"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCS1615194.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            22999..23583
                     /locus_tag="OQN13_001820"
     CDS             22999..23583
                     /locus_tag="OQN13_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1615195.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            23688..24428
                     /locus_tag="OQN13_001821"
     CDS             23688..24428
                     /locus_tag="OQN13_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCS1615196.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            24463..25646
                     /gene="htrE"
                     /locus_tag="OQN13_001822"
                     /pseudo
     CDS             24463..25646
                     /gene="htrE"
                     /locus_tag="OQN13_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT         6287 a         6953 c         6392 g         6014 t
ORIGIN      
        1 cggctttttc attaaatagt gatgtgtctc aggcgtttcg ccacgccacc acgccggacg
       61 ggtatatttt aaaataccgc caacctgtgc ccaagtgaga ttcatccgca tcaatgtatg
      121 caccagacga atgccttgtg cattcccctc aaaatgacat aagtcctgac gaatcttgcg
      181 ccgcagctcg ttaagctgtt cttccccgtc ccgtaaacgt agtgccgcca cgctgcagcg
      241 atcgtcagtc agaggctggc tttcggcatc ttccgggtgc aaacgttggc gaaaccagtc
      301 atttatcgct gcttcgccaa aatgaccaaa cggcggattg ccgatatcgt gcatcaggca
      361 tgacatctca acaatgcttt caaaggggcc ggtcagttca tccaggccgt atgcttccag
      421 caatttaagc tctttcagac ggcttaaaat ttctttggcg atgtagcgtc ccacctgctg
      481 gacttccatc gagtgggtaa gacgcgtgcg cacggcggca ttgcgctcca gtggaaaaac
      541 ctgggtcttt tgttgcagac gacgaattgc cggagagttg atgatacgcc cgcgatcgct
      601 ctcgaagatc cgcaggatct catgttcggt tttaacgccc tgcggtgaac ggtaacgacg
      661 atgccagttt attttttttc ggaaatcaat ctgtgccatg tcctctcccg cgtgagaaat
      721 acgcttcccc gtaagcgcat ggtaaactat gctttcaaat cgggcttatc gcgagtaaat
      781 ctatgaaaat cggcatcatt ggtgcaatgg aagaagaagt tacgctgctg cgtgacaaaa
      841 tcgaaaaccg tcaaactatc agtctcggcg gttgcgaaat ctataccggc caactgaatg
      901 gaaccgaggt tgcgcttctg aaatcgggca tcggtaaagt cgctgcggcg ctgggtgcca
      961 ctctgctgtt ggaacactgc aagccagatg tgattattaa caccggttct gccggtggcc
     1021 tggcaccaac gctgaaagtg ggcgatatcg ttgtctcgga cgaagcgcgt tatcacgacg
     1081 cggatgtcac ggcatttggt tatgaatacg gtcagttacc aggctgtccg gcaggcttta
     1141 aagctgacga taaactgatc gctgccgctg aggcctgcat tgccgaactg aatcttaacg
     1201 ctgtacgtgg cctgattgtt agcggcgacg ctttcatcaa cggttctgtt ggtctggcga
     1261 aaatccgcca caacttccca caggccattg ctgtagagat ggaagcgacg gcaatcgccc
     1321 atgtctgcca caatttcaac gtcccgtttg ttgtcgtacg cgccatctcc gacgtggccg
     1381 atcaacagtc tcatcttagc ttcgatgagt tcctggctgt tgccgctaaa cagtccagcc
     1441 tgatggttga gtcactggtg cagaaactgg cacatggcta agtcactgtt cagggcgctg
     1501 gtcgccctgt cttttcttgc gccactgtgg ctcaacgccg cgccgcgcgt catcacgctt
     1561 tctcccgcca acactgaact tgcctttgcc gccgggatca cgccggttgg ggtcagcagc
     1621 tattccgact atcctccaca agcgcaaaag attgagcagg tttccacctg gcaggggatg
     1681 aatctggaac gcattgtcgc gctgaaaccc gatctggtga ttgcctggcg tggaggtaat
     1741 gccgagcggc aggttgacca gctggcttcg ctgggaataa aagtgatgtg ggtcgatgcg
     1801 acaagcattg aacaaattgc caatgcgtta cgtcaattgg ccccctggag tccgcaaccg
     1861 gataaggccg aacaagccgc gcaatccctg ctggatcagt acgcgcaatt gaaagcgcaa
     1921 tatgctgata aacctaaaaa acgtgttttt ctgcaattcg gcattaatcc gccatttacc
     1981 agtggaaaag agtcgattca aaaccaggta ctcgaagttt gtggcggaga aaacatcttt
     2041 aaagacagcc gggttccctg gccgcaagtt agccgcgaac aggtgttagc acgctcgcca
     2101 caggcgattg tcattacagg cggaccggac caaattccta aaatcaaaca atactggggt
     2161 gaacagctca aaattcccgt tattcctctc acgagtgact ggtttgaacg tgcaagccca
     2221 cgtattatcc tcgctgcaca acagctctgt aatgcgcttt cacaggtaga ttagcgcctg
     2281 acctttttag gtgctcagtg gggattgaac aatgctcgtc tattggctgg atatagtcgg
     2341 cacagcggta tttgccatct ccggcgtttt gttagccgga aaattgcgta tggacccttt
     2401 tggtgttctg gtactgggcg tggttaccgc tgtaggcggc gggacaattc gcgacatggc
     2461 gctggatcac ggcccggtat tttgggtgaa agatcccacc gatctggtcg ttgcaatggt
     2521 aaccagcatg ctgaccatcg tgctggtgcg ccagccaaga cgcttaccaa aatggatgtt
     2581 gccggtgctg gacgccgttg gtctggcggt gtttgtcggc attggcgtga ataaagcctt
     2641 taatgcggaa gtcggtccgt taatcgcggt ttgtatggtc gtcattactg gcgttggcgg
     2701 cgggatcatt cgtgatgtac tggcccgcga gatccccatg attttacgta cagaaatcta
     2761 cgcaactgcc tgtattatcg gcggtattgt ccacgctacg gcttattaca cattttccgt
     2821 accactggaa acagccagta tgatgggcat ggtcgtgacg ctattgattc ggctggcggc
     2881 tattcgttgg catcttaagc taccgacgtt tgcgctggat gagaatgggc gttgaagaga
     2941 gaagatgcgc cggatagttt atccggcaca tgaacaacag attagatgct aaaggaagaa
     3001 ccgcaaccgc aggtgctttt cgcgttcggg ttggtcacga tgaaacgaga accttccagt
     3061 ccttcggtat agtcaacgga accgccgacc agatattgca ggctcatcgg atcaaccacc
     3121 aagccaacgc cctgtttttc gatggtcata tcgccttcgt tcacctgatc atcaaaggtg
     3181 aaaccatact ggaagccgct gcaaccgcca ccggtgatat acacgcgtaa tttcagattc
     3241 gggttatctt cgtcagcgat caggctttta actttgttgg ctgctgcgtc ggtaaactcc
     3301 agcggcagtg ctacgtcatc actcatattt tgctccaaac gacatcggca attgggcaaa
     3361 ttctaaccca ataataatcg ccattatcta ataccctggt atttcgttca agtattctcg
     3421 ctggctgatg cggccttgct tcgcgccagt tgctcagctt cctgttttgc cagcgtgcgc
     3481 gcaagaatcg ccgagtatag cggtttcccg ccggtaaatt gcgctaatag tgttgcgcca
     3541 agaccggtaa taatcattgg caaaatgagc tggtagttat cggtcatctc cagaaccaga
     3601 atgatccccg ttaacggcgc gcgaatagat gccgccagta atgcccccat tccggcaata
     3661 gcaaacgtcc ccgcctcaag gtgatattgc ggaaacagct caacggcaac cattccgaaa
     3721 gcggttccca gcacagtacc cagcgccagc atcggggcaa aaataccgcc cggcgcgccg
     3781 gaagagaagc agagtaaggt ggtaatgacc cgagcgacga agataaacac cagcattccc
     3841 atgctgaaat tccccgcggt agcgatagga atcaggttaa aaccgccgcc cgacgttgct
     3901 ggtgccacaa accccagcaa tccacacaga ccgccaatcg caccgcccat cagtacccat
     3961 ttggtaatat tgccgccgtg cacacggtgc agcaaatcct gcatccccag cacccattta
     4021 ttaaaaatag ggccgaaaat gccaaaaata ataccgagga tcagataaag ccacagcgta
     4081 ttaagcggcg catcagaaag tttaccgacg tcaatcaacg caacttcatg attaaaaatc
     4141 cggtacataa tggtcgacat aatgacgcca ataaataccg cttttatcga aattaacgta
     4201 tagcgaaact gcggacgcat ctcttcgata ataaacaaaa tacccgccag cggcgcgtta
     4261 aaggccgcag ccagccccgc agcagcaccg gttgccagca gcgtatggcg agcttcgtca
     4321 cctttcaggc ggaaaatatc aagcaccata cggccaatgt taccgccgat ctgcacggtt
     4381 ggcccttcgc gccccaacac catgcctccg ccgagtgtcc ccagcccgcc aaagaacttc
     4441 accggcaata cacgccacca gcgaacggga cgttgatctt ccagcgcccc ttcaatttcc
     4501 gggatccccg aaccacctgc ttccggcgcg tatttgcgca ccaaaaagta gccaaacatc
     4561 gccagcaccg ccgaacagag aaaagcgacg gttaacagaa gcggataatt atcagcagta
     4621 tgtaccagcg cccccatacg ttggttctgc aaccaggcga cacctttgtc aaaagcaacc
     4681 gctgccagcc caacaagcgt gccgacgact gccgccataa acaaaatggc taacggggtt
     4741 ttatcgcgct caagaagttg gcgaatcagt tgtctgcgtc gcaggcgcgc ggcctgcggt
     4801 gtttctaaag agggagtatc agttttcatc caatgatcac ttattggtca tacaaataag
     4861 atgacggcat tttactcgca cgttccatga aaatcctttg caaagcgtaa tgtttcaaat
     4921 gcacaaaact tttatactct aagaaatcag gattgcacca gtacaagcag cctgatgttt
     4981 gacgagtatt taacttgtta tgaataacat agaatagcag ccattcactt tttctacgaa
     5041 tcaggaaccc tccatgagta agtctgaaaa tctttacagc gcagcgcgcg agctgatccc
     5101 tggcggtgtg aactcccctg ttcgcgcctt tactggcgtg ggcggcactc cactgtttat
     5161 cgaaaaagcg gacggcgctt atctgtacga tgttgatggc aaagcctata tcgattatgt
     5221 cggttcctgg gggccgatgg tgctgggcca taaccatccg gcaatccgca atgccgtgat
     5281 tgaagccgcc gagcgtggtt taagctttgg tgcaccaacc gaaatggaag tgaaaatggc
     5341 gcaactggtg accgaattgg tcccgaccat ggatatggtg cgcatggtga actccggcac
     5401 cgaggcgacg atgagcgcca tccgtctggc ccgtggtttt accggtcgcg acaaaatcat
     5461 taaatttgaa gggtgttacc acggtcacgc tgactgcctg ctggtgaaag ccggttctgg
     5521 cgcactcacg ttaggccagc caaactcgcc gggcgttccg gcagatttcg ccaaacatac
     5581 cttaacctgt acttataacg atctggcttc tgtacgcgcc gcgtttgagc aatacccgca
     5641 agagattgcc tgtattatca tcgagccggt ggcaggcaat atgaactgtg ttccaccgct
     5701 gccagacttc ctgccaggtc tgcgtgcgct gtgcgacgaa tttggcgcat tgctgatcat
     5761 cgatgaagta atgaccggct tccgcgtggc actggctggc gcacaggatt attacggtgt
     5821 ggaaccggat ctcacctgcc tgggcaaaat catcggcggt ggaatgccgg taggcgcatt
     5881 cggtggtcgt cgtgatgtaa tggatgcgct ggccccgacg ggtccggtct atcaggcggg
     5941 tacgctttcc ggtaacccaa ttgcgatggc agccggtttc gcctgtctga atgaagtcgc
     6001 gcaaccgggc gttcacgaaa cgttggatga gctgacaaca cgtctggcag aaggtctgct
     6061 ggaagcggca gaagaagccg gaattccgct ggtcgttaac cacgttggcg gcatgttcgg
     6121 tattttcttt accgacgccg agtccgtgac gtgctatcag gatgtgatgg cctgtgacgt
     6181 ggaacgcttt aagcgtttct tccatatgat gctggacgaa ggtgtttacc tggcaccgtc
     6241 agcgtttgaa gcgggcttta tgtccgtggc gcacagcatg gaagatatca ataacaccat
     6301 cgatgctgca cgtcgggtgt ttgcgaagtt gtgatccagc atggtttgtc ggatgcggcg
     6361 tgaacgcctt atccgaccta cgattcgggg ccgtttgtag gcccgataag acgcgccagc
     6421 gtcgcatcgg gcatccgttg tcgcgtaaac gctttatccg tccaggcgta cagggccgtt
     6481 atatggaaaa attaacggct ctgctttctc agcaaataga taaaatatgg cgcgccgata
     6541 aaggtcgaca gcagccccgc cgggatctgg aatggaaaca gcaccatccg cccacaccag
     6601 tcagcgaata ccagcagtaa accacccacc agcgccgaaa ttacgatgtg tggcatcgtc
     6661 cgtcgaaagc ccatcatccg cgcaatatgc ggtgccatta aaccaacaaa actcaacggt
     6721 ccaatagtca tcgtcgcggt cgccgtcagg caagccgcta acagcagcag cgcaattcgc
     6781 gtcggcgtca gcgccattcc tacggctcgt gcggtatcac cacccagcgg taaaatggtc
     6841 agccagcggc ggtacagcgg ggtaatcgcc agcaaaatca ccatcacaat tccggtacgc
     6901 cagacctgcg catcggtcgc gttgtaggtc gaaccggaaa tccaggtcag cacttgcgcc
     6961 attcgcgggt caccgctcgc ctgcaacatc atcaaaagca tggtgaatgc ggtacttaac
     7021 gccatccccg ccagtaacat acggtgtggg gaaaatccac cgcggccggc ggcgatcata
     7081 atgatcaaca gcgtcaccgc cgccccgata ctgcccgcgg gtaacagcca gccaaaggca
     7141 ttacccggca ccagaaacag cattaacacc acgccaaacg ccgcgccaga gctaatcccc
     7201 agcacttccg ggcttgccat cgggtttccg gtcagccgct gaataataca gcccgccacc
     7261 gccagcatga cgcccgcaaa cagcgccgcc ataattcgcg gccagcgcca gggcatcaaa
     7321 tcctcgagca acgccccgct cgcccacgtc cagccgtgcg catcacgacc aaacgacagc
     7381 gccaccacca cagccatcaa cagcagcacg ccgcccgcga gggcaaacgc cagcacatgt
     7441 tggcgttcag ccgcgacacg atcgttgacc ttcatatccg gcgcgctaat gctgcgtaaa
     7501 cgcggcaaca gccacagtag cagcggcgca ccgatcaacg cagtgaccga accggtggac
     7561 acttccatcc acacgcgagt cagccagagg atgatttgat cggaaagcca gaggatcagc
     7621 gccccaatca gcgacgccag catcaaccgt ggcaacaaac gccgcgcccc cagcattttc
     7681 gccagcagcg gcgcgaacaa accgataaag ccgataatcc ccaccgcatt caccagcagc
     7741 gcactgatga ctatcgccag cgacagcgcc gccagacgcg cgagcgacaa ggccagcccg
     7801 agattgcgcg ccacgccatc atcaagcccc atcagggtta acgggcgaag tagcaataac
     7861 gtcaacatca cgccgcccag caactgcggc cataaacgct caacgccgcc ccagtcggtt
     7921 tgcgtcagcg ttccggtgct ccacaggaac atgctttgca gctggtcgtg atggaagata
     7981 accagtagct gattaattgc cccgcaataa aggctcacca ccagccccgc gagaataagc
     8041 gtaaccggcg aaagccgttt cccccacgcg acgccaaaga cgattaagcc aacgacacaa
     8101 gcgcccacca gtgcggcaaa ctggctcgcc atagcacctg ggatcgccca aagtgtggtg
     8161 acggtaattc ccagttgcgc gcctgtagca acgccaagcg tcgtcggctc cgccagcggg
     8221 ttacgcagca cttgctgaaa cagcacgccc accagcccca ggcccgcgcc caccagcagc
     8281 gaaatcgcca gacgcggcaa caagctgtag tgaaaaatca tctgctcgat gacatcgatg
     8341 tctggcgacc aggcagcctg cgcccactgg ctacgcggca gcgcctgcga gaagttcatc
     8401 caggtgagcg ccgtagcgac aatcactaac agcgccagca ataacgccgg aaaaagcgca
     8461 attcgtttac tcacgcttta cctccgatgg cgttatccag aacgcgcaca aagtgcattg
     8521 ccgagagtgt cgcaccataa aaccagactg caggtacgcg ctgaaagcgt ccggcgcgga
     8581 caaacggcat ggcctgccac agcggggttg ccattagcgc atccatgtct ttgctgttgt
     8641 cgtgatcaaa acagagcaca tcaacgtctt tatacgctgc cagacgatcg atactgacgg
     8701 cggtactgcc ccagaagttg gtttcccctt gccaggcatt tgggatgccg tactcatcga
     8761 gaatttcctg gaacaagctg tttggaccga agaccagcat atggcgcgga tcgataagcg
     8821 tcgtcagcag taacgggcgt gcaccacgct tcacaaagcg gggtttcatg ctgcggataa
     8881 agtcttcgta ttgcgctaaa tgcgtttccg ctgcgctttg caggttaagt aaatctgcca
     8941 tttccgtcag cgatttacgc gccatcgcca acggctgttt gccgtcactg aagttaaatc
     9001 cgcgacccgg cgcaatacgt gccagcattt ctgatgaagg gccatatcct gccgaccaga
     9061 ccataaacga cggtttcatt tcggtcagca gttcaaggtt aggttctgta cgcaaaccga
     9121 cgtcaatcac tgagtccggc aatggtggtt cgctgaccca caggcgatag ttgatggtat
     9181 ccgccacgcc gtaaggcacg atgccgagcg ccagcagtaa ttccaccggc aaccactcca
     9241 gcgccacaat acgattgggg tcaatagccg ccgcgtgggc ggtattcatc tgccataaca
     9301 acggagaaag cgccatcgcc gttaacagtc gacggcgcga aataagaggt aagccgctca
     9361 tcaataaaca aaactcacag gtgcagcacc cgccggatgc ggcaaaatac ccatcgggat
     9421 gccataaatc atttcgaggg tttcgccgcg cataatttcc gcaggcgttc cctgagcaat
     9481 catttcacca ctgcgcaggg cgaccagata atcacagtag cgtgccgcca tattgatatc
     9541 gtgcaacacg gcaatgaccg tcaggccacg ttcctgactt aaacggtgca ccagcgccag
     9601 cacatcaacc tggtgggcga tatccagcgc cgaagtcggt tcgtcgagca acagacaacg
     9661 gctatcctgc gccaccagca tggcgatcca cgcccgctga cgttcgccgc cagagagact
     9721 atcgaccagc cgatgcgcca gcggttttaa gccaaccagc gagatagctt cctcgacctt
     9781 ttcgcgatct gccgcaccaa agcgccccag cgcgccatgc cacgggtaac gaccaatcgc
     9841 caccagttca cgcacggtca tcccttctgc cggaggaagc tgctgcggca aataagccac
     9901 tttgcgggca aacgctttgc tgctccagct ttccagcggc tgggcatcaa gaagaatctc
     9961 cccttctgac ggaggctgat gacgaccaag cattttgagc agagtggatt taccagaacc
    10021 gttgtgacca atcagaccgg tcactttccc ggcaggaaag gttaacgaca gcggatgcaa
    10081 aagcgtgcgc ccgggcacac gaaaggagat attacgcagt gcaaaagtgg tatcggaatg
    10141 attcgtgtat tcctgcataa cagccaactt gtgaaatggg cacggaaatc cgtgtcccaa
    10201 aagagaaatt agaaacggaa ggttgcggtt gcaacgacct gacgttctgc gccccagaag
    10261 cagccataag tctggaagca actggcaaca tactcacgat cgagcaaatt gttgacgttc
    10321 acagcaaggc tggatcccgc cataccaaag cgtgccagat catattttac aacagcatcc
    10381 attacagctg tgctacccac tttaaaagtg tttgccggat caccatagct ggaaccaata
    10441 aaacgaccac ctgttcccaa tgttaaacca gaaagcggcc cttcattgaa ggtataatcc
    10501 ccccagagag atgccatatg ctccggtact tgttctggag ttttaccttt gagattggtg
    10561 tctttggtgt attccgcatc ggtgtaggta taagaagccg tcaagttaat attggcattt
    10621 aacgccgctt ttgcttcaag ttccacacca cgagcgcgaa tctcacctgc tgggacttgc
    10681 gctaatgggt ttgttgggtc tgctgtcagg ttatttgttt tcgtcaattg atagactgcg
    10741 cccgtaacaa cgaccggcat atcattcgga acatatttta cgccagcttc atactgttca
    10801 cctttcgatg gcttatagga aatgcgcggg ttgctccaca gatcgaaagc actcggttca
    10861 aaggactggc tatagctaaa gtaaggtgaa ataccattat cgaataagta atttacacca
    10921 ccgcgccagg tgaactgatg atcattgcgc tcaatataac cttcagtcgg cgtataagag
    10981 ttttcacgaa cagtggtcgc ttgcttagac caatcgtagc gtccccccag agtgaatacc
    11041 catttattcc attccgcctg atcctgaaca taaatacctg tttgtttgct ttcattcatc
    11101 tggtatggct ctgcgctacc aaatgcaaag tattcagggt gatatttgtt ataaagatcg
    11161 atggatggtg cggatccaaa gctggcattg atatcattac gcatccgcat gaagtctacc
    11221 cctgtcagca aagtatgctc gacgtcacct gtagcaaact tactttcaag ttgggtatca
    11281 acgctaaagt tttgtagacg ctcattatcc accactgtcc cgcggtttag ggtatgacca
    11341 tcaccagcaa tccctgtgcc ataaacactt ttctgtgagg ttttcatttc actgaaacgc
    11401 agattctgac gcacggtgaa ggtgtcatta aaaccatgtt caaaactata tcccaccatt
    11461 ttctggttac gggagtatgt attattcgat gcaccttcat tgaagtcagt cggtagacgc
    11521 ttaccattag gcaatggctc aacggtcccc tctttcggca accaaccgta ataacccgtt
    11581 tctggttcat tctggaaata ggacaggaaa gtaaaattgg ttttgtcgtc tggacgccat
    11641 gagaatgatg gagcaatggt ataacgctga gattcagagt ttttctgctg ttcgtttgtt
    11701 gagcgagcga ggcccgtcaa acggtaagag aactcaccgt tatcatccag tgcatcactg
    11761 aaatcgaatc cggtctgaaa cagattatcc gtccccattt taaactgaat ttctttcagg
    11821 ggttcagtgg tcggacgctt actcaccata gaaataatcc caccaggatt actttttcca
    11881 tagagaacag acgtcggccc acgcatcagt tcaaccctct cgagcatgta aggatcaatc
    11941 acagcatcgt tataaaagtt gccctgaagt tttagtccat caaggtaatt attttgattc
    12001 agaccaacag atgagaaacc acgaatgata acaaaatcat atgtattaga agcaccacga
    12061 ctgctaaccg taacgccagg tgtataacct aatgcttctt ttacagactg aaattgacgc
    12121 atctgcatct cttcatttgt cacaaccgaa atagactgcg gcgttttttc aattggtgta
    12181 tccgttttag tcgcagtagc agagtgcttc gccgcaatag ttgcagcagg cccccatgcg
    12241 ctttcttgcg gcgcaggtgc agcggtaacg gtgatagtgt cttctttcgg ttcaaccgct
    12301 gcctgtgcat aaacagacat gccgctaacc gctgtggcta ctacaactgc gattttacgc
    12361 agtgagtgtt ttggctgagc agttttggaa cgcgccattg gtatatctct gatgtaaagt
    12421 gaatgataac gtaaacgaga atgattatta taaagataag ataatattcg aaacgctggc
    12481 gggttcgcaa gcggtacgca atctgagcgt tcctgaaggg ttaataaaca accagatgaa
    12541 aagaaagggt taatacttta ggtgggatgt tattttaccg gatggcaatt cgccatccgg
    12601 tatttcacgc ttagatgtta attactacca aacatatcct tgatccaacc ggctacaccg
    12661 tcgctgtctt tctgctcttg ctgagcaggt tgctgttgcg gctgttgctg cggctgagaa
    12721 gactgatcaa acggattgcc tgacggctgc tgctgcatct cgctctgctg gcacagcgat
    12781 tgcggatcgc tggtccagac cggcaagaca cgcatgccac cgctgcaaac aaagttgccg
    12841 tcgtagtcca cgcccatatc tgcaatatct tctggcggaa caagattcag cggcgttggc
    12901 gtctggttag ccagataacg ctgataaata gacattgccc cgctggcacc atacagtttg
    12961 gtcggctggt tgttatcacg gcctacccag gtgatggtca ccgtgctgcc gtcaatgccc
    13021 gcaaaccagg tatctacgtt attgttggta gtccctgttt tccctgccag atgcaggttc
    13081 gggtatttcg ccccaagttg acgacccgta ccacgttgta ccacctgctg catggtccat
    13141 agtgtcagat acgccgcctg cgccggaaca gcgcgttctg cctgcgggaa gctctgatac
    13201 agcaccttgc catcttccgc gattaccgaa cgcagtgcag aaagcggtgc acggttaccg
    13261 ccgctggcga tggtctggaa tgcctgcgcc acttcgattg gcgttaagtt caacgccccc
    13321 agcagcatcg ccggaaccgg atgcagctga tctttcggca cgcccagttt gatccaggtc
    13381 tccgtaactg caggcagccc cagcgccatc cccagattta ccgtcggcac gttcatcgaa
    13441 cgggtcaacg catccaccag catcactctg ccgctttcgc tataacgacg gtcatcgttc
    13501 tgcggtgacc agacctggcc attcggctga cgcagcgcga ttggcgcatc ggcaatccac
    13561 gtgttcaggc gatagatttt cggctggctt aaagccgtca gataagtcgc tggttttgca
    13621 agggaaccaa tcgaacgacg cgcctgcatc gcacggttgt agcccgcaaa ctgcggctca
    13681 gaacctccca ccatcgcacg aacttcacca ctaaagcggt cgacgaccac aattgcagtt
    13741 tcaagatcgc tcaacttacg ctgtttcttc agtgccggaa tgccttccac ggcggctttt
    13801 tctgccgcgt cctgggccac cgagtcaaag gtagtgaaga tcttcacgcc ggagagatct
    13861 tttaccttat cgcctaattt cgcctgcagc tcctgacgca ccagttgcat aaaggctggc
    13921 tgtggagaga ttaccccacc gcgcggctgt acgcccagag gacgcgcgct gagcatttca
    13981 tacagttcac catcgataat ctgctgttgt tgtagcaaac gcagcaccag attacgtcgc
    14041 tccagcgcca gtttcgggtt acgccacggg ttgtagatgg acgccccttt caccataccg
    14101 actaacagcg cctgctggtc gaggctgagc tcttctaccg ggcgaccaaa gtaatacaag
    14161 ctcgcaagcg ggaagccgcg gatttcgttg tcgccgctct gaccgagata cacctcgttc
    14221 atatacagct caagaatacg gtctttgctg taacgcgcgt ccatgatcag cgccatgtaa
    14281 gcttcgttcg ctttacgcca gtaagaacgc tcgctggaga ggaacaggtt tttcaccagc
    14341 tgttgcgtca gcgtactcgc tccctgcacc gtgcgtccgg cggtcaggtt tgccagcacc
    14401 gcacgtccga tggagtagag actgatccca tcatgctcgt agaaatgacg gtcttctgtc
    14461 gccagcaaag tatccaccag caagtccggg aaaccactgc gcggcacaaa cagacgctgc
    14521 tcaccgtttg gcgaagagat catggtgatc aggcgcggat caagacggaa gaaaccgaac
    14581 tgacggttgt tctccatatt gacgatcgtc gccagatgat cgccatcaaa ggtcagacgc
    14641 gcacgcacct gcccttcttt gctgtccggg aaatcaaacg gacggcggat catctcaatg
    14701 ctgttggcct gcacggtaaa ttcgccagga cgggtcattt tcgacacctg acgatactgt
    14761 gttgcctcca gcagcttcac catttcgttc ttgctgatgg tcatatctgg ttcaaggttg
    14821 accattcggc cataaactgc cgcgggcagt tgccagacct tgccatcaat acggctacga
    14881 attttttgat cgagataaac gccgtaaatg gcgatcagca cggcaaaaac gatagccagt
    14941 tttaacagca gccatagcca gccgcgtttg ccacgaggct tacgcccttt gcctttgccc
    15001 ttacctttgc gcggcatcgg ttcttcatcc tcatagtcat cataatcgtc gtaatcatcg
    15061 tcatcttcgt aacgacgacg gcttaccttt tgtttgaccg gacgcgtcgg tttccctttg
    15121 cgtccaattg gctcgcggtc attcccggcc atgctttttc tccgcaatat tcaggcgcaa
    15181 aagcccgatt ttctgttctt ctgttacaag acagaagaag atatctctca aaatttgtgc
    15241 tcgatcggtc ccctctccct tgagggagag ggttagggtg agggtgaacg tgcgcgctga
    15301 tgccctcacc ccggccctct cccacaggga gagggagaaa accaacctta cgaatacttt
    15361 ttcgtccgtc gcgtcggtgc cgtgtttgcc gggtcgtccg gccagacgtg tttgggataa
    15421 cgccctttca tctctttttg cacctcacgg tacgctcctt tccagaaggc gctcaaatct
    15481 cgtgtgattt gtagtggcct ttgggcgggt gaaagcaact ccagcaccag cggcacgccc
    15541 ccctgggcga tagtcggatt agtggcctca ccaaacatct cctgcattct caccgccagt
    15601 gcgggcgggt tatcttcatg ataacgaatg gcgatccggc ttcccgtcgg cacagtgtaa
    15661 tgcgcaggca attcactatc cagacgttgc tgcattcccc aatcaagtaa tccgcgtagt
    15721 gcctgataaa tgtccagtga tttcaggccg cgtagtgaat gtacgcctgt catatgtggc
    15781 agcagccacg tttccagcgc tgccaataaa ctttcatcat caaccgctgg ccagtcatat
    15841 tccggcaacc actttgcggc gcataacaaa cgcaagcgta gctgttccgc ttccgccgtc
    15901 cagttgagca cgcttaaacc tttatcacgg atgccattaa gcatcgcctg atgcaactcg
    15961 tcttctgacg gtttcgccag cggctgcact ttcaccgtca actgaccgat ttgtagccga
    16021 cgccaggctt tcagcgtacc ttgcgcgtca tcccattcca cggtgtcaga ttgctgcacc
    16081 agctgcggac agcgttgtac taattcatca atatcgaccg gtagcgccag taaaatccgc
    16141 gcatccggcg aggcgctgcc ctgcaataat aacggtgcga tcaaccattc gtggcggctt
    16201 agcgcgtcgt cggcatcgag catcgctccc atgccgtttg ccagttgata gcgtccatct
    16261 tgcccacggc gatgagcaat gcgatcggca aacgccccgg caagtagcgg cgcgataagc
    16321 gaactgtctg cctcaccgcc gcgtacgttt aagcgtttta acagttgctg actacgttgc
    16381 tgccaggctg gttgattgcg cgaaaacgcc acgcccaggt cactattgcc catcagtggc
    16441 ggctcttcga gaatggcggc aatttttgcc gcggtagcag cttcgtcgtc gttcttcgcg
    16501 ctaaccagca ttgccgctaa acgcggatcg ttacccagcg ctgccatttt ttgcccttgc
    16561 gcactaagcc gttcaccgtc cagcgccccc agcatttgta acagacgttt cgcggccatt
    16621 agattcacta ctggcggttg atccagccag ctcatctgcg ccggatcgct gcatccccat
    16681 tgcagtaatt ccatcagcaa accggaaaga tcgctttgta agatctccgg ttcactttgc
    16741 gcggcagcgc gttctgcttg ttctttggcg attaaatgca ggcagatacc cggctccaga
    16801 cgcccggcgc gcccggcacg ctgcgtcatg gatgcctggc taacgcgttg agtaatcagt
    16861 cgcgtaagcc ccgtgcgcgg ctcaaaacgc gccacacgct cctgggcaca atccaccacc
    16921 agacgaatac cttcaatggt taaactggtt tcagcaatat tggtcgccag caccactttg
    16981 cgcatccctt gcggtgccgg gaggatcgct tttcgctgat cgttcagcga caacgcgcca
    17041 tacagcgggc agagcaacac atcactgccg atgcgcgaag ccagttgttc ctgcacacgc
    17101 tgaatttctc cgacgccagg taaaaataac agtaatgatc cgctttcctg acgcagcatt
    17161 tcggcggtgg ctaccgcaac ggcttcgtca aaacgctgat gcgcgggcag cggtaaataa
    17221 tggcgttcaa ccggaaacga gcgcccttct gagatgacga cgggcgcttc tggcagcatt
    17281 tgctgcaagc ggtcgttatc cagcgtagcc gacataatca gcagtttaag gtcatcacgc
    17341 agaccttgtt gcacatcgag taacagcgcc aacgccaaat ccgcctgcaa gctgcgctca
    17401 tgaaattcat caaggatcac caatccaaca ccgctcaact ccgggtcacg ctggatcatc
    17461 cgcgtcagca cgccttcggt aactacttcc agacgggtat ttggcccgac gcagttttgc
    17521 gcacgcatcc ggtagccaac ggtatcgcct ggcttttcgt taagcagctc cgccagccgt
    17581 tgcgcgacgt tacgtgccgc cagacgacgc ggctccagca ggataatttt cccgttaatg
    17641 ccgggatgcg ccagcagttg cagcggcagc caggttgatt tcccggcccc ggtcggcgca
    17701 cttaataata cctgcggcgc acaatcgaga gcggtaagta attcaggtaa gacggcagca
    17761 acgggcaacg acgacacaaa acgctccaga gggttaacat tcttcgcgcc acattgtagc
    17821 atcgcggtaa ttcataaccg agtgcctcac atgtctgaac cgcaacgtct gttctttgct
    17881 atcgacttac ctgcagaaat ccgcgaacag attatccact ggcgcgccac acacttccca
    17941 cctgaggcgg gacgtccggt cgccgccgat aatttgcatc tgactctggc atttttaggc
    18001 gaagtgagcg ccgagaaaga gaaggcgctt tctcttttag ccggacggat tcgtcaacct
    18061 ggtttcacac tcacgcttga tgacgccgga caatggctgc gttcgcgtgt ggtgtggtta
    18121 gggatgcgtc agccgccacg cggcttaatc cagctggcga atatgctccg ttcacaggct
    18181 gcccgcagcg gttgttttca aagcaatcgt ccgtttcatc cacatattac cttattgcgc
    18241 gacgccagcg aggcggtgac aatcccgccg ccaggtttta actggtctta tgcggtgacg
    18301 gagttcaccc tttacgcctc ctcgtttgcc cgtggacgca cacgctacac gccgctaaaa
    18361 cgctgggcgc taacgcaata acaaggattg tcgcaatgga attctctccc cctctacagc
    18421 gcgcgacgct aattcagcgt tacaaacgtt ttttagccga tgtgatcaca cccgatggtc
    18481 gcgaattaac gctacactgc ccgaatacgg gtgcgatgac cggttgtgca acgcgtggcg
    18541 ataccgtctg gtattcgact tcagacaaca ccaaacggaa atacccacac acctgggaac
    18601 taactcaaag ccagagcggc gcatttattt gcgtcaatac gctttgggct aacaggttga
    18661 cgaaagaggc tatccttaat gaatcaattt cagaactgtc aggctatagc tcgctgaaaa
    18721 gcgaagtaaa atacggcgca gaacgcagcc gtattgactt tatgttgcag gcggattcgc
    18781 gtccagactg ctatattgaa gtgaaatcgg ttacgttagc ggagaacgaa cagggatatt
    18841 ttcccgatgc ggtcactgaa cgaggtcaga aacaccttcg ggagttgatg aacgtagcgg
    18901 ctgaaggcca gcgtgcggtt atctttttcg ccgtgctgca ttcagccatt acacggtttt
    18961 cacccgcgcg ccacatcgat gagaaatacg cgcaactatt gtcagaagct caacagaggg
    19021 gggtagaaat tctggcttac aaagcggaaa tttctgctga aggcatggct cttaaaaaat
    19081 cactgtcggt tacattgtag taaagtaagt aactggttaa tttacattct ggtcgcgtgc
    19141 gcaaatacgc ttttcctcac acagttgtca agtgttacgt ttagataatt gctatccgga
    19201 aaagcatctg ctatttatag cggcctcatt tttcccccga acatggggat cgatagtgcg
    19261 tgttaaggag aagcaacatg caagaagggc aaaaccgtaa aacatcgtcc ctgagtattc
    19321 tcgccatcgc tggggtggaa ccatatcagg agaagccggg cgaagagtat atgaatgaag
    19381 cccagctggc gcacttccgt cgtattctgg aagcatggcg taatcaactc agggatgaag
    19441 tcgatcgcac cgttacacat atgcaggatg aagcagccaa cttcccggac ccggtagacc
    19501 gtgcagccca ggaagaagag ttcagcctcg aactgcgtaa ccgcgatcgc gagcgtaagc
    19561 tgatcaaaaa gatcgagaag acgctgaaaa aagtggaaga cgaagatttc ggctactgcg
    19621 aatcctgcgg tgttgaaatt ggtattcgcc gtctggaagc gcgcccgaca gccgatctgt
    19681 gcatcgactg caaaacgctg gctgaaattc gcgaaaaaca gatggctggc taattacagc
    19741 cgttccatca cgtttaccac aggcgggaaa tgctcccgcc ttattttttg ttcaaagaga
    19801 tgacagacac acagtatatt ggccgcttcg ccccctctcc ttccggcgag cttcattttg
    19861 gctctctgat cgccgcgctc ggcagctatt tgcaggctcg cgcccggcaa ggtcgctggc
    19921 tggtacgcat agaagatatc gacccgcctc gtgaagttcc cggtgccgca gaaactatcc
    19981 tgcgccagct ggaacattac ggtctgcact gggacggcga tgttctctgg caatcgcaac
    20041 gtcacgacgc ctatcgtgaa gcactcgcct ggttacatga acaaggacta agttattact
    20101 gcacctgtac gcgtgcgcgt attcaaagca ttggcggtat ttacgacggt cattgccggg
    20161 tgttgcatca tggaccagac aatgccgcag tgcgtatccg ccagcagcat ccggtcacgc
    20221 aatttactga ccagctgcgc ggcattattc acgccgacga aaaactggca cgggaagatt
    20281 ttatcattca tcgccgtgat gggttgttcg cctacaacct ggctgttgtg gttgatgatc
    20341 atttccaggg cgttacagaa atagtgcgtg gggctgatct gattgaacca acagtaaggc
    20401 aaatctcgct gtaccagctt tttggctgga aagtgccaga ttacattcat ctgccgctgg
    20461 cgcttaatcc acaaggcgct aaactttcca agcagaatca tgcgcctgcg ttgccgaaag
    20521 gcgatccacg tccggtacta atcgcggcac ttcaatttct gggtcagcag gcagaagcac
    20581 actggcagga tttcagcgtc gaacaaatcc ttcagtcagc agtcaaaaac tggacgctga
    20641 ccgccgtgcc cgagtcggca attgtaaatt caacattctc aaatgcgtca tgctgagcta
    20701 tgattagccg ctattttttt gtcctgaatg atgtttgaca ctaccgaggt gtactatttt
    20761 tacccgagtc gctaattttt gccgcaaggt gctaagccgc gaggaaagcg aggctgaaca
    20821 ggcagtcgcc cgtccacagg tgacggtgat cccgcgtgag cagcatgcta tttcccgcaa
    20881 agatatcagt gaaaatgccc tgaaggtaat gtacaggctc aacaaagcgg gatacgaagc
    20941 ctggctggtt ggcggcggcg tgcgcgacct gttacttggc aaaaagccga aagattttga
    21001 cgtaaccact aacgccacgc ctgagcaggt gcgcaaactg ttccgtaact gccgcctggt
    21061 gggtcgccgt ttccgtctgg ctcatgtaat gtttggcccg gagattatcg aagttgcgac
    21121 cttccgtgga caccacgaag gtaacgtcag cgaccgcacg acctcccaac gcgggcaaaa
    21181 cggcatgttg ctgcgcgaca acattttcgg ctccatcgaa gaagacgccc agcgccgcga
    21241 tttcactatc aacagcctct attacagcgt agcggatttt accgtccgtg attacgttgg
    21301 cggtatgaag gatctgaagg acggcgttat ccgtctgatt ggtaacccgg aaacgcgcta
    21361 ccgtgaagat ccggtacgga tgctgcgcgc ggtacgtttt gccgccaaat tgggtatgcg
    21421 catcagcccg gaaaccgcag aaccgatccc tcgcctcgct accctgctga acgatatccc
    21481 accggcacgc ctgtttgaag aatcgcttaa actgctacaa gcgggctacg gttacgaaac
    21541 ctataagctg ttgtgtgaat atcatctgtt ccagccgctg ttcccgacca ttacccgcta
    21601 cttcacggaa aatggcgaca gcccgatgga gcggatcatt gaacaggtgc tgaagaatac
    21661 cgatacgcgt atccataacg atatgcgcgt gaacccggcg ttcttgtttg ccgccatgtt
    21721 ctggtaccca ctgctggaga cggcacagaa gatcgcccag gaaagcggcc tgacctatca
    21781 cgacgctttc gcgctggcga tgaacgacgt gctggacgaa gcctgccgtt cactggcaat
    21841 cccgaaacgc ctgacgacgt taacccgcga tatctggcag ttgcagttgc gtatgtcccg
    21901 acgtcagggt aaacgcgcat ggaaactgct ggagcatcct aagttccgtg cggcttatga
    21961 cctgttggcc ttgcgagctg aagttgaacg taacgctgaa ctgcagcgtc tggtgaaatg
    22021 gtggggtgag ttccaggttt ccgcgccacc agatcaaaaa gggatgctta acgagttgga
    22081 tgaagagccg tcaccgcgtc gccgtactcg tcgtccacgc aaacgcgcac cgcgtcgtga
    22141 gggtaccgca tgacagtggc gtatattgcc ataggcagca atctggcctc tccgctggag
    22201 caggtcaatg ctgccctgaa agcattaggc gatatccctg aaagccacat tcttgccgtt
    22261 tcttcgtttt accgcactcc accgctgggg ccgcaagatc aacccgatta cctcaacgcc
    22321 gccgtggcgc ttgaaacttc gctcgccccg gaagagttac tcaaccatac acagcgcata
    22381 gaactgcaac agggtcgtgt tcgtaaagct gaacgctggg gaccacgcac gctggatctc
    22441 gatatcatgc tgtttggtaa tgaagtgata aataccgaac gcctgaccgt tccgcactac
    22501 gatatgaaga atcgtggatt tatgctgtgg ccgctgtttg agattgcacc ggagctggcg
    22561 tttcctgatg gggagacgtt gcgtgaagtc ttacatacaa gagcatttga cgaattaagc
    22621 aaatggtaat ttatttatat tacgaatata aatattttct gaaaatcata catcctctct
    22681 ttccttaata aaaattttag tttaaacatt tttgcagaaa cataatataa attcccgcag
    22741 ataaatatca atcccatcgc cataccctaa ccaattgcac ttgtgaattt atttttgctt
    22801 aattttgaat taagaaaatt taatttgttc aaaagccctt aaagtgcaca ataggcgtcc
    22861 taatgaacaa tggttgtaaa ttttggtaac aaatatcatt gctcattaat atctagcatc
    22921 tgcttataac atttgtgtca tgaatagtca ttcttcaaac cagtagtctg actaatggaa
    22981 ttttattcaa ggatttttat gtctaaaaaa ttaggttttg ccctgtctgg tcttatgctg
    23041 gcaatggttg caggtactgc ttctgctgat atggatggtg gtcagttaaa tatcagtggt
    23101 ctggttgttg ataacacctg tgaaacccgc gttgacggcg gtaataaaga tggtctgatc
    23161 ctgctgcaaa ctgcaactgt tggtgaaatc gatgctggcg tgctgaatga caccgttggc
    23221 gctaaagcca aacctttcag catcaccgtt gactgcagca aagcaaatcc gaatccaggt
    23281 agtacagcta aaatgacctt cggttctgtt ttctttggta atagcaaagg aacgctgaac
    23341 aacgacatgt ctattaacaa cccgtctgac ggcgtgaata tcgctctgca caatattgat
    23401 ggttccacta tcaaacaggt tcaaattaac aaccctggcg atgtgtatac taaagccctg
    23461 gatgcaacga caaaatctgc tgtttatgat tttaaagcgt cttacgttcg tgccgtcgca
    23521 gaccaaacag caactgctgg ttatgtaaaa actaacactg catacaccat tacttatcag
    23581 taagtattca ccaattaaat tgattgaatg aatatacagg gaatagtgtt aataatttct
    23641 atttttatat tattccctgt tttaattaac tctgtcaggg atggtttatg ttttttaaca
    23701 ccaaacatac aacagcttta tgcttcgtaa cctgtatggc ttttagttca tcgtctattg
    23761 cggacattgt catttcgggt actcgcgtaa tatataaaag cgatcaaaaa agtgtcaacg
    23821 tacgtctgga aaataaaggg aataacccgt tgcttgtcca gagttggtta gatactggcg
    23881 ataacaacgc tgagcctggc agtattacag tcccttttac tgccacgccg ccagtatcgc
    23941 gtattgatgc caaacgtggg caaacaatca aattaatgta cacagccagc acctcactgc
    24001 ctaaagacag agagagcgtg ttctggttta acgtactgga agttccacca aaaccagatg
    24061 cagaaaaggt cgcgaatcaa agtctgctgc aactggcatt tcgcacacgt ataaaacttt
    24121 tctatcgccc ggatggattg aagggaaatc cctctgaagc cccgttagcc ctgaagtggt
    24181 tctggtcagg ttcagaaggt aaggcgtcat tgcgagtgac caatccaacc ccttactacg
    24241 tctcttttag cagtggtgat ttagaagcta gcggtaaacg ctatccgatt gatgtgaaaa
    24301 tgattgcacc atttagtgat gaggtcatga aagtcactgg ccttaatggc aaagcaagtt
    24361 ctgcaaaagt acatttttac gccattaatg attttggtgg cgccattgaa ggtaatgcca
    24421 gtctgtaatc aggcaaggat ataattccgc aggaagcata acgtgactat aaaaccaact
    24481 aaaaattatc ataatcatct gacccgtatc gctacgtttt gcgcgctgct gtattgcaat
    24541 agtgctttct gtgctgaact tgttgaatat gaccatacct tcctgatggg gcagaatgcc
    24601 tcaaatattg atctcagccg gtatagtgaa ggtaaccctg ctataccggg tatgtacgac
    24661 gtcagtgttt atgtcaacga tcaacctatt attaaccaaa gcattacatt tattgaaatt
    24721 gaaggaaaaa aaacgcacaa gcctgtatca cacttaagaa tttattgcag tttcatataa
    24781 attctcccga tataaataac gaaaaagccg ttctgcttgc cagggatgaa acactcggca
    24841 attgccttaa tttgacggaa attatccctc aggcttctgt tcgttatgac gttaacgagc
    24901 aacgtctgga tatagacgtt cctcaggcat gggtaatgaa aaattaccaa aactatgttg
    24961 atccatcgtt atgggaaaat ggcattaatg cggccatgtt gtcatacaac ctcaacggat
    25021 atcatagtga aactcctggt cgaagaaatg acagcattta tgctgctttt aacggtggga
    25081 tgaatttagg tgcatggcga ctgcgtgcct caggcaacta caactggatg accgattctg
    25141 gcagtaatta tgattttaag aatcggtata tacagcgtga tatcgcctcg ctgcgttctc
    25201 aactcattct tggtgagtct tatacaacgg gcgaaacctt tgattctgtc agtatccgcg
    25261 gcattcgttt atacagcgac agccgcatgt tgcctccgac attagcgagt tttgcgccta
    25321 tcattcatgg cgttgctaat accaacgcca aagtcactat tacgcaaggt ggctataaga
    25381 tttatgaaac gactgtgccg ccaggcgctt tcgtcattga tgatctgagt ccgtcaggtt
    25441 acggcagcga tcttattgtt actgtcgaag aatccgatgg ctcaaagcgg acattctcgc
    25501 aacctttttc atcggtcgtt caaatgttac gccctggcgt tggacgttgg gatattagcg
    25561 gcggccaggt cttaaaagat gatattcagg atgagcctaa tttatttcag gccagctact
    25621 actacggtct gaataactat ctaacg
//
LOCUS       DAJVRF010000049        25577 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJVRF010000049 DAJVRF010000000
VERSION     DAJVRF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25577
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.049"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..786)
                     /locus_tag="OQN13_001823"
     CDS             complement(<1..786)
                     /locus_tag="OQN13_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HCS1615197.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLK"
     gene            complement(867..3500)
                     /gene="yebT"
                     /locus_tag="OQN13_001824"
     CDS             complement(867..3500)
                     /gene="yebT"
                     /locus_tag="OQN13_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCS1615198.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(3469..4752)
                     /gene="yebS"
                     /locus_tag="OQN13_001825"
     CDS             complement(3469..4752)
                     /gene="yebS"
                     /locus_tag="OQN13_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCS1615199.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            4882..5379
                     /gene="msrC"
                     /locus_tag="OQN13_001826"
     CDS             4882..5379
                     /gene="msrC"
                     /locus_tag="OQN13_001826"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCS1615200.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            5476..6174
                     /gene="proQ"
                     /locus_tag="OQN13_001827"
     CDS             5476..6174
                     /gene="proQ"
                     /locus_tag="OQN13_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCS1615201.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDALRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            6194..8242
                     /gene="prc"
                     /locus_tag="OQN13_001828"
     CDS             6194..8242
                     /gene="prc"
                     /locus_tag="OQN13_001828"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCS1615202.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            8434..9315
                     /gene="htpX"
                     /locus_tag="OQN13_001829"
     CDS             8434..9315
                     /gene="htpX"
                     /locus_tag="OQN13_001829"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCS1615203.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(9361..10424)
                     /locus_tag="OQN13_001830"
                     /pseudo
     CDS             complement(9361..10424)
                     /locus_tag="OQN13_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            10601..11392
                     /gene="kdgR"
                     /locus_tag="OQN13_001831"
     CDS             10601..11392
                     /gene="kdgR"
                     /locus_tag="OQN13_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCS1615204.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(11535..11774)
                     /locus_tag="OQN13_001832"
     CDS             complement(11535..11774)
                     /locus_tag="OQN13_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1615205.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            11933..12076
                     /gene="mgrB"
                     /locus_tag="OQN13_001833"
     CDS             11933..12076
                     /gene="mgrB"
                     /locus_tag="OQN13_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCS1615206.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            12151..12438
                     /locus_tag="OQN13_001834"
     CDS             12151..12438
                     /locus_tag="OQN13_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCS1615207.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            13108..13251
                     /locus_tag="OQN13_001835"
     CDS             13108..13251
                     /locus_tag="OQN13_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCS1615208.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            13264..13473
                     /gene="cspE"
                     /locus_tag="OQN13_001836"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             13264..13473
                     /gene="cspE"
                     /locus_tag="OQN13_001836"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS1615209.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            13639..14448
                     /gene="rlmA"
                     /locus_tag="OQN13_001837"
     CDS             13639..14448
                     /gene="rlmA"
                     /locus_tag="OQN13_001837"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCS1615210.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(14445..15011)
                     /gene="mntP"
                     /locus_tag="OQN13_001838"
     CDS             complement(14445..15011)
                     /gene="mntP"
                     /locus_tag="OQN13_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCS1615211.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(15440..15898)
                     /locus_tag="OQN13_001839"
     CDS             complement(15440..15898)
                     /locus_tag="OQN13_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCS1615212.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(15953..16804)
                     /gene="manZ"
                     /locus_tag="OQN13_001840"
     CDS             complement(15953..16804)
                     /gene="manZ"
                     /locus_tag="OQN13_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCS1615213.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(16817..17617)
                     /gene="manY"
                     /locus_tag="OQN13_001841"
     CDS             complement(16817..17617)
                     /gene="manY"
                     /locus_tag="OQN13_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCS1615214.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(17680..18651)
                     /gene="manX"
                     /locus_tag="OQN13_001842"
     CDS             complement(17680..18651)
                     /gene="manX"
                     /locus_tag="OQN13_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCS1615215.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            19114..20664
                     /gene="yoaE"
                     /locus_tag="OQN13_001843"
     CDS             19114..20664
                     /gene="yoaE"
                     /locus_tag="OQN13_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCS1615216.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(20668..22266)
                     /locus_tag="OQN13_001844"
     CDS             complement(20668..22266)
                     /locus_tag="OQN13_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1615217.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(22397..23761)
                     /gene="sdaA"
                     /locus_tag="OQN13_001845"
     CDS             complement(22397..23761)
                     /gene="sdaA"
                     /locus_tag="OQN13_001845"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS1615218.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(23945..24523)
                     /locus_tag="OQN13_001846"
     CDS             complement(23945..24523)
                     /locus_tag="OQN13_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCS1615219.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(24527..>25577)
                     /gene="pabB"
                     /locus_tag="OQN13_001847"
     CDS             complement(24527..>25577)
                     /gene="pabB"
                     /locus_tag="OQN13_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCS1615220.1"
                     /translation="GALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQ
                     RHTVSLLSHNDVNARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHS
                     GDCYQVNLAQRFHATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCD
                     NSEIQTRPIKGTLPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGS
                     VKVPELFVVEPFPAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIID
                     ELEPQRRNAWCGSIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQ
                     ETFDKVNKILRQLEK"
BASE COUNT         6598 a         6298 c         6484 g         6197 t
ORIGIN      
        1 tttcagccac aggcaaacgg cttcgttttc ttcctggttt aaggtacagg tcgagtaaac
       61 cagcgtaccg ccagggcgta atgcatgaaa ggcgctgtcg atcagttccc gttgggtagc
      121 tgcgatttcc tgattgcttt ctggtgacca gttttttagc gcatcgggat ctttacgcac
      181 cacgccttcg ccggagcagg gagcgtccag caaaatggca tcaaacattt ctggcactgc
      241 cgcaccaaac acgcggccat caaaatgtgt gagcgcaaca ttactgatac cacagcggct
      301 gatattggca tgtaacactt ttacccgact ggcggaaaac tcattggcaa gaattgcccc
      361 ttcgttattc atccgcgcgg aaatttgcgt cgttttggag ccaggcgcgg cagcgacatc
      421 catcacccgc tgtggtgcat taccgtcagc aaacaaggcg gcgacgggca acattgaact
      481 ggcttcctga atataaaaca ggccacttaa atgctcggcg gtactaccca atggcaatgc
      541 atcttcattg tcgcgttcaa tccagaaacc ttcttcacac cacggaattg gcgtaagcgt
      601 ccagccataa ggagcggtta attgcaggaa atcagcaacg gagattttca gcgtattaac
      661 gcgaatgctg cggcgcaacg ggcgctgaca ggcggcaaga aaatcatcaa aagagagcgt
      721 cgaaggcatc gcttcgcgca tttgcgtcag aaaggcgtcc gggaaataaa cggtgtgttg
      781 ggccacgagc atataccact ggcaaaaaaa acaggcgcgc agtttagcat aaacgctccg
      841 gcgcaggcac gccggagcag tgggcattat ttgggaagcg cagtacccca ttcacgccac
      901 tctttcggtt cactttcctg caacaggaag tgcttgcctt cctgggcttt cggtgccagt
      961 ggcgtacccg gaggcgtggc gaaggcgatg ccgccacgga taaactggtt aaaggtgccg
     1021 gttttcacta cgccgcccgt cagaccaaag tccagactgt aacccgatgc caaccagaag
     1081 acggaattgt tacgcaccag gtgttgatag cgtttactga tgcgcatcgc aatcatcacg
     1141 cgatctgaca atgtccccag cgtcattccg gtaaccgtac ctacttccag accacggaac
     1201 agcacaggcg taccaatacc taacgaaccg gcttccggtg cttcgacaat aatgcttaag
     1261 ccatccaggt aacgcgaatc agtaatagta gcttcttgca actcaaagtc acggcgagga
     1321 ttaccccgac ctggttcgac attgatatac ggctggagga tagtatctag atgctcaacg
     1381 cccgcggctg aaatttgcgg tgtgaccacc gagaagcgcg taccgccgcg ggcgaaggtc
     1441 tggacatact ccggatagag caccgccttt gcctgtactt cattgcgcgc ggtaatcaga
     1501 tccagcgtct ggatttgccc gatatcaata ccgagatagc gaattggcat cccgaccgcc
     1561 agttttccgg catcgaaagc gtgaagcgta atctgcccac caaccgcacg ggccgctgtt
     1621 tcggaagcat acagaattcg tttgtcgcct ttacgctgac tggcgctggc accgctgagg
     1681 ttatcgaagc taatggctcc ctttaatgct ctggagagcg gggatgcctg tacggtcagg
     1741 ccactaccat tcagctgaac tttcgccccg ccttctgccc agaacacgct attgctggtc
     1801 agaaggttgc gatactccgg cttaatatgc agatcgatat caaacgcgtt agctcgcgga
     1861 cgcacggtaa tcacttcacc aacttcaaat ttacggtaca gcactaccga tcctgcctgc
     1921 acatccggca gcgtctctgc actcaaactc acggttgtgg tgggtaaatc gctaaggctg
     1981 ttctccagcg ctttttccag attggcatac agtggatagc tggctttcat ctcaccttta
     2041 tcgcctggca gaatacgtat cccgccgtta atccattctg aggcgctggc accaagaaac
     2101 tcaacgccat ccagccccac cttcacgtcg acacggctgt tgacgacaaa tttgctatcg
     2161 ccttttacca gttcgcgatg ctgaggctcg atggcgacgg taaaggtaac gcctttgctg
     2221 gtgagtttac gatcaataac ctggcctacc tgcacgccgt gaagaatgag cggctgacca
     2281 gcatcaatac cgtaactttc cggcgcggtc agggtcagcg tcagaacatc aggttcatgc
     2341 agcagtgctt tttcgcctgg cacaacaacg aactctttgc gtggctcgcc atcgccgggt
     2401 accaactcga aggttttgcc agtcagcagg gcgctgagat tggcatcgct aagggataat
     2461 ttcggattaa gtaattcgat gcgggtattt tcacgaagca gggtaacgac gctgggatta
     2521 actgtcattt ccccagtgac tttaccacca ggatttaaat ccagtttagt tagctgtccg
     2581 acttccagtc cctgatacat taatggcgtc gagtcggcgg ttaatccggc cccactcggc
     2641 agttccagtt tgattattac gccacgctgg ctatgggcga ggtcttcata cagaccaaag
     2701 gtatcttccg cctcggcagg tttcgactct tcaggtgaat cgaaggcaat cgcaccgtta
     2761 accagtgccg ccagactttc cagtttcacc ttcgcaccac tgatactgac gttggcatca
     2821 acgccggaaa cgttccagaa acggctacct tttttcacta ggtcggtaaa acgccgctcg
     2881 atcaggacat caatcaccac gccttgcttg ttgggattga tggcatagtc gtagactttt
     2941 cccacaggga tcttgcggaa atagaccaat gaaccgctgt tcagcgaacc gagatcgggg
     3001 gcttgcaggt ggatcatcag atcgccattg tccagtcgat atttcggttg ggtatcgagt
     3061 gcgacaaagt gatcctgctc tttaccttta cctggcatca tgccgatata gttaccaccg
     3121 acgagggcgt ccagcccgga gacacctgcc aacgatgctt ttggcgtcac cagccagaac
     3181 tgagtctctt cgcgcagcgc atctttcata tcggacttga tgctgacctt gacttcaatc
     3241 ttacgaagat cgtcgctgag gctgatatcc tgcactgttc cgacttcaac gccctgataa
     3301 cgaacaggcg tacggcccgg gacaatacca tccgccgaca taaagtcgat ggtgacggta
     3361 ttaccccggt cctgataact gtcccaaatc agccaaccgg caatcattag cgcgatgaaa
     3421 ggcagcagcc agaaaggtga gatacggcgt ttatttttaa tctgcgcttc agtcgtcgaa
     3481 gcgggcgttt cctgactcat gtgcatccca aagtaagcgg ctgtccagcc attccacagc
     3541 aagaatagtc aaaattacgg ctgcgccaaa ataaaacgca gccggtccca tagtaaaagc
     3601 gaggatctga tcgcgattaa tcagcgacat ggttaaagat atgacaaaca ggtcgagcat
     3661 cgaccagcgt ccaatccagg tcaccatccg cagtaacaga atgcgtgtgc gtaaaccttg
     3721 ctggcattta aaatgaatgc tcaacagtaa agtgaacatg acgatcactt tagtaaacgg
     3781 taccagaata ctggcgataa acacgattcc ggcgaccgca atgttgctac ttgccagcga
     3841 cataatcccg gaaagaatcg tatcttcctg ccggccacca ttcagataaa tgatagaaat
     3901 aggcaatagg ttggctggta acaacaaaac gattgacgct aacagcgccg cccagcattt
     3961 ttgcaaacta tgacgacgac gcaggcgtag cgggatatgg caacgcgggc agcgaccacg
     4021 ctgatcagga tagccggtaa aatggcaccc aagacagaca cgaagtttct cgtccctacg
     4081 cgtagcgggg cgctgcggat aatatcgctc ccacagctcc tcaacattaa gatgtgacaa
     4141 cgtcaccgtc gttaaaatca ccaacgccac aaaagagaac aagccgacac ccgcctggat
     4201 atgggcataa tcctgtacct ttatagaagc aacgccaatg ccgaccaggt agatatccag
     4261 cattacccac tctttaagac gctcaagcat cagcagtacc ggacgtaaat tcattcccag
     4321 tcggttacca aaccataaat atgctatgga ggacaccaga atgagggggg caccaataac
     4381 gcagaaaaag accatcgccc ccgttatcgt atcgccctgt ttggtcattt gccagatgcc
     4441 ttgcatgacg ttggcgtcga tacgaatacc taacagccag atatgcaaca gcggttcgcc
     4501 ccaggcaaac ggcatcagca acagcatggt gaaagccatt gccgccaggc gcgttagcga
     4561 ccagtcgcgc ccgtcgcgaa tttttgcctg acagcgcgga caataggcac tctgatgaga
     4621 atttatctcg ggcaggctaa acagcatgtc acattgcggg caacgttggt aatcaccacg
     4681 cggcagttcc tcgccgattg ccctcactgt tatctttttt gtcggcgtaa tttgtggtgt
     4741 gttaagagcc atgacgtgcc aaatgtaata agaattaaca ttaatcttaa cccatgatgg
     4801 gaatgatctt gagcattaac gcatttaatg cttattttaa taggctggca gttccaggta
     4861 agacaaacaa gtgattatat aatgaacaaa acagaatttt acgcggattt aaatcgcgac
     4921 tttaacgcgc tgatggcggg agaaaccagt tttctggcaa cgcttgcgaa caccagtgcg
     4981 ttgttatacg agcgtctcac tgacgtaaac tgggcaggtt tttatttgct tgaggacgat
     5041 acactggtac tcggaccatt tcagggcaaa attgcctgtg tccggatacc cgtcgggcgc
     5101 ggggtgtgcg gcactgcggt tgcccgcaat caagtgcagc gtatcgagga tgttcacgcg
     5161 tttgacgggc atattgcctg tgatgcggcg agtaattctg aaattgttct gccgctggtg
     5221 gtgaaaaatc agattattgg tgttcttgac atcgatagta ccgtcttcgg tcgctttaca
     5281 gacgaggacg agcaaggctt acgtcagctt gtggcacagc ttgaaaaagt gcttgcaacg
     5341 acggattaca aaaaattctt tgcgagcgtc gcaggataat caacggataa cgtagcattt
     5401 actggtagcg tcattataat gacgcctgtt catgcctgcg cttgttggct acgtccgttg
     5461 taatcaggaa atttcatgga aaatcaacct aagttgaata gcagtaaaga agtaatcgcg
     5521 tttctggccg aacgttttcc ccactgtttc agtgcggaag gtgaagcgcg tccgctgaaa
     5581 atcggtattt ttcaggattt ggtcgatcgt gttgccgggg aaatgaacct gagcaaaacg
     5641 caattacgat ccgctttacg tctctacact tcgagctggc gttatcttta cggtgttaaa
     5701 cccggcgcaa cgcgtgtcga tcttgacggc aacccatgcg gtgagctgga cgagcaacat
     5761 gtagagcatg ctcgcaagca gcttgaagaa gcgaaagcgc gtgttcaggc acagcgtgct
     5821 gaacagcaag cgaaaaaacg cgaagctgcc gcagctgctg gtgagaaaga agacgcactg
     5881 cgccgcgaac gcaagccacg tccgaccacg ccacgccgca aagaaggcgc tgaacgtaaa
     5941 cctcgtgcgc aaaagccggt agagaaagcg ccaaaaacag taaaagcacc tcgcgaagaa
     6001 cagcacaccc cggtttctga catttcagct ctgactgtcg gacaagccct gaaggtgaaa
     6061 gcgggtcaaa acgcgatgga tgccaccgta ttagaaatca ccaaagatgg cgtccgcgtc
     6121 cagctgaatt cgggtatgtc tttgattgtg cgcgcagaac acctggtgtt ctgaaacgga
     6181 ggccgggcca ggcatgaaca tgttttttag gcttaccgcg ttagctggcc tgcttgcaat
     6241 agcaggccag accttcgctg tagaagatat cacgcgtgct gatcaaattc cggtattaaa
     6301 ggaagagacg cagcatgcga cggtaagtga gcgcgtaacg tcgcgcttca cccgttctca
     6361 ttatcgccag ttcgacctcg atcaggcatt ttcggccaaa atctttgacc gctacctgaa
     6421 tctgctcgat tacagccaca acgtgctgct ggcaagcgat gttgaacagt tcgcgaaaaa
     6481 gaaaaccgag ttaggcgatg aactgcgttc aggcaaactc gacgttttct acgatctcta
     6541 caatctggcg caaaagcgcc gttttgagcg ttaccagtac gctttgtcgg tactggaaaa
     6601 gccgatggat ttcaccggca acgacactta taaccttgac cgcagcaaag cgccctggcc
     6661 gaaaaacgag gctgagttga acgcgctgtg ggacagtaaa gtcaaattcg acgagttaag
     6721 cctgaagctg gcaggaaaaa cggataaaga aattcgtgaa accctgacac gccgctacaa
     6781 atttgccatt cgtcgtctgg cgcaaaccaa cagcgaagat gttttctcgc tggcaatgac
     6841 ggcgtttgcg cgtgaaatcg acccgcatac caactatctt tccccgcgta ataccgaaca
     6901 gttcaacact gaaatgagtt tgtcgctgga aggtattggc gcagtgctgc aaatggatga
     6961 tgactacacc gttatcaatt cgatggtggc aggtggtccg gcagcgaaga gtaaagctat
     7021 cagcgttggt gacaaaattg tcggtgttgg tcaaacaggc aagccgatgg ttgacgtgat
     7081 tggctggcgt cttgatgatg tggttgcctt aattaaaggg ccgaagggca gtaaagttcg
     7141 tctggaaatt ttacctgctg gtaaagggac caagacccgc actgtaacat tgacccgtga
     7201 acgtattcgt ctcgaagacc gcgcggttaa aatgtcggtg aagaccgtcg gtaaagagaa
     7261 agtcggcgtg ctggatattc cgggcttcta tgtgggtttg acagacgatg tcaaagttca
     7321 actgcagaaa ctggaaaaac agaatgtcag cagtgtgatc atcgacctgc gtagcaatgg
     7381 cggtggggcg ttgaccgaag cggtatcgct ctcaggtctg tttattcctg cgggtcccat
     7441 tgttcaggtc cgcgataaca acggcaaggt tcgtgaagac agcgataccg acgggcaggt
     7501 gttctataaa ggcccgctgg tggtactggt tgaccgtttc agtgcttcgg cttcagaaat
     7561 ctttgccgcg gcaatgcagg attacggtcg tgcgctggtt gtgggtgaac cgacgtttgg
     7621 taaaggcacc gttcagcaat accgttcatt gaaccgtatt tacgatcaga tgttacgtcc
     7681 tgaatggcca gcgctgggtt ctgtgcagta cacgatccag aaattctatc gcgttaacgg
     7741 cggcagtacg caacgtaaag gcgtaacgcc agacatcatc atgccgacgg gtaatgaaga
     7801 aacggaaacg ggtgagaaat tcgaagataa cgcgctgccg tgggatagca ttgatgccgc
     7861 gacttatgtg aaatcaggcg atttaacggc ctttgggccg gagctgctga aggaacataa
     7921 tgcgcgtatc gcgaaagatc ctgagttcca gaacatcatg aaggatatcg cgcgcttcaa
     7981 cgctatgagg gacaagcgca atatcgtttc tctgaactac gctgtgcgtg agaaagagaa
     8041 taatgaagat gatgcgacgc gtctggcgcg tttgaacgaa cgctttaaac gcgaaggtaa
     8101 accggagttg aagaagctgg atgatctacc gaaagattac caggagccgg atccttatct
     8161 ggatgagacg gtgaatatcg cactcgatct ggcgaagctt gaaaaagcca gacccgcgga
     8221 acaacccgct cccgtcaagt aatatcaatc aggcacaaga aattgtgcct gattttttaa
     8281 cagcggcaag atgccgtaaa tcagatgcta caaaatgtaa agttgtgtct ttctggtgac
     8341 ttacgcacta tccagacttg aaaatagtcg cgtaacccat acgatgtggg tatcgcatat
     8401 tgcgttttgt taaactgagg taaaaagaaa attatgatgc gaatcgcgct cttcctgcta
     8461 acgaacctgg ccgtaatggt cgttttcggg ctggtactga gcctgacagg gatacagtcg
     8521 agcagcgttc aggggctgat gatcatggcc ttgctgttcg gttttggtgg ttccttcgtt
     8581 tcgcttctga tgtccaaatg gatggcatta cgatctgttg gcggggaagt gatcgagcaa
     8641 ccgcgtaacg aaagggaacg ttggctggtc aatactgtag caacccaggc tcgtcaggca
     8701 gggatcgcta tgccgcaagt ggctatctac catgcgccgg acatcaacgc ttttgcaacc
     8761 ggtgcgcgcc gtgatgcctc tctggttgct gtcagcaccg gtttgctgca gaacatgagc
     8821 ccggatgaag ccgaggcggt aattgctcac gaaatcagcc acatcgccaa tggtgatatg
     8881 gtcaccatga cgctgattca gggcgtggtg aacaccttcg ttatctttat ttcccgtatt
     8941 ctggcgcagc ttgccgcggg ttttatgggc ggaaatcgtg atgaaggtga agagagcaac
     9001 ggcaacccgc tgatctactt tgcggttgca acggttctgg aactggtgtt tggtattctg
     9061 gcgagcatta tcaccatgtg gttctcgcgt catcgtgaat tccatgctga tgccggttcg
     9121 gcaaaactgg ttggtcgcga gaaaatgatt gccgcgctgc agcgcctgaa aaccagctat
     9181 gaaccgcaag aagcaaccag catgatggct ttctgcatta acggtaagtc gaaatcgctc
     9241 agtgagttgt tcatgactca cccgccgctg gataaacgaa ttgaagctct gcgtacgggt
     9301 gaatacctga agtaatatga agtaaatact aaaaagcgcg tcctgatcga cgcgcttttt
     9361 ttatgccttg gatcgtggct gagtgatacg taaaccactg acacaggcag caatcactgc
     9421 cagaatagcc gcagccatca gcgaaacgtg cgtaccatta tcaccgaact gatttagcat
     9481 cagcgccacc agtgccgcgc cgctactctg acccagtaga cgagccgttc ctaacatgcc
     9541 actggctcca ccgctacgtt cgcgaggagc ggaggtaata atggtgtggt tattgggtga
     9601 ctggaacaag ccaaatccag cgccacataa gatcatcggc cagataatat tgatatccgc
     9661 aggtgacgcg ggcagcagaa ccaggggaaa aagccccgca gccatgatga acaaccctag
     9721 agcacccagc aatcctgcat gtacgcgttc aatcaaatag cctgccaacg gagccatcac
     9781 catcgttgct aacggccacg gtgtcagaag taaacctgtt tcgacttcac tacgcccgag
     9841 cacggtttgc aggtaaaagg gcagggaaac cattgccagc atttgtgcgc agaaagagca
     9901 aacagatgtg caaatagaaa gtgaaaacag cgggatacgc agtaaatcca ccggtagcag
     9961 cggtacggga agagaaagct ggcggcgaat aaagaaaata ccaacaacaa ccattaccac
    10021 cagttccgca gcaattaacg tcagcgattg cccctgagcg aaaccactca acgcagtgat
    10081 aagcaggccg aaggttaacg cgttcatcac ggcgctgggc aggtcgaaac ggggtttact
    10141 ggcgcgagaa ccattgggtg gcagaaaacg catcgccaga agcagggcga taatacctaa
    10201 cggtacgttg attaaaaata accatttcca ggatgcgatg gagaggattg ctgcagcaat
    10261 tgtcggcccg gcagcagaag agacggcaac aatatggcga ttgttggcag ggcgacgttg
    10321 gcgattgcgc cgtcaaggac agccatcgaa atgccaatca caatggttaa tatcgcaccg
    10381 tatcgctggg gcaatggcag gccgtcggcc tgaacttttg gcataggaat tttatatctt
    10441 tggtgaataa tcaacagggg tatgattcta gcactgatat tcacggtgga tgtcgcagaa
    10501 ttgtaactaa ccggcaaaag atggattgcg accaccctgg tgcgaaatta taataaaaaa
    10561 cagttctgat ttttataaaa cactcgcaat gaggtgataa atggctaacg cagatctgga
    10621 taaacagcct gattctgtat cttccgtgct aaaagttttt ggcattttgc aggcgctggg
    10681 tgaagagcgc gaaataggga taaccgagct gtcgcagcgc gtcatgatgt caaaaagcac
    10741 cgtttatcgc tttttacaga ccatgaaaac cttaggttat gtggcgcagg aaggggagtc
    10801 ggagaaatat tccctgaccc tgaaattgtt tgaactgggc gctcgcgcgt tacaaaacgt
    10861 cgatttaatt cgtagcgcag atatccagat gcgtgagatc tcccgcctga ccaaagaaac
    10921 tatccacctc ggcgcactgg acgaagacag tattgtttac attcacaaaa ttgactctat
    10981 gtacaatttg cgcatgtatt cacggattgg gcgtcgtaat ccgctgtaca gcaccgcgat
    11041 tggtaaggta ctgctggcat ggcgcgatct cgatgaagtg aagcaaattc ttgagggcgt
    11101 ggagtataaa cgcagtaccg agcggaccat caccagtaca gaagcgttat tacccgttct
    11161 ggaccaggtg cgcgagcagg ggtatggcga agataacgaa gagcaggaag aagggctgcg
    11221 atgcattgcg gtaccggtat ttgatcgctt tggcgtggtc attgccggtt tgagcatctc
    11281 cttcccgacg ttgcgtttct ctgaagagcg tttacaggaa tatgtcgcaa tgttgcatac
    11341 cgcagcgcgc aaaatttctg cccaaatggg ttatcacgac tatccgttct gatgagagta
    11401 agaacctgtc ggaatatcaa acagacaggt tctttattta gcatgagaaa aataaagttg
    11461 aaggtgcgtt atattaaacg cgcttgctat aagaatattt tactcatgag tgagaatctg
    11521 gttgtttatt acccttaacc attatcgacc acgatattgc ttttacgtaa cagtgggcaa
    11581 tctgttatcc ccaagaaacc acttttagtg tgcaagtatt gtaccgtgct ggtgcctctg
    11641 gcagtcagat aggtacattg caaacctaat cctgcggcat tctctttgct tccaatcaaa
    11701 acgccatatc cgctgagtaa taatcctatc cataccagtg ctatcagcat aactgtgcga
    11761 atgatgaatc gcattacaac ctcttctctt tttatgttcg cttaatcgta gcggcaatat
    11821 gcgctgaagc aagctactca ttccgaaaaa gcacgaatat cgacatagtt aggcgctgtt
    11881 taactaacgc atgctagttt aatgacataa ggtaggtgaa gcggagattg gagtgaaaaa
    11941 gtttcgatgg gtcgttctgg ttgtcgtggt gttggcttgc ttgctgcttt gggcgcaggt
    12001 attcaacatg atgtgcgatc aggatgtaca atttttcagc ggaatttgtg ccattaacca
    12061 gtttatcccg tggtgatatc attttttcag cactagattt tcttcctctc cagcagtggt
    12121 agaatagcgc ctctactcag aggtggtgaa atgaacgaag ttgtaaattc aggcgtgatg
    12181 aacattgcgt ctttggttgt atcggtggtg gttcttctta tcgggctcat cttgtggttt
    12241 tttatcaatc gtgccagttc acggactaac gaacagattg aactgcttga ggcgttgctg
    12301 gatcagcaaa aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa cgaaccagag
    12361 aaagcagata aaaagaccat tgagagtcaa aaatcggttg atgacgaaga tattattcgc
    12421 ctggtcgccg aacgataatc cttcctcaat cttgagtaac cccgtcgttg aagggttact
    12481 caattcatcc tgattatacg cactgtcatg gtgcaactgc ttcaaattaa aactctggtc
    12541 tgacttcaaa taattatcat attatttatt tgcgtaacgg gatatcagaa aaagatattt
    12601 tcctcattaa tttattagga tgtttacatc ggatttgtga ttaagcgtgg tattatttat
    12661 tagcgaaacg tttctctctt gatttttttg ctcattcatc aatttttctt attttaaatt
    12721 ttcaatccct ttggggattg acttctcttt agggtaattg atagccgtta accgactgtt
    12781 ttatgagaaa aagtgatata actttttatt cattgcatag caaaaaatgt gatattgcac
    12841 gcactatgta ataacttctc ccactggcct ggaacaactg aacttattga actatgttag
    12901 aaaatacgcc agtttaagta tctgcctgaa ctggcaaggt taagcacaat gatatatcgg
    12961 cgcgtattcc gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc
    13021 tgatcgagac atgtttaaaa atggcttgcc ataattaacg ttgtatgtga taacagattt
    13081 cgggttaaac gaggtacagt tctgtttatg tgtggcattt tcagtaaaga agtcctgagt
    13141 aaacacgttg acgttgaata ccgcttctct gccgagcctt atattggtgc ctcatgcagt
    13201 aatgtgtcag ttttatctat gttatgcctg cgggcgaaga aaacaatcta aggaattttt
    13261 caaatggcaa agattaaagg tcaggttaag tggttcaacg agtctaaagg ttttggcttc
    13321 attactccgg ctgatggcag caaagacgtg ttcgtacact tctccgctat ccagggtaat
    13381 ggcttcaaaa ctctggctga aggtcagaac gttgagttcg aaattcagga cggccagaaa
    13441 ggtccggcag ctgttaacgt aacagctatc tgatcgaatc cactgatctg aagtgtgaat
    13501 acgcttcaat ctcgctataa agcctcgtcg aatgcgaggc tttttactat gctttatctt
    13561 cgctcctggc gttcggatat ttgcccgccg cgtgattcgc gttacacttg cggcctttag
    13621 tatcctgccg gagttgtcat gtctttttcc tgtccacttt gccatcagcc tctttcgcgt
    13681 gaaaaaaaca gctatatctg tccccagcga catcagtttg atatggcgaa agaagggtat
    13741 gtcaatctgc tgtccgttca gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg
    13801 atgcaagcac gccgcgcatt cttagatgcc ggacattatc agccgctgcg tgatgcaatt
    13861 gtcgcccaac tgagggaacg gcttgatgat aaggccacgg cggtgctgga tattggctgt
    13921 ggtgaagggt attacacaca cgcatttgcc gatgcgttgc ccgaaatcac cacgtttggt
    13981 ctggatgttt cgaaggtagc gataaaagcg gcggcgaaac gctatccgca ggtcactttt
    14041 tgtgtcgctt ccagccaccg tttgccgttt tccgatacca gtatggacgc cataatacgt
    14101 atttacgcgc cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg cggctgggtc
    14161 attactgcca cgccgggacc gcgacatttg atggagctga aggggctgat ttacaatgaa
    14221 gtacatcttc atgcacctca tgcagaacaa ctggaaggtt ttacattaca gcagagtgcg
    14281 gagttgtgtt atccgatgcg tcttcgcggt gatgaagccg tcgcattatt gcagatgacg
    14341 ccgtttacct ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa agaagtgttc
    14401 gactgccaga cggactttaa tattcacctc tggcagcgtt cttattaacc gtggaagtgc
    14461 gtccagagga tctggacgcc gatgccgatc agtaccagcc cgccgagaat ttctgctttt
    14521 ttcccaataa ttgagccgat aaagcgacca accatcatcc ctaatgttga cataatcaag
    14581 gttgcacaac caatggccaa tgcggtcgcg ataatgttga cctgcaggaa agcaagacca
    14641 acacccacag ccatggcatc caggctggtg gcaatcgcgg tggttaccag tagccagaaa
    14701 ccgtgtcgac ggcgcggctc ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc
    14761 attcgcccgc cgaggaatat cagcagcaca aacgcaatcc agtggttcca ttcaaggaca
    14821 aaccggctgg ctaacatgcc cattccccag ccgatcagcg gcgtcagggt ttcgacggca
    14881 ccaaaaataa ggccggttcg caatgcttca gaaaatttcg gtttatggag ggtggcacct
    14941 ttaccgattg atgcagcaaa tgcatccatc gacataccaa acgcaagaag aacagtagca
    15001 gtgatattca tgacaatgtc ctgaccgggg tatccataaa acacatcatc gcccccagca
    15061 aacatgcata catgaacaac aagccgtgat ggttttcgcg ttcatgcagg catagcgatt
    15121 gatgtgcata tggtctcgcc tgactgaaag tattcagtcc gtacgtgcca cgttttgcaa
    15181 cgagtatgtt gacacgtaca tttccggaat ctggaaatcg gctactcccc aaatgagggc
    15241 gcaaccttaa catattttga gaatataaaa caactgttgg aatatattat ttcattgctt
    15301 tattgataac gattttcatt tggatttata tatcaatgaa acgttaaaat aaataacaaa
    15361 taagggaaga ggtaatatag cctaagctat gtctgattat taaaaataca ctaaacatag
    15421 ctttggctaa attcataact tattgagttg aaacgagaag cttataaatt ttttccagat
    15481 cgtcgatatt tcgaacgcgg attaacagcc gacgctgttc taattgcatc accaacacgc
    15541 catcttccga caagttcatc gctttgattc ggctatattc aatccagaca ttggcgaaga
    15601 aaaaaccttt ttgtttaaag atgatcttcg gaacgcggat ccagaatata taaaaaccca
    15661 tcagagccag tgcgcttaat aaccatgtcg ttattaacgc accatgattc gtgacgttgt
    15721 tatagataag aatgacaatc aatccgacga agataacgct atcgatgcga ccacgccgga
    15781 gcaaaggaat tgccagcagg gtggggccgt tacggcgggg catgatgaac tgatcgtaga
    15841 tcgcgaaggc caggagtgcg gcgatgaaaa gaatcagtac caggtccgtg attgtcatta
    15901 atcctccaga taaaaaaacg gggccaaaag gccccggtag tgtacaacag tcttacagtc
    15961 ccagcaggcc gcaagcgtaa ccagcgatac cgatgacgaa gaagccaacg atgatccaca
    16021 gcgggttaac ttttttgcgc agtagccaca tacaagcaaa ggtcagcagc agtggtacca
    16081 ggcctggcat taactggtcc agaatagtct ggacagtagt aacgtgttct ttgcccgtct
    16141 ggtcagtaat gcgagagaca accagcggga tgttgacatg tgtccacttg ttaaccaatg
    16201 cccccatgac aaacaggccg aggatagacg ccccttccgt cagtttttgc aggaagccac
    16261 cacccatatc tttaacgata tcgatacctt tggagtaacc atacgctacg ccgtagtaac
    16321 gggttgccag acgcaccagg ttaaacagga tgaagaacag cagcggacct aacaggctgc
    16381 cgctcatcgc gataccggca cccagtgctg caaataccgg acgtacggtt ccccagaaga
    16441 tcgggtcgcc tacaccagcc agcggcccca tcaaaccgac tttgataccg ttgatagcac
    16501 cgtcgtcgat ctctgcgcca ttagcacgct gttcttccag cgccagggtt acgccgagaa
    16561 tcggcgcagc cacgaacggc tgggtgttaa agaactccag gtgacggcga atagcttgtt
    16621 tacgagcttc gttgttctca gggtagaggc gacgaattgc cggtaccata gagaagcaga
    16681 aacccagtgc ctgcatacgt tcgaagttcc atgaaccctg gaagaggtta gaacgcagga
    16741 agacgccacg aatatcactt tgagtgagtt ttttctcggt ggtagtttga gttgtatcaa
    16801 ccatttcgct cacctgttag tccagttcgt tatcgagatc gttgttacca gctgcctgag
    16861 caggcgcacc ggctacgcgg ttgtatttcg ggctaagttg gatgtagagc actgccataa
    16921 cagtaccaat cacacccaga gcaaccaggt taaagttggt gaatgctgcg gttacgaagc
    16981 cgaggtagaa gaacggcatc aggtagccag cacgcatcat gttgataacc atcgcataac
    17041 caaccaccac gatcatgcca ccggcgatat tcagaccatt ggtcaccact tccggaatcg
    17101 cattcagcat gttctgtact tcgctggtac caacagacag cgcaacgatg acggccggaa
    17161 tagccacacg cattgcttgc aggaacagag aagaaacgtg gatccaggaa atcgctgtca
    17221 ggttgccgtt atcagcagcc ttatccgcag cgtgctggaa agcaacggta atagtacgaa
    17281 cgatgatggt cagtacctgg cccgcagcgg ccagagggat tgccagtgcg atacctgcac
    17341 caatgctctg atgacctgcg ataaccagaa tggtagaaat gatagaagcc agagcggcgt
    17401 caggcgcaac tgcagcaccg atgttcatcc agcccagcgc gatcatttcc agcgtaccac
    17461 cgataataat accggttttc atatccccaa gaacgatacc caccagggta cacgcgatta
    17521 gcggacggtg aaactgaaat tcatcgagga ttgatcccat acctgcgata caggctacga
    17581 taaataccag cacaatttga agagtggtaa tctccattgt acttctcctg tttacgtaag
    17641 acttaagtgt gaaaactgag tgataatcaa cacaatacgt tacttatcga ttttgctgat
    17701 cagatccatc attttcagtt tcggatcggt ggaaacctta cggacttcca gctcaatacc
    17761 gcgcgcattc agtttcttga acgcctcgat atctttttca tcaaccgaaa ccgcgttatt
    17821 cacctgggtt ttaccctgac ggaatgccat accaccgacg ttaacagagg tgattttcac
    17881 gccgccttca acgagacgct ctacatctgt tgggttggta aataacagca ttacgcgttc
    17941 gccagcatat ttcgggttgt tgtagacgcg aatcattttg gcaacatcaa ctacgtgtgc
    18001 tgttacgccc ggaggtgcaa cctgggtgag cagtgtctta cgaacggtat ccgcagccac
    18061 ttcatcacta acaacaataa tacgggagac attggtttct ttggtccagc gggtggcgac
    18121 ctgaccgtga atcagacggt cgtcgatacg cgcaaggcca ataaccatgt agtcgtttgg
    18181 ccccattggt tttgccggag ttggagccgc ttttggtgct gcggcagcgg gtgctggcgc
    18241 ggctttttca accggtttgg ctttcagtgc tttcacgcct tcacggcctg tttctactgc
    18301 cagcgcaacc agttcatcaa agcttgggtc atcatcacga gccattaacg tttccacgag
    18361 cattggaatg ttaacgcctg caatgacttc ataatgctct ttgtcgacga caatgcggct
    18421 ggcagcattg aacgggctgc ctccccatgt atcaacgaga aacagcacgc ctttagtggt
    18481 gtcgagtttt gccaactgag cgttgtactt ttcaatcagc gtttcggcat tttcacctgg
    18541 aacgaaatcg atccagccga cgttttcctg ctcgcctaac agcatttctg ccgttttaag
    18601 caactgctct gcagcccaac catgtgtgcc tataacaata gcaatggtca cttgctacct
    18661 cctttattat tgttaacacc tcaacgtgcc agatgtattt ttgaatcgtc gtccacaatc
    18721 gaatcgattc agataagggc tacaaccaaa aacccctgcg tttcgcgatt tattttagat
    18781 atcgaaaaaa ttattttatg tgatgaagat ccgtaattta actttcgatt agcagaaatt
    18841 tcgaaaggta aaatatcctt gtcacattcg tttgcaaagg aaggtaaatc tttgccaaat
    18901 cagaggcgtc tctgatatgt ttaactcccg tttaacaacc atggagtata gggcagtagc
    18961 ccgcagtatg gatcgtcacc gacgtcattt cagcatcagg ccttttaacg cctgcctttc
    19021 tggcactcta tgccgcacct ttcgtttgca ttttgtcgtt acgcctgcat tatttctggc
    19081 gtcgaatagc tattccttaa gcaggagctt gtcatggaat tcttaatgga cccctcaatt
    19141 tgggcggggc tactcacgct tgttgttctc gaaattgtgc tgggtatcga taacctggtc
    19201 ttcatcgcca ttcttgctga caaactgccg ccaaaacaac gcgataaagc gcgtttgctg
    19261 gggttatcac tggcgctgat tatgcgtctg gggctgctgt cgctgatttc atggatggtc
    19321 acgctgacca aaccgctatt taccgtcatg gatttctcct tctccggacg cgacctgatt
    19381 atgttgttcg gggggatatt cttgctgttc aaagcaacaa ccgaactgca tgaacggctg
    19441 gaaaaccgcg atcatgattc cggccacggt aaaggctacg ccagtttctg ggtggtcgtc
    19501 acacagattg tcatccttga cgccgtcttc tcgttggatg cggtaattac tgcagtaggg
    19561 atggttaacc atctgccggt gatgatggcg gcggtagtga ttgcgatggc ggttatgttg
    19621 ctggcatcca aaccgctgac gcgattcgtt aaccagcacc ccacggtggt ggtgctctgt
    19681 ctgagcttcc tgttaatgat tggtctgagt ctggtggcag aaggtttcgg tttccacatt
    19741 ccgaaaggtt acctgtatgc cgcgattggc ttctcgatca tcatcgaagt gtttaaccag
    19801 attgcgcgtc gcaactttat tcgccaccag tcgactttgc cgctgcgagc gcgtactgcc
    19861 gatgccatcc tgcgtttgat gggcgggaaa cgtcaggcca atgttcagca cgatgccgat
    19921 aacccgatgc cgatcccgga aggtgcattt gccgaagaag aacgttacat gattaacggc
    19981 gtactgacgc ttgcgtcgcg ttctctgcgc gggatcatga cgccgcgcgg tgaaataagc
    20041 tgggttgacg ctaatctcgg ggtcgatgaa atccgcgagc aactgctctc ttcaccgcac
    20101 agtctgttcc cggtatgtcg cggtgaactg gatgaaatca tcggtattgt acgtgctaaa
    20161 gaactgctgg tggcgctgga agagggcgtt gatgtggcgg cgattgcttc ggcgtctccg
    20221 gcgattatcg tcccggaaac cctcgatccg atcaacctgt tgggcgtgct gcgtcgtgct
    20281 cgcgggagct ttgttatcgt gaccaacgag tttggtgtgg tacaaggtct ggtcacgccg
    20341 ctggatgtgc tggaagccat tgcgggtgaa ttcccggacg ctgacgaaac gccggaaatc
    20401 attactgatg gtgacggctg gctggtaaaa ggcggtacag atttgcatgc cttgcagcag
    20461 gcgcttgatg ttgagcacct tgccgatgac gatgatatcg cgacggtcgc gggcctcgtg
    20521 atctcggcaa atggtcacat tccccgtgtg ggcgatgtga ttgatgtagg gccactgcat
    20581 atcaccatca ttgaagccaa tgattatcgt gttgatctgg ttcgcattgt taaagagcaa
    20641 ccggcgcacg atgaagatga gtaagcatta acgtaacggc ataatgggcg tgatatgtcc
    20701 attatgccgg gcgggcggcg gttggctgcc cgccagccat ttgggaaaat cacgtagcgg
    20761 catcggctgt gcgtacaaat acccttgcaa aatatgcacg ccatgacggc gcaaatactt
    20821 cgcctgttct tgcgtttcca cgccctccgc caccagttca atattcagtc tttgccccag
    20881 cgcgatgatg atatcggtca ccgtcgagtt aaccgcgtca gaacctatag ctgcggtaaa
    20941 tgacttatca attttcagca tgtcaggacg taatgtttca agccaggaaa atgaactgtt
    21001 gccggtgccg aagtcatcaa tcgccagttt gacgttttta cgatgtagct cgcgggcaat
    21061 ccgataatca acatccagta aggcatcgcg ttcggtgatt tcgaggatca gttgctgaat
    21121 cgggtgagcg ctaaaccagt actgattgag atcttttatc agcacaccac ggcgaaaatg
    21181 gctgggtgcg acgttaatgc caacatgaaa ctgactactc atcgggaaaa catggcgctg
    21241 acgaatggtt tctgccatca catagcgggt cagtggcaca attaaatgat gttcttccgc
    21301 gataggaata aacacatccg gtgaaatcca gccctgacgc ggattgttcc agcgcagcaa
    21361 aatctctaca ccaatacatt gctggctgcg cgcattaagc aaaggctgac agaacaattc
    21421 gaactcatgt tgcgccaggc ccagattgat ttcgcgggaa aagctcatcc ggtaagcggt
    21481 cgccagccag gcgatgtagc ccaccagtaa acttagcagc accgccagcg gtaattgtgt
    21541 tggaagatag tgccatgcca gcgccgtagc ccccggacca ttaacgttaa tggtaaaagg
    21601 aaagcgttgc gaagaaacct gatagttttc attgtcttca ggttgcggaa gggaatctac
    21661 cagcccatta ccatagagca aatgccgttt gtccaccgtc aggctggcgc tactgatttg
    21721 cggcagttgt ggctcaagga gcattgccgt cagtaagtcg atgttaatca tctccatgac
    21781 cccagcattg ctactgcccg ctgcaggcgt ccattgaatc aaaaccggac tgcctttaat
    21841 cagggcacgg tcgatcgtca ggcgtaaaag tggttgcggt gcaggaagtt cagccagaat
    21901 gtccacgacg ggcacattgc gataaccaaa aatgctggag caatagagtg ttccgtcttg
    21961 caccaggcca atggatcgca cagtttggag ttttgccgcc tgtttacgta atggcaaatg
    22021 ggcgacagag cagggcagac caatcagcgg aagcaagact tcgctacctg cctgtagggg
    22081 aagcagtact ttatctaatt cctctacagc gtgattggcg aattgtacta cccgttgttg
    22141 atttaagtta cgctgtgaaa taaatcgcac gctcagggtc gaagcgagcg taacgagtgc
    22201 gcaaatcgta caaacaatca ttcgattacg gcgataggtt ttaatgatcc gttgtgcttt
    22261 ttgcatgcgg gccaccctgt aagcgtatgg cgacaaacgc ctgacgcaac agtggaagtg
    22321 tagtggggaa tattacagca gacgagaaag cgggtataaa ttcgcccatc cgttgcagat
    22381 gggcgagtaa gaagtattag tcacactgga ctttgattgc cagaccaccg cgtgaggttt
    22441 cgcggtattt ggcgttcatg tccttaccag tttcgtacat cgtttcgatg accttatcca
    22501 gcgagacgcg cggtgcactg gtgcggcgca gagccatccg tgcggcgtta atcgccttca
    22561 cagaggcaat ggcattacgc tcaatgcacg gcacctgaac ctgccctgca accgggtcgc
    22621 aggttaaacc aaggttgtgt tccatgccaa tttccgccgc cacgcaaacc tgttccgggc
    22681 taccgcccag cagttctgca agacccgcag cagccattga acaggcaaca cccacttcgc
    22741 cctggcaacc aacttccgca ccggaaatag aggcgttcat tttatacaat gcaccaatcg
    22801 cgcccgctgc cataaagtaa cgggtataga tgtccgggct gaccgattca ataaagtggt
    22861 catagtaagc cagcactgcc ggaacgatac cgcaggcacc gttggttggc gcagttacca
    22921 cacgaccacc ggcggcgttt tcttcgttaa ctgccagcgc aaacatgttt acccagtcaa
    22981 tgacattcat cggatcgtta gacagtttat cgctggaaac taacatccgg cgcagggcag
    23041 acgcacgacg tggcacgcgc agcgggcctg gcagtacacc ttcggtgttc atcccgcgat
    23101 cgatacatgc ctgcatggtt tgccagacat gcgcgaaata ctcgtcgatc tctttcttgc
    23161 tgtgcagcgc cagttcgttc tgcatagcga gaccagacag cgaatagccg gtttcattac
    23221 agtacgcgag cagttcggtg gcagatttga acggatacgg cacgctcact tcgttggcag
    23281 catcctgacc aaagtgttct tcatcgacga taaaaccgcc gccgatggaa taataagttt
    23341 tgctgtagac gacttcatcg ccgttatagg cgtggatttg cataccgttt tcatgcagcg
    23401 gcaggttgcc gttatgaaaa cgcatcccgt tgtcgcgcgg gaaatccact tcatgccgtc
    23461 cctgtgccag cagcagacgt tcgcgctctt ctacgtcgcg aataaaaccg ggaatactgt
    23521 cgatatccac ggtggcaggt tcgttacctg caagacccat aataatggcg atatcggtgt
    23581 ggtggccttt acccgtcagc gacagtgaac cataaacgtc cacggcaacg cgagtaacgc
    23641 tatccagtaa gcctttttcg accagatcat cgacgaactg tttacctgcc ttcataggcc
    23701 ctacggtatg ggaagatgag ggaccaatcc ccaccttaaa catgtcgaat agactaatca
    23761 cgataatact cctgacaagg tgactggact tccagtaacg aactaataac agcgcatagt
    23821 gtaagaggga acgcggcgac tggcttaact attcacatga attaaactaa tcaaaaaccg
    23881 cgatttaact aatattgcga tgattgtctc aagtagtgtt ttgtagatgt aaagataagt
    23941 atagtcaggg tttcacacca atttgcagcg ccagctcacg aattatgcct gcggtcattc
    24001 cccatacaaa atactgttcg taccaggaca gccatacccg atgtgaatcg ccacggcggt
    24061 agatatctaa agggtgataa cgacccagat gtaatgcctg ggcgagcggc atttcaaaca
    24121 ccgccgagac ttcatcttca ctggcgcgat acggcagatc gggcgggata atgccgacca
    24181 ctggggttac ctgatagcca gttacgctat cgacgggcgg cagcacgccg ataacttcaa
    24241 cggcggaagg cggtatagcg acctcttctt cagcttcgcg cagcgcggcg gcgataactg
    24301 atgcgtccgt gtcatcgact gcacctccag ggaatgccac ttgtccagcg tgtttacgca
    24361 gatgaattga acgctgagtc agcaacaacc ccggttgcgg tcgacggacg atggggatta
    24421 acacggcagc ctgacgatga tttagggttt cccggttaat ttgcgggcgc aaaagttgaa
    24481 agcgcgataa aaaatcatca agcatcaggc tacggtattc cacgtcttac ttctccagtt
    24541 ggcgtaatat cttattaact ttatcaaaag tttcctgata ttccgcttct tcctggctat
    24601 cggcgacaat tccaccgccc gcagagcagt atatttgtcc gttaatggca gtcagagtgc
    24661 ggatagtaat gctggtatcc atgttgccgc aaaagctcaa atagccaatg ctgccgcacc
    24721 aggcattacg tcgctgcggt tccagttcgt cgataatttc catagcccgt actttcggag
    24781 ccccggttat tgagccacca ggaaaagctg cgcgcagcag atcgctggcg tgtaactgtt
    24841 ctggtagttg tgccgttatg gtgctgacca gatgatgcac ggcagggaag ggttccacca
    24901 cgaacagctc tggtactttt accgaacctg ctacggcaac acgaccgata tcattacgca
    24961 ttaaatcgac aatcatcaga ttttcggcac gatctttcgc tgagttcgcc agtttttctg
    25021 cttgtttgct atcttcctga ggatcgggca ggcgtggtag cgtgccttta atagggcggg
    25081 tctggatttc actattatca caaagaataa accgctctgg cgaaaggctt aaaattgcac
    25141 cctgttcaag acgtaaaaaa gcgctaaatg gcgcgcggtt ggcctgatta agctgaagga
    25201 atgcctgcca ttcatcgcca gaataggtcg catgaaaacg ctgggcgaga tttacctgat
    25261 agcaatcacc gctgtgcaga tattcctgta cctggcgaaa tttttcgccg tactgctcgc
    25321 gggtcatatt ggattgccag tcggaagtaa gcgtgaaatc ttcctgcggc gagaattgct
    25381 ggctttccag ccaggcccga cgggcattga catcattatg actcagcaaa gaaactgtat
    25441 gacgctggtg gtcgacaatg agcgcccaat cgtagatacc cactgccata tccggcagaa
    25501 cgatatcttg ttccgcaatt tctggcagtg actcaaaacg gcggcccaga tcgtagccaa
    25561 acaaccccag tgcgccg
//
LOCUS       DAJVRF010000050        25493 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJVRF010000050 DAJVRF010000000
VERSION     DAJVRF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25493
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.050"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            14..484
                     /locus_tag="OQN13_001848"
     CDS             14..484
                     /locus_tag="OQN13_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCS1615221.1"
                     /translation="MVDQNLPWSASLKVGDKKLPVVVVRVGEASVDVLVGQQTWTLTH
                     KWFESVWTGDYLLLWKMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPL
                     LVEKIKQFQKSHHLKTDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            486..905
                     /gene="gspB"
                     /locus_tag="OQN13_001849"
     CDS             486..905
                     /gene="gspB"
                     /locus_tag="OQN13_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HCS1615222.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVKESDEKAGLRERVKNALNELER"
     gene            1143..1454
                     /gene="rpsJ"
                     /locus_tag="OQN13_001850"
     CDS             1143..1454
                     /gene="rpsJ"
                     /locus_tag="OQN13_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCS1615223.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1487..2116
                     /gene="rplC"
                     /locus_tag="OQN13_001851"
     CDS             1487..2116
                     /gene="rplC"
                     /locus_tag="OQN13_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCS1615224.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2127..2732
                     /gene="rplD"
                     /locus_tag="OQN13_001852"
     CDS             2127..2732
                     /gene="rplD"
                     /locus_tag="OQN13_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCS1615225.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2729..3031
                     /gene="rplW"
                     /locus_tag="OQN13_001853"
     CDS             2729..3031
                     /gene="rplW"
                     /locus_tag="OQN13_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCS1615226.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3049..3870
                     /gene="rplB"
                     /locus_tag="OQN13_001854"
     CDS             3049..3870
                     /gene="rplB"
                     /locus_tag="OQN13_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCS1615227.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3887..4165
                     /gene="rpsS"
                     /locus_tag="OQN13_001855"
     CDS             3887..4165
                     /gene="rpsS"
                     /locus_tag="OQN13_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCS1615228.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4180..4512
                     /gene="rplV"
                     /locus_tag="OQN13_001856"
     CDS             4180..4512
                     /gene="rplV"
                     /locus_tag="OQN13_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCS1615229.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4530..5231
                     /gene="rpsC"
                     /locus_tag="OQN13_001857"
     CDS             4530..5231
                     /gene="rpsC"
                     /locus_tag="OQN13_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCS1615230.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5244..5654
                     /gene="rplP"
                     /locus_tag="OQN13_001858"
     CDS             5244..5654
                     /gene="rplP"
                     /locus_tag="OQN13_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCS1615231.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5654..5845
                     /gene="rpmC"
                     /locus_tag="OQN13_001859"
     CDS             5654..5845
                     /gene="rpmC"
                     /locus_tag="OQN13_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCS1615232.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5845..6099
                     /gene="rpsQ"
                     /locus_tag="OQN13_001860"
     CDS             5845..6099
                     /gene="rpsQ"
                     /locus_tag="OQN13_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCS1615233.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6264..6635
                     /gene="rplN"
                     /locus_tag="OQN13_001861"
     CDS             6264..6635
                     /gene="rplN"
                     /locus_tag="OQN13_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCS1615234.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6646..6960
                     /gene="rplX"
                     /locus_tag="OQN13_001862"
     CDS             6646..6960
                     /gene="rplX"
                     /locus_tag="OQN13_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCS1615235.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6975..7514
                     /gene="rplE"
                     /locus_tag="OQN13_001863"
     CDS             6975..7514
                     /gene="rplE"
                     /locus_tag="OQN13_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCS1615236.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7529..7834
                     /gene="rpsN"
                     /locus_tag="OQN13_001864"
     CDS             7529..7834
                     /gene="rpsN"
                     /locus_tag="OQN13_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCS1615237.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7868..8260
                     /gene="rpsH"
                     /locus_tag="OQN13_001865"
     CDS             7868..8260
                     /gene="rpsH"
                     /locus_tag="OQN13_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCS1615238.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8273..8806
                     /gene="rplF"
                     /locus_tag="OQN13_001866"
     CDS             8273..8806
                     /gene="rplF"
                     /locus_tag="OQN13_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCS1615239.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8816..9169
                     /gene="rplR"
                     /locus_tag="OQN13_001867"
     CDS             8816..9169
                     /gene="rplR"
                     /locus_tag="OQN13_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCS1615240.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9184..9687
                     /gene="rpsE"
                     /locus_tag="OQN13_001868"
     CDS             9184..9687
                     /gene="rpsE"
                     /locus_tag="OQN13_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCS1615241.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9691..9870
                     /gene="rpmD"
                     /locus_tag="OQN13_001869"
     CDS             9691..9870
                     /gene="rpmD"
                     /locus_tag="OQN13_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCS1615242.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9874..10308
                     /gene="rplO"
                     /locus_tag="OQN13_001870"
     CDS             9874..10308
                     /gene="rplO"
                     /locus_tag="OQN13_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCS1615243.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10316..11647
                     /gene="secY"
                     /locus_tag="OQN13_001871"
     CDS             10316..11647
                     /gene="secY"
                     /locus_tag="OQN13_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCS1615244.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11679..11795
                     /gene="rpmJ"
                     /locus_tag="OQN13_001872"
     CDS             11679..11795
                     /gene="rpmJ"
                     /locus_tag="OQN13_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCS1615245.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11942..12298
                     /gene="rpsM"
                     /locus_tag="OQN13_001873"
     CDS             11942..12298
                     /gene="rpsM"
                     /locus_tag="OQN13_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCS1615246.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12315..12704
                     /gene="rpsK"
                     /locus_tag="OQN13_001874"
     CDS             12315..12704
                     /gene="rpsK"
                     /locus_tag="OQN13_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCS1615247.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12738..13358
                     /gene="rpsD"
                     /locus_tag="OQN13_001875"
     CDS             12738..13358
                     /gene="rpsD"
                     /locus_tag="OQN13_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCS1615248.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13384..14373
                     /gene="rpoA"
                     /locus_tag="OQN13_001876"
     CDS             13384..14373
                     /gene="rpoA"
                     /locus_tag="OQN13_001876"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCS1615249.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14414..14797
                     /gene="rplQ"
                     /locus_tag="OQN13_001877"
     CDS             14414..14797
                     /gene="rplQ"
                     /locus_tag="OQN13_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCS1615250.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14904..15272
                     /locus_tag="OQN13_001878"
     CDS             14904..15272
                     /locus_tag="OQN13_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCS1615251.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15283..15708
                     /gene="zntR"
                     /locus_tag="OQN13_001879"
     CDS             15283..15708
                     /gene="zntR"
                     /locus_tag="OQN13_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCS1615252.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15764..15982
                     /gene="arfA"
                     /locus_tag="OQN13_001880"
     CDS             15764..15982
                     /gene="arfA"
                     /locus_tag="OQN13_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCS1615253.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15979..16389)
                     /gene="mscL"
                     /locus_tag="OQN13_001881"
     CDS             complement(15979..16389)
                     /gene="mscL"
                     /locus_tag="OQN13_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCS1615254.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16519..17895)
                     /gene="trkA"
                     /locus_tag="OQN13_001882"
     CDS             complement(16519..17895)
                     /gene="trkA"
                     /locus_tag="OQN13_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCS1615255.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17917..19206)
                     /gene="rsmB"
                     /locus_tag="OQN13_001883"
     CDS             complement(17917..19206)
                     /gene="rsmB"
                     /locus_tag="OQN13_001883"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCS1615256.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19252..20199)
                     /gene="fmt"
                     /locus_tag="OQN13_001884"
     CDS             complement(19252..20199)
                     /gene="fmt"
                     /locus_tag="OQN13_001884"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCS1615257.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20214..20723)
                     /gene="def"
                     /locus_tag="OQN13_001885"
     CDS             complement(20214..20723)
                     /gene="def"
                     /locus_tag="OQN13_001885"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCS1615258.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20853..21977
                     /gene="dprA"
                     /locus_tag="OQN13_001886"
     CDS             20853..21977
                     /gene="dprA"
                     /locus_tag="OQN13_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCS1615259.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21949..22422
                     /locus_tag="OQN13_001887"
     CDS             21949..22422
                     /locus_tag="OQN13_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCS1615260.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22451..22993
                     /locus_tag="OQN13_001888"
     CDS             22451..22993
                     /locus_tag="OQN13_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCS1615261.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22998..23570
                     /gene="tsaC"
                     /locus_tag="OQN13_001889"
     CDS             22998..23570
                     /gene="tsaC"
                     /locus_tag="OQN13_001889"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCS1615262.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23575..24393
                     /gene="aroE"
                     /locus_tag="OQN13_001890"
     CDS             23575..24393
                     /gene="aroE"
                     /locus_tag="OQN13_001890"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCS1615263.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            24390..24647
                     /locus_tag="OQN13_001891"
     CDS             24390..24647
                     /locus_tag="OQN13_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCS1615264.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24623..25177)
                     /locus_tag="OQN13_001892"
     CDS             complement(24623..25177)
                     /locus_tag="OQN13_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCS1615265.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6492 a         6022 c         6679 g         6300 t
ORIGIN      
        1 ttctttacaa acattggtag atcagaattt gccctggagc gcatcgctta aagtcgggga
       61 caaaaaattg cctgttgtgg tcgttcgcgt cggtgaggct agcgttgatg tcctcgtagg
      121 tcagcaaacg tggacgctga cacataaatg gtttgagtcg gtatggacgg gtgattatct
      181 tctgttgtgg aaaatgtcgc cggaagggga gagtaccatt acgcgtgata gcagcgagga
      241 agagattctc tggctggaaa cgatgttaaa ccgtgcattg catatttcga cagaaccttc
      301 ggcggagtgg cgtcccttgt tggtagaaaa aataaagcaa tttcaaaaaa gccaccactt
      361 gaaaactgac ggtgttgttg gcttttcaac gttagtacat ctctggcagg tggcagggga
      421 gagtgcctac ttatatcggg atgaagcaaa tatttccccg gaaacaacag tgaaggggaa
      481 atgacatgtt tgaattctat atcgccgcgc gtgagcagaa agaaacaggt caccctggta
      541 tcttctcccg acaaaaacac agcaccataa tatatgtcat ttgtttattg ctgatttgcc
      601 tgtggtttgc aggaatggtg ttggttggtg ggtatgccag gcagctatgg gcgctttgga
      661 tagtaaaagc agaagtcact gtagaggctg aaacgcccgc ttttaaacaa tcaacacaac
      721 actatttctt caaaaaacag ccactccccg tcgtcgaatc cgtcgaggag gaagatgacc
      781 cagacgtcgc ggttgagaat gctccctctt cttctgaaga cgaagaaaat actgtaaaag
      841 aaagcgacga gaaagccggt ttaagagagc gtgtcaaaaa tgcgctgaac gaacttgaga
      901 gataacccga aggctgttta ctttactagc aatacgcttg cgttcggtgg ttaagtatgt
      961 ataatgcgcg ggcttgtcgt agttgacagc aggttcaatc tgaacctcag ttagccgaat
     1021 ttggctacct aacaatgctc ccaatcgggg agctacgtaa gaacggttac actctcccat
     1081 caatcgtaat gggtctgagg agtaatcatt ttcgtttata aaataattgg agctctggtc
     1141 tcatgcagaa ccaaagaatc cgtatccgcc tgaaagcgtt tgatcatcgt ctgatcgatc
     1201 aagcaaccgc ggaaatcgtc gagactgcca agcgcactgg tgcgcaggtc cgtggtccga
     1261 tcccgctgcc gacacgcaaa gagcgcttca ctgttctgat ctccccgcac gtcaacaaag
     1321 acgcgcgcga tcagtacgaa atccgtactc acttgcgtct ggttgacatc gttgagccaa
     1381 ccgagaaaac cgttgatgct ctgatgcgtc tggatctggc tgccggtgta gacgtgcaga
     1441 tcagcctggg ttaatcaggt cattgagcga ttgagaggtt gaaacaatga ttggtttagt
     1501 cggtaaaaaa gtgggtatga cccgtatctt cacagaagac ggcgtttcta tcccagtaac
     1561 cgtaatcgaa gttgaagcaa accgcgttac tcaggttaaa gacctggcta acgatggcta
     1621 ccgtgctatt caggtgacca ccggtgctaa aaaagctaac cgtgtgacca agcctgaagc
     1681 tggccacttc gctaaagctg gcgtagaagc tggccgtggt ctgtgggaat tccgcctggc
     1741 tgaaggcgaa gagttcactg taggtcagag cattagcgtt gaactgtttg ctgaggttaa
     1801 aaaagttgac gtaactggca cctctaaagg taaaggtttc gcaggtaccg ttaagcgctg
     1861 gaacttccgt acccaggacg ctactcacgg taactccttg tctcaccgcg ttccgggttc
     1921 tatcggtcag aaccagactc cgggcaaagt gttcaaaggc aagaaaatgg caggtcagat
     1981 gggtaacgaa cgtgtaaccg ttcagagcct tgacgtagta cgcgttgacg ctgagcgcaa
     2041 cctgctgctg gttaaaggtg ctgtcccggg tgcaaccggt agcgacctga tcgttaaacc
     2101 agctgtgaag gcgtaaggag atagcaatgg aattagtatt gaaagacgcg cagagcgcgc
     2161 tgactgtttc cgaaactacc ttcggtcgtg atttcaacga agcgctggtt caccaggttg
     2221 ttgttgctta tgcagctggt gctcgtcagg gtactcgtgc tcagaagact cgtgctgaag
     2281 taactggttc cggtaaaaaa ccgtggcgcc agaaaggcac cggccgtgcg cgttctggtt
     2341 ctatcaagag cccgatctgg cgttctggtg gcgtgacctt tgctgctcgt ccgcaggacc
     2401 acagtcaaaa agttaacaag aagatgtacc gcggcgcgct gaaaagcatc ctgtccgaac
     2461 tggtacgtca ggatcgtctg atcgttgtcg agaagttctc tgtagaagcg ccgaaaacta
     2521 agctgctggc acagaaactg aaagacatgg ctctggaaga tgtgctgatc atcaccggtg
     2581 agctggacga aaacctgttc ctggctgcgc gcaacctgca caaggttgac gtacgcgatg
     2641 caactggtat cgacccggtt agcctgatcg ccttcgacaa agtcgtaatg actgctgatg
     2701 ctgttaagca agttgaggag atgctggcat gattcgtgaa gaacgtctgc tgaaggtgct
     2761 gcgtgcaccg cacgtttctg aaaaagcgtc tactgcgatg gaaaaatcca acaccatcgt
     2821 actcaaagtt gctaaagacg cgaccaaagc agaaatcaaa gctgctgtgc agaaactgtt
     2881 tgaagtcgaa gtcgaagtcg ttaacaccct ggtagttaaa gggaaagtta aacgtcacgg
     2941 acagcgtatc ggtcgtcgta gcgactggaa aaaagcttac gtcaccctga aagaaggcca
     3001 gaatctggac ttcgttggcg gcgctgagta agtcggagga gtaatacaat ggcagttgtt
     3061 aaatgtaaac cgacatctcc gggtcgtcgc cacgtagtta aagtggttaa ccctgagctg
     3121 cacaagggca aaccttttgc tccgttgctg gaaaaaaaca gcaaatccgg tggtcgtaac
     3181 aacaatggcc gtatcaccac tcgtcatatc ggtggtggcc acaagcaggc ttaccgtatt
     3241 gttgacttca aacgcaacaa agacggtatc ccggcagttg ttgaacgtct tgagtacgat
     3301 ccgaaccgtt ccgcgaacat cgcgctggtt ctgtacaaag acggtgaacg ccgttacatc
     3361 ctggccccta aaggcctgaa agctggcgac cagattcagt ctggcgttga tgctgcaatc
     3421 aaaccaggta acaccctgcc gatgcgcaac atcccggttg gttctactgt tcataacgta
     3481 gaaatgaaac caggtaaagg cggtcagctg gcacgttccg ctggtactta cgttcagatc
     3541 gttgctcgtg atggtgctta tgtcaccctg cgtctgcgtt ctggtgaaat gcgtaaagta
     3601 gaagcagact gccgtgcaac tctgggcgaa gttggcaatg ctgagcatat gctgcgcgtt
     3661 ctgggtaaag caggtgctgc acgctggcgt ggtgttcgtc cgaccgttcg cggtaccgcg
     3721 atgaacccgg tagaccaccc acatggtggt ggtgaaggtc gtaactttgg taagcacccg
     3781 gtaactccgt ggggcgttca gaccaaaggt aagaagaccc gcagcaacaa gcgtactgat
     3841 aaattcatcg tacgtcgccg tagcaaataa ttttagagga taagccatgc cacgttctct
     3901 caagaaaggt ccttttattg acctgcactt gctgaagaag gtagagaaag cggtggaaag
     3961 cggagacaag aagcccctgc gcacttggtc ccgtcgttca acgatctttc ctaacatgat
     4021 cggtttgacc atcgctgtcc ataatggtcg tcagcacgtt ccggtatttg tgaccgacga
     4081 aatggtcggt cacaaactgg gtgaattcgc accgactcgt acttatcgcg gccacgctgc
     4141 tgataaaaaa gcgaagaaga aataaggtag gaggaagaga tggaaactat cgctaaacat
     4201 cgccatgctc gttcttctgc tcagaaggtt cgccttgttg ctgacctgat tcgcggtaag
     4261 aaagtgtcgc aggctctgga tattttgacc tacaccaaca agaaagcggc tgtactggtc
     4321 aagaaagttc tggaatctgc cattgctaac gctgaacaca acgatggcgc tgacattgac
     4381 gatctgaaag ttacgaaaat tttcgtagac gaaggcccga gcatgaagcg cattatgccg
     4441 cgtgcaaaag gtcgtgcaga tcgcatcctg aagcgcacca gccacatcac tgtggttgtg
     4501 tccgatcgct gagactctgg agactagcaa tgggtcagaa agtacatcct aatggtattc
     4561 gcttgggtat tgtaaaacca tggaactcta cctggtttgc gaacaccaaa gaattcgctg
     4621 acaacctgga cagcgatttt aaagtacgtc agtacctgac taaggaactg gctaaagcgt
     4681 ctgtatctcg tatcgttatc gagcgtccgg ctaagagcat ccgtgtaacc attcacactg
     4741 ctcgcccggg tatcgttatc ggtaaaaaag gtgaagacgt agaaaaactg cgtaaggtcg
     4801 tagcggacat cgctggcgtt cctgcacaga tcaacatcgc cgaagttcgt aagcctgaac
     4861 tggacgcaaa actggttgct gacagcatca cttctcagct ggaacgtcgc gttatgttcc
     4921 gtcgtgctat gaagcgtgct gtacagaacg caatgcgtct gggcgctaaa ggtattaaag
     4981 ttgaagttag cggccgtctg ggcggcgcgg aaatcgcacg taccgaatgg taccgcgaag
     5041 gtcgcgtacc gctgcacact ctgcgtgctg acatcgacta caacacctct gaagcgcaca
     5101 ccacttacgg tgtaatcggc gttaaagtgt ggatcttcaa aggcgagatc ctgggtggta
     5161 tggctgctgt tgaacaaccg gaaaaaccgg ctgctcagcc taaaaagcag cagcgtaaag
     5221 gccgtaaata aggagcgtcg ctgatgttac aaccaaagcg tacaaaattc cgtaaaatgc
     5281 acaaaggccg taaccgcggt ctggcgcagg gtacggatgt tagcttcggc agcttcggtc
     5341 tgaaagctgt tggccgtggt cgtctgactg cccgtcagat cgaagcagca cgtcgtgcta
     5401 tgacccgtgc agttaagcgt caaggtaaga tctggatccg tgtgttcccg gacaaaccga
     5461 tcactgaaaa gccgctggca gtgcgtatgg gtaaaggtaa aggtaacgtg gagtattggg
     5521 ttgccttgat tcagccgggt aaagtcctgt atgaaatgga cggtgttccg gaagagctgg
     5581 cccgtgaagc attcaagctg gcagcagcga aactgccgat taaaaccacc tttgtaacta
     5641 agacggtgat gtaatgaaag caaaagagct gcgtgagaag agcgttgaag agctgaacac
     5701 cgagctgctg aacctgctgc gtgagcagtt caacctgcgt atgcaggctg caagtggcca
     5761 gctgcaacag tctcacctgt tgaagcaagt gcgtcgcgat gtcgcacgcg ttaagacttt
     5821 actgaacgag aaggcgggtg cgtaatgacc gataaaatcc gtactctgca aggtcgcgtt
     5881 gttagcgaca aaatggagaa atccattgtt gttgctatcg aacgttttgt gaaacacccg
     5941 atctacggta aattcatcaa gcgtacgacc aaactgcacg tacatgacga gaacaacgaa
     6001 tgcggtatcg gtgacgtggt tgaaatccgc gaatgccgtc cgctgtccaa gactaaatcc
     6061 tggacgctgg ttcgcgttgt agagaaagcg gttctgtaat acagtacact ctctcgatac
     6121 gaataaacgg ctcagaaatg agccgtttat tttttctacc catatccttg aagcggtgtt
     6181 ataatgccgc gccctcgata tggggatttt taacgacctg attttcgggt ctcagtagta
     6241 gttgacatta gcggagcact aaaatgatcc aagaacagac tatgctgaac gtcgccgaca
     6301 actccggtgc acgtcgcgta atgtgtatca aggttctggg tggctcgcac cgtcgctacg
     6361 caggcgtagg cgacatcatc aagatcacca tcaaagaagc aattccgcgt ggtaaggtca
     6421 aaaaaggtga tgtgctgaag gcggtagtgg tgcgcaccaa gaagggtgtt cgtcgcccgg
     6481 acggttctgt cattcgcttc gatggtaatg cttgtgttct tctgaacaac aacagcgagc
     6541 agcctatcgg tacgcgtatt tttgggccgg taactcgtga gcttcgtagt gagaagttca
     6601 tgaaaattat ctctctggca ccagaagtac tctaaggagc gaatcatggc agcgaaaatc
     6661 cgtcgtgatg acgaagttat cgtgttaacc ggtaaagata aaggtaaacg cggtaaagtt
     6721 aagaatgtcc tgtcttccgg caaggtcatt gttgaaggta tcaacctggt taagaaacat
     6781 cagaagccgg ttccggccct gaaccaaccg ggtggcatcg ttgaaaaaga agccgctatt
     6841 caggtttcca acgtagcaat cttcaatgcg gcaaccggca aggctgaccg tgtaggcttt
     6901 agattcgaag acggcaaaaa agtccgtttc ttcaagtcta acagcgaaac tatcaagtaa
     6961 tttggagtag tacgatggcg aaactgcatg attactacaa agacgaagta gttaaaaaac
     7021 tcatgactga gtttaactac aattctgtca tgcaagtccc tcgggtcgag aagatcaccc
     7081 tgaacatggg tgttggtgaa gcgatcgctg acaaaaaact gctggataac gcagcagcag
     7141 acctggcagc aatctccggt caaaaaccgc tgatcaccaa agcacgcaaa tctgttgcag
     7201 gcttcaaaat ccgtcagggc tatccgatcg gctgtaaagt aactctgcgt ggcgaacgca
     7261 tgtgggagtt ctttgagcgc ctgatcacta ttgctgtacc tcgtattcgt gacttccgtg
     7321 gcctgtccgc taagtctttc gacggtcgtg gtaactacag catgggtgtc cgtgagcaga
     7381 tcatcttccc agaaatcgac tacgataaag tcgaccgcgt tcgtggtttg gatattacca
     7441 ttaccactac tgcgaaatct gacgaagaag gccgcgctct gctggctgcc tttgacttcc
     7501 cgttccgcaa gtaaggtagg gttactaaat ggctaagcaa tcaatgaaag cacgcgaagt
     7561 aaaacgcgta gctttagctg ataaatactt cgcgaaacgc gctgaactga aagcgatcat
     7621 ctctgatgtg aacgcttccg acgaagatcg ttggaacgct gttctcaagc tgcagactct
     7681 gccgcgtgat tccagcccgt ctcgtcagcg taaccgctgc cgtcaaacag gtcgcccgca
     7741 tggtttcctg cggaagttcg ggttgagccg tattaaggtc cgtgaagccg ctatgcgcgg
     7801 tgaaatcccg ggtctgaaaa aggctagctg gtaattgtca ccaattgaat cacgggagta
     7861 aagacagatg agcatgcaag atccgatcgc ggatatgctg acccgtatcc gtaacggtca
     7921 ggccgcgaac aaagctgcgg tcaccatgcc ttcctccaag ctgaaagtgg caatcgccaa
     7981 cgtgctgaag gaagaaggtt ttattgaaga ttttaaagtt gaaggcgaca ccaagcctga
     8041 actggaactt actctgaagt atttccaggg caaagctgtt gtagaaagca ttcagcgtgt
     8101 cagccgccca ggtctgcgca tctataaacg taaagatgag ctgccgaaag ttatggcggg
     8161 tctgggtatc gcagttgttt ctacctctaa aggtgttatg actgatcgtg cagcgcgcca
     8221 ggctggtctt ggtggcgaaa ttatctgcta cgtagcctaa tcggaggaaa aaatgtctcg
     8281 tgttgctaaa gcaccggtcg ttgttcctgc cggcgttgac gtaaaaatca acggtcaggt
     8341 tattacgatc aaaggtaaaa acggcgagct gactcgtact ctcaacgatg ctgttgaagt
     8401 taaacatgca gataataccc tgaccttcgg tccgcgtgat ggttacgcag acggttgggc
     8461 acaggctggt accgcgcgtg ccctgctgaa ctcaatggtt atcggtgtta ccgaaggctt
     8521 cactaagaag ctgcagctgg ttggtgtagg ttaccgtgca gcggttaaag gcaatgtgat
     8581 taacctgtct ctgggtttct ctcatcctgt tgaccatcag ctgcctgcgg gtatcactgc
     8641 tgaatgtccg actcagactg aaatcgtgct gaaaggcgtt gataagcagg tgatcggcca
     8701 ggttgcagcg gatctgcgcg cctaccgtcg tcctgagcct tataaaggca agggtgttcg
     8761 ttacgccgac gaagtcgtgc gtaccaaaga ggctaagaag aagtaaggta acactatgga
     8821 taagaaattt gctcgtatcc gtcgtgcgac ccgcgcacgc cgcaagctcc aggagctggg
     8881 cgcaactcgc ctggtggtac atcgtacccc gcgtcacatt tacgcacagg taattgcacc
     8941 gaacggttct gaagttctgg tagctgcttc tactgtagaa aaagctatcg ctgaacaact
     9001 gaagtacacc ggtaacaaag acgcggctgc agctgtgggt aaagctgtcg ctgaacgcgc
     9061 tctggaaaaa ggcatcaaag atgtatcctt tgaccgttcc gggttccaat atcatggtcg
     9121 tgtccaggca ctggcagatg ctgcccgtga agctggcctt cagttctaag gtagaggtgt
     9181 aagatggctc acatcgaaaa acaagctggc gaactgcagg aaaagctgat cgcggtaaac
     9241 cgcgtatcta aaaccgttaa aggtggtcgt attttctcct tcacagctct gactgtagtt
     9301 ggcgatggta acggtcgcgt tggttttggt tacggtaaag cgcgtgaagt tccagcagcg
     9361 atccagaaag cgatggaaaa agcccgtcgc aatatgatta acgtcgcgct gaataacggc
     9421 actctgcaac accctgttaa aggtgttcac acgggttctc gcgtattcat gcagccggct
     9481 tccgaaggta ccggtatcat cgccggtggt gcaatgcgcg ccgttctgga agtcgctggg
     9541 gttcataacg ttctggctaa agcatatggt tccaccaacc cgatcaacgt ggttcgtgca
     9601 actattgatg gcctggaaaa tatgaattct ccagaaatgg tcgctgccaa gcgtggtaaa
     9661 tccgttgaag aaattctggg gaaataaacc atggcaaaga ctattaaaat tactcaaacc
     9721 cgcagtgcaa tcggtcgtct gccgaaacac aaggcaacgc tgcttggcct gggtctgcgt
     9781 cgtattggtc acaccgtaga gcgcgaggat actcctgcta ttcgcggtat gatcaacgcg
     9841 gtttccttca tggttaaagt tgaggagtaa gagatgcgtt taaatactct gtctccggcc
     9901 gaaggctcca aaaaggcggg taaacgcctg ggtcgtggta tcggttctgg cctcggtaaa
     9961 accggtggtc gtggtcacaa aggtcagaag tctcgttctg gcggtggcgt acgtcgcggt
    10021 ttcgagggtg gtcagatgcc tctgtaccgt cgtctgccga aattcggctt cacttctcgt
    10081 aaagcagcga ttacagccga agttcgtctg tctgacctgg ctaaagtaga aggcggtgtt
    10141 gtagacctga acacgctgaa agcggctaac attatcggta tccagatcga gttcgcgaaa
    10201 gtgatcctgg ctggcgaagt aacgactccg gtaactgttc gtggcctgcg tgttactaaa
    10261 ggcgctcgtg ctgctatcga agctgctggc ggtaaaatcg aggaataagt agcagatggc
    10321 taaacaaccg ggattagatt ttcaaagtgc caaaggtggc ttaggcgagc tgaaacgcag
    10381 actgctgttt gttatcggtg cgctgattgt gttccgtatt ggctctttta ttccgatccc
    10441 tggtattgat gccgctgtac ttgccaaact gcttgagcaa cagcgaggca ccatcattga
    10501 gatgtttaac atgttctctg gtggtgctct cagccgtgct tctatctttg ctctggggat
    10561 catgccgtat atttcggcgt cgatcattat ccagctgctg acggtggttc acccaacgtt
    10621 ggcggaaatt aagaaagaag gggagtctgg tcgtcgtaag atcagccagt acacccgcta
    10681 cggtactctg gtgctggcaa tattccagtc gatcggtatt gctactggtc tgccgaatat
    10741 gcctggtatg caaggcctgg tgattaaccc gggctttgca ttctacttca ccgctgttgt
    10801 aagtctggtc acaggaacga tgttcctgat gtggttgggc gaacagatta ctgaacgagg
    10861 tatcggcaac ggtatttcaa tcattatctt cgtcggtatt gtcgcgggac tcccgccagc
    10921 cattgcccat actatcgagc aagcgcgtca aggcgacctg cacttcctcg tgttgctgtt
    10981 ggttgcagta ttagtatttg cagtgacgtt ctttgttgta tttgttgagc gtggtcaacg
    11041 ccgcattgtg gtaaactacg cgaaacgtca gcaaggtcgt cgtgtttatg ctgcacagag
    11101 cacacattta ccgctgaaag tgaatatggc gggggtaatc ccggcaatct tcgcttccag
    11161 tattattctg ttcccggcga ccatcgcgtc atggttcggg ggcggtactg gttggaactg
    11221 gctgacaaca atttcgctgt atttgcagcc tgggcaaccg ctttatgtgt tactctatgc
    11281 gtctgcaatc atcttcttct gtttcttcta cacggcgttg gttttcaacc cgcgtgaaac
    11341 agcagataac ctgaagaagt ccggtgcatt tgtaccagga attcgtccgg gagagcaaac
    11401 ggcgaagtat atcgataaag taatgacccg cctgaccctg gttggtgcgc tgtatattac
    11461 ctttatctgc ctgatcccgg agttcatgcg tgatgcaatg aaagtaccgt tctacttcgg
    11521 tgggacctca ctgcttatcg ttgttgtcgt gattatggac tttatggctc aagtgcaaac
    11581 tctgatgatg tccagtcagt atgagtctgc attgaagaag gcgaacctga aaggctacgg
    11641 ccgataattg gtcgcccgag aagttacgga gagtaaaaat gaaagttcgt gcttccgtca
    11701 agaaattatg ccgtaactgc aaaatcgtta agcgtgatgg tgtcatccgt gtgatttgca
    11761 gtgccgagcc gaagcataaa cagcgccaag gctgattttt tcgcatattt ttcttgcaaa
    11821 gttgggttga gctggctaga ttagtcagcc aatcttttgt atgtctgtgc gtttccattt
    11881 gagtatcctg aaaacgggct tttcagcatg gaacgtacat attaaatagt aggagtgcat
    11941 agtggcccgt atagcaggca ttaacattcc tgatcataag catgccgtaa tcgcattaac
    12001 ttcgatttat ggcgtcggca agacccgttc taaagccatc ctggctgcag cgggtatcgc
    12061 tgaagatgtt aagatcagtg agctgtctga aggacaaatc gacacgctgc gtgacgaagt
    12121 tgccaaattt gtcgttgaag gtgatctgcg ccgtgaaatc agcatgagca tcaagcgcct
    12181 gatggatctt ggttgctatc gcggtttgcg tcatcgtcgt ggtctcccgg ttcgcggtca
    12241 gcgtaccaag accaacgcac gtacccgtaa gggtccgcgc aaaccgatca agaaataatc
    12301 ggggtgattg aataatggca aaggcaccaa ttcgtgcacg taaacgtgta agaaaacaag
    12361 tctctgacgg cgtggctcat atccatgctt ctttcaacaa caccatcgtg actatcactg
    12421 atcgtcaggg taacgcgttg ggttgggcaa cagccggtgg ttccggtttc cgtggttctc
    12481 gcaaatccac tccgtttgca gctcaggttg cagcagagcg ttgcgctgac gccgtgaaag
    12541 aatacggcat caagaatctg gaagttatgg ttaaaggtcc gggtccaggc cgcgaatcta
    12601 ctattcgtgc tctgaacgcc gcaggtttcc gcatcactaa cattactgat gtgactccga
    12661 tccctcataa cggttgtcgt ccgccgaaaa aacgtcgcgt ataacgcctc gttttccagg
    12721 tttgttggag aaagaaaatg gcaagatatt tgggtcctaa gctcaagctg agccgtcgtg
    12781 agggcaccga cttattcctt aagtctggcg ttcgcgcgat cgataccaag tgtaaaattg
    12841 aacaagctcc tggccagcac ggtgcgcgta aaccgcgtct gtctgactat ggtgtgcagt
    12901 tgcgtgaaaa gcaaaaagtt cgccgtatct atggtgtgct ggagcgtcag ttccgtaact
    12961 actacaaaga agcagcacgt ctgaaaggca acaccggtga aaacctgttg gctctgctgg
    13021 aaggtcgtct ggacaacgtt gtataccgta tgggcttcgg tgccactcgt gcagaagcac
    13081 gtcagctggt tagccataaa gcaattatgg taaacggtcg tgttgttaac atcgcttctt
    13141 atcaggttag tccgaatgac gttgtaagca ttcgtgagaa agcgaagaag cagtctcgcg
    13201 tgaaagccgc tctggagctg gctgagcagc gtgaaaagcc aacctggctg gaagttgatg
    13261 ctggcaagat ggaaggtacg tttaagcgta agccggagcg ttctgatctg tctgcggaca
    13321 ttaacgaaca cctgatcgtc gagctttact ccaagtaaag cttagtacca aagagaggac
    13381 acaatgcagg gttctgtgac agagtttcta aaaccgcgcc tggttgatat cgagcaagtg
    13441 agttcgacgc acgccaaggt gacccttgag cctttagagc gtggctttgg ccatactctg
    13501 ggtaacgcac tgcgccgtat tctgctctca tcgatgccgg gttgcgcggt gaccgaggtt
    13561 gagattgatg gtgtactaca tgagtacagc accaaagaag gcgttcagga agatatcctg
    13621 gaaatcctgc tcaacctgaa agggctggcg gtgagagttc agggcaaaga tgaagttatt
    13681 cttaccttga ataaatctgg cattggccct gtgactgcag ccgatatcac ccacgacggt
    13741 gatgtcgaaa tcgtcaagcc gcagcacgtg atctgccacc tgaccgatga gaacgcgtct
    13801 attagcatgc gtatcaaagt tcagcgcggt cgtggttatg tgccggcttc tacccgaatt
    13861 cattcggaag aagatgagcg cccaatcggc cgtctgctgg tcgacgcatg ctacagccct
    13921 gtggagcgta ttgcctacaa tgttgaagca gcgcgtgtag aacagcgtac cgacctggac
    13981 aagctggtca tcgaaatgga aaccaacggc acaatcgatc ctgaagaggc gattcgtcgt
    14041 gcggcaacca ttctggctga acaactggaa gctttcgttg acttacgtga tgtacgtcag
    14101 cctgaagtga aagaagagaa accagagttc gatccgatcc tgctgcgccc tgttgacgat
    14161 ctggaattga ctgtccgctc tgctaactgc cttaaagcag aagctatcca ctatatcggt
    14221 gatctggtac agcgtaccga ggttgagctc cttaaaacgc ctaaccttgg taaaaaatct
    14281 cttactgaga ttaaagacgt gctggcttcc cgtggactgt ctctgggcat gcgcctggaa
    14341 aactggccac cggcaagcat cgctgacgag taaccggatc acaggttaag gttttactga
    14401 gaaggataag gtcatgcgcc atcgtaagag tggtcgtcaa ctgaaccgca acagcagcca
    14461 tcgccaggct atgttccgca atatggcagg ttcactggtt cgtcatgaaa tcatcaagac
    14521 gactctgcct aaagcgaaag agctgcgccg cgtagttgag ccgctgatta ctcttgccaa
    14581 gactgatagc gttgctaatc gtcgtctggc atttgcccgt actcgtgata acgagatcgt
    14641 ggcaaaactg tttaacgaac tgggcccgcg tttcgcgagc cgtgccggtg gttacactcg
    14701 tattctgaag tgtggcttcc gtgcaggcga caacgcgccg atggcttaca tcgagctggt
    14761 tgatcgttca gagaaagcag aagctgctgc agagtaatct gaagcaatgt aaaaaaaccc
    14821 gccccggcgg gtttttttat acccgtagta tccccactta tctacaatag ctgtactctt
    14881 tttgttcatc ccctggagta tttatgtggt tacttgacca gtgggcagag cgccatatag
    14941 cagaagcgca agcgaaaggt gagtttgata acctggcagg tagcggcgaa ccattgatac
    15001 tggatgatga ttctcacgtg ccaccggaat tacgtgcggg gtatcgcttg ctgaagaatg
    15061 ccggttgctt accgccagaa cttgagcaac ggagagaagc aattcagctt ctggatattc
    15121 tcaaaggtat ccgtcacgat gatccgcaat atcaagaggt tagccgtcga ttgtcattac
    15181 tggaattgaa gctgcgacaa gctggattga gtaccgattt tttacgcggc gattatgctg
    15241 acaagttgtt ggacaaaatc aacgataact agtggagtat gtatgtatcg cattggtgag
    15301 ctggcaaaaa tggcggaagt aacacctgac acgattcgtt attacgaaaa acagcagatg
    15361 atggagcatg aagtgcgtac tgaaggcggg tttcgcctgt ataccgaaag cgatctccag
    15421 cgattgaaat ttatccgcca tgccagacaa ctaggtttca gtctggagtc gatccgcgag
    15481 ttgctgtcga tccgcatcga tcctgaacac catacctgtc aggagtcaaa aggcattgtg
    15541 caggaaagat tgcaggaagt cgaagcacgg atagccgagt tgcagagtat gcagcgttcc
    15601 ttgcaacgcc ttaacgatgc ctgttgtggg accgcccata gcagtgttta ttgctcgatt
    15661 cttgaagctc ttgaacaagg ggcgagtggc gttaagagtg gctgttgatt ttttgcactg
    15721 acaggattac actcgcgccg ttaaataacc aactggagtt tttatgagcc gatatcagca
    15781 tactaaaggg cagataaagg ataatgcgat agaagcatta ctacatgatc ccttattccg
    15841 acagcgagta gagaaaaata agaaagggaa aggcagttac atgcgaaaag gaaaacatgg
    15901 caatcggggg aactgggagg ccagtggcaa gaaagtaaat cactttttta ccactggtct
    15961 tctgctttca ggcgcttgtt aagagcggtt attctgctct ttcagcaaat cacgaatttc
    16021 tgccagtaat acttcttctt tagttggtgc aggtgcggca gctggttctt cttttttccg
    16081 attcagtttg ttgattagct taatcgccat aaagatggca aaggccacaa tcagaaaatc
    16141 aaagacgttt tgaatgaaga caccgtaatg catcacaaca gcagggatat ccccctgcgc
    16201 atcgcgtagc gtgacagcaa actgtttaaa gtcgatcccg ccaattaata agcccagagg
    16261 tggcatgatg atatcggcaa ccagtgaaga tacaatcttc ccgaatgccg caccgataat
    16321 gacacccacc gccaaatcca ccacgttccc gcgcatcgca aattcgcgaa attctttaat
    16381 aatgctcatg ttattctccc tatgaagccg acaaccataa gtctaacaaa tgttaagcca
    16441 ttttcctgcc aggaatgaat ttaattaatc cttgataatc aaagggaaat aataaggcgt
    16501 cattagacgc cttattaatt acaagaagaa agggcttggc tggaagagtc tttcgacgtc
    16561 ggtaataaac tttttatctg tgaggaacat aattacgtga tcgccttgct caatgcgcag
    16621 attgtcattg gcaatcatca cgtcgtttcc acgtaccacc gctccaataa tcgttcctgg
    16681 cggtagcttg atttcgtcaa tgactctgcc gacaacgcgt gaggtgcttt catcaccgtg
    16741 agcaacggct tcaatagctt ctgctacgcc gcggcgcaat gaggaaacac caacaatatc
    16801 tgcttttcgc acatggctaa gcaacgcaga aatagttgct tgttgtggtg aaattgcaat
    16861 atcgatcacg ctcccctgaa ccagatccac ataagcgcga cgctggatca ataccatcac
    16921 ctttttcgca cccatacgtt tggcaagcat ggcggacatg atattggcct cgtcatcgtt
    16981 ggtgacggca ataaacagat caacttgatc gatatgttct tcggccagca gttcttgatc
    17041 cgacgcatca ccaaaaaaga cgatcgtatt ctgcaacttt tccgccagtt cggcagcgcg
    17101 ctgctgatta cgttcgatga gtttaacgct gtaatctttt tccagacgac gcgccagccc
    17161 tgcaccgata ttaccgccac caaccagcat gatccgctta tacggttttt ccagtcgctg
    17221 taattcactc atcaccgcgc ggatatgctg tgaagcggca ataaagaaca cttcatcacc
    17281 agcttcaaca atggtcgaac cttgtggacg aatggggcga tcgtggcgga aaattgctgc
    17341 cacacgagta tcgatatgtg gcatatgttc gcgcatggtc gaaagtgcat taccaatcag
    17401 cgggccgcca taataggctt taactacagc gaggctgact ttaccctcag cgaagttcac
    17461 cacctgcaat gcgcctggat actcaatcag tcggtaaata ttatcgataa ccaactgctc
    17521 tggtgcgatc agatgatcaa tcggtacagc atctgaatga aatagcttat cggcatcgcg
    17581 tacgtagtct ggtgagcgga tgcgagcgat gcgattaggg gtgttgaaaa gtgagtaggc
    17641 tacctggcag gcaaccatat tggtttcatc tgaactggtt acagcaacca gcatatcggc
    17701 gtcgtcggca cctgcctccc gcaatacgcg tggatgagag ccatgcccct gcacgactcg
    17761 caggtcaaat ttatcctgca aggtccgcag acgctcaccg ttggtatcga caacagtaat
    17821 atcgttgttc tcgccaacca ggttttccgc cagtgtgccg ccaacctggc cggcacccag
    17881 aatgataatt ttcatcagtc tcgacccgtt atctcatcac tttttgatta gcttagcgta
    17941 aaagaagccg tcgccctctt cggcaccagg cagattttgt ttacccggtt gctctggtgt
    18001 tcctgtttcg caaagttcgg catcagcggt acgttgcaaa aaagctttaa tctgcaggct
    18061 attctcttcc ggtaacaccg aacaggtggc atagaccagg gttccaccgg attttaaatg
    18121 cggccaaatg gcgtcgagaa tttcagactg caattgcgcg agttccggga tatcgcgatc
    18181 gcgacgtaac catttaatat ccggatggcg acgaatcacc cccgttgctg aacaaggcgc
    18241 atctaataaa atgcgatcaa actgttgctc gccacaccat tgggaagggt aacggccatc
    18301 accttgtttc acggtcgcct tcataccaag gcgttttaaa ttgtcgtgaa cgcgagagag
    18361 gcgctgttcg tcaatatcaa ccgcaacaac ctgcgcttct ggtgccacct caaggatatg
    18421 cgttgtttta ccgccggggg cggcacaaag atccagaatg tgttcaccgt tttgtggcgc
    18481 aagccaggtc atgcaacctt gtgctgatgc atcctgaacg gtaacccatc cgtcttcaaa
    18541 accaggtagc gcatgaacag gtgcaggtgt ttccagccgt acagcatcag ggtaatccgc
    18601 atgcgggaaa cctttcattc ctgcttcatc caacaatgca agccagctgt cgcgggaatg
    18661 atgcgtacga ttaacgcgca gccacattgg cggacgctgg ttattggctt cgacgatgga
    18721 ttgccactgc tctggatacg ctttttgcag acgcttcagc aaccaggaag gatgcagata
    18781 acgtgcatca ctggtattaa actcggctaa taactcttct tgctgacgct ggaactggcg
    18841 taatacaccg ttaatcaacc ctttaagttg cggacgctta attgcgatag cgccttcaac
    18901 cgtttcagcc agcgcagcat gaggtggaat gcgggtataa agcagttgat acaaaccaac
    18961 cataatcagg taatgcacag tgcgctgttt gccggtcatc ggacgggcca ttaacttatt
    19021 aatcagccag tctaactgcg aaagcgtacg cagtacgcca aagcacaact cttgaagaag
    19081 tgctttgtct ttatcagaaa ctttttgctg gagcggtggc agaatgttgc ttaatgattg
    19141 cccttgctcg acgacttgtt caacggcctg ggccgccatg ctacgtaaat tacgttgttt
    19201 tttcataagt ataaaaatgc ccggtaagac cgggcttaga agagtggact atcaggccag
    19261 acggttgccc ggaacaaacc attcccgacg agaattcagg aggtcttgcg cgctcatcgc
    19321 tttcttaccc gcaggttgta acgagagcag gttcaggatg ccatcaccag tcgcaacctg
    19381 aatgccttgt ttgttggctt caaggatcgt tcctggtgca gcgttggttg ccgtatcaat
    19441 gaccgacgct ttccagactt taaccggttg tccttcaatt tccagccagc tcattggcca
    19501 tgggttgaaa gcgcgaatgc agcgttcaag ctgcgctgcc gaaagcgacc agtcaatacg
    19561 cgcttcttct ttactcaact tctcggcgta agtgacaaga gtttcgtcct gaacttctgg
    19621 tttcgccgtg ccgtctgcta gttgtttcaa cgtggtgatt agcccttgtg ggccaagctc
    19681 tgccagcttg tcgtacagcg taccactggt atcttctgca gtaatcgggc aggagagctt
    19741 atagagcata tcaccggtgt ctaaaccgac atccatttgc ataatggtca caccagtttc
    19801 tgcatcaccc gcccatagtg agcgttggat tggtgcagca ccgcgccagc gtggcagcag
    19861 tgaaccatga acgttgatac agccaagacg cggcatctcc agcactgctt taggcagaat
    19921 taagccatag gcgacgacga ccataacatc agcctgcaga tcggcgacca gttgctggct
    19981 ttcttgtgga cgcagggaaa caggttgaaa aacgggcaga cctttttcct cagccagaac
    20041 tttaaccggg ctgggcatca gttttttacc gcgtcctgcc ggtcggtctg gctgggtgaa
    20101 cacgccaacg acgttatgac cagaagacaa cagcgcgtcg agatgacgcg ctgcaaagtc
    20161 aggtgtaccc gcaaaaataa tacgtagtga ttctgacacg ttagttctta tccttaagcc
    20221 cgggctttca gacgatctag tttttcaact ttctgacgaa tacgttgttg tttcagcggt
    20281 gacagataat ccataaacag tttgccgacc aggtgatcca tctcatgctg aatacagatg
    20341 gctaacagcc cctctgcttc cagttcaaat ggtttaccgt cgcggtcaag ggcgcgaatt
    20401 ttaactttct ctgcgcgcgg cactaaagca cgttgttcag ggatcgacag gcaaccttct
    20461 tcaatgcctg tttcgccgct tttttctaaa agctccggat tgattaacac cagccgttcg
    20521 tcacggtttt ccgaaacatc aataacaatg atacgttgat ggatatcaac ctgggttgcc
    20581 gccaggccaa taccttcttc tgcgtacatc gtctcgaaca tatcatcgac gatacgctgt
    20641 atttctgcat tcacttcttc taccggttta gcaactttgc gaagccgctc gtccggaata
    20701 tgtaacactt gcaaaactga cataaatctc cagagatgtg ttcaggggtt agaaagatta
    20761 tttcttctat tctagacaaa tccccctctg attgacagca tcactgacca atcgcaaaga
    20821 ttgctaaggc tgcttatggc agggagataa ggatggtcga tacagaaatt tggctgcgtt
    20881 taatgagtat cagcagcttg tacggcgatg atatggtccg tatagctcac tggctggcaa
    20941 aacagtcgca aattgatgcg gttggattgc agcaaacagg gcttacattg cggcaggcac
    21001 aacgctttct ttcatttccg cgaaagagta tcgaaagctc actttgttgg ttggagcaac
    21061 ccaaccatca tttaatccct gcggacagcg aattttatcc tcctcaactt ctggtgacga
    21121 cagattaccc cggcgcactg tttgttgaag gagaactgca cgcgctgcat tcatttcagc
    21181 ttgccgtagt ggggagtcgg gcgcattcat ggtatggcga gcgatgggga cggttatttt
    21241 gcgaaactct ggcgacgcgt ggagtgacaa ttacgagtgg actggcgcgt ggaatcgatg
    21301 gtgtggcgca taaagctgcc ttacaggtaa atggcgtcag cattgctgta ttggggaatg
    21361 gacttaatac cattcatccc cgccgccatg cccgactggc tgccagtctg cttgaacatg
    21421 gcggagctct cgtctcggaa tttcccctcg atgttccacc ccttgcttac aatttcccac
    21481 gaagaaatcg cattatcagt ggtctaagta aaggtgtact ggtggtggaa gcggctttgc
    21541 gcagtggttc gctggtgaca gcacgttgtg cgcttgagca gaggcgtgaa gtttttgcct
    21601 tgccaggtcc aatagggaat ccgggaagcg aagggcctca ctggttaata aaacaaggtg
    21661 cgattcttgt gacggaaccg gaagaaattc tggaaaactt gcaatttgga ttgcactggt
    21721 tgccagacgc ccctgaaaat tcattttatt caccagatca gcaagacgtg gcattgccat
    21781 ttcctgagct cctggctaac gtaggagatg aggtaacacc tgttgacgtc gtcgctgaac
    21841 gtgccggcca acctgtgcca gaggtagtta ctcaactact cgaactggag ttagcaggat
    21901 ggatcgcagc tgtacccggc ggctatgtcc gattgaggag ggcatgccat gttcgacgta
    21961 ctaatgtatt tgtttgaaac ctatattcac actgaagctg agttgcgtgt ggatcaagac
    22021 aaacttgaac aggatcttac cgacgcaggt tttgagcgag aagatatcta caatgccctg
    22081 ctatggctgg aaaaacttgc tgattatcag gaagggttgg cagaaccgat gcaactggcc
    22141 tcggatcctc tctccatgcg tatttataca ccggaagagt gtgaacgact ggatgccagc
    22201 tgccgtgggt ttctgctttt ccttgagcag attcaggtgc tcaaccttga aactcgtgaa
    22261 atggtgatag agcgagtgct cgcgctggat aacgcagagt tcgaactgga tgatctgaaa
    22321 tgggtgatcc tgatggtgtt gttcaacatc ccggggtgcg aaaatgcgta ccagcaaatg
    22381 gaagaattac tctttgaagt gaatgaaggt atgctgcatt aatcatttct ttaattcagc
    22441 ataagttgtt atggcaaaat cagcactgtt cacggtgcgt aataatgagt cctgcccaaa
    22501 gtgcggggct gaactggtta ttcgatccgg gaaacacggt ccgtttcttg gatgctcaca
    22561 gtatccggcg tgtgactacg tccgtcttct gaaatcttca gcggatggac atattgtcaa
    22621 agttctggag gggcaggttt gccctgcatg tggcgcaaat ctggtattac gccagggacg
    22681 ctttggtatg tttattggtt gcagtaacta ccctgaatgc gaacataccg aacttatcga
    22741 taaaccggac gaaacagcaa ttacatgccc ccaatgtcgg acgggccatc tggtccagcg
    22801 ccgctcccgt tatggcaaaa catttcactc ctgtgatcgc tacccggagt gtcaatttgc
    22861 cattaacttc aaaccaatag ctggagaatg ccctgagtgt cattatccgc tactcatcga
    22921 aaagaaaacc gcgcagggtg taaaacactt ttgtgccagt aaacaatgtg gaaagccggt
    22981 ttcggcggaa taataacgtg aataataacc tgcaaggaga cgctatcgca gctgcgatag
    23041 atgtcctcaa tgaagaacgt gtcatcgcct atccaacgga agccgttttc ggtgttgggt
    23101 gcgatcctga tagcgaaaca gcagtgatga gactgttgga gttaaaacag cgtccggttg
    23161 ataaggggct gattttaatc gcggcaaatt acgagcagct taaaccctat attgatgaca
    23221 ccatgctgac tgatgcgcag cgtgaaacca ttttttcccg ctggccaggt cctgtcacct
    23281 ttgtctttcc cgcgcctgcg acaacaccgc gctggttgac gggccgcttt gattcgcttg
    23341 ctgtacgagt caccgaccat ccgttggtgg ttgctttgtg ccaggcttat ggtaaaccgc
    23401 tggtttctac cagtgccaac ttgagtggat tgccaccttg tcgaacagta gacgaagttc
    23461 gcgcacaatt tggcgcggcg ttcccggttg tgcctggtga aacggggggg cgtttaaatc
    23521 cttcagaaat ccgcgatgcc ctgacgggtg aactgtttcg acaggggtaa cataatggaa
    23581 acctatgctg tttttggtaa tccgatagcc cacagcaaat cgccattcat tcatcagcaa
    23641 tttgctcagc aactgaatat tgaacatccc tatgggcgcg tgttggcacc catcaatgat
    23701 ttcatcaaca cactgaacgc tttctttcgt gctggtggta aaggtgcgaa tgtgacggtg
    23761 ccttttaaag aagaggcttt tgccagagcg gatgagctta ctgaacgggc agcgttggct
    23821 ggtgcagtta ataccctcaa gcggttagaa gatggacgcc tgctgggtga caataccgat
    23881 ggtgtaggct tgttaagcga tctggaacgt ctgtctttta tccgccctgg tttacgtatt
    23941 ctgcttatcg gcgctggtgg agcatctcgc ggcgtactac tgccacttct ttccctagac
    24001 tgtgcggtga caatcactaa tcggacggta tcccgcgcgg aagagttggc taaattgttt
    24061 gcgcacactg gcagtattca ggcgttgggt atggacgaac tggaaggtca tgagtttgat
    24121 ctcattatta atgcaacatc cagtggcatc agtggtgata ttccggcgat cccgtcatcg
    24181 ctcattcatc caggcattta ttgctatgac atgttctatc agaaaggaaa aactcctttt
    24241 ctggcatggt gtgagcagcg aggctcaaag cgtaatgctg atggtttagg aatgctggtg
    24301 gcacaggcgg ctcatgcctt ccttctttgg catggcgttc tgcctgatgt agaaccggtc
    24361 atcaaactat tgcaacagga attgtcagcg tgaatcaggc catccagttt ccggataggg
    24421 aagagtggga cgagaataaa agatgtgtat gttttcctgc tctcgtgaat ggtatgcaac
    24481 tgacatgtgc gatctctggc gagagtctgg cgtatcgctt tactggaggt acgccagaac
    24541 agtggttagc gagttttcgt cagcatcgct gggacctgga agaagaagcg gaaaacttaa
    24601 ttcaggaaca aagtgaagat gatcaaggtt gggtctggtt accttgatcc agatattcgt
    24661 ccttccattt cacgtagtta tttgcggaat agcgtaaccc agccttctct tcatcactta
    24721 acgggcggat ctgtttgacg ggactaccga gatacagata tccgctctcc agccgtttat
    24781 tttgtgggac caggctaccc gcaccaatca tcacatcatc ttctactatt gcgccatcaa
    24841 gtaaaattga gcccatccca accaaaactc gattgccaat ggtgcagccg tggagcatca
    24901 ccttgtgacc aacagtgaca tcttcgccaa tggttaatgg gttgccatct gggttgtacg
    24961 aggatttatg agtgacatgc aacatactgc catcctggat attggtgcgt gctccgatct
    25021 gtacataatg tacatctcca cgaatcacaa cgagcggcca gatccccaca tcatcagcca
    25081 gacgaacgtc accaatcacg acgctgctat cgtcgatcat tacgcgctgg ccgatttgtg
    25141 gaaaaagatc gcggtatggg cgtaaaacat cagacatact cacctcaaca ataaatgatt
    25201 tcctaatgac tttgggtgca ttattagcct tgtgcaagtc ttttagtatg caaaaaagca
    25261 ccgttttgtg tgcgaatgca gcaaaaaggg tgaaaaaaca acaaacagaa aaaaagatca
    25321 aaaaagtact tgtgcaaaaa attgggatcc ctataatgcg cctccgttga gacgacaacg
    25381 tgaaacactt cacaggatgg tcggaacaac gaagagaaaa aattctgaaa ttcagggttg
    25441 actctgaaag aggaaagcgt aatatacgcc acctcgcgac agtgagctga aag
//
LOCUS       DAJVRF010000051        25430 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJVRF010000051 DAJVRF010000000
VERSION     DAJVRF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.051"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="OQN13_001893"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="OQN13_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCS1615266.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="OQN13_001894"
     CDS             complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="OQN13_001894"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCS1615267.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2464..2744
                     /locus_tag="OQN13_001895"
                     /pseudo
     CDS             2464..2744
                     /locus_tag="OQN13_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="OQN13_001896"
     CDS             complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="OQN13_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCS1615268.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3633..6455
                     /gene="uvrA"
                     /locus_tag="OQN13_001897"
     CDS             3633..6455
                     /gene="uvrA"
                     /locus_tag="OQN13_001897"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCS1615269.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6490..6846)
                     /locus_tag="OQN13_001898"
     CDS             complement(6490..6846)
                     /locus_tag="OQN13_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS1615270.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6850..7266)
                     /locus_tag="OQN13_001899"
     CDS             complement(6850..7266)
                     /locus_tag="OQN13_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCS1615271.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="OQN13_001900"
     CDS             complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="OQN13_001900"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCS1615272.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            8492..8714
                     /locus_tag="OQN13_001901"
                     /pseudo
     CDS             8492..8714
                     /locus_tag="OQN13_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9218..10411)
                     /gene="tyrB"
                     /locus_tag="OQN13_001902"
     CDS             complement(9218..10411)
                     /gene="tyrB"
                     /locus_tag="OQN13_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCS1615273.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(10664..11743)
                     /gene="alr"
                     /locus_tag="OQN13_001903"
     CDS             complement(10664..11743)
                     /gene="alr"
                     /locus_tag="OQN13_001903"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCS1615274.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(11796..13211)
                     /gene="dnaB"
                     /locus_tag="OQN13_001904"
     CDS             complement(11796..13211)
                     /gene="dnaB"
                     /locus_tag="OQN13_001904"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCS1615275.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13294..14277
                     /locus_tag="OQN13_001905"
     CDS             13294..14277
                     /locus_tag="OQN13_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCS1615276.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(14443..14685)
                     /gene="pspG"
                     /locus_tag="OQN13_001906"
     CDS             complement(14443..14685)
                     /gene="pspG"
                     /locus_tag="OQN13_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCS1615277.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(14819..15856)
                     /gene="dusA"
                     /locus_tag="OQN13_001907"
     CDS             complement(14819..15856)
                     /gene="dusA"
                     /locus_tag="OQN13_001907"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCS1615278.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(16219..17204)
                     /locus_tag="OQN13_001908"
                     /pseudo
     CDS             complement(16219..17204)
                     /locus_tag="OQN13_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            17522..18037
                     /gene="zur"
                     /locus_tag="OQN13_001909"
     CDS             17522..18037
                     /gene="zur"
                     /locus_tag="OQN13_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCS1615279.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(18079..18288)
                     /locus_tag="OQN13_001910"
     CDS             complement(18079..18288)
                     /locus_tag="OQN13_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCS1615280.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(18404..19729)
                     /gene="dinF"
                     /locus_tag="OQN13_001911"
     CDS             complement(18404..19729)
                     /gene="dinF"
                     /locus_tag="OQN13_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCS1615281.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(19802..20410)
                     /gene="lexA"
                     /locus_tag="OQN13_001912"
     CDS             complement(19802..20410)
                     /gene="lexA"
                     /locus_tag="OQN13_001912"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCS1615282.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(20520..20888)
                     /gene="dgkA"
                     /locus_tag="OQN13_001913"
     CDS             complement(20520..20888)
                     /gene="dgkA"
                     /locus_tag="OQN13_001913"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCS1615283.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            21059..23482
                     /gene="plsB"
                     /locus_tag="OQN13_001914"
     CDS             21059..23482
                     /gene="plsB"
                     /locus_tag="OQN13_001914"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCS1615284.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(23637..24509)
                     /gene="ubiA"
                     /locus_tag="OQN13_001915"
     CDS             complement(23637..24509)
                     /gene="ubiA"
                     /locus_tag="OQN13_001915"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCS1615285.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(24522..25019)
                     /gene="ubiC"
                     /locus_tag="OQN13_001916"
     CDS             complement(24522..25019)
                     /gene="ubiC"
                     /locus_tag="OQN13_001916"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCS1615286.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(25242..>25430)
                     /locus_tag="OQN13_001917"
     CDS             complement(25242..>25430)
                     /locus_tag="OQN13_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1615287.1"
                     /translation="DMLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYAL
                     FPIFTASWRATRIKNIGI"
BASE COUNT         6578 a         6544 c         6089 g         6219 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
      601 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
      661 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
      721 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
      781 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
      841 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
      901 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
      961 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcgcggaaga cattatcaat
     1021 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     1081 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     1141 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     1201 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     1261 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     1321 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     1381 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     1441 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     1501 atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg
     1561 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     1621 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     1681 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     1741 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     1801 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     1861 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     1921 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     1981 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     2041 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     2101 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
     2161 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
     2221 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
     2281 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagctc acaaaattaa
     2341 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     2401 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     2461 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcagcttct tagtgccgta
     2521 atgatgtttc tggccagcac atcaacatcc gttttcgtcg gtctgattat gtcgggcttg
     2581 cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg
     2641 gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag
     2701 agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta gaagcctcca
     2761 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
     2821 aacctattgt tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct
     2881 cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact
     2941 gattgccacc ctgcggctgc tgaggctgcc cccaaccgcc ctgcggctga ccaccaccga
     3001 tattaccacc tgccggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc
     3061 caacgttcac cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac
     3121 gggtacgcag ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca
     3181 cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgttct ttcatctcgc
     3241 cggtcgcttt atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac
     3301 catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt
     3361 ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa tcgttctgaa taagtgtaaa
     3421 cgcgcgattg taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa
     3481 caaacctggc cagacattgt tacacaacac tccggataat gcattccaat actgtatatt
     3541 cattcaggtc aatttgtgtc ataattaacc gtttgtgatc gccggtagca ccatgccacc
     3601 gggcaaaaaa gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc
     3661 gcacccataa tctcaaaaac atcaacctcg ttatcccccg cgacaagctc attgtcgtga
     3721 ccgggctttc gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc
     3781 agcgccgtta cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaaa
     3841 agccggacgt cgatcatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga
     3901 cgtctcataa cccgcgttct acggtgggga caatcaccga aatccacgac tatttgcgtt
     3961 tgttgttcgc ccgcgtcggc gaaccgcgct gtccggacca cgacgtcccg ctggcggcgc
     4021 aaaccgtcag ccagatggtg gataacgtgc tgtcgcagcc ggaaggcaag cgtctgatgc
     4081 tgctcgcgcc aatcattaaa gagcgcaaag gcgaacacac caaaacgctg gagaacctgg
     4141 caagccaggg ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc
     4201 cgaaactgga actgcaaaag aaacacacta ttgaagtggt ggttgatcgc ttcaaggtgc
     4261 gtgacgatct tacccaacgt cttgcggagt cgtttgaaac cgcgctggag ctttccggtg
     4321 gtacagcggt agtggcggat atggacgacc cgaaagcgga agagctgctg ttctccgcca
     4381 acttcgcctg cccaatttgc ggctacagta tgcgtgaact ggagccgcga ctgttttcgt
     4441 ttaacaaccc ggcaggtgcc tgcccgacct gtgacggcct tggcgtacag caatatttcg
     4501 atcctgaccg cgtgatccaa aaccccgagc tgtcactggc tggcggtgcg atccgtggct
     4561 gggatcgccg caacttctat tatttccaga tgctgaaatc gctggcagat cactataagt
     4621 tcgacgtcga agcgccgtgg ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg
     4681 gttctggcaa agaaaacatt gaattcaaat acatgaacga tcgtggcgat acctccattc
     4741 gtcgtcatcc gttcgaaggc gtgctgcaca atatggagcg ccgttataaa gagacggaat
     4801 ccagtgcggt acgtgaagaa ttagccaagt ttatcagcaa tcgcccatgc gccagctgcg
     4861 aaggaacgcg tctgcgtcgg gaagcgcgcc acgtgtatgt cgagaatacg ccgctgcctg
     4921 ctatctccga catgagcatc ggtcatgcga tggaattctt caacaatctc aaactcgcag
     4981 gtcagcgggc gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc
     5041 tcgttaacgt cggcctgaat tacctgacac tttcccgctc ggcagaaaca ctttccggcg
     5101 gtgaagccca gcgtatccgt ctggcgagcc agattggtgc gggcctggtt ggcgttatgt
     5161 atgtactgga cgagccgtct atcggcctgc accagcgcga taacgagcgc ctgttgggta
     5221 cgcttatcca tctgcgcgat ctcggtaata ccgtgattgt ggtggagcat gacgaagacg
     5281 caattcgcgc cgctgaccat gtgatcgata tcggtcctgg tgcgggtgtt cacggcggtg
     5341 aagtggtcgc agaaggtccg ctggaagcga ttatggcggt gcctgaatcg ttgaccgggc
     5401 agtacatgag cggtaaacgc aagattgaag tgccgaagaa acgcgttccg gcgaatccag
     5461 aaaaagtgct gaagctgaca ggtgcacgcg gcaacaacct gaaggacgtg acgctcacgc
     5521 tgccagtcgg tctgtttacc tgcatcacag gggtttcagg ttccggtaaa tcgacgctga
     5581 ttaacgacac actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac
     5641 cggcaccgta tcgcgatatt caggggctgg agcatttcga taaagtgatc gatatcgacc
     5701 aaagcccaat tggtcgtact ccacgttcta acccggcgac ctataccggc gtgtttacac
     5761 ctgtgcgcga actgtttgcg ggcgtaccgg aatcccgtgc gcgtggttat acgccaggac
     5821 gtttcagctt taacgtccgt ggcgggcgct gcgaagcctg tcagggcgac ggtgtgatca
     5881 aagtggagat gcacttcctg ccggatatct acgtgccgtg cgatcagtgc aaaggtaaac
     5941 gctataaccg tgaaacgctg gaaattaagt acaaaggcaa aaccatccac gaagtgctgg
     6001 atatgaccat cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcgcgtaagc
     6061 tgcaaacgtt gatggacgtt ggtctgacgt acattcgcct ggggcagtcc gcaaccacac
     6121 tttctggtgg tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg
     6181 ggcagacgct gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc
     6241 aactcctcga cgtgctgcat aaactgcgcg atcagggcaa taccattgtg gtgattgagc
     6301 acaatctcga cgtgatcaaa accgctgact ggattgtcga cctgggacca gaaggcggca
     6361 gtggcggcgg cgaaatcctc gtctccggta cgccagaaac cgtcgcggag tgcgaagctt
     6421 cgcatacggc acgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc
     6481 cttttccttt cagagttgta ccagcaattt acgcttttct tccggtagta aattcactgc
     6541 ctgctgataa gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag
     6601 atacacggtg ctccagtgcg ctttattcag atggcggcta ggacgcacat cgctgtgctg
     6661 ctgacgtagc agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc
     6721 tacttctttc accatcgcaa acagtacatc ttccactttg atctgcgtcg ctttccagtc
     6781 gttatgcacg ctctgttctg cgcctggttt tgccatgcaa tattgtagca actccgaaat
     6841 ggtcattttt tactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg
     6901 atgttccccc agccagatgc cttgccaggt gccggtctga atacgccctt tatgtaacgg
     6961 caatacaagc gatgttccca gcattgagga tttgatatga gaaggcatat cgtctgctcc
     7021 ctcatagtca tgctcataat ttccgttgtc gggaacggtg cggaggaaaa aacgctccat
     7081 gtcgtggcgt acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg
     7141 cagcaacaga tgcagtaagc cgatgttaac gcgcggcata tcagccagct gattcagaat
     7201 ttcatccgtt accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata
     7261 ccacatgtgc tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg
     7321 ataaaggtgt gattaaaaaa acagcattcg ggagagcatc acgctctccc gctctgtcag
     7381 tattctgaat tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag
     7441 gtagagttgg aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta
     7501 atatcgttat cggaatcgcc ataaaaaatt cggatatttt tatcctgcag ccattgcgat
     7561 tttgtatttt gccctggttt atcgcccgca aagattaccg gattcatgtt ggtggcagga
     7621 atatgaaaat tatccgccag cgtttttgaa accgtttctg ttttcgtcgg gctacgacca
     7681 gtcacaaaga agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc
     7741 tcttttggaa tgctgaattc atcccagcca ttattcattt tttcccagaa cacaggattt
     7801 ttcagataat cttcgctttc tggcgagaag gtttttttgc cgcgccagaa ccccggactg
     7861 gaaaaaagta ccgtgtcatc gatatcaaac cccaccgcca ttggcggacg ccctgcgagg
     7921 ctattttcaa tttgtgcgac cgaaacccaa tgaattggtg cctgttcagc aagcctggca
     7981 acgttagtcc cagggttaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt
     8041 agagcgaaca ataagcaaac ggcactgatt gcctgtgtga tcttgcgcat atttttccct
     8101 aaatagtcag tatgttgaaa ctttttattg tgagatttgt tgcaaaaacc atctgaccat
     8161 aacgccagct gccggggaaa ggaaggatta tctgcggttt ttgtgatgaa aagcaggatg
     8221 attatattta acccagtaaa tacgttcctg tctttttttg tataaaaaat gtttattact
     8281 cgatgtaagg atacattaat tttacgttta ttatgatgaa tcctctgcaa atgagcgaaa
     8341 tttgaaagaa catttatctg ctgtacgatt taatgaactt aatgatacag cattacaaaa
     8401 acaatcgaag tttataaaga tgattcctga ttgaccagaa ccctatccta caatggtgac
     8461 taattttatt actcccgatg gagatgatga tatgaatata tcatatgtga atagcaacaa
     8521 aacgacatct ttacctgttg agctagatgc actaaataat aaagacatct cttatgccaa
     8581 agatttttct tatgctaaag atttcttttt atacatagaa acacagttaa aaattgcaaa
     8641 agatttttgt agacctggag aagaagtatc aagttctatt gcaagtaaag tttttcacgc
     8701 atttatcgat ttagttaaca aaatcagggg taagaaagac tttatgtata tttgcacgct
     8761 ttgctgtttt gccgaggagg ttaaaggtga ttattctcat tacaggacct ttttatttga
     8821 tattggtaat caatataagg ttaaacttac acaaagcgga aaaaaagagt tctctttaac
     8881 attagaattt aacgatacta taattgaatc tcagaaagtc acaggcaata aggcaaagta
     8941 tattcttgag gatatagaaa tattctatcg taacaaaccc gatacttatt attaaattta
     9001 ataatggtta gatgtgcaga aattattact ccacacgaaa aaatagggaa aggaattcca
     9061 gataattggg ataaacttaa caataaaaga actgacaacg ttttattatc tctcacgtag
     9121 aacaacggca tcaaaaaaat catctacaaa gaaatagtga aagcgacgac agtttaattt
     9181 cactgcaggc tgggtaactc cagcctgctt tcctgcatta catcaccgca gcaaacgcct
     9241 ttgccacacg ttgtacattt gccgtattta acccggcgac acacatgcga ccgctggcga
     9301 tgagatagac accaaattct tcacgtagtc ggtcaacctg agcggcactt aaaccggtat
     9361 aactgaacat gccgcgctga ttaagcagat aatcgaaatt gcgttctggc atctctgtgc
     9421 ttaatacctt caccaattcc tgacgcattg ccagaatgcg agtacgcatc tcttctactt
     9481 ccgccagcca gctggctttc aatgcctcgt cattcagcac tgcagccacc acctgcgcac
     9541 caaaattcgg cgggctggag tagttgcggc gaactgttgc tttcaattgc cccagtacgc
     9601 ggccagcggc ttcggcatct tcacacagaa cagaaagtcc gccgacgcgc tcgccgtaaa
     9661 gggagaaaat tttcgagaac gaattgctca ccagagcggg taatccagcg ctggcaatgg
     9721 cgcgaatagc gtaggcatcc tcttccatac cggcactaaa tccttgatag gcaatatcga
     9781 ggaatgggat aagctcgcgg gctttgagaa tttcaatcac cgcatcccac tgatcattag
     9841 tgagatcggc acccgttggg ttgtggcaac atggatgcag caacacaata ctgcgggcag
     9901 gtaatgtttt cagcgtcgcc aacaggtcat taaagcgcac gctgttagtc gcttcgtcat
     9961 accaggggta agtactcact tcgaatccag ccccggcgaa tattgctacg tggttttccc
    10021 aggtaggatc gctgacccag acgcctgatt ccgggaagta gcgtttcagg aaatccgcgc
    10081 ccactttcaa tgcccctgag ccgccaaggg tttgaatggt tgctacgcgc tgttgttgca
    10141 gtaccggatg gtccgcacca aacagcagcg gcgcaatggc atggcgatag ctgttaagcc
    10201 cttccatcgg taaataaagc gaagcgccat ggggctgcgc attcaggcgc gcttccgcct
    10261 ccgccacggc tttcagttgt ggaataattc cgtcttcgtt gtagtacaga ccgatactta
    10321 aattcacttt gtcgctgcga gggtcttctt taaaacgctc cataagcgta agaatcgggt
    10381 cgccagcgta ggcgtcaact ttttgaaaca cgcgatggtt ctccaggttt acgggcaggt
    10441 ggttaaaaca caataaaccg gaagaaggcg aagatcgagt ggatgttcag gagcgaacgg
    10501 caattagcaa cagagtgaga ctcatgacaa acgaacatcc gccagaggca cgacctttat
    10561 aagagcatga aagaacatca acttattgaa tttttaggat tttattggcc ggataaggca
    10621 ttcacgccgc atccggcaca gacaatcaaa tattacagaa cgattaatcc acgtatttca
    10681 tcgcgaccct tgaagtcagg cgcgtaataa gttcgtaagc gctcactttt gtcatttcag
    10741 cgatacgttc tacgggcaaa ccttcgcccc ataaaatgac cggatccccg gctttgtcct
    10801 gcgcctgtgg acctaagtct acgcagatca tatccatcgc cacgcgtccg acaatcggta
    10861 cttcgcgacc gttcaccagc actggcgtac cggatggagc ggcgcgcgga taaccatcgc
    10921 catagcccat cgcgactacg ccaagacggg tatcacgttc gcttacccag gttccaccat
    10981 aaccaacagg ctctccggct ttatgctcac gcacggcaat caggctggag gttagtgaca
    11041 tcactggctg acagccaaaa tcggcaccga tggagcgatc ttccagcggc gagacgccat
    11101 aaagaatgat gcccgggcgc acccagtcaa aatgcgattg tggccacagc agaatgccac
    11161 ccgacgcggc aatggaacgt tgaccaggtt tgccttcgca aaaggtatta aagatagcga
    11221 gttgtttctc ggttgcgcca cattttggtt catccgcgcg cgcaaaatgg ctgacgatat
    11281 tcaccggctg acgaacgttt ttgcactgtg tcaggcgatg ataaaacgcc tcagcctgtt
    11341 ccggccttac gcccagacgg tgcataccgg tatcgagttt catccagacg gtaaccggct
    11401 cgtccaggct agcctcttcc agcgcagcca gctgttcttc gttatgcacg gcggtatgaa
    11461 aatgttgcgc agaaatcgtc ggcagatctc tggcatcaaa aaagccttcg agtaacagta
    11521 caggtttggt gattcccccc gcacgcagtc gcagagcttc ttcgagacgg gctacgccaa
    11581 aggcgtcagc atcggggagc gttcgcgcgg tctcaagaag accgtgacca taagcgttcg
    11641 ctttcaccac cgcaaccatt ttactggcag gggccagttc acgaagacgt tgcaggttgt
    11701 gtcgcagagc gcggcggtta atcacaacag ttgccgcttg catttgtgtt ccttgataag
    11761 tgtttgcttt aattacctaa ttcataaaat aattattatt cgtcgtcgta ctgcggcccc
    11821 gcatagttgt cgaagcgcga ccattgaccg ttaaaggtca ggcgtaccgt cccgattggg
    11881 ccgttacgtt gtttaccgat aataatttcc gcgatgcctt ttaaatcact gttttcgtga
    11941 tacacctcat cacgatagat aaacataatc aagtccgcat cctgctcgat agagccagat
    12001 tcacgcaggt cggagttgac cgggcgtttg tcggcacgtt gttccagaga acggttcaac
    12061 tgggacagcg ccaccaccgg cacgttcagt tctttcgcca gtgctttcag cgagcgagag
    12121 atttctgcaa tttccagcgt acggttatcg gaaagcgccg gtacgcgcat cagttgcagg
    12181 tagtcgatca tgataagccc gatgccgccg tgttcacggg caatacggcg tgcgcgggaa
    12241 cgcacttccg ttggcgtcag gccggaggag tcatcgatat agatattgcg tttttcgagc
    12301 aaaataccca tggtgccgga aatgcgcgcc cagtcttcat catcgagctg cccggtacgg
    12361 attttagtct ggtcaacgcg cgacagcgac gccagagaac gcatcataat ctgttctgaa
    12421 ggcatctcca gcgagaagat aagtactggt ttatcctgca acatcgccgc gttttcgacg
    12481 aggttcatcg caaatgttgt tttacccatc gacggacgcg cggcgacgat gatcaaatcc
    12541 gacggctgca agccagcggt ttttttgttg agatcgtcat aaccggtgtt tactccggta
    12601 acgccatcgt gtggctgctg aaacaactgc tcaatacgcg ccacggttgc gtcgagcaca
    12661 tcggcgatgt tcttcggccc ttcgtctttg tttgcacgac tttcggcaat tttaaagacg
    12721 cgggattcag caaggtccag cagatcttcg ctggtacgcc cctgcggatc aaaaccggct
    12781 tcagcaatct cattcgcaac cgagatcatc tcacggacaa cggcacgttc acgcacgatg
    12841 tcagcataag cactgatgtt cgccgcactt ggcgtatttt ttgacagctc tgccagataa
    12901 gcaaaaccac cgacgctatc gagttgcccc tggcgttcca gcgattccgc aagggtaatc
    12961 agatcgatag gactaccgct ttcctgcaaa cgcgccattt cagtaaagat atggcggtgt
    13021 gggcgggtgt aaaaatcgtc tgccaccaca cgctcggcta catcgtccca gcgttcgtta
    13081 tccagcatta aaccgcccaa caccgactgc tccgcttcga tcgagtgcgg aggcactttc
    13141 agcccggcaa cttgtggatc gcgttcgcgg ggttcagcct gctgtttgtt gaagggttta
    13201 tttcctgcca tagtgaatgg agttaccgag ataaagaatg ggtcgaaact ttaccatatg
    13261 aagcagaccc tgacgatacg ttctggagga cacatggcaa cacgaattga atttcacaag
    13321 cacggtggcc cggaagtact tcaagccgta gagttcactc ctgccgatcc ggcagagaat
    13381 gaaatccagg tcgaaaataa agccatcggc atcaatttta tcgacacgta tatccgcagc
    13441 ggcctttacc cgccgccatc actacccagc ggattaggta ccgaagcggc aggcatcgtg
    13501 agtaaagtcg gcagtggtgt aaagcatatt aaggcaggcg atcgtgtagt ctatgcgcag
    13561 tcggcgttag gcgcttacag ctctgtgcat aacattaatg cggataaagc ggcgattctg
    13621 cctgcggcaa tttcttttga gcaagctgcg gcatccttcc tgaaaggctt aacggtttat
    13681 tatctgctgc gcaaaaccta tgaaattaaa cccgatgaac agttcctgtt ccacgcagcg
    13741 gctggcggcg ttggcttaat tgcctgccag tgggcaaaag ccctgggcgc gaaacttatc
    13801 ggcaccgtag gaaccgcgca aaaagcgcag agcgcgctaa aagcgggcgc gtggcaggtt
    13861 attaactatc gtgaagagga tctggtcgag cggttaaaag agatcaccgg cggtaagaaa
    13921 gtgcgcgtgg tgtacgattc cgtgggcaga gacacctggg aacggtcgct ggattgcctg
    13981 caacgccgcg gcttaatggt cagttttggc aactcatcag gtgcggttac cggtgtgaac
    14041 ttaggcattc tcaatcaaaa aggctcgttg tatgtgacac gcccttccct gcaaggctat
    14101 atcaccacgc gggaggaatt aaccgaggcc agtaatgaac tgttctcttt gattgccagc
    14161 ggtgtgatta aggtcgatgt cgccgagcag cagaaatatc cgctgaagga tgcgcagcgt
    14221 gcgcatgaga ttctggaaag ccgggcgacg caaggttcca gcctgttgat tccataaaag
    14281 aaatagggct tccacctggg aagccctttc tttttatagt tcggctgtat gtagggtaca
    14341 gcacgatgaa tctgttagag gcgcaatagt gacagatttg attatcaatt cctattttgt
    14401 tctaaggata aaaccttagg ttgtgatcat ccgcacaatc ccttagtaac gccagcggtc
    14461 ataacgctga tatttcggca cttttggtgc tttaatcgcc ttaataaccc acaccaccgc
    14521 aatcgccagt agtaaccacg gcagcaactt aatcatcaat gccagcatac cgccgaggaa
    14581 cataatggcc gtcgccacaa ccagcgcggc gataatgccc agcaacgaaa cgccggtgac
    14641 catcagcatg acaaaaaagc caatcacaaa aagtagttcc agcatgatgc tctcccaaat
    14701 atgaaatctc ttgctggcat tacaagaatc atgccaaaaa taatctattg atttaacagc
    14761 aaaacgcccc gcgacggtgc gcagggcgtg gtgaatttga ctactttttg gtgaaaagtt
    14821 aacgcttatc cgccaccagt ttgagcgcgt gttccagcac attaatgtct gcacccgctt
    14881 tatgggcatt ttcacttaaa taacgccgcc actgccgcgc gccaggaata ccctggaaca
    14941 agcccaacat atgccgggta atatggccga gatacgtccc ctggctgagt tcacgctcaa
    15001 tgtacggata catggcgcgc actaccgcca ccggatcggc atcggtatcc gaggaaccaa
    15061 agatctcccg gtctaccgcc gccagaatac ccggattctg atacgcctcg cgcccgacca
    15121 tcacgccatc catatgttgc aggtgtgctt tggcctcttc cagcgacttg ataccaccgt
    15181 taatcgacat tgtcagatgc ggaaagtcac gcttcagttg atacacacgc ggataatcga
    15241 gcggcgggat ttcacggttt tctttcgggc ttaaccccga aagccaggct ttacgtgcgt
    15301 ggatgataaa catctcacac tcgcctttgc cggaaacggt gttgatgaaa tcgcagagaa
    15361 attcatagct gtcctggtca tcgatgccaa tacgcgtttt caccgtcact ggaatcgaca
    15421 ccacatcgcg catcgctttc acgcagtcgg caaccagctg cgcattaccc atcagacacg
    15481 caccaaacat gccgttctgc acccggtcag acgggcagcc gacattcagg ttgatctcat
    15541 catatccacg cgcttctgcc agcttcgcac actgtgccag cgccgccgga tcgctaccgc
    15601 cgagttgcaa cgctaccgga tgttcttctt cactgtacgc caggtaatca cctttaccgt
    15661 gaataatcgc ccctgtggtc accatttcgg tatacagcaa cgtattgcgg gaaagcagac
    15721 gcaagaaata gcggcaatgt ctgtccgtcc agtcgagcat tggtgcaacg ctaaaccgac
    15781 cactccagtg aacgtcagtt ttttcaggca ttgcgctggt ttggttgatt ttttgcattt
    15841 cagaattacc gtgcattttc aaatgtagag attattttat cgatatatca tggggttatg
    15901 ttattcagca tcactattca ggaggctcaa tagcggggta ctataccata acaacaggaa
    15961 gcgcctgtct cattgcaaaa gaaaattgag atcctctcaa ggcatgaagc tctcacgaag
    16021 tgatggaaat aatcttatta gccgttaact ttgttaaggc caatgataaa caatccaggt
    16081 tcgacgataa ataaaaaatc acacattaaa ctctggtgat atatctccct gctaatagca
    16141 ttgatagaga aaaaagaacc caataaatat tgggtccttt tatataatgc cttccatact
    16201 atcgaagaac ttcacatatt atttctccga tttaaccccg aacaaatcat aaattaattt
    16261 agaagtgtct gtaagtattt taatctcttc ctttgaggtt gggtcaaacg acttcgcgaa
    16321 gctaattaat tgtggtgcag catctcgcat tttgcttaaa atatcaggat cgagcgttcc
    16381 ttcattcacc agatgtgaca tcttatccag atagccatca aaattcattt ctcccccatc
    16441 agccagacgc ttcatctctc ccaggtactt cttcatatca cgttgagata atttttcaaa
    16501 ctgagctttc aggtagctct cattattttc attaatatac actgtttctg ataaatctgc
    16561 gtaggcacta gaatatgaaa gtgaaattaa cagtgaagca aataatttta agctgttgtc
    16621 atcatgttcc tggcaggcat taacaaacgt taaaaaacca gaacctattg cttgaaaatg
    16681 tatattcgct aaaggttcat tatccttaga ttcctcataa aatagctgaa ccgtggaggg
    16741 aaggccattc ttagtttcta catcaaaagt acattgttca atgggagtcg catctttatt
    16801 atccccagga ttacatatac tattaatcgc gaaatgaaaa atagcccggt caacaactga
    16861 tgcaataaag tcacgatcgt ttaaatcctc atcagtgcaa tcgtcaatat acttattgac
    16921 ccacattttg agatcattta tttgctcaat taaaaacaca tttttttacg tgcatgccat
    16981 aaattatgcg tgattcgttg aataaacaaa tctatttttg caataattat atcccgatga
    17041 cgcagcgctg cataatcaat agatttacca tatatattat ttaatttacc tttgattgaa
    17101 cctctattat ctaatttatc tttgattgca tcttcattat cgaattgatt tgaattgagt
    17161 gttttattaa agtatccagt tgcaccgggt attatcttca acatgattca tctacctttt
    17221 ttgtcgtacc gtgtcctcct tataattctt ttgccatctg gtcactatca aaaatcgctc
    17281 ataatttctg aactacaggt atgcgtttaa acccagttaa ataaatagcg agttaacttg
    17341 aagcaattta tttaaaaatg aattattaat tattatcgtg ttgataatgg tacgacaagg
    17401 caacataaca ccttacctac aaaacagaaa atcaacatta cggcaacaat aagggttctc
    17461 ggtgtttttc ctggaacatg gtaaagtaag gacattctta acccccactt tgaggtgccc
    17521 gatggaaaag accacaacgc aggagttatt agcgcaggct gaaaaaatct gcgcgcagcg
    17581 taatgtgcgc ctgaccccac agcgcctgga agtgttgcgc ctgatgagtc tgcaggatgg
    17641 cgctatcagc gcttatgatc tgcttgattt actgcgcgaa gctgaaccgc aagccaagcc
    17701 gccaacggtt tatcgcgcgc tggattttct gcttgagcaa ggttttgtgc ataaggtgga
    17761 atccaccaac agttatgtgc tctgtcatct gttcgaccag cccacgcata cgtcagccat
    17821 gtttatatgc gatcgctgcg gcgcggtgaa agaagagtgt gcagaaggcg tggaagacat
    17881 tatgcatacg ctggcggcaa aaatggggtt tgccctgcgg cataatgtga ttgaagcaca
    17941 tgggctctgt gcggcatgcg tggaagtgga agcgtgtcgt catcctgaac agtgccagca
    18001 tgatcactct gtgcaggtga aaaagaaacc gcgttaagag ggcgtacatc cttgtacacg
    18061 tcgggcagga gggattaatt accagcgata ttcattgcgg gtttcccaat ccacgacctc
    18121 tttttctgcc tgatctttct gataaccata ccgttcctgg attttaccga ccagttgatc
    18181 acgtttacct tcaatgatcg tcatatcatc atcggtcagt ttgccccatt gctctttcac
    18241 tttaccttta aactgtttcc agttaccgcc ggcttcatct ttattcataa tcaagacctc
    18301 atcgttaggt tgtgaatgag agtacgttca cttttcttct gaacgtgaga ttaagtgtag
    18361 tcagcaattt ggcttaggat aatttattca gaatttttaa ccgtcacgtt gcggcaaacc
    18421 aggtaccgtt acgccagtga cgccgccaga tagccgccag agaaagcccg cgcaacgcca
    18481 gaaagacggt tagtgccagc cacaaaccat gattacccag ccacggcagc gtaaggagcg
    18541 tcagcgcaaa acctgcggcg gccaccgcca tactgttacg catttcggcg gcacgcgttg
    18601 cgcctataaa catgccgtcc agcagataac accagacgcc aaccaacggc aaaatcacct
    18661 gccagataag atagcggtca gccagctgct gaatctgggt taacgacgtc agcaacgcaa
    18721 tgatgtgttc ccctgccagc aaataaacca ccgaaaatag taacgctacg atccccgact
    18781 ggcggcacgc tgcccgccag acatccagca actggctacc gtcgcgcgca ccgtatgcct
    18841 gaccggaatg tgcttcaacc gcgtaggcaa aaccatccag cgcataggcg gtaaaggtga
    18901 gtagcgtcat cagaaccgcg tttacagcga taatgtcact ccccagtcgc gcgccaagta
    18961 cggtgatcgc gccgaaacag agttgcaaca acagcgaacg cagcatgata tcgcggttaa
    19021 gcgccagcaa gcgacggaag tttcctcgcc aggcagtttt cgacatttcg ccggaaattc
    19081 cgcgtagttt gaggatttta cgcaccatta gcagaccaat cagcaatgtt gcatattccg
    19141 caataaccgt cgccagagcc gcgccctgca cgttcatatg cagccccatc actagccaga
    19201 catccagcac aatgttgagg atattgccga ccactaacaa aattactggc gcacgggcat
    19261 attgcacgcc gagtaaccaa ccgagtaata ccagattcgc cagcgacgcc ggtgcgctta
    19321 accagcggat ttcaagaaag cgccgcgcct gttccaggac tgcttcacta ccgccaacaa
    19381 tatgcagagc cagatcgata atcggtgtac gcagcagcgc aattaacgcc ccagccccca
    19441 acgccaacag caacggttgc accagcgcac gggctaatgc ctgaggattt ttggcaccat
    19501 aagcctgcgc agtcagcccg gtggtgctca tgcgtaaaaa cagcaacagc ataaagagaa
    19561 agctggtcgc cgttgcacca accgccacgc cgcccaaata aaccgggcta tcaagatgac
    19621 caattaccgc cgtatcgacc agtcccagca acggaacggt gatattggag aaaatcatgg
    19681 gtaaggcgag atgccagagt gctttatcag atgaagtgag gaatgccatg cagacaagcc
    19741 tgatgaagag agatgaaaaa caaaccgcga cgcctggcgg catcgcggtc tcagagatat
    19801 gttacagcca gtcgccgttg cgaataaccc caaccgccag cccttcaatg gtgaagctct
    19861 gctgacgaag gtcaacgaca attggtttaa actcgctatt ttctggcaac agttcgactt
    19921 tattaccctg ttttttcagg cgcttaacgg taacttcgtc atcaatacgt gcgacaacga
    19981 cctgaccgtt acgtacatcc tgagttttat gcactgccag caagtcacca tccataatgc
    20041 cgatatcttt catcgacatc ccgctgacgc gcagcaggaa atcagcattc ggcttgaaca
    20101 aggaaggatc gacctgataa tgaccttcaa tatgctgctg cgccagaagc ggttcaccgg
    20161 cagccacacg acctaccagc ggcaaccctt cttcctcttc ctgcaacaga cgaatcccgc
    20221 gtgatgcgcc ggaaacaatt tcaataacgc ctttgcgtgc cagcgccttc agatgttctt
    20281 cagccgcgtt tggggaacgg aaccccaaac gctgcgcgat ttccgcacgc gtcggcggca
    20341 tacctgtctg gctgatgtga tcacggatga gatcaaacac ctcttgttgc ctggccgtta
    20401 acgctttcat tccgccccct gggtgtatat acagttatgc tgtgagtata tacagcaaaa
    20461 ggcgattttg gaaccataaa ctgcacaata aaccagagat ttatcgaaat ctggaagggt
    20521 tatccaaaat gcgaccataa caggatgcac caggttatca cggcgacgat aatcgcaatc
    20581 agcaccgcgg cggatcccat atctttagcg cgtccggaaa gctcatggta ttcagagcca
    20641 attcggtcaa ccactgcttc gatggcgcta ttgagaattt ccacaatcat caccagcatc
    20701 acggagctga taagcagcac gcgggtaatt gcgtccacat ccagccagca ggcgatgacc
    20761 accgccaaca ataccgctac gccttcctga cggaatgccg cttcgttgat ccatgcagcg
    20821 cgtaaacctt tccaggaata gccagcagct ttgataattc gggtgaatcc agtggtatta
    20881 ttggccattg aaagaacctt tttacattat gagcgtcaat atcagtgtac cgttaaatcc
    20941 gatgattcgg gtttgcgtaa cgccgcgcga aacatgagcg gataccacag aatttcccat
    21001 gactttctgc tatccttgcc gcgcatttgc attattaacc agaggcttta catcgtttat
    21061 gtccggctgg ccacgaattt actacaaatt actgaattta ccattaagca tcctggtaaa
    21121 aagcaagtct attccggcag atcctgcccc ggaactgggg ctggatacct ctcgtccaat
    21181 tatgtacgtt ttaccgtaca actcgaaagc agatttgctg acgttacgcg cccagtgtct
    21241 ggctcatgac ttgcctgacc cgttagagcc gctagaaatc gacggcacgc tactgccgcg
    21301 ctatgtgttc attcacggcg ggccgcgtgt attcacctat tacacgccga aagaagagtc
    21361 tattaagctg ttccacgact atctcgattt gcaccgtagc aacccaaatc tggatgtgca
    21421 gatggtgcca gtatcggtga tgtttggtcg cgcgccgggg cgtgaaaaag gcgaagtaaa
    21481 cccgccgctg cgtatgctta acggcgtaca gaaatttttc gccgtactgt ggctcggtcg
    21541 cgacagtttt gtgcgtttct cgccgtcagt ttcgctgcgc cgtatggcgg atgaacacgg
    21601 cacggataaa actatcgcac agaaactggc gcgcgtggcg cgtatgcact ttgcccgtca
    21661 acgtctggct gccgtaggcc cacgtctgcc tgctcgtcag gatctgttta ataagctgct
    21721 cgcctcccgc gccattgcca aagcggtaga agatgaagcg cgcagcaaaa aaatctccca
    21781 tgaaaaagcg cagcagaacg cgattgcgct gatggaagag attgcggcga atttctctta
    21841 cgagatgatc cgcctgactg accgcattct gggcttcacc tggaaccgac tttatcaggg
    21901 catcaacgtc cataacgccg agcgcgttcg ccagctggcc cacgacggtc atgagctggt
    21961 gtatgtgcct tgccaccgca gtcacatgga ctacctgcta ctttcttacg tgctgtacca
    22021 ccaggggctg gtgccgccgc atatcgccgc cgggatcaac ctgaacttct ggccggccgg
    22081 gccgattttc cgccgtctgg gggcgttctt tattcgccgt acttttaaag gcaataaact
    22141 ttattccacc gttttccgtg agtatctcgg cgaactgttc agccgtggtt attccgtcga
    22201 gtacttcgtg gaaggcggtc gttcccgcac ggggcgtttg ctggatccga aaaccggtac
    22261 gctgtcgatg accattcagg cgatgctgcg tggcggcacg cgtccgatta cgctgattcc
    22321 gatctatatc ggttatgagc acgtcatgga agtgggtact tacgccaaag aactgcgcgg
    22381 cgcgacgaaa gagaaagaga gcctgccgca gatgctgcgc ggtttaagca agctgcgtaa
    22441 tctcggtcag ggttacgtca acttcggtga accaatgccg ttgatgacct accttaacca
    22501 gcacgtacca gactggcgtg aatctatcga tcccatcgaa gcggtgcgtc cggcctggtt
    22561 aacgccgacg gtcaataata ttgctgccga tctgatggta cgcattaaca acgcaggcgc
    22621 ggcaaacgcc atgaacctgt gttgtactgc gcttctggca tcacgtcagc gctcactcac
    22681 ccgcgagcag ttaaccgagc aactcaactg ctacctggat ctgatgcgca acgtacctta
    22741 ctccacggac tctaccgttc cttcagccag cgccagcgag cttatcgatc acgcgctgca
    22801 aatgaacaag tttgaagtcg agaaagacac tatcggcgac atcatcattc tgccgcgcga
    22861 gcaagcggtg ctgatgacct actatcgcaa caacattgcg catatgttgg tgctgccttc
    22921 gctgatggcg gcaattgtca cccagcatcg ccacatctcc cgcgacgtat tgatggagca
    22981 cgtcaatgtg ctttacccaa tgctgaaagc ggagctgttc ctgcgctggg atcgcgacga
    23041 gttgccggac gttattgatg cgctggcaaa tgagatgcaa cgtcaggggc tgattaccct
    23101 gcaagatgat gagttgcata tcaacccggc gcattctcgc cccctacagc tgctggccgc
    23161 aggcgcgcgc gaaacgctgc aacgttatgc catcaccttc tggttgttga gtgccaaccc
    23221 gtcgatcaac cgcggtacgc tggagaaaga gagccgcacc gttgcgcaac gtctctccgt
    23281 gctgcacggc atcaacgcgc cggagttctt cgacaaggcg gtgttcagtt ctctggtgct
    23341 gacgctgcgt gatgaagggt atatcagcga tagcggcgac gccgaaccgg cagaaacgat
    23401 gaaggtttat cagttgctgg cggagttgat tacatcagac gtgcgtttga cgattgagag
    23461 tgcgacgcag ggcgaagggt aatcagagag aattgccgga tgcggcgaaa acgccttatc
    23521 cggccttcca taatctgcaa attcaataaa ttgcgatgtg ttgtgtaggc ctgagaagcg
    23581 cagcgcatca ggcaacccag aagaaagccg gatgatcatc cggctttttt acatcatcag
    23641 aaatgccagt aactcattgc cagtcctaaa aacagtacca gaccaacata gttattattc
    23701 ataaatgctt taaagcaggc ttcacgctcg cggttggcaa tcagtttttg ttgataaaca
    23761 aacagcgcgc cagccaccag aattgaccag taatatcccc agcctaagcc atttaactca
    23821 ccgatgatcg ccatcagtgc cagtacgcca atctgcaaaa taccaataat caatttatcg
    23881 tattggccga acaggattgc ggtggattta ataccaatct tcacatcatc gtcacggtca
    23941 accattgcat actgcgtgtc gtaagccacc gcccagagaa tattggcgag gaacattaac
    24001 cagcaactca atggcaccga ctcactcaca gcggcaaaag ccattggaat cgaccagcca
    24061 aacgccgcgc ccagcaccac ttgcgggaga tgggtatacc gcttcataaa cgggtacacc
    24121 cacgccaacg ccagagcggc aatcgacaac agaatggtca tcgtattcag cgtcagcacc
    24181 agtaaaaatg aaatcaatac cagcactaca aacagcgcgc gcgcctcttt ctccgttacc
    24241 gcgccgctgg gaagcggtcg gttcgccgtg cgcttaacat gaccatcaaa cttgcggtca
    24301 gcataatcat tcaccacaca tccggcagcg cgcatcagcc agacacccgc gacaaacacc
    24361 gccaggatcc agagctgggg aacgcccggt gtcgccaccc acaacgccca taatgttggc
    24421 cagagcagca gtaacgcgcc aattggctta tccgtacgca ttaagcgatg aaacgccagc
    24481 agcttattct gcgtcagact ccactccata tttttttcct cttagtacaa cggtgacgcc
    24541 ggtaaaaaca gttctgttag caacagcggt ttaccgctta atcgcaggcg ggaacgtcgc
    24601 ccccacagtc cggcatcacg gcctatctca ataaagtccc gggttaatgt cgatgatgtg
    24661 aacagatagc gtcctagcgg cgttttaccc aatttttgta acgccagctc cggcccgctt
    24721 aacgttgaca caggaacgac ggtacgaccg gcaagccacg gttcaccatc ggcacataac
    24781 aaaatttcac gtaaccagta acgagactct ttcggcagca gcggcagttc ttcggggatt
    24841 tcattctgct cgacaaaccc ttcgcggatc atcgtcacgc tcaccgtttt tccctgctgt
    24901 tcaaaacgtt ttgtcatgga atcttccagc aacagccagt cgagcagttg cgaatccagg
    24961 gcagggatct ctttaaaata gcgcagcgca cgcagttgcg ttaacgcggg gtgtgacatg
    25021 ccgaactctc cgttacataa cgtaaaggca ttgtatcgca gaaaagtgtt tacgggagat
    25081 gtaaagcaaa ttaagtgatc taatgcgcaa caaaaacgca acaatcgcat gaagtggtaa
    25141 aacgctgttg aaccttacgc tttgccgatg cccgtgctgg cgagggcaac cgtaggggca
    25201 tcaccgccca tgatgccagg aacggtgatg ttttccttta cttaaatacc tatattcttt
    25261 atacgagtag ctcgccaact cgcagtaaaa attgggaaaa gcgcatattt accatgagat
    25321 ttggagctaa aaagtgtttc ctgagtaaga ttaataggca acatcttatg tctggattta
    25381 tagatacacg tggaattcca attggtatct tttgtaggat tcaacatatc
//
LOCUS       DAJVRF010000052        25104 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJVRF010000052 DAJVRF010000000
VERSION     DAJVRF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.052"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            194..382
                     /locus_tag="OQN13_001918"
     CDS             194..382
                     /locus_tag="OQN13_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCS1615288.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            394..1146
                     /gene="bcsQ"
                     /locus_tag="OQN13_001919"
     CDS             394..1146
                     /gene="bcsQ"
                     /locus_tag="OQN13_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCS1615289.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1143..1307
                     /locus_tag="OQN13_001920"
     CDS             1143..1307
                     /locus_tag="OQN13_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCS1615290.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1500..3761
                     /gene="bcsA"
                     /locus_tag="OQN13_001921"
     CDS             1500..3761
                     /gene="bcsA"
                     /locus_tag="OQN13_001921"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCS1615291.1"
                     /translation="MNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSLILA
                     LICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRYTST
                     LNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVDIFV
                     PTYNENLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIARTTHE
                     HAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHFFSP
                     DPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSYAVIRRKPLDEIGGIAVET
                     VTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLDNPL
                     TGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFVLPH
                     MIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGGLVE
                     EEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVLGGA
                     VAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQILEGQ
                     KVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWALWQD
                     SYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRHPERS
                     ETAQPSDQALAQQ"
     gene            3772..6111
                     /gene="bcsB"
                     /locus_tag="OQN13_001922"
     CDS             3772..6111
                     /gene="bcsB"
                     /locus_tag="OQN13_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCS1615292.1"
                     /translation="MKRKLFWICAVAMRMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6118..7224
                     /gene="bcsZ"
                     /locus_tag="OQN13_001923"
     CDS             6118..7224
                     /gene="bcsZ"
                     /locus_tag="OQN13_001923"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCS1615293.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7206..10698
                     /gene="bcsC"
                     /locus_tag="OQN13_001924"
                     /pseudo
     CDS             7206..10698
                     /gene="bcsC"
                     /locus_tag="OQN13_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10780..12768
                     /gene="hmsP"
                     /locus_tag="OQN13_001925"
     CDS             10780..12768
                     /gene="hmsP"
                     /locus_tag="OQN13_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCS1615294.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12951..14237
                     /gene="dctA"
                     /locus_tag="OQN13_001926"
     CDS             12951..14237
                     /gene="dctA"
                     /locus_tag="OQN13_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCS1615295.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14457..15953
                     /locus_tag="OQN13_001927"
     CDS             14457..15953
                     /locus_tag="OQN13_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS1615296.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16049..16978)
                     /gene="kdgK"
                     /locus_tag="OQN13_001928"
     CDS             complement(16049..16978)
                     /gene="kdgK"
                     /locus_tag="OQN13_001928"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCS1615297.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17210..17977
                     /gene="pdeH"
                     /locus_tag="OQN13_001929"
     CDS             17210..17977
                     /gene="pdeH"
                     /locus_tag="OQN13_001929"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCS1615298.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18047..20107
                     /locus_tag="OQN13_001930"
     CDS             18047..20107
                     /locus_tag="OQN13_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS1615299.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWFKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIATNIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20289..21611)
                     /locus_tag="OQN13_001931"
     CDS             complement(20289..21611)
                     /locus_tag="OQN13_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCS1615300.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22022..23035)
                     /gene="yhjD"
                     /locus_tag="OQN13_001932"
     CDS             complement(22022..23035)
                     /gene="yhjD"
                     /locus_tag="OQN13_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCS1615301.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23084..23983)
                     /locus_tag="OQN13_001933"
     CDS             complement(23084..23983)
                     /locus_tag="OQN13_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1615302.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24503..>25104
                     /locus_tag="OQN13_001934"
     CDS             24503..>25104
                     /locus_tag="OQN13_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1615303.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5894 a         6365 c         6991 g         5854 t
ORIGIN      
        1 ttagcttgtg aatcaattta gaaggtcaca agacgtgtga ttttagtctt ttcgttttta
       61 actttttctg ttgcgtaacg gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac
      121 aataaaccgg cgaaatgctc acggttagaa ttgtttcatg aacggtacgg ttatttcata
      181 gggatcaagc aaaatgaata acaatgaacc agatactctg cctgatcccg cgataggcta
      241 tatcttccag aatgatattg tggcgttaaa gcaggcattt tcactgcctg atattgatta
      301 tgccgatatt tcccaacgcg aacagttggc cgcggcatta aaacgctggc cgttgctggc
      361 agagtttgcg caacaaaagt aggggattgg tgaatggccg tactgggatt gcagggggtg
      421 cggggaggcg tggggacaac aaccatcacc gccgcattag cctggtcatt acaaatgttg
      481 ggagaaaatg tcctggtggt cgatgcctgc ccggacaact tgttgcgcct gtcatttaat
      541 gttgatttta cccaccgtca gggctgggcc agagcgatgc tggatggcca ggactggcgt
      601 gacgctgggt tgcgctacac ctcgcagctc gatttgctgc cttttggtca gttatccatt
      661 gaagaacaag aaaatccaca gcactggcaa acccggctga gcgatatttg ctccggctta
      721 cagcaactaa aagccagcgg gcgttaccag tggattttaa tcgacttacc gcgtgatgcc
      781 tcgcagataa cccaccagct gctgagtttg tgcgatcact cgctggcaat cgtcaatgtg
      841 gatgccaact gccatatccg actgcatcag caagcgctgc cggatggcgc acatattttg
      901 attaatgact tccgtattgg cagtcaggtt caggacgata tttaccagct ttggttgcaa
      961 agccagcgcc gattactgcc gatgctcatt catcgtgatg aagcgatggc tgaatgcctg
     1021 gcggctaagc aaccagtagg tgaatatcgc agtgatgcgc tggcggctga agagatactg
     1081 acgctggcga actggtgcct gttgaactac tccgggctga aaacgccagt cgggagtgca
     1141 tcatgagtat cctgacccgg tggttgctta tcccgccggt caacgcgcgg cttatcgggc
     1201 gttatcgcga ttatcgtcgt cacggtgcgt cggctttcag cgcgacgctc ggctgtttct
     1261 ggatgatcct ggcctggatt tttattccgc tggagcaccc gcgctagcag cgtattcgcg
     1321 cagaacataa aaacctgtat ccgcatatca acgcctcgcg tccgcgtccg ctggacccgg
     1381 tccgttatct cattcaaaca tgctggttac tgatcggtac atcgcgcaaa gaaacgccga
     1441 aaccgcgcag gcgggcattt tcaggtctgc agaatattcg tggacgttac catcaataga
     1501 tgaacgagct gcctgagcgc gttagccata aaacacagca tcttgatgag aaaaaagagc
     1561 tcggtcattt gagtgccggg gcgcggcggt tgatcctcgg tatcatcgtc accttctcgc
     1621 tgattctggc gttaatctgc gttactcaac cgtttaaccc gctggcgcag tttatcttcc
     1681 tgatgctgct gtggggtgta gcgctgatcg tacggcggat gccggggcgc ttctcggcgc
     1741 taatgttgat tgtgctgtcg ctgaccgttt cttgccgtta tatctggtgg cgttacacct
     1801 ctacgctgaa ctgggacgat ccggtcagcc tggtgtgcgg gcttattctg ctcttcgctg
     1861 aaacgtacgc gtggattgtg ctggtgctcg gctacttcca ggtagtatgg ccgctgaatc
     1921 gtcagccggt gccattgccg aaggatatgt cgctgtggcc gtcagtggat atctttgtcc
     1981 cgacttacaa cgaaaatctc aatgtggtga aaaataccat ttacgcctcg ctgggtatcg
     2041 actggccgaa agacaaactg aacatctgga tcctcgatga cggcggcagg gaagagtttc
     2101 gccagtttgc gcaaaacgtg ggggtgaaat atatcgcccg caccactcat gaacatgcga
     2161 aagcgggcaa catcaacaat gcgctgaaat atgccaaagg cgagttcgtg tcgattttcg
     2221 actgcgacca cgtaccaacg cgatcgttcc tgcaaatgac catgggctgg ttcctgaaag
     2281 agaaacagct ggcgatgatg cagactccac accacttctt ctcgccggac ccgtttgaac
     2341 gcaacctggg acgttttcgt aaaacgccga acgaaggcac gctgttctat ggtctggtgc
     2401 aggatggcaa cgatatgtgg gacgccactt tcttctgcgg ttcctatgcg gtgattcgtc
     2461 gtaagccgct ggatgaaatt ggcggcattg ctgtcgaaac cgtgactgaa gatgcgcata
     2521 cttctctgcg tttgcaccgt cgtggctata cctccgcgta tatgcgtatt ccgcaggcgg
     2581 cggggctggc gaccgaaagt ctgtcggcgc atatcggtca gcgtattcgc tgggcgcgcg
     2641 ggatggtaca aatcttccgt ctcgataacc cgctcaccgg taaaggactg aagtttgctc
     2701 agcggctgtg ctacgtcaac gccatgttcc acttcttgtc gggcattcca cggctgatct
     2761 tcctgactgc gccgctggcg ttcctgctgc ttcatgccta catcatctat gcgccagcgt
     2821 tgatgatcgc cctattcgtg ctgccgcata tgatccatgc cagcctgacc aactcgaaga
     2881 tccagggcaa atatcgccac tctttttgga gtgaaatcta cgaaacggtg ctggcgtggt
     2941 atatcgcacc accgacgctg gtggcgctga ttaacccgca caaaggcaaa tttaacgtca
     3001 ccgccaaagg tggactggtg gaagaagagt acgtcgactg ggtgatctcg cggccctaca
     3061 tcttccttgt tctactcaac ctggtgggcg ttgcggtagg catctggcgc tacttctatg
     3121 gcccgccaac cgagatgctc accgtggtcg tcagtatggt gtgggtgttc tacaacctga
     3181 ttgttcttgg cggcgcagtt gcggtatcgg tagaaagcaa acaggtacgc cgatcgcacc
     3241 gcgtggagat gacgatgccc gcggcaattg cccgcgaaga tggtcacctc ttctcgtgta
     3301 ccgttcagga tttctccgac ggtggtttgg ggatcaagat caacggtcag gcgcagattc
     3361 tggaagggca gaaagtgaat ctgttgctta aacgcggtca gcaggaatac gtcttcccga
     3421 cccaggtggc gcgcgtgatg ggtaatgaag ttgggctgaa attaatgccg ctcaccaccc
     3481 agcaacatat cgattttgtg cagtgtacgt ttgcccgtgc ggatacatgg gcgctctggc
     3541 aggacagcta cccggaagat aagccgctgg aaagtctgct ggatattctg aagctcggct
     3601 tccgtggcta ccgccatctg gcggagtttg cgccttcttc ggtgaagggc atattccgtg
     3661 tgctgacttc tctggtttcc tgggttgtat cgtttattcc gcgccacccg gagcggagcg
     3721 aaacggcaca accatcggat caggctttgg ctcaacaatg atgataacgc gatgaaaaga
     3781 aaactattct ggatttgtgc agtggctatg aggatgagtg cgttcccctc tttcatgacg
     3841 caggcgacgc cagcaacgca accactgatc aatgctgagc cagctgtagc cgcccagacg
     3901 gaacaaaatc cgcaggtggg gcaagtgatg ccgggcgtgc agggcgctga tgcgccagtc
     3961 gtggcgcaga acggtccttc gcgtgatgtg aagctgacct ttgcgcaaat tgcgccgccg
     4021 ccgggcagca tggtgctacg tggcattaac ccgaacggca gcattgagtt tggtatgcgc
     4081 agcgatgaag tggtgacgaa ggcgatgctc aacctcgaat acacgccatc gccatcgtta
     4141 ctgcctgtcc agtcgcagtt aaaggtttat ctcaatgatg agctgatggg cgtgctgcca
     4201 gtgaccaaag aacagttggg taaaaaaacg ctggcgcaaa tgcccattaa cccactgttt
     4261 attaccgact tcaaccgtgt gcggctggag tttgtcggcc attatcagga cgtgtgcgaa
     4321 aacccggcca gcaccacgct ttggctggat gttgggcgga gcagtggact ggatctgacc
     4381 tatcagaccc tgaatgtgaa gagtgacctg tcacacttcc cggtgccatt ctttgacccg
     4441 cgcgataacc gtactaacac cttgccgatg gtctttgcgg gtgcgccgga tgttgggctg
     4501 caacaagcat ctgccattgt cgcctcgtgg tttggttcgc gttctggctg gcgtgggcag
     4561 aacttcccgg tgctctataa ccaattgccg gatcgcaatg ccattgtctt tgcaaccaac
     4621 gacaaacggc cggacttcct gcgcgatcat ccggcggtaa aagccccggt gattgagatg
     4681 attaaccatc cgcagaatcc ttacgtcaaa ctgctggtgg tgtttggtcg tgacgacaaa
     4741 gacctgttgc aggcagcgaa aggtatcgct cagggtaaca ttctgttccg tggtgaaagc
     4801 gtggtagtga atgaagtgaa accgctgcta ccgcgtaagc cgtacgatgc gccgaactgg
     4861 gtacgtaccg atcgtccggt cacatttggc gaactgaaaa cctatgaaga acagttacaa
     4921 tccagcggtc ttgagccagc agcgattaac gtttcgctaa acctgccgcc ggatctctac
     4981 ctgatgcgca gtaccggcat tgatatggat attaattacc gctacaccat gccgccggtg
     5041 aaagacagtt cgcggatgga tatcagcctg aataaccagt tcctgcaatc cttcaacctg
     5101 agcagtaaac aggaggcgaa ccgcctgctg ctgcggattc cggtattaca aggtttgctg
     5161 gatggcaaaa cggatgtctc tattccggcg ctgaaactgg gcgcgaccaa ccagctgcgc
     5221 ttcgactttg agtatatgaa cccgatgccg ggcggttcgg tggataactg tattaccttc
     5281 cagccggtgc agaatcatgt ggtgattggt gacgattcca ccatcgactt ctcgaagtat
     5341 taccacttca tcccgatgcc ggatctacgc gcctttgcta acgcgggctt cccgttcagc
     5401 cggatggcgg atctgtcgca aaccatcacc gtgatgccga aaacgcctaa cgaagcacag
     5461 atggaaacgt tgctgaatac tgttggtttt atcggcgcac agacgggctt cccggcgatt
     5521 aatctgacgg tgaccgatga tggcagcacc attcagggca aagatgccga catcatgatc
     5581 atcggtggta tcccggacaa attaaaagac gataagcaga tcgacctgtt ggtgcaggcg
     5641 accgaaagct gggtgaaaac accgatgcgc cagaccccgt tccccggcat tgtgccggac
     5701 gagagcgatc gtgcggcaga aacccagtca acgctgacct cttccggtgc gatggcggcg
     5761 gtgattggct tccagtcgcc gtataacgac cagcgcagcg tgattgcgct gctggcagat
     5821 agcccacgcg gttatgaaat gcttaacgat gcggtgaacg atagcggcaa acgcgccacc
     5881 atgttcggtt cggtcgcggt gatccgcgag tccggtatca acagcctacg tgttggcgac
     5941 gtttattacg taggtcatct gccgtggttc gagcgcttgt ggtatgcgct ggcaaaccat
     6001 ccgattctgc tggcggtgct ggcggcaatc agtgtgatat tgctggcatg ggtactgtgg
     6061 cgtctgctgc gaattattag tcgtcgtcgt cttaacccgg ataacgagta attgaagatg
     6121 aatgtgttgc gtagtggaat cgtgacgatg ctgctgttgg ctgcctttag tgttcaggca
     6181 gcctgtacct ggcctgcctg ggagcagttt aaaaaggatt acatcagtca ggaagggcgc
     6241 gtcatcgccc ccagcgacgc gcgcaaaatc accacctccg aagggcaaag ttacggtatg
     6301 ttctttgccc tggcggctaa cgaccgtgta gctttcgata atattctcga ctggacgcag
     6361 aacaatctcg ctcagggctc tttaaaagaa cgtttgcccg cctggctgtg gggcaagaaa
     6421 gagaacagta agtgggaagt gctggacagc aattcggcct ccgatggtga tgtctggatg
     6481 gcctggtcgt tgctggaggc ggggcgtttg tggaaagagc agcgttatac cgacatcggc
     6541 agcgcgttgc taaaacgtat cgcgcgggag gaagtggtga cggtgcctgg gctgggttcc
     6601 atgttgttac cgggcaaagt gggttttgct gaggataaca gctggcgttt taaccccagc
     6661 tacctgccgc cgacgctggc gcagtatttc acccgctttg gcgcgccgtg gaccacgctg
     6721 cgcgaaacca atcaacgttt attgctggaa accgccccga aaggtttttc gccagactgg
     6781 gtgcgctatg agaaagacaa aggctggcag ctaaaagccg aaaaaacatt gatcagcagc
     6841 tacgacgcta tccgcgttta catgtgggta ggcatgatgc ctgacagcga tccgcaaaaa
     6901 gcgcggatgc tcaaccggtt taaaccgatg gcgacattca ctgagaaaaa cggttatccg
     6961 ccggaaaaag tggatgtggc tacggggaaa gcgcagggta aaggaccgat cggtttttct
     7021 gccgccatgc tgcccttttt acaaaaccgc gatgcgcagg ccgttcagcg ccagcgcgtg
     7081 gccgataact ttcccggcag cgatgcctat tacaactatg tgctgaccct gtttggacaa
     7141 ggctgggatc aacaccgttt ccgcttctcg acaaaaggtg agttattacc tgactggggc
     7201 caggaatgcg caaattcaca ctaaacatat tcacgctttc cctcggtctg gccgtcatgc
     7261 cgatggtcga ggcagcacca accgctcagc aacagttgct ggagcaagtt cggttaggcg
     7321 aagcgaccca tcgtgaagat ctggtgcaac agtcgttata tcggctggaa cttattgatc
     7381 cgaataaccc ggacgtcgtt gccgcccgtt tccgttcttt gttacgtcag ggcgatattg
     7441 atggcgcgca aaaacagctc gatcggctgt cgcagttagc gccgagttca aatgcgtata
     7501 aatcgtcgcg gactacgatg ctactttcca cgccggatgg tcgtcaggca ctgcaacagg
     7561 cacgattgca ggcgacgacc ggtcatgcag aagaagctgt ggcgagttac aacaaactat
     7621 tcaacggtgc gccgccggaa ggtgacattg ctgtcgagta ctggagtacg gtggcgaaaa
     7681 ttccggctcg ccgtggcgaa gcgattaatc agttaaaacg catcaatgcg gatgcaccgg
     7741 gcaatacggg cctgcaaaac aatctggcgc tattgctgtt tagtagcgat cgccgtgacg
     7801 aaggttttgc cgtcctggaa cagatggcaa aatcgaacgc cgggcgcgaa ggggcctcta
     7861 aaatctggta cgggcagatt aaagacatgc ccgtcagtga tgccagtgtg tcggcgctga
     7921 aaaaatatct ctcgatcttt agtgatggcg atagcgtggc ggctgcgcaa tcgcaactgg
     7981 cagaacagca aaaacagctg gccgatcctg ctttccgcgc tcgtgcgcaa ggtttagcgg
     8041 cggtggactc tggtatggcg ggtaaagcca ttcccgaact acaacaggcg gtgcgggcga
     8101 acccgaaaga cagtgaagct ctgggggcgc tgggccaggc gtattctcag aaaggcgatc
     8161 gcgccaatgc agtggcgaat ctggaaaaag ccctcgcact ggacccgcac agcagcaaca
     8221 acgacaaatg gaacagtctg ctgaaagtaa accgctactg gctggcgatc cagcagggcg
     8281 atgctgcgct gaaagccaat aatcctgacc gggcagaacg cctgttccag caggcgcgta
     8341 atgtcgataa caccgacagt tatgcagtgc tggggctggg cgatgtggcg atggcgcgaa
     8401 aagattatcc cgccgccgaa cgttattatc agcagacctt gcgtatggac agcggcaaca
     8461 ctaacgccgt gcgcgggctg gcaaatattt accgccagca atcgccagaa aaagctgaag
     8521 cgtttatcgc ctcgctctct gccagtcagc ggcgtagcat tgatgatatc gaacgcagcc
     8581 tgcaaaacga ccgtctggca cagcaggcag aggcactgga aaaccagggc aaatgggcgc
     8641 aggcggcagc acttcagcgg caacgactgg cgctggaccc cggcagcgta tggattactt
     8701 accgactttc gcaggatctc tggcaggccg gacaacgcag ccaggccgat acgttaatgc
     8761 gcaatctggc gcagcagaag tcgaacgacc cagagcaggt ttacgcttac gggctgtacc
     8821 tacgcttacg ggctgtacct ctctggtcat gaccaggaca gagcggcgct ggcgcatatc
     8881 aacagcctgc cgcgtgcgca gtggaacagc aatattcagg agctggttaa tcgactgcaa
     8941 agcgatcagg tgctggaaac cgctaaccgc ctgcgagaaa gcggcaaaga ggcagaagcg
     9001 gaagcgatgc tgcgccagca accaccttcc acgcgtattg acctcacgct ggctgactgg
     9061 gcgcaacaac gacgtgatta caccgccgcc cgcgctgcat atcagaatgt cctgacgcgg
     9121 gagccagcta acgccgacgc cattcttggt ctgacggaag tggatattgc tgccggtgac
     9181 aaagcggcgg cacgtagcca gctggcgaaa ctgcccgcta ccgataacgc ctcgctgaac
     9241 acacagcggc gcgtggcgct ggcacaggcg cagcttggcg ataccgcagc agcgcagcgg
     9301 acgtttaata agttgatccc gcaggcaaaa tctcagccac cgtcgatgga aagcgcgatg
     9361 gtgctgcgtg atggtgcgaa gtttgaagcg caggcgggcg atccaacgca ggcgctggaa
     9421 acctacaaag acgccatggt cgcatccggt gtgactacga cgcgtccgca ggataacgac
     9481 acctttaccc gactgacccg taacgacgag aaagatgact ggctgaaacg tggcgtgcgc
     9541 agcgatgcgg cggacctcta tcgccagcag gatcttaacg tcacccttga gcacgattac
     9601 tggggttcga gcggcaccgg tggttactcc gatctgaaag cgcacactac catgttgcag
     9661 gtggatgcgc cgtattctga cgggcggatg ttctttcgca gtgatttcgt caatatgaac
     9721 gtcggcagtt tctccactaa tgccgatggc aaatgggatg acaactgggg cacctgtaca
     9781 ttacaggact gtagcggcaa ccgcagccag tcggattccg gtgccagcgt ggcggtcggc
     9841 tggcgaaatg acgtctggag ctgggatatc ggtaccacgc cgatgggctt caacgtggtg
     9901 gatgtggtcg gcggcatcag ttacagcgat gatatcgggc cgctgggtta caccgttaac
     9961 gcccaccgtc ggcccatctc cagttctttg ctggcctttg gtgggcaaaa agactccccg
    10021 agcaataccg ggaaaaaatg gggtggcgta cgtgccgacg gtgtggggct aagtctgagc
    10081 tacgataaag gtgaagcaaa cggcgtctgg gcatcgctta gtggcgacca gttaaccggt
    10141 aaaaatgtcg aagataactg gcgcgtgcgc tggatgacgg gctattacta taaggtcatt
    10201 aaccagaaca atcgccgcgt cacaatcggc ctgaacaaca tgatctggca ttacgacaaa
    10261 gatctgagtg gctactcact cggtcagggc ggttactaca gtccgcagga atacctgtcg
    10321 tttgccatac cggtgatgtg gcgggagcgc acggaaaact ggtcgtggga gctgggggcg
    10381 tctggctcgt ggtcgcattc acgcaccaaa accatgccgc gttatccgct gatgaatctg
    10441 atcccgaccg actggcagga agaagctgcg cggcaatcca acgatggcgg cagcagtcag
    10501 ggcttcggct acacggcgcg ggcattactt gaacgacgtg ttacttccaa ctggtttgtt
    10561 ggcacggcaa ttgatatcca gcaggcgaaa gattacgcac ccagccattt cctgctctac
    10621 gtacgttatt ccgccgccgg atggcagggt gacatggatt taccgccgca gccgctgata
    10681 ccttacgccg actggtaagt tttcagatag cgcctctctt aatgccgctg cgatcgggta
    10741 tactcgggcg gcaatctggg atttccgggg ggagacaatt tgcgcgtaag tcgctcgtta
    10801 acaatcaagc agatggcaat ggtggcagcc gttgtcctgg tgttcgtttt tattttttgc
    10861 accgttttgc tgttccatct ggtccagcag aatcgctata acacggctac gcaactggaa
    10921 agcattgctc gctctgtccg cgaaccctta tcttcagcta ttttgaaagg cgatattccc
    10981 gaagcggaag ctattcttgc cagcattaaa ccggcaggcg tggtcagccg tgccgatgta
    11041 gtgctgccta accagttcca ggcgctgcgt aaaagtttta ttccagagcg cccggtgccg
    11101 ataatggtta ctcgcctgtt tgagctaccg gttcaaatct cgctgggcgt ttactcgctc
    11161 gaacgtccgg caaacccgca gccaattgcc tatctggtac tacaggcgga ttccttccgt
    11221 atgtataagt tcgtgatgag caccctctca acgttagtga ccatttactt acttttgtcg
    11281 cttatcctga ccgtcgccat cagctggtgc attaaccgcc tgattttgca tccgttacgc
    11341 aatattgctc gcgaacttaa cgccatccca gcccaggagc ttgttggtca ccaactggca
    11401 ttaccgcgtc tgcatcagga cgatgaaatc ggtatgttgg tgcgcagtta caacctcaac
    11461 cagcaattgc tgcagcgcca ttatgaagaa cagaacgaaa atgcgatgcg cttcccggtg
    11521 tcggatttgc cgaacaaagc cttgctgatg gagatgctgg agcaggttgt cgcgcgtaaa
    11581 caaaccaccg cgctgatgat catcacctgt gaaaccctgc gtgatactgc gggcgtgctg
    11641 aaagaggcgc aacgagaaat tctgctgctg acgctggtgg aaaaactcaa atcggtactg
    11701 tcgccacgta tgatcctcgc gcagattagc ggttatgact ttgctgtcat tgccaacggt
    11761 gtacaggaac cgtggcacgc aatcacctta ggtcagcaag tgctcaccat catgagcgag
    11821 cgcctgccga ttgaacgtat tcaactccgt ccgcactgta gcattggcgt ggcgatgttc
    11881 tacggcgatc tcaccgccga acagctttac agtcgcgcta tttctgcggc atttaccgct
    11941 cgccataaag gcaagaatca gattcagttc tttgatccgc agcagatgga agccgcccag
    12001 aagcggttga cggaagagag cgatatcctt aatgcactgg aaaatcatca gtttgctatt
    12061 tggttacagc cacaggtcga gatgaccagc ggtaaactgg tcagtgcgga agtgttactg
    12121 cgtatccagc aaccggatgg cagttgggac ctgccggatg gcttaatcga tcgcattgag
    12181 tgctgtgggc tgatggttac cgtcggtcac tgggtgctgg aagagtcctg tcgattgctt
    12241 gcagcctggc aagagcgcgg cattatgctg cccttgtcgg taaacctctc tgcgctgcaa
    12301 ctgatgcacc caaatatggt ggcggatatg ctggaactgt taacccgcta tcgcattcag
    12361 ccgggaacac tgattctgga agtgacagaa agccgacgta ttgacgaccc tcatgctgcg
    12421 gtggcaatcc tccgtccgct gcgcaatgcc ggagttcggg tggcgctgga tgatttcggc
    12481 atgggctacg cagggctgcg tcagctgcag catatgaaat cgttgccaat cgacgtactg
    12541 aaaatcgaca aaatgtttgt tgaaggcttg ccggaagata gcagcatgat tgctgcaatt
    12601 atcatgctgg cgcagagcct gaacttacaa atgattgccg aaggcgtgga gactgaagca
    12661 caacgcgact ggctggcaaa agcgggcgtt ggtattgccc agggcttcct ttttgctcac
    12721 ccactcccta ttgaaatctt cgaagagagt tacctggaag aaaagtagct accccaaact
    12781 gattacaaaa ctttaaaaag tgctggtttg tgcgagccag ctcaaacttt ttaacctttt
    12841 tgtttcaatt atgatccagg tacatttctg tgatgttgtc tgggtgttat tttaaggccg
    12901 caggtacccc ataaccttac aagacctgtg gttttactaa aggacaccct atgaaaacct
    12961 ctctgtttaa aagcctttac tttcaggtcc tgacagcgat agccattggt attctccttg
    13021 gccatttcta tcctgaaata ggcgagcaaa tgaaaccgct tggcgacggc ttcgttaagc
    13081 tcattaagat gatcatcgct cctgtcatct tttgtaccgt cgtaacgggc attgcgggca
    13141 tggaaagcat gaaggcggtc ggtcgtaccg gcgcagtcgc actgctttac tttgaaattg
    13201 tcagtaccat cgcgctgatt attggtctta tcatcgttaa cgtcgtgcag cctggtgccg
    13261 gaatgaacgt cgatccggca acgcttgatg cgaaagcggt agcggtttac gccgatcagg
    13321 cgaaagacca gggcattgtc gccttcatta tggatgtcat cccggcgagc gtcattggcg
    13381 catttgccag cggtaacatt ctgcaggtgc tgctgtttgc cgtactgttt ggttttgcgc
    13441 tccaccgtct gggcagcaaa ggccaactga tttttaacgt catcgaaagt ttctcgcagg
    13501 tcatcttcgg catcatcaat atgatcatgc gtctggcacc tattggtgcg ttcggggcaa
    13561 tggcgtttac catcggtaaa tacggcgtcg gcacactggt gcaactgggg cagctgatta
    13621 tctgtttcta tattacctgt atcctgtttg tggtgctggt attgggttca atcgctaaag
    13681 cgactggttt cagtatcttc aaatttatcc gctacatccg tgaagaactg ctgattgtac
    13741 tggggacttc atcttccgag tcggcgctgc cgcgtatgct cgacaagatg gagaaactcg
    13801 gctgccgcaa atcggtggtg gggctggtca tcccgacagg ctactcgttt aaccttgatg
    13861 gcacatcgat atacctgaca atggcggcgg tgtttatcgc ccaggccact aacagtcaga
    13921 tggatatcgt ccaccaaatc acgctgttaa tcgtgttgct gctttcttct aaaggggcgg
    13981 caggggtaac gggtagtggc tttatcgtgc tggcggcgac gctctctgcg gtgggccatt
    14041 tgccggtagc gggtctggcg ctgatcctcg gtatcgaccg ctttatgtca gaagctcgtg
    14101 cgctgactaa cctggtcggt aacggcgtag cgaccattgt cgttgctaag tgggtgaaag
    14161 aactggacca caaaaaactg gacgatgtgc tgaataatcg tgcgccggat ggcaaaacgc
    14221 acgaattatc ctcttaatct caccacttat gcccgcactc ccttccccga gtgcgggtgt
    14281 ttgcgcataa ttgacccctg tgcctgaact taccacctgg gtgagttcag gtttatttca
    14341 cttcttccgt gacattttca tgttcttgcg gtctaacacg aagtgttttt aacgtcattt
    14401 agactgactg acaacgggca gttttttatt accaggatag ttgatcaggg gttcacatgc
    14461 agggcacaaa aattcgactt ttagcgggcg gtttgctgat gatggccact gctggctatg
    14521 tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt tccaacggtt
    14581 tacagtggca agtgctgacc accccccagc gtcccagcga tcgtgttgaa attcgcctgc
    14641 tggttaatat cggttcgctc gccgaaagta cacaacagag cggttacagt cacgccatcc
    14701 ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc acaggcgcgt tcattgtggc
    14761 agcaggggat cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat gacaccacgc
    14821 tgtttaatct gagtttgccc aataaccgta acgatttgct gaaagaagcg ctctcttatc
    14881 tggcaaatgc cactggcaaa ttgaccatca caccagaaac catcaaccac gcgctgcaaa
    14941 gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg cgctatcgtc
    15001 tgaaagggtc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa cccgttgaag
    15061 cggaaaaaat taaagatttc tatcagaaat ggtacacccc ggatgcaatg acgctactgg
    15121 tggtgggaaa cgtggatgcg cgctcggttg tcgaccaaat caacaaaacg tttggcgaac
    15181 tgaaaggcaa acgtgaaacg ccagctccgg tgccgacgct ttctccgctg cgtgcggaag
    15241 cggtgagtat tatgactgac gcggtgcgtc aggaccggtt atctatcatg tgggatacgc
    15301 cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg gacctggccc
    15361 gcgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtaacagc aaagacatcg
    15421 gtcttggatt tgactgccgt gtgctgtatc tgcgtgcgca gtgtgccatc aacatcgaat
    15481 caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg gcgaaggttc
    15541 gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag aaactggagc
    15601 tgcagaaact gtttgccgcc tatgcgcggg ctgataccga tattctgatg ggtcagcgga
    15661 tgcgttcgct gcaaaatcag gtcgtcgata tcgcgccgga gcagtatcag aaactgcggc
    15721 aggatttcct taacagcctg acggtggaga tgttaaatca ggatctgcgt cagcagttgt
    15781 cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt aacatgaaag
    15841 cgttgcaggc ggcctgggat caaatcatgg ccccatcgac tgctgctgcc gccacctctg
    15901 tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgcacag taatgcctga
    15961 tggtgatgag ttagcacaac catcaggtat ttatggatga gctggataga ggggtaacac
    16021 tttatccagc cttttgcata tgctgcgttt acgctggcat cgcctcacgc gggataatcg
    16081 cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgcc gcgttttccg
    16141 cactgccgcc cgtcaaacat accgccagat aaccggcgct gaaagagtcg ccagctgcgg
    16201 tggtatcgat cactttttct ttcggcagtt tcaccgccgg aacatccact aacccttcgc
    16261 cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct ttcacgcccg
    16321 cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc gcgtcttcat
    16381 cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgttggtac acctgctgtg
    16441 tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc acttttccgc
    16501 cgttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc gggcttaaga
    16561 tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct tcgcaaatcg
    16621 ccgcagactg ctcactctcc agccagaatt tggcggcggc ttcgttccgc cagtagtaga
    16681 acgtacgctc gccggtgctg tcggtttcaa tgtagtaaag gcccggcaga cggttttcca
    16741 tccgttgggt cagggaagta tcaacgttct cgccgtgcca ggcgtccagc atttgctggc
    16801 taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca ggatcgacct
    16861 gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc ttaacgtccg
    16921 cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt ttggacatag
    16981 tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt tgctggtcaa
    17041 ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg aaagattccg
    17101 ttgtggagtg aggaaattta ccccggccag gcggtaaagt tctgcccttg cgcgccgata
    17161 atctttgtcg agtccgggca gcatcacttt taaacacagg acatctttga tgataaggca
    17221 ggttatccag cgaataagca accctgaagc aagcatcgag agcttgcagg aacggcgttt
    17281 ttggttgcag tgtgagcgtg cttacacctg gcagccgatc tatcaaacat gcggacggtt
    17341 aatggccgtg gagctattaa cggtggtcac gcatcccttg aacccttcgc aacgcctgcc
    17401 gccggatcgc tattttactg aaatcaccgt cagccatcgg atggaggttg tgaaagagca
    17461 gattgatttg ctggcgcaaa aagccgactt ctttgtagag cacgacctgc tggcatcggt
    17521 caatattgat ggccctacgc tcatcgccct gcgtcagcaa ccaaaaatcc tgcgccaaat
    17581 tgagcgtctt ccctggctgc gtttcgaact ggtggaacac atccgcctgc cgaaagattc
    17641 aacctttgcc tcgatgtgtg aatttggccc gctgtggctg gatgattttg gcaccgggat
    17701 ggcaaatttc tctgcgctaa gtgaagtgcg ttatgactac atcaaaatcg cgcgagagct
    17761 gttcgtgatg ctgcgtcagt cgccggaagg ccgcacactc ttttctcagc ttttacatct
    17821 aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta gaaacaccgg aagagtggcg
    17881 tgatgttcag aactcgcccg cattcgccgc acaaggctgg tttctttcac gcccggcacc
    17941 gatggaaacg ctgaatacgg cggttctggc gctataagct gcctcatttt ccgctgggct
    18001 ggactatctt taggactggc acaggaagaa tatgaggaag cgaacgatga gcaaggcagg
    18061 caaaataacc gctgcgattt caggggcttt cttgttgttg attgtcgtgg cgatcatttt
    18121 gattgcaact tttgactgga atcgactcaa accgaccatc aaccagaaag tctctgcgga
    18181 gttgaatcgt ccgttcgcta tccgtggcga tctgggcgtg gtgtgggagc ggcaaaaaca
    18241 agaaactggc tggcgcagct gggtgccgtg gcctcatgta catgcagaag acatcattct
    18301 tggtaatcca ccggatattc ccgaagtcac gatggtgcat ttgccgcgag tagaggcaac
    18361 gctggcaccg ctggcgctgc tgaccaaaac ggtctggctg ccgtggttca agctcgaaaa
    18421 gcccgacgcg cgcctgattc gcctctccga aaagaacaat aactggacgt ttaatcttgc
    18481 caacgatgac aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc ggctggataa
    18541 tattcttttc gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag cggatctgga
    18601 aatttttgtt gatccgttag gcaagccgct gccgttcagc gaagttactg gatcgaaagg
    18661 taaagcggat aaagaaaagg tgggcgatta cgtttttggc ctgaaggcgc agggacgtta
    18721 taacggtgaa ccgctcacgg gtacgggaaa aataggcggt atgctggcgc tgcgtggcga
    18781 agggacgccg tttccggtac aggctgattt ccgttcaggt aatacccgtg ttgcttttga
    18841 tggcgtcgtg aatgacccaa tgaagatggg cggtgtcgat ttacggctta aattttctgg
    18901 cgattcactg ggtgatctct atgaactgac gggcgttctg ctgcccgata ccccgccgtt
    18961 tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg tctttgatta
    19021 tcgcggtttt aatgggcgaa ttggcgatag cgatatccac ggttctctgg tctacaccac
    19081 cggaaagcca cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc ggctggcgga
    19141 cctgggaccg ttgattggcg ttgattccgg gaaaggggca gaaaagtcga aacggtctga
    19201 acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt atgaccgctt
    19261 cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc ggcgcattga
    19321 gcatggcagt agcctgccga ttagcgatct ttctactcat atcatcctca aaaatgctga
    19381 cctgcgcctg caaccgctga aatttggcat ggcgggcggc agcattgcga cgaatattca
    19441 tctggaaggc gataaaaagc cgatgcaggg gcgggcagat attcaggctc gtcgactgaa
    19501 actgaaagaa ctgatgcccg atgtggaact gatgcagaag acgctggggg aaatgaacgg
    19561 tgacgcggaa ctacgcggta gcggtaactc ggtggcggcg cttttaggca acagtaacgg
    19621 caacctgaaa ctgttgatga atgacgggct ggtgagccgc aacctgatgg agattgttgg
    19681 gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt gacgatgagg tgcgggtgaa
    19741 ctgcgcggcg gcgaatctga atattgccaa cggcgtggcg cgcccgcaga tttttgcttt
    19801 cgatactgag aacgcgttga ttaatgttac cggcacggca agttttgctt cagaacagct
    19861 ggatttgact attgatccgg agagtaaagg gattcggatt atcacactgc gttcgccgct
    19921 gtatgtgcgt gggacgttta aaaatccgca ggctggggtg aaagccggac cgctgattgc
    19981 ccgtggtgct gttgctgcgg cactggcaac gctggtaaca ccgacggcgg cgttactggc
    20041 actgatctca ccttccgaag gggaggctaa tcagtgtcgg acgatattgt cgcagatgaa
    20101 gaagtgatga taattgccgg atgtctgaca tccggcaatt aaacagattt agatttttgg
    20161 caaagtgtgc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaatcct
    20221 tgtgaattca atatattgta tggaaattca gtcctgataa gcgtagcgca tcaggctttt
    20281 cacacttatt acaacgactg atgtcgcgtc tcatgggtca gcagcagggc gattaacgtc
    20341 aagccagcca tcgccgccag atataacccc accgcaccta acccgtagtt agtctgcaac
    20401 caggctgcga tatacggtgc aacggaagcc ccgagaatcg acgctacgtt gtaagagaat
    20461 gatgctccgg tgtaacgcac ttctgttggg aacagctctg gtaacagcgc gcccatcggc
    20521 ccgaaggtca gccccatcag acttaacccc agcagcagga aggcaaaaac cagaatcggg
    20581 ttgccagaac cgagcagcgg gttaaaggcg aacagcgcga acaggatgat cagcgtggtg
    20641 ataattacca tgcttttacg gcgaccaaag gcatcagcca gtaatccggc gactggcacc
    20701 atcacgccaa aaccaataac tgccatcatc agcatccaca acacttcgtt acgcggcagg
    20761 ccaagcccaa ctggcgcggc ggcggtactg aaggtcatag agtagaccgt catgatgtaa
    20821 aacagcgtat aggttgccag catgatgaac gtacccagta cggttacgcg aacatgtttg
    20881 gtcagcagcg tacccagcgg gatcttcacc tgttttttcg ctttagcgac tttctcaaac
    20941 accggcgact catgcagcga cacgcgaaca tacaggccga taatgaccag taccgccgag
    21001 aagataaatg gcacacgcca gccccagctc ataaactgct cgtcggtcag tagccaggaa
    21061 agcagcaaga aagtgccgtt ggcaaagaag aagccaatcg gtgcgcccag ctgtgggaag
    21121 gagccataca gtgcacgttt gcgcggtggg gcgttttcag tcgccagcag cgccgcgccg
    21181 ccccattcac cgcctaagcc cagaccctga ccaaatcgag ccagcgccag cagcagcggg
    21241 gcgaaaatac caatcgtggc atagcccggc agcagaccaa tcaccacggt tgaaatcccc
    21301 atcgttagca acgaggcgac cagcgtcgct ttacgcccaa cgcgatcgcc aaaatgacca
    21361 aaaacggcag agccaatggg gcgcgcgacg aaggcgatgg cgaaggtggc gagcgactgt
    21421 agcgttgctg ccgttggatc gccctgcggg aagaagatat gcggaaacac aataacggcc
    21481 gcagtggcgt aaatgtaaaa gtcgaagaac tcaatggccg tgccaatgag agaggcgaca
    21541 aggactttat tacgcgagtt gatcggcgtg tattcttgct cgtggtcgag tgttgtggct
    21601 gttgcttgca taatcttttc ttattttttg gctaacgaat agccatatta cgcacagcaa
    21661 aagtgatatt tcaatgtgta acaaatcgcg gaaatggtca aaaaccaggc aaaaagggga
    21721 taatgtttag gccagtgatt tttcttctat gaaatgcttc acataatttt ataaatagtg
    21781 gataaaactg gtttcaggtt aaataaaagt taccaactgg atttatatgc tggggttatg
    21841 agttggaatg aaattgtttg ctcccaaata gatggctgaa gaggctctgg ggaagagttt
    21901 caagaggtta tgtgcgcatt atagccggat gcgatgctgg cgcatcttat ccggcctacg
    21961 gggagccagg ccgaataaaa tcgtatgccg gataaggcgt ttacgccgca tccggcctat
    22021 tttaaggctg cgttttccct ggcattcgcg ggtcgtcttt atattcggcg gtggcaatcc
    22081 acgccgcgca aaacagcgtc aaacgggcga agaagtagaa aaatgccatc agccccagca
    22141 cggaaccaaa tgctgcgcca gagggggatt tcatcagcga tggcagggtg taggtcatca
    22201 cgattttaat cacttcaaaa ccaatagcag ccagaaatgt tccgcgaatc agggcttttt
    22261 tgcgcggacg gtggcgcggt aaccgccaga agatccagaa gaaaagcaga tagttggcga
    22321 agatggaaat cgccaggcca atcaaccgcc acgtcggttt cagccactca atgctgttca
    22381 ggtgcagggc gctaataatc atttgctgcg ccgaaccggc aaccgaggtg atcgaaagcg
    22441 tcacaatcag cgcaatcaac aaaccaatca gcgaaataaa atcacgtaga tattttaccc
    22501 agaacttctc ctgatcttgc ggcgagcgtt cccagacatc gcgcgactgg gcgcgaatcg
    22561 cttcacgcag gttacccatc cagttgatgc cggaataaag cgccacagcc aggccgacaa
    22621 gccctacagt cgtacgctgc tgaacggcgg tgttgatggt gtttttcaac gtggcggcta
    22681 gcgtcggatc gctgatgttt tgcagaattt tgtcgaagat atcctgtagc aacatcggat
    22741 gggaggccag cacaaagccc cctgcggcaa acgacaccat caaaatcggg atcatcgaca
    22801 aaaatgagaa ataggtgata gccgcaccaa actggttacc cagccgatcg ttaaagcgtt
    22861 ctgtcgcgcg aatcaggtgc gcaataaccg gttggcgttg gactttttcg gcagtggtgg
    22921 tgacggtttc cagcgcctgg ctaactttgc cgcctttctc gctattatcc aactgcttaa
    22981 tcggctcgtg ctccagatcc tgggtgggac gtttgatctc gttttcctgc gtcatccgct
    23041 cttttgtcct tctgttgtgg tcattcttaa aaagtatagt cggtcagtcc aggtactctt
    23101 tcattacgcc agccagccac tgcataaaca ggtttacacg ccgggaaagc tcccgacgct
    23161 gcggataaac cagcgaaagg gagagcggct cggcacggta gcctggcaac acttcaataa
    23221 gctgcccggc acgcagggct tcgcgcacgg caatgcgcgg gatctgaata atccccagac
    23281 cggcaagacc ggcggcgaga tagttttcgc tactgtttac cgtcaacatg ccgccagact
    23341 taaaccactg gacgccatta acgctggcaa cctcaaaacc taacggatgt acacccaggt
    23401 gcggtgtata acgcaccatt gcgtgtgaag tcagatcatc ggggctttgc ggataaccaa
    23461 agcgcgtcag atagtgcgga ctggcacagt tgaccatggt cagtttgccg aggggacggg
    23521 cgataacgcc atcttccggt aacgcgccag tgcgtatcac gcaatcaaaa ccatcatgaa
    23581 gaatatctac cggacggtta tggctactca gttccagctc aattcccgga tgctgataga
    23641 gaaattccga cagacgcggc agtaacaggt ttttcgcgat tcctgacggg atgtcgatgc
    23701 gtaatttacc gctgatactg gtggcatcct gttgaaacag accgtccagt tcgctgagat
    23761 tactcaacac atcttttgcc cgttgataat aggtcatgcc ttctggcgtg agtttgaccc
    23821 gtcgcgtagt gcgctggaga agctgggtgc caagttgatg ttccagtgcc tgtatctggc
    23881 gcgaaacact tccctttggc aaagcaaaga aatccgccgc gcgggaaaaa ctttccagct
    23941 ccgcgacttt gatgaacaac tgcattgcgt gaattttatc catttctttg accgattgtt
    24001 gttcacagcg aaacagtaac gcgcatatcg cattgtttat tgttttttct aacatctaat
    24061 gccgggtttc acaatgttgt catgacgaat gtggcgattt ctcagcagga aaagggcgga
    24121 atagctttat gagaaaggtg aatcccctta aacagagcgt atttttcagt gattcccttt
    24181 aaagtgaata gaggacgtat tggctatttg tgcttatttt tgtctcattg ccatttgtta
    24241 ttttggcttg tagtcgcgga atttcatttt taattgcatg cgtactaacc accaatataa
    24301 ggaaatcctc aaaattgttt taaatagtgc cctcgacatg taatttcagg cgtttagctg
    24361 taactattta tatcggtaac tattttctac gcgtcatctc tgtagaatgg ccaccgccaa
    24421 atggcgaatt cttatttttt ctcgcgggta atacccttcc actacttaag tctttttaac
    24481 gtattcaggt ggagattcag ttatgcaaat agtcatgttt gacaggcagt caatatttat
    24541 tcatggaatg aaaatcagtt tacagcagcg tattccagga gtgagtattc agggggccag
    24601 tcaggcagac gagttatggc aaaagctgga aagttaccct gaagccttag ttatgctcga
    24661 tggcgatcag gatggtgagt tttgctactg gttgctgcaa aaaaccgtgg tgcagtttcc
    24721 tgaggttaag gtgttaatca cggcgacgga ttgcaataaa cggtggttac aggaagttat
    24781 tcattttaat gtgctggcca ttgtgcctcg tgattcaacc gtcgagacat ttgcgttggc
    24841 ggtaaatagt gcggcgatgg ggatgatgtt tctgcctgga gactggcgga cgacgccgga
    24901 aaaggacatt aaagatctca aatcgttaag cgcccgccag cgggagattt taaccatgtt
    24961 agcggcgggc gaatcaaata aggagatcgg cagagcgctg aatatcagta ccggaacggt
    25021 aaaagcgcat ctggaatccc tgtatcgccg tctggaagtg aaaaatcgca cccaggcggc
    25081 aatgatgtta aatatctcct cctg
//
LOCUS       DAJVRF010000053        24837 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJVRF010000053 DAJVRF010000000
VERSION     DAJVRF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.053"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(29..829)
                     /locus_tag="OQN13_001935"
                     /pseudo
     CDS             complement(29..829)
                     /locus_tag="OQN13_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            1396..1761
                     /locus_tag="OQN13_001936"
     CDS             1396..1761
                     /locus_tag="OQN13_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1615304.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            1801..2457
                     /locus_tag="OQN13_001937"
     CDS             1801..2457
                     /locus_tag="OQN13_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCS1615305.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            2524..3942
                     /gene="dcm"
                     /locus_tag="OQN13_001938"
     CDS             2524..3942
                     /gene="dcm"
                     /locus_tag="OQN13_001938"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCS1615306.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3923..4393
                     /gene="vsr"
                     /locus_tag="OQN13_001939"
     CDS             3923..4393
                     /gene="vsr"
                     /locus_tag="OQN13_001939"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCS1615307.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(4382..5303)
                     /gene="yedA"
                     /locus_tag="OQN13_001940"
                     /pseudo
     CDS             complement(4382..5303)
                     /gene="yedA"
                     /locus_tag="OQN13_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            5476..6393
                     /locus_tag="OQN13_001941"
     CDS             5476..6393
                     /locus_tag="OQN13_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCS1615308.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            6472..6654
                     /locus_tag="OQN13_001942"
     CDS             6472..6654
                     /locus_tag="OQN13_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCS1615309.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6825..8519
                     /gene="yedQ"
                     /locus_tag="OQN13_001943"
     CDS             6825..8519
                     /gene="yedQ"
                     /locus_tag="OQN13_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCS1615310.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(8516..9331)
                     /locus_tag="OQN13_001944"
     CDS             complement(8516..9331)
                     /locus_tag="OQN13_001944"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HCS1615311.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(9629..9856)
                     /gene="yodD"
                     /locus_tag="OQN13_001945"
     CDS             complement(9629..9856)
                     /gene="yodD"
                     /locus_tag="OQN13_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCS1615312.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            10019..10207
                     /gene="dsrB"
                     /locus_tag="OQN13_001946"
     CDS             10019..10207
                     /gene="dsrB"
                     /locus_tag="OQN13_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCS1615313.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(10251..10874)
                     /gene="rcsA"
                     /locus_tag="OQN13_001947"
     CDS             complement(10251..10874)
                     /gene="rcsA"
                     /locus_tag="OQN13_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCS1615314.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKKLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(11159..11944)
                     /gene="fliR"
                     /locus_tag="OQN13_001948"
     CDS             complement(11159..11944)
                     /gene="fliR"
                     /locus_tag="OQN13_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCS1615315.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(11953..12222)
                     /gene="fliQ"
                     /locus_tag="OQN13_001949"
     CDS             complement(11953..12222)
                     /gene="fliQ"
                     /locus_tag="OQN13_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCS1615316.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(12232..12969)
                     /gene="fliP"
                     /locus_tag="OQN13_001950"
     CDS             complement(12232..12969)
                     /gene="fliP"
                     /locus_tag="OQN13_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCS1615317.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(12969..13334)
                     /gene="fliO"
                     /locus_tag="OQN13_001951"
     CDS             complement(12969..13334)
                     /gene="fliO"
                     /locus_tag="OQN13_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCS1615318.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(13337..13750)
                     /gene="fliN"
                     /locus_tag="OQN13_001952"
     CDS             complement(13337..13750)
                     /gene="fliN"
                     /locus_tag="OQN13_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCS1615319.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLISQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(13747..14751)
                     /gene="fliM"
                     /locus_tag="OQN13_001953"
     CDS             complement(13747..14751)
                     /gene="fliM"
                     /locus_tag="OQN13_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCS1615320.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(14756..15220)
                     /gene="fliL"
                     /locus_tag="OQN13_001954"
     CDS             complement(14756..15220)
                     /gene="fliL"
                     /locus_tag="OQN13_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCS1615321.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            complement(15325..16452)
                     /gene="fliK"
                     /locus_tag="OQN13_001955"
     CDS             complement(15325..16452)
                     /gene="fliK"
                     /locus_tag="OQN13_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCS1615322.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(16449..16892)
                     /gene="fliJ"
                     /locus_tag="OQN13_001956"
     CDS             complement(16449..16892)
                     /gene="fliJ"
                     /locus_tag="OQN13_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCS1615323.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(16911..18284)
                     /gene="fliI"
                     /locus_tag="OQN13_001957"
     CDS             complement(16911..18284)
                     /gene="fliI"
                     /locus_tag="OQN13_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCS1615324.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(18284..18970)
                     /gene="fliH"
                     /locus_tag="OQN13_001958"
     CDS             complement(18284..18970)
                     /gene="fliH"
                     /locus_tag="OQN13_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCS1615325.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(18963..19958)
                     /gene="fliG"
                     /locus_tag="OQN13_001959"
     CDS             complement(18963..19958)
                     /gene="fliG"
                     /locus_tag="OQN13_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCS1615326.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(19951..21609)
                     /gene="fliF"
                     /locus_tag="OQN13_001960"
     CDS             complement(19951..21609)
                     /gene="fliF"
                     /locus_tag="OQN13_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCS1615327.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            21825..22139
                     /gene="fliE"
                     /locus_tag="OQN13_001961"
     CDS             21825..22139
                     /gene="fliE"
                     /locus_tag="OQN13_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCS1615328.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            22473..22805
                     /gene="emrE"
                     /locus_tag="OQN13_001962"
     CDS             22473..22805
                     /gene="emrE"
                     /locus_tag="OQN13_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCS1615329.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            22974..23525
                     /gene="ybcL"
                     /locus_tag="OQN13_001963"
     CDS             22974..23525
                     /gene="ybcL"
                     /locus_tag="OQN13_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCS1615330.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            23535..24332
                     /locus_tag="OQN13_001964"
     CDS             23535..24332
                     /locus_tag="OQN13_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1615331.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNSELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT         6112 a         6136 c         6351 g         6238 t
ORIGIN      
        1 actaaatcag taagttggca gcatcaccca ttgcaagata aacattgttg gcgtcgtatt
       61 ttgcaccaaa tgtccatgct tcagctgtat caccaccagc aaatttgctg tattcattgt
      121 agtgataccc gccaagagct acttgagaat tggtacggtc agagtttgag taagcggcac
      181 ctaagctcaa accgaagtca aagtcatatg aagtggaaag accaaaacca tcaccgttct
      241 ggaagcgaac gtcttcgtga ccatttttag taccttcttg atcttctcct gcattgctgc
      301 cttcgttgtt accttgatac tgcaatgcaa agttcagacc ttccaccaga ccgaagaaat
      361 cactattgcg gtaagtagct accccatttg cacggcctgc catgaagtta tcagtccagg
      421 tataggagtc gccgccgaat acaggaagca tatctgtcca ggcttcaacg tcgtaaatca
      481 caccatagtt acgaccatag tcaaaggagc ccatctcgcc gaacttcaga cctgcaaaag
      541 caagtttgac ataagtatta tcagaacctt cagtgtcgct agcctttgtt ttggtcagtg
      601 cttgaccata accagttaac tgatctgtaa tttgggtctc acctttgaag cctaactcga
      661 tgatagtttc atcgccgtca tcgccagggt tatcggaaaa agtatgacga gcatcaactt
      721 ttccgtacag atccagttta ttgccatttt tattataaat ttcagccgca tttgctgcgc
      781 cagcaactaa taacgctggg accagcattg ccagaacttt tcttttcatt atgtattccc
      841 ttgtgattat aatcttcatg aatatatcaa taagtgccgt tatccaaaaa aaaagcacat
      901 ttggatacta ttctatgaag ttcattttat tttaaagatt gcgtgtaaca aatgtattta
      961 aaagatttca aaatgtttct tatctatttg atataattca gaattatgaa ttctaaatat
     1021 aaacatccat gaaaattata aatgtatatc aatatgttat gagttgaatg gaaagatagg
     1081 gagtatcaat gattgttgcg ccaaacggca ggcgtgatca cgcgcacatt aatgaaggta
     1141 taaaaaaagc cggcatcatg ccggcccaaa aaatataaac aagacgataa aaggctaata
     1201 acggggttat catgacacag aaatcaaaaa aggtataatt gtattcagtg ctaatatatt
     1261 gtttgggtga attaataaaa ctattaccaa ggatataaat aaatctttat tgctgttttt
     1321 ccagtaatta ttgccgttgc gcaaaattta tctatttgtt caaaaaatga ttgagtcttg
     1381 actggctcat ccaatgtgga aaaatgtgac ttttatcaca taatagtact aagtctgaat
     1441 tttccgggtt atctcaaaat ggaatacggt tcgacaaaga tggaagagag actctctcgc
     1501 agccctggcg gcaaattggc tttgtgggct ttctatacat ggtgtggcta tttcgtctgg
     1561 gcaatggcgc gttacatctg ggtgatgagc cggatccctg atgccccggt gtcgggattt
     1621 gaaagcgatt taggctctac cgcgggaaag tggttagggg cactggtcgg atttttattt
     1681 atggctctgg tcggggcatt gttaggcagc attgcctggt acacccgacc tcgtcccgca
     1741 cgtagcagac gctatgaata aaaactgcta aggtggcgac tctttggcaa cggagcgcgt
     1801 atggacttac aacactggca ggcacagttc gaaaactggt tgaaaaacca tcatcagcac
     1861 caggacgcgg cacagaagct ggcggcagat gacgatgtcg atatgctggt gattttaacc
     1921 gcctgttatt ttcacgatat tgtgagcctg gcgaaaaatc atccgcaaag gcagcgttct
     1981 tcaatcctgg cggcagaaga gacgcgtcgc ctgctccgtg aagagttcga gcaatttccg
     2041 gcggagaaaa tcgaggccgt ttgtcatgcc attgccgctc acagtttcag cgcgcaaatc
     2101 gcccccttaa caacggaagc taaaatcgtc caggatgcgg atcggctgga agccctgggg
     2161 gcgattggcc tggcgcgcgt gtttgccgtt tcaggggcat tgggcgtggc gctgtttgat
     2221 ggtgaagatc cgttcgcaca gcatcgcccg cttgatgata aacgctacgc gctggatcat
     2281 ttccagacta agttgctgaa gctaccgcaa accatgcaaa ccgcaagagg caagcagctg
     2341 gcgcagcaca acgcgcattt tttagtcgag tttatggcga agctcagtgc cgaactggcg
     2401 ggggagaatg aaggtgtcga tcacaaggtg atagatgcgt tttcacccgc gggataagcg
     2461 cgtggcccta aatggctgta attatgttaa cctgtcggcc atctcagatg gccggttaaa
     2521 tctatgcagg aaaatatatc agtaaccgat tcatacagca ccgggaatgc cgcacaggca
     2581 atgctggaga aactgctgca aatttatgat gttaaaacgc tggtggcgca gcttaatggt
     2641 gtgggtgaga atcactggag cgcggcaatt ttaaaacgtg cgctggcgaa tgactcggca
     2701 tggcaccgtt taagtgagaa agagttcgcc catctgcaaa cgttgttacc caaaccatcg
     2761 gcacatcatc cgcattatgc gtttcgcttt atcgatctat ttgccggaat tggcggcatc
     2821 cgtcgcggtt ttgaatcgat tggcggacaa tgcgtgttta ccagcgaatg gaacaaacat
     2881 gcggtacgca cttataaagc caaccattat tgcgatccgg cgacgcatca ttttaatgaa
     2941 gatatccgcg acatcaccct cagccataaa gaaggcgtga gtgatgaggc ggcggcggaa
     3001 catattcgtc aacacattcc tgaacacgat gttttactgg ccggtttccc ttgtcagcca
     3061 ttttcgctgg ctggcgtatc gaaaaagaac tcgctcgggc gggcgcacgg ttttgcctgc
     3121 gatacccagg gcacgctgtt ttttgatgtg gtacgcatta tcgacgcgcg tcgtccggcg
     3181 atgtttgtgc tcgaaaacgt caaaaacctg aaaagtcacg accagggtaa aacgttccgc
     3241 atcatcatgc agacgctgga cgaactgggc tatgacgtgg ctgatgcaga agataacggg
     3301 ccggacgatc cgaaaatcat cgatggtaaa cattttctgc cgcagcaccg tgaacgcatc
     3361 gtgctggtgg gttttcgtcg cgatcttaat ctgaaagccg attttactct gcgtgatatc
     3421 agcgaatgtt tccctgcaca gcgagtgacg ctggcgcagc tgttggaccc gatggtcgag
     3481 gcgaaatata tcctgacgcc ggtgctgtgg aagtacctct atcgatatgc gaaaaaacat
     3541 caggcgcgcg gtaacggctt cggttatgga atggtttatc cgaacaatcc gcaaagcgtc
     3601 acgcgtacgc tgtctgcgcg ttattacaaa gatggcgcgg aaattttaat cgatcgcggc
     3661 tgggatatgg ccacgggtga gaaagatttt gacgatccgc tgaatcagca acatcgtcca
     3721 cgtcggttaa cgcctcggga atgcgcgcgc ttaatgggtt ttgaagcgcc gggagaagcg
     3781 aaattccgca ttccggtttc ggacactcag gcctatcgcc agttcggtaa ctcggtggtc
     3841 gtgccggtct ttgccgcggt ggcaaaactg cttgagccaa aaatcaaaca ggcggtggcg
     3901 ttgcgtcagc aagaggcaca acatggccga cgttcacgat aaggccactc gcagcaaaaa
     3961 tatgcgcgcg attgccacgc gtgatacggc gattgagaag cgcctcgcca gtctgttaac
     4021 cgggcagggc ctggcgtttc gcgttcagga cgccagtctg cccggaagcc cggattttgt
     4081 cgttgatgaa tatcgctgcg tgatatttac ccatggctgc ttctggcatc atcatcactg
     4141 ctatctgttt aaagtgcctg cgactcgaac cgagttctgg ctggagaaga taggtaaaaa
     4201 tgttgaacgc gatcgccgcg atatcagtcg tttgcaggaa ctcggctggc gcgtactgat
     4261 tgtctgggag tgtgcattac gtgggcgcga gaagctgacg gatgaagcgc ttaccgagcg
     4321 tctggaagag tggatctgcg gcgaaggtgc cagcgcgcag atcgacacgc aggggattca
     4381 tttactcgct tgatgcgtcc tgaataactg gcgcaactac gggttttgcc gggaagagat
     4441 attttcccaa cgtgaccagt accaccgcga agacaattac gccgagcgcc agccattcaa
     4501 tcttcgacag cgtctctccg cccagtcccg tacccagcaa gaccgcgacc accgggttaa
     4561 cgtaggcgta gctggtggcg agagccggac tgacattacg gattaaatac atataagcgt
     4621 tgatggcgat aatcgaacca aacagcgcca gatagccgac cgcaaggaag cctgaaaggg
     4681 aagggagcgc cgtcagtttt tcacctgcaa tcatcgacgc gatcattaac accacgcctg
     4741 ccgccagcat ctcaatcgca cccgccatca tccctacagg taaggtaatg cgcgagccat
     4801 aaaccgagcc aaacgcccag ctaatcgagc cgattaaaat cagaatcgcg ccccacggat
     4861 tgccgcttaa atttccaccg ctattgagca tgatgattcc ggcaagccca atggcgatac
     4921 ccacccattc cagtttgcgc gttttaatgc caaacaggcg gctgaagcac cagggtaaag
     4981 aggggcacgg ttgcaaccac aacggcggcg atgccggaag gaacattttg atgctcggca
     5041 accgtcacca tgccattacc gacagccagc aataacaggc caatcagcgc ggcattgagc
     5101 agcggacgta gcggggggag cttgtgtccg cgcagtagca aaaatgccat caataaaata
     5161 ccggctgcca ggaatcgaac gcctgccatc attaacggag gccagctttc cacgccaatc
     5221 cgaatgacaa aataggttga gccccaaatg atatacaacg caaacagcgc gccaaaaagc
     5281 ggtaacaact ggcggaaacg cataatccct cacggtggaa ataaaaaggt ggttcatagt
     5341 aaacgtgaaa atcattctgc tggcgagaga tataattgca cttgattgta aaaaaaatgt
     5401 tgacctgtga agcaagtatc agagctgccg tttttgcttc atacttacac ccttcaacaa
     5461 taaaaatgag agggaatgct tttggccggg agtagtttac tgacgttgct cgatgatatc
     5521 gccacactac tggacgatat ctccgtgatg ggcaaactgg cggcgaagaa aaccgccggt
     5581 gtattagggg atgacttatc gctcaatgcg caacaagttt caggcgtgcg ggccaaccgg
     5641 gaacttcccg tggtctgggg cgtggcgaaa ggatcgctga ttaataaagt gattctggtg
     5701 ccgctggcgc tgatcatcag tgcgtttatc ccgtgggcga ttacgcctct gttgatgatt
     5761 ggtggcgcgt ttctctgctt tgaaggagta gagaaagtgc tgcatatgct ggaggcgcgt
     5821 aaacataaag aagatccggc gcagagccag cagcgtctgg agaagctggc ggcgcaggat
     5881 ccgctgaagt ttgaaaagga caaaataaaa ggggcgattc gtaccgattt tatattgtct
     5941 gccgaaatcg tcgccatcac gctggggatt gtggccgaag cgccgttgct taatcaggtg
     6001 ctggtgcttt caggcatcgc gctggtagtg accgtgggcg tctacggtct ggtaggggtt
     6061 atcgttaaga ttgatgacct ggggtattgg ctggcggaaa aatccagcgc gctgatgcag
     6121 gcattaggta aagggttatt gatcattgcg ccctggttga tgaaggcgtt atcgattgtc
     6181 ggcacgctgg cgatgttcct cgtcggcggc ggtattgtgg tacatggtat tgcgccgctg
     6241 catcacgcca ttgaacattt cgccgggcag caaagtgcag tggtggcgat gatattaccg
     6301 actgttttaa atctgattct tggatttatc atcggcggca tcgtggtgct gggagtgaaa
     6361 gccgtagcga aaatgcgcgg tcaggcacat taatactcgt acaggctatt atgcaaaatt
     6421 cgccatcttc aactaaggtg aacaagtttc actcgaaaaa aggaggcgag tatgagcttt
     6481 atggttagtg aggaagttac ggttaaagag ggcggcccgc ggatgattgt cactggatac
     6541 tccagcggta tggttgagtg tcgttggtat gacggttacg gggtcaagcg ggaagctttt
     6601 catgaaaccg agcttgttcc gggggagggg agtcgttctg cggaagaagt ttgaaagggt
     6661 atcattaaca ccaggccgga tcacttccgg cctggtgtgc caatgcctgc aatgaatgat
     6721 agtcgcgtct tatcatgcca ccattataaa attatcgctg ttttgcggta cgctatcgcc
     6781 aggttatgcc agaatcataa aaaagcaggt tgggagtcgt cagggtgcag cacgagacaa
     6841 aaatggaaaa ccagagctgg ttgaaaaaac tcgcacgccg cctggggcct ggtcatgtcg
     6901 ttaatctctg ctttatcgtg gtattgcttt tttccacctt gctgacctgg cgtgaagtgg
     6961 tagtgctgga agatgcctat atctccagcc agcgtaatca tctggaaaac gtagccaacg
     7021 cgctcgataa gcatttgcag tataacgtcg acaaactgat ctttttgcgt aatggcatgc
     7081 gcgaagctct cgtagcgcca ctggatttca cttcactgcg taatgctgta accgagttcg
     7141 aacagcatcg cgacgagcac gcctggcaaa ttgaactcaa ccgacgacgc accctgtcag
     7201 tcaatggcgt atcggatgca ttagtcagcg aggggaatct cctgtctcgc gaaaatgaaa
     7261 gcctcgacaa tgaaattacc gctgcactgg aagttggtta cttgctgcga ctggcgcaca
     7321 acacctcgtc gatggttgaa caggcgatgt atgtctcgcg tgccggattt tacgtttcga
     7381 cgcagccgac cttgtttacg cgcaatgtac caacgcgtta ttacggctat gtcacccaac
     7441 cctggtttat cggccattcg caacgagaaa atcgtcaccg tgcggtacgc tggtttactt
     7501 cgcaaccgga acacgccagc aatactgaac cgcaggttac cgtcagtgtt ccggtagaca
     7561 gtaataacta ctggtatggc gtgctgggga tgagtattcc cgtgcgtacc atgcagcaat
     7621 ttttaagaaa cgccatcgat aaaaacctcg atggtgagta tcagctctat gacagtaagc
     7681 tgagattttt gacctcttcc aatcctgacc atccaacagg gaatattttt gatcctcgtg
     7741 aactggcctt gctggcgcag gcgatggaac atgacacgcg gggcggcatt cgtatggaca
     7801 gtcgctatgt tagctgggaa cgtctggacc atttcgacgg tgtgctggtg cgcgtccata
     7861 cgctaagcga aggcgtgcgc ggcgatttcg gcagtatcag cattgcatta accctgctgt
     7921 gggcgctctt taccaccatg ttactcatct cctggtatgt gattcgccgg atggttagca
     7981 acatgtatgt tctgcaaagc tcgttgcagt ggcaggcgtg gcacgacacc ttaacccgtt
     8041 tatataatcg tggcgcactg tttgaaaaag cccgtccgct cgcgaaattg tgtcagacgc
     8101 accaacatcc tttttctgtc attcaggtcg accttgacca ttttaaagcg attaatgacc
     8161 gctttggtca tcaggcgggc gaccgtgttc tttctcatgc tgccggatta attagcagtt
     8221 ccttgcgtgc gcaggacgtt gccgggcggg tcggtggtga ggagttttgt gtgattctgc
     8281 caggcgcgag tctgacggag gctgcggaag tcgcagaacg tattcgcctg aagttaaatg
     8341 aaaaagagat gttgattgct aagagtacga cgatacgcat cagtgcctcg ttgggggtaa
     8401 gtagcagcga ggaaaccggt gattatgatt ttgaacaact ccagtcactg gctgaccgtc
     8461 ggctttatct cgctaaacag gccgggcgta atcgggtatg cgcgagcgat aacgcttaac
     8521 gagcggagaa aaaatggtcc agcccttcac gccagccttc cggtccttca cgctgcgttc
     8581 gccagacgcg ggtcggatcc tcatcatgca gatgcacccc ttcacggttt agccctttca
     8641 caatcaccgc gtaatccatt acctccagta agggcgcatc gtttggccca tcgcccaggc
     8701 caagtgtggt tgggcgtttg cctgataatt gttgataggt cgcgataatc cagttggcgg
     8761 cctgatcttt tccggcagag gcatccagga cgtgccagaa gcgcgcaccc tgcataaact
     8821 gtaagcccag ttcgttcaga cgagcggtaa attgtgccat acgctcgtca ctgtcgcgcc
     8881 agattagcgt taccgacgcc tcatgcagct gcgtcagcgc cgcctggcta cggcttaatc
     8941 ccgtccattc ggcgatggtt gcatcgtcga catcatcgaa agtcgtgaat ttaaaatgtt
     9001 ctttctcgcg tagcgtattt aaaacctggc tgatttcgcc atggctaata cctgagatga
     9061 tgcgtggaaa accgtctata tcctgccatt gttcagcaag ctggatcact gcgccatttt
     9121 cggcaatcag tggtaaccct tgtagcccca acgttttttg caagtacagc atttccgctg
     9181 aagttttact gctacagaga atgacgggaa cattcgcttc gcgtaaacgg ctgagccaga
     9241 gggcagtcgg ttgccagtca taactatgac tgtccagcag ggtgccatca agatcgctaa
     9301 aaaccagtag tggttgttga attgaaaaca tggtcgtgca gttctcccct taccaggatg
     9361 attctcaaaa cagcacaaat aatcaaagat gattttttcg ggagcgatcc cgttgacgcc
     9421 agagccacca aggggtgacg cacgtcacat ttctattcat aagtagcgtt aattattcat
     9481 atcgcgaata ttttcttgtc agcgaaaaaa attgcggata aggtgatgaa cacatcagat
     9541 ttcctggtgt aacgaatttt ttaagtgctt cttgcttaag caagtttcat cccgaccccc
     9601 tcagggtcgg gattttttta ttgtgcattc aacgattcac ttcagagacg ctgaagtcat
     9661 gaatatccag ttcgaaggca tccgccaatt cacgccatgt atgatattca cgtccatcga
     9721 cgctaacgtg ttcagaatcg ttctggctgc gatgaacttc gctttcgcta atttcattga
     9781 tcgccgccag cagggcatcg acatcgacgc tgacctcacg ttttgcggta tcgctgtact
     9841 cttttgcggt tttcatcatc tttcctcgca accgttactc tgttgttgcc gccagtcgtt
     9901 aagtccgcct ggcatctctt aagtatagac cgtcgagaaa atcagcactc gcgcgcgcct
     9961 ggagctgcca gaaaaacgaa attgttctac actggcacaa agccacagga ggaaaacgat
    10021 gaaggtgaat gatcgggtaa cagtcaaaac ggatggcggt ccgcgtcgtc ctggcgtggt
    10081 actggcagtt gaggagttta gtgaaggcac aatgtacctg gtttcgctgg aagactaccc
    10141 gctcggcatc tggttcttta atgaagcagg gcatcaggac ggtatctttg tggagaaagc
    10201 agagtaatct tattcagcct gactggtggg aaaccaccag tcagaatgtg ttagcgcatg
    10261 ttgacaaaaa taccattagt cacattatcc gtcagtcgga cgacatggta gataacctgt
    10321 ttattatgtg ttttgatctt acgtttaata ttacctttat gcgatgaaac ggtcttggct
    10381 ttgatattca tttggtcaga gatttgaatg gttccctgac ctgccatcca cattcgcaac
    10441 atactcgatt cggttcggct caatgataac gtcggcatat ttaaaaaaga ggttatcgtt
    10501 gtctcttttt tcagaatatc gccaaggata tcgtcgagag attccggttt aatcgattta
    10561 gaacttatca ataatttttt tctgaccaat agatattcat caaaatgaac attggcaatt
    10621 gccataaaaa cgataaataa cgtattggga tgttgattaa tgatgtgctt gatgtgctga
    10681 ctgttagaag catcatggat gaaacagtcc tcattaataa acaccactga agggcgctgt
    10741 gaatcacaag ctatggcaag gtcatcaacg gtttcaatgt cgttgatttc tcttttttta
    10801 acccctctac tcaacagata cccggttaaa cctagtcggg tgtaactaca taaatccata
    10861 ataatcgttg acatggcata ccctcactca atgcgtaacg ataattcccc ttacctgaat
    10921 atttcatcat gactaaacgg aacaacatgg gtcacctaat gcgccactct cgcgattttt
    10981 caggcggact tactatcccg taaagtgttg tataatttgc ctggaattgt cttaaagtaa
    11041 atgttgcgat atgtgagtga gcttaaaaca aatatttcgc cgcaggagta tcctggaaga
    11101 tgttcgtagg tattattttt cggataatcc ttaggataac atgataaacg ttacggaatt
    11161 atattaatgg caattcacta ataatatcag ccagcaaatt aaacatctca ctgaataaat
    11221 gttcgcaaaa aggtgcaatt aacggcatta atgccgccat taaagagata ccgacagtca
    11281 gagttaatgg aaatccaata acaaaaatgg ataattgcgg ggccatacga ttcagtaaac
    11341 ctaatgccag attcagggtc agcagcagag taatgagcgg taacgccagc atcagcccgt
    11401 taaggaaaat caaactcccc gctttggtga gtgccagaaa cgcattgctg ttcaacggtt
    11461 cgccaccaat tggcagggtg tgaaaggtat cgaccagcag tgaaatcaac cataaatgac
    11521 cgttaaatgt caggaacagc agtaacgcca gcatatccat gatacgcgct aaaacgggca
    11581 tattgagatg gctggccgga tcgacaaacg tcgcaaatga cagccccatt tgcagaccga
    11641 taatttcgcc agcggttcgc acagcggcaa aggcaaattg catggtaaaa ccaagcgcaa
    11701 tgccgatcag gatctgctgc acggccagcc acagagcaaa gaacgaaaaa acaggaacat
    11761 cgttggcagg taatgatggg gcaatgacga acgtgatcat cattgccagc cccagtttta
    11821 cccgtttcgg cacgctgcgt tcgctcagaa ttggtgcggt agaaatgagc gccagcacgc
    11881 gcagtaacgg ccagaagtac aggcttaacc aggaaagcca ttgatcgctt gtcacctgca
    11941 tcattgtacg gcctacccga tgatatacgg caggttagtg aacaaggtgc ggacgtaatc
    12001 cagcaacaga ttgagcatcc acggtccggc aataataatg gcaataaata cggcgatgat
    12061 ttttggaata aacgacagcg tcatttcgtt gatctgcgtg gcggcctgca aaatactgat
    12121 gataaggccc gtgaccagcg ccaccagcaa cagcggggca gccagtgcca gcgcgacttt
    12181 catcgcttca gtccccatca tcatgaccga ttcaggtgtc attttgcctc tctagctgta
    12241 aaagctctgc gccagcgaac cgaccagcaa ttgccagcca tccaccagta caaacagcat
    12301 cagtttaaag ggcagagcaa tggtggcggg ggggaccatc atcatcccca atgccatcaa
    12361 cacgctggca atcaccaggt cgataatcaa aaaaggaatg aaaatcgtga agcctatctg
    12421 aaatgcggtt ttcaattcgc tggtcacgta ggccgggagc aaaatgcgca tcggcacggc
    12481 ttcaggtccc tgcaacgggc cggtattcgc tagtctggca aacaacccta aatctgcctc
    12541 acgggtctga cgcagcataa actcacgtag cggctgcgcc cctttttcca gcgcctcctg
    12601 cattgatatt ttctcttcgc tgaatggctg gtacgcatcg acataaattt tgtcgatcac
    12661 cgatgacata ataaaaaagg tcaaaaatag tgccagcccc agcaatacct ggttaggtgg
    12721 cgcggagggc gttcccagcg cgttacgcaa taaaccaaaa acaatgatga tgcgggtgaa
    12781 actggtcatc atcagtaaaa ttgccggaat aaacgtcaac gaggtgatga acaccagcgt
    12841 ctgcaccggg agcgaccagc tttgtccacc gccaggcagc ggctggctgg tgatacccgg
    12901 cagttgcgcg aaggcgaggg gcgtaaccag ccagagaagg acaggtgcga cagacaacaa
    12961 acgacgcatc aggatctccc actacgctta agcaaatttt tcatgaccga ctgaaaatcg
    13021 gtctgcggta tctcttctgt tggtgctgaa gggggaagtt tatgcagcag attgatttgc
    13081 cctgcggtaa cgccgagcac cagccgtgca tcttccacat cgaccaccac aacccgttca
    13141 cgcgcgccca gcgaagcact ggcgctaatt ttcagaccgt taacgccagt gcgtttcggg
    13201 gcaaatccca accgttttac cagccaggca gcagcgagga tcagggcaat aatggcgatg
    13261 agtgcgccgc tgacctgcag cagtggcgca gcagaaaccg gcgtggaaga ttgcacagta
    13321 gcgtggttat tcatcactaa cggctcaggc gacgcattcg ctcagacgga gtaatgatat
    13381 cggtgatccg cacgccatat ttatcggcaa cgaccaccac ttcgccctgg gagattaaat
    13441 aaccgttgat cagaatatcc agtggttcgc ccgccagacc gtccagcgcc acgacggacc
    13501 cttgcgtcag acgcaacagc tctttgatgg tcatccgcgt acggcccagc tcgacggtca
    13561 gcttgaccgg aatatccatt atcaggtcga tatcctgcaa cgttccgctg acatcaccac
    13621 cgccaaattg ctggaacacc gcgtcggcag cgcttttgct gctggttgat ttttgttcgc
    13681 tcaacgcttc agcccacaga tcgtccattg cgccgttgtt gtcatcggcc ggattattca
    13741 tgtcactcat ttgggctgtt cctcgttcag agaatttaaa atcgggttaa tcaaatgttc
    13801 tatccgtaac gcatactgac cgttgagagt gccgtactga ctggtcagca ccgggacgcc
    13861 gtcaacatgg gcgatgatgc catcgggttt ttctatcggc aggacgtcgc cgggtttcag
    13921 ttttaaaatc tgcgacaggc gtagcgagat atcggcaaag ttggcgacca gctccagctg
    13981 tgaatgctgc acctggcgca ccaggttatc gcgccagttc tgatcttcat tacgcgagtt
    14041 ttccagcggc gggttaacca acaattcccg tagcggctcg atcatgctga atggcagaca
    14101 gatattaaat tcgccggtca ggttgccaat ctccacatgg aacggcgtgt taaccacaat
    14161 gtcgttcggc gaggtggtga tattggtaaa tttcacctgc atttccgaac gcacgtactc
    14221 aacttccagc ggattaatcg ccttccaggc gtcgctatag ccttcaagcg ccagctttaa
    14281 catgcggttg atgacgcgct gttcggtatg ggtaaactcg cgaccttcca ctttggtcgg
    14341 gaagcgtcca ttgccgccaa acaggttatc cacggcgata aacaccagac tcggtgaaaa
    14401 caccaccagc cctgtgccgc gcagcggttt cagatggata aggttcaggt tggtcggcac
    14461 cggcaggttg cgggcaaatt catggtacgg ctgaatgcgg atggccccga cggttatatc
    14521 cgggctacga cgcagcaggt tgaacagccc catacgaaaa tggcgggcaa agcgctcatt
    14581 aatgatttcc agcgcctgca aacgttcgcg cacaacccgt cgttgggtat tcggatcgta
    14641 cggacgaatg tcactttcgc cgctaacact ggctgtcggt tcgtctttga cttcgctgtc
    14701 accattcaac agcgcatcaa tttcagcttg agaaagaata ctatcgccca tgtcgttatc
    14761 gcagaataaa agcggtatac atcacgtcgg tgacatcctg tttcggttgc ccggcaacaa
    14821 gcggggtgga aagcgtggtt ttaatctcgg caatcaggtt tttcttgcct tcttctgtcg
    14881 ccagtacggc agcatcctga cgcgaaaaca gtaacagcaa acggctacgg acttccggca
    14941 aatactcact cagccgcgag cgggtagctt catctttgag tcgcagggtt ataccgatat
    15001 aaagcacgcg atccgcatcg cccaaattga ccgtaaaggt atccagcgca tagaagaccg
    15061 gtgagggcac gacgcgatgc tgcgctttgt cttcagcggc aacctgatgc gaatgccagt
    15121 agctgtaacc tgcgctggca caggccgcga gggtaatgaa taccagaatc gggagccaaa
    15181 gcgatcgctt gcttttcttg cttatcgcgt aatcagtcat gtgttgcggt cttcctgtgt
    15241 cgctactgct tatcggctaa ttatcctgcg tcttgagcgc aacaaagcgg ggaaaagacg
    15301 cggattacgg tgctacctct gacgttaggc gaaaatatca acgccgctgt tgcctgttac
    15361 gcgcccttgt aaagagacgg gaaccggaag cgtatcgtct tcttcccccg ccagaggttc
    15421 atggtttact gtgcgttggc tttgctgttg ttgggaagcg gcctgctgct gaccactaaa
    15481 gctttcgcca ctgatgttgc tttgccctaa ctgaatgcca ctctcggcca gctgcgtacg
    15541 cagtaccggc agcgctgctt ccagggcggc gcgtacgtgc tgatgcggcg aaaccatctg
    15601 gatttgcgcc tggttatcat ccactttgag ggagatttgc acttcaccta aatcctgcgg
    15661 gtgcagacgc aactctgcac tttgttgtcc ctggcgggtg aacagcgaaa tatgctggct
    15721 taatgattgt tgccattcgt gagaacccag cggtgcactc aagacaggcg cggcgacggt
    15781 gagcagtggc tgtgtctggt gtggagtgat tagcgggctg gcggcagcgg tcaccggtga
    15841 aggtgtgctg atgacttccg ctttactctg ggcttctgct accagcggtg ttaatggctg
    15901 agctggcgtg ccgggggcat catcaggctg tgctgttgtg agttgcgcag aagtcagttt
    15961 tgtgaagagc gttggtttct ctgccggtaa cacggttgac ggcgcatcag tcactttggg
    16021 cgtattgtca aaacccggca acatcgcaaa aagggcgctc aggcttgcgg tgaggtcttc
    16081 attcagatca tccgcttttt cgtcttttgt cgtatttttg tcagccaccg cagccaacgt
    16141 catcgtatga gcggtggtta acggtgtcga tgtggattgt tcgacgttga tgacaggcag
    16201 tgtttcatcc acagggatca gtaaatccgc ttgttgcgcg tcggaaacaa tatcgctgat
    16261 cagcagctcg ccttttgtcg tgggcttatc tgttgccacc agcacctggg gggccgcttt
    16321 gtcggtagtt gtctcgcttg ctaatgcttc gctcaacaac gcgagaaaat cttgtgcagc
    16381 atcgctggct ttgccgccag gcaatgtggt ggtgtcaacg tcggcggtaa tcaagggcgc
    16441 taagcgaatc attcaggttt cctcatggcg gcgcgctggg cgaactcatc catctttttc
    16501 tgatcgaggc ggttttctgc aagcagtgcc gccgtggatt gccgttcctg cagtgtctgc
    16561 caggcctgca aacgttgttt cttttctcgc cagctgttca gggcaatgtc aactttctgc
    16621 gtccactgat taagttgctg gcgatgctga gtaatggctt tttccagcgt ctggataaac
    16681 tgctgatagt tgatccagcg gttgctggtc atcccggcac tcatatcgct gttgaggtta
    16741 ttgcgatatt cattctgata atcgatcagc attttgagct gttcttccgc ctgctgacac
    16801 ccgcgacgca tttcacccag caggcgcgcg gcatcctcta cctctttttc tgccagatct
    16861 ttcagggtcg ccagcgcacc atgttctgcc atctaccgtt atctcctggg ttatgacact
    16921 gtcgggaaaa tacgctccag cccctggaga gacgcttccc agtccgcacg ttcaaaaatg
    16981 ccttgttgta aatagccctc cagctgcggc cacagggcga tggctttatc gagcatcgga
    17041 tcgctgcctt tggcatacgc gccgacgcta accagatcgc ggttacgctg aaaactcgac
    17101 aacagctgtt tgaaggtgcg cactcgcgcg taatgttgct cactgatcaa cgccgtcatt
    17161 gcgcggctga tcgacgcttc aatatcgata gccggatagt gcccggcttc cgccagtcgg
    17221 cgagacagca caatgtgacc gtcgaggatc gcccgtgcgg agtctgcaat ggggtcctgc
    17281 tggtcatcgc cttcagtaag cacggtataa aacgcggtaa tcgagccgcc gccgctaatg
    17341 ccatttccgg cacgctcgac cagtgccggt aatttggcaa acaccgacgg tggataacct
    17401 ttggtggcgg gtggttcgcc aatcgccagc gcaatttcac gctgggccat cgcgtagcgg
    17461 gtgagggagt ccataatcag caacacatgc tgaccacgat cgcgaaaatc ttcggcaatg
    17521 cgcgtggcat aggcggcacc ttgcattcgc agaagcggag agacatccgc cggagcggca
    17581 atcaccacgg agcgtgcacg cccttcagca ccgaggatat tctcaataaa atcttttact
    17641 tcgcgcccac gttcaccaat caaacccacg acaatgacat cggcgcgggt gtaacgtgcc
    17701 atcatcccca gcagcacact tttaccaacg ccggacccgg caaacagccc catacgctgc
    17761 ccacgcccaa cggtaagcag ggcattgatt gggcgcacgc cggtgtccag cacatgttca
    17821 atcggtgtac gttgcaacgg gttaaatggc ggggtaatca gcgcaccggt ttccgtcgta
    17881 tcgggggagg gcaggccatc gagcggctta ccgctgccgt ccagaacgcg acctaataac
    17941 gccggaccga gcggcaactg cttgccgctt tgcagtcctt cagccgaaat gtttttggca
    18001 taaacccgcg cgccgggcag gacaccttcc acttcctcca gcggcattaa aaacagccgt
    18061 tgaccgttaa agccaacgac ttcgctttct acttcgtgcg tttcgctgcc gttctggcgt
    18121 tcaatgacac aggttgcgcc gagcggcaat tgtaatccgg tggcttccag caccagcccg
    18181 gtagcgcggg ttaatcgccc atagcgacgt accgcaggca actgcgccat tttggcttca
    18241 aagttatcca gcgtggttag ccagcgagtc aggcgcgtgg tcattacacc actcctggtg
    18301 ctgccagacg gcagagttct tgccagcgag tggcgacact ggcgtcgaga tcgccttcat
    18361 cggcggagac tttacagccg ccaggatgga gtgtgggatc gccccgcaag cgccagccat
    18421 gcaaacttaa ggttgcgccg agcatatcat ccacacgttg cagatcatcc gggtgcacgc
    18481 gcagctgtgg tttaccgctg aataacggtt cttgctgcaa caactgttgg atctgtttga
    18541 tcagtgccga gttatccact gttggcgtct gaccgatcac ctgacgtgcc gcctccagcg
    18601 ccatctgcat caggcgcgaa gcaatcacac tatcaagcgc atcaagggta gtttgaaatt
    18661 cgctgaccag ttgctgcatc cgggcatgaa ttggcgcttg ttgagacttc gcctctgcca
    18721 gaccttgctc cagcccctgg gccagtcctt cctgatagcc ctgctcatga ccttgctggc
    18781 gaccttcggc aatccccgcc tgataacctt gctcatgggc ctgcatttgc agttgcgcca
    18841 gttgctgctc aaggctaggt tcggcctctt caatgatggt ttcttccggc tcgaccatgg
    18901 gcacaaactc tgcctgtggt ggcgcgagat cgtccggcgt ccaggttttc cacggcagat
    18961 tatcagacat aggtatcctc gccgctgcca attaccatct cgccagtttc ggcaaggcgg
    19021 cgcacaatca gcagaatcgc tttctgttcg ttttccacct gcgacagacg caccggacca
    19081 cggttggcga gatcgtcgcg cagaatatcg gcggcacgct gcgacatatt gcgcaggaat
    19141 ttctcgcgca gtggctgctc ggcccctttc agcgcgatca acagcgattc ggaatccact
    19201 tcctgcaaca gacgctgaat gctgcgatcg tcgacatcca ccagattttc gaacaggaac
    19261 atctcgtcga taattttttg cgccagctcg ccgtcgaatt cgcgcacggc ggtaataacg
    19321 gcttcttcct gctgagtttt catcaggttg ataatttcgg ctgccgttct cacgccgccc
    19381 attttgctgc gcttgagatt ctgaccgtcg agcaagccat tcagtacttc ggtcagttcc
    19441 gccagcgcgg ctggctgcac gccgccaaag gtggcgatac gcaacatcac gtcgtggcgc
    19501 agacgttcat cgaacaacgc cagaatatcg gcggcctgcg cgcgcttcag atgcaccaga
    19561 atggtggcga taatttgcgg atgctcatcg cgaatcagat cggcggcact ctgcggctcc
    19621 ataaagttga gcgtttcaat accgctggcg gtatcgcgag tttcgagaat atcttccagc
    19681 aggctggcgg cacgttcctc acccagagct ttgaccaata ccgaacgcag ataatcgttg
    19741 gcgttgatat tcagtgcggc aaactgttca gcttcttgct caaactccgc cagcacatag
    19801 gttagctgct tgttggagat ctgcgtgacg ttcgccattg cagcgctcag ggtttgcact
    19861 tcgcgctggg agaggtgctt gaacacctct gccgcccggt cttcgccaat ggtcatcagc
    19921 aggatgacgc ttttatcggt gcctgtcagg ttactcatga tcgttattta tccactggcg
    19981 aatgaccagc gccaccacgc gcggatcgtt atcagacatt tcacggatac gctggctcat
    20041 gacttctgcc cccagacgtt ggttagcgcg tcgttgttgg agttgttcgt ctttgctcag
    20101 gcggacttcc accgcatctt ccacttcctc acgggcctgc gcctgttgct gtacagcttt
    20161 catcgcctcg gcgcgacgtg ttaactgcgg gcgtaccgct ttccgccaca gcagccacgc
    20221 caccagcagt accagcaacc agcgaccggc agccagcaac tgatcgataa acgcttgctg
    20281 ttgccagaac ggcagttctc cgccgctttc gtcactgcta ttgaacggcg agttaacgac
    20341 attgagcgag tcaccgcgtt tttcagaaaa gcccatcgct tcgcgggtca gatcttcaat
    20401 ttgcttcatc tgttcgttgc tgagaggcaa tggtttaccg tctggcaagg ttttgtaatt
    20461 caccactacc gcgactgaca gacgttgcac atcgcccacg ttcattttgg tatgacgaat
    20521 ggtgcgatcg acttcgtagt tactggtttc attccgctgt gtgctacgcg gcccgctatt
    20581 gctggtggtg ctcgcctgct gctggcggtt attttgattt gccggaggcg tgctgattgg
    20641 cgcgttattc gcaggtgccg gttgattcga caacgccccc ggtacgccgc ccggataacc
    20701 ggaaccgctt tgctcgctct cattaagctg gcgtgaacga agcgccgcat gagattcatc
    20761 accgttaggg cgatactgtt cttccgtttg ttctttactg gcgaagtcca gttgcgccgt
    20821 gacctgggcg tgaatattac cgttaccaac aataggcgac aggattgctt caatacgccg
    20881 ctgaatacgg ccttcgacat cgctggcata tttcaactga gcgtcattaa gatcgcgccc
    20941 gctggtattg gactgggtta acagatgtcc gccctgatcc accagcgtga cgtttcccgg
    21001 cggcagacca gcaacggcgc tggaaaccag gtgcacaatg gcgctaattt gcccttcatc
    21061 gagtgcgcgg ccgggtaaca gatttaccgt caccgatgca gaaggggatt tttgttcacg
    21121 gacgaataaa gacggtttcg gcattgccag atgtacgcgc gcccctttta ccgggccgat
    21181 agtttcgatg gtacgagaaa gctcgccttc cagcgcccgc tgataattca cctgttcgct
    21241 gaactggctg ataccaaact tttcctgatc aagcagttcg aaaccgaccg cgccgccttt
    21301 gggcaaacct tgttgtgcca ggcgcagacg cagttcgtga actttatctg ccggaacttc
    21361 aatagcgccg ctggcttcgc tgaagcagta aggaatattc atttgcgtca gttggctgac
    21421 aattgcgcca ccatcctgat cggaaagatt gctgaataat gtgcggtagt cgggggcttt
    21481 cgcccacagg atcagtgcga ccatgaccgc cactgccgcg gaaccggcaa caatcaatgg
    21541 aattttcgga ttcgcacgca ggcgattaag ccactcaaga gattttgtct gggctgcagt
    21601 cgcattcatc gcgcacctcg tggctgatta tggacggcgt tattggcaaa gtggaacaag
    21661 actcactccc gggtcagcaa actcgaaaaa ttgacgctgc cattatttgt gctttcgttt
    21721 ttttctatcc gccaataaac ccgttttttt tgttgctatt tagcgccttt gtcttattga
    21781 cttactggta agctttgcta ccagaaatta tccgggagat gaaaatgtca gcgatacagg
    21841 ggattgaagg ggttatcagc cagttacagg ctacggcgat gagtgcgcgt gcgcaggaat
    21901 cactgccaca accgaccatt agttttgccg ggcagctgca cgccgcgctc gatcgcataa
    21961 gcgatacaca aacagccgcc cgcacgcagg cagaaaaatt cactctcggt gaacccggcg
    22021 tggcgttaaa cgatgtgatg accgatatgc aaaaagcctc agtttctatg caaatgggga
    22081 ttcaggtgcg taataagctg gtggcggctt atcaggaagt gatgtccatg caggtgtagg
    22141 ttttgtaacc tgttgttaat tacatccgat ctcaccgcgt ggggcatgga tggggcaaac
    22201 tcactcaatt tctggtttaa aatggatacc tgatcctgaa agttttctgc catccacgat
    22261 ccgtaaacgc gataacatgt tttgtcttcg ctttgctcat aggaagagtg aactcttttg
    22321 ttagcctggg gttccttctg atcattattg ttcctgcctt tatctcttgc cacgccagag
    22381 ccccctggat acacatacac caggatgaaa acggtgagtt gtgttcaaac tgtagtacaa
    22441 ttctctccag tatgaacagg aaagaatatg ctatgaaccc ttatatttat cttggtggcg
    22501 caatacttgc agaggtcatt ggtacaacct taatgaagtt ttcagagggg tttacacggt
    22561 tatggccctc tgttggcaca attatttgct attgtgcatc attttggtta ttagctcaga
    22621 cactggctta tatacctaca gggattgctt atgctatctg gtcaggagtc ggtatcgttc
    22681 tgattagttt gctttcctgg ggattttttg gccaacgact ggacctgcca gccatcatag
    22741 gcatgatgtt gatttgtgcc ggtgtgttag tcattaactt gttgtcacga agcgcaccgc
    22801 attaaaaaat aatcatcctt gagataacag gaatctgatt tgtgcatatt atggctaatg
    22861 ttcggggcaa ctttataatt atgattgcat ttgcgcgatc attgcgcctt tttgcataat
    22921 gagatcaatg cacatcaaat aaaataacga taggtcacaa caaagaggtt tttatgaaaa
    22981 aacttatcgt ttcaagtgta ttggcattca taacattttc tgcgcaggcc gcagcatttc
    23041 aggtcactag taatgaaata aaaacaggag agcaacttac aacgtctcat gtattttctg
    23101 gagttgggtg tgaggggggt aatacatcgc cctcattaac ctggtctggt gctccagaag
    23161 gcaccaaaag ctttgccgta actgtgtatg atccagatgc gcctacaggc agcggatggt
    23221 ggcactggac tgttgctaat attccagcaa cggtaacata tttgcccgct gatgcaggaa
    23281 gacgtgatgg tacaaaactt ccgactggtg ctgtccaagg ccgaaatgat tttggctatg
    23341 cgggatttgg tggagcatgt cctcccaaag gagataaacc ccatcattac cagtttaaaa
    23401 tatgggctct aaaaactgat aagattcctg tggattctaa ttccagcgga gcgttagttg
    23461 gttatatgct taatgccaat aaaattgcaa ccgctgagat aatacccgtt tatgaggtaa
    23521 agtaggataa gagtatgctg gcaaaagata ggactaacct aaagattgaa gaaatacgga
    23581 tgcataaaca tcatgagatt cacagggtta agcctcttat gccagcttca tgtcgtatcc
    23641 gtcagggaaa aaaagttatc aattgggaga cgcatagtct aactgttgat aataatcaga
    23701 taatattatt tccctgtgga tatgaatttt atattgcgaa ttacccagaa gcaggtcttt
    23761 atcttgcaga aatgctttat tatcccattg atctaattga aaagttccaa aatctttatg
    23821 cgataaccga tcaaattcgt aacacgacag gtttctgctt acctcagaat tccgagttaa
    23881 tatattgttg ggagcaacta aaaacatcta tttcccgagg cttctcaacg caaattcagg
    23941 aacacttagc aatgggcgtt ctactttcat taggagcaca tcatgttaat tgtttacttt
    24001 tatcagatag taaacaatca ttaataagtc gttgttataa cctaatgcta tccgaacctg
    24061 gaacaaaatg gacagcaaac aaggtagcga gatatctcta catttctgtt tccacattgc
    24121 atcgccgtct ggcaagcgag ggagtaagtt ttcaaagtat attggacgac gtgaggttaa
    24181 ataatgcgtt gtctgctata caaacgacag taaaacccat cagcgagatt gccagggaaa
    24241 atggttacaa gtgtccttct cgttttactg aaaggttcca taatcgtttt aagataacac
    24301 caagagagct aagaaaagcg tccagagagt aaaagtattt tatgaaggag caacagcatc
    24361 gatttttatt ttggaaaata aatacggcat actcaaccta ttggagcctg aacaatccgg
    24421 tgacttctgc gctaatcggg gacgtttata agcacatata attgatacta tcttcctcca
    24481 tcacaatttc agaaatgcac gtgtgatttt tttgcatccg gcgtttggac tatgtggagt
    24541 tcaagagtgg gaaagttaca agaggttgag gggataatta aagggatgct tatgacatta
    24601 gatatccgga taggtacgga tatcgttgtt attaaatcta gttcgttatt attaaatcta
    24661 gtttatacgt taagatgcag ctacagcaga aattgccact gttaattttt tcatcgtgag
    24721 cccttttatt tgaactatta tttaaaaatg atgtcactgc actacaaata ttcatccaat
    24781 caatgagatt atttcaagat gtaagtttta gtttctcatt taaattgtga agtagat
//
LOCUS       DAJVRF010000054        23952 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJVRF010000054 DAJVRF010000000
VERSION     DAJVRF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23952
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.054"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(39..401)
                     /locus_tag="OQN13_001965"
     CDS             complement(39..401)
                     /locus_tag="OQN13_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCS1615332.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            938..2851
                     /gene="mtlA"
                     /locus_tag="OQN13_001966"
     CDS             938..2851
                     /gene="mtlA"
                     /locus_tag="OQN13_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCS1615333.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3081..4229
                     /gene="mtlD"
                     /locus_tag="OQN13_001967"
     CDS             3081..4229
                     /gene="mtlD"
                     /locus_tag="OQN13_001967"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS1615334.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4229..4816
                     /gene="mtlR"
                     /locus_tag="OQN13_001968"
     CDS             4229..4816
                     /gene="mtlR"
                     /locus_tag="OQN13_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCS1615335.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4828..5037)
                     /locus_tag="OQN13_001969"
     CDS             complement(4828..5037)
                     /locus_tag="OQN13_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1615336.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5322..5684
                     /locus_tag="OQN13_001970"
     CDS             5322..5684
                     /locus_tag="OQN13_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCS1615337.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            6227..6905
                     /locus_tag="OQN13_001971"
                     /pseudo
     CDS             6227..6905
                     /locus_tag="OQN13_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
     gene            6949..11799
                     /locus_tag="OQN13_001972"
     CDS             6949..11799
                     /locus_tag="OQN13_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HCS1615338.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            12167..13822
                     /gene="lldP"
                     /locus_tag="OQN13_001973"
     CDS             12167..13822
                     /gene="lldP"
                     /locus_tag="OQN13_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCS1615339.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            13822..14598
                     /gene="lldR"
                     /locus_tag="OQN13_001974"
     CDS             13822..14598
                     /gene="lldR"
                     /locus_tag="OQN13_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCS1615340.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            14595..15785
                     /gene="lldD"
                     /locus_tag="OQN13_001975"
     CDS             14595..15785
                     /gene="lldD"
                     /locus_tag="OQN13_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCS1615341.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            15981..16454
                     /gene="trmL"
                     /locus_tag="OQN13_001976"
     CDS             15981..16454
                     /gene="trmL"
                     /locus_tag="OQN13_001976"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCS1615342.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(16507..17328)
                     /gene="cysE"
                     /locus_tag="OQN13_001977"
     CDS             complement(16507..17328)
                     /gene="cysE"
                     /locus_tag="OQN13_001977"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCS1615343.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(17408..18427)
                     /gene="gpsA"
                     /locus_tag="OQN13_001978"
     CDS             complement(17408..18427)
                     /gene="gpsA"
                     /locus_tag="OQN13_001978"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCS1615344.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(18427..18894)
                     /gene="secB"
                     /locus_tag="OQN13_001979"
     CDS             complement(18427..18894)
                     /gene="secB"
                     /locus_tag="OQN13_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCS1615345.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(18957..19208)
                     /gene="grxC"
                     /locus_tag="OQN13_001980"
     CDS             complement(18957..19208)
                     /gene="grxC"
                     /locus_tag="OQN13_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCS1615346.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(19350..19781)
                     /locus_tag="OQN13_001981"
     CDS             complement(19350..19781)
                     /locus_tag="OQN13_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCS1615347.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            20026..21570
                     /gene="gpmM"
                     /locus_tag="OQN13_001982"
                     /gene_synonym="pgmI"
     CDS             20026..21570
                     /gene="gpmM"
                     /locus_tag="OQN13_001982"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCS1615348.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            21580..22863
                     /gene="envC"
                     /locus_tag="OQN13_001983"
     CDS             21580..22863
                     /gene="envC"
                     /locus_tag="OQN13_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCS1615349.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            22867..23826
                     /locus_tag="OQN13_001984"
     CDS             22867..23826
                     /locus_tag="OQN13_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCS1615350.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
BASE COUNT         6045 a         6108 c         6213 g         5586 t
ORIGIN      
        1 cagcaccacg taagttaaaa caatcaatag atccataatt agatctccgg gaacgtggaa
       61 tagtcagtct ttttctcggc ggcgagttga tgctctagcc gggaaattcg gtcagaaagt
      121 gcggcgattt ccgggtcagt cgctttctcc tgcgacgcaa catgtgactg catgccccac
      181 ccgcgctccg gttgatagag cgtcgcccag atccacagga acggccagat aacatgcagg
      241 gtaaacaggc tcacccagcc cgccgtatga atagcgtcgg catggggatg gttgcgcttt
      301 ttggcaatca gataagggat gtcatgtatc gcgatgattc cataaaaaat caccagaaag
      361 acgaagatca gcactcccaa cgcgaaatag tttagaaaca tatctgcctc ggattcacgt
      421 ttatcagtgt tgtttttggg ctggcagcca gaagggagtc aggctgatat tttgacaata
      481 atccgggttc gcgattctcg ccataacacc aaagaataat ttttagaggt gatgagttgc
      541 ttagttacat aacaattgta tgacgaaggc ataacatgct gtagatcaca tcaggtgaac
      601 gccgtaagaa aatatcttgt gattcagatc acaaagattc aacaaaccat caaaacaaaa
      661 atgtgacact actcacattt aaatgccatt tttagcgaaa atcgccgcct tgttgctttt
      721 ttacacaagc gttttgtgat gaacgtcacg ttaattacct ctctaccccc tatatttatg
      781 tgattgatat cacacaaaag gccgtcgact ggacagttaa ccgattcagt gccagatttc
      841 gcagtatcta caaggtccgg ctacctctgc cgccacatta acaaaaaacc tcgggcttcc
      901 agcctgcgcg acagcaaaca taagaagggg tgtttttatg tcatccgata ttaagatcaa
      961 agtgcaaagc tttggtcgtt tcctcagcaa catggtgatg ccaaatatcg gcgcgtttat
     1021 cgcgtggggt atcatcaccg cgttatttat tccaacaggg tggttaccga acgagacgct
     1081 ggcgaagctg gtcgggccga tgatcactta tctcctgccg ctgctgatcg gttataccgg
     1141 tggtaagctg gtaggcggcg aacgtggcgg cgtagtcggt gccatcacca ccatgggcgt
     1201 tatcgtcggc gcagacatgc cgatgttcct cggttctatg attgcaggtc cgctgggcgg
     1261 ctggtgcatt aagcacttcg accgctgggt agacggtaag atcaaatccg gttttgagat
     1321 gctggtgaat aacttctccg caggcatcat cgggatgatc ctcgctattc tggcattcct
     1381 cggcattggc ccgattgttg aagccctgtc caaaatgctg gctgcgggcg ttaacttcat
     1441 ggttgtccat gacatgctgc cgctggcgtc tatctttgtt gaaccggcga aaatcctgtt
     1501 cctcaacaac gccattaacc acggtatctt ctcgccgctg ggtattcagc agtcccatga
     1561 actgggtaaa tcaatcttct tcctgattga agctaaccca ggtccaggta tgggcgtgct
     1621 gctggcgtac atgttctttg gtcgtggtag cgctaaacag tctgcgggcg gtgcggcaat
     1681 catccacttc ctgggtggta tccacgaaat ctacttcccg tatgtgctga tgaatccgcg
     1741 tctgatcctc gcagtcatcc tcggcggtat gactggcgtg ttcacgctga ctatcctggg
     1801 cggtggtctg gtttctccgg catctccggg ttctatcctt gctgtactgg cgatgacacc
     1861 aaaaggtgct tatttcgcta acatcgcggg tgtgtgtgcg gcgatggctg tctccttcgt
     1921 tgtctctgct attttgctga aaaccagcaa agtgaaagaa gaagatgata ttgaagcagc
     1981 aactcgtcgt atgcaggaca tgaaagctga gtctaaaggc acatctccgc tgtctgcggg
     2041 cgatgtgact aacgacctga gccacgtacg taaaatcatc gttgcctgtg acgccggtat
     2101 gggttccagt gcgatgggcg caggcgtgct gcgtaagaaa attcaggatg caggtctgtc
     2161 acagatttcg gtcactaaca gcgcgatcaa caacctgccg ccagatgtgg acctcgtcat
     2221 cactcaccgt gacctgaccg aacgcgctat gcgccaggtt ccgcaggcgc agcatatttc
     2281 gctgaccaac ttcctcgaca gcggcctgta caccagccta accgaacgtc tggttgctgc
     2341 ccagcgccat actgaaaacg aagtgaaagt gaaagacagt ctgaaagaca gctttgacga
     2401 ttccagtgct aacctgttca aactgggcgc ggagaacatc ttcctcggtc gcaaagcagc
     2461 aaccaaagaa gaagcgattc gttttgctgg cgagcagctg gtgaaaggcg gttacgttga
     2521 gccggaatac gttcaggcga tgctggatcg tgaaaaactg accccgactt atctgggtga
     2581 gtctatcgcg gtgccacacg gtacggttga agcgaaagat cgcgtactga aaacgggcgt
     2641 ggtgttctgc cagtacccgg aaggcgtgcg cttcggtgaa gaagaagatg acattgcccg
     2701 tctggtgatt ggtattgcag cccgtaacaa cgagcacatt caggttatca ccagcctgac
     2761 caatgcactg gatgatgagt ccgtcatcga gcgtctggca cacaccacca gcgtggatga
     2821 agtgctggaa ctgttggcag gtcgtaagta atccaatccc accctctcca catggagaag
     2881 gtggggttaa ttgcctgatg cgctacgctt atcaggccta caggatgcat cacgatttgt
     2941 tgaatttgca cgttcttgta ggccggataa ggcgcttacg ccgcatccgg cgctgcccct
     3001 ctcctcacgg agagggtttg ggtgagggaa aagcctcacc ccagccctct cgggtaaaaa
     3061 cattgatgaa ggttaatact atgaaagcat tacattttgg cgcaggtaat atcggtcgtg
     3121 gctttatcgg taaactgctg gcagacgcgg gtatccaact gacgtttgcc gatgtcaatc
     3181 aggtggtact tgatgccctg aatgcccgtc atagctatca ggttcatgtg gttggtgaaa
     3241 ccgagcaggt agataccgtt tccggcgtca atgctgtcag cagcattggt gatgatgtcg
     3301 ttgatctgat tgctcaggtc gatttagtca ctaccgccgt tggcccggtt gtgctggaac
     3361 gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa agaacaaggt aatgaatccc
     3421 cgctgaacat catcgcctgt gaaaacatgg tacgcggtac cacgcagctg aaaggccatg
     3481 tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga agaacacgtt ggctttgtcg
     3541 attccgccgt tgaccgcatc gtaccgcctt cggcttcggc aactaacgat ccgctggaag
     3601 tgacggtaga aactttcagc gaatggattg tcgataaaac gcagttcaaa ggcgcactgc
     3661 cgaacatccc aggcatggag ttaaccgaca acctgatggc atttgtcgaa cgtaaactct
     3721 tcaccctgaa cacgggtcat gctataaccg cgtacctcgg aaaactggcc ggtcatcaga
     3781 ccattcgtga cgctattctc gacgagaaaa tccgcgcggt ggtaaaaggt gcgatggaag
     3841 aaagtggtgc ggtactgatc aagcgctacg gctttgacgc agacaaacat gcggcgtaca
     3901 tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa agatgatgta gagcgcgtag
     3961 gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct gatcaagcca ttgctcggta
     4021 cgctggaata cagccttccg cacaagaatc tgattcaggg gattgctggt gcaatgcact
     4081 tccgcagtga agatgatccg caggctcagg aactggcagc actgatcgct gacaaaggtc
     4141 cgcaggcggc gctggcacag atttccgatc ttgatgccaa cagcgaggtt gtatccgagg
     4201 cggtaaccgc ttataaagca atgcaataat ggtggaccag gcgcaggaca ccctgcgccc
     4261 gaataacaga ttgtcagata tgcaggcaac aatggaacaa acccaggcct ttgaaaaccg
     4321 tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc tttctgatca ccgccgtcga
     4381 gcttttgacc gaggcggtaa atcttctggt gcttcaggta ttccgcaaag acgattacgc
     4441 ggtgaagtat gctgtagaac cgttactcga cggcgacggt ccgctgggcg atctttctgt
     4501 gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc caggaatacg aagatgcgga
     4561 gctgctgatg gcattgcgtg aagaactaaa tcacgacggc aacgagtacg cctttaccga
     4621 cgacgagatc cttggaccct ttggtgaact gcactgcgtg gcggcgttac caccgccgcc
     4681 acagtttgaa ccagcagact ccagtttgta tgcaatgcaa attcagcgct atcaacaggc
     4741 tgtgcgatca acaatggtcc tttcactgac tgagctgatt tccagaatca gcttaaaaaa
     4801 agcctttcaa aagtaagcaa cgtctgctta ctgcccctct acctgcttcg gccgataaag
     4861 ccgacgataa tactccagac gttgtagata taacggcacg ctttcatcgg gtattccgga
     4921 cggaatcgcg ttacggggag gaagtttttt cagatactcc cggaacgcct ggcttgatgc
     4981 catgaaatct acggctttat cgataagaag cggaacgttt tcacctaatt tgcccatgat
     5041 gatatctccg tattaccccg cgccgggaat gcgcggccgc caattttagt ttaggttccc
     5101 ggtaaagatg acattaagga aaacgtgctg aatcctcaat aatcatccgc tgctgtttat
     5161 tatctttttt ctatagattt cttaattaat caataaattg cgtagttttt aagcatgtta
     5221 atgattacgt tacagaacta ttgcaatgtc atcagttgtg aacaaagcac ctggtcgcgc
     5281 atactagggg ctataaattt atctttatca gaagccatca catgaaagaa gtcgaaaaaa
     5341 acgaaatcaa acgtctcagc gatcgtctgg acgccatccg ccaccagcag gccgatctgt
     5401 cgctggttga agccgcagac aaatatgccg agctggaaaa agagaaagcc acgctggaag
     5461 cagaaattgc tcgcctgcgt gaagttcata gccaaaaact gagtaaagaa gcacagaagc
     5521 tgatgaagat gccgtttcag cgcgcaatta ccaaaaaaga gcaggctgat atgggcaagc
     5581 tgaagaaaag tgttcgcgga ctggtggttg tgcacccaat gaccgcgctg ggccgtgaaa
     5641 tgggcctgca ggagatgact ggattctcta agactgcgtt ttaagcgcct cagacgactg
     5701 gttcgatcat atcaacccgc atctggcttg ccttagctgg tcaatgcggg gactctcctg
     5761 tctactcgcc taattaccaa acaattcact ccctttacgt cgatttaccg ctggttgaga
     5821 ttctgaaaat gcatttttac cgaaatagcc acctataaac aataaaaata tatagaagat
     5881 atcatttatt aattaacaag cagttacgtc tctttttttc ggatttaatt gtgaaatttt
     5941 aatcatcata ttcatacaat aaattatctg taaatttttt cccagacatg gtttctttca
     6001 tatttgacat ttattaataa tcatcattag aatccacgaa aataatatat tcatacaaac
     6061 acagacatct tttgaaacga atgatacatt tttatgatga atgcttaaaa caatatttaa
     6121 tcgacacaca tcactgtcat taatgtaaag caacatagat caccaataat acaatggaca
     6181 actctaccgg cattgatcgc tggtcggagg aaataaggat ttttacatga acttgaagaa
     6241 gatttttttc agtgctgtca ctgtttcagt tctctgcgca ttaacgggat gtgactacat
     6301 tgaagaaggc aagccagaaa gtagtttatt gaaacaacaa gaagaacatt aaaattgatc
     6361 tacttgaaaa acaacaagca cagctaaaga gtcaactgga aactattcaa aaacaacaaa
     6421 ctggcataat aagcagtaca aagactttaa ctcacgtaat taaatccgtt aaagatcaac
     6481 aaaacacttt tatttttacc gagtttaacc cagcaaaaac gaagtatttc atcctgaaca
     6541 atggttcggt ggctttagcg ggtcgagtgt tatccattga cgccacagag aatggcagtg
     6601 ttattcatat ttcactggtc aacttattaa gtacacctat ctcaaatatt ggttttaatg
     6661 cgacatgggg tggtgaaaaa cctgtcgatg ccaaagagtt tgcccggtgg caacaattac
     6721 ttttcaacac atcaatgaaa tccacattga aattattacc aggtcaatgg caagacatta
     6781 atttgaccct gaagggtgta tcgcccaata acctgggata tctgaagtta gccatcaaca
     6841 tggaaaatat tcagttcgac aatcttccct ctgctgaaaa tcggcagaaa agaagcaaaa
     6901 aataaatgca gttagtacgc ttataacaag gaatagcgag catcattaat gaacaaaata
     6961 tttaaagtta tctggaaccc tgcgacaggg aattatactg ttaccagcga aacggcaaaa
     7021 agccgtggca agaaatctgg gcgcagtaag ctgttaattt ctgcgctggt tgcgggtgga
     7081 atgttgtcgt cgtttggggc attggcgaat gccgggaatg acaacggcca gggtgttgat
     7141 tacggtagtg gatcagctgg cgacagctgg gttgctatag gcaaaggggc gaaagcaaat
     7201 acttttatga acaccagtgg ttccagtact gctgtgggtt atgacgctat agctgaaggc
     7261 caatatagct ctgccatcgg gtcaaaaacc catgcgattg gtggtgcatc aatggccttt
     7321 ggggttagtg caatatcaga aggcgataga agtatagcac tgggtgcctc ttcgtattca
     7381 ttgggccaat actcaatggc cctcggccgt tattcaaaag cattgggtaa attgtctatt
     7441 gctatggggg actcttccaa agcggaagga gcaaacgcca ttgccctggg aaatgccact
     7501 aaagctactg agattatgag tattgctctt ggcgacaccg ccaatgcgtc aaaagcgtat
     7561 tcaatggcgc tgggagcaag tagcgtcgca tctgaagaaa acgcaattgc cctggggcgt
     7621 agcagtgtag ctagcggtac tgacagcctc gcatttggca gacaatcact tgccagcgca
     7681 gcgaacgcta ttgcgatagg tgctgagacc gaagccgctg aaaatgcaac tgctattggc
     7741 aataatgcga aggcaaaagg gactaatagc atggcaatgg ggttcggaag ccttgccgat
     7801 aaagtcaata ctatcgcatt aggaaatggc agccaggctc tggcagataa tgcaatcgcc
     7861 ataggccagg gcaacaaagc tgatggcgtg gatgccatcg ctctgggtaa tggtagccag
     7921 tcgagaggct taaacaccat tgccttaggc acagccagta atgcaactgg tgataagagt
     7981 cttgcgcttg gtagtaatag cagtgccaac ggtattaact ctgtcgcgct gggcgcagat
     8041 tccattgcgg atttagacaa taccgtctct gtcggcaata gttcattaaa acgcaagatc
     8101 gttaatgtga aaaatggcgc gatcaagtct gacagttacg atgccattaa tggttcacag
     8161 ctttatgcca ttagcgactc ggtagcaaaa aggcttggag gaggggctgc agtagatgtt
     8221 gatgacggta ctgttacagc accaacctac aatttaaaaa atggtagcaa aaataacgta
     8281 ggggctgcgc tcgctgtact tgatgaaaac accctgcaat gggaccaaac caaaggcaaa
     8341 tacagcgctg ctcatggtac tagtagccca actgccagcg taatcaccga tgttgcggat
     8401 ggcacgattt cagcctccag taaggatgcg gttaacggtt cccaactgaa agctaccaat
     8461 gacgatgtcg aagccaacac cgccaatatc gctactaata ccagcaacat tgccacgaat
     8521 acagcaagta ttgccaccaa taccaccaat atcaccaacc tgacggattc cgttggtgac
     8581 cttcaggctg atgccctgct ctggaacgaa actaaaaagg cattcagtgc agctcacggc
     8641 caggatacca ccagcaaaat caccaacgtt aaagatgccg acctgacggc tgacagcact
     8701 gatgctgtta acggctctca gctgaaaacc accaacgatg ctgtggcgac gaataccacc
     8761 aatatcgcca ataacacttc caatattgcc actaacacca ccaacatctc taatctgact
     8821 gagacggtga ctaatcttgg tgaggatgcg ctgaaatggg ataaggacaa tggtgtattc
     8881 acggcagctc atggcaccga gaccaccagc aaaatcacca acgttaaaga tggcgacctg
     8941 acgactggca gcaccgatgc cgttaacggc tctcagctga aaaccaccaa cgatgccgtg
     9001 gcgacgaata ccaccaatat cgccactaac accaccaaca tctctaatct gactgagacg
     9061 gtgactaatc ttggtgagga tgcgctgaaa tgggataagg acaatggtgt cttcactgca
     9121 gctcatggca acaataccgc cagcaaaatc accaatatcc tggacggcac agtcactgca
     9181 accagttccg atgccattaa cggtagccag ctttatgact taagcagcaa tatcgccacc
     9241 tacttcggcg gcaatgcttc tgtgaatact gacggtgtgt ttaccggtcc aacctacaaa
     9301 atcggtgaaa caaattatta taacgtcggc gatgcactgg ctgcgattaa ctcctcattt
     9361 agcacgtctc tcggcgatgc tctgctttgg gatgccaccg caggtaaatt cagtgccaaa
     9421 cacggtacta atggtgacgc aagcgtgatc actgatgtcg cagatggtga aatttcagac
     9481 tccagttctg acgcagtaaa cggctcacaa ctccacggcg tgagcagtta tgttgttgat
     9541 gcgctggggg gtggtgccga agtcaatgca gacggcacca tcactgcgcc gacgtacacc
     9601 attgctaatg ctgattacga taatgtcggt gatgccctga atgctatcga taccactctt
     9661 gacgacgctc tgctctggga tgcggacgcc ggtgaaaatg gtgcatttag cgccgctcac
     9721 ggaaaagata aaactgccag tgtaatcact aacgtcgcta acggtgcaat ctctgctgcc
     9781 agcagcgacg cgattaacgg ctcacaactc tataccacca ataagtacat cgctgatgcg
     9841 ctgggtggtg acgcagaagt caacgctgac ggcaccatca ccgcaccgac ttacaccatt
     9901 gcgaacgccg agtacaacaa cgtcggtgac gccctggatg cgcttgatga taacgccctg
     9961 ctgtgggatg agactgccaa tggcggtgct ggagcctaca atgccagcca tgacggtaaa
    10021 gccagcatca tcactaatgt cgctaatggc agtattagtg aggacagtac cgatgcagtg
    10081 aacggttctc agttgaatgc gacgaatatg atgattgagc agaacaccca aattatcaat
    10141 cagctcgctg gtaacaccga cgcaacctat atcgaagaaa atggcgctgg tatcaactac
    10201 gttcgtacta acgacaacga tttagccttt aacgatgcaa gcgcctctgg tgtcggcgct
    10261 acagctgtag gttataacgc tgtcgcgtct ggtgccagca gcgtagccat tggtcagaac
    10321 agcagcagca ccgttgatac cggtattgcg ctgggtagca gctccgtttc cagccgtgtg
    10381 attgccaaag gttctcgtga cactagcgta acggaaaatg gcgtggtgat tggttatgac
    10441 accactgacg gcgaactgct aggtgcattg tcaattggtg atgacggtaa ataccgccaa
    10501 atcatcaacg tagctgatgg ttcagaagcc catgacgccg ttacggttcg ccagttgcag
    10561 aacgctattg gagcggtcgc cactacgcca accaagtact ttcacgccaa ctcaacggca
    10621 gaagactcac tggccgttgg tgaagactcg ctggcaatgg gtgcgaaaac tgtcgttaat
    10681 ggtaatgcag gtattggtat cggtttgaac actctggttc tagctgatgc gatcaacggc
    10741 attgctatcg gcagtaacgc acgcgcaaac catgcaaaca gtatcgcaat gggtaacggt
    10801 tctcagacca cccgtggtgc acagactggc tacaccgcct acaacatgga cgcaccgcag
    10861 aactctgtag gtgagttttc tgtcggtagt gaagacggtc aacgtcagat cacaaacgtc
    10921 gcagctggtt cagcggatac cgatgcggtt aacgtgggtc agttgaaagt cactgatgag
    10981 cgcgtagcgc aaaataccca gagcattact aacctgaaca atcaggtcac taatctggat
    11041 actcgcgtta ctaatatcga aaacggtatt ggcgacattg tcaccaccgg tagcaccaag
    11101 tacttcaaga ccaacaccga tggcgtagat gccaacgccc agggtaaaga tagcgttgct
    11161 attggttctg gttccattgc tgccgctgac aacagcgtcg cactgggtac cggttccgtt
    11221 gcagaggaag aaaatacaat ctctgtaggt tcttctacta accaacgccg tatcaccaac
    11281 gtagctgccg gtgttaatgc caccgatgcg gttaacgtgt cgcagctgaa atcttctgaa
    11341 gcaggcggcg tgcgctacga caccaaagct gatggttcta tcgactatag caatatcacc
    11401 ctcggtggtg gcaacggcag tacgactcgt atcagcaacg tctccgctgg cgtcaacaac
    11461 aacgacgcgg tgaactacgc gcagttgaag caaagcgtgc aggaaacgaa gcaatacacc
    11521 gatcagcgga tggttgagat ggataacaaa ctgtctaaaa ctgaaagcaa gttgagcggt
    11581 ggtatcgctt ctgcaatggc aatgaccggt ctgccgcagg cttatacacc gggtgccagc
    11641 atggcttcta ttggtggcgg tacttacaac ggtgaatcgg cagttgcttt aggtgtatcg
    11701 atggtgagcg ccaatggtcg ttgggtctac aaattacaag gtagtaccaa tagccagggt
    11761 gaatactccg ccgcactcgg tgccggtatt cagtggtaat gatccattaa gttagtgtga
    11821 ctaagggcaa acgccaaatt gcctgatgcg ttacgcgcat caggcaagat aaggcgcccc
    11881 gggtcaacaa tctaagggca attctctgat gaggattgcc cttttcttta ccagacatct
    11941 ccccccacaa gaattggccc taccaattct tcgcttatct gacctctggt tcacaattcc
    12001 ccaattaaaa ctcacatcaa tattgccaat acataacatt tagttaacca ttcattgtca
    12061 ttatccctac acaacacaat tggcagtgcc acttttacac aacgtgtgac aaggagatga
    12121 gcaacagact cattacacga tgtgcgtgga ctccaggaga cctgcaatga atctctggca
    12181 acaaaactac gatcccgccg ggaatatctg gctttccagt ctgatagcat cgcttcccat
    12241 cctgtttttc ttctttgcgc tgattaagct caaactgaaa ggatacgtcg ccgcctcatg
    12301 gacggtagca atcgcccttg ccgtggcttt gctgttctat aaaatgccgg tcgctaacgc
    12361 gctggcctcg gtggtttatg gcttcttcta tggtttgtgg cccatcgcgt ggatcattat
    12421 tgcagcagtg ttcgtctata agatctcggt gaaaaccggg cagtttgaca tcattcgctc
    12481 gtctattctt tcgataaccc ctgaccagcg tctgcaaatg ctgatcgtcg gtttctgttt
    12541 cggcgctttc cttgaaggag ccgcaggctt tggcgcaccg gtagcaatta ccgccgcatt
    12601 gctggtcggc ctgggtttta aaccgctgta cgccgccgga ctgtgcctga ttgttaacac
    12661 cgcgccagtg gcatttggcg cgatgggcat tccaatcctg gttgccggac aagtaacagg
    12721 catcgacagc tttgagattg gccagatggt ggggcggcag ctaccgttta tgaccattat
    12781 cgtgctgttc tggatcatgg cgattatgga cggctggcgc ggtatcaagg agacgtggcc
    12841 tgcggtcgtg gttgcgggcg gctcgtttgc catcgctcag taccttagct ctaacttcat
    12901 tgggccggag ctgccggaca ttatctcttc gctggtatca ctgctctgcc tgacgctgtt
    12961 cctcaaacgc tggcagccag tgcgcgtatt ccgttttggt gatttggggg cgtcaaagat
    13021 tgatatgacg ctggcccaca ccggttacac tgcgggtcag gtgttacgtg cctggacacc
    13081 gttcctgttc ctgacagcta ccgtaacact gtggagcatc ccgccgttta aagccctgtt
    13141 cgcatcgggt ggtgcgctgt atgagtgggt gatcaacatt ccggtgccgt acctcgataa
    13201 actggttgcc cgtatgccac cagtggtcag cgaggctaca gcctatgccg ccgtgtttaa
    13261 gtttgactgg ttctctgcca ccggcaccgc cattctgttt gctgcactgc tctcgattgt
    13321 ctggctgaag atgaaaccgt ctgacgctat cagcaccttc ggcagcacgc tgaaagaact
    13381 ggctctaccc atctactcca tcggcatggt gctggcgttc gcctttatct cgaactattc
    13441 cggactgtca tcaacactgg cactggcact ggcgcacacc ggtcatgcat tcaccttctt
    13501 ctcgccgttc ctcggctggc tgggggtatt cctgaccggg tcggatacct catctaacgc
    13561 cctgttcgcc gcgctgcaag ccaccgcagc acaacaaatt ggcgtctctg atctgttgct
    13621 ggttgccgcc aataccaccg gtggcgtcac cggtaagatg atctccccgc aatctatcgc
    13681 tatcgcctgt gcggcggtag gcctggtggg caaagagtct gatttgttcc gctttactgt
    13741 caaacacagc ctgatcttca cctgtatggt gggcgtgatc accacgcttc aggcttatgt
    13801 cttaacgtgg atgattcctt aatgattgtt ttacccagac gcctgtcaga cgaggttgcc
    13861 gatcgtgtgc gggcgctgat tgatgaaaaa aacctggaag cgggcatgaa gttgcccgct
    13921 gagcgccaac tggcgatgca actcggcgta tcacgtaatt cactgcgcga ggcgctggca
    13981 aaactggtga gcgaaggcgt gctgctcagt cgacgcggcg gcgggacgtt tattcgctgg
    14041 cgtcatgaca catggtcgga gcaaaacatc gtccagccgc taaaaacgct gatggccgat
    14101 gatccggatt acagtttcga tattctggaa gcccgctacg ccattgaagc cagcaccgca
    14161 tggcatgcgg caatgcgcgc cacacctggc gacaaagaaa agattcagct ttgctttgaa
    14221 gcaacgctaa gtgaagaccc ggatatcgcc tcacaagcgg acgttcgttt tcatctggcg
    14281 attgccgaag cctcacataa catcgtgctg ctgcaaacca tgcgcggttt cttcgatgtc
    14341 ctgcaatcct cagtgaagca tagccgtcag cggatgtatc tggtgccacc ggttttttca
    14401 caactgaccg aacaacatca ggctgtcatt gacgccattt ttgctggtga tgccgacggg
    14461 gcgcgtaaag caatgatggc gcaccttagt tttgttcaca ccaccatgaa acgattcgat
    14521 gaagatcagg ctcgccacgc acggattacc cgcctgcccg gtgagcataa tgagcattcg
    14581 agggagaaaa acgcatgatt atttccgcag ccagcgatta tcgcgccgca gcgcaacgca
    14641 ttctgccgcc gttcctgttc cactatatgg atggtggtgc atattctgaa tacacgctgc
    14701 gccgcaacgt ggaagatttg tcagaagtgg cactgcgcca gcgtattctg aaaaacatgt
    14761 ccgacttaag cctggaaacg acgctgttta atgagaaatt gtcgatgccg gtagcactgg
    14821 caccggtggg tttgtgtggc atgtatgcgc gtcgtggcga agttcaggca gccaaagcgg
    14881 cggacgcgca tggcattccg tttactctct cgacggtttc cgtttgcccg attgaagaag
    14941 tcgcgccagc catcaagcgc ccaatgtggt tccagcttta tgtactgcgc gatcgcggct
    15001 ttatgcgtaa cgcgctggag cgagcaaaag cagcgggttg ttcgacgctg gttttcaccg
    15061 tggatatgcc gacaccgggc gcacgctacc gtgatgcgca ttcaggtatg agcggcccaa
    15121 atgcggcaat gcgccgctac ttgcaggcgg tgacgcatcc gcaatgggcg tgggatgtgg
    15181 gcctgaacgg tcgtccacat gatttaggta atatctcagc ttatctcggc aaaccgaccg
    15241 gactggaaga ttacatcggc tggctgggga ataacttcga tccgtccatc tcatggaaag
    15301 accttgagtg gatccgcgat ttctgggatg gcccgatggt gatcaaaggg atcctcgatc
    15361 cggaagatgc gcgcgatgca gtacgttttg gtgctgatgg aattgtggtt tctaaccacg
    15421 gtggccgcca gctggacggt gtactctctt ccgctcgtgc gttgcccgct attgcagatg
    15481 cggtgaaagg tgatatcgcc attctggcgg atagcggaat tcgtaacggg cttgatgtcg
    15541 tgcgtatgat tgcgctcggt gccgacaccg tactgctggg tcgtgctttc ctgtatgcgc
    15601 tggcaacagc gggccaggcg ggtgtagcta acctgctaaa tctgatcgaa aaagagatga
    15661 aagtggcgat gacgctgact ggcgcgaaat cgatcagtga aattacgcaa gattcgctgg
    15721 tgcaggggct gggtaaagag ttgcctacgg cactggctcc gatggcgaaa gggaatgcgg
    15781 catagtcgtt tgcgccccct caccctaacc ctctccctca gggagagggg accgttcggc
    15841 gctgtatgta ctccctcact ctgaaacgac accgcactct ttttttctcc ctcgcccctc
    15901 cggggagggc cggggtgagg ggaaaaggcc gcacggttca ccaattttct gctatcctgc
    15961 ccccgcacta agggggcagt atgctaaaca tcgtacttta cgaaccagaa attccgccaa
    16021 atactggcaa catcatccgt ctttgcgcta ataccggctt tcgtctgcat atcatcgaac
    16081 cgatgggatt tgcctgggac gataagcgcc tgcgccgcgc ggggctggac tatcacgagt
    16141 ttaccgccgt tacgcgtcat catgactatc gcgcgttcct cgaagcagaa aatccccagc
    16201 gcctgtttgc cctcaccacg aaaggtacgc ctgctcacag cgccgtaagc tatcaggatg
    16261 gcgattatct gatgttcggc ccggaaacac gcggcctgcc agcgagcatt cttgatgccc
    16321 tgcccgctga acaaaaaatt cgcattccga tggtgccgga cagccgcagc atgaatctgt
    16381 ccaatgcggt gtcggtagtg gtgtatgaag cctggcggca gctgggatat ccgggagcgt
    16441 tgttgagaga ttagttactg tatgccggat agatgattac atcgcatccg gcacgatcac
    16501 aggacattag atcccatccc catactcaaa tgtatggtta ataccgttga aatgctggtc
    16561 catatccatt gatggcttat cgctgtctgg tttaccgaca atacgagccg gaacgccagc
    16621 ggcggttgta tgcggcggca ccggttgcag caccacggaa cctgcgccaa tcttcgcgcc
    16681 gcgaccaact tcaatattgc cgaggatttt cgcgcccgcg ccaatcatca caccttcacg
    16741 aattttcggg tgacggtcac caccagattt acccgtaccg ccaagcgtca cagattgcag
    16801 aatcgatacg tcgttttcaa ttaccgccgt ttcacccaca acgatgcccg tggcgtggtc
    16861 gagcataatg ccacggccaa tttttgctgc cgggtgaata tcgacctgga acgtcacaga
    16921 aacctggttt tgcagaaaga ttgccaatgc gcgacgcccc tgattccaca accagtgacc
    16981 gatgcgatag gcctgcaagg catgaaaacc cttcaggtat aacaacgggg ttgagtattt
    17041 atcgactgcc gggtcgcggg tacgcaccgc ctgaatatca caggccgcag aggcgatcat
    17101 ttccgggtca gcggcgtagg cttcttccac cacttcacgg atagcaatag caggcataat
    17161 tggcgatgac agcttgttcg ccagcatgta gctcagtgca ctaccaaggt tttcgtgctt
    17221 gagtagcgtc gcgtggtaaa aactggccag cattggctca cagtccgcca gcgttctggc
    17281 ttcggcttta atattgttcc agacaatttc cagttcttca cacgacattg cttactccac
    17341 acgatgagat aatgaccggc ccgttctgcg cgggccgggt catagcggta acaaaggttc
    17401 cctggggtta gtggctgctg cgctcgtcct tgcgtgcacg acctaataac gtcaatgctg
    17461 cctcgcgcgc gttttttccg caatataata cttgataaat ttcctcggtt attggcattt
    17521 ctacgccgaa gcgatgcgcc agttcgcgga cttctttcgt attgcggtag ccttccacca
    17581 cctgaccaat cttctcctgc gcgctttgta catccatgcc ctgaccgagc atcatgccaa
    17641 aacggcggtt acgcgactgg ttgtcggtac aggtaagcac cagatcgccc agccccgcca
    17701 tgcccataaa ggtggcagga tcggcaccca gcgccgcgcc aagacgcgac atttcagcca
    17761 gcccacgggt gatcagtgcc gtacgcgcat tcgcaccaaa accgataccg tcggacatcc
    17821 ccgcgccaat ggcaatgacg tttttcaccg caccgccaag ctgcacaccg atgaaatccg
    17881 ggttgctgta aacgcggaaa cttttgccac agtgcagcaa ttgttggaga tcatcggcaa
    17941 aggtctggtc ggtagaggcc aacgaaatag ccgtcggtaa acctgccgcc agttctttcg
    18001 caaacgttgg gccagagata accgccagcg gaatttgatc gcctaaggcc tcacgcgcca
    18061 cgtcctgtaa cagacgtccg gtttccgctt ccagcccttt ggtcgcccac accagacgcg
    18121 catcaggacg catcagcggt ttaatctggc gcagcacttc accaaagaca tggctgggta
    18181 cgacgacgag aatattacgg ctggctgcca gcgcagtggc gagatcgctt tcaagatgga
    18241 gcgtatcggg aaaaggcaca tcggggagaa acgcggcgtt acagcggtcg cgttcaagcg
    18301 ttgcgatatg ttcagggtca tggccccaga ggacaacctc gtggccattt cttgccaggg
    18361 taatggcaag agcggtgccg tacgagccgg caccgatcac agtcattgaa gcattacgtt
    18421 ggttcatcag gcatcctgat gttcttcagt accttcgcca gcctgctgct gcaaatagtt
    18481 catgaacagc gcatcgaagt taatcggcgc aaggttcagt tgcgggaatg taccgcggga
    18541 taccacgctg gtgatgcact cacgagcata cgggaacaga atgttcgggc agtatgcgcc
    18601 caggcaatgt gccatctggg tgccttcgat acccgcgatg gagaaaatac cgccctgctg
    18661 aacttcacac aggaacgcgg tttcttcgcc cagggaggcc gttacggtaa cacgcagtac
    18721 cacttcgtat acgtcatctg ccagttggga agatgccgta tccagatcaa gtttaacttc
    18781 tggttgccaa tctttctgga aaacgtgcgg cgcgttcggc gcttcgaaag agatatcctt
    18841 ggtataaata cgttggatct ggaaagtcat ttcagtgttg ttttgttctg acatgtgtag
    18901 aaaaccctta agtgttgtcc ttaaatactg cgcaatgtaa tgccaacgtt cacacgttat
    18961 ttcagcaggg gatccagtcc accacgtgca tccaatgcat acaagtcatc acagccgcca
    19021 atgtgctgtg cgtcaataaa aatctgggga accgtggtgc gaccgctgcg tttgatcatc
    19081 tcttcacgct tggcggcatt gccatcgatc ggcaactcct gaaaactcac gcccttgctg
    19141 ctcagcagtg cttttgcacg atggcaatac gggcaggttt ctttggtata gatttcaaca
    19201 ttggccataa tttagctcct gtttttttac cctgaaattt catgttgcag ggcggctgca
    19261 aaatggagtg tccccagaag cttataacaa taagtcgctg ggggcctaat tgcagctaac
    19321 ggcctgcatc atgaaagacg acaggtaaat tatttgccgc gcaccagagg caagttttcg
    19381 ccagcccagc cagcaacgcc ctctttcagc acgaatactt gcgcaaaacc agctttcgtc
    19441 agcgcgtttg caggctcctg gcactgcatg ccagaaccgt ctaccacgat aaccggtttg
    19501 tctttgtgct tctcaagctc accaacattg ttggctttga tttcgctcgg caacagattg
    19561 atagaacctg cgatatggcc tttacggaag tcatcacgct gacgtaaatc cacaaccaca
    19621 gcgtcttctt tgttgatcag acgcgtagct tcaccacgag taatcacctt cactttcgag
    19681 gtcaggctct taaacgtagt cacaagaacc gccaccagta acgcgatcca ggcgatactc
    19741 agtatgggat gacggccaac aaattgcata atttcttgca tggggggtaa caactcccga
    19801 cgtagtgatt aaaaaaacca ggaaaggagt atacctgcgc ggtgcggcaa atacagccag
    19861 cgcgttaact ggaatgcaat tttgcggggc acgacgaaaa aaaagcgcaa atctggcatc
    19921 ggcttgccgc gagcggtcgt atattttgat cttcagaggc tattttatcg attcagctgt
    19981 agtaaaatta cgcaaatttt gactcttgag tatgaggttg tcgcaatgtc ggtttctaaa
    20041 aaacctatgg tactggtgat tctggatgga tatggctatc gtgaagaaca gcaggataac
    20101 gccattttta gtgctaaaac cccggtaatg gatgcactgt gggccaatcg tccgcatacc
    20161 ctgatcgacg cttccggtct ggaagtcggt ctgcctgacc gtcagatggg taactccgaa
    20221 gtaggccacg ttaacctggg tgccggccgc atcgtgtatc aggacctgac tcgtctggac
    20281 gttgaaatca aagatcgcgc tttctttgct aatccagtgc tgactggcgc ggtagataaa
    20341 gcgaaaaacg caggtaaagc agtacacatt atgggtctgc tctccgcagg cggcgtacac
    20401 agccacgaag atcacatcat ggcgatggta gaactggcag ctgaacgcgg cgcagaaaaa
    20461 atctacctgc acgcattcct tgacggtcgc gacaccccgc cgcgcagtgc tgaatcctcg
    20521 ctgaaaaaat ttgaagagaa atttgctgcg ctgggtaaag gccgcgtagc gtccatcatt
    20581 ggtcgttact acgcgatgga ccgcgataac cgttgggatc gcgttgaaaa agcctatgac
    20641 ctgctgactc tggcgcaggg tgagtttcag gccgatactg ccgttgctgg tttgcaggca
    20701 gcttatgctc gcgacgaaaa cgacgagttc gtgaaagcga ccgttattcg tgcagaaggt
    20761 cagccagatg cggcaatgga agacggcgat gcgctgattt tcatgaactt ccgtgctgac
    20821 cgcgcgcgtg aaatcactcg tgcattcgtg aacgctgatt tcgatggctt cgcgcgtaag
    20881 aaagtggtta acgtcgattt cgtgatgctg accgaatacg ccgctgacat caaaactgcg
    20941 gttgcttacc cacccgcttc cctggttaac accttcggcg agtggatggc gaaaaacgac
    21001 aaaactcagt tgcgtatttc cgaaaccgaa aaatatgccc acgttacttt cttcttcaac
    21061 ggtggcgtag aagagtcgtt caaaggcgaa gatcgcattc tgatcaactc accgaaagtg
    21121 gctacctacg atctgcaacc ggaaatgagc tccgcagagc tgaccgaaaa actggttgcg
    21181 gccatcaaga gcggcaaata cgacaccatt atctgtaact atccgaacgg cgacatggta
    21241 ggtcacaccg gggtgatgga agcggcggtt aaagcggttg aagcactgga tcactgcgtg
    21301 gaagaagtcg cgaaagcggt tgagtccgtg ggtggacaac tgctgatcac cgctgaccac
    21361 ggtaacgctg aacagatgcg cgatccggca acgggtcagg cacacacggc acacaccaac
    21421 ctgccagttc cgctgattta cgttggtgat aagaacgtga aagcggttgc aggcggcaaa
    21481 ctttctgaca tcgcgccgac catgttgtcg ctgatgggta tggaaatccc gcaagagatg
    21541 actggtaagc cgctgttcat cgtggaataa tccctcccca tgaggggaaa ggcgattaat
    21601 accatgacac gggccgtgaa accgcgcagg tttgcaatca ggcccatcat ctacgccagc
    21661 gttcttagcg ctggcgtatt gttgtgcgcc ttttccgccc acgcggatga gcgtgaccaa
    21721 ctcaaatcta ttcaggccga tatcgccaca aaagagcgcg cggtacgcca aaagcaacaa
    21781 caacgcgcaa gcctgctcgc acaattgaaa aagcaggaag aagcgatctc tgaagccacc
    21841 cgtaagctgc gcgaaacgca aaacacgctc aatcaactca ataaacagat tgatgagatg
    21901 aacgcgtcga ttgccaaact ggagcagcaa aaagccgccc aggagcgcag cctcgccgca
    21961 caactggatg ccgcattccg tcagggcgag cataccggta ttcagctaat tctcagcggt
    22021 gaagaaagcc agcgtggaca gcgtttacag gcttatttcg gctatctcaa ccaggcgcga
    22081 caagaaacca ttgcccagtt gaagcaaacg cgtgaagaag tcgccatgca gcgtgctgaa
    22141 ctggaagaga aacaaagcga gcaacaaacg ctgttatatg aacagcgcgc ccaacaggcg
    22201 aaactgactc aggctctgaa cgagcgtaaa aagacgctgg cggggctgga atcttccatc
    22261 cagcaaggtc agcagcagct aagtgaactg cgtgccaacg aatcccgtct gcgtaacagt
    22321 attgcccgtg cggaagccgc ggcgaaagcg cgtgctgaac gagaagcgcg tgaggctcag
    22381 gcagttcgcg acaggcagaa agaagcgacg cgcaaaggca ccacctacaa accgaccgaa
    22441 agcgaaaaat cgctgatgtc ccgaactggt ggcctgggtg cgccgcgtgg tcaggcattc
    22501 tggccagttc gtggccctac gctgcatcgc tatggcgagc agctacaggg tgaattacgc
    22561 tggaaaggaa tggttatcgg tgcctctgaa ggtactgaag ttaaagcaat tgccgacggt
    22621 cgggtgattc tggctgactg gctgcaaggc tacggtctgg tggtggtggt tgagcacggt
    22681 aaaggcgata tgagtcttta cggctataat caaagcgcgc tggtgagcgt tggttcgcag
    22741 gttcgtgcgg gccagccaat tgcactggtg ggcagcagtg gcggtcaggg cagaccttca
    22801 ctctatttcg aaattcgccg ccagggtcag gccgtcaatc cacagccgtg gttgggaaga
    22861 taagttttgt ttccatttcg tcgtaacgtt cttgcatttg ccgctctgtt ggcgctctcc
    22921 tcccctgtaa ttgctggcaa acttgccatc gtcattgatg attttgggta tcgcccgaac
    22981 aacgaaaacc aggtgctggc gatgccttcc gctatctccg tcgctgtatt acccgattca
    23041 ccgcacgcca gagagatggc gaccaaagcg cataacagcg gtcacgaagt gttgattcat
    23101 ctcccgatgg caccgttgag taaacagccg ctggagaaaa atacgctgcg cccggagatg
    23161 agcagcgacg aaattgagcg cattattcgt agtgcggtca ataacgtgcc ctatgccgtg
    23221 gggatcaata accacatggg cagcaagatg acctctaacc tgtttggtat gcagaaagtg
    23281 atgcaggcgc tggagcgtta taatctttac ttcctcgaca gcgtaaccat tggtaatacc
    23341 caggcgatgc gcgccgcgca aggcactggc gtgaaggtga tcaaacggaa ggtgttcctc
    23401 gacgattcac agaacgaagc ggacatccgt gtgcaattca atcgcgcaat tgacctagcg
    23461 cgtcgcaacg gatcaaccat tgccattggg catcctcacc cttcaacggt acgcgtgttg
    23521 caacagatgg tttataacct gccgccagac attacgctgg tgaaagccag cagcttgctg
    23581 aatgaaccgc aggttgatac gtctacaccg ccgaaaaaca ctgtgcccga cacaccgcgt
    23641 aatccgttcc gtggcgtgaa gctgtgcaaa ccgaagaaac cgatagaacc tgtttatgct
    23701 aatcgcttct ttgaagtatt aagcgaaagc atcagccaga gcacgctgat cgtttacttc
    23761 cagcatcagt ggcaaggctg gggcaaacag cctgaagcgg cgaagcttaa cgctagcgta
    23821 aattaaaggc gctacgcgta atgcgatggt gcgaccgcgt tttgtcgcgc cattgccata
    23881 agcggaaaga ctacagtaac gcctgataac ccacctttac gctgcgcaca ttggtaatca
    23941 ggcgcttata ca
//
LOCUS       DAJVRF010000055        23776 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJVRF010000055 DAJVRF010000000
VERSION     DAJVRF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.055"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            103..1329
                     /gene="eutH"
                     /locus_tag="OQN13_001985"
     CDS             103..1329
                     /gene="eutH"
                     /locus_tag="OQN13_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HCS1615351.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            1326..2729
                     /gene="eutA"
                     /locus_tag="OQN13_001986"
     CDS             1326..2729
                     /gene="eutA"
                     /locus_tag="OQN13_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCS1615352.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            2741..4102
                     /gene="eutB"
                     /locus_tag="OQN13_001987"
                     /pseudo
     CDS             2741..4102
                     /gene="eutB"
                     /locus_tag="OQN13_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
     gene            4123..5010
                     /gene="eutC"
                     /locus_tag="OQN13_001988"
     CDS             4123..5010
                     /gene="eutC"
                     /locus_tag="OQN13_001988"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCS1615353.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            5020..5679
                     /gene="eutL"
                     /locus_tag="OQN13_001989"
     CDS             5020..5679
                     /gene="eutL"
                     /locus_tag="OQN13_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCS1615354.1"
                     /translation="MPALDLIRPSVTAMRVIASVNSEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            5692..6192
                     /gene="eutK"
                     /locus_tag="OQN13_001990"
     CDS             5692..6192
                     /gene="eutK"
                     /locus_tag="OQN13_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCS1615355.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            6238..7290
                     /gene="eutR"
                     /locus_tag="OQN13_001991"
     CDS             6238..7290
                     /gene="eutR"
                     /locus_tag="OQN13_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCS1615356.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(7296..8195)
                     /gene="hemF"
                     /locus_tag="OQN13_001992"
     CDS             complement(7296..8195)
                     /gene="hemF"
                     /locus_tag="OQN13_001992"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCS1615357.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(8199..9068)
                     /gene="amiA"
                     /locus_tag="OQN13_001993"
     CDS             complement(8199..9068)
                     /gene="amiA"
                     /locus_tag="OQN13_001993"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCS1615358.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTVAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            9282..9707
                     /locus_tag="OQN13_001994"
     CDS             9282..9707
                     /locus_tag="OQN13_001994"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCS1615359.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            9694..10143
                     /locus_tag="OQN13_001995"
     CDS             9694..10143
                     /locus_tag="OQN13_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCS1615360.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            10204..10779
                     /locus_tag="OQN13_001996"
     CDS             10204..10779
                     /locus_tag="OQN13_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCS1615361.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            10875..11774
                     /gene="yfeX"
                     /locus_tag="OQN13_001997"
     CDS             10875..11774
                     /gene="yfeX"
                     /locus_tag="OQN13_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCS1615362.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(11832..13136)
                     /gene="pbp4b"
                     /locus_tag="OQN13_001998"
     CDS             complement(11832..13136)
                     /gene="pbp4b"
                     /locus_tag="OQN13_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HCS1615363.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(13141..13314)
                     /locus_tag="OQN13_001999"
     CDS             complement(13141..13314)
                     /locus_tag="OQN13_001999"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HCS1615364.1"
                     /translation="MGLNSAFGPSGLVALPLMTSAQGILPAMAVYAGGILVAWVCGFI
                     FTTLFGCRNVNLD"
     gene            complement(13327..14565)
                     /gene="murP"
                     /locus_tag="OQN13_002000"
                     /pseudo
     CDS             complement(13327..14565)
                     /gene="murP"
                     /locus_tag="OQN13_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040446.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            complement(14569..15465)
                     /gene="murQ"
                     /locus_tag="OQN13_002001"
     CDS             complement(14569..15465)
                     /gene="murQ"
                     /locus_tag="OQN13_002001"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCS1615365.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            15629..16486
                     /gene="murR"
                     /locus_tag="OQN13_002002"
     CDS             15629..16486
                     /gene="murR"
                     /locus_tag="OQN13_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCS1615366.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            16615..17406
                     /gene="ucpA"
                     /locus_tag="OQN13_002003"
     CDS             16615..17406
                     /gene="ucpA"
                     /locus_tag="OQN13_002003"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCS1615367.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            17577..18593
                     /gene="cysP"
                     /locus_tag="OQN13_002004"
     CDS             17577..18593
                     /gene="cysP"
                     /locus_tag="OQN13_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCS1615368.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            18593..19426
                     /gene="cysT"
                     /locus_tag="OQN13_002005"
     CDS             18593..19426
                     /gene="cysT"
                     /locus_tag="OQN13_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCS1615369.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            19426..20301
                     /gene="cysW"
                     /locus_tag="OQN13_002006"
     CDS             19426..20301
                     /gene="cysW"
                     /locus_tag="OQN13_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCS1615370.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            20291..21388
                     /gene="cysA"
                     /locus_tag="OQN13_002007"
     CDS             20291..21388
                     /gene="cysA"
                     /locus_tag="OQN13_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCS1615371.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            21522..22433
                     /gene="cysM"
                     /locus_tag="OQN13_002008"
     CDS             21522..22433
                     /gene="cysM"
                     /locus_tag="OQN13_002008"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCS1615372.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(22436..22804)
                     /locus_tag="OQN13_002009"
     CDS             complement(22436..22804)
                     /locus_tag="OQN13_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCS1615373.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            22909..23760
                     /gene="pdxK"
                     /locus_tag="OQN13_002010"
                     /gene_synonym="thiJ"
     CDS             22909..23760
                     /gene="pdxK"
                     /locus_tag="OQN13_002010"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCS1615374.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT         5415 a         6100 c         6759 g         5502 t
ORIGIN      
        1 gcttgccgcg tctgatccgg ccttcgggat tgcacatgta gggcggataa ggcgtttacg
       61 ccgcatccgc caataaataa caactaacag ggagtaaagg cgatgggaat taacgaaatc
      121 atcatgtaca tcatgatgtt ctttatgctg atagctgccg tagacaggat cctgtcgcag
      181 ttcggcggtt ctgctcgttt cctcggtaag ttcggtaaaa gtatcgaagg atcaggcggt
      241 cagttcgaag aaggctttat ggcaatgggc gctctgggtc tggcgatggt cggtatgacc
      301 gcgctggcac cggtactggc tcacgtactc gggccggtaa ttatcccggt ttacgaaatg
      361 ctcggcgcaa acccgtcaat gttcgccgga acgctgctgg cgtgcgatat gggcggcttc
      421 ttcctcgcca aagagctggc gggcggcgac gtagcagcgt ggctatactc tgggttaatt
      481 cttgggtcga tgatggggcc aacgattgtg ttttccattc cggtggcgct cggcattatc
      541 gaaccttctg accgtcgtta tctggcgctc ggcgtgctgg cgggcattgt gaccattccg
      601 attggctgta ttgctggtgg tctggttgct atgtactccg gtgtgcagat caacggtcag
      661 ccggtggaat tcactttcgc cctgatcctg atgaacatga tcccggtgat cattgttgcg
      721 attctggtgg cgctggggct gaaattcatc ccggaaaaaa tgatcaacgg cttccagatc
      781 ttcgccaaat tcctcgttgc attgatcacc ctcggtcttg ctgccgcggt agttaagttt
      841 ctgcttggct gggaactgat ccccggtctg gaccctatct ttatggcccc tggcgataaa
      901 cccggtgaag tgatgcgcgc cattgaagtt atcggttcta tctcctgcgt tctgttaggg
      961 gcgtatccaa tggtgctgct gctgacccgc tggtttgaaa aaccgctgat gagcgtcggt
     1021 aaagtgctga atatgaacaa catcgcggca gccggcatgg tggcaacgct tgccaacaac
     1081 atcccgatgt tcggcatgat gaagcagatg gatacccgcg gcaaagtcat caactgcgcc
     1141 ttcgccgttt ccgctgcttt cgccctgggc gaccacttag gcttcgccgc tgccaacatg
     1201 aacgccatga tcttcccgat gattgtcggc aagttgatcg gcggcgtaac ggcgattggc
     1261 gtggcgatga tgctggtgcc aaaagaagac gcgaccacgg ctaaaaccga agcggaggca
     1321 caatcgtgaa cactcgccag ctattgagcg tcggtatcga tatcggcacc accaccaccc
     1381 aggtgatttt ctcccacctg gagctggtta accgtgcggc ggtgtcgcag gtgccgcgct
     1441 acgaattcat taaacgcgaa attagctggc aaagcccggt gttctttacc cctgtcgata
     1501 aacagggtgg tttaaaagaa gcggaactga aaaccttaat cctcgagcaa tatcaggctg
     1561 cgggcattgc gccggaaagc gttgattctg gtgccatcat catcaccggt gaaagcgcga
     1621 aaacccgcaa tgctcgtccg gcggtgatgg tgctctccca gtcgctgggc gattttgtcg
     1681 ttgccagcgc cgggccgcac ctcgaatccg tcattgccgg tcacggtgcc ggggcacaaa
     1741 ccctttctga acaacggctg tgtcgggtac tgaatattga catcggcggt ggcactgcga
     1801 actacgccct gttcgatgcc ggaaaaatca gcggcactgc ctgcctcaac gtcggtggtc
     1861 gcctgctgga aaccgacagc caggggcgcg tggtttacgc tcataaaccg gggcagatga
     1921 ttgtggatga gtgcttcggc gcaggcaccg acgcccgttc gctgaccggc gcgcagctgg
     1981 tgcaggttac ccggcggatg gcagaactga ttgtcgaagt gattgacgga acgctctcgc
     2041 cgctcgcgca ggcattgatg caaaccggtt tgctgcccgc aggtgttacg cccgaaatca
     2101 ttacgctttc tggcggcgtg ggcgaatgtt atcgccacca gcccgccgac ccgttctgtt
     2161 ttgccgatat tggcccgctg ctggcaacgg cgctgcatga ccatccgcgc ctgcgtgaga
     2221 tgaacgtaca gtttccggcg caaaccgtgc gcgccacggt gattggcgcg ggcgcgcata
     2281 ccctttcgct ctctggcagc acaatctggc ttgagggcgt acaactgccg ctgcgcaatt
     2341 tgccggtggc gatcccgatt gatgaaacgg atctggtgag tgcctggcaa caggcgctgc
     2401 ttcagctgga tcttgatccc aaaactgacg cgtacgtgct ggcgcttccc gcctcgctgc
     2461 ctgtgcgtta cgccgcggta ctgacggtca tcaacgcgct ggtcgatttc gtcgcgcgtt
     2521 ttccaaatcc gcatcccctg ctggtggtgg ccgggcagga ctttggtaaa gctctgggca
     2581 tgttgttgcg cccacagcta caacaactcc cgttggcagt cattgacgaa gtgattgtcc
     2641 gcgcggggga ctatatcgac attggtacgc ctctttttgg cggatcggtt gtgccggtga
     2701 cggtgaaatc actcgcattt ccttcctgag ggaacgactt atgaaactaa agaccacatt
     2761 gttcggcaat gtatatcagt ttaaggatgt aaaagaggtg ctggctaaag ccaacgaact
     2821 gcgttcgggg gatgtgctgg cgggcgttgc agcggcaagc tcacaggagc gcgtggcggc
     2881 aaagcaggtg ttgtcggaaa tgaccgtagc ggacatccgc aataatccgg tgattgccta
     2941 tgaagatgac tgcgtgacgc ggctgattca ggacgatgtt aacgaaacgg cctacaacca
     3001 gattaaaaac aggagcatca gcgaactgcg tgagtatgtg ctgagcgatg aaaccagcgt
     3061 ggacgacatt gcctttaccc gcaaagggct gacctcggaa gtggtcgcgg cggtagcgaa
     3121 gatttgctcc aacgcggacc tgatctacgg cgcgaagaaa atgccggtaa tcaaaaaggc
     3181 caataccacc atcggtattc cgggcacctt cagcgcccgt ttgcagccaa atgacacccg
     3241 tgacgacgtg caaagtatcg ctgcacaaat ctacgaaggg ctttccttcg gggtgggcga
     3301 tgcggtgatc ggcgttaacc cggtaactga cgacgtggaa aacttaagcc gcgtgttgga
     3361 caccatttac ggcgtgatcg acaaattcaa catcccaact cagggctgcg tactggcgca
     3421 cgtcaccacc cagatcgaag cgattcgtcg cggcgcgcct ggcggactga ttttccagag
     3481 tatctgtggc agcgaaaaag ggctgaaaga gtttggcgtg gaactggcga tgctcgacga
     3541 agcgcgcgca gtgggtgctg agttcaaccg tatcgccggg gaaaactgcc tctacttcga
     3601 aaccgggcaa ggttcggcgc tgtctgctgg cgctaacttc ggcgcagact aggtgacgat
     3661 ggaagcacgt aactatgggc tggcgcgtca ttacgatccg tttatcgtca acaccgtggt
     3721 cggctttatt gggccggagt atctctacaa cgaccgccag attatccgtg ctggcttaga
     3781 agatcacttt atgggcaagc tgagcggcat ctctatgggc tgtgactgct gttacaccaa
     3841 ccacgctgat gctgaccaga acctcaacga aaacctgatg atcctgctcg ccaccgcagg
     3901 ctgcaactac atcatgggga tgccgctggg cgatgacatc atgctcaact atcagaccac
     3961 cgcattccac gacactgcca ctgtgcgtca gttactcaac ctgcgcccgt caccggagtt
     4021 tgaacgctgg ctggaaagca tgggcattat ggcaaacggt cgcctgacca aacgggcggg
     4081 cgatccgtca ctgttcttct gatgacgcgg agataacaca tcatggatca aaaacagatt
     4141 gaagaaattg tacgcagcgt gatggcgtca atgggacaag cggcccccgc gccgtcagaa
     4201 gcaaagtgcg ccaccactaa ctgtgcggca ccggtgacct cggaaagctg cgcgctggat
     4261 ttaggttctg cggaagcaaa agcgtggatt ggtgttgaaa acccgcatcg cgcagacgta
     4321 ttaacagaac tgcgccgcag caccgtggcc cgcgtttgta ccggtcgtac cggtccgcgt
     4381 ccacgtacgc aggcgctgct gcgtttcctg gccgatcact ctcgttcgaa agataccgta
     4441 ctgaaagaag tgccggaaga gtgggtgaaa gcgcagggct tgctggaagt acgctccgaa
     4501 attagcgaca aaaatctcta cctgactcgc ccggatatgg gccgccgcct gtgtgcagaa
     4561 gctgttgaag cgctgaaagc acagtgtgtt gctaatccgg acgtacaggt cgttatttct
     4621 gatggcctgt caactgatgc catcaccgtg aactacgaag agatcctgcc gccgctgatg
     4681 gcgggcctga aacaggccgg gctgaaagtc ggcacgccat tctttgttcg ttatggtcgc
     4741 gtgaagattg aagatcagat cggtgagatc ctcggcgcga aagtggtgat cctgctggtg
     4801 ggggaacgtc cggggttggg tcagtcagaa agcctctcct gttacgccgt ttactcgccg
     4861 cgtatggcga ccaccgttga ggccgatcgc acctgcatct ctaacattca ccagggcggc
     4921 acgccgccgg ttgaagccgc agccgtcatt gtggatttgg cgaaacgtat gctggagcag
     4981 aaagcatccg gcatcaacat gacccgataa ggaggcatca tgccagcttt agatttgatt
     5041 cgaccgtcgg taaccgctat gcgggtgatt gcctccgtta actctgaatt tgcgcgtgaa
     5101 ctaaaattgc cgccgcatat tcgtagcctc gggctgattt ctgctgattc cgatgacgtc
     5161 acctacattg cggctgacga agcgaccaag caggcgatgg tcgaagtggt atatggccgc
     5221 tcgctgtatg cgggagcagc acacggaccg tcaccgactg ccggtgaagt gctgattatg
     5281 cttggtgggc caaacccggc ggaagtgcgt gctggtctgg atgcgatggt tgcgcatatt
     5341 gaaaatggcg ctgctttcca gtgggctaat gacgcagaaa acaccgcatt cctggcacat
     5401 gtagtttcgc gtaccggttc ttatctttca tcaaccgccg ggatcacgct tggcgatccg
     5461 atggcgtatc tggtggcacc gccgctggaa gcgacctacg gcattgatgc agcgttgaaa
     5521 tctgccgacg tacagctggt gacctatgtc ccgccaccgt ctgaaaccaa ctactcggca
     5581 gcatttttaa ccggtagcca ggccgcgtgt aaagcagcct gtaacgcctt taccgatgca
     5641 gtgctggaaa tcgcgcgtaa tccaatccag cgtgcgtaac ggaggttgcc gatgatcaat
     5701 gcactgggat tgctggaagt ggacggcatg gtcgccgcga tagatgcagc ggatgccatg
     5761 ctcaaagcag ctaacgttcg tctgctcagt cacgaagtgc ttgaccctgg tcgcttaacg
     5821 ctggtggtgg aaggcgatct ggcggcgtgt cgtgcggcgc tggacgcagg ttgtgctgcc
     5881 gcgatgcgta ccgggcgtgt catcagccgc aaggagatcg gtcggccaga cgatgacacc
     5941 cagtggctgg ttactggctt taaccgccag ccgaagcaac ccgtaaagga acccgacgcg
     6001 ccagttatcg tcgcggaatc tgctgacgag ttgttggcgc tgttaacatc agtacgtcag
     6061 ggaatgacgg caggagaagt ggctgcccac tttggctggc cgctggaaaa agccagaaat
     6121 gcgctcgaac agctcttttc tgccgggacg ttacgtaaac gcagtagtcg ttatcgtctc
     6181 aagccccatt aacctgtcgg aggtgccggg tgtcctacaa cacccggcat taacatcatg
     6241 aaaaagaccc gtacagccaa tttgcaccat ctttatcatg aacccttacc cgaaaacctg
     6301 aagctcacgc cgaaggtcga agtggataat gttcatcaac gacagacaac ggatgtctat
     6361 gaacatgctt tgacgattac cgcctggcag cagatttacg atcagctgca tccgggcaag
     6421 tttcatggtg aatttacgga aattctgctc gatgatattc aggtttttcg tgaatacacc
     6481 ggtctggcgc tgcgtcagtc gtgcctggtc tggccgaact cgttctggtt tggcattccg
     6541 gcgacgcgcg gtgagcaggg atttatcggt tcgcaatgtc tgggaagcgc agaaatagcg
     6601 acgcgccccg gtggaaccga gtttgaatta agtacgccgg acgattatac gatcctgggc
     6661 gtagtgctgt ctgaagatgt catcacccgt caggctaact ttttgcacaa tccggatcgg
     6721 gtattacata tgctgcgtag ccagtcggcg ctggaagtga aagagcagca taaagccgcg
     6781 ctgtggggct ttgtccaaca ggcgctggcg acgttttgcg agaacccgga aaatctccat
     6841 cagcctgcag tgcgaaaagt gctgggggat aatttgctaa tggcgatggg ggcgatgctg
     6901 gaagacgcgc aaccaatggt gacggcggaa agcatcagtc atcagagtta ccgtagatta
     6961 ctttcccgcg cccgtgaata tgtgctggaa aatatgtctg agctggtgac ggtgctggac
     7021 ttgtgtaatc aactgcatgt cagtcgccgc acgctacaaa acgcgtttca cgctatttta
     7081 ggcattggcc caaacgcgtg gctgaaacgc attcgcctga acgccgtacg ccgcgaactg
     7141 ataagcccgt ggtcgcaaag cacaacggta aaagacgccg ccatgcagtg gggattctgg
     7201 catctggggc aatttgccac ggattaccag cagctgtttg ccgagaagcc gtcgttgacg
     7261 ttgcatcagc ggatgcggga atgggggtga gggagttaca cccaatccct gaccttaata
     7321 aactcactta acgccgcttc tgggctgcca tcttttggct gataatcata ctcccagcgt
     7381 accagtggcg gcattgacat caggatcgac tcggtgcgcc cgccagtttg caggccaaac
     7441 agagtgccgc gatcccagac cagattgaac tcgacataac gaccgcgacg gtagagctga
     7501 aaattgcgct cgcgctcgcc gtaggccatc gctttacgtc gctctacaat tggtaaataa
     7561 gcgtcggtgt agcctttgcc taccgcctgc ataaaggcaa aacagtggtc gaaatctggc
     7621 gtgttcagat catcaaagaa cagcccgcca ataccgcgct gttcgttgcg atgtttgagg
     7681 tagaagtaat cgtcgcacca ctttttgtaa cggggataaa cgtcttcacc aaatggcagg
     7741 cacaggtcac gggcggtgcg gtgccagtga atggcgtctt cttcaaaacc atagaaaggg
     7801 gttaaatcga agccgccgcc aaaccaccag acgggctctg cacccggttt ttcggcaata
     7861 aaaaaacgca cattcgcgtg gctggtggga acatacgggt tatgcggatg cactaccagt
     7921 gaaacgccca tcgcctcgaa actgcgcccg gcaagttccg ggcgatgagc ggtggcggaa
     7981 gcaggcatcg cctcgccgtg gacgtgagaa aagttgacgc ctgcctgttc gaaaacacca
     8041 ccattacgca acacccgact acgcccgccg ccgccagctt cgcgctgcca actatcttcg
     8101 acaaattctg cgccatcgac ggcggtcagc tgctgacaaa tcgtatcctg aaggttgagc
     8161 agaaactgtt taacctggtg tgcgtcgggt ttcataactt atcgcttttt cgaatgtgct
     8221 ttctggttgt cgaaccagtg gaaataactg atcacgcctt cagcaatcgc tgtggcgatt
     8281 ttctgacgaa acgccaccgt gcctaacagc cgctcttctt ccgggttggt gataaacgag
     8341 gtttccacca gcaccgaagg aaccgacggc gatttcaaca ccacaaacgc ggcttgttcg
     8401 gtgttgcggc tgtgcagttt atgcaccggc ttaatctttt tcagaatatg cgagccaagc
     8461 gtcaggctgt ttttaatagt atcggtttgc accagatcaa acagcacttg ctgcaatagg
     8521 tgatccttgt cagtcgcctt tttaccggca acttcatcgg cgcggttttc gcgttcagac
     8581 aggtatttcg ccattgcgct acttgcccca cggttagaga gggcaaatac cgaagcaccg
     8641 gcagctttcg ggttggtaaa gccatcggca tgaattgaca taaacagatc tgcgccatgt
     8701 ttatgggcga tttcaacgcg atcgtaaagt gggataaacg tatcgccaga acgcgttaaa
     8761 cgcgcatcaa tcccatgatt acgcaaaatg gaacggacgt ttttagcaat cgccagcacc
     8821 acatgttttt ctttcgaacc gttgcgtccg atcgctccgg tatcaattcc gccgtggcct
     8881 ggatcgagaa caacgacacg tttgccgcca gattttttgg ctttcggctt gctgtgtccg
     8941 ttgctggttt ttaaaggttc gtctttggcg atggcttgcg acattcctga caacgtcagg
     9001 gcagccaaac cggctttcag cacctggcgg cgcgaagtga gtgtttttag tggtttaaaa
     9061 gtgctcatac ggcctgagtt gtaataataa agttccagat gttatatcgt atcgcgtaat
     9121 ccgttacgac ggtttgatgt tgagaattgt tttacttttc atttcaatag ttgacaactc
     9181 cccattatgc cactttttca accgattttc gtcagatatt ggctaattcg accgttcacg
     9241 ccataatcag ccttttaacc cgcaaaaagg taacgaatac catggagata cgcgtatttc
     9301 gccaggaaga tttcgaagag gtcatcaccc tttgggagcg ttgcgacttg ctgcgtccgt
     9361 ggaacgatcc ggaaatggac atcgagcgta agatgaacca tgacgtcagt ttgtttttgg
     9421 tcgctgaggt aaacggtgaa gtggtcggaa cggtgatggg cggttatgac gggcatcgcg
     9481 ggtctgctta ttatcttggc gtgcatccag agtttcgtgg gcgtgggatt gccaatgcgt
     9541 tgcttaatcg gctggagaaa aagctgattg ctcgtggctg cccgaaaatt cagatcaatg
     9601 tgccggaaga taacgacatg gtgctcggaa tgtatgaacg cctgggatat gaacacgccg
     9661 acgtgctgag tctgggtaag cgtttgattg aagatgaaga gtactgagtt ccatcctgtc
     9721 cattatgatg cgcacggacg cctgcgttta cccttgctct tctggcttgt gctattgctt
     9781 caggcgcgaa cctgggtgct gtttgtcatt gccggtgcgt cgcgcgagca gggtactgcg
     9841 ctgctaaatc tgttttatcc cgatcacgat aatttctggc tggggctgat tccaggcatt
     9901 ccggcggtgc tggcgttttt gttgagcggt cggcgggcta catttcctcg cacttggcat
     9961 gtgctctatt ttctgttgct gttggcgcag gtggttttac tttgctggca accgtggctg
    10021 tggctgaacg gtgaatccgt tagcggtatc ggtctggcgc tggtagtggc ggatattgtg
    10081 gcgttaatct ggctgctgac caatcgacgt ttacgcgctt gttttaatga agaaaaagaa
    10141 taacggcact ttttggtgaa tttgcactcc aagcaacgtt attgaataac caaaggaagt
    10201 gacatgaaat cgctgcgttt aatgttatgc gctatgccgt tgatgctgac cggctgttcc
    10261 acgatgtcgt cagttaactg gtcggccgct aacccgtgga actggtttgg atcgtccacc
    10321 aaagtgagcg agcagggcgt gggtgaatta acggcgtcca caccactgca agaacaagcc
    10381 attgctgatg cgcttgatgg cgattatcgc ctgcgcagcg gaatgaaaac cgcgaacggc
    10441 aacgtggtgc gcttttttga agtgatgaaa ggcgacaacg tggcgatggt gattaacggc
    10501 gatcaaggca cgatcagtcg cattgacgtg ctggatagcg atattcctgc tgacaccggt
    10561 gttaaaatcg gtacaccgtt tagcgacctt tacagcaaag catttggcaa ttgccaaaaa
    10621 gctgatggtg atgataatcg tgccgtcgaa tgtaaagccg aaggcagtca acatattagc
    10681 taccagttca gcggggaatg gcgtggtcct gaagggttaa tgccttcgga cgataccctg
    10741 aaaaactgga aagtcagtaa aattatctgg cggcgttaat ttgcgtctga acaaaccgcc
    10801 gccgcgaaaa aacacgtaaa atagcgctca acaatgccac ggattgcgtg gcattcttat
    10861 tttcaggagg aacaatgtct caggttcaga gtggcatttt gccagaacat tgccgcgcgg
    10921 cgatttggat cgaagccaac gtgaaagggg aagttgacgc cctgcgtgcg gccagtaaaa
    10981 catttgccga caaactggca acttttgaag cgaaattccc ggacgcgcat cttggtgcgg
    11041 tggttgcctt tggtaacaac acctggcgcg ctctgagcgg cggcgttggg gcagaagagc
    11101 tgaaagattt tccgggctac ggtaaaggcc ttgcgccgac cacccagttc gatgtgttga
    11161 tccacattct ttctctgcgt cacgacgtaa acttctctgt cgcccaggcg gcgatggaag
    11221 cctttggtga ctgcattgaa gtgaaagaag agatccacgg cttccgttgg gtggaagagc
    11281 gtgacctgag cggctttgtt gacggtacag aaaacccggc gggtgaagag acgcgtcgcg
    11341 aagtggcggt tatcaaagac ggcgtggatg ccggcggcag ctatgtgttt gtgcagcgtt
    11401 gggagcacaa cctgaagcag ctcaaccgga tgagcgttca cgatcaggag atgatgatcg
    11461 ggcgcaccaa agaggccaac gaagagattg acggcgacga acgtccggaa acctctcacc
    11521 tcacccgcgt tgatctgaaa gaagatggca aagggctgaa gattgttcgc cagagcctgc
    11581 cgtacggcac cgccagtggc actcacggtc tgtacttctg cgcctactgc gcgcgtctgc
    11641 ataacattga gcagcaactg ctgagcatgt ttggcgatac cgatggtaag cgtgatgcga
    11701 tgttgcgttt caccaaaccg gtaaccggcg gctattactt cgcgccgtcg ctggacaagt
    11761 taatggcgtt gtaagcgttt ctgattcgat gattaaaggc cagccagtaa caaaactgac
    11821 tggccttttt attacttttg cttcaacgcc gcatacacct gatcaactac ccaaccataa
    11881 gttgcaatcg gtaactgacc gctttcgaac atattgggat ttttttgtgg atcggcaact
    11941 ggcgaatggg gcttgttgct taacatcaca atcgccatat gattgaccgg atcgataacg
    12001 gttactgtcc cggtccagcc agtgtgaccg taggtttgcg ggcttgccag cgtgccaaac
    12061 gtcggcgtca tggtggcatt accattcacg cgccagccga ggccaaaagt ggcatcttcc
    12121 ttagagctgg tggtgaacat cttcaccgtt tccgcactga acagctgcac atcgccatag
    12181 cccccgccgt tcagcatcgt ttgcattaac accgcaatat cgccggtatt ggaaaacaaa
    12241 cctgcgtgcc cggaaacgcc gcccatcgaa taaaaggctt tttcatcgtg cacctgaccc
    12301 cagagagtgg aggtgcggat attcgggaaa tggatcacgc catcgcgggt attgccgttc
    12361 aattccgtgg cggcaatttg ttgcggttta aagcctttca gcagcgggtt aaataccgta
    12421 tgtgtcaggc cgagcgggcg ataaatcgac tcttcaacat agcggtcaag cggctgaccg
    12481 gtaaccgact cgacgataaa tccaagcagc atataatcga catcgctgta gatatgtttg
    12541 ctgccgggct gatattccag cggcgtgcgc ttgatcatct ccagcgtctg gcctttatcc
    12601 tgggaatata acgcgcccgc gaccgcttta ttcgggtatt gcggatctgc cgggaaaccg
    12661 ccgctgtgat gcagcaggtc agaaatccgc agggtgtttt tgcctttaat ggtgtcattt
    12721 ggactatcgg caaaccccgg aatatatttc gcaatccgat cgtcaggatg cagtttgcct
    12781 tcgctcatca gcttttgcag ggcgaagttc gtggcgtaca ttttggtgtt tgaggccagg
    12841 tcatacagcg tcccggtggt ggctttgaca ggctgttcca tcaacacgct gccatcgtac
    12901 tttttcgccg ctccccaggc tttgcgatac acaatctgat tatctttaat gatcagcagg
    12961 tttacgccgg gataaccgac atcaatttgc tggctaatcc agcgatccat ctggttaagc
    13021 cgttcgacgt taaaccctgc tttctctggc gagctttctg tcagaacagg gtattttgcg
    13081 gcgctgacgc tacaactaac agccagcaga gaaagataga gcattgtccg tttcatgata
    13141 tcagtccaga ttgacgttac ggcagccaaa gagcgtggtg aaaataaacc cgcaaaccca
    13201 tgccaccaga atcccgccag cataaaccgc catggccgga aggatgcctt gtgcggaagt
    13261 catcagcggc agcgctacca gaccagacgg cccaaaggcg ctgtttaagc ccatcggcag
    13321 accccatcag gctatcaggc cgataaacaa accgcccgcc gcgccgccta aacaggcggt
    13381 aacaaacggc ttcatgcggg gcagggtgac accgtaaatc agcggttcac caacgcccag
    13441 caggccggga ataatcgccc cgcgtacctg actgcgtagc gcactgtgcg gttgcgcccg
    13501 ccagtagagt gccagcgccg cgcccacctg gcccgcgcct gccattgaaa ggatgggaaa
    13561 taagctgttg aatccctagc tgtccattaa cgcgaggtaa acaggaataa agccctgatg
    13621 cacgccaaat accacggcga tcaggaacag cccggctaaa accgcacaac cgaacggatt
    13681 actgttcagg tgcataaaca gccatgacat accttcgaat aaccagccac ccagcggcat
    13741 aatgatcagg tacgcaagcg tagcggtgat cagcagggtg attaacgagg tcagcagcat
    13801 gtcgagatcg tccgacataa agcggcgcac catgccttca atgcgggcgc aggcccacgc
    13861 ggcaatcagc acgccgataa tattgccgcg cggatcgatg ggcagaccaa agaaatcgtg
    13921 aaaaccggcg tagtcaccgg tggttgcagc ggggttataa ccgagcaaaa agagtgcggc
    13981 aataattgcg ccattgacgc ccgtgccgcc aaatgcctgg gcggcgttat agcccaccag
    14041 aatcaccagg aaagtgaaca aacctttgct gaacaccttc ataaaattca gcgcgtcggg
    14101 tagcgtgccc tgagcatctg ccggaacgtg catcaccgtg gcaattaacg tcgctatccc
    14161 cagcagcaga ccggcggcaa taaaaccggg gatcagcggc gtgaagatgg tggcgaattt
    14221 ggcgagaaat tgttgcacgc cggaggtttg tttggctttt aactgacgtt tgttctgggc
    14281 ggcgatttct gcggcatcct gtaccggtgc ctcgcccagc agctcgctca tcgcttttgc
    14341 ggcacgatgc gctttaccag gcccaaaaac gacctgcacc tggtcgctgg tcaaaatgac
    14401 gcccttcacg ccttccagcg ttttgatatt gggatcaacc agtgaactgt catgtacacc
    14461 cagacgcagg tgcgtcatac agttaccaca actggcgata tttcccagtc cgccgacacg
    14521 ggtaagaatg gtgttcagaa gttcactgct gatctctttg gccatgggtt attccttgtc
    14581 taaaacctga cgaataaagc cgccgtgttg atccaggcgt tttttagctt ctgcggcatc
    14641 gagatttttc agcaccatca caatggccgt tttacagttg cgctcgcagg caattaacgc
    14701 cgcttccgct tgttcggcat tacatccggt ggcgttttta acgatattga cctggcgcac
    14761 atgcagtttt tcgttggtgg cgaccacatc gaccatcagg ttgccgaaca ctttgccgga
    14821 tttaatcatc agcccggtgg aaagcatatt gagcaccagt ttctgcgctg tgcctgcttt
    14881 cattcgcgaa gaaccagtga ccacttcggc acctacaatc ggtgtaatgg caaactcggc
    14941 ggtggtggaa acggcactcc ccggattaca ggaaattccc actgtgcggc aaccgagctg
    15001 gcgggcgtat tccagtccgg caatcacata cggcgtacga ccgctggcgg caataccgac
    15061 caccacatcc tgtgccgtta aatttatatt tttcagatcg ttaatgccgc cttctcggct
    15121 atcttccgtg ccttccaccg cgtgctgaat ggcatattcg ccgccagcaa tcaaaccaac
    15181 caccagaccc ggtttcacgc cgtaggtggg cggacattcg ctggcatcca gaatccccag
    15241 acgaccggat gttcccgcac cgagataaat cagacgaccg ccgccgctta cctgggcgtg
    15301 gataacatcg atcgccgcgg cgatatccgg caggacgcgc tcaacggcaa gcggtacggt
    15361 tttatcttca tcgttgataa tccggcacat ttccagcgtc gatacgcggt caatttcagc
    15421 cgaggcggtg ttcgagcctt cagtaatcat cttttcaagt tgcatcggtg gtgaccttac
    15481 tatttacgat caaataattg cagatgtatg gaatcatata tcccgaattg acagacttaa
    15541 aggaatcatt gagtctacac ttgcagagtg tgaagagcgt cataagaact taagcggaat
    15601 ttttgctgcc agcccgggag agtggaagat gttgtacctg acgaaaatac gcaatgcgga
    15661 aagtgaattt accgagaacg agcaaaaaat tgctgacttt ctgcgggcca acgtgagtga
    15721 gctgaaatcc gtctcttcgc gcaagatggc gaaacagctc ggcatcagtc agtcgagcat
    15781 tgtgaaattt gcccagaagc tcggcgcgca aggatttact gaattgcgta tggcgttaat
    15841 tggcgaatac agtgccagcc gggagaaaac gaatgccacg gcgctgcacc tgcacagttc
    15901 gattaccagt gatgattcgc tggaagtgat tgcccgcaaa ctgaatcgcg aaaaagagct
    15961 ggcgctggag caaacctgtg cgttgttcga ctacgctcgg ctgcaaaaaa tcattgaggt
    16021 catcagtaaa gcaccgttta tccagataac gggcctgggc gggtcggcgc tggtggggcg
    16081 cgatctgtcg ttcaaactga tgaaaattgg ctatcgcgtg gcctatgaag ccgacacaca
    16141 cgtacaagcc accgtttccc aggcgttgaa aaaaggcgat gtacaaatcg ctatctctta
    16201 cagcggcagt aaaaaagaga ttgtcttgtg cgcagaagcg gcacgaaagc agggggcaac
    16261 ggtgattgcc attacttctc tggctgactc tccgctgcgg cggctggcgc atttcacgct
    16321 cgataccgtt tccggtgaaa ccgaatggcg tagctcgtcg atgtctaccc gcacggcgca
    16381 aaattctgtt accgatttac tgtttgttgg tcttgtgcaa ctcaatgatg tggagtcatt
    16441 gaaaatgatt cagcgcagca gtgagctaac acaacgcctg aaatgatgca taaagcagcg
    16501 actggattga gattttcctg aattagtgag ctgatccgca gcaatatttt gtttatcctg
    16561 tattttcaga gggaatggag tgtaacgctc tgtattaaca aggagagcat taaaatgggt
    16621 aaactcacgg gcaagacagc actgattacg ggcgcattgc agggaattgg cgaaggaatt
    16681 gccagaactt ttgcgcgtca tggcgcaaac ctaatcttgc tggatatctc ccctgagatc
    16741 gaaaagctgg cggacgaact gtgtggtcgt ggtcatcgct gtacggcggt tgtcgccgat
    16801 gtgcgtgacc cggcgtcggt agccgcagct atcaaacgcg cgaaggaaaa agaagggcgc
    16861 attgatatcc tggtgaataa cgcaggcgtt tgtcgtctgg gcagtttcct cgatatgagc
    16921 gatgacgatc gcgatttcca tattgacatc aatattaaag gcgtatggaa cgtcacgaag
    16981 gcggtgctgc cggagatgat tgcccgcaaa gatggtcgca ttgtgatgat gtcttcagtc
    17041 actggtgata tggtggccga tcctggcgaa acggcgtacg ccttaacgaa agcggcgatt
    17101 gttggcctga caaaatcgct ggcggtggag tacgcgcagt ctggtattcg cgttaacgcc
    17161 atttgcccgg gatacgtgcg cacaccaatg gcggaaagca ttgcccgcca gtcgaacccg
    17221 gaagatccag agtcggtgct gactgaaatg gcgaaagcaa tcccgctgcg tcgcctcgcc
    17281 gatccgctgg aagtcggcga actggcggcc ttcctcgcat cggatgaatc cagctattta
    17341 accggtacac agaatgtgat tgatggcggc agcacactgc cggagacggt tagcgtcggt
    17401 atctgattca cctctgtttc ctccctgcat ttgtggggag gatttcgttt taaacccggt
    17461 tcttattccc ttttcaactt ccaaatcacc aaacggtata taaaaccgtt actcctttca
    17521 cgtccgttat aaatatgatg gctattagaa agtcattaaa tttataaggg tgcgcaatgg
    17581 ccgttaactt actgaaaaag aactcactcg cgctggtcgc ttctctgctg ctggcgggcc
    17641 atgtacaggc aacggaactg ctgaacagtt cttatgacgt ctcccgcgag ctgtttgccg
    17701 ccctgaaccc gccgtttgag caacaatgga ccaaagaaaa cggcggcgac aaactgacaa
    17761 taaaacaatc tcatgccggg tcatcaaaac aggcgctggc aattttgcag ggcttaaaag
    17821 cagacgttgt cacttataac caggtgactg acgtacaaat cctgcatgac aaaggcaagc
    17881 tgatcccggc ggactggcaa acgcgcctgc caaataatag ctcgccattc tactccacca
    17941 tgggcttcct ggtgcgtaag ggcaacccga agaatatcca cgactggaac gacctggtgc
    18001 gctccgatgt gaagctgatt ttcccgaacc cgaaaacgtc gggtaacgcg cgttacacct
    18061 atctggcggc atggggcgca gcggataaag ctgacggtgg cgataaagcc aaaaccgaac
    18121 agtttatgac ccagttcctg aaaaacgttg aagtgttcga tactggcggt cgtggcgcga
    18181 ccaccacttt tgccgagcgc ggcctgggcg atgtgctgat cagcttcgag tcggaagtga
    18241 acaacatccg taaacagtat gaagcgcagg gttttgaagt ggtgattccg aaaaccaaca
    18301 ttctggcgga attcccggtg gcgtgggttg ataaaaacgt gcaggccaac ggtacggaaa
    18361 aagccgccaa agcctacctt aactggctct acagcccgca ggcgcaaacc atcatcaccg
    18421 actattacta ccgcgtgaat aacccggaag tcatggataa actgaaagac aaattcccgc
    18481 agaccgagct gttccgcgtg gaagacaaat ttggctcctg gccggaagtg atgaaaaccc
    18541 acttcaccag cggcggcgag ttagacaagc tgttagcggc ggggcgtaat taatgtttgc
    18601 agtttcatca agacgcgtgc tgccgggctt taccttaagc ctcggcacca gtctgctgtt
    18661 tgtgtgcctg attttgctgc tgccgctctc cgcgctggtg atgcagctgg ctcagatgag
    18721 ctgggcgcag tactgggagg tgatcaccaa cccgcaggta gtagcggcct acaaagtaac
    18781 gctgctgtca gcgtttgtgg catcgatttt taacggcgtt tttggcctgc tgatggcgtg
    18841 gatcttaacc cgctatcgct ttccgggccg cacgctgctt gatgcgctga tggatttacc
    18901 ctttgcgctg ccaacggcag tcgccggttt aacgctagcc tcgctctttt ctgtaaacgg
    18961 tttttacggt gaatggctgg cgaagtttga tatcaaagtc acctatacct ggctggggat
    19021 tgcggtggcg atggccttta ccagcattcc gtttgtggta cgtaccgtgc agccggtgct
    19081 ggaagagtta ggcccggaat atgaagaagc ggcagaaacg cttggcgcaa cgcgctggca
    19141 gagtttctgc aaagtggtgc tgccggaact ttctccggcg ctggtggcgg gcgtggcgct
    19201 gtcgtttacc cgtagtcttg gtgaatttgg cgcggtgatt tttatcgccg gaaatatcgc
    19261 gtggaagacg gaagtgacgt cgctgatgat ttttgtgcgc ttacaggagt ttgattaccc
    19321 ggcagcgagc gcgattgctt cggtgatcct cgcggcatct ctgctgctgc tgttctcaat
    19381 taacactctg caaagtcgct ttggtcggcg tgtggtaggt cattaatggc ggaagttacc
    19441 caattgaagc gttatgacgc gcgcccgatt aactggggca aatggtttct gattggcatc
    19501 gggatgctgg tatcggcgtt catcctgctg gtgccgatga tttatatctt cgtacaggcg
    19561 ttcagcaaag ggctgatgcc ggttttacag aatctggccg atccggacat gctgcacgcc
    19621 atctggctga cggtgatgat cgcgctgatt gccgtaccgg taaacctggt gttcggcatt
    19681 ctgctggcct ggctggtgac gcgctttaac ttccctggac gccagttact gctgacgcta
    19741 ctggacattc cgtttgccgt atcgccggtg gttgccggtc tggtgtatct gctgttctac
    19801 ggctctaacg gcccgctggg cggctggctg gatgaacata atctgcaaat tatgttctcc
    19861 tggcccggaa tggtgctggt caccatcttt gtgacgtgtc cgtttgtggt gcgcgagctg
    19921 gtgccggtga tgttaagcca gggcagccag gaagacgaag cggcgatttt gcttggcgcg
    19981 tctggctggc agatgtttcg ccgcgtcaca ttgccgaaca ttcgctgggc gctgctttat
    20041 ggcgtggtgt tgaccaacgc ccgcgcaatt ggcgagtttg gcgcggtgtc ggtggtttcc
    20101 ggctcgattc gcggcgaaac cctgtcgctg ccgttacaga ttgaattgct ggagcaggac
    20161 tacaacaccg tcggctcctt taccgctgcg gcactgttaa cgctgatggc gattatcacc
    20221 ctgtttttaa aaagtatgtt gcagtggcgc ctggagaatc aggaaaaacg cgcacagcag
    20281 gaggaacatc atgagcattg agattgccaa tattaagaag tcgtttggtc gcacccaggt
    20341 gctgaacgat atctcactgg atattccttc aggtcagatg gtcgcgttgc tggggccgtc
    20401 cggttccggg aaaaccacgc tgctgcgcat tatcgccggg ctggagcatc aaaccagcgg
    20461 gcatattcgc ttccacggca ccgacgtcag ccgcctgcac gcgcgcgatc gcaaggtcgg
    20521 tttcgtgttc cagcattacg cgctgttccg ccatatgacg gtattcgaca atatcgcttt
    20581 tggcctgacg gtgctgccgc gtcgcgatcg cccgaatgcc gcagccatca aagcgaaagt
    20641 gacaaaattg ctggagatgg tgcagcttgc tcatctggcg gatcgttatc cggcacagct
    20701 ttccggcggc cagaaacagc gcgtggcgct ggcgcgcgcg ctggctgtag aaccgcaaat
    20761 tctgctgctt gatgaaccgt ttggcgcgct ggatgcgcag gtgcgtaaag agctgcgtcg
    20821 ctggctgcgt caactgcatg aagagctgaa attcaccagc gtgttcgtga cccacgacca
    20881 ggaagaagcg acggaagtag ctgatcgtgt agtggtgatg agccagggca atattgaaca
    20941 ggctgatgcg ccggagcagg tatggcgcga accagcgacc cgttttgtgc tcgaatttat
    21001 gggcgaagtg aaccgcctgc agggaaccat tcgcggcggg cagttccatg ttggcgcaca
    21061 tcgctggccg ctgggctaca cacctgctta tcaggggccg gtggatctct tcctgcgccc
    21121 gtgggaagtg gatatcagcc gccgtaccag tctcgattcg ccgctgccgg tacaggtact
    21181 ggaagccagc ccgaaaggtc actacaccca attagtggtg cagccgctgg ggtggtacaa
    21241 cgaaccgctg acggtcgtga tgcatggcga cgatgccccg cagcgtggcg agcgtttatt
    21301 cgttggtctg caacatgcgc ggctgtataa cggcgacgag cgtatcgaaa cccgcgatga
    21361 ggaacttgct ctcgcacaaa gcgcctgata ggttgagtga atgttaaacg cccggaggcg
    21421 cttcccgcga tccgggcttt ttaatggcaa ggtttgtaac ctgtagacct gataagacgc
    21481 gcaagcgtcg catcaggcaa caccacgtat ggatagagat cgtgagtaca ttagaacaaa
    21541 caataggcaa tacgcctctg gtgaagttgc agcgaatggg gccggataac ggcagtgaag
    21601 tgtggttaaa actggaaggc aataacccgg caggttcggt gaaagatcgt gcggcacttt
    21661 cgatgatcgt cgaggcggaa aagcgcgggg aaattaaacc gggtgatgtc ttaatcgaag
    21721 ccaccagtgg taacaccggc attgcgctgg caatgattgc cgcgctgaaa ggctatcgca
    21781 tgaaattgct gatgcccgac aacatgagcc aggaacgccg tgcggcgatg cgtgcttatg
    21841 gtgcggaact gattcttgtc accaaagagc agggcatgga aggtgcgcgc gatctggcgc
    21901 tggagatggc gaatcgtggc gaaggaaagc tgctcgatca gttcaataat cccgataacc
    21961 cttacgccca ttacaccact accgggccgg aaatctggca gcaaaccggc gggcgcatta
    22021 cccattttgt ctccagcatg ggaacaaccg gcactatcac cggcgtttca cgctttatgc
    22081 gcgaacaatc caaaccagta acgattgttg gcctgcagcc ggaagaaggc agcagtattc
    22141 ccggcattcg ccgctggcct gcggaatatc tgccggggat tttcaacgct tctctggtgg
    22201 atgaggtgct ggatattcat cagcgcgatg cagaaaacac catgcgcgaa ctggcagtgc
    22261 gggaaggaat attctgtggc gtcagctccg gcggcgcggt tgccggagca ctgcgggtgg
    22321 caaaagctaa ccctggtgcg gtggtggtag cgatcatctg cgatcgtggc gatcgctacc
    22381 tttccaccgg ggtgtttggg gaagagcatt ttagtcaggg ggcggggatt taagattatt
    22441 gcgcaggcac cgttttatcc gtctgcgcaa ttaaggcatg taaaaaaggc tgtgcgttct
    22501 catcgctctt accggggatc ttcacaatat acggcttccc ggtaatcgat gacgacgaag
    22561 caaccttatc aataaactgc tcggcagtgt caatgcggtt acgggtattg ccgagcttca
    22621 gacgcagatg agaaaccgct tcatcgcagg tatgttcatc accgttgcgc acaaatatca
    22681 aatccttttt ctgtgctaat ccctccagca tggcgttgat gcgggcttct tcatgaacag
    22741 ttaacttcgc gtaagcgggg agtgtcatta gtagtgtaat gaccagacaa atgattttct
    22801 tcattgaatt accttatcct tgttttgtga tagccgattt gactgccaga atcaggtaaa
    22861 gtttcggttg gaatacgatg aggatcatcg gcaaggagaa aaaattaaat gagtagtttg
    22921 ttgttgttta acgataagag tagggcgctg caggcggata tcgtcgccgt gcagtcgcag
    22981 gtagtgtacg gcagcgtagg caacagcatt gcggtgcctg ctataaaaca gaacggcttg
    23041 aatgtctttg ccgtgccgac ggtattgctg agcaatacgc cgcattatga cactttctac
    23101 ggtggtgcga ttccggacga atggtttagc ggctatttgc gtgcgcttca ggagcgtgat
    23161 gcgctgcgcc aacttcgtgc tgtaaccacg ggctatatgg gaacggcatc gcaaatcaaa
    23221 atccttgccg agtggctgac tgcgctacgc aaagaccatc ctgacctatt gatcatggtc
    23281 gatccggtga ttggcgatat tgatagcgga atttatgtca aacctgacct tcccgaggcg
    23341 tatcgacaat atttactgcc gctggcgcaa ggaattaccc ccaatatctt tgagctggaa
    23401 atcctgaccg gtaaaaattg ccgcgatctc gacagtgcca ttgctgccgc aaaaagtctg
    23461 ctttcagaca cattaaaatg ggtggtgatt accagcgcct ccggtaatga agaaaatcag
    23521 gagatgcagg tggtggtggt cagtgccgac agcgtgaatg tcatttccca ttcacgggta
    23581 aaaaccgacc tgaaagggac tggcgacctg ttttgtgctc agcttatcag tggtttgctg
    23641 aaagggaagg cgttaaacga tgcggtgcac cgtgcggggt tgcgcgtact ggaagtgatg
    23701 cgctataccc agcagcatga gagcgatgaa ttgattttgc cgccgctggc ggaagcataa
    23761 aaaatggcgc cgatgg
//
LOCUS       DAJVRF010000056        23378 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJVRF010000056 DAJVRF010000000
VERSION     DAJVRF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23378
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.056"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(269..886)
                     /gene="tdk"
                     /locus_tag="OQN13_002011"
     CDS             complement(269..886)
                     /gene="tdk"
                     /locus_tag="OQN13_002011"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCS1615375.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1491..1904
                     /gene="hns"
                     /locus_tag="OQN13_002012"
     CDS             1491..1904
                     /gene="hns"
                     /locus_tag="OQN13_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCS1615376.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2048..2956)
                     /gene="galU"
                     /locus_tag="OQN13_002013"
     CDS             complement(2048..2956)
                     /gene="galU"
                     /locus_tag="OQN13_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCS1615377.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="OQN13_002014"
     CDS             complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="OQN13_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCS1615378.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="OQN13_002015"
     CDS             complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="OQN13_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCS1615379.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5281..5739
                     /locus_tag="OQN13_002016"
     CDS             5281..5739
                     /locus_tag="OQN13_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCS1615380.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5789..6631
                     /gene="purU"
                     /locus_tag="OQN13_002017"
     CDS             5789..6631
                     /gene="purU"
                     /locus_tag="OQN13_002017"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCS1615381.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6791..6875
                     /locus_tag="OQN13_002018"
     tRNA            6791..6875
                     /locus_tag="OQN13_002018"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6825..6827,aa:Tyr,seq:gta)
     gene            6923..7054
                     /locus_tag="OQN13_002019"
     ncRNA           6923..7054
                     /ncRNA_class="other"
                     /locus_tag="OQN13_002019"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7238..7915)
                     /gene="narI"
                     /locus_tag="OQN13_002020"
     CDS             complement(7238..7915)
                     /gene="narI"
                     /locus_tag="OQN13_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCS1615382.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7915..8625)
                     /gene="narJ"
                     /locus_tag="OQN13_002021"
     CDS             complement(7915..8625)
                     /gene="narJ"
                     /locus_tag="OQN13_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCS1615383.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8622..10160)
                     /gene="narH"
                     /locus_tag="OQN13_002022"
     CDS             complement(8622..10160)
                     /gene="narH"
                     /locus_tag="OQN13_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCS1615384.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10157..13900)
                     /locus_tag="OQN13_002023"
     CDS             complement(10157..13900)
                     /locus_tag="OQN13_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCS1615385.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14293..15684)
                     /gene="narK"
                     /locus_tag="OQN13_002024"
     CDS             complement(14293..15684)
                     /gene="narK"
                     /locus_tag="OQN13_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCS1615386.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16023..17819
                     /gene="narX"
                     /locus_tag="OQN13_002025"
     CDS             16023..17819
                     /gene="narX"
                     /locus_tag="OQN13_002025"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCS1615387.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17812..18462
                     /gene="narL"
                     /locus_tag="OQN13_002026"
     CDS             17812..18462
                     /gene="narL"
                     /locus_tag="OQN13_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCS1615388.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18463..19857)
                     /gene="ychO"
                     /locus_tag="OQN13_002027"
     CDS             complement(18463..19857)
                     /gene="ychO"
                     /locus_tag="OQN13_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCS1615389.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20042..20395
                     /gene="ychN"
                     /locus_tag="OQN13_002028"
     CDS             20042..20395
                     /gene="ychN"
                     /locus_tag="OQN13_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCS1615390.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20439..21134)
                     /gene="chaC"
                     /locus_tag="OQN13_002029"
     CDS             complement(20439..21134)
                     /gene="chaC"
                     /locus_tag="OQN13_002029"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCS1615391.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21292..21522)
                     /gene="chaB"
                     /locus_tag="OQN13_002030"
     CDS             complement(21292..21522)
                     /gene="chaB"
                     /locus_tag="OQN13_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCS1615392.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21792..22892
                     /gene="chaA"
                     /locus_tag="OQN13_002031"
     CDS             21792..22892
                     /gene="chaA"
                     /locus_tag="OQN13_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCS1615393.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23295..>23378
                     /locus_tag="OQN13_002032"
     CDS             23295..>23378
                     /locus_tag="OQN13_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCS1615394.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIV"
BASE COUNT         5860 a         6116 c         5626 g         5776 t
ORIGIN      
        1 ttctggggcc gtttttttgt ttacccaaag caacttttcc ataaaccgac agcattagcc
       61 ttcatcatat ttgcgacgat gtataacgcc taaacacagg gatattgtac tttacaggtc
      121 acaagtcaac gtcggtgctt aagagccctg tgaggcgtat agcggcgtta aaaaactgcc
      181 gagaagggta tatagcccgg aagaagtgcg taaaacgaac tgacaggata aaagtgccct
      241 gctcaccctg tcagtaaaga aattcttatt aatcgtggcg atgcctttcc tgaatagccg
      301 ttaatgagcc gacttgtaac gcctctttat agtgtttacg gcatacagaa acgtatcgtt
      361 cattaccacc aattaccacc tgctcacctt cgttataagg tctgcctgct tgatcaagac
      421 gcagcaccat gcttgcttta cggccacaaa aacagatggt ttttaattca accagtttgt
      481 cggaccatgc cagtaagtat tggctgccaa taaataattc acctcgaaaa tcggtacgta
      541 aaccataaca aagtacgggt atatcgagtt gatcgacaac ctccgataat tcatatactt
      601 gttgtctggt taaaaactgg cattcatcaa ccagtacgca atgaattgcc tgctgttcat
      661 gttccgcacg aatctcatca aataatgatg aattttggtt aaataatttt gcaggcgatg
      721 acaaacctat acgcgaactg actttcccgg caccaaagcg atcatcaatt tctgccgtat
      781 atacgacagt gcgcatgccg cgttcctggt aattgtatga agattgcaac aatgctgtag
      841 acttacccgc attcattgcg gaatagtaga aatatagctg tgccatcagc cacaggccct
      901 caatgatatg aaataaagtt ggctggagtt tatcataatt cgtcagtttt tcagctcaac
      961 agtatgcaca gaagtgggta tatgcgttct cccttacgaa gccttgcata atccttctga
     1021 gctatcatta caactgcctc gcttgttata agcgggtaaa tgactactgg taactattca
     1081 caatctttaa cctgttgcgc aagtaatagc cctctgttga cctccaggag atagtgcaat
     1141 actaagtcca tgctcttatt gcgactgttc tacttttcat cattcgctta atagggaatt
     1201 ctcgtaaaca caactaatac agaagactga aaggtcgtca gcctacgata atctccccat
     1261 aaaatgtgac atgaatcagg aagttttaac ctcacgtgct gcgaaatcat cggtgtaaat
     1321 agggctatat gccgcgtctt ttctggctaa ttttatgaaa agatatttat tggcggcaca
     1381 aaataaagaa caattttgaa ttccttacat tcctggctat tgcacaactg aatttaaggc
     1441 tctattatta cctcaacaaa ccaccccaat ataagtttga gattactaca atgagcgaag
     1501 cacttaaaat tctgaacaac atccgtactc ttcgtgcgca ggcaagagaa tgtacacttg
     1561 aaacgctgga agaaatgctg gaaaaattag aagttgttgt taacgaacgt cgcgaagaag
     1621 aaagcgcggc tgctgctgaa gttgaagagc gcactcgtaa gctgcagcaa tatcgcgaaa
     1681 tgctgatcgc tgacggtatt gacccgaacg aactgctgaa tagccttgct gccgttaaat
     1741 ctggcaccaa agctaaacgt gctcagcgtc cggcaaaata tagctacgtt gacgaaaacg
     1801 gcgaaactaa aacctggact ggccaaggcc gtactccagc tgtaatcaaa aaagcaatgg
     1861 atgagcaagg taaatccctc gacgatttcc tgatcaagca ataatctttt gtagattgca
     1921 cttgcttaaa atcccgccag cggcgggatt ttttattgtc cggtttaaga caatttaata
     1981 agaataagct ataaaaaaac ggcgtcgatt gctcaacgcc gtttcgtgga taacaccgat
     2041 acgaatgtta cttcttaatg cccatctctt cttcaagcca ggctttaaat tccgtgccaa
     2101 gggtgttatg acgaataccg tattcaacga aggcctgcat gtaacctaat ttattaccgc
     2161 agtcatggct cttccctttc atatgatagg cttccaccgt ttctttttcg atcagcatat
     2221 caattgcgtc ggtgagctga atttcatcac cagctcccgg aggggttttt gccagcaacg
     2281 gccaaatatc cgcgctaagt acgtaacgac ccacaatagc gagattagac ggcgcaacat
     2341 ccgctttcgg tttttctacc acaccaacca tcggtacgct ttcacccggc gctaattcaa
     2401 cgcctttgca atccacaacg ccatatgcgg tcacatcagc aaccggttca accatgatct
     2461 ggctatgacc cgtttcatca aagcggcgga tcatctctgc caggttatcc tgtgacaaat
     2521 cggattcata ttcatccaga ataacatcag gcaaaataac agctaccggt tcatcaccca
     2581 ctaccgggtg agcacacaat accgcgtgtc ccaggccttt cgccagaccc tgacgaactt
     2641 gcataatagt cacgtgcggt ggacaaatag actgcacttc atcaagcagt tgacgtttta
     2701 cacgtttttc cagcattgct tccagttcaa aactggtatc aaagtggttt tcaatagagt
     2761 ttttagatga gtgtgtaacc agcacaattt cagtaatgcc agccgcaata cattcattca
     2821 cgacgtattg aattaatggc ttatcgacaa gtggcagcat ctctttcggg atggctttcg
     2881 tcgccggcaa catcctggtt cctaatcccg caacggggat aacggctttt ttgactttcg
     2941 tattaatggc agccatttaa attctcctgg actgttcgtg ttttgaacgt gttcatattt
     3001 ctgtatcgca tcccagtata tcagcacccc gaaggggcta agttctgaaa aggaggcgag
     3061 ttagcataat taagacaatt acttatacga atggcgacga tagtaccacc agccgacatt
     3121 agcaggtaat gcaaatttag cccgcgttat cgtttgctca ttctgcagac aacatcaagc
     3181 gcagtcgacc accggttccc catatttggc attgccaggc atcgcatcgc tggctcaatt
     3241 gattcaaata agcgttacct aaagtgccta acggaacacc attactgatt tgcacctggt
     3301 gttcgccggt atttaacgtc gcattcagac ccgcagaaac cagaatgaga tttttcagtt
     3361 cgcgatgata atagccaact aatagcggaa actgccccgg cagattggcc tgacggagta
     3421 aatggtttac ctgcttcaat aacgcgccca actctggcaa ccgttgattt tggtgtgcaa
     3481 gctgttcctg taataatccg ttaaacaatg cgcgtaataa caaggcagca agtacgccat
     3541 tatgtccagc tcgggtgaca tcaaggcaat aaaatgccag atcgttttcc gaaagtgcgg
     3601 caatatcaag caccaggccg ggtttgtccg cggcaaccaa ttgacgataa ttaacccggc
     3661 aatgggaaat cacctgctga accggcggtt gtagttcctg taataatttc gccgctgcgg
     3721 cagggttatc aaccattgca tcccagtcgc gaaaaagcct ttcctcttcc tcaacgcgcg
     3781 aattaaacat gctgggatag agacaggcaa aaaccatctc gcgcaagcga ttcagatctt
     3841 taactggttt cagcaaaaca tcttcaacgc ccagacgtaa cgctttggca atatctgcca
     3901 tattttcagt ggcagatatc accagaactg gggtctggtc gcctctgtta cgtatatgct
     3961 ccagcagttt aagcccgttc attcgtggca tcgcgatatc acatatcatc aggtctggag
     4021 tgaaacctcc cagcaactca agggcatcca ccccatcagc cgccagtacc gttgtcgctc
     4081 ccaatgagga aaaccatgaa tccagaagcg agcgaaatac ctgctcatct tcaacaatga
     4141 gaatctgttt tccgaccaat ggctgcgtca tgttctctcc cctgactggc tttactcaat
     4201 agtggcatgc tattgccacc tgcgcctgtc agaattaact taagtgtaga taaaaattct
     4261 ggtcaaatgt tggtgcgtac caacggcaaa agttcgtcca ttttcctttc cactgccagc
     4321 tgtccggccg caatggcagc gtgcgcgcga tggaaatcaa gcgtagatat ttgcgggcaa
     4381 acaggttgaa tcagaatatc gggcggatca cctgccatgc ggttcctttt aaggcggttc
     4441 tccaacacct ggatagacgt ggtcataatc tctgttgccg ttggcgctgt cacagcccga
     4501 cgtgtcgtta tgctgcctaa cctttctttc agacgcgcat gccacggtag agaatcacca
     4561 ttctcgctat tttcttcact gacattaaag gagagcagat cttgttgcat caaatgagca
     4621 tcgtgctgca ggtcgaccgc tatcacaata tcagccccca atgcacgcgt aagagaaata
     4681 ggaattgggt taactactgc cccatcaacc agccagtagc cgttatgtgc aacgggtgcc
     4741 atgagtcctg gaatactgca tgatgcgcga atagcaagat ggagatcgcc ttcagtaaac
     4801 cataattcac gccccgtact taaattggtg gcaacagccg caaagcgacg ggaacaattt
     4861 tcgatctctg tttccggcat tatttcgcga tattgattga agacacgctc gccgcgcagt
     4921 aacccaccgc gctgccagga gaggtccatc aggcgtaaaa cgtcccaata actgaaagag
     4981 gttacccaat cttccagcgc agataatcga tcgcatgcat aggccgcgcc caccagcgaa
     5041 ccaattgaac atcctgcaac gatatcaatt tcaataccca ctttttttag cgcattaata
     5101 acgccaatat gcgaccaacc tctcgccgcg ccagatccca gcgccagccc tatttttatc
     5161 tttctcatta ttcccgcaag attcccctgg aatgcaatcg tagccacatc gctactgctc
     5221 ggttaacata gtgctactca ggcggttaat acgccgttat tgtttccagg gagattattt
     5281 gtgtctcagc tttgtccctg tggtagtgct gtcgagtata gcctatgttg ccacccttat
     5341 gtgtctggtg aaaaggttgc acctgatcca gaacatctca tgcgttcgcg ttactgcgct
     5401 tttgtgatgc aagacgcaga ttatttaata aagacctggc atccctcttg tggagcagcg
     5461 gcgttacgtg ccgaattgat ggccggattt gcgcatactg aatggttggg attgaccgta
     5521 tttgaacatt gttggcagga cgccgacaat atcggatttg tcagttttgt tgctcgattt
     5581 accgaaggtg gcaaaacagg tgcaataatt gaacgttcgc gatttttgaa agaaaacggt
     5641 caatggtact atattgacgg tacacgtccg cagtttggtc gcaacgatcc ctgcccttgt
     5701 ggttcaggta aaaaatttaa aaagtgctgc ggccaataat ggttgacggt acggtttagc
     5761 aaacactctc aacaaggttt ttccagcaat gcattcactc caacgtaaag ttctgcgtac
     5821 tatttgtccg gaccaaaaag gtctgatcgc acgtattacc aatatttgct acaagcacga
     5881 gttaaatatc gtacagaaca atgaatttgt tgatcaccgt accgggcgct tttttatgcg
     5941 cacggaactg gaagggattt ttaatgattc caccctgctg gcggatctcg atagcgcatt
     6001 gccagaaggc tccgtgcgtg agctgaatcc tgccggtcgt cgccggatag tgattctggt
     6061 cactaaagaa gcgcattgcc ttggcgattt gttgatgaaa gccaattacg gcggcctgga
     6121 tgtcgaaatc gcggcagtta ttggtaacca cgatacttta cgttctctgg ttgagcgttt
     6181 tgatattccg tttgagctgg taagccatga agggttaagc cgcaacgagc acgatcaaaa
     6241 gatggcggaa gccattgatg cttatcaacc tgactatgtg gtgctggcga agtatatgcg
     6301 ggtattaacg ccggaatttg tggcacgctt cccgaataaa atcatcaata ttcaccattc
     6361 attcctgcct gcgtttatcg gcgcacgtcc ttatcaccag gcctatgaac gtggtgtgaa
     6421 gattattggc gcaaccgctc actatgtgaa tgacaatctg gacgaaggcc caatcatcat
     6481 gcaggacgtt attcatgtcg atcataccta cacagctgaa gatatgatgc gcgcaggtcg
     6541 tgacgtcgag aaaaacgtct taagtcgtgc gctatacaaa gtactggcac agcgcgtctt
     6601 tgtttacggt aatcggacga ttattcttta atcgccagca aaaataactg gttaccttta
     6661 atccgttacg gatgaaaatt acgcaaccag ttcatttttc tcaacgtaac actttacagc
     6721 ggcgcgtcat ttgatatgat gcgccccgct tcccgataag ggagcaggcc agtaaaaagc
     6781 attaccccgt ggtggggttc ccgagcggcc aaagggagca gactgtaaat ctgccgtcat
     6841 cgacttcgaa ggttcgaatc cttcccccac caccatcact tttcaaaagt ccgtaaactc
     6901 tcaaacgaat ccgcaattaa atattctgcc caagcgggga aggatgagaa gcttcgacca
     6961 aggttcgact tgagcgccag cgagagagcg ttgccgcagg caacgacccg aagggcgaag
     7021 cgcgcagcgc tgagtaatcc ttcccccacc accatcgctt ccttaaataa tcaaaacctg
     7081 ttactccgag ctaattttgc ccgatggcac cacgtttacc gggcctacaa tatcctcccc
     7141 tgtaggtcgg ataagcgaag cgcatccgac aactcacccc gcaccgtgcc cataaaaaaa
     7201 ccccgccgaa gcagggtcta tgtgaactaa aattcgctta gtgacgagcg cgcaccagct
     7261 ggtacttacg tgtcagatac tccaccggta cgctccagat gtgtaccaga cgcgagaacg
     7321 ggaacagcag gaataacgtc atccccagca ccaggtgcag acggaagata aacgccacac
     7381 catcgaggtg ttgagaagcg ccaccgtgga aggtcaccac cgactgcgcc cagccaacca
     7441 gtttcatcat ctcgctaccg tccatatgct gagcggagaa cggaatggtc aacaggccca
     7501 gcgcgcactg gataacgagc agcgacagga tcaggatatc cgctccggta gtggttgcac
     7561 gcacgcgtgg gctgaacaga cgacgcttca gcaacagcac gccgccaatc agacacagca
     7621 cgccgctggc accaccagca aacatcgcca ttttctgttt cacttcaatc ggcagccagg
     7681 cttcatacat ccagtgcggc gtcagcatac cgaagaagtg accaacaaaa atccccagaa
     7741 taccgatatg gaacaggttc gacgccaggt tcatcccttt gcgatccagc atctggctgg
     7801 acgccgcgcg ccaggtgtac tgcccgtagt cataacgcag ccagctacca atcaggaaga
     7861 ccgccccggc tatgtacggg tagatatcaa agaagaacat attcaggaat tgcattagtg
     7921 ctgtcctccg gtggtgatat tcagatattg cggcgcgacg gcaccggcaa agcgacgctg
     7981 atgagcagtg attgctgaat caccgcagcc tttgtcagca aagaatttaa cctgctcttc
     8041 ttcccagaca gcatccagcg cctgcggcgt atcatcgcgc gcttcgtcgg caattttttc
     8101 cgccactttg tcgctgtcga tagcggtatt ggccaatttc agcagcagat caaacatcac
     8161 ggcataacgg ctttcacgct gttgcagacg cgcgctcagc aatgccagaa tcggcgcgat
     8221 atctttcaaa ccttccacgg cttcgctttg cggcagctgc gacaggtact ccagatacag
     8281 cggcagatgg tccggtaatt cgcggctgtt taactgcaag ccgtgctgct cgtactgcgc
     8341 cagcaggtcc accatcgcct ggccgcggtc acgggattca ccgtgaacat gttcaaatag
     8401 caacagtgac gtggcgcgac cacggtcgaa cagttcgctg tactgggcct gggcatcgag
     8461 cggatccatc gccgttaaat cgcgcaggaa aatgcccagc gcatgggcat cttctttcga
     8521 cagatttttc gacgcagcaa tcgcctcaaa catctcttgt tgatgctgcc ataaggcagc
     8581 atccggatat tcaaggagac gcgatacaat cacgagttcg atcatggatg cggctccgtt
     8641 ttgctggtca catcgatggc atcgatacga cggctgttga acagattgaa tttggtatct
     8701 gaaccgtggc agccatcacc aaaggtaaag ccgcagccat ttttctccgg gaaggcttcc
     8761 cgtgccagtt cacgatgact actcggcacc acaaagcgat cttcgtagtt agcaatcgcc
     8821 agataacggt acatctcctg tgcctgggct tcggtcagac cgacctcttc cagcgcacgg
     8881 gtatcaactt taccgtcaac ggtttcagca cgtttgtaat gacgcatcgc cagcatacgt
     8941 ttcagtgcgc gcagtaccgg tttggtatca ccggcggtca gcagattcgc cagatactgt
     9001 accggaatac gcaagctttc gacgtctggc agaatgccgt tgctacccag ctcacccgcg
     9061 tctgctgcag actgaatcgg agacagaggc ggcacgtacc agaccatcgg cagtgtgcga
     9121 tattctggat gcagcggcag cgccagtttc cattccattg ccattttata aaccggcgac
     9181 tgctgtgcgg cttcaataac gctcagcgga ataccgtctt taatcgcctg ctcgatgact
     9241 ttcggatcgt tcggatcgag gaacacctcc agctgacgct ggtaaagatc tttctcgttc
     9301 tcggtgctgg ctgcacgttc aatcgcgtcg gcatcgtaca gcagcacgcc aagataacgg
     9361 atacgaccga cacaggtttc tgagcacacg gtcggctgac ccgcttcaat acgcggatag
     9421 cagaagatgc acttctcaga cttaccgctc ttccagttga agtagatttt tttgtacggg
     9481 catccggtga tgcacatacg ccagccacgg catttatcct ggtcgatcag gacgatgcca
     9541 tcttcttcac gcttgtaaat cgcaccgctc gggcaggtcg ccacacatgc cgggttcagg
     9601 cagtgttcgc acaggcgcgg caaatacatc ataaaggtgt tttcgaactg gctatacatc
     9661 gccttctgga tgttgtcgaa gttcttgtct ttcgccagtt tgtcaaactc accacccagg
     9721 tcatcttccc agttcggccc tttttcgatt ttcgccatcc gttccccggt aatcagcgaa
     9781 cgcggacggg caatcggctg cgatttgctg ccttccggcg cggtatgcag gttctgatag
     9841 tcaaaatcga acggctcgta ataatcgtcg atccccggca gatgcgggtt agcgaagatt
     9901 ttacccagca acatggcacg gttacccatg cgcggctgca gtttgccgtt gattttacgg
     9961 atccagccgc ctttgtattt ttcctggttt tcccagtcag tcgggaagcc ctgacccggc
    10021 ttggtttcca cgttgttgaa ccacgcgtat tccacgcctt cacggctggt ccagacgttt
    10081 ttacaggtaa ctgaacaggt gtggcagccg atgcacttat cgagattcag caccatgccg
    10141 acttgtgaac gaattttcat tttacgctct cctgtacctg gtcattgcct tcgccatcta
    10201 accagtcaat gttcttcatc ttacgcacta caacaaactc atcgcggtta gaacctacgg
    10261 tgccgtagta gttaaagccg tatgccagat gggcatagcc gccgatcata tgcgtcggtt
    10321 tcggcgtgat acgggtgacc gagttatgga taccaccacg ctgttgggta atttccgaac
    10381 caggcaggtt aacgatacgt tcctgcgcgt ggtacatcat ggtcatccct gccggaacac
    10441 gctggctgac aaccgcacgg gcagtcagag caccgttgct gttgaatact tcaatccagt
    10501 cgttatcggc gatacccaga tctttggcat cggcttcact caaccagacc accggaccac
    10561 cgcgacctaa agtcagcatc agcaggttgt cgctgtaggt ggagtggata ccccacttct
    10621 ggtgcggcgt caggaagttg agcgcttttt ccgggttgcc gttggatttc tggcctatca
    10681 cttctttcac cgaacgggtg tcgatcggcg gacgataaac cagcaggctt tcaccgaaat
    10741 cacgcatcca ctggtgatcc tgatacagtt gctgacgacc agagagcgta cgccatggga
    10801 tcagctcgtg aacgttggtg taaccggcgt tgtaagaaac gtgttcatct tccagaccag
    10861 accaggtcgg gctggagata attttgcgcg gctgtgcctg aatatcgagg aagcggatct
    10921 tctcgtcttc tttattcagc gccagatgcg tatggtcacg accggtaaat tcgctcaggg
    10981 cagcccaggc tttcacggct acctgaccgt tggtttccgg tgccagtgtc aggatcatct
    11041 ctgccgcatc aattgcggtg ttcagcatcg gctggccttt cgccggacct tccgctttgg
    11101 tgtagttgag cttacgcagc agatccatct cgctctgggt gttccaggca atccctttac
    11161 cgccattacc gattttctcc atcaacgggc caatagaggt aaagcgttcg taagtcgccg
    11221 gataatcgcg ctctacgacc ataatgtgtg gcgcggtttt acctgggatc aggtcgcact
    11281 cgcctttttt ccagtctttc acatccagcg gctgcgccag ttcagcggca gagtcatgct
    11341 ggataggcag cgtgacgatg tcggtttctt tacccagatg gccgacgcac acttcggaga
    11401 atttcttcgc gatggctttg tagatttccc agtcgctttt cgcttcccag gccggatcga
    11461 ccgccgcaga cagcgggtga ataaacggat gcatatccga agtattcatg tcgtcttttt
    11521 cgtaccaagt cgccgtcggc aaaatgatgt cggaatagag acaggtgctc gacagacgga
    11581 agtccagcgt aaccaccaga tccagcttgc cttccagacc attgtcctgc cagtccactt
    11641 cttccggctt cacgccgccc tgttgcccca gatctttacc ctggataccg tgctccgtcc
    11701 ccagcaggta cttgagcata aactcatgac ctttaccgga agaaccgagc aggttagaac
    11761 gccagatgaa caggttgcgc gggtggtttt taccgttttc tggttgttct gccgcaaaac
    11821 ggatggaacc ctctttcagg gatttcaccg tatagtccac cggattcatc ccggcttttt
    11881 ccgcttcgcc agcgatagtc agcgggttag tgcctaactg cggtgcagac ggcagccagc
    11941 ccatgcgttc cgcacggacg ttaaagtcga tcaagtgtcc ggtatagcgg gatttgtccg
    12001 ccatcggtga cagcaactct tccgccgtga cggtttcata acgccactgg ctggagtggt
    12061 tatagaaata agaagtgctg ttcatgtgac gcgccggacg ctgccagtca agggcaaacg
    12121 ccagcggctg ccagccggtt tgcggacgca gtttttcctg acctacatag tgcgcccagc
    12181 cgcccccgct ctgaccgaca cagccgcaga aaatcagcat gttgatcaga ccacgatagt
    12241 tcatatcgag gtgataccag tggttcagcc ccgcaccgac gataatcatc gaacgaccgt
    12301 gcgttttatc agcgttatcg gcaaattcac gggcgatgcg aataatctgg ctgcgagaaa
    12361 cgccggtaat ctgctcggcc caggccgggg tataagcttt cacatcgtca tagctggttg
    12421 cacagttaac gtcgttcagg ccacgttcca gaccgtagtt tgccagcgtc agatcataaa
    12481 cggtgctcac cagggcggtg ctgccatcag ccagttgcag gcgtttcacc ggcagtttgt
    12541 gcagcagcac gttttccagt tccactttgt tgaagtgttc cgtgccgtcg ccaccaaagt
    12601 acgggaagcc tacctcagcg atctcatcct ggctacccag caggctcagt tgcagctcgg
    12661 tttcttcgcc agttttgccg tcgcgctgct caagattcca tttgcccttc tcgccccagc
    12721 ggaagccaat agaaccgttc ggcgcaacca tttcgccatt ggtattaaag gcgacagttt
    12781 tccattccgg attgttttcc tggcccagcg catcaaccag atcagcagcg cgcagcatac
    12841 gacctgcagc gtagtaaccg tcgcgttctt ccagcatcac cagcatcggc atgtcggtgt
    12901 agcgacgcac atagtcggtg aaatactggc ttgggttgtc gaggtggaat tcacgcagca
    12961 ttacgtggcc catcgccagc gccattgccg catcggtgcc ctgtttcggt gccagccaca
    13021 gatcgcacag tttggcgatt tcagcgtagt ctggtgtgac ggcaacagtt ttggtgcctt
    13081 tgtaacgcac ttcagtaaag aagtgagcat ccggggtacg cgtctgcggc acgtttgaac
    13141 cccaggcgat gatgtagctg gagttatacc agtcagcaga ttccggtacg tcagtttgct
    13201 cgccccaggt ttgcggagac gcaggaggca ggtcgcagta ccagtcgtag aagcttaagc
    13261 aagtaccgcc aatcagcgag agatagcgtg cacccgatgc gtaagaaacc atcgacattg
    13321 ccggaattgg cgagaaacca gcaacacggt ccgggccgta gtttttgatg gtgtaaacgt
    13381 tagatgcggc gatcagttcg ttcacctcct gccaggaaga acgaacaaat ccaccgcgtc
    13441 cacgcgcctg cttaaagctt ttcgctttat cggcgtcttc aatgatagaa gcccatgcct
    13501 caaccggatc gctatgcagc gccttcgctt cacgccacat tttcatcagg cgtttgcgca
    13561 tcatcgggta tttcaggcgg ttggcactgt aaagatacca ggagtagctg gcaccgcgcg
    13621 ggcagccgcg aggttcatgg tttggcagat ccggacgggt acgcggatag tcagtctgct
    13681 gggtttccca ggtgaccaga ccgtttttga cgtagatttt ccagctgcag gagccggtgc
    13741 agtttacccc gtgggtagag cggacgattt tgtcatgctg ccaacgctgg cgatatccat
    13801 cctcccagtc acggttggta ttgagaagct ggccatgccc atcggcaaag gtttcaccct
    13861 tctgtttgaa gtagcgaaac cggtccagga atttactcat cggttttctc ctgtgggagc
    13921 ctgtcggccc tctgataaat cgacattgct aatgtttaag ggcaaggtaa cgctctgaaa
    13981 cagcgtggga attgataacg atcaagagtg atggggatga aaaataaagt aaattcctaa
    14041 gatacccctt taggataatt aacagccacc acataacaga ctgtaaatta agacgtttta
    14101 tacctgtagt tatttaaact attaagattt ttatgcagat gggtattaag gagtattccc
    14161 catgggtaac atattaatgg ctcttaatga aatccggcga aataagccat ataatatgag
    14221 tatgttgtaa ctaaatgacg cataaaaaaa gcgcgacatc atgccgcgca aaggataatc
    14281 aaatattaag atttattttt tagaatgccg accatatacc gcccaggtaa tcactacgca
    14341 ggcgatatag aaaatcaaaa ataccttcat tgcgccgact ggcgaacccg ttaatgccag
    14401 cgagctacca aacgctttcg ggataaagaa gccaccaata gcgccaatcg cagagatgaa
    14461 acccagcgcc gctgccgtgt cggttgccgc ttcacgcatc gcacgttcgt cagaaccccc
    14521 ttctgctttc acgcgatcca ttgtcagttt acggaagatc actgaaatca tctggaaagt
    14581 ggaaccacta cccagcccag ctgtcaggaa cagcgccagg aagaccgcga agaacgccat
    14641 gaagcttccg ccctgcccgt cagtcggtaa ggtcaggaac agcaggccgc tgaaaatcgc
    14701 catcagaata aagttcacca gcgtgacacg agttccgccc agacggtcag ataatgcacc
    14761 acctgcagaa cgcgccagcg caccaataaa cggcccgaag aaagcgtatt gcagaatctg
    14821 aacatccggg aactgcgttt ttgacagcat cgcaaagccc gcggagaagc cgataaagga
    14881 gccgaaggtt gccagataca gcaggctcat aatccacaga tgaccccgtt tgagtaccgg
    14941 caactgctcc ttgatggagg ctttcgaggt agcaagatcg ttcatgccaa accacgccgc
    15001 aatggtgaag atggcaagga acggcaccca tatccaggac gcattcgcca gatacagctc
    15061 agtcccatcc ggctgtttga cgccctggct accaaatact gcgaaaatcg acagtgatac
    15121 caccagcgga gcaaccaact gcatgacgct gacgcccatg tttcccagac caccattcag
    15181 acccagcgcg ccaccctgct tctgtttcgg aaagaagaag ctgatgtttg ccatactgga
    15241 tgcgaagttc gcgccagcaa agccgcatag cagagagatg atgatgaaga cgctataagg
    15301 cgtggaggta tcctgcacgg caaaacccag ccagacgcaa ggaataatca gaataccggt
    15361 gctgaacgcc gtccagcgac gaccaccgaa gataggaacc ataaaggagt atggaacacg
    15421 taataacgcg ccagaaaccg aaggcagcgc agtcaacata aatagctgat cggtcgtaaa
    15481 attaaaaccg actttcggta agttcaccgc aacagcgctg aacaacatcc atacgcaaaa
    15541 cgccagcagc agacagggaa cggaaatcca caggttacgg ctggcaatac gttgaccgcg
    15601 ttgttgccag aacgcaggat cttccggtcg ccaatctgta atgacagctc cagtagccct
    15661 ttcgggggcg gatgagtgac tcatagacac ctctgatact cgtttcgatg tctgccacct
    15721 tagtgtctgt agctaaaggc aatttgatgt aaatcaaacg ataagataac tttatctatc
    15781 attgatattt atcattaccc atagtgagta cagtgatttc ataaaaatta tgagattttt
    15841 cacggtgctg taaaatccct acccttaccg atgtaaagcg actaaccaca cggcaaataa
    15901 ggagtaactc tttccgggta tgggtatact tcagccaata gccgagaata ctgccattcc
    15961 agaatgtatc gtcacattca ttaaggttat tgctcattta aagcctgaag gaagaggttt
    16021 gcatgcttaa acgttgtctc tctccgctca ccctggttaa tcaggttgcg cttattgtgt
    16081 tgctttctac tgctattgga ctggcaggga tggcggtttc tggctggctg gtgcaaggcg
    16141 ttcagggcag cgcccatgcg atcaacaaag cgggatcgct gcgcatgcaa agttaccgtc
    16201 tgttggcggc agtgccatta agtgagaaag acaagccctt aattaaagag atggaacaaa
    16261 cggcatttag cgccgagtta actcgagcag cagaacgaga cgaacaactg gcgcaattac
    16321 agggtttaca agattactgg cgtaatgaac tgatccctgc actgatgcgt gcacaaaacc
    16381 gcgaaacggt gtcagcggat gtcagccagt ttgttgccgg gcttgatcag ctggtatctg
    16441 gttttgaccg caccacggaa atgcgcattg agacagtggt actggtccat cgggtaatgg
    16501 cggtatttat ggcactttta ctggtgttca ctattatctg gttgcgggcg cgactgctac
    16561 aaccgtggcg gcaactgctg gcaatggcga gtgccgtcag tcatcgcgat tttacccaac
    16621 gcgcgaacat cagcgggcgc aacgaaatgg cgatgcttgg aactgcattg aacaatatgt
    16681 ctgcagaact ggccgaaagt tatgccgtac ttgagcagcg ggttcaggag aaaaccgccg
    16741 ggctggagca taaaaatcag atcctctctt ttttatggca ggctaaccgc cgtttgcatt
    16801 cccgcgcccc gctgtgtgaa cgcctgtcac ctgtactcaa cggcttacag aatttaaccc
    16861 tgctacgtga tatcgaattg cgggtgtatg acactgatga tgaagagaat catcaggagt
    16921 ttacctgcca gccagatatg acttgtgatg ataaaggctg ccagctctgc ccgcgcggcg
    16981 tattacccgt tggtgatcgc ggcacgaccc tgaagtggcg gctggctgac tctcatacgc
    17041 agtacggtat tttgctggcg accctgccgc aggggcgtca tcttagccat gatcaacaac
    17101 aactgctgga taccctggtt gaacaactca ccgccacgct ggcgctggat cgccatcagg
    17161 aacgtcagca acagttgatc gtgatggaag agcgtgccac cattgcgcgc gaactgcatg
    17221 attctattgc ccaatctctc tcttgcatga agatgcaggt gagttgttta cagatgcagg
    17281 gcgatgcgct gccagaaagc agccgcgaac tgttaagtca gatccgtaac gaactgaatg
    17341 catcctgggc gcagttgcgt gaattgctca ccacattccg cttgcagctc accgagcctg
    17401 gattacgtcc ggcgctggag gcgagttgcg aagagtacag cgccaaattt ggcttcccgg
    17461 tgaagctgga ttatcaattg ccgcctcgcc tggtgccttc gcatcaggca atccacttgt
    17521 tgcaaattgc ccgtgaggca ttaagtaacg ccctcaaaca ttcgcaagcg agtgaagtcg
    17581 tggtgacggt ggcgcaaaac gataatcagg tcaaactgac cgtccaggat aacggctgcg
    17641 gcgtgcctga aaatgccatc cgcagcaatc actacggcat gataataatg cgcgaccgtg
    17701 cgcaaagttt acgaggcgat tgccgcgtcc gccgtcgtga atcaggtggc accgaagtgg
    17761 tggtcacctt tattcccgaa aaaactttca cagacgtcca aggagatacc catgagtaat
    17821 caggaaccgg ctactatcct gctgattgac gatcacccga tgctgcgaac tggcgtaaaa
    17881 cagcttatca gtatggcacc agatatcacc gtggttggcg aagcgagtaa tggcgaacag
    17941 ggtattgaac tggcggaatc tcttgatccc gatctgatcc tgttagatct caatatgccc
    18001 ggcatgaacg gtctggaaac gctggataaa ctgcgcgaaa agtccctctc agggcgcatt
    18061 gtggtattca gcgtctctaa ccatgaagaa gatgtggtca ccgcactgaa acgcggcgcg
    18121 gatggttatc tgttaaaaga tatggaaccg gaagatctgc tgaaagcatt gcatcaggca
    18181 gctgctggcg aaatggtatt aagcgaagca ttaacgcctg ttctggccgc cagcttgcgc
    18241 gctaaccgtg ccactactga gcgcgatgtt aaccagttaa ccccacgcga gcgcgatatt
    18301 ctcaagctga ttgcccaggg tttgccgaac aagatgattg cccgccgcct ggatatcacc
    18361 gaaagcacag taaaagtgca cgtcaagcac atgctgaaga aaatgaagct caagtctcgc
    18421 gtggaagcag cggtatgggt gcatcaggag cgcattttct gattacggtt cccagcgcag
    18481 ttcgtcgttt gacaatgcat cgaacggttc gacaagcgtt agcgtgatct cattggagga
    18541 gacacgctgc ccctggttat cttccaccac caccgacaat cgccagtgat tgctcgcccc
    18601 ttccccgttc tgccagtcag gcatgatcag cgtccagccc tccgcgctgt tggcttgtgc
    18661 gcccggcgtc aaacttaata tctgcgtatc gccctgccaa atcagttgcc gaataccgta
    18721 acgactgcgg atttgtaatt tcagcggcac tgtttcgcca ggttttagat cccacggcgg
    18781 tgtcgccaga aacaccgtta acgtttttcg ctgtcggtac tcaagagtcg gtagattatt
    18841 tcgctgcgga ttatcatagc gactaccacg taacgactga ctttcggcaa cctcgcccgc
    18901 agaaagttgt tttttgagcg gcacaccaaa gcggtaatta agattcagtc cgaggttatt
    18961 ttgattttcg ccactttcac cctgtttatg ctgggccgtc acagtgacta atggcacagg
    19021 ggtgtaattt aatcccagac tcaacgcgac gggattgtga taacccgtac cagagttaaa
    19081 caaatcaaca cgctcaccaa aatactgttc taccctgaca ctggtattga ggtgttgata
    19141 gaacggcatg cgcatccggg ctgtcaggtc atacccgcgc gccatccgtt gttcctgcgt
    19201 ggctgtctgt tcatgccatg cagcaaacgg ctgataaaag tttgccgata gtcgcaaata
    19261 ttcgccccac gcttcggcac caaagcccgc tcgctgaaga ttttcgtcca gcaagttgtc
    19321 ataaaaagtg ttataaccca ccagccagtt gccgcgcgcc cagcgttgcc caacgcccac
    19381 attgctcacc aacccatcat cctgctgagt aagaccaagc tggctccagg tgagataacg
    19441 atcattatct tgtaacggca caaaccagct tccacgactg ccggtgaaat gtccttcgtt
    19501 atccactttg acgtcaacac tggcatttcc ccacggtgat agccaggact ctacgtgctg
    19561 attaacctgt tgactaagcg cgtcgcggac ttttcccaat gcgaaagctt ttgcctgctc
    19621 gccagtatcc agcccgttat cattcatact ggtttcgccg aaatctttca caatttcagc
    19681 aaagtgtttt tccccatcat ggttttcggg agccatgcct aaatccggca gaccatcatt
    19741 gttattatca aagggatttg ccgctttttg ctcgaaggta gattgtgcgt tagctgtacc
    19801 gcctgccgcc agcaagagta aataaaacgg gataatgcga ggaacgaaac ggctcaaaac
    19861 gtcggtaact tccggtaata atgaaaaacc atagccttaa agaataacga ttactggcaa
    19921 cttttgtcgt attttctcgc gctttcagga aaatccttaa aaaggtgacc gattggtcag
    19981 acgctgcaga agcagtaaga tagaagatga gtgtctaatt ccctgaatac aggagtaaat
    20041 aatgcaaaaa atcgtgatcg ttgccaatgg cgcaccttac gggagcgaat ccttgtttaa
    20101 cagcttgcgg ctggccattg cgttacgaga gcaggagagc aatctggatc tgcgtctgtt
    20161 cctgatgtct gatgcggtca cagccgggtt gcgcgggcaa aaaccagggg aaggctacaa
    20221 cattcagcaa atgctggaga tccttaccgc tcagaatgta ccggtgaaat tgtgcaaaac
    20281 ctgtaccgac gggcgcggga ttagcacact tcctcttatt gatggggtgg aaatcggtac
    20341 tcttgtggaa ctggcgcaat ggacgctgtc agccgataaa gtgctcacat tttaatgttt
    20401 gagggggcgt ctgacgcccc actatgaggg aagtttactc aggcgaatcc cggccgtaac
    20461 acaccatccg gataattctc cgccagcagt ttttttaccg ataccagcaa atcattcagc
    20521 ccatcgtcct gcattcccag tttgataagc tcctgttcca gtgaaaacag gtattgtgcg
    20581 ttggttccca gcggaccgct cgccgccgca atcaacggcg cgatgacctg agcgcgagtg
    20641 tcagattcat attctggatg tcgcgggtcc ataataaaca caatggcgtt tactgtgcgt
    20701 ccatcatcaa gatcaagctg acaccaggtt ggcagataac agccggtaat catctcgcgc
    20761 ttccacaaca gggttagttc ctgctccagc gtctcttctg gtagtcgata ggcgacgcct
    20821 gtggtgcgtc cgccctcttt cagtgcaagc atccgtcccg gctggtgcgc agtcccgcgc
    20881 ccggcggtca ggcgcaggca aaacgcgcga tgccatccaa ccagtgtacc ggtgcataat
    20941 tcggtaaact ccagtgccgg attccacatc aacgatccat aaccgaagat ccacaccggt
    21001 ccctcattag gtcgacaagc cagcgtcgcc gccagcgaag ccgcccgttg ttctgctgac
    21061 cataagagtg attcttcaat ggcaccaaat gccgttttac aatcggcatt catcaagaaa
    21121 tcacgcgtta tcaccttaca cctccgccac tgcatacttc cctgcgacaa ctttttggtg
    21181 tcttccggac atttttaccg ctcattttga aaccactaac gggacaatat gcaatccacg
    21241 gaccacttgc aaggcaacac cgcacgaata gctttaacta agctaccggt tttacgattt
    21301 tttatgccat ttatcatcat cccctttggc atattcatgc ttcacagcag cccaggccac
    21361 tttatgcgcg gtttcttcgc gactggcgtc atcacgccga tcttctttat ctttatattg
    21421 atcccatgcg ctgttgaacg cttctttata gatatcctgg gcatgagacg gtagaacgtg
    21481 ctttacgctt tccggcagat cgcttttcgt tttatacggc atcgttgact ccttttacaa
    21541 agttgaacat taagtgtggt caacaatatc tactcttgcc aaaacataag aattttcatg
    21601 tcagactgtt cttaagaaac gctaaaacaa acattgtcta aacgtaatac ttatttttat
    21661 gccccaatga gtaaaaaatc gacagcgggg taaaaattcg taaaatgtta ccgatatcca
    21721 gacactttct gttatttttc ctacctattt tttacatttt cgataaattc atctgcaaag
    21781 ggataaccat aatgtcaaat gctcaagagg cggtaaaaac ccgccacaag gagacttcgc
    21841 ttattttccc ggttctggcg ctggtagtgc tgttcctgtg gggaagcagc cagacactac
    21901 cagtggtcat tgccatcaat cttcttgcgc ttattggtat tttaagtagc gcctttagtg
    21961 ttgtccgtca tgcggacgta ttagcccatc gcctgggaga accttacggt tcgcttattc
    22021 ttagcctttc agtggttatt cttgaagtca gtttgatttc agctttaatg gcaaccggcg
    22081 acgccgcgcc aacgctaatg cgtgatacgc tctattcaat cattatgatt gttaccggtg
    22141 ggctggttgg cttttcatta ttgttgggcg gtcgtaagtt tgccacccaa tatatgaatc
    22201 tgtttggtat caagcagtat ttaattgcgc tgttccccct ggcgataatc gtactggtat
    22261 ttccaatggc tctgcctgcg gcgaattttt caaccggtca ggcgttactg gtagcattaa
    22321 tttctgcggc aatgtatggc gtatttttgc tgatccagac caaaacgcat caaagtttgt
    22381 ttgtctacga gcacgaagat gacagtgatg acgacgaccc gcaccacggt aaaccgtctg
    22441 cccatagcag cgtgtggcat gctatctggt tgattatcca tctgattgcc gttattgcag
    22501 tcaccaaaat gaatgccagc ccgctggaga cattgctcga cagcatgaat gcccctgtcg
    22561 cctttactgg cttcctggtg gctctgttga ttctgtcgcc ggaaggttta ggtgcgttaa
    22621 aagcagtgtt gaacaaccag gtccagcgcg caatgaatct gttctttggt tcagtgttag
    22681 caaccatttc gcttaccgta cctgtcgtca cgctaattgc ctttatgacg ggtaacgaat
    22741 tacagtttgc acttggtgcg ccagaaatgg tggtgatggt ggcctcttta gtgctgtgcc
    22801 atatctcctt ctccaccgga cgtactaacg tgctcaatgg cgcagcgcat ctggcactgt
    22861 ttgccgccta tttgatgacg atatttgcct gacagttata tctcctccga tgacggaaat
    22921 cgggggagat aagataaatt cctgcttatt gtatttcttc atactacctg gacagctcgt
    22981 taatcagaat tgcgagcctg taccacaacc tgcacgaaac cactgcaaca gctgttgaaa
    23041 agccggaatg tactctttat tgcaaacatt tcagggatga agtgaagatg aattgagacg
    23101 tagtatccac accaagccgg aattatccgg tgaggcgcaa tgttgcgggg gctttatccc
    23161 tggtggcatt ggttgctgga aagagaaaac ccccgcacgt tgagaggtaa aacctgacaa
    23221 caaaacgggg gctaatcttg aaaccagaca cttcaagaat agccgccttg ttgtcattac
    23281 aacaaggcgg ctatatgacg ctcgcgcagt ttgccatgac tttctggcac gacctggcag
    23341 caccgatcct ggcggggatt attaccgcag cgattgtc
//
LOCUS       DAJVRF010000057        23237 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJVRF010000057 DAJVRF010000000
VERSION     DAJVRF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.057"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(38..238)
                     /gene="glgS"
                     /locus_tag="OQN13_002033"
     CDS             complement(38..238)
                     /gene="glgS"
                     /locus_tag="OQN13_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCS1615395.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            507..1136
                     /locus_tag="OQN13_002034"
     CDS             507..1136
                     /locus_tag="OQN13_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HCS1615396.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1163..2824
                     /locus_tag="OQN13_002035"
     CDS             1163..2824
                     /locus_tag="OQN13_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCS1615397.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3063..3122
                     /locus_tag="OQN13_002036"
     CDS             3063..3122
                     /locus_tag="OQN13_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1615398.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3439..3498
                     /gene="ibsE"
                     /locus_tag="OQN13_002037"
     CDS             3439..3498
                     /gene="ibsE"
                     /locus_tag="OQN13_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCS1615399.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3618..5051)
                     /gene="hldE"
                     /locus_tag="OQN13_002038"
     CDS             complement(3618..5051)
                     /gene="hldE"
                     /locus_tag="OQN13_002038"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCS1615400.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKQGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5099..7939)
                     /gene="glnE"
                     /locus_tag="OQN13_002039"
     CDS             complement(5099..7939)
                     /gene="glnE"
                     /locus_tag="OQN13_002039"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCS1615401.1"
                     /translation="MKPLSSPLQQYRQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7962..9263)
                     /gene="ygiF"
                     /locus_tag="OQN13_002040"
     CDS             complement(7962..9263)
                     /gene="ygiF"
                     /locus_tag="OQN13_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCS1615402.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9505..10125
                     /locus_tag="OQN13_002041"
     CDS             9505..10125
                     /locus_tag="OQN13_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCS1615403.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10189..11427
                     /gene="cca"
                     /locus_tag="OQN13_002042"
     CDS             10189..11427
                     /gene="cca"
                     /locus_tag="OQN13_002042"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCS1615404.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11590..12411)
                     /gene="bacA"
                     /locus_tag="OQN13_002043"
     CDS             complement(11590..12411)
                     /gene="bacA"
                     /locus_tag="OQN13_002043"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS1615405.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12502..12870)
                     /gene="folB"
                     /locus_tag="OQN13_002044"
     CDS             complement(12502..12870)
                     /gene="folB"
                     /locus_tag="OQN13_002044"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCS1615406.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12975..13592
                     /gene="plsY"
                     /locus_tag="OQN13_002045"
     CDS             12975..13592
                     /gene="plsY"
                     /locus_tag="OQN13_002045"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCS1615407.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13605..14537)
                     /gene="ttdR"
                     /locus_tag="OQN13_002046"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(13605..14537)
                     /gene="ttdR"
                     /locus_tag="OQN13_002046"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            14744..15655
                     /gene="ttdA"
                     /locus_tag="OQN13_002047"
     CDS             14744..15655
                     /gene="ttdA"
                     /locus_tag="OQN13_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCS1615408.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15652..16257
                     /gene="ttdB"
                     /locus_tag="OQN13_002048"
     CDS             15652..16257
                     /gene="ttdB"
                     /locus_tag="OQN13_002048"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCS1615409.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16306..17769
                     /gene="ttdT"
                     /locus_tag="OQN13_002049"
     CDS             16306..17769
                     /gene="ttdT"
                     /locus_tag="OQN13_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCS1615410.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17812..18825)
                     /gene="tsaD"
                     /locus_tag="OQN13_002050"
     CDS             complement(17812..18825)
                     /gene="tsaD"
                     /locus_tag="OQN13_002050"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCS1615411.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19062..19277
                     /gene="rpsU"
                     /locus_tag="OQN13_002051"
     CDS             19062..19277
                     /gene="rpsU"
                     /locus_tag="OQN13_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCS1615412.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19388..21133
                     /gene="dnaG"
                     /locus_tag="OQN13_002052"
     CDS             19388..21133
                     /gene="dnaG"
                     /locus_tag="OQN13_002052"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS1615413.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21328..23169
                     /gene="rpoD"
                     /locus_tag="OQN13_002053"
     CDS             21328..23169
                     /gene="rpoD"
                     /locus_tag="OQN13_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCS1615414.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5652 a         6332 c         5908 g         5345 t
ORIGIN      
        1 aatacaaaca ctaaaacggg ccatcaggcc cgttatatca gtgctctaac tccagctctc
       61 ttgcctgcat catctgttgg caataccagg cataacgtgc gcaaaaccag gttctatgtt
      121 cttcatccat gttaccagta acggcaagaa tatcgaccgg gcgaccttct gcgtgcattc
      181 tggcaaaact acgcgccagg aaatcgaaat tatttaaaga attaagacta tgatccataa
      241 gcacatcctc cggttatcgc atacctttta aggccatatg ctttttctga tttcagtatt
      301 ccccaatcag tgtagtgcct ttaaacataa cgtgcgtaaa tatattgtcc ccgatcacac
      361 tttttagtga aaaataaaaa actatattaa aaaactttct cttccagaac ttctctaaca
      421 tgtgttatta ttaacaaaga taaatgtaag caaggaaact atcccaaagt aaatattgcg
      481 tcactaaatg gacattggag tgacatatga ttttattcgc cgactataat accccttatc
      541 ttttcgctat ttcctttgta ctgttgatcg gccttttaga aattttagcg cttatctgcg
      601 gtcacatgct ctccggtgct cttgatgcac atcttgacca ttataattcc ataaccacag
      661 gtcatattag ccaggcactt cattatctca atatcggaag attgccagct ctcgtcgttc
      721 tctgcttgct ggctggtttt tttggtctta ttggcatcct gttgcagcat gcctgcatca
      781 tggtctggca atcaccactg tcgaacctgt tcgtggttcc tgtcagttta ctgtttacga
      841 ttattgcggt gcattatacc ggtaaaatag ttgcgccctg gattccacgc gaccatagtt
      901 cagctattac agaagaagaa tatattggta gcatggcatt aattaccggt catcaagcca
      961 cttcaggtaa tccctgcgaa ggaaaactca ccgatcaatt tggccaaata cattatttac
     1021 tactggagcc tgaagaagga aaaattttca ctaaaggagt caaagtgtta attatttgcc
     1081 gactttctgc aacgcgatat ttggcggaaa acaatccctg gccacaaata ttgtaattta
     1141 ttaatcaaaa ggaaatttaa taatggatga tattgttaat tctgtgccct cctggatgtt
     1201 taccgcgatt attgccgtat gcattctgtt tattattgga attattttcg ccaggctcta
     1261 tcgtcgcgct tcggcagagc aagcttttgt tcgtactggt ttaggtgggc aaaaagtggt
     1321 gatgagcggt ggcgcaatcg tgatgccgat ctttcatgaa ataatcccca tcaatatgaa
     1381 tactctgaag ctggaagtca gccgctcaac cattgatagc ctgattacga aagatcgtat
     1441 gcgtgtcgat gtagtagtcg ctttctttgt acgggtaaaa ccgtctgtag aaggcatcgc
     1501 cactgcggcg cagacgttgg ggcaacgcac gctatcacca gaagacttac gtatgttggt
     1561 tgaagataaa tttgtcgatg ccctccgtgc aacagctgca caaatgacca tgcatgagtt
     1621 acaggatacc cgcgagaact tcgtgcaggg agtacaaaat actgtcgcag aagacctatc
     1681 gaaaaacggt cttgagctgg aaagcgtttc acttaccaac tttaaccaga cctcgaaaga
     1741 acatttcaat ccgaacaatg cctttgacgc cgaaggttta accaaactga ctcaggagac
     1801 ggagcgccgt cgtcgcgaac gtaacgaagt tgaacaggat gtagaagttg cggtgcgtga
     1861 gaaaaaccgt gatgcacttt cgcgcaagtt ggagattgaa caacaagaag cgtttatgac
     1921 gcttgagcag gagcagcagg ttaaaacccg tactgccgaa cagaatgcac gtattgcggc
     1981 ttttgaagct gaacgtcgtc gtgaagcaga gcagacgcga attctggctg aacgacaaat
     2041 tcaggaaacg gagatcgagc gcgagcaggc cgtccgctca agaaaggttg aggctgaacg
     2101 tgaagttcgt attaaagaga tcgaacagca gcaggtcacc gaaatcgcca accagacgaa
     2161 atcgatcgct attgccgcca aatcggaaca acagtcccag gcagaagcgc gtgctaacct
     2221 tgcactcgca gaagcagtaa gtgcgcaaca aaacgtagaa accactcgcc agaccgcaga
     2281 agccgatcgt gctaaacaag ttgccctaat cgctgccgcg caggatgcag aaaccaaagc
     2341 ggttgaactg accgtgcggg cgaaagcaga aaaagaagcc gcagagatgc aggcggcggc
     2401 tatcgttgag ttagccgaag ctacacgtaa aaagggtctg gcggaagcag aagcacaacg
     2461 tgcgctgaac gatgctatca acgtactttc tgatgaacaa accagcctta aattcaaact
     2521 ggccttgttg caggcgctac ctgcggtaat agagaaatcc gttgagccga tgaagtccat
     2581 tgacggtatc aagattattc aggtcgatgg tctgaatcgt ggcagcgctg cgggtgatgc
     2641 aaacacgggt aatgtggggg gcggaaacct agcggaacaa gcattatcag ccgctctctc
     2701 ttaccgcaca caggcaccgc tgattgactc cttgctcaat gaaattggcg tttcaggcgg
     2761 ctcactggcg gcattgactt cacccttaac ctcaacaact cccgtcgccg aaaacgtaga
     2821 ataaattttc agctatgaac gtccctggca caaaaagccg gggatttttt atatctgcgt
     2881 tccgctaaaa actgcaaatg atcaggccgt tgcaacgttt tgcgtgaccg ctcggggaag
     2941 gcaaaactgc ctctgggaaa gcgttgcgcg aggtccggcg ctcatcaaca atcggggagt
     3001 cagcaaaggg ctgaaacggg aaagcccctc ccgaggaagg ggccttacag aaggaaaggg
     3061 ttatgatgaa gctcgtcatc atactgattg tgttgttact cgtaagtttc gcagcttatt
     3121 aacagccaat cagagggggg agaaatcctc cctcaccctt gttcctttac tttaggttga
     3181 aaaaaaatca gcgtcaagaa ggtttcatta acacatatcc ctggcccaaa aagccgggga
     3241 ttttttatat ctgcgttccg ctaaaaggtg caaatgctca ggacgttgca gcgttttgcg
     3301 tgatcgctcg gggaaggcaa aactgcttca gggaaagcgt tgcgcggggt ccggcgctca
     3361 tcaacaatcg gggagtcagc aaggggctga aacgggaaag cccctcccga ggaaggggcc
     3421 ttatagaagg aaagggttat gatgaagttc gtcatcatac tggttgtgtt gttactgtta
     3481 agtttcccga cttactaaca actgatcaga gggggaagaa atcctccctt acccttgttc
     3541 ctttacttta ggttgaaaaa acaacagcgt caataggcct gcctgctaca aagcgagatc
     3601 tgtgaaccgc tttccggtta gcctttttta tcctgttgga tcttcttgat gatgttagtg
     3661 gtcgagcaac cgtcttcgaa gttgagcacc agcacttcgc cgccgttggc ccagacttct
     3721 ttactcccgg cgatctcttc tggtttatag tcgccgccct tcaccagtaa atctggcagg
     3781 atcccggcga tcaagcgctg cggcgtgtct tcttcaaacg acaccaccca gtcgaccgct
     3841 tccagtgcgc ccagcacaat catacgctgt tcgagcgggt taacggggcg ggaatcccct
     3901 ttcagccgtt tggtggaggc atcgctgttc acagcgacaa tcagacggtc accctgtttg
     3961 cgagcatttg ccagatagga gacgtgcccg gcgtgcagga tgtcaaagac accgttggtc
     4021 attaccacct tttcgccgcg tttacgcgct gccgctacgg ccagcttcag ttcctcttcg
     4081 gtcatcacgc caaagccggt atctgcgcgc ccacgcactg cattttccag ctcgatcggc
     4141 gaaaccgtgg aggttcccag tttgccgacc accacgccag ccgccgcatt ggcaaagaag
     4201 caggcttctt ccagcgaatt acccgctgcc agcgttgccg ccaggacgcc aatcaccgtg
     4261 tcgcccgcac cggtaacgtc atacacttcc tgcgcctggg ttggcatatg cagcggcgct
     4321 ttacccggtt gcagcagcga cataccctgt tcggaacggg tcactaacag agccgagagt
     4381 tcgtaatcgg caatcagttt catgccgcgc tcaacaatct cttcttcggt cttacattta
     4441 ccgacaacag cttcaaattc cgagagattc ggcgttaaca gcgtagcgcc gcggtagcgc
     4501 tcaaaatcgg taccttttgg atcaatcagc accggaacgc ccgctttacg cgccagttgg
     4561 atcatctgct gtacgcttgc cagcgcacct ttggcgtagt cagaaagcac cagcgcgcca
     4621 atcgaactca gcgcctgatt aatccgctcg tgcagcggct gcggatcaac gccttcgaaa
     4681 ccttcttcga aatccagacg gatcagctgt tggttgcggg aaagtacccg taatttggtg
     4741 atggtcggat gcgtcggtac agaaacgaag tcgcatttga cgttgacgtc ggccagagat
     4801 ttactcagcg cgcgcgctgc atcgtcaatg cccgtcaacc cgaccaggcg tgcattagca
     4861 ccgagagaag cgatattcat cgccacgtta gccgcgccgc ccggacgttc ttcgatggta
     4921 ttcactttaa ccacgggcac cggcgcttcc ggcgagatac ggctggaggg accgtaccag
     4981 taacgatcca gcattacatc accaaccacc atcactcctg cacgttcaaa ctctggcagc
     5041 gttactttca ttcctgtctc ctgagagatt caaaatttgc gcgcgataat accatacttc
     5101 attcttccac cagccacttc tgccagcttg cccgcaccag atcacgctct gcggtgaagc
     5161 aatcccccga cacatggccc ggcaattcct gtaatgccag atgatgaagt tcatcgcgca
     5221 gcgtcgtgta agcacgggtc agcgccatcg cttcctgctc ttccataatg tcgttttgcg
     5281 ccagcagctc cagaatacgc acgttgtctg accagcgcgt taacttcggt ttttcatgag
     5341 cgtagcgcaa caccagatat tgggtaataa attcgatatc ggtaattccg ccttcatcag
     5401 ctttgatatc aaagcgatcg cgatgtttat tgccgagatg agcgcgcatt ttctcgcgca
     5461 tttcccgcac ttccgtttgc agagttctac cttcacgcgg cagcgtcata atctcgcgac
     5521 gcactgcgtc aaagtgcgcg gtgagctgcg gatcgccgta cactacccgc gcacgcacca
     5581 gcgcctgatg ttcccacgtc caggcctcgt ttttctgata atcggcaaat gcttctgtgg
     5641 aggtaaccag cattcccgcc gccccggacg ggcgcagccg cgcatccact tcgtacaaaa
     5701 tgccggaaga ggttcgcgtg ctgaacaggt gcataatgcg ttgcgccaga cgaagataaa
     5761 actgtcgtcc gtcgatttcc cgctcgccgt cagtcatcgc atccattggg caatcatgga
     5821 ggaagatcag gtcaagatcg gaactgtagc ctaactccca gccgcccagc ttgccgtagc
     5881 cgaccactgc aaaaccacgc ccttcgcgat cgttgaggtg attcggctta ccgtagcggg
     5941 caaccatttg aacccacgcc tgctgaacga cggcatctat catggcttcc gccagccagg
     6001 ttaagtgatc gctcactttc atcaccggta gcgtaccagc gatatccgct gcggcgatgc
     6061 gtaacagctg cgcctgtttg aactgacgca gcgcctcaag ctgttgctct tcgtcatctt
     6121 ccggcacgcg cagcaaatac tggcgcaact catcgcggta ggcatcggtc gccgtcggct
     6181 ggtaaagagt gtttggatcg agcaattcat ccagcaataa tggataacgc gccagctggc
     6241 tggcaatcat cggcgatgcg gcacacagag aaatcaaatg gttaagcgcc gcagggaatt
     6301 cactgagcaa ttctaaatag gtggtgcggg taacaatccc caccagcaag gcggtaatgc
     6361 gcgacagcgt gacagcagcg tcttcacgcg cacagacatc acttagcaga tgcggcatca
     6421 gatggtcgag cacctgacgt cctcgcggcc cgatggtgcg cttatccagc tctttgcgga
     6481 aatcggaaat cagcgttagc acctgtttgc gatcatcctc gctaagatgc gccagcactg
     6541 gcgtagtgtc atcttcctgc aacgcatcct gccacagctc acgccactgt tccgacagcg
     6601 actcttcctg agtttcactt tcgtcgtcgc caatcaattc attaaacacc cggcgcacat
     6661 tgttcatatg tgcggtcagc gccccggtca gttgcggcca gtcagcaaag tccatcgccc
     6721 acgccagccg cgcacggtta agctcatcag aaggaagcgt ctgggtttgt tcgtcgttaa
     6781 tgctttgcag caggttttcc agacgccgca ggaacagata cgccactcgc aattgttcag
     6841 catcgttttc agaaagcaga tgcagcgcag caatggcgct gagcgttggc agtaaagagc
     6901 acgattgcag cgacggttcg cgcccgccgc gaatgagctg gaatacctgg acgataaatt
     6961 caatttcgcg aataccaccc gcgccaagtt tgatgttgtc ggtcaaaccg cgccgacgca
     7021 cttcacgggc aatcattcct ttcatgttgc gcagcgactg aatcacgctg aaatcgatgt
     7081 aacgacggaa aacaaacggg cgtagcatcg cacgcaactc gttagcatag acgccttcgc
     7141 tatcgcccat aatccgcgct ttgaccatcg cgtaacgctc ccagtcgcgc ccctgctcct
     7201 ggtaataatc ctccaacgcg gcaaagctca gcaccagcgg gccactttcg ccaaacggac
     7261 gcagtcgcat atccacgcga tagacgaagc catcctgcgt tggttgatcc agcactttaa
     7321 tcagccgctg ccccatgcgg gtaaaaaact gcgcgttatc cagttcccgg cgtccgccct
     7381 gcgtacaacc gtgttccggc caggcaaaaa tcagatcgat atcagaggag aaattcagtt
     7441 ccccgccgcc cagcttaccc atgcctaaga tcagcagcgg ttgcgcttcg ccctgcgcat
     7501 tgcacggcgt tccccactcg cggcagcagg cgtcatacag ccagtcacgc gccgcaacaa
     7561 tcagcgtctc cgccagataa ctgagctgct gcaatatgct ctcttcagta accagtgcca
     7621 gcgtttgcgc ccatgcgatg cgcaccataa tgcgccgccg gaatagccgc agctcgcgca
     7681 ttaacccggc ttcgtcactc acattactga gcgcctcctg caaccatgca gcgtaatgct
     7741 gccattcatc ggcctgcggc ggttggcttt ccagttccgt caaccactct cgatgcgcaa
     7801 ttatgctgtc ctgcacaaaa tcactgaaag taagtactga cttcgcctgt gcacttagtg
     7861 gttcctcggc taaaggctct ggcagccgct caacaacggt ctgcctgtac tgctgtaacg
     7921 gtgaagagag cggcttcata agcgatttta tccttgcctg attaacgttt tccgctgtgc
     7981 aaccagaacg gttcctgatt gtttgcctca ttacggaaat gctcaatttc aatgcgttgc
     8041 ccggtcgcaa tagcgtgacg cagcccctgc cagttctcca gccaggcatg cgcgacgaca
     8101 ggatcatagt aacccgccag caacagtatt gagtcaatgt cacgcgtcag gcgtggcaac
     8161 tggtcacggt agagatcgcc taacggctgg cagaaaacgc ttttcagttc agcggcatgg
     8221 tgggaaagat ggatatcggc aaagcgtttg aaggagtcgc tgattttgcc ctgcgctttg
     8281 gcatctaaaa atggctgcca tgctttgctt accaaccatt cggtcaacgc cagcttcgcc
     8341 attgccgttt cggtagagta gaccgccgtc acggcagaaa ccgcagaagc aatggtcgct
     8401 tcgcattggg tcagcaaatc acgtaagtga gtgctcgctt tacgcggcac aataccaccg
     8461 aacagcatca gcgtatgacg gaccaggcta atggctgcca gcacctgttc tttcgccgca
     8521 tcgttaccgc gtacccacag ttcttcatga tactgccatt gcgctaacgc cagctcgagc
     8581 gccgcttcca gcccctgttc tacatcggct tttgccgcaa catgcaaaat ggtggtcggt
     8641 ttgatttcac gcgccggatt gccctgcgcc agatgatagc cacgcgccgc tttgctcagg
     8701 ctgccctggc gtaatccggt ttgcgatacc agttggttcg ccagtttcag caccgcgcgc
     8761 gtgtcgccgc taagcagttc cagttccagc tcacagatag gttcagcaaa ttcccccgct
     8821 ttcacctccc cctgatcgag agcgatttca attcggctgc catcgaccgc caccagccat
     8881 ttttcgcgat aaaaatcggt gctgaacagc ggttgtacgc gggaagcgag atcggcaggc
     8941 aattcaccgt tcggccagac ttccgaggga aactgcgcca ggtcgagcgt cggttcgctc
     9001 aacgccacgt tatattccgg gcgctggtgt aagccgcctg tcactcttcc tgcaactttc
     9061 atggtcatct catagcgacc gttttcgcca cgaatacgta agcccatatc gtgcctacgc
     9121 agccagttat ccggcgtttc gtagtaaata ttcagcaact gcacggggtc atggtgctcg
     9181 ccgcccagcg tattgagatg gtcacgcaac gcctcaacgg cactgtgatt aacaataaac
     9241 tttaattcga tttcctgagc catggccttt acttatcggt tatgtcacat aagtgacgat
     9301 gaacggcgaa cttaatgcga tcttttttgt cagtagatag tattttgcgc caaattgcca
     9361 tgcaacgggc aatttgacgg gcgtaaaagt ttgaagcagt ggcaagacct gacacagagg
     9421 attccgattg atgtcgaatg ctttgcgtag cgacactgat gccactacta tcgttccact
     9481 ttccatgaca ataacgacag cctgatgcca aaattacgcc tgatcggatt aactttactc
     9541 gcacttagcg cgactgccgt ctcacacgct gaagaaacgc gctatgtttc tgacgaactg
     9601 aatacctggg tccgtagcgg tccgggagat cattatcgcc tcgtgggcac ggttaacgcc
     9661 ggcgaggaag tgaccttatt acaaactgac gccaacacca attatgccca ggtgaaagac
     9721 agctctggcc gtaccgcctg gatcccgttg aaacaactta gcactgagcc aagcctgcgc
     9781 tcccgtgtgc cagatctgga aaatcaggtc aaaaccctga ccgataaact caccaatatc
     9841 gataacacct ggaatcagcg caccgcagaa atgcagcaaa aagtggcgca gagcgacagc
     9901 gtgatcaacg ggttaaaaga agaaaatcag aaactgaaaa acgagctgat tgtcgcgcag
     9961 aaaaaggtcg atgccgccag cgtacagctg gatgacaaac agcgcaccat catcatgcaa
    10021 tggtttatgt atggcggtgg cgtgctgggg cttggcttgc tgctcggtct ggtactgccg
    10081 cacctgatcc caagccgcaa acgcaaagat cgctggatga actaaatcgc cttcttcgtt
    10141 gcactgtctt attattagga attgtgtatt tgacgaaggg atgatggcgt gaagatttat
    10201 ctggtcggtg gtgctattcg ggatgcattg ttagggctac cggtcaaaga cagagattgg
    10261 gtggtagtcg gcagtacgcc acaggagatg ctcgacgcgg gctaccagca ggtaggccgc
    10321 gattttcctg tgtttctaca tccgcaaacg catgaagagt atgcgctggc acgtaccgaa
    10381 cggaaatccg gttccggtta caccgatttt acttgctatg ccgcaccgga tgtcacgctg
    10441 gaagatgatc ttaagcgtcg cgatctgacc attaatgcgc tggcccagga cgataacggc
    10501 gagattatcg acccgtacaa cggtctgggc gatctgcaaa atcgtctgtt gcgccatgtt
    10561 tcccccgctt ttggcgaaga tccgttacgc gtactgcgcg tggcgcgttt tgctgcgcgt
    10621 tatgcccacc tcggttttcg tattgccgat gaaactctga cgctgatgcg cgagatgacc
    10681 catgcgggtg aactggaaca cctgacgcct gaacgggtat ggaaagagac ggaaagcgcc
    10741 ctcaccaccc gcaatccaca ggtgttcttc caggtactgc gcgattgcgg cgcactgcgc
    10801 gttttattcc cggaaattga cgcactgttt ggcgttccgg cccctgccag gtggcatccg
    10861 gaaatcgaca cgggtattca taccttaatg acgctctcaa tggcggcgat gctgagtccg
    10921 caggtcgatg tccgtttcgc aactttatgc cacgatctcg gtaaaggact gacgccgcca
    10981 gaactttggc cgcgtcatca tggtcacggt ccggcgggtg tgaaattagt ggaacaactc
    11041 tgccagcgtc tgcgcgtgcc aaatgaaatt cgcgatttag ccagactggt ggctgagttt
    11101 cacgatctca tccacacctt cccaatgctg aacccaaaaa ctatcgtcaa attgtttgat
    11161 tccatcgacg cctggcgtaa accgcagcgt gtcgagcaac tggcgctgac cagcgaggct
    11221 gacgtacgcg gcagaaccgg ttttgaatca gcggactacc cgcaaggccg ttggttgcgc
    11281 gaagcctggg aagtggcgca gtcagtgccg acaaaagcgg tcgttgaagc gggatttaaa
    11341 ggtgtggaga ttcgcgagga gctgacccga cggcggattg cggcggtagc cagttggaag
    11401 gaacaacgtt gcccaaagcc tgactgagta taaaactaaa cgttaacacg aaccatcccc
    11461 tcgccccttc ggggagaggg ttagggtgag gggaacagac cagcaccggt gcgaacaatc
    11521 acccccaccc cggccctctc cctacagagc gagggagaaa accaggcctg ctcccgaacc
    11581 ttttctcaat caaaagaaca cgacgtacac cgcagccgcc acaataaagc gataaatggc
    11641 gaacgggata aacgaaatgc gcttaatcaa ttgcaggaag gttttaatcg ctatcagcgc
    11701 caccacaaaa gcggtgataa acccaacggc aaacatcgag atatcgccgg ttgtcaggaa
    11761 gccccagctt ttgtagagat cgagcgccgt tgcgcccatc atcatcggta ccgccagcag
    11821 gaacgagaac tcggaagcag cgtaacggct cacgcccatc agcatcccac ctgaaatggt
    11881 ggccccggaa cgggagaaac ccggccacag cgccagacac tggaaacagc caatcataaa
    11941 cgcctgacga taggtcatat catcaagacc cggcgcacgc ggctctttcg gcttcaggca
    12001 ttcggcggca atcagcaaca aaccgccaac gaccagtgca tacatcacat ttatcgggtt
    12061 aaacaatgac ttaatcgtgt cgtggaacaa cagccccagt accaccgccg gaatcatccc
    12121 cagcaaaatg tggatcagcg ttaaacgacc tttgctttct ccttcgtgct gcaacgggcg
    12181 gccaaagtgg atgccaatca ggccaaacag acgccgccag aacatcacta ctaccgccag
    12241 aattgatcct aactggatca caacttcaaa ggttttcgcc gtgtcgccct cgaaccccaa
    12301 caagtgaccg acaataatca tatggcccgt gctggatacc ggcagaaatt ctgtcaatcc
    12361 ttcgaccaca cccaatattg ccgctatcag cagcgagtgc atatcgctca tcaataaacc
    12421 cctaaattat taaaatgtac cgcttgtccg gactactgca gtaagaccag gttataaccg
    12481 tttggtttaa cagctgtaaa attaattatt ttctttcaga ttattgccac gctcaatgat
    12541 tacaccaaca ttcgccgccc gcgccattgc gcctggcttg ctgagtttga tgcgcaccca
    12601 cggcgagttg aagcgtgcta acagcagctc cgccacctct tcagccacgc gttccaccag
    12661 cgcaaaacgc gccccctcga cgtggctgac caccgtttct gcaatgtcag cgtaactgag
    12721 gcaatccgcc acatcatcac ttttcgccgc tttacggtta tcccatgcca tttcgatatc
    12781 gaacactaac ttctgttcga tggtctgttc ccagtcgtaa acaccaatag tggtgattac
    12841 cgaaagttgc tctataaata caatatccat cacgtcctgc ctgctttttg gctaaccgga
    12901 taccacttcc ggcgaaatgt gcgtattatc cacagattca tcgttgaaca cgaattttca
    12961 aaacggaaca gcttatgagt gcaatcgcgc ctggaatgat cctcatcgcg tacctctgcg
    13021 gctccatttc cagtgccatt ctggtttgcc gcttgtgtgg gctgcccgat ccgcgaacca
    13081 gcggctccgg caatccaggc gcaaccaatg tgttacgtat cggtggcaag ggagcagccg
    13141 tagcagtact gattttcgac gttctgaaag gaatgttgcc cgtctggggc gcgtatgaat
    13201 taggtgtcag ccccttctgg ttaggcttaa ttgccatcgc cgcctgtctt ggacacatct
    13261 ggcccgtttt cttcggattt aaaggaggaa aaggcgttgc taccgctttt ggtgccatcg
    13321 cacccattgg ctgggatctc accggagtaa tggcgggaac ctggttactg accgtgctat
    13381 tgagcggata ctcgtcgctg ggagcgattg tcagtgcact gattgctccg ttttatgtct
    13441 ggtggtttaa gccacaattc accttcccgg tttcgatgct ctcttgcctg atcctgctgc
    13501 gtcatcatga caacatccag cgtctgtggc gtcgtcagga gacaaaaatc tggacgaaat
    13561 tcaaaagaaa gcgcgaaaag gatcccgagt gatttctggt ggatctacat gacctgatag
    13621 ccttcatcgg gcttgcccag ccgttgctgg caccatgccg ccagaaattc cacgcagaca
    13681 cgtaatttca tgctgcgata gagcggctcc cggtaaacag cccagatatt ggcgctttgt
    13741 gcatactccg gcaatacttg caccagtttg ccactctcca gaaacggcaa cacatcccac
    13801 tcggaacgca gcataatccc tttgccctcc agcgcccatt gcaacactat ctcgccgcta
    13861 ttggaggaaa gatgcccgct aaccttcacc gattttttct cctgaccgtt ccccaactcc
    13921 catattccgt gggtcatatc gcgctctttg gtcaccagac agtcatgacg acttaattcc
    13981 tgtaatgatt gtggctgcgg atatttttgc agatactcag gtgctgcgca taatattctt
    14041 ttatttttcg ttaacagatg cgcaatataa taatcaggaa tttcgtcatt aatacgaata
    14101 tccagatcaa tattatcctg cactaaatca atttgccgat cgaacaattc aaaatgcacc
    14161 tgtaactcag gataattgcg cataagttcg gtaatggctg gcgcaatatg gctgcgccca
    14221 aaaccaaaac tgcaaccaat acgaatcatc ccctccgggc gcgttttgat ttacgtgacg
    14281 tcatcgacca gtcgctgata ttgggtgagg atttccagcg catgttcgta acaacgttgg
    14341 ccactttcag tcagcgccac gccgcgagcg gagcggttca gcagcgttgt tgccagcgta
    14401 ttttcaagga tttggatccg cttggtgaca aatgcaggcg tctgcccaag cgtcgccgct
    14461 gcggcgctaa aactcccgct gtggacgatt tctaccagta cctgcaagtc tttggctaaa
    14521 ggccagctat tcagcatcgt taattatccg cagttgtgat aagcgcagtg tatttcgcaa
    14581 aacattgcca ttccctgctc tggcgcaata ttcattctta ttttcgcgtt gattcacgaa
    14641 ttgttaatta catattcacg gtggcaaaaa atataaaacc acatttttag tggtagtttg
    14701 tggcggtgaa tttttccaga caaatacaaa aactggagtt gccatgatga gcgaaagtaa
    14761 taagcaacag gcagtgaata agttgacgga gattgtcgct aactttaccg ccatgatttc
    14821 tacccgaatg cctgatgacg tggtggataa actaaaacag ctaaaggatg ccgaaacgtc
    14881 gtcgatgggg aaaattatct accatacgat gttcgacaac atgcaaaaag cgattgacct
    14941 gaatcgtcct gcctgtcagg acaccgggga gattatgttc ttcgttaaag tcggttcccg
    15001 cttcccactg cttggcgagc tgcaaagcat actcaaacaa gccgtggaag aggcaaccgt
    15061 caaagcgcca ctacgtcaca atgcggtaga aatttttgac gaagtaaaca ccggcaaaaa
    15121 taccggtagc ggcgtaccgt gggtcacctg ggacatcatc cccgacaatg acgatgcgga
    15181 aatcgaagtt tacatggcag gcggcggctg cacgctacct ggccgctcga aagtgttaat
    15241 gccgtcagaa ggctacgaag gcgtggtgaa attcgtcttc gaaaatatct ccaccctcgc
    15301 cgtaaacgcc tgtccaccgg tactggtggg cgtgggcatc gccacctcgg tggaaaccgc
    15361 cgccgtactc tcgcgtaaag ccattttgcg cccgattggc agccgccatc ccaatccaaa
    15421 agcggcagaa ctggagctac gcctggaaga aggactcaac cgtctgggga ttggtccaca
    15481 agggctgact ggcaacagtt cagtgatggg cgtacatatc gaatctgccg cccgccatcc
    15541 gtcaaccatc ggcgttgctg tctctaccgg ctgctgggcg catcgtcgcg gcacactgct
    15601 ggttcatgcc gatctcacct ttgaaaatct gtctcacacc cggagcgcgt tatgaaaaag
    15661 atcctgacaa ccccgatcaa agctgaagat ctgcaagata ttcgcgtcga cgatgtgatc
    15721 tacctgaccg gtacgctggt gacctgccgt gacgtttgtc accgccgctt gatcgaactg
    15781 aaacgtccga tcccttacga tctcaacggc aaagcgattt tccacgctgg ccccatcgtg
    15841 cgcaaaaacg gcgataaatg ggagatggtt tccgttggcc cgacaaccag tatgcgtatg
    15901 gaaagttttg aacgtgaatt tatcgagcag accggcgtga aactggtggt tggcaaaggc
    15961 ggaatggggc cgctgaccga agaaggctgc cagaaattca aggcgctaca tgtgattttc
    16021 ccggcaggct gcgcggtgct ggcggcaacc caggtggaag agattgaaga agtgcactgg
    16081 acagagctcg gaatgccgga gtcgctgtgg gtctgtcggg tcaaagagtt cggcccgctg
    16141 attgtctcta ttgataccca cggcaacaac ctgatagccg aaaacaaaaa gctgttcgcc
    16201 gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg tccattacat caaataaccc
    16261 tcccggagag gctcgccccc tctccttttt cgcaggcata acacgatgaa accttccact
    16321 gaatggtggc gataccttgc gccgctggcg gtcatcgcca ttattgctct aattccggtt
    16381 cccgcagggc tggagagtca tacctggctc tactttgccg tttttactgg cgtgatcgtt
    16441 ggactgatcc tcgaacccgt gccgggtgcc gtggtggcga tggtgggtat atccatcatc
    16501 gccatactct ctccctggct gctgttcagc ccggagcagc tcgctcagcc aggctttaaa
    16561 ttcactgcaa aatccctctc gtgggccgtt tccggttttt ctaattcggt tatctggctg
    16621 attttcgccg cctttatgtt tggcacaggc tatgaaaaaa ccgggcttgg acgccgcatt
    16681 gcgctgattc tggtgaaaaa gatgggacat cgcacgctgt ttctcggcta tgcggtgatg
    16741 ttctccgagc tgatcctcgc acctgtaaca ccgtccaact cggcgcgtgg tgcggggatt
    16801 atctatccca tcatccgtaa cctgccaccg ctctatcaat cacaaccaaa cgacagcagt
    16861 tcgcgcagca ttggctcgta catcatgtgg atggggattg ttgccgactg cgtgaccagc
    16921 gccattttct tgacggcgat ggcaccaaac ttgctgttaa ttggactgat gaaaagcgca
    16981 tctcacgcca cgctgagttg gggcgactgg ttcctcggga tgttgccgct cagtattttg
    17041 ctggtgctgc tggttccctg gctggcttat gtgctgtacc cgccggtact gaagtctggc
    17101 gatcaggtgc cgcgctgggc agagacggaa ctgcaggcaa tgggcccgct ctgttcgcgt
    17161 gaaaaacgga tgctggggct gatagtaggc gcgctggtgc tgtggatttt cggcggtgat
    17221 tatatcgatg ccgcgatggt cggttacagc gtggtggcac tgatgctgct tctgcgcatt
    17281 atcagttggg acgacattgt cagtaataaa gcggcgtgga acgttttctt ctggctggcc
    17341 tcgcttatca ccctcgctac cggactcaac aacaccggtt ttattagctg gtttggcaaa
    17401 ctgttagcag gcagcttaag cggttattcg ccaacgatgg tgatggtggc gttgattgtg
    17461 gtgttttatc tgctgcgcta ctttttcgcc agcgccacgg cgtatacctc cgccctcgca
    17521 ccgatgatga ttgccgctgc gctggcgatg ccggaaatcc cgctgccggt gttctgcctg
    17581 atggttggcg cggcaattgg tctggggagc attcttacac catacgccac cggacccagt
    17641 ccgatttact acggtagtgg ttatctgcca acggcggatt actggcgact gggggcgatt
    17701 tttgggctga tattcctcgt gttgctggtg attaccggct tactgtggat gcccgtggtg
    17761 ttgctttaag tgaggacggt gccggaaatt gctctccggc acccaatagt tttacgcagc
    17821 cggtaactcc gccagcggcc agcgcggacg cacgctaacg ccgagatccg ccgtcgcgcc
    17881 tgctttaaac cgcaccattc cggcataggc gatcatcgcg ccgttatcag tacaaaactc
    17941 cggacgcgcg tagaacactt cgccgcggcg ttttttcatc atttcagcca gcttcgcccg
    18001 taacgtgcgg ttagcactca cgccgcccgc catgaccagt cgcttaaagc ccgtctgatc
    18061 caacgctcgc ttacacttaa tcatcaacgt atcgaccacc gcatcttcaa aggcgcgggc
    18121 gatatcagca cgcgtctggt cgtcggtgcc gttgtcacga atcgtgttcg ccgcaaaggt
    18181 tttcagacca gaaaagctga aatccagccc cggacggtcg gtcatcggac gcgggaaaac
    18241 aaagcgcccg gcagtaccct gagccgccat tttcgacagt aacggtccgc caggataatc
    18301 cagccccagc agcttcgcgg ttttatcaaa cgcttcaccg gcggcatcat cgatagactc
    18361 gccgagcagc tcgtactgac caatgccagt cacgctgatt aactgcgtat gaccgccgga
    18421 caccagcagc gcgacaaacg gaaattccgg cgggttatct tccagcatcg gcgctaacag
    18481 atgcccttcc atatggtgta ccgggattgc cggaacgttc caggcaaacg ccagagaacg
    18541 ccccacggtt gcgccaacca gcagtgcgcc gactaatcca gggcctgcgg tataggccac
    18601 agcatcaatg tcttttgccg ttaagccaga ctcctttagc gccgcctgga tcaacggtac
    18661 ggttttgcgc acgtgatcgc gggaggccag ttcaggcacg acgccgccat agtcagcgtg
    18721 caatttcacc tgactataca attggttggc taacaaacct ttttcatcgt cgtaaatggc
    18781 gatgccggtt tcatcgcagg aagtttcaat acccagtaca cgcatgactt gttttacctc
    18841 gctttattac cgcgcagtgt aggaccaatg cgggttgatg taaaactttg ttcgcccctg
    18901 gagaaagcct cgtgtatact cctcaccctt ataaaagtcc ctttcaaaaa ggccgcggtg
    18961 ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca ttttgaaata agctggcgtt
    19021 gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca catgccggta attaaagtac
    19081 gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa gcgttcctgc gaaaaagcag
    19141 gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa accgactacc gaacgtaagc
    19201 gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact ggctcgcgaa aacgcacgcc
    19261 gcactcgtct gtactaattc cccgagagcg ttgctctccg atcagaccga gttgtagttg
    19321 taaggccgtg cttccgaaag gaatgcgcgg cttattttcg tttatgaatt gctaaaaatc
    19381 ggggcctatg gctggacgaa tcccacgcgt attcattaat gatctgctgg cacgcactga
    19441 catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag cagggcaaga atttccacgc
    19501 gtgttgtcca ttccacaacg agaaaacccc gtcattcacc gttaacggtg agaaacagtt
    19561 ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc gacttcctga tgaactacga
    19621 caagctcgag ttcgttgaaa cggtcgaaga gctggcagca atgcacaatc ttgaagtgcc
    19681 atttgaagca ggcagcggcc ccagccagat cgagcgccat cagaggcaaa cgctttatca
    19741 gttgatggac ggtctgaata cgttttacca acaatcttta caacaacctg ttgccacgtc
    19801 tgcgcgccag tatctggaaa aacgcggatt aagccacgag gttatcgctc gctttgcgat
    19861 tggttttgcg ccccccggct gggacaacgt cctgaagcgg tttggcggca atccagaaaa
    19921 tcgccagtca ttgattgatg cggggatgtt ggtcactaac gatcagggac gcagttacga
    19981 tcgtttccgc gagcgggtga tgttccccat tcgcgataaa cgcggtcggg tgattggttt
    20041 tggcgggcgc gtgctgggca acgatacccc caaatacctg aactcgccgg aaacagacat
    20101 tttccataaa ggccgccagc tttacggtct ttatgaagcg cagcaggata acgctgaacc
    20161 caatcgtctg cttgtggtcg aaggctatat ggacgtggtg gcgctggcgc aatacggcat
    20221 taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc gatcacatac aactgttgtt
    20281 ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac cgtgcaggcc gcgatgccgc
    20341 atggcgagcg ctggaaacgg cgctgcctta catgacagac ggccgtcagc tacgctttat
    20401 gtttttgccg gatggcgaag accctgacac gctggtacga aaagaaggta aagaagcgtt
    20461 tgaagcgcgg atggagcagg cgatgccgct ttccgcattt ctgtttaaca gcctgatgcc
    20521 gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg agtacgctgg cactgccatt
    20581 gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt cgtcaggaat taggcaacaa
    20641 attaggcata cttgatgaca gccagcttga acgattaatg ccaaaagcgg cagagagcgg
    20701 cgtttctcgc cctgttccgc agctaaaacg cacgaccatg cgtatactta tagggttgct
    20761 ggtgcaaaat ccagaattag cgacgttggt cccgccgctt gagaatctgg atgaaaataa
    20821 gctccctgga cttggcttat tcagagaact ggtcaacact tgtctctccc agccaggtct
    20881 gaccaccggg caacttttag agcactatcg tggtacaaat aatgctgcca cccttgaaaa
    20941 actgtcgatg tgggacgata tagcagataa gaatattgct gagcaaacct tcaccgactc
    21001 actcaaccat atgtttgatt cgctgcttga actgcgccag gaagagttaa tcgctcgtga
    21061 gcgcacgcat ggtttaagca acgaagaacg cctggagctc tggacattaa accaggagct
    21121 ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga tcgggaagcc cccgacagcc
    21181 gcactgagag gcagcggcaa atatataagt acgccctcgt aattatcgtt ggcggtaaac
    21241 aaccgttgga tttcagcgtt aacggctgaa ggacatcggg tcaatcgccc aacaccaacc
    21301 tcatgaaata agtgtggata ccgtcttatg gagcaaaacc cgcagtcaca gctgaaactt
    21361 cttgtcaccc gtggtaagga gcaaggctat ctgacctatg ccgaggtcaa tgaccatctg
    21421 ccggaagata tcgtcgattc agatcagatc gaagacatca tccaaatgat caacgacatg
    21481 ggcattcagg tgatggaaga agcaccggat gccgatgatc tgatgctggc tgaaaacacc
    21541 gcggacgaag atgctgccga agccgccgcg caggtgcttt ccagcgtgga atctgaaatc
    21601 gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa tgggcaccgt tgaactgttg
    21661 acccgcgaag gcgaaattga catcgctaag cgtattgaag acgggatcaa ccaggttcaa
    21721 tgctccgttg ctgaatatcc ggaagcgatc acctatctgc tggaacagta cgatcgtgtt
    21781 gaagcagaag aagcgcgtct gtccgatctg atcaccggct ttgttgaccc gaacgcagaa
    21841 gaagatctgg cacctaccgc cactcacgtc ggttctgagc tttcccagga agatctggac
    21901 gatgacgaag atgaagacga agaagatggc gatgacgaca gcgccgatga tgacaacagc
    21961 atcgacccgg aactggctcg cgaaaaattt gcggaactac gcgctcagta cgttgtaacg
    22021 cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg ctcaggaaga gatcctgaaa
    22081 ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc agtttgacta cctggtcaac
    22141 agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac gtctgatcat gaagctctgc
    22201 gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc tgtttaccgg caacgaaacc
    22261 agcgatacct ggttcaacgc ggcaattgcg atgaacaagc cgtggtcgga aaaactgcac
    22321 gatgtctctg aagaagtgca tcgcgccctg cagaaactgc agcagattga agaagaaacc
    22381 ggcctgacca tcgagcaggt taaagatatc aaccgtcgta tgtccatcgg tgaagcgaaa
    22441 gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac gtctggttat ttctatcgcc
    22501 aagaaataca ccaaccgtgg cttgcagttc cttgacctga ttcaggaagg caacatcggt
    22561 ctgatgaaag cggttgataa attcgaatac cgccgtggtt acaagttctc cacctacgca
    22621 acctggtgga tccgtcaggc gatcacccgt tctatcgcgg atcaggcgcg caccatccgt
    22681 attccggtgc atatgattga gactatcaac aaactcaacc gtatttctcg ccagatgctg
    22741 caagagatgg gccgtgaacc gacgccggaa gaactggctg aacgtatgct gatgccggaa
    22801 gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa tctccatgga aacgccgatc
    22861 ggtgatgatg aagattcgca tctgggggat ttcatcgagg ataccaccct cgagctgccg
    22921 ctggattctg cgaccaccga aagcctgcgt gcggcaacgc acgacgtgct ggctggcctg
    22981 accgcgcgtg aagcgaaagt tctgcgtatg cgtttcggta tcgatatgaa caccgaccac
    23041 acgctggaag aagtgggtaa acagttcgac gttacccgcg aacgtatccg tcagatcgaa
    23101 gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg aagtgttgcg tagcttcctg
    23161 gacgattaat cggtaggccg gatcaggcgt tacgccgcac ccggcactag tccctctgca
    23221 caaacgccac cttttcg
//
LOCUS       DAJVRF010000058        23092 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJVRF010000058 DAJVRF010000000
VERSION     DAJVRF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23092
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.058"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..156)
                     /gene="cas6e"
                     /locus_tag="OQN13_002054"
     CDS             complement(<1..156)
                     /gene="cas6e"
                     /locus_tag="OQN13_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCS1615415.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREEL"
     gene            complement(138..884)
                     /gene="cas5e"
                     /locus_tag="OQN13_002055"
     CDS             complement(138..884)
                     /gene="cas5e"
                     /locus_tag="OQN13_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HCS1615416.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(895..1950)
                     /gene="cas7e"
                     /locus_tag="OQN13_002056"
     CDS             complement(895..1950)
                     /gene="cas7e"
                     /locus_tag="OQN13_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HCS1615417.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(1962..2498)
                     /gene="casB"
                     /locus_tag="OQN13_002057"
     CDS             complement(1962..2498)
                     /gene="casB"
                     /locus_tag="OQN13_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HCS1615418.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(2495..4056)
                     /gene="casA"
                     /locus_tag="OQN13_002058"
                     /pseudo
     CDS             complement(2495..4056)
                     /gene="casA"
                     /locus_tag="OQN13_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
     gene            complement(4154..6853)
                     /locus_tag="OQN13_002059"
     CDS             complement(4154..6853)
                     /locus_tag="OQN13_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HCS1615419.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGWPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGL
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKE
                     DSSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
     gene            complement(7048..7200)
                     /locus_tag="OQN13_002060"
     CDS             complement(7048..7200)
                     /locus_tag="OQN13_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCS1615420.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(7465..8199)
                     /gene="cysH"
                     /locus_tag="OQN13_002061"
     CDS             complement(7465..8199)
                     /gene="cysH"
                     /locus_tag="OQN13_002061"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCS1615421.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(8274..9986)
                     /gene="cysI"
                     /locus_tag="OQN13_002062"
     CDS             complement(8274..9986)
                     /gene="cysI"
                     /locus_tag="OQN13_002062"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCS1615422.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(9986..11785)
                     /gene="cysJ"
                     /locus_tag="OQN13_002063"
     CDS             complement(9986..11785)
                     /gene="cysJ"
                     /locus_tag="OQN13_002063"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCS1615423.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            12104..12466
                     /gene="queD"
                     /locus_tag="OQN13_002064"
     CDS             12104..12466
                     /gene="queD"
                     /locus_tag="OQN13_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCS1615424.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            12544..13815
                     /locus_tag="OQN13_002065"
     CDS             12544..13815
                     /locus_tag="OQN13_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1615425.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            13806..14066
                     /locus_tag="OQN13_002066"
     CDS             13806..14066
                     /locus_tag="OQN13_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCS1615426.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            14083..14658
                     /locus_tag="OQN13_002067"
     CDS             14083..14658
                     /locus_tag="OQN13_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCS1615427.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGGMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(14806..15666)
                     /locus_tag="OQN13_002068"
     CDS             complement(14806..15666)
                     /locus_tag="OQN13_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCS1615428.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(15663..16442)
                     /locus_tag="OQN13_002069"
     CDS             complement(15663..16442)
                     /locus_tag="OQN13_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCS1615429.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(16420..17829)
                     /locus_tag="OQN13_002070"
     CDS             complement(16420..17829)
                     /locus_tag="OQN13_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1615430.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVDFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(17851..19305)
                     /locus_tag="OQN13_002071"
     CDS             complement(17851..19305)
                     /locus_tag="OQN13_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCS1615431.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(19375..20191)
                     /locus_tag="OQN13_002072"
                     /pseudo
     CDS             complement(19375..20191)
                     /locus_tag="OQN13_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            20509..21786
                     /locus_tag="OQN13_002073"
     CDS             20509..21786
                     /locus_tag="OQN13_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1615432.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIFQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAIASSK"
     gene            21813..23092
                     /locus_tag="OQN13_002074"
                     /pseudo
     CDS             21813..23092
                     /locus_tag="OQN13_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         5509 a         6152 c         5692 g         5739 t
ORIGIN      
        1 gagttcttcc cgacgataaa gaaattgcct ttctttgccg ccagggaaaa gatcccacag
       61 ccactgatgc atcacatatt caccgcgatc caccagatgc agcaactgcg caggcgaaag
      121 ctggccggta tgtaaggtta ttcttgagag gtacatggtt cctccttgct gagccacggc
      181 ccctgattga cggtgcgttc cccaaacagc cactgctgac gatttaaagg aacatcccgg
      241 cggcgtaata ttttgctcgc gaccaggccg tcgtgtttcc cttcccacca gcattcatcc
      301 tgaagtttcg ggagtgagac tttcagttta tggaaatgat cctgatactg ttggtatgca
      361 ttacataagg catcacacgc gttgccttcg agcagtaacg gcgcaagagg taatgccagc
      421 ggatgacttt ttcgccccag ataaagcgga aaaactggat ggcgtaaacc gtcctgcaac
      481 tgttcgaggc tgtaaggcgc atcggctgtt gttgccaccg ccaccatcca ccaggcatcg
      541 gtgtagtagt cgcgccggga gataatcgcg ctcaaaagct cagggtcgct caactcttca
      601 cgacggctga aataacgtgc tttacgcacc tcttttggca tctggaccgt gtgataatcc
      661 cgtgcccagc gcgggttacg gctggcgcaa accaccagtg aatagtggcg attaaacgcg
      721 tttaagcgtt cggtatcatc acgccgaatc cctaccccgg cagccagtag ccccagcaat
      781 gctgagcgcg aaggaagttc atgggtatga cgaacttcac ctggagcatc gacaccccag
      841 gacgccatcg gcccatgaag ctgaaaaatc agatattggc tcataagccg ccccttacgc
      901 gcagataaag tccagcacgt ccttcatgct tccctgtttg ttcatcacgt caaaacttgc
      961 gcattcggtc ttctgttcgt agaccgtatt catgttttcg cgaagcgttg taatacgctg
     1021 caccgccaca tctaactgac gtgtgccatt aatgggttca tagaaagccg ccgccagaga
     1081 gcgtggttga tcggtgcctt tttctgccag cgcccaggaa gcataggcac ggctggcaaa
     1141 gctattttgt ttaccggttg gggaaacttt aagcgcggct tccgtaaaag cgcgtatggt
     1201 ctgattagct aacgcttcgt caccgccgag gttttctacc agcagatctt tatcaatgca
     1261 gatataggtg tagaaaagtg ctgaaccgaa tccggtttct ccgagatgcc cggcaccggc
     1321 atcttcagag gcctggcgca aatcatcaac ggcggtgaaa aaatcatctt cgacaatcgt
     1381 ttcactgaca ccaaacgcat gcgcgacctg gcaggcggct tcaacattaa actcaggttt
     1441 attcgccagc atacgaccaa acatagcgat atctaccgct atacggtctt tacgtaacaa
     1501 cgcgagatct tcctcttttg gcgcgcgctt ttcttctgcc agttgatgag caagcgcttt
     1561 tacggcgtca aattctgccg ggctgatatg aactaattgt tcagtttcgg cgttagtgag
     1621 cggatctttt ggttttttgt catttttagc tttcccaaga taatcagcaa tttttgccgc
     1681 ccattcgatg gcttttttat cttcaatccc tttctcaatc aggatagttg ccgcctcacg
     1741 cgcaatacgc ccactgcgaa taccaatatg gcccgccagt gcctgttcaa aaagtgcaga
     1801 agtgcgccac gcacgtttca gactttgcga ggaaacgcgc agacgcgttg ctccacccag
     1861 caccacagtt tttggcgcac cggtgtcatc acggttaagg ttggcagcag ggtaagcggt
     1921 taacacatga agctgaataa atgttgtcat gagatagtcc tttattgatt ttgttcgtta
     1981 tcggcgtcgc cagcctggta atattccagc gcccagcgga tacggataaa ttcggttggc
     2041 cgctgctggc ggcggtgatg attgagcagg tcgtcgctct cctggcacca gcggaaaatg
     2101 ccatctgcca gggagtcaag attgacggaa ccgtttaata atctgacggc gcggcgtaac
     2161 tggcgcagta attcatccgg tgtttttact gctgacaggc gagtaaaacg cccctttgac
     2221 attaccgccg ccagttgcgc ggcaaaaggc tgccgttcat caatggcttt aacattggcg
     2281 ctaactgcgg ctatcagcgc cagtgcggtg atacgccact caggttcatc ctgccacttt
     2341 atttgtctgt tcttcagaaa caggcgaaat ccatccgtca gacaaacatc attaaccgtc
     2401 gtactacgcc gcagactggc acgttcgccg cgtttctcct gcaattcctc gtgccatttg
     2461 cgcagcgtgg ctttgtgctc ctcttttaca atactcattc agcagcctcc tgctttttct
     2521 ccctggcggc tttagcactt tgcttctccg ccgatgttgt aaaatatttc ttgcgcgcgg
     2581 tcatgatgcg ttccaaatca acgggctcat aaggattggt gaatacacgc tcgtcaaaat
     2641 cctgacgcgc gaataaccaa atttccttat tccatttgct gagtaattca tccgcatcct
     2701 gaccttcttc aatttggcgc actaacctca ggaagcgatg ctgagttttg ttccagaagt
     2761 cgatatccac aaaactaaaa tcaccccttg caccttttgg atcggagaac catgcttctt
     2821 tcaatgcact ccgtaacaga cttagaatcc gtgaagccgt ttgcgcagcc agccgcagtt
     2881 tcggtatctg gccttctttt tattgagcag cagcgggaaa tgatgttcgt accaacagcg
     2941 cgctttcatg ttgtcgaaat catagccaaa tccccacagg cccacttttg cctgcttgag
     3001 actgctggca ttaaagagtt tcaccaccag cgcgggaagt tccgtattgt tttctgactt
     3061 acccgtttcg ataaggccta accagtcgcg ccagattaaa ccgcccggtt gtggtttgac
     3121 cgagtaaaac tcgccgccct ctttaagtag tacacggtaa ggcgttaagg gatgctgcca
     3181 catggcatag ttagcgccgt aatttttggt cgtcatcagg ctcagaagcg cgtcgctctg
     3241 ctcaccgcaa atatcgcagt tgccgactgt ggtggtatta aaatcaatac gaatacggcg
     3301 aggcattccc cagtacgcct ggagtttatt gacctgctca tcggttacca ccgcaccggc
     3361 cagttcgctg atgcgcgtcg ggccaagcca ggggaaaacc agatcgtcaa attttttggg
     3421 tagcggtaaa tcggcttcat cctgcggcat gacattgatc cacagtttgc gccacaaggg
     3481 agtttgttga ttaccctgat attcctgtaa ttcaatcaga gtggtcatcg gcccaccgcc
     3541 gcgtaaaccg gtgcggtagc ctttgccacc tgacggcgca ttcaactgta gggagaacag
     3601 agctaacgca gagcaatgtg ggcacaggta ttccgttaca ccacgcttga taaagtggtc
     3661 tttattaaac ttcgttgttt gagcgccggg aatctcaggc agtagcgaag cgaccggaac
     3721 tttatctccg gtgagcgcct cgaaatcctg tataaatgaa ggggaatctg gcccaaactg
     3781 gaaagcatgt tctaatgaca gcaatgcttc ccgtagcttt tcagcttcca gtccgtcttc
     3841 ccagatatca tcccaacgac gatgatcttt tggcgcgaag ctggtttgta gtagccccag
     3901 caaaaactgc cataccgcac cctggagatc tgcccgcggc gcagaaatat cgacaacatt
     3961 ttcatccgcc agatcgactg gcgccagttt gcctgtcgtt ccgtctttaa aacgaacggg
     4021 caaccacggg gttgtcagaa gtgaaaacga gttcatacgc atcctctcgt aaaaaagacc
     4081 atcccaggtc ggttgtccat ctttccatcc ttttcggtgt atcaactatt cagagagtcc
     4141 tgtcgcagac atctcaacca accttgccaa tcaatccttc cgtagccgaa tacccacaac
     4201 tttcgtcatc gttgactaaa ataacgtttg ccatctccgg atcttgtcgc tgttcaatgc
     4261 accacagcct gaacgcctct ccctccagta aagaaaactc atcccgatgt tttttccacc
     4321 agcttcggcg cactctgaca acgctcattt cccatgcgtg agcaccggtg gcataaggct
     4381 tcaccacacc gtcaatacag gtagccagcc acagggaaac agactcctca gccagacgtg
     4441 tcgacagttt ttctgggagg taatcgttga tattggcggc atagccgggc ctgaagttca
     4501 gcacaaactt tttagccatt gcgcgatcac agtaatattt gcccacttgc tcctgctcgc
     4561 tgcgggcaaa tccttccggc attaccacgt cctcaccgta gacggattca ataagaagcc
     4621 gggctgcgtg tggcatctga atagcacctt gctcacgcag cacacgctgc gtcagccaga
     4681 ttcgcccatg atcgggatag acaaatgcac tgttacgcat ggcactgcca aaccattcgt
     4741 cgccaggaga gtcgtcccag acgggagcca gaatcagcaa ttcagggggg gaacgctcgt
     4801 cctttccgtc acgctttaac tgaccattaa tatcgcggat atgccgctgt aatcgtcctg
     4861 ctcgctgaat cagcaaatca acaggggcca ggtcggagat catttcgtcc aggtcacaat
     4921 cgacactctg ctctaatacc tgagtacaaa taaggacttt tccggcacgc tgtgagctat
     4981 cttctttacc aaagcgtgcc agtgtctccg tttcaattcg ctggcgatcg ctaaaagcaa
     5041 agcggctatg aaaaagtgaa aggctggaag caggaatgac gccgcgggca agcagctgac
     5101 gatgaacctt aatagcgtca tcgacagaat tccggatcca ggcgatgcat ttcccctgac
     5161 ttaccgccga ttcgatacgc gcaatacact cttgttcact atgaagccaa cccacgctga
     5221 cgctacgctc aacgtctttg cgcgtcgcta cccggtgtga gttcacatcg gatttcgtga
     5281 catgcgtcag ccaggggtaa tcatcctttt ccaggaacgg agcttcttgc aggccctctg
     5341 taccccgcgc aaaggcagcg acgagtttgt cgcgctgctg ttgagataac gtggcagaaa
     5401 gcaaaataac gctgtttccg ccacgcgcct gccgctcgat caacccttca agaatgcacg
     5461 acatgtaagc gtcacaggcg tggatctcat cagccagcag gattttgtta ctcagcccca
     5521 gaagccgcag attattatgt ttgaacggca tcaccgccat catcgcctga tccagcgtgc
     5581 cgacgccaat ttcagccagc aacgccttct tgttactgtc ggcaaaccag gccgcacatc
     5641 cctgactgaa cgtttgttca tccggttctt ctgacccgac taaatcacca gaccagagtg
     5701 attcattaaa gcggtccatt aatgtgcggg cactatgtgc cagcaccagg ctggggcggg
     5761 attctggcga atagaaagca agccaggttt tgaccagccg atcgtacatg gcattggccg
     5821 ttgccattgt tggcagacca aaaaacagcc cctgagcctt ccccgcagct atcaacctgt
     5881 gcgccaggat aagcgccgct tctgttttac ctgcgccagt cacatcttcc agaataaata
     5941 actgcggtcc ttgctggctg atatccagat ccagcacctt ttgctgtaac ggtgtcggat
     6001 tctcaataaa aggaaacagc gtattaattc cgttgaaagg cgcgactttt gcttttagag
     6061 gaaagacggt taaggcgttt tgagcctgaa ctaaagtttt ctgccagtaa cctttaatat
     6121 ccattgggtg tgcgacgcgt ggaaaaaatc gcgttgacga acccgtccag tctgcgagca
     6181 cgacggttgc agagatatac caggaaaggt gttttataag ttcaatgccc tcatcatcat
     6241 cccagaatgc ggggatctct atgagcggaa acagtggttt gatttcaagg agaaaatctc
     6301 gtgctgcagc tttgtcttca ggcagaaaat tatccaattc atccatacga tcagggggcc
     6361 aaccatgatg ccctgtcgtc acgggcatcc acatttctat cactcgccta agtttgcgcg
     6421 gagagagcga agatgaagga agtaactcct gacattcact taaataatga ttccacaacc
     6481 agtagcccag cgttgaatga gagatttttt cgtaattttt tctggcacct tcctgaatct
     6541 tgagttcagg gggcaggtaa agttgttgaa aagagcgggc aaattttcca atatcgtgcc
     6601 agcacagcaa ccaggcgaaa aattgagccg cctgttcctt gtcagaaatc cctaattgac
     6661 gaaaataatc agccagcccg aagcaatttc ttttaaccat taaatagccc atcgcggcta
     6721 catccagcga atgccagcaa agaaggtgat agtcgtcgcc cccgtctttc tcgccgcgtc
     6781 gggtttttcc ccagaaatca aaaaaagtca caatattttt atccttcagt aaacttaaag
     6841 gatatttacg catatattaa aaattatctg tgatatatat cattcaataa aatatttaca
     6901 ttatgaataa tcaaataatt ttcatccgta atatatatga ataataaaca taggcataat
     6961 attcacataa ataatataaa ttaaaaaagg gtgcaggaga ccaaaccatc acaattcagt
     7021 acgcggggtt ttacccccgc gtggtggcta cttcttcgat tcgtaagcga gaactgcctt
     7081 aaactccata ccacgttctc tgatactcaa ctcacacaac gagtcgccta ccatcagcgt
     7141 gaatcccagt actgtaaaac acagcactat gattgccaca agggcatatt ttgtcagcat
     7201 gtcttgcctc cctgcaaaga agagactaac attcgaattg tttgggttcg aacgctggcc
     7261 tcaggttgat agaaatatcg cctggggctt ttgtccatct ggaacctcgc gaatgcttaa
     7321 cgccagacag cctcaagcac ccgacgccat tctatacctg ataattcttc cagcggcgtt
     7381 cacgtttaaa atcaggaata ctgctcgcaa aataaaaacg cccccatcat ttctgacaga
     7441 ggcgtttaat ttatccggca atatttaccc ttcgtgcaat ccacattccc tttttaagcc
     7501 aaagaaacgt gtttcttctt ccgacatgcc aggttcccat ttacgggttg tatgggtatc
     7561 accgaccgat aaatatcctt catcccataa tgggtgatat ttcaggccat gtttttgcag
     7621 gtactgataa atagttcggt tatcccagtc gataatcggc agcactttaa atacgccacg
     7681 ctgaattgcc agcaccggta aattggcgcg actgccggat tgttcacggc gcaggccagc
     7741 aaaccaggtt tgcgcgttaa gttctttcag agcccggttc atcggttcga ctttgttgat
     7801 gtcattgtac ttttcaatgc cttcaacgcc ctgctcccac agttttccgt agcgtgcttc
     7861 ctgccaggct gcgctttcgg tagcacggta cactttcagg ttgagcttga gtttgtccgt
     7921 taactcgtca ataaagcggt aggtttccgg gaacaagtaa cccgtatcgg tgaggatcac
     7981 cggaatatcc gggcgaattt gattcaccag atgcaggctc accgccgcct gaatgccaaa
     8041 gctggaagaa agtacatatt caccgggcag attatccagc gcccaggcta cgcggccttc
     8101 agcgtccagt ttttccagtt cggcgttagt ttccgccagc gccagaatac gatctacctt
     8161 cggcagttcg ttcagggcgt ttagatcgag tttggacata gattcctcac tgtttgcctt
     8221 gcctgatgcg acgctcacgc gttcttatca ggcctacaag accgggctga tggttaatcc
     8281 cacaaatcgc gcgccggatc gagcaccggg cgaatgatgc ccgcacgcac cgtaaagtcg
     8341 ccgaagcctt cacccgcttc gcgctctttc gcccagcgcc ctatcagttc atcaagcgac
     8401 gccaggattt ccggctcggt gatgttttct ttatacatcc gtgggatacg tgtcccaatg
     8461 cggttgccgc caagatgcag gttgtagcga cccggcgctt tacccaccag gcccacttcc
     8521 gccagcatcg cgcgaccaca accgttcggg cagcctgtta cacgcatcac gatatgctca
     8581 tcgctgacac catgtttcgc cattaaatta tcgatgttgt cgataaaaga cggcaggaaa
     8641 cgctctgctt ccgccatcgc cagcgggcaa gtcgggaatg acacgcaggc catcgagttt
     8701 tcacgctgcg gcgtgacggc attcattaac ccgctctctt tggcgatctt ctcgatcttc
     8761 gctttctcgc tttccggtac accggtgatg atcagattct ggttcgccgt aatgcggaaa
     8821 tcgcctttgt ggatcttcgc gatctccagc aggccggttt tcagcggacg ccccggataa
     8881 tcaaggatgc gaccattttc gataaacagc gtcaggtgcc agttatcatc aatgccctta
     8941 acccagccaa tacgatcgcc tcgtccggtg aactcatatg gacggatcgg ttcaaatttg
     9001 atccccgcgc gacgctccac ttccgcttta aacgtctcaa cccccacgcg ctccagcgtg
     9061 tatttggttt tggcattttt acgatcggtt cggttacccc agtcacgctg agttgtcacg
     9121 acggcttcgg ccaccgccag cgtatgctcc agcggcagat agccaaactc gctcgccgtg
     9181 cgggcgtagg ttttcttgtt gccgtgttcg atagaaagcc caccgcccac cagcaggttg
     9241 aagcctacca gcttgccgtt ttcggcgatt gccacgaagt tcatgtcgtt ggcatgcaga
     9301 tcgatatcgt tctgcggcgg gatcactacc gtggttttga acttacgcgg caggtaggtc
     9361 tgaccaagga tcggttcttc atcagtagtg gcgacttttt cctggtcgag ccagatctcc
     9421 gcatacgcgc gggtacgagg caacagatgc tcagaaatct tcttcgccca ctcgtacgct
     9481 tccgcgtgca gctgcgactc gtaagggttc gaggtgcaga gtacgttacg gttcatgtcg
     9541 ttagctgtcg ccagcgcatc aagaccgacc gagtgcagca tctggtgcac cggtttgacg
     9601 ttctttttca gaatgccgtg gaactgaaac gtctggcggt tggtcaggcg aatgctgcca
     9661 tagatggtgt tttcaccggc aaatttgtcg atcgcctgcc actgtttagt ggtaatcacc
     9721 ccacccggca gacgacagcg aagcagcatc gcgtggcgcg gctccagctt ctgttcagca
     9781 cgttcggcac ggatgtcgcg gtcatcctgc tgatacatgc cgtggaagcg gatcagcagg
     9841 aagttgtcgc ccttaaagcc gccggtcaga ccgtcgttta aatcttccgc aatggtgccg
     9901 cgcaggtagt tgctttcaag cttcatgcgc tcggcgtctg tcagttttcc ttcgaccact
     9961 aaaggccctg gatgtttttc gctcattagt agacatctcg ctgataacgg cgctctacgc
    10021 gcagctcact taaaaattca tccgccgctt cggtgtccat gccaccaaat tcggcaatca
    10081 cttccagaag tgcctgctca acgtctttcg ccatgcgatt agcgtcgccg cagacataaa
    10141 tgtgggcacc gtcattgatc cagcgccaca gctccgcgcc ctgttcgcgc agtttgtctt
    10201 gtacataaac tttttctttt tggtcgcgcg accaggcaag atcgatacgc gtcagcacgc
    10261 cctctttgac gtagcgctgc cactccacct ggtagaggaa atcttccgta aagtgcgggt
    10321 tgccaaagaa cagccagttt ttacccggcg cttcgtcggc ggcgcgttgc tgcataaagg
    10381 cacggaacgg cgcgatgccg gtaccggggc caatcataat cactggcgtt tgcgggttag
    10441 cgggcaggcg gaaattgtcg ttatgttcga taaatacgcg gacttcgccc tcttcttcca
    10501 cgcgatccgc gaggaagctg gaggcaccac cggcacgggc gcggccttcc acgtcgtaac
    10561 gcaccacacc aacggtgacg tgtacttcgt tctcgacttc cgcctgcgag gaggcgatgg
    10621 aatacaggcg cggggtcagc gggcgcagca ggttaatcag cgcttcggca tccagttgcg
    10681 ccggagagaa acgcaccatg tcgacaatcg gcgtggtcgc ggcgtaatgc tgtaacttcg
    10741 ctttatcgcc caccagcggc agcagcgttt cgctgcgggt aagcgtggca tagttctcaa
    10801 caatgttggc ggtgttgacg gtcagttcga agtgccactg tagcgcttcg ttcagaggca
    10861 acgttttgcc ctcgacggtg acaggttcat cgcctttcag ccacagcagt tcgacaagtt
    10921 ctttcaccag tgccgggtcg ttctgatacc agacacccag cgcgtcaccc ggctggtaac
    10981 gcaggcctga atcgcctaag tcaatttcga tatgacgaac gtctttttca gagttacgcc
    11041 cggtaatttt ctggttaacc gaaaggctcg ccaccagcgg cgcgtctttg ctgtacgggc
    11101 tggtgtggat ttcatttacc gcgccagtag cgacggattg cgaaggtgcc gcgacaggcg
    11161 cacgcgattt aagcgcatca accacgcggg cgcgccactc gctggcagca gcctggtatt
    11221 caacatcggc atcgacacgg tcgagcaggc gttcaccacc cagttccgcc agcttgctgt
    11281 cgaaatcttt cccggactgg cagaaaaatt cataagagct atcgccgagg ctaaacacgg
    11341 caaacgcggt gttttccagc tttggcgctt ttttggagaa caggaactta tgcagcgcga
    11401 cggcttcttc cggcggttcc ccttcccctt gcgttgacgt cactacgatg agcagttttt
    11461 cgctggcgat ttgtttgaat ttatagtcgc ccgcgttcac cagcttaacg ttcagttttg
    11521 ctgctaataa atcatcgcgt aatgcttcag caacccggcg cgcattgccg gtttgcgagg
    11581 cggagataat agttataccc ggcatttctg cggctggcgc tggcgtcgct gcaagcgcag
    11641 caggctgctg attgagtacg ccccagaaat agccagaaac ccaggcaagc tgggtaggcg
    11701 ttaaatcggt cgtggccgcc tgaaggcgtg ccagttgctc cgggttcaac ggaagcaacg
    11761 cggaaggtgg gacctgtgtc gtcatgcgtc gttatgttcc agtaagcaaa gctgtttctg
    11821 cgccctgtca gcgcccataa aacagaagag aagataaggt taacggggtg aacggtgtgg
    11881 attaaagacg ggatagcgat aactaataac caaatcgact aacctgtttt agcaatagtc
    11941 tttaacaaca aaatagatta accaacccaa tgaaaaacaa atgaatttag ccaatcatta
    12001 agataaatca gcgattttgc gcaacaaagt cgttttagat aatgcgaaaa aacagccttt
    12061 ccggtactct acggcggttt tatcgtcctg tagagaaatt atgatgtcca ccacgttatt
    12121 taaagatttc accttcgaag ccgctcaccg cttaccacac gtcccggaag ggcataaatg
    12181 tggtcgcctg cacgggcatt cctttatggt gcgactggaa attaccgggg aagtcgatcc
    12241 gcatacgggc tggattatcg atttcgctga actgaaagcg gcgtttaagc caacttatga
    12301 gcgcctcgat caccattatc tcaatgatat tccaggtctg gaaaacccaa ccagcgaagt
    12361 tttagcaaaa tggatttggg atcaggttaa acccgttgtg ccgctgttaa gtgcggtgat
    12421 ggtaaaagaa acctgcaccg cagggtgtat ctatcgcggc gaatgataag agtgtgtcgg
    12481 cggtcaattt cccttaagta acgctatgtt agggtgttgt gttctggata tctggggcat
    12541 gacatggaag acgactgcga cattattatt attggtgccg gtattgcagg caccgcttgc
    12601 gcgttacgct gcgcgcgagc gggtttatcc gttttgttac tggaacgcgc tgaaatcccc
    12661 ggcagcaaaa atctttccgg cgggcggtta tatacccatg cactcgcgga actcctcccg
    12721 caatttcatc tgaccgcgcc tcttgaacga cgcatcactc acgaaagcct ttccctgtta
    12781 acgcccgatg gcgtaacgac gttttccagc ttacagcccg gcggtgaatc ctggagtgta
    12841 ttacgtgcac ggttcgatcc gtggctggtt gccgaagccg aaaaagaagg tgtcgaatgc
    12901 atccccggag cgacggtgga tgcactgtat gaagaaaacg gcagagtctg tggcgttatt
    12961 tgtggtgacg atattctccg cgcccgttat gtggtgctgg cagaaggtgc caacagcgtc
    13021 ctggctgaac gtcacgggtt agtgactcgt cctgctggcg aagcgatggc gttggggatc
    13081 aaagaagtgc tgtcgctgga aacatccgct attgaagaac gttttcatct ggagaataac
    13141 gaaggcgcag cgttgctgtt cagcggcgga atctgtgatg acttacccgg cggcgcattt
    13201 ctttatacta atcaacaaac gctctcgtta gggattgttt gcccgctctc ttcccttacg
    13261 caaagtcgtg ttccggcaag cgagctgctg actcgcttta aagcgcatcc ggcagtgcgc
    13321 ccgcttatca aaaacacgga atcactggag tatggtgcgc atctggagcc agaaggtggc
    13381 ttgcacagta tgccggtgca atacgccggt aacggctggc tgctggtggg cgatgcgttg
    13441 cgcagttgcg tcaataccgg aatttccgtg cgcggcatgg atatggcgct gactggcgca
    13501 caggcggcgg cacaaacgct aataagcgcc tgccagcacc gcgagccgca aaatctgttt
    13561 gcgctttatc atcacaacgt agagcgcagc ctgctatggg atgttctaca acgttatcag
    13621 catgttccgg cgcttttgca acgccctggc tggtatcggg cgtggcctgc gttaatgcag
    13681 gatatttccc gcgatttatg ggatcagggt gataaacctg ttccaccgct gcgccagtta
    13741 ttctggcgtc atttacgtcg tcatggcctg tggcatctgg cgggcgatgt tatcaggagt
    13801 ctgcgatgtc tgtagcccat aatctctggc gcgttgctga tgcgccgcac attgttccgg
    13861 ctgactccgt tgagcgtcag atggtacaac ggttgattaa cgcctgtccg gcaggtcttt
    13921 tttcgctcac accggaaggt gacttacgcg ttgattatca cggctgcctg gagtgtggca
    13981 cctgccgttt gctgtgcgac gaatccacac tacaacagtg gcgctatccg ccttccggat
    14041 tcggcatcac ctaccgcttt ggataaataa taaggataat ttatgcccct cttacaccta
    14101 ctccgccaga atccggtgat tgctgccgtt aaagataatg ccagcctgca actggctatc
    14161 gattctgaat gccagtttat ttccgtgttg tacggcaata tctgcaccat cagtaatatc
    14221 gttaaaaaga ttaagaacgc cgggaaatat gcttttattc atgttgattt gctggaaggc
    14281 gcgtcaaata aagaagtcgt tattcagttt ttgaaactgg tgaccgaggc ggacggcatt
    14341 atcagtacca aagcctcaat gttgaaagcg gcaagagcgg aaggtttttt ctgtattcat
    14401 cgcctgttta ttgttgattc aatttcgttt cacaacattg ataagcaagt tgcgcaatcg
    14461 aatccggatt gtattgagat cctgccaggc ggtatgccca aagtgctggg ctgggtgaca
    14521 gagaaaatcc gccaaccgct gattgccggt gggctggtgt gcgatgaaga agatgcgcgt
    14581 aatgcgatta acgcgggtgt cgtggcgctt tccaccacga ataccggggt ctggacgtta
    14641 gcgaaaaaat tactttgacg ggataatcgt aaccaattga atttggtttg atttgtaggc
    14701 cgcacgccac atccgacatt cagcgcctga tgcgacgctt gacgcgtctt atcaggccta
    14761 caagtcccca ggccagaacc gtaggcagga tcatccggca acacgttatt ggcaatctgc
    14821 gtgaatgttt gtcaccagtg cctccagcac cactttccag tcatcaacca cgccaacatc
    14881 tgcctgtgaa aataccgcag cgctggcgtc atgattaatc gccaccacaa acttgctatt
    14941 gcgtacgccc gccattaacg ctgctgcgcc agaagcgcca acaacaatac acacctcagg
    15001 tgccagcaga tgtccggaaa taccaatcac tttttcagca tcaacgccac cgttcatcac
    15061 tcgcgcccga ctgtagccca cttcggctcc cagcttttct gccagcatag cgatttcctg
    15121 gttatctgcc tctccgccct gccccaccac cagcacccgt ctggcctcag ctaatgggtc
    15181 gcgagtaaca tttttcagat cctctgtgct aaccagccag tccggaagcg cacccgggac
    15241 aatgttcagt tgttgcattc cagagggcaa cgtggcgttt ttcgctgctc ccgcctgacg
    15301 cgccagggaa aggcatagcg ggcgtttttc tgtttgcagc gtcgccgtta gcgcatttcc
    15361 ccagtgggat ttgcgcacgc ttacggttgg aatatctaac gaagtcacct ggcagatgct
    15421 tgcaccatgt aaacgccagg ccagccgggt cgacagttca tcgccaaacg ttcccggcgg
    15481 aaagagaaca acgtccgcag gcgttcgctg ccactgttcc accaacgcat ccagcacctg
    15541 ttccgccact acaggctgag gttcaatctg ccagtgcgcc agggtacaac cgctgaaatc
    15601 ctgcgccgcc agccagcttg ctattgcggc attctcctga tttattgtca caattgcgat
    15661 attcatggct gcatcctttg gcgtaaatag tcctgccata gcttttgagc tttttctgct
    15721 accgtttgcc catcaatcag cgtcgctccg cgtcgttgct ctgctcttgc cagttgcaga
    15781 cactgcatcg ctggcatttc ggcagcaaca gttttacgga taatttctgc tttccccgca
    15841 gccattcgct ggcgcattcc cggtactggc aaggccactt caccgcactg acgcacggca
    15901 atcaccgcag gcaagcgaac ccggcaacaa cgcaacccat gttcagtacg ctgttcgagg
    15961 gtgataaaca gcgcgtcgag agtgaaacgc tccacctggg tgaagcaggg ccagcccagc
    16021 atttccgcca gcaaaaatgg cgtttgccca ttctgccctt cgctgctttg gcagccggta
    16081 attatcagat ccagcggatt ctgatgctgc cattcggcaa tatggcgagc gacaaattcc
    16141 ggcgcaaagc gcagatctgc cgccgtctcc agcaatacag cttcctcaaa cccaagagcc
    16201 atgagatagc gcagccagtg caatgcccgt tcatccccca tgctcaacgc ggttaaagac
    16261 atcggcgtac cgttttttct ctgcgccaac agcagcgcgg cagcggcctg ttcatcagca
    16321 ccgagtaaac ttcgcagtag cgaaatatcc ggtccgcttt taccctgagc cgccgcctgc
    16381 cactcttttt ccgccagcat tccggcatcc ggttcggctt taaacgctaa cagaatgttc
    16441 attcgcacct cctacatttc ccgccgccac cagcgggagg gctttagttt ctggtgccca
    16501 tagccaggtc acaacaaaat caaccaacaa cactgtcgcc aggagcaata aggtgacttg
    16561 cattccccac tgcgccagca cccacggcag caggccagta cttaccgccg cgccaagtcg
    16621 actcatggcg gtggcaaaac cgacgcccag cgagcgaatg tcagtaggaa aactttccgc
    16681 aggcaaaatg cccaccagat tactgactgc cgaaatggtg gtgctgaaga gaacaaaaag
    16741 cagcagcgtt aatgaactgc cggaaggcaa acaggccatc actaccagcg ttgccgccag
    16801 cagcaaaaaa cttcccagca aaaatttgcg atgtgccagc aggtgcgtca gaactaatcc
    16861 cagcagcgcg cccacaatta acaacgcatt aagcatcagg ctggcagtca gcgcatcttc
    16921 cagaccaata gtctgggcga tagttggcag ccaggtataa atcacaaacc atgggattac
    16981 gaggcagaca aagaagacgc tgttaaacgc cgtgcgccgc cagtaacgcg aagagaacaa
    17041 ggttttgatg tgtttatggg tcgccgttac cacttcatcg cccagtaaaa catggggacc
    17101 aaaatagcga tgcacgatag cgtgagcttc tgcaaaacgc ccctggcgca gtagccagcg
    17161 tggtgattct ggcgttcccc agcgtaataa cgtaatcaac aacgcgggca gagctgccga
    17221 tgccagtagc cagcgccagg cctccgggtt ttcggaaata aagtgatgtc cggcaatact
    17281 tgccagcaca tagcctacgg tccacaccac gctgaatgcg cccagcaaaa taccgcgatg
    17341 gcggcgcggg gaaaattcag ccagcaaggt gtgacctact gaataatcgc ctcccagacc
    17401 aatgccaatc aaaatgcgca gtccaataag atgctctggc gtggtggcaa aaaattgtaa
    17461 aaacgaagca agcgtaatca gcaaaaagct gaaggtgaag attttttgcc gaccaatatg
    17521 gtcggagatc cacccaagaa ccaggctgcc aaggaacaaa ccaaggagcg ccgagccgcc
    17581 gatcattccc gccataaacg gcgtcagttg catggcgggc gtaagctgaa taatggcgta
    17641 accaatgacg ccgagaacat aaccgtcggt caggtgtgcg ccgaaagtga gcgcagcaat
    17701 gcggcagtga aaacggttaa gcggtaaatc atccattcgc accggtgaag tgttcatctt
    17761 taccccttca ttgtccggta ataagaacgg cttctcgcct gagaagccga aaaggcaacg
    17821 ccccgtcatc gcggaacgct gcctgacaca ttatttttca atcggataga tagtacccgt
    17881 attcataatg ccattaggat cgaactgctt tttcagccct tccagcaacg cccacgcgct
    17941 gccgtgttcc agtttgctcc agtgaacgcg atgtttaccg ataccgtggt ggtgcaccat
    18001 cgaaccgccg aggcgaatgg tttcttcaca gatgatcttg ttgagcggat tgtggtactt
    18061 gtcgatttcc tcttccggct tacagtcaac gacgttgtaa tcgtagacga agtacatgtt
    18121 ggtgccgttc tgatagctat gagaggaatg accgcccagc atggtgatgt cgtcggcgtg
    18181 cgggaactca gtacgaatac ggttaataac gctttcgtag atttcgtgga tgcagctcca
    18241 gcagccggac acttcagtgg taaagcccat gttgccggtt ttgaggatct gcacacgttc
    18301 ggcagccact ttatccggtc cccagttcag gttgttaaac caggtttcga tcagcttgct
    18361 gtccacgcgc tggcattgcg ggtagcgggc aacgatttcc gcaatccctt cgcccgtcgc
    18421 cttcgcaatg cgagggttac cttcagccat aaagatcagc acgcattttc cgtctgcaaa
    18481 atgggtgaag tgttgggtgc catcttcagc gtcatacaaa cgagcgatcg acggacgata
    18541 cccttccacc atgacttcac gcaggatgtt gaagtcggtt ttcatgtctt ccaggatata
    18601 gccgtagaag aggttgtttt ccggggtgaa tttaaagatt ttcactgtta cttcagtgat
    18661 atagcaaagt gcaccttcgt taccgatgat gatgtggcga atgtccgggc cagccgcgcg
    18721 gcgtggcacg tttttaatgc gtgtgacggt gccatcagcc aatactgctt ccagaccaac
    18781 gaccatatct tcgattgcgc cgtagagtgt ggagaactgc ccgatactgc gggttgctac
    18841 caagccgccc atctgcgcca gcggctttga ctgcggagaa tgccccgtgg tgtaaccttt
    18901 ttcacgcaac gcattttcca gcacctccag cggaacccca cattgcgccg tcgcctgcat
    18961 attctcaata tcaatattaa tgatttgatt catggcggag ccgtcaagca ccaccgagtt
    19021 ttccacaacg gtttccagcc caccttcagt ggcggaagcg ccggtacgcg gcacaccgtt
    19081 aattttgtgc gcattcataa aattcagcac acgggatact tgctctgtgg aaccgagttt
    19141 tacgaccgct gccggaatcg gcaaagtata aatgccatga atatccggaa atttacgaaa
    19201 acggtcaata ctgttcttct ttaatactgt ttcatcggta attacgcgat ctgcaccaac
    19261 aatttccttt agttggtcga caatcgctgc gcgagataaa gacatagtaa ttccttcctg
    19321 ataataaaaa tggaataatc attaataaga gaaaacagaa accaccgtta tggattagcg
    19381 cactaaataa ccaccattga ccactaataa atgcccattg acataatttg aagccggact
    19441 tgcgaggaat acagctgcgc ccattaaatc ctgagtatcg ccccaacggt ttgccggaat
    19501 atgatcaaga acgcgctgat tggtttctgg attactgcgt gtcgccagcg taatatctgt
    19561 tgcataatag ccaggagcaa taccatttac ctgaatatta tattgaccta gttcatcaca
    19621 ataagctttg gtgaatccgg caagagcatg tttagtggca gaatatgcag gtgaccattg
    19681 tccacctaag taagagaaca atgaacagat attaataatt ttgccgcttt tctgcgggat
    19741 cataattttt gcagcttcat agcttaactc gaatgcggcg gtcaggttca catcaatcat
    19801 cggatcccag tcggcacgac cgaagtccag caccttattc agcttacaaa taccggcatt
    19861 gtttaccaga atatcaactg taccgaaacg ctcacagcaa gcagcgataa tcttctgcgg
    19921 cgcgccttct gcggtgatat ccacctgcat gaagtccacc tcaacaccct gtttttcaat
    19981 catttccttt gtttcgccgt tatctttcaa tcatttcctt tgtttcgccg ttatctttga
    20041 cgaaactagg aataaagata tttgcgccag ctttggccaa cgccatggca aatgcctggc
    20101 ctaaaccgct attcccaccg gtaacaattg cggttttacc tttcagtgag aaaaaatcca
    20161 ttgagaacgc attgagagat tcgattgaca tagttagctc caatttccgt aaggcaaaaa
    20221 aaagaaaggt aacttccccc gcgagaggga agttaccttt ctcattatct ctggtaactg
    20281 gagataattt ttagcataac ggcataactg ctaatgtgat cgtaatcaca gtgtgatatt
    20341 cgtattgtca aatagtttta aaaagcagaa gcgtgattac tctcacacaa cacctctgct
    20401 ttccatgtta tttcttattg caattactag agacctgaga aaagtaatcc acctgaaagg
    20461 gcggcttact tttctctttt ttttgtctat tacaaatggg aatacgatat gcaacacaac
    20521 tcatatcgcc gttggataac cctcgcgata attagtttta gcggcggcgt tagttttgac
    20581 ctggcttatt tacgttatat ttttcaaatt cccatggcga aatttatggg attcagcaat
    20641 accgagatag gtttaataat gagtaccttt ggtattgcgg ctattattct ttatgccccc
    20701 agcggcgtta ttgccgataa attttcacac cgcaaaatga ttacttccgc gatgatcatt
    20761 accggattac tgggtctgtt aatggcaacg tatccaccgc tgtgggtaat gctctgtatt
    20821 caggtcgcct ttgcgataac gacgatttta atgctgtggt cggtgtcgat taaagccgcc
    20881 tcgttgctgg gcgatcatag tgagcaaggg aaaattatgg gctggatgga agggctgcgc
    20941 ggcgtcggtg taatgtcgct ggcggtgttt accatgtggg tcttttctcg ctttgcaccg
    21001 gatgacagcg ccagcctgaa aacagtcatt atcatctaca gtgtggttta catcttgttg
    21061 gggattctgt gctggttttt tgttagcgat aacaacaacc tgcgcagtgc caataacgaa
    21121 gaaaaacagt cattccagct tagcgacatc cttgccgttt tgcgtatcag caccacctgg
    21181 tattgcagca tggtgatttt tggtgtcttc acaatctacg ccattctgag ttactccacc
    21241 aactatctga ccgaaatgta cggcatgtcg ctggtggcag cgagctacat ggggattgtg
    21301 atcaacaaaa tcttccgcgc gctgtgcggc ccacttggag gcattatcac cacctacagt
    21361 aaagtgaaat cccctacccg cgtgattcaa atcctttcca tcatcggttt gctggcgttg
    21421 acagccctgc tcgtcacgaa ctctaacccc caatcggtcg cgatggggat tggcctgatt
    21481 ttactgctgg gattcacctg ttacgcctca cgcgggctgt actgggcctg ccccggcgaa
    21541 gcgagaacac cgtcttacat tatgggcacc acggtaggta tttgttcggt gattggattc
    21601 ctgccggatg tcttcgttta cccgattatc ggccactggc aagacaccct gcccgctgca
    21661 gaagcctacc gcaatatgtg gctgatgggc atggcggcgc tcggcatggt gattgtcttt
    21721 acctttttgc tgttccaaaa aattcgtact gctgatagcg cccccgcaat agctagcagc
    21781 aagtaagcca ggaaaattac aggagaaaaa tcatgtcgaa aaaatacatc atagggattg
    21841 atggcggaag tcagagcaca aaagtggtga tgtacgatct ggaaggtaat gtggtttgcg
    21901 aaggtaaagg cttattacag ccgatgcaca cgcctgatgc cgatactgca gaacatcctg
    21961 acgacgattt atgggcatca ttatgttttg caggtcacga tttgatgagc cagtttgccg
    22021 ggaataaaga agatattgtt ggcatcggcc tgggctccat ccgttgctgc cgtgcgttat
    22081 tgaaagccga cggcacgcca gctgcgccgt tgattagctg gcaggatgca cgcgttacac
    22141 gcccttacga acatacgaat cctgacgtgg cgtatgtaac ctctttttcg ggttatctga
    22201 cgcatcgctt aaccggcgaa tttaaagata atatcgccaa ctattttggt cagtggccgg
    22261 tggattataa gacctgggca tggagcgaag atgccgcagt catggagaag tttaatatcc
    22321 cccgtcatat gctgtttgat gtgcaaatgc caggcactgt tcttgggcat atcacacctc
    22381 aagccgcgct ggcgacgcat ttcccggcag gactgccggt tgtttgtacc accagtgata
    22441 aaccggtaga agctctggga gccggattac tggatgatga aactgcggta atttctcttg
    22501 gcacttacat cgcattgatg atgaacggca aagccttgcc gaaagatccg gtggcgtact
    22561 ggccgattat gtcttctatt ccgcaaacat tgctgtatga aggttacggt attcgcaaag
    22621 gcatgtggac ggtgagctgg ctgcgcgaca tgttaggcga gtcgttaatt caggatgcca
    22681 gggcgcagga tctttcaccg gaagatttac tcaacaagaa agcctctagt gtgccacctg
    22741 gctgtaatgg gctgatgacg gtgctggact ggctgaccaa tccgtgggaa ccgtacaaac
    22801 gcgggattat gatcggcttt gattccagca tggattacgc atggatatat cgttcgatat
    22861 tggaaagcgt ggcgctgacg ctgaagaaca attacgacaa tatgtgtaat gaaatgaatc
    22921 actttgcgaa acatgtaatc attactggcg gcggttcgaa caggatctgt ttatgcagat
    22981 ttttgccgac gtgttcaacc ttccggcacg ccgtaacgcc attaacggtt gtgcaagcct
    23041 gggggcagcg attaatacag gggtaggtct ggggctatac ccggattacg ca
//
LOCUS       DAJVRF010000059        22703 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJVRF010000059 DAJVRF010000000
VERSION     DAJVRF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22703
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.059"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="OQN13_002075"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="OQN13_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCS1615433.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="OQN13_002076"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="OQN13_002076"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCS1615434.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="OQN13_002077"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="OQN13_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCS1615435.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="OQN13_002078"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="OQN13_002078"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCS1615436.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="OQN13_002079"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="OQN13_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCS1615437.1"
                     /translation="MELLVLVWRQYRWPFISVMELSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6233..7879
                     /gene="mqo"
                     /locus_tag="OQN13_002080"
     CDS             6233..7879
                     /gene="mqo"
                     /locus_tag="OQN13_002080"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCS1615438.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8188..8676)
                     /gene="eco"
                     /locus_tag="OQN13_002081"
     CDS             complement(8188..8676)
                     /gene="eco"
                     /locus_tag="OQN13_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCS1615439.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            9084..9578
                     /gene="napF"
                     /locus_tag="OQN13_002082"
     CDS             9084..9578
                     /gene="napF"
                     /locus_tag="OQN13_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCS1615440.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            9568..9831
                     /gene="napD"
                     /locus_tag="OQN13_002083"
     CDS             9568..9831
                     /gene="napD"
                     /locus_tag="OQN13_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCS1615441.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            9828..12314
                     /gene="napA"
                     /locus_tag="OQN13_002084"
     CDS             9828..12314
                     /gene="napA"
                     /locus_tag="OQN13_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCS1615442.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            12321..13016
                     /gene="napG"
                     /locus_tag="OQN13_002085"
     CDS             12321..13016
                     /gene="napG"
                     /locus_tag="OQN13_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCS1615443.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            13003..13866
                     /gene="napH"
                     /locus_tag="OQN13_002086"
     CDS             13003..13866
                     /gene="napH"
                     /locus_tag="OQN13_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCS1615444.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            13863..14312
                     /gene="napB"
                     /locus_tag="OQN13_002087"
     CDS             13863..14312
                     /gene="napB"
                     /locus_tag="OQN13_002087"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCS1615445.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            14322..14924
                     /gene="napC"
                     /locus_tag="OQN13_002088"
     CDS             14322..14924
                     /gene="napC"
                     /locus_tag="OQN13_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCS1615446.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            14943..15560
                     /gene="ccmA"
                     /locus_tag="OQN13_002089"
     CDS             14943..15560
                     /gene="ccmA"
                     /locus_tag="OQN13_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCS1615447.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            15557..16219
                     /gene="ccmB"
                     /locus_tag="OQN13_002090"
     CDS             15557..16219
                     /gene="ccmB"
                     /locus_tag="OQN13_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCS1615448.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            16261..16998
                     /gene="ccmC"
                     /locus_tag="OQN13_002091"
     CDS             16261..16998
                     /gene="ccmC"
                     /locus_tag="OQN13_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCS1615449.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            16995..17204
                     /gene="ccmD"
                     /locus_tag="OQN13_002092"
     CDS             16995..17204
                     /gene="ccmD"
                     /locus_tag="OQN13_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCS1615450.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            17201..17680
                     /gene="ccmE"
                     /locus_tag="OQN13_002093"
     CDS             17201..17680
                     /gene="ccmE"
                     /locus_tag="OQN13_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCS1615451.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            17677..19620
                     /gene="ccmF"
                     /locus_tag="OQN13_002094"
     CDS             17677..19620
                     /gene="ccmF"
                     /locus_tag="OQN13_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCS1615452.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSIDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            19617..20174
                     /gene="dsbE"
                     /locus_tag="OQN13_002095"
     CDS             19617..20174
                     /gene="dsbE"
                     /locus_tag="OQN13_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCS1615453.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            20171..21223
                     /gene="ccmH"
                     /locus_tag="OQN13_002096"
     CDS             20171..21223
                     /gene="ccmH"
                     /locus_tag="OQN13_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCS1615454.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(21258..21905)
                     /gene="narP"
                     /locus_tag="OQN13_002097"
     CDS             complement(21258..21905)
                     /gene="narP"
                     /locus_tag="OQN13_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCS1615455.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            22225..22596
                     /locus_tag="OQN13_002098"
                     /pseudo
     CDS             22225..22596
                     /locus_tag="OQN13_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         5089 a         5689 c         6450 g         5475 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaat atctatagcg ataaatgaaa catcttaaaa gttttagtat catattcgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatgga gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca ggtcaatatt
     6061 ctaaaaatcc cttcatttct aataacccta taattaatca acgaaattat aatgtttcta
     6121 aaattagaat ataatttata aacattattt aaatgttgtt actttagtgt taccgttgat
     6181 gccgcgcaaa tctcacctgc aataaagcga ctaaaagtaa ggcattaaca agatgaaaaa
     6241 agtgactgcc atgctcttct cgatggccgt ggggcttaat gccgtttcga tggcggcaaa
     6301 agcgaaagcg tccgaggagc aggaaactga tgtactgttg attggcggcg gcattatgag
     6361 cgccacgttg gggacctatt tacgcgagct ggagcctgaa tggtcgatga ccatggtgga
     6421 gcgcctggag ggtgtcgcgc aggagagttc gaacggctgg aacaacgccg gaaccgggca
     6481 ttctgcactg atggaactga actacacccc gcaaaacgcc gatggcagta tcagtattga
     6541 aaaagcagtc gccattaacg aagcatttca gatttcccgc cagttctggg cgcaccaggt
     6601 cgagcgcggc gtgctgcgta ctccgcgttc atttatcaat accgttccgc atatgagctt
     6661 tgtctggggc gaggataacg ttaatttcct gcgcgcccgt tacgccgcgt tgcaacaaag
     6721 ctcgctgttt cgcggtatgc gttactctga agatcacgcg cagatcaaag agtgggcacc
     6781 gttagtgatg gaagggcgcg atccgcaaca gaaagtggca gccacgcgta cggaaattgg
     6841 taccgatgtg aactacggcg agatcacccg ccagttaatt gcttccttgc agaagaaatc
     6901 taacttctcg ctgcaactca gcagcgaagt ccgcgcccta aagcgtaatg acgataacac
     6961 ctggaccgtt accgttgccg atctgaaaaa tggcactgca cagaacattc gtgccaaatt
     7021 tgtctttatc ggcgcgggcg gtgcggcgct gaagctgtta caggaatcgg ggattccgga
     7081 agcgaaagac tacgccggtt tcccggtggg cggacagttc cttgtttcgg aaaacccgga
     7141 cgtggttaat caccatctgg cgaaggttta cggtaaagca tccgttggcg caccaccgat
     7201 gtcggttccg catatcgata cccgcgttct ggacggtaaa cgcgtagtgc tgtttgggcc
     7261 atttgccacc ttctcaacca aattcctcaa aaacggttca ttgtgggatc tgatgagttc
     7321 caccaccacc tctaacgtga tgccgatgat gcacgtcggg ctggataatt tcgatctggt
     7381 gaaatacttg gtgagtcagg tgatgttgag tgaagaggat cgttttgaag cgttgaaaga
     7441 gtactatccg caggcgaaaa aagaggactg gcgtttgtgg caagcggggc agcgtgtgca
     7501 gattatcaag cgtgatgccg ataaaggtgg tgtactgcgt ctgggtactg aagtggtcag
     7561 tgaccagcaa gggaccattg ccgcgctgct gggggcatcg ccaggggcgt caaccgctgc
     7621 gccgattatg ttggatctgc tggaaaaagt atttggcgat cgcgtttcca gcccgcaatg
     7681 gcaggctacg ttgaaagcga tcgttccatc ttatggacgc aagctgaacg gtgatgtagc
     7741 ggcaacagaa cgcgagttgc agtacaccag cgaagtgctg ggcctgaaat acgacaagcc
     7801 gcaggcagca gatagtacgc cgaaaccgca gttgaaaccg caacccgttc aaaaagaagt
     7861 ggcggatatt gcgttgtaat gatgtgccac atccggcatg gtatgccgga tgtggcgtaa
     7921 ccgccttatc cgacctacgg ggtggagcac ttcacagcgg tgtaggcctg ataagacgcg
     7981 tcagcgtcgc atcaggcaac cggctcgggc gttagatgtc ggatgcggcg taaccgcctt
     8041 atccaaccta cggggtggag cacttcacag cggtgtaggc ctgataagac gcgccagcgt
     8101 cgcatcaggc aaccggcttg ggcgttagat gtcggatgcg gcgtgaccgc cttatccgac
     8161 ctacggggtg acgcacttca cagcagttta gcgaactacc gcgttgtcaa ttttctcttc
     8221 cgccttccag acgcggtact tcacatctac attgtctggc gtatacacca cgatcggcag
     8281 cttgctgttg taacgcagca ttccagcatc gcccagatac gcggtgacaa atttcttctc
     8341 tttcttgcca tccgggcagg ccatcatcgt ggaaaccggc gagctaactt tatcaaagac
     8401 ataataatca tagccccagc cttccagcgt tttgttttcc agcttcccgc cgagacgatg
     8461 caaattgcaa tcgacttcca gcgtctgacc ggttaacagt tctactttca gggtagattc
     8521 atcttcttgc ggggttaact gaatcacctg acgcttcatc cctttttcag cttgtggata
     8581 aggcgcgatt ttttccagtg gctggacgct ttctgccgcc caggcggaag tggtggcgaa
     8641 agcggcaaac aatactgcag gtagaatggt cttcattttt ttgctttcct tcttcattta
     8701 taggttttca ccgatgatgt cgctgaaggt taacatattc atcagattta ttaatttgct
     8761 ttatgaataa cttaaagaac attcagacaa ttttacctgc tcttttagtg ttaaattctg
     8821 atgagggagc aaaccagcag aaatatctct ggtgaattaa tcgctatata aatatgttta
     8881 taaccatttg aaatgtgagc aaaagcccgt ttttgccacg cttcccgcca ctcttttgat
     8941 cctgctacag gttttacccc gatcggggta tgcatctttg acacatcctt taatatctta
     9001 gcggctataa aaatggctta ttaattatgc ggtttatttg gtcgctctca attttcagag
     9061 cgcgttaatg atggaaggtc aatgtgaaga ttgatgcatc ccgtcggggc atactcactg
     9121 gtcgctggcg caaagccagt aacggtatcc gtccgccctg gtccggtgat gaatctcatt
     9181 ttctgaccca ttgcacccgt tgcgacgcct gtattaatgc ttgtgaaaac aacattctgc
     9241 aacgcggcgc gggcggctat ccgagcgtta atttcaaaaa taacgagtgc agcttctgtt
     9301 acgcctgcgc gcaggcttgc cccgaatcgc tattttctcc gcgccacacc agggcctggg
     9361 atttgcagtt caccattggg gacgcgtgcc tggcgtatca gtcagttgaa tgccgccgct
     9421 gtcaggatag ctgtgagcca atggcaatta tctttcgccc gacgctctcc gggatctacc
     9481 agccgcaact taatagccaa ctttgcaacg gatgtggagc atgtgcagcc agctgtccgg
     9541 tatcagccat aactgcggag tatctccatg cacactaact ggcaagtttg cagcctggtc
     9601 gtgcaggcca aaagcgaacg aatttcagac atcagcaccc aactgaacgc ctttcccggc
     9661 tgtgaagttg ctgtcagcga cgcgccgagc ggtcagttga ttgtggtggt ggaagcagaa
     9721 gacagcgaaa cgctgatcca aaccattgag tcagtacgca acgtagaggg cgtgctggcg
     9781 gtgtcgctgg tttatcacca gcaggaagag caaggtgagg aaacaccatg aaactcagtc
     9841 gtcgtagctt tatgaaagct aacgccgttg cggccgctgc ggcggctgcc ggtctcagcg
     9901 tgccgggcgt tgcccgcgcc gttgttggtc agcaggaagc cattaaatgg gataaagcgc
     9961 cgtgccgttt ctgcggtact ggttgcggcg ttctggtcgg aacgcagcag gggcgtgtgg
    10021 tggcctgtca gggcgacccg gacgcaccgg ttaaccgtgg cctgaactgc attaagggct
    10081 atttcctgcc caagatcatg tacggtaaag accgtttgac gcagccgctg ctgcgtatga
    10141 aaaacggtaa atatgacaaa gaaggcgaat ttaccccaat cacctgggat caggccttcg
    10201 atgtgatgga agagaaattc aaaaccgccc tgaaagaaaa agggccggaa tcgatcggta
    10261 tgttcggttc tggtcagtgg actatctggg aaggttatgc cgcgtccaag ctgtttaaag
    10321 cgggcttccg ttcgaacaac atcgacccga acgcgcgtca ctgtatggcg tcggcagtag
    10381 ttggctttat gcgtaccttt ggtatggatg agccgatggg ctgctatgac gacatcgagc
    10441 aggctgacgc gtttgtgctg tggggcgcaa acatggcgga gatgcacccg atcctctggt
    10501 cgcgcatcac taaccgtcgt ctctctaatc agaacgtcac cgtggcggtg ctttctacct
    10561 accagcatcg tagcttcgag ctggcggata acggcatcat ctttacgcca cagtctgacc
    10621 tggtgatcct gaactacatc gccaactata tcattcaaaa caatgcgata aatcaggact
    10681 tcttcagcaa acacgttaac ctgcgcaaag gggcgacgga catcggctac ggtttacgtc
    10741 cgacccatcc gctggaaaaa gcagcgaaga atccaggttc tgacgcctcc gaaccgatga
    10801 gctttgaaga ttacaaagcc ttcgttgccg agtatacgct ggaaaaaacc gctgaaatga
    10861 ccggcgtgcc gaaagaccag ttagaacaac tggcgcagct gtatgccgat ccgaacaaga
    10921 aagtcatctc ctactggacg atgggcttca accagcatac tcgtggcgtg tgggctaaca
    10981 acctggtcta caacctgcac ctgctgaccg gcaaaatttc ccagccgggt tgcggtccgt
    11041 tctccctgac cgggcaacct tctgcgtgtg gtactgcgcg tgaagtgggc acctttgctc
    11101 accgtctgcc tgcggacatg gtggtgacca acgagaaaca ccgtgatatc tgcgagaaga
    11161 agtggaatat cccgagcggc accattccgg cgaaaatcgg tctgcacgcg gtggcacaag
    11221 accgtgcgct gaaagacggc aaactgaatg tttactggac catgtgtacc aacaacatgc
    11281 aggccgggcc gaacatcaat gaagagcgta tgccgggctg gcgcgatccg cgcaacttca
    11341 tcatcgtctc cgatccgtat ccgacagtca gtgcgctggc cgccgacttg atcctgccga
    11401 ccgcaatgtg ggtagagaaa gagggtgctt acggtaacgc cgaacgccgt actcagttct
    11461 ggcgtcagca ggtacaggca ccgggcgaag cgaaatcgga tctctggcag ttagtccagt
    11521 tctcccgccg cttcaaaact gaagaagtgt ggccggaaga gctgctagcg aagaaaccgg
    11581 aactgcgtgg caaaacgctg tacgaagtgc tttatgccac accggaagtg agcaaattcc
    11641 cggtatccga actggcggaa gatcagctga acgatgaatc ccgcgagctg ggcttctatc
    11701 tgcaaaaagg gctgttcgaa gagtacgcat ggtttggtcg cggtcacggt cacgatctcg
    11761 cgccgttcga tgactaccac aaagcgcgcg gtctgcgctg gccggtggtg aacggtaaag
    11821 aaacgcagtg gcgttacagc gaaggtaacg acccgtacgt gaaagcgggc gaaggctata
    11881 agttctacgg taaaccggat ggtaaagcgg tgatcttcgc gctgccgttc gaaccggcgg
    11941 cggaagcacc ggatgaagag tacgacctgt ggctctctac cggacgcgtt ctggagcact
    12001 ggcacactgg cagtatgact cgccgtgtgc cggaactgca ccgcgccttc ccggaagcgg
    12061 tcttgtttat tcacccgctg gatgcgaaag cgcgcgatct gcgccgtggc gacaaagtga
    12121 aagtggtttc tcgccgtggc gaagtgatct cgattgttga aacgcgcggt cgtaaccgtc
    12181 cgccacaggg cctggtgtac atgccgttct tcgacgccgc acagctggtt aacaaactaa
    12241 cgctggacgc gaccgatccg ctctcgaaag agacggactt caagaagtgc gcagtcaaac
    12301 tggagaaggt gtaagccatt atgtcccggt cagcgaaacc tcaaaatggt cgccgccgct
    12361 ttctgcgcga tgttgttcgc acagcaggcg ggctggctgc cgtgggtgtg gcgctggggt
    12421 tacaacagca aaccgcacgc gcatctggcg tgcggttgcg cccgcccgga gccataaacg
    12481 agaacgcctt tgccagtgcc tgtgtgcgtt gcggtcagtg tgttcaggct tgcccttacg
    12541 acaccttaaa actggcgacg ctggcttctg gcctgtcggc gggcacgcca tattttgtcg
    12601 cacgggatat tccttgcgaa atgtgtgagg acattccgtg cgccaaagtg tgcccgagtg
    12661 gtgcgctgga tcgtgagatc gaatcgatcg acgacgcgcg gatggggctg gcggtgctgg
    12721 tggaccagga aaactgtctc aactttcagg ggctgcgctg cgatgtttgt tatcgcgaat
    12781 gcccgaaaat tgatgaggcc atcaccctgg agctggagcg caacacgcgt accggtaagc
    12841 acgcccgctt tctgccgacg gttcacagcg acgcctgtac cggctgcggt aagtgcgaaa
    12901 aagtgtgcgt gctggaacaa ccggcaatca aggtgttacc gctgtcactg gcgaaagggg
    12961 agttaggtca ccattaccgc ttcggctggc tggaggggaa caatggcaaa tcgtaaacgt
    13021 gacgccgggc gcgaggcgct ggtgaaaaaa ggctggtggc gcagtcaccg ttggctggtg
    13081 ttacgtcgcc tttgtcagtt cttcgtgctg gggatgtttt tgagcggtcc gtggtttggt
    13141 gtgtggatct tacacggcaa ctacagctgt agcctgttat tcgacaccgt tccgctgacc
    13201 gatccgctga tgacgctgca aagtcttgcc agtggtcatc tgcctgccac ggtggcgttg
    13261 accggtgcgg tgataatcac tgtgctctac gccctggcag gaaagcgatt attttgcagc
    13321 tgggtctgcc cgctgaaccc gattaccgac ttagcgaact ggttacgcag gcgctttgac
    13381 ctcaatcagt ctgcgacgat ccctcgccac attcgctacg tgctgttggt ggtcattctg
    13441 ctgggatcag ccttaaccgg cacgctcatc tgggaatgga tcaaccccgt ttccctgatg
    13501 gggcgtagcc tggtaatggg cttcggcagc ggcgcgctgc ttattctcgc gctgttttta
    13561 tttgatctac tggtcgttga acacggctgg tgcgggcaca tttgtccggt tggcgcgctg
    13621 tatggcgtgc tgggtagcaa aggtgtgatt accgttgcgg ccagcgatcg gcagaaatgt
    13681 aaccgctgta tggattgttt tcatgtttgc ccggaaccgc atgtgctacg tgccccggtg
    13741 ctggatgaac aaagcccggt gcaggtcacc agccgcgatt gcatgacgtg cggtcgctgc
    13801 gtggatgtct gttctgagga tgtatttaca ataactacac gatggagttc gggagcgaaa
    13861 tcatgaaaag ccatgacctg aagaaagcgc tgtgtcaatg gacggcgatg ctggccctgg
    13921 tggtaagcgg cgcggtttgg gcggctaatg gcgtcgattt tagccagtcg ccggaagtat
    13981 ccgggacgca ggaaggggcc attcggatgc caaaagagca ggatcggatg ccgctgaact
    14041 atgtgaatca gccgccaatg atcccgcata gcgttgaagg ttatcaggta acgaccaata
    14101 ccaaccgctg cttgcagtgc cacggtgtcg aaagctatcg caccactggc gcgccgcgta
    14161 tcagtcctac tcactttatg gacagcgacg gcaaagtggg cgcggaagtg gctccgcgtc
    14221 gttatttctg tctgcaatgt cacgtaccgc aggccgatac cgcgccaatc gtggggaata
    14281 cctttacccc atcaaaaggt tacgggaaat aagaggttat tatgggaaat tctgaccgta
    14341 agcctggtct gattaagcgc ctgtggaaat ggtggcgtac ccccagccgt ctggcgctgg
    14401 gaacgctgct gttgatcggt tttgtcggcg gcatcgtctt ctggggcggc tttaacaccg
    14461 gaatggaaaa agccaatacc gaagagttct gcattagctg ccacgaaatg cgtaacacgg
    14521 tgtatcagga atacatggat tccgtgcact acaacaaccg tagcggcgtc cgtgcgacct
    14581 gtccggattg tcacgttccg cacgagtttg tgccgaagat gatacgcaag ctcaaagcaa
    14641 gtaaagagct gtatggtaaa atttttggcg ttattgacac gccgcagaaa tttgaagctc
    14701 atcgtctgac aatggcacag aatgagtggc ggcgcatgaa ggacaataac tcgcaggagt
    14761 gccgtaactg tcacaacttc gagtatatgg atacaaccgc ccagaaatcg gttgccgcga
    14821 agatgcatga ccaggcggtg aaagatgggc aaacctgtat tgattgccat aaagggatag
    14881 cgcacaagct gcccgatatg cgtgaagtcg agccaggttt ttaacagggt tattgcgtgg
    14941 gtatgcttga agccagagag ttactttgtg agcgggatga acgaacctta tttagtggct
    15001 tgtcatttac gctgaacgca ggagagtggg tacaaatcac cggtagcaac ggcgcgggga
    15061 agacaacgct tctccgtttg ctgacggggt tgtctcgccc tgacgcaggc gaggttctct
    15121 ggcaagggca gcccttgcat caggtacgcg acagctacca tcaaaacctg ttatggatag
    15181 gccatcagcc ggggatcaaa acccggctga cggcgttaga aaatctgcac ttttatcatc
    15241 gcgatggcga taccgcacaa tgtctggaag ccctggcgca ggccgggctt gccggattcg
    15301 acgatattcc ggtaaatcaa ctttctgccg ggcaacaacg ccgcgtcgct ttagcgcgtc
    15361 tgtggctgac ccgtgccacg ttatggatcc tcgacgagcc ttttaccgcg attgacgtta
    15421 acggcgtcga tcgtctgacc cagcgtatgg cgcagcatac ggagcagggg gggattgtga
    15481 ttctgactac ccaccagccg ctcaacgttg ctgaaagtaa aattcgccgc atttcactga
    15541 cgcaaacgag ggccgcatga tgttctggcg cattttccgt cttgagctgc gtgtagcgtt
    15601 tcgccatagc gccgaaatcg ccaacccgct gtggttcttc ctgattgtaa ttaccctttt
    15661 tccgctcagt atcggtccgg agccgcaact gctggcgcgt attgcaccgg gcattatctg
    15721 ggttgctgcg ctgctttcat ccttgctggc gctggaacga ctgttccgtg acgatttgca
    15781 ggacggcagt cttgaacaat tgatgttgtt gccgttaccc ttgcccgccg ttgtgctggc
    15841 gaaggtgatg gcgcactgga tggtaaccgg tctgccgtta ctcatccttt cgccactggt
    15901 agcaatgcta ctgggaatgg atgtttatgg ctggcaagtg atggcgctga cgctgctgct
    15961 gggaacgcct acgcttggct ttctcggtgc accgggtgtg gcgttgacag tgggacttaa
    16021 gcgcggtggt gtgctgctca gcatactggt gttaccgctg actatcccat tactcatctt
    16081 tgccaccgcc gcgatggacg cggcttccat gcatttgccc gttgacgggt atctggcaat
    16141 tttaggcgcg ttgctggcag gcaccgcgac attaagtcct tttgcgacgg cggcagcgtt
    16201 acgaatcagc attcaataac gctggattac tgatttttgt tcgagtctgg tatcgaaact
    16261 atgtggaaaa cactgcatca actggcgatc ccaccacggc tgtatcaaat ctgtggctgg
    16321 tttataccgt ggctggcaat tgccagtgtg gtcgtgctta ccgtcggctg gatctgggga
    16381 ttcggctttg ctccggctga ttatcagcag ggaaatagct accgcattat ctacctgcat
    16441 gtgcctgcgg cgatctggtc gatgggcatt tatgcatcaa tggcagtggc agcgtttatt
    16501 ggccttgtct ggcagatgaa aatggccaac ctggcggtgg cggcgatggc ccccattggt
    16561 gccgtgttta cctttattgc cctggttacc ggctctgcat ggggaaaacc gatgtggggc
    16621 acctggtggg tatgggatgc acgtctgact tctgaactgg tgctgctgtt tttgtatgtg
    16681 ggtgtgattg ccctgtggca cgccttcgac gaccgccgtc tggcgggccg tgcggcaggt
    16741 atcctggtgc tgattggcgt ggtgaatctg ccgattattc attactccgt ggagtggtgg
    16801 aacaccctgc atcagggatc aacgcggatg cagcaaagta tcgatccggc gatgcgttcg
    16861 ccgctgcgct ggtcgatttt tggcttcctg ctcctgtctg ccacgctgac gctgatgcgg
    16921 atgcgtaatt tgattttgct gatggaaaaa cgccgtccgt gggtgagtga actgatactg
    16981 aaaagaggcc gtaaatgacc cctgcatttg cttcctggaa tgaatttttc gcaatgggcg
    17041 gttacgcctt ttttgtctgg ctggcggtgg tgatgaccgt tattccgctg gtggttttgg
    17101 tcgtgcactc ggtgatgcaa catcgcgcaa ttctgcgtgg tgtggcgcaa cagcgggcgc
    17161 gtgaggcgcg tttacgtgct gcgcaacagc aggaggctgc atgaatattc gccgtaaaaa
    17221 ccgcttgtgg attgcctgtg ccgtgttggc agggctggcg ctgactatcg gtctggtgct
    17281 atatgcgctg cgctcgaata tcgatctctt ttatacgccg ggggaaattc tctacggcaa
    17341 gcgtgaaact cagcaaatgc cggaagtcgg tcagcgtttg cgcgttggcg ggatggtgat
    17401 gctgggtagt gtgcagcgcg atcccaattc gctgaaagtg accttcacta tttacgatgc
    17461 tgaaggctca gtggatgtct cttacgaagg cattttgccg gatctgttcc gtgaagggca
    17521 gggcgttgtg gtgcagggcg agctggaaaa aggcaatcat atcctcgcga aagaagtgct
    17581 ggcgaaacac gacgaaaact acacgccgcc agaagttgag aaagcgatgg aagctaacca
    17641 ccgtcgcccg gcgagtgttt ataaggaccc agcatcatga tgccggaaat tggaaacggg
    17701 ctgctgtgtc tggcgttagg gattgcgctg ctgctgtccg tgtatccgct atggggcgtg
    17761 gcgcgcggag atgcgcgcat gatcgcgtct tcccgcttgt ttgcctggct gctgtttatg
    17821 tctgtggctg gcgcatttct ggtgctggtc aatgctttcg tggtcaacga ctttaccgtc
    17881 acctatgttg ccagctactc caatacccag cttccggtgt ggtatcgcgt ggcggctacc
    17941 tggggcgcgc atgaaggctc gctactgctg tgggtgctgc tgatgagcgg ctggaccttt
    18001 gcggtggcga tttttagtca gcgtattccg ctggatattg tggcccgcgt actggcgata
    18061 atggggatgg tcagcgtcgg ctttttgctg ttcattctct ttacctctaa cccattctca
    18121 cgcacgctgc cgaacttccc gattgaaggg cgcgatctta acccgctgtt acaggacccg
    18181 gggctgatct tccatccgcc tctgctttat atggggtacg tgggtttctc ggtggcgttt
    18241 gcttttgcca ttgcttcttt gctgagcggg cgtctggaca gcacttatgc gcgttttact
    18301 cgcccgtgga cgctggcggc gtggattttc ctgacgctcg gcattgtgct cggttccgca
    18361 tgggcctatt acgaactcgg ctgggggggc tggtggttct gggatccggt agaaaacgcc
    18421 tcgtttatgc cgtggctggt ggggactgcg ctgatgcact cactggcggt cactgaacaa
    18481 cgcgccagct tcaaagcgtg gacattactg ctggcaatca gtgccttctc gttgtgtctg
    18541 ttggggactt tcctcgtgcg ttccggcgtg ctggtatcgg tacacgcgtt tgcgtctgat
    18601 ccggcgcgcg gtatgtttat cctcgccttt atggtgctgg tgattggagg ttcgctgctg
    18661 ctgtttgccg cgcgtggaca caaagttcgc tcacgcgtaa acaatgcgct gtggtcgcgg
    18721 gaatctctgc tgttagcgaa caacgttttg ctggtcgccg cgatgctggt ggtgttgctg
    18781 gggacgctgc tgccgctggt gcacaagcaa ctgggactgg gcagtatttc gattggcgaa
    18841 ccgttcttca acaccatgtt tacctggctg atggtgccgt ttgcgctact gctgggtgtc
    18901 ggtcctctgg tgcgctgggg gcgcgatcgc ccacgtaaaa tccgcaattt attgattatc
    18961 gccttcatct caacgctggt gctgtctctg ctgttgccgt ggctgtttga gagcaaagtt
    19021 gtggcgatga cggtgctcgg cctggcaatg gcctgctgga ttgcggtgct ggcaattgcg
    19081 gaagctgcgc tgcgtatttc acgcggcacg aaaaccacct tcagttattg ggggatggtg
    19141 gctgctcacc tggggctggc agtgacaatt gttggcattg cttttagcca gaactatagc
    19201 gttgagcgtg atgtgcgcat gaagtccggc gatagcatcg atattcatga atatcgcttc
    19261 accttccgtg atgtcaaaga ggtgactggc ccgaactggc gtggcggtgt ggcgactatc
    19321 ggcgtaacgc gagagggtaa accggaaaca gtgctgtatg cggaaaaacg ttattacaac
    19381 actgccgggt cgatgatgac cgaagcggcg attgacggtg gcatcacgcg tgacctgtac
    19441 gccgcgctcg gtgaagagct ggaaaacggc gcgtgggctg tgcgtcttta ctacaaacca
    19501 tttgttcgct ggatttgggc gggcgggctg atgatggcgt tgggcggact gctgtgtctg
    19561 tttgatcctc gctatcgtaa gcgcgtgagt ccgcaaaaaa ctgcgccgga ggctgtatga
    19621 agcgcaaagt attgttaatt ccgttgatta tcttcctggc gattgccgcg gcgctgctgt
    19681 ggcagctggc gcgtaatgcc gaaggggatg atccgaccaa tctggaatcg gcgctcattg
    19741 gcaagccagt gccgaagttt cgcctcgaat cactggacaa tccggggcag ttttatcagg
    19801 cggatgtgct gactcagggc aaaccagtgc tgcttaacgt ctgggcgacc tggtgtccga
    19861 cctgccgtgc ggaacatcaa tatctgaatc agctttctgc gcagggcatc cgcgtagtcg
    19921 gcatgaacta taaagacgat cgccagaagg caatcagctg gctgaaagag ctgggcaatc
    19981 cttacgcgct aagcctgttt gatggcgatg gcatgttagg gctggatctc ggtgtctatg
    20041 gcgcgccaga aacgttcctt attgacggca acggcatcat tcgctatcgc catgcgggcg
    20101 atctcaatcc tcgcgtctgg gaagaagaga tcaagccgct gtgggagaaa tacagtaagg
    20161 aggccgcaca atgaggtttt tattgggcat gctgatgctg atgatctccg gctcagcgct
    20221 ggcgaccata gacgtgttgc agtttaaaga tgaagcacag gaacaacagt tccgtcagct
    20281 cactgaagaa ctgcgctgcc cgaaatgcca gaacaacagc attgccgatt ccaactcgat
    20341 gattgccacc gacctgcgcc agaaagtgta tgaactgatg caggaaggta aaagtaaaaa
    20401 agagattgtc gattatatgg tggcgcgtta cggcaacttc gtcacttacg atccgccgtt
    20461 aacgccgctg accgtgctgc tgtgggtgct gccagtagta gctattggca ttggcggttg
    20521 ggtcatatac gcccgctcgc ggcgtcgggt acgcgtggtg ccggaagcgt ttcctgaaca
    20581 aagcgtgccg gaaggtaagc gtgccggata tgtcgcttat ttgccgggca ttgtggtggc
    20641 gttaattgtg gctggcgtca gctactacca gaccggaaat tatcaacagg tgaaaatctg
    20701 gcagcaggcc acggcacagg ctccggcgtt gctggacagg gcgctggatc cgaaagctga
    20761 tccgctcaac gaagaagaga tgtcgcgcct tgcgctgggg atgcgtactc aactgcaaaa
    20821 aaatccggga gatatagacg gctggattat gttgggccgc gttggcatgg cgctgggtaa
    20881 cgccagtatc gccaccgatg catacgctac tgcgtatcgc ctcgatccga aaaacagtga
    20941 tgccgcactg ggttatgctg aagcgttgac acgttcatct gatcccaacg acaaccgcct
    21001 cggcggcgaa ctgctacgtc agctggtgag aacggaccat agcaatatcc gtgtgctaag
    21061 catgtatgcg tttaatgcct ttgagcagca gcgatttggc gaagccgttg ccgcgtggga
    21121 gatgatgttg aaactcttac ctgccaacga tactcgccgt gcggtgattg aacgtagtat
    21181 cgcgcaggcg atgcaacatt tgtcgccgca ggagagtaaa taaaaagagg cccgatggtg
    21241 atgccatcgg gctattttta ttgtgccccg cgttgttgca ggaacagaat ggtggccgcc
    21301 acgcgtgagc ggacattgag tttacgcagc agatttcgaa tatgaacttt tactgtctgc
    21361 tcggaaatat tcaacacaga ggcaatctgt ttatttgaca gcccctgtgc cagctcgtgc
    21421 agaacatcca gctcgcgctc cgtcagcacg ctgaagggat cttcttccgc gccaaacatt
    21481 tcacgttcac gtaagtactg attgacgcgt tcgctaaaga ctttgctgcc tttcgctccg
    21541 gcacgaatcg cttccagcaa cacttccggg tcgctgtctt tcagcagata accgtctgcg
    21601 cctgcgtcta tcagcgcaaa gacatcgctg gaggcatcgg atacggtcag gataataatt
    21661 tgcgcggtaa cgccatccct gcgcaaggca ttgagggtat ccaggccact catacctttc
    21721 atattgagat ccagcaagat cacgtcgata tccagtctat tcgccagatc gatcgcgctc
    21781 gcgccgtcgc ccgcttcggc gaccacttca aagccaggat caagctccag taactgacga
    21841 acaccgcgtc gcataagtgg atgatcatcc acaatcatca cctgaaaagg cgttgcttca
    21901 ggcatagtag tctcctgagg ttttattaga atcattatgt ttgagcgtag cgttggtaac
    21961 gctaccaggc gcgtgaatgt taccccattt tatgtatgga catgtagtga aaactacaac
    22021 cttgccgttc gctttggcat cattttcatt ataaagacgg aattttgcat cagcttctca
    22081 tcaagaaaaa caactaaaaa atcaatcaca atacttgtgc atttctttaa acagttttta
    22141 aaaactgact ccgggtatgg agctatgggt attttctgta cccaatgctt ttaacagcaa
    22201 ttaatttcat aggatgaaag ctcaatgcat caatctggtt ctgtttctct ttgtcgttcc
    22261 gcaatatctg ttctggtggc tacagcgtta tattcaccca tagcattggc atcaacagtt
    22321 gagtatggtg agacagttga tggtgttggc ctggaaaaag atatccagct ggtttatggg
    22381 accgccaata atacgaaaat caatcctggc ggagaacagc atataaaaga atttggtgta
    22441 agtagtaata ctgaaattaa cggcgggtat cagtacattg aaatgaatgg cactgcagaa
    22501 tactcagtat taaatgatgg ttatcaaatt gttcaaatgg gtggcgcggt aaaccagact
    22561 acgctcaata atggagtgct acaggtttat ggcgctagac tggccccctg aatctccaga
    22621 caaccagtat cacttattta agtgatagtc ttaatactag tttttagact agtcattgga
    22681 gagcagatga ttgatgtctt agg
//
LOCUS       DAJVRF010000060        22122 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJVRF010000060 DAJVRF010000000
VERSION     DAJVRF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22122
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.060"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..525)
                     /locus_tag="OQN13_002099"
     CDS             complement(<1..525)
                     /locus_tag="OQN13_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS1615456.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQR"
     gene            821..1597
                     /gene="exuR"
                     /locus_tag="OQN13_002100"
     CDS             821..1597
                     /gene="exuR"
                     /locus_tag="OQN13_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCS1615457.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1942..2604
                     /locus_tag="OQN13_002101"
     CDS             1942..2604
                     /locus_tag="OQN13_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1615458.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            2608..2991
                     /gene="mzrA"
                     /locus_tag="OQN13_002102"
     CDS             2608..2991
                     /gene="mzrA"
                     /locus_tag="OQN13_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCS1615459.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            3138..3506
                     /locus_tag="OQN13_002103"
     CDS             3138..3506
                     /locus_tag="OQN13_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCS1615460.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            3544..3849
                     /locus_tag="OQN13_002104"
     CDS             3544..3849
                     /locus_tag="OQN13_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCS1615461.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3852..4256
                     /locus_tag="OQN13_002105"
     CDS             3852..4256
                     /locus_tag="OQN13_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCS1615462.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            4246..4545
                     /locus_tag="OQN13_002106"
     CDS             4246..4545
                     /locus_tag="OQN13_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCS1615463.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4731..5123
                     /locus_tag="OQN13_002107"
     CDS             4731..5123
                     /locus_tag="OQN13_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS1615464.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            5193..6206
                     /gene="yqjG"
                     /locus_tag="OQN13_002108"
     CDS             5193..6206
                     /gene="yqjG"
                     /locus_tag="OQN13_002108"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCS1615465.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESTEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPQDEMFASVKKRHLT
                     KCL"
     gene            6471..6836
                     /locus_tag="OQN13_002109"
     CDS             6471..6836
                     /locus_tag="OQN13_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS1615466.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            7078..7434
                     /locus_tag="OQN13_002110"
     CDS             7078..7434
                     /locus_tag="OQN13_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS1615467.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(7485..8381)
                     /gene="yhaJ"
                     /locus_tag="OQN13_002111"
                     /pseudo
     CDS             complement(7485..8381)
                     /gene="yhaJ"
                     /locus_tag="OQN13_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            8486..9187
                     /locus_tag="OQN13_002112"
     CDS             8486..9187
                     /locus_tag="OQN13_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCS1615468.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            9210..9374
                     /locus_tag="OQN13_002113"
     CDS             9210..9374
                     /locus_tag="OQN13_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1615469.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(9508..10818)
                     /locus_tag="OQN13_002114"
     CDS             complement(9508..10818)
                     /locus_tag="OQN13_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCS1615470.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVRIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(10846..12177)
                     /locus_tag="OQN13_002115"
     CDS             complement(10846..12177)
                     /locus_tag="OQN13_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCS1615471.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(12452..13816)
                     /gene="tdcG"
                     /locus_tag="OQN13_002116"
     CDS             complement(12452..13816)
                     /gene="tdcG"
                     /locus_tag="OQN13_002116"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS1615472.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(13888..14277)
                     /locus_tag="OQN13_002117"
     CDS             complement(13888..14277)
                     /locus_tag="OQN13_002117"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCS1615473.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQACLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(14291..16585)
                     /gene="tdcE"
                     /locus_tag="OQN13_002118"
     CDS             complement(14291..16585)
                     /gene="tdcE"
                     /locus_tag="OQN13_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCS1615474.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(16619..17827)
                     /gene="tdcD"
                     /locus_tag="OQN13_002119"
     CDS             complement(16619..17827)
                     /gene="tdcD"
                     /locus_tag="OQN13_002119"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCS1615475.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(17853..19184)
                     /gene="tdcC"
                     /locus_tag="OQN13_002120"
     CDS             complement(17853..19184)
                     /gene="tdcC"
                     /locus_tag="OQN13_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCS1615476.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(19206..20195)
                     /gene="tdcB"
                     /locus_tag="OQN13_002121"
     CDS             complement(19206..20195)
                     /gene="tdcB"
                     /locus_tag="OQN13_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCS1615477.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(20294..21232)
                     /gene="tdcA"
                     /locus_tag="OQN13_002122"
     CDS             complement(20294..21232)
                     /gene="tdcA"
                     /locus_tag="OQN13_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCS1615478.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            21421..21765
                     /gene="tdcR"
                     /locus_tag="OQN13_002123"
     CDS             21421..21765
                     /gene="tdcR"
                     /locus_tag="OQN13_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCS1615479.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
BASE COUNT         5797 a         5649 c         5166 g         5510 t
ORIGIN      
        1 gcgttgattc cctgcattaa ctaatttttg ggcagcattt acttctaact tagcaatagg
       61 ttgctgaggt ggaacactaa caacaatccc ccaaattaag ttaactgtta actgcataga
      121 gagatctttt ttatctgtgg caacgtcatc aatactggga accgcttcaa atttaacgcg
      181 ataatttttt tctttttgct ctggttccat tgcaacaaag cgcacagttt tgctactacc
      241 ggctggtaaa gcgaatttag gtggcataac aaccagatga tttgcatcac cggatttaat
      301 ctcaacttca ttttcctgag gcgtcgatgg attatcgata cggaatactg tcgccttgat
      361 gtactgaact tcattacttt tagatattac gcgaacgttg ccttcaccct gactattaat
      421 ggaaacagcc ataggataaa cagtcatatt tgactgtgcc aaaaaaggag cgaacacgaa
      481 taaaagcggc gtaagccgtg ataagagatt aattttcgcc accatataaa tttgatctta
      541 caatattgtt tccaaaaagt tggtgtatta gagatgtaga tttattgtaa gtcaatataa
      601 aaaagcattt ttagttaata ttaaatttaa aggctgggaa ttaacttact gaaatttagt
      661 agctttcaca atgacgatac ttatttttta ggaatttata aatattcaca atattaattc
      721 aatacattaa ttcaatcacc aaatttatat atgaccaatg gcatacccat gaataaagtg
      781 gtatgataac ttcgtccgca ttccactccg ggagacgcat atggaaatca ctgaaccacg
      841 ccgtttgtat caacaacttg ccgctgacct gaaagagcgc attgaacagg gcgtctatct
      901 ggtgggtgat aaactgcctg ccgaacgctt tattgccgat gaaaagaacg tcagccgaac
      961 ggtagttcgt gaagccatca tcatgctgga agttgaaggc tatgtggagg tgcgtaaagg
     1021 ttcaggtatt catgtggttt ccaaccagcc gcgccatcag caggcggctg acaataatat
     1081 ggaattcgcc aattacggtc cgtttgagtt gcttcaggct cgccagctca tcgaaagtaa
     1141 tattgccgag ttcgcggcaa ctcaggtaac gaaacaagac atcatgaaac tgatggccat
     1201 tcaggaacag gcgcgcggcg aacaatgctt ccgtgattcc gagtgggatt tacagttcca
     1261 cattcaggta gccctggcga cgcagaactc cgccctggcg gccatcgttg aaaaaatgtg
     1321 gacccagcgt agccataacc cgtactggaa aaaactacac gaacacattg attcccgtac
     1381 cgttgataac tggtgtgatg accacgatca aattctcaag gcgttaattc gcaaagatcc
     1441 ccatgctgct aagctggcaa tgtggcagca tctggaaaac accaaaatca tgttatttaa
     1501 cgaaaccagc gacgatttcg agttcaatgc cgaccgctat ctattcgccg aaaacccggt
     1561 agtgcatctc gatacggcca ccagtggcag taaatgaaac tcctgtccga caggcgctgc
     1621 gacgcgcctg ttcgcttcgt cggtaagcga aaagtataaa gtgtcagcct gtgtaaatcc
     1681 tctcgccacc ctcccctgca ttcagcaaaa tcagactcca cggacatgta attttgataa
     1741 cgaacaacgt tgacctttgt tacaattaga ttcaatttga atttatgttt ttgaatgctt
     1801 tcttatctca cgatttaaca gggaatagtt caggctgtgt tgatgtatca aacccgcaga
     1861 acataccaaa acagcaataa cattgcggta gtgcatcttt taaaaccagc gtggcgttaa
     1921 ccgattcacc aggaataatg aatggaactt ttgacccaat tgctgcaagc cctgtgggcg
     1981 caggattttg aaaccctggc caatccatcg atgattggca tgttgtattt tgtcttgttt
     2041 gtaattttgt tccttgaaaa cggcttgctt ccagcggcct ttttaccggg cgacagttta
     2101 ctggtattgg tcggcgtgtt gattgcgaaa ggcgcgatgg gttacccgca aacgattctg
     2161 ctgctgaccg ttgccgccag cctcggctgc tgggtcagct atattcaggg gcgatggctg
     2221 ggcaataccc gcaccgtaca aaactggcta tctcatttac ccgcgcatta tcatcaacgc
     2281 gcacaccatc tttttcataa acacggttta tcggcgctgt taattggtcg ctttattgcg
     2341 tttgtcagaa cactgctgcc gacgattgcc gggttatcag ggctgaataa cgcgcgcttt
     2401 cagtttttca actggatgag cggtctgctg tgggtattga tcctgacaac tctgggttac
     2461 atgctcggca aaacgccgat atttttaaag tacgaggacc agctgatgtc atgcctgatg
     2521 ctgctcccgg tggtgctgct ggtgtttggc ctggcaggtt ctctggtcgt gttatggaaa
     2581 aagaaatatg gaaatcgggg gtaagggatg caaatacctc gcatgtcgct tcgccagcta
     2641 gcctggtccg gcactgtttt acttctggtc ggcacgctgt tgctggcctg gtccgcggtt
     2701 cgccagcaag agtctacgct ggcgattcgt gccgttcatc aaggcacaac gatgccagac
     2761 ggtttttcga tctggcatca ccttgacgct catggcattc ctttcaaaag tatcaccccc
     2821 aaaaacgaca ctctgttaat tacatttgac tccagcgacc agagcgccgc cgcaaaagcg
     2881 gtcctcgaca gaacattgcc ccatggctac atcattgcgc agcaggacaa taacagtcag
     2941 gctatgcagt ggctgacccg gttacgggat aactctcatc gcttcggata acttcctgtt
     3001 atccgaaaca tatcactcac tttggtgatt tcaccgtaac tgtctatgat taatgaggcg
     3061 gtggttaaca ctaccgcttc gttgtctgga caatcgttcc tttgtaatag gtccgaatca
     3121 caatggaagg ttcaagaatg aaataccgca tcgctttagc tgtttctctc tttgctctta
     3181 gtgccggtag ttatgccact accctgtgtc aggaaaagga gcaaaatatc cttaaggaga
     3241 tcagctatgc cgaaaaacac caaaaccaga atcgtattga cggtctgaat aaagccctga
     3301 gtgaagtccg ggccaactgt tcagatagcc agctgcgtgc cgatcatcac aagaaaatcg
     3361 caaagcagaa agatgaggtg gcggaacgcc agcaagattt agccgaggcg aagcaaaaag
     3421 gcgatgccga taagattgcc aaacgcgaac ggaaactggc agaagcgcag gaagagctga
     3481 aaaagctgga agcgcgcgac tactaactca caatagtcac tacttactca cctggagaaa
     3541 actatgtcga aagaacacac tacggaacat ctgcgtgctg agttgaaatc cctttccgat
     3601 acgctggaag aggtgcttag ctcatctggc gagaagtcga aagaagagtt gagtaagatt
     3661 cgtagcaaag cggagcaggc actgaaacag agccgttatc gcctgggtga aaccggtgat
     3721 gccatcgcca aacaaacccg tgtcgcggcg gcgcgtgctg atgagtatgt gcgtgaaaat
     3781 ccgtggaccg gcgtgggaat tggcgctgca atcggtgtag tgctcggcgt tctgctgtcg
     3841 cgtcgttaat tatggcggac actcatcacg cgcaagggcc cggtaaaagc gttctgggca
     3901 tcgggcagcg aattgtttcc atcatggttg aaatggtgga aacacgtctg cggctggcgg
     3961 tggtggagct ggaagaggaa aaagctaatc tctttcaact tttactgatg ctgggcctga
     4021 cgatgctttt cgctgcattt ggtcttatga gcctgatggt gctaattatt tgggcggttg
     4081 atccgcaata tcgcctgaat gcgatgattg ccaccaccgt ggtgttgctg ctactggcac
     4141 tgattggcgg tatctggacg ttacgtaaat cgcgtaaatc caccttgctg cgccatacac
     4201 gccatgagct ggcaaacgat cggcaactgc tcgaggagga gtcccgtgag cagtaaagtc
     4261 gaacgtgaac gacgtaaggc gcaactgctt agccagatcc agcaacaacg gctggatctt
     4321 tccgccagtc gtcgtgaatg gctggaggca acaggcgctt acgatcgtcg ctggaatatg
     4381 ctgctaagtc tgcgctcctg ggcgctggtt ggcagtagcg tgatggcgat ctggacgatt
     4441 cgccatccta atatgctggt ccgctgggcc agacgcggtt ttggcgtatg gagcgcctgg
     4501 cgtctggtta aaacgaccct caagcagcaa cagcttcgcg gttaacttcc ctctggccgg
     4561 agcaattccg gccttatccc tcaaattttt tgaagatttt tgacagtttt ccttgctaac
     4621 aatcatcatt caccacgttt atgattctct ccatcgacag caacgacgct aataccgcgc
     4681 ccttgcacaa aaaaacaatc agcagcctga gtggcgcagt ggagagtatg atgaaaaaat
     4741 tagaagatgt tggtgtactg gtagcgcgca ttttaatgcc gattctgttt attaccgcag
     4801 gctggggaaa aatcaccgct tactctggta cccaacaata tatggaagca atgggtgtcc
     4861 cgggttttat gctgccgctg gtgattctgc ttgagttcgg tggtggtctg gcaatcctgt
     4921 tcggtttcct gactcgcacc acagccctgt ttactgcggg ctttacgctg ctgacggcat
     4981 ttttatttca cagcaacttt gctgaaggcg tcaactcgct gatgttcatg aaaaacctga
     5041 caatttctgg cggattcctg ctgctggcaa ttaccggtcc gggcgcgtat agcatcgacc
     5101 gcctgctgaa taaaaagtgg taagcacgcg ctatacttaa cgataaaaaa cgaggaggaa
     5161 gctcctcgtt tttgctattg gaggagagaa aaatgggtca actgattgac ggcgtctggc
     5221 atgacacctg gtacgatacc aaatctaccg gcggtaaatt tcaacgttca gcttccgcat
     5281 ttcgtaactg gctcactgcc gatggcgcac ctggccccac tggcacaggc ggttttatcg
     5341 cagagaaaga tcgttatcat ctctatattt cactcgcctg cccgtgggcg caccgcacgc
     5401 tgatcatgcg caaactcaaa ggactggaac cgtttatttc cgtttccgta gtgaacccgc
     5461 tgatgctgga aaacggctgg acctttgatg acagttttcc gggagcaacc ggagatacgc
     5521 tctatcaaca tgaatttctg tatcagcttt atctccacgc cgatccacac tacagcggac
     5581 gagttactgt tcccgtactg tgggacaaaa agaaccacac catcgtcagc aacgaatcaa
     5641 cagaaatcat ccgcatgttc aataccgcgt ttgatgcgct gggcgcgaaa gcgggtgatt
     5701 actacccacc agccctgcaa acgaaaattg acgaacttaa cggctggatt tatgacaccg
     5761 ttaacaacgg cgtgtataaa gctggttttg ccaccagcca gcaagcctac gacgaggcgg
     5821 tggcgaaagt gtttgaatcg ctggcgcgac tggagcagat tttaggccag catcgttacc
     5881 tgaccggcaa ccagctaacc gaagccgata ttcgcctgtg gactacgctg gtgcgttttg
     5941 atccagtgta tgtgacccac ttcaagtgtg ataagcaccg catcagcgat tacctgaatc
     6001 tgtatggctt cctgcgcgat atctaccaga tgccggggat cgccgaaact gtcaatttcg
     6061 atcacatccg taatcattac ttccgcagcc ataagaccat caaccctacg gggattattt
     6121 caattggtcc gtggcaggat ctcgatgaac cgcaggacga gatgttcgct tcggttaaaa
     6181 aaaggcacct tacgaagtgc ctctgataat tagctaagtg catgttgttc atatcggatg
     6241 cggcgtgaac gccttatcaa acctacaaaa catttatatt caatatgttg caatgaccgc
     6301 gcaggccaga taagcgtagc gcatctggcg attttgcgct tgtcatcagt cttagcgcgc
     6361 cttcacagca ctttattaat tccttgaacg aatatttact gccatttcat tcacgtctat
     6421 tcttaatttg ctgctttaaa aacaagtgat tgagcaacat tgaggcaaaa atggactggt
     6481 atctgaaagt aataaaaaat tatgtcggtt tccggggtcg tgcgcggcgc aaagagtact
     6541 ggatgtttat tctggtcaac atcatcttta cgttcgtgct ggggctgctg gataaaatgt
     6601 taggctggca acgcgctggt ggcgaaggca tcctgacgac aatctacggt attctggtgt
     6661 ttttaccgtg gtgggcagtt cagttccgcc gcctgcacga caccgaccgc tcggcgtggt
     6721 gggcactgct attcttaatc ccgtttatcg gctggcttat tatcatcgtc tttaactgcc
     6781 aggcgggtac gccaggcgaa aaccgctttg gacctgatcc aaaactggaa ccttaaaaca
     6841 aatctgctcg ccggatgtct gactcatccg gcttttatca tcatgttgat gaatgaataa
     6901 tttactaatg tttatttaaa atatttcaat gtcgttatat aaattaccta taaaaaataa
     6961 ccatgtattt atcttaaata tcaaaacctt tataacatcc gaaaaagaaa gatacgaata
     7021 gcatttacac aatacccgga atagactttc gtcactttta attaaaggga tgtttttatg
     7081 cagtggtatt tatccgtatt aaaaaattat gttggtttct ctggtcgcgc acgtcgtaaa
     7141 gagtactgga tgtttactct gattaacgcc atcgtcggcg ctattatcaa tgtcattcaa
     7201 ttgattttag gtctggagct tccatatctg tctatgcttt atttgctggc aaccttcctt
     7261 cccgtccttg cgcttgctat ccgtcgctta cacgataccg accgttcagg tgcctgggcc
     7321 ttgctctttt ttgttccgtt tattggctgg ctcgtgcttc tggttttttt ctgcacagaa
     7381 ggtacttctg gcagcaatcg ttacggaaac gatccgaagt ttggttcaaa ttaatcttag
     7441 aattggggcg atatttcgcc cctttttatt aacaataata aatattattt tccgctaaaa
     7501 agtttgggaa tttcccgcag acaccaggat ttcgcttccc ccatactgtc acgacgccag
     7561 gccataataa tatcgatctc actggtcgat tccgggctga cgacacgcaa ccgcccttcc
     7621 gcaatatctt tttcgaccat cggatacggc atcgtcgcca cgccaagccc cgccagtaat
     7681 gcctgacgtt tatcttcaat cgtgctcacc gttaagcgcg gctgtttgtc cagcagttgt
     7741 acggtcaaca ccgggcgctc acgagcggta tccgctaccg caattccacg atatttcacg
     7801 cgcgtcactt cagataacgg ttccggctcc tgatgaatcg ggtgatcagg cgcggcaacg
     7861 tagacgttca ttaacgtata gagcttgcgc gagttgatct ccgacgagga acgaaaatgc
     7921 atatccggcg cgataacaat atccgcccgc ccctgctcca gtcgttccta cgcccccgcc
     7981 agcacttcgg tgatgattgc cagttgggta ttggcttttg ccgccagttt gtcgattaac
     8041 gggaaaaagg caggtgtcgg taccagcgct tcggtcacaa tggtgagatg cgtttcccaa
     8101 ccgcgcgcga gggcttccgc atcggtagtc agtttatctg cggcttccag caaaacgcgc
     8161 cctcgctcca gcaacatcct cccgacattg gtgaatttgg tgcgatggcc cgagcggtca
     8221 aacagcacca catccagctc ttcttccagt ttttgcatgg tgtagctaag tgcggaaggc
     8281 acgcgtccca gctcatccgc cgccgccgca aaactgcccc ggcgatcgat cgcatccata
     8341 acccgtagtg cttccagcgt taatgccctt tctttggcca tttcgttctc attcaggaaa
     8401 tttgaacata ccgggcagaa tatctggcta acaatgtagc gtccagcccc ttaccataaa
     8461 aggaagtaaa gagaggtcaa gaattatgat tactacccga actgccaggc agtgtggaca
     8521 agcagactac ggatggttgc aggcccggta tactttttcc tttggacact acttcgaccc
     8581 gaaattgtta ggctatgcct ccctgcgtgt gcttaaccag gaagtgctgg ccccaggtgc
     8641 cgcctttcag ccgcgaacct atcccaaagt cgatatttta aatgtgattc tggatggaga
     8701 agcagagtat cgcgacagcg aaggcaatca tgttcaggcc agcgccggtg aggcgttgct
     8761 gctctctacc cagccgggtg tcagctacag cgaacacaat ctcagcaaag acaaaccgtt
     8821 aacacgaatg cagctttggc tggacgcctg cccgcagcga gagaatccgc tgattcaaaa
     8881 actggcgctt aatatgggca agcagcaatt aatcgcctcg ccagagggta cgatgggaag
     8941 cctgcaatta cgccagcaag tgtggctgca ccatatcgtg ctcgacaaag gcgaaagtgc
     9001 gaatttccag ttgcatgggc cacgcgcgta tttgcaatcg attcacggga aatttcatgc
     9061 gcttacgcat catgaagaga aagcggcgct gacctgcggt gatggggcgt ttattcgtga
     9121 cgaggctaac attacgctgg ttgccgattc cccactgcgc gctttgctga tagatttgcc
     9181 tgtctagttg tttttacagg gagatgatga tgagtaaaaa atcggccaaa aagcgccagc
     9241 cagtgaagcc cgtggtggcg aaagaacctg ctcgcaccgc caaaaatttt ggctatgaag
     9301 agatgttgag cgagctggaa gccatcgtcg cggatgctga aacgcgttta gccgaggatg
     9361 aagctaccgc gtaaagacag gttgccggat gcggcgaaaa cgccttatcc ggcctacaaa
     9421 atccaacaac attaacgaat taaacaaccc gtaggccgga taaggtgcgc cagcatcgca
     9481 tccggcacaa tactcaaaac ctggcgttta tctggccttg ctcgccataa tctcgataat
     9541 ctgccgatcc gtttgctgca tcgaatggct tgctaacgca cacaggttgg caatcgactg
     9601 ctcaacatca tgcgccacga tcccttcatt gccggtcacg gcggtatcat ccagcgccat
     9661 taacaccgct ttccacgcag ccgaagcact ggtcgaaacc ttcatcgcgc agctgttcga
     9721 cgcaccatcg caaatcatgc cgctgacatc gccgatcata ctgctgatcg ccatcgagat
     9781 agtttcataa cgcccatcca ccagccatgc catcccggcg gcggccccca ttgctgcggt
     9841 cgttgcggca caaagtgcag acaaacgcgg taactggtta tggatgtaaa ttgcgctcaa
     9901 atgagaaagc atcagcgcac gcgccagtcg ttcatcatcc gctccgaagt gttctgctac
     9961 aaccaccaca ggcattgttg cggtgatccc ctggttaccc gagccggagt tactcatagc
    10021 cggaagcgta gcgccgccca tacgcgcatc ggatgccgcg ctggtacgaa tcacaatgga
    10081 tgaagagaga tctttcgcca gcaaaccgcg ctcgcactgt ttttccagcg tcgcgccaat
    10141 atgcagcccc cacttaccgc tcaaaccttc ctgcgataac gcacaattta gcttcgcgga
    10201 atcgagaata aagcggatcg ccgcaaacgg gacttcattg acgaacttca ggatctcagc
    10261 cagcgtcgtt ctggaaagca ccgtcagcgg agactcttgc tcgccctctg ccacacacgc
    10321 ctgctgggta aacaccacac cattgtgcgt ctcaatacgc acaatgttgg tatgcccgcc
    10381 gacgatggtg acacacgccc acttctcacc gttccagact ttggcgcgtg agaagaggat
    10441 ttcattgcaa ggttcctgga tcttaacgga gactttcccc gccgccagca gtgctttggc
    10501 atcggcaatt gcctgcgcag ttgcgtcttt cagcacttcc agcccggcgt tggcatttcc
    10561 acctaacgcc cccagcgccg ccgcaatcgg cagccccacc attcccgtgc cgggaacggt
    10621 gacgcccaga ccgttcttca tcagatttgg cgaaacccag gcttctacac gttcaaccgg
    10681 accttccagt tctgccgcag caaccgccgc cgccagcgcc agtgaaatcg gttcagtaca
    10741 tcccagcgcc ggttttactt cctcctgaac ggcgaggatg taacgctgcc ataacggatt
    10801 taaagtcgaa tcaaacatat taaaaacctt aaaatttcag gtaaatcaag aaaatgccag
    10861 gaacggagaa acacaaagca acaaaccagt gacgataatc aggtacagag acatcccttt
    10921 gtatttgtgc aatgccggta ctttgtaaac cagccacgcc gggatcaggc accctaccat
    10981 gccgaaaatc gggctacaga tagaggtgaa gctcaacacc ggtgcgttca gtacgatagc
    11041 gctccaggcc agcaaaatgg cgaaaatcat gatgccgcgc tgaacaagat tttcgttaat
    11101 cttctcggca ggcatcttgc gacgcaggat gttcattacg atcccttgcg ttgcttcgcg
    11161 aaagcctaaa tagacgccaa agaatgcagt catgacagca aagatattga gaatgacgct
    11221 gaccactttc acccacgctg cgccgtcacc gctaataaac tgtgcggcaa tcgccagcgc
    11281 ggaaatattc tgctcgtagg cttttaccgc ttcgtcatgc cccatcgcca gcgtgaacga
    11341 cacggcgtag aaaaagacgg tgacaaacaa aatgccaaac gcgatattca ttgcccgcaa
    11401 tgctttatgc cgcgccactt caatagattt ttcccgcgag cgataagaga tcaccatcgg
    11461 gcttaacgtc tggataaaca aaatcgacgt cagggtaaac ggcagcgtaa taatggcgtt
    11521 tttcaccagc agccccagcg gcggtagcga accgacgtta tacagatgcc acattcccac
    11581 catcgacacg cccagcgccg cgaccaccag cagcttggtc agcaccatgc cggtcgaaat
    11641 tttgaataac aatttctcgc cgcgtgaaga gatcgccacc agaatgcaaa tcagtaccag
    11701 accataaaag ggactgtctg acagcaaccc ttccgtcacg ccgaaggtat gcagatagga
    11761 agcactatcg ttggtgatgg cggtggaata gacgaacatc caaatcacca gcattacgaa
    11821 atagagcgca cctaacagga tgccccagtt tttacctaaa taaccgctaa tgacactcgg
    11881 gtaatcttta cattctggtg attctgccag cgtattaata aacaaccgct gaaacagata
    11941 cattgccggg taaccaatca ccgatgagag caaaaatacc cacaatccca tcaaaccgac
    12001 ctgcaccggg agaaaaacaa tccccgcgcc aatcgccatc ccgatactca taatcaccca
    12061 gccggtgtca gtactgtcga atttgatcgc ttctcgccac tcgctctcac tcattccagc
    12121 acgacccgcc ggggtcgaag cgtctgcaat gacgccttta ttcgatgcga tttccataat
    12181 ttctcgctca atattttgta gggcttatta tttttttccg agccgcatca aggcgatatg
    12241 cggtctacgt ttttgcaggc gagttattag aataaagaaa tgatacgcgc gaggtcagag
    12301 aaaatcttca caatcaagac tttgaaataa cggagatgga taagaatttt ctactaaatt
    12361 aatcgcaaga gaatattcat tctctatttg cggcagatca caaaaaaaag gtgcacattt
    12421 gtgcacccaa ggatgaaagc tgacagcaat gtcagccgca gaccacttta atggccagtc
    12481 ctccgcgtga tgtttcgcgg tatttatcgt tcatatcttt gccggtttca tacatcgtct
    12541 cgatcacttt atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc atccacgcgg
    12601 cgtttactgc tttcacggca ttaatggcat tacgttcaat gcacgggatt tgtacctgtc
    12661 cggcaaccgg atcgcaggtc agcccaaggt tatgctccat cgcgatttcc gccgcattgc
    12721 atacctgcgc cggactgccg cccagtagtt cagttaaccc tgccgccgcc attgaacagg
    12781 ccacgccaat ctccccctga cagccgacct ccgcgccaga gatggaggcg ttcattttat
    12841 acagcgcgcc aatagccccc gctgccagaa aatagcgggc aattgaccgc tcgtttaccg
    12901 gacgacggaa cttatcgtaa taagccagca ctgccggaat aatgccgcac gcaccgttag
    12961 tcggtgccgt taccacgcgc ccgccagctg cgttttcttc actaaccgcc agcgcgtaca
    13021 tgttgatcca gtcgatgaca ttcatcggat cgttagagat gttatcgcta gaaaccagct
    13081 gacgacgcag cgctacggca cgacgcggca cattgagcgg accaggcagc acgccttcag
    13141 tgttcatccc gcgttcaata ccgtcatgca tcacttgcca gatacgggca aatcctgcgt
    13201 caatttccgc tttgctgcgc agcgccagct cgttgtgcat catcaggcca gatatagaca
    13261 ggccgttgta atcacacatt ttcagcagtt cacctgctga gtggaaatcg taaggtacgg
    13321 acgtttcgac atcgtgcgac aggccgaagt gttcttcttc gacaataaac ccgccgccga
    13381 cagagtaata ggttttactt aatagcgctt cctgtccctt ccaggcagtg atccgcattc
    13441 cgttctcatg gcgaggcaac atttcgggat ggaagataat gttctttgct acaggaaaat
    13501 caacaatatg cgcaccagat gccactggca gccgcccgct gcgcgttact aactctataa
    13561 atgcagggat ctcatcaatg acaacatcct gcggactgtt tcctgccagt cccatgataa
    13621 tggcgacatc cgtggcatgg cctttgcccg tcagtgacaa cgacccatac agatcgacca
    13681 caatatggct cgtcgcggtt aataagccgc tactttccag ccgatcaata aaactttttc
    13741 cggcattcat tggccccacg gtatgcgaac tggagggacc aatcccaatt ttgaaaatat
    13801 cgaatgcact aatcatatcc acaccctcgg attgccgttc agtgaagtgg agcggaacga
    13861 ccttgcgacc gtcccgctca cgaggcttta cgcactacgt actgcgatag cttcaatttc
    13921 cagcttcaca tctttcggca aacgcgcaac ctgcacacag ctccgggtcg ggtaggtcgc
    13981 ctgatgctca tcgaagaact gcttatagac ttcattgatg gtggcaaaat cattcagatc
    14041 ggtgatgaac acggtcatct tgatgatatc gcccacgctc agcccggcag caaccacgat
    14101 cgctttgacg ttttcgaggc ttaaacacgc ctgatcttgc acatcagccg ggatctcacc
    14161 ggtctgcggg caaaccggaa tttgcccgga ggtgaagacc atgctgccta aatcaacgcc
    14221 ctgaacataa gggccgattg cgcctggggc acgttgcgtt tcgataatct ttttcataca
    14281 tcctccggcg tcagagcgcc tgggtaaaag tacgtgaaat aacatcctgt tgctgttcac
    14341 gggtcagtgc gttgaagcgc acggcgtagc cagagacacg gattgtcagg ttaggatatt
    14401 tttccgggtg ctcgatggca tccagcagca tttcccgatt cattacgttg acgttgaggt
    14461 gttgaccgcc ttcgacatcc gcttcgtggt ggaaataccc atccagcagg ccgacaaggt
    14521 tggttttacg tactggatct tctttgccca gcgccgcagg aacgattgag aaggtgtacg
    14581 agatcccatc tttggcgtag gtgaacggca gtttcgccac cgacgtcaat gaggccacgg
    14641 cacctttgcg gtcacgacca tgcatcgggt tagcgcccgg cgcgaacggt gttccggcgc
    14701 gacgaccgtc cggcgtatta ccggttttct ggccgtacac cacgttagaa gtgatagtca
    14761 gaatcgactg ggtagggacg gcgttgcgat aggttggcag cgctttaatt ttcttcataa
    14821 agcgttcaac caggtcgcag gcaatgctgt ctacgcgctc gtcgttgttg ccgtactgcg
    14881 gatattcacc gtcgatttca aagtccaccg ccaggccgtt ttcgtcacgg attggtttca
    14941 cgcgggcata tttgatggca gacagggagt ccgtcgccac cgacaggccc gcgatgccgc
    15001 atgccatagt gcgatagaca tcacgatcgt gcagcgccat cagcgaagct tcgtagctgt
    15061 acttgtcgtg catgtagtga atgatattca gcgcgctgat gtactgcacc gccagccagt
    15121 ccatgaagtg atcgaggctg tccatcactt tgtcgtaatc cagcacgtcg tccatcagcg
    15181 gtgctgtttt cggcccgacc tgaatcttca gcttctcgtc caccccgccg ttaattgcgt
    15241 acagcagtgt tttcgccagg ttagcgcgtg caccaaagaa ctgcatttgc ttaccaatca
    15301 ccattgggct gacgcaacaa gcaattgcgt aatcgtcgct gttgaagtca gtacgcatca
    15361 gatcgtcgtt ttcatactgc aaggaagagg tgacgatcga cacctgcgcg gcatattttt
    15421 tgaaggcaat cggtaattct tccgaccaaa gaatggtcag gttaggttcc ggtgccggcc
    15481 ccatagtgtg cagggtgtgc aaatagcgga aggagttttt ggtcaccagc gtacgaccgt
    15541 ccagccccat cccgccgatc acttccgtcg cccagattgg gtcgccggag aacagcgaat
    15601 caaattccgg cgtacgcaag aagcgcacca tacggatctt cataatgaag tgatcgatca
    15661 gttcctgtgc ctgctgctca ttgagtacgc ctgctttaaa gtcgcgctca atgtagatat
    15721 cgaggaacga cgccgtgcgc cccagcgaca tcgcgccgcc gttctgtgat ttcactgccg
    15781 ccagataagc gaagtagagc cactgcaccg cttcctgcgc attctgcgcc gggcgagaga
    15841 tatcaaagcc atatttcgcc gccatttcct gaatctgcaa cagcgcatga cgatgctctg
    15901 ccagctcctc acgcagacgg atggtggctt ccagatcttc gcctttttcc agacgggact
    15961 ggagatcggc aaattgcagt tcgcgttcac gcaccagata actgatgcca tacagcgcta
    16021 cgcggcgata gtcaccgata atgcgcccac ggccatagcc atctggtaaa ccggtcagca
    16081 cgccagattt acggcagcgc agcatatccg gtgagtaaac atcaaatacg ccctggttat
    16141 gggttttacg cagatcggta aacagatatt caaattcact gtccatttca cggccatagg
    16201 cgtggaatga acttttaatc atattaatgc caccgaacgg atgcagcgcg cgctttaatg
    16261 gcgcatccgt ttgcagacca acaatttttt ccagcggctg gttaatatat cccgcatcat
    16321 gagcggtaat tgtggtggca atattggtat cgaaatcaac cggcgcgtgg gttgcgtttt
    16381 cgatacggat gccttccatt actttttccc acaattccgt ggtggcaggc gtcgcttcgg
    16441 cgaggaaaga ttcatcgcct tcatacggtg tatagttatg ttgaataaaa tcgcggacat
    16501 taatttcgtt tttccagtcc gtacctttaa agccaagcca tgcgtcggcg tacagcttat
    16561 cgctggtatc aatatctatc ttcatgaaaa ataatctctc tacaatactt caactaaatt
    16621 atgcaaattc tgcgggcgcg ttaactttgc ctaaatgaat ggcatccaaa gcaatcattt
    16681 tttcttcgtt agtcggaata acggcacaaa tgacacgcgc attttcactg gaaacaattc
    16741 gctctccaaa agagttagag cgattattca tttctgtatc aatcactacg cctaatacag
    16801 ccaaatgttc catgaccaga cgacgaatta aacttgaatt ctctcctatt ccgccggtga
    16861 atataattcc atccaggcga cgtaatgaag ctgcgtgtcc ggcaatatga cgggcaattc
    16921 ggtgaacaaa ggttttaatt gccagttgcg cgcgttcgtg accttcatgc caggcttttt
    16981 ccagaacacg taaatccgaa gaaagaccgg aaatacctaa taatcccgac tctttattca
    17041 ctacgcgttc caggtcaccc aggctctggt tggtttggct ggcgacccag gacatcgcac
    17101 caaagtcgac atcgccactg cgggttccca tcatcaagcc ttccagcggc gtcattccca
    17161 ttgaagtatc aacactctga ccgttgcgaa ccgcgcagat tgacgcgcca ttgccaagat
    17221 gcgccacaac caggccggag tcatcttccg ccagattcag cagagaatgt gcgcgctggg
    17281 aaacatagcg gtgcgacgtg ccgtggaaac cataacggcg tacacctaac tcttcataat
    17341 atttccacgg caggccgtat aaataagctt ccggagccat cgtctggtgg aaactggtat
    17401 caaataccgc cacctgagtt acgcccggaa ataattgctg cgccgattca ataccactta
    17461 aattggcgta attatgcagg ggtgccagtg gagaaacgcg acggatatta tcaatgactt
    17521 catcggtaat aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg cggtggccaa
    17581 ttaaggccac actgtcattt aaattccgtt tttccagttc aaatgcaatt gccttcaatg
    17641 caccttcgta gctgtggtga gccagcggtg ctggctctcc cccatttacg gataagaatg
    17701 cattttccga gttaataccg tcggcaatac ctgacattaa tacttcacag tcgctggcat
    17761 cgagcacgga aaacttaatc gaagacgaac cacagttaat aaccaaaaca accggaaatt
    17821 cattcatctc ttttctcatc ctgagttacg gattaaaaca gtttgtatac gatgttcagg
    17881 atggtcagca gaccaatcac ggtaacaaac acgttatcca gacgaccacg gtatttcgcc
    17941 agagacggcg ctttacggat ggcatacatc ggcaacaggc acagcaggga tgcgataatc
    18001 ggtgcgccca tggcttcaat caggtcgagg atgttcgggt tggcgtaggc aacaacccag
    18061 gtggagccca tgatgaagat catgctgata gtgttcagtt tacccagcga cactttagtt
    18121 ttgtcgcctt tataaccaaa cttcaggacc aggccattca gaccttccag cgttcccaga
    18181 tagtgaccga agaaagattt gaagatagcc acgagtgcga tgatggaagc cgcatattcc
    18241 agtgtaatcg cgaacgttgt tttggtaccg gtcatggacg caaagtggtt agccagataa
    18301 gaaagcactg gaatattctg cgctttggct tccgccatgt tggccggaga cagcgtaaat
    18361 aggcagctaa aggcaaagaa catcaccact gcaaccatca gcatgctggc acgagaaatg
    18421 atttgggaac atttacgttc ggtgaagtcg cgaccgaagt ctttctcata ctcttcacgt
    18481 ttagaaacca cgaaggaaga gacgattggc gagaaattaa aggagaaaac catgatggaa
    18541 atccccagcc agacggtgat caggataccg tcatgaccgg ttaacgacag cgaaccgagg
    18601 tcaacctggt cgataactgc agagttccag taagggatca gcgacagaga aatcagcacc
    18661 aggctggcga taaacggcca taccaggtag ctcatcactt taaccatcag atccttacca
    18721 aaccagatga cgaaagccat cagcagcagc aggaacagcg ccacaaagcc gcgattcagc
    18781 ggtgcaaagc cgagctggtt ttcccagaac gtcataaagg tattggtaat agtaacgcca
    18841 taaatccaca gcagtgggca aatcgcgaag aagtacagga acgtgataac cacgccgcca
    18901 gttttaccaa aatgctcttc caccgtttcc gtaatgttgc cggaagggtt agagccagaa
    18961 agacatagac gcgccagcgc ccggtggcaa taaaatgcga tggggtatgc caataccaac
    19021 atcagaagaa tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa cagcaccccg
    19081 gcgccgattg ccgtaccaaa caagcctaac gtccatgtgg tatctgattt acgccaggac
    19141 gattgttttg tctggctgga tacaatgcta tctgaagtac tcatatccta tcctcaacga
    19201 attaattaag cgtcaacgaa accggtgatt tgagagacgc gagaaagatc gatattgccg
    19261 ccggaaataa tactgacggt ttttctgttt tgaatatatt ggtctaattt accgcttaat
    19321 aatgcagcac atgccagagc gcctgcgcct tcggtgacga ctttattgcg ctgaattaag
    19381 gcaatcatac tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc gactaattca
    19441 cgaacgattt cgtaagttaa attacccggg cgggagacat cacaaccatc cgccagggtg
    19501 ccggtagttc ggtgcgtggt tatttctccg gagtggaaag aagccgccat gccgtgaacg
    19561 ttttcagact gtacgccaat aacacgaatg gtcgggttaa tagatttaat tgccaccgca
    19621 ataccagcaa ttaaaccgcc accaccaatt ggcacaatca cgttatcgac atcatagaga
    19681 tcttccataa tttccagacc aatcgttccc tggccagcaa tcactttcgg atcatcgtaa
    19741 ggtgggataa aaatacggcc ttccatttcg acaatttcgc tcactttagc gatagtgtcg
    19801 ttgaagttat caccatgcag aacgacttct gcggagtagt cgcacgttgc cgctactttg
    19861 gattttggcg caccttttgg catcaccact ttaccgtcga tacccagcat cgcgcaggag
    19921 agcgaaaccc cttgcgcatg gttgcccgca gaacaggcca ccacgccttt gcgtttttcc
    19981 gcatcggtca gtgaacttaa tttattaaat gcgccacgaa ttttaaatga acccgtacgc
    20041 tgcatatttt caaacttcag gaatatttca cctttgcaac gttcactaaa atagttggag
    20101 cgaggcatgc ctgttttata aattcgccca gccagtcgtt gtttcgcttc aataatatca
    20161 tcaatagcaa tcggcagatc gtatgtaata tgcattataa aacctcttcg cctgaattaa
    20221 atatgtaggt aaccgtaacc gacacctgca aaacagacag gtggattatt tatgtttaaa
    20281 taaaaacaaa taactaacca acttctatta attgccttcg tctacaccca ttataagatg
    20341 aatactcttt ggctaattcc accaaaaccg atgctgcttt tttaatacga taatttttcg
    20401 accataccgc ggcatattgt gccacaggta atgtttcttc aaccggaata gtaataaatt
    20461 gattagaacc aaaaggtgac gtcatatcac aaggaattac agttaagaaa tcagcattga
    20521 gaacaagatt ataaattgtc acgactgagt cggttttaac gatgttttca atactgatgc
    20581 catttctttg taacgtagta agcagttcgc tgtagtaccc catattagtt tgtggcaaca
    20641 cccactgttc gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt cgggacttac
    20701 tggctaccag cacaaactcg gactcgaaca gcggctcaac atgtaaatcc tgaagcttca
    20761 tttctgcact taatgtacca atcgcaaaat ccagtcgacc gtcgcggatt gccggtaaga
    20821 acgaagacag ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc tctttgaact
    20881 tgttgatcat ccctgacata aaagtaaaac caatcaatga aggaaaacca aatgagactt
    20941 ccaccaccgc ctcagaagac ataccgctta tctcattaac catatttttc atttcacggg
    21001 taatggattc ggaacgggag agtaacaatt gaccggcagg tgttaatgtt acaccggtat
    21061 ttttccgcac cactaattcc acaccaaaat aatcttcaat atcgttaatg attttactga
    21121 cggccggttg agttaaccct aattcttttg cagccgagcc gatagagcca cttctaatga
    21181 cttcctgaaa gactaccagg tgctgcgttt tcggaagaag aatagtgctc ataacgacct
    21241 acgttaatta cctcattgac ggcatgaagt gtatcaaaat gaaatgaaca ggatatgtgc
    21301 gaccactcac aaattaactt tcaatacttt ccagggtatc gtcattacaa ttaaatacct
    21361 ttaaatatca acaagttaaa gcataaaaat cagcataaac cctgattttt gtcaaaaaaa
    21421 atgacgggga taaccatatt ttatggcgat aacatcattc gttatgtggt taatacaaaa
    21481 aaggggctga gaccatattt caagcaatta ccggataatt accaggcaaa atttgagtta
    21541 aatttaatga gcaagttctc aaattttatt ataaataaac cattttcagc gataaatact
    21601 gcggcacgtc acattttttc acgctatttg ttggagaaca aacatttatt ttatcaatat
    21661 tttaaaattt cgaatacagg tattgatcat ctcgaacaat tgattaacgt caactttttc
    21721 tcttctgaca ggacgtcatt ttgtgaatgc aatcgttttc cataaattct tctcccctca
    21781 taggcgacga atagcatttt gtgttgagga tcacaaaacg aataattgct gatcgccgcg
    21841 ataaggtcag acaaagacaa caagggaaat tttcacagag cttttgatcg tcgtaggcca
    21901 cagaatgttg cttcgtttac tgtaacgccg ggtaaatgag cgttttttga tagtgcgaaa
    21961 gaaccctgcg aggaaaataa gcatctatta ttgtttgtca tgacgacaag gcaggcatca
    22021 atgaaaggct tcccaattgc acatattttt cacccttcaa tcccgccaat gcacgcagtg
    22081 gttaacaatc acaatagaaa tattgattat tggacggtaa aa
//
LOCUS       DAJVRF010000061        21645 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJVRF010000061 DAJVRF010000000
VERSION     DAJVRF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21645
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.061"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..978)
                     /gene="cmtA"
                     /locus_tag="OQN13_002124"
     CDS             complement(<1..978)
                     /gene="cmtA"
                     /locus_tag="OQN13_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCS1615480.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLV"
     gene            complement(1006..1449)
                     /gene="cmtB"
                     /locus_tag="OQN13_002125"
     CDS             complement(1006..1449)
                     /gene="cmtB"
                     /locus_tag="OQN13_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCS1615481.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            complement(1763..3754)
                     /gene="tkt"
                     /locus_tag="OQN13_002126"
     CDS             complement(1763..3754)
                     /gene="tkt"
                     /locus_tag="OQN13_002126"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS1615482.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            4032..4790
                     /gene="loiP"
                     /locus_tag="OQN13_002127"
                     /gene_synonym="yggG"
     CDS             4032..4790
                     /gene="loiP"
                     /locus_tag="OQN13_002127"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCS1615483.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4933..5853)
                     /gene="speB"
                     /locus_tag="OQN13_002128"
     CDS             complement(4933..5853)
                     /gene="speB"
                     /locus_tag="OQN13_002128"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCS1615484.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5991..7967)
                     /gene="speA"
                     /locus_tag="OQN13_002129"
     CDS             complement(5991..7967)
                     /gene="speA"
                     /locus_tag="OQN13_002129"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCS1615485.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7976..8107)
                     /gene="yqgB"
                     /locus_tag="OQN13_002130"
     CDS             complement(7976..8107)
                     /gene="yqgB"
                     /locus_tag="OQN13_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCS1615486.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            8243..8458
                     /locus_tag="OQN13_002131"
     CDS             8243..8458
                     /locus_tag="OQN13_002131"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1615487.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            8762..9916
                     /gene="metK"
                     /locus_tag="OQN13_002132"
     CDS             8762..9916
                     /gene="metK"
                     /locus_tag="OQN13_002132"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCS1615488.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            10352..11746
                     /gene="galP"
                     /locus_tag="OQN13_002133"
     CDS             10352..11746
                     /gene="galP"
                     /locus_tag="OQN13_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCS1615489.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11823..12320
                     /locus_tag="OQN13_002134"
     CDS             11823..12320
                     /locus_tag="OQN13_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCS1615490.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            12415..13122
                     /gene="endA"
                     /locus_tag="OQN13_002135"
     CDS             12415..13122
                     /gene="endA"
                     /locus_tag="OQN13_002135"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCS1615491.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMYNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            13202..13933
                     /gene="rsmE"
                     /locus_tag="OQN13_002136"
     CDS             13202..13933
                     /gene="rsmE"
                     /locus_tag="OQN13_002136"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCS1615492.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13946..14896
                     /gene="gshB"
                     /locus_tag="OQN13_002137"
     CDS             13946..14896
                     /gene="gshB"
                     /locus_tag="OQN13_002137"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCS1615493.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTENGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            15005..15568
                     /locus_tag="OQN13_002138"
     CDS             15005..15568
                     /locus_tag="OQN13_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCS1615494.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            15568..15984
                     /gene="ruvX"
                     /locus_tag="OQN13_002139"
     CDS             15568..15984
                     /gene="ruvX"
                     /locus_tag="OQN13_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCS1615495.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(16159..17139)
                     /locus_tag="OQN13_002140"
     CDS             complement(16159..17139)
                     /locus_tag="OQN13_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCS1615496.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            17157..17861
                     /gene="yggS"
                     /locus_tag="OQN13_002141"
     CDS             17157..17861
                     /gene="yggS"
                     /locus_tag="OQN13_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCS1615497.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17879..18445
                     /gene="yggT"
                     /locus_tag="OQN13_002142"
     CDS             17879..18445
                     /gene="yggT"
                     /locus_tag="OQN13_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCS1615498.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            18442..18732
                     /locus_tag="OQN13_002143"
     CDS             18442..18732
                     /locus_tag="OQN13_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCS1615499.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIVALIN"
     gene            18740..19333
                     /locus_tag="OQN13_002144"
     CDS             18740..19333
                     /locus_tag="OQN13_002144"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCS1615500.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            19326..20462
                     /gene="hemW"
                     /locus_tag="OQN13_002145"
     CDS             19326..20462
                     /gene="hemW"
                     /locus_tag="OQN13_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCS1615501.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            20773..>21645
                     /locus_tag="OQN13_002146"
     CDS             20773..>21645
                     /locus_tag="OQN13_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCS1615502.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSEGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQKQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEI"
BASE COUNT         5354 a         5635 c         5514 g         5142 t
ORIGIN      
        1 caccagggta gctaccgtaa caccggcaat tgtcgccagg aacgagcctt tcggcgtcag
       61 tgccaggtaa gcaaagatag accccggact tgggccagcc accagaccac cgtccagtaa
      121 gttaaacacc caggtgccag acataccgcc cgcaatcatg gcaataatgg tcagcggctt
      181 catcagcaca tacgggaaat acagttcgtg gatcccaccg aggaagtgaa taatcatcgc
      241 cccgggcgca gaacgtttac tcatcccttt accaaacaag gtaaacgcca gcagcagccc
      301 taagccaggc ccaggattgg acgccaccat aaagaagatg gatttaccgt taaccgaagc
      361 ctgttgcatt cccagcggat aatagacgcc ctgatcgatc gcattattga ggaaaagcac
      421 tttcgccggt tcattgatga ctgacagcag aggcagataa cccgcatgta ccagcgcctc
      481 aatacactct ttgacgagag tattggcaat taacaccgcc gggccgataa cttcaaaacc
      541 cagcagacaa aggagcatcc ccgcgatacc taatgagaag ttattgataa ccatctcaaa
      601 accggcaggt atgcgttttt ccagtgcctt atcaacatat tttatgacca gcccaccgag
      661 cggccccata atcattgagc caaggaacat cgggatctct gcgccgacga tcacaccaat
      721 agtacctatt ccgcccatga ccgcgccgcg tttaccgccg accagatgac caccggtaga
      781 accaatcatc acgggcaata aataggtaat catcgggccg acaattttgg cgaaatgttc
      841 gttaggcagc caaccggtgg gaataaataa cgcagtaata aaaccccagg caataaaagc
      901 accaatattg gggatgacca ttgccgtcaa aaagccccca aaagcctgga cctttgcacg
      961 agcagacttg ttttccataa tattatcctg tagaggagag agtaattatc cgcggctgat
     1021 tatgtccgct aattgttttt ctgatgctgc tgttaagagt tgttcgagta tttcttcttc
     1081 gcacagtaat tcacttaacg cctgaatagc gccaatgtgc gaatcggcat cggcagcaga
     1141 aaggccgatg agtaatttta ttggttcgtc attccccgga aaataaacac cttgttcaag
     1201 taatgtcaaa gacatcccgg ttttaagcgc cccacattcc ggtcgcgcat gaggcattgc
     1261 cacgcctggt gcgagaatat aataagggcc attgttaatg gtggaatctt taattgcctg
     1321 aatgtaattc tcgctgatat agtttttatc cagcaatgat accatcgaga aatcgatagc
     1381 ttcctgccaa tcttttgctg aatgtataac tgagattgat gattctggaa aatagtcact
     1441 aagccgcata aattatcctt attttatttt tagggtaccg tccggcggta aaaataaaat
     1501 actcgtgcgg tttaatgaaa gcaattgagc agggaaaccg gtgttttgaa aacagtgaat
     1561 tattaatttc ttatataaca ttgagttata gatataacaa caaatgtcaa tacgcatatc
     1621 gtgatgcata ttacataatt gatatttatt gcatataaat atttgtgatc tacaacacgc
     1681 tttatctatt gcttgtccgc aaacggacat atcaaggtaa taaaaaaggt cgccgaagcg
     1741 acctttttac ccgaaatgct aattacagca gttcttttgc tttcgcaacg acgttgtcga
     1801 cggtgaagcc gaactcttcg aacagcaact ctgccggagc tgactcaccg aaggtggtca
     1861 taccgatgat agcgccattc aggccaacat acttgtacca gtagtcagca atacccgctt
     1921 ctaccgcaac gcgtgcagta accgctttcg gcagtacgga ttcacggtaa gccgcatcct
     1981 gcttgtcgaa tgcatcggta gacggcatgg aaaccacgcg cgccttcacg ccttcggcag
     2041 tcagtttttc gtaggcggca acagccagtt caacttccga accggtcgcg atgaagatca
     2101 gttccggctg accagcacag tctttcagca cataaccacc gcgcgcgatg tttgccagtt
     2161 gctcttcagt acgttcctgc tgcgccagat tctgacggga gaggataagc gcggtcggac
     2221 cgtcctgacg ctcaacaccg tatttccacg cgaccgcgga ttcaacctgg tcacacggac
     2281 gccatgtaga catgttcggg gtcacgcgca gagaagcgac ctgctcaacc ggctggtgag
     2341 tcgggccgtc ttcgcccaga ccgatggagt cgtgggtgta aaccatcacc tgacgctgtt
     2401 tcatcagcgc agccatacgt acagcgttac gtgcgtattc gacaaacatc aggaaggtgg
     2461 aggtgtacgg caggaagcca ccgtgcaggg agataccgtt agcaatcgcg gtcataccga
     2521 actcgcgaac accgtagtgg atgtagttac ccgcagcatc ttcgttgatt gctttagaac
     2581 cagaccacag ggtcaggtta gacggtgcca ggtcagcgga gccgccgagg aattccggca
     2641 acagcgggcc gaacgcttca atagcattct gagacgcttt acggctggcg attttcgccg
     2701 gattagcctg cagtttagcg ataaactctt tcgctttggc gtcgaagtca gacggcattt
     2761 cgcctttcat acggcgggta aattcagccg cttcctgcgg ataagctttc gcgtaagccg
     2821 caaacttctc attccatgca gattctttcg cctggcctgc ttctttcgca tcccactgcg
     2881 catagatttc agacgggatt tcgaacggtg cgtatttcca gcccagctgt tcgcgggtca
     2941 gagcgatttc tgcatcaccc agcggcgcac cgtgggagtc gtgagtaccg gctttgttcg
     3001 gggaaccgaa accgatgatg gttttgcaca tcagcaggga cggtttgtca gtcactgcgc
     3061 gcgcttcttc tactgcgcgt ttgatggatg ctgcgtcatg accgtcgatg tcgcgaataa
     3121 cgtgccagcc gtaagcttcg aaacgcattg cggtgtcgtc ggtgaaccag ccttcaacgt
     3181 gaccatcaat agagataccg ttgtcatcgt agaacgcaat cagtttaccc agcttcagcg
     3241 tacccgccag agagcaaact tcgtgggaga tgccttccat catgcagccg tcgcccatga
     3301 aggcgtaggt gtagtggtcg acaatgtcgt ggcccggacg gttaaactgc gccgccagcg
     3361 ttttttctgc aatcgccata ccgactgcgt tggcaatacc ctgacccagc ggaccggtgg
     3421 tggtttccac accagcggta taacctactt ccgggtggcc cggagtttta gagtgcagct
     3481 gacggaagtt tttcagttct tccatcggca gatcgtaacc ggtgaggtgc agcaggctgt
     3541 agatcagcat ggagccgtgg ccgttggaca gcacgaagcg gtcacggtca gcccaggacg
     3601 gattctgcgg gttgtgtttc aggaaatcac gccacaggac ttcggcaatg tcagccatac
     3661 ccataggggc acccgggtga ccggatttgg ctttctgtac tgcgtccatg ctcagcgcac
     3721 gaatagcatt ggcaagctct ttacgtgagg acattttgac tccagatcgg atgatgaagg
     3781 gcacgccctt aacgacttga cgacagcgcg ttttgggcta cgccggaaaa tttgccaaca
     3841 atttaccgca agccgcgcgt catgtacatg gaacatcctt ttgccgcttc agaaatctct
     3901 ggatcatgct cgcatgttgc gcaatctact cgcccgtccg ctgcgctttt ccttatactg
     3961 agactgagcg tcgattcacc tgcaaacggc gcatttttag aataatcctg accttgtgcg
     4021 gaagagaaaa catgaaaatt cgcgccttat tggtagcaat gagcgtggca acggtactga
     4081 ctggttgcca gaatatggac tccaacggac tgctctcatc aggagcggaa gcttttcagg
     4141 cttacagttt gagtgatgcg caggtgaaaa ccctgagcga tcaggcatgt caggagatgg
     4201 acagcaaggc gacgattgcg ccagccaata gcgaatacgc taaacgtctg acaactattg
     4261 ccaatgcgct aggcaacaat atcaacggtc agccggtgaa ttacaaagtg tatatggcga
     4321 aggatgtgaa cgcctttgca atggctaacg gctgtatccg cgtctatagc gggctgatgg
     4381 atatgatgac agataacgaa gtcgaagcgg tgatcggtca cgagatgggg cacgtggcgt
     4441 taggccatgt gaagaaaggg atgcaggtgg cgcttggtac aaatgccgtg cgagtagctg
     4501 cggcctctgc gggcgggatt gtcggcagtt tatctcaatc acaacttggt gatctgggcg
     4561 agaaattagt caattcgcaa ttctcccagc gccaggaagc agaagccgat gattattctt
     4621 acgatcttct gcgccaacgc ggcatcagcc cggcaggtct tgccaccagc tttgaaaaac
     4681 tggcgaaact ggaagaaggc cgccaaagct caatgtttga cgaccatccg gcatccgccg
     4741 aacgcgccca gcatattcgc gatcgcatga gcgcggatgg gattaagtaa agcctggtgg
     4801 tgtcgaacgt aggtcagata aggcgttcac gccgcatctg acatcaatgg cacatttgtt
     4861 accctgtgcg cattgccgga tgcgatgctg actgcatctt atccggccta cgggtggcat
     4921 cgcatctggt gcttactcgc cctttttcgc cgcttggata tacagcatct ccagcgccag
     4981 cgtcgccgca gccagagcgg tgatttccga ctgatcgtat gccggagcca cttccactac
     5041 gtccatccca acaatgttga gatctttcag gccgcgtacc agtttaatag cgcgatcgga
     5101 ggtcaggccg ccaatcactg gcgtaccggt gcctggtgca aaagcaggat ccaggcagtc
     5161 gatatcaaaa gtcaggtaaa ccggcatatc acccacaatc tgtttcactt gggcgataac
     5221 gtcatccacg ctgcgatcgt tcacctggca ggcgtccagc acggtaaagc cgttgtcttt
     5281 atcaaactcg gtacgaatac caatctgcac ggaatgattc gggtcgatca gaccttcttt
     5341 cggcgcggta tagaacatag tgccgtggtc aaattcacaa ccgttcgcat aggtatcggt
     5401 gtgggcgtca aagtgtacca gcgccatttt gccgaaatgc ttcgcatgag cacgcagcag
     5461 cggcagcgta acaaagtggt caccaccgaa ggtgagcata cgcttaccgg cagccagcag
     5521 cttctcggcg tgcgcctgca gcttttcgct catctcacgg gcatcgccaa aggcatatac
     5581 cagatcgccg cagtccacga cgttcagacg ctcacgcatg tcgaaattcc acgggaagcg
     5641 gttgtgttcc caggccagat tcgtcgaaac ctgacggatc gctgccggac cgtggcgacc
     5701 acccgcacga ccagaagtgg ccatatcgaa cggcacgcca gtaatcaccc agtctgcatc
     5761 gctgtcatac ggctggaagt tcatcggcag gcgtaaaaaa ccaaaggcat tggaaaccag
     5821 tgagttatcg tattgatgac ctaaggtgct catggatgga ctcctattac aaagtcgata
     5881 taaaaaaacc cttccgcgtc gttaggcccg acgaggaagg gttggatttg tcacaataaa
     5941 ttgtggcgga ttatcaccgc taaattaagc ggattcaagt aacacaggac ttactcatct
     6001 tcaagataag tataaccgta caaacctgcc tcgaactctt caaggaactg ttgttgcagt
     6061 tcagcatcaa gatcggtttt cttcacttga tcgcggaact gggttaacag cgttttcgga
     6121 tcgagctgta catattgcag catgtccgcc acggtatcgc cttcgtcaga cagttctact
     6181 tctacgctac cgtcagggaa cacgaacacg tcaaccgctt cggtatcacc gaacaggttg
     6241 tgcatattgc caaggatctc ctgatatgcg ccgaccataa agaaaccgag catcggcgga
     6301 ttctctggat cgtactccgg cattggcatt gtcgtggcaa taccatcacc atcaatatag
     6361 tggtcgatag caccgtcaga gtcacaggta atatccagca gcacagcgcg acgttccggc
     6421 acttgatcca gcccttccag cggcagaacc gggaacaact ggtcgatccc ccatgcgtcc
     6481 ggcattgact ggaacagcga gaaattgacg tacattttgt ccgccatacg ttcctgcagt
     6541 tcgtcgatta tcggacgatg cgcacggttt tgcggatcca gttgcttctg cacttcatgg
     6601 cacatgctca gatagagttg ttctgcccat gcacgttctt gcaggctaaa ggtgccggaa
     6661 gagtagccga tatgaatgtc gtgcagatcc atctggctgt cgtgtaacca ttcacgcaaa
     6721 gaacggcgag ttcccggttc gtgcatttcc tgccaggttt cccacatgct ttgcagcgcg
     6781 cgcggcgcat cttctgcggg cgcggtcggc accgtatatt cgttacgttc cacgccgatg
     6841 atattcgaca ccagcacggt atggtgcgca gtcaccgcac ggcccgattc ggtgattacc
     6901 gtcggatgcg gcagaccatt ttcttcacac gcatcgccaa tcgcccagat gatgttgttg
     6961 gcgtattcat tgaggccata gtttacggag cagtcagact gcgaacgggt gccttcatag
     7021 tccacgccca gaccgccgcc gacgtcgaag cactgaatat tgacgcccag cttgtgcagt
     7081 tccacataga aacgcgcgga ttcacgaacg cccgtggcga tatcgcgaat attcgccatc
     7141 tgcgaaccga ggtggaagtg cagtagttgc aggctgtcga gacgcccggc ttcacgcagg
     7201 gtttcaacca gttgcagtac ctgagtcgca gccaggccga acttcgattt ttccccgccg
     7261 gaggactgcc atttacccga gccctgcgaa gccagacgtg cacgcacgcc cagacgagga
     7321 acgacattca gacgttctgc ttcatccagc acaatggcga tttctgacat cttctcaatg
     7381 accagataga ccttgtgccc catcttctcg ccaattaatg ccaggcggat atattcgcgg
     7441 tctttataac cgttgcagac gatgacgcta cgggtcatgc cagcatgtgc cagtactgcc
     7501 atcaactcgg ctttggaacc ggcttccaga cccagcggtt cgcccgaatg aatcagggac
     7561 tcaatcacgc ggcggtgctg gttaactttg atcggataaa caaggaagta atcgccgtta
     7621 tagccgtagg attccctcgc acgtttgaac gcggcgttaa tggaacgcaa acggtgctgc
     7681 aggatctgtg ggaaacagaa cagtgcaggc agacgctgac cctgtgcttc acgagttttc
     7741 actaactgcg cgagatcgac gcgagcttcc gggacgtccg ggtccgggca cacgctgatg
     7801 tggcccagct cgttaacgtc atagtagtta ttgccccacc aggcaatatt gtaagtacgc
     7861 agcatcttgc tggcttcctg ggagctcatt gcaacctcct gcatggagcg tagtacaccg
     7921 tgttcgcccg ctgacgaagg caaacccata gacatgtcgt cagacatagc gaacctcaaa
     7981 ttattttatt aagtgtaaaa cagttaacga ctatcgcagc ctggaactgc gataacaacc
     8041 cataagcaca tggattttcc agcagtgaat gctgacgctc caactgcgca accggtttct
     8101 ttttcataac attattaagc acataaccga acgtaagtgt gacagttcgg cgaaaccacg
     8161 agaaaactct tgtttttaca agagcgccct tgttcagtcc tcagtaactg taaccagctc
     8221 ttgaatcctg agaagcgccg agatgggtat aacatcggca ggtatgcaaa gcagagatgc
     8281 agagtgcggg gaacgaatct tcaccagaac ggtgagacag gttaagcagc agacaacggt
     8341 tcattatttc gtatcacctc cacggccgcc tgttaagacg aacccacaag ccaaaactct
     8401 gatttcaacc cggctggaag tggcaacacg aaagaaacgt cgtgtgcttt ttatttaagc
     8461 cgcgcgccgc gttttatacc ccacaatggc agaaaattgc aaaagataaa tacgcagaat
     8521 gccggcattg tcaggaaaaa tttccagcca cgtttttaac agaatgagac acgattcaaa
     8581 aaaaagtgga aatcgggtga agaattgacc taaaatagcc atccagatgt taatccatcc
     8641 ataccgatta acactcagac tgccagtgtt tttaacctgc agagtcgtgg taggatccgc
     8701 taccacagaa aatccacaca acagtttgag ctaaccaaat tctctttagg tgatattaaa
     8761 tatggcaaaa caccttttta cgtccgagtc cgtctctgaa gggcatcctg acaaaattgc
     8821 tgaccaaatc tctgatgccg ttttagacgc gatcctcgaa caggatccga aagcacgcgt
     8881 tgcttgcgaa acctacgtaa aaaccggcat ggttttagtt ggcggcgaaa tcaccaccag
     8941 cgcctgggta gacatcgaag agatcacccg taacaccgtt cgcgaaattg gctatgtgca
     9001 ttccgacatg ggctttgacg ctaactcctg tgcggttctg agcgctatcg gcaaacagtc
     9061 tcctgacatc aaccagggcg ttgaccgtgc cgatccgctg gaacagggcg cgggtgacca
     9121 gggtctgatg tttggctacg caactaatga aaccgacgtg ctgatgccag cacctatcac
     9181 ctatgcacac cgtctggtac agcgtcaggc tgaagtgcgt aaaaacggca ctctgccgtg
     9241 gctgcgcccg gacgcgaaaa gccaggtgac tttccagtat gacgacggca aaatcgttgg
     9301 tatcgatgct gtcgtgcttt ccactcagca ctctgaagag atcgaccaga aatcgctgca
     9361 agaagcggta atggaagaga tcatcaagcc gattctgccc gctgaatggc tgacttctgc
     9421 caccaaattc ttcatcaacc cgaccggtcg ttttgttatc ggtggcccga tgggtgactg
     9481 cggtctgact ggtcgtaaaa ttatcgttga tacctacggc ggcatggcac gtcacggtgg
     9541 cggagcattc tctggtaaag atccatcaaa agtggaccgt tccgcagcct acgcagcacg
     9601 ttatgtcgcg aaaaacatcg ttgctgctgg cctggccgat cgttgtgaaa ttcaggtttc
     9661 ctacgcaatc ggcgtggctg aaccgacctc cattatggta gaaactttcg gtactgagaa
     9721 agtgccttct gaacaactga ctctgctggt acgtgagttc ttcgacctgc gcccatacgg
     9781 tctgattcag atgctggatc tgctgcaccc gatctacaaa gaaaccgcag catacggtca
     9841 ctttggtcgt gaacatttcc cgtgggaaaa aaccgacaaa gcgcagctgc tgcgcgatgc
     9901 tgccggtctg aagtaatctt tcttcacctg ctttcaaagg ccagcctcgc gctggccttt
     9961 ttcttttacc tcaccgcggt ggataggcat tcacgccgca tccggcaaaa agctgcccgc
    10021 acaataacat cattcttcct caacacgttt tatcgcaaat tatcatcact actgaaaccg
    10081 attacaccaa taacaccagc caaatatttg taatattttc atattattat tcagttttca
    10141 cagttgttac atttcttttc agtaaagtct taattgcaga taacagcgtt taatctatga
    10201 tgatataact caattatttt catgcactta aaccataact aagataaatg ttagtgtaag
    10261 cgattacact gatgtgattt gcttcacatc tttttacgtc gtactcacct atcttaattc
    10321 acaataaaaa ataaccatat tggagggcat catgcctgac gctaaaaaac aggggcggtc
    10381 aaacaaggca atgacgtttt tcgtctgctt ccttgccgct ctggcgggat tactctttgg
    10441 cctggatatc ggtgtaattg ctggcgcact gccgtttatt gcagatgaat tccagattac
    10501 ttcgcacacg caagaatggg tcgtaagctc catgatgttc ggtgcggcag tcggtgcggt
    10561 gggcagcggc tggctctcct ttaaactcgg gcgcaaaaag agcctgatga tcggcgcaat
    10621 tttgtttgtt gccggttcgc tgttctctgc ggctgcgcca aacgttgaag tactgattct
    10681 ttcccgcgtt ctactggggc tggcggtggg tgtggcctct tataccgcac cgctgtacct
    10741 ctctgaaatt gcgccggaaa aaattcgtgg cagtatgatc tcgatgtatc agttgatgat
    10801 cactatcggg atcctcggtg cttatctttc tgataccgcc ttcagctaca ccggtgcatg
    10861 gcgctggatg ctgggtgtga ttatcatccc ggcaattttg ctgctgattg gtgtcttctt
    10921 cctgccagac agcccacgtt ggtttgccgc caaacgccgt tttgttgatg ccgaacgcgt
    10981 gctgctacgc ctgcgtgaca ccagcgcgga agcgaaacgc gaactggatg aaatccgtga
    11041 aagtttgcag gttaaacaga gtggctgggc gctgtttaaa gagaacagca acttccgccg
    11101 cgcggtgttc cttggcgtac tgttgcaggt aatgcagcaa ttcaccggga tgaacgtcat
    11161 catgtattac gcgccgaaaa tcttcgaact ggcgggttat accaacacca cagagcaaat
    11221 gtgggggaca gtgattgtcg gcctgaccaa cgtacttgcc acctttatcg caatcggcct
    11281 tgttgaccgc tggggacgaa aaccaacgct aacgctgggc ttcctggtga tggctgctgg
    11341 catgggcgta ctcggtacaa tgatgcatat cggtatccac tctccgtcgg cgcagtattt
    11401 cgccatcgcc atgctgctga tgtttattgt cggttttgcc atgagtgccg gtccgctgat
    11461 ttgggtactg tgctccgaaa ttcagccgct gaaaggccgc gattttggca tcacctgctc
    11521 cactgccacc aactggattg ccaacatgat cgttggcgca acgttcctga ccatgctcaa
    11581 cacgctgggt aacgccaaca ccttctgggt gtatgcggct ctgaacgtac tgtttatcct
    11641 gctgacattg tggctggtac cggaaaccaa acacgtttcg ctggaacata ttgaacgtaa
    11701 cctgatgaaa ggtcgtaaac tgcgcgaaat cggcgctcac gattaatctc ctcaggcttc
    11761 ctcccgtcgc ggaggaagcc gcctcttgca gtcatctttt cttcgctcta tcctctgccg
    11821 ctatgaaaac atcccgtctc cctatcgcca tccaacaggc cgttatgcgc ggcctgcggg
    11881 aaaaactcgc ccaggccaac ctgaagctag ggcgtaacta cccggagcca aaactctctt
    11941 acacccagcg cggaacctcc gccggaacag cctggctgga aagctatgaa atccgcctca
    12001 atcccgtttt gctgttggaa aacagtgaag cttttattga agaagtggta ccgcacgaac
    12061 tggcacattt gctggtatgg aaacatttcg gccgcgtagc gccacatggc aaagagtgga
    12121 agtggatgat ggaaagcgtg ctgggtgttc ccgcccgtcg tacgcatcag ttcgaactgc
    12181 aatccgtgcg tcgcaacacc ttcccctacc gctgcaagtg ccaggagcat cagcttaccg
    12241 tacgccgcca taatcgcgta gttcgtggcg aggccgtcta tcgctgtgtt cactgcggtg
    12301 aacagctggt tgcgaaataa ccatctgaac tatcaggaac tttcctgatc tggctgattg
    12361 cataccaaaa caactttcgc tacgttgctg gctcgtttta acacggagta agtgatgtac
    12421 cgttatttgt ctattgctgc ggtggtactg agcgcagcat tttccggccc ggcgttggcc
    12481 gaaggtatca atagtttttc tcaggcgaaa gccgcggcgg taaaagtcca cgctgacgcg
    12541 cccggtacgt tttattgcgg atgtaaaatt aactggcagg gcaaaaaagg cgttgttgat
    12601 ctgcaatcgt gcggctatca ggtgcgcaaa aatgaaaacc gagccagccg cgtagagtgg
    12661 gaacacgtcg ttcccgcctg gcagttcggt caccagcgcc agtgctggca ggacggtgga
    12721 cgtaaaaact gcgctaaaga tccggtctat cgcaagatgg aaagcgatat gtataacctg
    12781 cagccgtcag tcggtgaggt gaatggcgat cgcggcaact ttatgtacag ccagtggaat
    12841 ggcggcgaag gccagtacgg tcaatgcgcc atgaaggtcg atttcaaaga aaaagctgcc
    12901 gaaccaccag cgcgtgcacg cggtgccatt gcgcgcacct acttctatat gcgcgaccaa
    12961 tacaacctga cactctctcg ccagcaaacg cagctgttca acgcatggaa caagatgtat
    13021 ccggttaccg actgggagtg cgagcgcgat gaacgcatcg cgaaggtgca aggcaatcat
    13081 aacccgtatg tgcaacgcgc ttgccaggcg cgaaagagct aacctacact agcgggattc
    13141 tttttgttaa cccctacccc acgcgtacaa ccgcgtgggg agacgacgcg gatttttaac
    13201 tatgcgtatc ccccgcattt atcatcctga accactgacc agccattctc acatcgcgct
    13261 ttgcgaagat gccgccaacc atatcgggcg cgtactgcgc atggggccgg ggcaggcgtt
    13321 gcaattgttt gacggtagca accaggtctt tgacgccgaa attaccagcg ccagcaaaaa
    13381 aagcgtggaa gtgaaggtgc tggaaggcaa gatcgacgat cgcgaatctc cgctgcatat
    13441 tcacctcggt caggtgatgt cgcgtggtga aaaaatggaa tttacgatcc agaaatcgat
    13501 cgaactcggt gtaagcctca ttacgccact tttttctgag cgctgcggcg ttaaactgga
    13561 tagtgaacgt ctgaacaaga agctccagca gtggcagaag attgcaattg ctgcctgtga
    13621 gcagtgtggt cgtaaccggg tgccggaaat ccgtccagcg atggatctgg aagcctggtg
    13681 tgcagagcag gatgaaggac tgaaactgaa tcttcacccg cgcgccagta acagcatcaa
    13741 tacgttgccg ttaccggttg aacgcgtccg cctgctgatt ggcccggaag gcggtttatc
    13801 ggcagatgaa attgccatga ctgcccgcta tcaatttact gatatcctgt tgggacctcg
    13861 cgttttgcgt acagagacaa ctgcgctcac cgccattacc gcgctacaag tacgatttgg
    13921 cgatttgggc taacggagaa gaataatgat caagctcggc atcgtgatgg accccatcgc
    13981 aaacatcaac atcaagaaag attccagttt cgctatgttg ctggaagcac agcgtcgtgg
    14041 ttacgaactt cactatatgg agatgggcga tctgtatctg atcaatggcg aagcccgcgc
    14101 ccatacccgc acgctgaacg tgaagcagaa ctacgaagag tggttttcgt tcgtcggtga
    14161 acaggatctg ccgctggccg atctcgatgt gatcctgatg cgtaaagacc cgccgtttga
    14221 taccgagttt atctacgcga cctatattct ggaacgtgcc gaagagaaag ggacgctgat
    14281 cgttaacaag ccgcagagcc tgcgcgactg taacgagaaa ctgtttaccg cctggttctc
    14341 tgacttaacg ccagaaacgc tggttacgcg caataaagcg cagctaaaag cgttctggga
    14401 gaaacacagc gacatcattc ttaagccgct ggacggtatg ggcggcgcgt cgattttccg
    14461 cgtgaaagaa ggcgatccaa acctcggcgt gattgccgaa accctgactg agaatggcac
    14521 tcgctactgc atggcacaaa attacctgcc agccattaaa gatggcgaca aacgcgtgct
    14581 ggtggtggat ggcgagccgg taccgtactg cctggcgcgt attccgcagg ggggcgaaac
    14641 ccgtggcaat ctggctgccg gtggtcgcgg tgaacctcgt ccgctgacgg aaagtgactg
    14701 gaaaatcgcc cgtcagatcg ggccgacgct gaaagaaaaa gggctgattt ttgttggtct
    14761 ggatatcatc ggcgaccgtc tgactgaaat taacgtcacc agcccaacct gtattcgtga
    14821 gattgaagca gagtttccgg tgtcgatcac cggaatgtta atggatgcca tcgaagcacg
    14881 tttacagcag cagtaaccca ccttagcgag aaggatctcg ttgagactct gagtgacagc
    14941 gcccttcttt ccacgcatac tgggcgctgt tgcttttttg aaccaggaaa cagaacctct
    15001 gacaatgaat ttacagcatc actttcttat tgccatgcct gctctccagg atccgatttt
    15061 ccgtcgttcc gtggtctaca tttgcgaaca taataccaat ggtgcaatgg ggatcatcgt
    15121 caacaagccg ctggaaaatc tcaaaattga agggattctg gaaaagctga agatcacgcc
    15181 ggagccgcgt gatgaatcaa tccgtctgga taaaccggtt atgctcggcg gtccgctggc
    15241 tgaagatcgc gggtttattt tgcatactcc gccctccaat tttgcttcca gcattcgcat
    15301 ttcagacaac acagtgatga ccacttcccg cgatgtgctg gaaacgctcg gcaccgataa
    15361 acaaccgtct gacgtattgg tggctctagg ttatgcctcc tgggaaaaag gtcaactgga
    15421 acaagaaatt ctcgataacg cgtggctaac ggccccggcg gatctgaata ttctgttcaa
    15481 aacgccgatt gccgaccgct ggcgcgaggc ggcaaaactg attggtgtgg atattctcac
    15541 catgcctggt gtggcagggc acgcctgatg agtggaacct tactcgcctt cgacttcggc
    15601 accaaaagca ttggcgtagc ggtcggccaa cgcattaccg gcactgctcg ccctttgcct
    15661 gcaattaaag cacaggacgg tacgccggac tggaacctca tcgaacgttt actgaaagag
    15721 tggcagccgg acgaaatcat cgtcggtttg ccgctgaata tggacggcac cgagcaacca
    15781 ttgactgcca gagcgcgtaa atttgccaac cgtattcatg gtcgtttcgg tgttgaagta
    15841 aagctccatg acgaacgtct tagcaccgtg gaagcccgtt ccggtctgtt tgaacagggc
    15901 ggctatcggg cgctcaacaa aggcaaagtc gactctgcct ctgcggttat tattctcgaa
    15961 agctatttcg agcagggata ttaaggcgat ttaaaagcct ggcggatagt cgttaccgcc
    16021 atcttgccgg atgcggcgtg aacgcctatc cggcctacat gtggcagcga ttgtaggtct
    16081 gataagacgc gcaaacgtct catcagacgt ttattgcctt tgtaggcccg ataagcttgc
    16141 acatcgggca tggcaacgtc acaaacgccc ttcccccact cgctgctgaa aactctgctg
    16201 aaacgttatc atccccacct gctgcccggt ttgaataaca tgcggtaact ggtgggtttt
    16261 cccttcgcga atcaaattcc ccaccgcggg agtgttaatc aataattcaa acagcgccac
    16321 gcgtccttcc tgtttatcca cttccagctt ttgtgacaac acggcccgta aactacctgc
    16381 cagttgatta cgtacgggat ctttttcctg cgccggaaat gaatccacca gtcgctcaac
    16441 tgcctgcgcg gcaccacgcg tatgtaatgt tgccagcacc aaatgcccgg tttctgccgc
    16501 cgtcagtgcc agacggattg tctcgctgtc acgcagctct ccgagcaaaa tcacatcagg
    16561 atcttcccgc aatgcggccc gcaatcccga tgcgaacgtc atacagtgca aaccaatttc
    16621 ccgcttctgg atcaaacatc gctggctggc atagatatat tcaacaggat cttccagcgt
    16681 cagaatatgc gcatcggcat gttgattgag atagccaacc atcgccgcca gcgtggtaga
    16741 tttgctgctc cccgtcgccc ccgtcaccag aatcaggcca ttctcactct tgagtaattc
    16801 cggcaatacc gttggtgcgc caagctgttc gagctgcggg cagtgcgaag gtaacagccg
    16861 taacgccagc gaaatgccat gccgttgcgc aaatgcgctg ccacgcaatc gctggttttc
    16921 cgccagcgac acggcaaaat ccagctggcc attttccagc aatattgccc gctgatcgtc
    16981 atccagccac tcccgcagta gctcttcgac gtccggcgcc tcaaacggcg cagcttccat
    17041 tagcccgcga atgcgccatc gtgcgggcca ggcgctgcac aggtgtagat ccgagacgtt
    17101 atgctttaca ctaagggcca caatttcttc catattcata ctaagatcct cggaaaatga
    17161 acgatattgc gcataacctg gcacaggtcc gggacaaaat ctcagcggct gcaacgcgtt
    17221 gcggccgttc tccagaagaa attacgctgc ttgcagtcag taaaacaaaa cctgcgagcg
    17281 ccatcgcaga agccattgat gccgggcagc gtcaatttgg tgaaaactac gttcaggaag
    17341 gggtagataa aattcgccac tttcaggaac tgggcgtaac aggattagaa tggcatttta
    17401 ttggcccgtt gcagtctaat aaaagccgcc tggtggcaga gcatttcgac tggtgtcata
    17461 ccatcgaccg tttgcgcatt gctacccgcc tcaacgatca gcgcccggca gaacttcccc
    17521 ctcttaacgt actgattcaa attaatatta gtgatgaaaa cagtaagtcc gggattcaac
    17581 tggcagaact ggacgagctg gcagcggcgg tcgctgaact accgcgttta cgtctgcgcg
    17641 ggctgatggc aatccctgcg cctgagtcag aatatgtaag gcagtttgaa gttgcacgcc
    17701 aaatggctgt agcatttgcc ggactgaaaa cgcgctaccc gcatatcgac acgctctctc
    17761 tgggaatgtc ggacgatatg gaagccgcca ttgcggcagg tagcacgatg gttcgtatcg
    17821 gcactgcaat ttttggtgcg cgtgattact ctaaaaaata aggaattaaa ggaacgccat
    17881 gaatacgttg actttcctgc tttcaacggt cattgagctg tataccatgg tgctgttatt
    17941 acgcatctgg atgcagtggg ctcattgtga tttttacaac cccttctcac agtttgtagt
    18001 gaaggtaacg cagccaatta tcgggccact gcgccgcgtt attccggcaa tgggccccat
    18061 tgacagcgcc tcgctgctgg ttgcctatat tctcagtttt atcaaagcca tcgtgctgtt
    18121 taaagtggtg accttcctgc caatcatctg gattgctggt ttactgattc tgctgaaaac
    18181 catcggcctg ctgattttct gggtcctgct ggtgatggcg attatgagct gggtaagcca
    18241 gggtcgtagt ccgattgaat acgtgctgat tcagctggcc gatccgctgc tgcgcccgat
    18301 tcgccgcctg ctaccggcaa tgggtgggat tgatttctcg ccgatgatcc tcgttctgct
    18361 gctgtatgtc atcaatatgg gtgtcgcaga agtattacag gcaaccggaa acatgctgct
    18421 gccggggctg tggatggcgt tatgagtgcc gtaacagtta atgatgacgg tctggtttta
    18481 cggctctata ttcagccgaa agccagccgt gattctattg tcggtttaca tggcgacgaa
    18541 gttaaagtcg ccattaccgc gccgccggtt gacggccagg gcaacagtca tctggtgaag
    18601 tttctcggta agcaattccg ggttgccaaa agccaggtgg tgattgaaaa aggcgaactt
    18661 ggccgccaca aacaaattaa aatcattaat ccgcaacaaa tcccgccaga aatcgtggcg
    18721 ttaattaatt aggtatccta tgcaaaaagt tgtcctcgca accggcaatg ccggtaaagt
    18781 gcgtgagctg gcgtcgctgc ttagcgactt cggtcttgat atcgtggccc aaacagacct
    18841 cggcgtagat tccgctgaag aaaccggcct gacctttatc gaaaacgcga ttctgaaagc
    18901 gcgctatgcg gcaaaagtga ccggtttacc ggcaattgcc gacgactctg gtctggcggt
    18961 agatgcgctg ggtggcgcgc cgggtattta ctccgcgcgt tattccggtg aagacgcgac
    19021 cgatcaaaag aatctgcaaa aactgttgga aacactgaaa gacgtaccgg acgaccaacg
    19081 tcaggcgcgt ttccactgcg tgctggtata tctgcgtcac gcggaagatc cgactccgct
    19141 ggtatgccac ggcagctggc cgggcgtgat tactcgcgaa ccagcgggca ctggtggctt
    19201 tggttatgat ccaatcttct tcgtaccttc cgaagggaaa accgccgccg aactgacccg
    19261 cgaagaaaag agcgccattt cccaccgtgg tcaggcgttg aaactgctgc tggacgcttt
    19321 acgtaatggt taaattaccg ccgctgagtc tctacattca catcccgtgg tgcgtgcaga
    19381 aatgcccgta ctgcgatttc aactctcacg cgttgaaagg agaagtgccg cacgacgatt
    19441 atgttcagca tctgctttgc gatctggaca atgatgtggc ttacgctcag ggccgtgaag
    19501 taaagacaat ctttattggc ggtggtacgc cgagcctgct ttccggcccg gcgatgcaaa
    19561 cgctgctgga cggcgtgcgt gcgcgtttgc cgctggcagc ggatgcagaa attactatgg
    19621 aagcgaaccc tggaacggta gaagccgatc gctttgtcga ttatcagcgt gctggtgtga
    19681 accgcatctc tattggtgtg cagagtttta gcgaagaaaa gctgaaacga cttgggcgta
    19741 ttcatggccc gcaagaagcg aaacgcgcgg caaagctggc gagcgggctg gggctgcgta
    19801 gctttaacct cgatttgatg catggactac cggatcaatc actggaagag gcgcttggcg
    19861 atctacgcca ggccattgaa ctgaatccgc cgcatctttc ctggtatcaa ctgaccatcg
    19921 aacccaatac gctgtttggt tcgcgaccac cggtgctgcc ggacgatgac gcgctgtggg
    19981 atattttcga acaggggcat cagttattaa ccgcagcggg ttatcagcaa tatgaaactt
    20041 ccgcttacgc caaacccggt tatcagtgcc agcacaatct caactactgg cgatttggtg
    20101 actacatcgg tattggctgc ggcgcgcacg gcaaagtcac cttcccggat gggcgcattc
    20161 tgcgtaccac taaaacgcgt catcctcgtg gttttatgca aggaaggtat ctggaaagcc
    20221 agcgtgatgt cgaagccgca gataagccgt ttgagttctt tatgaatcgc ttccgtctgc
    20281 tggaggccgc gccgcgcgtg gagtttattg cgtataccgg gctttgcgaa gatgtgattc
    20341 gcccacagtt agacgaggcg attgcccagg gttatctcac cgaatgtgcg gattactggc
    20401 agataaccga gcacggtaaa ttattcttaa actcacttct tgaattattc ctcgctgagt
    20461 aatcattaat gcgcctgcgg gcgcattaat cacttctttc ccccccgtta attaacttcg
    20521 gcaaaatata tttcttactg aataaacatc gatgatattt attagatata tctagtgaat
    20581 atttactcat catttattga ttataaacat gtacaacctg gcgcacaaat ttaatattta
    20641 atttctgact ttatttcaag ttgtgataaa catagacaag ttgtcagata ggttaaccaa
    20701 tttgtcgcac aaagcactga taagcacaat tagatatcaa ccaatttcca tccttcaaca
    20761 gggagttttt ttatgaaaat gaggtcattt acccgctcat tagtctgcgc atccttgctg
    20821 gctctggtta gcactggcgt aaacgccgca gaaaaagtga cgttaaagtt agcacacaac
    20881 ctggaaagaa gtcatgttgt acatcagagt tttgaagagc tggcgaaaga ggtaaaacag
    20941 ctgtcagaag gtaatatggt gatccgtatt tatcccagca gccagatggg taatgcgcga
    21001 gaaactatgg aattgttgca aaatggcgca ctggatatga ccaaaggttc cgccagtgat
    21061 ctggagtctt ttgataatat ttatgctatc tataatttgc cgttcttatt taaagatcag
    21121 gctcacttca acaaagttgt ttttggtgaa gttggtaaag aaattatgga ctcaacaaaa
    21181 gacaaaggct tttttgcttt atcagcttat gtagcgggta cacgcagttt ctatgcgaaa
    21241 aaaccgatta ctaagccaga agatcttaaa gggctgaaaa ttcgtgttca gccaagtccg
    21301 accaccatca aaatgattga actgatgggt ggttcaccca ctccaatttc atttggcgaa
    21361 gtttatactg cgatgcagca aggtgtagta gatggcgcag aaaataacgt gccttcctgg
    21421 gtgcagactc gacatattga aattgccaaa gttttttctg aagatgaaca cgcctcaatt
    21481 cctgactttt tggttatctc caccaaaaca tggaataaat taaccccaga acagaaacaa
    21541 atcctcgaaa cagcagcgaa gaaatccgaa gcttatcaac aaaaattgtg ggaaaaaatt
    21601 gacgctgaca cacgtgctca ggcgaaagct atggggggtg aaatt
//
LOCUS       DAJVRF010000062        21643 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJVRF010000062 DAJVRF010000000
VERSION     DAJVRF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.062"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="OQN13_002147"
     CDS             complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="OQN13_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCS1615503.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPRSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1345..2997
                     /gene="ushA"
                     /locus_tag="OQN13_002148"
     CDS             1345..2997
                     /gene="ushA"
                     /locus_tag="OQN13_002148"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCS1615504.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTREDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="OQN13_002149"
     CDS             complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="OQN13_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCS1615505.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3717..4511)
                     /locus_tag="OQN13_002150"
     CDS             complement(3717..4511)
                     /locus_tag="OQN13_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCS1615506.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4649..4990
                     /locus_tag="OQN13_002151"
     CDS             4649..4990
                     /locus_tag="OQN13_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS1615507.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5205..7709)
                     /gene="copA"
                     /locus_tag="OQN13_002152"
     CDS             complement(5205..7709)
                     /gene="copA"
                     /locus_tag="OQN13_002152"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCS1615508.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTIVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7971..8903
                     /gene="glsA"
                     /locus_tag="OQN13_002153"
     CDS             7971..8903
                     /gene="glsA"
                     /locus_tag="OQN13_002153"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCS1615509.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8906..10198
                     /locus_tag="OQN13_002154"
     CDS             8906..10198
                     /locus_tag="OQN13_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1615510.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10323..10730
                     /gene="cueR"
                     /locus_tag="OQN13_002155"
     CDS             10323..10730
                     /gene="cueR"
                     /locus_tag="OQN13_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCS1615511.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10731..11189)
                     /locus_tag="OQN13_002156"
     CDS             complement(10731..11189)
                     /locus_tag="OQN13_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCS1615512.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11186..12103)
                     /locus_tag="OQN13_002157"
     CDS             complement(11186..12103)
                     /locus_tag="OQN13_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCS1615513.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12249..12926
                     /gene="fetA"
                     /locus_tag="OQN13_002158"
     CDS             12249..12926
                     /gene="fetA"
                     /locus_tag="OQN13_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCS1615514.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12913..13692
                     /gene="fetB"
                     /locus_tag="OQN13_002159"
     CDS             12913..13692
                     /gene="fetB"
                     /locus_tag="OQN13_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCS1615515.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="OQN13_002160"
     CDS             complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="OQN13_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCS1615516.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="OQN13_002161"
     CDS             complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="OQN13_002161"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS1615517.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="OQN13_002162"
     CDS             complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="OQN13_002162"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCS1615518.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16063..16749
                     /locus_tag="OQN13_002163"
     CDS             16063..16749
                     /locus_tag="OQN13_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1615519.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16746..19160
                     /locus_tag="OQN13_002164"
     CDS             16746..19160
                     /locus_tag="OQN13_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1615520.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="OQN13_002165"
     CDS             complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="OQN13_002165"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCS1615521.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20557..21483)
                     /gene="allS"
                     /locus_tag="OQN13_002166"
                     /pseudo
     CDS             complement(20557..21483)
                     /gene="allS"
                     /locus_tag="OQN13_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5014 a         5684 c         5800 g         5145 t
ORIGIN      
        1 tgatcggcga taagccccag aactgccgct cccagacctc ccatgccaaa agcaaaaccg
       61 aaaaagagtc cagaaaccat accgatacgt cccggaagta gctcctgagc gtagaccaga
      121 atggcagaga atgccgaagc gaggataaat ccaataatca ccgttaaaac ccccgtccag
      181 tgcaggctgg cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat agagccccaa
      241 atcacatatt tccgtccaat tttatcccct acaggcccgc cgatcaccgt acctgccgca
      301 acggcaaaca ggaaggcaaa cagatgaagc tgggcattct ggatagataa tccgaatttt
      361 tgcatcagat aaaaggtgta atagctgctg atgctcgcca tatagaaata tttcgagaaa
      421 atgaggatta acagaatgct gactgccagt accactttgt tgcgcggcag tggattgatt
      481 atcgtcgctt tgggttttcc tttattcatt cggtgctgtg ccgagtacca acggctaatt
      541 tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac gttgcctttg
      601 ccataaggcg cgataatcac cgccgccagc aagggtccca gggaactgcc aaagttgccg
      661 ccgacctgaa agatagattg cgccaggcca tgccgcccgc cggaagccat acgggctacg
      721 cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc cgccagcaga
      781 actgcgccaa aactgcccgc cagcgcaagc agcaccagac cacttaaggt aaagcacatg
      841 ccaattggca acgaccgcgg catcggatat ttatcggtcc agtagccgac cactggttgc
      901 agtagcgaag aggcgagctg gaaggtgagg gttatcatgc caatctgcat aaatgtcaga
      961 gaaaattctg actgaagcag cggataaatc gccagaatca gcgattggat catgtcgttc
     1021 agcagatgtg agaggctgat agcacctaaa ataccaaacg atgttcgggc cttggtcgtt
     1081 gacgcagccg cgcccgccac aggctggggt tgttcactca ttgccatagg aaagtcactt
     1141 tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt attacccgac taacatacct
     1201 gtatgcgtcg tctgaaggaa gtctcaacgc cgaatacaga atttctaatc tggatgcaga
     1261 tttatcttca ccggacgcag acttgtctat gatgtcgcgt catactattt ttcaacacgt
     1321 tgaaatcagg tcagggagag aagtatgaaa ttattgcagc ggggcgtggc gttagcgctg
     1381 ttaaccacat ttacactggc gagtgaaact gctctggcgt atgagcagga taaaacctac
     1441 aaaattacag ttctgcatac caatgatcat catgggcatt tttggcgcaa tgaatatggc
     1501 gaatatggtc tggcggcgca aaaaacgctg gtggatggta tccgcaaaga ggttgcggct
     1561 gaaggcggta gcgtgctgct actttccggt ggcgacatta acactggcgt gcccgagtct
     1621 gacttacagg atgccgaacc tgattttcgc ggtatgaatc tggtgggcta tgacgcgatg
     1681 gcgatcggta atcatgaatt tgataatccg ctcaccgtat tacgccagca ggaaaagtgg
     1741 gccaagttcc cgttgctttc cgcgaatatc taccagaaaa gtactggcga gcgcctgttt
     1801 aaaccgtggg cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg gctgacaacc
     1861 gatgacacag caaaaattgg taacccggaa tacttcactg atatcgaatt tcgtaagccc
     1921 gccgatgaag cgaagctggt gattcaggag ctgcaacaga cagaaaagcc agacattatt
     1981 atcgcggcga cccatatggg gcattacgat aatggtgagc acggctctaa cgcaccgggc
     2041 gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt cggtggtcac
     2101 tcgcaagatc cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta cgtgccgggt
     2161 acgccatgca aaccagatca acaaaacggc atctggattg tgcaggcgca tgagtggggc
     2221 aaatacgtgg gacgggctga ttttgagttt cgtaatggcg aaatgaaaat ggttaactac
     2281 cagctgattc cggtgaacct gaagaagaaa gtgaccaggg aagacgggaa aagcgagcgc
     2341 gtgctgtaca cccctgaaat cgctgaaaac cagcaaatga tctcgctgtt atcgccgttc
     2401 cagaacaaag gcaaagcgca gttggaagtg aaaataggcg aaaccaatgg tcgtctggaa
     2461 ggcgatcgtg acaaagtgcg ttttgtacag accaatatgg ggcggttgat tctggcagcc
     2521 caaatggatc gcactggtgc cgactttgcg gtgatgagtg gaggaggaat tcgtgattct
     2581 atcgaagcag gcgatatcag ctataaaaac gtactgaaag tgcagccatt cggcaatgtg
     2641 gtggtgtatg ccgacatgat cggtaaagag gtgattgatt acctgaccgc cgtcgcacaa
     2701 atgaagccag attcaggtgc ctacccgcaa tttgccaacg ttagctttgt ggcgaaagac
     2761 ggcaaactca acgacctgaa aatcaaaggc gaaccggtcg atccggcgaa aacttaccgt
     2821 atggcgacat taaacttcaa tgccaccggc ggtgatggct atccgcgcct tgataacaaa
     2881 ccgggctatg tgaataccgg ctttattgat gccgaagtgc tgaaagcgta tatccagaaa
     2941 agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg gcagtaatcc
     3001 gaaagtgccg gatatttgca tccggcacaa tgcttaatcg cggcgggcga tatcagcaaa
     3061 tttggcatcg aggatcttcg ccagatcgcc tgccgccagt tcgatatcca gtccgcgctt
     3121 gccgccagaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa tcgtcggcag
     3181 acgttttttc tgccccaatg ggctaatccc cccaaccagg tatcccgtcg aacgctgcgc
     3241 ggccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta cttttttaag
     3301 atccagttga cctgcgaccg gcgtaacggc cactgccaga tgtttcatat caccgttcac
     3361 tgccaccagt agcgttttgt agacttgatc cggattcaaa cctaattttt tgacgacttc
     3421 atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa acgaaatctt
     3481 gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt aaacaaaaaa
     3541 acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat
     3601 tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag tgacggcata
     3661 ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc atttttttag
     3721 cgcaacattt gcggcaaatt cccttctcca tacaggtgta gtgcaccgac cgcgaccaca
     3781 tatcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg ctgatgcatc
     3841 agcacatcgt acagcgactg actgaacgta ttgggcagcg ttatatcatt attttgcgga
     3901 ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt atgccagtgg
     3961 gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcaa catggtaatc
     4021 tggttttcag ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc cgcctgcaat
     4081 aactggtaat cgataccgta ttctgcccgc agccccagtt tttgtgcctg cgtcgcctga
     4141 agaaccatcg cgatttgcca cagcggttga gtagaaaaga gcgacggaga aatgcccatc
     4201 tcctggctaa tgtgctgcag attttggagt tgctcctcgt taatgcgctc ttccagcgcc
     4261 tcgcaagtag gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc
     4321 agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc catatcgtgg
     4381 ctacccatat gaatactgcc aatcagatga aaatggcgat tgccgggaag ggtgatatcg
     4441 atggcaggcc aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt tacccggtac
     4501 aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg agggatggcg
     4561 caaatggagt tggggacttg tttaatgtgt ttgtatgatt cagtatgttc ttgcatcgct
     4621 attcacaggg aagcaacagt taaaaaccat gaaacaggca acaagaaaac cgacgacacc
     4681 cggagatatt cttctctatg aatatctgga accgctcgat ttgaaaatca atgagttagc
     4741 agagttgctg catgttcatc gtaatagcgt cagtgcactg atcaataaca atcgtaaact
     4801 caccactgag atggcattcc gtctggcgaa agtttttgat accacagtcg attttaggct
     4861 aaacctccag gcggcagttg atctttggga agttgaaaac aacatgcgca cccaggaaga
     4921 attgggacgg attgaaacag tggctgaata tttggcacgc cgtgaagagc gtgcaaaaaa
     4981 ggtcgcgtaa ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt tcacgccgca
     5041 tccggcatcc gagcaccatt gcctgatgcg acgcttaccc gtcttatcag gcctacaaac
     5101 ctgtgcctga accgtaggcc ggataaggcg tttacgccgc atccggcagt catgcgtcga
     5161 tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc cttcggttta
     5221 aaccgcagca accggttggc gttactcact acggtaatcg acgagagcgc cattgccgct
     5281 ccggcaacta ccgggttaag cagtgttccc gtaaacggcc acaaaatacc ggcggcgacc
     5341 ggaataccga tgctgttgta gataaaggca ccgagcaggt tctgcttcat gttgcgcagc
     5401 gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg catcagggta
     5461 atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc gacatccgcc
     5521 tgggccagcg ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg acgtccttca
     5581 ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcactccggc aatcacttca
     5641 tcaattccag cttctttggc gatcgcattg gcggtggttg ggttatcccc ggtcaacatc
     5701 accagacgat atcccgtttt atgcaggcgt tgcagcgccg ccacgctatc actacgcaac
     5761 ggatcgcgta cagccagcag ggctactgct ttcccgtcaa tcgccagcag cacaggcgtt
     5821 gctccttgcg atgcctgagc agtaatctcc gcttcgatag ctttggtacc aacctgttgc
     5881 tcatttaaca gcgcctgatt gcccagcaat aacgcatgac cttcagcttc accgctcacg
     5941 cccagcccgc gcaatgtgcg gaaaccgttg acctgcggca gctgcatatc acctgcttta
     6001 tcgaggatcg cccgtgccag cggatggctg gaaccttgct ccagtgccgc cgccagacgc
     6061 aatgcctgcg cttcatcaac atcagcaaac gttttcactg cgacaacctg cggcttccct
     6121 tcggtcagcg tcccggtttt atcgaacacg atggtatcga gtgtactggc gcgttgcagc
     6181 gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc ggaaataatc
     6241 gacatcggcg tcgccagccc cagcgcacac ggacaggcga taatcagtac cgtggtggca
     6301 atcaccaggg tataaacaat ttgcggtgcc ggaccaaaga agtaccagat tgccgcactg
     6361 accagcgcaa tcaccaccac cacaggtaca aatacggctg agattttatc cgccagctga
     6421 ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatccg tgacagcgtg
     6481 gtatggctgc caaccgcact ggcacgaaac agcacactgc cgtcctgtac cactgtcccg
     6541 gcatggacgc tttcgccttc gcctttttgc tgtgggattg gttcgcccgt cagcatcgct
     6601 tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc
     6661 gtcaggcgca gtaacatccc tggctgaact tccgccagcg gcacgctttt ttcaccatct
     6721 gcggtgacca ctcgcgccgt cggtggggtt aaatcgagta atttctccag cgccttagaa
     6781 gaacgctgac gtgcgcgcgc ttccagcata tggccgagat taatcagacc gataatcatc
     6841 gcactggctt catagtaaag atgccgcgct tccatcggga accactgcgg ccacaggttg
     6901 acgctcatcg aatagagcca cgccacaccc gttcccagcg ccaccagcgt atccatcgtc
     6961 gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgcc ggcgaaaacc
     7021 atcactgcca gggttatcag gccgataact aaccacaagc tgcggttgtc agcggtgacc
     7081 atcatgttat cgccgatcat cccccagacc attaccggga tacccacagc cagtgcaaca
     7141 attgcctgcc agcggaagcg cttcatcgta gctacggcgg tttcttgctg gcgctcgcgg
     7201 cgtttagcgt catcttcaat cgcttccgca ccgtagcccg ctttttccac cgcctgcact
     7261 aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag gtttacccgt
     7321 gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac acagctggcg
     7381 cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt cgctgccgga
     7441 agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc cagcggttta
     7501 gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt
     7561 tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc ctgctcaaca
     7621 tccgggcgct gttcaagact ttctttcacg cgtttaacgc agtgaccgca ggacaggccg
     7681 tccagggtca ggtcgatagt ttgtgacata aaacactcct ttaagacagt tttgactggc
     7741 tgtgataaag gttaaacctt ccagcaaggg gaaggtcaag aaattaataa accaggcggg
     7801 taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga catttatttc
     7861 tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct gaaaatcggc
     7921 accggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag atgttagatg
     7981 caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca cttaacggcg
     8041 gacaaaatgc cgattacatt ccctttctgg cgaatgtacc aggtcaactg gcggcagtgg
     8101 ctatcgtgac ctgcgatggc aacgtctata gtgcgggtga cagtgattac cgctttgcac
     8161 tggaatccat ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg
     8221 cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca gttatcgcct
     8281 tagagttgca tggcggcaaa ccgctgtcgc cactggtaaa tgctggcgct attgccacca
     8341 ccagcctgat taacgctgaa aatgttgaac aacgctggca gcgaatttta catatccaac
     8401 agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa
     8461 ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat ctctattgtg
     8521 atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc aatactgttg
     8581 aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg acgcataaac
     8641 gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg gaagggctgt
     8701 atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg
     8761 gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca ccaccgctgg
     8821 acgaagaagg caacagtgtt cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg
     8881 gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg gtaacaaacc
     8941 cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg cggggatctt
     9001 cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctgggtcg cctttgcttt
     9061 tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgtctggggg cgagctatcc
     9121 cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg tcttttcgct
     9181 agcgctctcg ttgctgtacc tgttgacgct ggcggtgagc atcgccatgg ttgcccgtgc
     9241 ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc accttatttt
     9301 gctctacgcg ttggggatca ttgcggtgat gacgcttttt aactccttaa gcaaccatgc
     9361 ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt tattgctgat
     9421 tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc cccccagctc
     9481 cggtgcgttc ttctcctgta ttgggataac cttccttgcc tatgcgggct ttggcatgat
     9541 ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg cgtttctggt
     9601 ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc ttagcgatgt
     9661 atcggcatta gagttagaaa aatatgctga taccgccgta gcgcaggctg cttctccgct
     9721 gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga cggcttcagc
     9781 cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca gcgaacgcga
     9841 actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca acattattgt
     9901 cgtggtgttg attatgctga tgacggcggc gctaaattta ggctcactcg ccagtgttgc
     9961 cagcgccacc tttttgattt gctacctggc ggtgtttgtg gtggcgatcc gcctgcgtca
    10021 tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt tgttggtgat
    10081 cgttggcttt atctacagtc tgtggtccca gggtagccgt gcgttgatat ggattattgg
    10141 ctcactctta ctcagcctta ttgtggcaat ggtcatgaag cgcaataaaa ccgtataaca
    10201 tctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc gcactggcaa
    10261 aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa
    10321 cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc attcgcttct
    10381 atgaagagaa ggggctggtg acgccgccga tgcgcagcga aaacggttat cgcacctaca
    10441 cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg ggctttaacc
    10501 tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg
    10561 tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag gagctgcaat
    10621 ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac agcgccgact
    10681 gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga ttaagacgag
    10741 acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacatg cgtacctgca
    10801 ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat atgaccgcgc
    10861 ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg
    10921 tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca tttccaccac
    10981 agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc ccaacccagc
    11041 ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca caacaaataa
    11101 ccatttccgc ccagcatctc ggctgccagc agcaaaccgc cgagactgag ccagaaaata
    11161 tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg ctgtctttca
    11221 ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat ggcatcatca
    11281 ctactttgct gttactggag gtaccgatct gctgtaacgc ttcggtgtat ttctgcgcta
    11341 cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc attttggtgg
    11401 cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac
    11461 gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg aggatttccg
    11521 cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct
    11581 gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg
    11641 tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg cgtgagttga
    11701 tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg gtacggatgt
    11761 tagtcatggt caggttgatg atcgccagct ccagattgct gacttcataa gccgcgcgcg
    11821 gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg ttatctttcg
    11881 agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc ttgcgaccaa
    11941 tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg gtatagcgac
    12001 caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc gcgccgacaa
    12061 tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa acctcctgtt
    12121 gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct gattactgaa
    12181 agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg
    12241 gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga tatctggcgg
    12301 gtgatgcgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa
    12361 ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct tcattgatca
    12421 gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta aagccagaaa
    12481 tctaccgcca acaagtctct tactgcgccc agacaccgac gctgtttggc gatacggtat
    12541 acgataatct gatctttccc tggcagatcc gtaaccggca gcctgaccca gccatttttc
    12601 tcgattttct cgaacgcttc gccttgccgg acagcatttt gacgaaaaat atcgccgagc
    12661 tatctggtgg tgaaaaacaa cgcatctcac tgattcgtaa cctgcaattt atgccgaagg
    12721 ttttattgct ggatgaaatc accagtgcgc tggatgaaag taataaacat aacgttaatg
    12781 agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg acacacgata
    12841 aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc ggagaaatgc
    12901 aggaagcacg ctatgaactc gcataatatt actaacgaat cattagcact ggcattaatg
    12961 ctggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga gaaagatatt
    13021 ctctggagcg tcggacgagc gataattcag ctgattattg tcggctatgt gctgaagtat
    13081 attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat ctgctttaat
    13141 gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat ctcatcgttt
    13201 attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc agggtcgatt
    13261 gaatttatcc cgatgcaggt gatccctatc gccgggatga ttgccggtaa cgccatggta
    13321 gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca gcaacagatt
    13381 caggagaaac tgagtcttgg tgcgacgccg aagcaggctt cagcgatatt gattcgcgac
    13441 agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg cttagtgagt
    13501 ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa ggcgattaaa
    13561 tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac cattattgcc
    13621 tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt gacgcaattg
    13681 aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc
    13741 cgatcaacgg aatatcaata caacaatgca tacagctggc ggcgatactt cgacgccagt
    13801 gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct
    13861 gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc ttcattgcgc
    13921 ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc tggattctcc
    13981 gccacctgct gttgcaactg ttgaatttcc ggcgtatcag ccgcctgctt cagcagttcg
    14041 atttgcgcca ccatcccctg gtagcgggtg tcctgatcct gtaacggaat ggttttcagc
    14101 accgcttctg catcttcaga acggttcagc gcaatcagcg tttctgccaa cagcaggccg
    14161 atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag ggcatcggtg
    14221 aaattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc
    14281 agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg gaagccatct
    14341 accggttgcc cgttctggaa cagatacacc gtcggaatcg cgcgcagacc aaactgggcg
    14401 gcaatcatct gctccgcatc gcagtccagc ttcgccagaa taaattgccc gttgtactgc
    14461 gccgcaaggc tttccagaat tggggttaac tgcaaacagt gctggctacg ttcagaccaa
    14521 aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag gttagattcg
    14581 ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga tattttcttt
    14641 gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc catcacgcgc
    14701 cccggcagca ggcgcttaag caccattact gcccaagtca ccagcgttac cggatagcgc
    14761 atcttcggct tctcgctaat aaaagcatgg cgtactttgt ccaccaccgc ttccggtccc
    14821 aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg cgtctggttg
    14881 acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac tttaattccg
    14941 ctgtggcgta gctccatgcg cagcgcgtct gaccatgcct ccagcgcata tttactggcc
    15001 gcgtaagcgc cacgacccgg tgtggagatt aatcccatca ccgatgatgt catcacaata
    15061 cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg
    15121 aaaaagttgg cggaaaactg ctgttccatc tgcgcgcggc tgatggtgga aagggggcca
    15181 tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt cagggcgatc
    15241 acttcgtcgg ctgcgcgatc aacactttct ggcgaatcca gatcgatcaa cacgccggta
    15301 aatcccatgc tgttcatacg ctcaacatca tccggtttcc ggcaacctgc cagcacatga
    15361 aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga acatccggta
    15421 attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt atgagtcatg
    15481 atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct gggcgtcgcg
    15541 gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct cttccataaa
    15601 aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc
    15661 ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca atggttcagc
    15721 gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg
    15781 aaaaccacgc aaaccgtcat tgccgcccag ttcaaccagc acccaacgcg gctgatgctg
    15841 tttcagcaga gccggaaggc gcgccagtcc ttgttgcgag gtgtcgccgc tgatgctggc
    15901 attaactacc gacgttttac tctgccactt atcattcaac aaggcaggcc aggccgcgct
    15961 ggcagacatt cgatacccgg cgctcaggct atcacccaga atcaataacg tgtccgctgc
    16021 ggcggcacgg aaggttaaca gggccaggaa caggaagggc aaatgccagc ggaaaacatt
    16081 gttgaagttc atcatcttaa gaagtccgtc ggtcaggggg agcatgaact ctccatcctc
    16141 accggagttg agctggttgt caaacgtggc gagaccatcg cactggtggg cgagtcggga
    16201 tcgggtaagt caaccttgct ggcgatcctc gccgggcttg atgacggcag cagtggcgaa
    16261 gtgagtctgg tgggacaacc gctacataat atggacgaag aagcgcgggc aaagttgcgc
    16321 gcgaagcacg tcggctttgt ttttcagtca tttatgttaa ttcctaccct taacgcgctg
    16381 gaaaacgtcg agcttccggc tctgctgcgc ggtgagagta gcgcggaaag tcgtaacggg
    16441 gcgaaagcgt tgctcgaaca gttagggctg ggtaaacgtc tggatcatct tccggcacag
    16501 ctttccggcg gtgaacagca acgagtggcg ctggcacgag cctttaatgg tcgacctgat
    16561 gtgctgtttg ccgacgaacc caccggcaac cttgaccgcc agacgggcga taaaattgcc
    16621 gacctgctgt tttccctcaa ccgtgaacat ggcaccacgt tgattatggt gacccacgac
    16681 ctgcaactgg cggcacgctg cgaccgctgc ttacggctgg tgaacgggca gttgcaggag
    16741 gaagcatgat tgcacgttgg ttctggcgcg aatggcgttc gccgtcgcta ttaattgtct
    16801 ggctggcgct aagcctggcg gtggcctgcg tgctggcgct gggcaatatc agcgatcgca
    16861 tggagaaggg cttaagccag caaagccgtg agtttatggc gggcgatcgg gcgttgcgca
    16921 gttcacgcga agtgccgcaa gcgtggctgg aggaagcgca aaagcgcggc ctgaaagtcg
    16981 gcaagcagct gactttcgcc acaatgacct ttgcaggcga cacaccgcag ctggcgaacg
    17041 tcaaagcggt ggatgatatc tacccgatgt atggcgatct gcaaactaat ccccctggcc
    17101 tgaaaccgca ggcgggcagc gtattgctgg ccccacgcct gatggcactg cttaacctga
    17161 aaacgggcga caccattgac gtgggcgatg ccaccttgcg gattgccgga gaagtgattc
    17221 aggaaccgga ttccggtttt aaccccttcc agatggctcc gcgtctgatg atgaatctgg
    17281 cggatgtcga taaaaccgga gccgtgcagc cggggagtcg ggtcacctgg cgttataaat
    17341 tcggcggcaa cgagaaccag ctcgacggct atgataaatg gttgttacct cagcttaaac
    17401 ccgaacaacg ctggtacggt ctggaacagg acgaaggcgc gctggggcga tcgatggaac
    17461 gctcgcaaca gttcctgctg ctttcggcgc ttctgacctt gctgctggca gtggcagcgg
    17521 tggcggtagc gatgaatcat tactgtcgca gtcgctacga tctggtggcg atcctcaaaa
    17581 cgctgggggc agggcgagcg caactgcgta agctaatcgt cggtcagtgg ttgatggtgc
    17641 tgacgctttc agccgttacc ggtggggcca taggcctgtt gttcgaaaac gtgttgatgg
    17701 tgctgctcaa gcccgttctg cctgctgcac taccgccagc cagcctctgg ccgtggctgt
    17761 gggcgcttgg caccatgacg gtcatctcgc tgctggtggg gctacgacca taccgcttgt
    17821 tgctggcaac gcagccttta cgcgtattac gtaatgatgt ggtagcgaac gtctggccgc
    17881 tgaagtttta tctgccgatt gtcagtgtgg tggttgtgct gctgctcgcc ggattaatgg
    17941 gtggcagcat gctgctttgg gcggtgctgg cgggcgcggt agtactggct ttgctgtgcg
    18001 gtgtgctggg ctggatgcag ctgaatgtac ttcgccgcat gacgctgaaa tcgctacctc
    18061 tgcgcctggc ggttagccgc ctgttacgtc agccgtggtc aacgttaagc cagctttcgg
    18121 cattttcgct ctcctttatg ctgctggcgc tgttgctggt attgcgtggc gatctgctcg
    18181 accgctggca gcagcagcta ccgccagaaa gcccgaacta ctttttaatt aacatcgcca
    18241 cagaacaggt agcaccgcta aaagcgttcc tcgcggaaca tcatatagtc ccggaatcat
    18301 tttatccggt agtgcgggcg cggctgacgg cgattaacga taagccgaca gaaggtaatg
    18361 aagatgaggc gcttaatcgc gaactcaatc ttacctggca aaatacgcgg cccgatcata
    18421 atccgattgt cgccggtaac tggccgccaa aagccgatga agtgtcgatg gaagaggggc
    18481 tggcgaaacg cttaaacgtt gccctcggtg ataccgtgac ttttatgggc gatacccagg
    18541 agttccgcgc taaagtgacc agcctgcgca aagtggactg ggaaagtctg cggcctaatt
    18601 tctattttat tttcccggaa ggggcattag acgggcaacc gcagagctgg cttaccagtt
    18661 tccgctggga gaatggcaac ggcatgttga cgcaactcaa ccgccagttc ccgaccatta
    18721 gcctgttaga tattggcgcg attttaaaac aggtcggtca ggtgctggag caggtaagtc
    18781 gggcgctgga ggtgatggtg gtgctggtca ccgcctgcgg tatgttgctg ttgctggcac
    18841 aggtgcaggt gggaatgcgt cagcgtcatc aggagctggt ggtgtggcgc acactcggtg
    18901 cggggaaaaa actgctgcgt accacgttgt ggtgtgagtt cgccatgctt gggtttgttt
    18961 ctggcctggt agccgcaatt ggtgcggaaa cggcgctggc agtgttgcag gcgaaagtgt
    19021 ttgatttccc gtgggagcca gactggcgat tgtggattgt tctgccgtgc agcggtgcac
    19081 tgctgctgtc gcttttcggc ggctggctgg gtgcgcgact ggttaagggt aaggcgctgt
    19141 tcaggcagtt tgcggggtga tgacaggtgt tagattgcct gatgcgctac gcttatcagg
    19201 cctacgaaga gcgtgcaata tattgatttt gcaagattgt gtaggccgga taaggcgttt
    19261 acgccgcatc tggcatgaac agtgtgcatt ttggcaataa atatgaaacc aactgtcatg
    19321 tcggtttttt atatctgtat tctggtgcaa ccattctgtt ctcacgacaa tcgccgtttc
    19381 agaataccgc ccatcactgc gtcttaaccc attccgccac ttccgcccac tcaccgcgaa
    19441 agacaacttt ttccgctttt ttctcaagct gatagcgata catcgggtcg taatattctt
    19501 taagtaacgg caccagccag gccagatgac cgtcggtgct gtcggtggtg agttgcgttg
    19561 tcagcgccgt atccagctgg gcagcgagtt cgttatagcg ctgtaacccc agccgacgct
    19621 taatagccga aagtccgtga tgcaggtatt cgcaatattc ctgccagccc tgttcgtcgc
    19681 cgtacgcgtg ggtaaaatca tgatgcatac gcaagaaata ctcttcgttc aggcgctcaa
    19741 gacggatctc aaacggatct tctaccaccg caatcgccgc ctgagtcatt cgctcgcgca
    19801 ggcattccgg caggtgattc gaaccgatca tccggctttc gtcttccagc acccacaggc
    19861 gcaaatcctg acgggcgtcg gtttttagca tttcggcagc cagcaggttt tcaaaactcg
    19921 cctggctaag ttgtggttgt aaggtgcgac caaacgccga accgcgatga cgcgctaacc
    19981 cttccagatc aacaccgttc ggctgttgct gcactaacag cgttttaccg ctgccggtac
    20041 aaccgccaat cagcactatc ggtttttgtg ccagttcaat agtcgcctga atcgcggctt
    20101 ggcgcagtgc cttataaccg ccttccacca gcggataatc aatccccgct gcatacaacc
    20161 agctttgcac aatatgtgag cgctgaccgc cacgggcgca gcagagaata ccttgcggat
    20221 tttgcaggca cgctgcccgc caggcgtcca tgcgctgctg acgaatttca cccgccacca
    20281 gtttatgccc cagcgccagc gccgcgtctg agccttgctg tttataacaa gtgccaacgg
    20341 cggcgcgttc atcgttattc attaacggca ggttgatagc ggcgggcatt gcgccttgct
    20401 caaactcgat aggggcgcga acatcaataa tgggcgtatc agcaatcagc agggcacgat
    20461 agtcctgttc cgtgtgtctc tcttgcatag ttaaaagtga acctcaaatc agcttgcgcg
    20521 ctattttacg cgccaacgcg caaggaaact tgatttttaa ctgcgtgggt tgccgaaaat
    20581 ttctaaaaat ccgctgattt ccggcctgcg ctgggtaaac aaggtcacaa tatcttctac
    20641 cgctttgccg ctgccaaatt tgcgccatgc cagactcaat ggcgaaggag ggcgcatcgt
    20701 tgggattacc cggctgacca gttgttgatt atcgatcatt gactggcaaa gtgattttgg
    20761 caaaaaacca atgccaacac ccgccagatg ggcggcgatt ttcgtttcca tatcgggaac
    20821 aataatctct ttttgccccg gcaatcgcca ggcgacgcgt ttggttaagg ttcgggcgct
    20881 gtcttcaata ttgaccgccg gaaagcgccg caactgcgct tctgttagcg gctcttcaac
    20941 gttcgccagc ggatgatccg ccgccatgac aaagcgccat tgcaccgatc ctaagggatc
    21001 aagactaaag gtatttgcca gcgcctcagt tcccgtgacc ccgatagcca gcgaaaaacc
    21061 ttcgtacaat agcgagtccc agacgcccat atagatttgt cgggagatgt gaaactgggt
    21121 aaaggggtaa cgctcattca gccacgccag caactgggcg acggcctggg ggttatagag
    21181 caggttgttg atgacaatat tcacctagcg ttccacgcca tcattcacct gttgcagctc
    21241 gctgggcata ctttccagcc agctcagcca gtctctggcc tgggaaagta gatgctcgcc
    21301 agccgctgtc aacgtcacgc tgcgagtcgt gcggaaaaac agcgccactc cggtattctc
    21361 ttccagaagt ttaatgcgat aactgatcgt cgccgtggtt ttacataatc gttctgccgc
    21421 ttttgaaaaa cttcctgttt cagcaaccgc aatgaaagtc cgcaaggttt ctggatcgaa
    21481 catcttcagg tatccccttt taaatccgca agttgcgtga ttttcttatc ctctgattta
    21541 tcagtatttt tacatgataa ccctgttcaa tttgtggact aaatctagtt ttggaaaaat
    21601 attccaactt ttgtattgat gttgttctct taaggtttta gat
//
LOCUS       DAJVRF010000063        21135 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJVRF010000063 DAJVRF010000000
VERSION     DAJVRF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21135
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.063"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(11..556)
                     /gene="hemG"
                     /locus_tag="OQN13_002167"
     CDS             complement(11..556)
                     /gene="hemG"
                     /locus_tag="OQN13_002167"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCS1615522.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(568..2019)
                     /gene="trkH"
                     /locus_tag="OQN13_002168"
     CDS             complement(568..2019)
                     /gene="trkH"
                     /locus_tag="OQN13_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCS1615523.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2058..2672)
                     /locus_tag="OQN13_002169"
     CDS             complement(2058..2672)
                     /locus_tag="OQN13_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCS1615524.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITSVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2672..4003)
                     /gene="pepQ"
                     /locus_tag="OQN13_002170"
     CDS             complement(2672..4003)
                     /gene="pepQ"
                     /locus_tag="OQN13_002170"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCS1615525.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4193..6382
                     /gene="fadB"
                     /locus_tag="OQN13_002171"
     CDS             4193..6382
                     /gene="fadB"
                     /locus_tag="OQN13_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCS1615526.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6392..7555
                     /gene="fadA"
                     /locus_tag="OQN13_002172"
     CDS             6392..7555
                     /gene="fadA"
                     /locus_tag="OQN13_002172"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCS1615527.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQAGLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(7745..8446)
                     /gene="fre"
                     /locus_tag="OQN13_002173"
     CDS             complement(7745..8446)
                     /gene="fre"
                     /locus_tag="OQN13_002173"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCS1615528.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8493..9986)
                     /gene="ubiD"
                     /locus_tag="OQN13_002174"
     CDS             complement(8493..9986)
                     /gene="ubiD"
                     /locus_tag="OQN13_002174"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCS1615529.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10153..10641
                     /gene="rfaH"
                     /locus_tag="OQN13_002175"
     CDS             10153..10641
                     /gene="rfaH"
                     /locus_tag="OQN13_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCS1615530.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10638..11420)
                     /gene="tatD"
                     /locus_tag="OQN13_002176"
     CDS             complement(10638..11420)
                     /gene="tatD"
                     /locus_tag="OQN13_002176"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCS1615531.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(11462..12238)
                     /gene="tatC"
                     /locus_tag="OQN13_002177"
     CDS             complement(11462..12238)
                     /gene="tatC"
                     /locus_tag="OQN13_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCS1615532.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12241..12756)
                     /gene="tatB"
                     /locus_tag="OQN13_002178"
     CDS             complement(12241..12756)
                     /gene="tatB"
                     /locus_tag="OQN13_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCS1615533.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(12760..13029)
                     /gene="tatA"
                     /locus_tag="OQN13_002179"
     CDS             complement(12760..13029)
                     /gene="tatA"
                     /locus_tag="OQN13_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCS1615534.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13108..14748)
                     /gene="ubiB"
                     /locus_tag="OQN13_002180"
     CDS             complement(13108..14748)
                     /gene="ubiB"
                     /locus_tag="OQN13_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCS1615535.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(14745..15350)
                     /gene="ubiJ"
                     /locus_tag="OQN13_002181"
     CDS             complement(14745..15350)
                     /gene="ubiJ"
                     /locus_tag="OQN13_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCS1615536.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15364..16119)
                     /gene="ubiE"
                     /locus_tag="OQN13_002182"
     CDS             complement(15364..16119)
                     /gene="ubiE"
                     /locus_tag="OQN13_002182"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCS1615537.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16214..17641)
                     /gene="rmuC"
                     /locus_tag="OQN13_002183"
     CDS             complement(16214..17641)
                     /gene="rmuC"
                     /locus_tag="OQN13_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCS1615538.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(17782..18543)
                     /gene="udp"
                     /locus_tag="OQN13_002184"
     CDS             complement(17782..18543)
                     /gene="udp"
                     /locus_tag="OQN13_002184"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCS1615539.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            18805..19617
                     /locus_tag="OQN13_002185"
     CDS             18805..19617
                     /locus_tag="OQN13_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS1615540.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            20011..21072
                     /gene="hcp"
                     /locus_tag="OQN13_002186"
                     /pseudo
     CDS             20011..21072
                     /gene="hcp"
                     /locus_tag="OQN13_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5197 a         5667 c         5301 g         4970 t
ORIGIN      
        1 ttctttatgc ttatttcagc gtcggtttgt tggttaactg ggcgatttct cgggcgaaat
       61 tcgccacctg ctcccaatcg gtatagacaa cttctttgcg cgtatccgtt tcaccgcctg
      121 acatcttcat aattagcttg atcataaaac gatcgtacca gcgataacgt gggtaacgca
      181 gcgccccggc aatgaccgcg cagcgatcgg gacgccattg cgagttcatc agaaacttcc
      241 gcgcgtagct gttggtctgt ggagtacgct tctccggttt gcgcgccacc aggtttacgg
      301 aataaaaggc gctcggcatc gaattcagtc gcgtcgcatg ttttttgaca aattcctgga
      361 acgcagagtg gtagtgacca tagcgaatag aagcaccaat gaccacacgg tcataatttt
      421 cccattgtgg ttcttcaatg cggtgcacat tggcgacatc cgtctggatc cccagttctt
      481 tcagttccga agccaggtag gaggcaatct cgcgcgtttg tccgtccctt gttgagaaaa
      541 gaattaatgt tttcacgtat tactccatca ttcacgccag aaagtcgggg taaagagcac
      601 cagcaatgta aagacctcga gacgaccaaa cagcatgttg gcaatcagga tccatttagc
      661 caccgggttc atactggtaa agttatcagc aaccacgcca agccctggcc ccaggttatt
      721 caatgtcgca acaactgaag caaaggcaga aaagtcatcc acgcccgtgg cgataatcgc
      781 cagcatactg acaataaaca ccaatgcata ggcggagaaa aatccccaaa cggcttcgag
      841 gatacgttcc ggcagtgcgc gattccccag cttaatgcta tagacggcgt tcggatgcac
      901 cagtcgtttc agctcacggt tcccctgctt aaacagcagc aggatgcgga tcactttcag
      961 gccaccgccc gttgacccgg cgcaaccgcc gataaaagct gaacataaaa gcagtaccgg
     1021 caaaaagagc ggccagcggg caatgctgtc agtcgtaaac ccggcggttg tcgccatcga
     1081 taccacctgg aaaaacgcct ggttaattgt catcagcgcc gaactgtaga cattatgaaa
     1141 ccacagtacg agtgtacaaa taaccaccag cgtaaactgt acgccgataa acatgcgaaa
     1201 ttccggatcg cgccaataaa ccttcagact acgcccactt aacagtgaaa agtgcagacc
     1261 gtagttacag ccggagatca gcaggaagat agcaatgata gtgttaatag tcgggctgtc
     1321 gaaataaccg atactggcat catgtgtcga gaagccgcca atagcgatag tcgcaaagct
     1381 atggccgatg gcatcaaagg catccattcc ggcaaaccac aacgccagcg cacaggcgac
     1441 ggtcagcaag acataaatca accacagggt tttcgccgtt tccgcaattc gcgggcgcat
     1501 tttgttatct ttcagcgggc cgggcatttc tgcgcgatag agctgcatcc cacccacgcc
     1561 gaggataggc agtatcgcaa ccgctaacac gatgatcccc atcccgccaa accattgcag
     1621 catctggcga taaaagagga tggcatgagg gagcgaatcc agccccacca gcgtagtggc
     1681 cccggtggtg gtcaggccag agaaagattc aaaaaacgca tcggtaatcg tgaggttcgg
     1741 gctttccgag aagataaaag ggagcgcacc gacactaccc agcacggtcc agaacagcac
     1801 cactatcaga aacccctcac gggatttcag ttcgcccttc tctttacggt tcggccacca
     1861 cagcatagaa ccaatggcga gggcgacaaa aaaggtctgg gtaaaagcgc ggcccgctcc
     1921 atcccggtag atgagtgcta ccagcccagg gataatcatg gtccctgaaa ataagatgac
     1981 cagtagtcca acgattcggg taatggcgcg aaaatgcatc tctgccgctt ccttagttct
     2041 gcaaaacgaa gtggggatta ttcttcaatc gctaacaatt gcaatgaacc acggctaaaa
     2101 tccgccagct ttgcggaaaa ttcagccact ttcgccgccg gaagcgccac ccgcagcaga
     2161 acgaatgcct gataatcact gttgataatt ttgccgtcac actgccccag caacgcttca
     2221 atgccggtta actgatgata ttcacattgc aaagtatatt cggttaatgg cgtcttgcgt
     2281 tgggtcgtta gctggcgcag cgcctgattc acgccgccgc catacgcttt cactaaccca
     2341 ccggtgccaa gcaatatgcc gccgtagtag cgcactacca cagaggtaat ttccccgacg
     2401 ccgctgccca ttagctgggc gagcatcggt ttacctgccg ttcctgccgg ctccccgtcg
     2461 tcagagaaac ccagctgttg agaatcatcc ggcgcacccg ccacccacgc cacgcaatgg
     2521 tggcgggcat cggggtgttc tgcccgcacc gattcaacaa acgctttcgc cgcctcaacg
     2581 ccatcggtat gcgccaacat cgttatgaag cggctttttt tgatctcttc aacgaccgtg
     2641 accggtgccg caggaattaa ccagctttcc atcacgccag tttcagatcc cgggtcatgt
     2701 tttccacgtt gttttcgtgg atcaccacgt tgtcttcgat acgaatgccg ccgaacggtt
     2761 tcagtgcttc aattttctgc cagttgaagt gcttgctgaa ctgcccttca cgccacggtg
     2821 ccagtagcga ttcaatgaag tagatacccg gttcgatggt taacaccata cccggctgga
     2881 gaatacgggt gcagcgcagg tacggatatt ttgccggtgc cgcgaggtgt gtaccgctat
     2941 catcctgcat aaaaccggct acgtcatgca cctgcaggcc cagcggatga ccgataccat
     3001 gcggcataaa cggtccggtg agatcgtttt cgaccatcgc ttcttcactc atatcggtga
     3061 tgatttgatg tttacgcagc agtttggcga tgcgctgatg gaactggatg tggtaatcca
     3121 catagctaac gcctgctttc atggtggcga tcagcgccag ttgttcatca tttacgtctt
     3181 tcaccagctg tgcgtagtcg ttgtcgcttt ttgccgacca ggtacgagtc aggtcagccg
     3241 catagccgtt atattcggcc ccggcatcca gcaggaagct gcgcatctct tccggtgcct
     3301 gatgatccag tttggtgtaa tgcagcaccg aagcgtgttc gttaagcgcc acaatgttgc
     3361 tgtaaggtac gtcggtatca cgatgaccgg tcgcggtcag ataggcgatg ttgatatcga
     3421 actcgctcat gccagaacgg aacgcttctt ctgccgcacg atgaccgttg accgccattt
     3481 tctgcgcttc acgcatacag gccagttcgt actcggtttt gaaggagcgg tagtaatgca
     3541 ggtagtcgat cacccctttc gggttgatgt tgctggcctc aatacccagt tgcagcgcac
     3601 gttccggcac cggaccgata taaccgatat tgccgcgcgc agcgggcagc aggctaccaa
     3661 tgccatcggc tttcggcagt gcgatcactt ccacatcttc agtccagaag gaggtcggca
     3721 gcggttcgac gttgtgccag taatcaaccg gcagatagaa ccacagtttc ggcttgttca
     3781 cgccatccac cagcaaccag cagtttggca cctgagttac cggcacccac gctttgaatt
     3841 gcgggttcac tttaaacgga tagggatgat cgtcgagaaa aacgttgaac agctcgccgg
     3901 agtgaataag taatgcatcc agcttgaagc gcgccagcgc atcgcgagtc cgttcttgta
     3961 aggtagctat atgattttta tagagcgagg ccagtgattc cattttttac ccttctgttt
     4021 ttttgacctt aagtctccgc atcttagcac atcgttcatc cagagcgtga tttctgccga
     4081 gcgtgatcag atcggcattt ctttaatctt ttatttgcat atttttaaca caaaatacac
     4141 acttcgatgc atctggtacg accagatcac cttgcggatt caggagactg acatgcttta
     4201 caaaggcgac accctgtacc ttgactggct ggaagatggc attgccgaac tggtatttga
     4261 tgccccaggt tcagttaata aactcgacac tgcgaccgtc gccagcctcg gcgaggccat
     4321 cggcgtgctg gaacagcaat cagatctaaa agggctgctg ctgcgttcga acaaagcagc
     4381 ctttatcgtc ggtgctgata tcaccgaatt tttgtccctg ttcctcgttc ctgaagaaca
     4441 gttaagtcag tggctgcact ttgccaatag cgtgtttaat cgcctggaag atctgccggt
     4501 gccgaccatt gctgccgtca acggctatgc gctgggtggt ggctgcgaat gcgtgctggc
     4561 gaccgattat cgtctggcga cgccggatct gcgcatcggt ctgccggaaa ccaaactggg
     4621 catcatgccg ggctttggcg gttctgtacg tatgccacgt atgctgggcg ctgacagtgc
     4681 gctggaaatc attgccgccg gtaaagatgt cggcgcggat caggcgctga aaatcggtct
     4741 ggtggatggg gtagtcaaag cagaaaaact ggttgaaggc gcaaaggcgg ttttacgcca
     4801 ggccattaac ggcgacctcg actggaaagc gaaacgccag ccgaagctgg aaccgctaaa
     4861 actgagcaag attgaagcca ccatgagctt caccatcgct aaaggaatgg tcgcgcaaac
     4921 ggcggggaaa cattatccgg cccccatcac cgcagtaaaa accattgaag ctgcggcccg
     4981 ttttggtcgt gaagaagcct taaatctgga aaacaaaagt tttgtcccgc tggcgcatac
     5041 caacgaagct cgcgcactgg tcggcatttt ccttaacgat caatatgtaa aaggcaaagc
     5101 gaagaaactc accaaagacg ttgaaacccc gaaacaggct gctgtgctgg gcgcaggcat
     5161 catgggtggc ggtatcgctt accagtccgc gtggaaaggc gtgccggttg tcatgaaaga
     5221 tatcaacgac aagtcgttaa ccctcggcat gaccgaagcc gcgaaactgc tgaacaagca
     5281 gcttgagcgc ggcaagatcg atggtctgaa actggctggc gtgatctcca cgatccaccc
     5341 aacgctcgac tacgccggat ttgaccgcgt ggatattgtg gtagaagcgg ttgttgaaaa
     5401 cccgaaagtg aaaaaagccg tactggcaga aaccgaacaa aaagtacgcc aggataccgt
     5461 gctggcgtct aacacttcaa ccattcctat cagcgaactg gccaacgcgc tggaacgccc
     5521 ggaaaacttc tgcgggatgc acttctttaa cccggtccac cgaatgccgt tggtagaaat
     5581 tattcgcggc gagaaaagct ccgacgaaac catcgcgaaa gttgtcgcct gggcgagcaa
     5641 gatgggcaag acgccgattg tggttaacga ctgccccggc ttctttgtta accgcgtgct
     5701 gttcccgtat ttcgccggtt tcagccagct gctgcgcgac ggcgcggatt tccgcaagat
     5761 cgacaaagtg atggaaaaac agtttggctg gccgatgggc ccggcatatc tgctggacgt
     5821 tgtgggcatt gataccgcgc atcacgctca ggctgtcatg gcagcaggct tcccgcagcg
     5881 gatgcaaaaa gattaccgcg atgccatcga cgcgctgttt gatgccaacc gctttggtca
     5941 gaagaacggc ctcggtttct ggcgttataa agaagacagc aaaggtaagc cgaagaaaga
     6001 agaagacgcc gccgttgaag acctgctggc agaagtgagc cagccgaagc gcgatttcag
     6061 cgaagaagag attatcgccc gcatgatgat cccgatggtc aacgaagtgg tgcgctgtct
     6121 ggaagagggc attatcgcca ctccggcgga agcggatatg gcgctggtct acggcctggg
     6181 cttccctccg ttccacggcg gcgcgttccg ctggctggac accctcggta gcgcaaaata
     6241 cctcgatatg gcacagcaat atcagcacct cggcccgctg tatgaagtgc cggaaggtct
     6301 gcgtaataaa gcgcgtcata acgaaccgta ctatcctccg gttgagccag cccgtccggt
     6361 tggcgacctg aaaacggctt aaggagtcac aatggaacag gttgtcattg tcgatgcaat
     6421 tcgcaccccg atgggccgtt cgaagggcgg tgcttttcgt aacgtgcgtg cagaagatct
     6481 ctccgctcat ttaatgcgta gcctgctggc gcgtaacccg gcgctggaag cggcggccct
     6541 cgacgatatt tactggggtt gtgtgcagca gacgctggag cagggtttta atatcgcccg
     6601 taacgcggcg ctgctggcag aagtgccgca ctctgtcccg gcggttaccg tcaatcgctt
     6661 gtgtggttca tccatgcagg cactgcatga cgcagcacga atgatcatga ctggcgatgc
     6721 gcaggcaggt ctggttggcg gcgtggagca tatgggccat gtgccgatga gtcacggcgt
     6781 cgattttcac cccggcctga gccgcaatgt cgccaaagcg gcgggcatga tgggcttaac
     6841 ggcagaaatg ctggcgcgta tgcacggtat cagccgtgaa atgcaggatg cctttgccgc
     6901 gcggtcacac gcccgcgcct gggccgccac gcagtcgggc gcatttaaaa atgaaatcat
     6961 cccgaccggt ggtcacgatg ccgacggcgt cctgaagcag tttaattacg acgaagtgat
     7021 tcgcccggaa accaccgtgg aagccctcgc cacgctgcgt ccggcgtttg atccagtaag
     7081 cggtacggta acggcgggta catcttctgc actttctgat ggtgccgcgg ccatgctggt
     7141 gatgagtgaa agccgcgccc gtgaattagg tcttaagccg cgcgcccgtg tgcgttcgat
     7201 ggcggtcgtt ggttgtgatc cgtcgattat gggttacggc ccggttcctg cctcgaagct
     7261 ggcgctgaaa aaagcggggc tttctgccag cgatatcggc gtgtttgaga tgaacgaagc
     7321 ctttgccgcg cagatcctgc cgtgcattaa agatctcggg ctaatggagc agattgacga
     7381 gaagatcaac ctcaatggtg gtgcgatcgc gctgggtcat ccgctgggtt gttccggtgc
     7441 gcgtatcagc accacgttgc tgaatctgat ggaacacaaa gacgttcagt ttggtctggc
     7501 gacgatgtgt atcggtctgg gtcagggtat tgcgacggtg tttgagcggg tttaaccact
     7561 tgccggatgc ggcgtgaacg ccttatccgg tctaccgatc ctgcaccaat gtaggcctga
     7621 taagacgcgc cagcgtcgca tcaggcatcg tgcaccgatt gccggatgcg acatagatct
     7681 aaatgccact gaataaagtt ttagttgccg ttcttcccgc ctgtcagggg cgggttttta
     7741 tatctcagat aaatgcaaac gcatcgccaa acagacgatc ttcccgcgca ttacgctcac
     7801 tgcaaaacag gtcacgagca attttcgcca tctcaaaacg tccggcaata tagatatcat
     7861 gctctgccag cgtaccatga tcctgcaaga ccgccgttaa cacggtgcca gtacgcccac
     7921 gccagcccgc ttccggttgt tcaaccaccg gcaccacttg cagaccagga tgcttcagcg
     7981 aaagcgcctc aagctcgcag agatcataca gatgttgctc ttcacgccca ccccagtaaa
     8041 tggtgatatc gcgattggga ttacgcgcca acgccgtcaa taaaatcgag cgcgcatagg
     8101 agaacccggt gccgcccgca atcaaaatca tcggacgctc ttcatcatcg cgcagccacg
     8161 cttctccgtg gggaatatcg accacgattt gatgatcttt gaggatgcgg tccatgactg
     8221 ctttcgcgta aaggttgatt tcagaagcgc caatatgcag ctcgataaac cctttttcat
     8281 ccggcgtcga agccattgaa aacggacgtt tgtcgcgctc atccatcact accatcaaat
     8341 actgaccagc acgaaaagaa aaggccgcgt ctggtacgat tcggacacgg tatacagtat
     8401 ccgtgatagc ttctaccgag gtcactttac agcttaaggt tgtcatgcgc tttctctgtc
     8461 ggatcaaata aatagggcaa aactaacgcg catcaggcgc ttttaccgtt gttaaaaata
     8521 gccagttcat cccagatggc gtcaatatgc gcgacgacat caggatcttt tttgatggga
     8581 cgtccccatt cacgctgggt ttcccccggc catttattcg tggcatccag ccccattttt
     8641 gaacccagcc cggagacagg cgaggcaaaa tccagataat caataggcgt attttctacc
     8701 agaacagtat cccgcgccgg gtccatacgg gtggtaatcg cccaaatcac atcgttccag
     8761 tcgcgtgcgt tgacgtcatc atcgcaaacg atcacaaatt tagtgtacat aaactggcgt
     8821 aagaacgacc agacgcccat catgacgcgc ttcgcgtgtc cggcgtactg ttttttgatt
     8881 gtcactaccg ccagacgata agaacagcct tccggtggca ggtaaaaatc gacaatttcc
     8941 gggaactgtt tttgcagaat cggcacaaac acttcgttca gtgcgacacc cagcaccgcg
     9001 ggttcatccg gcggacgacc agtataggtg gagtggtaaa tcgcatcttc acgctgggta
     9061 atatgcgtca cggtaaatac cgggaaacta tcgacttcgt tatagtaacc ggtgtggtcg
     9121 ccatacggcc cttccggcgc agtttcgcct tgttcgatat acccttccag cacaatctcc
     9181 gcactggcgg gcacttcaag gtcattggag atacacttca ccacttcggt cttggtaccg
     9241 cgcagcaatc cggcaaacgc atactctgaa agtgtatccg gaacgggggt gactgcaccg
     9301 agaatcgtgg cgggatcggc acccagcgcc acagaaaccg ggaaacgttc gcccggatgc
     9361 gccgcacacc actcctgata atccagcgcg ccgccgcgat gcgacagcca gcgcataatc
     9421 agtttgtttt taccaatcag ctgctggcga taaatgccca gattctgccg ctctttatgc
     9481 gggccgcgcg ttacggtcag cccccaggta atcagcggcg cggcatcttc cggccagcag
     9541 gtcataatgg gaatgcgatt gagatcgacg tcatcgccag agacgatttt ttgttggcag
     9601 ggcgcaccac gcagccgctt tgtcggcatg ttcaatactt gcttaaactg cggcagttta
     9661 tcaaacaggt cgcggaaacc ttttggcggc tcgggctctt tcagaaacgc caataattta
     9721 ccaacttcac gcagcgccga aacatcttcc tgccccatgc ccatcgccac gcgctttggc
     9781 gtaccgaaca ggttgcacag caccggcatt gagtagcctt tagggttttc gaacaacagc
     9841 gcaggcccac cggcacgcaa agtgcggtca gcaatttcag tgatttccag atgcggatcc
     9901 accgggagcg tgatacgttt tagctcaccc tgctgttcaa gcagcgtcaa gaagtcgcgt
     9961 aaatcgttat atttcatggc gtccattgta gcctcttaat ctacgcccat tatacggcgt
    10021 tcatctttgc gatgctgtaa atttgttaaa ttagcgtgaa ctctgacggt ataacgcaaa
    10081 ccggggaata taattaactt agcgtaaagc ttttgctatc cttgcgcccc gattaaacgg
    10141 ataagagtca ttatgcaatc ttggtattta ctgtactgca agcgcgggca acttcaacgt
    10201 gcccaggaac accttgagag acaggctgtg aattgcctgg caccgatgat caccctggaa
    10261 aaaatcgtgc gtggaaaacg tactgcagtc agtgagccat tgtttcccaa ctacctgttt
    10321 gtcgaatttg atccagaagt gattcatacc acgactatca acgcgacccg cggtgtcagc
    10381 cacttcgtgc gctttggcgc gtcgctagcg atagtcccat cggcggtgat tcatcagcta
    10441 tcggtatata aaccgaaaga cattgtcgat ccggcaaccc cttatccggg agataaggtg
    10501 attattaccg aaggcgcgtt cgaaggcttt caggccattt tcaccgaacc cgatggtgag
    10561 gctcgctcca tgctattgct taatcttatt aataaagaga ttaagcacag tgtgaagaac
    10621 accgagttcc gcaaactcta aaacgcaatc ccaaaccgtg ttttgacatt agcatccgtg
    10681 gtggcagcca gccatgcggc atcttctcca cgccagtgcg caatacgttg caaaatatgg
    10741 ggcagatggg ctggctcgtt gcgccgggat gatggctttg gcgtgagatc gcgagggagc
    10801 agatacggcg catcagtttc gatcagtaat ttttccgccg gaatcaacgg caaaagttcc
    10861 cgcagctcca gtccgcgtcg ttcatcgtaa acccaaccgg taatgccgat ataaattcca
    10921 tgcgccacgc acgcctgcat ctcttcgcgt gtgccggtaa agcaatgaag aaccgcacca
    10981 ggcagtttat ccagccacgg ctccagcaat gtcataaacc gctcgtgggc atcgcgacag
    11041 tgcataaata ccggcatgtt taaatctgcg gcaatgcgta gctgggcaac aaaagcgcgt
    11101 tcctgctctt ccggcgtcga aaagttgcgg ttaaagtcga gaccacattc accaatcgcc
    11161 accacttctg gctgcgcggc cagctcaata atcgcttctt cagtcgcagc ttgccactgg
    11221 ctgctgtcgt gaggatgtac gcccgccgtt gaccaacagg acgaatactg acgcgccagc
    11281 ttttgcgcct gctggctttc acgcaggtta gtgccggtga tgagtagccc attaactccc
    11341 gcgtcaaaag cgcgcgctac aacatcatca cggtctttcg caaattgcga actggtcaaa
    11401 ttaacgccga tatcaaacat cctgtactcc atatgacaac cgccctgacg ggcggttgaa
    11461 tttattcttc agttttttcg ctttctgctt cagcgtcgtt ttcctcttcc cgatttcgcc
    11521 ctttaccaac gtaaaagcgt gagaagaaga caccgatttc aaacagacag tacatcggga
    11581 tcgccaatag cgtttgcgag aagacatccg gcggcgtcag caacatcccg acaacgaatg
    11641 caccaaccag cacatacggg cgttttttgc gtaagtcttc tggcgaggta atccccatcc
    11701 agcacagcag cacaattgcc accggcactt caaaggagac accaaacgcc ataaacagcg
    11761 ccataacgaa gcttaaatag ctggcgatgt cggtggatac ctgcacccct tccggcgcgg
    11821 tattggcaag gaagccaaat gccagcggaa agaccacaaa gtaggcgaat gccatgccga
    11881 tataaaacag cagagagctg gaaaccagca gcggcaccac caggcgacgt tcatgcttat
    11941 acagcgctgg ggcgataaat gcccacacct gatagagaat caccggcgct gacagaatca
    12001 gcgacaccat aaaggtcagc ttgatcggcg taaagaacgg cgaggccacg tcggtggcga
    12061 tcatcgttga accttgcggc aactgcttga tcaatggcgc ggataccagg tgatagatgt
    12121 cattggcgaa atagaccaga cacaggaata tcacgatcac cgcgataatg cagttcagca
    12181 gacgcttacg cagctcaatt agatgcgtga taagcggttg agtatcttct acagacatgt
    12241 ttacggttta tcactcgacg aaggggaagg tgcagcggtt ttcggttcag cgtccgcagc
    12301 aggttttacc accggctctg gcgtggtttc tggcttctgt tccggcgaac tggcctgcgt
    12361 ttgtgcagcg gcaggcgtta cgccctcatg cgcagcttca ttatctttca ccaccgggtt
    12421 atggatggtg tgcgcttcat cgctcgcctt ttcaggatcg tttgcaacgt aggaacgctt
    12481 catcgactcc gcggcctggc gtagttcatc catcgacgct ttcagttcgg gcgtcaggtt
    12541 agtgaggctc gccttttcaa cctttttcag actgtcctga aactcctgga gttttaactc
    12601 ctgggtcagt tcgttctgca ccgttgtcgc cagtgaacgc aacgcgcgaa tccagcccgc
    12661 taccgttttt accgccacag gcagtcgttg cggccccaga acgacgaggc cgatgatgaa
    12721 caccaatagc agttcgctaa aaccgatatc aaacacggat tacacctgct ctttatcgtg
    12781 gcgcttcgcg tcttctgttt tagcctgttc ctgattcgta tccgcctgct tatcggcgat
    12841 agttttcgca gtaaaatcag catcctgact ggttttatcc tgctttggtt catcatcgct
    12901 cattgctttt ttaaagcctt tgatcgacgc accaagatcg gaaccgatgg agccgagctt
    12961 tttggtgcca aaaagcagta caacgatgac ggcaataatc aataactgcc aaatactgat
    13021 accacccata catgttcctc tgtggtagat gatgattaaa caaggccgca ttatacgtta
    13081 cacgacccgc cttgagcgat gaaaaaatca gcgtgttttg cgccaaccga caaaccaggc
    13141 gatcagacca cctgccatta accagccggg catcagcccc cattcaggtc ggctgaccaa
    13201 caagaatgtg ccacttaata ctaacgtagc gccaattccg agaaaataac gcgattgtcc
    13261 ctgacgtaca tgatttgact gaagctcgcg ggcaatctta tcaacactgt gctgtaaata
    13321 cttgccctgg cgcaaactgt cgtaaaccaa ttcaggcagt tctggcattt tttcgaccca
    13381 gaacggcgct ttttctttaa atgctctcac cagggcagga ataccgacct gatctttaat
    13441 ccacgactcc aggaaaggct tcgctgtttt ccataaatcg agctgcggat aaagctggcg
    13501 tcctacccct tcgacataga gcagggtttt ctggagtaac accagttgcg gctgcacttc
    13561 catattgaag cgacgcgccg tattaaacag atttaacagt acatgtccaa acgaaatttc
    13621 ggccagcggt ttctcaaaga taggttcaca gaccgtacga atggcaaatt cgaactcttc
    13681 aacgttggta tctggtggca cccagccaga atcgacgtgt agctctgcca ctttgcgata
    13741 gtcgcgatta aagaaggcga taaagttttc tgccagatag cgtttatctt ctttgtttag
    13801 cgagccaaca atcccgcaat caatgccgat atatttcggg ttttccgggt gttcatagct
    13861 tacgaaaatg ttgccagggt gcatatcggc atggaaaaag ctgtcgcgaa agacctgagt
    13921 gaagaacacc tgcacgccac gttccgccag caatttcatg ttggtgccat ttttctccag
    13981 cgtcgcaaca tcagacaccg gaatgccgta aatgcgctcc atcaccatca tcccttcact
    14041 acaatagtca gggtaaactt ccgggatgta gagcatcggg ctgtcttcaa aatttcgccg
    14101 aagctgaatg gcgttggcag attcccgcag caaattcagt tcatcaatca aggtcttttc
    14161 gtactcgcgc accacttcgg ttgggcgcag acggcgacca tccggcagca aacgcggcac
    14221 ccagcgagcc agacggtaga taagtttcag atccgcttta ataaccggca aaatatccgg
    14281 gcggatgact ttaatcacca cctctttacc attcgatttc aatcgcgcgg tatgaacctg
    14341 ggcgatagaa gcagaagcca gtggcttgat ttcaaaatcg tcaaaccacg cttctaccgg
    14401 caagccgccc attgcagctt caatctgctg cttcgccagc ttgccatcaa acggagcaac
    14461 tttgtcctgc aataacgcca gctgatcggc aatatgcggt ggaaaaagat cgcggcgggt
    14521 tgataacatt tgcccgaact tgatccaaac cggccccagt tcttgcaggg ccagtcgtag
    14581 tcgctcacct aaaagtttgt ctttatgccg atttggcatc cagaataatg agtatcgcca
    14641 tagccgtagc ggcagggtga tacgcatttt ggggatcagt tcatcaagtc cgtagcttaa
    14701 aaaagtgcga atgatgaaat ataggcgccg tacttcacct ggcgtcattt agcctccagt
    14761 ttttccagcc gtttggtcag ggcatcaaca gcacgctcga cggcagccgt ctcttccgca
    14821 aaccaggcca cttcaagcgg accgggtgcc atacgccact cttcagtaat ggcttccgcc
    14881 acataacgtt gctggcgctt aatgccgtga tgcaggaact ttgcgcctcc gcgcatggct
    14941 ttgctgattc cttcagcggc gatatcaccg gtataagggg ccagcagttc cgcagggtcg
    15001 aactctgcca gatctgccag cgcaacgaag ttttgcacca cctgaatatc gccctgcact
    15061 tccagctcac cactgcgaat cagtgcggta agctgctggc gatcgcgaag tttcggcaac
    15121 acactggcgt aggcgataac ggtgcagtca gcatcgcctg cccattcgcc cagtacatca
    15181 acctggcgtt cgctgaacac cagaatcaat gacgtcgaaa agccttttac ctccacgcgc
    15241 aatactttac ccagcagacg cgagcgggct gttttcagcg cgggtgagcg atacaggaag
    15301 gtgttgagca gactttcaat tcctgccgtc actaaaggtt taaaaggcat ttccggtctc
    15361 ctgtcagaac ttataaccac gatgcagcgc cacaacccct gccgtcagat tgtagtagtc
    15421 gacactttcg aatccggcat cctgcatcat ggctttcagg gtatcctgat cgggatgcat
    15481 acggatggat tctgccagat aacggtagct gtcggcgtcg ttcgcgacca gtgagccaat
    15541 acgcggcagc acatggaagg agtatgcatc ataggctttg ctcagcggct cgataattgg
    15601 cttcgagaac tcaagcacca gcaggcggcc gccgggtttc agcacgcgat acattgaacg
    15661 cagtgcttta tctttgtcgg tgacgttacg cagaccaaac gaaatggtga tgcaatcaaa
    15721 ggtgttatcc gggaacggca gcgcctcagc gttcgcctga acatactcaa cgttgccaat
    15781 cacaccgata ttacgcagct tctcgcgtcc cattttgagc atggattcat tgatatcagc
    15841 aaggaccact ttgccagttt ctccgaccag gcgggagaat ttcgctgtca ggtcgccggt
    15901 gccaccagcc agatccagca cggtctgccc acggcgtacg ccgctgcaat caatcgtgaa
    15961 tcgcttccac aaacgatgaa taccaaatga catcaaatca ttcatgacat cgtattttga
    16021 tgccacggaa tggaaaacgt gggcgaccat atccgctttt tgttccttcg cgacggtctg
    16081 aaaaccaaag tgcgtcgttt cttgtgactt atccaccatc tcaatgcctg ctcatcaaaa
    16141 aattgttcca gaagtgtaac agattgggcg tcgatgccct agatttctac ccggcttaac
    16201 tacccctaat gggctagcgc gactgctgat tatattcatc atcgcgttga taagcttcat
    16261 cattcggctg ctccggaacc gaccgaagtc gatactcttc attctggctc accgcctgtt
    16321 cagccaaatc cggattaatc tcgcgtttaa tttctactcc taaaccgcga aacgcttctg
    16381 cctgcgccag cacatttccg cgacctgaag agagtttttt cattgcctgg cggtagttat
    16441 cctgcgcttt gtcgagactt tgaccaatcg cggacatgtc atcgatgaac aaacgcatct
    16501 tgtcgtacag cttgctggca cgatcggcga tttgctgggc gttgcggctt tgatgctcat
    16561 aacgccacag gttggcaata gtacgcaacg ccaccagcag cgtagtcggg ctaaccagca
    16621 tgatattgtt tttcaacgct tcggtgatca gctccggctg gcggtcaagc gccagtaaaa
    16681 aagcgggttc aacgggaata aacatcagca cgtaatccag agttcgcagc cccggcagct
    16741 gttgataatc tttgcgtccc agcaaacgga tatggttacg caccgacgcg atatgttcct
    16801 gtagcgcgct ttcgcgggtg tagtcgtctt cggcgttaaa atagcgttca taggcgacca
    16861 gcgtcatttt ggcgtcgatc accacatctt ttccctgcgg cagccgcacg atgacatccg
    16921 gctgcatccg cgagcgggcg tcattttcga tgctgacctg ggtttcatat tcataccctt
    16981 cacgcagacc ggaagcctcc agcacccgcg tcaatactac ctcgccccag ttgccctggg
    17041 ttttattgtc gcctttcagc gcgcgcgtca ggttgatcgc ttcctgggcc atttgcgcgt
    17101 tgagttgctg gagattgcga atttcgtggg tcagggtatg gcgttcttgt gcttctttac
    17161 cgaagctgtc ctgaacctga cggcggaaac cgtccagttg ttcacgtagc ggcgacaaca
    17221 ggctgttcag actctgacgg ttttgctcat caacccggcg attgctgtgc tcaaaaatac
    17281 ggttggcgag gttttcaaac tgctcactga ggcgctgctc gctgttaatc atctggcgaa
    17341 ttttatcgtc agcatgttgc tgtgcggctt ccatccgcgt ggttacttca cgcagatcgg
    17401 cctccagaga ggtgttaata ctttgcaggc tgcgcacttc gttattgagt aactcgcact
    17461 ctgcacgcca gtgctcgctt tgggtaattt gttgttttgc cgcgcttaac tccgcgacca
    17521 tctcttcacg ttcagctaat tgctcggctt tttgctgcgc atgttgataa ctggcaaaca
    17581 gccagccaat tgccacaccc accaacgcaa taactgcgta aaccatgatt gagaaatcca
    17641 caacgcctcc tgacgcaaca acttacccgc acaaaataga attgtgctgt acaaacgtcc
    17701 agttggaaag gcatttcctg cgaggcgcta cgcaaaaata caaaaggccg aacgcgtcgg
    17761 ccttcagaca ggagaagaga attacagcag acgacgcgcc gcttccacca cgattttcac
    17821 cgcttggctt tcagtttgtt tcatcgtctc agcattcggg atctcttgct gggtgcggtt
    17881 aacgataaca cccgctacca taccggcacg caggccctga cttgcacaca tggtcagcag
    17941 ggttgcagat tccatttcat agttcattat gcccatcgcc tgccactctt ccatagaacc
    18001 tttaaagtga cgaactacgc gaccagagta agtatcgtaa cgttcctgac ctgggtagaa
    18061 ggtatcagaa gaagctgtca cgccaacgtg agttgtcgcg ccaatggatt tcgcagcttc
    18121 aaccagcgca gtcgtacatt cgaaatcagc gacagccggg aattccagcg gtgcgaagtg
    18181 caggctcgcg ccatccagac ggacagacgc cgtggtaacc aggacatcac ccacattaat
    18241 atgcggctga atagcgcccg ttgtaccgat acgcaggaag gtgcgaatgc ccagctgtgc
    18301 cagctcttca acagcaatag aggtagacgg gccgccgata ccggtagagc agacgataac
    18361 aggtttacca tccagctctg cacgccaggt agtgaattcg cggtgagatg ccagcttaac
    18421 cggcttatcc atcagcgcgg cgatcttttc cacacgatcc gggtcgccag ggacgatggc
    18481 aagcgtagcc ccttgtaaat cgtttttagt gaggccgaga tgaaaaacat cagacttgga
    18541 catatacaac tcctctgtga atcggtttag tcagaagaag caaaaagaca ctttaccgaa
    18601 gggtttaaca ttttttcgtg atactcatca ccatgacgca aatgcgttgc ataaatttac
    18661 ccaccaaaag tgattcaaat cacataaatc actcttcatt ctgcaggact gcataaaaga
    18721 tgagccgatt caggcaatgt gatttgttgc atgctgacta tagttaacat tgcgcaacat
    18781 ttttataagg gtacggagaa taccatggca acaacacaac aatctggatt tgcacctgct
    18841 gcatcgcctc tcgcttcgac catcgttcag accctggacg acgcgattgt ggcgggcttc
    18901 acctctatcc cttcacaagg ggataacatg cctgcttacc atgccagacc aaagcaaagc
    18961 gatggcccac tgccagtggt cattgtagtg caggaaattt ttggcgtgca tgaacatatc
    19021 cgcgacattt gtcgccgtct ggcgctggag gggtatctgg ctatcgcacc tgaactttac
    19081 ttccgcgaag gcgatccgaa tgattttgcc gatatcccca cgttgcttag cggtctggta
    19141 gcaaaagtgc ctgactcgca ggtgctggcc gatctcgatc atgccgccag ttgggcttcc
    19201 cgcaacggcg gcgatgttca tcgtttaatg atcaccggat tctgctgggg tggacgtatc
    19261 acctggctgt atgccgcgca taatccacag ctaaaagctg cagtggcgtg gtacggcaaa
    19321 ctgacgggcg acaagtcgct gaattcaccg aaacaacctg ttgatatcgc aaccgatctt
    19381 aacgcgccga ttctcggctt atatggcggt caggataaca gcattccgca ggagagcgtt
    19441 gaaaccatgc gccaggcgct gcgggctgct aatgcgaaag cagagattat cgtgtacccg
    19501 gatgccgggc atgcattcaa cgctgattat cgcccgagct atcatgccga atctgcgaaa
    19561 gatggctggc agcgtatgct ggaatggttt aagcagtatg gagagaagcg gagttagttt
    19621 cctcttagtc tatatttgag atggtgagta aacgagtgcg ttttgctgcc atctctgtcc
    19681 cccaaaaaat aatcagtatg aattgattgg agattacgac tccgctattt gaccttaaga
    19741 aagtcggcaa gtcatagtca taaatgagtc tcttcaatgg aagacacctt agagatttac
    19801 catcaactcc cattcacaaa caaacattaa ttattatcaa cacattcaca ttaattataa
    19861 atcatctcag accttcctga taaaaagata ttaaagtgaa acattttgtt tttcacattt
    19921 tagttcatca cttttaggcg taaataaagg taaaaaaaac aacaatacta tttctctttc
    19981 acaaaagaat taacaaaagg tttttcaagg atgagtgata ttatttacct gaccataacc
    20041 ggagaacaac aaggatgtat ttcctctcgt tgtggaacga gcgcatcaat aggtaatcgc
    20101 tggcagattg gccatgatga tgaaatcttc gcgttctcgc tctctaatag tattacgaat
    20161 acaggcaaag ggtcataatt gcatggcctt agtttctgta aattgattga caaaagctcc
    20221 ccgttactta ttaacgctat taacaataac gagcagttgt tcatggagtt tgacttctat
    20281 cggatcaata ggtttggtcg atgggagaaa tattacaaca ttcaactccg cggcgcgctc
    20341 ttatcagcaa tcaatcacct gtttactgag aacaatctgg atacagaagc catcactgtc
    20401 agttattaat atatactctc caggcatctg attgctaata ctgaatttag ttattgggca
    20461 tttcccgata actacaaccg tctgtttata ccacggccaa aaactaaagc tggtaatggt
    20521 ttcaaaacac ttaatagtaa agcggttggc agactgcttg ctgctgtcgg catatacaat
    20581 gggaatatcg aaggattcag ggaaacagca gacaagttgg ggggagatgc acctgctggt
    20641 tatgatcaat ttatggataa caaagggtta ctcatcgctg gtgcttcaat agttgctgga
    20701 gcagttctgg ggagaatgag actccctgat ttggaatcac ttgagcattt cggagccaga
    20761 ggagcagtat caggaagacc atttaatccc gagcttgccg gtggtccaat agaaaacctg
    20821 acgacagatg gcgtaacaat caacagggaa ggtatcgcta ttgttgaaaa acatatagcc
    20881 agatttggtc atgatcctgt aaatgaagtg atgatcaata gactgaaaga cattgagaaa
    20941 ggaaaaatcc ccccagaaca agtagatctt aacttctata ctcatgagtg tcgggaatat
    21001 cagcgttact gtaatcttgg ttgggaaaca ggacaacctg atggtgacgc aggctatgca
    21061 ctctggaatc atactggatt tgcccctata tttccagaca tctgttatca cttaacccat
    21121 tacaagcccg ctgcc
//
LOCUS       DAJVRF010000064        21046 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJVRF010000064 DAJVRF010000000
VERSION     DAJVRF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.064"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            1..856
                     /gene="espL1"
                     /locus_tag="OQN13_002187"
                     /pseudo
     CDS             1..856
                     /gene="espL1"
                     /locus_tag="OQN13_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1028..1135
                     /locus_tag="OQN13_002188"
     CDS             1028..1135
                     /locus_tag="OQN13_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1615541.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1188..1946)
                     /locus_tag="OQN13_002189"
     CDS             complement(1188..1946)
                     /locus_tag="OQN13_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCS1615542.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2233..3162
                     /gene="pfkB"
                     /locus_tag="OQN13_002190"
     CDS             2233..3162
                     /gene="pfkB"
                     /locus_tag="OQN13_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCS1615543.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3263..3553
                     /gene="ghoS"
                     /locus_tag="OQN13_002191"
     CDS             3263..3553
                     /gene="ghoS"
                     /locus_tag="OQN13_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCS1615544.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3659..4519
                     /locus_tag="OQN13_002192"
     CDS             3659..4519
                     /locus_tag="OQN13_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCS1615545.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4560..5096)
                     /locus_tag="OQN13_002193"
     CDS             complement(4560..5096)
                     /locus_tag="OQN13_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCS1615546.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5243..5911
                     /gene="hxpB"
                     /locus_tag="OQN13_002194"
     CDS             5243..5911
                     /gene="hxpB"
                     /locus_tag="OQN13_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCS1615547.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLRDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6074..6664
                     /locus_tag="OQN13_002195"
     CDS             6074..6664
                     /locus_tag="OQN13_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1615548.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6797..8188
                     /gene="tcyP"
                     /locus_tag="OQN13_002196"
     CDS             6797..8188
                     /gene="tcyP"
                     /locus_tag="OQN13_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCS1615549.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(8192..9008)
                     /locus_tag="OQN13_002197"
                     /pseudo
     CDS             complement(8192..9008)
                     /locus_tag="OQN13_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9285..9548)
                     /gene="cedA"
                     /locus_tag="OQN13_002198"
     CDS             complement(9285..9548)
                     /gene="cedA"
                     /locus_tag="OQN13_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCS1615550.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            9731..11992
                     /gene="katE"
                     /locus_tag="OQN13_002199"
     CDS             9731..11992
                     /gene="katE"
                     /locus_tag="OQN13_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCS1615551.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(12250..13008)
                     /gene="chbG"
                     /locus_tag="OQN13_002200"
     CDS             complement(12250..13008)
                     /gene="chbG"
                     /locus_tag="OQN13_002200"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCS1615552.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(13021..14373)
                     /gene="celF"
                     /locus_tag="OQN13_002201"
                     /gene_synonym="chbF"
     CDS             complement(13021..14373)
                     /gene="celF"
                     /locus_tag="OQN13_002201"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCS1615553.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14478..15320)
                     /gene="chbR"
                     /locus_tag="OQN13_002202"
     CDS             complement(14478..15320)
                     /gene="chbR"
                     /locus_tag="OQN13_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCS1615554.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(15328..15678)
                     /gene="chbA"
                     /locus_tag="OQN13_002203"
     CDS             complement(15328..15678)
                     /gene="chbA"
                     /locus_tag="OQN13_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCS1615555.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(15729..17087)
                     /gene="chbC"
                     /locus_tag="OQN13_002204"
     CDS             complement(15729..17087)
                     /gene="chbC"
                     /locus_tag="OQN13_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCS1615556.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(17172..17492)
                     /gene="chbB"
                     /locus_tag="OQN13_002205"
     CDS             complement(17172..17492)
                     /gene="chbB"
                     /locus_tag="OQN13_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCS1615557.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(17791..18129)
                     /gene="osmE"
                     /locus_tag="OQN13_002206"
     CDS             complement(17791..18129)
                     /gene="osmE"
                     /locus_tag="OQN13_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCS1615558.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18331..19158
                     /gene="nadE"
                     /locus_tag="OQN13_002207"
     CDS             18331..19158
                     /gene="nadE"
                     /locus_tag="OQN13_002207"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCS1615559.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            19388..20275
                     /gene="cho"
                     /locus_tag="OQN13_002208"
     CDS             19388..20275
                     /gene="cho"
                     /locus_tag="OQN13_002208"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCS1615560.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20235..20810)
                     /gene="ves"
                     /locus_tag="OQN13_002209"
     CDS             complement(20235..20810)
                     /gene="ves"
                     /locus_tag="OQN13_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCS1615561.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         5611 a         5198 c         4823 g         5414 t
ORIGIN      
        1 acacgaatgc catttgcccc tatacatcca tcggcaatgt tgttaataat ggccacaggc
       61 cagactcaat agcttattac actattcaaa cagttgccca tactccctga aaaagaaatc
      121 attgaaataa taactgcgca gaatagcgtt ggtacacctg ctttatttct ggctatgatg
      181 aacggacaaa ctgacaacgt aaaaatattt atgcaagaaa ttcagtcact ggtatacaat
      241 catatcattc atgaagataa tctggttaaa ttactgcaaa ctaaaagtgc taacgaaaca
      301 cctggactct atatctccat gttgtatgga ttcgatgaaa taatcgatat ctttctgaat
      361 gcattaacca ctccaatagc acaagaactt ttaaacaaaa aaaatggtga tgagtatttt
      421 agccatgaaa atacatgatg gtgagccagg attatacgcc gcaatggaaa ataatcaccc
      481 tttgtgtgtc acacggttcc tctctaaaat taatggcatc gcctttaaat acaagttgag
      541 caaagctaac atcatggatt tattaaaagg agctacagca cagggaaccc ctgcattata
      601 catcgccatg agcaagggta atgaagacgt cgtgttatct tatatatcga cgctgggtgc
      661 ttttgcaaaa aaacattctt ttagtcaaca tcagttattt acactattgg ccgctaaaaa
      721 tcatgacaac atgtcagctg ttcatatagc cattcatcat aagcattata aaactgtaga
      781 aacatattat gctgctataa atgtcatcag ccaaagcctg agttttagtg ctgatgaaat
      841 aaagacgtat ttataaccag ctatttacga tatatatcga tacataattt tatttttttc
      901 ataaaacgca gaaacagtga ccatgtgcac aacgccaccc gacggctgta ataacaaaaa
      961 atcccttgat gcctatccct tttgttacac tccgttatca cgcacaagag atatgcagga
     1021 cactggtatg ccgactaaac gctttgataa aaaacactgg aagatggtgg tggtgctact
     1081 ggcaatctgt ggcgctatgt tgttgctacg ttgggcagca atgatttggg gctgagcagt
     1141 gacaaacgaa gacagccaga ccgcataaca cggtccggca gataatttta catgctgtag
     1201 cccagcgata aggttgtccg acgatcggta tgttccggtg cagactctgg aggttcagag
     1261 ttccatgtga cgttgtaagc caccttcagt ccaaaatgtt cattaatagc aacatttaat
     1321 gcgctttcgg agttcagcgt tgtatcttcc gcgccaaaga ccgaaacacc ctgcgtaaat
     1381 ttagcgttgt cagtcaactg ccatgcataa gccccggagg catagcccag cggctgggtt
     1441 tcactggcat tgtcggtata tttgtcgtaa cgcacacctg ggccgaattc aaagcggaaa
     1501 ctgtgtaccg ggccattcaa aaactggcga ccataacccg cggtcaacac atcgcgctct
     1561 cgatagccgt tataacggtc tgtcagccag cttgcctgac caaataaata gtcataatca
     1621 gttaaattaa aacggctacg cccgcccgcc gcatattttt ctgaagaacg ctcatcatta
     1681 gaagaagtat tactggcgtt cccccacagc gaccaggcag tggtttgtcc ataccaggtc
     1741 atggtggtgt cagccgtaag tgaggagctt ttcgtattgc ctgattgtgc aagatatcct
     1801 gcgttcagat taccttcgaa aggtttttta gcgctggcag gatcgtccat gacagtaaaa
     1861 acggaatcat cggcagctgc attcagtgac gcaaacatgc cccccgccaa cataacgatg
     1921 gcaggaactg tcttcaaaag cttcatttat caagagtccg tacaacaaaa aaagagacca
     1981 tcgcggtccc ggaaactttc ttaaggatca aagattagcg tccctggaaa ggtaacgaat
     2041 tataaaaagg cgcgaataac ttagcaatgt attcttattt cattttttga ataagcatgt
     2101 ggcgaaaaca gatttttatt tatatatatt tatctgcaaa attttaaata aagctccaat
     2161 aaatcatatt gttaatttct tcactttccg ctgattcggt gccagactga aatcagccta
     2221 taggaggaaa tgatggtacg tatctatacg ttgacacttg cgccctctct cgatagcgca
     2281 acaattaccc cgcaaattta tcccgaagga aaactgcgct gtaccgcacc ggtgttcgaa
     2341 cccgggggcg gcggcatcaa cgtcgcccgc gccattgccc atcttggagg cagtgccaca
     2401 gcgatcttcc cggcgggtgg cgcgaccggc gaacacctgg tttcactgtt ggcggatgaa
     2461 aatgtccccg tcgctactgt agaagccaaa gactggaccc ggcagaattt acatgtacat
     2521 gtggaagcaa gcggtgagca gtatcgtttt gttatgccag gcgcggcatt aaatgaagat
     2581 gagtttcgcc agcttgaaga gcaagttctg gaaattgaat ccggggccat cctggtcata
     2641 agcggaagcc tgccgccagg tgtgaagctg gaaaaattaa cccaactgat ttccgcttcg
     2701 caaaaacaag ggatccgctg catcgtcgac agttctggcg aagcgttaag tgcagcactg
     2761 gcaattggta acatcgagtt ggttaagcct aaccaaaaag aactcagtgc gctggtgaat
     2821 cgcgaactca cccagccgga cgatgtccgc aaagccgcgc aggaaatcgt taatagcggc
     2881 aaggccaaac gggttgtcgt ttcccttggt ccacaaggag cgctgggtgt tgatagtgaa
     2941 aactgcattc aggtggtgcc accaccggtg aaaagccaga gtaccgttgg cgctggtgac
     3001 agcatggtcg gcgcgatgac actgaaactg gcagaaaatg cctctcttga agagatggtc
     3061 cgttttggcg tagctgcggg gagtgcagcc acactcaatc agggaacacg tctgtgctcc
     3121 catgacgata cgcaaaaaat ttacgcttac ctttcccgct aacaaaaaca ttcccccagc
     3181 attgggggaa tcaccaccaa cctgtcggca acgcgtttct ccgactttgc tcaaaagtca
     3241 tgtgataaca aaggggtgaa ctatggccag tggcgatctt gtccgttatg tcataaccgt
     3301 catgttgcat gaggatacat tgactgaaat taacgagttg aataattacc tgactcgcga
     3361 cggttttttg ctcaccatga cggatgatga gggaaatatc catgagctgg ggactaacac
     3421 ttttggactt atcagtaccc aaagtgaaga agaaattaga gaactggttt cggggcttac
     3481 ccaaagtgca accggcaaag atcctgaaat caccatcacg acttgggagg aatggaatag
     3541 caacagaaaa taaatggttt ttgggcaata atcagtctgt ggtgtgcgtt agctcgtgtt
     3601 tttacaccgc attcttgcgc taaccttatg atctggcaga caacatggga gagacatcat
     3661 gtggcaggca atcagtcgtc ttttgagcga gcagttaggt gaaggcgaaa tcgaactgcg
     3721 taatgaactg cctggcggag aagtccatgc cgcatggcat ttgcgctatg caggacatga
     3781 ctttttcgtc aaatgtgatg aaagggaact gcttcccggt tttaccgccg aagccgacca
     3841 actggagtta ctgtcgcgta gtaaaaccgt caccgtgcct aaggtttggg cagtaggcgc
     3901 tgaccgtgac tacagttttc tggtgatgga ttatctccca cctcgtccgc tggatgcgca
     3961 tagcgcattt attcttggtc agcaaattgc gcgtttacat caatggagtg accaaccaca
     4021 atttggcctc gattttgata actcgctctc cacaactcca cagcccaaca cctggcaacg
     4081 tcgctggtca acgttttttg ctgaacaacg gattggctgg cagttggaac tggcagcaga
     4141 gaaagggatc gctttcggca atatcgacgc catcgtcgag catattcagc agcgtctggc
     4201 ctcgcatcaa ctgcagcctt ctctgttgca cggcgattta tggtccggca actgtgcgct
     4261 gggtccggat ggcccgtaca ttttcgaccc tgcctgctac tggggtgacc gagagtgcga
     4321 cctggcgatg ttaccgctgc atactgaaca gccgccacaa atctatgacg gctatcagtc
     4381 agtatccccg ctacctgccg atttccttga acgtcaaccg gtttaccaac tctacacgct
     4441 gttaaatcgt gcaagattat ttggcggtca gcatttggtt attgctcagc agtcattgga
     4501 tagattatta gcagcatgat atgggttgag gataatggcc gctccgtgcg gccttttgat
     4561 taaataaacc caagcagaga aaagaacaca taacctgcag caatacaaat aaccggcagg
     4621 atatatagtg agaaaaactg caggaaaata gtatggtgcg gaacaacaat acgggactca
     4681 atttgttcac gcgttagccc ctcttcccct ttggcttttt ccagaatgag ttgatcttca
     4741 acaccttccc gcaagaagcg cgcctggcga ctcatccggg caccggaatc ctgcaacgcc
     4801 agtccgacaa aaatcaggat gaaaatcacc cagaacataa cgttcacgcc accattaaaa
     4861 ttaggggtcg gggagttata ccagaacaga ttcaaaaaag gcgtattcgc ctgcatcata
     4921 tcgatcatga catgagtaaa gtcgagcatg accgcattaa tgccttcctg gttttcctgc
     4981 cgggtattca taaatttcag caaagaaatc aatgtggaga tcaatgcagg tataaaaatc
     5041 acccaaccca aaatcctttt caaaacagca atgcgtccag cttgttgata cgtcatgagt
     5101 tctccttgat taagacgcgt cgtttcactt agtttacctg tagatatctg ttttcgccca
     5161 ttctttaaag gcgatatgat aggcgcttaa tcataagcac ggcttaatac cttacacata
     5221 atgctctaaa ggagaggttg taatgtcaac cccgcgtcag attcttgctg caatttttga
     5281 tatggatgga ttacttatcg actcagaacc tttacgggat cgagccgaac tggatgtgat
     5341 ggcaagcctg ggggtggata tctcccgtcg taacgagctg ccggacacct taggtttacg
     5401 catcgatatg gtggtcgatc tttggtacgc ccggcaaccg tggaatgggc caagccgtca
     5461 ggaagtagta gaacgggtta ttgcccgtgc catttcactg gttgaagaga cacgtccatt
     5521 attaccaggc gtgcgcgaag ctgttgcgtt atgcaaagaa caaggtttat tggtgggact
     5581 ggcctccgcg tcaccactac atatgctgga aaaagtgttg accatgtttg acttacgcga
     5641 cagtttcgat gccctcgcct cggctgaaaa actgccttac agcaagccgc atccgcaagt
     5701 atatctcgac tgcgcagcaa aactgggcgt tgacccactg acctgcgtgg cgctggaaga
     5761 ctcggtaaat ggcatgatcg cttctaaagc agcccgcatg cgttccatcg tcgttcctgc
     5821 gccagaagcg caaaatgatc cacgttttgt attagcaaac gtcaaactct catcgctgac
     5881 agaactcacc gcaaaagacc ttctcggtta atgaccaggg gcagtgatca cgctgcccct
     5941 ggttctttat ctgaatttgc attcaactga cggattaatc gtcaatttaa gagaaagagt
     6001 tacaccgtca ccacttccgt gcactgtata aaaatcctat actgtacgta tcgacagttt
     6061 agtgagtttt atcatgacgg cggaaggtca ccttctcttt tctattgctt gtgcggtatt
     6121 tgccaaaaat gccgagctga cgcccgtgct ggcacagggt gactggtggc atattgtccc
     6181 ttccgcaatc ctgacgtgtt tgttaccgga catcgatcac ccaaagtcgt ttcttgggca
     6241 gcgattaaaa tggatatcaa aaccgatcgc ccgcgctttt gggcatcgtg gttttaccca
     6301 cagtctgctg gcggtatttg cgctgctggc aaccttttac cttaaggttc cggaaggctg
     6361 gttcattccg gctgatgcgc tacaaggaat ggtgctgggt tatttgagcc acatacttgc
     6421 cgatatgctg acacccgccg gtgttcccct gctctggcca tgccgctggc gtttccgctt
     6481 gcctatcctg gttccccaaa agggcaacca actggaacgt tttatctgca tggcattatt
     6541 tgtctggtcg gtatggatgc cccattcatt acccgagaac agcgctgttc gttggtcatc
     6601 gcaaatgatc aataccttgc agatccagtt tcatcggctt attaagcatc aggttgaata
     6661 ctaaaaaggc aaaaatcacc tttctggaat aagcaattcc atttgaatat aagagccagc
     6721 tcacagttct gttaatcttg cgccaacact atgactgcta cgcagtgata gaaataataa
     6781 gatcaggaga acggggatga actttccatt aattgcgaac atcgtggtgt tcgttgtact
     6841 gctgtttgcg ctggctcaga cccgccataa acagtggagt ctggcgaaaa aagtgctggt
     6901 gggtctggtg atgggtgtgg tttttggcct tgccctgcat accatttatg gttctgacag
     6961 ccaggtactt aaagattctg tacagtggtt taacatcgtt ggtaacggct atgttcaact
     7021 gctgcaaatg atcgttatgc cgttagtctt cgcctctatt ctgagcgcgg ttgcccgtct
     7081 gcataacgca tctcagttag gcaaaatcag ttttctgacc atcggtacgc ttttgtttac
     7141 cacgctgatt gcggcgctgg tcggtgtgct ggtcaccaac ctgtttggtt tgacggctga
     7201 aggtctggtt cagggtggtg cagaaactgc acgtctgaac gccattgaaa gtaactatgt
     7261 tggtaaagtc tctgatctga gcgttccgca gctggtcttg tcctttatcc cgaaaaaccc
     7321 gtttgccgat cttaccggag ccaatccgac gtcaattatc agcgtggtaa tttttgccgc
     7381 attcctcggc gtagctgcat tgaaactgct gaaggatgat gcgccgaaag gtgaacgcgt
     7441 cttagccgct atcgataccc tacaaagctg ggtgatgaaa ctggttcgcc ttgtcatgca
     7501 gttgacccct tacggcgttc tggctctaat gaccaaagtg gttgcaggtt ctaacttgca
     7561 agacatcatc aaactgggaa gtttcgttgt cgcgtcctac ctcggtctgc tgattatgtt
     7621 tgcagtgcat ggcattctgc tgggcattaa tggcgtgagt ccgctgaagt acttccgtaa
     7681 ggtatggcct gtgctgacgt ttgcctttac cagccgttcc agtgctgcgt ctatcccact
     7741 gaatgtggaa gcacaaacgc gtcgtctggg cgttcctgaa tccatcgcca gtttcgccgc
     7801 ctctttcggt gcaaccattg gtcagaacgg ctgcgccggt ttgtatccgg caatgctggc
     7861 ggtgatggtt gcgcctacgg ttggcattaa cccgctggac ccgatgtgga ttgcgacgct
     7921 ggtcggtatt gttaccgtta gttccgcagg cgttgccggt gtcggtggtg gtgcaacttt
     7981 cgccgcactg attgtgctgc ctgcgatggg cctgccagta accctggtgg cgctgttaat
     8041 ctccgttgaa ccgcttatcg acatgggccg tacggcgcta aacgttagtg gctcgatgac
     8101 agctggcacg ctgaccagcc agtggctgaa gcaaaccgat aaagccattc tggatagcga
     8161 agacgacgcc gaactggcac accgttaatt cttatgctgg caacggtccg ttttgtatag
     8221 gggccgttgc tttacttttt aaatgttcca tcgcggcgat gcatactgac tcatatttaa
     8281 acagatcgtc agcgaaaaaa atgtccaact tatcaataca ttcctggctc aatgaatgtg
     8341 cctgtagtga gaagcttaaa acctcatacc cgagacgttt tctaagtgaa ctatctggaa
     8401 tactcataat atcttcaagc acaccacttc ccattcccgg aagatttagt ggattaaaaa
     8461 gaagattgat attaagcatt gatattatcg aaggaaactt taggagactc tcattttttt
     8521 tgaatcgcag caaaaatgct cccggcagga tgtatataat ttttttcata gtatatagtc
     8581 cttaaataat gaataaccaa cctatttttt atattgatca aattcgtcat tttttcgggt
     8641 cggaattcac aggtaaaatc catcccaaca aaattctcat taatatctct tatccgaaac
     8701 tcactgccat aaagattatt aattatcact ctatatttaa cattgtcctg cagatacgta
     8761 tcaaaactaa gtgctggagc tcctggcttt gaggcatcat agttaatgtt agacatatag
     8821 gcaatcatac gattgaagtt cgactctgct atttcatcgc agtcacattg acccgttgat
     8881 ataaagaaag tgagtgccgc catggtagac tcaagccttt ctttaaaaga gtcgaaagcc
     8941 aggcttttaa tatttaaatc accataatta ctctgtatta agtttgtaga aaacatctcc
     9001 cgccccatat tgttaacaaa attattatct catttaaatc taagtcactt acaatataag
     9061 tttaagagcg acgccacagg atgaactatc aaaaatagct catcatgatt agcaaaactt
     9121 aaccatttta aaataaataa acaattaaag aaaaaagatc acttatttat agcaatagct
     9181 cgtcaaaggc agctttttgt tacaggtggt ttgaatgaat gtagcaacga aatgcagaat
     9241 ttcaggtcat gtaactcccg gcaaaaccgg gaagtatgta atccttactc agtcacttcc
     9301 ccttcctggc ggatctgatt tgcccaacgt tgggcagatt caggcacagt aaacgccggt
     9361 gagcgcagaa atgactctcc catcagtaca aacgcaacat atttgccacg cagcatccag
     9421 acatcacgaa acgaatccat ctttatcgca tgttctggcg gcgcgggttc cgtgcgtggg
     9481 acatagctaa taatctggcg gttttgctgg cggagcggtt tcttcattac tggcttcact
     9541 aaacgcatat taaaaattag aaaaactgta gtttagccga tttagcccct gtacgtcccg
     9601 ctttgcgtgt atttcataac accgtttcca gaatagtctc cgaagcggga tctggctggt
     9661 ggtctatagt tagagagttt tttgaccaaa acagcggccc tttcagtaat aaattaagga
     9721 gacgagttca atgtcgcaac ataacgaaaa gaacctacat cagcaccagt caccactaca
     9781 cgattccagc gaagcgaaac cggggatgga ctcactggca cctgaggacg gatctcatcg
     9841 tccagcggct gaaccaacac cgccaggtgc acaacctacc gcccctggga gcctgaaagc
     9901 ccctgataca cgtaacgaaa aacttaattc tctggaagac gtacgcaaag gcagtgaaaa
     9961 ttatgcgctg accactaatc agggcgtgcg catcgccgat gatcaaaact cactgcgtgc
    10021 cggtaaccgt ggtccaacgc tgctggaaga ttttattctg cgcgagaaaa tcacccactt
    10081 tgaccatgag cgcatcccgg aacgtattgt tcatgcacgc ggatcagcag ctcacggtta
    10141 tttccagcca tataaaagct taagcgatat caccaaagcg gatttcctct cagatccgaa
    10201 caaaatcacc ccagtatttg ttaggttctc taccgttcag ggtggcgctg gctctgcaga
    10261 taccgtgcgt gatatccgtg gttttgccac caagttctat actgaagagg gtatttttga
    10321 cctcgttggc aataacacgc caatcttctt tatccaggat gcgcataaat tccccgattt
    10381 tgttcatgcg gtaaaaccag aaccgcactg ggcaattcca caagggcaaa gtgcccacga
    10441 tactttctgg gattatgttt ctctgcaacc tgaaactctg cacaacgtga tgtgggcgat
    10501 gtcggatcgc ggcatccccc gcagttaccg caccatggaa ggcttcggta ttcacacctt
    10561 ccgcctgatt aatgccgaag ggaaggcaac gtttgtacgt ttccactgga aaccactggc
    10621 aggtaaagcc tcactcgttt gggatgaagc acaaaaactc accggacgtg acccggactt
    10681 ccaccgccgc gagttgtggg aagccattga agcaggcgat tttccggaat acgaactggg
    10741 cttccagttg attcctgaag aagatgaatt caagttcgac ttcgatcttc tcgatccaac
    10801 caaactgatc ccggaagaac tggtgcccgt tcagcgtgtc ggcaaaatgg tgctcaatcg
    10861 caatccggat aacttctttg ccgaaaacga acaagcagca ttccatccag gtcatattgt
    10921 tcctggtctg gacttcacca acgatccgct gttgcaggga cgtttgttct cctataccga
    10981 tacacaaatc agtcgtcttg gtgggccgaa tttccatgag attccgatta accgtccgac
    11041 ctgcccttac cacaacttcc agcgtgatgg catgcatcgt atggggatcg acactaaccc
    11101 ggcgaattac gaaccgaact cgatcaacga taactggccg cgcgaaacac cgccgggacc
    11161 gaaacgcggc ggttttgaat catatcagga acgcgtggaa ggaaataaag ttcgtgagcg
    11221 cagtccatcg tttggcgaat attattccca tccgcgtctg ttctggctaa gtcagacgcc
    11281 attcgagcag cgccatattg tcgatggttt cagttttgag ttaagcaaag tcgttcgtcc
    11341 gtatattcgt gagcgcgttg ttgaccagct ggcgcatatt gatctcactc tggcccaggc
    11401 ggtggcgaaa aatctcggta tcgaactgac tgacgaccag ctgaatatca ccccacctcc
    11461 ggacgtcaac ggtctgaaaa aggatccatc cttaagtttg tacgccattc ctgacggtga
    11521 tgtgaaaggt cgcgtggtag cgattttact taatgatgaa gtgagatcgg cagaccttct
    11581 ggccattctc aaggccctga aggccaaagg cgttcatgcc aaactgctct actcccgaat
    11641 gggtgaagtg actgcggatg acggtacggt gttgcctata gccgctacct ttgccggtgc
    11701 accttcgctg acggtcgatg cggtcattgt cccttgcggc aatatcgcgg atatcgctga
    11761 caacggcgat gccaactact acctgatgga agcctacaaa caccttaaac cgattgcgct
    11821 ggcgggagac gcgcgcaagt ttaaagcaac aatcaaggtc gctgaccagg gtgaagaagg
    11881 gattgtggaa gctgacagcg ctgacggtag ttttatggat gaactgctaa cgctgatggc
    11941 agcacaccgc gtgtggtcac gcattcctaa gattgacaaa attcctgcct gatgggaggc
    12001 ggcgcaattg cgccgcctca aatgatttac atagtgcgct ttgtttatgc cggatgcggc
    12061 gtgaacgcct tatccggcct acaaaactgt gcaaattcaa tatattgcag gaaacacgta
    12121 ggcctgataa gcgaagcgca tcaggcagtt ttgcgtttgt cagcagtctc aagcggcgca
    12181 gttacgccgc ctttgtagga attaatcgcc ggatgcaagg ttcacgccgc atctggcaaa
    12241 catcctcact tacacatccc gataactccc caaccgataa ccacgctgag cgatagcacc
    12301 tttcaacgac gctgatgtca acacatccag ctccgttaag cgtgggaaac agtaagcact
    12361 ctgacggata gtattatcga taaacgccgg atgacacatc acctccagcg aacgatcacc
    12421 ccgatggctt gcgtcatcca gcacttgcag gaacagtgcc tcgctaatct cttcaccata
    12481 aaacgcgcta ctgaatccct gagaactgcg cagattagcc ggtaaatcac cagagttaga
    12541 tagcggctga cggtcgatgc gcaacgcaat cccctcttca gccgcaaacc tggcaacaat
    12601 cgggaaaatc tgcgggaaca tatgcacatg atgatggcta tcaagatgcg taggtttgcg
    12661 tccaaatagc tcaatgaaac gcaaatactg actggcaagc tcctgagtaa tttcttccag
    12721 cggtaaagca tcttcttctg ccaactgcca gatccatttt cccagcacac catcgcgggt
    12781 taaccccggc atagctgtca gtggcttgcc catagtaagg acaaagtaca tccctatggc
    12841 cagactcggt tcatcacgac tcaactgcac cgcatggtca atagcctgcc cattcaccag
    12901 cgccgtcgtc gatgtgacaa tcccattgcg acaggcctcg ataatgccgt agttctgacc
    12961 tttgcttaag ccaaaatcat cggcattaac aatcagtaag cgttccataa tcagcctcgg
    13021 ttaatgtgct tttttaagct ctgcgatgca gtcggcaaaa tttggcagcc atttctcgtg
    13081 cgccagaatc atctcgcgtg ccagcagctc agcatcgcga tcggaatgca ccaacggact
    13141 aaggtttagc gccagtaaca catcgttaaa ttctccgcta agtgcggcgt tgctggcagc
    13201 aatctcgaag cctttaatgg tgtgaatcag ccccatcact ttatcatcga aatgcgtaat
    13261 gcgcggatgt ggcgtcgcgc catcgcgccc cagcttacag gtcatttcta ccgcccagtc
    13321 tgccggaata ttatcaatat gcccatgatg cgggatatta acgtaatgtt cagcttgctt
    13381 gtcgttgtag atagcgttga tcacttcgca cgctgcatca gagtaataag ccccaccgcg
    13441 ctgttccagt tctttcggct taactttcaa ctccgggttt ttatacagct caaaaagttg
    13501 tttctcgact ttctgtacta cctgcgctcg tgcgccgcct ttgtagtatt cgcccatttc
    13561 aatagccagc atctcttttt gcttgaagta ataaagcaaa taagaacacg gcaacagatt
    13621 cagagaacga attaagcctt cgctaaatgg cagatcgaaa atatttttaa cgccagatgc
    13681 ttttaactgc ccggatgcca caccatcaag caattcggca aagcgcgact tgccatttac
    13741 cagcacatcc ttaatgaaca ccatatggtt gaggccgaac aaatcgatag ataaatcatc
    13801 gctgtctttc agcatcagaa catcgtgaat aaacatcttc atgccgatcg gaatattaca
    13861 cacgccgata aagcgtttaa atccggtatg acgataaacg gcttcagtaa ccattcccgc
    13921 cgggttagtg aagttaatca cccatgcatt cggacaaagt tcttcgacat cttttacgat
    13981 gtcaaaaatc accggaatgg tacgcagacc tttaaacaga ccgcccgcgc cgttggtttc
    14041 ctggccaaga taaccatgac ttaatggaat acgttcatcc agttcgcgcg ccggtaattg
    14101 gcctacgcgc agttgggtag taacgaaatc agcatctttc aatgcttcgc ggcgatccag
    14161 cgttttataa agcttcatcg ggacgccagc gttatcaatc atccgttggc agagttcaaa
    14221 aatgatatcc agtttctctt taccatcttc gacatccacc agccataatt cgctgaccgg
    14281 caattcgtga taacgcttaa taaatccttc cagtaactcc ggggtatagc tgctcccgcc
    14341 accaatagtg acgactttta atttctggct cataatttct cccttcagta cagaatactg
    14401 atatctggca tatctgcccc cccggacata aataatccag caacaggaca gatatgtgaa
    14461 ttgtcaggta taacgactta ctgattaaat tcagtcaatt tcttacgata gctcttaggc
    14521 gtaaaggacg ttaatttctt aaacgtttta ataaacaaac tcgggctact ataaccggcc
    14581 tcaaacgcaa tatccgtcac ggaatagttg gtcatttcca gttgtttttt ggcaaaatta
    14641 atacggatct cattaataat ctgcattggt gttttgccat aatatcgttg agtcgctcgc
    14701 gtcaaatatt cctgtgattt ggctgacaac gtcaccatat tctccagcgc cgattcacta
    14761 aactgctctt tatcatgcat cttttctacc gtacttttca gccactgcgg tacatcatca
    14821 atcacctgtt cttcgcggta atgacgtaaa cggttaatga cataaaaagt aaccatctca
    14881 acaaactctt ccagccctgt ttcgcggaaa ttcaatgaag aaatcactgt ttccacatag
    14941 gtgagaaaag cgttattggt ccggtatacc tgcgaagcga caaagcaata aggcaacaac
    15001 ggcaggtaat gctgctcaaa aaagcgttta ctgatcccaa cgttcaatat acgcgtggca
    15061 ccaaactcat aaaaactttg atggtgcgaa cctaacggaa taaaaacaaa atcgccccgt
    15121 tccagtaaca cgcgcttacc gttaatctcc tggaaataac gcccggttaa taccagagta
    15181 aattcataat agtcgtgctg atgcagcccg ctgatactct cagttttgtt atagataaac
    15241 acatggaagt ttttgccatt aaacaactgc tgttctcggg cagtggcaat ttccggcgcg
    15301 ttaatcactg gctgcatcat cgactcctta tgccttcagt ttttcatgaa gctcaattaa
    15361 ttcagtaatc agttcacgcg caagcatgga cgtcattaaa tgatcctgag cgtggaccag
    15421 caccagactc actttcatct taccttcgcc cgcatcgcct tcaatcagtt tcgtctgtac
    15481 cagatgcgct tcattcaatg ccatccgtga ctgatccatc atggcttttg ctgcagcaaa
    15541 atcgccctgc ttcgcctgtt tcagtgccgc atacgccagg ctgcgcgctt gtccggagtt
    15601 gatgataagc cccatcacca cttcttccag ctcttcagct tccgtttgcg tatcgggaat
    15661 gttatcgaga tccatcatac atcgttcctc tctattctta ccggcacgat tacccgtacc
    15721 ggcatcgatt aaaatttcag ggcgttagcg atatcttctt cgcgctcttc tttatcaatc
    15781 gcgttttgtg ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatacca
    15841 aggttgaaga gtgcaaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc
    15901 agaccggttg gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag
    15961 tacgctgcga gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc
    16021 accgggttca tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg
    16081 gacggcagcg ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata
    16141 gccaggatca ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc
    16201 ggcttggccc agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat
    16261 tgctgatagg tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg
    16321 gtcagcgcca gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag
    16381 gcccagccca ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc
    16441 tgatggaagt tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga
    16501 ataataaagc cgggaattaa tgccgagaag gaacgcgata ctgaagcagg tacgctatcg
    16561 ggcaatttaa tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg
    16621 ccaataataa tcccggagat gatattcgct ccacctaacc agtttgcgcc aaccacatag
    16681 gcctcaccga cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc
    16741 gccagcgcat cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt
    16801 aaagacatta tccctaatgt tccgttatat acgttgccgc caataccttt caggccatta
    16861 agtgtttcaa tggttgaggc gtcgaggcga atacctaagg aataaaaaaa cgccccctcc
    16921 ccaaagctta gaaaaacgtt gttaattaat acaaacatgg cccccgcaag ggttaacggc
    16981 attaagcgaa taaagccatt tttgattgca ttaacgtgtg gctgctttcc tattttaact
    17041 gcaaaaggga ggagtacctt ttcaagcgat gcaataacat tactcataga aaaataccct
    17101 taaaaaccgc aatttaaata ttgcggtatt gatttatgaa ataactcttt gacgggaaaa
    17161 tttaaaataa attaatttgc tgcggctttt ttaatcgctg caaccgcagc cttaagcacg
    17221 cctaaaccat cgactttgcc ataaagcagc gagtcaatta cttcaaccgg tttgttgggt
    17281 aacaaacgct ggatttcggg caacatataa gcaatctgcg gccctaataa cacgacatcg
    17341 gcattctgac ctttttcacc agccagtgtt tccggaaatg cttcaataat gaccggaact
    17401 tcatattttt ctgcctgtgc gcgcattttt gataccagta aagaggtaga catgcccgca
    17461 gaacaaaaca gataaatgtg tttcttttcc ataaaactgc cctcatcgac gattatctgt
    17521 cagccagaca ctccgcaagc cttaacctgc ttccatgctc tgggtaactt gcgaaaccaa
    17581 catgactttt tttgtggctg aaaggagtat acggtataga cctgagcaat agtatttctt
    17641 tgaccttata aattgtttct ccttgacctg tggttatgac cctcttcaca tttcgggcaa
    17701 ataattcgcg aagataatta agaactcagg ccaaaaaaaa ccggcgcaat ggccggtttc
    17761 cgttgttact caagctttca gacgaattga ttacttcgca gcctgtggat cagtgtcgta
    17821 ttcagcacag gtctgataac cggagttgat gacatgtccg gtatcatcta acgcgacaaa
    17881 gtaggtttct gctttaccat cacgttgacc caggatgtag gtctggcaag taccacgagc
    17941 atggatcatg ctcacttcag acgaaggttt acccgcaatt tgtgcaacct gcgcccggct
    18001 catgcctttt ttgacgtctt tcaccacagg ctgtacaaac tggtctttag tacgatcata
    18061 agccgtacaa cccgccagca tggttaatac cgccgctgca ctcagaattc ctgccatatt
    18121 cttgttcata ttccgtcctc ttgtttatca gcgtgttaga taagcctgga atacattggg
    18181 cgctttttca agcccgcgaa cgaaacggct ccgctttcag aggattcctg tatgacgttt
    18241 taaccaccat tcagcccgct gtcgcttgtc gtttcagtgg caacgggtta gctttaagga
    18301 agttttgtct tttctgtctg gaggggttca atgacattgc aacaacaaat aataaaggcg
    18361 ctgggcgcaa aaccgcagat taatgctgaa gaggaaattc gtcgtagtat cgattttctg
    18421 aaaagctacc tgcaaactta tccgttcatt aaatcactgg tgctcgggat cagcggcggt
    18481 caggactcca cgcttgccgg aaagctgtgc cagatggcga ttaatgagct gcgcctggaa
    18541 accggcaacg aatcactgca atttattgcc gtacgcctgc cctatggtgt tcaggccgac
    18601 gaacaagatt gccaggatgc cattgccttt attcaaccgg atcgcgtatt aaccgttaat
    18661 atcaagggcg cggtattggc cagcgaacag gcattgcggg aagcaggcat tgaactgagc
    18721 gattttgtcc gtggcaatga aaaagcgcgt gagcggatga aagcacaata tagcattgcg
    18781 ggtatgacca gcggtgtcgt ggtgggcacc gatcatgcag cagaagccat taccggattc
    18841 ttcactaaat atggtgacgg cggtacagac attaatccgc tgtatcgtct caacaaacgt
    18901 cagggtaaac agttactggc ggcattaggt tgcccggaac acctttataa gaaagcgcca
    18961 acggccgatc tggaagatga tcgcccttct ctgccagatg aagtggcact cggcgtgacc
    19021 tatgacaata tcgacgacta tctggaaggg aaaaacgtac ctgaacaggt cgccagaaca
    19081 atagagaact ggtatctgaa aaccgaacat aaacgccgtc cgccaattac cgttttcgat
    19141 gatttctgga aaaagtaata atttgcacat aatggtttgt gcgaaaaaga gtaatttgtt
    19201 cacgccggat gcggcgtgaa cgccttattc gacctacaaa actatgcaaa ttcaatatat
    19261 tgcaggagtc gaataggcct gacaggcgta gcacgtcaga cggtgtaacc tttgtcatcg
    19321 acccgcctct tttttaatcg ctccccgcct gttacactgg atagataacc agcattcgga
    19381 gtcaacagtg gtacggcgtt taacttctcc gcggctcgaa tttgaagctg cggcaattta
    19441 tgaatatccc gaacatttac gttcattcct taatgactta cccacccgac caggggtgta
    19501 tctgtttcat ggcgaaagtg acaccatgcc gctctatatc ggcaaaagcg ttaatatccg
    19561 cagtcgggtg ctttctcatt tacgcacccc ggatgaagcc gccatgctac ggcaatcccg
    19621 gcgaattagc tggatctgta ccgcaggcga aatcggcgct ctgctccttg aagcgcgatt
    19681 aatcaaagaa caacagccgc tgtttaataa acggttgcgc cgcaatcgcc agctctgtgc
    19741 cctgcaatta aatgaaaagc gcgtcgatgt ggtgtatgcc aaagaggtgg atttttcacg
    19801 agcccccaac ctgtttggcc tgtttgccaa taggcgcgca gctttgcaag cattgcagac
    19861 catcgctgat gaacaaaaac tttgttatgg cctgctggga ctggaaccgt taagtcgcgg
    19921 tcgtgcatgt tttcgttcag cgctaaaacg ttgcgccgga gcatgctgcg gtaaagagag
    19981 ccatgaggaa catgcgctac gcttgcgcca gtctctggag cgtttgcggg tggtgtgttg
    20041 gccttggcaa ggtgcggtgg cactgaaaga acagcacccg gaaatgactc aatatcatat
    20101 tattcaaaac tggctgtggc tgggggcggt taattcgctg gaagaagcga caacgttaat
    20161 tcggacaccc gccgggtttg atcacgacgg ttataaaatt ctttgtaagc cgctgctttc
    20221 cggtaactat gaaattactg aacttgatcc ggcgaatgac cagcgagcca gttgatttca
    20281 ctgaacaaca gtttaccttg cggttgtaaa agccgcaggg tgtgcctgcc atcgaaccag
    20341 cacgcgccct ggtcggtagt gagcaattta tcgcccagtt gccacgcccc gttaatcaca
    20401 aacaccaccc cgccgcgtga gccaaaggta gtaaacgtac gttcggcaat tctgactttc
    20461 gccttacaga catccaggcg tgtcatgatg ttgaaatcca tcgacatctg cccttccgtc
    20521 agtttcgctt ttaccacctg gtccgctgca aaggcaaaag gctgtaacgg ttttaaggta
    20581 tggttaaagc ggtctgcgct ttcaaggagc atctcgccgc cttccagcaa cgtcactatc
    20641 ctttccatgc cgggaaacaa agaaaactcc ccattcgccg cgatggatgc aatgctggca
    20701 cgccagtaaa aatcacgttt tgccggtggg aaagtgcaaa tttcgcgcgt ttcaccggca
    20761 gcatttcgcc acagattcac cgacatttta cgcatatcaa agtattccat actcgctccc
    20821 ggcccgttct tttcattgtt atcgttcagg taaccgtgga tggttatcgg caactcgggt
    20881 gatttacgtt agtggtgatc aggctttttt tacaactgtt tgagaagaga aaagaaaacc
    20941 gccgatcctg tccaccgcat tactgcaagg tagtggacaa gaccggcggt cttaagtttt
    21001 ttggctgaaa gattattcag cagttgcagg cattttacct tttgcc
//
LOCUS       DAJVRF010000065        21003 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJVRF010000065 DAJVRF010000000
VERSION     DAJVRF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.065"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..170)
                     /locus_tag="OQN13_002210"
     CDS             complement(<1..170)
                     /locus_tag="OQN13_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1615562.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            342..1562
                     /gene="coaBC"
                     /locus_tag="OQN13_002211"
     CDS             342..1562
                     /gene="coaBC"
                     /locus_tag="OQN13_002211"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCS1615563.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1540..1998
                     /gene="dut"
                     /locus_tag="OQN13_002212"
     CDS             1540..1998
                     /gene="dut"
                     /locus_tag="OQN13_002212"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCS1615564.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDSTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2105..2701
                     /gene="slmA"
                     /locus_tag="OQN13_002213"
     CDS             2105..2701
                     /gene="slmA"
                     /locus_tag="OQN13_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCS1615565.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="OQN13_002214"
     CDS             complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="OQN13_002214"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCS1615566.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3445..4161)
                     /gene="rph"
                     /locus_tag="OQN13_002215"
     CDS             complement(3445..4161)
                     /gene="rph"
                     /locus_tag="OQN13_002215"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCS1615567.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4288..5151
                     /locus_tag="OQN13_002216"
     CDS             4288..5151
                     /locus_tag="OQN13_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCS1615568.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5373..6197
                     /gene="dinD"
                     /locus_tag="OQN13_002217"
     CDS             5373..6197
                     /gene="dinD"
                     /locus_tag="OQN13_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCS1615569.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6489..7106
                     /locus_tag="OQN13_002218"
     CDS             6489..7106
                     /locus_tag="OQN13_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCS1615570.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="OQN13_002219"
     CDS             complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="OQN13_002219"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCS1615571.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEIARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9043..9666
                     /gene="gmk"
                     /locus_tag="OQN13_002220"
     CDS             9043..9666
                     /gene="gmk"
                     /locus_tag="OQN13_002220"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCS1615572.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9721..9996
                     /gene="rpoZ"
                     /locus_tag="OQN13_002221"
     CDS             9721..9996
                     /gene="rpoZ"
                     /locus_tag="OQN13_002221"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCS1615573.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10015..12123
                     /gene="spoT"
                     /locus_tag="OQN13_002222"
     CDS             10015..12123
                     /gene="spoT"
                     /locus_tag="OQN13_002222"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCS1615574.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12130..12819
                     /gene="trmH"
                     /locus_tag="OQN13_002223"
     CDS             12130..12819
                     /gene="trmH"
                     /locus_tag="OQN13_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCS1615575.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12825..14906
                     /gene="recG"
                     /locus_tag="OQN13_002224"
     CDS             12825..14906
                     /gene="recG"
                     /locus_tag="OQN13_002224"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCS1615576.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="OQN13_002225"
     CDS             complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="OQN13_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCS1615577.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16560..17951
                     /gene="xanP"
                     /locus_tag="OQN13_002226"
     CDS             16560..17951
                     /gene="xanP"
                     /locus_tag="OQN13_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCS1615578.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTTLKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18072..19781
                     /locus_tag="OQN13_002227"
     CDS             18072..19781
                     /locus_tag="OQN13_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS1615579.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="OQN13_002228"
     CDS             complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="OQN13_002228"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCS1615580.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5083 a         5713 c         5385 g         4822 t
ORIGIN      
        1 ccataaagag agccgaaatt ctccagcatc tcttttgcca gggttaatac atctttaccg
       61 cgcgttccgg tacgcagaaa tagcgccagc agctcgacat ccgttaaggc gctaatacca
      121 aacttcagca ttttttcgcg cggcatcaac agctgtgcat tgtttttcac cttcacctcc
      181 tttgtggtgc tcgcatcctg tcatagccgt ttcgttaaat cgacggccag ttttcaatcc
      241 tggaaagcgc ctcgcaaagt gaatctcagg tgaaggcacg acaacaaaag tattgtgata
      301 aaatcgccaa cttctggtgt caccctacag gaaaaatcat catgagcctg gccggtaaaa
      361 aaatcgttct cggcgttagc ggcggtattg ctgcctataa aacccctgaa ctggtgcgtc
      421 gtttgcgcga tcgcggggcc gacgtccgcg tagccatgac cgaagcggca aaagccttta
      481 tcaccccact tagcttgcag gcggtttctg gttatcccgt ttccgacagt ctgctggacc
      541 cggcagccga agccgctatg ggccatattg agctgggtaa atgggctgat ttagtgattc
      601 tcgcccctgc cacggcagat ttgattgccc gtgttgctgc cggaatggcg aatgacctgg
      661 tatcgacgat ttgtctggct acacctgcgc ctgtagccgt gctccccgcc atgaaccagc
      721 agatgtaccg tgccgctgcc acgcagcata atttagaggt gcttgcttcc cgtggtttgc
      781 tcatctgggg gccagacagt ggcagtcagg cttgtggtga tatcggtcct gggcgaatgc
      841 tcgatccgtt aaccattgtg gatatggcgg tagcgcattt ttcgcccgtc aacgacctga
      901 aacatctgaa cattatgatt accgccggcc cgacgcgtga accgctcgat ccggtgcgtt
      961 atatctctaa tcacagctcc ggcaagatgg gttttgctat cgccgccgcc gctgcccgtc
     1021 gtggcgcgaa cgtcacgctg gtatcaggcc cggtttcgct accgacgcca ccgtttgtta
     1081 aacgtgttga tgtgatgacc gcgctggaaa tggaagtcgc cgtgaatgct tctgtacagc
     1141 agcaaaatat ttttatcggc tgcgccgccg tggcggatta tcgcgcagct accgtggccc
     1201 cagagaaaat caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa
     1261 accccgatat cgtcgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat
     1321 ttgccgccga aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc
     1381 ttgatctgat ctgcgcgaac gatgtttccc agccaactca aggatttaac agcgacaaca
     1441 acgcattaca ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc
     1501 tccttggcca attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt
     1561 aagattctgg acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc
     1621 tctgccggac ttgacctgcg tgcctgtctc gacgacgccg tagaactggc tccgggtgac
     1681 tctacgctgg ttccgaccgg gctggcgatt catattgccg atccttcact ggcggcaatg
     1741 atgctgccgc gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga
     1801 ttgatcgatt ctgactatca gggccagttg atgatttccg tgtggaaccg tggtcaggac
     1861 agcttcacca ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag
     1921 gctgaattta atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt
     1981 cactctggtc gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat
     2041 ttcgtttgcg gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg
     2101 taacatggca gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct
     2161 ggcgctgatg ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc
     2221 ctctgtcggc gtttccgaag cggcactgta tcgccacttc cccagtaaga cccgcatgtt
     2281 cgatagtctg attgagttta tcgaagatag cctgattact cgcatcaacc tgattctgaa
     2341 agatgagaaa gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg
     2401 tgagcgtaat cctggcctga cccgcatcct cactggtcat gcgctaatgt ttgaacagga
     2461 tcgcctgcaa gggcgcatca accagctgtt cgagcgtatt gaagcgcagc tgcgccaggt
     2521 attgcgtgaa aagagaatgc gtgagggtga aggttacacc accgatgaaa ccctgctggc
     2581 aagccagatc ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa
     2641 ataccgcccg acggatgatt ttgacgcccg ctggccgcta attgccgcgc agttgcagta
     2701 atatgatgcc ggatgacttt tcatccggcg agtttcttta aacgccaaac tcttcgcgat
     2761 aggccttaac cgccgccaga tgttccgcca tttccggctt ctcttccagg taagcaatca
     2821 ggtctttcag ggtgatgata gagatcactt tgcagttgta atcacgctca acttcctgaa
     2881 tcgccgaaat ctcgctgcgc ccccgttcct gacgatcgag cgaaatcaac acgccagcaa
     2941 gcgtcgcgcc attggcctga ataatttcca tcgactcacg aatcgccgtt ccggcggtga
     3001 tcacatcatc taccagcatt acgcgtcctt gtaacgcgct accaaccaga ttgccgcctt
     3061 caccgtggtt ttttgcctct ttgcggttaa agcagtacgg caggtccagg tcatgatgct
     3121 ccgccagcgc cacggcggtt gtggtggcaa tcgggatccc tttgtaagca gggccaaaca
     3181 gcagatcgaa ctcaatgccg gaatccacca atgcttcagc gtaaaaacgg cctaacagtg
     3241 ccaggtcgcg cccggtatta aacagcccgg cgttgaagaa ataggggctt ttacgcccgg
     3301 atttcagcgt aaactcgcca aactttaaca cctgcttgct aagcgcaaat tcaataaact
     3361 ggcgctgata tggtttcatg ccttcgctcc tcatcttact tttctacaga caaaaaaaag
     3421 gcgactcatc agtcgcctta aaaatcagtt tgccagcgcc gccttctgcg tcgctacaat
     3481 ggattcgatt ccccctcggg ccagagccaa caaggtgagt aattcttcgt gggtgaacgg
     3541 ttcgccttct gccgtcccct gcacttcaat gatgcgcccg tcttcggtca tcactacgtt
     3601 catgtcggtc tctgcggcag agtcttcaac gtattccaga tcgcaaaccg cttcgccgtt
     3661 cacaatcccg acagaaaccg cggctaccat ccctttcatc ggattggttt tcagcttacc
     3721 gttttccacc agcttctgta gcgcatctgc cagcgccacg caggcacccg taatcgaagc
     3781 ggtacgagtg ccaccatcag cctgaagcac gtcgcagtcc agcgtaatgg tgaactcacc
     3841 cagcgctttc aaatctactg ccgcgcgaag cgcacgggcg atcagacgct ggatttccat
     3901 tgtgcgtcca ccctgcttac ctttcgccgc ttcacgagcg ttacgggtgt gggtagaacg
     3961 cggcagcata ccgtactctg cggtgatcca gccctggccc tgacctttca ggaagcgcgg
     4021 cacgccttct tcaatagagg cggtacacaa cactttggta tcgccaaatt cgaccagcac
     4081 cgagccttct gcatgttttg tatagttacg agtcagggta acgggacgca cctgattatt
     4141 gctacggcct gctggacgca tattgaaatc tccggcttga aacaaatgtg gctgcgcatt
     4201 atacggactt ccggaggtta ttcctatcct gacaaggcat cgatggctat aatccttcca
     4261 cctctccttt tataaacagg aacgtctatg atccgcagta tgaccgccta cgcccggcgt
     4321 gaaatcaagg gtgaatgggg gagcgcaacc tgggaaatgc gctcggtaaa ccagcgttat
     4381 ctggaaactt actttcgtct gccggagcag ttccgtagcc ttgaacctgt cgttcgcgag
     4441 cgtattcgtt ctcgcctgac gcgcggtaaa gtggaatgta ccctgcgcta tgagccagat
     4501 gttagcgcgc aaggtgagct gatcctcaac gaaaaactgg ctaaacagct ggtaactgcc
     4561 gcgaactggg taaaaatgca gagtgacgaa ggggaaatca acccggttga tattctgcgc
     4621 tggccgggcg taatggcagc ccaggagcag gatctcgacg cgattgccgc tgaaattctc
     4681 gcggcgttgg atggtaccct ggacgacttt atcgtcgcac gtgaaaccga aggtcaggcg
     4741 ctgaaagcgt tgatcgagca gcgtctggaa ggcgtcaccg ccgaagtggt caaagtccgc
     4801 gcccatatgc cggaaatcct gcaatggcag cgtgagcgtc tggtcgcgaa gctggaagat
     4861 gctcaagtgc aactggaaaa caaccgcctg gagcaggagt tggttctgct ggcacaacga
     4921 attgatgttg ccgaagaact ggaccgcctg gaagcgcatg tcaaagagac ctacaacatt
     4981 ctgaagaaaa aagaagccgt tggtcgtcgt ctggacttta tgatgcaaga gttcaaccgc
     5041 gagtcgaaca ctcttgcgtc gaaatctatc aatgccgaag tgacgaattc tgccatcgag
     5101 ctgaaagtgt tgattgagca gatgcgcgag cagattcaga atattgagta actcgtaaca
     5161 ctaattctta cctttcagcc ttcacaaatg ccgcccactc aaacagagcg gcatttttct
     5221 tccccgcaac attcaattct gttttgcgta cctgctccag attttgcgat gtttttttgc
     5281 gcagcgcagt gagaacgtga gatactcaca actgtatata aatacagtta tagacttact
     5341 ttctttgcaa tcgatatcac atggagtggg caatgaacga acatcatcaa ccttttgaag
     5401 aaatacggca ttatggcact gaagggcaag aattttggtc agcgagagaa ttggcaccgt
     5461 tactggatta tcgggactgg cgcaattttc aaaaggtact ggcccgcgcg actcaagcgt
     5521 gcgaagccag caatcaagct gcatctgacc atttcgttga aaccaccaaa atggttgtat
     5581 taggttcagg ggctcaacga gaacttgaag atgttcatct ctctcgctat gcctgttacc
     5641 tggtagtaca aaacggcgac cctgcgaaac cggttattgc ggcagggcaa acttattttg
     5701 ctatccagac ccgacggcag gagctggctg acgatgaagc tttcaggcaa cttcgtgaag
     5761 acgaaaagcg tctttttctg agaaatgagt tgaaagaaca taacaaacaa ttagttgagg
     5821 cagcgcagca agctggggta gcgacagcta tcgatttcgc catatttcag aatcatggtt
     5881 accgggggct gtatggtgga ttagatcaga aagccatcca tcagcggaag gggctgaaaa
     5941 agagtcagaa gatcctggat catatgggtt caacagaact ggcggctaat ctctttcgag
     6001 ctacccaaac agaagaaaaa ctcaagcggg atggcgtaaa ttcaaaacag caagctaata
     6061 ccacacactt tgacgtgggt agcaaggtga ggcaaaccat tcaggaactt ggcggaacca
     6121 tgcctgagga gttgccgacc ccgcaggtca gcatcaatca gttggaaaac agtgtaaaaa
     6181 ttacagagaa gaaatagcag aaactaaaaa ctctttttct taattgagat acccgatgcg
     6241 taagccaaag tccaggtgca gtgaacacaa tggctaaaca ttgcaccttt cttttccacc
     6301 cctcaatttt aacctatttt ttcttatgca ttttctcaga caagaagtca gaagaatgca
     6361 tctacactac agaaaatagc gatttcacat aactacaagt tatcaatttc ctctcacctt
     6421 aaaaaatctc aatcgtgaca atacgcacaa atcgctaccc tgccagacag atttttaggg
     6481 agagaaccat gctgttacac attttgtatt tggttggcat tactgccgaa gccatgacgg
     6541 gggcgctggc ggccggacga cggcgcatgg atacatttgg cgtaattatt attgcgaccg
     6601 ccaccgcaat tggcggaggg tcagtgcgcg atattctgct gggccactat ccgctcggtt
     6661 gggtcaaaca cccggaatat gtgattatcg tcgctaccgc cgcagtactc accactatcg
     6721 ttgctcctgt aatgccttac ctgcgcaaag tgtttctggt gctcgatgcg ctcggactgg
     6781 tggtcttttc tatcatcggc gcacaggttg cactggatat ggggcacggc ccaattattg
     6841 ccgttgtcgc ggcggtaacc accggcgtgt ttggcggcgt tttgcgcgat atgttctgta
     6901 aacgcatccc actggtattc cagaaagagt tgtatgccgg tgtctcattt gcctccgccg
     6961 tgctgtacat cgcgctgcaa cactatgttt ctaaccatga tgtggtgatt atctccaccc
     7021 tggtattcgg cttttttgcc cgcttactgg cgctacgtct taagctggga ttaccggtct
     7081 tttactacag ccacgaaggt cactaaggtt caaaacctgt gatctgctgg gcagccagcc
     7141 aactgcccag cttcttgatt tgcgcatttt ccttccattc aataacctgt ctggcgcgcc
     7201 cagatccagt ccccggcagc cgctgccaga actgctccgt gctaaataaa agttgcgacc
     7261 aggaccgttc atcactggca ttaagcgccg cccgggttaa cggtattccc attgccatca
     7321 cccagcgagt aaaaggctgc ttacgagcca gattaaactg atgccataac tgcgcacttt
     7381 tacttttcgc gatccccggc gtgttctgta attgctctgg cgttaataaa agccaggaaa
     7441 agatatgctc aaagcgatga gtctgatgca gcgcgcgcca gccggcctca ccaatgccat
     7501 ccagcccaag aacctgtttt gaccccagcc agactaagcg tgaaatgaac tgttcctgac
     7561 aaacatcaga agcaaagtag caggtcaacg agttaaagcg gttttctggt ggtgtcggtt
     7621 ttgtacgttc tgcaccgcgc cagaccacat catcaatgcg aggaataccc tgaccggcaa
     7681 ggctgacgag gatctgatca ccaggcgcaa tatcccactc ctgccagcgc ctgacggaac
     7741 caatattcac ccgctggact tttttatcat ccagcatgac aggtgcgagt gacgcaacca
     7801 ccgatatttt accgctctta cccaccgcaa actgaatcgc cttcacttcg gcaacctgag
     7861 ctacaggttg atatttccag gccaccaacc attctgcctg gcctggtagc caatggcggg
     7921 attcgggttc cttagccgct cgcacaacca cgccatcggt gacgaagggt aatttcgctt
     7981 tccaccactc attgcgtacg cgcgcaattt catcagcatt tttaaccgca cgggtatacc
     8041 tctgcgttag agtaaaacct gcggtagcca gctcctttaa acgatcggtc attaactgcg
     8101 gtccatccgg ccatgcccag acaaaaacag ccagtgagtt tagcgtgtcg ctattcccct
     8161 ggcgcatcat caggccagca acttttgcgc gggcatttat tccccccatt tgttgttgga
     8221 tatgcccctc gcgctggaga aatatttccc cctgaagcgt actgttggct aaaggcccgc
     8281 taacggtttg cggcacagcg gaaattaagc taactttctg cgtccagtcc tcgcctttca
     8341 ggccattacc gcgactgatt gctttgttca gtttcccgtc ccgataaacc agggttaccg
     8401 caacgccatc aacttttggc tgcacccaaa gatcgctacg ttctcgcatc cacagactta
     8461 atgcgttttt atccaccatt ttgcgtacac ccgtatgggc aaccggatgc ataaccgcac
     8521 catttaaagg cggcatcatc acgtcgcggg gctcgctccc aaagcagcgt tgccactgcg
     8581 taagacgggc gcttaactga tcgtaaacac cgtcttccac ctcacttttc ccttccttcc
     8641 agtagtcatc gtcccactgt tttatttgct gttgcaggcg ggaaatttct tcctgtgctc
     8701 tggctggcga ccaggccgga cagaccgccc acacagatga ttgccagcac aagatactta
     8761 ttaatatcgc catccatact ttcatcatca cctccgctgt agatagtcag gcagatatac
     8821 aacgtgatga aagcaaagcc gagtggcaaa aacggagtct gcgaggacgc ttcctgaaaa
     8881 tcgtctttat tgcaatgaat gacaggcaaa tgcggaagca gctacgcaaa acgcaacaac
     8941 tttgcgcaaa aagtgtgagc aagggctacg tcacatggcc gcgccgtgta taataagctc
     9001 gtatgtaggc tttatttcgc taatcacata cgaaagatac tcatggctca aggcacgctt
     9061 tatattgttt ctgcccccag tggtgcgggt aaatccagcc tgattcaggc tttattaaaa
     9121 acccaaccgt tgtatgacac ccaggtttct gtttcacaca ccacacgcca accgcgtcct
     9181 ggtgaagtcc acggtgaaca ttatttcttt gttaatcatg atgaatttaa agaaatgatt
     9241 agcagagatg cgttcctcga acacgcagaa gtttttggta attactatgg cacttcgcgt
     9301 gaggccattg agcaagtact ggcgaccggt gtcgatgttt ttctcgatat cgactggcag
     9361 ggcgcgcagc aaattcgcca gaagatgccg cacgcgcgga gtatctttat tttaccgccg
     9421 tccaaaattg aactggaccg ccgtctacgc ggtcgcggtc aggacagcga agaggtcatt
     9481 gcaaagcgta tggcgcaagc tgttgcagaa atgagccatt acgccgaata tgattatctg
     9541 attgtgaatg atgacttcga taccgcgttg accgatttga agaccattat tcgcgccgaa
     9601 cgtctgcgca tgagccgcca aaagcagcgt catgacgctt taatcagcaa attgttggca
     9661 gactgaacct gatttcagta tcatgcccag tcatttcttc acctgtggag ctttttaagt
     9721 atggcacgcg taactgttca ggacgctgta gagaaaattg gtaaccgttt tgacctggta
     9781 ctggtcgccg cgcgtcgcgc tcgtcagatg caggtaggcg gaaaggatcc gctggtaccg
     9841 gaagaaaacg ataaaaccac tgtaatcgcg ctgcgcgaaa tcgaagaagg tctgatcaac
     9901 aaccagatcc tcgacgttcg cgaacgccag gaacagcaag agcaggaagc cgctgaatta
     9961 caagccgtta ccgctattgc tgaaggtcgt cgttaatcac aaagcgggtc gcccttgtat
    10021 ctgtttgaaa gcctgaatca actgattcaa acctacctgc cggaagacca aatcaagcgt
    10081 ctgcggcagg cgtatctcgt tgcacgtgat gctcacgagg ggcaaacacg ttcaagcggt
    10141 gaaccctaca tcacgcaccc ggtagcggtt gcctgcattc tggccgagat gaaactcgac
    10201 tatgaaacgc tgatggcggc gctgctgcat gacgtgattg aagatacacc cgccacctac
    10261 caggatatgg aacagctttt tggtaaaagc gtcgccgagc tggtagaggg ggtatcgaaa
    10321 cttgataaac tcaagttccg cgataagaaa gaggcgcagg ccgaaaactt tcgcaagatg
    10381 attatggcga tggtgcagga tatccgcgtc atcctcatca aacttgccga ccgtacccac
    10441 aacatgcgca cgctgggctc acttcgcccg gacaaacgtc gccgcatcgc ccgtgaaact
    10501 ctcgaaattt acagcccgct ggcgcaccgt ttaggtatcc accacattaa aaccgaactc
    10561 gaagagctgg gttttgaggc gctgtatccc aaccgttatc gcgtaatcaa agaagtggtg
    10621 aaagccgcgc gcggcaaccg taaagagatg atccagaaga ttctttctga aatcgaaggg
    10681 cgtttgcagg aagcgggaat accgtgccgc gtcagtggtc gcgaaaagca tctttattcg
    10741 atttactgca aaatggtgct caaagagcag cgttttcact caatcatgga catctacgct
    10801 ttccgcgtga tcgtcaatga ttccgacacc tgttatcgcg tgctcgggca aatgcatagc
    10861 ctgtacaagc cgcgtccggg tcgcgtgaaa gactacatcg ccattccaaa agcgaacggc
    10921 tatcagtctt tgcacacctc gatgatcggc ccgcacggtg tgccggttga ggtccagatc
    10981 cgtactgaag atatggatca gatggcggag atgggtgttg ccgcgcactg ggcttataaa
    11041 gagcacggcg aaaccagcac caccgcgcaa atccgcgccc agcgctggat gcaaagcctg
    11101 ctggagctgc aacagagcgc cggtagttcg tttgaattta tcgagagcgt taaatccgat
    11161 ctcttcccgg atgagattta cgttttcaca ccggaagggc gaattgtcga gctgcctgcc
    11221 ggtgcaacgc ccgtcgactt cgcttatgca gtgcataccg atatcggtca tgcctgcgtg
    11281 ggcgcacgcg ttgaccgcca gccttacccg ctgtcgcagc cgcttaccag cggtcaaacc
    11341 gttgaaatca ttaccgcacc gggcgctcgc ccgaatgccg cgtggctgaa ctttgtcgtt
    11401 agctcaaaag cgcgcgccaa aattcgtcag ttgctgaaaa acctcaagcg tgatgattct
    11461 gtaagcctgg gccgtcgtct gctcaaccat gctttgggtg gtagccgtaa gctgaatgaa
    11521 atcccgcagg aaaatattca gcgcgagctg gatcgcatga agctggcaac gcttgacgat
    11581 ctgctggcag aaatcggact tggtaacgca atgagcgtgg tggtcgcgaa aaatctgcaa
    11641 catggggacg cctccattcc accggcaacc caaagccacg gacatctgcc cattaaaggt
    11701 gccgatggcg tgctgatcac ctttgcgaaa tgctgccgcc ctattcctgg cgacccgatt
    11761 atcgcccacg tcagccccgg taaaggtctg gtgatccacc atgaatcctg ccgtaatatc
    11821 cgtggctacc agaaagagcc agagaagttt atggctgtgg aatgggataa agagacggcg
    11881 caggagttca tcaccgaaat caaggtggag atgttcaatc atcagggcgc gctggcaaac
    11941 ctgacggcgg caattaacac cacgacttcg aatattcaaa gtttgaatac ggaagagaaa
    12001 gatggtcgcg tctacagcgc ctttattcgt ctgaccgccc gtgaccgtgt gcatctggcg
    12061 aatatcatgc gcaaaatccg cgtgatgcca gacgtgatta aagtcacccg aaaccgaaat
    12121 taatgtttta tgaacccaac acgttatgca cgcatctgcg aaatgctcgc caggcggcag
    12181 cctgatctga ccgtctgcat ggagcaggtc cacaaacctc ataacgtttc tgcgattatt
    12241 cgtaccgcag atgccgttgg cgtacatgaa gttcacgccg tctggcctgg tagccgcatg
    12301 cgcaccatgg cttcggcagc ggcgggtagt aacagctggg tacaggtgaa aacacaccgc
    12361 accattggcg atgccgtcgc tcatctgaaa ggccagggta tgcagattct ggcaacccat
    12421 ctttctgata acgctgtcga tttccgcgaa attgattaca ctcgcccgac ctgcattttg
    12481 atgggacagg agaaaacggg catcacgcag gaagcattgg ccctggcgga tcaggacatc
    12541 atcattccga tgatcggcat ggtgcagtcg ctgaatgttt ccgttgcctc agccctcatt
    12601 ctttacgaag cccagcgtca gcggcaaaat gcaggcatgt acctgcgtga aaacagcatg
    12661 ttgccggaag cagagcaaca acgcctgttg tttgaaggcg gctatccggt gctggcgaaa
    12721 gtcgcaaaac gcaaaggcct gccttatccc cacgtcaatc agcaaggcga gatcgaagct
    12781 gatgccgact ggtgggctac tatgcaggct gcagggtaag tgccatgaaa ggtcgcctgt
    12841 tagatgctgt cccactcagt tccctaacgg gcgttggcgc agcacttagt aacaaactgg
    12901 cgaaaatcaa cctgcatacc gtgcaggatc tactcttaca ccttcccctg cgctacgaag
    12961 atcgcaccca tctctacccc atcggagaac tactgccggg cgtttatgcc acggtggaag
    13021 gcgaagtgct gaactgcaat atctccttcg gcggtcggcg gatgatgacc tgccagatca
    13081 gcgacggttc cggcatcctc accatgcgct ttttcaattt cagcgcggca atgaaaaata
    13141 gcctggcgac gggccgccgt gtactggctt atggcgaagc aaagcgcggt aaatatggtg
    13201 cggagatgat ccacccggaa taccgcgtgc agggcgatct cagcacgcca gaattacagg
    13261 aaacgctcac gccggtttat ccaacaacgg aaggcgtaaa gcaggccacg ctgcgtaaat
    13321 taaccgacca ggcgctggat ctgctcgaca cctgcgccat tgaagaactc ctgccgccgg
    13381 aactgtcaca aggaatgatg acgctaccgg aagcgttgcg cactttgcac cgcccgccac
    13441 cgacgctaca gcttagcgat ctggaaaccg ggcagcatcc ggcgcaacgt cgtctgattc
    13501 tggaagaact gctggcgcac aacctcagca tgttagcctt acgtgccgga gcacagcgtt
    13561 ttcatgccca gccgctgagc gccaatgatg cgctgaaaaa taaactcctc gcagccttac
    13621 cgttcaagcc aacgggcgca caggcacgcg tagtggcgga gatcgagcgc gatatggcgc
    13681 tggatgtgcc gatgatgcgc ctggtacagg gcgatgtagg ttccggtaaa acgctggtcg
    13741 ccgccctcgc cgcgctgcgt gcgattgccc acggcaaaca ggtggcattg atggcaccaa
    13801 ccgaattact cgccgaacag cacgccaata acttccgcaa ctggtttgaa ccgctcggta
    13861 tcgaagtggg ctggctcgcc ggtaagcaga aaggcaaagc gcgactgtct cagcaggaag
    13921 ccatcgccag cggtcaggtg cagatgattg ttggtacgca cgccatcttc caggaacagg
    13981 tgcagtttaa cggtctggcg ctggtgatta tcgacgaaca acatcgcttt ggcgtgcatc
    14041 agcgtctggc gttatgggag aaaggccagc agcaaggctt ccatccgcat cagttgatca
    14101 tgaccgccac gccgatcccc cgcacgctgg caatgacggc gtatgccgat ctcgatacct
    14161 cggtgataga tgaactgccg ccaggccgca cgccagtgac tacggtcgct attcctgata
    14221 ctcgccgtac cgacatcatt gaccgcgtgc gccacgcctg catgactgaa ggtcgtcagg
    14281 cgtactgggt ttgtacgttg attgaagagt cagagttact ggaagcgcag gcggcggaag
    14341 ctacctggga agagttgaaa ctggcgctac cagaattgaa tgttggcctg gtacacgggc
    14401 ggatgaaacc agccgagaaa caggcggtga tggcgtcgtt taaacaaggt gagctacatc
    14461 tgctggttgc cactaccgtt attgaagtcg gtgttgatgt gcctaacgcc agcctgatga
    14521 ttatcgaaaa ccctgagcgt ctgggtctgg cgcagttaca ccagctgcgc gggcgcgtag
    14581 gtcgtggcgc ggtggcttct cactgcgtgc tactctacaa aacgccgctt tctaaaacgg
    14641 cgcaaattcg cctgcaagtg ctgcgcgaca gtaacgacgg ttttgtgatt gcgcaaaaag
    14701 atctggagat tcgcggccct ggcgaattgt taggcacgcg tcagacgggt aatgctgaat
    14761 ttaaagtggc ggatttactg cgcgatcagg cgatgatccc ggaagttcag cgcctggcac
    14821 gccatattca cgaacgttac ccacaacagg caaaagccct gatagaacgc tggatgccgg
    14881 agacggaacg ttactcgaat gcgtaaaagg cggcagtgag aagaccgcca tttcaggtta
    14941 ccctaccttc ctgccggatg cgattcatca ccctacaaat tcaataaatt atgaatcaat
    15001 acgcaggctt gataagcgaa gcgtatcagg catttgtctt tgttgcggcg gcacttctac
    15061 cgccgcttca tcggttaacc ggcaaaaatc ggcaacatca aatacaactt aattaccagc
    15121 gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa cgccatgtgc
    15181 gacgggccaa agcgttcagt aatcgcctgc atgttggcga ttgccgttgg cgttgcaccg
    15241 aggccaaaac cacagtgacc tgcagccagc actgccgcat cgtagttttt gcccatcatg
    15301 cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac cagaatcgcc
    15361 agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa cgccatcgcc
    15421 aggaacaacg acaagcttac gttacccagc acggataccg cacgctcaaa gacgcggtaa
    15481 aagcccatta ttgacagacc gttgctcaga atcacgccaa caaacagtac acagacgaag
    15541 gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc caccgtcagg
    15601 cagatagcaa tcagcgcgat agtttcaatc agcaccaacg aggtgatcat gcgtcccaca
    15661 tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt cggcgtggtg
    15721 gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag caccagaccg
    15781 aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg ttcaatgaac
    15841 aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga accggccaac
    15901 agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat ggcattttgc
    15961 atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc accggcacgc
    16021 aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat taacggatcg
    16081 cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac tagtagcgcc
    16141 agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt caaaaaggag
    16201 acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac aagcgttgct
    16261 aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt catactcgtc
    16321 atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt atgctttcct
    16381 gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga aaaataccaa
    16441 aagttatata aaaaagtgca tttatctcat ttaaatattt agatgaggat gtggcaaacg
    16501 tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga ttgttttcga
    16561 tgtctgtttc caccctcgag tcagaaaatg cgcaaccggt tgcgcagact caaaacagcg
    16621 aactgattta ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt gccgcctgtc
    16681 agcatctgct ggcgatgttc gttgcggtga tcacgccagc gctattaatc tgccaggcgc
    16741 tgggtttacc ggcacaagac acgcaacaca ttattagtat gtcgctgttt gcctccggtg
    16801 tggcatcgat tattcaaatt aaggcctggg gtccggttgg ctccgggctg ttgtctattc
    16861 agggcaccag cttcaacttt gttgccccgc tgattatggg cggtaccacg ctgaaaaccg
    16921 gtggtgctga tgttcctacc atgatggcgg ctttgttcgg cacgttgatg ctggcaagtt
    16981 gcaccgagat ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt acgccgctgg
    17041 tttctggcgt tgtggtgatg attatcggcc tgtcgctaat tcaggtgggg ctgacctcca
    17101 ttggcggcgg ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa aatctgctgc
    17161 tggcaggcgt ggttttagcc ttaattatcc tgcttaaccg tcaacgtaac ccttacttac
    17221 gcgtggcctc actggtaatt gcgatggcgg ccggatatgc gctggcgtgg tttatgggca
    17281 tgttgccaga aagcaacgaa ccgatgacgc aagaactgat tatggtgcca acgccgctct
    17341 attacggtct tggcattgaa tggagtctgc tgctgccgct gatgctggtc tttatgatca
    17401 cttcgctgga aaccattggc gatatcacgg cgacctctga cgtttccgaa cagccagtgt
    17461 ccggtccgct gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc ctgaactcgt
    17521 ttgtttcggc ggtgtttaac accttcccga actcctgctt cgggcaaaac aacggagtga
    17581 tccagttgac cggtgttgcc agccgctatg tcggttttgt cgtcgcgctg atgttgatcg
    17641 tgctgggtct gttcccggca gtgagcggtt ttgtacaaca cattccagaa ccggttctgg
    17701 gcggcgcaac gcttgtaatg tttggcacca tcgccgcctc cggtgtgcgt atcgtttctc
    17761 gtgagccgct gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg gttggtctgg
    17821 gcgtgtctca gcagccgctg attttgcagt ttgcccctga atggctgaaa aacctgctct
    17881 cctccgggat cgccgcgggc ggtattactg ccatcgtgct gaatctgatt ttcccaccag
    17941 aaaaacagta attcactggc ggcggtaaga taattaccgc cgcagtctca cctctttaac
    18001 cattcccgcc ttgaggattg cgcgtaaatc gtgcataact cccttatgtg catttcacgg
    18061 gatggaagac catgaaattt attgggaagc tgcttctcta cattctcatc gctctgttag
    18121 tggcgatcgc tggcctctat tttcttctgc aaacccgctg gggagcagaa catatcagcg
    18181 catgggtttc cgagaatagc gactatcatc tggccttcgg ggcgatggtt caccgttttt
    18241 ccgcgccatc tcatatcgtg ctggagaatg tcacgtttgg tcgtgatggc cagcccgcga
    18301 ccctggtggc caaaagtgtc gacattgcgc taagtagtcg gcaactgacc gaaccacgcc
    18361 atgtcgatac catcctgctg gaaaacggga cgctgaatct caccgaccag accgcgccgc
    18421 taccgttcaa agccgatcgt ctgcaactgc gtgatatggc gtttaatagc ccgaatagcg
    18481 aatggaaact gagcgcgcag cgggtaaatg gcggcgtggt tccgtggtca ccagaagccg
    18541 gtaaagtgct gggtacgaag gcgcagattc agtttagtgc cggatcgctt tcgctcaatg
    18601 atgttcctgc caccaatgta ctgattgaag gcagtattga taacgatcgc gttacgctga
    18661 ctaacctggg tgccgacatc gcgcgcggga cattaaccgg aaacgcgcag cgtaacgccg
    18721 acggcagctg gcaagtggaa aacctgcgca tggcggatat ccgtctacaa agcgaaaaat
    18781 cgctaaccga cttctttgcg ccattacgct ctgtcccgtc gttgcagatt ggtcgcctgg
    18841 aagtgatcga tgctcgtttg caaggtccgg actgggcggt gaccgacctc gatctcagct
    18901 tgcgcaacat gaccttcagt aaagatgact ggcagacaca agaaggcaaa ctgtcgatga
    18961 acgctagcga gttcatttat ggttcgctgc atttatttga cccgattata aacgcggaat
    19021 tttctccgca gggcgtagcg ctgcgccagt tcaccagccg ctgggaaggg ggtatggtca
    19081 gaacgtcagg gaactggctg cgtgacggga aaacgttgat ccttgatgat gcggcaattg
    19141 ccggactgga atataccttg ccgaaaaact ggcaacagtt gtggatggaa acgacacccg
    19201 gttggttaaa cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg atcattgata
    19261 tcgaccctga cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc aacctgacgc
    19321 tggttaccga tcataaatgg ggcgtctgga gtggctcggc gaatctgaat gccgccgccg
    19381 cgacgtttaa tcgtgttgat gtccgtcgtc cgtcgctggc gctaaccgcc aacagcagca
    19441 cggtgaatat cagcgaactg agtgcattta ctgaaaaagg cattctggaa gccaccgcca
    19501 gtgtttcaca aacgccacaa cgtcagacac atatcagcct gaatggacgc ggtgtgccgg
    19561 tgaatatttt gcaacagtgg ggatggcctg aattaccgtt gactggcgac ggcaatattc
    19621 agcttaccgc cagtggcgat attcaggcca atgtcccgtt gaaacctacg gttagcgggc
    19681 aattgcatgc cgtgaacgcc gcaaagcagc aagtgactca aaccatgaat gcgggcgtcg
    19741 tttccagcgg tgaagttaca tcggcagagc tggtgcagta aacatcaatc tcgccgggta
    19801 cgactcagac gtacccggca ttcttcaata gattacaacg taattgtcag cgcattccct
    19861 tgaggcgtaa ccaccagccc ctgctcactt tcagcctgcg aaccgccttg cagaccattt
    19921 actttcacaa tattgcgcaa gcacagtgtc cagttcctcg cctcgcccgc accagtcaca
    19981 gtaatcgtgt taccagtacg tgttgctttt agagtaaaga tcaccgaacc gtcagcagcg
    20041 ggcacttcac atacagcttc atgcccgtct tgcagattaa agagatggaa tgccgtgcct
    20101 tcgtgccacg cgtaatcggg acgttgatcg ttgttgccca gcgccaatag ggtgttatca
    20161 cgcacataaa cgggcagact caggaagccg tgctgctgtt tatgccagcg actaccgtcg
    20221 agctcattgt tgtgccacag gtgtgtccag cgaccttccg gcaagtagaa ctgcacctcg
    20281 cccgcctcag tgaacaccgg agcaaccatc acgttgtcgc ctagcatgta ttgacggtca
    20341 aggtaatcac aagccggatc gtccgggaac tccatcatca tggcccgcat catcggcgta
    20401 ccccgcgctt tcgcacgcgc agcttcacga tacagatacg gcatcatgcg gcatttcagt
    20461 tgcgtgaaga agcgcaccac atcacaggac tcatcatcgt aggcccacgg cacacgataa
    20521 gatttgctgc catgtaaacg actatggctg gagagcaaac caaacgcgca ccagcgtttg
    20581 taaacgtgtg ccggcgcggt attttcaaag ccgccgatat cgtggctcca gaagccaaaa
    20641 cctgaaaggc caatagacaa accaccgcgc aggctttccg ccattgattc gtagttagcg
    20701 taacaatcgc caccccagtg taccgggaat ttctgcgcac cgacggaggc cgagcgggca
    20761 aacaagacag cttcttcctc accaacggtg tccttgagca cgttccacac cagttcgttg
    20821 tagatgtacg cataatggtt atgcattttc tgcggatcgg aaccgtcaaa ccactgaaca
    20881 tcagttggga tacgttcgcc aaagtcggtc ttaaagcaat caacgcccat cgcgaccaga
    20941 cctttcagtt tgtcggcgta ccatttgcag gcatccggat tggtaaagtc ataaatcgcc
    21001 aga
//
LOCUS       DAJVRF010000066        20711 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJVRF010000066 DAJVRF010000000
VERSION     DAJVRF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20711
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.066"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="OQN13_002229"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="OQN13_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCS1615581.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     EQEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="OQN13_002230"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="OQN13_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCS1615582.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="OQN13_002231"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="OQN13_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCS1615583.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="OQN13_002232"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="OQN13_002232"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCS1615584.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="OQN13_002233"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="OQN13_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCS1615585.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="OQN13_002234"
     CDS             complement(8214..9107)
                     /locus_tag="OQN13_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCS1615586.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="OQN13_002235"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="OQN13_002235"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCS1615587.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="OQN13_002236"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="OQN13_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCS1615588.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /locus_tag="OQN13_002237"
     CDS             complement(10238..11470)
                     /locus_tag="OQN13_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCS1615589.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="OQN13_002238"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="OQN13_002238"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCS1615590.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="OQN13_002239"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="OQN13_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCS1615591.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..16539)
                     /locus_tag="OQN13_002240"
     CDS             complement(14275..16539)
                     /locus_tag="OQN13_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HCS1615592.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASLIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQIAVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="OQN13_002241"
     CDS             complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="OQN13_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCS1615593.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="OQN13_002242"
     CDS             complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="OQN13_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCS1615594.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="OQN13_002243"
     CDS             complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="OQN13_002243"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCS1615595.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPASDALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="OQN13_002244"
     CDS             complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="OQN13_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCS1615596.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4971 a         5732 c         5044 g         4964 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttcctg
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaagcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacacc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgct atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg atagccattg
    15181 ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc agaaaagtaa
    15241 agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg gcccgcttaa
    15301 atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca agggaattgc
    15361 taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga tttgcacggg
    15421 cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg cacgcaatac
    15481 cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca gtgccgcgac
    15541 cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca tcagcaaaat
    15601 tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac ttaacttaag
    15661 cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca ttcccgtcaa
    15721 ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc agtggatcca
    15781 gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca acaccgcaaa
    15841 agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc gcatgatatt
    15901 tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa taaccgcatt
    15961 ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac gcaggctaca
    16021 attcggttca ataagacttg cctgcagaaa gcggccggtg agcggctgat gctgggcaat
    16081 ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg cacgaacttt
    16141 gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt cgccatacag
    16201 cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt gaccgtaatg
    16261 agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg cgccagttaa
    16321 ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga aaaacagcaa
    16381 cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc aggcgaagag
    16441 agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca acggaaaaat
    16501 tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc tgtttatgga
    16561 aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc gaggcacatt
    16621 cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag cttccagttc
    16681 ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca agtttgagta
    16741 aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt aaagtgagga
    16801 acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc ggtacgtggt
    16861 gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc gccctgcgca
    16921 agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt cttggcggga
    16981 atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat gattcctccg
    17041 gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc ggccgtagcc
    17101 gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact caacaaactt
    17161 cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt gaaagcttca
    17221 gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc gattgcatct
    17281 ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt tttacgatca
    17341 acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt agcgtcttca
    17401 acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc agccagttct
    17461 acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc gcctttcttg
    17521 ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc cagggagata
    17581 cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc ttttttgtat
    17641 tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg aaccaggccg
    17701 tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt gattttacct
    17761 tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt agctttgcac
    17821 tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac cataacttcc
    17881 actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt ccaatccatt
    17941 tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca gccgtagtca
    18001 gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt agcgatagct
    18061 acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg ttcgcggtcg
    18121 aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct cggatgctta
    18181 acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc cagatcaacg
    18241 aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc ttcctgcagg
    18301 ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc acgacgagaa
    18361 acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag ctctttgcct
    18421 tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg caggaacgca
    18481 cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat aacaccggta
    18541 acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg acgtttagct
    18601 ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag agcaacgtca
    18661 acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg ctcagccggg
    18721 atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc gatagcaaca
    18781 acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga ctcttcaaag
    18841 agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg tccacctggc
    18901 tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac gggggtactg
    18961 caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc gctttttcaa
    19021 tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggat gccggaacca
    19081 gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg gccaaaaggc
    19141 gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga tgcgcacgtt
    19201 cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc accgttccca
    19261 tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg cgacgcaata
    19321 atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc acttcctgag
    19381 tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg ccattggttg
    19441 acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct gacgcaacat
    19501 caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc gaatccagca
    19561 gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct gcaccgcttg
    19621 ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta aaacaggcgt
    19681 accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta acgttgtgtg
    19741 caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata gcgggctggc
    19801 agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg atcacggatg
    19861 tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt gcgaccgcca
    19921 tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat gccacgtttc
    19981 ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga gtatttagca
    20041 ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga agccacaccg
    20101 ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt catttcagcc
    20161 agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc aatttcgttg
    20221 tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc catcgcccat
    20281 gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt aatattgtta
    20341 cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt acttgcgact
    20401 tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc atcggcatcg
    20461 cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt ggtatcaata
    20521 ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat
    20581 ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta tgtatcaaca
    20641 acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa
    20701 cgccgacaat g
//
LOCUS       DAJVRF010000067        20687 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJVRF010000067 DAJVRF010000000
VERSION     DAJVRF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20687
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.067"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..1132
                     /gene="ypdA"
                     /locus_tag="OQN13_002245"
     CDS             <1..1132
                     /gene="ypdA"
                     /locus_tag="OQN13_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCS1615597.1"
                     /translation="LVQSVEGEKEASAARQTKLALDIANKTLPLFRHVNSESLRKVCE
                     IIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQAMNYGKIIIKNNDEA
                     HRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAVGLSQIISTQLEVSRA
                     EQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTARQLIFNLSRYLRYNIE
                     LKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNCCIPSLLIQPLVENAI
                     VHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERVEANEMPGNKIGLLNV
                     HHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1147..1881
                     /gene="ypdB"
                     /locus_tag="OQN13_002246"
     CDS             1147..1881
                     /gene="ypdB"
                     /locus_tag="OQN13_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCS1615598.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            1894..2751
                     /locus_tag="OQN13_002247"
     CDS             1894..2751
                     /locus_tag="OQN13_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1615599.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(2754..5249)
                     /gene="ptsP"
                     /locus_tag="OQN13_002248"
                     /pseudo
     CDS             complement(2754..5249)
                     /gene="ptsP"
                     /locus_tag="OQN13_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5274..6311)
                     /gene="ypdE"
                     /locus_tag="OQN13_002249"
     CDS             complement(5274..6311)
                     /gene="ypdE"
                     /locus_tag="OQN13_002249"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS1615600.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTAFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6311..7396)
                     /gene="ypdF"
                     /locus_tag="OQN13_002250"
                     /pseudo
     CDS             complement(6311..7396)
                     /gene="ypdF"
                     /locus_tag="OQN13_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7411..8658)
                     /locus_tag="OQN13_002251"
     CDS             complement(7411..8658)
                     /locus_tag="OQN13_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCS1615601.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(8680..9006)
                     /locus_tag="OQN13_002252"
     CDS             complement(8680..9006)
                     /locus_tag="OQN13_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCS1615602.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9226..10191)
                     /gene="glk"
                     /locus_tag="OQN13_002253"
     CDS             complement(9226..10191)
                     /gene="glk"
                     /locus_tag="OQN13_002253"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCS1615603.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            10395..11651
                     /locus_tag="OQN13_002254"
     CDS             10395..11651
                     /locus_tag="OQN13_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCS1615604.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            11766..12092
                     /locus_tag="OQN13_002255"
     CDS             11766..12092
                     /locus_tag="OQN13_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS1615605.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12233..13471)
                     /locus_tag="OQN13_002256"
     CDS             complement(12233..13471)
                     /locus_tag="OQN13_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCS1615606.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            13807..15009
                     /gene="nupC"
                     /locus_tag="OQN13_002257"
     CDS             13807..15009
                     /gene="nupC"
                     /locus_tag="OQN13_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCS1615607.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15059..17247)
                     /locus_tag="OQN13_002258"
                     /pseudo
     CDS             complement(15059..17247)
                     /locus_tag="OQN13_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(17456..17531)
                     /locus_tag="OQN13_002259"
     tRNA            complement(17456..17531)
                     /locus_tag="OQN13_002259"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17496..17498),aa:Ala,seq:ggc)
     gene            complement(17571..17646)
                     /locus_tag="OQN13_002260"
     tRNA            complement(17571..17646)
                     /locus_tag="OQN13_002260"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17611..17613),aa:Ala,seq:ggc)
     gene            17867..18226
                     /locus_tag="OQN13_002261"
     CDS             17867..18226
                     /locus_tag="OQN13_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS1615608.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18228..18620
                     /locus_tag="OQN13_002262"
     CDS             18228..18620
                     /locus_tag="OQN13_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS1615609.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(18672..20087)
                     /gene="gltX"
                     /locus_tag="OQN13_002263"
     CDS             complement(18672..20087)
                     /gene="gltX"
                     /locus_tag="OQN13_002263"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCS1615610.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20346..20421
                     /locus_tag="OQN13_002264"
     tRNA            20346..20421
                     /locus_tag="OQN13_002264"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20379..20381,aa:Val,seq:tac)
     gene            20466..20541
                     /locus_tag="OQN13_002265"
     tRNA            20466..20541
                     /locus_tag="OQN13_002265"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20499..20501,aa:Val,seq:tac)
     gene            20588..20663
                     /locus_tag="OQN13_002266"
     tRNA            20588..20663
                     /locus_tag="OQN13_002266"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20621..20623,aa:Val,seq:tac)
BASE COUNT         5134 a         5518 c         5149 g         4886 t
ORIGIN      
        1 tctggtgcaa agcgttgagg gcgaaaaaga ggccagcgcc gcgcggcaga ccaagctggc
       61 gctggatatc gccaacaaaa cgctaccgct gtttcgccat gtcaatagcg agtcattacg
      121 caaggtctgc gaaattatcc gcgatgacat tcacgccgat gcggtggcga ttactaatac
      181 cgatcatgtg ctggcctatg ttggcgtggg tgaacataac tatcagaatg gcgatgactt
      241 cattagcccg actacccgtc aggcgatgaa ttacggaaaa atcatcatta aaaacaatga
      301 tgaagcccac cgcacaccag agattcattc catgctggtg atcccattgt gggagaaagg
      361 ggtcgtgacc ggaacgctga aaatttacta ctgccacgcg catcagatca cctcgtcatt
      421 acaggaaatg gcggtcggtc tgtcgcaaat catctccacg caactggagg tttcacgcgc
      481 cgagcagcta cgtgaaatgg caaataaggc agagcttcgc gccctgcaaa gcaaaattaa
      541 tccccatttt ctgtttaacg ctctgaacgc tatttcatcg tcaatccgtc tgaatccgga
      601 taccgctcgc cagttgatct ttaatctgtc gcgttatctg cgctataaca ttgaattaaa
      661 agacgatgag caaatcgata tcaaaaaaga gctgtatcaa attaaagact atattgccat
      721 tgagcaggcc cgctttggtg acaagctgac ggttatctat gatattgatg aagaggtgaa
      781 ttgctgcatt cccagcctgc tgatccagcc gttggtggag aacgccattg tccacggtat
      841 tcagccttgc aaaggtaaag gcgttgtcac catcagcgtt gcagagtgcg gaaatcgggt
      901 acgcattgcg gtgcgagata ccgggcacgg catcgatcca aaggtgattg agcgggtcga
      961 agcgaatgaa atgccgggca ataaaattgg cctgctgaat gtccatcatc gcgtgaagtt
     1021 attgtatggc gaggggctgc atatccgccg cctggagccg gggacggaaa ttgcgtttta
     1081 cattcctaac caacgcaccc cagtcgcctc acaggctacg ttattgcttt gagccggagt
     1141 gatattgtga aagtcatcat tgttgaagac gaattcctgg cacaacagga actgagctgg
     1201 ctaattaaag agcacagcca gatggagatt gtcggcacct ttgacgacgg tctggacgtg
     1261 ttgaagtttt tgcagcataa ccgcgtcgac gccatttttc tggatatcaa tattccgtcg
     1321 ctggatggcg tgttgctggc gcaaaacatc agccagttcg cccataaacc gtttattgtg
     1381 ttcatcaccg cgtggaaaga acatgcggta gaagcgtttg aactggaggc gtttgactac
     1441 attctcaaac cgtatcagga gtcacgtatt accgggatgc tgcaaaaact ggaagcggcc
     1501 tggcaacaac agcagaccag cagtacgcct gccgcgacgg taacgcgtga gaatgacacc
     1561 attaatctgg tgaaagatga gcgaataatc gtcacgccaa ttaacgatat ctattacgcc
     1621 gaagcgcacg agaaaatgac ctttgtctat acgcggcgtg aatcctacgt aatgccgatg
     1681 aacattaccg aattttgcag caaactgccg ccgtcgcatt ttttccgctg ccatcgctca
     1741 ttttgtgtca atctgaacaa aatacgcgaa atcgaaccgt ggtttaataa cacctacatt
     1801 ctgcgactga aagatctgga ttttgaagtg ccggtcagcc gcagcaaagt gaaagaattt
     1861 cgccagttaa tgcatcttta atcgggaact ttcatgaaag caccaggctt gcccgccgat
     1921 caacaatttt tcgccgatct gttcagcggc ctggtgctta acccgcaact actggggcgc
     1981 gtctggtttg ccagccagcc tgcctcgtta ccggtgggca gtttatgtat tgattttccc
     2041 cgtctggata tcgtgctgcg cggcgaatac ggcaacctgc tggaagcaaa gcagcagcga
     2101 atggtggaag gagaaatgct gtttattccg gcacgcgcgg ctaatttacc gatcaacaac
     2161 aaaccggtga tgctgttaag cctggtgttc gcaccgacct ggcttgggtt atcgttttac
     2221 gatagccgca ccacgtcgtt gttgcatcct gctcgccaga tccagcttcc cagcctgcaa
     2281 cgcggtgaag gtgaagcgat gcttaccgcc ctcacccatc ttagccgttc gccgctggag
     2341 cagaatatca ttcagccact ggtgttaagt ttgctgcatc tttgccgcaa cgtggtgaat
     2401 atgccgccgg gtaattcgca accgcgcggc gattttctct atcacagcat ttgtaactgg
     2461 gttcaggata attatgccca gccgctcacc cgcgagagcg tggcgcagtt ttttaatatc
     2521 acgcccaatc atctgtcaaa actgtttgct cagcatggaa cgatgcgttt tatcgagtat
     2581 gtgcgttggg tgcgaatggc gaaggcgagg atgattttgc agaaatatca tctgtcaatt
     2641 catgaagtgg cacagcgttg cggttttccg gatagcgact atttttgtcg cgttttccgg
     2701 cgtcagtttg gtctgacgcc gggagagtac agcgcccgtt ttcagggcta acgtcagaag
     2761 gttaattctg tttccagcag cgtcaggata ctttgtgcat cttgcgcggc aaacaacgac
     2821 tggcggaagt ttttattcac cagtttacgc gccagctgcg agaagacttt cacatgattc
     2881 atcccttcgt tagcacccag cgtcagcatg atcaccagtt caacttcgcc catttctgac
     2941 tgccagtcaa ccggtttcgc cagccgggca atgctgatac tggaatgacg gatccactga
     3001 gatttggtgt gcgggatcgc tacgccaaaa ccaacgccgg tggtaacaat ctcttcccgc
     3061 tgccagacat cttcttccag ctcaaacgga tgttcagtgc gcccgttaac gccgaggttg
     3121 ccgcacagga actggatcgc ctgctcttta ttgctaaaat cctgatcaac aaagatattc
     3181 tccagcgcca gcagtgggcg aacgtcttct tccggcgtaa aggcggtgag taacgcttca
     3241 atttcctgcg cactgcggca ttcacatgcc tgacgcgcca gttcccgaca cgcctcgcta
     3301 tccagttgac gaagctggct tttcaccgcc ggaatacgcg ggctactcat actcagctca
     3361 tccaggccca gcccaagcag tagcggcaga taacggcttt caccgcccag ttcaccgcaa
     3421 atgcctaccc atttgccccg ctgatgcgca gtggtaacta tttgctgcaa catgcgcagg
     3481 aacgatggcg taatcgggtt atatagcggc gatacgcgcg ggttattacg atcgaccgca
     3541 tacagatact gggtcatatc gttggagccg atactgaaga aatcgacctc atcgcagaag
     3601 tggtcgatga tgtagcacac cgacggcact tccaccatga tcccaagcgt aatcgtctct
     3661 gcatgacgca ggccatcgcg cttaagctca acgatcgctt tttggatctc gcctttcacc
     3721 cataagatct gatcgaggct gttaaccatc gggatcatca actgggcgtt gccgaaactg
     3781 gcggcgcgca aaatggcccg cagttgagtg cggaacaggc cagcaaattc cgggtaaata
     3841 cgtaccgcgc gatagccgag gaacgggttc tcttcctggg gaatattcag ataaggaatg
     3901 cttttatcgc cgccgatgtc catcgtgcgg aagataatcg gcttgtcgcc cgccgccagt
     3961 agcacctgct ggtaggcttc aaattgctcc tgctcgtccg gcgcgctgtc gcgatccata
     4021 tagagcattt cggtacggaa cagcccgaca ccttccgcgc cgttggcaaa cgcgcctggc
     4081 gcttccagag cggtgccgat attcgccgca atatcaatac gcttgttatc acgggaatag
     4141 gccagctgcg cggcagcctg cgcctgttgt ttttggcgtt tatccgccag cgtctgcgcg
     4201 acctgataat aaccgctcac cgcgtcattc gggttaatcg ccagcacgcc gcactgggcg
     4261 tcaagcactg caggttgccc ggcataacgg gcaatcgcat ccagcggcaa gccactcagt
     4321 accgggatcg ccgaggcacg ggccagaatc agtgtatgcg aggtgcgccc ggttttctcc
     4381 agaatcatgc ccgcaagatt tttcaaatca aggctcaaaa actggcttgg ggttaaatct
     4441 tcagccacca gaatggtcgg tttttccagc accagattgt tgcgcggctt cagttccggc
     4501 taggtgatat gcagcaactg ttcgctgata tcgcgaatgt cgctgacccg ctcgcgcaga
     4561 taatcgctgg cagaagcaga tagtttggcg caaacctgct ccatattgct gatgatcgcc
     4621 gcccccagtc cctgatgctg ttctgtcatc aggcgacgga tattgcctgc aaattcatca
     4681 tcctgaatca gcgacaaatg agcgctgagg atagttttgc tttcgccgtc acgctcacgc
     4741 aattgctggt tcagttgctc ggcaagcgtt gccaggctgt gctccagccg ggtggaatct
     4801 tgcgcactgg cggggattgc ccgataactg tcgaggctgt cgctttgtaa cagggtcagc
     4861 gtacccacgc cgacgccgct tgccagcaca ttgccgtaca gtaaatccgg gttaaggcgg
     4921 cttaatgaac gcggcagcgg atgcgccgtc agttctgcct gcgtcggctg aacgctgtcg
     4981 ctgtcgataa agcgcaccag gatgtactct tccagcacgc gccgcgcctg ctcttcatcg
     5041 ctgccgctaa tgttcaggct gcaactgtca ttaaataagg tgccagttcc aatcaacgcc
     5101 agcgagcttt tagcatcggc ctttgcgttc tggcgatggt tgataaaggt gatttcgctt
     5161 tgccactggc tgcactgttc tttaagttcc cacgccggac gagcatgtag accgttaggc
     5221 aaaggacaga gaaattgaat cgttaacata accgctccgg gttaatcagg agatcatctg
     5281 aaatccgtca gttgaacaac cgtctcacgc gtaagacgtt gaataagggc agataaaagt
     5341 tgctccatct gcaaaatgtc gcggcaatcg gcaatcgatg ccgcgcaatg tccgtggcgg
     5401 gtggcaggcc ccattaccac tgtgggtacg ccagtatcgg ttaagtgcac cgctccaccg
     5461 tccgtgccgc cgttactgaa catatccgcc tgcaacggaa cgccaatttc tgccgacacg
     5521 gtttcaatcc acgcggtaag ttttggcggc gcaatcagcg atttatcgct taacaccagc
     5581 atcggcccgt taccaatctg acgatggtta gccgcgccat aatcaaagtt tttcgcccag
     5641 caggcggtat caagcacaat ggcgacgtcc ggcgacaccg cgcgggtagc agtttgcccg
     5701 ccgcgtaatc ccacctcttc gctggaactt gccaccagcc acacttccgc aggcagttcg
     5761 gcgtcgtgca gctcacgtag taacgtcacc agcagatagc aaccgaggcg gtcatcaaag
     5821 gctttcccca tcacccgctg gtgagggaga acctgaaaag ctgtatcaaa ggtgacgcga
     5881 tcgcctggac gaattcccgc ctgcatcact tcgtcatacg agcgcgcacc aatgtccacg
     5941 cgcatggcgc tgacgtcatt cccctgccgg tcgccgtcaa gcaggcctgg aattttgcac
     6001 tcttcacggg tggtgatgcg caccggctgc agctggcggg cagccatgcg cacgttgcca
     6061 accggcagca catcaattgc cccttcgcgg gagatgctgc gcaccataaa tcccacttcg
     6121 tccatatgcg cacagatcat caccttcgga cctgtcgatt cattgaggcg gatcagcacc
     6181 gatcccagac catcaaatcg tacttctttt tgcaggcgat ccgcttcttc cagtaggatc
     6241 tgccgcactt cctgttccga ggaggcgatc gcatctgcct cgctcaacgc ttttaatagc
     6301 gataaatcca ttatgcctct cccgtgagca acactgtttt cggcatggcg tagagcactt
     6361 ctgcgccttg cggggtaacc agcacaacat cttcgatgcg cacgccccct tgccctggca
     6421 aataaatccc cggctccacg gtcagtaaca tgcctggctg tagcgtcgtg gtatcccgcg
     6481 gtgaaaagcg tggatcttcg tgtacttcaa taccgatagc atgaccggtg ttgtgaccaa
     6541 aatagtcgcc aaatcctgcc tcggtaatca cccggcgcgc ggcgtcgtca acctgttagc
     6601 agcgtacgcc agggcgaatc gcggagattg ccgccagctg tgcttgcagg acaatctgat
     6661 agacgttaaa cagcgggtga gattcggcaa ttaccccttc gccattcacc agcaaggtgc
     6721 gcgtcatatc agagcagtac ccctgataga gcgcaccaaa atcaagagtg acaaactcgc
     6781 ctgctgcaac aatcttgtcg ctggctttgc cgtgcggcag cgccccacgc cagccgctgg
     6841 cgacaatggt gtcaaaagag gctttttctg cgccctgctg gcgcataaac cactccagtt
     6901 cagcggctat ctcgcgctcg ctcatccccg cctgaataaa gcggcgaata tgctccgctc
     6961 cgcgatcggc aatcccacag gcgaggcgga ttatctccac ctcctctggc gttttgattt
     7021 gccgcagcac atccggcgtg gcgctaacca gtttcgcatt gagttcagac ttccagcggt
     7081 gcgcggtttc ccaactcacc tgctggcctt caaaaccgag cgtttgcaac tgctcatcgg
     7141 caatgatttg attgacgata gtggtaagcg tgttcgtcgc gtcaagcaaa tgcagctggt
     7201 agccttgcgc acgggcttcc acctcaacga aatagcgcga atctaccaga atgtgcgcac
     7261 tttcacggct aataaccaca tagcctgatc cggtggagat ccccagatgc ggctgtttgt
     7321 tctgccgtga ggagagaagc actgcatcca gttgttgcgc cttaagccag tcgcgcagcg
     7381 aagcgagtaa tgtcatctgg attttccttc ttacaggcta tcgattaaca atttaccttt
     7441 acggaacatc atcagacgca ggaacaccac catcagcgca gttatgactg cccccagcgc
     7501 gatccccgcc atatagacgc ccaggttagt gaccagcggc catgcccaga tagcggattc
     7561 cgggaaccat tgcactgcgc ccagccagac agcggcggtt gagccgacaa tcgcgccgac
     7621 catatacgac ggaatggcgg tgatggggct ttccagcgca aacggaatcg caccctcact
     7681 aatccccata aaggcaagga acatcgcggt cttaccctgc ggataaagtt gagcgttgaa
     7741 caggcgtttg ccggttaaac gacggtcgat aatggtcgcc agacccagac ctatcggcgg
     7801 aataacgata gcgatagaac gtgcagtgac cggtaatacg tggtcagtgg taaagctgaa
     7861 ggcaacaaaa cctgcggctt tgttgatcgg gccaccaagg tcgattgcgg tcgccgcggc
     7921 gatccccatt gcgtacatca gcgcgccctt ctcacctgcg gcagtcagca cggtacggat
     7981 accgccgttg atccagccgc caaagggggt aatgacgtag tacatcgcca gcatgacaaa
     8041 aatggcggaa agaatcggta gcaaaaatgt ggttttgaac gctaacagga aatccggtaa
     8101 ctggattttc tggttcatcc acttcacaag gtaacccgcg acgatagaaa taatcagcgc
     8161 accaatgaag gtggacggca ccggcgaaga ggtcgcccat tgcatagtgc tgggatcgaa
     8221 gttcagcagt tgcgtcggct gggtagacat caagccgccg ataaaaccag ccgggaacgc
     8281 cagtttgcca ccgatagagt tcgccacaaa agcagcaaac atcggaatgg caaaaccaaa
     8341 caacacgccg ccgaaggact gcgacagcca ggcaaatttc agtaaggaga ggtcgaaacc
     8401 ggagaatttc ccgctattaa gagcatccat gatgccgatc tcagcaggaa ttttaagcca
     8461 gctataagca atcagctggc tgaaagcgag gatcacaccg cccataatta aggtcggcac
     8521 catgcgtgaa atccctgaca tcacatgctg cgggagttca ccccagaagc tgcttttaga
     8581 ggcctgcgga ttcttaactg ccgctgccgc accggatgcg ccaggcacaa cggttgcact
     8641 gcgtttttta atggccataa caattatccc tgtcgaatat tactgttgtt cagaggcaat
     8701 catctcttcg atttctttga tgatgcccgc agcgttttta attgcgtcct gcaaagtgat
     8761 ttcataaacg tcgcgtgatt cgaaacgctc attatcttcc ggggtaactg ccacggagtg
     8821 gatgatgatg gttgcttcgg cgatatcctg cgccgtcagg cgattctgga taccgtccgc
     8881 gccctgggtt tcaattttca cttcataacc tgcttctacc gccgcttctt ccagcgcctg
     8941 agcggccata aaggtgtgag ccaggcccat tgggcaggca cataaggcaa ttagtttctt
     9001 actcatcgtt aattcctcgt tcagtgatag ttgacgagtg aaattatgaa cgtgcaaaac
     9061 aaatcgccgt taccagacaa ataatgcatt tactggagaa ttaatcccgc tgtacggaag
     9121 tgtgacgcag gtcggccttg tgagggggac acaaagaaaa attgaattaa ctgtatgatt
     9181 taaaagatta tcgggagagt tacctcccga tataacagaa aggatttaca gaatgtgccc
     9241 taaggtctgg cgtaaatgtg caccggaacc gagaagaccc ggattgtcat ggacgatgag
     9301 atacaccgga atatcatgga catattcttt aaagcgccct ttatcttcaa atgcggcacg
     9361 gaaaccagag gctttgaaga actcaaggaa gcgcggcacg ataccgccag caatataaac
     9421 cccaccaaag gttcccagcg ttaatgccag attgccgcca aaacggccca taatgacgca
     9481 aaacagcgac aacgcacggc ggcaatcggt gcagctgtca gccagcgcgc gttcggtaat
     9541 atcttttggc ttgagatttt ctggcaggcg gttgtcggct ttaacaattg cgcgatacaa
     9601 attcaccagc ccaggaccag aaagcacgcg ttccgccgaa acatgaccaa tttccgcacg
     9661 caatatttcg aggataatgg cctcttcttc actattcggc gcaaaatcaa cgtgaccgcc
     9721 ttcgcccggc aagcttaccc aacgcttatc gacatggacc agatgcgcaa ccccaagccc
     9781 tgttccggca ccgtaaaccg caataggttt accttcgacc ggttctgcgc caccaaactg
     9841 aatcagatgc tcttttttca gcatcgggat cgccatcgat acagcggtaa aatcgttaat
     9901 aatttccaga tggctaaaac cgagattctt tttcatttcg gcaattgaga acgcccaggt
     9961 atggttggtc attgccaccc agtcaccggt aattgggcaa gcgatggcaa tacagccgtc
    10021 tttcacctcg accttatgtt cttcaagata aacgcgaatg accgcttcga ggctggggta
    10081 atcaagccct gaataggtct tagcctgcga gatttcacca ctggcaatat cacacagagc
    10141 aagacgtgcg ttggtgccgc ccacatcacc gactaatgca tactttgtca ttcttcaact
    10201 gctccgctaa agtcaaaata attctttctc acactgtaaa tacctggggg cataacaaca
    10261 acgccggaaa ggcaggctcc ctgtaaatat cgatctgggt cacacattta ctttatcgtt
    10321 tcagcaccaa ttgcagcgat gcctttttgc aagctgggca aactaagtat ctgaccccgc
    10381 ataaggaata gaacatgctc catccgcgag ccagaaccat gttgttatta tcgctccccg
    10441 ccgtggcaat tgggattgcg tccagtctta ttctgattgt ggtgatgaaa atcgcctcgg
    10501 tattacagaa tttgctctgg caacgactgc cgggaactct ggggatagcc caggattcac
    10561 ctatctggat catcggtgta ttaacgctaa cgggtattgc ggtggggttg gttatccgtt
    10621 tcagccaggg tcatgccgga ccagaccccg cctgtgaacc gctgatcggc gcaccagttc
    10681 cgccctctgc gctaccagga cttatcgtag cattaattct cggtcttgct ggcggcgtca
    10741 gcctggggcc ggaacatccg atcatgaccg tcaatatcgc ccttgccgtc gccattggcg
    10801 ctcgtctgtt accgcgcgtc aaccgaatgg agtggactat tttagcctct gccggaacca
    10861 tcggcgcgct gtttggtaca cctgttgcgg cggcgttgat attttcgcaa accttaaatg
    10921 gcagtagtga agttccgcta tgggatcgtc tttttgcgcc gttaatggcg gcagcagctg
    10981 gtgcacttac taccggatta tttttccatc cccatttttc actgcccatt gcgcattacg
    11041 gacagatgga aatgaccgat attctcagcg gtgcaattgt cgcggcgatt gccatcgcag
    11101 cagggatggt cgccgtatgg tgcttaccac ggttgcacgc gatgatgaat caaatgaaaa
    11161 atccggtgct cgtgctgggt attggcggat ttattctcgg aattctgggg gttattggtg
    11221 gaccagtttc gctgtttaaa gggctggatg agatgcagca gatggtggca aatcaggctt
    11281 tcagcaccag cgattacttt ttgctggcgg taattaaact tgccgccctg gtcgttgctg
    11341 ccgccagtgg ctttcgcggt gggcgaatct tcccggcagt gtttgtcggc gtggcattag
    11401 ggttgatgct gcatgagcac gttcccgccg ttccagcggc aataaccgtt tcctgcgcta
    11461 ttctcggcat cgtgctggtg gtaacacgcg atggctggtt aagtcttttt atggcggcag
    11521 tcgttgtacc caataccaca ttgctaccgc tgctctgtat cgtcatgctt ccggcatggc
    11581 tgttattagc aggtaagccg atgatgatgg tcaatcgtcc gaagcaacag ccaccccacg
    11641 ataatgttta gcaaaaaatg ctctccttta tgatttaaga gttatggcga tgatacgggg
    11701 ctttacctcc ccgtaatatt gcgttaacag gccgctgacg gcttcgatcg tgaaggagaa
    11761 taacaatgtt caggtcactg tttctggcgg ccgccctgat ggcatttacc ccgcttgcag
    11821 caaatgcagg tgaaatcacc ctactgccat cgatcaaatt acaaattggc gatcgcgatc
    11881 attacggtaa ttactgggac ggtggtcact ggcgagatcg tgattactgg catcgcaatt
    11941 atgaatggcg caaaaaccgc tggtggcgtc atgataatgg ctatcatcgt ggctgggata
    12001 agcgtaaagc atatgagcgt ggctatcgtg aaggctggcg cgatcgtgac gatcatcgcg
    12061 gaaaaggccg cggacatggg caccgccatt aaacgcgtcc tccaatggag cacaatgcct
    12121 gatgcgacgc tagcgcgtct tatcaggcct acaaaccggc tctttaacgc gtagggcgga
    12181 taaggcgttt acgccgcatc cgccaatagt gccagatgcg acgctcattc aactacaatc
    12241 ccagcgccgt ccccaccaac aaccagatat tcagcgccac gaccagcact acaatcaccc
    12301 agcctgtctg ttttacgcgt ttgctgttca ccagatcgcc catcaacttg ctgtcactgg
    12361 tgaaaatcag cagtggaacc agcgccaggg cgataccaaa acttaacagc acctgactca
    12421 taaccagaat ccgtgtcgga tctaatccca tcagaatgac aataaatgac ggcaacatgg
    12481 tgactgtacg acgcacccac agcgggatat ggaagcgaat gaatccctgc atcaccacct
    12541 gccccgccag tgtccccacc accgttgagg acagtccggc agcaaccaga cttaacccaa
    12601 agaccgttgc cgcagcatgg cttaacagcg gttgcagcgt cagataagcc tcatcaagat
    12661 cggcaacacc agtatgacca gaaaagtgga acgccgccgc agctgtagcc atcatcgcca
    12721 gattgacaaa accggcaatc gtcatggcga tagccacatc ccatttggtg gcggaataac
    12781 gttgttgacg cgaaccgcca tgtaaatgct gagtgagcga agagtgcaaa taaatcacat
    12841 gcggcataat cgtcgcccct aacacgcctg ctgccaggaa gaccgcctcc gaagtaggta
    12901 aactcgggat caccattcct ttacccagct gcgccaggtt aggctgggag aaaatcaact
    12961 cgacaatgta agccgcggca acaaacaaca gtaacccgcc aatcactttc tccagtggtt
    13021 tttgcccgcg acgttgcagc attaaaatca ggaaagtcgc gatccccgtc agcaccgcgc
    13081 cctgcaacaa cgagacacca agaatgagtt taaaaccgat cgccgcacca ataaattccg
    13141 ccaggtcggt tgccatcgca ataatttctg cctgaaccca atagaaccac actacgggac
    13201 gcggatagtg atcacgaatc tgctccgcca gattttttcc ggtggcaatc cctagtttgg
    13261 cagagaggat ctgaatcagc atcgccatca ggttggccca aacgacaacc cacagtagct
    13321 gatagccgaa gctggcaccc gcctgaatat tggtcgcaaa gttaccggga tcgatataac
    13381 caatcgccgc aatgaacgca ggtcccatta atgcgagcct catcttgcgc gccgcccgtc
    13441 cgctgctact ctcaacgcga tagttcgtca tcttgtgcct ctaaaacata gcctttgcta
    13501 tgtttcatgc tatgataaac gagaatgatt atcaaattca tttaaatgga ttgtggtgat
    13561 ttctctgata gaccaggatt atgactacga aaagattgat gacttgaatg ttatggttat
    13621 gtttaatgtt agcacattta cataactttc agcttccata cacaacatag cagaaatgta
    13681 tgacagatca ctatttttga agcctgtcac aggacgtcat tatagtgtgt gtcagatctc
    13741 gttttcctta accatgttac atagaatgtg cacggaaatt taacctgcct catatttgga
    13801 gcaaatatgg accgcgtcct tcattttgta ctggcacttg ccgttgttgc gattctcgca
    13861 ctgctggtaa gcagcgaccg caaaaaaatt cgtatccgtt atgttattca actgcttgtt
    13921 atcgaagtgt tactggcgtg gttcttcctg aactccgacg ttggtttagg cttcgtgaaa
    13981 ggcttctccg aaatgttcga aaaactgctc agatttgcca acgaagggac taacttcgtc
    14041 tttggtagca tgaatgatca aggcctggca ttcttcttcc tgaaagtgct gtgcccaatc
    14101 gtctttatct ctgcactgat cggtattctc cagcacattc gcgtgttgcc ggtgattatc
    14161 cgcgcaattg gtttcctgct ctccaaagtc aacggtatgg gcaaactgga atcctttaac
    14221 gccgtcagct ccctgattct gggtcagtct gaaaacttta ttgcctataa agatatcctc
    14281 ggcaaaatct cccgtaatcg tatgtacacc atggctgcca cggcaatgtc caccgtgtcg
    14341 atgtccatcg ttggtgcata catgaccatg ctggaaccga aatacgtcgt tgctgcgctg
    14401 gtactgaaca tgttcagcac ctttatcgtg ctgtcgctga tcaatcctta ccgtgttgat
    14461 gccagtgaag aaaacatcca gatgtccaac ctgcacgaag gtcagagctt cttcgaaatg
    14521 ctgggtgaat acattctggc aggtttcaaa gttgccatta tcgttgccgc gatgctgatc
    14581 ggctttatcg ccctgatcgc cgcgctgaac gcactgtttg ccaccgttac tggctggttt
    14641 ggctacagca tctccttcca gggcatcctg ggctacatct tctatccgat tgcatgggtg
    14701 atgggtgttc cttccagtga agcactgcaa gtgggcagta tcatggcgac caaactggtt
    14761 tccaacgagt tcgttgcgat gatggatctg cagaaaattg cttccacgct ctctccgcgt
    14821 gctgaaggca tcatctctgt gttcctggtt tccttcgcta acttctcttc aatcgggatt
    14881 atcgcaggtg cagttaaagg cctgaatgaa gagcaaggta acgtggtttc tcgcttcggt
    14941 ctgaaactgg tttacggctc taccctggtg agtgtgctgt ctgcgtcaat cgcagcactg
    15001 gtgctgtaaa accatacata aaaaagccgg ggatcattcc ccggcttttt tacgcctgtt
    15061 aatcagctaa tggctgcggg cgaccaatca aatacccttg cagatattgc accccgagct
    15121 tatgcaatag cgcctgctgc tgttgcgtct cgacaaactc cgcgaccaca ctcaatgact
    15181 tcgctttcgc cagatcggta attgatctca caatcatcgc atccagcgta ttcgtgacaa
    15241 tatctttcac aaagacgcca tcaattttga tgatatcagc ctgcaaacgc tttaaccgtt
    15301 cgtagttggc atatccggtg ccaaagtcat caatcgcaat ccggaaacca aacttatgca
    15361 gctgctcgat gttgtacatg ctgctttctg cgttagaaaa cgcctgctcc tcggtgatct
    15421 caagaatgac cgcctgcggg gagatgtgat aacgcttaaa cagacgaata atccgcccgg
    15481 caatattctt ttgcagcagc gtgagcggca ttaaattgac tgaaaagcgc ggtccttttt
    15541 tgtcgcaagg gtgtgttgcc agccacttca acaaggattc cagcacttgc aaatcaaaac
    15601 gcgcgctaag gttaaactga gcaataaggg gcagaaactt atccggggtc ataatgccgc
    15661 cgtcatattt cagtcgcgcg aggatctcat cataaccttc accctctttg ttgcgaattg
    15721 gctgggcgta gagcagcaaa tcaccctgat ctaacgccgt gcgaatggta ttcagcaata
    15781 gcacctgttt agtggtctgc ccggaaacca tctcctctct gctatccagc gccagcacat
    15841 gatgatgtgc gcaggattgc tccgccagcc agcttaactg ccccaacaag ggttgcaggg
    15901 tttcctgatt tccatcaaaa cgcccccagg cagcaccata gcccatatcc agcccggtat
    15961 tgttccagtg aatttgccga ctattcagga tgttaaccat atgctggagt cgcccttccg
    16021 tttccggccc gctcagcact aacagcagtt cactacccgg caattgatat aacttttcgt
    16081 tttcctgcat caacggcagc agcgtacggt aaattgatcg gatacagtga acgcgcatca
    16141 ttaagccgta atgacgactc ataaactcaa gattatcaat gcgcaggcag caaaactctt
    16201 gcccgcctct tgctccggcg cttgttccaa cgcacggaag ttgggcagga gcgttaacgg
    16261 atcggtcagc gcctgcaaat gccagcggcg attaagccat tcactgcgat gataaatgcg
    16321 caccatatag agcaggcaaa cgctaaagga aatcagcacc gcaagaataa atgccagcga
    16381 atattcggtt tccacccctt gcaaaaagtt ctggttgtaa ttcagaaggc aaagcgttga
    16441 aacagccctg gtgagattta aaaacggata gcgaagctta ccgaccccga gggtaaagat
    16501 gatgaagaaa acgggtacca ggtaaccggc aataaagtcg ttttcataag gtgtgcacaa
    16561 cagaagtaga agcacgctta acgctgccag ccagcttaag gtaaatgcgc gtttctcttt
    16621 gcccaacgac ggcgcgatat ccctgcgcca caatatctgc gcaaagtggg gacttacaat
    16681 catgcgagtg agatagtaga aaagcatgtt gtaaataagc acagcggtga aaaggcttag
    16741 caaatcaacg accgtgaaaa tggcatccgc atcgccgaaa aaggtggata tctttagcgg
    16801 gaaatcaaag aagctcccca caagatacat gctgcatttg atgccgatcg gcgtcaccaa
    16861 accaagccag aacagacgtt gccagacata tcggctggtc aatccataac gccaacgcgt
    16921 ccccagctgc cagcgtaata ttgcgcaggc acacaacacg acaaacgtct ggctaccgag
    16981 catgaccgca gtctgtaaaa aagagagatg aaagttccac agattggtgc aaaacatccc
    17041 gcataaaacg ggaacgacac cgcgccagcc aaagataaac aagattgaca gcattacgca
    17101 cagtggcatc cacgccagaa agatataact ggaatggata atggcaagtg gcgaaataaa
    17161 acgggatagc tgaataagta ccacggtgag cgtaaacgct agtgtgaata tcttgatatt
    17221 ttttatcagg ttatgctcca caaacatgag tacactactc tcactgatcg gggttttcag
    17281 gcacacaaat aataagttta ttcgcatcgc gaagcaaaca gggggccagc ctgttgccga
    17341 tttttcatcc aatgatgctt tcaccaggcg gcactaaccc ggacgtagac tgtattacaa
    17401 aagcggcaaa aagcagagac aaaaaacccc cgctttgcag cgagggttgg aaatttggtg
    17461 gagctaagcg ggatcgaacc gctgacctct tgcatgccat gcaagcgctc tcccagctga
    17521 gctatagccc cacgatgcgt ttacgtacca aatttgctgg gtgcaaaatt tggtggagct
    17581 aagcgggatc gaaccgctga cctcttgcat gccatgcaag cgctctccca gctgagctat
    17641 agccccgtca cgtaaagctt gtcgagttga cgggcggcat catatgaatt ccgcccgaat
    17701 gtgtcaacgg caaattgcaa cgtgtagttt caatcgctga aaaatcaggc aaatgaacaa
    17761 ttttggaaaa ccgctcgcat tcagtagtta ttcatgtcac ggtttcctgt aaagtggtgt
    17821 tataaaatga actactaata gacccacata cattcaggga attgttatgt tcaaggagag
    17881 gatgacacca gatgaacttg ccagactgac cggctatagc cgccagacca ttaataaatg
    17941 ggtacgcaag gagggctgga caacgtcacc aaaacctggc gtccagggtg gcaaagcccg
    18001 actggttcac gtcaatgaac aggttcgtga atatattcgc aatgctgaac gtccagaagg
    18061 tcagggagaa gcgcctgccc tttctggtga tgcgccgctt gaagtattgc tggtgacact
    18121 ggcgaaagag atgacgccag ttgaacaaaa acagtttaca tccttgcttc tgcgggaagg
    18181 gattatcgga ttgttacaac gcttagggat tcgcgatagc aaataatatg aaaagattac
    18241 gcaataaaat gaccaccgaa gaactggctg aatgcctcgg tgtggctaaa caaactgtta
    18301 accgttggat cagagaaaaa ggctggaaaa cggaaaaatt tcccggcgtg aaaggcggtc
    18361 gtgccagact tattctggtc gatacgcaag tttgcgagtt tattcagaac acgccagcct
    18421 tccataacac accaatgttg atggaagctg aagagagaat tgctgaatac gctcctggag
    18481 cacgcgcccc ggcgtatcga caaatcatca acgcgataga taatatgaca gacattgagc
    18541 agggaaaagt cgcgcaattt ttgtcacgtg aaggaattcg caatttcctc gcccgtctcg
    18601 atattgacga atcagcataa ataaaaaacg gcaggatatt atctcctgcc gtttatcttt
    18661 ttacacgcta attactgctg attttcgcgt tcagcaataa aatccagcgc tttgttgata
    18721 cgctcgatac tgcgggtctt accaattgcg tgaacggtaa catccagtgc tggagactgc
    18781 cccgccccgg ttacggcgac acgcagcggc ataccaactt tacccatacc cacttccagc
    18841 tcatccgccg tcgcctgaat agcgtgatga acgttttcag cggtccagtc agtaatcgcg
    18901 gccagtttgt cacgaaccac ttccagcggc tgacgcgcta ccggacgcag atgttttttc
    18961 gccgcatcgg catcgaactc agcaaaatct tcgtagaaat aacggcagct ctgtgccatc
    19021 tctttcagcg tcttgcagcg ttcgcccagc agtttcacca gatcagccag ctgcgggccg
    19081 ttacgggtat cgatattttc ctgctcaatg tgccactgta agtgagtagc aacatactcc
    19141 ggcggcagcg cgttaatgta gtgatggttc agccacagca gcttgtcggt gttgaacgca
    19201 ctggcagatt tgctgacggc attcaaagtg aagtatttga tcatctcttc acgagtgaag
    19261 atttcctgat cgccgtggga ccagcccaga cgcaccagat agttcagcag tgcttctggc
    19321 aaataaccgt catcacgata ctgcattacg ctgactgccc cgtgacgttt ggacagtttt
    19381 ttaccgtcat cgccattgat catagaaacg tgcgcgtaaa ccggcaccgg cgctttcagg
    19441 gccttaagaa tgttgatctg gcgcggcgtg ttgttgatat ggtcttcgcc acggataacg
    19501 tgggtgattt ccatatccca gtcatcgaca accacacaga agttataggt tggggaacca
    19561 tcggtacggc ggataataag atcgtccagt tcctggttgc tgaactcgat cggaccacgg
    19621 atctgatcgt caaaaacaac agaaccttcc tgcgggttag caaaacgtac aacacacggt
    19681 tcatcatcag catgatgctc atggctgtgg cggcagcgac cgtcataacg cggcttctca
    19741 cctttcgcca tttgctcttc gcgcagcgct tccaggcgct ctttagagca atagcattta
    19801 taagcagtgc cctcttccag catctgatcg atcaccgcgt tgtagcgatc aaaacgtttg
    19861 gtctggtagt acggaccttc atcccactcc aggctcagcc agttcatgcc atccataatg
    19921 gcttcgatag cttccggcgt ggaacgctca agatcggtgt cttcaatacg cagcacgaac
    19981 tcaccgccgt ggttacgtgc aaaaagccag gagtaaagag cagtacgcgc gccgccaacg
    20041 tgcagatagc ctgttgggct tggcgcgaag cgagttttga ttttcatgaa atggccttac
    20101 gtttagaaag atgccgacaa ccggcaaatc ctggaaaaat tgagtgggcg atattctatc
    20161 actcccgcct gattcctcaa tgtagttcgg gtttctacct tacgcattat tgttttcgtt
    20221 gacaaattgc gcaacacggt ctgttttgcg atcatcttgc ttaaatttac gacgaacgaa
    20281 caatttcttt agaaaatgcg ttgactcatt ttgaactctc cctataatgc ggctccacac
    20341 agcgggggtg attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt
    20401 cgatcccgtc atcacccacc aactacttta tgtagtctcc gccgtgtagc aagaaattga
    20461 gaagtgggtg attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt
    20521 cgatcccgtc atcacccacc aatttctcgc cagctaaatt tcttgtaaaa atgtgaagta
    20581 ccgaagtggg tgattagctc agctgggaga gcacctccct tacaaggagg gggtcggcgg
    20641 ttcgatcccg tcatcaccca ccacttcggg tcgttagctc agttggt
//
LOCUS       DAJVRF010000068        20260 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJVRF010000068 DAJVRF010000000
VERSION     DAJVRF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.068"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(35..139)
                     /locus_tag="OQN13_002267"
     CDS             complement(35..139)
                     /locus_tag="OQN13_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1615611.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            309..755
                     /locus_tag="OQN13_002268"
     CDS             309..755
                     /locus_tag="OQN13_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCS1615612.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(712..1533)
                     /gene="nimR"
                     /locus_tag="OQN13_002269"
     CDS             complement(712..1533)
                     /gene="nimR"
                     /locus_tag="OQN13_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCS1615613.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            1630..2811
                     /gene="nimT"
                     /locus_tag="OQN13_002270"
     CDS             1630..2811
                     /gene="nimT"
                     /locus_tag="OQN13_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCS1615614.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            2866..3213
                     /locus_tag="OQN13_002271"
     CDS             2866..3213
                     /locus_tag="OQN13_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCS1615615.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(3235..3489)
                     /gene="yoaF"
                     /locus_tag="OQN13_002272"
     CDS             complement(3235..3489)
                     /gene="yoaF"
                     /locus_tag="OQN13_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCS1615616.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            3672..4697
                     /gene="dgcP"
                     /locus_tag="OQN13_002273"
     CDS             3672..4697
                     /gene="dgcP"
                     /locus_tag="OQN13_002273"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCS1615617.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            4730..4828
                     /locus_tag="OQN13_002274"
     CDS             4730..4828
                     /locus_tag="OQN13_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCS1615618.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            4831..4905
                     /gene="yoaJ"
                     /locus_tag="OQN13_002275"
     CDS             4831..4905
                     /gene="yoaJ"
                     /locus_tag="OQN13_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCS1615619.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(4964..5212)
                     /locus_tag="OQN13_002276"
     CDS             complement(4964..5212)
                     /locus_tag="OQN13_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCS1615620.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(5360..5542)
                     /locus_tag="OQN13_002277"
     CDS             complement(5360..5542)
                     /locus_tag="OQN13_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HCS1615621.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(5546..5905)
                     /gene="yeaR"
                     /locus_tag="OQN13_002278"
     CDS             complement(5546..5905)
                     /gene="yeaR"
                     /locus_tag="OQN13_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HCS1615622.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(6078..6716)
                     /gene="leuE"
                     /locus_tag="OQN13_002279"
     CDS             complement(6078..6716)
                     /gene="leuE"
                     /locus_tag="OQN13_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HCS1615623.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(6843..7766)
                     /gene="dmlR"
                     /locus_tag="OQN13_002280"
     CDS             complement(6843..7766)
                     /gene="dmlR"
                     /locus_tag="OQN13_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HCS1615624.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            7869..8954
                     /gene="dmlA"
                     /locus_tag="OQN13_002281"
     CDS             7869..8954
                     /gene="dmlA"
                     /locus_tag="OQN13_002281"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HCS1615625.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVAIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            9205..10815
                     /gene="yeaV"
                     /locus_tag="OQN13_002282"
     CDS             9205..10815
                     /gene="yeaV"
                     /locus_tag="OQN13_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HCS1615626.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGGAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYPPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            10847..11971
                     /gene="yeaW"
                     /locus_tag="OQN13_002283"
     CDS             10847..11971
                     /gene="yeaW"
                     /locus_tag="OQN13_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HCS1615627.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            12027..12992
                     /gene="yeaX"
                     /locus_tag="OQN13_002284"
     CDS             12027..12992
                     /gene="yeaX"
                     /locus_tag="OQN13_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCS1615628.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(13046..14161)
                     /gene="rnd"
                     /locus_tag="OQN13_002285"
     CDS             complement(13046..14161)
                     /gene="rnd"
                     /locus_tag="OQN13_002285"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCS1615629.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(14243..15928)
                     /gene="fadD"
                     /locus_tag="OQN13_002286"
     CDS             complement(14243..15928)
                     /gene="fadD"
                     /locus_tag="OQN13_002286"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCS1615630.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(16133..16714)
                     /gene="yeaY"
                     /locus_tag="OQN13_002287"
     CDS             complement(16133..16714)
                     /gene="yeaY"
                     /locus_tag="OQN13_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCS1615631.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(16754..17449)
                     /gene="tsaB"
                     /locus_tag="OQN13_002288"
     CDS             complement(16754..17449)
                     /gene="tsaB"
                     /locus_tag="OQN13_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCS1615632.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(17507..19417)
                     /locus_tag="OQN13_002289"
     CDS             complement(17507..19417)
                     /locus_tag="OQN13_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCS1615633.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            19549..19893
                     /locus_tag="OQN13_002290"
     CDS             19549..19893
                     /locus_tag="OQN13_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS1615634.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         4923 a         5241 c         4942 g         5154 t
ORIGIN      
        1 accagaactg ctcaatttcc gccgcactgc gtaactagcc tgtccgttca ataagcagaa
       61 cggacaaaac cagcacaaca gcaatggcaa aaaacgatga cgtgataatc agtgtctcga
      121 cgaacatttg atcgttcata gcatgccccc gggagatagc tgttttcacg ttattgttag
      181 cgtgcacaaa tggcagtttg atgacaattc gcctgatttt ttatgcaaaa aacgtaaaga
      241 ttttctactt ccttcctgca gcaaacgtaa agtaagcagg cttattattt tttggcaagg
      301 aaaccacgat gtttgatgtc actttgctga tcctgctcgg gttagctgcg ctgggcttta
      361 tcagtcataa caccactgtc gccgtttcaa ttctggtgtt gatcattgtc cgcgtcacac
      421 cgttaagcac ctttttcccg tggattgaaa aacaggggct aagtatcggg ataatcattc
      481 tgacgattgg cgttatggcc cccatcgcca gcgggacgct accgccctca accttgattc
      541 actcgtttct gaactggaaa tcgctggtgg caattgcagt aggcgtgatt gtctcctggc
      601 tgggtgggcg cggcgtgacc ttaatgggca gccagccgca actggtcgcc gggctgctgg
      661 taggcactgt tttaggcgtg gcgctgtttc gcggcgtacc ggtcggcccg cttattgccg
      721 cgggtctggt ttcgctgatt gtggggaaac agtagttaat cccgcaatat atcggcctgg
      781 cgtttgcccc aggcctttct taaacatagt aataaaggcc gttgttgaat cataccccag
      841 cgtatgagca actttctgta cagtatcgcc tttcactaat ccctgaagtg ccataatcag
      901 ctgtaactgc tgacgccact ggcgaaaact caaccccgtc tcttttacta tcagtcgcgc
      961 aagattgcgt tcactcatcg caaaaaagcc agcccattgc cctaatgacc cccactcgac
     1021 gggccctttc gccatcatct ccaccatcgt gcggatttta ggatgagaag aaacgggcag
     1081 gtgcagctgt tgttgtggct gctgagggag ttcatcaaac aggacctgaa ttaatctgcg
     1141 ggtcatcggc tcagcacgct gagtcgtcgt cctgtttgcc agggttaaaa tcaattcccg
     1201 gcataagggc gatattttca atgtgcaaca tgttgtcggc attgtcactg cgctgggttc
     1261 gataaacaga aaacagagtt cagcgttagc tgtcacttga ttgctatgtt ccaccccgcc
     1321 cggtatccaa accgcatatt gcggcggcac catccataaa gcattttcca ccgtacaggt
     1381 aattgcacca tgtaatgcta gtatcagctg tcctttgcgg tgctgatgta caggactggt
     1441 taactcatcc gttccggcgt gaatacaaaa tgcgaccgcg gcttcatggt gtcggtcggg
     1501 ttcatagcca ttgagattca acctgtgcat cattttgtcc gaatttagcg ataatttgtc
     1561 attttagctt gattcaacat aacaataaaa acggtaaggt acagcctcgt ttgtaacaat
     1621 gagaagcata tgacctgttc aacttcatta agcggcaaaa acaggattgt ccttatcgct
     1681 ggcattctga tgattgccac aacattacgc gtcaccttta tcggcgcagc accgttactg
     1741 gatacgattc gttccgctta ctcgctgacg acagcgcaaa ccggcttatt gaccaccctg
     1801 ccattattgg cctttgcgct aatctcacct ttggccaccc cggtagcgcg acgttttggt
     1861 atggaacgta gcctgtttgc cgcgttactt ttgatctgtg ctggtatcgc aattcgctct
     1921 ctcccttcgc cttacttatt atttggcggt acagcggtca ttggcggtgg gattgcatta
     1981 ggcaatgtct tactgccagg attaattaaa cgcgatttcc ctcattccgt cgccagactt
     2041 accggcgcat attccctgac aatgggagct gcagcggcac tgggatcggc tatgatagtg
     2101 ccgctggctt tgaacggttt tggctggcaa ggcgcgttgc tcatgctgat gtgttttcct
     2161 ctgctggctc tttttttatg gctgccacag tggcgaagcc aacaacatgc aaatttgagt
     2221 acctcgcgcg ccttacatac tcggggtatc tggcgttcgc cgcttgcctg gcaggtaaca
     2281 ttgtttcttg ggatcaactc actgatctat tacgtgatta ttggctggct tccggcgatc
     2341 ctcatcagtc acggctatag cgaagcacag gcgggttcac tgcatggttt gctgcaacta
     2401 gccacagcag ctcccggttt gctgatccct cttttcttac atcatgtgaa agatcagcgt
     2461 ggtattgcag cgttcgttgc cttgatgtgc gcagtgggcg ctgttgggct ctgctttatg
     2521 ccagcgcacg cgatcacctg gactctgctt ttcggttttg gttccggcgc aacaatgata
     2581 ctggggttga cgttcattgg tcttcgggca agttctgcgc atcaggcggc ggcactctcg
     2641 gggatggcac aatccgtcgg gtatttgctg gcagcctgtg ggccgccgct aatgggtaaa
     2701 atacacgatg ctaacggtga ctggtccgta ccactgatgg gtgttgcaat actttcacta
     2761 ctgatggcga ttttcggact ttgcgccggg agagacaaag aaattcgcta atatccggtg
     2821 ctatagtgac gtgacaaatc atgcgtgaaa gggagaacaa acacgatgaa tattcagtgc
     2881 aaacgcgttt atgatccggc tgaacagagc gatggttatc gcgtactggt cgaccgcctc
     2941 tggccgcgcg gtatcaaaaa aaccgattta gcccttgatg agtgggataa agaaatcacg
     3001 ccgtcaacgg aactgcgcaa agcctttcac ggcgaagtcg tcgattttgc aacctttcgc
     3061 gagcaatatc ttgcagaact ggcgcaacac gagcaagaag gaaagcggct ggcggacatc
     3121 gccaaaaaac agccgctgac cctgctctac gcagcaaaaa acaccacgca gaaccatgcg
     3181 ctggtgctgg ccgactggct acgtagcttg tgattttagt acagcatccg gcggttattt
     3241 ttcaccagcc ggatggtccc gtcgccacaa tgcccattca tcaatcgttt caccgcccgg
     3301 taatttgcaa ttgttgctga ccccttgcgc tgtcgacact ggaatgagcg tcccgccctt
     3361 ctgctggcaa tagaccgacg ccggatttgc cataccaatc tgcggcggtt taggtacttc
     3421 tggctgagaa gaggttgaac aaccagccag gaccagcaag caaggcaaag caaaactgat
     3481 aattttcatt tattgatctc actcatttat ctacgcttag agtatcgcgg tatcgttttg
     3541 ttttgcagca ctatttttat tacattcact caaaacatat tacgtcttgt ttcatctttg
     3601 ttggtgatgt tttatcatgc ctgcaaagat taaataatca gcatttaccc gccgtatcct
     3661 ggagttgttt cgtgtcagat cagattatcg cccgcgtctc gcaatccctt gccaaagaac
     3721 agtcactgga aagtctggtc cgacagcttc tggagatgct ggaaatggtc actgatatgg
     3781 aatcaaccta cctgaccaaa gtggatgtcg aagcgcgcct gcagcatatt atgtttgccc
     3841 gtaacagcca gaaaatgcac atcccggaga attttaccgt ctcgtgggat tactcgttat
     3901 gcaaacgcgc cattgatgaa aactgctttt tcagcgatga agtccctgac cgttggggcg
     3961 actgtattgc ggcacgcaat cttggcatct ccacatttct gagcacacca attcacttac
     4021 cggatggatc attctatggc acgctttgcg ccgccagcag tgagaagcgc cagtggagtg
     4081 aacgcgcgga acaggtttta cagttattcg ccggactgat tgcacaatat attcaaaaag
     4141 aggcactggt tgaacagttg cgcgaagcca atgctgcgct gattgcgcaa tcgtataccg
     4201 actcgttaac cgggttgccg aatccgcggg cgatttttga aaatctgacg acgctgtttt
     4261 ctctcgcccg gcatcttaac cataagataa tgatcgcgtt tatcgatctg gataacttca
     4321 aattaatcaa tgatcgtttt ggtcataata gtggcgatct gtttctcatt caggttggcg
     4381 agcgccttaa tacgctccag caaaatggcg aagttattgg tcgtctcggc ggtgatgagt
     4441 ttttggttgt ttcactaaac aacgagaatg cggatatttc gtcgctgcga gaacgcattc
     4501 agcagcaaat acgtggagaa tatcacttag gtgatgttga tttgtattat cccggtgcca
     4561 gtcttggcat agtagaagtc gatcctgaaa caaccgatgc agacagtgcc ctgcatgctg
     4621 ccgatattgc gatgtatcag gagaaaaaac acaaacagaa aacacctttt gtcgcgcatt
     4681 cagcgccaca ttgctgaggc gtattcacat ccttttgatt ggtgataaca tgcgaatcgg
     4741 tattattttt ccggttgtaa tctttattac agcggtcgta tttttagcat ggttttttat
     4801 tggcggctat gctgccccgg gggcataaag atgaaaaaaa caacgattat tatgatgggt
     4861 gtggcgatta ttgtcgtact cggcactgag ctgggatggt ggtaacgtca cctctaaaaa
     4921 atagcaaagg ctgcctgtgt gcagcctttg tgcaatttaa gcgttaactt ttaatcttcc
     4981 tgtagataaa tagcacgaca atcgcaccaa taacggcaac cacgaagctg ccaaaattga
     5041 agccatcgac tttaccaaag ccaaacagcg tgctgatcca tccgccgact acggcaccga
     5101 ctatccccag caggatagtc ataaagaatc caccaccatc tttacctggc atgatccact
     5161 tcgccagaat accggcaata agcccaaaaa taatccatga cagaatgccc attgtttcct
     5221 cacttatctg ttttgcatta gcgggttagt cgctgataaa aagcatagca caacatcggg
     5281 agggcaagat ttgtgacgag catcacggag gatttttttg cgatggcgca gaaattgcgc
     5341 catcaacgat cagtgataat taccaaccac aaacatcatg ttcgttttcc gtgtcataag
     5401 aacgcacggt attcaccaga tcttttatca cttcagccgc cacttctggc accagcaaag
     5461 tcatcggcgt ctctgtttca taatcgacag aaacgccatt actgttattg gtgacggtca
     5521 cggtatacgt tgctttgccc atgattcatt tcccgttatg aatgactttc cgttgttgcg
     5581 caccttccat caggacttca ggagccacga agaagtcaat gttgaaataa gtatcgtcag
     5641 tcatggcttc aatgttgtgc cacttttctg gagggaacac ctcaaactgc cccgcttcga
     5701 taaggatcac ctgatcaggc tctgcactgt gttcatcagc gtagccgaga tatttgaccg
     5761 ccccatgcat aacggaaagg cgtgggtaaa cccccgggcg cgttccttta tcaagatgac
     5821 gttcgaatat tccggcaggc gcagtttgtt tattccagaa aggcgttgag cgcgtatgaa
     5881 tataattctg tgggatttgc agcatccttt tccctccttc ggtgaatgcg ctgaaaacgg
     5941 tttattccag ccgtttcagg gtacgcctga taatttgcat tttaaatacc atttattggt
     6001 tactttttag caccatatca gagaaaaatc agggaggatt acaagtggga agcccgcgac
     6061 aacgcgggct gaatgtatca ggattgcagc gtcgccagtc gggcggcgaa acccacgaac
     6121 atcaaaccaa tcagtgagtt gccaacttta gccagtttct ttttggtacg tatgtactgc
     6181 gtgacaaaag caccagaaat aatcaggaag ctcaaatagc agaaactcac cagttccagc
     6241 gtcgtcgcca gaataaagaa tgaaattccc gtatgtgggg cattaacatc gataaactgt
     6301 acgaaaaacg acacatagaa caaaatggct ttcggattag tcaggctcaa aattaacgcg
     6361 cgtttaaaaa tggccccgta ttggggctca tcggatttgg cctcgttatt tttacccttc
     6421 agggtcgcgt agagaatttt actccccaga tagagcaaat aaaacgcacc aagataacgt
     6481 acaatgttga ataatatcgg ggtggtctta attaatgtcg ccactccagc ccatgccaga
     6541 aacatcaata ctgcatcgcc aataaataca ccgcaggccg caagataacc gcctttcata
     6601 ccgctactga cgctattttt gagtacaaac agggtatttg gccctggcac caacacaata
     6661 aaaatggccc caaccagata ggtccagtaa ttcagaaccc cgtattcagc gaacacatta
     6721 acctctttaa ttatctttcg atcatgcgcg attaaaggtg aatatgctaa ccaatctgta
     6781 gcggcttaga aaggagaaaa tcaggtttta acccgatatc aacccgataa ttgaataatt
     6841 aatcaggcat gctccagtga aaaattcggg tagtgctcgg caaaatactg gcgtaaaaat
     6901 tctatcgtta tccgcacttt cgctgacgtc gccagccttg aaacataaac ggcccagacg
     6961 ttcgctggct ggtaatattc cggtagcact tgcactaaat gaccactggc aatgttttcg
     7021 ctaacatccc accaggagcg cagagcaatc ccctgcccat cgagacacca ctgatggaca
     7081 atttctccat gattagacga cagtgggccg gtgactttaa tcgcatggtg gccttcttta
     7141 ttgcgtaatt gccagacacc aaaaggatga tcgcgctctt taatgaccag gcacggcaat
     7201 gcggaaagat cggttaaatg tttcggcgcg ccatgttgcg caataaattc cggcgacgcg
     7261 cagagaatgc ggtaattagt cgccagtttg cgggcgatta aattaggggc gatgtcatcg
     7321 ccaatgcgaa tatcgagatc aacaccttca ttgaccaaat cgaccaaccg gtcttccaca
     7381 tcaaaacgta attccagttg cggatacgcc ttagccagcg ctgataacgc cggagccacc
     7441 acctgtcgtc caaaaccaaa gctgctgata atacgcagca tcccctgcgg cacctgacgc
     7501 acgtcagaaa gttcgtccat catctgaccg acatcctgca aaatccgctg cgcccattca
     7561 taaatccgct ctccttcttc ggtaatggtg acgcggcggg tggtgcggtg taacaacacc
     7621 acgtttagcg tttgctccag caaggcgatg cgcttgctga cgaacgccgg tgaaacgccc
     7681 agttcttccg ccacggcggc aaaaccagcc cggcgagcca ccagcataaa gacgcgcaaa
     7741 tcgttcagca gcggtaaatt attcatgatt cgtgttttat gtttcaccag ttacggggat
     7801 taattccttt tcagtcaatt ataggatggt gatgttgtca attttgatgg tcaggaagtg
     7861 agaacccaat gatgaaaacg atgcgtattg ctgcgatccc gggagatggg attggcaaag
     7921 aagtccttcc tgaagggatt cgcgtattac aggctgccgc tgagcgctgg ggcttcgact
     7981 tgagttttga gcaaatggag tgggcgagct gcgagtatta cagccatcac ggtaaaatga
     8041 tgccggacga ctggcatgag caacttagcc gtttcgacgc catctatttt ggtgccgtcg
     8101 gctggccgga taccgttccg gaccatattt cgttgtgggg ttcgctgctg aaatttcgtc
     8161 gtgaattcga ccagtacgtc aacctgcgcc cggttcgtct ctttcctggc gttccctgcc
     8221 cgctggcggg aaaacagcct ggcgacatcg atttttacgt ggtcagggaa aacaccgaag
     8281 gcgaatattc ctcgctcggc ggtagagtga atgaaggtac agagcatgaa gtcgccattc
     8341 aggaatcggt atttacgcgt cgtggtgtcg atcgcatttt gcgttatgcc ttcgaacttg
     8401 cgcaaagccg cccacgtaag acgctaactt ctgccactaa atcaaacggt ttagccatca
     8461 gcatgccgta ctgggatgag cgagtggaag caatggccga gaattacccg gagatccgct
     8521 gggacaagca gcatattgat attctctgcg cgcgttttgt gatgcagccg gaacgcttcg
     8581 atgtggtggt ggcgtccaat ttgtttggcg atatcctttc cgatcttggc ccggcctgca
     8641 ccggcaccat tggcattgcc ccatccgcca acctgaatcc ggaacgcact ttcccgtcgc
     8701 tcttcgagcc tgtccacggt tccgcgccgg atatctacgg gaaaaatatt gctaacccta
     8761 tcgccacaat ttgggccggt gcgatgatgc tcgattttct cggcaatggc gatgagcgtt
     8821 tccagcaagc gcataacggt attctggcag cgattgaaga agtgattgct cacgggccga
     8881 aaacaccgga tatgaaaggc agtgccacca cgccacaggt tgccgacgcg atttgcaaaa
     8941 ttattttgcg ttaaggtcaa accagtttat ttgaaccgcg tcactgacgc ggttttttta
     9001 ttcgttcttt gcagttaata acctgcgtca tttcaccttt tattgtttcc gtttcgtgtt
     9061 ttatggcttt ccgtattctt aattgtttaa tttatgtaac atgcaaataa tcaatttgtt
     9121 aaacacaaca tacaactcat taccctgcaa ataattacaa ctggcaaaag ccagagacgg
     9181 gcttttatgc ccaggagatg actcatgatg agcaatgtta agaaaaaaga tgtgccgctg
     9241 ataagcatca gcctggtggc cattcttttc atcgcagctg cattaagcct tttcccacaa
     9301 caatcggccg acgcggccaa cacaatatac acttttgtta cgcgtacgtt aggttccgcc
     9361 gtacaggtat tggttttgct ggcaatggga ctggtgattt atttagccac cagtaaatac
     9421 ggcaatattc gtcttggcga aggaaaaccg gaatacagca cgctctcctg gctgtttatg
     9481 tttatttgtg ccggtttagg ttcttctacg ctttattggg ggggtgctga atgggcctat
     9541 tattatcaaa cgcctggatt aaatatcgca ccacgttcac aacaggcact cgaatttagc
     9601 gtcccttact ccttcttcca ctggggcatc agcacctggg caacttatac gctggcctca
     9661 ttaatcatgg cttatcactt tcatgtgcgt aaaaacaaag gtctgagtct ttccggcatt
     9721 attgccgcga ttaccggcgt tcgcccgcaa ggcccatggg gaaaactggt tgatttgatg
     9781 tttctgatcg ccactgtcgg cgcactgact atttcccttg ttgtcaccgc agcaaccttt
     9841 actcgtggac tttccgcgct gaccggttta cccgataact tcaccgtgca ggcatttgtg
     9901 atcctgcttt ccggcggcat tttttgccta agctcgtgga ttggtatcaa caacggtttg
     9961 caacgtctga gcaaaatggt tggctggggc gcgttcctgc tgccattact ggtgctgatt
    10021 gtcggtccaa ccgaatttat taccaacagc atcatcaatg ccatcggcct gaccacgcaa
    10081 aacttcctgc aaatgagctt attcaccgat ccgcttggcg atggttcatt tacccgcaac
    10141 tggaccgttt tctactggct gtggtggatc tcataccccc ctggcgtagc aatgtttgtc
    10201 acccgcgttt cccgcggtcg taagattaaa gaagttatct ggggactgat cctcggcagc
    10261 accgtcggtt gctggttctt cttcggcgta atggaaagct atgccattca tcagtttatc
    10321 aatggcgtaa tcaacgtccc acaggtgctg gaaacactgg gcggcgaaac agctgtacag
    10381 caagttctga tgtcgttgcc agccggtaaa ttgttcctcg ccgcatacct gggcgtgatg
    10441 attattttcc ttgcctcgca tatggatgca gtggcctaca ccatggcggc gaccagtacg
    10501 cgtaatctcc aggaaggtga cgatcctgac cgtgggctgc gtcttttctg gtgcgtggtg
    10561 atcactctga tcccgctttc catcttgttt accggtgctt cgctggaaac gatgaaaacc
    10621 accgtcgtgc tcacagccct tcccttcctc gtcattttac tggtgaaagt cggcggattt
    10681 attcgctggc tgaaacagga ttatgccgac attccggctc atcaagttga acattatctc
    10741 ccgcagacac cggttgaagc cctggaaaaa acaccagtgc tccctgcggg aaccgtattc
    10801 aaaggcgaca actgagcgcc atcatcctaa cgataaaggt atccctatga gcaatctgag
    10861 ccctgacttt gtactacccg aaaatttttg cgctaacccg caagaggcgt ggaccattcc
    10921 tgcccgtttt tataccgatc agaacgcgtt tgaacacgaa aaagagaacg tcttcgccaa
    10981 aagctggatt tgcgtcgctc acagcagcga actggcgaat gccaatgatt atgtgacgcg
    11041 tgagatcatt ggcgaaagca tcgtgctggt acgcggtcgt gataaggttt tgcgcgcgtt
    11101 ctataacgtg tgtccgcacc gtggtcatca gttgttgagc ggtgaaggaa aagcaaaaaa
    11161 tgtgattacc tgcccatatc acgcatgggc attcaaactc gatggcaatc tggcccatgc
    11221 acgtaactgc gaaaacgtcg ccaatttcga tagcgacaaa gcgcaactgg ttccggtgcg
    11281 tctggaggaa tatgccggat tcgtcttcat caacatggac cccaacgcca ccagcgtaga
    11341 agatcagtta cccggtctgg gcgcgaaagt gctggaagcc tgcccggaag tccacgatct
    11401 gaaactggcg gcccgcttta ccacccgcac gcctgccaac tggaagaaca ttgtcgataa
    11461 ctatctcgag tgctatcact gtggtccggc gcatccaggt ttctccgact ccgtacaggt
    11521 tgatcgctac tggcacacca tgcacggtaa ctggacgctg caatacggtt tcgccaaacc
    11581 gtccgaacag tcgtttaaat ttgaagaggg tacggatgcg gcattccacg gtttctggct
    11641 gtggccgtgc acgatgctga acgtcacccc gatcaaaggg atgatgacgg tcatttatga
    11701 attcccggtg gattctgaaa ctaccctgca aaactacgat atttacttca ccaatgaaga
    11761 gttaaccgac gagcaaaaat cgctgattga gtggtatcgc gatgtgttcc gtccggaaga
    11821 tttacgtctg gttgaaagcg tacagaaagg gctgaaatcg cgtggctatc gtggtcaggg
    11881 gcgcatcatg gccgacagta gcggtagtgg catttccgaa catggtatcg cccatttcca
    11941 taatctgctg gcgcaggtgt ttaaggacta atgacatcgg cggcggtatt ttccgccgct
    12001 gggctgattt ttgatggagt acagcaatgt cagactatca aatgtttgaa gtacaggtga
    12061 gtcaggtgga accgcttacc gaacaggtga aacgcttcac gctggtggca accgatggca
    12121 aaccgctgcc tgcgttcacc ggaggaagtc acatcattgt gcagatgagc gatggtgata
    12181 accagtacag caatgcgtat tcactactga gttcgccgca tgacacctct tgttatcaga
    12241 ttgccgttcg gctggaggaa aactcgcgcg gcggttcccg ctttttgcat cagcaggtaa
    12301 aagtgggcga tcggttaacg atttcaacgc ctaataacct gtttgcgcta attccctcag
    12361 ccagaaagca tctgtttatc gcgggcggta ttggtatcac ccctttcctg tcgcacatgg
    12421 cagagttgca acacagcgac gtcgactggc agctacatta ctgctcgcga aatccagaaa
    12481 gttgcgcatt tcgtgatgag ctagtccagc atccgcaggc tgagaaagtc catttgcatc
    12541 attcgtcaac cggaacacga ctggaattag cgcgattatt ggcggatatc gaacctggca
    12601 cacacgttta tacctgtggc cccgaggcgc taaatgaagc ggtaagaagt gaagctgcgc
    12661 gtctggacat cgccgccgat acgctgcact ttgagcaatt tgctatcgaa gacaaaaccg
    12721 gcgatgcctt taccctggtg cttgcccgtt ccggaaaaga gtttgtggtg ccggaagaga
    12781 tgactatttt gcaggttatt gaaaataata aagccgcgaa agtggaatgt ttatgtcgtg
    12841 aaggggtatg cggaacctgc gaaacagcaa tactggaagg tgaagctgac catcgggatc
    12901 agtattttag cgatgaagag cgtgccagcc agcaaagtat gttgatctgt tgttcgcgtg
    12961 cgaagggtaa acgcctggtg ttggatttgt agtttgccaa agccggatgt ggcgctgagc
    13021 gcgcccagtc cggcttcgga agattttact gcggatattc ctgcaataaa ttgtgtaatg
    13081 cttccgccat cagctcacca cgccagccgg aaatcagctc cggcaaattg ttctgcggtt
    13141 tcagtttcca gtgccagttc agcagttggt tgatttgccg acgcgatgcc agcaattcgg
    13201 cgctgatctt atgcgtttcg ctcacgtcag taatcagcga cttaatcgct ttaaacgctt
    13261 tacgataacc cggcatgtcc atcaggttaa gcatcggctg cggtaaggcc tcttccggca
    13321 atgtctgcgc tttttccacc agcgccagca gcgttttacc gtgaaagcgg atttcgctac
    13381 cggataaacc caggctgtcc agttcgccta aacttcccgg catataacgc gcgaccgacc
    13441 acaaatgctc ttcacgcacg acaaagttca ccgccagatc acgctcacga gccttgcgca
    13501 gtcgccagtc ggctaacagt tgcagacagg ccagttggcg tgtgcgtaat tgccaggcat
    13561 tggtgatatc acgccaggca tcttccggcg caacgacttc ctgacgacgc atttgcatca
    13621 ggcggcattc atccagcgcc gcaggtagcc agccggaggc ctccgtttct accataagct
    13681 tggcggtgat cggtaacaga taccagacat ccgccgctgc gtattcacac tgacgttcgg
    13741 tcagcggtct ggccagccag tcggtgcgcg attcactctt gtccagcgta acgccggaat
    13801 actcttccac catggaagcg aaaccccatg acatcgggcg tccgcagaag gcagcaagga
    13861 tttgcgtgtc aatcaggggt tgtggtaatt cgccaaagac attgaggaac acttccagat
    13921 cttcactgcc tgcatggaga aattttgtga tggacggatc gcgcaggatc gctttcagcg
    13981 gtgaccagtc ggtgatcccg agaggatcaa ttagcgccag atgctcacca tcgaaaagtt
    14041 gaatcaaccc cagctgcggg taataagtgc gcgtacgaac aaattcagta tcctgggcta
    14101 tcgccggaaa gacacggacg gcttcacaca aagaagccag cgcatcgtcc gtggtaatca
    14161 tttggtaatt caaattgtat tctctttagt gggcgtcaaa aaaaacgccg gattaaccgg
    14221 cgtctgacga ctgacttaac gctcaggctt tattgtccac tttgccgcgc gcttcgtcac
    14281 gtaattctcg tcgcaaaatt tttccgacgt tagatttcgg taactcatca cgaaactcca
    14341 ccagcttcgg tactttgtag cccgtgagct gacggcagca aaaggtcacc agtgactctt
    14401 cggtaagcga tggatctttt ttcactacga agattttcac cgcttcacca ctggagccgg
    14461 aaggtacgcc aacagccgcg acttcctgta cgccaggatg ctgcatgacg acatcttcaa
    14521 tctcgttggg atagacgtta aaaccggaaa ccagaatcat gtctttttta cgatcgacaa
    14581 tgcgcaggaa cccttcttca tccatcaccg cgatgtcgcc ggtgtgtaac cagccatttt
    14641 tgatgatctc atcggtagca tcgggacgct gccagtaacc cagcatcacc tgcggtcctt
    14701 tgacacaaag ctcacccggt tgacccggtg gtacttcatt atcgtcgtca tccaccagtt
    14761 tggcttccgt cgacggcacc ggcaaaccga tgctaccact atgataatca atatcatatg
    14821 ggttaacgct gaccagcggc gcacactcgg taagaccata gccttccagc agatactgtc
    14881 ctgtcagttt cacccaacgc tctgccacca cttgctgcac tggcatccca ccgcctgcgg
    14941 aaagatgcag actggagaaa tccagctgct ggaactcttt attgttcagc aacgcattga
    15001 acaaggtgtt aacgccagtg atagcggtaa acggatattt cgctaactct tttaccaacc
    15061 ctggaatatc gcgcgggtta gtgataagca ggttctgccc acccagttcg ataaacagca
    15121 ggcagttaat ggtcagggca aaaatgtgat acagcggcag tgccgttact accagttctt
    15181 tacctggatg cagcaacggc ccataggtgg cgttgacctg ttccaggttc gccagcatat
    15241 tgcggtgagt cagcatcgcg cctttcgcca cacctgtggt gccgccggtg tattgcagga
    15301 aggctaaatc ttccggcacc agttcgggtt tgacgtactg catccggtag ccgttatgca
    15361 gtgcgctacg aaatgaaatg gcatctggca gatggtattt cggcaccaaa cgcttgatgt
    15421 atttaacaac gaaattgact accgtgcctt ttgccgtaga tagctgatcg cccatacggg
    15481 tcagaattac gtgctgaacg gcggttttat caaccacttt ttccagtgtg tgagcaaagt
    15541 tagacacgat aacaatcgcc gatgcgccgc tatcgttaag ctgatgctca agctcacgcg
    15601 gggtatacaa cgggttaacg tttacgacga tcatcccggc acgcaaaatg ccaaacagcg
    15661 ccaccggata ttgcaataaa ttaggcatca tcaacgcaac gcgatcgcct ttcttcagcc
    15721 ccaacccttg ttgcaaataa gcggcaaacg cgcgactgcg ttcttccagc ttgcggaagg
    15781 tcattacctc ccccatattc acaaacgcag gctgatcggc gtagcggacg accgactgct
    15841 caaacatatc tatcagagat tgataacggt cagggttgat ctccgtcgga acatccgcgg
    15901 gataacggtt aagccaaacc ttcttcaatt cttcacctct aaaatgcgtg ttcgtcgtca
    15961 tcgcaacccc aagtgataaa catgttgtta acataacatt aactcatctt accagcttga
    16021 taattagaca acgaaaaggt tgcgaagcgc gtcactattt atttttatag ttgccgtatg
    16081 aacgcagaaa cagcggacca gccgctgttt ctttttcttt aaaaacaact tgttactcgg
    16141 ttacaattgt ttcaactctt gcagggccag gattatacca accccatccg ccgtaaccat
    16201 agggccagcc gcgtccacca taaaaccatg gatcaattgg ctgcggcggc ataatcacct
    16261 gttgagtgat atgccagcgt ttgtaacctg ttacctgcat aaccataaat ttatatggtg
    16321 cattatttac tttgccatcc actgtgccag tgattggccc gacaaccgtc accagttgtc
    16381 cacgaaaatc gaccggatca agaaaaccgt tcacatcggc ataaatgcgc ccgcgtgaag
    16441 gttctcccaa ggttggtctt gctccgctgt ccagcggtac ggtagcaatt tccaggcggg
    16501 ttttcccttg ctggttttgt accacaacca ctttgccgcc aaagcgcgcc tcctgaccaa
    16561 catataaatc tggcgcattc atcacgcgta ccagatcttg ctgtggtgtt ggactgctgc
    16621 ctttaatggc gtccggcaca gtgacacaac cgctcagcat tagcgcaaac gtacctgcca
    16681 gtatgccttt gataacattc ttttgaaccg ccatgatgcg actccttttt ttctcagggc
    16741 atactcttaa gattcattct ttgcccggaa gtttcttcca tgcgacgttg ttacgtaaat
    16801 aaaccggttc ggcatgttcc accgccaccg ttttaccctc ggcaaacatc tgacacgcaa
    16861 tcggcagcat atcttccgcc gctggcagta aaacttctcc gtcgcgcaaa accagcccgc
    16921 tctctttacg gagatccggc caggcttgcc agcccgtccc taccgttacc cattcgccgg
    16981 aaagctgttg cattcgttca tgaacgagtt cgggtttgag tacggcttcg gtttcttcac
    17041 cgcgccagat accgttttca tcacgctgat attcggccca gtaaacttcg cccattcgcg
    17101 cgtcaatggc tgccagcacg cgggttgcgc cgtttttgcg ccacgccccc tgcgccatcg
    17161 tcattagcgt agagacacca atcatcggta actccgcgcc aagcgccagc ccttgcgcaa
    17221 taccaatacc aatgcgcacg ccagtaaagc taccggggcc gcgcccgtaa gccagagcgt
    17281 ttatgtcagt cagggaagtt ccgctggtgg tcaggatatc ctgcaccatc ggtaagattc
    17341 gttgagtatg ttcacgaggg caaagctcaa aatgagcgtt gacattaccg tcgttccaca
    17401 gagcgacaga gcagacctct gtcgcggtat cgatagccag aattcgcatg ggtcttcgtg
    17461 cttagatcaa taaaaaggcg cgcatcatac catactccgt aacaaattac ctggaggatg
    17521 gtatcgcaag gaaacgaacc gcacgggcaa tgtcacgggt gcgtggcgcg ggcggcagac
    17581 tggcgagaaa cgtcgcgccg taaggacgca tcaccagccg attgtcacaa attaccagca
    17641 cgccgcgatc gtcggcgtcg cgaatcagtc gccctactcc ctgcttgaga gtaatgaccg
    17701 catccggcag ctgcacttca tcgaacgggt cgccaccgcg caaacgacaa tcttccatgc
    17761 gcgcttttaa cagtagatca tccggcgagg taaacggcaa tttgtcgata attaccaatg
    17821 acaatgtatc gccacgcacg tccacccctt cccagaaact actggttgcc acaagaagcg
    17881 cgttaccggc gctgacaaat tgctgcaaca gttgcccttt gctggtttcc ccctgcaaca
    17941 aaacgggaag cgtcatggta gcgcggaact gctcggccag atcgcgcatc atggcatgcg
    18001 aggtacaaag cataaaacaa cgaccgttgt tagcttcgat gatcggtcgc agcattgccg
    18061 ccagttgccg tgcacaaccc ggttgattgg tttgcggcag gttgcgcggc acacagagta
    18121 acgcctggcg gctgtaatca aacgggcttg gcaatagcaa cgactccgcc tgttctatcc
    18181 caagccgcga ggtgaaatga tgcagatcgt cgttcaccga cagcgttgct gaggtgaaga
    18241 tccagctacc gggcttttgc gccattaact ctttgaattt atccgccacg ctgagcggcg
    18301 tgagagccag agtaaaatgg cgcgaagtgc attcgtacca gtagctgtag cccggctgat
    18361 tgatctcttt tagccgcttc agccgtgtgc gatacaacgt ggcgcgctca aatgccgcat
    18421 ccagcaaggc ggaacgcccc agtgacagtt tcgccacgtc ataacaaagt tccagggtgt
    18481 catcgagcag taaaaatgcc cgctgaattt gcggattagc taacagctca cgcaggttac
    18541 cgcgataacc tggctcaccg agttgcagac gaaaatcctg tgcactctgg gcaagacgat
    18601 cagcgcactt ttgtaactgc tgggtgtctt ttaattcggt gcggtaggcg atggtgatgt
    18661 cttttgccag gtcgagcagt tgtcgactgg agagtgactg accaaaatac tggctggcaa
    18721 tgtccggtag ctggtgggct tcgtcgaaga tcatgacgtc cgcttcaggg atcaactcgc
    18781 caaatccact ctctttaacc accatatccg ccagaaagag atgatggttt accaccacca
    18841 catcggcgtc catcgctttt ttacgtgctt tgaccacaaa gcaatcttta tacatcgggc
    18901 agtcgctgcc aagacagttg tcgttggtgc tggtgaccag cggccacgcc tgtgaatctt
    18961 ccgccacgct gacgcaggtg ctgatatcac catcgactgt ttgattagac caggagcgca
    19021 gcaggatcac atcgcttaag atttgtaccg gcagatcgcc ccccgccagc gcctgctgtt
    19081 cgagacgttc gaggcagagg tagtttgagc gccctttcag cagcgccacg ttgcccgtat
    19141 atttcaatgc ctttgagact gttggcaaat cgcggctgta gagctgatcc tgcaacgctt
    19201 ttgagccggt cgagataatg actttctttt tcgcccgcag cgcaggagcc aggtaagcgt
    19261 aggttttgcc cgtaccggtt cctgcttcca ccaccagcgg ctggcctttt tctatcgcct
    19321 gggtgacggc taccgccatc tgtcgctgtg gttctcgcgg cttaaagcct ggtatcgctt
    19381 tcgccagctg accgtctggt gcaaaatcgt ccgtcacact accccctgtt gatttgaaca
    19441 gggattatgt caggatgagg gcgctttcgc cagttgaagt ggtgacggca acctcacatt
    19501 gtggcagtct ttgcagcacg aaaatggaag tataatgagg acaaaatgat gactatcgtt
    19561 cgtatcgatg ctgaagcccg ctggtctgat gtagtaatcc acaacaacac gctctactac
    19621 actggtgtac cggaaaacct cgacgccgat gcctttgagc aaaccgccaa cacgctggca
    19681 cagattgacg ccgtgctgga aaaacagggc agcaataaat cgagcattct ggatgccacc
    19741 attttcctgg ccgataaaaa cgacttcgcg gcgatgaata aagcgtggga tgcctgggtt
    19801 gtcgcgggtc atgcgccagt gcgctgcacg gtacaagcgg gtttgatgaa cccgaagtat
    19861 aaagttgaaa ttaagattgt ggctgcggtg taagctttat cgaagcaaaa taagtcagac
    19921 gataatttat cgataatact ggtcggtttt acataaatcg accagagaat aagatattac
    19981 tgttgatgct tctgctaatt cattatttat atttataatt tcaattttat ctataaaact
    20041 aactatataa ataaacataa ccatgccgtt gtgttcgccc tttgattagc aactctggtt
    20101 ttctttctta gctgaacaat ctggttatag cgtgcggact gaccgttaat tttctccttc
    20161 ttactggata acacaccatg agctgctacc tttaaaacac ctgaatgtca acaggttaac
    20221 tcgtgccata tcgttataat ccctttgata ttagatgcaa
//
LOCUS       DAJVRF010000069        20231 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJVRF010000069 DAJVRF010000000
VERSION     DAJVRF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.069"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            690..1460
                     /locus_tag="OQN13_002291"
     CDS             690..1460
                     /locus_tag="OQN13_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCS1615635.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1451..1924
                     /locus_tag="OQN13_002292"
     CDS             1451..1924
                     /locus_tag="OQN13_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCS1615636.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            2032..2571
                     /gene="dnaT"
                     /locus_tag="OQN13_002293"
     CDS             2032..2571
                     /gene="dnaT"
                     /locus_tag="OQN13_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCS1615637.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2574..3311
                     /gene="dnaC"
                     /locus_tag="OQN13_002294"
     CDS             2574..3311
                     /gene="dnaC"
                     /locus_tag="OQN13_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCS1615638.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3360..3854
                     /locus_tag="OQN13_002295"
     CDS             3360..3854
                     /locus_tag="OQN13_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCS1615639.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            4108..6399
                     /gene="opgB"
                     /locus_tag="OQN13_002296"
     CDS             4108..6399
                     /gene="opgB"
                     /locus_tag="OQN13_002296"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCS1615640.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6538..7560)
                     /gene="lgoD"
                     /locus_tag="OQN13_002297"
     CDS             complement(6538..7560)
                     /gene="lgoD"
                     /locus_tag="OQN13_002297"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCS1615641.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7699..8613
                     /locus_tag="OQN13_002298"
     CDS             7699..8613
                     /locus_tag="OQN13_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1615642.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8828..10179
                     /locus_tag="OQN13_002299"
                     /pseudo
     CDS             8828..10179
                     /locus_tag="OQN13_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10228..11883)
                     /gene="tsr"
                     /locus_tag="OQN13_002300"
                     /pseudo
     CDS             complement(10228..11883)
                     /gene="tsr"
                     /locus_tag="OQN13_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(12002..12448)
                     /gene="hpaR"
                     /locus_tag="OQN13_002301"
     CDS             complement(12002..12448)
                     /gene="hpaR"
                     /locus_tag="OQN13_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCS1615643.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            12720..14009
                     /locus_tag="OQN13_002302"
     CDS             12720..14009
                     /locus_tag="OQN13_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCS1615644.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            14006..15472
                     /gene="hpaE"
                     /locus_tag="OQN13_002303"
     CDS             14006..15472
                     /gene="hpaE"
                     /locus_tag="OQN13_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCS1615645.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15474..16325
                     /gene="hpaD"
                     /locus_tag="OQN13_002304"
     CDS             15474..16325
                     /gene="hpaD"
                     /locus_tag="OQN13_002304"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCS1615646.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16335..16715
                     /locus_tag="OQN13_002305"
     CDS             16335..16715
                     /locus_tag="OQN13_002305"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCS1615647.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            16826..17629
                     /gene="hpaH"
                     /locus_tag="OQN13_002306"
     CDS             16826..17629
                     /gene="hpaH"
                     /locus_tag="OQN13_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCS1615648.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17640..18428
                     /gene="hpaI"
                     /locus_tag="OQN13_002307"
     CDS             17640..18428
                     /gene="hpaI"
                     /locus_tag="OQN13_002307"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCS1615649.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            18603..19979
                     /gene="hpaX"
                     /locus_tag="OQN13_002308"
     CDS             18603..19979
                     /gene="hpaX"
                     /locus_tag="OQN13_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCS1615650.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            19989..>20231
                     /locus_tag="OQN13_002309"
     CDS             19989..>20231
                     /locus_tag="OQN13_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCS1615651.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         4870 a         5429 c         5168 g         4764 t
ORIGIN      
        1 tcttttaacc ctgcttgcat aacaggtatt ttactgataa caattccatt tttgcagcat
       61 cctggcaaca tatttcctcc acatccactg tctaaaaatc atgatggttt attcaggata
      121 ttcattcctt aaatgtttcc agcaaattaa tagattaagc gaataaatta tccttagcat
      181 tcactttcat atcagcatac tcctgctgca caacaactcc actgcatgtg tcagaaggtt
      241 agcatcaaat gataacctgg tgaaatagcc tggagacatt taaaatatct acattaagtt
      301 gttaggaata cttaatgaaa aacagaaatc acattcattt aatttataat taaatgatat
      361 cccttcccgc agaatgaaaa atcacacaac caatatttat tcaatgaaat ctgatggttt
      421 taacttgtta acattaaaaa acgtgcccgg ttatctatta tcctgattcg taaaggccaa
      481 acaataaatt cattatagtg ataatattca tgtgatagtt tgtttgcggc gagagataat
      541 tcgcttttta tcaccgagct tgtcccgata accaggatta atcagcgcaa ttttgatctt
      601 aacctaccgc aaaacaaccg tgcttcatga tacaaatgga ttctcattat ctgaacaata
      661 cgcagcacgt ctatgacaaa gggcgagtta tgcaaactga gcaacagcga gccgtaacac
      721 ggctttgtat ccagtgtgga ttatttcttt tgcaacatgg tgcggaaagc gcgttggttg
      781 atgagctttc ctcacgactg ggtcgggcgt tggggatgga cagcgtcgaa agttctatct
      841 cttcgaacgc catagtgctg acaaccatta aagatgggca atgcctgaca tcgacacgta
      901 aaaatcacga tcgcggcatt aatatgcatg tggtgactga agtccagcac attgtgattc
      961 tggcggagca ccatctgctg gattacaaag gcgtagagaa acgatttagc caaattcagc
     1021 cattacgtta cccaagatgg ctggtagcct taatggttgg cctttcttgc gcctgtttct
     1081 gcaaactcaa taaaggtggc tgggatggtg ccgtcatcac cttctttgcc agtacggccg
     1141 cgatgtatat ccgccagctg ctggcacaac gtcatcttca tccgcagatc aacttttgcc
     1201 tcaccgcttt cgccgccacc accatttccg gattgctttt gcaacttccc actttcagca
     1261 atacacccac tatcgcgatg gccgccagcg ttctgctgct ggtgccggga tttccgttga
     1321 ttaatgccgt cgccgatatg tttaaaggcc acatcaatac cggactggca cgctgggcga
     1381 tcgccagtct gctgacactg gctacctgcg tcggcgtggt gatggcactg acgatttggg
     1441 ggctacgcgg atgggtgtga tcgagtttct gtttgcattg gcgcaggata tgatcctcgc
     1501 cgccattcca gcggtcggct ttgcgatggt gttcaacgtt cccgttcggg cattacgctg
     1561 gtgtgcgctg cttggcgcga ttggtcatgg ttcacgaatg atcttaatga ccagcgggct
     1621 gaatattgag tggtcaacct ttatggcctc catgctggtc ggcaccattg gtattcaatg
     1681 gtcgcgctgg tatctggcgc atccgaaagt gtttaccgtg gcggccgtta tccctatgtt
     1741 cccgggcata tcggcttata ccgcaatgat ttcggcggta aaaatcagcc agttaggtta
     1801 cagcgaaccg ttgatgatta ccctgttaac caactttctt acagcttcat cgattgttgg
     1861 tgcgttatcc atcggtcttt ccattcctgg attatggttg taccgcaagc gccctcgcgt
     1921 ataaaattgc ctcgcttaaa gcacacggat gagagacagc ctcctctcct ccgtgtgtta
     1981 ctataaaagt tatctccctt ctcgttcatc gttccatatt tgagaaacag tatgtcttcc
     2041 agagttttga ccccggacgt cgttggtatt gacgccctgg tacacgatca ccaaaccgtt
     2101 ctggcaaaag ctgaaggcgg tgtggttgcc gtatttgcta acaatgcccc ggcgttttat
     2161 gccgtcacgc ctgcacgcct ggctgaactg ctggcgctgg aagaaaagct ggcgcgtccg
     2221 ggaagcgatg tcgctctgga cgatcaactc tatcaggaac cgcaagccgc tcccgttgct
     2281 gtacccatgg ggaaattcgc catgtatccg gactggcaac ccgatgccga ttttatccgc
     2341 ctggcggcgc tatggggtgt ggcactaaga gagccggtga ccaccgaaga actggcctca
     2401 ttcattgcct actggcaggc ggaaggtaaa gtctttcacc atgtgcagtg gcaacaaaaa
     2461 ctggcgcgca gcctgcaaat cggtcgtgcc agcaacggcg gactgccgaa acgagatgtg
     2521 aatacggtca gcgaacctga cagtcaaatt ccaccaggat tcagagggta acgatgaaaa
     2581 acgttggcga cctgatgcaa cgcctgcaaa aaatgatgcc tgcccatatc aaacctgcat
     2641 tcaaaacggg tgaagaacta ctggcctggc agaaagaaca aggagcgatc cgctccgccg
     2701 ctctcgaacg tgaaaatcgg gcgatgaaaa tgcagcgcac ctttaaccgc tccggtattc
     2761 ggccactgca tcagaactgc tcctttgaga actatcgcgt tgagtgtgaa gggcagatga
     2821 atgcgttaag caaagcgcgc cagtacgtcg aagagtttga cggcaacatc gccagcttca
     2881 tcttttctgg taagccagga accggcaaaa accatctggc ggcggcaatc tgcaacgagc
     2941 tgctgctacg cggtaaatcc gtattgatca tcaccgtggc cgatattatg tcggcgatga
     3001 aagatacctt caggaatagc ggtaccagtg aagaacaact gcttaacgat ctgagcaacg
     3061 ttgatctgct ggtgatcgat gagatcggcg tgcagaccga atcgaaatac gaaaaagtga
     3121 tcatcaacca gatcgtcgat cgccgctctt cttccaaacg cccaaccggg atgctgacca
     3181 acagtaatat ggaagagatg accaagttgc tgggcgaacg cgtgatggac cgtatgcgcc
     3241 tgggtaacag tttgtgggtg atcttcaact gggatagcta ccgtagccgg gtaacaggta
     3301 aagagtatta atccccgttt ctggcggtat actgcgacac aattccacct tgagtaatga
     3361 tgatgaaaac tgttaaacat cttctgtgct gtgccattgc cgccagcgca ttaatttcca
     3421 ccggggtgca tgctgcgtcc tggaaagatg cgctctccag cgccgccagc gaacttggca
     3481 accaaaacag cacgacacag gaaggcggtt ggtcgctcgc gtcattaact aacttgctta
     3541 gcagcgggaa ccaggcctta agcgcagata acatgaacaa cgccgcaggc attctgcaat
     3601 actgcgcgaa gcaaaagctg gcgtcggtaa ccgatgccga aaacatcaag aaccaggtgc
     3661 tggaaaagct gggcctgaac agtgaagagc aaaaagaaga caccaactat ctggatggca
     3721 ttcagggttt gctgaaaacc aaagatggtc agcaactcaa tctggataac atcggaacga
     3781 ctccgctggc agaaaaggtg aaaaccaaag cctgcgatct ggtgttaaag caggggctga
     3841 acttcatttc ctgaaccgcg aaacgccgcg ttttagctgt tacccggctg acgcggctct
     3901 ctcctgccct tttccctgct cctggacggt tttccccctg ttacacggga agcgaccgcg
     3961 atcaaaaaag tctgtttata aactaattcc taacaacatc gcattttttt gacactagaa
     4021 ttgcagcaat atggcgacgc cattacattg tgcttctcaa aaagcttaaa cgttggctaa
     4081 acggcccggt ttagtgagga tcgcccgttg tcagaactac tctctttcgc cctttttctc
     4141 gcctctgtgc tgatttacgc atggaaagcg ggacgtaaca cctggtggtt tgcagccaca
     4201 ttaacggtgc tggggctatt tgtcgtttta aatatcaccc tgtttgccag cgactatttt
     4261 actggcgatg gtattaacga cgcggttctc tataccttaa ccaacagcct gaccggtgct
     4321 ggcgtcagca aatacatcct gccgggtatc ggcattgtgc tggggctgac agcggtgttc
     4381 ggtgcgctgg gctggatcct gcgccgtcgt cgccatcatc cgcaccattt tggttacagc
     4441 ctgctggcgc tcttactggc gctgggttcg gtggacgcca gcccggcatt tcgtcagata
     4501 acggaactgg tgaaatccca gtcacgcgat ggcgacccgg actttgcggc ttattataaa
     4561 gagccgtcga aaactatccc tgacccgaaa ctcaacctgg tttatatcta cggcgaaagt
     4621 ctcgagcgga cctattttga taacgaagct ttcccggatc tcacgcctga actgggcgcg
     4681 ttgaaaaatg aaggcctgga tttcagccac acgcagcagc tgccaggaac ggattacacg
     4741 attgcgggca tggtggcttc tcagtgcggc ataccgctgt ttgccccctt tgaaggcaac
     4801 gcctccgcct ctgtctccag cttcttcccg cagaacatct gtctgggcga tatcctgaaa
     4861 aactcgggtt atcagaacta tttcgtgcag ggcgcgaatc tgcgttttgc cggtaaagat
     4921 gtgttcctga agtcgcatgg cttcgaccac ttatacggct cagaagagct gaaaagcgtg
     4981 gtggccgacc cgcactatcg caacgactgg ggattctacg acgataccgt tctcgatgaa
     5041 gcgtggaaaa agtttgaaga gctttcccgc tcaggtcagc gattctcact gtttaccctg
     5101 acagtcgata cccatcaccc ggatggtttt atctcgcgta cctgtaaccg caaaaaatat
     5161 gattttgacg gtaagccgaa ccagtcattc agcgcggtaa gttgcagcca ggagaatatc
     5221 gcggcgttta tcaacaaaat caaagcgtca ccgtggttta aagataccgt catcgtcgtc
     5281 tcttctgacc atttagcgat gaacaacacg gcgtggaaat acctcaataa gcaggatcgc
     5341 aataacctgt tttttgtcat tcgtggcgac aagccgcagc aagagacgct ggcagtgaag
     5401 cgtaacacga tggataacgg cgcgacggtg ctggacattc tcggtggcga taactatctc
     5461 ggacttggtc gtagcagttt atccgggcag tcgatgtcgg aaatcttcct caatatcaaa
     5521 gagaaaacat tggcgtggaa gccggatatc atccgcctgt ggaaattccc taaagagatg
     5581 aaagagttca ccatcgacca gcagaaaaac atgattgcct tctcgggtag ccatttccgt
     5641 ttgccgctgc tgttgcgggt ttcagacaaa cgcgtggaac cgctgccgga aagcgaatac
     5701 tcagcaccgc tgcgtttcca gctggccgat ttcgctccac gcgacaattt cgtctgggtt
     5761 gaccgttgct ataagatggc acaactctgg gctccggaac tggcactctc caccgactgg
     5821 tgtgtctcgc aagggcagct tggcggtcag caaattgttc agcatgttga caaaacaaca
     5881 tggcagggca aaacggcatt taaagatacg gtcatcgaca tggcgcgtta taaaggcaat
     5941 gtcgatacgc tgaaaattgt tgataacgat attcgctaca aagccgacag tttcatcttc
     6001 aacgtcgccg gtgcgccaga agaggtgaaa cagtttagtg ggatttcccg tccagagtcg
     6061 tggggccgct ggtccaacgc gcagctgggc gatgaagtaa aaatcgagta caagcatccg
     6121 ctgccaaaga aatttgacct ggtgattacc gccaaagcat acggcaataa cgccagccgt
     6181 cctattccgg tacgcgtagg caatgaagaa caaacccttg tgctgggcaa tgaagtgacc
     6241 accaccacac tgcatttcga taacccaacc gatgccgaca cactggtaat tgtgccgccg
     6301 gaacctgtct caaccaacga agggaatatc ctcggacact cgccgcgtaa gctcgggatc
     6361 ggcatggtgg aaattaaagt ggtggaacgt gaaggataat tcctgactga tgtgtaggtc
     6421 gcataaggtg cgacctacac accttctgcg gcctacaaaa catcattgag aaacagaaaa
     6481 aaacagcgcc caacacttcc ccggacgctg catggcaact ttaataatct cgccttatca
     6541 gaaagtaatt acacccttaa tcaactcacg attgttaatc acatcgcgct cgtaggtttc
     6601 tgccagcgtg gcgaacggat agcgatgggt taacatcatg tcagcagtga tttttccttc
     6661 cgccatcagt cgaccgactt tagcaaaatc ttccggcgtg gcgttgcggc tacccatcat
     6721 cgtcgtttct tttttatgga attccggatc ggagaactgc aactcacctt taaacaggcc
     6781 gacaaatacc accgtgccgc cgtgacgaat caaattcacg gtgttattca tcgcatgttg
     6841 attacctgtc gcgtcgatca ctttctgcgc cagcgaacca ccaaactgcg cccgtagctg
     6901 cgcgtcaaaa tcttcggctg acgggtccag taaaggtaat tccagacgcg ttgccacatg
     6961 ttcacggcgc gccggactgg tatccgccac caccacctgt gcgccatcgg ctttagcgat
     7021 tgccgccgcg cccagaccga ttggccccgc cccgaccacc agcacctgct cgccgggagc
     7081 aatggctgcg cgacgcaccg catgagcgct aatagcgaaa ggttcaatca atgctgccgc
     7141 ctgcgggtca ataccgtctg cgggcaaaat gttcgccacc ggcacgctca aatactcact
     7201 aaaaccgcca tcctgatgca cgccaatgac tgaaattttt tcacagcaat tggtacgccc
     7261 gcttttacac gccgggcatt gctgacaggc aacataaggg atcacagcaa cttgctgacc
     7321 atttttaaga tcagcaatat ttttacccag cccaacaatc tccccacata tttcatggcc
     7381 taaaacacgt ggataactaa aaaatggttg atttccaccc caggcatgaa tatcggtacc
     7441 gcaaatcccg acagacttaa tttttattaa tgcttcattg tcacccggaa taggtatctc
     7501 gcgttgtttc cagactaatt ctttcggctg ctggcaaatt aaaacattca tcgtagacat
     7561 gattcggctc tccatttttt gttgccttag atatcaatga aaatgaaaaa tggcagcgga
     7621 acggaaaatc tgttttgtga aaacacacgc ataaaaatgt tttaaatcgg gttttaatgg
     7681 actcacaagg aggaaaatat gagtcgttca caaaatttac gccacaatgt gattaaccag
     7741 gtcattgatg atatggcgcg gggtcatatc ccttccccac ttccatcgca aagtgcgctg
     7801 gcggaaatgt acaacatcag ccgcaccaca gtgcgtcata ttctcagcca cttacgcgaa
     7861 tgcggcgtct tgacgcaggt tggaaacgac tatgttattg cgcgtaaacc tgaccatgat
     7921 gacggtttcg cctgtactac cgcttcaatg agtgaacaaa acaaagtgtt tgagcaggct
     7981 tttttcacga tgataaacca gcgccagtta cgcccagggg aaacgttttc tgaactgcaa
     8041 ctggctcggg ccgcgggtgt cagcccggtt gtagtaagag aatatctttt aaaattcggc
     8101 cgttataatc ttattcacag cgaaaagcgc ggccagtgga gcatgaagca attcgatcaa
     8161 tcctacgccg agcagctgtt tgagctacgc gaaatgctcg aaacacattc acttcagcac
     8221 tttctcaacc tgcctgatca tgatccccgc tggctgcagg cgaaaaccat gctcgaacgt
     8281 catcggctac tgcgtgacaa catcggcaat agctttcgca tgttctcgca acttgacaga
     8341 gattttcact ccttactgct ttcagctgcc gacaatatct tttttgatca atcgcttgag
     8401 atcatctcag tcatctttca ttttcactat caatgggacg aaagcgatct taaacagcgc
     8461 aatatcattg ccgtcgatga gcatatgacc attctcagcg ctctgatttg ccgcagtgac
     8521 cttgatgcca cccttgcgct ccgtaaccat ttgaattcag ctaaacaatc aatgattcga
     8581 tccattaatg agaacacgcg ttatgctcat taagtacgga ttaaaaacag caaactatta
     8641 gcgggtgcac ggttttatca aaagataaat accgggaaat tcccgcttac ctatgctcac
     8701 aatcagtcga ctaaaaatgt ttttgtgagc ggtagtaaag tcctaaaact ttaacctgcc
     8761 gttaatgtca accttgcgta attcacaatt acgtaaacaa ttaaaattat aaatacagga
     8821 gtccggcgtg gaaaaagaaa atatcaccct cgatccgcgt tcttcattta ctccatcttc
     8881 gtcggcagat attcccgtgc caccagatgg attagttcaa cgcagtaccc gaattaaacg
     8941 cattcaaacc accgccatgt tgttattatt ttttgcggcg gtaatcaatt atctcgaccg
     9001 cagttcgctg tcggtagcaa atttaacgat tcgtgaagaa ttgggattaa gtgccaccga
     9061 aatcggcgct ttgctctccg tgttttcact cgcttacggg attgcgcaac ttccttgcgg
     9121 cccactattg gatcgtaaag gcccacgcct gatgctggga ctggggatgt tcttctggtc
     9181 actgttccag gcaatgtctg gcatggtgca caactttacg cagttcgtgt tggtgcgtat
     9241 cggtatgggg attggtgaag cgccgatgaa cccatgcggt gtaaaagtca ttaacgactg
     9301 gttcaacatc aaagagcgcg gacgcccgat gggcttcttc aacgcagctt ctaccattgg
     9361 cgttgccgta agcccaccga ttctggcggc gatgatgctg gtgatgggct ggcgcgggat
     9421 gtttattacc attggtgtac tggggatttt tctcgccatc ggctggtata tgctctatcg
     9481 caaccgcgag cacgtagaac tgactgccgt tgaacaagct tatctcaatg caggtagcgt
     9541 caatgcccgc cgagatccgc tcagttttgc cgaatggcgc agcctgttcc gtaaccgtac
     9601 aatgtgggga atgatgctcg gattcagtgg catcaactac actgcgtggc tgtatctggc
     9661 ctggcttcct ggttacctgc aaacagccta taacctggat ttaaaaagca cagggttgat
     9721 ggcggctatc cctttcctgt ttggggctgc cgggatgctg gtcaacggtt acgttactga
     9781 ctggctggtc aaagggggaa tggctccgat taaaagccgt aagatctgca ttattgccgg
     9841 gatgttctgt tctgccgcct ttacgctggt cgtaccgcaa gcgacaacat ccatgacagc
     9901 ggttctgctg attggcatgg cactgttctg tattcaacat cctgctgggg cttgatccac
     9961 gtcgcagttg cttctcgcat gactgcgtcg gtgggcagta tccagaactt tgccagcttc
    10021 atctgcgcct cttttgcgcc gatcattact ggttttattg ttgataccac ccactcattc
    10081 cgtctggcac taatcatctg cggttgcgtc accgcagcgg gggcactggc gtacatcttc
    10141 ctggttcgtc agccgatcaa cgacccacgt aaagattaat ctccttatgc ccgataacat
    10201 tttgcttatc gggcattttc atggcgatta aaatgtttcc cagttctcct cgctatctgc
    10261 cacggccatt ttacgcggcg cagctggcgt cacggttttt accacagccg atgtttcacg
    10321 ctgctgttgc tgaatccgga acactgccac tgcttcggtc aggcgactgg cctgctcttc
    10381 cagcgcggcg gcggcagcgg cagactcttc caccagcgcg gcgttctgtt gagttacccg
    10441 gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct catcagaagc
    10501 agaagcaatt tcgcccataa tgtccgtcac gcgggtcacg gcgctgacaa tctccgccat
    10561 tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca ccgagtcttc
    10621 aatcaggctt ttaatttcac gagccgcctg ggcgctgcgc tgggccagat tacgcacttc
    10681 tcccgcgacc accgcaaaac cgcgcccttg ctcacccgca cgcgcagcct caaccgccgc
    10741 gttcaaagcc agaatattgg tctggaaggc aatgccgtca attacgctga taatatcggc
    10801 gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat ctaccacttt
    10861 gccgccgcgt tgcgccgttt cagaagcact taacgccaga tggctggcct ggcgcgcatt
    10921 ttcggcgttc tgcttcaccg ttgcggtcag ttactccatg ctggctgccg tctcttccag
    10981 cgaagcggcc tgttgctcgg tgcgcgaaga gagatcgtta ttgccggtgg cgatttcgct
    11041 ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac gcatcagctc
    11101 tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc catccacctc
    11161 aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca ggcgattcat
    11221 tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg ccagtacgac
    11281 gatcatcacg cccaccagaa tccacatcgc ctggctgtag gaggcattgt tatcgctgac
    11341 ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct tctcgaaacc
    11401 gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg cacctaacag
    11461 ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga tctctgccgc
    11521 tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc agtttttttc
    11581 cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg aaccgctacc
    11641 aatattgttc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt tacgcgtctg
    11701 caacaacgcg acccagctgc cattcagcgt ggattgctgc tggcgaatgg tttgtaaaac
    11761 agtgaaattt tctttgtcat tctttaaggc attaaagaac agaccgcctg atgtcagttg
    11821 taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga tacgttttaa
    11881 catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc aaaaacttta
    11941 tgaaaaggtg taacccacca tccatggaaa aaccaggcgc tggttatcag atactaaaaa
    12001 gttattcatt atcgcccggg atggcttctt gtcgggaatt gcccagagca ataaattcct
    12061 ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca atctgccgat
    12121 aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct ttggttagcg
    12181 agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa ccgtcgcgtt
    12241 ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga tacgccagat
    12301 cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac tgttgctcag
    12361 tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct cgcgcctgga
    12421 gcaacgcaat ggttagtgag tcgtgcatta tctttcccct gaaaaattca aaatcattaa
    12481 tatagaaaca gttaatcgtc acgttaccgg gactgcgctg cccctgcaat tcctgatttt
    12541 atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta tctttaaatg
    12601 taaatagttt gttaattaga tcacatttac atcacttact tttgccaaac tacgcaatcg
    12661 ggaataaaag caatcattaa catattaatg attaagatga tcgaatccga ggagtggtaa
    12721 tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat gcatggcagg
    12781 aagcgttcca gcaatccccc tacaaagccc cgcctaaaac tgcggtctgg tttattaaac
    12841 cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt gaaaaggtac
    12901 tgagcggtgc gactgttgcg ctgattgtgg gaaaaacggc gacgaaagta cgtgaagaag
    12961 atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg ccggaagaga
    13021 gcttttaccg cccggcaatc aaagcaaaat gtcgtgatgg attctgcccc attggcgaaa
    13081 ccgtggctct cagcaatgtc gataatctga ccatctatac cgagatcaac gggcgtcctg
    13141 ccgatcactg gaataccgcc gatttacaac gtaacgccgc gcagttgctg agcgccctga
    13201 gcgaatttgc cacgctgaat ccaggcgacg ccattctgct cggcacgcca caggcgcgcg
    13261 tggaaataca gccaggcgat cgcgttcgtg ttctcgcaga aggtttcccg ccgctggaaa
    13321 atccggtagt ggacgaacgt gaagtgacca cgcacaagag cttcccaacg cagccacacc
    13381 cgcacggcac gctgtttgcc ctcggcctga actacgccga ccacgccagc gaactggaat
    13441 ttaagccacc ggaagaaccg ctggtgttcc tgaaagcgcc aaataccctc actggcgata
    13501 accagacctc cgtgcgtcca aacaatattg aatacatgca ctatgaagcg gagctggtgg
    13561 tagttattgg caaacaggcg cgtaacgtca gcgaagccga tgccatggat tatgtcgcgg
    13621 gctacaccgt gtgtaacgac tacgccattc gtgactatct ggaaaactac taccgcccta
    13681 acctgcgggt aaaaagccgc gacggactga cgccgatgct ttcaaccatc gtgccgaaag
    13741 aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc gagttacgcc
    13801 agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc tacttaagtg
    13861 aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacacccaaa ggcttatctg
    13921 acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc ctggtgaacc
    13981 gaattgtgag tgaggataca gcgaaatgaa aaaagtaaat cattggatca acggcaaaaa
    14041 tgttgcaggt aacgactact tccagaccac caatccggca acgggtgaag tgctggcgga
    14101 tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga aagaggcgtt
    14161 cccgaaatgg gccaatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc gtctgggcga
    14221 tctgatcgac cagaacgtgc cagagatcgc cgcgatggaa accgcggaca ccggcctgcc
    14281 gatccatcag accaaaaatg tgttgatccc acgcgcttcc cacaactttg aatttttcgc
    14341 ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga tgctcaacta
    14401 cacgctggtg cagccggtgg gcgtttgtgc gctggtatcg ccgtggaacg taccgtttat
    14461 gaccgccaca tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg tactgaaaat
    14521 gtcggaactc tccccgctga ccgctgacag cctgggtgag ctggcgctgg aagccgatat
    14581 tccggcaggc gtgctgaacg tggtacaggg ctacggcgca accgcagggg atgcgctggt
    14641 tcgtcatcat gacgtacgtg ccgtgtcgtt caccggcggt acggccaccg ggcgcaacat
    14701 catgaaaaac gccgggctga aaaaatactc catggaactg ggcggtaaat cgccggtgct
    14761 gatttttgaa gatgccgata ttgaacgcgc gctggacgcc gccctgttca ccatcttctc
    14821 gatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcagcaaa gcatctaccc
    14881 ggaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtgggcg atccgaacga
    14941 tccgaatacc caggttggcg cgcttattag ccagcagcac tgggaaaaag tctccggcta
    15001 tatccgtctc ggcattgaag aaggcgcaac cctgctggcg ggcggcccgg ataaaccgtc
    15061 cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaaccgtgc tggcagacgt
    15121 tgataaccgt atgcgagtcg cccaggaaga gattttcggg ccggtcgcct gcctgctgcc
    15181 gtttaaagac gaagccgaag gcttacgtct ggcaaacgac gtggagtacg gcctcgcgtc
    15241 gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca ttgaagctgg
    15301 catggtgttc gtcaacaccc agaacgtgcg tgacctgcgc cagccatttg gcggcgtaaa
    15361 agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg cggaaatgaa
    15421 gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct gatatgggta
    15481 agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatcactct gaactgccag
    15541 ggaaaaacca cggttgccgc cagggcgcga tcgacgggca taaagagatc agcaagcgtt
    15601 gccgggaaat gggcgtcgat accattatcg ttttcgatac ccactggctg gtcaacagtg
    15661 cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac gagctgccgc
    15721 attttatccg cgatatgacc tacaactacg agggcaaccc ggagttgggg cagcttattg
    15781 ccgatgaagc cttaaagctc ggcgtgcggg caaaagcgca caacattccc agcctgaaac
    15841 tggaatacgg cacgctggtg ccgatgcgct acatgaatga agacaagcac ttcaaagtgg
    15901 tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag ctgggcgaag
    15961 cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc agcggttcgt
    16021 tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc tacacccgcg
    16081 agttcgaccg ccagatggac gagcgcgtgg tgaagttgtg gcgcgaaggc cagttcaaag
    16141 agttctgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc aatatgcacg
    16201 acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag gtggagttta
    16261 tcaccgagct attcccaagc tctggcaccg gtcaggttaa cgctgttttc ccgcttcccg
    16321 cgtaaggacg ttttatgccg cactttatcg ttaaatgcag tgacaacatc cgcgaagaag
    16381 ccgacctgcc ggggttgttc gccaaagtga atccgacgct ggcagccacg ggtatttttc
    16441 cgctggcggg tattcgcagc cgcgtgcatt gggtcgatac ctggcagatg gccgacgggc
    16501 agcgtgatta tgccttcgtg catatgacgt tgaaaatcgg cgcaggtcgc agcctggaaa
    16561 gccgccagca ggcgggtgaa atgctgtttg aactgattaa aacgcacttc gccgccctga
    16621 tggagagccg cctgctggcg ttgtcgtttg agattgaaga gctgcatccg acgctgaatt
    16681 ttaaacaaaa caacgtgcac gcattgttta agtagcgcgc agattgcccg gtggcgctgc
    16741 gcttaccggg cctacaaaac cccaaaccgt acaccgtagg ccggataagg cgcagccgca
    16801 tccggcaatg ccacaggata tcgctatgtt cgataaacac acccacaccc tgatcgccca
    16861 gcgtctggat caggcagaaa aacagcgcga acagatccgc gcgatctcgc tggattaccc
    16921 ggagatcacc atcgaagacg cttacgcggt gcagcgtgaa tgggttcgcc tgaaaatcgc
    16981 cgaaggtcgc acgctgaaag gccacaaaat cggcctgacc tcgaaagcga tgcaggcaag
    17041 ctcgcagatc agcgaaccgg attacggcgc gctgctggat gacatgttct tccacgatgg
    17101 cagcgatatc ccgaccgatc gctttatcgt gccgcgcatt gaagtggagc tggctttcgt
    17161 gctggcaaaa ccgctgcgtg ggccaaactg cacgctgttc gacgtctaca acgccacgga
    17221 ctacgtgatc ccggcgctgg agctgatcga cgctcgctgc cacaacatcg atccggaaac
    17281 ccagcgcccg cgcaaagtgt tcgacaccat ttctgataac gccgccaacg ccggggtgat
    17341 cctcggcggt cgtccgatca aaccagacga actggatctg cgttggatct ccgccctgat
    17401 gtatcgcaat ggcgtgattg aagaaaccgg cgtcgccgct ggcgtgctga atcatccggc
    17461 aaacggcgtg gcctggctgg cgaacaaact cgcaccgtat gacgtacaac tggaagccgg
    17521 gcaaatcatt ctcggtggtt cgttcacccg cccggttccg gcgcgtaagg gcgacacctt
    17581 ccacgtcgat tacggcaaca tgggttccat tagctgccgc tttgtttaag gagagaacga
    17641 tggaaaacag ctttaaagcg gcgctgaaag caggccgtcc gcagatcgga ttatggctgg
    17701 ggctgagtag cagctacagc gcagagttac tggccggagc aggattcgac tggttgttga
    17761 tcgacggtga gcacacgccg aataacgtgc aaaccgtgct cacccagcta caggcgattg
    17821 cgccctaccc cagccagccg gtagtacgtc cgtcgtggaa cgatccggtg caaatcaaac
    17881 aactgctgga cgtcggcaca caaaccttgc tggtgccgat ggtacaaaac gctgacgaag
    17941 cccgtgaagc ggtacgcgcc acccgttatc cccccgccgg tattcgcggt gtgggcagtg
    18001 cgctggcccg cgcctcgcgc tggaatcgta ttcctgatta cctgcaaaaa gccaacgatc
    18061 aaatgtgcgt gctggtgcag atcgaaacgc gtgaggcaat gaagaactta ccgcagattc
    18121 tggacgtgga aggcgtcgac ggcgtgttta tcggcccggc ggatctgagc gccgatatgg
    18181 gttatgccgg taatccgcag cacccggaag tacaggccgc cattgagcag gcgatcgtgc
    18241 agatccgcga agcgggcaaa gcgccgggga tcctgatcgc caatgagcaa ctggcaaaac
    18301 gctatctgga actgggcgcg ctgtttgtcg ccgtcggcgt tgacaccacc ctgctcgccc
    18361 gcgccgccga agcgctggca gcacggtttg gtgcgcaggc caccgccgtg aagcccggcg
    18421 tgtattaatg ccggatgcgc tgcgcttatc cggcctacac tcgcaccaaa ccgtaggccg
    18481 gataaggcgt ttacgccgca tccggcaaaa agccgtacca aatcgcggac cggataaggc
    18541 gttacgccgc atccgccata aaccttgtcg taccctacaa aaatcccatt agaggaagaa
    18601 aaatgagcga cacctcacct gccataccgg agagtatcga tccggcgaat cagcataaag
    18661 cgctgactgc cggacaacag gcggttatta agaagctatt tcgccgcctg agcgtctttc
    18721 tgttcgtgct gtttatcttc tcgttccttg atcgcatcaa catcggcttt gccggactca
    18781 cgatgggacg cgacctcggt ctgagcgcca ccatgtttgg cctcgctacc accctgttct
    18841 atgccgctta tgtcatcttc ggcattccca gcaacattat gctgagtatt gtcggtgcgc
    18901 ggcgctggat cgccaccatc atggtgctct ggggcatcgc ctctactgcc accatgtttg
    18961 ccactggccc caccagcttg tacgtactgc gtatactggt tggcattacc gaagccggct
    19021 ttctgcctgg tattctgctg tatttaacct tctggtttcc ggcctatttc cgcgcccgtg
    19081 ccaacgcctt gtttatggta gcaatgccgg taacgacagc gttgggctcg atcgtttccg
    19141 gctacatttt gtcgctggat ggcgtcatgg cattaaaagg ctggcagtgg ctgtttttgc
    19201 tggaaggctt cccgtcggta ttactcggcg tcatggtgtg gttctggctt gatgactcac
    19261 cggacaaagc taagtggctg acgaaagaag acaaaaaatg cctgcaagag atgatggata
    19321 acgatcgtct gacgctggtt cagccagagg gagccatcag ccaccacgcc atgcaacaac
    19381 gcagtatgtg gcgggagatc ttcactccgg tggtgatgat gtataccctg gcgtatttct
    19441 gcctgaccaa cacacttagt gcgatcagca tctggacacc gcagatcctg caaagtttta
    19501 atcagggcag cagtaatatc accatcggca tgctggccgc cgtaccgcag atttgtacca
    19561 ttctcgggat ggtctactgg agccgtcact cagatcgccg ccaggaacga aggcatcaca
    19621 ccgcccttcc ttatttgttc gctgccgcag gttggttact ggcttcggca actgatcaca
    19681 acatgatcca gatgctgggg atcattatgg cttcgaccgg atcattcagc gcaatggcga
    19741 ttttctggac aacaccggat cagtccatca gcctgcgggc acgagcgatc ggtattgcgg
    19801 tgatcaacgc cactggcaac attggttcag cgttaagtcc gtttatgatc ggctggttga
    19861 aagatttgac cggcagcttt aacagtggat tgtggtttgt tgccgcgctg ctggtgattg
    19921 gtgcggggat tatctgggcg attccaatgc agtcctcccg tccgcgagcg accctgtaag
    19981 gaacgacgat gtgtgaccgt cagattgcca atattgatat cagcaaagag tacgatgaaa
    20041 gcctgggcac ggacgatgtg cattatcagt cgttcgcccg catggcggca ttttttggcc
    20101 gccatatgct gccacatcgc cacgaacagt actttcagat gcatttcctc aatagcggac
    20161 agattgagct acagcttgac gatcatcgct actcggtgga agcgcccctg tttgtcctga
    20221 cgccgccgtc a
//
LOCUS       DAJVRF010000070        20077 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJVRF010000070 DAJVRF010000000
VERSION     DAJVRF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20077
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.070"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            59..568
                     /gene="radC"
                     /locus_tag="OQN13_002310"
                     /pseudo
     CDS             59..568
                     /gene="radC"
                     /locus_tag="OQN13_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            785..1021
                     /gene="rpmB"
                     /locus_tag="OQN13_002311"
     CDS             785..1021
                     /gene="rpmB"
                     /locus_tag="OQN13_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCS1615652.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            1042..1209
                     /gene="rpmG"
                     /locus_tag="OQN13_002312"
     CDS             1042..1209
                     /gene="rpmG"
                     /locus_tag="OQN13_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCS1615653.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1307..2116
                     /gene="mutM"
                     /locus_tag="OQN13_002313"
     CDS             1307..2116
                     /gene="mutM"
                     /locus_tag="OQN13_002313"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCS1615654.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2155..2634)
                     /gene="coaD"
                     /locus_tag="OQN13_002314"
     CDS             complement(2155..2634)
                     /gene="coaD"
                     /locus_tag="OQN13_002314"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCS1615655.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2642..3919)
                     /gene="waaA"
                     /locus_tag="OQN13_002315"
     CDS             complement(2642..3919)
                     /gene="waaA"
                     /locus_tag="OQN13_002315"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCS1615656.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4332..5390
                     /gene="rfaQ"
                     /locus_tag="OQN13_002316"
     CDS             4332..5390
                     /gene="rfaQ"
                     /locus_tag="OQN13_002316"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCS1615657.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5387..6511
                     /locus_tag="OQN13_002317"
     CDS             5387..6511
                     /locus_tag="OQN13_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCS1615658.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6504..7301
                     /gene="rfaP"
                     /locus_tag="OQN13_002318"
     CDS             6504..7301
                     /gene="rfaP"
                     /locus_tag="OQN13_002318"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCS1615659.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7317..8333
                     /gene="waaO"
                     /locus_tag="OQN13_002319"
                     /gene_synonym="rfaI"
     CDS             7317..8333
                     /gene="waaO"
                     /locus_tag="OQN13_002319"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCS1615660.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWKNEPFMRPINSNYARYCAKHNFKQNEPINGIMNYIYYFYL
                     KIIK"
     gene            8350..9345
                     /locus_tag="OQN13_002320"
     CDS             8350..9345
                     /locus_tag="OQN13_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HCS1615661.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNVSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNIKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            9355..10047
                     /gene="rfaY"
                     /locus_tag="OQN13_002321"
     CDS             9355..10047
                     /gene="rfaY"
                     /locus_tag="OQN13_002321"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCS1615662.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIND
                     TKVSLIDTKYCRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            10073..11098
                     /locus_tag="OQN13_002322"
     CDS             10073..11098
                     /locus_tag="OQN13_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HCS1615663.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            11183..12166
                     /locus_tag="OQN13_002323"
     CDS             11183..12166
                     /locus_tag="OQN13_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCS1615664.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFE
                     LLNQLVESNEKELNDCNLSGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            12212..13465
                     /locus_tag="OQN13_002324"
     CDS             12212..13465
                     /locus_tag="OQN13_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCS1615665.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLITPKKTITNNPTLLIFISLCLLGIINIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNNLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQCLLDK"
     gene            complement(13535..14506)
                     /gene="rfaC"
                     /locus_tag="OQN13_002325"
     CDS             complement(13535..14506)
                     /gene="rfaC"
                     /locus_tag="OQN13_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCS1615666.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENVAMI"
     gene            complement(14510..15556)
                     /gene="rfaF"
                     /locus_tag="OQN13_002326"
     CDS             complement(14510..15556)
                     /gene="rfaF"
                     /locus_tag="OQN13_002326"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCS1615667.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(15566..16498)
                     /gene="rfaD"
                     /locus_tag="OQN13_002327"
     CDS             complement(15566..16498)
                     /gene="rfaD"
                     /locus_tag="OQN13_002327"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCS1615668.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            16712..17908
                     /gene="kbl"
                     /locus_tag="OQN13_002328"
     CDS             16712..17908
                     /gene="kbl"
                     /locus_tag="OQN13_002328"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCS1615669.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            17918..18943
                     /gene="tdh"
                     /locus_tag="OQN13_002329"
     CDS             17918..18943
                     /gene="tdh"
                     /locus_tag="OQN13_002329"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCS1615670.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMAEGFDIGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            19182..>20077
                     /gene="waaH"
                     /locus_tag="OQN13_002330"
     CDS             19182..>20077
                     /gene="waaH"
                     /locus_tag="OQN13_002330"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCS1615671.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSG"
BASE COUNT         5422 a         4377 c         4738 g         5540 t
ORIGIN      
        1 ctgcatgaca aagtcatcgg gcattatctg aacataaaac actatcaata agttggagtc
       61 attacctggc ttgttaacct ctgaatatga acaatttagt ggcgttcatg gaattggcgt
      121 agcgaaattt gcccagttaa aggggattgc tgaactggcg cggcgttact acaacgtgcg
      181 gatgcgtgaa gaaagccctt tactcagccc ggagatgacg cgggaatttt tacaaagcca
      241 gctcacgggt gaggagcggg agatctttat ggtgatcttt ctcgactcac aacaccgggt
      301 tataacgcat agccgtcttt tttccggcac gctaaaccat gttgaagtcc atcctcggga
      361 aattatccgg gaagcgataa aaataaacgc ctcggcgctg atccttgcac ataatcaccc
      421 ttcgggttgt gctgaaccca gtaaagcgga taaactcatt actgaacgga taataaagag
      481 ttgtcagttc atggatttac gcgtgctcga ccatatcgtg attgggcgtg gagagtatgt
      541 ttcttttgcc gaacgcggct ggatttaacc cgctatgcgc gatccttcgg gatctttgtc
      601 tgttcgggac ttgagcacat cgctgagtca gcgtatacta cgccaccttt gagaatctcg
      661 ggtttggcat ttgggcctgg caatcgagag ttcacataga actgcgatga ccgggctgta
      721 aagcctgacg aggcgccaat accccatacg aagctcgagc taatttgatt tttggagaat
      781 agacatgtcc cgagtctgcc aagttactgg caagcgtccg gtgaccggta acaaccgttc
      841 ccacgcactg aacgcgacta aacgccgttt cctgccgaac ctgcactctc accgtttctg
      901 ggttgagagc gaaaagcgtt ttgtcaccct gcgcgtatct gctaaaggta tgcgtgtaat
      961 cgataaaaaa ggcatcgata cagttctggc tgaactgcgt gcccgtggcg aaaagtacta
     1021 agtacttaga ggaaataaat catggctaaa ggtattcgtg agaaaatcaa gctggtttct
     1081 tctgctggta ctggtcactt ctataccact acgaagaaca aacgtactaa gccggaaaaa
     1141 ctggaactga aaaaattcga tccagttgtt cgccagcacg tgatctacaa agaagcgaaa
     1201 atcaaataat tctcgctttg atgtaacaaa aaacctcgct ccggcggggt tttttgttat
     1261 ctgcttgccc ccatattgat tgcatctgtt cattcctgga gatgctatgc ctgaattacc
     1321 cgaagttgaa accagccgtc gcggcataga accgcatctc gttggtgcaa ccattcttca
     1381 tgcggtggtg cgcaacggac gcttgcgctg gccggtttca gaagagatct accgtttaag
     1441 cgaccaacca gtgcttagcg tgcagcggcg ggctaaatat ctgctgctgg agctgcctga
     1501 gggctggatt atcattcatt tggggatgtc tggcagcctg cgcatccttc cagaagaact
     1561 tccccctgaa aagcatgacc atgtggattt ggtgatgagc aacggcaaag tgctgcgcta
     1621 caccgatccg cgccgctttg gtgcctggct gtggaccaaa gagctggaag ggcacaacgt
     1681 gctggctcat cttggaccgg agccgcttag cgacgatttc aatggtgagt atctgcatca
     1741 gaagtgcgcg aagaaaaaaa cggcgattaa accgtggctg atggataaca agctggtggt
     1801 aggggtaggg aatatctatg ccagcgaatc actgtttgcg gcggggatcc atccggatcg
     1861 gctggcgtca tcactgtcgc tggcagagtg tgaattgtta gctcgggtga ttaaagcggt
     1921 gttgctgcgt tcgattgagc agggtggtac aacgctgaaa gattttctgc aaagtgatgg
     1981 taaaccgggc tatttcgcgc aggaattgca ggtttacgga cgaaaaggtg agccgtgtcg
     2041 ggtgtgcggt acgccgattg tggcgactaa acatgcgcag cgggcaacgt tttattgtcg
     2101 gcagtgccag aagtaattca tacgcgccgg atggcatacc atccggcata aacgctacgc
     2161 taacttcgcc accagcgcct gatggacatt ctccggcagg aaatgggtga catcgccctg
     2221 atggcgcgcc acctctttca ccaacgatga agagataaac gaccactctt tcgacggcat
     2281 cagaaacaca ctttccagtt ccggcattaa gtggcgattc atatgcgcca gctgcatttc
     2341 atattcaaaa tccgccaccg cacgcaggcc acgaatcagc accgtagcgt gttgattacg
     2401 ggcgaagttc gccatcaaat cactaaaccc aaccacttcc acgttcccca gatgcgcggt
     2461 tgcctgctgt gtcagcgcca cacgctcttc cagggtaaac atcggttttt tactggggct
     2521 ggcggcaatc gccagaataa cgtgatcgaa catttgcgtg gcgcgcgtca cgatatcgat
     2581 atgaccattg gtaatgggat cgaaagtacc cggataaatc gcccgttttt gcataacaac
     2641 ctcaatgcgt tttcggtggc aggtaaggtt ccagcagttg aagcagacgc tgtagcgcgc
     2701 cctggttttg atacagtact tcaacggcat gacggccata gaaactacgg taatcggcgt
     2761 cggtgagtaa agaggaaacc tcttttgcaa gcgtagtggc atcggtaacg gtaatcagcc
     2821 cgcttgcctg ctccagccgc gcgcaaatgt ctttgaaatt aaaagtatgc ggccccatca
     2881 ataccggaat agcgtgtgcg gcagcttcca gaggattatg ccctccacgt tcaaccagtg
     2941 aaccgccaac aaaggcgaga tcggcaatgc cgtacagtaa catcaactcg cccatcgtat
     3001 cgccaaccac aacctgcgtg ctggtggagg ggacttcccc tgaagagcgt gtgatatagc
     3061 ttagtccagc ctggcggaca aggttaatcg catccgggaa gcgttctgga tgacggggta
     3121 ccaggatgag caataaattc gggaattgct gtaataatgc ctgatgtgcg gcgatcacca
     3181 cactctcttc accttcgtga gtgctggtgg caatccatac cgggcggtgt ggtgcccact
     3241 ggcggcgcag cgtcacggct ttagcagcca actgcggcgt tacagaaata tcgaatttca
     3301 ggctaccggt aacggttacc tggttatttt ttgcgcccag cgccacaaaa cgtgcaccat
     3361 cttcttcatt ttgcgcagca atcagcgtaa tacgacgcag taagcggcgg acgaatttac
     3421 ccagtttggc ataacctgcg gccgagcggg cagagagtcg cgcgttagcg atcaccagcg
     3481 gaattttacg tttatgtagc gccgcaatca ggttaggcca tagttcggtt tccataatca
     3541 acaccagttt ggggtcgact ttattcagga aacggttgag cgcatcgggc aggtcatacg
     3601 gcagataaac gtgctgaaca tccttcccga aagccgattg tacgcgctcc gaaccggttg
     3661 gcgtcatggt tgtaacggta atcggtaaat caggataacg atggcgcagc gcgcgcacca
     3721 acgggatcgc cgccagagtt tcaccgacgg agacggagtg cagcataatg ccgcctggtt
     3781 ttagcggatg gcggtaaaaa ccgtaacgtt caccccagcg ttttcgatag gccggagcct
     3841 tacgtccgcg cacccagagc cgtatccaga tcagcggctg aataaggtag agaagggcgg
     3901 tgtaaagcaa ttcgagcata gtaaatagct gacttatgga tgtgctgggg attctatgta
     3961 tttagctgtg gctttaccat tacttttccc gtttttgact taaatagcaa tttacccgcc
     4021 aattcttgct gaacatctat actttttttg tatttttatg cgattcattg atttctgctg
     4081 tacgtgcagg aaggaatcgc agagcaacag tgaaagaata tggttgctaa tgattttatt
     4141 tgattgattt ctttattgaa aatcaacaat gtgcttaaaa agcattcggg tcggattgcc
     4201 gcgaaagaag tgatacacta gcgcgtcaat atcagtgcgg tatcagtatt caggtagctg
     4261 ttgagcctgg ggcggtagcg tgctttttct gcttcacctt aatcggataa tctcaacaaa
     4321 aagagttact tgtggataag ccatttcgaa gaattttgct cattaagatg cgttttcatg
     4381 gggatatgtt attaactact cccgtcatta gttcgctgaa aaaaaattac cctgacgcaa
     4441 aaatcgatgt gctgctttat caggacacca ccccgatcct gtctgaaaat ccagagatta
     4501 acgcgctcta cggcataaaa aataaaaaag caaaagcctc agaaaaaatt gccaactttt
     4561 ttcatctcat caaggtatta cgtgccaata agtatgacct tatcgtcaat cttaccgatc
     4621 aatggatggt tgctatactg gttcgcttat taaatgcccg tgtgaaaatt tcccaggatt
     4681 atcatcatcg gcagtctgct ttttggcgta aaagtttcac ccatttagtg ccgttgcagg
     4741 gtggaaatgt ggtggaaagt aacttatccg tgctgacacc attgggactt gattcgttgg
     4801 tgaagcagac aaccatgagt tacccgcctg caagctggaa acgtatgcgt cgcgaacttg
     4861 atcacgctgg tgttggacaa aattatgtgg ttatccaacc tacggcgcgg caaatcttca
     4921 aatgctggga caacgccaag ttttccgctg tgattgatgc cttacatgct cgtggttatg
     4981 aagtcgttct gacgtccggc ccagataaag acgatctggc ctgcgtcaat gaaattgcgc
     5041 agggatgcca gacgccacca gtaacggcgc tggctggaaa ggtgaccttc ccggaacttg
     5101 gtgcgttaat cgatcatgcg cagctgttta ttggcgttga ttccgcaccg gcgcatattg
     5161 ccgctgcagt taatacgccg ctgatatcgc tgtttggtgc gacagaccat attttctggc
     5221 gtccttggtc aaataacatg attcaattct gggcgggaga ttaccgggaa atgccaacgc
     5281 gcgatcagcg tgaccgaaat gagatgtatc tttcggttat tccggcggca gatgtcattg
     5341 ctgctgtcga taaattactg ccctcctcca cgacaggtac gtcgttatga tcgttgcttt
     5401 ttgtttatat aaatattttc cctttggcgg tttgcagcgc gattttatgc gtattgctca
     5461 gacagtcgcc gcccgaggtc atcatgttcg ggtttatacc cagtcgtggg aaggcgaatg
     5521 ccctgatgta tttgaactga tcaaagtgcc ggttaaatcg cataccaatc acgggcgcaa
     5581 tgcggagtat tttgcctggg tgcaaaaaca tttacgcgaa catcccgtcg ataaagtcgt
     5641 tggattcaac aaaatgccgg ggctggacgt ttattatgcc gctgatgttt gttatgccga
     5701 gaaagtcgcg caggaaaaag gctttttcta tcacctgacg tcacgttatc gccattatgc
     5761 cgcctttgag cgggcaacct tcgaacaggg caagccgaca cagctgctga tgctgacaga
     5821 taagcaaatc gccgatttcc agaaacatta tcagactgaa gcggagcgtt ttcatattct
     5881 gccaccgggg atttatcctg atcgtaaata tagccagcag ccagctaata gccgtgaaat
     5941 cttccgtaag aagaatggaa taaccgaaca acaatattta ttgttgcagg tcggttcaga
     6001 cttcacgcgt aaaggtgtcg atcgttccat tgaagcactt gcttcgttac cggattcgct
     6061 gcgccacaac acattgctat atgttgttgg acaggataag ccgcgaaaat ttgaggcgct
     6121 ggcagaaaaa cgcggcgtgc gcagtaatgt tcacttcttc tcggggcgca acgatgtctc
     6181 ggaattaatg gcggcggcgg atttattact gcatcctgcc taccaggaag cagcgggaat
     6241 tgtgctactg gaagcgatta ctgcaggatt accggtacta acaacagccg tttgtggcta
     6301 tgcgcattat attgtcgacg ccaattgcgg cgaggctatt gctgagccat tccgccagga
     6361 aacattgaat gagattttac gcaaagcgtt aacgcaatct tcattgcgcc aggcctgggc
     6421 ggaaaatgcg cgacattatg ctgatacaca agatttatac agtctgccag agaaagcggc
     6481 ggatatcata acgggtggtc tggatggttg aacttaaaga gccgtttgcc acgttatggc
     6541 gcggtaaaga tccttttgag gaagttaaaa ccttgcaggg tgaggttttt cgtgaactgg
     6601 aaactcgccg cactctgcga tttgaaatgg cgggcaaaag ctattttctc aaatggcatc
     6661 gcggcacgac cctgaaagag ataatcaaaa atttactctc attgcggatg ccagtattag
     6721 gcgcagaccg cgaatggaat gcgattcatc gactgcggga tgtcggcgtt gatactatgt
     6781 atggggtggc atttggcgaa aaaggtatta atccgctgag cagaacctcg tttattatta
     6841 ccgaagatct gaccccaacc atcagtctgg aagattactg tgctgactgg gcgaccaacc
     6901 caccagatgt tcgcgtaaag cgtatgctta ttaagcgtgt cgcgacgatg gtgcgcgata
     6961 tgcatgctgt gggcattaac caccgtgact gttatatctg tcatttcctg ctgcacttgc
     7021 ctttttccgg taaggaagag gagttaaaaa tttcggtaat tgacctgcac cgggcgcagc
     7081 ttcgcacgcg cgttccacgt cgttggcgcg ataaagatct tattgggctt tatttttctt
     7141 cgatgaatat cggcctgact cagcgggata tctggcggtt tatgaaagtg tattttgtcg
     7201 ccccgcttaa agacattctc aagcaggaac aaggactgct gtcgcaagca gaagcaaaag
     7261 ccacaaaaat cagggaaaga acgattcgaa aatcgttgta atttccggag agaaatatga
     7321 gtgcccacta ttttaatcca caagagatga tcaataagac aatcatcttc gatgaaaggc
     7381 cagcggcgtc agtggcatca tcattccatg ttgcttatgg cattgataaa aactttcttt
     7441 ttggttgtgg tgtttcaatc acgtcagttt tgttacataa caacgacgtg agttttgttt
     7501 tccacgtttt tattgatgat atccctgaag ccgatatcca gcgtttagcc caattggcga
     7561 aaagctatcg tacctgtatc cagatccatc tggtaaattg tgaacggctt aaggcattac
     7621 cgacgaccaa aaattggtct attgccatgt atttccgttt tgtaattgca gattacttta
     7681 ttgatcaaca agataagatc ctttacctgg atgctgatat cgcctgtcag ggaaacttaa
     7741 agccgctgat aacaatggat cttgccaata acgttgctgc tgttgttact gaacgcgatg
     7801 ctaactggtg gtcgttacgg ggtcaaagtc tgcagtgtaa tgaacttgaa aagggttact
     7861 ttaattcagg tgtcctgtta attaatacac tagcgtgggc gcaggagtcc gtttctgcta
     7921 aagcgatgtc gatgcttgct gataaagccg tcgtttcccg tttaacctat atggatcaag
     7981 atatacttaa tcttatcctg tcagggaaag ttaaattcat tgatgctaaa tacaatactc
     8041 aatttagttt aaattatgaa ttaaaaaaat catttgtttg tccaattaat gatgaaaccg
     8101 tattaattca ttatgtcggc ccgacaaaac cctggcatta ctgggccggt tatccaagtg
     8161 cgcaaccttt tatcaacgcc aaagaagcat cgccctggaa aaatgaaccg tttatgcggc
     8221 caattaattc aaactatgct cgttattgcg ccaagcataa ttttaaacaa aatgaaccaa
     8281 ttaacgggat aatgaattat atttattatt tttatttaaa gataataaaa tgatattgag
     8341 aaaagttaaa tgaatgaatt tataaaagaa cggttttcgt atttagcaga taataaaaaa
     8401 gaaaacgccc cagaactaaa tgtttcctac ggtatcgata agaatttttt gtatggtgct
     8461 ggcgtttcaa tttcttccgt tttgattaat aattcagata ttaattttgt ctttcatgtt
     8521 ttcactgatt atgtggatga tgattattta aagtcattta atgaaacagc aaaacaattt
     8581 aatacctcaa ttattgtata tttaattgac ccaaaatact ttgctgatct gccgacgtca
     8641 cagttttggt cgtacgcgac atacttcagg gtattgtcct ttgaatatct gagtgaaagt
     8701 atttccacac tgctgtatct ggatgccgat gtcgtttgta aaggaagtct gaagcctctc
     8761 acagaaatta tatttaaaga tgagtttgct gcggtcattc ctgacaatgt tagtactcag
     8821 gcggcatgtg caaaacgcct caacattccc gagatgaatg gacgttattt caatgcaggc
     8881 gttatctatg tcaatattaa aaaatggcat gaagcaaatt tgacaccgta tttactcacg
     8941 cttttacgag gggaaactaa atatggctct cttaaatatt tagatcagga tgcgttgaat
     9001 atcgcattta atatgaataa tatctacctc gcgaaggatt ttgatactat ttataccctg
     9061 aaaaacgaac ttcatgatcg tagtcatcga aagtatcagc aaaccattac cgataaaaca
     9121 gtattgattc actatacagg gataactaaa ccatggcata gctgggctgg ttatccgtct
     9181 gcatcatact ttaatatcgc gcgtgaacaa tctccctgga agaaatatcc tcttaaagag
     9241 gcgcggactg ttgcagaaat gcagaaacaa tataagcatc tgtttgccca tggtgagtat
     9301 attaaaggca taacttcatt aattaagtac aagcttaaga aataaattac tcttatgatt
     9361 acaagtatac gctatcgcgg cttctcattt tattacaaag ataatgataa taaatataaa
     9421 gaaatctttg atgaaatact ggcctataat tttaaaacag taaaagtatt acgtaatatt
     9481 aatgatacca aagtatcatt gattgatacg aaatattgtc gctatgtttt taaagtcttt
     9541 gccccgaaaa cgaaaaggaa tgaacgtttt ttaaaatctt ttgtaaaagg tgactactat
     9601 cagaatttga tcgttgaaac cgatcgtgtc agaagtgcag gtcttacttt tcctaatgat
     9661 ttttattttc tggctgagcg taaaattttc aactatgcca gtgtttttat catgcttatc
     9721 gagtatgtcg agggcgtaga gctcaatgat atgccgatca ttccagaaga tgttaaagca
     9781 gaaattaagg cttcaatgga aaaattgcat gccttaaata tgctatcggg cgacccacac
     9841 cggggtaact ttatcgttag taaagatggt gtcaggataa ttgacctatc cggtaaaagc
     9901 tgtacggcag agcgtaaagc tcgtgatcgt ctggccatgg aacgtcacct gggtattgcc
     9961 aacgaaataa aagattatgg ctactattca gtaatctaca gaaccaaact acgcaaattt
    10021 attaaaaaat taaaaggcaa agcgtaaacc acacagtcaa aacggaacca acatggattt
    10081 attagctgag agtattactg aagtcgctgt ctctggggaa attgctaaca ccgatcgtgt
    10141 gttaaatatc gcttacggta ttgaccgtaa ctttttattt ggtgcggcag tatctatgca
    10201 atcagttgtt atgcataatc cggaccttgc ggttaagttt catctcttta ccgactacat
    10261 tgatgaagat tatctacaac gtgttaatgc ttttaccagc aaaaatgcta acgttgaagt
    10321 aagaatttat aaagtctcca gtgcctttat tgatatcttc cccagcctga aacagtggtc
    10381 ttatgcaaca ttcttccgtt tagttgcgtt ccagtatctg agtgaaacta ttgaaaatct
    10441 gttatatatc gatgctgatg tcatctgtaa aggctcatta gctggattgc ttgatattaa
    10501 ttttgatggg gataagtttg cagctgttat taaagatgtg ccttttatgc aggaaaaacc
    10561 agcgaagcgt ctggctatag agggacttcc agggaattat ttcaacgccg gtgtagtata
    10621 tctgcagctt gaagcatggg cgaaaaatga ttttatgaat aaagccattg ctatgctggc
    10681 aagtgacccg cagcacacga agtataaatg ccttgatcag gatattttaa atattctgtt
    10741 ctttggtcat tgtattttta ttagcggcga ttatgattgc ttttatggca tcgattatga
    10801 gttaaaaaat aaaagcgatg aagattataa aaagaccatt accgatgata ctaagctgat
    10861 tcattatgtt ggcgtaacga agccctggaa cgactggacg aattatccct gccagaagta
    10921 ttttaatgag gcttatcagg cttcttgctg gaatgatgtg gcgtttattc cagccacgaa
    10981 tgaaaagcag tatcaagtga aatatcaaca tgcaaagaaa aatggtgata cgtttaacgc
    11041 ttttatttac tttattaaat ttaaattaaa taagtataaa agaaaactat ttggataata
    11101 ataaagatca tggatgtctg agttacaata ataattaagg atgagtacta tcatatttta
    11161 ttagttcagg caagagaaca cgatgagcaa tgattaccct ttagtatcca taataatacc
    11221 gacgtataat tcatctgatt acattactga aactctaacg aaattagaaa aacaaactta
    11281 cccaaatttt gaaattgtta ttgttaatga tggttctaaa gataacacat caaacgtttt
    11341 gagagagtat gggttaaccc actctcgatt aattattatc aataaagaaa atggcggtgt
    11401 ttcgtctgcc aggaatacag gtatccgcaa ggcgcaagga cagtttatat gttttatgga
    11461 tgatgatgat gagatagatc ctaactatct gctgaagatg tataccagac aacatgagac
    11521 gggaggggat gcgatttatt gtgggcttta tggccattat ataaaagatg gtgttactta
    11581 ctcacctata aatacagagt ttaatgaagg atctttactt ttcgactttt tttataaaaa
    11641 ggttagattc catatagggt gcttgtttat aagaaaacaa cttctggaag agaataatct
    11701 tttttttgat gaagatttac gactaggaga agatctggat tttatctatc gactgctaat
    11761 tacatgcgat atgtatgcgg ttccatatta tatgtataag cataactata gagaaaattc
    11821 cttaatgaac tcatgtagaa ccatcactca ttatcgacat gagtcatttg cgcacgaaag
    11881 aatctactct tctgtgatgc agttatacaa aggtaacagg aaagaagaaa ttcatacatt
    11941 attgagtcaa aatagagctt atcataaaac tcgttattta tggaatgttc tacttaatgg
    12001 tgattttgaa ctattaaatc aattagttga aagcaatgaa aaagaattga atgattgtaa
    12061 tctttctggc aagagagata aaagacgagc aaaaatatta gcatcaaaaa attatattct
    12121 ctggaggatg gtaagactgg taaataggaa aaagaataaa cgttagatct tgcattaaat
    12181 aatccgtcct cgtagcatag gttgaaacta tatgtcattt tgttggaatg aaattaattc
    12241 tggtataaag tctttaattc tcatattatg tattttttct ttgatgactt tgtctttatg
    12301 ggatgatgtt gcaacaaagt ttcttcatgc agctggaatt atatctgcat tgtattttct
    12361 aataacacca aaaaaaacaa taactaacaa tcctactttg ttaattttca tctcattatg
    12421 tcttttggga atcataaata tcatctggta ttcacattat aaagtttcag gctctgttta
    12481 taccaatgca tatcgtggcc caatggaaac tgggaaaatt gccttgtgta gtgcttttat
    12541 tttcttggtt ctttttgcta aaaatgagtt gagaacaaaa ataaaatttg ggaaactaat
    12601 tctgttcgca tccctggcaa cgcagttact tttttttgcg catgccatgt ggcaacattt
    12661 ctatttaaac gttgaccgtg ttgcattatc agcttcccac gctacaacag caggctacat
    12721 catccttttc ccttctttac tggcatcaat tctcatttta aaatccgact ttagacataa
    12781 aacaacatta tatacaatta acttcatgct tagcttatgt gctgtcatag taactgaaac
    12841 gcgtgcagcc atattagtgt ttccattctt tgcgttacta ttaatcgtaa tggatagtta
    12901 tattaataag cgaattaatt ataagttata ttgttttatt gcgattgcat tattagcagg
    12961 tgtattttct tttaaagata cattgcttat gagaatgaat aacttaaata acgatttagt
    13021 taattattcg catgataaca ccagaacttc agtcggtgcc cgtctggcaa tgtatgaagt
    13081 tggcttaaaa acatattctc caataggaca atcactggaa aaacgagcgg aaaaaataca
    13141 tgaactagaa gaaaaagagc ctagattgag tggcgcttta ccctatatag attctcattt
    13201 gcataacgat ctcatagata cgttatcaac gcgtggtatt cctggagttg tattaacaat
    13261 tttagccttt tcagcaatac tcatatatgc cttaagaact gctaaagaac cttatatttt
    13321 aatcttgctt ttttcactac tggtagtagg cctaagtgat gtaatactct tttctaaacc
    13381 ggttccgact gctgtgtttg tcaccataat attgctttgt gcttatttta aagcacaatc
    13441 agaccaatgt ttattagata aataatgaat atgtgaaata aaatcagcaa ggaggctgat
    13501 tttatttcaa aattatctat taaggggttg taatttaaat catagcaacg ttttcttcaa
    13561 tgaatcgctt aacagcattt gctgtcaatt gagacaactc attccccgga gccctacaaa
    13621 ccatctgatt cttcccatat ccaccaatta atcccggatc ggttggtcca taaaccgtga
    13681 tattgggtct atccagtgcc gccgttaaat ggcttaaccc cgtatccacc gacactacaa
    13741 atttagcccc ggccagcacg cgggcaacgc cttccagact catcttcggc aatacttcaa
    13801 cataagcaaa tccttccgcc agtcgtttcg cccgctcttc ctcatgcggc gcgccccacg
    13861 gaagtttaat ccgtattcct gaatcagcca gtaaaccaat caattctcgc cagtgttctt
    13921 ccggccagtg tttatcatca cgggtcgtcg catgaagaaa tacggcatat tcgccagcat
    13981 ctgtaggcag gttcgtcagg aaatgctgtg caatagcata atcgccctgg gtttgcggtt
    14041 tgctatagcc cagacttttg gcaaacagtt cgcgggtgcg ttctacggcg tgctgctgtt
    14101 ttgcaatatg atgcttacga ttgtaaaaca gactggctaa aggttcgcga gcggtttgcc
    14161 agtccatgcc atgctttacg ccatgcgcca gacgcgtcac cagcgccgcg ctttttacca
    14221 gcccctgagc gtcgataact gcgtcatagt tctctgcttg tagcgcttca cgaaacgctt
    14281 tgcgttccgc ttttatgggg gccgagaacc aggctttacg ccagcgacgt attgccacag
    14341 gaataactcg ctcaacggcg gcgtgccagg aaggaatctg tgcgaaccct tcttccacca
    14401 cccagtcaaa cttaatccct gggattgcct gctgggcatc agtgagtgcg ggcaacgtat
    14461 ggagaacatc gcccatcgac gatgttttaa cgatcaaaac ccgcatccgt caggcttcct
    14521 cttgtaacaa tagcgcgttg agttcttcca gtacgcgctg gggagtaatg tcgattaagc
    14581 tctggtgata accctccgca gcgtcacctt tacgcacttt gtgatagccg gtaatcaggc
    14641 ggatcacgcg cgctttatgg gatagcggcg gtgtgaagtc cgggctactc ggaccataca
    14701 gggcaaccag cggacgattg agcgccgccg caacgtgcat caggccagaa tcgttagtga
    14761 caatggcttt acaggctgca atcaggataa ccgcttgatc aagctgtgtt tcccccgcca
    14821 ggttccgaca ccatgcctgc tgctcggtat tcaaagcggc aagaatctca ttgcccgctt
    14881 catgatcttt cgccgagcca aacagaacca cctgataacc ttcatcaatc agctgctttg
    14941 ccagttccgc atagtggtag tgtggccagc gttttgccgg accaaactcc gcacccgggc
    15001 aaaaaccaat catcggacgt tctgatgaaa gcgaaaattg attacaggta tatgattttt
    15061 caccttcgct cacctgcaat tgcggccata acaacggttt tggtagatct tgtgcggtac
    15121 gcatcacgcc tttgtcatag gccagcgcga catagcgttc caccattagc ggccaggctt
    15181 ctttatcgag cacgcgtaaa tcgttgagta aaccgtagcg catctcgccg cgccagccgg
    15241 tgcgatgagg aatacccgcg aagaaaggca ctaatgcaga tttgaaggag ttgggtaaga
    15301 cgtaggcgcg gtcgtagcgc ttttcacgca ggctatgacc cagtttgcgg cgttcgccga
    15361 tttccagcgc tccgtgaccg agaggcatag gaatagcttc gttaacttcc ggcatccgcg
    15421 ataataatgg acggcaccat gccggtgcca tcacatcgat tatcgcctgg ggatagcgcg
    15481 cctggagcgt gcgatagaga ctttgcgaca tcatcatgtc gccaacccaa gacgggccga
    15541 tcaccagtat tttcatgcag agctcttatg cgtcgcgatt cagccaggcc atgtattccg
    15601 ttacaccttc agcaacggtt ttgaacggtt tgtcgtaacc cgccgcgcgc agatttgtca
    15661 gatctgcctg agtgaacgcc tggtagcggc ctttcagctt atccgggaac ggaatgtatt
    15721 cgatctggcc tttcttgtga taagccagcg tagcatcagc tacagcctgg aaggattccg
    15781 cacgaccagt accgaggttg aagatgccgg aaacgccatt ttccaggaac cacagattca
    15841 catcagccac gtcgcccaca tagacgaagt cgcgtttgaa gttctcgcta ccttcaaaca
    15901 gcttcggtga ttcaccgttg ttaagctggg tgttgagatg gaaagcgacg ctcgccatgc
    15961 tgcctttatg gccttcacgc ggtccataaa cgttgaaata gcggaagcca acaatctgcg
    16021 agttagcttc aggcaggatt tggcgaacat attcatcaaa caggaatttt gagtaaccgt
    16081 agacgttcaa cggtttttcg tactcgcggg attcaataaa gtcggaggtg cgtccgccgt
    16141 aggtggctgc ggaagaggca tacaggaacg ggatttcgcg ctccagacag tagtgcagca
    16201 gctctttgga gtattgatag ttgttatcca tcatatactt gccatcccac tcggtggtgg
    16261 aagagcacgc gccttcgtgg aaaatcgctt cgacatcgcc gaactcttcg ccagccataa
    16321 tctggatcag gaagtcttcc ttatccatat agtccgcgat atccagatcc accaggttca
    16381 caaacttggt gccgtctttc aggttgtcca ccaccagaat atcggtgatg cctttatcat
    16441 tcagggcctt aacgatgttg ctgccgataa agcccgcgcc gccggtaacg atgatcataa
    16501 ctgtaacctt cgaattatgg agtcagagac aatctcagac acgaatactt ctatcatatc
    16561 actacacggc gttggcttca gccattcgcc aaaagtgaga ttgatggacg ttatttatcc
    16621 tgaattttgc agaagtgtta acgcgtcatc tcgtcgcgac ctataagttt gggtaatatg
    16681 tgctggaatt tgccctgtct ggagaatcgc aatgcgtgga gaattttatc agcagttaac
    16741 caacgatctg gaaaccgcac gggcggaagg gttgtttaaa gaagagcgca ttattacgtc
    16801 tgcgcagcaa gcagatatca ctgtggctga tggaagccac gtcattaact tttgtgccaa
    16861 taactatctc gggctggcga attatccgga tctgattgcg gcggcaaagg cgggaatgga
    16921 ttctcacggt ttcggcatgg cttcggtgcg ttttatttgc ggcactcagg acagccataa
    16981 agagcttgaa caaaaactgg cggcattcct ggggatggaa gatgcgattc tctactcttc
    17041 ctgctttgat gctaacggtg gcctgtttga aacgctgctg ggcgcggaag acgccattat
    17101 ctccgacgca ctgaaccacg cgtctattat tgatggtgtg cgtctgtgta aagctaaacg
    17161 ctatcgctat gccaacaacg atatgcagga gctggaagca cgtctgaaag aagcgcgtga
    17221 agccggtgcg cgtcatgtgc tgattgccac cgatggtgtg ttctcgatgg acggcgtgat
    17281 tgccaacctg aagggcgttt gcgatctcgc agataaatat gatgccctgg tgatggtaga
    17341 cgactcccac gcggtcggtt ttgtcggtga aaatggtcgt ggttcccatg aatactgcga
    17401 tgtgatgggc cgggtcgata ttatcaccgg cacgctcggt aaagcgctgg gcggggcttc
    17461 tggtggttat accgcggcgc gcaaagaagt ggttgagtgg ctgcgccagc gttctcgtcc
    17521 gtacctgttt tccaactcgc tggcaccggc cattgttgcc gcatccatca aagtactgga
    17581 gatggtcgaa gcgggtagcg agctgcgtga ccgtctgtgg gcgaacgcgc gtcagttccg
    17641 tgagcaaatg tcggcggcgg gctttaccct ggcgggagcc gatcacgcca ttattccggt
    17701 catgcttggt gatgcggtag tggcgcagaa atttgcccgt gagctgcaaa aagagggcat
    17761 ttacgtcacc ggtttcttct atccggtcgt tccgaaaggt caggcgcgta ttcgtaccca
    17821 gatgtctgcg gcgcataccc ctgagcaaat tacgcgtgca gtagaagcat tcacgcgtat
    17881 tggtaaacaa ctgggcgtta ttgcctgagg atgtgagatg aaagcgttat ccaaactgaa
    17941 agcggaagag ggcatctgga tgaccgacgt tcctgtaccg gaactcgggc ataacgatct
    18001 gctgattaaa atccgtaaaa cagccatctg cgggactgac gttcacatct ataactggga
    18061 tgagtggtcg caaaaaacca tcccggtgcc gatggtcgtg ggccatgaat atgtcggtga
    18121 agtggtaggt attggtcagg aagtgaaggg cttcaagatt ggcgatcgcg tttctggcga
    18181 aggccatatc acctgcggtc actgtcgcaa ctgtcgtggc ggtcgtaccc atctgtgccg
    18241 caacacgatc ggcgtcggcg ttaaccgccc gggctgcttc gccgaatatc tggtgattcc
    18301 ggcgttcaac gccttcaaaa ttcccgacaa tatctccgac gacctggctt ccatttttga
    18361 tcccttcggt aacgccgtgc ataccgcgct gtcgttcgat ctggttggcg aggatgtgct
    18421 ggtctctggt gcaggcccga ttggtattat ggcagcggcg gtggcgaaac acgttggtgc
    18481 acgcaatgtg gtgatcactg atgttaacga atatcgtctc gaactggcgc gcaaaatggg
    18541 tatcacccgt gcggtcaacg tcgctaaaga aaaccttaat gatgtgatgg cagaactggg
    18601 catggccgaa ggctttgata tcggtctgga aatgtccggt gcgccgccag cgtttcgtac
    18661 catgctcgac accatgaacc acggcggtcg cattgcgatg ctgggtattc caccgtccga
    18721 tatgtctatc gactggacca aagtaatctt taaaggcttg ttcattaaag gtatttacgg
    18781 tcgtgagatg tttgaaacct ggtacaagat ggcggcgctg attcagtcag gtctggatct
    18841 ctcgccgatc attacccatc gtttctctat cgatgatttc cagaagggct ttgacgctat
    18901 gcgttcgggc cagtccggga aagtaattct gagctgggat taacacgaac aagggctggt
    18961 attccagccc ttttgcctga ggataatccg ttaaatatgt aaaatcctgt cagtgtaata
    19021 aagatatcgt aattgcgctg atctcttata tagctgctct cattatctct ctaccctgaa
    19081 gtgactctct cacctgtaaa aataatatct cacaggctta atagtttctt aatacaaagc
    19141 ctgtaaaacg tcaggataac ttcagaggtc gtcggtaatt tatgatgaac agcaccaata
    19201 aacttagtgt tattattccg ttatataatg cgggcgatga tttccgcact tgtatggaat
    19261 ctttaattac gcaaacctgg actgctctgg aaatcattat tattaacgat ggttcaacgg
    19321 ataattctgt tgaaatagca aagcattacg cagaaaacta tccgcacgtt cgtttgttgc
    19381 atcaggcgaa tgctggcgca tcggtggcgc gtaatcgtgg gattgaagtg gcaacgggca
    19441 aatatgtcgc ttttgtcgat gctgacgatg aagtctatcc caccatgtac gaaacgctga
    19501 tgaccatggc gttagaggac gacctcgacg tggcgcagtg caacgctgac tggtgttttc
    19561 gtgaaacggg agaaacctgg aaatccatcc ccagcgatcg ccttcgctca accggcgtat
    19621 taaccggccc ggactggctg cggatggggc tttcttcgcg ccgttggact cacgttgtct
    19681 ggatgggggt ttatcgccgc gatgttattg ttaaaaataa cattaaattt attgccggat
    19741 tacatcatca ggatattgtc tggacaacag aatttatgtt taatgcgctg cgtgcgcgat
    19801 ataccgagca atcgttatat aaatattatc tgcataatac gtcagtgagt cggttgcata
    19861 gacaagggaa caaaaacctt aattatcaac gtcactatat taagattact cgtctgctag
    19921 agaaattaaa tcgaaattat gccgacaaaa ttacgattta tccggaattt catcagcaaa
    19981 taacttacga agcattgcgt gtttgccatg cggtgcgcaa agagccggat attattaccc
    20041 gccaacggat gattgccgag atatttactt ccggtat
//
LOCUS       DAJVRF010000071        19737 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJVRF010000071 DAJVRF010000000
VERSION     DAJVRF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19737
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.071"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..1377)
                     /locus_tag="OQN13_002331"
     CDS             complement(<1..1377)
                     /locus_tag="OQN13_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HCS1615672.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSIGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVPLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAP"
     gene            complement(1699..2277)
                     /gene="mocA"
                     /locus_tag="OQN13_002332"
     CDS             complement(1699..2277)
                     /gene="mocA"
                     /locus_tag="OQN13_002332"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCS1615673.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            2380..3150
                     /gene="yqeC"
                     /locus_tag="OQN13_002333"
     CDS             2380..3150
                     /gene="yqeC"
                     /locus_tag="OQN13_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCS1615674.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            3198..4823
                     /locus_tag="OQN13_002334"
     CDS             3198..4823
                     /locus_tag="OQN13_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCS1615675.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILWVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(4864..5796)
                     /gene="arcC"
                     /locus_tag="OQN13_002335"
     CDS             complement(4864..5796)
                     /gene="arcC"
                     /locus_tag="OQN13_002335"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCS1615676.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFVRSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(5844..7228)
                     /gene="hyuA"
                     /locus_tag="OQN13_002336"
                     /pseudo
     CDS             complement(5844..7228)
                     /gene="hyuA"
                     /locus_tag="OQN13_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
     gene            complement(7281..8492)
                     /locus_tag="OQN13_002337"
     CDS             complement(7281..8492)
                     /locus_tag="OQN13_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HCS1615677.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(8550..9746)
                     /gene="dpaL"
                     /locus_tag="OQN13_002338"
     CDS             complement(8550..9746)
                     /gene="dpaL"
                     /locus_tag="OQN13_002338"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCS1615678.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(9804..10991)
                     /gene="ygeW"
                     /locus_tag="OQN13_002339"
     CDS             complement(9804..10991)
                     /gene="ygeW"
                     /locus_tag="OQN13_002339"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCS1615679.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            11470..13248
                     /locus_tag="OQN13_002340"
     CDS             11470..13248
                     /locus_tag="OQN13_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HCS1615680.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(13288..13767)
                     /gene="xdhC"
                     /locus_tag="OQN13_002341"
     CDS             complement(13288..13767)
                     /gene="xdhC"
                     /locus_tag="OQN13_002341"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCS1615681.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(13764..14642)
                     /gene="xdhB"
                     /locus_tag="OQN13_002342"
     CDS             complement(13764..14642)
                     /gene="xdhB"
                     /locus_tag="OQN13_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCS1615682.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(14653..16950)
                     /gene="xdhA"
                     /locus_tag="OQN13_002343"
     CDS             complement(14653..16950)
                     /gene="xdhA"
                     /locus_tag="OQN13_002343"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCS1615683.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            17365..18120
                     /locus_tag="OQN13_002344"
     CDS             17365..18120
                     /locus_tag="OQN13_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCS1615684.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            18199..18268
                     /locus_tag="OQN13_002345"
     tRNA            18199..18268
                     /locus_tag="OQN13_002345"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18231..18233,aa:Gly,seq:ccc)
     gene            18461..>19737
                     /locus_tag="OQN13_002346"
     CDS             18461..>19737
                     /locus_tag="OQN13_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1615685.1"
                     /translation="MEYFMKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYM
                     STLDQIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQND
                     ALGLYLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDAR
                     LNTSSVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYE
                     RITLQLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAG
                     PYGIKRYEKDNYQSANFWFNDIKTDTDQNSHAK"
BASE COUNT         4888 a         4658 c         5064 g         5127 t
ORIGIN      
        1 gggggcaacg tagcaatcgg tcagcggcag gtcttctgcc acttcaatac gctcttctgg
       61 cttccagtgt ttctgggtgt attccatggt taacgcgtct gctgccagtc tgttcagtcg
      121 ttcgacgtca acatggtcaa gtccccaggc gtcggagcct tccagctcgc gcatgcaggc
      181 acttaagcgc agatagccgc caggtttcag caggtcggtt gccatagtaa tagggcgaat
      241 acctgtatca aaaatatcgc ggatagtcag ctgactggca ccaccggaat aagaaatggg
      301 cagtttgccg tcaaaggcgc gagagagaac tgctgcaaca ttgatggaga gcgggaacag
      361 cgcacggcct gacatataca tctcttcacc aggcagtgcg cctttattgt tgatggtgcc
      421 gagagtgtta gtcagtttta cgccaaagcc gagtgatttt tcttttgcca gtaccatcag
      481 gcgctccagc atttccagtg cttgcgtcag cttgaggtcg tgatcaaatg actcttcttt
      541 taagcctatg taaccgaaac cgcagacatc gaggatctca cgaacacgcg cgtaccccag
      601 taaggtcggg ttaagtttga caaaggtgtt gagccctttt tcttccagca tgtagcggca
      661 aatggcttcg atttcatgcg gaggacagcc gtgcatggtg gagaggggga cgccctgcac
      721 catactggtg gggatgcgag cgggtaaggc ttgcaagttt tcgcgttttt cctgcaatcc
      781 gtggcgggct aaaaatgcgt catcctggag taatttattc agcgtatcgc gatattgagc
      841 gaatttcgga tggtcagatg cgtccatcat attgtcgatg aactgttgca tcggcggttg
      901 cttaatacct tcgaggttgt aaccgatgct catattaaag atgaacgatt taccggaatc
      961 agaaggctgt aacatcgctt cgagaaggtg cagggcaaac caggctttga ggtattcatc
     1021 ccaggcttta agcaaggtaa attcggtaga ccattcggtg ttaaagcatt cgtcttcggc
     1081 atcaatacag ggcttttcca gctccaggcg gtcaagaatt tggacggttt ttagttcgat
     1141 gaagcgtccg ccagtcagcc aggaagtgac aatattttgc gcgagctgcg tgtgcgggcc
     1201 agcggcaggg ccgacgggag tggcacaggt ttcaccgaag acgctaacag ttttaccttt
     1261 tacaggtgag taaaactgtt gctcgggaat accaaagatt gagcgttgtt gttggtattc
     1321 atcaaatatg cgcgtcaaaa gttcctcaaa cggaatggga cgcataatat cccccataac
     1381 cctctcctta gcttatacag atatcattga gtgaccggtg ttgagaaatc cggtggttta
     1441 gggggataga gcaacaatcg caccactctt gccaggtttg gcgggaaata taaaaaaatg
     1501 tgaggtggct cgaaacttaa aggctttttg tgaatgaact cacaggtgcc aaatgagaat
     1561 taaaatcact cctgaaaatt attattgtca gcccatttat cactttttct cttttctcaa
     1621 aatcagtgat tccttatcat tatgataaag agtgctggag agattaaata tttgaacgca
     1681 atcgtttaac tacattttct aaattttagt ataccttttt tgcgctgtaa taaagtcgtc
     1741 cggagtatca atatctaaaa ttatttctgc attttctatt tcgacggaat aatgatctcc
     1801 cataaggagt gcctgacgca tattggtgac attgggtcgc tggattgcct gcatcaggca
     1861 tggttttgac actaaaatcg gatggccggg gatgccatta tggagtggca gtattgcgcc
     1921 atcatttcgt aacgaccaga tttttctaaa aatatcgatg gtgagggttg gcatgtcacc
     1981 gtgggtgaga aaacaatgtt ctgtttgcac tgcgggtact gcggccttga cggatgttag
     2041 taaaccctgc gcataatctg ggttgtgaat aatagtgata ttgctctggt tcgcataacg
     2101 ttcgtgcagt tcattaccac gatagccggt gactaaaata attcggctac aaaactgcaa
     2161 cgcatttttg atacttgtat caagaattgt tccctgttcc caggggaaca tcattttcca
     2221 ttgccccatt cttgatgata atccggcagc ggtaattata cagtcgatgg ctgacatagt
     2281 ggttccatta tttatagggg ctaataacgc ctgataattg cgttacttcc tgatggatta
     2341 agttggggat aatttatccc agagaggtca taaagactcg tgaaaagtat agttgaccca
     2401 tcggcattag tcattgattt aggtgcgcag aaacgtccca ctgtgatttc tgttgttggt
     2461 gctgggggga aaaccagctt gcttttttgg ctggcagaac tgctccaggc tagcggcagg
     2521 cgtgtgttaa tcacgacgac gacacatatg tttatgccaa cgtctcactg gcccgtggtt
     2581 ttctgtcgtg atcccgccat gcttcctcat gcgtctctta catcccccat ttcattttgt
     2641 tttcacagct ggaaagcgaa ccagggaaaa gtgcagggat ttacgccaga agcgattgat
     2701 gcactggtgc aacgaccaga gtgtgacgta attctcattg aagcagatgg ctcgcgtgga
     2761 atgccgttaa aagcgcctga tgagcacgaa ccttgcatac ccaaaagcag ttgctgcgtg
     2821 attgctgtga tgggaggaca tactttgggc gcgaaagtga gcacagaaaa tgtccatcgc
     2881 tggtcgcagt ttgctgatat taccgggtta acacctgatg caaccttgca actgagcgat
     2941 ctcgttgcgc tggttcgcca ccctcagggg gcgtttaaaa acgtaccgca aggttgtcgg
     3001 cgagtctggt tcattaaccg tttttctcaa tgtgagaatg cgattgcaca aagcgagctc
     3061 cttcaaccgc tgcaacaaca cgacgtagag gcaatctggc tgggcgatat acaagaacat
     3121 cctgcaatcg cgcgcagatt tgtgaattag cggcccgggt attccgggtt actgaaagga
     3181 tatccattga ggttggtatg aatattttca cagaggctgc aaaactcgaa gagcaaaatt
     3241 gtccgtttgc gatggcgcaa attgttgata gccgaggctc gactccccgc cattctgcac
     3301 aaatgttagt gcgcgccgat ggttctatcg tcggtacaat tggtggcgga atggttgagc
     3361 ggaaggtgat tgaagagtca cttcaggcat tgcaggaacg taagccgcga ttattccatg
     3421 gacgtatggc tcgtaacggt gcggatgctg tcgggtcaga ttgtggaggt gcaatgtcag
     3481 tatttatcag cgtccatggt atgcgtccac gtctggtgtt gattggcgcg gggcatgtca
     3541 accgggcgat agcccagagt gcggcgctat taggatttga tattgccgtt gccgatattt
     3601 atcgcgaaag cctcaatcct gaactattcc caccatcaac cacgcttctc catgctgagt
     3661 cgtttggtgc ggcagtggaa gaactggata ttcgccctga taattttgtc ctgattgcca
     3721 cgaataatca ggatcgtgaa gccctcgaca aactcattga acagcccatt gcatggttgg
     3781 ggttgctggc aagtcgccgc aaggttcagc ttttcctgcg tcaattgcgt gagaaaggcg
     3841 tggctgaaga acatattgcc cgtttacatg cgcccgttgg ttacaacata ggtgcggaaa
     3901 cgccgcagga gatcgccatc agcgtgctgg cagaaatatt atgggtgaaa aataacgcgc
     3961 cgggtgggct gatgatgaaa ccttctcatc cttccggaca ccagctggtg gtgattcgcg
     4021 gtgcgggaga tatcgccagt ggtgtggcgc tacgtctgta tcatgcgggt tttaaagtga
     4081 tcatgctgga agtggaaaaa ccgacagtga ttcgttgtac cgtggcgttt gcccaggccg
     4141 tgttcgatgg cgaaatgacg gtcgaaggcg tcactgctcg cctggcaacc agctctgcgg
     4201 aagcgatgaa acttaccgaa cgcggattca tccctgtgat ggtagatccc gcctgttcat
     4261 tgcttgatga actgaaaccg ctttgcgtgg tggacgctat tctggcgaaa cagaatttgg
     4321 gaacgcgggc agatatggca ccagtaacaa tcgcgcttgg gccgggcttt actgcaggga
     4381 aggattgtca tgcggtaatt gaaacaaatc gcgggcactg gctcggtcag gtgatttact
     4441 ctggttgtgc gcaggagaat accgatgttc ctggcaatat tatggggcat accacccgac
     4501 gggtgatccg tgctcctgct gcaggcatta tgcgatccaa cgtgaaatta ggcgatctgg
     4561 tgaaagaggg cgatgtgatt gcctggattg gtgagcatga aattaaagca ccgttgacgg
     4621 ggatggtgcg tggcttgttg aacgacggcc tggcagtggt tggtggtttt aaaattggtg
     4681 atatcgatcc tcgtggtgaa accgctgatt tcaccagcgt ttctgataaa gcccgggcga
     4741 ttggcggcgg cgtacttgag gcgttaatga tgttgatgca tcagggcgtg aaagcgacaa
     4801 aagaagtgct ggaagtggct taaatgaaaa tggccggatg gtattaccca cccggcctta
     4861 aaattactga caaatcaccg ttccggtttt gccttcaatc ccttctttcg ctttgctcaa
     4921 tacggtaatc agtgcttcac gacctgcgcg agagcgaaca aacgaggcgg cagcttccac
     4981 tttaggcagc atagaacctt tcgcaaaatg accttcttcg ataaagcgtt cagcatcact
     5041 taatgacagg cgatcgagcc actgttcatt ctctttaccg aagttaatgg ccactttttc
     5101 taccgcagtc agaatgatca gcatatcggc atcaatcatt tctgctaaac gggcgctggc
     5161 ccagtcttta tcgataaccg cgctggcacc acgcagatgg ttaccttcac gaataacagg
     5221 gataccacca ccgccaacgg cgatcaccac ctggcctgca tctaccagag ctttaaccgt
     5281 ttctttttca atgatatcaa ccggttttgg cgaggcaaca acacggcgat agccgcgacc
     5341 agcatcttct ttcagagtgt aaccctgttt tgtcagttgc tctgcttctt gctcggtaaa
     5401 gaacgaaccg atcggcttgg tcgggttgag gaatgccgga tcgttagcgt cgacttcgac
     5461 ctgagtaacc agcgttgcta caggtttatt aatgccccga gaaagcagtt cttctcttaa
     5521 cgcgttttgt agatcgtaac caatataacc ctggcttaag gcaacacaga cagacatcgg
     5581 cagcattggc gagtgtgctt cagtttttgc ggcagcttca aaggcttggt taatcatgcc
     5641 cacctgcggc ccgttaccat gagtgacgat aacttcatgt ccttgggcaa ttaaatcaac
     5701 aattgcctga gaagtaattt ttaccgcttt catttgtccg gccaggtcat cgcccagcgc
     5761 attcccgccc agggcgagaa caattttctt actcattttt cctcactcat cattcttgat
     5821 tgtgtataga tccctgacag tggttagagc acgggaggga caaacggttt gcgtcgcagg
     5881 aaacgacctc gcccggcttt gcctgtaaag gtgccgtcac agaaaatcgt ttcaccacgg
     5941 gataaggttc tgacaatcgc gccctgacag gtaaaaccct cccatggcga gtagtcggcg
     6001 ttgtcgtgga gatggcgatg ctgaatttgt tggctctgac gtgggtcgat aatcaccacg
     6061 tcgccatcgg aaccgggcgc taataatcct ttttgcggcc acaggccaaa cagcctggcg
     6121 ggcattgcgc tggttaattc aacaaagcgt tccggtgtta tacgtcccgt catcacgcca
     6181 ctggaaaaca gtaactgcat gcggttttcc acaccgggta agccatttgg gcagcgactg
     6241 aaatcgcctt tagaaatttg caggcgttga gccatcgaga aggtgcagtg atcggtcgcc
     6301 accacgtcaa tcgcaccatc gctgatgcca caccacagtt tgtcctgctc gcgtacgtta
     6361 cgcagcggtg ggctaagaat gaacttcatg ccatcttctg tatcgtaact gcgttcgtcc
     6421 aacaggagat attgtgggca ggtttcaacc cagactggct ggtgattcgc acgggcaaga
     6481 cgcagataat ccagacctaa gccgttagac aggtgcacga tatagagcgg ggcgttaccg
     6541 gcaatttgtg ccaggttaat catgcgggcg atggcttccg cttcgcattc cagagggcga
     6601 ctcaaggcat gatagcgcgg cgcggttaac cctgcggcga taaattccgc ccgcttgctg
     6661 gcgatagccg catcattttc cgggtgcacg gtggtcagcg cgccggattc atgcaagcgg
     6721 cgtaatgcct gcaaaacctc gtcatcgttg agtttgtatt gataggttaa atagagttta
     6781 aaactgctca gtccttcctc gaccatcatc gggatttcgt cgaggattgc gtgattaatg
     6841 tgctggatca caccgtgaaa gctgtaatcg atgaccgctt tatgggcggc ataaccacga
     6901 taaacctcca gttgatggcg taaccgacag ccgtttgggc caaatcccat atggtcaata
     6961 atggttgttg taccgccaca cgcagctgcg cgggtaccgg taaaaaatca tcacaactgc
     7021 gcgcgatgcc gacatcaata ttgaaatgcg tatggacatc cacgccgcca gggaaaacgt
     7081 aacagccagt ggcatcaatt tcttcatacg ggagctgcgg cgaaatattg ttgcccaact
     7141 ggcgaacaat cccgctttca atcagcaaat cctgtttggc ttgtccatct gcgttaacga
     7201 cagtgccgtt tttgatcaat acgcgcatag caaactccaa aaaccctccg gcgaaccgga
     7261 ggggaataag tgaagagtaa ttactctgtt gccagccagc ttaacgggat tgcagcgtac
     7321 atcgcggcac aggtcaccag gtgagatttc caggtttttt cgttaggtgc atgcgcttca
     7381 ggttctttac ccgggccaaa gccgatcacc ggaatgccgt gacggcccat gatagatacg
     7441 ccgttagttg agaaggtcca cttatcaaca accggcgctt tgccaaacag cccttcgtag
     7501 gcattcacca gtgctttaac ggtgaagtga tcttcttcca ctttccaggt cgggaagtag
     7561 cattcggttg ggtaaaccag gccagtccag gacggacggt cgtagttgta catagaaaca
     7621 accgcgttag ctttctgtac tgcaggcagg gcgcggattt cgtccagcgc gccttcccag
     7681 gtttcgcccc aggtcagacg gcggtcaata gagacggcgc agctatctgc tacagcgcaa
     7741 cggcttgggg atgtgaagaa gatttcagaa acggtgaggg tgcctttgcc gaggaattcg
     7801 tcataaccca gacgttggga gagttcttgt aattcgccaa gaatcggacc cattttgaaa
     7861 atggcgttgt caccgcgttc tggcgcagaa ccgtggcagc taacaccctg aacatcaata
     7921 cgaatttcca tacgaccgcg ctgaccacgg tatatctggc agtcggttgg ttcggtactg
     7981 accacaaatt ccgggcgaat gccggattgt tcaataatgt actgccagca cagaccgtcg
     8041 cagtcttctt cctgcacagt accggtaacc agcagggtat attcatcttc cagaccgagg
     8101 tctttaatga ttttaccggc ataaaccata gatgccatgc cgccttcctg gtcggaagta
     8161 ccgcgaccac cgattagctc atcagtttcc atgccttcgt acggatcgaa gtcccagttt
     8221 ttgatgttgc caatgccgac ggtatcgata tgagcgtcca ttgccaccag acgcgggccg
     8281 tggccgatat aaccgagaac gttgcccatc gggtcgattt caactttatc gaagccgact
     8341 ttttccatct cttctttaat acgatgtact acgcgttttt cgtcgcagct ttcactggga
     8401 atagcaacca tgtcgcgcag gaagcgagtc atgtccgcct ggtaatcttt tgctttttca
     8461 agaatcagtt tgaatggaat attcttagcc attatttttc tccagatttt ttactccccg
     8521 ttttgcaggg agtagttcca atagggtgat taaggtgcta cagcgtgttt gccttcccag
     8581 acaacttcgc ggtagtgctt cacgtcggtg tcgccttcag tgctgataac cagcactacg
     8641 gcatctttgt ccagcgccag tttttccatc aggctttgat gttgcgggtg ataatgaacc
     8701 gctgcgagaa cgcccaaacc gacagcgccg gattcaccgg agatgatgcg cgggtcgttg
     8761 ccgtacggat tacccagcac gcgcatacct aatgcggcaa cgctgtcctg gcaggagatg
     8821 aattgggtgg cgcagttacg taggatttcc cagcccagcg ggttaggttc gccacaggcc
     8881 aggcccgcca tgatggtggc catatcaccg ccaacgttga cgatgtcgcc tttgacgccg
     8941 gagcgataaa tacagtcagc tttgtcaggt tcaacaataa tgctgtgcag attttgcggg
     9001 ctgtagacgt cgaccagata acccagcaca ccaccggcca ttgctccgac accggcttgc
     9061 agcagaacgt gcgtcggggt tacgcccatt tcacgcattt gctcgacggc ttcatctgcc
     9121 agggttgcgt agccttgcat gatccaggtt gggattttgg tgtaaccttc ccatgccgtg
     9181 tcctgtacca cttcccagcc gtgctgctgc gcgtgttgca tggtcaggcg aacggtatcg
     9241 tcatagttca tatccgtgac gatgcactcg gcaccgaggt tcagaatggc gtcaacgcgt
     9301 tcctgagcag aacctttcgg catgtaaatc accgcattct gtccgagttg ctgtgctgcc
     9361 cacgccacac cgcgcccgtg gttgccgtcg gtggtggtcg cgaaagtcat tttttcgccg
     9421 atggcatttt tcaggtgctc aaatgacagc gtttcgatat caagatgata tttttcacac
     9481 aataattgag cgatggcgta cgcaccgcca agcattttga aggcgttcag accgaatcgt
     9541 tttgattcgt ctttgacgag aattttcttc acaccaaaaa ggtttgcgag atcgtccagc
     9601 gcacaaagcg gtgttgggcg ataaccagct attttctggt ggaactggcg cgccagtttg
     9661 gcctggcttt gcgaaaagag cggtgatgtt tcgccgttga aaaatttgtt atcggcgata
     9721 tcaatcttca atgagaaaac ggacatatcc tatccttttg gcctctggaa atagagggcc
     9781 gaccagcaga tcggcccgag gggttatttc acgcgttctt gcgcttcttt cagcagttgc
     9841 tcgagcagtg cacctggttt ggcgtattta cgggacagga tcatcgcggc gatgatataa
     9901 ggcttccagc tggcttcttt gtaggtagcg atacggtatt tttcgaatac gccttcagtc
     9961 acttcacctt ctttgcagga tacgccgctg atatcagctg gcaggcagtg catgtacagg
    10021 gcttcgccat cacgggtcag ttccatcatc tcttcagtac aatgccagtc tttgtgttgc
    10081 gcgttctgtg ccagacactg tttttccagt gcttttaagc cttcgtggtc gttcgcacgc
    10141 agcaattcag tacgctcttc catcactttg taaggtgccc atgacttcgg ataaacgatg
    10201 tctgcgtctt tgaaggcttc ttccatgctg gtgacctgac ggaagctacc accggaggct
    10261 ttagcattgt ttttcgccac ttcaaccaca tccgggatca ggtcgtagcc ttccggatgg
    10321 gccaaggtga catccatacc gaagcgagtc atcagaccga tgatgccttg tggtacagag
    10381 agcggtttgc catagcttgg agagtaggcc caggtcatgg cgattttttt acctttcagg
    10441 ttttccagtg aaccaaagtg ttcacgtaac cacgcgaggt cagccattga ctgagtcggg
    10501 tggtcaatat cgcattgcag gttcactaaa gccggacgct gtggcagtac accctgcttg
    10561 taaccgtcat caagtgcagc gccaacttca cgcatatagg cgttgcctgc gcccagatac
    10621 atatcgtcgc gaataccaat agcgtcggcg cagaaggaga tcatattggc ggtttcacgc
    10681 acggtttcgc cgtgagcgat ttgtgatttg ccttcgtcga gatcttgttg tgcaaggccg
    10741 agcaggttaa gcgcggaagc ataagagaag cgggtacggg tggagttgtc gcggaatacg
    10801 gaaataccta atccactatt aaagactttg gttgagatgt tttcagcacg cagtgctttt
    10861 aatgctgcgg caacgtccag tacttgtttc agttcatctg gcgtctgttc ccacgttaac
    10921 aaaaaatctt tctcgtgaag gtgagaggtc agcgaattga tatccttaat cagctcatta
    10981 acagttttca tcatacagtc ccggtagtgg aatgacaggc aaagtggttt atttcactgt
    11041 gacttgccaa agtgattggt gttttcattg ctatacctgg gactaacaat aagtgtgcca
    11101 gaattatccg cgcggctgca aaagactaat ttgacggata atgtgtgggg ttgttcacaa
    11161 attgatgcca gcttctggtt gtttgtaaaa tacgaaaacg tttggctttt tttatcgcgt
    11221 tatttatttt ctgctaagta tcagaaataa gataaagggg caggctatct ctcttcagtt
    11281 tgataaactc accgccgtaa ttctcatatt gatatagaaa tgtgatatct cttccataat
    11341 tgcattagaa aatgataaaa atcttatgtt tttatcaaag ccaatttcgc ctgagttaat
    11401 atcatcatga tctgtgaacc atcaacgtct tcaatgatcg acttttaagt aagtctggaa
    11461 ggtaaagcta tggagcttgc tactacgcag tcagtattga tgcaaattca accgacaatt
    11521 cagcgttttg ccagaatgct tgccagcgtt ttgcagtttg aggttgagat cgttgatgaa
    11581 aacttgtgtc gcgtcgccgg aacgggcgcg tatgggaagt ttcttggtcg ccagttgagc
    11641 ggcaactcac gcctgctccg ccacgtcctg gaaacgaaaa ctgaaaaagt tgtgacacag
    11701 tctcgcttcg atcccctttg cgaaggttgc gatagtaaag aaaattgccg cgaaaaagca
    11761 tttctgggta cgcctgtcat tttacaggat cgttgtgttg gggtgataag tttgattgcc
    11821 gttacccacg agcaacaaga gcatatcagt gataatttac gcgaattttc tgattatgtt
    11881 cgccatatat ccaccatttt tgtttcgaaa cttctggagg atcaggggcc aggagataac
    11941 atcagtaaaa tattcgcgac catgatcgat aatatggatc agggcgtatt agttgttgat
    12001 gatgaaagtc gggttcagtt tgttaatcag actgccttaa aaacacttgg tgttgtacaa
    12061 aataatatta ttgggaaacc tatccgtttc agaccattaa catttgagag taattttact
    12121 catggacata tgcagcatat tgtttcgtgg gacgataaaa gtgaattaat cattggtcaa
    12181 ttgcataaca ttcagggccg acaattattt ttaatggcat ttcaccaatc gcataccagt
    12241 ttttctgtag caaatgcacc tgatgaacca catattgaac aattggttgg cgagtgccgt
    12301 gttatgcggc aattaaaacg actcattagc cgtattgcac ccagcccatc cagcgttatg
    12361 gtggttggtg aaagcggcac gggtaaagaa gtcgtcgccc gagcaatcca taagttgagc
    12421 ggaagacgga ataaaccctt tattgctatc aactgtgccg cgattccgga gcagcttctg
    12481 gaaagcgaac tgttcggtta tgttaaaggc gcatttactg gtgcttctgc caacggtaaa
    12541 acagggttga ttcaggcggc gaatacgggc acgctgtttc tcgatgaaat aggtgatatg
    12601 ccattaatgt tgcaggctaa attactgcgc gctattgagg cgcgtgaaat tctgccgatt
    12661 ggtgccagta gcccaataca agtcgacatt cgcatcattt ctgcaactaa tcagaatttg
    12721 gcccagttca ttgccgaagg taaattccgc gaagatctct tctaccgact taatgttatc
    12781 ccgataactc tgccaccgct gcgtgaacgt caggaagata ttgaactatt ggtgcattac
    12841 tttttacatc tgcatacccg tcgtctggga tcggtttatc ctggcattgc tcccgatgtc
    12901 gtcgaaatat tgcgtaagca tcgttggccc ggaaacctgc gcgagttaag caatttgatg
    12961 gaatatctgg ttaacgtggt tccttcaggt gaagttatcg acagcacgct attgccgcca
    13021 aatctgctga ataatggcac aacggagcaa agtgatgtaa cagaggtcag tgaggcgcac
    13081 ctgtcactcg atgatgcggg cggcacggcg ctggaggaga tggaaaagca aatgatccgc
    13141 gaggcgcttt cacgtcataa cagcaagaag caagttgctg atgaactggg catcggcatt
    13201 gctacgctct atcgcaagat taagaaatat gagttgttaa acacataacc tcagtgaatc
    13261 cggcctgaat tcaggccgga tatcctttta cttcgttttc tcgcaatcca gaactgtatt
    13321 tacaatcatc tgataccccg tgcagcgaca aagatttccc gccagtccgc gacgaatttc
    13381 cgtaatggtt aatggcttct cgcgtggttt cgccagcatt gccgtggtag ccataatcag
    13441 gccaggcgta caaaacccgc actgcactgc gccggatttc gcataagcct gctgaacatg
    13501 agaaagtttt ccgcctttcg cttcaccttc cagcgtgcgg atctcctttc cttcagccca
    13561 ggcggcaagg tataagcaac tgtctattgc tgtgccgtcg accaacaccg tacaggcacc
    13621 acattcaccc acgcagcacc cttgtttgac acttagcagt ccttgttcgc ggagtaattc
    13681 cgagagcggc gtgcctggtg cggcgtgaag ctgaaaaggc atcccgttaa tggtgcattc
    13741 gatggtaatt gtttcgctgt gattcattgc aattttcccc ccgccgcggc gacggcttcg
    13801 ctaatcactt ttttggtcat cgtctggatg agatgcagac gaaactcttt actggcccgc
    13861 catgaagaac gcggggcgac atcttgcagg acagattcgc tgatagcttc cagcgtttgc
    13921 aggtttaatg gcgcattttg tgcagtctgt tcggcatgtt ggcagcgaat cggcgttggc
    13981 gcggcaacac caaatgccag gcgtaattcg ctgaaattgc cgttatccag tcggcaatgt
    14041 gcggcgcagc caatcgttga aatatccatt gcgtcgcgca tggcatattt aaaatgcgcg
    14101 ctgcccgcgt gttctttcgg ctgtggcgga aaatgaaagg cgacgaggat ttcgtcatgc
    14161 tcaagagaca ctttgcccgg cccggtgtga aagccattaa tcgggacgaa acgaacaccg
    14221 cgcggggagt ggatctccag tttcgcgtca taaattagcg ttggcgtggc agaatctgcg
    14281 ctggtggcac cgttgcaaat atttccaccg taggtagcga cgttacggat ctgcggtcca
    14341 gcaatggacg tggccgcagc acataacgcc gggagatgac gttgagttat aggagcttct
    14401 attagctggg taaatgtcgt tgcagagccg atacgtagcg agccatcttc cgccagcgta
    14461 attccccgca gctccgccag attatgaata tcaacaatat ggcgataacg gtcattgtga
    14521 tggtggagct gaatcagtac gtcagtgcca tcggcgagca gtttggcctg cgggttgtca
    14581 gccagcaggt tgatggcatc ggcaagggtt gctgcgcgat ggtaagaagc aaaatcaaac
    14641 atgatgttat cctcaaatca atcctgccag atggaactct tcatataacc gttttggcgt
    14701 cagcggcagt gtattgattg caacaccggt agccatcttc accgcgttac gaatagcagc
    14761 ggcaacagga attattggtg gctcacccag tgacttatgt ccgtatgcgg attgcggctc
    14821 attgatttcg acgaacgcgc tttccagttg tggcagatcc ggcatggtcg gcattttgta
    14881 atccagcaga ttggggttac ggaccacgcc gcttttagca tcgatgatca tctcttcaaa
    14941 tagcgcccag ccaatgccca ttcccattcc gccgtgtacc tgaccttctg ccagcagtgg
    15001 attaagaata tgccctgaat catgaacgtt gaggatgcgg ttgatggtga ctttgcacag
    15061 cgcaatatcg accgtcagat caacaaaggt acagccaaac gccgatgggt tagtggtggt
    15121 tttgatggag ctttcagcag agagctgccc gccgcgttca gggtggtaga aagcgtccat
    15181 cgccaaatct tttaacgaca ttaacggctc ttccggtcgt tcaaccagca cgatatggcc
    15241 ttttatcagg gtcagattca tcgctgactg atgtagcatg actgcggcgt gagcgatgat
    15301 tttctctttt aataatagtg ccgcactgcg cagcgcaggc gcggcaacat agctctggcg
    15361 tgaggcaaat gcgccgggag cgaacggcgt aacgtcggta tcttgagttg aaataacgcg
    15421 aacgtcgctg accggaaccc ccacggtttc tgccaccatt tgcgagaaga cggtgtcggc
    15481 accctgaccg atttccgtcg cgccgctttg cacgttgatg gttccatcct gattcatcag
    15541 aaggcgcgcg cctgctattt ctacgccgac aggccaggtg ttagaggtat agctaaaaca
    15601 ggcgacgcca acgccgcggc gcaaattgcc ttgctggttc tggcattctg cacggcgttt
    15661 ttcccattca aagattttcc ggcctttttc aagacactcc ggcaaccctg cgctgtaaat
    15721 acgtttgccc gtgagcggat tagcatctcc ttcgcgggcg gcgttgcgta aacgaatttc
    15781 aacaggatca atacctaacg ctgtcgcggc gtcatcaagc atagactcaa cggcaaatac
    15841 gacttgtggc gcgccataac cacgcatcgc accagccgag gggaggttgg tatagcaggt
    15901 ctttgaactg taagcgtagg cacaacgagg ataaaggtaa gcgactttat tcctccccgc
    15961 agaagcgatg gagtgcccgt gagatgcata agcgccggtg ttagacagaa catccagact
    16021 ataacctttc aatgttccgt cgcggttcac gcccatttgc ccgtcaatgg taaaagcgtg
    16081 gcgggtacgg gttgcgagga aacactcttc acggctaagg gaaactttca ccggaatgcc
    16141 gccaagcttg ctggtcagga atgctgccat tggctcttcc agtacatcct gtttattacc
    16201 aaaaccgcca ccgacaaacg gtttgatgac tcgtacgcat gaccagggaa tatccagcgc
    16261 ctgaccaacc acgcggcgaa caatgtgcgg gatctgggtg ctggaaacga tggtaattcg
    16321 cgagtcatcc tccatccacg ccagcgatgt tacgctttcc atgtgacaat gttgaataac
    16381 gggggtctga tagtgcccct gtacctggta gtcggcggca tcgattgttt gttggacatt
    16441 acccgtcgac atcgtgcttt gtttcagtaa attgccaccg ttatggattg gtgcagcgtc
    16501 ttctgccagc gccgcttctg gcgtggtgat aacgggtaat tcttgccact caatgctgac
    16561 caattgcgcc gctttttctg ccgtgagttc atcgcgggcc acgacgatgg caacggcgtc
    16621 accatgatga cgaacatggc gagttagcag tgcgcgatcg gcggtatcgc gcttgttttc
    16681 gtcaagtgtc caggcatgcc cagctgtagc gaatggaata tcaggcacat cttcccaggt
    16741 aaaaatcgcc agtacgcccg gcaaacttct ggcttgttca tcattaatac ttacggcata
    16801 accatgtgcg atagggctac gtacatattt cgcataacac atgcccgcca taacataatc
    16861 gtcagtatat cgtgcccgcc cggtgacctt agcaatggca tcgacgcgca tgcatgattc
    16921 acccgtagcg gttgcttccc gcgcttccat agaaatcccc tcgatttgaa catttggttc
    16981 ttatggcaaa gatttagtct aagcaggcaa gatttacgcc agaaacgacg ataagcgtta
    17041 ggggaggtag gaagtgtgat gacgttaaaa aaacacagat atgatacgaa acaggactct
    17101 gaataggggc agtgatacgt atcaatatga gtggcgataa aatcaatgtg ataaattggg
    17161 attattacgc atcaggatgg gaaatagggt aaaaagtcag gaagttcgat acctctacag
    17221 cgaatatttt gctgatgata cttccctttg ccaaatgaaa ttaacctctt tctctattac
    17281 cacgtttttc cagaagcaag agattgggta tcaagaggct ggctgctatg ataaggcgtc
    17341 ttgtttttta agcgaggaaa gattttgagt gcgggacgcc tgaataaaaa atctctgggt
    17401 atcgtgatgt tgttatcggt tggactgctt ttggcgggct gttcgggtag caaatcatcc
    17461 gatacaggaa cgtattccgg ctccgtttac accgtgaaac ggggggatac gctatatcgt
    17521 atttcgcgca ccacgggaac cagcgtaaaa gaactggcgc gactgaacgg catttccccc
    17581 ccttacacca ttgaagttgg tcagaaacta aaactgggtg gggcgaaaag tagcagtagt
    17641 acacgtaaat caaccgccaa atcaacgacc aaaaccgcat cggttacacc gtcatcagcg
    17701 gtaccgaaat catcctggcc gccagtaggg caacgttgtt ggttatggcc aacgacaggg
    17761 aaagttatca tgccgtattc gacagcagat ggcggcaata aagggattga tatctcagct
    17821 ccacggggta cacctattta cgccgcgggt gcaggaaagg tggtgtatgt gggcaaccag
    17881 ctgcgtggct acggtaatct catcatgatt aaacacagtg aagattacat tacggcttac
    17941 gcccataatg acacgatgct ggtaaataat gggcaaagcg tgaaggctgg gcaaaaaatc
    18001 gccactatgg ggagcacgga tgcggcatct gttcgcctgc atttccagat tcgttaccgt
    18061 gcaacggcaa ttgatccgct acgttacttg ccgccgcagg gcagcaagcc aaaatgctga
    18121 tggcgaatta atcagcagtc agcagcgcgg cacttgctaa ggagagcgta aggtttataa
    18181 tgccttacgc atctcgaagc gggcgtagtt caatggtaga acgagagctt cccaagctct
    18241 atacgagggt tcgattccct tcgccgcttt tgctgtatct gattttctat attatcttaa
    18301 ttcaatcgct tcagactttt cgcaatcgcc gtaatatttc cccattttgt tgtaaataaa
    18361 aagcgtttac gcaatgcaat ccttttgtaa caacccaggt aaaacacctt attttcaagt
    18421 tgttaaataa gtggttattt acgcttttaa caaacgattg atggaatatt ttatgaaagg
    18481 gaaatctgca ttaactcttc tgctcgctgg tatattttcg tgcggcacat gccaggcgac
    18541 aggggcagaa gtgacgagtg aatctgtatt taacattctt aattccacgg gggcagcaac
    18601 agataaaagt tatttatcgt taaatccaga taagtaccct aattatcgtc ttctgattca
    18661 ctcagccaaa ttaaaaaatg aaataaagag ccactatacc aaagacgaaa ttcaagggtt
    18721 gttaacatta acagaaaaca cacgaaagtt aacgttgacg gagaaaccct ggggaacatt
    18781 tattcttgct tctactttcg aagatgacaa aacagcggca gaaacccact atgatgctgt
    18841 ctggctgagg gatagtttat ggggctatat ggcattagta tcagatcagg gaaatagtgt
    18901 agccgcaaaa aaggttctgc tcaccctgtg ggattatatg tctacgctcg accagattaa
    18961 gcggatgcag gacgtcatta gcaaccccaa acgactggat ggagttcccg ggcaaatgaa
    19021 tgctgtgcat atccgatttg acagcaactc ccccgtgatg gcagatgtgc aggaagaagg
    19081 caagccgcag ctgtggaacc ataaacaaaa tgatgcgtta ggtctttatc ttgatctcct
    19141 tatacaagca atcgacactg gtacaattaa cgctgaagat tggcaaaaag gtgaccgatt
    19201 aaaatcggtt gcgctgctga ttgcttatct ggataaagcc aatttctatg ttatggaaga
    19261 ttccggagca tgggaagaag atgcccgatt aaacacatct tccgttgcac tggtgacctc
    19321 tggactggaa cgtttgtcga acttactctc gaagaaagac tctgtttttg tctctgattt
    19381 actacgcgaa gctaaagcca atgaactgga tgaacctctt tcaacaacga ggttgaacca
    19441 cctcatagat aaaggttatg aaagaataac gttacagctt gatttagggg gagaatcccc
    19501 aggttatctg gaaaaagata aacattacag agaagccgat gcggctttac ttaatgttat
    19561 ttatcctgca aatcttgcaa agataaacac cagaagaaaa gagcaggttc taaaaatagt
    19621 taaaaaacta gcaggtccgt atggtataaa aagatacgag aaggataatt atcagtcggc
    19681 aaatttttgg ttcaatgata ttaaaaccga taccgatcaa aattcacatg cgaaaag
//
LOCUS       DAJVRF010000072        19407 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJVRF010000072 DAJVRF010000000
VERSION     DAJVRF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.072"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..245
                     /locus_tag="OQN13_002347"
     CDS             <1..245
                     /locus_tag="OQN13_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCS1615686.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /locus_tag="OQN13_002348"
     CDS             complement(296..1063)
                     /locus_tag="OQN13_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1615687.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /locus_tag="OQN13_002349"
     CDS             1259..2167
                     /locus_tag="OQN13_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS1615688.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /locus_tag="OQN13_002350"
     CDS             2164..3426
                     /locus_tag="OQN13_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCS1615689.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="OQN13_002351"
     CDS             3423..4061
                     /locus_tag="OQN13_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCS1615690.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="OQN13_002352"
     CDS             4066..4842
                     /locus_tag="OQN13_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCS1615691.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGYEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="OQN13_002353"
     CDS             4931..6295
                     /locus_tag="OQN13_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCS1615692.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALCASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="OQN13_002354"
     CDS             complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="OQN13_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCS1615693.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7444..8583)
                     /gene="nlpD"
                     /locus_tag="OQN13_002355"
     CDS             complement(7444..8583)
                     /gene="nlpD"
                     /locus_tag="OQN13_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCS1615694.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="OQN13_002356"
     CDS             complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="OQN13_002356"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCS1615695.1"
                     /translation="MVSRRVQALLEQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9343..10104)
                     /gene="surE"
                     /locus_tag="OQN13_002357"
     CDS             complement(9343..10104)
                     /gene="surE"
                     /locus_tag="OQN13_002357"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCS1615696.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10085..11134)
                     /gene="truD"
                     /locus_tag="OQN13_002358"
     CDS             complement(10085..11134)
                     /gene="truD"
                     /locus_tag="OQN13_002358"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCS1615697.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="OQN13_002359"
     CDS             complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="OQN13_002359"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCS1615698.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="OQN13_002360"
     CDS             complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="OQN13_002360"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCS1615699.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="OQN13_002361"
     CDS             complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="OQN13_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCS1615700.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12844..13167)
                     /locus_tag="OQN13_002362"
     CDS             complement(12844..13167)
                     /locus_tag="OQN13_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCS1615701.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="OQN13_002363"
     CDS             complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="OQN13_002363"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCS1615702.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="OQN13_002364"
     CDS             complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="OQN13_002364"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCS1615703.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="OQN13_002365"
     CDS             complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="OQN13_002365"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCS1615704.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16411..17448
                     /gene="iap"
                     /locus_tag="OQN13_002366"
     CDS             16411..17448
                     /gene="iap"
                     /locus_tag="OQN13_002366"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCS1615705.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(17716..18009)
                     /gene="cas2"
                     /locus_tag="OQN13_002367"
     CDS             complement(17716..18009)
                     /gene="cas2"
                     /locus_tag="OQN13_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCS1615706.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18006..18929)
                     /gene="cas1e"
                     /locus_tag="OQN13_002368"
                     /pseudo
     CDS             complement(18006..18929)
                     /gene="cas1e"
                     /locus_tag="OQN13_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
     gene            complement(18926..>19407)
                     /gene="cas6e"
                     /locus_tag="OQN13_002369"
     CDS             complement(18926..>19407)
                     /gene="cas6e"
                     /locus_tag="OQN13_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCS1615707.1"
                     /translation="FFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTICK
                     AGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQ
                     QLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
BASE COUNT         4592 a         5230 c         4847 g         4738 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggca cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgctct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gtccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt atgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgctcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgtgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgagca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca agttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt
     7201 aaagctgagt cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg
     7261 ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc
     7321 cgttctcatc aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca
     7381 taaggtggct cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgcc
     7441 gatttatcgc tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc
     7501 aaaatgcaag cgtgttgaac tggttccggt gctacccatg gttgctattt tttgccccgc
     7561 cttcacttct tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta
     7621 atcatcatta tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccggcata
     7681 aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat
     7741 ccccttgttg cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca
     7801 gcgccaggtg gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct
     7861 tgctgttggt accgttacag gcgctgtgac tgtggtcgca gttggcttgt tgttcggcaa
     7921 cattttgtta gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc
     7981 aacagcaacg gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc
     8041 ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt
     8101 ctgaccaacg ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa
     8161 atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt
     8221 actgccgcta taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc
     8281 gttttccatc tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg
     8341 agtagcctga atttgtggct gctgtaccgg ctgaatttgc ggttgctggg ctgtagacgt
     8401 cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt
     8461 gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca
     8521 tagcgaaacc agtgacaaag cagcaatgcg gcgaacggtg aattttgggc ttcccgcgct
     8581 catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc
     8641 ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa
     8701 aatccaggta tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca
     8761 cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact
     8821 ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg
     8881 cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct
     8941 gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca
     9001 ggcggcgacg tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga
     9061 caagatgcgc taggattgcc gtttgatatc ccgaaccagt gccaatttcc agcacccgcg
     9121 actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg
     9181 tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat
     9241 caacgaattt ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac
     9301 cttgcgcacg taattgctcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac
     9361 tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac
     9421 atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc
     9481 ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg
     9541 ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt
     9601 gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg
     9661 ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt
     9721 atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc
     9781 ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga
     9841 cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt
     9901 cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga
     9961 ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac
    10021 gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc
    10081 atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc
    10141 tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc
    10201 aattccagct taattgttgc ggatacagca acatcgctct gcgcgcggct tcaacttttt
    10261 cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgccttt tcgaatgcca
    10321 gcgcttcacg ctgagttccc cattcgccac tacccggcaa tgcggcggtt atcatcagct
    10381 ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc ggtggttgcg acaaaccagc
    10441 taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt
    10501 tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac
    10561 tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca
    10621 agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc
    10681 aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg
    10741 taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca
    10801 gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc
    10861 gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt
    10921 cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc
    10981 cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat
    11041 cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac
    11101 cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac
    11161 cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc
    11221 tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg
    11281 tggaatgtgc ggcaacatct tcggtgcctg agcgataata gtgacatcga cgttgcccag
    11341 ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc
    11401 acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc
    11461 gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa
    11521 tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc
    11581 atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg
    11641 aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg
    11701 ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata
    11761 gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat
    11821 tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg
    11881 cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg
    11941 ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg
    12001 gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa
    12061 tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt
    12121 tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc
    12181 gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc
    12241 ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca
    12301 tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca
    12361 gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc
    12421 atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca
    12481 atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc
    12541 gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa
    12601 tactgtagcc agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct
    12661 gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata
    12721 ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc
    12781 agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac
    12841 aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt
    12901 gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac
    12961 taccgcgacg ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt
    13021 gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac
    13081 cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt
    13141 tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg
    13201 gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt
    13261 gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg
    13321 cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat
    13381 ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga
    13441 taaagcgccc ttctcctacg cgttcacgaa ccatctggcg ttcggcgcgg tgtggcgaga
    13501 taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc
    13561 gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc
    13621 gaacattatc gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca
    13681 gcgccccggc gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac
    13741 cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga
    13801 cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg
    13861 aaagtggcga cgaaccagag cattcaattc cagttcgaat gcactgaatt cagatggcgc
    13921 agcagttgcc tggctaactg gctcgtgcac cataccggca cccacggtca cattgctcag
    13981 gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag
    14041 cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc
    14101 acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac gcgcgcgcgt
    14161 cttcttaccg gcaattttga tgtcgtaact ctgccctgga gaaagcggct gttccgccat
    14221 ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg ccgccagcag
    14281 cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc
    14341 aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc
    14401 agagggcagc acttttacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc
    14461 gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg
    14521 agcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct
    14581 gtaccacggc atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg
    14641 cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg
    14701 aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac
    14761 cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac
    14821 gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat
    14881 attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt
    14941 agagaaatag cggtaggcca catcaatagt gatgccctgt tcgcgctcag cttgcaggcc
    15001 gtccaccagc agagccagat ccagcttttc gccctgggta ccgtgacgct tactgtcgtt
    15061 atgcagcgat gagagctgat cttcatagat ttggcgagta tcgtgcagca ggcgaccaat
    15121 cagagtgctt ttgccgtcat cgacgctgcc acaggtcaga aaacgcagca ggcttttatg
    15181 ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc
    15241 ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagatccc gcctggtcgc
    15301 ggtcaatcac gcggccctga cgttcactgg tggtggaaac cagcatctct tcgatgattt
    15361 ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac
    15421 ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat
    15481 caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg
    15541 gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag
    15601 agagtgggaa gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca
    15661 gctccgggcg ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc
    15721 gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac
    15781 cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc
    15841 tgccgtgcac gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca
    15901 gctcgcagcc gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc
    15961 agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg
    16021 ccaaatgcag catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga
    16081 attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag
    16141 taagtcgtat ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat
    16201 gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc
    16261 ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttct
    16321 ggcgtttagg aagatttgaa attgttttag cgcagcggaa gtttcatact atggcggtaa
    16381 aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct
    16441 gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc
    16501 gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga
    16561 actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat
    16621 cgcagtgata ttcggacatt taatagtcgg tatatttata ctgcccgcga taatcgtaag
    16681 agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag
    16741 cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat
    16801 gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc
    16861 atgctggaac tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg
    16921 gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga atttactcaa gcggatgagt
    16981 gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat
    17041 aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac
    17101 agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc gggtttgaat
    17161 aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc
    17221 attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag
    17281 caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat
    17341 aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt
    17401 atgcggataa tgctacctct ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa
    17461 tgggagctgg gtgttctacc gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc
    17521 cagtgcgccc ggtttatccc cgctggcgcg gggaactctt actgcttggt atgcggaatc
    17581 acaccctgaa cggtttatcc ccgctggcgc ggggaacaca ctaagcatac ataactgttt
    17641 ttaaacaaat ttattccgca tcaacaatct accaactaaa ttaaaacatt tccttatttt
    17701 taaagaacgg ataacttatt gattatcaac aggaagaaaa gaaaccaaac gtaacccatc
    17761 caaatccacc ggaatacgtc tgttttctcc ccaggtctga aattcaaaac ccgactcgat
    17821 attggtcgcc caggccatca ccacatttcc gcaaccagcc agttgggtaa tttgctgcca
    17881 gatcatctcc cgaatacgtt ttgatgtatc accaacatac acaccggcac gcacttccag
    17941 tagccagatt gcgagccgtc cacgtaagcg cggagggaca ttttctgtaa caaccacgac
    18001 catgctcatc cgccgcgccc ccggtgacca ctatcaccca gcgtttcagg ttcagggatg
    18061 gcaggcggta acatatccgg cgcgggttgt ggtggttcaa tttcacctgc agcaaggact
    18121 tcctcaatta acggtattaa tttgcccgtt aacttagtgc tacggaaaat atcgcgacag
    18181 gctaatctga cttctttatc aggttctgcg ggttgcctcg ctgctatttc aaatgccttt
    18241 ggcacaaccg aatcaaattt aatgatatcg gctatgtcat aaacaaatga aagcggtttg
    18301 ccactatgga taaacccaat agcgggcgca tatcccgcgg ctaatactgc cgcttcagat
    18361 ataccgtaca gacatgatgt agcagcactg atgcagcgat tcacaacatc gcctttttcc
    18421 cagtctttag gatcgtattt gcgaccattc catttcacac catattgttt cgccagtaat
    18481 gcatacgtct ggcgaacgcg agatccctca attccacgta actgctccac tgaacggcga
    18541 gctggcggtg gttcacgaaa acgtaattca tacattttgc gcaccacctt caaccgtaga
    18601 tcttccgtta aagccagctt tgcctagtag agtaatttat ctgcccgcgc ccctccgggt
    18661 tgtccggagg agtaaacgcg aacgcccgct tcaccgaccc agaccagcag tgttcccacc
    18721 gtggcggcca gatggactgc cgcgtgggaa actcttgttc ccggttcgag cataatgcag
    18781 gcgaccgatc ccactggaat atgcgtgcgg atcccggttt tgtcgatcag cacgaaagcg
    18841 ccgtccagta cgtcgatttg accgtactgg aggaagatca tggaagtgcg atcttttaat
    18901 gggatcgggc tcagcggtac aaacgtcacg cctctgctcc gggtttgatc agcatcagac
    18961 cacaaccgaa cgcccggctt ttgccatacc cctgactgag acgttgcaga aataacccag
    19021 gagcggtgac cgtaagcatc cccgtataat ccacgctact gaactggatc agttgccggg
    19081 agttttcccg ccgcagttgc tgttgtcggt aggcatcaac cgaagtatcc agcagtgtaa
    19141 aaccgcttct ctccccctgc gctgccagcc agtccagcgc agcctgttgt tgatgcaacc
    19201 agacatcact tccttccacc tgccccctca cctgccgttt cgcctccatc agcaagtcgt
    19261 ggcgtttgcc cgctttacaa attgttggat tcgcccgcag gttgaaacaa agggattgtc
    19321 cggtacgcaa ctctggcgca aatggtcggc attcgatggt gaaaatatca ctttccgccg
    19381 ggcgttcctg cgacaggaca aaaaagc
//
LOCUS       DAJVRF010000073        19207 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJVRF010000073 DAJVRF010000000
VERSION     DAJVRF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19207
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.073"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..512
                     /gene="csgD"
                     /locus_tag="OQN13_002370"
     CDS             <1..512
                     /gene="csgD"
                     /locus_tag="OQN13_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCS1615708.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
     gene            517..906
                     /gene="csgE"
                     /locus_tag="OQN13_002371"
     CDS             517..906
                     /gene="csgE"
                     /locus_tag="OQN13_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCS1615709.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            931..1347
                     /gene="csgF"
                     /locus_tag="OQN13_002372"
     CDS             931..1347
                     /gene="csgF"
                     /locus_tag="OQN13_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCS1615710.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1374..2207
                     /gene="csgG"
                     /locus_tag="OQN13_002373"
     CDS             1374..2207
                     /gene="csgG"
                     /locus_tag="OQN13_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HCS1615711.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2272..2763)
                     /locus_tag="OQN13_002374"
     CDS             complement(2272..2763)
                     /locus_tag="OQN13_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCS1615712.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2865..3419)
                     /gene="ycdY"
                     /locus_tag="OQN13_002375"
     CDS             complement(2865..3419)
                     /gene="ycdY"
                     /locus_tag="OQN13_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCS1615713.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3443..4180)
                     /gene="ycdX"
                     /locus_tag="OQN13_002376"
     CDS             complement(3443..4180)
                     /gene="ycdX"
                     /locus_tag="OQN13_002376"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCS1615714.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4235..5173)
                     /gene="ghrA"
                     /locus_tag="OQN13_002377"
     CDS             complement(4235..5173)
                     /gene="ghrA"
                     /locus_tag="OQN13_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCS1615715.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5407..5494
                     /locus_tag="OQN13_002378"
     tRNA            5407..5494
                     /locus_tag="OQN13_002378"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5441..5443,aa:Ser,seq:gga)
     gene            5539..5675
                     /locus_tag="OQN13_002379"
     ncRNA           5539..5675
                     /ncRNA_class="other"
                     /locus_tag="OQN13_002379"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5766..6452)
                     /locus_tag="OQN13_002380"
     CDS             complement(5766..6452)
                     /locus_tag="OQN13_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCS1615716.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHEWADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(7033..7821)
                     /gene="phoH"
                     /locus_tag="OQN13_002381"
     CDS             complement(7033..7821)
                     /gene="phoH"
                     /locus_tag="OQN13_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCS1615717.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(8442..9713)
                     /gene="efeB"
                     /locus_tag="OQN13_002382"
     CDS             complement(8442..9713)
                     /gene="efeB"
                     /locus_tag="OQN13_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCS1615718.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(9719..10846)
                     /gene="efeO"
                     /locus_tag="OQN13_002383"
     CDS             complement(9719..10846)
                     /gene="efeO"
                     /locus_tag="OQN13_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCS1615719.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10904..11734)
                     /locus_tag="OQN13_002384"
     CDS             complement(10904..11734)
                     /locus_tag="OQN13_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCS1615720.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(12400..13908)
                     /gene="putP"
                     /locus_tag="OQN13_002385"
     CDS             complement(12400..13908)
                     /gene="putP"
                     /locus_tag="OQN13_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCS1615721.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            14330..18292
                     /gene="putA"
                     /locus_tag="OQN13_002386"
     CDS             14330..18292
                     /gene="putA"
                     /locus_tag="OQN13_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCS1615722.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(18332..18970)
                     /gene="rutR"
                     /locus_tag="OQN13_002387"
     CDS             complement(18332..18970)
                     /gene="rutR"
                     /locus_tag="OQN13_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCS1615723.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
BASE COUNT         4899 a         5109 c         4665 g         4534 t
ORIGIN      
        1 tggacgatat atcttcaggc tctattattc ttctggatat gatggaagcg gataaaaagc
       61 ttatccatta ttggcaggat actttgagca ggaaaaacaa caatatcaaa atattgttgc
      121 taaatacgcc tgaagattac ccgtaccgcg acattgaaaa ctggcctcat atcaacggcg
      181 ttttttatgc catggaggat caagaacgtg ttgtcaatgg gttgcaaggc gtcctgcgcg
      241 gcgaatgcta ctttacgcaa aagcttgcca gctacttgat tacgcattca ggtaactatc
      301 gttataacag cacggaatca gccctcctta ctcatcggga aaaagagatc ctgaataaac
      361 tgcgtatcgg cgcgtctaat aacgagatcg ctcgttcgtt gttcatcagc gaaaatacgg
      421 ttaaaacgca tctttataat cttttcaaga agatagccgt aaaaaaccgg acacaagcgg
      481 tttcctgggc aaacgataac ctcaggcgat aaagccatga aacgttattt acgctggatt
      541 gtggcggcag aatttctgtt cgccgcaggg aatcttcacg ccgttgaggt agaagtcccg
      601 ggattgctaa ctgaccatac tgtttcatct attggccatg atttttaccg agcctttagt
      661 gataaatggg aaagtgacta tacgggtaac ttaacgatta atgaaaggcc cagtgcacga
      721 tggggaagct ggatcactat aacggtcaat caggacgtta ttttccagac ttttttattt
      781 ccgttgaaaa gagacttcga gaaaactgtc gtctttgcac tgattcaaac tgaagaagca
      841 ctaaatcgtc gccagataaa tcaggcgtta ttaagtacgg gcgatttggc gcatgatgaa
      901 ttctaaataa aaaattgttc ggaggctgca atgcgtgtca aacatgcagt agttctactc
      961 atgcttattt cgccattaag ttgggctgga accatgactt tccagttccg taatccaaac
     1021 tttggtggta acccaaataa tggcgctttt ttattaaata gcgctcaggc ccaaaactct
     1081 tataaagatc cgagctataa cgatgacttt ggtattgaaa caccctcagc gttagataac
     1141 tttactcagg ccatccagtc acaaatttta ggtgggctac tgtcgaatat taataccggt
     1201 aaaccgggcc gcatggtgac caacgattat attgttgata ttgctaaccg cgatggtcaa
     1261 ttgcagttga acgtgacaga tcgtaaaacc ggacaaacct cgaccatcca ggtttcgggt
     1321 ttacaaaata actcaaccga tttttaagcc ccagcttcat aaggaaaata atcatgcagc
     1381 gcttatttct tttggttgcc gtcatgttac tgagcggatg cttaaccgcc ccgcctaaag
     1441 aagccgccag accgacatta atgcctcgag ctcagagcta caaagatttg acccatctgc
     1501 cagcgccgac gggtaagatc tttgtttcgg tatacaacat tcaggacgaa accgggcaat
     1561 ttaaacccta cccggcaagt aacttctcca ctgctgttcc gcaaagcgcc acggcaatgc
     1621 tggtcacggc attgaaagat tctcgctggt ttataccgct ggagcgtcag agtttacaaa
     1681 acctgcttaa cgagcgcaag attattcgtg cggcacaaga aaacggcaca gttgccatta
     1741 ataaccgaat cccgctgcaa tctttaacgg cggcaaatat catggttgaa ggttcgatta
     1801 tcggttatga aagcaacgtt aaatcgggcg gtgttggggc aagatatttt gggatcggtg
     1861 cagacacgca ataccagctc gatcagattg ccgtgaacct gcgtgtcgtc aatgtgagta
     1921 ccggtgagat cctctcctcg gtgaacacca gtaagacgat actttcctat gaagttcagg
     1981 ccggggtttt ccgctttatt gactaccagc gcttgctgga aggggaagtg ggttacacct
     2041 caaacgaacc cgttatgctg tgtctgatgt cggctatcga aacaggggtc attttcctga
     2101 ttaatgatgg tatcgaccgt ggtctgtggg atttgcagaa caaagcagaa cggcagaatg
     2161 acattctggt taaataccgc catatgtcgg ttccaccaga atcctgacag ataaaaatgc
     2221 cggaattaat aatccggcat tttactgttt aagcgaaagc gtgagtgccc ctcacgcttt
     2281 atttttgatt tcctgctcac ggtgagccgt ctttgcacta cgcgccgcca gccacaggcc
     2341 gctgtttttc attgcgtaac caaataccag cccaagcgcc agcgaaggta acaccagttt
     2401 ccagtcaccc tgcccggcaa acgtcgcgca tgcgcctata aacgtccccg gaacaaatga
     2461 aagcagcagc tgtttggcct gaatacacat cagaaacgcc acaataccgg ttatgacata
     2521 accgagaatt tccagatgtg gtgccagtgc actaccgtaa ataatgacca tcgcccacac
     2581 cacgccacta agcagggttg cagcggagat cgccagccct ttcagcccac cttgcgggca
     2641 ggcaaagtag gccgtacagc ccaagaagcc cgcccagctt agtaagccca gggaaacagc
     2701 cacccatccc cagataccgg agagaatgcc tgttgtgatt gcaatagaga gaagtatgtt
     2761 catgccgcgc atgatagcag aaaacgcggc catgaatgag atcgagcaca cattttatgc
     2821 aacaacaaat tatctgttgc agttatgagt gacgatgatc acatttactc ttcagaatct
     2881 tcttccagct cgtcccacat tgcactaatg gcatcacggg ttagcggtgc catggtgcgc
     2941 cagaaaggcg tggttgcatg ggcctccact ttgccgagga acgcaccaca ccagggtaac
     3001 agatactcac tgaacagtgt ttccagtgct tcgctctcat cttccgttga ctgatcttcc
     3061 agccaggaag ccgcaagcag caatgtgccg atgtgatcgg ctggcgtatc cgccagtggc
     3121 atccctcgtt cggaaagaaa agcgcgcact tccgcttccg ttacgtcctc aacccatgcg
     3181 ctacgatatg gcggcactgc acattcatcg ccgataaaca acgcattgta atcggcagag
     3241 acttgcgcca tatcacagct tttctgtaaa cgcgtcagta actcatcctg ctccagtggc
     3301 cagttcgcag ccagtttccc ctcacgaatc agggtaaaca gcggcaccag taaaggatct
     3361 tgcggttggc ggtaatagag cgaacccagc acacgacaga ggatagaaaa ctcgttcatt
     3421 aatatatttc cattactagt aattaaaggt ctgcaaattc cgcaatcggt gccataccgc
     3481 gagattcaag gaagttcagt aagcggcgcg gagaaacatt caaaatgcgc tctggcggaa
     3541 aatctaccgc gtcgaggatt ttaagacact cttcaaattc ccccatggta aacgcggtgt
     3601 gagaatccga gcctaatgcc acccaacctc cagcatcacg taccgctgcg gctaccgcac
     3661 gacagttgtc ttcactgccc ttacgtgagt gtaaaaatga agaattattg atttccagcg
     3721 ccacctgatg tttcgcggct gcttcagcaa cggctttcac atctatttca tatttgggat
     3781 ttccgggatg gcttattata tgcacattgc cgctggcgat agtggcgatc atcgcttgtg
     3841 tattggtcgc tttgtcatgt ggtgcaaaaa ccggctcatg aaaaccggca ataatcagat
     3901 ccagcgagtc aaacatttta ccgctgcaat caatttcacc atcaacattt ttaatgttag
     3961 cttcgatgcc gcgcaggatc cctaccccat caaccactcg cggccagata cgcatgttaa
     4021 tgaagtgcca gtgatgcgga gcatcttcca tatccgggcc atgatcggtg atcgcaaaaa
     4081 gtttaatgcc cttttgtttg gcctgggcaa tgtaatcact caatgtgcta tatgcatgtg
     4141 tgctggcaac ggtatgcata tgaaggtcga cgggatacat aactctctcc tgtattcaat
     4201 tcccgccaag gatagcagga atcctgatgc tttattagta gccgcgtgcg cggtcgactt
     4261 gcccgcagac cctctcccct ttttcgagct gggcaatggt gcgagaaatg tactccacag
     4321 cttcagcggg acgggtaatc gcggcgacat gtggtgttat cgtcacgcgt ggatgttgcc
     4381 agagcggact ttcaggcggt aagggttcac gattaaaaac atccaacatt gcgcctttaa
     4441 ctttgccgct atccagcgcc gcgagcaggt catcttccac aacatgaaca ccacgcgcca
     4501 ggttgaggag atacgcgcca tccggtaatt tttcgagtaa ttgttgatta ataatgccga
     4561 cggtttcagg ggtattcggt aacaaattaa tcaatacccg acattggctc agaaatgcag
     4621 acagttcttc ccgtccggca aagctttgca cgccaggcca cgatttacgg gttcgactcc
     4681 agcaacgcag cggaaagcgc caggtttgca gactctgagc aactttactg cccagtacgc
     4741 ctgcgcccaa aatgccgatg gtaaaatctt cccgatgata ttcaggcagc ggttgccaat
     4801 gcgaactatt ttgctggatg cgataatcgt caaaacgtcg aaaccaatgc agcacctgac
     4861 tgacagcata ttcctgcatt tgctcgccca taccggtatc ttccaggcga aaaagtggaa
     4921 cagaagggtt cagcatttca gggtgtgcct gtagcttgct caaaatagaa tcaacaccgg
     4981 ccccgagtgc gaacaccgct ttaagatcgc gccctgccag catttcaaca ggaggatgcc
     5041 agactaaagc ataatcagca gagtcattat ctccgctttt ccatgctctg actcttgcct
     5101 gaggaatagc tttgcgcagt gcctcaatcc accattgggt atcgaacgtt gggtgataaa
     5161 agatgatatc catactgact cccgaaaagc gttgtgcgaa atttattcgc acttatcgtt
     5221 atgatctaca aaggccacca gcataacaaa tccgtggtcg gtggcaaaaa aagcagattt
     5281 cgcttattaa aaccacacat tgattgaagt ttgaataaac gcgcgatttt ttcaaaaagt
     5341 ttgttgacct caggtcatga tttccctaaa ttagcgcccg ttccagcaag acaggaacga
     5401 caatttggtg aggtgtccga gtggctgaag gagcacgcct ggaaagtgtg tatacggcaa
     5461 cgtatcgggg gttcgaatcc ccccctcacc gccatattta aagaagagct cgtacgaaag
     5521 tacgggcttt tttttcgtat attgcacaca ccggggggat gagaagcccc gaccggggtt
     5581 cgacaactgg cgacagccag ttggacagac cgtgaacgca gtgagcgggc tgcccgcagg
     5641 gcgagcgaag cgagtcaatc cccccctcac cgccatcttt aaagaagagc tcgtacgaag
     5701 gtacgggctt tttttcgtat attgcacaca ccggggggat gagaagcccc gaccgggata
     5761 cttagttaat gcaccggttc actctttgtc gccgcgacta atgggcgaat tttgtcacga
     5821 gtctcccaga ccggaggaat ttcctccaat ttagtgacag aagctcgtac aaaaggcacc
     5881 cgtgcaagag tcataattgt atccaaccaa cggtcacgaa taatgcgatc cgcccattca
     5941 tgactcgggt catccagctc aataacacag actattttat tgttcttctt tgaaaccaga
     6001 acataatcca ttgccatgcc attataaatt gcaaagaaac gctttctttc ttcttcgcta
     6061 aactcaggtt gttctggaaa gttgatcaag cgccccagag agacctggca atggatatga
     6121 caataatgtc caaaccattt ttccagatta tcgaaataca ttttttcaca acgcgtgatt
     6181 aaccactctt ttctttccaa taaaatacct tcggcaacca ttccctgaaa taactcatct
     6241 cgctcttgcc aacgagagtc acgataggtt ttctgcggta tatagctcct tgtcgacgcc
     6301 ttagcggcag gggaagttgc agatgaaggg acgggttgcg atttagcgat gattaccgat
     6361 gaggcaactt ttcggagtgt caattttctg gcaacagcaa aaccggcgat aaatgcaatt
     6421 gtcaaacaag ttacaaaagc cataaagctc ataattaatt ttcacctatc catgttgatt
     6481 atatttcatt accaatgtaa ctcgctgcag cgtgttacat gccaacaatg caacgatcaa
     6541 tatatagtta gtatcatgtt ttgattatta caaccatccc tatttagcca ttgcggcttg
     6601 ccttccacaa aaaaataaca acacacaata ttagcaaaga attaattaag attaacctta
     6661 catttataat attatcccta aagaataaat cagaggaata tattcggtgt taaacagtca
     6721 gtcaaagtat agcccattgt tgattaaacc tcataagcaa aattggcacc agatctcctg
     6781 tacaggttga atggccgaaa aaagataggc agatgctcat ttttttggct tctggaagaa
     6841 aaactctccg cgctggcagt attacactgg tctgatgcta tgacgaaaaa tgcctggtaa
     6901 gagtatctaa accagactcc agttcagctt atcaacacgc tcaaagtcgc ccccctgaac
     6961 gctaacctga agtgaaaatc tgttgaaata aaacaaagcc cggttcgccc gggctctgca
     7021 ccgataacac acttaactgt aggcatgcag cgtacgttgg caaagtgccg aacgtacgca
     7081 gtcctcttta ccgaaccgga cgatcccaac catttcatct tcttcgaaac gttccagcgc
     7141 gtcacttaat ccggagcaca cgccgcgagg caaatcgcat tgcgtgatat caccgttgac
     7201 gataaccgtc acgttctccc cgaggcgggt taaaaacatt ttcatttgcg cggcagtcac
     7261 attctgcgcc tcgtcaagaa tgacgactgc attttcaaag gtacgtccac gcatataggc
     7321 gaacggcgca atttccacct tccctatttc cggtcgcagg cagtactgca taaaggaagc
     7381 ccctaagcgc cggaccagca cgtcgtagac cgggcgaaaa tagggagcaa acttttctgc
     7441 gatatctcca ggtaagaagc caagatcttc atcggcttgc agaactggac gggtgacgat
     7501 aatcctgtcg acatccttat gtatcagggc ctctgccgct tttgctgcgc tgatccaagt
     7561 ttttccgccc ccggcttcgc ccgtggcgaa tatcagctgc ttactctcaa tagccttcag
     7621 atagtgcaat tgcgcttcat ttcgcgcgag gatgggcgaa gtatcgcgac tgtcgcgggc
     7681 cataccaatg gcttctacgc cgcccatctg cacaagcgag gtgaccgatt cttcttcacg
     7741 ctgcttatgg ctgcgcgaat cccgtctcag cacacgtttt gcctcgcgac gagctttgat
     7801 cactgctttt tgtcttccca tagagagcac cttgagttgt ttgtattcat cacacgcgcc
     7861 gttggcagcg cgattatgcg cacgaacatc agagggttgg cttccttgta agccatagtt
     7921 tgcttttgga taaaatgccg aaaaacggct acgcgcaccg tttacggcgt cggtaacaca
     7981 tgaaaagaaa ggatgaggtt gaaaatgcaa agtgacgaga tgactaccgg aggagaaaac
     8041 tccgcgagtg gtggcgcgtt gattatctaa aacatgtcca gtacaggacg ttaccatccg
     8101 cgatctccat agtgactgac tatcactgcc gggaacttcc gctgctactt aataagtaca
     8161 acagatctcg catttattgc aacaatatat ttacttatat ttaactataa aacactattt
     8221 cagtgacatt agtttctact ggaaagatga cagagtgatg acagtgatga aaaaagctgt
     8281 gtgctttcag caggatttgc atcaggcatc agtgcacgat cgccggatgc ggcgtgaacg
     8341 ccttatccag cctaccaaac ccgcactgat tgtaggcatg ataagacgcg caagcgtcgc
     8401 atcaggcatc tgcgcacaaa tgccttatcc gcctaaaaac attaaacccg caataacgcg
     8461 cttccgagat aatcgttcgc gtccttcact cccggcagcg aaaaaaaata accgccgccg
     8521 ataggtttaa cgtattcctc cagcgcttcg ccattgagcc ttttttgtac tgtcaggaag
     8581 cctttttcca gatcgtgttg gtagcagaca aacagcaacc ccatatccag ttgtccggag
     8641 ttggtgaccc ccaacgaata actgtagccg cgacgcagca tcaggctgga ctcactctcc
     8701 gccgtgcggg gattcgccag ccggatatgg ctgtccagcg caatcacctt cccttccggg
     8761 tcgctggcgt aatcaggcac atcatgctca tgctgcattc ccagcggcgc accggtttgc
     8821 ttatcacggc caaaaatcgt ctgctgttct ttcagcggcg ttctgtccca aaattccact
     8881 cgaaactgaa tcaagcgtac tgcctgatag ctgccaccga ttgtccacgc aggctcctgc
     8941 tgatctgccg ttacccacac cactttttgc atcaacttat cattctggct atcgggattg
     9001 gcagtgccgt ctttgaaacc cagcaaatta atcggcgtct ctttgccttt actacgcgac
     9061 acgtgatcgg aaataaaccc ttcccgcttc cagcgcacac tgagcaaatc cggcgtgtgt
     9121 ttgatgatat cgcgcagcgc atggataacc gtgtcctggg tgttggcgca aatctgtagc
     9181 aacacatcac catgacataa cgccgcatcc agcgagtcgt tggggaaacg cgtcatcttc
     9241 tgcagctttt ttggcatctg tggcgcaagg ccaaagcgct catcaaacaa tgagtgaccc
     9301 accgataacg tgatggtgag attatcgggc gcaatgtagc cgccaagaat gccggaatcg
     9361 agtggtggca ggcgcggatt tggcgtttct ggtgctgctc cgccctgagt cagaaaagca
     9421 aaacgctgag tcaacaagcg aaacaaccgc tcaagatcgg ctttatcgct ggcaagcaca
     9481 tcaaacgcca ccagcatcat tgcggcctgt tgtggcgtca ggatccctgc ctgatgctca
     9541 ccataaaacg gctgtttctc attgcgtgca tccggtgaaa gcgtgcccgg tgcactttgc
     9601 gttttttgtg catgagcgac cggacaactt cccgccagcg ccagcgcacc aatccctttc
     9661 agtaaacgtc ggcgtgacgg ttcattcacg ccgttttcat ctttatactg cataacgctt
     9721 aatccagtcc cagcacaccg cgaagttgcg ccagatcttc cgccagcgca gtaatcggtc
     9781 ctttcagtgc attccggtcg gcatcggtca atttgtcgta ggtttcaaaa ccgtctttag
     9841 tacggtattt cgccagaatg gtatcgacct ttttaaagtt ggcatcgact tttgccagca
     9901 gttccgggtt ggctttttgc agttgtggac gcagcaaatc gacaattttc tgcgagcctt
     9961 caacgttagc ctggaaatcc cacagatcgg tgtggctgta gcgatcttct tcaccgctaa
    10021 ttttgctggc tgccacttcc tcaatcagtc cggctgcgcc gccgaccact tttgaaggtg
    10081 ggaaagccag ttcactgatg cgtttttgca aatcgaccac atctgtataa agctgctcag
    10141 cgtactgatc catccctttg gtggtgttgt cgccaaacaa tgctttttcc agacggtgga
    10201 aaccagtgaa ttttgggtcg gcggcttttt gctcgtaatc atcttcacgg gcgtcaatgc
    10261 tgccatccag atcggagaac agttcagcaa tcggttcaat gcgctcatag tgctggcgcg
    10321 tcggcgcata cagtgctttc gctttttcga tatcgcctgc tttaatcgcg tcggtaaagg
    10381 ctttggtgtc ggtcaccagc tgcgtggttt ccgccatgac atacgcttta tatgcagtaa
    10441 ttgcaccacc aagacttaac agcgcatcac tttgcgccgc atccgccgtt gcctcacctt
    10501 tgacgatcaa cttccctttc gggttagtca gcagaccgca ggtcatatcg tattcgccag
    10561 gctgtaaatt cgccgtcatt ttctggctaa aaccaggggc gatattttcc cgctcttcca
    10621 ccaccatcac gcctttgagg atctcccact ccagcgcctt ctggctatgg ttctgaataa
    10681 tgaactgtgt tttcccggcg ttaaccgtaa tggtcatcgg ttcgcactgc ttatccgtca
    10741 cggtcacttt gacctgcggc acatcagcgg cgttagccat aaaagcagaa gaaaacagcg
    10801 cagccacgct caactgcaat gcgttacggc ggaagttaat ggtcatgacg agactatccc
    10861 tttaaagtat gttgtaacta agcaagagtt tgcgtcgtat ttgttacgcg gagcgagacg
    10921 ctgtcgcccc tgcgcgtggt ggcagagcaa atgccaccag cgccgggatg agataaataa
    10981 accagacggc gacttcgctg acgctcggtg cttcctgata gccaaaaatc ccttccatca
    11041 gcgtgccaaa cagcgagtga gttgagagca ctgcgctcat atcgaaggcg atttcctgaa
    11101 agtggttcca caatccggct tcatgaaatg cgcgaatggc accagcagcc agccctgcgg
    11161 cgacgaagag aataaacagg ctggtccatt taaaaaatgc accaagattg aggcgaatac
    11221 cgccccagta gagcaggaag cctagcacca cggcagtagc aagaccgagc attgcaccca
    11281 gcggcggcca gatcccgaca tcttgttgaa atgccgccag caggaaaaag accgactcca
    11341 gcccttccct tgcaacggca aaaaagacca tcatcaccag cgcccagcca tgatgatttc
    11401 cacgctgcaa tgcgctatcg actgcctgct ccagttgtac tttgacgttg cgcgacactt
    11461 tgcgcatcca gaaaaccatc caggtaagga tcaccacggc gatcaccgcc acgatacctt
    11521 caaacagttc ctgttctttt tgcggaaatt cgccggtggt ttcgttaatg aagataccca
    11581 agcccaggca caacgcagcg gcaagcaaca cgccaatcca catcacgcca atccatcggc
    11641 ctcgctgggt acgcttaaga tagctggcaa tcaaactgac aatcagcgcg gcttcaagtc
    11701 cttcgcgcaa cataatgaga aacggaacaa acatgccacc cacccttaaa cgtttttctg
    11761 agtcaatcca tgcaaagaaa agtaaatcgc agtgatagcg attatcatta cggcaaagaa
    11821 aaatacaagc ggaatgttta taaaatgatg ttggaatgta aacaaagaga ggtaatacca
    11881 ggggatataa acgattattc caggtacaaa gatgggattg tttacaacga gccatgtgtt
    11941 cttgccagat gccgcgtaaa cgccttatcc agccgacaaa actgtccaga ctcaatgcat
    12001 cacagcttcc atgtaaaact gataagcggg tgtatcaagc aatttcgtcc atccgcgatt
    12061 atcaaatcta actttttgcc cggaacataa taacagcgcg ggtgttggtg gcgtcaaaat
    12121 cgctgaaacg ttccccgaag gccggggcag cccacatgga tgtgggctga gggcgcgtct
    12181 tacaggacgt tacctcgcgc ccgacccggt agccgaaggg gataagctga aggtaccgcg
    12241 cagcggcgat tttgttcgcc agagcccggg ggtgcagggg gcggcggcga ctggccgccc
    12301 cctgcgcgct ccctgcacca ttcaatttat atggcgaggc acattaaggg agcgcaaccc
    12361 gctgctgaag ccaaacaaaa ccgccgcagg ctaagtccct tagttttcct gcaaccgtga
    12421 cggcggagcc gaatgatagt gcgcatcggc ctcggcaaag cgtttttgca tcgccgctga
    12481 cggcgcttta cccagcaaac taaacactac aatcccgata ctgccgaaga tgaagcccgg
    12541 aataatttcg tacagtccca gccagccgaa ctgcttccag acgataactg tcagcgcacc
    12601 gatgatcatc cccgccagcg caccgttgcg cgtcatgcgt gaccacatca ccgagaacag
    12661 caccaccgga ccaaacgccg cgccaaagcc tgcccacgcg tagctcacta agcccagcac
    12721 gcggttttcc gggtttgccg ccagcgcaat cgccaccagc gccaccacca gcaccatcac
    12781 acgtcctacc cacaccagct ctttctggct ggcctgttta cgcagaaacg ctttgtataa
    12841 atcttcggta atcgcactgg agcacaccag cagctggcaa cttaaggttg acattaccgc
    12901 cgccagaatc gccgacagca gaatcccggc aatccacggg ttaaacagaa tttgcgccag
    12961 ttcgataaac acgcgttcag cgttctggtt taccgcacct gctaccgccg gatgctcgtt
    13021 aaagtaagcg ataccaaaga agccaacagc caccgcccct gccaggcaaa ggatcatcca
    13081 ggtcatactg atacgacgcg catggacaat gctgtggtga gaatctgccg ccataaaacg
    13141 cgccaggatg tgcggctgcc cgaagtaacc cagcccccaa cccatcaatg agataatggc
    13201 aacaaagttc agccctttga gcatatccac gttttcgatg ctcttttgtt tgatcacttc
    13261 cagcgagtca ccaaagccac cgacactgat aatgacgata accggcgtca ggatcagggc
    13321 aaaaatcatc aggctggcct gtacagtgtc agtccagctc accgcgagga aaccgccaat
    13381 aaaggtgtaa aggatcgtcg acgcagctcc cgccaacaga gccgtttcgt aactcatgcc
    13441 aaaggtactt tcaaacagac gcgcgcctgc cacaatgccc gaagcgcaat aaatggtgaa
    13501 gaacagcaaa ataaccagcg cggagataat gcgcaaaatg cggcttttat cttcaaaacg
    13561 cccggtgaaa taatccggca atgttaaggc gttattattg tattcggtat gcacacgcaa
    13621 ccgcccggcc accagcttcc agttgatcca cgcgcctaat gtcaggccaa tggcgatcca
    13681 gctttcggaa atcccggaaa gaaaaaccgc gccaggcaac cccattaaca gccagccgct
    13741 catatccgac gcacccgccg ataatgccgt cacgaatggc ccaagactac gaccgcccag
    13801 aatatagtcg tcaaagtttt tcgttgatcg ccaggcgata aacccaatca atatcatgcc
    13861 aaagatatag acacaaaatg tcaccaacat cggtgtgcta atagccatct aaagtctcca
    13921 aaaaattatt atcggcaatg tcgaaacttg ccgttatatc tgccaccgga acggggtaac
    13981 agagtttatg ttttaccagg gcgaccgtat cctgccggaa gcgctggtta ttcacaatcg
    14041 atttaacaca ccatttacat taaattttag tgctcagcga cactattttt catcaggttg
    14101 cactctctca cattttttgc ggttgcacct ttcaaaaagg ttaactgccg cagagaaaaa
    14161 gtctgagtta ttttttaatc actgtcatat cgatttcttt tattaacatt tcattcattt
    14221 ttaagcttgc tacgcaagtc acatttaaca tggttgcaca aagttgcaac atcatggata
    14281 tttcacgata acgttaagtt gcacctttca gaacaacagg agtaatggca tgggaaccac
    14341 caccatgggg gttaagctgg acgacgcgac gcgtgagcgt attaagtctg ccgcgacacg
    14401 tatcgatcgc acaccacact ggttaattaa gcaggcgatt ttttcttatc tcgaacaact
    14461 ggaaaacagc gatactctgc cggagctacc tgcgctgctt tctggcgcgg ccaatgagag
    14521 cgatgaagca ccgactccgg cagaggaacc acaccagcca ttcctcgact ttgccgagca
    14581 aatattgccc cagtcggttt cccgcgccgc gatcaccgcg gcctatcgcc gcccggaaac
    14641 cgaagcggtt tctatgctgc tggaacaagc ccgcctgccg cagccagttg ctgaacaggc
    14701 gcacaaactg gcgtatcaac tggccgataa actgcgtaat caaaaaaatg ccagtggtcg
    14761 tgcaggaatg gtccaggggt tattgcagga gttttcgctg tcatcgcagg aaggcgtggc
    14821 gctgatgtgt ctggcagaag cgttgttgcg tattcccgac aaagccaccc gcgacgcgtt
    14881 aattcgcgac aaaatcagca acggtaactg gcagtcacac attggtcgta gcccgtcact
    14941 gtttgttaat gccgctacct gggggctgct gtttactggc aaactggttt ccacccataa
    15001 cgaagccagc ctctcccgct cgctgaaccg cattatcggt aaaagcggtg aaccgctgat
    15061 ccgcaaaggt gtggatatgg cgatgcgcct gatgggcgag cagttcgtca cgggtgaaac
    15121 catcgcggaa gcgttagcca atgcccgcaa gctggaagag aaaggtttcc gttactctta
    15181 cgatatgctg ggcgaagctg cactgactgc cgcagacgca caggcgtata tggtttccta
    15241 tcagcaggcg attcacgcca ttggtaaagc gtctaacggt cgtggcatct atgaaggtcc
    15301 gggcatttca atcaaactgt cggcgctgca tccgcgctac agccgcgccc agtatgaccg
    15361 ggtaatggaa gagctttacc cgcgtctgaa atcactcacc ctgctggcgc gtcagtatga
    15421 tattggtatt aacatcgatg ccgaagaggc cgatcgtctg gagatctccc tcgatctgct
    15481 ggaaaatctc tgtttcgagc cggaactggc aggctggaac ggcatcggtt ttgttattca
    15541 ggcttatcaa aaacgctgcc cgttggtgat cgattacctg gttgatctcg ccacccgcag
    15601 ccgtcgccgt ctgatgattc gcctggtgaa aggcgcgtac tgggatagtg aaatcaagcg
    15661 tgcgcagatg gacggccttg aaggctatcc ggtttatacc cgcaaggtgt ataccgacgt
    15721 ttcttatctc gcctgtgcga aaaagctgct ggcggtgccg aatctaatct acccgcagtt
    15781 cgcgacgcac aacgcccata cgctggcagc gatttatcaa ctggcaggtc agaactacta
    15841 tccgggtcag tacgagttcc agtgcctgca tggtatgggc gagccactgt acgagcaggt
    15901 caccgggaaa gttgccgacg gtaaacttaa ccgaccgtgt cgtatttatg ccccggttgg
    15961 cacacatgaa acgctgctgg cgtatctggt gcgtcgcctg ctggaaaacg gtgctaacac
    16021 ctcgtttgtt aaccgtattg ccgacacctc tttgccactg gatgaactgg tcgccgatcc
    16081 ggtcactgct gtagaaaaac tggcgcaaca ggaagggcaa actggattac cgcatccgaa
    16141 aattcccctg ccgcgcgatc tttacggtca cgggcgcgac aactcggcag ggctggatct
    16201 cgctaacgaa caccgtctgg cctcgctctc ctctgccctg ctcaatagtg cactgcaaaa
    16261 atggcaggcc ttgccaatgc tggaacaacc ggtagcggca ggtgagatgt cgcccgttat
    16321 taaccctgcg gaaccgaaag atattgtggg ctttgtgcgt gaagccacgc cacgtgaagt
    16381 agaacaggcg ctggaaagtg cggttaataa cgcgccaatc tggtttgcca cgcctccggc
    16441 tgaacgcgcg gcgattttgc atcgcgctgc cgtgctaatg gaaagccaga tgcagcaact
    16501 gattggtatt ctggtgcgtg aagccggaaa aaccttcagt aacgccattg ccgaagtgcg
    16561 cgaagcggtc gattttctcc actactacgc cggacaggtg cgggatgatt tcgctaacga
    16621 aacccaccgt ccattagggc ctgtggtgtg tatcagtccg tggaacttcc cgctggctat
    16681 tttcaccggg cagatcgctg ccgcactggc ggcaggtaac agcgtgctgg caaaaccggc
    16741 agaacaaacg ccgctgattg ccgcgcaagg gatcgccatt ttgctggaag cgggtgtacc
    16801 ccctggcgtg gtgcaattgc tgccaggtca gggggaaacc gtgggcgcgc aactgacggg
    16861 tgatgatcgc gtgcgcgggg tgatgtttac cggttcaacc gaagtcgcta cgttactgca
    16921 gcgcaatatc gccagccgcc tggacgctca gggtcgccct attccgctca tcgctgaaac
    16981 cggcggcatg aacgcgatga ttgtcgattc ttcagcactg accgaacaag tcgtcgtgga
    17041 tgtgctggcc tcggcgttcg acagtgcggg tcagcgttgt tcggcactgc gcgtgctgtg
    17101 cctgcaagat gagattgccg accacacgtt gaaaatgctg cgcggtgcaa tggccgaatg
    17161 ccggatgggt aatccgggtc gcctgaccac cgatatcggt ccggtgattg atagcgaagc
    17221 gaaagccaat attgagcgcc atattcagac catgcgtagc aaaggccgtc cggtgttcca
    17281 ggcggtgcgg gaaaacagcg aagatgcccg tgaatggcaa agcggcacct ttgtcgcccc
    17341 gacgctgatc gaactggatg actttgccga attgcacaaa gaggtctttg gtccggtgct
    17401 gcatgtggtg cgttacaacc gtaaccagct accagagctg atcgagcaga ttaacgcttc
    17461 cggctatggt ctgacgcttg gtgtccatac gcgtattgat gaaaccatcg cccaggtcac
    17521 tggctcggcc catgttggta acctgtatgt taaccgtaat atggtgggcg cagtggttgg
    17581 tgtgcagccg ttcggcggcg aagggttgtc cggtaccggg ccgaaagcag gcggtccgct
    17641 ctatctctac cgtctgctgg cgaatcgccc ggaaagtgcg ctggcagtga cgctcgcgcg
    17701 tcaggatgca gagtatccgg tcgatgcaca gttgaaagcc gcattgactc agccgctaaa
    17761 tgcactgcgg gaatgggcag caaatcgtcc agaattgcag gcgttatgta cgcaatatgg
    17821 cgagctggcg caggcaggaa cacaacgatt gctgccgggg ccgacgggtg aacgcaacac
    17881 ctggacgctg ctgccgcgtg agcgcgtgtt gtgtattgcc gatgatgagc aggatgcgct
    17941 gactcagctc gccgccgtgc tggcggtggg cagccaggta ctgtggccgg atgacgcgct
    18001 gcatcgtcag ctagtaaagg cattgccatc ggcagtcagc gaacgtattc aactggcgaa
    18061 agcggaaaat ataaccgctc aaccctttga tgcggtgatc ttccacggtg attcggatca
    18121 gcttcgcgca ttgtgtgaag ccgttgctgc gcgggatggc gcaattgttt cggtgcaggg
    18181 ttttgcccgt ggcgaaagca atatccttct ggaacggctg tatatcgaac gttcgctgag
    18241 tgtgaatacc gctgccgctg gcggtaacgc cagtttaatg acgatcggtt aaacaggtag
    18301 ccggaggatg ttacaacctc ctccggcatc tttaacgtgg tcgaatcccc tcaataataa
    18361 tccgctgcac gttttcaacc gtttgattga aaaatatctc atcgcgcaac gtcgcgcctg
    18421 tcaccgcctc cacctgaggg gcgaaatcgg cgtaatgttg agtggaagcc caaatcatga
    18481 aaatcaaatg ctgcggatca atcggcgcga gtttgccgct tttgacccaa ccggcaatca
    18541 gcgcagattt ctcatcaatt aatgccttca aatcgcccgt cagttcatcc attaacagcg
    18601 gcgcgcctgc cagcatctcc atacagaaca ggcgcgaagc ctgcggataa tcgcgggaga
    18661 cttccagctt cagacggatg tactctttta tcgccgccag cggagcgaaa tcttcacgaa
    18721 acgcttttaa cggtgccagc cagatatcga gaatctgccg cagcacggca atatacagcg
    18781 cctcttttga cggaaagtaa tacagcagat tggtttttga aacacccgcc aactctgcga
    18841 tctgctccag ccttgtgccg tgaaaaccga attgtgaaaa agtgtccagt gctgcgctaa
    18901 gaatcgcttt tttcttcgcg cttactgcgc gcgaacgttt acccgttgtt ttcactgcgc
    18961 cttgcgtcat gcgctctccc ctcttgtaga ttggcaacag gatagcaaaa cccccgcccg
    19021 gcctcgacct tctgcactct catcgcgcac agatgcatgt tttatgtgca actgttttga
    19081 ccgtttagtc cactttttat cagatatttt agataacgta atattaacat attaataaca
    19141 ttaacttttt aaaaactggc atccgctttg caaacaagcc aatacgcggc tgaaaaggat
    19201 atgcagg
//
LOCUS       DAJVRF010000074        19084 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJVRF010000074 DAJVRF010000000
VERSION     DAJVRF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.074"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            271..837
                     /gene="yqaB"
                     /locus_tag="OQN13_002388"
     CDS             271..837
                     /gene="yqaB"
                     /locus_tag="OQN13_002388"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCS1615724.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            834..1262
                     /locus_tag="OQN13_002389"
     CDS             834..1262
                     /locus_tag="OQN13_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1615725.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1335..2891
                     /gene="gshA"
                     /locus_tag="OQN13_002390"
     CDS             1335..2891
                     /gene="gshA"
                     /locus_tag="OQN13_002390"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCS1615726.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            3041..3556
                     /gene="luxS"
                     /locus_tag="OQN13_002391"
     CDS             3041..3556
                     /gene="luxS"
                     /locus_tag="OQN13_002391"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCS1615727.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3620..5158)
                     /gene="emrB"
                     /locus_tag="OQN13_002392"
     CDS             complement(3620..5158)
                     /gene="emrB"
                     /locus_tag="OQN13_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCS1615728.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTKRRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5175..6347)
                     /gene="emrA"
                     /locus_tag="OQN13_002393"
     CDS             complement(5175..6347)
                     /gene="emrA"
                     /locus_tag="OQN13_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCS1615729.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6474..7004)
                     /gene="emrR"
                     /locus_tag="OQN13_002394"
     CDS             complement(6474..7004)
                     /gene="emrR"
                     /locus_tag="OQN13_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCS1615730.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(7095..7430)
                     /gene="ygaH"
                     /locus_tag="OQN13_002395"
     CDS             complement(7095..7430)
                     /gene="ygaH"
                     /locus_tag="OQN13_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCS1615731.1"
                     /translation="MSYEVLLPGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7420..8157)
                     /gene="ygaZ"
                     /locus_tag="OQN13_002396"
     CDS             complement(7420..8157)
                     /gene="ygaZ"
                     /locus_tag="OQN13_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCS1615732.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8281..9465)
                     /locus_tag="OQN13_002397"
     CDS             complement(8281..9465)
                     /locus_tag="OQN13_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1615733.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(9756..10748)
                     /gene="proX"
                     /locus_tag="OQN13_002398"
     CDS             complement(9756..10748)
                     /gene="proX"
                     /locus_tag="OQN13_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCS1615734.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGIFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10806..11870)
                     /gene="proW"
                     /locus_tag="OQN13_002399"
     CDS             complement(10806..11870)
                     /gene="proW"
                     /locus_tag="OQN13_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCS1615735.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11863..13065)
                     /gene="proV"
                     /locus_tag="OQN13_002400"
     CDS             complement(11863..13065)
                     /gene="proV"
                     /locus_tag="OQN13_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCS1615736.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(13421..14380)
                     /gene="nrdF"
                     /locus_tag="OQN13_002401"
     CDS             complement(13421..14380)
                     /gene="nrdF"
                     /locus_tag="OQN13_002401"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCS1615737.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(14390..16534)
                     /gene="nrdE"
                     /locus_tag="OQN13_002402"
     CDS             complement(14390..16534)
                     /gene="nrdE"
                     /locus_tag="OQN13_002402"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCS1615738.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTNEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(16507..16917)
                     /gene="nrdI"
                     /locus_tag="OQN13_002403"
                     /pseudo
     CDS             complement(16507..16917)
                     /gene="nrdI"
                     /locus_tag="OQN13_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            complement(16914..17159)
                     /gene="nrdH"
                     /locus_tag="OQN13_002404"
     CDS             complement(16914..17159)
                     /gene="nrdH"
                     /locus_tag="OQN13_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCS1615739.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(17407..17736)
                     /locus_tag="OQN13_002405"
     CDS             complement(17407..17736)
                     /locus_tag="OQN13_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCS1615740.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            17888..18232
                     /locus_tag="OQN13_002406"
     CDS             17888..18232
                     /locus_tag="OQN13_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCS1615741.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(18269..18718)
                     /gene="alaE"
                     /locus_tag="OQN13_002407"
     CDS             complement(18269..18718)
                     /gene="alaE"
                     /locus_tag="OQN13_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCS1615742.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
BASE COUNT         4791 a         4810 c         4929 g         4554 t
ORIGIN      
        1 aaccttcagc aatgaaggta tcgaagagat agcggtatta accgcaggca ccaggaagga
       61 taacgttgcg ccagcaacgg cccgaagggc gaacgtcagt gagtcatcct cccggatgca
      121 ccatcttctc caaaaaattt ccttcaaaaa tatctcattt ctaaataact cctttatcca
      181 tttcctgaat tttcttctcc ttcgccatca gcgacaaaat caagcaatta cgctaaagta
      241 gcgtcatatt tttcgacttg cgagaagacc atgtacgagc gttatgcagg tttaattttt
      301 gatatggatg gcacaatcct ggatacggag cctacgcacc gtaaagcgtg gcgcgaagta
      361 ttagggcact acggtcttca gtacgatatt caggcgatga ttgcgcttaa tggatcgccc
      421 acctggcgta ttgctcaggc aattattgag ctgaatcagg ccgatctcga cccgcatgcg
      481 ttagcgcgtg aaaaaacaga agcagtaaga agtatgctgc tggatagcgt cgaaccgctt
      541 cctcttgttg atgtggtgaa aagttggcat ggtcgtcgcc caatggctgt aggaacgggg
      601 agtgaaagcg ccatcgctga ggcattgctg gcgcacctgg gattacgcca ttattttgac
      661 gccgtcgtcg ctgccgatca cgtcaaacac cataaacccg cgccagacac atttttgttg
      721 tgcgcgcagc gtatgggcgt gcaaccgacg cagtgtgtgg tctttgaaga tgccgatttc
      781 ggtattcagg cggcccgtgc cgcaggtatg gacgctgtgg acgttcgctt gctgtgagtg
      841 aagcgttatc gcttttctcg ttgtttgcca gtagttttct cagcgctaca ctattacccg
      901 gcaactccga agtcgtgctg gtggcaatgt tgctttccgg gatcagtcat ccctgggttt
      961 tagttttaac agcaacaatg ggtaatagcc ttggagggtt aactaacgtt atccttgggc
     1021 gtttctttcc attgcgtaaa acatcgcgct ggcaagagaa agctatcggc tggctgaaac
     1081 gctatggtgc agtcacgcta ttattaagct ggatgcccgt ggttggcgat ttactgtgtc
     1141 tgttagcggg atggatgcgc atctcgtggg gaccggtaat cttttttttg tgccttggta
     1201 aagcgttacg ctatgttgca gttgcagcag cgaccgttca gggcatgatg tggtggcact
     1261 aattgtaggc ctgcacatat ggtcaccatt acagttatgc taattaaaac gattttgaca
     1321 ggcgggaggt caatttgatc ccggacgtat cacaggcgct ggcctggctg gaaaaacatc
     1381 ctcaggcgtt aaaggggata cagcgtgggc tggagcgcga aactttgcgt gttaatgctg
     1441 atggcacact ggcaacaaca ggtcatcctg aagcattagg ttccgcactg acgcacaaat
     1501 ggattactac cgattttgcg gaagcattgc tggaattcat tacaccagtg gatggtgata
     1561 ttgaacatat gctgaccttt atgcgcgatc tgcatcgtta tacggcgcgc aatatgggcg
     1621 atgagcggat gtggccgtta agtatgccat gctacatcgc agaaggtcag gacatcgaac
     1681 tggcacagta cggcacttct aacaccggac gctttaaaac gctgtatcgt gaagggctga
     1741 aaaatcgcta cggcgcgctg atgcaaacca tttccggcgt gcactacaat ttctctttgc
     1801 caatggcatt ctggcaagcg aagtgcggtg atatctcggg cgctgatgcc aaagagaaaa
     1861 tttctgcggg ctatttccgc gttatccgca attactatcg tttcggttgg gtcattcctt
     1921 atctgtttgg tgcatctccg gcgatttgtt cttctttcct gcaaggaaaa ccaacgtcgc
     1981 tgccgtttga gaaaaccgag tgcggtatgt attacctgcc gtatgcgacc tctcttcgtt
     2041 tgagcgatct cggctatacc aataaatcgc aaagcaatct tggtattacc ttcaacgatc
     2101 tttacgagta cgtagcgggc cttaaacagg caatcaaaac gccatcggaa gagtacgcga
     2161 agattggtat tgagaaagac ggtaagaggc tgcaaatcaa tagcaacgtg ctgcagattg
     2221 aaaacgaact gtatgcgccg attcgtccaa aacgcgttac ccgcagcggc gagtcacctt
     2281 ctgatgcgct gttacgtggc ggcattgaat atattgaagt gcgttcgctg gacatcaacc
     2341 cgttctcgcc gattggtgta gatgaacagc aggtgcgatt cctcgacctg tttatggtct
     2401 ggtgtgcgct ggctgatgca ccggaaatga gcagtagcga acttgcctgt acgcgcgtta
     2461 actggaaccg ggtgatcctt gaaggtcgca aaccgggcct gacgctgggt atcggctgcg
     2521 aaaccgcgca gttcccgtta ctgcaggtgg gtaaagatct gttccgcgat ctgaaacgcg
     2581 tcgcgcaaac gctggatagc attaacggcg gcgacgcgta tcagaaagtg tgtgatgaac
     2641 tggttgcctg cttcgataat cccgatctga ctttctctgc ccgtatctta aggtctatga
     2701 ttgatactgg tattggcgga acaggcaaag cgtttgcaga agcctaccgt aatctgctgc
     2761 gtgaagagcc gctggaaatt ctgcgcgaag aggattttgt agccgagcgc gaggcatctg
     2821 aacgccgtca gcaggaaatg gaaaccgctg ataccgaacc gtttgcggtg tggctggaaa
     2881 aacacacctg acagaaaaga aaaaggccac tcgtgagtgg ccaaaatttc atctctgaat
     2941 tcagggatga tgataacaaa tgcgcgtctt tcatatactc agactcgcct gggaagaaag
     3001 agttcagaaa atttttaaaa aaattaccgg aggtggctaa atgccgttgt tagatagctt
     3061 cacagtcgat catacccgga tggaagcgcc tgcagttcgg gtggcgaaaa caatgaacac
     3121 cccgcatggc gacgcaatca ccgtgttcga tctgcgcttc tgcgtgccga acaaagaagt
     3181 gatgccagaa agagggatcc ataccctgga gcacctgttt gctggtttta tgcgtaacca
     3241 tcttaacggt aatggtgtag agattatcga tatctcgcca atgggctgcc gcaccggttt
     3301 ttatatgagt ctgattggta cgccagatga gcagcgtgtt gctgatgcct ggaaagcggc
     3361 aatggaagac gtgctgaaag tgcaggatca gaatcagatc ccggaactga acgtctacca
     3421 gtgtggcact taccagatgc actcgttgca ggaagcgcag gatattgcgc gtagcattct
     3481 ggaacgtgac gtacgcatca acagcaacga agaactggca ctgccgaaag agaagttgca
     3541 ggaactgcac atctagtcag taaactatct tcacaattaa ttgaaaaaag ccagttcaaa
     3601 tgaactggct tagttgtact tagtgcgcac cgccgccacc gccacctgcg ccaaatggcg
     3661 gtttagcaaa ccacaccagc cccagcagta cgaggaatat cccggctgac atccagaaga
     3721 tctcattggc ggaaataatc agcccctgat tggtgatctg ctgggcaatc cagcctgatg
     3781 cctgctgttg cgtcatccca agcccttcca gttgactgta catcgcctgg gcattcgggt
     3841 tgaacgggtt taccgactca gtcaactgcg catggtgcat cgactcgcgg ttggtccaca
     3901 tggtcgtggt tatcgacgtg ccgatagacc ccgccagcgt tcgcgtaaag ttagagaggc
     3961 tcgatgccgc cgccagtcgt tccggtggca aaccagacag cgtaatggtg gtcagcggca
     4021 taaagaagca ggccaccgca aacccctgga taaactgcgg ccaggccgac gcgccaaaat
     4081 ccatacctgg ttcaaaggta taggcacgcc agtagaagca gacggcatac ataataaagc
     4141 tgaaggttac cagccgccgc atatccagtt tatgcgcgaa gcggccgata atcggcgaca
     4201 ggatcaccgg aataatccct accggcgcag aggccaaacc tgcccaggtc gccgtgtaac
     4261 cgtagacctc ctgcaacaac tgcggcagca gaacaatagc gccgaagtag agcatatacg
     4321 cgaggctgat acacaagcag ccgatggtga agttgcgcga cttaaacaac gacagatcga
     4381 ctatcgggtt atcgtcggtc agctcccaga caatcaggaa gcagatagcc accaccgcca
     4441 ccacggtaag gatgataatt tcctgtgatg aaaaccagtc cagctcttta ccgcggtcga
     4501 gcataatctg caggctgccg ataccaataa ccagcagtgc cagccccacg gcatcaatcc
     4561 gccgccgttt ggtgcgggtt tcacgtccgc gcagagtttg cagtgtcatc aacaccaccg
     4621 ccacgccaat cggcacgttg atgaagaata tccagcccca gtggtaatta tcgctgatat
     4681 aaccgccgag gatcgggccg caaattggcg cgacaatcac cgtcatcgac cacaacgcca
     4741 gcgcgatcga gcgtttggct ggcgggtagt tattcagcaa tagactttgc gaaagcggga
     4801 tcaacggccc ggcgacaatc ccctgaatca cgcggaagaa gatcagcata ttcaggctgc
     4861 tggagacacc acacgcccac gacgcaatag caaaggcgat ggtggaccaa aggaacagtt
     4921 tcacttcccc gacgcgcttt gccagccagc cggtaagcgg gatcgagatg gcattcgcca
     4981 ccccgaaaga agtgattacc cacgttccct ggctgagcga tgagcccaga ttcccggcga
     5041 tagtggggat cgccacgtta gcaatggtgg agtccagcac ctgcatgaat gtcgccagtg
     5101 acagcgcaat cgtcataatg accagttgcg cgccttccag cggtttttgc tgttgcatca
     5161 cacgcacctc tggattagcc agcgttagct tttacgatat cgtcgatcag tttattgaca
     5221 ggtgccaggc tgatttcacg cgcggtactt accgcgaccg gtgttgaacg tactttattt
     5281 gccagtacct gaccgtcacg gttagtggta ttgacgctca ccagtgtgga caaaccaatg
     5341 cgcagcgggt attgctccag ctgtttctgg tccagttcga tacgcacagg tagacgctga
     5401 acgactttga tccagttacc ggtcgcattt tgcgccggaa gcagtgagaa tgcgctacct
     5461 gtgcccatat ccagaccaac cactttaccg gtgtatttca catcatcgcc gtaaatatcc
     5521 gtggtgatag tgaccggctg accgatacgc atattggcaa tctgcgtctc tttaaagttg
     5581 gcatccaccc acatattggt ggctggaacg accgccatca gcggcgtcgt tgggctaatt
     5641 tgcgccccag gctgtaccgc acggcgggag acataaccgg tcatcggact gacaatacgg
     5701 gtacgctcca gcgccagcca ggcgttacgt acttcggtgg cagcctgttg cacggcaggc
     5761 tgatcttcca gtttagtccc cagaatcatc gcctgattgg cattgtattg ttgaatagcg
     5821 acgtccagtt gcgcctgggc actggtgacg gcgtcgcggg cgtgttgcag ctcttcgcga
     5881 ccaatcaggt tggcattgcc cagcggcaca cggcggttgt agtcgctttg tgcttttgcg
     5941 agggcgattt tctgcacctc aatattcgcc tgcaactgtt tgctgttaat catcagctgg
     6001 tgggtttggc gaacgctgga agccagtgca gttttggctt tttcaaacgc ctggcgagca
     6061 tctgtcgggt cgagagtgac cagcacgtcg ccttctttta caaaatcggt gttatcggcc
     6121 cagactttcg tcacgctgcc agacacctga gacataattt gaatctgatt ccctgccacg
     6181 tatgcgtcat cggtttcttc gaaatgacgc agtaccaaaa accaataaat ccctatcgct
     6241 acggcaataa ttataaagag caaggtgaga aggaggagca gacgcttacg tttgccgctc
     6301 ttctttaccg gttgctgcgg ggtttgagtc tccgcatttg cgctcatatt gttctccacg
     6361 atcttcttaa ctcatcggct gagccgacct gcttgccaaa aaggccagca tgttgcgatg
     6421 ctggccggtc attttttctt ttataaatct ggatttttga gcgagatgac gcgttagctc
     6481 atcgcttcga gaaccacacc gtcttgttcc atctggtcga gacgggagag caatttgcgg
     6541 gtgatttgct cgagctgatc tttttctgtt gtgctgagcg cggaccagag ttgatgcagg
     6601 cagttatgct gcggtggtaa aacctcgcgc aaaaactcgt gacctttttc cgttaattgc
     6661 agatgcaggc agcggcgatc gttatcgctt tcacgacgtt cgatccaacc gcgtttttcc
     6721 agttcatcgg caatacgcgt cgcgttggta cgggatgatc caagagcaca acttaattca
     6781 gaaggctgaa tactgtggtt ttcctgagac tccagcgtaa tcaacgccat aaacaacgtc
     6841 tcgttaatcc cctgagcctt cagcatttta ttgcggttct ccagcagctt gctttgcatg
     6901 tgcatgcaaa gacgagtcag aaggatctcc tgataaggaa aatcttcgtg gcggctggcg
     6961 cgaaatttta gcatttgttc aatgggcgta aacgaactat ccatttgggt atgacctcat
     7021 taatttcagc cgatatagta acgacagtga caaataaagt aaatgtattg ttttagaaaa
     7081 atgattcttg tgggttatat aatcgccatc actttccagg cgagcccata ggccagcgca
     7141 ctaagcagtg ttgggatgat aatgctgcgt gttttataga aactggcacc cagtaccgcg
     7201 aagccgacca gcgtgggcac gaaacggcgt gtatcgtgca tcacttctgg tgcggtagag
     7261 acaaccagca gagcgcatat cgaggcgatg ccaatggtgt cgagcaaaat acctaccgcg
     7321 ccacgtttgg ttgggcgggc attacccaca cgcaggcgca gcggcaaata gcggaagcaa
     7381 taattcgccg cgccaaccag taacccaggc agcagaacct catagctcat cgggcgctcc
     7441 ttgccagaat gcttggatta acgcagtgag gcagccacag acaatgcctg ccagaatggc
     7501 gacgggaata gaaaatagcg ttacgcctgc aagggcacca actaacgctg cggtaacgca
     7561 aagagattgt ttgcgctgga aagaggcgag caggaaactc ataaagagtg ccggaagcat
     7621 aaaacctaat gctgcttcaa cggcgggata accttgcagc aagccgctgc cagagaatgc
     7681 ccctattacc gtaccaaata cccacgatga ccatgaactg aaggcaatgc cgatcatcca
     7741 gttctcgctc cagcggcgat tattgcgtac cagttttgct gttgcggcgg caaaaacctc
     7801 atccgtcagg ccaaacgccc acagggcggt tttcgatttt tgcagacgct gaataatacg
     7861 gctacgcagt gacgggccat acaacacatg gcgaacatcc attgccatga cggtcagtgc
     7921 agcaacccac aaactactcc cggctgccag catcgcggta atgacgaact ggctcgcgcc
     7981 tgcataaatg atgcaggaga aaaaaacgct ttcgagagga gagaatccca gacgggtcgc
     8041 attcagaccg aacgcaaagg ccaccggaat ataactaata acaatcggta aactgtcttt
     8101 gcatccttcc atgaaggtcg ccgaaccagg agcaggctgt ggagtagggc tttccataga
     8161 gtgtacgctt aacgattgtt aatattataa atagactgaa tgaatatctt aaccttatca
     8221 gactgatggg cttcttaaca cccttataag tgtaaagcca cgaaaacggt tgctgattgc
     8281 ttaatttgcg gcttcctgac gatgaaaacc tcgccaccag accagtaagt taactagggc
     8341 aatcgtcgcg ccagccagac aaacgccagc ccaaccgcca tgttgccagg ctgaggctga
     8401 aattagcgaa ccggcggcac cgccaataaa gtagctggtc atgtaacctg cggtcaggcg
     8461 attacgcgca tcagggtgta ttcgataaat taccgtctgg ttagtgatat gcacgccctg
     8521 tacggtgaga tccagcacca ggatgccgat aatcagcgcc agtacggaag tgtgaccaaa
     8581 ccagatcgcc agccatgaaa gtaatagcag cagcagaccg aaagttgtgg tgtggtgcga
     8641 tttaccctta tcggcaaaac cgcccgccgg acgagcgccc aacgctccgg cagctcccgc
     8701 aagtccaaac agaccaatga caccatcgct gtagttaaaa ggtggagcgg caagcaaaaa
     8761 ggccattgag gtccagagaa tgctgaagtt ggcaaaggtc aggcagccca gcaatgcgcg
     8821 ggtgcggagg atcttatcgc tgataaacat gctgaaaacg gaacctaaca actgtgggta
     8881 gttgaggtgg gtttctgatt tcatttgtgg cagaccacgc cataatgcta gcgccatcag
     8941 tgccattaac accgaggcaa cccaaaagac ggtgcgccag ccgccgaggt ttgccagtaa
     9001 cccggcaacg gttcgtgcca gcaagatccc caacagcaga ccgctcataa tggtgccaac
     9061 cactttgccg cgtttatccg gtgaagccag cgtcgctgcc agcggaacca gaatttgtgc
     9121 cacgactgag aataaaccgg ttaatgccgt accgaggatc atcatcgcca gcgactgact
     9181 gctggcagtg attaacatgc cgcctgccgc cagtaaggtc atcgagacaa tcaggcggcg
     9241 gcgttcaaac atatcaccga ggggaacaag aaacagcaga cctgctgcat agcccaactg
     9301 cgcggcggta acaataaagc ctgccgaact ggcggaaagg gaaaagttac gcgcgatggt
     9361 gtcgagcaat ggctgggcgt aatagttact ggctaccgcc tgaccggtgg cgatagacat
     9421 cagcacgatc agcgccgggc taagctcatg attaggttta gtcattattt ttgttcatca
     9481 ggtgatgatt aagctggaaa tcaataatag cggatgttga tgaatggggg cgaaagatag
     9541 cttgttgaaa tgtgcgatgt ggtttgtagg catgataaga cgcgccagcg tcgcatcagg
     9601 cattgtgcac agaacggcgg atgcggcgaa aacgccttat ccgccatacg gaatgagtgt
     9661 attttgtagg catgataaga cgcgccagcg tcgcatcagg cattgtgcac agaatggcgg
     9721 atgaggcaaa aacgacttat ccgcccgaat aaaaattact tctgcgctgc cagcgcctca
     9781 ttcacccagc catcgaactg ctgctggtgg gctttgatcc aaccatcaac atggccctga
     9841 atatcgcctt ctgaggcttt gccgtcatgc ataatggcgt tctgggcgtt aatatctgcc
     9901 actggcaact gcataatggc aaaaagtttc gctgctgccg ggtttttctc ggcccaggct
     9961 ttgttggcaa cgatatgcat ggtgctgacc gggaagccat aattcgcacc attcggcagt
    10021 ttggtatcgg cgtttttatc gcccggcagt gcggagaacg gcacctgcaa ccagaccaca
    10081 tctttccctg gcttcagctc attactcacc cagtacggcg tccaggtgta ataaaacacc
    10141 ggtttgccct ctttgtagcg actgatggtg tcggccatca tcgctgcgta gttcccctga
    10201 ttatgcgtca cggtgttggt cagttcatac gcggcaagct ggtggttgat cgcaccttcg
    10261 cagccccagc ctgggttaca gccggttaaa tccgctttac cgtcgccgtt ggtatcgaac
    10321 agtttggcga tcttcggatc tttcagttgt gcgatgttgg tgattttgta ctggtcggcg
    10381 gttttcttat cgatcaggta accctgtgcc gcgccgttaa cgaatatccc ttcacaataa
    10441 aatttcttat cgccaccggc agcttcgtac atgttgtcat gcagcggcgt ccagttcacg
    10501 gcggtgaagg ttgcatcgcc ggaagcaagt gaggtgtagc caacgttgta atccacttcg
    10561 ctgggtttat tgacggtata acctaatttc tccagtgcac gactgaccag cagcgtctgg
    10621 aaggtttctt cagtgatggt gctctgaact ggattaacag taatgccttt gcccggcaga
    10681 tcggctgcaa aagtttgtgt agagataagc gtggcaaacg ctgtcgcaaa aagtacgcta
    10741 tgtcgcattg ttattccttt ttttggcagg gtgataatgc ccggcgttac cgggcaagtg
    10801 cagagttact taatgaatgg gcgggtcagc agaccgacag ggccagtggt gtaccagcga
    10861 cggttgccgc gactgcgtga gtcgcgccca acggcctgcg tcaggcggtc gaggataatg
    10921 gcgaggatca caatcccgac gccgccaacg gtggcaaggc ccatatccag acgaccgata
    10981 ccgcgaagta ccatctgacc cagcccgccg acggcaatca tcgaggcgat gaccaccata
    11041 gaaagagcca gcatcagcgt ctggttaacg cccgccataa tggtcggcat cgccagcggt
    11101 aactgaactt tgaacagcat ctggcgcggg ctggcaccga atgagcgcga ggcttcaatc
    11161 agatccgccg gaacctggtt aatccccaga atcgtcagac ggataatcgg cggcagagca
    11221 aagatgatcg tcaccactac gcccggcacg ttaccgatac caaacagcat gacgattggt
    11281 accagataaa caaacgctgg cgtggtctgc atggcatcaa gcagtggacg aataattttc
    11341 gccgctcgcg gacttctcgc cagccatatc cccaacggca aaccgatgac gatacagaac
    11401 agcagggcgg ttaacaccag cgccagagtc accattgcct gcgaccaggc accgattgcg
    11461 ccgatggcaa tcagcgaaac cagcgtcgcc acgcccattc cgaccccgga aatctgccag
    11521 gcgatgagag cgaaaacgat aatcgccact ggcgctggca tacccagcag caattgctgg
    11581 aaactgttga ggatataatc aaccggaacg cgcacgccct ggaagacggg acggaaatgg
    11641 gtaacaaccc agtcgatccc ttcagtgacc caactgtcga gcgggatcag cgttttatgg
    11701 aacggatcga gaatattaaa atgctcgacg tttggcgcag gcgtactggt cagccagtca
    11761 gcaccaccgc cgtcagtcgg tgcagtcgtc ggtgtacccc aggcgtctgc ggattgcgcg
    11821 gcactgtccg ccgctggcgt ggtatcccac ggattatttt gatcagccat tatttacccc
    11881 ctcacgatct aaagcgcgca gcagcattcc tttcgaaatg atgccgacat actgttggtc
    11941 ctcgtcgacc acgggcaccg cacagggtgc ctgtccgaca tgagagagca actcgctaag
    12001 aggcgtttgt gcatcgactg ctaacggcgc atcaatcagc gccgcatcaa gaccttgctg
    12061 ctgcgttaac gcggttttaa gcgaatcgat ggagactgcg ccgacaaact tattaccgcg
    12121 ttcgataacg tagccatatt cgcgatcttc atcctgcaat aatttcagtg ccgaacgtgg
    12181 gccgaagcca ggggttttac gaattaagcc attcggtgtc cggcgggcaa tatctttcgc
    12241 actgaatacc tgactaatat caacgccacg gaagaaggta cggacataat cattcgccgg
    12301 attattgaga atttcatccg gtgtgccgac ctgtaccact tcaccatttt gcataatggc
    12361 aattcggtcg ccaatacgca tggcttcatc gagatcgtgg gaaataaaga caatggtgcg
    12421 ctgatgtttc gcctgtaatt ttaccagctc atcctgcatc tcggtgcgaa ttaatggatc
    12481 gagcgccgag aaggcttcgt ccattaataa aatatccgga ttaatcgcta acgcgcgggc
    12541 taatcccaca cgttgacgca tcccgccaga gagttcatcc gggtagctgt gggcataatt
    12601 ttccagcccg acctgacgca gtgcatcaag ggctttttcc cggcgttctt cggcattaat
    12661 tccggccaat tccataccga acgcagtatt gtccagcacg gtcatatgcg gcattaaggc
    12721 aaaggactgg aagaccatcg caatcttttt tctgcgcacc tcacggagtt tggcatcaga
    12781 tattttggca atatccacac catcaatcag cacttgcccg cgggtgggtt caatcaggcg
    12841 attgagaagg cgtaccattg tggatttacc cgagccggat aatcccatga tgacaaatat
    12901 ctcgccttct tcaatggcca gactggcgtc ttttacgcca agcgatagcc cagttttttc
    12961 cagaatttgt tcttttgaaa gtccttgttc gatatatttg aatgctcgct gtggatgctc
    13021 accaaatatt ttataaaggt ttttaatttc taatttaatt gccatgcaat agaaagattc
    13081 ctgttatttg tttatgtcga tatattaccc aatggaaata gttacttttt tctaccctaa
    13141 catactgaga atctgaggca acccctgatg gcaaatgtgg catgaatatt gatgcgagca
    13201 aatcgccgga atttcccgtg atataagggc tgagagcaaa tcgagaaaag ttgatatttt
    13261 ttgtcaaaga tagcgaaaaa acgcctgaat attgtggttt caggcgaata atgcaacaga
    13321 gtatcagtgt agatcaccac aaaatatttg cgtgataaaa agctatttgg cgggaattat
    13381 ttccctgtcg cgggtagtga tatttttgaa aataacaccc tcagaaattc cagtcttcat
    13441 cttctgtttc aaccgctttc cccatcacat aagaggagcc tgaaccggaa aaaaaatcgt
    13501 gattttcatc ggcattcggc gaaagcgcgg cgaggattgc cggattcact tccgccattt
    13561 ctgcgggaaa taaaggttcg tagcccagat tcatcaaagc cttattggcg ttgtaacaga
    13621 gaaacgcttt cacatcgtca gcccacgggg tttcggcgta cagctcatcg gtgtattgca
    13681 gctcgttgtc gtagagttcc agcaacaaat cgaaggcgaa actcttcaac tcttcacgtt
    13741 gtcccagaga tatcttttcc atgtttttct gatatttata gcctatgtag taaccgtgga
    13801 ctgcttcatc acggataatc agacggatca ggtctgcggt attggtcagc tttccgcggc
    13861 tggaaaaata catcggcagc cagaaaccgg aatagaacaa aaaagattca agaaacacac
    13921 tggcgatttt ctttttcagc ggatcatcac cgcgataatg ttgctgaata atctgagctt
    13981 ttcgctgcaa cggtgcgttt tcttcactcc aggcgtaggc ggcatcgaca tctttggtct
    14041 ggcacagcgt cgagaaaatc gaactgtaag agcgggcatg aaccgcttcc ataaagctga
    14101 tattcgataa taccgcttct tcatgaggcg tgagtgcatc gggcatcagc gaaggcgcgc
    14161 cgataacatt ttgcagcgtg tcgagcagcg tcaggccagt aaaaacgcgc atcgtcagtt
    14221 gttgttctac gacagttaat gtctgccagg caggaatatc gttcgacagc ggcacctttt
    14281 ctggtagcca gaaattgctg gtcaggcgat tccacacctc cagatcttta tcgtcagata
    14341 tcttgttcca gttgatggcg ctgatacgtg agagtttcat agatattcct taaagtgcac
    14401 aggagacgca gccttcaatt tcagtgcctt ccagcgccat ctgacgcagg cgaatgtaat
    14461 agagcgtttt gatacctttg cgccaggcgt aaatctgcgc tttgttgata tcgcgagtgg
    14521 tggcggtatc ggggaaaaac agcgtcagcg acaaaccctg atcgacatgg cgagtcgctt
    14581 ccgcgtaggt gtcgatgatc ttttctgcgc cgatttcgta agcatcctga tacagcgcca
    14641 gattttcgtt agtcataaac ggggcagggt agtaaacgcg tcctgttttg ccctctttgc
    14701 gtatctctac tttcgccaca atcggatgaa tactcgacgt agcatggttg atataagaga
    14761 tagaaccggt tggcggcacc gcctgaagat tctggttgta aatgccgtag cgcatcacgt
    14821 cgtcgcgcag ttgcgcccac atctcacggg taggtaacgt aataccgctg cgggcaaaca
    14881 gttcgccaac tttcgccgtt ttcggctgcc agttaccttg cagatattgg ctaaaatatt
    14941 caccactggc ataacgtgac tgtttgaacc cggcgaaggt ttcaccgcgt tcgcgcgcca
    15001 gcaacatcga ggtacgcagt gcgtgccagg tgatggcata gaaatagaga ttggtgaaat
    15061 ccagtgcttc cggcgaacca taagcgatgc cttctcgcgc cagatagccg tgtaaattca
    15121 tctgccccag tccgatggcg tgtgaggcgg catttccggc ttcgatggac ggcacgctgc
    15181 ggatatgact catatctgac accgccgtta aaccgcgcac ggcagtctct accgtgcggg
    15241 caaaatcggg ggaatccatg gtgtgcgcaa tattcaacga acctaaattg caggagatat
    15301 catggcctgt gcgggcatag tcgagattct cgtcatactc tgaggcacag ttgacctgca
    15361 aaatctctga gcagagatta ctcatattta tgcgcccggc gacagggtta gcacggttta
    15421 ccgtgtcttc atacatgatg tagggatagc cggactcaaa ctggatttct gccagccgct
    15481 ggaagaaatc acgggcgttg aggtattttt tgcggatgcg ttcatcggca accagttcgt
    15541 catagtgttc gctgatggcg atatcggcaa acggcttgcc ataaactcgc tcaacgtcat
    15601 agggcgaaaa cagcgccatc tgcgcattct cttttgccag atggaaagtg atatccggga
    15661 tcaccacgcc cagcgacagt gttttaatgc ggattttttc gtcggcattt tcccgttttg
    15721 tatcgagaaa acgcagaata tcgggatgat gagcatgtaa atagactgca cctgccccct
    15781 gacgcgcgcc gagttggttg gcataggaaa atgcgtcttc cagcattttc atcaccggaa
    15841 ttacgccaga agattgattt tcaatacgtt taattggcgc gcccgcttct cgcagattcg
    15901 acagcaaaaa tgccacgccg ccgccgcgtt tcgacagttg cagtgcggaa tttaccgccc
    15961 ggccaatcga ctccatattg tcttcaatac gcagcagaaa acaggaaacc agttcgccgc
    16021 gctgctgctt accgcagttg aggaatgtcg gcgtggctgg ctgaaagcgt cctgacagca
    16081 tttcattggt cagttgcaac gccagcgtcg catcgccttg tgccagcgtc agcgccacca
    16141 tcgtgactcg atcggcaaaa tcttccagat aacgtttacc gtcgaatgtc ttcaacgtat
    16201 agctggtgta aaacttccat gccccgagga atgtctggaa acgaaaaccg ctggtgtgtg
    16261 cgtgggcaaa cagcgtaatg acaaagtcgc gagagtagcg attaagaacg ctttcatcat
    16321 agtaaccttc gttgaccagc cagttcaggc gctgctgctg gctactgaag gtcacactgt
    16381 tcggacgcac atgcgtcgcc ataaaggcgt caacggcctg gcggtcttta tcgaactgaa
    16441 tgcgacctgc gctatcgtag aggttaagca tcgcattcag cgcgtggtaa tccatcgttt
    16501 cctgcgtcag gcattctgcg gttgtcgttg ccaaaattcg gttactcctt tacgaacgtt
    16561 ttcgatatcg ctttgcgtac ccatgagttc aaaacggtac agccacggca cgccgcattt
    16621 ccgggcaatc acatctccgg cgcggccata cgcctcacca aagttgcgat taccagaagc
    16681 aataacgccg cgaagcaacg cccggttgtg ctcgtcgttt aaaaagcgaa ttacctatcg
    16741 tggcaccgcg ccagccgtac cgccgccgcc gtaagagggc acgatcagga tgtaaggctc
    16801 gtctacctga atccgttccc gctcattgag cgggatgcgc accgcgggca gacctaaacg
    16861 ttcgataaaa cgctgcgtgt tttcggagct gctggagaag tagacgagct ggctcatgca
    16921 ctggccgcgt gtggcgctgg atgcagacgg ttaatcatgt ccggacggaa accagaccag
    16981 ctaagatcgc cagcaatcac taccggcaac tgacgaaagc cctgagcacg caacgcttcc
    17041 gctgcttcag gaacgcgatc gacattaatc atttcaaaat caaagccccg gttttccatc
    17101 gcccgtttgg tggcgtggca ctgaacgcaa tcgttacgag tgtaaatagt tatgcgcatg
    17161 attcgtattt ccgtttaaaa tgaagatacg gcgcgatgat acgcgtcggg ttgtctctct
    17221 gttgatacag agatactaga tgtagttgaa aaaagattca accacacaat atatagcaaa
    17281 tagtgatcga aattacccta gatatgagcg tgacggggga gggggatttt tgtgattcac
    17341 caggcaaaaa gaaaccccga agacaggctt cggggttaaa gacgcgtatt tattatcatt
    17401 tttgcactac gatttgcgca tgcttaacag tgcgccgata aaaataccta ccgcagctgc
    17461 tgtacccacg ctacaccagg gtctttcacg aacaaaagaa tccgcgcagc caacggcatc
    17521 gcgtgcggct tgctggacgc gagtacgacc atgcattcgc gccctggttt ctttcagcaa
    17581 tgcctgagct ttactgcgtg cggcttcagc ttcccctttg gcgtcgctgc cccaggactt
    17641 caatacagat tcgaggctgt cagctaattg attgacatca ttctggatat cctgcacgcc
    17701 atcatcaacg tcgttgcggt tcggtcggtt aaacatatga tcccccatta gaattcgatg
    17761 taattttcag tgtagaccat aattttctta actgaggttc atcgcggctt tatggacttt
    17821 tctgaaagcg ttgtgaatgc gcaatcaggt aaggtaggcc cgcggttgat aataacgagg
    17881 atcaaatatg tatttacgac cagacgaggt ggcgcgcgta cttgaaaaag tcggttttac
    17941 tgtcgatgtg gtaacccaga aagcttatgg ttaccgccgt ggggaaaatt atgtctatgt
    18001 taatcgcgaa gcgcgaatgg gacgtaccgc actggtaatt catccaacat taaaagaacg
    18061 tagttcgaca ctggctgaac ccgcttccga tattaaaact tgcgatcatt atcaacaatt
    18121 tccgctctat ttagcaggcg agcgacacga gcattacggt atcccgcatg gcttcagttc
    18181 gcgtgttgcg cttgaacgtt atttgaatgg tttatttggc gaagccagtt aaagacgcga
    18241 ctggcgatgc cagtcgcgaa aagaagagtc aggcttttac ctgctggtaa tggctgactt
    18301 taaacagtcg gcggcaataa tcgaggaagt agccataaac cgcccccatc aacatcgaaa
    18361 caacgatgtt tgaactgacc gccgccataa tctgatgcca gtctgcgccc accactaaca
    18421 agatcgccac atacaccggt gactggaacg tcacataagc caggatatca gccagatttt
    18481 ttatccagcc cgacgggcta actttgcgtg ccgcgcgcat aaatagatcg cggtacatac
    18541 cgtatggcca tgcaattaag atgttcaccg gaatcgctac caatctggaa taaaaagact
    18601 gttcgaagct cattccggag aggaaaactt caatacacat gttcacgaca gaacagtaaa
    18661 caaccatcgc gaacgtatct gcaactgcat gacgcaagcg tgactgcggt gagaacatgc
    18721 taatgctcct taataaaagc gtaatgggat gttaatggag atgaaagcag tggatctcat
    18781 tgccatctgc attttcgcat agcgtatatc gctggctatg actattcaat tagtgaaaaa
    18841 tttttattta gtcagttttt gtagataaaa tattccgtaa cggctcgttt tttgtacatt
    18901 gtggattttt acatgctgat atgatttatt ttcaattaaa tcaattagat atgaccttgc
    18961 ctttaatttc actaatgaaa gtttgggggt gaggtaacgc tataagcgta gagaattcgg
    19021 tgtatgtcgt gaccgtgtca tttacagcat ttttgatgcc agaaaatgta cattaacagc
    19081 agaa
//
LOCUS       DAJVRF010000075        18840 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJVRF010000075 DAJVRF010000000
VERSION     DAJVRF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.075"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(250..2121)
                     /gene="ppiD"
                     /locus_tag="OQN13_002408"
     CDS             complement(250..2121)
                     /gene="ppiD"
                     /locus_tag="OQN13_002408"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS1615743.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2313..2585)
                     /gene="hupB"
                     /locus_tag="OQN13_002409"
     CDS             complement(2313..2585)
                     /gene="hupB"
                     /locus_tag="OQN13_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCS1615744.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2794..5148)
                     /gene="lon"
                     /locus_tag="OQN13_002410"
     CDS             complement(2794..5148)
                     /gene="lon"
                     /locus_tag="OQN13_002410"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCS1615745.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5336..6610)
                     /gene="clpX"
                     /locus_tag="OQN13_002411"
     CDS             complement(5336..6610)
                     /gene="clpX"
                     /locus_tag="OQN13_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCS1615746.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6736..7359)
                     /gene="clpP"
                     /locus_tag="OQN13_002412"
     CDS             complement(6736..7359)
                     /gene="clpP"
                     /locus_tag="OQN13_002412"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCS1615747.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7605..8903)
                     /gene="tig"
                     /locus_tag="OQN13_002413"
     CDS             complement(7605..8903)
                     /gene="tig"
                     /locus_tag="OQN13_002413"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCS1615748.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9247..9564)
                     /gene="bolA"
                     /locus_tag="OQN13_002414"
     CDS             complement(9247..9564)
                     /gene="bolA"
                     /locus_tag="OQN13_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCS1615749.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9869..10447
                     /locus_tag="OQN13_002415"
     CDS             9869..10447
                     /locus_tag="OQN13_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1615750.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10491..11966
                     /gene="ampG"
                     /locus_tag="OQN13_002416"
     CDS             10491..11966
                     /gene="ampG"
                     /locus_tag="OQN13_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCS1615751.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12427..13374
                     /gene="cyoA"
                     /locus_tag="OQN13_002417"
     CDS             12427..13374
                     /gene="cyoA"
                     /locus_tag="OQN13_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCS1615752.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13396..15387
                     /gene="cyoB"
                     /locus_tag="OQN13_002418"
     CDS             13396..15387
                     /gene="cyoB"
                     /locus_tag="OQN13_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCS1615753.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15377..15991
                     /locus_tag="OQN13_002419"
     CDS             15377..15991
                     /locus_tag="OQN13_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCS1615754.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15991..16320
                     /locus_tag="OQN13_002420"
     CDS             15991..16320
                     /locus_tag="OQN13_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCS1615755.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16332..17222
                     /gene="cyoE"
                     /locus_tag="OQN13_002421"
     CDS             16332..17222
                     /gene="cyoE"
                     /locus_tag="OQN13_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCS1615756.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17407..17718
                     /locus_tag="OQN13_002422"
     CDS             17407..17718
                     /locus_tag="OQN13_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCS1615757.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17702..18244
                     /locus_tag="OQN13_002423"
     CDS             17702..18244
                     /locus_tag="OQN13_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1615758.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18300..>18840)
                     /locus_tag="OQN13_002424"
     CDS             complement(18300..>18840)
                     /locus_tag="OQN13_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCS1615759.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4216 a         4952 c         4579 g         5093 t
ORIGIN      
        1 cgtcggtttc gccacagaag ctgccgccag cgcgctatga gacattccgg cacaggccag
       61 ggacagggta atgagcagtg ctttaattcc gtgtttcata ctgttttctc cttgtttgtt
      121 aacagtggaa tcaccttaac gggagtgaaa acagtaaaca aatggcaatg ttcagaaatg
      181 gaaaaggccg cgaaagcgac cttttcttgt tacagctgca acgcattttt ttgcgaggcg
      241 gcttcaggat tattgctgtt ccagcgcatc gccaattttg attttcgcct ctttacgcag
      301 gttactcatc agagcttcaa agacgatttg tgcgttgttc tgggtgatac cctgcaccat
      361 cgcttttttc tgatcttccg gcattgaacc ttgtttcact tcatccagcg ccagcagaac
      421 cacattacct tgcatatcgg tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg
      481 cagtgcaaac gccgcctggc taatcgggtc acgaccggaa cggcttaagg ttttcggctc
      541 gccaaatttc agaccggcag cctgcatagc ttccgcccct ttgccggctt tcaaatcaac
      601 cagcagtttc tcagcatcca ctttcgcctg ttgttcagct ttgttgtgct gaaccagtgc
      661 cttaacttgt tcctgaacat ctgccaacgg tttcaccgct tccggtttgt gctcgctgat
      721 gcgcagcacg aatgcgcggt cgccgtctac ggtgatgatg tcagagttga tgcccggcgc
      781 gccgttttca cctaccagac cgccgttaaa gatagcgtcg gcaaccggct tgaagttcaa
      841 ctcttccggc aggttatctt tgctgaacca acccgtctga gtggctttaa cgccggcagc
      901 ttgctctgca ccggccagag actcggtgtc gttgcttgcc gcatcgctca ctttctgctg
      961 cagcgcatag tacgcatcga gggctttttc gtgtttcact ttcgccgcaa tgtcgtcacg
     1021 tacttcgtct aacgatttca ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa
     1081 accgaccgaa gatttgatga caccagacag ttggcctttt tctttcagac cagcattttt
     1141 cagttcgtcg gggatagtgg catcttctaa ccaacccata tcgccgccgt tacgggcaga
     1201 gataatatca gcagattttt ctttggctaa tgcagcaaaa tcaccgcctt tattcagctc
     1261 atcaagtacc gctttcgctt catcttcagt tttggtctgg atgatgctgt agcgggtacg
     1321 ctgcggctgg gtgaattgat cctgatgctg atcgtagtag ctctggatat ccgcatcgct
     1381 aaccggttgc tgcatcgttg cggcatccag cttgatgtaa ctcacgcgga attgttccgg
     1441 cgtcatgaaa ttgtttttgt tttgttcgta gtagctggca atctcctgtt cggtcacagg
     1501 ctgcttcgcc gccagcgcgt taacatcgat agtcgcctca cgcaccacgc gttgttgcgc
     1561 gaccagtgcc gccagctcgt cggtttcacc tttcagcata aaatcggtac cggcaacacc
     1621 gttaatcagc tgttgggtag tgagctggtt acgcagcgcc tgggcgtact gatcggcggt
     1681 catccccatc tggttgagga taccgttata gcggctgtta tcaaatttgc cgtcaacctg
     1741 gaaggctggg gtcgcgaaaa tcgcctgttt aacctgctca tcactgatac ccagtttcag
     1801 ctcacgagcg tactgatcca acagcgcctc gtcgatcaga cgattcagca cctgttgacg
     1861 cagggttttc atatagcctt cgttcgctgc cagctcggag tattgatcgc ccagctgttg
     1921 ctgcatgcga ttacgctcgc tgttaaaggc gttctcgaac tgcccacggc tgatttcctg
     1981 gtcattcact tttgcggcgt agttattgcc tccgccaatc aggtaaccac tcacgccggt
     2041 caatatgaac gacacgataa tgataccgaa aataatcttg agcacgagac tgtttgcagc
     2101 cgtgcgtaag ctgtccatca tggtgtaaca acactccgct gtagatgact gtgtacctca
     2161 cgcaacgtat cgatgcgcgc aaaggcctat tgtgacaaga aacgggggca attgtcagcc
     2221 cacaagacac agaaagtcac cgaatacaaa taaaaaaggc acatcagtag atgcgccctt
     2281 gaacttcgtc acatccccac tggggacaac gcttagttta ccgcgtcttt cagtgcttta
     2341 cctgcacgga agctcggtac tttagcggca gcgatggtga tctctttacc ggtctgcggg
     2401 ttgcggccag tacgggcagc acgctcttta acggcaaaag taccaaaacc taccagtgct
     2461 acatcatccc cttctttcag agattcagtt acggaagcaa taatagcatc taacgcacgg
     2521 ccagccgcag ctttagagat atcagcccct gcagcaatct tgtcgatcaa ttgagattta
     2581 ttcactcttc tcttcctctt tataatttat atcgcacttg aatccttcaa ggtacgaacg
     2641 cgcagcagtt atatcaggcc agccatcccc ttacaacacc ccttaataag ggcaagcccg
     2701 atccgccatc taacttagcg agacaaaaaa aggctggcaa gcccgaatta gcctgccagc
     2761 cctgttttta ttagtgcatt ttgcgcgagg tcactatttt gcagtcacaa cctgcatgcc
     2821 agacggttca ttttgcagcg ccagagtcag aacttcctca atgcgcttca caggatgaat
     2881 gtccagatcg gcaattacgt tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg
     2941 aattagcact gttttaatcc cgccgcgatg cgctgccagg agtttttctt tcaaaccacc
     3001 gatcggcagt acctgaccac gcagagtgat ctcaccggtc attgccacat cggcacgaac
     3061 cgggttaccg gtcaggcaag aaaccagcgc ggtgcacata gcaataccgg cactcggacc
     3121 atctttcggc gtcgcacctt ccggtacgtg gacgtggatg tcacgttttt cgtaaaaatc
     3181 agggttgatc cccagttttt ccgcacgcgc acgaaccacc gttaacgccg cctgaataga
     3241 ctcctgcatc acttcgccga gcgaaccggt ataggtcagt ttgcctttac ccggaacaca
     3301 tgcggtttca atggtcagca agtcaccgcc cacttccgtc cacgccagac cggttacctg
     3361 accgacacgg ttttcgttat cagcgcgacc atagtcgaaa cgctgaacac cgaggtagtc
     3421 atgcaggtta tcgccgttaa tttcgatatg ttttaatgac ttatcgagca gtaactgctt
     3481 aaccgctttg cggcacagtt tggagatttc acgctccaga ccacgcacgc ccgcctcacg
     3541 ggtgtagtaa cgaataatgc cgataatggc gctatcgtcg acggtcagct cacctttttt
     3601 cagtgcatta cgttcaatct gcttcggcag caggtgacgt ttggcgatgt tcagtttttc
     3661 atcttcggta taaccggaga ggcgaatcac ttccatacga tccagcagcg gtgccggaat
     3721 gttcatggag ttcgacgtcg cgacaaacat cacgtcgctg agatcgtaat ccacttccag
     3781 gtagtggtcg ctgaacgcta cgttctgctc tggatccagc acttcaagca gtgcagaggc
     3841 cggatcgcct cgcatgtcag aagacatttt gtcgatctca tcgagcagga acagcgggtt
     3901 tttcacgccc actttcgcca ttttctggat cagtttaccc ggcatagaac cgatgtaagt
     3961 acggcggtga ccacggattt ccgcttcatc acgcacgccg cccagcgcca tacggacata
     4021 tttacgcccg gtggctttgg caatggactg accaagagag gttttaccta cccccggcgg
     4081 ccctaccagg cagaggatcg gtcccttgat tttgttgaca cggctttgaa ccgcaaggta
     4141 ctcaaggatg cgatctttca cgcgctccag accataatgg tcggtatcaa ggatttcctg
     4201 cgcctgacgc aggtcttttt tgaccttgct gcgcgcattc cacggtacct gtaccatcca
     4261 gtcgatataa ccacgcacta cggtcgcttc tgccgacatc ggagacatca ttttcagctt
     4321 ctgcaactct gcttccgctt tctcttttgc ctctttcggc atcttcgccg cgtcgatttt
     4381 gcgcttcagg gcttcgtttt cgtccggcgc gtcgtccatt tcaccgagtt ctttctgaat
     4441 agctttcatt tgctcgttca gatagtactc acgctgggat ttctccatct gctttttaac
     4501 gcggttgcga atgcgtttct caacctgcag cagatcgatt tccgattcca tcattgccat
     4561 cagatattcc agacgttcgt taacgtcgga catctccaga acggactgtt tgtcagccag
     4621 tttcagcggc atatgtgcag caatggtatc cgccagacgc gccggatcgt cgatgctatt
     4681 cagcgacgtc agcacttctg gtgggatttt tttgttcagc ttgatgtagc cttcgaactg
     4741 gctgattgca gtacgcacca gcacttcctg ttcccgctca tcaatggtcg gcgactccag
     4801 atactccgcc ttcgcagaaa agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg
     4861 ctgtaacccc tcgaccagca ctttgacggt gccgtcaggc agtttcagca tctgcaatat
     4921 agaggccacg gtcccgacgg tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc
     4981 tttctgcgcg accagcataa tttttttatc atggtccatc gccgcttcca gacaacggat
     5041 agatttttcc cgcccgacaa ataaggggat gaccatgtgc ggataaacca ccacatcgcg
     5101 cagcggcaat acggggattt caatgcgttc agaacgctca ggattcatag agctctctct
     5161 tagtttaatt tccgccaggt aatcagatga cacgactgtg cttcacgcca tctattaaca
     5221 tgtacgtcag tatatgggga tgtttccccc acattcaacg ccgagaatag aggaaaaatt
     5281 aaaggggaga taaaatcccc cctttttggt taactaattg tatgggaatg gttaattatt
     5341 caccagatgc ctgttgcgct tccggcttac cataaatcag caacggtttg ctttgaccat
     5401 caattaccga ctcgtcgata accacttttt cgacgtcttc catggacggc agatcgtaca
     5461 tggtatcgag cagtgcggct tctacgatgg aacgcaggcc acgggcaccg gttttacgcg
     5521 ccatcgcttt cttagcgata gcatccagcg cctcgtcacg gaattccaga tccacgcctt
     5581 ccagattaaa cagcgcctga tactgcttgg tcagggcgtt tttcggctct ttgaggatct
     5641 gaatcagagc ttcttcgctc agttcattca acgttgctac aaccggcaga cgaccaatga
     5701 actcagggat aagaccaaac ttgatcagat cttccggttc aacctgcgcc agcagctcgc
     5761 cttcgcttgc tttgtcggac ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg
     5821 tttctacacg gtgggaaatc actttatcca gaccggcaaa cgcaccgcca caaataaaca
     5881 ggatcttaga ggtatcaacc tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg
     5941 gtggaacagc agctaccgta ccttcgatca gtttcaacag tgcctgctgt acgccttcac
     6001 cggaaacgtc tcgggtaatg gacgggttgt ctgacttacg agaaatcttg tcgatttcat
     6061 cgatgtagac aatcccgcgc tgcgctttct gtacgtcgta atcgcacttt tgcaacagct
     6121 tctgaatgat gttttcaacg tcttcgccca cataaccggc ttcggtcagc gtggttgcgt
     6181 cggccatggt gaacgggacg tccagcaggc gcgccagcgt ttcagccagc agcgttttac
     6241 cggaaccggt cggaccgatc agcagaatgt tacttttgcc caactcgaca ccattgctgg
     6301 tatcgccgtt gcgcagacgt ttgtaatggt tgtataccgc gaccgccagc acttttttcg
     6361 cctgttcctg gccgataacg taatcgtcca ggtggttgcg aatttcatgc ggcgtcggta
     6421 gcgcactgcg ttcacgatgc ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac
     6481 ataaatcaac acattcgtcg cagatataca cggatggacc ggcaatcagc ttgcgcactt
     6541 catgctggct tttgccgcaa aaagagcaat acagcaattt gcctgagcca tctttgcgtt
     6601 tatctgtcat gggtcaaaac ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg
     6661 ccattctcag gcgcaagccg cttacagcgt tgtgccgccc tggataagta tagcggcaca
     6721 gttgcgcctc tggcatcaat tacgatgggt cagaatcgaa tcgaccagac cgtattccac
     6781 cgcttcaggg gcggaaagga agcgatcgcg ctcggtatca cgttcaatct gttctaatga
     6841 ttgacccgta tgaagcgcca taagttcatt catgcgccct ttaactttca gaatttcacg
     6901 ggcatgaatt tcgatatcgg tcgcctggcc ctggtagccg cccaacggtt ggtgaatcat
     6961 cacgcgcgaa ttcggcaggc aaaaacgttt accttttgcc cctgcggtca gcaagaaagc
     7021 gcccatcgag gccgcctggc ccatacagat ggtgctgaca tcaggcttga taaactgcat
     7081 ggtgtcatag atagacatcc cggcagtgat caccccgcct ggggagttaa tgtacagata
     7141 gatatctttt tctgggtttt ccgcttctag gaacagcatc tgcgccacaa tcaggttcgc
     7201 catgtggtct tcaacctggc cagtcagaaa aatgacgcgt tccttaagta gacgagaata
     7261 gatatcaaaa gagcgctcac cgcgtgaggt ctgttcaatg accatcggca ccagcgccat
     7321 atggggtgca aagttatctc gttcgccgct gtatgacatt tccgtctcct ggataaaatt
     7381 gaaaaaacct gctgtaccga ttgtaacctt atggacggat tatcagctag tccctgtgtt
     7441 atgggtacgg catcaccata tttcaagcat aacaatcttt tgttgttacg ctaacaccga
     7501 aagaggcttt tcgcacggtt ccatgcaaaa ttcatgacaa aaaaaagccc accacctggc
     7561 ggtgacgggc ctttgtgcga atttagcgcg ttatgctgcg taaattacgc ctgctggttc
     7621 atcagctcgt tgaaagtggt ttctttttca gtcactttcg ctttcgccag tacagcttca
     7681 acagcctgtt cttccagagc aacattgcgc atgttgtcca tcagttcttt gtttttgctg
     7741 tagaactcga taacttcttt cggatcttcg tacgcagaag ccatctcttc gatcaggcct
     7801 ttcacgcgct cttcgtcagc tttcagttcg ttggtgcgga taacttcgcc cagcagcagg
     7861 ccaacaacta cgcggcgttt agcctgttct tcgaacagtt cgcgcggcag ttccagagct
     7921 tgtttttcgt tgccaccgaa acgctgtgca gcctggcgac gcagaacgtc gatttcgctg
     7981 tcgatcagcg cagccggtac gtcgatgtcg ttagctttta ccagaccttc gatcgcctga
     8041 gacttaacgc ggttacggat ggcgctcttc agctcgcgct ccatgttttt acgcacttca
     8101 gcgcgcagac cttctacgga accatcttca acgccgaaac gtttgatgaa ttctgcagtc
     8161 agttccggca gttcacgctc ttcaactttc ttcaggttga tagcgaattt cgctgcttta
     8221 cctttcagat tttctgcgtg gtattcttcc gggaaggtca cgtcgatggt gaactcttcg
     8281 ccagctttgt ggcctttgat accgtcttca aagcccggga tcatacgacc ctggcccatc
     8341 gccagtacga aatcagacgc tttaccgcct tcgaactctt cgccgtctac agaaccggtg
     8401 aagtcgatgg ttacgcggtc ttctgcttca acggcgccgt ctttttcttt ccaggtcgcc
     8461 tgctgtttac gcagagtatc cagcatgccg tcaacgtcag cgtcggtcac ttcaacgatc
     8521 ggtttttcaa cttcgatcgc ttccagaccc tgcagttcaa cttccggata aacttcaaac
     8581 tctacagagt aggtgaagtc ttcacccagc ttgtattcgc ccggaacata agtcggtgcg
     8641 ccagccggat tgattttttc tttaatgatg gcgtcaatga agttacggct catcaggtca
     8701 cccagaacgt cctggcgtac agacgcgcca taacgctgag caacgatatt cattggcact
     8761 ttgcctttgc ggaagccgtc aatacgtact tttttcgcaa cgttgaccag ctcgctttta
     8821 acagcggtct cgatgctgtc agcagcgata gtaatcgtta cacggcggcc aaggccttga
     8881 gtggtttcaa ctgaaacttg catcttgtta cctcaaaaaa tcacagtgct cggtcaactc
     8941 ttaccccgca agcacaggtt gttggcttcg cggaaccggg atgttcttat aatcaatcac
     9001 attccctgtt accagaatca tcccgaaagc attcaaatag gacgcggcat tatagcggca
     9061 tcacttttat gagtcgagaa cggttattgc gcgctgtcgc gtattttttc acaattcccg
     9121 ggcaattttc gtctgaatgc tgggcaaaaa gcgcaaaatc cagacaaaac aaaacggccc
     9181 gcaggccgtt tttcataaaa tctttagcaa catactggaa aagcgccgac agttgcaaat
     9241 gcgtttttac gcgatgcttc ctgctccacg acagggagga gaggcaaaga cggtgtcctg
     9301 caacccttcc cactccttaa tagtgtaagt atgcagagcc agcgcatgaa cggtagtaga
     9361 gagttcctcc gctaaagtac tgtaaatcat tcgatgacga ttcagaaaac gttcacccgt
     9421 aaaacgatcg ctgaccagca caactttaaa atggctttca gagccggctg ggacattgtg
     9481 acgatagctt tcatccacta cttcgaggaa tacgggttgg aacgccgccc ttaatttttc
     9541 ttctatccgc tcacgtatca tcatgaaata tctcctccga caacgctgag atgtcatcca
     9601 tccctttaaa tactagccgc ttttaccgtt tccattgcag cttaacaaca aatatttagg
     9661 tttcgtcctg gtttttccgt caagcgtatg aagtttactg atttaaagat tgaaacacag
     9721 gcctcgcatc agcgtttctt ttaccagggg tgattatttc gccaacatga tgaatttaca
     9781 tgccttaccc acttcccctc gccgttggca atgttatgat ggcgggaatt tttaccaaac
     9841 acgcttaaga gtctctgaga gcccgaacat gttcaaaaaa atcctcttcc cgttagttgc
     9901 tctgtttatg cttgcaggat gcgcaaaacc gccaacaact attgaagttt ccccgacgat
     9961 tacactgcca cagcaggatc caagcctgat gggcgtcacc gtaagcatta atggtgccga
    10021 tcagcgtacc gatcaggcgc tggcaaaagt cacccgcgat aatcaaatcg ttaccctgac
    10081 cgcctcccga gatctgcgtt tcctgctgca agaagtgctg gaaaaacaga tgaccgcgcg
    10141 tggttacatg gttggtccga atggcccggt taatctgcaa atcatcgtta gccaactgta
    10201 tgctgacgtg tcccagggca acgtgcgcta caacatcgcg accaaagcgg atatcgccat
    10261 catcgctacc gcgcagaatg gcaacaaaat gaccaaaaac tatcgtgcca gctacaacgt
    10321 tgaaggtgcg ttccaggcct ccaacaaaaa tatcgccgat gcggttaaca gtgtgctgac
    10381 cgataccatc gctgatatgt ctcaggacac cagcatccac gaattcatca agcagaacgc
    10441 gcgttaattt ctgccctctg gcccggtgca aaccgggcct gtagacgccc atgtccagtc
    10501 aatatttacg tatttttcaa cagccgcgtt cagccatatt gctgatcctg ggttttgctt
    10561 ccgggctacc gctcgccctg acatccggta ccttacaggc ctggatgacg gtcgagaata
    10621 ttgatctcaa aaccattggt ttcttctctc tggtaggcca ggcttacgtt tttaaattcc
    10681 tctggtcacc gctgatggac cgctacacgc ccccattttt tgggcggcgg cgcggttggc
    10741 tgctcgccac gcaaatcctg ttattagtcg ccattgcggc gatgggtttt ctcgaacctg
    10801 gcacccaact ccgctggatg gcggcactgg cggtggtgat cgctttttgc tcggcctccc
    10861 aggatattgt cttcgatgcg tggaaaaccg atgtgctccc ggcagaagaa cgtggtgcgg
    10921 gcgcggcaat cagcgtgctg ggttaccgtt tagggatgct ggtttccggc ggcttagccc
    10981 tgtggctggc agataaatgg ctgggctggc agggcatgta ctggttgatg gcggcgctgt
    11041 tgatcccttg tattatcgcg acgttgcttg caccagaacc gaccgacacc attcctgtgc
    11101 caaaaacgct ggaacaagcg gttgttgcac ctctgcgaga tttctttggt cgcaataatg
    11161 cctggcttat tttgcttctt atcgtgctgt ataagctggg cgacgcattc gccatgagcc
    11221 tgacaaccac gtttttgatt cgcggcgtcg ggtttgatgc gggtgaagta ggcgtggtta
    11281 acaaaacgct tggcttactg gcgaccattg tgggcgcatt gtacggtggg attttgatgc
    11341 agcgcctgtc gctgttccgg gcgctgctga ttttcggcat tttacaaggt gcgtctaacg
    11401 ctggttactg gctgctgtcg attactgata agcatctcta cagcatgggc gcagccgtct
    11461 ttttcgaaaa cctctgtggc gggatgggca catcagcctt tgtcgcactg ttaatgacgc
    11521 tgtgtaataa gtcattttcc gctactcaat ttgccctgct ctcagcgctt tccgctgtag
    11581 gacgagttta tgtcggcccc gtggcgggtt ggtttgttga agcacacggc tggtcgacat
    11641 tctatctatt ctccgtcgcc gctgccgtac cggggcttat tttgctgctg gtttgccgcc
    11701 agacgcttga atatacacga gtaaatgaca actttatctc ccgtaccgaa tatccgacag
    11761 gttatgcctt tgccatgtgg acactggcgg cgggcgtcag cctgttggcc gtgtggttac
    11821 tgctgttgac gatggacgcg ctggatttga cgcacttctc tttcctgcct gctctgctgg
    11881 aagtcggggt tttagtcgcc ctttctggcg tcgtgcttgg tggtttgctg gattatctgg
    11941 ctctacgaaa aacgcatctg acgtaatctg taaatattat ttagctgtag taatcactcg
    12001 ccgtaattat tacggctaaa taaacatcag tatcgcttaa taatatttgt tcttttgtgc
    12061 ggcttagcgt ttggaaatta ttgacgcaat ttatgaattc tattttttct tacacgattc
    12121 agctaatgag tctttatttt ccccccaccc aattgtcacc ataatgataa ttatttgtta
    12181 aacaattgtt ttattcaaga ttggttatac caattgcccg tccaaactcc ttctgcactc
    12241 ccctttcgtt ataacgccct tttgcaacag cttcttaaaa tcaacttgat atgttttgca
    12301 acatatgtaa cctgacagcc aaatccaagt aacaggaatt taatcatgtt tacagtaatg
    12361 taacctcccc gtaaaatgcc cacacacttt aaacgccacc agatcccgtg gaattgaggt
    12421 cgttaaatga gactcaggaa atacaataaa agtttgggat ggttgtcatt atttgcaggc
    12481 actgtattgc tcagtggctg taattctgcg ctgttagatc ccaaaggaca gattggtctg
    12541 gagcaacgtt cactgatact gacggcattt ggcctgatgt tgattgtcgt tattcccgca
    12601 atcttgatgg ctgttggttt cgcctggaag tatcgtgcga gcaataaaga tgctaagtac
    12661 agcccgaact ggtcacactc caataaagtg gaagctgtgg tctggacggt acctatctta
    12721 atcatcatct tccttgcggt actgacctgg aaaaccactc acgctcttga gcctagcaag
    12781 ccgctggcac acgacgagaa gcccattacc atcgaagtgg tttccatgga ctggaaatgg
    12841 ttcttcatct acccggaaca gggcattgca accgtgaatg aaatcgcttt cccggcgaac
    12901 actccggtgt acttcaaagt gacctccaac tccgtgatga actccttctt cattccgcgt
    12961 ctgggtagcc agatttatgc catggccggt atgcagactc gcctgcatct gatcgccaac
    13021 gaacccggta cttatgacgg tatctccgcc agctacagcg ggccgggctt ctcaggcatg
    13081 aagttcaaag ctattgcaac accggatcgc gccgaattcg accaatgggt cgcaaaagcg
    13141 aaacagtcgc cgaacaccat gtctgacatg gctgcgttcg aaaaactggc cgcgcctagc
    13201 gaatacaacc aggtggaata tttctccaac gtgaaaccag acttgtttgc cgatgtgatt
    13261 aacaagttta tggctcacgg taagagcatg gacatgaccc agccagaagg tgagcacagt
    13321 gcacacgaag gtatggaagg catggacatg agccacgcgg aatccgccca ttaaaggggt
    13381 tgaggaagaa taaagatgtt cggaaaatta tcacttgatg cagtcccgtt ccatgaacct
    13441 atcgtcatgg ttacgatcgc tggcattatt ttgggaggtc tggcgctcgt tggcctgatc
    13501 acttacttcg gtaagtggac ctacctgtgg aaagagtggc tgacctccgt cgaccataaa
    13561 cgcctcggta tcatgtatat catcgtggcg attgtgatgt tgctgcgtgg ttttgctgac
    13621 gccattatga tgcgtagcca gcaggctctt gcctcggcgg gcgaagcggg cttcctgcca
    13681 cctcaccact acgatcagat cttcaccgcg cacggcgtga ttatgatctt cttcgtggcg
    13741 atgcctttcg ttatcggtct gatgaacctg gtggttccgc tgcagatcgg cgcgcgtgac
    13801 gttgcgttcc cgttcctcaa caacttaagc ttctggttta ctgttgttgg tgtgattctg
    13861 gttaacgttt ctctcggcgt gggcgaattt gcgcagaccg gctggctggc ctatccaccg
    13921 ctatcgggaa tagagtacag tccgggagtc ggtgtcgatt actggatatg gagtctccag
    13981 ctatccggta taggtacgac gcttaccggt atcaacttct tcgttaccat tctgaagatg
    14041 cgcgcaccgg gcatgaccat gttcaagatg ccagtattta cctgggcatc actgtgcgcg
    14101 aacgtactga ttattgcttc cttcccaatt ctgacggtta ccgtcgcgtt gttgaccctg
    14161 gatcgctatc tgggcaccca tttctttacc aacgatatgg gtggcaacat gatgatgtac
    14221 atcaacctga tttgggcctg gggccacccg gaagtttaca tcctgatcct gcctgttttc
    14281 ggtgtgttct ccgaaattgc ggcaaccttc tcgcgtaaac gtctgtttgg ttatacctcg
    14341 ctggtatggg caaccgtctg tatcaccgtg ctgtcgttca tcgtttggct gcaccacttc
    14401 tttacgatgg gtgcgggcgc gaacgtaaac gccttctttg gtatcaccac aatgattatc
    14461 gccatcccga ccggggtgaa gatcttcaac tggctgttca ccatgtatca gggccgcatc
    14521 gtgttccatt ctgcgatgct gtggaccatc ggttttatcg tcactttctc ggtgggcggg
    14581 atgactggcg tgctgctggc agtaccgggc gcggacttcg ttctgcataa cagcctgttc
    14641 ctgattgcgc acttccataa cgttatcatc ggcggcgtgg tcttcggctg cttcgcaggg
    14701 atgacctact ggtggcctaa agcgttcggt ttcaaactga atgaaacctg gggtaaacgc
    14761 gcgttctggt tctggatcat cggcttcttc gttgccttta tgccgctgta tgcgctgggc
    14821 ttcatgggca tgacccgtcg tttgagccag cagattgacc cgcagttcca caccatgctg
    14881 atgattgcag ctagcggtgc agtactgatt gcgctgggta ttctctgcct cgttattcag
    14941 atgtacgttt ctattcgcga ccgcgaccag aaccgtgacc tgactggcga cccgtggggt
    15001 ggccgtacgc tggagtgggc aacctcttcc ccgcctccgt tctataactt tgccgttgtg
    15061 ccgcacgttc acgagcgtga tgcattctgg gaaatgaaag agaaaggcga agcgtataaa
    15121 aagcctgacc actatgaaga aattcatatg ccgaaaaaca gcggtgcagg tatcgtcatt
    15181 gcagctttct ccaccatctt cggtttcgcc atgatctggc atatctggtg gctggcgatt
    15241 gttggcttcg caggcatgat catcacctgg atcgtgaaaa gcttcgacga ggacgtggat
    15301 tactacgtgc cggtggcaga aatcgaaaaa ctggaaaacc agcatttcga tgagattact
    15361 aaggcagggc tgaaaaatgg caactgatac tttgacgcac gcgactgccc acgcgcacga
    15421 acacgggcac cacgatgcag gcggaaccaa aatcttcgga ttttggatct acctgatgag
    15481 cgactgcatt ctgttctcaa tcttgtttgc tacctatgcc gttctggtga acggcaccgc
    15541 aggcggcccg acaggtaagg acattttcga actgccgttc gttctggttg aaactttctt
    15601 gctgttgttc agctccatca cctacggcat ggcggctatc gccatgtaca aaaacaacaa
    15661 aagccaggtt atctcctggc tggcgttgac ctggttgttt ggtgccggat ttatcgggat
    15721 ggaaatctac gaattccatc acctgattgt taacggcatg ggtccggatc gcagcggctt
    15781 cctgtcagcg ttctttgcgc tggtcggcac gcacggtctg cacgtcactt ccggtcttat
    15841 ctggatggcg gtgctgatgg tacaaatcgc tcgtcgcggc ctgaccagca ctaaccgtac
    15901 ccgcatcatg tgcctgagcc tgttctggca cttcctggat gtggtttgga tctgtgtgtt
    15961 cactgttgtt tatctgatgg gggcgatgta atgagtcatt ctaccgatca cagcggcgcg
    16021 tcccatggca gcgtaaaaac ctacatgaca ggctttatcc tgtcgatcat tctgacggtg
    16081 attccgttct ggatggtgat gacaggagct gcctctccgg ccgtaattct gggaacaatc
    16141 ctggcaatgg cagtggtaca ggttctggtg catctggtgt gcttcctgca catgaatacc
    16201 aaatcagatg aaggctggaa catgacggcg tttgtcttca ccgtgctaat catcgctatc
    16261 ctggttgtag gctccatctg gattatgtgg aacctcaact acaacatgat gatgcactaa
    16321 gagcggcggt tatgatgttt aagcaatacc tgcaagtaac gaaaccaggc atcatctttg
    16381 gcaacctgat ctcggtgatt gggggattcc tgctggcctc aaagggcagc attgattatc
    16441 ccctgtttat ctacacgctg gttggggtgt cactggttgt ggcgtcgggt tgtgtgttta
    16501 acaactacat cgacagggat atcgacagaa agatggaaag gacgaagaat cgggtgctgg
    16561 ttaaaggcct gatctctcct gctgtctcgc tggtgtacgc caccttgcta ggctttgctg
    16621 gctttatgct gctgtggttt ggcgcgaatc cgctggcctg ctggctgggg gtgatgggct
    16681 ttgtggttta tgtcggcgtt tatagcctgt acatgaaacg ccactcggtc tacggcacat
    16741 tgattggttc gctctccggc gctgcgccgc cggtgattgg ctactgtgca gtaaccggcg
    16801 agttcgatag cggcgcagcg atcctgctgg ctatcttcag cctgtggcag atgcctcact
    16861 cctatgccat cgccattttc cgctttaagg attatcaggc ggcaaacatt ccggtattgc
    16921 cagtggtaaa aggcatttcg gtggcgaaga atcacatcac gctgtatatc atcgcctttg
    16981 ccgttgccac gctgatgctc tctcttggcg gttacgctgg gtataaatat ctggtggtcg
    17041 ccgcggcggt gagcgtctgg tggttaggta tggccctgcg cggttataaa gttgctgatg
    17101 acaggatctg ggcgcgcaag ctgttcggct tctctatcat cgccatcaca gccctctcgg
    17161 tgatgatgtc cgttgatttt atggtgccgg actcgcatac gctgctggct gctgtttggt
    17221 aacacaacct ctctattaaa aaggtgctac ggcacctttt ttattgtctg agatcagaga
    17281 tacggctaat aaccgtatgt cttgacagag tgagcagatg aacataaatt aatcatacgg
    17341 ttgatagccg tattatgtat gagatcgact ccctacgatt tcattcagtt aatcaaagag
    17401 gtcataatgc ctggaaaaac agccactcta cccgatgtcg ataaaacgct gaaaaatgcg
    17461 cgggtcgaac tcaaaactag tcccgacgcc aaaaacaaac ttcgtgaagc tgcgcaagcc
    17521 gttggggtag atttaagcgc ttttattttg agcgctgcta tggaacgcgc cgaaagtgtg
    17581 cttgataacc aacgtcgccg tgagctttcg aatcaaagct gggaactgat gaaccaactc
    17641 atcgctgaac ctgctcaacc gacgctcgcc ctcaaggcgt taatgaaaag gaaaaacagc
    17701 gatggtcgac aagcatgaag agattactct gcccatagtc ctctcctgta attatcagtc
    17761 tgatattact tatcctgggc aaaaacagtt tgattgcggt aaccctgtta tcgataaatt
    17821 tgtacgcgca tcgctaaaga aaagtgtgcg taatagcgac tgtgcggcta aagcacttat
    17881 tgacagacaa agtggtgaac tgatcggcat ctgtactttt acggcatatt cgctggaaaa
    17941 acaacgcgtt tctggcgtcc ttcagggttc acaaccttca gaaattggtg ttgtcagatt
    18001 agtcatgttg ggggtagcac ggaagtatca aaagcggggc tttggtcagg acctactatg
    18061 tgattttttt gaacatgtaa aaataattca ccaggcatta ccaattaaag gggtttatct
    18121 tgatgctgac cctgccgcca ttaattttta tgctcgtctc ggctttgttc agctttcagc
    18181 gacaccaaat gcttttggtg ctgtacctat gtttttggcg attcagcata ttctcgcggc
    18241 ttagattatt atcgccaccc gccagggcaa gttttgcctt agccgggggc tgagcagcgt
    18301 taccactttt gttcaattgc ccccgctttc agcaactcca catccttgcc gttaatggtt
    18361 gccgttgcgg caacgattgt ggtccttaca gttgcacaag attttcgctc aaattccggt
    18421 ttataaagaa taagagacga attactggta aatgaaccaa ataggtaatc agtaaagcta
    18481 atattcagcc ggtttaaacc gaaaggcgcg aagccaaccg tttggttaat ctccttaccg
    18541 gatgggcgac gaatgctgaa tgtcacgcga agattggcca cttcgcctaa tttattggtg
    18601 gtggcgaaga ggctatccat gcaggaaaaa cctttatcta aaaaattatc tcgcatccca
    18661 gaacgggtaa ccgtaatcac gggtaacgta tcgtcaaccg tttttctgtc aatcatgtct
    18721 tttcgacaag tagccagaaa gtcttccggg ccaccataaa tgtggacacc tttatcaaga
    18781 tattccttcg ctttcttaca atctacctca acatactgac cattggcgta cataatgccc
//
LOCUS       DAJVRF010000076        18523 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJVRF010000076 DAJVRF010000000
VERSION     DAJVRF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.076"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..1710
                     /locus_tag="OQN13_002425"
     CDS             <1..1710
                     /locus_tag="OQN13_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCS1615760.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1711..3360
                     /locus_tag="OQN13_002426"
     CDS             1711..3360
                     /locus_tag="OQN13_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCS1615761.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3365..4141
                     /locus_tag="OQN13_002427"
     CDS             3365..4141
                     /locus_tag="OQN13_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCS1615762.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="OQN13_002428"
     CDS             complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="OQN13_002428"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HCS1615763.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5430..6752)
                     /locus_tag="OQN13_002429"
     CDS             complement(5430..6752)
                     /locus_tag="OQN13_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HCS1615764.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="OQN13_002430"
     CDS             complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="OQN13_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HCS1615765.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="OQN13_002431"
     CDS             complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="OQN13_002431"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HCS1615766.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="OQN13_002432"
     CDS             complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="OQN13_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HCS1615767.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="OQN13_002433"
     CDS             complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="OQN13_002433"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HCS1615768.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            11632..14481
                     /gene="rcsC"
                     /locus_tag="OQN13_002434"
     CDS             11632..14481
                     /gene="rcsC"
                     /locus_tag="OQN13_002434"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCS1615769.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="OQN13_002435"
     CDS             complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="OQN13_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCS1615770.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="OQN13_002436"
     CDS             complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="OQN13_002436"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCS1615771.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEKIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         4526 a         4535 c         4867 g         4595 t
ORIGIN      
        1 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac
       61 attcagcaat ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc
      121 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc
      181 atgctcggcg gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta
      241 caaagtagcg aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga
      301 ccatggggtg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
      361 tcgcttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
      421 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
      481 ggcaatggtg acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag
      541 gcgctcggcg taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag
      601 caggcggata acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg
      661 aatctgccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
      721 aaagatgaag atttctctga ggaagacatc cgcgatatca atgacagcct gatcctcgat
      781 acaccggaat ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa
      841 aaagcgcagt tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg
      901 gcaaacagca atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct
      961 acccagacgg ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg
     1021 ctggccatga actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg
     1081 cctgcgggcg catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt
     1141 ggtcagggtg tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag
     1201 gtccgctggc gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa
     1261 cgccagttgt atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg
     1321 accagcaatg agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag
     1381 caggatgcag ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt
     1441 gagcgcacaa catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa
     1501 ttgctggaaa aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa
     1561 ctgacgggaa cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt
     1621 ctgcctcctg ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg
     1681 agtgaatgga ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg
     1741 gcgttggtta ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt
     1801 gcgcagcacg atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta
     1861 cctgactcgc tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg
     1921 gaggataccc atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt
     1981 tattgttgtc aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg
     2041 ctctattttg cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa
     2101 cagcgtcagg cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta
     2161 acggttaaat cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa
     2221 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt
     2281 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt
     2341 ggttttgctg gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc
     2401 aaaacggtac taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgacgcag
     2461 gttgccagtg ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc
     2521 acagcggaaa agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
     2581 acgtttacta atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc
     2641 gaaaagggca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt
     2701 ctggatcgcg aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata
     2761 cgtacttttt tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc
     2821 agcgccacgc agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct
     2881 tggacgcagg atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc
     2941 gaagggacac tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac
     3001 cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca
     3061 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg
     3121 cgtatattac aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta
     3181 gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
     3241 acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
     3301 cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa
     3361 tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag
     3421 tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg
     3481 cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
     3541 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
     3601 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
     3661 tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag
     3721 cccggatggg caaagccggc aaaaaatgca gttttactca acgtcgggta taggaacgat
     3781 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
     3841 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg
     3901 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc
     3961 agttcacggg cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt
     4021 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
     4081 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
     4141 attaatgggg ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg
     4201 ggtgttttta ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca
     4261 atgcacagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg
     4321 accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg
     4381 acattcactt tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca
     4441 aatgcttcat tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac
     4501 gctttttgcg tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg
     4561 gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc
     4621 ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt
     4681 cctgctttat cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc
     4741 gggaattcgt cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg
     4801 atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc
     4861 gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta
     4921 atccccatat gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa
     4981 acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct
     5041 aaactcatat tttccatacc ccccgccaca atgctcttcg cctgacctgc ctgaattgcc
     5101 tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg
     5161 cacaccgttt ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac
     5221 ccggcttgca acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca
     5281 cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt
     5341 gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt
     5401 ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg
     5461 acgccggaga agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg
     5521 gcgattgcca gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg
     5581 taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc
     5641 acgaaaggtc cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt
     5701 gcagaactaa aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat
     5761 tcggtaatga gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag
     5821 gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa
     5881 gccattggcg ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg
     5941 atggtgatgt tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg
     6001 ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc
     6061 ggtagctgct tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg
     6121 tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca
     6181 gtgatgaaaa tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata
     6241 tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca
     6301 ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg
     6361 actcgccggg cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg
     6421 atgacacaag cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt
     6481 gcggcggagg cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga
     6541 ccggtaacga tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca
     6601 ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat
     6661 gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa
     6721 cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg
     6781 cggcgacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg
     6841 caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca
     6901 gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg
     6961 aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat
     7021 ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg
     7081 cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta
     7141 gcgcaaatga catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc
     7201 cgccagcgtt caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg aggaagccgt
     7261 tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta
     7321 aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa
     7381 tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc
     7441 accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc
     7501 taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg
     7561 ataggtcagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg
     7621 cagtgggcgt tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc
     7681 tacgacggtg ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg
     7741 aatttgctcg attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat
     7801 gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc
     7861 attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt
     7921 caggtcgcga acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc
     7981 cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa
     8041 tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca
     8101 ggcaatagca aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa
     8161 tatgaaggga taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg
     8221 cgcactgtgc aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc
     8281 gtattcctgg agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg
     8341 ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt
     8401 aatttcctct tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca
     8461 gactggctga cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat
     8521 cacgacggcg cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc
     8581 ggtaagcagt gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact
     8641 gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg
     8701 cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc
     8761 ttttaccatt gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat
     8821 ggtctgatgg ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc
     8881 ttgtagtacc agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg
     8941 ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa
     9001 cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc
     9061 ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct
     9121 ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat
     9181 gtccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc
     9241 ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat
     9301 taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag
     9361 cgcttcgacg gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt
     9421 ctcatggctg cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc
     9481 catcaacacc acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt
     9541 cgcacaatgt gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt
     9601 atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc
     9661 gggttgatcg gtaaaataag cgtgaaggtt gcgccgtagc ccggcaaact ggcgacgcga
     9721 atatcaccct gatgggcatt aatgatgcgt tgacttaacg ccagaccaag cccggttcct
     9781 gaggctttgg tggtgaaaaa gggatcgaag atcttttttt gcagcgagag atcaatgcca
     9841 cagccgttgt cctctatcga aatggcctgt tgtgagtcgc tgtattgcca ggtttgaatg
     9901 cgaattttcc ctcgtgcgct gatagcctgg acggcattga tcaggatatt cagtagtacc
     9961 tgtttgagca gttcacgatc ggcgttaatc gggcttaatt cattatccag ttcgcttatg
    10021 aagtcgaccc gcgcttgtac gccggcggtt tgtaccagta ccagagtttc ttcaaccaat
    10081 gcattgaggc tgacttgttg ccattgactg tggcgtggac gtgaaaattc gagcaattgc
    10141 tgaataactt tgttaattga atcgatttct ttgagtacta cggacagata ttcctgatgt
    10201 attgggtcac tggtttgttg gcgcaagatc tgtacataac cacgaatagc cgttaacgga
    10261 ttacgtactt catgcgcgac gccagccatc agctcaccca gtgtggcgag gcgttctgct
    10321 tgcgccatgc ggcgctgggt ttctttgcgg gcagttaaat cagagaaaat caccaaagca
    10381 cctatcattt caccgtgcgt gttatgaata cgactggtag tgacactgag ttcaatggtg
    10441 cggtcacgac ctggaaaact gatctccagc gccacatgtt cggtgccatg ttccagcgta
    10501 tccagtactg gactgtagaa ctgagtattg tcgaacaaca tggagtaagg ctgccctacc
    10561 agttcatggc gttgatagcc agtgataact tctgctgctg ggttcatggt ggttacatca
    10621 ccctggcggt caatggcaat gacgccatcg gcagcgtttt caataatcag atcgttaagt
    10681 gtccgcgttt cacgcagtgc ctgggcgagg ttattaacac tctgactgat ttgccccatt
    10741 tcaccgggca attgtggtaa tcgagtggga atattttgtg ccagagtcga gaggccatcg
    10801 gtgatgatat caatattggc gctcaggcga cgggagaaaa ggacaatcaa tagcaggctt
    10861 atcagcaaat cggcggtgag cacaatgata atcctcacat ccattttcca ggcctggcgg
    10921 cgaatatctt cggttaattc attggcccag atatagccga ggatttcacc attacgctca
    10981 atggggagca ttgaattcaa aatatcgccg cgcacctgcc tgcctgaata aaccaaaggg
    11041 gtatttgtac gcatgacttc gcgaccaggg tgatctgcgg caatggtaac gccgacatta
    11101 ttctgatata gcgctgaagg cgcgtaggtg attatcgcat ccagcatttt gttgtaataa
    11161 ccagcaccta tgccagggaa ggcgtgagtg atattttcgg taatgggggc aagttctgca
    11221 tttaatgcgc ggatacgctc ctcacgtggt aagtcgatgt agagatcata gcgatcgcct
    11281 agtgcctgat taagcaggtt gaccacggca gaaagttttt tctctttttc agataagact
    11341 gctgaacgtc cttccgtttc tacgatataa ccaatagtaa gcgttgggac aatgaccatc
    11401 aggattgcca tcaggatcat ttgattgcgt aagcggcgtg gataaatcca cttcatataa
    11461 tgcatgctga aatccactag tcttgtccgg tatatgacga ttatcagagg ttaaggtgat
    11521 gatttctcgg cggtgtatca tattccagag aagagagaac attgcggtaa cacgctttta
    11581 ccgctacctt aaccacactc catcggtcac ctgaggcgga gcttcgcccc tttgaaatac
    11641 cttgcttctt ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc attggcgtta
    11701 gtgctctggc tgttgattgc tttttcatcc gttttttaca tcgttaatgc gttacatcag
    11761 cgagaatcgg aaattcgtca ggaatttaat ctgagttccg atcaggctca gcgctttatt
    11821 caacgcacct ctgatgtgat gaaagagctg aagtacatcg ccgaaaatcg cttatcggca
    11881 gaaaacggtg tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc tgcgtttgaa
    11941 ccgctgtttg ccgactccga ttgttccgca atgagtaaca cctggcgagg ttctctggag
    12001 tcattggcgt ggtttatgcg ctactggcgc gataattttt ctgcggctta cgatctaaac
    12061 cgggtatttt taatcggcag cgataacctc tgcatggcca atttcggtct gcgtgatatg
    12121 ccagtggaac gcgataccgc gttgaaagct ttgcatgaac gcatcaataa atatcgaaat
    12181 gcaccacaag atgatagcgg cagtaacctc tactggatca gcgaaggtcc gcgccctggc
    12241 gtcgggtatt tttacgcgtt gacgccagtt tatctggcga accggttgca ggcgcttttg
    12301 ggtgtcgagc agaccatccg gatggagaac tttttcttac cgggtacgtt gccgatgggg
    12361 gttaccattc ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc agaaagtaaa
    12421 attaagggcg atcctcgctg gatgcaggaa cgctcctggt ttggctatac ggaagggttc
    12481 cgggagctgg tgctgaagaa aaatctgcca ccctcatcgc taagcatcgt gtattcggtg
    12541 ccggttgata aggtgctgga acgcattcgc atgttgatcc ttaacgcaat tttgctgaat
    12601 gtgcttgccg gagctgcatt gtttactctc gcacggatgt acgagcgacg tattttcatt
    12661 ccggcggaaa gcgacgccct gcgactggaa gaacatgagc agttcaatcg caagattgtc
    12721 gcctccgcgc cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa tattttaagt
    12781 aacgaactgg cgcataccta tctcaatatg cttacgcatg aggaccgcca acgactgacg
    12841 caaattatct gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa caataccaat
    12901 ctgcaaatca gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat ttgtgtgctg
    12961 gtggatgttt cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc acaagcagcg
    13021 gaacaggcga gccagtcaaa atcgatgttc cttgccaccg tcagtcatga gctgcgaacg
    13081 ccgctgtatg gcattatcgg taacctggat ctgttgcaaa ccaaagagtt accgaaaggc
    13141 gtcgatcggc tggtgacggc aatgaacaac tcttccagcc tgttgttgaa aattatcagc
    13201 gatattctcg atttctcgaa gattgaatcg gaacagttga agatcgaacc gcgtgagttt
    13261 tcaccgcgtg aagtgatgaa ccacatcacc gccaactatt taccgctggt ggtacgcaag
    13321 cagttaggct tgtactgctt tattgaaccg gatgtgccag tggccttaaa tggcgacccg
    13381 atgcgtttac agcaggtcat ctccaacctg ttgagtaacg ccataaaatt caccgatacc
    13441 ggctgtatag ttttgcatgt ccgcgctgat ggcgattatc tctctatccg tgttcgcgat
    13501 accggcgtgg ggattccggc gaaagaagtg gtgcgcttgt ttgatccctt cttccaggtc
    13561 ggaacgggcg tacagcgaaa cttccagggg accggtctgg gtctggcgat ttgtgaaaaa
    13621 ctgatcagca tgatggacgg cgatatctcg gtagattcag aaccgggaat gggcagccag
    13681 tttaccgtgc gtattccgtt gtacggcgct cagtacccgc agaaaaaagg cgtggaaggg
    13741 ttgagtggta aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca gttcctggaa
    13801 accagtttgc agcgcagcgg catcgtcgtt acaacatacg aagggcagga accgactccc
    13861 gaagatgtgt taattactga cgaggtagtg agtaaaaaat ggcagggtag agcggtagtg
    13921 accttctgtc gtcgccatat tggtattccg ctggagaaag cgccagggga gtgggtacac
    13981 agtgtggctg ccccgcatga gctaccggca ttgttggcgc gtatttattt gatcgagatg
    14041 gagagcgacg atcctgctaa cgctctgccg tcgacggaca aagcggtcag cgataatgac
    14101 gatatgatga ttctggtcgt ggatgatcat ccgattaacc gacgtctgct ggcagatcag
    14161 ttgggatcgt tgggctatca atgtaaaacc gcgaatgatg gtgtcgatgc gcttaatgta
    14221 cttagcaaga atcatattga tatcgtgctt agcgacgtca acatgccaaa tatggatggt
    14281 taccgcttga cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat cggagtaact
    14341 gctaatgcgt tggctgaaga gaagcagcgg tgtctggagt ccggtatgga cagctgcctg
    14401 tcgaagccgg taacgctgga tgtgataaaa cagacgctga cggtatatgc cgagagggtc
    14461 aggaaatcgc gggaatcgta ggacggataa ggtgtttatg ccgcatctgg cattcagtgc
    14521 aaatgccaga tgcgacgctg acgcgtctta tctggcctac tttgatgtaa tctgttgaaa
    14581 taatgggaat cgtaggacgc ataaggcgtt tacgccgcat tcggcaatcg gtgcaaatgc
    14641 cagataagac actaacgcgt cttatctggc ctacaggtga ttagtcttta tctgccggac
    14701 ttaacgtcac tgaagagaga taattcagca gggcgatatc gttctcgaca cccagcttca
    14761 tcatcgcaga tttcttctgg ctactgatgg ttttaatact gcggttcagc tttttagcga
    14821 tctcggtcac caggaagcct tccgcaaaca ggcgcagaac ttcactctct tttggcgaga
    14881 gacgcttgtc accgtaacca ccagcactga ttttttccaa caggcgagaa acgctttccg
    14941 gggtaaattt cttccctttc tgcagcgcgg cgagagcttt cggcagatcg gtcggtgcac
    15001 cttgtttcag cacgatccct tcgatatcca gatccaatac cgcactaaga atcgccgggt
    15061 tgttgttcat agtcagaaca atgatcgaca ggcttgggaa atggcgcttg atgtacttga
    15121 ttaaggtaat gccatcgccg tacttatcgc caggcatgga gagatcggta atcaacacat
    15181 gcgcatccag tttcggcagg ttgttgatca gtgctgtaga gtcttcaaat tcgccgacaa
    15241 cattcaccca ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact atcggatggt
    15301 catcggcaat aattacgttc atattgttca tgtaataggc taccttgcta cagcaagctc
    15361 ttgacataac tgtcaatgtc gctgatgtat ttttctattc ctggaacatc cttctcacga
    15421 atcagatgtt ccagcgtttc acataactgc ttgccgggta ccagatttag catggcaaat
    15481 acgcctttaa gacgatgagc cgtttgggct aacgcagcaa agtcactggt tgctgcttca
    15541 gtatacagcc tcttaacatc atccggtact gtgtctacaa agagcgcata atagccgctg
    15601 gcatggagtt gcgcattttc atctccgccc agaggcgatt ctgtcacctc ttcctgcgcc
    15661 agttgcactt caattaattg taagaccgct tcctgcatag cgttgctcat attgaagttg
    15721 acgcacaatt gaccaggccc aatttcccgt acgccagact catcatcgct taaaagcaag
    15781 ccagaggcag taagattaga cggattatcc gttaaaaaga tatcataatc ttgactaatt
    15841 aatctttcat cgggtgtgat acaggttgca ccccaatttt ctaactggcg agtgacaata
    15901 ttccgaattt ctgccgaagt aacatccacc attacgcaga catcatccag taaacgctct
    15961 tcttcctctt caacttccgg gccagcggcg agcattttga tatgcacaga gtagcgtgta
    16021 ccaagcccat cccgcgtttt gatgtttaaa tgaccgccca gtttacgtgc cagttgatcg
    16081 ctcagccaga atgccagcgg gtccgccttt ccataacgat cgttttgggt ctggttgata
    16141 aacgggaagt gcaaattatc catttcatga atacttacgc cttctccggt gtccagaatg
    16201 cggaatgtca ggcggtcttc agaggactca tcctgatcaa cctcaagggt gatttttccc
    16261 aattgcgttg aggtcacggc atattgcatc agtagcagca aaatacgtcg taaggcatcg
    16321 cgatcgccgc ggcgcatatc gtgtgctttc agatgattgt taatcagcag ttgcagacct
    16381 ttacgcttga tggcaggcaa cactgaaggc acaacttcat caattaaatc ctgcacggag
    16441 aacagcaccg tctcactttt ccagctatcg tccgcaagca tgttcgctaa ctgtatttca
    16501 tcgaccagcc gcaccagcac atctgcctga ttcgccagtt gtttgctttc cggggcgttg
    16561 agtttagccg cgctctccgc cagggactgt gcaggttctt tcagcgcatc gccaatgttt
    16621 ttcataaagg tcatccggcc ctgctggttt ttctcataca gacgctgcgc ctgcttgagt
    16681 ttcttgttta ccagcacttc gcgatcctga tcgcgaataa tgaaaatttg tgtgcgcggc
    16741 gcgacctggc tgcggaacat gcggatctca tacagctcgt tattgatcgt cgcctgaata
    16801 atcccctgat gctgttccgc catggtggtg atgttttgca gattcaaatg cggcagcaaa
    16861 tgatcggcaa ttttgttact tatgacagtg cggttcgatt cctgatcgtg aaccagcagg
    16921 ccgagcggca gcagtgagac tatcttttca ttgattgccc gtaaaatgcg caattcgtta
    16981 ttgaccgccg tgttgggtag actttctgta ctgcgactgg agaaatggcg gaatgtggta
    17041 tagccaaata acgccagcgc cagcaaaccg atgttcagca gcagtggcag cagaatgttt
    17101 tgcaacgtat ccagcaataa ggtgccataa ggaacctgcc agaccaggcg catatcggta
    17161 gagttgagtg ccgaggagat ttcaatcttc gtactgttaa agtggatact gacgctatcc
    17221 gttccttctt tctcattatc attgtctccc gttgccgtcg tgtctggctc aaggcggaaa
    17281 ctgtccagcg gcatacccgg tgggatcaaa tcattaatcg gcagatcaaa agccacgacc
    17341 gttgccagat gtcccggctg gttgaacgtg gtacgcaagg taaagtaatg accgttctgc
    17401 caggccaggc ggcgcatgtt agaaaaactt tcgcgttcat cgagggcgtt ggcctgttgc
    17461 aacatttctg cacgacgtga atcaacaatg tcactgacgg tcgattcttt aaatccggag
    17521 gtgagatctt tgaggggcag ggttgagatc agtaccagac tgttatcctg accattcagg
    17581 taatacatcg accacggtac attttctgca ccccacaatg tatccagata agtggacatc
    17641 cgctgagtca tctcaagcgt tgagttgtcg tgagagccaa agattaacgc ttccgttttg
    17701 cggcgtggtt tctccagata gtagacatcc tgtttcaggc gcgtctcttg taaaccttcg
    17761 ccggaggagg gggaggtcgt cgcggcaatg ttgtcgtaga tctgccaggt cacgtaacgc
    17821 caggtatcga cgcgcttttg gattgcgtgg gtaatgtcga caatctggta acttttatct
    17881 ttcagccagg cgttaacggc gctttgtacc attaccccca tcgtcaccag taacacaatg
    17941 atcaacagta aaaagaagcg ggtaatgctc cccggtagga gtgaaaagcg ggtcgtggcc
    18001 gttgtctctt tctgacgcat tcgtgtttat gacctgttaa aacttcgcga agtgataggg
    18061 cagtataaag ggtacagtgt gaaattccag actcttacct gattgatgat tctctattct
    18121 cccgtgatat ttatcatcct gcattggggg aaatagatgt acaagcgcca ttttgctgta
    18181 caaataacct acaaaaagcc caacgaaatt aattgcaaat aaacgaagat tgctggaaat
    18241 tatgctgatg ttaattattt gtgaaatagt tgacaagagt tatagttttt ctgtggtagc
    18301 acagaataat gaaagtgtgt aaagaagggt aaaaaaaacc ggatgcgagg catccggttg
    18361 aaataggggt aaacagacat tcagaaatga atgacggtaa taaataaagt taatgatgat
    18421 agcgggagat attctagttg cgagtgaagg ttttgttttg acattcagtg ctgtcaaata
    18481 cttaagaata agttattgat tttaaccttg aattattatt gct
//
LOCUS       DAJVRF010000077        18161 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJVRF010000077 DAJVRF010000000
VERSION     DAJVRF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18161
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.077"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            383..688
                     /locus_tag="OQN13_002437"
     CDS             383..688
                     /locus_tag="OQN13_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1615772.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            795..1439
                     /gene="gfcB"
                     /locus_tag="OQN13_002438"
     CDS             795..1439
                     /gene="gfcB"
                     /locus_tag="OQN13_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HCS1615773.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1436..2182
                     /locus_tag="OQN13_002439"
     CDS             1436..2182
                     /locus_tag="OQN13_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCS1615774.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2182..4278
                     /locus_tag="OQN13_002440"
     CDS             2182..4278
                     /locus_tag="OQN13_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCS1615775.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4324..5463
                     /locus_tag="OQN13_002441"
     CDS             4324..5463
                     /locus_tag="OQN13_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCS1615776.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5451..5897
                     /gene="etp"
                     /locus_tag="OQN13_002442"
                     /gene_synonym="yccY"
     CDS             5451..5897
                     /gene="etp"
                     /locus_tag="OQN13_002442"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCS1615777.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            5917..8097
                     /gene="etk"
                     /locus_tag="OQN13_002443"
     CDS             5917..8097
                     /gene="etk"
                     /locus_tag="OQN13_002443"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCS1615778.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8212..9510)
                     /gene="appA"
                     /locus_tag="OQN13_002444"
     CDS             complement(8212..9510)
                     /gene="appA"
                     /locus_tag="OQN13_002444"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCS1615779.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9590..9682)
                     /gene="cbdX"
                     /locus_tag="OQN13_002445"
     CDS             complement(9590..9682)
                     /gene="cbdX"
                     /locus_tag="OQN13_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCS1615780.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(9695..10831)
                     /gene="appB"
                     /locus_tag="OQN13_002446"
     CDS             complement(9695..10831)
                     /gene="appB"
                     /locus_tag="OQN13_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS1615781.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(10843..12387)
                     /gene="appC"
                     /locus_tag="OQN13_002447"
     CDS             complement(10843..12387)
                     /gene="appC"
                     /locus_tag="OQN13_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCS1615782.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12521..13378)
                     /locus_tag="OQN13_002448"
     CDS             complement(12521..13378)
                     /locus_tag="OQN13_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCS1615783.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13435..13773)
                     /gene="hyaE"
                     /locus_tag="OQN13_002449"
     CDS             complement(13435..13773)
                     /gene="hyaE"
                     /locus_tag="OQN13_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCS1615784.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(13770..14357)
                     /locus_tag="OQN13_002450"
     CDS             complement(13770..14357)
                     /locus_tag="OQN13_002450"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCS1615785.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14354..15060)
                     /gene="hyaC"
                     /locus_tag="OQN13_002451"
                     /pseudo
     CDS             complement(14354..15060)
                     /gene="hyaC"
                     /locus_tag="OQN13_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(15079..16872)
                     /gene="hyaB"
                     /locus_tag="OQN13_002452"
     CDS             complement(15079..16872)
                     /gene="hyaB"
                     /locus_tag="OQN13_002452"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCS1615786.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNTSPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(16869..17987)
                     /gene="hyaA"
                     /locus_tag="OQN13_002453"
     CDS             complement(16869..17987)
                     /gene="hyaA"
                     /locus_tag="OQN13_002453"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCS1615787.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4448 a         4836 c         4706 g         4171 t
ORIGIN      
        1 ttataatatc agttactttt ccacaaagaa actaatccat ttaagcgaat atctctgcac
       61 ctaatagtta taaatgccat ttaacagcca gttatagtac cacttgaaac tatattacca
      121 acaataatta atagctttcg taacaatttc cttatcttta atttgtattt tgctaagatt
      181 tatcttatgc tcacaagtcg tatttccaga ggggatcgtt aataatctta tatttccctc
      241 tgcgcacctt tcctacccgc agtatcagta tatagtgcaa gcggtatgag gaaagggaga
      301 tttcacaagg atgttatact caaagtagac aatcctgatc tcaagcgtac gtattgtcca
      361 tgcgatgaat aaaggaaaag ttatgaaaca caagctttct gcaatcctta tggccttcat
      421 gttgacgacg ccagctgcgt ttgctgcctc tgaagcaaca aacgggaccg aggcaacgac
      481 gggtactact ggcaccacaa cgaccactac cggcgcaacc acgactgcta ctaccactgg
      541 tggcgtggct gctggcgctg tcggcacggc gacagttgtc ggtgtggcaa cggctgttgg
      601 tgtggcgacg ttggcagttg ttgcagcaaa tgacagtggc gatggcggtt ctcataacac
      661 ctcaaccacc acgtccacta cacgctaata ctgctttaac gcgtcgaaat taaagttaaa
      721 tgatacccgg ttgcgatatt cgtaccgggc tttagagatt tgcatttttt taattaacct
      781 ttcaaggatt aaaggtgcgc cctcttattt tatcgatttt cgcactattt cttgcgggat
      841 gtacgcacag ccagcaaagt atggtcgata catttcgcgc cagccttttc gataatcagg
      901 atatcaccgt agcggatcag cagatccagg cgttgcctta ttccacgatg tatttacgcc
      961 ttaatgaagg gcaacgaatc tttgtggtac tgggatatat agaacaagaa caaagcaaat
     1021 ggttatccca ggataacgcc atgctggtta cccacaatgg acgtctttta aaaaccgtca
     1081 aacttaataa taatctgctg gaagtgacaa attccgggca ggaccctctg cgtaacgcgc
     1141 tggcaataaa agatggcagc cgctggacgc gcgatattct ctggagtgaa gacaaccatt
     1201 ttcgctctgc gaccctgagt tctacttttt cctttgctgg attagagacg ctgaatattg
     1261 cgggtcgcaa tgtgctgtgt aatgtctggc aggaagaggt gacttccacg cggccagaaa
     1321 aacagtggca aaacacattc tgggtcgatt cggctactgg ccaggttcgt caaagtcggc
     1381 aaatgttagg cgcaggggtt attcccgtag aaatgacgtt tcttaaaccc gcaccatgaa
     1441 taaattacag tcgtatttca ttgccagcgt actttacgta atgacacccc atgcctttgc
     1501 gcaaggaacg gtgactattt atctgcctgg cgaacaacag acactttccg ttggccccat
     1561 ggaaaatgtt gtccagttgg tgacacaacc gcaactgcgc gatcgcctct ggtggcctgg
     1621 tgccttgctg accgactctg cggcgaaagc taaagcgctt aaagactacc aacatgtcat
     1681 ggcacagctt gcttcctggg aagcagaagc tgacgacgat gtggcggcaa cgattaaatc
     1741 cgtgcgccag cagttactca atctcaatat cactggtcgc ttaccggtca aactggaccc
     1801 tgattttgta cgagtggatg agaacagcaa tcccccgttg gtcggggact atacgctcta
     1861 taccgtacag cgtccggtga ctattactct gctgggtgcg gtttctgggg ccgggcaatt
     1921 gccctggcag gcaggccgta gcgttaccga ttatctgcaa gatcaccccc gccttgcagg
     1981 cgcggacaag aataacgtta tggtgattac gcctgagggt gagactgtcg tcgcccctgt
     2041 tgccctgtgg aataaaagac atgtggaacc accgcctggc agccagcttt ggttgggctt
     2101 ttctgcgcat gtactgcccg aaaaatatgc tgatttaaat gaccagattg tgtctgtttt
     2161 aacgcaacgt gttcctgact gatgaagaag aattcttatc ttttaagctg cctggccatt
     2221 gccgtctcca gtgcctgtca tgctgaagta ttaacctacc cggatccgct gggttcgtcg
     2281 caatcagact ttggcggcac aggattgttg cagatgccaa atgcgcgcat cgcaccggaa
     2341 ggtgaattca gcgtcaacta ccgggataac gatcaatacc ggttctactc cacctccgtg
     2401 gcgctgttcc catggctgga aggcaccatt cgttatacgg atgtgcgcac acgcaaatat
     2461 agccagtggg aagatttcag cggcgatcag tcatacaaag acaaatcatt cgattttaaa
     2521 cttcgcctgt gggaagaagg ttactggcta ccgcaagtgg cgtttggtaa acgtgatatt
     2581 gctggtacgg gtctgtttga cggtgagtat ctggtggcca gcaagcaagc ggggccattt
     2641 gatttcaccc tcgggatggc atggggctac gccggtaatg cgggcaatat taccaacccg
     2701 ttttgccggg tgagcgataa atattgtcat cgcgcagagt ctcacgatgc gggcgatatc
     2761 agctttagcg atatctttcg tggcccggct tccatctttg gcggcattga gtatcaaacg
     2821 ccgtggaatc ccctgcgtct gaaactcgaa tacgatggca acaattacca gaatgatttc
     2881 gctggcaaac tgcctcaggc aagccatttc aacgtcggcg cagtttatcg cgctgccagc
     2941 tgggcagatc tcaacctgag ttatgaacgc ggtaacacgt tgatgtttgg cttcacgtta
     3001 cggaccaatt tcaacgatct gcgccctgcc ctgcgcgata cgccaaaacc ggcatatcaa
     3061 cctgcgcctg aatctgaagg attgcagtac accacagtag cgaaccaact taccgcgctg
     3121 aagtacaacg caggttttga agcaccggaa attcagctgc gcgataagac gctgtatatg
     3181 tctggtcaac aatacaaata ccgtgattct cgcgaagcgg tcgatcgtgc caaccggatt
     3241 ctggtgaata acctgccgca aggcgttgag aagattagcg tgacgcaaaa gcgcgagcat
     3301 atggcgatgg tgactaccga aaccgacgta gccagcctgc gcaaacagct ggctggtaca
     3361 gcgcctggtc aatcagagca acttcaacaa caacgtgttg aagcagaaga tctttctgcc
     3421 tttggtcggg gctaccgtat tcgtgaagat cgctttagct actctttcaa cccaacactt
     3481 tcacagtcgc tgggcggccc ggaagatttc tatatgttcc agctggggct gatgtccagt
     3541 gcccgctact ggtttaccga ccacctgctg cttgatggcg gtattttcac caatatttac
     3601 aacaactacg acaagtttaa gtcttcgctg ttgcccgcgg actctaccct gccccgcgtg
     3661 cgcacgcata tccgtgatta cgttcgcaat gacgtttatc tcaacaactt gcaggcgaac
     3721 tactttgccg acttaggcaa tggtttctat ggccaggtgt atggcggtta tctggaaacg
     3781 atgtacgccg gtgtcggttc cgagctgctt tatcgcccgc tagatgccag ctgggcgctg
     3841 ggtgtggacg ttaactacgt gaagcaacgt gactgggaca acatgatgcg cttcaccgat
     3901 tattccacgc caactggttt cgtgacggct tactggaacc cgccgacgct caatggcgta
     3961 ctgatgaaac ttagcgttgg gcaatatctg gcaaaagata aaggggcaac gatcgacgtc
     4021 gccaaacgct ttgacagcgg cgtggcggta ggggtatggg cggcaatcag taacgtatct
     4081 aaagatgact acggcgaagg cggctttagt aaaggttttt atatctcgat tccgttcgac
     4141 ttgatgacca ttggacctaa ccgcaaccgc gcggtggttt cgtggacacc attgacgcgt
     4201 gatggtggac aaatgctgtc acgcaaatac cagctctatc caatgacggc agagcgagaa
     4261 gtaccggttg gacaataacc tgacgtaatt cataacatta cattgactga taaatataaa
     4321 ccaatgaaaa agaacatttt taagttttcg gtactgacat tggctgttct gtctttgaca
     4381 gcatgtaccc ttgttcctgg tcagaattta tcgaccagta acaaggatgt cattgaacta
     4441 cctgacaatc agtatgacct ggacaaaatg gtcaatatct atcccgtcac gccaggattg
     4501 attgatcagt tgcgcgccaa accgattatg tcgcaggcta acccggagct ggagcagcaa
     4561 atcgcaaact atgaatatcg tatcgggatt ggtgatgtgt tgatggttac ggtctgggat
     4621 cacccggaac taaccacccc cgcagggcaa taccgtagcg ccagtgatac cggcaactgg
     4681 gttaacgccg atggtgcgat tttctacccc tatattggtc gcctgaaagt ggccgggaaa
     4741 acacttaccc aggtacgcaa cgagattaca gcgcgtctcg attcagtcat tgaaagcccg
     4801 caagtcgatg tcagcgtcgc ggcgttccga tcgcaaaaag cgtacgtcac cggcgaagtc
     4861 agcaaatcgg gtcagcagcc gattaccaat attcctctga ccatcatgga tgcgattaac
     4921 gccgcgggcg gtctgaccgc agatgcagac tggcgcaacg tggtgctcac ccagaatggc
     4981 gtaaagacca aagtgaacct ctatgcgctg atgcagcgtg gcgacttgcg ccagaacaaa
     5041 ctgctgcacc cgggcgatat tttatttatt ccgcgcaacg atgatctgaa agtcttcgtg
     5101 atgggtgaag tgggtaaaca gagcacgctg aaaatggacc gcagcggtat gaccctggcg
     5161 gaagcgctgg gtaacgctga gggtatgaat caggatgtcg ctgatgcgac ggggattttt
     5221 gttattcgtg caacgcaaaa caagcagaat ggcaaaatcg ccaatattta tcagctgaat
     5281 gcgaaggacg catcggcaat gatactcgga acggaattcc agcttgaacc ttacgacatt
     5341 gtttatgtca ctaccgcgcc gctggctcgt tggaaccgtg tgatttctct gctggtaccg
     5401 acgatttccg gggtgcatga cctgacagag acttcacggt ggattcaaac atggcccaac
     5461 taaaatttaa ctcaatcctg gtggtttgta ccggcaacat ttgccgttcg cccattggcg
     5521 agcgcttgct gcgtaaacgt ctgccgggcg taaaagtcaa atcggcaggt gttcatggtc
     5581 tgataaaaca ccctgcagat gcgacagcgg cagatgttgc cgctaatcat ggcgtatctc
     5641 ttgaaggaca tgccggacgt aagctcactg ctgagatggc acgaaattac gatctgattc
     5701 tggcaatgga gtcggaacat attgctcagg ttaccgcaat tgctcccgag gtccgcggga
     5761 agacaatgct gtttgggcaa tggctggaac agaaagagat cccggatccc tatcgtaaaa
     5821 gtcaggacgc atttgaacat gtctacggta tgttggagcg cgccagtcag gaatgggcga
     5881 agcgcctcag ccgataattt gagtttataa aatacgatga caactaaaaa tatgaatacg
     5941 ccaccaggca gcactcagga aaatgagatc gatctgcttc gtctggtcgg cgagttatgg
     6001 gatcaccgta agtttattat cagcgtgacc gcgttattca cgctgatcgc tgtcgcttac
     6061 tcgctgttaa gcacaccaat ttatcaggca gatactctgg tccaggttga gcaaaaacag
     6121 ggcaacgcca ttctcagcgg cctgagcgat atgatcccta actcatcgcc cgagtctgca
     6181 ccggagatcc aactgctgca atcgcgcatg attctcggta aaaccattgc tgaactgaat
     6241 ctgcgcgaca tagttgagca gaagtatttt ccgattgtgg gtcgcggctg ggcgagatta
     6301 accaaagaaa aaccaggtga gctggcgatc agctggatgc atattccaca actgaatggt
     6361 caggatcagc aactgacact cacggttggg gaaaacggcc actatacact ggaaggtgaa
     6421 gagttcaccg tcaatggtat ggtcggccag cgtctggaaa aagatggcgt tgcgctgact
     6481 atcgcggaca ttaaggccaa accaggaaca cagtttgtcc tgagccagcg taccgaactg
     6541 gaagcgatta acgcattgca ggaaaccttt accgttagcg aacgcagtaa agaaagcggg
     6601 atgctggaac ttaccatgac tggtgatgat ccccagttga ttactcgtat tctgaacagc
     6661 atcgctaaca actatttgca acagaatatc gctcgccagg cggcgcagga ttcacaaagc
     6721 cttgaattct tacagcgcca gttacctgaa gtgcgcagcg agctggacca ggcggaagaa
     6781 aaactcaacg tttatcgcca gcagcgcgat tcggttgacc ttaacctgga agccaaagcc
     6841 gttcttgagc agattgtgaa cgttgataat caactcaatg agctgacctt ccgcgaggca
     6901 gagatctccc agctgtataa gaaagatcac ccaacttatc gtgcgctgct ggaaaaacgc
     6961 cagacgctgg agcaagaacg caaacgcctg aataagcggg tatcggcaat gccttccacc
     7021 caacaggaag tgttgcgttt aagtcgtgac gtagaagcgg gccgtgcggt atatctgcaa
     7081 ttacttaacc gccagcagga gttgagtatt tcgaaatcca gtgccattgg taacgtgcgg
     7141 attatcgacc cggcagtcac tcagccgcag ccagtgaaac cgaaaaaagc gttgaatgtg
     7201 gtgcttggtt ttattcttgg cctgtttatt tctgtgggtg ccgtgctggc gcgtgcgatg
     7261 ttgcgtcgtg gtgtagaagc cccggaacaa ctggaagagc acggcatcag cgtttatgcc
     7321 actatcccaa tgtccgagtg gctggataaa cgcacccgtc tgcgtaagaa aaatttattt
     7381 tctaatcagc agcgccatcg tactaaaaat atccccttcc tggcggtgga taacccggcg
     7441 gattctgctg tggaagccgt acgtgcgcta cgaaccagtc tgcatttcgc tatgatggag
     7501 acggagaata acattctgat gatcaccggt gcgacgccag acagtggtaa aacgtttgtc
     7561 agttcaactc tggcagcggt gatcgcccag tccgatcaaa aagtgttatt tattgatgcc
     7621 gacttacgcc gtggttattc gcataacctg tttaccgtga gtaatgaaca tggcttgtcg
     7681 gaatatctgg caggtaaaga tgagctcaac aaagtgatcc agcattttgg caaaggaggc
     7741 tttgatgtga ttactcgcgg tcaggtgcca cctaacccgt ctgaactgct gatgcgcgat
     7801 cggatgcgtc aattactgga atgggcgaac gaccattacg atctggtgat tgtcgatacg
     7861 ccgccgatgc tggcggtgag tgatgccgcg gtcgtggggc gttctgttgg caccagcctg
     7921 ctggttgcgc gttttggctt gaacaccgcc aaagaggtga gtttgtcaat gcagcgtctg
     7981 gaacaggcag gcgtcaatat taaaggcgct atcctcaatg gtgtgattaa acgcgccagc
     8041 accgcttaca gttacggcta taactattac ggttatagtt actccgagaa agagtaaaac
     8101 tgaaagtggc cggatgcggc gtgaacgcct tttccggtct acgcattaga cagttcttcg
     8161 tttgtcatca gcctcagagc attcaggtaa ctgaatgctc ttttttatgc attacaaact
     8221 gcacgccggt atgcgtgctt cattcacgat ttgcgtaaaa cctgccaacg aacacatgcc
     8281 ctgcgcattt cgctcttcac atcctgccag ggtcagtttc acctctccgg gcggcgtatt
     8341 taatgacagc ggcgttttat cacgcatctg ctgtaaagtc tggaagacca gcgaaacctg
     8401 aatccactgg ctgttatcgc ttagccgacg ccagcgttca aacaccagtt caccacctgg
     8461 cggcgtgtta tccggctgac cgggaagcgt ccagttgagc tccagtgcgc cgccgagatt
     8521 tgccagatta gtatcgtgtc cggcgataaa cagcactgaa gtgggtaatg tcacaccata
     8581 cgcctgtttt tgcggtggat ggggcgtcaa cgctgtcttg atcaaatcta ataacggggt
     8641 ggcgcggctg cgggcaacct ctggcgtgcg ttgtagcaaa taaaattgcg cgttatgcaa
     8701 acttagcaag gtgttccact ggtgtgaatc ggtgatcctt ccccaccccg gctccggcat
     8761 tccctgtgct tgttgcagga gaaatatctc cgtcagcatt gatgcgaggc ttaccgcacc
     8821 ggttaatgag acattgtcgg cgctcacctt gagttccgat ggtaatgcct gcgttaatga
     8881 acagctttcg tcctgtttct cacgtttaag gcacaagttt gattgcggaa aattaagcac
     8941 ccgttccagt tcgcgaaacg ccgtttgccg atgcccggta aagtcagcaa ttgaccctcc
     9001 tgccctgctg aggatcgcgt cagtcacgtt cgcgttatcc agttggcaaa cgccagtttt
     9061 tagaggatta aataacggat cgggactgga cgtatcagcc tgggtatgta cggttattgc
     9121 acagtcaggt gccagcccgg cggcgaaggc ttcgcctgtt ttacgggtac gctcgtcgac
     9181 atcagcaata atcgcgacct gaccagactg cgggcagccc tttttcgcca gcaatccgtc
     9241 ggctaccaga cgctggcgtt ggtaatgtcc gagataggcg attagctcac caccgcgcgg
     9301 tgtcagccaa cccagtttta ccggccaggt tggccatgcg tctggggtga catcctgcat
     9361 cagttgcgtg gccttggttg gagcacgcac accatgacga ctgacaatca ccacactttc
     9421 cagcttcagc tccggctcac tctgagcgaa tgcagattgc ggggttaacg gaatcagaag
     9481 agataaaaat gggattaaga tcgctttcat cgatatgttt ccgcttttca tatctgacat
     9541 tattgattgc ctgatgcgat gctaatgcgc cgcatcaggc ctacgttcgt taacttttca
     9601 gacgcgggtc cagccatacc aacacaaggg tggagagcga acacatcaac aaaatgccga
     9661 cgaaccaaag taaataccac attgttcctg ctccttagta caactcgttt tcgttacggc
     9721 ggagagtttc tgttgtcatg cgtccccaca ttttgtagta gctccagaga gtgtagagca
     9781 acacaatggg caaaaatatc agcacgatta ccaacataat gctcagcgtc agctgactgg
     9841 aagtgctgtc ccacaacgtc aggctggaga ttggactcac gcttgacggc atgacaaagg
     9901 ggaacagcgt gatgcctgcc gtgaaaatca cgccaaattg catcaatgac gccattaaaa
     9961 atccccaacc cgggcgacca cgataaatcg ccatcaccgt cagcaatggg cagaagaacc
    10021 ccagcagcgg gaagatccac aaaacgggcg attcgacgaa attattcatc caggcaccag
    10081 gtagcactgc caccagtttc attaacggat tggaaggacc gttagcatcc tgggcgagca
    10141 gtacaaagcc atcaataccg acccacagcc agtaacccgc cagcaaaaag cacagcatca
    10201 ccaacagtgc agcgcgtttg gtggccagct gtgaacgcag atgaatcaca ccaacagttt
    10261 tcagttgtaa ccagacgcca ccttgcaaaa tcaccatccc aaggctgagc aatccgcaca
    10321 ataaagggaa tggcgtcagc agttgccaga agctgccgag atactccacg cgtaattgcg
    10381 gtgtgaaggc aaacggcacg ccgagcaaca agttgccgaa ggcgataccg aagactaccg
    10441 gcggcaccag actgccgatg accagaccgg cgtcccacat tttacgccag cgtgcatcgg
    10501 cgatttttcc gcgataatca aaggccagcg ggcggaagaa cagtgagcac agcaccagga
    10561 tcatcgccac ataaaagccg gaaaacgccg ctgcatacac tctgggccag gcggcaaata
    10621 atgccccacc agcgaggatc aaccagacct ggttgccttc ccagtgtgca ccaacgctgt
    10681 ttatcactat ccggcgttca tcatcattac gcgccaccag cggcagcaga cagccgatcc
    10741 ccatgtcaaa tccgtcggag atcataaaga ccaccaggat cacgccaatc agcagccacc
    10801 agatgaagcg caatgtttca taatcaaaca tgattttccc ctttacccct gttgctgcgt
    10861 cggttgttca ctctgcatcg cgctcggccc cagacgggca tatttctgca tcaggtagac
    10921 ttcggcgatt aagaacaggg tgtaaagccc tacgatcatg atcagtgaga aagccagttg
    10981 tcctgtggtt aaagcggagt gcgcggagta tgtcggtaag atgtcctgta tcgcccacgg
    11041 ctgacgacca aactcggtca taaaccaccc ggcttcaatc gcaatccacg gcaacggcaa
    11101 actccagagc gccattttca gcacccagcg atgctggtcg attttgccac gcagcgtctg
    11161 gacaagcgca atcagcatca ccagtagcgg cagggaacca cagcccacca tgatgcggaa
    11221 actccagaat accggcgcaa cctgaggtat cgcgccacgc atcgccgcct ggtactgtgc
    11281 ggctgtgaca tgattcatat ccggcgcata gcgggagagc aacatgccgt agcccaggtc
    11341 accttccagt tcccggaatg cctgcaacac atgcggctca cgattgcctt gcgatatttc
    11401 ctgcattaac agccaggcca tacgtccgcg ttgcaagcgt gggtaggttt cagccatcaa
    11461 atttttcaga cccggcacgg ttttatctaa tgagtgagtg gcgaggatcc ctaacagcgc
    11521 gggaattttg atggcaaagg cgttacgctc ttgatcctgt tccggccagg caaccacatg
    11581 gaacggtgca ggagcaggtt ccgtttgcca ctccccttcc atcgccgcca gttttaccgg
    11641 ttgtacttgc gcgacttcat acgcagaact gtctccgagt tgtagcgtgc cgataatcgc
    11701 cagagtaccg aagacggaac cgatggcaaa cgagcgtaat gcaacatcgc gctcccgtcc
    11761 gcgcagtaaa taccaggcgc tgatcgccat aataaacatg gccccggtca cgtagcccgc
    11821 cattacggtg tgcacaaatt tcacctggct gaccgggtta aagaccagct cgctgaagct
    11881 ggtcatctcc atacgtaggg tgtcgatatc aaaatgcgca ccggtcgggt attgcatcca
    11941 accgttggca ttcaatatcc acaatgcaga gagatttgaa ccgaacgcca ccagccacgt
    12001 caccagcagg tgctggtatt tattcagccg ctgccagccg aagaagaaaa gcccgacaaa
    12061 ggtggattcg aggaagaagg ccattaatgc ttccatcgcc agcggtgcgc caaaaatatc
    12121 gcccacatag ttggaataga atgaccagtt agtaccaaac tgaaactcca tggtcaggcc
    12181 ggtagccacg ccaagagcaa agttgatacc gaagagctta ccccagaagc gcgtcatatc
    12241 gcggtagatt gttttgccgg tgaccacgta aatggtttcc atgatagcca gcaaaaaaat
    12301 cagccccagg gtaaggggta caaataaaaa gtgatacagc gcggtcagag caaactgcca
    12361 gcgcgataaa tcaatgacat cccacatgcg cactcctgta ggcgaagaga gaaaaataac
    12421 ccgataatgt tagatctccc tctttatcct gaaactctac gaccagaggt attcgccgat
    12481 tgcgatattc ataagactga aagcgatact taccgtcttt ttacgtcggt gcagcttccg
    12541 ccagccactg acatacctcg ctaagccgct gcgcagagtc gaccaaatct tctggcgctg
    12601 ccagcaccac ttccggtatt gggcagattt cataactttc cagtaaagga ccttttaagg
    12661 tgtccgtaca gcgtaaatgc cagacatggc gtaaccccgt ggcgttgata tagctctcgc
    12721 catagccaat ggtacgaatc tgaatatttc ccggcccaca gagacgtgag agaaaaagcc
    12781 gatcagcctc gctgatgggt aattgcgtca gattaatgct gtgcggctgc gcgtcggggt
    12841 tacgtacatg cgccagtaac tcatgcgcca acggtaggcc attcatcagg ccatcaatgg
    12901 gcggcggtaa cagcgaatct gtcggcaaga gattttgcgt ggccgcctgc cacaacgcca
    12961 gcggcgcgca gccggcctcc agtttgtcct ccagcaactt ttcgccgcga cgtttgcgca
    13021 cccgccataa tccgcagaag atcgcctcct gtatttcact ttcgctgtcg tcagcctgct
    13081 gaatgcgcac tgacacttcc ccttccccga gaagcgtatt gataaagtga cgatcgtcgg
    13141 cgttgagatt taacagatcg actgccagca cctcgcccaa cggcggctgg cgttgactta
    13201 gttgatgttg taactcgttt aacagcgcaa tcacctgcgg gctgtgagca cattgctcca
    13261 gggccgccat actcggttca tcattcacct gacagctgat cggcagtgga ttcatgctga
    13321 aactgtcatc gttcggttgc gttcctggcc ccagcagatg gaaaaaagtt tcgctcatga
    13381 ggcacgctcc tgttgtggtt cgacaagccc acgcatcagg tttatcagtt ccgctcacgg
    13441 gtgaatacca ttcagcacac cgcgatagtt tccgccggta aacactaatg tggcgggaaa
    13501 gcgaaagacg ccaaagcgat ctccgatggc ttcgctctgt tcaaggtcag caatcgccac
    13561 ctgccatgtg tagccgggaa actcgcgcag taattcgcca atcattaccg gattatcgct
    13621 gacctctggc gtgcgtttcg ggtcactgct taataacacc acgccgtctg gcgcttgcgt
    13681 aagccagtcg tcaagacgga attcactaac cggcgtccag ccgcgcgcca gcattcgttg
    13741 ccacaacgca tcaaatggcg tgtcgttgct catccctgct cctccagtgt catcctgtac
    13801 tggcgcaagc gaacgccttc gtaattttcc atcgacagac agtcataatt gagacagcgc
    13861 gattcattag ccggttgcgg cacaattccc catgcagcca gctgcgccag cgccgcctgt
    13921 tccgctgcgg gcagttgctc ccgtgccagt tcgctcaggc tgccgccgta gtcgtcgagc
    13981 attgcgggtt gcagaccgac gagggcaata tgtgctggca gatgtccgcg gatatctgcc
    14041 agcgccagca cttcggagaa actgttctgg tgcaggctca ttttcttcgc gctgagataa
    14101 gccggaatac gttctccggc ataggttcgc agcgttccag gttccagccc gtagtcaatg
    14161 gcatcgagaa tcaacagatg gctggcgctt tcgacatacc ccagcaagtt cagtccctga
    14221 gtaccgccat cgacaatctc cacatactcg ggccagtggt aatgggcata caaccgttcc
    14281 gccacccgca cgccgaaacc ttcatcggcc cacagcaggt tgcccagccc cattaccaca
    14341 acgcgttgct cgctcatgaa cgctccttgt tacttatttt gccaaatttg tggctacggt
    14401 agccgttgac catggtggag atcaccgtgt cgtcggacat gatgtcttca cgcagcgcca
    14461 tatagacatg accgatcaca aacgcgccaa tcagccacat ccccagccga tgccagctgt
    14521 gaatgtccat tgagttgcct cccgtccagt agaaaaattc caccacataa cggaacggcg
    14581 caaaaatagc gtactggctg tgttcgctgt acagcgcaaa gccggtgatg atcataaaga
    14641 ccgacatcag gaaatagccg aacatcgccg cctgggcgat ggggttatgg cctatatcgg
    14701 cactcggacg ttttgccaga acagatacca gcggatttca taccacacgc cctgccacca
    14761 gcttttacgc cataccggca cgataaacag ctcgcgggag tatcgattgc caacaaaagc
    14821 ccagtagatc cgcatcagca agaccacggt aaaaaccatc ccggcgctga agtgaattaa
    14881 cctgatgtag cccatataga acagatacgt cgcctcgccg ctgacggaag gtagcggctt
    14941 gccgataaag tatccggtga ccatcaacac cgccatgcat aacaccgtca gccagtgcca
    15001 gatgcggact ggcgcttcaa agacatagtg gctgacaacg ttgtcgcttt tctgttgcat
    15061 gatgattctc cttcgctgtt aacgcacctg cacggagatc agctcgctac cgtcgtcgcc
    15121 cagcacgtgt gttgaacagg cgaggcacgg gtcaaagctg tgcagagtac gcaggatctc
    15181 cagcggttgc tcggggatcg ccattttggt gttcatcagc gccgcttcat aagcgccaat
    15241 ctgccctttg ggatcgcgcg ggctggtgtt ccaggtggtc ggcaccacgc actggtagag
    15301 atcaatcttg ccatcgcgaa tggcggccca gtggcctaac gccccgcgcg gcgcttcggt
    15361 aaagccgacg ccacggcact ctgtcggcca ggttgcaggt tcccattttt ccgtggaggc
    15421 ggtggcaaga ttgccgtttt tcaggttagt catcagcttg ttgaagaaat attgcaactt
    15481 acctgcggcc cactgtgctt cgtgcgcgcg gcacaaaatg cggcccaacg ttgactggat
    15541 accggaaagc ggcaggttca gcgccgacat catgcgatcg accgactcaa cggtcgcagc
    15601 atcgcctttg tgataagcga ttaacgtgcg tgccagcggc cccacttcca tcgcgttacc
    15661 gcgccagcgc ggcgctttga tccacgagta gcgttcctgt tcattcagct gctgaatgtt
    15721 ggtatcgctg cctttgacat cgccggggtt gtaccacggg tcggtaatgc catcgaatgg
    15781 atgacgcccg acctgatcgt tgggataacg ataccaggcg tggtcgacaa actcctgcac
    15841 ctgctgcgga tcaaccaaat ccactggcag cacattgttg aagtcgccgt taatcaccgc
    15901 gccgccaggc atcagcagac ttttctcacc aaagtcgttg gcaatatccg ggaacgcacc
    15961 gtagctgagg acacatttat cagaaagacc cgtgccgatt tcgctccacg gtttgttgaa
    16021 ctgaccgatg gctaaggcgt cggggatcat cacgttgtta atgaagtccg ccgtgcgggt
    16081 gataatcgac tgcaccaggt tcaggcgttc catattgacc gccccgaccg cgccgctttc
    16141 gtcaatgtta atggcgcaag gcatcccgcc gacaatccag tttggatgcg ggtttttacc
    16201 gccaaagacc gcgtggattt tgacaatttc acgctggaaa tcgagagctt cgagatagtg
    16261 ggcaaagccc atcaggttag cttctggcgg cagtttgtac tgcgggtgcc cccagtagcc
    16321 attgcggaag atccccaact gcccgccttc aacaaatttt ttcaggcggt tttgtacgtc
    16381 gaagaaatag ccaggggatg atttcggcca agaggagaga ctttgcgcca gttcggaggt
    16441 tttccgcggg tcggctttca gcgcatctaa cacatcgatc cagtccatcc cggcaagctg
    16501 atagaagtgc accagatgat cgtggcacca gagcgttgcc agcataatgt tgcggatgat
    16561 attggcgttg tccggcactt taataccgat agcatcttcg atggcgtaaa ccgaagccag
    16621 ggcgtgtacg ccagtacaga cgccgcagat acgttcaacg aacgcccacg catcgcgcgg
    16681 gtcgcgccct tgtaggatga tctccagccc gcgaaacatg gtgccgcagg agacggcatt
    16741 ggtgatcaca ttctgatcgt taatattcac ttcgcagcgc atgtggcctt cgatgcgcgt
    16801 aatcgggtcg accaccaggc ggcgtccggc attattgatg gtgtatccct gagtttcgta
    16861 ctgagtgctc atgcctgttt atcctcattg cctggctgat gttcggtttc tgtaggttgc
    16921 tggttatgac gtctgcgctg gtcaacggcg ctggcgactg cgtgcacacc aacagccgct
    16981 gccaccacac caagcgcggt taaaccgacg gtatcggcgg tggaatgagt acccatttgc
    17041 ggaatatcga ccacgcggct gtagaacgaa ccgcaatccc agaaaccatt ttccgcacag
    17101 cccaggcagc cgtgaccaga ctggattggg aaagaaacgc catcattcca gcgtgtggag
    17161 gaacaggcgt tataggtggt aggccctttg cagcccattt tgtacaggca gtaacctttg
    17221 cgggcagcgt catcatccca actctggacg aactctccgg cgtcgaagtg ggcgcggcga
    17281 tagcatttat cgtggattcg ctgaccatag aacatcagcg gacggcccat tctgtcgaca
    17341 tctggcaagc gatcaaaggt caccatgtaa gtaatgatgg cgctcatcac atccgggatc
    17401 ggcgggcagc caggtacttt gataatgggt ttgtcggtga tgactttgtc gataggcgtt
    17461 gcctgcgtcg gattgggcca cgcggcctgc acgcagcccc aggacgcgca ggttccccag
    17521 gcgataatcg cgctggctcc ggcagcggca cgtttgagtt tctcaataaa cggtcgaccg
    17581 ctgctgatac agaacatccc ctgctcgccc agcggcggat taccttctac tgcgaggata
    17641 tatttgccat tgtattgcgt gatgatgtct tcgaagactt cttccgcctg ggttccggcg
    17701 gcagccatca aagtatcgtc ataatcgagg gaaatcaggg aaaggatgac gtccttcgcc
    17761 agtgggtgag cggagcggat aaaagattcg gtacagcagg tgcattccag accgtggatc
    17821 cataccaccg gaatgcgggg tttgttctcc agcgcccagg caatctttgg tgccattccc
    17881 gcgcctaatc ccagcgacgt ggcagccaga ctacaatatt tgagaaagct gcgccgggta
    17941 acgccctgac gccgcatggc ctggtaaaat gtttcctcgt tattcatatc gcacgtctct
    18001 tctccttgcg acaccggcag gacaccgggc gcttatggtc ggcaagcgac gtccctgtag
    18061 caaggcaaac catcgcttca cgtgagatgc tgaaaacgaa agctcatcct tctgcactgg
    18121 cgcacgtcgc cagaaagtat tgttaataaa gcgtagtgaa a
//
LOCUS       DAJVRF010000078        18109 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJVRF010000078 DAJVRF010000000
VERSION     DAJVRF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18109
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.078"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..989
                     /locus_tag="OQN13_002454"
     CDS             <1..989
                     /locus_tag="OQN13_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1615788.1"
                     /translation="KEEFTLGKISRRDAVTQAIEANHYIYKNYSPAALIEKLKGFDSF
                     TMADWIERYKTILIRRKVSRNTYKIRANQLETIKEKLGEILLTEITTRHIAEFLDLWI
                     EGGKNTMAGSMRSVLSDMFREAIVEGRISQNPVTPTRAPKIVVTRERLKLKIYNCIRE
                     AADQLPAWFPLAMDLALVTGQRREDITNMRFSDIYDDRLHVRQIKTGMMIAIPLSLSL
                     PVAGLRLGTVVERCRLVSRGDYLISAGIRKNSPDGSIHPDGLTKKFVAARKFTGIQFS
                     ENPPTFHEIRSLAGRLYKETCGEEFAQRLLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1382..1723)
                     /locus_tag="OQN13_002455"
     CDS             complement(1382..1723)
                     /locus_tag="OQN13_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCS1615789.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1736..2608)
                     /gene="copD"
                     /locus_tag="OQN13_002456"
     CDS             complement(1736..2608)
                     /gene="copD"
                     /locus_tag="OQN13_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HCS1615790.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2612..2986)
                     /gene="yobA"
                     /locus_tag="OQN13_002457"
     CDS             complement(2612..2986)
                     /gene="yobA"
                     /locus_tag="OQN13_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCS1615791.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3125..3355
                     /gene="holE"
                     /locus_tag="OQN13_002458"
     CDS             3125..3355
                     /gene="holE"
                     /locus_tag="OQN13_002458"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCS1615792.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3457..4113
                     /locus_tag="OQN13_002459"
     CDS             3457..4113
                     /locus_tag="OQN13_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCS1615793.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4137..4799
                     /gene="exoX"
                     /locus_tag="OQN13_002460"
     CDS             4137..4799
                     /gene="exoX"
                     /locus_tag="OQN13_002460"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCS1615794.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4796..6856)
                     /gene="ptrB"
                     /locus_tag="OQN13_002461"
     CDS             complement(4796..6856)
                     /gene="ptrB"
                     /locus_tag="OQN13_002461"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCS1615795.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7065..7724)
                     /locus_tag="OQN13_002462"
     CDS             complement(7065..7724)
                     /locus_tag="OQN13_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCS1615796.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8051..8407)
                     /locus_tag="OQN13_002463"
     CDS             complement(8051..8407)
                     /locus_tag="OQN13_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCS1615797.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8474..8764)
                     /gene="yebG"
                     /locus_tag="OQN13_002464"
     CDS             complement(8474..8764)
                     /gene="yebG"
                     /locus_tag="OQN13_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HCS1615798.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            8898..10076
                     /gene="purT"
                     /locus_tag="OQN13_002465"
     CDS             8898..10076
                     /gene="purT"
                     /locus_tag="OQN13_002465"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCS1615799.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10132..10773)
                     /gene="kdgA"
                     /locus_tag="OQN13_002466"
                     /gene_synonym="eda"
     CDS             complement(10132..10773)
                     /gene="kdgA"
                     /locus_tag="OQN13_002466"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCS1615800.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10810..12621)
                     /gene="edd"
                     /locus_tag="OQN13_002467"
     CDS             complement(10810..12621)
                     /gene="edd"
                     /locus_tag="OQN13_002467"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCS1615801.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12856..14331)
                     /gene="zwf"
                     /locus_tag="OQN13_002468"
     CDS             complement(12856..14331)
                     /gene="zwf"
                     /locus_tag="OQN13_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCS1615802.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14669..15538
                     /gene="hexR"
                     /locus_tag="OQN13_002469"
                     /gene_synonym="yebK"
     CDS             14669..15538
                     /gene="hexR"
                     /locus_tag="OQN13_002469"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCS1615803.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15666..17108
                     /gene="pyk"
                     /locus_tag="OQN13_002470"
     CDS             15666..17108
                     /gene="pyk"
                     /locus_tag="OQN13_002470"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCS1615804.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17168..18010)
                     /locus_tag="OQN13_002471"
     CDS             complement(17168..18010)
                     /locus_tag="OQN13_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCS1615805.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4628 a         4554 c         4371 g         4556 t
ORIGIN      
        1 cgaaagaaga atttacacta ggtaaaattt caagaagaga tgcagtaacg caggcaattg
       61 aagcaaatca ttatatatac aaaaactact ctcccgctgc cttaattgaa aaacttaaag
      121 ggttcgactc atttactatg gcagactgga ttgaacgtta caaaacgatt cttataagga
      181 gaaaagtgtc cagaaatact tataaaattc gggcaaatca actggagaca ataaaagaaa
      241 aattgggaga gattttactg acagaaataa ccactcgcca tattgccgag tttcttgatt
      301 tgtggattga aggagggaaa aacacaatgg caggatcaat gcgttctgtg ttgtctgata
      361 tgtttcgcga ggccattgtt gaaggacgta tatctcaaaa tccagtcacg ccaacaagag
      421 caccgaaaat agtagttaca agagaacgac tgaaactaaa gatatacaac tgcatcaggg
      481 aggcagcaga tcaacttccg gcatggttcc cattagctat ggatttagcc cttgtaacag
      541 gacaacgtcg cgaagacata acgaatatgc ggttcagtga tatttatgat gatcgtctcc
      601 acgtcaggca aattaagaca ggaatgatga ttgctatccc cctgtcactc agccttcctg
      661 tcgctggtct acggcttggt acagtagttg aacgatgccg cctggtaagc cggggagatt
      721 atctaatcag tgccgggatt agaaaaaaca gccctgacgg cagcattcac ccggatggcc
      781 tgacaaaaaa atttgtcgca gccagaaaat tcacaggtat ccagttcagt gaaaacccac
      841 caacttttca cgagatcaga agcctggctg gacgattgta caaagaaaca tgtggagaag
      901 aatttgctca gcgtctactt ggccacacat cggagaagac aacaaaaatg tatcttgatg
      961 agagagaaaa aacgtactta ctgctctgat tttaacgtaa atggattgtt aaatgtattt
     1021 tggttgtgat ataaccaaaa agaccggaat acagaaattc gagtaaattt cggggaattt
     1081 cgggggagac atttgcaact aattgatttc aaatgcaatt aaaaaaagac cgaatacgat
     1141 tcctgtattc ggtccaggga aatggctctt gggagagagc cgtgcgctaa aagttggcat
     1201 taatgcaggc ttagttgcct tgccctttaa gaatagatga cgacgccagg ttttccagtt
     1261 tgcgtgcaaa atggtcaata aaaagcgcgg tggtcatcag cttaaatgtt aaaaaccgcc
     1321 cgttctggtg aaagaactga ggcggttttt ttattggaaa tcaaaaggct attttaggta
     1381 attaacagag tttttcagct cgttctataa acggtgccag actcattttt tcgccgggat
     1441 tgttaggatc atcaatctga atcaccgaaa tgggttgggc attggtcttc ccactggcaa
     1501 cttccttttg tgcgatatcg tttaaaggat actgcacgag ggtactcgga ttaataacat
     1561 acaaagcatt acccggtcgg caagtcagca tcacctcttc gcgattaaac gcccatttgt
     1621 ctttacccac ttcaaaacga ctgacggtaa tcacctgcgg tgcagccagc gccgctgcag
     1681 aactggtgag taacagaaac gccagaatac tttttttcat cattttcgta atccatcaaa
     1741 aaggttcgag agtcgcaagc aggctgatga tcaccagcac taccgcacct atcgcccatt
     1801 cgagtttagt catccacaca aaccagggtg ccgcacgatc ttcctcctgc ctcatgcgcg
     1861 gtacaagaac ataacgatta gccagcgcaa tgaccaccat aatcatcacc aggatggctt
     1921 tgagcaataa gagttggccc cagtaagtcg taagcgtggg gggaaatccg gtaatcaaca
     1981 atgcattaag cacgccgctt gccagtacgc cgatcaccgc aaaatgcccg caccatgaaa
     2041 aacacatcag cgcctgaata gcctggtgtc gccaacggcc tttgatgagt tgcatacacc
     2101 agagcaccgg cagtaatccc ccaaaccacg ccgccgcaca aatcaggtga atcgcgtgat
     2161 tagtctgatg gattttcgcc gttacccctt cattcagtgt cgcatgcccc attcccgcca
     2221 gcagaataaa ttgcgcggta gtgagcataa acagcaaacg tggcatattc cgcggttgca
     2281 taagggcgac gatcaacgtt acgagggcga gaacaatttg ccataaccag acgccaccaa
     2341 actgcgtttg caaaacagcc tgccaaatgt ttggcgaaaa tacatctgtc catcccgttc
     2401 ccatcaaccc gccctgaacg gcgagcattg cagtggcact gatcaaactc cagacggcag
     2461 cgtgctgttg taaacgcaga aaacgcttcg ccagcaagcg acgaatcgtc aagggggcca
     2521 gccaggcacc gtacattgcg aagccaaaaa ccagcatcag cgaggtgaaa tggataaatc
     2581 gcagcgcgat ccaggtaaac gccagcatga tttatttcac gctaaaggtg tagtgtcctt
     2641 tcgttttatg cccgtccacc gaaacaacat gccagtcaac ggtgtaggtt ccgggtttca
     2701 gcgaatcggc cagtggaaca atcagttgct tttggtcctg ctcatttcgc ttcgccggta
     2761 atgttttaat attttcgttt tttggccccg tgatttttgc accactgaat cctgtttcca
     2821 caccttccga gaagtttaaa gtgattgcct gcggtgcagc tgtcacttgc gcgtttgccg
     2881 caggatactg atgcgttaaa tgcgcatgtg cccagacgaa aggtgtcacc aacgaagtgg
     2941 tcaggattgc cagcgcgtag cgaagggagc gtgcagttga agccatatta tctattcctt
     3001 tttgtaataa cttttttaca gagcataacc ttgtctaatg tctgagtcga ggatcatcaa
     3061 ttccggcttg ccatcctggc tcactcttag taacttttgc ccgcgaatga tgaggagatt
     3121 aagaatgctg aagaatctgg ctaaactgga tcaaacagaa atggataaag tgaatgtcga
     3181 tttggcggcg gccggggtgg catttaaaga acgctacaat atgccggtga tcgctgaagc
     3241 ggttgaacgt gaacagcctg aacatttgcg cagctggttt cgcgagcggc ttattgccca
     3301 ccgtttggct tcggtcaatc tgtcacgttt accttacgag cccaaactta aataaaactt
     3361 atacagggtt acactttctt acataacgcc tgctaaatta tgagtatttt ctaaaccgca
     3421 cccataattt gcagtcattt tgaaaaggaa gtcattatgt cgttctggaa agttgcagca
     3481 gcgcaatatg agcctcgtaa aacctcactc accgaacaag tagctcatca tctggagttt
     3541 gtgagggccg ccgccaggca acaatgtcag ctgttagttt ttccatcgct ttctttactg
     3601 gggtgtgatt attcgcgacg tgcccttcct gccccacccg atctctcact gttagacccg
     3661 ctttgttatg ccgcaacgac ctggcgaatg accatcattg ccggccttcc cgttgaatat
     3721 aacgatcgct ttattcgtgg aattgcggtg ttcgcaccgt ggcgaaaaac gcctgggatc
     3781 taccatcaga gtcatggtgc atgtctgggc cgtcgttcca gaaccatcac ggtggtcgat
     3841 gaacaaccgg aaggtatgga tatggacccg acctgttcac tgtttacgac agggcaatgt
     3901 ctgggagaac ccgacctgct ggcgtcggcc cgtcgcttac aatttttttc acatcagtac
     3961 tcgattgctg tgctgatggc aaatgcccgt ggtaacagtg cactatggga tgaatatggt
     4021 cgtctcatcg ttcgcgccga tcgtggttca ttattgttag tcggtcagcg ttcatcacag
     4081 ggttggcaag gcgatatcat tccattacgc taggcttttt cggcctggag tatgccatgt
     4141 tgcgcattat cgatacagaa acctgcggtt tgcagggagg gatcgttgag attgcctctg
     4201 ttgatgtcat tgacggaaaa atcgtcaacc ccatgagcca cctggtgcgc cccgatcgtc
     4261 ctattagtcc acaagcgatg gcgattcatc gcatcaccga agccatggtc gccgataaac
     4321 cgtggattga agatgtgatc ccacactatt acggcagtga atggtatgtc gcgcacaacg
     4381 ccagctttga ccgccgcgta ctgcctgaga tgcccggtga gtggatttgc accatgaaac
     4441 tggcccgtcg cttgtggcca ggaatcaagt acagcaatat ggcgttatat aaaacacgga
     4501 agctcaatgt acagacgccg ccgggcctgc atcatcaccg cgcgttgtat gactgttata
     4561 tcaccgccgc gttgcttatc gatattatga acacctccgg ctggacggca gaacagatgg
     4621 ccgatatcac cggacgtccg tcgttgatga cgaccttcac ctttggcaaa taccgtggca
     4681 aagcggtttc cgacgttgcc gaacgcgatc cgggctatct gcgctggtta tttaataacc
     4741 tggacagcat gagcccggag ctgcgtttaa cactgaaaca ttatctggaa aatacttaat
     4801 ccgcaggcgt agcgggtaat gttccctgcg ccagcgcgac cagaaaagca tattccatcg
     4861 ctacgccttc gtacgattta aagcggccag atttaccgcc atggcctgag tccatgtcgg
     4921 tacagagcaa taaaagatgg ttatcggttt tcagctcgcg caatttagcg acccattttg
     4981 ccggttccca atattgcacc tgagaatcgt gtaaaccggt cgttaccagt aaatgcggat
     5041 aagcctgtgc ggtgacgttg tcatacgggc tgtagctttt catgtactcg taatattgcg
     5101 gatcctgcgg gttaccccac tcttcaaact caccagtggt aagaggaatt gattcatcaa
     5161 gcatcgttgt tacaacatca acaaacggta cctgggcgat aacgccgtgg aataattccg
     5221 ggcgttgatt aattgcaacg cccatcaaca tgccccccgc actcccgccc atcgcataac
     5281 aaagcgaagg agagccatag cccagtttta acaatgcatc gcaggcatca agataatcat
     5341 taaacgtatt tttcttcttc agaaattttc cgtcttcgta ccattgttgc cccagctcac
     5401 caccgccgcg aacatggaca atggcgtaga caaagccacg atctaacaaa ctcaagcggc
     5461 taaaactgaa atcggcatca atacttgcgc cgtaagaacc atagccatac accagcaacg
     5521 ggttgtgtcc tttgcgaaaa tgtttgcgat ggtagaccaa cgaaaccgga acttcgacgc
     5581 catcacgggc gactatccac aggtgttcac tgcggtaatt cgccgcatca aaaccaggaa
     5641 cttccgtttg ttttaataca cgacgctcat cggtatccat atccagttca aacaaagtgt
     5701 ctggtgtggt catggaagaa taaccataac gcaatcgcgc ggtttcaggt tctggattgt
     5761 aggcaatcca ggtcacatag gctggatcat caaaggcaat accaatgact tcccgggtct
     5821 tgcggttaat ttggcgcaaa ctggttaacc cgcgctgacg ctcttcaacc accagccagt
     5881 cggtaaacag cgtaaaccct tccagcatga tgttttcgca tggcggaatt aactcttccc
     5941 actgttgctc atcacgcata cgggtacggt ataagccaaa gtttttgccg tggcggttgg
     6001 aacgcagata aaaacgatgc tggtagtgat caaggctgta ttcgtgatct ttgcggcgcg
     6061 gcagaaaaac aaacggctcg gcatcggcca tttccgcgtc cagcaggcga acttcactgg
     6121 tggtggcgct ggccaaatga atgactacat agtgcttcga ggtcgtttta tgcaggctga
     6181 cgtaataggt atcgtctttt tcttcgtaga tcagtttatc ttgcgatgct ggcgtaccga
     6241 tggcgtgacg ccagacctga taaggcagca gcgtcaccgg atgcttgcga acatagtaga
     6301 aagtccagga gtcatttgcc cagacaaagc tgggttcaac gttatccagc agttccgggt
     6361 accagttacc agtttccaga ttacgaaaac gaatgccgta ctggcgtcgg gaaagaaaat
     6421 cttctgccag cgccataatg gtgttatcgg gcgtaatcgc cattccgccc atcgaataaa
     6481 actcactatg agctgcgcgc ttattggcat cgagcaatat ttcccactca tcccactctt
     6541 cactgaatgc cgattgacgc tggtagatag catattcaca gcctggttca taaatatgcc
     6601 gatagcggta gccatttttg atgtagggcg cagaaacttc tcgttgcgga atgcggtcga
     6661 tgatttcctt taagatgcga tcctgcaagg cttgttgtga ggccatcacc cgatgaccgt
     6721 aactattttc ttgttgcagg tagtccagga cttctggctg agaacgcgta tcatcccgca
     6781 gccagtagta attatcgatg cgcgtatcgc catgaagcgt catggcgtgg ggaatgcggg
     6841 cggcttttgg tagcatgtta ttgttctttc tggttgaaac atcttataag ggtggcaaaa
     6901 ctcaccgggg atgcaagcga aacaggggag tcattgctta gatgatgaca ggtaatggca
     6961 cggatatcga atgttatgca gacagagaaa ctcagcctgt tcaaatggct gtgcggttct
     7021 ggatagcccg aaatagtcaa cttcaggcta tccagagagc ggaattattc cgccaaagtg
     7081 cgtttttgct gttcgagatc gcgttcaatg ccgtcacgaa catcctgggg gattttcagc
     7141 gcgtcaccca gtgcattcag gtaactgcgt tccataaaat ggtcaatatc aatagccgcg
     7201 caactcagga aatagatttc cagcgcctct tcttcattgc ggaccccggt agccaggcgt
     7261 tgtggatcca gcggttgttc gattgcctgc tcaatgagta cacgcccctg ctcttccacg
     7321 ccggcttcac gcaattgctg gtcgattgcc gcacgttctt tggcatcaat atgaccatca
     7381 cttttagcgg caaagaccag cgcaaggatc aaacgttctg tacgctcatc cagcggcgta
     7441 ctttgcgcgc caaactgcgg ttcgtcctga tgcgccgcgc gaattttatc tttgtattta
     7501 ttccacagca ccgtacccgc taccgctccg ccgccaacca gtaacgcgtt ggtgccatat
     7561 ttcgtaagaa gtttacgtgc tgatttattt gcgaccagca gcccagccag tccgcctaat
     7621 gcgcctggca ctaacagttt gcccaatccc tgatccgcag acgaggaggt agaagaactg
     7681 ctttgcccaa gcagggattg cagttgattt aaccagttag ccatatttgc tcctcaataa
     7741 ccatttttag tgcctgtagc atagcggatg aggatgtcag aaaatgtata tcgagacaaa
     7801 agatgcgcaa ataagcgggg cggtaggccg ttattcaaag aaattcgcgc caggtaaggt
     7861 atttactgta tatcaggtga taagggtatt ttaggtgaaa gtagtttgtc gttttgagtg
     7921 gcattgtttg atgctgattg cccgatgcga cgctgttgcg tcttctcagg cctacgattg
     7981 tggtgcgttc tggggcggac aaggcactcg cgccgcatcc accaacggtc aatcgaatta
     8041 tcagaagcca ttaacgccgc tgatattccg ccattcccgc tttgcagtcc acgctgacct
     8101 ggtaatgaat atcggcactt ttaccacgca cggttagcgg tactgaccat ttatcatctt
     8161 taccctgaat gtcctgcaaa ctgacccaag ccacgggatc ggcctgaccg acaatttttt
     8221 gatcatctgc ccaacgcgcc acgcgatttt gttgataatc acgttttacg ctcgcggcaa
     8281 ttccggcagc atccagacct tcacactttg ggaaagtgac cgacttgctg gtttcattat
     8341 tggcagcgaa aactgatgcg caggcagaaa ccaacaacag ccctaaaaac gcccctcttt
     8401 ttttcatgtt tttctccata gcacaatgat tcaggagaaa gcatggtaca aattgtcagg
     8461 agcgcaagtt gcttcaggcc gcgtgtgagg catcttcctc ttcggattca gcaccgacga
     8521 cctgtgggga cggtaatttg ccggttttca gaatggtgct cagcacatct ttttgttctg
     8581 ccagccatag cgaaagggct tcacgttgct cgtcttccat ttgcactgga gaatttgtca
     8641 gccaggtgtc aagcagatcc gccgtatcaa gcattttgtc ataagcatcg gcttcctttt
     8701 tgctggtaaa cgacattttc tcttcgccct cacgaatgac tacgtattta acttcaaccg
     8761 ccatttgcag cctctcataa taactgtgat tttatacagt atatttcttt tcggttgaga
     8821 aatcaacatc agcaataaag acacacgcaa acgttttcgt ttatactgcg cgcggaatta
     8881 atcaggggat attcgttatg acgttattag gcactgcgct gcgtccggca gcaactcgcg
     8941 tgatgttatt aggctccggt gaactgggta aagaagtggc aatcgagtgt cagcgtctcg
     9001 gcgtagaggt gattgccgtc gatcgctatg ccgacgcacc agccatgcat gtcgcgcatc
     9061 gctcccatgt cattaatatg cttgatggtg atgcattacg ccgtgtggtt gaactggaaa
     9121 aaccacatta tatcgtgccg gagatcgaag ctattgccac cgatatgctg atccaacttg
     9181 aagaggaagg actgaatgtt gtcccctgcg ctcgcgcaac gaaattaacg atgaatcgcg
     9241 agggtatccg tcgcctggcg gcagaagagc tgcagctgcc cacttccact tatcgttttg
     9301 ccgatagcga aagccttttc cgcgaggcgg ttgctgacat tggctatccc tgcattgtaa
     9361 aaccggtgat gagctcttcc ggcaaggggc agacgtttat tcgttctgca gagcaacttg
     9421 ctcaggcatg gaagtacgct cagcaaggcg gtcgcgccgg agcaggccgc gtaattgttg
     9481 aaggcgtcgt taagtttgac ttcgaaatta ccttgctaac cgtcagcgcg gtggatggcg
     9541 tccatttctg tgcaccggta ggtcatcgcc aggaagatgg cgactaccgt gaatcctggc
     9601 aaccacagca aatgagcccg cttgcccttg aacgtgcgca ggagattgcc cgtaaagtgg
     9661 tgctggcact gggcggttat ggtctgtttg gtgtcgagct gtttgtctgt ggtgatgagg
     9721 tgattttcag tgaggtctcc cctcgtccac atgataccgg gatggtgacg ttaatttctc
     9781 aagatctttc agagtttgcc ctgcatgtac gtgccttcct cggacttccg gttggcggga
     9841 tccgtcagta tggtcctgca gcttctgccg ttattctgcc acaactgacc agtcagaatg
     9901 tcacgtttga taatgtgcag aatgccgtag gcgcagattt gcagattcgt ttatttggta
     9961 agccggaaat tgatggcagc cgtcgtctgg gtgttgcgct ggcgaccgct gagagtgttg
    10021 ttgacgccat tgaacgcgcg aagcacgccg ccggacaggt aaaagtacag ggttaaaccc
    10081 ggcaaaaaaa cgctacaaaa atgcccgatc gtagatcggg cgttttgact tttacagctt
    10141 agcgccttct acagcttcac gcgccagctt agtaatgcgg tcgtaatcgc ccgcttccag
    10201 cgcatctgcc ggaaccagcc aggaaccacc gatgcacagc acgcttttca gcgccaggta
    10261 gtcacggtag ttagccggag aaataccacc cgtcgggcag aaacggacct gggagaacgg
    10321 acccgcgatc gcctgcaggg ctttcacgcc gccgttagct tcagccggga agaatttgaa
    10381 ctctttcaaa ccgtagtcca tacccagcat cagttcggaa acagtgctga tccccggaat
    10441 tagaggaata gtcccttcgg tagcagcttt cagcagcggc tcggtcagac ccgggctaat
    10501 tgcgaactgt gcacccgctt cagtgacttc tgccagctgc tgtggattca gcaccgtacc
    10561 ggcacccaca atcgcttcag gcacttcttt ggcgatagca cggatagcgt caactgcaca
    10621 ctcggtacgc agagtcactt ccagaacgcg caccccacca gcaaccaacg cttttgccat
    10681 cggcactgcg tgttccagtt tttttaccac gataaccggt acaaccgggc cggtggtcag
    10741 gattgattct gcacttgttt tccagttttt catcagagtt ttctctcgcc tgattacaaa
    10801 tttgtcgtct taaaaagtga tacaggttgc gccctgttcg gcaccggaca gtttttcacg
    10861 caaggcgctg aataattcac gtcctgtccc cacgcgtgac gcgctcaggt caggaatgtg
    10921 cggttcgcga gcagccagtt ccgcttcgtc taccagcagc gtcagttcgc ctgtctgtcc
    10981 attcacacga atgatgtccc cgtcgcgcac ttttgccagc agcccgccat cgtaggcttc
    11041 tggtgttacg tggatagctg acggcacttt acctgaagcg ccggagagtc gtccatcggt
    11101 aactaacgca attttgaaac accggtccaa taatacacca agtggcggca tgagtttatg
    11161 taattctggc attccgttcg cttttggccc ctgatgacgg acaacaacga cacaatcgcg
    11221 gtccagcaaa cccgcttcaa aggccggcat aacgtcatgc tggctttcaa aaacaaccgc
    11281 tggcgcttca atcacctggt tctcaaccgg cacggcagag gttttcataa ccgcacggcc
    11341 caggttaccg cttaacactt ttgtcccacc atgatgagag aaaggttgtt cgaaggaagc
    11401 gatcacattg ctgtcgagtg atttttccgc cccttcccgc cagtcctgtt caccattatt
    11461 cagccatggt tcaagggtat aacgagacag accaaagcct gccaccgtat tgacatcttc
    11521 atgcagcagg cctgctttga gcagttcacg caccagaacc ggtacgccac ctgccgcctg
    11581 gaagtggtta atatcggccg gaccgttcgg gtagagacgt gccatcagcg gtacaacatc
    11641 agaaaggtca gagaagtcat cccagttaat ctgaataccg gccgcgcgcg ccatcgccac
    11701 caggtgcatg gtgtggttag tggaaccacc ggtcgccagc agtgcaacga taccgttcac
    11761 caccactttc tcatcgatca tcttaccgat cggcatccat tcattaccat taccggtcat
    11821 gcgtgtaacc tgacgcgcag cggcagcggt caaagcatcg cgcagcggag aatccggatg
    11881 aacaaaagaa gagcctggca actgcatccc cataaattcc accaccatct ggttggtgtt
    11941 ggcagtaccg tagaaagtac atgttcccgg cgcatggtaa gacgcggctt ctgactccag
    12001 taaggccatg cggtccactt taccttcggc ataaagctgg cgaatacgca ctttttcttt
    12061 atttggcaaa ccgcttgcca tcggtccaga cggcacaaac accgcaggca aatgaccaag
    12121 cgacagggct gccatcgtca gacccgggac aatcttgtcg cacacaccga ggaacagagc
    12181 accatcaaac atgttatggg acagccccac cgccgcagac atcgctatca cttcgcggct
    12241 tagcagcgac aattccattc catcctgccc ctgggtgaca ccatcacaca tcgccggaac
    12301 accgcccgca acctgaccaa ccgcattcgc ttcatgcagg gctttacgaa tgatttctgg
    12361 atagtgttca taaggctggt gcgcggagag catgtcgtta taggaggtga tgatggcgat
    12421 attgttacgc aacatgcttt tcaaagaggc tttgtcttct ggctggcagg cagcgaaacc
    12481 gtgtgccagg ttaccgcatg ccaactgcga acgatgaacg gtcgacgttt tcgcttgttc
    12541 tatccgggcg agataagcag agcgagtctc gcgcgaacgt tcaatgattc gatttgttac
    12601 gcataacaat tgtggattca taaaggctcc tgaaattgag ttgtcagagc aggatgattc
    12661 acaacgcgtt tcattcagag gatttatgag tgaaacgcct gtaaccggag ctcatagggc
    12721 aaaaacgttt cagtcagtgt aataaaaaaa gcctcgtggg tgaatccgca cgaggcctga
    12781 aagtgtaaaa attgttctac aatctgtgca agatcatgtt accggtaaaa taaccataaa
    12841 ggataagcgc agatattact caaactcatt ccaggaacga ccatcacggg taatcatcgc
    12901 caccgaggca acgggtcccc aggttccggc ctgatacggt ttcggcgcat cattgtccat
    12961 cgcccacgcc tcagtaatgg agtctaccca tttccaggct tcttccactt catcacgacg
    13021 tacaaacagt gcctgaatac cacgcatggt ttccagcagc agacgttcat aggcatccgc
    13081 cagatgcgtc tgattaaagg tttctgaata gctcagatcc agcttggtga tttgcaggtt
    13141 atgtttgtgg tcaaggccag gaactttatt cagtacctgg atatccacgc cttcatcagg
    13201 ttgcagacgg atagtcagtt tattctgcgg cagatcctgc cacgactctt taaacagatt
    13261 cagttcaggt gttttgaaat agaccacgac ttcagaacat ttggtcggca gacgtttacc
    13321 agtacgcagg tagaatggca caccggccca gcgccagtta tcaatgtcga cgcggatcgc
    13381 cacgaaggtt tctgtattgc tgctcttgtt cgcgccctct tcttccagat atcccggcac
    13441 ttttttgccc tgggcgaagc ccgcagtata ttgcccgcgt acggtttttt cgcgtacgtt
    13501 ggagcggtcg atgcggcgca gggacttcag tactttcact ttttcatcgc ggatgctgtc
    13561 tgcgctcagg tcagatggcg gagacatcgc aatcatgcag agaatttgta gcaggtggtt
    13621 ctgaatcatg tcacgcatct gaccggcttt atcaaaatag ccccagcgcc cttcgatccc
    13681 cacttcttct gccacggtaa tctcaacatg atcaatggtg cgattgtccc agttattcac
    13741 aaacagggag ttagcaaaac gcagcgccaa caggttcagc accgtttctt taccaagata
    13801 gtggtcgata cggtaaacct ggcactcctc gaagtattcg ccaacctgat cattgatttc
    13861 ctgcgaggtc gccagcgacg tccccagcgg tttctccatg actacgcgtg ccggtttagc
    13921 attcagtttt gcctcgccaa gccctttgca aattgcgcca aaagtgctgg gcggcatggc
    13981 aaagtagtta atggtgatac gatttttttg atccagcatc gcgccgagac ggttgaatgc
    14041 agcagtgtca ttgacatcga gattacaaaa atccagacgt gcactcaggg tgtcccataa
    14101 accttcatca atggtttctt tcatgaaagt ttcgagcgcc tcgcggacaa ctttggtata
    14161 tgccgcttta tcccagtcag cacgccctac gccgataatc cgggtgtccg ggttgagctg
    14221 accggctttt tccagttgat acagggaagg cagcaattta cgacgcgcaa ggtcgccttt
    14281 cgcgccgaaa atgaccaggt cacaggcctg ggctgtttgc gttaccgcca tgtcattctc
    14341 cttaagttaa ctaacccggt acttaagcca gggtatactt gtaattttct tacggtgcac
    14401 tgtactgctt ttacgagctt gcgaaaactg taaacgctta tccacccgtg cgattacggg
    14461 aaaagcgcgc aaagtgcggc aaaactgata aaaaaatcat cgttttccat tcggtaaaaa
    14521 tctgacactg atcatgttat gaaaaaaaat aacaactttt ttatctgctt ttgtcattaa
    14581 cacggcacac aggcgtaata tctgacaaaa ctgcatttcg atttctttca gtgcggaaat
    14641 cgtcattacc cgtgagtctc tttacatcat gaatatgctg gaaaaaatcc agtctcagct
    14701 ggaacatttg agcaaatcag agcgcaaagt tgccgaggtc attctggctt cgcccgataa
    14761 cgcgatccat tcgagtattg ctgctatggc actggaagcc aatgttagcg aaccgacggt
    14821 gaatcgtttc tgtcgcagca tggacacgcg cggttttcct gattttaaac ttcatctggc
    14881 acagagtctg gcgaatggca ctccctatgt taatcgcaat gtcaatgaag atgacagcgt
    14941 tgaatcatac acagggaaaa tatttgagtc cgcaatggca acgcttgatc atgtccgtca
    15001 ttcactggat aaatctgcca tcaaccgcgc cgtcgacttg ctcactcagg caaaaaaaat
    15061 cgcctttttc ggattaggct cttcagccgc cgttgcccac gatgcgatga ataagttctt
    15121 tcgttttaat gttccggtgg tgtactccga tgatatcgtg ctgcaacgca tgagttgtat
    15181 gaattgtagc gacggagacg tggtggtgct gatttctcac actggaagaa caaaaaatct
    15241 ggtcgagctg gcgcagctgg ctcgcgaaaa cgacgcaatg gtgattgccc tcacctctgc
    15301 gggtaccccg ctcgcccggg aagcaacgct ggcaattacc ctcgacgtac cggaagatac
    15361 tgacatttat atgcccatgg tttctcgact tgcacagctg accgtgatag atgtgctggc
    15421 gacaggattt actttgcgac gcggtgcaaa attcagagat aacttgaagc gggtcaagga
    15481 agcgctgaag gaatcgcgtt ttgataagca gttacttaat ttaagtgacg atcgctaaaa
    15541 acgactgtca ctgtcctaat cttatacgac atccgaatga gattaattta tcgccatcgc
    15601 ggcgttattt cattcggatt tcatgttcaa gcaacacctg gttgtttcag tcaacggagt
    15661 attacatgtc cagaaggctt cgcagaacaa aaatcgttac cacgttaggc ccagcaacag
    15721 atcgcgataa taatcttgaa aaagttatcg cggcgggtgc caacgttgta cgtatgaact
    15781 tttctcacgg ctcgcctgaa gatcacaaaa tgcgcgcgga taaagttcgt gagattgccg
    15841 caaaactggg gcgtcatgtg gctattctgg gtgacctcca ggggcccaaa atccgtgtat
    15901 ccacctttaa agaaggcaaa gttttcctca atattgggga taaattcctg ctcgacgcca
    15961 acctgggtaa aggtgaaggc gacaaagaaa aagtcggtat cgactacaaa ggcctgcctg
    16021 ctgacgtcgt gcctggtgac atcctgctgc tggacgatgg tcgcgtccag ttaaaagtac
    16081 tggaagttca gggcatgaaa gtgttcaccg aagtcaccgt cggtggtccc ctctccaaca
    16141 ataaaggtat caacaaactt ggcggcggtt tgtcggctga agcgctgacc gaaaaagaca
    16201 aagcagacat taagactgcg gcgttgattg gcgtagatta cctggctgtc tccttcccac
    16261 gctgtggcga agatctgaac tatgcccgtc gcctggcacg cgatgcagga tgtgatgcga
    16321 aaattgttgc caaggttgaa cgtgcggaag ccgtttgcag ccaggatgca atggatgaca
    16381 tcatcctcgc ctctgacgtg gtaatggttg cacgtggcga cctcggtgtg gaaattggcg
    16441 acccggaact ggtcggcatt cagaaagcgt tgatccgtcg tgcgcgtcag ctaaaccgag
    16501 cggtaatcac ggcgacccag atgatggagt caatgattac taacccgatg ccgacgcgtg
    16561 cagaagtcat ggacgtagca aacgccgttc tggatggtac tgacgctgtg atgctgtctg
    16621 cagaaactgc cgcagggcag tatccgtcag aaactgttgc agccatggcg cgcgtttgcc
    16681 tgggtgcgga aaaaatcccg agcatcaacg tttctaaaca ccgtctggac gttcagttcg
    16741 acaatgtgga agaagctatt gccatgtcag caatgtacgc agctaaccac ctgaaaggcg
    16801 ttacggcgat tattaccatg accgaatcgg gtcgtaccgc gctgatgacc tcccgtatca
    16861 gctctggtct accaattttc gccatgtcgc gccatgaacg tacgctgaac ctgactgctc
    16921 tctatcgtgg cgttacgccg gtgcactttg atagcgctaa tgacggcgta gcagctgcca
    16981 gcgaagcggt taatctgctg cgcgataaag gttacttgat gtctggtgac ctggtgattg
    17041 tcacccaggg cgacgtgatg agtaccgtgg gttctactaa taccacgcgt attttaacgg
    17101 tagagtaatc tcacctgccc cggccgctga tggccggggt atttagcaac acataaatta
    17161 ggactactta ttcaatccaa tcataatcac agccccctgt aaaatgaata cagggatagc
    17221 ctgaatggag atctaaatag cttgcaagac gtcgcatacc cgcttcctct gcaacactat
    17281 gtccaataac tgccatcggt aagccactat ctttggagta ttgtgtctgc acccatgttt
    17341 taattgaatc gttaatcgta atataataat cagcctgata ttccatatat tgtattggat
    17401 cacaaaaaca gcctgcccca atgccaacag ttctgactac tctggcttta tctccataaa
    17461 attgaatgcc aggaagatta tatattttca aatttttggc aaaacgtttg gtaatgttaa
    17521 tggcactgtc agggtctata gcatatacac agtacatctc atcagaagca acaatatttg
    17581 taacatccag ttctaactgc tgagccaaag ccttagacat tccgaacgta ggctctcgat
    17641 ccatgacatc atggcaacga atgattacca aattattttt atgcaaaaac tccttttttt
    17701 gctcaattat ttttaaataa ctgagttcac cgcgagtata atccattatt tttctggccc
    17761 atgaaaactc aagatcgtca ccctccagat cagcataaga ataaaatgta ggctcatgtg
    17821 taataatgat attgacacct tcaaaaacag cctttcttaa aacatccatt gaggcaatcc
    17881 agcaggtccc aattttatta ataagtgtcg cctcagcacc aaaaataatt ttatcaacag
    17941 ttggttgcga aacagcaacc attgaattta aaaaacaatt aagatccgcg gctgttttaa
    18001 tatgatgcat tcaacatcct tcacaggttt catcagcaat aaaataattt caacatttct
    18061 gggaaacgtt ttgcccatgc agactcagag tgggtatcac ctccccata
//
LOCUS       DAJVRF010000079        17962 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJVRF010000079 DAJVRF010000000
VERSION     DAJVRF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17962
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.079"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="OQN13_002472"
     CDS             complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="OQN13_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCS1615806.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1598..4654
                     /locus_tag="OQN13_002473"
     CDS             1598..4654
                     /locus_tag="OQN13_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCS1615807.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4651..5061
                     /gene="menI"
                     /locus_tag="OQN13_002474"
     CDS             4651..5061
                     /gene="menI"
                     /locus_tag="OQN13_002474"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCS1615808.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5161..5349
                     /locus_tag="OQN13_002475"
     CDS             5161..5349
                     /locus_tag="OQN13_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCS1615809.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            5897..6265
                     /gene="sufA"
                     /locus_tag="OQN13_002476"
     CDS             5897..6265
                     /gene="sufA"
                     /locus_tag="OQN13_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCS1615810.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6274..7761
                     /gene="sufB"
                     /locus_tag="OQN13_002477"
     CDS             6274..7761
                     /gene="sufB"
                     /locus_tag="OQN13_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCS1615811.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7771..8517
                     /gene="sufC"
                     /locus_tag="OQN13_002478"
     CDS             7771..8517
                     /gene="sufC"
                     /locus_tag="OQN13_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCS1615812.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8492..9763
                     /gene="sufD"
                     /locus_tag="OQN13_002479"
     CDS             8492..9763
                     /gene="sufD"
                     /locus_tag="OQN13_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCS1615813.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            9760..10980
                     /gene="sufS"
                     /locus_tag="OQN13_002480"
     CDS             9760..10980
                     /gene="sufS"
                     /locus_tag="OQN13_002480"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCS1615814.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            10993..11409
                     /gene="sufE"
                     /locus_tag="OQN13_002481"
     CDS             10993..11409
                     /gene="sufE"
                     /locus_tag="OQN13_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCS1615815.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11558..12562
                     /gene="ldtE"
                     /locus_tag="OQN13_002482"
     CDS             11558..12562
                     /gene="ldtE"
                     /locus_tag="OQN13_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCS1615816.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="OQN13_002483"
     CDS             complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="OQN13_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCS1615817.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(13171..14583)
                     /gene="pykF"
                     /locus_tag="OQN13_002484"
     CDS             complement(13171..14583)
                     /gene="pykF"
                     /locus_tag="OQN13_002484"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCS1615818.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            15140..15349
                     /gene="fumD"
                     /locus_tag="OQN13_002485"
     CDS             15140..15349
                     /gene="fumD"
                     /locus_tag="OQN13_002485"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCS1615819.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            15805..16431
                     /locus_tag="OQN13_002486"
     CDS             15805..16431
                     /locus_tag="OQN13_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCS1615820.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            16452..>17962
                     /locus_tag="OQN13_002487"
     CDS             16452..>17962
                     /locus_tag="OQN13_002487"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCS1615821.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKIKDFYYRLAHRVGELSHLADGSYAIAER
                     WNLGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINF"
BASE COUNT         4588 a         4256 c         4724 g         4394 t
ORIGIN      
        1 agctgaatgg cggtaagcag gaaaatatgg ggaagagcaa gcaaatatca attagcggtg
       61 attaacaatc aggcggcaga gccgcctgat gagaaattac ttattcggtt tctcgatttc
      121 gccaagttct tcgagaattt cttccggttg gtccgtcggc ggcgggactt cttccaccca
      181 cgcggcaaac agacgccagg aaacggctaa cagaaccgga ccaataaaca gaccgatcat
      241 cccgaaagca atcaaaccac caataacgcc agagaggatc aggatcagcg gtaaatcggc
      301 acccatgcga attaacattg ggcggatgac gttatccagc gtgccaacca caccgctcca
      361 cactaacaat accgttcccc aggtggtatc gccggtccag tagagccaga taatcgccgg
      421 aatcagtacc ggcaacgggc caagctggac aaggcaggag aggatcatta acaccgttag
      481 caacgttgca taaggtacgc cggatacggc gaggccgata ctgccaagca ctgcctgtac
      541 taacgccgtc accaccacac ccagcgccac cgcgcggata gcctgtgccg ccagcagcac
      601 ggcggcatct ccgcgaacgc ctgccagacg ggttgcaaaa tggcgaatgc cttgtgccac
      661 ctgttcaccg cgccagtaca gcagggcact gaagagaagc atcagcgcac aatgcaccat
      721 aaagcgcccg atatgcgccg cctgcccaac gaaccaggtg gtggtggtgc caatataagg
      781 gcggactttc gccatgatcg ccgtaccccc catatccagc aagttgtgcc agcctgcata
      841 cagcttcgcg ccaatcaccg gaatggtatt aagccacgcc agatcgggta acgtcatgtc
      901 gccgctggaa atggctttaa ttagcgggcc gctgccgtcg acgatactgt taaccagcaa
      961 agcaataggg atgataaaca ccatcactaa taacagcgtc atcaccagaa cggcgagaga
     1021 gcggcggcca aacatgatct tttgcaaacg taacaatacc ggccaggtgg cgataaccac
     1081 cgtaccggcc catgcaaagc cgagaataaa gggttgaaca atccacagac atgccacaat
     1141 catgatggct aaaaacagca ccgaaagcag aatttgtgcg acatccctgg gctgacgaac
     1201 atttaccata actacttttc accttttttg tgcgtcaaat cgtcgacgcg acgtgaacac
     1261 gcgaaactca cctgcataat gatgagcaat ttcagcggtt tttaacaggc cgattctgcc
     1321 cttaattctg atgggcgtgt tagaaaaaaa tgtggtacaa caactcaggc aacacgcaaa
     1381 cgatttactc gtcgtatttc aagttgcatg cgctgcggct acgttcgttc accccagtca
     1441 cttactattg taagctcctg gagttcactc gcttgccgcc ttcctgcaac tcgaaatcca
     1501 tagagtaaat aaacatcacc tttcagtgcg ctattgcagc cgcctgaaag aatagtgtat
     1561 cgacctacat cacagaccgc aggaaagggt caatataatg attccacaga tttcccaggc
     1621 acccggcgtc gttcaactgg tgcttaattt tttgcaagag ctggagcaac aaggttttac
     1681 cggcgatacg gcgacaagtt atgccgatcg tctgacaatg tcgaccgaca acagtattta
     1741 ccaacttctc cccgatgcgg tggtatttcc gcgttcaacc gcagatgtgg cgctgatcgc
     1801 ccgtcttgcc gcgcaggaac gctattcatc gctgatcttt accccccgcg gcggcggcac
     1861 cggcactaac ggtcaggcgc tcaaccaggg gattattgtt gatatgtccc gccatatgaa
     1921 ccgcatcatc gaaattaacc ctgaagaggg ctgggtgcgc gttgaggccg gggtgataaa
     1981 agatcaactc aatcagtacc tgaaaccgtt cggctacttt tttgcaccag aactttcgac
     2041 cagcaaccgg gcaacgctcg gcgggatgat caataccgat gcatccggtc agggatcgct
     2101 ggtctatggc aaaacgtcag atcacgtact tggcgtacgc gcggtgttgt tggggggcga
     2161 tattctcgat acgcaacctt tacccgtcga actggcggaa acgctgggta aaaccaatac
     2221 cacaatcggg cgaatttata acacggttta tcaacgttgc cgtctgcaac gccagttaat
     2281 catcgacaac ttccccaaac ttaaccgctt tcttaccggt tacgatctgc gtcatgtctt
     2341 taacgatgag atgaccgagt tcgacctgac gcgcattctg acgggttcag aagggacgct
     2401 ggcctttatt accgaagcgc ggctggatat tacgcccttg cctaaagtgc gccgtctggt
     2461 gaacgtcaaa tatgactctt ttgactccgc gctgcgtaac gcgccgttta tggttgaggc
     2521 gcgggcgctt tcggtagaga cggtggactc aaaagtgctg aatctggcgc gggaagatat
     2581 tgtctggcat tccgtcagcg agttgattac cgatgtgcct gacaaagaga tgctcgggct
     2641 gaacattgtg gaatttgctg gtgatgatga ggcgctgatt gacgagcgag ttaatgcact
     2701 ctgtgtgcgg cttgatgagc tgattgccag ccaacaggca ggtgtgattg gctggcaggt
     2761 gtgccgcgag ctggcggggg ttgaacgtat ctatgcaatg cgcaaaaaag ccgttggtct
     2821 gcttggcaat gccaaaggtg ccgctaagcc aattccgttt gctgaggata cctgcgtacc
     2881 gccggaacat ctggccgatt atattgctga atttcgcgcg ctgctcgaca gccacggctt
     2941 aagctacggt atgttcggtc acgtcgacgc aggtgttttg cacgtccgtc cggcactgga
     3001 tatgtgcgat ccgcaacaag agattttgat gaagcaaatc tctgatgacg tggtggcgct
     3061 gactgcgaaa tacggtggtt tgttgtgggg cgagcacggc aaaggttttc gcgctgaata
     3121 cagcccggcg tttttcggtg aggaactttt tgcagaactg cgcaaagtga aagcggcatt
     3181 tgacccgcat aaccgactca acccagggaa gatttgcccg ccagaaggtc tcgatgcgcc
     3241 gatgatgaaa gtggacgcgg tgaagcgcgg tacattcgat cggcagatcc ccattgcggt
     3301 acgccagcag tggcgcggtg cgatggagtg taacggcaac ggtttatgct tcaactttga
     3361 tgcccgtagt ccgatgtgtc cgtcgatgaa gatcacccag aaccggattc attcaccgaa
     3421 agggcgcgca acgctggtgc gtgaatggct gcgtttgttg gcggatcgcg gcgttgatcc
     3481 actcaaactg gaacaagaac tgcctgaatc cggcgtcagt ttacggacgt taattgcccg
     3541 cacgcgcaat agctggcatg cgaataaagg cgaatatgac ttctcacacg aagtcaaaga
     3601 ggcgatgtcg ggctgtctgg cctgtaaagc gtgttcgacc cagtgcccca tcaaaattga
     3661 tgtgccggag tttcgctctc gtttcctgca gctctatcac acccgttatt tacgcccgct
     3721 gcgcgaccac ctcgtcgcta cggtcgagag ctacgcaccg ctgatggcac gcgcgccgaa
     3781 aacctttaac ttcttcatta accagccgct ggtgcgcaaa ctctcggaaa aacatatcgg
     3841 catggttgat ttgccactgc tgtcggtccc ctcgctacaa caacaaatgg tggggcatcg
     3901 ctcggcaaac atgacgctgg aacagcttga agctctcaat gcagagcaga aagcgcgcac
     3961 ggtgttggtg gtgcaggacc cctttaccag ctattacgat gcgcaagtgg tggcggattt
     4021 tgtccgtctg gttgaaaaat taggtttcca gcctgtgtta ctgccatttt cgccaaatgg
     4081 caaagcccag catatcaaag gctttcttaa tcgttttgcg aagacggcga aaaagacggc
     4141 ggatttcctc aaccgtatgg ctaagcttgg gatgccaatg gtgggcgtcg atccggcgct
     4201 ggtactttgt tatcgcgatg aatataaact ggcgctgggc gaggaacgtg gcgagtttaa
     4261 cgtcttactt gcgaatgaat ggctggcaag cgcacttgag tcacatccag tggctacagt
     4321 cagcggtgaa tcatggtatt tctttggtca ctgtaccgag gttaccgctt tgccgggcgc
     4381 gccagcacaa tgggccgcga tatttgcccg ttttggcgcg aaactggaaa atgtcagcgt
     4441 gggttgctgc ggcatggcag ggacttacgg acatgaagcg aaaaaccatg aaaattcgct
     4501 cgggatctat gagttatcct ggcatcaggc tatgcagcga ctgccgcgta accgctgtct
     4561 ggcgaccgga tattcctgcc gtagccaggt aaaacgggtt gaaggcacgg gggtacgcca
     4621 tcctgtgcag gctttactgg agattattaa atgatatgga aacggaaaat caccctggaa
     4681 gcactgaatg ctatgggtga aggaaacatg gtggggttcc tggatattcg ctttgaacat
     4741 attggtgatg acacccttga agcgacaatg ccagtagact cgcggacaaa gcagcctttc
     4801 gggttgctgc atggaggagc atccgtggta ctggccgaaa gtatcggttc cgttgccggt
     4861 tatttatgta ccgaaggtga gcaaaaagtg gttggtctgg aaatcaatgc taatcacgtc
     4921 cgctcggcac gagaagggcg ggtgcgcggc atatgtaaac cgttgcatct cggttcgcgt
     4981 caccaggtct ggcagattga aatcttcgat gagaaagggc gtttgtgctg ttcgtcacga
     5041 ttgacgaccg ccattttgtg acgttgtcac cagaaaagtg tgacgcatat tgcttttggt
     5101 cattatctgc acagtaaacg gtggtatact gtgcacgtct tgtctaaaag cgaggatgct
     5161 atgtctactc aacttgatcc cacccagctg gccattgaat ttttacgccg tgaccagagc
     5221 aatctttcac ctgcccaata tctcaaacgt ctgaaacaac tagaacttga atttgctgac
     5281 ctgcttactc tctcttctac tgaattaaaa gaagaaatct actttgcctg gcgtctgggc
     5341 gtgcattgat cctgacttaa tgccgcaagt tcgccaaatc gacctggatt aacctccgtc
     5401 caggttcgat gaccttctta atactgctta tctaccgcct gatattgttc aaaaataata
     5461 ctaatcgcag tttgtgttaa aacggcgggt tagctttatg agattattct tatcgcccct
     5521 tcaagagcta agccactgag agtgccggag ataagcgccg gatggggtag aaacccttaa
     5581 gcctgtgtcg cacagactta agggttttct tatttctgaa cgctgcactt ttttacctat
     5641 ctcttaaata gtcattttca atacaacttt ttctttttca tcacagcctt aatgctcctt
     5701 agataataac cattatctaa caatgagata cctaattctt agcgtgcctg ttaacccaca
     5761 aatcagggtc tatgcttatt aaaagcaggc aaaaagcatg ttttacggta aagcccctgc
     5821 gtttgctggg ttgaactgat aatcattatc actagcatgc tgttatacgc tgaaagcgat
     5881 gaagtgaggt aaatcgatgg acatgcattc aggaaccttt aacccacaag atttcgcctg
     5941 gcaaggctta acgctgacac ccgcagcggc gatacacatc cgtgagctgg tggcaaagca
     6001 gccgggtatg gtcggcgtgc gcttaggcgt gaagcaaacg ggctgcgcgg gctttggcta
     6061 tgtgctcgac agtgttagcg agccggacaa agacgatctg ctgtttgaac acgacggcgc
     6121 gaagctgttt gtcccgctgc aagcgatgcc gtttattgat ggcacggaag tcgatttcgt
     6181 tcgtgaagga cttaatcaga tattcaaatt tcacaaccct aaagcccaga atgaatgtgg
     6241 ctgtggcgaa agctttgggg tataggcggt actatgtctc gtaatactga agcaactgac
     6301 gatgtcaaaa cctggaccgg cggcccgctg aattataaag aaggattctt cacccagtta
     6361 gccaccgatg agctggcaaa ggggataaac gaagaggtgg tgcgcgcaat ttcggcgaag
     6421 cgtaatgagc cggagtggat gctggagttt cgtctaaacg cctatcgcgc atggctggag
     6481 atggaagaac cgcactggtt gaaagcgcac tacgacaagc tgaattatca ggattacagt
     6541 tactactcag caccatcgtg cggtaattgt gacgacactt gcgcgtctga acctggcgcg
     6601 gtgcagcaaa ctggcgcgaa cgccttttta agtaaagagg tggaggcggc gtttgagcag
     6661 ttgggcgttc ccgtgcggga aggcaaagag gtggcggtgg atgccatttt cgactctgtt
     6721 tcggttgcca ctacttatcg cgaaaaactg gcggagcagg gaattatttt ctgttccttt
     6781 ggtgaggcga tccacgatca tccggaactg gtgcgtaaat atctcggcac tgtggtgccg
     6841 ggaaatgaca acttctttgc cgcgcttaat gcggcggtag cctctgacgg tacgtttatt
     6901 tatgtgccta aaggcgtgcg ctgcccgatg gaactttcca cctattttcg cattaacgcg
     6961 gaaaaaaccg ggcagtttga gcgcaccatt ctggtggccg acgaagacag ctacgtcagc
     7021 tacattgaag gctgttccgc tccggtgcgt gacagctatc agttacacgc ggcggtggtg
     7081 gaagtcatca tccataaaaa cgccgaggtg aaatattcca cggtacaaaa ctggttccct
     7141 ggcgataaca acaccggcgg tattctcaat ttcgtcacca agcgtgcttt gtgcgaaggc
     7201 gaaaacagca aaatgtcatg gacgcaatca gaaaccgggt cagcgattac gtggaaatat
     7261 cccagctgca ttttgcgcgg cgataactcc attggtgagt tttactcagt ggcgctgacc
     7321 agcggtcatc agcaagcgga taccggcacc aagatgatcc acatcggtaa aaacaccaaa
     7381 tcgaccatta tctcgaaagg gatctctgcc ggacatagtc agaacagtta tcgcggctta
     7441 gtgaaaatca tgccgacggc aaccaatgcg cgcaatttca ctcagtgcga ctcaatgctg
     7501 attggcgcta attgtggggc gcataccttc ccgtatgtcg aatgtcgcaa taacagcgca
     7561 caactggagc acgaggcaac gacatcacgt attggtgaag atcaactgtt ttactgcctg
     7621 caacgcggga ttagcgaaga agacgccatc tcgatgattg ttaacggttt ctgcaaagac
     7681 gtgttctcag aactgccgct ggaatttgcc gttgaagcac aaaaactcct cgccatcagt
     7741 cttgaacaca gcgtcggata aggaataaac atgttaagta ttaaagattt acacgtcagc
     7801 gtggaagata aagctatcct gcgtggatta agcctcgacg ttcgtcccgg cgaagttcac
     7861 gccattatgg ggccaaacgg ttcgggcaaa agtaccttat cggcaacgct tgccgggcga
     7921 gaagattatg aagtgacggg cggcacggtt gagttcaaag gcaaagattt gcttgcgctg
     7981 tcgccggaag atcgtgcagg cgaaggcatc tttatggcct tccagtatcc ggtggagatt
     8041 cccggcgtca gtaaccagtt tttcctgcaa acggcactta atgcggtgcg cagctatcgc
     8101 ggccaggaaa cgctcgaccg ctttgatttt caggatttga tggaagagaa aatcgctctc
     8161 ctgaagatgc cggaagattt attaacccgt tcggtaaacg ttggtttttc cggcggcgag
     8221 aaaaagcgca acgatatttt gcaaatggcg gtgctggaac cggagttatg cattcttgat
     8281 gaatcggact ccgggctgga tattgacgca ttaaaagtgg ttgccgatgg cgtgaactcg
     8341 ctgcgtgacg gcaagcgctc attcatcatt gttacgcact accaacgcat tctcgactac
     8401 atcaagcctg attacgtcca tgtgctatat cagggacgaa ttgtgaaatc cggcgatttc
     8461 acgttggtca aacaactgga ggagcagggt tatggctggc ttaccgaaca gcagtaatgc
     8521 gctgcaacag tggcatcact tgtttgaagc tgaagggaca aaacgctcac cgcaagcaca
     8581 gcagcattta caacaattgc tgcgtaccgg actgccgaca cgtaaacatg aaaactggaa
     8641 atatacgccg ctggaagggc tgaccaatag tcagtttgtc agcattgcgg gagatatatc
     8701 cccacagcag cgtgatgcct tagcgttaac gttagacgcc gtgcggctgg tgtttgtcga
     8761 tggacgttac gtgcccgcgc tgagcgatgc aattgaaggc agcggatatg aagtgagcat
     8821 taacgacgac cgtcaggggt tacccgacgc tattcaggcg gaagtgtttc tgcatttgac
     8881 ggaaagcctg gcacaaagcg tgacgcatat cgccgtgaag cgcggtcaac ggccggcaaa
     8941 gccattgctg ttaatgcata tcacccaggg cgtggcaggt gaagaggtga acactgccca
     9001 ttaccggcat catctggatc tggcggaagg tgccgaagca acggtgatcg aacattttgt
     9061 cagccttaat gatgctcgtc attttaccgg ggcacggttc actatcaatg tcgcagcgaa
     9121 cgcccacttg cagcatatca agctggcgtt tgaaaacccg ctcagtcacc actttgctca
     9181 taacgatttg ttgctggctg aggatgccac cgcatttagc catagtttcc tgctgggtgg
     9241 cgcagtgtta cgacacaaca ccagtacgca actcaatggc gaaaacagca cgctgcggat
     9301 caatagcctg gcgatgccgg tgaaaaacga ggtgtgtgat acccgtacct ggctggaaca
     9361 caataaaggt ttttgtaaca gccgacagtt gcacaaaact atcgtcagcg acaaaggccg
     9421 cgcggtattt aacggtttga tcaacgtcgc gcagcacgcc atcaaaacgg atggtcagat
     9481 gaccaacaat aatctgctga tgggcaaaat ggcggaagtg gatacaaaac cgcagctgga
     9541 aatctatgca gatgatgtga aatgcagcca cggcgcgacg gtggggcgta ttgatgatga
     9601 acagatgttc tatctgcgct cgcgcgggat caatcagcag gatgcccagc agatgatcat
     9661 ttacgccttt gctgccgaac tgacggaagc actgcgtgat gaggggctta aacagcaggt
     9721 gctggcccga atcggtcaac ggctgccagg aggtgcaaga tgactttttc cgtcgacaaa
     9781 gtgcgggccg actttccggt gctttctcgt gaggtgaacg gtttgccgct ggcttatctc
     9841 gatagcgccg ccagtgcgca gaaaccaagc caggtgattg acgccgaggc tgagttttat
     9901 cgtcacggct acgcggcggt gcatcgcggt attcatacct taagcgccca ggcgaccgag
     9961 aaaatggaga acgtgcgcaa gcgggcatcg ctgtttatta atgcccgttc ggcggaagag
    10021 ctggtgttcg tccgcggcac gacggaaggg atcaatctgg tcgccaatag ttggggcaac
    10081 agcaacgtgc gggcgggcga taacatcatc atcagtcaga tggagcacca cgctaacatt
    10141 gttccctggc agatgctttg cgcacgcgtt ggcgcagagc tgcgtgtgat ccctctcaac
    10201 cccgacggta cgctgcaact ggagacgctg cctacgctgt ttgatgagaa aactcgcctg
    10261 ctggcaatta ctcatgtctc caacgtgctg ggaacagaaa atccgctggc ggaaataatc
    10321 acgcttgcgc accagcatgg cgcaaaagtg ctggtggatg gcgctcaggc ggtgatgcat
    10381 catccggtgg atgttcaggc gctggattgc gacttttacg tgttctccgg gcataaactg
    10441 tatggcccca ccggaattgg cattctttat gtgaaagaag ccttgttgca ggagatgccg
    10501 ccgtgggaag ggggcggttc tatgatcgcc accgtcagcc tgagtgaagg cactacctgg
    10561 accaaagcac catggcggtt tgaagccggt acacccaata ccgggggcat tattggtctt
    10621 ggcgcggcgc tggagtatgt ttcggcgctg gggcttaata acatagccga gtatgaacag
    10681 aatctgatgc attatgcgct atcacagctg gaatctgtac cggatctcac tctctatggc
    10741 ccacaaaaca ggcttggcgt tattgctttt aatctcggta aacaccacgc ctatgatgtt
    10801 ggcagttttc tcgataatta cggcattgct gtgcgtaccg gacatcactg cgctatgcca
    10861 ttaatggcct attacaacgt ccctgcgatg tgtcgggcgt cgctggccat gtataacacc
    10921 catgaagaag tggatcgtct ggtgaccggc ctgcaacgta ttcaccgttt gctgggataa
    10981 cagggaggca ctatggcttt attgccggat aaagaaaaat tgctgcgtaa ttttttacgc
    11041 tgcgccaact gggaagagaa atatctctac attattgagc tgggccagcg tctgactgaa
    11101 ttacgcgacg aagaccgaag cccacaaaat agcattcagg gctgccagag tcaggtgtgg
    11161 attgtcatgc gccagaatgc gcacggaatt attgaattgc agggcgacag cgatgcggcg
    11221 attgtgaaag ggcttattgc ggtagtcttt attctctacg atcagatgac gccgcaggat
    11281 attgtcagtt tcgatgtgcg tccgtggttt gaaaaaatgg cactcaccca acatctcacc
    11341 ccatctcgtt cacaaggtct ggaagcgatg attcgcgcaa ttcgcgccaa agccgctgca
    11401 cttagctaaa ctaaagggac agctttcatc cggttggctc acattgccgg atggtcaatg
    11461 tttacactga cctgcacagg agtgttttgg tttcaggtga acataagcgc catccgacgt
    11521 ttcagcgtga gtctccggca aatacaggag gtttacaatg aaacgcgcgt ctttgcttac
    11581 actcacgctt atcggcgctt ttagcgccat ccaggctgcc tgggcggttg attatccgct
    11641 accaccaacc ggaagccgac tggttgggca aaatcaaacg tatacggtgc aagaagggga
    11701 taaaaacctt caggctattg cccgacgttt tgatactgcg gcaatgttga tccttgaagc
    11761 caataacact atcgtcccgg tgccaaaacc tggtacgacg ataactattc cttcacaact
    11821 gttattacct gatgcaccgc gtcaggggat tatcgttaac cttgcagagc tgcgccttta
    11881 ttattatccg ccgggagaaa atattgtgca ggtttatcca ataggtattg gattgcaggg
    11941 gctggaaacg ccggtgatgg aaacgcgtgt tgggcagaaa atccctaacc caacctggac
    12001 gcctacggca ggcattcgtc agcgttcgct ggagcgtggc attaaattac cgccagtcgt
    12061 tcctgccgga ccaaataacc cgctaggacg ttacgcactg cgcctcgcgc atggtaatgg
    12121 cgaatacctc attcatggca ccagtgcgcc ggacagcgtt ggtttgcgcg tcagttcagg
    12181 gtgtattcgc atgaatgcac cggatattaa agccttgttc tccagcgtgc ggacgggaac
    12241 gccggtgaaa gtgatcaacg aaccggtgaa atattccgtg gaacctaacg ggatgcgtta
    12301 tgttgaagta catcgaccac tatcagcaga agaacagcaa aacgttcaga caatgccata
    12361 cacactgcca gcaggcttta cgcaatttaa agacaataag gctgtagatc agaagttagt
    12421 cgataaagcg ttgtatcgtc gggcagggta tccggtttcg gtgagcagtg gagcaactcc
    12481 cgcagccagc aatgcgcctt cagtagagtc agcgcagaat ggtgaaccag agcaagggaa
    12541 tatgttacgc gcgacgcagt agtggtaaat agcagacaaa aaaatggcgc acaatgtgcg
    12601 ccatttttca cttcacaggt actattactt gcggtattta gtagccatgt tgtccagacg
    12661 ctggttagca cgagctgcgt catctttagc agcctgaacg tcggaacgca ttgcgttcac
    12721 gtcgttgctc agctggtcaa ctttagcgtt cagagtctga acgtcagaag acagctgatc
    12781 gattttagcg ttgctggagc aacctgccag cagagtagaa cccaggatta ccgcgcccag
    12841 taccagttta gtagctttca ttattaatac cctctagatt gagttaatct ccatgtagcg
    12901 ttacaagtat tacacaaagt tttttatgtt gagaatattt ttttgatggg aaggcactta
    12961 tttttgatcg ttcgctcaaa gaagcatcga acgctgggtt ttagataaaa aaactgagag
    13021 aaaacaggat gcttccatcg gattcatctt agataaagtg agattatata gtgaaatccg
    13081 atttgatttt ttattgcttc tggttatcga ttaaataaaa aagcgcccat cagggcgctt
    13141 cgatatacaa attaattcac aaaagcaata ttacaggacg tgaacagatg cggtgttagt
    13201 agtgccgctc ggtaccagtg caccagaaac cataactacg acgtcacctt tgtgtgccag
    13261 accgctctgc agagccagtt ctttacccag acggtagaaa tcatcagtag aagtgatctc
    13321 tttaaccagc tgcggcacaa cgcctttgct cagtaccaac tgatgagccg ttttttcgtt
    13381 ggtggtcagt gccagtatgg tggcatccgg gaagtattta cgtactgcgc gagcagattt
    13441 accgccctgg gtagcaacca cgatcagcgg agcatccagt ttttcagcag tttcaacggc
    13501 accacggcat accgcttcgg taatgcgcag tttacggttg tcattgttga actcgagacg
    13561 gctgttcatc acgcggtcgg tacgttcgca gatggtcgcc atgatagaaa ccgcttccag
    13621 cgggtattta ccttttgcgg attcaccaga cagcatcact gcgtcagtac cgtcgaggat
    13681 ggcgtttgca acgtcaccgg cttctgcgcg agtcgggcgt gggtttttga tcatggaatc
    13741 cagcatctgg gtcgcagtga taacgacttt acgtgcacgg atacattttt cgatcatcat
    13801 cttctgggcg aagataactt cttctaccgg aatttctaca cccaggtcgc cacgcgcaac
    13861 catgatgccg tcagaggctt cgaggatttc gtcgaagttg ttgaggcctt cctggttttc
    13921 gattttggag atgatgtgga tgttttcgcc gccgtgcgct ttcaggtgct cacggatttc
    13981 gataacgtca gaacgcttac gaataaagga agcagcaaca aagtctacgc cttgttcgca
    14041 accaaagatc aggtcctgtt tgtctttttc agccagtgct ggcagggcaa tggaaacgcc
    14101 aggcaggttc acacctttgt tttcgcccag gtcgccgttg ttcagcactt tacagataac
    14161 tttgttacct tcaatggcag taacttccat accgatcaga ccatcgtcaa ccagtacggt
    14221 gttgccaaca gacaggtcag tagtgaaacc ttcatacgtt accgcaacca tttcgctgtt
    14281 gccgataaca gatttatcag tggtgaaagt aaaggtctga cccgctttca gagaaacgtc
    14341 gttaccgcct tccagtttca tggtgcggat ttccggacct ttggtatcaa gcaggatagc
    14401 ggcggtttta ccagttttgc tcatcacgtt gcgcagattc tgaatgcgct gaccgtgttc
    14461 tgcatagtca ccatgagaga agttcagacg cataacgttc atgccagcgt ccagcatttt
    14521 agctaacatc tcttcagatt cggttttcgg tccgatggtg caaacaattt tggtcttttt
    14581 catgacagtc ttagtcttta agttgagaag gatgggagaa acttgctttc tgggcgcact
    14641 gccggaaagc gaatccggta ttacgaaagt ggtggtgcct tgctctaagg ataggtgaca
    14701 tcgaaaaagc gtgcagagga atgtgtgctt gtgtctcagc ccaacgtgag tgcctgtttt
    14761 tgagtcgttg agttctacag tccaaagtgc tgaaaccatt caagagtcaa ttggcgcgca
    14821 ttatacgctg aaattaatcc aaaaggaaaa caaaaacagc gtttcaaaaa atatacaact
    14881 tgttagacaa taaattgtta tcaaagattt aacgttccag ggaaaaggtt acgcaaaaac
    14941 ttcgctgttt gcgcaacgtc tggtgccgaa gttgtcagtg tgaggataag tgaaagttca
    15001 tccggtgtat aaatatatca tcgctcaatt gctacagtta aaataattta tcgcgttatt
    15061 tgtctgattt tgtttgattt ttgtttaatt ggttagattg atatatgctg atagttggca
    15121 tgataaatag gtataaagaa tgggaaatcg gacaaaagag gatgaactgt accgagaaat
    15181 gtgcagagtt gtcggtaaag ttgtgctgga aatgcgcgat ctggggcagg aaccaaagca
    15241 tattgttatc gcaggcgtat tacgtactgc attagccaat aaacgcattc agcgcagtga
    15301 gctggaaaag caggcgatgg aaaccgtcat taacgcactg gtaaaatgac cattcaggat
    15361 gaggcggtaa gcttcatcct gatattcttg cttcagttgc ggtccagtta atcattctaa
    15421 ataacaactg ctgaaattat acgaattgcg gtatgtattt taatctataa cgataatatt
    15481 atttttattt tctgcaatga acgtcacaaa taattgtcgc ttaagttaaa tttatttcaa
    15541 ttgataataa atatcagttc acttataatt tcttgctttg taattttaat tgataacgat
    15601 caatgttaag ggctcagaat aatcacatta ttttttctcc taaccataaa ggattggttt
    15661 atcggcgtta aatcttttct catacactat cgggttaggg aaaatatctg gtgttgggga
    15721 cagagtcagc agcgtattag ttgaatgctt aatatcaggc tgctgaattg tcagcactgt
    15781 gtttgcgaag aggatgcgaa ataaatgaac ccggttgatc gtccactatt agatattggc
    15841 ttaacgcgat tagaattctt gcgtatatca ggaaaaggcc ttgctggtct aacaattgcc
    15901 cctgcgttac tgtcactttt gggttgcaaa caggaagata ttgatagcgg cacagtaggg
    15961 ttgataaata cacccaaagg ggtgctggtg acccagcgag cgcgatgcac gggttgccac
    16021 cgctgtgaaa tctcttgtac caacttcaac gatggctcag tagggacatt cttctcccgt
    16081 atcaaaatcc atcgcaatta tttctttggc gacaacgggg ttggctctgg cggcggcctg
    16141 tatggcgatc tcaactatac cgcggacacc tgccgtcaat gcaaagagcc gcaatgcatg
    16201 aacgtctgcc cgattggtgc gattacctgg cagcagaaag aaggctgtat taccgtcgat
    16261 cataaacgct gtattggctg tagcgcctgt actacggcat gcccgtggat gatggccacc
    16321 gtaaataccg aaagtaaaaa atcctcgaaa tgtgtgttat gcggtgaatg cgcaaacgcc
    16381 tgcccaacag gggcgttaaa aattatcgag tggaaagata ttactgtgtg aatcttgcaa
    16441 aggaaaaaat catggctaac ggttggacag gtaatatatt aagagtcaat ctcacgacag
    16501 gaaatattac cctcgaagat tccagtaagt ttaaaagttt tgtcggtggc atgggcttcg
    16561 gctacaaaat tatgtatgac gaagtaccgc caggcacgaa acctttcgat gaagcgaata
    16621 aattagtctt tgctaccggc ccattaactg gatctggtgc cccctgtagt tctcgcgtaa
    16681 atatcacctc actttctact tttaccaaag gaaatttagt cgtcgatgcc catatgggtg
    16741 gcttttttgc agcgcaaatg aaattcgctg gatacgacgt cattattatc gaagggaagg
    16801 cgaaatcacc ggtatggctg aagattaaag atgacaaagt tagcctggaa aaagccgatt
    16861 tcttatgggg aaaagggacg cgcgcaacga cggaagaaat ttgtcgattg accagtccgg
    16921 aaacctgtgt ggcggctatt ggtcaggctg gggaaaacct tgttcctctc tctggcatgt
    16981 tgaatagccg taaccacagc ggcggtgcgg gaactggcgc aataatgggt tcgaaaaacc
    17041 tgaaagcgat tgcggttgaa gggacgaaag gggtcaacat tgccgatcgt caggagatga
    17101 agcgtctcaa cgattacatg atgactgaac ttattggtgc gaataacaac catgtcgtgc
    17161 caagtacgcc gcaatcgtgg gcagagtatt cagatcccaa gtcacgttgg acagcacgta
    17221 aagggctgtt ttggggcgcg gctgaaggtg gtccgattga aacgggggaa attccgccag
    17281 gcaatcagaa cacggtcggc tttcgtacct ataaatccgt ttttgactta ggtccggcgg
    17341 cagagaaata cacagtaaaa atgagtggct gccactcttg cccgatccgt tgtatgaccc
    17401 aaatgaatat tcctcgggtg aaagagtttg gcgtgcccag cacaggtggt aacacttgtg
    17461 tagccaactt tgtccatacc accatcttcc cgaacggtcc gaaagatttt gaagataaag
    17521 acgatggtcg tgtgattggt aacctggtgg gtctgaatct gttcgatgac tacggcctat
    17581 ggtgtaacta cgggcagttg catcgcgact ttacctattg ttacagcaaa ggtgtgttca
    17641 agcgtgttct gcctgctgaa gagtatgcgg aaattcgctg ggatcaactg gaagcgggtg
    17701 acgttaactt cattaaaatt aaagattttt actaccgtct ggcgcatcgt gtgggtgagc
    17761 tgagtcacct ggctgatggt tcatatgcca tcgcagaacg ctggaatttg ggtgaagagt
    17821 actggggcta cgcgaaaaat aaactctggt cgccgtttgg ctatccggtt caccatgcca
    17881 atgaagcgtc agcgcaggtc ggttccattg ttaactgtat gttcaaccgt gactgcatga
    17941 cgcataccca tatcaacttt at
//
LOCUS       DAJVRF010000080        17853 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJVRF010000080 DAJVRF010000000
VERSION     DAJVRF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.080"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..304)
                     /locus_tag="OQN13_002488"
     CDS             complement(<1..304)
                     /locus_tag="OQN13_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCS1615822.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
     gene            complement(514..1032)
                     /locus_tag="OQN13_002489"
     CDS             complement(514..1032)
                     /locus_tag="OQN13_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCS1615823.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1729..2229
                     /gene="tssB"
                     /locus_tag="OQN13_002490"
     CDS             1729..2229
                     /gene="tssB"
                     /locus_tag="OQN13_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HCS1615824.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            2264..2488
                     /locus_tag="OQN13_002491"
     CDS             2264..2488
                     /locus_tag="OQN13_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1615825.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            2539..4014
                     /gene="tssC"
                     /locus_tag="OQN13_002492"
                     /pseudo
     CDS             2539..4014
                     /gene="tssC"
                     /locus_tag="OQN13_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            4021..4434
                     /gene="tssE"
                     /locus_tag="OQN13_002493"
     CDS             4021..4434
                     /gene="tssE"
                     /locus_tag="OQN13_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCS1615826.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            4438..6288
                     /gene="tssF"
                     /locus_tag="OQN13_002494"
     CDS             4438..6288
                     /gene="tssF"
                     /locus_tag="OQN13_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCS1615827.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            6252..7334
                     /gene="tssG"
                     /locus_tag="OQN13_002495"
     CDS             6252..7334
                     /gene="tssG"
                     /locus_tag="OQN13_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HCS1615828.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            7359..8639
                     /gene="tagH"
                     /locus_tag="OQN13_002496"
     CDS             7359..8639
                     /gene="tagH"
                     /locus_tag="OQN13_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HCS1615829.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            8636..9160
                     /gene="tssJ"
                     /locus_tag="OQN13_002497"
     CDS             8636..9160
                     /gene="tssJ"
                     /locus_tag="OQN13_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HCS1615830.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            9163..10492
                     /gene="tssK"
                     /locus_tag="OQN13_002498"
                     /pseudo
     CDS             9163..10492
                     /gene="tssK"
                     /locus_tag="OQN13_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            10497..11258
                     /gene="icmH"
                     /locus_tag="OQN13_002499"
     CDS             10497..11258
                     /gene="icmH"
                     /locus_tag="OQN13_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HCS1615831.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            11267..14050
                     /gene="tssH"
                     /locus_tag="OQN13_002500"
                     /pseudo
     CDS             11267..14050
                     /gene="tssH"
                     /locus_tag="OQN13_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
     gene            14047..14790
                     /gene="tagO"
                     /locus_tag="OQN13_002501"
     CDS             14047..14790
                     /gene="tagO"
                     /locus_tag="OQN13_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HCS1615832.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            14795..16207
                     /gene="tssA"
                     /locus_tag="OQN13_002502"
     CDS             14795..16207
                     /gene="tssA"
                     /locus_tag="OQN13_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HCS1615833.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDSRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIH
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            16226..>17853
                     /gene="tssM"
                     /locus_tag="OQN13_002503"
     CDS             16226..>17853
                     /gene="tssM"
                     /locus_tag="OQN13_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HCS1615834.1"
                     /translation="MFRLPTPRLLSGLKSALRPAMPRFKVSAFWLLILAWIFLLVWIW
                     WKGPMWTLYEEQWLKPLANRWLATAAWGIIALVWLTVRVMKRLQQLEKMQKQQREEAV
                     DPLSVELNAQQRYLDRWLLRLQRHLDNRRFLWQLPWYMVIGPAGSGKTTLLREGFPSD
                     IIYAPEGARGAEQRLYLTPHVGKQAVIFDIDGTLCAPADADILHRRLWEHALGWLKEK
                     RARQPLNGIILTLDLPDLLTADKRRREHLLQTLRSRLQDIRQHLHCQLPVYVVLTRLD
                     LLQGFAALFQSLNRQDRDAILGVTFTRRAHENDDWRTELNAFWQTWVDRMNLALPDLM
                     VAQTHTRASLFSFSRQMQGSREPLVSLLEGLLDGENMNVMLRGVYLTSSLQRGQMDDI
                     FTQSAARQYRLGNNPLASWPLVDTAPYFTRSLFPQALLAEPNLATESRAWLIRSRRRL
                     TVFSATGGVAALLLITGWHHYYNGNYQSGITVLKQAKAFMDVPPPQGEDDFGNLQLPL
                     LNPVRDATLAYGDWGDRSRLADMGLYQGRRIGPYV"
BASE COUNT         4154 a         4895 c         4877 g         3927 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 tcgtcaggta ggccattttg tccagtacct gcggaaattc aagggagaga aactgctggc
      181 tgaccagaga gagctcgagg gaaaagagcg aagagagtga ctggttcaga tgaaaggaga
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag cgtgaaacgt aatccggttg
      301 acatagcaac ctccggggtt aaggaaaaac agacagttcg ctgttaaagc ggctgtgaca
      361 gtacagtgtt acgcttgcgt tcaggcacgc taccgccgaa gtttccggca tccgccatct
      421 cccgtgaacg taacccctta ctgacaacct ttctttctac ggcgtaatac aaaaacagca
      481 tccggcctga tagccggatg ctgttggctt aacttatgct tccagtggtg cgcgccagtc
      541 gtcggcaccg gaggtgccag atttgacgtg ttcccattca attttgcggt aggccagtga
      601 aacctcgagc aactgggtga actcgcgttt cgccgggtcc tggcaatgcg gcatatgcag
      661 tttcatatcg acaatcgtcg aatcagtcag gcgggtagtg aaataatgct cctgtttgcc
      721 ctccacagag gtacgccacc agtgaagttc agtggttggc agcatttcgc cggaggccag
      781 tgcgttgtac atcagcggaa ccgctttgtt cagcgccacg gtaaaaatga aaggcttgtg
      841 agcacgctga cccgcaggct gaccggactg aggagtggtc gggacagtaa cgttatgcag
      901 gaattcctgc accagcatct cgtcttcgtg cccctgcaca tagatgttgc cgacagaatc
      961 agcggtaaat gcacctgcag taatatttcc ctgggtctga ccagtgatgg agatataaca
     1021 cggtgttggc atttgtaaac tccttgttaa cgtggttaat tactacttac aatgacctgc
     1081 cgttaatccc agacaggcag atgcagctca cttcacggaa ccgcatcatt tttcagtatt
     1141 ttttacatac aaaccgagct agaatttacg gttgtcgtaa aaactatttt taaaatcatt
     1201 aaatctctta ttcagcaata acttaataat atatatctgg ataaccatac ccaactgttc
     1261 aaagtccgta ttgcctccat aaaatggcca aagctgaatc atgaataaat attgtattta
     1321 tcctttctga attatcattt tgataatttt tgagatattc cttaaaaata cccacttaat
     1381 caatcacaaa acaaatatat aaacaagcaa tcattagatt agcttatttt taatgaaatt
     1441 attatatact tcaataagtt acacaaaaac tctatattta aattattgcc ataatacacc
     1501 tgaagcgaat tgcacaggtt cacacaatct atttaacgct tgcagaatac ccctctttac
     1561 gatcgataaa aagtcctttt cttatttatt ttgaagcgta aaatgcgggg tgatgaagga
     1621 gtggtgattt tattttcacc gccattaggg cggcattgcc gatatttttt tgcggtacaa
     1681 atatgccgtg ttcatggcct gtacgagata acatgacgga tcacttttat gagcaaaaaa
     1741 tttgaaggca gcgtcgcgcc ccgcgagcgt attaatatat cgtatgtccc aaaaacagat
     1801 ggccagacgg ctgaagtgga acttccgctt aacatgctcg ttgtcggcga taccggaaat
     1861 acgcaggaaa catccccact ggatgaacgt caggcagttt ccgttaacaa gcacaatttc
     1921 ggtgcagtga tggctgaagc agccattggc ctcaatttta ctgtccccgc cacgctgaag
     1981 ggaagcacaa cagacgatga actgaatgtg gcgctgaata tcaaatcact ggatgatttt
     2041 tcaccagata gcgtggcccg gcaggttccg gaagtgaata aattgctgga actgcgtgaa
     2101 gcactgactg cactgaaggg gccgatgggt aaccttccgg cattccgtac ccagctccag
     2161 gcattgctgg aaaatgaaga gtcccgcgag cagttgctca aagaaatcgg tctggtgagc
     2221 aataaataac caccactatt tcattaaaca gggttatcca ggcatgacgg cacacaaaaa
     2281 tatctccggg acataccacc tctctgttgc agacattctg caggtggttt accaggtatg
     2341 tttcagcccg tccgttgaaa tcaaccagga tggtgtggct gcgctgatta caacactgga
     2401 tcgccgtatc agtgatctat tggatgagat tatccatttt tgtgagtttc agcagagcgc
     2461 gtcgcactgg cagcgcgtac ttcactgatg gcgaaaagcc ggtttcagaa acacagctaa
     2521 aacagaagga ttttcagtat gtctttacag gaagaggaac tcgtttccag tcatgccggg
     2581 cagcctgagc aggcatcgtc gctgcttgat caaatcatgg cacagacccg tattcagccc
     2641 ggttcggaag gttatgacgt tgccctccag ggtgtgacag cctttattgc cagcatcctg
     2701 caatccactg catctgccga accggtcaat aaactggccg tggacagcat gattgcagat
     2761 attgatgaac gcatcagccg ccagatggat gtcatcatcc atgcgcctgc ctttcagcag
     2821 gtcgagtcgt tctggcgctc actgaaaacc atggtggatc gcgttgattt ccgtgaaaac
     2881 atcaaggtca acgtgcttca tgtcaccaag caggaacttc tggaagactt tgagtttgcg
     2941 ccagaaatta ttcagtccgg attttataag cacgtttatt cgtccggttt cggacagttt
     3001 ggcggtaaac ccatcgctgc cgtgctgggc gcttatgagt tcaaaaatac cgcgcctgac
     3061 atgaaactgc tgcaatacgt cagtgccgtg ggcgcaatgg cgcacgcacc gttcctgtcc
     3121 tccgtttccc cggagtttat ggggctgaac tcgtggaccg aactgcctaa tatcaaagat
     3181 ctttatgcca tctttgaagg tccggcctac accaaatggc gcgccctgcg tgactcggaa
     3241 gattcgcgct atttagggct tacggcaccc cgcttcctgc ttcgccagcc ctattcacca
     3301 acagataacc ctgttaagaa ctttaactat tacgaagatg tcagccagaa ccacgaagat
     3361 tatctgtggg gtaatacggc ctggatgctg gcatgcaata tcgcagacag ttttgccaaa
     3421 taccgctgat gtccaaacat tatcggtccg caaagcggcg gcgcagtgaa agatctgccg
     3481 atgcatctgt tcgaaacgat ggggcagatt caggccaaaa tcccaaccga ggtgctggtc
     3541 accgatcgcc gcgaatttga actggctgaa gagggtttta tcacccttac catgcgtaaa
     3601 gactctgata atgcagcctt tttctctgca aactcggtac aaaaaccgaa gcactttccg
     3661 ggaaaagatg cagaaaccaa ctataagctg ggtacgcagc ttccttatct cttcatcatc
     3721 aatcggttag cgcactacat caaggtgttg cagcgtgaac aactggggtc atggaaagaa
     3781 aggagcgact tagaaagaga gcttaatacc tggatccgtc agtatgttgc cgaccaggaa
     3841 aacccgcctg ctgacgtgcg cagccgcaaa cctctgcgcg ctgccaaagt tgaggttatg
     3901 gatgtagaag gcgaacccgg ctggtaccag gttgcgctaa gcgtgagacc tcattttaaa
     3961 tttatggggg caaattttga gctttccctg gttggccggt tagacaggga gtaacacacc
     4021 atgataccgg atcgggaaag aaccacaggt tctctttttg agcgtatgga agcctcttcg
     4081 gccagaaacc ggcagggagg cagtattcat tcgctcaggc aatccattcg ccagaaccta
     4141 cgaaacattc ttaatacccg gtccggtagt tgccggggtg cgcctgagct gggcattgat
     4201 gaaccggaag gggctgaaaa cttccgcgaa tccatgagtc gggccatcga gcaatgtata
     4261 gaacgctatg aaccccggat ctcacacgct gaagtgcagg cagtggtttc atccgcctcc
     4321 agcccgctgg atatgacgtt tcatattaca gcctgggtga catttaacga aacgcatgag
     4381 gtgcttgaat ttgatatggc accgaatggc agccagcatt accgggtgga ctgacaaatg
     4441 gaatttgaag aacgctattt ccgggaagaa ctcgattacc tgcgccagct tagcaagctg
     4501 ctggcaacgg aaaaacccca tctggcccgc ttcctggccg aaaaagatgc agatccggat
     4561 attgaacgcc tgctggaagg ggtggctttt cttaccggca atctccgcca gaaaattgag
     4621 gatgaattcc cagaactgac gcacgggctt attaagatgc tatggcctaa ttacctgcgt
     4681 ccggttccgg caatgaccct tattgaatat acgccggata tggataagtc ttctgtaccg
     4741 gtgttaatcc cccgtaatga gcagtttaca accaacgccg gggaaatcag agttgatgaa
     4801 gtgctgccct ctgatgctaa aaaggaggag ccgcctccct gtaccttcac cctctgccgg
     4861 gatatctggc tgctgcccgt tcgcctggag cagattgaaa accgcagtac gacccgtaat
     4921 ggtgttatca acatcacctt ttcggtcgca ccgggaacgg acttccgcac gctggatctg
     4981 aacaaacttc gcttctggct cggcaatgac gacaactata cccgtgacca gctttattta
     5041 tggttctgcg aatacttgca gggtgccgac ctgactgtgg gtgaacagca tattcgcctg
     5101 cctgagttta tgctaaaagc tgtcggtttt gagccgcagg atgccatgct gccctggccg
     5161 aaaaacgtcc acagcggcta ccggatcctt caggagtatt tctgttaccc cgatgcgttt
     5221 ctcttttttg atctttgtgg ttgtccggct ttgcctgacg gattgcaggc ggaattcttt
     5281 accctgcaac tgcgtttttc gcgccctttg cccgtggaca tccggctgcg ccgcgattcc
     5341 ctgcgcctgt attgcgcacc tgccattaat ttatttatcc accatgcaga agccatcacg
     5401 ctggacaacc ggcgggcaga ctatccgctg gttcccagcc gccattaccc acaacattac
     5461 gatgtatttt ccgttaacag tgtggtgagc caggtccagg atatgttcag gaaaaaagat
     5521 ctggggcgtc ctgtttcgac gcaggccgcg cgccagtggc cagcctttga aagtttcagc
     5581 catcagatag aatacagccg gaagcgggaa gtggtgtact ggcatcaccg gaccaaaaca
     5641 tccctgttcc atcgcggctt tgatcatacc cttgccttta tacatgctga tggcagttat
     5701 ccgtcagacg aatctctgct cagtaatgaa gtggtttcgg tatcgctgac ctgtaccaac
     5761 cgtgagcttc cgtcacaaat tcgttccggc gatatcaccg gcacaaccgg aaaaaatgcg
     5821 gctgttgctt catttcgcaa cattacccgc ccgacgcaac cactctggcc ggtcattgat
     5881 ggcagcctgc actggcccct actctccgcc atgaacctga attatctgtc attactggat
     5941 acggacgcgc tgaagcaggt catcgccaac tttgatcgcc acgcaatcca tcatccgcag
     6001 acggcacggc tgtcacaaca aaagctggat gccattgagc gtctggagac ccgccccgtt
     6061 gatcgcctgt ttacgggtat tcccgtccgg gggctggctt ccacgctgta tctgcacccg
     6121 gagccgtttg tctgtgaagg ggaaatgtat ctgctcggta cggtgctttc gcattttctg
     6181 tcgctgtacg ccagcgttaa ctcattccac atgctgaccg ttgtgaacac agaaagccag
     6241 gagacatgga aatggacgga aagaatcggg cagcatcctc ttatctgagt ccgggaaatc
     6301 ccccggcaga taaagagcag aatgatccgc tggcacaggt tttccataat gcctgctctt
     6361 acaatttttt tgcgatggcg gagctgttgc accgcctggc aaagggtgaa aaggggacgc
     6421 cagaattatc cctgcgcgac gatccggcac aggaaaccct gcgtttcagc gccgatgcca
     6481 gccttgcctt cccgtgcagt gatatcagtg cgctgaaacg ggatacgtca ggggcattca
     6541 ggatgaccac cacctttatg gggttacagg ggagccagtc gcctctcccc ggttattacc
     6601 tcgctcacct ggcctggaaa gcggtacatg aacaaagtcc ggtaggtgat tttctggata
     6661 tgttcagcca ccgcctgacc cagtttgtct ggcatatctg gcgtaagtac cgctatcaca
     6721 tcagttttcg taacggcggt gtggatgcgt tctcgcagcg catgtactcc cttgtcgggc
     6781 tgggccaccg ccagcttcgc gataagctgg cgattaatca cagcaaaatg ctggcgtatt
     6841 ccgggatcct ggcaaatccg ggacgatccc cggaaattat ctgtggcctg gtgtcccact
     6901 gctttgattt gtcagaagtg acactgcaaa actggcaacg tcgcaaggtg gatattgaac
     6961 cggatcagca aaatagtctg gggagctatt ctcttaaaaa tggcgaaaaa ctggcgggaa
     7021 gatcggtact gggcaacttt gttctgggta cacgagtgcc cgatctcagc ggaaaattcc
     7081 agctcagtat taccagcctg acccgaaaac agttcctctc ttttctgccg tccggcgaaa
     7141 acttcctgcc actgacgatg ttcgtgtcct tcatcctgcg cgatcagctt gcctgggact
     7201 tacatctggg gctggcgccg gagcaggtgg gcgcaatgcg cctgggcgat aacaaaagtg
     7261 cgctgctggg ctggaccagc ttcctcggca caccggaaga acgaccctca gtcaccatca
     7321 gggtacggtc gtaaattatg ataatcacgg agcagaatat gccggaagag aaactacaga
     7381 cgctttcatt gcaggtcatt aacggcagtg agctggaaag cgggcgggcg gcgcgctgtc
     7441 tgttcacaca gcagggaaat gtcggccatg gccccgaatg ccactggtcg gtacaggatc
     7501 gtcagcagag cattccggcc caggctttta ccgttatcct gcacgatggc acattttgtc
     7561 tgcgcccgca gacggcacaa ctgtggctga atcaggcaaa agtcacagca acatcagacc
     7621 taatacagtt gcgccagggc gatgagatcc agatcggacg gctgatggtg agggttcatc
     7681 tgaaccgggg agatattccc cattacgatg aggaaatggc cactcccgaa accatcgtta
     7741 ccaatcgcga tatgctcacg gataccctgc tatcaacgga gggtgcgcca cactatccgg
     7801 gaatgactca ccggcaccag cttgcagaca ccgtggtaaa cggtttttct gccgatccac
     7861 tccaggctct tcagtccgaa agcctgatta ccacgggcga tccgctttca ggcattgcgg
     7921 ctgtccggcc atcggcaccg ctgtccgatc cggcaagtaa tggggggatc aatactccgt
     7981 ttatggatct gccgcccatt tatgccagcc ccggcgatcg taatgacgac gtttctgcgg
     8041 cagaaatggc acaacgccac gttgcggtca cccccttact gcgcggtctt ggcggctcgc
     8101 ttaccatgag caattccgac gatgcggatg attttctgga ggaggccgga cgaacgttac
     8161 aggccgcaat aaaaggtctg ctcgatttgc agcagcagcg taacagcctc tcagacaaac
     8221 atttgcgccc gctggaagat aacccgctgc gcctgaacat ggattacgcc accgcgctcg
     8281 acgtaatgtt tgccgaaggt aaaagcccgg tacatctggc ggctcccgcc gccgtcagtg
     8341 aaagcctgcg caatgtccgc caccacgaag aagctaaccg ggcagcgatt gtggagtcgc
     8401 ttcgtgtcct gctggatgct ttctcaccac aaaatctgct gcgccgcttt gtgcagtacc
     8461 gccgcagcca tgaactgcgc cagccgctgg atgatgccgg agcatggcaa atgtacagcc
     8521 attattacga agaactggcc tccgatcgcc agcaggggtt tgagatgctg tttaacgagg
     8581 tctacgccca ggtctatgac cgggtgcttc gtgaaaaaca gcgggagccg gaagcatgaa
     8641 caggaaggcg tttctggcct gcgtgctgat gtgcgtcata ttaaccggct gcgaaacggc
     8701 gaaaaaaatc agtgaggtga tcaaaaaccc ggatatccag gtcgggagcc tgaaggagca
     8761 accttcggaa ataaccgtca ccctgctgac cgaaccggac accaatacca atgcggaagg
     8821 tgaatcagcc gccgtggatg tgcaactggt gtacctgact gacgactcaa aattgctggc
     8881 agccgactat gaccagatag ccagcacgcc gctaccggat gtgctgggta aaaactatat
     8941 cgaccaccag gatttcaacc tgttgccgga caccatcaaa accctgccgc cggtaaagct
     9001 ggatgagaaa acgcagttta ttggtgtcgt ggcctatttc tccgatgacc aggccactga
     9061 atggaaacaa atcgagacgg tggaaggaat cggccaccat taccgcctgc tggtgcatgt
     9121 acgtcaaagc agcatcgaaa tgaaaaaaga ggatgaataa ttatggcaac cacccgcaac
     9181 aaggtgatgt ggcaggaagg gatgctgatg cgtccacacc atttccagca gcagcagcgt
     9241 tacaacgact acctggataa ccagcgtttc cgggccatga atgatttatc ctggggattt
     9301 accgaactca ccctcaacaa tgaactgctg gcgcagggta agatcatgat tgacagcgcg
     9361 tcaggcacac tgcccgacgg caccgtcttt tctatccccg accaggacgc actgcccgat
     9421 ccgctgcacc cgcaaaattt tccggacgaa agaagccgca atatctacct cgctctgccg
     9481 gtcgccagtg atgtgagaaa tgaaatcagc gacggacggc gaatcgggcg ttaccggctg
     9541 aattatgccg atgtccggga tttgcattca gaagaaggtg acgcgcgaac attgacgctg
     9601 ggtcaactga cgccgcgcat tatgagcggt gcagaagata tgagcgccta tattacgctc
     9661 ccgctttgcc gtatcagcga tcgccgccga tggctccctg acgttggatg acgattttat
     9721 cccctcctgc cagaatattc aggtcagtaa gaaactgcgt gtttatctca aagaggtaca
     9781 gggggccatt ggcggacggg caagcgatct ggcaaaccgt attggctctc cggcgcagag
     9841 cggcatcgcg gatgtggcgg aatttatgat gttgcagtta cttaaccgta accagacccg
     9901 gtttacccat cacgcccgtc gatcccagct ccacccggaa gatttctacc ttgatcttgc
     9961 cgggttgctg ggtgaactga tgacctttac cgagccgtcg cgcctgccct gcccgcttga
    10021 tgtgtatgat catcatgacc tgaccaaaat atttaaaacg ctgttaccgg aagtcaaacg
    10081 ggcgctgcat accgtactgt cgccaagagc cgtcaatctg ccgctgcatc tgcgtgacgg
    10141 tatctggcag gccgatatcc atgacacaga actgctgcaa tctgccacct ttgtgctggc
    10201 cgtggcggca aacatgccgg tcgatcagat ccagcgccag tttatccagc agtcgaaaat
    10261 ttcctcgccg gaaaaaatcc gcaatatggt cagtgtgcag atccccggta ttccattgcg
    10321 tgcccttatg gtggctcccc gccagcttcc ttaccattcc gggttcagct atttcgaact
    10381 cgacaagagt ggccaggcct ggacagaaat ggctgccgcc ggggccgttg cactgcatgt
    10441 ttccggcagt ttcccggatc tgaacatgca actgtgggcg ataagagggt aagacgatgg
    10501 atgaggggtc actgtctctg ccaccattta ccggacacga cgaaaaaagc cagcgcaact
    10561 accatctcgc gcttcgtggc aacagcctga atccgatgat cgacgcggcc acgccgctac
    10621 tcggtatggt aatgcgcctt tcaaccatga acagccagac gatgccggag catctttttg
    10681 ctcaggtggt gacagatgtt caggccgtgg agcaactgct tcaggagcag ggctacgaac
    10741 cgggcgtgat tatctcgttt cgctacatcc tctgcacgtt tattgatgaa gcggcgctcg
    10801 gtaacggctg gtcaaacaag aacgagtgga tcaagcagtc attgctggtc catttccaca
    10861 acgaagcctg gggtggcgag aaagttttca tcctgctgga gcgcctgatc cgtgaaccca
    10921 aacgttacca ggatctgctg gaatttctct ggatctgttt ttctctggga tttcgcgggc
    10981 gctacaaagt ggcagcacag gatcaggggg aatttgagca gatctaccgc cgtctgtacc
    11041 acgttctgca taaactgcgc ggcgatgccc ctttcccgct gttacaccag gacaaaaaaa
    11101 cccagggcgg gcgttatcag ctcatcagtc gcctgactgt taaacatatt ttttgtggtg
    11161 gcgttgtcgt gctggcgttg ttttacctgt tctatctgtt gcggctggac agccagacgc
    11221 aggacatcct gcaccagcta aacaaattac ttcgataagg acgtttatga tccagattga
    11281 tcttcccacg ctggtaaaac ggctgaacct gttctcccgc caggcgctgg agatggccgc
    11341 ctctgaatgt atgagtcagc aggcagcgga aattaccgtc agccatgtgc tcattcagat
    11401 gctcgccatg ccacgcagtg acctgcgggt tattacccgc cagggcgata ttggcatgga
    11461 agagttgcgc caggcgctga cggtagagaa ctacacaacc gcccgttctg cggacagcta
    11521 cccggcgttt tccccgatgc tggttgagtg gcttaaagag ggctagctgc tggcgtcggc
    11581 tgagatgcag cacagcgaac tgcgcggcgg cgtgttgctg ctggccctgc tgcattcgcc
    11641 gctgcgttat ataccgcctg ctgccgcccg gctgttgacc ggcattaacc gtgaccgtct
    11701 gcaacaggac tttgtgcagt ggacacagga gtcggcggaa tcagtcgtgc cggatgcaga
    11761 cggtaaaggc gcaggcacaa tgacggacgc ctctgacacc ctgcttgccc gctatgccaa
    11821 aaacatgacc gcagacgccc gtaacggcag gcttgacccg gtactgtgcc gcgaccacga
    11881 aatcgacctg atgatcgaca ttctctgccg ccgccgtaaa aacaacccag tggtggtggg
    11941 cgaagcgggc gtgggcaaaa gcgcactgat tgaagggctg gcgctgcgca tcgtggcagg
    12001 ccaggtgccg gacaagctga aaaacaccga tatcatgacc cttgacttgg gcgcattgca
    12061 ggccggggcg tcggtgaagg gtgaattcga aaaacgtttc aaagggctga tggcggaggt
    12121 catttcctcc ccggtgccgg tcattctgtt tatcgacgaa gcacataccc tgattggcgc
    12181 gggcaaccag cagggcgggc tggatatctc caacctgctc aaaccggcgc tggcgcgcgg
    12241 cgagctgaaa accatcgccg ccaccacctg gagcgagtac aaaaaatact tcgaaaaaga
    12301 tgccgccctg tcgcgccgct tccagttggt gaaggtcagc gaacccaacg ctgccgaagc
    12361 caccattatt ctgcgcggtc tgtcggcggt ctatgaacag tctcacggcg tgctgattga
    12421 tgatgacgcc ttgcaggccg ctgcgacatt aagcgagcgt tatctctccg ggcgtcagtt
    12481 accggacaaa gcgattgatg tgctggatac cgcctgcgcc cgtgtggcca tcaacctgtc
    12541 gtcgccgccg aagcaaatct cggcgctgac cactctgagc caccagcagg aggcggaaat
    12601 tcgccagctt gagcgcgagc ttcgcatcgg actgcgtacc gacacatcac ggatgaccga
    12661 ggtgctggtg cagtatgatg aaacgctgac ggcgctggat gaactggaag cggcctggca
    12721 ccagcagcag acgctggtcc gggagattat cgcgctgcgc cagcagttac tgggcgtggc
    12781 agaggacgat gcggcgccgt tgccggacgc agataccgtg gaggatacgc agccagagtc
    12841 agagtcagag tcagagtcag agtcagagca ggataatacc ggtgccgaac cggctgatga
    12901 ggccgacaga gaacaaccgg aagagaccgc tgaaacagtt tccccggtac agcggctggc
    12961 ccagctcact gccgaactgg acgccctgca taacgaccgg ttgctggtct ccccgcacgt
    13021 cgataaaaaa cagattgcgg cggtgattgc cgaatggacc ggcgtgccgc tcaaccgcct
    13081 gtcgcagaat gaaatgtcgg tcatcaccga cctgacgaaa tggctgggtg acaccatcaa
    13141 aggccaggac ctggcgattg ccagcctgca taagcatcta ctgaccgcac gcgccgacct
    13201 gcgtcgtccg ggacgcccgc tcggtgcgtt cctgctggct ggccccagcg gtgtgggtaa
    13261 aaccgaaacc gtcctgcaac tggcagagct gctctacggc ggtcgccagt acctgaccac
    13321 catcaatatg tccgaattcc aggagaaaca caccgtctcg cggctgattg gttcgcctcc
    13381 gggctacgtt ggctacggtg aaggcggcgt actgaccgaa gcgattcgcc agaaacccta
    13441 ctcggtagta ctgctcgatg aagtggaaaa agcgcacccg gatgtgctca acctgttcta
    13501 ccaggcgttc gataagggcg aaatggcaga cggtgaaggc cgcctgattg actgcaaaaa
    13561 tatcgtcttc ttcctgacgt ccaacctcgg ctaccaggta atagtcgagc atgccgatga
    13621 cccggaaacc atgcaggaag tacagtatcc ggtgctggcc gacttcttca aacctgccct
    13681 gctggcgcgt atggaagtgg tgccgtatct gccgctgtcg aaagagacgc tcgccaccat
    13741 tatcgccgga aaactggccc gtctggataa cgtgctgcgc agtcgctttg gtgtagaagt
    13801 ggtcattgaa ccggaagtga cggacgaaat catgagccgc gtcacccgcg cggaaaacgg
    13861 cgcgaggatg ctggaatcgg tcatcgatgg cgacatgcta ccgccgctct cgctgctgct
    13921 gttgcagaaa atggcggcta acacggcgat tgcccggatt cggttgtcgg cagtggacgg
    13981 cgcatttacg gcagacgtgg aagatgctca gaacgacgag tccgtcacaa aggatgaaac
    14041 ggttttatga gcaggttgcg cgttttccgg cagacaggat ggtttatcgc gggcctgatg
    14101 acgggcttac ccgccactgc cgcgcccgca gaagcatcgt caatggccgg tgtggcggtc
    14161 gccgtcgcca ccacgacccc gccggatgcg acagccacgt tacaggccat gcagtcctgt
    14221 cggcgggagt ccgcggcgct ggagcggctt gactgctatg accgcctgct ggcaccgctg
    14281 tccccgtcag ggtttgacgg ggcgctggtc aaagccggtt ttgtgggcga ggcctggacc
    14341 cgcgccacag agcaggaaaa acgccgcgag ggcaacacca cagaactgct ggtgacgcag
    14401 gtgccggggg agcgcccgac ggtggtgata accaccccgg ctatcggtca tgttccgcca
    14461 cgcccggtgc tgatgttcag ttgcgtggac aatatcaccc ggatgcaggt ggcgctgatg
    14521 caccctctgg atgtccatga tatcgccgtc actctgaatg ccgacaaccg ggcattgcgc
    14581 agccactggt ttgtgcgcga gaacggcacc ctgctggagt ccagccgggg gttatccggg
    14641 attgatgaaa ttaagcaact gttcggcgca aaaacgctga cggtggatac cggcgcagac
    14701 aatgccgccg gaaagctgac ctttaacatt gacgggctgg caagggcgat tgctcccctg
    14761 cgcgatgcct gccactgggc gggagaataa aaatatggca atggacttac gcgatccgaa
    14821 tgtctggata tcgcacctgc tggaaaacct gccggaagag aaactggcat cggcgctgaa
    14881 agatgacaac ccgaactggg agtatatcga cggcgaaatc gtcaagctgg gatcgctggc
    14941 ccacagtcaa ctggatatcc cggaactcca gcgccggggg ctggtcattc tggcttcgga
    15001 gagtaaggat tttcgtctgc tggcgcatct gctaagaacc ctgcaacacg ccggtgatcc
    15061 tcatctcgca ttgcgcctgc tggcgctgta tgtggagcat tactgggcgg tggctgcgcc
    15121 gcagaatatg gcgcataaaa aacgctttgc cagccaggtc attaaacgct ttgaaacagg
    15181 tattgaagtt ttttcacaga acgccgccac agcgcagcgc gatgcgctgt caggggaact
    15241 ggcaaaactg gcgcaatgct ggcagtcaca taacgccccg gaactggcac aggctactga
    15301 tgatcttttt gccctgtacc agcgtgcgtt taatcgtgcg gcaccggctc ctgtccccac
    15361 tccggcggcc tccggtagtt caccacagac caccgccacg tctgaaagcg gcgtgacgca
    15421 acccagtgct ccggctcccc aaatcgtcat cgacagtcac gacgacaaag cctggcgcga
    15481 cacgctgtta aaagtggcgg ccattttatg tgaacgccag ccggattcac cgcagggcta
    15541 ccgtctgcgc cgccatgcgc tttggcagaa catcaccagt acgccccagg cggaaagcga
    15601 tagccgtact ccactggctg cggtctccgc cgatatggtg gcagattacc atgctcagct
    15661 cggtagtgcc gatatggcgc tgtggcagca ggtggagaaa agcgtactgc tggctcctta
    15721 ctggctggat ggtcactgcc tttctgcaca gacggcgctg cgtctgggtt acaaacaggt
    15781 ggcagacgcc atccacgatg aggtcatccg cttccttgag cgtctgcccc agcttaccgg
    15841 gctgctgttt aatgaccaca caccgtttat cagtgagcag acgaaacaat ggctggcagc
    15901 ttcgcccgac gccaaagttg caccggttgc gcaaatcggt gaggaatcga aggcagccag
    15961 agcctgtttt gctgagcagg gtctggaggc ggcgctgcga tatctggaca tgctacccga
    16021 aggcgatccc cgcgatcagt ttcaccgcca gtaccttgcc gcacagttga cggaagaggc
    16081 ggggctggta cagcttgcgc agcagcagta ccggatgttg ttcaggatgg gattgcagat
    16141 gatggtggca gactgggagc cgtccctgct tgagcagcta gaacaaaaat tcacggcaga
    16201 acaataaaag agaacaggag tttttgtgtt cagattacca acaccccgac tactcagcgg
    16261 actcaaatca gccctgcgac cggcgatgcc ccggtttaaa gtctctgctt tctggctgct
    16321 gatactggcg tggatttttc tgctggtgtg gatctggtgg aaaggcccga tgtggacgct
    16381 gtatgaagaa cagtggctca aaccactggc gaaccgctgg ctggcaacgg cggcgtgggg
    16441 gattattgcc ctggtgtggc ttaccgtccg ggtgatgaag cgcctgcaac agcttgaaaa
    16501 aatgcaaaag caacagcgcg aggaagccgt tgatccgctc agcgtggaac tgaacgccca
    16561 gcagcgttat cttgaccgct ggctgctgcg cctgcaacgc catctcgaca accgccgttt
    16621 cctgtggcag ttgccgtggt atatggtcat cggcccggcg ggcagtggca aaacgacact
    16681 gctgcgcgag gggtttccgt ccgacattat ttatgccccg gagggcgcac gcggcgcaga
    16741 acaacgcctg tacctcacgc cccatgtcgg taaacaggcg gtgatctttg atatcgacgg
    16801 cacactctgc gctcccgctg atgcggatat cctgcatcgc cgtctgtggg aacatgccct
    16861 cggctggctg aaagaaaagc gcgcgcgcca gccgctgaac gggattattc tgacactcga
    16921 tttacccgat ctgctgaccg cagacaaacg ccgccgcgag catctgttac agacgctgcg
    16981 cagccgctta caggatatac gccagcatct tcactgccag ttaccggttt acgtggtact
    17041 gacccggctt gatttattac agggttttgc cgccctgttc cagtcactca acagacagga
    17101 tcgcgatgcg attctgggcg tcacgttcac ccgccgtgcc catgaaaatg atgactggcg
    17161 aacagagttg aatgctttct ggcagacatg ggtggatcga atgaatctgg cgttgccgga
    17221 tctgatggtc gctcagactc acacccgcgc gtctttattc agtttttccc gccagatgca
    17281 gggaagccgt gaaccgctgg tgtcactgct tgagggtctg cttgatggcg aaaatatgaa
    17341 cgtgatgctg cgtggtgtct atctcacctc ttcgcttcag cgtggacaga tggatgatat
    17401 attcacccag tctgccgccc gccagtaccg gctgggcaat aacccactgg cgtcctggcc
    17461 cctggtggac accgcgcctt atttcacccg cagcctgttc ccgcaggcat tactcgcaga
    17521 gcctaatctg gcaacagaga gccgcgcctg gctgatacgt tcccgtcgcc gcctgacggt
    17581 tttctctgcc acaggcggcg tggcagcact gctgctcatc accggctggc atcactatta
    17641 caacggtaac tatcagtccg gcatcaccgt gcttaagcag gccaaagcct ttatggacgt
    17701 gccgcctccg cagggggaag atgactttgg caacctgcaa ctgccgctcc tgaacccggt
    17761 acgcgatgcc acactggcct atggcgactg gggcgaccgc agccgtctgg ccgatatggg
    17821 actgtaccag ggacgacgta tcgggcctta tgt
//
LOCUS       DAJVRF010000081        17543 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJVRF010000081 DAJVRF010000000
VERSION     DAJVRF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17543
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.081"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..787)
                     /gene="cysB"
                     /locus_tag="OQN13_002504"
     CDS             complement(<1..787)
                     /gene="cysB"
                     /locus_tag="OQN13_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCS1615835.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFTIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAH"
     gene            complement(997..3594)
                     /gene="topA"
                     /locus_tag="OQN13_002505"
     CDS             complement(997..3594)
                     /gene="topA"
                     /locus_tag="OQN13_002505"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCS1615836.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            3974..4225
                     /locus_tag="OQN13_002506"
     CDS             3974..4225
                     /locus_tag="OQN13_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCS1615837.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4261..5310)
                     /gene="sohB"
                     /locus_tag="OQN13_002507"
     CDS             complement(4261..5310)
                     /gene="sohB"
                     /locus_tag="OQN13_002507"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCS1615838.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5530..6288
                     /locus_tag="OQN13_002508"
     CDS             5530..6288
                     /locus_tag="OQN13_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCS1615839.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6285..6875
                     /gene="cobO"
                     /locus_tag="OQN13_002509"
     CDS             6285..6875
                     /gene="cobO"
                     /locus_tag="OQN13_002509"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCS1615840.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(6915..7787)
                     /gene="rluB"
                     /locus_tag="OQN13_002510"
     CDS             complement(6915..7787)
                     /gene="rluB"
                     /locus_tag="OQN13_002510"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCS1615841.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8000..9114)
                     /locus_tag="OQN13_002511"
                     /pseudo
     CDS             complement(8000..9114)
                     /locus_tag="OQN13_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9142..9762)
                     /locus_tag="OQN13_002512"
     CDS             complement(9142..9762)
                     /locus_tag="OQN13_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCS1615842.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(9759..10640)
                     /gene="yciV"
                     /locus_tag="OQN13_002513"
     CDS             complement(9759..10640)
                     /gene="yciV"
                     /locus_tag="OQN13_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCS1615843.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            10778..10822
                     /gene="trpL"
                     /locus_tag="OQN13_002514"
     CDS             10778..10822
                     /gene="trpL"
                     /locus_tag="OQN13_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCS1615844.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    10799..10892
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            10914..12476
                     /gene="trpE"
                     /locus_tag="OQN13_002515"
     CDS             10914..12476
                     /gene="trpE"
                     /locus_tag="OQN13_002515"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCS1615845.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12476..14071
                     /gene="trpD"
                     /locus_tag="OQN13_002516"
     CDS             12476..14071
                     /gene="trpD"
                     /locus_tag="OQN13_002516"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCS1615846.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14072..15433
                     /gene="trpCF"
                     /locus_tag="OQN13_002517"
     CDS             14072..15433
                     /gene="trpCF"
                     /locus_tag="OQN13_002517"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCS1615847.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15445..16638
                     /gene="trpB"
                     /locus_tag="OQN13_002518"
     CDS             15445..16638
                     /gene="trpB"
                     /locus_tag="OQN13_002518"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCS1615848.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            16638..17444
                     /gene="trpA"
                     /locus_tag="OQN13_002519"
     CDS             16638..17444
                     /gene="trpA"
                     /locus_tag="OQN13_002519"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCS1615849.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4168 a         4674 c         4408 g         4293 t
ORIGIN      
        1 cgtgagcatc aacacgcaca aggtcgggat cggcgaccgg atccaccgcc atgctggcaa
       61 tgacccctac ccccagccct aaccggacgt aagttttaat gacgtcagca tccgttgccg
      121 tgaagacgat acgcggcgtt aaccctgcgc ggttaaaggc agtatccagt tctgaacgtc
      181 cggtaaagcc gaaggtatat gtcaccaacg gatattgcgc cagttcttca atggtaatgg
      241 cttttttgcc tgccagcggg tgatccggag tgactacaat agcccgattc cagtggtagc
      301 acggtaacat cactaaatct tcatacagat gcagcgcttc tgtggcgata gtgaaatcag
      361 cattgccttt agagacggca tcagcaattt gtgtcggcga gccctggtgc atatgcaaag
      421 aaacgcgggg ataacgctca ataaagcctt tgataacgtt tggtaacgcg tagcgtgcct
      481 gggtatgcgt ggtggcgata tacagtgagc ctttatccgg ccaggtgtgc tctccggcaa
      541 ccgattttat ggcatcgact ttcgacagga cttcgcgagc gatacgaatt atttcttgcc
      601 ctgctggcgt tacctgcgtc aggtgcttgc cgcttcggga aaaaatttga atgcctagct
      661 cgtcttccag cattctgact tgtttactga tcccgggttg tgatgtgtaa agtccttccg
      721 ctgttgatga gacattcagg ttatgattga ccacctcaac aatatagcga agttgttgta
      781 atttcatgtt aaaccatcca cttagacgcc atcagaacat cgttttttcg tcacacctgg
      841 cgggtgacta atacgattta ataataaaaa aggtgctaac tataaccact atatcattta
      901 tagccttagt gtataggtgt gaagaaaaaa taatatgcaa taaaaaaggg ccgctttcgc
      961 gaccctttgt ttataaaaac ctgacagaat taaaggttat ttttttcctt caacccattt
     1021 gccatcaaca taaaatgctg accagccagt cgcctttccg tctttttccg aagagacata
     1081 ttgctgtttg gttttacggc taaagcgaac catggtctta ttaccttccg gatcctgctg
     1141 tggcgcatcg gccagataac gcagtttttc cggcagacgg tcgcggaagc gataaagctc
     1201 ttccaccagt ggcgcacgcg tttcacgcga tttcgggaaa gtgttggcag ccaggaaaac
     1261 acccgcagcg ccgtcacgca gcacgaaata agcatctgat ttttcgcacg gcagctcagg
     1321 taatggcacc ggatcttctt tcggtggtgc cacttcgccg ttacgtaaaa tcttacgtgt
     1381 gtttttacac tcttcgttgg tgcaggccat gtatttaccg aaacgcccca ttttcaggtg
     1441 catttcagag ccacattttt cacactcaac gatcgggccg tcataacctt taatgcggaa
     1501 ttcgccctct tcgatctcgt aaccgtcgca ggttgggtta ttaccacaga catgcaactt
     1561 acgtttcgga tcgatgagat agctgtccat cgccgtgccg catttcgggc aacgacgttt
     1621 tgcgcgcagc gcgttggttt cagcgtcttc gccttccagt acgttcagca cttcgttttc
     1681 cggcaccagg ttaatggtgg ttttgcaacg ctctttcggc ggcagcgcat aaccagaaca
     1741 gccaaggaat accccggtgc tcgctgtgcg aatccccatt ttgcgaccac aagtcgggca
     1801 gtcaatgctg gtcagaacca tctggttcgg gcgcatacca ccctcttccg gatctttttc
     1861 agctttatct aactgctggg tgaaatccga gaagaagtga tccagtacag ctttccactc
     1921 tgcttcgtga tttgccacca ggtcgaggct gttttccatc tgtgcggtga agtcgtagtt
     1981 cattaactcg cggaaatttt cttcaaggcg atcggtgacg atttcgccaa ttttttccgc
     2041 atagaaacga cgattttcta ctcgcacgta gccacgatcc tgaatggtcg aaatgatcga
     2101 cgcataggta gacggacgac cgataccgcg tttttccagc tctttaacca gcgatgcttc
     2161 actgaaacgg gctggcggct tggtaaagtg ctgggctggt gtaagttcaa cgagcgtcag
     2221 agcatcgcct ttattaactg ctggtaagat gcgatcttca tcgcctttac gcaacgcagg
     2281 catcactttt gtccagccat caaaacgcaa aatacgaccg cgtgctttca ggcggaaatc
     2341 gcccgcacca atggtcagcg tcgtggagtc atatttcgct ggggtcatct ggcaggctac
     2401 gaactgacgc cagattaact ggtacagttt ctgcgcatct gcttccatat ccttcagcga
     2461 ttccgccatc acattgacgt cagaaggacg aatcgcttcg tgcgcttcct gtgagttttc
     2521 tttgctggcg tactgattcg gactttccgg cagatatttc ttaccaaaat tatcgctgat
     2581 ataaccacga accatattta ccgcgtcctg actcaggtta gtggagtcgg tacgcatgta
     2641 agtgatatag cctgcttcat acaaacgctg cgccatcatc atggtttttt tcacgccaaa
     2701 tccaagacgg gtgctggcag cttgttgcag cgtagaggta ataaaaggag cgccaggttt
     2761 actggttgtc ggtttgtctt cacgttccag cacgctgtag cgcgcttttt ccagcagact
     2821 taccgcagcc tgagtttgtt ctttgttgac cggacggaac ggtttgtcgt tctgatgagt
     2881 cacctgtaac gccaacgctt caccagatgg cgtggtcgtg ctggcatcga cttcccagaa
     2941 ctcttccggc acgaacgctt taatttcacg ctcacgctcg accaccaggc gaaccgccac
     3001 cgactgcaca cgaccggcag acagaccacg agcgatcttt ttccatagca gcggcgaaac
     3061 catatacccc accacgcggt ccataaagcg acgcgcctgc tgggcattaa cacgatcaat
     3121 attcagctca cccggtttgt taaatgcctg gcggatcgcg tttttagtaa tttcgttaaa
     3181 caccactcgg ctatagcgcg catcatcacc cccaatcact tcccgcaggt gccatgcaat
     3241 ggcttcccct tcgcggtcaa ggtcggttgc gagatagatg tggtcggctt tttcagccag
     3301 ttgtttcagt tcagagacga ccttctcttt accaggcaac acttcatagt gcgcctccca
     3361 attgtgccac gggtcaaccc ccatacggtt gacgagagcg ccacgttcat cctttttagg
     3421 ctttttagcc gtcttggtgg aggtagagtc ggcactcttt ttggcagctg agccactggt
     3481 cggcaaatcg cggatgtgac cgacgctgga tttcaccacg tagtcactac ccagatactt
     3541 gttgatcgtt ttggcttttg ccggggactc aacgatgaca agagctttac ccatattcac
     3601 cttacctaat ttgattcttc caggaatgcg tcgcacgttg ataaaccttc cactggcaac
     3661 gagttaccga tatggactcg ttctctgcgg atatcaaccc cttgtcgtga ctctgttcac
     3721 gaaaattcag gtgactgagt taacaggtct tggcctacag gcgctatagc acgacggaat
     3781 tttggtcgaa tgtcaagcaa atctattgcc agattgacga aagcgtcaca ctgtacctga
     3841 taaaatccga tacgcaactt tattagcatg caaaatccaa agttgccagt aaggcgctct
     3901 tgccaccagc gtggtatatt tgtctgcaag ccatgcctgg cgtagactaa tgtccttgtt
     3961 taaggagtaa ataatgaaca aagagacaca acccatcgac cgcgaaacgc tgttgaaaga
     4021 ggccaacaaa atcattcgtg agcatgagga tacgctcgca gggattgaag ccaccggcgt
     4081 cacgcagcgt aatggcgtgc tggtatttat cggcgactac tttttagatg aacaaggatt
     4141 gccaacagcc aaaagcaccg cggtgtttaa tatgttcaaa cacctggccc atgtgctttc
     4201 agaaaaatat catctggttg attaaagcaa aacgcgaggt cttagcctcg cgtttgtctt
     4261 ttacatcaat ggcttttgac cccgctgcca ccagcgtagc aacaatcgat cggcgctctc
     4321 tgccgcgctg ccggtgaatc ggtcaatgag tcgtttacgc tgcatatagc gtacattaac
     4381 cacttcacgg ccttccatca ggctaagaat aacttcatca ctggtgttga tttcatcaac
     4441 caggcctttc tctaccgcct gttgtccgta ccagtgttca cccgttgcca cctgttcaat
     4501 atccagagac ggacgcatac gcttcacaaa atctttaaat aactgatgcg tttcgttcag
     4561 ctcttcgcgg aatttctccc gcccttcttc ggtattttca cccagcaagg tcagcgtgcg
     4621 cttatactgc ccggcggtgt gcagttcgat atcaatatct ttgcttttca ggaagcggtt
     4681 aaagttgggc atttgcgcca ccaccccaat ggaacccaca atagcaaacg gtgcggaaac
     4741 aattttgtcc gccacacagg ccatcatgta accgccgctg gcagcgactt tgtctaccgt
     4801 aacagttaaa ggaatgtttt tatcacgcag acgctgcagc tgcgaagccg ccaacccgta
     4861 accatgcacc atgccaccag ggctttccag acggagcaca acctgatcct gcggtttgaa
     4921 tgctgcgagt accgccgtta tctcttcacg tagcgagttc acttcatggg cgtccatgct
     4981 gcctttaaaa tccagcaccc agacgcgagg tttactgtca gttgccacct cgcccagttt
     5041 ggctttcgct tttgctgctt tcgcttcttg cttgtgcttt ttcttctgcg ctttgtgcca
     5101 ctgtttttgc tgatgtgagt ccatcagcgc cgcggccagt tcttctttca tctccttata
     5161 ctgttcgctg agattgttga cccgtaactc gccacgctgg cgtttattac gttgagcaac
     5221 attgacaata atggcggcaa tcgccgcaat cgctagcaca acggtaacga ttttcgccaa
     5281 aaacaaacca tattcagaca acaattccac gagtccccac cttggttaaa caacgctttg
     5341 gctcacagtt tacaacaggc atgttaagcc gtctcgcaga gatgttattg cagcgaaagt
     5401 atgacaaagt atccattttg cggtgttatc gccccaggct gattgaaatg cccttctgtt
     5461 tcaggcataa agccccaaag tcataaagta cactggcagc gcgacagaca acgtcgttga
     5521 ggagtagcca tgcattacca gccaaaacaa gatttactca atgatcgcat tatcctggtg
     5581 acgggagcca gcgatgggat tggtcgtgaa gccgcgatga cgtatgcacg ctatggtgcg
     5641 acagtgattc tgttgggccg taatgaagaa aaattacgtc aggtagccag ccacataaac
     5701 gaagaaactg ggcgtcagcc acagtggttt attctcgatt tgctgacctg cacgtccgaa
     5761 aattgccaac aactggcaca gcgcattgtc gttaattatc cgcgtctgga tggtgttttg
     5821 cataatgccg gattgctcgg cgatgtttgc ccaatgagcg aacaaaatcc gcaggtctgg
     5881 caggacgtca tgcaggtcaa cgttaatgcc acctttatgc tcacccaggc actgcttcct
     5941 ttattactca aatcggacgc cggttcactg gtctttactt catcaagcgt tgggcgtcag
     6001 ggacgagcca actggggtgc atatgcagcg tcgaaatttg ccaccgaagg gatgatgcag
     6061 gtactggccg atgaatatca gcagcgcctg cgcgtcaact gcattaaccc aggcggtacg
     6121 cgcaccgcaa tgcgcgcaag cgccttcccg accgaagatc cacagaaact taaaacaccc
     6181 gctgatatca tgccgctcta cctctggctg atgggcgatg acagccgccg taaaaccggc
     6241 atgacctttg acgcccaacc gggccgtaaa ccaggaattt cccaatgagt gatgaacgct
     6301 accaacagcg tcagcagcga gtgaaagaaa aagtagatgc tcgtgtggcc caggcccagg
     6361 atgaacgcgg tattatcatc gtctttaccg gcaatggaaa aggcaaaacc accgcggcat
     6421 ttggtacggc aacacgcgca gttggtcacg gaaaaaaagt gggcgtcgtg cagtttatta
     6481 aaggcacctg gcctaatggc gaacgcaatc tgctggagcc acatggcgtt gagtttcagg
     6541 tgatggcaac gggctttacc tgggatacac aaaaccgcga gtctgatacc gccgcctgcc
     6601 gcgaagtctg gcaacatgca aagcggatgc ttgctgattc ctcactggat atggttttgc
     6661 ttgatgaact gacgtatatg gtggcgtatg actatttgcc actggaagaa gtggtgcagg
     6721 cgttaaatga acgtccacat caacagacgg tgattatcac gggtcgaggc tgtcatcggg
     6781 atattcttga actggcggat acggtaagtg agctgcgccc catcaaacat gcgtttgatg
     6841 ccggtgttaa ggcgcaaata gggattgatt attaatctgt aaaaatgccg gatatgacgt
     6901 ccggcatact ttgattaacc gttattacgc ccgccagaac gacgaccgcc gctcacctga
     6961 ctgtgacgtt tcaccgcacg acgaatctga ttagccttca tgcgacgacg gtctttttct
     7021 acagcgactt tagagctggt ttctggcgga agctccacca gttcgcgcag atagttagtc
     7081 tgggcgaggt ccagctccgt ccagccaccg cgtggcagac cttttggcag tgggatatca
     7141 ccgtaacgaa cacggatcag gcggctcacc tgcacaccaa ccgcttccca cagacgacga
     7201 acttcacggt tacggccttc ggtcagagtc acgttgtacc actggttgat cccttcgccg
     7261 ccgctgaact tgatggtttt aaaagctgcc ggaccatctt ccagttgcac gccacgactc
     7321 aaatcacgca gtttcgcgtc gtcaacctga ccaaatacac gcacggcgta ttcacgttca
     7381 acttcacggc ttggatgcat taaacggttt gccagttcac catcggtggt gaacagcagc
     7441 agaccacagg tgttaacgtc cagacgacct acggcaatcc agcgtgcgcc acgcagcttt
     7501 ggcagacggt caaacaccgt cggacgccct tccggatcgt tacgggtaca caactcacct
     7561 tccggtttgt aataggccag aacgcgacaa atttgttcag cggactcacg taccgaaatc
     7621 agatgaccat cgatacgaat tttcagccca ggggtaactt caacgcgatc gccgagttta
     7681 gcaattttgc catcaacact cacacgaccg gcttcaataa tagattcgat ttcacggcgg
     7741 gagccgtggc cagcgcgggc cagcactttc tgtaattttt cgctcataga gcttcctttt
     7801 ggtgtcgcct tcccaggcgt ctgatatgct tttgtgcaac agtgagtcag aacttacatc
     7861 tgactcactg atagtaaagt ctgtttgaac tcttcaggcc ttaagtaaac gactcgacgt
     7921 tttaactggc ctggctttga ggcgcaacat actacactat ctttatggtt aaagcttgtt
     7981 tcgattaaca tcagcttaat taccagccac caccgcctcc gccacccgcg ccgccaccag
     8041 caccaccacc ggaataactt cgccccaaca aactggtttt ggcccccaga ctcaggtgca
     8101 tctgtaatga aaacaaaacg ccactacggg caatttccgg ggcgctaaca gccgctgtga
     8161 gtgatggggt gatacgtgct acccattctc ttccaaggtt tgctgagact gcccaaccta
     8221 gcaacgcgta atccagtttc cgtgtttccc ctttccgccg acgcccactg tgtgttgctg
     8281 cctctttacg cgcaagatag ttaacgatag cttcaccgtg ggcataacgt tgctgcccag
     8341 tttgtgtaaa ctttggcaga aaaatatacc ccatgccagt gagataaccg gtcaggaaaa
     8401 cagcgctaaa ataacccgct ggcatatacc agtaaaagaa tacatctccg acattgatgt
     8461 agagagaata gaaggccacg cctgaacaaa tcagtggtaa acaattcttc cgtcctcaag
     8521 gatgcggcca tttcgatatg tgtcgccctc ttctccagta aagagatcga tttcgctaaa
     8581 tggattaccc gcgatatcag ggaacttgag tgaaaatcgg gtcttataga tttcaaacgg
     8641 ccagtggtta ccagtcacgt tccagattaa cagatctgaa tctccctcac gcaggaaagc
     8701 atttttaacc tgataatgaa tttgataatg atagtcgcct tgaggcaaga aacgttgttt
     8761 atcgcccacg acaatactca ttaacccgat atgccagtca tgatgccagg gttcaggaat
     8821 accatcacgg gtgacgccaa caatatgata gttcagcagt gcagcgtccc caccaggctg
     8881 catccagagt cgtggtaaat cacgataaaa tccgtgttta atctgctgat ttcgcgcctg
     8941 aaaatgaata ttttctttga tgtcgagcac cccatccggt tgcagagtgg cttcaacatc
     9001 aaagagcgaa atcccttctt cataagaagg gatctccgtg ttttctgcat aacttagaga
     9061 tgatacaaat agccctgcta tcaacagcaa aatgcagcga aacttccctg ccatagtctt
     9121 ccatccgtgg aacgcaattt gttataagaa aggcttcaca tcacctacgc cttcacgcac
     9181 cacgaccggc gtatcatcgg taagatcaat aaccgttgtc ggtttctgcc cgagatagcc
     9241 gccgtgaata atcaaatcta cctgtttttc cagacggtct ttaatttctt cgggatcgga
     9301 ttcggtaaat tcgctgcctg gcagcattag cgaagtggaa agcatcggtt cgcccagtgc
     9361 ttcaagcaac gcctgcgcaa tcgggttaga aggcacacgc ataccgatgg ttttgcgttt
     9421 ttcctgcaac agacggcgtg gcacttcctt cgtccccttc aggatgaagg tatagttgcc
     9481 cggcgtgttg tttttcatta aacgaaacgc aacgttatca acaaatgaat aggtcgagag
     9541 ttcagaaaga tcgcgacaca tcagggtaaa gttgtgaccg tccggcagct ggcgaatacg
     9601 acaaatacgc tccatcgcgt ttttatcttc aattttacag ccgagcgcat agccggaatc
     9661 agttggataa acaatcaccc cgcctttacg cacgatctcc accgcctggt tgatcagacg
     9721 ttgctgtggg ttatcaggat gaatataaaa aaactggctc ataattccct ctctgtggtg
     9781 ttctgcggct gttcccatag ctgccagacg ccttcaacgc ctgcgggcag ccagagttta
     9841 cgacctagtt caatccacgg gcatggctga tgaaaatcag atccttgtga tgcccataaa
     9901 tgatgctgac gcgcaagggc cgccagctgg gtacgttcat tgggcgattg ctggcactgc
     9961 gcgacttcca tcgcgtcacc gtggtgttcg gcaaaatgcg caaccaatct tttcagccat
    10021 ttagcactaa gattgtaccg cccaggatga gctagcaccg ccttaccgcc agaatgatga
    10081 atgacatcaa tagcttgttc tattgtacac cactgtggcg gaacgtatcc ggttttcccg
    10141 cgcgccagat attttttaaa gacatccgcc attgaactgg ctttgccgca ccccacaagg
    10201 aaacgcgcaa aatgaccgcg cgtcaccgca ccgccctgcg ccagtcgtcg cgcgccttcc
    10261 agtgcccctg gtatttgcgc tttttcaagt ctttcggcaa tcagttgcgc ccgctgattg
    10321 cgccgttctg tctgctgcgc gaggaactca cacattagcg gatgagtaat atcaatattc
    10381 agcccgacaa tatgaatttc atgattttcc cagaccgtgg aaatttccac gccgggaata
    10441 agattcagcg ccagtcccga acgtgaaatt tcctctcttg ctggcacgat tgcagctgtg
    10501 ttgtcatggt cggtgatcgc cagggtaccg acgcgcatct cgactgcacg gtgcaccaat
    10561 acttctggcg tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact
    10621 gcgtaattcg tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat
    10681 cataacagtt ctggcaaata ttctgaaatc agctgttgac aattaatcat cgaactagtt
    10741 aactagtgcg caagttcacg taaaaagggt atcgacaatg aaagcaattt tcgtactgaa
    10801 agcttggtgg cgcacttcct gaaacgggca gtgtattcac catgcgtaaa gcaatcagat
    10861 acccagcccg cctaatgagc gggctttttt ttgaacaaaa ttagagaata acaatgcaaa
    10921 cacaaaaacc gactctcgaa ctgctaacct gcgaaggcgc ttatcgcgac aacccgactg
    10981 cgctttttca ccagttgtgt gggaatcgtc cggcaacgct gctgctggaa tccgcagata
    11041 tcgacagcaa agatgattta aaaagcctgc tactggtaga cagtgcgctg cgcattacag
    11101 cattaggtga cactgtcaca attcaggcac tttccggcaa cggcgaagcc ctgctggcac
    11161 tactggataa cgccctgcct gcgggtgtgg aaaatgaaca atcaccaaac tgccgtgtgc
    11221 tgcgcttccc ccctgtcagt ccactgctgg atgaagacgc ccgcttatgc tccctttcgg
    11281 tttttgacgc tttccgttta ttgcagaatc tgttgaatgt accgaaggaa gaacgagaag
    11341 caatgttctt cggcggcctg ttctcttatg accttgtggc gggatttgaa gatttaccgc
    11401 aactgtcagc ggaaaataac tgccctgatt tctgttttta tctcgctgaa acgctgatgg
    11461 tgattgacca tcagaaaaaa aacacccgca ttcaggccag cctgtttgct ccgaatgaag
    11521 aagaaaaaca acgtctcact gctcgcctga acgatcttcg ccagcagctg accgaagccg
    11581 cgccgccgct gccggtggtt tccgtgccgc atatgcgttg tgaatgtaat cagagcgatg
    11641 aagagttcgg tggcgtagtg cgtttgttgc aaaaagcgat tcgcgctgga gaaattttcc
    11701 aggtggtgcc atctcgccgt ttctctctgc cctgcccgtc accgctggcg gcctattacg
    11761 tgctgaaaaa gagtaatccc agcccgtaca tgttttttat gcaggataat gatttcaccc
    11821 tgtttggcgc gtcgccggaa agttcgctca agtatgacgc caccagccgc cagattgaga
    11881 tctacccgat tgccggaaca cgtccacgcg gtcgtcgtgc cgatggctcg ctggacagag
    11941 acctcgacag ccgcatcgaa ctggaaatgc gtaccgatca taaagagctt tctgaacatc
    12001 tgatgctggt ggatctcgcc cgtaatgatc tggcacgcat ttgcaccccc ggcagcagct
    12061 acgtcgccga tcttaccaaa gttgaccgtt actcttacgt gatgcacctg gtctcccgcg
    12121 tggtcggtga gctgcgccac gatctcgacg ccctgcacgc ttaccgcgcc tgtatgaata
    12181 tggggacgtt aagcggtgcg ccgaaagtac gcgccatgca gttaattgcc gaggcggaag
    12241 gtcgtcgccg cggcagctac ggcggcgcgg taggttattt caccgcgcat ggcgatctcg
    12301 acacctgcat tgtgatccgc tcggcgctgg tggaaaacgg tatcgccacc gtgcaagccg
    12361 gtgctggcgt agtccttgat tctattccgc agtcggaagc cgacgaaacc cgtaataaag
    12421 cccgcgctgt actgcgcgct attgccaccg cgcatcatgc acaggagact ttctgatggc
    12481 tgacattctg ctgctcgata atatcgactc ttttacgtac aacctggcag atcagttgcg
    12541 cagcaatggg cataatgtgg tgatttaccg caaccatatt ccggcgcaga ccttaattga
    12601 acgcctggcg acgatgagca atccgatact gatgctttct cctggccccg gtgtgccgag
    12661 cgaagccggt tgtatgccgg aactcctcac ccgcttgcgt ggcaagctgc ccattattgg
    12721 catttgcctc ggacatcagg caattgtcga agcttacggg ggctatgtcg gtcaggcggg
    12781 cgaaattctt cacggtaaag cgtcgagcat tgaacatgac ggtcaggcga tgtttgccgg
    12841 attaacaaac ccgctgccgg tggcgcgtta tcactcgctg gttggcagta acattccggc
    12901 cggtttaacc atcaacgccc attttaatgg catggtgatg gcagtacgtc acgatgcgga
    12961 tcgcgtttgt ggattccagt tccatccgga atccattctc accacccagg gcgctcgcct
    13021 gctggaacaa acgctggcct gggcgcagca gaaactagag ccagccaaca cgctgcaacc
    13081 gattctggaa aaactgtatc aggcgcagac gcttagccaa caagaaagcc accagctgtt
    13141 ttcagcggtg gtgcgtggcg agctgaagcc ggaacaactg gcggcggcgc tggtgagcat
    13201 gaaaattcgc ggtgagcacc cgaacgagat cgccggggca gcaaccgcgc tactggaaaa
    13261 cgcagcgccg ttcccgcgcc cagattatct gtttgctgat atcgtcggta ctggcggtga
    13321 cggcagcaac agtatcaata tttctaccgc cagtgcgttt gtcgccgctg cctgtgggct
    13381 gaaagtggcg aaacacggca accgtagcgt ctccagtaaa tctggttcgt ccgatctgct
    13441 ggcggcgttc ggtattaatc ttgatatgaa cgccgataaa tcgcgccagg cgctggatga
    13501 gttaggtgtt tgtttcctct ttgcgccgaa gtatcacacc ggattccgcc acgcgatgcc
    13561 ggttcgccag caactgaaaa cccgcaccct gttcaatgtg ctggggccat tgattaaccc
    13621 ggcgcatccg ccgctggcgt taattggtgt ttatagtccg gaactggtgc tgccgattgc
    13681 cgaaaccttg cgcgtgctgg ggtatcaacg cgcggcggtg gtgcacagcg gcgggatgga
    13741 tgaagtttca ttacacgcgc cgacaatcgt tgccgagctg catgacggcg aaattaagag
    13801 ctatcaattg accgctgaag attttggcct gactccctac caccaggagc aactggcagg
    13861 cggaacaccg gaagaaaacc gtgacatttt aacacgcttg ttacaaggta aaggcgacgc
    13921 cgcccatgaa gcagccgtcg ctgcgaacgt cgccatgtta atgcgcctgc atggccatga
    13981 agatctgcaa gccaatgcgc aaaccgttct tgaggtactg cgcagtggtt ccgcttacga
    14041 cagagttacc gcgctggcgg cacgagggta aatgatgcaa accgttttag cgaaaatcgt
    14101 cgcagacaag gcgatttggg tagaaacccg caaacagcag caaccgctgg ccagttttca
    14161 gaatgaggtt cagccgagca cgcgacattt ttatgatgcg ctacagggtg cacgcacggc
    14221 gtttattctg gagtgcaaga aagcgtcgcc gtcaaaaggc gtgatccgtg atgatttcga
    14281 tccggcacgc attgcctcca tttataaaca ttacgcttcg gcaatttcgg tgctgactga
    14341 tgagaaatat tttcagggga gctttgattt cctccccatc gtcagccaaa tcgccccgca
    14401 gccgatttta tgtaaagact tcattatcga cccttaccag atctatctgg cgcgctatta
    14461 ccaggccgat gcctgcttat taatgctttc agtactggat gacgaccaat atcgccagct
    14521 tgcagccgtc gctcacagtc tggagatggg ggtgctgacc gaagtcagta atgaagagga
    14581 actggagcgc gccattgcat tgggggcaaa ggtcgttggc atcaacaacc gcgatctgcg
    14641 tgatttgtcg attgatctca accgtacccg cgagcttgcg ccgaaactgg ggcacaacgt
    14701 gacggtaatc agcgaatccg gcatcaatac ttacgctcag gtgcgcgagt taagccactt
    14761 cgctaacggt tttctgattg gttcggcgtt gatggcccat gacgatttgc acgccgccgt
    14821 gcgccgggtg ttgctgggtg agaataaagt atgtggtctg acgcgtgggc aagatgctaa
    14881 agcagcttat gacgcgggcg cgatttacgg tgggttgatt ttggttgcga catcaccgcg
    14941 ttgcgtcaac gttgaacagg cgcaggaagt gatggctgcg gcaccgttgc agtatgttgg
    15001 cgtgttccgc aatcacgata ttgccgatgt ggtggacaaa gctaaggtgt tatcgctggc
    15061 ggcagtgcaa ctgcatggta atgaagatca gctgtatatc gatacgctgc gtgaagctct
    15121 gccagcacac gttgccatct ggaaagcatt aagcgtcggt gaaaccctgc ccgcccgcga
    15181 gcttcagcac gttgataaat atgttttaga caacggccag ggtggaagcg ggcaacgttt
    15241 cgactggtca ctattaaatg gtcaatcgct tggcaacgtt ctgctggcgg ggggcttagg
    15301 cgcagataac tgcgtggaag cggcacaaac cggctgcgcc ggacttgatt ttaattctgc
    15361 tgtagagtcg caaccgggca tcaaagacgc acgtcttttg gcctcggttt tccagacgct
    15421 gcgcgcatat taaggaaagg aacaatgaca acattactta acccctattt tggtgagttt
    15481 ggcggcatgt acgtgccaca aatcctgatg cctgctctgc gccagctgga agaagctttt
    15541 gtcagcgcgc aaaaagatcc tgaatttcag gcccagttca acgacctact gaaaaactat
    15601 gccgggcgtc caaccgcact gaccaaatgc cagaacatta cggctggcac gaacaccacg
    15661 ctgtatctga agcgcgaaga tttgctgcac ggcggcgcgc ataaaactaa ccaggtgctc
    15721 ggtcaggctt tactggcgaa gcggatgggt aaaaccgaaa tcatcgccga aaccggtgcc
    15781 ggtcagcatg gcgtggcgtc ggcccttgcc agcgccctgc tcggcctgaa atgccgtatt
    15841 tatatgggtg ccaaagacgt tgaacgccag tcgcctaacg tttttcgtat gcgcttaatg
    15901 ggtgcggaag tgatcccggt gcatagcggt tccgcgacgc tgaaagatgc ctgtaacgag
    15961 gcgctgcgcg actggtccgg tagttacgaa accgcgcact atatgctggg caccgcagct
    16021 ggcccgcatc cttatccgac cattgtgcgt gagtttcagc ggatgattgg cgaagaaacc
    16081 aaagcgcaga ttctggaaag agaaggtcgc ctgccggatg ccgttatcgc ctgtgttggc
    16141 ggcggttcga atgccatcgg catgtttgct gatttcatca atgaaaccaa cgtcggcctg
    16201 attggtgtgg agccaggtgg tcacggtatc gaaactggcg agcacggcgc accgctaaaa
    16261 catggtcgcg tgggtatcta tttcggtatg aaagcgccga tgatgcaaac cgaagacggg
    16321 cagattgaag aatcttactc catctccgcc ggattggatt tcccgtctgt cggcccacaa
    16381 cacgcgtatc ttaacagcac tggacgcgct gattacgtgt ctattaccga tgatgaagcc
    16441 cttgaagcct tcaagacgct gtgcctacac gaagggatca tcccggcgct ggaatcctcc
    16501 cacgccctgg cccatgcgct gaaaatgatg cgcgaaaacc cggaaaaaga gcagctactg
    16561 gtggttaacc tttccggtcg cggcgataaa gacatcttca ccgttcacga tattttgaaa
    16621 gcacgagggg aaatctgatg gaacgctacg aatctctgtt tgcccagttg aaggagcgca
    16681 aagaaggcgc attcgttcct ttcgtcacgc tcggggatcc gggcattgag cagtcattga
    16741 aaattatcga tacgctaatt gaagccggtg ctgacgcgct ggagttaggc atccccttct
    16801 ccgacccact ggcagatggc ccgacgattc aaaacgccac tctgcgcgcc tttgcggcag
    16861 gtgtgactcc ggcacaatgt tttgaaatgc tggcactgat tcgccagaaa cacccgacca
    16921 ttcccattgg cctgttgatg tatgccaatc tggtgtttaa caaaggcatt gatgagtttt
    16981 atgcccagtg cgagaaagtc ggcgtcgatt cggtgctggt tgccgatgtg ccaattgaag
    17041 agtccgcgcc cttccgccag gccgcgttgc gccataatgt cgcacctatc ttcatctgcc
    17101 cgccaaatgc cgatgacgac ctgctgcgcc agatagcctc ttacggtcgt ggttacacct
    17161 atttgctgtc acgagcgggc gtgaccggcg cagaaaaccg cgccgcgtta cccctcaatc
    17221 atctggttgc gaagctgaaa gagtacaacg ctgcacctcc attgcagggg tttggtattt
    17281 ccaccccgga tcaggtaaaa gcagcgattg atgcaggagc tgcgggcgcg atttctggtt
    17341 cagccattgt taaaatcatc gagcaacata ttaatgagcc agagaaaatg ctggcggcac
    17401 tgaaagtttt tgtacaaccg atgaaagtgg cgacgcgcag ttaatccccc tgccgccagt
    17461 tccgctggcg gcattttaac tttctttaac gaagccggaa aaatcctaaa ttcatttaat
    17521 atttatcttt ttaccgtttc gct
//
LOCUS       DAJVRF010000082        17364 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJVRF010000082 DAJVRF010000000
VERSION     DAJVRF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.082"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..418)
                     /locus_tag="OQN13_002520"
     CDS             complement(<1..418)
                     /locus_tag="OQN13_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCS1615850.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAE"
     gene            complement(604..688)
                     /locus_tag="OQN13_002521"
     tRNA            complement(604..688)
                     /locus_tag="OQN13_002521"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(652..654),aa:Leu,seq:caa)
     gene            884..1903
                     /gene="ahr"
                     /locus_tag="OQN13_002522"
     CDS             884..1903
                     /gene="ahr"
                     /locus_tag="OQN13_002522"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCS1615851.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1907..2470)
                     /gene="idnK"
                     /locus_tag="OQN13_002523"
     CDS             complement(1907..2470)
                     /gene="idnK"
                     /locus_tag="OQN13_002523"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCS1615852.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2687..3718
                     /gene="idnD"
                     /locus_tag="OQN13_002524"
     CDS             2687..3718
                     /gene="idnD"
                     /locus_tag="OQN13_002524"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HCS1615853.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3742..4506
                     /gene="idnO"
                     /locus_tag="OQN13_002525"
     CDS             3742..4506
                     /gene="idnO"
                     /locus_tag="OQN13_002525"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCS1615854.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4571..5890
                     /gene="idnT"
                     /locus_tag="OQN13_002526"
     CDS             4571..5890
                     /gene="idnT"
                     /locus_tag="OQN13_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HCS1615855.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            5957..6955
                     /gene="idnR"
                     /locus_tag="OQN13_002527"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             5957..6955
                     /gene="idnR"
                     /locus_tag="OQN13_002527"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HCS1615856.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7033..8535
                     /locus_tag="OQN13_002528"
     CDS             7033..8535
                     /locus_tag="OQN13_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCS1615857.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8654..9736)
                     /gene="lptG"
                     /locus_tag="OQN13_002529"
     CDS             complement(8654..9736)
                     /gene="lptG"
                     /locus_tag="OQN13_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCS1615858.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9736..10836)
                     /gene="lptF"
                     /locus_tag="OQN13_002530"
     CDS             complement(9736..10836)
                     /gene="lptF"
                     /locus_tag="OQN13_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCS1615859.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            11103..12614
                     /gene="pepA"
                     /locus_tag="OQN13_002531"
     CDS             11103..12614
                     /gene="pepA"
                     /locus_tag="OQN13_002531"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCS1615860.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12748..13191
                     /gene="holC"
                     /locus_tag="OQN13_002532"
     CDS             12748..13191
                     /gene="holC"
                     /locus_tag="OQN13_002532"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCS1615861.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13191..16046
                     /gene="valS"
                     /locus_tag="OQN13_002533"
     CDS             13191..16046
                     /gene="valS"
                     /locus_tag="OQN13_002533"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCS1615862.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16102..17298)
                     /locus_tag="OQN13_002534"
     CDS             complement(16102..17298)
                     /locus_tag="OQN13_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCS1615863.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDATYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4460 a         4319 c         4435 g         4150 t
ORIGIN      
        1 actcagcgat attacgttca ccaagcgctg caaaaagatt atcttccaga ctttttagca
       61 cacgatgggc atgatcttca gaccactttt tattggtgcc atgccactca atcgcgactt
      121 ctttaaaggt tcgtgcttta ctctgttcaa ccttatcact tttctttttg tctcccggat
      181 cgacgccatt cgcaagcagc ttacgtggct cgtcacgacg tattctggca tccgcgagtg
      241 tgatttcagg ataaacccca agtgccagca tcttttgctt tccctcataa cggtactgca
      301 aacgccagta cttagaacca tttggatgga caagcagatg cataccgcca ccatcagtca
      361 acttatattg cttatctgtt ggcttagctg ttctgacttt gatgtctgtt aatgccatgc
      421 gtattccctc cctgttggta caagcattat cgaaccggaa ataccatcat ctgtaccaac
      481 atatgttggt agaagtacgt tgatatcggt tgaccttgag agagttaata tagcgggaaa
      541 ggtagataaa tactgaattt caggcataaa aaagacctca gttgaggtct attgacatac
      601 ttttggtgcc gaaggccgga ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa
      661 ctgcgtctac cgatttcgcc acttcggcac tgaagaggta tgcggaaaac gttgtggatt
      721 atacctgtcg cacgccacca tgcaagcgcc agcttgctcg cacctcgcta agtgctgaaa
      781 ttttcagcat tgcatacagc gatgtgtaac ctttgtcaca ctccaggcac cccgccctgc
      841 catgctctac acttcccaga caacaccaga gaaggaccaa aaaatgtcga tgataaaaag
      901 ctatgccgca aaagaagcgg gcggcgaact ggaagtttat gagtacgagc ccggtgaatt
      961 gaagccacaa gatgttgaag tgcaggtgga ttactgcggg atctgccatt ccgatctgtc
     1021 gatgatcgac aacgaatggg gattttcaca gtatccgctg gttgccgggc atgaggtgat
     1081 tgggcgcgtg gtggcgctcg ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg
     1141 tgtcgggatt ggctggacgg cgcgtagctg cgggcattgc gacgcctgta ttagcggtaa
     1201 tcagatcaac tgcgagcaag gtgcggtgcc aacaattatg aatcgcggcg gttttgccga
     1261 gaagttgcgt gcagactggc aatgggtgat cccactgccg gaaaatatcg atatcgagtc
     1321 cgccgggccg ctgctgtgtg gtggtatcac ggtctttaaa ccactgttga tgcaccatat
     1381 cactgctacc agccgcgttg gggtaattgg tattggcggg ctggggcata tcgctataaa
     1441 acttctgcac gcaatgggat gcgaggtgac agcctttagt tcgaatcctg cgaaagagca
     1501 ggaagtactg gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa
     1561 aacacttgcg gggcagttcg atctcattat caacaccgtc aacgtcagcc tcgactggca
     1621 gccttatttt gaggcgctga cgtatggcgg taatttccac accgtcggtg cggtcctcac
     1681 gccgctgtct gttccggcct ttacgttaat tgcgggcgac cgcagcgtct ctggttcagc
     1741 aaccggcaca ccttatgagc ttcgtaagct gatgcgcttt gcggcccgca gcaaggttgc
     1801 gccgaccacc gaactgttcc cgatgtcgaa aataaacgac gccatccagc atgtgcgcaa
     1861 cggtaaggcg cgttaccgcg tggttctgaa agccgatttt tgaaaatcat tcgcagcgct
     1921 gatctgaggc gctaccccct ttcgcacata ttctgtcttg tcgtatcgcc agcacggcct
     1981 gccgacattg ttcggtgaca ttggcaatat catggttgat atcaatgcgt acaatatcct
     2041 gttcatctgc ttgtggacgc tccagcgcct caaactgact ttttagtaac gctaccggca
     2101 taaaatgccc agcccgccgc tgcattcgcg cgagaatagt ttcatagtcg ccatctaacc
     2161 agaggaaatg aacatggggg ctacccttgc gtaaaatatc acgatactgt ttttttaatg
     2221 atgaacagac aataaatcct gtttcattct ttttataaag actgtatgaa gcatcattta
     2281 agcgttccag ccagggaagt cgatcttcat cagataatgg aataccctgc gacattttat
     2341 ctatattttt ggctggatga agatcgtcac catcaataaa tttagcagat aataacgcgg
     2401 caaccttgct accaattaat gttttaccac tccctgaaac gcccatcaaa ataaagcttt
     2461 cacccgccat ttaaaatctc ctgaatcatc ttacctgctg cgatactacg cctggtggca
     2521 taataatttc acgttttttt atttctgctg ataagaatta caaggcacat cacgttatgc
     2581 gtaacatagt aatgtaacaa ttttctgacg tgatcttcat cacaaataat gacaattaaa
     2641 accgcttaaa tgcttccgag gtgtctacct gaccagtgag gaatttatgc aagtgaaaac
     2701 acagtcctgc gttgttgcgg acaagaaaac tgttgccgtt accgagcaga cgatagattg
     2761 gaataataat ggaacattag tacaaataac ccgaggtgga atttgcggtt ccgatttaca
     2821 ttattatcag gaaggaaaag taggtaattt catgataaag gcaccgatgg tgttaggtca
     2881 tgaagttatc ggtaaagtta ttcatagcga ctcatcagaa ttacatgaag ggcaaacggt
     2941 agccattaat ccgtctaaac cgtgcggtca ctgcaaatac tgcattgaac ataacgagaa
     3001 tcagtgtaca gatatgcgtt tttttggcag tgccatgtat ttccctcatg ttgatggtgg
     3061 ttttacccgt tataaaatgg tcgaaacgtc gcaatgtgtc ccttatccgg ccaaagctga
     3121 cgaaaaggtt atggcttttg ccgaaccttt agccgtcgcg attcatgccg cacatcaggc
     3181 cgacgagtta cagggcaagc gagtatttgt ttccggtgtt gggcccattg gctgcctgat
     3241 tgtcagtgct gtgaaaacac tggggtccgc ggaaattgtc tgtgccgatg tgagtccccg
     3301 ttccctttcg ctggccaaag agatgggtgc ggatgtgctc gtaaacccac aaaacgacga
     3361 catggatcac tggaaagcgg aaaaaggcca tttcgatgtc agctttgaag tgtccggtca
     3421 tccttcatca gtgaatacct gtctggaggt cactcgtgca cgcggcgtaa tggtgcaggt
     3481 agggatggga ggcgcgatgg cagaattccc aatgatgacg ttgattggta aggagatttc
     3541 actcaaaggc tctttccgtt ttaccagcga atttaatacc gcagtgtcat ggctggcgaa
     3601 tggcgttatc aatccactgc ctttactgag tgctgaatat ctcttcactg acctggaaga
     3661 ggcgctacgt ttcgccggtg ataaaaccca ggcagcaaaa gtccagcttg ttttctaaga
     3721 gataaataaa ggaataatac aatgaacgat ctattttcac tggcaggaaa aaatatcttg
     3781 attaccggtt cagcacaggg cattggctat ttactggcaa ccggcctggg taaatatggc
     3841 gcacaaataa ttattaatga tattactgcc gaacgcgcag aacttgctgt agaaaaactc
     3901 caccaggagg gtattcaggc cgttgccgca ccttttaatg ttactcataa acatgaaatt
     3961 gatgccgccg ttgaacatat cgaaaaggac atcggcccca ttgatgtgct ggtgaataac
     4021 gccggtatcc agcgccgtca tccttttact gagttccctg aacaagagtg gaatgatgtg
     4081 atcgcagtaa accagaccgc cgtgttcctg gtatcgcaag cggtaactcg tcacatggtt
     4141 gaacgcaagg caggtaaagt tattaatatt tgctcgatgc aaagcgaact gggacgtgac
     4201 accatcaccc cttatgccgc atcgaaaggg gcggtaaaaa tgctcacccg cggcatgtgc
     4261 gtcgagctgg cgcgccacaa tattcaggtc aacggtattg cgccgggcta tttcaaaaca
     4321 gaaatgacta aagcactggt tgaggacgaa gccttcaccg cctggttgtg caaacggacc
     4381 cccgccgcac gctggggaga tccgcaggaa ctgattggtg ctgcggtgtt cctttcttca
     4441 aaagcctctg atttcgtaaa cggccacctg ttgtttgttg atggcggcat gttagtggct
     4501 gtttaacagc caccacccta cgtgcagcgc gaaagcgctg cttttctggc actaataaga
     4561 gataaagact atgccattaa tcattattgc ggcaggcgtc gcgctgcttc ttatcctgat
     4621 gatcggcttt aaagttaacg gctttattgc cctcgttctg gtagctgccg tcgtcggatt
     4681 tgccgaaggg atggatgcac aggccgtcct gcactctata caaaatggta tcggcagcac
     4741 gctcggcggg ctggcaatga tcctcggttt cggggccatg ttaggcaagc tgatttctga
     4801 tacgggcgcg gcacaacgta tcgccactac gctgattgct acttttggta aaaaacgcgt
     4861 gcaatgggcg ctagtgatca ccggtctggt tgtgggcctc gccatgtttt ttgaagtggg
     4921 ttttgtcctg ctgttgccgt tggtatttac catcgtggca tcatctggat tacccctgtt
     4981 gtatgttggc gtaccgatgg tagcagcgct ctctgtaacc cactgttttc tgccgccaca
     5041 tccagggcct actgccatcg cgactatctt tgaggctaat ctcggaacga ctttactgta
     5101 tggatttatc attaccattc cgacagttat tgtcgcagga ccgctgtttt ctaaactgct
     5161 aactcgcttt gagaaagcac caccggaagg cttatttaat cctcatctgt ttagcgaaga
     5221 ggagatgccc tccttctgga acagtatttt tgctgcagtg atcccggtca tcctgatggc
     5281 tatcgccgcc gtttgtgaaa ttacgttacc gaaaactaac accgtgcgcc tcttctttga
     5341 atttgtcggt aaccctgccg ttgcgctgtt tattgccatt gttattgcga ttttcacact
     5401 gggccgacgt aatggacgca ccatcgagca aatcatggat atcattgggg attctatagg
     5461 cgctatcgcg atgattgtgt ttattatcgc tggcggcggc gcgtttaagc aggtattagt
     5521 agatagtggt gtcgggcagt atatttcaca cttaatgacc ggaactacgc tttcgccgtt
     5581 attgatgtgc tggactgtcg cggcgctgtt gcgtatcgct ctgggctctg ccaccgtcgc
     5641 ggccattacc accgcgggtg tggtgttgcc gattatcaac gttacccatg ccgatcccgc
     5701 tttaatggta ctggcaactg gtgcgggcag cgtgatcgcg tcacacgtaa acgaccctgg
     5761 cttctggcta tttaaagggt attttaatct gacggttggt gaaacgttgc gtacctggac
     5821 agtgatggaa acccttattt ctattatggg tttgctgggc gtgttagcca ttaacgccgt
     5881 attgcactga aagaataaaa tattgtgatg tagtttcggg ggaagattga agattccccc
     5941 gacgttggga gaagttatgc gcaatcacag aatttcttta caggatatcg ctacgctggc
     6001 tggcgtaaca aaaatgaccg tgagtcgtta tatccgctcg ccgaaaaagg tggcaaagga
     6061 aacaggcgag cgcatcgcca aaattatgga agagatcaat tatatcccta atcgcgctcc
     6121 cggcatgttg ttgaatgcac aaagttatac cctcggtatc ttgattccgt catttcaaaa
     6181 tcaactcttc gctgacattc tcgcgggaat agaatccgtc acctctgtgc ataattatca
     6241 gacccttatt gctaattaca attacgatcg cgattccgaa gaagagtcgg tgattaacct
     6301 gctctcttat aacattgacg ggattattct ttcggaaaaa tatcacacca tcaggacggt
     6361 gaaatttctg cgttcagcca ccattccggt cgtggaattg atggatgtac agggagaacg
     6421 gctggatatg gaggtcggtt ttgataatcg gcaggctgct tttgacatgg tgtgtaccat
     6481 gctggataag cgagtcaggc gcaaaattct gtatctcggt tccaaagatg acacccgcga
     6541 tgaacagcgt tatcaggggt attgcgatgc gatgatgctg cataatcttt ccccattacg
     6601 catgaatcca cgcgccatct catccattca tttaggaatg cagttaatgc gtgatgccct
     6661 tagcgcaaac ccggatttag acggtgtatt ttgcaccaat gacgatattg cgatgggtgc
     6721 gcttctgctg tgccgcgagc gaaacctcgc cgtgccagaa caaatttcca ttgcgggttt
     6781 tcatgggctg gagattggca ggcaaatgat cccaagcctc gccagcgtaa ttactccccg
     6841 ttttgatatt ggtcggatgg ccgcccaaat gttgctgagc aagattaaga ataacgatca
     6901 caaccacaac actgttgacc tcgggtatca gatttatcac ggtaacaccc tttaggttat
     6961 tttctcttca ttacacatat tctctttgcc ggacgttgcc tatactccag gcaggaaact
     7021 ggaggaaatc tcatgagcga acccctgtta attgcccgca cgccggacac agaactgttt
     7081 ttactgccgg gaatggctaa ccgtcacggg ctgattactg gcgcaacggg gacgggtaaa
     7141 actgtcacgt tgcaaaaact ggcagagtca ttgtcggaaa tcggtgttcc ggtgtttatg
     7201 gccgatgtga aaggcgatct gaccggcatc gcgcaggcag gaacggcgtc ggaaaaactg
     7261 ctcgcaagac tgaaaaatat cggcgtcaac gactggcaac cgcatgccaa tccggtggtg
     7321 gtgtgggata tctttggcga gaaaggccat ccggtgcggg cgacggtttc ggatctgggg
     7381 ccgctgttgc tggcacgact gttgaatctc aacgatgtgc aatctggcgt gctgaatatt
     7441 atcttccgca ttgcggacga tcagggattg ttgctgctcg actttaaaga tttgcgggcg
     7501 attacccagt acatcggcga taacgccaaa tccttccaga atcagtacgg aaatatcagt
     7561 agcgcatcgg ttggtgccat ccagcgcgga ttactgtcgc tggaacagca aggcgcagaa
     7621 cacttctttg gtgagccgat gctggatatc aaagactgga tgcgcaccga tgccaacggt
     7681 aaaggcgtta tcaatatcct cagcgccgag aaactttatc agatgccgaa actgtacgcc
     7741 gccagcctgc tgtggatgct ttcagagtta tatgaacaat tgcctgaagc gggcgatctg
     7801 gagaaaccaa aactggtgtt tttcttcgac gaagcacatc tgctgtttaa cgatgcgcca
     7861 caggtactgc tggataagat tgagcaggtg ataaggctta ttcgctcaaa aggcgtgggc
     7921 gtctggttcg tttcgcaaaa cccgtcagat atcccggata acgtgctagg gcagctcggt
     7981 aatcgcgttc aacacgcttt gcgtgctttt acgcctaaag atcagaaagc agtgaaggca
     8041 gcggcgcaaa ccatgcgggc caatccggcg tttgataccg aaaaggcaat ccaggaactg
     8101 gggaccggcg aggcgttaat ctcgtttctc gatgcgaaag gaagtccttc agtggtggaa
     8161 cgggcgatgg tgatcgcacc ttgttcgcga atggggccgg tgacggaaga tgagcgtaat
     8221 ggcctgatta atcactcccc ggtgtatggc aaatatgaag atgaggtgga ccgcgagtcc
     8281 gcctatgaga tgctgcaaaa aggctttcag gccagtaccg agcagcaaaa taatcccccc
     8341 gtgaaaggga aagaggtggc ggtggatgac ggtattcttg gtggattgaa ggatattttg
     8401 tttggcacta ccggaccacg cggcgggaag aaagatggtg tggtgcaaac aatggcgaaa
     8461 agcgccgctc gccaggtgac gaatcagatt gtacgcggga tgttggggag tttgctgggg
     8521 gggagaaaaa ggtaagtcag acgtgatctg tgcccctcac cctaaccctc tccccaaagg
     8581 ggcgagggga ccgttcgaac tcgtatttga ctttgtcaac aataataaag ggagctttcg
     8641 ctcccttgtt agattacgat tttctcatta acagccacag gctgattaag aagaagctgg
     8701 cgcttggcaa cagtgcgccg atgatcggcg ggatgccata aaccaacgtc agcgggccga
     8761 agatctggtc cagtacgtag aagacaaaac cgaagctgat accggtgacc acacgcacgc
     8821 ccatcggtac gctacgcagt gggccaaaga tgaacgacag cgccatcaac atcatcaccg
     8881 ccacggatag cggctggaag attttgctcc acatgttgag ctgataacgt ccggcatcct
     8941 gaccgctcga cttcagatac ttcacatagt tgtgcaaacc actgatggag agtgcatccg
     9001 ggtccagcgc caccacgccc agtttgtctg gcgtgaggtt ggttttccag gtgccgctca
     9061 ccgtctgcga accggtgatc tgtttcggat tggtcagatc agattcatca acctgcgaca
     9121 gacgccagac tttatgttcc gggtcaaact tcgcggtagc ggcataacgt acggattgca
     9181 gacgacgatt ctcgttaaag gcataaatac tgatgccacc taactcttcg tcacctttaa
     9241 cccgctcaat gtagacgaag ttgttgccat ctttcgccca taagccttgc tgagtggaga
     9301 gcaacgagcc gccgtacatc gcctgcgcac ggtagttacg cgccatctgc tcgccctgcg
     9361 acgcgaccca ttcaccaatc gccatcgtca gcaagaccag cggaatggcg gttttcatca
     9421 ccgacagcgc cacctgcata cgggtaaaac cagaagcctg catcaccacc agttcgctgc
     9481 gctgcgccag catcccaaga ccaagcaacg ccccaagcag agccgccatc gggaagaaga
     9541 tttgcacatc tttcggcacg ctcagcaagg tatacattcc tgcgcctaac gcgtcgtaac
     9601 tcccctgccc ggcttttttc agctgatcga caaacttgat aatgcccgac agcgacacca
     9661 gcatgaacag cgtcatcatg atggtggtga aaatagtttt accgatatag cggtcaagta
     9721 cgccaaaagg ttgcatcaca ccgctccttt acgcgaaaaa ctggcgcgca ggcggcggac
     9781 cggcacggtg tcccaaaggt tgagaacaat cgccaaagcc agataaatca ggttaacggt
     9841 ccacatccac agcgtcgggt ccagcttacc tttaccgccg ttcgatttca gggaggtctg
     9901 gatcaggaag aaaagtagat acagcagcat ggctggcagc atcgacagta cgcgtccctg
     9961 acgcgggtta accacgctca acggtacaac cataagtgcc atcataaaca cggtgaatac
    10021 caacgtgata cgccagttca gttctgcgcg ggcacgatcg gtgtcagtgt tccacaatgt
    10081 gcgcatgtcc atctggtcgg tatcgttcgg gtcgagcacc accgcctggt gaccaatgat
    10141 cgcctgataa tcctgaaagt ccgtaatgcg gaaatcgcgt aacagtgcag tgccttcgaa
    10201 gcgcgttccc tggttgagag tgacgacctg ggagccgtcg cgcagctggg ttaaatgtcc
    10261 ggaatcggcc accaccacag aaggacgtgc attacctttt ggtcgaattt gtgcgaggaa
    10321 cacatctttg aaatcgctgc cgtcaacgct ttcgatgaac agcaccgagc tgccattagt
    10381 cgcttgctgg aattgccctt gcgccagcgc cgccatgcca gggttcgctt tcgcttctgc
    10441 taacacttca tcctgatgac gcgatgacca tggtcccgcc cacatcacgt taaccgccgc
    10501 gacgattgcc gtgaacaccg caaggatcat tgccgctttc accagcaccg ctttgctcag
    10561 accgcaggca tgcattaccg taatttcact ttcggtatac agtttgccca gcgtcatcag
    10621 cagcccgagg aacaggctta atggcaggat aagctgcgcc atttccggca cgcccaaccc
    10681 gagaagggag agcaccagat tcgccggaat atcgccgtca accgctgcgc cgaggatcct
    10741 cactaacttt tgacagaaga agatcaaaag caagatgaag agtatcgcca gctggctttt
    10801 gagcgtctcc cgcaccagat atcttatgat tatcacttta aatacgcccg taaaaactcg
    10861 tcttttgcag gattttagct tgtttcatgg cttaaacgtc atttattctc ttgagtcgtc
    10921 gaaatcgtcg ctaagataat tatactcaac ggattcacct ctcagatttt gttctgacgt
    10981 gccaatgccg taataacgtt aagattaaca cgaagtcatc gcaacagcgg acatgagtta
    11041 cgaaagcttg caattctatc tgtagccacc gccgttgtct ttaagattca ggagcgtagt
    11101 gcatggagtt tagtgtaaaa agcggtagcc cggagaaaca gcggagtgcc tgcatcgtcg
    11161 tgggcgtctt cgaaccacgt cgcctttctc cgattgcaga acagctcgat aaaatcagcg
    11221 atgggtacat cagcgccctg ctacgtcggg gcgaactgga aggaaaaccg gggcagacac
    11281 tgttgctgca ccatgttccg aatgtacttt ccgagcgaat tctccttatt ggttgcggca
    11341 aagaacgtga gctggatgaa cgtcagtaca agcaggttat tcagaaaacc attaatacgc
    11401 tgaatgatac tggctcaatg gaagcggtct gctttctgac tgaactgcac gttaaaggcc
    11461 gtaacaacta ctggaaagtg cgtcaggctg tcgagactgc aaaagagacg ctctacagtt
    11521 tcgatcagct gaaaacgaac aagagcgaac cgcgtcgtcc gctgcgtaaa atggtgttca
    11581 acgtgccgac ccgccgtgaa ctgaccagcg gtgagcgcgc gatccagcac ggtctggcga
    11641 ttgccgccgg gattaaagca gcaaaagatc tcggcaatat gccgccgaat atctgtaacg
    11701 ccgcttacct cgcttcacaa gcgcgccagc tggctgacag ctacagcaag aatgttatca
    11761 cccgcgttat cggcgaacag cagatgaaag agctggggat gcactcttat ctggcggtcg
    11821 gtcagggttc gcaaaacgaa tcgctgatgt cggtgattga gtacaaaggc aacgcgtcgg
    11881 aagatgcacg cccaatcgtg ctggtgggta aaggtttaac cttcgactcc ggcggtatct
    11941 ccatcaagcc ttcagaaggc atggatgaga tgaagtacga tatgtgcggt gcggcagcgg
    12001 tttacggcgt gatgcggatg gtcgcagagc tacaactgcc tattaacgtt attggcgtgc
    12061 tggcaggctg tgaaaacatg cctggcgggc gtgcctatcg tccgggcgat gtgttaacca
    12121 ccatgtccgg tcaaaccgtt gaagtgctga acaccgatgc cgaaggccgc ctggtactgt
    12181 gcgacgtgtt aacttacgtt gaacgttttg agccggaagc ggtgattgat gtggcgacgc
    12241 tgaccggtgc ctgcgtgatc gcgctgggtc atcatattac tggtctgatg gcgaaccata
    12301 atccgctggc ccatgaactg attgccgcgt ctgaacaatc cggtgaccgc gcatggcgct
    12361 taccgctggg tgacgagtat caggaacaac tggagtccaa ttttgccgat atggcgaaca
    12421 ttggcggtcg tcctggtggg gcgattaccg caggttgctt cctgtcacgc tttacccgta
    12481 agtacaactg ggcgcacctg gatattgcag gaaccgcctg gcgttctggt aaagcaaaag
    12541 gcgcaaccgg tcgtccggta gcgttgctgg cacagttcct gttaaaccgc gctgggttta
    12601 acggcgaaga gtaagtttgc gttttaccct caccctaacc ctctccctaa gggagaggga
    12661 accgatcgag cgcaatagaa attcattgct catctttcag ggtgaggaat gcagcaatat
    12721 ttaaatccac cacaagaagc cccatatatg aaaaacgcaa cgttctacct tctggacaat
    12781 gacaccaccg tcgatggctt aagcgccgtt gagcaactgg tgtgtgaaat tgccgcagaa
    12841 cgttggcgca gcggtaagcg cgtgctcatc gcctgtgaag atgaaaagca ggcttaccgg
    12901 ctggatgaag ccctgtgggc gcgtccggca gaaagctttg tcccgcataa tttagcggga
    12961 gaaggaccgc gcggcggtgc gccggtggag atcgcctggc cgcaaaaacg cagcagcagc
    13021 ccgcgcgata tattgattag tctgcgaaca agctttgcag attttgccac tgctttcaca
    13081 gaagtggtag acttcgttcc ttatgaagat tctctgaaac aactggcgcg cgaacgctat
    13141 aaagcctacc gcgtggctgg tttcaacctg aatacggcaa cctggaaata atggaaaaga
    13201 catataaccc acaagatatc gaacagccgc tttacgagca ctgggaaaag cagggctact
    13261 ttaagcctaa tggcgatgaa agccaggaaa gtttctgcat catgatcccg ccgccgaacg
    13321 tcaccggcag tttgcatatg ggtcacgcct tccagcaaac catcatggat accatgatcc
    13381 gctatcagcg catgcagggc aaaaacaccc tatggcaggt cggtactgac cacgccggga
    13441 tcgctaccca gatggtcgtt gagcgcaaga ttgccgcaga agaaggtaaa acccgtcacg
    13501 actacggccg cgaagctttc atcgacaaaa tctgggaatg gaaagcggag tccggcggca
    13561 ccattacccg tcagatgcgc cgtctcggca actctgtcga ctgggagcgt gaacgcttca
    13621 cgatggacga aggcctgtcc aatgcggtga aagaagtttt cgttcgtctg tataaagaag
    13681 acctgattta ccgtggcaaa cgcctggtaa actgggatcc gaaactgcgc accgctatct
    13741 ctgacctgga agtggaaaac cgcgaatcga aaggttcgat gtggcacatc cgctatccgc
    13801 tggctgacgg cgcgaaaacc gcagacggta aagattatct ggtggttgcg actacccgtc
    13861 cggaaaccct gttgggcgat accggcgtgg ccgttaaccc ggaagatccg cgttacaaag
    13921 acctgattgg caaatatgtc attctaccgc tggttaaccg tcgtattccg atcgttggcg
    13981 acgaacacgc cgacatggaa aaaggcaccg gctgcgtgaa aatcactccg gcgcacgact
    14041 ttaacgacta tgaagtgggt aaacgtcacg cgctgccgat gatcaacatc ctgacctttg
    14101 acggcgatat ccgtgaaagc gcccaggtgt tcgataccaa aggtaacgaa tctgacgttt
    14161 attccagcga aatcccggca gagttccaga aactcgagcg ttttgctgca cgtaaagcag
    14221 tcgttgctgc ggttgacgca ctcggcctgc tggaagaaat taaaccgcac gacctgaccg
    14281 ttccttacgg cgaccgtggc ggcgtagtta tcgaaccaat gctgaccgac cagtggtacg
    14341 tgcgtgccga tgtactggcg aaaccggcgg ttgaagcggt tgagaacggc gacattcagt
    14401 tcgtaccgaa gcagtacgaa aacatgtact tctcctggat gcgcgatatt caggactggt
    14461 gtatctctcg tcagctgtgg tggggtcacc gtatccctgc atggtacgac gaagcaggta
    14521 acgtttatgt tggccgcaac gaagaagaag tgcgcaagga aaataacctc ggtgccgacg
    14581 ttgccctgcg tcaggacgaa gacgttctcg acacctggtt ctcttctgcg ctgtggacct
    14641 tctctaccct aggctggccg gaaaacactg acgcactgcg ccagttccac ccaaccagcg
    14701 tgatggtatc cggcttcgac atcattttct tctggattgc ccgcatgatc atgatgacca
    14761 tgcacttcat caaagatgaa aatggcaaac cgcaggtgcc gttccacacc gtttacatga
    14821 ccggcctgat tcgtgacgac gaaggccaga agatgtccaa atccaagggt aacgttattg
    14881 acccgctgga tatggttgac ggtatttcgc tgccggaact gctggagaaa cgtaccggca
    14941 atatgatgca gccgcagctg gcggacaaaa tccgtaagcg caccgagaag cagttcccga
    15001 acggtattga gccgcacggc accgatgccc tgcgcttcac cctggcggcg ctggcgtcta
    15061 ccggtcgtga catcaactgg gatatgaagc gcctggaagg ttaccgtaac ttctgtaaca
    15121 agctgtggaa cgccagccgc tttgtgctga tgaacacgga aggtcaggat tgcggcttca
    15181 acggtggtga aatgacgctg tcgctggcgg accgctggat tctggcagag ttcaaccaga
    15241 ccatcaaagc gtaccgcgaa gcgctggaca gcttccgctt cgatatcgct gcaggcattc
    15301 tgtatgagtt cacctggaac cagttctgtg actggtatct ggagctgact aagccggtaa
    15361 tgaacggtgg caccgaagcc gaactgcgcg gtactcgcca tacgctggtg accgtactgg
    15421 aaggtctgct gcgcctcgcg catccgatca ttccgttcat caccgaaacc atctggcagc
    15481 gtgtgaaagt actttgcggt atcactgccg acaccatcat gctgcagccg ttcccgcagt
    15541 acgatgcatc tcaggttgat gaagccgcac tggccgacac cgaatggctg aaacaggcga
    15601 tcgttgcggt acgtaacatc cgtgcagaaa tgaatatcgc gccgggcaaa ccgctggagc
    15661 tgctgctgcg tggttgcagc gcggatgcag aacgtcgcgt aaatgaaaac cgtggcttcc
    15721 tgcaaaccct ggcgcgtctg gaaagcatca ccgtgctgcc tgccgatgac aaaggtccgg
    15781 tttccgttac gaagatcatc gacggcgcag agctgctgat cccgatggct ggcctcatca
    15841 acaaagaaga tgagctggcg cgtctggcga aagaagtggc gaagatcgaa ggcgaaatca
    15901 gccgtatcga gaacaagctg gcgaatgaag gctttgtcgc ccgcgcaccg gaagcggtca
    15961 tcgcgaaaga gcgtgagaag ctggaaggct atgcggaagc gaaagcgaaa ctgattgaac
    16021 agcaggctgt tatcgccgcg ctgtaatcaa aacacagtga tgaaaacgaa ggccggagca
    16081 tgctccggcc ttttttatct cttacagaaa tggtaaagaa ggcataattc cccgtgaaat
    16141 actggctaag aatcctttat ctggttgttc ttctttattg attaaaggaa ggtcatccaa
    16201 atcgcccagc aaatacgtat tttttgcctg ccagtcaatc atccatattt tcagcagtgg
    16261 ataagccaga ccgcccgtaa tcccggatat caccaccaac gcacacatgc gataaagcat
    16321 accgtggtat gttaaagttg agcgaaaacg aatacgccca tcattcagtg acaggttgct
    16381 cataaaatga tttcgcaacg acaccgttaa ataactggtg ctaacagcaa tcccaaaata
    16441 atagattaac tgcgcgatta tcattttacg ttgcatttcc ataaattgct gtaaattctc
    16501 aatatcatca tttgcatacc cagaactatc atactcattt aatatttgat cgaagataat
    16561 ataaccagca acagcgagaa aaggtaataa agccaaaaat gcgagaatgg catattttat
    16621 acaatatgta gcatctaatt tcaccttgaa acgatgtata ccgaaactgg tattactcca
    16681 gagaaaactc attaccagac tatataaagt gccattaaaa ataccaatga aaacaataca
    16741 aactattgtc atcagaacca cagatataat aacgctactt gaactattgg catgtagcat
    16801 ctttgtcgag ataaagaaaa cagtccccat cccaagggcc attaaaatcg gcaagaaaaa
    16861 ggtcacccac cagaaccctt tcatagagca attaaaacta aatcttacgc cgttgagact
    16921 ggtcatcaag gcctgataac gcagtccttt tgctgccata aatataagca aaaccaacag
    16981 tgacaaagtc aaaacacagc caataagcgg catatctgct gacactgtca ttaagattgc
    17041 gaaatagaaa aaaacaaaaa caagacaact aacaaaaaca ttcccaccgg taatcccata
    17101 agaaaatcgt tgtccgttaa cttccatatt agcatagaga taacgcttac atttcattaa
    17161 tgcccatggt aaatagatac ctagcgttat aatcgttaac aacacattca ccacacaaat
    17221 aagaaaatat ctctcacctg taccatgaaa aacaaacgaa tgggacggaa catccatatc
    17281 attaataact tgagccataa aagttccctg aattaaaaaa tccattgata taatttatat
    17341 atacagacag taaaatgttc tgcc
//
LOCUS       DAJVRF010000083        17002 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJVRF010000083 DAJVRF010000000
VERSION     DAJVRF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17002
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.083"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="OQN13_002535"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="OQN13_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1615864.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="OQN13_002536"
     CDS             2219..2767
                     /locus_tag="OQN13_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCS1615865.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="OQN13_002537"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="OQN13_002537"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCS1615866.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="OQN13_002538"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="OQN13_002538"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCS1615867.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSSLINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="OQN13_002539"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="OQN13_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCS1615868.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OQN13_002540"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OQN13_002540"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCS1615869.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8301..9503)
                     /gene="pncB"
                     /locus_tag="OQN13_002541"
     CDS             complement(8301..9503)
                     /gene="pncB"
                     /locus_tag="OQN13_002541"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCS1615870.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9769..12381
                     /gene="pepN"
                     /locus_tag="OQN13_002542"
     CDS             9769..12381
                     /gene="pepN"
                     /locus_tag="OQN13_002542"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCS1615871.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLEEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12588..13355)
                     /gene="ssuB"
                     /locus_tag="OQN13_002543"
     CDS             complement(12588..13355)
                     /gene="ssuB"
                     /locus_tag="OQN13_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1615872.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13352..14143)
                     /gene="ssuC"
                     /locus_tag="OQN13_002544"
     CDS             complement(13352..14143)
                     /gene="ssuC"
                     /locus_tag="OQN13_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCS1615873.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(14155..15302)
                     /gene="ssuD"
                     /locus_tag="OQN13_002545"
                     /pseudo
     CDS             complement(14155..15302)
                     /gene="ssuD"
                     /locus_tag="OQN13_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15299..16258)
                     /gene="ssuA"
                     /locus_tag="OQN13_002546"
     CDS             complement(15299..16258)
                     /gene="ssuA"
                     /locus_tag="OQN13_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCS1615874.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            complement(16251..16826)
                     /gene="ssuE"
                     /locus_tag="OQN13_002547"
     CDS             complement(16251..16826)
                     /gene="ssuE"
                     /locus_tag="OQN13_002547"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCS1615875.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
BASE COUNT         4250 a         4408 c         4330 g         4014 t
ORIGIN      
        1 ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgt tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttctatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattatccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gagctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcagtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag ctcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggaacttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaatg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcggggttg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcctcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tggggggggg gatacctatg ttacctggca tctgcaatca
     8221 gacaagcaga attcgcaaat gcagcgaaag atttcggaat taaaggtaaa aaaagggagc
     8281 cgattagctc cctgatgata ttaactggct tttttaatat gcggaaggtc gaacgctttg
     8341 cgcagcgccc gaacaaacgc tttatcatgg cagatagttt tgccagggct gtcagaaagt
     8401 ttcgccaccg gtttaccgtt acactctacc aacttaatca caatattcag gggttttacc
     8461 tgggggatat cgcaggtcag gcgagtccca ataccaaaac ttaattgcac gcgggaagag
     8521 aagtggcggt atagctcaac cgctttgcgt aaatccagat tgtcagagaa aaccagcgtt
     8581 ttactctgtg gatcaattcc cagcttttca taatgtgcaa tggctttttc accccattca
     8641 accgggtcgc cagagtcatg acgcaggccc tgataccgac tagcgaactc gacaccgaaa
     8701 tcacgcagga aagcatccat agtgatgcag tcggttaatg caatgccaag ttggtcggga
     8761 tactcttcca gccaggcagc aagtgcagct cgctggctgt tggctagatc cgggctgatt
     8821 tgctgatgtg cctggaacca ttcgtgtgcc tgtgttccca tcggcgtgag ggaaagccga
     8881 cgcgccagat cgtagttgct ggtgcccacg aaccaggatt cctgttgcag acgcttaacg
     8941 atggtttctt gtacttcgcg agaaaaacgg cgacgggtgc caaaatccat cagatggaag
     9001 cgcgacatat caagaccggc ggttaacgcc gagaagtcga ctaatttgct ttccagcgtg
     9061 tcgagggctt gcgcaacgtc ggcctgcggt gagcgatagc gatgtaccat ttcactgata
     9121 accgccagca aaggaacttc ccagaggatg acttcacgcc acggaccgct taagcgaata
     9181 tccagcttgc cattatcgtt ggacacggtg acttgttccg ggttaaagcg gaactcgcgt
     9241 aaccagttaa gatagtcggc cttaaagaaa ggcagggcag agagccactg atattcatca
     9301 tcctgcaggc gcaggtgctg catcgcctga atctgttcac gaatagcatc ggcataaata
     9361 cccagcagat cgtcacctcg gcaacgaaac tccgccgcga catgcacatc gtaatagtga
     9421 tgaaacacgg cttgctgcat atgcaactta taagcatctg tatccagcaa cgagtgcaga
     9481 acaggagaag cgaattgtgt cataggtgcg cagtagcgtc ctcgtacagg agcgtttagt
     9541 acaataaaca tcttcaggaa ctgctggagt ataccttgtt tcgcaattta ttgaaccccg
     9601 atcacaccat atgccacctt tctggtcgat ggcattacgc ggtgtatgtt ataaaaatgt
     9661 agcaataaag gcgtttgtac ctgaaaagat gaagattctg catagcgcga tttacgcaac
     9721 aggaatagac tgaacaccag actctataaa agatgctaaa ggttatttat gactcaacag
     9781 ccacaagcca aataccgtca cgattatcgt gcgccagatt accagattac tgatattgac
     9841 ttgacctttg acctcgacgc gcaaaagacg gtcgttaccg cggtcagcca ggctgtccgt
     9901 catggtgcat cagatgctcc acttcgtctc aacggcgaag acctcaaact ggtttctgtt
     9961 catattaatg atgagccgtg gaccgcctgg aaagaagaag agggcgcact ggtcatcagt
    10021 aatttgccgg agcattttac gcttaagatc gttaatgaaa taagcccggc ggcgaatacc
    10081 gcgctggaag ggctttatca gtcaggcgat gcgctttgca cccagtgtga agccgaaggt
    10141 ttccgccata ttacgtatta tctcgaccgc ccggacgtgc tggcgcgttt taccaccaaa
    10201 attattgccg ataaaaccaa atatcccttc ctgctttcca acggtaaccg cgttgcgcaa
    10261 ggtgaactgg aaaacggacg ccattgggta cagtggcagg acccgttccc gaaaccgtgc
    10321 tacctgtttg cgctggtggc aggcgacttt gatgtactgc gcgacacctt taccacgcgt
    10381 tctggtcgcg aagtggcgct ggagctgtac gtcgatcgcg gcaaccttga tcgcgcgccg
    10441 tgggcgatga cctcgctgaa aaactcaatg aaatgggatg aagaacgctt cggcctggag
    10501 tatgacctcg acatctatat gatcgtcgcg gtggatttct tcaatatggg cgcaatggag
    10561 aataagggtc tgaatatctt taactccaaa tatgtgctgg cccgcaccga caccgccacc
    10621 gacaaagatt acctcgatat tgaacgcgtt atcggccatg aatatttcca taactggacc
    10681 ggtaaccgag tgacctgccg cgactggttc cagctcagcc tgaaagaagg tttaaccgtc
    10741 ttccgcgatc aggaattcag ctctgacctt ggttcccgcg cagttaaccg catcaataat
    10801 gtgcgcacca tgcgcggatt gcagtttgcg gaagacgcca gcccgatggc gcacccaatc
    10861 cgcccggaca tggtcattga gatgaacaac ttctacaccc tgaccgttta cgagaagggc
    10921 gcggaagtga tccgtatgat ccacaccctg ctgggcgaag aaaacttcca gaaagggatg
    10981 caactctatt tcgagcgtca tgatggtagc gcagcgacct gtgacgactt tgtgcaggcg
    11041 atggaagatg cgtcgaatgt tgatctctcc catttccgcc gttggtacag ccagtccggt
    11101 acgccgattg tgaccgtcaa agacgactac aatccggaaa ctgagcagta caccctgacc
    11161 atcagccagc gcacgccagc cacgccggat caggcagaaa aacagccgct gcatattccg
    11221 tttgccatcg aattgtatga caacgaaggc aaagtgatcc cgttgcagaa aggcggtcat
    11281 ccggtgaatt ccgtgctgaa cgtcacccag gcggaacaga cctttgtttt tgataatgtc
    11341 tacttccagc cggtgcctgc gctgctgtgc gaattctctg cgccagtgaa actggaatat
    11401 aagtggagtg atcagcaact gaccttcctg atgcgtcatg cgcgtaatga tttctcccgt
    11461 tgggatgcgg cgcaaagttt gctggcaacc tacatcaagc tgaacgtcgc ccgtcatcag
    11521 caagggcagc cgctgtctct gccggtacat gtggctgacg ctttccgcgc ggtgctgctc
    11581 gatgagaaga ttgatccggc gctggcggca gaaatcctga cgctgccttc tgtcaatgaa
    11641 atggctgaac tgttcgatat catcgacccg attgctattg ccgaagtacg cgaagcactc
    11701 actcgtactc tggcgactga actggcggat gagctgctgg ctatttacaa cgcgaattac
    11761 cagagcgagt accgtgttga gcatgaagat attgcaaaac gcactctgcg aaatgcctgc
    11821 ctgcgcttcc ttgcttttgg tgaaacgcat ctggctgacg tgctggtgag caagcaatac
    11881 catgaagcaa acaatatgac cgatgcgctg gcagcgcttt ctgcggcggt tgctgcacag
    11941 ctgccttgcc gtgacgcgct gatgcaggag tacgacgata agtggcatca ggacggtctg
    12001 gtgatggata aatggtttat cctgcaagcc accagcccgg cggcgaatgt gctggagacg
    12061 gtgcgtggtc tgttgcagca tcgctcattt accatgagca acccgaaccg tattcgttcg
    12121 ttgattggtg cgtttgcggg cagcaacccg gcagcgttcc atgccgaaga tggcagcggt
    12181 taccagttcc tggaggaaat gcttaccgac ctcaacagcc gtaacccgca ggtagcttca
    12241 cgtctgattg aaccgctgat tcgcctgaaa cgttatgatg ccaaacgtca ggagaaaatg
    12301 cgcgcggcgc tggaacagtt gaaagggctg gaaaatctct ctggcgatct gtacgagaag
    12361 atcaccaaag cactggcttg atacttttga gccaaaatgc ctgatgcgac gcttgacgcg
    12421 tcttatcagg cctacaactc cgttgaaatt gctggttttg taggctggat aaggcgttca
    12481 cgccgcatcc ggcagccgtg cccaactgcc tgatgcgatg cttgacgtgt cttatcaggc
    12541 ctacaagtcc gttgaaaatg ctggttttgt gcgccacatc cggcatatta accatgacgc
    12601 cgaatcagtt gttcagaatg cccacgccgc attacccgtt gcaatacttc cgcttccagc
    12661 tccgctaatc gcaccgaccc taatcgacgc ggacggggga tatccactgt caaatccaga
    12721 ccaatttttc cctcttcaat taacaacacc cggtcagcca ttgctaccgc ttcgctcaca
    12781 tcatgcgtca ccaacagtac ggtaaagccg tgctgctgcc aaagtgacac aatcaaatcc
    12841 tgcatctcga gtcgcgttaa ggcgtccagt gccccgagcg gttcatcaag caacaataaa
    12901 cccggtcgat gaatcaacgc tcttgccagc gccacacgct gtttctgccc gccagaaagt
    12961 gcggcaggcc attccccggc gcgattctcc agtcctactg cagccaacgc ccgacgcgcg
    13021 gcatcgcgcc actggccttt aaggcctaac ccaacgttat caatcaccga tttccacggc
    13081 agcagacgcg catcctgaaa catcattcgc gtgtcttcct gaatctcagc cagcggtgtg
    13141 gtgcccgcta acacatcgcc tgcggttggc gtttccagac ctgccagcag gcgcagcagg
    13201 gtacttttgc caccaccgct gcggcccacc accgccacaa actgacctgc cggaatatgt
    13261 aaatccagtt ggttcaggac gatattttcc gcgtaatgtt tgcttactgc attgagcaac
    13321 aatggtgtgc cctggttcag acgagcagta ttcataccgt ggcctccttc aaatgataag
    13381 ccgggttcca gcgcaaccag aggcgctcta acagctgcgc gctgacgtca gcaagtttgc
    13441 cgagcagggc gtaaagaata atagcgacta ccaccacgtc cgtttgcaaa aactcccgcg
    13501 cattcatcgc cagataacca atgcctgaat tggcagaaat ggtttcggca acaatcagcg
    13561 tcagccacat caggcccaac gcaaaacgca cgccgaccat aattgagggc agggcaccag
    13621 gcaggatcac atggataaac agcggtatgc cggataatcc atagctacgc gccatttcca
    13681 ccagaccgcg atcgatatta cggatgccat gccaggtgtt gatataaatg gggaatagcg
    13741 tgccgagcgc caccagaaag atttttgcgg attcatcaat gccaaaccac aaaatcacca
    13801 gcgggatcag cgccagatgc ggtacgttgc gcaacatctg aattgaggta tccagcagcc
    13861 gttctcccca gcgtgacagc ccgctaatca gtcccagaat caagcccagc gatccgccaa
    13921 ttgaaaagcc aatcagcgcc cgccaggaac tgatcgccag atgctgccac aattcgccgc
    13981 tggcggagag cgtccagaac gccgtcacca ctccttccgg tgaaggcaaa atacgcgtcg
    14041 acagccagcc aaccgaggag gccagttgcc acaccgccac gatgcccacc ggtaaaaacc
    14101 agggggcaac gcgcaataac cacttcttca ctggcgttgc catcatcgtg ttccttagct
    14161 ttgcgcgact ttacggggga taaaatcatt cgccaccgct tcgccttgcg gattcagcgg
    14221 ctgcggctgg ggaatttccg gaatggcgac atccagatgt gggaacagca actcgccaac
    14281 ccgatacgct tcttccagat gcgggtagcc cgaaagcaca aagctgtcga tgccaagcgc
    14341 ggcgtattcg ttgattcgcg cggcgaccgt aggaccatcg cccaccagcg ccgttccggc
    14401 accgccgcgc actaagccaa cgcccgccca taaattgggg ctgatctcca gattgtcgcg
    14461 cttgccgtta tgtagcgccg ccattcgctg ttgtcctacg gaatccgtcc gggcgaatgc
    14521 ggcctgtgct ttggcgatag tttcatcatc aagatgcgag attaaccgct cggcggcctg
    14581 ccacgcttcg tcgttagttt cacgaacaat cacatgcaga cgaataccga aacgaatttt
    14641 gcgtccatgc gcggcagctt tcgcccgcac ttgttcgatc ttctctttta ccagctctgg
    14701 cggttcgccc caggtgaggt agagatcaac ttgttctgcc gccagctcct gggcgacatc
    14761 tgacgatccg ccaaagtaaa gcggaggata cggctgttga atcggcggga agagcagttt
    14821 tgctccccgc acatgaatat gtttaccgtt gaaatcgacg ctttccccca gcaataagcg
    14881 ccccgccaga cctgggtaaa ttccgccgag gcttcgtagc gctcgctatg atcaaggaac
    14941 acgccgtcgc ctgccaactc ttgtggatcg ctgcctgtga ccagattaaa caacgcacga
    15001 ccatttgaga gacggtcaag cgtggcggcc tggcgagcgg caacggtagg tgaggttacg
    15061 ctgggacgca gggcgacaag aaacttcagc cgctgcgtca ccgggatcat cgacgccgcc
    15121 accagccacg catcttcaca cgatcgcccc gttgggatca gcacaccggt atagcccaga
    15181 cgatccgccg cttgcgcaat ttgttgcaga tagccgtgat caaccgggcg tgaaccttct
    15241 tccgttccaa gataatgccc gtcaccatgg gtcggtaaaa accagaacat attcagactc
    15301 ataattgttt tccttccagg tgagtgggct gccagatgcg ctggcgaata tcaacttttt
    15361 tcgacaccag acggttttca taaaacagat ctgccgtttg ctgctgtaag gcggcaactt
    15421 cagcgctcac cggtttgatg gtggtgggag gacgatgatc cagatacgag gcaatcaccg
    15481 gtgccggtaa gcccatcgtt tttgccagta aagcgatgct ttgctcgcgc tggctgcggg
    15541 ttaacgcatc ggcctcacta aaggttgcca gtacgccctg aataaaagcg ccgttttttt
    15601 ctgcataggg gcgagctgcc agataaaacg atccagtttg attgagatcg gtgccgtctt
    15661 tcagcacccg cacgccgccc tgtaataacg cagcggagta gtagggatcc cagatagccc
    15721 aggcgtcaac gttaccttgc tggaacgcgg cgcgggcatc agctggcgtc agataagtgg
    15781 gctggatgtc agtaaactta agtccggcct gacgcagtgc acgcagtaaa aggttgtgtg
    15841 aactggaacc tttctgaaag gcaactttgt gacctttaag atcggctacg gttttgatcg
    15901 ggctgttttc tgccaccaga atcacttcgg ctttgggctt cggtggctcg acgcccacat
    15961 atatcaaatc agccccggca gcctgggcaa agattggcgg aatatccccg gtactgccga
    16021 gatcaatact gccaacgttt aacgcttcca acatttgcgg acccgcgggg aactccaccc
    16081 aggagatttt tgattgcgga tagcgttttt ccagtaactg gtggcttttt gccaatacca
    16141 taccaatact gcctttctga tagcctatac gtaacgcttc aggcgaggat tctgcagcaa
    16201 ccgcaaaagt agagacgcta agcaatcccg ccagcgccag tttaatgatg ttacgcatgg
    16261 acattacctc gcagagacag aaggtcagga acctgaacat cgcggcggtg caatgcctgc
    16321 cagaaagttt ctagcgcggt atcaagacgg gtttgcagat ttggcgtgaa ctggggtttg
    16381 tgatggtaat caataacttg tgagtcatcg gcaaacacgc cgtgcaggag ctcctgggct
    16441 ttcatcgcac ttaaaactgg cttaagggca taatcgaccg ccagcagatg agccaccgta
    16501 ccgcccgtcg ccagcggtag caccactttg ccttgcaaag cgcgctctgg cagcaggtcg
    16561 agcagggttt tcagcgcgcc ggaataggcg gctttataca caggtgtagc gactatcagt
    16621 ccatcagcct gttgcagctg ttcggtgaag gtcttgagtg ccggactatc gaaacgagcg
    16681 taaagcagat cttccggggc gaagttttgc agattccagt gataaacctc tacatccagg
    16741 ccatttagtt tttcccgcgc atattccagc aaggagctgg agcgagaagg aaagcgagga
    16801 ctacccgcca gggtgatgac acgcatactc tctccttata accaattgtt ctttttttgt
    16861 taacattgat aacaattcgg tcagtctgtc ggagagacaa gaaaatgcca aatataaatt
    16921 ttgtgtatct ttttctgaaa ataaactaaa gaaagggctt tatatatcac gcatatttat
    16981 ttgttggata gtcattagat gt
//
LOCUS       DAJVRF010000084        16791 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJVRF010000084 DAJVRF010000000
VERSION     DAJVRF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16791
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.084"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(143..1306)
                     /gene="emrK"
                     /locus_tag="OQN13_002548"
     CDS             complement(143..1306)
                     /gene="emrK"
                     /locus_tag="OQN13_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCS1615876.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            1722..2336
                     /gene="evgA"
                     /locus_tag="OQN13_002549"
     CDS             1722..2336
                     /gene="evgA"
                     /locus_tag="OQN13_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCS1615877.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            2341..5934
                     /gene="evgS"
                     /locus_tag="OQN13_002550"
     CDS             2341..5934
                     /gene="evgS"
                     /locus_tag="OQN13_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCS1615878.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(5990..7135)
                     /gene="yfdE"
                     /locus_tag="OQN13_002551"
     CDS             complement(5990..7135)
                     /gene="yfdE"
                     /locus_tag="OQN13_002551"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCS1615879.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(7209..8153)
                     /gene="yfdV"
                     /locus_tag="OQN13_002552"
     CDS             complement(7209..8153)
                     /gene="yfdV"
                     /locus_tag="OQN13_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCS1615880.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(8223..9917)
                     /gene="oxc"
                     /locus_tag="OQN13_002553"
     CDS             complement(8223..9917)
                     /gene="oxc"
                     /locus_tag="OQN13_002553"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCS1615881.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(9971..11221)
                     /gene="frc"
                     /locus_tag="OQN13_002554"
     CDS             complement(9971..11221)
                     /gene="frc"
                     /locus_tag="OQN13_002554"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCS1615882.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(11734..12357)
                     /locus_tag="OQN13_002555"
     CDS             complement(11734..12357)
                     /locus_tag="OQN13_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCS1615883.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            12654..12929
                     /gene="ypdI"
                     /locus_tag="OQN13_002556"
     CDS             12654..12929
                     /gene="ypdI"
                     /locus_tag="OQN13_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HCS1615884.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(13006..13248)
                     /locus_tag="OQN13_002557"
     CDS             complement(13006..13248)
                     /locus_tag="OQN13_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCS1615885.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            13601..14521
                     /gene="lpxP"
                     /locus_tag="OQN13_002558"
     CDS             13601..14521
                     /gene="lpxP"
                     /locus_tag="OQN13_002558"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCS1615886.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            14877..14948
                     /gene="ypdK"
                     /locus_tag="OQN13_002559"
     CDS             14877..14948
                     /gene="ypdK"
                     /locus_tag="OQN13_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCS1615887.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(15013..16251)
                     /gene="alaC"
                     /locus_tag="OQN13_002560"
     CDS             complement(15013..16251)
                     /gene="alaC"
                     /locus_tag="OQN13_002560"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCS1615888.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            16628..>16791
                     /locus_tag="OQN13_002561"
     CDS             16628..>16791
                     /locus_tag="OQN13_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCS1615889.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSL"
BASE COUNT         4756 a         3877 c         3519 g         4639 t
ORIGIN      
        1 agaaagccag atattgtcgg tattgcgacg ttagaaatag tggaatccaa catttgcata
       61 aatgtcgcta atgacaatgc aatagtgacg caccataacg tcccaccggt taatggtgcc
      121 ggagttgatt tagtgattgc cattaaagtt gtccattatg cgaaataatg ttgctaattt
      181 ctttttctat cggactggta tcgataacta aagccttact ggtataagcc ggcatggagg
      241 tcacggttga agccagctca ggcatctcag caatgtcttc gtccttcgta tcaatagttg
      301 cagtcatcga taaaccaatg cgcaaggggt gttccatgag ttcttttgga tcaagagaaa
      361 cttcaaccgg tacacgctga acgattttga tccagttccc tgtcgcattt tgtgcaggta
      421 ataaggagaa cgcattgccg gttcccatat tgatccctgt cacccgacca tgaaacacaa
      481 cattttcacc ataaagatcg ctgataatat tgactgattg accaatccgt acatccgtga
      541 gttgtgtttc tttaaagttg gcattaaccc acatttgacg tgccggtacg acagccatta
      601 acgattgtcc gggactcact gtttcgccga cctgaacact tcgctgggca atatagccag
      661 taaccggact ccttatatcc gtacgtttaa gcgccaacca ggcttctttg gttgcatccg
      721 ccgcttcaac tacttgtggc tgacggttta atggtgtgtt cattactaaa gctttattcg
      781 ctttataagc ctggatagcg gcattcagtg ccgctttgct acttattaac gtatctttgg
      841 tatgctccag cgtttctttt gaaataaccc cctgcttcgc taaaggcaca cgacggttat
      901 aatcttctaa ggattgttga tactgaatac gtgctgaagc gacttcggca ctgtattgtt
      961 tatcctgtaa gtacagttta ttcgtttgcc gaacaatatt tgccagatta tttttagctt
     1021 tattgagtgc gatagtggca tcagttttat ccagtgaaac taaaatgtca ccctgtcgaa
     1081 cgtagttcgt atctttatga ttaactacag tgacactacc tgagacttgt gcagaaattg
     1141 gatctgcatt ccccgtgaca taggcgtcat ctgtactaat catgtcttct aattccattg
     1201 accaataggc ataggcacct gaaaacgcaa taaataaaac tatcaccaat aaagaaaagt
     1261 atttttttct gttagactgt tttttatttg aattaatctg ttccactatt atctctcatt
     1321 tcgcatagat gaaatttatg aattgaatga aaagagtatt cattcttcat atcaaacaca
     1381 gtgcatgtgt tgcacattat cccaggaacg ttggaggcct aatttaaatg aggtgcaaaa
     1441 aaaacaacag aggactaaac cgtggctttt gcaatacaat tcttacgcct gtaggattag
     1501 tgagaagact tatagtgcta acttgaaact ataaatcatc ggtataatcc ctgattttat
     1561 tgttgacatt tcatttatgc cgactattta tatggtatac ttgtcgaatt atcttaaagg
     1621 aagctcagat tttcttattt ttattgagaa aatgagatga cgccttatgt ctgtattact
     1681 acagggagaa gggagatgct tcattgcaaa gggaataatc tatgaacgca ataattattg
     1741 atgaccatcc tcttgctatc gcagcaattc gtaatttatt gatcaaaaac gatattgaaa
     1801 tcttagcgga gttaactgaa ggcggaagcg ccgttcagcg ggtggaaaca cttaagcctg
     1861 atatcgtcat cattgatgtc gatatccccg gagttaacgg tatcctggtg ttagaaacgc
     1921 tgaggaagcg ccaatatagc ggaattatta ttatcgtctc cgctaaaaat gaccattttt
     1981 acgggaaaca ttgtgctgat gctggcgcta atggtttcgt gagtaaaaaa gaaggcatga
     2041 acaatatcat tgcggctatt gaagctgcaa aaaatggcta ctgctatttc cccttctctc
     2101 tcaaccggtt tgttggaagt ttaacgtccg accagcaaaa actcgactcc ttatcgaaac
     2161 aagaaattag tgtcatgcgg tatattcttg atggcaagga taataatgac attgcggaaa
     2221 aaatgttcat cagcaacaaa actgtcagca cttataaaag tcgcctgatg gaaaaattag
     2281 aatgtaaatc actgatggat ctttacacat tcgcacaacg taacaaaatc ggctaaccac
     2341 atgaagtttt taccctatat ttttcttctc tgttgtggtc tttggtcgac cataagtttc
     2401 gcagacgaag attacatcga atatcgtggc atcagtagta acaaccgtgt catacttgat
     2461 ccagtacgtc tgagcaacaa ggaattacgt tggttagcga gcaaaaaaaa tcttgtgatt
     2521 gcagtacata agtcccaaac ggctacgttg ttgcataccg attcgcagca acgagttcgt
     2581 ggtattaatg ctgattattt aaatctttta aaaagaacgt taaatatcaa attaacactc
     2641 cgggaatacg ccgatcatca aaaagcaatg gacgcgctgg aagaaggtga agtcgatata
     2701 gtgttatcac atttagtagc ttcgccgcct cttaatgatg acattgctgc aaccaaacca
     2761 ctgataatta cctttccggc gctggtaacc acccttcacg attcaatgcg accgcttacc
     2821 tcatcaaaac cagtaaatat tgctcgagta gcaaattatc cccctgacga ggtaattcat
     2881 caatcatttc caaaagcaac aattatctct tttacaaatt tatatcaggc attagcatcc
     2941 gtctcagccg gacagaatga ttactttatt ggtagtaaca tcattaccag cagtatgatt
     3001 tcccgctatt tcactcactc cttaaatgta gtgaaatatt ataactcacc gcgtcaatat
     3061 aattttttct tgaccagaaa agaatctgtc gttcttaatg aagtactcaa tcgatttgtt
     3121 gatgctttga caaatgaagt tcgctatgaa gtatcacaaa attggcttga tacaggaaac
     3181 ctggcctttc tgaacaaacc attagaactc actgaacatg aaaaacaatg gattaagcag
     3241 catcccgatt taaaggtgct ggaaaatcct tactcccctc cctattctat gacggatgaa
     3301 aatggctcgg ttcggggcgt tatgggggat attcttaata ttattacttt gcaaacaggt
     3361 ttaaattttt ctccgatcac cgtttcacac aatatccatg ctggaacaca gctgaatccc
     3421 ggcggatggg atatattacc cggtgctatt tatagtgaag atagagaaaa taatgtttta
     3481 tttgctgaag ccttcataac aacgccttac gtttttgtca tgcaaaaagc gcctgacagt
     3541 gaacaaacat taaaaaaagg aatgaaagtt gccattccat attattatga gcttcattcg
     3601 caattaaaag agatgtatcc ggaggtagag tggataaaag tcgataacgc cagcgctgca
     3661 tttcacaagg tcaaggaagg cgaacttgat gctctggtcg cgacacagtt aaattcacgt
     3721 tacatgatcg accattacta tcctaatgaa ctttatcatt ttcttattcc cggcgttccg
     3781 aatgcatcgc tttcgttcgc ttttcctcgc ggagaaccgg aacttaagga tattattaat
     3841 aaagcactga atgcaatccc cccaagcgaa gttctgcgcc tgaccgaaaa atggattaaa
     3901 atgcccaatg tgaccattga cacatgggac ctctatagcg agcaatttta tattgttacg
     3961 acattatccg ttttattagt tggcagtagc cttttatggg gattttacct gttacgctca
     4021 gttcgtcgtc gtaaagtcat tcagggtgat ttagaaaacc aaatatcatt ccgaaaagca
     4081 ctctcggatt ccttaccgaa tccaacttat gttgtaaact ggcaaggtaa tgtcattagt
     4141 cataatagtg cttttgaaca ttatttcact gcggattact acaaaaatgc aatgttacca
     4201 ttagaaaaca gtgactcccc ctttaaagat gttttttcta atgcgcatga agtcacagca
     4261 gaaacgaaag aaaatcgaac aatatacaca caggtttttg aaattgataa tggcatcgag
     4321 aaaagatgca ttaatcactg gcatacatta tgcaatctgc cagccagcga tcatgctgtt
     4381 tatatttgtg gttggcaaga tattacagag acgcgtgatt taattcatgc actcgaagta
     4441 gagagaaata aagcgatcaa tgcaactgtc gcaaaaagtc agtttctggc aacaatgagt
     4501 cacgaaataa gaacaccaat aagctccatt atgggcttcc tggaacttct gtcgggttct
     4561 ggtcttagca aggagcaacg ggtggaggcg atttcacttg cctactccac cggacaatca
     4621 ctcctcggct taattggtga aatccttgat gtcgacaaaa ttgaatcggg taactatcaa
     4681 cttcaaccac aatgggtcga tatccctact ttagtccaga acacttgtca ctctttcggt
     4741 gcgattgctg caagcaaatc gatcgcatta atttgcagca gtacatttcc tgatcattac
     4801 ctggttaaga tcgaccctca ggcgtttaag caggtcttat caaatttact gagcaatgct
     4861 ctcaaattta ccaccgaggg ggcagtaaaa attacgacct ccctggttca cattgatgac
     4921 aaccacgctg ttatcaaaat gacgattatg gattctggaa gtggattatc gcaggaagaa
     4981 caacaacaac tctttaaacg ctacagccaa acaagtgcag gtcgccagca aacaggatct
     5041 ggcttaggct taatgatctg caaagaatta attaaaaata tgcagggcga tttgtcatta
     5101 gaaagtcatc caggcatagg aacaacattt acgatcacaa tcccggtaga aattagccag
     5161 caagtggcga ctgtcgaggc aaaagcagaa caccccatca cactacctga aaagttgagc
     5221 atattaatcg cggatgatca tccgaccaac aggctattac tcaaacgcca gctaaatcta
     5281 ttaggatatg atgttgatga agccactgat ggtgtgcaag cgctacacaa agtcagtatg
     5341 caacattatg atctgcttat tactgacgtt aatatgccga atatggatgg ttttgagttg
     5401 actcgcaaac tccgtgagca aaattcttcc ttacccatct gggggcttac agccaacgca
     5461 caggctaacg aacgtgaaaa agggttaagt tgcggcatga acttatgttt gttcaaaccg
     5521 ttgaccctgg atgtactgaa aacacattta agtcagttac accaggttgc gcatattgca
     5581 cctcagtatc gccaccttga tatcgaagcc ctgaaaaata atacggcgaa cgatctacaa
     5641 ctgatgcagg agattctcat gactttccag catgaaacgc ataaagatct acccgctgcg
     5701 tttcaagcac tagaagctgg cgataacaga actttccatc agtgtattca tcgcatccac
     5761 ggtgcggcta acatcctgaa tttgcaaaag ttgattaata ttagccatca gttagaaata
     5821 acacctgttt cagatgacag taagcctgaa attcttcagt tgctgaactc tgtaaaagaa
     5881 cacattgcag agctggacca ggagattgct gttttctgtc agaaaaatga ctaaatagcg
     5941 gctcccacaa tgttctaatg tgggagctat ttaccagcac atcttacatt tatgatgaga
     6001 actcctggcg aatttgttcc ccatgctggt cgagcgttgc cgctcccggc ataacatgcg
     6061 ggtccgcgca gccgctgatt tttatcggat tacccggcat cattattcct ccggcttcaa
     6121 tcaacatatt tctcgcctga gtttgtggca aattaatggc ctcagccaca cttaataacg
     6181 gcgcgacggg cacaccaact tcatgtattt tggctaacca aacttcagct gcctgcgttt
     6241 ttaacgttcg ctcaatatat tgtttaagaa tagcctggtt ttgtacgcgt aaaatattgc
     6301 tgctaaatcg gggatcatta accagttccg taagctccag tgcctggcat aacgcagaaa
     6361 aaagcttgtc attaccacaa caaatcgtaa tcggcttatc ctgagtatcg aaaacatcaa
     6421 aaggtgccat gtagggatgg cgatttccca ggcgttgtgg tgacttccct gtcgcaatat
     6481 atgccatcag accatgctcc agaaaactca gcgtggcatc aaacatcgct atatcgacat
     6541 gcgcccctct ctggctcttt tcgcggccat aaagtgcact cactattccg ctgaataaat
     6601 agacaccgcc gcatagatcc gcaagagagg taccaacacg cactggcgga gcatcagggt
     6661 accctgtttc catcattatc ccactcattg cctgaatgat ggtatcgtag gcaggagcat
     6721 cttttagcgg accggtatgt ccgaaacctg acgatgaagc atatatgagg cgcgggttga
     6781 tttcttgtaa cctttcccat gaaaacccca gtttttccat tgtacctggg cgaaaattct
     6841 cagctaatac atcagcttgt ttgagcatat ttataaatat acttttatcg tgatcattct
     6901 ttaaatcaag aaccacactc tctttgccat gattaataaa actgtaatag agtgactgtc
     6961 catccacata gggaccaaat gtgcgggtat catcaccatg acctggtggc tcaactttaa
     7021 ttacccttgc gcccatatta caaagaagtt gagttccgaa aggtccatta aggacatgtg
     7081 tcatatcgat aactaataag ccttcaaacg gccctttgct ttcattattt gtcatttgct
     7141 tatgccttga aaaaagccga aggtgaaaag acacttcggc atttatgtaa aatcaataac
     7201 atgattaatt atgaaaccag tcgactgaca taaatccaca agggagccgt gacgacaaag
     7261 cccagtacgc tcacagccaa tgacgctgta ccagtacggg tgtagacatt aaaccggctg
     7321 gcaataatga ttccggagaa tgccggcggt aatgcgcctg ccagtaccat catctgcaaa
     7381 tgttcgctgt tcaaatgaca tgccatacca acgagaagca gcgccagtgg catcagaatc
     7441 aacttcagga aggtgttata agcaatttcg gcactgaact cgaatttatg tgcagccaga
     7501 gtcaaccccg cagcgaatac cgctacccct gagttagcct tcgcaatcag attaaaggtc
     7561 gggtcccatg ctgccggaat ttttacccca accagcacca ggatcgttgc cagaacaggt
     7621 gcccatacta ctggctcctt tgccgcagaa attaatgcgc tcagattact attcttctta
     7681 ccatccgctc ctgaggaagg attcagcaaa tacagaccaa taggaatagt aattgcgtta
     7741 acaataatag aaataattgc taccactaaa cctgttgata cggaatcacc ataaatagga
     7801 tcgagaactg caaaccccag gaatccaatg gtaggtgaac ccgcaattaa tgcacataca
     7861 gcggcttctg catgggtacg tttaaaaaat ttgtagcaac caaaccaaga gaaaaagaaa
     7921 catccgacaa taaccacaag tgatacaagg gtcagacgag tgtccgcaaa aatcatttcc
     7981 ctgtttgccc gggtaataga aacaaataga gccgcaggaa gagcgtagtt taataccagt
     8041 ttattaaatg cgcgggcttg atcttctgaa aaagtttctc gtctgccgct aaaataaccc
     8101 aacagcatga ttacgataat aggcaataaa tcgccaataa aaaatgttag cataatagta
     8161 tttccgataa gggtcccctc accatcgcat aatgagttgg caagaggaga cgatgtcagt
     8221 atttaattac cagcgacttg ttttgggtta agtttggtaa tatggccact ttcagttcct
     8281 gcagcagggt cgatgaccac attaataatg gtcggtttgc gcgactgaat accggtggtt
     8341 aaagcatgac gaagttcatc tgtcgtggtg acgttatagc caacgccacg aaacgcatcc
     8401 attaatttgt cataccttgc atggtgcaac agatccgttg gtgatggtgc accagcgcca
     8461 ctgagatcaa caccgtctcc tctgtagatg ccgccattat taaaaataac gatcgtcacc
     8521 ggcaggttat atcgacaaat cgtttcaatt tccatcccac tgaaaccaaa agcactatca
     8581 ccttcaatgg cgacaaccgg agaaccagaa gtcacgctag caccgatggc atagcccata
     8641 ccgatgccca tgacacccca ggtaccacaa tccagacgac gacgtggttt atacatatca
     8701 ataatatttc gtgcattatc cagggtattt gcaccttcat taactaaata aatatcctgg
     8761 ttctcgcgca atacgtcgcg cacagcactt aatgcattaa agtaatttaa tggttgagta
     8821 tctgtactta atttttcatg cattttttgt gcattttgct gcttgtggat atttaaaata
     8881 tcgcgccata ccagtggagt cgtaaatgtg ttttgtttca gttctgccag cataccttgc
     8941 atactggatg caatatcacc aacgactggc acagcaatgg ggcggttgct gtcaatttcc
     9001 tgcggttcaa tatccagttg aataaactgt gtatctgccg cccatccttt tttaccgtgt
     9061 gccaataacc aattcagtcg tgcaccaaca agcatgacaa cgtcagcatt tgccagggca
     9121 aacgaacgcg cagctgccgc agaaagtgga tgcgtatctt caaggatccc tttcgccata
     9181 gacattggca ggaatggaat ctgagcactt tcaataaatt cacgaagctg ttcatcagct
     9241 tgtgaatacg ccgcgccttt gccaaggata attaatggcc gttcagcttt tgctaaaagc
     9301 gaaattgcgc tagtgactga cttcgggcat ggcaataatg ctggcgacgg attttcaact
     9361 ttaacaatcg tggttaacgc ttcgtctttt tccatcgtcg cggccaggac atttgctggc
     9421 aaatcaagat aaactccgcc agggcgaccc gatacagaga cccggatagc gcgtgccaat
     9481 gcaatgccaa gatcctgcgg ctgattaacg cgaaatgctg ctttggcata cggttttgcc
     9541 gcattcattt ggtccagctc ttcataatca ccttgctgta ggtcgacgat cgcgcggtcg
     9601 ctggagccgc taatcatgat catcggaaaa ccatttaccg ttgcgttggc caatgcggtc
     9661 aaaccattga ggaatcctgg cgcagaaact gtcaggcaga tccccggttt ttgggtaaga
     9721 aaaccgcttg ccgcagcggc atagcctgcc gactgctcat gacgaaaacc aatataacga
     9781 atgccttccg cctgggcatg gcgtgccata tccgtcacag gaatacctac aacaccataa
     9841 atagtgtcaa tattattctg ttttaatgct tcaacgatga tatgcatacc atctgtcatt
     9901 tgaagttgat ctgacatgat taataacctc gaaattttta taaattggga gccagaagcc
     9961 cccgttaata tcagatggcg tggttttgct tcattgcagc aatttcatcg tcgctataac
    10021 ccagctcctg caatacagca gcggtatgtt cacctaatag cggcgcagct ttaatatccg
    10081 gcgtaaaggc agagaatttc attggacaac caacggtcag atattttcca cgcaacggtt
    10141 gttccacttc aacaacactg ccactttggc gcaaagaggg atcaagtgaa atttctttca
    10201 tacttaaaac cggtgcacaa ggaatatcaa actgagtcaa ataggccacc gcttcatgtt
    10261 tatcaatagt gacagtgtat ttttcgattt cagcaaaaat atcgaaaata tgtggctgtc
    10321 gggcatgggc tgtactgtat gccgaatcgg taatccattc tggtttgccg atggctttac
    10381 aggtgttttc ccagttttgc tcctgaatag tgaaataaat ataggcgtta ggatcggttt
    10441 cccagccttt acatttcagg atccagccag gctgaccgcc accacctgca ttaccaccgc
    10501 ggggaactgc atcaccaaat gtaccattcg gatactgcgg gtattcttcc agataaccca
    10561 atttatcgag acgctgctgg tcacgtaatt tcacgcggca aaggttcaat acggcatcct
    10621 gcattgacat ggtgactcgt tgcccacgcc ccgttttttc gcgatgcagc aaagcagcaa
    10681 gtaaaccgat cagcaaatgc attccggtgt tgctgtcacc caacgctgca gcgcttacca
    10741 gcggcggacc atcccaaaaa cccgtagtgg atgccgcgcc acccgctgcc tgagcaacgt
    10801 tttcataggc ttttacattc acataaggcg aacactcatc aaaccctttg atcgaaccaa
    10861 aaatcagacg tggattgatt tcttgaatat gctcccaggt gaagcccatg tgatcaatgg
    10921 cccctggatg aaagttctcg actaagatat cagcttcgcg gatcagcttt tccattacct
    10981 ctttgccttc cgctgttttg gtatttaact caatagaacg tttgttactg ttaagcatgg
    11041 tgaagtaaag cgcatcgata tcaggaatat ctcgcagttg gtgacgcgtt acgtcaccaa
    11101 cgccgggacg ttcaatttta ataacgtcag cgccaaacca ggccagcatt tgagtacaag
    11161 atgggccaga ttgcacaccg gtgaaatcga gaactttaat tccttgaagt ggagttgaca
    11221 tagcaatacc tattagttaa ttaaatatgt gttaatttag cgtttattta aggcataacg
    11281 ccctctataa ataatctgca aaatatcttc gtagcggcac tcgatatttt taaattcctg
    11341 tatcaagtac tgaaaattat ttcatcgcca cttccaattc ctttctgcat ggtaagtagt
    11401 gaggacgaaa agaagaatat gataaatatt tattcgtggt aatcagagag agtcgcgaca
    11461 tttgtgatgt tattcttaca actgtaagaa gcgccggtga tttacaagta agagtatgat
    11521 tacgcttttt aaagcgtacc ataaagcata acataataaa tgatttgtga tgacactgga
    11581 ttattgcggt ttactttgag gaaaataaaa tgcgtgttta tagaaccgcg agcgccaaat
    11641 aattttatga ccacaacctg tcgtattgtg gtcataaaaa attgtcggct ggagggagtt
    11701 aaatccttta cctcaatata ctgcctgtca gcattagttc acaaacagtg cttcgctatc
    11761 gacgatgatt ccatcttccg cacctttaag cgccaggttt gcctcgtagt attgcttttt
    11821 atcaagcagt ttctgcgcat cagcaagtgc attacgagtc tgtttaagcg gcatcagata
    11881 ttggttttcc attacgccaa caccggccag gcgaagctct tccattgcgc cctttttatc
    11941 ccctttcgcc attttttcat tggcaatttt tatcgcggct tctttttcgg gtgttgctac
    12001 ataactctca gaaattccga cagatgcatt aatgacaata tattggtcat cattgagatt
    12061 ggtcttttta cctggcttag cgaactttgc ccattcggta ctgtcatctg acagcaaagc
    12121 ggaagcttca ttggtcagtt cttttgcctt ttccggatcg ccatgaaaca gagccaggcg
    12181 cgctacctgc acgtcacgca tcgcatataa accttgttca gagatacgct ctgctgctgc
    12241 cgttttctgg gtttgctgag tttgctgttg tgccgctacc ggtgcattat cagcagccca
    12301 tacagttgaa gatgccagaa ttgctgtgac catcgtagcc ataattaaac gtttcatact
    12361 tacctcattc tttaacagtt ttagaaaacg ccgccatcat attgggtgag catgaaatga
    12421 gaaatcagag agcaacggtc attttgaaag ggttatctta caattgtagt tattatcgca
    12481 aagcggcatt tagtcttaca gttgtaggaa tatgcttcct gtcgcagaga actattctta
    12541 tataccacaa agagatgtca tttattatat tgatgtaaat attgattaat tatatgtaaa
    12601 tacagccata acatcgtatg gttctcagtg aaccattttc aggataactt tctatgaaag
    12661 taaacttaat acttttcagc ttatttttat tggtctctat tatggcatgc aatgtttttg
    12721 cattttccat ttctggtggt gtaagtgaga ggagctataa agagactgaa aaaacatcag
    12781 cgatgacaac cacacactct acaaaacttc agccatcaca ggcgattttg tttaagatga
    12841 gagaagatgc gccaccatta aacctcacag aagaaatcac gcctacttat ccgacaaagg
    12901 cgaattatct tattcatcct gtgcgatagc gtctgtcaca tggcaggtga acaatcatgt
    12961 ctgatgctga tgtgaacacc tgcccaaatg agccatcctg acccactatc gcctgccata
    13021 ccccaccata aagcgaatca tcaagccaca agccggggca agaaacataa atatccccgt
    13081 caccagttca ttaccgccag ttaaccacat ggtgaagtaa taaatgagtg cagcgaggat
    13141 caccatgccg aagaaagaag agaccgccga taccacgttc agtacctgac cgatgtagcc
    13201 gcttacgcag acaatacaca gggcgagaaa catccataaa taaatcatag ctcaacctcc
    13261 tgccgcgcta aaaaacctgt aacttcagtc actttaatag taagacaatc cttaacctct
    13321 ggcaattttt gctaagcatc aatgaaaaca gcacgttaaa tgcaacgcta ttgcgtgcgg
    13381 aaaatttttt actttgcacg attaataatc agtagctgaa agcagtcagc cagtgataaa
    13441 gtagagccac cgcattcatg ctcctcgtgg ttatgtcctg actagtcttt acactcttta
    13501 caggaaccat tgtcgtacat gatggcccaa ccaattgagt gtaccgctgt cgacatggaa
    13561 tcttctgcga tacaacaatt cgtatctaca gaaggtaact atgtttccac aatgcaaatt
    13621 ttcccgcgag tttctacatc ctcgctactg gctcacatgg tttgggcttg gtgtactctg
    13681 gctttgggta cagcttcctt atcctgttct ctgctttctc ggcacgcgta ttggcgcaat
    13741 ggcgcgacca ttcctgaaac gtcgtgaatc tatcgcccgt aaaaacctgg aactttgttt
    13801 cccgcagcat tctgcggaag aacgcgagaa gatgattgcc gaaaactttc gttcactcgg
    13861 catggcgctg gtagaaaccg gcatggcatg gttctggccc gacagtcgcg tacgtaaatg
    13921 gtttgatgtt gaagggttgg ataaccctaa acgcgcacaa atgcaaaatc gcggcgtaat
    13981 ggttgtcggc gtccatttta tgtcgctgga actgggcggc cgcgtgatgg gactgtgcca
    14041 accaatgatg gctacctatc gtccacataa taatcagctg atggaatggg tgcagacccg
    14101 tgggcgcatg cgctctaaca aagcgatgat cggcagaaat aatctgcgcg gcattgtcgg
    14161 tgcactgaag aaaggtgaag cggtatggtt tgctcccgat caggattatg gtcgtaaagg
    14221 cagctccttc gcgccgttct ttgcggtgga aaatgtcgcc acaaccaatg gcacctatgt
    14281 tctctcccgt ctctctggcg cagccatgtt gaccgtaacg atggtaagaa aagcggatta
    14341 cagcggatat cgtttgttta tcaccccaga gatggaaggc tacccgacag atgaaaatca
    14401 agccgctgcc tatatgaaca agattatcga gaaagagatc atgcgcgcac cggagcagta
    14461 cctctggatc caccgtcgct ttaaaacgcg cccggtggga gaatcgtcgt tgtacattta
    14521 acaaactgct cttactcttt ccctcctcaa atcggccata ataacctcat ggtttttatg
    14581 gcttttttct taaggttttt tccttcgctg aatatctaat tattaccgtc agaaattaaa
    14641 ctgtcgccga tatgagacac ttgtaagtta ctaaaagtaa ataagaaaat gcctcttgtc
    14701 tcgtttcagt tttaggcgta cattaacgcc gtcttgagca gtttagctca gaattctggg
    14761 gtgatgccca cggtaaaata cagggctttt aaggtcgctt tgcccggtgt caactcactg
    14821 tatcaggtgt aatgaagtca ttcaggcgta acagtaatta cgcggagaga tgtaaagtga
    14881 aatatttctt tatgggcatt tcttttatgg tcatcgtttg ggccggtact tttgccctga
    14941 tgatctaaaa gcaaactgtg caatgaaaaa caggagccat caggctcctg tttgcatttc
    15001 tgacgacggt gtttattccg cgttttcgtg aatatgtttg ctgctggcgg gtagtaaacc
    15061 gtcggcacgg aacatcgctt taatccctct gatggcctga cgaatacggt cgcggttttc
    15121 aatcagtgca aagcgaacat gcgtatcacc atagtcgcca aagccaatcc ccggcgagac
    15181 acaaaccttc gcttcgttaa ggagcttctt ggcaaattcc agcgatccca tggccgcata
    15241 tggttccggg attttcgccc agacatacat cgaagccttc ggcatttcga ccatccagcc
    15301 cgcttcatgc agccctttaa ccaatacatc acggcggcgt ttgtactgtt cggtaatgtc
    15361 gcgcacgcac tgttgatcgc cctcgagtgc cgcaatcgcc gccacctgca acggcgtaaa
    15421 agtgccgtaa tcgtgatagc ttttgattcg cgccagggca ctgaccagcg ttttgttgcc
    15481 gaccataaag ccgatacgcc agcccgccat gttgtagctt ttcgacagcg taaagaactc
    15541 gaccgccaca tcgcgtgcac ccggcacctg cataattgac ggcgctttcc agccatcgta
    15601 gacgatatcg gcataggcca ggtcatggac caccagcaca tcgtagcgtt tcgccagcgc
    15661 cacaaccttt tcgaagaact ccagttccac gcattgcgcg gttgggttcg acgggaagcc
    15721 gaggatcatc atcttcggtt tcggataact ttcacgaatg gcgcgctcca gttcgttgaa
    15781 gaaatcgaca ccttccacca gcggcactga gcgtacctgc gccccggcaa tcaccgcgcc
    15841 ataaatatgg atcgggtaac ttggattcgg caccagcacc gtgtcaccat gatccagcgt
    15901 cgccagcatc agatgcgcca ggccctcttt cgaaccaata gtgacgatgg cttctgattc
    15961 cgggtcgatt tcaacgtcgt agcgatcctg ataccagcgg gaaatggcgc gacgtaaccg
    16021 cggaatgccg cgtgaagtgg agtaaccatg cgtgtccggg cgctgggcca cagtacataa
    16081 tttttcgacg atatgcggcg gagtcgcacc gtccgggtta cccatgctga aatcgataat
    16141 atcttcgccg cgccgacgcg cagccatttt cagttcagcg gtgatgttaa aaacgtaggg
    16201 cgggagacga tcaatgcgcg taaagcgacg ttcagggcga gtgtcagcca taatttcctc
    16261 agattaacgt tagcgcccgg accgtccgag cgacgctgcc acaatggtgg ctgttttgaa
    16321 aatagcctga ttaatttctg tctgtccagc gttttagaac atttttatcc atggctttaa
    16381 atgaggaact ccacttcaaa aacaggaaat ttttctacgc taacaagaaa attaccacca
    16441 attttattaa cattataatt tcaataaatt taccagccag aagtgcatta tcaagagata
    16501 cattgctaat acaccattta tctttcaatc cccttttaaa tttatggttt ttcctcaaaa
    16561 acggcctgct ggtcatcagc cttcaggtta cctatcatag aggtttaatc cttattcaga
    16621 gtcacccgtg cacgaaatat tcaacatgct gctggcggtc ttcgatcggg cagcgttaat
    16681 gcttatctgc ctgttctttc tcatccgtat ccgcctgttt cgcgaactgt tgcacaagtc
    16741 ggcgcactcc ccaaaagaat tgctcgccgt taccgccatt ttctcgctgt t
//
LOCUS       DAJVRF010000085        16583 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJVRF010000085 DAJVRF010000000
VERSION     DAJVRF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16583
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.085"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..576)
                     /gene="araH"
                     /locus_tag="OQN13_002562"
     CDS             complement(<1..576)
                     /gene="araH"
                     /locus_tag="OQN13_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCS1615890.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLN"
     gene            complement(591..2105)
                     /gene="araG"
                     /locus_tag="OQN13_002563"
     CDS             complement(591..2105)
                     /gene="araG"
                     /locus_tag="OQN13_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCS1615891.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(2175..3164)
                     /gene="araF"
                     /locus_tag="OQN13_002564"
     CDS             complement(2175..3164)
                     /gene="araF"
                     /locus_tag="OQN13_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCS1615892.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            3961..4464
                     /locus_tag="OQN13_002565"
     CDS             3961..4464
                     /locus_tag="OQN13_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCS1615893.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(4543..4794)
                     /locus_tag="OQN13_002566"
     CDS             complement(4543..4794)
                     /locus_tag="OQN13_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCS1615894.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(4909..4995)
                     /gene="azuC"
                     /locus_tag="OQN13_002567"
     CDS             complement(4909..4995)
                     /gene="azuC"
                     /locus_tag="OQN13_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCS1615895.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            5258..5581
                     /locus_tag="OQN13_002568"
     CDS             5258..5581
                     /locus_tag="OQN13_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCS1615896.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            5752..6249
                     /gene="ftnA"
                     /locus_tag="OQN13_002569"
     CDS             5752..6249
                     /gene="ftnA"
                     /locus_tag="OQN13_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCS1615897.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(6287..6526)
                     /locus_tag="OQN13_002570"
     CDS             complement(6287..6526)
                     /locus_tag="OQN13_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCS1615898.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            6717..7928
                     /gene="tyrP"
                     /locus_tag="OQN13_002571"
     CDS             6717..7928
                     /gene="tyrP"
                     /locus_tag="OQN13_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCS1615899.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(7990..8655)
                     /gene="yecA"
                     /locus_tag="OQN13_002572"
     CDS             complement(7990..8655)
                     /gene="yecA"
                     /locus_tag="OQN13_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCS1615900.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(8851..8937)
                     /locus_tag="OQN13_002573"
     tRNA            complement(8851..8937)
                     /locus_tag="OQN13_002573"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8901..8903),aa:Leu,seq:taa)
     gene            complement(8950..9023)
                     /locus_tag="OQN13_002574"
     tRNA            complement(8950..9023)
                     /locus_tag="OQN13_002574"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8989..8991),aa:Cys,seq:gca)
     gene            complement(9078..9153)
                     /locus_tag="OQN13_002575"
     tRNA            complement(9078..9153)
                     /locus_tag="OQN13_002575"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9118..9120),aa:Gly,seq:gcc)
     gene            complement(9305..9853)
                     /gene="pgsA"
                     /locus_tag="OQN13_002576"
     CDS             complement(9305..9853)
                     /gene="pgsA"
                     /locus_tag="OQN13_002576"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCS1615901.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(9910..11742)
                     /gene="uvrC"
                     /locus_tag="OQN13_002577"
     CDS             complement(9910..11742)
                     /gene="uvrC"
                     /locus_tag="OQN13_002577"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCS1615902.1"
                     /translation="MSDQFDAKAFLKTVTCQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(11739..12395)
                     /gene="uvrY"
                     /locus_tag="OQN13_002578"
     CDS             complement(11739..12395)
                     /gene="uvrY"
                     /locus_tag="OQN13_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCS1615903.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            12691..12867
                     /locus_tag="OQN13_002579"
     CDS             12691..12867
                     /locus_tag="OQN13_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1615904.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            12854..13078
                     /locus_tag="OQN13_002580"
     CDS             12854..13078
                     /locus_tag="OQN13_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCS1615905.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(13145..13867)
                     /gene="sdiA"
                     /locus_tag="OQN13_002581"
     CDS             complement(13145..13867)
                     /gene="sdiA"
                     /locus_tag="OQN13_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCS1615906.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(14097..14849)
                     /gene="yecC"
                     /locus_tag="OQN13_002582"
     CDS             complement(14097..14849)
                     /gene="yecC"
                     /locus_tag="OQN13_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCS1615907.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(14846..15514)
                     /gene="tcyL"
                     /locus_tag="OQN13_002583"
     CDS             complement(14846..15514)
                     /gene="tcyL"
                     /locus_tag="OQN13_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCS1615908.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(15529..16515)
                     /gene="dcyD"
                     /locus_tag="OQN13_002584"
     CDS             complement(15529..16515)
                     /gene="dcyD"
                     /locus_tag="OQN13_002584"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCS1615909.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT         4188 a         4277 c         3839 g         4279 t
ORIGIN      
        1 attcagcaac aaaccaaaga taatcagaca cgcgacggtg agccagattg gcgcaggcag
       61 accgaaccag ttagcgtaac caagggcaaa gaagctttca tcttcgatac cgaccgcttt
      121 accgtctgaa atgatgtacg ccagacctcg aacaatctgc atcgttgcca gtgttgtgat
      181 cagagcattt attttcagtt tggcgataac aaagccattg accaggccac agagaatgcc
      241 aagcagcaac cccgctgcca cgccaatcca caggctttca gtcaggttga taaccaccgc
      301 cgtggtgaca cccgcacagg caattacgga ggcgacagaa aggtcaaagt caccggaagc
      361 aaggcagaac aacatgccac acgccaccat ccccgacatg gaaattgcca ggcccaaccc
      421 tttcatatta atgaaggtgg caaaatttgg gacaaaaatg gcacaggcga taaagagcac
      481 cgcaaacacc accagcatgc cgtactgatc ccagatacgc ccgaagctga atgacgactt
      541 aggtgcgcca gaacccgatg tagaaacaga agacatcata ctctccttac tcaggcaact
      601 gcctggctga ctttaggcat cgcaaggctc agtgcctgac gctcatctgc ctgctcgtgt
      661 aacaattcac cggcgatttc accttcccgc atcaccacaa tccggtcggc aacgccgagg
      721 acttcaggta agtcgctgga ggcaaacagc accgccacac cctgcgccgc cagcgcataa
      781 attacgttat atatttcgtg cttagcacca acatcaatgc tgcgcgtagg ttcatccagc
      841 aaaatgacct tcatctcttc cgataaccag cggcccagaa tggctttttg ctgatttccg
      901 cctgagagat tcatgatcag ttgctcagcg cccggcgttt tgatgttgag cgaacgaatg
      961 tgctgatcgg cattgttttc ttcccaaccg ttgttgatta cacaaccgcc gagcacatgt
     1021 ttgcgtctgg cactgatgtt gatattgtcg cgaacggagt gcacgggaat aatgccttcc
     1081 gctttgcgat cttccgggca gagcatcatg cctgcggcaa tggcgtggct cggtttacga
     1141 atatcgatcg gctgttggtc gatataaacc tgaccggcgg tgatttgcgt cccgccaaac
     1201 aagcctttca ttaattcgct acgccccgcc cctaccagac caaacagccc aacaatttca
     1261 ccactgcgaa ccgccagact tattggcgta cgcacgcctg gtgctttcac agcatcaaga
     1321 cgcaggcgct cctcgccata actacgaggt tgccagccgt agatatcgcc aatgtcgcgc
     1381 ccgaccatcg cctgcaccag cgcgtcgtgg tcaacctgct gcatatcggt aaaggttttg
     1441 acataacgtc catctttaaa gacagtgatg gcatcgctga gggcaaatat ttcttccata
     1501 cggtgagaaa cgtataagat cacccgcccc tcttttcgca gttcacgaat aacgcggaaa
     1561 agattgtcga tttcgcgggc agaaagggag ctggttggct catcaaaggc gataatttta
     1621 gcgttacgcg ccagcgcctt ggcgatttca accatctgcc actgaccaat ggagagatat
     1681 ttcagcggcg tgtccgggtc aatatccata ccaagatgtt taagttgtaa acccgcctca
     1741 taattcagca atgagcgatt cacaatgccg cctttatgcg gcagctggcc gagatagatg
     1801 ttttccgcga cggtcatttc cggcacgaga tgcagttcct ggtaaataat cgccacaccc
     1861 gcgttaagtg ctgcggtcgt gtcggaaaag gacatttcct gcccattaat cactacagaa
     1921 cccgtggttg gcgcatagtt gccgctgagg atttttaaga gagttgattt tcctgcgcca
     1981 ttttcaccca tcaacgcatg aacctgaccg gcatagcagt caaaactaat atccgtcagc
     2041 gccttaacgc cgggaaacgt tttaccgatg ccgcgaaatg agagatacgg ggtagactgt
     2101 tgcataacgt ctccgtgaat cactggtcgt tcacaccccc tctgcatcat gcagagggga
     2161 atttttccgg caaattactt accgcctaaa ccttttttct ccagttcttc tttaaagttg
     2221 tcacgcgtga tcagcaccac gtcggtaact tcggtaaatt ttggcggttc aacgtctttt
     2281 gctacccagt tgtaaagcat ttcgctggat ttatagccat gtacgtccgg gcttggcagc
     2341 agggaaccgt agaagccggt tgcctgtgct ttagacagtt cgctcaacgc atccacaccg
     2401 ttaatgccaa tgccgatgat atcggcagct ttaaagccct gaccttccgt cgcgcgtacg
     2461 ccgcccagca cggtgctgtc gttcataccg acgatcagcc aatgtttaac ttccggatgt
     2521 tgaaccagca ttgagttggc agcgtcaaat gcccccggga tgtcgttaga tttggtaggt
     2581 acctgataaa tttgtttttc cgggaatccg gccgctttca gcgcatccat agaacccgtt
     2641 gtacggcgac gtgccgtatc cagttcgttg gcggtaatcg ccatcaccgc gctttctttg
     2701 acatcccagc cacgtttctg catctcttta tacagttcct gaccctgacg ttcgccaatt
     2761 ttagtcgccg ccatcatcac cagcggaacg gtatccattg gcttaccttt ggcgttaaca
     2821 aactggtcat ccacggcaat gactttcata tcgtagccac gcgctttcgc gacgatggca
     2881 gagccgagtt tggggtccgg agtacaaata acgaaacctt ttgcgccact ggcagccagg
     2941 ctgtcgatcg cgttcaatgt tttttcgcca tccggcacgg caatcttaat aacctcaaac
     3001 cctaaatcct tcccggcttt atcggcaaac ttccattcgg tctggaacca cggctcttcc
     3061 ggttgcttca ccagaaaacc gagcttcagg ttctccgcca tagcggattg tgacataacg
     3121 gctgccagac caatggctgc cagggcttta gtaaatttgt gcatggttct ctccagcttt
     3181 agtgtcgttt tgtgtagggc aaaaacgaat gactttcgtt aaattaatcg gaaaacaaag
     3241 cattaccttt tatctaaaag ataagtgacc gtgttgacat agttttagcg agaaattaat
     3301 tctccatagg agagcaatat cacatcgcag aattacagtg agaacgtgca taaatttagc
     3361 gggaaaagac ataagggaaa gccaatttgt cagacaaatt gtcgaatgca cagcagatta
     3421 atccataaga ttagcctgga aatccttgtt gtctttggta cccatgcggg atgtcttctt
     3481 tttaaccagt caataggccg cattacctgg cgttgagttt ttgaaatggt gtaataaccg
     3541 caactcaaag atgtggaaaa tgcacgtcat tcatttcgcc attaattatc actgtgctca
     3601 ttaattaaca gaacacgtat aatgagagcc atctcgcaaa aatgaaaaaa cgttttataa
     3661 aatcatcact tcatcatgaa ttcaaattca ttgattaata tcaacaagat acaaaaagca
     3721 ctatcattaa aattcattgc agttacattg atttcatcaa tgaaatgtaa aaatatataa
     3781 acttgatgat ttaagcattt tcttataccc gttcagacgt tattcttatt tcagatcatc
     3841 gtcagaattg actccacgat cacatttcgg accggcagaa aggaattatt ctgcaaacag
     3901 taattatggt gttttgattt atcttgcacc tctccacttc tggatataag gatattaggt
     3961 atggcaaccg ctggaatgct tctcaaactc aactctcaaa tgaaccgcga gttttacgca
     4021 tccaatctct accttcacct gagtaactgg tgttctgaac agagtctgaa cggcaccgcc
     4081 actttccttc gcgcccaggc acagagtaat gtgacccaaa tgatgcgcat gtttaacttt
     4141 atgaagagtg tcggcgctac ccccatcgtt aaagccattg atgttcccgg tgaaaaactg
     4201 aactctctgg aagaactgtt ccaaaaaacg atggaagaat acgagcaacg ttctagtacg
     4261 ttggcacagt tagccgatga agcgaaagaa ctgaatgatg attcaaccgt caatttcctg
     4321 cgcgatctgg aaaaagaaca gcagcatgat ggtctgttgc tgcaaaccat tcttgatgaa
     4381 gtgcgcagtg cgaaacttgc gggtatgtgc cctgtgcaga ccgaccaaca tgttctgaat
     4441 gtcgtgtcac accagctgca ttgatcatca tcggcgctaa tgcattgcgc cgatgaaggt
     4501 tttgagaaac cgctgcctca tctgtttgaa gcagcggttt ttttaatggg attcaccctg
     4561 tggggtaaac ttgagttcaa taagcgcgat ggctttttgg attgcccgca tggtgaccgg
     4621 gtctgcggcg gcgggatggt tagtaaagtc gatattcttc agctgactgg acattttttc
     4681 acgaacttca acgggcgcga ttacatcgag aacatccaga atttgtttga taaccaactg
     4741 gcaagcaacc acatcagaaa ccagttcctg atcggcattc agcggctggg acatcgtaaa
     4801 ctcctgatag cattttgaaa gccgttatag tagcggcttc acatcttcag cgatagtcac
     4861 atccaccgtc atcaggacac aaaaaaacct gccggagcag gttttttgtt atcggaacat
     4921 attgcctggc ggtacgtctt tgaacgtctt gcaatagtta ttgaacatac ttttcaggat
     4981 tttgcgcagt ttcatcgcgg cactccgacc atttgttata caggtgttat tgtctttgcg
     5041 cttataatat gacaaccatc acaaaaatca atctttatgt gatacaaatc acataaatac
     5101 ccctttaatg ttataaaaat gataatcaaa aaacagcccc cctatttctg acacctacag
     5161 atggcaagaa atagcgcctg ccaggcgtct tttccggcca ttgtcgcagc gctgtaacgc
     5221 gtaaaatagt gctttctctt actcttctgg ctggaccatg agacttctga ttctgactct
     5281 ttcattaata acgctggcgg ggtgtacggt tactcgccaa gcccatgtga gcgaagttga
     5341 cgcggcaaca ggtattgtac ggttggttta tgaccaggct tttttgcagc atgcccatac
     5401 cgatcgttat gtcagtcgcg ggatcgccga tcgcgcatgc cagcaggaag gctataccca
     5461 cgcggtcccc tttggtcagc cagtaggcaa ctgtagcctt tttgcgggtt ccttatgttt
     5521 gaatacggaa ttcactttgt catatcagtg ccatcattcc gccttccctg tctttctcta
     5581 aatattttaa tcagcgaggg gatcttcgct gattaaagaa atagcggtaa tgcgttttat
     5641 tcccattcgt atttttaata attgaagttt atattttacc ttttgcaaat aataaaataa
     5701 caaattatag tgacgccaca gcaacaaata taacctttgt ggagcactat catgctgaaa
     5761 ccagaaatga ttgaaaaact taatgagcag atgaacctgg aactgtactc ttcactgctt
     5821 tatcagcaaa tgagcgcctg gtgcagctat cataccttcg aaggtgctgc cgcgttcctg
     5881 cgccgtcacg cccaggaaga gatgacgcat atgcagcgtc tgtttgatta cctgactgat
     5941 accggcaatt taccgcgtat taataccgtt gaatctccgt ttgctgaata ttcctcactt
     6001 gatgaattat tccaggaaac ctataaacac gaacaattaa tcacccagaa aattaacgaa
     6061 ctggctcatg ctgcaatgac caatcaggac tacccaacat ttaatttcct gcagtggtat
     6121 gtttctgagc agcatgaaga agagaaactg ttcaaatcga ttattgataa attaagcctg
     6181 gcaggcaaaa gcggcgaagg tctgtatttt atcgacaaag aactctctac cctcgacaca
     6241 caaaactaat gctctccggc ggcaggttta ctgccgccgt ttcaaatcag tgacggcaaa
     6301 tcttactctg atcggtcgaa aaaccgtctt tcgaaggtat aaatttgcct tttgctgcca
     6361 gaaacgctac cagctctccc gctgtcatcc cttctgccga gcaggtgtga aaacgtgcct
     6421 gttcaccaaa acgcgcttta atcgcagctt ccagactggc atgcgtatat tgctcgcctg
     6481 attcaatcat catatttaac acttcatgac cgtgaataga gtccatcgtt cctcctcaaa
     6541 aaaagcctag cgtagcgatt gccgcttatg aagactttgc gccagcgcag gactgaatgc
     6601 tttttattgt acatttatat ttacactata tgtaacgtcg gtttgacgaa gcagccgtta
     6661 tgccttaacc tgcgccgcag atatcactca taaagatcgt caggacagaa gaaagcgtga
     6721 aaaacagaac cctgggaagt gtttttatcg tggcgggaac cacaattggc gcaggcatgc
     6781 tggcaatgcc gctggctgcg gccggtgttg gttttagcgt tacgttaatc ttgttgattg
     6841 ggctttgggc gttgatgtgc tacacggcgc tattactgct ggaggtgtac cagcatgttc
     6901 cggcagatac cggtctgggc acgctggcaa aacgctatct gggacgctac ggtcaatggc
     6961 tgacgggctt cagtatgatg ttcttaatgt atgctctgac tgcggcatac atcagcggtg
     7021 tcggtgaatt gttggcctcc agcatcagcg actggacagg tatttcaatg tcggcaaccg
     7081 ctggcgtgct gttgttcact tttgttgccg gtggcgtggt ttgtgttgga acatcgctgg
     7141 tcgatttatt taaccgtttt ctgttcagcg ccaaaattat ttttctggtg gtgatgctgg
     7201 tattgctgct accgcatatt cacaaagtga atcttttaac cctgccgttg caacaggggc
     7261 tggctctgtc agcaatcccg gtgattttta cctcatttgg ttttcacggt agcgtgccga
     7321 gtattgtcag ctatatggat ggcaacgttc gtaagctacg ctgggtgttt ataaccggta
     7381 gtgcgatccc cctggtggca tatattttct ggcaggtggc aacgcttggc agcattgatt
     7441 caacaacctt tatgggattg ctggctaatc atgctggatt aaacgggctg ttacaggcgt
     7501 tacgcgaaat ggtggcctct ccgcatgttg agctggcagt gcatttattt gctgatttag
     7561 ccctcgccac gtcatttctc ggcgttgcgt taggcttatt tgattatctg gctgatttat
     7621 ttcagcgttc aaataccgtt ggtggacggt tgcaaactgg tgcaattacc tttctgccgc
     7681 cgttggcgtt tgcactgttt tatccacgag gatttgtgat ggcgctgggt tacgccggtg
     7741 tggcgctggc ggtactggca ttgattatcc cttcgctgtt gacctggcaa agcagaaagc
     7801 acaatcctca ggcgggttac cgggtcaaag gtggtcgtcc ggcgctggtg gtggtgtttc
     7861 tctgtggtat tgctgtgatt ggcgtgcaat ttttgattgc ggcagggttg ttaccagaag
     7921 tggggtgatc agatagcctc aaattcctta ttgggtgcca gaattaacgc tgacacccaa
     7981 tttggcctct taatgcaggc agcactgctt aaatttctta ccactaccgc acgggcaagg
     8041 atcgttacgc cccggtttct cttctgcttt gatcggttgc tgaacagctt tttcctgcgg
     8101 atgcgccatc cagtacgcat gtagatcaag cgccgccagt cgaatggcat ctacgctctc
     8161 ttcaaacgct tctggcgaca tcttttctac ccgctcgaag ttttcctcag taccgtgcag
     8221 cgcaatcgcc tccagcgctg gttttaacga atcgggcaac gttgaccagt cagaaagtgc
     8281 cacgccccgc atatagccaa agcaccactc ctcaacaatc gtcagctcgc tgccatcaac
     8341 ttctcgcaag ccgaataacg gctcaaactg ctccgggaat tcgttcagac gctctgcggt
     8401 atcggccata tgttgaaaag ccaggttcat aaagcgcgtc atctctttct ctgacgccca
     8461 gcgcggcaca tagtcagccc caccccacac ggcaaccagc cactgttccg gttcaatctc
     8521 ttgcggagaa ctcaacaccg ccgtcaataa accgtccagc tccgccacat caaggatggc
     8581 gtggttagtg ttgtatttgg tcagaatatc gtccagccat tccaactcac tttcgtttaa
     8641 cggtcccgtt ttcatacgct tttccttgtg gatctcaact cgccagcacc tatcttacat
     8701 gccggtccgt atcagagata ctttttgagt ggctttgccg gtgattaaaa attaaggagg
     8761 gtgtaacgac aagttgcagg cacaaaaaaa ccacccgaag gtggtttcac gacactgctt
     8821 attgctttga ttttattctt atctttccca tggtacccgg agcgggactt gaacccgcac
     8881 agcgcgaacg ccgagggatt ttaaatccct tgtgtctacc gattccacca tccgggctcg
     8941 ggaagaaagt ggaggcgcgt tccggagtcg aaccggacta gacggatttg caatccgcta
     9001 cataaccgct ttgttaacgc gccaaattct tcaggccttt cagccagaca tccgcttgac
     9061 gccgatgtct tttaaactgg agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc
     9121 aaggtcgtgc tctaccaact gagctattcc cgcattcatc aagcaatcag ttaatcactt
     9181 gattttatta tcgtctggca atcagtgccg ccgttcgatg cgttgcattc tacttacctg
     9241 gcgcgatgag tcaacgatat ttttcaccac ttttgatcgt ttgctgaaaa ttacgccgaa
     9301 acgatcactg atcaagcaaa tctgcacgcg cagcgctcaa atattgcaac attgaccaca
     9361 gagtcagtac cgcagccaca aagaaaagtg caataccggc gtactcaacc caaatgttcg
     9421 gacgccacag cagccatgcc aacgccacca tctgggcagt ggttttcact ttcccaatcc
     9481 aggagacagc cacgctactg cgtttaccca actccgccat ccattcgcgt agcgcagaaa
     9541 taataatttc acgggcgatc atcgttgctg ccggtaaggt cacccaccag ctgtggtaat
     9601 gctcggttac cagcaccatg gcgatagcca cgagaacttt atctgctaca gggtcaagga
     9661 aagcaccaaa ccgggtactc tggttccagc ggcgtgccag aaaaccatcg aaccagtcag
     9721 tcaccgccgc gacgcagaaa atgagcgcgg cggcaaacgg cgaccaggtg acaggcagat
     9781 aaaagaccaa tacaaagaat gggataagga tgacacggaa cagtgtaagc aacgtaggga
     9841 tattaaattg cataatgacg ggtaactatc tgttgtcagt aagattaccc ctatgttgct
     9901 acagagacat caatgtttca acgaccagaa gatcttttct gccagacctt gcgaaatacc
     9961 cggcactttt gcaatttcct cgacgctggc gttacgtaaa ccttgcaaac cgcccatata
    10021 tttcaacaac atttgccgac gttttggccc gacgccttca atggtttcca gggaactggt
    10081 atttttgacc ttcgcccgtt ttttacggtg cccgccaatc gcgtgatcat gtgattcatc
    10141 gcgaatatgc tggataacat gcagcgcggg agaatctggc ggcaaactga atccctcacc
    10201 ttccggctca aagaacagcg tttccagccc agccttacga tctgctcctt tggcaacgcc
    10261 aagtagcagc ggatgatttt tatcccatga gacatccagt tcggcgaaga catttttcgc
    10321 ctgcgcaagc tggcctttgc cgccgtcgat aaggatcaca tccgggatct tactgtcgtc
    10381 aatggcttta ccataacgcc gacgcagcac ctgattcatc gccgcataat catcgcccgg
    10441 cgtgatgcct gtaatgttat agcgccgata ctccgcacgc agcgggccgt tagcatcaaa
    10501 caccacacag gaagcgacgg tttgttcgcc catggtatgg ctgatgtcaa agcactccat
    10561 ccgcttcact tccggcaatt tcaacacgct ggcaagcgcg gtcagtcgct ggtgaacggt
    10621 agattgctgc gaaagtttgc tggttaaggc cgtcgccgca ttggtgcgcg cgagtttcag
    10681 ataacgcgcc ctatcgccgc gaggtttggt ttgaacatta atcttgcgtc ccgccagttc
    10741 tgaaagggaa tcggcgagca gcgttttatc gctaagatta aaatcgagca ggatctcacc
    10801 cggtaaggtg cgcatctggc tgccttgtaa atagaactgg cctacgaagg tttctaccac
    10861 ctcgctcagt tccgtaccgc caggcacttt cgggaaatag ctgcggctgc cgagcacttt
    10921 gccctgacga atgaacaata cgtggacaca agccatgccc gcatcgaacg ccacaccaat
    10981 aacgtcgagg tcgtcgccgg tattggaaac gaattgtttt tcggtgacgc gtcgcaccgc
    11041 ctgaatttgg tcgcgaatac gtgcagcttc ttcaaactcc agattctggc tggcagtttc
    11101 catacgacta atgagttgcg taagcacctg atcatctttg ccagacaaaa acaggcgcac
    11161 atactcaacc tgctgagcgt attcttcttc actcaccagt ccttcaacgc acggtcccag
    11221 acagcgccct atctgatatt gcagacacgg acgcgagcga ttgcgataaa cactattttc
    11281 gcactggcga atggggaaaa tcttttgcag tagcgccagt gtttcacgta cggcatagcc
    11341 attcgggaac gggccgaaat attcaccttt ggcatgcttc gcaccacgat gcatcgccag
    11401 acgcgggtgg gtatcgccac tcaggaagat aaaaggataa gatttatcat cgcgtagcaa
    11461 aacgttgtaa cgcggctgat agagtttgat gtagttgtgt tccagcaaca gcgcttcggt
    11521 ttctgtatgc gtaaccgtta catcaatttg ctggatctgg gcgaccagcg cttcggtttt
    11581 gcgcgaagcg aggttgctgc ggaaatagct ggaaagccgt tttttcaggt ctttcgcttt
    11641 gccgacatag ataaccgtac caccagcatc gtacatgcga taaacgcctg gctggcaggt
    11701 tacggttttt aaaaacgctt ttgcgtcaaa ctgatcactc actgacttga taatgtctcc
    11761 gcattacaca gaccatggcg aattgccagg tgagtcagct caacatcgcc atgaatgttt
    11821 agtttactga acatacgata gcggtagctg ttcaccgttt tcggactgag attgagctgt
    11881 tctgagatct cattgacctt ctggcccttg gtgatcatca gcataatctg caattcacgt
    11941 tcagacaaac tggcaaatgg gctttctgtt ttttctggtt cgatctggct taacgccatt
    12001 tgttgagcga tatcagaagc aatgtaacgc tgccctgaat agacagaacg aatcgcactc
    12061 acgacttcct gcggagccgc gcctttgctg aggtagcccg cagcaccggc ctgcatgact
    12121 ttcgctggta aagggttttc tgtatggacg gtaagcatga tgattttgac atcagctgtg
    12181 gaacgcgcga ttttacgcgt cgcctcaaga ccgccaatgc ccggcatact catgtccatt
    12241 agcaccacgt caacggcatt tgtccggcac cacttaacgg cgtcttcacc gcacgatgcc
    12301 tcaccgacga cttttatacc ctttatatct tccagaatgc gtcgtatccc tgcgcgcacc
    12361 agttcgtggt catcaacaag tagaacgttg atcaaaggaa tatctccaga aatagggata
    12421 acgctactga tagttagtca ttcgtatatt agcggttttt attgcaactt tgaaacgtta
    12481 aaaatggtgg gtattcgatt tttctctcgt ttttgtaaat tcgattgtcc acagttatgg
    12541 tcacgcccgt caccaacggc ttccgggatc gtttcacagt aagcccggcg atgaaatgtg
    12601 acaaaaattg actttattca gcaaaaatga aaatcagccc gctgaatatg taacattaat
    12661 taacccgaac aatacgggca aaaaacattc atgataaaaa tatttattgg tcattatatt
    12721 aacgcattta atagcattgt tgacgtcacg ctcaaaaaac aaccactgct atttttagca
    12781 aagcttatgg tatactccgc cgccttaaca tttttcaccg caaattttca ttgcaacatg
    12841 acgaggaaaa taaatgagta cgcctgattt ttctactgcc gagaataatc aagaactggc
    12901 aaatgaagtc tcctgcctga aagcgatgct gacgctgatg ctacaggcga tgggacaagc
    12961 tgacgcgggc cgcgtgatgt taaagatgga aaaacagctt gcgctgatcg aagacgaaac
    13021 ccaggctgca gtattttcca aaacggttaa gcaaattaaa caagcctacc gtcagtaata
    13081 taaaaccggc tgagagcgtg cctttcagcc gatttttgca tctggcacgc agaaagaaaa
    13141 gcgatcaaat taagccagta gcggccgcgt aacaggccac ctgggtctta tttggtgcat
    13201 tgattttttt ctgcatgttt ttctgatgga aattgaccgt attctcagag attgacaaaa
    13261 tcatcgctat ctctgctgat gtcttccctt ccgccgtcca tttcagaatt tctttttcgc
    13321 gcttgctgaa attcatctct ggcgtcatca ctatttcatc atttaaacgc atcagagcca
    13381 tcagactttc gcgcaccagt aactgcattt ttaattgcag ttcatcacta agaatgggta
    13441 tttcgcgcgt gctgcaacgg gaaaaggaca aaaagcccag cgcccgattt ggcagcatta
    13501 aatactgagt gacaccgcgg cgtaaaccat gtgcgtgcgc ggcttcccat aatggctgtg
    13561 cttcgctgaa taagtcatca ttccacatta aatggccctg actaaagttt tcagggttca
    13621 gcacaggatc aattgcgaga aaattttttg cctgataata actaacccac gactcagggt
    13681 aattggtgta gaaagccact ttaggtcgcg tgaatggtac cgggtggcga acacataacg
    13741 aatagtaatc gtactccagc tgctgagcct gaagctctat ttcatggtag acctcttctg
    13801 cggcctccat cctctgaaaa cgcaacagca tcgtgcgacg ccagctgaaa aaatccgtat
    13861 cctgcatagt aaaccgcaac gcccctgaga gtgagtatca tttataatga taattcaaac
    13921 tatcacataa ataagattta tatataattt atattattca ggcaatgaat tacttttgca
    13981 agccatcgca ttctcttatg ttattaatga gttatgctga tttgttaagc agttttatca
    14041 cgcttgaaat ggcgtcccgc cccgacaggt gaatcgtcgg ggctgatttt ttcttattat
    14101 tgcagtagaa acttctcgag gaactggcgg gtgcgaggct gctggggatc agcaaataac
    14161 gcttttgctg ccccctgctc gactatccgc ccctggtcca taaaaatcgc ccggtccgca
    14221 acatcccggg caaagctcat ttcgtgcgtc acaatcacca tcgtgcgctt ttcctgcgcc
    14281 agctggcgga ttgtgttcag gacttcgccc accagctctg gatccagcgc tgacgttggc
    14341 tcgtcaaaca aaatcacctc aggacgcatt gccagcgcac gcgcaatcgc tacacgctgc
    14401 tgttgaccgc cagacaaacg acgtggatag ctggtttctt taccggccag cccaactttc
    14461 gccagcagct cgcgagcgcg cgccgtggcc tcttctttcg gttcgccttt gacgatcacc
    14521 ggcccttcaa taatgttttc cagcaccgta cgatgcggaa acaaattaaa gttctggaag
    14581 acaaacccga cgtgctgacg caactggcgg atcagcgatt tttgctgact taatgaacgt
    14641 gcagtatcaa tagtgatatc gccaacggtg atcgttcccg cttccggttg ttccagcaga
    14701 tttatgctgc gtagcaacgt ggttttgccg gaaccactcg gaccgataat tgccaccact
    14761 tcgccaggct ttacctcgag gtcgataccg tgcagcaccg tctgaccgtg aaattttttc
    14821 accaggttct taacttcaat ggcactcatt ttggttctct ctcctggcga ttaagttgat
    14881 tctcaaaatg gttctgcaac gtcgataata ctgtcgccat gatccagtag atgagcgaag
    14941 ccgccaggta catggtgaac acctccagcg tgcgcgaggt aatcaactgc gcctgacgga
    15001 acagctccgg cacctggatt gtcgcggcca gcgacgtatc ctttaccagg ctgataaagc
    15061 tgttcgacag cggcggcaac gccacccgcg ccgcctgtgg caaaatcgcg cgacgcatag
    15121 tctgccatgg cgtcatacca atactggcag ctgcttccca ctgaccttta tcaatagaag
    15181 aaatggcggc gcgcagtgtt tcggcggcat aggcggcagt gttcagcgac agaccaatca
    15241 ttgctgacgg aatgggatct aattcgatac caaactgcgg caggccgtaa tagatcataa
    15301 acagttgtgc gatgagcggt gtaccacgaa aaatagagat ataaaaacgc gccagccagc
    15361 gcacgggcca gattggcgac aggcgcatca gcgcgagaat aaacccgagc agtaagccaa
    15421 aaaacatgcc gccgatactg agttgcagcg tatacccagc ccctttcagc aggaacggca
    15481 aggaatcaat aaccagttgt atactttctt gcatgagcgt tcccggagct aaacgtgggg
    15541 atgataggcg aacagcgcag gcgcaccgcc ggtatgaata aacagaatcg gcccttcatc
    15601 tttgaagcgt ttctgactga tacagccaat cagccccgcc atcgcttttc cggtatacac
    15661 aggatcaagc agaatgcctt ccagccgcgc cagcaatttc actgcttcca tgccttcgtc
    15721 gttcggcacg ccgtagccag gtgcaaaata gtcatcccag agtataattt ccgctgatgc
    15781 ggtcagctcc agttctttcg caatcgcctg ttgtaggtta accactttcg gcaactgatc
    15841 ggcaacggaa cgcgacacgg tcacgccaat cagttcgctt tcaggcatca ggtgttccag
    15901 cccaacagcc agtccggcgt gagttccggc actgcccgat gcgactacca ccgacgaaat
    15961 attaaccgcc ccttcacact gttgcgcgat ttccagcgca ctctccacat aacctagcgc
    16021 gccaagagca ttagaaccgc caaccggaat gacatacggg cgaaagcctt gtgcttcgac
    16081 tcgcgtcgcc agctcttcca gttgggcatt gggatcggtc agtgcttcgc acatttcaat
    16141 ctgggtattg aacagatcca gcaacaagcg attgccgttg gttaaatagt tttctgcggt
    16201 tgtgccaata ggattttcca gcagcgccac acagtgcaga ccgagtttcg ccgcaactgc
    16261 ggcagtctgc cgcacatggt tagactggat caccccggca gtaatcagcg tatcggcacc
    16321 ttcgcgcaga gcatctgccg cgagaaattc tagcttacgt aatttattgc cgcccattgc
    16381 catgggggtg acgtcatccc gtttgatgaa aatttcccgt cctagataat cagaaaagcg
    16441 cggcagatat tcgagcggcg ttggcgcgcc gataaactcc aaccgtggaa aacgggttaa
    16501 attatgcagt ggcataacag cctccgatgt atgttgttgt gattttctta ttatgcacgc
    16561 tgaaaacgcg taaataaaaa agg
//
LOCUS       DAJVRF010000086        16554 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJVRF010000086 DAJVRF010000000
VERSION     DAJVRF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16554
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.086"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="OQN13_002585"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="OQN13_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCS1615910.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="OQN13_002586"
     CDS             433..621
                     /locus_tag="OQN13_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCS1615911.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="OQN13_002587"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="OQN13_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCS1615912.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="OQN13_002588"
     CDS             816..989
                     /locus_tag="OQN13_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCS1615913.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="OQN13_002589"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="OQN13_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="OQN13_002590"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="OQN13_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCS1615914.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCLSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="OQN13_002591"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="OQN13_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCS1615915.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="OQN13_002592"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="OQN13_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCS1615916.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="OQN13_002593"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="OQN13_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCS1615917.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="OQN13_002594"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="OQN13_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="OQN13_002595"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="OQN13_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCS1615918.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="OQN13_002596"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="OQN13_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCS1615919.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            12531..13677
                     /locus_tag="OQN13_002597"
                     /pseudo
     CDS             12531..13677
                     /locus_tag="OQN13_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13971..15212
                     /gene="agp"
                     /locus_tag="OQN13_002598"
     CDS             13971..15212
                     /gene="agp"
                     /locus_tag="OQN13_002598"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCS1615920.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(15250..15477)
                     /locus_tag="OQN13_002599"
     CDS             complement(15250..15477)
                     /locus_tag="OQN13_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCS1615921.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(15498..16103)
                     /gene="wrbA"
                     /locus_tag="OQN13_002600"
     CDS             complement(15498..16103)
                     /gene="wrbA"
                     /locus_tag="OQN13_002600"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCS1615922.1"
                     /translation="MSVKARILVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPE
                     TMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWA
                     SGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGG
                     TPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            16155..>16554
                     /locus_tag="OQN13_002601"
     CDS             16155..>16554
                     /locus_tag="OQN13_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1615923.1"
                     /translation="MKLLVTYIRWSTKEQDSGDSLRRQTNLIDAFYSKHKNDYYLLPA
                     HRYVDKGKSGFHQQHKNQGSDFRRMFENVMSGAIPEGSLIVVENFDRFSRADIDTAID
                     DVRQILRKGVSILTLGDGELYDKSALTDPVK"
BASE COUNT         4159 a         4071 c         4226 g         4098 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgcggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggt gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcaag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gcggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggcat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgcgtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatcgc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtttaa ctagggcgtc attatttatt aaattttata gacgctatat atgggtagta
    12541 atatacatgg aattagttgc actgcaaata attatttgaa acaggcctgg aacgatataa
    12601 aaaatgagta cgaaaaaaat caaacatatt caatcacgct ttttgaaaac acactggtgt
    12661 gttttatgcg gttatacaat gaactcagac gtaaagtaaa tgaagaggat actccatgtc
    12721 tggaatgtga atcactagaa aaagaatttg aggaaatgca gaatgataat gatctatcat
    12781 tatttatgag aacattgcgt actaatgata cacaaattta ttcaggggtt tcaggaggta
    12841 ttacatatac tatacaatat gttcaaaatg ttgatattgt tagagtgtcc ttgccgggca
    12901 gaggttcaga gtctatcaca gattttaaag gttattattg gtatggcttt atggaatata
    12961 ttgaaaacat tactgcgtgt gatgacattt tttctgagta ttgtttagat gatgaaaata
    13021 tgagtatcca gccagaacag ataaatacgc cgggaatatc tgatttagat actggtttta
    13081 aaagaacatg agtattcatg ggcgctgggc ggccataaga cgcaaaagca agtatacgaa
    13141 gagtttatta aaattgttga taaacaaacg atcgaggctc ttgttgatac ggcattctat
    13201 aatctcaggg aagatgtaaa gacgttattt ggcgttaatc tacaatctga caacaaaatt
    13261 caggggcagg gtggtttttt atcatggagt tttttgtctt ttaaaaaaga atttattgat
    13321 agttgcttga atgacaaaaa atgcatactg catttacccg aatttatatt taatgataac
    13381 aaggcacgcc ttgttttaga caccgaccca gagcagaagg ttaatgaggt gaaaaatttc
    13441 ctcactgctc tttcagatag catttgctca ttatttattg tttacagcaa tgtggcatca
    13501 atctccttag ggaatgaatc cttttcaaca gatgacgacc ttgagtatgg gtatttaata
    13561 aacactggca atcattatga cgtttacctc cctcctgaac tttttgctca ggcttacgag
    13621 ttaaacaata aggaaaggaa tgcgcaaatt gattttttaa ctcgttatgc aatttaatga
    13681 caaaggcatg tgctaaaaac cattgttatt agcctcactt ctcttattat tggtactatt
    13741 gcctctgtat tagtaacgcc gctgatattc tgtttatcca caggtgcaat tatcagcggc
    13801 gtacgcgagg caggggctaa tcaggcatag tttgcgtcaa accttgcctg tttttgaaga
    13861 tgtatataga aaaacaggcg ttcaacaagc cattttgcga acctgttccc ggaaaaaagt
    13921 catatttctg tcacactctt taatgattga taacaaaaga ggtgccagga atgaacaaaa
    13981 cgctaatcgc cgcaactgtg gcagggatag ttttactcgc ttcaaatgct caggcacaaa
    14041 ccgtaccgga aggctatcag ctacagcaag tgctcatgat gagccgccat aacttacgtg
    14101 cgccgctggc gaacaatggc agtgtgctgg agcagtcgac gccgaataaa tggccagaat
    14161 gggacgtccc cggtgggcaa ctcaccacca aaggtggcgt gctcgaagtg tatatgggcc
    14221 attacatgcg tgaatggctg gcagagcagg ggatggtgaa atcgggggaa tgcccgccgc
    14281 cggacaccgt ttatgcctat gccaatagtc tgcaacgtac cgttgcgacc gcacagttct
    14341 ttattaccgg cgcattcccg gggtgtgata ttcctgtgca tcaccaggaa aaaatgggca
    14401 ctatggaccc aacctttaac ccggtgatca ccgatgattc cgccgcattc agtgaacagg
    14461 cggtggcggc aatggagaaa gagctcagca aactccagct taccgacagc taccagctac
    14521 tggaaaaaat cgttaactat aaagattccc ctgcctgtaa agagaaacaa cagtgttcgc
    14581 tggtggatgg caaaaatacc tttagcgcca agtatcaaca agaaccaggt gtttccgggc
    14641 cgctgaaagt cggcaactcg ctggtagatg cgtttacttt gcaatattac gaaggttttc
    14701 cgatggatca ggtggcctgg ggagaaatca aatctgacca gcagtggaag gtgttgtcga
    14761 agcttaaaaa cggctaccag gacagcctgt ttacctcacc ggaagtggcg cgcaatgttg
    14821 cgaaaccgct ggtcagttat atcgacaaag ctctggtcac cgatcgcacc agcgcaccga
    14881 aaattacagt gttggttggg cacgactcca acattgcctc tctgttaacg gcgctggatt
    14941 tcaaaccgta tcagttgcat gaccagaacg aacgcacgcc gattggcggc aaaatcgttt
    15001 tccagcgttg gcatgacagc aaagccaatc gcgatttgat gaaaattgaa tatgtgtatc
    15061 agagtgcgga acagttacgt aatgccgatg cgttaaccct gcaggcacct gctcagcgtg
    15121 tgacgctgga attaagcggt tgcccgatag atgccaacgg tttctgcccg atggataaat
    15181 ttgatagcgt gttgaatgaa gcggtgaaat aacagaaaac tcccccgcga gaagcggggg
    15241 agtcgctggt taaacgtttt tacgttcgat ggtctgttcg ccccaaaaaa gcgaatcttt
    15301 atcggcctta gcaaaggctt tgactaacac ttcatcgcta ccttcttccc aaatcttttc
    15361 tgccattttt tcgtcgtacc cggcgacttc gaaaatggcc tcggctattt ccggcgacgt
    15421 attgcgcaga gatgcccatt caccgacgtg atgagctttc gcttcttgag ttggcatgcg
    15481 tatcctcctg ttgaagatta gccgttaagt ttaactgcca gacctgcgac atattcccct
    15541 tgataacgag caatagacag ttcttcctgg cttggctggc gtgagccgtc accgcctgcg
    15601 atggtggttg cgccgtacgg cgtaccgccg cgaacctgtg aaacgtcaaa taattcctgc
    15661 gctgcgtagc caatggggac aatcaccatg ccgtgatgcg caagggtcgt ccaggttgaa
    15721 gtaatagttt gttcctgacc gccgccagta ccggtggaac taaagacgct cgccagtttt
    15781 ccgtatagtg cgccggaagc ccacaggccg cccgtctggt cgaggaaggt acgcatttga
    15841 ccggacatgt tgccaaagcg ggtaggtgta ccaaaaataa tggcgtcgta atcggccagt
    15901 tcttgcgggg ttgcaaccgg tgcagtttgc gttttaccgc ctgctttttc aaataattgc
    15961 ggcggcatgg tttccggtac acgcttaacg acaacttctg cgccatcgac tttgcttgca
    16021 ccctcagcga ctgcgcgtgc catcgtttca atatgtccgt acatggaata ataaagcact
    16081 agtattcttg ccttaacact catatcattg ttctcctgtt caatgaacca cttagataag
    16141 catagtccga agatatgaag ctgctagtga catacatcag gtggagtact aaagagcaag
    16201 actcaggaga ctccttaaga cggcaaacaa acctcattga tgctttctac tctaagcaca
    16261 aaaacgatta ttaccttctt cctgctcaca gatatgttga taagggtaaa tctggttttc
    16321 accagcaaca taagaatcaa ggttctgact tcagacgtat gtttgaaaat gttatgtctg
    16381 gggctattcc agaaggttcc ttgattgttg tagagaactt tgaccgcttc agtagggctg
    16441 atatcgacac agctatagat gatgtcaggc agatactgag gaagggggtc tctatactga
    16501 ctctgggtga tggtgaactc tacgacaagt cagccttaac agaccctgtg aagt
//
LOCUS       DAJVRF010000087        16458 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJVRF010000087 DAJVRF010000000
VERSION     DAJVRF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16458
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.087"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..794
                     /locus_tag="OQN13_002602"
     CDS             <1..794
                     /locus_tag="OQN13_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCS1615924.1"
                     /translation="IASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLHTDYLD
                     LYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFG
                     VMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTG
                     KYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPF
                     VASTLLGATTMDQLKTNIESLHLELSEDVLAEIEAVHQVYTYPAP"
     gene            complement(826..2019)
                     /gene="lplT"
                     /locus_tag="OQN13_002603"
     CDS             complement(826..2019)
                     /gene="lplT"
                     /locus_tag="OQN13_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCS1615925.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETMSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2012..4171)
                     /gene="aas"
                     /locus_tag="OQN13_002604"
     CDS             complement(2012..4171)
                     /gene="aas"
                     /locus_tag="OQN13_002604"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCS1615926.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            4757..5788
                     /gene="galR"
                     /locus_tag="OQN13_002605"
     CDS             4757..5788
                     /gene="galR"
                     /locus_tag="OQN13_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCS1615927.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(5795..7057)
                     /gene="lysA"
                     /locus_tag="OQN13_002606"
     CDS             complement(5795..7057)
                     /gene="lysA"
                     /locus_tag="OQN13_002606"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCS1615928.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7179..8114
                     /gene="lysR"
                     /locus_tag="OQN13_002607"
     CDS             7179..8114
                     /gene="lysR"
                     /locus_tag="OQN13_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCS1615929.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8101..8748)
                     /locus_tag="OQN13_002608"
                     /pseudo
     CDS             complement(8101..8748)
                     /locus_tag="OQN13_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase"
     gene            complement(8879..10297)
                     /gene="araE"
                     /locus_tag="OQN13_002609"
     CDS             complement(8879..10297)
                     /gene="araE"
                     /locus_tag="OQN13_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCS1615930.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10612..11373)
                     /gene="kduD"
                     /locus_tag="OQN13_002610"
     CDS             complement(10612..11373)
                     /gene="kduD"
                     /locus_tag="OQN13_002610"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCS1615931.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11438..12274)
                     /gene="kduI"
                     /locus_tag="OQN13_002611"
     CDS             complement(11438..12274)
                     /gene="kduI"
                     /locus_tag="OQN13_002611"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCS1615932.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12561..13742)
                     /locus_tag="OQN13_002612"
     CDS             complement(12561..13742)
                     /locus_tag="OQN13_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCS1615933.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            13997..15226
                     /locus_tag="OQN13_002613"
     CDS             13997..15226
                     /locus_tag="OQN13_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCS1615934.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            15686..16318
                     /locus_tag="OQN13_002614"
     CDS             15686..16318
                     /locus_tag="OQN13_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1615935.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT         4340 a         4317 c         3811 g         3990 t
ORIGIN      
        1 ttatcgcctc caaagtgagc ggaccgtcgc gcaataatga caagggcatc cgcccggatc
       61 aggcgctgga tcggaagaat atccgcgaag cgctgcatga cagcctcaag cgcctacaca
      121 ctgattacct cgatctttat caggtgcact ggccgcagcg cccgaccaac tgcttcggca
      181 aactcggtta tagctggacg gattctgcgc ctgcggtttc gctgctggat acgctggacg
      241 cactggcaga gtaccaacgc gcgggaaaaa ttcgttatat cggcgtgtcg aacgaaactg
      301 catttggcgt aatgcgctac ctgcatctgg cggacaaaca cgatctgccg cgtattgtca
      361 ccattcagaa cccttacagt ctgttaaacc gcagttttga agtaggtctg gcagaagtaa
      421 gccagtatga aggggtcgaa ctgctggcct attcgtgcct aggtttcggc acgctgaccg
      481 ggaaatatct caatggtgca aaacccgctg gcgcacgtaa tacgctcttt agtcggttca
      541 cccgctatag cggtgagcaa acgcaaaaag ccgtcgcggc gtatgttgat atcgccagac
      601 gtcatggcct agaccctgct cagatggcgc tcgcgtttgt acgccgtcaa ccgtttgttg
      661 ccagcactct gctgggcgca accacgatgg atcagctgaa aactaacatc gaaagtttgc
      721 atctggagtt aagcgaagac gtattagctg aaattgaagc ggtgcatcag gtttatactt
      781 atccggcacc ataagattaa cgccggttaa aaccggcgtt aaatattaat gacggcgctg
      841 ccagatccac agcgccgtta ttgccagcgc aaacagcgca ccgaagccaa tgccaatggg
      901 cacgaccggg atgcctacca ttaccgccag cgagtaaatg cccagcatca acaacatggc
      961 gctgttttcg ccaaggtttt gtactgcaat cgcattcccc gccccgacgc tttttttacc
     1021 ccgctcctgt agcaacgcat tgagcggaac gacaaaaaag ccccccatca cgccaatcag
     1081 catcaacaag gcataggctg gcagcagctc gtgttgcagg gaaaaaatca gtaccaccac
     1141 gccaatcaaa atcccggctg gcatacagcg tgacatggtt tccagcgtaa ctaacttcgc
     1201 tgccgcacct gcgccaacca cgataccaat cgctaccatc gcgttgagat aggtgggcgt
     1261 agcgttatcg gtaatgccca gcgccaccgg tacccacagc accaacagga aacgcagcgt
     1321 gacacccgct ccccagaata aactggtgcc caccagcgaa aaacgcgttt caccattgcg
     1381 ccatagcgag gtgcaggcat tcaggaaact gcgggtcatg ttgatgagat tccaggactg
     1441 ccccggacgc gccgccgcca gtttgggaat gtagatattg gcaacgaccg caccaccgta
     1501 ggccagtgcg catgcggcca gggcgacgag gacatgccag tcagccagca caccaccggc
     1561 tacggaaccg agcaaaatcg ccgctatggt agaagcttcc attaaaccgt tagctttcac
     1621 taacttacta cccgtggtta attcgccgag aataccgtat ttcgccggtg aataggctgc
     1681 agcaccaaca cccaccagcg tatagccgag aaacggattg ataccaaagc agatactggc
     1741 tgcgcccagc agcttcaggc cgttggcaaa catcatcacc cggcctttgg cgaagctatc
     1801 cgccacctgc ccgacaaacg gcgcaaaaag aatgtaagca cctacaaaca ccatttgcag
     1861 gatgggctgg ctccactccg gatagaactg cgctttcagt aacgccagag tggcaaacag
     1921 tagggcatta tcgccaaacg cagagagaaa ctgcgccacg ataaccgctt tcatcccctt
     1981 cgaccacaac gaagtgttag tgtgcactga ctcactcatc gtgttgttcc gcttcgtcta
     2041 cccagctttt caacgtgaca aagtcaggtt tgccgctgcc aagtaatggc atctgtttca
     2101 gatagcgaat atcgcgcggt acagcaagct ccggtacgcc gtgctcgcgg gcatactgtt
     2161 gcaacttatc gcgcgtcagt tcgttatctg tggtgaaaag caccagtgcc tcgcctttgc
     2221 tggcatcgct cttaatcgca gtggcatgga ctttatctgg cgaaacacca agtgccagtt
     2281 gttccaccat ttccagcgac accatttcgc ctgcaatttt ggcaaagcgt tttgcgcggc
     2341 cctgaatctg cacaaagccc tgctcgtcaa aacgcacaat atcgccagtg tcataccagc
     2401 cgcgctccat ttcgccgcga acattctcgg cggtgggcac ttccagtaca cctggcttct
     2461 ccacccgcag atagccgttc attatgttcg gccctttcag ttgcaggcgt ccgccctctt
     2521 cgataccagg gaccgacaac aggcgcgcat ccattcctgg tagaatacgc cctaccgtac
     2581 cgggtttcgc cgccatcggt acgttgatag aaacgacagg cgcgcattcg gtcacgccgt
     2641 agccttcaag gatgcgcagg ccaaatttat cctgccaaag ctgtttggta ctttcttgta
     2701 atttttctgc gcctgccacc acatagcgta gacgatagaa gtcatacggg ttggcgaaac
     2761 gcgcgtagtg accgaggaaa gtcgaggtgc cgaacaacac ggtgcaactg cggtcataca
     2821 ccaactccgg cacaatacgg taatgcagcg ggcttggata aagaaacact tctgcacctg
     2881 tgagcagtgg cgtaaacagg cctaccgtca gcccaaagga gtgaaacagc ggtaacgccg
     2941 acataaagcg atcgttggtg gtgaagtcgg caatcgtttt aatctgctcg acgttcgcca
     3001 gaatgctttt atggctatgg acgacgcctt tcggatggcc ttcagaaccg gaggtaaaga
     3061 ggatcagcgc ctcttcttcc ggctgctgtt ttacctgtgc cagacgtggc atcagcagat
     3121 gcgcgaagat ccatactttg tcggcagtgg tgacatctgc ttttaaatct tccagataga
     3181 cccagcgcac ctgagtaagt tgctccggca gatgccagag tttgccttta tcgagaaact
     3241 ggcgggaagt gaagatggtt ttgatttcag ccgccgtaat agcactggtc agccctttta
     3301 ccccggcagt gtagttcatc attgcgggaa tgcggcgacg ggcgatggcc ccaaaaatca
     3361 ctgccgcact gatgcctgca ttgggcagca ttaagccgat gcgttcgcct tcaacactgt
     3421 atttttcaag gatgcgtcca acaaacagcg ttttcgtaag caatttgcga taggagtctg
     3481 gggtaaagtt gacgtcttcg acacatttct tcccggctcc gaagcggtac attgcactca
     3541 gtaaagattc gtacagcgtt tcacgcgggc gcaccgccat tcgcgcttcc atcattattt
     3601 gatgcagcat ttcgccagcg attttgcgac ggtcacgggc acgcggcgca tccggcatct
     3661 ccacctgcgt tggtggcaaa atatgcagag taatttgcgg gaacaagcga cgtttaacca
     3721 gacctttcag gcggctgaag tgcgtaagtt ccgccccttc aatacgcaca ggaataaccg
     3781 ttgcaccaga cttcgccgcg acaaaacccg cgccatcgta gattttcatc agcgagcctg
     3841 tggtggtgat gcgtccttca gggaaaatca ccactggtcg gccctgttcc accagacgta
     3901 ccagatgttt aatcgccata ggttgcgtcg ggtcgagagg aacaaagtcg ataaatgatt
     3961 tcagccaacg catataccac tgttggctta ttgaggtgta aacggcaaac actggacgca
     4021 caggtaaaaa cagtccaagc aaaatgccat caataaaaga gacgtgatta ggcgtaatta
     4081 gaacgcgctc gcccttcagt gcctgggtgt cacccgtaac gcgaacgcga tacaaaacac
     4141 ggcacaaatt tcgaaaaaag ctaaaaagca ttccaactcc ctttgctctg attcagtaaa
     4201 agcgaatgga gggagattac acgagataaa gaacgcgagc gacagtaaat taggtgcgaa
     4261 aaaaaacctg cgcatccgcg caggttggtg caagagacag ggtacgaaga gcgtaccgaa
     4321 taatctcacc aatcaatacc tctgggatct tgattgtggt ctgcacgacg actcttcgcc
     4381 agcgagaaaa cgcaaaggaa tgaagggaaa tgcaacgagg tgtgtaaatt gtcggttact
     4441 gttacagatt gatgaccggc aaaaaaaaac ctgcgcatct gcgcaggctg gtgtaattca
     4501 tgtgctcaac ccgaagttga cttcacctat caatacctct gggatcacca ctttagcaac
     4561 ctgaagccaa acgccaccag cagtcaatcg caacagcatt tcgcaaagtg taaccaaagg
     4621 tttgcaatct ctttttttgt cttacttatt tgcgtttggc tcacattccc acgatgaaaa
     4681 cacgccgccc cttgaaccaa cgggcgtttt ccgtaacact gaaacaatgt aagcgtttac
     4741 ccactaaggt attttcatgg cgaccataaa ggatgtagcc cgactggcag gcgtttcagt
     4801 cgccaccgtt tcccgcgtca ttaataattc acccaaagcc agcgaagcgt cccggctggc
     4861 tgtgcatagt gcaatggagt ctcttagcta tcacccgaac gccaacgccc gtgcgctggc
     4921 gcagcagacc actgaaacgg tcggtctggt cgttggtgat gtttccgatc cgtttttcgg
     4981 tgcaatggtg aaagcggtcg aacaggtggc ttatcacacc ggtaattttt tattgattgg
     5041 caatggttac cacaacgaac aaaaagagcg tcaggccatt gagcaactga tccgccatcg
     5101 ctgtgctgcg ttggtcgtcc atgccaaaat gatcccggat gccgatttag cctcattaat
     5161 gaaacaaatg cccggtatgg tgctgatcaa ccgtatcctg cctggcttcg aaaaccgttg
     5221 tattgctctg gacgatcgtt acggtgcctg gctggctacg cgtcatttaa ttcaacaagg
     5281 tcatacccgc attggttatc tgtgctctaa ccactctatt tctgacgccg aagatcgtct
     5341 gcaagggtat tacgatgccc ttgctgaaag tggtattgcg gccaatgacc ggctggtgac
     5401 atttggtgaa ccagacgaaa gcggcggcga acaggcaatg accgagcttt tgggacgagg
     5461 aagaaatttc actgcggtag cctgttataa cgattcaatg gcggcgggtg cgatgggcgt
     5521 tctcaatgat aatggtattg atgtaccggg tgagatttcg ttaattggct ttgatgatgt
     5581 gctggtgtca cgctatgtgc gtccgcgcct gaccaccgtg cgttacccaa tcgtgacaat
     5641 ggcgacccag gctgccgaac tggctttggc gctggcggat aatcgccctc tcccggaaat
     5701 cactaatgtc tttagtccga cgctggtacg tcgtcattca gtgtcaactc cgtcgctgga
     5761 ggcaagtcat catgcaacca gcgactaacc gcagttaaag caattccagc gccagtaatt
     5821 cttcgatggt ctggcgacgg cgaatcaacc gcgcctgacc attatcaaac agaacttctg
     5881 gtaacagcgg acggctgttg tagttggatg acattgatgc gccatatgcc cctgtatcat
     5941 gcagtaccag ataatcaccc gccttcactt ccggcaaggc gcgggtttca acatttcccc
     6001 cttcctgctg ggtaaagaca tcgcccgatt cacataatgg cccggcgacg acggtttcca
     6061 ccgttggcgc gtgttccaga gaacgaccat cagctgccag ggcactgata tggtggtaac
     6121 taccgtacat tgccgggcgc atcagatcgt tgaacccggc atcaaccagc acaaagtggc
     6181 ggctacccat ttgtttgacg ctccgcacct gagtaattaa cacgccagac tgcgctacca
     6241 gaaagcgacc cggttcaatt tccagtttca cagggtggcc caaatggcga gcgatttgct
     6301 cacgcgcggc attccacaga ccataataat gttcggtatc aaccgcctct tcaccctgtt
     6361 gataaggaat agaaagccca ccgcccgcag aaatagcctg taaatcctga ccgaattcga
     6421 tgacctgacg taccatagca ccacacacct gttccagatg ggcataatca acgccagaac
     6481 caatgtgcat gtgaatgccg accagctgca gatgatgacg ttgtatcacg tccagtgcgg
     6541 cgggcagatc ggtgtaccag ataccgtgct tgctgttttc gccaccggta ttggtttttt
     6601 ggctatgtcc gtgaccaaac cccgggttaa cgcgcagcca tacccgatgc cctggcgaaa
     6661 cctggcccag ttggtcgagc atatcaacag aacccgcatt caccggaatt tgcaattcac
     6721 tgacgcgttc aagcgtcgcc tgatcgataa catctgccgt aaaaacaata tcatcggggt
     6781 gcgtttgcgg attgtaaccc gccgccaacg cacgctctat ttcgcctaac gagacggaat
     6841 ccactttcac gccctgctca cgcattaatc gcaaaatatg aatattggaa caggctttct
     6901 gtgcaaagcg caccacatca aactgtttca gcgctgcaat ctgccgacga ataatttgcg
     6961 catcgtagac ccacaccggg cagccaaatt cagcgggcaa acgcagcaga ttttcggcgg
     7021 tgagatcggt atcggtgctg aacagtgaat gtggcataac aaactccaga tgagtgcttt
     7081 tttatgatta cgccacatca taaaaagaat aaaaaatatc gatttatgtc gagtctatgc
     7141 aaaaatgata tagattaccg gattgcgaga gagcgctaat ggccgccgtt aacttacgtc
     7201 atattgaaat ttttcatgcg gtaatgaccg ccggaagcct gactgaggca gcacacctgc
     7261 tacacacctc acagccaacc gtcagccgcg aactggcgcg ctttgagaag gtgatcgggc
     7321 taaaattgtt tgagcgcgta cgtggacgat tacatcctac cgtgcaagga ctgcgtctgt
     7381 ttgaagaagt gcaacgatcc tggtacggac tggatcgcat tgtcagcgcc gcagaaagtc
     7441 tgcgcgagtt tcgccaggga gaactgtcta ttgcctgcct gccggtcttt tcgcaatctt
     7501 ttttaccgca gctcctgcaa ccctttctgg cacgttatcc cgatgtcagc ttaaatatcg
     7561 tgccccagga atcaccgcta cttgaagagt ggctctcggc ccagcgtcat gatttaggac
     7621 tcactgagac actccatacg cctgcgggaa cagaacgtac cgaattactc tctttagatg
     7681 aagtgtgtgt gttacctccg ggtcatccgc tggcggtaaa aaaggtatta acgccggatg
     7741 attttcaggg tgagaactac atcagccttt cccgtactga cagctatcgc cagttgctgg
     7801 atcagctatt tactgaacat caggttaaac gacgcatgat cgtagaaacc cacagcgccg
     7861 cgtcagtctg cgcaatggta cgggcggggg taggtgtttc ggtggttaac ccactcaccg
     7921 cactggatta tgcggcaagc ggtttagtgg tacggcggtt cagtattgcg gttccgttca
     7981 ccgtcagcct gatccgcccc ctgcaccgcc cgtcatcagc gctggttcag gcgtttagtg
     8041 agcatttaca agcggggtta ccgaaactgg tcacttctct tgacgcgatt ttgtcgtcag
     8101 ctacgacagc ataaaagcga cagcatcctc ggcatggatc gccgcagtat caaacacagg
     8161 caaaacactg cgctcttctg gaaccaataa accaatttct gtgcagccaa aaatgacacc
     8221 ctgcgccccc tgttctgcaa ggcgagcaat cacttgcgca taataagcgc gtgacgcttc
     8281 ggtaaattgc cccagacaca gttcttcaaa aataatctga ttaatcttcg cccgttcatc
     8341 cgcttccgga ataagacaat tgatggaaaa ttattccgtc agccgcccgc gataaaaatc
     8401 ctgttccatg gtgtaacgcg tacccagcag cgccacacga gtcattccgg ccccggtaat
     8461 tgcacgtccg gtggcatccg ccactttatg catcgtattg gtacataaca caataccttc
     8521 tgcgcccgcc cgctgtaagc caagcgccgc ctcagccaga atgtccccgg ttttatccca
     8581 ttccccgcga cgctggcact cttctatttc atgaaaatcg acgctatgta gcagcacttg
     8641 cgcagagtga agcccaccaa gccgctgttt aatgccttca tttatcagac gatagtaagg
     8701 aatggtggat tcccagctca ttcctcccag caaaccaatt gttttcattc ctctctctcc
     8761 tgatgtgtgt taccgcaaca aggctgaata aggaacgaga caaacgcctc aacggccagg
     8821 tgcccaatct ctattaacga aaaaagggcc ggatgtacag cacatccggc ccgtgaaatc
     8881 agacgccgat atttctcaac ttctcgcctg ccatcagttt gcgttcgata tgttccagcg
     8941 tgacattttt ggtttccgga atgagccaga aagtgatgcc cacaaacgca atgttcagcg
     9001 cagtgtagag ccagaacgta ccggcagcac caatgctgtc gagcagcgtc aggaaggtcg
     9061 cgccgataat catattcgac acccagttcg tggtggtcga acaggtaata ccgaaatcgc
     9121 ggcatttcag cggctgaatt tcagagcaca ggatccacac cactggcgcg gcgctcatcg
     9181 cataaccggc aatacacatc atcgtcatgc caacagagag ccaggacaag ccactggaag
     9241 ccgtaccgtt atcaaactgc atcaggcaat agcccagcac cagagtgcct aacgccatca
     9301 cgctgaaacc aattttcaga gccggtttac gccctgcttt atctaccgta aacaccgcaa
     9361 taaaggtggc gaacataaag gtcagcccta cgaccagagt cgcaatcatc tgttgttctg
     9421 tggtcgtaaa gcccgccatt ttgaagatac gcggcgcgta gtacatgatg atgttcatac
     9481 cggtaaactg ctgcatcgcc tgcaacaaca taccgagaaa cacagcacga cggacgttac
     9541 ggttgatctt aaacagtgcc caaccgccct gttttaactt caggctttca cgaatttcgt
     9601 tgagttcttc tcgcgctttt tccgacgtat cgcgcagcat acgcaacact tcttccgcct
     9661 caatatgacg ccccttttcc gccagccagc gcgggctatt tggcaggaag accaccagaa
     9721 taatcagcag gactgctggt aaagcaagaa cccccaacat tgcgcgccag ttaccgctat
     9781 aactgaacgc tgtatcggat aaaaacgcca gcacgatgcc gagtgtgacc atcaactggt
     9841 acatactgat catcttaccg cgaacgtttt cacttgccat ttcagaaaga tacagaggag
     9901 cggtgtaaga cgcgatcccg acagcaatgc ccagcaccac ccgagcggcg attaacatct
     9961 ctacgctggt cgcaaaagcg gaccctatag aaccgagtac aaacaggatg gcccccgcca
    10021 tcaggctgta tttacgcccc aggcggaacg acagccaacc attaaacagc gcaccaattg
    10081 ctgcgccgag catcatgcta ctaaccaccc attcctgcaa acgactggtc agcacaaagt
    10141 gatcggtaat gaacggcaac gctccggcga ttacgccgat atcaagacca aataacaatc
    10201 ctgcgaccgc agcagctacc gaaacaaaca tattcatacg ccgcgtatcc cgcaaagaac
    10261 gtggcgttaa agcagattcc gtattgatag taaccatttt ttcctgccag cagagagtaa
    10321 gacatagtga aaaaatacgt gaacaactca cacaggtgtc aggtcggaaa cagcataaat
    10381 atggattaaa ttgctgcgac atgtcgttat gtgatggata ttccaatttt caaattaagt
    10441 tgaattattg agattattat taaccaccta attttacagc agataaaatt cataaagttc
    10501 attaattgat aattgatatg gattatttca taaccatgat atgaattatg atgacctaca
    10561 ggtataaaaa accctgccat gaggcagggt cataaaagta agaggaatga attaacgagc
    10621 cagccaaccg ccatctacgg caatggtata accattcaca taatctgaag cgctggaggc
    10681 aaggaacact atcggcccca tcaggtcact cggcagtccc caacgaccag ctggaatgcg
    10741 gtcgagaatt tccgcgctac gttgttcatc tgcccgtagt tgttgagtat tgttggtcgc
    10801 catgtaaccc ggagctatcg cattaacatt aatgttgtgt ttagcccatt cgttcgccat
    10861 caatcgcgtc acgcccatca cgccgctttt tgatgcggta taagaaggca cacggatccc
    10921 gccctggaag gagagcattg acgtgatatt gataatcttg ccgccattgc gttgcgcgat
    10981 aaagtgtttc gccgctgcct gagacatgaa gaatacgctc ttgatattca ggttcatgac
    11041 atcgtcccag tctttttcgc tgaactcgag agcatcttcg cggcgaatca atccggcgtt
    11101 attcaccagg atatcaatat gaccaaactc agctaccgcg cgatccagca gtgctggaat
    11161 gccatcaatc tttcgcagat cggcggtcag gcttaaaaaa cgacgcccca gcgccgtgac
    11221 ctgcttgatg gtttcagtcg gttcaacgat gttaatgcca acaatgtcac agcccgcttg
    11281 cgccagcccc aacgccatcc cctggcccag tccagtatca caaccagtga cgaccgcaac
    11341 tttaccttcg agagaaaatg cacttaaaat cataacaata ccttattcgt ttatgccgac
    11401 aactagcgca aatctttaac ggccactatg ccgacaacta gcgcaaatct ttaacggcca
    11461 catggtccat atcatcaaag acctggtttt caccaaccat gccccagata aaggtataag
    11521 ctttggttcc gacgccggaa tggatcgacc agctggggga gatcaccgcc tgctcgttat
    11581 gcatcacaat gtgacgcgtt tcttgcggct gccccatcat gtggaaaacg caggcgtcat
    11641 catccatatt gaaatagaaa taaacttcca tccggcgctc gtgagtgtga cacggcatgg
    11701 tgttccacaa gttacctgga gccagctccg tcagccccat actcaattgg caggtttcca
    11761 gcacatccgg gacaaaatat ttgttaattg tgcgacggtt actggtgagg ttatcgccta
    11821 acgtgactgg agatacttcg tccggtgtga cttttttggt gggatacgtc gtatgcgcgg
    11881 gtgcgcaatt gtaataaaac tttgccggag tggcggtatc aatactggca aagacaactt
    11941 cttttgcacc tttaccaaca tacagggcgt cgcggtgacc gatttcatag cattggccat
    12001 cgacagtaat cgtaccggca ccgccaatat tgataacacc taactcgcga cgttcaagga
    12061 aatagcttac gccgagttgt ttaccaactt ccccgccaac ggaaaccgtt ttagttatcg
    12121 gcataatgcc gccaacaata attcggtcaa tgtggctgta aaccatggtg tactcatcgg
    12181 cgacaaatac cttttcaacc aaaaattcat tgcgcagccc ttgggtatcc agcgtttttg
    12241 cgtgcgcact gtggatgctc tgtcttacgt ccacatcaac ctccgataat cagtgaagcg
    12301 tgaaaataga aaacgatagt gaaacactgt ttcgttttct ctataaaggg atgatatgcc
    12361 catgggctag ggttttcaat taaaaataaa acaatgtttc atttttaagt taaggattaa
    12421 aaaaagtgca gtgtcgatca cgaatatgtc atgagaatgg aaaaatgagg gagcccagaa
    12481 aatgtatgtc gacttgccag ccatcattat tcaacagatt aatgacggca gatatttatc
    12541 ggtaaggagg aaatgcagag ctactcgtca cgttcaatgg tcaatgccac gccctggccc
    12601 ccgccgatac aaagcgttgc cagccctttg cgggcattac gtttcaccat ttcatgaacc
    12661 agagaaacca ggattcggca accggaagcg cctatcgggt gaccgagtgc gatcgcgcca
    12721 ccattgacat tgacccgacg ctcatcccac tcaagcatct tgccaaccga aagcgcctgt
    12781 gcagcaaacg cttcattagc ctcgataaga tcgacatcag ccaactgcca gcctacacgc
    12841 tccaggcaac ggcgggtcgc atacaccggc gcaattccca tcaatgccgg atctacacca
    12901 acgctggcaa atgcgcggat gcgggccagc acgggtaaat tcaacgctcg tgctttggct
    12961 tcgctcatca tcattaccgc agctgcgcca tcgtttatgg atgatgcatt ccccgcggtc
    13021 acagaaccga gactatcaaa tgaaggattt aaacgggcta aaccttctgc actggcgtca
    13081 gtgcgtggct gttcatcggt atcaacaacc aacgtctgac cgttactttg gatcattacc
    13141 gggacgatct catctttaaa tcgtccggcg tcaatcgccg ctcgcgcttt ttgttgcgag
    13201 ctaagtgcgt aagcatcctg caactgacgg ctgatgccat attcgcgagc cagattttcg
    13261 gcggtgacac caatatgata atcattgaag gcatcccaca acccatcatg caccagactg
    13321 tcaaccaact ggctattgcc aagctgtgca ccggtgcggc tatcagtcag aacatgtggt
    13381 gcgcggctca tgttttcctg gccaccggcg atgacaatat cagcctcgcc acactgtatc
    13441 gcctgagtag ccagatgcag tgctttaagt ccggaaccgc aaacgtcatt aatcgtgatt
    13501 gcagaaacgc tattaggaag accaccttta atagccgatt gccttgccgg attctgccct
    13561 gcccctgcag tcaacacctg accaagaatt acttcatcca ccgcatatgc aggaacgccg
    13621 gtacgttcta ttaacgcttt cacgaccaga ctaccaagtt ccacggcgga atgacccgct
    13681 aacgcaccac gaaagcagcc gataggtgtc cgtaacgccc cgacaatcac aacgtctttc
    13741 atcacaacct cgcaccaatt aacagtgcaa tagtaaatga ttgttaatta cagttatctt
    13801 aattgtttaa aaaaagtgat ttttatcaca aaggaaatat gcctgagcag tagtcagagt
    13861 cataactggc acgtaaggtt tgcaaccact aacccaccaa tagatgggta gataaggcgt
    13921 taatctccca tacttaacct ggtttatggt aaattgccct ccattttgtt taatttgtag
    13981 atgatacgtt cagataatgt ctaatatttg gtctaaagaa gaaactctgt ggagtttcgc
    14041 gctctacggc acagccgttg gtgcaggcac gctcttcctt cctattcagt taggttcggc
    14101 aggggctgtg gtcctgttta ttactgctct ggtcgcctgg cctttaacat attggccaca
    14161 taaagcctta tgccagttca tcctctcatc aaaaacatca gcatgtgaag ggataacggg
    14221 cgcggtaaca cactactatg gcaagaagat tggtaatctg attaccacgc tgtacttcat
    14281 cgcctttttt gtcgtcgtgt tgatatatgc agtggcaatt accaactcac ttacggaaca
    14341 gctggcaaag catatggtta ttgatcttcg catccgtatg ttggtgagtc tgggtgttgt
    14401 attaattctg aatctcattt ttctgatggg acgtcatgcc actattcggg taatgggatt
    14461 tttggtattc ccattgattg cctatttctt atttctttcc atttaccttg tcggtagttg
    14521 gcaacctgat ctattaacga cccaggtaga gttcaatcag aatacccttc accagatatg
    14581 gatatcgatt cccgtgatgg ttttcgcctt tagccatacg cccattattt ctacgtttgc
    14641 catagacaga cgtgaaaaat atggcgaaca cgctatggat aaatgcaaaa aaattatgaa
    14701 agtcgcttat ctcatcatct gcataagtgt actgttcttt gtctttagct gcctgctttc
    14761 tattccacct tcgtatattg aagcggctaa agaagaaggg gtcaccattt tatcggcgct
    14821 ttctatgctg ccgaacgccc cagcatggtt gtcaatttcc gggattattg tcgcagtagt
    14881 tgcgatgtcg aaatcattcc tgggtacgta ctttggcgtt attgaaggtg ccacggaggt
    14941 cgtcaaaaca acactacagc aggttggtgt aaagaaaagt cgtgcattta accgcgcact
    15001 atcaattatg ttggtatcgc tgattacctt cattgtttgt tgcattaacc cgaacgcgat
    15061 ttcgatgatt tatgcgatca gcggcccgct cattgccatg atacttttca tcatgcctac
    15121 gttgtcaacg tatctgatcc cggcgcttaa accctggcgc tccatcggaa atctgattac
    15181 gctgatcgtg ggtatcctgt gcgtatcggt aatgttcttt agctaatatc cgttgtggat
    15241 ggttcaaaat tgctacaatt ttgaaccatt ccatttttca taatattcat ttgttattat
    15301 tccgccctat cacttctctc gtcatatcct agaattatca gttagttaaa cacacaaaga
    15361 gatctgaaca tcttaaataa gactatttaa gatgcataac ttagattcgc aagatatctt
    15421 ctggtcattc aaaaacaatt tgcgatatag atattctcat accatgagat agttatctgg
    15481 accacgttcg accatgcaag attaaatcag tgaaacataa tcatatttaa taacgagaaa
    15541 tgcattttta aatgtcttaa tcagaattat agcaaataca gattaatcgc aatttttccc
    15601 agcaaaatta caaaatcaag atgtattgac atcatcaatc accaatgaaa ttatgcaatt
    15661 atatacggat agggaggttc ttaacatggg gctttgtagt cgttataaaa gtcttacatg
    15721 caatagttgc tcaatgcatt gccaaataat gccagaagag tcaccgcgtt tgcagtattg
    15781 cgcaaactcg tgtttttgta tgtggcccga agaaagttca tattttaatc gtggtgtggt
    15841 ggaaggtatc ttaacaaaaa accacaatgc gagattaagc ggatatattt ttgtggattt
    15901 ttcagtaagt tttttacgtc tattccttga aaaagactgg attgactatc ttgcaagtac
    15961 tgacatgggg atagttttag tcagcgaccg caatatgcaa tcgttagcta actactggcg
    16021 aaaacacaac accgcaatat ctgctgtcat atataatgat gatggtcttg atgttgccaa
    16081 tgaaaagatc agacaactat ttattggtcg ttatttatca tttaccagag gtaacacatt
    16141 aactcagatg gaattcacca taatggggta tatggtttct ggttataatc catatcagat
    16201 tgctgaagtg ctggatatgg atatccgtag catctacgcg tacaagcaac gaatcgaaaa
    16261 gagaatgggt ggtaaaataa acgaattatt tattcgttca cattcggtcc aacattgata
    16321 ctcaacgacc agccagaatc atactctgtt catacgggca tctattgaaa tgcccgtatt
    16381 ttataaataa gcagtaaccc caagagtaaa caacatagta tttaaaacca tcacacgaaa
    16441 aattcagaag cattacga
//
LOCUS       DAJVRF010000088        16286 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJVRF010000088 DAJVRF010000000
VERSION     DAJVRF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16286
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.088"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..578
                     /locus_tag="OQN13_002615"
     CDS             <1..578
                     /locus_tag="OQN13_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCS1615936.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENSYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            581..1744
                     /locus_tag="OQN13_002616"
     CDS             581..1744
                     /locus_tag="OQN13_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS1615937.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1828..3453
                     /locus_tag="OQN13_002617"
     CDS             1828..3453
                     /locus_tag="OQN13_002617"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCS1615938.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="OQN13_002618"
     CDS             complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="OQN13_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCS1615939.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="OQN13_002619"
     CDS             complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="OQN13_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCS1615940.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4505..5254
                     /gene="yjfP"
                     /locus_tag="OQN13_002620"
     CDS             4505..5254
                     /gene="yjfP"
                     /locus_tag="OQN13_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1615941.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="OQN13_002621"
     CDS             complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="OQN13_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCS1615942.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="OQN13_002622"
     CDS             complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="OQN13_002622"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCS1615943.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7533..8931
                     /gene="ulaA"
                     /locus_tag="OQN13_002623"
                     /pseudo
     CDS             7533..8931
                     /gene="ulaA"
                     /locus_tag="OQN13_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            8947..9252
                     /gene="ulaB"
                     /locus_tag="OQN13_002624"
     CDS             8947..9252
                     /gene="ulaB"
                     /locus_tag="OQN13_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCS1615944.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9262..9726
                     /gene="ulaC"
                     /locus_tag="OQN13_002625"
     CDS             9262..9726
                     /gene="ulaC"
                     /locus_tag="OQN13_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCS1615945.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9740..10390
                     /gene="ulaD"
                     /locus_tag="OQN13_002626"
     CDS             9740..10390
                     /gene="ulaD"
                     /locus_tag="OQN13_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCS1615946.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10400..11254
                     /gene="ulaE"
                     /locus_tag="OQN13_002627"
     CDS             10400..11254
                     /gene="ulaE"
                     /locus_tag="OQN13_002627"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCS1615947.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            11254..11940
                     /gene="ulaF"
                     /locus_tag="OQN13_002628"
     CDS             11254..11940
                     /gene="ulaF"
                     /locus_tag="OQN13_002628"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCS1615948.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATYLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            12037..12588
                     /locus_tag="OQN13_002629"
     CDS             12037..12588
                     /locus_tag="OQN13_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HCS1615949.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(12663..12978)
                     /locus_tag="OQN13_002630"
                     /pseudo
     CDS             complement(12663..12978)
                     /locus_tag="OQN13_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            13305..13700
                     /gene="rpsF"
                     /locus_tag="OQN13_002631"
     CDS             13305..13700
                     /gene="rpsF"
                     /locus_tag="OQN13_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCS1615950.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13707..14021
                     /gene="priB"
                     /locus_tag="OQN13_002632"
     CDS             13707..14021
                     /gene="priB"
                     /locus_tag="OQN13_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCS1615951.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            14026..14253
                     /gene="rpsR"
                     /locus_tag="OQN13_002633"
     CDS             14026..14253
                     /gene="rpsR"
                     /locus_tag="OQN13_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCS1615952.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14295..14744
                     /gene="rplI"
                     /locus_tag="OQN13_002634"
     CDS             14295..14744
                     /gene="rplI"
                     /locus_tag="OQN13_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCS1615953.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14856..15827)
                     /locus_tag="OQN13_002635"
     CDS             complement(14856..15827)
                     /locus_tag="OQN13_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCS1615954.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(15880..>16286)
                     /locus_tag="OQN13_002636"
     CDS             complement(15880..>16286)
                     /locus_tag="OQN13_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCS1615955.1"
                     /translation="SNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDMTPAV
                     LIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPINKIIK
                     PMLPFYAVMVLVLAMVTYIPQISMALPKALGY"
BASE COUNT         3824 a         3978 c         4332 g         4152 t
ORIGIN      
        1 ttggtagacc gaataatcct tttgaaccgc gtcgcaatcg ctacgcacaa aaatacttaa
       61 cgttggcgct aatgggcggt gccgcttttt tcgtattgaa aggttgtggc gatagtagcg
      121 atgtcgataa cgacggcgac ggaacgtttt acgcgacagt gcaggattgt attgatgacg
      181 gtaataatgc cgacatttgc gcgcgtggct ggaacaacgc caaaacggca ttttatgccg
      241 atgttccgaa gaatatgact cagcagaact gtcagtctaa gtacgaaaat tcctactacg
      301 acaatgttga acagagttgg atcccggtcg tttctggatt tttgttaagc cgggttattc
      361 gtaaagatcg cgatgagccg ttcgtctata acagcggcgg atcctcattt gcttcccgcc
      421 ccgtctggcg caacacttcc ggtgattact cctggcgctc cggttctggc aaaaaagagt
      481 cttactcttc aggcggcttt accaccaaaa aagcgtcaac cgtttcgcgc ggcggctatg
      541 gtcgttcttc cagcgcccgt gggcattggg gaggctaatc atgctgagac acaacgttcc
      601 tgtgcggcgg gatctggacc aaattgccgc tgacaacggt ttcgactttc atatcatcga
      661 caatgaaatc tattgggacg agagtcgggc ttaccgtttt actctgcgcc agattgaaga
      721 gcagatcgaa aaaccaactg cggaactgca tcagatgtgc cttgaggtgg tggatcgcgc
      781 ggttaaagac gaagagatcc tgacgcaact ggcgatcccg ccgttgtact gggatgtgat
      841 cgccgaaagc tggcacgcca gcgatccttc gctgtatggc cggatggatt ttgcctggtg
      901 tggcaatgcg ccggtgaagc tgctggagta caacgccgat acgccgactt cgttgtatga
      961 gtcggcttat ttccagtggt tgtggctgga ggatgcccgg cgcagcggca ttattccgcg
     1021 tgatgccgat cagtacaatg ccattcagga acgcctgatt tcacgcttta gcgagcttta
     1081 tagccgggag ccgttttatt tctgctgctg tcaggacacc gatgaagaca ggactaccgt
     1141 gctgtacttg caggactgcg cccagcaggc agggcaggag tcgcggttta tctacattga
     1201 agatctcggt ttgggcgtcg gcggtgtact gaccgatctt gatgataatg tcatccagcg
     1261 tgcatttaag ctgtatccgc tggagtggat gatgcgtgac gataacggtc cgctgctgtg
     1321 caagcgtcgc gagcaatggg tggagccgtt atggaaaagt atcttgagta ataaagggct
     1381 aatgccgctg ctttggcgct tcttccctgg tcatcctaac cttcttgcat cctggtttga
     1441 gggtgaaaaa tcgcagattg ccgctggcga aagctatgtg cgtaaaccga tctactcgcg
     1501 cgaaggcgga aacgtcacca ttttcgacgg tcagaataac gtcgttgacc acgctgatgg
     1561 tgattacgcc gatgaaccga tgatctacca ggcgtttcaa cctctgccgc gatttggcga
     1621 tagctacaca ctcatcgata gctggattgt cgatgatgaa gcgtgcggaa tggggatccg
     1681 tgaagataac acactgatca ccaaagacac ctcacgtttc gttccgcatt acattgctgg
     1741 ataagaatgt tttagcaatc tctttctgtc atgaatccat ggcagtggcc atactaatgg
     1801 tgactgccat tgatggaggg agacacagtg cactggcaaa ctcacaccgt ttttaatcaa
     1861 cctataccat taaataacag caacttatac ctgtctgatg gcgcgctttg cgaagcggta
     1921 acgcgtgaag gtgctggctg ggatagcgat tttctagcca gtattggtca gcagttagga
     1981 acggctgaat cccttgaact ggggcggctg gcgaatgtaa acccgcctga actgttgcgc
     2041 tacgatgccc aggggcggcg tctggacgat gttcgtttcc atcccgcatg gcacctgctg
     2101 atgcaggtgt tatgtaccaa tcgggtgcac aatcttgcct gggaagaaga cgctcgctcc
     2161 ggcgcatttg tggcgcgcgc ggcgcgtttt atgttacatg cgcaggttga ggctgggtcg
     2221 ttatgtccaa taacgatgac ctttgccgcc acgccattgt tgttacagat gttacccacg
     2281 ccgtttcagg actggaccac gccgctgctg agcgatcgct acgattctca cttattgcca
     2341 ggtgggcaaa aacgcggttt gttgattggc atgggaatga cggaaaagca gggtggttcc
     2401 gatgtcatga gcaacaccac ccgtgcagag cgtttggaag atggctctta tcggctggtg
     2461 gggcataaat ggtttttctc ggttccgcaa agcgatgcgc atctggtgct ggcgcagacc
     2521 acgggcggtc tgtcctgttt ttttgtgccg cgctttttgc ctgacgggca acgcaacgcg
     2581 attcgcctcg aacggctgaa agataagctg ggtaatcgct ctaacgccag ttgtgaagtg
     2641 gagtttcagg atgccattgg ctggttattg gggcaggaag gggaaggaat tcgtctgatc
     2701 ctgaaaatgg gggggatgac gcgttttgat tgcgccctgg gtagccatgc catgatgcgc
     2761 cgtgcgtttt cgctggcgat ttatcatgca catcaacgcc atgtttttgg taatccattg
     2821 atccaacagc cccttatgcg tcatgtctta agtcgcatgg cacttcagct tgaagggcaa
     2881 acggcgttgc tgtttcgtct tgcgcgagcg tgggaccggc gcgccgatgc caaagaagcc
     2941 ctgtgggcgc gtttatttac gcctgcggca aaatttgtga tctgcaaacg aggtatgccg
     3001 tttgtggccg aagcgatgga ggtgctgggc ggcattggtt attgcgagga gagcgagctg
     3061 ccgcggcttt accgggagat gccggtaaac agtatttggg aaggttccgg caatattatg
     3121 tgcctggatg tgttgcgcgt tctcaataag caagcgggcg tatacgactt attgtcggaa
     3181 gcatttgtgg aagtgaaagg gcaggatcgc tattttgatc gcgcggttcg tcgtttacag
     3241 cagcagctgc gtaagccagc tgaagaactg gggcgagaga ttactcatca gctattcctg
     3301 ctgggctgcg gtgcgcaaat gttgaaatat gcttctccgc caatggcgca ggcgtggtgt
     3361 caggtgatgt tagatacgcg cggcggcgta cggttgtcag agcagatcca gaatgattta
     3421 ttgctgcggg cgacgggggg agtgtgtttg taaacgtata cgactgatgc gacgctggtt
     3481 tcgattaact caatgaaata tgtgaaaatt gtaggccgga caaggcgctc gcgccgcatc
     3541 cggcattgtt catttgctgt agatgacact catgcataca gtatcgcctg tacgcgccag
     3601 ttatcaggct gctggtcttc gtacatggta ataatgcggt accatgacgc accttgttca
     3661 tcggccttta acgccacgat acgttccacg tcctgcggat cccctgaaat attgctaact
     3721 gagatctgac ctatttcatt caggcccgtc acgcagtcag cactggcgaa ttctgcagac
     3781 tgtgccgtgg cgctcgcagg ataagtcact aattgcagcg agggtgaggc aagtaattgt
     3841 ttcatcgtca gctccatttt tcgtttcccc acatgttgca gggcattcca tcgcggtaat
     3901 aaacgcccag gcttccagcc ggtaatttat tgtgcacagg taaacgcata gggacgcaaa
     3961 gcaatgtaaa gctgactatg taagttgtct gatttattta cggtacaaaa tggcttgtga
     4021 ataccactgt cctgtcacga tggtttcgtc caccatgacg acaacgtaat aatcagcttt
     4081 tgcagcgaca gcttttgctc tgatttctgc taatgcgtca tccggagaac cccgaaccat
     4141 cgtgcttacg ctacctattc gctgtaaccc ttgcgtctgg tcgcggcgaa tctcttgcgg
     4201 atggtccgtt actggcggtg ctggctgtgg cgtaccttgc agtgcgctac aggcacttaa
     4261 catcaacacc aataataaac tggcaaaccg gtaaataacg ctattacgtt tcctgctaac
     4321 catagtgtag tgccttatta atttaacttt gggggaatgt tcccgaattg ttacctggta
     4381 cgcgattttc gaatgaaaac gtgacgaaag cgtaacccac atctcaacat tatgaactaa
     4441 taccgccagc agaaacttgt gctaaggatt tcttcatgtc ataccagaca aaaaggagag
     4501 acagatgatt gaaatagaat cacgcgagct ggcagatatt cccgttcttc atgcttatcc
     4561 tgtcgggcaa aaagataccc cgttaccgtg cgtaattttt tatcacggct ttacttcatc
     4621 cagcctggtg tatagctatt ttgccgttgc gctggcgcag gctggtttgc gggtgatcat
     4681 gccggatgcg cccgatcacg gtagccgttt tagtggcgac gaagcgcggc ggttaaatca
     4741 attctggcaa atcttgctac aaagtatgca ggaattcact actttacgtg cggcaatagc
     4801 cgaagaaaac tggctgcttg atgaccgtct ggcagtcggt ggcgcgtcga tgggcgcgat
     4861 gacggcactg ggaattaccg ctcgccaccc cacggtgaaa tgtaccgcca gcatgatggg
     4921 atcgggctat tttacatcgc tcgcccgttc actgtttcca ccgctgatac ctgaaacggc
     4981 agcacagcag aatgaattca ataacattat cgcgccgctg gcagagtggg aagcgacaaa
     5041 ccacctggaa caacttgggg acagacctct actgctgtgg catggcctcg acgatgacgt
     5101 tgtgcctgcc gacgaatcac tacgtttgca gcaggcctta agcgagacgg gacgggataa
     5161 actgctaacc tgttcatggc agccaggcgt gcgtcaccgc attacgcctg aggcgttaga
     5221 tgctgccgtg acatttttcc gccagcatct ttaaacacgc agaatgctga ccccttgcgc
     5281 ttccagttgt tgcaggattt ccgggttagc atttttgccg gtgataagca tatcgatttg
     5341 atcggcacgg ctaaaaagca ttcccgcgcg ttcgccaatc ttactgctat caaccagtac
     5401 caccagtttc cctaccacgc tcagcatctt ctgctctgcc attgctgtca gcatatcggt
     5461 tttatacaac ccttctgcgg tcagcccttt tccgctggta aacatccagt gcccggcata
     5521 gagactgttt tcgctgccct gcgggcttaa agtgatggac tgacttttgt tgtactgtcc
     5581 acccataatg atcacgctgt catgttcttg atcgatcagg taatttgcca gcggtagata
     5641 attagtgatg atttgcactg gcttgccaca catttcccgc ccaagcagaa acgcggtgga
     5701 gccgcagttg atgactacgc tttcgcccgg attaaccagc tgcgaggccg ctttagcaat
     5761 acgtactttt tcatcgtgat tctgcgcctg atgcagattc atcggcgtcc agcgcgggcg
     5821 ctgttgggta atagcttctg cgccattgcg cacctttttc agtttgccgc gttcgtcaag
     5881 tttattgata tcgcgtcgcg cagtggcagg cgaaattccc agacgctcaa cgactttctc
     5941 aacggtcaca aagcccaatt gtgcgagcat ttccaggagg atttgatgtc tttgtgcttc
     6001 agtcatgagc tattccgata aaatttgatt tttttagatg atatttgaaa tagctaggaa
     6061 atactacgcc ggatagcgcg aacttctcca cgctatccgg tataaacgac agattacagg
     6121 aatgacttga acggcagatc cggttcaata gtgaagcaat catcgaaacc gcgcggatag
     6181 tggtactcga agttgtcttt atccagcggc caggtaaatt tgccgccaac ctgccagata
     6241 aacggcttga agccatactt cagacgatct tttttcatct cccacagcac gcggatctct
     6301 tgcggatcgg cctggaagtt tgaccagatg tcgtggtgga acgggataac cactttcgca
     6361 ttcagggctt cacccatacg cagcatatcg gcgctggtca ttttgtcggt aataccgcgc
     6421 gggttctcac catacgatcc taacgccaca tcgatctgat gttcgttacc gtgcttcgca
     6481 taatagttag agtagtggga atcgccactg tgatataggg aaccgccagg ggttttgaac
     6541 aggtagttta ctgcgcgatc gtccatgcca tctggcagta cgccagccgc tttttgatcg
     6601 gcaggcaggg tgatcagtgc agtacggtcg aaagcgtcaa gcgcatgaat ttcaatgtct
     6661 ttcactttta caacatcgcc cggtttgacc acgatgcaac gctctttcgg tacgccccag
     6721 ccaatccaca aatcaacgca ggttttcggt ccaatgaatg gtacgtcatc agcacagttc
     6781 tgcatcacgg cagcagcgac gttaacgtcg atatgatcgt tgtgatcgtg agtcgccagt
     6841 accgcgtcga tctggcgaat cgcaaacgga tcaagaacaa acggggtggt gcgtaggttt
     6901 ggctgcagtt ttttcacgcc agccatgcgc tgcatctggt gaccctgttt cattaacggg
     6961 ttaccgtgac tttgtttgcc agtgccgcac cagaaatcaa cgcaaacgtt ggtgccacct
     7021 tctgatttca accagatccc ggtgcagcct agccaccaca tcgcaaatgt gccaggggcg
     7081 acctgctctt gttcaatttc ttcattcagc cagctacccc actccgggaa agtgctcagg
     7141 atccaggatt ctcgggtgat acttttcact ttactcatcg ccatttacct tcatgatagt
     7201 tcaattcgaa tcaatatgtg attggttttg attaatcctg acactatttt ttcaggaagg
     7261 caatgaccat tttttgactt tttgccaggg aagttgttgt tgattttgag tgtggaaaga
     7321 tttaatggaa tgtgtagtta aattaactaa atgaatttaa ataaataatt gtttcgttgt
     7381 gtgaatccca ctctatccgt gtggaattat ttgcgggtcg cgtcacattt aatcataaat
     7441 aatcttgttg tgattacttt tgaaaattag agtgagtgca caacattccg ggtgtgcgga
     7501 atacccggtt acctcttctt caggagatcg ttatggagat cctctacaac atctttaccg
     7561 tgttttttaa ccaggtcatg accaatgccc cgttgttgct gggtattgtg acctgtctgg
     7621 gctacatcct actgcgcaaa agtgtcagcg ttattattaa aggcacgatt aaaaccataa
     7681 ttggtttcat gttgttgcag gcagggtccg gcatcctcac cagcaccttc aaaccggtgg
     7741 tggcgaaaat gtccgaagtc tacggcatta aacggcgcaa tttccgatac ttacgcgtcg
     7801 atgatggcaa ccatcgaccg catgggcgat gcctatagct gggtaggtta cgcggtgttg
     7861 ttagcgctgg cgctgaacat ctgttacgtg ctgctgcgtc gcattaccgg tattcgtacc
     7921 atcatgctga ctggtcatat catgttccag caggcggggc tgattgccgt tacgttgttt
     7981 attttcggct actccatgtg gacaaccatt atctgcacgg cgattctggt ttcgctctac
     8041 tggggcatta cctccaacat gatgtacaag ccgactcagg aagtgacgga cggctgcggt
     8101 ttctccatcg gtcaccagca acagtttgca tcatggactg cctataaagt tgcgccgttc
     8161 ctcggcaaaa aagaggaaag cgttgaagac ctcaaactgc caggctggct gaatattttc
     8221 cacgacaaca tcgtctccac ggcgattgtc atgaccatct tctttggtgc cattctgctc
     8281 tccttcggta tcgacaccgt gcaggcgatg gcaggcaaag tgcactggac ggtctatatc
     8341 ctgcaaaccg gtttttcctt cgcggtggcg atcttcatca tcactcaggg tgtgcgcatg
     8401 tttgtggcgg aactctctga agcgtttaac ggtatctccc agcgcctgat ccctggtgcg
     8461 gttctggcga ttgactgtgc ggctatttat agcttcgcgc cgaacgccgt ggtctggggc
     8521 tttatgtggg gcaccatcgg tcagctgatt gcggttggca tcctggtcgc ctgcggttcc
     8581 tcgagcctga ttatcccagg ctttatcccg atgttcttct ccaacgccac catcggcgtg
     8641 ttcgctaacc acttcggcgg ctggcgtgcg gcgctgaaga tttgtctggt gatggggatg
     8701 atcgaaatct ttggttgcgt ctgggcggtg aaacttaccg gtatgagtgc ctggatgggg
     8761 atggcggact ggtcgattct ggcaccgccg atgatgcagg gcttcttctc catcggtatc
     8821 gcctttatgg ccgtcatcat tgtaattgca ctggcttata tgttcttcgc tggccgcgca
     8881 ctgcgcgcag aagaagatgc agaaaaacaa ctggcagaac agtctgctta ataaggagtt
     8941 ttgattatga ccgtacgtat tctggctgtg tgtggcaacg gacaaggcag ttccatgatc
     9001 atgaggatga aagtggacca gtttttaacc caatcaaaca ttgaccacac ggtaaatagc
     9061 tgcgcggttg gcgaatacaa aagcgagttg agcggcgcgg atatcatcat cgcttctacg
     9121 cacattgcgg gcgaaatcac cgtgaccggc aacaaatacg tggttggcgt gcgcaacatg
     9181 ctctctcctg ccgactttgg cccgaaactg ctggaagtga tcaaagcgca tttcccgcag
     9241 gatgtgaagt aaggacgcgc catgaaatta cgtgattcgc tggcggagaa taaatccatc
     9301 cgcctgcagg ctgaagctga gacatggcag gacgcagtaa aaatcggcgt tgacctgctg
     9361 gtggcggcgg atgttgtcga gccgcgttac taccaggcga ttctggatgc ggttgaacag
     9421 cacggtccgt actttgtgct cgcaccaggc ctggcaatgc cgcacggtcg cccggaagag
     9481 ggcgttaaga aaaccggttt tgcgctggtg acgctgaaaa agccgctgga gttcaaccac
     9541 gaagataacg acccggtgga tatcctcatc accatggcgg cggtcgatgc caacacgcac
     9601 caggaagtgg gcatcatgca gatcgtcaac ctgtttgaag atgaagaaaa tttcgaccgt
     9661 ttacgtgcct gccgtaccga gcaggaagta ctggatctca ttgaccgcac caacgcggca
     9721 gcttaagaag gaattgaaga tgtcattacc gatgttgcaa gtcgcgctgg acaaccagac
     9781 tatggatagc gcctacgaaa ccactcgcct gattgccgaa gaagtcgaca ttatcgaagt
     9841 gggtaccatt ctgtgcgtgg gcgaaggcgt gcgtgcggtt cgcgatctga aagcgctcta
     9901 cccgcacaaa atcgtgctgg cagacgccaa aattgccgat gcgggcaaaa ttctttctcg
     9961 tatgtgcttc gaagccaacg ctgactgggt gaccgtgatt tgttgtgcgg atatcaacac
    10021 cgccaaaggt gcgctggacg tagcaaaaga gtttaacggc gacgtgcaga tcgaactgac
    10081 cggttactgg acctgggaac aggcgcaaca gtggcgcgac gcaggtattc agcaggtggt
    10141 ttatcaccgc agccgtgacg cgcaggccgc tggcgtggcg tggggtgaag ccgatatcac
    10201 tgcgatcaaa cgtctttccg atatgggctt caaagtcacc gtcaccggtg gcctggcgct
    10261 ggaagatctg ccgctgttca aaggcattcc aattcatgtg tttatcgccg gtcgcagcat
    10321 ccgtgatgcc gcttctccgg ttgaagccgc gcgtcagttc aaacgttcca tcgccgaact
    10381 gtggggctaa ggagcggata tgttgtccaa acaaatcccg cttggcatct atgaaaaagc
    10441 gctccccgcc ggggagtgct ggctggaacg cctgcgactg gcaaaaacgt tgggcttcga
    10501 ttttgtcgag atgtcggtag atgaaactga cgagcgcctg tcgcgcctcg actggagccg
    10561 cgagcagcgt ctggcgctgg tgaatgcgat tgttgaaacc ggcgtgcgtg tgccgtccat
    10621 gtgcctttct gcccatcgtc gtttcccgtt gggcagcgaa gatgacgcgg tgcgggcgca
    10681 ggggctggag attatgcgta aagccatcca gttcgcccag gacgttggca ttcgcgtgat
    10741 ccagctggcg ggctatgacg tttactatca ggaagccaat aacgaaacgc gtcgtcgttt
    10801 ccgtgacggc ctgaaagaga gcgttgagat ggcgagccgc gcgcaggtga cgctggcgat
    10861 ggagatcatg gattatccgt tgatgaactc catcagcaag gcgctgggtt acgcgcacta
    10921 tctcaacaac ccgtggttcc agctctaccc ggatatcggc aacctgtcgg cgtgggacaa
    10981 cgatgtgcag atggagttgc aggccggaat cgggcatatc gtcgcggtgc atgtgaaaga
    11041 caccaaaccg ggcgtcttca aaaacgtgcc atttggcgaa ggtgtggtgg atttcgaacg
    11101 ttgcttcgaa acgctcaaac agagtggcta ttgcgggccg tacctgattg agatgtggag
    11161 cgaaacggcg gaagacccgg cggcagaagt ggtgaaagcg cgtgattggg tgaaagcgcg
    11221 catggccaaa gcaggcacgg tggaggcggc ataatgcaaa agctaaaaca gcaggtgttt
    11281 gaagccaaca tggatctgcc gcgctatggg ctggtgacct ttacctgggg caacgtcagc
    11341 gctatcgacc gcgaacgcgg gctggtggtg atcaagccca gcggcgttgc ctacgaaacc
    11401 atgaaagcag acgatatggt ggtggtcgat atgagcggca aggtggtgga aggggagtat
    11461 cgcccgtctt ccgacaccgc gacgtatctc gaactctacc gtcgttaccc gtcgcttggc
    11521 ggcattgtcc atacccactc cactcatgct actgcatggg cgcaggcggg gctggcgatc
    11581 ccggcgttag gcactacgca cgccgactac ttctttggcg acatcccgtg tacgcgcgga
    11641 ttaagcgaag aagaggtgca gggcgagtat gaactgaaca ccggcaaagt gattatcgaa
    11701 acgctgggtg acgccgagcc gctgcatacg ccggggattg tggtgtatca gcacggtccg
    11761 ttcgcctggg ggaaagatgc ccacgacgcg gtgcataacg cggtggtgat ggaagaagtg
    11821 gcgaaaatgg cgtggattgc gcgcagtatt aacccacaac tgaatcacat cgacagcttc
    11881 ctcatgaata aacacttcat gcgtaagcac ggtcctaacg cctattacgg gcagaagtaa
    11941 gcagtgcgcc ccggaattat tacctcctgc gggatgtaat tggtctgatt ccggggccgc
    12001 ttcagttaaa aaatcgtata aacaacaggt gtttttatgg ctatcaatgg attgtttgtc
    12061 agaacggatc cgcaacgtcg ccgctatggc gttgcgttac ttgtgggtgt actttcagga
    12121 attatttcag catttgttaa atggggagcg gaagtcccct tgccaccgcg cacattttcc
    12181 ggtggtcgcg atgaatttaa cccgccgtat atctttctac gggattattt aggtatcgac
    12241 ccaacccaaa ctgtgtatac attctcggaa catattatca acccggtgat ggtgacgcat
    12301 atcatcttct cactggtttt tgcgataggg tattgtattg tcgcggaaat tttcccgaaa
    12361 gtgaaattat ggcaggggat attagcaggg cttattgtga cagtggcagt ccacggtata
    12421 ttttgcccgg cgctgaattt aacgccgccg ctcactcaat tgccttttga cgaatatgcc
    12481 tctgaaatcc tggggcatat tttctggttc tggataatag aaattatgcg ccgcgactta
    12541 cgtaatcgca ttactcatga gccagatccg gaagtcgcaa tccggtgaat aaccgcataa
    12601 aaaagccccc tgcgaagggg gcaaagcaaa ctatggcaat gtttcgttgg ttatacctgg
    12661 tgctagcgat aaatatccgc gctggcgtgc atattgccgt tactccccgg ttcccgcatc
    12721 agaattacgt ggtagtacgt tactccccgg ttcccgcatc agaattacgt ggtagtacgt
    12781 tgcgccctgc gcatctgtct cttcatttaa tgcctgacgc gcttcgcttt cagtggcgaa
    12841 attatgattg atataaatca cgcctaagct ttgcacatca tccatgtttc tggcctggtg
    12901 gttattaatt tcaatggctg cccatgtatt tgcacttagc aaaagcacag ccagaagggc
    12961 taaaactcga ctgaacatag atacctcctc gacggctgac tttgtgtgct ctcctctgtg
    13021 atgatcttct gatttaattt taatcaatga taaagaagtt gatggtgacc atttctgatg
    13081 cagttgttca aaaaaacacc atgatgaagt gtgatgaact ccaaatcagc ggtttagagg
    13141 ttaattgcga aaggggagat ttatttcggc tctgcccttg agtttagcgg ggcatacaag
    13201 tactataacg cgtcattttt cagccgacct ttaacacgtt ccttgcctcc ccgggattcg
    13261 gctgacccag acaggaggct gaataatccg taaggagcaa ttcgatgcgt cattacgaaa
    13321 tcgtttttat ggtccatcct gaccagagcg aacaggttcc gggcatgatc gagcgctaca
    13381 ctgctgccat cactggtgca gaaggcaaga tccaccgtct ggaagactgg ggccgccgtc
    13441 agctggctta cccgatcaac aaactgcaca aagcacacta cgttctgatg aacgttgaag
    13501 ctccgcagga agtgatcgat gagctggaaa ctaccttccg cttcaacgat gccgttatcc
    13561 gcagcatggt tatgcgtacc aagcacgctg ttaccgaagc atctccgatg gttaaagcga
    13621 aagacgagcg ccgtgagcgt cgcgatgatt tcgcaaacga aaccgctgat gatgctgaag
    13681 ctggggattc tgaagagtaa tttctgatga ccaaccgtct ggtgttgtcc ggcaccgtgt
    13741 gcaggactcc ccttcgaaag gtcagtccat caggaattcc tcactgccag ttcgtgcttg
    13801 agcatcgttc tgtgcaggag gaagccggtt ttcaccggca ggcgtggtgc caaatgcccg
    13861 ttattgttag cggacacgaa aaccaggcca ttactcacag tataacggtc ggcagtcgca
    13921 taaccgttca ggggttcatt tcatgccaca aggcaaagaa cggactgagc aaaatggttt
    13981 tgcatgccga gcagattgaa ttgatagatt ctggagacta gccatatggc acgttatttc
    14041 cgtcgtcgca agttctgccg tttcaccgcg gaaggcgttc aagagatcga ctataaagat
    14101 atcgctacgc tgaaaaacta catcaccgaa agcggtaaga ttgtcccaag ccgtatcacc
    14161 ggtacccgtg caaaatacca gcgtcagctg gctcgcgcta tcaaacgcgc tcgctacctg
    14221 tccctgctgc cgtacactga tcgccatcag taatcggtca cggtccatta atacgacttt
    14281 gagaggataa ggtaatgcaa gttattctgc ttgataaagt agcaaacctg ggtagcctgg
    14341 gtgatcaggt aaacgttaaa gcgggctatg ctcgtaactt cctggtaccg cagggtaaag
    14401 ctgttccagc taccaagaaa aacattgaat tcttcgaagc acgtcgcgct gaactggaag
    14461 ctaaactggc tgaagttctg gcagctgcta atgctcgcgc tgagaaaatc aatgcactgg
    14521 aaactgttac catcgcgtct aaagctggcg acgaaggtaa actgttcggt tccatcggta
    14581 ctcgcgacat cgctgacgct gtaactgcag ctggcgttga agtggctaag agcgaagttc
    14641 gtctgccgaa cggcgttctg cgtaccactg gcgagcacga agtgagcttc caggttcaca
    14701 gcgaagtatt cgcgaaagtg atcgtaaacg tagtagctga ataattcgtt attcaacgag
    14761 acgtaaaaac gccgaccagt ggtcggcgtt ttgcttttgt agcgagcact tgcagatatc
    14821 gataagggcc acaataagca gccctacgaa gataattact tagctgcctg aatatcagcc
    14881 agcattgggg caagggctgg gttgtcctta gcgaagtttg cgtacattgg ctgtactttc
    14941 tcgataaacg gagctttatc tacctggtag actttcacgc ccattttatc cagttttgca
    15001 aattcttgtt tctcttcctc gctccacaag tctttcatgt agctcattga ttctttagct
    15061 gcttttaata taactgcctg attttccggg ctaagtttat caaacgcagc attgctcatc
    15121 agaaccacat cgggaaccat agtgtgttcg tcaaggctga aggattttgc cacttcaccg
    15181 tgacgcatgg tggtcagcgc catcacgctg ttttctgcca tatcgaccac accctgctgt
    15241 aaggctgtat acaactcgcc gtaatccagt ggcgtcgggt taccgcccat gactttgatc
    15301 atttcaactg cgccagggct tggctggaca cggactttca tgcctttcag atcgtctggt
    15361 ttcagtactg gtttattgcc atagaaactg cgggcgcctc catcataaaa agtcagcccg
    15421 aaataacctt tgcttttcgt tgagtcgagg atttttctcc cgatatcgcc ctgcatgacc
    15481 tgataataat gatcacgatc gcggaacaaa taaggcaggc taaataattt ataggaattt
    15541 tcaaatgctt ccatttctgc cgcattggtt tttaccagtg gaatagcgcc agaacgaatc
    15601 agctccattg tttctcgttg agtacccaat gttccatttg gataaatacg aatagtaata
    15661 tcaccgttag agtactcttt gttctttttc gcaaagaact ccatcgcttt atgaacagga
    15721 tgagacttat cctgattatg actcagtttt aatgtcactg cccaggactg gcttgcggat
    15781 aacataagaa cagcaccgat aatgccagcg gatactttta ttttcataga tgttcctttt
    15841 ctaataaata tggcggcagg tgccgccagt gaatgcgcat cagtagccca gtgcttttgg
    15901 caatgccata ctgatttgcg gaatataggt caccattgcc agcaccaata ccatcacggc
    15961 atagaatggc agcataggtt tgataatttt attaatcggt aatttagata cactacaacc
    16021 gacaaataac acactgccta caggcggagt acaaataccg atacacatgt tcagtaccat
    16081 gacaatacca aagtgaatcg ggtccatgcc cagttcggtg acaacaggta aaaatatcgg
    16141 cgtaaaaatc agtaccgccg gtgtcatatc cataaaagtg ccgataatca acaggataat
    16201 attgataata agcaggatta ttattgggtt gtcagaaata gtgttaagca aatcgagaat
    16261 caggaaggga acgtcagcgt ttgaca
//
LOCUS       DAJVRF010000089        16076 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJVRF010000089 DAJVRF010000000
VERSION     DAJVRF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16076
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.089"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(1..567)
                     /locus_tag="OQN13_002637"
                     /pseudo
     CDS             complement(1..567)
                     /locus_tag="OQN13_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(801..2221)
                     /gene="espX1"
                     /locus_tag="OQN13_002638"
                     /pseudo
     CDS             complement(801..2221)
                     /gene="espX1"
                     /locus_tag="OQN13_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
     gene            complement(2706..2969)
                     /gene="rpsT"
                     /locus_tag="OQN13_002639"
     CDS             complement(2706..2969)
                     /gene="rpsT"
                     /locus_tag="OQN13_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCS1615956.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            3072..3290
                     /locus_tag="OQN13_002640"
     CDS             3072..3290
                     /locus_tag="OQN13_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCS1615957.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            3298..4239
                     /gene="ribF"
                     /locus_tag="OQN13_002641"
     CDS             3298..4239
                     /gene="ribF"
                     /locus_tag="OQN13_002641"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCS1615958.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQKEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKASMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            4282..7098
                     /gene="ileS"
                     /locus_tag="OQN13_002642"
     CDS             4282..7098
                     /gene="ileS"
                     /locus_tag="OQN13_002642"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCS1615959.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            7098..7592
                     /gene="lspA"
                     /locus_tag="OQN13_002643"
     CDS             7098..7592
                     /gene="lspA"
                     /locus_tag="OQN13_002643"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCS1615960.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            7717..8166
                     /gene="fkpB"
                     /locus_tag="OQN13_002644"
     CDS             7717..8166
                     /gene="fkpB"
                     /locus_tag="OQN13_002644"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1615961.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            8168..9118
                     /gene="ispH"
                     /locus_tag="OQN13_002645"
                     /gene_synonym="lytB"
     CDS             8168..9118
                     /gene="ispH"
                     /locus_tag="OQN13_002645"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCS1615962.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            9184..10098
                     /gene="rihC"
                     /locus_tag="OQN13_002646"
     CDS             9184..10098
                     /gene="rihC"
                     /locus_tag="OQN13_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCS1615963.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            10265..11086
                     /gene="dapB"
                     /locus_tag="OQN13_002647"
     CDS             10265..11086
                     /gene="dapB"
                     /locus_tag="OQN13_002647"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCS1615964.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
     gene            11542..12690
                     /gene="carA"
                     /locus_tag="OQN13_002648"
     CDS             11542..12690
                     /gene="carA"
                     /locus_tag="OQN13_002648"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCS1615965.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            12708..15929
                     /gene="carB"
                     /locus_tag="OQN13_002649"
     CDS             12708..15929
                     /gene="carB"
                     /locus_tag="OQN13_002649"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCS1615966.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
BASE COUNT         3924 a         3783 c         4253 g         4116 t
ORIGIN      
        1 atcatccccc cagtagaaag gaatggagat aaacagatta aaacgctcct cttctttatt
       61 attttcatca taagattggc tggcagagaa agtataacta atgtgttgcc agttattgga
      121 gtaactgaat tggtaatctt tagcatttcc acttcgcccc cagtaattcc gccaaagcgc
      181 acttaatgat acatttccca gattattgga taaaggttgc ataatattgg cagaaaggct
      241 attttttctg ccaatatcat aaaaatcatc ataatcactc tgatgatagt gtttatcatt
      301 ttcgtaaaga tggtcgctga atgtcctgta atcctgcgaa gcataacgcc aggcggccac
      361 gctaaatcga gtggctgttt gtactagata tttattatag gcgacctgat agctcgtacc
      421 ttcgtgtctg gtatcattat ttaacttact actcgatcgg gcagcgtaaa aagagatggc
      481 acccagcggt gtattccaac catttcccag agttatagca ttatagttat cggataaaat
      541 cgtacccgat tatgtgatca ggttgttatt atttagatta atgtttaaat tttttaattt
      601 cttgatgtga agttgatgaa agtttgaaaa tttaatattc ttaattgttc ccttctttat
      661 aagaagagca aggtgccatc attagagtga cagagtagga tgtttcagag catgcttcag
      721 attgtttttt atttctctgc agtgtattaa taatcatcac agataatttc attctaaaat
      781 tacctctttc tttgagtgtt ttaatcctga tgaactatat tttttataag gagcatgcac
      841 tcgttgatgc tctgaaggta ttttctcatt tttgatgatg ttttgtcgta atcgtgcata
      901 aacttattaa gttctttata cacatcatta tttagcttta cattatcgcg aactaaagtt
      961 tgtaccaatg gtatcaaaat ttcgaaacgg gacggtttgt catcaatact tgccagggtt
     1021 ttgaatactc ctgtgtatct gtaacctaag tgcttattga caagttgagt ggtccatcgg
     1081 ccaggcataa tatcttcaca gcgcaaattt tttatcatgg tattagtgaa cgatgttcca
     1141 aatatgttgc tttttttaat gaagaggttg tttatttctc catttgaaaa gatggtgttt
     1201 gataagtcgc tttcaaaaca tgatatattg gttaatacac aacaaaaata atacccatat
     1261 gaaaggttgc tcttttctat tgttatattt gatagcaaca ttttatgtat attaatatta
     1321 agagcagtca aatactggat cttttgatcg ttaagttcat acatctccag tgatttatta
     1381 tagggtaata aaagtataaa ttcctttata ttatctccgc agtaagaaaa gacgacatta
     1441 aatttattag tggccatgtt ttcagttgag cagtaagctg atttttctat taaccgactg
     1501 ggcacgcttt taaataaaga taattttcca catagttttg aatatgccga attgctagtt
     1561 ttaaagttct gccacattat tccctggtta tcatctatcg atgaggaggg gattttattg
     1621 cattgctcat tgtatagctc agggaaaata ttttttttaa taagcttttt gatttcctgg
     1681 ttaagatgac tttttttctt gtctattgca ctgtattttg cgttttgtga cgtttcctga
     1741 tgatcataaa atgactggag atttctgcag gcctcataaa atatttcagg agtatatagc
     1801 tgtgggtata tttcttggtt gaagtttgcc tttaaaaaat taacgaattt ttcatggcat
     1861 tcttggtata tcttaatctc ttcagaaaga cattcgcttg ttctgtcatc aaaaacatcc
     1921 aatctaagta atgtgatacc tttgtgttta aaaaataaaa ttgaactgta tgttctatga
     1981 gtttacatct ataaaaatat gcatctctca gatgagtata ttgaaaaaaa tgatgaagga
     2041 ctggagatgg tttaggcaag ttatggcttc taaaaaagtc atcaaacaaa attaagaact
     2101 ctgttttata ctcgcaaaat acttttacta actcattttg tcttgtttta tttattatta
     2161 catcatctac tatatttgat ttataaagac aatgtggcga agttgagata ccatttgtca
     2221 ttatcagtcc ttgaagttga tgaattttta ttaatttaat ttatgagtga atgtgactac
     2281 tatcaaaaat agctcattaa ttttatgaag tagaatataa ataggtattt aacctgtgtt
     2341 gattgttgtt aattatttac aagatctaat aaggagatat ttatgaatgg ttaaagtgta
     2401 taggaattta attcaaaaat agagtggtct taagtttaat acctcaatgt gtatcacaat
     2461 atccacattc tttgtagggg agtctggaga ttgagtagat attcttgttc agaatgtatc
     2521 agccgatggt tctacgattc ttaagccacg aagagttcag aaagtgtaac ggcatgtctg
     2581 caaaagccag catcttttga ctatctggca accggtagtg tgttctctca cgcatcataa
     2641 aagcagcagg cataaaaaaa acccgcttgc gcgggctttt tcacaaagct tcagcaaatt
     2701 ggcgattaag ccagtttgtt gatctgtgca gtcaggttag ccttatgacg tgcagctttg
     2761 tttttgtgga tcagaccttt agcagcctga cggtccacga tcggttgcat ttcgttaaat
     2821 gctttctgtg cagcagcttt gtcgccagct tcgatagctg cgtatacttt cttgatgaaa
     2881 gtacgcatca tagagcgacg gcttgcgttg tgcttacgag ccttttcaga ctgaatggcg
     2941 cgcttcttag ctgatttgat attagccaag gtccaactcc caaatgtgtt ctatatggac
     3001 aattcaaagg ccgaggaata tgccctttta gccttctttt gtcaatggat ttgtgcaaat
     3061 aagcgccgtt aatgtgccgg cactcgttac gtagtgatgg cgcaggattc taccagcttg
     3121 cggggtgtga atacagcttt tccgcgataa aaattgcagc aggcggtcac tttcttcccg
     3181 tgatttgcgc catggcaatg aaaagccact tctttctgat ttcggtactc aatcgccggt
     3241 taaccttgac cgctgtacaa ggtatactcg gacgattttc actgttttga gccagacatg
     3301 aagctgatac gcggcataca taatctcagc caggccccgc aagaagggtg tgtgctgact
     3361 attggtaatt tcgacggcgt gcatcgcggt catcgcgcgc tgttacaggg cttgcagaaa
     3421 gaagggcgca agcgcaactt accggtgatg gtgatgcttt ttgaacctca accactggaa
     3481 ctgtttgcta ccgataaagc cccggcaaga ctgacccggc tgcgggaaaa actgcgttac
     3541 cttgcagagt gtggcgttga ttacgtgctg tgcgtgcgtt tcgacaggcg tttcgcggcg
     3601 ttaaccgcgc aaaatttcat cagcgatctt ctggtgaagc atttgcgcgt aaaatttctt
     3661 gccgtagggg atgatttccg ctttggcgct ggtcgtgaag gcgatttctt gttattacag
     3721 aaagctagca tggaatacgg cttcgatatc actagtacgc aaactttttg cgaaggtggc
     3781 gtgcgtatta gcagcaccgc tgtgcgtcag gcgctggcgg atgacaatct ggctctggca
     3841 gaaagtttac tggggcaccc gtttgccatc tccgggcgtg tagtccacgg tgatgaatta
     3901 gggcgcacta taggtttccc gacggcgaat gtaccgctac gccgtcaggt ttccccggtg
     3961 aaaggggttt atgcggtaga agtgctgggc ctcggtgaaa agccgttacc cggcgtggca
     4021 aacatcggaa cacgcccaac ggttgccggt attcgccagc agctggaagt gcatttgtta
     4081 gatgttgcaa tggaccttta cggtcgccat atacaagtag tgctgcgtaa aaaaatacgc
     4141 aatgagcagc gatttgcgtc gctggacgaa ctgaaagcgc agattgcgcg tgatgaatta
     4201 accgcccgcg aattttttgg gctaacaaaa ccggcttaag cctgttatgt aatcaaaccg
     4261 aaacacggaa ccgagaatct gatgagtgac tataaatcaa ccctgaattt gccggaaaca
     4321 gggttcccga tgcgtggcga tctcgccaag cgcgaaccgg gaatgctggc gcgttggact
     4381 gatgatgatc tgtacggcat catccgtgcg gctaaaaaag gcaaaaaaac cttcattctg
     4441 catgatggcc ctccttatgc gaatggcagc attcatattg gtcactcggt taacaagatt
     4501 ctgaaagaca ttatcgtgaa gtccaaaggg ctttccggtt atgactcgcc gtatgtgcct
     4561 ggctgggact gccacggtct gccgatcgag ctgaaagtag agcaagaata cggtaagccg
     4621 ggtgagaaat tcaccgccgc cgagttccgc gccaagtgcc gcgaatacgc ggcgacccag
     4681 gttgacggtc aacgcaaaga ctttatccgt ctgggcgtgc tgggcgactg gtcgcacccg
     4741 tacctgacca tggacttcaa aactgaagcc aacatcatcc gcgcgctggg caaaatcatc
     4801 ggcaacggtc acctgcacaa aggcgcgaag ccagttcact ggtgcgttga ctgccgttct
     4861 gcgctggcgg aagcggaagt tgagtattac gacaaaactt ctccgtccat cgacgttgct
     4921 tttcaggcgg tcgatcagga tgcactgaaa gcaaaatttg ccgtaagcaa cgttaacggc
     4981 ccaatctcgc tggtgatctg gaccactacg ccgtggactc tgcctgcgaa ccgcgcaatc
     5041 tctattgcac ctgatttcga ctatgcgctg gtgcagatcg acggtcaggc cgtgattctg
     5101 gcgaaagatc tggttgaaag cgtaatgcag cgtatcggcg tgaccgatta caccattctc
     5161 ggcacggtaa aaggtgcgga gcttgagttg ctgcgcttta cccatccgtt tatgggcttc
     5221 gacgttccgg caatcctcgg cgatcacgtt accctagatg ccggtaccgg tgccgttcac
     5281 accgcgcctg gccacggccc ggacgactat gtgatcggtc agaaatacgg cctggaaacc
     5341 gctaacccgg ttggcccgga cggcacttat ctgccgggca cttatccgac gctggatggc
     5401 gtgaacgtct tcaaagcgaa cgacatcgtc gttgcgctgc tgcaggaaaa aggcgcgctg
     5461 ctgcacgttg agaaaatgca gcacagctat ccgtgctgct ggcgtcacaa aacgccgatc
     5521 atcttccgcg cgacgccgca gtggttcgtc agcatggatc agaaaggtct gcgtgcgcag
     5581 tcactgaaag agatcaaagg cgtgcagtgg atcccggact ggggccaggc gcgtatcgag
     5641 tcgatggttg ctaaccgtcc tgactggtgt atctcccgtc agcgcacctg gggcgtaccg
     5701 atgtcactgt tcgtgcacaa agacacggaa gagctgcatc cgcgtactct cgaactaatg
     5761 gaagaagtgg caaaacgcgt tgaagttgac ggcatccagg cgtggtggga tcttgatgcg
     5821 aaagagatcc tcggcgatga agctgatcag tacgtgaaag tgccggacac attggatgta
     5881 tggtttgact ccggatctac ccactcttct gttgttgacg tgcgtccgga atttgccggt
     5941 cacgcagcgg acatgtatct ggaaggttct gaccagcacc gtggttggtt catgtcttcc
     6001 ctaatgatct ccaccgcgat gaaaggcaaa gcgccgtatc gtcaggtact gacccacggc
     6061 tttaccgtgg atggtcaggg tcgcaagatg tctaaatcca tcggcaatac cgtttcgccg
     6121 caggatgtga tgaacaaact gggcgcggat attctgcgtc tgtgggtggc atcaaccgac
     6181 tacaccggtg aaatggccgt ttctgacgag atcctgaaac gtgctgccga tagctatcgt
     6241 cgtatccgta acaccgcgcg cttcctgctg gcaaacctga acggttttga tccagcaaaa
     6301 gatatggtga aaccggaaga gatggtggta ctggatcgct gggccgtagg ttgtgcgaaa
     6361 gcggcacagg aagacatcct caaggcgtac gaagcatacg atttccacga agtggtacag
     6421 cgtctgatgc gcttctgctc cgttgagatg ggttccttct acctcgacat catcaaagac
     6481 cgtcagtaca ccgccaaagc ggacagtgtg gcgcgtcgta gctgccagac tgcgctgtat
     6541 cacatcgcag aagcgctggt gcgctggatg gcaccaatcc tctccttcac cgctgatgaa
     6601 gtgtggggct acctgccggg cgaacgtgaa aaatacgtct tcaccggtga gtggtacgaa
     6661 ggcctgtttg gcctggcaga cagtgaagcg atgaacgatg cgttctggga cgagctgttg
     6721 aaagtgcgtg gcgaagtgaa caaagtcatt gagcaagcgc gtgccgacaa gaaagtgggt
     6781 ggctcgctgg aagcggcagt aaccttgtat gcagaaccgg aactgtcggc gaaactgacc
     6841 gcgctgggcg atgaattacg atttgtcctg ttgacctccg gcgctaccgt tgcagactat
     6901 aacgacgcac ctgctgatgc tcagcagagc gaagtactca aagggctgaa agtcgcgttg
     6961 agtaaagccg aaggtgagaa gtgcccacgc tgctggcact acacccagga tgtcggcaag
     7021 gtggcggaac acgcagaaat ctgcggccgc tgtgtcagca acgtcgccgg tgacggtgaa
     7081 aaacgtaagt ttgcctgatg agtcaatcga tctgttcaac agggctacgc tggctgtggc
     7141 tggtggtagt cgtgctgatt atcgatctgg gcagcaaata cctgatcctc cagaactttg
     7201 ctctggggga tacggtcccg ctgttcccgt cgcttaatct gcattatgcg cgtaactatg
     7261 gcgcggcgtt tagtttcctt gccgatagcg gcggctggca gcgttggttc tttgccggta
     7321 ttgcgattgg tattagcgtg atcctggcag tgatgatgta tcgctcgaag gccacgcaga
     7381 agctaaacaa tatcgcttac gcgctgatta ttggcggcgc gctgggcaac ctgttcgacc
     7441 gcctgtggca cggcttcgtt gtcgatatga tcgacttcta cgtcggcgac tggcacttcg
     7501 ccaccttcaa ccttgccgat actgccatct gtgtcggtgc ggcactgatt gtgctggaag
     7561 gttttttgcc ttctagagcg aaaaaacaat aataaaccct gccggatgcg atgctgacgc
     7621 atcttatccg gcctacagat tgctgcgaaa tcgtaggccg gataaggcgt ttacgccgca
     7681 tccggcaaaa atcctcaaat ataagagcaa acctgcatgt ctgaatctgt acagagcaat
     7741 agcgccgtcc tggtgcactt cacgctaaaa ctcgacgatg gcaccaccgc cgagtctacc
     7801 cgcaacaacg gtaaaccggc gctgttccgc ctgggtgatg cttctctttc tgaagggctg
     7861 gagcaacacc tgttggggct gaaagtgggc gataaaacca ccttctcgtt ggagccagat
     7921 gcggcgtttg gcgtgccgtc accggacctg attcagtact tttcccgccg tgaatttatg
     7981 gatgcaggcg agccagaaat tggcgcaatc atgcttttta ccgcaatgga tggcagtgag
     8041 atgcctggcg tgatccgcga aattaacggc gactccatta ccgttgattt caaccatccg
     8101 ctggccgggc agaccgttca ttttgatatt gaagtgctgg aaatcgatcc ggcattggag
     8161 gcgtaacatg cagatcctgt tggccaaccc gcgtggtttt tgtgccgggg tagaccgcgc
     8221 tatcagcatt gttgaaaacg cgctggccat ttacggcgca ccgatatatg tccgtcacga
     8281 agtggtacat aaccgctatg tggtcgatag cttgcgtgag cgtggggcta tctttattga
     8341 gcagattagc gaagtaccgg acggcgcgat cctgattttc tccgcacacg gtgtttctca
     8401 ggcggtacgt aacgaagcaa aaagccgcga tttgacggtg tttgatgcca cctgtccgct
     8461 ggtgaccaaa gtgcatatgg aagtcgcccg cgccagccgt cgtggcgaag agtcgattct
     8521 catcggccac gccgggcatc cggaagtgga aggtacaatg ggccagtaca gtaacccgga
     8581 agggggaatg tatctggtcg aatcgccgga cgatgtgtgg aaactgacgg tcaaaaacga
     8641 agagaagctc tcctttatga cccagaccac gctgtcggtg gatgacacgt ctgatgtgat
     8701 cgacgcgctg cgtaaacgct tcccgaaaat tgtcggtccg cgcaaagatg acatctgcta
     8761 cgccacgact aaccgtcagg aagcggtacg cgccctggca gaacaggcgg aagttgtgtt
     8821 ggtggtcggt tcgaaaaact cctccaactc caaccgtctg gcggagctgg cccagcgtat
     8881 gggcaaacgc gcgtttttga ttgacgatgc gaaagatatc caggaagagt gggtgaaaga
     8941 ggttaaatgc gtcggcgtga ctgcgggcgc atcggctccg gatattctgg tgcaaaatgt
     9001 ggtggcacgt ttgcagcagc tgggcggtgg tgaagccatt ccgctggaag gccgtgaaga
     9061 aaacattgtt ttcgaagtgc cgaaagagct gcgtgtcgat attcgtgaag tcgattaagt
     9121 cattagcagc ctaagttatg cgaaaatgcc ggtcttgtta ccggcatttt ttatggagaa
     9181 aacatgcgtt tacctatctt cctcgatact gaccccggca ttgacgatgc cgtcgccatt
     9241 gccgccgcga tttttgcccc cgaactcgac ctgcaactga tgaccaccgt cgcgggtaat
     9301 gtctcggttg agaaaaccac ccgcaacgcc ctgcaactgc tgcatttctg gaatgcggag
     9361 attccgctcg cccagggagc cgctgtgccg ctggtacgcg caccgcgtga tgcagcatct
     9421 gtgcacggcg aatcgggaat ggctggctac gactttgttg agcacaaccg aaagccaatc
     9481 gggataccag cgtttctggc gattcgtgat gccctgatgc gtgcaccaga gcctgttacc
     9541 ctggtggcca ttggcccgtt aaccaatatt gcgctgttac tttcacaatg cccggaatgt
     9601 aaaccgtata ttcgtcgtct ggtgatcatg ggcggttctt ctggtcgcgg caactgtaca
     9661 ccaaatgccg agtttaatat cgctgccgat ccagaagcgg ctgcctgtgt cttccgtagc
     9721 ggaatcgaaa tcgtcatgtg cggtctggat gtcaccaatc aggcaatatt aacccctgac
     9781 tatctcgcta ctttgccgga gttgaatcgc accggaaaaa tgcttcatgc actgtttagc
     9841 cactaccgta gcggcagtat gcaaagcggt ctgcgaatgc acgatctctg cgccatcgcc
     9901 tggctggtgc gcccggactt attcaccctc aagccgtgtt ttgtcgcggt agaaactcaa
     9961 ggcgaattta cctcaggcac gacggtggtt gatatcgacg gttgcctggg caaaccagcc
    10021 aatgtaaagg tggcattgga tctggatgtg aaaggcttcc agcagtgggt ggctgaggtg
    10081 ctggctctgg cgtcgtaacc tgtcacatgt tatcggcatg cagtcattca tcgactcatg
    10141 cctttcactg atatccctcc ctgtttatca ttaatttcta attatcagcg tttttggctg
    10201 gcggcgtagc gatgcgctgg ttactctgaa aacggtctat gcaaattaac aaaagagaat
    10261 agctatgcat gatgcaaaca tccgcgttgc catcgcggga gccggggggc gtatgggccg
    10321 ccagttgatt caggcggcgc tggcattaga aggcgtgcag ctgggcgcgg cgctggagcg
    10381 cgaaggatct tctttactgg gcagcgacgc cggtgagctg gctggtgcag ggaaaacagg
    10441 cgttaccgtg caaagcagcc tcgatgcggt aaaagatgat tttgatgtgt ttatcgattt
    10501 tacccgtccg gaaggtacgc tgaaccatct cgctttttgt cgccagcatg gcaaagggat
    10561 ggtgatcggc actacggggt ttgacgaagc cggtaaacag gcgattcgtg acgccgccgc
    10621 cgatattgcg attgtctttg ccgccaattt tagcgttggc gttaacgtca tgcttaagct
    10681 gctggagaaa gcagccaaag tgatgggtga ctacaccgat atcgaaatta ttgaagcaca
    10741 tcatagacat aaagttgatg cgccgtcagg caccgcactg gcaatgggag aggcgatcgc
    10801 ccacgccctt gataaagatc tgaaagattg cgcggtctac agtcgtgaag gccacaccgg
    10861 tgaacgtgtg cctggcacca ttggttttgc caccgtgcgt gcaggtgaca tcgttggtga
    10921 acataccgcg atgtttgccg atattggcga gcgtctggag atcacccata aggcgtccag
    10981 ccgtatgaca tttgctaacg gcgcggtaag atcggctttg tggttgagtg gtaaggaaaa
    11041 tggtcttttt gatatgcgag atgtacttaa tctcaataat ttgtaaccac aaaatatttg
    11101 ttatggtgca aaaataacac atttaattta ttgattataa agggctttaa tttttggccc
    11161 ttttattttt agtgttatgt ttttaaatat tctataagtg ccaaaaatta catgttttgt
    11221 cttctatttt tgttgtttta atgtaaattt tgaccatttg gtccactttt tcctgctcgt
    11281 ttttatttca tgcaatcttc ttgctgcgca agcgttttcc agaacaggtt atatgatctt
    11341 tttgtcgctt aatgcctgta aaatatgcat gagccacaaa ataatataaa aaataccgcc
    11401 attaagttga cttttagcgc ccatatctcc agaatgccgc cgtttgccag aaattcgtcg
    11461 gtaagcagat ttgcattgat ttacgtcatc attgtgaatt aatatgcaaa taaagtgagt
    11521 gaatattctc tggagggtgt tttgattaag tcagcgctat tggttctgga agacggaacc
    11581 cagtttcacg gtcgggccat aggggcaaca ggttcggcgg ttggggaagt cgttttcaat
    11641 acttcaatga ccggttatca agaaatcctc actgatcctt cctattctcg tcaaatcgtt
    11701 actcttactt atccccatat tggcaatgtc ggcactaatg acgccgatga agaatcttct
    11761 caggtacatg cacaaggtct ggtgattcgc gatctgccgc tgattgccag caacttccgt
    11821 aataccgaag acctctcttc ttacctgaag cgccataaca tcgtggcgat tgccgatatc
    11881 gatacccgta agctgacgcg tttactgcgc gagaaaggcg cacagaatgg ctgcattatc
    11941 gcaggcgata acccggatgc ggcgttggcg ttagaaaaag cccgcgcgtt cccaggtctg
    12001 aatggcatgg atctggcaaa agaagtgacc accgcagaag cctatagctg gacacaaggg
    12061 agctggacgt tgaccggcgg cctgccagaa gcgaaaaaag aagacgagct gccgttccac
    12121 gtcgtggctt atgattttgg tgccaagcgc aacatcctgc ggatgctggt ggatagaggc
    12181 tgtcgcctga ccatcgttcc ggcgcaaact tctgcggaag atgtgctgaa aatgaatcca
    12241 gacggcatct tcctctccaa cggtcctggc gacccggccc cgtgcgatta cgccattacc
    12301 gccatccaga aattcctcga aaccgatatt ccggtattcg gcatctgcct cggtcatcag
    12361 ctgctggcgc tggcgagcgg tgcgaagact gtcaaaatga aatttggtca ccacggcggc
    12421 aaccatccgg ttaaagatgt ggagaaaaac gtggtgatga tcaccgccca gaaccacggt
    12481 tttgcggtgg atgaagcaac attacctgca aacctgcgtg tcacgcataa atccctgttc
    12541 gacggtacgt tacagggcat tcatcgcact gataaaccgg cgttcagctt ccagggtcac
    12601 ccggaagcca gccctgggcc acacgacgca gcgccgttgt tcgaccactt tatcgagtta
    12661 attgagcagt accgtaaaac cgctaagtaa tcaggagtaa aagagccatg ccaaaacgta
    12721 cagatataaa aagtatcctg attctgggtg cgggcccgat tgttatcggt caggcgtgtg
    12781 agtttgacta ctctggcgcg caagcatgta aagccctgcg cgaagagggt taccgcgtca
    12841 ttctggtgaa ctccaacccg gcgaccatca tgaccgaccc ggaaatggcc gatgcgacct
    12901 acatcgagcc gattcactgg gaagtagtac gcaagattat tgaaaaagag cgcccggacg
    12961 cggtgctgcc aacgatgggc ggtcagacgg cgctgaactg cgcgctggag ctggagcgtc
    13021 agggcgtgtt ggaagagttc ggcgtgacta tgattggtgc gaccgccgat gcgattgata
    13081 aagcagaaga ccgccgtcgt ttcgacgtag cgatgaagaa aattggtctg gaaaccgcgc
    13141 gttccggtat cgcacacacg atggaagaag cgctggcggt tgccgctgac gtgggcttcc
    13201 cgtgcattat tcgcccatcc tttaccatgg gcggtagcgg cggcggtatc gcttataacc
    13261 gcgaagagtt tgaagaaatt tgcgcccgcg gtctggatct ctccccaacc aaagagctgc
    13321 tgattgatga gtcgctgatc ggctggaaag agtacgagat ggaagtggtg cgtgataaaa
    13381 acgacaactg catcatcgtc tgctctatcg aaaactttga tgcgatgggc atccataccg
    13441 gtgactccat cactgtcgcg ccagcccaaa cgctgaccga caaagaatat caaatcatgc
    13501 gtaacgcctc gatggcggtg ctgcgtgaaa tcggcgttga aaccggtggt tccaatgtcc
    13561 agtttgcggt gaacccgaaa aacggtcgcc tgattgttat cgaaatgaac ccacgcgtgt
    13621 cccgttcttc ggcgctggcg tcgaaagcga ccggtttccc gattgctaaa gtggcggcga
    13681 aactggcggt gggttacacc ctcgacgaac tgatgaacga catcactggc ggacgtactc
    13741 cggcctcctt cgagccgtcc atcgactatg tggttactaa aattcctcgc ttcaacttcg
    13801 aaaaattcgc tggtgctaac gaccgtctga ccactcagat gaaatcggtt ggcgaagtga
    13861 tggcgattgg tcgcacgcag caggaatccc tgcaaaaagc gctgcgcggc ctggaagtcg
    13921 gtgcgactgg attcgacccg aaagtgagcc tcgatgaccc ggaagcgtta accaaaatcc
    13981 gtcgcgaact gaaagatgct ggcgcagagc gtatctggta catcgccgat gccttccgtg
    14041 cgggcctgtc tgtggacggc gtgttcaacc tgaccaatat tgaccgctgg ttcctggtac
    14101 agattgaaga actggtgcgt ctggaagaga aagtagcgga agtgggcatc actggcctga
    14161 acgctgaatt cctgcgccag ctgaaacgca aaggctttgc cgatgcgcgt ctggcaaaac
    14221 tggcgggcgt gcgcgaagcg gaaatccgca agctgcgcga ccagtatgac ctgcacccgg
    14281 tttataagcg cgtggatacc tgtgcggcag agttcgccac cgacaccgct tacatgtact
    14341 ccacttatga agaagagtgc gaagcgaatc cgtctaccga ccgtgaaaaa atcatggttc
    14401 tcggcggcgg cccgaaccgt atcggtcagg gtatcgaatt cgactactgc tgtgtacacg
    14461 cctcgctggc gctgcgcgaa gacggttacg aaaccattat ggttaactgt aacccggaaa
    14521 ccgtctccac cgactacgac acttccgatc gcctctactt cgagccggta actctggaag
    14581 atgtgctgga aatcgtgcgt attgagaagc cgaaaggcgt tatcgttcag tacggcggtc
    14641 agaccccgct gaaactggcg cgcgcgctgg aagctgctgg cgtaccggtt atcggcacca
    14701 gcccggatgc tatcgaccgt gcagaagacc gtgaacgctt ccagcatgcg gttgagcgtc
    14761 tgaaactgaa acaaccggcg aacgccaccg ttaccgctat tgaaatggcg gtagagaagg
    14821 cgaaagagat tggctaccca ctggtggtac gtccgtctta cgttctcggc ggtcgggcga
    14881 tggaaatcgt ctatgacgaa gctgacctgc gtcgctactt ccagacggcg gtcagcgtgt
    14941 ctaacgatgc gccagtgttg ctggatcact tccttgatga cgcggtagaa gttgacgtgg
    15001 atgccatctg cgacggcgaa atggtgctga ttggcggcat catggagcac atcgagcagg
    15061 cgggcgtgca ctccggtgac tccgcatgtt ctctgccagc ctacacctta agtcaggaaa
    15121 ttcaggatgt gatgcgccag caggtgcaga aactggcctt cgaattgcag gtgcgcggtc
    15181 tgatgaacgt gcagtttgcg gtgaaaaaca acgaagtcta cctgattgaa gttaacccgc
    15241 gtgcggcgcg taccgttccg ttcgtctcca aagccaccgg cgtaccgctg gcaaaagtgg
    15301 cggcgcgtgt gatggctggc aaatcgctgg ctgagcaggg cgtaaccaaa gaagttatcc
    15361 cgccgtacta ctcggtgaaa gaagtggtgc tgccgttcaa taaattcccg ggtgttgacc
    15421 cgctgttagg gccagaaatg cgctccaccg gggaagtgat gggcgtgggc cgcaccttcg
    15481 ctgaagcgtt tgccaaagcg cagctgggca gcaactccac catgaagaaa cacggtcgtg
    15541 cgttgctttc cgtgcgcgaa ggcgataaag aacgcgtggt ggacctggcg gcaaaactgc
    15601 tgaaacaggg cttcgagctg gatgcgaccc acggcacggc gattgtgctg ggcgaagcgg
    15661 gcattaatcc gcgtctggtg aacaaggtgc atgaaggccg tccgcacatt caggaccgca
    15721 tcaagaatgg cgaatatacc tacatcatca acaccacttc aggccgtcgc gcgattgaag
    15781 actcccgcgt gatccgtcgc agtgcgctgc aatataaagt gcattatgac accaccctga
    15841 acggtggttt cgctaccgcg atggcgctga atgccgacgc gaccgaaaaa gtaatttcgg
    15901 tgcaggaaat gcacgcgcag atcaaataat agcgtgtcat ggcggatatt tttcatccgc
    15961 taatttgatc gaataagtaa tacggttctc tgatgaggac cgtttttttt gcccattaat
    16021 taaatctttt ggggaatcga tatttttgat gacataagca ggatttagct cacact
//
LOCUS       DAJVRF010000090        15747 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJVRF010000090 DAJVRF010000000
VERSION     DAJVRF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15747
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.090"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            1..1455
                     /locus_tag="OQN13_002650"
                     /pseudo
     CDS             1..1455
                     /locus_tag="OQN13_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(1543..2514)
                     /gene="lpxM"
                     /locus_tag="OQN13_002651"
                     /gene_synonym="msbB"
     CDS             complement(1543..2514)
                     /gene="lpxM"
                     /locus_tag="OQN13_002651"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCS1615967.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(2634..3956)
                     /gene="mepM"
                     /locus_tag="OQN13_002652"
     CDS             complement(2634..3956)
                     /gene="mepM"
                     /locus_tag="OQN13_002652"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCS1615968.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(3972..4904)
                     /gene="znuA"
                     /locus_tag="OQN13_002653"
     CDS             complement(3972..4904)
                     /gene="znuA"
                     /locus_tag="OQN13_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCS1615969.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4983..5738
                     /gene="znuC"
                     /locus_tag="OQN13_002654"
     CDS             4983..5738
                     /gene="znuC"
                     /locus_tag="OQN13_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCS1615970.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            5735..6520
                     /gene="znuB"
                     /locus_tag="OQN13_002655"
     CDS             5735..6520
                     /gene="znuB"
                     /locus_tag="OQN13_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCS1615971.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(6667..7677)
                     /gene="ruvB"
                     /locus_tag="OQN13_002656"
     CDS             complement(6667..7677)
                     /gene="ruvB"
                     /locus_tag="OQN13_002656"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCS1615972.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(7686..8297)
                     /gene="ruvA"
                     /locus_tag="OQN13_002657"
     CDS             complement(7686..8297)
                     /gene="ruvA"
                     /locus_tag="OQN13_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCS1615973.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            complement(8436..8501)
                     /gene="yobI"
                     /locus_tag="OQN13_002658"
     CDS             complement(8436..8501)
                     /gene="yobI"
                     /locus_tag="OQN13_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCS1615974.1"
                     /translation="MYIFITHLFTEYVILKYLLPI"
     gene            8574..9176
                     /locus_tag="OQN13_002659"
     CDS             8574..9176
                     /locus_tag="OQN13_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCS1615975.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(9178..9699)
                     /gene="ruvC"
                     /locus_tag="OQN13_002660"
     CDS             complement(9178..9699)
                     /gene="ruvC"
                     /locus_tag="OQN13_002660"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCS1615976.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(9734..10474)
                     /locus_tag="OQN13_002661"
     CDS             complement(9734..10474)
                     /locus_tag="OQN13_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1615977.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(10503..10955)
                     /gene="nudB"
                     /locus_tag="OQN13_002662"
     CDS             complement(10503..10955)
                     /gene="nudB"
                     /locus_tag="OQN13_002662"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCS1615978.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(11073..12845)
                     /gene="aspS"
                     /locus_tag="OQN13_002663"
     CDS             complement(11073..12845)
                     /gene="aspS"
                     /locus_tag="OQN13_002663"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCS1615979.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            13155..13721
                     /locus_tag="OQN13_002664"
     CDS             13155..13721
                     /locus_tag="OQN13_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1615980.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            14076..14324
                     /locus_tag="OQN13_002665"
     CDS             14076..14324
                     /locus_tag="OQN13_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCS1615981.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            14925..>15747
                     /locus_tag="OQN13_002666"
     CDS             14925..>15747
                     /locus_tag="OQN13_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1615982.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPEIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
BASE COUNT         3997 a         3995 c         3613 g         4142 t
ORIGIN      
        1 gaatttttta taactattac taataatatg acatcaattc ttcgcagtga gtttgatgaa
       61 atatggaaag cgatgctgac cttgcaattc catgacatcc ttcccggctc atgtatttca
      121 agagtttatc atgaaactga aaaggaatat cttaaactag aagcaaaaac cgagaaaatt
      181 atttctgatg cgcagtctac tttattaagt aaaattgaca cttcttctta taaagatccg
      241 cacattttgt ttaataccac ctgttttgct cgtaacgaat ggatcaatat aaacaacaac
      301 tggctcaaag cacgagtaaa ctcttatggt tatgctgtga ttgttccaaa gaataagatc
      361 gtaaatggtc tgaaggcaga atctcaaagt attgaaaata attacattaa acttcttttt
      421 tcagaaagcg gtgatttaat ttcgctatat gataaaagat atggtaaaga atacattact
      481 gaaaatatgc acagtgagat ccgtgcatac cacgaagatg caggtttttt cgcggcctgg
      541 gattttgctt caaattatcg tgatggggaa tcatatgttt tactcgctga aaaaatgaca
      601 actgtgatca gcgggcctaa aactacgatg acgctgattt atcactacaa ttcctcttat
      661 cttcgtttag cctttaccct gacacaggat agtccacgcg ttgatgtcca aacgtttatt
      721 gattggcacg aacctaatgt ttctctcaaa gtaaaattcc cggtgtcagt acaaacatct
      781 cttgcacaat gtcaaattca gttcggtgta attgacagac caacgcatag cgatgattcg
      841 ttcgcttttg ccaaagatga aataccagca catcactggg tcgacctttc tgatcgagaa
      901 actggcatag ctttaatagc aaaaaacaaa tatggctatc gtgtcaaaga ccagacgctt
      961 gaattaactt tgctgcgttc gcagcataaa cctggggaag ttgttaagac agacaattat
     1021 gataatttcc tgattaataa ttttgcagat attggtaaac aaaaattcat attctcctta
     1081 tttcctcatc atggagatta ccgtgtgggt ggtgttatta atcaagccta tataatgaat
     1141 catcccttac aggtcgtccc tgtcaaaccg cacccgggtg aattaccccc ttcactatca
     1201 ttttttgcta tcgatactaa cagcgttatc atcgaaacaa taaaattagc tgaagacggc
     1261 gacggtataa tcgtaagatt atatgagagt catggattag aaatttcagc catgctttcg
     1321 tgagtatgta attatcacta cgttcaatat gtcgatcttc aagaaaataa gttagatgac
     1381 agtttttatt gcaatcaata ttgcaatctt gagttcaaac cttttgaaat tatcacgcta
     1441 cgattaactc aatagcgtac taacctaaaa actcaaaata gtgggaacac tcattgagat
     1501 tttttatcga gtacagcctc tcaataattt gagaggcttt ttttatttga taggataaag
     1561 atctttacgc ttatacggtt ggatttcgcc cggtttgcga gttttcagca attttaatat
     1621 ccaggtgtat tgttctggtc gcggaccaac aaaaatctcg acttcttcat tcatccgccg
     1681 cgcaatcgta tgatcatccg cctctaacag atcatccatc ggtgggcgca cctgaatcgt
     1741 cagacggtgc gtcttgccat cataaatcgg aaacagcgga acaacgcgcg cacggcacac
     1801 tttcatcaaa cgaccaatcg cgggcaacgt cgctttatag gtggcaaaga aatccacaaa
     1861 ttcgctgtgc tctgggccat gatcctgatc gggtaaataa tatccccagt acccctgacg
     1921 taccgactgg atgaatggtt taataccgtc atttctcgca tgcagacgac cgccaaagcg
     1981 acgacgcacc gtgttccaga cataatcaaa aaccgggttg ccctgattat ggaacatcgc
     2041 tgccattttc tgcccttgcg aggccatcag catggcagga atatcgacgg cccaaccgtg
     2101 cggcaccaga aagataactt tctcgttatt acgcctcatc tcttcgatga tctccagccc
     2161 ttgccagtca acgcgcggct gaattttctc cggtccgcgt attgccaact cagccatcat
     2221 tgccatcgct tgcggcgcgg tggcaaacat ctcatcaaca atcgcttcgc gttcagcttc
     2281 actacgttct ggaaagcaga gcgacagatt gattaacgca cgacggcgtg agctttttcc
     2341 cagtcgtccg gcaaaacgtc ccagccgtgc cagaatggga tcacggaact ttggcggcgt
     2401 taaagcgata cccgccatcg ctgctacacc cagccatgct ccccagtagc gcgggtggcg
     2461 aaaggattta tcaaactcag gaatgtattc gctattattt tttttcgttt ccatgctttt
     2521 ccagtttcgg ataaggcaaa aatcaatctg gtgatagtat agcggcgcaa cttgccccgc
     2581 accaaataaa aaaagccgat actgactgcg taccggctgc gaatggatgt taattaatca
     2641 aaccgtagct gcggcacaat ctctttggcc tgcgccagga attcgcgacg atcggagccg
     2701 gtcagccctt cggtacgcgg cagttttgcc gtcagcgggt ttacggcctg ctggtttatc
     2761 catacttcat agtgcagatg cggcccggtt gaacgtccgg tattaccgga aagcgcgata
     2821 cggtcgccac gtttcacctt ctgtcccggt ttcaccagaa tcttgcgcaa gtgcatataa
     2881 cgcgtggtgt agctgcgacc atgacgaata gccacataat aacctgctgc gccactgcgt
     2941 ttggcaacca ccacttcacc gtcacccact gaaagcactg gcgtaccctg cggcatggcg
     3001 aaatcaacac ctctgtgtgc tgcaacgcga ccggtcaccg gattagtacg acgcgggtta
     3061 aagttagacg agatacggaa ctgtttcgcc gtcgggaatc gcaagaatcc tttcgccaga
     3121 ccagtaccgt tacgatcgta gaatttgcca tcttcagcgc ggatagcgta ataatcttta
     3181 ccttctgaac gcaaacgtac gcccagcagc tggctttgct cacgtttacc atcaagcatt
     3241 tctcgagaca ttaacaccgc aaattcatcg ccttttttca gtttgcggaa atccatttgc
     3301 cactgcatgg ctttaatcac tgcgctcact tcggcgctgg ttaaaccggc gtttctggcg
     3361 ctggcaacaa agctcccccc gacggtacct ttcagcagat tgttaaccca ctctccttgc
     3421 tgcatttcgc tggtcatttt aaaaccgtta gcggcagtac ggtcataggt tcgggtttca
     3481 cgacgagaca cttcccaggt gaggcgctgc agttcgccgt ccgcggttaa tgtccaggag
     3541 agttgttgac cgattttcag gttacgcaat tctttgtcgg ccgcagccag ttgggtgata
     3601 tcacccatat caataccata ctgattgaga atgctgctta gcgtatcgcc agtggaaaca
     3661 acatattcat gcacgcccgc ttcaccggcg attttgtcat ccagttcgtc ctggggaatg
     3721 gcttcatctt cttgtgcagc ttgatcaatc ggctcactgg cttcaggtaa gagcgaacga
     3781 atttcgttct gttccagctc aatggttttg acaattggcg tggcatcgcg gtgataaaca
     3841 tagggccgcc agacagcgac ggccagagta agaacggtga gcgaccccaa cataacgcgg
     3901 tgtggtcgcg gtaaattatt aaacgccagg gcgacagagc gggctatctg ttgcacgtaa
     3961 tcacttcctc attaatctcc tttcaggcag ctcgcatact ggttggctaa ttgactcagg
     4021 aattctgaat agcttgtttt acccagtttg atattcgtcc ccaggggatc caacgttccc
     4081 atacgaacgg atgtccctcg tgcgacgctc tcaacgaccg ctggcctgaa ctgtggctca
     4141 gcaaaaacgc aggttgcttt ttgctcaacc aactgtgttc ttatttcatg taaacgctgc
     4201 gcgccaggtt gaatctcagg gttaacggta aaatgaccaa gcggtgtcag tccgaactgt
     4261 ttttcgaaat agccgtaagc atcgtgaaaa acgaaataac ctttcccctt gagcggcgcg
     4321 agctcgttac caacctgcgt ttcggttgag gctaattgtg cctcaaaatc cttcaggttg
     4381 gcgtcaagtt tggctcgact ttgcggcata agttccacta attttccatg gattgcaacc
     4441 gctgtagccc gcgctatctc tggggaaagc caaagatgca tgttgaaatc gccgtgatgg
     4501 tgatcttcgt cacttttttc cgcgtggtcg tgatcatcat catcgccgtg aatacttttc
     4561 atcagtagcg gtttcacatc ttcaagctgc gcaatcgtta cctgcttcgc tcctggtaat
     4621 ttgcttaccg gtttttgcat aaacgcttcc atctccgggc caacccaaac gactaagtcc
     4681 gcgttctgta agcgttttac atccgatggg cgcagtgaat aatcatgttc tgaagcgccg
     4741 tcaggaagta aaacctctgt ttctgttacc ccatcagcaa tggcagaagc gatgaaccca
     4801 acgggtttaa gcgaagcgac aacggcggca tctgcggcct gtgttgcacc tccccagaga
     4861 gcggcggata atgctgcgaa aagaagcgtt tttttatgta acataatgcg accaatcatc
     4921 gtaatgaata tgagaagtgt gatattataa catttcatga ctactgcaag actaaaatta
     4981 acatgacaag tctggtttcc ttggaaaatg tctcggtttc ttttggccaa cgccgcgtcc
     5041 tctctgatgt gtcgctggaa cttaaacctg gaaaaatttt gactttactt gggccaaatg
     5101 gcgcaggtaa gtcgacactg gtacgggtag tgctcgggct ggtaacaccc gatgaagggg
     5161 ttatcaagcg caacggaaaa ctgcgcatcg gctatgtacc gcagaagctg tatctcgaca
     5221 ccacgttgcc actgaccgta aaccgttttt tacgcttacg ccctggtaca cataaagaag
     5281 atattttgcc tgcactgaaa cgtgtccagg ccgggcacct gattaacgca ccgatgcaaa
     5341 agctctctgg tggcgaaacg cagcgtgtac tgttagcgcg agcattgtta aatcgcccgc
     5401 aattattagt gctggatgaa cccactcaag gcgtggatgt aaatggccag gtggcgttat
     5461 atgaccttat tgaccaactg cgtcgtgaac tggattgtgg cgttttaatg gtttctcacg
     5521 atctgcatct ggtgatggca aaaaccgatg aagtgctgtg cctgaatcac cacatttgtt
     5581 gttccggcac accggaagtt gtttccctgc atccggagtt tatttcaatg tttggtcctc
     5641 gtggtgctga acaactgggt atctatcgcc atcatcataa ccatcgtcac gatttacagg
     5701 gacgaattgt tttgcgtcgg ggaaatgatc gctcatgatt gaattattat ttcccggttg
     5761 gttagccggg atcatgctcg cctgtgccgc gggtccgctg ggttcgtttg tagtctggcg
     5821 tcgtatgtct tatttcggtg atacgctggc tcatgcctca ttacttggtg tcgcgtttgg
     5881 tttgttgctg gacgtgaatc cattctatgc ggtgattgcc gttacgctgc tgctggcggg
     5941 cggtctggta tggctggaga agcgtccaca gctggcgatc gacacgttat tagggattat
     6001 ggcgcacagt gccctgtcgc tgggcctggt ggtcgttagt ctgatgtcta atattcgtgt
     6061 tgatttgatg gcttacctgt tcggtgattt gctggcagtg acgccagaag atctcatctc
     6121 tattgcgatt ggcgtggtca tcgtggtggc tattttgttc tggcaatggc gcaatttgct
     6181 gtcgatgacg attagcccgg atctggcgtt tgttgatggt gtgaaattac agcgcgtgaa
     6241 attgttgttg atgctggtga cggcattgac gattggtgta gcgatgaaat ttgtcggtgc
     6301 gttgattatt acttcgttgc tgattattcc tgcagctact gcgcgtcgct ttgcccgtac
     6361 gccggaacag atggctggtg tcgctgtttt ggtggggatg gtggcagtaa ctggcggttt
     6421 aaccttttcc gcgttttacg atacgccggc gggtccgtcg gtagtgctat gtgctgcact
     6481 gttatttatt atcagtatga tgaaaaagca ggccagctaa tctgtcgctg aacacatttg
     6541 tcggatgcgg cgcgagcgcc ttatccgacc tacggttcgc tatctctggt aggccggata
     6601 agacgcgaat agcgtcgcat caggcacact gccaatgtcg gatgcggtgc gagcgaccaa
     6661 tccgacttac ggcatttctg gcggcgttat gccaaagtga ttccacgccc gcgtcgtcgc
     6721 catacgccca cgcggtgtac gctgcaaaaa gccttgctga atcagataag gctccagcac
     6781 atcctcaatg gtttcacgtt cttcgccaat ggctgccgcc aggttatcca gacctaccgg
     6841 cccaccaaag aacttatcga ttaccgccag caacaatttg cggtccatat aatcgaaacc
     6901 ttcagcatcg acattcaaca tatccagcgc ctgagcagcg atatctgccg agatggtgcc
     6961 atcgtgcttc acttcggcga aatcacgcac tcgacgcagc agacggttgg caatacgtgg
     7021 cgtaccgcga gcgcgacgag caacttccag cgcgccgtca tcactcatct caagccccat
     7081 aaagcgtgcg ctgcgactga cgatatattg cagatccggc acctgataaa actccagacg
     7141 ttgcacaata ccaaaacggt cgcgcaacgg tgatgtcagc gaacctgcgc gcgtggttgc
     7201 accaatcagg gtaaacggcg gcaaatcaat tttaatggag cgtgccgccg gaccttcacc
     7261 aatcatgata tccagttggt agtcttccat tgccgggtac agcacttctt caacaactgg
     7321 cgatagacgg tggatctcat caataaacag cacgtcatgc ggttcaaggt tagtgagcat
     7381 cgctgccaga tcgcccgcct tttccagcac cggaccagaa gtcgtgcgta aattaacgcc
     7441 catttcattg gcgacaatgt tggcaagcgt agttttaccc aaccccggag gaccaaaaat
     7501 caacagatga tcgagggcat cgccgcgcag tttcgctgct ttgatgaaaa tctccatctg
     7561 tgaacgaacc tgcggctgac caacatactc ttccagtaat ttagggcgaa tggcgcgatc
     7621 tgccccatct tccggcaaag tggtaccggc agaaatcaga cggtctgctt caatcatcct
     7681 ttacctcata acgcggcgcg tagggcttcg cgaattaatg tttcactgct ggcgtcagtg
     7741 cgagcgattt tgctcaccat gcggcttgct tcttgtggtt tatagcccag cgccaccagc
     7801 gcggcaaccg cttcctgttc agcatcgtcg gttgccgggc tggcaggcga cgtgagcacc
     7861 agatcggcgg ctggcgtaaa gagatcgccg tgcaaacctt taaatcggtc tttcatttcg
     7921 acaattaaac gctcggcggt ttttttacca atgcccggca gtttcaccag tgctcccact
     7981 tcttcacgtt caacggcatt aacgaactgc tgcgctgaca ttccggagag gatcgccagc
     8041 gccaacttcg ggccgacgcc gttggttttg attaactctt taaacaacgt gcgctcttgt
     8101 ttattgttaa aaccgtacag cagttgcgcg tcttcacgca ccacaaagtg ggtgaaaacg
     8161 atcgcttcct gacccgcttc agggagttca taaaaacagg tcatcggcat atgcacttca
     8221 tagcctacgc cgcccacttc aattaacacc agcgggggtt gtttttcaat gatgatgcct
     8281 ctgagtctgc ctatcacatg acgctcctgc gtaatgaatc aaagataatg ctgtatgata
     8341 aaaaaatgct ggatagatat ccagcgaagg atgaagaaaa cttgcgaggt gtctcgatga
     8401 actgaaaaat ggcacagtat aatttattct acaggttata ttggaagcaa atattttaat
     8461 attacatatt cagtgaataa atgcgtaata aaaatataca ttgcgcctcc tgaaaaaaaa
     8521 taaatttttt atgctattac gtatattcgt atctatttca atggaatgac aacgtgaata
     8581 ttaattaccc tgctgaatat gaaattggag atatcgtctt tacatgtata ggtgctgcct
     8641 tatttggtca aatatcagct gcatcaaatt gctggagtaa tcacgtcggg atcattatcg
     8701 gtcataacgg tgaagacttc ctggttgcag aaagccgggt tcccctttca accatcacca
     8761 cgctatctcg ttttattaaa cgttcgtcta atcaacgcta tgctataaag cgattagacg
     8821 ctgggctgac agaacaacaa aaacaacgaa ttgttgaaca ggttccctcc cggctacgca
     8881 aactttacca caccggtttt aaatacgaat cttcgcgcca gttctgttca aaatttgttt
     8941 ttgatattta taaagaagcg ctatgcattc cggtgggtga aatagaaacg tttggagaac
     9001 tgttaaatag caatccgaat gcaaaactca ctttctggaa attctggttc ttaggttcta
     9061 ttccgtggga gcgtaaaacc gtcacgccag ccagtttgtg gcatcatccg ggtctggtgt
     9121 tgattcacgc ggagggagtt gaaacgcctc agcctgaact gaccgaggcg gtataactta
     9181 acgcagtcgc cctctcgcca ggttcagccg cgattcgctc atctgcattg cattctggct
     9241 gacgtgacaa tgggtaatcg cgatagctaa cgcatcggcg gcatccgcct gtggattagc
     9301 gggcagtttc agcaaggtgc ggaccatatg ctgcacctgg cttttttcgg cactaccaat
     9361 acctaccacc gtttgcttta cctgacgtgc cgcgtattca aataccggca attcctgatt
     9421 caccgccgcc acaatcgcca cgccgcgcgc ctgccccagt ttcagagccg agtcggcatt
     9481 tttggccata aagacttgtt caatggcgaa ataatctggc tggaactggg tgatgatttc
     9541 cgtcacgccc gcatagatga gcttcagacg agacggtaaa tcatccactt tggtgcgtat
     9601 gcatccgcta cccaggtagg acagttgcct gcctacctgg cggatgacac catagccgat
     9661 cacgcgcgaa cccgggtcaa tgccgagaat aatagccatc acgcgtctcc gttttgctgt
     9721 ttagcaggcc ccatcagaga gttgctgcaa cctcatcaga gatttcaccg ttatggtaga
     9781 cttcctgcac gtcgtcgcaa tcttccagca tatcgatcag acgcatcagt ttcggtgcgg
     9841 tttctgcatc catatcagct ttggtggacg ggatcatgga aacttccgcg ctgtctgctt
     9901 tcagacctgc cgcttccaga gcgtcgcgca ctttacccat ttcttcccat gcagtgtaga
     9961 cgtcaatcgc gccgtcatca taggtcacaa cgtcttcagc acctgcttcc agtgctgctt
    10021 ccataatggt gtcttcatcg cctttctcga aggagatcac gccttttttg ctgaacagat
    10081 aggcaacgga accatcagta ccgaggttac cgccacattt gctaaatgca tgacgcactt
    10141 cagcaacggt acggttgcgg ttgtcagaca gacattcaat catgattgcc gtgccgccag
    10201 gaccgtaacc ttcgtagatg atggtttcca tgtttgcatc atcatcaccg cccacaccac
    10261 gtgcaattgc gcggttcagt gtgtcacggg tcatgttgtt agacagtgct ttatccaccg
    10321 ccgcacgcag acgcgggtta gcgtccggat cgccaccgcc cagcttagcc gcggttacca
    10381 gctcacgaat gattttagtg aagattttac cgcgcttagc atcctgcgca gctttacgat
    10441 gtctggtgtt ggcccattta ctatgacctg ccataaaaat atctccagat agccctgcct
    10501 gttcaggcag cgttaattac aaactgttca atcgcctgcc ggttgctcca ggacttagtg
    10561 agcgccgcgg cagcaggcgc atcaagccac ttgtaagcca gatgttcagt gaagactacc
    10621 tggcgttcgt gcgggagcgc aagacagaac catgattccg tattacgcgt cacgcccggc
    10681 gcatagcgat gacgtaaatg tgaaaaaatt tcaaactcta ccgtgcgctg acagtcaatt
    10741 aaggtcagtt gttcagcgac aacatcaatg gtgacctctt cctttacttc gcgcatggca
    10801 gcttgcggcg cggtttcacc ctcttccacg ctgccggtta ccgactgcca gaaatcggga
    10861 tcgtcacgcc gctgcaacat cagcacccgt ttcgtatctt gtgcatagat gaccactaag
    10921 atcgaaacgg gacgcttata cactttatcc ttcacgctgc ctcttcgttg actgccttcg
    10981 ctcatcccat tcacatagtt atctatgctc atgggagttc actcagttgc cgccttgatg
    11041 caacgcgaat gatttcgtgt atttgagtca tatcagttat tctcagcctt cttcacaacc
    11101 tgaatgctca gctcagccag tacagtcggg ttagcaaagc tcggtgcttc agtcatcaga
    11161 cacgctgccg ccgtggtttt cgggaaggcg ataacgtcac ggatattgtc ggtgccagtc
    11221 agcagcatgg tcagacggtc aagaccgaat gccagacctg cgtgcggcgg agtaccgtat
    11281 ttcagggcgt cgagcaggaa gccgaatttc tcgcgctgtt cctcttcgtt gatacccaga
    11341 ataccaaaca ccgtctgctg catatcacca ttatggatac gtactgaacc accgcccact
    11401 tcgtaaccat tgatgaccat atcgtaagcg ttcgccaccg cattttccgg tgcagctttc
    11461 agttctgcag cagtcatgtc tttcggtgag gtgaacggat ggtgcattgc cgtcaggccg
    11521 ccttcaccgt cgtcttcaaa catcgggaag tcgataaccc acagcggtgc ccatttgctt
    11581 tcgtcggtca gaccaaggtc tttacccact ttcaggcgca gtgcgcccat cgcgtcggca
    11641 acaattttct tgttgtcggc accgaagaaa atcatatcgc catcttgcgc ggcagtacga
    11701 tccaggatgg cttcgatgat ttctgcatta aggaacttcg ctaccgggct gttgatacct
    11761 tccagacctt tcgcgcgttc gttaactttg atgtaagcca gacctttcgc gccgtagatt
    11821 ttaacgaagt taccgtattc gtcgatctgc ttacgggtca gcgatgcgcc acccggaaca
    11881 cgtagagcgg caacacggcc tttcggatcg ttcgccggcc ccgcaaatac tgcaaactca
    11941 acagatttca gcagatcggc aacgtcggtc agttccatcg ggttacgcag atccggttta
    12001 tcagaaccat aacggcgttc tgcttccgca aaggtcatta ccgggaaatc gcccagatcc
    12061 acgcccttca cttccagcca caaatgacgc accagcgctt ccatcacttc acgcacttgc
    12121 ggcgcggtca tgaaagaagt ttccacatcg atctgagtaa attcaggctg acgatcagcg
    12181 cgcaggtctt cgtcacggaa gcatttaacg atctgatagt aacggtcaaa accggacatc
    12241 atcagcagct gtttgaacaa ctgtggggat tgcggcagtg cgtagaattt acctttgtgc
    12301 acacgagaag gcaccaagta gtcacgcgcg ccttccggcg tggctttggt cagcatcgga
    12361 gtttcgatgt cgaggaagcc gtggtcatcc ataaaacggc gcaccaggct ggtgatttta
    12421 gcgcgggttt tcaggcgctg cgccatttcc gggcgacgca ggtcgaggta gcggtatttc
    12481 agacgcgctt cttcggtgtt gacgtggtta gagtcaagcg gcagaacatc tgcgcggttg
    12541 atgatagtca gcgaggacgc cagcacttca atttcgccgg tcgccatatc gcggttaata
    12601 tttttttcgt cacgcgcacg tacggtgccc gtaacctgaa tgcagaactc attacgcagt
    12661 tcagaggcca gctttaacgc gtccgcacga tccggatcga aaaatacctg cacgatacct
    12721 tcgcggtcgc gcatatcgat gaagatcagg ctaccaagat cacgacgacg gttgacccaa
    12781 ccacacagag tcacctgctg ccccacgtgg gacaaacgga gctgtccaca atattctgta
    12841 cgcatgagat atcccttaac ttagctgccg gcggatgccc cctgctgcgc aggtgaccaa
    12901 gtcgcagcgt tagctgtatg tcacaactga atgaaaaaag gcggctatta tactggaaat
    12961 tctgccgcac cgtaagagcc tggcccgcgc tgtaacgcct cgttaccact ttatatcggg
    13021 cctgaaatca gactctacgc cagtttgcta taaaggtgtt gcccgaactc ataaaaatta
    13081 acaaaatttg tcgttccgcc atcggctaat cgcattaagg tgtgaggcac gattttgttt
    13141 tttcaggagt cagcatgctt gaacttaatg ctaaaaccac tgcgttggtg gtgatcgatt
    13201 tacaagaagg catcttacct tttgccggtg gtccacatac tgccgatgag gtagttaatc
    13261 gcgccgggaa gctggcggcg aaatttcgcg ccagcggtca gcccgtgttt ctggtgcgag
    13321 ttggctggtc tgccgattac gccgaagcat taaaacagcc ggttgatgcc ccctcccccg
    13381 ctaaagtgtt gcccgaaaac tggtggcaac atccagctgc attaggtgca actgacagtg
    13441 atatcgaaat catcaaacgt cagtggggtg cgttttacgg tacggatctg gagttgcaac
    13501 tgcgccgccg aggtatcgat acaatagtgt tatgtgggat ctcgaccaat atcggtgttg
    13561 aatccaccgc ccgcaatgcc tgggaacttg gctttaatct ggtgattgcc gaagacgcct
    13621 gtagtgccgc cagcgccgag caacacaata acagcattaa tcatatctac ccgcgcatcg
    13681 cccgtgtgcg tagcgtggaa gagatccttc acgcgttatg atttacatcg gtctaccgca
    13741 atggtcgcat cctaaatggg tgcggttggg gatcaccagc cttgaagagt atgcccgcca
    13801 ctttaactgc gtgatacggg atttttaaaa atcactaaag aacgcccaag agcatgtgtt
    13861 ttctttagaa taatcaaagc gttggattgc catgccatat gaaaatcggc aaagttcata
    13921 tgtaacaaag tgtcccaatc atgccccaaa atgccccaca acaaatattt ttgccccatg
    13981 catgccccaa aaaatcactt tccctcactc ttgacactgt ttatccatac agttaaaaat
    14041 aatactgtat aaaaacacag tgtagaggga tttttatgcg tattgaaatc tgcatagcca
    14101 aagagaaaat gactaaaatg ccaaccggtg ctgtggatgc gttaaaggaa gaattaaccc
    14161 gacgcatcag taaacgttat gacgatgtag aggtgatcgt aaaagccacc agcaacgatg
    14221 gcctttctgt tacgcgcacc gccgataaag attcagctaa aacttttgtt caggaaactc
    14281 tgaaagatac ctgggagtct gctgacgagt ggtttgttca ctaattagca cgtaaaatca
    14341 gtaacggctg gaaatcattc aatactcgca ctatcggaag ttcaccagcc agccgcagca
    14401 cgttcttgca tacgacgtgt ctgcggtctc aactccgccc gcccccgcca atcatgattg
    14461 ggcagtacca gtacagcact agcaaagaca ggaaattagc agtctcagca aaacaccgac
    14521 cagacggtga gaagacaaaa agatacgcaa agatgccgcg gttccagaag aataaaaaac
    14581 cgctaatgga cagcctcctt aacacagttt tttatataca tgattcactg ataccagtgg
    14641 cattaatagc tattatatag cttcagctcc ttttttcata ccttaaggaa aaattgcccc
    14701 ctcccccaaa aagtatcgtt tttttacatt aaaccgatcc agtttaggat agtgtaaagc
    14761 agaagttctt caatatataa catatggagt taatatgctt acacaaacta tatattcgcc
    14821 tcctccagaa gttcctgtct actgctgaag ggaaccaata acattgcaaa gcagataaaa
    14881 aagaatccaa cacctctcaa cataaagaat acgccctgat atttatgctc ccgacaaata
    14941 acaatcacag attaatttca aattcgttct ccacttattc aatcgacact agccgtgcat
    15001 atgaaaatta tctaacccat tggactgaat ggaaaaataa ccgcatacaa gaagaacaac
    15061 gagacatcgc ttttcagcga ctagtatcat gtctacaaaa ccaagagacg aacctggact
    15121 tgtctgaatt aggcctgaca acattacctg aaatcccccc ggaaattaaa tcaattaata
    15181 taagtaaaaa taatttaagc ttaatctccc cattgcctgc gtcccttaca cagcttaatg
    15241 tcagctataa cagacttatt gaactgcctg ctttgcctca aggacttaaa ttattgaatg
    15301 cgtcccacaa tcaactaatc acactaccca cactccccat atctttgaag gagcttcatg
    15361 tctcaaataa tcaattatgt tctcttcctg ttttaccaga actactggaa acattagatg
    15421 tatcatgtaa tgggctggca gttttaccac ctttaccatt ttctttacaa gagattagcg
    15481 caatagggaa tcttcttagt gaactccccc ctctacctca caacattcac tccatatggg
    15541 caatcgacaa tatgttaacc gatattccat acctgccgga aaatttaagg aacggttatt
    15601 ttgacataaa tcagataagt catatcccgg aaagcattct taatctgagg aatgaatgtt
    15661 caatagatat tagtgataac ccattgtcat cccatgctct gcaatccctg caaagattaa
    15721 cctcttcgcc ggactaccac ggcccgc
//
LOCUS       DAJVRF010000091        15582 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJVRF010000091 DAJVRF010000000
VERSION     DAJVRF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15582
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.091"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..2065
                     /locus_tag="OQN13_002667"
     CDS             <1..2065
                     /locus_tag="OQN13_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS1615983.1"
                     /translation="GYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSG
                     LNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVR
                     FRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDL
                     QLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSD
                     FVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDV
                     FDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDI
                     ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRR
                     ISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASN
                     NTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAG
                     ASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTP
                     YRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPL
                     TFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESR
                     NYICQ"
     gene            2081..3115
                     /locus_tag="OQN13_002668"
     CDS             2081..3115
                     /locus_tag="OQN13_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HCS1615984.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFML
                     ESNGSPVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(3197..3535)
                     /gene="rcnB"
                     /locus_tag="OQN13_002669"
     CDS             complement(3197..3535)
                     /gene="rcnB"
                     /locus_tag="OQN13_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCS1615985.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3754..4578)
                     /gene="rcnA"
                     /locus_tag="OQN13_002670"
     CDS             complement(3754..4578)
                     /gene="rcnA"
                     /locus_tag="OQN13_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCS1615986.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4699..4971
                     /gene="rcnR"
                     /locus_tag="OQN13_002671"
                     /pseudo
     CDS             4699..4971
                     /gene="rcnR"
                     /locus_tag="OQN13_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
     regulatory      5063..5159
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5194..5982
                     /gene="thiM"
                     /locus_tag="OQN13_002672"
     CDS             5194..5982
                     /gene="thiM"
                     /locus_tag="OQN13_002672"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCS1615987.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5979..6779
                     /gene="thiD"
                     /locus_tag="OQN13_002673"
     CDS             5979..6779
                     /gene="thiD"
                     /locus_tag="OQN13_002673"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCS1615988.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6844..7662
                     /locus_tag="OQN13_002674"
     CDS             6844..7662
                     /locus_tag="OQN13_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCS1615989.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7714..8460
                     /locus_tag="OQN13_002675"
     CDS             7714..8460
                     /locus_tag="OQN13_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1615990.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8434..9399)
                     /locus_tag="OQN13_002676"
     CDS             complement(8434..9399)
                     /locus_tag="OQN13_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1615991.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9396..10400)
                     /locus_tag="OQN13_002677"
     CDS             complement(9396..10400)
                     /locus_tag="OQN13_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCS1615992.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10397..11674)
                     /locus_tag="OQN13_002678"
     CDS             complement(10397..11674)
                     /locus_tag="OQN13_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1615993.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11931..12983
                     /gene="fbaB"
                     /locus_tag="OQN13_002679"
     CDS             11931..12983
                     /gene="fbaB"
                     /locus_tag="OQN13_002679"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCS1615994.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13302..14156
                     /gene="gatY"
                     /locus_tag="OQN13_002680"
     CDS             13302..14156
                     /gene="gatY"
                     /locus_tag="OQN13_002680"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCS1615995.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14185..15442
                     /gene="gatZ"
                     /locus_tag="OQN13_002681"
                     /pseudo
     CDS             14185..15442
                     /gene="gatZ"
                     /locus_tag="OQN13_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15452..>15582
                     /locus_tag="OQN13_002682"
     CDS             15452..>15582
                     /locus_tag="OQN13_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCS1615996.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAR"
BASE COUNT         4088 a         3728 c         3987 g         3779 t
ORIGIN      
        1 tggctatgtt ccaccggaaa actgggagcg gggtattaat gcgttttata cctcttatta
       61 tgtgagtcag tattacagtg actataaagc gtcgggtaat agtaagagta catatgtacg
      121 ttttaacagc gggttaaatt tactggggtg gcaactgcat tctgatgcca gtttcagtaa
      181 aacaaataac aatccagggg tgtggaaaag caataccctg tatctggaac gtggatttgc
      241 ccaacttctc ggcacgcttc gcgtgggtga tatgtacaca tcaagcgata tttttgattc
      301 tgttcgcttc agaggtgtgc ggttgtttcg tgatatgcag atgttgccta actcgaaaca
      361 aaattttacg ccacgggtgc aggggattgc tcagagtaac gcgctggtaa ctattgaaca
      421 gaatggtttt gtggtttatc agaaagaggt tcctcctggc ccgtttgcga ttacagattt
      481 gcagttggcc ggtggtggag cagatcttga tgtcagcgtg aaagaggcgg acggttcggt
      541 aaccacctat ctggtgcctt atgcagcggt gccaaatatg ctgcaacccg gcgtgtcgaa
      601 atatgatttt gcggcgggtc gtagccatat tgaaggggcg agcaaacata gtgattttgt
      661 ccaggcgggt tatcagtatg gttttaataa cttattgacg ttgtatggtg gctcgatggt
      721 tgcgaataat tattacgcgt tcactttggg gactggctgg aatacacgca ttggtgccat
      781 ttccgtcgat gccacgaagt cgcatagtaa acaagacaac ggcgatgtgt ttgacgggca
      841 aagttatcaa attgcctaca acaaatttgt gagccaaacg tcgacgcgtt ttggtctggc
      901 ggcctggcgt tattcgtcgc gtgattaccg gacatttaac gatcacgttt gggcaaacaa
      961 taaagataat tatcgccgtg atgaaaacga tgtctatgac attgccgatt attaccagaa
     1021 tgattttggt cgcaaaaata gcttttccgc caatatgagc cagtcattgc cagaaggttg
     1081 ggggtctgtg tcattaagta cgttatggcg agattactgg gggcgtagcg gcagtagtaa
     1141 ggattatcag ttgagttatt ccaacaacct gcgacggata agctataccc tcgcggcaag
     1201 ccaggcttat gacgagaatc atcatgaaga gaaacgtttt aatattttta tatcgattcc
     1261 ctttgattgg ggtgatgacg tttcgacgcc tcgtcggcaa atatatatgt ctaactcaac
     1321 gacgtttgat gatcaggggt ttgcctcaaa taatacggga ttatcaggaa cagtagggag
     1381 tcgggatcag ttcaattatg gtgtcaacct gagtcatcaa catcagggaa atgaaacgac
     1441 agctggagcg aatttaacct ggaacgcgcc ggtggcaaca gtgaatggca gttatagtca
     1501 gtcgagtact tatcgacagg ctggagccag tgtttcaggg ggcattgtcg cctggtcggg
     1561 tggcgttaat ctggcgaacc gtctttccga aacgtttgct gtgatgaatg cgccaggaat
     1621 taaagatgct tatgtcaatg ggcaaaaata tcgcacaaca aaccgtaatg gagtggtggt
     1681 atacgacgga atgacacctt atcgggaaaa tcacctgatg ctggatgtgt cgcaaagcga
     1741 tagcgaagca gaattacgtg gcaaccggaa aattgccgca ccttatcgcg gcgcggttgt
     1801 actggttaat tttgataccg atcagcgcaa gccctggttt ataaaagcgt taagagcgga
     1861 tgggcaacca ttaacgtttg gttatgaagt caatgatatc cacggtcata atattggcgt
     1921 tgtcggccag ggaagccagt tatttattcg caccaatgaa ataccgccat cggttaatgt
     1981 agcaattgat aagcaacaag gactttcatg cacaatcacc ttcggtaaag agattgatga
     2041 aagtagaaat tatatttgcc agtaatagtc aggtggttct atggaaattc gcataatgct
     2101 atttatatta atgatgatgg ttatgcctgt gagctatgcg gcatgttata gtgagttatc
     2161 tgttcagcac aatttggttg ttcaggggga ttttgcactt actcaaacac aaatagcgac
     2221 atatgagcat aattttaatg attcgtcatg cgtgagtaca aatactatca cccctatgag
     2281 ttcgtcggat attattgttg ggctttataa cgataccata aaattaaatt tacattttga
     2341 atggaccaat aaaaacaaca tcacgttgtc aaataatcag accagtttca ccagtggtta
     2401 ttcagttacg gtgacacctg cggccagtaa tgcaaaagtg aatgtttctg cggggggcgg
     2461 cggttcagtg atgattaatg gtgttgcgac attatccagt gcttcatcat cgacacgcgg
     2521 gagtgccgca gtacaatttc tactgtgttt attaggtggc aagtcatggg atgcatgtgt
     2581 aaatagctac agaaatgcat tggcacaaaa tgcaggtgtc tattccttta atctgacatt
     2641 gtcatacaac ccgataacca caacctgcaa accggacgat ttattaatta ctttagacag
     2701 tattcccgtt tcacaattac cagccacagg taacaaagca acaataaata gtaaacaagg
     2761 ggatattatt ctgcgttgta aaaatttatt aggtcaacaa aatcaaacat cacggaaaat
     2821 gcaggtgtat ttatcaagtt ctgatttgtt agccggcagt aacacgatcc tgaaaggtgc
     2881 agaaaataat ggagtcggat ttatgcttga aagcaatggt tctccagtca catttttaaa
     2941 tatcactgat aacagcaaag gatacacaaa tttaaaagaa attgcggcaa agtcaaaact
     3001 tacagataca acggtttcaa ttccgataac tgccagttac tacgtctacg atacaaacaa
     3061 agttaaatct ggcgcactgg aggcaaccgc attaatcaac gtgaaatacg actaataaca
     3121 agcaagacga gcaagtggct aataataaaa aataacaagg tgtctgcatt tccccctcat
     3181 gatgaggggg ctttttttag cgatgataaa aaatctcacc gtcgtaggct ttaatgattt
     3241 taccgtcggt gtcgctgatc aacacgtacg cgccacccat ataggtccag tgcgtcccgg
     3301 catcaggcgc gggcaggtta cgtaactgcc actgcttaat gttgtactca tcggtgcggt
     3361 acatctctgg gacggtatca cctggtttga aatgcttaaa gtcagcaatg aaactactta
     3421 actcatactt agaaattccc gaggtattgg tcgaacccgc ggtggctggt gcggcccagg
     3481 cggcactggt aaccagcaaa agcacaccca gcaacatctt atttttaatg gtcataaact
     3541 ctccactttg gggatctgta atatcagtcg catatctttc ctgccataca atataactga
     3601 caagcgatat tttgttagtg atcccatgat ttcgtaaaaa gtgtaaatga aaggatgccc
     3661 taaaagcatc cttagcgagc agagagtcag cggaaatagc tgatttaaga atcatcctgg
     3721 gctgaagata acatgcaagc ctaaaggatt gagttatcgc attatgccca tgaagccatg
     3781 tacgcccata tacacaccga ctaagccaat caacagactg gaaaaatagg gggcgcgttt
     3841 agcgagagtg ttaaatccgc tccagcgttt tgcgacctgc tgaacgctga ttgctgcgcc
     3901 aacgcctacg gtgacaagcg ttaacgccag gccaatgctg aaactgacga ccagtgttgc
     3961 gcccagtgtc agggctttca actgaatgca tatcaacagc acggtaattg ctgccgggca
     4021 ggggataagg ccaccggtta agccaaatag caaaatttgc cagttggtga cctctctacc
     4081 atcaaagcgt cgtttaatgt cattggcatg ggctcgtgca tgggcatcct gatactcgcc
     4141 atgttcgtga tggtgatgat gtccatggtc gtggtgatct tcgtgatcgt gatgatgatg
     4201 ctcataatca tactcgtgca tattctccag ccagttgcgt tcgccgcgcc aggtacgcca
     4261 gaacatccag aacgcggtgc caataatgat cactgcggaa atcagctgga gccacggttc
     4321 tgctgattga gcagtaaagc gcttgctgat caccatcccg ccaaaggcaa ttaaccagac
     4381 cactgcggta tgcgaaatag ttgctgccag tccgagcatc accgcttgtt taatggtgcc
     4441 tttgatggcg atgataaacg ccgccatcat cgtttttgag tgccctggtt ccaggccatg
     4501 aagcgcacca agtaagatgg cgctggggat gaagaaccag gcgtttcctt gctgaagaag
     4561 agttgtaaat tcggtcatga taataattct tagtattaat tcggcaatct gattctactc
     4621 cccccagtac ctgatactac cccccagtag attaatagtg ctatgatatt ttcatattct
     4681 tgttaaccag gtgttgccat gtctcataca atccgtgata aacagaaact gaaagcgcgt
     4741 gccagtaaga ttcagggcta ggtcgtggcg ctcaagaaaa tgctcgacga gccgcatgaa
     4801 tgcgctgcag ttttacaaca gattactgct atccgtggcg cggtaaacgg tctgatgcgg
     4861 gaagtgatta aaggtcatct gacggaacac atcgttcacc agggggatga gctaaaacgt
     4921 gaagaagatc tggatgtcgt tctgaaggtg ctggattcat atatcaaata atttattaac
     4981 gcgattgtaa aactgccgtt tttcctcgtt tacaacgcgt gcgctggaca ttaccatcct
     5041 cctctgcgat ttatcatcgc aaccaaacga ctcggggtgc ccttctgcgt gaaggctgag
     5101 aaatacccgt atcacctgat ctggataatg ccagcgtagg gaagtcacgg accaccaggt
     5161 cattgcttct tcacgttatg gcaggagcaa actatgcaag tcgacctgct gggttcagcg
     5221 caatctgcgc acgcgttaca cctttttcac caacattccc ctcttgtgca ctgcatgacc
     5281 aatgatgtgg tgcaaacctt taccgctaat accttgctgg cgctcggtgc atcgccagcg
     5341 atggttatcg aaaccgaaga ggccagtcag tttgcggcta tcgccagtgc cttgttgatt
     5401 aacgttggca cactgacgca gccacgcgct caggcgatgc gtgctgccgt tgagcaagca
     5461 aaaagctctc aaacaccctg gacgcttgat ccagtagcgg ttggtgcgct cgattatcgc
     5521 cgccattttt gtcatgaact tttatctttt aaaccggcag cgatacgtgg taatgcttcg
     5581 gaaatcatgg cattagctgg cattgctaat ggtggacggg gagtggatac cactgacgcc
     5641 gcagctaacg cgatacccgc tgcacaaaca ctggcacggg aaactggcgc aatcgtcgtg
     5701 gtcactggcg aggtggatta tgttaccgat ggccatcgcg ccgttggcat tcacggtggc
     5761 gatccgttaa tgaccaaagt ggtaggaact ggctgtgcat tatcggcggt tgtcgctgcc
     5821 tgctgtgcgt taccaggcga tacgctggaa aatgtcgcat ctgcctgtca ctggatgaaa
     5881 caagccggag agcgcgcagt cgccagaagc gaggggctag gcagttttgt tccacatttc
     5941 cttgatgcgc tctggcaatt gacgcaggag gtgcaggcat gaaacgaatt aacgctctga
     6001 cgattgccgg tactgatccg agtggtggtg cggggattca ggccgatctt aaaaccttct
     6061 cggcacttgg cgcttatggt tgctcagtta ttactgcact ggtggcgcaa aatacccgtg
     6121 gcgtacagtc ggtgtatcgc attgagcctg attttgtcgc cgcccagctc gattcggtgt
     6181 tcagcgatgt gcgaatcgat accactaaaa tcggtatgtt ggcggaaacc gatattgttg
     6241 aagcggtggc agaacggttg caacgttatc agatccaaaa cgtggtactc gacaccgtta
     6301 tgctggcaaa aagcggcgac ccactgcttt caccttcggc agttgccacg ctacgcagtc
     6361 gattattgcc acaggtttca ttaataacgc caaacttgcc cgaagcagcc gccttgctcg
     6421 acgcgccaca cgcgcgcacc gaacaggaaa tgctggaaca agggcgatcg ctgttggcga
     6481 tgggctgtgg cgcagtgcta atgaaaggtg gtcatctgga tgatgagcaa agcccggact
     6541 ggctgtttac ccgcgagggt gaacaacggt ttaccgcacc gcgcattatg accaaaaaca
     6601 cccacggtac tggttgtaca ctctctgcgg cgttggctgc actacgcccg cgccatacaa
     6661 actgggctga caccgtacag gaggcaaaaa gctggctttc atcggcgtta gcccaggccg
     6721 acacgctgga agttggtcac ggtattggtc cggttcacca cttccacgcc tggtggtgag
     6781 caccgtacta tagctgcttt ggttattgtc agcctttggc actgacattc agggatgaaa
     6841 ccaatgcagc taagaattac cagtcgaaaa aaactcaccg ctctattatg cgctcttggg
     6901 ctaatttcca tcgttgcaat ttatccgcgt cagacggtga attttttcta ttcgacagca
     6961 gttcagatta cagactacat ccacttctac ggttatcgcc cggttaaatc tttcgctatt
     7021 cgtattcctg ccagttacac cattcacgga atagatgttt cgcgctggca ggagcggatc
     7081 gactggcagc gtgtggcaaa aatgcgcgat aacggtattc gcttacaatt tgcctttatt
     7141 aaggcgacgg aaggcgaaaa gctggtggac ccctattttt cgcgtaactg gcaactaagc
     7201 cgcgaaaatg gcttgctgcg cggggcatat cattatttct ccccgtcggt agcagcaccg
     7261 gttcaggtgc gattatttct gcaaacggta gatttctcac aaggcgattt ccctgcggta
     7321 ctggacgtag aagagcgagg aaaattatcg gcaaaagaat tacgcaagag ggtaagtcag
     7381 tggctaaaaa tggtcgaaaa aagtacgggg aaaaagccga ttatttactc aggagccgtt
     7441 ttttatcaca cgaatctggc gggctatttc aatgaatatc cgtggtgggt ggctcactac
     7501 tatcaacgtc gtccggacaa tgacggcatg gcctggcgct tctggcagca ttccgaccgt
     7561 ggacaggtag atggcatcaa tggtccggtg gattttaatg tgtttaatgg cacggtggag
     7621 gagttgcagg gattcgttga tgggattaaa gaaacgcctt aaataagaag cagtgatata
     7681 ctcccaccag actccctggc aggaaaaaga caaatggaac aagcgcatac ccagctgatt
     7741 gcccaactga acgaacgtat tttagcggcg gataacacgc cgctttatat taagtttgcc
     7801 gaaacggtaa aaaatgccgt gcgcagcggg gtgctggagc atggcaatat tttgcccggt
     7861 gagcgtgatt taagtcagtt aaccggcgtt tcgcgcatta cggtgcgcaa ggcgatgcag
     7921 gcgctggaag aagagggcgt ggtgacgcgt tcgcgcggtt acggcacgca gatcaacaac
     7981 atcttcgaat actcgttgaa agaagcgcga ggtttttctc agcaggtggt gttgcgcggg
     8041 aaaaagcccg atacgctatg ggttaacaag cgtgtcgtga aatgtcccga agaagtcgcg
     8101 cagcagctgg cggtcgaagc aggaagtgat gtctttttgc ttaagcgtat tcgctatgtc
     8161 gatgaagaag cggtatcgat tgaggaatcg tgggttccgg cgcatttaat tcatgatgtt
     8221 gatgccatcg ggatttcact ttatgactat ttccgcagcc agcacattta cccacaacgt
     8281 acgcgttccc gcgttagcgc ccggatgccg gatgccgagt ttcagtcaca tattcagtta
     8341 gatagcaaaa taccggtgct ggtgatcaag caagtggcgc ttgaccaaca gcaacggcct
     8401 attgagtaca gcattagcca ctgtcgcagc gatctatacg tttttgtgtg cgaggagtag
     8461 ttcctcgcgc gttggcgcac aatcaccgcc ccgatgcccg acaacccacg acgccactgc
     8521 attgcccagt aatacggcat ccgccagtgg cagaccagag gccagcccgg caagtacgcc
     8581 acctgcatga ctgtcgcccg ccccaatggt gtctacaact tgcgttggaa atgccggaat
     8641 gcagccagaa gcgtcgttgc tgaaatacca tgcgccttct ttatcgagac gaacgatcaa
     8701 cggcgcggca aatttctcct gccattgctc gccaagtgtt gttatctctg cggataaagc
     8761 aaaacgttcg gcggcaatct cagcctcttg acgattgagc gacactaaag gtcgacaggc
     8821 catgatccgc gccagtaatg catccgggat atcgccaata cgtgggccaa aatcgataaa
     8881 cggcgtcacg tcttgcagct cttccagcca ttccactaac aattcgccgc agggcgaggc
     8941 cagttgataa ccggaaaaat agagcaggct gccaggcgca acggttaatc gcgccagcca
     9001 ctggcgattc cactgatttt ccacaccgct gaatgacata aaagtgcgtt cgccatccgg
     9061 ctcaaccagc gccagacacc agccgttatc accttcggcg ttatcgatca ggctgattaa
     9121 gccctctttt gccatccggt tgcgaataat ctccgcccac acgccctgac cgagcggcaa
     9181 ggcattaccc gcttcgatgc cgaggcgctt taacgccacg gcaatattca gtgcgcagcc
     9241 gccaacgtta acgctctgct gtttcagttc gatatcgcac ccacgccagg ggagggcata
     9301 agcgtcggcg atcacgtcaa tgaccgccgc gccgacgacc atcaccggct gacgcgtggt
     9361 taattcaggc agcagcgtgt gtaatcgagc gccgctcata ccgcctcccg ttgttgacga
     9421 tatttcgcca gcgctgtggc atagcggttg aaatcaagct gatttaccgc atccagttcc
     9481 gcctttaacg caggatcgat agcgttaacg ccatgcaacg cgccgcaaat tgccgtcgcc
     9541 atagcaccga tggtgtctgt gtcgccgcca aggttagcgc acaggacggc gcagcgattc
     9601 gggtcggttt gtgctagttc aaccagcgca atggcgcacg gaacggactc aatagtgctg
     9661 gtacctgcgc caacgacctg gtaaagctgt tcgctggcgg attcggtgcc gtcggcattg
     9721 cgcacaattt tcagcgcaat ctccagccgc gccgccagtg aggcgctgaa cgtagtgatg
     9781 cgtttttgct gtgcatgtcg cgcaattgaa ggcagggaat caacaatcgc tgaccagctt
     9841 tctccgtcaa tggcacgaga aatcgcccat gcgatgacta ccgcgcctgc aaccgccaga
     9901 tcggatttat gggtcgggct ggacgccagc gctacatcat caataaagga atcaacatca
     9961 cgcgccggaa gcaaacaacc taatggcgag acgcgcatcg ctgcgccgtt ggtcacgccg
    10021 ttgttttcca gttcagcaac gggtttaccg tcgcgaatgg cgttaagcgc aatcttcgag
    10081 gtcggaccta gtacgttttt gttaaaggcg tcgaaacgca gcgcccagtc gagaatatta
    10141 cgcccaatca gatccggatc gatcttgcct tcacgttcca gtaacgcatc cgccagacac
    10201 agcgccatcg aggtatcgtc ggtgaattcg gcgcggttaa aataacaggc cgcgttattc
    10261 tcctttggtc caggaagaaa acggtcaatc cagccaaagt gtgctttaac gcggctgcgt
    10321 ggccaaagct cggagggcat ccccatcgca tcccctaacg cctgcccata aagagcaccg
    10381 agaatacgtt ctgttttcat ttaacttccc cttgtgtcaa cgcaatatcg cgatcatcga
    10441 ccttgatagc cgtaatttcg ttgtcggatt cgcggaaaaa aatcatgaac agcacggcga
    10501 taatggcaat catcaccgcg ccgaaagtcc acatcccgga ccagttgaaa gtcagtccgt
    10561 ttaccggttc ctgataagcg aacatctttt ccatcatcac accgccaaga cgatagccga
    10621 gcaaactgcc gaagccctgg cagcagagcg tgatcagccc ctgcgcagcg gtacgcatat
    10681 gcacgggggc ttttttatcg acatagatgt aagcggtaac gtagtaaaaa tcgtaactta
    10741 cgccgtgaag caaaataccg aggaacagta acgcgtaggt gaaatattca tccgtactac
    10801 cgtaaataaa gaagccatag cggatcgcag cggtgaccag accaagcaat aatacctttt
    10861 tgataccaaa gcgtttagtg aaaaacggca atgccagcat aaagaagatt tcagagaact
    10921 ggccgagcgt catccagccg gtggcgtttt tcatgccaac ttcggtcaga taaccgttgg
    10981 caaagatgta atagaacgcc agtggcatcg caaacaggaa tgaacagaag aaaaagacga
    11041 ggaagttttt atcgcgcagc aggatcagcg catccaggcc gagcatgact ttaatatcca
    11101 ttttgccggt gctttttggt ggcgtgtcgg gcaggaaaaa cgcaaacaca ccgagcagag
    11161 cagaacttcc ggcggtaatc agcagcggga tgttagtcgg tgagatatcg gcatacccca
    11221 gtatttgcgg caagaaacca catgccagac cggaggcaat ccagccgata gtgcccatca
    11281 cacgaatgcg cgggaaatca cgctccacat ccggcacgtt ggcaaaagcg atgctgttag
    11341 tcagcgcaat ggtcggcata taggttagag agtaagccag cagtaacgga aagaacccgg
    11401 caaaagtggt ctgttgcgca gcgaaataca tcagcagcgc gcctgcgaac atcaataccg
    11461 ccagcacttt ttgcgccgag aaaaagcggt cagtgatgga gccaaccaga attggcgaca
    11521 ggatcgccgc aatggcggta caggcatacg accagccaat ttctccggca ctaaaaccgc
    11581 ttttacttaa ccacaaccac aatggcacaa accacgcgcc ccagataaac cattcaacaa
    11641 acatcatgaa cgacagcttt gctgttgttt tcatctttta attccttcat gacaggtaat
    11701 gtaaggtacg cgatgacaat accacttaac aatacctttt aaataccttt gcggtgaaaa
    11761 cttgaccgcg ctaacatttt ttctgatgaa tcgagccaac agaaaacgct gaaaaaacat
    11821 ccaaaagatg gaaaaactcg cccgtaacga ccaggattat gattgcccgc aaaaggcggg
    11881 ccaggacaat ccatagccga tatccaatcg gaatttacgg gagcatagta atgacagata
    11941 ttgcgcagtt gcttggcaaa gacgccgaca accttttaca gcaccgttgt atgacaattc
    12001 cttctgacca gctttatctc cccggacatg actacgtaga ccgcgtaatg attgacaata
    12061 atcgcccgcc agcggtgtta cgtaatatgc agacgttgta caacaccggg cgtctggctg
    12121 gcacaggata tctttctatt ctgccggttg accagggcgt tgagcactct gccggagctt
    12181 catttgctgc taacccgctc tactttgacc cgaaaaacat tgttgaactg gcgatcgaag
    12241 cgggctgtaa ctgtgtggcg tcaacttacg gcgtgttggc gtcggtatcg cggcgctatg
    12301 cgcatcgcat tccatttctc gtcaaactta atcacaacga gacgctaagt tacccgaata
    12361 cctacgatca aacgctgtat gccagcgtgg agcaggcctt caacatgggc gcggtggcgg
    12421 ttggtacgac tatctatttt ggctcggaag agtcacgtcg ccagattgaa gaaatttctg
    12481 cggcctttga acgcgcgcac gagctgggca tggtgaccgt gctgtgggcc tatttgcgaa
    12541 actccgcctt taagaaagat ggcgttgatt accatgtttc cgccgacctg accggtcagg
    12601 caaaccatct ggcggcaacc atcggtgcag atatcgtcaa acaaaaaatg gcggaaaata
    12661 acggcggcta taaagcaatt aattacggtt acaccgacga tcgtgtttat agcaaattga
    12721 ccagcgaaaa cccgattgat ctggtgcgtt atcagttagc taactgctat atgggccggg
    12781 ctgggttgat aaactccggc ggtgctgcgg gcggtgaaac tgacctcagt gatgcagtgc
    12841 gtactgcggt tatcaacaaa cgcgctggcg gaatggggct gattcttgga cgtaaagcgt
    12901 tcaagaaatc gatggctgac ggcgtgaaac tgattaacgc cgtgcaggac gtttatctcg
    12961 atagcaaaat tactattgcc tgatacgcct catacgaaca gcaccacccc gtaggacgga
    13021 taaggcgttt acactgcatc cggcaaccta tgcctgatgc gacgcggaag cgtcttatca
    13081 tgcctacata gcactgccac gtatgtcgga aaaggcgttt acaccgcatc cggcataaaa
    13141 acacgcgcac tttgctacgg cttccctatc gggaggccgt ttttttgcct ttcactcctc
    13201 gaacagtttt catattgtcg ttttgtgatc gttatctcga catttaaaaa caaatcattt
    13261 cattatattt tgaaatcgaa aacaagcaac aggatatgaa aatgtacgtg gtatcgacaa
    13321 agcagatgct gaacaacgca cagcgcggcg gttatgcggt tccggcattc aatattcaca
    13381 atctcgaaac gatgcaagtg gtggtggaaa ccgctgccaa cctgcatgcg ccggtcatca
    13441 tcgccggaac gcctggcaca ttcacttatg ctggtacaga aaatctgttg gcgctggtca
    13501 gcgcgatggc gaagcaatat caccatccac tggcaattca tctcgaccat cacacgaaat
    13561 ttgacgatat cgctcagaag gttcgttctg gcgtgcgctc agtcatgatt gacgcctcgc
    13621 atttgccttt tgcgcaaaat atttcacggg tcaaagaggt ggtggatttt tgccatcgct
    13681 ttgatgtcag cgtcgaagcg gagctggggc aacttggcgg ccaggaagat gatgtgcaag
    13741 tcaatgaagc cgatgttttt tataccaacc ccgctcaggc gcgtgaattt gccgaggcaa
    13801 ccggaattga ttccctggcg gtcgccatcg gcacggctca tgggatgtat gccagcgcac
    13861 cggcgcttga tttttctcga ctggagaaca ttcgccagtg ggtgaactta ccgctggtgc
    13921 tgcatggcgc gtcagggtta tcgactaagg atattcagca aaccatcaaa ctggggatat
    13981 gcaaaatcaa cgttgcaacg gagctgaaaa atgccttctc gcaggcgtta aaaaattacc
    14041 tgaccgagca ccctgaagcg accgatcccc gggattattt gcagtcggct aaatccgcaa
    14101 tgcgcgatgt ggtgagcaaa gtgattgccg attgtggctg cgagggcagg gcataacgca
    14161 cctgccattt aacaaggaaa aaacatgaaa acgttaattg cccggcataa agctggtgaa
    14221 catatcggca tatgttcagt ctgttctgcc catccgttgg ttatcgaagc ggcgctggca
    14281 tttgatcgca acagcacgcg caaagtgctg attgaagcaa cgtcaaacca ggtcaatcaa
    14341 tttggcggtt ataccggaat gacaccggca gactttcgcg aatttgtttt tacgattgcc
    14401 gataaagtcg gatttgcacg cgaacgcatt attctcggcg gcgatcatct ggggccaaac
    14461 tgctggcagc aagaaaatgc ggatgcggcg atggaaaaat ccgtcgagct ggtaaaagca
    14521 tatgttcgtg ctggcttcag taaaattcat cttgatgcgt caatgtcctg cgcgggggat
    14581 cccataccgt tagcaccaga aacggttgcg gaacgagctg ctttgctttg ctgcggaaag
    14641 tgtggcgaca gattgccagc gtgagcaact gagctatgtc attggcactg aagttccggt
    14701 tccgggcggt gaggccagcg ccattcagtc agtacacatc acccatgttg aagatgccgc
    14761 caatacttta cgtacgcatc aaaaggcctt tattgcccgt gggctgacag aggcgttaac
    14821 acgcgtgatt gccatcgtgg tgcagccggg cgtggaattt gatcacagca atattatcca
    14881 ttatcagccg caggaagcgc agccgctggc gcaatggata gaaaacaccc gaatggttta
    14941 tgaagcacat tctaccgatt accagacccg gacggcttat tgggaattag tccgcgatca
    15001 ctttgcaata ttgaaagtcg gtcccgcatt aacctttgct ttacgcgagg cgatatttgc
    15061 actggcacaa attgagcagg aacttatcgc ccctgaaaat cgcagcggtt gcctggcggt
    15121 aattgaagaa gtgatgctcg acgaaccgca atactggaaa aaatattatc gtacgggttt
    15181 taacgattca ttactggata ttcgttacag cctgtcggat cgtattcgtt attactggcc
    15241 gcatagtcgg attaaaaata gcgtcgaaac gatgatggtg aatcttgaag gcgtggacat
    15301 cccactgggc atgattagtc agtatcttcc caaacaattt gaacgcattc agtccgggga
    15361 attatcagca ataccgcatc agctgattat ggataaaatt tatgatgttt tgcgcgccta
    15421 tcgctacggc tgtgcggaat aaggacggta tatgactaac ctgtttgttc gtagcggaat
    15481 ttcttttgtc gatcgtagcg aagttttaac ccatatcggt aatgagatgc tcgccaaagg
    15541 tgtggttcat gatacctggc cacaggcatt aattgccaga ga
//
LOCUS       DAJVRF010000092        15577 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJVRF010000092 DAJVRF010000000
VERSION     DAJVRF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15577
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.092"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..69)
                     /locus_tag="OQN13_002683"
     CDS             complement(<1..69)
                     /locus_tag="OQN13_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="periplasmic protein induced by stress response via
                     Cpx and BaeSR system; similar to CpxP; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCS1615997.1"
                     /translation="MRKLTALFVASTLALGAANLAHA"
     gene            complement(399..1367)
                     /gene="astE"
                     /locus_tag="OQN13_002684"
     CDS             complement(399..1367)
                     /gene="astE"
                     /locus_tag="OQN13_002684"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCS1615998.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1360..2703)
                     /gene="astB"
                     /locus_tag="OQN13_002685"
     CDS             complement(1360..2703)
                     /gene="astB"
                     /locus_tag="OQN13_002685"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCS1615999.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(2700..4178)
                     /gene="astD"
                     /locus_tag="OQN13_002686"
                     /pseudo
     CDS             complement(2700..4178)
                     /gene="astD"
                     /locus_tag="OQN13_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4175..5209)
                     /gene="astA"
                     /locus_tag="OQN13_002687"
     CDS             complement(4175..5209)
                     /gene="astA"
                     /locus_tag="OQN13_002687"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCS1616000.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5206..6426)
                     /gene="astC"
                     /locus_tag="OQN13_002688"
     CDS             complement(5206..6426)
                     /gene="astC"
                     /locus_tag="OQN13_002688"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCS1616001.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            6872..7678
                     /gene="xthA"
                     /locus_tag="OQN13_002689"
     CDS             6872..7678
                     /gene="xthA"
                     /locus_tag="OQN13_002689"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCS1616002.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            7845..8555
                     /locus_tag="OQN13_002690"
     CDS             7845..8555
                     /locus_tag="OQN13_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCS1616003.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8560..9237
                     /gene="ydjY"
                     /locus_tag="OQN13_002691"
     CDS             8560..9237
                     /gene="ydjY"
                     /locus_tag="OQN13_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCS1616004.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9252..9959
                     /locus_tag="OQN13_002692"
     CDS             9252..9959
                     /locus_tag="OQN13_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCS1616005.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            9959..10507
                     /locus_tag="OQN13_002693"
     CDS             9959..10507
                     /locus_tag="OQN13_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCS1616006.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQNPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10517..11683
                     /locus_tag="OQN13_002694"
     CDS             10517..11683
                     /locus_tag="OQN13_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1616007.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            11656..13192
                     /locus_tag="OQN13_002695"
                     /pseudo
     CDS             11656..13192
                     /locus_tag="OQN13_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            13192..13845
                     /locus_tag="OQN13_002696"
     CDS             13192..13845
                     /locus_tag="OQN13_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS1616008.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            13912..15219
                     /gene="ynjE"
                     /locus_tag="OQN13_002697"
     CDS             13912..15219
                     /gene="ynjE"
                     /locus_tag="OQN13_002697"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCS1616009.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKLPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15228..>15577)
                     /locus_tag="OQN13_002698"
     CDS             complement(15228..>15577)
                     /locus_tag="OQN13_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCS1616010.1"
                     /translation="FYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAAK
                     HQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRV
                     WSGYLTLKSLQRQ"
BASE COUNT         3646 a         4252 c         4058 g         3621 t
ORIGIN      
        1 ggcatgggcc aggttagccg cgccaagagc cagggtagag gcaacaaaca gtgcagttaa
       61 tttacgcata ttctatatcc ttcctttcag ttatttatta cggctttctt aagtagcgtg
      121 ccgtgttgac gagattaact ttactgactt tagcgtcaat taatcagagc aacggtaaaa
      181 caatgaaagt gtaaaaaaca cttttgcgcc aattatggag aaaaaaagaa aatttgatgg
      241 agagtgatga gagaatatta caacacaatg attttgcaga gattatgaag aactataccg
      301 gatgactggt gataaataaa gcaaataacc aggattaatc tgtattaatt tataagaaag
      361 caacttaata cccgcagaat gatttctgcg ggtaagtatt agcttatttt ttcgagcatt
      421 aatcccgcgc gtaatcccaa cgctaccaac ggattaggga ataacacata ctctacatca
      481 tgggttacgg taaaacgttc ctctccgtcc tgcgccagca atgttccttt ctcaaacggc
      541 ataaaattca gcgtgtcact tgccatatgc atttcgaagg acggcgagtg gcgagtaatt
      601 tgcgaaacca cccgataacg gagcggcggt gttctcacga taccgacact ctcaccagat
      661 agcagcgcag caattgcgct ggcagttact gcaaactggc gaagatcgtt ttgcccaaag
      721 ggcaacgctt tgccaagttc cagcgtacag gccagcgcgc caaaatgtct ggcgctgaaa
      781 tgggtaaacg taccaccagg ctcctgatgg aacaccagcg cctccagccc cgccgcaccc
      841 agccacgtca gaaatttctc gtcccaggga atgtcgcgtt gcggtaatac accgaactga
      901 ggatgcaagg agccacgaat tgcggtatgt agatcaaggt gccaacgcac agattctttg
      961 ccctggtcat aaaaatcttc caggcactgt tccagttcgc gcgcccgaca ggtttctccg
     1021 ctttcagcaa atagctgcca acgaccgcca aacattcggt tcatatcgct atggcaataa
     1081 cgtttccctt gcttcagcgc aggaggatcc ccgaggatca ccagcaaccg ccaacgtaac
     1141 gggatctcgc cgtgagatat cgcgccaagc aacgcgtcca gcatttccac aggtgccgtc
     1201 tcattaccgt ggatccccgc agaaatcacc agcgcgcctt gcggtggcgt taatggcgtc
     1261 agttccagca caccatcgcc cagccagcgc cagcgaacgc cgttgatttc tcgctcggtg
     1321 ataaccggtt ttttacccgt taaggtcaga gcaagaaaat tatccattgc cccctccctc
     1381 gcgctggaac ggataaaccg aaccgagatt cagtaattgg ctcaatacat ccagtgcttc
     1441 ccgcccttcg cgcagcaatt gcgggtcggc cagatcggca gcagtaaggc gatcgcggta
     1501 gtaacgatcc acccagtcat tgagcgcatt aaacagcgta tcgttcatca tcaccgccgg
     1561 attcaccgcc cggcgttctt cttctgtcaa tactacccgc aaccgcaggc acgccgggcc
     1621 gccgccattc gccatgcttt cacgtaaatc aaagactttt agttcgctaa tcgggttgtc
     1681 agcggcaagg agttcattga gataacccca tactccggcg tgttcccgac actcctgagg
     1741 cagcaccaac atcatggaac catcatcgcg gctcaacagt tggctgttaa acagataggt
     1801 agacaccgca tccgacacgg aaacctgagt tgccggaact tctatcgcca taaaaccgtt
     1861 cacccgcgcc cgcaggtttg ccagtaactg tgactggcga gcgaacgcct gttggtggca
     1921 aaacagcacc tggcggttac ttacggcaat cacgtcatta tgaaaaacac cctggtcgat
     1981 aacgtccggg ttttgctggg cgaaaatcac ctgttgggga ttcacctgat taagccttgc
     2041 caccgcctcg ctggcttcgc gagtctgtcg cgccggataa cgggaaggcc gggtatcatt
     2101 gccttcttct cgcccgtaga caaaaagttg catacccggc tcaccgtaat gaccgccgag
     2161 acgattgtgg tttgccgccc cctcatcacc gagcaacgct acctgcggca acgccgaatg
     2221 gacgctaaat ttctcttcgt cgttaaaaat agcttttaac agcgattcag tgacgggcgc
     2281 ttccagcgaa cggtgaaatt tattgttcag gttggcaacg gtgagatgca ctttaccatc
     2341 cagcgtatcg gcagatggcg cgatcgttgc cgcattggct acccacattg gcgaagcgga
     2401 gctgacgctg gaaagccagt gcggtgcctg gcgtgcaact ttttccagta cctgctcatc
     2461 gctaccactg aatcccaact gacgcagcac cggaataaac ggacgctcgt gcggcgggat
     2521 cacggcctgg gggaatcccg catcggcaag ggttttcatt ttcagtaagc cctgtttcgc
     2581 cgccagtcgc gggttagaca cctgaaaacg gtgacgggta gaggcttcat taccaaacga
     2641 caggccagcg taatgatgcg tcagccctac cagcccgtcg aaattaactt cccaggcgtt
     2701 catcgcacca cctcatcgga aaaatccagc ccggggttaa gcgttgcggg caaagttaac
     2761 gagtccgact ccaggctcgc cataggccat gcgcagtaat ctgcggcata ccaggcgctg
     2821 gggcgatggt taccggatgc accaatgccg ccgaatggcg cggtactggc agcaccagta
     2881 agcggtttgt tccagttaac aatccccacc cgcgcctcca gcaacagttg atcgaatttt
     2941 tcccgctcgg gggaaaccag accgcaagag agtccgaagc gagtgttatt cgccattaga
     3001 atcgcttcct cgaaagaatc ataacgccag acgcgcaata acggtccgaa cacctcttca
     3061 tctggtacgc cagcaacgcc tgtcatttca atgatccccg gcgtcagcaa cgatgtctct
     3121 gattgtaata agcgcggcgc aagcagggtt cgtccgccca tcgcttccag ttgctgctag
     3181 gcagtaacca cctgctgtgc ggcctgttca gaaatcagcc cgccaataaa cggctgcggt
     3241 tcgtcatccc agttgcccgg cgttaatcgc tggctgacgg caaccagacg agcaagaaac
     3301 gcatcgccct gtgctccgct tttgagcaat aaacggcggg cgcaggtgca gcgttgcccg
     3361 gctgtgacaa acgccgactg aatggtcaga tggacagccg agtcgatatc cgccacctca
     3421 tcgataatta gcggattatt accgcccatc tcaagggcga gaattttctc cggctgaccg
     3481 gagagctggc gatgcagctg gtagccggta ttggcgctac cggtaaacag caaaccgtcg
     3541 agatcctcca gcgcactcag cgcctgaccc gtttcacgcc cgccctgcac caggttcagc
     3601 acgcccggcg gcaagccagc ctgctgccat aaacgcatta ccgcttcgcc actccacggt
     3661 gtcagttcgc tgggtttaaa gataatggtg ttacctgcca gcaatgccgg aacgatatgt
     3721 ccgttcggca aatgaccagg gaaattatac ggcccaaaca ccgccagcac gccgtgcggg
     3781 cgatgtcgca ggctcgccgc accgtccggc atttcactac gctgctcgcc ggtacgaacg
     3841 tgatacgcct taattgatat cgcgatttta ttgatcatcg ccgtcacttc ggttgccgct
     3901 tcccagcgcg gcttacccgt ttctctggca ataatcgcgg ttaattcggc tttattgctt
     3961 tccagcaatc cggcaaagcg ttcgacaacg acctgacgtt cagccaatga gagccgcgcc
     4021 cagcgcggaa acgccgcacg ggctgcccga caagcctgtt cgacctgagc ggcatcggca
     4081 tcattgcctt gccataacac ctcgcccgat accggattac gcttcacacg cgatgcgccc
     4141 tggcccgtta tccagtcacc gttaatccat aaagtcatgc tgttttctcc tctgcgcaca
     4201 ggcgcaccag acgaacgcga tcccctgcgt ggcatttgag ggcatccagt tgtgcggcgg
     4261 ttaaaatcaa acgctcggtt gccggatcgg tacgcgccag caccacgcgg aaatggtgat
     4321 aattttcatt ggcgaccagg caggccggaa aatcgccctg cgcaggctgc ccttctgcca
     4381 cttccaccag ccgactttta cggatggcgc gcacgcggtc gatgtcacac tcaagcgttg
     4441 gcccgccgtc gaagatgtcg atatagttgc ggtaacgaaa accttctttc tccagcaccg
     4501 cgcgggcagg cgcggtttgc ggatgtacct gaccgatgac gtcctgggct tcctgggata
     4561 aaaagtgggt atagatcgga tgtttcggca tcagttctgc aataaatgcc ttttgcccgg
     4621 tgccgcagag aaaatcgacg cggctaaaat ccatcgaaaa gaagcgttta ccgaggcttt
     4681 gccagaacgg tgaatagccg tgttcgtcaa tcaccccgcg catttcagca accactttgt
     4741 cattaaactt gtcgcgaaaa gccgccataa acataaagcg cgatttcgac agcaaatagc
     4801 cgttgccctc tttgcgccag tccgggtcga gaaacagcgt gcacagctcg ctgctgccgg
     4861 tgtgatcgtt actaagaaac agcgtcggca atgcgttata gacattcagc tcttttgagg
     4921 cgtgaaccaa cgtgccgacc cgatagttgt accagggatc gttcagccca accgccacct
     4981 caatggcgca aatccccgcc acggtgcctg tctcgctatc ttccagcacg aacacatagc
     5041 cctgctcgct tttgggcagt tcgccttgcc aggttttgat tgccctttcg atacgcgcgg
     5101 aaagcgtggc ttcattggcg ggaagcgacg tcaggccgcc gcccgttttg ctggcaagct
     5161 gcatcagcgc cgagacatct gagcgctcaa cgggacggat gaccatcatg atgaacctcg
     5221 gctaacaaag tgttcgcaag cagctgcaaa gcgatccagt ccggtcgtca cctcttcttc
     5281 gctgacattg agcgcaggcg caaaacgcac cacgttgcca cccgcaatca gtaccataac
     5341 gcctgctttc gccgcctcct gagagatctg tttcgcttgt ccggcgtaat cggcattcag
     5401 tacacagcca atcaacaaac ctaagccgcg aacttcactg aacaaaccgt agcggtgatt
     5461 aatggtatta agacgctcaa caaaccagtc gtgacgctgt ttaacgccat taagcatctc
     5521 tggtgtgttg atgagctcca gcactttgcc cgccaccgcc gaggccagcg gattgccgcc
     5581 ataggtggtg ccatgagtgc caacggtcat cacgctggcg cactcttcgg ttgttaacaa
     5641 cgcaccgacc gggaaaccgc cgcccagcgc tttggcggta gttaacagat caggtgtcac
     5701 gccgtagtgc atataggcat acagttcccc ggtgcgcccg acgccggttt gtacttcatc
     5761 aaaaatcaac agcgcattgt ggcggtcaca caattcacgc agaccttgta aaaacgcgtt
     5821 gctggctggc accacaccgc cttccccctg gatgggttcg acaatcaccg cacaggtggc
     5881 gtcgtcaatc agcgcgctgg cagagttaat atcgttatat gcagcatgac ggatatctgg
     5941 tggcagtggc gcaaaatcct gtgaataggc tggctgccca cccgcactga cagtaaacag
     6001 cgtgcgacca tgaaacgcat ttttaaacgc cacgataccg ctcttatggc tgccgtagcg
     6061 gtcgtgagcg aatttacgcg ccagttttag cgccgcttcg ttggcttccg caccggagtt
     6121 acaaaagaag acgcgatcgg caaacgtggc gtcgatcaat tttttcgcca gtcgcagtac
     6181 cggctcgttg gtgtaaccgt tgccggtatg ccagaactta ctcgcctgtt cgttcagcgc
     6241 ttcacgcagt tccggatgcg catggcccag cgcgttcacc gcaatgccac ccgcgaagtc
     6301 gatatactct ttcccctgct gatcccacaa gcgcgaacct tcgccacgta ccggtataaa
     6361 gggtgccgga gcgtaaacag gtatcatcca ttcatcaaag ttttcacgcg taattggctg
     6421 agacatagcg acctctacag taaataattc gctatctata tgttaacaat aagtaatgtt
     6481 tgcgctgtaa atgtagattg cagggttcgt gccagccagt gataaaagtg cataaacggc
     6541 ggaggctaac tggaaatcaa ggagttataa ccaaaccata tgcatttaaa gtgcatataa
     6601 agtgaatacg tttgcgatgt gggtgaataa aaagaataaa aaacgcaatg ttatgcagaa
     6661 gtaaaatata attctggaat tgtgatcatt gacgaaattt actggaaatt actgcgccat
     6721 tctgacgcag cgcgcaccaa aagcgggcat tttttgcgcc atcgttgaca tcattaacaa
     6781 ccatcgatca aatcacttaa caacaggcgg taagcaacgc gaaattctgc taccatccac
     6841 gcactcttta tctgaataaa tggcagcgac tatgaaattt gtctctttta atatcaacgg
     6901 cctgcgcgcc agacctcacc agcttgaagc catcgtcgaa aagcaccaac cggatgtgat
     6961 tggcctgcag gagacaaaag ttcatgacga tatgtttccg ctcgaagagg tggcgaagct
     7021 cggctacaac gtgttttatc acgggcagaa aggccattat ggcgtggcgc tgctgaccaa
     7081 agagacgccg attgccgtgc gtcgcggctt tcccggtgac gacgaagagg cgcagcggcg
     7141 gattattatg gcggaaatcc cctcactgct gggtaatgtc accgtgatca acggttactt
     7201 cccgcagggt gaaagccgcg accatccgat aaaattcccg gcaaaagcgc agttttatca
     7261 gaatctgcaa aactacctgg aaaccgaact caaacgtgat aatccggtac tgattatggg
     7321 cgatatgaat atcagcccta cagatctgga tatcggcatt ggcgaagaaa accgtaagcg
     7381 ctggctgcgt accggtaaat gctctttcct gccggaagag cgcgaatgga tggacaggct
     7441 gatgagctgg gggttggtcg ataccttccg ccatgctaat ccgcaaacag cagatcgttt
     7501 ctcatggttt gattaccgct caaaaggttt tgacgataac cgtggtctgc gcatcgacct
     7561 gctgctcgcc agccaaccgc tggcagaatg ttgcgtagaa accggcatcg actatgaaat
     7621 tcgcagcatg gaaaaaccgt ccgatcacgc ccccgtctgg gcgaccttcc gccgctaatt
     7681 tagcagctct cctggctcaa actgggtcag gagaattaac cttgagaaaa atcaacaaac
     7741 tgtcagtaat gatttgttgc ctgccgtcct ttgttatacc gtcactgcgt ttttagttgt
     7801 ctgaccactt ctctattatc aagtttgata taggaaactc cacgatgaat gctgagcgta
     7861 aatttctttt cgcctgtctt atttttgcgc tggtcattta cgctatccac gctttcggtt
     7921 tattcgatct gctcaccgat ttaccccact tacagacact cattcgccag agcggattgt
     7981 tcggttatag cctctatatt ctgttattca tcattgccac cctctttgtg ttaccaggaa
     8041 gcatactggt gatcgctgga ggaatagttt ttggcccgct cttagggaca ctactctcat
     8101 taattgccgc cacgctggcc tcctcgtgct cattcctgct ggcgcggtgg ctgggacgtg
     8161 atttactgct gaaatacgtg ggccatagcc ataccttcca ggccattgaa aaaggcattg
     8221 cgcgtaacgg tattgatttt cttattctga cccgcttaat cccgttgttt ccttacaata
     8281 ttcaaaatta cgcttacgga ttaaccacca tcgccttctg gccttatacc cttatttcgg
     8341 cactcacgac cctgcccggt attgttattt ataccgtgat ggcaagcgat ctcgccaatg
     8401 aaggcattac gctgcgcttt attttacaac tctgtctggc gggcctggcg ctgtttattc
     8461 tcgtccagct cgcaaaactc tacgcccgac acaaacatgt ggatctgtct gcttcgcgcc
     8521 gcagcccgct tactcaccca aaaaatgaag gatagaacga tgtcgcaaca ttattcagtg
     8581 tcatggaaaa aaggactggc tgcactctgc ttactggctg ttgccgggct tagcggctgc
     8641 gatcaaaaag agaatgcggc ggcaaaagtg gaatacgacg gactttcgaa cagccaaccg
     8701 ctgcgcgtcg atgccaataa ccatacggta accatgttgg tgcaaattaa tggtcgtttc
     8761 ctcaccgacg acactcgtca cggtattgtg tttaaagatg gctccaacgg acataaatcg
     8821 ctgtttatgg cttatgcgac cccgaaagca ttttatgaag ccctgaaaga ggcaggtggt
     8881 acgccgggcg aaaacatgac gatggataat aaagaaacga ctcatgtcac aggcagcaaa
     8941 ctggatattt cggtcaactg gcaaggggcg gcaaaagcgt attccttcga tgaagtgatt
     9001 gttgacagta atggcaagaa actggacatg cgctttggcg gtaatttaac ggcagcagaa
     9061 gagaagaaaa cgggttgcct ggtgtgtctg gatagctgcc cggtcggcat cgtcagcaat
     9121 gcaacataca cttatggtgc ggtggaaaaa cgcggcgaag tgaaattcaa aggcaatgcc
     9181 tcagttctcc cggcggataa cacgctggca acggttacct ttaaaatcac cgaataaagc
     9241 caggataaag gatgatgaag atgcaatcgc gtaaaatctg gtactaccgt ataaccctca
     9301 tcatcctgtt gttcgctatg ctgctcgcat gggcgctgct tcccggcgtc catgagttta
     9361 tcaatcgcag cgttgcggcg tttgccgccg tggaccaaca gggtatagaa cgctttattc
     9421 agtcttacgg cgcactggcg gcagttgtct cgttcttgtt gatgatttta caggccattg
     9481 ctgcaccgct acctgcgttt ttgatcacct ttgccaatgc gtcgctgttt ggcgcgttct
     9541 ggggcggctt gctgtcgtgg accagttcga tggccggcgc ggcgctgtgc ttttttatcg
     9601 ccagagtgat gggccgcgaa gtggtggaaa aattaaccgg caaaaccgtg cttgacagta
     9661 tggacggctt tttcactcgc tacggcaaac acaccatcct ggtctgtcgg ttattgcctt
     9721 ttgtcccttt cgatccaatc agctatgctg ccgatctgac ttcaatacgt tttcgctcgt
     9781 tttttatcgc caccgggctt ggtcaattac cgacgactat tgtttattcc tgggcgggca
     9841 gcatgttaac aggcggtact ttctggtttg tcaccggact gtttattctg tttgccctga
     9901 ccgtggtgat ttttatggcg aagaaaatat ggcttgagcg ccagaagagg aatgactgat
     9961 gggtttaccg ccgcttagca aaattccttt cattttacgt ccgcaggcgt ggctgcatcg
    10021 tcgccattac ggcgaggtgc taagcccgat tcgctggtgg gggcggatcc cgtttatctt
    10081 ttatttggtg tcaatgtttg ttgggtggct ggagcgcaaa cgctcaccgc tcgatccggt
    10141 agtgcgatcg cttgtcagcg cgcgcattgc gcaaatgtgc ctgtgtgagt tttgtgtgga
    10201 tatcaccagt atgaaagtcg ccgagcgtac cggcagcagc gataaactgc tggcagtggc
    10261 tgactggcgg caaaacccgc tctttagcga tgaagaacgg ctggcgctgg agtacgccga
    10321 agccgcaagc gtcacaccgc caacagtcga tgatgccctg cgtacccgac tggctgcgca
    10381 ttttgacgct caggcgctca ccgaactgac ggcattgatc ggcctgcaaa acctgtcggc
    10441 tcgttttaat tctgccatgg acattcccgc tcaggggctg tgccgtattc cggaaaaacg
    10501 ttcttaagga gagaagatgc gccattgtgg gtggttgctg ggattgttat cgctgttttc
    10561 tttggcaaca catgccagtg actggcagga aattaaaaat gaggccaaag ggcaaaccgt
    10621 ctggtttaac gcctggggcg gcgataccgc aattaaccgc tatctcgact gggttagcgg
    10681 cgagatgaaa acccattacg ctataaacct gaagattgtt cgtctcgcgg atgccgcaga
    10741 cgcggtgaag cgcattcaga ccgaagctgc tgccggtcgt aaaacgggcg gctcggtgga
    10801 tctgctctgg gtgaacggcg aaaacttccg caccttaaaa gaggccaatt tattacaaac
    10861 gggctgggcg gagactctgc ccaactggcg ctatgtcgac acacagctgc cggtgcggga
    10921 agatttttca gtgccgacac aaggtgcgga atcgccctgg ggcggcgcac aactgacgtt
    10981 tatcgcccgc cgcgatgtta cgccacagcc accacaaacg ccgcaagcct tactggagtt
    11041 tgctaaagcc aatcccggca cggttactta tccgcgccca ccggacttta ccggcacggc
    11101 gtttcttgaa cagttgctga ttatgctgac gccagatccc gccgcattaa aagaagcgcc
    11161 ggacgatgcg actttcgccc gtgtcactgc gcccttgtgg caatatcttg atgcactgca
    11221 tccgtatttg tggcgcgaag gaaaggattt cccgccttcg cccgcgcgga tggatgctct
    11281 gctgaaagcc ggcacattgc gcctgtcgct gacctttaac cccgcgcatg cgcagcaaaa
    11341 aatcgccagc ggcgatttgc cggcaagcag ttacagtttt ggctttcgcg aggggatgat
    11401 tggcaacgtg catttcgtca ccattccagc caacgcgaat gccagtgctg cggcgaaggt
    11461 agttgccaat ttcctgctct cacccgatgc gcaactgcgt aaagcagatc ccgttgtctg
    11521 gggcgatcct tctgttctcg atccgcaaaa actgcctgac gggcagcgcg aaatattgca
    11581 atcaagaatg ccgcaggatc tgccgccggt actggctgaa ccgcacgcag gatgggtgaa
    11641 tgcactggaa caagaatggt tacgccgtta cggtacgcat taatcttttt gctgtgggcg
    11701 atggtggcgg tgatttatgc accgctgatc ccggcagctc tcacgctgat atcgcctgcg
    11761 ttgtcgttga cacactggca ggcgttattt gccgatccac agttaccgca tgcattactg
    11821 gcaacgctgg tatcgacaac catcgcggcg gtcggggcat tgttgattgc cctgctggtg
    11881 attgtggcgc tatagcctgg gccgaaatgg cagcgtatgt gcgcccgtct gccgtggctg
    11941 ctcgccattc cccatgtggc ttttgccacc agcgccctgc tgctctttgc tgacggaggg
    12001 ctgctatatg actatttccc gtatttcact ccgccaatgg accgatttgg catcggtctg
    12061 ggcctcaccc ttgcagtgaa agaaagcgca tttctgctgt ggatcttagc ggcagtattg
    12121 agcgaaaaac ggctgttgca gcaggtcatt gtgctggatt cgctgggcta cagccgctgg
    12181 caatgcctga actggctgct gttgccctcc gtcgcgcctt cgctggcaat ggcgatgctg
    12241 gcgattgttg cctggtcgct ttcggtcgtg gatgtggcga ttattcttgg gccaggtaat
    12301 ccaccgacgc tggcagtaat tagctggcag tggttaactc agggcgacgc ggatcaacaa
    12361 acgaaaggcg cactcgccag tctgctgctg atgctgttac tcgccgccta cgttttgctg
    12421 agctatctgc tatggcgcag ctggcggcgc actattcccc gcgtagatgg cattcgcaat
    12481 gcctgccaca cctttattac caggcactac gctggcgagt tttttaccct taaccggtgt
    12541 gctgtgtgtg gttctgctgg cgatcctcgc ggatcagtcg acgatcaata gtgaagcact
    12601 catcaacagt ctgacaatgg ggctggtagc aacattcatc gctttgctcc tgttactgct
    12661 gtggctggaa tgggggccac agcgtcgcca gttgtggcta tggttgccca ttctattgcc
    12721 cgatctgcca ctggtggcgg gccagtacac gctggcgcta tggctgaacc tggatggaag
    12781 ctggacggcg gtggtctggg ggcatctgct gtgggtaatg ccgtggatgc tgtttatcct
    12841 gcaacccgcc tggcagcgca ttgattcacg gttaattttg attgcgcaaa cactgggctg
    12901 gtcgcgggcc aaaatcttct tttacgtgaa atgcccactc atgttgcgcc ctgtgctgat
    12961 tgccttcgcg gtgggatttt cagtcagtat tgcgcagtat atgccgacgc tgtggctggg
    13021 cgcggggcgt tttccgacgc tcaccactga ggcggtggca ttaagcagcg gcggcagcaa
    13081 cggtattctc gccgcccagg ctttatggca actgttgtta ccgcttatta tttttgccct
    13141 gaccgcgtta gtcgcaaaat gggtaggtta tgtcagacaa ggactccgct aatgctctgc
    13201 gtgaaaaatg tttcgctacg tttaccagaa agccgcttgc tgacaaacgt taactttacg
    13261 gttgataaag gtgacatcgt cacgttaatg gggccgtctg gctgtggaaa atccactctg
    13321 ttttcatgga tgattggtgc actggctgga cagttttctt gtacaggtga gctatggctc
    13381 aatgagcaac ggattgacat gctacccacc gcacagcgtc agattggcat tcttttccag
    13441 gatgcactgt tatttgacca gttcagcgtc gggcaaaatt tactgctggc gctactgtcg
    13501 acgctcaagg ggactgccag gcgcaatgcc gtgaaagacg cgcttgatcg agcgggactg
    13561 gctgaaactt atcaccagga ccctgcgaca ttatcaggcg gtcagcgagc gcgggtggcc
    13621 ctgctgcgcg cccttctcgc tcagccaaaa gcgttactcc tggatgagcc attcagccgt
    13681 cttgatgtgg ctctgcgcga taattttcgc cagtgggtgt tcagcgaagt tcgcgaactg
    13741 gcgatccccg tcgttcaggt aacgcacgat ctccaggatg ttcctgctga tagttctgtt
    13801 ctggatatgg cgcagtggtc agaaaattac aacaaactgc gataacgcaa agtttttctc
    13861 aatgcgtcag ttcagaatgg cgcactcaaa actacaatgt cgggattttc gatgaaacgt
    13921 gtttctcaaa tgaccgcgct ggcaatggct ttagggctgg cttgcgcttc ttcgtgggcc
    13981 gctgaactgg cgaagcctct tacacttgac cagcttcaac aacaaaatgg caaagcgata
    14041 gatactcgcc ccagcgcgtt ttataacggc tggccacaaa ccttaaatgg cccttctggt
    14101 catgaacctg ccgccttaaa cctctctgcc agctggctcg acaaaatgag caccgaacag
    14161 ctcaacgcgt ggatcaagca acataacctg aaagccgatg ccccggtggc gctgtacggt
    14221 aatgacaaag atgtcgacgc cgtcaaaacg cgactgcaaa aagcaggttt tacgcatatc
    14281 tccattctga gtgacgcgct aagcgaacct tcccgtctac aaaaactgcc gcactttgag
    14341 cagctggttt atccgcaatg gctgcacgac ctgcaacaag gtaaagaggt tacggcgaaa
    14401 cctgccggtg actggaaagt cattgaagcg gcctggggcg ctcctaagct ttaccttatc
    14461 agccatattc ccggtgctga ctacatcgat accaacgaag tggaaagcga accgctgtgg
    14521 aacaaagttt ctgatgaaca actaaaagcg atgctggcaa aacacggcat tcgccatgac
    14581 accacggtca ttctgtacgg gcgtgacgta tacgctggag cgcgtgtggc gcaaattatg
    14641 ctctacgcag gcgtgaaaga tgtgcgtctg ctggatggcg gctggcaaac ctggtccgac
    14701 gcgggactgc ctgttgagcg aggaacgcca ccgaaagtga aagcggaacc ggatttcggc
    14761 gtgaagctcc cggcacaacc gcagttgatg ctcgatatgg aacaggcccg tggactgctg
    14821 catcgccagg atgcatcgct ggtgagcatt cgttcgtggc cagaatttat cggtacgacc
    14881 agcggttaca gctatattaa accaaaaggt gaaatagccg gagcacgttg gggacacgct
    14941 ggtagcgact cgacgcatat ggaagatttt cataacccgg atggcaccat gcgcagcgcc
    15001 gatgatatta ccgctatgtg gaaagcgtgg aatatcaaac cagagcagca agtttcattc
    15061 tactgcggca ccggctggcg cgcgtccgaa acctttatgt acgcacgcgc catgggttgg
    15121 aagaatgttt ccgtgtatga cggcggctgg tacgaatgga gcagcgatcc aaaaaatccg
    15181 gtagcaaccg gtgaacgcgg cccggacagt agcaaataac attgcaatta ctgacgctgg
    15241 agcgacttca gcgtcagata cccactccac acccgcgtaa aggtcgtcat ccagcacaac
    15301 gcaccgaaga tccacgcaaa ccacgcaaac catgcaggga ataaacatcc cagcacaaac
    15361 agtaagatcg tttctgtgcc ttcggttaat ccccccagat agtaaaacga cttatgtgca
    15421 taaccggggt tatcaatctg atgttttgct gccagtgcag caaaggcgag aaaactgctg
    15481 cccgtgccga taaacgcaaa caacaaccag ccgcctgcca gcgcattttg ctccggtgca
    15541 gcaagaataa agccaaacgg caccagcgcg taaaaga
//
LOCUS       DAJVRF010000093        15491 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJVRF010000093 DAJVRF010000000
VERSION     DAJVRF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.093"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..843)
                     /gene="adrA"
                     /locus_tag="OQN13_002699"
                     /gene_synonym="dgcC"
     CDS             complement(<1..843)
                     /gene="adrA"
                     /locus_tag="OQN13_002699"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCS1616011.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(945..1265)
                     /gene="psiF"
                     /locus_tag="OQN13_002700"
     CDS             complement(945..1265)
                     /gene="psiF"
                     /locus_tag="OQN13_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCS1616012.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1384..2799)
                     /gene="phoA"
                     /locus_tag="OQN13_002701"
     CDS             complement(1384..2799)
                     /gene="phoA"
                     /locus_tag="OQN13_002701"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCS1616013.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2900..3160)
                     /gene="iraP"
                     /locus_tag="OQN13_002702"
     CDS             complement(2900..3160)
                     /gene="iraP"
                     /locus_tag="OQN13_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCS1616014.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(3342..3545)
                     /locus_tag="OQN13_002703"
     CDS             complement(3342..3545)
                     /locus_tag="OQN13_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616015.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            3623..4717
                     /gene="ddlA"
                     /locus_tag="OQN13_002704"
     CDS             3623..4717
                     /gene="ddlA"
                     /locus_tag="OQN13_002704"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS1616016.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALKRHAADNALKATM"
     gene            complement(4741..4953)
                     /locus_tag="OQN13_002705"
     CDS             complement(4741..4953)
                     /locus_tag="OQN13_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCS1616017.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            5213..5521
                     /locus_tag="OQN13_002706"
     CDS             5213..5521
                     /locus_tag="OQN13_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCS1616018.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(5580..6674)
                     /gene="yaiW"
                     /locus_tag="OQN13_002707"
     CDS             complement(5580..6674)
                     /gene="yaiW"
                     /locus_tag="OQN13_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCS1616019.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(6687..7907)
                     /gene="sbmA"
                     /locus_tag="OQN13_002708"
     CDS             complement(6687..7907)
                     /gene="sbmA"
                     /locus_tag="OQN13_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCS1616020.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            8259..9416
                     /gene="ampH"
                     /locus_tag="OQN13_002709"
     CDS             8259..9416
                     /gene="ampH"
                     /locus_tag="OQN13_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCS1616021.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(9417..10040)
                     /gene="iprA"
                     /locus_tag="OQN13_002710"
     CDS             complement(9417..10040)
                     /gene="iprA"
                     /locus_tag="OQN13_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCS1616022.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(10128..13010)
                     /locus_tag="OQN13_002711"
     CDS             complement(10128..13010)
                     /locus_tag="OQN13_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1616023.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITNGGVLDVNTNMNDISSVFAT
                     DTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMNSD
                     GHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYIDD
                     TKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQ
                     DTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGNNA
                     KWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSA
                     TMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSNDMK
                     VDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTE
                     GSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            13536..14510
                     /gene="hemB"
                     /locus_tag="OQN13_002712"
     CDS             13536..14510
                     /gene="hemB"
                     /locus_tag="OQN13_002712"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCS1616024.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(14616..15467)
                     /gene="tauD"
                     /locus_tag="OQN13_002713"
     CDS             complement(14616..15467)
                     /gene="tauD"
                     /locus_tag="OQN13_002713"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCS1616025.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
BASE COUNT         3924 a         3906 c         3712 g         3949 t
ORIGIN      
        1 accgcgcagg gtaatttgta actgtcgggt aagcgccaca atcgcttcat cgcccacatc
       61 atgaccccag gtatcgttga tgctcttgaa atggtcgata tcgataatca gtaacgttgc
      121 atcgcgatta tgccgccgac agttatcaaa ttcattgcgt aacatagttt cccaatgacg
      181 tcggttatac acgcccgtca tgccgtcgcg agtactcatg acctgcaacc tgcgtttatg
      241 ttccgccagt ttggttgccg tctggtagct gacccagcca aacagcagag gataaacgac
      301 aataatggga agggagagcc accattccag cggcgcacta ttgaacgaca cggtaatgcc
      361 cgtaagctcg agggtgacaa ggcaggaaac caccatcaac accagacccg cgacaaacag
      421 acgggggccg cctgccccca tcaaattcag acacataatc atcaacatag cggtggaagg
      481 cagcacgttt acgcccatta cgcctaccca cattcccgct aatactgcat cggtttttaa
      541 gttgtaaatt tcccggctaa gcggatcgac ggccctgctc gctatctgcc aggctaaatg
      601 cggccagacg aacgcccagc cgaccaacac cagccaccac cagcccggcg gcgggtgcga
      661 aaccagcgtt gaagcaatcg gtaagaacat gccagccagg ccaaccgcac ggggtagtct
      721 gacgcgacgg gcgaagcgca gcccggaccg ctgatgttcg ttttgaggag ttaaaggagg
      781 ttcctcccca tgcgccgccg cttttttgaa aaagttttca tcattcatta tttttgggaa
      841 cattcagaaa caattttccc aaattataga gacggggcta aaggcagaag tatgaaattt
      901 cgggtgagcc actttcgtag ctcacccgtt agcactatca ggtatcaggc ggctttcttg
      961 aggcaggcac tcataaactt attacgatca tcacctttca gagattgttg cgttgcttga
     1021 ttattacatt cgcgcatctt ttgctgctgt ggcgtcaaac ttttttcacc aggcgcagac
     1081 ttgctgttct tcaggcaatc actcatgtag gtcttacgag catccccttt caacgcctgc
     1141 gccgtcgcct gctgattaca ggaggtcata cgctgttgtt gtggggttaa ggttctctcg
     1201 gcagcgccga cggtggttaa aaaaaccaga ccaaaaagca aggtaaccag taatgttatt
     1261 ttcatagcac catccctctt catgttttaa ccatgagcgt ttgcgcccgt gatctgccat
     1321 taagtctggt tgctaacagc aaaaaaacca cccggcagcg aaaattcact gccgggcgcg
     1381 gttttatttc agccccagag cggctttcat ggtgtagaag agatcggtct ggtcggtcag
     1441 tccaacaaca ttggcggcat gcggaccata cgccgcaata cgcaactgac tgccggtatg
     1501 ttcttgtgaa tcctcttcgg aattcccgta actcatcacc atcactgcgc catctttagt
     1561 atttagcgcc tgagtgaggc ccggcgcttt ggtatccggc gcaacaacct ggctggcgtg
     1621 ggcgtgatca gcggtgacta tgaccagcgt gttaccctcc tttttagcga attccagcgc
     1681 ccgttgtacg gcttcatcga gatcgaccgt ctcgccaatt tgcccacaag gattcgcagc
     1741 gtgatcctgt ttatcgattg acgcaccttc aacttgcagg aaaaatcctt tctcattttt
     1801 actcaacaat tcaatggttt tgtcggtcat ctgtgccagg gttggtacac tgtcattacg
     1861 ttgcggatta ggcgtacagg tgactgcggg cttatcgata ttgccgtggt acgttgcttt
     1921 cggccctagc cagcgcactg gcatattgcc gtcagcaaac aggccaagca ggggtttttg
     1981 ctgattcgct tccgtcaccg aattcagtga ggcagcatcg ttcaccaact gataaccacg
     2041 cgcctgtgcc tgttcacgca gcgtttttcc ctgccattca ccagcggttg ccgtttcagc
     2101 aaaggttttt gcgccgccgc caagcgtaac gtcggcacga gcgttaagca gctgttcggt
     2161 aatcgatcct tttccgcctt tttccagagc gttacccgga catttttcac tggtcgcgct
     2221 cggaccgtag catttgcgcg aagtcacatg tgccaccagc gcagcgggcg tggcatcctg
     2281 caactctgcg gtagaaacgt taccggtcgc cagacctgcg gcttttgcca tttccagaat
     2341 cgttgggtga tctttttcgt gaatatcgac gcccagcgcg ccgttatagg ttttgacacc
     2401 ggttgaccag gcggttgctg atgcagccga gtcggtgacg tagtccggtt tgccagtttt
     2461 tttattcagc gcatagtgag tgtattgccc ggtaagcggt aaggcatcga tacctttaaa
     2521 aaagccgccc gcaccttcgg cataattacg tgcggcagta atttccgaat cccccatccc
     2581 atcgccaatc agcaaaataa tattttttgc aggtttatcg ctaagagaat cacgcagagc
     2641 ggcggtctga tcactcgtta agcggcgagc accgccgggt gcagtaatat cgccctgagc
     2701 agcccggttt tccagaacag gcatttctgg tgtccgggct tttgtcacag gggtaaacag
     2761 taacggtaag agtgccagtg caatagtgct ttgtttcact ttattttctc catgtacaaa
     2821 tacattaaaa aataaaaaca aagcgactat aagtctcggc cgtgacaact ttatgacagc
     2881 tgttgaaaag attaactttt tactgacgag gatgcttcaa taacttcttt acgtaatcgc
     2941 gcagcagctc cgtatcgtcg tcaggaatgc tggcatcggg ctttacctcg tacagcgccc
     3001 cctctacctg atcaatcaac cgctgttggt cattttgcgc catattgcga agcattgcag
     3061 tgacgataat ctccaaagct tctacctgcg cacacagttc tttcgactct tcttcttttt
     3121 gggcaagctt aaataacaac tcagcaatga gatttttcat gtctgtattt ccttatccaa
     3181 agtatggaga agttaacatt atgtttcaca acttcatagt gtctggaagt attattttta
     3241 tgacaaagga aatactttgc gcaaatattt tttgtttgat taccagcgaa acgtttctct
     3301 tatttaaata attacccaca caaaatatag gcttttaatt atcaaccttt attataaata
     3361 aaagttatga aatatattct cttcgttaac ttataattaa gagaaaaaaa ctcaccgcat
     3421 tgccatttaa gccaggaata aattagcttc ccgttcatca tttttgtgat ctcgcgcaca
     3481 tctttgagat aacgcacttt cttcaccagt acgttgcacg ctttgtcagc agaagattgc
     3541 aacaatcttt cagaaggccg cgaatccccc gttttgtcgt ggtaatgtat gcactttgtg
     3601 ttagacggga taggttttta agatggaaaa actgcgggta ggaatcgttt ttggtggtaa
     3661 atcagcggaa catgaagtgt ctctgcaatc ggcaaaaaac attgtcgatg ccattgataa
     3721 aagtcgcttc gacgttgtgc tgctgggcat tgataaacaa gggcaatggc acgtcagcga
     3781 tgccagcaat tatctgctaa atgcagacga tcctgcccat attgcgttgc gcccttcggc
     3841 gaccagcctt gcgcaggtgc caggtaaaca tgagcatcag cttatcgacg cgcaaaacgg
     3901 tcagccgttg ccgacggtgg atgtcatttt cccgattgtc cacggtacgc tgggcgaaga
     3961 tggttccttg cagggaatgc tgcgggtcgc caatttaccg tttgtaggtt ctgatgttct
     4021 ggcttcagca gcctgtatgg ataaagatgt caccaaacgt ctgctacgcg atgccgggct
     4081 gaacattgcg ccatttatta ccctgacgcg cgctaatcgt cacaacatca gttttgccga
     4141 agtggagtct aaactggggt taccactgtt tgtaaaaccg gctaatcagg gctcttctgt
     4201 tggtgtcagc aaagtaacca gtgaagaaca gtacgcaatt gccgtcgatc tggcgttcga
     4261 gttcgatcat aaagtgatcg ttgagcaagg gatcaaaggt cgtgagatcg aatgcgcagt
     4321 tctgggcaac gacaatccgc aagccagcac ctgtggcgag atcgtactca ccagcgattt
     4381 ctatgcctac gacaccaagt acattgacga agatggcgcg aaagtggtag ttccggcagc
     4441 cattgcacca gagatcaacg ataagatccg ggcgattgcc gttcaggctt atcagacgtt
     4501 gggatgcgca ggcatggcgc gtgtagacgt gtttttaacc ccagagaacg aagtggtgat
     4561 caacgagatc aacacactgc ctggcttcac caacatcagt atgtatccga agctgtggca
     4621 agccagcggt ctgggttaca ccgatctgat cacacgtttg attgagctgg cgctgaagcg
     4681 tcacgctgcg gataacgcat tgaaagccac aatgtaatat tttaacgccc gataatattc
     4741 ttattgggcg ttttcagttt cttcatcgcg acgacgaata atcaaccccg ccagccagaa
     4801 actaatcacc cacgtcacca gaccgacggc ataggtctgc caaccttttg cttcaaatcc
     4861 cagtaacccc accacgccat taagaatgaa tataaggcca atggcgaacg catagtagtg
     4921 ccagtcacgg cggattttta caggcagatt catggcagct ccgttaacga aaaacaacat
     4981 ccttgcatat tctgtaacaa ccgtctgtgg tgtagacaac attttcgtaa ttgttaataa
     5041 atttaactta aggttgccat tttgtaactc acaacgaaga ttcacgcttc gggaatattc
     5101 ccaacatcaa tttaccatga ttctgatatt gacaatctaa atgacctgtc agacttgcca
     5161 taagccgctg gtatcacacc agaagagaag cagatgactt ccggaggttt ttatggctga
     5221 tttcaccctg tcaaaatcgc tgtttagcgg aaaatatcgc aatgcctctt caacgcctgg
     5281 caacattgcc tatgcgttgt ttgtgctgtt ttgcttttgg gctggggcgc aattgctgaa
     5341 cctgttagtg catgcgcccg gcgtctatga gcgtttaatg caggtccagg aaacaggtcg
     5401 cccacgggtg gaaattggtt taggtgtcgg caccattttc gggctgatcc cgtttttagt
     5461 aggctgcctc atttttgcag tggtggcgct atggctgcac tggcgacatc gccgccagta
     5521 agatttacgt tgccagatac ggcctaaaac gccttatctg gcatgacatt cctgtttgat
     5581 cactgtttca tacaacgcgc ccgccgttcg tctacgcgct tcgcaaacca ggccgtagtc
     5641 aggttgcggg tgattttcgg gctttccagt tgaatgccag gcaacatttc gcggggtagt
     5701 gatttacccg ttttcgcttc ggcaagttga taaactttct tgtacagcgc cgtctcctca
     5761 aaagagaagc tgtcaccttt ctctaactga cggcgaattt cgctgtcgct catccccagt
     5821 tttcccgcca gtttgcgcgt tgccagttcg gttttcccgg gttctttact gtcgtaacga
     5881 attaaatcac cgtccagtgc cagcttcacg ccgctggcct tactgactgc gttctgaaac
     5941 gcggcattac ggctggcgta ccagccagcg ttaaaatcag caaagcggta tatcggtgcg
     6001 ctatagctgg cgggataatt cagtaaatgg taagtaccaa accacaaccc gccgcggcga
     6061 ctgaagactt cctgacgaac tgtaccgtcc attttccacg gatacccttt ggtatgctgt
     6121 tcggcaaaag caatgctgac ctgcatcggc ccaccggtgc gcaccggatt aagcgaacca
     6181 aacagcgtct gccccattgg taccatgctg atcagatcgt caaaaatcgc gctcaattgc
     6241 ttttctgtac gcaccgaatc taaccgttcg ctataacttt tgccgtttgg cgatttgatt
     6301 tttagcgcgg tatgaaccag aaaagcagga atgtgcatcc gttcagcacg acggtcaatc
     6361 tcttgccagg cgatcttgct taagcccgga acggccggat ctgcctgata atttgactcc
     6421 tgctgcgcca ccgccagcac cgagcagacg ttttccagcg tcggtgccag cccctgactt
     6481 tcaaaggtga tggcgagatc tttcgcccag gcatcgcggt ctttcacgct cgcaggcatc
     6541 ttctgccgca cgacgctcgc tacatctatc gctttttcgc cctttttcaa cggttgcgga
     6601 gcctgactac tacaccccgc taaaaccaga accgccaaca atgacagcga agatagatga
     6661 ttgacgcgcg acattacttc tcctttttag ctcaaggtat gggttacttc ctgaattttg
     6721 tcaccatcca gctcatgttc aaagctgcgc agacgtttgt agatagacat caactcaacc
     6781 agtgtggtcc atgagttaat caggtactgg aaagcaccgc gaacctgacc aaaaacgttg
     6841 gtaatctgcg tcatcaggcc gagcgtaatc gtaccggcaa caattgacgg aaacagcaag
     6901 aacaaaccga aaacgttatc gacctgcaaa tagagaatgc gggcgatgtt gaaatacata
     6961 tagtgaaaat agaggcgaaa atagtttttc cgtacggcgc taaacagctc gcgtaccgta
     7021 ggcggcgtcg cgcgcgtggc atcgtcttca ccataaacca gctctttacg gtaggcggcc
     7081 tctactcgct ggtttttaaa ctccagcccc ggcagtttga tccctaccac tgccagccat
     7141 ccggtcccca tcagcgacca gacgatagcg gcaatcacca gaccatacgg aatgtgcccg
     7201 ataatcggca gctccggcac atgcgcggag agcgttacca gcaccggcag gaaggcgatc
     7261 aacgtcatga tggcgttgat aaaactgacg cccatattct ccagcgttga agcaaaacgc
     7321 atggtgtctt cctgcacacg ctgtgcggca ccttcgatat gacgcagttg ttgccagttc
     7381 gccatgtaat attcgttcat cgccgtacgc cagcggaaca catagtgact gacaaagaag
     7441 ttgttcagta cactgatcac caccgcgatc agcgcaatcc ccagaaagac gcccacttcg
     7501 cggtaaaatt gttcgatagt gactttatgc ggcgaactta gcgcggtttg aatcagatca
     7561 tagaacggtg cataccaggc gttgacggcg accccgactt ccaccaaaaa ccaggtgacg
     7621 aagatgatca gtgcagtacc gagtattgac cagtattgcc aacggtgcgg gctgtagata
     7681 aaccagaaaa atgcaaaaag tcctacgcaa acaatgtagt aagcgtaaaa aatcaggaaa
     7741 tccaacgacc agaaacgcgc ggcgctaatc gggatctgcc cggaagcgcc ggtgatacgc
     7801 gccacccagt ctcccccacc ggcttgccag aagataacgg caatcaatgc ccaaacaaag
     7861 gccgagagaa aaaacgctcc cggctttggg aaaaaagact taaacatatg cactcctgct
     7921 aacttcttat cgttttaaca actgctgtgt attaaccgca tgaccactac aaagcgtcag
     7981 ccaaatgaaa ccgcccattg tgacccatag caaggcgctt agttcgccag cctgaccata
     8041 agaattgttt cgacggattt cacaaaccaa agattgttta cataagcgga ttaacacgcg
     8101 caggatcaca aatgcaggca ttggtgagca ggaaaaagat cgtgtttacg cgccgttacg
     8161 tttttaacac tattattgca ctaattcgcc ctttgcaatc catcaatgag tagtataaat
     8221 acgctcagtt accttcattc aatctatgga caccaccgtt gaaacgtagt ctgctttttt
     8281 ctgccgtgct gtgtgcggcg tcattgacct ccgtccatgc ggcgcagccg atcactgaac
     8341 cggagtttgc ctctgatatt gtcgatcgtt atgccgatca tattttttac ggcagcggtg
     8401 caacggggat ggcgctggtt gttatcgacg gtaaccagcg cgtctttcgt agttatggcg
     8461 aaacgcgacc tggtaataac gttcgcccgc agctggattc ggtcgtgcgt attgcttccc
     8521 tcaccaaact aatgaccagt gaaatgctgg tgaaattgct cgaccagggg accgtaaagt
     8581 taaacgatcc gctaagtaaa tatgctccgc caggcgcacg cgtgccgacc tacaatggga
     8641 cgcccattac cctagtgaat ctggcaaccc ataccagcgc cctgccccgt gaacagcccg
     8701 gtggcgcggc acatcgtccg gtgtttgtct ggccaacgcg cgagcaacgc tggaaatacc
     8761 tttctacggc gaagctgaaa gccgcgccag gcagtcaggc ggcgtactct aaccttgcgt
     8821 tcgacctgct ggcagatgca ctggcgaatg cgtccggcaa gccttatacc cagttgtttg
     8881 aagagcagat tacccgtcca ttggggatga aagacaccac ctacaccccc tcaccggatc
     8941 agtgccgccg tttaatggtt gctgagcgcg gtgccagtcc gtgcaataac acgctggcgg
     9001 caattggcag cggcggcgtc tattccacgc ctggcgatat gatgcgctgg atgcagcagt
     9061 atttgtcatc tgatttttat cagcgcagta accaggcaga tcgcatgcag acgttgattt
     9121 atcagcgcgc gcagtttacg aaagtgattg gcatggatgt ccccggcaaa gcggatgcgc
     9181 ttggtttagg ctgggtatat atggccccga aagagggtcg tccggggatt attcagaaga
     9241 caggcggtgg tggcggtttc attacctata tggcgatgat cccgcagaag aatattggcg
     9301 cgtttgtggt ggtcactcgt tcgccgttga cgcgctttaa aaatatgagc gatggcatca
     9361 atgatctggt gaccgagcta agcgggaata aaccgttggt tatccccgca tcctgattaa
     9421 tactctgaag gcaaacggtt gatagcgacc agtttgcctt tattcatttc gatatagccg
     9481 cctttacgca aagcagcgag aacttctgcg acgacagaac gcgatatgcg tgtacgttga
     9541 tggatataat tcatcacacc aatacgtgat cgcaattctt cattccagtc aatcattgat
     9601 aataatgttg cgcggatttg ttcgtaggaa ttatgcccaa tgagctgcac gtcgcgtaat
     9661 tccagaattc tattttgcca agctaaccag taaaaagcgt ctcgccagag ttgattttgt
     9721 tcaataagcg taatggtttg tttggctggt agatgatatc ccgtacaatt tccttctgat
     9781 attaatttgt atggtatatc gtttttcatt aaaccatcag ccagccccat aatataaggg
     9841 gcctgggtaa taccgataag tacgttttct tctctacgca gagagataac tccctctaaa
     9901 ataacgaaag tatcttcgtt attgacatca ctggaaaata taacttgctt ttcattatta
     9961 aactcgaagc gcgtaccgta tctggacaaa catttatcga gcttaccaaa ttcctgaaga
    10021 ggtttaacta cagataacat ttgcgcgtcc tttgcagtaa tgcccgtcaa atccttgacg
    10081 ggcattattt agattaaatt accagtattt cttcggagtg aagaatatta ccaggtatat
    10141 ttaacaccca cgttcgcgga ccagtcttga tctacgtcac caccaccgag gtagttagca
    10201 tcggtatagg cgctgaagtt cttggtgaag ctgaactgag tacccagacc aacacgtacc
    10261 gcagaccctt cagtaccgtt atcgatggaa tcaccgttca catcgttatc gttgttagag
    10321 tcgtcgtaga cgtaagccag tttgaagtac ggagtcagag cctgatcttc gctgtaggtg
    10381 aaggtataac ctgcatctac acccagttca taacgcatgc tgtcgtaaga ctgaccgtca
    10441 actttcatgt cgttgctcag ctggtagtca tcaccagact ggaacagacc agaaacgctg
    10501 ccgtaaggag tcacgtaacc agcatcaccc agtttgaagt cgtaaccggc tttcaagccg
    10561 aagccccaag cgtcggagtt ggtgctaccg tcaacgtaag taccgttgct catggttgca
    10621 gacaggtcgt tgttgaagtg agagtagctc aagctaccat caacaaagac gttgttcgcg
    10681 aagtgagcag aagagtagat gtaggcagtc tggctgtctt gatctacctg accagaacgg
    10741 tcattcatgt cacctttagc gaagcctgca gccgcaccga cgatccactt agcgttgtta
    10801 ccgtcaactt tggtatcaac accgaccatg atgccgttaa catcctgatc atagttgatg
    10861 gtgccgttgt cgccgttgaa gttaccaccg aagtagctta cccatgcgcc gccgttatca
    10921 gccaggccat gacgagagtt ggtcagacga gtaccaacgg tgtcttgttc caggttccag
    10981 atattggtgt tcgcagacgg gatgctcagc gccatgttag cgtagtcggt cagctccatc
    11041 tgttgcagaa caacggtgtt accgcgctgt tcagcctgat aggtgtatgc acccaggtca
    11101 gctttgttag cagcagagaa ggtcgcgttg gttttggtat catcgatata gatgagctgc
    11161 ttacctgcgt aatcagcaat cgcaccagcg ccagtggagt tgtcgatacg aactttgtag
    11221 ttaccggttg cagctacaac gttgtctgca gagctgttgt caacttcgtc gccattgtta
    11281 atgtcgttgt taccattaac ggtcaagtga ccatcagagt tcatcgcaat aacaccataa
    11341 ccgtagttag ctttgctggt gtcgttggta cgatcgttga cgaggtctgc attcaggatg
    11401 taatcattac tatgaatgtc aaacacacct gcgtaaacat taccgttgtt atctttaacg
    11461 ttgccactgg tcagttccag agtatcagtt gcaaatacgc tggagatgtc attcatgttg
    11521 gtgtttacat ccagcacacc accgttagta atggtattgg catacagcgg tgcatatgcc
    11581 caagctgaag cagtatcttc accgaagtta acggttgaga aggtatcaat ggtcaggtga
    11641 tcggtgttaa cttcaccgtt aacttccatt gaagaaccac cgttcagagt gatggtgtca
    11701 gaagtcagag aagaatctgc actcacgtta acctgagaac cgctgttgat cgccagggta
    11761 tcgatcaggg aagttttcgt ggtttcccaa tcagaaccgt tgttcagaac aacatcgaat
    11821 acgccgctct ggaatgcgtt agcgttatca acacgattcc agccatcttc agagtaagaa
    11881 gcgtacgggt tcaggctgtt tggtgcaacg tcgtagaagg cttcgtcagc aacaacaacg
    11941 tcataatcaa tattcgctga accaacccat ttggtgttgt tcagagtcag gttcaggtga
    12001 tcgacgttct gatcgtcagt tgcccaaccg ccatgagtgt aagcagtggt agagttgtca
    12061 atcaggtgac ccgtcgcgtc aaagttagcg ttccaggtac tgtcaaatac aacgtcacca
    12121 agcaactgag agttgttgaa cgtaacgttg ttgaccatac gataatcaga agtgtcatca
    12181 tcgatgaagg ctaacgcaac atcatcagca ggagatgcgc cattgccgta gtcacttggt
    12241 tcaacagaat tgccgaaatg accacgatct gattgttcgt cttcagtcca tgaaccagaa
    12301 gttacagtag agtcattaac aacgatgttg ttgtcccact gatgaccttc ggtattgtag
    12361 gtcgcattac cgaggtcgtg ttgaaccaaa gtaatacctg caacatggga gttgttgctg
    12421 atgttgatgt tactttcaac atccagagtc acagcggtac caagaccagc ggttaccaaa
    12481 cgatcataat ctgtcttaga tgttttaccg tctgcatcca gataagtatc ggtgaagtag
    12541 aaggcgtcaa aatcatcgtc aatagtagaa ttagcgatgt tcagagtgaa gatgtcgcca
    12601 tcaacccagt cgtggccagt ataatcgcta ccgttggtcc agacgtagtc gccataacca
    12661 acctggtaag atgtaaccat accatgaaca attgagttgg agatgttcag tgtattaccg
    12721 gtatctgtgc cataatcgtg gttcagataa taagtagaaa ttacaccatt tacagtgtcg
    12781 ccgctgaaag tggtatcaaa gtaaccatca gctgcgtggt catatacata accaccgtaa
    12841 gtcagataat cgtgatcaat tttagtgctg tcaccccaat tgtagatggt ggctgcgtca
    12901 tcgtgatacc cgaaggtgtt ataagtagct gcattagcct gagaggtgat agccagagtg
    12961 caagccaatg ctaattgtga tactacaagt ttctttttcc aggagtgcat ttgtcatccc
    13021 tcctcaggga cgtaataagt tgatccatca attatcaatg catagaaaac atattatgtt
    13081 tcgcgctaac gattatgctg cgttacagac gaatggttca attattcttt cccctaagtc
    13141 cgaatgtgga catatataat ttatggagaa aaacacgcat caaattgata aaaggacgat
    13201 atttcaacat gttgcagata atgttatttt tgatacatac gcgttataaa tatcaaaaac
    13261 acaaaccaga taaatatctt atcaatatac aacaaagttt gaattattca atttatagca
    13321 gcacaaccaa acgccaaaaa aaataataac caagagctat aaataaagat taaaaatatt
    13381 ttcagtgaat atgaaattat tgctatttct attacaaaga tgtcctggtc tctctttcat
    13441 tctcttcttg tcaacatcac gacaactttc gtaaaacact cctgccccgc ttcaggtata
    13501 ctgtgcacct taattccaca aacatcaggc agaccatgac agacttaatc caacgccctc
    13561 gtcgcctacg caaatctcct gcgctgcgcg ctatgtttga agagacaaca cttagcctta
    13621 acgacctggt gttgccgatc tttgttgaag aagaaattga cgactacaaa gccgttgaag
    13681 ccatgccagg cgtgatgcgc attccagaga aacatctggc acgcgaaatt gaacgcatcg
    13741 ccaacgccgg tatccgttcc gtaatgactt ttggcatctc tcaccatacc gatgaaaccg
    13801 gcagcgacgc ctggcgggaa gatggactgg tggcgcgtat gtcgcgcatc tgcaagcaga
    13861 ctgtgccaga aatgatcgtc atgtcagaca cttgtttctg tgaatacact tctcacggtc
    13921 actgcggtgt gctgtgcgag catggcgtcg acaacgacgc gactctggaa aatttaggca
    13981 agcaagccgt ggttgcagct gctgcaggcg cagacttcat cgccccttcc gccgcgatgg
    14041 acggccaggt acaggcgatt cgtcaggcgc tggacgctgc aggctttaaa gatacggcga
    14101 ttatgtcgta ttcgaccaag ttcgcctctt ccttttatgg cccgttccgt gaagctgccg
    14161 gaagcgcatt aaaaggcgac cgcaaaagct atcagatgaa cccaatgaac cgtcgcgagg
    14221 cgattcgtga atcactgctg gatgaagccc agggcgcaga ctgcctgatg gttaaacctg
    14281 ctggagcgta cctcgacatc gtgcgtgagc tgcgtgaacg tactgaattg ccgattggcg
    14341 cgtatcaggt gagcggtgag tacgcgatga ttaagttcgc cgcgctggcg ggtgctatag
    14401 atgaagagaa agtcgtgctc gaaagcttag gttcaattaa gcgtgcgggt gcggatctga
    14461 ttttcagcta ctttgcgctg gatttggctg agaagaagat tctgcgttaa tttttttcct
    14521 ctcagaaagg gggagttaac ctgttgaaag tacggtgaac gccccctcac cctaaccctc
    14581 tccccaaggg ggcgagggaa ccgtccgcac tcgtatcacc ccgcccgata aaacggttta
    14641 tccccaagga tcgtcgcccg atgcataatc cgccgctgtg gcaggtaatc ggcattggca
    14701 tagtgctgag tcacgcggtt atcccaaatc gcaatatcat tcggttgcca gcgccaacgc
    14761 acctgaaact ccggtttagt gatatgggcg aataaaaagc ctaacaaggc ttcgctctct
    14821 ttctcgctga catcgacaat tcgcgtagta aagccttcat tcacaaacag cgcctgttta
    14881 ccgctcaccg gatgcgttcg caccaccgga tgtagcaacg gaggattttt cgcgaccgcc
    14941 tcgcgccagc gttgatgttc ctcctcggtt ttgcggtatt tgtattccgg gaacgattta
    15001 cggaaatcat gctccgcacg cagcccgctc agcagctggc ggaagggaac agagagcgcc
    15061 tcataggccg caataccgct ggtccagagc gtatcaccgc cggtcgaagg taactcttta
    15121 gctgccagaa tcgcccctgc gggtggcgtt tcaataaatg tcacatcggt atgccagttg
    15181 tcgttatccg gcggattatc gttatgggta tccagcacga tgatctcgtc aaccccttcg
    15241 gcatgagggt aaacagggtg aatatgcagt tcgccaaaac gctgggccag cgcgcgttgc
    15301 tgctgcggcg taatagcttg atcgcgcaga aataccacct gatagcgcag caccgcatgg
    15361 taaagctgtt caaactgatt atcgcttaac gggcgcgtca ggtcggcacc cgaaatttgt
    15421 gcgccgatat acggccccag cggggtaata ctcagacgtt cactcattgt acttctccat
    15481 gccagggcgt c
//
LOCUS       DAJVRF010000094        15381 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJVRF010000094 DAJVRF010000000
VERSION     DAJVRF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15381
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.094"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(44..454)
                     /locus_tag="OQN13_002714"
     CDS             complement(44..454)
                     /locus_tag="OQN13_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCS1616026.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
     gene            complement(512..745)
                     /locus_tag="OQN13_002715"
     CDS             complement(512..745)
                     /locus_tag="OQN13_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HCS1616027.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            1134..1679
                     /locus_tag="OQN13_002716"
     CDS             1134..1679
                     /locus_tag="OQN13_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCS1616028.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1654..3579
                     /locus_tag="OQN13_002717"
     CDS             1654..3579
                     /locus_tag="OQN13_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCS1616029.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQRDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3576..3782
                     /locus_tag="OQN13_002718"
     CDS             3576..3782
                     /locus_tag="OQN13_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HCS1616030.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3779..5380
                     /locus_tag="OQN13_002719"
     CDS             3779..5380
                     /locus_tag="OQN13_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCS1616031.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5361..6680
                     /locus_tag="OQN13_002720"
     CDS             5361..6680
                     /locus_tag="OQN13_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCS1616032.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6690..7022
                     /locus_tag="OQN13_002721"
     CDS             6690..7022
                     /locus_tag="OQN13_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCS1616033.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            7078..8103
                     /locus_tag="OQN13_002722"
     CDS             7078..8103
                     /locus_tag="OQN13_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCS1616034.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            8145..8543
                     /locus_tag="OQN13_002723"
     CDS             8145..8543
                     /locus_tag="OQN13_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCS1616035.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8555..8908
                     /locus_tag="OQN13_002724"
     CDS             8555..8908
                     /locus_tag="OQN13_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HCS1616036.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            8920..9498
                     /locus_tag="OQN13_002725"
     CDS             8920..9498
                     /locus_tag="OQN13_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1616037.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9495..9890
                     /locus_tag="OQN13_002726"
     CDS             9495..9890
                     /locus_tag="OQN13_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1616038.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            9898..10638
                     /locus_tag="OQN13_002727"
     CDS             9898..10638
                     /locus_tag="OQN13_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1616039.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10654..11076
                     /locus_tag="OQN13_002728"
     CDS             10654..11076
                     /locus_tag="OQN13_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCS1616040.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            11058..11492
                     /locus_tag="OQN13_002729"
     CDS             11058..11492
                     /locus_tag="OQN13_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCS1616041.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11485..14046
                     /locus_tag="OQN13_002730"
     CDS             11485..14046
                     /locus_tag="OQN13_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1616042.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSYASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAVKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            14043..14372
                     /locus_tag="OQN13_002731"
     CDS             14043..14372
                     /locus_tag="OQN13_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1616043.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14372..15070
                     /locus_tag="OQN13_002732"
     CDS             14372..15070
                     /locus_tag="OQN13_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCS1616044.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            15076..>15381
                     /locus_tag="OQN13_002733"
     CDS             15076..>15381
                     /locus_tag="OQN13_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HCS1616045.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     "
BASE COUNT         3550 a         3810 c         4742 g         3279 t
ORIGIN      
        1 cagcgagtcc ggttctggca cggaagaaac cggacgttat gatttagtgc ggaaatattt
       61 gtgtagtgtt ctgtatgttc tcagtaaaga gtaatgaatt atcaaaggta tagtaatacc
      121 ttttgttttc gtggatattt gtaatccatc tgaaaacccc tgctgtagca agatttttcc
      181 tgtattcgta aaatgataac tctcctgatt tgaatccttt taaggtggct tctataaggc
      241 atttattttt tgaaaatctt acatttacaa ccttaccctg tccttttatt aaaaccgtat
      301 tatcgttttc aagaacaaga tgaatattct ctgtggctaa atagtaaatg taatgtgaga
      361 cattgtgacg ttttagttca gaataaaacc agtgatagtt taaattattt cgcactttat
      421 cgaatatttg tttaaaaatg gcaacctgag ccattgtagt accttccatg tgatatgagg
      481 gggcgtagtc tgcacgatta tctaaattgc ttcaatctgg tctaacctgt tttctgagca
      541 attcagtaat gtcactcttt tctttgtttg cttcaggcga aactcttttt tctgagcaca
      601 gtctccggcg gcaggcttca atgacccagg ctgagaaatt cccggaccct ttttgaacaa
      661 gagcgatgtt aatttgttca atcatttggt taggaaagcg gatgttgcgg gttgttgttc
      721 tgcgggttct gttcttcgtt gacatgaggt tgccctgtat tcagtgtcgc tgatttgtat
      781 tgtctgaagt tgtttttacg ttaagttgat gcagatcaat taatacgata cctgcgtcat
      841 aattgattat ttgacgtggt ttgatggcgt agatgcacgt tgtgacatgt agatgataat
      901 tattatcatt ttgcgggtcc tttccggcga tccgacaggt tacggggcgg aaggcgcgcg
      961 ggttttcgct atttatgaca attttccggt ttaaggcgtt tccgttcttc ttcgccgtaa
     1021 cctaatgttt ttatttaaaa caccccctga aaagaaagga aacgacaggt gctgaaaacg
     1081 ggctttttgg cctctgtcgt ttcctttctc tgtttttgtc cgtggaatga acaatggaag
     1141 tcaacaaaaa gcagctggct gacattttcg gtgcgagtat ccgtaccatt cagaactggc
     1201 aggaacaggg aatgcccgtt ctgcgaggcg gtggcaaggg taatgaggtg ctttatgact
     1261 ctgccgccgt tataagatgg tatgccgaaa gggatgctga aattgagaac gaaaagctgc
     1321 gccgggaagt tgaagaactg cggcaggcca gcgagacaga tctccagcca gggactattg
     1381 agtacgaacg ccatcgactt acgcgtgcgc aggccgacgc acaggagctg aaaaatgcca
     1441 gagactccgc tgaagtggtg gaaaccgcat tctgtacttt cgtgctgtcg cggatcgcag
     1501 gtgaaattgc cagtattctc gacgggatcc ccctgtcggt gcagcggcgt tttccggaac
     1561 tggaaaaccg acatgttgat ttcctgaaac gggatatcat caaagccatg aacaaagcag
     1621 ccgcgctgga tgaactgata ccggggttgc tgagtgaata tatcgaacag tcaggttaac
     1681 aggctgcggc attttgtccg cgccgggctt cgctcactgt tcaggccgga gccacagacc
     1741 gccgttgaat gggcggatgc taattactat ctcccgaaag aatccgcata ccaggaaggg
     1801 cgctgggaaa cactgccctt tcagcgggcc atcatgaatg cgatgggcag cgactacatc
     1861 cgtgaggtga atgtggtgaa gtctgcccgt gtcggttatt ccaaaatgct gctgggtgtt
     1921 tatgcctact ttatagagca taagcagcgc aacacactta tctggttgcc gacggatggt
     1981 gatgccgaga actttatgaa aacccacgtt gagccgacca tccgcgatat tccgtcgctg
     2041 ctggcgctgg ctccgtggta tggcaaaaag caccgggata acacgctcac tatgaagcgt
     2101 ttttccaatg gtcgtggctt ctggtgcctg ggcggtaaag cggcaaaaaa ctaccgtgaa
     2161 aagtcggtgg atgtggcggg ttatgatgaa cttgctgcct ttgatgagga tattgaacag
     2221 gaaggctctc cgacgttcct tggcgacaaa cgtattgaag gctcggtctg gccaaagtcc
     2281 atccgtggct ccacgcccaa agtgagaggc acctgccaga ttgagcgtgc agccagtgaa
     2341 tccccgcatt ttatgcgttt tcatgttgcc tgcccgcact gcggggagga gcagtatctt
     2401 aaatttggcg acaaagagac gccgtttggc ctcaaatgga cgccggatga cccctccagc
     2461 gtgttttatc tctgcgagca taatgcctgc gtcatccgcc agcaggagct ggactttact
     2521 gatgcccgtt atatctgcga aaagaccggg atctggaccc gtgatggcat tctctggttt
     2581 tcgtcatccg gtgaagagat tgagccacct gacagtgtga cctttcacat ctggacagcg
     2641 tacagcccgt tcaccacctg ggtgcagatt gtcaaagact ggatgaaaac gaaaggggat
     2701 acgggaaaac gtaaaacctt cgtaaacacc acgctcggtg agacgtggga ggcgaaaatt
     2761 ggcgaacgtc cggatgctga agtgatggca gagcggaaag agcattattc agcgcccgtt
     2821 cctgaccgtg tggcttacct gaccgccggt atcgactccc agcgggatcg ctacgaaatg
     2881 cgcgtatggg gatgggggcc gggtgaggaa agctggctga ttgatcggca gattattatg
     2941 ggccgccacg acgatgaaca gacgctgctg cgtgtggatg aggccatcaa taaaacctat
     3001 acccgccgga atggtgcaga aatgtcgata tcccgtatct gctgggatat tggcgggatt
     3061 gacccgacca ttgtgtatga acgctcgaaa aagcatgggc tgttccgggt gatccccatt
     3121 aaaggggcat ccgtctacgg taagccggtg gccagcatgc ctcgtaagcg aaacaaaaac
     3181 ggggtttacc ttaccgaaat cggtacggat accgcgaaag agcagattta taaccgcttc
     3241 acactgacgc cggaagggga tgaaccgctt cccggtgccg ttcacttccc gaataacccg
     3301 gatatttttg atctgaccga agcgcagcag ctgactgctg aagagcaggt cgaaaaatgg
     3361 gtggatggca ggaaaaaaat actgtgggac agcaaaaagc gacgcaatga ggcgctcgac
     3421 tgcttcgttt atgcgctggc ggcgctgcgc atcagtattt cccgctggca gctggatctc
     3481 agtgcactgc tggcgagcct gcaggaagag gatggtgcag caaccaacaa gaaaacactg
     3541 gcagattacg cccgtgcctt atccggagag gatgaatgac gcgacaggaa gaacttgccg
     3601 ctgcccgtgc ggcactgcat gacctgatga caggtaaacg ggtggcaacg gtacagaaag
     3661 acggacggcg agtggagttt acggccactt ccgtgtctga cctgaaaaaa tacattgcgg
     3721 agctggaggt gcagaccggc atgacacagc gacgcagggg acctgcagga ttttatgtat
     3781 gaaaacgccc accattccca cccttctggg accggacggc atgacatcgc tgcgtgaata
     3841 tgccggttat cacggcggtg gcagcggatt tggtgggcag ttgcgggcgt ggaacccacc
     3901 gggtgaaagt gtggatgcag ccctgctgcc caactttacc cgtggcaatg cccgcgcaga
     3961 cgatctggta cgcaataacg gctatgctgc caacgccatc cagctgcatc aggatcatat
     4021 cgtcgggtct tttttccggc tcagtcatcg cccaagctgg cgctatctgg gcatcgggga
     4081 ggaagacgcc cgtgcctttt cccgcgaggt tgaagcggca tggaaagagt ttgccgagga
     4141 tgactgctgc tgcattgacg ttgagcgaaa acgcacgttt accatgatga ttcgggaagg
     4201 tgtggccatg cacgccttta acggtgaact gttcgttcag gccacctggg ataccagttc
     4261 gtcgcggctt ttccggacac agttccggat ggtcagcccg aagcgcatca gcaacccgaa
     4321 caataccggc gacagccgga actgccgtgc cggtgtgcag attaatgaca gcggtgcggc
     4381 gctgggatat tacgtcagcg aggacggcta tcctggctgg atgccgcaga aatggacatg
     4441 gataccccgt gagttacccg gcgggcgcgc ctcgttcatt cacgtttttg aacccgtgga
     4501 ggacgggcag acccgcggtg caaatgtgtt ttacagcgta atggagcaga tgaagatgct
     4561 cgacacgctg cagaacacgc agctgcagag cgccattgtg aaggcgatgt atgccgccac
     4621 cattgaaagt gagctggata cgcagtcagc gatggatttt attctgggcg cgaacagtaa
     4681 ggagcagcgg gaaaggctga cgggctggat tggtgaaatt gccgcgtatt acgccgcagc
     4741 accggtccgt ctgggaggcg caaaagtgcc gcacctgatg ccgggggact cactgaacct
     4801 gcagacggct caggacacgg ataacggcta ctccgtgttt gagcagtcac tgttgcggta
     4861 tatcgctgcc gggctgggtg tctcgtatga gcaactttcc cggaattacg cccagatgag
     4921 ctactccacg gcacgggcca gtgcgaacga gtcgtgggcg tactttatgg ggcggcgaaa
     4981 attcgtcgca tcccgtcagg cgagccagat gtttctgtgc tggctggaag aggccatcgt
     5041 tcgccgcgtg gtgacgttac cttcaaaagc gcgcttcagc tttcaggaag cccgcagtgc
     5101 ctgggggaac tgcgactgga taggctccgg tcgtatggcc atcgatggtc tgaaagaagt
     5161 tcaggaagcg gtgatgctga tagaagccgg actgagcacc tacgagaaag agtgcgcgaa
     5221 acgcggtgac gactatcagg aaatttttgc ccagcaggtc cgtgaaacga tggagcgccg
     5281 cgcagctggt cttaaaccgc ccgcctgggc ggctgcggca tttgaatccg ggctgcgaca
     5341 atcaacagag gaggagaaga gtgacagcag agctgcgtaa tctcccgcat attgccagca
     5401 tggcttttaa tgagccgctg atgcttgaac ccgcctatgc gcgggttttc ttttgtgcgc
     5461 ttgcaggcca gcttgggatc agctgcctga cggatgcagt atccggcgac agcctgactg
     5521 ccggagaggc acccgcggcg ctggcgttat ccggtgatga tgacggacca cgacaggccc
     5581 gcagttatca ggtaatgaac ggcatcgccg tgctgccggt gtccggcacg ctggtcagcc
     5641 ggacgcgggc gctgcagccg tactcgggga tgaccggtta caacggcatt atcgcccgtc
     5701 tgcaacaggc tgccagcgac ccgatggtgg acggcattct gctcgatatg gacacaccgg
     5761 gcggaatggt ggcgggagca tttgactgcg ctgacatcat cgcccgtgtg cgtgacataa
     5821 agccggtatg ggcgctggcc aatgacatga actgcagcgc aggtcagctg cttgccagtg
     5881 ccgcctcccg gcgtctggtc acgcagaccg cccggacagg ctccatcggc gtcatgatgg
     5941 ctcacagtaa ttacggcgct gcactggaga aacagggcgt ggaaatcacg ctgatttaca
     6001 gcggcagcca taaggtggat ggcaacccct acagccatct accggatgat gtccgggaaa
     6061 cattgcagtc ccggatggat gcaacccgcc ggatgtttgc acagaaggtg tcggcatata
     6121 ccggcctgtc cgtgcaggct gtgctggata ccgaggctgc agtgtacagc ggtcaggagg
     6181 ccattgatgc cggactggct gatgaacttg ttaacagtac cgatgcgatc accgtcatgc
     6241 gtgatgcact ggatgcacgt aaatcccgtc tctcaggagg gcgaatgacc aaagagactc
     6301 aatcaacaac tgtttcagcc actgcttcgc aggctgacgt tactggcgtg gtgcaagcga
     6361 cggagggcga gaacgccagc gcggcgcagc cggacgtgaa cgcgcagatc accgcagcgg
     6421 ttgcggcaga aaacagtcgc attatgggga tcctcaactg tgaggaggct cacggacgcg
     6481 aagaacaggc acgcgtgctg gccgaaaccc ccggtatgac cgtggaaacg gcccgccgta
     6541 ttctggccgc agcaccacag agtgcacaga cgcgcagtga cactgcgctg gatcgtctga
     6601 tgcagggggc accggcaccg ctggctgcag gtaacccggc atctgatgcc gttaacgatt
     6661 tgctgaacac accagtgtaa gggatgttta tgacgagcaa agaaaccttt acccattacc
     6721 agccgctggg caatagtgac ccggctcata ccgcaaccgc gcccggcgga ttgagtgcga
     6781 aagcgcctgc aatgaccccg ctgatgctgg acacctccac ccgtaagctg gttgcgtggg
     6841 atggcaccac cgacggtgct gccgttggca ttcttgcggt tgctgctgac cagaccagca
     6901 ccacactgac gttctacaag tccggcacgt tccgttatga ggatgtgctc tggccggagg
     6961 ctgccagcga cgagacgaaa aaacggaccg tgtttgccgg aacggcaatc agcatcgttt
     7021 aactttaccc ttcatcacta aaggccgcct gtgtggcttt ttttacggga tttttttatg
     7081 tcgatgtaca caaccgccca gctgctggcg gcaaatgagc agaaatttaa gtttgatccg
     7141 ctgtttctgc gtctcttttt ccgtgagagc tatcccttca ctacggagaa agtctatctc
     7201 tcacaaattc cgggactggt aaacatggcg ctgtacgttt cgccgattgt ttccggtgag
     7261 gttatccgtt cccgtggcgg ctccacctct gaatttacgc cgggatatgt caaacccaag
     7321 catgaggtga atccgcagat gaccctgcgt cgcctgccgg atgaagatcc gcagaatctg
     7381 gcggacccgg cttaccgccg ccgtcgcatc atcatgcaga acatgcgtga cgaagagctg
     7441 gccattgctc aggtcgaaga gatgcaggca gtttctgccg tgctcaaggg caaatacacc
     7501 atgaccggtg aagccttcga tccggttgag gtggatatgg gccgcagtgc ggcgaacaac
     7561 atcacacagt ccggcgtcac ggagtggagc aagcgtgaca agtccacgta tgacccgacc
     7621 gacgatatcg aagcctacgc gctgaacgcc agcggtgtgg tgaatatcat cgtgtttgac
     7681 ccgaaaggct gggcgctgtt ccgttccttc aaagccgtca aggagaagct ggatacccgt
     7741 cgcggctctc attccgagct ggagacagcg gtaaaagacc tgggcaaagc ggtgtcctat
     7801 aagggaatgt atggcgatgt ggccatcgtc gtgtattccg gacagtacgt ggaaaacggc
     7861 gtcaaaaaga acttcctgcc ggacaacacg atggtgctgg ggaacactca ggctcgcggt
     7921 ctgcgcacct atggctgcat tcaggatgcg gacgcacagc gcgaaggcat taacgcttct
     7981 gcccgttacc cgaaaaactg ggtgaccacc ggcgatccgg cgcgtgagtt caccatgatt
     8041 cagtcagcac cgctgatgct gctggctgat cctgatgagt tcgtgtccgt tcaactggcg
     8101 taatcatggc ccttcggggc cattttctct ctgtggagga gttcatgacg aaagatgaac
     8161 tgattgcccg tcttcaggag ctgggtgagc aattgaaccg cgatgtcagt ttgacgggaa
     8221 cgaaagaaga actggcgctt cgtgtggcag agctggaaga agagcttgat gacacggatg
     8281 aaactgccgg tcaggacacc cctctcagcc gggaaaatgt gctgaccggg catgaaaatg
     8341 aggtggtatc agcgcagccg gataccgtga ttcaggatac ggctgaactg gtcacggtcg
     8401 tggcactggt gacgctgcat actgatgcac ttcacgccac gcgggataaa cctgtggcat
     8461 ttgtgctgcc gggaacggcg tttcgtgtct ctgccggtgt ggcagccgaa atgacagagc
     8521 gcggcctggc cagaatgcaa taacgggagg cgctgtggct gatttcgata acctgttcga
     8581 tgctgccatt gcccgcgccg atgaaacgat acgcgggtac atgggaacgt cagccaccat
     8641 gacatccggt gagcagtcag gtgcggtgat acgtggtgtt tttgatgacc ctgaaaatat
     8701 cagctatgcc ggacagggcg tgcgcgttga aggctccagc ccgtccctgt ttgtccggac
     8761 tgatgaggtg cggcagctgc ggcgtggaga cacgctgacc atcggtgagg aaaatttctg
     8821 ggtagatcgg gtttcgccgg atgatggcgg aagttgtcat ctctggcttg gacggggcgt
     8881 accgcctgcc gttaaccgtc gccgctgaaa gggggatgta tggccataaa aggtcttgag
     8941 caggccgttg aaaacctcag ccgtatcagc aaaacggcgg tgcccggtgc cgccgcaatg
     9001 gccattaacc gcgttgcttc atccgcgata tcgcagtctg cgtcacaggt tgcccgtgag
     9061 acaaaggtac gccggaaact ggtaaaggaa agggccaggc tgaaaagggc cacggtcaaa
     9121 aatccgcagg ccagaatcaa agttaaccgg ggggatttgc ccgtaatcaa gctgggtaat
     9181 gcgcgggttg tcctgtcccg ccgcaggcgt cataaaaagg ggcagcgttc atccctgaaa
     9241 ggtggcggca gcgtgcttgt ggtgggtaac cgtcgtattc ccggcgcgtt tattcagcaa
     9301 ctgaaaaacg gccggtggca tgttatgcag cgtgtggccg ggaaaaaccg ttaccccatt
     9361 gatgtggtga aaatcccgat ggcggtgccg ctgaccacgg cgtttaaaca gaatattgaa
     9421 cggatacggc gtgaacgtct tccgaaagag ctgggctatg cgctgcagca tcaactgaga
     9481 atggtaataa agcgatgaaa catactgaac tccgtgcagc cgtactggat gcactggaga
     9541 agcatgacac cggggcgacg ctttttgatg gtcgccccgc tgtttttgat gaggaagatt
     9601 ttccggcaat tgccgtttat ctcaccggcg ctgaatacac gggcgaagag ctggacagcg
     9661 atacctggca ggcggagctg catattgaag ttttcctgcc tgctcaggtg ccggattcag
     9721 agctggattc gtggatggag tcccggattt atccggtgat gagcgatatc ccggcactgt
     9781 cagatttgat caccagtatg gtggccagcg gctatgacta ccggcgcgac gatgatgcgg
     9841 gcctgtggag ttcagccgat ctgacttatg tcattatcta tgaaatgtga ggacgctatg
     9901 cctgtaccaa atcctacaat gccggtgaaa ggtgccggga ccaccctgtg ggtttataag
     9961 gggagcggtg acccttatgc gaacccgctt tcagacgttg actggtcgcg tctggcaaaa
    10021 gttaaagacc tgacgcccgg cgaactgacc gctgagtcct atgacgacag ctatctcgat
    10081 gatgaagatg cagactggac tgcgaccggg caggggcaga aatctgccgg agataccagc
    10141 ttcacgctgg cgtggatgcc cggagagcag gggcagcagg cgctgctggc gtggtttaat
    10201 gaaggtgata cccgtgccta taaaatccgc ttcccgaacg gcacggtcga tgtgttccgt
    10261 ggctgggtca gcagtatcgg taaggcggtg acggcgaagg aggtgattac ccgcacggtg
    10321 aaggtcacca atgtgggacg tccgtcgatg gcagaagatc gcagcacggt gacggcgacg
    10381 accggcatga cggtgacacc cgccagtgct tccgtagtga aagggcagag caccacgctg
    10441 accgtggcat tccagccgga tggcgcaacc gacaagagct tccgtgcggt gtctgcggat
    10501 aaaacaaaag ccaccgtgtc ggtcagtggt atgaccatca ccgtgaaagg tgttgctgca
    10561 ggcaaggtca acattccggt cgtatctggt aatggtgagt ttgctgcggt tgcagaaatc
    10621 aacgtcaccg ccagttaatc cggagagtca gcgatgttcc tgaaaaccga atcatttgaa
    10681 cataacggtg tgaccgtcac gctttctgaa ctgtcagccc tgcagcgtat tgagcatctc
    10741 gacctgatga aacggcaggc agaacaggcg gagtcagaca gcaaccggaa gtttactgtg
    10801 gaagacgcca tcagaaccgg cgcttttgtg gttgcgatgt ccctgtggca taaccatccg
    10861 aagaagacgc agatgccgtc catgaatgaa gccgttaaac agattgagca ggaagtgctt
    10921 accacctggc ccacagaggc aatttctcat gctgaaaacg tggtgtaccg gctgtccggt
    10981 atgtatgagt ttgtggtgaa tgatgctcct gaacaggcag aggacgccgg gcctgcagag
    11041 cctgtttctg cgggaaagtg ttcgacggtg agctgagttt tgccctgaaa ctggcgcgtg
    11101 agatggggcg acccgactgg cgcgccatgc ttgccgggat gtcatccacg gagtatgccg
    11161 actggcaccg cttttacagt acccattatt ttcatgatgt tctgctggat atgcactttt
    11221 ccgggctgac gtacaccgtg ctcagcctgt ttttcagcga tccggagatg catccgctgg
    11281 atttcagtct gctgaaccgg cgcgaggctg acgaagagcc tgaagatgat gtgctgatgc
    11341 agaaagcggc agggcttgcc ggaggtgtcc gctttggccc ggacgggaat gaagttatcc
    11401 ccgcttcccc ggatgtggcg gacatgacgg aggatgacgt aatgctgatg acagtatcag
    11461 aagggatcgc aggaggagtc cggtatggct gaaccggtag gcgatctggt cgttgatttg
    11521 agtctggatg cggccagatt tgacgagcag atggccagag tcaggcgtca tttttccggt
    11581 acggaaagtg atgcgaaaaa aacagcggca gtcgttgaac agtcgctgag ccgacaggcg
    11641 ctggctgcac agaaagcggg gatttccgtc gggcagtata aagccgccat gcgtatgctg
    11701 cctgcacagt tcaccgacgt ggccacgcag cttgcagggg ggcagaatcc ctggctgatc
    11761 ctgctgcaac agggtggtca ggttaaggac tccttcggcg ggatgatccc catgttcagg
    11821 gggcttgccg gcgcgatcac cctgccgatg gtgggggcca cctcgctggc ggtggcgacc
    11881 ggtgcgctgg cgtatgcctg gtatcagggc aactcaaccc tgtccgattt caacaaaacg
    11941 ctggtccttt ccggtaatca ggcgggactg acggcagatc gtatgctggt cctgtccaga
    12001 gccgggcagg cggcagggct gacgtttaac cagaccagcg agtcactcag cgcactggtt
    12061 aaggcggggg taagcggtga ggctcagatt gcgtccatca gccagagtgt ggcgcgtttc
    12121 tcctatgcat ccggcgtgga ggtggacaag gtcgctgaag ccttcgggaa gctgaccaca
    12181 gacccgacgt cggggctgac ggcgatggca cgccagttcc ataacgtgac ggcggagcag
    12241 attgcgtatg ttgctcagtt gcagcgttcc ggcgatgaag ccggggcatt gcaggcggcg
    12301 aacgaggccg caacgaaagg gtttgatgac cagacccgcc gcctgaaaga gaacatgggc
    12361 acgctggaga cctgggcaga caggactgca cgtgcattca aatccatgtg ggatgcggtg
    12421 ctggatattg gtcgtcctga taccgcgcag gagatgctga ttaaggcaga ggctgcgttt
    12481 aagaaagcag acgacatctg gaatctgcgc aaggatgatt atttcgttaa cgatgaagca
    12541 cgggcgcgtt actgggatga tcgtgaaaaa aaacgcctgg agcgggatgc agcccaaaag
    12601 agggtagatc aacagcgtca acaggacaaa aatgcgcagc agcagagcga taccgaagcg
    12661 tcacggctga aatataccga agaggcgcag aaggcttacg aacggctgca gacgccgctg
    12721 gagaaatata ccgcccgtca ggaagaactg aacaaggcac tgaaagacgg aaaaatcctg
    12781 caggcagatt acaacacgct gatggcggcg gcgaaaaagg attatgaagc gacgctgaaa
    12841 aagccgaaac agtccggcgt gaaggtgtat gcgggcgatc gtcaggaaga cagtgctcat
    12901 gcggccctgc tgacgcttca ggcagaactc cggacgctgg agaagcatgc cggagcgaat
    12961 gagaaaatca gccagcagcg ccgggatttg tggaaggcag aaagtcagtt cgcggtactg
    13021 gaggaggcgg cacaacgtcg ccagctgtcc gcacaggaga aatccctgct ggcgcataaa
    13081 gacgagacgc tggagtacaa acgccagctg gctgcacttg gtgacaaggt tacgtatcag
    13141 gagcacctga atgcgctggc gcagcaggcg gataaattcg cacagcagca acgggcaaaa
    13201 cgggcagcca ttgatgcgaa aaaccggggg ctgactgacc ggcaggcagc gcgggaagcc
    13261 acggaacagc gcctgaagga acagtatggc gataatcctc tggcgctgaa taacgtcatg
    13321 tcagagcaga aaaagacctg ggcggctgaa gaccagcttc gcgggagctg gatggcaggc
    13381 ctgaagtccg gctggagtga gtgggaagag agcgccacgg acagtatgtc acaggttaaa
    13441 agtgcagcca cgcagacctt tgatggtatt gcacagaata tggcggcgat gctgaccggc
    13501 agtgaacaga actggcgcag cttcacccgc tccgtgctgt ccatgatgac agaaattctg
    13561 cttaagcagg caatggtggg gattgtcggg agtatcggca gcgccattgg cggggctgtt
    13621 ggtggcggcg catccgcgtc aggtggtaca gccattcagg ccgctgcggt gaaattccat
    13681 tttgcaaccg ggggatttac gggaaccggc ggcaaatatg agccagcggg gattgttcac
    13741 cgtggtgaat ttgtcttcac gaaggaggca accagccgga ttggcgtggg gaatctctac
    13801 cggctgatgc gcggctatgc ggaaggtggt tatgtgggcg gtgccggaag tccggcgcag
    13861 atgcggcggg ctgaaggcat taattttaat cagaacaatc acgtggtgat tcagaacgac
    13921 ggtacgaatg gtctgccagg tccacagatg atgaaggccg tgtatgacat ggcccgcaag
    13981 ggtgcccgtg atgaaattca gacacagatg cgtgatggtg gcctgttctc cggaggtgga
    14041 cgatgaaaac cttccgctgg aaagtgaaac ccggtatgga tgtggcttcg gccccttctg
    14101 taagaaaggt gcgctttggt gatggctatt cccagcgagc gcctgccggg ctgaatgcca
    14161 acctgaaaac gtacagcgtg acgctttctg tcccccgtga ggaggccatg gtactggagt
    14221 cgtttctgga agagcacggg ggctggaaag cctttctgtg gacgccgcct tatgagtggc
    14281 ggcagataaa ggtgacctgc gcaaaatggt cgtcgcgggt cagtatgctg cgtgttgagt
    14341 tcagcgcaga gtttgaacag gtggtgaact gatgcaggat atccggcagg aaacactgaa
    14401 tgaatgcacc cgtgcggagc agtcggccag cgtggtgctc tgggaaatcg atctgacaga
    14461 ggtcggtgga gaacgttatt ttttctgtaa tgagcagaac gaaaaaggtg agccggtcac
    14521 ctggcagggg cgacagtatc agccgtatcc cattcagggg agtggttttg aactgaatgg
    14581 caaaggcacc agtacgcgcc ccacgctgat ggtttctaac ctgtacggta tggtcaccgg
    14641 gatggcggaa gatctacaga gtctggtcgg cggaacggtg gtccggcgta aggtttacgc
    14701 ccgttttctg gatgcggtga acttcgtcaa cggaaacagt gacgccgatc cggagcagga
    14761 ggtgatcagc cgctggcgca ttgagcagtg cagcgaactg agcgcggtga gtgcctcttt
    14821 tgtactgtcc acgccgacgg aaacagatgg cgctgttttt ccgggacgta tcatgctggc
    14881 caacacctgc acctggacct atcgcggcga tgagtgcggt tatcacggtc cggcggtcgc
    14941 ggatgaatat gaccagccaa cgtccgacat cacgaaggat aaatgcagca aatgcctgag
    15001 tggctgtaag tttcgcaata acgtcggcaa ctttggcggc ttcctttcca ttaacaaact
    15061 ttcgcagtaa atcccatgac agagacagaa tcagcgattc tggcgcacgc ccggcgatgt
    15121 gctccagcgg agtcgtgcgg cttcgtggta agaacgccgg agggggaaag atatttcccc
    15181 tgcgtgaata tctccggtga gccggaggcg tatttccgta tgtcgccgga agactggctg
    15241 caggcagaga tgcagggtga gattgtggca ctggtccaca gccaccccgg tggtctgccc
    15301 tggctgagtg aggccgaccg gcggctgcag gtgcagagtg atttgccgtg gtggctggtc
    15361 tgccgggggg cgattcacaa g
//
LOCUS       DAJVRF010000095        15269 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJVRF010000095 DAJVRF010000000
VERSION     DAJVRF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15269
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.095"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..119)
                     /locus_tag="OQN13_002734"
     CDS             complement(<1..119)
                     /locus_tag="OQN13_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HCS1616046.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYG"
     gene            complement(124..309)
                     /locus_tag="OQN13_002735"
     CDS             complement(124..309)
                     /locus_tag="OQN13_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCS1616047.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(306..2945)
                     /locus_tag="OQN13_002736"
     CDS             complement(306..2945)
                     /locus_tag="OQN13_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCS1616048.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3153..4142
                     /gene="ldhA"
                     /locus_tag="OQN13_002737"
     CDS             3153..4142
                     /gene="ldhA"
                     /locus_tag="OQN13_002737"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS1616049.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4253..4675
                     /gene="hslJ"
                     /locus_tag="OQN13_002738"
     CDS             4253..4675
                     /gene="hslJ"
                     /locus_tag="OQN13_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCS1616050.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4672..4938)
                     /locus_tag="OQN13_002739"
     CDS             complement(4672..4938)
                     /locus_tag="OQN13_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCS1616051.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5212..8736
                     /gene="nifJ"
                     /locus_tag="OQN13_002740"
     CDS             5212..8736
                     /gene="nifJ"
                     /locus_tag="OQN13_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCS1616052.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9102..10235
                     /gene="ompN"
                     /locus_tag="OQN13_002741"
     CDS             9102..10235
                     /gene="ompN"
                     /locus_tag="OQN13_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCS1616053.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10376..10810
                     /gene="uspF"
                     /locus_tag="OQN13_002742"
     CDS             10376..10810
                     /gene="uspF"
                     /locus_tag="OQN13_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCS1616054.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11396..12310
                     /gene="sitA"
                     /locus_tag="OQN13_002743"
     CDS             11396..12310
                     /gene="sitA"
                     /locus_tag="OQN13_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCS1616055.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12310..13137
                     /gene="sitB"
                     /locus_tag="OQN13_002744"
     CDS             12310..13137
                     /gene="sitB"
                     /locus_tag="OQN13_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCS1616056.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            13134..13991
                     /gene="sitC"
                     /locus_tag="OQN13_002745"
     CDS             13134..13991
                     /gene="sitC"
                     /locus_tag="OQN13_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCS1616057.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            13988..14845
                     /gene="sitD"
                     /locus_tag="OQN13_002746"
     CDS             13988..14845
                     /gene="sitD"
                     /locus_tag="OQN13_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCS1616058.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(15046..>15269)
                     /locus_tag="OQN13_002747"
     CDS             complement(15046..>15269)
                     /locus_tag="OQN13_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCS1616059.1"
                     /translation="MAAKSDIVRDVMTWGDADNQQVKQSMPELEELAAAMAQAQVERN
                     DEIYRRQRELKEELNSLKDLNSVRNFIVE"
BASE COUNT         3833 a         3866 c         3687 g         3883 t
ORIGIN      
        1 ccataaaatg tttcacctac ccgcccctga gttctggctt catccagagt caatgccatt
       61 acattgctgc ttaccagccc aacaagaaac gcacaaagaa gtaatgtttt cttcatcatc
      121 gcctcagaaa agatcgctac gagtttcaag cagctcttcg acatctttgt ctgccttgat
      181 gatgatctca tgctcaattt taacgttcat attgatagtg atcggttcct tgggcgcagc
      241 gacttcaata cgcggcgtac agccaaccag cataaaagat gacgtcaacg cagccagtaa
      301 aattttcatt gtttttcctc acactctttt ccttgcggac agtcatttcc cggcagacgt
      361 gcgttctgct ccaaccatgc ctggagatta tcgccaaagc gtaaactgcg ccacagcgta
      421 aaaatattct cttcatgatg gtaattaagg tttaccgtac cgcttttacc atcaacgcga
      481 ctggtacctg taatgttggc ctgcatggtt aataaaccga gattatctaa gttaattttt
      541 gtcgatgacc ggctaatttc catatagcgc aaccagttaa ttgctgaacc cgcagtcata
      601 ttgtctttca ccaccgcatc tgcggtgtct ttatccagcc gcaaggtcat tggcccgcta
      661 tttgccagcc aaccatcttt cactatccat ttttcgttgt ttagccataa cggcaatgca
      721 ccactaaatg ccccggacat ggcgaattgt ttcggattga cggcgctaac cagttcgctg
      781 gatgagagat tattcagccg caacagcgcc gggtcatgtt gcggcatacg taattgtttc
      841 atcagtacgt taccgcctaa cacatcgacg ctaacatcag tcaacagcaa gggttcttct
      901 tcagtccacg gataacgccc ttgcaaatca gccgtaatat ttttcgctgt caccagatta
      961 atcacttcgg caattcgcaa cgtaacgggg ccgcgagtcc ccagatgcca ggctccatcg
     1021 gcaaaacgga aaggcaggac aaaatcgaca ccgttaacct gattatctgg catccaggca
     1081 ctaccgcctt tcaacacgcc gtgtcctccc gcgcggaatc cttgttcagg cgcagcagaa
     1141 aatgcaacct gagcatacag ttccccatcg cgtaagttca tcttccagtc gggtggcacc
     1201 agcggctgga atacagtcag tgattgtttt ggccaccagg cgttaccgcg cagacgaata
     1261 ccgtcccagc gaccatttac ccgaaccggg ccaatctcac cagcatgtaa atcgccttta
     1321 aagaggaaat acgtcggatc gcgcccatcg acgctaaatt ttaaggttga tggcggtaac
     1381 acactgccgc cagtgaacag cgtttgcccg gcgtccagtg acagcgcgcc gctgaaggag
     1441 ggatgctgtg cgtcacgtac ccagacgacg ggcttgtcga gaattaatcg cggcttttct
     1501 accgtcatcg taccgtattg taactggtcg aaaccggtgg agaggtcggt cagcgtaatc
     1561 gtgctgtcat gccattcacc ttttcctgcg acatcccagg tggcatttat cggtgtaaaa
     1621 ctgccttttc cccagtagcg ccactgccag cggcccgcgt cagggagaaa atcattcgcc
     1681 agcccatcca tatgcagcac gaaatcgccc agttcatatt catgcgcctg caaaatggcc
     1741 tgcaaacgtc cgtcaacacc acgttgggtg acttttacac ccgctaaagg ccagcggatt
     1801 tcatcgatgt ccagcgaatc gatgactctt cccttcgaac gtaataacgc gcctggctca
     1861 aaggccagcg ttgggtcagt cagacttcca cttagctgcg cgggtaagcg ggcatataaa
     1921 atgaggtccg cctgtttcgc ttcaccggtc agctgcagag gcagctggct gttatccatg
     1981 cttaattttc ctgggccaaa atttagcacc gcgttgcctt tacccgcttg cccctgggtc
     2041 agcacactca gtcgtccgct gatcagagcg ttctcaagcc ctgcctgcca gttgtcgact
     2101 ttgacaccca gtcggccact caaaggaaaa cctgcatacg gccaactcca gcgaccatcg
     2161 cttacggtca attgttgacg agtaatttgc cacggcaaat cgagcaacgg atcgccgtta
     2221 tcccgtgcca gcacaatcaa ttgcccgcta ttttcctgcc agtccagctc tgcatccacc
     2281 agtgacggtt cctgcggcaa gtttaacgtc gcagtagcat gcccacttac aggaagtcca
     2341 tccggcacga gcggcatagt aaattccccc accagtttaa ccggcggctg attttcaaac
     2401 gcgatgacat ccagttcgct gactgtaagt tgttgccctt tcagctggcc ttgaaattta
     2461 actttttcgc cctgataacg cagttgctga atatcagagg ttaatgcgag agagagtttt
     2521 ccctgccatt cctgccaggg agaaaaaatc agtttatcga tattgatcca ggtgttaggc
     2581 agcatggact gccactgcgc gagggttttt ggagcagctg gcgattgctc cgtctgcggc
     2641 aatttcgcca gacaagcaga atcaagttct accatgccga cgttcaataa ccagcggctg
     2701 ggatgtgaaa ggctggcgtt ggtgatatgc gcaagctgac aatctcccac cagataacgg
     2761 agatcgggaa tgattaaacc tttacgcgta atgcgtgggc tttcatctaa tgcaatacgt
     2821 gtcccgagcg gtagccagat gcccgccagc gtgggaaccc acagcccgag cgtcatcagc
     2881 agcgtcaacg gcacaagaat aatcagtaat aacagcgcga gaacggcttt atatttaccc
     2941 agcatgggta gttaatatcc tgatttagcg aaaaattaag cattcaatac gggtattgtg
     3001 gcatgtttaa ccgttcagtt gaaggttgcg cctacactaa gcattgttgt tgatgaattt
     3061 ttcaatatcg ccatagcttt caattatatt tgaaattttg taaaatattt ttagtagctt
     3121 aaatgtgatt caacatcact ggagaaagtc ttatgaaact cgccgtttat agcacaaaac
     3181 agtacgacaa gaagtacctg caacaggtga acgagtcctt tggctttgag ctggaatttt
     3241 ttgactttct gctgacggaa aaaaccgcta aaactgccaa tggctgcgaa gcggtatgta
     3301 ttttcgtaaa cgatgacggc agccgcccgg tgctggaaga gctgaaaaag cacggcgtta
     3361 aatatatcgc cctgcgctgt gccggtttca ataacgtcga ccttgacgcg gcaaaagaac
     3421 tggggctgaa agtagtccgt gttccagcct atgatccaga ggccgttgct gaacacgcca
     3481 tcggtatgat gatgacgctg aaccgccgta ttcaccgcgc gtatcagcgt acccgtgacg
     3541 ctaacttctc tctggaaggt ctgaccggct ttactatgta tggcaaaacg gcaggcgtta
     3601 tcggtaccgg taaaatcggt gtggcgatgc tgcgcattct gaaaggtttt ggtatgcgtc
     3661 tgctggcgtt cgatccgtat ccaagtgcag cggcgctgga actcggtgtg gagtatgtcg
     3721 atctgccaac cctgttctct gaatcagacg ttatctctct gcactgcccg ctgacaccgg
     3781 aaaactacca tctgttgaac gaagccgcct tcgatcagat gaaaaatggc gtgatgatcg
     3841 tcaataccag tcgcggtgca ttgattgatt ctcaggcagc aattgaagcg ctgaaaaatc
     3901 agaaaattgg gtcgttgggt atggacgtct atgagaacga acgcgatcta ttctttgaag
     3961 ataaatccaa cgacgtgatc caggatgacg tattccgtcg cctgtctgcc tgccacaacg
     4021 tgctgtttac cgggcaccag gcattcctga cagcagaagc tctgaccagt atttctcaga
     4081 ctacgctgca aaacttaagc aatctggaaa aaggcgaaac ctgcccgaac gaactggttt
     4141 aatcttgccg ctcccctgca ttccagggga gcttattcag ataatcccca aagacctttc
     4201 atcttctatt cttaaaatag ccctgagtca gaaactgtaa ttgagaacca caatgaagaa
     4261 agtagccgcg tttgttgcgc taagcctgct gatggcggga tgtgtaagta atgacaaaat
     4321 tgctgttacg ccagaacagc tacagcatca tcgctttgtg ctggaaagcg taaacggtaa
     4381 gcccgtgacc agcgataaaa atccgccaga aatcagcttt ggtgaaaaaa tgatgatttc
     4441 cggcagcatg tgtaaccgct ttagcggtga aggcaaactg tctaatggtg aactgacagc
     4501 caaagggctg gcaatgaccc gtatgatgtg cgctaacccg cagcttaatg aactcgataa
     4561 caccattagc gaaatgctga aagaaggtgc acaagtggat ctgaccgcga accagttaac
     4621 gctggcgacc gcgaaacaga cattaactta taagctggcg gatttaatga attaatagct
     4681 gccacagctc ccggcggcaa gtgactgttc gctacagcgt ttgccgttgg gtaatgcaca
     4741 catcccaatc gccgtaccat ccagttgacg ggcaacagaa agcgaaccgc cgatcattgc
     4801 acaatttgct tctccactac tggacatcga cgcttttaaa cctggcgcta cgtgcgccgc
     4861 agtcgcctgc tgaacaggtt cactactaca cgccgacaac aataaagcgg cacaccctac
     4921 ccaaaacgct gctcgcatct cttcttcctc tgatcttcaa gccaaacgac accgccataa
     4981 ataataggca gcacagaggg cggcgtcgag agctgtcctg cgcgttgccc cgcattttta
     5041 cttttttatg gctatttttt tgccctctgt ttgatcaaaa cattcattac gctgatgtgg
     5101 gggacacaaa agcgaaaatg cagaagaaag ccatttgcta aaattgaagg attactactg
     5161 ggcgcgcagc aatttcgtgc gcccctcatt tgcgcaatgt aagggtgtca tatgattact
     5221 attgatggta atggcgcggt tgcttcggtg gcgtttcgca ccagtgaagt tatcgccatc
     5281 taccctatta cccccagttc cacgatggca gaacaggctg atgcctgggc cggaaacggc
     5341 ttgaagaacg tttggggaga cacaccatgc gtggttgaaa tgcagtcgga agcgggtgct
     5401 atcgctaccg tgcatggcgc tttgcagacg ggtgcccttt caacatcgtt tacgtcatcg
     5461 cagggtttgc tgctgatgat cccgacactg tacaaactgg caggcgaact cacaccgttt
     5521 gtcctgcatg tagcggcacg taccgttgcc acacatgcac tctctatttt tggcgatcat
     5581 tccgacgtca tggcggtgcg ccagacgggt tgcgcgatgt tgtgtgcggc aaacgtccag
     5641 gaagcgcaag actttgctct catttcgcac atcgcgacgc tgaaaagccg cgtgccattt
     5701 attcatttct ttgatggttt ccgcacgtcc cacgaaatca acaaaattgt cccgctggcc
     5761 gatgacacga ttcttgatct catgccgcag gccgaaattg atgctcatcg cgcccgggcg
     5821 ctcaacccgg aacatccggt gatccgtggt acgtccgcca atcctgacac ttatttccag
     5881 tctcgcgaag ccaccaaccc ctggtacaac gcagtctatg accatgttga acaggcgatg
     5941 aatgatttct ctgccgcgac aggtcgtcag tatcagccgt ttgaatatta cgggcatccg
     6001 caagctgaac gggtgattat cctgatgggc tcagccattg gcacctgtga agaagtggtt
     6061 gatgaattgc taacccgtgg cgaaaaagtc ggcgtgctga aagttcgcct gtaccgcccc
     6121 ttctcagcca aacacttgct acaaactcta ccgggatccg tacgcagcgt ggcggtactg
     6181 gacagaacca aagaacccgg tgcccaggca gaaccgctct atctggatgt gatgaccgca
     6241 ctggcagaag cctttaataa tggcaagcgc gaaactctgc cccgtgtcat tggtgggcgc
     6301 tatggtcttt catccaaaga atttggcccg gactgtgtac tggcggtatt tgccgagctc
     6361 aacgcggcta aaccgaaagc gcgctttacg gttggtattt acgatgatgt aaccaatctg
     6421 tccctgccgt tgccggaaaa cacccagcca aactcggcga aacttgaagc cttgttttat
     6481 ggccttggta gtgatggcag cgtttccgcg accaaaaaca atatcaagat tatcggtaat
     6541 tccacgccgt ggtacgcaca gggctatttt gtttacgact ccaaaaaagc gggcggcctg
     6601 acggtttctc accttcgcgt gagcgaacag ccgattcgtt ccgcttatct catttcccag
     6661 gctgattttg ttggctgcca ccagttgcag tttatcgata aatatcagat ggctgagcgt
     6721 ttaaaacctg gcggcatttt cctgctcaac acgccgtaca gcgcagatga agtgtggtcg
     6781 cgcttgccgc aagaagttca ggccgtgtta aaccagaaaa aagcgcgctt ctatgtgatt
     6841 aacgcggcga aaatcgcccg caaatgtggc ctggcggccc gtattaatac cgtcatgcag
     6901 atggcttttt tccatctgac gcaaattctg cctggcgata gcgccctcgc agaattgcag
     6961 ggtgcgattg ccaaaagtta cagtagcaaa ggccaggatc tggtggaacg caactggcag
     7021 gctctggcgc tggcgcgtga atccgtagaa gaagttccgt tgcaaccggt aaatccgcac
     7081 agcgccaatc gaccgccagt ggtttccgat gccgcccctg atttcgtgaa aaccgtaacc
     7141 gcagcgatgc tcgcagggct tggtgatgcc ctccccgttt cggcgctgcc gccagacggc
     7201 acctggccga tgggcaccac tcgctgggaa aaacgcaata tcgccgaaga gatccccatc
     7261 tggaaagagg aactctgtac ccaatgtaac cactgcgtcg cagcttgccc acactcagct
     7321 attcgcgcaa aagtggtacc acccgaagcg atggaaaacg cccctgccag cctgcattcg
     7381 ctggatgtga aatcgcgtga tatgcgcggg cagaaatatg tcttgcaggt ggctccggaa
     7441 gattgcaccg gttgtaacct gtgcgtcgaa gtttgcccgg cgaaagaccg tcagaatcca
     7501 gagattaaag ccatcaatat gatgtcgcgc ctggaacatg tcgaagaaga gaaaatcaat
     7561 tacgatttct tcctcaacct gccagaaatc gaccgtagca aactggaacg tattgatatt
     7621 cgtacatcgc agctgattac accgctgttt gaatattccg gtgcttgctc cggttgtggc
     7681 gagacgccgt atattaaatt gctgactcag ctctatggcg accggatgtt gatcgctaac
     7741 gccaccggct gttcttcaat ttatggcggt aacctgccct ctacaccgta taccaccgat
     7801 gccaacggtc gtgggccggc atgggcgaac tctctatttg aagataatgc cgaatttggc
     7861 cttggtttcc gcctgacggt cgatcaacac cgtgtccgcg tgctgcgtct gctggatcaa
     7921 tttgccgata aaatcccggc ggaattactg acggcgttga aatcagacgc tacgccagag
     7981 gttcgtcgag aacaggtcgc agctttacgc cagcaactca acgatgttgc cgaagcacat
     8041 gaactgctac gtgatgcaga tgcactggtg gaaaaatcaa tctggctgat tggtggtgat
     8101 ggctgggctt acgatatcgg ctttggcggt ctggatcatg tattgagttt gacggaaaac
     8161 gtcaacattc tggtgctgga tacgcaatgc tattccaaca ccggtggtca ggcgtcgaaa
     8221 gcgacaccgc tgggtgcagt aactaaattt ggcgagcacg gcaaacgtaa agcgcgtaaa
     8281 gatcttggcg tcagtatgat gatgtacggt catgtttatg tggcgcagat ttctctcggc
     8341 gcgcagctga accagacggt gaaagcgatt caggaagcgg aagcgtatcc gggaccatcg
     8401 ctgatcattg cttatagccc gtgtgaagag catggttacg atctggcact cagccatgac
     8461 cagatgcgcc aactcacagc taccggcttc tggccgctat atcgcttcga tcctcgtcgt
     8521 gccgatgaag gcaaactgcc gctggcgctg gattcacgcc cgccgtcaga agcactggaa
     8581 gaaacgttac ttcacgagca acgtttccgt cgtctgaatt cgcagcagcc agaagtggca
     8641 gaacagttat ggaaagatgc tgcagctgat ttgcaaaaac gctatgactt cctggcacaa
     8701 atggccggaa aagcggaaaa aagcaacacc gattaaatgc tctggataag gattacccaa
     8761 ttctaaaaaa aagcccggac gactgttcgg gcttgtcttt ttatatgttg gaaaatcagt
     8821 ggcaatgcaa tggcccaaca gaaaataaaa tctgtgacaa taaaggcata taacccgcgc
     8881 agaataacgt atagaaaatt aaaagtataa tttttatttt gtatgtatta tcttcgatac
     8941 attcaccaga cttattatca tttcagaaga attatttgat taaggtttta cttaaggcgt
     9001 aacaaatgat atttatcggc taactgaact tctcctttag gatgttttca ctccccggaa
     9061 tggggataaa aggcaaataa aataactaaa ggatttattc aatgaaaagc aaagtactgg
     9121 cacttttaat tcctgccctg ctcgccgcag gtgctgcaca tgcagccgaa gtttataata
     9181 aagacggcaa caaattagat ctgtatggca aagttgatgg cctgcattat ttttctgata
     9241 attcagcgaa agatggcgat cagagctatg cgcgtctggg ttttaaaggc gaaacccaaa
     9301 ttaacgatca actcactggc tacggtcaat gggaatacaa tattcaggca aacaacactg
     9361 aatcttcaaa aaaccagtca tggacccgtc tggcatttgc cgggctgaaa tttgcagatt
     9421 acggttcttt cgattacgga cgtaattatg gcgtaatgta cgacatcgaa ggctggaccg
     9481 atatgctgcc tgaatttggc ggtgactctt ataccaatgc agacaacttt atgactggtc
     9541 gtgccaacgg cgtcgcgact tatcgtaata ctgatttctt cggtctggta aatggtctga
     9601 acttcgcggt gcagtatcaa ggtaacaacg aaggtgccag taatggtcag gaaggcacca
     9661 acaacggacg tgatgttcgc catgaaaatg gtgacggctg gggtctttcc acaacatatg
     9721 atttaggcat gggctttagc gctggtgcgg catacacctc atctgaccgc accaatgacc
     9781 aggttaacca tactgcggcg ggtggtgata aagcagacgc gtggactgct gggctaaaat
     9841 acgatgctaa caatatttac ctggcaacca tgtattcaga aacgcgtaat atgaccccgt
     9901 ttggcgacag cgattatgct gttgcaaaca aaacccagaa ctttgaagtc actgcacagt
     9961 accagtttga ttttggtctg cgtccggcag tctctttcct gatgtctaaa ggccgtgacc
    10021 tgcacgctgc cgatggtgca gacaacccgg caggtgttga tgataaagat cttgttaaat
    10081 acgccgatgt tggcgcgact tactatttca ataaaaacat gtccacttat gttgactata
    10141 aaatcaacct gttggatgaa gatgacagtt tctacgctgc caacggcatc tctaccgatg
    10201 atattgtcgc tttaggtctg gtttatcagt tctaaatcct cctgcccgct gttatggcgg
    10261 gctttttctg cttattcttc ctcctttgat ctaaattaaa aatgtgaact ccgtcattac
    10321 acaaaaagtg tcatctggcg ttacacttta tgcggatact aaaacaggag gttttatgaa
    10381 cagaacgatt cttgtcccta tcgatatttc cgattcagaa ttaactcaac gcgtgattag
    10441 ccacgttgag gcagaggcaa agattgacga tgcagaggtt catttcctga cggtaatacc
    10501 ttcactgcct tactatgcct ctctgggttt agcgtattcc gcagaattac cggcaatgga
    10561 tgacctgaaa gcggaagcca aatcgcaact ggaagagatc attaaaaaat ttaaactgcc
    10621 aaccgacaga gtgcatgtcc atgttgagga aggctcgccc aaagaccgca ttctggaatt
    10681 ggcgaagaag atccccgctc atatgatcat cattgcttcc catcgaccgg atatcaccac
    10741 ttatctgctc ggttccaacg ccgcagctgt agtgcgtcac gcagagtgct ccgtgctggt
    10801 tgtgcgctga cactaacgcc cgcacattgc tgcgggcttt ttgattcatt tcgcaaatgt
    10861 gctgacattt tcccctctaa tccgtaccat acacgccaca gttttatatc agatttctta
    10921 tgctgggcgt tccggcatga cgcatactct tctgatgcca tataacgaat tgagtcgctt
    10981 ttaaatgtcg caaaatcaag aaattagtaa gaaagaacaa tacaacctga acaagtaagg
    11041 gcaaaaatca caactatctg acccataagt attttattta tttttcaata tgttaaacat
    11101 tatcagcgac aacaataagc tacgataacc cactcttttt ttgccccttt tttgcccctt
    11161 ctacagcgtt ttgccccatt tttgccatca acaattttcc tcataaagtc atttcaaagc
    11221 ctaatagctg ctctactttt ttcctcaata tcatgtcgcc agcaggaaac aacctaatcc
    11281 ctgttttagg cctcaaagct gttaatgata atcattttca ttttgtatgg gttatgctat
    11341 aaaacatagc cgttgctatg tttgttctaa ttttgctcac tataggtact aaattatgca
    11401 ctcaataaaa aaagtaacca tgctcttggg gtgcctcgcc ctcacctgct cgatcgcatt
    11461 tcaggcaagt gcaactgaaa aattcaaggt cattacaaca tttaccatca tcgcggatat
    11521 ggccaaaaac gtggctggag atgctgcaga agtctcatcc ataaccaagc ctggtgcaga
    11581 aattcatgag tatcagccta cccctggcga tattaaacgt gcgcaggggg cacaactgat
    11641 tctcgccaat ggtatgaatc tggaattgtg gttccaacgc ttttaccagc atctcaatgg
    11701 ggttccagaa gtaattgtct cttcgggtgt gacgccagta gggatcaccg aaggacccta
    11761 tgagggcaaa cctaatcccc atgcatggat gtcgccagat aatgctctga tttacgtcga
    11821 taatattcgt gatgcgttga taaaatacga tccggcaaat gcacaaacct accaacgtaa
    11881 tgccgatact tataaagcca agattaccca aacccttgcc cccctgcgta agcagattac
    11941 ggaactccct gagaatcagc gatggatggt caccagtgaa ggggcttttt cttatctcgc
    12001 acgcgatttg gggctaaaag agctttatct gtggccgatt aatgccgatc aacaaggaac
    12061 accgcagcag gtacgtaagg ttgttgatat agttaagaaa aatcatatcc cggcagtctt
    12121 tagcgagagt acgatttccg ataaaccagc gcgtcaggtt gcgcgtgaaa ccggcgcaca
    12181 ctacggcggt gtgctctatg tcgattccct gagcacagaa aacggcccgg tacctacgta
    12241 tatcgacctt cttaaggtta ctaccagtac gctggtacag ggaattaaag ccgggaaaag
    12301 ggagaaataa tgatgcaatc tgcaggcatt gtcgttaatg atgttactgt cacctggcgt
    12361 aacgggcaca cagcactgcg cgatgcatcc ttcacagtac caagtggatc tattgccgct
    12421 ctggttgggg taaacggttc cggaaaatcg acgttgttta aagcgattat gggatttgtg
    12481 cgtctgacca gcggaaagat atctgttctg ggtattccca cgcgacaggc gctacagaaa
    12541 aacctggttg cctacgtccc tcagtcagaa gaggttgact ggtcatttcc tgtactggtg
    12601 gaagatgtgg tgatgatggg ccgctatggg catatgggta tgttgcgtat agccaaaaag
    12661 agggatcgtc agattgtcac cgatgcgctg gaacgtgtcg atatggtgga ttttcgccat
    12721 cgtcaaatcg gcgagctttc cggtggacaa aagaaacgcg tctttctggc gagagcgatt
    12781 gcacagcagg gtgatgtgat cctgctggat gaacctttta ccggcgttga tgtgaaaacc
    12841 gaagcgaaaa tcatcagcct gttgagggag ttacgcgcgg aaggcaaaac gatgcttgtc
    12901 tcaacgcaca atctcggctc agtcacgaca ttttgcgatt atacggtcat ggtcaaaggc
    12961 accgtcctgg caagcgggcc gacagacacc acttttacag ccgagaacct ggagctggct
    13021 tttagcggcg tactgcgcca tgtcacactt aacgactcgg aagaaagcat tattactgac
    13081 gatgaacgcc cttttgtggc acatcgaccg tcagcggtgc agagggaagc aagatgaacg
    13141 tgctactgga acccttcagc tatgaatata tgctcaatgc gatgtgggtc tcggcaatgg
    13201 tcggcggtct ctgcgctttc ctgtcatgct atctgatgct aaaaggctgg tcgctgattg
    13261 gcgatgcgct gtcgcactct attgtcccgg gtgttgcggg ggcatatatg ctggggcttc
    13321 cgttttctct gggagccttc ttttccggcg gactggcagc gggcagcatg ctgtttctta
    13381 accaacgcac ccgcttaaaa gaagatgcca tcatcggcct gatcttctcg tccttttttg
    13441 gcctggggct gtttatggtg tcattgaacc cgacatccgt gaacattcag accatcgtcc
    13501 tgggcaatat tctggcaatc gatccggcgg atatcctgca actcactatc attggcattc
    13561 tctcgatcat cgtgcttttt ttcaaatgga aagatcttat ggtgaccttt ttcgacgaga
    13621 atcacgcccg tgctattgga ttacatccgg gcagattaaa aatcctcttc ttcaccctgc
    13681 tgtcggtttc gaccgttgcg gcgctgcaaa cggttggtgc ctttctggtg atctgtctgg
    13741 tggtgactcc cggtgcaaca gcatggttgt taaccgatcg ttttccccgc ctgctcataa
    13801 ttgctgtaac tattggcagt gtaaccagtt tcctcggcgc gtgggtcagt tactttctgg
    13861 acggcgcgac aggtggaatc atcgtggtgg cgcagacact gctgtttctt ttagcatttg
    13921 tatttgcgcc cacccatgga cttttggcaa atcgtcggcg tgcgcataaa gcgctggagg
    13981 atcgctcatg atggcattat tactggagcc actgcagttc acctttatga gtcacgcact
    14041 gctgatttca ctggtagttt ccataccctg tgcgctgctg tcggtctttc tggtcctgaa
    14101 aggttgggca ctgatgggtg acgcgatgag tcatgcggtt tttcctggca tcgtgcttac
    14161 ctggattctg gggttacctc tggctacagg ggcattcgtc gccggagtat tctgcgcggt
    14221 ggcgacggga tacctgaaag acaacagccg aatcaagcaa gatacagtta tggggattgt
    14281 tttttctggc atgtttgccg caggtctgat cttgtatatt gcaatcaaac cagacgtaca
    14341 tcttgaccat attcttttcg gcgacatgct cgggataacc atcggcgata taatccagac
    14401 ggtgattatt gccgggctgg ttacacttgt tattagcgca aaatggcggg attttttgct
    14461 gttcagcttt gattatcaac aggcgcaggt aagcggtttg catacgagat ggctgcatta
    14521 tgggctgctg tgcatggttt ccctgaccat tgtggcgacg ctgaaagcag taggtatcat
    14581 tctctctatt tctctgctca ttgcgcctgg cgccattgca gtactcctga cacaacgctt
    14641 tcatattgcg ctcctactgg cgactggcat atcggtaata gtgtcaatga ctggtgtctg
    14701 gctgtccttt tttatcgaca gcgcaccagc tccgacgatt gtcgtcttat tcgcggtcgt
    14761 gttcatcatg acgtttaccg ttaccagcat caacgcacgc acaaagggaa acgcccatac
    14821 acaggatctg ttatcaccca attagcacaa catccgagga cagcatcgtc cgccagccta
    14881 cagaagtccg gaaagccatg gaaggtcgtt gggaggaggt acgaattcta tcatgcaaaa
    14941 aatacgtaaa atcgataacg cctagaaatc attcaatact cgcactatcg aaagtttacc
    15001 agccaaccgc ggcacgttcc tgcatacgac gtgccgcagc ttctgttatt ccacgataaa
    15061 attcctaacc gaattcaaat ccttcagact attcagctct tccttcagct cccgctgacg
    15121 gcgataaatc tcgtcattgc gctcaacctg tgcctgagcc attgctgccg ccagttcttc
    15181 cagttccggc atcgactgtt tcacctgctg attatcggca tcaccccacg tcatcacgtc
    15241 ccgtacaatg tcggatttcg cagccatta
//
LOCUS       DAJVRF010000096        15236 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJVRF010000096 DAJVRF010000000
VERSION     DAJVRF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15236
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.096"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..749
                     /locus_tag="OQN13_002748"
     CDS             <1..749
                     /locus_tag="OQN13_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCS1616060.1"
                     /translation="SKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYEL
                     DKLTKGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEV
                     NAALFKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLLN
                     FDVKLPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASL
                     QLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(790..1722)
                     /locus_tag="OQN13_002749"
     CDS             complement(790..1722)
                     /locus_tag="OQN13_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS1616061.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            2086..4872
                     /gene="polA"
                     /locus_tag="OQN13_002750"
     CDS             2086..4872
                     /gene="polA"
                     /locus_tag="OQN13_002750"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCS1616062.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(5254..5886)
                     /locus_tag="OQN13_002751"
     CDS             complement(5254..5886)
                     /locus_tag="OQN13_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCS1616063.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6468..6977
                     /gene="yihI"
                     /locus_tag="OQN13_002752"
     CDS             6468..6977
                     /gene="yihI"
                     /locus_tag="OQN13_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCS1616064.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            7166..8539
                     /gene="hemN"
                     /locus_tag="OQN13_002753"
     CDS             7166..8539
                     /gene="hemN"
                     /locus_tag="OQN13_002753"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCS1616065.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8729..8839)
                     /locus_tag="OQN13_002754"
     CDS             complement(8729..8839)
                     /locus_tag="OQN13_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCS1616066.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8951..10360)
                     /gene="glnG"
                     /locus_tag="OQN13_002755"
     CDS             complement(8951..10360)
                     /gene="glnG"
                     /locus_tag="OQN13_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCS1616067.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10372..11421)
                     /gene="glnL"
                     /locus_tag="OQN13_002756"
     CDS             complement(10372..11421)
                     /gene="glnL"
                     /locus_tag="OQN13_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCS1616068.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11595..13004)
                     /gene="glnA"
                     /locus_tag="OQN13_002757"
     CDS             complement(11595..13004)
                     /gene="glnA"
                     /locus_tag="OQN13_002757"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCS1616069.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13377..15200
                     /gene="typA"
                     /locus_tag="OQN13_002758"
     CDS             13377..15200
                     /gene="typA"
                     /locus_tag="OQN13_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCS1616070.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         3965 a         3803 c         3915 g         3553 t
ORIGIN      
        1 agtcgaaatt taatcagatt aacattatca atcacgggga agacgttgcc caaatcgatg
       61 ctttcgttgc aaaaaccatg ctggatcgcg ttaaagacaa agattatatc aatgtcaatc
      121 tgacctacga acttgataag ttaacaaaag ggaatcagca actcggtagt ggtgagtggt
      181 cattgattgc tgaatctatt gatccctcag cagtgcgcca atttatcatc cagtataaca
      241 ttgctatgca gaagcagctt gctgtacacc ctgagttagc aaacgatgaa gttgctctgc
      301 aagaagtgaa tgctgcattg ttcaaagagt atttaccgtt attacaaaaa agtgagccga
      361 ccattaaaca accggtaaga tggaagaacg cactcggcga actaaatgcc aatctggata
      421 tcagtattgc cgacccagcc aaatcttcat catccacaaa caaagatatc aaattgctca
      481 attttgatgt gaagttaccg cttaatgtcg tcacagaaac cgcaaaacag cttaatttat
      541 ctgaaggaat ggatgcggaa aaagcgcaaa agcaggctga taaacaaatc agcgggatga
      601 tgacattggg tcagatgttt cagttaatca cgattgacaa caataccgcc tcgctgcagc
      661 tgcgttatac accgggtaaa gttgtgttta acggacagga gatgagcgaa gaagaattta
      721 tgtctcgtgc cggacgtttt gttcattaaa atactcactc gcctctgctc attcagaggc
      781 gagtaaaact tacttattct gacgctgtga tgacatgaga gacgtcagaa gccggtcttt
      841 ttcctgccac aatgagttca gccattgctg aaaatggcgc ttaaagcttt tgtcattgat
      901 gtagtcgcca tgtaactcat cggcaatagg ctgtaaatcc acatgtacga caatccgcgt
      961 cagtttaccg cttaacatat cgaagaacgg ctgacggtta ttgtccggat aacacagcgt
     1021 aacattaagc agtttatcga attgtttacc cagtacattt agcgccatcg caatgcctgc
     1081 cgcctttggt ggcaacaagt tttgaaaagt ggagtgggtt tgctgatgtt tttcttgcgt
     1141 gaaacgggag ccttcaacga aattgacaat ggtggtggga tgcaggcgaa acttttcaca
     1201 agaacgccga gtggtttcaa catctttacc acgtcgctcc ggatgacgta ataaataagc
     1261 gcgggaataa cgcttcataa acggcatatc cagcgcccag cacgccaggc caagaaatgg
     1321 cacccaggcc agctgttgct tgaggaaata tttattcatc ggaatgtgct tacgaaacag
     1381 cacgcacagt acgacaatat ctgcccaact acggtggtta caaataagca gataccagtt
     1441 cttcttactt aacccttcca gcccgtgaac ttcccattgc aggtgtgggt tcagatgcag
     1501 taataccgcc agaccttcac accagcaata catcataaaa tcacaaaaac gcgaaacctt
     1561 tcgccaaatg actggcacag gtagcaatag ttttacaatc ccggcaatga tgatcgggac
     1621 ggaacaaaaa atagtgacca aaatggtcaa tacgatactc agcaaaaggg ttatcgcagc
     1681 gagtattctc gtcataatga atttattcaa aaggttagcc atagacagtg cgctaaagaa
     1741 gcgcaaggcg cagattttag cagaaaacga cccaaataac ggatgatcct taaggagaaa
     1801 aataatccat atctatccac attagaaaaa atcccattat ctcaattatt agggatggct
     1861 ttatttttaa ctgcatgaaa aacaaagaca aacatcatgc tgtaaaaagc atgataataa
     1921 attaaaagcg atgtaaataa tttatgcaca aagttatcca catgacgatt tgcgagcgat
     1981 ccagaagatc tacaaaagat tttcacgaaa agcggtgaaa aactcatgtt ttcatcctgt
     2041 ctgtggcatc ctttacccat aatctgataa acaggcacgg acattatggt tcagatcccc
     2101 caaaatccac ttatccttgt agatggttca tcttatcttt atcgcgcata tcacgcgttt
     2161 cccccgctga ctaacagcgc aggcgagccg accggtgcga tgtatggtgt cctcaacatg
     2221 ctgcgcagtc tgatcatgca atataaaccg acgcatgcag cggtggtctt tgacgccaag
     2281 ggaaaaacct ttcgtgatga actgtttgaa cattacaaat cacatcgccc gccaatgccg
     2341 gacgatctgc gagcacaaat cgaacccctg cacgcgatgg ttaaagcgat gggactgccg
     2401 ctgctggcgg tttctggcgt agaagcggac gacgttatcg gtactctggc gcgcgaagcc
     2461 gaaaaagccg ggcgtccggt gctgatcagc actggcgata aagatatggc gcagctggtg
     2521 acgccaaata ttacgcttat caacaccatg acgaatacca tcctcggacc ggaagaggtg
     2581 gtgaataagt acggcgtgcc gccagaactg atcatcgatt tcctggcgct gatgggtgac
     2641 tcctccgata acattcctgg cgtaccgggc gtcggtgaaa aaaccgcgca ggcattgctg
     2701 caaggtcttg gcgggctgga tacgctgtat gccgagccag aaaaaattgc tgggttgagc
     2761 ttccgtggcg cgaaaacaat ggcagcgaag ctcgagcaaa acaaagaagt tgcttatctc
     2821 tcataccagc tggcgacgat taaaaccgac gttgaactgg agctgacctg tgaacaactg
     2881 gaagtgcagc aaccggcagc ggaagagttg ttggggctgt tcaaaaagta tgagttcaaa
     2941 cgctggactg ctgatgtcga agcgggcaaa tggttacagg ccaaaggggc aaaaccagcc
     3001 gcgaagccgc aggaaaccag tgttgcagac gaagcgccag aagtgacggc aacggtgatt
     3061 tcttatgaca actacgtcac catccttgat gaagaaacac tgaaagcgtg gattgcgaag
     3121 ctggaaaaag cgccggtatt tgcatttgat accgaaaccg acagccttga taacatctct
     3181 gctaacctgg tcgggctttc ttttgctatc gagccaggcg tagcggcata tattccggtt
     3241 gctcatgatt atcttgatgc gcccgatcaa atctctcgcg agcgtgcact cgagttgcta
     3301 aaaccgctgc tggaagatga aaaggcgctg aaggtcgggc aaaacctgaa atacgatcgc
     3361 ggtattctgg cgaattacgg cattgagctg cgtgggattg cgtttgatac catgctggag
     3421 tcctacattc tcaatagcgt tgccgggcgt cacgatatgg acagcctcgc ggaacgttgg
     3481 ttgaagcaca aaactatcac ttttgaagag attgcgggta aaggcaaaaa tcaactgacc
     3541 tttaaccaga ttgccctcga agaggccgga cgttacgccg ccgaagatgc ggatgtcacc
     3601 ttgcagttgc atctgaaaat gtggccagat ctgcaaaaac acaaagggcc gttgaacgtc
     3661 ttcgagaata tcgaaatgcc gctggtgccg gtgctttcac gcattgaacg taacggtgtg
     3721 aagatcgatc cgaaagtgct gcacaatcat tctgaagagc tcacccttcg tctggctgag
     3781 ctggaaaaga aagcgcatga aattgcaggt gaggaattta acctttcttc caccaagcag
     3841 ttacaaacca ttctgtttga aaaacagggc attaaaccgc tgaagaaaac gccgggtggc
     3901 gcgccgtcaa cgtcggaaga ggtactggaa gaactggcgc tggactatcc gttgccaaaa
     3961 gtgattctgg agtatcgtgg tctggcgaag ctgaaatcga cctacaccga caagctgccg
     4021 ctgatgatca acccgaaaac cgggcgtgtg catacctctt atcaccaggc agtaactgca
     4081 acgggacgtt tatcgtcaac cgatcctaac ctgcaaaaca ttccggtgcg taacgaagaa
     4141 ggtcgtcgta tccgccaggc gtttattgcg ccagaggatt atgtgattgt ctcagcggac
     4201 tactcgcaga ttgaactgcg cattatggcg catctttcgc gtgacaaagg cttgctgacc
     4261 gcattcgcgg aaggaaaaga tatccaccgg gcaacggcgg cagaagtgtt tggtttgcca
     4321 ctggaaaccg tcaccagcga gcaacgccgt agcgcgaaag cgatcaactt tggtctgatt
     4381 tatggcatga gtgctttcgg tctggcgcgg caattgaaca ttccacgtaa agaagcgcag
     4441 aagtacatgg acctttactt cgaacgctac cctggcgtgc tggagtatat ggaacgcacc
     4501 cgtgctcagg cgaaagagca gggctacgtt gaaacgctgg acggacgccg tctgtatctg
     4561 ccggatatca aatccagcaa tggtgctcgt cgtgcagcgg ctgaacgtgc agccattaac
     4621 gcgccaatgc agggaaccgc cgccgacatt atcaaacggg cgatgattgc cgttgatgcg
     4681 tggttacagg ctgagcaacc gcgtgtacgt atgatcatgc aggtacacga tgaactggta
     4741 tttgaagttc ataaagatga tgttgatgcc gtcgcgaagc agattcatca actgatggaa
     4801 aactgtaccc gtctggatgt gccgttgctg gtggaagtgg ggagtggcga aaactgggat
     4861 caggcgcact aagattcgcc tgaacatgcc cttttttcgt aagtaagcaa cataagctgt
     4921 cacgttttgt gatggctatt agaaattcct atgcaacaac tgaaaaaaaa ttacaaaaag
     4981 tgctttctga actgaacaaa aaagagtaaa gttagtcgcg tagggtacag aggtaagatg
     5041 ttctatcttt cagacctttt acttcacgta atcggatttg gctgaatatt ttagccgccc
     5101 cagtcagtaa tgactggggc gttttttatt gggcgaaaga aaagatccgt aatgcctgat
     5161 gcgctatgtt tatcaggcca acggtagaat tgtaatctat tgaatttacg ggccggatac
     5221 gccacatccg gcacaagcat taaggcaaga aaattattcg ccgtcctgcg tttcttctac
     5281 aggctgcatc tcgctaaacc aggtatccag tttctgccgc agcttgtcca cgccttgttt
     5341 cttcaacgaa gaaaacgttt caacctgcac atcaccgtta aacgccagta cagcttcacg
     5401 caccatattc aattgcgctt tacgtgcgcc gcttgccagt ttgtccgctt tggtcagcag
     5461 caccagaacg gcgatattgc tgtctaccgc ccactcaatc atctgctgat ccaaatcttt
     5521 cagcggatgg cgaatatcca ttagcaccac cagaccttgc aggctctgac gtttttcgag
     5581 gtattcgccg agcgcacgct gccatttgcg cttcatctct tccgggactt ccgcataacc
     5641 gtacccaggc aagtcaacca gacgcttgcc gtcagccact tcaaacaggt tgataagctg
     5701 ggtgcgccct ggggtttttg aggtacgagc caggcttttc tggttagtca gcgtgttcag
     5761 cgcgctggat ttacctgcgt tggaacggcc tgcaaaagcc acttcaattc cggtatcgga
     5821 aggtaggtgg cgaatatcag gcgcactcat cacaaaatgc gtctgttgat aattcaaatt
     5881 agtcaaagcg gtcgtctccg tcagtcaaag ctttgtggcg attatacctg aaccataata
     5941 aaaggcggat ttttcggcgt gagcgttgta agtaaaagcc atacgctttg tgagacattg
     6001 ccgatagtct ttatgcgaaa tagcagagaa aattctgcga tgcatgtcaa ataagctata
     6061 taaatcagtg aattgctttt atataaaact ctgaaaatgt gcaaatactg atggcggttg
     6121 attgtttgtt taaagcaaag gcgtaaagta gcacccatag agcgaggacg ctaacaggaa
     6181 caatgactca ggatgagggt caggagcgcc aggaggcgaa gacagaggat tgtcaggaag
     6241 acaaacgtcc ggagacgtaa ttaaacggaa atggaatcaa cacggattgt tccggctaaa
     6301 ggaaaaacag ggtgtgttgg cggcctgcaa ggattgtaag acccgttaag ggttatgagt
     6361 caggaaaaaa ggcgacagag taatctgtcg ccttttttct ttgcttgctt tctgttagat
     6421 tccgccgcaa atctatactg aataaaacgg ctaaaagacg aaccattatg aaaccatcat
     6481 cttcaaactc acgcagcaaa ggtcatgcaa aagcgcgtcg aaaaacacgc gaggagctgg
     6541 atcaggaagc tcgtgatcgc aagcgtcaga aaaaacgtcg tggtcatgcg ccgggcagcc
     6601 gcgcagcggg cggaaacacc acgtcaggca gcaaaggcca gaacgcacca aaagatccac
     6661 gtattggcag taaaacccct attccattgg gcgtggctga aaaagtcacc aaacagcaca
     6721 aaccgaagag tgagaaacct atgctttcac cgcaggcgga gttggaatta ctggaaacgg
     6781 atgagcgtct ggatgcgctg ctggaacgtc tggaagcagg cgaaaccctg agcgccgaag
     6841 agcaatcctg ggtggatgct aaactggatc gtattgatga gttgatgcag aaactcggcc
     6901 tctcttatga cgatgacgaa gaagaggaag aagacgagaa gcaagaagac atgatgcgtc
     6961 tgctgcgggg taactaacgg attgccaccg tgggccttcc cgttctgctg ataacccttc
     7021 cggttatatg ttatctggtg tggttattcg ttaaactaca gcggttgtcg cggcgacaaa
     7081 agtggctgcg caaccggctg atgacccgaa acgggcatcc gccggtacgc cgtagccgcc
     7141 agagacgcca tcggaaggag tgagcatgtc tgtacagcaa atcgactggg atctggccct
     7201 gatccagaaa tataactatt ccgggccacg atacacctcg tacccgaccg cgctggagtt
     7261 ttcagaagac ttcggcgaac aggcgttttt acaagccgtg gcgcgctatc ctgagcgtcc
     7321 attatctctc tacgtacata tcccgttctg ccataagctt tgttacttct gcggttgcaa
     7381 taagattgtt actcgccagc agcacaaggc cgatcagtat ctggacgcgc tggagcaaga
     7441 aatcgtccat cgtgcaccgc tgttcgccgg gcgtcacgtc agccaattgc actggggcgg
     7501 cggaacgccg acgtatctga ataaagcgca aatcagccgc ctgatgaagc tgctgcgcga
     7561 aaacttccag ttcaatgccg atgcggagat ttcgatcgaa gtcgatccgc gggaaatcga
     7621 actggatgta ctcgatcatt tacgcgccga aggctttaat cgcctgagca tgggcgtgca
     7681 ggacttcaac aaagaagtgc aacgtctggt taaccgcgag caggatgaag agttcatctt
     7741 tgcactgctt aaccatgcgc gtgagattgg ttttacctcc accaacatcg acctgattta
     7801 cggcctgccg aaacagacgc cggagagttt cgcctttacc ctgaaacgtg tggcggagct
     7861 gaaccccgat cgtctgagtg tctttaacta cgcgcatctg ccgaccattt ttgctgctca
     7921 gcgcaaaatc aaagatgctg acctgccgag tccgcagcaa aaactcgata tcctgcagga
     7981 aaccatcgcc ttcctgacgc aatcgggcta tcagtttatc ggtatggatc actttgcccg
     8041 tccggatgac gagctggcgg tggcccagcg tgaaggcgtg ctgcatcgta acttccaggg
     8101 ctacaccact cagggcgata ccgatctgct ggggatgggc gtttccgcca tcagcatgat
     8161 tggcgactgc tacgcgcaga accagaaaga gttgaagcag tactatcagc aagtggatga
     8221 acaaggcaat gctctgtggc gtggtattgc gctaacgcgt gatgactgta ttcgccgcga
     8281 tgtgattaag tcgctcatct gcaacttccg tctggattac gcccctattg agaaacagtg
     8341 ggatttgcac ttcgctgatt actttgcgga agatctcaag ctgctcgccc cgttagcaaa
     8401 agatgggctg gtggatgtgg atgagaaggg aatacaggtg acggcgaaag gtcgcttgct
     8461 gatccgcaac atttgcatgt gctttgatac ctatctgcgc cagaaagcgc ggatgcagca
     8521 gttctcacgg gtgatttaaa tagtgaatgg cgcttcgttt acaaagtaga aaacgaaagc
     8581 gccatcaatg tgctgagaac aagattgcct gatgcgctac gcttatcagg cctacaaaac
     8641 ctgttgaaaa tattgctttt gtaggccgga taaggcgttc acgccgcatc cggcatgaac
     8701 aaagagcact tcatcagcaa tctgaaactc aactaaacag cccaatcatt gcggcacaca
     8761 gcacggcagc aacggcagtt tgcggtgtgt ggctgtcaac tgcgccactc gataccagat
     8821 taattattga ttccagcata atgactctcc ccgttttccg ggcaagatca tactgaactt
     8881 atcggaacag taaagcgtaa aataccagca atttgcgctc aataatcaat ctttacacac
     8941 aagctgtgaa tcactccatc cccagctctt ttaacttacg cgtcagggtg ttgcggcccc
     9001 agccgagtag ccgcgccgct tcctgtttat gcccctgcgt atgtcgtaac gcggtcgtca
     9061 gtaacgtccg ctccagctct ggctgcgctt cggaaagcag attttgatga ccggaacgca
     9121 gcgctctgtc tgcccactgc gcaagaagcg ttgcccagct gtccggttgc atttgcgaag
     9181 tactctccgc aaccgttgat tcaaacagtt cgccgggcaa atcctgaatc aacacttcct
     9241 gcccggcggc catcaccgtt agccagcggc aggtgttttc cagctggcgc acgttgcctg
     9301 gccacgccag acgcgtcaga gcagcttcgg tttccggatg cagtaacttc gcttctacgc
     9361 ccagttcgcg cgcggcaacc tgtaaaaaat ggcgcgccag acggggaata tcttcccgac
     9421 gttcgcgcag cggcggcaga tgaacgcgga taacgttcag gcggtggaac agatcctcac
     9481 ggaacttacc ttcctgcact cgctgttcga gattctggtg agtggcagcg ataatacgca
     9541 catccacttt caccggcgca tagccgccaa cgcggtaaaa ctgaccgtct gccagcacgc
     9601 gcagcaaacg cgtctgcaca tccagcggca tatcaccaat ttcgtcgagg aataatgtac
     9661 cgccatcggc ctgttcaaaa cgcccctgac gaatggtatt cgcgccagta aacgcgcctt
     9721 tctcgtggcc aaacagttct gattcgatca aatcttttgg gatagctgcc atattcagcg
     9781 cgataaacgg cgctttggcg cgcggactgt ggcgatgcag ggcatgagcg accagttctt
     9841 taccggtgcc ggattcgccg ttaatcagca cgctaataga agaacgcgaa agccgaccga
     9901 taatacggaa cacgtcctgc atggctggcg cttcgccgat gatatcggtc gttgggccgt
     9961 taagctgaat attacgcggc tgctgctgtt cctggtaatg actgatagcg cgctcaacca
    10021 gcgccactgc ttcgtcgata tcaaacggtt tgggcagata atcaaacgcc ccttgttgat
    10081 aggcgctgac ggcagcatcc agatcggaat gtgcggtcat aatgatgacc ggaagcattg
    10141 gatggcgctg tttaatctgc ttgagcagcg ccagcccgtc cattcccggc atacggatat
    10201 ctgaaagcag cacatccggc gttttgctcg ccagcgcctc cagtacttcc gcgccgttct
    10261 caaatgtcgt acaggttaaa cccgctccag cgagcgcacg ttcaagcacc caacggatgg
    10321 aactatcgtc atcgactacc cagactatcc ctcgttgcat aaacgtcacc tttatttcct
    10381 gataggcagg taaaccgaga actcggtatg acctggccaa ctggtaaatt caattttgcc
    10441 tgaatgctga tcaatcaaat tacgggcgat ggataagcca agcccggtgc caccttcgcg
    10501 gccgctgacc atcgggtaaa acagcgtatc ctgcaaatga ggcggaatgc ccggcccgtt
    10561 atcttccaca tcaatccgcg ccgccagccg gtagcgctcg ccgtgtaagg tcagttgaaa
    10621 cgcggtgcgg gtacgcagaa tgatttcacc gccttccggc cccagcgcct gtagcgcatt
    10681 gcgcacaata ttcagcaaga cctgttcaat ttgatccggg tcgtgcgcca gttccggcag
    10741 gctggggtcg taatcacgaa tcaaccgcac gttgtccggc agttccatcg acaccagcgt
    10801 caccacgcgt tcagctactt tgtgaatact ttcggtaacg cgcgtaccgg gcagctgcgg
    10861 ccccaacaga cggtcgacca gatttcgcag ccggtccgcc tgttcgataa tcactttggt
    10921 atattcgagt agtgatgggt caggtaacgc tttgctgagc agctgcgccg cgccacgtaa
    10981 acctccaagc ggatttttaa tctcatgtgc caggccgcgc actaaatcac gggcagcaac
    11041 ctgctgggcg tgctgtagct gttcctgact taagcggcgc tggttatcca tcggagccat
    11101 ctccagcagg atcatgccgt ccggcatacg ctgggccgtc acagaaagga tatgcgagcg
    11161 cccgtcgatg accagcgtca cttcgttatc ggtaaaacct tgccccgcct ccagactttc
    11221 ttgcatcagc tcgatattta atgagaagta gctcaacagt tccggtaacg gtgtaccaaa
    11281 caatttgcgg gagctttggg cgagcagttg ttgcgcggcg gggttggcgt aatggatcgc
    11341 cagattgtca tcaattaaca aaatactgtt aatcagcgag ttgaggatct gcccagcatc
    11401 gggctgcgtg cctgttgcca taaagcagtc tcctgaacag gttgcaccat tttagtgcat
    11461 tatagctttt tacggataaa aagcgcgaag catcagagaa ttgacggaga aaaaagccca
    11521 tgcagagatg ggctgaaagt ttccacggca actaaatccc ggcgttgttg cgccgggtag
    11581 tacaacttca actattagac gctgtagtac agctcaaact ctaccggatg cggagtcata
    11641 cgcacgcggt catcttcttc gcgacgtaga gcgatgtacg catcaattgc ttcgtcagtg
    11701 aacacgccac cggctttcag gaactcgcgg tccagatcca gttcgttcag tgcttcttcc
    11761 agagagcctg caacctgtgg gatctctttc gcttcttctg gcggcaggtc atacaggttt
    11821 ttgtccatgg cttcgcccgg atggatcttg ttcttgatac catcaagacc ggccatcagc
    11881 agggcagcaa agcacaggta cgggttagct gccggatccg ggaaacgtac ttcgatacga
    11941 cgtgctttcg gagaagaaac caccggaata cggatagacg cagaacggtt acgcgcagag
    12001 taagccagca ttaccggtgc ttcatagccc gggaccagac gcttataaga gttggtggtc
    12061 gggtttgcca gggcgttaat cgctttagcg tgtttgatta cgccgccaat gtagtacagc
    12121 gcctgctcag acagacctgc gtatttgtcg cctgcgaaca ggttaacgcc gtttttagac
    12181 agagacatgt ggcagtgcat accggagccg ttatcaccga acatcggttt tggcataaag
    12241 gtcgcggttt taccgaagcg gtgcgctacg ttgtgcacaa catatttgta gatctgaatt
    12301 tcgtcagctt ttttggtcat agtattgaag cgggtagcca cttcgttctg accagcagtc
    12361 gctacttcgt ggtgatgggc ttcaaccacc agacccatct gttccatcac cagacacatt
    12421 tcagaacgaa tatcctgagc cgagtctacc ggtggaaccg ggaagtaacc gcctttcact
    12481 gccggacggt gacctttgtt accaccttcg tattgggtgg aggagttcca tgcgccttcg
    12541 atatcgtcga tagcaacgtg ggaaccggag atagatgatc cgaaacggat gtcatcgaac
    12601 aggaagaatt caggttctgg cccgaacagt acggtgtcgg caatgccagt ggaacgcagg
    12661 taatcttcgg cgcgcttcgc aatggagcgc gggtcacggt catagccttg cagggtgcca
    12721 ggttcaagga tgtcgcaacg gataatcagg gtggagtcgg cgaagaacgg gtcaatcact
    12781 gcggtggatg cgtctggcat cagcaccatg tcggactcgt taatgccttt ccagccgcca
    12841 atcgaggagc cgtcaaacat tttgccttct tcgaagaatt cagcattcac ctgatgagca
    12901 gggatagtga cgtgctgttc tttaccttta gtatcggtga agcgcaaatc aacaaacttc
    12961 acttcgtgct cgttcagcat cgtcagtacg tgttcagcgg acatacttta actctcctgg
    13021 attggtcatg gtcgtcgtgg taacgaaatc tgcaattatt ttggccgtgt cgccgtaaaa
    13081 aagataaagc gaaatctgtg ccaactttta aattgcccct aaaaggcgtt atcatgcgca
    13141 ccatcgtgca aaagggctgc accacgatgt gaatgttgca ccaatatagt gcttcaatgg
    13201 aaacattaag caccatgttg gtgcaatgac ctttggataa ccctttttat gctccgtgaa
    13261 agcgatcaca aagggactct gcaatacttg tttgcggagg atgtttgtga tcctgttttg
    13321 tagtgcgatt aatccgtgta caataacgcg ctatttctaa tgcctgaggc aaagttgtga
    13381 tcgaaaaatt gcgtaatatc gccatcatcg cgcacgtaga ccatggtaaa accaccctgg
    13441 tagacaagct gctccaacaa tccggtacgt tcgactctcg tgccgaaacc caagagcgcg
    13501 tgatggactc caacgatttg gagaaagagc gtgggattac catcctcgcg aaaaacaccg
    13561 ctatcaaatg gaatgattac cgtatcaaca tcgttgatac cccggggcac gccgacttcg
    13621 gtggtgaagt tgaacgtgta atgtccatgg tagactcagt gctgctggtg gttgacgcat
    13681 ttgacggccc gatgccgcaa acgcgcttcg taaccaaaaa agcgtttgct tacggcctga
    13741 agccgattgt tgttatcaac aaagttgacc gccctggcgc gcgtcctgat tgggttgtgg
    13801 atcaggtatt cgatctgttc gttaacctcg acgcgaccga cgagcagctg gacttcccga
    13861 tcgtttacgc ttctgcgctg aacggtatcg cgggtctgga ccacgaagat atggcggaag
    13921 acatgacccc gctgtaccag gcgattgttg accacgttcc tgcgccggac gttgaccttg
    13981 acggtccgtt ccagatgcag atttctcagc tcgattacaa cagctatgtt ggcgttatcg
    14041 gcattggccg catcaagcgc ggtaaagtga agccgaacca gcaggtcact atcatcgata
    14101 gcgaaggcaa aacccgcaac gcgaaagtcg gtaaagtgct gggccacctc ggtctggaac
    14161 gtatcgaaac cgatctggcg gaagctggcg atatcgtggc gatcacgggc cttggcgaac
    14221 tgaacatttc tgacaccgtt tgcgacacgc aaaacgttga agcgctgccg gcactctccg
    14281 ttgatgagcc gaccgtttct atgttcttct gcgttaacac ctcgccgttc tgcggtaaag
    14341 aaggtaagtt cgtaacgtct cgtcagatcc tggatcgtct gaacaaagaa ctggtacaca
    14401 acgttgcgct gcgcgtagaa gaaaccgaag acgccgatgc gttccgcgtt tctggtcgtg
    14461 gcgaactgca cctgtctgtt ctgatcgaaa acatgcgtcg tgaaggtttc gaactggcgg
    14521 tatcccgtcc aaaagtaatc ttccgtgaaa tcgacggtcg taaacaagag ccgtatgaaa
    14581 acgtgactct ggacgttgaa gaacagcatc agggttctgt aatgcaggcg ctgggcgaac
    14641 gtaaaggcga cctgaaaaac atgaatccag acggtaaagg ccgcgtacgt ctcgactacg
    14701 tgatcccaag ccgtggtctg attggcttcc gttctgagtt catgaccatg acttccggta
    14761 ctggtctgct gtactccacc ttcagccact acgacgacgt acgtccgggt gaagtgggtc
    14821 agcgtcagaa cggcgtactg atctctaacg gtcagggtaa agcggtcgcg ttcgcactgt
    14881 tcggtctgca ggaccgcggt aagctgttcc tcggtcacgg tgcagaagtt tacgaaggtc
    14941 agattatcgg tattcacagc cgctctaacg acctgactgt aaactgcctg accggtaaga
    15001 aactgaccaa catgcgtgct tccggtactg acgaagctgt tgttctggtt ccgcctatcc
    15061 gcatgactct ggaacaagct ctggagttca tcgatgatga cgaactggta gaagtgactc
    15121 cgacctctat ccgtattcgt aaacgtcacc tgacggaaaa cgatcgtcgt cgcgccaacc
    15181 gcgcaccgaa agacgattaa tttcgttttt tagttataaa aaacctgcca tcgctg
//
LOCUS       DAJVRF010000097        15230 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJVRF010000097 DAJVRF010000000
VERSION     DAJVRF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.097"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            107..277
                     /locus_tag="OQN13_002759"
     CDS             107..277
                     /locus_tag="OQN13_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1616071.1"
                     /translation="MKWEGDPPPFHEIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDK
                     YRDGRGREWKDI"
     gene            493..582
                     /locus_tag="OQN13_002760"
                     /pseudo
     CDS             493..582
                     /locus_tag="OQN13_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(583..1641)
                     /gene="modC"
                     /locus_tag="OQN13_002761"
     CDS             complement(583..1641)
                     /gene="modC"
                     /locus_tag="OQN13_002761"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCS1616072.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1644..2333)
                     /gene="modB"
                     /locus_tag="OQN13_002762"
     CDS             complement(1644..2333)
                     /gene="modB"
                     /locus_tag="OQN13_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCS1616073.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2333..3106)
                     /gene="modA"
                     /locus_tag="OQN13_002763"
     CDS             complement(2333..3106)
                     /gene="modA"
                     /locus_tag="OQN13_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1616074.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3272..3421)
                     /gene="acrZ"
                     /locus_tag="OQN13_002764"
     CDS             complement(3272..3421)
                     /gene="acrZ"
                     /locus_tag="OQN13_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCS1616075.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3550..4338
                     /gene="modE"
                     /locus_tag="OQN13_002765"
     CDS             3550..4338
                     /gene="modE"
                     /locus_tag="OQN13_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCS1616076.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4406..5878
                     /gene="modF"
                     /locus_tag="OQN13_002766"
     CDS             4406..5878
                     /gene="modF"
                     /locus_tag="OQN13_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCS1616077.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6139..7155
                     /gene="galE"
                     /locus_tag="OQN13_002767"
     CDS             6139..7155
                     /gene="galE"
                     /locus_tag="OQN13_002767"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCS1616078.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7165..8211
                     /gene="galT"
                     /locus_tag="OQN13_002768"
     CDS             7165..8211
                     /gene="galT"
                     /locus_tag="OQN13_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCS1616079.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8215..9363
                     /gene="galK"
                     /locus_tag="OQN13_002769"
     CDS             8215..9363
                     /gene="galK"
                     /locus_tag="OQN13_002769"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCS1616080.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9357..10397
                     /gene="galM"
                     /locus_tag="OQN13_002770"
     CDS             9357..10397
                     /gene="galM"
                     /locus_tag="OQN13_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCS1616081.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10600..11352
                     /gene="gpmA"
                     /locus_tag="OQN13_002771"
     CDS             10600..11352
                     /gene="gpmA"
                     /locus_tag="OQN13_002771"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCS1616082.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11511..12563)
                     /gene="aroG"
                     /locus_tag="OQN13_002772"
     CDS             complement(11511..12563)
                     /gene="aroG"
                     /locus_tag="OQN13_002772"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCS1616083.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            12879..13259
                     /locus_tag="OQN13_002773"
     CDS             12879..13259
                     /locus_tag="OQN13_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCS1616084.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13373..14314
                     /gene="zitB"
                     /locus_tag="OQN13_002774"
     CDS             13373..14314
                     /gene="zitB"
                     /locus_tag="OQN13_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCS1616085.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14311..15030)
                     /gene="pnuC"
                     /locus_tag="OQN13_002775"
     CDS             complement(14311..15030)
                     /gene="pnuC"
                     /locus_tag="OQN13_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCS1616086.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15068..>15230)
                     /gene="nadA"
                     /locus_tag="OQN13_002776"
     CDS             complement(15068..>15230)
                     /gene="nadA"
                     /locus_tag="OQN13_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCS1616087.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3862 a         4006 c         3805 g         3557 t
ORIGIN      
        1 actggataac ttgaaaaaaa taaacggtaa aaacgaaaag ttactcggag ggaagacagc
       61 caggacaatc gcagcacagt tcaggattgc ccgagacaca tccggattga aatgggaagg
      121 tgacccaccg ccatttcatg aaatccgaag tttatccggt cgcctgcact cagcagaaaa
      181 aggcggtgat ttcactcagg cgctccttgg acatcgttca tccagcatga cggataaata
      241 ccgtgacgga cgagggaggg aatggaaaga catttaaaat taatttcgaa tgatttcgaa
      301 acaccacttg taattaattg attataaatg attaattcct aatgggcatt tgcttcatga
      361 atgctccttt gcatttagca actgactgta acagctaaaa ttagtcgctt ttggcggtga
      421 atgctgaatt aaaacgcaga attttgtccg atagtggtat cggtgtacca ttcgcgcaga
      481 cgaaaattct taaatgacgc ggtaaaagcg cgcgccaaca ttgtgattgg tgataacacc
      541 accgacagca ttgcccagtt catttatagc cacctgattt aatcaggcgg ttatcgacac
      601 acttttaatt tgcgcgtaca gccacaggcc aggtttgatc gccagttcat ccctggccca
      661 cgggctgata cgcgcccaca gcgttttacc gccgacttcc agctccactt ccacctggcc
      721 gttgtcgtca taactattaa caacttttgc ccgcaatacg ttacgaatgc tggtttgctg
      781 cggcggttgt aaaaccagag aaacatcgga agcctgaatg cggatacgta gcgcagcttg
      841 cagcggttcg tccagcttat tgacccacaa atgctgatcg cccagcgcca gcgcggtcat
      901 cgcgtaatgc ggatgatgct caagtaccgt cactttcaga atgctacttt gttgctcttt
      961 cggcagccac ggattcatca cgctactgcc ccacacttcc tccagcgcgc caaaggcttt
     1021 cacctgaccg ttttccagta ccatcactct gtctgccaga tggaggatct catccagcga
     1081 atggctgaca tacaacatcg gaatgttgat ttcccgtgtc agccgttgca gataaggcaa
     1141 cagttcgcgt ttacgcggaa tatccagtga cgccagcggt tcatccagca acagcaattc
     1201 cggtgctgtc agcaaagccc gaccaatcgc cacgcgctgt ttttcgcctc cggacaggct
     1261 gcctggtaaa cggtcaagca acggttcaat gcctaaaagc gccaccagct tatcgaactg
     1321 atcgaccata cttttcgaca tgccgtagcg cagattgcca cgcactttgt aatgcgggaa
     1381 cagccgcgca tcctgaaaaa catagccaac gcgacgcttt tccggcgtca ggcagatacc
     1441 tttttcggca tcatttagta cccgcccatt gaggacaatc cgcccttttt gcgggcgcgt
     1501 cagtccactg atggcgttaa tcagcgaagt ttttccggca ccggagacgc caaagatagc
     1561 agtgatgcca ttggcgggca gtgtttcatt aatggtcagg caatggttgc ccaacgtctg
     1621 ggaaaaattc agctccagca tgattagcgc cccgcccgtt cacggctgat tctggccagc
     1681 cattctgaaa tcaacaggga gatcatcgcc agcgcaatag aaataatgca cagtctcgcc
     1741 gcgccacttt ccccgccggg ggtctggatc agggtataca tggcagaggg aatggttcgc
     1801 gtttcgccag gaatgttcga cacaaaggtg atggttgcac caaactcacc gagagaacga
     1861 gcaaaagcca gtaccgtacc aacaataatt cccggtaagg tcagcggtaa cgtgatagta
     1921 aagaaaacgc gccagcgccc ggcacctagt gttcttgcgg cctgttcgag tttgacatca
     1981 accccttcca gcgccagacg aattgcccgc accatcagcg gaaacgacat gacggcggca
     2041 gcgagaaccg cgccgcgcca gctaaaggcg aaggtaatac caaaccagtc atacagacgt
     2101 tcaccgataa atccgcgccg tcccatcgaa actaataata agtaaccgac gaccacgggc
     2161 ggtaacacca gcggtagatg cagtacgctg tcgagcagag ctttgcccgg aaacgtgcaa
     2221 cgcaccagta accaggcaaa aaagatccca aacggcaggc taaacagcac agccagggaa
     2281 gaaactttca ggcttaataa aactgcctgc cattctggat cggtcagtat cattacttgg
     2341 ttgtaaatcc gtaacgttta aagatttccg ctgcctgcgg tcccttcaga taatcataaa
     2401 atgctttcac tgtcgcattg ttatgccctt ccacaacagc aaccggatat tccacttttt
     2461 tatgtgaatc ttccgggaag gtggcaacca cttttacccc tttgctgaca actgcgtcag
     2521 aaccgtaaac aatgcccaac ggcgcttcat tgcgttcgac caacgccagc gcgccacgaa
     2581 catcttccgc cggagccagt ttcggggaga gcgtatccca tgcgcccagt ttttgcagtg
     2641 cttcttttgc ataaatgcca gcagggacat gttccggatc gccaaccgcg aggcgaccgc
     2701 cattcagcag cgaagtccag ttagttgtgc tgtcgatgat gaaatctttc tgctcgctgg
     2761 ctttcggtgc tacaacgacc aggctattgc cgagcagtgt ctggcgcgaa gccgtatcga
     2821 tcgctttttt atcaaccgca taatccatcc atttctgatc ggcagagata aacagatccg
     2881 caggcgctcc cgcttcaatc tgacgggcca gagtagatga cgaagcgaaa gaagaaacca
     2941 catccacgcc tttttctttt ttatactgcg tagcaatgtc ctgcattgcg ttagtcagtg
     3001 atgcggcggc gaacaccgtg attttccctt catctgccag tgtattgcca gcaacagcga
     3061 aagagagtgc cgccccggca aacaagttca accatttacg agccattggt aaccccttag
     3121 tgtaacgtta tataggtgac aatgtaacgg ctagctcaga attttcccag cagttattgc
     3181 taacctttgt gaggtagata agaaaaaata tcggctggaa aagcaggaag ttgagaaaaa
     3241 gaaatgcccg actaagcggg cattcaggga atcaatgatt ttgtccgggc tggtcttttt
     3301 taccaacacc agaaaagatg ttgaatactt caccaagacc gtaaatcaga cccaggatga
     3361 tggccatcac gacaggtacc atgattacgg cgaataccag actttttaat aactctaaca
     3421 tggtcaactc cagatatagt catgagacta ttctaaccgc taagcacaga aaagcactcc
     3481 ccttttgtgc ggtcagcttt gcgtggcgtt cgttttccgt cacaataaga cttttgccag
     3541 gacattgtta tgcaggccga aatccttctc acccttaagc tccaacaaaa attattcgcc
     3601 gacccgcgcc ggatttcgct actaaaacac attgcgcttt ccggttccat tagccaggga
     3661 gcgaaagatg ccggtatcag ctataaaagc gcctgggatg ccattaacga gatgaatcag
     3721 ttaagcgagc atattctggt tgagcgcgca acaggcggta aaggtggcgg cggcgcagta
     3781 ctgacccgct atggtcagcg actgattcag ctctatgact tactggcgca aatccagcaa
     3841 aaagcctttg atgtgttaag tgacgatgac gccctgccgc tgaacagcct gctggccgcg
     3901 atctcacgtt tttcactgca aaccagcgcc cgtaaccagt ggttcggtac catcaccgcc
     3961 cgcgatcatg atgacattca acagcatgtt gatgtcttac tggctgacgg aaaaacacgc
     4021 ctgaaagtcg caattaccgc acaaagcggc gcacgtctgg ggctggatga aggcaaagaa
     4081 gtgttgatat tgctaaaagc gccgtgggta ggcattactc aggacgaagc ggtcgcacaa
     4141 aacgccgaca accaattacc gggtattatt agtcatattg agcgcggcgc agagcagtgc
     4201 gaagtattaa tggcgctacc cgacgggcaa acactgtgcg ccacagtgcc ggtaaatgaa
     4261 gcgacttctc ttcagcaagg gcagaatgtc acggcctact ttaatgccga cagcgtgatt
     4321 atcgccacgc tgtgctaagc gtgttgacaa tttgttatga aacacgtatc cctgtcagta
     4381 atcgctgcac aaaatgggat ataaaatgtc atcgttgcaa attttgcaag gcacgtttcg
     4441 tcttagcgac acaaaaacgc tgcaattgcc tcagctaacg ttaaacgcgg gtgatagttg
     4501 ggcgtttgtc ggttcgaatg gaagcgggaa atcggccctg gcccgcgcgc tggcggggga
     4561 acttccgctt ttgaaaggtg aacggcaaag ccagttttcc cacatcactc gtctctcctt
     4621 cgagcaattg caaaaactcg tcagcgacga atggcagcgg aataacaccg atatgctcgg
     4681 ccctggcgaa gatgacaccg gacgcactac ggctgaaatc attcaggatg aagtaaagga
     4741 tgcaccgcgt tgcatgcaac tggcgcagca gttcggtatt accgccctcc tcgaccgacg
     4801 ctttaaatac ctttccactg gcgagacgcg aaaaaccctg ctgtgtcagg cgctgatgtc
     4861 ggagcctgac ttgttgattc ttgatgagtc gttcgatggc ctggatgttg cctcacgtca
     4921 gcagctggct gagcgactcg cctcgttaca tcagtccggt attactctgg tactggtgct
     4981 caatcgcttc gatgagatcc cggagtttgt ccagtttgct ggcgtgctgg cggattgcac
     5041 gttagcggaa actggcgcta aagaggaact gctccaacaa gcactcgtcg cgcaactggc
     5101 gcatagtgaa cagcttgaag gtgtgcaact gccggagccg gatgaacctt cagcacgtca
     5161 cgccttaccc gccaacgaac cgcgcattgt gctgaacaat ggcgtggttt cttataacga
     5221 tcgccccatt cttaataacc ttagctggca ggtgaatcca ggtgaacact ggcaaattgt
     5281 cgggccaaat ggtgcaggaa aatcgacgtt attaagcctg gttactggcg atcatccgca
     5341 aggttacagc aacgatttga cgcttttcgg acgacgtcgc ggcagcggcg aaaccatctg
     5401 ggatatcaaa aagcatatcg gttacgtcag cagtagtttg catctggatt accgggtcag
     5461 cactaccgtg cgtaatgtga ttctttctgg ctattttgat tcgattggca tttatcaggc
     5521 cgtttcggat cgccagcaaa aactggtgca gcagtggctg gatattctcg gcattgataa
     5581 acgtacggct gacgctccgt tccatagtct ttcctgggga cagcagcgtc tggcgctgat
     5641 cgtccgcgca ctggtgaaac atccgacgtt gcttattctc gatgaaccac tacaggggct
     5701 tgatccgctc aatcgccagc ttatccgccg ttttgttgat gtgctgatta gcgaaggtga
     5761 aacgcaattg ttatttgttt cgcaccacgc tgaagatgcg cctgcctgta ttacccatcg
     5821 ccttgagttc gtgccggacg gtggactcta tcgctatgtg ctgacaaaaa tatattgagt
     5881 cggtagtgct gaccttgccg gaggcggcct aagcgccctc tccggccaac ggttcgacgc
     5941 atgcaggcat gaaaccgctt cttttttcag ataaaaagcg caatcattca taaaccctct
     6001 gttttataat cacttaatcg cgcataaaaa acggctaaat tcttgtgtaa acgattccac
     6061 taatttattc catgtcacac ttttcgcatc tttgttatgc tatggttatt tcataccata
     6121 agcctaatgg agcgaattat gagagttctg gttaccggtg gtagcggtta cattggaagt
     6181 catacctgcg tgcaattact gcaaaacggt catgatgtca tcattcttga taacctctgt
     6241 aacagtaagc gcagcgtact gcctgttatc gagcgcttag gcggcaaaca tccaacgttt
     6301 gttgaaggcg atattcgtaa cgaagcgttg atgaccgaga tactgcacga tcacgctatc
     6361 gacaccgtga tccacttcgc cgggctgaaa gccgtcggcg aatcggtaca aaaaccgctg
     6421 gaatattacg acaacaatgt caacggcact ctgcgcctga ttagcgccat gcgcgccgct
     6481 aacgtcaaaa actttatttt tagctcctcc gccaccgttt atggcgatca gcccaaaatt
     6541 ccatacgttg aaagcttccc gaccggcaca ccgcaaagcc cttatggcaa aagcaaattg
     6601 atggtggaac agatcctcac cgacctgcaa aaagcccagc cggactggag cattgccctg
     6661 ctgcgctact tcaacccggt tggcgcgcat ccgtcgggcg atatgggcga agatccgcaa
     6721 ggcattccga ataacctgat gccatacatc gcccaggttg ctgtaggccg tcgcgactcg
     6781 ctggcgattt ttggtaacga ttatccgacc gaagatggta ctggcgtacg cgattacatc
     6841 cacgtaatgg atctggcgga cggtcacgtc gtggcgatgg aaaaactggc gaacaagcca
     6901 ggcgtacaca tctacaacct cggtgctggc gtaggcagca gcgtgctgga cgtggttaat
     6961 gccttcagca aagcctgcgg caaaccggtt aactatcatt ttgcaccgcg tcgcgaaggc
     7021 gaccttccgg cctactgggc ggacgccagc aaagccgacc gtgaactgaa ctggcgcgta
     7081 acgcgcacac tcgatgaaat ggcgcaggac acctggcact ggcagtcacg ccatccacag
     7141 ggatatcccg attaaggaac gaccatgacg caatttaatc ccgttgatca tccacatcgc
     7201 cgctacaacc cgctcaccgg gcaatggatt ctggtttcac cgcaccgcgc taagcgcccc
     7261 tggcaggggg cgcaggaaac gccagccaaa caggtgttac ctgcgcacga tccagattgc
     7321 ttcctctgcg caggtaatgt gcgggtgaca ggcgataaaa accccgatta caccgggact
     7381 tacgttttca ctaatgactt tgcggccttg atgtctgaca cgccagatgc gccagaaagc
     7441 aacgatcctt tgatgcgttg ccagagcgcg cgcggtacca gccgggtgat ctgcttttca
     7501 ccggatcaca gtaaaacgtt gccagaactg agcgttgcgg cattgacgga aatcgtcaaa
     7561 acctggcagg agcaaaccgc agagctggga aaaacgtacc cgtgggtgca ggtctttgaa
     7621 aacaaaggtg cggcgatggg ctgctctaac ccgcatccgc acggacagat ttgggcaaat
     7681 agcttcctgc ctaacgaagc tgagcgcgaa gaccgcctgc aaaaagaata ttttgccgaa
     7741 cagaaatcac caatgctggt ggattatgtt cagcgcgagc tggcagacgg tagccgtacc
     7801 gttgtcgaaa ccgaacactg gttagccgtt gtgccttact gggctgcctg gccgttcgaa
     7861 acgctactgc tgcccaaagc ccacgttttg cggatcaccg atttgaccga cgcccagcgc
     7921 agcgatttgg cgctggcgtt gaaaaagctg accagtcgtt atgacaacct cttccagtgc
     7981 tccttcccct actctatggg ctggcacggc gcaccattta atggcgaaga gaatcaacac
     8041 tggcagctgc acgcgcactt ttatccgcct ctgctgcgct ccgccaccgt acgtaaattt
     8101 atggttggtt atgaaatgct ggcagagacc cagcgagacc tgaccgcaga acaggcagca
     8161 gagcgtttgc gcgcagtcag cgatatccat tttcgcgaat ccggagtgta agaaatgagt
     8221 ctgaaagaaa aaacacaatc tctgtttgcc aacgcatttg gctaccctgc cactcacacc
     8281 attcaggcgc ctggccgcgt gaatttgatt ggtgaacaca ccgactacaa cgacggtttc
     8341 gttctgccct gcgcgattga ttatcaaacc gtgatcagct gtgcaccacg cgatgaccgt
     8401 aaagttcgcg tgatggcagc cgattatgaa aatcagctcg acgagttttc cctcaatgcg
     8461 cccattgtcg cgcatgaaaa ctatcaatgg gcgaactacg ttcgtggcgt ggtgaaacat
     8521 ctgcaactgc gtaacaacag cttcggcggt gtggacatgg tgatcagcgg caatgtgccg
     8581 cagggtgccg ggttaagttc ttccgcttca ctggaagtcg cggtcggaac cgtattgcag
     8641 cagctttatc atctgccgct ggacggcgca caaatcgcgc ttaacggtca ggaagcagaa
     8701 aaccagtttg ttggctgtaa ctgcgggatc atggatcagc taatttccgc actcggcaag
     8761 aaagatcatg ccttgctgat tgactgccgc tcactgggga ctaaagcagt ttccatgccg
     8821 aaaggtgtgg ctgtcgtcat catcaacagt aacttcaaac gtaccctggt tggcagcgaa
     8881 tacaacaccc gtcgtgaaca gtgcgaaacc ggtgcgcgtt tcttccagca gccagctctg
     8941 cgcgatgtca ccattgaaga gttcaacgct gttgcacatg agctggaccc aatcgtggcg
     9001 aaacgcgtgc gtcatatcct gactgaaaac gcccgcaccg ttgaagctgc cagcgcgctg
     9061 gagcaaggcg atctgaaacg tatgggcgag ttgatggcgg agtctcatgc ctctatgcgc
     9121 gatgatttcg aaatcaccgt gccgcaaatt gacactctgg tagaaatcgt caaagcagtg
     9181 attggcgaca aaggtggcgt acgcatgacc ggcggcggat ttggcggctg tatcgtcgcg
     9241 ctgatcccgg aagagctggt gcctgccgta cagcaagctg tcgctgaaca atatgaagca
     9301 aaaacaggta ttaaagagac tttttacgtt tgtaaaccat cacaaggagc aggacagtgc
     9361 tgaacgaaac tcccgcactg gcacccgatg gtcagccgta ccgactgtta actttgcgta
     9421 acaacgcagg gatggtagtc acgctgatgg actggggtgc gactttactt tcagctcgta
     9481 ttccgctttc cgatggcagc gtccgcgagg cgctgctcgg ctgtgccagc ccggaatgct
     9541 atcaggatca ggccgcgttt ctgggggcct ctattggtcg ttatgccaac cgcatcgcca
     9601 atagccgtta tacctttgac ggtgaaacgg tgacgctttc gccaagtcag ggcgttaacc
     9661 agctgcacgg cggcccggaa gggttcgaca aacgtcgctg gcagattgtg aaccagaacg
     9721 atcgtcaggt gctgtttgcc ctgagttcag atgatggcga tcagggcttc ccgggtaatc
     9781 tcggcgcgac ggtgcaatat cgtctgaccg acgataaccg tatctccatt acttatcgcg
     9841 ccacagttga taaatcttgc ccggtgaata tgactaatca cgtctatttc aatcttgacg
     9901 gcgagcagtc tgacgtgcgc aatcacaagt tgcagattct ggcgggcgaa tatctgccgg
     9961 ttgatgaagg cggcattccg cacgacggcc tgaaatctgt cgccggaacc tcttttgatt
    10021 tccgcagcgc caaaatcatc gccagtgagt ttcttgccga cgacgatcag cgcaaagtga
    10081 aaggttacga tcacgcattc ttgttacagg ccaaaggcga tggcaagaaa gtggcggcgc
    10141 atgtctggtc agcagatgaa aaattgcagc tgaaggtcta caccaccgct cccgctcttc
    10201 aattctactc cggcaacttc ctcggcggca caccgtcgcg gggaaccgaa ccttacgccg
    10261 actggcaagg actggcgttg gaaagcgagt ttctgccgga cagcccgaac caccctgaat
    10321 ggccgcaacc ggactgcttc ctgcgtcctg gcgaagagta ttccagcctg acggaatatc
    10381 agtttattgc tgagtaatgt taagccctcg tcatgagggc tttatcttca tattgttcaa
    10441 atcaccagca aacaccgaca tatttgcaac tcaatattca caacaacctt acactgcgcc
    10501 actattttcg ctatggttat gcgtaagcat tgctgttgct tcgtcacggc aatataatga
    10561 gaattattat cataaaaaga taatttgagg agtaagtata tggctgtaac taagctggtt
    10621 ctggttcgtc atggcgaaag tcagtggaac aaagaaaacc gtttcaccgg ttggtacgac
    10681 gtggatctgt ctgagaaagg cgtaagcgaa gcaaaagcag caggtaagct gctgaaagag
    10741 gaaggttaca gctttgactt tgcttacact tctgtgctga aacgcgctat ccataccctg
    10801 tggaatgtgc tggacgaact ggatcaggca tggctgcccg ttgagaaatc ctggaaactg
    10861 aacgaacgtc actacggtgc gttgcagggt ctgaacaaag cggaaaccgc cgaaaagtat
    10921 ggcgacgagc aggtgaaaca atggcgtcgt ggttttgccg tgactccgcc ggaactgact
    10981 aaagatgatg agcgttatcc gggtcacgat ccgcgttacg cgaaactgag cgagaaagag
    11041 ctgccgctga ctgaaagcct ggcgctgacc attgaccgcg tgatccctta ctggaatgaa
    11101 actattctac cgcgtatgaa gagcggtgag cgcgtgatca tcgctgcaca cggtaactct
    11161 ttacgtgcgc tggtgaaata tcttgataac atgagtgaag aagagattct tgagctgaat
    11221 atcccgaccg gcgtgccgct ggtgtatgag ttcgacgaga atttcaaacc gctgaaacgc
    11281 tattatctgg gtaatgctga cgagatcgca gcgaaagcag cggcggttgc taaccagggt
    11341 aaagcgaagt aaacgtcatt cgtttaaaaa tgagaaagcc gactgcaagt gagtcggctt
    11401 ttttgttgct aacaatggag cacattgcct gatgcgacgc tgcgcgtctt atcaggcctg
    11461 tggtgattca tcggatacgc cactctgacg gcgcatccga caattaaacc ttacccgcga
    11521 cgcgctttta ctgcattcgc cagttgacgt aacagagcat cggtatcttc ccagccaatg
    11581 caggcatcgg tgatgctctt accgtaggcc agcggctccc cgctatcgag gctctgattg
    11641 ccttccacca gatggctttc caccatcacg ccaataatgg ccttttcgcc accggcaatc
    11701 tgctggcaaa cgtcagcaca aacatccatc tgctttttga attgtttgga cgagttagca
    11761 tggctgaaat cgatcatcac ctgtgctggc aggcctgctt tgttcagccc ttctttcact
    11821 tcagcaacgt gcttcgcgct gtagttaggc tctttaccgc cgcgcagaat gatatggcaa
    11881 tcaccgttac cgctggtatt cacaatcgcc gaatgccccc atttagttac ggacaggaag
    11941 cagtgcggcg caccggcggc attaatggca tcgatagcca ctttaatcgt accgtctgtg
    12001 ccatttttaa aacctaccgg acaagaaaga ccagacgcca gttcgcggtg cacctgcgat
    12061 tccgtggtac gtgcaccaat tgcgccccag ctcatcaggt cagcgagata ctgaggagtg
    12121 atcatatcga gaaactcacc cgccgctggc agaccgctgt cgttaatatc aagcagcaat
    12181 ttacgggcta tacgcagacc gtcgttgatc tggaagctgt tatccatatg cggatcgtta
    12241 atcaaccctt tccagcccac cgtggtacgc ggcttttcaa aatagacgcg cattacgatt
    12301 tccagctcat ctttcagctc ttcacgcagc gccagcaagc gagtggcata ctctttagcc
    12361 gcgacaggat catgaattga gcacgggcca atcaccacca acaggcgatc atcattacct
    12421 ttcaggatct tatggatcgc ttttcgggca tgggcaaccg tattcgcggc attttcagtt
    12481 acggggaatt tttccagcaa tgcgacagga ggaagtaact ctttgatttc tttgatgcgt
    12541 aaatcgtcgt tctgataatt catgtctgtt ccagtgttgc catacttatc ttagtgaatg
    12601 caatacttcc aatctatatc ttccgtcaga atgtgtaaac ggggttttac actatgaacg
    12661 gattaatcct ggaattagca agaaaaacgc cagattgtcg cgaaaaacga gatctctcct
    12721 acaatttcta actgtaactc ctttgtttgt taattatttc aagattctct gctgcgtttc
    12781 ataaactggc tgaaatctta aaccaatgcc ttatattcac ctgcaaatgc actgttggaa
    12841 gaggttatcc gacataacga ccataacagg agcatcctat gaaaatgaca aaactggcca
    12901 cattatttct gactgccact ctaagccttg ccagcggtgc cgcactggct gccgatagcg
    12961 gagcgcaaac taataacggc caggcaaacg ccgcagctga tgcgggccag gtagcccctg
    13021 acgcccgtga aaatgtcgcg ccaaataacg tcgacaataa cggggtaaat accggttctg
    13081 gcggcacaat gctgcattcg gatggttctt caatgaacaa tgacggaatg accaaagatg
    13141 aagagcacaa gaacaccatg tgcaaagatg gtcgatgtcc ggatatcaat aaaaaggtac
    13201 aaatcggtga tggcatcaat aatgatgtcg ataccaaaac cgacggtacc acgcagtaac
    13261 cctgcaaaat agccaataat gggagagcgg agtctctccc ttttaatttt tcacttctaa
    13321 aagcagtatg atgtcataaa gttaagtgag aataattaag ggatgactcg tcatggcgca
    13381 ctcacactca cacacgtcct cacacctgcc agaagataat aatgctcgtc gcttgttgta
    13441 tgctttcggc gtgactgccg ggtttatgct ggtagaagtc gttggtggtt ttctttctgg
    13501 ttctctggca ttgctggccg atgcgggtca tatgttgacc gatactgccg ccctgctttt
    13561 cgccctactc gccgtgcaat tttcacgtcg tcctcccacc attcgccaca ctttcggttg
    13621 gctaagactg accacgctcg cggcgtttgt gaatgccatc gccctcgtgg taattaccat
    13681 tttgattgtc tgggaggcga tagaacgctt ccgcacgccg cgtccggtcg agggcggcat
    13741 gatgatggca attgccgtgg ctgggttgct ggcaaatata ctttctttct ggttacttca
    13801 tcacggcagc gaagagaaaa acctcaacgt acgagcagcg gcattgcatg tgctcgggga
    13861 cctgttgggt tcggttgggg ctattattgc cgcgttgatt attatctgga ccggctggac
    13921 acctgctgac cccattctct cgatagtggt gtcgcttctg gttctgcgta gcgcgtggcg
    13981 actgttgaaa gatagcgtga atgaattact tgaaggtgca ccggtatcgc tggatatcgc
    14041 tgaactgaag cgtcgtatgt gccgggaaat cccggaagtc cgcaatgtgc accatgtaca
    14101 tgtatggatg gtaggcgaga agccggtgat aacgctgcat gtgcaggtga tcccgccaca
    14161 cgatcacgat gccttgttgg atcagatcca acactacctg atggatcact atcagattga
    14221 gcacgccacc attcagatgg aatatcaacc ttgtcatggg ccagactgcc acctgaatga
    14281 aggggtgtct ggccattcac atcatcacca ttaatgggac agcgcgcgtg agcctctttc
    14341 acgtgcgctg ttgatccaca tccgactgcc gttgagcgca ataaaggtca ggatgatgta
    14401 ctccagagac atggcgtaaa cgccctgaag tgcaaaaata acgacgctaa tcacgttaat
    14461 aatcacccac aacagccagt tttccacata cttacgcgtc atcagaatca ttgccacgat
    14521 agataacacc atcatgcatg aatcccagaa cgggaaagcg tccggttgca gttcaggcat
    14581 cacaacctgt aatcctaatg cttgcatgat catgactgcc acgcgggtca aaaatgcaaa
    14641 caccggattg ataaataccg tcatcagacc aatcgaaaca acgcaaaccg ccaaccagct
    14701 gagcgccttc ggcaatggta accagcgaat tttcaactcc gcctcgttct gactggtttg
    14761 tcgcgaccac gcataccaac cgtaaatatt cgcggcaaag aaaaacacct gtaatagcag
    14821 gctggcatac agctgaatct gaaagaaaat aatgccaaac aaggtgacgt tgatcaggcc
    14881 aaagaagtag ttgctgatct tctccagact ggcaaggcca atacacagca acccggcgat
    14941 cgtgcctacc gcttcgatcc atgagagatc ataaccgcct gcccctattg gtatatgtac
    15001 caggatattc tgcacactaa aaaaatccat ctttccccca aagcgtaacg ttacgcctta
    15061 ttattcgtta tccacgtagt gtagccgcaa aatccagcat acgattgagc ggcaccagcg
    15121 ccctctctcg cagcctttca tcaagaacct cgtgattgct tccttcctgt tctaatgcct
    15181 ctgcgatgga ctgaaggcca ttcatggcca tccacggaca atgcgcgcag
//
LOCUS       DAJVRF010000098        15084 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJVRF010000098 DAJVRF010000000
VERSION     DAJVRF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.098"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..781
                     /locus_tag="OQN13_002777"
     CDS             <1..781
                     /locus_tag="OQN13_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616088.1"
                     /translation="NKESLFFMSVMELRDLLIALESRLHQKHKLNAAARLQYEQARNK
                     VIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLHDGSKCELVVNEL
                     QIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYDTYSQPEWKHN
                     LFDHYLAVLYRFKDESGKEQFSGTVVKTREATPGKEIEAITRRMLDFSPRLKKLAGVP
                     CQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
     gene            complement(984..1337)
                     /locus_tag="OQN13_002778"
     CDS             complement(984..1337)
                     /locus_tag="OQN13_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCS1616089.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1475..3121)
                     /gene="groL"
                     /locus_tag="OQN13_002779"
     CDS             complement(1475..3121)
                     /gene="groL"
                     /locus_tag="OQN13_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCS1616090.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3165..3458)
                     /locus_tag="OQN13_002780"
     CDS             complement(3165..3458)
                     /locus_tag="OQN13_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCS1616091.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3734..4990
                     /gene="yjeH"
                     /locus_tag="OQN13_002781"
     CDS             3734..4990
                     /gene="yjeH"
                     /locus_tag="OQN13_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCS1616092.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5012..5482)
                     /gene="fxsA"
                     /locus_tag="OQN13_002782"
     CDS             complement(5012..5482)
                     /gene="fxsA"
                     /locus_tag="OQN13_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCS1616093.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            5819..7255
                     /gene="aspA"
                     /locus_tag="OQN13_002783"
     CDS             5819..7255
                     /gene="aspA"
                     /locus_tag="OQN13_002783"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCS1616094.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7373..8674
                     /gene="dcuA"
                     /locus_tag="OQN13_002784"
     CDS             7373..8674
                     /gene="dcuA"
                     /locus_tag="OQN13_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCS1616095.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8790..9128
                     /gene="cutA"
                     /locus_tag="OQN13_002785"
     CDS             8790..9128
                     /gene="cutA"
                     /locus_tag="OQN13_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCS1616096.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9104..10801
                     /gene="dsbD"
                     /locus_tag="OQN13_002786"
     CDS             9104..10801
                     /gene="dsbD"
                     /locus_tag="OQN13_002786"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCS1616097.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10838..11413
                     /locus_tag="OQN13_002787"
     CDS             10838..11413
                     /locus_tag="OQN13_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1616098.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11520..11595
                     /locus_tag="OQN13_002788"
     tRNA            11520..11595
                     /locus_tag="OQN13_002788"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11553..11555,aa:Phe,seq:gaa)
     gene            12212..13749
                     /gene="cadC"
                     /locus_tag="OQN13_002789"
                     /pseudo
     CDS             12212..13749
                     /gene="cadC"
                     /locus_tag="OQN13_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
     gene            14114..>15084
                     /gene="cadB"
                     /locus_tag="OQN13_002790"
     CDS             14114..>15084
                     /gene="cadB"
                     /locus_tag="OQN13_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCS1616099.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         3708 a         3764 c         3616 g         3996 t
ORIGIN      
        1 caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc tgattgcgct
       61 ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc agtatgagca
      121 agctcgcaat aaagtcatta agaaaatggc agaaaacatc ccagaaattc tggttgatga
      181 gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg ataatcaagg
      241 cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt tagtcgtcaa
      301 tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata atgcggaaat
      361 gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat acgacgttga
      421 ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat ggaaacataa
      481 cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca
      541 atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa tagaggcgat
      601 cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg tgccatgtca
      661 ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg atcaatgctt
      721 acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac aggcgaaata
      781 atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg ataacgtgat
      841 cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc tcaagcattc
      901 aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca ataatacgtt
      961 tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc gataatctgg
     1021 ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat gccataaatc
     1081 acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc gcgcatagaa
     1141 ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt cgcagtacca
     1201 atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg accggcagca
     1261 ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac aatccctgct
     1321 aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac tcgcgtcgtc
     1381 cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac atttctgccc
     1441 gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc atgccaccca
     1501 tgccgcccat accgccagca gcgcctaagt cagctgcatc atttttcggc aggtcggtaa
     1561 ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc agagcagaac
     1621 gggttacttt ggttgggtcc aggataccca tgtcgatcat gttgccgtat tcttcggttg
     1681 ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca acagacggtt
     1741 cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc agtgcaactt
     1801 tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta gacgctacgc
     1861 ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca cgggtcgcgt
     1921 gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta gcagcaccta
     1981 ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt ttttcacggt
     2041 cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg ccctggattg
     2101 cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc acaacacgtt
     2161 tagcctgacc caggtcttcc agggttgctt tttccagttc cataccgatc tcttcagaga
     2221 tcacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga cgatcgccga
     2281 agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta acaaccagag
     2341 ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg cctgctttgg
     2401 caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg tcagccagca
     2461 ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg aagtaaggag
     2521 acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc tgcagaccgg
     2581 taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct tcagcgatca
     2641 gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca atcgctttag
     2701 agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta acggctttgt
     2761 cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc agaccctcag
     2821 tgatgatagc ctgagccagt acagttgcag tggtggtacc gtcgcctgca gcgtcgttcg
     2881 ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg tcttccagtt
     2941 cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg aaagatttat
     3001 ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct gccagtacgt
     3061 ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct ttagctgcca
     3121 ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc ttcaacaatt
     3181 gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt ctcagatttc
     3241 acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag cggcttcact
     3301 tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg ggtggattta
     3361 gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac ttctttacgc
     3421 ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc tttgagaaag
     3481 tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt gaccagagaa
     3541 atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg atcagcacaa
     3601 aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat cgccgtcata
     3661 agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt tgggcgcaat
     3721 cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg gcattggcct
     3781 gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag ctgcacaggt
     3841 agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt tcccgattgc
     3901 gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg cgcacttcgt
     3961 cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt tatcggtcat
     4021 tcccgtgggt ttgcctgccg cgctacaaat tgccgccggg ttcggccagg caatgtttgg
     4081 ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg tgtggtatat
     4141 cggtactcgc ggtgccagtt ccagtgctaa tctacaaacc gttattgccg gacttatcgt
     4201 cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata tccccttccc
     4261 ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga tgttctggtg
     4321 ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc cagagcgtga
     4381 ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact ggggctgtac
     4441 ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag catcgcttcc
     4501 aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg taattggcta
     4561 tctgacctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc tggtctggtc
     4621 gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc atatcccgaa
     4681 taatgccctc aatgcggtgc tcggctgctg cgtggtgagc actttggtga ttcatgcttt
     4741 agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta tgatttatct
     4801 gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac tggcggtggt
     4861 tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc tctatgcgct
     4921 gattatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg aaaatggcat
     4981 aaccacataa tccggcgttt cgacattaat cctagcgatc gtctttatga tcaaggcggt
     5041 cgcggtcatc atcctttcgc tggtactcac catcaaaagt attaccgcca ccggtcccgg
     5101 cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac ttcactgtca
     5161 gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg aaaaagcccg
     5221 gtaataaaag cagcaaaccg gcaataatca gcgaaacact tttaatcatc tccgccgcag
     5281 ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag ccctggttac
     5341 gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc agcaataccc
     5401 ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa aggaaaatgg
     5461 caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta aacccggcta
     5521 cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa atttcaagat
     5581 gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac aaattaataa
     5641 ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc ctcaagcagc
     5701 atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa acattcgaaa
     5761 ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga aggttcacat
     5821 gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc cagctgatgc
     5881 ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca acaacaaaat
     5941 cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg cagctatggc
     6001 aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg ccgcatgtga
     6061 tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct accagggcgg
     6121 cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg gtctggaact
     6181 gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg ttaacaaatg
     6241 tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact cttccctgat
     6301 taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag ctgtcgaatt
     6361 ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga tgaccctcgg
     6421 tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca tccaacgtac
     6481 cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg gtctgaacac
     6541 gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg gcttcccatg
     6601 cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg ttatggttca
     6661 cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc tgcgcttgct
     6721 ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc aggcgggctc
     6781 ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc aggtatgctt
     6841 caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc agctgcagtt
     6901 gaacgttatg gagccggtca ttggccaggc tatgttcgaa tccgttcaca ttctgaccaa
     6961 cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca aagaagtgtg
     7021 cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt tcatcggtca
     7081 ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg tacgtgaagt
     7141 cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct ccgtacagaa
     7201 tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac agtaatcgta
     7261 cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt gtgagcagta
     7321 acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta atatgctagt
     7381 tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg ggggaatagg
     7441 tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg ttaaacccgg
     7501 taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta tttctgccat
     7561 gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc tgcgccgtaa
     7621 cccgaaatac atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa tctttgctgg
     7681 tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga aggaacaagg
     7741 cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg cgatcaccgc
     7801 atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc atggcatcag
     7861 ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc tggtgatgtc
     7921 cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt atcgcaagcg
     7981 tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca aatccggtgc
     8041 aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct atgcaatcat
     8101 caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca acgcaatcct
     8161 gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg ataccgacaa
     8221 catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta tcctgggtgt
     8281 tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag ataccgctgg
     8341 tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg cttctgctct
     8401 gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg cactgaacgt
     8461 ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca ttctgccgac
     8521 ctatccgacg ctggttgctg cggtacagat ggatgacacg ggtacaaccc gtatcggtaa
     8581 attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc tggccgtttg
     8641 cttcggcttc gtgctgggta gcttcatgct ataatgacca atcgcggggc gttcacgccc
     8701 cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga cctctctctt
     8761 gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg aataccgcgt
     8821 ctgtcgtggt gctatgtacc gcaccggatg aagcgacagc ccaggattta gccgccaaag
     8881 tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc tctctctatt
     8941 actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa actaccgtat
     9001 ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa accccggaac
     9061 ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc aacgcatctt
     9121 tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg acgcgccggg
     9181 acgttcacat tttgtccccg cggatcaagc ctttgctttt gattttcagc aaaaccaaca
     9241 tgacctgaat ctgacctggc agatcaaaga cggttactac ctctaccgta aacagatccg
     9301 cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg tctggcatga
     9361 agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc ccgtaaccat
     9421 caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg ctgatgccgg
     9481 tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg ccaacaacgc
     9541 agcgtcacag cctgtgtctg ttccgcagca agagcagcac accgcgcaat tgcccttttc
     9601 cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg tgctgccaat
     9661 gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca ctgccagagc
     9721 attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg cgctgggtct
     9781 ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat acgtgctcat
     9841 tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt ttactctaca
     9901 actcccctct tcgctgcaaa cacatctcac attgatgagc aatcgccaac agggcggctc
     9961 acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac catgcaccac
    10021 cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt ggctgggcgg
    10081 cggcatgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa ttaccgtctt
    10141 tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa ccgcgtttgg
    10201 ttttgtgatc ctcgcactgc cgatcttcct gctggagcga gtgattggtg atatatgggg
    10261 attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta tcaccagcct
    10321 acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag cggcattggt
    10381 tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc aaactcagac
    10441 gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc tcgttgaagc
    10501 caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct gtaaaaagtt
    10561 tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca cggtcttact
    10621 tcaggccaac gtcactgcta acgacgcaca agatgtggcg ctgttaaagc atcttaatgt
    10681 ccttggacta ccgacaattc tcttttttga cggacaaggc caggagcatc cacaagcacg
    10741 cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc gccaaccgtg
    10801 aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag atgtactggg
    10861 agaagccctg aaattattag aattacaagg gattgccaac accacgctgg agatggttgc
    10921 tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca aagaggcaat
    10981 cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc gtcagctgat
    11041 gcttgacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg cgttatcgga
    11101 gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta cgttttatcc
    11161 cgaccctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg cctacgattt
    11221 cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag caaagcagat
    11281 ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc aggcggatgt
    11341 cgacaccgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc gccagggtgg
    11401 tgcactgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta agcagttgaa
    11461 tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg cgccccgttg
    11521 cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt ggttcgattc
    11581 cgagtccggg caccaaattc atataaacgg acctccacgg aggtccgttt ttcgtttcag
    11641 gacgccacga tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa ccagattctc
    11701 cccacatcac cagcattttg cgggtatatc ccaattcggg aaaatttgtt cctgagcgat
    11761 agcactgact gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt caaatgacgt
    11821 catcaggagc gtaacgcatc cataaagcac aacaccggtc tgaacggcga ctgatgagat
    11881 tttctgaatg agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa gactaacaat
    11941 ccattaaaat agtaagcgct ccggacaatt ttccatggat tattttctac acatttttct
    12001 ttggcaaaga tgatgaattt tgatggtaag gaatattact tctggttctc agtaaaatcc
    12061 tttcgtaata ctatgtaatc aagaagttta tggctggtaa aaataacgtc ttgcattcac
    12121 caataacatg taaataaacc catctattga tggtaaaaat aggtcatggc aattatcatt
    12181 gcaccattcc cttttcgaat gagtttctat tatgcaacaa cctgtagttc gcgttggcga
    12241 atggcttgtt actccgtcca taaaccaaat tagccgcaat gggcgtcaac ttacccttga
    12301 gccgagatta attgatcttc tggttttctt tgctcaacac agtggcgaag tacttagcag
    12361 ggatgaactt atcgataatg tctggaagag aagtattgtc accaatcacg ttgtgacgca
    12421 gagtatctca gaactacgta agtcattaaa agataatgat gaagatagtc ctgtctatat
    12481 cgctactgta ccaaagcgcg gctataaatg aatggtgccg gttatctggt acagcgaaga
    12541 agagggagag gaaataatgc tatcttcgcc tccccctata ccagaggcgg ttcctgacac
    12601 aaattctccc tcccacagtc ttaccattca aaacaccgca acgccacctg aacaatcccc
    12661 agttaaaagc aaacgattca ctaccttttg ggtatggttt ttttcctgtt gtcgttaggt
    12721 atctgtgtag cactggtagc gttttcaact cttgatacac gtcttcctat gagcaaatcg
    12781 cgtattttgc tcaatccacg cgatattgac attaatatgg taaataaaag ttgtaacagc
    12841 tggagttccc cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt ggcgacatca
    12901 cttaacacct tctccacctt tatggtgcat gacaaaatca actacaacat tgatgaaccg
    12961 agcagttccg gtaaaacatt atctattgcc tttgttaatc agcgccaata ccgtgctcaa
    13021 caatgcttta tgtcggtaaa attagtagac aatgcagatg gttcaaccat gctggataaa
    13081 cgttatgtca tcactaacgg taatcagctg gcgattcaaa atgatttact ggagagttta
    13141 tcaaaagcgt taaaccaacc gtggccacaa cgaatgcagg agacgctcca gcaaattttg
    13201 cctcatcgtg gtgcgttatt aactaatttt tatcaggctc atgattattt actgcatggc
    13261 gatgacaaat cattgaatcg tgccagtgaa ttattaggtg agattgttca atcatcccca
    13321 gaatttacct acgcgagagc agaaaaagca ttagttgata tcgtgcgcca ttctcaacac
    13381 cctttagatg aaaaacaatt agcagcactg aacacagaaa tagacaacat tgttacactg
    13441 ccggaattga acaacctgtc cattatatat caaataaaag cggtcagtgc tctggtaaaa
    13501 ggtaaaatag atgagtctta ccaggcgata aatactggca ttgatcttga aatgtcctgg
    13561 ctaaattatg tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg ggaagcagct
    13621 gatgcatatc tcaccgcctt taatttacgc ccaggggcaa acacccttta ctggattgaa
    13681 aatggtattt tccagacttc tgttccttat gttgtacctt atctcgacaa atttctcgct
    13741 tcagaataag taactccggg ttgatttatg ctctggaata tttgttgttg agtttttgta
    13801 tgttcccatt ggtataatat gttgcggcaa tttatttgcc gcataatttt tattacataa
    13861 atttaaccag agaatgtcat gcaatccatt gtaaacattg aatgtttatc ttttcatgat
    13921 atcaacttgc gatcctgatg tgttaataaa aaacctcaag ttctcactta cagaaacttt
    13981 tgtgttattt cacctaatct ttaggattaa tccttttttc gtgagtaatc ttatcgccag
    14041 tttggtctgg tcaggaaata gttatacatc atgacccgga ctccaaattc aaaaatgaaa
    14101 ttaggagaag agcatgagtt ctgccaagaa gatcgggcta tttgcctgta ccggtgttgt
    14161 tgccggtaat atgatgggga gcggtattgc attattacct gcgaacctag caagtatcgg
    14221 tggtattgct atctggggtt ggattatctc tattattggt gcaatgtcgc tggcgtatgt
    14281 atatgcccga ctggcaacaa aaaacccgca acaaggtggc ccaattgctt atgccggaga
    14341 aatttctcct gcatttggtt ttcagacagg tgttctttat taccatgcta actggattgg
    14401 taacctagcg attggtatta ccgctgtatc ttatctttcc accttcttcc cagtattaaa
    14461 tgatcctgtt ccggcgggta tcgcctgtat tgctatcgtc tgggtattta cctttgtaaa
    14521 tatgctcggc ggtacctggg taagccgttt aaccactatt ggtctggtgc tggttcttat
    14581 tcctgtggtg atgactgcta ttgttggctg gcattggttt gatgcggcaa cttatgcagc
    14641 taactggaat actgcggata ccactgatgg tcatgcgatc attaaaagta ttctgctcta
    14701 cctgtgggcc ttcgtgggtg ttgagtccgc agcagtaagt actggtatgg ttaaaaaccc
    14761 gaaacgtacc gttccgctgg caaccatgct gggtactggt ttagcaggta ttgtttacat
    14821 cgctgcgact caggtgcttt ccggtatgta tccgtcttct gtaatggcgg cttccggtgc
    14881 tccgtttgca atcagtgctt caactatcct cggtaactgg gctgcgccgc tggtttctgc
    14941 attcaccgcc tttgcgtgcc tgacttctct gggctcctgg atgatgttgg taggccaggc
    15001 aggtgtacgt gccgctaacg acggtaactt cccgaaagtt tatggtgaag tcgacagcaa
    15061 cggtattccg aaaaaaggtc tgct
//
LOCUS       DAJVRF010000099        14965 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJVRF010000099 DAJVRF010000000
VERSION     DAJVRF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.099"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="OQN13_002791"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="OQN13_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCS1616100.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1931)
                     /locus_tag="OQN13_002792"
     CDS             complement(1608..1931)
                     /locus_tag="OQN13_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCS1616101.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="OQN13_002793"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="OQN13_002793"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCS1616102.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..6106)
                     /gene="pat"
                     /locus_tag="OQN13_002794"
     CDS             complement(3446..6106)
                     /gene="pat"
                     /locus_tag="OQN13_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCS1616103.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="OQN13_002795"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="OQN13_002795"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCS1616104.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="OQN13_002796"
     CDS             complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="OQN13_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCS1616105.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7531..8568
                     /locus_tag="OQN13_002797"
     CDS             7531..8568
                     /locus_tag="OQN13_002797"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS1616106.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8616..9305)
                     /gene="ung"
                     /locus_tag="OQN13_002798"
     CDS             complement(8616..9305)
                     /gene="ung"
                     /locus_tag="OQN13_002798"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCS1616107.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9610..9993
                     /gene="grcA"
                     /locus_tag="OQN13_002799"
     CDS             9610..9993
                     /gene="grcA"
                     /locus_tag="OQN13_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCS1616108.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="OQN13_002800"
     CDS             complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="OQN13_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCS1616109.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10739..11620
                     /locus_tag="OQN13_002801"
     CDS             10739..11620
                     /locus_tag="OQN13_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1616110.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="OQN13_002802"
     CDS             complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="OQN13_002802"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCS1616111.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13295..14032
                     /gene="trmN"
                     /locus_tag="OQN13_002803"
     CDS             13295..14032
                     /gene="trmN"
                     /locus_tag="OQN13_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCS1616112.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14017..>14965)
                     /locus_tag="OQN13_002804"
     CDS             complement(14017..>14965)
                     /locus_tag="OQN13_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCS1616113.1"
                     /translation="PLLPVHLSRVANLEFNQFPPTALYHPQARNFLLTEALRGEGAYL
                     KRPDGTRFMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPM
                     IYEKLLGLGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGAN
                     RMASNSLLECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHEL
                     RLFMWDYVGIVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIV
                     RCAMMRKESRGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3612 a         3785 c         3921 g         3647 t
ORIGIN      
        1 aaaggccatc ctgacggatg gcctttttgc attggcgcag aaaaaaatgc ctgatgcgac
       61 actgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggt ttccccaact
      121 tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcacaatg atatgcaaca aatgtataac
      241 attcccttta ccaaaaaaaa taaacaaaag cgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc aattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tacttcgccc acatcttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgctatcgg tgtgtatgat gtgtttcggc tcactggtaa tcgcctgttt gcctggttat
      661 gaaacaatcg gtacgtgggc tccggcatta ttgcttctcg cgcgtttatt tcagggatta
      721 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 cagttagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac tacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgtcaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggcctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgcggcg caagcttcag ttgtggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttca ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gataagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccgaattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacagcaaat tcggcatcga tgttatcagg atcggaaatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgtgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct
     3901 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     3961 cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg
     4021 gcgagatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     4081 gaatatccag acgctgaatt tccgggcaat cgacaatcaa gttggaaacc tgcaccagaa
     4141 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac
     4201 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     4261 cgacggcttg atcttcagga cgccactcca caccgccttc acccagcatg atcaacggcc
     4321 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagca cggttagcca
     4381 tactttgcac caacaggccg tggacccgcg cctgtggcca ggccattttt acgcgatcga
     4441 aaatagcgtt cgccgcttgc tggacttcat tggctgtacg caggtaaagc atgacgccct
     4501 gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa
     4561 tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggta gggagcgtgt
     4621 tcatgccata cgcttgcagg atgggctgaa cttcatgggt atcgagcgac gtagcccctt
     4681 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     4741 gcaacgtcgg cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa
     4801 aagcagtgat ggttccttcc ggggtacggt aggtcggcag cccggcttcg ctgaataaac
     4861 gtcgtgcctc ttgcgaggag tgctcgccgc accagttcgt cagcaaagag acatatttgc
     4921 tgcggggatg atgctttacc gcttcaatta atacttgcgc gctttctgtt gcgggagcag
     4981 cggcgctggg cgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     5041 ccagcgtttt gacataatgc tcgctgctgg cgtcatcgcg cagatcgaga gggttagaga
     5101 ctgccacatg ttctggcaat gcatcgcgca atttctggca ggtttcttcg cttagcgttg
     5161 ccagcttgcc attgcgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     5221 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     5281 ccgaaaacag ttcgtgggta tcctgtaccc gcaacaaacc ggcacgctga atagccgcat
     5341 cccatgccgg gtccattcct gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc
     5401 cgcttttaat caccagaatc ggtttattac gcgaggcact acgggctgcc gaaacaaagc
     5461 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     5521 ccagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     5581 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggctgccg
     5641 attgtgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca
     5701 gaccttgcca gggagccagt aaacccagac tgtttggtcc aagcaggcgc atgttatggc
     5761 gcagggcgca ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg
     5821 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac
     5881 ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag gccaacacac
     5941 ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cttcccgcca
     6001 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgacgcg ccaattaccg
     6061 ctatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca
     6121 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt
     6181 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgct
     6241 gccgccccgg tatcgccggc catatctaac agtgcgatgg ctacctcggc agtacaatat
     6301 tggcctgcag cctgggcttc acgcaggcga taggcagaaa gccgggaaag atcgacggaa
     6361 atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc ttccggccag
     6421 gtaccatcga gcatgataaa cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc
     6481 cgttgctcat cagcatacga ggcgggaaag accaccattg gctgataatc cgggttttgt
     6541 accagatcca gcaaatcctg cgagggttcg gtacgcgacc attgaaacgc aacggtatca
     6601 ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac
     6661 atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat
     6721 ttctctggca aaagacagcg ttggcagcga tgaacgcgat taccgcgggc aagaaaagga
     6781 cgtgttgccc gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga
     6841 gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcaccccggt
     6901 aagattaaag agattcgttc agccagctat cgaacggcgc tttcggtact gcgccattaa
     6961 gcatgtcgac aacctgaccg tttttgaaaa tcatgatcgt cggtatacta cgaattccaa
     7021 agcgactgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc
     7081 tacgctcttg cgcgacatct tcaaaaattg gtgcgaaatt acggcagggg ccgcaccacg
     7141 gtgcccagaa gtcgatcacc acaggtagat catccttcag caatttgtcg agtgtttcac
     7201 cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg
     7261 catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat
     7321 tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta
     7381 atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat tcaataggta
     7441 tgatagtcgc aggctttggt acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc
     7501 gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc
     7561 aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg
     7621 cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc
     7681 gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagattc gccgtggcgc
     7741 acggtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt
     7801 ggtaaaagtt ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc
     7861 tacggcgaaa atgcctgtca ggcactgttc cagagccgtc cggaagcaat tgttcgcgcc
     7921 tggtttatcc agagcgtaac gccacgtttt aaagaagcct tgcgctggat ggcggcaaac
     7981 cgcaaagcgt accatgtggt ggatgaagct gaattgacaa aagcgtcagg cacggaacat
     8041 cacggcggcg tttgcttcct gatcaaaaag cgtaacggta caaccgtgca gcagtgggta
     8101 agccaggcag gcgcgcagga ttgcgttctg gcactggaaa atgaatctaa cccgcataac
     8161 ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggtgtcgt ggtgcaggat
     8221 gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt
     8281 cagccgatta ctggcgacaa cattgttaac gtgctggatg atttccgtca ggcaggttac
     8341 accgtagtga ccacttccag cgagcagggt aaaccgctgt tcaaaactag tctgccagcg
     8401 aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg
     8461 aacgatctgc gcgtgaagat tgacggtacg ggcaacgttg ccgggctgaa tatctctgtc
     8521 gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg
     8581 gtggcaactc tgccatccgg catttccccg caaatttact cactctctgc cggtaatact
     8641 ggcatccagt caatcggcgt ctcgccacgt tgttccagcc actgatttgc cagcacaaaa
     8701 tggttgcagc caaagaatcc acgatgcgcc gaaagcggcg acggatgcgg tgctttcagt
     8761 acatgatggc gttgcttatc tataatcgcc cctttctttt gcgcatgtga tccccacaac
     8821 aaaaacacca cgccttcgcg gtgctggtta atcaggctga tgactttatc agtaaacgtc
     8881 tcccaaccga ggctggcgtg ggaatgcgcc tgacctgcgc gaaccgtcaa cacagtattg
     8941 agcagcagaa cgccctgacg cgcccagctt tcgagatagc catgattagg gcgggtgaag
     9001 cccggaatag tattttccag ctctttatac atattcaata atgacggagg aatggcaatg
     9061 ccgggacgaa cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata aggatcctgg
     9121 ccgagaatca ccactttaac gtcacccaac tcggtaaagc ggaacgcgtt aaagacatct
     9181 ttttgtggtg gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgaagggta
     9241 ttaagaaaat aaggttgttg cttctcttca gccagcacgt catgccaggt taattcgtta
     9301 gccatctcgc tctcctgcga atcttcaatc cgcctagctt aactgcttct ttctacagaa
     9361 caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt
     9421 tcacacccgc ataagttgat gtaaaacaat aaaatccatt tacgaccaca atcgatgtgt
     9481 ggtttttatt gatttaaatc aaagaatcaa gggtggttga ggagtatata tacactcaag
     9541 caacaatggt tttaccaatt ggccgcgaca ggctgaacaa atcaaataat tttgccgggg
     9601 aggcatcaca tgattacagg tatccagatt actaaagccg ctaacgacga tctgctgaac
     9661 tctttctggc tgctggacag cgaaaaaggc gaagcacgtt gcatcgttgc aaaagcaggt
     9721 tatgcagaag atgaagtggt tgcagtaagc aaactgggtg acattgaata ccgtgaagtt
     9781 ccagtagaag tgaaaccaga agttcgcgtt gaaggtggtc aacacctgaa cgttaacgtt
     9841 ctgcgtcgtg aaactctgga agatgcagtt aagcatccgg aaaaatatcc gcagctgacc
     9901 atccgtgtat ccggttatgc agttcgcttt aactctctga ctccggaaca gcagcgcgac
     9961 gttatcgctc gtacctttac tgaaagcctg taaggcttaa gtagcaagtt actaagcgga
    10021 agagggcgac ctcttccgct ttttttcgtt aatagaaaat gcgtaccgcg caatagacca
    10081 gtaacagggc aagcacgata tttaactggc gaccatactg gcgaaacaat cgctgaaaca
    10141 gatgccccgc cagcgcccag cacacattgc caaacgtccc aatcatcgcc agcaaaacgc
    10201 tgacgccaac tacccagctt agcgcctgtg tttgcggcag aacaaacgtc gacagtgccg
    10261 taacaccgta caaaatgatt ttgacgttca caaactgcaa agcaaagctg gcccaaaagc
    10321 tgattggttt tgcctgaagt ccgtcttcct ttgttgggct ggtggcgatt ttccacgcca
    10381 gccagacaat atatgccgcc cccgcccaac tcaaaaggtg taccgctgcc gggtcaatca
    10441 ctgccagtga aaatgaaatg cccgcacaca gtaacatcac aatcaaaaat cccagactca
    10501 tccctgccag cacgcgggta ctttgacgaa atccatgcga cgtagcagag ctaagggcga
    10561 gaatattgtt cggtcctggc gtcatagcgg taatcagggt gtaagtccaa aaagcactta
    10621 aaagggtcgg tgtcacttgt tatctcctct gttttggtag acagtacccg acacggaaca
    10681 tttaaaaaaa tgaatgtttt tatattctag attcgatatt ttcgataggt ttggggttat
    10741 ggatctgcgc cgttttatta cgcttaaaac cgtggtggaa gagggttcct ttttgcgagc
    10801 ttcgcaaaaa ttgtgctgta cacaatcgac ggtgactttt catattcagc agcttgagca
    10861 ggagttttca gtccagttat ttgagaaaat tggccgacgg atgtgcctta cccgcgaagg
    10921 aaaaaagttg ctgccgcaca tttatgaact tacccgagtg atggatacgc ttcgtgaggc
    10981 tgccaaaaag gagtcagatc cggacgggga actacgcgtt gtttcgggcg aaacgttgct
    11041 ctcttaccga atgccgcagg tgttgcagcg ttttcggcaa cgcgcaccaa aagtgcgttt
    11101 atcgttgcag tcgctgaact gttacgtgat ccgtgatgcg ctactgaatg atgaggccga
    11161 tgtcggcgtc ttttatcgcg tagggaatga tgatgccctg aaccgacgag agttgggtga
    11221 acaatcgctg gtgctggtgg cttcaccaca aattgtagat gtcgatttta ctgagccggg
    11281 aagacataac gcctgtagct ttattatcaa tgaaccacaa tgtgtcttcc ggcagatatt
    11341 tgagagcacg ctgcgtcagc ggcggatcac ggtggaaaac accattgagc ttataagtat
    11401 cgaaagcatc aagcgttgtg tcgcggcgaa tatcggagtc agctacctgc cgcgttttgc
    11461 ggtggcaaaa gagttggagt gcggagaatt aattgaattg ccttttggcg aacagtcgca
    11521 gaccattacg gccatgtgcg cacaccatgc tggaaaagcg gttagcccgg cgatgcacac
    11581 ttttattcag tgtgttgaag agagttttgt ggctggataa aaataatgtc gggattaaac
    11641 ccggcattta acttgtcgac aaagtgcgct ttattcatgc cggatgcggc gtgaacgcct
    11701 tatccggcct acaagatcgt gcaaattcaa tatattgcat gagccacgta ggcctgataa
    11761 gcgtagcgca tcaggcagtt ttgcgtttgc cagcactttt aataccaggc ttaaacccgg
    11821 cattgagatt actcttctgt cgtttgcggt ggcacgcccg ttccgcttgg tttacggcgc
    11881 ttaccaatat ttttggtgtc gcgatggcgt tttttcaccc gcggtttctc tttctcttta
    11941 gcttttttct tctcggcgcg ttttgccagt actttcttcg atggcttgcc ggtctgcttc
    12001 tcgcttggcg cacgcgtttt cgggcgtaac tcatcaataa cgcgagcttt aatcggctct
    12061 tcaatgtaac ggccaacctt gcccagcagc aaatggtcat gggcttccac cagcgaaatt
    12121 gcggtgcctt tacgaccggc gcgcgcggta cgtccgatac ggtgcaaata agtatcgcca
    12181 ctgcgcggca tatcgaagtt aaagacgtgg ctgacgtcag gaatgtcgat accgcgcgcg
    12241 gcaacatcgg ttgcaaccag tacgtttacg cgaccttcgg tcaaacgctt gatcgcttcg
    12301 ttacgcttgc cttgtaccat ctcaccttcg agatagcagt tgttgatgcc cgcttcgcgc
    12361 agccagtttg ccagctcatg cacacgttca cgcttacgca caaacacaat tgagcgggtc
    12421 gcttccggct gttttaacag atgcaccagc aatgcggttt tatgttccag gtcatcggcg
    12481 cggtaatacc actgatgaat ttttttgcgc tcacgggtgg acggattggc agaaacctcc
    12541 accggatctt ccagcagacg ctcggcaaag tcctgaatcg catcgccttc cagcgttgcc
    12601 gaaaagagca gggtctgttt acgccagcgc gtttcgccag caatatgttc gatatcctga
    12661 gcgaagccca tatccagcat acggtctgct tcgtcgagga tcagcgtttc aaccgcgcgg
    12721 caatcgaagt tctcttcttt tatgtattgc agcagacgtc cggtcgtggc gaccacgatg
    12781 tcctgatttt cgctgaacac ttccgcgtgg ttcatatagg ctacgccgcc ggtgatggtg
    12841 gcgatatcca gatgcgtatg tttcgccagt tcgcgggcat gatcggccac ctgcatcgcc
    12901 agctcgcgag ttggggtgag gatcaaaata cgcggcggac cggatttctt acgtgggaaa
    12961 tcgagcaggt gctgcaacgc tggcagcaga tacgccgccg ttttaccggt gcctgtcggc
    13021 gcagaaccga gtacatcacg gccatcgagc gcaggcggaa tggcggcagc ctgaatggcg
    13081 gtcgggcgag tgaaaccttt atcctggagg gcttccagca ggctttcgtc gagttcaagt
    13141 tcggaaaaag tcgttacagt catgttctac ctctgtgtgg ggcgctgatt atagacgtta
    13201 cggctgcaat cttcatctgt ttgtatggat atcgcttctc gggtattgtt ctcacccggt
    13261 gctatcgccg tttttcctgc aaggttttat tttcatgtca cagtctacat ccgtgcttcg
    13321 tcgtaatgga tttactttta aacagttttt tgttgctcac gatcgctgtg cgatgaaagt
    13381 gggaacggat ggcattttgc tgggcgcatg ggcaccggtg gctggggtga aacgttgcct
    13441 tgatatcggc gcgggtagcg ggttgctggc actgatgctg gcgcagcgaa ccagtgacag
    13501 cgtgataatt gatgccgttg agctggaaag tgaagctgcc gcgcaggcgc aggaaaatat
    13561 caaccagtcc ccgtgggcag agcggattaa cgtccatacg gcggatattc agcagtggct
    13621 cacacagcag acagtacgct tcgatttaat catcagtaac ccaccttact atcagcaggg
    13681 agtagagtgc gcgacacctc aacgggaaca ggctcgctat accactacgc ttgatcaccc
    13741 atcgctgctg acctgtgcgg cggagtgcat taccgaagag ggatttttct gcgtggtgct
    13801 gccagagcag gttggtaatg gttttactga actggcatta agcatgggct ggcatttacg
    13861 tttgcggacg gatgtggcgg aaaacgaagc gcgactgccg catcgggtgc tgctggcatt
    13921 ctccccgcag gcgggagaat gctttagcga tcgcttagtc attcgtgggc cagaccagaa
    13981 ctattccgaa gcgtatacgg cgctgaccca ggctttttat ctgtttatgt aatgattgcc
    14041 ggggggaagg atcgatggac cggaatgcgt gagcagttcc gggtaatcca gcgtaaaatg
    14101 caggccacga ctctccttgc gcatcatagc acagcgaaca atcaactcgg caacctgtac
    14161 cagattacgc agctccagca aattattcga cacgcggaag tgggcgtaat attcgtctat
    14221 ttcctgttgc aacatattga tacgccgcag ggcgcgctca agacgttttg ttgtgcgcac
    14281 aatgccgacg taatcccaca taaacagccg tagctcgtgc cagttatgct gaattactac
    14341 ccgttcgtca gggttctcaa cgcggctttc atcccacggt ggtaacgtac tgacgccgtg
    14401 ggcatcaggc atacgtctgg tgatatcttc tgccgccgac cagccataga ccagacactc
    14461 cagcaatgaa ttcgaggcca tgcggttagc gccgtgtaag ccggtataac tcacctcgcc
    14521 aatggcatac aagccctcga cgtccgtacg cccatgatca tcaaccatta caccaccgca
    14581 ggtatagtgt gcagcaggca caatcggtac cggttcttgt gtgagatcaa tccccagccc
    14641 gagcaatttt tcataaatca tcgggaaatg ctggcgaata aaatcggcgg gcttatggct
    14701 gatgtcgagg aacatacaat ctgcgccgag gcgtttcatt tcatggtcaa tggcgcgggc
    14761 gacaatatcg cgcggggcca gttcgccgcg ctcatcaaaa tcgggcataa aacgcgtgcc
    14821 atccgggcgc ttgagataag cgccttcgcc gcgcagtgct tccgttaaca ggaaattgcg
    14881 tgcctgtggg tgatacagtg cggtaggggg gaactgatta aattcgagat tggcaacccg
    14941 actgaggtgt actggcaata gcgga
//
LOCUS       DAJVRF010000100        14743 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJVRF010000100 DAJVRF010000000
VERSION     DAJVRF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14743
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.100"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            233..1897
                     /gene="tssM"
                     /locus_tag="OQN13_002805"
                     /pseudo
     CDS             233..1897
                     /gene="tssM"
                     /locus_tag="OQN13_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354198.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            1908..3260
                     /locus_tag="OQN13_002806"
     CDS             1908..3260
                     /locus_tag="OQN13_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HCS1616114.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQHTRKRLEPQPKSNAENAAQTTVRTLIYAPETLASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPIESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            3284..3766
                     /locus_tag="OQN13_002807"
     CDS             3284..3766
                     /locus_tag="OQN13_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCS1616115.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            3810..4753
                     /locus_tag="OQN13_002808"
                     /pseudo
     CDS             3810..4753
                     /locus_tag="OQN13_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4763..5242
                     /locus_tag="OQN13_002809"
     CDS             4763..5242
                     /locus_tag="OQN13_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616116.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNIHVILLTIIKLFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(5379..6164)
                     /locus_tag="OQN13_002810"
     CDS             complement(5379..6164)
                     /locus_tag="OQN13_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616117.1"
                     /translation="MNLRRVCCICVLFSLLAGCVSESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(6494..6570)
                     /locus_tag="OQN13_002811"
     tRNA            complement(6494..6570)
                     /locus_tag="OQN13_002811"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6534..6536),aa:Asp,seq:gtc)
     gene            complement(6703..7434)
                     /gene="dnaQ"
                     /locus_tag="OQN13_002812"
     CDS             complement(6703..7434)
                     /gene="dnaQ"
                     /locus_tag="OQN13_002812"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCS1616118.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            7499..7966
                     /gene="rnhA"
                     /locus_tag="OQN13_002813"
     CDS             7499..7966
                     /gene="rnhA"
                     /locus_tag="OQN13_002813"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCS1616119.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELTAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(7963..8685)
                     /locus_tag="OQN13_002814"
     CDS             complement(7963..8685)
                     /locus_tag="OQN13_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1616120.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            8719..9474
                     /gene="gloB"
                     /locus_tag="OQN13_002815"
     CDS             8719..9474
                     /gene="gloB"
                     /locus_tag="OQN13_002815"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCS1616121.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            9546..10904
                     /gene="mltD"
                     /locus_tag="OQN13_002816"
     CDS             9546..10904
                     /gene="mltD"
                     /locus_tag="OQN13_002816"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCS1616122.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(10953..11549)
                     /locus_tag="OQN13_002817"
                     /pseudo
     CDS             complement(10953..11549)
                     /locus_tag="OQN13_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(11580..12380)
                     /locus_tag="OQN13_002818"
     CDS             complement(11580..12380)
                     /locus_tag="OQN13_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCS1616123.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            12621..13535
                     /locus_tag="OQN13_002819"
     CDS             12621..13535
                     /locus_tag="OQN13_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1616124.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(13532..14335)
                     /gene="dkgB"
                     /locus_tag="OQN13_002820"
     CDS             complement(13532..14335)
                     /gene="dkgB"
                     /locus_tag="OQN13_002820"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCS1616125.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(14498..14574)
                     /locus_tag="OQN13_002821"
     tRNA            complement(14498..14574)
                     /locus_tag="OQN13_002821"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14538..14540),aa:Asp,seq:gtc)
BASE COUNT         4062 a         3481 c         3498 g         3702 t
ORIGIN      
        1 ggaacagacc tatctgcaac tgctggagca acgttacctg ccctcgctgt ttaacgggct
       61 ggtcaaagcg atgaacgccg cgccgccgga gagtgaagaa aaactcgcgg tgctgcgcgt
      121 gatgcgaatg ctggaggaca aaagcggacg taacaatcag gtggtgaagc agtatatggc
      181 aaaacgctgg agcgaaaagt tccacggtca gcgcgatatc caggcacaac tgatgtccca
      241 tcttgactac gcgctggctc atactgactg gcacgcagag cgtcaggcgg gcgacggtga
      301 cgccatcagc cgctggacgc catatgacaa gcccgtggta tcagcacaga aagaactgag
      361 caaactgcct gtctaccagc gggtttacca gagcctgaaa acgcgggcgc tgggcgttct
      421 tcctgccgac ctcaatctgc gtgaccaggt agggccaacc tttgaccagg tgtttacatc
      481 tgccgatgac aacaaactgg ttgttccaca gtttatcacc cgctacggcc tgcaaagcta
      541 ttttgtaaaa cagcgcgatg aactggttga actgacagcg atggattcct gggtacttaa
      601 cctcacccgc aatgtgaaat acagtgacgc cgaccgtgcg gaaatccagc accagttgac
      661 cgagcagtat atcagcgact acaccgccac ctggcgggct gggatggaca atctgaatat
      721 ccgcaatttt gagtccatcg gacaactgac cggggcgctg gagcaggtta tcagcggcga
      781 ccagcctttg cagcgggcgc tgaccgtgct gcgtgacagc acacagccag gcgtcttttc
      841 tgaaaaactc tctgccaaag aacgggagga agccctggca gagccggatt accagttact
      901 cacccgcctc gggcatgaat tcgccccgga aaacagtacc ctggcagtac agaaagacaa
      961 agaaagcacg atgcaggccg tgtatcagca actcaccgag ttgcaccgct acctgctggc
     1021 aatccagaac gcgcctgtac cagggaaatc ggcgctgaaa gccgtgcagt tacggcttga
     1081 tcagaacagc agcgatccga tattcgccac ccgccagatg gcaaaaacgc tgcctgctcc
     1141 gctcaaccgc tgggttggca gactgactga ctaggcctgg catgtggtga tggtggaggc
     1201 tgttcattat atggaagtgg actggcgcga cagcgtggtg aaaccgttta acgagcaact
     1261 ggcaaataac tatccgttta atccgcgttc tgcacaggat gcctcactgg atgccttcga
     1321 acgcttcttt aaaccggatg gcatactgga taccttctac cagcagaacc tgaagctgtt
     1381 tatcgataat gacctgagtc tggaggatgg cgataacaac gtcattattc gcgaagatat
     1441 tattgcgcaa ctggaaactg cgcagaaaat ccgtgacatc ttcttcagca aacagaacgg
     1501 tctgggaaca tcctttgccg tggaaacggt atcgctttca ggcaataaac gccgcagtgt
     1561 actgaacctt gacagtcagt tagtcgatta cagccagggc cgtaactata ccgcccatct
     1621 ggtctggcct aacaacatgc gcgaaggcaa cgaaagtaag ctgacgctca tcggcaccag
     1681 cggcaacgcg ccgcgcagta tcagcttcag cgggccgtgg gcgcagttcc gcctgttctg
     1741 ggccggacaa ctgaccggag tacaggatgg caactttacc gtgcgcttta gcgtggacgg
     1801 tggcgcgatg acctaccgtg tgcataccga cacggaagat aacccgttca gcggtgggtt
     1861 gttcagccag tttggtctgt cagacacact gtactgagga ggcagggatg aatagtaacg
     1921 tactgacaca aactatcgtt accggcagtg acccgcgcgg gctgccggaa ttcagcgcca
     1981 tccgcgagga aataaacaaa gccagccacc cgtcacagcc tgagctgaac tggaaactgg
     2041 tggagtcgct ggcgctggcg atttttaaag ccaacggtgt ggatttacac accgccacct
     2101 actatacgct tgcccggaca cggacacagg gactggcggg attctgcgaa ggtgcggaac
     2161 tgctggcggc aatggtaagc cacgactggg ataagttctg gccgcagggc ggcccggcgc
     2221 gtactgaaat gctggactgg tttaactccc gaaccggcaa tattctgcgt cagcaaatct
     2281 cctttgcgga atccgaccta ccactgatct accgcacaga gcgggcattg cagcttatct
     2341 gcgataagct ccagcaggtg gaactgaagc gcgttccgcg cgtggagaat ctgctctatt
     2401 ttatgcagca cacgcgtaaa cggcttgaac cccagccgaa gagtaacgct gagaacgccg
     2461 cacagaccac ggtcagaacg ctgatttatg ccccggaaac actggcatct tccacaccag
     2521 aagcggtagt gcctcccctg cccggcctgc ctgagatgaa agtggaagtg cgcagtctga
     2581 cagagaatcc cccacaggcc agtgtgataa agcaaggcag tacggtaaga gggtttatcg
     2641 cagggatcgc ctgttcagtg gctgtcgcct cagcattgtg gtggtggcag gtctatccgg
     2701 tgcagcagca actgttacag gttaacgaca ccgctcaggg cgcagcaacg gtgtggatgg
     2761 cctcacctga actcgaaaac tatgagcgca ggctgcaaca acttcttgat acctccccgg
     2821 tacagccgct ggaaaccggg atgcagatga tgcgtgttgc cgacagtcgc tggccggaaa
     2881 gcctgcaaca gcaacaggcc tcgacacaat ggaatgaggc actcaaaacc cgcgcacaga
     2941 gtagcccgca gttgcgtggc tggttgcaga cccgccagga cttacatgct tttgcagatc
     3001 tagtgatgca gcgcgagaaa gagggactaa ccctttccta tatcaaaaat gtcatctggc
     3061 aggcggagcg gggactgggg caggaaaccc ccattgagtc tctgttgacg cagtaccatg
     3121 atgcccgtgc gcagaagcag aatacagatg cgctggaaaa acaaattaat gagcgactcg
     3181 aaggcgtgtt aagccgctgg ctgctgctga agaataacgt catgccagag gcggcaaccg
     3241 gtaccacggc cgaaaaataa ttatttaatt aaaggagatt atcatggcaa atataagtta
     3301 tttatctttg agtggagaga cacaagggct aatttcagca ggttgctcaa cgcttgattc
     3361 tgtcggaaac aaagcgcagc cagaacataa agatcagatc atggtttatg cgttaatgca
     3421 ttccatctca agaagtcaaa atgtcaatca tcatgaatta attatcacca aaccggttga
     3481 taaatcctca ccattgctgg caaaagcact tagtgacaat gaaaaaatgg caatatgtga
     3541 atttatactc taccgaacca gtaaagcggg tatttaccag ccttattata aaataaatct
     3601 gtcaaaagcc cgaatatcaa gtattgattt tgtcacaccg catgctgtcc tggaaaaaga
     3661 actggaacca caggagagaa ttgcctttat ttatgaagat atttcctggg agcatacgtt
     3721 agccggtacg aatgcaatga gtaaatggca ggatcgtgta cagtaactta ctgtaataac
     3781 ataataaatt actattgcgg agagataaaa tggctggttt aaggaataac aacaacacac
     3841 agaatgcaca gtgggctgat tatgtgggtg acattcttcg gggggctcag ccgataaacc
     3901 agctagtacc tcagcaccct tatcttaacg acgtcccact cattgatgag ttaaggcatc
     3961 agaacactca tcatgttatt ctcttgacac ttgatgttgc aaaaaaaaaa aaaaaaaaaa
     4021 aaaaaaaaaa aaaaaaatac tcagcccaat aacatcattt gattacattc attttatcac
     4081 tacacatcct tcaggtataa aagatacact cgcctggtta gttaacgcga gtaaattaat
     4141 gactgagttt gatgacaatg ggaaaattat atttaacatt aatgccttaa agtatacaaa
     4201 agcaagttac tttgaaatac ttggagagaa atatataaaa atcacaacat ccagcccctg
     4261 gcttttagaa aagctcggta aatatatttt ctcttcacga gcgcctcaag ttctggaact
     4321 cgctattggc tggcgaggag ctttatctga atcaataaaa ggagttaagt tctgtatctg
     4381 gttttcagta gcctggagaa ccatagaatt tattatgagt tcagagcgag atctggttaa
     4441 ttttcttggc gatttttcaa tggatgtggc caaagcagtt atagccggtg gtgttgcaac
     4501 cgctattgga agcctggctt cttttgcctg tgttagcttt ggctttccag taattcttgt
     4561 cggaggagca attttactga cagggatagt gtgtacagtt gttttaaatg aaatcgatgc
     4621 tcaatgccat ttatcagaaa aattaaaata tgcaattaga gatggactaa aacggcaaca
     4681 ggaacttgat aaatggaaaa gggaaaacat gactccattt atgtatgttc ttaacactcc
     4741 acccgtgata tgaggattaa ccatgcgttt acatgttaaa ttaaaagagt ttctttcgat
     4801 gttttttatg gccatattat ttttccctgc attcaatgct tctttgtttt ttaccggagt
     4861 taaacctcta tattcaataa taaaatgctc cacggaaata ttttatgatt ggagaatgtt
     4921 gattctgtgc tttggtttca tgtcgttttc ctttttaaat attcatgtaa ttttacttac
     4981 gataataaaa ttattcttaa tcaaaaaaac aaaggtagtc aatttcgcca cagacataac
     5041 aatacagcta actcttatat ttttactaat agctatcgta atagcacctt taatagctcc
     5101 ttttgtcaca ggatatgtca atgctaatta tcacccctgc ggtaataata cgggaatttt
     5161 cccaggagca atctatatta aaaatggcat gaaatgtaat aatgaatata tttctcgcaa
     5221 ggaagattca gcagttaaat aattaaacat tctaaaacaa acagttttta actccggatt
     5281 ccatgtcctt tcttgcactg tctttgtcaa gaaaaatttc ttatgcaaag aattttgtat
     5341 tatttccgcc agaaattcct ggcggcagat ataatttatt attgatagcg gttaaccaaa
     5401 tcttttacca tcgctttata ggtcttttgc ttaagttgca tgatttgctg ctctgtcata
     5461 tcagtcgtac tggcttcatt cttgtcgggc aatggtggaa gcactttata ttcataattc
     5521 accggcgacc aggcagccca ctcacctgta gctacatcca ccagcgtgaa tcgtaccaga
     5581 tagcgcagtg acatcgtgtc agtcaatttt tgatttttat aatctgacca aactattgat
     5641 ttcaatccag agtcatattt tcctgtctgc aacgtatcct ggaaaacaat aatcgctttc
     5701 tgatgccctt ttgcagccac aaaacgcagt gcctgcatcc agttcatatt ctcagcactg
     5761 gcaacatcct cgttctcatt tttatctttg tttttgttac aagtcagagg cttttgcctg
     5821 tccgggatac cagagaatgt ggaaacagta taatattttc gcatctcctc ctgcataatg
     5881 gtttccggtg cgcggttgcc ggattgaaca agtattacag gagaattaag gggaacgcgg
     5941 aactcatctc catcaagcgc agcttgaata tcctcttcgg atacggccag agtggtttca
     6001 ttaccgaata aatctttgtc tgtcagttgc tggggagtgt ttttattaat attactttgt
     6061 gtgacttgag cttttttctt cttttcatca atagaagatt cagagacaca tcctgcaagc
     6121 agcgagaata atacacatat acaacaaacc cttcttaaat tcataatcgg acactccctc
     6181 gcctgtcata atcatggtaa tgtttagcta acgattttta attatatact ccacttgaat
     6241 cagaacaaac aaaaataaca gtatatataa attcattaaa tcaatagaaa aatgaaaatc
     6301 ataacattca aatatattta aattgaatga tttgatagta tgattaaata ttaatgatat
     6361 ggtctaccat atgtgattaa cacatttaca gcataacaaa accatagacc gaagaaatag
     6421 cgggatttaa atgcaaaaaa accgcaaaat taaaaatctt gcggttctct gaactcattt
     6481 tcatgagtga atttggcgga acggacggga ctcgaacccg cgcccccctg cgtgacaggc
     6541 aggtattcta accgactgaa ctaccgctcc gcgttgtgtt ccgttgggaa cgaggcgaat
     6601 attacggatt gcctcaccct tcgtcaacgg tttttctcat cttttgaatc gtttgctgca
     6661 aaaatcgccc aagccgctat ttttagcgcc ttttacaggt atttatgccc gccagaggca
     6721 gcttccgccc ttcttctcca ccagatcaag acgggcttca tgagctgcaa gctcttcatc
     6781 tgtcgcaaaa acaacgcgta acttacttgc ctgacgtaca atgcgctgaa ttgttgcttc
     6841 accttgttgc tgctgtgtct ctccttccat cgcaaaagcc atcgacgttt gaccaccggt
     6901 catcgccaga taaacttccg caaggatctg tgcatcgagt aatgccccgt gtagcgttcg
     6961 tttactgtta tctatttcgt agcgggcaca taacgcatcg aggctgttgc gcttaccagg
     7021 aaacattttc ctcgccaccg caaggctatc ggtgacctta cagaaggtat tggtcttcgg
     7081 aatatcgcgc ttaagcaacg aaaactcgta gtccataaag ccgatatcga acgctgcgtt
     7141 atggatcacc aactccgcgc cgcggatata gtccatgaac tcatcggcta cttcggcaaa
     7201 cgtgggctta tcgagcaaaa attcatcggc aataccatgt acgccaaagg cttccggatc
     7261 caccagccga tcgggtttga gataaacatg gaagttattg cccgtcaggc gacggttcac
     7321 cacttcaacg gcaccaatct caatgatctt gtggccttca tagtgcgcac caatctggtt
     7381 cataccggtg gtttcggtat cgagaacgat ctggcgtgta attgcagtgc tcatagcggt
     7441 catttatgtc agacttatcg ttttacagtt cgattcaatt acaggaagtc taccagagat
     7501 gcttaaacag gtagaaattt tcaccgatgg ttcgtgtctg ggcaatccag gacctggggg
     7561 ttacggcgct attttacgct atcgcggacg cgagaaaacc tttagcgcag gctacacccg
     7621 caccaccaac aaccgtatgg agttgacggc cgctattgtc gcgctggagg cgttaaaaga
     7681 acattgcgaa gtcattttga gtaccgacag ccagtatgtc cgccagggta tcacccagtg
     7741 gatccataac tggaaaaaac gtggctggaa aaccgcagac aaaaaaccag taaaaaatgt
     7801 cgatctctgg caacgtcttg atgctgccct agggcaacat caaatcaaat gggaatgggt
     7861 taaaggccat gccggacacc cggaaaacga acgctgtgat gaactggctc gtgctgcggc
     7921 aatgaatccc acactggaag atacgggcta ccaagtcgaa gtttaagctt gtggtttacg
     7981 acattgccgg gtggctccaa ccgcctggcg aattcgtggc ttgtttttac tctgtttcat
     8041 cgggtttagc gttaaaggaa tagtccgttt ccgggcaaca ataagttgta agcaaccaag
     8101 cgcaggaata tgcgcattca atagttttcc tccgtgtttg ttccacggga gaacgtggaa
     8161 acggctggcg tgcagcactt caaaattcaa caaagagagc cagtccagtt gccgcatcag
     8221 agtaaacatc cggctgttat agggcgaggt ttttcgcaat accggcacaa gtttgcgtaa
     8281 tcccattaaa ctaatgggat tgaagccact aatcaccagc cagccatcat caatcaatac
     8341 ccgatcggtt tcacgcaata aacgatgcgg atcggtgcac cacggcaatg tatgtgccag
     8401 tagacaaaca tcaacggatt tatcggcaaa aggaagatga agtggatccg cctgcacctg
     8461 gacgggcatt ccttgcgcag aaacattcac ttgatgagaa accgcgcacg cttcgcaatt
     8521 gatttctgcg cttaaattgc caatcttaag cagatgaaaa ccatacattt tagtgaacca
     8581 cgggttgagc tggcgctcca gcgccttgcg ataaagctct ccccagggca aatcgcccca
     8641 gcgatcagga gccacgacag tttgagggac tcttgccggt ttcatcacaa ccttccgttt
     8701 cacactaaga ggtaatctat gaatcttaac agtattcccg cctttgatga caattacatc
     8761 tgggttttga atgatgaagc aggtcgctgc ctgattgtcg atcccggaga cgcagagcca
     8821 gtattaaacg ccattgccgc caataactgg caaccggagg ccatatttct cacccaccat
     8881 catcacgatc acgttggcgg cgtaaaagaa ctggtggaaa agtttccgca aattgtggtg
     8941 tatggtccac aagagacaca agataaggga acaacacagg tagtcaaaga tggcgaaact
     9001 gccttcgttt tggggcatga atttagtgta attgccacac cgggtcacac tttaggacat
     9061 atctgttact tcagtaaacc ttatctattt tgcggcgaca cgctgttttc tggtgggtgt
     9121 ggtaggttgt ttgaagggac agcatcacaa atgtatcaat cacttaataa gttaagtgcg
     9181 ttacctgacg atacattggt atgttgtgct catgaatata cgttatcaaa tatgaagttt
     9241 gctttgagta ttcttccgca cgatttgtcc ataaatgatt attatcgtaa agttaaggag
     9301 ttacgggcaa aaaatcaaat aacactaccc gtaattctga aaaatgagcg gcaaattaat
     9361 gtttttttaa gaacggaaga tattgattta attaatgtaa ttaatgaaga aacattattg
     9421 caacaacctg aagagcgttt tgcatggtta aggtcaaaga aagataggtt ctgataaaac
     9481 tttcttgtca tcggctccgt tcgccgttat gatcggtcgt cttttaagca actattgaca
     9541 cacacatgaa ggcaaaagcg atattactcg cctctgtcct gctcgtgggt tgccagagta
     9601 ccggcaacgt tcaacagcac gcacagagcc tttctgcagc tggtcaaggg gaagcagcaa
     9661 agtttacaag tcaggcacga tggatggacg atgggacgtc tatcgcgcca gatggtgact
     9721 tgtgggcttt cattggcgac gagctaaaga tgggaattcc ggaaaatgac cggattcgcg
     9781 aacagaaaca gaaatattta cgcaataaga gctatctcca cgatgtaact ttacgggcag
     9841 agccgtatat gtactggata gcagggcaag ttaaaaaacg taacatgcct atggaactgg
     9901 tactactacc catagtggag agcgcttttg atcctcacgc aacgtctggc gccaatgccg
     9961 caggcatctg gcagatcatt ccgagcacgg ggcgcaatta tggtttgaaa cagacccgca
    10021 attatgacgc gcgtcgcgat gttgttgctt caacaactgc cgcgctgaat atgatgcagc
    10081 gtctgaacaa aatgtttgac ggcgactggc ttctgaccgt agcggcttat aacagcggcg
    10141 aaggtcgggt catgaaggca attaaaacga acaaagcgcg tgggaaatcc acggacttct
    10201 ggtcgttacc gttgccgcag gaaacgaagc agtacgtgcc taaaatgctg gcattgagtg
    10261 atattctcaa aaacagcaag cgttatggcg tacgtctgcc aacgaccgat gaaagccgtg
    10321 ctctggcgcg tgtgcacctg agtagcccgg ttgaaatggc gaaggttgca gatatggcgg
    10381 ggatttccgt cagcaagctg aagacattca acgctggcgt gaaaggctcc acgctgggcg
    10441 caagtgggcc gcagtacgtg atggtgccaa agaagcatgc agatcaactg cgtgaatctc
    10501 tcgcttcagg cgaaattgct gctgtacagt cgacgctggt tgccgacaat acgccgctta
    10561 acagccgtgt ttacaccgta cgctctggcg acacgctttc aagcatcgct tcacgtctcg
    10621 gcgtaagcac caaagatttg cagcagtgga acaaactgcg cggatctaag ctgaagccag
    10681 gccaaagttt gacgattggt gcaggcagta gcgcacagcg gttggcaaac aacagcgata
    10741 gcattacgta tcgtgtgcgc aaaggcgatt cgctttcaag cattgctaaa cgccacggcg
    10801 tgaacatcaa agatgtgatg cgctggaaca gcgacactgc gaatctgcaa ccaggcgata
    10861 agctgacgtt gtttgtgaaa aacaacaaca tgccagattc ctgacaaacc agataataaa
    10921 aaaggcaccg attcccccgg tgcctttttt atttatgccg ctttatgtgc ttctaccatg
    10981 atgatatcac tggtgaaaga gccatcagtc tgcaaggaaa aatacgttct cacctctgtc
    11041 gatgcgctct gttggtaaat gcgaatagcg tctactaacg cttctggcgt acgcattctc
    11101 acgacccatg aagaaaattc cagcggtaac ttatctgtaa ttaaattatc aactatcaga
    11161 ttggcttcat tgattaacgt caaccacgca ccgctggcgt agtttcgtac gtgagaggta
    11221 tcgcgtaatg cttctaccgc ctgtaaccag atgtcgcgca ctgggtgacc cggagacatt
    11281 acatccatca caatcagcct accgccaggt ttcaatatcc tattcacttc tcgcagtgct
    11341 gcaccaacat catgccagtg atgggcagaa taacggctga taacaatatc aaatgcgtta
    11401 tcggcaaatg gcagacttgc ggcatatccc tggcgggtgg tgatattttt cagttgccgg
    11461 gcttcggcag cttgtgccac gacatccagc atgtgggcag ataagtcata cgccaccacc
    11521 gcgctcacgt tttgcgcagc gccaaacgtt ggtctgccct gcggcagacc tgacatacct
    11581 tatttatcag gcttgccggg actgaattca acgagtagcg gattgtgatc ggaagcgcgc
    11641 gtaaccagta cagaagcttc actgacgttc agaccacggt agaaaacaaa atcgagcggg
    11701 cgaccaaacg cccgacggcg ctgatcatcg gtaaaacgca cctggcgcag cgacatttcc
    11761 cgcgcaaagc gatataacgc gttcatcctt ctacggctcc aggcattgaa atctcccgcc
    11821 ataatgaccg ggccgctgtg gtgagctatc tgatcaccaa taggaagtaa ctgcttacta
    11881 tagacatcca cgcccagact gaagttgacg gcgtgtatat taaccaccat caacaggcgg
    11941 gtgtcaggta atggatagac cgtcaccagt gccgacttcg ccagacgcaa aatgggttct
    12001 cgttcacgta acgggcagca atacactgga tgtgccgccg aaagggtcat tacgccagaa
    12061 ggatgttgtg gcagcacgaa agcgggtacc tgatcggcgg caagatagtt agcggtcgca
    12121 aactgtacca actctggcgt tgtttgcgct tcctgcaata acaccagatg tgcgtctttg
    12181 ccgtaattct ttaataccga caaccattca gcgcgttgct gtttgtatat gttccacacc
    12241 aggatccgaa tacgctcttc ggtacttaac ggttctccgg gtggtaatgc ctggccgatg
    12301 ctcgcaaaag accccggcgg taagatcctc tccgcaggtt gtccggcaac atagcgcatg
    12361 gcataggtgt tttttcgcac ttttgacttt gaaacctcta ttacgtcaac ccttccgcac
    12421 tcgggagggt tctactgtta tagggatttt agctcactct ttcaacgagc aattgtatat
    12481 tgttatgtaa gcaagtgctt acaccggtca gaaatttgct cattattgag aacattaaat
    12541 cggatgacat atcacgcatc ctgatggttt aatgacaaat aaattcaaaa tatctcatct
    12601 attttgcaac aggagcaaaa atgaaagcca cgtcggaaga actcgccatt tttgtttcgg
    12661 tcgtcgaaag cggcagcttt agccgggcag cggaacaatt agggcaagca aactcagcgg
    12721 taagccgggc ggtgaaaaag ctggaaatga aacttggcgt tagcctgctt aatcggacca
    12781 cgcggcaact tagcctgacg gaagaaggcg agcgttattt tcgtcgcgta cagtcaattt
    12841 tgcaggagat ggcagcggca gaatcagaaa ttatggagac gcgtaataca ccgcgtggac
    12901 tgttacggat cgatgccgca actccagtgg tgctgcactt tctgatgccg ttaattaagc
    12961 ctttccgtga acgctatccg gaagtcactt tatcgctagt ctcctccgaa acgattatta
    13021 atttgatcga aagaaaagtg gatgtcgcga tacgcgctgg tacgttaacg gactccagct
    13081 tacgtgccag gccgttattt aacagttatc gaaaaattat cgcctccccc gattatattt
    13141 cccgctacgg gaagccagaa acgatcgacg atttaaagca acatgtttgc ctgggattca
    13201 ctgaacccgc ttccctcaat acctggccga tagcctgtag tgatggacaa ttacatgagg
    13261 tgaagtacgg tttgtcatcc aatagtgggg aaacactgaa acagctttgc ctgagtggca
    13321 acgggattgc gtgtttgtcc gactacatga tcgacaaaga aatcgctcgc ggagaattgg
    13381 tggagttaat ggcagataaa gtgttgccag tggaaatgcc attcagtgcc gtctattaca
    13441 gcgaccgtgc ggtaagtacg cgcatccggg cttttatcga tttccttagc gagcatgtaa
    13501 aaacagctcc cggaggagct gtcagagagg cttaatccca ttcaggagcc agaccttccg
    13561 ggctaaccag gcggtcgttg caatccagtg cggcgatcgc ttttttatct tcggcatcaa
    13621 gctgtaaatt ttgtgcctta agattacttt ccaggttttt acgtttagta gaagaaggaa
    13681 ttactgagta accttccccc atagcccacg ccagaatcac ttgtgccgga gtcgcattgt
    13741 gtttagctgc aatacgagca ataacctcat ctttcagtgc cttaccataa gccagcgtca
    13801 tataggaagt gatatgaata ccgtgctgtt tagcccaggc aaccacttta cggttttgca
    13861 gataaggaga gagttcaatc tggttggtag cgatgttttc agcgccaaca gcagcaatcg
    13921 ctttttccat caacgggatc gtgaagttgg aaataccgat ctcacgtgtc agaccttgtt
    13981 ttttggcctc cagcagcgcc tgcataaact cttcaacaga gacttcatcg tttggtgacg
    14041 gccagtggat tagcgtcaga tcaacataat cggtacgcaa tttttgcagg ctctctttta
    14101 gactcgggat caatttgtct ttgctgagat tttcaatcca gattttagtg gtgatgtaga
    14161 gttcatgacg tggcacgcca ctttctgcaa tcgcctgacc tactgcggct tcgttatcat
    14221 agatttgtgc agtatcaatt gcgcgataac caagttcaag cgccgttttc acagatgaaa
    14281 taacaacgtc gtctttgaga cggaaagtac ctaaaccaaa tgcagggata gccataatat
    14341 gcctctttta atgctatttt cgttaactga ggtgattatg ggcttcagac tgttgcggaa
    14401 aaagagcata aaatgcagag gatttttgcg attctggcaa taatagatat acagacgaaa
    14461 aaaaacctcg agcgttaact cgaggcttct taataagtgg cggaacggac gggactcgaa
    14521 cccgcgaccc cctgcgtgac aggcaggtat tctaaccgac tgaactaccg ctccaccgaa
    14581 ttcttttaca accaccggtt ttatgaccgg cttactgctt aatttgatgc ctggcagttt
    14641 atgaatcact tcgggctgtt gcttcgcaac gttcaaaagc tttcgctttt gtcctactct
    14701 cttatcgagt agtgaactgc ctcaaaggtc agtgccattc ttc
//
LOCUS       DAJVRF010000101        14404 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJVRF010000101 DAJVRF010000000
VERSION     DAJVRF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.101"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OQN13_002822"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OQN13_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCS1616126.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OQN13_002823"
     CDS             1828..3147
                     /locus_tag="OQN13_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCS1616127.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3519..4901
                     /gene="dosC"
                     /locus_tag="OQN13_002824"
     CDS             3519..4901
                     /gene="dosC"
                     /locus_tag="OQN13_002824"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCS1616128.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            4926..7325
                     /gene="dosP"
                     /locus_tag="OQN13_002825"
                     /gene_synonym="pdeO"
     CDS             4926..7325
                     /gene="dosP"
                     /locus_tag="OQN13_002825"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCS1616129.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7584..8165
                     /gene="ddpX"
                     /locus_tag="OQN13_002826"
     CDS             7584..8165
                     /gene="ddpX"
                     /locus_tag="OQN13_002826"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCS1616130.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8179..9729
                     /locus_tag="OQN13_002827"
     CDS             8179..9729
                     /locus_tag="OQN13_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1616131.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSGQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            9731..10753
                     /locus_tag="OQN13_002828"
     CDS             9731..10753
                     /locus_tag="OQN13_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1616132.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            10750..11646
                     /locus_tag="OQN13_002829"
     CDS             10750..11646
                     /locus_tag="OQN13_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCS1616133.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11643..12629
                     /locus_tag="OQN13_002830"
     CDS             11643..12629
                     /locus_tag="OQN13_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1616134.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            12658..13548
                     /locus_tag="OQN13_002831"
     CDS             12658..13548
                     /locus_tag="OQN13_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1616135.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQPDVIVLDEPTSA
                     LDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQQV
                     LTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHGCE
                     VRQSLAIREDGRELRCWRAL"
     gene            complement(13604..14035)
                     /gene="osmC"
                     /locus_tag="OQN13_002832"
     CDS             complement(13604..14035)
                     /gene="osmC"
                     /locus_tag="OQN13_002832"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCS1616136.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3577 a         3558 c         3629 g         3640 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gcttttttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg gggccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggca cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttatctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcaaagacc ttcttccctg acttctctaa agtgggcacc ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcatc cgcaacccac gtcaatgaaa
      841 tgagcaaccc agggcgcgac tatccgttgg ctatgttact gctgatggta gcggcaatct
      901 gcttaagctc tgttggtggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcacagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcta ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ctctaccgat atgtatgttg
     1501 aattactggt cgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgctcgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcatatatg gatatctgct cccgaaacga gaaattagcg
     1861 attagaagac cagcgatact ggttgcactg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggtgt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taaccccacc agccgggccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct gcaacgtctc ggtataaaca cggtcttttt ccaggttaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccat ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattta tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgca gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgag tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgca aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaagt atcgcacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ctaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact cccatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtccta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtca atctcgcacg ttgcgttttg
     3481 attccgtctg atttcccagc cttataaggg tgtgaattat ggagatgtat tttaaaagaa
     3541 tgaaagatga gtggaccgga cttgtcgaac aggcagatcc actcattcgt gctaaagccg
     3601 cggaaattgc cgttgcgcat gctcattatc tgagtatcga gttttatcga attgtccgca
     3661 tcgacccgca tgccgaagaa ttcttgagta atgaacaagt tgagcggcag ttgaaaagtg
     3721 cgatggaacg ctggattatt aacgtgcttt ctgcccaggt tgacgatgtc gaaaggctaa
     3781 tacaaatcca gcataccgtc gcggaagtgc atgcccgcat aggaattccg gtagaaattg
     3841 tcgagatggg gtttcgggtg ctgaaaaaaa tcctctatcc ggtaatcttc tcttcggatt
     3901 attccgccgc agaaaaactt caggtctatc atttctcgat taacagtatt gatatcgcga
     3961 tggaagtgat gacccgcgcg tttaccttta gtgacagtag tgcctcaaag gaagatgaaa
     4021 attatcgtat cttctcgtta ctggaaaacg ccgaagaaga aaaagaacgg caaatagcct
     4081 caatactttc atgggaaata gatattatct ataaaatcct gctggattct gatttaggca
     4141 gtagtttgcc attaagccag gctgattttg gcctgtggtt taaccataaa ggtcgacatt
     4201 attttagtgg cattgccgaa gtaggccata tctcccgtct cattcaggat ttcgacggta
     4261 ttttcaatca aaccatgcgt aacaccagga atttgaataa cagaagtctg cgggtgaaat
     4321 ttttattaca gataagaaat accgtatcgc agattattac cttgctgcgt gaattgtttg
     4381 aagaagtatc gcgccacgaa gtcggtatgg atgtactaac gaaattactt aaccgccgtt
     4441 tcctgccgac tatcttcaaa cgcgaaattg cccatgccaa ccggaccggt acaccgctgt
     4501 cagtgctgat tattgacgtt gataaattca aagagatcaa cgatacgtgg ggccataaca
     4561 ctggcgatga aattctgcgt aaagtctctc aggcctttta tgacaacgtc cacagtagtg
     4621 attatgtttt tcgctacggg ggcgatgaat ttatcattgt tttgactgaa gcttctgaaa
     4681 acgaaacgtt acgtaccgca gaacgtattc gcagtcgggt ggagaaaacc aaactgaaag
     4741 ccgcaaacgg cgaggatatt gccctctcac tttccatcgg tgccgccatg tttaatggtc
     4801 atcctgacta tgagcgcctc attcaaatag ccgatgaagc tctgtatatc gccaaaagac
     4861 gaggtagaaa ccgtgttgaa ctctggaaag ccagtcttta gatgcgccag gatgcagagg
     4921 taatcatgaa gctaaccgat gcggaaaatg ccgccgatgg catttttttc cccgcccttg
     4981 aacaaaatat gatgggcgcg gtgttaatta acgaaaatga tgaagtgatg tttttcaacc
     5041 ccgccgcaga gaagctctgg gggtacaaac gtgaagaagt cattggcaat aacattgata
     5101 tgctgattcc acgggatttg cgtcctgcgc atcctgaata cattcgtcat aaccgtgaag
     5161 gtggtaaagc gcgcgtcgag gggatgagtc gggagctgca gctggagaaa aaagacggca
     5221 gtaaaatctg gacccgtttt gcgctatcga aagtgagcgc cgaggggaaa gtttattacc
     5281 tggcgctggt acgggatgcc agcgtagaaa tggcgcaaaa agaacagacc cgacaattga
     5341 ttattgccgt tgaccatctc gaccgaccgg tgattgtcct cgatccggaa cgccatattg
     5401 tgcagtgcaa tcgcgcattt accgaaatgt ttggttactg cattagcgaa gccagcggta
     5461 tgcagcccga tacactcctg aacattcctg aattccctgc cgataaccgc attcgtttac
     5521 aacagttgct atggaaaacc gcccgcgatc aggacgaatt tctgctgttg acacgcaccg
     5581 gtgaaaaaat ctggattaaa gcctctatca gcccggttta tgacgtgctc gcgcatctgc
     5641 agaacctggt aatgaccttc tcggatatca ccgaagaacg gcagatccgc cagcttgaag
     5701 gcaatattct cgccgccatg tgcagcagcc cgccatttca tgaaatgggg gaaatcattt
     5761 gtcgtaacat cgaatctgtt ctcaacgaat cgcatgtttc gctgttcgca ctgcgcaacg
     5821 ggatgccgat acactgggcg tcatcttcgc acggtgcaga agttcaaaat gcgcaaagct
     5881 ggtcagcgac cattcgtcag cgtgatggtg cgcctgcggg gatcctgcaa attaaaacct
     5941 cgtcaggagc agaaaccagc gcctttatcg aacgcgtggc agatatcagc cagcatatgg
     6001 ccgcgctggc gctggaacag gaaaaaagcc gtcagcatat tgaacaactc atccaatttg
     6061 atccgatgac cggtctgcca aatcgcaata acctgcacaa ttacctcgat gaccttgtcg
     6121 acaaagccgt ctctccggtg gtgtatctca tcggtgttga ccatattcag gatgtgattg
     6181 atagccttgg ctatgcgtgg gccgatcagg cattgctgga agtggtcaat cgctttcgtg
     6241 aaaaactcaa accggatcag tatctctgtc gtatcgaagg tacgcagttt gtcctcgtga
     6301 gcctggaaaa cgacgtcagt aacattaccc aaatcgccga tgagctacgg aatgtggtca
     6361 gcaagccgat aatgattgac gataaaccat tcccgctaac cttgagtatt ggcatcagct
     6421 acgacgtggg taaaaaccgc gattacctgc tctccactgc tcacaatgca atggattata
     6481 ttcgcaagaa tggcggtaac ggctggcagt tcttcagccc ggcgatgaac gaaatggtaa
     6541 aagagcgttt ggttttaggc gcagcgctga aagaagcgat tagcaataac caactgaaac
     6601 tggtttacca gccgcaaatc ttcgcagaaa cgggtgaact gtacggcatc gaagcccttg
     6661 ctcgctggca cgatccccag catggtcatg tacccccttc acggtttatt cctctcgcag
     6721 aagagattgg tgaaatcgaa aatattgggc gctgggtcat cgcggaagct tgccgtcagt
     6781 tagcagaatg gcgtagccag aatattcata tcccggcgtt atccgtgaac ttgtcggcgc
     6841 tgcactttcg cagtaatcag ctgcctaatc aggtgtctga tgcaatgcaa gcctggggta
     6901 ttgacggcca ccagctgacg gtagaaatca cggaaagcat gatgatggaa cacgataccg
     6961 aaatctttaa gcgcattcag atcctgcgcg atatgggcgt gggcttatcg gtagatgatt
     7021 ttggcacggg cttttccgga ttatcccgct tagtcagtct tccggtaacg gaaatcaaaa
     7081 ttgacaaaag ttttgtcgat cgttgtctga cggaaaaacg catccttgcc ttacttgaag
     7141 ccattaccag cattgggcaa agcctcaatt taaccgtcgt ggcggaaggc gtcgaaacca
     7201 aagagcaatt tgagatgcta cgcaagatcc actgtcgcgt tattcaggga tatttctttt
     7261 cccgccccct acccgccgag gaaattccag gctggatgag cagcgtgtta ccgctgaaaa
     7321 tctgacaaat tcctctcgcc cgcactcgcg ggttttcatt taacgtgacg ctgtcactta
     7381 aatactgtga tttcagctac gattccggga gattccttcc ttaacttccc ctcttgcacc
     7441 aaaactgcac tacgctgcac aaatgttgat caaaagttaa aaaatataac tttaagccag
     7501 cattctggcg cttatcccgg catggcatga gatctgcata agcggaaagc gcagcaattt
     7561 ttgtcttaat acaggaaccg tttatgtcgg ataccaccga actggttgat ttagccgtga
     7621 tcttccctga tctggagatc gaattgaaat acgcctgcgc tgataacatc acaggtaaag
     7681 ctatttacca gcaagcgcgt tgtctgttac acaaggatgc gattaccgcg ctggcgaaaa
     7741 gtatcagtat cgcccagctg tcaggcttac aactggtgat ttacgatgcg tatcgcccac
     7801 aacaagcaca ggcgatgttg tggcaagcct gcccagaccc gcaatatgtt gttgatgtga
     7861 cggtcggttc taatcacagc cgtggcacgg cgatcgacct gacgcttcgt gatgagcacg
     7921 ggaacatcct ggatatgggc gcagggttcg atgaaatgca cgagcgctcc catgcctatc
     7981 acccttccgt cccgcccgcc gctcagcgca atcgcctgtt gctgaatgcg ataatgactg
     8041 gtggcggttt tgtcggtatc tccagcgaat ggtggcactt cgaattaccc caggcagcga
     8101 gttaccctct gctcgctgat caattctctt gttttatatc ccctggcaca cagcacgtca
     8161 gttaatcagg aaactgtcat gaagagatcg atatcgtttc gtcccacatt gctcgcgctc
     8221 gtccttgcca caactttccc ggttgcgcac gccgccgtac cgaaagatat gctggtgatt
     8281 ggtaaggccg ccgatccaca aaccctcgac ccggcggtaa caatagataa taacgactgg
     8341 acagtgacct acccgtctta tcagcggctg gttcagtaca aaacggacgg tgataaaggc
     8401 tcaaccgacg ttgaaggcga tctggcaagt agctggaaag cgtctgacga tcaaaaagag
     8461 tggacgttca ccctgaagga caatgctaaa tttgccgatg gcacacctgt cactgccgaa
     8521 gcagtaaaac tctcttttga gcggttacta aaaatcggcc aggggccagc agaagcattt
     8581 cccaaagatt taaagattga tactcccgac gaacatacgg tgaagtttac ccttagccaa
     8641 ccattcgcac cgttcctcta cacgctagcg aatgacggtg catccattat caatccggcg
     8701 gtcttaaagg aacatgcagc ggatgatgct cgcggcttcc tcgcgcaaaa taccgccggt
     8761 tccggaccat ttatgctgaa aagcgggcaa aaaggtcagc aattagttct ggtgccaaat
     8821 ccgcattacc ccggcaataa accgaacttt aagcgagtat cggtaaaaat tatcggtgaa
     8881 agtgcctccc gtcgcctgca gctctcccgt ggcgacattg acattgccga tgcgctgccg
     8941 gtggatcaac tcaacgccct gaagcaggaa aataaagtca atgtggcaga gtatccgtca
     9001 ctgcgcgtta cctatctgta tctgaataac agcaaagcgc ctcttaatca ggcggatctg
     9061 cgtcgggcca tttcctggtc taccgattat cagggcatgg ttaacggcat tctgagtggt
     9121 aacggaaaac agatgcgcgg cccgattccg gaaggcatgt ggggctacga tgcgacggca
     9181 atgcaataca accatgacga aacgaaagcc aaagccgaat gggataaagt gacgagcaaa
     9241 cccaccagcc tgacgtttct ctactccgat aacgatccga actgggagcc tattgctctg
     9301 gcgacacaat ccagtctcaa caagctgggc atcaatgtga agctggaaaa gctggcgaac
     9361 gccaccatgc gcgacagagt gggtaaaggt gattacgaca ttgcgattgg caactggagt
     9421 ccggattttg ccgacccgta tatgtttatg aattactggt ttgagtcaga caaaaaaggt
     9481 ctgccgggta accgctcgtt ctatgaaaac agtgaggtcg ataagttact gcgcaatgcg
     9541 cttgcgacca ccgaccagac gcagcgtacc cgggactacc agcaggcaca gaaaatcgtc
     9601 attgatgacg ctgcttatgt gtacctgttc cagaaaaact accaactggc gatgaacaaa
     9661 gaggtgaaag gctttgtgtt caatcccatg ctggaacagg tcttcaatat caataccatg
     9721 agtaaataac gtgaccttct ggagtatttt acgccaacgc tgctgggggc tggtgctcgt
     9781 ggtggcgggc gtctgcgtga ttacgtttat tatctcgcat ctgatccctg gcgatccggc
     9841 gcggttactg gcgggtgacc gcgccagcga tgctatcgtg gaaaatattc gccagcaact
     9901 gggactggac cagccactgt atgtacagtt ttaccgctac ttcagcgacc tgtttcatgg
     9961 tgacctggga acatccattc gtaccgggcg tccggtgctg gaagagttgc gtatattttt
    10021 cccggcgacg ctggaactgg ctttttgtgc cctgctgctg gcactcctga ttggcatccc
    10081 gctgggcata ctctctgcag tctggcgaaa tcgctggctg gatcatctgg tgcgaataat
    10141 ggccattacc ggaatctcca cacctgcgtt ctggcttgga ctgggcgtca ttgtgctgtt
    10201 ttatggtcat ctgcaaattc ttcccggcgg cggaaggctt gatgactggc tggatccacc
    10261 aacgcacgtt accggctttt atctgctcga tgcgctgctt gaaggcaacg gtgaagtctt
    10321 cttcaatgcg ttgcaacatc tcatcttacc ggcattaacg ctggcgttcg ttcacctggg
    10381 aattgtcgct cgccagatcc gctcagcgat gctggaacaa ttgagtgaag actacattcg
    10441 taccgcccgg gccagcggct tgcccggctg gtatatcgtt ttatgttatg cgctacccaa
    10501 tgcgttgatc ccatcgatta ccgtattggg tctggcgctg ggcgatttgt tgtatggcgc
    10561 agtgctcacc gaaaccgttt ttgcctggcc cggaatgggt gcatgggtag taacatcaat
    10621 acaggcgctc gacttcccgg cagtgatggg ctttgccgtc gtggtttcat ttgcttatgt
    10681 gctggtcaac ctggtggtgg atttgctcta tttgtggatt gatccgcgta tcggacgtgg
    10741 aggtggtgaa tgatgctaag cgaggaaacg tccgccgtac gcccacaaaa acaaacgcga
    10801 tttaacggtg caaaactggt atggatgctg aaaggcagtc cgctcaccgt gaccggcgca
    10861 gtcatcattg tattaatgct attgatgatg attttttcac cgtggcttgc gacgcatgat
    10921 cccaacgcca ttgatttaac cgcccgcctt ttgccgcctt ctgcggcgca ctggtttggc
    10981 accgatgaag tgggacgcga tctgttcagc cgcgtactgg tcggcagtca gcaatcaatt
    11041 ctcgccggat tagtcgtggt tgccattgcg ggtatgattg gttcgctact cggatgtcta
    11101 tccggtgtgc ttggcggacg cgcagacgcc atcatcatgc gcatcatgga cattatgctg
    11161 tcgattcctt cgttggtact gacaatggca ctggctgccg ctctcgggcc gagtttattt
    11221 aacgccatgc tggcgattgc tattgtgcga attccctttt atgtgcgcct ggcgcggggg
    11281 caagcattag ttgtacgcca gtatacctat gttcaggcgg cgaaaacctt tggtgcgtcg
    11341 cgttggcatc tgatcagctg gcatatttta cgtaactccc tgccgccgct gatcgtgcag
    11401 gcatcgctgg atatcggtag cgcgatttta atggccgcca cgttgggatt tattggcctg
    11461 ggtgctcaac aaccgagtgc tgaatggggc gcgatggtag ctaatggtcg caactatgta
    11521 ctcgatcagt ggtggtattg cgcatttccg ggggcagcga ttttgcttac cgccgtcggg
    11581 tttaatctct ttggtgatgg tattcgcgat ctgcttgacc cgaaagcagg aggaaagcag
    11641 tcatgaccca acccgttctg gacattcaac agctgcattt gagtttcccc ggttttaacg
    11701 gttatgttca cgtgctcaac aatgtgtcct tgcagattaa ccgcggtgaa attgtcggtc
    11761 tggtgggaga atccggctca ggtaaatcag tcaccgcaat gctgattatg cgtctgctac
    11821 cgacgggcag ttattgcgta catcgggggc agatttcact gctgggagac gatgttctta
    11881 acgctcggga gaaacagctt cgtcagtggc gcggcgcacg agtggcgatg atctttcagg
    11941 aaccgatgac cgccctcaat ccgacacgtc gaataggtct tcagatgatg gacgtgatcc
    12001 gccatcatca accaataagt cgtcgggaag ccagagctaa agcgattacc ctgctggaag
    12061 agatgcaaat cccggatgcc gtggaagtta tgtcgcgcta tccgtttgag ctttcaggtg
    12121 gtatgcgcca gcgggtaatg attgcgctgg cattctcctg cgagccgcaa ttgattattg
    12181 ccgacgaacc gactacggcg ctggacgtca cggtacagtt gcaggtactg cgtttgctta
    12241 aacataaagc tcgcgccagt ggaactgcgg tactgttcat cagccatgat atggccgtgg
    12301 tgtcgcaact gtgcgatagc gtttacgtga tgtatgccgg aagcgtaatt gaaagcggcg
    12361 tgacggcaga cgttatccat catccccggc atccgtatac cattggtttg ctgcaatgcg
    12421 caccagaaca tggagtacca cgccagccat tacccgccat tccagggacg gtaccaaacc
    12481 tcacccattt gcctgacggc tgcgcttttc gcgatcgttg ctatgcggca ggtgcacagt
    12541 gtgaaaacgt cccggcgctg acagcgtgtg gtgacaacaa ccagcgctgc gcctgttggt
    12601 atccgcagca ggaggtcata agtatctgac acgttattaa cgttacgcga cgtccatatc
    12661 aatttcccgg cccgtaaaag ctggcttggt aaaactacgg aacatgttca tgcgattaat
    12721 ggtattgatt tacagatccg ccgtggtgaa accttaggga tcgtcggtga gtcaggctgc
    12781 ggcaaaagca ccctcgcaca gcttttaatg ggtatgctgc aaccgagcca cgggcaatac
    12841 atccgttcag gatcacaacg cattatgcag atggtgtttc aggacccgct ctcttcgctt
    12901 aatccgcgct tatcggtgtg gcgcatcatc acagaaccgc tctggatagc taagcgtagt
    12961 agtgaacaac agcggcgagc gttggcaaag gagttggctg tgcaggtggg tattcgtccg
    13021 gagtatctcg accgcctgcc tcatgcgttc tccggcgggc agcggcaacg catcgccatt
    13081 gccagagcac tctcttcgca gcctgacgtg attgtgcttg atgagccaac ctcggcgctg
    13141 gatatctccg tgcaggcgca gatcctcaat ttactggtaa cgctacagga aaatcacggg
    13201 ctgacttatg tgctgatttc acacaatgtc tcggtgatac gtcatatgag cgatcgggtg
    13261 gcggtgatgt atctcgggca gattgtagaa ctgggcgacg cgcagcaggt gctgacggca
    13321 cctgcacatc catacacccg attattgctg gattccctcc ccgccattga taaaccgctg
    13381 gaggaagaat gggcattatg taaaacggat ctgccgggaa accgcacgtt gccacaaggc
    13441 tgttttttct acgaacgttg ccctctggca acccacggat gtgaagtccg gcaatcatta
    13501 gcaataaggg aggacggacg tgagctccgg tgctggcggg cgctgtagag atgctaacaa
    13561 cgcatcaggc attcacgcgc ctgacgcgtc atccggcaat gctttacgat ttcaactggt
    13621 aatccagcgt aatttccgct ttcagtacct gagaaaccgg gcatcctgct tttgctttct
    13681 ggatgatgcc gtcaaaggta gaggcatcaa tacccggcac cgcaacttca ctcttcagtg
    13741 cgattttcgt aatcgcaaaa tcggcatcca ctttatccag cgacacatcg gcggtggtat
    13801 caatcgatgt tggcgtgaat cccgcttccc ccagcattaa tgaaagcgcc attgagaaac
    13861 atgcggcatg cgctgcgcca atcagttctt cagggttggt tcctttttcg ccttcaaaac
    13921 gcgtgttaaa tccatacggc tgttggttca gcacgccact ctgggtggat actgttccct
    13981 tcccgcgttt gatatcgcct tcccagtgtg cctgaccttt cttatggatt gtcattgttg
    14041 ctctcctgtg ggcttaaaaa ggaaattata gactacgtga gaaacagcac gaggataagc
    14101 aggatattcc gaataaaata cggcgaaaat attacttctg cacttaaatt aaattcccac
    14161 aatagataac cgggaatata tatctcaaga gatattgcat caatatcaat caactcacca
    14221 caataataat acatatcaat atcatggagt taaaatataa aaatcatcaa ccaggactaa
    14281 tcttaacaac gaagcggcaa atatttgcca tgctgaatgt gctttataaa tctgcgatcc
    14341 gtagcagaca ccataaatac acagacacgg agaatcacta tgtttactta ttatcaggca
    14401 gaaa
//
LOCUS       DAJVRF010000102        14391 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJVRF010000102 DAJVRF010000000
VERSION     DAJVRF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14391
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.102"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            255..2021
                     /gene="adeD"
                     /locus_tag="OQN13_002833"
     CDS             255..2021
                     /gene="adeD"
                     /locus_tag="OQN13_002833"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCS1616137.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDVEKFAFTTLEVTE"
     gene            complement(2066..3457)
                     /gene="uhpT"
                     /locus_tag="OQN13_002834"
     CDS             complement(2066..3457)
                     /gene="uhpT"
                     /locus_tag="OQN13_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCS1616138.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3595..4914)
                     /gene="uhpC"
                     /locus_tag="OQN13_002835"
     CDS             complement(3595..4914)
                     /gene="uhpC"
                     /locus_tag="OQN13_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCS1616139.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4924..6426)
                     /gene="uhpB"
                     /locus_tag="OQN13_002836"
     CDS             complement(4924..6426)
                     /gene="uhpB"
                     /locus_tag="OQN13_002836"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCS1616140.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6426..7016)
                     /gene="uhpA"
                     /locus_tag="OQN13_002837"
     CDS             complement(6426..7016)
                     /gene="uhpA"
                     /locus_tag="OQN13_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCS1616141.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(7091..7381)
                     /gene="ilvN"
                     /locus_tag="OQN13_002838"
     CDS             complement(7091..7381)
                     /gene="ilvN"
                     /locus_tag="OQN13_002838"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS1616142.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7385..9073)
                     /gene="ilvB"
                     /locus_tag="OQN13_002839"
     CDS             complement(7385..9073)
                     /gene="ilvB"
                     /locus_tag="OQN13_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCS1616143.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(9179..9277)
                     /gene="ivbL"
                     /locus_tag="OQN13_002840"
     CDS             complement(9179..9277)
                     /gene="ivbL"
                     /locus_tag="OQN13_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCS1616144.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9842..9931
                     /gene="tisB"
                     /locus_tag="OQN13_002841"
     CDS             9842..9931
                     /gene="tisB"
                     /locus_tag="OQN13_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCS1616145.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            10211..11395
                     /gene="emrD"
                     /locus_tag="OQN13_002842"
     CDS             10211..11395
                     /gene="emrD"
                     /locus_tag="OQN13_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCS1616146.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11403..11900)
                     /locus_tag="OQN13_002843"
     CDS             complement(11403..11900)
                     /locus_tag="OQN13_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCS1616147.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11897..12259)
                     /locus_tag="OQN13_002844"
     CDS             complement(11897..12259)
                     /locus_tag="OQN13_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCS1616148.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12249..12596)
                     /locus_tag="OQN13_002845"
     CDS             complement(12249..12596)
                     /locus_tag="OQN13_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCS1616149.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12705..13154
                     /locus_tag="OQN13_002846"
     CDS             12705..13154
                     /locus_tag="OQN13_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616150.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13403..>14391)
                     /locus_tag="OQN13_002847"
     CDS             complement(13403..>14391)
                     /locus_tag="OQN13_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1616151.1"
                     /translation="PSQPLQQSLKDLERAYKNFFQNRAAFPRFKKRGQNDVFRYPQGV
                     KLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPVHPS
                     ASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRK
                     IQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSLPGRNVRAK
                     SGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPAYTSQRCVCCGHTAKENRLSQSK
                     FRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3396 a         3972 c         3732 g         3291 t
ORIGIN      
        1 gtcccatgct gaggtagttt aaataatcgc gaaagcgtcc ctgattcatt actcacgtaa
       61 tcggtattgt cattattcat ttttttgtcc attggaggag atttaatccc ttgcttttgt
      121 aaaacaaatg acaacaacgg ttcagtgata attatcacat ttcaattgca cattaatgga
      181 tattctttaa taatctcgcg acgtttcttt atgataaata ataatcaaat tgataaaatc
      241 aaaatgagaa aaatatgaat aattctatta accataaatt tcatcacatt agccgggctg
      301 aataccagga attgttagcc gtttcccgtg gcgacgctgt tgccgattat attattgata
      361 atgtctctat tctcgacctg atcaatggcg gagaaatttc cggcccaatt gtgattaaag
      421 gacgttacat tgccggtgtt ggcgcagaat acgctgatgc tccggctttg cagcggattg
      481 atgctcgcgg cgcaacggcg gtgccagggt ttattgatgc tcacctgcat attgaatcca
      541 gcatgatgac gccggtcact tttgaaaccg ctaccctgcc gcgcggcctg acgaccgtta
      601 tttgcgaccc tcatgaaatc gtcaacgtga tgggagaagc cggattcgcc tggtttgccc
      661 gctgtgccga acaggcaagg caaaaccagt acttacaggt cagctcttgc gtacccgccc
      721 tggaaggctg cgatgttaac ggtgccagtt ttacccttga acagatgctc gcctggcggg
      781 accatccgca ggttaccggc cttgcagaaa tgatggacta ccctggcgta attagcgggc
      841 agaatgcgct gctcgataaa ctggatgcat ttcgccacct gacgctggac ggtcactgcc
      901 cgggtttggg gggtaaagaa cttaacgcct atattactgc gggtattgaa aactgccacg
      961 aaagttatca gctggaagaa ggacgccgga aattacaact cggcatgtcg ttgatgatcc
     1021 gcgaagggtc cgctgcccgc aatctcaacg cgctggcacc gttgatcaac gaatttaaca
     1081 gcccgcaatg catgctctgt accgatgacc gtaacccgtg ggagatcgcc catgaaggac
     1141 acatcgatgc cttaattcgc cgcctgatcg aacaacacaa tgtgccgctg catgtggcat
     1201 atcgcgtcgc cagctggtcg acggcgcgcc actttggtct gaatcacctc ggcttactgg
     1261 cacccggcaa gcaggccgat atcgtcctgt tgagcgatgc gcgtaaggtc acggtgcagc
     1321 aggtactggt gaaaggcgag ccgattgatg cgcaaacctt acaggcggaa gagtcggcga
     1381 gactggcaca atccgctccg ccatatggca acaccattgc ccgccagcca gtttccgcca
     1441 gcgactttgc cctgcaattt acgcccggaa aacgctatcg ggtcattgac gtcatccata
     1501 acgaattgat tacgcactcc cactccagcg tctacagcga aaatggtttt gatcgcgatg
     1561 atgtgtgctt tattgccgta cttgagcgtt acgggcaacg gctggctccg gcttgtggtt
     1621 tgcttggcgg ctttggactg aatgaaggtg cgctggctgc gacggtcagc catgacagcc
     1681 ataatattgt ggtgatcggt cgcagtgccg aagagatggc gctggcggtc aatcaggtga
     1741 ttcaggatgg cggcgggctg tgcgtggtac gtaacggcca ggtccaaagt catctgccgt
     1801 tgcccattgc cggcctgatg agcaccgaca cggcgcagtc gctggcggaa caaattgacg
     1861 ccttgaaagc cgccgcccgt gaatgcggtc cgttacccga tgagccgttt attcagatgg
     1921 cgtttctttc tctgccagtg atccccgcgc taaaactaac cagtcagggg ctatttgatg
     1981 tcgagaagtt tgccttcact acgctggaag tcacggaata ataaaaaagc ccggcgtcat
     2041 gccgggcaaa agtcaccagt tacgtttatg ccactgtcaa ctgctgaatt tttttctcgc
     2101 ggcggatttt gcgttcttcc atcaccgcca ctatcgccat caggcagata caaccaatcg
     2161 cggcgatatc aagtgcggcg aaggtgcctg cccagccggt aaggccgaat accggcgtcc
     2221 catcggcaat cattcccaga cctaacttgg caaagctgtc accaatcagg taagcaaagg
     2281 tgcctttaat accatcggca gcgccaatcg cttttttagg tacaaagcca acagcagcca
     2341 caccaatcaa caattgcggg ccaaagacca ggaaacccaa cgcaaagaga gaagccagat
     2401 agatatattc gttactggca tgttgataca caccgagcgt ggcgataatc agcgccagcg
     2461 cgatgcaggc caccaggcca cggcgaccgt tcgccaggtc agagagccag ccccacagca
     2521 gcgtaccgac cagcgcacca gcttcaaaca gcgtaaagcc ctgaatcgcc accgctttag
     2581 agagtttcag ttcctggaac gcgtatacgg ttgaccactg gtcgatacca atacgtacca
     2641 catagaggaa aatgttggcg aagcacagca gccagatcac tttgtttttc agcacatact
     2701 caacaaagat ctgccacttg gtcatatcgg tagattctgt ctctttgtcc tcttcgctga
     2761 tctcctcgcc gaacagttct tcagctttgc cgaggccata agattccggg gagtcgctgc
     2821 cgtaacgcag gccgataaaa ccgacaatca gcgcgataat cgacgggaag ataaacatgc
     2881 cgatgacatg gccatcgaac aggtaatttg ccccgaacag cgccacgcct gctgcgcctg
     2941 caccgccaag gttgtgagaa atattccaga aaccgaggaa tgtcccgcgt ttacgacgcg
     3001 gcgtccattt ggtgatggtg gagtaactgc acgaaccgcc ggtactctgg aaaaagccgc
     3061 ttaaggcgta gaaggcaatc atcaggaaca ggctaaccga gccgctgccc atactggcac
     3121 tgaagcccag catacaaatg gcagacagga tcagcatgaa cggcaggaat tgtttggtgt
     3181 ttttgccgtc ggcgtagtag gaaaccagcg ttttacccac gccataagtg atggagaaac
     3241 ccaggccgat catccccagc tgcgtcatgc tcaacccgta ggtcgagatc atgtcgttct
     3301 gcgcgatgtt aaagttcttg cgaatcaggt acatcgtcag gtagccgata aagaccacca
     3361 ggtaggattg catgaacggt ttgaaccaca ttttgcgccg cacttcgagc ggaaggtcca
     3421 gggtcggctt gcgaacctgg tttaagaaag ccagcatggg ttactcctga aatgaatacc
     3481 tgcgtgaggc atgcattgta aaaatcagca gcaaatcagg cctgagatag cgtccaggca
     3541 aaacctggaa aatttcttag ttttgccgga tatgaagtga aaaggtgaga tgcatcacgc
     3601 ttcgcgcggt gtctgggcgt tcaaaaaggg cagtaacagc agtgcggaaa tcccggcggc
     3661 gatagagata accacaaaaa atccgctcca gtgccaggta tcgagtactt tcgccagcgg
     3721 ccaaccagca agcgacgccc ccagataagc aaacaagccg acaaaccccg tcgccgcccc
     3781 tgccgcctct ttgtgggaac actctgccgc cgccataccg attaacatct gtgggccaaa
     3841 gacaaaaaaa ccaatggtga agaagcaggt tgcctgcatc acgtagctgg caaatggcat
     3901 cagccacagg gagccgactg aaagcaaaat tccggcggcg aaaatcaaat tcatcggccc
     3961 tcggttgccg ttaaacaatt tgtccgagcc ccaacccgct accagcgcac cgataaatcc
     4021 acccagttca aacatcgtca ctgccgtatt cgccgtgacc agatcgacgc ccagcgtctc
     4081 ggacatatac aaattgcccc agtcgttgat cgccgcccgg accacataga ccagcacata
     4141 gcaaaacgaa agcagccaga tatacggatt cagcaacaca tatttggtga ggatctcttt
     4201 acgcgtcagc cctgcccctt cttgttgttg agctatctcc agcgcgtcgt gtcgccattc
     4261 accgaccgcc ggtaaaccta acgcctgcgg gcgatcgcgc agccgccagc agagaaaaat
     4321 tcccacgacg atcgccatac aaccagcaat catcatcccg gcacgccagc cgtaatgtag
     4381 tgcagccgct gccatcacaa tgggaatgag tgcgccgccg acgttatgcg ccgtgttcca
     4441 taatgcccac caaccaccgc gctcggtacg tgaataccag gccgttaaca gacgcgcaca
     4501 caccggtgaa ccccagccct ggaaaaaggc gttcagcacc cagagtacgg caaacgccca
     4561 tagcgacgtc gagaagccaa acagaatgtt gataatgccc gtggcgataa gccctatccc
     4621 cataaaataa cgggcatttg agcgatcgct gacaatgccg gagacaaact tcgacacgcc
     4681 ataggtaatg taaaacaggg tcgctaacag gccgatatcg ctacggctga gcacgccgtt
     4741 agcaaggatt tctggtacgg cggcgttaaa acttttccgc gtgaagtaaa acagcgcgta
     4801 acccagccag atggtcagca gaatatgccg acgccagtag cgatagcggg catcaatttc
     4861 atatttatca gccattaatg gcgcatcggc aggcgctttc agaaacggca acatcatcaa
     4921 accttagaca tagcgttgag gtagagaaac gctgacacgc gtgccgtgca gacaggaaat
     4981 gtgtaatgtg ccacccagcg ccgttacgcg ctcgcgcatt ccggtgaggc caaaaccttg
     5041 ttgcccggaa cccggcggca aaccgctgcc atcgtcttca ataaccagca tcaaccgttc
     5101 atcctgctgc cagccttgca gggtgaccgc gctggcatca gcatgtttca caatgttgtt
     5161 cagcccttcc tggcagacac gaaacagcgt cacgcgctgg ttttcgctta acgctgattc
     5221 atcgattcgc cattcgagat ggctgacaat accgcgccct tccagctcca tttcccgcat
     5281 cagtgagcgg atggcctgct ccagggtgag atcatccaac tggcgcggac gtaaccgacc
     5341 caacaaacgg cgcaccgcgt cgtaaacgcc cagcgatagt tgttcgatga gttgcccgct
     5401 ctgcttcacg ctggcgttat ctgccgccag ccgctgaaca atgcccgcct gagtacgaat
     5461 agcagtgatg gtctggccga tatcatcatg cagctcacgc gccacatcac ggcgcacgct
     5521 ctcttcggtt tccagcaacc gttcagccag atgctgattg cgcgccagtt ccttttgcag
     5581 cgactggtta agttcacgca accgctggat gccagcgcca agcaacaacc ctgtcagact
     5641 ttgcaccagc agcgagagca ataaatccac cggatgatcg cgccaggttt gactggcgat
     5701 cagcgcgatg gcgttcatca acgtcgcaat cagcgcccct tgccaaccat agtgccaggc
     5761 cagcgcgata atcggcagca ccagacagaa tggcgtaaag cgcgacagtt cgtccggcaa
     5821 tcccaactgg agccagagac tgataacaaa cagcagcaag taccagacca gatgtcgccc
     5881 gcgccagttg attggctgag aaaccagtga cgggccgagc ggcagccagg tgttattggc
     5941 gagatagtgc cagaacacca gacatatcgg ggccagcgtc aggccgccag ttaaagtcag
     6001 caacagcgca ttccacgact ctttgccgtg ccaaagccag ggcagcgact gcaacaacgc
     6061 cgccgccgtt aacgccgccc cctgtaacag caaggtgcgc cagtcacgct gatggcgata
     6121 gcgcgagatc agcgctacgg gcagtaacgt cagtaaacta ccgatcatca ataacggaaa
     6181 atgggttaaa ccgaccgcct gcgttagcca gtaaatcagc agccactccg cacccagcaa
     6241 tacgggccag tatccgcgcg ggcattgcag cattagcccc agacgcagac caaacggaaa
     6301 taacagcacc gccatatcag ggcgctcaac cagatgcagg ctgatactcc acaggcaaaa
     6361 ccatgcggca gagaagataa aaaagcaggc aataacggta attaagcggg agaacaacgt
     6421 cttcatcacc agccatcaaa catgcggcgc gccagctcta cgtcgttact gacgcccagt
     6481 ttttccatca gattggcgcg atggacgtgt accgttttcg gtgacaagcc cagttcggcg
     6541 gcaatctctt tcaccgccat tccttgcgcc agtttttccg ccacctggcg ttcacgtttg
     6601 gttagcgggt cctgacgacc ggatgccagt ttaatggcaa tatccggcgt cagataacag
     6661 ccacccgtgg caaccgtatg caccgcagca atgagttcat ccgggctaca gcgtttggaa
     6721 agaaagccgc gtgcccccgc gttaagcgcc tgctcaacca gcgcaggact gtcgtgaacg
     6781 gagagcataa tcgtcgccat acctttcggc agctggctta gcagctccag accggagata
     6841 tcgggcatgg agatatcgca aatacacacc tgcacaccgc gccccggcag ccccgccagc
     6901 gcctcgcgcc ccgaaccaaa ctcggcaact acctgcaaat caggttccag ccccagcagc
     6961 tgcgcaaagc cggagcggac gatgaggtga tcgtctataa gggcaacggt gatcatggtc
     7021 ttgtcctggc gggtaaaaaa aaacgcgctt accttaacga taagcgcgat gttgttcaag
     7081 ccttgagcgg ttactgaaaa aacaccgcga tcttgttaaa catcgtcgga tcggactgat
     7141 tacgctgcac tttcacgaca tcttccagct tatcgatttg gcttatcatc tgctccagac
     7201 gctggtcgtc attgaccagt agccagatat ggcttttgtc gctgtcctga atcggcagac
     7261 aaagaatgcc ttcaacgtta aaagcgcggc gggcaaaaag gccacaaacg tgggtcatta
     7321 cgcccggatg gttgcgaacg gtgagctcca gaattacgtt gtcatgagtt gtgttttgca
     7381 tggcttattc ccccaccatt tcagtattcg ccgcacctgg cggcaccatc gggtaaactt
     7441 tttcttcggc atcaatgcgc acatggatca gcgccgggct agggcgattg atgatttcct
     7501 gcaatgcagc ctgcggatcg gcttcgttat tcaaatcaca ggtttcgagg ccgaatccgg
     7561 cggcaatctg cataaagttg attttgcccg gataggtggc ggcaaaaacg ccttgctcgt
     7621 agaacagact ctgttgctga tgcaccagcc ccagcgcttc gttgttcatc agaatgattt
     7681 tgacatccag ctgattttca ctggcggtcg ccatctcctg aatattcatc atcaggctgc
     7741 cgtcgccgga gaaacacaac actttgcgat ccgggttcgc cagcgccgcg ccaatcgccg
     7801 caggcaggcc aaaacccatc gtgcccagcc caccggaggt cagccactgg cgtgggcgat
     7861 tgagcggata agcttgcgcg gtccacatct gatgctgacc cacatcggtg gtgataatcg
     7921 cattgtcatc gacacaggcg gcaacggcgt tgatcaggcc gtaatggctt aatggatcgc
     7981 acgctttcgg gattggacac gggaattcgc gctgcaaatc cgctaccaac tggtgccact
     8041 ctgcacgcgg ttgcgcttcc acctgcggga tcaattgcgc cagcacgtca tcaacatccg
     8101 cctgaatcgc cacatgcggc tgcttgattt tacccagctc tgcgcgatcg atatcgacat
     8161 gaataatttt ggcattcgga cagaactgct cggttttgcc aatcgcccgg tcatcaaaac
     8221 gcgcaccgag cacaatcaac aaatccgcct cctgcaaaat atagttggta ctgcgcacgc
     8281 cgtgcatccc cagcataccc agcgacaacg gatgcgcttt tggcagcatg cccagcgcca
     8341 ttaaagtcat ggtggtaggc agttgcgctt tctccgccag ttcacgcacc cgcgcaggcg
     8401 cattgatcac accaccaccc agataaagca ccgggcgttt ggcagcgtta atcattgtag
     8461 ctacgtcacg aatgctttct tcgctaaagg cgggtgcagc ggctttttct gccaccgcgg
     8521 gctgagcttc aatctcaaaa accgccgttt gtacatcctt aggaatgtct atccacaccg
     8581 ggccagggcg gcctgattgc gcaatgcgga aggcatcgct catgacctgc gggagttctt
     8641 cgatatgtct gaccagatag ttgtgtttgg tgatggggat agagatgccg taggtgtcga
     8701 cttcctggaa ggcgtcggtg ccgatcatcg aggcgggaac ctgaccagtg atgcaaatca
     8761 gcgggatgga gtccagccgc gcatcggcaa tggcggtcac caggttagtc gcacccggtc
     8821 cgctacaggc catacagacc gccggtttac cgtcggtgcg cgccattccc tgagcgataa
     8881 agcccgcgcc ctgttcatga cgggccagaa tatggcggat ttgcgtgctt tggcttaagg
     8941 catcgtaaac aggcaggata gaaccgcccg gaatgcctgt cacaatctta atgccctgct
     9001 gttccaggaa atgaacgata aattctgcgc cggtaaagcg cttacgcgtc aatgttgtgc
     9061 ccgaacttgc catgctccag tccttttctt ctgggccgac tttccgggag gtgcttaaac
     9121 gaaaaacccc gcccggtttg cgccgacggg gttttggaat cgtgtgttgt tccagtccct
     9181 acggcgcatt gccgacgacc accaccacac gcacgacgac cactgcggcg gatggcgcag
     9241 ttggtagtag ttttgcgttg aacatggaag tagtcattgg ggaccttgtt ggttttgtgt
     9301 ttaacaatat ttatacaagc acagctttac aggggagaca atggaaaatt tttcagcaag
     9361 ggaaaattga ggggttgatc acgttttgta ctgaattgca gataacaaaa aaccccgccg
     9421 gagcgaggtt tcgtcagtcg cccgcggctg gtaaccgcaa agcacactgt attatgtcaa
     9481 cacagaaagt atacgtgttc cgcgcagaac gcgcaatttc ggcacgaatt ttgacgtatt
     9541 tagtgcatag ttgagtatcg atcacagttt gcgttttgtc caaatattgc tgtttattta
     9601 tacagtaaac ttctataata tcactgtacg caatgtgtta tgcgggggcc gcatcgttac
     9661 ccggcgcact aagtcctggc tgaaacgggt ggtgccgtca gcgccttaac cccgcgtgag
     9721 cacactgtgt tatgtcaaca agcacaaagt ttctccttga gataccgcgt gcacaacagc
     9781 tggcaacagg cagcggaaag gtacgtcagc tggcagtgct cctgaaccac aggagacgcg
     9841 tatgagcctg gtggatatcg ccattcttat cctcaaactc attgttgcag cactgcaact
     9901 gcttgatgct gttctgaaat acctgaagta attcagattc aagtcgcacc aaaggggagc
     9961 gggtaaccgc tcccctttta tatttagcgt gcgggttggt gtcggatgcg atgctgacgt
    10021 atcttatccg ccctaccatc tctcccggca acatttattg ccgcttttgt ttacatattc
    10081 tgccgctaaa caactcccca ttcctggcgt atatctggct aacattcatc aatgtgatag
    10141 attcctctcc cgcatttatg ggaatgcgta gtgacttact ctaattattt ttataaaagc
    10201 atccgtgata atgaaaaggc atagaaacgt caatttgtta ttgatgttgg tattactcgt
    10261 ggccgtcggt cagatggcgc aaaccattta tattccagct attgccgata tggcgcgcga
    10321 tctcaacgtc cgtgaagggg cggtgcagag cgtaatgggc gcttatctgc tgacttacgg
    10381 tgtctcacag ctgttttatg gcccgatttc cgaccgtgtg ggtcgccgac cggtgatcct
    10441 cgtcggaatg tccattttta tgctggcaac gctggtcgcg gtcacgacct ccagtttgac
    10501 ggtattgatt gccgccagcg cgatgcaggg gatgggcacc ggcgttggcg gcgtaatggc
    10561 gcgtactttg ccgcgagatt tatatgaacg gacacagttg cgccatgcta acagcctgtt
    10621 aaacatgggg attctcgtca gtccgttgct cgcaccgcta atcggcggtc tgctggatac
    10681 gatgtggaac tggcgcgcct gttatctctt tttgttggtt ctttgtgctg gtgtgacctt
    10741 cagtatggcc cgctggatgc cggaaacgcg tccggtcgat gcaccgcgca cgcgcctgct
    10801 taccagttat aaaacgcttt tcggtaacag cggttttaac tgttatttgc tgatgctgat
    10861 tggcggtctg gccgggattg ccgcctttga agcctgctcc ggcgtgctga tgggcgcggt
    10921 gttagggctg agcagtatga cggtcagtat tttgtttatt ctgccgattc cagcagcgtt
    10981 ttttggcgca tggtttgccg gacgtcccaa taaacgcttc tccacgttaa tgtggcagtc
    11041 ggttatctgc tgcctgctgg ctggcttgct gatgtggatc cccgactggt ttggcgtgat
    11101 gaatgtctgg acgctgctcg ttcccgccgc gctgttcttt ttcggtgccg ggatgctgtt
    11161 tcctctggcg accagcggcg cgatggagcc gttccccttc ctggcgggca cggctggcgc
    11221 gctggtcggc ggtctgcaaa acattggttc cggcgtgctg gcgtcgctct ctgcgatgtt
    11281 gccgcaaacc ggtcagggca gcctagggtt gttgatgacc ttaatgggat tgttgatcgt
    11341 gctgtgctgg ctaccgctgg cgacgcggat gtcgcatcag gggcagcccg tttaagcgca
    11401 cgtcaccgca gcatcgtcat cagctccatg ggagaacgat gctgctttat cagatcacgc
    11461 atcacccgca tatgcggtgc ggagtaagaa taaaacgcct gatagcccgc acaaagcacg
    11521 ctgtctggcg tctcctgccg agcgtgcggg cacaggcgat gccagtcgca cgcctggcag
    11581 tcacagcgcg gttgcgggtt ttcgctgcca caccattttt gtaaggtggc gtcaaaaagc
    11641 tcaatcgaga tacgactgat atctgcccgc acccaacact cgaacagggc aattaaaaat
    11701 ttcccccaca gctctgctgt caccgaagcg gccgtcagat cgccccggtc atcaaattca
    11761 agatagggaa tgagctgtac ggagcggatg ccccgctgtt tgaaattgtg ataaaaatcg
    11821 gcaggttgca gagcatcctt gtactctaca accagtttat gcctgtcctc ttcagcatca
    11881 atgacctgac ttcctgtcat gcgactctct caataaatac gataagttga tgtatatgcg
    11941 ttacagcaaa cagtattgcg agagataaca ccgcgaggga gatcaaaaat ttgtcacgga
    12001 ctacgtgaaa ttgggaaaaa tcgctattcg tgacatccat taaattgcga ttacgggtgt
    12061 agtgccagag gatcagcgcc acgatggcga gaatgccaat cgaaatccag aataacatgc
    12121 ccgcctggtg ccagttgtgt ttgatagcca acgccatcaa cgcgccgtag cccagcatgg
    12181 tacgaaacca cgccagtgat gtgcgctccg gctgcagtcc tgggtcggca atgcgtctgg
    12241 ctttgcggct atccggcata caacaccagt cccataacga tcaccgccac gaccatcaat
    12301 attaagctaa tgattaataa gctgttggtg tagggcaggt cctctttcag acgcatcgct
    12361 ttttcattgc gtaaccagcg caggtagcca tacattgcta acccgccgga aaacagacac
    12421 aacaacaacg ccagcagttc acgaatgacg ggcgtggcga aatctggcgc aagttgatca
    12481 agcccaaccc ccgccgccag aaatcctagt gcggtacgga tccacgccag aaaagtacgc
    12541 tcattagcca gcgagaagcg gtaatcgggc gcttctccaa ggcgggaaat tttcattgtt
    12601 gctcctttgc aatttcacta actctctacc tttagcggtt ttttttaaga atttcgtccg
    12661 cccgtttcct gatatgcttt tgccatccgc tacaaggagg tcagatgggt atcatagcgc
    12721 aaaataaaat cagttcattg ggcatgctgt tcggtgccat cgctttaatg atggggatta
    12781 tccacttttc attcggccct ttttccgcac caccgccaac gcttgaaagc atcgtggcgg
    12841 acaaaacggc tgaaattaaa agaggtcttc tcgccggaat aaaaggcgaa aaaattacca
    12901 ccgtggagaa aaaagaggac gtagatgtcg ataaaatcct cgatcaaagc ggtatcgcac
    12961 tggccattgc tgcgctactt tgcgcatttg ttggcggtat gcgtaaggaa aatcgctggg
    13021 gcattcgcgg cgcgctggtt tttggcggcg gaacgctggc tttccatacc ttgctttttg
    13081 gtatcggcat cgtgtgcagc attttgctga tattccttat cttttccttc ctgaccggcg
    13141 gttcgctggt ttaagcagcc tgaataatgc ccgccgggtg atcatcagcg ggcattatcg
    13201 tcagaacttc tgttttttct cttccacctt caccccttgt gtcggtaggc cggtgtcata
    13261 gtcacgtacc accggcgaat agccatcttg tgggcgtgga cgaaatgccc ccatccagcg
    13321 cggccgtgca tctttacgcc acggacgcag gctccattgt tgacatcctc cacgccctga
    13381 aggacgtgga cccctgctac gttcaggctg tcgcctgaat catttcggtg ggttcctgct
    13441 tcaacgagcg gcctgactgc accatctctc cacaggcaag aacggcgtgc cccgccgcta
    13501 aaatgttacg agcgccattt acatcggcgt tcgctgtata tccacatacc tggcatctga
    13561 atttactttg tgacaggcga ttttctttcg ctgtatgacc acagcacacg caacgctggc
    13621 ttgtgtacgc tggcggaaca gcaagcacct gaccaccacg ccagagctgc ttgtacgcaa
    13681 gctggcggcg catttcatac cagccctgat ccagtatcga acggtttaaa cctgattttg
    13741 cccggacatt gcgccccggc aggctgaccg tacccgctgc tgactttgac atgttgctga
    13801 ccttcaaatc ctcaatgact atcattgcgt ggtttttgct gacggtcgtt gtgactttgt
    13861 gaaggtagtc cctgcggata tttgcgatac aggaatgcag tcgctgtatt ttgcgtttct
    13921 gcttctgcca gttgttgctg aatttgacct tgcggcttaa ctgtcgctga agtctcgcca
    13981 gcttcttctg gtttttctga aaactgttta caggctcaaa gactgtgcca tctgacagcg
    14041 tggcgagttt agccacgcca gcatccagcc cgaccattga tgctgaaggg tgaaccggag
    14101 ttgatacttc actttctgtc tgaatactga tgtaccactt accgcaggac tggctgacag
    14161 tgacattttt cacaacaccc gtgacctgac ggctgttccg gtagcgcatc cagcccagtt
    14221 tcggcagaaa aatacggctg ttttcctgat cgagcttaac gccctgcggg tagcggaata
    14281 catcattctg tccccgcttt ttgaatcggg gaaaagcagc ccgattctgg aagaagtttt
    14341 tgtaagcccg ctcaaggtct ttcagtgact gttgcaatgg ctgtgacggg g
//
LOCUS       DAJVRF010000103        14369 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJVRF010000103 DAJVRF010000000
VERSION     DAJVRF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14369
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.103"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..696)
                     /locus_tag="OQN13_002848"
     CDS             complement(<1..696)
                     /locus_tag="OQN13_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1616152.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            952..1107
                     /gene="rseD"
                     /locus_tag="OQN13_002849"
     CDS             952..1107
                     /gene="rseD"
                     /locus_tag="OQN13_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCS1616153.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1104..1679
                     /gene="rpoE"
                     /locus_tag="OQN13_002850"
     CDS             1104..1679
                     /gene="rpoE"
                     /locus_tag="OQN13_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCS1616154.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1712..2362
                     /gene="rseA"
                     /locus_tag="OQN13_002851"
     CDS             1712..2362
                     /gene="rseA"
                     /locus_tag="OQN13_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCS1616155.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2362..3318
                     /gene="rseB"
                     /locus_tag="OQN13_002852"
     CDS             2362..3318
                     /gene="rseB"
                     /locus_tag="OQN13_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCS1616156.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3315..3794
                     /gene="rseC"
                     /locus_tag="OQN13_002853"
     CDS             3315..3794
                     /gene="rseC"
                     /locus_tag="OQN13_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCS1616157.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3992..5791
                     /gene="lepA"
                     /locus_tag="OQN13_002854"
     CDS             3992..5791
                     /gene="lepA"
                     /locus_tag="OQN13_002854"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCS1616158.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGSLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5807..6781
                     /gene="lepB"
                     /locus_tag="OQN13_002855"
     CDS             5807..6781
                     /gene="lepB"
                     /locus_tag="OQN13_002855"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCS1616159.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKLGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7054..7734
                     /gene="rnc"
                     /locus_tag="OQN13_002856"
     CDS             7054..7734
                     /gene="rnc"
                     /locus_tag="OQN13_002856"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCS1616160.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7731..8636
                     /gene="era"
                     /locus_tag="OQN13_002857"
     CDS             7731..8636
                     /gene="era"
                     /locus_tag="OQN13_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCS1616161.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8648..9376
                     /gene="recO"
                     /locus_tag="OQN13_002858"
     CDS             8648..9376
                     /gene="recO"
                     /locus_tag="OQN13_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCS1616162.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9388..10119
                     /gene="pdxJ"
                     /locus_tag="OQN13_002859"
     CDS             9388..10119
                     /gene="pdxJ"
                     /locus_tag="OQN13_002859"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCS1616163.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10119..10499
                     /locus_tag="OQN13_002860"
     CDS             10119..10499
                     /locus_tag="OQN13_002860"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCS1616164.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10721..10801
                     /gene="shoB"
                     /locus_tag="OQN13_002861"
     CDS             10721..10801
                     /gene="shoB"
                     /locus_tag="OQN13_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCS1616165.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="OQN13_002862"
     CDS             complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="OQN13_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCS1616166.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            11470..12660
                     /locus_tag="OQN13_002863"
                     /pseudo
     CDS             11470..12660
                     /locus_tag="OQN13_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(12845..14032)
                     /locus_tag="OQN13_002864"
     CDS             complement(12845..14032)
                     /locus_tag="OQN13_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCS1616167.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(14032..>14369)
                     /locus_tag="OQN13_002865"
     CDS             complement(14032..>14369)
                     /locus_tag="OQN13_002865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616168.1"
                     /translation="KPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCAD
                     LNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVH
                     WPDGVAPPF"
BASE COUNT         3621 a         3606 c         3708 g         3434 t
ORIGIN      
        1 gcccgcgcgc cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta
       61 ctgatacacc ttcgacgcac cgccggttgc cagtaccact gcttttgcgt ggcaggtttc
      121 caccgtttct ttatttcggt tccataccca cgcgcctaca acccgtcgcg tgcccggcag
      181 gccaattttg tcagagacaa tcagatcaac tgcgttgctg cgctccagca cacgaatatt
      241 cggatggttc tgcgccttgc tcaccagcgt ggattgtact tctctaccgg tggcgtcggc
      301 ggcatgaaga atacgacggt gactatgtcc accttcacgg gtcagatggt agctttcttc
      361 gccattcggt tgaacgtggg tatcaaacaa aaccccctgg tcaattaacc attgcacaca
      421 ggaacgagca ttgctggcga caaattcaac tgcatggcga tcgcaaatac cagccccggc
      481 aatcaatgtg tcttccacat gcgagtcaat gctgtcagtt tcatcaaaca cggcggcaat
      541 accgccctgg gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac
      601 ctgatgctgg tcagccaggc gtagcgccag tgaaagtccg gctgcgccgc taccaataat
      661 caacacgtca catgaatgtt cagggagagt attcattttc tttgtttaat ttactaaaca
      721 ttgtttggtc agcatagcat catgttgtgc gaataaacac ctgctatttt aatatttgtt
      781 acagtggcta aacacgttga cgcagggcgg cgagaaaaag agaagttact ggctggtgga
      841 ggattaggtg gtgaaataaa aaggccgttg ggttactctt caggcagtta aatgggcatt
      901 tctacacaga taatgcgatg ttcagattct gtagacttat aatgatagat aatgatccgt
      961 ctacagcatg acaaacaaaa acagatgcgt tacggaactt tacaaaaacg agacactcta
     1021 accctttgct tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt
     1081 ggtttgggga gactttacct cggatgagcg agcagttaac ggaccaggtc ctggttgaac
     1141 gggtccagaa gggagatcag aaagccttta acttactggt agtgcgctat cagcataaag
     1201 tggcgagtct ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag
     1261 cttttattaa agcctatcgt gcgctggatt cgttccgggg agatagcgct ttttatacat
     1321 ggctgtatcg gattgctgta aatacagcga aaaattacct ggttgctcag gggcgtcgtc
     1381 caccttccag tgatgtggat gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga
     1441 aagaaatttc gaaccctgag aacttaatgt tgtcagaaga actgagacag atagttttcc
     1501 gaactattga gtccctcccg gaagatttgc gcatggcaat aaccttgcgg gagctggatg
     1561 gcctgagcta tgaagagata gccgctatca tggattgtcc ggtaggtacg gtgcgttcac
     1621 gtatcttccg agcgagggaa gctattgata acaaagttca accgcttatc aggcgttgac
     1681 gatagcggga tactggataa gggtattagg catgcagaaa gaacaacttt ccgctttaat
     1741 ggatggcgaa acgctggata gtgagctgct taacgaactg gctcataacc cagaaatgca
     1801 gaaaacctgg gaaagctatc acttaatccg tgattcaatg cggggtgata ctcccgaggt
     1861 gctccatttc gatatctctt cacgcgtgat ggccgccatt gaagaagagc cagtacgtca
     1921 accggcgaca ttgatcccgg aagcccagcc tgcgccgcat caatggcaca aaatgccatt
     1981 ctggcagaaa gtacgtccgt gggcggcaca gcttacccaa atgggcgtag cagcatgcgt
     2041 atcgcttgca gttatcgttg gcgtccagca ctataatgga caatctgaaa cgtcccagca
     2101 gcccgaaacg ccggtattta atacactgcc gatgatgggt aaagccagcc cggtaagcct
     2161 gggagtacct tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac aggagcagcg
     2221 tcgtcgcatt aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc actctgaaca
     2281 gcttcagttt gagcaggcgc aaacccagca agccgctgta caggtgccag gacttcaaac
     2341 tttaggaacg caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt
     2401 agcctgttat tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt attacagcag
     2461 atgaacctgg ccagtcagtc actgaattac gagctgtcat tcatcagcat caataaacag
     2521 ggtgttgagt ctctgcgtta tcgacatgca cgcctcgata accgtcctct tgcacaattg
     2581 ttgcaaatgg atggcccgcg ccgggaagtg gtacagcgcg gcaatgaaat cagctatttt
     2641 gaaccgggac ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc tctgccatcg
     2701 cttatctata ccgatttcaa acgcctttct ccttactacg actttatctc cgtcgggcgc
     2761 acgcgtattg ctgatcgtct ttgcgaagtc attcgcgtgg ttgcccgaga tggtacacgc
     2821 tacagctaca tcgtgtggat ggacactgaa tcgaaattac cgatgagggt tgatctcctt
     2881 gatcgcgatg gtgaaacgct ggaacaattt cgcgtgattt cttttaacgt caatcaggat
     2941 atcagcagca gtatgcagac gctggcgaag gcaaatttgc cgccgttgct ttctgttcct
     3001 gtaggtgaaa aagctaaatt cagctggacg ccaacctggt tgccacaggg ttttagcgaa
     3061 gtttccagta gtcgacgtcc gctaccgacg atggacaaca tgcctatcga atcacgtctc
     3121 tattccgacg gattattcag cttctcggta aacgttaacc gcgctacgcc atcgagcacc
     3181 gatcagatgt tgcgcaccgg acgcagaacc gtcagtacaa gcgtacgtga taacgccgaa
     3241 atcaccattg tcggtgaact gccgccgcaa acggcgaaac gcattgccga gaatattaag
     3301 ttcggggcag cgcaatgatc aaagagtggg ctaccgtcgt ctcctggcaa aacgggcagg
     3361 cgctggtcag ttgtgatgtt aaagcctcat gcagcagctg cgcttcacgc gccggttgcg
     3421 gtagccgcgt gttgaataaa cttggcccgc aaaccacgca taccattgtc gtaccctgtg
     3481 atgaaccgtt agtgccgggg caaaaagtgg aattggggat cgccgaaggc agcctgctta
     3541 gctccgcatt actggtttat atgtcgccgc tggtgggatt attccttatc gcttcgctat
     3601 ttcagctact ctttgcttct gacgttgcag cattatgtgg tgcgatcctt ggcggtattg
     3661 gcgggtttct gattgcgcgc ggttactcgc gtaagtttgc tgcccgggca gaatggcaac
     3721 cgatcatatt aagcgtggca ttgccgcctg accttgtgcg atttgaaaca tcttcggaag
     3781 acgcgagcca gtgatttcac tggctttgtt gcattttgca taacaaatgc gaggacgttt
     3841 ccagatacca caatccatac ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat
     3901 gcggcgtttc tattaataca gacgttaagc tcagaacagc gacttctaaa agcctggtta
     3961 accgggcatt aaggcacaat aatcatactt tatgaagaat atacgtaact tttcgatcat
     4021 agctcacatt gaccacggta aatcgacgct gtctgaccgt attatccaga tctgcggtgg
     4081 cctgtctgac cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg agcgtgagcg
     4141 tggcattacc atcaaagcgc aaagcgtgac gctggactac aaagcgtctg acggcgaaac
     4201 ctatcagctt aactttatcg acaccccggg ccacgtagac ttctcctatg aagtttcccg
     4261 ttcgctggct gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg gcgtggaagc
     4321 gcaaaccctg gcaaactgct acaccgccat ggaaatggat ctcgaagtgg tgccagtact
     4381 gaacaagatt gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat
     4441 cgtcggcatc gacgccactg acgcggtgcg ctgttcagcg aaaaccggcg ttggcgtgca
     4501 ggacgttctc gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc cggaaggctc
     4561 gttgcaggca ctaattatcg actcatggtt cgacaactac ctgggcgttg tttcacttat
     4621 ccgtattaaa aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca
     4681 gacctataac gccgaccgtc tgggcatctt cacgccgaaa caggttgacc gcactgaact
     4741 gaaatgtggc gaagtaggct ggctggtatg tgcgattaaa gatatccatg gcgctccagt
     4801 cggcgatacc ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa
     4861 agtcaaaccg caggtatacg ccggtctgtt cccggtaagt tccgacgact atgaagcctt
     4921 ccgtgacgcg ctgggtaaac tcagcctgaa tgatgcctca ctgttctatg agccggaaag
     4981 ctccagcgcg ctgggctttg gtttccgctg cggcttcctt ggcctgctgc acatggagat
     5041 catccaggaa cgtctggaac gtgaatacga tctggatctg atcaccactg cgccgaccgt
     5101 agtgtatgaa gttgaaacca cgtcaaggga agttatctac gtcgacagcc catccaagct
     5161 gcctgcggta aataacattt acgaactgcg cgagccgatt gcagagtgtc acatgctgct
     5221 gccgcaggca tatctcggca acgttattac gctgtgcgta gaaaaacgcg gcgtgcagac
     5281 caatatggtt taccacggta atcaggtggc gctgacgtac gagatcccga tggcggaagt
     5341 ggtgctcgac ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt ctctggatta
     5401 caacttcaaa cgcttccagg cgtccgacat ggtacgtgta gacgtattaa tcaacggtga
     5461 acgtgttgat gcgttggcgc tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga
     5521 gttggtggag aagatgaaag atctgatccc acgccagcag tttgatatcg ccattcaggc
     5581 agcgattggt acgcacatta ttgcgcgatc caccgtgaaa cagctgcgta aaaacgtact
     5641 ggctaaatgt tatggcggcg atatcagccg taagaaaaag ctgctgcaga agcagaaaga
     5701 aggtaagaaa cgcatgaagc agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc
     5761 cattctgcac gtcggcaaag acaacaaata acccttagga gttggcatgg cgaatatgtt
     5821 tgccctgatt ctggtgattg ccacactggt gacgggcatt ttatggtgcg tggataaatt
     5881 ctttttcgca cctaaacggc gggaacgtca ggcagcggcg caggcggctg ccggtgactc
     5941 actggataaa gcaacgttga aaaaggttgc accgaagctt ggctggctgg aaaccggagc
     6001 ttctgttttt ccggtgctgg ctatcgtatt gattgtacgt tcgtttattt atgaaccgtt
     6061 ccagatcccg tcaggttcga tgatgccgac tctgttaatc ggtgatttta ttctggtaga
     6121 gaagtttgct tatggcatta aagatcctat ctaccagaaa acgctgatcg aaaccggtca
     6181 tccgaaacgc ggcgatatcg tggtctttaa atatccggaa gatccaaagc ttgattacat
     6241 caagcgcgcg gtgggtttac cgggcgataa agtcacttac gatccggtct caaaagagct
     6301 gacgattcaa ccgggatgca gttccggcca ggcgtgtgaa aacgcgctgc cggtcaccta
     6361 ctcaaacgtg gaaccgagcg atttcgttca gaccttctca cgccgtaatg gtggggaagc
     6421 gaccagcgga ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc
     6481 cgagcgtaaa gagacactgg gtgatgtgac gcaccgaatt ctgacagtgc cgattgcgca
     6541 ggaccaggtg gggatgtatt accagcagcc agggcaacaa ctggcaacct ggattgttcc
     6601 gccgggacaa tacttcatga tgggcgacaa ccgcgacaac agcgcggaca gccgttactg
     6661 gggctttgtg cctgaagcga atctggtcgg tcgggcaacg gctatctgga tgagtttcga
     6721 taagcaagaa ggcgaatggc cgacaggtgt gcgcttaagt cgcattggcg gcatccatta
     6781 atagcccatc ttcgttcacg ttgtcgccgt tatggcgaca acgtgaatta tttatgagat
     6841 aaatctcccg tggctaacga catcccccgt cgttgtgtat agaatattcc cccgaagttt
     6901 aaggttggca cctccaggtt gccacggcac acgaaacagc gttggtcccc atataccggt
     6961 aaactgaaac tgcagcgaag cagttagcag agccatgtat atcaggtctg tttcgtgtgc
     7021 tgaattgttg acgcatttat ttattggtat cgcatgaacc ccatcgtaat taatcggctt
     7081 caacggaagc tgggctacac ttttaatcat caggaactgt tgcagcaggc attaactcat
     7141 cgtagtgcca gcagcaaaca taacgagcgt ttagaatttt taggcgactc tattctgagc
     7201 tacgttatcg ccaatgcgct ttatcaccgt ttccctcgtg tggatgaagg cgatatgagc
     7261 cggatgcgcg ccacgctggt ccgtggcaat acgctggcgg aactggcgcg cgaatttgag
     7321 ttaggcgagt gcttacgttt agggccaggt gaacttaaaa gcggtggatt tcgtcgtgag
     7381 tcaattctcg ccgataccgt cgaagcatta attggtggcg tattcctcga cagtgatatt
     7441 caaaccgtcg agaaattaat cctcaactgg tatcaaactc gtttggacga aattagccca
     7501 ggcgataaac aaaaagatcc gaaaacgcgc ttgcaagaat atttgcaggg tcgccatctg
     7561 ccgctgccga cttatctggt agtccaggta cgtggcgaag cgcacgatca ggaatttact
     7621 atccactgcc aggtcagcgg cctgagtgaa ccggtggttg gcacaggttc aagccgtcgt
     7681 aaggctgagc aggctgccgc cgaacaggcg ttgaaaaaac tggagctgga atgagcatcg
     7741 ataaaagtta ctgcggattt attgccatcg tcggacgtcc gaacgttggc aaatccacat
     7801 tgttgaacaa actgctgggg cagaagatct ccatcacctc ccgcaaagcg caaacaactc
     7861 gtcaccgcat tgtggggatc catactgaag gcgcgtatca ggcgatctac gttgataccc
     7921 ctggcctgca tatggaagaa aaacgcgcta ttaaccgcct gatgaacaaa gcggcgagca
     7981 gctctattgg cgacgttgag ctggtgattt ttgtcgttga aggcacccgc tggacgccgg
     8041 acgacgaaat ggtgctcaac aaactgcgcg atggcaaagc gccggtgatc ctcgcggtga
     8101 acaaagtgga taacgtgcag gaaaaagccg atctgctgcc gcacttacag ttcctggcaa
     8161 gtcagatgaa cttcctcgat atcgtgccga tctctgctga aaccgggctg aatgtcgaca
     8221 ctatcgcagc aatcgtgcgt aagcatctgc ctgaagcgac ccatcacttc ccggaagatt
     8281 acatcaccga tcgctcacag cgttttatgg cgtctgaaat catccgcgaa aaactgatgc
     8341 gtttcctcgg cgctgaactg ccgtactccg tgaccgtgga gatcgaacgt ttcgtctcta
     8401 acgaacgcgg tggttatgac atcaatggtt tgattctcgt tgagcgtgaa gggcagaaga
     8461 agatggttat tggcaacaaa ggggccaaga tcaaaaccat cgggattgaa gcgcgtaaag
     8521 acatgcagga aatgttcgaa gcgcctgtcc accttgaact gtgggtaaaa gtgaaatccg
     8581 gttgggccga cgacgaacgc gcactgcgca gtctcggtta cgttgacgat ctttaagagt
     8641 cactccgatg gaaggctggc agcgcgcatt tgtcctgcat agtcgcccgt ggagcgaaac
     8701 cagcctgatg ctggacgtct tcacggagga atcgggtcgc gtgcgtctgg ttgccaaagg
     8761 cgcacgctct aaacgctcta ctctgaaagg tgcattacag cctttcaccc ctctcttgct
     8821 acgtttcggc gggcgtggcg aagtcaaaac gctgcgcagt gctgaagctg tctcgctggc
     8881 gctgccatta agcggtatca cgctttacag cggtctgtac atcaacgaac ttctctcccg
     8941 cgtactggaa tacgagacgc gcttctctga actctttttc gattacttgc actgcattca
     9001 gtctcttgca ggtgccactg gtacgccaga acccgcgctg cgccgctttg aactggcact
     9061 gctcgggcat ctgggctatg gcgtcaattt tacccattgc gcgggtagcg gcgagccggt
     9121 agatgacacc atgacgtatc gttatcgcga agaaaaaggg tttatcgcaa gcgtcgttat
     9181 cgacaataaa acgttcaccg gaaggcagtt aaaagcgtta aacgcgcggg aatttcctga
     9241 cgcagacaca ctacgcgccg cgaaacgctt tacccgcatg gcgcttaagc cgtatcttgg
     9301 cggtaaacca ttaaagagca gggaactgtt ccggcagttt atgcctaagc gaacggtgaa
     9361 aacacattat gaatgatgag gattgtcatg gctgaattac tgttaggcgt caacattgac
     9421 catattgcaa cgctgcgtaa cgcacgcggt actgcttacc cggatccggt gcaggcggcg
     9481 tttattgccg agcaggcggg agcggacggc attaccgtgc atttacgtga agatcgccgc
     9541 cacattactg accgcgatgt gcgcatcctg cggcagacgc tggatacccg aatgaatctg
     9601 gagatggcag tgaccgaaga gatgctggcg atcgccgttg agacgaagcc acatttttgc
     9661 tgcctggtac cggaaaagcg tcaggaagta acaaccgaag gcggcctgga cgttgcaggg
     9721 cagcgtgaaa aaatgcgcga tgcctgcaaa cgtctgacag atgccgggat tcagatttct
     9781 ctctttatcg acgccgatga agagcagatc aaagctgcgg cagaggttgg cgcaccgttt
     9841 atcgagatcc acaccggttg ctatgctgat gccaaaactg acgccgaaca ggcgcaagag
     9901 ctggtgcgta tcgccaaagc cgcgaccttt gccgctagtc tcggtctgaa agttaacgcc
     9961 ggacacggtc tgacctatca caacgtgaaa gccattgccg ccatccctga gatgcatgaa
    10021 ctgaatatcg gtcatgccat tattggccgt gcagtgatga ccggactgaa agatgcggtg
    10081 gcagaaatga agcgtctgat gctggaagcg cgtggctaat ggcaatatta ggtttaggca
    10141 cggatatcgt agagatcgct cgcatagaag cagtgatcgc ccgatccggt gagcgcctgg
    10201 cacgccgcgt attaagcgat aacgaatggg caatctggaa aacgcaccac cagccggtgc
    10261 gttttctggc gaagcgtttt gctgtgaaag aagccgcagc aaaagcgttt ggcaccggga
    10321 tccgcaatgg tctggcgttt aatcaatttg aagtattcaa tgatgagctc ggcaaaccac
    10381 ggctacggct atggggtgag gcattaaaac tggcggaaaa gctcggcgtt gtaaatatgc
    10441 atgtaacgct ggcagatgaa cgccactatg cttgtgccac ggtaattatt gaaagttaaa
    10501 ataataccgt tctctttaaa aagtaaaacc cccgaactgg taaggaacgg gggttttact
    10561 ttttaacttt aattttgtgc agcatgcacc ctcaacaaca gcgattatgg atcagtgtac
    10621 ttatctttta caagcaactt gcaatcttta gcataaaaac tcgagccttt acgaagaaag
    10681 caatactgaa cggctgcatt ccttgtttaa ggaaaaacga atgactgatt gccgatacct
    10741 gattaaacgg gtcatcaaaa tcatcattgc tgttttacag ctgatccttc tgttcttata
    10801 acacaaggaa acgtacttaa ggtgcgtccg gtgaaccaat cggacgcgcc tttaataact
    10861 ataaataagt gtcttggcag atactatata aattaactta gtgaatgatt atgctaatgt
    10921 catcaattaa ataaatataa tggcgttaag gcttcccagt aatataatta atactctact
    10981 tccagagtag aatattaaat tttatccgcg tggtgcatca gcacaaattt atcccacaac
    11041 tgttcttctg tctcgacatg cgccggatct ttcacaatag tattggggat cgggcacacc
    11101 ttctggcagg ttggtgtctc gtagtgccct acgcattcgg tacacttatc gctgttaatc
    11161 tcgtagatat gatctcccat tgaaatcgcc tcattcgggc attcgggttc acacatatca
    11221 caattaatac agcgtttagt aattagtaaa gacatttcaa tggattaccg ttaaatcatt
    11281 ttaaaatcag taagttgtat cgagtttgta tgctttactg tcattaactt actgtatgtt
    11341 gatccagtgt atttaacctt gataaactca gtccagcaac acaaaaccgc aacacattgc
    11401 attttgtccc gtagaaaaga cttgtatgtg tgagcttgtt ttctgcgcct acgcagataa
    11461 ggattgagaa tgccgcgcac tgtaacacat aatccggata gccccaataa tgacgatgtt
    11521 ttagccgcat ctgaaaaatg ggacgcctgt aaacccccct ataccagcgc acacatgaaa
    11581 atctgtgttg ctgccgccaa aatcatcctc gctgcttccg gcgtggctcg ccgttccaaa
    11641 tacgaaaaag agaactatct ccgtatcgat ttcagcaaag ccggtaaggt tacattttac
    11701 gccgagtttc caaaaaagat gggcctcaaa ggtaaaaagc tcggcgagtg gccggagctc
    11761 gctatccagc tggcgcgcga aaaagcacta ggtatggctg acggtggact gcgggcagag
    11821 tccgtacatg cagcgctgga aatgtaccgg gatgacctca aagccaaagt agcccggcag
    11881 aagctgagcc cggacagttt cacaacctac ggggtgcgta tcgaccggat taaagcaacg
    11941 ttcggcgagc gtgaggtgtt cagcgacgta acatacaatc ggctggtgga agtgctggac
    12001 gagtggatcg ccactcgctc gaacaataac gccctggagt tgtttgccga gctccgtcgg
    12061 ttctggaagt tctgcgcacc tactctttgc aacggccgca atgttgccgc cagtctgcca
    12121 gatgattatg tttcctcccg cgtacagaaa cctaccccca cacggctttt taccgatatt
    12181 gaatcaatcg cccgactctg gctcaatgtt gctgcctgca cctctgtaca ccagaagaat
    12241 gctgttcgct tcatgatcat cactggtgtt cgtccgatta atgtccataa cctgcgctgg
    12301 gactacgttt acgaggaggc tggtgaaatt gtttatccgg aaggggttat cggcatgcga
    12361 ggggctatga aaacacaaaa ggctttccgc ctgccgataa cgcctgagat ccggcggatt
    12421 atcgacgagc agaaagcctg gcgtgattca gttcctgagt gcaacaggga ttatgtattt
    12481 ttgcagccac gtgatccaat gcagccattt tcaaaacgat cactggataa gctggtgaaa
    12541 acatacagcc cggacggggc tgtaaaagga ataaaacatg atgggactgt taaagggaaa
    12601 gacggtgcat ttaatacgat gtgccgtaaa ttccttaaga gcaatgttat tgccttgatc
    12661 gataggaatt aaaaccccaa aaagattaaa aaaacaccac aaaacggatg tttcttcaac
    12721 accacttttg ctcatatgaa cggaaccgac gattaaactg gatggctctg attgattcag
    12781 ggtatgaatg gcggtttttt gctccgtttc cctcaaaatg gacgcaactt tccctctgcg
    12841 gctctcagcc gcaccaccgc atccgggcca gcggctcatg catcaggacc tgctctgcca
    12901 gacggtagcc ccgcttcagc cccgtaaaac gcatctgact cccgcacagc acgcacttca
    12961 gcgggtcaac cttcagtaac ctctgataca tccctctcca ggtgatttgc atcgccgttt
    13021 ttctcactgt ctccgttatg atgtacacca cttcttccag taaccgccgt ttcgccggac
    13081 tcaaaaaacc gtagtacctc accatacgga accccttatc cgccacatgc caggagaacc
    13141 tttccatgaa ctcatctcca ctcatcaaca ggtattcttc ccgttttgtt cggtgactgt
    13201 tgtaacgcag accgatttca tcctgaccgg cataatgctc cagacgactc atcggcaccg
    13261 gtggcttttt caggtaagag ccaaagtaca ccgccacatg gggggcatta tccatcaccc
    13321 gggatacgtt gacattccag ccacggcggt aatgcgtgtc caggaagcga ttccattccc
    13381 gtttactgct tccttctgct gccagcgcat ccggcatcac caggtcaggg tatttccgtg
    13441 acagcaaccg tgttatccgg tagcgccaca tgctcatcac cttacgggcg taaaaatgaa
    13501 gatttttcca ggtgtggccc gacgtcacac caccggcagt tgtcgataaa tggatatgcg
    13561 gatgccactg ctggtcacgc ccccatgtgt ggatcaccgt gaatatcccc ggctccacat
    13621 ctgcctgatg gcagatttcc agtatcacat ccgctgcaat gcggctcatc tctgtcagta
    13681 accaccggtt gtggaacacc agggaccagt actggcaggg aagtgtgaac acaatatgct
    13741 gccacgggca gtcggggacc aggctcagca gatactgtat ccactgtgcg ccagccttca
    13801 ccccgcagtg cgggcaggag cggcttttac accggaagca gacctttttt gtatggcaac
    13861 agtccggtga tgaacagcac cactgtgtat accccatcag tgtggtcccg cacgccatga
    13921 ttttggtcac cgactcaatc accaccggac gtactgccct ttccggctgc ttctccagcc
    13981 agttaagcca gtggtttccc tgctgaaaga tatcggcaaa acggggaagc atcagaaggg
    14041 cggggcgact ccgtccggcc agtgaaccgt gccacactcc gggcagtaca taccgccggc
    14101 gctgataccg gaaagaatgg tcgcaaattc ccgctccgtg cagcgggcga tttccggata
    14161 cccttcgtca tcaacacgta caaaccagaa gaccagcttt ttgtttcccg catccacaaa
    14221 gaacggaata ttcaggtctg cgcagcattc aacggcatcg tcaaaactat caaagcgcag
    14281 aacttctgcg tcttcttcgt caaaaaaatc atcttcgtga agcttcacga catagcgggg
    14341 aagtttgctt ctttgagagg cgggtttac
//
LOCUS       DAJVRF010000104        14159 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJVRF010000104 DAJVRF010000000
VERSION     DAJVRF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14159
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.104"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..228)
                     /locus_tag="OQN13_002866"
     CDS             complement(<1..228)
                     /locus_tag="OQN13_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HCS1616169.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            385..561
                     /locus_tag="OQN13_002867"
     CDS             385..561
                     /locus_tag="OQN13_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCS1616170.1"
                     /translation="MLNLPIRKLFPTRIASSYGMNFEFMPELKWSFGYPGAIIFMILA
                     GLTPYLYFKRKNWL"
     gene            1187..1486
                     /locus_tag="OQN13_002868"
     CDS             1187..1486
                     /locus_tag="OQN13_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616171.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     ILNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(1533..2420)
                     /gene="rarD"
                     /locus_tag="OQN13_002869"
     CDS             complement(1533..2420)
                     /gene="rarD"
                     /locus_tag="OQN13_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCS1616172.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(2472..2939)
                     /locus_tag="OQN13_002870"
     CDS             complement(2472..2939)
                     /locus_tag="OQN13_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCS1616173.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3104..3973
                     /gene="pldA"
                     /locus_tag="OQN13_002871"
     CDS             3104..3973
                     /gene="pldA"
                     /locus_tag="OQN13_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCS1616174.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            4106..5935
                     /gene="recQ"
                     /locus_tag="OQN13_002872"
     CDS             4106..5935
                     /gene="recQ"
                     /locus_tag="OQN13_002872"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCS1616175.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            5999..6616
                     /gene="rhtC"
                     /locus_tag="OQN13_002873"
     CDS             5999..6616
                     /gene="rhtC"
                     /locus_tag="OQN13_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCS1616176.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(6678..7298)
                     /gene="rhtB"
                     /locus_tag="OQN13_002874"
     CDS             complement(6678..7298)
                     /gene="rhtB"
                     /locus_tag="OQN13_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCS1616177.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7409..8431
                     /gene="pldB"
                     /locus_tag="OQN13_002875"
     CDS             7409..8431
                     /gene="pldB"
                     /locus_tag="OQN13_002875"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCS1616178.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8439..9239
                     /gene="yigL"
                     /locus_tag="OQN13_002876"
     CDS             8439..9239
                     /gene="yigL"
                     /locus_tag="OQN13_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCS1616179.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            9315..10214
                     /gene="bioP"
                     /locus_tag="OQN13_002877"
     CDS             9315..10214
                     /gene="bioP"
                     /locus_tag="OQN13_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCS1616180.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(10102..11055)
                     /gene="metR"
                     /locus_tag="OQN13_002878"
     CDS             complement(10102..11055)
                     /gene="metR"
                     /locus_tag="OQN13_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCS1616181.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            11291..13552
                     /gene="metE"
                     /locus_tag="OQN13_002879"
     CDS             11291..13552
                     /gene="metE"
                     /locus_tag="OQN13_002879"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCS1616182.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(13837..14082)
                     /locus_tag="OQN13_002880"
     CDS             complement(13837..14082)
                     /locus_tag="OQN13_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCS1616183.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3446 a         3424 c         3690 g         3599 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca gaataaaact
      841 caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta atgtatagat
      901 tatgaactga aagcgtgata aacgctcaag cctggatggg tagaatatat ttctgatgga
      961 tgatttgaac atttttacat tgttttatat atataattgt tttgaatata tattacctga
     1021 attatgtggt ttgcatatat atatgcaata tagcagtctt tgcattgata actatacagt
     1081 agtaatttag agtgtgtaag tttttattga tggggtatat tttaattaaa tataattgaa
     1141 atgtattttt attctataaa caatatcttg ggtgaatgga ttctatatgg gaaatggtaa
     1201 cttttggcag ttgttcttgg gtaaagaacc ttacagcaaa aaatctgatt ttgttaaggc
     1261 atggttattg tggtgggcta ttaccatcct tgttctattt attttaagcc tgactacgat
     1321 attaaattta agaagtatag gtatagggtt aagttttggg ttttgtcaaa tccttggtag
     1381 atatacgatg tctctaggat ggagtaagta ctggggagtt ttgggggtat ttcctgtaac
     1441 aaatattgtt ctttttctta tcttaatttt tttcaaaaaa agataaataa gtcagtgggt
     1501 aggctcttta aacggcctac ccatgttaat tcttaacttt ttctacgctg cgtataaatc
     1561 gcatccatca caaaaattgc cagcgccacc caaataaagg cgaaagtcac catcttatcg
     1621 gcacccggtt tttcaccata aaacgtcaca gccagcagga acatcagcgt cgggccaatg
     1681 tactggaaaa agcctaacgt tgagagacgc aagcgcgtgg cagcggcggt aaaacacaac
     1741 agcggtacgg tagtgacaat accggcggcg atcagcagta aattcagcga catcgggttt
     1801 tgccccatat ggctggttga gctgtcggca atagcaaaca ggtaaattgc cgccacgggc
     1861 agcagccaca tggtttcgat taacatgccg gtttgcgctt caacggcaat cttcttgcgt
     1921 accagaccgt agaaggcaaa actaaatgcc agtcccagcg cgataatagg tagcgaacca
     1981 aaagtccaca gctggactaa cacgccacat atcgccagaa tcaccgccag ccattgcatc
     2041 cggcggaatc gctcgccgag gaaaatcatc cccagcacaa tgttcaccag cgggttaata
     2101 aagtaaccaa ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc
     2161 cagttgccac caatcagcac ggcagagact gccagcataa aaattttctg tggcgtctga
     2221 atcagcgttt ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa
     2281 aacgaccaga tcacgcgatg cgtcaggatt tcatcggcgg gcacgtagta aatcaacttg
     2341 aagtacgctg gcgctatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc
     2401 cgcgtttgtt ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg
     2461 cttttatgtc ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct
     2521 tcattgtgta attcaacgcg ggcgacggcg actttattgc ctgcacgcaa caggctactg
     2581 gtagcagtaa aacgctcgcc cctgcctggg cgcagataat caacgcgaag atcaatggtc
     2641 cccatccgcg atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag
     2701 gtacttccca cgcacaccag accggcggcg acatccagcg ccgacgcaat gaccccgccg
     2761 tgcaaaatgc tttgcgccca gttgcccacc atcattggct gatttttaaa ggccagctgt
     2821 gcgaactctt tttcgtaacg ctccagttcc atccccaatg cgcggttaaa tggcatgtga
     2881 taaacaaaca tctcacccac taatttcagg gcttgttcag cggtcagtac ggcagacata
     2941 tcatccttac acttcattgg ttaatgaaat gttgatttta tgcttctttg ttgttggttt
     3001 ctactttagg aagggataac taacggctat ggagttaagt atgtaaaata gcccgcagaa
     3061 aaatattcac cttatcaata attcgttacg gagaacacga ccgatgcgga ctctgcaggg
     3121 ctggttgttg ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa
     3181 agaggtgcat gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca
     3241 tgacaatccg ttcacgctct atccttatga caccaactac ctcatttaca cccaaaccag
     3301 cgatctgaat aaagaagcga ttgccagtta cgactgggcg gaaaatgcgc gtaaggatga
     3361 agtaaagttt cagttgagcc tggcatttcc gctgtggcgt gggattttag gcccgaactc
     3421 ggtgttgggt gcgtcttata cgcaaaaatc ctggtggcaa ctgtccaata gcgaagagtc
     3481 ttcaccgttt cgtgaaacca actacgaacc gcaattgttc ctcggttttg ccaccgatta
     3541 ctgttttgca ggttggacgc tgcgcgatgt ggagatgggg tataaccacg actctaacgg
     3601 gcgttccgac ccgacctccc gcagctggaa ccgcctttat actcgcctga tggcagaaaa
     3661 cggtaactgg ctggtagaag tgaagccgtg gtatgtggtg ggtaatactg acgataaccc
     3721 ggatatcacc aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc
     3781 ggtgttgagt gcgaaaggac agtacaactg gaataccggt tacggcggcg cggagttagg
     3841 cttaagttac ccgatcacca aacatgtgcg cctttatact caggtttaca gcggctatgg
     3901 cgaatcgctc atcgactata acttcaacca gacccgtgtc ggtgtaggag ttatgctaaa
     3961 cgacttgttt tgatgaacgg ttgagtggtt ggcaaatctg gaacccagca tccaggatta
     4021 ccctctcaga gactaaaagc attgcagttt ctcgcgcagg cgctgaaaat agcgcctgtt
     4081 tttatttcag gcaatcgggg ggaatgtggc gcaggcggaa gtgttgaatc tggagtccgg
     4141 agctaaacag gttttacaag aaacctttgg ctaccaacag tttcgccccg gccaggaaga
     4201 aattatcgac actgtgcttt ccggtcgcga ttgcctggtc gtcatgccca ccggtggcgg
     4261 aaaatccctt tgctatcaaa ttcctgcctt attgctaaac ggccttaccg tggttgtttc
     4321 accgctgatt tcgttgatga aagatcaggt ggatcaactg caagccaacg gcgtggcggc
     4381 ggcgtgcctt aactcgacgc aaacccgcga gcagcaactt gaagtgatga caggctgccg
     4441 caccgggcaa attcgtctgc tttatatcgc cccggaacgc ctgatgctgg ataactttct
     4501 tgagcatctg gcgcactgga atccggtgtt attagccgtt gatgaagcgc actgtatctc
     4561 ccaatggggc cacgatttcc gcccggaata tgccgcgctt ggtcagttac gccagcgttt
     4621 cccgaccctg ccgtttatgg cgctgactgc cacagccgac gacaccacgc gccaggatat
     4681 cgtgcgcttg ctggggctga acgatccgct gattcaaatc agcagttttg accgtccgaa
     4741 tattcgctac atgctgatgg agaaattcaa accgctcgat cagttgatgc gctacgtgca
     4801 ggaacagcgc ggtaagtccg gcattatcta ctgcaacagc cgggcgaaag tagaagacac
     4861 cgctgcgcgc ctgcaaagca agggtattag cgcggcggcc tatcatgccg ggctggaaaa
     4921 taatgtccgc gccgacgtgc aggagaaatt ccagcgcgat gacctgcaaa ttgtggtggc
     4981 gacggtggcg ttcggcatgg gcatcaataa accaaacgtt cgcttcgtgg tccactttga
     5041 tattccgcgc aatatcgaat cctattatca ggaaaccggt cgcgccgggc gtgatggcct
     5101 gcctgcggaa gcgatgctgt tttacgatcc ggctgatatg gcgtggctgc gccgttgtct
     5161 ggaagagaag ccgcaggggc agttgcagga tattgagcgc cacaaactca atgcgatggg
     5221 cgcgtttgcc gaagcgcaaa cttgccgtcg tctggtattg ctgaactatt ttggcgaagg
     5281 gcgtcaggag ccgtgcggga actgcgatat ctgcctcgat ccgccgaaac agtacgacgg
     5341 ttcaaccgat gctcagattg ccctttccac cattggtcgt gtgaatcagc ggtttgggat
     5401 gggttatgtg gtggaagtga ttcgtggtgc taataaccag cgtatccgcg actatggtca
     5461 tgacaaactg aaagtctatg gcatgggccg tgataaaagc catgaacatt gggtgagcgt
     5521 gatccgccag ctgattcacc tcggcctggt gacgcaaaat attgcccagc attctgccct
     5581 acaactgaca gaggccgcgc gcccggtgct gcgcggcgaa tcctctttgc aacttgccgt
     5641 gccgcgtatc gtggcgctca aaccgaaagc gatgcagaaa tcgttcggcg gcaactatga
     5701 tcgtaaactg ttcgccaaat tacgcaaact gcgtaaatcg attgccgatg aaagtaatgt
     5761 cccgccgtac gtggtgttta acgacgcaac cttgattgag atggctgaac agatgccgat
     5821 caccgccagc gaaatgctca gcgttaacgg cgttgggatg cgcaagctgg aacgctttgg
     5881 taaaccgttt atggcgctga ttcgtgcgca tgtcgatggc gacgacgaag agtagtcagc
     5941 agcataaaaa agtgccagga tgaagactcc gtaaacgttt cccccgcgag tcaaatgtat
     6001 gttgatgtta tttctcaccg tcgccatggt gcacattgtg gcgcttatga gccccggtcc
     6061 cgatttcttt tttgtctctc agaccgctgt cagtcgttcc cgtaaagaag cgatgatggg
     6121 cgtgctgggc attacctgcg gtgtaatggt ttgggctggg attgcgttgc ttggcctgca
     6181 tttgattatc gaaaaaatgg cctggctgca tacgcttatg gttggcggtg gcctgtatct
     6241 ctgctggatg ggttaccaga tgctgcgcgg cgcgctgaaa aaagaggcgg tttctgcacc
     6301 tgcgccacag gtcgagctgg cgaaaagtgg gcgcagtttc ctgaaaggtt tactgaccaa
     6361 tctcgctaat ccgaaagcga ttatctactt tggctcggtg ttctcattgt ttgtcggtga
     6421 taacgttggc actaccgcgc gctggggcat ttttgcgctg atcattgtcg aaacgctggc
     6481 gtggtttatc gtcgttgcca gcctgtttgc cctgccgcaa atgcgccgtg gttatcaacg
     6541 tctggcgaag tggattgatg gttttgccgg ggcgttattt gccggatttg gcattcattt
     6601 gattatttcg cggtgatgcc agacgcgtct tcagagtaag tcggataagg cgtttacgcc
     6661 gcatccgaca ttatttttca cgcatgcctc gccgatgcta acagcgctcc caccagcata
     6721 aacaacgagc cgaaaatctt attcagcgcc ttcatctgct ttggtccttt aatccataga
     6781 gcaatccgtt gagcaagggt ggcgtaaccg atcatcacaa taatatcgac cacaatagtg
     6841 gtgacgccga gcacgatata ctgcatcagt tgcggctgtt gcggcatgat gaattgcgga
     6901 aatagcgccg ccagaaacac aatacttttg ggattggtga gattcacaaa aactgcgcgc
     6961 tggaacaaat gtcgacgcga ttgagtagag gccagcgatt taaggtcaat tgcaccagcg
     7021 gcgcgccact gctggattcc cagccaaatc aagtaagccg cgcctgccca cttcaacact
     7081 tcaaacgcaa tcactgagcg ggaaaatagc gtccccaacc ccacgccaac cagcacaata
     7141 tgaatcgcca gtccggtctg aagcccagca atagacgcca ccgcgccgcg ataaccgtgg
     7201 ttgagcgagg tggtcatagt gttgattgca ccagagcctg gcgacaggct taaaatgatc
     7261 gatgtcagca ggtaggcaaa ccaccattct aaggtcatga tgaactcccg gtgtgtctat
     7321 ttttgtgcca caatacgcta ctgtcgcagc gttgtgtcag gcacgctaaa aaaaacgatt
     7381 ttacgtggtt taagaggcag attacccgat gtttcagcag caaaaagact gggaaacaag
     7441 agaaaacgcg tttgctgctt ttaccatggg accgctgact gatttctggc gtcagcgtga
     7501 tgaagcagag tttactggtg tggatgacat tccggtgcgc tttgtccgtt ttcgcgcaca
     7561 gcaccatgac cgggtggtag tcatctgccc ggggcgtatt gagagctacg taaaatatgc
     7621 ggaactggcc tatgacctgt tccatttggg gtttgatgtc ttaatcatcg accatcgcgg
     7681 gcagggacgt tccggtcgcc tgttagccga tccgcatctc gggcatgtta atcgctttaa
     7741 tgattatgtt gatgatctgg cggcattctg gcagcaggag gttcagcccg gtccgtggcg
     7801 taaacgctat atactggcac attcgatggg cggtgcgatc tccacattat ttctgcaacg
     7861 ccatccaggt gtatgtgacg ccattgcgct aactgcgcca atgtttggga tcgtgattcg
     7921 tatgccgtca tttatggcac ggcagatcct caactgggcc gaagcgcatc cacgtttccg
     7981 tgatggctat gcaataggca ccgggcgctg gcgcgcgttg ccgtttgcta tcaacgtact
     8041 gacccacagc agacagcgat atcgacgtaa cttacgcttc tatgctgatg acccaacgat
     8101 tcgcgtcggt gggccgacct accattgggt acgcgaaagt attctggctg gcgaacaggt
     8161 gttagccggt gcgggtgatg acgccacgcc aacgcttctc ttgcaggctg aagaggaacg
     8221 cgtggtggat aaccgcatgc atgaccgttt ttgtgaactc cgcaccgccg cgggccatcc
     8281 tgtcgaagga ggacgaccgt tggtaattaa aggtgcttac catgagatcc tttttgaaaa
     8341 ggacgcaatg cgctcagtcg cgctccacgc cattgttgat tttttcaaca ggcataactc
     8401 acccagcgga aaccgctcta cagaggttta aatttcttat gtaccaggtt gttgcgtctg
     8461 atttagatgg cacgttactt tctcccgacc atacgttatc cccttacgcc aaagaaaccc
     8521 tgaagctgct caccgcgcgc ggcatcaact ttgtgtttgc gaccggtcgt caccacgttg
     8581 atgtggggca aattcgcgat aatctggaga tcaagtcgta catgatcacc tccaacggcg
     8641 cgcgcgttca cgatctggat ggcaatctga tttttgctca taacctggat cgcgacattg
     8701 ccagcgatct gtttggcgta gtcaacgaca atctggacat cattactaac gtttatcgcg
     8761 acgacgaatg gtttatgaat cgccatcgcc cggaagagat gcgctttttt aaagaagcgg
     8821 tgttcaaata tgcgctgtat gagcctggat tactggagcc ggaaggcgtc agcaaagtgt
     8881 tcttcacctg cgattcccat gaacaactgc tgccgctgga gcaggcgatt aacgctcgtt
     8941 ggggcgatcg cgtcaacgtt agtttctcta ccttaacctg tctggaagtg atggcgggcg
     9001 gcgtttcaaa aggccatgcg ctggaagcgg tggcgaagaa actgggctac agcctgaagg
     9061 attgtattgc gtttggtgac gggatgaacg atgccgaaat gctgtcgatg gcggggaaag
     9121 gctgcattat gggcagtgcg caccagcgtc tgaaagacct tcatcccgag ctggaagtga
     9181 ttggtactaa tgccgacgac gcggtgccgc attatctgcg taaactctat ttatcgtaat
     9241 cgttctttat ttggtcagtt gtcaacctga tacttcgcta caatggatac tcgttaatca
     9301 aagagttttc cattgtggcg ctacttatca tcaccacgat tctgtgggcc ttctccttta
     9361 gcttttatgg cgagtacctt gcggggcacg tcgatagcta ttttgcggtg ctggtgcgcg
     9421 ttggcctggc ggcactcgtt tttctgccgt ttctgcgtac ccgtggcaat agcctgaaaa
     9481 cggtcggcct gtatatgctg gtgggcgcga tgcagctcgg cgtgatgtat atgctgagtt
     9541 tccgcgctta tctctacctg acggtctccg aattgttgct gttcaccgta ctgacgccgc
     9601 tctacatcac gctgatttat gacatcatga gtaagcgccg tctgcgctgg ggctatgctt
     9661 ttagcgcctt gctggcggtg attggtgccg ggattattcg ctatgatcag gttaccgacc
     9721 atttctggac tggcttgttg ctggtgcaac tctccaatat cacttttgcc atcggcatgg
     9781 tgggttacaa acgcctgatg gaaactcgcc cgatgccgca gcataacgcc tttgcgtggt
     9841 tctatcttgg cgcgttcctg gtggcggtaa ttgcatggtt cttgctggga aatgcgcaga
     9901 aaatgccgca aaccacgttg caatggggca ttctggtgtt tcttggcgtg gtggcttccg
     9961 ggattggcta ctttatgtgg aactacggcg cgacgcaggt ggacgccgga acgctgggca
    10021 ttatgaataa tatgcacgtt ccggcagggc tgctggtaaa cctggctatc tggcaccaac
    10081 agccgcactg gccaacgttc attacaggcg cgctggtgat cctggcctca ctgtgggtgc
    10141 atcgtaagtg ggtcgctccg cgctcttcac aaacggcaga tgatcgcagg cgtgattgcg
    10201 cgctgagcga ataaatgctt ctgtaatcgg ctgacgctgc tcgccatcgc gcacggcggc
    10261 gtacagtcgg ctccacaagc cttcgcccag ggttttggtc accaccagac cttggcgctc
    10321 aaaactctct actacccaat gcggtagcgc ggcaataccc atccgcgcgg caaccatctg
    10381 aatcaacaat aaggtgttat cgacgctttt cagtgacggg ctgacgcctg ccggctgaag
    10441 aaaatgccgc cagacatcca gtcgactacg ctgcaccgga taaatcaaca gcgtctcgct
    10501 ggcgagatct tccggtgtaa ttcgcgtttt cgccgccagt ggatggtcag gtgctaacac
    10561 cagacgcact tcatagtcga acatcggcga ataatgcagg ccactgcgcg gcaggatatc
    10621 ggaggtcatc accagatcca gctcgccctg ttgcaacgca ggctgcgggt caaacgtcac
    10681 gcccgattta aaatccatct ctacctgcgg ccagttctta tggaaatttt ctaacgcggg
    10741 cgtcagccac tgaatacagc tatggcactc aatggcaatg cgcagacgcg tctgctgcgg
    10801 ttcattgcag gcttgcagtg cctggctgat ttgcggcagc acctggtttg ccagttgcaa
    10861 caggatttct ccctgcggtg taaagcgtag cggctggctc ttacgcacaa atagccggaa
    10921 gccaaggcgt tgttccagat cgctaaactg gtgagacagg gcggattgcg tctgatgcaa
    10981 cgtcgccgca gcggctgcga gagagccgca gttccgcaac gcttgtagcg ttttcaggtg
    11041 ttttacttcg atcatgaaag tccttcactt cggcatgaat aatttgcgct tgaggaatat
    11101 acagtaaccg ccaattatgg atgtgtaaac atctggacgg ctaaaatcct tcgtctttta
    11161 aatttatggt gcgttggctg cgtttctcca ccccggtcac ttacttcagt aagctcccgg
    11221 ggatgaataa acttgccgcc ttccctaaat tcaaaatcca taggatttac atataattag
    11281 aggaagaaaa atgacaatat tgaatcacac cctcggtttc cctcgcgttg gcctgcgtcg
    11341 cgagctgaaa aaagcgcaag aaagttattg ggcggggaac tccacgcgtg aagaactgct
    11401 gacagtaggg cgtgaactgc gtgctcgtca ctgggatcaa caaaagcaag cgggtatcga
    11461 cctgctgccg gtgggcgatt ttgcctggta cgatcatgta ctgaccacca gtttgctgct
    11521 gggtaatgtt ccgccacgtc atcagaacaa agacggttcg gtagatatcg acaccctgtt
    11581 ccgtattggt cgtggacgtg cgccgactgg cgaacctgcg gcggcagcgg aaatgaccaa
    11641 atggtttaac accaactatc actacatggt gccggagttc gttaaaggcc aacagttcaa
    11701 actgacctgg acgcagctgc tggaggaagt ggacgaggcg ctggcgctgg gccacaacgt
    11761 taagcctgtg ctgctggggc cggttactta cctgtggctg gggaaagtga aaggtgaaca
    11821 gtttgaccgc cttagcctgc tgaacgacat tctgccggtt tatcagcaag tgctggcaga
    11881 actggcgaaa cgcggcatcg agtgggtaca gattgatgaa cccgcgctgg tactggaact
    11941 accacaggcg tggctggacg catacaaacc cgcttacgac gcgctccagg gacaggtgaa
    12001 actgctgctg accacctatt ttgaaggcgt aacgccaaat ctcgacacga ttactgcgct
    12061 gcctgttcag ggtctgcatg ttgatctcgt acatggtaaa gatgacgttg ctgaactgca
    12121 caagcgcctg ccttctgact ggttgctgtc tgcgggtctg atcaatggtc gtaacgtctg
    12181 gcgcgccgat cttaccgaga aatatgcgca aattaaggac attgtcggca aacgtgattt
    12241 gtgggtggca tcttcctgct cgttgctgca cagccccatc gacctgagcg tggaaacgcg
    12301 tcttgatgca gaagtgaaaa gctggtttgc cttcgcccta caaaaatgcc atgaactagc
    12361 actgctgcgc gatgcgctga acagtggtga cacggcagct ctggcagagt ggagcgcccc
    12421 aattcaggcg cgtcgtcact ctactcgtgt acataatccg gcggtagaaa agcgtctggc
    12481 ggcgatcacc gcccaggaca gccagcgtgc gaatgtctat gaagtgcgtg ctgaagccca
    12541 gcgtgcacgt tttaaactgc cagcgtggcc gaccaccacg attggttcct tcccgcaaac
    12601 cacggaaatt cgtaccctgc gtctggattt caaaaagggc aatctcgacg ccaataacta
    12661 ccgcacgggc attgcggaac atatcaggca ggccattgtt gaacaggaac gtttgggact
    12721 ggatgtgctg gtacatggcg aggccgagcg taatgacatg gtggaatact ttggcgagca
    12781 cctcgacggg tttgtcttta cgcaaaacgg ttgggtacag agctacggtt cccgctgcgt
    12841 gaagccaccg attgttattg gtgacgttag tcgcccggca ccgattaccg tggagtgggc
    12901 gaagtatgcg caatctctga ctgataaacc ggtgaaaggg atgctgactg gcccggtgac
    12961 tattctctgc tggtcgttcc cgcgtgaaga tgtcagccgt gaaaccatcg ccaaacagat
    13021 tgcgctggcg ctgcgtgatg aagtggccga tctggaagcc gctggaattg gcatcatcca
    13081 gattgacgaa ccggctctgc gcgaaggttt accgctgcgt cgtagcgact gggatgcgta
    13141 tctccagtgg ggcgtagagg ccttccgtat caacgccgcc gtggcgaaag atgacacaca
    13201 aatccacact cacatgtgtt attgcgagtt caacgacatc atggattcga ttgcggcact
    13261 ggacgcagac gtcatcacca tcgaaacctc gcgttctgac atggagttgc tggagtcgtt
    13321 cgaagagttt gattatccga atgaaatcgg tccaggcgtc tatgacattc actcgccaaa
    13381 cgtaccgagc gtggaatgga ttgaagcctt gctgaagaaa gcggcaaaac gcattccggc
    13441 agagcgcctg tgggttaacc cggactgtgg cctgaaaacg cgcggctggc cagaaacccg
    13501 cgcggcactg gcgaacatgg tgcaggcggc gcagaatttg cgtcggggat gatgaatatt
    13561 aacgagtcac tgtaaattgc agtacgatga aagaggcaaa actgcctgat actaggccta
    13621 cataatttat gcaatttatt gaatttttgg gttttgttgg ccggacaagg cgttcacgcc
    13681 gcatccggca tgaacaacgc gcactttgcc aataatctga tggcttccat caccggaagc
    13741 cattttgcta tctgcgcccg ttcagataca gaaataaccg aatcattttt acgcaaaata
    13801 taatcctgag cgatttgttc agtaatcaac gctacgttat ttattctcac ttttcacgaa
    13861 attgtatccc ttttcagtaa cgtggtagtg aggcatagac ttatgtccgc tatcctcaat
    13921 aataaccaaa ccctcgttag ctaatttgtt aacaatgtta acaacattac cgaatcctgg
    13981 aatttgcctt atggatagtg tgcgatcgag attcatccag ttccagttta tatctttctc
    14041 agccataatt ttcagtacgt taatttcagt ttcagttatc atagtcaagt gcatcctggt
    14101 ggtataagtc attaagttct cccttaagtt tataatcttc tagagtagca gtatgcgta
//
LOCUS       DAJVRF010000105        14107 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJVRF010000105 DAJVRF010000000
VERSION     DAJVRF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.105"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(13..1371)
                     /gene="hprS"
                     /locus_tag="OQN13_002881"
     CDS             complement(13..1371)
                     /gene="hprS"
                     /locus_tag="OQN13_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCS1616184.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(1371..2042)
                     /gene="hprR"
                     /locus_tag="OQN13_002882"
     CDS             complement(1371..2042)
                     /gene="hprR"
                     /locus_tag="OQN13_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCS1616185.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2175..2588
                     /gene="hiuH"
                     /locus_tag="OQN13_002883"
     CDS             2175..2588
                     /gene="hiuH"
                     /locus_tag="OQN13_002883"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCS1616186.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2697..3701
                     /gene="msrP"
                     /locus_tag="OQN13_002884"
     CDS             2697..3701
                     /gene="msrP"
                     /locus_tag="OQN13_002884"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCS1616187.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            3702..4337
                     /gene="msrQ"
                     /locus_tag="OQN13_002885"
     CDS             3702..4337
                     /gene="msrQ"
                     /locus_tag="OQN13_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCS1616188.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            4594..5244
                     /gene="zinT"
                     /locus_tag="OQN13_002886"
     CDS             4594..5244
                     /gene="zinT"
                     /locus_tag="OQN13_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCS1616189.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            5587..6117
                     /locus_tag="OQN13_002887"
                     /pseudo
     CDS             5587..6117
                     /locus_tag="OQN13_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(6687..6776)
                     /locus_tag="OQN13_002888"
     tRNA            complement(6687..6776)
                     /locus_tag="OQN13_002888"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6740..6742),aa:Ser,seq:cga)
     gene            6870..7667
                     /gene="mtfA"
                     /locus_tag="OQN13_002889"
     CDS             6870..7667
                     /gene="mtfA"
                     /locus_tag="OQN13_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCS1616190.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            7768..7843
                     /locus_tag="OQN13_002890"
     tRNA            7768..7843
                     /locus_tag="OQN13_002890"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7801..7803,aa:Asn,seq:gtt)
     gene            8157..14107
                     /gene="yeeJ"
                     /locus_tag="OQN13_002891"
                     /pseudo
     CDS             8157..14107
                     /gene="yeeJ"
                     /locus_tag="OQN13_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
BASE COUNT         3990 a         3353 c         3171 g         3593 t
ORIGIN      
        1 tgaagcccta tgttaatttc tttgcggtaa cataatccgg aacacattat gcttattgag
       61 atagtgatac gtagcacttc ccccatgtaa ttcggcaatc gctttgacta aagaaagacc
      121 aagtccctga cctacggaat gacgcgaatt atctccccgc caaaatctac ggaagagttt
      181 ttcaggctca tgaattttcg ttccaggact ggcgacatca atattaagat agccgttggt
      241 atcaagaaaa ctggttatat gaatacgcga tttttctggt gaatatctaa tggcattaac
      301 aataagattc gataacattc gttgcagtaa aattttatcc gcaaagattt gctgattgca
      361 ctcgacctta aagcaaatct ctttctcgtc tgaaaggtat tcaagataat ccaacaaatt
      421 ttcgacttcc ttattgagag aaagcgagtc cagtttcacc aaaacattgt ttttatctgc
      481 ccgggcaaga aacagtatgt tctctgttaa ccgcgaaata ttttccagct cttcaatgtt
      541 accggcaatt gttttttgat attcagcgat acttctggtt tgactgagcg taacctgatt
      601 ctgacccagt aatgcattaa tgggcgttct aagttcatga gcgagatcgt cagcaaattg
      661 acttagacgc tcaaaatctt tgactaaagc atgatgcatt ttattcaacg cctgcccaag
      721 aggttttagt tctctcggta atgcgctaac ctcaacaggc tcccggctat cgttataatt
      781 cagcgcttcc gttacaccac tcaacttttt gatctctcgt aatcccgttc tgattaacag
      841 cggacttaat actgagcaaa gtacaatggc gacaatgcaa attataatgc tattaatttt
      901 atactgttca agcatgttat gtctggctga agccaattta gccacagtaa ccgttaatga
      961 tggcgaaact tcatcaatat taattcgtaa agcatcaatc tcagtatcat taataatgct
     1021 tctgtaaatg ccagctgcgc tgattgtctc actagcaggt atgttattta acatgtcatc
     1081 actgacattt gtccggttaa caattttatt gatgccatca ccatgaatga tcaagatatc
     1141 ctgactaaca tccatcatcc ggttaaagta tacaggtaac gtatctggat ttaccccatc
     1201 aattaacaac tgcttgatct gtgctgtccg gttaatgagt gttgtatcat cgcgccattt
     1261 caactcactt gccaggccat tatagagagt ccagacaatt ccagcgccag caacagacag
     1321 cagcaatata aaaagcaagg ttaaacggac ggttatagat aatcttttca ttattttttt
     1381 accgctacga atgaatagcc catcccccgg attgtggcga ttagcttttc aggaaaagga
     1441 tcatcaactt ttgcgcggag cctgcgaatg gcgacatcca ccgtattggt atcactatca
     1501 aagttgattc cccaaatttc actcgcaata accgttctgg gtataatttc gccagctctg
     1561 gaggccagta gccaaagtaa ctgaaactcc ttgcgcgtca gtgtaatact gatattgttc
     1621 ctgctcacac tttgacttac agagtccatt cttaagccac tgatttctaa tgttgaattc
     1681 aaagcgtgat gttgccttaa ttgtgcccga acccttgcca gcaactcaga aaatgaaaaa
     1741 ggttttacca gataatcatt tgctccactg tccagccctc tgactctgtc atcgacagaa
     1801 tcccttgcag taaggcaaat aacaggggtt tgctttgctg ttcttaacgt ttgtaagatc
     1861 tgccagccat ccatacccgg aagcataata tccagaatga tcaatgcata atcatccttc
     1921 agcgcaagat aaagcccatc tctgccatca gaaacggcat caatgacata acccgcttcg
     1981 gaaagcccct gcgttaccca ctcctgggtc ctttgattat cttcaataag taaaatcttc
     2041 atgaaaataa tatgccataa atcatatgtt atagaaacag cctagttcat tacaaaattg
     2101 taatgctgct gtaaggttac cctggccgct ttttcgctat cctcaaaact cattcacatg
     2161 acaaggatat aaacatgtta aagcgttatt tagtactctc cgtagtaacg gcagcatttt
     2221 cattaccttc tttggtttat gccgcacaac aaaacattct tagcgtgcac attttgaacc
     2281 agcaaaccgg aaaacccgct gccgacgtga cagtcactct tgaaaagaaa gcggacaacg
     2341 gctggttaca acttaatacc gccaaaacag ataaggatgg acgaattaag gcactgtggc
     2401 ccgagcaaac tgcaactacg ggcgattacc gtgtcgtatt taaaaccggg gactatttca
     2461 agaaacaaaa tcttgaaagt ttcttccctg agatccccgt tgaatttcat attaataaag
     2521 tgaacgagca ttatcatgtg cctttattac ttagccaata tgggtattca acctatcgtg
     2581 gcagttaatg cataaatatg taaaagcaga tctctgctac tgttagggag gacactccct
     2641 gacagattaa cagtaaacgg ctcttgctgg ctaacgacaa aaaagtgtga tggcttatga
     2701 aaaagaatca atttttaaaa gaatcagatg ttacggccga gtcggtattc tttatgaagc
     2761 gtcgacaggt gttaaaagca ctgggcatca gcgcagctgc actttctttg cctcacgctg
     2821 cgcatgccga tctgcttagc tggtttaaag ggaacgatcg cccgcccgcc cccgccggaa
     2881 aaccgctgga gttcagcaag cctaccgcct ggcaaaataa tctgccactg acgccagtag
     2941 ataaagtctc cggttataac aacttctatg aattcgggct ggataaagcc gatcccgccg
     3001 ctaatgctgg tagcctgaaa accgatccat ggacactgaa aatcagcggc gaagtggcaa
     3061 aaccattgac cctcgatcac gatgatttaa cccgtcgctt cccgctggaa gagcgtattt
     3121 atcgtatgcg ctgcgtggag gcatggtcga tggtggtgcc gtggattggt tttccgctgc
     3181 acaaattgct ggcgcttgcc gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacaa
     3241 tttatgcacc ggaacaaatg ccaggccagc aggaccgctt tatcggcggc gggctgaaat
     3301 atccttatgt cgaaggattg cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg
     3361 tgggtgttta tggcaaggcg ttaccgccac aaaatggcgc gccggtacga ctgattgtgc
     3421 cgtggaaata tggctttaaa gggattaaat cgatagtcag tattaagctg acccgcgagc
     3481 gtccgccaac cacctggaat ctggcagcgc ctgacgaata cggtttttac gccaacgtta
     3541 atccgcatgt tgatcacccg cgctggtcac aggctaccga acgatttatt ggttcaggcg
     3601 gcatcctcga tgttcagcgc cagccaacgc tactgtttaa tggttacgcc gaccaggtgg
     3661 catcgctgta tcgtggcctg gatttgcggg agaatttctg aatgcgtctg acagcaaaac
     3721 aggtgacatg gctgaaagtt agcctgcatc ttgccggatt gttgccgttt ctctggctgg
     3781 tctgggcgat caatcacggt ggactgggtg ccgatccggt gaaagatatt cagcatttta
     3841 ccggtcgcac tgcactgaaa tttttgctgg cgaccttgtt aatcacacct ctggcacgct
     3901 acgcaaaaca gccgttattg atacgcactc gccgcctgtt aggcttatgg tgctttgcct
     3961 gggcgacact gcatttaacc agttacgcat tgctggagct gggcgtgaat aatctggcgt
     4021 tattaggcaa ggagttaatt acccggcctt atttaacgtt aggaattatc agctgggtta
     4081 ttttgcttgc tttagcgttt acttcaaccc aggcgatgca gcgaaaactc ggcaagcact
     4141 ggcaacagtt gcataatttc gtctatcttg tcgcgatcct ggccccaata cattatctgt
     4201 ggtcagtgaa gattatttca ccgcagccgc tcatctacgc tgggctggct gtactgcttt
     4261 tagccttacg gtataagaag ttgcgttccc tttttaagcg gttacgcaag caggttcaca
     4321 acaaattatc tgtataactt catattgccg acaaagtacg cgttatccct gccggatgtg
     4381 gcgcgagcgc cctgtccggc ctgcgcagat cacatcaggt tatgtttcgt ttgtcatcaa
     4441 cctcaaatta tcaacataat tcattttatt tcaataagat agataagtac acctgagaaa
     4501 actatgctct catctcctaa gagttgttgc attttgccat atgttacaat ataacattac
     4561 acatcatata caatgactct ggaggaaact gttttggcaa ttcgtcttca caaactggct
     4621 gttgctttag gtgtctttat tgttagcgct cctgcctttt cgcatggtca tcactcacac
     4681 ggcaaaccct taacagaggt cgaacaaaaa gctgctaatg gtgtttttga tgatgccaat
     4741 gtacaaaacc gaacgctcag tgactgggat ggagtctggc aatccgttta tcctttactg
     4801 caaagtggca aacttgaccc cgtctttcag aagaaagcgg atgcagataa aattaaaaca
     4861 tttgctgaaa ttaaagatta ttatcacaaa ggttatgcaa cagatatcga gatgattggc
     4921 attgaggacg gcattgttga attccataga aataatgaaa caacatcctg taaatatgat
     4981 tacgatggat acaaaatact cacctataaa tcaggcaaga aaggcgttcg ttacttattc
     5041 gaatgtaaag atcctgaaag caaagcccct aaatatatac aatttagcga tcatataatt
     5101 gcaccacgaa aatcatctca ttttcacatt tttatgggta atgattcaca gcaattttta
     5161 ttaaatgaaa tggaaaactg gccaacgtat tatccatatc agttgagtag cgaagaagtg
     5221 gtcgaggaaa tgatgtctca ttgagtattc tcatgataac gcctcgatgc cgctttagta
     5281 agtcatcata actgccactg gtccatccac aaacgccact gaacgcaagc tagctacaga
     5341 cacgctcatc actatgacgt gtctgtatat taataagcta acccgcattg agttaaccaa
     5401 taacggattc catacacaat acggccaaca gagaaagata cctgtgctca cgccattgct
     5461 tatattggct ggttacaatg tgcactatca attttttaaa taacaaattc ttaaaaccac
     5521 atctgtataa ggaattttta aggttcgtgg gatagcttga ctgtaaaaat tacaggagct
     5581 acaaaaatga accgattctc aaaaactcaa atttatttac attggataac gctgcttttc
     5641 gttgcaataa cctatgccgc gatggaactc cgtggctggt ttcctaaagg tagtagtact
     5701 tatctgctga tgcgagaaac acattacaat gcgggtatat tcgtttgggt gttaatgttt
     5761 tcacgcctga ttataaaaca ccgttatagt gatccttcta ttgtaccacc gccacctgcc
     5821 tggcaaatga aagcggcttc gctaatgcac atcatgcttt atataacctt ccttgcactt
     5881 cctctgctgg ggattgcttt gatggcttac agtggaaaat cgtggagttt ccttggtttc
     5941 aatgtgtctc cctttgttac cccaaacagc gaaattaaag cactgataaa aaatattcac
     6001 gaaacctagg caaatatagg ttacttttta atcgcagctc acgctggcgc agcactcttt
     6061 catcactaca ttcagaaaga taatactctg ttacgaatga tgcatcgccg caaataagtc
     6121 gatcccctga caaggaaatg tgaaccatgt acatcaacgc gtcataaatg tttacgcaag
     6181 tgcacaatgc atcctttcta ctatcaacaa ggaggagcag aaactgtgta aatcgtacaa
     6241 tggtaagact tatcaaaaat acaggttaat tttggtattt aaaccttttt tacttgttag
     6301 tgtgatacaa aggctttcaa aaaagctggg aaattcaaca actcttggaa attatggact
     6361 ttgtcccgcg gctttaatct gacggccgcg ttcctttttt gactcaatta ttgacccctt
     6421 ctctacgcaa cttcagttcc caccaccaac tttgcggcag tttcatagga tgaatgtcta
     6481 aaagaataat ggtgagcgat aagaaaacga ctgaataact gcacattttc gctcgaaagc
     6541 ttcctgtcag atccatagcg aatcaagtgc tgaatgtcac agtatcgaac agaaaacagt
     6601 gacgatctaa cccttcaaga atattctaca attgttctgt ttaggaaaag caaggcggga
     6661 agtcaggaga taagtcattg ataaagtggc ggagagaggg ggatttgaac ccccggtaga
     6721 gttgccccta ctccggtttt cgagaccggt ccgttcagcc gctccggcat ctctccgttc
     6781 agatggttgc catgatgcca ggaaatttgg cattttaaca gtccctgttc gtgcaatttt
     6841 gttcaagtga cgagtttgcg agcaaaacga tgattaagtg gccctggaaa gtacaagaat
     6901 cagcacatca aactgccctt ccctggcagg aagcactatc gatccccctt ttaacgggtc
     6961 tgacggaaca ggaacaaagc aaattagtca ctcttgccga acgtttttta cagcaaaagc
     7021 ggcttgttcc tttacagggc tttgaactgg attcattaag aagctgccgg atagcacttc
     7081 tattttgcct acccgttctg gagttaggac tggaatggct ggatggtttt catgaagtct
     7141 taatttatcc tgcgccattt gtggtcgatg atgaatggga agacgatatc ggtctggtgc
     7201 ataaccaacg tattgttcag tcaggtcaga gctggcagca agggcctatc gttttgaact
     7261 ggttggatat acaagattct tttgatgcat ctggttttaa cctgattatt catgaagtcg
     7321 ctcataagct ggacacccgt aacggcgatc gcgccagcgg agttcccttt attccgttgc
     7381 gtgaggttgc tggctgggaa cacgatcttc atgctgcaat gaacaacatt caggaagaaa
     7441 tcgaattggt tggcgagaat gcggcgagca ttgatgctta tgcagccagc gatcctgctg
     7501 aatgttttgc cgtactttct gaatatttct ttagcgcccc agaacttttt gctcctcgtt
     7561 tcccttcatt gtggcaacgt ttctgccaat tttatcaaca agatcctttg cagagactgc
     7621 atcacgctaa tgatacagac tcgttttcgg cgacgaatgt tcattaatta acaactttgc
     7681 agattaatta accaattgaa atgacttatg aaatttagtg ttgacagaca aggtaccgct
     7741 aagtaatatg tgccccgttc acacgattcc tctgtagttc agtcggtaga acggcggact
     7801 gttaatccgt atgtcactgg ttcgagtcca gtcagaggag ccaaattcta aaaattcgct
     7861 tttttagcgc aatgtcactg accttagttg aacattgttt tttaacggat agcgggtttt
     7921 taacacctta agcgccctcg acctttatgg ttgagggcgt tttgctatga acgccatcac
     7981 cattttcccc tcgattataa aacttgagtt attcagtagt ctcccctctt gcaactcaca
     8041 cccaaaactg cctaacgaaa agttattaat tttcaatcgt attgctatca gtatttacat
     8101 tttttcgctg tgctagaaag ggcgcattta tgttagctcg ttcagggaag gtaagcatgg
     8161 ctacgaagaa gagaagtgga gaagaaataa atgaccgaca aatattatgc gggatgggaa
     8221 ttaaactacg ccgcttaact gcgggtatct gtctgataac tcaacttgcg ttccctatgg
     8281 ctgcggcagc acaaggtgtg gtaaacgccg caacccaaca accagttcct gcacaaattg
     8341 ccattgcaaa tgccaatacg gtgccctaca cccttggagc gctggaatcg gcccaaagcg
     8401 ttgccgaacg tttcggtatt tcggtggctg agttacgcaa actcaaccag tttcgtacgt
     8461 ttgctcgagg ttttgataat gtccgccagg gtgatgaact ggatgtcccg gcacaagtta
     8521 gtgaaaaaaa attaaccccg ccgccgggta atagcagtga caacctcgag caacagatag
     8581 ccagtacttc acagcaaatc gggtctctgc tcgccgaaga tatgaacagc gagcaagcgg
     8641 caaatatggc gcgtggatgg gcctcttctc aggcttcagg cgcaatgaca gactggttaa
     8701 gccgcttcgg taccgcaaga atcacgctgg gcgtggatga agattttagc ctgaagaact
     8761 cccagttcga ttttctccat ccgtggtatg aaacgcctga taatctcttt ttcagtcagc
     8821 atactctcca tcgtactgac gagcgtacgc agattaacaa cggcttaggt tggcgtcatt
     8881 tcactcccac atggatgtcg ggcatcaact tctttttcga ccacgatctt agccgttacc
     8941 actcccgcgt cggcattggc gcggagtact ggcgcgacta tctaaaatta agcagtaacg
     9001 gctatttgcg actgaccaac tggcgcagcg cacctgaact ggacaacgat tataaagcac
     9061 gcccggccaa tggctgggat gtacgcgcag aaagctggct acccgcctgg ccgcaccttg
     9121 gcggtaaact ggtctatgaa cagtattatg gcgattaagt ggccctgttc gataaagacg
     9181 atcggcaaag taatcctcat gccataaccg ctggacttaa ctataccccc ttcccgctga
     9241 tgaccttcag cgcggagcaa cgccagggta aacagggcga aaatgacacc cgttttgccg
     9301 tcgattttac ctggcaacct ggcagcgcaa tgcagaaaca gcttgacccg aatgaagtcg
     9361 ctgcacggcg tagccttgca ggcagccgtt atgatctggt ggatcgcaac aacaatatcg
     9421 ttctggaata tcgcaaaaaa gaactggttc gcctaaccct gacagacccc gtgacaggga
     9481 agtcaggaga agtgaaatca ctggtttcgt cgctacaaac caaatatgcc ctgaaaggct
     9541 ataacgtcga agccaccgca ctggaagctg ccggtggcaa agtggtcaca acgggtaaag
     9601 atattctagt taccctgccg gcttaccggt tcaccagtac gccagaaacc gataacacct
     9661 ggccgattga agtcaccgcc gaagatgtca aaggcaattt gtcgaatcgt gaacagagca
     9721 tggtggtcgt tcaggcacct acgctaagcc agaaagattc ctcggtatcg ttaagtaccc
     9781 aaacattgaa cgcggattcc cattcaaccg ccacactgac ttttattgcg catgatgcag
     9841 caggtaatcc tgttgtcggg ctggtgctct cgacgcgtca cgaaggtgtt caggacatca
     9901 ccctttctga ctggaaagat aatggtgacg gaagctatac ccagatcctg accacaggag
     9961 cgatgtctgg cacgctgacg ctgatgccac agctgaatgg tgtggatgcg gctaaagccc
    10021 ccgccgtggt gaatatcatt tctgtttcgt catcccgaac tcactcgtca attaagattg
    10081 ataaggaccg ttatctctcc ggcaatccta tcgaggtgac ggtagaactg agagatgaaa
    10141 atgacaaacc tgttaaggaa caaaaacagc aactgaataa cgcagtcagc atcgacaacg
    10201 tgaaaccagg agtcactaca gactggaaag aaaccgcaga tggcgtctat aaggcgacct
    10261 ataccgccta taccaaaggc agtggactta ctgcgaagct attggaatga agatttgcat
    10321 accgctggat ttatcatcga cgccaacccg cagtcagcga aaattgcgac attatctgcc
    10381 agcaataatg gtgtactcgc caatgagaat gcagcaaaca ccgtctcggt caatgtcgct
    10441 gatgaaggaa gcaacccaat caatgatcat accgtcacgt ttgcggtatt aagcggatcg
    10501 gcaacttcct tcaacaatca aaacaccgca aaaacggatg ttaatggtct ggcgactttt
    10561 gatctgaaaa gtagtaagca ggaagacaac acggttgaag tcacccttga aaatggcgtg
    10621 aaacaaacgt taatcgtcag ttttgtcggc gactcgagta ccgcgcaggt tgatctgcag
    10681 aagtcgaaaa atgaagtggt tgctgacggc aatgacagcg ccacaatgac cgcgaccgtc
    10741 cgggatgcaa aaggcaacct gctcaatgac gtcatggtca ctttcaatgt taattcagca
    10801 gaggcgaaac tgagccaaac cgaagtgaat agccacgacg ggatcgccac agctacgctg
    10861 accagtttga aaaatggtga ttatagggtt acggcctctg tgagctctgg ttcccaggct
    10921 aatcaacagg tgaattttat cggtgatcaa agtactgctg ccctgaccct cagtgtgcct
    10981 tcaggtgata tcaccgtcac caacacagct ccgcaacata tgactgcaac cttgcaggat
    11041 aaaaatggca acccactaaa agataaagaa atcaccttct ctgtgccaaa cgacgtcgca
    11101 agtaagttct cgattagcaa cggaggaaaa ggcatgacgg atagtaacgg ggttgcaatc
    11161 gcctccctga ccggcacgtt agcgggcacg catatgatca tggctcgtct ggctaacagc
    11221 aatgtcagcg atgcacagcc aatgacgttt gtggcggata aagacagagc ggttgtcgtt
    11281 ttgcaaacat cgaaagcgga aatcattggg aatggcgtgg atgagacaac tctgacagca
    11341 acagtgaaag atccgtcgaa tcatccggtg gcggggataa cggtaaactt caccatgcca
    11401 caggacgttg cggcaaactt tacccttgaa aataacggta ttgccatcac tcaggccaat
    11461 ggggaagcgc atgtcacgct gaaaggtaaa aaagcgggca cgcatacggt taccgcaacg
    11521 ctgggtaata acaataccag tgattcgcag ccggtaacat ttgtggcgga caaagcctcg
    11581 gctcaggttg tcctgcagat atcaaaagat gagatcacag gtaatggcgt cgatagcgca
    11641 acgctaactg caacggttaa agatcagttc gacaatgagg tgaataatct tccggtaaca
    11701 ttcagctcag cctcttcagg actcaccctg accccgggag taagtaatac caatgagtct
    11761 ggcatcgcgc aggccactct cgcaggcgtt gcctttggtg agcagacggt cactgcatca
    11821 ctggctaata atggtgccag cgacaacaaa actgtgcatt ttattggcga cacagcggcg
    11881 gcaaaaatta tcgagttgac gcctgtccca gacagcataa tcgccggtac cccgcagaac
    11941 agctccggca gcgccatcac cgccacagtc gttgataata atggctttcc ggtgaaaggt
    12001 gtgactgtga acttcaccag cagaacaaac tctgccgaaa tgacgaatgg cggccaagcc
    12061 gtaacgaacg aacagggtaa ggctaccgtc acttatacca atacccgctc ctcgatagaa
    12121 tcaggagcga gaccggatac cgttgaggcc agtctggaaa atggtagctc cacgcttagc
    12181 acatcaatta atgtcaacgc tgatgcgtct acggcacatc tcacctggct acaggcactt
    12241 tttgatacag tctccgcagg cgagacaacc agtctgtata ttgaggtgaa ggataattac
    12301 ggcaacggtg tcccccagca tcaggtgacc ctcagcgttt caccaagtga aggcgtgacc
    12361 cccagtaata acgctatata tactaccaac cacgacggca atttttacgc aagctttacc
    12421 gctacaaaag ccggggttta tcaagtgacg gcaaccctcg aaaatggcga ttcgatgcaa
    12481 caaacagtga cctatgtgcc gaacgtcacg aatgccgaaa tcacgctggc aacctcgaag
    12541 gatccggtga ttgccgacaa taacgatctc acgacactaa cagcaacagt cgctgataca
    12601 gagggcaatg cgatagccaa cactgaagta acatttactc tgccggaaga tgttaaggcg
    12661 aacttcacgc tgagcgatgg cggtaaagcg attactgatg ctgaaggcaa agcgaaagtc
    12721 acgctgaaag gtacaaaagc aggcgctcat actgttacag catcgataac tggcggtaag
    12781 agtgagcagt tggtggtgaa ctttactgcg gatacgctca ctgcgcaggt taatcttaac
    12841 gttaccgagg acaattttat cgctaataac gtcgggatga ccaggctgca ggcaacagtg
    12901 actgatggaa acggcaaccc gttagccaat gaggcggtga cattcacgct accggcagat
    12961 gtgagcgcaa gctttactct cggacaaggc ggttccgcca ttactgacat caacggcaag
    13021 gctgaagtta cactgagcgg tacaaaatcc ggcacctacc ccgtgacagt tagcgtgaac
    13081 aattatggtg tcagtgatac gaaacaggtg actttgattg ccgatgctgg taccgcaaca
    13141 ctagcctcct taacctctgt atactcattc gtcgtcagca cgaccgaagg cgcgaccatg
    13201 actgcaagcg tcactgacgc taacggcaac ccggtagaag gcataaaagt taatttccgc
    13261 ggaacctccg tcacgctaag cagcaccagc gttgaaacgg atgatcgggg tttcactgaa
    13321 attcttgtga caagcaccga agtcggactg aaaacagttt cagcctctct ggcagataaa
    13381 cctactgaag tcatatcgcg attactgaat gcaaaagcag atattaattc tgcaacgatt
    13441 accagtctgg agatacctga aggtcaggtc atggtcgcac aagacgtagc agttaaagct
    13501 cacgtcaacg accagtttgg caacccgatt cttaatgaat ctgtaacatt cagtgcagag
    13561 ccaccagagc acatgaccat cagccaaaat attgtctcta ctgatacgca tggtatagcc
    13621 gaggtctcca tgacgcccga aagaaacggt tcgtatatgg tgaaagcatc cctggcgaat
    13681 ggagcctcac ttgagaaaca actggaggct attgatgaaa aactgacact ctcggcgtcc
    13741 agcccgctta tcggtgtcaa ttccccaaca ggtgcaactc tgacggcaac gctaacttct
    13801 gcaaatggca ctccagtgga gggtcaggtc atcaacttta gcgtaacgcc agaaggtgcg
    13861 acgttaagtg gcggaaaagt gagaactaac tcttctggtc aggctccagt cgttctgacc
    13921 agcaataaag tcggtacata tacggtgact gcatcgttcc ataacggcgt aacaatacag
    13981 acacagacaa ccgtgaaagt cactggcaac tcaagcaccg cccatgttac tagctttatc
    14041 gctgatccat cgactatcgc cgccaccaac agtgatttaa gtaccttaaa ggcaacggtt
    14101 gaggatg
//
LOCUS       DAJVRF010000106        14091 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJVRF010000106 DAJVRF010000000
VERSION     DAJVRF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14091
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.106"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="OQN13_002892"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="OQN13_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCS1616191.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVEMAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="OQN13_002893"
     CDS             2273..2662
                     /locus_tag="OQN13_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1616192.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="OQN13_002894"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="OQN13_002894"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCS1616193.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="OQN13_002895"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="OQN13_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCS1616194.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="OQN13_002896"
     CDS             complement(4321..4521)
                     /locus_tag="OQN13_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCS1616195.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="OQN13_002897"
     CDS             4687..5232
                     /locus_tag="OQN13_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCS1616196.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="OQN13_002898"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="OQN13_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="OQN13_002899"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="OQN13_002899"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCS1616197.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="OQN13_002900"
     CDS             complement(6529..7353)
                     /locus_tag="OQN13_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCS1616198.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="OQN13_002901"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="OQN13_002901"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCS1616199.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="OQN13_002902"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="OQN13_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCS1616200.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="OQN13_002903"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="OQN13_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCS1616201.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="OQN13_002904"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="OQN13_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCS1616202.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /gene="metI"
                     /locus_tag="OQN13_002905"
     CDS             complement(11316..11969)
                     /gene="metI"
                     /locus_tag="OQN13_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCS1616203.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="OQN13_002906"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="OQN13_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCS1616204.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="OQN13_002907"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="OQN13_002907"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCS1616205.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3433 a         3562 c         3737 g         3359 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagatggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagctgcgtc gcaactgctc
    13981 tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca
    14041 agacgaaaaa tgaataccaa gtctcaagag tgaacacgta attcattacg a
//
LOCUS       DAJVRF010000107        14014 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJVRF010000107 DAJVRF010000000
VERSION     DAJVRF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14014
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.107"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(1..592)
                     /locus_tag="OQN13_002908"
                     /pseudo
     CDS             complement(1..592)
                     /locus_tag="OQN13_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(749..2929)
                     /locus_tag="OQN13_002909"
     CDS             complement(749..2929)
                     /locus_tag="OQN13_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCS1616206.1"
                     /translation="MSKFTVDSFIVELSFSENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLGQLERKFNKTFDKIEQRARSTKAFQFATRFNDTVNPPKQSRQ
                     PRISGNRAITAAHSVNMSKLGDINPLMAKMFKAQYYSLSGKAGNIGSEKFNAELAKLN
                     QSLRETLNKLRSQTKTASINNTSDAFNNLASNAIKLSGAFYSVMGALNAYKAIMNAGL
                     KRDSAQRAAKFVLGDKASEAEVFIRNLADKTGLNISEGLSSYAKFAAGAQGSMSQEQT
                     QELFGNATAMSRLMGLSNDELNGILRAFEQMASKGKIQAEELRGQLGDRMAGAFKLFA
                     EALGMTATELDAAMKNGKVLSADTLPKVAKQMGLMIDKAGGWAEVTKSTQTALGKLAN
                     NWDDTMVKIFSGSQDELNGFLSSLSNLLSEMGMSSSIAGDAIGGLIDMLKAGVDDIRV
                     FNNTIEGWILQTKKFYYSLDDTKRKLLDEVGDGFINFVKGLAIALSAKTLFSATTGVM
                     NLTRAITTLGTRANQVAGQVATGKGGGKLKGVAGGVGSALAIGYADDGYETALALASM
                     IPQIRGVTLGLYALKKALDFMNQEVVKNANMHPSGVGVGSDFNPVYSGDPNKPNMINE
                     GWSRIFSSMGELFNNATMNIARFNHPELNNIKQDSALTSADIQGLRDEISALSKRIQE
                     PVKVSLGGQVVVRPDEPSFFTFNQSANDQFTQDMLLSSSYPEEE"
     gene            complement(3028..3234)
                     /locus_tag="OQN13_002910"
     CDS             complement(3028..3234)
                     /locus_tag="OQN13_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616207.1"
                     /translation="MIDLTERYELIKAISERLKEQPNKPKRIKSFTSSKQKNRIRKIA
                     KWCLDKLPSDCQIVGEDGNYYLVR"
     gene            complement(3259..3969)
                     /locus_tag="OQN13_002911"
     CDS             complement(3259..3969)
                     /locus_tag="OQN13_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1616208.1"
                     /translation="MTNDIYINGKVIDSFSDWSPQTEHPAITIQRKEHDKRIELEQSL
                     KMSPKTTIFVSPEPQEMPDICKSEALLELEKKYYQLLKEQRLRLDDAYSKVTLMQSAI
                     EPSEFEIQDELSQKPFVYYQYEDNDGFGTFQENVAAVISSLPAGHRIVKMVKASRGAG
                     QFIYMTNKSDEELAEIARQNILASRNKQLDNVKLYLSRELQAMKELVKEYEDAKKIAM
                     QADIEQLTKISSKYAKAI"
     gene            complement(4445..4750)
                     /locus_tag="OQN13_002912"
     CDS             complement(4445..4750)
                     /locus_tag="OQN13_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616209.1"
                     /translation="MNYPMQIMVDQETMNWLIDLNRSGFNIYFMHCFEASTYIDKHII
                     NEIKLGDYVHKLEVTDPYNKRFIIEECNRLDLSPEQLLKLNVFLTYQNELNPYSEVM"
     gene            complement(4897..5112)
                     /locus_tag="OQN13_002913"
     CDS             complement(4897..5112)
                     /locus_tag="OQN13_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616210.1"
                     /translation="MKTITVQLQTNKAFRYFENLLELYEGWGSIHGKDDIYLHLSAPN
                     YSLKTPVKQSWLKDYGHQMGLLVSDLS"
     gene            complement(5333..5623)
                     /locus_tag="OQN13_002914"
     CDS             complement(5333..5623)
                     /locus_tag="OQN13_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616211.1"
                     /translation="MTHTVTRLLPEAFERLAAIKAKYPDMKHTALVGEILKESVNLSP
                     FDNVKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADRLEV"
     gene            complement(5947..6243)
                     /locus_tag="OQN13_002915"
     CDS             complement(5947..6243)
                     /locus_tag="OQN13_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616212.1"
                     /translation="MKTYNGERKYRDYLLRRYSISREGYLMKDTHGEVYRIRPKKEGR
                     DYYFVDDVTDLKIDALKFAVLYHYDLWDSVHQLRLKDGDPGNLRATNIIKGKCR"
     gene            complement(6243..7196)
                     /locus_tag="OQN13_002916"
     CDS             complement(6243..7196)
                     /locus_tag="OQN13_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCS1616213.1"
                     /translation="MEKFHMFDDRIYQMTNGVKAADRIIYTVMLNEQKFYHDKGQYYT
                     PSYAHLGQYAGITRTSVIACMKQLEAHGLIKKTNVASNQCVIKVYSLDEVPEVLTELT
                     AKEARKAKLESKVTQSVKKDDPLSQNSLPTESKFFTQSVKIFDTYNNSNNKKELINNN
                     KEDDFEEEYFKKGASLCDSHECDSLGIEEAIKETVKTSDLDDLDSEAAKEVVKKEDKI
                     SDVIASSDHKNTQCPATDDELPDVDLDDEEDPEEELKREQRKAEIKERFRNAKLKNKV
                     SDLDDFDDSDDDFTFTPKKKTHTEPKKEKVKVRRVYGGGMF"
     gene            complement(7579..8241)
                     /locus_tag="OQN13_002917"
     CDS             complement(7579..8241)
                     /locus_tag="OQN13_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCS1616214.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPLLLFKNLHREFGFTLDGAATEHD
                     TLLPRFTNDISRQSWVSEKVFCNLPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNGLTIRSPFPSAVVVFKKEPRKHEITQTVCCGD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKCIADALRMPLSTSYRIAQR
                     LS"
     gene            8757..9326
                     /locus_tag="OQN13_002918"
     CDS             8757..9326
                     /locus_tag="OQN13_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1616215.1"
                     /translation="MRTFLYARVSVDDLTTQNQVEAVKRAGYEVRPSRVIEEVISGAT
                     PAMDRPQFVKLVDKLEEGDCLVVTKIDRLGRDNIDVQKTVTMLLGMGVKLVCLDLPVQ
                     DLSSAEGKLILQLFSTFAEFELNRIRERTREGLERAKAQGKVLGRPVAKNTTEAVLEH
                     KAKGLSQSQVAKLLNLSVRTVSRHWTKTL"
     gene            10054..10227
                     /locus_tag="OQN13_002919"
     CDS             10054..10227
                     /locus_tag="OQN13_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCS1616216.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(10485..11813)
                     /gene="rutG"
                     /locus_tag="OQN13_002920"
     CDS             complement(10485..11813)
                     /gene="rutG"
                     /locus_tag="OQN13_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCS1616217.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(11834..12328)
                     /gene="rutF"
                     /locus_tag="OQN13_002921"
     CDS             complement(11834..12328)
                     /gene="rutF"
                     /locus_tag="OQN13_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCS1616218.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(12339..12929)
                     /locus_tag="OQN13_002922"
     CDS             complement(12339..12929)
                     /locus_tag="OQN13_002922"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCS1616219.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(12939..13739)
                     /gene="rutD"
                     /locus_tag="OQN13_002923"
     CDS             complement(12939..13739)
                     /gene="rutD"
                     /locus_tag="OQN13_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCS1616220.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(13747..>14014)
                     /gene="rutC"
                     /locus_tag="OQN13_002924"
     CDS             complement(13747..>14014)
                     /gene="rutC"
                     /locus_tag="OQN13_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCS1616221.1"
                     /translation="NVLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWK
                     NYAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT         3845 a         3255 c         2896 g         4018 t
ORIGIN      
        1 ccaagcctta ttacatttaa tctgagcatc gagaatatca tttctgatag ctggagcgta
       61 accggataca tcgctaatat tcattaactc tttgcacgat agcataggcg cgttattgcg
      121 ttcagtggtc acgttacctg cattcttctt actggtcaca ccaccgtcat ttgtggtagc
      181 cgttttgcca gtggctgctg aaatatcagt ttgacctacg attgctttaa agttaatttc
      241 ctgaaaagtt agctggatac gaagaccgtt agagatagta acatctttag aggctttaaa
      301 gttggtaata atggaatcgt caattttaat tccatcttta cagataacag aaataatctg
      361 cttctggtca cgccagtttt ccagagtgtc aatgaagttt tctactaatt gaccctgacg
      421 agttaataat aagcttcctt cataattgca tgttctgtgt agtcacctgc atagggctat
      481 caaattccat gttttcagtt gctgataagg ttaagaaggc atctacattg tctcttaagt
      541 ggaaatagat gccatctttg ccactgtaac gaagctgatt agtgtaaatc attaaattct
      601 cctttgattt caaaggctta gtgtatttta atcattttta accaagcctt tgatatatat
      661 gtattttctc tgttaagcgt gagctacgtg aaaaataatt tttggtgtgg gtattaactt
      721 tgctttattc acgtagaaca ccgttaaatc attcttcctc tggatagctt gaacttaaga
      781 gcatatcctg cgtgaactgg tcattagctg attgattgaa agtaaagaaa gatggttcat
      841 ctggtcgaac gacaacttga ccaccaagtg aaactttcac tggctcctga atacgcttac
      901 tcaaagcact aatttcatcc ctcagaccct gaatatctgc actcgttaat gctgaatctt
      961 gcttaatgtt gtttaactct ggatgattga atcttgcaat attcattgtg gcattgttaa
     1021 acaattcacc cattgaacta aaaattctac tccatccttc gttaatcata ttcggtttat
     1081 tagggtcacc agaatacacg ggattaaagt cactacccac acctacccca gacggatgca
     1141 tattagcgtt tttgactact tcctgattca taaaatccag tgctttcttc aatgcatata
     1201 aaccaagagt gacgcctctg atttgtggaa tcattgaagc taatgctaac gctgtttcat
     1261 aaccgtcatc agcataacca attgcaagag cactacctac accaccagct acacctttca
     1321 gttttccacc acctttacca gtagctacct gaccagccac ctgattagct cttgtaccta
     1381 acgttgtgat tgctcttgtc aggttcataa cccctgtagt agcactgaat agcgtctttg
     1441 cagataacgc tatagcaaga cctttgacga agttgataaa accatcaccg acttcatcaa
     1501 gtagttttcg tttagtgtca tcaaggctgt aatagaactt cttagtctgt aaaatccagc
     1561 cttcaatggt gttattgaat acgcgaatat catctacacc agctttaagc atgtcgataa
     1621 gtccaccgat agcatcacca gcgatagaag aactcatccc catttcacta agcaggttac
     1681 tcaggctgga taagaagccg tttagctcat cctgtgaacc tgagaagatt ttcaccatcg
     1741 tatcatccca attgtttgct aacttaccta aagctgtctg agtgctctta gtgacttcag
     1801 cccagccacc cgctttatca atcatcaagc ccatctgttt agcgacttta gggagagtat
     1861 cagcggaaag taccttcccg tttttcatgg ctgcatcaag ctctgtagca gtcataccca
     1921 atgcttcagc gaatagctta aatgcaccag ccatacggtc acctaactgc ccacgtaatt
     1981 cctcagcctg aatcttgccc ttgctcgcca tctgctcaaa tgctctcaaa atgccgttta
     2041 gctcatcgtt agaaagcccc attaagcgac tcatagccgt tgcattacca aatagctcct
     2101 gtgtctgttc ctgtgacata gaaccctgag cacctgctgc aaacttagcg tagcttgata
     2161 gcccttcaga gatattaaga cctgtcttgt ctgctaaatt tcgaataaat acctcagctt
     2221 cagatgcttt atcaccaagt acaaacttag cggctctctg tgcggaatct cgtttaagac
     2281 ctgcattcat aatggccttg taagcgttaa gagcacccat cacagagtaa aatgcaccag
     2341 ataatttaat cgcattgctt gcaagattat taaatgcgtc gcttgtattg ttaatgctgg
     2401 cggtctttgt ctgtgacctg agtttattca acgtttcacg aagagattga tttaattttg
     2461 caagttcagc gttaaacttt tcacttccaa tattaccagc cttaccgctt aagctgtaat
     2521 actgtgcttt aaacatttta gccatcagcg gattaatatc accaagctta gacatattca
     2581 cgctatgcgc tgctgtgatt gctctgtttc cgctaattcg tggttgcctt gattgttttg
     2641 gaggattaac agtgtcatta aatctggtgg caaactgaaa tgcttttgtg cttcttgctc
     2701 tctgctcaat cttgtcaaag gtcttattaa acttcctttc aagctgccct aaagaacttg
     2761 tcagattgct gtcaagttgc ttggtatcga ccttaaacgc tctattcaaa ttgcgttcaa
     2821 ttctttgtgc tgactgcaaa gctgcctttt caactctttg caagccctta ataacgtttt
     2881 cactgaaact aagttcaacg ataaaactat caactgtgaa tttgctcatt catgtttcac
     2941 ctctttaagt gttcatatac gaaaaaggga aagactaccg atatcactcg ataatctctc
     3001 ccgtagattt tcacccaacc aatcttgtta cctaaccaga taataattac catcttcacc
     3061 aacaatctga caatcacttg gtaatttatc aagacaccac ttagctattt tcctgattct
     3121 attcttttgc ttagaggagg tgaatgattt gattcgtttt ggtttgttgg gttgttcttt
     3181 caatcgttca ctaattgctt taataagctc atagcgttct gttaaatcaa tcattcacca
     3241 ccacctttta attattaatt aaatagcctt tgcatatttg ctagaaatct tcgtaagctg
     3301 ctcaatatca gcctgcatag caatcttctt agcatcttca tattctttca caagctcttt
     3361 catagcctga agctctcttg agagatacaa cttaacatta tcaagttgct tgttacgtga
     3421 tgccaagatg ttctgtctag caatttcagc gagttcttca tcgctcttat tagtcatgta
     3481 aataaactga ccagcacctc ttgatgcctt aaccatcttc acaattcgat gtccagcagg
     3541 aagactacta atcacggctg cgacgttttc ttgaaaagta ccaaaaccat cattatcttc
     3601 gtattggtaa tagacaaatg gtttctgtga caattcatcc tgaatctcaa attctgacgg
     3661 ctcaatagct gattgcatca aggtaacttt gctgtaagcg tcatctaatc taaggcgctg
     3721 ttctttcagc agttggtaat atttcttttc aagctcaagt aatgcttcac tcttgcaaat
     3781 atcaggcatc tcttgaggct caggagacac aaagatggtt gtttttggtg acatctttag
     3841 cgattgctca agctcaatac gcttgtcatg ctctttacgc tgaatagtga tagctggatg
     3901 ttcagtctgt ggcgaccagt cactgaagct atcaatcact ttgccattaa tgtaaatatc
     3961 atttgtcatt gcttttcctc ttctaagcgg aagtgtttca gtttttaaaa agcacacctg
     4021 acagcctatc taaacttaga agagacttaa aaggcggttt gccaagtatg ctttttaaaa
     4081 gctgaatagc cctagataat ttcttataaa gggccattca taaggtgaag aaattaaaac
     4141 atctaaagga ttcaaaaagg attcaaacga atgaacaaga gtacttttcg agatgacctc
     4201 tcagactaac ctgaaaggtc atgtagcgaa aagcattgca cctttcagaa taactatcat
     4261 tgatgtagaa tgattaatag tttaattcag gaagagttgc tcttccctat taccatcatc
     4321 tcatgcttaa ggttatttgt caacagcttt ttaacagatt ggtaacatca attcttattt
     4381 accgtattac ctacccagca accaacaaat aaccctaaag tcatccagag aataacgagt
     4441 gtcattacat cacctcgctg taagggttta attcattctg ataggtcaag aatacattca
     4501 gcttcaataa ttgctcaggg cttaaatcaa gtcgattgca ctcttcaatg ataaagcgct
     4561 tattgtatgg gtctgttact tccagcttgt gaacatagtc accaagttta atttcgttga
     4621 ttatatgctt gtcaatatac gttgacgctt caaaacaatg catgaaatag atattaaagc
     4681 ccgacctgtt caggtcaatc aaccaattca tggtttcttg gtcaaccata atttgcattg
     4741 ggtaattcat ttttaaaatc cttcctgtat cgcattctaa gacgttttaa cccctttttt
     4801 agaccactta aggcgattcc ctaagttacc ctgattaggg gcttaaaaac gtttctggtg
     4861 cgtctggtaa agcctgagag atatgttttg ctgtttttag ctcaagtctg agactaatag
     4921 ccccatctgg tgaccgtagt cttttaacca gctttgctta accggagtct tcagagaata
     4981 gttaggggct gacaagtgaa gatagatatc atctttgccg tggatacttc cccatccttc
     5041 gtataactca agcaagtttt caaaataacg aaatgcttta ttagtctgaa gttgtactgt
     5101 gatagttttc atcaagcacc ccattcagaa ataagcttaa ttccctgctc aagctgttca
     5161 gggtcgttat agtcgagtat taccagtggc tttaattcgt gtttaagctg gtggatttga
     5221 gagggtttta atcgtttgtt tgctttgagg gtcacatctt caaattcagc atctgctaaa
     5281 ttctgtctga ttaggtggcg gccaagtgtg gaatattgga acctataaca cattaaacct
     5341 ccaatctatc cgccagttga tggaccttag cgcttaacac ctgaatcatt gagcaacctg
     5401 ctactgcgga ctgtgctttg agtagttcca gttggcccgt tgtgattctg atggccttag
     5461 aaccctctgc gacgccctta acgttatcga aagggctaag gtttaccgac tcttttaaaa
     5521 tctccccaac aagcgctgtg tgcttcatat cgggatattt ggctttgatt gctgccagtc
     5581 gttcaaatgc ctctggcaaa aggcgcgtaa ctgtgtgagt cattgtaaaa atctccttaa
     5641 atctcttact catactacaa tcttaacatc tatttaacat attgtcagta cctcatttac
     5701 atgatactat ggttgtaggg gctgatacag gtgctgtttc ctttcacaaa aggaaaatta
     5761 atgcatgatt ttttcacagc aattattgaa tctcatcgtt ttagctcgtt gggctttgcg
     5821 gtatttattg tgctattggt gttttgcgtt atgaatcgag tcattacaaa aaagacaaag
     5881 cttacaattc gacttaaact tcttcgtgtt gagatagaga tttcagtaaa taaatagctg
     5941 gtggccttac cggcactttc ccttgataat attggtggcc ctcagattac cggggtcacc
     6001 gtcttttaat cgtagctggt ggactgaatc ccataaatcg taatgataca gcaccgcaaa
     6061 cttcaaagcg tcaatcttta aatctgtcac atcatcgacg aaataataat ccctcccttc
     6121 tttcttcggt cgaatccggt aaacttcccc gtgagtatct ttcatcaagt aaccttcacg
     6181 agaaatgctg taacgtctga gtaaatagtc acggtatttt ctctcgccat tataagtttt
     6241 cattaaaaca ttcctccacc ataaacacgt ctgactttaa ctttttcttt ctttggctcg
     6301 gtgtgagttt tcttttttgg tgtaaaagta aaatcatcgt cactatcatc aaagtcatcg
     6361 aggtcactaa ccttgttctt tagcttcgca tttctgaatc tttctttaat ttctgcttta
     6421 cgctgttcac gtttcaattc ctcttcaggg tcttcttcat catcaaggtc aacatccggt
     6481 aattcatcat cagtggcggg gcactgtgtg tttttatggt cactactagc aatcacatca
     6541 gagattttat cttccttttt aacgacctct ttagcagctt cactatcaag gtcatctaag
     6601 tcagatgttt taacagtctc tttaatagcc tcttcaatac caagagaatc acattcatgt
     6661 gaatcacaga gcgaagctcc cttcttaaaa tattcttctt caaagtcatc ttctttattg
     6721 ttattaataa gttctttctt gttattactg ttattatacg tgtcaaagat tttgactgac
     6781 tgagtaaaaa attttgactc agtgggtaaa gaattttgac tcagtgggtc atctttcttg
     6841 actgactgag taactttaga ctccagcttt gcctttctgg cttccttagc tgtaagctct
     6901 gttaaaacct ccggtacttc atcaagtgaa taaactttaa taacacactg attgcttgca
     6961 acgttagttt tcttgattaa accatgtgcc tcaagttgtt tcatacatgc aatcacggat
     7021 gttcttgtga ttccagcata ctgaccaaga tgagcatatg aaggggtata atattgcccc
     7081 ttatcgtgat aaaacttttg ttcattcaac atgacagtgt aaataatacg gtcggcagct
     7141 ttaacgccat tagtcatctg ataaatacgg tcatcaaaca tgtggaattt ttccattata
     7201 ttttcctttt taaaaataaa acaaagaaag ggctgaccac gtatagtgac caaccccaga
     7261 gggagtgaat taattagaag atggactgat aaaggaactc agaagcctga gcgattacgc
     7321 gagtattaac agcaaaagga aaagccttga ttgaataaga ggccgtgaaa atctgtcaat
     7381 accctgacgc ctcccaacgt tgtaacctct taatgttctg agttctttta tcaccccata
     7441 taaaaactat aacagatgtg attataaatt gcaacaatta tttcacagaa taattactga
     7501 ttggtcattt attgttcaca gggatacttc ttaatatctg aatcacctat cttgtaaatg
     7561 accatattta tgagaatttt agctcaacct ttgagcaatc ctgtatgacg tgctcagagg
     7621 cattctgaga gcatctgcaa tgcacttaat gggttttcct tgcctgtata gttttatgaa
     7681 gctgtcgagc gtctcaggtg ggtaaatggt gggtcttcct ctcaagcctc ctctgttaat
     7741 gatggttaac ggtaatagcg tgtcaccaca gcaaaccgtc tgtgtaattt cgtgcttacg
     7801 tggttctttt ttaaaaacaa cgactgcact aggaaacggt gaacgaatag taagcccgtt
     7861 atggcctgct ggtggaaggt atttaactgc acgatgaaga atacggattt catgacagtg
     7921 gttattgctg taaatgtgcc taagccagta gctggtgttg gctctatgcg gtatcaggaa
     7981 agctactaaa tccgcttcag acgcttttaa gaggaacgac ggaatatcgc tgtacggcag
     8041 gttacagaag accttttcac taacccatga ttgacggctt atatcattgg tgaatcgtgg
     8101 taacagcgtg tcatgctcag ttgcagcacc atctagcgta aaaccaaatt cacggtgtaa
     8161 gttcttaaat aacaacaatg gagttctcca gtaatcagaa cccccatgat gtttcccgat
     8221 taaaatatgc tcaattgaca taagaaataa aaaattttat tgcaatcact cttgacaagg
     8281 tttactctct tttaagataa gagatactta acaggctttc taaacggata tcctttctgg
     8341 aatatctatc tcttatctta aaagtcttta cagatacttt aaagatatct gtattaaatc
     8401 tgtacagtaa cttttcagaa tctgaaaatt tttataaaat cgtagtagcc cctcttcaaa
     8461 ttccccttct aaaaatcttc gaaaatatct gacagatgtc tgtagctgct aagtacccgt
     8521 cacaccagct atgtgacccc ctttgcaaaa aagccaaata tcattgactt tctcggtgaa
     8581 ttgctataaa ggcatctatc taggtcttta actgtataaa tacacatgac aacaaacgcc
     8641 cgttaagtgt ctatataaaa aggtagctgt agctatagac aacaattgtg ataattgatt
     8701 taatggctat agacaagcaa gacagacctt tgttgactat atagagagat aagaacatgc
     8761 gtacattcct ttatgctcgt gtatcagttg atgacctcac cacacagaac caagtagaag
     8821 ctgttaaacg tgctggttat gaagtcagac cttcacgagt aattgaggaa gttattagtg
     8881 gggctacacc agctatggac agaccacaat ttgtaaagct ggtggataag ctggaagaag
     8941 gtgattgtct tgtagtgacc aagattgacc gcttaggaag ggataacatt gacgtacaga
     9001 agaccgtcac aatgttactt ggtatgggtg ttaagctggt ctgtctagat ttgcctgtcc
     9061 aagacctttc aagtgctgaa ggtaaactta ttcttcaact attcagtacc tttgcagagt
     9121 ttgaacttaa ccgcatcaga gagcgaacca gagagggctt agaacgagct aaagcacaag
     9181 gtaaggtgtt gggtcgtcct gtagctaaaa acactacaga ggccgtttta gagcataagg
     9241 ctaagggatt gtcacaaagt caggttgcaa agcttctgaa tctctctgtg agaacggtta
     9301 gccgtcactg gacaaagaca ctgtaaaaaa aacttagtag catcttcata gcctccttca
     9361 tgggggcttt tttgtgtctg taacatatgg tgaccaaaca tctatatatc gttatatagc
     9421 cctgtatata gcactatata tagtgtctgg agagggacga ctaacaccct gcatagttcc
     9481 ctgaaaaggg gcttcacaga ttctctatat atttcaataa gttaggataa aaagggacta
     9541 taccgatact gcatagaatc tgtgtaggat tgcatagtca taggggttgg aacaagccca
     9601 actacacctg ataaagcctg ttattaacag gtagttgaat gtacgtgttt agctatgtaa
     9661 aagcaccaga actttagcca tttctaacca ctcctcgtgt tatctctatt ccgtagcgat
     9721 tcgctaccac ttatttaaag ataagacgtc cttttcagag tgcaaatttc acaaccactt
     9781 atttgattta taacaacttt cacaagcacg taattttgtc gcaaaatgac acatttttat
     9841 ctcatcgcgt ttttttaatc ataagagcgg cttatggata attattggag atgatatcta
     9901 ttctcgctaa gaagctgttg caggatatta ccaaacgcgg gtctacccgc gtcagttcac
     9961 taagcttagt cccacgtagc gaaaatatgg cagccgccat acgccgcgtt aattctatgc
    10021 aatatgatgt ctatacccag acggaggtca gtaatggcaa accatcgagg cggttccggc
    10081 aattttgcag aagaccgcga aagagcatca gaagcaggta aaaaaggtgg acagcacagc
    10141 gggggtaatt tcaagaatga cccgcagcgc gcatctgaag caggtaaaaa aggtggcaag
    10201 agcagtcacg gcaaaagcga caactagccg ggctaatcaa tgacgaatgc atttttgtct
    10261 gtagctcgtc aaaaagccat caccgccggt tacccggtgg ttgatactga tgacaaatgt
    10321 aagcttgcct gatgcgcgat gcttatcagg cctaccagaa gattgcaata tattgaattt
    10381 gcactgtttt gtaggccgga taaggcgttt acgccgcatc cggcatgaac aatgcgtacg
    10441 ttgtcaacaa tctgcaccgc cgggtaaccc cggcggtttt ctgtttatgg ctcctgatga
    10501 acaacttctg gcggtggaac gtcaaccaac cttctactta acaacgcatt gagtaaaatc
    10561 gcgccaaagg tggctgtacc gatccctccc aacgtaaaac cgcccagcgt gagagcaaaa
    10621 tcacccgcgc ccagcactaa ggttactgcg accataatca aattaccgtt ctggcttaaa
    10681 tcgacacggt tttgtaccca gatccttgcg cctgcgacgg caatcagccc gaacacaaca
    10741 attgatgcac caccaatcac cgcggccgga atggtatgga tcagcgcgcc aaatttcggc
    10801 gaaaagccca acagcatggc gatgacggca gcagcaacaa acaccagcgt cgagtagact
    10861 tttgtcacgg ccatcacacc gatattttca gcataggtgg tgacgccgct accgccgaca
    10921 gagccggaaa gcatcgttgc cagaccatca ccgacgaatg ctcgccccat atacgggtcc
    10981 atattgcgtc cggtcatccc ggcgaccgcc ttgagatgac ctaagttttc cgccaccaga
    11041 atcaccgcca cgggcgcaat cagcatcatt gcctgactat taaaagcagg agtggaaaaa
    11101 tgtggcaaac cgaaccaggc agcatggctg acgagagtaa aatcgacggc ttttcccagc
    11161 cctaaaacgt tggtcatcac gccatacagc agacaggcga caattaaacc tacgagaatc
    11221 aataaccgct ggatcatgcc acgggtaaac accgccacca gcccaataca cagcaccgtc
    11281 attaccgcca tccagctatc aaaggctgaa gccgatacac ttttcactgc gataggcgct
    11341 aagttcaggc caatcgccat caccaccgca cccgtcacca ccggcggcat tagtcgttca
    11401 atccagcgtg taccaatttt catcaccacc aggccaatga cggtataaac cagcccacag
    11461 gcgataatcc cgcccagtgc gatgctgata ttcgggttaa tgccctgacc gttaaagccc
    11521 gtcgcggcaa tcaccacgcc gacaaaagcc gcgctggagc cgagataact ggggacgcgc
    11581 ccgccggtaa taaagaaaaa cagtaacgtg ccgatccccg acattaaaat ggaaagattg
    11641 ggatccagcc ccatcagaat cggcattaac accgtcgcgc caaacatcgc caccgcgtgt
    11701 tgaacgccca ttactgccgt ctgagcaaac ggcaatcgtt catccggcgc gaccacgccg
    11761 ctctctgtag aggtcgattt taactgccag tgaggaaaac cgaacattgc catcagctgt
    11821 ctccttaagg aggttaacaa gcagggcgca tcagcgcgtg ataactgcga tcgaaccaca
    11881 ccagcccgta gggtgtggtg tgacgatgaa tcgcttcgat ggcgcaaaac agaatgtcgt
    11941 gggtgccgac gctcaccacc tggctgatgc ggcagtcaaa cgaaaccaga gcctcttcca
    12001 gttgcgggca tccggtcaca cccgtctgcc agcgggcggc ggcaaagcgg tgttccatgg
    12061 gcgttttgcc gccaaaaaga tttgaaagcg gctcctgccc ggcgctcagt gtatttacac
    12121 acagcgttcg attttcattg aataccggcc agacggacgc cccacgattc aggcacacca
    12181 gtaatgtggg cggcgtgtcg gtcacgctgc agacggcgct ggcggtgaac ccggcgcgcc
    12241 cggatggacc gtccgtggtg atgatattga ccgccgcgcc catacaggac atcgcatcgc
    12301 gaaaggtttg ttgatcaact atgttcatag tttgctcctt acaacagccc gcaggcttct
    12361 tcaaaggaca gacgtggcag gcgcgcaaaa agtttgctgc tatcgccata gccgatatta
    12421 atcagcagat tgctcttcag cgtgctgccc gcaaaaaagg cgtcgtcaac gtgttgacgg
    12481 tcaaaacccg acatcgggcc ggtatccagt cccagcgcac ggcaagcgac gatcagatag
    12541 gccgcctgca tggagctgtt gcgaaacgct gtttcttcgg caagttgtgg gctggaggta
    12601 aaccaactgc gggcatcacc gtggggaaac agtagtggta accgttcata aaattcactg
    12661 tcccaggcaa cgatagcggt gacgggcgcg gtcagggttt tttgtagatt gccgctggaa
    12721 agcgccgggt gcagacgttc ttttccttct acagtgcgga taaacacgat ccgtgccgga
    12781 gaacagttag ctgatgtcgg cccccatttt atcggggcat aaagctcccg taacgtctca
    12841 tcgctgacgg gtgtctcccg ccagccgttg tgagtgcggg catcggtgaa cagggtgcta
    12901 agcgcacctg ggctaacggc ttcgttcata gcaattcctt acagggcggc ttcacggtga
    12961 tgtaacaggc tggcaagccc gttgagtaac agagcattaa acgtttcggg gtcggtcacg
    13021 ttgcaggcgt gtccgccata gcgcatcacc attttctggc tatcgggcag ggcggcatga
    13081 agctcactgg aacatgctga tggcaccagc agatcatcac tggcgcagat gatttgcacc
    13141 gggcagcgga tgcgatccgc atggtgacta aagtcagcgc gtttgagggc gttaagtcga
    13201 cgcagtaaat tatttttgcc ctgaaaatgc gccagtgcca gcgcgtcttc tgcctcaagg
    13261 cgaggtgcgc gggccgccat ccagtcggcg ggatagagga acaacggctg cgcttccacc
    13321 catgcctgcg cgccgccgct atacagtaat cgttcgcgaa cctgaaaaca gcggcgcgta
    13381 tgggcgttta ttcgtagcca gccgttaacg ctgatcagca cagttaccga cgcgggataa
    13441 tccagcgcca gctgcattcc caccagcgca ccgagcgcat ggccgaccac cgcgtaatgc
    13501 tcaatccctg cggctaccag cgcctgatgc agttccgctg ccatctgggc gatactgtaa
    13561 tcttctgcca gcgtgtcggg attattgccg gtgccgcgct ggtcgtaaca gactacctga
    13621 tactcctgct ccagcaccgc cagttgcggt aaccagtaac tgccgctacc gccaagaccc
    13681 gaaatcaaca ccactacggg cgcatcagca taagggggag gtgagagtga aagtttcatc
    13741 gcggcctcac ttggcgatat gcgcaattgt ggcgatttcc accagcgcgt caggttttac
    13801 cagtccgcac tgaatgcaga atcgcgccgg tttatcaccc ggaaaaaact cggcgtagat
    13861 ttcgttaatc gcggcgtaat ttttccagtc ggtaataaag atgctgttga aggtcacatc
    13921 cgccatcgtg ccacccgccg tctcgatcac cttgcggata gtttccagaa cgtggcgggt
    13981 ttgcgccttt gggtcatcgg caaacagcac gtta
//
LOCUS       DAJVRF010000108        13910 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJVRF010000108 DAJVRF010000000
VERSION     DAJVRF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13910
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.108"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            259..1512
                     /gene="kch"
                     /locus_tag="OQN13_002925"
     CDS             259..1512
                     /gene="kch"
                     /locus_tag="OQN13_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCS1616222.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1567..1740)
                     /locus_tag="OQN13_002926"
     CDS             complement(1567..1740)
                     /locus_tag="OQN13_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCS1616223.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1883..3343
                     /gene="cls"
                     /locus_tag="OQN13_002927"
     CDS             1883..3343
                     /gene="cls"
                     /locus_tag="OQN13_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCS1616224.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3378..3707
                     /locus_tag="OQN13_002928"
     CDS             3378..3707
                     /locus_tag="OQN13_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCS1616225.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDIFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3760..4764)
                     /gene="oppF"
                     /locus_tag="OQN13_002929"
     CDS             complement(3760..4764)
                     /gene="oppF"
                     /locus_tag="OQN13_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCS1616226.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATGGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4761..5774)
                     /gene="oppD"
                     /locus_tag="OQN13_002930"
     CDS             complement(4761..5774)
                     /gene="oppD"
                     /locus_tag="OQN13_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCS1616227.1"
                     /translation="MSVIETATVPLAQQQADVLLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5786..6694)
                     /gene="oppC"
                     /locus_tag="OQN13_002931"
     CDS             complement(5786..6694)
                     /gene="oppC"
                     /locus_tag="OQN13_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCS1616228.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6709..7629)
                     /gene="oppB"
                     /locus_tag="OQN13_002932"
     CDS             complement(6709..7629)
                     /gene="oppB"
                     /locus_tag="OQN13_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCS1616229.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7715..9346)
                     /gene="oppA"
                     /locus_tag="OQN13_002933"
     CDS             complement(7715..9346)
                     /gene="oppA"
                     /locus_tag="OQN13_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCS1616230.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10084..10731)
                     /gene="ychE"
                     /locus_tag="OQN13_002934"
     CDS             complement(10084..10731)
                     /gene="ychE"
                     /locus_tag="OQN13_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCS1616231.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11209..13884
                     /gene="adhE"
                     /locus_tag="OQN13_002935"
                     /gene_synonym="adhC"
     CDS             11209..13884
                     /gene="adhE"
                     /locus_tag="OQN13_002935"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCS1616232.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3661 a         3438 c         3230 g         3581 t
ORIGIN      
        1 ctcaggctac ctcattcctt atagccggtc agtgcattca ctaccacaag cagataataa
       61 ctttatctgt atttctttat atgatgtgag catattcagg aaaaatgacc tttcctgagg
      121 tacagttgct cgctcaaatc ctttgattac tgcggataaa gtcatctact attgtttaac
      181 acattcacgc gctgcatgta tgttcagaaa atgtgcacgc taagataatt aaacaaaatt
      241 gtggttacag aggtatcagt gagtcactgg gctacattca aacaaacagc aacaaattta
      301 tgggtgaccc ttcggcacga cattctcgcg ctggccgtct ttttaaatgg attgcttatt
      361 tttaaaacaa tctatggtat gtcggtcaat ttgcttgata ttttccatat caaagcattt
      421 tcagagttgg atctctcctt gctggcaaac gccccactat ttatgctcgg cgtctttctt
      481 gtcctgaact ccattggctt actgttccgg gcaaagctcg catgggcaat cagtatcatt
      541 ttgttgttga tagcgctaat ttacaccctg catttttatc cctggctgaa atttagtatt
      601 ggattttgca tttttacgct ggtgtttttg ctgatactgc gcaaagactt ctcccacagt
      661 agcgccgcag ccgggacaat ttttgcattt attagtttca cgacattact gttttactca
      721 acctacggtg cgctttattt aagcgaaggt tttaatccgc gaatagaaag tttgatgacc
      781 gcgttctatt tttcgataga aaccatgtca accgtcggct atggcgatat tgtccctgtt
      841 tctgaatcag cacgattgtt cactatttcg gtcattattt ccggcattac cgtttttgcc
      901 acatctatga cctcaatttt tggtccgctt atccgcgggg gattcaacaa acttgtaaaa
      961 ggaaacaatc atacaatgca tcgtaaagat cattttattg tttgcggaca ttcgattctc
     1021 gccatcaata cgatcctgca actgaatcaa cgcggacaaa acgtaacggt tatcagcaac
     1081 ttgcctgaag atgatatcaa gcaacttgag caacgcttag gcgataacgc tgatgttatc
     1141 cccggtgaca gtaatgacag ttcagtatta aagaaagcgg gaatcgatcg ttgccgggcc
     1201 attctggcgc tgagtgataa cgatgcagat aacgcgtttg ttgtactctc ggcaaaagat
     1261 atgagcagtg atgtcaaaac agttctcgcc gtcagtgata gcaaaaacct gaataagatt
     1321 aagatggtac atccggatat cattctctca ccgcaactgt ttggcagcga aattctggcg
     1381 cgagttttaa atggtgaaga gattaataat gatatgctcg tttcaatgtt gttgaactcc
     1441 ggtcatggca ttttcagcga taacgatgaa caagaaacga aagctgacag taaagaatca
     1501 gtgcaaaaat agagtttaaa gacgaaagag taaacttatt gggcaccgca atggtgccca
     1561 gtatatttag atattgtaga tcgcaaacaa taacgagtta cgctgtttgt ttagaatgca
     1621 cttcctgatg ctgtggatac gcatatttcg gcgcgattgc ccctcaagcc aacgcgattt
     1681 acgtcggcta gcctgacgct gcatgcgcca gcgccccact tccgttctac tccgcttcat
     1741 gttaactact ctatgcaata acagaatggt cattataccc ccacccccga cgctgaccag
     1801 tgcttttccc gcgtttttat ttgccagatg aatccatatg cgtaaactca taacaatgcg
     1861 ctttcaaaag gatttctaat ctatgacaac cgtttatacg ttggtgagtt ggttggccat
     1921 tctgggatac tggttgctca ttgcaggcgt aactttacgc attctaatga aacgacgcgc
     1981 agttccctcc gcgatggcct ggctgttgat tatttacatt ctgccgttag tcggaattat
     2041 tgcctatctt gccgttggcg agctccattt aggcaaacgc cgcgctgagc gcgccagagc
     2101 gatgtggcct tccaccgcaa aatggcttaa cgaccttaaa gcctgtaagc atatcttcgc
     2161 cgaagaaaat agcagtgtcg ctgcgccatt attcaagctt tgcgagcgtc gtcaggggat
     2221 cgctggggtc aaagggaatc agctacaact gatgaccgag tcagatgatg tgatgcaggc
     2281 gttaatccgc gacatccagc tcgcgcgcca taatattgag atggtgtttt atatctggca
     2341 gcccggcggc atggcggacc aggtggctga atcattaatg gcggctgcac ggcgtggtat
     2401 tcattgccga ttgatgctcg actccgccgg gagtgtggct tttttccgca gcccgtggcc
     2461 cgagctaatg cgtaatgccg gtattgaagt ggtcgaagcc ttaaaggtca atctgatgcg
     2521 tgtgttttta cgccgtatgg acctgcgcca acatcgcaag atgatcatga tcgataatta
     2581 catcgcgtac accggcagca tgaatatggt cgatcctcgc tacttcaaac aagatgcggg
     2641 cgtagggcaa tggattgatc tgatggcgcg tatggaaggc cccatcgcca ccgcgatggg
     2701 gattatttat tcctgcgact gggagattga aaccggaaaa cgtattctgc cgccaccacc
     2761 agatgtcaat attatgccgt ttgaacaggc cagcggtcac accattcaca caattgcttc
     2821 tggccccggc tttccggaag atctcattca ccaggcatta ttgactgcgg cttattcggc
     2881 gcgtgaatat ttgatcatga caacgcccta ctttgtgcca agcgatgatt tacttcatgc
     2941 gatttgcacg gcggcgcagc gcggggtgga tgtcagtatt atccttccgc gaaaaaatga
     3001 ctcgatgctg gtcggctggg ccagtcgcgc attctttacg gaactgcttg ctgctggggt
     3061 taaaatttat cagtttgaag gcgggttact gcataccaag agcgtgctgg tcgatggcga
     3121 actaagtctg gttggcaccg ttaaccttga tatgcgtagt ctgtggctga atttcgagat
     3181 taccctggca atcgacgata aaggttttgg tgcagacctt gccgccgttc aggacgatta
     3241 tatttcgcgt tcacgtctgc tcgatgcccg tttatggcta aaacgtccat tatggcaacg
     3301 tgtcgccgag cgactgtttt acttcttcag cccgttgctg taaaacgtcg ccaacagagg
     3361 ttaaacaggt agtgactatg gatatggatc taaacaatcg cctgactgaa gatgaaacgc
     3421 ttgagcaggc ctacgatatt tttctcgaac tggctgccga caatctcgac ccagctgatg
     3481 ttctgctgtt caatcttcag tttgaagagc gcggcggcgc ggagttattc gatccggcgg
     3541 aagactggca ggaacatgtt gatttcgatt tgaacccgga cttttttgcc gaagtggtga
     3601 ttggtctggc ggacagtgag gatggtgaaa tcaacgatat tttcgcccgc attctgttat
     3661 gccgcgaaaa agatcacaaa ctttgccata tcatctggcg ggaataaata atcgctttcg
     3721 gcgtgaaaaa gggctgacaa ctgtcagccc ttattgtgtt taaagcggat cgactttcag
     3781 gcaagaaacg gcgtgtctga aactcccttc cagcacagga cgtgttttgg cgcactccgg
     3841 accggcaatc gggcaacagg tacggaaaac acaaccggaa ggcgggttga tcggcgacgg
     3901 taattcccct tccagtaact ggatggtttt gttcttctcc agatccggat caggtatggg
     3961 gactgccgac atcaatgccc tggtgtaagg atgtagtgga ttgtggtaga cctcatcata
     4021 ggtccccagt tctaccgcat ggccgagata catcaccaac acacgatcgg aaatgtgttt
     4081 taccacggcc aggtcatgag cgataaaaat taatgacaat cccatctcac gttgcagctg
     4141 ctggagcagg ttgaccacct gcgcctgaat tgacacgtcc agcgccgaaa tcggctcatc
     4201 gcagataatc agcttcggtt caagaataag agcacgagca atcccgatac gctggcactg
     4261 cccaccggag aactcatgcg gatagcggtt aatcaggtta ggcaataacc cgactttcag
     4321 catcatcgcc ttcacgcgct cgcgaacttc ctggcgtgac attttcggat gataagtacg
     4381 cagtggttca gcgatgatct cgccgatggt catacgcggg tttagcgatg ccaacggatc
     4441 ctggaaaatc atctgaatat cactgcgaac ggcacgccat tcatcgggct tcatgcccag
     4501 caactcttta cctaaccagg caacatgacc gccggtcgct ttaaccaggc caatgatggc
     4561 gcgagcaaag gtggacttac cgcatcccga ttcccctacc acacctaatg tttccccttc
     4621 atacaggcga agcgtgacac catcgacggc tttgagagtt ttcggcggtt gccagaacca
     4681 ctgtttgcca tctttgattt caaagtgcac tttcagatcg gcgatttcga ggaggacttt
     4741 tcttccttcg gttacagcat tcataacagt tcctccaccg gtttaaagca agcacgcaga
     4801 cggccaggcg taaactcttc cagcggcggt gcgctactac aaatttccat cgcatgcgga
     4861 caacgtggct ggaacgggca acctttcggt aatcgcagca ggtttggcgg attaccaggg
     4921 atggtcaaca ttgtttcacc ttccgcatcg agacgcggca ccgcgttgag caaaccgata
     4981 gaataaggat gaacgggttg ataaaagaca tcgcgcgcgt tgccatattc catcgtgcgc
     5041 ccggcgtaca ttaccagcac tttgtcgcag atccccgcca ccacgccaag atcgtgggta
     5101 atcataatga tggcggtatt aaattcccgc ttcagttcat ttaatagcgt catgatctgc
     5161 gcctgtacgg tgacgtccag cgcagtagtg ggttcatccg caatcagcag cttaggtcga
     5221 catagcaatg ccatcgcaat cataactcgc tgacgcatgc caccagaaaa ttcgtgcggg
     5281 tacattttca tgcgtttacg tgcttccggc atttttaccg catcgagcat ccgcaccgac
     5341 tcttcaaacg cctcagcttt gctcatgttc ttatgcagca tcagcacttc catcaactgc
     5401 tcaccgacgc gcatataggg attcaacgaa gtcattgggt cctgaaaaat cattgagatt
     5461 tgttcagcgc gcagtttatt gagttcacgc tctggcaaat tgaggatttc acgcccattg
     5521 aaggttgccg atccgccaat acgtccgttg gcagccaaca ggcccatcaa cgcaaatgca
     5581 gtttgcgatt tacccgaacc agactcacct acaatgccca gcgtttctcc ggcacgtaga
     5641 gaaaaattca aatcattgac cgccgtgaca tcgccgtccg gggtactaaa ggtgacacgc
     5701 aaatctttca cgttcagcag tacgtcagcc tgttgttgtg cgagcggcac agttgcagtt
     5761 tcaattacgc tcatggctgc actccttaac gatctttcgg gtcgagagca tcacgcaagc
     5821 catcgccgat aaagttgaaa caaaacagcg tcaccacgag gaatcccgct gggaacaaca
     5881 ataaccatgg agagacttcc atcgagttcg cgccatcact cagcaatgca ccccagctgc
     5941 ttaacggctc ttgcgtaccc aaccccagga agctaaggaa agattcaaag aggatcatgc
     6001 tgggcaccag aagtgatgcg tagaccacca ccacaccgag tacattcggc acaatgtggc
     6061 gaataacaat gcccgatgtc gatacaccgc caacttgtgc cgcctcaata aactctttgc
     6121 gcttcagact cagggtttgt ccacgcacaa tacgagccat atccagccag gaaaccatgc
     6181 caatcgccac gaaaatcagc aggatgtttt gaccgaaaaa ggtcacaagc aaaatgacga
     6241 agaacatgaa tgggaaggag ttgaggattt ccagcagacg catcattacc gaatccactt
     6301 taccgcccag ataaccggaa agcgaaccgt aaagtgtccc cacgaccact gccaccagtg
     6361 ccgcagcaac accgaccatg agtgagatac gcccgccaat cgcaacgcgc acaagcaggt
     6421 cgcgaccgga tgagtcagta ccaaagtagt gaccggactc catatccggg gcgctggaca
     6481 tcatcgccca gtcagtatcg tcataggcaa actgcgaaag catcggtgcc aggattacaa
     6541 ataacgcgat cagcaccagc actatcagac tggcaaccgc cgcacggtta tgcataaaac
     6601 gtcgacgtgc gtcctgccac aagctgcgcc cttcgacctc cagcttttca ctgaaatttt
     6661 ccagcgtctc gctgtttttc ttacttaaca tcatcgcgag ctccagtatc agtaacggat
     6721 tttcgggtcg ataaccgcat atagcacatc gacaatggca ttaaacaaaa tggttaaagc
     6781 accaaccagg atggtcaggc ttaacactaa ggaatagtca cggttcaatg cgccattaac
     6841 gaacagttgt ccaatccccg gcaaaccata aatggtttcg ataaccatag aaccggtaat
     6901 aatgccgaca aatgcggggc ccatataaga gagcacgggt aacagagcag gttttaatgc
     6961 gtggcgtaaa atgatccgcc gcataggtaa ccctttcgcc cgggcagtac gaataaagtt
     7021 ggagtgtaat acttcaatca tagagcctcg ggtaatacgc gcaatactgg cgatataagc
     7081 gagtgacaac gccaccatcg gcaatatcat gaatttaagc gccccaccat tccagccacc
     7141 gcccggcagc caatgcaaaa tgatcgcaaa tatcatgact aataatggcg caaccacaaa
     7201 actggggata acaaccccgg tcattgccag ccccatcacg gtatagtccc atttggtgtt
     7261 ttgttttaat gcgacaataa cgccagcact aacacccagt attaccgcaa ggaaaaatgc
     7321 ggcggctccc agtttggcag aaacgggaaa actggatgca accaggtcat tgaccgaata
     7381 atctttatat ttaaacgatg gaccgaaatc accgtgcgcc agttgtttca ggtagctgaa
     7441 atactgtgtc atgatcggat cattaagatg atatttcgct tcgatattgg ccatcacttc
     7501 cggcggtaaa gtacgttcgc cggtaaaagg gcttcccggc gcgaggcgca tcataaagaa
     7561 cgaaatagta ataagaataa atagcgtcgg aatcgcttcc agacagcgac gtagaataaa
     7621 ttttaacatt gccctacctt ctggcctgtg ccttctgtaa tgcgataaaa atcagacacc
     7681 gtggagcagg acactcctgc cccacgtatt gccattagtg cttcacaatg tacatattcc
     7741 gggtgtaggt attatccagc ggatctttgc cggtatagcc accaacccac ggtttcacca
     7801 gacgcgcatt cacgtagtaa taaacaggaa caatggccga atccttatcc agctgttgtt
     7861 ctgctttagt gtacagagct gtgcgctgcg cctcgtcagt cactttcagc gtttccgcca
     7921 taatgctgtc aaaggccggg ctcttataat gcgcggtatt catcgagctg ttcgacagca
     7981 tggtgttcag gaaggaagtt ggttcgttgt agtctgcaca ccagcctgca cgggccacat
     8041 caaaagtgcc ctggtgacgg gtgtcgagga acgttttcca ctcctggtta accagtttga
     8101 cgtttacacc aatgtttttc ttccacaatg aagaggcagc aatcgccagc tttttatgca
     8161 gatcagaggt gttatacagc aggttgatgg tcaacggttt gtctgcggta taacccgctt
     8221 cagccagcag ttttttcgct tcttcgttac gtttttcctg gctccagcca aaccattctg
     8281 gctgagtcaa ttttgcgcca tcagtatacg gtggagtgta accataggcg ggcatgtcgc
     8341 cctgcgcttt cactttatta acaatgatat cgcggtccat acctagtttc agtgcggtac
     8401 gcacacgcac atcgttgaat ggcggtttct ggttgttaat ttcgtaatag taagtgcaca
     8461 ggtatggatc aacgtgaact tcgtccggga tctctttttt cagcttctgg aacaattcga
     8521 tcggcatgtt gttatacgtc atgtcgattt caccactacg gtagcggttg acatcggtaa
     8581 cttcagaagc aataggcaaa taggttacct ggttaataac ggttttcgcg ttgttccagt
     8641 aggtcgggct gcgttcaaga acgattcgtt cgtttacgac ccaatctttt aaggtatagg
     8701 caccgttggt gacgatatta ccaggctggg tccatttttc gccgaatttc tcgatagcgg
     8761 cttttggcac cggtgaagtt gatgggtgaa caagtaattt atagaagtac ggaacgggtt
     8821 cacttaaggt gacttctaat gtgtgatcat caatagcttt cacgccgaga tcggtaatcg
     8881 gttttttccc ttcaagaatt tcatcaatac cggcgatatg cccatattgc agataactgg
     8941 catacggaga agcagtgttc ggatcaacag aacgttgcca gctatacaca aagtcttgtg
     9001 ctgtgactgg cgtgctatca gaccatttcg catctttacg caaatggaag gtccagactt
     9061 tcgcgtcttt attatcccag gattcagcga cgccaggtgc tggatgaccg tcaagatcgc
     9121 tgaccagtaa gccttcaaac aggtctcggc tgatattaga ctccggaaca ccttcaattt
     9181 tgtgcggatc taatgactga acttctgaac cattgttacg taccagtgtt tgtttttccg
     9241 ccagtgtgac gcctgcgggt acatcagctg ccagcgcgac attccctgcc attagcgcag
     9301 ccagaacgcc agctgctact aaacttctct tggtgatgtt ggtcattgtt ttttggactc
     9361 cctcattata attactggta ttaaccagca tgtgtaatcc cggattggga ccctgcacag
     9421 gctcggagac tttctgctgt caggttactt tatcgttacg tattactatc accgacttta
     9481 tttattctgg tgattcagac gatcacctta tgtcgattcg tactgtgtct cccctgtcga
     9541 gacacattat tctttcgcga cgctctttaa ttgttaatta ttctcattac caataaagaa
     9601 tcgtctggcg gttgcccgga aagtaccaaa tgcggttctt actcgccaat acattttgca
     9661 aaattgttaa gcaattctct tttattgacc gtatcagaat ctatagcttg catccatgcc
     9721 ttaacaaaca tctttcaatt caatcaaata gagattggct aagcatgtct atcttttctg
     9781 attacagttc cagtagtgaa atgcacaata atttaacaat tgattattat ttagcacttt
     9841 cctctaccaa aggcagtgga attactaata tcatttcaat tattcttcag caagcccagg
     9901 attatgatgt ggcgaaaata acatgatggt gaggagaagc agttaaggaa atccttataa
     9961 ctaactgtaa tttgagaaga atatttttac ctattccttt tgcttttgat aactgcgcgg
    10021 gaacaaagta aaaataatga cgaagtaaac ggagcatggc agctccgttt catttgaaag
    10081 gaattaatta agcaggccgg ggaaaatccc cttaatacca gtaacgataa attcaatccc
    10141 caatgccatc agcaatagcc ccataatacg cgtaatcacg ttgatgccgg tctggcgtaa
    10201 aacccgtacc agccacggtg ccatgcggaa caatccccag caacataaag cgaacaatgc
    10261 aatagccaca aagaaaccaa acagatagct aatgctgtga taacgcgtac cccagacgat
    10321 ggtagaactg atcgcccctg gccccgccat caacggcaac gccaatggca ccacaccaat
    10381 gctttcacgt accgcggttt ctgatttttc ttgcttgttc tgtttatcct cgccaagctt
    10441 gccgctgatc atcgacatcg ctatcgtcac caccaggatg ccccctgcga tacggaacga
    10501 atcaattgat ataccaaaaa gttgcagaat cgtgtcgccg agaaaaagcg agatccacaa
    10561 gataatggcc acagacaggt tggctgtaag gttagttttg tttcgcgctg ccgctgtctg
    10621 ataactggtc atgctgataa agacgggaat aatccctacc gggttgacca gcgcaaataa
    10681 cccgatgaaa aatttgaagt aaacgggaaa atcaaaaaag gtctgaatca cggttagctc
    10741 cgaagcaaaa gccggataat gttagccata aattaaggtt gaaaagacgc gctgacaata
    10801 cgccttttga cagcattttt cacctcctaa ctacttaaaa ttgctatcat tcgttattgt
    10861 tatctagttg tgcaaaacat gctaatgtag ccaccaaatc atactacaat ttattaactg
    10921 ttagctataa tggcgaaaag cgatgctgaa aggtgtcagc ttagcaaaaa tttgatttgg
    10981 atcacgtaat cagtacccag aagtgagtaa tcttgcttac gccacctaga agtgacgcat
    11041 tagagataat aactctaatg tttaaactct tttagtaaat cacagtgagt gtgagcgcga
    11101 gtaagctttt gattttcata ggttaagcaa atcatcaccg cactgactat actctcgtat
    11161 tcgagcagat gatttactaa aaaagtttaa cattatcagg agagcattat ggctgttact
    11221 aatgtcgctg aacttaacgc actcgtagag cgtgtaaaaa aagcccagcg tgaatatgcc
    11281 agtttcactc aagagcaagt agacaaaatc ttccgcgccg ccgctctggc tgctgcagat
    11341 gctcgaatcc cactcgcgaa aatggccgtt gccgaatccg gcatgggtat cgtcgaagat
    11401 aaagtgatca aaaaccactt tgcttctgaa tatatctaca acgcctataa agatgaaaaa
    11461 acctgtggtg ttctgtctga agacgacact tttggtacca tcactatcgc tgaaccaatc
    11521 ggtattattt gcggtatcgt tccgaccact aacccgactt caactgctat cttcaaatcg
    11581 ctgatcagtc tgaagacccg taacgccatt atcttctccc cgcacccgcg tgcaaaagat
    11641 gccaccaaca aagcggctga tatcgttctg caggctgcta tcgctgccgg tgctccgaaa
    11701 gatctgatcg gctggatcga tcaaccttct gttgaactgt ctaacgcact gatgcaccac
    11761 ccagacatca acctgatcct cgcgactggt ggtccgggca tggttaaagc cgcatacagc
    11821 tccggtaaac cagctatcgg tgtaggcgcg ggcaacactc cagttgttat cgatgaaact
    11881 gctgatatca aacgtgcagt tgcatctgta ctgatgtcca aaaccttcga caacggcgta
    11941 atctgtgctt ctgaacagtc tgttgttgtt gttgactctg tttatgacgc tgtacgtgaa
    12001 cgttttgcaa cccacggcgg ctatctgttg cagggtaaag agctgaaagc tgttcaggat
    12061 gttatcctga aaaacggtgc gctgaacgcg gctatcgttg gtcagccagc ctataaaatt
    12121 gctgaactgg caggcttctc tgtaccagaa aacaccaaga ttctgatcgg tgaagtgacc
    12181 gttgttgatg aaagcgaacc gttcgcacat gaaaaactgt ccccgactct ggcaatgtac
    12241 cgcgctaaag atttcgaaga cgcggtagaa aaagcagaga aactggttgc tatgggcggt
    12301 atcggtcata cctcttgcct gtacactgac caggataacc aaccggctcg cgtttcttac
    12361 ttcggtcaga aaatgaaaac ggctcgtatc ctgattaaca ccccagcgtc tcagggtggt
    12421 atcggtgacc tgtataactt caaactcgca ccttccctga ctctgggttg tggttcctgg
    12481 gggggtaact ccatctctga aaacgttggt ccgaaacacc tgatcaacaa gaaaaccgtt
    12541 gctaagcgag ctgaaaacat gttgtggcac aaacttccga aatctatcta cttccgccgt
    12601 ggctccctgc caatcgcgct ggatgaagtg attactgatg gccacaaacg tgcgctcatc
    12661 gtgactgacc gcttcctgtt caacaatggt tatgctgatc agatcacttc cgtactgaaa
    12721 gcagcaggcg ttgaaactga agtcttcttc gaagtagaag cggacccgac cctgagcatc
    12781 gttcgtaaag gtgcagaact ggcaaactcc ttcaaaccag acgtgattat cgcgctgggt
    12841 ggtggttccc cgatggacgc cgcgaagatt atgtgggtta tgtacgaaca tccggaaact
    12901 cacttcgaag agctggcgct gcgctttatg gatatccgta aacgtatcta caagttcccg
    12961 aaaatgggcg tgaaagcgaa aatgatcgct gtcaccacca cttctggtac aggttctgaa
    13021 gtcactccgt ttgcggttgt aactgacgac gctactggtc agaaatatcc gctggcagac
    13081 tatgcgctga ctccggatat ggcgattgtc gacgccaacc tggttatgga catgccgaag
    13141 tccctgtgtg ctttcggtgg tctggacgca gtaactcacg ccatggaagc ttatgtttct
    13201 gtactggcat ctgagttctc tgatggtcag gctctgcagg cactgaaact gctgaaagaa
    13261 tatctgccag cgtcctacca cgaagggtct aaaaatccgg tagcgcgtga acgtgttcac
    13321 agtgcagcaa ctatcgcggg tatcgcattt gcgaacgcct tcctgggtgt atgtcactca
    13381 atggcgcaca aactgggttc ccagttccat attccgcacg gtctggcaaa cgccctgctg
    13441 atttgtaacg ttattcgcta caatgcgaac gacaacccga ccaagcagac tgcattcagc
    13501 cagtatgacc gtccgcaggc tcgccgtcgt tatgctgaaa ttgccgacca cttgggtctg
    13561 agcgcaccgg gcgaccgtac tgctgctaag atcgagaaac tgctggcatg gctggaaacg
    13621 ctgaaagctg aactgggtat tccgaaatct atccgtgaag ctggcgttca ggaagcagac
    13681 ttcctggcga acgtggataa actgtctgaa gatgcattcg atgaccagtg caccggcgct
    13741 aacccgcgtt acccgctgat ctccgagctg aaacagatcc tgctggatac ctactacggt
    13801 cgtgattatg tagaaggtga aactgcagcg aaaaaagaag ccgctccggc taaagctgag
    13861 aaaaaagcga aaaaatccgc ttaatcagta gcgctgtctg gcaatataaa
//
LOCUS       DAJVRF010000109        13797 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJVRF010000109 DAJVRF010000000
VERSION     DAJVRF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.109"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..750)
                     /gene="dsbG"
                     /locus_tag="OQN13_002936"
     CDS             complement(<1..750)
                     /gene="dsbG"
                     /locus_tag="OQN13_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCS1616233.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWILDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGVMTP"
     gene            1122..1685
                     /gene="ahpC"
                     /locus_tag="OQN13_002937"
     CDS             1122..1685
                     /gene="ahpC"
                     /locus_tag="OQN13_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCS1616234.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1856..3421
                     /gene="ahpF"
                     /locus_tag="OQN13_002938"
     CDS             1856..3421
                     /gene="ahpF"
                     /locus_tag="OQN13_002938"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCS1616235.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3542..3970)
                     /gene="uspG"
                     /locus_tag="OQN13_002939"
     CDS             complement(3542..3970)
                     /gene="uspG"
                     /locus_tag="OQN13_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCS1616236.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4191..5429
                     /locus_tag="OQN13_002940"
                     /pseudo
     CDS             4191..5429
                     /locus_tag="OQN13_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            complement(5660..6070)
                     /gene="rnk"
                     /locus_tag="OQN13_002941"
     CDS             complement(5660..6070)
                     /gene="rnk"
                     /locus_tag="OQN13_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCS1616237.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6300..7106)
                     /gene="rna"
                     /locus_tag="OQN13_002942"
     CDS             complement(6300..7106)
                     /gene="rna"
                     /locus_tag="OQN13_002942"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCS1616238.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKNFVIDKVGY"
     gene            complement(7220..8683)
                     /gene="citT"
                     /locus_tag="OQN13_002943"
     CDS             complement(7220..8683)
                     /gene="citT"
                     /locus_tag="OQN13_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCS1616239.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8734..9612)
                     /gene="citG"
                     /locus_tag="OQN13_002944"
     CDS             complement(8734..9612)
                     /gene="citG"
                     /locus_tag="OQN13_002944"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCS1616240.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9587..10138)
                     /gene="citX"
                     /locus_tag="OQN13_002945"
     CDS             complement(9587..10138)
                     /gene="citX"
                     /locus_tag="OQN13_002945"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCS1616241.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10142..11673)
                     /gene="citF"
                     /locus_tag="OQN13_002946"
                     /pseudo
     CDS             complement(10142..11673)
                     /gene="citF"
                     /locus_tag="OQN13_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
     gene            complement(11684..12592)
                     /gene="citE"
                     /locus_tag="OQN13_002947"
     CDS             complement(11684..12592)
                     /gene="citE"
                     /locus_tag="OQN13_002947"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCS1616242.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12589..12885)
                     /gene="citD"
                     /locus_tag="OQN13_002948"
     CDS             complement(12589..12885)
                     /gene="citD"
                     /locus_tag="OQN13_002948"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCS1616243.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12900..>13797)
                     /gene="citC"
                     /locus_tag="OQN13_002949"
     CDS             complement(12900..>13797)
                     /gene="citC"
                     /locus_tag="OQN13_002949"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCS1616244.1"
                     /translation="AGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTK
                     TEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANP
                     FTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEY
                     IISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYN
                     QDMRYWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTL
                     HYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3385 a         3496 c         3672 g         3244 t
ORIGIN      
        1 tggagtcatt acccccataa tgatattaag cgttttctga tcgggcaacc ccacggcctg
       61 ttgcagcgta ttttccttac tcatgtaata gatagccggc gtgacatttg cccccagatc
      121 gtccatcagt ttctcattgt cacttaacac tttcatttgc tctgtactta cgtttgcagg
      181 cacgtttagc ttaagcttgc caccagaggc ttcatattgt tgccaggttt ttgcgggatc
      241 tttggaggca agaattgccg ctgctgtcgc cgggctttct ggcttgataa ccccaaccaa
      301 caatgttctt aattgcactt tgccagaatc tacccacggg cgcgcctgct gccagaactg
      361 tttacaatat gggcagaacg gatcggcgaa gacgtagaca atgaccggcg catctttttt
      421 accgtcgagg atccagtggg attgttccat ccgttgccac atttcgcgtc cggctggtgc
      481 gtaaatttct ttttcgataa gtgtgttact caggttttca cctttctcgt tgtacatgta
      541 accagagata gcgtgcttac catcgggagt caggtagatg gtgacgccca tatcctgata
      601 ctttccgaga taacctttca ttcctccggg ggcatcgaac gttttgatga ttgtaatgcc
      661 ctgtttttca atcgctttta ctggggaagg aagttcctct gcgaaggcga ttgcaggaag
      721 cagagccagt aaaagtatct tttttaacat taatttgtcc ttttcagtca gtgcaaaagt
      781 cgagtaaaag gcataaccta tcactgtcat aggtaagagc ttagatcagg tgattgccct
      841 ttgtttatga gggtgttgta atccatgtcg ttgttgcatt tgtaagggca acacctcagc
      901 ctgcaggcag gcgctgaaga taccaaaggg tagttcagat tacacggtca cctggaaagg
      961 gggccatttt actttttatc gccgctggcg gtgcaaagtt cacaaagttg tcttacgaag
     1021 gttgtaaggt aaaacttatc gatttgataa tggaaacgca ttagccgaat cggcaaaaat
     1081 tggttacctt acatctcatc gaaaacacgg aggaagtata gatgtccttg attaacacca
     1141 aaattaaacc ttttaaaaac caggcattca aaaacggcga attcatcgaa atcaccgaaa
     1201 aagataccga aggccgctgg agcgtcttct tcttctaccc ggctgacttt actttcgtat
     1261 gcccgaccga actgggtgac gttgctgacc actacgaaga actgcagaaa ctgggcgtag
     1321 acgtatacgc agtatctacc gatactcact tcacccacaa agcatggcac agcagctctg
     1381 aaaccatcgc taaaatcaaa tatgcgatga tcggcgaccc gactggcgcc ctgacccgta
     1441 acttcgacaa catgcgtgaa gatgaaggtc tggctgaccg tgcgaccttc gttgttgacc
     1501 cgcagggtat catccaggca atcgaagtta ccgctgaagg cattggccgt gacgcgtctg
     1561 atctgctgcg taaaatcaaa gcagctcagt acgtagcttc tcacccaggt gaagtttgcc
     1621 cggctaaatg gaaagaaggt gaagcaactc tggctccgtc tctggacctg gttggtaaaa
     1681 tctaaatttc cttcgtcttt cgcgctacca gctccggcgc gaaatacttc ggaaattcac
     1741 ctaattcttc gggtgctgcg gcacccgatt ttcttccccg caccatgatg caagctgcat
     1801 ccaggtagcc gtagaggccg cttgcatgat gatgtttaaa gcccaggaga taaacatgct
     1861 cgacacaaat atgaaaactc aactcaaggc ttaccttgag aaattgacca agcctgttga
     1921 gttaattgcc acgctggatg acagcgctaa atcggcagaa atcaaggaac tgttggctga
     1981 aatcgcagaa ctgtcagaca aagtcacctt taaagaagat aacagcttgc cggtgcgtaa
     2041 gccgtctttc ctgatcacca acccaggttc caaccagggg ccacgttttg caggctcccc
     2101 gctgggccac gagttcacct cgctggtact ggcgttgctg tggaccggtg gtcatccgtc
     2161 gaaagaagcg cagtctctgc tggagcagat tcgccatatt gacggtgatt ttgaattcga
     2221 aacctattac tcgctctctt gccataactg cccggacgtg gtgcaggcgc tgaacctgat
     2281 gagcgtactg aacccgcgca tcaagcacac tgcaattgac ggcggcacct tccagaacga
     2341 aatcaccgat cgcaacgtga tgggcgttcc ggcagtgttc gtaaacggga aagagtttgg
     2401 tcagggccgc atgacgttga ctgaaatcgt tgccaaaatt gatacgggcg cggaaaaacg
     2461 tgcggcagaa gagctgaaca agcgtgatgc ttatgacgta ttaatcgtcg gttccggccc
     2521 ggcgggtgca gcggcagcaa tttactccgc acgtaaaggc atccgtaccg gtctgatggg
     2581 cgaacgtttt ggtggtcaga tcctcgatac cgttgatatc gaaaactaca tttctgtacc
     2641 gaagactgaa gggcagaagc tggcaggcgc actgaaagtt cacgttgatg aatacgacgt
     2701 tgatgtgatc gacagccaga gcgccagcaa actgatccca gcagcagttg aaggtggtct
     2761 gcatcagatt gaaacagctt ctggcgcggt actgaaagca cgcagcatta tcgtggcgac
     2821 cggtgcaaaa tggcgcaaca tgaacgttcc gggcgaagat cagtatcgca ccaaaggcgt
     2881 gacctactgc ccgcactgcg acggcccgct gtttaaaggt aaacgcgtag cggttatcgg
     2941 cggcggtaac tccggcgtgg aagcggcaat tgacctggcg ggtatcgttg agcacgtaac
     3001 gctgctggaa tttgcgccag aaatgaaagc cgaccaggtt ctgcaggaca aactgcgcag
     3061 cctgaaaaac gtcgacatta ttctgaatgc gcaaaccacg gaagtgaaag gcgacggcag
     3121 caaagtcgtt ggtctggaat atcgagatcg tgtcagcggc gatattcaca acatcgaact
     3181 ggccggtatt ttcgtgcaga ttggtctgct gccgaacacc aactggctcg aaggcgcagt
     3241 cgaacgtaac cgcatgggtg agattatcat tgatgcgaaa tgcgaaacca acgtcaaagg
     3301 cgtattcgca gcgggtgact gtacgacggt tccgtacaag cagatcatca tcgccactgg
     3361 cgaaggtgcc aaagcctctt tgagtgcttt tgactacctg attcgcacca aaactgcata
     3421 agaagaagta agattcacct gcaattgctt agccgccggg gtcaaacctg gcggcttttt
     3481 tatggcatta aaaagccctg ccgggatgtt ccaggcaggg cggcataaat acttttatct
     3541 gttagcgaat gaccagtaca ggcagattgg cgtggcggat cacgctcgag gcgttagaac
     3601 ctaacagatg ggtcgaaatc gatgggttgc gagaaccgat aactacaaca tcagccccca
     3661 gttcttctgc caactcattg acttcatccc gcacgctacc aaaacggaca tgttgtttaa
     3721 tgcgggaagg atcgatggtg aagtggctga ccatcgtttg cagacgttct tctgcttcat
     3781 gttgcagatg ctcttcaaaa cgacgcacat cagcggcaaa acggtgcagg ctcaggctgg
     3841 ctgacccggg tagtacgtga agtagatgaa taactccgtc atcctgggcg aggaattcag
     3901 cgtggcgaac agctttgtcg ctcaattcca tttcaaatac atcaactggc ataatgattg
     3961 tcttatacat aaccctttct ccctgttaat catgaacaaa tcattcgcca tgattataat
     4021 atttatccct gatatttgtc tggttctttt tccttacgaa cggtttctgt gattaatata
     4081 ttctcactga acaccaggaa ttctcccaaa acctgcggta ccgcccgttt tcccgctgtg
     4141 atagctaccc ttaaagactg actctttttt gaactgtctc tggaggttgc atgaaagcat
     4201 tgacttatca cggcccacat cacgttcagg tagaaaatgt tcccgatccg ggcattgaac
     4261 aggctgatga tattattctg cgtattacgg caacggcgat ctgtggctct gacctccatc
     4321 tttatcgagg caaaataccc taggttaaac atggcgatat tttgggtcat gaatttatgg
     4381 gggaagtcgt tgaaaccgga aaggacgtaa aaaatttgca aaaaggcgac cgggtggtaa
     4441 ttccgtttgt cattgcttgt ggcgactgtt ttttctgtcg aatgcagcaa tatgccgcct
     4501 gcgaaaatac caatgcgggt aaaggcgctg cgctcaataa aaaacagata ccagctcccg
     4561 cggcattgtt tggttatagt cacctgtatg gtggcgttcc tggtggacag gcggaatatg
     4621 tccgcgtccc taaagggaat gtggggccgt ttaaagtacc gcctttgctt tcagatgata
     4681 aagccctttt cctttctgat attctgccaa cggcatggca ggcagcaaaa aatgcgcaga
     4741 tccaacaagg ttcaagcgtt gcagtctatg gtgccggtcc agtgggattg ttgacaatcg
     4801 cctgtgcacg gttgctcggt gcggaacaga tttttgttgt tgatcatcat ccctaccgct
     4861 tgcgtttcgc cgctgaccgc tacggcgcga tcccgattaa tttcgatgaa gatagcgatc
     4921 cggcacagtc aattattgaa caaacggcag gtcaccgggg cgtggatgca gtaatagacg
     4981 ccgtcggttt tgaagcgaaa ggcagcacca cggaaacggt gctgactaac ctgaaactgg
     5041 agggcagcag cggtaaagcg ttgcgccagt gtattgcggc ggtcaggcgt ggcggcattg
     5101 ttagcgtacc gggcgtctac gctggattta ttcacggttt cctgtttggc gacgcctttg
     5161 ataaagggtt gacgtttaaa atgggacaga cccacgttca cgcatggctg ggtgaattat
     5221 taccgttaat tgagaaagga ttactgaaac cagaagaaat tgttacccac tatatgccgt
     5281 ttgaagaggc cgcccgggga tatgagattt tcgaaaaacg tgaagaggag tgccgtaagg
     5341 tgattctggt acccggtgca caaagcgcag aggcggcgca gaaggcggtt tcaggtctgg
     5401 tgaatgcgat gccgggggga acaatatgat cgtcaggagt ggttttcgag gtaaaggaca
     5461 gccatgacga taatcgccgc cataatcaga aatcctatca ggatgtaaaa tgcttctgcc
     5521 atggttattc ccacaaacga aacggaataa ttttgcagca aaagtgaaca gtgagaacca
     5581 ggaaaaattg ctgattttgc gtaaagagga tgcgagtgca tcctctgggc aaagcgagtt
     5641 atcgcttgtg cagatgggat taaagcaggt agtcgccagc agcttctggc tggtattcga
     5701 gttccagcac ttcaaggtgg gttgcaacgc cgcccggaag ttcccagtga atggaatcgc
     5761 caacgcgcag ccccagcagt gcggcaccta ccggagccat aacggaaagc tgagtattgc
     5821 tatcggtcat ttttgccgga tacaccagcg tgcgcacacg cacttcgcca tcgctaagat
     5881 tgcggaattt aacccggctg ttcattgtca ccacgtcgtg tggcatctct tctggcgaac
     5941 acatttgggc gcgatccaac tctgcgttta acgcgtcggc gattggcaaa ccagcatagg
     6001 cgggttgctc cagcagaata tcgatgcgtt cggcatccag gtcgttaatg atgatagttg
     6061 gtctggacat ttttactcca tgtcgtcggt actgcgagtg tcgcagataa acatacccaa
     6121 aagaaaaccc tcaccgtcag gcggcgaggg tttaactcac atgatgatac tgactgttgc
     6181 tcactctttg aagtgatttg cgtcacattc agggaattcc tcaatgcagc gcattatgta
     6241 taattcttaa tcgtcacggt ttatggaaga cgaattagcg tgttttgtaa atcagatgat
     6301 taataaccca ctttatcaat cacaaagttt ttgccacagt tgcctgggtg tggttgtggc
     6361 aagaatgagt ttgcagaaag cggagcgttg atggcgtcag ctttgatcga gatctgaatt
     6421 tcagtcaaat acgcagggtt accctggcac gttagcttaa ctgctttcac gttctctttt
     6481 ccccagcttt tggcaaaggc ggcgtcaaag tcacggcggc tcactgtttt accgtaatta
     6541 tccgcaagga attttccggc ttcgctctct ttaatttctt gattcaggcg taccatcgta
     6601 ccgaagtatg cgtccggatc aaaaccaaag caggcaccgt gtttggcata ttcgtagcgt
     6661 tccaggcagg aacgtccgcc agctcctggc atgacttcac ttagtttagc ggccgtttcc
     6721 agtgataacc cggtttccgg cgatgaacac atccggctgg cgtgcgcttc tggtagattc
     6781 gggattgggc gagtagcgca accgaagcgc atccagcggc gttcatcaac accacgggca
     6841 gcaaccgatt taggcaatcc tggccacaga ccatgtacgg tcagaaaatc agctttgttg
     6901 gtcgtttcgg tttgtaggcg acattcatct cgttcgttac gatttcgatc gtgttgactc
     6961 tggcaaaatc cggtttgcca ggagagggcc aggacatagc gatcaaaatc gccatactgt
     7021 tttgcctgca acgctacggc gttggcagaa gagaagggaa gcagagaaac cgcgagcaac
     7081 gcggcgttac gccagaatgc tttcataatg ggtgtggaac tcatacatac actgaatact
     7141 atctattaaa tcataaaaaa gcccgccagg tgggcttacc tggcgggcgt gatgatttat
     7201 tcagcgtttg gcgaacgtat tagttccaca tggcgagaat cggccagcca accaacagca
     7261 acatagagat gtaaatcacc ccgaagattg cgccaagacg ccagtaatct tttgatttca
     7321 catagccaca gccgtaaata atcaccccag gaccggttgc atacggcgtc agacagccca
     7381 tgataccgat agacagcacc agcaggatac acagttgttc cattggtacg cccggaatac
     7441 ctttaccgac ggccagaata accgacagca tggttgcggt gtgcgcagac aggctggcaa
     7501 acaggtagtg tgcaaagtag aacaccagaa ccagtacaat caccgttgcg tttggtgaga
     7561 atccttccag gtgcgtactc atggtaccgg cgaaccagtc aataaagcca gaacgagtca
     7621 ggccgttagc catcacaacc agagttgcca ggttgaccag cgtgttccat gcgctgttat
     7681 agcgggtaat atctttccaa ggcacaacgt gcagggccag cattagcgaa actgccagca
     7741 gaccaactgc agtagcatta atgacttcac tgccaaaaac ccacaaacct aagctgagca
     7801 atacaaggcc aatcagtgtc cactctctgc gtgtcagcgc acccatggtt ttcagttcat
     7861 cacccgccca ggttgccact tcttcactgt gtgtgatttc cggtttgtac agcacgtagg
     7921 aaagccacgg cgcaatgata agcaagataa ccccaaccgg caggaagcag aggaaccact
     7981 gcaaccagct aatctggata ccggcaattt tgctgacgaa ctccagaccc agcacgtttg
     8041 gtgccgcacc ggtgacaaac atggacgaac tcagactggt actaatgacc atcatccaca
     8101 tcaaatagcc gccaatacga cgcgcggacg gatcgttggg gaatgattta aacagcggcg
     8161 gcaggttttt aatgaccgga aaaaccgtac ccccggtacg cgcggtgttg gacggtgtaa
     8221 acggtgccag cagaatgtcg ataatgacaa tcgcataacc caacgtcagc gtgcgtttgc
     8281 ccatgaattt caccaggaaa agggcaatgc gacgacctaa cccggaaact tcatacccta
     8341 atgcaaaaat aaatgcgcca aataccagcc ataccgtggt gctggaaaaa ccagccagac
     8401 cccatttcag cgcctgtttt tgcgcattaa acgctgggtc agctaattct ttggcatcaa
     8461 agagcaggta attactgcca ataacgcaaa tagtaaccgc aataaaactg atcgctgttg
     8521 ccggaattgg ctcgaggatc atgccgacaa tcattgccac aaacacagcg aagtaatgcc
     8581 atgcctgtgg tggcataccg tcggggacag ggataagaaa catgacaccc atcaccacca
     8641 gtggggccaa tagtttccat atattatctt ttgctaaaga catacgggtt ctccgaaaat
     8701 taatatttcc aaatttatca agtgcttaaa taattaaatc tgtgctaaaa accaggtaag
     8761 gatcagcagg tcggcactgc cgcctggact gagatttcgt tcgatacact ccctgtcgaa
     8821 ctgccggaga taatcgagat cggcgggggt tcgaatgccc cctttttgca ataatgtttg
     8881 cgcctcgcgc tgtagccagc gcaggccccc ctcgccaccg cgcgatgcaa cgttggtatc
     8941 gccgttgatc gccatcagta ggagcaaggt atcgagcaat gccagttcag gatctaaccc
     9001 ctgatccagc agagtgaggt aatgcggcaa ggcgtgattg atcaccagtg gataacccgc
     9061 ttcggcttca ccgcgtgcgc cggtaaggcc aagctgctgg tacaaccgtt gacctgccgt
     9121 cagttgtgaa ttattggtac gcagttctcg atcggtcagg ccacggcaga aacttgccgc
     9181 cgtagaacaa acggttgttg gcgttaccgg ttggttgagt tgaagcaaac ggccaattgc
     9241 cgcacatagt agccctaaag aaaaaatgct gcctttatgc gtgtttacgc ccgcagtggc
     9301 gcggaacata tcaccttcgc aagccatacc aattgggcgt aatccgtgga gtaccgcttc
     9361 tggtgctatt tccgcactac aggcaccaaa ttcaatgaaa cggggtagcc agccctgaat
     9421 cgccagcgcg ctgcggtgga aatcttccag cgccatatct ttatgtgcac cgcagttaat
     9481 ccgatccacg aggcctggtt tcggtgacag attgacttca gtcagcatgg cgcgccagcc
     9541 cagcagggcg tactcatcga tcaatgacgt cgcaagcttt gtggttttag ttgacgttgc
     9601 aggcatcgac atcgttcagc agtgcctcca tgcggttgag taaatcggtc agttgatggg
     9661 tttttccacg cgcgcagacg gctgcgcttt gctcgcacaa caggcagcgg cgaggcggca
     9721 gtgaatagtc gcggcgggag agaatttcgc cttcgggcgt caggacatcg atatcccata
     9781 accgcccgag aggatgacta tgttcaagct caatggtggc gagcttgagg tcgcgagccg
     9841 gggcggcaat gctcaacatg ccctccggcc cgctggcgga aaccagtgca gcctgctcct
     9901 gaatttgcca gccctgtttt gcggctaagg cacgcaaggc tgtcacgcca tgattaaaaa
     9961 ttcggcgtgt gacctcgctg tctttaatcg gcccaggcgc aaccacggta aaggagacca
    10021 gtggaacagg atggcgcttg agccagacgt gttgccgtgc ttgcctttca tcccggctga
    10081 cgagcagctc gggaattgac accgcatggt ggctggcgag ttcaggaagc aggtgcatgg
    10141 cttattcctt cacctgatgc acaacatcga tcaccgagcc atcgcggtaa cgcacaacgg
    10201 caacgacgcg gtcggtgaat tcaatcggct gtggttcacc ggtcagcaga cgcgcgcgtt
    10261 cgcgcagcca ctcaatggaa accactttaa tgcccgcttc ctgcagacgt tctgccagtt
    10321 ccggacgtgc cgggttaact gcgataccgt ggtctgtgac cagaatatcg acactggagc
    10381 ctggggtgat gcaggtcagc acgttatcca ccagagtcgg aatacgaccg cgtaccagcg
    10441 gcgcgacgat gatggaaagc gcagaggcaa tcgcggtatc cagtgaccac cggaagcacc
    10501 acgcagtacg ccgtcagagc cggtcagcac gttaacgttg aactgggtgt caatttccag
    10561 cgcgctcagt accaccacgt cgagacgatc aaccgatgcg cctttcgaac cccagttagc
    10621 gtactggttg gcgctgattt cgatgtgatt ggggttacgg gccagcgatt gcgcagcatg
    10681 gctgtcaaag ctctgcacat ccagcagttt gcggatcaga cctttttcgt gcagatcaac
    10741 catcgtcgcg gtaataccgc cgagggcgaa gtcggcgcga atatcgcggc tacgcatttt
    10801 gtcttccagg aaacgggtta ccgccagcga tgcgccgccg gtgccggttt gcatggagaa
    10861 accttctttg aagtagccag agttgacaat cacatccgca gcgctacggg caataagcag
    10921 ttcgcgcggg ttagtggtca tacgggtcgc gccagcgccg atttttgcag catcgccaac
    10981 gcggtcaact ttgacgatca aatcaacctg atcttgctca atgcttgccg gattatgcgg
    11041 ataaggcagc agttcttcgg taagcatcac aacctgtttt gcgttgtcgg catcaaccat
    11101 tgcatagccg agggagccgc agcaggcttt accggtgtaa ccgttggcat taccgaattc
    11161 atcacaggac gggacgccga ggaaagccac gtcgatattc agttcgccgc tctgtaccag
    11221 atgcacacgt ccgccgtgag agtggatttg caccggttcc gcaagcagac cacgggaaat
    11281 ttcttctgcc agtggaccgc gcaggccgga ggtataaatg cgggtaacca cgccctggcg
    11341 aatgtgttct accagtggcg catggcaatc actcagggag ctggacgcca gggtcaggtt
    11401 tttaaagccc atcttagcga tgacgtccat caccatattg acggtcaggt caccgccacg
    11461 gaaagcgtga tggaaggaaa ccgtcatgcc gtcctgtaaa ccagagcgac gaatcgcttc
    11521 ttcctggttg gcgcacagtt tgcgatcgcg cgctttttca gcctggtagg tttgctttgg
    11581 tgagttctgg aaagcggcaa gatcgcattc agcgcgacga ttccaggccg ctacccgttc
    11641 ttgtcgttga gattgttcaa ttttctgcgt cattttgatt gccttattct tcgcggatgc
    11701 cggaaagttc tgcacgggag agcaccagac gggcacgatc gataaccgga ccgtccacca
    11761 tcttgccgtt cagggaaacc acgccgaggc cttcgcgagc ggcggcttca gcggcttcta
    11821 cgacgcggcg ggcgtgatcc acttctttct gggtcggtgc gtagaggttg tgcaacagat
    11881 caatctgacg cgggttgatc agcgatttgc cgtcaaagcc cagctgtttg atgtgggcgg
    11941 cttcttgcag gaatccggct tcgttgttag cgtcggaata gacggtatcg aacgcctgaa
    12001 tacccgcaga gcgcgcggcc tgcaaaatgg aacagcgtgc gaacagcagt tcagttcctt
    12061 ccggggagcg ttctgtacgc aggttgcgca catagtcttc tgcaccgagg gcgataccga
    12121 tcaaacgctc ggaagcgtga gcgatttcca ctgcgcgggt aatccccagc ggagattcaa
    12181 tcgccgccag caggccggtg ctgccgggtt cacgaccaca ggctttttcg atacgcagga
    12241 tctctttttc gatatccaga acatcctgag cggtatcggt tttcggcaga cgcacaacgt
    12301 ccgcaccacc gcgaacgacg gcttccaggt cgttgacacc ccattcggaa tccagcgcgt
    12361 tgacacgcac aatggtttca atatcgcgat acagcggatg ttgcagcgcg tggtaaacca
    12421 tgcggcgggc ggtgtctttt tcacgcaatg ctacggagtc ttcgaggtca aacatcaggg
    12481 catcagccgg gtagatgaag gagttgctga ccatcgcggc attggcacca ggcacaaaca
    12541 acatgctgcg gcgggtgcga gttttacgtt gttgcagcga agcggaaatc attggcaatc
    12601 ctcccatggc agagccggga taccgctggc gcgtgccagc agggcttcca gtcgtgcacg
    12661 taaaatgcag tccagtgcgc ctttgtcatc gacattcagc tgtacgccgc gcacgttgta
    12721 gcgggcgaga acgtccagaa tggtggtgcg aattgcatcg ccaaactgtt tctcaacgct
    12781 gctattgatt tgcaggtcga tatcctgcgt atcgagtggg gcgatgcgta tcatcacatc
    12841 cccagactca agggtgcctg caacggcggg ctggtttatt ttcatttttc acctgtttct
    12901 catgcggggg tcttttgacg agctgccgcg tcctggcggg agtgctcaag caggttctgc
    12961 aaataatgca gcgtgactgc agggaccagc ggcgcgatag ccgtgagatc gtttttcgcc
    13021 agcagttgac gtacccggga agcggatatc ggcatctcct ggtaacgcag ccgctcaatt
    13081 tcaaccagtt cgatgggcgg tgcggagata gtcggcgttt ccagccagta gcgcatatcc
    13141 tggttgtact gggcggtaac gcgacaaaag ggttcagtac cgacaaagcg gtgagttaca
    13201 cccagcgcgg gagcgaggta ctgacggaaa atcttcagat caatttcggt gtaacaatgg
    13261 ttaatgacgc tctgttcttt aatgaagtag caagggaacg tagcgcggga gatgatgtat
    13321 tcggagccgc gatgcacagt caggcgtgga atatcggcgg tgccttttaa caccagatcc
    13381 aaccggtctt cataggggaa gcgtgaagaa tcttctttga ctaaaaacag atgcaaccag
    13441 tcgcactgtg ccgcagcttg ttgaatcaga taacggtgac cattcgtgaa gggattggcg
    13501 ttcatcacaa tgcagccaat cttgttccct ggatgacgaa attttttcag cgattcggca
    13561 tagcgtttca gccgcgtggc gctgttttcc atcagcacca tcacgccggg tacgctggtc
    13621 agcgtggaaa aaccgcactg gcggaacagc gcctcgtatt cggttttggt ataaataaac
    13681 agatgcgtgc tgtgccgctc ataggcgagg tttatcaatt cagtggctaa tgtcagcgcc
    13741 agtccttcac cgcgaacgga ttcactgata gcaacgcatt taataatatt tccggca
//
LOCUS       DAJVRF010000110        13796 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJVRF010000110 DAJVRF010000000
VERSION     DAJVRF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.110"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..147)
                     /locus_tag="OQN13_002950"
     CDS             complement(<1..147)
                     /locus_tag="OQN13_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCS1616245.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKA"
     gene            complement(344..475)
                     /locus_tag="OQN13_002951"
                     /pseudo
     CDS             complement(344..475)
                     /locus_tag="OQN13_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            476..1021
                     /locus_tag="OQN13_002952"
                     /pseudo
     CDS             476..1021
                     /locus_tag="OQN13_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(1063..2064)
                     /gene="gap"
                     /locus_tag="OQN13_002953"
     CDS             complement(1063..2064)
                     /gene="gap"
                     /locus_tag="OQN13_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCS1616246.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            2253..2783
                     /gene="cybB"
                     /locus_tag="OQN13_002954"
     CDS             2253..2783
                     /gene="cybB"
                     /locus_tag="OQN13_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCS1616247.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3028..3201
                     /locus_tag="OQN13_002955"
     CDS             3028..3201
                     /locus_tag="OQN13_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616248.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="OQN13_002956"
     CDS             complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="OQN13_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCS1616249.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            3821..5461
                     /gene="trg"
                     /locus_tag="OQN13_002957"
     CDS             3821..5461
                     /gene="trg"
                     /locus_tag="OQN13_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCS1616250.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(5499..6422)
                     /locus_tag="OQN13_002958"
     CDS             complement(5499..6422)
                     /locus_tag="OQN13_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1616251.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            6639..7982
                     /locus_tag="OQN13_002959"
     CDS             6639..7982
                     /locus_tag="OQN13_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1616252.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8207..9862
                     /gene="opgD"
                     /locus_tag="OQN13_002960"
                     /gene_synonym="mdoD"
     CDS             8207..9862
                     /gene="opgD"
                     /locus_tag="OQN13_002960"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCS1616253.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            10002..10226
                     /locus_tag="OQN13_002961"
     CDS             10002..10226
                     /locus_tag="OQN13_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCS1616254.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            10289..10825
                     /gene="rimL"
                     /locus_tag="OQN13_002962"
     CDS             10289..10825
                     /gene="rimL"
                     /locus_tag="OQN13_002962"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCS1616255.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(10820..11800)
                     /locus_tag="OQN13_002963"
     CDS             complement(10820..11800)
                     /locus_tag="OQN13_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HCS1616256.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            11924..12916
                     /gene="tehA"
                     /locus_tag="OQN13_002964"
     CDS             11924..12916
                     /gene="tehA"
                     /locus_tag="OQN13_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCS1616257.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            12913..13506
                     /gene="tehB"
                     /locus_tag="OQN13_002965"
     CDS             12913..13506
                     /gene="tehB"
                     /locus_tag="OQN13_002965"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCS1616258.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3540 a         3198 c         3483 g         3575 t
ORIGIN      
        1 tgccttacgg gcatcctcgg cctgaccatc gggtatacgg gaaatgacag cctctgtagc
       61 agggtttacc acatcaatcc atgcgtctcc acgccaggta acaaactgtc catcgatata
      121 cataggatgt tgaacgggta ctgacatggg cgactcctgt gatttatatg tttgttttcg
      181 gtgaatatat tgttaattaa catcaatcat cagagagacg gcatcgggaa aggacaattc
      241 tgtgaagggc ttcataaaaa caagtgtaaa aggaataaaa ggcagaaata aaagtcagga
      301 gtcatgcccg tttgcaaaca agtggttcac aaacgggcaa tttttaacgt aacgagcgac
      361 tttccatcgc ctcgatgagc accgcatcat gtttcagcgt ttgccatgca tggatgactt
      421 ctgccggaaa atcaacgtga acgataaaat ctcgcccgcg gggaccgtag ccattgatca
      481 gttcgtcaat cggctgggtg aagcgatgca ggcggttaaa tttggtaacc ccgctgaacg
      541 caacgacatt gcgatggggc cgttgattaa cgccgcggcg ctggaaaggg ttgagcaaaa
      601 agtggcgcgc gcagtagaag aaggggcgag agtggcgttg ggtggcaaag cggtagaggg
      661 gaaaggatat tattatccgc cgacattgct gctggatgtt cgccaggata tgtcgattat
      721 gcatgaggaa acctttggcc cggttctgcc ggtggtcgca tttgacacgc tggaagaggc
      781 tatctcaatg gctaatgaca gtgattacgg cctgacctca tcaatctata cccaaaatct
      841 gaacgtcgcg atgaaagcca ttaaagggct gaagtttggt gaaacttaca tcaaccgtga
      901 aaacttcgaa gctatgcaag gcttccacgc cggatggcgt aaatccggta ttggcggcgc
      961 agatggtaaa catggcctgc atgaatatct gcagacccag gtggtttatt tacagtcgta
     1021 atgagtaaaa gaggcggagg ttttttcctc cgcctgtgcg cgtcagagtt tagcgaattt
     1081 ttcgagggtg cgaataagct gcgtgacgaa gccatattcg ttatcgtacc aggcgaccgt
     1141 tttcaccagt tgtaaatcgc ccacggcggt aatttccgtt tgcgtggcat caaacaccga
     1201 accgaaatgg ctgccaatga tatcggaaga gactatttct tcatcggtat aaccaaatga
     1261 ctcgttattg gtggttgctt gtttaagtgc gttattcacc tcttcggcag tcactttttt
     1321 tccgagaatc gataccagtt cagtgaccga acctgttttc accggcacgc gttgcgcatg
     1381 acctttcagt ttgccgctca gttccgggat caccagacca atggcttttg ccgcccccgt
     1441 agtgtgggga atgatatttt ctgccgctgc gcgtgaagca cgtaaatctt taccacgtgg
     1501 gccatccacc agtgactggg tgccagtata ggcatgaatg gtcgtcatcg tgccgacttc
     1561 tatcccgaaa ctgtcatgca aggctttggc catcggcgca agacagttag tggtgcatga
     1621 cgccacggaa acaatggtgt cgttgccatc cagagtgtcg tcattgacgt tataaacgat
     1681 agttttcatt tcaccggcag gggcggaaat caacaccttc ttcgcaccag catcaagatg
     1741 tgcctgcgat ttctcggcgg aggtataaaa gccagtacat tcgacaatga tttctgcacc
     1801 tttcgctttc cacggaatat ttttagcctc tttttcggcg taaaccgcga tacttttccc
     1861 atcaacgata agtgaatctt ccgtaaaatc aacgctccag gggaatggtc cgtagtttga
     1921 atcatgtttc agcaggtagg cgagaatttt tggggaagtg agatcattaa tagcgacaac
     1981 gtctatgttg cttttgactt caagtaatcg acgcaacacc agtcgaccga tacgaccaaa
     2041 accgttaata ccaactttac tcatggtttt ctcctgtcag gaacattcgg atgaaaattg
     2101 atcctttcca ggcttagacc aggattgtgg gatgggcaat ctccattctt acagtgaaac
     2161 gtaacgtact gaaaacgggt gaacaatatt taatgaaatt ttgagaaaag cccgttatgt
     2221 taacggaaaa ttatgttaaa gcaggaaatg ttatggaaaa taaatactca aggttacaaa
     2281 tcagcattca ctggctggtc tttttgctgg ttatcgcagc gtattgcgca atggagtttc
     2341 gtggtttctt cccacgtagc gatcggccac tcatcaacat gattcatgtt tcctgcggca
     2401 tctcaattct cgtgctgatg gtcgttcgtc ttttgttaag gctgaaatac ccaaccccgc
     2461 cgattatacc taagccaaaa ccgatgatga cgggactggc gcatttggga catttggtga
     2521 tgtatctcct gtttattgcg ctgccagtga ttggtttggt gatgatgtat aaccggggca
     2581 acccgtggtt tgcgtttggt ttgacgatgc cttacgcttc agaggccaat ttcgaacggg
     2641 ttgatagctt aaagtcgtgg catgagacgc tggcgaatct gggatatttt gtcatcgggt
     2701 tgcacgctgc ggctgcactg gcacaccact atttctggaa ggacaacaca cttctacgca
     2761 tgatgccgcg taaacgttcc tgaaggatat ttcaagaaaa tgcctgtact aaaaccgacc
     2821 cgtggtacag gcgaagaata cgggtctaca tcggaagcgc ctattatatt tatttgtatg
     2881 acaaataaaa ccataatcct tgcccatacg tccatctggc ttatttttaa tcaattcacc
     2941 cgatctttga tctcatcaac ggtatcaaaa taaaccagcg tataacgttc aaatcgaaca
     3001 taagattcga taaaccatgg aggttatatg aaaaaactgg cacttatttt gtttatggga
     3061 acgcttgttt ccttttatgc cgatgccggg cgcaaaccct gttctggttc gaaagggggg
     3121 atctcacact gtacggcagg cggcaaattt gtctgtaatg atggttctat tagtgcatcg
     3181 aaaaaaacat gcactaactg aagtgtaaaa ggggtgccat gagaaaatga atctgctagt
     3241 cagatgcgcg gggaaaatcc ccgcgcttgc ccttacctgg acgtgcaggc catgagcgca
     3301 gcaacctcct tatcaccgtc ccggaaccgc agttcgtaga gcgtttgtcg ggtcaggagt
     3361 gtgaatgtca gaatcgtaat gcagataatg agcagacaca ctaccagagg gttgtgcttc
     3421 atagcctttc tccttgccgg atggcgggta agaggctaag atctgaattg ctaggttcat
     3481 tcgttggcct cggttgatag aaatatcggt cggggccttc gtctttctga ttcccggtta
     3541 gcctgaaaac agaaagtctc aggcacccgc aggcatccta tgaggtttcc ttagggacga
     3601 aaataatcac ttcacgaaat tgcgtgctgt tttccagaat ttttcgtcat tcgggttagc
     3661 cagtttagcc attcgttact ctcttcattc caatagcatt aattttctct gcaataattg
     3721 ttgtaaaaat gtgacgcaaa gaggtttttg gtcataagta attaccgtca agtgccgatg
     3781 actttctatc aggagtaaac ctggacgaga gacaacggta atgaatacaa ctccctcaca
     3841 gcgattaggt tttttgcatc acatcaggct ggttccgtta tttgcctgca ttcttggcgg
     3901 tatcttagtt ctattcgcat taagttctgc tctggctggc tatttcctct ggcaggccga
     3961 tcgcgatcag cgtgatgtta ctgcggagat tgagattcgg accgggttag cgaacagttc
     4021 agattttttg cgttcagccc ggatcaatat gattcaggcc ggggctgcga gttgtattgc
     4081 ggaaatggaa gcaatgaagc gaaatattgc gcaagccgaa tcggagatta aacagtcgca
     4141 gcaagggtat cgtgcttatc agaatcggcc ggtgaaaaca cctgctgatg aagccctcga
     4201 cactgaatta aatcaacgct ttcaggctta tatcacgggt atgcaaccta tgttgaaata
     4261 tgccaaaaat ggcatgtttg aagcgattat caatcatgaa agtgagcaga tccgaccgct
     4321 ggataatgct tataccgata ttttgaacaa agccgttaag atacgtagca ccagagccaa
     4381 ccaactggcg gaactggccc atcagcgcac ccgcttgggt gggatgttca tgattggcgc
     4441 gtttgtgctt gccctggtca tgacgctgat aacatttatg gtgctacgtc ggatcgtcat
     4501 tcgtccactg caacatgccg cacaacggat tgaaaaaatc gctagtggcg atctgacgat
     4561 gaaggatgaa ccggcgggtc gtaatgaaat cggtcgctta aatcgtcatt tacagcaaat
     4621 gcagcattca ctggggatga cagtagggac tgttcgacag ggtgcggaag agatttatcg
     4681 tggcaccagc gaaatttcag ctggcaatgc ggacctgtca tctcgcaccg aagaacaagc
     4741 ggcggctatc gaacaaactg ccgccagcat ggagcaactc actgcgacgg tgaaacagaa
     4801 tgcggataac gcgcatcatg ccagcaaact ggcgcaagag gcttctatta aagccagcga
     4861 tggcgggcag acggtttccg gtgtagtaaa aacgatgggc gctatctcca cgagttcgaa
     4921 gaaaatttct gagatcaccg ccgtcatcaa cagtattgct ttccagacga atattctggc
     4981 actgaatgct gccgttgaag ccgcgcgagc gggtgagcaa gggcgtggat ttgccgttgt
     5041 cgccagcgaa gtacggacac tcgcaagtcg cagcgctcag gcggcgaaag agattgaagg
     5101 cttgatcagt gaatcagtca ggttaattga cctggggtcg gatgaggtgg caacggccgg
     5161 gaaaaccatg agcactattg ttgatgccgt cgcgagtgtc acacatatca tgcaggaaat
     5221 cgccgccgcc tcggatgaac aaagtagagg cataacgcag gttagccagg cgatttctga
     5281 aatggataag gtgacgcaac agaatgcttc tctggtagaa gaggcctcag cggcggcggt
     5341 gtcccttgaa gaacaggcgg cacgattaac tgaggcggtg gatgtattcc gtctgcacaa
     5401 acattctgtg tcggcagaac ctcgcggagc gggtgaacca gttagtttcg ctacggtgtg
     5461 aaaatgttca aggagggatc gacagatccc ttcacctttc agaacggcat tgattttcga
     5521 atagcgttaa tcatcaactg gcaaccagaa gagaacgtcg catctacgcg ggtcagtatt
     5581 ccaatcggtt cgcctgcacc atgtcccgga acaggcaggg ccaccagcgt ggcatgacgc
     5641 aggtcgtctt ttacagcgcc agaagggaca aaccacacgt aatcgtattc aaccgtaagt
     5701 tgacgagata gcgaagcaga cagcgtttcg atacaacccg aaggaatttt acagccctgg
     5761 ctctgcacta atgcatctga atgctggcgt ggcgcagtgc cttctggtga tacaacgacc
     5821 ggccattcca gcacccggct tagcgttacg ttctcctgaa gtagcgggtg attagggcgg
     5881 acaaccagct tcagcgattc aagaaacagc agttcgtaat taagcccggt catcagttca
     5941 ggatctgaca tccgaccaat gccgatatcg atttccccgg tttttaaacc cgccagaatc
     6001 atagggttac tcattgtcgc aacttgcaag gtcgtctctt tttgttgctg atgaaactga
     6061 cctataaccg aaggtaatat ccccagtgcc gcagtaggta gtgcaccaac cctgacgaca
     6121 tcattattaa gaccttcttt acgatgaagc gactgtccgg cagtgttgat ggcgtcaaga
     6181 actctgactg catgcgttaa aaattgttcg ccgggtaagg taagttgcgc cccctgacga
     6241 ccacgctcaa acaagcgagc gcccgtcagc tgctccagtt cattcaatgt cttagagagc
     6301 gcaggttgac tcaaattaag ggtttcagcc gcgcgcccca aagttccttg ttgtgcgaca
     6361 gctacgaatg tatgaaggtg gcgcaaacgg atgcgctgac taaacagact atttttttcc
     6421 ataagcgatg ttaaaaacga agcggtgtcg ctgacaagtg aagttgtttg attatgataa
     6481 cttgattgca aaatattatt aacaattaaa gcaattatgt tacagcaaaa tggataatat
     6541 tgatgttttc gcggcgagat cacagtttgt aaattcttcc cgccagagtg aatgcggtta
     6601 cctacactcc agattactga ccactggagg cagacactat ggcgaacagc atcacggcgg
     6661 atgagattcg ggaacagttt tcgcaggcaa tgtcagccat gtaccagcaa gaagttccgc
     6721 aatatggcac gctgctggaa ctggtagctg atgtgaatct ggctgtgctg gaaaacaatc
     6781 ctcaactgca cgaaaaaatg gtaaatgcag acgagctggc gcgactgaat gttgaacgtc
     6841 atggggcgat tcgcgttggg actgcacaag agcttgctac tcttcggcgg atgtttgcca
     6901 ttatggggat gtacccggtg agctattacg atctctcgca ggcaggggtg ccggtacatt
     6961 cgacagcatt tcggcccatt gatgatgctt ctctggcgcg taatcccttc cgcgttttta
     7021 cctccttact ccgccttgag cttatcgaga acgaaatttt gcgccagaaa gcggcggaga
     7081 ttctgcgtca gcgcgatatc ttcaccccac gttgtcgaca actgttagag gaatatgagc
     7141 agcagggcgg ttttaacgaa acacaggcac aggagtttgt gcaggaagcc ctggaaacgt
     7201 ttcgctggca ccagtcagca acggtagatg aagaaaccta tcgcgccttg cacaacgaac
     7261 atcggttgat tgctgatgtg gtctgttttc ctggatgcca tatcaaccac ctgacgccac
     7321 gtacgctgga tattgaccgg gtgcagtcga tgatgcctga atgcggaatt gaacccaaaa
     7381 ttctcatcga ggggccgccg cgccgcgagg taccgatttt actacgccag accagcttta
     7441 aagcactgga agagacggtg ttgtttgcgg ggcagaaaca gggcacgcat accgcgcgct
     7501 ttggtgaaat tgagcagcgt ggcgtggcat taacgccgaa agggcgacaa ctgtatgatg
     7561 atcttctgcg taacgctgga accgggcagg ataatctcac tcaccaaatg catttacagg
     7621 aaaccttccg cacttttcct gacagtgagt ttttaatgcg tcagcaaggg ttggcatggt
     7681 tccggtaccg tctgacgcct tcgggtgagg cgcatcgtca ggcgattcat cctggagacg
     7741 atccacagcc cttaattgaa cgtggttggg tagtggcgca acccatcacc tatgaagatt
     7801 tcttgcccgt tagcgcggcg gggatcttcc agtcaaatct gggtaatgaa acgcagacac
     7861 gcagtcacgg taatgccagt cgcgaagcat ttgagcaggc gttgggttgt ccggttttgg
     7921 atgagttcca gctttaccag gaagcggaag aacgcagtaa acgtcgctgt ggtttgcttt
     7981 aaattctgac catccgcctt tgcaaaaatt tgcctgattt tacaaacgaa tcaggctcat
     8041 cccatcgaca taaaaaaaat gccgatttat gcatattctc tcagttcaac aattggatta
     8101 ttaataaata ttgtctagag tgagcggtca taaataagca ctttcttgcc gctgaaaacg
     8161 accagcgcgg gaccattcac aacaccagaa ggactcactt tcaggtatgg atcgtagacg
     8221 atttattaaa ggttcaatgg ctatggccgc cgtgtgcggt accagcggca ttgcttctct
     8281 tttttctcag gcggcattcg cggcagattc tgatattgcc gacgggcaaa cccagcgttt
     8341 tgacttctcc attctacagt caatggcgca cgacttagcg caaacagcgt ggcgtggtgc
     8401 gcctcgtccg ttacctgaca cgctggcgac aatgacgccg caggcttata acagtattca
     8461 atacgacgcc gaaaaatcgc tctggcataa cgttgagaac cgtcaactgg acgctcagtt
     8521 cttccatatg ggaatgggat tccgtcgccg cgttcgtatg ttttctgtag atccagcaac
     8581 acatctggcg cgtgaaattc actttcgccc ggagttgttc aaatacaacg atgcaggtgt
     8641 tgataccaaa caattagaag ggcaaagcga tctcggcttt gccggttttc gcgtgtttaa
     8701 agcccccgaa ctggcgcgcc gtgatgtagt atcatttctc ggcgcgagtt atttccgcgc
     8761 cgttgatgat acatatcaat acggtttgtc ggcccgcggc ctggcgatcg acacttacac
     8821 cgacagtaaa gaagagttcc ccgactttac cgccttctgg tttgatacgg taaaaccggg
     8881 ggcaactacc tttaccgttt atgcgttgct cgatagcgcc agtattactg gtgcctataa
     8941 gttcactatc cattgtgaga aaagtcaggt gattatggat gtggaaaatc acctgtatgc
     9001 gcgcaaagac attaaacagc tgggcattgc gccgatgacc agtatgttca gctgcggtac
     9061 taatgaacgt cggatgtgcg acaccattca tccgcaaatt catgactctg atcgtctgtc
     9121 catgtggcgg ggcaacggcg agtggatttg ccgtccgctg aacaatccgc aaaaattgca
     9181 gttcaatgct tacaccgaca acaacccgaa agggtttggt ttattgcaac tggatcgtga
     9241 cttctcccat tatcaggaca ttatgggctg gtataacaaa cgcccaagtt tgtgggtgga
     9301 accgcgtaac aagtggggta agggcaccat cggcctgatg gaaatcccaa caacgggcga
     9361 aacgctggat aacattgtct gcttctggca gccagaaaaa gctgtaaaag caggtgatga
     9421 gtttgcattc cagtatcgtc tgtactggag tgcgcaaccg cctgttcatt gcccattagc
     9481 gcgcgttatg gcgacgcgta ccggcatggg cggtttcccg gaaggttggg cgccaggtga
     9541 acactatccc gaaaaatggg cgcgtcgttt tgccgtcgat ttcgttggtg gtgatctgaa
     9601 agctgccgcg ccaaaaggca ttgagccggt gattacgctt tccagtgggg aagcgaagca
     9661 aatcgaaatt ctctatattg aacccatcga tggttatcgt attcagtttg actggtatcc
     9721 gacttcggac tctactgatc cggtcgatat gcggatgtat ctacgttgtc agggggacgc
     9781 tatcagtgaa acatggctgt atcagtattt cccgccagcg ccggataaac gtcagtatgt
     9841 tgacgaccgc gtgatgagtt aatcgttttt tcttcggcac cttcttcggg aggtgccgtc
     9901 tggttaaaca cgatcccgct cgcatttttc cctaagttaa atgagtaatc tgatggtgtg
     9961 tatttcagat acaccttgtc agccactaac agggagtgcg tatgtttcca gaataccgag
    10021 atttaatatc ccgtctgaaa aacgaaaatc ctcgctttat gtccttgttc gataaacaca
    10081 ataaacttga tcatgaaatt gccagaaagg aaggttccga cggtcgaggg tacaatgcgg
    10141 aagtggtccg catgaaaaaa caaaagctac agttaaaaga tgagatgctc aaaatcctgc
    10201 agcaggagag cgtcaaagag gtgtaaactt tcctaagccg ccagccaggc ggctttttta
    10261 acaacagcat ggattgactg gagataagat gactgaaacg ataaaagtaa gcgaatcact
    10321 tgaattacat gctgttgcgg aaagtcacgt cacacctctt tatcagttaa tctgtaaaaa
    10381 taaaacctgg ttacagcagt cgctaaactg gccgcagttc gttcagagtg aagaggacac
    10441 gcgaaaaacg gtgcagggta atgtgatgtt gcatcaacgc ggctatgcca aaatgttcat
    10501 gattttcaaa gaagatgaac ttatcggcgt tatctcgttt aatcgtattg aaccactgaa
    10561 taaaactgct gaaatcggct actggctgga cgaatctcat caggggcagg ggatcatttc
    10621 tcaggcgctg caggcattga ttcatcatta cgctcagtct ggtgaactta gacgcttcgt
    10681 gatcaaatgt cgggtggaca atccgcaaag caaccaggtc gctttgcgca atggttttat
    10741 ccttgaaggt tgcctgaaac aggctgagtt cctgaatgat gcctatgatg atgtgaactt
    10801 atacgcgcgt attatcgatt cataataacc ctgccagcgg cgttcgcgta atgcgctcct
    10861 cgccgttaat gacttttgga ccacggacat gaaccgtgtc gccatcaaag gcttcaacga
    10921 cggcgtggtc ggttaactcc acatggtttt caattatcac cgcacctgaa atacggctct
    10981 caccttgtat tacaacgtgc tcgtcgagca gaatcggtcc accacgtacc acggcatttc
    11041 cgccaatcaa gacatgatgt tttaacacac aattaccttc cacaatggca tattccgcca
    11101 cctgcgaact gtaatgaatc gtcggaatgg catcttcttc tatgccagct ttcacctgcg
    11161 cgtgaccgta gacttttgcg caatcgcata gccagacgtt gttctcttca ttaccttcaa
    11221 cactggcaaa atcaaaaact tcggcgcgat gttcaataaa agcataccgg acgacggcat
    11281 cgccataaat ttgtgcctgg tggacaatac gcgaggcgct aacccttgcg cgatcataaa
    11341 tttggagcag gagttgatgg tcgggcgtta agccttgtgc ggcgacgatc atagaatgtt
    11401 gatcaattaa ggcgtgacca aatattctac attgcccgta aactaatgag tcacgaatgg
    11461 tgacactgtc acttatatat gcaccctggc tgatttcact attatcgatc cagacgttat
    11521 ccgttgcgta aacctctccc cacagcacgc tggtgccggt gatacgcgta ttaccagaaa
    11581 tcaccgttcc gccgaacgca atagcattct ggtcgtaaat ccagcaattt ccttcctgtg
    11641 ctaacaccgt ttcgcgatcg atccagccgc cagcggttcc tgctatcaca tcgttaaaat
    11701 cgctaatggc gataatctgc cgtaataaca cacttttttt agtgccatct tcctgataac
    11761 taaaggctcg ctgttcttca cttagacgat atttgcgcat agcgttttct cacaggtgac
    11821 ttactataac cgtagcaaat tctgcggctc tggctatgct cgagaaaatt ccataaaatg
    11881 catttcaaat atactttata aattaaacaa aatgagtaag aagatgcaga gcgataaagt
    11941 gctcaatttg ccggcaggct actttggtat tgtgttgggg acgataggga tgggatttgc
    12001 ctggcgctat gccagccagg tttggcaggt cagccactgg ttaggggatg ggctggtgat
    12061 tctggcgatg atcatctggg gattattaac tagcgcattt attacccgac tcatacgctt
    12121 tccgcatagc gtgctggcgg aagttcgcca tccagtgctg agcagttttg tgagtttgtt
    12181 tccggcaacg acgatgctgg tggcgattgg ttttgttccg tggtttcgcc cactggcggt
    12241 gtgcctgttc agttttggtg tcgtggttca gttggcttat gccgcctggc aaactgcggg
    12301 attatggcgc ggatctcacc ctgaagaagc taccacgcct ggactgtatc tgccgacagt
    12361 tgccaacaac tttatcagcg caatggcctg tggtgcgttg ggctacaccg acgccggtct
    12421 ggtgttttta ggcgcaggcg ttttctcatg gctaagtctt gaaccggtga tcttgcagcg
    12481 tctgcgcagt tcgggagaat tacccacggc actgcggaca tcactcggca ttcagctcgc
    12541 tcctgcgctg gtggcctgta gtgcctggtt gagcgtcaac ggcggcgagg gtgatacgct
    12601 ggcgaaaatg ctttttggtt atggactgct gcaactgctg tttatgctac gtctgatgcc
    12661 atggtatctc tcccagccat ttaatgcttc attctggagt ttctcgttcg gcgtatctgc
    12721 actggcaacc accggtttgc atctggggag tggcagcgaa aatggatttt tccatacgct
    12781 ggcggtaccg ctgtttatct ttaccaattt tattattgca atactgctca tccgtacttt
    12841 tgcgcttctg atgcagggaa aattgttagt cagaaccgag cgcgccgttt taatgaaagc
    12901 agaggacaaa gaatgatcat tcgtgacgaa aactatttta ctgataaata tgaattaacc
    12961 cgcacacact ctgaagtact ggaagcggtg aaagtggtta aaccgggtaa aacgctggat
    13021 ctgggctgtg gcaatggtcg taacagtctt tacctggcag ccaatggtta tgatgttgac
    13081 gcatgggata aaaatgccat gagtatcgcc aatgtagagc gcattaaatc cattgaaaat
    13141 ctggataatt tacacacccg agtcgttgat ctgaataacc tcacatttga tagacagtac
    13201 gattttattc tttcgactgt ggtactgatg ttccttgagg ctaaaaccat cccagggctg
    13261 attgccaata tgcaacgttg cactaaacct ggtggttaca acctgattgt ggcggcgatg
    13321 gataccgctg attatccatg taccgtcggc ttcccgtttg ccttcaaaga gggagaatta
    13381 cgtcgatatt acgaaggctg ggagagggtg aaatacaatg aagacgtcgg cgagctgcac
    13441 cgcaccgacg ccaacggtaa tcgtattaaa ctgcgtttcg ccacgatgct ggcacgtaaa
    13501 aaatgacccg ctaagaataa ggcgcgtgaa aattccccac gctgagatga tttactgttc
    13561 ttcttttcgg taagcatatt ttttatcgaa gggatgtgaa attaatcaca gtagtcgaag
    13621 ttttttagca gcttaactta ctgaaattta actactgatg attgacttag cccctttttc
    13681 ggcattgact atgtcgtctg aaaaggggct gaaaaattta ttttcaccaa cacttttttt
    13741 gccacaacac gaagcggcgc tttttgctat aacttaaaaa gtaatataat catctc
//
LOCUS       DAJVRF010000111        13753 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJVRF010000111 DAJVRF010000000
VERSION     DAJVRF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13753
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.111"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            19..1245
                     /gene="rtcB"
                     /locus_tag="OQN13_002966"
     CDS             19..1245
                     /gene="rtcB"
                     /locus_tag="OQN13_002966"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCS1616259.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            1249..2265
                     /gene="rtcA"
                     /locus_tag="OQN13_002967"
     CDS             1249..2265
                     /gene="rtcA"
                     /locus_tag="OQN13_002967"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCS1616260.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2308..5013)
                     /gene="malT"
                     /locus_tag="OQN13_002968"
     CDS             complement(2308..5013)
                     /gene="malT"
                     /locus_tag="OQN13_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCS1616261.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIHTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5624..8017
                     /gene="malP"
                     /locus_tag="OQN13_002969"
     CDS             5624..8017
                     /gene="malP"
                     /locus_tag="OQN13_002969"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCS1616262.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8027..10111
                     /gene="malQ"
                     /locus_tag="OQN13_002970"
     CDS             8027..10111
                     /gene="malQ"
                     /locus_tag="OQN13_002970"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCS1616263.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="OQN13_002971"
     CDS             complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="OQN13_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS1616264.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKRIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="OQN13_002972"
     CDS             complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="OQN13_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCS1616265.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="OQN13_002973"
     CDS             complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="OQN13_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCS1616266.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13188..>13753
                     /gene="bioH"
                     /locus_tag="OQN13_002974"
     CDS             13188..>13753
                     /gene="bioH"
                     /locus_tag="OQN13_002974"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCS1616267.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLR"
BASE COUNT         3323 a         3422 c         3863 g         3145 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgctggga acgggtaacc actttattga aatctgcctt
      541 gatgagtcgg agcaggtgtg gattatgctg cactccggtt cacgcggaat tggtaacgcc
      601 atcgggactt actttatcga tctggcgcaa aaagagatgc aggaccaact tgagacgttg
      661 ccgtcgcgtg atctggcgta ctttatggaa ggtacggaat actttgatga ttacctaaga
      721 gccgtggcct gggcgcagct tttcgccagc cttaaccgcg atgcgatgat ggaaaacgtg
      781 gtaacggcat tgcagagcat tacgcagaaa acggtcagac agccacaaac gctggcgatg
      841 gaagagatca actgtcacca caactatgtg caaaaagaac agcactttgg tgaagagatc
      901 tacgtgacgc gtaaaggcgc ggtgtttgcg cgtgctggtc aatatggaat tattccaggg
      961 tcgatgggag caaaaagctt tatcgtccgt gggctgggaa atgaagaatc tttctgttcg
     1021 tgcagccacg gtgccgggcg ggtaatgagc cgtactaaag cgaaaaaact gttcagcgtg
     1081 gaagatcaaa ttcgtgccac cgcgcatgtg gaatgccgta aagatgccga agtgatcgac
     1141 gaaatcccga tggcgtataa agatattgat gcggtgatgg cggcacaaag cgatctggtg
     1201 gaagttatct ataccctgcg tcaggtggtg tgcgtaaaag gataaatgat gaaaaggatg
     1261 attgcgctgg atggcgcaca gggcgaaggt ggcgggcaga tcctgcgctc ggcgctgagc
     1321 ctgtcgatga taaccggcca gccatttacc atcaccagca ttcgtgccgg gcgggcgaaa
     1381 ccggggctgt tgcgccagca tctgaccgcg gtaaaagcgg ctgcggaaat ttgtagggca
     1441 acggtggaag gcgcggagct ggggtcgcag cgtctggtct tccggcttgg caccgtgcgc
     1501 ggcggcgagt accgctttgc tatcggtagt gccggaagct gtacgctggt gctgcaaacg
     1561 gtgctgcccg cgctgtggtt tgccgatgga ccttcgcgtg ttgaagtgag cggaggcacc
     1621 gataacccgt cggccccgcc tgcggatttt atccgccggg tgctggagcc gctgctggcg
     1681 aaaataggaa ttcatcagca aaccacgctg ttacgtcacg gtttttatcc tgccggaggc
     1741 ggcgtggtgg caacggaagt ctcgcccgtg gcatcgttta acaccttgca acttggcgag
     1801 cgcgggaaca ttgtgcagat gcgtggagaa gttctattag ctggtgtgcc gcgccatgtt
     1861 gctgagcgtg aaatcgctac actggcgggg agtttttccc tgcatgaaca gaatattcat
     1921 aacctgccgc gcgaccaggg gccgggtaat accgtctcgc tggaagtcga aagtgaaaat
     1981 atcactgaac gctttttcgt cgtcggtgaa aagcgcgtca gtgccgaggt ggtagccgcg
     2041 cagttagtga aagaggtgaa acgctacctg gcaagcccgg cggtagtggg ggaatatctc
     2101 gccgaccagc tggtgctacc gatggcgctg gcgggcgcgg gagaatttac ggtcgcccat
     2161 ccttcatgcc atctgctgac caatatcgcc gtggtggagc gtttcttgcc ggtgcggttt
     2221 ggtttgatag aaacagatgg cgtaacgcgg gtgagtattg aatgatgtaa agatgtaaac
     2281 cggatccaat gcattatccg gctaaactca caccccatat cccatcatct tcagcagttt
     2341 ctgcgcgtgt tgtaccgcgg cctgacggtg ggcgacgccg agtttttggt acagattgcg
     2401 gatatgcgtc ttgatggtgg ttgccgccac ttccagctcg cccgcaattt gctcattgct
     2461 gtaaccggag tagatcagcc ccaggacctg ccattcacgt tgggtcaacg ggctggtgtg
     2521 gatcagttct ggcacttccg gatgattcag cagacgttca acgaaattct catcgaaatg
     2581 ggcgaattta tgccgatgat gttgattgat ttctcgcaga atacgctgcg cgcgatgctg
     2641 ttccagttcc ggcagcgtat tgagttgaat cagctggcgc agttgttgcg ccatcgcttc
     2701 gccttcgatg acaaaatgac tgataaatcc ggtgcgattc gccagtttta atgcgtccag
     2761 caacacgcgc tgggcgtcac ttttacgtcc ggcctgccag tacagttgat taagcagcaa
     2821 caggttacgg ttgagatcgc tcatcaaccg cagactgcgg gcattttcat tgagttcttc
     2881 gagaacaatt tccgcaggtt caaactcgcc cagcaagatt tgtgcgcggg caatgttgcg
     2941 ccattgacct tgcaggaagt ggttgttcgc aaactccggt ttagccgtat gacgcaacca
     3001 gttggcagcg gcggctttat cgccggtcat ttgccagtaa atcacccgga ctttgttggc
     3061 gttagagatc cagtcgctgt gatatttgcc attccccagc aggttttcca gacggttcag
     3121 ctggctacgg gcgttatcta aatcaccacg ggccagcgag cattgaatca acattgccag
     3181 gcactgaagc tgttgctgtg gctgataaga cgacaagact tcaatcccgc tacgcgccga
     3241 cgcttcggct tcatccagcc gtgcccaggc ccataacagt tgtgctcgga tgcgcaccag
     3301 aaactcatgc atcggcagct gttccagatg ctgctcgttg atcagctgga acgttttttc
     3361 ctgtgtttcc cacgcggttt gcaggaaccc ttgggcaaac agaatttcac tttgctggat
     3421 taaactccat aaggcgtagt gccagacgtc gtgctggcgt gccatctgtt cggtttgctg
     3481 cattagcgcc agtgagcggg tcaattcgcc tttgcagtgc agcacttcac ccagcaccga
     3541 ggttgctaca atgcggctat agaaccagcc cggtggcagc tcttccagtg ccagttttgc
     3601 cagccgttcc gcttcatccg gattaccatc gttaatcgcc acctgggcgc gcagagcgtt
     3661 aaattctgcg tgcatggtgc cttctctgat gtccttgatt tcatgttcag cacgggctag
     3721 cagggtgtta acttcgccat agcgatgttg gctttgcatt agccacgcct gcaataacac
     3781 taactgcgga ttttccagca aactgtccca cggcagggcc ttaagcgact cttccagcag
     3841 cgacagttcg ctatggttga acaaactcca ggcgtgatta agcagaatat cgcgcagcat
     3901 cagcgcatcg cctgccgcca gcgcatgatg aatcgcttcg ctgggaaatc cctgggccat
     3961 ccagctttct gcggcggcac ggtggatttc cggcagctcc gccgccagtt cccactggca
     4021 gcgctggcgc aggaagttgc caaacagcgg gtgatagcag aaccactcgc cggtatcatc
     4081 catccgctgt aaaaacagcc cctgacgctc aatctcttcg agacgcattt gcccgttttc
     4141 ttcgccagtc acacgggtga tgagggcatc gttcattgag cgcaaaatgg cgcttttcaa
     4201 cagaaaatgg cgcgttgcga gatcgacgtt atccaaaacc tcatcgacca gataatccga
     4261 aagatggctg gcattgattc ccgccaggcg gcgtgccgac ttatgggctg agtgggtatt
     4321 ctgccgggcg gagagggcga ttagctgtag tgccgtcgcc caaccggaaa cgtcatcgca
     4381 aatccaactg ctttctgcgg cttcaatcgg cgatgacaga cggcaatcaa aaaactgctt
     4441 cgcttcctga tgggtaaatg ccagttgctg actgccaatt tccagcagtt ggtcacgaac
     4501 acgcagattg gcaatgccca gttgcggaag gttgcgtgac aacaccacca gggtgagatt
     4561 ttctggttga tggcgaataa agaagcgcat tgactcgtgg atcactggat tagtgatcag
     4621 atgatagtca tcgatgacca gataaagtgg gctatgccat tccgccagct caatgaaaag
     4681 ctgggcgaag agtgacgtca ggctggcata ttgccgtttt tgcgccatcg tctcacatat
     4741 cgcacagtga ccgttggttg cctgctgcac ggcggcaatg agatagctgg cgaaacgctc
     4801 ttgctggtta tcaccttcat ccagcgagta ccagccgata tcgtttttgc ctgccgccca
     4861 ctgggaaatg agggtggtct ttccgtagcc cgcaggactc gtgatcagcg ccagccggaa
     4921 gttgttcgcg ccggaaagtt tagccagcag gcgctcacga accacggtat ggtcgagtcg
     4981 aaccggacga cttagttttg acggaatcag catagttaat cacttcactg tggaaaatga
     5041 ggaaatatta tttttttgcg cttcgtaatt aatggttata aggtcggcca gaaacctttc
     5101 taatgcaagc gatgacgttt ttttatgtgt ctgaatttgc actgtgtcac aattccaaat
     5161 ctttattaac aactcaccta aaacgacgct gatccagcgt gaatactggt ttcccttatg
     5221 ttcatcagat tcatttaagc aagggtttct tcttcattcc tgatgaaagt gccatctaaa
     5281 aagatgatct taataaatct attaagaatg agatggagca cattggatat tttacttgtg
     5341 aaactgtttc actcctttac ttaatttata gagttacctt ccgctttttg aaaatacgca
     5401 acggccattt tttgcactta gatacagatt ttctgcgctg tattgcattg atttgctgct
     5461 aatcctgtgg tttgcactag ctttaagtgg ttgagatcac atttccttgc tcatccccgc
     5521 aactcctccc tgcctaatcc cccgcaggat gaggaaggtc aacatcgagc ctggcaaact
     5581 agcgataacg ttgtgttgaa aatctaagaa aagtggaact cctatgtcac aacctatttt
     5641 taacgataag caatttcagg aagcgctttc acgtcagtgg cagcgttatg gcttaaattc
     5701 tgcggctgaa atgactcctc gccagtggtg gctagcagtg agtgaagcac tggccgaaat
     5761 gctgcgtgct cagccattcg ccaagccggt ggcgaatcag cgacatgtta actacatctc
     5821 aatggagttt ttgattggtc gcctgacggg caacaacctg ttgaatctcg gctggtatca
     5881 ggatgtacag gattcgttga aggcttatga catcaatctg acggacctgc tggaagaaga
     5941 gatcgacccg gcgctgggta acggtggtct gggacgtctg gcggcgtgct tcctcgactc
     6001 aatggcaact gtcggtcagt ctgcgacggg ttacggtctg aactatcaat atggtttgtt
     6061 ccgccagtct tttgtcgatg gcaaacaggt tgaagcgccg gatgactggc atcgcagtaa
     6121 ctacccgtgg ttccgccaca acgaagcact ggatgtgcag gtagggattg gcggtaaagt
     6181 gacgaaagac ggacgctggg agccggagtt taccattacc ggtcaagcgt gggatctccc
     6241 cgttgtcggc tatcgtaatg gcgtggcgca gccgctgcgt ctgtggcagg cgacgcacgc
     6301 gcatccgttt gatctgacta aatttaacga cggtgatttc ctgcgtgccg aacagcaggg
     6361 catcaatgcg gaaaaactga ccaaagttct ctatccaaac gacaaccata ctgccggtaa
     6421 aaagctgcgc ttgatgcagc agtacttcca gtgtgcctgt tcggtagcgg atattttgcg
     6481 tcgccatcat ctggcggggc gtaaactgca cgaactggcg gattacgaag ttattcagct
     6541 gaacgatacc cacccaacta tcgcgattcc agaactgctg cgcgtgctga tcgatgagca
     6601 ccagatgagc tgggatgacg cttgggccat taccagcaaa actttcgctt acaccaacca
     6661 taccctgatg ccagaagcgc tggaacgctg ggatgtgaaa ctggtgaaag gcttactgcc
     6721 gcgccatatg cagattatta acgaaattaa tactcgcttt aaaacgctgg tggagaaaac
     6781 ctggccgggc gatgaaaaag tgtgggccaa actggcggtg gtgcacgata aacaagtgca
     6841 tatggcgaac ctgtgtgtgg ttggcggttt cgcggtgaac ggtgttgcgg cgctgcactc
     6901 ggatctggtg gtgaaagatc tgttcccgga atatcaccag ctatggccga acaaattcca
     6961 taacgtcacc aacggtatta ccccacgtcg ctggatcaaa cagtgcaacc ctgcactggc
     7021 ggctctgttg gataaatcac tgcaaaaaga gtgggctaac gatctcgatc agctgatcaa
     7081 tctggaaaaa ttcgctgatg atgcgaaatt ccgtcagcaa tatcgcgaga tcaagcaggc
     7141 gaataaagtc cgtctggcgg agtttgtgaa agttcgtacc ggtattgaga tcaatccaca
     7201 ggcgattttc gatattcaga tcaaacgtct gcatgagtac aaacgccagc acctgaatct
     7261 gctgcatatt ctggcgttgt acaaagaaat tcgtgaaaac ccgcaggctg atcgcgtacc
     7321 gcgcgtcttc ctcttcggcg cgaaagcggc accgggctac tacctggcga agaatattat
     7381 ctttgcgatc aacaaagtgg ctgacgtgat caacaacgat ccactggttg gcgataagtt
     7441 gaaggtggtg ttcctgccgg attattgcgt ttcggcggcg gaaaaactga tcccggcggc
     7501 ggatatctcc gaacaaattt cgactgcagg taaagaagct tccggtaccg gcaatatgaa
     7561 actggcgctc aatggtgcgc ttactgtcgg tacgctggat ggtgcgaacg ttgaaatcgc
     7621 cgagaaagtc ggtgaagaaa atatctttat ttttggtcat accgtggaac aagtgaaggc
     7681 aattctggcc aaaggctacg acccggtgaa atggcggaag aaagataagg tgctggacgc
     7741 agtattgaaa gagctggaaa gcggtaaata cagcgacggc gataagcatg ccttcgacca
     7801 gatgctgcac agtatcggca aacaaggcgg cgatccgtat ctggtgatgg cggatttcgc
     7861 agcctatgtt gaggcacaaa agcaggtgga tgtgctgtac cgcgaccagg aggcctggac
     7921 tcgcgcggcg atcctcaata ccgcccgctg cggtatgttt agctcggatc gctctattcg
     7981 cgattatcag gctcgtatct ggcaggcaaa acgctaagga agctcgatgg aaagcaaacg
     8041 tctggataat gccgcgctgg cggcggggat tagccccaat tacatcaatg cccacggtaa
     8101 accgcagtcg attagcgccg aaaccaaacg gcgtttgctt gacgcgatgc atcaacgtac
     8161 cgccacgaaa gtggcggtaa cgccagtccc gaatgtcatg gtttatacca gcggcaaaaa
     8221 aatgccgatg gtggtggagg gcagcggcga atatagctgg ctgctgacca ccgaagaagg
     8281 aacacagtac aaaggccatg taacgggggg caaagcgttc aatctaccga cgaagctgcc
     8341 ggaaggttat cacacgctga cactcaccca ggacgaccag cgcgcgcatt gccgggtgat
     8401 tgtcgccccg aaacgctgtt acgaaccgca ggcgttgctg aataaacaaa agctgtgggg
     8461 tgcctgcgtt cagctttata cgctgcgatc ggaaaaaaac tggggtattg gggattttgg
     8521 cgatctcaaa gcgatgctgg tggatgtggc aaaacgtggc gggtcgttca tcggcctgaa
     8581 cccgattcat gcgctctatc cggcaaatcc ggagagtgcc agcccataca gcccgtcttc
     8641 tcgccgttgg ctgaatgtga tttatatcga cgttaaagcc gtcgaagatt tccatcttag
     8701 cgaagaggct caggcctggt ggcagttgcc gaccacgcaa cagacgctgc aacaagcgcg
     8761 ggatgccgac tgggtcgatt actccacggt taccgcccta aaaatgacag cattacgaat
     8821 ggcgtggaaa ggtttcgcgc aacgtgatga tgagcagatg gccgcgtttc gccagtttgt
     8881 tgcagatcaa ggcgacagcc tgttctggca ggcagccttt gatgcgctac atgcccagca
     8941 agtgaaagag gacgaaatgc gctggggctg gcctgcatgg ccagagatgt atcagaacgt
     9001 ggattcacca gaagtgcgtc agttctgcga agaacatcgt aatgacgtcg atttttatct
     9061 ctggttgcag tggctggctt acagccagtt tgccgcctgc tgggagataa gccagggcta
     9121 tgaaatgccg attggcttgt atcgtgatct ggcggttggc gtagcggaag gtggggcgga
     9181 aacctggtgt gaccgtgaac tatattgcct gaaagcgtcg gttggcgcgc cgccggatat
     9241 cctcggcccg ttggggcaga actggggatt accgccaatg gacccgcata tcatcaccgc
     9301 gcgtgcctat gaaccgttta tcgagctgtt gcgtgccaat atgcaaaact gcggcgcatt
     9361 acgaattgac catgtgatgt cgatgctgcg tttgtggtgg ataccgtatg gcgagacggc
     9421 agatcagggc gcgtatgttc actatccggt ggatgatctg ctctcgattc tggcactcga
     9481 aagtaaacgt catcgctgta tggtgattgg tgaagatctc ggtaccgtac cggtagagat
     9541 tgtcggtaag ctgcgcagca gcggtgtgta ctcctacaaa gtgctctatt tcgaaaacga
     9601 ccacgagaag acgttccgtg caccgaaagc gtatccggag cagtcgatgg cggttgcggc
     9661 gacacatgac ctgcctacgc tacgcggtta ctgggagagc ggggatctaa cgctgggcaa
     9721 aaccctgggg ctgtatccgg atgaagtggt actgcgcggt ctgtatcagg atcgcgaact
     9781 ggcgaagcaa gggctgctgg atgcactgca taaatatggt tgtctgccaa aacgtgccgg
     9841 gcataaggca tcgttgatgt cgatgacgcc gacgctgaac cgtggtttgc agcgctacat
     9901 tgccgacagt aacagtgctc tgttaggact acagccggaa gactggctgg atatggccga
     9961 accggtgaat attcctggca ccagttacca gtataaaaac tggcgacgca agctttccgc
    10021 aacgcttgag tcgatgtttg ccgatgatgg cgtgaacaag ttgctgaagg atttggacag
    10081 acggcgcaga gctgcagcga agaagaagta gagtgcggtt gatgccggat gcgatgcatc
    10141 cggcagtcag tgttatcaaa tcaccatatt cagcagcaga cagcctacca gaccgcacac
    10201 cgagataatg gtttccagca tcgaccagga tttgatggtc tcgccgatag tcaggttaaa
    10261 gtactctttg aacagccaga agcccggatc gttcacatga gagaaaatca cactaccgga
    10321 acccaccgca ataaccatca gctctgggct aacacccgtc gttgcaatca gcggtgccgc
    10381 gataccaccc gcagtgattg ccgcaacggt tgcggaaccc agcgcgatac gcagtacggc
    10441 agcaatcgac caggccatca gcagcggaga aatgttggtt tcgtgcatca tggaagcaat
    10501 gtatttgtcc acgccgctgt ctaccagcac ctgcttgaac gcaccgccac caccgatgat
    10561 caacagcatc atcgcaatga ttttgatgga agaaaccagc gtgtcgttaa tctgatccat
    10621 tgaacgacca cggttcagac caaaggtgaa catcgcaatc agcacggcaa tcagcgttgc
    10681 cattaccggg tcaccgagga actccgctac cggcaggaaa gcgtgacctt tcggcaggat
    10741 catttcggca atcgcacgca tcgccatcag cacaaccggc accagagaag tccagacgct
    10801 gacgccaaag ctcggcatct cttcttcgct gaaggttttc gcgctgtaga gaccttccgg
    10861 gattggctta tcgatacctt tcaacacgcg agcgtaaacc ggaccggcga gaatcacggt
    10921 cgggattgcc agaatagtac cgtacagcag ggttttaccc atatcggcat tgaaaatggt
    10981 ggcaatcgca gtcgggcccg gatgcggtgg caggaagccg tgggtcacag acagtgcagc
    11041 cgccattggt acaccaacat acagcagtgg aatattcgcg gaagccgcga tggtaaacac
    11101 cagcggcagc atcagcacga agcccacttc atagaacagg gcaaaaccaa cggtaaaacc
    11161 ggttaacacc accgcccact ggatgcgctt tttaccaaat ttggcaatca gcgtggtggc
    11221 gatacgttgt gcgccaccgc agtctgccag cattttgccc agcattgcgc caaaacccat
    11281 gatcagggca aggctaccga gcgtcccgcc gacaccgact ttgatggagt caataacttt
    11341 atccagcggc attccttgca ttaatccaac agcaagcgcc acgaggacga gagcgatgaa
    11401 gccgttcatt ttgaagcgga tcatcaggag caacaacaag attacaccga tagcaacaat
    11461 gactaatggc atgatttacc tggcctttca tttgttatgg gtaacgtcaa ttttctgacg
    11521 acaaactcta attatcccaa tcgggaacag agatattgcg gcaccacgac tgatacccac
    11581 taaaactaat tattgtagtc agatgtcagg agtatgtttg gtacccatgt gaatgatacg
    11641 ggtaacattt ggcgtttgag aatcaccaga gcggggtaaa tttaaattat gagaggttgg
    11701 tcatattatc gcggggaaac gaagggaggg atttgccaaa gcaatgctgt gccaacgtct
    11761 ggcacatgtt caacgtaggc ccgaaatgac gctttagcgt cgcatcgggc aatctacaaa
    11821 agaggggata acttagtagt aggagtgttc gccgcgctgg tgttcggtga gatcgcgcac
    11881 acctttcagc tccgggaatt cgttcagcag ctgcttctcg atcccttctt tcagcgtcac
    11941 atcgaccatg gaacaaccgt tacagccgcc gccaaattgc agaatggcgt aaccgtcttc
    12001 ggtgatttcc atcagcgaaa cgcgaccacc gtgaccagca agctgtgggt tgatctgcga
    12061 ctgcagcata tactccacgc gctccatcag cggtgcatcg tctgccactt tacgcatttt
    12121 ggcgttcggg gctttcagcg ttaactgaga acccaactgg tcggtaacaa agtcgatctc
    12181 tgcatcttcc aggtatggtg cgcttaactc atcaacatac gcggtcagca ggtcaaattt
    12241 cagggcagtg tcggtggctt ccacggcatc cggcggacaa taagaaacac cacattcagc
    12301 gttaggcgtg ccagggttaa tcacaaatac gcggatttgt gtcccttctt cctgatttgc
    12361 cagcagtttg gcaaagtgcg cttgtgcagc atcggaaata cggatcatag taatggccta
    12421 atagttgact attttagttg gttataatac gcccatcatc gaggctctac aaggttcgac
    12481 aaaggcacca gacctggaca gtcgccgcac cattgcgtaa aagcaactgc gcaatctctg
    12541 cgacggtact tccggtggta acgacatcat ccacaatcac catatggcga ccttgcacgg
    12601 gcaattcaag acgaaaggca tttttcaggt tgcgcttgcg cagccgggca ctgagaaaat
    12661 gctgggtcgc agtggcccgt gtacgtgtga cggcttcgct atcccattgg cagtgcaacc
    12721 agcgtgataa cggctgacac agcaaatcgc tctgattaaa tccccgacgc cagtgacgcc
    12781 gctgccataa cggaacgctg atgatgcgat ccggcaattg caacccggtg gtgcgacgag
    12841 cgtgtaagac ttccaatagt aacagacgtg acagggcgct ggcgatttca ctgcgccggg
    12901 aaaatttaag ctggtggatg agcagactta acggcggcgc atagtcggca accgtgacca
    12961 gtctttgcca gggcggcggt ttttgcaggc agcgaccgca gggaagatgg gagtgtgtgg
    13021 cgggtaatcc acattgtggg cataacgttt tatctgtgcg ggtggcgcgt gaacagaccg
    13081 aacaaatccc ccaatgacct aacgccagtg gcattcggca tagccagcat aatcccggta
    13141 ctgttagcat atgttcatcc ttgtaagtca aaagagaaca atagcggatg aataacattt
    13201 ggtggcagac caaaggtcag gggaatgttc atcttgtgct gctgcacgga tggggactga
    13261 atgccgaagt gtggcgttgc attgacgagg aacttagctc gcattttacg ctgcaccttg
    13321 ttgatctgcc cggcttcggg cgcagccagg gatttggtgc gctgtcactt gctgatatgg
    13381 ccgaagccgt gctgcaacag gcacctgata aagccatttg gttaggctgg agtctgggcg
    13441 ggctggtggc aagccagatt gcgttaaccc atcccgagcg tgttcaggcg ctggtcaccg
    13501 tggcgtcgtc accttgtttt agtgctcgtg acgattggcc ggggataaaa ccggacgtgc
    13561 tggcgggatt tcagcagcaa ctcagtgatg attttcagcg tacagtggag cggttcctgg
    13621 cgttacaaac catggggact gaaacggcgc gccaggatgc gcgggcgttg aagaaaaccg
    13681 ttctggcgtt accgatgccg gaggttgacg tgcttaatgg cgggctggaa atcctgaaaa
    13741 cggttgatct ccg
//
LOCUS       DAJVRF010000112        13260 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJVRF010000112 DAJVRF010000000
VERSION     DAJVRF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.112"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="OQN13_002975"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="OQN13_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCS1616268.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="OQN13_002976"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="OQN13_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCS1616269.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="OQN13_002977"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="OQN13_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCS1616270.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="OQN13_002978"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="OQN13_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCS1616271.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="OQN13_002979"
     CDS             complement(4036..5568)
                     /locus_tag="OQN13_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCS1616272.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACHGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="OQN13_002980"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="OQN13_002980"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCS1616273.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="OQN13_002981"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="OQN13_002981"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCS1616274.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="OQN13_002982"
     CDS             complement(8663..10399)
                     /locus_tag="OQN13_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCS1616275.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="OQN13_002983"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="OQN13_002983"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCS1616276.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="OQN13_002984"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="OQN13_002984"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCS1616277.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="OQN13_002985"
     CDS             complement(12121..12855)
                     /locus_tag="OQN13_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCS1616278.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13056..>13260
                     /locus_tag="OQN13_002986"
     CDS             13056..>13260
                     /locus_tag="OQN13_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCS1616279.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAV"
BASE COUNT         3365 a         3370 c         3489 g         3036 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 accgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgggg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatctggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatggcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc tgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    13141 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca
    13201 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
//
LOCUS       DAJVRF010000113        13102 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJVRF010000113 DAJVRF010000000
VERSION     DAJVRF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13102
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.113"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            270..1388
                     /locus_tag="OQN13_002987"
     CDS             270..1388
                     /locus_tag="OQN13_002987"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCS1616280.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            1454..1702
                     /locus_tag="OQN13_002988"
                     /pseudo
     CDS             1454..1702
                     /locus_tag="OQN13_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            1767..2135
                     /locus_tag="OQN13_002989"
     CDS             1767..2135
                     /locus_tag="OQN13_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS1616281.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2229..2882
                     /gene="nfsB"
                     /locus_tag="OQN13_002990"
     CDS             2229..2882
                     /gene="nfsB"
                     /locus_tag="OQN13_002990"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCS1616282.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            2990..4237
                     /gene="ybdG"
                     /locus_tag="OQN13_002991"
     CDS             2990..4237
                     /gene="ybdG"
                     /locus_tag="OQN13_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCS1616283.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4305..5681)
                     /gene="pheP"
                     /locus_tag="OQN13_002992"
     CDS             complement(4305..5681)
                     /gene="pheP"
                     /locus_tag="OQN13_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCS1616284.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5783..8926)
                     /gene="cusA"
                     /locus_tag="OQN13_002993"
     CDS             complement(5783..8926)
                     /gene="cusA"
                     /locus_tag="OQN13_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCS1616285.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKARENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(8938..10161)
                     /gene="cusB"
                     /locus_tag="OQN13_002994"
     CDS             complement(8938..10161)
                     /gene="cusB"
                     /locus_tag="OQN13_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCS1616286.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(10177..10509)
                     /gene="cusF"
                     /locus_tag="OQN13_002995"
     CDS             complement(10177..10509)
                     /gene="cusF"
                     /locus_tag="OQN13_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCS1616287.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(10533..11915)
                     /gene="cusC"
                     /locus_tag="OQN13_002996"
     CDS             complement(10533..11915)
                     /gene="cusC"
                     /locus_tag="OQN13_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCS1616288.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            12072..12755
                     /gene="cusR"
                     /locus_tag="OQN13_002997"
     CDS             12072..12755
                     /gene="cusR"
                     /locus_tag="OQN13_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCS1616289.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            12745..>13102
                     /locus_tag="OQN13_002998"
     CDS             12745..>13102
                     /locus_tag="OQN13_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCS1616290.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKD"
BASE COUNT         3135 a         3563 c         3235 g         3169 t
ORIGIN      
        1 caaaatggct gtttatttat acagtaaatc ctgtttcgga cttctcactc ctcatttaat
       61 caactgaagc tgagcccgtt ttttctgcat gcctcaatcg tcaaaattct tcgccatcag
      121 ccagctctgc cataacttgc cacattgaaa aagtagccaa attgcttcag tcaccatcat
      181 caaaaaaaga aacattccag aacttccgca tctcgcccag ccgctccgac ttctatactg
      241 aatagaaaac gccaacataa gagaaaccta tgccattacc cgattttcat gtttctgaac
      301 cttttaccct cggtattgaa ctggaaatgc aggtggttaa tccgccgggc tatgacttaa
      361 gccaggactc ttcaatgctg attgacgcgg ttaaaaataa gatcacggcc ggagaggtaa
      421 agcacgatat caccgaaagt atgctggagc tggcgacgga tgtttgccgt gatatcaacc
      481 aggctgccgg gcaattttca gcgatgcaga aagtcgtatt gcaggcagcc gcagaccatc
      541 atctggaaat ttgcggcggt ggcacacacc cgtttcagaa atggcagcgt caggaggtat
      601 gcgataacga gcgctatcaa cgaacgctgg aaaactttgg ctatctcatc cagcaggcga
      661 ccgtttttgg tcagcatgtc catgttggct gcgccagtgg cgatgacgcc atttatttgc
      721 tgcacggctt gtcacggttt gtgccgcact ttatcgccct ttccgccgcg tcgccatata
      781 tgcaaggaac ggatacgcgt tttgcctcct cacgaccgaa tattttttcc gcctttcccg
      841 ataatggccc gatgccgtgg gtcagtaact ggcaacaatt tgaagccctg tttcgctgtt
      901 tgagttacac cacgatgatc gacagcatta aagatctgca ctgggatatt cgccccagtc
      961 ctcattttgg cacggtggag gttcgggtga tggatacccc gttaaccctt agccacgcgg
     1021 taaatatggc gggattaatt caggctaccg cccactggtt actgacagaa cgcccgttca
     1081 aacatcagga gaaagattac ctgctgtata aattcaaccg tttccaggcc tgtcgctatg
     1141 ggctggaagg cgtcattacc gatccgcaca ctggcgatcg tcgaccgcta acggaagaca
     1201 ccttgcgatt gctggaaaaa atcgcccctt ccgcacataa aattggtgca tcgagcgcga
     1261 ttgaggccct gcatcgccag gtcgtcagcg gtctgaatga agcgcagctg atgcgcgatt
     1321 tcgtcgccga tggtggctcg ctgattgggc tggtgaaaaa gcattgtgag atctgggccg
     1381 atgactaagt agggaattgc cttacagaag aatatccctg acaatggtgt tttaacttac
     1441 atttaacaat gttatgaaac accctttaga aaccttgact accgcagcag gcattttgct
     1501 gatggctttc ctctcttgcc tgctgctgcc cgcccccgca ctggggctga cgctggcaca
     1561 aaaactggtg accatgttcc atctgatgga tcttagctag ctttacactt tattgttttg
     1621 tctgtggttt ttagtgctgg gcgctattga gtattttgtt ctgcgcttta tctggcgacg
     1681 ctggttctcg ctggcggatt aaacgtcgat tatcgccgta cttcgcggca tactttgctt
     1741 attctccttc gcctaaagga acgtttatgg ataagcaatc actgcacgaa acggcgaaac
     1801 gcctggccct tgagttaccc tttgtcgagc tttgctggcc ttttggcccg gagttcgatg
     1861 tttttaaaat tggcggcaaa atttttatgc tgtcgtcgga gctgcgcggc gtccccttta
     1921 tcaatctgaa gtccgatcca caaaaatccc tgttaaatca gcagatatac ccgagcatta
     1981 agccagggta tcacatgaat aaaaagcact ggatttcggt gtatcccggc gaggaaatct
     2041 ccgaagcgtt acttcgcgat ctgatcaacg attcgtggaa tctggtggtt gatggtctgg
     2101 ctaaacgcga tcaaaaaaga gtgcgtccag gctaaagcgg aaatctatag cgcatttttc
     2161 tcgcttacca tttctcgttg aaccttgtaa tctgctggca cgcaaaatta ctttcacatg
     2221 gagtctttat ggatatcatt tctgtcgcct taaagcgtca ttccactaag gcatttgatg
     2281 ccagtaaaaa acttaccccg gaacaggccg agcagatcaa aactctcctg caatacagcc
     2341 catccagcac caactcccag ccgtggcatt ttattgttgc cagcacggaa gaaggtaaag
     2401 cgcgtgttgc caaatccgct gccggtaatt atgtgttcaa cgaacgtaaa atacttgatg
     2461 cctcgcacgt cgtggtgttc tgtgcaaaaa ccgcgatgga cgatgcctgg ctgaagctgg
     2521 ttgttgacca ggaagatgct gatggccgct ttgccacgcc ggaagcgaaa gccgcgaacg
     2581 ataaaggtcg caagttcttc gctgatatgc accgtaaaga tctgcatgat gatgcagagt
     2641 ggatggcaaa acaggtttat ctcaacgtcg gtaacttcct gctcggcgtg gcggctctgg
     2701 gtctggacgc ggtacccatc gaaggttttg acgccgccat cctcgatgca gaatttggtc
     2761 tgaaagagaa aggctacacc agtctggtgg ttgttccggt aggtcatcac agcgttgaag
     2821 attttaacgc tacgctgccg aaatctcgtc tgccgcaaaa catcacctta accgaagtgt
     2881 aattctctct tgccgggcat ctgcccggct atttcccctc agattctcct gatttgcata
     2941 accctgtttc agccgtcatc ataggctgct gttgtataaa ggagacgtta tgcaggattt
     3001 aatatcccag gttgaagatt tagcgggtat tgagatcgat cacaccacct cgatggtgat
     3061 gattttcggt attatttttc tgaccgccgt cgtggtgcat attattttgc attgggtggt
     3121 actgcggacc ttcgaaaaac gagccatcgc cagttcacgg ctttggttgc aaatcataac
     3181 ccagaataaa ctcttccacc gtttagcttt taccctgcag gggattatcg tcaatattca
     3241 ggcggtattc tggctgcaaa aaggcaccga agcggcagat attctgacta cctgcgcgca
     3301 gttgtggatc atgatgtatg cgctgctttc agtcttctcg ttgctggatg ttattttgaa
     3361 tctggcgcag aaattcccgg cggcatctca gttaccgctg aaagggatat ttcaggggat
     3421 taaactgatc ggcgcgatcc tggtcggcat tttgatgatc tcgctgctga ttggtcagtc
     3481 gccagcgatt ctgatcagcg gtcttggtgc aatggctgcc gtgctgatgt tggtatttaa
     3541 agatccgatt cttggtctgg tggccggtat tcagctttcc gcgaacgata tgctgaaact
     3601 gggcgactgg ctggagatgc cgaaatacgg cgcagatggc gcggtgatcg atattgggtt
     3661 aaccaccgtc aaagtgcgta actgggacaa taccattacc actattccca cctggtctct
     3721 ggtttctgac tcctttaaaa actggagcgg gatgtcagca tctggcgggc gacgtattaa
     3781 gcgcagtatc agtattgatg tcaccagtat tcgttttctt gatgaagacg aaatgcaacg
     3841 tctgaataaa gcgcatttgt taaagcctta tttaaccagc cgccatcagg aaattaatga
     3901 gtggaatcgc cagcaaggtt ctacggagtc ggtattaaac ctgcgccgaa tgaccaatat
     3961 tggaaccttt cgtgcctatc tgaacgaata tctgcgtaac catccgcgga ttcgtaaaga
     4021 tatgacctta atggtacgcc aactggctcc aggtgataac ggtttaccgc tcgagatcta
     4081 cgcgtttact aacaccgtgg tgtggctgga atatgaaagc attcaggctg atatattcga
     4141 tcacatattt gcgattgtcg aagagtttgg tctgcgactt catcagtcgc caaccggcaa
     4201 tgatattcgc tctctggcgg gtgcatttaa gcagtaatta aaaaaaccgc tctcatcgaa
     4261 tggatgagag cggacatcgt tttgtcggat gtagcgtgaa tgccttattt ccgacgcagc
     4321 gttttaaatg ccacaaacag gaatacaatc cacaccggca gcaggatcgc tgacaagcgc
     4381 atatcatcca tcgtgcacat cagcagcaaa atcatgccga ggaaggcaat gcagagatag
     4441 tttccgaacg gatagagcag cgccttaaac tgtgtttcac gcccctgacg tcgcatcgct
     4501 gcacgaaaac gcagatgcgc cagacagatc ataatccagt tcaacagcag cgttgctacc
     4561 accagcgcca tcagcagacc aaacgctttt tgcggcagca gatagttgat taacaccacc
     4621 agcgaagtga tcgctccgga aagcatcagc gagttaatcg gcacaccgcg acggctgacg
     4681 cgagtcaaaa acttcggcgc attaccctgc acagaaaggc caaacagcat gcggctgtta
     4741 gagtaaaccc cgctgttata cactgacagc gatgctacca gaatgacgaa gttcagcgca
     4801 gaagctacca cgttgctgtc gagattatgg aaaatcatca caaacgggct actgttggat
     4861 ttcacttcca cccacggata gagcgccagt aaaaccacca gtgaaccgat gtaaaacagc
     4921 aggatgcgat acaccacctg atttaccgct tttggaatgc ttttatccgg atcgcgcgct
     4981 tcagcggcag taatcccaat cagctccaga ccgccgaagg agaacataat taccgccagc
     5041 gacaaaatca gcccattcca gccggtggcg aagaaaccac cgtagcgcca gaggttgtcg
     5101 atactggctt tctcgccgcc gtgaccagaa aacagcagcc acaggccaaa gccgatcata
     5161 ccgatgattg ccagcacttt aatcaacgca aaccagaact cggtttcgcc atataagcgc
     5221 acgttcacca ggttaacggc gttgatgata ataaagaagg cggcagccca aatccacgtt
     5281 ggaacatccg ggaaccagta ctgcatatag atgcccgcag cggtcagctc tgccattccc
     5341 accagcacga acattaccca gtagttccag ccagagagga agcccgcaaa cggtccccag
     5401 tatttatagg caaagtgggc aaatgaaccg gataccggct cttcgaccac catctcgcca
     5461 agctggcgca taatcaggaa agcgatgatc ccggcgacgc cgtaacccag caatacagcc
     5521 ggacccgcca tctgaatcgc cggaccaatg ccaagaaaca gaccagtacc aattgcgcca
     5581 cccagcgcaa tcagttgaat atgacggtta tgtaatccgc gatgaagcgt cggctcttga
     5641 ttcgacgcag tatcttccga tacggttgac gcgtttttca cgcctttccc ctgtgtgtct
     5701 tttttgttga ggggcacctt ttaacattta gtgcccatcg tcgcaagaca caatccacac
     5761 ggttaaacgg ggtatcctgc ttttatttcc gtacccgatg tcggtgcagc cacatcagct
     5821 tatacgccgc cgggataata aacagcgaca gcaaaggtgc ggtgatcatg ccgccaatca
     5881 tcggtgcggc aatccggctc atcacctctg aaccagcccc cgttccccac agaatcggca
     5941 gcagaccggc gataatcacc gccaccgtca tcgctttcgg gcgcacgcgc agtaccgcgc
     6001 cgtgatacaa cgcctcatcc agcttctgct cactgaatgt ttgcggatta ttcaacgacg
     6061 gctcggcctc tatggcgtga cgtaaataca tcagcatcac cacgccaaat tcggcggcga
     6121 ccccggcaag ggcaataaaa ccggtgcccg ttgccacaga aagatgaaag cccatccacc
     6181 acagcaacca gatgccgccc accagcgcaa acggtacgct gctgataatc agcaacgctt
     6241 cgcccacccg acggaatgcc agatacaaca gcacgaagat gatcatcagt gtcatcggca
     6301 ccatgagttt cagcttatgg ttggcgcgct ccagtagctc gaactgcccg gagaatgcca
     6361 cgctggtgcc cggttttaac tgcacttttt cagctatcgc tttttgcaaa tcgtgaacca
     6421 ccgacaccat gtcacgatcg cgggcatcaa tataaatcca gctcgtcggg cgcgcattct
     6481 cggttttcag catcgacggt ccggtagaga ctttaacgtc ggccacgtct gccagggtga
     6541 tttgctgctt catcggcgtc aggatcggta gctggcgcag tgcctgcggg ctatcgcgcc
     6601 agctttgcgg ataacgcaga ttaatgggat aacgggcaat cccttccacc gtttcgccaa
     6661 ccatcgcccc gcccaccgca gaagtcacaa acaactgcac atccgccacc gtcataccgt
     6721 aacgcgcggc tttttcacgg ttaatctcaa cgttgatata gcgcccacct tccagacgct
     6781 cggctagagc agaagccacg cttggcaccg ttcgcgctac ttcttcaatt tgctcagcca
     6841 tcgcgtcgat atccgccagc acagtgccgg aaactttaat gccgatgggg cttttaatgc
     6901 cggttgagag catatcgata cggttacgaa ttggcggcac ccacagattc gccagccccg
     6961 gcagccgcac ggtgttatcc agttcctcaa tgattttgtc catcgtcatg ccaggccgcc
     7021 actgatcctg cggcttaagc tggatggtcg tttcaaccat ctcgagcgga gcagaatcgg
     7081 tggcggtttc cgctttcccg gttttgccaa atacccgcgc cacttcaggt acgctcataa
     7141 ttagcttgtc ggttttttgc agcatactcg ccgcctctgc tgcggaaatc cccggcagcg
     7201 tcgatggcat atacaacaag tcgccttcat tgatctgcgg taaaaattcc ccgccaactt
     7261 tattgagcgg ccagagaacc gtcagcaccg aaagcgctgc caccaacagc gtggttttcg
     7321 gccagtgcag tactttcagc aatagaggat gataaacacg aatcaaaaag cgattgagcg
     7381 ggttactgct ttccggcgga attttgccac ggatccagta ccccatcagg atagggatca
     7441 ctacgatcgc cagcagcgcc gcgcccgcca tcgcataggt tttagtgaac gccaacgggc
     7501 caaacagacg cccttcctgc ccttccaggg tgaagatcgg gataaacgac aacgtgataa
     7561 tcagcagact gataaatagc gccggaccca cttcaacaga tgcatcggtg atcacctgcc
     7621 agcgcgtttt attatccagc gtggcgtcag gatgctggtg ctgccactct tccagccgtt
     7681 tatgtgcatt ttcgatcatg acgatagcgg catcgaccat cgccccgacg gcaatcgcaa
     7741 tgccgcccaa cgacataata ttggcattca gtccctggaa gtgcatgaca ataaaagcaa
     7801 tacacaaccc cagcggcaac gaaataatcg ccaccagcgc cgagcgcaca tgccagagaa
     7861 acagcgcaca gactaccgcc accacgataa actcttccag caacttgccg ctgaggttgt
     7921 cgatggcgcg gtcaatgagc tggctgcgat cgtatgttgt aactatctcc acgccttccg
     7981 gcagactact tttcagcgtt tccagtttgt ccttcacgga ggcgatcact tctcgggcgt
     8041 ttttgccgga gcgcaggatc accaccccgc ccgccacttc gccttcgcca ttcagttcgg
     8101 caatgccccg gcgcatctcc gggccaatct ggaccttcgc aacatcacgc agataaacgg
     8161 gcacgccatt ttctctggct tttaaaacga tgtgattaaa gtcgtcgagc gtttgcagat
     8221 agccgctggc gcgcaccata tattccgctt ccgccagttc gatcgacgaa ccgcccgctt
     8281 cctggtttga agcatccagc gcgcttttta cttcggctag actgatgcca tattgcgcca
     8341 ggcgctgggg atcgataacc acctgatact ctttcaccac gccgcccacc gacgccactt
     8401 ccgcaacgtc agggatggtt tttagctcat atttgagaaa ccagtcctgc aatgagcgta
     8461 aatcggccag atcgtgctta ccgctgcgat ccaccagtgc atattcatag atccagccaa
     8521 cacccgtggc atctggtccc agctcggcgc tgactcccgc aggcagctta ccctgtacct
     8581 ggttgaggta ctccagcacc cgcgaacgcg cccagtacgg atcggtacca tcttcgaaaa
     8641 tgacatacac ataagagtct ccaaactgcg agaaaccgcg cacagtcttc gcgccaggca
     8701 ccgacaacat ggtggtggtt agcggataag tcacctgatt ttcaacgatt tgcggtgcct
     8761 gaccgggata gctggtttta ataatcacct gcacatcgga gagatccggc agcgcatcca
     8821 ctggcgtgtt aatgatggtc caggtgcccc agatgctcag aaacaacgcg cccatcagca
     8881 ccaaaaaacg gttcgccacc gagcgacgaa taatccattc aatcattggt tattccctca
     8941 atgcgcattg gtagcacttt cagagcgcat ccgctccagt gcgccagaaa tattggcttc
     9001 ggaatcaatc aggaacaggc cgctggaaac caccttttca ccttccgcca gaccagagtg
     9061 taacgcggtg acgccttgcg atgcctggaa aacggcaacg cgtttcggta caaagcgtcc
     9121 gtcggcatca acggtaatca cccgctgttc gttgccggta tcaatcagcg cctgtgacgg
     9181 aatgagcagc atcggttcgc tggcggtgtt gagttgcaac caggcgttca tgcccggttt
     9241 tagcgcctcg tcggcgttgt cgacttccag acgcagctgc agcgtgcggg tcgcggcatc
     9301 cacgccaggt agcagcgtcc atttgcggat ggtgagtgtt ttatccggtc gcgccggaac
     9361 ggtgagcgta aactgcgagg catctttcac cagccaggcg atagactccg ggatcgcagc
     9421 agtgacccac accgggtcca taccctgaat tttcgctacc acgttatctt tggcgatatt
     9481 cattcccgcg cgcagatcaa acgcggtgat cacgccatca atgggtgctt tgagcgtaaa
     9541 gcgagtctgg attttttgcg tggcgatcag gcggcgaata tccgcctccg gcattcccgc
     9601 cagtcgcagc cgctcaagaa tgccttcagt ctgggtcgcc gtaccgccgg tttcgcgcag
     9661 cagtaaatac tcactctgcg cttccaccca gtcaggaatg gtcaggtcga gaagcggtgt
     9721 gcccttttgt actttatcgc ccacggtaag cggatacacc ttgtcgataa acccggcagc
     9781 gcgagcctgc acaatggcat actgatactc gttgtaactg acattcgccg ggaaactctg
     9841 ggcaaaagtc agcggtccgc gcgtgacggt agccgttttc acccccagat tctgcgtctg
     9901 agtcgggtca atgcgcacac cagacgcaga actctcttca tcggcatatt tcggcaccag
     9961 atccatatcc ataaacggcg atttacctgg tttatcgaac cgcgtattgg ggtacatcgg
    10021 gtcgtaccag aataagattt tacgttctgc ggtcgacgtt ttttctgcgg gtggttcagc
    10081 ctttgcaaac caggtaaaac ctgccgcaga aataataccg cccgcgatca tgctgccgat
    10141 aataagcgcg atttttttca tctcattaaa cctgggttac tggctgactt taatatcctg
    10201 taataaagaa aggttgccct gctggacaaa attaaacgcc actttgtcgc cggttttaat
    10261 ttcactcatt ttcgtctgcg gggtgatggt aaagcgcata gtcatctccg gccagttcac
    10321 ggcagcaatc ggatcgtgat ggatggtgat ttttttgctt tccagatcaa taccctttac
    10381 cacgccagtg gcgctaataa cctgcggttg tacttcgctc atggtttcat gatgatgttc
    10441 gttagcctgg gcattaaagc caataacggt aaacagactg aacattgcga cttgcagtgc
    10501 ttttttcatt tattctctcc tggagttaaa aattattgct gccaaccgcc ccctaaggcg
    10561 gtatacaaag aaatttcgtt aacctgacgg gcataattca ggtcgagtaa agtttgtcgg
    10621 gttgcaaata aagaacgctc ggcatccagc acttccagat aacttaccgc gccgtgctga
    10681 tataatgccc gcgcccgttg caaagtaatt tgcagcgacg ccagataacg ctgctgggcg
    10741 ctgatttgat cgttcaggct ttgacgtaat gccagcgcat ctgccacttc tttaaaggcg
    10801 ttctggattt tctgttcata attcactacc gactgctgct ggcgaatttc ggcgatatcc
    10861 agattggcct ggttgcgtcc ggcattaaaa atggggatct caattttagg aataaaattc
    10921 cacatcccgc tgctagcgtt aaataatgaa gacagatcgc tgctggcggt tgatattccg
    10981 ctggtcaggc tgatagacgg gaaaaatgcc gcacgcgcag caccaatatt agcattagcc
    11041 gccattaacg cgtgttcagc ttccataata tccgggcgct gcaataagat ttgcgacgac
    11101 aagcccgccg gtaatttaac gctttgcagg ctgccgctgt ttactgtctg cgcttgcggc
    11161 agtttgccgt agcttcccaa taacagttgc aatgcattat tcgcctgcgc cagttccccc
    11221 tgacgtttag cgatgtcact gcgggtactt tctatcaccc cgcgagcctg ttccagcgcc
    11281 agaacattgc tgctaccggt caacagttgt ttttcgacaa acgcatatga ctgctgataa
    11341 ttacgcagcg tttcttcggc tatttgcaat tgcgcatacg ccagttgctg attgaaatag
    11401 ctttgcgcga cattagaaac cagcagaata tgcaccgcgc gctgagcttc ctcagtggct
    11461 aaataatttt gtcgctcggc ttcgctcatg ttctttaagc gaccgaaaaa atcgaggtca
    11521 aagctggcgt taaggccggt cgaaaactcc cgtgtcgtgg cggtattccc tttaagatta
    11581 ccgctccagc tgccgctgcc ctcgccattg agctgtgggt agcggtcggc atcggtcaga
    11641 cgatattgcg cccgtgcctc ctgcactttc agcgtcgcca tgcgcaaatc ccggttattc
    11701 accagcgcct cgctaatcag cgtcttcacc tgattatcaa caaaaaaggt gcgccagccc
    11761 acgttctggt agttatctgc tgcgttaacc aagccgttct ggctgagtga gaactgctgc
    11821 ggcacgggca ttgccgtacg ctgataatcc ggtgccagtg aacaaccggt tagcgcaagg
    11881 gccacacaaa atggcagaag tttacaagga gacataggct cataatttct ggtgatttta
    11941 taccgccaac tttactcgcc aggctctgat tttccggtga caggaaaatg acaaaattgt
    12001 cattttgcca ataagcgatt gccatctgat cccgctactc tagaattgcc cgggcaacat
    12061 gcggaggaaa aatgaaactg ttgattgtcg aagatgaaaa gaaaaccgga gaatacttga
    12121 ccaaagggtt aaccgaagcc ggttttgtgg tcgatttggc cgacaacggg ctgaatggct
    12181 accatctggc gatgaccggt gattatgacc tgataatcct cgatattatg ctgccggacg
    12241 tgaacggctg ggatatcgtg cgcatgttgc gctccgccaa taaagggatg ccgattctgt
    12301 tgcttaccgc gcttggcacc attgaacatc gcgtcaaggg gctggagttg ggggccgatg
    12361 actatctggt gaagccgttc gcttttgctg aactgctggc gcgggtgcgc actctcctgc
    12421 ggcgcggggc ggcggtgatt atcgaaagtc agtttcaggt tgccgatctg atggtcgatc
    12481 tcgtcagccg caaagtcacc cgcagcggca cgcgcatcac tctgaccagt aaagagttta
    12541 ctctgctgga gttttttctc cgccatcagg gcgaagtgct gccccgctcg cttatcgcct
    12601 cgcaggtgtg ggacatgaat ttcgacagcg acactaacgc cattgatgtg gcggtgaagc
    12661 ggctacgcgg caaaatcgac aacgactttg agccgaagct gattcagacc gtgcgcggcg
    12721 tgggctatat gcttgaggtg ccggatggtc agtaagccat ttcagcgccc gttttcgctg
    12781 gcaacccgtc tgaccttttt tatcagcctg gccaccatcg cggcgttttt cgcctttgca
    12841 tggatcatga tccactcagt gaaagtgcat tttgccgagc aggatattaa tgatttaaaa
    12901 gaaattagcg ccacccttga acgggtactt aatcatcctg acgagacgca agcccgacgc
    12961 ttaatgacgc tggaagatat cgtcagtggt tattccaacg tgttgatttc cctggcagat
    13021 agccacggca aaacggtgta tcactccccc ggtgcgccgg atatccgcga gtttacgcgt
    13081 gacgccatac ccgataaaga cg
//
LOCUS       DAJVRF010000114        13010 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJVRF010000114 DAJVRF010000000
VERSION     DAJVRF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13010
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.114"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..807
                     /locus_tag="OQN13_002999"
     CDS             <1..807
                     /locus_tag="OQN13_002999"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1616291.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            1177..2001
                     /gene="gadX"
                     /locus_tag="OQN13_003000"
     CDS             1177..2001
                     /gene="gadX"
                     /locus_tag="OQN13_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCS1616292.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            2369..3097
                     /gene="gadW"
                     /locus_tag="OQN13_003001"
     CDS             2369..3097
                     /gene="gadW"
                     /locus_tag="OQN13_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCS1616293.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(3460..6573)
                     /locus_tag="OQN13_003002"
     CDS             complement(3460..6573)
                     /locus_tag="OQN13_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCS1616294.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(6598..7755)
                     /gene="mdtE"
                     /locus_tag="OQN13_003003"
     CDS             complement(6598..7755)
                     /gene="mdtE"
                     /locus_tag="OQN13_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCS1616295.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7815..8093
                     /locus_tag="OQN13_003004"
     CDS             7815..8093
                     /locus_tag="OQN13_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616296.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(8094..8621)
                     /gene="gadE"
                     /locus_tag="OQN13_003005"
     CDS             complement(8094..8621)
                     /gene="gadE"
                     /locus_tag="OQN13_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCS1616297.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(9420..9992)
                     /gene="hdeD"
                     /locus_tag="OQN13_003006"
     CDS             complement(9420..9992)
                     /gene="hdeD"
                     /locus_tag="OQN13_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCS1616298.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            10247..10579
                     /gene="hdeA"
                     /locus_tag="OQN13_003007"
     CDS             10247..10579
                     /gene="hdeA"
                     /locus_tag="OQN13_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCS1616299.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            10695..11021
                     /gene="hdeB"
                     /locus_tag="OQN13_003008"
     CDS             10695..11021
                     /gene="hdeB"
                     /locus_tag="OQN13_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCS1616300.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            11085..11732
                     /locus_tag="OQN13_003009"
     CDS             11085..11732
                     /locus_tag="OQN13_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCS1616301.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(11774..12304)
                     /locus_tag="OQN13_003010"
     CDS             complement(11774..12304)
                     /locus_tag="OQN13_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1616302.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(12460..>13010)
                     /gene="slp"
                     /locus_tag="OQN13_003011"
     CDS             complement(12460..>13010)
                     /gene="slp"
                     /locus_tag="OQN13_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCS1616303.1"
                     /translation="ALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQPGLYVG
                     QQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFLDPVNYR
                     NHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYGYGAFWP
                     EPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3546 a         2944 c         3000 g         3520 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgttgctg
      721 gaagactaca aagcctccct gaaatatctc agcgatcacc cgaaactgca gggtattgcc
      781 cagcagaaca gctttaaaca cacctgataa cataacgttg taaaaaccga atgcccagcc
      841 tttaaaaaaa cagctgggca ttcggttgct tattaatcgc aataatatat tggctgtcta
      901 ttcatcgtgt tgataagata tatgttatat gaatgtttat taaatgtagc gattgccttt
      961 aatgtcacga aaagggatta tttgcttgct attaatttca ctgcgttctt aaacacattt
     1021 ttgtattata aatccagtca tcctgcccgt agaacatcac acattatcat cctgttctcc
     1081 cgcttcgttt aaatttattt atcaatcaat ttgacttaag agggcggcgt gctacattaa
     1141 ttaacagtaa tatgtttatg taatattaag tcaactatgc aatcattaca tgggaattgt
     1201 ctaattgcgt acgcaagaca taaatatatt ctcaccatgg ttaatggtga atatcgctat
     1261 tttaatggcg gtgacctggt ttttgcggat gcaagccaaa ttcgagtaga taagtgtgtt
     1321 gaaaattttg tattagtatc aagggatacg ctttcattat ttctgccgat gctcaaggag
     1381 gaggcattaa atcttcatgc acataaaaaa atttcttcat tactcgttca tcactgtagc
     1441 agagatattc ctgtttttca ggaagttgcg caactatcgc agaataagaa tcttcgctat
     1501 gcagaaatgc tacgtaaaag agcattaatc tttgcgttgt tatctgtttt tcttgaggat
     1561 gagcacttta taccgctgct tttgaacgtt ttgcaaccga acatgcgaac acgagtttgt
     1621 acggttatca ataataatat cgcccatgag tggacactag cccgaatcgc cagcgagctg
     1681 ttgatgagtc caagcctgtt aaagaaaaaa ttgcgcgaag aagagacatc atattcacag
     1741 ttgcttactg agtgtagaat gcaacgtgct ttgcaactta ttgttataca tggcttttca
     1801 attaagcgag tcgcagtgtc ctgtggatat cacagcgtgt cgtatttcat ttacgtcttt
     1861 cgaaattatt atgggatgac gcccacagag tatcaggagc gatcggcgca gggattgccg
     1921 aaccgtgact cggcggcaag tattgttgcg caaggaaatt tttacggcac tgaccgttct
     1981 gcggaaggaa taagattata gaattttact cagacataaa aaaaacccgg cataggggac
     2041 cgggaagagg atagtctgcc gtctccagac taataaaccg ttataacact ccctgttggc
     2101 acgggaaact ttgtgctctc agtaagttaa atataacttt tactggaaat aagatcagcc
     2161 atttttttat aaacataagc tatacgctgt gcgaaaatat attcgtgctg catttactta
     2221 ttatcaatta actgttatgc aaaactactt tgtggataaa ttttggtcct accaaatctg
     2281 gcagtttttt cgctaagaaa cagtctggca acatttcatt agtatactga aattgaaata
     2341 atcgcagtat gaaatataag ggataatcat gactcatgtc tgctcggtga tcctcattcg
     2401 tcgttcattc gatatttatc atgaacagca taaaatatcg ctgcataacg agagtatcgt
     2461 gctgctggag aaaaatttgg cagacgattt tgcgttttgt tcaccggata cgcgacgact
     2521 ggatatcgat gagctgacag tttgccatta cttacaaaat attcgtcagc taccacgcaa
     2581 tttagggtta catagcaaag accgtctatt aattaaccag tcacccccca tgccgctggt
     2641 gacggcgatt tttgatagct tcaatgaatc cggggtaaat tcaccgatac tgagcaatat
     2701 gctctacctt tcctgtttat cgatgttttc tcataagaaa gaactgatcc ccttactttt
     2761 caatagcatc agcactgttt caggaaaagt tgaacgcctt attagctttg atatcgccaa
     2821 acgttggtat ctgcgcgata tcgcggaaag aatgtatacc agcgagagtc tcataaaaaa
     2881 aaagttgcag gatgaaaata cctgtttcag taaaatatta ctcgcttcca ggatgtcgat
     2941 ggccagacga ttactcgagt tacgtcaaat tcctctgcat actattgcag aaaaatgtgg
     3001 ctatagcagt acgtcgtact ttataaacac atttcgacaa tattatggtg taacgccaca
     3061 tcagtttgcg caacattcgc caggtacctt ttcctgacat attttgcatt tgaatattgg
     3121 tcaggatctc acacctgctt catgtgaaac tcttccctga tgatttctgc cgggctaccg
     3181 gctagttctc tttcgcagtt tgccaggcgg tataggtatt ttcaggagga aaagactcgg
     3241 catgtttggg attattaagc tgacaattca tacgattacg ggaatgtggg tcagtattgt
     3301 gctgttcaaa ttgatgacga atggctggag tggtttctac tttcagtgct gtgtactaag
     3361 cctggtattc ctgacggtta gctggttgtt gagtggtgaa tggttagcag gaaagagtaa
     3421 ggctgaacct tcacgttcaa ccttactctc atttacacgt tacgcttttt taaagcgggc
     3481 aaagagatgt tccactacaa cgaaaaagac cggaacgaag taaattgcca gcactgttgc
     3541 ggcaaacatc ccgcccatca cgccggtacc taccgcgttt tgcgcgccag aaccggcacc
     3601 atgactgata accagcggca gcacgccgag aataaaggcg agagaggtca tcaggattgg
     3661 gcgtaaacgc atccgcgccg cttcgatgat tgcctctatc ggcgttttcc cttctttctg
     3721 catcatctca acggcaaatt cgacaatcag gatggcgttc ttggcggaaa gcccgatggt
     3781 ggtcagcaaa ccaacctgga agtagacgtc attacttaag ccgcgcagat cggtggccag
     3841 taatgcgcca acgacgccta acggaacaac caacatcacc gagaacggaa ttgaccagct
     3901 ctcatagagt gcggcgaggg cgaggaacac cacgaccagt gaaatggcat acagtgcagg
     3961 agcctggttt gaggataacg cttcctgata cgacagtccg gtccatgagt agccgacgcc
     4021 tgccggaagt ttagcgacca ggtctgccat aaatttcata gcgtcaccgg tacttttccc
     4081 ggccgccgct tcacctaaaa tctccattga cgggatgccg ttgtagcgtt ccaatcgcgg
     4141 tgaaccatag gtccattcgg tagacgagta ggcagaaagc ggtgccatcg tgccagaggc
     4201 gttgcgtaca taccattggt tgatgttatc cggcaacata cggaacggcg tgcctgcctg
     4261 gacatacact tttttcaccc gcccctggtt gaggaagtcg ttcacgtagc tgctgccgaa
     4321 ggcggtggaa attgtctggt tgatatcaga cagcgccaca cccattgcct cagctttcgc
     4381 ggcgttgacg ttcactttga acatcggcgt atcttccagg ccgttcgggc gtaccccggt
     4441 gacctggtca ggcgattgcg ctgccagtga taacagctcg tttcgtgcct gggttagttt
     4501 ttcgtgcccc aggttaccgt tgtccagcag ttccatatca aaacctgacg cggtacccag
     4561 ttcagccacc gcgggtaagt tgaacgggaa gacgacggct ttattgatac tgcttaacgc
     4621 aatcattgcc cgctgaatga tcgcggtaac cgagttttcc tcaccgacac gttcagacca
     4681 cggcttgaga ctgataaacg ccaggccgtt gttttgcccc tggccgctga agccaaagcc
     4741 gccaacggta aacaccgact gaacattatc tttctctttt gtaagataat aatccgtcac
     4801 ctgttgcagc actttcgtgg tgttaaccat ggtggcaccg gaaggtaact gcgcggtggt
     4861 cataaatacc ccctgatcct cttctggtaa gaaagaggtc ggtgtacgca ggaacagcac
     4921 cgccatcccg gcgcaaatca gcaggtagac caccatgtag cgaccggtac aacgcaacag
     4981 cgagcgggtg ctatcggtat agtgctgagt tgatttttca aacagcgtgt tgaagcgtgc
     5041 gaacagggcg ttaggtttgt gaccgccttc cggcgcggct ttcagaatgg tggcgcacag
     5101 ggcaggggtc aggctcattg ccacaaatac tgaaagcagc atggaggaga tcagcgtgat
     5161 ggagaactgg cggtagatct ccccggttgc gccgctcata aaggccatcg gcataaacac
     5221 tgcggaaaga acaacagcaa taccgaccag cgcacgttgg atctgcccca tcgatttatg
     5281 cgtcgcttcc ttcggtggta gcttatcttc cgcaatgaca cgctcgacgt tctccaccac
     5341 cacgatggcg tcatccacca gtaaccctat cgccagcacc atcccgaaca tagttaacgt
     5401 gttgatggtg aaaccgaccg ccgacaagat cgcaaacgtc ccgagaataa ccaccggtac
     5461 ggcaatcgtc gggatgattg tggcacggaa attctgcaaa aacagataca tgaccaggaa
     5521 gacgaggatg atagcctcaa ccagtgtttt aaaaacttcc tgaatagaaa tttcgataaa
     5581 cggcgtggtg tcgtaaggat aaaccgtctt cagacttgcg gggaaatagg ctgataagcg
     5641 gttcagctcc tctttgactg cccgcgaggt atccagggcg tttgctccgg tagccagttt
     5701 gatggcgatc ccggcagcag gtttgccgtt atagcgcgcc acggtggaat aatcttccgc
     5761 tccaagttcg acgcgggcga catcacgcag cagcacttgc gaaccatctt gctgaacttt
     5821 caacaggatt ttgccaaatt cttccggcgt ttgcagacgc gtctgcacaa tgatcgaggc
     5881 gtttagttgc tggtctgccg cctgtggcat gccacccagt tgaccaccgg aaatctggtt
     5941 gttttgcacc ttaatctggg aaataacatc ggaaggtacc agattgtatt tattgagttt
     6001 ttgcgggtcc agccagatac gcatggcgta ctcggaacca aagagctgta cgctaccaac
     6061 gcccgcggta cggcttagcg ggtctttgat attagacgct acatagtccg cgatatcgta
     6121 ctggttgagg ctgccgttat cagaaataaa cgccgctacc atcagaatat tactgctcga
     6181 cttatcaacg ctaatcccct gctgctgcac cgcttcaggt aatgaaggca tagcgagttg
     6241 cagtttattt tgcacctgaa cctgtgcgat atcaggagat gtcccagtct cgaaggtcag
     6301 agtgatagag gcattacccg ccgcatcact ggttgaagac atgtacatca ggccatcaag
     6361 cccattcata ttttgctcaa tcacctgagt gaccgagtct tctaccgttt gcgcatcggc
     6421 acctggatag gtagcgctga cggtaatggt cggtggcgca atctgcggat actgcgcaac
     6481 cggtaagttc atgatcgcca gaccacctgc aagcatcata ataatggcaa gtacccaggc
     6541 aaaaaccggg cgatcaataa aatagttagc catgaaagtc cccttaagcc tgcaacgtta
     6601 ttgtttcgat tcggtgctgg cgttttcctg gctggaggaa attgctcgtg ctttgatacc
     6661 cggacgaatg cgttgcaaac cggaaacgat cacccgatcg ccagcctgca agccagaggt
     6721 gacaacccac tggtcgccga tggctttgct ggcttcaatt tcgcgtagct gcacaacatc
     6781 gtctttatcc agaatgagcg ccgttgcttt accctgggcg ttgtgagtga cgccttcctg
     6841 cggcaccagt aatacattct ggcggctacc ttcatccact aatgccgtga cgtacatgcc
     6901 aggcagcaag tcaccatttg ggttggggaa aatcgcccgt aacgtcacgg agcccgtggt
     6961 ttcatccacc gtcgggtcgg agaatttcag tgtgccggtc tggctgtagc gtttaccatt
     7021 ttccagattg agctgtactg gcgtactgcc ctgaacctgt ttgatttgcc cactggcgac
     7081 ctcttctttc atgcgcaaga aatcctgcac cgactgcgtg agatcgacat aaatcgggtc
     7141 cagacgttgt acggtaacca gcgaatctgc ctgattagcg gtaacgagtg cgccgacggt
     7201 caccgacgat ttcccgctga cgcccgtaat cggcgaggtg acattcgcgt attgcagatt
     7261 gatcgtcgcc tgttcaacag ccgctttggc gacggtgaca ttggcttctg cttcattcag
     7321 ctgggtgcgc gcggtgtcgt aatcctgacg gctaacgtag ttggtcttca gcaacgatgc
     7381 ctggcggtta aaggtgatgc gggcattgct ggcggtagag agcgctttcg ccagcgagcc
     7441 tttggcggag tttagctcgg cctgtaaagg tgcaggatca atctgataca gcgaatcgcc
     7501 ctggttcact ttatcgcctt cgataaagtt gcgtttaatg ataataccgc ccacctgggg
     7561 acgtatctcg gcaacttcat aaggaacggt tctaccgggc aattcgctca acacattgac
     7621 cgaaccgggg gagagtgtga cgacaccgac ctcaggcgtc atggcggcgg cgttttccgc
     7681 cgatttgtca tcgcaggcgg tgagcatcgc gccgcagaat aacaacggta ttaacagctt
     7741 tcttcttctg ttcattttag tccctgaaaa ttcttgagaa ataacggttt tttaaattgc
     7801 gcgcgaggag agatatgagg gatttacaga ccagcggcat agtggggttg tcagccagta
     7861 aggtgggata caggcacagt gatcgacatg gtgaggtcaa cgacaaaaaa aatgttgccg
     7921 ttctgccaac agttcctgcc agcattcggg caacgaaggg ttctactggt ggatacacat
     7981 acctggggaa taaagtcgtt ttagcctctc ctttagtaat caccgggggt aatgaaatca
     8041 gcatctgcat ccctcgtcat gccagccatc aatttcagtt gcttatgtcc tgactaaaaa
     8101 taagatgtga tacccagggt gacgatgtcg ctcatacgtc ttagtttcag cttgtacata
     8161 atgttgaatt tctgaatctt taatgtttgt tgcgaaatat tgagcgtcga cgtgatattg
     8221 ctttcatttt tatttttcag aatcagttgc ataattttga actgattatg tgaaaaacat
     8281 aattgtgaat tcttatgggg caagtgttta ccataagtaa tctcaagaag atttttgata
     8341 ctttctttgc ggctaacaaa ataaataggc gctttagctt ttagtttact gatgtgataa
     8401 ggtgccaaaa caataattct ctcggcatct aatttctcca gaaatttaat cgcttcttca
     8461 tcaaggatat gattgtgata ggtatcaatg ataaccttga agggtttatt gcctactttt
     8521 ttgatctctg acagggaatt gatttttatc atttcgtgat tatctttcaa ctgccaaaag
     8581 ccctgtaaaa gaaaagaatc tttcgtcatg agaaaaatca taacttgctc cttagccgtt
     8641 atcgtttcga acatgtcatc cttttcatta tttacatcct tgtccgaatc gttgttcaat
     8701 atagtaaacg cctataagaa ctttaccctt cgtgaactaa tacgtctatt aacactaatc
     8761 aatacttcag atttatccat cacgcactcc atatattgat cctaagtaag attcctggtt
     8821 gttatcagct tgtaaatagt aaacaaaaag ttaacgtttg cggattactg cccaagaata
     8881 agttatcaat aaccagtttg tgatctctga agaatattac taaagttaaa atgtaatccg
     8941 atttaaatat cgagtctcct tgtttcgact taagctggca attggattgc cagctttctt
     9001 ttaacgcatt gaaattacct gagtaaataa cgcattcatg tcttcaggtt tttatatagc
     9061 gtctggagac acggatattt atgcaatgta atcaacgcca gttgcaaaaa aatgaacccg
     9121 gattacttat ttaaccgccc atagcatcaa cgcttacgga actctttgtt cactttccca
     9181 cagcactttt aaaacattaa acctgactac ggaaaatatc agccatgata atgtttgaaa
     9241 tggctaattt gccatggagt gaaaaaaatt agatgaaatt cagtaggttg aaataatcac
     9301 tagcaggtaa ttatttcaat gatagtgcgc aattgatcta caacactccg tagcggagag
     9361 agtattaatc ggatcatagt catatcaagt gactatgatc cgggtgacaa ccgggataat
     9421 tattgctgct taacgaacaa actggcgaag ctgaacaggc tggcggcgct aaatatcagt
     9481 tcaattccca ccagtgtgga aaccagcgtt acagacacca tcggcgttgc accaaggaat
     9541 atccaggcaa tgacgatatc cagcacacca ataacgagct gtagccagct gcctttcatt
     9601 gaacgctgac gataccaact catcaggcga ataacccctg caacacagaa caaaccggca
     9661 ataaatgccg caatggcaaa aatgcccagc tccggtgcgc ggatgaagaa atagccgatc
     9721 aataaatagg cgactgcgac gaggaaaccg gataataccg gccagaaatt atgactgcgg
     9781 ttgctgaata acccgacaat aagcgcaatg cccgagcaga ttaataatgc acccactact
     9841 gtgcttaaaa tatcgccaga gacgaacggg aaactgatac acagcaaccc gacgataaac
     9901 agtagcacgg caataaactg gattgctctg cgatgttttt taagcatctc cagatcaaac
     9961 ttcaaaattg ttgccttatc tatatataac atagaaccac cctataaaat taagaagaaa
    10021 atcccctgct atcaatctat gccaaaaacg cgtctaagaa tgtagtcgat ttaataaaaa
    10081 tttcctaatt acagtatctg atgcatctgt aactcattgt attgaaataa aaatatctga
    10141 ttttgatatt ttatatcaac atgacatata cagaaaacca ggttataacc tcagtgtcga
    10201 aattgattcg tgacggctct ttcactttat agttgaggat attacgatga aaaaagtatt
    10261 aggcgttatt cttggtggtc tgcttcttct gccagttgtg agcaatgcag cggatgcgca
    10321 aaaagcagct gataacaaaa aaccggtcaa ctcctggacc tgtgaagatt tcctggctgt
    10381 ggacgaatcc ttccagccaa ctgcagttgg ttttgctgaa gcgctgaaca acaaagataa
    10441 accagaagat gcggttttag atgttcaggg tattgcaacc gtaaccccag ctatcgttca
    10501 ggcttgtact caggataaac aagccaactt taaagataaa gttaaaggcg aatgggacaa
    10561 aattaagaaa gatatgtaat tccgggaatg cgttacatcg tacttccttg catattgaac
    10621 aggccggaat atcttcttta aaagcagcta ttcctcctgt tcatatataa tctctatatt
    10681 gaatgggtta caaaatgaat atttcatctc tccgtaaagc gtttattttt atgggcgctg
    10741 tagcggcttt gtcactggtg aacgcacaat ctgcgctggc agccaatgaa tccgctaaag
    10801 atatgacctg ccaggaattt attgatctga atccaaaagc aatgaccccg gttgcatggt
    10861 ggatgctgca tgaagaaaca gtatataaag gtggcgatac cgttacttta aatgaaaccg
    10921 atctcactca aattcctaaa gtgatcgaat actgtaagaa aaacccgcag aaaaatttgt
    10981 ataccttcaa aaatcaagca tctaatgact tgccgaatta atgaggtaca agtaaaaagg
    11041 agtagcaagt tgagccatct tgctgctcct ttttgcattt ttatatgaca gcagaattta
    11101 ttatacgtct tatactcgcg gcaattgcct gtggcgctat tggcatggaa aggcaaatgc
    11161 gcggcaaagg agcagggtta cgcacacatg tattaattgg catgggaagc gccctgttta
    11221 tgattgtttc gaaatatggt tttgctgacg tgctgtcttt agatcacgtc ggactcgacc
    11281 ccagccgtat tgctgctcag gtggtgacgg gcgtcgggtt tatcggtgca ggtaacattc
    11341 tcgttcgtaa ccaaaatatt gtaggtctga cgacggcagc ggatatctgg gtgaccgccg
    11401 ccataggtat ggttattggc agcggtatgt acgaactggg tatttatggt tcagtgatga
    11461 ccttgttggt gctggaagtc ttccatcaat taaccttccg cctgatgaac aaaaattacc
    11521 atttacagct gacattagtg aacggtaata tggtatcaat gctcgactgg tttaagcagc
    11581 aaaaaataaa aactgattta gtttcgttgc aggaaaatga agatcatgaa gtggtcgcca
    11641 ttgatatcca gcttcatgcc acaacgtcga tagaagacct gttgcgatta ttaaaaggta
    11701 tggcaggggt gaaaggcgtt tcaattagct aatctgtgtg gcagcgtagc cagactcacc
    11761 gtaagcctga aattcacacc agataatcaa tatgctgatg gcgtaccagc tcattgatcc
    11821 gcttaagatt gaggatcatc atgatattgt gtttatggca gtagaacgtt tttagcgata
    11881 ttttcagttt ctccagaata tcttcagttg tcatttgatt cgcaatgcag ctcagaacct
    11941 gatgttgctt gcgcgaaagt gacacattcg cttcaggcac agagcagtaa ctgccattga
    12001 tgagtgcgac aaaatcaatg atagtgatag tccgcggaac aaaggtcacc ggtactttcc
    12061 ccagacagcg tttaatatta aagggggaga aaacaatgac atggacaggt tttaatcgtt
    12121 caatttgagt gagaaaatta gaatgaaata cattattcat taatgtatca ataatgacga
    12181 aggcattctg atgcaacatt acgtcgatct cttcttcgtt ctgtatatga acgacattac
    12241 ctttactcag aatgtttttc atcgcggtga agaacatcgt atccctggta attataagaa
    12301 acataaccga ctcctggtgc ggacattaac tttataatca atttgccaaa ggtaattaac
    12361 aggacagggc ttataataat aatagttagc ttactgtata ttaattttat gtaatacatg
    12421 atgcagatgc tgagaaagca tctgcatctt tcggtggtgt tatttgacca gctcaggtgt
    12481 tacctgactc accgcattgg tgtagtaagg cgcaccccag cccggttccg gccagaatgc
    12541 accatagccg taatcccaca ggttataggt ggtattaacc acttctctca aatgccacac
    12601 ctggatgccc tgcatattca cttccaggaa gttatacggg actttattga taaagccagg
    12661 ttgttcaccc tgaatggtac cgaggatggt aacaaagtga ttacgatagt tcactgggtc
    12721 aaggaagccg ctttgtctgg cgagcagtcg gccctgatag ttggcttcaa tatcaggttt
    12781 cgcatagcta tccaacggta atacagcgat ttctaacaac gtatccgttt tgccattgat
    12841 aacgttgata accttcccac caaagcgcgc ttgttgacca acatataacc ccggctggtt
    12901 atgaacagca acaaaacttt tttgaatatc aggttggtta ttgcctttga tattttgcgg
    12961 aattgaacta catgcggcaa gcaaaaatga aaggctgagg atgagtgcac
//
LOCUS       DAJVRF010000115        12933 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJVRF010000115 DAJVRF010000000
VERSION     DAJVRF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12933
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.115"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(566..1657)
                     /gene="ychF"
                     /locus_tag="OQN13_003012"
     CDS             complement(566..1657)
                     /gene="ychF"
                     /locus_tag="OQN13_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCS1616304.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1774..2358)
                     /gene="pth"
                     /locus_tag="OQN13_003013"
     CDS             complement(1774..2358)
                     /gene="pth"
                     /locus_tag="OQN13_003013"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCS1616305.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2635..2913
                     /gene="ychH"
                     /locus_tag="OQN13_003014"
     CDS             2635..2913
                     /gene="ychH"
                     /locus_tag="OQN13_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCS1616306.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2968..4647)
                     /gene="dauA"
                     /locus_tag="OQN13_003015"
     CDS             complement(2968..4647)
                     /gene="dauA"
                     /locus_tag="OQN13_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCS1616307.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4771..5718)
                     /gene="prs"
                     /locus_tag="OQN13_003016"
     CDS             complement(4771..5718)
                     /gene="prs"
                     /locus_tag="OQN13_003016"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCS1616308.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5869..6720)
                     /gene="ispE"
                     /locus_tag="OQN13_003017"
     CDS             complement(5869..6720)
                     /gene="ispE"
                     /locus_tag="OQN13_003017"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCS1616309.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6720..7343)
                     /gene="lolB"
                     /locus_tag="OQN13_003018"
     CDS             complement(6720..7343)
                     /gene="lolB"
                     /locus_tag="OQN13_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCS1616310.1"
                     /translation="MPQPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7557..8813
                     /gene="hemA"
                     /locus_tag="OQN13_003019"
     CDS             7557..8813
                     /gene="hemA"
                     /locus_tag="OQN13_003019"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCS1616311.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8855..9937
                     /gene="prfA"
                     /locus_tag="OQN13_003020"
     CDS             8855..9937
                     /gene="prfA"
                     /locus_tag="OQN13_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCS1616312.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9937..10770
                     /gene="prmC"
                     /locus_tag="OQN13_003021"
     CDS             9937..10770
                     /gene="prmC"
                     /locus_tag="OQN13_003021"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS1616313.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10767..11159
                     /locus_tag="OQN13_003022"
     CDS             10767..11159
                     /locus_tag="OQN13_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCS1616314.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11163..11971
                     /locus_tag="OQN13_003023"
                     /pseudo
     CDS             11163..11971
                     /locus_tag="OQN13_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
     gene            12007..12861
                     /gene="kdsA"
                     /locus_tag="OQN13_003024"
     CDS             12007..12861
                     /gene="kdsA"
                     /locus_tag="OQN13_003024"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCS1616315.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3245 a         3225 c         3343 g         3120 t
ORIGIN      
        1 aacagcggtt aaaaaatttg gtgaagattt tttgtaataa tcctaattag ttatgggaat
       61 ttcgcttgat gtatctgcat aacaaaaaca atatgcgcca tttttgcatt actaataaga
      121 aaaagcaaaa aacatcgata ttcatttttg gtcaatgggt tttctggcct atcgttgtta
      181 ttgtgctact ggttatcatt ttcatcttgc caggatcgca cgtaatcacg ttattcgggg
      241 actttatctg cgggatatta cccttcaggt aagtcctttt ctcgccagca tattggagcg
      301 tgatcaatat tgatcagctg tgaagggggt gatgtgtagt tgaacaaaaa cttacgttat
      361 atcactacta atgagatatc cgtcatctga cactgatgtc tttaattatt ctcgtggttc
      421 actgtaggca ataagcacaa aagtgtagga tgttacaaga atgattagga ctcggtgaaa
      481 tgaaaaatcc acgcaattgc gtggatttta tatacttttg cgctcttcat gagatttagc
      541 gaaacctcat gagacaataa attaattaga cgttgaaaag gaagttcatc acatcgccat
      601 ctttaacgat gtaatcttta ccttctgcac gcattttgcc tgcttctttc gcgccttgtt
      661 cacctttgta agtgatgaaa tcttcaaacg agatggtttg tgcacggata aagccttttt
      721 caaaatcagt atggattttg cccgctgcct gcggcgcggt tgctccaacc ggaatggtcc
      781 atgcacgcac ttctttcacc ccagcggtga agtaagtttg caggttcagc agcttataac
      841 cgacacggat cacacggttc aggcccggtt cttccagccc aagctcctgc ataaactcgt
      901 cacgttcttc gtcgtccagt tcggcaatgt ctgcttcaac agcagcacaa accggaacca
      961 caacagaacc ttctttcgcc gcgatttcac gcacctggtc aagatatggg ttgttttcaa
     1021 aaccgtcttc gttgacgttg gcgatgtaca ttgttggttt cagcgtcagg aagctcaggt
     1081 aacgaatagc cgctttctct tcagcgctta aatccagcgc gcgcagcata cctgcgtttt
     1141 ccagctgggg caggcatttt tctaggaccg ccagctcagc tttcgcgtct ttatcgccac
     1201 ctttggcttt cttctgtacg cgatgaatcg cacgttcgca ggtgtcgagg tctgccagcg
     1261 ccagttcggt gttgataact tcaatatcgt cagccgggtt aactttgccg gaaacgtgaa
     1321 tgatgttgtc attttcaaag cagcgaacaa cgtgaccgat cgcttcggtt tcacggatgt
     1381 tggtcaggaa ctggttaccc agaccttcgc ctttcgatgc gccttttacc agaccggcga
     1441 tatcgacaaa ttccatggtc gtgggaagcg tacgttgcgg ttttacgatt tcagccagtt
     1501 gatccaggcg agggtcaggc attggtacga cgcctgtgtt cggctcaatg gtgcagaatg
     1561 gaaagttggc cgcttcaata ccggctttgg tcagcgcgtt gaacagggta gatttcccga
     1621 cgttgggcaa accgacgata ccgcatttga atcccatgat ttaaatcacc ttaatatctt
     1681 aataatcaac ctgttattgc taacagattg cagaaatgga aataactttg cctattatac
     1741 acggcactcg gcaaaaatgc cgcagacaac gacttattgc gctttaaagg cgtgcaatcg
     1801 gttcgttgct ttggtcaagc catctgtaaa ccacatttca gtacaacgcg ccgcttcgtc
     1861 gatggcttca tcaattaact tctgttcact aacaggcggt ttgcctaaca caaaaccgac
     1921 aactttattt ttatcgccag gatgaccgat tccgatgcgt aaacggtgaa agttagggtt
     1981 attacccaat ttactgatga tgtctttcag tccattgtga ccaccatggc caccgcccaa
     2041 tttaaatttg gcgacgccag gaggcagatc cagttcgtcg tgggccacca gaatttcgtc
     2101 cggattaatg cggaaaaaac tggccatcgc cgcaacggct ttgccgctga gattcataaa
     2161 tgtagtcggg actaacaggc ggacatcttc gcctccaaga gtgactcgcg aagtataacc
     2221 aaagaattta gcctcttcgc gcagcggagc gcgcaaacgc tctgccagta agtcaacgaa
     2281 ccaggcacca gcattatgtc gcgttgcggc gtattcagca ccggggttcg ccaggccgac
     2341 aatcaattta atcgtcacgt tttttgtcct gagtgtgtac ataactggcg cgtagtttac
     2401 tggttgcggc cccgcttgac aaaaaactgc gtatcaaatg cagataacgt aataattgcc
     2461 tgagtggact attagaaagt caaggtgttc aggcgtttat ttgtaaagtt ttgttgaaat
     2521 aagggttgta attgtgatca cgcccgcaca taacccacag ggtgttgtct atactttaca
     2581 cataaggaag aggggtattc cctgttacaa cccagaaagt tccggaggtg acatatgaaa
     2641 cgcaaaaacg cttcgttact cggtaacgtg ctcatggggt tggggctggt ggtaatggtg
     2701 gtcggcgtgg ggtattcaat cctcaaccag ttaccacagt ttaatatgcc ccagtatttc
     2761 gcacatggtg cagtgctaag tattttcgtc ggtgccattc tctggctggc gggtgcccgt
     2821 gttggcgggc atgaacaggt gtgcgaccgt tactggtggg ttcgtcacta tgacaaacgt
     2881 tgccgccgta gcgataatcg ccgtcatagc taacaaataa tgcagtttgc tgaccagtca
     2941 gtttgcgctg actggtcaat ctcgtactta taaatccgcc atcgccgcgc gacgattcgg
     3001 gaagaacgcg aggcgtcccg ggatcggttg aatgcctgcg cgagccatag tgcgcagtgg
     3061 ctggaattcc acgttgcaca cgcgcagttc acatccttcg ggcagacgct tcacaaaacg
     3121 ctggaacgca tcaagaccac cagcatcaag taccggaacg gcatcccact tcagaatcac
     3181 aatccgtttg ccttcaagac gtgactccag gtccgtgaac aagccttcag cagcagcaaa
     3241 aaacagcggg ccaataacgc gcagtaccag aacatcgtct ggaacatcta cgactaccgg
     3301 tgccaggcga gtcatacgtg cgatacgacg cataaacagc agcgatgcca gcacgatccc
     3361 cacgctgatg gcaataacca tatcaaacag cacggtcagc gacatgcaca gcagcatgac
     3421 aatgatgtca tctttcggtg catgacgcag caagtcgacc actttatgcg cttcactcat
     3481 gttccacgcc accatcaaca gcagggctgc catagcggaa agcggcagcc aggagagcag
     3541 cggtgccagt accagcaggg caagaataac cagaatagag tggatcaccg ccgagatagg
     3601 ggaagttgcc ccggcacgga cgttagcggc agaacgcgcg atggcagctg tagcggtaat
     3661 accaccaaag aacggagcga taatattccc cagtccctgt ccaaccagtt cgctgttcgc
     3721 cttgtgtttc gtcccggtca taccatccag taccacggcg cagagcagag attcgattgc
     3781 gccgagcatt gccattgaga atgccgcagg cagcagtgtg cgaatagaat cccaggttag
     3841 cgtgaattct gaattaggca gatcccacgg cagcaccagt tgcggcagca gttgcggaat
     3901 accgttaccc tgagaaccat cggccaggac gtagtggaat tgcgaaccga tggtagcaac
     3961 atgtccgccg agcaggttaa caatccccat caccgcgcaa ccagccagca aggccggaag
     4021 gtgaccgggt aaacgaatgc ccagacgcgg ccaaaaaaca agaataccta gcgtcacaat
     4081 gccaatggca gcatcaccca cattaatggt cggcagcgcc ataaataatg cgccgacttt
     4141 ttgtagataa tgttccggga catgggccat ttgcagaccg agaaaatctt taatctgcat
     4201 ggtaccgatg gtgatcccga tacccgaggt gaaacctaag gtgacggaaa ccggaatata
     4261 ctcaatcagg cgaccaaagc gtgccagacc catcagaatc aaaaagatcc ccgacagcaa
     4321 ggtcgcaacc agcagtcctg ccagtccaaa ctgttgcgac acgggataga gaattaccac
     4381 aaatgccgca gtcggaccgg aaacgctaaa gcgtgaccca cccgtcagag caatgacaat
     4441 ccccgcaaca gctgcggtat ataaaccgta ctggggtgcc acaccactac caatagccaa
     4501 cgccatcgcc agcgggatag caataatccc gacggttatc ccggcaatca ggtcacgggt
     4561 aaaccgtgcg gcagtatatt tttctttcca gcaagcgtcg atcagagcgc ggaaaggcat
     4621 cacatgtgag gaaaatattt tgttcacaat aatgtttcat ccgtgagcgc atcatctgtc
     4681 aactaaatgg caggtgaagg aggcataggt catacaaatg gatattacag acaaaaaaac
     4741 ccgccgcagc gggtctttga gcgggttcga ttagtgttcg aacatggcag agatcgattc
     4801 ttcgttgctg atacgacgaa tcgcttcggc cagcatacct gacagggtca gagtacgcac
     4861 gttcggcagt gatttgattt catcgctcag cggaatggta tcgcagacaa cgacttcatc
     4921 aattacagag ttacgcaggt tgttcgccgc gttgccagag aagatcgggt gagtcgcgta
     4981 cgcaaataca cgtttagcac cacgttcttt cagagcttca gcagctttac acagcgtacc
     5041 gccagtgtcg atcatatcat cgaccagtac gcagtcacga cctgcaacgt caccgatgat
     5101 atgcatcacc tgggaaacgt tcgcacgcgg acgacgtttg tcgatgattg ccatatcggt
     5161 atcgttcagc agcttagcga tagcgcgggc acgcacaacg ccgccgatgt ccggagaaac
     5221 cacaattggg ttatccagat tcagctgcag catgtcttcc agcaggatcg ggctaccaaa
     5281 tacgttatca accggaacgt cgaagaaacc ctgaatctgt tcagcgtgca gatccactgt
     5341 cagcacacgg tcaacaccga cgctggagag gaagtctgca accactttcg cagtgattgg
     5401 tacacgagcg gaacggacgc gacggtcctg gcgcgcatag ccaaagtagg ggataacagc
     5461 ggtgatacga cctgcggaag cacgacgcag ggcatcaacc ataacgacta attccatcag
     5521 gttgtcgtta gtaggggcac aagtggactg gatgatgaaa atatcaccac cgcgtacatt
     5581 ttcgttgatt tgtacactga cttcgccgtc gctaaagcga cctacagcgg cgtcgccgag
     5641 tgaagtgtac aggcggttgg caatacgttg tgctagttcc ggggtggcgt taccagcaaa
     5701 aagcttcata tcaggcacga gaagaacctc aggcatgcgt ccattggtgg aaagaatctg
     5761 ccgaaaactg tgcgggccag gcatgatcct ttccaggcgg tgtattaaag agcgcgatgc
     5821 aacgtctgga acaaggtgac gttgtcaccg aaactcagct tgcccggctt aaagcatggc
     5881 tctgtgcaat ggggaaagat taacaccttt cgccacaaag ccattgagcc attccggggc
     5941 ttgctctagc acctggcggg cttcagactc tgtatcaaat tcagcaaaga cacaggcccc
     6001 tgtcccagtc aggcgcgacg gggcgtattc taacagccag gaaagcaccg catcaacctc
     6061 gcgaaaacgt tttcttgcga taacctcgca atcattgctg aattcacatt ttagcaacgt
     6121 ttctattgac ctttttggcg tattgcgcgg gagttcagga tctttaaaaa tcactggtgt
     6181 cggaatactt acaccagggt gcgccaccag ataccacttc tctggcggat ccaccggcgt
     6241 tagtatttca ccaacgcctt cggcaaacgc ggcatgcccc cgaacaaaga caggaacatc
     6301 tgcgcccagc gtcagcccca tttccgccag ctcatccatg cttagcccgc attgccagag
     6361 atgatttaat gccaccagga ccgtcgcggc attggatgaa ccaccgccga gaccgccgcc
     6421 catcggcaaa cgcttgtcaa tgctgatatt cgcaccgctt cccgtcggaa gacgcccgct
     6481 gtctgccgca gttttcatca acaaccgcgc tgcgcgaacg atcaggttat cttcatgttc
     6541 cacgccttca acgggcgtta acagacgaat atccccatcg tcacgaagct caatgctgat
     6601 ggtgtcgccg taatcaagaa actgaaacag cgtttgcagc gtgtggtaac catccgcacg
     6661 ctgaccggta atgtataaaa acagattaag ttttgccgga gagggccact gtgtccgcat
     6721 tatttcacta tccagttatc catttttaac ttgatgcgtt gaccaccgtc ggtgagttcc
     6781 atattggctg gcatcgcagg ttgcgttttg gtgtcataac caccataaac aaccttccag
     6841 tttttgccat tctggctgta ggtaatttcg ctcaggcggt actggtcgtc cagtttgtag
     6901 tcggttgcat cacccggtaa acctaaaatc cattggcgca agctgttgag cggaattggc
     6961 attccggtca atttgccaat catctcttcg gcgtcatcgg cggtataacg ctgaccttta
     7021 ttgtcgacta actgcacgtt acccggttga gcattcagct ccagttccgt gctgcccaat
     7081 gggttagtga gcagcagacg gtagcgatcc tggccggttt gctgccagaa aaagcgggcg
     7141 tacacttttt gttggtcaga aatataagcg aacgcgccgc gagtctgata ctgattaaga
     7201 ttgcgcacgt cttgctgatg ctgacgccat tgtggcgaat ccgggctttt gccaggacct
     7261 ttgggcgtgg taacggaaca ggcagtgagc acaagagcag ccagcggtag caggcggata
     7321 agacgaaaat cgggctgggg catagtgatg acaagtcctt gagatacgtt gcagttataa
     7381 cccttaatgc tagcgttacc gtccgctatc gtctatgttc aagttgtctt aattgccaga
     7441 atctaacggc tttcggcaat tactccaaaa gggggcgctc tcttttattg atcttacgca
     7501 tcctgtatga tgcaagcaga ctaaccatat caacgttggt attatttccc gcagacatga
     7561 cccttttagc actcggtatc aaccataaaa cggcacctgt atcgctgcga gaacgtgtat
     7621 cgttttcgcc ggataagctc gatcaggcgc ttgacagcct gcttgcgcag ccgatggtgc
     7681 agggcggcgt ggtgctgtcg acgtgcaacc gcacggaact ttatcttagc gttgaagagc
     7741 aggataacct gcaagaggcg ttaatccgct ggctttgcga ttatcacaat cttaatgaag
     7801 aagatctgcg taaaagcctc tactggcatc aggataacga cgcggttagc catttaatgc
     7861 gtgttgccag cggcctggat tcactggttc ttggggagcc gcagatcctc ggtcaggtta
     7921 aaaaagcgtt tgccgattcg caaaaaggtc atatgaaggc cagcgaactg gaacgcatgt
     7981 tccagaaatc tttctctgta gcgaaacgcg ttcgcactga aacagatatc ggtgccagtg
     8041 ctgtgtctgt cgcttttgcg gcttgtacgc tggcgcggca gatctttgaa tcgctctcta
     8101 cggtcacagt gttgctggta ggcgcgggcg aaaccatcga gctggtagcg cgtcatctgc
     8161 gcgaacataa agtacagaag atgattatcg ccaaccgcac tcgcgaacgt gcccaaattc
     8221 tggcagatga agttggcgcg gaagtgattg ccctgagtga gatcgacgaa cgtctgcgcg
     8281 aagccgatat catcatcagt tccaccgcca gcccgttacc gattatcggg aaaggcatgg
     8341 tggagcgcgc attaaaaagt cgtcgtaacc aaccaatgct gttggtggat attgccgttc
     8401 cgcgcgatgt tgagccggaa gttggcaaac tggcgaatgc ttatctttat agtgtggacg
     8461 atctgcaaag catcatttcg cacaacctgg cgcagcgtaa agccgcagcg gttgaggcgg
     8521 aaactattgt cgctcaggaa accagcgaat ttatggcgtg gctgcgagca caaagcgcca
     8581 gcgaaaccat tcgcgagtat cgcagccagg cagagcacgt tcgcgatgag ttaaccgcca
     8641 aagcgttagc ggcccttgag cagggcggcg acgcgcaagc cattatgcag gatctggcat
     8701 ggaaactgac taaccgcttg atccatgcgc caacgaaatc acttcaacag gccgcccgtg
     8761 acggggataa cgaacgcctg aatattctgc gcgacagcct cgggctggag tagcagtaca
     8821 tcattttctt tttttacagg gtgcatttac gcctatgaag ccttctatcg ttgccaaact
     8881 ggaagccctg catgaacgcc atgaagaagt tcaggcgttg ctgggtgacg cgcaaactat
     8941 cgccgaccag gaacgttttc gcgcattatc acgcgaatat gcgcagttaa gtgatgtttc
     9001 gcgctgtttt accgactggc aacaggttca ggaagatatc gaaaccgcac agatgatgct
     9061 cgatgatcct gaaatgcgtg agatggcgca ggatgaactg cgcgaagcta aagaaaaaag
     9121 cgagcaactg gaacagcaat tacaggttct gttactgcca aaagatcctg atgacgaacg
     9181 taacgccttc ctcgaagtcc gagccggaac cggcggcgac gaagcggcgc tgttcgcggg
     9241 cgatctgttc cgtatgtaca gccgttatgc cgaagcccgc cgctggcggg tagaaatcat
     9301 gagcgccagc gagggtgaac atggtggtta taaagagatc atcgccaaaa ttagcggtga
     9361 tggtgtgtat ggtcgtctga aatttgaatc cggcggtcat cgcgtgcagc gtgttcctgc
     9421 tacggaatcg cagggtcgta ttcatacttc tgcttgtacc gttgcggtaa tgccagaact
     9481 gcctgacgca gaactgccgg acatcaaccc agcagattta cgcattgata ctttccgctc
     9541 gtcaggggcg ggtggtcagc acgttaacac caccgattcg gcaattcgta ttactcactt
     9601 gccgaccggg attgttgttg aatgtcagga cgaacgttca caacataaaa acaaagctaa
     9661 agcactttct gtacttggtg ctcgtatcca cgctgcagaa atggcaaaac ggcaacaggc
     9721 cgaagcgtct acccgtcgta acctgctggg gagtggcgat cgcagcgacc gtaaccgtac
     9781 ttacaacttc ccgcaggggc gcgttaccga tcaccgcatc aacctgacgc tctaccgtct
     9841 ggatgaagtg atggaaggta agctggatat gctgattgaa ccgattatcc aggaacatca
     9901 ggccgaccaa ctggcggcgt tgtccgagca ggaataatgg aatatcaata ctggttacgt
     9961 gaagcaataa gccaacttca ggcgagcgaa agcccacgcc gtgacgctga aatcttattg
    10021 gagtatgtca ctggcagggg gcggactttt attctggcct ttggtgaaac gcagctgact
    10081 gacgaacaat gtcagcaact tgatgcgcta ctgacacgtc gtcgcgatgg tgaacccatt
    10141 gctcatttaa ccggggtgcg agaattctgg tcgttgccgt tatttgtttc gccagcgacc
    10201 ttaattccgc gcccggatac ggagtgtctg gtggagcagg cactggcgcg gttgcctgaa
    10261 caaccttgcc gtattctcga tctcgggacg ggtaccgggg cgattgcgct tgcgctggct
    10321 agcgagcgcc cggactgcga aattatcgct gtagatcgta tgcctgatgc tgtctccctg
    10381 gcacaacgta atgcccagca tctggcgatc aaaaatatcc acattctgca aagcgactgg
    10441 tttagcgcgc tagccgggca gcagtttgcg atgattgtca gcaatccgcc gtatattgac
    10501 gagcaggacc cacatcttca acaaggcgat gtccgctttg agccgctcac tgcgctggtt
    10561 gcggcagaca gtggaatggc agacatcgtg catatcatcg aacagtcgcg taacgcgctg
    10621 gtatccggcg gctttctgct tctggaacat ggctggcagc agggcgaagc ggtgcgacaa
    10681 gcatttatcc tcgcggggta tcatgacgtc gaaacctgcc gtgactatgg tgataacgag
    10741 cgcgtaacgc tcggccgcta ttatcaatga caagtttttc tacactgctt agtgttcatc
    10801 ttattagtat cgcgctttct gttgggctat taaccttacg tttctggcta cgttatcaga
    10861 agcatccaca ggcatttgct cgctggacgc gcattgtgcc gccggttgtc gatacggtgt
    10921 tattgttaag cggcattgcg ttgatggcta aagcgcacat cctgccattt tccgggcagg
    10981 cacagtggct gactgaaaag ctgtttggag ttatcattta tatcgttttg ggttttattg
    11041 cactcgatta tcgtcgtatg cacagtcagc aggcgcgcat tattgccttc ccgctggcgt
    11101 tggtggtgct gtacatcatc attaaactcg ccaccacaaa agtaccgtta ctggggtaag
    11161 tcatgagatc gttagctgat ttcgaattta ataaagcgcc attgtgcgaa ggcatgatcc
    11221 tggcttgcga agcaatccgc cgcgattttc cctcgcaaga tgtttacgac gaactggagc
    11281 gtctcgttag tctggcgaag gaagaaatca gccagcttct gcctttagaa gagcagctgg
    11341 aaaaactgat cgcgctgttt tacggcgact ggggatttaa agcctcacgc ggcgtttatc
    11401 gtctttccga tgcattatgg ctggaccagg tgttaaagaa tcgacaggca gtgcggtatc
    11461 attaggtgcg gttttattat gggtcgcgaa tcgtctcgat ttgccgctgc tgccggtgat
    11521 tttccctacg cagctgatat tgcgcattga atgtccggat ggcgaaattt ggctgattaa
    11581 tccttttaac ggtgaatcgt taagcgaaca tatgctggac gtatggttaa agggaaatat
    11641 cagcccgtcg gcggaactgt tttatgaaga ccttgatgaa gctgataaca ttgaggtaat
    11701 ccgcaaattg ttggatacac tcaaagcctc gttgatggaa gaaaatcaga tggagctggc
    11761 gttacgcacc agcgaagctt tattacaatt caacccggaa gatccctatg aaattcgcga
    11821 tcgcgggttg atttatgcgc aactggattg cgaacacgtt gcgttgaacg atttaagtta
    11881 tttcgttgaa cagtgtccgg aagacccgat cagcgaaatg atccgtgcgc aaataaataa
    11941 catcgcgcat aaacatattg tgctgcatta attaatcgac attttactca agattaaggc
    12001 gatcctatga aacaaaaagt ggttagcatt ggcgacatca acgtagcaaa tgacctgccg
    12061 ttcgtactgt ttggtggtat gaacgtgctg gaatctcgcg atctggcgat gcgcatttgc
    12121 gagcactacg taaccgtgac ccagaaactg ggtatccctt acgtgttcaa agcctctttt
    12181 gacaaagcca accgctcctc catccactct tatcgtggac cgggcctgga agaagggatg
    12241 aaaatcttcc aggagttgaa gcagactttt ggcgtgaaaa ttatcaccga cgttcacgaa
    12301 ccaagtcagg cacagcccgt tgctgatgtc gtggatgtga ttcagttgcc ggcgtttctt
    12361 gctcgccaga ctgacctggt tgaagccatg gcgaaaaccg gtgcggtaat taacgtcaag
    12421 aaaccacagt ttgtcagccc gggacagatg ggtaatatcg ttgataaatt caaagaaggc
    12481 ggcaacgaaa aagtgattct ttgcgatcgc ggtgctaact tcggctatga caacctggtt
    12541 gtcgatatgc tgggcttcag cattatgaag aaagtgtctg gtaactcgcc ggtgattttc
    12601 gacgtgaccc acgcactgca atgccgcgat ccgtttggcg cagcttccgg tggtcgtcgt
    12661 gctcaggtgg ctgagctggc acgagccggt atggcggtag gtctggcggg gctgtttatt
    12721 gaagcgcatc cggatccgga acatgcgaaa tgtgatggtc catccgcgct gccgctggct
    12781 aaactggaac cgttcctcaa gcagatgaaa gcgattgatg atctggtgaa aggtttcgaa
    12841 gaactggata ccagcaagta atcttttttg cttgaaaaat aaagccttag cgttctgcgt
    12901 taagactttt ttcatgggtg ccagatacaa aaa
//
LOCUS       DAJVRF010000116        12835 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJVRF010000116 DAJVRF010000000
VERSION     DAJVRF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12835
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.116"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            28..1230
                     /locus_tag="OQN13_003025"
     CDS             28..1230
                     /locus_tag="OQN13_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCS1616316.1"
                     /translation="MNLSANAQATLLLTSDFSRAAASEYKPLSNSEWGKFALWLKHQR
                     ISPAELLVPQPQEKLTGWSDPRISQERILGLLARGHSLALAVDKWQRAGLWILTRGDA
                     DYPVRLKNRLRTDAPPVLFGCGNKALLQAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQ
                     GICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLIAGNLVLISP
                     FYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQ
                     DMQSANSRLVENGASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYA
                     VMPAYTPVDFYQLFVAELAILAKESISIERLASCTGLTIEQISVWLNRAEEEGRVIRL
                     GEGHYQFR"
     gene            complement(1276..1869)
                     /gene="yhgN"
                     /locus_tag="OQN13_003026"
     CDS             complement(1276..1869)
                     /gene="yhgN"
                     /locus_tag="OQN13_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCS1616317.1"
                     /translation="MNAIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2061..3164
                     /gene="asd"
                     /locus_tag="OQN13_003027"
     CDS             2061..3164
                     /gene="asd"
                     /locus_tag="OQN13_003027"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS1616318.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3437..5623
                     /gene="glgB"
                     /locus_tag="OQN13_003028"
     CDS             3437..5623
                     /gene="glgB"
                     /locus_tag="OQN13_003028"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCS1616319.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5620..7593
                     /gene="glgX"
                     /locus_tag="OQN13_003029"
     CDS             5620..7593
                     /gene="glgX"
                     /locus_tag="OQN13_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCS1616320.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7611..8906
                     /gene="glgC"
                     /locus_tag="OQN13_003030"
     CDS             7611..8906
                     /gene="glgC"
                     /locus_tag="OQN13_003030"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCS1616321.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            8906..10339
                     /gene="glgA"
                     /locus_tag="OQN13_003031"
     CDS             8906..10339
                     /gene="glgA"
                     /locus_tag="OQN13_003031"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCS1616322.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10358..12805
                     /gene="glgP"
                     /locus_tag="OQN13_003032"
     CDS             10358..12805
                     /gene="glgP"
                     /locus_tag="OQN13_003032"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCS1616323.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEMR
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3014 a         3184 c         3533 g         3104 t
ORIGIN      
        1 ccttgcttct acctcggtaa aaaattgatg aatctttcag ccaatgcaca agcaactctc
       61 ctgctaacca gcgatttttc tcgcgcggcg gcgagtgagt ataaacctct tagtaatagt
      121 gaatggggga agtttgcatt atggctgaag caccaacgta tcagtcccgc cgagcttctg
      181 gtgccgcaac cgcaagagaa acttacaggc tggagcgatc cgcgtatttc tcaggagcgt
      241 attcttggct tgctggcgcg tggtcatagt ctggcgttgg cggtagataa gtggcaacgc
      301 gccggtttat ggatcttaac ccgcggagat gctgattatc ccgttcgctt gaaaaaccga
      361 ttgcgaacgg atgcacctcc cgttttattt ggctgcggga ataaagcatt actgcaagcg
      421 gaaggtatgg cgattgttgg ctcgcgagat gctccgactg acgatttgcg ctatacccaa
      481 caactggccg cgaaactggc ccaacagggg atttgcgtta tctctggtgg tgcgcgaggt
      541 attgatgaat gtgcaatggc gtcggcactg gaggccgggg gaactgccgt tggcgtatta
      601 gctgatagct tgttaaaaac gagtacgtta gtgaaatggc gtgaagggct tatagcaggc
      661 aacctggtgt tgatttcgcc gttttaccca gaggtacgtt tcaccgtcgg caatgcgatg
      721 gcgcgaaata aatatattta ttgccttgct gaaagcgcaa tggttgtacg tgcgggaatg
      781 accggtggaa cgataaccgg ggcgatggag gcattaaaac atcagtggct gcctgtgcag
      841 gttaaaccaa atcaggatat gcaatcagcc aattcacgat tagtagaaaa tggggcgtca
      901 tggagtgctg aacaggctga gaatgtgacg atcagactgc cagacgttcc tgggctgatg
      961 tatgacagag cactccgtaa cgctcaacca gaactgtttt cgctgcatga agatgacgca
     1021 aattacgcag taatgcccgc gtatacgcct gtcgattttt atcaactctt tgtggcggaa
     1081 ctggcgatcc ttgcaaagga atcgataagt attgaaaggc tggcgtcttg tactggttta
     1141 accatcgaac aaattagtgt gtggctgaac cgcgcagaag aagagggaag ggttatccga
     1201 ttgggcgaag gtcattatca gttcaggtaa taaaacaggt gccgcgtctg gttgtgtgca
     1261 gcacctgttt gaaccttacc ccttcatcca cattcgaatg ccgtcgagga acatctgggt
     1321 tgccatcatc accagaatca atcccatcag gcgttcaagt gcgttcaccc ctttctcgcc
     1381 cagcagacgt aaaaatagcg aagactgtag caggatgaca aaggtgccgc cccaggccag
     1441 cagcagagca atcaccagat gtcccatctg attcgggtac tgatgagaca acaacatcag
     1501 cgtggcgaga atagtcggcc cggcgactaa cggaattgcc aacggcacga taaatggctc
     1561 ttcacctgcc ggaagcccgc tgctatttcc tgaagcgctg gggaaaatca tcttaatggc
     1621 gattagaaac agaatgatgc cgccagaaat ggagacggtt tctgctcgca ggctgagaaa
     1681 tgccagaatt ttctcacccg caaacaggaa caccagcatc accaggagag caataagcaa
     1741 ctctcgcacc atgattgccc gccgtctttt cggttcagta tgtttcagta cggacatgaa
     1801 aataggcagg tttccgagcg gatccataat caggatcaat aaaactgctg cagaaatgat
     1861 tgcattcata actcaaattc cctgataatt gccgcggact ttctgcgtgc taacaaagca
     1921 ggataagtcg cattactgat ggcttcgcta tcattgatta atttcacttg cgactttggc
     1981 tgctttttgt atggtgaaag atgtgccaag aggagaccgg cacatttata cagcacacat
     2041 ctttgcagga aaaaacgctt atgaaaaatg ttggttttat cggctggcgc ggtatggtcg
     2101 gctccgttct catgcaacgc atggttgaag agcgcgactt cgacgccatt cgccctgtct
     2161 tcttttctac ttctcagctt ggccaggctg cgccgtcttt tggcggaacc actggcacac
     2221 ttcaggatgc ctttgatctg gaggcgctaa aggccctcga tatcattgtg acctgtcagg
     2281 gcggcgatta taccaacgaa atctatccaa agcttcgtga aagcggatgg caaggttact
     2341 ggattgacgc agcatcgtct ctgcgcatga aagatgacgc catcatcatt cttgaccccg
     2401 tcaatcagga cgtcattacc gacggattaa ataatggcat caggactttt gttggcggta
     2461 actgtaccgt aagcctgatg ttgatgtcgt tgggtggttt attcgccaat gatcttgttg
     2521 attgggtgtc cgttgcaacc taccaggccg cttccggcgg tggtgcgcga catatgcgtg
     2581 agttattaac ccagatgggc catctgtatg gccatgtggc agatgaactc gcgaccccgt
     2641 cctctgctat tctcgatatc gaacgcaaag tcacaacctt aacccgtagc ggtgagctgc
     2701 cggtggataa ctttggcgtg ccgctggcgg gtagcctgat tccgtggatc gacaaacagc
     2761 tcgataacgg tcagagccgc gaagagtgga aagggcaggc ggaaaccaac aagatcctca
     2821 acacatcttc cgtaattccg gtagatggtt tatgtgtgcg tgtcggggca ttgcgctgcc
     2881 acagccaggc attcactatt aaattgaaaa aagatgtgtc tattccgacc gtcgaagaac
     2941 tgctggctgc gcacaatccg tgggcgaaag tcgttccgaa cgatcgggaa atcactatgc
     3001 gtgagctaac cccagctgcc gttaccggca cgctgaccac gccggtaggc cgcctgcgta
     3061 agctgaatat gggaccagag ttcctgtcag cctttaccgt gggcgaccag ctgctgtggg
     3121 gggccgcgga gccgctgcgt cggatgcttc gtcaactggc gtaatcttta ttcattaaat
     3181 ctggggcgcg atgccgcccc tgttagtgcg taatacagga gtaagcgcag atgtttcatg
     3241 atttaccggg agttaaatag agcattggct attctttaag ggtggctgaa tacatgagta
     3301 ttcacagcct tacctgaagt gaggacgacg cagagaggat gcacagagtg ctgcgccgtt
     3361 caggtcaaaa aaatgtcaca accagaagtc aaaaatccaa ttggatgggg tgacacaata
     3421 aaacaggaag acaagcatgt ccgatcgtat cgatagagac gtgattaacg cgctaattgc
     3481 aggccatttt gcggatcctt tttccgtact gggaatgcat aaaaccaccg cgggactgga
     3541 agtccgtgcc cttttacccg acgctaccga tgtgtgggtg attgaaccga aaaccgggcg
     3601 caaactcgca aaactggagt gtctcgactc acggggattc tttagcggcg tcattccgcg
     3661 acgtaagaat tttttccgct atcagttggc tgttgtctgg catggtcagc aaaacctgat
     3721 tgatgatcct taccgttttg gtccgctaat ccaggaaatg gatgcctggc tattatctga
     3781 aggtactcac ctgcgcccgt atgaaacctt aggcgcgcat gcagatacta tggatggcgt
     3841 cacaggtacg cgtttttctg tctgggctcc aaacgcccgt cgggtctcgg tggttgggca
     3901 attcaactac tgggacggtc gccgtcaccc gatgcgcctg cgtaaagaga gcggcatttg
     3961 ggaactgttt atccctgggg cgcataacgg tcagctctat aaatacgaga tgattgatgc
     4021 caatggcaac ttgcgtctga agtccgaccc ttatgccttc gaagcgcaaa tgcgcccgga
     4081 aaccgcgtct cttatttgcg ggctgccgga aaaggttgta cagactgaag agcgcaaaaa
     4141 agcgaatcag tttgatgcgc caatctctat ttatgaagtt cacctgggct cctggcgtcg
     4201 ccacaccgat aacaatttct ggttgagcta ccgcgagctg gccgatcaac tggtgcctta
     4261 tgctaaatgg atgggcttta cccacctcga actactgccc attaacgagc atcccttcga
     4321 tggcagttgg ggttatcagc caaccggcct gtatgcacca acccgccgtt ttggtactcg
     4381 cgacgacttc cgttatttca ttgatgccgc acacgcagcg ggtctgaacg tgattctcga
     4441 ctgggtgcca ggccacttcc cgaccgatga ctttgcgctt gccgaatttg atggcacgaa
     4501 cttgtatgaa cacagcgatc cgcgcgaagg ctatcatcag gactggaata cgctgatcta
     4561 caactatggt cgccgtgaag tcagtaactt cctcgtcggt aacgcgcttt actggattga
     4621 acgttttggt attgatgcgc tgcgcgtcga tgcggtggcg tcaatgattt atcgcgacta
     4681 cagccgtaaa gagggggagt ggatcccgaa cgaatttggc gggcgcgaga atcttgaagc
     4741 gattgaattc ttgcgtaata ccaaccgtat tcttggtgag caggtttccg gtgcggtgac
     4801 aatggctgag gagtctaccg atttccctgg cgtttctcgt ccgcaggata tgggcggtct
     4861 gggcttctgg tacaagtgga acctcggctg gatgcatgac accctggact acatgaagct
     4921 cgacccggtt tatcgtcagt atcatcacga taaactcacc ttcgggattc tctacaacta
     4981 cactgaaaac ttcgtcctgc cgttgtcgca tgatgaagtg gtccacggta aaaaatcgat
     5041 tctcgaccgc atgccgggcg acgcatggca gaaattcgct aacctgcgcg cctactatgg
     5101 ctggatgtgg gcattcccgg gcaagaaact gctgttcatg gggaacgaat ttgcccaagg
     5161 ccgtgagtgg aaccatgacg ccagcctcga ctggcatctg ttggaaggcg gcgataactg
     5221 gcaccacggt gtccagcgtc tggtgcgcga tctgaacctc acctaccgcc accataaagc
     5281 aatgcatgaa ctggattttg acccgtacgg ctttgaatgg ctagtggtgg atgacaaaga
     5341 acgctcggtg ctgatctttg tgcgtcgcga taaagagggt aacgaaatta tcgttgccag
     5401 taactttacg ccggtgccgc gtcatgatta tcgcttcggc attaatcagc caggcaaatg
     5461 gcgtgaaatc ctcaataccg attccatgca ctatcacggc agtaatgcag gcaatggcgg
     5521 cacggtacac agcgatgaga ttgccagcca cggtcgtcag cattcactaa gcctgacact
     5581 accaccgctg gccactatct ggctggttcg ggaggcagaa tgacacgact cgccattggc
     5641 aaacccgctc ccctcggcgc gcattacgac ggtcagggcg tcaacttcac acttttctcc
     5701 gctcatgccg agcgggtaga gctgtgtgtc tttgacgcca atggccagga acatcgctat
     5761 gacttgccag ggcacagtgg cgacatttgg cacggttatc tgccggatgc gcgcccgggt
     5821 ttgcgttatg gttatcgcgt tcatggcccc tggcaacccg ccgaggggca tcgctttaac
     5881 ccggcgaagt tgttgattga tccttgcgcg cggcaaattg aaggggaatt caaagataac
     5941 ccgctgctgc acgccggtca taatgaacct gactatcgcg acaacgccgc cattgcgccg
     6001 aaatgcgtag tggtggttga tcactatgac tgggaagatg atgccccgcc gcgcacgccg
     6061 tggggcagca ccatcattta tgaagcccat gttaaaggat taacgtacct gcacccggag
     6121 atcccggtcg agatccgtgg cacttataaa gccctcgggc atccggtgat gatcaactat
     6181 ttgaaacagt tgggcattac cgcgctggaa ctgctgccgg tggcgcagtt tgccagtgaa
     6241 ccacgtctgc aacgcatggg gctaagtaac tactggggtt acaacccggt ggcgatgttt
     6301 gcgctgcatc cggcgtatgc ctgctcgcca gaaacggcgc tgcatgagtt tcgcgatgca
     6361 atcaaagcac tgcataaagc gggtatcgaa gtcattcttg atatcgtgct caaccatagt
     6421 gcggaactgg acctcgacgg cccgttattc tcgctgcgtg ggatcgacaa ccgtagctat
     6481 tattggataa gagaagacgg cgattatcac aactggaccg gttgtggcaa cacgctcaat
     6541 ttgagtcatc cggcggtggt ggattatgcc tgcgcctgcc tgcgttattg ggtagaaacc
     6601 tgtcacgtcg atggtttccg ctttgatctg gcggcagtca tgggccgtac gccagagttc
     6661 cgtcaggatg cgccgttgtt taccgctatc cagaactgcc cggtgctctc gcaggtgaag
     6721 ttaattgctg aaccgtggga tatcgccctt ggtggttatc aggtgggaaa tttcccgccg
     6781 ctgtttgccg agtggaacga tcatttccgc gatgctgccc gtcgtttctg gctacattat
     6841 gatttgcctc tgggggcgtt tgccgggcgt tttgctgcct ccagcgatgt ttttaaacgt
     6901 aatggtcgtc tgccgagtgc cgcgattaat ctcgtcactg cgcatgacgg ttttacgctt
     6961 cgcgactgcg tttgcttcaa ccataaacac aatgaagcaa acggagaaga aaatcgcgac
     7021 gggaccaaca acaattacag taacaatcat ggtaaagaag ggttaggcgg ttctcttgac
     7081 ctggttgaac ggcggcgcga cagtattcac gccctgttaa caacgttgtt gctctcccag
     7141 ggtacgccga tgttactggc cggtgacgaa cacggtcaca gccagcatgg caataacaat
     7201 gcctactgtc aggataacca attaacctgg ttggactggt cgcaggcaag cagtggttta
     7261 accgcattta ccgccgcgtt aatccatctg cgcaagcgca ttcccgcttt ggtggagaat
     7321 cgctggtggg aagaaggcga cggcaatgtc cgttggctaa atcgatatgc tcaaccttta
     7381 agcacggatg agtggcaaaa cgggccgaaa cagctgcaaa ttctgctctc ggatcgcttt
     7441 ttgatcgcaa ttaacgccac gcttgaggta acagagattg ttttacctgc tggggagtgg
     7501 cacgccattc ccccattcgc tggagaggat aacccagtga ttacggctgt ctggcaggga
     7561 cctgcacacg gattgtgtgt gttccagaga tgataaaaaa ggagttagtc atggttagtt
     7621 tagagaagaa cgatcactta atgttggcgc gccagctgcc attgaaatct gttgccctga
     7681 tactggcggg aggacgtggt acccgcctga aggatttaac caataagcga gcaaaaccgg
     7741 ccgtacactt cggcggtaag ttccgcatta ttgactttgc gctgtctaac tgcatcaact
     7801 ctgggatccg tcgtatgggc gtgatcaccc agtaccagtc ccacactctg gtgcagcaca
     7861 ttcagcgcgg ctggtcattc ttcaatgaag aaatgaacga gtttgtcgat ctgctgccag
     7921 cacagcagag aatgaaaggg gaaaactggt atcgcggcac cgcagatgcg gtcacccaaa
     7981 acctcgacat tatccgccgt tataaagcgg aatacgtggt gatcctggcg ggcgaccata
     8041 tctacaagca agactactcg cgtatgctta tcgatcacgt cgaaaaaggc gcacgttgca
     8101 ccgttgcttg tatgccagta ccgattgaag aagcctccgc atttggcgtt atggcggttg
     8161 atgagaacga taaaattatc gaattcgttg aaaaacctgc taacccaccg tcaatgccga
     8221 acgatccgag caaatctctg gcgagtatgg gtatctacgt ctttgacgcc gactatctgt
     8281 atgaactgct ggaagaagac gatcgtgatg agaactccag ccacgacttt ggcaaagatt
     8341 tgattcccaa gatcaccgaa gccggtctgg cctatgcgca cccgttcccg ctctcttgcg
     8401 tacaatccga cccggatgcc gagccgtact ggcgcgatgt gggtacgctg gaagcttact
     8461 ggaaagcgaa cctcgatctg gcctctgtgg tgccggaact ggatatgtac gatcgcaatt
     8521 ggccaattcg cacctacaat gaatcattac cgccagcgaa attcgtgcag gatcgctccg
     8581 gtagccacgg gatgaccctt aactcactgg tatccggcgg ctgtgtgatc tccggttcgg
     8641 tggtggtgca gtccgttctg ttctcgcgcg ttcgcgtgaa ttcattctgc aacattgatt
     8701 ccgccgtatt gttaccggaa gtatgggtag gtcgctcgtg ccgtctgcgc cgctgcgtca
     8761 tcgatcgtgc ttgtgttatt ccggaaggca tggtgattgg tgaaaacgca gaggaagatg
     8821 cacgtcgttt ctatcgttca gaagaaggca tcgtgctggt aacgcgcgaa atgctacgga
     8881 agttagggca taaacaggag cgataatgca ggttttacat gtatgttcag agatgttccc
     8941 gctgcttaaa accggcggtc tggctgatgt tattggggca ttacccgcag cacaaatcgc
     9001 agacggcgtt gacgctcgcg tactgttgcc tgcatttccc gatattcgcc gtggcgtgac
     9061 cgatgcgcag gtagtatccc gtcgtgatac cttcgccgga catatcacgc tgttgttcgg
     9121 tcattacaac ggggttggca tatacctgat tgacgcgccg catctctatg atcgtccggg
     9181 aagcccgtat cacgatacca acttatttgc ctataccgac aacgtattgc gttttgcgct
     9241 tctggggtgg gttggggcag aaatggccag cgggcttgac ccattctggc gtcctgatgt
     9301 ggtgcatgcg cacgactggc atgcaggcct tgcgcctgcg tatctggcgg cgcgcgggcg
     9361 tcctgcgaag tcggtgttta ctgtgcacaa cctggcctat caaggcatgt tttatgcaca
     9421 tcacatgaat gacatccaat tgccatggtc attctttaat attcatgggc tggaattcaa
     9481 cggacaaatc tctttcctga aggccggtct gtactatgcc gatcacatta cggcggtcag
     9541 tccaacctac gctcgcgaga tcaccgaacc gcagtttgcc tacggtatgg aaggtctgtt
     9601 gcaacagcgt catcgtgaag ggcgtctttc cggcgtactg aacggcgtgg acgagaaaat
     9661 ctggagtcca gagacggact tactgttggc ctcgcgctac acccgcgata cgttggaaga
     9721 taaagcggaa aataagcgcc agttacaaat cgcaatgggg cttaaggttg acgataaagt
     9781 gccgcttttt gcagtggtga gccgtctgac cagccagaaa ggtctcgacc tggtgctgga
     9841 agccttaccg ggtcttctgg agcagggcgg gcagctggcg ctactcggcg cgggcgatcc
     9901 ggtgctgcag gaaggtttcc ttgcggcggc agcggaatac cccggtcagg tgggcgttca
     9961 gattggctat cacgaagcat tttcgcatcg cattatgggc ggcgcggacg tcattctggt
    10021 gcccagccgt tttgaaccgt gcggcttaac gcaactttat ggattgaagt acggtacgct
    10081 gccgttagtg cggcgcaccg gtgggcttgc tgatacggtt tctgactgtt ctcttgagaa
    10141 ccttgcagat ggcgtcgcca gtgggtttgt ctttgaagat aataatgcct ggtcgctgtt
    10201 acgggctatt cggcgtgctt ttgtactgtg gtcccgtcct tcactgtggc ggtttgtgca
    10261 acgtcaggct atggcaatgg attttagctg gcaggtcgcg gcgaagtcgt accgtgagct
    10321 ttactatcgc ttgaaatagt tttcaggaaa cgcctatatg aatgctccgt ttacatattc
    10381 atcgcccacg cttagcgtag aagctcttaa gcactctatc gcttacaagc tgatgtttac
    10441 gattggaaag gacccggtcg tcgccaataa acatgaatgg ctgaacgcaa cgttatttgc
    10501 tgtgcgcgat cgtctcgtgg agcgctggtt acgttcaaac cgtgcccagt tgtcgcaaga
    10561 aacacgtcag gtttactacc tgtcgatgga gtttttgatt ggccgtacgc tctccaacgc
    10621 catgttgtcg ctaggaattt acgaagatgt acagggcgca ctggaagcga tggggttaaa
    10681 tctcgaagag ctgattgatg aagaaaatga cccaggcctc ggtaacggtg gcctgggacg
    10741 tctggcggct tgcttccttg attctctggc gacgttaggg ttgccggggc gcggttacgg
    10801 catccgctat gactacggta tgttcaagca gaacatcgtt aacggtagcc agaaagagtc
    10861 gccagactac tggctggaat acggtaaccc gtgggaattc aaacgccaca acacgcgcta
    10921 taaagtccgt tttggcggtc gcattcagca ggaaggtaaa aaaacgcgct ggattgaaac
    10981 cgaagagatt ctgggagtcg cttacgatca gataatccct ggttacgaca ccgacgcgac
    11041 caacacgctg cgtttgtgga gtgcgcaagc cagtagcgaa attaacctcg gtaaattcaa
    11101 ccagggtgac tacttcgcgg cagtggaaga taaaaaccac tccgagaacg tatctcgcgt
    11161 actgtatccg gatgactcca cctactccgg gcgtgagctg cgcctgcgtc aggaatactt
    11221 cctggtttcc tcgaccattc aggacatttt aagccgccat tatcagttgc ataaaaccta
    11281 cgataacctg gcggataaaa tcgcgattca tctcaatgat acccatccgg tactgtcgat
    11341 tcctgagatg atgcgtctgc tgatcgatga gcaccaattt agctgggacg acgcgtttga
    11401 ggtgtgttgt caggtcttct cctacactaa ccacacgctg atgagcgagg cgctggaaac
    11461 ctggccggtt gatatgctgg gtaaaattct gccgcgtcac ctgcagatca tctttgaaat
    11521 caacgactat ttcctgaaaa ccttgcagga acagtatccg aacgataccg atctgctggg
    11581 acgggcgtcg atcattgatg aatccaacgg tcgtcgtgtg cgtatggcct ggctggcggt
    11641 tgttgtgagc cacaaagtta acggtgtatc ggaactgcac tctaatctga tggtgcaatc
    11701 gttgtttgcc gactttgcga aaatcttccc gggtcgtttc accaacgtca ccaacggtgt
    11761 gacgccgcgt cgctggctgg cggtagcgaa cccatcgctt tcagccgtgc tggacgaaca
    11821 cctgggccgt aactggcgca ccgaccttag cctgcttaat gagctgcaac aacactgtga
    11881 tttcccaatg gttaatcacg ctgtgcatca ggcgaagctg gagaacaaaa agcgtctggc
    11941 agagtatatc gcccagcagc tgaatgtggt ggtgaatcca aaggcgttgt tcgatgtaca
    12001 aatcaaacgt attcacgaat acaaacgtca attgatgaat gtgttgcatg tgattacccg
    12061 ctataaccgc atcaaggccg acccggatgc gaagtgggta ccgcgcgtga atatttttgg
    12121 cggtaaggcg gcttcggcct attacatggc gaagcacatt attcatttga tcaatgacgt
    12181 agcgaaagtg atcaacaacg atccgcagat tggcgataag ctgaaagtcg tgttcatccc
    12241 gaactacagc gttagcctgg cgcagttgat cattccggcg gcagatctgt ctgaacagat
    12301 ttcgctggca gggacggaag cttccggcac cagtaacatg aagtttgcgc ttaacggtgc
    12361 gctgactatc ggtacgttgg acggtgcgaa tgtcgagatg cgggatcatg tcggtgctga
    12421 caatatcttt atttttggta acacagcgga agaagtggaa gaactgcgtc gtcagggcta
    12481 caaaccgcgt gaatactacg agaaagatga ggagctgcat caggtgctga cgcaaatcgg
    12541 cagcggtgta ttcagtccgg aagatccggg tcgctatcgc gatctggttg attcgctgat
    12601 caacttcggc gatcactatc aggttctggc ggattatcgc agctatgtcg attgtcagga
    12661 taaagtcgac gaactctacg agcttcagga agagtggacc gcaaaagcga tgctgaacat
    12721 tgccaatatg ggctacttct cttctgaccg tactatcaaa gagtacgccg atcatatctg
    12781 gcatatcgat ccggtgagat tgtaagttca ccaataaata gaacggggcc aaagg
//
LOCUS       DAJVRF010000117        12826 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJVRF010000117 DAJVRF010000000
VERSION     DAJVRF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12826
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.117"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(133..1782)
                     /gene="actP"
                     /locus_tag="OQN13_003033"
     CDS             complement(133..1782)
                     /gene="actP"
                     /locus_tag="OQN13_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCS1616324.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1779..2092)
                     /locus_tag="OQN13_003034"
                     /pseudo
     CDS             complement(1779..2092)
                     /locus_tag="OQN13_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2292..4250)
                     /gene="acs"
                     /locus_tag="OQN13_003035"
     CDS             complement(2292..4250)
                     /gene="acs"
                     /locus_tag="OQN13_003035"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCS1616325.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4644..6080
                     /gene="nrfA"
                     /locus_tag="OQN13_003036"
     CDS             4644..6080
                     /gene="nrfA"
                     /locus_tag="OQN13_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCS1616326.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6125..6691
                     /gene="nrfB"
                     /locus_tag="OQN13_003037"
     CDS             6125..6691
                     /gene="nrfB"
                     /locus_tag="OQN13_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCS1616327.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6688..7359
                     /gene="nrfC"
                     /locus_tag="OQN13_003038"
     CDS             6688..7359
                     /gene="nrfC"
                     /locus_tag="OQN13_003038"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCS1616328.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7356..8312
                     /gene="nrfD"
                     /locus_tag="OQN13_003039"
     CDS             7356..8312
                     /gene="nrfD"
                     /locus_tag="OQN13_003039"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCS1616329.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8332..10050
                     /locus_tag="OQN13_003040"
     CDS             8332..10050
                     /locus_tag="OQN13_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCS1616330.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSHSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10043..10426
                     /gene="nrfF"
                     /locus_tag="OQN13_003041"
     CDS             10043..10426
                     /gene="nrfF"
                     /locus_tag="OQN13_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCS1616331.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10423..11019
                     /gene="nrfG"
                     /locus_tag="OQN13_003042"
     CDS             10423..11019
                     /gene="nrfG"
                     /locus_tag="OQN13_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCS1616332.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11361..12674
                     /gene="gltP"
                     /locus_tag="OQN13_003043"
     CDS             11361..12674
                     /gene="gltP"
                     /locus_tag="OQN13_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCS1616333.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
BASE COUNT         2854 a         3346 c         3574 g         3052 t
ORIGIN      
        1 attatcattt tctacgactg tcaaaaatcg ctcatttttt aatgagttta tttgtttaat
       61 attttgggaa aaggtgatgc atgctggaga ggaagagtat tccccggtca ggcgaccggg
      121 gaaggggaga gattaatgtg cgcggccttg ctcaacgcca aagccggtct gggagcggat
      181 aaactgcgcg cggaacagtt cacgctcgcg cgccccttcc gctgagttat cggttgccga
      241 gaagaaccag atgccgagga atgccacggt gatagagaac agcgccgggt attcatacgg
      301 gaagatggct ttttcgtgac caaggatctg tacccaaatc gtcgggccga ggatcatcag
      361 tactactgcg gtaatcagcc ccagccagcc acccatcatc gcgccacgcg tggtcagttt
      421 cgaccagtac atagaaagca gaatgatcgg gaagttacag ctcgccgcga tggcaaacgc
      481 cagccccacc ataaaggcga tgttctgatt ctcaaacagc acgccgagga taatcgcaat
      541 cacgccgagg atcagtacgg tgatttttga tacccgcagc tcttcacgtt cggtcgcgcc
      601 ttttttgaag acgttagcgt acaagtcatg cgaaaccgcg gatgcgcccg ccagcgtcag
      661 acccgcaacc accgcgagga tagtggcgaa agcaaccgct gaaataaaac cgaggaacag
      721 gttgccgccc actgcattcg ccaggtgaac ggccgccatg ttgttaccac caatcagatg
      781 gcccgccgcg tctttatatt ccggattcgc accaaccagc atgatcgcgc cgaagccgat
      841 aataaaggtc agaatataga agtagcccat aaacccggtg gcgtagaaca cgctcttacg
      901 tgcttcgcgg gcatcgctga ctgtaaagaa gcgcatcaga atgtgcggca agcccgccgt
      961 accaaacatc agtcccagac ccagagagag tgcggagatc ggatctttca ccagcccgcc
     1021 cggcttcatg atgtcgacac ctttcgggtg taccgccatc gcttcactga acagattgtt
     1081 gaagctaaag ccgacgtgtt tcatcaccat aaaggccata aagctggcac cgaacagcaa
     1141 cagcacggct ttgataatct gtacccaagt ggtcgccagc atgccgccga acaggacgta
     1201 catcatcatc agcacgccga ccagcaccac cgcaatgtga tagttaaggc caaacagcag
     1261 ctcgatcagt ttacctgcgc ccaccatctg ggcgataagg taaagcgcca ccaccaccag
     1321 agaaccacag gccgaaagaa tacgaatcgg cccttgtttc agacggtaag aggccacatc
     1381 ggcaaaggtg tagcgcccca ggttacgcag acgttcggca atcaggaaca aaatgatcgg
     1441 ccagcccacc aggaagccca gcgagtaaat taagccgtca tagccggagg taaacaccag
     1501 cgcggagatc cccaagaatg aggcggcgga catatagtcc ccggcaatcg ccagcccgtt
     1561 ctggaagcca gtgatattgc cgcctgcggt gtagtagtcg ctacgagaac gtacgagttt
     1621 tgatgcccag taggtaatgc cgagcgtaaa cacgacgaaa atcaggaaca taataatcgc
     1681 ctgccagttc gttggctggc gctctacggc cccgctaata gcatccgcgg cgttagctgc
     1741 gaaagggagt gtggcggcaa gcgccgtcag aactcttttc atgatgcttg tacctcatgc
     1801 aggacttcat tattaagacg gtcgaattcg ccgttcgccc gccagatgta gataccggtg
     1861 agaacaaagg agatcacaat cactccaaca ccaattggaa taccgcgtgt gacgctggtg
     1921 ttcggattca gcggcgtgcc agccagccgg gcgcgaaggc gatcagtaaa ataaagccga
     1981 tataaactgc cagcataata atcgacagga tggtggcaaa ccgttgccgt ttttcgacta
     2041 actccctgaa atgcgcattg tcttctatcc gctgataaat agtgccattc atcacagatt
     2101 ctccagaggt aatgtaggga ttgttttaat tcccgctccc ttatgggaga aggttaacgc
     2161 tcgggtaacc cttgccgaat gtaggccgga taaggcgttt acgccgcatc cggcaatcaa
     2221 tgcctgatgc gacgctgtcg cgtcttatca ggcctacaaa ccgttaccga ctcgcatccg
     2281 gcaattgtgg gttacgatgg catcgcgata gcctgcttct cttcaagcag cttctcgact
     2341 acgccaggat cggcaagcgt cgaggtatcg cccaggttgc tggtatcgcc cgccgcaatt
     2401 ttgcgcagaa tacggcgcat aattttgccg gagcgggttt taggcaggga gtcggtccag
     2461 tgcagcacgt ctggcgtcgc cagcgggcca atctctttac gcacccagtt gcggacttct
     2521 gcgtacagtt ctggtgacgg ttcctccccg tgattaagcg tgacgtaggc gtagatcgcc
     2581 tgacctttaa tattgtgcgg aatacctact acggcggctt cggcaatctt cggatgcgcc
     2641 accagcgccg actcaatctc tgccgtcccc agacggtgac cggagacgtt cagcacgtcg
     2701 tccacacgcc cggtgatcca gtaatagcca tcttcatcgc gacgcgcgcc gtcgccgctg
     2761 aaatacatat ttttgaaggt ggagaagtag gtctgttcaa aacgttcgtg atcgccaaac
     2821 agcgtacgcg cctgacccgg ccaggagtcg gtgatcacca ggctgccttc ggtagcgcct
     2881 tccagcgggt taccttcgtt atcgaccagc gccggttgca cgccgaagaa cggacgtgtt
     2941 gccgaaccgg ctttcagctc ggtagcgcca ggcagcggcg tgatcatgaa accgccagtt
     3001 tcggtctgcc accaggtatc gaccaccgga catttctcgt tgccgatttt tttccagtac
     3061 cactcccacg cttccgggtt aattggctcg cccacggaac cgagaatgcg cagcgacgaa
     3121 cggtcggtac cctcgatcgc tttatcgcct tccgccatca gcgcgcggat cgccgtgggt
     3181 gcggtataga gaatattgac ctgatgcttg tccaccacct gcgccatacg ggcaggcgtc
     3241 ggccagttgg gtacgccttc aaacatcagc gtggtcgcac cgcaggccag cgggccgtac
     3301 agcaagtaac tgtgtccggt cacccagccc acatcggcgg tgcaccagta gatatcaccc
     3361 ggatgataat caaagacata tttaaaggtc agcgccgcgt acaccagata accgccggta
     3421 gtatgcagca caccttttgg cttaccggta gaaccggagg tgtagagaat aaacagcgga
     3481 tcttcggcgt tcatcttttc cgcctggtgc tgatcgctcg cttgctcaac caggtcgtgc
     3541 caccacaggt cgcgcccttc ctgccagtca attttcccgc cagtacgctt cagtaccacc
     3601 acatgctcta cgctggtgac gttcgggttt ttcagcgcgt catcaacgtt tttcttcagc
     3661 ggaatactgc gcccggcacg cacaccttcg tcggaagtga tcaccagtcg tgagttggaa
     3721 tcaataatgc gcccggcaac ggcttccggc gagaagccgc cgaaaatcac cgaatgcacc
     3781 gcgccaatgc gggcgcaggc cagcatcgca accgcggctt ccggcaccat cggcatataa
     3841 atcgccacca catcaccttt tttaatgccc agctcgagca gggtattggc gaagcggcag
     3901 acgtcgcggt gcagctcttt atagctgata tgtttgctct ggctggcgtc gtcgccttcc
     3961 cagatgatgg cggtacgatc gccgttttct tgcagatggc ggtcaaggca gtttgccgcc
     4021 agattcagcg tgccgtcctc gtaccattta atggacacat taccgggggc aaaggaggtg
     4081 tttttcacct tctggtaagg tttgatccag tcaagaattt ttccctgttc gccccagaag
     4141 gtatcaggta cgttaataga ttgttgatac atcgcctcgt actgctgagg gtttatcagg
     4201 caacggtctg cgatgttggc aggaatggtg tgtttgtgaa tttggctcat gcttttgttc
     4261 tccttgtagg gtgttaataa tatgtggcat aagcgttaaa tgtaggggta ttggcagttt
     4321 tgtttagtat ttgggcgaca gatcacgcaa aagtagaatt gtgcaaataa acggcagggt
     4381 aatttttgaa ggtcagcaac aaaagttgat taattctttg aggaacatgc agttatgcat
     4441 tgctgttgaa aaagaggaag atactaacta actctaaagt ggtattttac atgcacttac
     4501 aattgattaa agacaacatt cacagtgtgg ttatttgtta cacatagggg cgagcaatgt
     4561 catgacagtg taggtgcggt tactgtcgtg aaaaacaata aaaaccgcca ttgtaacaat
     4621 ggcgcaattc ggatgaagcc cctatgacaa ggataaaaat aaacgcacgc cgtatcttca
     4681 gcttattgat tccttttttc tttttcactt ctgttcacgc tgaacaaacg gcagctcccg
     4741 caaaacctgt aactgtggaa gcgaagaatg aaacctttgc cccgcagcat cccgatcaat
     4801 atctctcctg gaaagccacc tcggaacagt cagagcgtgt tgacgccctg gcggaagatc
     4861 cacggctggt gatcctgtgg gcggggtatc ccttctcgcg cgattacaac aagccgcgtg
     4921 gacatgcctt tgctgtgacc gatgtgcgtg aaaccctgcg taccggtgcg ccgaaaaacg
     4981 ctgaagatgg tccgctaccg atggcgtgct ggagttgtaa aagcccggat gtggcgcgtc
     5041 tgatccagaa agacggcgaa gatggctact tccacggtaa gtgggcgcgc ggcggcccgg
     5101 aaatcgtcaa caacttaggt tgtgccgact gccataacac cgcctcacca gagttcgcca
     5161 aaggcaaacc ggagttaacc ctttcccgtc cgtatgcggc tcgcgcgatg gaagccattg
     5221 gtaaaccttt tgagaaagcc ggacgtttcg accagcaatc gatggtttgc ggtcagtgcc
     5281 atgtggagta ttacttcgac ggcaaaaaca aagcggttaa attcccgtgg gatgacggca
     5341 tgaaagtcga aaatatggag cagtattacg acaaaattgc cttctctgac tggactaact
     5401 ccctgtcgaa aacgccaatg ctgaaagcgc agcacccgga atatgaaacc tggacagcgg
     5461 gcattcacgg taaaaacaac gtgacctgta tcgactgcca tatgccaaaa gtgcagaacg
     5521 ccgaaggcaa actctatacc gaccataaaa ttggtaatcc gtttgataac ttcgcccaga
     5581 cttgtgcgaa ctgccatacc caggacaaag ctgccttgca aaaagtggtc gcggaacgta
     5641 agcagtcgat taacgacctg aaaatcaagg ttgaagatca actggttcac gctcacttcg
     5701 aagcgaaagc agcgctggat gcaggcgcga cggaagctga aatgaagcca attcaggacg
     5761 atatccgtca tgcccagtgg cgctgggatc tggcgatcgc ttcccacggc attcatatgc
     5821 acgcaccgga agaaggttta cggatgctcg gtacggcgat ggataaagcg gcggatgcac
     5881 gcaccaaact ggcgcgcctg ctggcgacca aaggcatcac ccatgaaatc cagatcccgg
     5941 atatctcaac caaagagaaa gcccagcagg ccattggcct gaacatggaa caaatcaagg
     6001 ccgagaagca ggacttcatc aaaacggtga tcccgcagtg ggaagaacag gcacgtaaaa
     6061 acggtctgtt aagccaataa ccccgttccg cctcgcaagg ggcggaaaac acaatggagt
     6121 gaatatgagc gtattacgtt cgttattaac tgccggggtg ctggcgtcgg gcctgttgtg
     6181 gagcctgaac ggaattaccg ctacccctgc ggcgcaggca tctgatgatc gctacgaagt
     6241 tacccagcag cgtaacccgg atgccgcctg tctggactgt cataaaccag ataccgaagg
     6301 tatgcatggc aaacatgctt ccgttatcaa cccgaataac aaactgccgg tcacctgcac
     6361 caactgtcac ggccagccat caccgcaaca ccgcgaaggg gtgaaagatg tgatgcgctt
     6421 taacgagccg atgtacaagg ttggagagca gaacagcgtc tgtatgtcct gtcatctgcc
     6481 ggaacagttg caaaaagcgt tctggccgca cgatgtccac gtaaccaaag tggcgtgcgc
     6541 cagctgccat tccctgcatc cacagcaaga tacgatgcag acgttaagtg acaaaggacg
     6601 gattaagatt tgcgtcgatt gccacagcga tcagcgcacc aatccgaact ttaacccggc
     6661 gtctgttccg ttgcttaagg agcagccatg acctggtctc gtcgccagtt tctcaccggc
     6721 gtcggcgtgc tggcagccgt cagcggcacc gcagggcgcg tggtggcgaa aacgttgaat
     6781 atcaatgggg tgcgttacgg catggtgcat gacgagtcgt tatgcatcgg ctgtaccgcc
     6841 tgtatggatg cttgtcggga agtgaacaag gtgccggaag gcgtctcgcg cctgacgatt
     6901 attcgtagcg agccgcaggg cgaatttcct gatgtgaaat atcgcttctt ccgtaagtct
     6961 tgccagcact gcgatcatgc gccgtgcgtt gacgtctgcc cgaccggtgc gtcttttcgt
     7021 gatgccgcta gcgggattgt cgatgttaat cctgatctct gcgtcggttg tcagtactgc
     7081 atcgccgcct gtccgtaccg cgtgcgcttt atccatccgg tcacgaagac ggcggacaaa
     7141 tgcgatttct gccgtaaaac caatttgcag gccggtaagc tgcccgcgtg tgttgaggct
     7201 tgcccgacga aggcgctgac gtttggcaat ctggacgatc ctaacagtga gatttcgcaa
     7261 ctgctgcgcc agaagcctac ttaccgctac aagctggcgc tgggaaccaa accgaagctg
     7321 taccgcgtac cgtttaaata cggggaggtg agccaatgac gcagacttcc gcatttcatt
     7381 ttgaatcgct ggtgtgggac tggccgattg ccatctacct gtttttgatt ggtatttctg
     7441 ccggtctggt gacgctggcc gtgctgttac gtcgcttcta cccgcaggcg ggcggtgcag
     7501 acagtacgtt gctgcgcacc acgctgattg tcgggccggg cgcggtgatc ctcggtctgt
     7561 tgatcctcgt cttccacctg acaagaccgt ggaccttctg gaagctgatg ttccactaca
     7621 gttttacctc ggtgatgtcg atgggggtga tgctgtttca gctctacatg gtggtgctgg
     7681 tgctgtggct ggcgaaaatc tttgaacatg atttgcttgc cctgcaacaa cgctggttgc
     7741 cgaagctggg gatcgtgcaa aaggttctga gcctgctgac gcccgttcat cgcggactgg
     7801 aaacgctgat gctggtgttg gcggtgctgc tgggggctta taccggcttt ctgctgtcgg
     7861 cgctgaaatc gtatccgttc ctcaataacc cgatcctgcc ggtgctgttc ctcttctccg
     7921 gcatctcgtc cggtgcggcg gtggcgctga tcgccatggc gatacgccaa cgcagtaacc
     7981 cgcattccac ggaagcgcac tttgttcacc ggatggaaat ccccgtggta tggggtgaaa
     8041 tcttcctgct ggtggcgttt tttgtcggtc tggcgctggg cgatgacggt aaagttcgtg
     8101 cgctggtggc ggcattaggt ggcggtttct ggacgtggtg gttctggctt ggtgtcgccg
     8161 ggctggggct gattgtgcca atgttgctca aaccgtgggt caatcgcagt tccggcattc
     8221 ctgccgtgct ggcggcgtgt ggggccagtc tggtcggcgt gttgatgctg cgctttttca
     8281 ttctctacgc cggacagttg acggtggcgt aagccagaaa agaggtggtt tctggacgta
     8341 ttccttcctg aagtcggttt tctggcgttg ttgttaagtc tcggggtcaa cgtgttgacc
     8401 ccgttgacgg cctttgcggg agtgcggttg cgctggcctg ccatgatgcg actcacttgc
     8461 atcggcattc tggcgcagtt cgcgctcctg ctgctcgcct ttggcgtact gacgtattgt
     8521 tttctcatca gcgatttctc ggtcatttat gtcgcgcaac atagccatag cctgctgtcg
     8581 tgggaattca agctggcagc ggtgtggggc ggccatgaag gttcgctgct gctttgggtg
     8641 ctgctgcttt ccgcctggag cgcgctgttt gcctggcatt atcggcagca aaccgatccg
     8701 ctatttccgc tgacgttagc cgttttatct ctcatgctcg ccgcactgct actgtttgtg
     8761 gtgctgtggt ccgatccctt cgtgcggata tttccaccag caatcgaagg ccgcgatctc
     8821 aatccgatgc tgcaacatcc cggtcttatc tttcatccac cgctgcttta ccttggctat
     8881 ggcggtttga tggtagcggc gagcgtggcg ctggcgagtc tactgcgcgg cgagtttgat
     8941 gcggccagcg cccgaatttg ctggcgctgg gcgttacctg gctggagtgc attaacggcg
     9001 gggatcatcc tcggttcctg gtgggcctac tgcgaactcg gctggggcgg ctggtggttc
     9061 tgggatccgg tggaaaatgc ctctttatta ccctggcttt ctgccactgc gctgctgcac
     9121 agtttatccc tgacacgcca gcgggggatt ttttgccact ggtcgctgtt gctggcgata
     9181 gttactctga tgctgtcgct gctgggcacc ttaattgtcc gttctggcat tctggtttcg
     9241 gttcatgcgt tcgcactgga taacgtccgc gccgtgccgt tgttcagcct gtttgcactg
     9301 attagccttg cgtctttggc tctgtatggc tggcgagcgc gggacggtgg cccggcggtg
     9361 cgtttttcgg ggttatcgcg ggaaatgtta atcctcgcta cgctgttgct gttttgcgca
     9421 gtgctactga tcgtgctggt gggaacgctt tatccgatga tttacggtct gctgggctgg
     9481 ggacgcctct ccgttggcgc gccgtatttt aaccgcgcga cgttaccgtt tggtctgttg
     9541 atgctggtgg tgattgtgct ggcgacgttt gtctctggca aacgcgcgca gcttccggcg
     9601 ctggtcgctc atgcgggcgt gctgttattt gccgcaggga tcgtggtttc cagcgtcagc
     9661 cgtcaggaga tcagcctgaa tttacagccg ggtcagcagg tgacgctggc aggatacacc
     9721 ttccgttttg agcgcctcga tctgcaagcc aaaggcaatt acaccagcga aaaagcgata
     9781 gtggcgctgt ttgaccatca gcaacgcatt ggtgaattaa cgccggagcg gcgtttttat
     9841 gaagcacgcc gtcagcaaat gatggaaccg tcaattcgct ggaacggcat ccatgactgg
     9901 tatgcggtca tgggtgaaaa aaccggagcg gatcgttacg cttttcgttt gtatgtacaa
     9961 agcggtgtgc gctggatctg gggtggagga ttgttgatga ttgcgggcgc attgttaagc
    10021 ggatggcggg ggaagaagcg cgatgaataa agggcttctc acgctactgc tgttatttac
    10081 ctgttttgcg cgcgctcagg tcgtagacac ctggcaattc gccaatccgc aacaacagca
    10141 acaggcgtta aatattgcca gccagttacg ttgtccgcag tgccagaatc aaaacttact
    10201 cgaatccaat gcgccggtgg cggtcagtat gcgccatcag gtttatagca tggtgtcgga
    10261 ggggaagaac gaagtcgaaa tcattggctg gatgaccgaa cgctacggag attttgttcg
    10321 ctataaccca ccgttaacgg gtcagacgct ggtgttgtgg gcgctgccag tggtgttgtt
    10381 attgctgatg gcactgatcc tctggcgagt gagggctaag cgatgaaaca gcctcaaata
    10441 ccggtgaaaa tgctgacaac actcacgata ttgatggtat tcctctgtgt cggcagttat
    10501 ctgttaagcc caaaatggca ggcggtacgt gcggagtatc agcgtcagcg cgatccgcta
    10561 catcagtttg ccagccagca aaccccggaa gcgcagcttc aggcattgca ggataaaatc
    10621 cgcgctaatc cacaaaacag cgaacaatgg gcgttactgg gcgagtacta tctgtggcaa
    10681 aacgattaca gcaattcgct gctggcgtat cgtcaggcgt tgcaactacg tggtgagaac
    10741 gctgaactgt atgcggcgct ggcgacggtg ctttattacc aggccagcca gcatatgacc
    10801 gcccagactt gcgcaatgat cgacaaagcc ctcgcgctgg actctaacga aatcaccgct
    10861 ctgatgctgc tggcttccga tgcgtttatg caggcgaatt acgcgcaagc catcgaacta
    10921 tggcaaaaag tgatggatct caactcaccc cggatcaacc ggacacagct ggtcgagtcg
    10981 attaatatgg caaaactgtt gcagcggaga tcggattaat catctggtga tgaagattat
    11041 gtcattgagc tgcataaaaa ataatcgaat gaacatatgc caaaaataat cactaatcag
    11101 tattattgca gattaaacaa ataaaaatct ttccataaca aatggttatt cattaatcct
    11161 gccataaact cgcgttatta tgcattaatg cagcgaaaag ctctgttgtt aaagggttgc
    11221 gcaacatacc gcgcaaatga tactgatcat aagcgttaaa aaaatctaca aaccaacgca
    11281 acacaattca tgccctggca gtatgtcacg ttctcgcgtt tctgaacggg gaacggcgct
    11341 ccattgagga agttattctg atgaaaaata taaaattcag cctggcctgg cagattctgt
    11401 ttgctatggt gctgggcatt ctcctgggaa gctacctgca ctaccatagc gacagccgcg
    11461 actggctggt cgtcaatttg ctctctccgg cgggtgatat cttcatccat ctgatcaaaa
    11521 tgattgttgt gccgattgtg atctccacgc tggtggtggg tatcgcgggt gttggtgatg
    11581 ccaaacagct cgggcgtatt ggcgcgaaaa ccattattta cttcgaggtg atcaccaccg
    11641 tcgccatcat tttggggatc actctggcga acgtcttcca gcccggtgcc ggggtggata
    11701 tgtcgcagct ggcgaccgtc gatatctcga aatatcagag cactacggaa gcggtacaaa
    11761 gcagttccca cggcattatg ggcacgattt tgtcgctggt gccgacgaac attgtggcgt
    11821 cgatggcgaa aggcgaaatg ctgccgatca tctttttctc ggtgctgttt ggtctggggc
    11881 tttcttccct gcccgcgacg catcgtgaac cgctggtgac cgtgttccgc tccatctctg
    11941 aaaccatgtt taaagtgact cacatggtga tgcgttatgc accggtgggt gtgtttgcgc
    12001 tgattgcggt gacggtggct aactttggtt tctcttctct gtggccactg gcgaaactgg
    12061 tgctgctggt gcatttcgcc attctgttct tcgcgctggt agtgctggga attgtggcgc
    12121 gcctgtgcgg gttaagcgtc tggatcctga ttcgtattct gaaagatgag ctgattctgg
    12181 cgtactccac tgccagctct gaaagcgtac tgccgcgaat tattgagaag atggaagcct
    12241 acggagcacc ggcgtcgatc accagcttcg tggtgccgac cggttactct tttaaccttg
    12301 atggttcgac gctgtatcaa agtattgccg ctattttcat cgctcagctg tatggcattg
    12361 acctgtccat ctggcaggaa atcattctgg tgctgacgct gatggtgacc tcgaaaggga
    12421 ttgctggcgt gccgggcgtg tcgtttgtgg tgctgctggc aacgctgggt agcgtaggta
    12481 ttccgctgga aggtctggcg tttattgctg gtgttgaccg tatcctcgac atggcgcgta
    12541 ctgcgctgaa cgtggtgggt aatgcgctgg cggtgctggt gattgccaag tgggaacaca
    12601 aatttgaccg caaaaaggcg ctggcttatg agcgtgaagt gctgggcaaa tttgataaaa
    12661 ctgcggatca ataattgaag attgccgggg atatccaccc ggcaatgtgt gaatgcctga
    12721 tgcgacgctt gccgcgtctt atcaggccta ccttaaccgt tggttaattt ttcaaactct
    12781 tcacacccgg tatcaaaccc ttccatacag ctcaggttca accagt
//
LOCUS       DAJVRF010000118        12349 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJVRF010000118 DAJVRF010000000
VERSION     DAJVRF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12349
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.118"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(111..320)
                     /locus_tag="OQN13_003044"
                     /pseudo
     CDS             complement(111..320)
                     /locus_tag="OQN13_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(522..1166)
                     /gene="cheZ"
                     /locus_tag="OQN13_003045"
     CDS             complement(522..1166)
                     /gene="cheZ"
                     /locus_tag="OQN13_003045"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCS1616334.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1177..1566)
                     /gene="cheY"
                     /locus_tag="OQN13_003046"
     CDS             complement(1177..1566)
                     /gene="cheY"
                     /locus_tag="OQN13_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCS1616335.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1581..2630)
                     /gene="cheB"
                     /locus_tag="OQN13_003047"
     CDS             complement(1581..2630)
                     /gene="cheB"
                     /locus_tag="OQN13_003047"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCS1616336.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2633..3493)
                     /gene="cheR"
                     /locus_tag="OQN13_003048"
     CDS             complement(2633..3493)
                     /gene="cheR"
                     /locus_tag="OQN13_003048"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCS1616337.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLMDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHAHRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGYSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3512..5113)
                     /gene="tap"
                     /locus_tag="OQN13_003049"
     CDS             complement(3512..5113)
                     /gene="tap"
                     /locus_tag="OQN13_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCS1616338.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5159..6820)
                     /gene="tar"
                     /locus_tag="OQN13_003050"
     CDS             complement(5159..6820)
                     /gene="tar"
                     /locus_tag="OQN13_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCS1616339.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6965..7468)
                     /gene="cheW"
                     /locus_tag="OQN13_003051"
     CDS             complement(6965..7468)
                     /gene="cheW"
                     /locus_tag="OQN13_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCS1616340.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7489..9453)
                     /gene="cheA"
                     /locus_tag="OQN13_003052"
     CDS             complement(7489..9453)
                     /gene="cheA"
                     /locus_tag="OQN13_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCS1616341.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9458..10384)
                     /gene="motB"
                     /locus_tag="OQN13_003053"
     CDS             complement(9458..10384)
                     /gene="motB"
                     /locus_tag="OQN13_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCS1616342.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRVIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10381..11268)
                     /gene="motA"
                     /locus_tag="OQN13_003054"
     CDS             complement(10381..11268)
                     /gene="motA"
                     /locus_tag="OQN13_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCS1616343.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11394..11972)
                     /gene="flhC"
                     /locus_tag="OQN13_003055"
     CDS             complement(11394..11972)
                     /gene="flhC"
                     /locus_tag="OQN13_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCS1616344.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11975..12325)
                     /gene="flhD"
                     /locus_tag="OQN13_003056"
     CDS             complement(11975..12325)
                     /gene="flhD"
                     /locus_tag="OQN13_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCS1616345.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         2829 a         3449 c         3016 g         3055 t
ORIGIN      
        1 attacatagg ggctcgcagc gttacggctg caataaagcc ggatataaag ctgcaaccta
       61 cccgtcatgt caaaacaatg gatgccttgc aaacgggatg cgttcatata aatactgtga
      121 tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga cacaccgcca
      181 aaccagataa cacacacgcc cactaacaaa atcagcaacg aggtcagttc acgggaacgc
      241 gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc ttctgttttg
      301 tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagctt aaatgatgcc
      361 agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt tagggctttt
      421 gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc cttatcagac
      481 cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc aagactatcc
      541 aacaaatcgt ccacctgatc ctgactggct accacgccga ctttgctggt atcgacctga
      601 ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc cgggatgttt
      661 tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat catccgctta
      721 atgaccagcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag cagttgcgcg
      781 ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc tgttaccagt
      841 tcacgggcat cggcaaggtc aatcggatcg gcaaaccagt catcccaacg ttgggttaac
      901 gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac actgttcagc
      961 gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc gcgcgcatcg
     1021 gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg caaactgtcg
     1081 cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga atgctcgtca
     1141 gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt tctcaaagat
     1201 tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat agccactggc
     1261 ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca ccattaacac
     1321 tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca ggccatccat
     1381 attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca acttattgag
     1441 agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt tcagcaggtt
     1501 acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa gttctttatc
     1561 cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat ttttgccaac
     1621 atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat attgatggcc
     1681 tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt ccatgccccc
     1741 gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt caggatcacc
     1801 ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc taccgaaggc
     1861 cgatgacggt taaccgccgg gccgtcgtga attttgattt gataatttgc gccactacgc
     1921 gccagttcca tatgccgatc gcccggcgca atataggcat gccccggcaa gacacgttct
     1981 ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc ggcaaaagag
     2041 cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga aagcggcaac
     2101 ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat cgcaatcagt
     2161 ttttcagaac tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa tggcttatgt
     2221 gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc gctatacgcc
     2281 agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc ccccagctcc
     2341 agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat cacaacgggc
     2401 attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg cggcatttca
     2461 acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc gaccagcgga
     2521 tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc tgtcatgatc
     2581 tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat cgttaatcct
     2641 tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg tggctaaagt
     2701 tttcagagta acccgcaaac agcaatccgt cgggtttaag gagcggaaca aagcggcgca
     2761 aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag aagatcgcat
     2821 caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca aaatcaacat
     2881 agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc cctcgcatga
     2941 aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga taaataccgc
     3001 ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc cagcgtccgg
     3061 gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct tcgccggtcg
     3121 aagccgccgc gctccatacg cgatactcgc cagaacggcg atgtgcgtga tccgcgagca
     3181 gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc gaattgataa
     3241 acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa tgaccgaaat
     3301 ccatcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg tcgcgtttat
     3361 ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc cgaaaatgcg
     3421 cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc ccacagggca
     3481 gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca atttgtaact
     3541 gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc acacgcgacg
     3601 aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct tctaccagcg
     3661 aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc tgctcaatcc
     3721 cccgctgttg ttcttccgac gcagaggcaa tttctcccat aatgtcgttc acgcgggtca
     3781 ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc gaaccctgct
     3841 ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc tgcgcgctac
     3901 ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc tgctctccgg
     3961 cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa gcaattccgt
     4021 cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc tgcatggtgt
     4081 gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca ttttttgcca
     4141 gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt aattgctcca
     4201 tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt gagagatcgt
     4261 tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc ttacgcacat
     4321 cacttaccgt cccacgcaaa gcctgctgca tggtcttcag actggcaaaa atggcggtga
     4381 tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca atgctgtcaa
     4441 aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac cacagcgcac
     4501 tgctgatgta gatcgccgca acaataatca tgctgataaa caccagtgtc gaaatctgat
     4561 agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc agctgccagg
     4621 cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg aggaaatccg
     4681 aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc cagcgggcaa
     4741 aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca gtcatcgcca
     4801 taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc gtcatcaacg
     4861 ttttaatgtc atccgccgga tagctaagtg ccgtcagagt gcccgctttg ttcagcgcgg
     4921 tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc tgattgctct
     4981 gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac atgccgttac
     5041 tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc gaaattcgaa
     5101 tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta aatactcgtc
     5161 aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt gccggtggtt
     5221 gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg gctgccagac
     5281 ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca gcggcggcgg
     5341 cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt tccgaaaccg
     5401 ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca atctcgccca
     5461 taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc ccggcgcttt
     5521 cgaccagcac cgaaccggta tcaacgcgtg cgacggagtc ttcaatgagg gctttgatct
     5581 cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcacccgcc accacggcaa
     5641 aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc gcgaggatat
     5701 tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc ttcgaactat
     5761 cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg tgctgggcgg
     5821 tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg ttttgcttca
     5881 ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat gcctgctgtt
     5941 cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg gcatagatgg
     6001 catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc atatgtgaaa
     6061 cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta ttcgctaggt
     6121 taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggagta agcaacatac
     6181 ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg ataaccgcca
     6241 gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg cgatacagtt
     6301 tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc attccctggg
     6361 ttggctgagc gaaataagct ccagtattgc cataatctag ataatcaatc agttcagtta
     6421 acgctgtgta atagttttta tatttttcat caatattacg actggtagcg accatttcag
     6481 gtaacggtgc catgcttttg aattttttat aatgcgtcgc tgcctgcgcc aatgttttcc
     6541 tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa tccatcatca
     6601 tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc caggttgacg
     6661 tcagctcgcc ctgctgttcc cgcaattgat tggaaaccac aaagctcttc tggctatggt
     6721 gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagtgcgaat acccccagca
     6781 ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt cctgaaaaca
     6841 agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca cccgccgcga
     6901 aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga ggaaatcggg
     6961 agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg ctgttcagca
     7021 gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcgcccagt ccagtgagat
     7081 attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc gccgtcaatg
     7141 aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg agattcagga
     7201 cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta attcgtaagt
     7261 caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac gctggcgtgt
     7321 tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg atatcaatac
     7381 cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc tcgctggcca
     7441 gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc aggcggcggt
     7501 gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa caatcagtgc
     7561 cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc gatagttact
     7621 ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca gcaaggcgta
     7681 gcggcgaccg ccactttgta agatcacaac aattccctgg gtggcttcgg ttttcgcgcc
     7741 cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc gcacttccag
     7801 tacccgctcg ccgccggcca gtggatggag atcggcttca cggggttgca gtgattccat
     7861 aacagcattc agcggcagaa tgaaaacttc atccccaacg cgtacggaca tgccgtcgag
     7921 gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtcccct gcttcgactg
     7981 gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca tgccgacgcc
     8041 gcgcccggag acgtcggtga cctgctctgc catggagaag cctggtgcaa atatcagcat
     8101 cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg ccgcttttgc
     8161 cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc aaatgttgcc
     8221 gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg cggcgagccg
     8281 tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg tcagcgggtc
     8341 gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa ctgcccacca gcgtcagttc
     8401 tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc gactaaaaac
     8461 atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac gggcgttacg
     8521 ttgtaactgc cccatgctgg ttatcaaatc accatgatta accgggtcca gttcgctgga
     8581 acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa ttaattgatc
     8641 aaccttttct accgctacac ggatgctggt ggattcattg ctgcgcgtcg ttttttcccg
     8701 ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg gggtggatat
     8761 ttttggcgag acttctactg tttcaaaggt aatctgatcg gcttcaatca caaaacagag
     8821 taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga gcgaatccgc
     8881 ccctttcacc acgtcagtta acgttgtcag atgtcccagt tcttcttcca gcaggtcgac
     8941 ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgactgc gactctgctc
     9001 atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg gcgtttcgcc
     9061 tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc tggcggcatc
     9121 cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct tcgtttccaa
     9181 aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt catcgagcag
     9241 gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc ctgcccctcc
     9301 tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg gcgcttccgg
     9361 ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt catcaaaaaa
     9421 tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg gctgatggca
     9481 ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt actggctcat
     9541 tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc agtaccagca
     9601 ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc atcgttgccg
     9661 ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc agttcgcggc
     9721 gggatgcatt ggcccgatcg gcagaaagct cccagttgct atatcctttc tcaccgctgg
     9781 cgtaggggaa atcatcggta tgacctgaaa ggctaatacg gttgggaata ccgttcagta
     9841 caggcgcaat gacacgcaga atgtcgcgca tatagggttc gacatcggca ctgccggttc
     9901 taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc tggaccagat
     9961 cgattttgag atggggacgt aacgcccgca gtttcggatc ggactctatc aactggtcga
    10021 gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcaga tcttcgatgt
    10081 tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg ccgggaattg
    10141 ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt ggagtccgga
    10201 agtactccgc aatctgaatc agctcttttg ggctggagat ggagatcagc cacatcacca
    10261 gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac gatccatgtg
    10321 ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc gcttgattct
    10381 tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca catgctcttc
    10441 cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa actcaacggc
    10501 gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc actgcatcat
    10561 tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa taaatccgta
    10621 agccaataaa atgccgagga aagtccccac catcgcatgt gcgataagcg cacccagctc
    10681 ggcggcagga cgatcggctg aacctaacgc gtgaacgccc cccattacag ccgcgacaat
    10741 accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga cttctgcctc
    10801 gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg tgttcatatg
    10861 accgctgata atcagacgca gataatcgac gataaaatca agcatgacgc tatccgcgag
    10921 gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat cacgttccag
    10981 cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag ccagcagatc
    11041 catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg ccttcagcgt
    11101 gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg caccggcaat
    11161 aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca tcaaataacc
    11221 gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga catcatcctt
    11281 ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat tggggcaaaa
    11341 aaagcagcgg tacgtcgtta ccgctgctgg aatgttgcgc ctcaccgtat cagttaaaca
    11401 gcctgtactc tctgttcatc cagcagttgt gggataatat cggcaggatt ctgggaaagt
    11461 ttacgtcttt ttactgcccg ggatggcggt tgacataagc tgcaggcaaa gctgccaaca
    11521 ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt tgcagctgga aagttgcagt
    11581 aatccacttt caacaaaccg caccaatgtc caggcacggg ttaatgccag cagtggtcct
    11641 tcttctgctt gtgggcactg ttcaaggtat aaacggtagg ctttgatcac cgcatcgacg
    11701 ccattacaca aaccggtttt cagtaaaaac tgccatgcat tacagaacat cgaagcatga
    11761 acgttttgtt cccaggtcat aaaccagtcg gttgagaatg gcagcatgcc tttcggcggt
    11821 gggcttccgc gcagttcttt ataaagtttt atcaggcgtc cgcgacttaa ctgtgtttcg
    11881 ctttccagca tctgcaaacg agcgcccagg gtaatcaatt ccattgccag ctgaatatcc
    11941 cgcgcttcct gaacaatgct tttttcactc atgatcaggc ccttttcttg cgcagcgctt
    12001 cttcaggctg attaacatca ttcagcaagc gtgttgagag catgatgccg gtatgaattt
    12061 gctggagatc gtcaacgcgg gaatcttgcg tcaactgagt aatcgtctgg tggctgtcaa
    12121 aacggaagtg acaaaccagt tgattggttt ctgccagctt aaccatttgc ggaagagtca
    12181 gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag acgaaacata gcggacgctt
    12241 tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga caagttgatg tcataaatgt
    12301 gtttcagcaa ctcggaggta tgcattattc ccacccagaa taaccaact
//
LOCUS       DAJVRF010000119        12028 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJVRF010000119 DAJVRF010000000
VERSION     DAJVRF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12028
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.119"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..324
                     /locus_tag="OQN13_003057"
     CDS             <1..324
                     /locus_tag="OQN13_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616346.1"
                     /translation="AASMLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLI
                     AIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATP
                     ASNVD"
     gene            458..1535
                     /gene="ygjJ"
                     /locus_tag="OQN13_003058"
                     /pseudo
     CDS             458..1535
                     /gene="ygjJ"
                     /locus_tag="OQN13_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            1552..3903
                     /gene="ygjK"
                     /locus_tag="OQN13_003059"
     CDS             1552..3903
                     /gene="ygjK"
                     /locus_tag="OQN13_003059"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCS1616347.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWLPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            4129..6147
                     /gene="fadH"
                     /locus_tag="OQN13_003060"
     CDS             4129..6147
                     /gene="fadH"
                     /locus_tag="OQN13_003060"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCS1616348.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(6192..6608)
                     /gene="higA"
                     /locus_tag="OQN13_003061"
     CDS             complement(6192..6608)
                     /gene="higA"
                     /locus_tag="OQN13_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCS1616349.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(6605..6919)
                     /gene="higB"
                     /locus_tag="OQN13_003062"
     CDS             complement(6605..6919)
                     /gene="higB"
                     /locus_tag="OQN13_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCS1616350.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(7204..8340)
                     /gene="rlmG"
                     /locus_tag="OQN13_003063"
     CDS             complement(7204..8340)
                     /gene="rlmG"
                     /locus_tag="OQN13_003063"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCS1616351.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            8425..8928
                     /locus_tag="OQN13_003064"
     CDS             8425..8928
                     /locus_tag="OQN13_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCS1616352.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            9005..9688
                     /locus_tag="OQN13_003065"
     CDS             9005..9688
                     /locus_tag="OQN13_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCS1616353.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            9767..10753
                     /locus_tag="OQN13_003066"
     CDS             9767..10753
                     /locus_tag="OQN13_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1616354.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            11036..12001
                     /gene="alx"
                     /locus_tag="OQN13_003067"
     CDS             11036..12001
                     /gene="alx"
                     /locus_tag="OQN13_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCS1616355.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         2858 a         3092 c         3225 g         2853 t
ORIGIN      
        1 gcagcgtcca tgcttccgcc gttattcatc atgctggctt acctgaattt acgcgccaaa
       61 ttagatcacc tgccacgcga tttccgtatg ggctcccgcc gcaccggtat tatcgttgtt
      121 tcaatgctga ttgcgatatt tgccgtaggg tttgtcgctt cgacattccc gactggcgcg
      181 aatattctga ccatcatttt ttataacgtc ggcggtattg ttatcttcct tggctttgcg
      241 tggtggaaat acagtaaata tataaaggga ttaacggctg aagagcgcca tattgaagcg
      301 acgccagcca gcaatgttga ttaagcagaa taataaaaaa tgagcaggca ttttccctct
      361 tcctggatga ggagagggaa aatagtttct gcctttatat ttttataatt acaacgataa
      421 aaggctgtac tttttcttta gctcatggat taacacaatg aaattaatca ctgcaccatg
      481 cagagcatta cttgctctgc cgttttgcta cgccttttct gcggcaggag aagaagcacg
      541 tccggcagaa catgacgaca caaaaacacc cgcaattacc tcgacatctt ctccttcatt
      601 tcgtttttac ggcgaattag ggattagggg gtggtggata tatggattta gagggtgaga
      661 ataaacataa atacagcgac ggtacctata ttgaaggtgg cctggagatg aagtacggct
      721 cctggttcgg cctgatttac ggcgaaggct ggaccgtgca ggccgaccac gacggcaatg
      781 cctgggtgcc agaccatagc tggggtggtt tcgagggcgg aattaaccgt ttctatggcg
      841 gttatcgtac caatgatggc accgaaatca tgctcagtct gcgtcaggat tcctcgctgg
      901 atgacctgca atggtggggc gatttcaccc ccgatctggg ctacgtcatt cccaataccc
      961 gcgacattat gactgcgctg aaggtacaga acttaagcgg caactttcgt tatagcgtca
     1021 ccgcgactcc tgccggacat catgatgaaa gcaaagcctg gctacatttt ggcaaatacg
     1081 atcgctatga cgacaaatac acctatccgg caatgatgaa cggttacatc cagtatgacc
     1141 ttgccgaagg catcacctgg atgaacggtc tggaaatcac cgacggcaca ggacagctct
     1201 atctcacggg cctgctaact cctaactttg ccgctcgcgc ctggcaccat accggacgcg
     1261 ccgacgggct ggacgtaccg ggaagtgaaa gtgggatgat ggtgagcgcc atgtatgaag
     1321 cgttaaaggg cgtttatctc tccaccgctt acacctacgc caaacatcgc cctgaccacg
     1381 ctgacgatga aaccacctct ttcatgcagt ttggtatctg gtacgaatac ggcggcggac
     1441 gtttcgccac ggcttttgat agccgcttct acatgaaaaa tgcctctcac gatcccagcg
     1501 accaaatctt cctgatgcaa tatttctact ggtaataagg actgtaattt catgaaaata
     1561 aaaactattt taacgccagt aacctgcgct ctgctgataa gtttttccgc ccatgccgct
     1621 aacgccgaca attataaaaa cgtgattaac cgtactggcg cgccgcagta catgaaggat
     1681 tacgattacg acgatcacca gcgttttaat ccgtttttcg atctcggagc ctggcatggt
     1741 catctgttgc cagacggccc taacaccatg ggcggctttc cgggcgttgc gctgctgacg
     1801 gaagagtaca tcaactttat ggccagcaat ttcgaccgcc tgaccgtctg gcaggacggc
     1861 aagaaagtcg actttacgct ggaggcatac agtattcccg gtgcgctggt gcaaaaactg
     1921 acagcaaaag atgtgcaagt cgaaatgact ctgcgcttcg ccacgccgcg cacgtcacta
     1981 ctggaaacca aaatcaccag caataaaccg ctggatctgg tgtgggacgg cgaactgctg
     2041 gaaaaactgg aagcgaaaga agggaaaccg ctttccgata aaaccattgc tggcgaatac
     2101 cctgactatc agcgcaaaat cagcgccacc cgtgatggcc tgaaagtcac ctttggcaaa
     2161 gtgcgcgcca cctgggatct gctgacctcc ggcgaatcgg aatatcaggt gcataaatcc
     2221 ctgccggtgc agactgaaat caacggtaat cgctttacca gtaaggcgca tatcaacggt
     2281 tcgaccacgc tctacaccac ctattcccat ctgctgaccg ctcaggaagt tagcaaagag
     2341 caaatgcaga tccgcgatat tctggcgcgt ccggcgtttt atctcaccgc ctcgcagcaa
     2401 cgctgggaag aatacctgaa gaaagggtta accaatccgg atgcgacgcc ggaacagacg
     2461 cgcgtcgcgg tgaaagcgat cgaaacgctc aacggtaact ggctctcacc gggcggtgcg
     2521 gtgaaattta acaccgttac accgtcggtg accgggcgct ggttctccgg caatcagacc
     2581 tggccgtggg atacctggaa gcaggcgttt gcgatggcgc atttcaatcc ggacatcgcc
     2641 aaagagaata tccgcgcggt cttctcctgg cagatccagc ctggcgatag cgtgcgtccg
     2701 caggatgtgg gctttgtccc cgacctgata gcgtggaacc ttagccccga gcgtggtggc
     2761 gatggcggca actggaacga acgtaatacc aaacccagcc ttgccgcctg gtcggtgatg
     2821 gaagtgtaca acgtcaccca ggataaagcc tggttggcag agatgtaccc gaaactggtg
     2881 gcctatcacg actggtggtt acgtaaccgc gatcacaacg gcaacggcgt gccggaatat
     2941 ggcgcgaccc gcgacaaagc ccacaacact gagagcggcg agatgctgtt tacggtgaaa
     3001 aaaggcgata aagaagagac gcagtctggg ctgaacaact acgcccgcgt ggtggagaaa
     3061 ggcctgtacg acagtctgga aattccggca caggttgctg catcgtggga atcggggcgt
     3121 gatgatgccg ccgtctttgg gtttatcgac aaagaacagc tggataaata tgtcgctaat
     3181 ggcggcaaac gtagcgactg gacggtgaaa ttcgccgaaa accgcagtca ggacggaacg
     3241 ttgctgggct actcgctatt gcaggagtcg gtggatcagg ccagctatat gtacagcgat
     3301 aaccattatc tggcggagat ggcaaccatc ctcggtaagc cagaagaggc caaacgctat
     3361 cgccagttgg cacagcagct cgcggactac atcaacacct gtatgttcga cccgactacc
     3421 cagttctact atgacgtacg tattgaagat aaaccactgg cgaacggctg cgcgggcaaa
     3481 ccgattgttg agcgcggtaa agggccggaa ggctggttgc cgctgtttaa cggtgcggca
     3541 acgcaggcca acgccgacgc ggtggtgaag gtgatgctcg atcctaaaga gttcaacacc
     3601 tttgtcccgc tgggaacggc ggcgttaacc aatccggctt ttggcgctga tatctactgg
     3661 cgtgggcgcg tatgggtgga tcagttctgg tttggtctga aagggatgga gcgttacggt
     3721 tatcgtgatg atgccctgaa gctggcggat acgttcttcc ggcacgccaa aggattaacc
     3781 gccgatggcc caattcagga gaattacaac ccgctgactg gcgcacagca aggcgcacca
     3841 aatttctcct ggagtgccgc tcatttgtat atgttgtata acgatttttt ccgtaagcag
     3901 taatttgcaa cggctggcgg atgcggcgtg aacgccttat ccgccctaca tgtgcaatcc
     3961 cccaggccgg ataaaacgcg gcaagcgttg catccggcgt taccaccggg cgtattcttt
     4021 ttgaatccca tcacaaaccc cgcactcccc ttttcccttt tctccatcga cggctaaatt
     4081 agaactcatc cgaccacata acaattattt tacatactgg acaattttat gagctatccg
     4141 tcgctgttcg ccccgctgga tttaggtttt accacgttaa aaaaccgcgt gttgatgggc
     4201 tcaatgcaca ccgggctgga ggaatacccg gacggtgccg agcggctggc agcgttttat
     4261 gccgaacgcg cccgtcacgg cgtggcgctg attgtcagcg gcggcattgc accagattta
     4321 acaggcgttg gcatggaagg tggcgcaatg ctcaacgacg ccagccagat cccacaccat
     4381 cgcaccatta ccgaagcggt acatcaggaa ggcggcaaaa tagccctgca aattttgcat
     4441 accgggcgct acagctacca accgcatctg gtcgctccct ccgcattgca ggcccccatc
     4501 aaccgtttcg ttcctcacga actcacccat gaagagatcc tgcaactgat cgacgatttc
     4561 gcccactgcg cgcaactggc acgggaggca ggatacgacg gtgtagaggt aatggggtcc
     4621 gaagggtatt tgatcaacga atttctgacg ctgcgcacca atcagcgtag tgaccagtgg
     4681 ggcggcgatt accgcaaccg gatgcgattt gccgtagaag tagtgcgtgc ggtgcgcgaa
     4741 cgcgtcggca acgacttcat tattatctac cgactgtcga tgctcgacct ggtcgaagac
     4801 ggcgggactt ttgccgaaac ggtagagctg gcgcaggcca ttgaagcggc gggcgcgacc
     4861 attatcaaca ccggcattgg ctggcatgaa gcacgtattc cgaccattgc cacgcccgtg
     4921 ccgcgcggcg catttagctg ggtcacgcgc aaattgaaag gccacgtctc gctgccgctg
     4981 gtaaccacca accggattaa cgatccgcag gttgccgacg atattctctc gcgcggcgat
     5041 gccgatatgg tatcgatggc acgaccgttt cttgctgatg cggaactgct gtcaaaagcg
     5101 caatcggggc gagccgatga gatcaacacc tgcattggct gcaatcaggc ctgcctcgat
     5161 cagatcttcg ttggcaaagt cacctcgtgc ctggtgaatc ctcgcgcctg ccacgaaacc
     5221 aaaatgccaa tccttcctgc cgtgcagaaa aaaaatctgg cggtggtcgg tgcgggacct
     5281 gctgggctgg cgtttgccat taacgcggcg gcgcgtgggc atcaggtaac attgtttgat
     5341 gcccacagcg agatcggcgg gcagtttaat atcgccaaac agatccccgg caaagaggag
     5401 ttttacgaaa cgctgcgcta ttaccaccgg atgatcgaag tgacgggcgt gacgctgaaa
     5461 ctcaatcaca ccgtgacggc ggatcagtta caggcgttcg atgaaacgat cctcgccagt
     5521 gggatcgtgc cgcgcgttcc gcctatcgac gggatcgatc atccgaaggt attgagttat
     5581 ctcgatgtac tgcgcgacaa agcgccggta ggcaacaagg ttgccatcat cggttgtggc
     5641 gggattggtt ttgatacggc gatgtattta agtcagccgg gcgaatccac cagccagaat
     5701 atcgccaggt tctgtaatga gtgggggatc gacagcagcc tacaacaggc tggtggctta
     5761 agcccgcagg gaatgcagat cccgcgtagc ccacggcaaa ttgtgatgct ccagcgcaaa
     5821 gccagcaaac cgggacaggg gttaggcaaa accaccggct ggatccatcg caccaccctg
     5881 ctctcgcggg gtgtgaaaat gatcccaggc gtaagttatc agaagattga cgatgacggg
     5941 ctgcatgtgg tgatcaacgg cgaaacgcag gtattagcag tggacaatgt ggtgatctgc
     6001 gcagggcaag agccaaaccg cgcgctggcg caaccgctga ttgatagcgg gaaaactgtg
     6061 catttaattg gcggctgcga tgtggctatg gagctggacg cacgacgggc aattgcccag
     6121 ggaacacggc tggcgctgga gatttaaatc gttattttaa gcgccggatg caacgcatcc
     6181 ggcacgtcgc gttaatcaat aaacaaggcg ggagaaatgc cgaatcgcgt tgccaatttt
     6241 ttagcgtgtt ccagcgttaa tttcctcttc ccgctcaaaa cgcgtgacac catagattta
     6301 ctgccaattt ccggcagatc ggcaagggtt aaaccatatt gatccataag ggtacgaatc
     6361 acggctatac cgccaggcat ggcttgagcc atggcattaa attccgcaaa ttcgggcgct
     6421 gattcttccc acgcggttat tttggcacac accagatcca gcaaggggtt ttcaggatcg
     6481 ttgagcagca gatgatctac cagttccagc gcctgggtgt attgttcctc gttctgaata
     6541 cccgccagaa aaggtgccac cgcagttagc ttttctcctg cttgcaagat gtcggcaatc
     6601 gcaatcattt tttcccctta gtacgatgaa cagcggtaaa gaaatcgtat tctttatgcg
     6661 tcataacttc acgtatgtag cacttttgcg attcaaaaaa gaccattgct acaacacgta
     6721 attcattgcc cccaacattg aaaacataat gcttatccag atatttgaag ttatccagag
     6781 atgggaatac tgcttttaat gactcaggtt ttttgaaata tcccttagca atcgtgttcc
     6841 ccagagccac caactccgtt ttatgttgcg ggtatttttc cgcagcatct ttcaatgctt
     6901 tttgagttat caggtgcatt cttcatcacg tccgttgaca aattggcaat atgataacat
     6961 ccgttgacag attggcaacg gatgaattta tctgtgtgac ggaagaataa acgtagaaaa
     7021 acgctaaaca agagaatgta gaggatggtg agagggattt tttgttttat ggaagcgcga
     7081 ttccagaact gggcagacat tgccggatgc gacgcttgcc gcgtcttatc cggcctggga
     7141 gtaacgtgct gaacgtaggc cggataaggc gttaacgccg catccggcag gtaactccca
     7201 atcttaccga cgacgcccca gcttcactgc tttcagcacc acaaatttat tattcgtggc
     7261 gatggtggtg cagttgccga aaatcttctt cagtttatgg aagtaatcca ggtgacggtt
     7321 ggcaacaata tacagctcgc cgttgatttt caggcagcgg cgggcatggt ggaacatctc
     7381 ccaggcgacg ttatcggtca gcgcatgttg ttggtgaaac ggcgggttgc agagcacagc
     7441 attaaagcgg aaaggctcca cgccggagag cgcgttgttg atcataaact cgctgcgatc
     7501 taacgcctct ggcatgttgg tttcaacgtt caaacggctg gaagcaaccg ccatcggcga
     7561 ttcatcgaca aacaccactt tcgcctgagg gtttttatca agcagcgtca gaccaataac
     7621 gccattaccg caaccgagat cgacaatctc accttcgaga ttctctggca gatgttgcat
     7681 aaagaagcgt gcgccaatat ccagcccggt gcgggagaag acattcgcat ggttgtggat
     7741 agtccagtca gtaccttcca gcttccagct aacggtctgc ggcgcatcgg ccagcggcgg
     7801 ttcattgaaa gtgcaattaa tcaggcgcgc ttttttccat gccagcgtag tggtggtcgg
     7861 gccgagcact ttctcgaaca gctccagcgt ggaagtgtga atgtcacggg ctttggcacc
     7921 ggcaataata cgtgtatccg acgtgaccac tttgcgcagc gcacgcagtt gctgttccag
     7981 caatgccagt gttttcggca ctttgatcag caccacaccc ggctgttgcg ggtagtcggc
     8041 ggtgctgtcg agaaacttca cgctcgactc atcaatcccg ttgaggcgta aattctcgcg
     8101 cgtcgccagt tcactgatgt atgagtcgcc aatgctgtac ggcttatgtt ccgccagcgc
     8161 gcagcttaac gcaccaaagg catcattcag gatcaacacc gggccgcgga tttctgtgtc
     8221 gtccaactgt tgcagcaaat attcatccgc cgcttcccac gcctgtagcg ggttaacgtc
     8281 atccgtcgcc ggaaaacgtt gtagtgtcag tgaacggaaa ccgttgtcta agtggctcat
     8341 cggccctcct gaatgataaa atttcgacgt tgcccctgaa aagggtgcgt gagtataccc
     8401 tttttctctt tttcgtgggc ggttatgagc aatcttactt atcttcaggg ttatcccgag
     8461 cagctacttt cccaggtgcg aacgctgatt aacgaacagc gtctgggcga tgtgctggca
     8521 aaacgctatc cgggaacaca cgactacgcc accgataaag ccctctggca atatacccag
     8581 gatctaaaaa atcagtttct gcgcaatgcc ccgccgatca ctaaagtgat gtatgacaat
     8641 aagatccacg tactgaaaaa cgcgctcgga ttacataccg ctgtttctcg tgtgcagggc
     8701 ggcaagctga aagcaaaagc agagatccgc gtcgctactg tgtttcgcaa cgcgccggaa
     8761 ccgtttttac gcatgatcgt ggtgcacgaa ctggcgcacc tgaaggagaa agagcataac
     8821 aaagcgtttt accagttgtg ctgccatatg gagccgcagt accaccagct tgagttcgac
     8881 acccggctgt ggctaacgca gttgtcgctt gggcaggaca aaatctgaaa atgatgtcag
     8941 gcgttatctt ttctttcttt agcgcctaaa atcgacctcc cccctttcgt taaggataac
     9001 gaccatgcag ccccgccttc ttctccgtat ttgtttttct cgccggactc taaagattac
     9061 ctgcttactg ttacttgtgg caggagcgac catttttatc gccgatcgcg taatggtcaa
     9121 cgccagcaag cagctgacct ggagcgatgt caacgcagtt ccggcgcgta acgtgggttt
     9181 attgctgggg gcgaggccgg gtaatcgcta ctttacccga cgtattgata ccgcagcagc
     9241 gttgtaccac gtcggaaaag tgaaatggct gttagtcagt ggcgataacg gacgtaaaaa
     9301 ttacgatgaa gcctcaggta tgcagcaggc tttgatcgct aaaggcgtac cggcgaaagt
     9361 gatcttctgc gactatgccg gattctcaac gcttgattca gtggtgcgtg ccaaaaaggt
     9421 gtttggggaa aaccatatca ccattatctc gcaagaattt cataaccaac gcgccatctg
     9481 gctggcaaaa cagtacggca tcgacgctat cgggtttaac gcgccagacc ttaatatgaa
     9541 gcacggtttt tatactcaac tgcgggagaa actcgctcgt gtcagcgcgg tgattgacgc
     9601 caaaatcctc catcgccagc cgaaatatct cgggccatcg gtcatgattg ggccgtttag
     9661 tgagcatggc tgtccggcga aagaataatg cgtatctgcg cacgtcgaag atgaaaaagg
     9721 cgtgctacat tgacgacaga atccctttat ggagtatcca cgcgttatga tacgtttcgc
     9781 tgtgattggt acgaactgga tcactcgcca gttcgtcgag gccgcccatg agagcggtaa
     9841 atacaagtta accgccgtat attcccgcag ccttgaacag gcccagcact tcgccaatga
     9901 tttttctgtc gagcatctgt ttacctcgct ggaagcgatg gcggaaagcg atgccattga
     9961 cgcggtgtat attgccagcc cgaatgccct gcacttttcc cagacacaac ttttccttag
    10021 ccataaaatt catgtgattt gcgagaaacc actggcgtcg aatctggcgg aagtggatgc
    10081 cgccattgcc tgtgcgcggg aaaatcaggt ggtgctgttt gaggcattta aaaccgccag
    10141 cctgccgaac tttcatttgc tgcgccaggc gctgccgaaa gtcggcaaat tgcgtaaagt
    10201 ctttttcaac tattgccagt attcctcgcg gtatcaacgt tacctggatg gtgagaatcc
    10261 caacaccttt aatccggcat tctctaacgg ttcaattatg gatatcggct tttactgcct
    10321 ggcgtcggcg gtggcattat tcggtgaacc gaaaagcgtg caggcaaccg ccagtttgtt
    10381 ggcaagcggc gtagatgccc acggcgtggt ggtgatggat tacggtgatt tcagcgttac
    10441 gttgcagcac tcaaaggtca gtgattctgt cctggaaagc gagattcagg gcgaagcggg
    10501 atcgctggtg attgaaaaac tgtctgaatg ccagaaagtg tgcttcgtgc cgcgtggcag
    10561 ccagatgcag gatctcaccc agccgcagca tattaatacc atgctctacg aagcagagct
    10621 gtttgctacc ctggtggatg agcatctggt ggatcatcca gggctggcgg tcagtcgcat
    10681 caccgccaaa ctgctgactg agatccgccg ccagactggg gtgatttttc cggcagatag
    10741 cgtaaaacta taattgccaa agtaaaacag tgtaaaaggt atgtaacaga ccattgactg
    10801 gctgaatggt ctgtcatact ttgttacctg caaaggggag taacttcatt gccggtcgat
    10861 cgtcattacg atgtgtgaaa aaccacatcc ggtcaccggg caacccgaaa ggaatacgca
    10921 gacgtattcc ttttttgttg taagtgagac cttgccggaa ggcgaggtct atgcataaaa
    10981 agcagcggct gacgtcttcc gacgttggcc gtttttttat gtgtaaggaa cttctatgaa
    11041 tactgtcggc acgccgttgc tatggggcgg attcgctgtt gttgtcgcca ttatgctggc
    11101 tatcgacctg ttgttgcagg ggcgtcgtgg ggcacatgcc atgaccatga aacaggctgc
    11161 ggcctggtcg ctggtctggg tgacgctgtc gttactgttt aacgccgctt tctggtggta
    11221 tctggtgcaa actgaaggtc gcgccgttgc cgatccacag gcactggcct ttctcacggg
    11281 ttatctgatt gagaaatcgc tggcggtcga taacgtcttt gtctggctga tgttgttcag
    11341 ctatttctct gttccggcgg cattacaacg ccgcgtgctg gtgtatggcg tgctcggggc
    11401 gattgttctg cgtaccatca tgatcttcac tggcagctgg ctaatttcgc agttcgactg
    11461 gatcctgtat atcttcggtg ccttcctgct gtttaccggc gtgaagatgg cgctcgccca
    11521 tgaagatgaa tcaggcattg gcgacaagcc gctggtgcgc tggctacgcg gtcatttgcg
    11581 catgaccgac accatcgaca acgagcattt ctttgtgcgt aagaatggat tgctgtacgc
    11641 cacaccgctg atgctggtac tgattctggt ggaattgagc gacgtgattt tcgccgtgga
    11701 tagcattccg gctatcttcg ccgtgaccac tgacccgttc attgtgctga cctcaaacct
    11761 gtttgcgatc ctcggcctgc gtgcgatgta tttcctgctg gcgggcgtag cagagcgttt
    11821 ctcgatgctc aaatatggcc tggcggtgat tctggtgttt atcggtatca agatgctgat
    11881 tgtcgacttc taccatattc caatcgccgt ctcgctgggc gtggtgtttg gcattctggt
    11941 gatgacgttt attatcaacg cctgggtgaa ttatcggcat gataagcagc ggggagaatg
    12001 attcgctgta tattaagcca ttcctaga
//
LOCUS       DAJVRF010000120        11877 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJVRF010000120 DAJVRF010000000
VERSION     DAJVRF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.120"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            486..1157
                     /locus_tag="OQN13_003068"
     CDS             486..1157
                     /locus_tag="OQN13_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HCS1616356.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCNQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            1167..2363
                     /locus_tag="OQN13_003069"
                     /pseudo
     CDS             1167..2363
                     /locus_tag="OQN13_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            2363..2923
                     /locus_tag="OQN13_003070"
     CDS             2363..2923
                     /locus_tag="OQN13_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCS1616357.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2925..3698
                     /locus_tag="OQN13_003071"
     CDS             2925..3698
                     /locus_tag="OQN13_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HCS1616358.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3886..4161
                     /gene="frmR"
                     /locus_tag="OQN13_003072"
     CDS             3886..4161
                     /gene="frmR"
                     /locus_tag="OQN13_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HCS1616359.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            4196..5305
                     /gene="frmA"
                     /locus_tag="OQN13_003073"
     CDS             4196..5305
                     /gene="frmA"
                     /locus_tag="OQN13_003073"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HCS1616360.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5398..6231
                     /gene="fghA"
                     /locus_tag="OQN13_003074"
     CDS             5398..6231
                     /gene="fghA"
                     /locus_tag="OQN13_003074"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCS1616361.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6457..6996)
                     /locus_tag="OQN13_003075"
     CDS             complement(6457..6996)
                     /locus_tag="OQN13_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HCS1616362.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(7098..8309)
                     /gene="mhpT"
                     /locus_tag="OQN13_003076"
     CDS             complement(7098..8309)
                     /gene="mhpT"
                     /locus_tag="OQN13_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HCS1616363.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALALLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8596..9609)
                     /gene="dmpG"
                     /locus_tag="OQN13_003077"
     CDS             complement(8596..9609)
                     /gene="dmpG"
                     /locus_tag="OQN13_003077"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HCS1616364.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9606..10556)
                     /gene="mhpF"
                     /locus_tag="OQN13_003078"
     CDS             complement(9606..10556)
                     /gene="mhpF"
                     /locus_tag="OQN13_003078"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HCS1616365.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10553..11362)
                     /gene="mhpD"
                     /locus_tag="OQN13_003079"
     CDS             complement(10553..11362)
                     /gene="mhpD"
                     /locus_tag="OQN13_003079"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HCS1616366.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDGLYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11372..>11877)
                     /gene="mhpC"
                     /locus_tag="OQN13_003080"
     CDS             complement(11372..>11877)
                     /gene="mhpC"
                     /locus_tag="OQN13_003080"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HCS1616367.1"
                     /translation="AFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQLYRQP
                     TIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFPDFGPR
                     LAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADAFNQLV
                     LNFLARP"
BASE COUNT         2912 a         3178 c         2908 g         2879 t
ORIGIN      
        1 aatatgcaat tactccgttc taacaattgg gaaaattact acacttcatt ttagtgatgc
       61 ataaacaacc acattacgag atttattact tcattgtttc tatcaataca tataataaaa
      121 ctattatatt cgcattgtat atatcactgt atcattctca atcactcgca tattacattc
      181 acataatgta tataaacaat acttgttcac agaacatatt ttttaaatgc aattaattga
      241 caaactaaat cacatctaaa tacaatgtat aaaacaaaat aactatacat accaaacata
      301 caaacactta acatatgaat tgcataaaaa cattacccag tcgaacctcc gggtctgaaa
      361 gcccttgctt ttcagttatt agcaacattt aacatttata aattaacatc tggctgcgcc
      421 agagattacg tctctgagtg catcgttgtg cttgtagtat aataaataaa atggagtgtc
      481 attaagtgga taaggtttta gattcagccc tcctttcttc ggcaaataaa agaaagggta
      541 tccttgctat tggcgcacat cctgacgata tagaattagg ctgtggcgca tcgcttgctc
      601 gtcttgcgca aaaaggaatt tatatcgcag cagtggtgat gactaccggc aactctggta
      661 cagatggaat aatcgatcgc catgaagaat cgcgcaacgc cctaaagata ttagggtgca
      721 accaaactat tcatcttaat tttgctgaca cccgcgctca tttacagctc aatgatatga
      781 tttccgccct ggaagacatc attaaaaatc aaattccttc tgatgttgaa atcatgcggg
      841 tatataccat gcatgatgcc gaccgccatc aggatcatct cgctgtttat caagcttcaa
      901 tggttgcctg ccgcactatt ccacaaattc tcggctacga aaccccaagt acctggcttt
      961 catttatgcc tcaggttttt gaatccgtta aagaagaata tttcacagtc aaacttgctg
     1021 cattaaaaaa acataaaagc caggaacgac gtgattatat gcgtcatgat cgcctgcgtg
     1081 cagttgcaca atttcgcggg caacaggtca atagcgatct gggtgaaggc tttgtaattc
     1141 ataaaatgat tctttgagca aattgcatga aaacctggat atttatctgt atgtccatag
     1201 caatgttgct atggttttta agtacgctaa gacgtaaacc cagtcaaaag aaaggctgta
     1261 ttgacgccat tatacctgcg tataacgaag gcccgtgtct ggcgcagtca ctggataatc
     1321 tactgcgtaa cccttatttt tgccgggtaa tttgcgttaa cgacggctcc acggacaata
     1381 ccgaagcggt catggcggaa gtcaaacgca aatggggcga ccgctttgtt gccgtcacgt
     1441 aaaaaaatac cggtaaaggt ggtgcgctga tgaatggcct caattatgcc acctgcgacc
     1501 aggttttttt aagtgatgcc gacacctatg ttccgcccga tcaagacgga atgggctata
     1561 tgctggcaga aattaagcgc ggtgctgatg ccgtaggcgg cattccctct actgcgttga
     1621 aaggcgcggg gctgttaccg cacatccgcg cgaccgtaaa gttgccgatg attgttatga
     1681 agcgcacgct acagcagctc ctgggcggcg caccgtttat tatcagcggt gcctgcggaa
     1741 tgttccgtac tgatgtattg cgtaagttcg gtttctcgga tcgtactaaa gtcgaagacc
     1801 ttgatctcac ctggacatta gtggcaaacg gctaccgtat tcggcaggcg aatcgctgca
     1861 tcgtataccc acaggaatgc aacagtccgc gtgaggagtg gcgtcgctgg cggcgttgga
     1921 ttgtgggcta cgcggtctgt atgcgcctgc ataaaaggct tttatttagc cgcttcggta
     1981 tcttcagtat atttcctatg ctgttggttg tgatttatgg cgttgggatt tatctcacta
     2041 cctggtttaa tgaattcatc accaccgggc cgcatggtgt ggtgttggca atgtttccgc
     2101 ttatctggat cggcgtagtt tgtgttattg gtgcttttag cgcctggttt catcgttgct
     2161 ggttgttggt gcctttagcg ccgctttccg ttgtgtatgt attattagct tatgccatct
     2221 ggattatcta tggacttatt gcctttttta ctggacgcga acctcagcgc gacaaaccca
     2281 cccgctattc cgcactggtg gaagcgtcaa ccgcttattc ccaaccttct gtcacaggaa
     2341 ctgaaaaact atctgaagct taatgttaaa gagaaaaatt attcagattg cagaccaggt
     2401 aattattgat gaaactgcag gtgaagttgt tatcggcgcg aatacccgta tttgtcatgg
     2461 tgccgttatt cagggtccgg tagtgattgg cgcaaactgc ctgataggta attatgcgtt
     2521 tattcgtcct ggcacaataa tcagcaatgg tgtaaaaatt ggttttgcca ccgaaattaa
     2581 aaatgcggtt attgaagcgg aagcaacgat tggtccgcaa tgttatattg ccgactcggt
     2641 agttgcaaac caggcatatt tgggcgcaca agtacgtacc agtaatcatc gtctggatga
     2701 acagcctgtg tctgttcgaa ctccagaggg aattatcgct accggatgcg ataaattagg
     2761 ttgttatatc gggcagcgtt cacgccttgg cgtacaagtt attattttgc ctgggcgaat
     2821 tatttccccg aacacgcaac ttggcccgcg cgtgattgta gaacgtaatt tacctaccgg
     2881 aacttattca ctccgacaag aacttatccg tacaggagat taaaatgata aaacggacgt
     2941 tattagcggc ggccattttt agcgcattgc ccgcttatgc cgggttaact tccattaccg
     3001 cgggctacga ttttaccgat tattctggcg atcatggcaa ccgtaattta gcgtatgctg
     3061 aactggtggc gaaagttgaa aacgcaacgc tgctttttaa tctttcccag gggcgtcgtg
     3121 attatgaaac tgaacatttc aatgccactc gcggtcaggg tgcggtctgg tataaatgga
     3181 ataactggct gacaacccga acgggtattg cctttgcgga taatacgccg gtctttgccc
     3241 gccaggattt tcgtcaggat attaacctgg ccctgttgcc aaaaacgctt ttcacgaccg
     3301 gttatcgcta cactaaatat tacgatgatg tcgaagtcga tgcctggcaa ggcggcgtat
     3361 cactctatac tggcccggtg atcaccagct accgctatac ccattatgac tccagcgatg
     3421 caggtggtag ttatagcaat atgatttccg tgcgtctgaa tgacccgcgc ggcactggtt
     3481 atacgcaact atggctaagc cgcggaacag gcgcttacac ctatgactgg acgccagaaa
     3541 cacgctacgg cagcatgaag agcgtcagtt tgcaacgtat tcaaccgctg actgagcaac
     3601 ttaatctcgg gctgacggca ggtaaagtgt ggtacgacac cccaaccgat gattataacg
     3661 gtctgcaact tgcagcccat ctgacctgga aattctgatt gcttctgccg cccgctatcc
     3721 ggggcggcct tccctgccga ttagccccac tccctttcct cgttgttttc cgaccacatt
     3781 caccggataa attttattct ccagcgttat atactatagg gaggtatgca ttgacatata
     3841 gcatacctcc ctatagtata ttgcgtgcag atgatgaggt gcgaaatgcc cagtactccg
     3901 gaagagaaga aaaaggtcct tactcgagtt cgtcgtattc gggggcagat tgatgctctg
     3961 gaacggtcgc tggagggtga tgccgaatgc cgtgccatac tccaacagat cgctgccgtt
     4021 cggggcgcgg ctaacgggct gatggcagaa gtgcttgaaa gccatatccg ggaaacgttt
     4081 gaccgaaatg actgctacag ccgcgaagtc agccaatccg ttgacgacac tattgaactg
     4141 gttcgagcct atcttaaata gctgaatcta ttaccatatt gaggaagagc gagagatgaa
     4201 atcacgtgct gccgttgcat ttgctcccgg taaaccgctg gaaatcgttg aaattgacgt
     4261 tgcaccaccg aaaaaaggtg aagtgctgat taaagtcacc cataccggcg tttgccatac
     4321 cgacgcattt accctctccg gggatgaccc ggaaggtgta ttcccggtgg ttctcggtca
     4381 cgaaggggcc ggcgttgtgg ttgaagtcgg tgaaggcgta accagcgtca aacctggcga
     4441 ccatgtgatc ccgctttaca ccgcggagtg cggcgagtgt gagttctgtc gttctggcaa
     4501 aactaacctc tgtgttgcgg ttcgcgaaac ccagggtaaa ggcttgatgc cagacggcac
     4561 cacccgtttt tcttacaacg ggcagccgct ttatcactac atggggtgct ctacattcag
     4621 tgaatacacc gtggtcgcgg aagtgtctct ggccaaaatt aatccagaag caaaccatga
     4681 acacgtctgc ctgctgggct gtggcgtgac caccggtatt ggcgcggtgc acaacacagc
     4741 taaagtccag ccaggtgatt ctgttgccgt gtttggtctt ggcgcgattg gtctggcagt
     4801 ggttcagggc gcgcgtcagg cgaaagcggg acggattatc gctatcgata ccaacccgaa
     4861 gaaattcgat ctggcgcgtc gcttcggtgc taccgactgc attaacccga atgactacga
     4921 caaaccgatt aaagatgtcc tgctggatat caacaaatgg ggtatcgacc atacctttga
     4981 atgcatcggt aacgtcaacg tgatgcgtgc ggcgctggaa agtgcgcacc gcggctgggg
     5041 tcagtcggtg atcatcgggg tggcaggttc tggtcaggaa atctccaccc gtccattcca
     5101 gttggtcact ggtcgcgtat ggaaaggttc cgcgtttggc ggcgtgaaag gtcgttccca
     5161 gttaccgggt atggttgaag atgcgatgaa aggtgatatc gatctggaac cgtttgtcac
     5221 gcataccatg agccttgatg aaattaatga cgccttcgac ctgatgcatg aaggcaaatc
     5281 cattcgaacc gtaattcgtt actgatttcc cgcaggtata ccccgtccac ttcagacggg
     5341 gttcttaata ctctccccgg gcagccgtcc gggggattaa ccgtgagata atgactgatg
     5401 gaactcattg aaaaacatgc cagctttggc ggctggcaaa atgtgtatcg gcattattcc
     5461 caatcactga aatgtgaaat gaatgtcggc gtctatctcc caccaaaagc cgcgaatgaa
     5521 aaattgccgg tgctgtactg gctttcaggc ctgacctgca acgagcagaa tttcattact
     5581 aaatcgggga tgcagcgtta cgcggctgag cacaacatta ttgttgttgc gccggacacc
     5641 agtccgcgag gcagtcatgt cgcagatgcc gaccgttacg atctcgggca aggtgccggg
     5701 ttttacctga acgcaacgca agcgccgtgg aatgaacatt acaaaatgta tgactatatc
     5761 cgcaacgagc tgccggattt agtgatgcaa cattttccgg caacgaccag aaagtctatc
     5821 tctggtcatt ctatgggcgg gctgggcgcg ctggtgctgg cgttacgtaa cccagatgaa
     5881 tatgtcagcg tctcggcgtt ttcgcccatt gtctccccat cgcaagtacc gtggggacag
     5941 caagcctttg ccgcatatct tggtgaaaat aaagatgcct ggttggatta cgatccggtg
     6001 agtcttattt cacaaggtca acgcgttgcg gaaatcatgg ttgatcaggg gttgagtgat
     6061 gatttttacg cggaacagct gcggactcca aatcttgaaa agatctgcca ggagatgaat
     6121 atcaagacgt taatccgtta tcacgagggt tacgaccaca gctattattt tgtctccagt
     6181 tttattggcg agcatattgc ctaccacgcc aataaactga atatgcgttg ataatagtgc
     6241 acgactgccg gatgcggcgt gaacgcctta tccggcctac acttcgcccg taaaccgtag
     6301 gcctgataag acgcgcaaag cgtcgcatca ggcatggcgc acgactgccg gatgcggcgt
     6361 gaacgcctta tccggtctac acttcgccca taaaccgtag gcctgataag acgcgcatag
     6421 cgtcgcatca ggcatctgcg cacgacttat ctatgtttac cacatcaaat catcaggcac
     6481 tttgaagtcg gcatacggat cgtcttcatc ctgctcttcg gcactgagcg cgctgtgcaa
     6541 cacaatactg ctggcatcac gctgagcaat tttatcggca acactggcgg gaataatggt
     6601 gtattcacct tcgctattgt tatcgaccaa caagcgggca atcgccagac gaccattaat
     6661 caactgcgcc tgtgtgagct tatcgacaaa aatcttctta atcagattgc cgtcagtgaa
     6721 gttaaaacca atatcgccat tggcaatggt gattcggttc atttcaatga gctgcttcac
     6781 ctgggcttta tattctttcg ccaacgccgc ttgtttttgc tgttcgctaa gctgtttatc
     6841 acgctcaagc tgtgcctttt tattttcttc taccgcagcc cgagcttcac gcgcctgaac
     6901 gcgcgatttt ttcgccgttc tctcgacctt cgccgctttt ttgctggtga ctaatcctgc
     6961 tttgagcaac tgctcttgta aggtaagttt tgccatcttt gtttccaaac ccgctgaata
     7021 atttttagcg agtatacctg taaacgccgt gggtgtattg ggggcaagtc cctttcgccc
     7081 ctgcttaata aggcgcgtca ggcatcggca cacggctgca ttcttgatct ccgactcatc
     7141 aaaataaaca ccgccaaccc agcaacaaga atacccggtg cagaagcggc cattacgccg
     7201 accgtgccag tgcctaatgc cagcattttc ccggccagta acggaccgct catagccccc
     7261 agacgcccta ccgccacggc tgttcccaca cctgttgcgc ggatctgcga actgtaaaac
     7321 aacagtgcca gggcatacaa aacgctttgc ccacctgtcg caaacaaccc cgcgacaaat
     7381 cccgccagca acataccgtt aaatgacgac accgttccaa gcgccagcag cgaagctaac
     7441 atgccgctat aaatcagtag cgacatggtt actggacgca gcttatccat caatgcgccc
     7501 aacattaacg tcccgcttgc cgcccccatt tgcagggcaa acatcacccc tgccgcctgc
     7561 gatggctgga atccttgctc caccaaaagt agcggtagcc agttgatcaa catataaacc
     7621 accagcagag tgaagaaata acacaaccac agcagcagcg tcgcggttgc cgtttctggc
     7681 gcaaataagg cacgcagtgg tggcgcagcc tgtttttcgc cagcgaaaac cgctgactcc
     7741 ggcagccagc gcattaatag cggcaccaga atcaacggca ccacaccacc tacccaaaac
     7801 accgtttgcc atgctaagtt tgcccccgcg aaacccagtg tcgccgccag cgccgcgcca
     7861 atgggaacac cgcaatacat caggctcact gccgtcccac gaaaacgtgg acccgcggct
     7921 tcagatgtca gggcgataag attcggcaac gccgccccca gcccgacacc ggtcatcagc
     7981 cgcgcaaaga ccagtgaggg gaaatcccag gcaatcgccg ttgccagtga gaacaaacca
     8041 aacagcgcaa ctgagccaat caaaatacgc ttacgaccat aacggtctgc cagcatcccg
     8101 ccaaccaacg cgccgggtag caaaccgagt attccggcgc taaatatcca gcccatttgc
     8161 attttatcga gtgcgaaagc ctgggcgatg ccacccgccg caatgccagc cgcctgaaga
     8221 tccagccctt ccatcagagc gaccaaaaaa caaagcccga tggtcagcat caggcgggat
     8281 gaagatgatg aaggggtacg agtcgacata agatgaacct cattgcgagt gcaggtgaat
     8341 cgcgactgat acattgccag ccgcgtaatc agaactattt aatgactgcc tacgggcgaa
     8401 atgtagcgaa atgtaggccg gataaggcgt tcacgccgca tccggcagtc gtgcgcagat
     8461 gcctgatgcg acgctaacgc gtcttatcag acctacaaat tctcacgcca tccgtaggcc
     8521 ggacaaggcg ttcacgccgc atccggctgt cgtgctggga tgggcataca aaatgcacgt
     8581 tatcggcagt catcattatt tgttgttgcg cagatccagc gccacgtcga cgatcatatc
     8641 ctcctggccg ccaaccatcc ggcgtttgcc cagctcaacg agaatatcca ccgcacttaa
     8701 gccataacgc gccgccgccg tttcacagtg acgcaggaag ctcgagtaaa caccagcgta
     8761 tcccagcgcc agcgtttcgc gatcgactcg taccggacga tcctgcaacg gacgcaccag
     8821 gtcgtcggcg gcatccatta acgcatagag atcggtccca tgctgccagc ccagtttatc
     8881 cgcggcggca ataaacactt ccagcggtgc gttacctgcg cccgcgccca ttcccgcgag
     8941 gctggcgtcg attcggtcgc agccctcttc caccgccgcg atagagttcg ccacgccaag
     9001 actcaggtta tggtgagcgt gcatgccggt ttgcgtttct ggtttcaaca ctgctttcag
     9061 ggcgcggaaa cggtcacgga tatcgctcat gttcatcgca ccgccagaat ccaccacata
     9121 aatacaggtc gcaccgtagc cttccatcag ctttgcctgc ttggcgagat tctccggcgt
     9181 ggtcatatgg ctcatcatca gaaaaccaac ggtgtccatt ccgagctcgc gggcatactg
     9241 aatatgctgg gcggaaacat cagcttcggt acagtgcgtt gccacacgaa ccacccgcgc
     9301 gccagcctgc caggcatttt tcagatcgtg aatagtgccg attcctggca gcaacaacgt
     9361 cgcgattttg gcgtgcctca ccacatccgc cgccgcttca atccattcaa ggtcgctatg
     9421 tgcgccgaaa ccatagttaa agctggaacc ttgcaaaccg tcgccgtggg ccacttcaat
     9481 cgaatccacg cgggcatcgt ccagtgcttt ggcaatctgg cgaacgtttt ccagcgaata
     9541 ctgatgacga atggcgtgca taccgtcacg caatgtgacg tccgagatat aaagtttttt
     9601 accgttcatg ccgcttctcc tgccttgcgc gccagtgact gggccatttt ttccgctgtc
     9661 gccagcgcac tggaagtcat aatatcgagg ttgcccgcat aggcaggcag ataatgcgct
     9721 gcgccttcga cttccagcca gaccgctgtt ttcagtccgg agaattgccc cacgcccggt
     9781 aaattgaccg gtttatcctg cgggataact tcaaactgca cgcgctgttt caggcgataa
     9841 cccggtacgt aagcctgcac cgcctcagcc atttcattga ttgaggcttc gatatcatct
     9901 tgtgaagctt cgtcgctcaa tacatacacc gtgtcacgca tcatcaacgg tggctctgct
     9961 gggttaagca caataatcgc cttccctttt gccgcgccgc ccaccacttc aatggctcgg
    10021 gaagtggttt ccgtaaattc atcgatattg gcacgcgtgc caggtccggc agatttactg
    10081 gcgatagaag cgataatttc ggcgtaatga acacgcgcca cgcgtgaaac tgccgccacc
    10141 attggaatgg tggcctggcc gccgcaggtg accatgttga cgttcagttg atcgacgttc
    10201 gcctcgaggt taaccaccgg cacgcagtaa gggccgatgg cagcaggcgt caggtcaatt
    10261 aagcgaatat ccggtttcgc ttcgcgtaaa gcggcatcgt ttttcacatg cgcgcccgcg
    10321 ctggtcgcat caaatacaat gtcgatatca gcaaattcag gcatgttcat cagcccgatc
    10381 accccttcat gggtggtggc gacgcccata cgtctggcac gcgccagacc gtcggactga
    10441 ggatcaatgc cgaccatcac cgccatctcc agatgctgac cgtgacgcaa aattttaatc
    10501 atcagatcgg tgccaatgtt gccagaaccg ataatggcga ctttacgctt actcatgaca
    10561 gacttccttt tggggctgcg cttgaaaatg tcgcagcaac tgaacctatg ccttcaatat
    10621 gggcttcaaa acgatcgccc gcattcaccg ccaccatcgg gcctaatgcc ccggtaagaa
    10681 tgatatctcc ggcgcgcagc ggttcaccca gactggccat tttgcgtgcc agccagacgg
    10741 cggcattaag cggatgtccc aggcattcgc tgccgcgccc gctggaaacc tcttcgttat
    10801 tacgcgtcat cttcatggcg cagtttttca ggtctaaccc cgccggacgt tgcgccggac
    10861 cgccgatgac atacaccccg caggaagcgt tatctgccac ggtatcgaca aactgaatcg
    10921 accagtcgcg aatgcggctc cccaccactt ccagcgccgg aagtacccat tcaatggcgt
    10981 tatacagtcc gtcgaaggtg atatcagttg cgggtaaatc gcggttcaac accagtgcga
    11041 tctccgcttc aatgcggggt tgcagaacac gggaaaaagg aatgatttcg ttatcgccat
    11101 aacacatgtc ggcaaataac gtcccaaaat ccggttgatc aacgcccagt tgttgttgca
    11161 ctttcggatg cgtcaggccc actttacgcc ctaccacgcg acgcccctgc acaacgtcat
    11221 attgcacatt tatgtgctga atggcgtagg ccgcttcagc gtgatcgata ccaatcagat
    11281 cgcgcagcgg cgcaatcgct tcgccctgct ctgcggcgcg gcgtaaatcc gccgccagtt
    11341 gctcaagagt atgcttcgtc atgaccattc cttaagggcg tgcgaggaaa ttcagcacca
    11401 attgattgaa agcgtcggca tgttcccact gcgcccagtg accacagtcg cggaagatat
    11461 gcagttcaga accggcaatg ccggacagca gacgcagacc cgcatccatc ggcacaaagc
    11521 ggtcgttgcg cccccagaca atcagggttt gcgctttgat ttccgccaga cgtgggccaa
    11581 aatccgggaa ctgtttcgga ttagcttcca ggctcttaac gaagttttcc aggtgatcgc
    11641 ggcgcgacag catattattc aggcgcgctt caaacagggc gtcggtcaaa tcgctggtat
    11701 cgaaaacgaa gatatccatc atcagcttca ggttttcgat agtcggctga cgataaagct
    11761 gattcagtcg cttaatacct tcggttggca tcggcgtaaa caaactcatg ccgcccgtac
    11821 cgccgcccat cagcaccagt ttgccgacgc gctccggcca gttaagggtg aacgcca
//
LOCUS       DAJVRF010000121        11691 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJVRF010000121 DAJVRF010000000
VERSION     DAJVRF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11691
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.121"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..611)
                     /locus_tag="OQN13_003081"
     CDS             complement(<1..611)
                     /locus_tag="OQN13_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCS1616368.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
     gene            complement(734..2380)
                     /gene="fumB"
                     /locus_tag="OQN13_003082"
     CDS             complement(734..2380)
                     /gene="fumB"
                     /locus_tag="OQN13_003082"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS1616369.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="OQN13_003083"
     CDS             complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="OQN13_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCS1616370.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="OQN13_003084"
     CDS             complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="OQN13_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCS1616371.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5085..6716)
                     /gene="dcuS"
                     /locus_tag="OQN13_003085"
     CDS             complement(5085..6716)
                     /gene="dcuS"
                     /locus_tag="OQN13_003085"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCS1616372.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6897..7127
                     /locus_tag="OQN13_003086"
     CDS             6897..7127
                     /locus_tag="OQN13_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCS1616373.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7139..7411
                     /locus_tag="OQN13_003087"
     CDS             7139..7411
                     /locus_tag="OQN13_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1616374.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7638..7934
                     /gene="ghoS"
                     /locus_tag="OQN13_003088"
     CDS             7638..7934
                     /gene="ghoS"
                     /locus_tag="OQN13_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCS1616375.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7962..8135
                     /gene="ghoT"
                     /locus_tag="OQN13_003089"
     CDS             7962..8135
                     /gene="ghoT"
                     /locus_tag="OQN13_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HCS1616376.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="OQN13_003090"
     CDS             complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="OQN13_003090"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCS1616377.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="OQN13_003091"
     CDS             complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="OQN13_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HCS1616378.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11524..>11691)
                     /locus_tag="OQN13_003092"
     CDS             complement(11524..>11691)
                     /locus_tag="OQN13_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616379.1"
                     /translation="PGEMITEESRPVLEFLQMLCEIGTHYPGFETDIHGAYRQADGRY
                     TVKVLKEESKK"
BASE COUNT         3021 a         2834 c         2871 g         2965 t
ORIGIN      
        1 aagcgccgta gctggcggca atgaaagcga gcgaggaata tcaccgtcgt taatgcgcca
       61 agcagcgcgc agaacatatc ggattgcgtg tcccactggt cgccctgggt gccgagaaaa
      121 tcatccgctc cctggcccat tgccagcgcc gcccaccact cgattaattc atacatcgcg
      181 cttatcgcca gcgccacgca gcagactagg aacgccacca ttttacgtcc gcgcacgtac
      241 atcccgcgca cgagaatttc tcgtgccacc agtgcaggca ccagcccctg gaaaaaatgc
      301 cccagcttgt catacggatt acggctcaac cccagccatt cctgcacctc aaaaccgatg
      361 gggactttcg cgtaggtgta ttgcccgccg accatcagga tgatggcgtg aaagaaaatg
      421 agcgtataga gcagcggcgt taacggataa cgtttagcgg tggcaagcag tagcggcacg
      481 acaataatca ccggtgtcac ttccatcagc caggtgagtt tgtcatgggc cgaaatgccg
      541 gtataaatca ggattaacgt tagcgccagt gcgccggtgt taagaattaa cggtttgagt
      601 gtgcgggtca tggtgagttc gcaagtcagg gaaaatactg actattcacc agcaagcatg
      661 aaattacaat cgcaaaagag gtggcccagg gggatcacct ggcagcatgc tgccaggcgc
      721 tgggccgaag aggttactta gtgcagttcg cgcactgttt gttgacgatt tgctggaaga
      781 agtcgttacc tttgtcatcg accaggataa acgccgggaa atcttctact tcgattttcc
      841 agatagcttc catacccagc tccggataag cgacgcattc cagatgcttg atgctctgct
      901 gcgccagtac cgccgccgga ccgccgatgc taccgaggta gaagccgccg tgtttatgac
      961 acgcgtcggt aacctgctgg ctgcggttgc ctttcgccag catgatcatg ctgccgccgt
     1021 gggattgcag cagatccaca taggagtcca tacgacctgc ggtggttggg ccaagtgaac
     1081 ctgatggata accggcagga gttttcgccg gacccgcgta gtagatcggg tgatctttga
     1141 tgtactgcgg aagttcttta ccggcgtcaa tcagctcttt cagcttggcg tgtgcaatat
     1201 ctcgacccac aataatggtg ccagtgagcg acaaacgagt ggaaaccgga tattgcgaaa
     1261 gctgggcgag gatctctttc atcgggcggt taaggtcgac cttcaccgct tcgccttcac
     1321 cggcctgacg cagttcttgt ggaatgtact gacctggatt gtgttccagt ttttcgatcc
     1381 agataccttc gcggttgatt ttcgctttaa tgttacggtc agcggagcag gagacgccca
     1441 tgcctaccgg gcaggatgcg ccgtgacgtg gcagacggat aacgcgaatg tcgtgcgcga
     1501 agtatttacc gccaaactgc gcgccaagac cgagtttctg ggcctcttcc agcagttcct
     1561 gttccagctg gacatcgcgg aacgcctgac catgttcgtt cccttccgtc ggcagttcat
     1621 cgtaatagtg agcgcttgct aatttgacgg ttttcaggtt ggtttccgca gacgtaccgc
     1681 caatcacaaa cgcgatatgg tacggcgggc aggctgcagt accgagggta cgcattttct
     1741 cgacgaggaa gtttttcagt ttgccgggag tcagcagggc tttggtttcc tggtagagat
     1801 acgttttgtt ggcagagccg ccgcctttcg caacgcaaag gaatttgtac tcatcgccat
     1861 ctaccgcgta caggtcgatt tgcgcaggca ggttagtgcc ggtgttgacc tctttgtaca
     1921 tgtccagcgc cgcgttttgc gagtagcgca ggttatcttc gatataggtg ttatagacgc
     1981 ctttcgacag cgcttcttca tcaccgccac cggtccacac gcgctggcct tttttaccga
     2041 cgatgatcgc ggtgccggta tcctggcagg tcggcagcac gcctttggcg gcgatttcgg
     2101 agtttcttaa gaattgcagt gccacgtact tgtcgttttc gctggcttct ggatcgtgaa
     2161 gaatagccgc aacctgtttc tggtgtgccg gacggagcat gaaagaagcg tcgtgaaagg
     2221 cttgctgcgc cagcagggtc agggcttctg gttccacttt caggatggtt tcgccgtcga
     2281 agtcggcaac gctaacgtaa tcggaagtga gtagatagta ttcggtattg tctttcccca
     2341 tcgggaaagg tgcctggtag ataaagggtt tgtttgacat agcttccagc ctgtaactct
     2401 gtatttgtta ttcgaaaatg gcgtgccgcg tagtcacggg cggcacgcaa agtgcattta
     2461 taagaacccg tacatcgcgg cgaagatcca gccgaagacg cacgatacgc tcacaccaat
     2521 caaccccggc agaataaagc tgtggttgat gacgaagcga ccgatgtggg tggtgccgga
     2581 acggtcaaac tgaatcgctg ccagatcgct cggataagtc ggcaggatgt aataaccgta
     2641 gcaagccggt gctgaagcca cgatgtatgc cggatcaacg ccgatcgcca gcgcgaccgg
     2701 aacaatcgcc gccagcgccg cagcctgaga gtttacaaac ttggaaacca gcagcagaac
     2761 aatggcatag gcccacggat actctttcac catttcaccc agtacgccct gaatttcaga
     2821 catatgcgca ccgaacatgg tttctgccat ccatgcgata ccgtacaccg ccacgatggc
     2881 gatcataccg gaacggaaga cttcgttttt tgagatagac gcgggattgg ttttggtcag
     2941 gataataatc agcgccccgg tcagcagcat aaacatctga ataaccagta ccatcgacag
     3001 cggtttgccg ccgaaggatg gacgcaggtc cgaatcagca ccaagaaggg cgactacagc
     3061 gattgccccg aggaaaatcc acattgccag ccagttgctt ttcggcagtt ttttgtccag
     3121 cagcgtcgcg gtatcaccgt aaacatactc acggttttcc ggtacggaga tgaatttctg
     3181 gaactcttcg tctttatcca gatctttacc acggaaccag ctgaagatac cgatcgccag
     3241 gataccgatt aacgtcgatg gaatggtgat tgccagcagg tcgaggaact caagatggcg
     3301 accatcaaag gtgacattac ccagcattgc aaccagagac acgaccgcaa ccgacaccgg
     3361 actggcgatg atccccatct gtgcaccgat agaacttgcc gccatcggac gttccggacg
     3421 gatgttgttc ttaatggcga cgtcgtagat gatcggcaga atggtgtaaa ccacatgacc
     3481 cgtaccgcaa agaatggtca gcgtacaggt cacaaacggc gcgacaattg agacatattt
     3541 cgggttgcgg cgcagcagct tctcggcaat ttgcagcatg acatcaagac cgcccgaagc
     3601 ttgcaaggtc gccgatgccg ccaccaccgc aatgataacc agcatgacat caaccggtgg
     3661 tttacctggc tgaaggtgga agacgaagac cagaatgacc agaccgatac cgcctaataa
     3721 acccagcgcg ataccaccct ttctggcacc ataaaacaga catatcagta ttatgataag
     3781 ttggatagta aataacatgt gtgaaccctc gcgataatcc tatttaaatt tttgctgaat
     3841 agatcacagt cacgttctgt tttgtatgaa ctgttttcag agcctgacta aatatccgtt
     3901 tggtcgttac cggctttagc aaatacctca cagtgaatat tggctgatta aagcagcggc
     3961 aaatttatgc actgtgataa gcggcttttt cagagagaaa gactctctgg cgatgggttt
     4021 atagtttgta ctgcgcagat gttaatgttt tgtttgctgt tatcttattg atatttatcg
     4081 gtctattttt tgttctcagg gcagatgtca ttaggtttat agattaatct gatctaccca
     4141 tttgtgggta aaaatacaca taatgcgggt gacataatag ttaattaact tttgttagcg
     4201 ttttgaaatt aaaaacaccg ttcacctgaa gagatattaa tttttagcga tgatgcaggg
     4261 ataattatat ttgatctggc acaagtttta ctgatgaagg atgtaacttg tgccaggggt
     4321 attttgcatt acggtaataa ttattactta cagataacca gcgatccgtt attggcaata
     4381 ttgtttcagt agtgagtagt gttctgcctg aatacggtaa cggtaaactg gacgccccgt
     4441 gacgccataa tggatactgg tgaacaagat gtggcagttg accagccaga tgaggtattt
     4501 acggcaggaa acacgcgaaa tgttaacctc gttggctagc tcgtcggttg aaaattcata
     4561 gtcctgatgc gcgtcaatcc actggcacag tgtgcgtaac gtctgcggcg ttaagccttt
     4621 tggcaagcga cgaggatcct gttcgttgga gctgctgccg tggattagct gatcaagctc
     4681 ggcctggtca taatactgat gtttttccag cgccattttc ttttgccgcc agtcggtgag
     4741 cgcctcttca aagcgggaag cctggaaggg tttgatcagg taatccacga caccgtaatg
     4801 cagcgaatct ttaatggttg ccgcatcggc tgcggaggag atgacaatca catcactttt
     4861 gcaacgcgcg ttatgcagga caggcagtaa atcgagcccg ttctcttttt gcatatagat
     4921 atcgagcaat atcaggtcga taggcgtatc gctattgaag ataatctctt tggctttctc
     4981 cagcgtcgag gctgttccac agcattgaaa gcctgggatt tgtgctacgt atcggcgatt
     5041 cagctccgcg accattgcgt cgtcatcgat aattaataca ttgatcatct gttcgacctc
     5101 tccccgtccc agggtatctg gacaaaaaat tgtgtgaaaa tcccgggttc cgattccacg
     5161 gcgatgctgc cgccgagatt ttctacctgt tgtttgacaa gtgctaaacc gacgcctcgc
     5221 tcgcttcctt ttgtcgagac acctttgtca aaaatgtgat cgattttgtc gggtgcgatc
     5281 cccggtccat catcgttaac ttcgcagtgc agccagccgt gacggtagtg caatgttacg
     5341 ctaatttcgc caccgggttc cggccctaat gcttccagcg cgttttctat cagatttccc
     5401 aatgtggtaa tcagcgtcgc gacctggtcc tcactgccgc tgtctggcag ctggctttca
     5461 ctgtttaaaa tcagcgtatg gcctaaatcg gtcgcgcgat taatcttgct gattaaaaaa
     5521 ccagcgataa ccggagattt gatcttaccc agcagagagc caatctcttc ctgatagtta
     5581 ttggctgttt ttagaatgta atcttccaac tgcttataac tcttcagatg caataatccg
     5641 agaatcacgt gcaatttatt cataaattcg tgggatcgtt cacgaagtgc gtcggcatag
     5701 ttgaccagac cgtcgagtcg ctgcatcagt ttacgtactt cggttttgtc cctgaaggtt
     5761 gaaatggcac cgatgataac gccattactg cgcaccggaa cggtgttgat cagtaatagc
     5821 cggtctttaa tcgtaatctc ttcgtcgcgg cgcggcgtac cgtcgcgtaa cacttccgag
     5881 acatctacca cctgtgacca tgagtggctg agcgtcgaca gtttctcatc gtcctgcgac
     5941 ttacggtaat tcagcaattc ttgtgcggca tcgttgatca gcgtgacctc gccgcgatcg
     6001 tccacggcaa cgacgccttc tttgatagac tgcaacatgg cctggcgttg ctcaaacagc
     6061 gtggagattt cgtagggttc caggccgaaa aggatttttt tcagtacctt aaccagaatg
     6121 caggtgccaa tcagtccgac cagcatgcca aataataccg accagataat gctccagcga
     6181 ctgtcattga tttgttgggt cacgcggctt aactcaaggc cgatcgccac cacgccaatt
     6241 tgtttatgat tttcatcgta gatgggggta aatacgcgta aagcctgcgc cagaaaaccg
     6301 cgattgatag cgacattttc tttgccattc agcgctttaa ggatgtcatc acctttaaat
     6361 ggctgaccaa tacgctgggc ttcaggatgc gagtagcgaa gactttgcat atcggtaacg
     6421 acaataaaca gcagatcgtt gcgtttgcgc acggcttccg cgatggcctg gatgccgctc
     6481 tcctgcggtt ttttctgcaa gccctgacgg atttccggcg agttggcgag ggtacgcgcc
     6541 actgccagtg ccttgttggc tagcccgtct cgcgtcatat cactgatttg cgagaagtaa
     6601 atcagatgca ccaccaatag caccgagaac agtaccgcac tgaccattaa gatcactgtg
     6661 gtactcaatt tcatcggacg tttgcgtaac atgcggtagg gcaatgaatg tctcatcagc
     6721 ttccttgtgt gacaaatttc ttaaacatta tctctgatga ggtgggtaat tcaaagggag
     6781 taagaatgat tggctatata ggggaagaga ttctggcaac ggaaactgcc agtgctgtat
     6841 gaagattcca gggctatgct tatagcgata atcatactga tgagagaggg aaagtcatgg
     6901 atcaggcgct actggacgag ggttatcgct gttataccgg cgaaaagatc gatgtctatt
     6961 tcaacactgc gatatgtcag cattctggca attgcgtacg tggcaacggc aagttattta
     7021 atctcaaacg aaagccgtgg atcatgccgg atgaagtcga cgtcgccact gtggttaaag
     7081 tgattgatac gtgcccgagc ggcgcgctga aataccgtca taaataagcg agggtaaaat
     7141 ggaaatacgc gaaggccaca ataaatttta cattaatgac gaacaaggca agcaaatcgc
     7201 tgaaattgtc tttgtgccga ctggagagaa tttagcgatt atcgaacata ccgatgtcga
     7261 tgaaagcctg aaagggcagg ggattggtaa acagctggtt gcaaaagtcg tggaaaaaat
     7321 gcgtcgggaa aaacgaaaaa ttatcccact atgcccattt gcgaaacacg aatttgataa
     7381 aacgcgggag tatgatgata ttcgcagttg atgggagagt acagagtcac gatatttttc
     7441 atgctctccg cggagtgatg caggagagca tctgaagggt agggggatgc acaaagaatg
     7501 ggcagagagc gcgttttttt gtcccaagtc atccccttta ctgagcaaaa aaaagaatat
     7561 ctcctatatg agaatcatca atcggggtta tcaagttttg cgtccccaga gcattcaata
     7621 ttgataggag tcatattatg gaaggtaaaa acaagttcaa tacttatgtt gtttcttttg
     7681 attatccatc atcttattcc tcagtgttct taaggttaag atcattgatg tatgatatga
     7741 atttctcttc tatcgtggct gatgaatatg ggataccacg acaattgaat gaaaattcct
     7801 tcgcaataac gacatcgtta gccgcaagtg aaatcgaaga tttaatcagg cttaaatgct
     7861 tagacttacc ggatattgat tttgacctca acattatgac agttgatgac tatttccgtc
     7921 agttttacaa gtagccagga aggataagaa gaacataaaa aatggcacta ttctctaaaa
     7981 tattaatttt ttatgtgatt ggtgtgaaca tatcctttgt cattatctgg tttatctcac
     8041 atgagaaaac acatattcgt ttacttagtg cattcctggt cggaataacc tggccaatga
     8101 gtctgcctgt ggcattactt ttttctctct tttaggagct gtagttgagt ggacccgctg
     8161 gttatcgtga aacgaagcgt ctctcctcaa aactggattt gcggaacgac gaacgaagca
     8221 gtcgcctgac tgcttcgttc attaaagtga aatttatttc tgtgggcgca tcgccgggaa
     8281 gagaataacg tcgcgaatag tatgactgtt tgtaaacagc ataatcattc ggtcgatacc
     8341 aatacccaga ccagcggttg gcggcagacc atattccagc gcagttacgt aatcttcgtc
     8401 atagaacatg gcttcgtcgt cacctgcagc tttagcgtta acctgttcct ggaaacgttc
     8461 agcctgatct tctgcgtcgt ttaattcgct aaaaccatta ccgatttcac gaccaccgat
     8521 gaagaattca aaacggtcgg tgatttccgg gttaacatca ttacggcgcg ccagcggtga
     8581 cacttctgcc ggatattccg taataaaggt tggctgaatc agatgtgctt ctgccacttc
     8641 atcaaagatc tccgtgataa tacgtcccag cccccagctt ttctctaccg taattccgat
     8701 agattcagct aatgctttag cagcatcaaa attatccagg tcggccatat cggtttctgg
     8761 acgatatttt ttgattgctt cgcgcatggt gagtttttca aacggtttgc cgaaatcaaa
     8821 cacatgctcg ccataagtga ctttagtggt acccagaacc tcttgtgcca gggtgcggaa
     8881 cagtgactct gtcagttcaa tcaaatcgtg gtaatccgca tacgccatgt agagttccat
     8941 cattgtgaac tcaggattat ggcgaacaga aataccttca ttacggaagt tacggttgat
     9001 ttcgaatacc cgttcaaaac cgcctacaac cagacgtttc agatacagct ccggcgcgat
     9061 acgcagatac atatctaaat ccagagcatt atgatgggta ataaacgggc gagcagatgc
     9121 cccacctgga attacctgca tcatcggggt ttctacttcc ataaagccgc gcgcgaccat
     9181 gaactgacgg atagcggcca gaatttttga acggacaaca aacgtttgac gggatttatc
     9241 gttagcgatg aggtccagat aacgttgacg ataacggact tcctgatcct gcagaccatg
     9301 gaatttatct ggtaaaggac gtagtgcttt agtcagcagg cgcaactcag tacagtgaat
     9361 ggaaagctca cccgtttgcg tcttaaacag cgtaccgcgg gcaccgataa tgtcacccag
     9421 atcccatttt ttaaactgat cgttataaac accttctggc aggctatctc ttgcaacgta
     9481 cagttgaata cggccaccga catcctgcaa cgttacaaag gaggctttcc ccatgatacg
     9541 acgggtcatc attcggccag caaccgagac ttcaatgttt aaggattcca gttcctggtt
     9601 attcttcgca tcaaactctt cgtgcaactg gtcagaggta tggtcgcggc gaaaatcatt
     9661 gggaaacgcc acaccttgct gacgtagtgc cgccagtttt tcgcggcgat ttctcagttc
     9721 atcgttaaaa tcaatagcct cattggctcc ccgtgtttct tgttcagaca ttttggttcc
     9781 tctaaatcca gctttcaatt tagcgtagat aaagagacag atcggtacac tgtattatct
     9841 gcctcgacta ttaataactc agtttatctt atcgaaacta acgcttacct caagttgata
     9901 tcactaataa ataaccatca aaatggttta aatgacttat taatctcgat ttactagtgt
     9961 gattaactga aagttaattt attcataacc aattgttttt acgacagtta atcgttgctc
    10021 tcctgtatca tattcgtcgg cgtgctaaac agaaaacggg tagcaaaggc caatacgacg
    10081 atgatcgcga cacaagccaa cgtccactct cccatctgag aaaagaatcg ctggtaagct
    10141 gcaattacca taccgctaat ttgcgactct gtcgtctgct gtgccacgac gcctgccagc
    10201 cagttggcga ccgcgcctgt tgccagcata taaataccgg ttaatacgcc agacattttc
    10261 agacgcgtga tttgcgcaat cgccaccggg tcaataaaga gttctgcaaa gcccattagc
    10321 gccaacccgg atatcatcac gcccattgac gcttgaccgt cagccgctgc atgtcgggca
    10381 tcaaatgcca acaacataaa gccacaagcc atcagcagta agccaaaggc aaacttcagc
    10441 cagacgcgca atgttgagtt gccgcggctt tctggcgacg ccagccaggc cagtacaacc
    10501 ccagcgagca tcactgcaat tgcattcacc gactggaata gtgctgtagg tacttcaata
    10561 ttgaatgcct gacgattcac aaagcgatcg ataaacaagc tgatggtact gccgccctgt
    10621 tgtgccagta cccagaacaa cgtcccgaca aacatcaaca atacaatttg ccaaagagca
    10681 cggcggtgtt cggggaattt gatcatcatg cgggcaatga tttgtgcggc aatgaggcaa
    10741 acgatcgcca gcaaatatcc ggaccagtcg ttctccagca gcagggtaaa aaatactggg
    10801 gctaaacaga gcatcaccac taaccagctc catactggta aggcaaattt gacgctagtg
    10861 agcgcttttt tatccatact acgcgtggac tggaaatgac gatgaccgct taagaaaatc
    10921 aacaaaccga taaacatgcc gccacccgca agggcaaagc caacatgcca tccataccac
    10981 tgagcagcca ggccgcaggc gattggggcg gcgatagaac cgatattgcc cgcagcatac
    11041 agcagcgaaa aaccgccatc acgtcgatga tcgttctcgt cgtagagctc gccaagcaaa
    11101 cagctgatgt ttgatttgaa taaaccgtag ccacaaataa tgattgccag cgccagatac
    11161 aggctaaagg ttgaatttgt atcaatgccc agcaccacat ggccaagggt cattaacagc
    11221 gcgccggcaa tcactgcagt gcggttgccg agcaggcggt cggcaagcca gccgccgaga
    11281 ataggggtaa cgtaaaccag agaagcatat gcgctgaaca ggctgatggc atggttatca
    11341 tcaaaaccaa gctgatgggt gagatagaga atgagtaagg cacgcatgcc gtaaaaactg
    11401 aagtactccc agatttggat cgccacgata tagtatatcg cgcgcggctg tgagggtgtt
    11461 ttcatgtgtt ctccttatga gcaaaaaagg gaagtggcaa gccacttccc ttgtacgagc
    11521 taattatttt ttgctttctt ctttcaatac cttaacggta tagcggccat cagcctgacg
    11581 gtatgcaccg tgaatatcgg tttcaaatcc cggatagtga gtgccgattt cacacagcat
    11641 ctgcaggaac tccagaaccg gacggctttc ttcggtgatc atttcacccg g
//
LOCUS       DAJVRF010000122        11690 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJVRF010000122 DAJVRF010000000
VERSION     DAJVRF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.122"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..1490
                     /locus_tag="OQN13_003093"
     CDS             <1..1490
                     /locus_tag="OQN13_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HCS1616380.1"
                     /translation="DGPETPEEEKGYWFHAYQPEDLCTIHNPMGDLQDFIALVKDAKK
                     FGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKISKDIEGGIPGYWQGQVLIPFIKD
                     PVTKERKQIHPEDIHLTAKDFEASKDNISKDEWENLHALKEKRLNGIPKTTPKSDQVI
                     MLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSITPPLKNYNERLHNTNLFNPKYHK
                     KAVMNYMEYLVTCQLDEQQMSSLLYERDDLSAIDFSLLMKTIKAFSFGGDLLTLASKP
                     GSTISSIPSERRILININHDFPNNGNLFNDFLFNHQQDEQLAMAYMAALPFSRPLVYW
                     DGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQLYNEFHALYIDKAGIWSAFEGVF
                     ATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEGTWPNKLQPGIYKNMGSNSVNII
                     INNTRKIIPPGKVFTLRGGTLNINIPGRSALLLGKTGEPPNYLYL"
     gene            1826..2803
                     /gene="csiD"
                     /locus_tag="OQN13_003094"
     CDS             1826..2803
                     /gene="csiD"
                     /locus_tag="OQN13_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCS1616381.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            2823..4091
                     /gene="lhgO"
                     /locus_tag="OQN13_003095"
     CDS             2823..4091
                     /gene="lhgO"
                     /locus_tag="OQN13_003095"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCS1616382.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSKHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4114..5562
                     /gene="gabD"
                     /locus_tag="OQN13_003096"
     CDS             4114..5562
                     /gene="gabD"
                     /locus_tag="OQN13_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HCS1616383.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5576..6856
                     /gene="gabT"
                     /locus_tag="OQN13_003097"
     CDS             5576..6856
                     /gene="gabT"
                     /locus_tag="OQN13_003097"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCS1616384.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7167..8567
                     /gene="gabP"
                     /locus_tag="OQN13_003098"
     CDS             7167..8567
                     /gene="gabP"
                     /locus_tag="OQN13_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCS1616385.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            8588..9250
                     /gene="csiR"
                     /locus_tag="OQN13_003099"
     CDS             8588..9250
                     /gene="csiR"
                     /locus_tag="OQN13_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCS1616386.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9251..9700)
                     /gene="kbp"
                     /locus_tag="OQN13_003100"
     CDS             complement(9251..9700)
                     /gene="kbp"
                     /locus_tag="OQN13_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCS1616387.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(9784..9942)
                     /locus_tag="OQN13_003101"
     CDS             complement(9784..9942)
                     /locus_tag="OQN13_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCS1616388.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10125..10424
                     /locus_tag="OQN13_003102"
     CDS             10125..10424
                     /locus_tag="OQN13_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1616389.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10434..10958
                     /gene="ygaP"
                     /locus_tag="OQN13_003103"
     CDS             10434..10958
                     /gene="ygaP"
                     /locus_tag="OQN13_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCS1616390.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(11005..11409)
                     /gene="stpA"
                     /locus_tag="OQN13_003104"
     CDS             complement(11005..11409)
                     /gene="stpA"
                     /locus_tag="OQN13_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCS1616391.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2895 a         2881 c         3035 g         2879 t
ORIGIN      
        1 ccgacggacc agaaactccc gaagaagaaa agggttactg gtttcatgct tatcaacccg
       61 aagatctttg taccatccat aatccaatgg gagatttgca ggattttatt gcattggtta
      121 aagatgctaa aaaatttggt atcgatatca ttcctgatta tacctttaac tttatgggaa
      181 ttgggggtag tggtaaaaat gacctggatt atccctctgc tgatatacga gcgaagatca
      241 gtaaagatat agaaggtggt atccctggct attggcaagg tcaggttttg attccattca
      301 ttaaagatcc agtaacaaaa gaacgtaaac aaatccatcc agaagatata catctcactg
      361 caaaagactt cgaagcaagt aaagataaca tctctaagga tgaatgggaa aacctccatg
      421 cattaaaaga aaagcgttta aatggaattc ctaaaacaac acccaaaagt gaccaggtta
      481 ttatgttgca aaatcaatac gttcgtgaaa tgcgaaaata tggcgtacga ggtttacgtt
      541 atgatgcggc aaaacactca aaacatgaac aaatagaaag atcaataacc ccaccgctta
      601 aaaattataa tgagcggtta cacaatacta acttatttaa cccaaaatat cataaaaaag
      661 ccgttatgaa ttacatggaa tatctggtaa cttgtcagtt ggatgaacaa caaatgtcat
      721 cactgcttta tgaaagagat gatttaagcg ccattgattt ttcattgctc atgaagacga
      781 taaaagcctt ttcattcggg ggagatctcc taacccttgc atcaaaaccg ggttccacaa
      841 tctcaagcat cccatcagaa agacggatat tgattaacat taaccacgat tttcctaaca
      901 atggcaatct tttcaatgac tttctattta accatcaaca agatgaacaa ttagcaatgg
      961 catatatggc cgctctcccg ttcagcagac ctttagttta ctgggatggc caggtattaa
     1021 aatcaacgac tgaaattaaa aattatgatg ggtccacgcg tgtcggtggt gaggcgtggc
     1081 ttaataaagg ttgctctacc tatcagcagc tctacaatga attccacgca ttatatatag
     1141 ataaagcagg aatatggagc gcatttgagg gtgtatttgc aactaaaaac gttctagcct
     1201 ttagtcgtgg ggattctgtg aacattaatc actctcctca tgatggacta gttataataa
     1261 ataaaggaaa cgaagaagtt gaaggtacct ggcctaacaa attgcaacct ggaatatata
     1321 aaaacatggg gagtaatagc gttaacatta ttattaataa tacccgaaaa attatccccc
     1381 ctggtaaagt atttacgctt agaggcggaa ctctaaatat caatattcct gggcgtagcg
     1441 ctcttctttt agggaaaaca ggagaaccgc cgaactatct ctatttataa tttaatgtta
     1501 tatctgcccc gataaaacgg ggcagataat atgtttagtt tactaacggt cattttgcag
     1561 tgaagccatt tactgttttt tatcgaccag ataatctgtt ctctaatgtt aactccccct
     1621 aacctgttgc tttagttatt catttcctgt ctcactttgc cttaatactc tacgttaaat
     1681 gttactaatt tgttgctttt gatcacaata agaaaacaat atgtcgcttt tgtgcgcatt
     1741 tttcagaaat gtagatattt ttagattatg gctacgaaat gagcatcgcc atgtcaccct
     1801 acatctcata agaggatcgc ttctgatgaa tgcactgacc gccgtacaaa ataacgctgt
     1861 cgattcaggc caggactata gcggattcac tctcatcccg tcggcgcaat ccccgcgtct
     1921 gctggaactc accttcaccg aacagacgac caaacagttt ctcgagcagg ttgccgaatg
     1981 gcccgtacag gcgctggagt acaaatcgtt tctgcgtttt cgggtaggca aaattcttga
     2041 cgatctgtgt gcgaatcagc tgcaaccatt gctgttgaag accctgctaa accgtgctga
     2101 aggtgcgctg ttgatcaatg cggtgggtat cgatgatgtg gcgcaggcag atgagatggt
     2161 gaagctggca acggcggtag cgcatctgat tggtcgctca aatttcgatg cgatgagcgg
     2221 tcagtattac gcgcgtttcg tggtgaaaaa tgtcgataac tcagacagct atctgcgtca
     2281 gccgcaccgc gtgatggaac tgcacaacga cggcacgtat gtcgaagaga tcaccgatta
     2341 cgtgctgatg atgaaaatcg atgagcaaaa catgcagggt ggaaattcgt tgctgctgca
     2401 tctcgatgac tgggaacatc tggaccacta tttccgccat ccactggcgc gtcgcccgat
     2461 gcgctttgcc gcgccgccga gcaaaaacgt cagcaaagat gttttccatc cagtgttcga
     2521 cgttgatcaa cagggtcgcc cggtgatgcg ctatatcgac cagttcgtcc agccaaaaga
     2581 ttttgaagaa ggcgtgtggt tgagcgagct ttccgatgcc attgaaacca gcaaaggcat
     2641 tctttctgta cccgttcccg ttggcaaatt cctgttgata aacaacctgt tctggctgca
     2701 cggtcgcgac cgctttactc cgcacccgga tctgcgccgt gaactgatgc gtcagcgtgg
     2761 ctatttcgct tacgccactc accactacca gacgcatcag taagcgcaaa ggaattgagc
     2821 ggatgtatga ttttgtgatt attggcggcg gcatcatcgg catgtcgacc gccatgcaac
     2881 tgattgatgt ttatccggat gcccgcattg cgttgctgga aaaagagtcc ggcccggcct
     2941 gtcaccagac gggccacaac agcggcgtga tccatgccgg ggtctattac acgcccggca
     3001 gcctgaaggc gcagttttgc ctggcgggga accgcgccac caaagccttt tgcgatcaaa
     3061 acggcattcg ctatgacaac tgcggcaaga tgctggtcgc cacgtctcag ctcgaaatgg
     3121 aacggatgcg cgccttatgg gaacgcacag cggcgaacgg tatcgagcgc gagtggataa
     3181 acgccgtgga actgcgcgag cgcgaaccga atatcaccgg gcttggtggc atttttgtgc
     3241 cgtccagcgg cattgtcagc taccgcgaag tcacagcggc aatggcaaaa atcttccagg
     3301 ccagaggcgg cgagattatt tataacgccg aagtcagcgc cctcagtaag cataaaaacg
     3361 gcgtggtgat acgcacccgt cagggcggtg aatatgaagc atcgacgctg attagctgtt
     3421 ccgggctgat ggctgaccgg ctggtgaaaa tgctcggcct cgaaccgggc tttatcatct
     3481 gcccgttccg tggtgagtat ttccgccttg cgccggagca taaccagatt gttaaccacc
     3541 tgatttaccc cattcccgac cccgcgatgc catttttggg cgtgcatctc acccgcatga
     3601 tcgacggcag cgtgaccgtc gggccaaacg cggtgctggc tttcaaacgc gaaggctatc
     3661 gcaagcgcga cttctcattt agtgacacgc tggagatttt gagctcgtcg gggattcgcc
     3721 gggtgctgca aaaccatcta cgctcaggac tgggcgagat gaaaaactcg ctgtgcaaaa
     3781 gcggctacct gcggctggtg caaaagtatt gtccccggct ttcgttaagc gatctccagc
     3841 cctggcccgc cggtgtgcgg gcgcaggcag tatcgccgga cggcaagctg attgacgatt
     3901 ttctgtttgt caccacccca cgcacgatcc acacctgcaa tgcgccctcc ccggcagcga
     3961 catcagcaat tcctattggt gcgcatattg tcagcaaggt acaaacgctg ttggcaagcc
     4021 agagtaaccc cggacgcacg ctgcgagcgg cacgtagtgt ggatgcctta cacgccgcat
     4081 ttaatcaata acctttgaaa acaggacgta acaatgaaac ttaacgacag taacttattc
     4141 cgccagcagg cgttgattaa cggggaatgg ctggacgcca acaatggcgg gatcatcgac
     4201 gtcaccaatc cggcgaacgg cgacaagctg ggtagcgtgc cgaaaatggg cgctgatgaa
     4261 acccgcgccg ctatcgacgc cgccaaccgc gccctgcccg cctggcgtgc gctcaccgcc
     4321 aaagaacgcg ccaacattct gcgcaactgg ttcaatttga tgatggagca tcaggacgat
     4381 ttagcgcgcc tgatgaccct cgaacagggc aaaccactgg ccgaagcgaa aggcgaaatc
     4441 agctacgctg cctcttttat tgagtggttt gccgaagaag gcaaacgtat ttatggcgat
     4501 accattcccg gtcatcaggc cgataagcgc ctgattgtta tcaagcagcc gattggcgtt
     4561 accgcagcta tcacgccgtg gaacttcccg gcggcgatga ttacccgtaa agccggtccg
     4621 gcgctggcgg caggctgcac catggtgctg aagcccgcca gtcagacacc gttctctgcg
     4681 ctggcgctgg cggagctggc gattcgcgcg ggcattccgg ctggcgtgtt taacgtagtc
     4741 accggttcgg caggcgcggt cggtaatgaa ctgaccagca acccgctggt acgcaaactg
     4801 tcgtttaccg gttcgaccga aattggccgc cagttaatgg aacagtgcgc gaaagacatc
     4861 aaaaaagtgt cgctggagct gggcggcaac gcgccgttta tcgtctttga cgatgccgat
     4921 ctcgataaag ccgtggaagg cgcgctggcc tcgaaattcc gcaacgccgg gcaaacctgc
     4981 gtctgcgcca accgtttata cgtgcaggac ggcgtgtatg accgctttgc cgaaaaattg
     5041 cagcaggcgg tgagcaaact gcacatcggc gacgggctgg ataaaggcgt caccatcggg
     5101 ccgctgatcg atgaaaaagc ggtagcaaaa gtggaagagc atattgccga tgcgctggag
     5161 aaaggtgcgc gtgtggtttg cggcggtaaa gcggatgaac gcggcggcaa cttcttccag
     5221 ccgaccattc tggtggacgt gccggccaac gccaaagtgt cgaaagaaga gacgttcggc
     5281 cccctcgccc cgctgttccg ctttaaagat gaagccgatg tgattgcgca agccaatgac
     5341 accgagtttg gccttgccgc ctatttctac gcccgtgatt taagccgcgt cttccgcgtg
     5401 ggcgaagcgc tggagtacgg catcgtcggc atcaataccg gcattatttc caatgaagtg
     5461 gccccgttcg gcggcatcaa agcctcgggt ctgggtcgtg aaggttcgaa gtatggcatc
     5521 gaagattact tagaaatcaa atatatgtgc atcggtcttt aactggagaa tgcgaatgaa
     5581 cagcaataaa gagttaatgc agcgccgcag tcaggcgatt ccccgtggcg ttgggcaaat
     5641 tcacccgatt ttcgctgacc gcgcggaaaa ctgccgggtg tgggacgttg aaggccgtga
     5701 gtatcttgat ttcgcgggcg ggattgcggt gctcaatacc gggcacctgc atccgaaggt
     5761 ggtggccgca gtggaagcgc agttgaaaaa actgtcgcac acctgcttcc aggtgctggc
     5821 ttacgagccg tatctggagc tgtgcgagat tatgaatcag aaggtgccgg gcgatttcgc
     5881 caagaaaacg ctgctggtta cgaccggttc cgaagcggtg gaaaacgcgg taaaaatcgc
     5941 ccgcgccgcc accaaacgta gcggcaccat cgcttttagc ggcgcgtatc acgggcgcac
     6001 gcattacacg ctggcgctga ccggcaaggt gaatccgtac tctgcgggca tggggctgat
     6061 gccgggtcat gtttatcgcg cgctttatcc ttgcccgctg cacggcataa gcgaggatga
     6121 cgctatcgcc agcatccacc ggatcttcaa aaatgatgcc gcgccggaag atatcgccgc
     6181 catcgtgatt gagccggttc agggcgaagg cggtttctac gccgcgtcgc cagcctttat
     6241 gcagcgttta cgcgctctgt gtgacgagca cgggatcatg ctgattgccg atgaagtgca
     6301 gagcggcgcg gggcgtaccg gcacgctgtt tgcgatggag cagatgggcg tggcgccgga
     6361 tcttaccacc tttgcgaaat cgatcgcagg cggtttcccg ctggcgggcg tcaccgggcg
     6421 cgcggaagtg atggacgctg ttgcaccggg cggtctgggc ggcacctatg ccggtaaccc
     6481 gattgcctgc gtggctgcgc tggaagtgtt gaaggtgttc gagcatgaaa atctgctgca
     6541 aaaagccaac gatctggggc agaagctaaa agacggattg ctggcgatag ccgaaaaaca
     6601 cccggagatc ggcgacgtac gcgggctggg agcgatgatt gccatcgagc tgtttgaaga
     6661 tggcgatcac agcaaaccgg acgcaaaact caccgccgag atcgtggcac gcgcccgcga
     6721 taagggcctg attcttctct cctgcggccc gtattacaac gtgctgcgca tccttgtacc
     6781 gctcaccatt gaagacgctc agatccgtca gggtctggag atcatcagcc agtgttttgc
     6841 tgaggcgaag cagtagctat gtccgtttgt aagagttaac cgttgctaag ggaatgataa
     6901 cgatgcagta aaaaataata acgatgaggc gaacgacaaa gttaaaaacg actgcggaca
     6961 gtcccctcgc ccctctggag agagggttag ggtgaggggt aaaacacgcg gctaaaccct
     7021 cccctggccc tctccctaag ggagagggag aaaaccctcc cgctttcgtt tttaagattt
     7081 tgttgaccgt ctcaattaaa cgcgtgaccc ggcgcgggaa atgtcggggc gctctcccaa
     7141 gtgacacact ttcgagagga ttcaggatgg ggcaatcatc gcaaccacat gagttaggcg
     7201 gcgggctgaa atcacgccac gtcaccatgt tgtctattgc cggtgttatc ggcgcaagtc
     7261 tgtttgtcgg ttccagcgtc gccatcgccg aagcgggccc ggcggtatta ctggcctatc
     7321 tgttcgccgg gctactggtg gttatgatta tgcggatgtt ggcggaaatg gcggttgcca
     7381 cgcccgatac cggttcgttt tccacctatg ccgataaagc cattggtcgc tgggcgggct
     7441 ataccatcgg ctggttgtac tggtggtttt gggtactggt tatcccgctg gaagccaata
     7501 tcgccgctat gatcctgcac tcgtgggttc caggcattcc catctggtta ttttccctcg
     7561 tcattaccct tgccttaact ggcagtaatt tattaagcgt taaaaactac ggcgaatttg
     7621 agttctggct ggcgctgtgc aaagtcatcg ctatcctggc ctttattttc cttggtgcag
     7681 tcgcaattag cggtttttac ccttatgccg aagtgagcgg gatctcaaga ttgtgggata
     7741 gcggcggctt tatgcccaac ggtttcggtg cggtattaag cgcgatgttg atcaccatgt
     7801 tctcgtttat gggcgcagaa attgtcacca ttgccgccgc ggaatccgac acgccggaaa
     7861 aacatattgt ccgcgccacc aactcggtta tctggcgtat ttctatcttc tatttgtgct
     7921 ctatttttgt cgtcgtggca ttaataccgt ggaatatgcc ggggctgaaa gccgttggat
     7981 cttatcgctc ggttctggaa ttgctcaata ttccccatgc gaaattaatc atggactgtg
     8041 tgatattact ttccgtaacc agttgcctga actcggccct gtataccgcc tcaaggatgc
     8101 tctactcctt aagccgtcgc ggtgatgctc ccgcggtaat gggtaaaatc aaccgcagta
     8161 aaaccccgta cgtggcggtg ttactctcca ccggcgcggc attcttaacg gtggtggtga
     8221 actattacgc gcctgcgaag gtatttaaat ttctgatcga cagctccggc gctatcgccc
     8281 tgctggttta tttagtcatc gccgtttcac agttgcggat gcgcaaaatt ctgcgagcgg
     8341 aaggaagcga aattcgcttg cgaatgtggc tttatccctg gctcacctgg ctggtcatcg
     8401 gctttattac ctttgtgttg gtagtgatgc tattccgtcc ggcgcaacag ttagaagtga
     8461 tctccaccgg cttattagcg atagggatta tctgtaccgt gccaattatg gctcgctgga
     8521 aaaagctggt attgtggcaa aaaacacccg ttcataatac gcgctgataa tgatcaggag
     8581 tcacaccatg accattacgt ctctggatgg ctatcgctgg ctgaagaacg atattattcg
     8641 cggtaatttt caaccggatg aaaaattacg gatgagtttg ctgacatcgc gttatgcact
     8701 tggcgttggg ccgttgcggg aagcactttc tcaactggtg gcggaacggc tggtcacggt
     8761 ggtgaatcaa aaagggtatc gggtggcgtc tatgtcagag caggagctgc tcgatatttt
     8821 cgatgcccgc gccaatatgg aagcgatgtt agtgagtctg gcgattgccc gcggtggcga
     8881 tgagtgggag gcagacgttc tcgcaaaagc gcatctgctc agtaagctgg aagcctgtga
     8941 cgccagcgag aaaatgcttg atgagtggga tctgcgtcat caggcgtttc atacagcaat
     9001 tgtggcgggc tgtggttctc actatttgct gcaaatgcgt gaacggttgt ttgatctggc
     9061 ggcgcgctat cgatttatct ggctgcggcg aacggtgctt tcggtagaaa tgctggagga
     9121 taaacacgat cagcaccaga ccctgactgc ggcggtactg gcgcgagata ccgcgcgcgc
     9181 cagtgagtta atgcgccagc atttactgac gccaattccc attatccagc aggcgatggc
     9241 tggcaattaa ctactcttcc ggaatacgca acacttgccc cggataaatt ttatccgggc
     9301 tttttagcat cggtttattc gcttcgaaga ttttattgta cagattagcg ttaccgtaga
     9361 cctgtttgga aatggcactc agagtgtcgc cagacttaac ggtataaaac tggctggcag
     9421 tggctggtgt cgccgttttc acctgatcat caacactggc aataccggaa atattcccca
     9481 ccgcaacaag gattttctcc ttcgcctcct gactcaggcc gtcaccagtg accgtcgctt
     9541 tgccgtcggc aatttgaatg ttcactttat cagcatcagg tataccggtt ttgctcagat
     9601 gctcctgcac cttcttcgcc tgatcgtctt tatcgtgctg acctgtaacc gcgtcccaga
     9661 gtttttctcc ggcatctttc acaaaattga acagacccat agctacctca ttcgttaacg
     9721 gaaacactca gaaagtgtag cagaggaacg cggcgcgtta acgccgcggg ggtgagcaca
     9781 ggtttaatcg cgggtttgca cccagaacgc gtgaatcaaa ccggggatat acccaagcag
     9841 tgtcagcaga atattaataa tgaacgccca accgaaccct ttaccgagca gcacgccgag
     9901 cggcggtaga atgatggtga tgacgattct ccagaaaccc atatgtactc cctataagaa
     9961 aattactcat tgtttaaaaa gagattttat ctcttaagcg tagtaatttt agcggagcct
    10021 gccagtttca gcttacgctt ttactctcat ttacgctgct gaggctggta tatgttgcat
    10081 ttttagttta gagcttactt aatttagaaa atacttaaat aaatatgact gaactcgcgc
    10141 aattacaggc cagtgccgaa caggcagcgg ccttattgaa agcaatgagc catcctaaac
    10201 gattgctgat tctgtgcatg cttagcggtt cccccggcac cagcgcggga gagctgacgc
    10261 gcattaccgg actgagtgcc tctgcgacat cacagcatct cgctcgtatg cgggacgaag
    10321 ggcttatcga cagccaacgg gatgcccaac gcattctata ttccattaaa aatgaggcgg
    10381 taaatgccat tatcgccacc ctgaaaaatg tctattgtcc gtaaggagtc actatggctt
    10441 tgacaaccat ttcgccgcat gatgcacaag aattaatcgc acgcggcgca aagttaatcg
    10501 atattcgtga cgctgatgaa tatcttcgtg aacatattcc tgaagcagat ctggctccat
    10561 tatccgtgct ggaacagtca ggtcttccac ctaaattacg tcgcgagcaa attatattcc
    10621 actgccaggc aggtaaacgc accagtaata acgccgataa attagcagcg attgccgccc
    10681 ccgcagaaat atttttactc gaagatggga ttgatggctg gaaaagagct ggattgccag
    10741 tagcggtaaa taaatctcaa cccttgccgt taatgcgcca ggtgcagatc gctgcgggtg
    10801 gtttaatatt aatcggcgtt gtactgggtt ataccgtaaa tagcggtttc ttcttattaa
    10861 gtggctttgt tggtgccggg ttactgtttg caggaatcag cggtttttgt ggaatggcaa
    10921 ggttgttaga taagatgccg tggaaccaac gagcttgaga agcgacgccg gacgcgccct
    10981 agcagcgaca tccggcctca gtaattagat caggaaatcg tcgagagatt taccttctgc
    11041 cagcgcctga gcaattggct tcggtgtacg gccctgaccg gtccaggttt tagtttcacc
    11101 gttaacatcg gtgaatttat atttcgccgg acgcggctgg cgttttttac cagcgcgtgg
    11161 tgcagcagca gagctattac ccaataactc ttccgggtta attccgtcag ccttcatcag
    11221 ctccagccag gtgctaattt tttcctgacg ctcagccagt tcacgctgct gctgttcttc
    11281 ttcttcacgt ctttctttag tgacaaccct gaatttttcg agcatttctt caagaacgtc
    11341 aatggagaat tcgcgagcca tcgcacggag ggtgcgaatg ttatttaaac tttgtaacat
    11401 tacggacata ataaagaaaa ccttttaacg ccaaaacaaa aaagtatttc gtcgcgtagt
    11461 gtaagcgatc acttattata tttccacaaa atttaatcta tttacagtct tgcgcgagat
    11521 tatttcagct gttattttct gaaactctca gaataatttc aagattatat attagccatt
    11581 ttgatgagta atttagatta ttggtgaaca gaacggcgct ctctgctcgc taattccgca
    11641 agcctgacaa aaccgaagtg aataaaaatg caatataaca gaataaaaga
//
LOCUS       DAJVRF010000123        11500 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJVRF010000123 DAJVRF010000000
VERSION     DAJVRF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11500
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.123"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..487
                     /locus_tag="OQN13_003105"
     CDS             <1..487
                     /locus_tag="OQN13_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1616392.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
     gene            539..1474
                     /gene="ttcA"
                     /locus_tag="OQN13_003106"
     CDS             539..1474
                     /gene="ttcA"
                     /locus_tag="OQN13_003106"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCS1616393.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1603..2976)
                     /gene="dbpA"
                     /locus_tag="OQN13_003107"
     CDS             complement(1603..2976)
                     /gene="dbpA"
                     /locus_tag="OQN13_003107"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCS1616394.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3454..4437)
                     /gene="zntB"
                     /locus_tag="OQN13_003108"
     CDS             complement(3454..4437)
                     /gene="zntB"
                     /locus_tag="OQN13_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCS1616395.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4692..5924
                     /gene="dgcM"
                     /locus_tag="OQN13_003109"
     CDS             4692..5924
                     /gene="dgcM"
                     /locus_tag="OQN13_003109"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCS1616396.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5945..6508)
                     /gene="smrA"
                     /locus_tag="OQN13_003110"
     CDS             complement(5945..6508)
                     /gene="smrA"
                     /locus_tag="OQN13_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCS1616397.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6838..7746)
                     /locus_tag="OQN13_003111"
     CDS             complement(6838..7746)
                     /locus_tag="OQN13_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1616398.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7922..9232
                     /gene="abgA"
                     /locus_tag="OQN13_003112"
     CDS             7922..9232
                     /gene="abgA"
                     /locus_tag="OQN13_003112"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HCS1616399.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAVTASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9232..10677
                     /gene="abgB"
                     /locus_tag="OQN13_003113"
     CDS             9232..10677
                     /gene="abgB"
                     /locus_tag="OQN13_003113"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCS1616400.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10714..>11500
                     /locus_tag="OQN13_003114"
     CDS             10714..>11500
                     /locus_tag="OQN13_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCS1616401.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMLPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGL"
BASE COUNT         2939 a         2927 c         2855 g         2779 t
ORIGIN      
        1 gaatgtcaga agaaacctga cgatacttga tatgttcggt cccccaaaaa caaatgccgg
       61 gatccggacg gtaacattac tgcagccggc tcttgaagca ctgaaggagc aatacaaact
      121 gaccgggcat catcgcaaaa gcgaaatcac tttttatcat cgggagtacg gcagaactga
      181 aaagcaaaaa ctgcattttg ttttcatgcc cagggtgtgt aacgaaaaac agaaacctta
      241 ttactcggta agcagtttgg gtgcaagatg gaatgcagca gtaaaacgtg ctggtattcg
      301 ccgccgtaat ccgtaccata cgcgacatac ttttgcctgc tggctgttga cggcaggagc
      361 gaacccggca tttatagcca gccagatggg gcatgaaact gcgcaaatgg tgtatgaaat
      421 ttacggtatg tggattgatg acatgaacga cgaacagata gccatgttga atgcgcggtt
      481 atcgtagttg caaagtttgc ccccaatttg ccccatttag taccagagaa ctgaaataat
      541 gcaagaaaat caacaaatta caaagaaaga acaatacaac ctgaacaaat tacaaaaacg
      601 tctgcgtcgt aacgtgggcg aagccattgc tgacttcaat atgattgaag aaggcgatcg
      661 catcatggtt tgcctctccg ggggtaaaga cagctatacc atgctggaga ttctgcgcaa
      721 tttgcagcaa agcgcgccaa tcaatttttc gctggtggct gttaacctcg atcaaaagca
      781 accgggcttc ccggaacacg ttctgcccga gtatcttgaa aagctgggcg ttgagtacaa
      841 gattgtcgaa gagaacactt acggtatcgt gaaagagaag attccagagg gcaaaaccac
      901 ttgctcactg tgttctcgcc ttcgtcgcgg tatcctttat cgtaccgcaa cggaactggg
      961 ggcgacgaag atcgcgctgg gtcaccatcg tgacgatatc ctgcagacgc tgttcttaaa
     1021 tatgttctac ggcggcaaga tgaaaggtat gcctccgaaa ctgatgagcg atgatggcaa
     1081 acatatcgtt attcgtccgc tggcctactg ccgcgagaaa gatattcagc gatttgccga
     1141 tgcaaaagcg ttcccgatta ttccgtgcaa cctgtgcggt tcacagccta acctgcaacg
     1201 tcaggtgatt gctgacatgt tgcgtgactg ggataaacgt tatccagggc gtatcgagac
     1261 gatgttcagc gcgatgcaga atgtggtgcc gtcgcatctg tgcgatacca acctgttcga
     1321 tttcaaaggc atcacccacg gttctgaagt ggttaacggg ggtgatctgg cgtttgatcg
     1381 cgaagagatc ccactacaac cggcgggctg gcagccagaa gaagatgaaa atcagttgga
     1441 tgagttacgg ctgaatgtgg ttgaagtgaa ataaccagga tggcgcccga tgcgcaagcg
     1501 tatcgggcta ctcttatgga ggccggataa gacgcggcca gcgtcgcatc cggcaatccc
     1561 gaataagatg tttactcttg cacccggcaa ttcaacattt cattatttta ataaccgcac
     1621 ccggctcgtt tttcctttaa tcttcccgcc ctgtaactgt ttccatgctt tatgagcaac
     1681 agcctgacgg accgcgacat agacatgcgc cggatgcacg gcgattttgc caatatctgc
     1741 gccatcaagc ccgatatctc ctgtcagtgc acctaataca tcacccgggc gcattttggc
     1801 ttttttccca ccatcgatac acaacgttgc catttctgct tccagcggca caatggaact
     1861 attagctggc ggcgtttgcc agttaagttt tatctgcaac atgtcagaaa tgatattggc
     1921 ccgctgtgct tcttccggag cacagaaact gatcgccaga ccgctatttc ctgcacgagc
     1981 tgtacggccg atgcgatgta catgaacttc agggtcccac gccagctcaa agttcaccac
     2041 cagctcaagc gatttaatat ccagaccacg cgcagcaaca tcagtcgcaa ccagtacacg
     2101 ggcgctaccg ttagcaaaac gtaccagggt ctgatcgcga tcgcgttgct ccaaatcgcc
     2161 gtgtaatgac aatgcacttt gccctacttc attcagcgcg tcgcagacag cctggcaatc
     2221 ttttttggta ttgcaaaaca ccacgcaaga ggatggctga tgcaagctta ataaccgttg
     2281 caacagagga attttgcctt tgctggatgt ctcataaaat tgttgttcaa tgggtggcaa
     2341 agcatctgtt gagtcaattt caatcgccaa aggatcgcgt tgcactcgtc cgctgattac
     2401 ggcaatggct tccggccagg ttgccgaaaa cagaagcgtc tgtcgagatg caggcgcaaa
     2461 acggatgaca tcatcaatgg catcgctaaa tcccatatcc agcatgcggt cggcctcatc
     2521 catcaccaaa gtattcagcg catccagtga taccgtgcct ttttgcaagt gatccagcaa
     2581 acgacccggc gttgccacga taatatgcgg cgcatgttgc aacgaatcac gctgcatacc
     2641 gaacggttga ccaccgcaca acgtcaaaat tttggtattt ggcagaaaac gcgccagccg
     2701 acgcaattca cctgccacct gatccgccag ttcacgcgta ggacacagca ctaaagcctg
     2761 ggtttgaaat agcgacgcat caatttgctg taacaagccg aggccaaaag ccgccgtttt
     2821 gccgctgccg gttttcgcct gcacgcgaac atcttttccg gcaaggatcg ccggaagcgc
     2881 ggcggcctgc accggcgtca tagttaaata acccaactca ttaaggttcg tgagttgggc
     2941 gggaggcaaa acattcaggg tagaaaaagc ggtcacaatc tattctcgtg gtcatcgacg
     3001 caaagttagc aggcgcgtat cctcgcagat ctacgctcac gatgcgacaa tttaatcggt
     3061 tcttcatcgg gtggtgggtc aggcatgggt tgcgggcgag ggatcggatc gggcactgga
     3121 acaggatcgc caggaatcgg ttcagggaca ggaatttgca aataaataag tgtcgtcata
     3181 tttccctccg gtcattgggt ggactcttaa agggtagacg ctgataaata acaggcaaaa
     3241 aaaagccgac tcatcaaagt cggcgtcgta cgaatcaatt gtgctatgca gtaattcaaa
     3301 aaaggaagta agacaatatg gagcgcaacg cccatcgctt gacgttgcat tcacctgcaa
     3361 gggagatatt gccctgaatg ggtagagagt ttattgactt cgctcaaact ttgcggcgtt
     3421 tttgtataca gacagccgga aaaattgctt ttgttacaac catttactac ggtgcaacca
     3481 taaagcaaca ccaccaataa gaacaactaa cagaatacaa aaaattgaaa atccgaattg
     3541 ccacccgccg ccagggatcc caccaaggtt gacgccaaat aacccggtca gaaaggtact
     3601 gggtaaaaag accattgcca tcaacgacat tgtataggta cgacgagcta aattttcctg
     3661 catcacctga gcgatttcat ccgccatcac gccagtccgt gctatacagg cgtcgatttc
     3721 gtcaaggccg cgcccaaggc gatcggcaat atcctgcatc cgacggcgtt ggtcatcgct
     3781 catccacggc aaacgttcac tggcaagacg agcataaaca tcacgttgcg gtgccatata
     3841 gcgacgcatc acaattaatt gtttgcgcag cagagccagg aatccacgcg gtggaatttg
     3901 ctgatcaagg agattatctt caaggtcgat aattttatcg tgcagctgct cgataaattc
     3961 gctggaatga tcggtcaacg catcgcacac atccaccagc caccctccgc aatcggtcgg
     4021 acccgtgccc tcttccagat cgctcaccac atcgtccagc gccagcactt tgcgttgtcg
     4081 ggtcgaaaca attaaccgcc cgtccatata tacacgcatg gcgaccagtt gatcggggcg
     4141 ttcatcggtg ctgccgttta tacagcgcaa tgtaatcagc gtgccttcac cgagacggct
     4201 gactcggggc cgcgtgctct cgcccgccag cgcatcacgt acgttattgg gaagcagcgg
     4261 tgttgtcgcc agccattggg cgctatcatg gtgtacataa ttaaggtgga gccagcaggg
     4321 atgcgcttca tcaatcacat ctgtattttc cagcggttta acgccgcctc taccatccag
     4381 catccaggca aatactgcat ccgggacatt aacgtccgat cccttaatcg cttccacagt
     4441 gcctccatca tcaacgcatt attttgtagt ctagccttct ggccctgtta cgcaacatct
     4501 catcacccca ttaccctgaa atgattaata aaattctgtc taaattgaat acaaaaagca
     4561 aaatgctttt ccgtatacaa accgtgtgaa gtgttaaata gcgtctatca ttatcagaat
     4621 tatctgatca tatgacgtgg cttttttgcg atcggatagc aacaaaaatt gataaaaata
     4681 acgggatctc aatgattacg cacaacttca ataccctgga cttactcacc agtcctgtct
     4741 ggatcgtttc gccctttgag gaacagttaa tttatgccaa tagcgcggcg cgactgttga
     4801 tgcaagacct cacgtttagt cagctacgaa ccggtcccta ttccgtctcc tcacaaaaag
     4861 aactgccgaa atacctctcc gatctgcaaa accaacacga tattatcgaa atcctcactg
     4921 ttcagcgtaa agaagaggaa acagcattaa actgtcggct tgttttgcga aagctgacag
     4981 aagcagaacc ggtgattatt ttcgaaggta tcgaagcgtc ggcaacgctg ggtttaaaag
     5041 ccagtcgctc ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc ttttttctga
     5101 ctaactctgc accaatgttg ttgattgacc cctcacgaga tggccaaatc gtcgatgcta
     5161 acctcgccgc gctcaatttc tatggttata accatgaaac gatgtgccag aaacatacct
     5221 gggaaataaa tatgctcggg cgtcgcgtca tgcctatcat gcatgaaatt tcgcatttac
     5281 ccggtggtca taaacctctg aattttattc ataagctggc ggatggttcg actcgtcatg
     5341 tgcagaccta cgccgggccg attgaaattt atggcgacaa gctcatgtta tgtattgtac
     5401 atgatattac tgagcaaaaa cggctggagg agcagctgga acatgctgcc caccatgacg
     5461 cgatgaccgg attactgaat cggcgacagt tttatcacat taccgagcca ggccaaatgc
     5521 agcatctcgc catcgcccag gattacagct tgttgctcat cgacaccgat cgttttaaac
     5581 acattaacga tctctatggg cattctaaag gtgatgaggt gttatgcgcc ctcgcccgca
     5641 ccctcgaaag ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc gaagagtttg
     5701 tcttattgct accaagaacc ccactggata ccgcgctttc gctggctgaa actatccgcg
     5761 taagcgtggc aaaagtgagt atttcgggct taccacgctt taccgtcagc attggtgtgg
     5821 cgcatcacga aggaaatgaa agcatcgatg aactgtttaa acgcgttgat gatgctttgt
     5881 atcgggcgaa aaatgatgga cgcaaccgcg tgctggcggc ataagccgcg gatgcgtctc
     5941 gagatcaacg actgcgctta gcgtggcgct cccagttttc ttgcttcgcc tgcgccgttt
     6001 tacgtagtgc gacgtaacac gccccgctgc cgccatgatg cggtagcgca gtgcaatatg
     6061 cctgaacatc atcaaattcg gtcagccagc gcgccacata gctgcggaca atattggcat
     6121 gcgatttatc atcccgtcct ttgccatgaa taatcagcac gttacgcaaa ccatcagcca
     6181 tggcttgttg aatgaaactg aacatcattt tgcggcactc ttccaccggc tggcgcaaaa
     6241 gattcaggct cgcctgttgc ggatatttac cactgcgcag cttatccagc accccatgtt
     6301 gcaacccttc ccgccgaaac tccagcggct gacttagtgg gatgatgtcg agaaatccgg
     6361 tggtgaggaa attatcaagc tgcagcgtgt cgatacgctg cggcgcacgt tggttacgcg
     6421 ttggatgcca gtggacatcg gtagcacgtt tcagcggctg gacatcttcc atggcgtcaa
     6481 gaaacagcga tttgtcgtca aggttcatgt tacatcctcc cgcaattaag agcgcgatat
     6541 gataaccaga ccgggtcggt ccaacaacgt attacccaaa attccagtaa taagttccaa
     6601 atattgccga tattttaagc aaaatgctta tgcatgatta ttcattcacg atattaataa
     6661 tgtaacttat attttcgtga aatctgtcac tgaagaaaat tggcaactaa aggttaaaac
     6721 cgttataaca cagtcaccgg cgcagaggag acaatgccgg atttaagacg cggatgcact
     6781 gctgtgtgta ctgtagagtc tggcggatgt cgacagactc tattttttta tgcagtttta
     6841 actttgcaga tagccgcatt ctcgccggaa ttgcgtgatt aatgacgcgg tcagtggtgt
     6901 ctggcgacta tcacgccgct gaatcaaata ataggccgct ttcggtaaaa tttcgctaac
     6961 cggcaacatc accagcccct gtccgtgcaa gggatcgcag cccatttctt caggcagtat
     7021 gctgagaaaa tcgctttttg ccaccagact gatacaggct gagaacgtct cgcagactac
     7081 accgacctgt ggcgtttgcg cctgatcgtc aagcaattca ctcaactgtt tgtagtaact
     7141 gccgtgtggc gtcggcattg tccagctgta atccagtaac tgtttgatcg aacgggcacc
     7201 aatggcgggg tgtcccgggc ggcaaaagat cgcgaattgc ttttccagta atttctcaaa
     7261 agtaaattcg tggtcgtacg gtccctgata ataggtattg atggtgaaat ccaattctcc
     7321 ctgacgcaat tcattaatca tcgacaccag ttgcccttcc ataatgcgta cttttacctg
     7381 cggatgctgc tgatgaaaac gagatatgac agctggcatc agactgcggg aaatactggc
     7441 ccccatgccg atattaatct gccctgccag ttgcccttgt cgttggcgaa tatcctcttg
     7501 ggctgcgcgc agctcttcaa gaataagact ggcgtgctga taaaaacttt caccggcatc
     7561 agttaacgtc acgcctttac tacggcgaaa aaagagttgc gccgctaatc cttcttccag
     7621 ctcctgaata gatttactca gtgccggttg cgacatattc aacattcggc tcgctccgcg
     7681 aatgctgccc tgacgagcca cttcaacaaa agcccgaatt tgatgaattt ttacctgaaa
     7741 agccatgacg ccaccgataa ccgttattta tcagaccaaa gaaactggca tctactttaa
     7801 tgcagatgat tgtgtcaggg taatttatga acggttaaaa ctgtgaaaaa tcagttagtg
     7861 ataagtaaaa actatcgcta cgtgaaccgg gtcacacttt ttactgatga cgggaaaggt
     7921 tatggagtct ttgaatcaat ttgttaattc gcttgcccca aaattatcgc actggcgacg
     7981 tgattttcat cactatgcag agtctggctg ggtggaattc cgcactgcca cccttgttgc
     8041 ggaagaattg cagcagctcg gctattcact ggcgctgggc cgcgaagtag ttaatgaaag
     8101 tagccggatg ggactacctg atgaactcac tctacaacgc gaattcgagc gcgctcgtca
     8161 acagggtgcg ctagcacaat ggattgcggt ttttgaaggt ggtttcactg gtatcgtcgc
     8221 caccctggat accggtcgcc ccggtccggt gatggctttc cgtgtcgata tggacgcgct
     8281 ggatctcagt gaagagcagg atgtcagcca tcgcccctac cgcgacggtt ttgcgtcatg
     8341 taacgccgga atgatgcatg cctgtggtca tgatggacat accgccattg ggcttgggct
     8401 ggcgcatacc cttaaacaat tcgagtccgg actacatggc gtcatcaaac tgatttttca
     8461 gcctgcagag gaaggtacgc gtggcgcgcg ggcgatggtc gatgcaggtg tcgtagatga
     8521 tgttgattat tttactgccg tgcacattgg cactggcgta cctgcgggca ccgtggtgtg
     8581 cggcagtgat aattttatgg caaccaccaa atttgacgcg cacttcaccg gtaccgccgc
     8641 tcacgcaggc gcaaaaccag aagacggtca caatgccttg ttggcggcag cacaagccac
     8701 tcttgcactg catgcaatcg ccccgcacag cgaaggagct tccagagtaa acgtgggcgt
     8761 tatgcaggca ggaagcggtc gtaacgttgt tcctgcctcg gcgttgctga aagtggaaac
     8821 acgcggggcc agcgacgtca ttaatcaata tgtttttgac cgtgcacaac aagcgattca
     8881 gggcgcagca accatgtatg gtgtcggcgt tgaaactcgt ctgatgggtg cagttaccgc
     8941 cagttctcct tcgccgcaat gggtcgcatg gttgcaaagt caggcggctc aggtcgcggg
     9001 ggtcaatcag gccattgaac gtgttgaagc gcctgcgggt tccgaagatg ccacattaat
     9061 gatggcccgc gtgcagcaac atcaagggca agcctcctac gtggtgtttg gcacacagct
     9121 ggcggcaggt catcacaacg aaaaattcga ttttgacgag caggttctcg ctattgccgt
     9181 cgaaacgctg gcgcgcaccg cgctcaattt tccctggacg cgaggtatct gatgcaggaa
     9241 atctatcgtt ttatcgacga tgcgattgaa gccgatcgcc aacgttatac cgatattgcc
     9301 gatcaaatct gggatcatcc agaaacacgt tttgaagagt tctggtcagc ggagcatctg
     9361 gcttcggctc tggaatctgc aggcttcacc gttacccgca acgtaggcaa tatcccaaat
     9421 gcctttattg cttcgtttgg tcaaggcaaa ccggttatcg ccctgctggg agaatatgac
     9481 gccctggcag gtttaagtca gcaagctggt tgcgcgcaac ctacatccgc gacgcccggt
     9541 gaaaatggtc acggttgcgg acacaatttg cggggaaccg ccgcctttgc cgctgcaata
     9601 gccgtcaaga aatggctgga acaatatggg caaggcggca cagtgcgctt ttatggttgt
     9661 cctggcgaag aaggcggctc gggtaaaacg ttcatggtcc gcgagggggt atttgatgat
     9721 gtggatgcgg cactcacctg gcacccggaa gcctttgccg gtatgttcaa tacccgcacg
     9781 ctggcaaaca ttcaggcatc atggcgcttt aaagggatcg cagcacatgc cgcgaattcc
     9841 cctcatttgg gacgcagcgc ccttgatgcc gtaacgttga tgaccactgg caccaacttc
     9901 ctcaacgaac atattattga aaaagcgcgc gtacactatg ccatcacaaa tagcggcggg
     9961 atctcgccca acgtggtcca ggcgcaggca gaagtgcttt atcttatccg cgcccccgaa
    10021 atgaccgacg tgcagcatat ttatgatcgg gtcgccaaaa tcgccgaagg tgcggcattg
    10081 atgaccgaaa ccacggttga atgccgcttc gacaaagcct gttccagtta tctcccgaat
    10141 cgcaccttag aaaatgccat gtaccatgcc ctatcccatt ttggtacccc ggaatggaac
    10201 tccgaagaac tggcttttgc gaaacaaatt caggctacgc tcacccccaa cgatcggcaa
    10261 aacagtctga ataatatcgc cgcaaccggt ggcgaaaacg gcaaggcttt tgcactacgt
    10321 catcgtgaaa cggtactggc gaatgaggtc gctccatatg ccgccaccga taacgtgctt
    10381 gcggcatcga ctgatgtcgg cgacgttagt tggaaactgc ctgttgccca gtgtttcagc
    10441 ccctgttttg ccgtcggtac accgctacat acgtggcaac tggttagcca ggggcgaaca
    10501 tctattgctc ataaaggaat gctgctggcg gcgaaaacta tggcagcaac cacagtcaat
    10561 ctcttcattg attcagggct attgcaagaa tgccaacaag agcatcagca agtaacggac
    10621 acgcaaccgt atcactgccc tatcccgaaa aacgtgacac cgtcaccttt aaaataacaa
    10681 caacaacgca aacacaacaa ccgaggaatg cccatgagta tgtcatccat accgtcgtcc
    10741 tcccaatccg ggaagctcta tggctgggtc gaaagaattg gtaacaaggt tctccatcct
    10801 tttttgctct ttatctattt gattatcgta ctcatggtga cgacggcaat tttgtcggcc
    10861 tttggcgtca gtgcgaaaaa cccgaccgat ggaacgccgg tggtggtgaa aaacctgctc
    10921 agtgtggaag gattacactg gtctttaccc aatgttatta aaaactttag cggttttgct
    10981 ccacttggtg cgatcctggc gctggtttta ggtgccggtc tggcggagcg cgtcggctta
    11041 ctgccagcgc taatggttaa aatggcatcg catgttaatg cccgctacgc cagttatatg
    11101 gtgctgttta ttgctttttt cagccacatt tcttccgatg cgacgttagt gatcatgcta
    11161 ccgatgggtg cgctgatttt tctggcggtg ggcaggcatc cagttgcagg tttactggct
    11221 gccattgcag gcgtaggttg cggctttacg gctaatttac tgattgtcac aaccgacgtg
    11281 ttgctgtcgg ggatcagcac ggaagcggcg gctgcgttca atccgcaaat gcacgtcagt
    11341 gtaattgata actggtattt tatggccagc tccgtagtcg tactgacgat tgttggcggc
    11401 ctgataaccg acaaaatcat cgagccacgg ttaggtcaat ggcagggaaa cagcgatgag
    11461 aaactgcaga cattgaccga aagtcagcgt tttggtttac
//
LOCUS       DAJVRF010000124        11420 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJVRF010000124 DAJVRF010000000
VERSION     DAJVRF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.124"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="OQN13_003115"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="OQN13_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCS1616402.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="OQN13_003116"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="OQN13_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCS1616403.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="OQN13_003117"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="OQN13_003117"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCS1616404.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="OQN13_003118"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="OQN13_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCS1616405.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="OQN13_003119"
     CDS             complement(3928..5214)
                     /locus_tag="OQN13_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS1616406.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="OQN13_003120"
     CDS             complement(5235..6026)
                     /locus_tag="OQN13_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCS1616407.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="OQN13_003121"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="OQN13_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCS1616408.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="OQN13_003122"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="OQN13_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCS1616409.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="OQN13_003123"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="OQN13_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCS1616410.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="OQN13_003124"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="OQN13_003124"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCS1616411.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="OQN13_003125"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="OQN13_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCS1616412.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="OQN13_003126"
     CDS             complement(9882..10364)
                     /locus_tag="OQN13_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCS1616413.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11420)
                     /gene="dgcN"
                     /locus_tag="OQN13_003127"
     CDS             complement(10380..>11420)
                     /gene="dgcN"
                     /locus_tag="OQN13_003127"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HCS1616414.1"
                     /translation="SLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILASWH
                     YTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAVLT
                     GCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRFAL
                     DFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARKTS
                     ALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLYDV
                     QSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHNMY
                     QAKHQRAEKLVR"
BASE COUNT         2800 a         2746 c         3059 g         2815 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgtt ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccag ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat catcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggtggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggccc tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctact aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact
//
LOCUS       DAJVRF010000125        11308 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJVRF010000125 DAJVRF010000000
VERSION     DAJVRF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.125"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..208
                     /locus_tag="OQN13_003128"
     CDS             <1..208
                     /locus_tag="OQN13_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1616415.1"
                     /translation="IVPLTVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFA
                     QTTKGVLETGIYRGVVSIYLSQDI"
     gene            300..2984
                     /locus_tag="OQN13_003129"
     CDS             300..2984
                     /locus_tag="OQN13_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCS1616416.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLAHHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            2981..4069
                     /locus_tag="OQN13_003130"
     CDS             2981..4069
                     /locus_tag="OQN13_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCS1616417.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4152..5449)
                     /gene="exuT"
                     /locus_tag="OQN13_003131"
                     /pseudo
     CDS             complement(4152..5449)
                     /gene="exuT"
                     /locus_tag="OQN13_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            5931..7343
                     /gene="uxaC"
                     /locus_tag="OQN13_003132"
                     /pseudo
     CDS             5931..7343
                     /gene="uxaC"
                     /locus_tag="OQN13_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
     gene            7358..8845
                     /gene="uxaA"
                     /locus_tag="OQN13_003133"
     CDS             7358..8845
                     /gene="uxaA"
                     /locus_tag="OQN13_003133"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCS1616418.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTKVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINIRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8928..9479
                     /locus_tag="OQN13_003134"
     CDS             8928..9479
                     /locus_tag="OQN13_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCS1616419.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9484..10728)
                     /gene="sstT"
                     /locus_tag="OQN13_003135"
     CDS             complement(9484..10728)
                     /gene="sstT"
                     /locus_tag="OQN13_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCS1616420.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         2969 a         2772 c         2934 g         2633 t
ORIGIN      
        1 gattgttccg ctgacagtaa cctggggcgg tgaagaaatt aaagctgatg cggcaaccac
       61 ttttaccgca accaaaatct ttgcttccga tgcgttaact aacggttctc tggctaaaaa
      121 cctgatgttt gctcaaacca ccaaaggtgt tctggagaca ggcatctacc gtggtgtagt
      181 aagcatttat ctgtctcagg atatctaatc cagataacag aattaacacg taacaaaggg
      241 aggggtagcc ctcccttatt ttaactgttt tagtatttgt tatggatttc aggattgcca
      301 tggataaaaa attactggct cttttgatcc tggcgagtct cagcccggca gaggcgacat
      361 taaccgaaat tcccgcaggg tttgaggtta ttgctcaggg acagcaggag tatatcgagg
      421 tttatttttc agggaaaagt ctcggtaaat attatgcaat ggttaatctt gataccgtaa
      481 catttcttga tccagcaagt ctatataaca agctggaact ggatgtagac gatcagaaaa
      541 tcgcgcatat agtgaaagaa aaattatcgc agccgctagc tcaccacggt gaattggctt
      601 gcggttatgt acgtactgac tcagggtgtg gttttctgaa taccgatacg ctggaaataa
      661 tctataatga tgaagaaagt tcggcaacgt tgtttattaa tccgcaatgg aattcagctt
      721 tcgatgcgaa gtcattatat ttaaatccag acaaaaatac tgttaatgct tttatacatc
      781 agcaagacat caatgttctg gcacaggatg attaccaatc gttgtctatt cagggaaacg
      841 gtgcgctggg aataacagaa aatagttata ttggtgcaca ctggaatttc aacggttatg
      901 atgcagatga tgtcagtgac agtaatgctg acgtcagcga tctctattat cgttatgatt
      961 ttttacgtcg ttattatgtg caggcgggtc gcatggacaa ccgcacacta tttaatgcac
     1021 aaggcgggaa ctttaccttt aactttctgc cactcggtgc aattgatggg atgcgtatcg
     1081 ggtcgacact gagctattta aaccaggcgc aaagccagca aggaacccca gtaatggttc
     1141 tgctttcgcg caattctcgt gttgacgctt atcgtaatga gcaacttttg ggatcgtttt
     1201 atctcaatag tggttcgcaa tttattgata ccagttcctt tccgccaggc agctacagcg
     1261 tggcattaaa agtctacgaa aataaccaac tcacccgcac cgagcttgtg ccgtttacca
     1321 aaaccggcgg tctgactgac ggaaatgcgc aatggttctt acaggcaggt aaaactacat
     1381 cacaggtttc tgatgatgaa agttcagctt atcagctagg agtacgcctg ccattacatc
     1441 cgcaatatga gctctacgca gggctggcga atgccgatga tgtgagtgct ttcgagttag
     1501 gtaataactg gacggcagat ttaggcgggg tggggaatct tgcaatcagc gccagcgtgt
     1561 tccgtaacga tgacggcggc aaaggtgata tgcaacaggc caactggagt aatccgggat
     1621 ggccgacgtt gggcttttat cggaccaact ctgacggcga tgcttgtaca accgacagca
     1681 gagagagcta taacgcctta agctgttatg aaagtatttc cgcgacggtt tcacagaatt
     1741 ttgtcggctg gaatatgatg ctgggttata cccgcacaca aaataacact gatgatagtt
     1801 tgcgttggga taaacagcag agctttgaaa ataactatct tcgccagaca acagcgcaaa
     1861 gtatctctga aactgtacaa cttagcgctt cccgcgcttt tgtgatgcgt gactggattt
     1921 tgagtacgtc agtcggtgtt ttccatcgta atgacaacgg tggcgataac gacgacaacg
     1981 gcttgtactt atcgttttcg ttatctgaca cgccaacgat ggatagcaat aacaacagcc
     2041 attcaacaaa tgtgtctacg gattatcgtt atagcgaaca ggatggcgat caaacgtcat
     2101 ggcagttatc gcataccttt tataacgatt cattcagcca taaagaactt ggcgtaaccg
     2161 tcggaggcct gaacaccgat accataaaca gcgcggttaa cgggcgttgg gatgggcaat
     2221 acggaaatgt ctacgctacc gtatctgata gttatgaccg taagaatcat gatcatcttt
     2281 cggcctttac gggcacttat agctctacgc tggctgtcag ttgctatggc gttaatttgg
     2341 gagccagtgg tacagacgat ttgctgggtg cggtactggt ggatgtgaaa ggcttctctg
     2401 aacaggatga agagagtcag gatctgcaac tcgaagcgcg ggtggcaggc agccgaactt
     2461 tgcagcttgg tcaaagtgac agtgtgttgt tcccttatcc tggatttcag tctggttttg
     2521 ttgaggttaa cgacagtagc cagggcaatc agcaggggac aacaaacatc attaacggtg
     2581 cagggaatcg tgaattaatg ttgttgcctg gcaagctgcg ctatcgcgaa gtgtctgcca
     2641 gctttaacta caactatatc ggtcgtttat tactgccggc agcggtgaaa aaattcccga
     2701 ttgtggggct gaacagcgcc atgttactgg tagctgaaga tggaggattt acacttgaaa
     2761 ttaacggcag cgaaaaagag ctctatctgc tttccgggca gcaattcctt aagtgtccac
     2821 tgagtgttgt aaagaaacgc gccagcattc gttacagcgg agatgttact tgtagtgtgg
     2881 tgacttattc acaattaccg gagtcgattc aggttcaggc acagttgaaa cagcctaaat
     2941 tacgtggaaa cgttcagacg gcgcaaaggg aggttgcacc atgagaaatc gattgattgc
     3001 ggcgatattg ggcttgtttg gcacgctcac tggcgttcag gcagctcctg acgtgaccag
     3061 tgaaattacg tatgatttgg catctggtag agcggattat tacttctgga aggatgaagc
     3121 gtcggcagga aataatggat atatgtggta tgaatgttcg tatcctgacc tccaacaaac
     3181 ctgtacagct aatggaaata tatcgacagt acaaatctat ttaactgaac aacgcagtgg
     3241 gatgcgttgg ccggtaaaac tcaaaggatt taaaacagcc attgtaagta gcgatgaagc
     3301 gccgccagga tgcaaggggg gcaaagggct tcagacgaat cttaaggatt ctaatagatc
     3361 ttcatgtaca gaagatggtc aacattatta tatatacgat acaaagtttc ttacgctcta
     3421 ccttgagcag acagagatga agaatttgcc gattggtggc gtctggaagg ggaaagttaa
     3481 attacattcg aacagcccgg cccaggacta tttcgcaaat attaccctga atacgctcga
     3541 ccccaaccat attgacgtgt tcttcccgga gttcgcccac gccacgccaa gggtgcagtt
     3601 agacttgcat ccaacaggaa gcgttaacgg cagcaactac gcgcaagatc tgaccatgtt
     3661 ggacatgtgc ctgtacgatg gttttaacgg taatgccatc agttatgaaa tcatgctcaa
     3721 agatgaaggg cgaccagctg cagggcgcag agacggtgac ttctctatct atcgtcaagg
     3781 agggaccacc accgacgagg gagaacgcat tgattaccgg gtcaaaatgt acaacccgga
     3841 aaccggtggg caaattgatg tgcgcaataa tgaaaatatg gtctggaaca gcattaacct
     3901 gaaacgtgtg cgtccggtgg tactgcccgg tattcgctat gccgtgatgt gtgtgccgac
     3961 acccttgact ctggcagtcg ataaattcag cgtgatggat aaacaggccg ggtactacat
     4021 gggcaaattg tcagtaatct ttacgccttc cttgccaacc atcaattgac cccgcaggcc
     4081 agaaaaaact aaacaacgaa gccgcctgat aaggcggctt cgtcattaca acttacgtta
     4141 aacgttaaac gttaatgttg cggtgcggga tcgttatgtg tttcctgcgc cacctcaatc
     4201 gccggtttgt tctgcaatac ggtccagata accagcgcac ccagcaggtc aaatactgcc
     4261 agaactgcga acagcgggct gaagccgatg gtgtcagcca gtgcaccgac aaccagcgca
     4321 aacagcgtac ttgccagcca tgcggacatc ccggttaaac cgttcgccgt cgccacttcg
     4381 ttacgaccga acacgtcaga agagagcgta atcagcgcgc cagacagtgc ctggtgggca
     4441 aaaccaccga tacacagcag cataattgcg acatacgggt tggtgaacag accgatcata
     4501 cccgggccaa tcatcagcac agcacccagc gttacgacca tcttacggga aacgatcagg
     4561 ttcacaccaa accaacgctg gaacagcggc ggcaggtaac caccgaggat acaaccgagg
     4621 tcagcaaaca gcatcggcat ccaggcgaac atcgcgatct ctttcaggtt aaagccgtaa
     4681 actttaaaca tgaacagcgg gatccacgcg ttaaaagtac cccaggccgg ttctgccagg
     4741 aaacgcggca gtgcgatacc ccagaactga cggttacgca ggatctgacc aacggacatt
     4801 ttcttcgccg tgctcacctg gtgctgggct tcctgaccat taataatata gtcgcgttct
     4861 tcatcggtca gatgcttctg gtcgcgcgga tgtttataga aaatcagcca tgccatcgcc
     4921 cagataaagc tcaatgcacc ggagatgata aatgccatct gccagctgtg catcacgatt
     4981 gccataccac cagcggcggc gcaatcatcg caccaatcga agaacctacg ttaaagtagc
     5041 ctactgcgat ggaacgctct ttcgccggga accattcgga gctggctttc agacccgccg
     5101 ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc aacagccagg ccaccccagc
     5161 tacctgccag cgcggttgca ccacagaaca cggcccacag cacagcaaac attgcataac
     5221 cgattttcgt acccagcaca tccagtacat aacctgctac cggttgcatg accgtataag
     5281 cagcagaata ggctgcgatg atataggaat actgttgggt ggagatgttt aactcttcca
     5341 tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag gtaaccaagc acggtgccga
     5401 gcgtcaccag tgcgatcata taccaacgta accctttaat tttacgcatg taaaacctca
     5461 tcgttttgtt atatccgcag gaagcgcggc catcatcccg ccagaggctg aagatgttag
     5521 ttacgaccgg gtaaccgaaa aaaaaccggc acgccccgaa cgttgccatg aaagtttcgt
     5581 gctaattcgg cttccgtacc ggttaacccg attcatctaa caaatgaatg aatcggcaat
     5641 ttgcattccg tcgacttatg atttgcgacg gcagaaagat aacttgtcat acaactttaa
     5701 aaggtgagag ccatcacaaa tgtgcgaata tttgtaggga cattacctga cgacagcgag
     5761 gccagtactg gcgcggcctg cagcgagatt tatcactttg agagtaattt tttaactacg
     5821 tttattgatc taactcacga aaatatcttc ggactctgga aattggtgtg ataactttgt
     5881 cagcatcgca ccataagcaa gctagctcac tcgttgagag gaagacgaaa atgactccgt
     5941 ttatgactga agatttcctg ttagataccg aatttgcccg ccgtctgtat cacgactacg
     6001 caaaagacca gccgattttc gattaccatt gccatttgcc gccgcagcag attgcggaag
     6061 actatcgttt taaaaacctg tatgacatct ggctgaaagg cgatcactac aaatggcgcg
     6121 ctatgcgtac caacggtgtg gccgagcgtc tgtgtaccgg tgatgcatct gaccgtgaaa
     6181 aatttgacgc ctgggcggcg actgttccgc acactatcgg caacccgtta taccactgga
     6241 cgcaccttga actgcgtcgt ccgtttggta tcactggcaa attgctttct ccgtcaactg
     6301 ccgatgaaat ctggaacgaa tgtaacgaac tgctggcgca ggataacttc tccgcgcgcg
     6361 gcatcatgca gcagatgaac gtgaaaatgg tcggcaccac cgatgacccg atcgattctc
     6421 tggagcatca cgcagagatc gccaaagacg gctctttcac catcaaagtg ctgccgagct
     6481 ggcgtccgga caaagccttc aacatcgaac aggcgacctt taacgactac atggcgaagc
     6541 tgggcgaagt ttccgatacc gacattcgcc gctttgctga cctgcaaact gccttgacca
     6601 aacgtctgga tcacttcgcc gctcacggct gtaaagtgtc tgaccatgcg ctggacgtgg
     6661 tgatgtttgc tgaagcgaac gaagcggaac tggacagcat tctggcgcgc cgtctggctg
     6721 gcgaaaccct gagcgagcac gaagtggcac agttcaaaac tgcggtactg gtgttccttg
     6781 gtgccgaata tgcacgtcgc ggctgggtac agcagtacca cattggcgcg ctgcgtaata
     6841 acaacctgcg tcagttcaaa ctgctggggc cggatgtagg ctttgactcc atcaacgacc
     6901 gtccgatggc ggaagagttg tctaagctgc tgagcaagca gaacgaagaa aacctgctgc
     6961 cgaaaaccat tctctactgc ctgaacccgc gcgataacga agtgctgggc accatgatcg
     7021 gtaacttcta gggcgaaggt atgccgggca aaatgcagtt cggttccggc tggtggttta
     7081 acgatcagaa agacggtatg gaacgtcaga tgacccaact ggcgcagctc ggtctgctga
     7141 gccgctttgt cggtatgctg actgacagcc gtagcttcct gtcatacacc cgtcacgaat
     7201 acttccgccg cattctgtgc cagatgatcg gtcgctgggt ggaagcgggc gaagcaccgg
     7261 cggacatcaa cctgctgggc gagatggtga aaaatatttg ctttaacaat gcgcgtgact
     7321 acttcgccat tgaactgaac taaggtctgg ggttgatatg caatacatca agatccatgc
     7381 gctggataac gtcgcggtcg ctttagcaga tttggctgaa ggcacaaaag tcagtgtcga
     7441 taaccagact gttacgctgc gccaggatgt tgctcgtgga cataaatttg cgttaacgga
     7501 tatcgcaaaa ggggccaacg tcatcaaata tggcctgccg attggttatg cattggcgga
     7561 tattgcggcg ggggaacacg tccacgccca caatacgcgc acgaatctga gcgatctgga
     7621 tcagtatcgc tatcaacctg attttcagga tctgcctgcg caagcggcag atcgtgaagt
     7681 gcagatctat cgtcgcgcta acggcgatgt cggggtgcgt aacgagctgt ggatcctgcc
     7741 aaccgtgggc tgtgtcaacg gcatcgcgcg gcaaatccag aaccgtttcc tgaaagagac
     7801 caacaacgcc gaaggtaccg acggcgtgtt cctcttcagc cacacctacg gctgctcaca
     7861 actgggcgac gatcacatca acatccgcac catgctgcaa aacatggtgc gccacccgaa
     7921 cgcgggcgca gtgctggtga ttggtctggg ctgtgaaaac aaccaggttg ccgcattccg
     7981 tgaaacgttg ggcgatatcg atcctgaacg cgttcatttc atgatctgcc aacagcagga
     8041 tgatgagatc gaagccggga tcgagcattt gcatcagctg tataacgtga tgcgcaacga
     8101 taaacgcgag ccaggcaaac tcagcgaact gaagtttggt ctggagtgcg gtggttctga
     8161 cggtctttct ggtattactg ctaacccgat gctggggcgt ttctctgact acgtgattgc
     8221 taacggcggt actaccgtac tgaccgaagt gccggagatg tttggcgcag agcagttgct
     8281 gatggaccat tgccgcgacg aagcaacgtt tgaaaaactg gtcaccatgg tcaacgactt
     8341 taaacagtac tttattgccc atgatcagcc gatctacgaa aacccatcac cggggaacaa
     8401 agcgggcggt atcaccacgc tggaagacaa atcacttggc tgtacccaga aagcgggttc
     8461 cagtgtcgtg gttgacgtgc tgcgttacgg cgagcgtctg aaaacgcctg ggctgaactt
     8521 gttaagtgcg ccgggtaacg atgccgtagc gaccagcgcc ctggcgggtg cgggctgcca
     8581 tatggtgctg ttcagtactg gtcgcggcac gccatacggt ggatttgtgc cgacggtgaa
     8641 aatcgccacc aacagtgaac tggcggcgaa gaaaaaacac tggatcgact ttgacgcggg
     8701 tcagctgatc cacggtaaag cgatgccgca gttgctggaa gaatttatcg acaccatcgt
     8761 tgagtttgcc aacggtaagc aaacttgcaa cgagcgtaac gacttccgcg aactggcgat
     8821 cttcaaaagc ggcgtaacgc tataaagtcg aaatgagttg ttaataaaaa acggcgtttc
     8881 ataatgtgaa gcgccgtttg cttttccata cctgataagg atttatcatg accgcgtatt
     8941 ggctggccca gggcgtgggt gtcatcgcct ttctgattgg tatcacaaca tttttcaatc
     9001 gtgacgaacg tcgcttcaaa aagcagcttt cggtctatag cgccgttatt ggcgtacatt
     9061 tttttcttct gggcacctat cccgctggtg ccagtgccat ccttaatgcc attcgtacat
     9121 tgattacctt acgcacgcgc agcttatggg taatggcgat ttttattgtg ctgactggcg
     9181 gaattggcct cgcgaagttc catcatcctg tcgaactatt gccggttatc ggtacgattg
     9241 tcagtacctg ggcgctgttc cgctgtaaag ggctcaccat gcgctgcgtg atgtggtttt
     9301 caacgtgttg ctgggtgatt cacaacttct gggcggggtc gataggcggc acaatgattg
     9361 agggtagttt tctgcttatg aatggcttga atatcattcg tttctggcgg atgcagaaaa
     9421 ggggaattga tccgtttaaa gtagagaaaa cccctcccgc cgtagacgaa aggggttaaa
     9481 caattaatta cgcagggcgc tatttgccag acgatcgtct tccgcctggc aagctgccgc
     9541 agtgaacagc acgtcagttg aagagttcag cgcggtttcg caagagtcct gcaatacgcc
     9601 gatgataaag ccgacggcaa ccacctgcat ggcgatatcg ttcgaaatac cgaacatatt
     9661 acaggccagc gggatcagca gcagagaacc ccccgccacg ccggatgcgc cacaggcaca
     9721 cagagaagcc accacgctca acagcagcgc cgtgggcaga tcgaccggga tacccagcgt
     9781 attaaccgca gccagcgtca acacggtaat ggtgattgct gcgcccgcca tattgatggt
     9841 ggcacccagc ggaatagaaa cggaataggt atcgcgatcc agattcagct tttcacacag
     9901 tgccatattc accggaatgt tcgccgcaga gctgcgggtg aagaaggcat acacgccgct
     9961 ttcgcgcagg cacagcagca ccagcgggaa cgggttacga cgaattttcc accacaccag
    10021 caatgggtta accaccagcg ccaccagtaa catacagcca accagcacga ccagcagttg
    10081 cgcgtagccc cacagtgtgg agaaaccggt ggttgccagg gtagaagaaa ccagaccaaa
    10141 aataccaatc ggtgcgaagc gaatgaccag tttcaccata aaggtgacgg cattcgacat
    10201 atcgttgacc aggtttttgg tggtctcgtt accgtgacgc agtgcgaaac cgaggccaat
    10261 tgcccacacc aggatcccga tgtagttacc tttcagcagc gcgtcgatgg ggttggaaac
    10321 catgctcatt accagcccgc gcatcacttc tacaatgcct gacggtggcg aaatatcacc
    10381 cgcgctactg gataagtgca gggtagaagg gaaggcgaag ctgaaaacga ctgcggccag
    10441 agcagcagag aaggtaccca gcagatagag gaacaaaata gggcggatat tggttttctg
    10501 cccatgctgg tggttagcaa tagatgccat caccagcatc aacaccagga tgggggcaac
    10561 ggctttcagt gcgccgacga acaaagtacc taacagacca acagcttccg ccgcgggttt
    10621 tgatatccat gccagaagaa tccccagaac aaggccgacc aggatttgtt ttaccaggct
    10681 gccatgagcc agacgccgga atagccccgg tgaacgttgc gtagtcattt ttcgatcctt
    10741 tcattgtgtt atcgcacatc cctggtgcgt tctgtcgacg catctttcag gatgtaacaa
    10801 agtgtttgca aggtcgagta taaggaaagt tacagcttcg ggaagcgaaa aatgctggat
    10861 tttaaagaga agtcatattt tgaaacttaa tggttacatt tgtttgacat aaacagaggg
    10921 aaggactggc tactaaggct gattagataa catatgggca aaactatttg gaatatgaga
    10981 acggttgcgc tcctttaatg tgctatgcca tataaagtca ctggcgcagg gagcgcgcag
    11041 ggggcggcca atcgccgccg ccccctgcac ccccaggctc tggcgaacaa aatcgccgct
    11101 gcgcgattcc ctcagcttat cccttgcggc taccgggtcg ggcgcaaggg aacgtccctg
    11161 taagatgcgc cctcgccgcg catccatgcg cgtcgccccg gcctccaggg aacgcttcgg
    11221 cgattttgac gccaccaaca cccgtgcggt ctttttgtaa agagctacaa gccgattaca
    11281 taattaatag ctcacctttg tctggaat
//
LOCUS       DAJVRF010000126        11233 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJVRF010000126 DAJVRF010000000
VERSION     DAJVRF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11233
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.126"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..309
                     /locus_tag="OQN13_003136"
     CDS             <1..309
                     /locus_tag="OQN13_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCS1616421.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN
                     "
     gene            508..789
                     /gene="ppiC"
                     /locus_tag="OQN13_003137"
     CDS             508..789
                     /gene="ppiC"
                     /locus_tag="OQN13_003137"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCS1616422.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="OQN13_003138"
     CDS             complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="OQN13_003138"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCS1616423.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2501..3394
                     /gene="ilvY"
                     /locus_tag="OQN13_003139"
     CDS             2501..3394
                     /gene="ilvY"
                     /locus_tag="OQN13_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCS1616424.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="OQN13_003140"
     CDS             complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="OQN13_003140"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCS1616425.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="OQN13_003141"
     CDS             complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="OQN13_003141"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCS1616426.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="OQN13_003142"
     CDS             complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="OQN13_003142"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCS1616427.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="OQN13_003143"
     CDS             complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="OQN13_003143"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCS1616428.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8117..9763)
                     /locus_tag="OQN13_003144"
     CDS             complement(8117..9763)
                     /locus_tag="OQN13_003144"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCS1616429.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9766..9816)
                     /locus_tag="OQN13_003145"
     CDS             complement(9766..9816)
                     /locus_tag="OQN13_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616430.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="OQN13_003146"
     CDS             complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="OQN13_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCS1616431.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10354..11233
                     /locus_tag="OQN13_003147"
                     /pseudo
     CDS             10354..11233
                     /locus_tag="OQN13_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
BASE COUNT         2551 a         3087 c         2948 g         2647 t
ORIGIN      
        1 gtcgtgctca acggaaaaat taatggcaac gcatgtatcg tcgttccatt atcaaccact
       61 cgcgatcatg acaagttaaa tcgtggaatg catgttgaaa tagccagtaa tgttatcaat
      121 gacctgcaat tctttgatca gcagattcgt tgggccaaag ccgacctagt acaacaagtc
      181 agtcgaaacc gattaaacag agcaaggact tacagagggt atcttaatca atgtttgcca
      241 catgagttgg ttgcagacat acagagggcg gtcatcaagt caatcaatgc catatcgctc
      301 ataaattgac aataaaataa ccatccccga taatgacttt gtagcccata gaggccctaa
      361 gttttctccc cttgagggac ctgcgaagcc cggattttcc gggcttttgc catatatgta
      421 ccagcccctc cctgactaac aattacgtta gcatctgcgg atcatgctaa aatcgccgcc
      481 tgacaattat tcactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt
      541 gtaaaagaag agaaactggc tctggatctt ctcgagcaga ttaagaacgg tgccgatttc
      601 ggcaagctgg cgaagaaaca ctccatttgc ccatcaggca aacgcggcgg tgatttaggt
      661 gaattccgcc agggtcagat ggttccggcg tttgataaag ttgtgttctc ttgtccggta
      721 ctggagccaa ccggcccgct gcacacccag ttcggttacc acatcattaa ggtgctgtac
      781 cgtaactaat agcaaggcct tctccaggag aaggccttga gtgttttctc cctctccctg
      841 tgggagaggg tcggggtgag ggcatcagcg cgcacttaac ccgcaactgc aatacgtttc
      901 atatccgtca tatagccgcg cagtttctta cctacctgct caatcgcatg gcagcgaatc
      961 gcttcgttca catcacgcag ttgcgcgtta tctaccgcac cttccggaat agctttaccc
     1021 agatcgcccg gttgcagctc tgccataaac ggtttcagca gcggcacaca agcgtaagag
     1081 aacagatagt taccgtactc ggcggtatca gagataacca cgttcatttc gtacagacgc
     1141 ttacgggcga tggtgttggc aatcagcggc agttcgtgca gtgattcata gtaagcagat
     1201 tcttcgatga tgccggaatc aaccatggtt tcaaacgcca gctcaacgcc cgctttcacc
     1261 atcgcgatca tcagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac
     1321 tgcggcgcgg tttcgaaggc ggttttgccg gtctcttcac gccaggtcag cagtttctta
     1381 tcatcgttgg cccagtccgc catcataccg gaggagaatt cgccggagat gatgtcgtcc
     1441 atatgtttct ggaacagggg tgccatgatc tctttcagct gttcagacag cgcgtaagca
     1501 cgcagtttcg ccgggttaga gagacggtcc atcatcaggg tgatgccgcc ctgcttcagc
     1561 gcttcggtga tggtttccca accgaactga attagttttt ctgcgtatgc cggatcggtg
     1621 ccctcttcca ccagcttgtc gaagcacagc agagagccag cctgcaacat accgcacagg
     1681 atggtttgct cacccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcacg
     1741 cccgcacggt gaccaccggt tgctgccgcc catgctttag cgatcgccat gccttcgcct
     1801 ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtac
     1861 tcttcacgta cttcggtgcc agggcatttc ggcgcaacca tgactacggt aatgtcttta
     1921 cggatctgct cgcccacttc gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg
     1981 tctttcatca gtggctgtac ggtgcgcact acatcagagt gctgcttgtc tggcgtcagg
     2041 ttaaccacca gatccgcctg cgggatcagt tcttcgtaag taccaacttt aaaaccgttt
     2101 tcggtcgctt tacgccagga cgcgcgcttc tcggcaatcg cttctttacg cagagcgtag
     2161 gagatatcga gaccagaatc acgcatgttc aggccctggt tcagcccctg tgcgccacag
     2221 ccgacgataa ccactttttt accctgaagg tagctcgcgc cgtcggcgaa ttcgtcgcgc
     2281 cccataaagc gacatttgcc cagctgtgcc agctgctggc gcaggttcag tgtattgaag
     2341 tagttagcca tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg
     2401 agattgaatt cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca
     2461 cattgcaatt tctgcaacgt caacatcgag ggctgcgcca ttggatttac gcgatctgaa
     2521 aaccttcctg catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt
     2581 tagcccatcc acgctctcac ggcagattca gcgcctggaa gaagatcttg gtcagccgct
     2641 atttgtgcgc gataaccgca cggtaacgct gacggaagcg ggcgaagagc tgcgcgtttt
     2701 cgcccagcaa acgctgttgc agtatcagca attgcgccac accatcgatc agcaagggcc
     2761 gtcgctctct ggcgaattac atatcttctg ctcggtgacc gctgcctaca gccatctgcc
     2821 gccgattctg gatcgcttcc gcgcggaaca cccgtcggtg gagatcaaac tcaccactgg
     2881 tgatgcggca gatgcaatgg aaaaagtggt cacgggagaa gcggatctgg cgattgccgg
     2941 gaaaccggag acgctacccg gcgctgtggc gttttcgatg ctggagaatc tggcagtagt
     3001 gctgattgcc cccgcgctgc cctgcccggt gcgtaatcag gtatctgtcg aaaagcccga
     3061 ctggtcaacg gtgccgttta ttatggccga tcaggggccg gtacgccgcc gcattgaact
     3121 gtggtttcga cgcaataaaa tcagcaaccc gatgatttac gccacggttg gcgggcatga
     3181 agcgatggta tcgatggtgg cgctcggctg tggtgtggcg ctgttgccgg aagtggtgct
     3241 ggaaaacagc ccggagcctg tacgcaaccg ggtaatgatt ttagatcgca gcgatgagaa
     3301 aacgccgttt gaactgggcg tgtgtgcaca aaaaaagcgg ctgcatgagc cgctgattga
     3361 ggcgttctgg aggattttgc cgaaccacaa atgacgttgt cgcgcgggta ggcctgataa
     3421 gcgaagcgct atcaggcatt tttccctaac ccgccaaaaa gaacctgaac gccggattat
     3481 tggtttcgtc gtggcaatcg tagcccagct catttagccg ggtttcgaaa tccggttcat
     3541 ggtcgccgag ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt
     3601 ggaacaaaga aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg
     3661 gtgattccgg gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc
     3721 cgaccatata gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt
     3781 agccgccgtc gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc
     3841 gcacgccgac aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga
     3901 ccgatcgccc gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg
     3961 tcaccgccaa caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca
     4021 ggccgtggaa gttcacgttg gcaccggaaa gaatatgcgc caggcgttcg ccgcgaatgt
     4081 tgtgctgagc gatatatttt ttcattcccg ccagcgccaa cgcgccagag ggttccgcca
     4141 ctgcgcgcac atcttcgaat aaatccttca tcgccgcaca gatcgcatca ctatcgacgg
     4201 tgatgatgtc gtcgagatac tcctggcata aacggaaggt ttcgtcgccg atgcgtttta
     4261 ccgcgacacc ttcggcgaac agtcccacgc gcggcagatc aaccggatga cccgcatcca
     4321 gcgctgcttt caggcaggcg gagtcttccg cttctacggc gatcactttg atttgtggca
     4381 tcagttgttt gatcagcacc gcaacgcccg ccgccagacc gccgccgccg actggcacaa
     4441 atacgcggtc gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg
     4501 caatcaccat cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt
     4561 cgatcgcttt agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga
     4621 atccacgtac cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct
     4681 tcacgcctaa ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag
     4741 tgatcacgcc gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc
     4801 gcagcttaaa gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa
     4861 gacgcgacga cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg
     4921 gcgcgcgcag cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg
     4981 agtcagccat tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct
     5041 ggttgccagg ctggcgtagg cgcgcagggc aaaggaaacc tgacgttcac ggtttttcgg
     5101 cgtccaggct ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc
     5161 gcttacctgt aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc
     5221 aatcagacca atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc
     5281 agaggtgccg ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat
     5341 tgatttcagg aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc
     5401 ttcatagcgg atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac
     5461 cgcgtcgtcc tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc
     5521 gacgcccgct gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag
     5581 gccgccgtct ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc
     5641 atcgagagaa tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg
     5701 gcctgcgcgg aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc
     5761 cagagtttgc ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc
     5821 gcgatccagt tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg
     5881 gtatttttgg gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc
     5941 gatatcactc atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac
     6001 cgtgttggta gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt
     6061 gctagcaata ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa
     6121 ttcaacaatg cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag
     6181 cagcgagccg ttgcctggct gcgacaaacc cagcgcttcg gtcaggcagt tcattgagtt
     6241 agcggtaaac atcccggagc aggaaccgca ggttgggcag gcggaacgtt caacctgatc
     6301 gctctgggag tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag
     6361 cttgatgatc tgctcggaca gtttggtttt cccggcttcc atcgggccgc cggaaacaaa
     6421 gatcaccgga atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca
     6481 gttagagatg cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc
     6541 agcgatcagt tcgcgagatg gcagtgaata aagcatcccc ccgtggccca tagcaatccc
     6601 atcatccacc gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc
     6661 ggcgaccagt ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga
     6721 gttcacaacc gcgataatcg gcttgccaaa atcggcatcg gtcattccgg tggcgcgcca
     6781 cagcgcacga gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg
     6841 catactttat ttactcccag tgtctgtctc gtaaatggga cggtaagtgc cgtcccattt
     6901 tttgtattta ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga
     6961 agaggccgaa gaaggcttgc tgaatgcatt tggtaaccgg gccgcaacgg ccttcaccca
     7021 cctgaatacc gtctacactg cgcactggcg tgatttctgc cgccgtaccg gacataaaca
     7081 cttcatccgc caggtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca
     7141 gctctttcgc cagtttgatg atggcatcac gggtaatacc cggcagcgcg gatgaggtaa
     7201 acggtggggt gaacagcacg ccgtctttca cttcaaacag gttttcacct gcgccttcag
     7261 agatgtaacc gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc
     7321 tacccaccag cagggaagag aggtagttac caccggcttt tgccgccgtc gggatggtgt
     7381 ttggtgctgc gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg
     7441 cgcccagata cgctccccac gggaaagcag cgataatcac gtcggttgag tatcccgctg
     7501 gcgggtttac tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg
     7561 tgagattatt tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgcg
     7621 aaaccgggaa gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc
     7681 ggaatacaac cggtcctttg tgcgagtcgt agcaacggat gccttcaaaa accgaagtgc
     7741 catagtgcag cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc
     7801 cattgaacca aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag
     7861 gcgcggattt gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc
     7921 agtttattta actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg
     7981 atatttatat tttgtgcatc gctggcggcg gccatattca ttgagcagac gtggaaacca
     8041 cgatgacgca ccacgcgtaa aacacgttct aaggtttctg gattgaagcg agccgataca
     8101 ttgacctgat gttgcatcat gataatttct ccaacatttc tgaattactg gcgccaggcg
     8161 gcaccagcgg ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat
     8221 cactgttcag catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc
     8281 cagggatgcc gaaggcgctg gctaacatga ggaaatcggg gttatcagta agggtggttt
     8341 cgctgtatcg ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt
     8401 tatcgagtaa gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca
     8461 cattcatcat gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga
     8521 cttgtgcgcc aaccgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga
     8581 tgaaattttc cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca
     8641 catctgtggt cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg
     8701 gcgcgtagat agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg
     8761 cgcagtattg ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat
     8821 ttaaatcacc ctgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga
     8881 tatccatatg gataacactg gcgtgtggtg cgaaagtatt cagtttgccg gtcacccggt
     8941 catcaaaacg tgcgcccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg
     9001 ctttgatgcc gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc
     9061 ccagcccttt cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag
     9121 ccggaactgc ctgcgccata cccacgccac cgccaacgta cagcatcggt ttttgcgctt
     9181 ttgccagcat ctggcgcgct tgctcaactt cggcatgtgg gaaagtcact tcgttttcaa
     9241 cggtggtgaa ccacggttcc aggtcaccgc tggctaactg gatatctttt gggatatcga
     9301 ccagaaccgg accaggacga cctgagcagg caacgtcgaa tgcttcagcc atgatgcgcg
     9361 gcaactcttc cagcgactgc accagaaagc tgtgcttggt acaggctaac gacaatccta
     9421 ggacatccac ttcctgaaat gcgtcagtgc cgataaacgg tgcggacact tgaccggtga
     9481 tggcaacaac agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg
     9541 cgcccggacc agacgtggcg atacatacgc cagttttgcc ggtagcacgg gcataaccga
     9601 tagccgccat tgccgcaccc tgctcatgtc ggcatagcaa gtgctccacg ccgccgtcat
     9661 acaatgcatc gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac
     9721 cctgtgcccg caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct
     9781 gaatcttgag aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt
     9841 aaggtcaaaa aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat
     9901 ctctaagcct ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat caccaccacg
     9961 ctaatcacga ctaggctaat cactcgtaga agggctgtca ttttgtcttt tcttgcatct
    10021 tgttcgaagg aatgcctaaa gagttaccat agattttgcg aatagcacaa gatatttttg
    10081 ggcctatttt tatgatagct ttaataattt caatagaagt tgttgttttt taaaaagaaa
    10141 atagtgagtg aaaaaaattc aatttcagta taaatgttgt tcccttcctc gtagttcatc
    10201 ctttgtccgc tgcatttttg cgagcatcat tccaccgcca gaacaactca acttcacttg
    10261 cacaaataaa caggaatcag ccactaaaat tcacgaatta gcgggctaca gcagcgatat
    10321 cttatgcaca taatggtgac tcaaggaggg cttatgtcac tgtcaattgt tcatacccgc
    10381 gcagccctgg gagtaaatgc gccccctgat cactgttgag gtacatatca gtaaaggtct
    10441 acccggctta acgatggtgg gcttaccaga aacaactgta aaagaagctc gcgatcgcgt
    10501 gcgcagcgcc attatcaata gcggatatga atatccggcg aaaaaaatca ccatcaacct
    10561 tgctccagcc gatctgccaa aagaaggggg acgatatgat ttacctatcg ccattgcgtt
    10621 gctggcggcc tcagaacagc ttacagccaa taagttagat gaatatgaat tagtcggaga
    10681 actggctctt acaggcgctc tgcgtggcgt tcccggtgca atctccagtg caactgaagc
    10741 tattaagtcg ggcagaaaaa ttatcgtcgc gaaagataac gaagatgaag tggggctaat
    10801 taacggtgaa ggatgcctga tagccgatca tctgcaagct gtctgtgcgt ttctggaagg
    10861 taagcacgct ctcgaacgcc cgaaaccaac tgatgcagta tcccgggcgc tacaacatga
    10921 tctcagtgat gttgtcggtc aggaacaagg aaagcgagga ctggaaatta ccgccgctgg
    10981 caggcacaac cttttactga ttgggccgcc gggaacaggt aaaacaatgc tcgccagccg
    11041 tattaatggc ctgttgccag atttaagcaa tgaagaggca ctggagagcg ctgcgatatt
    11101 aagtctggta aatgctgaat cagtacaaaa acaatggcgg cagcgcccgt tccgctcacc
    11161 tcatcacagt gcgtcgttaa ctgcgatggt gggcggtggt gcaattccag ggccaggtga
    11221 aatttcgttg gcg
//
LOCUS       DAJVRF010000127        11141 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJVRF010000127 DAJVRF010000000
VERSION     DAJVRF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11141
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.127"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            60..986
                     /locus_tag="OQN13_003148"
     CDS             60..986
                     /locus_tag="OQN13_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1616432.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFERAEQTPGIKADWLV
                     RMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQARTLPDMLSSDAG
                     PLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(936..2129)
                     /locus_tag="OQN13_003149"
     CDS             complement(936..2129)
                     /locus_tag="OQN13_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1616433.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2265..3989
                     /gene="iucA"
                     /locus_tag="OQN13_003150"
     CDS             2265..3989
                     /gene="iucA"
                     /locus_tag="OQN13_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCS1616434.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3990..4937
                     /gene="iucB"
                     /locus_tag="OQN13_003151"
     CDS             3990..4937
                     /gene="iucB"
                     /locus_tag="OQN13_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCS1616435.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4937..6679
                     /gene="iucC"
                     /locus_tag="OQN13_003152"
     CDS             4937..6679
                     /gene="iucC"
                     /locus_tag="OQN13_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCS1616436.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6676..8013
                     /gene="iucD"
                     /locus_tag="OQN13_003153"
     CDS             6676..8013
                     /gene="iucD"
                     /locus_tag="OQN13_003153"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCS1616437.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8019..10214
                     /gene="iutA"
                     /locus_tag="OQN13_003154"
     CDS             8019..10214
                     /gene="iutA"
                     /locus_tag="OQN13_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCS1616438.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDFLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10765..10908)
                     /locus_tag="OQN13_003155"
     CDS             complement(10765..10908)
                     /locus_tag="OQN13_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616439.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2799 a         2789 c         2882 g         2671 t
ORIGIN      
        1 acccacaagt tgaaataata gtattatatt gaaaagacag agattatttc agaggtgtta
       61 tggaaaactg ggttattcag gagctaaaat cactggatat aggggactca agacttgata
      121 aaagagtaaa acatatcctc aatagcctca gtcgttcacc agaagaaagt attcctgttt
      181 cctgccgtac ctggagtgaa acaaaggcag cttatcgttg tttttcaaac gataaagtct
      241 ctgctaataa aattatggct cctcacaaag aaaatatcac tgaaagagct cagcagttta
      301 aaagagtttt agttttacag gatacaacag aacttaatta ttctggtcag aaagaaaaac
      361 aaggagttgg tcacaaaaaa cataaagatg aaagaaactt atttcttcat cctcaactcg
      421 tcatcagtga gtcaggtgtt tgcctcggtg tttatgatga ctatcagtgg ttcagggatg
      481 aattaaaaac cagtaaaaat acccgacaag aaatctgcaa tgatttactt cataaaaaac
      541 atgtcagtga aaaagaaacc tggcgttggg ttgaaggata taataaagca acagaactgg
      601 ctcgccaatg ccctgatacc catgtattgt caatttctga tcgtgaaggc gatttttatg
      661 acctgtttga acgcgcagag cagactcccg gaataaaagc tgactggctt gtcagaatga
      721 aatttaataa cagagcaacg ctgaatatta atggtaaacg ggatcatagg ttgttgcacg
      781 aaagaatcat ggagattaca ccgcagcaac tggttgaatt cactatacct gatgggcggg
      841 ggcagcaagc cagaacactg cctgacatgc tttcatcaga cgcaggtcct ctctcatatc
      901 ctctgattta ctccagtaag gtgatgatca tatctttatt tgatagtacg gtaatgccag
      961 ataaatacag acacaatggt aaataatata gttgcaaata atgctgccat aatcagcgcg
     1021 attgcgacac ctgttttcga taccagcggc accagcaaag cgcacagtcc atagccaagt
     1081 gtgtgactgg tagcgagcca gcccgcgcct gcgccatcag ccagcttatc attcagtaga
     1141 agttgataag cgggtgttgc cagagctgct ccaaatgaca gcactgcaca gcccgggtaa
     1201 aacaaccata gctgttcgga gagcatgata gccagaccac cactcatcaa cactccggca
     1261 ctcaggagca gggccaccgg agtcaggcgc tgcggacgga gtaccccgaa ctgcgcgata
     1321 agcgcagcta ctgcggacag acccaacagc caggccacct gttggctaat ggcggtggta
     1381 tccgtgacga actggcgagt aagggcaggc gaaagtccaa gctgcatcat gctcaccgcc
     1441 gcagccagta atattgcgca aagcagataa ggcagacaat cccgcttcag actgacgctc
     1501 ttgcactccg gtgtgggctg tggtggtgtg ccgggcaacc gcagcagcat cagcagcgcc
     1561 agcactggtg ctgccatcag tagccccagt ggcgccagag gatgaatggc cagcattgct
     1621 gccgcgcaca gcggcccgaa tagcctgccg caactcaggc cggagctgat ggttgccaga
     1681 gcggccatgc gattcccttc tccagctctc tgcaatgccc agacctgaca ggctggcacc
     1741 attgccgaca cggtcagccc gtaggcgatc cgggcgatga ttaatattcc cagtcccacc
     1801 gctgttgtta tggcgctggt agccatcagc acgcttccca gccctagcag ggtaaagctt
     1861 atgccatatc ctcccagagc ccacaacacc acaggtttac tgccagtacg ggaaatttgc
     1921 tttccccacc acggggacga tggcagaaac agcattgagc caagcatcag cagggcagcc
     1981 catacagaca gactcagatt tgtctgtatg accagaacag ggagcacaac cagcagaccg
     2041 ttctgtccga taccgagaag cccggcactg aacgcaagtg gccagcagga cagtgttttt
     2101 tggggcgtat cttcgatccc tggtgacatt ataatgtttc aactccatgt attaattgtg
     2161 tttatttgta aaattaattt atctgacaat aacatttatt gttgataatg agaatcatta
     2221 tcgacataat tgttattatt ttactgtgtg ggagctgttt gactatgacc ctgccctctg
     2281 aaaaaccagc cacagatgtg gctgcgcagt gcttcctgaa tgcactgatt cgtgaaacca
     2341 cagactggaa actgacagaa tacccgccag acgaattgct catcccgctg gatgagcaga
     2401 aatcgctcca tttcagagtg gcttatttct ccccaaccca gcatcaccgc tttgcatttc
     2461 ctgcacgtct ggtcacggca tcaggcagtt atcctgtcga ctttaccact ctctcccggc
     2521 ttattattga taagctacgc catcaactgt ttctgcccgt tcccctctgc gaaactttcc
     2581 accagcgcgt gctggaaagc catgtccata cgcaacaggc aattgatgcc cgtcatgact
     2641 gggccgctct gcgtgaaaaa gcgttgaatt ttggcgaggc tgagcaggcg ctgctgacag
     2701 gacacgcttt ccaccctgcg cctaagtctc atgaaccgtt taaccgacgg gaggctgaac
     2761 gctacctgcc tgatatggca ccccacttcc cactgcggtg gttttcggtg gataaaacgc
     2821 aaatcgccgg tgaaagtttg catctcaacc ttcaacagcg gctgacgcga tttgccgcag
     2881 agaatgcgcc tcagttactc aacgaattaa gtgacaacca atggctgttc ccgttgcacc
     2941 cgtggcaggg ggaatatctt ttgcagcagg ggtggtgcca ggcacttgtt gctaaagggc
     3001 tgattaaaga cttaggtgag gccggcacgt cgtggctgcc gaccacctct tcccgttccc
     3061 tctactgtgc caccagccgc gatatgatca agttctccct gagcgtacgg ctgaccaact
     3121 ccatccgtac tctgtccgtg aaagaagtga agcgtgggat gcgcctggca cgcctggctc
     3181 aaaccgacgg ctggcagatg ctacaggtcc gcttcccgac tttccgggta atgcaggagg
     3241 atggctgggc cgggctgctc gatcttaacg gcaacatcat gcaggaaagt ctgtttgccc
     3301 tgcgtgaaaa tctgctggtg gatcagccga aaagccagac taacgtactg gtctccctga
     3361 ctcaggccgc acctgatggc ggtgattcgc tgctggtttc ggcggtaaaa cgcctgagcg
     3421 atcgcctcgg tatcactgtg caacaggccg cccatgcatg ggtcgatgca tactgtcagc
     3481 aagtgctaaa gccgctgttt acggctgaag cggattacgg cctggtgctg ctggcgcatc
     3541 agcaaaatat tcttgtccag atgcttgggg atctgccggt cggatttatt taccgtgact
     3601 gtcagggcag cgcttttatg cctcacgcga cagactggct cgattctatt ggcgaggcgc
     3661 aggcggaaaa tattttcacc catgagcagt tgctgcgcta tttcccttat tacctgctgg
     3721 ttaactccac ttttgctgtg accgctgcgc tgggggctgc cgggctggac agcgaatcga
     3781 atctgatggc tcgtgtacga gcatcgctgg ctgaagtgcg tgatcaggtg actcataaaa
     3841 cctgtctcaa ctacgtgctg gaaagtccgt actggaacgt aaaaggtaac tttttctgtt
     3901 atctgaacga tcataacgag aacaccattg ttgacccttc ggtgatctat ttcgatttcg
     3961 ctaacccgct gcaggctcag gaggtctgaa tgtctgaggc aaacattatt cacagcagat
     4021 atggactgcg ctgtgaaaaa ctcgacaagc ccctgaatct tggctgggga ctggacaata
     4081 gcgcggtgtt gcactgtccc ggggagctgc cgacagggtg gctgtgcgat gcgctggatc
     4141 agatatttat cgccgcacca caactgtcag cagtggctct cccctgggcc gaatggcgtg
     4201 aggagccaca ggcgctgacg ctttttgggc aggtaaaaag cgacattatc catcgtactg
     4261 ccttctggca gttaccgtta tggttgagtt caccggcaaa ccgggcctcc ggtgaaatgg
     4321 tttttgatgc agagcgtgag atttatttcc cgcagcgccc accccgtcca cagggtgagg
     4381 tttaccgccg ttacgatcca cgtattcgca ggatgctgag tttccgcatt gccgatcccg
     4441 tttctgatgc agaacgtttc acccgctgga tgaatgatcc gcgcgttgag tatttctggg
     4501 agcaaagtgg ctcactggag gtacagaccg cctatctgga gcgccagctc accggtaaac
     4561 atgcattccc gctgattggg tgcttcgacg atcggccatt cagctatttc gaaatctact
     4621 gggcggcgga agaccgcatt ggtcgtcact attcgtggca gccctttgac cgtggcctgc
     4681 atctgctggt tggtgaacag caatggcgcg gggcccacta tgtgcaaagc tggctgcgcg
     4741 ggctgacaca ttacctgctg ctggatgagc cccgtacgca gcgcacagta ctggagccac
     4801 gcaccgataa ccagcgcctg ttccgccatc ttgagcctgc gggataccgg acaattaaag
     4861 agttcgactt cccacataag cgctcgcgca tggtgatggc ggatcgtcat cacttcttca
     4921 cggaggtcgg tctgtaatga atcacaagga ctgggatttt gttaaccgtc ggctggtggc
     4981 aaaaatgttg tctgagatgg agtatgagca ggttttccac gcggaatctc agggcgatga
     5041 ccactactgc attaacctgc cgggagcaca atggcgcttc atcgctgaac gtggtatctg
     5101 gggctggctg tggattgatg cccaaacgtt gcgctgcacg gacgaaccag tactggcgca
     5161 gacgctgctg atgcagctaa agccggtgct gtcaatgagt gatgccactg ttgcagagca
     5221 tatgcaggat ttgtatgcca cgctactggg cgacctgcaa ctgctgaagg cccgtcgcgg
     5281 actgagtgcc agtgacctga ttaatcttga tgccgatcgt ctgcaatgcc tgctgagcgg
     5341 tcatcctaaa ttcgttttta ataaaggtcg ccgtggctgg ggcaaagagg cgctggaacg
     5401 atatgcgcca gagtatacca acaccttcag actgcactgg ctggcggtaa aacgtgaaca
     5461 tatgatctgg cgctgcgata acgatctgga tattcagcag ttgttgactg ccgcaatgga
     5521 tccgcaggag tttacacgct tcagtcaggt ctggcaggaa aacgggctgg atcacaactg
     5581 gctaccgctg ccggtacatc cgtggcagtg gcagcaaaaa atcgccacag acttcatcgc
     5641 tgattttgtc gaaggcagga tggtgtctct cggcgagttt ggcgaccagt ggctcgccca
     5701 gcagtcgctg cgtaccctga ccaacgccag ccgccgggga gggctggata tcaagctgcc
     5761 gctgaccatc tacaacacct catgctaccg gggaatccct ggcagatata tcgctgccgg
     5821 accactggct tcacgctggc tgcaacaggt ttttgcgacc gacgccaccc tggtgcaaag
     5881 cggcgcagtg atccttggtg aaccggctgc aggctatgtg tcccatgaag gctatgccgc
     5941 gcttgcccgg gctccctatc gctaccagga aatgcttggt gttatctggc gggagaatcc
     6001 gtgccgctgg ctgaaaccgg atgaaagtcc tgttctgatg gcaacactga tggagtgcga
     6061 cgaaaacaat cagccgctgg caggcgcata tatcgaccgc tccggactgg acgctgaaac
     6121 ctggcttacg caattgttcc gggtggttgt ggttccgctg tatcacctgc tttgccgcta
     6181 cggtgtcgcg cttattgcac acggacaaaa tataacgctc gccatgaaag agggggtacc
     6241 acagcgtgtt ctgctgaaag acttccaggg cgatatgcgg ctggtgaaag aagcgtttcc
     6301 cgaaatggac tctttgcctc aggaggtgcg tgatgccaca tcccgtctga gtgcggacta
     6361 cttaatccat gatttgcaga cgggtcactt cgtgacagta ctgcgtttta tttcgccact
     6421 gatggttcgt cttggcgtac ctgaaaggcg attttatcaa ctgctggcag cagtgttgag
     6481 tgattacatg aacaaacatc cacaaatggc agagcgtttt gcgcttttct cactcttcag
     6541 gccacaaatc attcgcgtgg tgctgaaccc ggtaaaactg acctggccgg acctggatgg
     6601 cggcagtcgc atgctgccga attaccttga gaatctgcaa aatccgctgt ggctggtaac
     6661 tcaggaatat gaatcatgaa aaaaagtgtc gattttattg gtgtcgggac agggccattt
     6721 aatctcagca ttgctgcatt gtcacatcag atcgaagaac tgaactgtct cttctttgac
     6781 gaacatcctc atttttcctg gcatccgggt atgctggtac cggattgtca tatgcagacc
     6841 gtctttctga aagatctggt cagtgcagtt gcacctacaa atccctacag ctttgttaac
     6901 tatctggtga agcacaaaaa gttctatcgc ttccttacaa gcaggctacg tacagtatcc
     6961 cgtgaagagt tttctgacta tctccgctgg gctgctgaag atatgaataa cctgtatttc
     7021 agtcataccg ttgaaaatat tgatttcgac aaaaaaagtc gattgtttct ggttcagacc
     7081 agtcggggag aatattttgc ccgcaatatc tgccttggta caggaaaaca accttattta
     7141 ccaccctgtg tgaagcatgt gacacaatcc tgtttccatg ccagtgaaat gaatcttcgt
     7201 cggccggacc tgagtggaaa acggataacc gtggttggtg gaggacagag tggtgcagac
     7261 ctgttcctta atgcattacg cggggaatgg ggagaagcgg cggaaataaa ctgggtctca
     7321 cggcgtaata attttaacgc actggatgag gctgcttttg ctgatgagta ttttacacct
     7381 gaatatattt caggcttctc cggactgaag gaagatattc gccatcagtt actggatgag
     7441 cagaaaatga catcggatgg catcactgcc gattctttac tgaccattta tcgtgagttg
     7501 taccaccgtt ttgaagttct gagaaaacca agaaatatcc gtctgctacc cagtcgctcg
     7561 gtaacaaccc tggaaagtag tggtccgggc tggaggttat tgatggaaca tcatctggat
     7621 cagggcagag agagcctgga aagtgatgtg gtgattttcg ccacaggtta ccgttctgca
     7681 ttgccacaaa tacttccctc actgatgccc ttgatcacca tgcacgataa gaacaccttt
     7741 aaagtgcgtg atgacttcac tctggaatgg agtggcccga aagaaaataa catctttgcg
     7801 gtcaacgcca gcatgcaaac tcatggcatc gccgaacccc agctcagcct gatggcctgg
     7861 cgatctgcac gtattcttaa tcgcgtactg gggcgtgatt tattcgatct cagtatgccg
     7921 cccgcactga ttcagtggcg cagcggcagc cggaaaaaac cgcagccgga ggctgcttct
     7981 ttaactcact atacagcaaa tattcaggaa taacgatgat gcgcaaaaag tatatgcccc
     8041 gggctcttgg tccgctgctt cttgtcgtgc tgtcaccagc tgtcgcccag caaaacgatg
     8101 ataatgagat catagtgtct gccagccgca gcaatcgaac tgtagcggag atggcgcaaa
     8161 ccacctgggt tatcgaaaat gccgaactgg agcagcagat tcagggcggt aaagagctga
     8221 aagacgcact ggctcagtta atccccggcc ttgatgtcag cagccagagt cgaaccaact
     8281 acggtatgaa catgcgtggc cgcccgctgg ttgtcctgat tgacggtgtg cgcctcaact
     8341 cttcacgttc cgacagccga caactggact ctgtcgatcc ttttaatatc gaccatattg
     8401 aagtgatctc cggcgcgacg gccctgtacg gtggcgggag taccggaggg ttgatcaaca
     8461 tcgtgaccaa aaaaggtcag ccggaaacca tgatggagtt tgaggctggc acaaaaagtg
     8521 gctttaacag cagtaaagat cacgatgagc gcattgccgg tgctgtctcc ggcggaaatg
     8581 accatatctc cggacgtctt tccgtggcat atcagaaatt tggcggctgg tttgacggta
     8641 acggcgatgc caccctgctt gataacaccc agaccggcct gcagcactcc aatcggctgg
     8701 acatcatggg aaccggtacg ctgaacatcg atgaatcccg gcagcttcaa ctgataacgc
     8761 agtactataa aagtcaggga gacgacaatt acgggcttaa tctcgggaaa ggcttttccg
     8821 ccatcagcgg gagcagcaca ccatacgtca gtaaggggct gaattctgac cgcattcccg
     8881 gcactgagcg gcatttgatc agcctgcagt actctgacag tgatttcctg ggacaggaac
     8941 tggtcggtca ggtttactac cgcgatgagt cgttgcggta ctacccgttc ccgacggtaa
     9001 atgcgaataa acaggcgaca gctttctcct cgtcacagca ggataccgac cagtacggca
     9061 tgaaactgac tctgaacagc caactgatgg acggctggca aatcacctgg gggctggatg
     9121 ctgagcatga gcgctttacc tccaaccaga tgttcttcga tctggctcag gcaagtgctt
     9181 ccggagggct gaacaaccat aagatttaca ccaccgggcg ctatccgtca tatgacatca
     9241 ccaatctggc ggccttcctg caatccagct atgacattaa tgatattttt accgttagcg
     9301 gtggcgtacg ctatcagtat actgagaaca gggtagatga tttcatcgac tacacgcagc
     9361 aacagaagat tgctgccggg aaggcgatat ctgccgacgc cattcctggt ggttcggtag
     9421 attacgataa ctttctgttc aatgctggtc tgctgatgca catcaccgaa cgtcagcagg
     9481 catggttcaa tttttcccag ggggtggcat tgccggatcc ggggaaatat tatggtcgcg
     9541 gcatctatgg tgcagcagtg aacggccatc tttccctgac aaagagcgtg aacgtcagcg
     9601 acagtaagct ggaaggcgtg aaagtcgatt cttatgaact gggctggcgc tttaccggtg
     9661 acaacctgcg gactcaaatc gcggcatatt actcgctttc caataagagc gtggaaagga
     9721 ataaagatct gaccatcagt gtgaaggacg acaggcgccg tatttacggc gtggaaggtg
     9781 cggtggactt cctgatcccg gatactgact ggagtaccgg tgtgaacttc aatgtgctga
     9841 aaaccgagtc gaaagtgaac ggtcaatggc aaaaatatga cgtgaaggaa tcaagtccat
     9901 cgaaagcgac agcttacatt aactgggcgc cggaaccgtg gagtctgcgt gtacagagca
     9961 ccacttcttt cgacgtaagc gatgcagagg gtaacgatat taatggttac actaccgtcg
    10021 attttatcag tagttggcag cttccggtgg gaacactcag cttcagcgtt gagaacctct
    10081 tcgaccgtga ctataccact gtctggggac agcgtgcacc tctgtactac tgcccgggtt
    10141 acggccctgc ttcactgtac gactacaaag gccggggccg aacctttggt ctgaactact
    10201 cagtgctgtt ctgacgggga tgcttttgca acaagggtat gctgtaaaca acatggtcct
    10261 tggtggccat gttgtttaca gccagtataa tatacagcct taacaagata ttccgtctcc
    10321 ggaatggact gttcaccact gaataacatt atttctgttt tatggatctt tgactgaaat
    10381 ttatcgcata tcctcatttc attgtccgtc tgaaaactaa ccttacaagt aagcgtcgtc
    10441 tcagcaccgt ctggcagatc ctgaaattcc tgagaggatg atctgcgccc aatatcctga
    10501 gccggaactt tgctgcaatg aaggccaacg aaaaatgggg gagtatgtta tagagtactt
    10561 gctacagggt aaaaagaggt acctgtcgct ggttctccgt tcagtttaac cgggagattt
    10621 tctcctgcgc ctgtcataaa gtccggtaat ggagattgtt aatacgatgg tgcacgatgc
    10681 attgtcggtg cccggcccgg aggatgcccc gtaactacat acggatcagg gttggcaata
    10741 tcgaatggca ggctataggc aaagttaaag ccccatggag tagcacaaaa tatgccacac
    10801 aaaggaaacg gtctgaataa cgcagtgatg gagaacttct tcggcacact gcaaaatcgc
    10861 atcgtggtcg atcgctaatt cgccgtgaat cactgaacga tgttccatgc gaacagatat
    10921 tcgatcatat cgaaatatta aacattaccc ctccccgcat tgggcaaaaa aaaaacaaaa
    10981 aacgatcaat tagtattgat tcacataatc tctcatcatt aatgagaaca ttgtcacata
    11041 tttacaatat agaactgtta cttttagtaa gctgttagta accaactgtt actaataatt
    11101 ttaattctta atactttgtt cattcaataa atgagttgag a
//
LOCUS       DAJVRF010000128        10930 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJVRF010000128 DAJVRF010000000
VERSION     DAJVRF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.128"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..312)
                     /locus_tag="OQN13_003156"
     CDS             complement(<1..312)
                     /locus_tag="OQN13_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCS1616440.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="OQN13_003157"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="OQN13_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCS1616441.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="OQN13_003158"
     CDS             complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="OQN13_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCS1616442.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="OQN13_003159"
     CDS             complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="OQN13_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCS1616443.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="OQN13_003160"
     CDS             complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="OQN13_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCS1616444.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="OQN13_003161"
     CDS             complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="OQN13_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCS1616445.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSVPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="OQN13_003162"
     CDS             complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="OQN13_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCS1616446.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="OQN13_003163"
     CDS             complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="OQN13_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCS1616447.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDSPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTTFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8292..9365)
                     /locus_tag="OQN13_003164"
     CDS             complement(8292..9365)
                     /locus_tag="OQN13_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1616448.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(9376..>10930)
                     /locus_tag="OQN13_003165"
     CDS             complement(9376..>10930)
                     /locus_tag="OQN13_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1616449.1"
                     /translation="VTIKEEDGSEQRFTQPYASLAILKREGQTDVDVSVGELRDEDGF
                     TPDVLQAQILHGFSHGITLYGGMQAAENYGSAALGVGKDLGALGAISFDVTHARANFS
                     HDDTETGQSYRFLYSKRFDDTDTSLRLVGYRYSTEGYYTLNEWASRRNSPEDFWETGN
                     RRSCVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSY
                     SNTARQGTGNNHASDNTSEQIYMLSLSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLS
                     GTALERNNLSWNLMQSYNSHDDEVGGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRG
                     GILAHSEGVTLSQELGETIALVKAPGAAGLEIDNMRGAATDWRGYTVKTQLNPYDENR
                     VAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGA
                     IAAIQDASLADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSLSTPESESG
                     LIEQGVTCH"
BASE COUNT         2851 a         2830 c         2480 g         2769 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccaaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc caaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag
     2281 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     2341 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     2401 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     2461 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     2521 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     2581 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     2641 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     2701 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     2761 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     2821 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt
     2881 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
     2941 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
     3001 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
     3061 tttcgccagc gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt
     3121 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
     3181 caaggacctt ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag
     3241 gttttgcctg cgaatgcagt aacaatccag tcatttatta atgtttttta taacgaacat
     3301 ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgcgct attgataaaa
     3361 atatgaccat gctcgcagtt attaactttg tgtaatttta ggaatttata aagttatata
     3421 taacaaatcc caataattaa gttattggga tttgtctggt gaattatttg tcgctatctt
     3481 ttcccgccag cagtttatcc agctcatcac cgcctacatg acggaaatcc tgccccttca
     3541 cgtagtagaa gataaactca caaatattct ggcagcggtc gccaatacgt tcgatagaac
     3601 gcgcgcagaa cagcgcagta agtacgctcg gaatggtacg cgaatcttcc atcatgtagg
     3661 tcatcagttg acgaacaata ccttcgtatt cctgatcgac ttttttatct tcacgataaa
     3721 tacgtaccgc ttcgtcaatg tccatccgcg cgaacgcgtc cagcacgtcg tgcagcatct
     3781 ggatggtatg acggcccagc gactccagac ttaccagcaa cggctgatgc tgctgggaga
     3841 atttctccag cgcagtacgg cagattttgt ccgccacgtc gccaatacgc tccagctcgg
     3901 caatggtttt actgatcacc ataaccagtc gcaggtcgct cgccgtcggc tgacgtttgg
     3961 cgataatgcg cacgcaggct tcatcgatcg ccacttccat catgttgacg ttcttgtcgc
     4021 cttcgatcac gcgcttcgcc agatcgctgt cctggttatg catcgcggtg attgcatcag
     4081 aaagctgctg ctccaccatg ccacccatgg tcatcacctg cgtgcggata ctttccagtt
     4141 cggcgttgaa ctggccggaa atatgtttat taagattgag actgtccata acgcactcct
     4201 gaattaaccg taacgaccgg tgatgtagtc ttctgtttgt ttcttcgctg gcttggtgaa
     4261 cagatcgtcc gtgttgctga actcaatcaa ttcgcccagg tacataaacg ccgtgtggtc
     4321 ggaacaacgc gcagcctgct gcatgttgtg ggtaacgatc accacggtgt aatcctgctt
     4381 cagttcggtg atcagctctt caatacgccc ggtagagatg gggtcgagcg ccgaacacgg
     4441 ttcgtcgagc agcagcactt ccgggcgaat ggcgatacca cgcgcaatac acagacgctg
     4501 ttgctgacca ccagagagag agtaaccgct ctggtgcaat ttatctttgg tttcgttcca
     4561 caatgcggct ttggtcaatg cccactgcac gcgctcgtcc atgtcggcac gggagagctt
     4621 ctcaaacaga cgaacaccaa aagcgatgtt gtcgtagatg gacatcggga acggcgtcgg
     4681 tttctggaac accatgccca ctttcgcacg cagcagtgcg atatcctgag agttggtcag
     4741 gatgttgtcg ccatcaagca gaatttcacc ttctgcgcgc tgctccggat acagttcaaa
     4801 cattttgttg aaggtacgca gcagcgtcga tttaccgcag ccggacggcc cgataaacgc
     4861 cgttacctgg tttttagcga tatccaggtt gatgtttttc agggcatgga atttgccgta
     4921 gtagaagttc aaattacgaa cctgaatttt actcggggca gtttcaacca tactcattgc
     4981 aatcttttcc tcatcgtacc tgatgctttt cgctaatcag gcttaccgta ggccgggtaa
     5041 ggcgttcacg ccgcatccgg caaagtgtta accgtgttta ttcttcgcaa aaacaacgcg
     5101 cgccagaatg ttcagcagca ggacgcacag ggtaatgatc aataccccgg cccaggccag
     5161 ttgttgccat tccgcaaacg ggctcatcgc aaacttaaag atcgtcaccg gcaggttggc
     5221 gatcggctgc atcatgtccg tgctccagaa ctggttggag agcgcggtaa acagcagcgg
     5281 cgcggtttca ccggcaatac gggcaatcgc cagcaggata ccggtcataa tcccggacac
     5341 cgacgctttc agcgtaatcg cggagatcat cttccacttc ggtgtaccca gcgcataagc
     5401 cgcttcacgc aggctgtccg gcaccagttt cagcatgttc tcggtggtgc ggataacgat
     5461 cggcacctgc aacagcgcca gcgcaattac gcccgcccag ccggagaagt gctccatctg
     5521 tgccaccacg atggtgtaga caaacagacc aaccacaatc gacggcacgg agagcagaat
     5581 gtcgttaatg aagcgaatca cttctgccag ccaggattta cgaccatatt ccgccagata
     5641 aatccccgcc ataatgccca gcggcgtacc gaataccgtg gcccacaaaa ttaacagccc
     5701 gctacccgcc agggcgttcg ccagaccacc accttccgta ttgggcggcg gcgtcatttc
     5761 agtgaacagc gccagcgaca taccgtcgat accgcgagtg atggtggaca ttaaaatcca
     5821 gatcagccag aacaggccga aggccatcgt cgccatcgag agcgtcagcg caatacggtt
     5881 tttgaggcgg cgacgcgcct gcattttgcg gcgagattca gccagcgccg cagtggtttg
     5941 catttcaacc atagccatta gcgtgccccc tcattcttag ccaggcgcat aatcataaac
     6001 ttcgatgcgg cgaggacgat gaaggtaatc acaaacagga tcaggcccag ttccatcagt
     6061 gcggcaacgt gcagaccgga ttccgcttcc gcaaattcgt tcgccaacgc agaggtgata
     6121 ctattgcccg gcatgtacag cgaggcgctg tcgagctggt aggtgttacc gatgataaag
     6181 gtcaccgcca tggtttcacc gagcgcgcgg cccagcccca gcatgatgcc gccgataaca
     6241 ccatttttgg tgaacggaag aacgatacgc cagataactt cccaggtggt gcagccaata
     6301 ccgtaggccg actctttcat catcaccggg gtttgttcga acacatcacg cattaccgcc
     6361 gcaatgtacg gaataatcat gatggcgagg atcacgcctg ccgcgaggat accgatacca
     6421 aatgcggggc cagagaacag cgcgccaaca atcgggatat tcgacatgat attgccgacc
     6481 ggctcctgaa agtaaacggc gaacagcggc gcaaagataa acaggcccca catgccgtaa
     6541 acgatacttg gaatggctgc cagcagctca atggcgatac ccagcgggcg tttcagccag
     6601 ccaggcgcaa gctcagtcag gaacagggcg ataccgaaac tcaccgggac ggcgatcagc
     6661 agcgcgataa acgaagtcac caacgtaccg tagatcggca ccagcgcccc gtagatatcg
     6721 ttcggtgcat cccactcttt ggtccatagg aaagccagac caaatttctg aatgctcggc
     6781 caggaggaga tgatcagaga gacaataatg ccacccaaca tcaatagcac aatcagcgcc
     6841 gccagtttta ccagcacgct gaaaattatg tcgccctttt tacccggtgg gttaaaagca
     6901 ggcttggttg cagccataag ttactcttca gttaaacgcg tttacaaagt cattttgtaa
     6961 tgccggatgc ggcgtaaaac accgtacccg gcctggagtt ttattagtac agcggcttac
     7021 cgctactgtc tttaatattg gtcttccacg cagcgcgaac ctgttcaact acactatccg
     7081 gcaggctggc gtaatccagg tcgttcgcct gtttagcccc ggttttgtac gcccagtcga
     7141 agaatttcag cacttctgtg ccttgttctg gtttcttctg atctttgtgg atcagaatga
     7201 acgtggtaga ggtaataggc catgcatctt cgcctttctg gttggtcaga tcctgagcga
     7261 aggttttgct ccagtctgca ccttttgctg cattagcgaa gttttcttcg gtcggactaa
     7321 ccggtttacc atcagcggag atcagtttgg tgtacgccag gttgttctgc ttcgcgtaag
     7381 catattcaac ataaccaatt gcacccggca gacgctgaac gaacgcggcg ataccgtcgt
     7441 tacctttacc gcccagaccg atcggccatt ttacggtaga gccagtacca acgttgtttt
     7501 tccactcttc gttcactttc gccaggtagc tggtgaagac gaaggtagtc ccggagccat
     7561 ctgcgcggcg tactacagca atgttttgtg aaggcagttt cagacccgga ttcagtttgg
     7621 cgatggtttc atcatcccac ttcttgattt tgcccaggta gatgtcgccg agggttttac
     7681 catccagcac cagttcgcca gacttcagcc ctggaatgtt aaccgccagc accacgccgc
     7741 caatcacggt cgggaactgg aacagacctt cctgagccag tttttcgtca gacagcggcg
     7801 agtcagaggc accaaaatca acggtattag cgataatctg ttttacgcca ccggaagaac
     7861 cgataccctg gtagttaact ttattaccga tttctttctg gtaagtgtca gcccatttgg
     7921 catacaccgg cgcagggaag gttgcacctg cacctgtcag gcttgcttct gcaaacacag
     7981 agaaagcgct catcgataag gtcgcggcga caacagttgc gacggtggta cgcataactt
     8041 tcataatgtc tcctgggagg attcataaag cattgtttgt tggctacgag aagcaaaata
     8101 ggacaaacag gtgacagtta tatgtaagga atatgacatt tttatgacag aaaggaatta
     8161 acaaataact tactgatttt aaatgtgaag atgaaaatcc cacatgtcat ctttttgtca
     8221 tgatgcattt aaaagtgata aagaaatggg tgagatgaca acgatgtgat gaacaatcgt
     8281 tgtcagacct attagcgcac aatctcaaca gttatcgtcg ctgttgcagt aaattgcccc
     8341 ggggctgggc gagctccagt aaggctcgca ggagcagctg aaaaagttac actacctgat
     8401 ttttcaccat acactgttga tttacccatg tctgttggca actcaccacc gttcactttg
     8461 atttccttat tatttttgtc gtaaattttt atccccacgt tagagtttga agttacgacc
     8521 attgaattgt ccgtattggg ttcggtagca taaaaagacg tactgatatc atcggtattc
     8581 agtatcccca ggttttcaca ctcaatagtg actgttttag taattttatg tgttgttacc
     8641 gcacttccaa caacagatga aaaatctgca gctggtattt ctttaaaatc aaaataaact
     8701 ggctgtaaat tatttatttc gcaactttgc ggagctgtaa tatcgccccg aatataaaca
     8761 tcagccatcg gctgtgagta atccaccggg gtgtcacggc taattgttcc atacaatgtc
     8821 actatctttg tttcaggaat aatcaatttt cctgcaaatg tcttcttgat ataaaaatat
     8881 actatcgctt tacccccact ggatatactt aaaggttcaa tgcggttgca attgtacttt
     8941 gtattaccag acgggttgtt agcatgatat tcaaaaggtg cattcacata cccaacccca
     9001 agaatatcta tagcaatccc aacatcaagg ttcggattaa gatcataata attaatatta
     9061 gagtgaaccc cagcataagt taatgcctgg tttatttttg acgtataata gatatagggg
     9121 aattctccta cattaccagt atcacaatga cagcgtccag gatatgagga tccattagtt
     9181 acagtggtat taaatgtcgt tccaacctta ttttcgttcg cggtaataag atatcccgag
     9241 aaatcaagcg tacggtgaaa tacgccatta tcaggggtac agataccttt aggagctgca
     9301 agtgctacac tacttaacga gagcataaac acagcaaagc accactgttt gatatacttg
     9361 ttcataaaaa tccttttaat gacatgtcac accctgttca attaatccgc tctcactttc
     9421 tggtgtcgaa agtgaataat tgaagatgca ttttgttgag gcgttttctc cccaggataa
     9481 cgtaacctgt cctttttctg gtagaccaga aagataaagt tcgccacggt caccgacaat
     9541 tcctgaatct gccaaacttg catcttgtat cgcagcaatg gctccaaaag gtaccggttt
     9601 accatttgca tttaacactc tgaagagaac gcgataaccc acatgagtca caaactccgc
     9661 tttaaccact gcaccacgcg tgggaaccat cgtaacgacg gtattatcaa gttctatatt
     9721 cgacttcgag aaatagttat cgctgattgc tacccgattt tcatcataag ggtttagctg
     9781 tgtcttgacc gtatagccgc gccagtccgt cgcagcaccg cgcatattat ctatttctaa
     9841 cccggcggcc ccaggtgctt taacaagagc aatagtttca cctaactcct gacttaacgt
     9901 taccccttcg ctgtgtgcca gaattccccc tctgatacca taattcagcc tctgggaatt
     9961 ttggctgtag ttatagctgc cgttcaccgt gccataagag ccatcatagg tcagggacat
    10021 attaccgcca acctcatcat catgactgtt ataggactgc attaaattcc atgaaaggtt
    10081 atttctttcc agcgccgtac cgctgagtcc gagttgatgt gagctaccgg aattatcgtt
    10141 ttgcgaaaca gaataggtgg cgtaactatt accccaccag cccaataaag gaacagataa
    10201 agagagcatg tagatctgct cactggtgtt atcactggca tggttgttcc ccgtcccctg
    10261 tctggcagta ttggaataac tccaggagac gttccacgag agacgcttcc agctactgct
    10321 gtagccaaat tgcattaatc gttcgacatc atcggtatgc cagtattgtt gccggcttaa
    10381 tgtcaggtat aaattgccat aatctctccc caacgactgc gttagcgtcc cctccacgca
    10441 actacgtcgg ttacctgttt cccaaaagtc ttcagggctg ttgcgccgcg atgcccactc
    10501 attgagggta tagtagccct cggtggagta acgatagcca accaagcgca agctagtgtc
    10561 tgtgtcgtca aatcgttttg aatagagaaa gcgatatgac tgacccgttt ctgtatcatc
    10621 atggctaaaa ttcgcacgag catgtgtcac atcgaaagaa attgcgccca aagcgccaag
    10681 atctttaccg acacccagag ctgcagaacc ataattttca gcagcctgca tacctccata
    10741 taaagtgatc ccgtgggaaa aaccatgaag tatttgcgcc tgaaggacgt ccggtgtaaa
    10801 tccgtcttca tcgcgcaatt cacccacgct gacatcaaca tctgtctgac cttcacgttt
    10861 aagaatcgct aatgaagcat aaggttgggt gaatcgttgt tcgctaccgt cttcttcttt
    10921 gatggttacg
//
LOCUS       DAJVRF010000129        10896 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJVRF010000129 DAJVRF010000000
VERSION     DAJVRF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10896
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.129"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            1..611
                     /locus_tag="OQN13_003166"
                     /pseudo
     CDS             1..611
                     /locus_tag="OQN13_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            608..1222
                     /locus_tag="OQN13_003167"
     CDS             608..1222
                     /locus_tag="OQN13_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCS1616450.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="OQN13_003168"
     CDS             complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="OQN13_003168"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCS1616451.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2997..3455
                     /gene="gpt"
                     /locus_tag="OQN13_003169"
     CDS             2997..3455
                     /gene="gpt"
                     /locus_tag="OQN13_003169"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS1616452.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3547..4791
                     /gene="frsA"
                     /locus_tag="OQN13_003170"
     CDS             3547..4791
                     /gene="frsA"
                     /locus_tag="OQN13_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCS1616453.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4849..5250
                     /gene="crl"
                     /locus_tag="OQN13_003171"
     CDS             4849..5250
                     /gene="crl"
                     /locus_tag="OQN13_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCS1616454.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="OQN13_003172"
     CDS             complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="OQN13_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCS1616455.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6632..7735
                     /gene="proB"
                     /locus_tag="OQN13_003173"
     CDS             6632..7735
                     /gene="proB"
                     /locus_tag="OQN13_003173"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCS1616456.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGNSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7747..9000
                     /gene="proA"
                     /locus_tag="OQN13_003174"
     CDS             7747..9000
                     /gene="proA"
                     /locus_tag="OQN13_003174"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCS1616457.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9115..9190
                     /locus_tag="OQN13_003175"
     tRNA            9115..9190
                     /locus_tag="OQN13_003175"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9148..9150,aa:Thr,seq:cgt)
     gene            9796..9954
                     /locus_tag="OQN13_003176"
     CDS             9796..9954
                     /locus_tag="OQN13_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616458.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2760 a         2574 c         2777 g         2785 t
ORIGIN      
        1 ctattttaca gcggcatatt gcctcgtttt cgcatcatgg tttgcctgaa ggcagtgacg
       61 acgcgctacg gtatattgcg gaacatgacg atgcgctggc gtttgcgcgt attaatcgcc
      121 agctgatctc tttacgcata atgcaacagg ttaaggccac cggaagtccg gttctggatg
      181 tggcgcataa ctttgttagc gcgtgtcaaa tcggcgatca acagggctgg ttgcatcgta
      241 aaggtgccac gccggatgac aacggtctgg tgattattcc cggttcactc ggtgattact
      301 cctagcttgt acagcctgtt gcgaacgaaa aaacgctcca ctcgctggcg catggggctg
      361 ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc agcgaaatac acagcgacgc
      421 agctctcacg tactgaactt ggcagtcggg taatttgtcg cgataaacaa ctcatctttg
      481 aagaagcgcc acaagcctat aaatcggctg aaagcgtggt gcaatgtctg gtgcaggctg
      541 gtttaattat tcctgtcgcg cggctgcgtc cggtgctaac gctcaaaaac agtggaggga
      601 aaaaaggatg atcttgctac aactctcctc agcgcagggg ccggaagaat gttgtctcgc
      661 agtgaaaaag gcgctggagc gattaattaa agaagctgcc cgacaagacg tcgcggtaac
      721 ggtgttggaa acagaaacgg gccgctactc tgacacgctg cgttcagctc tggtttctct
      781 ggatggcgat aacgcatggg cattaagcga aagctggtgc ggcactattc agtggatttg
      841 tccgagtccg tatcggcctc atcatgggcg caaaaactgg tttctgggca ttgggcgttt
      901 taccgctgat gagcaggaac aatcggatgc aatccgttat gagacgttgc gttcgtcggg
      961 gccgggcggt caacatgtca ataaaaccga ctcggcggta cgcgccacgc atttggcatc
     1021 cggtattagc gtgaaggttc agtcagagcg tagtcagcat gctaacaagc ggctggcacg
     1081 attgctgatt gcctggaagc tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc
     1141 gcagcggcga atgttccatc accagattga acgtggcaac ccgagacgga catttacagg
     1201 gatggctttt atcgaaggat aatgaaggat gaaactgccg gaaggcgatt aaacgccatc
     1261 cggcagcaaa tcaaataatt acttcgccgg aatttctttc agcagttcag tcagcagtgt
     1321 ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat
     1381 agttggcccg atagaaacca tgtccatttc cggatacggt tttttgaaca gaccacattc
     1441 caggcccgcg tggataatct ggatgttcgg cgttttgttg aacagacgct gataggtttc
     1501 acgtaccaga tgcatcaccg gagaattagc gtccggctgc cagccaggat atgcgccttt
     1561 cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc agcatgctca ccacgtagtc
     1621 tttaccgctg tcgatcagag aacggatcag acagtgaatt tctacgttat tatcagtcat
     1681 ggtcaccaca ccgacgttca gggaggtttc gaccacacct tttgccacgt ctgagttgcg
     1741 gatcacaccg ttcggggtgg cgttcagcag acgaataaag gtatcgcgag atttcgcaat
     1801 cagggcagct ttatcgttcg ctacagagtc cagcaacaag gccagatttt tctctttctc
     1861 tgccagctcg tttttcagga tctcctgata ggtattcacc agagatttca ggacgtcgac
     1921 tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt
     1981 gccgccgttg aaatcgataa ggcgcagatc cagttcttct gcatgacccg ctaggaagcg
     2041 caccagcagt ttgttggcat tacccaggcc aacgtggatt tccccgccgg agtgaccccc
     2101 tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc
     2161 taaatgcagg ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc
     2221 ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccgaa
     2281 cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg ggccgtgaac
     2341 cacgttttcg tcagccagaa ccgccagcgc agacgccata ccaatgccgt tatctgcacc
     2401 cagcgtggtg ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt
     2461 cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa
     2521 gacgaccgat ttacgatttt ccatacctgc ggtagcaggt ttacgaatca ggatattacc
     2581 tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc
     2641 gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa aaatatccca
     2701 cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc
     2761 gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt
     2821 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     2881 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     2941 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     3001 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     3061 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac
     3121 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     3181 gctacgatca cgacaaccag cgcgagctta aagtgctaaa acgcgcagaa ggcgatggcg
     3241 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     3301 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     3361 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     3421 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     3481 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     3541 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     3601 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     3661 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     3721 cgcggcattg acccacgtga aatcctcgac gtccaggcgc gtattgtgat gagcgatgcc
     3781 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggtgg caactggatt
     3841 tatgagtggg ccatccaggc gatggtgtgg caacaaaaag cctgtgcgga agaagatccg
     3901 caactcagtg gtcgtcactg gctgcatgcg gctacgttgt acaacattgc tgcctatcct
     3961 catctgaaag gagatgacct ggccgagcaa gcgcaggctt tgtcaaaccg tgcctatgaa
     4021 gaagccgccc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt acccggcggt
     4081 gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacagtatta
     4141 atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt
     4201 gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt ttcatcaaaa
     4261 tggaagctca cccaggactc cagcctgttg catcagcacg ttttaaaggc tctgcctaac
     4321 gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt
     4381 gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg
     4441 gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt
     4501 gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt acgcgtggag
     4561 ctgaatcgct attcattaaa agtgcaagga ttgctggggc gtcgctgccc aacgccaatg
     4621 ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacc
     4681 tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta tcggaatttt
     4741 gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta aaaatttgct
     4801 aaaatttgcc aatttggtaa aacagttgca tcacaacagg agattgcaat gacgttaccg
     4861 agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc gtatattcgt
     4921 gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg
     4981 aaaccggcac cggaagtgcg tgagttctgg ggctggtgga tggagcttga agcgcaggaa
     5041 tcccgtttta cctacagtta ccagtttggt ctgttcgata aagcaggcga ctggaagagt
     5101 gttccggtaa aagacactga agtggttgaa cgactggagc acaccctacg tgagtttcac
     5161 gagaagctgc gtgaactgct gacgacgctg aatctgaagc tggaaccggc ggatgatttt
     5221 cgtgatgaac cggtgaagtt aacggcgtga gtgaaatgtg ccggatgtat cacatccggc
     5281 aatatccatt aaaactgata ggtcatgcca accgcgacaa tatcatcatt attaatattc
     5341 aatttgttat cgctatccag ttggttgatt ttataatcaa caaacgctga catatttttg
     5401 ttgaaataat acgtagcacc gacgtcgata taattgacca gatcttcatc accgatacct
     5461 tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc aaagtcaaac
     5521 tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc agttattggc
     5581 gtcattttgc gtgtttcaga atagaaagtt gccagataaa tattattggc atcgtatttc
     5641 agacctgttg cccatgcttc tgcacgcttg cctgtgccac ggctttgcag gttctgctcg
     5701 ttggtgcgat ctgagttggt ataggcccca ctaatggcga aatcgctgcc gccaaagtca
     5761 tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg gttttcgttt
     5821 ttcccttgat attgcagggt taagttcagg ccatcgataa cgccgaagaa gtcggtgttc
     5881 cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggaa
     5941 tcgccaccaa attccgggaa catatcggtc caggcttcca cgtcatacaa cgcgcccagg
     6001 ttacgaccat aatcgaaaga acccaaatct ttatatttca acccggcaaa agcgagacgc
     6061 gttttctgct gtgcagtatc actctccgct ttatttccgg caaactccgc ttcccaccga
     6121 ccataaccag tcagttgatc gttaatttgt gtttcacctt taaaaccaaa acggatataa
     6181 ctctggtcgc catctttact ggcgttatca ctcatataat gcatagcttt aactttgcca
     6241 tagacatcca gtttattacc gtctttatta tatatttctg cagcctgtac agatgcagat
     6301 gccacaatgc ccatcaccac taatgccaga gtgctctttt tcattttcat tcctgatttt
     6361 aattaacgcg cgaatattca gcgggagagc cccgttgaaa acaggaaagt ttttaacctg
     6421 agattgttaa agatatatta cagattaata atattcttaa aatgtggtaa tttattaaat
     6481 ctgtaataaa agcgtaaaca actgccgcta ggcttgctga tcccgcgcaa caaaacgcca
     6541 tgctttgctc gcagatggtt ggcaaccgac gacagtcctg ctaaaacgtt cgtttgatat
     6601 catttttcct aaaattgaat ggcagagaat catgagtgac agccagacgc tggtggtaaa
     6661 actcggcacc agtgtgctaa caggcggatc gcgccgtctg aaccgtgccc atattgttga
     6721 acttgttcgc cagtgcgcgc agttacatgc cgccgggcat cggattgtta ttgtgacgtc
     6781 gggcgcgatc gccgccggac gtgagcacct gggttacccg gaactgccag cgactatcgc
     6841 ctcgaaacaa ctgctggcag cggtagggca gagtcgactg attcaactgt gggaacagct
     6901 gttttcgatt tatggcattc acgtcgggca aatgctgctg acccgtgctg atatggaaga
     6961 ccgtgaacgc ttcctgaacg cccgcgacac cctgcgtgcg ttgctcgata acaatatcgt
     7021 tccggtaatc aatgagaacg atgctgtcgc tacggcagag attaaagtcg gcgataacga
     7081 taacctttct gcgctggcgg cgattctggc gggtgccgat aaactgttgc tgctgaccga
     7141 tcaaaaaggt ttgtataccg ctgacccgcg cagcaatccg caggcagaac tgattaaaga
     7201 tgtttacggc attgatgacg cactgcgcgc gattgccggt aacagcgttt caggcctcgg
     7261 aactggcggc atgagtacca aattgcaggc cgctgacgtg gcttgccgtg cgggtatcga
     7321 caccattatt gccgcgggca gcaagccggg cgttattggt gatgtgatgg aaggcatttc
     7381 cgtcggtacg ctgttccatg cccaggcgac tccgcttgaa aaccgtaaac gctggatttt
     7441 cggtgcgccg ccggcgggtg aaatcacggt agatgaaggg gcaactgccg ccattctgga
     7501 acgcggcagc tccctgttgc cgaaaggcat taaaagcgtg actggcaatt tctcgcgtgg
     7561 tgaagtcatc cgcatttgca acctcgaagg ccgcgatatc gcccacggcg tcagtcgtta
     7621 caacagcgat gcattacgcc gtattgccgg acaccactcg caagaaattg atgcaatact
     7681 gggatatgaa tacggcccgg ttgccgttca ccgtgatgac atgattaccc gttaaggagc
     7741 tggccgatgc tggaacaaat gggcattgct gcgaagcaag cctcgtataa attagcgcaa
     7801 ctctccagcc gcgaaaaaaa tcgcgtgctg gaaaaaatcg ccgatgaact ggaagcacaa
     7861 agcgaaatca tcctcaacgc taacgcccag gatgttgctg acgcgcgagc caatggcctt
     7921 agcgaagcga tgcttgaccg tctggcactg acgcccgcac ggctgaaagg cattgccgac
     7981 gatgtacgtc aggtgtgcaa cctcgccgat ccggtggggc aggtaatcga tggcggcgta
     8041 ctggacagcg gcctgcgtct tgagcgtcgt cgcgtaccgc tgggggttat tggcgtgatt
     8101 tatgaagcgc gcccgaacgt gacggttgat gtcgcttcgc tgtgcctgaa aaccggtaat
     8161 gcggtgatcc tgcgcggtgg caaagaaacg tgtcgcacta acgctgcaac ggtggcggtg
     8221 attcaggacg ccctgaaatc ctgcggctta ccggcgggtg ccgtgcaggc gattgataat
     8281 cctgaccgtg cgctggtcag tgaaatgctg cgtatggata aatacatcga catgctgatc
     8341 ccgcgtggtg gcgctggttt gcataaactg tgccgtgaac agtcgacaat cccggtgatc
     8401 acaggtggta taggcgtatg ccatatttac gttgatgaaa gtgtagagat cgctgaagca
     8461 ttaaaagtga tcgtcaacgc gaaaactcag cgtccgagca catgtaatac ggttgaaacg
     8521 ttgctggtga ataaaaacat cgccgatagc ttcctgcccg cattaagcaa acaaatggcg
     8581 gaaagcggcg tgacattaca cgcagatgca gctgcactgg cgcagttgca ggcaggccct
     8641 gcgaaggtgg ttgctgttaa agccgaagag tatgacgatg agtttctgtc attagatttg
     8701 aacgtcaaaa tcgtcagcga tcttgacgat gccatcgccc atattcgtga acacggcaca
     8761 caacactccg atgcgatcct gacccgcgat atgcgcaacg cccagcgttt tgttaacgaa
     8821 gtggattcgt ccgctgttta cgttaacgcc tctacgcgtt ttaccgacgg cggccagttt
     8881 ggtctgggtg cggaagtggc ggtaagcaca caaaaactcc acgcgcgtgg cccaatgggg
     8941 ctggaagcac tgaccactta caagtggatc ggcattggtg attacaccat tcgtgcgtaa
     9001 ataaaaccgg gtgatgcaaa agtagccatt tgattcataa ggccattgac gcatcgcccg
     9061 gttagtttta accttgtcca ccgtgattca cgttcgtgaa catgtccttt cagggccgat
     9121 atagctcagt tggtagagca gcgcattcgt aatgcgaagg tcgtaggttc gactcctatt
     9181 atcggcacca ttaagaaaat taataactta gcttaacttc tctaagaaat tgtatcttct
     9241 gttatcactt acaaacgcct atacgttgtt atgggctttg gtagtgtaac acgtatgggc
     9301 gagtagttcc caggctcaac ctcgtaacaa gttcctttga agctggaaga cgtgccaccg
     9361 tctctaaacc ttctaccaaa actcttagca agatgttcca cacctggcgc ggtgtccaat
     9421 gatgctacac gtcgtgagac gttgcagagg ataaacaggc taaatgatgg gaagagtttt
     9481 aagtgcaata gtggattaaa gcccacctct tcaaaaccta acttgatcgg gaaagaatca
     9541 agctctagca cggtttcgtc caccggagta caacgggtgc ctgataagag aagaactgct
     9601 aagacttcat agcttttgtg aaaaagccct ttttagtgga tacgcctacg gcgcttattt
     9661 tttggtataa ccatcaaagg cgctttatgg ctcctattgt cttgaaaatg tatctttact
     9721 tgtattgtgt tttatgcgta gtctccctgt gtagtatgta acttgttaat tttcatatga
     9781 atacagaggc acacgatgaa aaacattgca gcaatcaaac gtaataaccg caagattcac
     9841 gctcgtaagt tcctgtctac gccagaagga aaagcctggc tagagcgtaa acagagagag
     9901 aacgaagaaa gaaaactcct tagtgagttg aaatggctta gagaggattt ctgaaaatct
     9961 ccagaacgca acaggacgca ttctaacgct gttttcatta ctcggtatag ggataggtga
    10021 tgtgtgttta atgcgtctgt gcgcttgttt tgtgccttat tttgcatttt cataatgcgg
    10081 aagagaacac ggaaaacctt aaaattaatt attggggtat ttaaggctat tttcaggtga
    10141 tttaagggta ttaaacttga tttaattgat taaaatataa acagataatt atgtatctta
    10201 ttgatttata aggttgtttt taagtaaagg taaataaaaa ataaatagcc ataaatagct
    10261 ataaatacat cttaatacct atatcccttc ccttatggct agattgcgag aggtgcttaa
    10321 cgcgattttc agcgcccccc gatctggaaa atccagaatc cccaaatatc agcaaggaat
    10381 tttcttgcgt aatcaacatc gtttttacct attaaatcat tacacagaaa gcctttctgt
    10441 aatgtctctc actaaacttt cactaaacac gagaatgatc ggttttatgt cggatcagat
    10501 gctagaaaca gcaccacgtc tcacaagggc tgtaagcgat gaaaccagtg tttatgcggg
    10561 tgcaggtcaa aatataggcc aaaatccatt taacataata aatgttatat gtgctaagga
    10621 acacatataa cgccttgaat tgatgtatca gtggaaaagt gatggatttt ttacgtagga
    10681 gcacgaaagg gcttctattg acttttgtgt gctatgaaat ataattaaag ttttttgttt
    10741 ttattgtgtg atgtggtggg ttacacaggg gaatagatta aatggtgttc tgtttataca
    10801 ggtttatttg ttagatcaac aatttggcat ttctaaacag atgtttgaac gttgccaaat
    10861 gtcttgacaa cacaagaaaa gggcttgcta tgccag
//
LOCUS       DAJVRF010000130        10875 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJVRF010000130 DAJVRF010000000
VERSION     DAJVRF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10875
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.130"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            109..450
                     /locus_tag="OQN13_003177"
     CDS             109..450
                     /locus_tag="OQN13_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCS1616459.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(441..1367)
                     /locus_tag="OQN13_003178"
     CDS             complement(441..1367)
                     /locus_tag="OQN13_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1616460.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGAPLYQI"
     gene            1457..2455
                     /locus_tag="OQN13_003179"
     CDS             1457..2455
                     /locus_tag="OQN13_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCS1616461.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2452..2670)
                     /locus_tag="OQN13_003180"
     CDS             complement(2452..2670)
                     /locus_tag="OQN13_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCS1616462.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2672..4687)
                     /gene="ligA"
                     /locus_tag="OQN13_003181"
     CDS             complement(2672..4687)
                     /gene="ligA"
                     /locus_tag="OQN13_003181"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCS1616463.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4758..5744)
                     /gene="zipA"
                     /locus_tag="OQN13_003182"
     CDS             complement(4758..5744)
                     /gene="zipA"
                     /locus_tag="OQN13_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCS1616464.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            5974..6735
                     /gene="cysZ"
                     /locus_tag="OQN13_003183"
     CDS             5974..6735
                     /gene="cysZ"
                     /locus_tag="OQN13_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCS1616465.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6920..7891
                     /gene="cysK"
                     /locus_tag="OQN13_003184"
     CDS             6920..7891
                     /gene="cysK"
                     /locus_tag="OQN13_003184"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCS1616466.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8275..8532
                     /gene="ptsH"
                     /locus_tag="OQN13_003185"
     CDS             8275..8532
                     /gene="ptsH"
                     /locus_tag="OQN13_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCS1616467.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8577..10304
                     /gene="ptsI"
                     /locus_tag="OQN13_003186"
     CDS             8577..10304
                     /gene="ptsI"
                     /locus_tag="OQN13_003186"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCS1616468.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10345..10854
                     /gene="crr"
                     /locus_tag="OQN13_003187"
     CDS             10345..10854
                     /gene="crr"
                     /locus_tag="OQN13_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCS1616469.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         2643 a         2802 c         2697 g         2733 t
ORIGIN      
        1 cgccctaaag gcgctttttt gctatctgcg atttgtaaga ttgatttttc ttctcaagtt
       61 ccttgttctt ctgccgatat tacctcgtac agtgacgagg actgaacaat ggccactatc
      121 tcttcaactt caattccttc catacaaaca cagagtagta acagggcatc acaaggtagc
      181 gatgttgctt caaaaatcgc gcggataagt cagcaaatca tcaaactcac tcaacaaatt
      241 aaagagattg tagacactag cggcagtgct gaagacaaac aaaaacaggc cgaattgatt
      301 caacagcaaa tcaccctact ggaaacacaa ctggcgcagt tgcagagaca gcaggcagaa
      361 aaggcgcagg agaaagagca gcgtttgtct ctgaatgtat cccttctaaa tcctgttgaa
      421 aatacgactc atatcgatat ctatatctga tacaacggag ccccttcccg accagccatt
      481 agcgcattgc cctggtctcg taccacgtcc cataacgctt cagcggcggt ggaaagtgaa
      541 cggttcttcc tgcgcaccag catcaactgg cgttcaacaa ccggcgtaat gcgctttacc
      601 accagtgggc taccttcagg caatggtagc gccagtgcgg gaagaatact gatgccgatg
      661 cctgcggcta ccatcgggaa cagcgtcgcc ggatgtccaa tctcctgcac aatattcgcc
      721 tgaataccat tgcgcgccag cgctgcgtca atcagcggtc ggctgcctga cgcgtaatcc
      781 tgcaacacca atttcgctcc ctgtaatgcc tgccagggta cataatcttc cacggccaaa
      841 gcgctatcgc gatggcaaag cagaaagaaa ggctcggaaa gaatcgcttc acattgcaga
      901 tcgcccactg ggcctggatc gatgacgatg caaaaatcga catctccctg gcgaatactt
      961 tccatcaccc actgctgcgg acgatcgtgc aggacaaact gaatatctgg atagcggcga
     1021 tggctttcag caatgcattg cggaataaga tgcgcggaaa tggtctggct ggcagcgacc
     1081 cgaacttttc cgctcagttg ttgccccata cgcccggtat cgcgcaacgt gctgttaagt
     1141 tcatccagca gtcgctcaag acgcaaagcc aactgctgcc ctgcatctgt aagcaccact
     1201 tcacgcgtgg ttctgtccag caggcgaaca ccagtatgat tttccagctc cttcacactg
     1261 tgacttactg ccgactggct caagccgata cgctctcctg cacgactaaa acttttctcc
     1321 tgcgctactg tgacgaaaac ctttaattgt tttaaagaat aattcatctg ttttcttcat
     1381 gaatggatgc aataaatcaa ttttatttct caaactggaa gaagcacaat agaaccatcg
     1441 atcatctgga gtctttatga aactttttcg tatcctcgat cctttcacct taaccctgat
     1501 cacggtggtg ttgctggcct ctttctttcc ggccagaggc gatttcgtcc ccttctttga
     1561 aaatctgacc accgcagcta ttgccctgct gttctttatg cacggcgcga agttgtcgcg
     1621 tgaggcgatt attgctggcg gtggtcactg gcgactgcat ttgtgggtaa tgtgcagcac
     1681 cttcgtgctg tttccgattc tgggtgtact gtttgcctgg tggaaaccgg taaatgtcga
     1741 cccgatgctc tactccggtt ttctctactt gtgcattctc ccggctaccg tgcagtctgc
     1801 aatcgccttc acgtcaatgg cgggcggtaa cgtcgcggcg gcggtttgtt ctgcgtcagc
     1861 atccagcctg ctggggattt tcctttcacc attgctagtt ggtctggtga tgaatgttca
     1921 cggtgcaggg ggcagccttg agcaggtcgg taaaattatg ctgcaactgc tgctgccgtt
     1981 tgtgttgggg catctttccc ggccgtggat tggtgactgg gtgtcgcgca ataaaaaatg
     2041 gattgcgaaa actgaccaga cgtccattct gttggtggtt tatacagcgt tcagcgaagc
     2101 cgtcgttaat ggtatctggc ataaagttgg ctggggatca ttgctgttta tcgtggtggt
     2161 cagctgcgtt cttctggcta tcgtgattgt agttaacgtc tttatggcac gccgactggg
     2221 cttcaataag gcagatgaaa ttactatcgt cttttgtggt tcgaaaaaga gtctggcaaa
     2281 tggcatcccg atggcaaaca ttctgttccc cacatcggtg atcggtatga tggtgctgcc
     2341 cctgatgatt ttccatcaga tccaattgat ggtctgtgcg gtgctggcgc gtcgatacaa
     2401 acgccagacc gaacagttac aggcgcagca ggaaagcagc gccgataaag cttaaagcgg
     2461 acgcttcagg ggctggacca gttgcgtcag cccctcggtt ttaatcagca gcgtgatttg
     2521 cattagctca ccgagcttcc ctgccgggaa ctcatctttg cgggcaaacc acagcagata
     2581 ctcctccggc aggtcgatta agcgacgccc tttgtatttg ccaaacggca ttatcgtatt
     2641 ggctatttca atcagctgct ctttttccat ctcagctacc cagcaaacgc agcatttccg
     2701 cttcgtcgat gacttcaatg cccagttcct gcgccttcgc cagtttagat cctgcagctt
     2761 cacccgctat caccagatcg gttttcttcg acacgctgcc cgcgactttc gcgcccagtt
     2821 cgaccagtcg agctttagcg tcatcacgcg acatctggct taagctgccc gtaagcacca
     2881 cggttttacc agcaaacggg ctgtcaatct cttccgcgtt gataacgatc ggcgcaggcc
     2941 agtgaacacc ttccgccaac agctcgctga tgacattgcg gttgctttct tcggcaaaga
     3001 agttgtgaac gtgggatgca acgacaatgc caacatcagg caccttttgc agctcttcaa
     3061 tcgaagcggc ttccagcact tccagcgtgc cgaaatatgc cgccagacct gctgcggtgg
     3121 cctcgccgac ttcacggatg ccaagtgcat agaggaagcg agcaaaggtg gtttctttcg
     3181 ctttttccag cgcgttaacc acgttttgtg ccgattttgg ccccatacgc tccagtccgg
     3241 tcagtttgcc tgcggtgagt ttgaacagat ctgccggagt gtggacatat tctttttcaa
     3301 ccagctgatc gatgattttg tcgcccattc cgtcaacatc catcgcacgg cgggaaacaa
     3361 agtgtttcag cgactcttta cgctgcgcac cgcaaatcag gccaccggta cagcgggcaa
     3421 ccgcttcacc ttccacacgc tcaacgtcag aaccacatac cggacaatgc gtcgggaata
     3481 caacctcacg ggtatcttcc gggcgttcag aaagcacgac gttaaccacc tgcgggatca
     3541 cgtcgccagc gcggcgaatc accactttat cgccaatgcg taaaccaaga cgttcgattt
     3601 catccgcatt gtgtaaggtt gcgttactca ccagcacgcc agcaacatgg acaggttcca
     3661 gacgcgcaac aggcgtaatc gcgccagtac gcccaacctg aaactcgacg tcacgcacaa
     3721 aggtcatctg ctcctgcgcc gggaatttaa acgctaccgc ccagcgcggg gcacgcgcga
     3781 caaagccaag ctgctcctgc tgttccagtg agttgacctt aatcaccacg ccgtcgatat
     3841 caaagcccag cgtcgggcgg tcttcttcca ctttgtgata gaacgccagc acttcttccg
     3901 ccgattcaca aagcgttacc cgatcgctga ccggcaaccc ccacttttta aattgcagta
     3961 aacggccaag atgagtatcc ggcagctcgc caccttccag aacaccaacg ccatagcaga
     4021 aaaaagtgag cggtcgcttc gctgtaatac gcggatcaag ctgacgcagt gaaccagctg
     4081 ccgcattacg tgggttagca aaaactttcc cgcccgtgcg tcgcgcatct tcgttaatct
     4141 tttcgaaccc cgcctgcggc aggaacactt caccacgcac ttccagacgc gccgggatat
     4201 tctctccgtg cagcttcagc ggaatggcgc gaatagtacg cacattagac gtgatatctt
     4261 ccccggtggt gccatcgcca cgggtcgcgg cactgactaa aacgccattt tcatacagaa
     4321 tactgacggc aaggccatcc agcttcagct cacagcacca ggtgactttc tcgttgtttt
     4381 tcagacggtc ctgcacacgt ttgttgaaag caagaaagct ttcttcatca aaaacgttat
     4441 ccagtgacag cattggtact tcatggcgta tctggctgaa agccgccagc ggcgcagcac
     4501 ctacacgttg ggtaggcgaa tcaggcgtaa tcagttctgg atgtttggtt tccagctcgc
     4561 gcagttcgcg catcagcctg tcgtattcag cgtcgggaat ttccggcgca tccatcacat
     4621 gataaagata ttcatgatgg cgaagcgtcg ttcgcagttc tgtcagttgt tgttcgattg
     4681 attccatatc gcaccatcaa tgctaaaaac ccccgacaag cgggggttcg aagaggagtt
     4741 aatttgcctt aagtgtatca ggcgttggcg tctttgactt cgcggatgat gtcctggtac
     4801 tcgcgcaatt tctgcggagt catcatacgg cgctgatcgt caagcacgac accgcccact
     4861 tcatcggcaa tatgctgcgc agattgcagc atcagcttga agttctgcag ctcgtcaccg
     4921 taagacggta cctgcataaa gatagtgaca cccggagtag tgaaatcctt catttcagga
     4981 tcaaaggttc ccggtttcac catattcgcc aggctgaata acgccgggcc gctgccatcc
     5041 gggctaagat gacgatggta aatattcata tcgccaaaaa tgaagcccgc ttgttgaatg
     5101 ctgttaagaa gcagttcacc gtttagctcg ctaccgtgat gcgccgcgac gttcatgata
     5161 atcaccgctt ctttgcgctt cggtttatcc ataactggag ccggttccgc tacaggctca
     5221 ggctgtggtg ccgctacggg ttctgcaggc tggaaagcct gttgtgccgg ttgcggtgct
     5281 gaatgcacag gctgcggcgc tggcgcgacc tgtggcgaaa ctggctgctg caacggctgt
     5341 tcaggctgcg gctgataggc aggctgctgc atcggctgcg ctggacgcgg agcatgttgc
     5401 ggcggtacct gcgcttcagg cggctgctgg atcggttgac gcggctgcgc agacgcataa
     5461 ggcggttggt actggtgttg cggcgacgga cgagcagcct catgctcctg agcgttagcc
     5521 ggggcatgat tcacgcggtg aacacgaacc tcaccaacgc cctcatcatc ttcgacatcc
     5581 tcgtcataag aatcgtcgtc acgttttgac ttcattcgtt ttaatggccg atcgcggaac
     5641 atagaagatc gttctttacg gctggtccag aaaccatgta ccagtaaagc gattatggcg
     5701 atcgcgccaa caatgattaa tatcagacgc aaatcctgca tcattatatt ctctgttgtt
     5761 ctaacacctt gccaccacgg caaacattta ctcactaaga gtatttgccg attacctcaa
     5821 gtgcaagtgc actattaact ttcacagcac aaagatagat gaaatcgtgc tttttgctgt
     5881 tttttcgaac atatcctaac tgtccattgc gcaattaccc ggtaaaatac gcagaatttt
     5941 cctgggattg gtcaaaagga gctcatcctg attatggttt catcattcac atctgcccca
     6001 cgcagcggtt tttactattt tgcgcaaggc tggaagctcg tctcgcaacc tgggattcgg
     6061 cgtttcgtta ttttaccgct gctggtcaat attttgttga tggggggcgc attctggtgg
     6121 ctctttacac agctcgatgt ctggatcccg actctcatga gttacgttcc ggactggcta
     6181 caatggctga gttatctgtt gtggcctctg gctgtcatct ctgtgctgtt agtgtttggc
     6241 tatttcttct ccacgattgc taactggatt gccgctccgt ttaacggttt attggctgaa
     6301 caactggaag cacgattgac tggcgctaca ccgccagata ccgggatttt cggtatcatg
     6361 aaagatgtgc cgcgaatcat gaaacgcgaa tggcaaaaat ttgcctggta tctgccgcgc
     6421 gcaattgtat tactaattct ttacttcatc cctggtattg ggcaaaccgt cgcgccggta
     6481 ctgtggttcc tgtttagcgc ctggatgtta gccatccagt attgcgatta ccccttcgat
     6541 aaccacaaag tgccgtttaa agagatgcgc accgccctgc gcacacgcaa aatcaccaat
     6601 atgcagtttg gtgctttaac cagcctgttt acgatgatcc cgctgcttaa tctgttcatc
     6661 atgcccgttg ccgtttgtgg cgcgacggcg atgtgggtcg attgctatcg cgataaacac
     6721 gcgatgtggc ggtaacaatc taccggttat tttgtaaacc gtttgtgtga aacaggggtg
     6781 gcttatgccg ccccttattc catcttgcat gtcattattt cccttctgta tatagatatg
     6841 ctaaatcctt acttccgcat attctctgag cgggtatgct acctgttgta tcccaatttc
     6901 atacagttaa ggacaggcca tgagtaagat ttttgaagat aactcgctga ctatcggtca
     6961 cacgccgctg gttcgcctga atcgcatcgg taacggacgc attctggcga aggtggaatc
     7021 tcgtaacccc agcttcagcg ttaagtgccg tatcggtgcc aacatgattt gggatgccga
     7081 aaagcgcggc gtgctgaaac caggcgttga actggttgaa ccgaccagcg gtaataccgg
     7141 gattgcactg gcctatgtag ctgccgctcg cggttacaaa ctcaccctga ccatgccaga
     7201 aaccatgagt attgaacgcc gcaagctgct gaaagcgtta ggtgcaaacc tggtgctgac
     7261 ggaaggtgct aaaggcatga aaggcgcaat ccaaaaagca gaagaaattg tcgccagcaa
     7321 tccagagaaa tacctgctgc tgcaacaatt cagcaatccg gcaaaccctg aaattcacga
     7381 aaaaaccacc ggtccggaga tctgggaaga taccgacggt caggttgatg tatttattgc
     7441 tggcgttggg actggcggta cgctgactgg cgtcagccgc tacattaaag gcaccaaagg
     7501 caagaccgat cttatctctg tcgccgttga gccaaccgat tctccagtta tcgcccaggc
     7561 gctggcaggt gaagagatta aacctggccc gcataaaatt cagggtattg gcgctggttt
     7621 tatcccggct aacctcgatc tcaagctggt cgataaagtc attggcatca ccaatgaaga
     7681 agccatttct accgcccgtc gtctaatgga agaagaaggt attcttgcag gtatctcttc
     7741 tggagcagct gttgccgcgg cgttgaaact acaagaagat gaaagcttta ccaataagaa
     7801 tattgtggtt attctaccat catcgggtga gcgttattta agcaccgcat tgtttgccga
     7861 tctcttcact gagaaagaat tgcaacagta atgccagctt gttaaaaatg cgtaaaaaag
     7921 caccttttta ggtgcttttt tgtggcctgc ttcaaacttt cgcccctcct ggcattgatt
     7981 cagcctgtcg gaactggtat ttaaccagac taattatttt gatgcgcgaa attaatcgtt
     8041 acaggaaaag ccaaagctga atcgatttta tgatttggtt caattcttcc tttaacggca
     8101 taatgtttaa tgacgtacga aacgtcagcg gtcaacaccc gccagcaatg gactgtattg
     8161 cgctcttcgt gcgtcgcgtc tgttaaaaac tggcgctaac aatacaggct aaagtcgaac
     8221 cgccaggcta gactttagtt ccacaacact aaacctataa gttggggaaa tacaatgttc
     8281 cagcaagaag ttaccattac cgctccgaac ggtctgcaca cccgccctgc tgcccagttt
     8341 gtaaaagaag ctaagggctt cacttctgaa attactgtga cttccaacgg caaaagcgcc
     8401 agcgcgaaaa gcctgtttaa actgcagact ctgggcctga ctcaaggtac cgttgtgact
     8461 atctccgcag aaggcgaaga cgagcagaaa gcggttgaac atctggttaa actgatggcg
     8521 gaactcgagt aatttcccgg gttcttttaa aaatcagtca caagtaaggt agggttatga
     8581 tttcaggcat tttagcatcc ccgggtatcg ctttcggtaa agctctgctt ctgaaagaag
     8641 acgaaattgt cattgaccgg aaaaaaattt ctgccgacca ggttgatcag gaagttgaac
     8701 gttttctgag cggtcgtgcc aaggcatcag cccagctgga aacgatcaaa acgaaagctg
     8761 gtgaaacgtt cggtgaagaa aaagaagcca tctttgaagg gcatattatg ctgctcgaag
     8821 atgaggagct ggagcaggaa atcatagccc tgattaaaga taaacacatg acagctgacg
     8881 cagctgctca tgaagttatc gaaggtcagg cttctgccct ggaagagctg gatgatgaat
     8941 acctgaaaga acgtgcggct gacgtacgtg atatcggtaa gcgcctgctg cgcaacatcc
     9001 tgggcctgaa gattatcgac ctgagcgcca ttcaggatga agtcattctg gttgccgctg
     9061 acctgacgcc gtccgaaacc gcacagctga acctgaagaa ggtgctgggt ttcatcaccg
     9121 acgcgggtgg ccgtacttcc cacacctcta tcatggcgcg ttctctggaa ctgcctgcta
     9181 tcgtgggtac cggtagcgtc acctctcagg tgaaaaatga cgactatctg attctggatg
     9241 ccgtaaataa tcaggtttac gtcaatccaa ccaacgaagt tattgataaa atgcgcgctg
     9301 ttcaggagca agtggcttct gaaaaagcag agcttgctaa actgaaagat ctgccagcta
     9361 ttacgctgga cggtcaccag gtagaagtat gcgctaacat tggtacggtt cgtgacgttg
     9421 aaggtgcaga gcgtaacggc gctgaaggcg ttggtctgta tcgtactgag ttcctgttca
     9481 tggaccgcga cgcgctgccc actgaagaag aacagtttgc tgcttacaaa gcagtggctg
     9541 aagcgtgtgg ctcgcaggcg gttatcgttc gtaccatgga catcggcggc gacaaagagc
     9601 tgccatacat gaacttcccg aaagaagaga acccgttcct cggctggcgc gctatccgta
     9661 tcgcgatgga tcgtaaagag atcctgcgcg atcagctccg cgctatcctg cgtgcctcgg
     9721 ctttcggtaa attgcgcatt atgttcccga tgatcatctc tgttgaagaa gtgcgtgcac
     9781 tgcgcaaaga gatcgaaatc tacaaacagg aactgcgcga cgaaggtaaa gcgtttgacg
     9841 agtcaattga aatcggcgta atggtggaaa caccggctgc cgcaacaatt gcacgtcatt
     9901 tagccaaaga agttgatttc tttagtatcg gcaccaatga tttaacgcag tacactctgg
     9961 cagttgaccg tggtaatgat atgatttcac acctttacca gccaatgtca ccgtccgtgc
    10021 tgaacttgat caagcaagtt attgatgctt ctcatgctga aggcaaatgg actggcatgt
    10081 gtggtgagct tgctggcgat gaacgtgcta cacttctgtt gctggggatg ggtctggacg
    10141 aattctctat gagcgccatt tctatcccgc gcattaagaa gattatccgt aacacgaact
    10201 tcgaagatgc gaaggtgtta gcagagcagg ctcttgctca accgacaacg gacgagttaa
    10261 tgacgctggt taacaagttc attgaagaaa aaacaatctg ctaatccacg agatgcggcc
    10321 caatttactg cttaggagaa gatcatgggt ttgttcgata aactgaaatc tctggtttcc
    10381 gacgacaaga aggataccgg aactattgag atcattgctc cgctctctgg cgagatcgtc
    10441 aatatcgaag acgtgccgga tgtcgttttt gcggaaaaaa tcgttggtga tggtattgct
    10501 atcaaaccaa cgggtaacaa aatggtcgcg cctgtagacg gtaccattgg taaaatcttt
    10561 gaaaccaatc acgcgttctc tatcgaatct gatagcggcg ttgaactgtt cgtccacttc
    10621 ggtatcgaca ccgttgaact gaaaggcgaa ggcttcaagc gtattgctga agaaggtcag
    10681 cgcgtgaaag ttggcgatac tgtcattgaa tttgatctgc cgctgctgga agagaaagcc
    10741 aagtctaccc tgactccggt tgttatctcc aacatggacg aaatcaaaga actgatcaaa
    10801 ctgtccggta gcgtaaccgt gggtgaaacc ccggttatcc gcatcaagaa gtaattcttg
    10861 ccgcagtgaa aaatg
//
LOCUS       DAJVRF010000131        10852 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJVRF010000131 DAJVRF010000000
VERSION     DAJVRF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10852
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.131"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..1022
                     /gene="narU"
                     /locus_tag="OQN13_003188"
     CDS             <1..1022
                     /gene="narU"
                     /locus_tag="OQN13_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCS1616470.1"
                     /translation="PNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAKQGSALGI
                     NGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWVPLLAIAT
                     IAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQF
                     PDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLFLTLPGTG
                     SGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQAQKEAITE
                     TAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVYGRR
                     KFSQK"
     gene            1104..4844
                     /gene="narZ"
                     /locus_tag="OQN13_003189"
     CDS             1104..4844
                     /gene="narZ"
                     /locus_tag="OQN13_003189"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HCS1616471.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            4841..6385
                     /gene="narH"
                     /locus_tag="OQN13_003190"
     CDS             4841..6385
                     /gene="narH"
                     /locus_tag="OQN13_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCS1616472.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6385..7080
                     /gene="narW"
                     /locus_tag="OQN13_003191"
     CDS             6385..7080
                     /gene="narW"
                     /locus_tag="OQN13_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCS1616473.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7077..7757
                     /gene="narI"
                     /locus_tag="OQN13_003192"
     CDS             7077..7757
                     /gene="narI"
                     /locus_tag="OQN13_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCS1616474.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            7836..8729
                     /locus_tag="OQN13_003193"
     CDS             7836..8729
                     /locus_tag="OQN13_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HCS1616475.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(8825..9670)
                     /gene="nhoA"
                     /locus_tag="OQN13_003194"
     CDS             complement(8825..9670)
                     /gene="nhoA"
                     /locus_tag="OQN13_003194"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HCS1616476.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            9843..10412
                     /locus_tag="OQN13_003195"
     CDS             9843..10412
                     /locus_tag="OQN13_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCS1616477.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(10416..10643)
                     /gene="pptA"
                     /locus_tag="OQN13_003196"
     CDS             complement(10416..10643)
                     /gene="pptA"
                     /locus_tag="OQN13_003196"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HCS1616478.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
BASE COUNT         2528 a         2837 c         2926 g         2561 t
ORIGIN      
        1 atccgaatac tccttttggg atatttatcg ttatcgcttt gctatgcggt tttgcaggtg
       61 caaactttgc ttcgagcatg ggcaatatca gtttcttctt tccaaaagcc aaacaaggga
      121 gcgctcttgg gattaatggc ggattaggaa acttaggtgt aagtgtaatg cagctggttg
      181 caccgctggt catttttgta cctgtatttg cctttctcgg cgtcaatggc gtaccgcagg
      241 ccgacggttc ggtgatgtcg ctggcgaatg ccgcatggat ttgggtaccg ctactggcga
      301 ttgccacgat cgccgcctgg tcagggatga atgatatcgc cagttcacgc gcctcaattg
      361 acgaccagct ccctgtctta caacgcctgc atctctggct gctgagcctg ctttaccttg
      421 ccaccttcgg ttcgtttatc ggtttttctg cgggttttgc catgctggca aaaacccagt
      481 tcccggatgt gaatattctg cgcctggcgt tctttggccc atttatcggt gccatcgcgc
      541 ggtcggttgg tggtgctatt tccgataaat tcggcggcgt gcgggtgacg ttgatcaact
      601 ttatttttat ggcgattttc agtgccctgc tgttccttac cttaccgggc acaggctccg
      661 gtaatttcat cgccttttac gccgtattta tggggctgtt tctgactgcg ggtctgggaa
      721 gtggttctac tttccagatg atcgccgtca tctttcgcca gataaccatt tatcgggtga
      781 agatgaaagg cggtagtgat gagcaagctc agaaagaagc catcaccgaa acggcggcgg
      841 ctctgggctt tatctcagcc attggcgcag tgggcggctt ttttattccg caggcgtttg
      901 gcatgtcgct caatatgacc ggctctccgg tcggcgcgat gaaagtgttt ttaatcttct
      961 acatcgtttg tgtgctgctg acctggctgg tttatggtcg gcggaagttc agccaaaaat
     1021 aagagatcga tgcctgatgt acaaccgtgt cgcatccgac aaggcatcag atcccaattc
     1081 attcctggag caggagtcat gtcatgagta aacttttgga tcgctttcgc tacttcaaac
     1141 aaaagggcga aacctttgcc gatggtcacg gacaggtgat gcatagcaac cgcgactggg
     1201 aggacagcta tcgccagcgt tggcagttcg acaaaattgt gcgttccacc cacggtgtta
     1261 actgtacagg ctcctgtagc tggaaaatct acgttaaaaa tggtctggtg acctgggaaa
     1321 tccaacagac cgactacccg cgcactcgcc ctgaccagcc caatcatgaa cctcgcggct
     1381 gcccgcgtgg cgcaagttac tcctggtatc tctacagcgc taaccgcctg aaatacccgc
     1441 tcattcgtaa acgactgatt gaactgtggc gtgaagctct caagcaacac agcgatccgg
     1501 tactggcgtg ggcatcgatt atgaacgatc cgcaaaagtg cttaagctac aaacaagtgc
     1561 gtgggcgcgg cgggtttatc cgctccaact ggcaggaact taaccaactg attgccgccg
     1621 ctaacgtctg gaccatcaaa atctatggtc cggatcgcgt tgccggtttc tcgccgatcc
     1681 cggcgatgtc gatggtgtca tacgctgccg gaacgcgtta tctgtcgctg ttaggcggca
     1741 cctgtttaag cttctacgac tggtattgcg acctgccgcc cgcctcgccg atgacctggg
     1801 gcgaacaaac cgacgtgccg gaatctgccg actggtataa ctccagttac atcatcgcct
     1861 gggggtctaa cgtaccgcag acacgtacgc cggacgccca cttctttacc gaagtacgtt
     1921 acaaaggcac caaaactatc gccattaccc ctgactactc tgaagtggcc aaattgtgcg
     1981 accagtggct ggcaccgaaa caaggcactg atagcgccct ggcgatggca atgggccatg
     2041 tgattttaaa agagtttcat ctcgataatc ccagcgacta ctttatcaac tactgccgcc
     2101 gctatagcga catgccgatg ctggtaatgc tggaacctcg cgacgatggt agctacgtac
     2161 ccgggcgcat gatccgcgcg tctgacctgg tagatggact aggcgaaagc aataatccgc
     2221 agtggaaaac cgtggcggtt aataccgcag gtgaattggt agtgccgaat ggttcgattg
     2281 gtttccgctg gggagaaaaa ggcaaatgga atctggaatc cattgccgcc ggtaaggaaa
     2341 ccgaattgtc gttaaccctg ctcggtcaac atgacgctgt tgcaggcgtg gccttcccct
     2401 actttggcgg cattgaaaat ccgcattttc gcagcgtaaa acacaatccg gtgctggtgc
     2461 gccaattgcc cgttaaaaac ctgacgttag tcgatggcaa cacctgtccg gtggtcagcg
     2521 tttatgattt ggtgctggcg aattacggcc tcgatcgcgg gctggaagat gaaaacagcg
     2581 cgaaagatta cgctgaaatc aaaccgtaca ccccagcctg gggtgagcaa attaccggcg
     2641 taccgcgcca gtatattgaa accatcgccc gcgaatttgc cgataccgcc cataaaacgc
     2701 atggacgctc aatgattatc ctcggcgctg gcgtcaacca ctggtatcac atggatatga
     2761 actaccgtgg gatgatcaat atgctgatct tctgcggttg tgtcgggcaa agcggcggcg
     2821 gctgggcgca ctatgtcggt caggaaaaac tgcgcccaca aaccggctgg ttgccgctgg
     2881 cctttgcgct cgactggaac cgcccaccgc gccagatgaa cagtacctcg tttttctaca
     2941 atcattccag ccaatggcgc tatgaaaaag tctctgctca ggagttactt tcaccgcttg
     3001 ccgatgccag taagtacagc ggtcatctga ttgatttcaa cgttcgcgcc gaacgcatgg
     3061 gctggctacc ttctgcgccg cagctggggc gtaacccgct cgggattaaa gctgaagccg
     3121 acaaggccgg attatccccc acagaattta ccgcccaggc gctgaaatcg ggcgatttac
     3181 gtatggcctg cgaacaacca gatagcggca gcaatcatcc gcgtaatttg tttgtctggc
     3241 gctctaacct gcttggctcc tccggcaaag gccacgagta tatgcagaag tatctgctgg
     3301 ggaccgaaag cgggattcag ggcgaggaac tcggtgccag cgacgggatc aaaccggaag
     3361 aagtcgagtg gcaaactgcg gcgattgaag gcaagctcga cctgctggtg acgctcgact
     3421 tccgcatgtc cagtacctgc ctgttctccg atatcgttct gcccaccgcc acctggtacg
     3481 aaaaagacga tatgaacacc tcggatatgc atccgtttat tcatccgctt tctgcggcgg
     3541 tcgatccggc gtgggaatca cgcagcgact gggaaatcta caaaggtatc gccaaagcat
     3601 tttcgcaagt gtgcgtgggt catcttggca aagaaaccga cgtggtatta caacccctac
     3661 tgcatgactc tccggcagag ctctcacagc cgtgtgaagt gctcgactgg cgcaaaggcg
     3721 aatgcgatct gattccaggc aaaaccgcgc cgaatattgt ggcggtggag cgcgactacc
     3781 ctgctacgta tgaacgcttt acctcgctcg ggccattgat ggacaaactt ggcaatggtg
     3841 gtaaagggat ttcgtggaat acccaggatg aaatcgattt cctcggcaaa ctcaattaca
     3901 ccaagcgtga tggcccggca caggggcgtc cgctgattga caccgccatt gacgcttcag
     3961 aagtgattct ggcactggca ccggaaacca acggtcatgt tgcagtcaaa gcgtggcagg
     4021 cgctgggcga gatcaccggg cgcgaacata cccatctggc gctgcacaaa gaggacgaga
     4081 agattcgctt tcgcgatatt caggcgcagc cgcgtaaaat tatctccagc cccacatggt
     4141 ccggtctgga aagcgatcac gtctcctata atgcgggata caccaacgtt catgagttaa
     4201 ttccgtggcg cacgctgtcg ggacgccagc agctctatca ggatcatccg tggatgcgtg
     4261 cttttggtga aagcctggtg gcttatcgcc cgcctatcga cacccgtagc gtcagtgaga
     4321 tgcgccagat cccgccaaac ggcttcccgg aaaaagcact taacttcctg acgccgcacc
     4381 agaaatgggg cattcactca acctacagtg aaaacctgct aatgctgacg ctctctcgcg
     4441 gtggaccgat tgtctggatc agcgaaaccg atgcccgaga actgaccatt gtcgataacg
     4501 actgggtgga agtctttaac gccaacggcg cgctgacggc tcgtgcggtg gtcagccaac
     4561 gtgtaccgcc gggtatgacc atgatgtatc acgcccagga acgcattatg aatattcctg
     4621 gttcggaagt aactggcatg cgcggcggca ttcataactc ggtcacccgt gtttgcccga
     4681 aaccgacgca tatgattggc ggttacgcgc agctggcctg gggctttaac tactacggca
     4741 ccgtcggctc gaaccgcgac gaattcatca tgatccgcaa gatgaagaac gttaactggc
     4801 tggatgatga aggtcgcgat caggtacagg aggcgaaaaa atgaaaatac gttcgcaagt
     4861 cggcatggtg cttaacctcg acaaatgtat cggttgccat acctgttcgg tgacctgtaa
     4921 aaacgtctgg accggacgcg aaggcatgga gtacgcatgg tttaacaacg tcgaaaccaa
     4981 accgggcatt ggttatccga aaaactggga agatcaggaa gagtggcaag gcggctgggt
     5041 ccgcgatgtg aatggcaaga tacgcccgcg tctgggcagc aagatgggcg taataaccaa
     5101 aatcttcgcc aacccggtgg tgccgcagat tgatgattac tacgaacctt tcaccttcga
     5161 ctacgaacat ttgcatagcg caccggaagg caaacatatc cctactgctc gcccgcgttc
     5221 gctgattgac ggtaagcgga tggacaaagt gatctggggg ccaaactggg aagaactgct
     5281 gggcggcgag tttgaaaaac gtgcccgcga ccgcaacttc gaggccatgc aaaaggagat
     5341 gtacgggcag tttaaaaaca ccttcatgat gtacctgccg cgcctgtgcg aacactgcct
     5401 caatcccagt tgcgtggcga cctgcccaag cggcgctatc tacaaacgcg aagaagacgg
     5461 cattgtgctg attgatcagg ataaatgccg tggctggcgt ttgtgcatta gcggttgtcc
     5521 gtacaaaaaa atctacttca actggaaaag cggcaagtca gaaaaatgta tcttctgtta
     5581 tccgcgaatt gagtcaggcc aacccaccgt gtgctcagaa acctgcgtgg gtcgcatccg
     5641 gtatctgggc gtgctgcttt acgacgccga ccgcattgag gaagcggcga gcaccgagcg
     5701 cgaagttgac ctctatgaac gtcagtgcga agtgttcctc gatccacacg atccctcagt
     5761 gatcgaggaa gccctgaaac aaggtattcc acaaaacgtg attgaagctg cccagcgttc
     5821 gcctgtctac aaaatggcga tggactggaa actggcgcta ccgctgcacc ctgaatatcg
     5881 caccctgcca atggtctggt acgttcctcc gctgtcgccg attcagtcct acgcagatgc
     5941 gggcggtttg ccgaaaagcg aaggcgtgct gcccgccatc gaaagcctgc gtattccggt
     6001 gcaatatctc gccaatatgt tgagtgccgg cgataccggt ccggtactgc gggcgctgaa
     6061 acggatgatg gcgatgcgcc actatatgcg ttcacaaacc gtggaaggcg ttactgatac
     6121 tcgtgccatc gacgaagtag gcctgagcgt cgcccaggtc gaagaaatgt atcgctatct
     6181 cgccattgcc aactatgaag atcgttttgt tatcccgacc agccatcggg aaatggcggg
     6241 cgatgccttc gcagaacgca acggctgcgg ttttaccttt ggcgacggtt gccacggttc
     6301 ggacagtaaa ttcaacctgt tcaacagtag ccgtatcgat gccatcaaca tcaccgaagt
     6361 gcgcgacaaa gcggagggcg aataatgcag atcctcaaag tgatcggcct gttgatggag
     6421 tatccggatg agctgttgtg ggaatgcaag gaggacgcgc tggcgttgat ccgccgcgac
     6481 gcgccgatgc ttacggattt cacccacaac ctgcttaacg cgccgctgct ggataaacag
     6541 gccgaatggt gcgaagtgtt tgaccgcggg cgcaccacgt cgctgctgct gttcgaacat
     6601 gttcatgccg agtcccgcga tcgcggccag gcaatggtgg acctgctggc ggagtatgaa
     6661 aaggtcggcc tgcagctgga ttgtcgggaa ctgcccgatt atctaccgct gtatctggag
     6721 tatttaagcg tactgcctga cgatcaggcg aaagaaggat tgctcaatgt tgcgccgatc
     6781 ctcgccctgc ttggcggtcg cttaaaacaa cgcgaggcac cgtggtacgc gttgtttgat
     6841 gctctgctgc aactggcggg aagtatactt tcaagtgaca gtgtcacaaa acaagtaaac
     6901 agcgaagagc gcgatgacac ccgccaggcg cttgatgcag tgtgggaaga ggaacaggtg
     6961 aagtttattg aagataacgc cacggcgtgt gacagctcgc cgcttaatca atatcagcga
     7021 cgctttagcc aggatgtcgc gccgcagtat gtcgacatca gtgcgggagg tgggaaatga
     7081 ttcagtatct gaacgtcttt ttttacgata tctacccgta catttgtgcg acggtgtttt
     7141 tcctcggcag ctggctgcgc tacgactacg ggcagtacac ctggcgcgcc tcctcaagtc
     7201 aaatgctcga taaacgcggg atggtgatat ggtcgaatct gttccatatc ggcattttgg
     7261 ggattttctt cgggcacttg ttcggcatgt taacgccgca ctggatgtac gcgtggtttt
     7321 tgccagtggc agcgaaacaa ctcatggcga tggtgctcgg cggtatttgc ggtgtgttga
     7381 cgttgattgg cggcgcagga ctgctgtggc gcagtctgac aaatcagcgc gtgcgtgcca
     7441 cttccactac gccggatatc atcatcatga gcattttgct gatccagtgt ctgttggggc
     7501 taagtaccat accgttttca gcacagtatc ctgatggtag cgaaatgatg aagctggtgg
     7561 gttgggcgca aagcattgtc actttccgtg gtggatcgtc agagatgctc aacggcgtag
     7621 cgttcgtctt ccgcctgcat ctggtgttgg gaatgaccat cttcctgctc ttcccgttca
     7681 cccgactggt gcatgtgtgg agcgcgccgt ttgagtactt tactcgtcga tatcaaattg
     7741 tgcgttcgcg gcgataattt caccctggcc ggatagccct tcgctctccg gccacttgca
     7801 ggctatgata gttctccagt cacaggagaa cgttaatgaa accgcaggtg taccacgtcg
     7861 atgcttttac ctctcaacca tttcgcggca attctgccgg tgttgttttc cccgccgata
     7921 atcttagcga agcacaaatg cagcttatcg cccgcgagtt aggccattcg gaaactgctt
     7981 ttctgctgca cagcgacgac agcgatgtgc gcatccgcta ctttacgcca acggttgaag
     8041 tgccgatttg cggtcacgcg acagtagctg cgcactatgt acgtgcgaag gtgttaggtt
     8101 taggaaattg cacgatctgg caaacatcgc tggcaggaaa acatcgcgtg actatcgaaa
     8161 agcacaacga tgattatcgt atttcgctgg aacaaggtac gccgggcttt gaactgccgc
     8221 tggagggtga agtacgcgcg gcgattatca acgcattgca tcttactgaa gacgatattc
     8281 tgccgggttt gccgatccag gtcgcgacga ccgggcattc taaagtgatg atcccattga
     8341 agccggaagt ggatatcgac gctctttcgc ctgaccttaa tgcgctgacc gctatcagta
     8401 aacagattgg ctgcaatggt ttcttcccgt tccagatccg tccaggcaaa aacgaaaccg
     8461 atggtcgtat gttctcgcct gcgattggta ttgtggaaga tccggtgact ggcaatgcca
     8521 atggcccgat gggtgcatgg ttggtacatc acaacgtatt gccccacgat ggcaacgtgt
     8581 tgtgtgttaa aggccatcag gggcgcgcat tggggcgtga cggcatgatt gaagtgacgg
     8641 tgacaattcg tgataaccaa ccggaaaaag tcaccatttc aggcacagcg gtgattttgt
     8701 ttcatgccga atgggcaatt gaaatctgaa atcgttgcct gatgcgcttc gcctgtcagg
     8761 tctacaaccg ggctaatgta tgccggataa ggcgtttacg ccgcatccgg caacctgaca
     8821 taaattattt tcccgcctcc ggatgcgtat caaacgccgc catcaccagc gctaactcat
     8881 ccacagtaaa gccatgtttc gcatcatcca cgcccagacc aaactgttcc tgcatcacgg
     8941 catataacga cgccacatcc ggtagatttc gctgctccac cgcgtgccca ttttcataat
     9001 gggtaaaatg aaaattggtc agtgtcagct tgccgccgtc cggcaaatgg cggcacatca
     9061 gcaaatgatg gcgaaaatgc gactgcggcc agtgcgccga ccagaaattg cccatcacat
     9121 aatcgctttg ttgctgctcg cagagatcga agcggtacat cggctgccaa tgctgatgat
     9181 gattaaactg caacacccaa tcatcaccct cctgcaacaa ccgatactct ccgtgtggcg
     9241 tggtctgcac gagatcggaa actaaacgaa tcggcgcggt tagcgtctgc ccaccgaaac
     9301 cgacatcagc aatccatttt tccccttcca gttccaccaa cagcaaacga tgggtgcgcg
     9361 gcggtaatgc tggcggattt gataacacta cgcgccctaa caagctgcga acgttaaacc
     9421 ccagctcgcg taacacccgc tcaaacacgc cattctgctc aaaacagtaa ccgccacgac
     9481 gggctatcac cagtttctct tccagcgatt gatcatcaag ctgtatttcc ctcggcagca
     9541 aaacgtcaag gttttcaaac ggaatggtgc aattgtgttt caggtgcaat gcacgaagcg
     9601 tatcaatatt gaccgcagca gctcccgacc agttaatacg ggcaaaatag tgattcagaa
     9661 tgggcgtcat caggcgtttc ccttagtggt tttctcaact tataacctgg aatagacgcc
     9721 gcaccggcta ttttgtgcga tcgcggacat gcatcacgtt tgcaaatcgc caatcccgtt
     9781 aatttcgtta cttattgcgt tacggactac gcttaaaaaa accttcttcg cgaggcgata
     9841 ccatgagccg attcatcccc atagaattac accatgccag ccgtctgtta aatcatggcc
     9901 caactgtctt gatcaccagt ttcgatgagc aatcccagcg gcgtaacatt atggctgcag
     9961 cctggtcaat gccggtggag tttgaacctc cacgcgtggc aattgtggta gataaatcga
    10021 catggaccag ggagttgatt gaacgtaacg gtaagtttgg catcgttatc ccgggcgttg
    10081 cagcaactaa ctggacgtgg gcggtgggaa gtgtgtcggt gcgtgaagaa gataaattta
    10141 attgctatgg cattccggtg gtgaaaggtc cggtgcttgg tttgccactt gttgaagaga
    10201 aatgcctggc gtggatggag tgccgcctgc tacctgcgac ttctgcgcaa caaaaatacg
    10261 acacgctgtt tggcgaggtg gtatcagcag cggcagacgc acgggtattt gtcgaaggcc
    10321 gctggcagtt tgatgatgat aagctcaata cgttgcatca tttaggtgct ggcacgtttg
    10381 ttactagcgg caagcgtgtt acggcaggtt aagcattaca tgctgtaacc gggtttcttt
    10441 atcaaagcct ccatttgggg ctcgatttcg gcatcccaga tagcttgcca agattctggt
    10501 tgaatctgct gtagagcaat gcttatcgag ctatctttac tgttcagatg gcgaataata
    10561 acgtcggtaa tatctgcagc aagtgctgct ttttgttgtt cgtccagttc acgcggaaag
    10621 catttaatgt cgatgtgcgg catgattacg ctccttttga aagatgtcag taaaattaac
    10681 acacattgtg aatgtcatgt gtccggtaaa gcgtttatac gacaataatg cccagggcaa
    10741 gattacgaaa gcccgctccc cgtaaggact gacgccaggt agtttctgcc catggctgct
    10801 tttcgcatct tacgtcttaa ccctgccttg aataccttat cattcgtcaa aa
//
LOCUS       DAJVRF010000132        10747 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJVRF010000132 DAJVRF010000000
VERSION     DAJVRF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10747
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.132"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            452..2380
                     /gene="thrS"
                     /locus_tag="OQN13_003197"
     CDS             452..2380
                     /gene="thrS"
                     /locus_tag="OQN13_003197"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCS1616479.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2384..2926
                     /gene="infC"
                     /locus_tag="OQN13_003198"
     CDS             2384..2926
                     /gene="infC"
                     /locus_tag="OQN13_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCS1616480.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3023..3220
                     /gene="rpmI"
                     /locus_tag="OQN13_003199"
     CDS             3023..3220
                     /gene="rpmI"
                     /locus_tag="OQN13_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCS1616481.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3273..3629
                     /gene="rplT"
                     /locus_tag="OQN13_003200"
     CDS             3273..3629
                     /gene="rplT"
                     /locus_tag="OQN13_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCS1616482.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    3746..3870
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3752..3796
                     /gene="pheM"
                     /locus_tag="OQN13_003201"
     CDS             3752..3796
                     /gene="pheM"
                     /locus_tag="OQN13_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCS1616483.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4079..5062
                     /gene="pheS"
                     /locus_tag="OQN13_003202"
     CDS             4079..5062
                     /gene="pheS"
                     /locus_tag="OQN13_003202"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCS1616484.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5077..7464
                     /gene="pheT"
                     /locus_tag="OQN13_003203"
     CDS             5077..7464
                     /gene="pheT"
                     /locus_tag="OQN13_003203"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCS1616485.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7469..7768
                     /gene="ihfA"
                     /locus_tag="OQN13_003204"
     CDS             7469..7768
                     /gene="ihfA"
                     /locus_tag="OQN13_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCS1616486.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7869..8849
                     /gene="btuC"
                     /locus_tag="OQN13_003205"
     CDS             7869..8849
                     /gene="btuC"
                     /locus_tag="OQN13_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCS1616487.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            8912..9463
                     /gene="btuE"
                     /locus_tag="OQN13_003206"
     CDS             8912..9463
                     /gene="btuE"
                     /locus_tag="OQN13_003206"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCS1616488.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPENPIVMESIKLALAK"
     gene            9463..10212
                     /gene="btuD"
                     /locus_tag="OQN13_003207"
     CDS             9463..10212
                     /gene="btuD"
                     /locus_tag="OQN13_003207"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCS1616489.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10290..>10747
                     /locus_tag="OQN13_003208"
     CDS             10290..>10747
                     /locus_tag="OQN13_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCS1616490.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         2692 a         2550 c         2911 g         2594 t
ORIGIN      
        1 acgaaaaatt gctataagaa ttgtgtcact tttatcattg tgatatgacc acttcgtcat
       61 taatctcatg agcgtttaaa atactaccgc caaatcacgc aacgaacttt gctggattca
      121 gtttatgctg ctgtaaatcc gctcgcagta aacctttcag acgcaccgtg atgttatcag
      181 tggttcttta agcgttttgc tggtgtactc actacaaacg aattgcgaat cgaatcaatg
      241 tgaaacggaa aggtacaatc tccctttttt tagtgttaac atcgttcaac cgggtggaag
      301 gtgagaggtt aatgtctttc atttgtgtac ctgattgcga accaatttag catttgttgg
      361 ctagatggtt tcgcaatgaa ctgttaataa acaaattttt ctttgtatgt gatctttcgt
      421 gtgggtcacc actgcaaatt aaggatataa aatgcctgtt ataactcttc ctgatggcag
      481 ccaacgccat tacgaccacg ctgtaagccc catggatgtt gcgctggaca ttggtccagg
      541 tctggcgaaa gcctgtatcg cagggcgcgt taatggcgaa ctggttgatg cttgcgatct
      601 gattgaaaac gacgcacaac tgtcgatcat taccgccaaa gacgaagaag gtctggagat
      661 cattcgtcac tcctgtgcgc acctgttagg gcatgcgatt aaacaacttt ggccgcatac
      721 caaaatggca atcggcccgg ttattgacaa cggtttttat tacgacgttg atcttgaccg
      781 cacgttaacc caggaagatg tcgaagcact cgagaagcgg atgcatgagc ttgctgagaa
      841 aaactacgac gtcattaaga agaaagtcag ctggcacgaa gcgcgtgaaa ctttcgccaa
      901 ccgtggggag agctacaaag tctccattct tgacgaaaac atcgcccatg atgacaagcc
      961 aggtctgtac ttccatgaag aatatgtcga tatgtgccgc ggtccgcacg taccgaacat
     1021 gcgtttctgc catcatttca aactaatgaa aacggcaggg gcttactggc gtggcgacag
     1081 caacaacaaa atgttgcaac gtatttacgg tacggcgtgg gcagacaaaa aagcacttaa
     1141 cgcttacctg cagcgcctgg aagaagccgc gaaacgcgac caccgtaaaa tcggtaaaca
     1201 gctcgacctg taccatatgc aggaagaagc gccgggtatg gtattctggc acaacgacgg
     1261 ctggaccatc ttccgtgaac tggaagtgtt tgttcgttct aaactgaaag agtaccagta
     1321 tcaggaagtt aaaggtccgt tcatgatgga ccgtgtcctg tgggaaaaaa ccggtcactg
     1381 ggacaactac aaagatgcaa tgttcaccac atcttctgag aaccgtgaat actgcattaa
     1441 gccgatgaac tgcccgggtc acgtacaaat tttcaaccag gggctgaagt cttatcgcga
     1501 tctgccgctg cgtatggccg agtttggtag ctgccaccgt aacgagccgt caggttcgct
     1561 gcatggcctg atgcgcgtgc gtggatttac ccaggatgac gcgcatatct tctgtactga
     1621 agaacaaatt cgcgatgaag ttaacggatg tatccgttta gtctatgata tgtacagcac
     1681 ttttggcttc gagaagatcg tcgtcaaact ctccactcgt cctgaaaaac gtattggcag
     1741 cgacgaaatg tgggatcgtg ctgaggcgga cctggcggtt gcgctggaag aaaacaacat
     1801 cccgtttgaa tatcaactgg gtgaaggcgc tttctacggt ccgaaaattg aatttaccct
     1861 gtatgactgc ctcgatcgtg catggcagtg cggtacagta cagctggact tctccttgcc
     1921 gtctcgtctg agcgcctcct atgtgggcga agacaacgag cgtaaggtac cggtaatgat
     1981 tcaccgcgca attctggggt cgatggaacg tttcatcggt atcctgaccg aagaattcgc
     2041 tggtttcttc ccgacctggc ttgcgccagt tcaggttgtt atcatgaata ttaccgattc
     2101 acagtctgaa tacgttaacg aattgacgca aaaactatca aatgcgggca ttcgtgttaa
     2161 agcagacttg agaaatgaga agattggctt taaaatccgc gagcacactt tgcgtcgcgt
     2221 cccatatatg ttggtctgtg gtgataaaga ggtggaatca ggcaaagttg ccgttcgcac
     2281 ccgccgtggt aaagacctgg gaagcatgga cgtaaatgaa gtgatcgaga agctgcaaca
     2341 agagattcgc agccgcagtc ttaaacaatt ggaggaataa ggtattaaag gcggaaaacg
     2401 agttcaaacg gcgcgcccta accgtatcaa tggcgaaatt cgcgcccagg aagttcgctt
     2461 aacaggtctg gaaggcgagc agcttggtat tgtgagtctg agagaagctc tggagaaagc
     2521 agaagaagcc ggagtagact tagtcgagat cagccctaac gccgagccgc cggtttgtcg
     2581 tataatggat tacggcaaat tcctctatga aaagagcaag tcttctaagg aacagaagaa
     2641 aaagcaaaaa gttatccagg ttaaggaaat taaattccgt cctggtacag atgaaggcga
     2701 ctatcaggta aaactccgca gcctgattcg ctttctcgaa gagggtgata aagccaaaat
     2761 cacgctgcgt ttccgcggtc gtgagatggc gcaccagcaa atcggtatgg aagtgcttaa
     2821 tcgcgtgaaa gacgatttgc aagaactggc agtggtcgaa tccttcccaa cgaagatcga
     2881 aggccgccag atgatcatgg tgctcgctcc taagaagaaa cagtaaggcc ttcaagtagc
     2941 actgtctgtg gagccttcgg gcttcacagg ctttgttcgc ctttgtttcg tttattaaca
     3001 atgcgaagtg gaagttatta aaatgccaaa aattaagacc gtacgcggtg ctgctaagcg
     3061 cttcaaaaaa accggtaaag gtggttttaa gcacaagcac gctaacctgc gtcacattct
     3121 gaccaaaaaa gcgaccaaac gtaaacgtca cctgcgtccg aaagccatgg tttccaaagg
     3181 cgatctgggc ctggtaatcg cgtgcctgcc gtacgcataa gccgttaacg tttttaactt
     3241 tttaattaga atatagatac aggagagcac atatggctcg cgtaaaacgt ggtgttattg
     3301 cacgcgcacg tcacaagaaa attttgaaac aagctaaagg ctactacggt gcgcgttctc
     3361 gcgtataccg cgttgccttc caggctgtta tcaaagctgg tcagtatgct taccgtgacc
     3421 gtcgtcaacg taagcgtcag ttccgtcaac tgtggattgc gcgtatcaac gcagcagcac
     3481 gtcagaacgg tatttcttac agcaaattca tcaatggcct gaaaaaagcc tctgttgaaa
     3541 tcgaccgtaa gatcctggct gatatcgcag tattcgacaa agtagcgttc accgctctgg
     3601 ttgaaaaagc gaaagcagct ctggcataag ccagttgaaa gagggagcta gtctccctct
     3661 tttcgtttca acgccatcaa aacattgact tttatcgccg tagccttttc aataaaggtc
     3721 ttttgaagag taccaaaagg taacgcaagc aatgaatgct gctattttcc gcttcttttt
     3781 ttactttagc acctgaatcc aggaggctag cgcgtgagaa gagaaacgga aaacagcgcc
     3841 tgaaagcctc ccagtggagg ctttttttgt atgcgcgttt tgaaaaataa aaagccagtc
     3901 tatcaggcgt aattgtcgca gccagtctgg acacggccag cgcggagaaa ccggagcgta
     3961 tttaagtacg tgagaatttc gagcactgcc cgggaccaaa atggcaagta aaatagcctg
     4021 atgggatagg ctctaagtcc aacgaaccag tgtcaccact gacacaatga ggaaaaccat
     4081 gtcacatctc gcagaactgg ttgccagtgc gaaggcggcc attagccagg cgtcagatgt
     4141 tgccgcgtta gataatgtgc gcgtcgaata tttgggtaaa aaagggcact taacccttca
     4201 gatgacgacc ctgcgtgagc tgccgccaga agagcgtccg gcagctggtg cggttatcaa
     4261 cgaagcgaaa gagcaggttc agcaggcgct gaatgcgcgt aaagcggaac tggaaagcgc
     4321 agcgctgaat gcgcgtctgg cggcggaaac gattgatgtc tccctgctag gtcgtcgcat
     4381 tgaaaacggc ggtctgcatc cggttacccg taccatcgac cgtatcgaaa gtttcttcgg
     4441 tgagcttggc tttaccgtgg caaccgggcc ggaaatcgaa gacgattatc ataacttcga
     4501 tgctctgaac attcctggtc accacccggc gcgcgctgac cacgacactt tctggtttga
     4561 cactacccgc ctgctgcgta cccagacctc tggcgtacag atccgcacca tgaaagccca
     4621 gcagccaccg attcgtatca tcgcgcctgg ccgcgtttat cgtaacgact acgaccagac
     4681 tcacacgccg atgttccatc agatggaagg tctgattgtt gataccaaca tcagctttac
     4741 caacctgaaa ggcactctgc acgatttcct gcgtaacttc tttgaggaag atttgcagat
     4801 tcgcttccgt ccttcctact tcccgttcac cgagccttct gcagaagttg acgtcatggg
     4861 taaaaacggt aaatggctgg aagtgctggg ttgcgggatg gtgcatccga acgtgctgcg
     4921 taacgttggc atcgacccgg aagtttactc tggtttcgcc ttcgggatgg ggatggagcg
     4981 tctgactatg ttgcgttacg gcgtcaccga cctgcgttca ttcttcgaaa acgatctgcg
     5041 tttcctcaaa cagtttaaat aaggcaggaa tagattatga aattcagtga actgtggtta
     5101 cgcgaatggg tgaacccggc gattgatagc gatgcgctgg cgaatcaaat cactatggcg
     5161 ggcctggaag ttgacggtgt agaaccggtt gccggtagct tccacggcgt ggtcgttggt
     5221 gaagtggttg agtgtgcgca gcatccgaac gctgacaaac tgcgtgtgac aaaagtgaat
     5281 gtcggcggcg atcgcctgct ggacatcgtc tgtggtgcgc caaactgccg tcagggcctg
     5341 cgtgtggcgg tagctaccat tggtgctgtt ctgccgggtg atttcaaaat taaagcggcg
     5401 aaactgcgtg gcgaaccgtc tgaagggatg ctgtgctcct tctctgaact gggaatttct
     5461 gacgatcaca gcggcattat cgaactgcct gcagatgcgc cgattggcac tgacatccgc
     5521 gaatacctga aactcgatga caacaccatc gaaatcagcg taacgccaaa ccgtgccgac
     5581 tgcttaggta tcattggtgt tgcgcgtgac gttgccgtgc tgaatcagct gccgctggtt
     5641 gaaccggaaa tcgttccggt tggtgcgacc atcgacgaca cgttgccgat tacagtcgaa
     5701 gcgcctgaag cctgcccgcg ctatcttggc cgtgtggtaa aaggcattaa cgttaaagcg
     5761 ccaactccgc tgtggatgaa agaaaaactg cgtcgttgcg ggatccgttc tatcgatgca
     5821 gttgttgacg tcaccaacta tgtactgctc gaactgggcc agccgatgca cgctttcgat
     5881 aaagatcgca ttgaaggcgg cattgtggtg cggatggcaa aagagggcga aacgctggta
     5941 ctgcttgatg gcactgaagc gaagctgaat gctgacactc tggtcatcgc cgaccacaac
     6001 aaggcgctgg cgatgggcgg catctttggt ggcgaacact ctggcgtgaa tgacgaaacg
     6061 caaaacgtct tgctggaatg cgctttcttc agcccgctgt ctatcaccgg tcgtgctcgt
     6121 cgtcatggcc tgcatactga tgcgtctcac cgttatgagc gtggcgttga tccggcactg
     6181 cagcacaaag cgatggaacg tgcgacccgt ctgctgatcg acatctgcgg tggtgaggct
     6241 ggtccggtaa ttgatatcac caacgaagca acgctgccga agcgtgcaac catcacttta
     6301 cgtcgtagca aactggatcg cctgatcggc catcatattg ctgatgaaca ggtaactgac
     6361 attctgcgtc gtctcggctg cgaagtgacc gaaggcaaag acgagtggca ggcagttgcg
     6421 ccgagctggc gtttcgatat ggagattgaa gaagatctgg ttgaagaagt cgcgcgtgtt
     6481 tacggctaca acaacatccc ggatgagccg gtacaggcaa gcctgattat gggtactcac
     6541 cgtgaagctg acctgtcgct caagcgcgtg aaaacgctgc tcaacgacaa aggctatcag
     6601 gaagtgatca cctacagctt cgttgatccg aaagtgcagc agatgatcca tccaggcgtt
     6661 gaagccttac tgctgccaag cccgatctct gttgaaatgt cagcaatgcg tctttctctg
     6721 tggaccggcc tgctggcaac cgtggtgtac aaccagaacc gtcagcagaa ccgtgtgcgc
     6781 attttcgaaa gcggtctgcg tttcgtacca gatactcagg caccgttggg cattcgtcag
     6841 gatctgatgt tagccggtgt gatttgcggt aaccgttacg aagagcactg gaacctggca
     6901 aaagagaccg ttgatttcta tgatttgaaa ggcgatcttg aatccgttct cgacctgacc
     6961 ggtaaactga atgaggttga gttccgtgca gaagcgaatc cggcactgca tccggggcaa
     7021 tccgcagcga tttatctgaa aggggaacgt attggttttg ttggagttgt tcatcctgaa
     7081 ctggaacgta aactggatct taacggtcgc actctggtgt tcgaactgga gtggaacaag
     7141 ctcgcagacc gcgtggtgcc tcaggcgcgc gagatttctc gcttcccggc gaaccgtcgt
     7201 gacatcgcgg tggtggtcgc agaaaacgtt cccgcagcgg atattttatc cgaatgtaag
     7261 aaagttggcg taaatcaggt agttggcgta aacttatttg acgtgtaccg cggtaagggt
     7321 gttgcggagg ggtataagag cctcgccata agcctgatcc tgcaagatac cagccgtaca
     7381 ctcgaagaag aggagattgc cgctaccgtc gccaaatgtg tagaggcatt aaaagagcga
     7441 ttccaggcat cattgaggga ttgaacctat ggcgcttaca aaagctgaaa tgtcagaata
     7501 tctgtttgat aagcttgggc ttagcaagcg ggatgccaaa gaactggttg aactgttttt
     7561 cgaagagatc cgtcgcgctc tggaaaacgg cgaacaggtg aaactctctg gttttggtaa
     7621 cttcgatctg cgtgataaga atcaacgccc gggacgtaac ccgaaaacgg gcgaggatat
     7681 tcccattaca gcacggcgcg tggtgacctt cagacccggg cagaagttaa aaagccgggt
     7741 cgaaaacgct tcgcccaaag acgagtaatc tgatctaact aaaaaggccg ctctgcggcc
     7801 ttttttcttt tcactgtcga agagtcaccg taaaatcaac gccatgacac ttcagcagaa
     7861 cggataccat gctgacactt gcccgccaac aacagcgaca aaatattcgc tggttattat
     7921 gcctgtcagt tttgatgctg ctggcgcttc tcttaagcct ttgcgccggt gaacaatgga
     7981 tttcgccagg tgactggttt actcctcgtg gcgaactgtt cgtctggcaa attcgcctgc
     8041 cacgtacgct ggctgtattg ctggttggtg cggcgctggc tatatccggc gctgtaatgc
     8101 aggcgttgtt tgaaaatcct ctggcagaac ctggactact tggcgtctct aacggcgcag
     8161 gcgtggggct tatcgccgcg gtattgcttg ggcaagggca actccccaac tgggcgctag
     8221 ggctgtgtgc gattgctggc gcgcttatca tcactttaat actcttacgt ttcgcccgtc
     8281 gtcatctttc gaccagtcgg ttattgctgg ctggcgttgc attagggatt atctgtagcg
     8341 cactaatgac gtgggctatc tacttttcca cctctgttga tttacgtcag ctgatgtact
     8401 ggatgatggg cggttttggc ggcgtagact ggcggcaaag ctggctgatg ctggcattga
     8461 tccccgtgtt gttgtggatc tgttgtcagt ccaggccgat gaatatgtta gcacttggcg
     8521 agatctcggc gcggcaactg ggtttacccc tgtggttctg gcgcaatgtg ctggtggcag
     8581 cgaccggctg gatggttggc gtcagtgtgg cgctggcggg tgctatcggc tttattggtc
     8641 tggtgatccc acatattctt cggttgtgtg gtttaaccga tcatcgcgta ttacttcccg
     8701 gctgcgcgct ggcaggggcg agcgcagtgc tgctggccga tattgtagcg cgcctggcat
     8761 tagctgccgc agagctgcct attggcgtgg tcaccgcaac gttgggtgcg ccggtgttta
     8821 tctggttatt gttaaaagca ggacgttagc cgcaaaaaga cggtctatga ttaaaagcta
     8881 agatttttac tgaccacacc caggagaaac gatgcaagat tccattctga cgaccgtagt
     8941 gaaagatatc gacggtgaag tgaccacgct ggagaagtac gccggtaatg tgctgttgat
     9001 tgtcaatgtc gcctcaaagt gtggcttaac gccgcaatat gagcagttgg agaatattca
     9061 gaaagcctgg gccgatcgag gttttgtggt gctgggattc ccgtgcaacc agtttctgga
     9121 acaagaaccg ggcagcgatg aagagattaa aacttactgt accaccacat ggggggtgac
     9181 gttcccgatg ttcagtaaga ttgaagttaa cggcgaagga cgccatccgc tgtatcaaaa
     9241 attgattgcc gcagcgccga ccgcagtcgc gccggaagag agcggattct atgcccgtat
     9301 ggtcagcaaa ggtcgtgcac cgctgtaccc tgatgatatt ttatggaatt ttgagaaatt
     9361 tttagttggc agggacggat tggtcatcca gcgtttttcc ccggatatga cgccggaaaa
     9421 ccccattgtg atggaaagca ttaaactggc gttggcaaaa taatgtctat tgtgatgcag
     9481 ttacaggatg ttgcggaatc tacccgccta gggccacttt ctggcgaggt tcgggctggg
     9541 gagatcctgc acctggtggg gccgaatggc gcgggtaaga gtaccttact ggcacgaatg
     9601 gccggaatga ccagcggtaa gggaagtatt cagttcgcgg ggcaaccact ggaagcatgg
     9661 tccgcaacaa aactcgcgct gcatcgcgcc tatctttcac aacagcagac gccgccgttt
     9721 gcaatgccgg tctggcacta tctgacactg catcagcacg ataaaacgcg taccgaacta
     9781 ctgaatgatg tcgcaggggc gctggctctt gatgacaaac tcggacgtag caccaatcaa
     9841 ctttccggcg gtgaatggca acgcgtacgt cttgctgcgg tggtgctgca aatcacacca
     9901 caagccaatc ccgcaggcca attgctgctt cttgatgagc cgatgaacag tcttgatgtt
     9961 gcgcaacaaa gtgcgttaga caaaattctg agcgctctgt gtcagcaagg actggcgatt
    10021 gtgatgagca gtcacgatct caaccacaca ttgcgtcatg cgcatcgggc gtggttgcta
    10081 aaaggtggaa aaatgctggc cagtggacgc agagaagagg tgctcacgcc gccaaatctg
    10141 gcgcaggcct atgggatgaa ttttcgccgt ctggatatcg aaggtcacag aatgctgatt
    10201 tcgaccatct gatttcatta gtatcttgaa aatcgggtaa ttacgacgct aaattaatgc
    10261 aagaataaaa aacagaggaa ccgtacggaa tgcgtttctg ccttattttg atcacagcac
    10321 tgtttctggc cgggtgtagc caccataaag caccgccgcc aaatgccaga ctttctgatt
    10381 cgattaccgt tattgccggt ttgaacgacc agctacaaag ctggcatggc acgccgtatc
    10441 gttatggtgg catgacgcgg cgcggtgtgg actgttcggg atttgtggtt gtaacgatgc
    10501 gcgatcgttt cgatttgcag ctgccccgag aaaccaaaga acaagcctct atcggcacgc
    10561 aaattgataa agacgagttg ctgcctggtg acctggtctt tttcaaaacg ggttccggac
    10621 aaaatggttt gcatgtaggt atttacgata ccaacaacca atttatccac gcctctacca
    10681 gcaagggagt gatgcgttcc tcacttgata atgtctattg gcagaaaaat ttctggcagg
    10741 cgagacg
//
LOCUS       DAJVRF010000133        10719 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJVRF010000133 DAJVRF010000000
VERSION     DAJVRF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10719
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.133"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            1..1094
                     /gene="dacC"
                     /locus_tag="OQN13_003209"
                     /pseudo
     CDS             1..1094
                     /gene="dacC"
                     /locus_tag="OQN13_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            complement(1141..1899)
                     /gene="deoR"
                     /locus_tag="OQN13_003210"
     CDS             complement(1141..1899)
                     /gene="deoR"
                     /locus_tag="OQN13_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCS1616491.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1957..2553)
                     /gene="ybjG"
                     /locus_tag="OQN13_003211"
     CDS             complement(1957..2553)
                     /gene="ybjG"
                     /locus_tag="OQN13_003211"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS1616492.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            2838..4065
                     /gene="mdfA"
                     /locus_tag="OQN13_003212"
                     /pseudo
     CDS             2838..4065
                     /gene="mdfA"
                     /locus_tag="OQN13_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(4106..4390)
                     /locus_tag="OQN13_003213"
     CDS             complement(4106..4390)
                     /locus_tag="OQN13_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616493.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(4476..5291)
                     /gene="ybjI"
                     /locus_tag="OQN13_003214"
     CDS             complement(4476..5291)
                     /gene="ybjI"
                     /locus_tag="OQN13_003214"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCS1616494.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(5291..6499)
                     /locus_tag="OQN13_003215"
     CDS             complement(5291..6499)
                     /locus_tag="OQN13_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1616495.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            6583..7119
                     /gene="rcdA"
                     /locus_tag="OQN13_003216"
     CDS             6583..7119
                     /gene="rcdA"
                     /locus_tag="OQN13_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCS1616496.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(7294..8979)
                     /locus_tag="OQN13_003217"
     CDS             complement(7294..8979)
                     /locus_tag="OQN13_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCS1616497.1"
                     /translation="MNINVAELLNGNYILLLFVVLTLGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            9249..9626
                     /locus_tag="OQN13_003218"
     CDS             9249..9626
                     /locus_tag="OQN13_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCS1616498.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(9656..9913)
                     /gene="grxA"
                     /locus_tag="OQN13_003219"
     CDS             complement(9656..9913)
                     /gene="grxA"
                     /locus_tag="OQN13_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCS1616499.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            10073..10360
                     /locus_tag="OQN13_003220"
     CDS             10073..10360
                     /locus_tag="OQN13_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCS1616500.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            10344..>10719
                     /gene="nfsA"
                     /locus_tag="OQN13_003221"
     CDS             10344..>10719
                     /gene="nfsA"
                     /locus_tag="OQN13_003221"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCS1616501.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLG"
BASE COUNT         2765 a         2650 c         2716 g         2588 t
ORIGIN      
        1 ggatgcgcgt gcatggattt taatggatta cgccagcggt aaagtgctgg cagaaggcaa
       61 cgcggatgag aaactggatc ccgcgagcct gactaaaatc atgaccagct atgtggttgg
      121 gcaggcgctt aaggccgata agattaaact caccgatatg gtgacggtcg gtaaagatgc
      181 ctgggcgacg ggaaatccgg cactgcgtgg ttcatcggta atgttcctca aaccgggcga
      241 tcaggtttcg gtggcagact tgaacaaagg tgtgattatc cagtccggta atgacgcctg
      301 tattgcgctg gccgattacg ttgccgggag ccaggagtca tttattggtt tgatgaatgg
      361 ttatgccaaa aaaactgggt ctgaccaaca ctaccttcca gacggtgcac ggtctggatg
      421 cgccggggca gttcagtacc gcgcgcgata tggcattgct gggtaaagca ttgatccacg
      481 atgtgccgga agagtacgcc attcataaag agaaagagtt caccttcaac aaaattcgtc
      541 agcctaaccg taaccgtctg ctgtggagca gcaatctgaa tgttgatggc atgaagacag
      601 gaaccacggc aggtgcggga tataatctgg ttgcttcggc tacccagggt gatatgcgtt
      661 taatctccgt agtgctgggg gcgaaaactg accgtatccg ttttaatgag tctgagaaat
      721 tattgacctg gggtttccgc ttctttgaaa ctgtgacgcc aattaaacct gatgccacct
      781 ttgtgactca gcgcgtctgg tttggtgata agagcgaagt gaatctcggg gcaggcgaag
      841 cgggctccgt gaccataccg cgtgggcagc tgaaaaacct gaaggcgagc tacacgttaa
      901 cggaaccgca gcttaccgca ccgctgaaaa aaggtcaggt tgtcgggacc attgatttcc
      961 agcttaacgg taaatccatt gagcagcgtc cgctgatcgt gatggaaaat gtggaagagg
     1021 gcggattctt tggtcggatg tgggatttcg tgatgatgaa attccatcag tggttcggca
     1081 gctggttctc ttaatcttct gataaccgga tggcgcgaaa cgtcatccgg ttatacgtca
     1141 ttaatacatc aacttaatac ggtgcgtctg cgcgtacttc acatactcat cttccgggca
     1201 acaatcactc accacaatat caaagcgttt caggtcaccc atgcgcgccg gacgcacctt
     1261 gccaaattta ctgtggtcga caaccagcac atgcttttgc gccatcgaca tggcccagtg
     1321 ttttaccggc aactcttcaa gattaaaaca ggtagcgcct ttactgacat gcacgcccgc
     1381 cgcggaataa aaagcgatat ccgggcaaaa attattcaac gtttgctgaa aatcgatggg
     1441 gttgaaaatg gcgttgctgg cgtgaaattc accaccgcac agaaatgcgc ggcaatgggg
     1501 tttctctttc agcgccagaa aggtatttag cgaataacaa acggcggtaa aagggatttc
     1561 attatcaatc gcttcaataa tccacggcgt ggtggtgcca cagtcaaaaa agagggtctg
     1621 atcgggttct accagtgtcg cagccagttt tgccgcccgg cgtttttctt ccaccaggcg
     1681 ggatttttga tcgcttaaca ggtaatggct ggcactgcgc ggttccagaa caatatagcc
     1741 gccgagcaaa acgacgggcg cactgtggtt gttcagatcg cgacgaatcg tcatctccga
     1801 aaccccaagc agggcggcgg cgtctttaag atgtaactta tcgctgcgtt ttaattcttg
     1861 cagcagctgc ccgatacgct cttcgcgacg tgtttccata atccctctga atagttattg
     1921 aagcgagccg ctcaatacta cactttttag cagagatcag tcacgcaccc agcctttgcg
     1981 gatcggtaat gcaaaacaga cgcgatacca cgattgcaga cgttgataga gtttatgccc
     2041 catcgcttgc cagataatct gggcactaag gcagccaatc atacctgcca gcaatccacc
     2101 gagcatatcc agcggccagt ggacgccaag ataaacgcgc gaccaggcaa tgacgacggc
     2161 cagaaccatt aaaagtgagc cggaccacag gcgatgccag cataaaaatg ccagtgcaaa
     2221 ggtgaaaatc accgtaccgt gatcgctcgg gaatgagtca tccgccgcat gatgcaggaa
     2281 gttatagccg atattttcga caaagggtcg gtcgtgcgga aaaagatgtc ccatcgtcca
     2341 ggacacaaac aggctgaccg ccagcgcgat agcgattttt atcaccagtt gccgttgtgc
     2401 tgtaagcccc cacaaccaaa gtaccgcggc cagcaacggc accacggtaa tcaaatcttt
     2461 agcaataaaa atcgccaacg agatcatcca cggagccgag tctggcgtcg cgttaataag
     2521 agagaataga gagagattca aattttccag cataacttcc cgacgcaaag tgattaaaag
     2581 ggagccaata caggcaagtc gttgagaata aagtgcaggt taaactgggt aaagcggcat
     2641 cgtcttattt ccctcaagcg gcctgtttac ggtgggtgat tgtaacgggc ataggttaaa
     2701 taaaacttaa agaaagcgta gctatactcg caataatgta agaatgtgct taaccgtggt
     2761 ttcagctaca aaattcgctt tctcgttagc tgcgctttta ttaaactctg cgcgattatt
     2821 attggcgaag aaattgcatg caaaataaat tagctttcgg tgccaggctt ggacgtcagg
     2881 cgttactttt ccctctctgt ctggtgcttt acgaattttc aacctatatc ggcaacgata
     2941 tgatccaacc cggtatgttg gccgtggtgg aacaatatca ggcgggcatt gattgggttc
     3001 ctacttcgat gaccgcgtat ctggcgggcg ggatgttttt acaatggctc ctggggccgc
     3061 tgtcggatcg tattggtcgc cgtccggtga tgctggcggg tgtggtgtgg tttatcgtca
     3121 cctgtctggc aatattactg gcgcaaaaca ttgaacaatt caccctgttg cgcttcttgc
     3181 agggcataag cctctgtttc attggcgctg tgggatacgc cgcaattcag gaatccttcg
     3241 aagaggcggt ttgtatcaag atcaccgcgc tgatggcgaa cgtggcgctg attgctccgc
     3301 tacttggtcc gctggtgggc gcggcgtgga tccatgtgct gccctgggag gggatgtttg
     3361 ttttgtttgc cgcattggca gcgatctcct ttttcggtct gcaacgagct atgcctgaaa
     3421 ccgccacgcg tataggcgag aaactgtcac tgaaagaact cggtcgtgac tataagctgg
     3481 tgctgaagaa cggccgcttt gtggcggggg cgctggcgct gggattcgtt agcctgccat
     3541 tgctggcgtg gatcgcccag tcgccgatta tcatcattac cggggagcag ttgagcagct
     3601 atgaatatgg cttgctgcaa gtgcctattt tcggggcgtt aattgcgggt aacttgctgt
     3661 tagcgcgtct gacctcgcgc cgcaccgtac gtgataatta tgggcggctg gccgattatg
     3721 attggtctgt tggtcgctgc tgcggcaacg gttatctcat cgcacgcgta tttatggatg
     3781 actgccgggt taagtattta tgctttcggt attggtctgg cgaatgcggg actggtgcga
     3841 ttaaccctgt ttgccagcga tatgagtaaa ggtacggttt ctgcggcgat gggaatgctg
     3901 caaatgctga tctttaccgt tggtattgaa atcagcaaac atgcctggct gaacgggggc
     3961 aacggacagt ttaatctctt caaccttgtc aacggaattt tgtggctgtc gctgatggtt
     4021 atctttttaa aagataaaca gatgggaaat tctcacgaag ggtaaaaaaa tgcctgactg
     4081 ctttgtgcga tcaggcattc tcgaattaat ggtgatggtc gtcaatctgg tgttcgataa
     4141 ccatcccttc acctacgctg gcaagatggc gagcataagg atgcgggcgg taagccggag
     4201 ctggcgcagg agccacatag acggtttgcg gcacagtcgc gacgctgacc gcttgtggaa
     4261 cgctgactga atcaggaact acaaccactt tgtatccact cggcacatcg acggtgacac
     4321 tttgcgccat cgccacacca gtaaagccca ttaaaagcgc gcccaacagt agacaatttt
     4381 tcataatcat ttcgcctgaa ataatgcagc gggttaattt ccgcgaatta tgcagccttc
     4441 attcaggtat tgtgttgccg ggcgtgctgc cgtttttatt ggtcaaatgg cgcttcgtgt
     4501 ttaagcactt tatcgatcac atccagtacg ccttcacggt tattggagcc tgcccggtat
     4561 tttgccgctg cgacgaccgc gctgccggca ttttccattg caaaactaaa gcctgcctga
     4621 cgcagcatct caatatcgtt accgccatcg ccaaagacca ccacttcgct gtcgtctatt
     4681 ccccataatt tctgcagttg gcgaaggcca ttggctttat gtacgccggg gataatcaga
     4741 tcgatgctgc cgttgccggt gtggactggc accataatat cgccgatggc ctcatgtaat
     4801 gctttttgta cttgtggaat cagttcatcg gaaagattca ggccaaactt aaagaaaata
     4861 tcttctaagt tgtcaaagtt atcgacgtat tccagacggt gataatacat ttctgccacc
     4921 gttttcatgg catcgtcata ttttttgagc gtataggcgc tattttttcc gcaggcaata
     4981 atttccactt ccgggcgcgt cagcaaatgt tccacgacag tagtaaaggc atccttcgac
     5041 agctcgccat taaaaacatc tttgccttca ctcactaccc agccgccgtt ttcagccaca
     5101 aaggcaattt cattagcaat ttcagggaag aaagagagca actgataata ttgattcccg
     5161 ctggcgacca caaagcgaat gccttgtgct ttcatttgct gatactgagc cataaaccgc
     5221 tcacggttat aggttttttg atcgcttaag aaagtaccat ccatgtctac cgcaattaat
     5281 ttaatgctca tcaactattc tccatcgccg tccgcgtttt ggtatcgggt ttggcgacgg
     5341 ctttcgcgac aatagccgcg agaataacca gcgccagtac aaccagcatt gcactacgta
     5401 atccataatg ttcgccgaga tagcccagca gcggcggccc gacgaggaaa gccagataac
     5461 cggtcgttgc taccacactg acgcgggttg gtgcatcggg gccggtatcg ctggcggcag
     5521 aaatggtcag cgggaagccc agcgaggcac ccagtcccca gagtacaaca gacaccccag
     5581 cgacccaggc gctatcgaca aaaataatca gcccaatacc caacgccccc attagtgcac
     5641 tggcccgaac cacggcaacg cgactgtaac ggtcgatgaa ccaaccgcca gtgaagcgtc
     5701 caacggtcat ccctagggta aaaccggcat aaatcagcga gccggaagta gggctaaaac
     5761 cgtgaccgtc aaccattaat aagggtaacc agtcgttggc agaaccttcg gcaaaggcca
     5821 tcgccagcac tacaacacca atcaacagca actggatatc gcgataaaaa ggaatgcctt
     5881 tttcgccatg ctgggtgcca tcggcagcat ttttgcccgt accgtcaggg attgcctgaa
     5941 tggcgatata aataggcgcg atacctacca gcgccgccaa taaaatgtgc accgttgccg
     6001 gaacgccaaa ggccgtcagt gccatcccga caccagcgcc tgccagcgtg cctaggctat
     6061 aaaaaccgtg catcatcggc aaaaccgttt tattcatttc tcgctcaacg gcggcacctt
     6121 caacgtttat cgccacttcc gcagaaccaa aacttgcccc aaagacgccg agaccaacgg
     6181 caaagagcag gggcgatgtc agccagagtg ccagacttaa tatcatcatc ccgatcaatg
     6241 cgcaggacat cgtgaccagg atgacattac gtgtcccaaa gcgtttcact aaccacgccg
     6301 agcagagaat accgctcatc gaaccgatcg acagaccaaa gagaacaccg cccatttcag
     6361 cgatcgagac agagagaata tcgcggatag caggcgtacg ggttgcccag gacgccatta
     6421 acaggcctgg caaaaagaag aacataaaca gcgcccaggt tcggcgtttc aatgcattac
     6481 gtgaagaatt tacggtcata gatcacgtca aaataagaag agggaagaca acattagcaa
     6541 ggttgtgtac atttgtacac aattgcagac agaggaaatg acatgcgtcg cgctaacgat
     6601 ccgcaacggc gagaaaaaat tatccaggcc acgctggagg cggtgaaact ttacggaata
     6661 catgctgtta cgcaccgcaa aattgctacc cttgccgggg taccgttggg atcgatgacc
     6721 tactattttt caggaattga tgagttgttg ctggaggcgt tcagcagttt tactgagatt
     6781 atgtcccggc aatatcaggc attttttagc gatgttagtg atgctcaagg cgcatgccag
     6841 gctatcaccg atatgatcta cagctcacag gttgcaacgc cggataatat ggagctgatg
     6901 taccagctct acgcgctggc tagccgaaaa ccgctattaa aaacggtaat gcaaaactgg
     6961 atgcagcgca gtcagcaaac gctcgaacaa tggtttgaac ccggaaccgc ccgcgcgctt
     7021 gatgcgttta ttgaggggat gacgctgcat tttgtcaccg accgtaagcc gctatcgcgc
     7081 gaggagattt tgaggatggt tgagagggtt gcagggtagt agataaattt tagataacaa
     7141 aaaacccatc aaccttgaac cgaaatggcg gggttgatgg gctccacaaa atggggacat
     7201 caaagaaaag cagtggcact aattaagact gatgccctgc ggaaaagttc tgcggttgtg
     7261 caaaaaaatt tcattttcag ggcaacttca gttttatcct aatcctggcc ataccatgac
     7321 gatgattgtc cctgccagcg tcagcaggac gttggcgatt gcgtaggtgc ccgcatagcc
     7381 cagcgcaggg atgttactgc gagctgtatc actgatgatc tccattgccg gcgcgcaggt
     7441 gcgtgcgccc atcattgcgc cgaacaacag tgcgcggttc attcgcaata cataagcacc
     7501 gaacaagaaa cagataacca cgggcacaag actgacaatt aatccggcaa tcaacatctg
     7561 accgccaatc gcgcccaggc cgttattaat accgctaccg gcgctcagac caacgcctgc
     7621 cataaacacc atcaagccga actctttcac catgcttaat gcaccttgcg gaatgtaacc
     7681 gaaggtcggg tggttagcac gcataaagcc cagcataatt ccggcgaata acaacccggc
     7741 agcgttcccc atgccgaaac tgaatgtgct gaactggaag gtgatcatcc cgatcatcag
     7801 cccaataaca aagaaggcgc aaaatgccag caggtcagtg acctggctgt gaatcgagat
     7861 aaagccgatg cgatcggcga tggtttttac gcgacgggca tcaccgctga cttgtaaaac
     7921 gtcacctttg ttaagcacga cgttgtcatc tatcggcatc tcaatctggc tacgaatgac
     7981 gcggttaagg aagcaaccgt gatcggtcaa cttcagttgt gcgagacgtt tacctacagc
     8041 gttatggttt ttaacgacca cttcttcagt gacgatacgc atgtcgagaa ggtcacgatc
     8101 gaaaacttct ttaccgttac ggaagctggg atcgagtcgg gcatgggcgt cgggatagcc
     8161 taccaacgct atttcatcgc ccatttgtag cacggcatca ccgtctggat ttgccagaat
     8221 cccgttacgt cgaatacgtt caatgtagca gccggtttgt cgataaatac ccagttcacg
     8281 cagatttttg ccgtcggtcc aggccaccag ctccgggccg acgcgatagg cgcggatcac
     8341 cggtaaataa accttacggt tggcatcagt gtccaggcca cgttcgcggg cgatttgctg
     8401 ggcgctggtc tgtaagtcct gatgctgcaa tttcggcaag taacgcgcac caacaatcaa
     8461 actcaccaga ccgattaaat aggttaaggc atacccgagg ctcagattat ccagtgccag
     8521 tgagagctgc ctgctttcca tgccggaatg acgcagtgta tcgccagcac cgaccagaac
     8581 cggtgtcgac gtcatagagc ctgctaacat accggccgtc aggccaatat cccagccaaa
     8641 cagcttacct aaccctaagg cgatcaccag cgcactgcca accatcacca gtgctaacat
     8701 taggtaattt ttcccatcgc gaaaaaaaat ggaaaaaaag ttcggtccgg cttcgacccc
     8761 gacgcagaaa ataaacagca taaagccaag attaagcgca tcggtgttaa tgctgaaatg
     8821 ttgttggcct aataacagcg atacgactaa aacgccaatg gaattaccca gttggatcga
     8881 accaagtcgt aactttccga gacatagccc aagcgtgagg accacaaata ataacagaat
     8941 gtaattccca tttaacaatt cggcgacgtt tatattcacg gaggctaact tcttgtttac
     9001 tagtaagctg ttgaaagaaa tggtaattta cgataatgtt ttttaccaga attcagggcg
     9061 cagattcatt cagcgcacct aaacgatagt aaagtaacaa tatattttac tagtgtaatc
     9121 acattaggta tcaacggcta tatgaattgc gttggcctat attagcatgg aatgcgaagc
     9181 ggctttatct tactgaacgc cacactggcg aaaaatgtgt tcgatagacg cagtgtcagg
     9241 aggaacgagt gaaacataaa caacgttggg cgggggcaat ctgctgtttt gtcctcttca
     9301 ttgtggtgtg cctttttctg gcgacgcaca tgaaaggcgc ttttcgggct gccgggcatc
     9361 ctgaaatcgg cttgctgttt ttcattcttc ctggagcagt tgccagtttc ttttcacagc
     9421 gtagagaagt cctgaaacct ctatttggcg caatgctggc ggcaccctgt tcaatgctca
     9481 ttatgcggct gtttttttca ccgacgcgct cattctggca agagctggca tggttactaa
     9541 gcgcggtgtt ctggtgtgcg ctgggggcac tgtgtttctt atttatcagt agtttgttta
     9601 aaccacagca cagaaaaaat cagtaaagcc ctcaacgcga gggcttgtca gacgatcagg
     9661 cgtccagatt ttctttcacc catgcagcaa aatcggtata gccgccgata tgttgctgat
     9721 cgacaaaaat ctgcggcacg gtttctacgg gtttacctgc cttttgttgt agatcttctt
     9781 tagtgatccc ttccgcacga atatctacat actgatactg aaaatcatcg cgttcattgc
     9841 tcaatttctc agccagatct tttgcacgca cacagtaagg gcaacccgaa cgaccaaaaa
     9901 taacggtttg cattatttct ctcctcatag atttatgcct gtaatgatca cgctaaaatg
     9961 tattcgctga aagtaggttt aacctgttgc attaattgct aaaagctata actgttaaac
    10021 acaatacagt gaaaagtttt agactgaagg ctcactttgc agagggaagc gtatgcgcgc
    10081 gatcggtaaa ttgcctaaag gcgtgttgat actggaattt atcggaatga tgctactggc
    10141 ggtggcgctg ctgtcggtaa gcgactccct gtcgctgcct gagccatttt ctcggccaga
    10201 agtgcagatt ctgatgattt ttctcggtgt tttgctcatg cttcccgctg cggtggtggt
    10261 tattcttcag gtggcaaaac gtcttgcccc acagctgatg aaccgtccac cgcaatattc
    10321 acgttcagaa agagaaaaag ataatgacgc caaccattga acttatttgt ggccatcgct
    10381 ccattcgcca tttcactgat gaacccattt ccgaagcgca gtgtgaggcg attattaaca
    10441 gcgcccgtgc gacgtccagt tccagttttt tgcagtgtag tagcattatt cgcattaccg
    10501 acaaagcatt acgtgaagaa ctggtgacgc tgaccggcgg gcaaaaacac gtagcgcaag
    10561 cggcggagtt ctgggtgttc tgtgccgact ttaaccgcca tttacagatc tgtccggatg
    10621 ctcagctcgg cctggcggaa caactgttgc tcggtgtcgt tgatacggca atgatggcgc
    10681 agaatgcatt aaccgcagcg gaatcgctgg gattgggcg
//
LOCUS       DAJVRF010000134        10651 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJVRF010000134 DAJVRF010000000
VERSION     DAJVRF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10651
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.134"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(95..835)
                     /locus_tag="OQN13_003222"
     CDS             complement(95..835)
                     /locus_tag="OQN13_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCS1616502.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(928..1563)
                     /gene="argO"
                     /locus_tag="OQN13_003223"
     CDS             complement(928..1563)
                     /gene="argO"
                     /locus_tag="OQN13_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCS1616503.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(1702..2562)
                     /gene="mscS"
                     /locus_tag="OQN13_003224"
     CDS             complement(1702..2562)
                     /gene="mscS"
                     /locus_tag="OQN13_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCS1616504.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(2920..3999)
                     /gene="fbaA"
                     /locus_tag="OQN13_003225"
     CDS             complement(2920..3999)
                     /gene="fbaA"
                     /locus_tag="OQN13_003225"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCS1616505.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4214..5377)
                     /gene="pgk"
                     /locus_tag="OQN13_003226"
     CDS             complement(4214..5377)
                     /gene="pgk"
                     /locus_tag="OQN13_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCS1616506.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5427..6446)
                     /gene="epd"
                     /locus_tag="OQN13_003227"
     CDS             complement(5427..6446)
                     /gene="epd"
                     /locus_tag="OQN13_003227"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCS1616507.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            6818..7249
                     /locus_tag="OQN13_003228"
     CDS             6818..7249
                     /locus_tag="OQN13_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1616508.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7272..7850
                     /locus_tag="OQN13_003229"
     CDS             7272..7850
                     /locus_tag="OQN13_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCS1616509.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            7851..8558
                     /locus_tag="OQN13_003230"
     CDS             7851..8558
                     /locus_tag="OQN13_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCS1616510.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RQAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8546..9223
                     /locus_tag="OQN13_003231"
     CDS             8546..9223
                     /locus_tag="OQN13_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCS1616511.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9217..9894
                     /locus_tag="OQN13_003232"
     CDS             9217..9894
                     /locus_tag="OQN13_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1616512.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(9866..10579)
                     /locus_tag="OQN13_003233"
     CDS             complement(9866..10579)
                     /locus_tag="OQN13_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCS1616513.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10576..>10651)
                     /locus_tag="OQN13_003234"
     CDS             complement(10576..>10651)
                     /locus_tag="OQN13_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1616514.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2538 a         2837 c         2668 g         2608 t
ORIGIN      
        1 aggtgattac gtcatggttt tacagaaaat tacagaaaaa ggaggcaata tcgggtaaag
       61 gcattagccc gacgaatacg tcgggctaca agtattattg tgctgcaggt gttttagcgg
      121 gttgttgatc cacaggttct aactggaaga ccacatcgac ctgatcatca aactgaatag
      181 cggcctgctc gtaagtttcc tgggcagaca ccggcgcggc atcggctttc atcatccgca
      241 ccattgggct gggctgatag ttggaaacat ggtagcgcac gctatatacc ggccccagtt
      301 tacgatgaaa gccgttcgcc agttcctgcg cctgatgaat cgcgttatca atcgctgcct
      361 tacgcgcttt gtctttatag gcatccggct gcgccacgcc cagcgacaca gaacgaattt
      421 cgttaagacc cgccttcagc gcgccatcca gcaaggaatt cagtttgtct aactgacgga
      481 gcgtgacttc caccgttctc acagcgcggt agcctttcag gatactttta ccatcctgat
      541 aatcataatc tggctgggtg cgtaagttcg ctgagctgat atcttttttc gcgatctgat
      601 tgagttcaag gaaggaaatg tattgtgcga cgcgctcatc tgcctgtttc ttggctgtag
      661 cggcatcctt cgcggccacg ttaacttcaa tcgcaagagt ggcaatgtct ggcaccgcat
      721 ccacacttgc cgtaccggag gtgacaatat gcggtccatc cggcaattcg ttagcctgcg
      781 ctgccatccc gctaataccc attaatgccg ccagggcgat aactttgaac ttcacgttag
      841 tctctccgtg ttaattgact tgcccaaaat tcaggacctc tggaggcaag cttagcgcct
      901 ctgttttatt tttccatcag atagtgccta actgaacaag gcttgtgcat gagcaatacc
      961 gtctctcgcc agctgcaagg ctataaacca catgacacat cccactacca gattgataat
     1021 gcgctgtgat tttgccgtgc gcaggcgcgg tgccagccag gctgcgagaa tagccagacc
     1081 aaagaaccac aggaaagagg cgctaattgt cccgagcgca aaccaacgtt ttggttcgac
     1141 gtcaagctgc ccgccaaggc tgcccagtac aacaaaagta tccaggtaaa catgcggatt
     1201 cagccaggtc actgccagca tggtggcgat aattttccat ctgccttgct tcaggacttc
     1261 ggcactagct aactcaatat tactactcat cgctgtttta aaagcgccaa aaccatacca
     1321 cagcaagaag gctacgccgc cccaggtgac cagcgccagc aaccacggcg actgcatcaa
     1381 taacgcgctg ccaccaaaaa tcccggcgca aatcaggacc aaatcgctga tagcacaaag
     1441 taaggcaatc ataatgtggt actgacgacg aatgccctga ttcatcacaa aagcattttg
     1501 tggaccgagc ggcagaatca tagctgcccc aagagcaata ccttgaaagt aataagaaaa
     1561 cacgttggat attccgaatt aagtatctga tgcagagact atatcgcggg attgttatta
     1621 gcggaaattg ataatattaa ttggcaatca gaataactaa taagagagag gccagagagt
     1681 attatctggc ctcagcgttg attacgcagc tttgtcttct ttcacccgct taaagttcac
     1741 atccatttgc gggtacggga agctgatacc ggcggcatca aattcacgtt taatacgctc
     1801 cagcacatcc cagtacacgt tttgcagatc gccgctgttg ctccagacgc ggaccacgaa
     1861 attaatcgac gatgcaccaa gttcgttcag acgcacagtc atttcgcgat ctttcaaaat
     1921 gcgatcttca gactggataa tattggtcag gatctgctta acctgatcga tatcggaatc
     1981 atacgccacg ccaataataa attcgttacg gcgaactggc tcgcgagaga agttaataat
     2041 atttccggca ataattttac cgttcggaat aacgataatt ttaccgtctg cagtacgcat
     2101 ggtggtggag aaaatctgca cactcagcac agtaccggct acgccgccga gatcaacata
     2161 ttctccggca cggaacgggc ggaacatgac aagtaacacg ccagcggcca ggttagaaag
     2221 tgacccctgc aaagccagac caacagctaa gcctgcggca ccgagtacag caatgactga
     2281 cgctgtttgt acacccacgc gtcccagggc agcgattagc gtaaaggcga taataccgta
     2341 acggactaat gcagaaagaa aatcagcaac agtggcatcg attttacggg agatcatcag
     2401 gcgattcacc gcgttggaaa tcatccgcgc gataatcaaa ccaacgatga tgatcgcgag
     2461 tgccgccacg atgtttactg cataacttag cagcagcgcc tggttagcta ccagccagct
     2521 tcccgcgccg tttatgctat cgacaacatt caaatcttcc attcaatatt ccttttcaac
     2581 tgactcaaaa tggagaaata cactccgccg ccttatgacg ggcagtctga caagggtaaa
     2641 caataaagca taattttgcc aaatagatca cagatttctc atctgaatca ccttgctttt
     2701 ggcgaaaaaa tccccgcaga gcggggctca gacagatatc aatgtgcgct ttgtcatgcc
     2761 ggatgcggcg tgaacgcctt atccggccta caaaaacctg caaattcaat atattgcagg
     2821 atacgtgtag gcctgataag cgtagcgcat caggcaattt tgcttttggc gaaaaaaaga
     2881 cccgcagagc gggccttgag ataagcagaa aggaatatct tacagaacgt cgatcgcgtt
     2941 cagttcctgg aatgcttttt ccagacgagc gatcatcgaa gtctgaccgg cacgcagcca
     3001 tacgcgcgga tcgtagtatt tcttgttcgg ctgatcttcg cctttcgggt tacccaactg
     3061 cccctgcaga taagcttcgt tcgctttgta gtagttcaga acgccttccc aggttgccca
     3121 ttgggtatcg gtatcgatgt tcatttttac tacgccgtag cttacggagt ctttgatttc
     3181 ctgagcagta gaaccggaac caccgtggaa tacgaagttc aggctgttgt gcggcaggtt
     3241 gtgtttcttg gaaacatatt cctgagaatc acgcaggatg gtcggagtca gaaccacgtt
     3301 acccggcttg taaacaccgt gtacgttacc gaaggacgca gcgatggtga aacgcgggct
     3361 gattttgctc agttcggtgt atgcgtaatc aacgtcttcc ggctgggtgt acagtgcaga
     3421 agcgtccatg tggctgttgt ccacgccgtc ttcttcacca ccggtgcaac ccagttcgat
     3481 ttccagagtc atgccgattt tggacatgcg ctccaggtat ttagagcaga tttcgatgtt
     3541 ctcttgcaga gattcttcag acaggtcgat catgtgagaa gagaacagcg gcttaccggt
     3601 agctgcgaag tgtttttcac ccgcgtccag cagaccgtcg atccacggca gcagtttctt
     3661 cgcgcagtgg tcagtgtgca ggataaccgg aacaccataa tgttcagcca tctggtgaac
     3721 gtgatgcgca ccagagatcg cgcccaggat agcagcaccc tgcggaacgt cagatttcac
     3781 gcctttacca gcgataaagg aagcaccacc gttggagaac tgaacgataa ccggcgcttt
     3841 aactttagca gcggtttcca gtacggcgtt gatggagtca gtaccgacgc agtttactgc
     3901 tggcagtgcg aagttgtttt cttttgctac ctggaaaact ttctgtacgt catcaccagt
     3961 gattacgcca ggttttacga aatcaaaaat cttagacatg tctcttgtcc tgtatcgtcg
     4021 ggccttatac tcatcatact tcaagttgca tgtgctgcgt ctgcgttcgc tcaccccagt
     4081 cacttactta tgtaagctcc tggggattca ctctcttgtc gccttcctgc aactcgaatt
     4141 atttagagta tgaaaaaatg cgtgctctaa aagcgcgctg aaacaagggc aggtttccct
     4201 gccctgtgat tttttacttc ttagcgcgct cttcgagcat cgctactgca ggcagtactt
     4261 taccttccac gaattcgagg aatgcgccgc cgccagtgga gatgtaggag attttgtcag
     4321 caatgccgaa caggtcgatt gctgccagag tgtcgccgcc gccagcgatg gagaacgctt
     4381 cgctgtctgc gatagcgtta gccacgattt cagtaccttt gcggaagttc gggaattcga
     4441 acacgccaac cggaccgttc cacagaatgg ttttcgcatt cttcaggatt tcagccagtt
     4501 cctgagcgga agcatcaccg atatccagga tctgctcgtc agctttcaca tcgttaacag
     4561 atttcagggt agccgatgca gtttcagaga actcggttgc tacgcgaaca tcagacggaa
     4621 ccgggatgtt gcaggtggtc agcagacgtt tagcttcgtc aaccaggtca gcttcgtaca
     4681 gggatttacc cacatcgtgg ccttgtgccg cgataaaggt gttagcgata ccaccaccaa
     4741 caatcagctg gtcagcgatt ttagacaggg agtccagaac ggtcagtttg gtagatactt
     4801 tagaaccacc aacgatagcc accatcgggc gagcaggttc tttcagtgct ttacccagcg
     4861 cgtccagttc agctgccaac agcgggcctg cgcacgcaac atcagcgaat ttaccgatac
     4921 cgtgagtaga agcctgcgcg cggtgagcag taccgaatgc gtccattacg aacacgtcac
     4981 acagtgcagc gtattttttg gacagggttt cgtcgtcttt cttctcgcct ttgttgaagc
     5041 gaacgttttc cagaacaacc agttcacctt cagcaacgtc aacgccgtcg aggtaatctt
     5101 taaccagacg aaccgggtta gacagtttgt ctttcaggta gttaacaacc ggcagcagag
     5161 agaattcttc gttgtactcg ccttcggtag gacgacccag gtgggaagtt accatcactt
     5221 tcgcgccttg tttcagggcc agttcgatgg tcggcagaga agcacggata cgcgcgtcgc
     5281 tggttacttt cccgtctttt actggtacgt tcagatccgc acggataaat acacgtttcc
     5341 cagcaagatc cagatcggtc atcttaatta cagacatggt gaatcctctc gttgattcta
     5401 aaagttttgc agacgctgct tgcgtcttac ctgaaagcaa cagtagccat agctaacgtc
     5461 gtgtcgagca ttcggttagc aaagccccat tcgttatcgc accagaccaa cgttttgatc
     5521 aggtgtgcgc cactgacccg ggtttgggtg ccatcgacaa tggcactgtg cggatcgtgg
     5581 ttaaaatcta cagagaccaa cggcaattcc gtatagtcaa ctataccatg aaatgcacct
     5641 tgtgctgctt tttgcagcaa caggttgact tcattggctt ttacaggttt cttcaccgtc
     5701 acgcttaaat cgattgccgt cacatttatg gttggcacac gtaccgcaat cgcttcaaag
     5761 cgatcgttaa attgcggaaa aaatcgtgtg ataccggcgg ccagtttagt atcgaccgga
     5821 atgatcgact ggctggctgc ccgggtgcga cgcaggtcag gatggtatgc atcaataacc
     5881 tgttgatcgt gcatggcgga gtgaattgtg gtcacagtgc cggactcaat accgtacgca
     5941 tcatctaaca atttgatgac gggaattatg caattcgtgg tacaggaagc gttagaaacg
     6001 atgcggtgtt ccgcacgaag ttgatcctga ttgacgccgt aaacaacggt cgcgtcgaga
     6061 tcgttactgc caggatgtga aaagagcact tttttggccc cggcggcaat atgcgcttcg
     6121 ccatgctcgc gggagccata tacgccggtg cagtcgagga ctacatcaac gccaagttca
     6181 cgccagggga gcgattgcag tgaacgttca tgcaatacgc ggatggcgtc atcaccaaca
     6241 aaaagttgat cgcgttcctg tcgtacttcc catgcaaaac ggccatggct ggtgtcatat
     6301 ttcaacaaat gcgccatgcc cgcagcatcc gccagttcgt tgattgccac cacggtaatt
     6361 tccgcccggc gtccggattc atacaaagca cgaaccacat tacgcccgat gcgaccgaag
     6421 ccatttatcg ctacgcgtac ggtcatagat ctcctgcaag gttttccctg agcaaatttg
     6481 ccagacagag taatccagca aatcgtccgg ggaaacctta cctgtcgcaa actgcgactg
     6541 attggttaat tgtcgaacat ttaatcgact gaaacgcttc agctaggata agcgaaacgt
     6601 ggaataaaag gaatgattgt ccagtcaaag aaaacaatta tctgacttac gtcacgtttt
     6661 cagccccgcc cctgtggaat ttattccact ttgcagaatc acgaataagc gtagcaaaat
     6721 cttttcgttg tgccgtcaca aaccctgact tatatcagaa tatgactgat gcagcgtcga
     6781 tattatccgc aatacgtccg agtttcggag tactccaatg atgacattgc cctacccata
     6841 ttcttccccc gcacgctttt atgcactgcg cctcctcccc gggcaggaag ttttttccca
     6901 gctacacgct tttgcacagc agcatcaact tcatgccgcc tggattgcag gctgtaccgg
     6961 tagcctgact gacgtcgcct tgcgctatgc cgggcaagaa ggcaccacgc tccttaacgg
     7021 tacattcgaa gtgatctcac taaacggcac gctggaacag agtggtgagc atcttcatct
     7081 ctgcgtttcc gatccacacg gcacgatgct tggcggtcat atgatgcccg gctgtaccgt
     7141 gcggaccacg cttgaactgg tgatcggtag ccttgaggaa ctggcgttta gccgccaacc
     7201 ttgcgcgctt tctggttacg acgagttgca tatttccccc gttaaataac tactttcaaa
     7261 gaggttttct tatggcgcgt agccactttt ccagtcaggc actggtgttg atcgttatat
     7321 ctatcgccat caatatgatc ggcgggcaac tggccagcat ggtcaaatta cctatatttc
     7381 tcgactccat cggcacactt attagcgcgg tactgcttgg tccggtgatc ggaatgctga
     7441 ccggcttact gaccaattta ctctggggat tgctgaccga cccgatcgcc gccgcatttg
     7501 cacccgtcgc gatggtcatc ggcctggtcg ccggttggct ggcgcgagcg ggttggtttc
     7561 gcaccctgcc aaaagtcgtt gtcagcggcg tgattattac gcttgcggta acggtcgtcg
     7621 ctgtaccgct gcgtactgcg ctgttcggcg gtgtcaccgg tagcggagcc gatttgttcg
     7681 ttgcctggat gcactcgatg gggcaaaacc tggtggagtc ggtggcgatt acagtgatag
     7741 gcgcgaacct ggtcgataaa attcttacag cggtgattgt ctggctttta ctgcgccagc
     7801 tgccgattcg caccacgcgc cattttccgg caatggctgc cgtgcgctaa atgcatccat
     7861 ttacctcatt aacactgtgg gcattggcgg cctgcaccac gcttatcctg cccgcgcaga
     7921 ccattttgcc gatctatagc gcggcgacct ttttttgtct tattgcgcta aaggccacgc
     7981 ggcggcaggc aaaatatgtc gcctggctga tgttctcgct gggagccggg ctgtggctgg
     8041 tacacggtgg ctggctaaca gaatggctaa gcggcacgcc gcgttcgccg gagcgttgga
     8101 cgcatgctat tacgctctgg ttgcggattc tggcgattgt ttcaacctca cagttgtgga
     8161 tgcagtacgt ccccgtgcaa tgttttatcc gcgctctgtt tgcttctcgc ctgccgccag
     8221 gcgtcgctta tctgtttgcc ggaccgttat tggtcgttga gcagttaaaa cggcaattag
     8281 cgattattca tgaagcgcag cgtgcccgcg gcgtgccgct ggatgagggc tggtatcagc
     8341 gtttacgtgc gatgcccgcg ttaattattc tgctaacaca caatgcgctt aacgatctgg
     8401 ctgtccgtgg tgcggcgctg gatatgcggg catttcggat ccacaaccga cgaacaacgt
     8461 tgtgggcacc agccgatagc accctacaac gcgtagcgcg ttacaccatg atcttgctga
     8521 tgctaacaga attcggagcg tggatatggt tacgttagaa cagttccgtt attgcccgac
     8581 acactcaacg catcctccgt tctgttatga ctttcactat gtcaaaccgg ggatggtcgc
     8641 catctttggt gacaacggca gtggcaaaag tacgctggca cagctgatgg cgggctggta
     8701 tccggacttt ctgccaggag agataacagg cacggggacg ctcctcggta cgcccattgg
     8761 gcatctcccg cttaatgaac aatccgccac catccagctc gtccagcaat cgccctattt
     8821 gcagctctcc ggctgtacct ttagcgtgga agaagaagtt gcctttgggc cagaaaatct
     8881 gtgtcttgct gaaacagaga ttatggcgcg gattgatgcc gccctgactc tgaccgagtg
     8941 ccagccgttg cgccatcgtc acccggcaac actctccggt ggagagacac aacgcgtggt
     9001 catcgcctgc gccatcgcga tgcagccaaa gctcttaatt cttgatgaag cctttagtcg
     9061 cctgacgccg caggccagcg aaatgctgtt gcagcgctta caacactggg cgcttgaacg
     9121 tggttcgctg attattcttt ttgaacgtca tcgcacacct ttcctgaact actgccagca
     9181 agcgtggcaa ttgcaaaacg gagcgctcca gccactatgt taacgttaaa tcagatatct
     9241 tatcgctggc ctagcgcagc aacggattgt ctttgcgaca tttctctgca actcaaacaa
     9301 ggtgaatggt tggcgctgac cggcgacaat ggcgcgggaa aatcgacgct gttacgagta
     9361 atggcaggtc ttcttacgcc cacagccggt acggttatgc tgcaacaaca ggcgatgaaa
     9421 aacctgaaaa accgccagcg cgcggcaaag gttggcgtac tgtttcagga agcggaaaac
     9481 caactttttc acagcaccgt cgctgacgag atcgcatttg gtttgaaact gcaaaaatgc
     9541 ccggcggatg aaatcactca acgtacccat gctgccctgc aatgttgtca gttagcagac
     9601 actgccaact cgcacccgtt ggatctgcat agtgcgcagc gacggatggt cgccgtcgcc
     9661 tgcctggaag cactctctcc gccactatta ctgctggacg agccgagtcg ggattttgac
     9721 gaaaattggt tgagcgtgtt tgaaagctgg ctggagaagt gccgccagcg aggcacgagc
     9781 gtggtagcaa tcagtcatga cgccgcattt acccggcgtc atttctctcg tgtggtgcga
     9841 ctggaagatg gtgtaatcag gaacgttaat ccaccagacg atattcaccc gtagcggtca
     9901 tcattaaggt caaatttgca ggcagactct cttccagcac ccggcgaaca ttcggcccgt
     9961 cggtacggtc ataaaaggct tctgcatctg ccagcgatac ccctcccgcc agcttgcgcc
    10021 ccaccagacg ctcccgcaac gcagtagctg gcgcgttgat aaagatcgag aaatcacaaa
    10081 attgcgcaag ctggcaccac ttctcgtcgt ccagcaacaa ccagtttcct tcgacgatga
    10141 cgagtggtgc ggtaacgtgc aacgcatctt caacaggatc atgcttttgt cgatcgtact
    10201 gcggccacgt acaatcccct tccaccacct ggcgcagatt ttccgccagt ttcgcaacgt
    10261 caaatgtctc tggtgcgcct ttgaaggggc gcaattgatg cgcgtccagc cagctattgt
    10321 aatggtgaaa accatccatc gggagcgtct gaatagcggg cagctccggg tcttgttgca
    10381 cgagatattc ccagaaggtg gttagggtag atttacccgt ccccggcgga gcgcaaagaa
    10441 aaaccaccgt acgccgctgc ggatttaccg tctgtaaggc cgccagcata cgcagcaacg
    10501 gtttatgcac gcgttctatc tcttcatcat ggtactgcgc ttgcacgttc agcccattca
    10561 ccgttaattc aattttcacg gcgttaattc ctttaaggtg gtcgttactg ccagcgtcag
    10621 cgccgttttg accattccgc taaaacgcgc c
//
LOCUS       DAJVRF010000135        10591 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJVRF010000135 DAJVRF010000000
VERSION     DAJVRF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10591
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.135"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(136..1164)
                     /gene="malI"
                     /locus_tag="OQN13_003235"
     CDS             complement(136..1164)
                     /gene="malI"
                     /locus_tag="OQN13_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCS1616515.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1339..2931
                     /gene="malX"
                     /locus_tag="OQN13_003236"
     CDS             1339..2931
                     /gene="malX"
                     /locus_tag="OQN13_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCS1616516.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDASGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2941..4113
                     /gene="malY"
                     /locus_tag="OQN13_003237"
     CDS             2941..4113
                     /gene="malY"
                     /locus_tag="OQN13_003237"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCS1616517.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4217..5218
                     /gene="add"
                     /locus_tag="OQN13_003238"
     CDS             4217..5218
                     /gene="add"
                     /locus_tag="OQN13_003238"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCS1616518.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5254..6294)
                     /locus_tag="OQN13_003239"
     CDS             complement(5254..6294)
                     /locus_tag="OQN13_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1616519.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSCVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6538..6663
                     /gene="blr"
                     /locus_tag="OQN13_003240"
     CDS             6538..6663
                     /gene="blr"
                     /locus_tag="OQN13_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCS1616520.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6928..7143
                     /gene="ydgT"
                     /locus_tag="OQN13_003241"
     CDS             6928..7143
                     /gene="ydgT"
                     /locus_tag="OQN13_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCS1616521.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7229..7669
                     /locus_tag="OQN13_003242"
     CDS             7229..7669
                     /locus_tag="OQN13_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCS1616522.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7746..8327
                     /gene="rsxA"
                     /locus_tag="OQN13_003243"
     CDS             7746..8327
                     /gene="rsxA"
                     /locus_tag="OQN13_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCS1616523.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8327..8905
                     /gene="rsxB"
                     /locus_tag="OQN13_003244"
     CDS             8327..8905
                     /gene="rsxB"
                     /locus_tag="OQN13_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCS1616524.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8898..>10591
                     /gene="rsxC"
                     /locus_tag="OQN13_003245"
     CDS             8898..>10591
                     /gene="rsxC"
                     /locus_tag="OQN13_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCS1616525.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIAR"
BASE COUNT         2430 a         2736 c         2702 g         2723 t
ORIGIN      
        1 tctcaagttg tcagaattaa acacataaac ctcctgtgaa cttcagtaaa aataaaaggc
       61 accgcctgtg tgccacattt tttatcatta accttgagga aaggacacta ccaggtatag
      121 cagataaaga gaggattatt tcgctgcaat gagccgggcg ggaataataa gattgcgtga
      181 atgcgtctct tcatgggtga ttttttgcat catgcgatcc gcaagtgtgg taccaagttc
      241 ccgcgcaggc gtgctggccc aggtaacggg tatatcatca agtgtggttg gtgtcgcatc
      301 ggtaaatgcc gccagcgaaa cttgttgctc aaagtaacga tcgactccgc tttccccgct
      361 ttgccgccct gctttcagca aaccaaacca tgcccccatc gcaatagttt cgttatagca
      421 aaccacagca ctgatggtcg ggttatgacg taaaagcgcc gtgatagctt ccgcggcttg
      481 cttctggctg gatgtgcact ccagcaccca atcgctgtga aacggcaggc caaattttag
      541 tagagttgca caatagcctc ccacccgttc tgcccgggtt aatgaggaac tttgccctcc
      601 cagccaggcg atccgctgat gcccattgcg aatgagatgc tccgtcaaca actgtgcagc
      661 ctgcatgttg tccgggcgaa ccgtatcaac atcatcaaga taactggcac gggaagcgaa
      721 aatcaccggg atagcttttt cttctgccat ccgtcgcagg tcatcgctgc ttcctgcagc
      781 cccggcaatt accacaccat cgacaccctg attcagtaac agtgaaaacc gctgtgccag
      841 ctgctcgccg tctttaccgc cgtgaagcaa aaaaaccatc cgtccctgcg cttccagagc
      901 ttccgtcaat ccggccgtca attcggcgta aaacggcgca gataaatcac ggacgatcaa
      961 accaatgacg ccgctttgcc cgccgcgcag cgccgacgcc tggcgattgc gcacaaatcc
     1021 cagctcttca atggcggcgt taacgcgttc tcctgtggcg gtagagattc gccctttgcc
     1081 actaagcacc agcgaaacgg tacttaccga cacgcccgca gccagcgcaa catcatgaat
     1141 ggttattttt ttggcggtag ccatgcagat gacctactcc ctgattatgt gacagataaa
     1201 acgttttacc ttttatttat cttatacccg ctattatcgt tgcgtaatgt gatttatgct
     1261 tcactaaaat ttgataaaac gttttatctt ctcgcgcaat ttactgaatc cagattgttc
     1321 tctacgagga gtcgttttat gacggcgaaa acagcaccga aagtcacgct gtgggagttc
     1381 ttccagcagt taggcaaaac cttcatgtta cccgtggcat tattgtcgtt ctgcggcatt
     1441 atgctcggca ttggtagttc tcttagtagc cacgatgtca tcaccctgat cccggtcctg
     1501 ggcaaccccg tgttgcaggc tatctttacc tggatgagta agattggctc gtttgctttt
     1561 agtttcctgc cagtcatgtt ctgtatcgcc atcccgctgg gtctggcacg cgaaaacaaa
     1621 ggcgtagcgg cattcgctgg cttcgtcggt tatgcggtaa tgaacctcgc ggtaaacttc
     1681 tggttgacca ataaaggcat tctgccaacc acggacgccg cggttctgaa agccaataac
     1741 atccagagca ttcttgggat ccagtcgatc gacaccggga tcctcggtgc ggtgatcgcc
     1801 ggtattatcg tctggatgct gcatgagcga ttccataata tccgcctgcc ggatgcgctt
     1861 gcattcttcg gcggtacgcg cttcgtacca atcatctcct cgctggtgat gggccttgtc
     1921 ggcctggtga ttccattagt ctggccgatt ttcgcgatgg gtattagcgg cttgggccat
     1981 atgataaaca gcgcgggtga tttcggaccg atgctgtttg gtaccggtga acgtctgctg
     2041 ttgccgtttg gtctgcatca cattctggtg gcattaattc gctttaccga cgcaagcggc
     2101 acgcaggaag tctgcggtca aaccgtcagc ggcgcattga ccatcttcca ggcgcaattg
     2161 agttgcccga ccactcacgg tttttctgaa agcgccacgc gtttcctttc gcaaggcaaa
     2221 atgcctgcgt ttctcggcgg tctgccaggt gcagcgttag cgatgtatca ctgcgcgcgc
     2281 ccggaaaatc gccataaaat taaagggctg ctgatttctg gcctgatcgc ctgtgtcgtt
     2341 ggcggcacga ccgaaccgct ggaattcctg ttcctgttcg tggcgccagt tctgtatgtc
     2401 atccacgcgc tgttaaccgg cctcggcttc actgtcatgt ctgtgctcgg cgtcaccatc
     2461 ggtaataccg acggcaatat catcgacttc gtggtgttcg gtattttgca tggtctgtca
     2521 accaagtggt acatggtgcc agtggtggcg gcaatctggt ttgtcgttta ctacgtcatc
     2581 ttccgtttcg ctatcacccg cttcaatctg aaaaccccgg ggcgcgatag cgaagtggcc
     2641 agttcaatcg aaaaagccgt tgccggtgcg ccgggtaaat caggttacaa cgttccggca
     2701 atcctcgaag ccttaggcgg tgccgacaat attgttagcc tcgataactg cattacccgt
     2761 ctgcgtttgt ctgtgaaaga tatgtcgctt gttaatgtgc aggcactgaa ggacaatcgg
     2821 gcaattggcg tagtacaact taatcaacat aacctgcagg ttgttatcgg gccacaagtt
     2881 cagtcagtaa aagatgaaat ggccggtctg atgcatactg tccaggcata aggataagat
     2941 atgttcgatt tttcaaaggt cgtggatcgt catggcacat ggtgtacaca gtgggattat
     3001 gtcgctgacc gtttcggcac tgctgacctg ttaccgttca cgatttcaga catggatttt
     3061 gccactgccc cctgcattat cgaggcgctg aatcagcgcc tgatgcacgg cgtatttggc
     3121 tacagccgct ggaaaaacga tgagtttctc gcggctattg cccactggtt ttccacccag
     3181 cattacaccg caattgatac gcagacggtg gtgtatggcc cttctgtcat ctatatggtt
     3241 tcagaactga ttcgtcagtg gtctgaaaca ggtgaaggcg tggtgatcca cacgcccgcc
     3301 tatgacgcat tttacaaggc cattgaaggt aaccagcgca cagtaatgcc cgttgcttta
     3361 gagaagcagg ctgacggctg gttttgcgat atgggcaagc tggaagccgt tttggcgaaa
     3421 ccagaatgta aaattatgct cctgtgtagc ccacagaatc ctaccgggaa agtgtggacg
     3481 tgcgatgagc tggagatcat ggctgacctg tgcgagcgtc atgatgtgcg ggttatttcc
     3541 gatgaaatcc atatggatat ggtctggggc gagcagccgc atattccctg gagtaatgtg
     3601 gcgcggggag actgggcgtt gctgacgtcg ggatcgaaaa gtttcaatat tcccgcgctg
     3661 accggtgctt acgggattat agaaaatagc agcagccgtg atgcctattt atcggcactg
     3721 aaaggacgtg atgggctttc ttccccttcg gtactggcgt taactgccca tatcgccgcc
     3781 tatcagcaag gtgcaccgtg gctggatgcc ttacgcgtct atctgaaaga taacctgacg
     3841 tatatctcag ataaaatgaa cgccgcgttt cctgaactca actggcagat cccgcaatcc
     3901 acttatctgg cctggcttga tctacgtccg ttgaatattg acgacaacgc gttgcaaaaa
     3961 gcgcttatcg agcaagaaaa agtcgcgatc atgccggggt atacctatgg tgaagaaggt
     4021 cgtggttttg tccgtctcaa tgccggctgc ccacgttcga aactggaaaa aggtgtggct
     4081 ggattaataa acgccatccg cgctgttcgc taaccccaat tgcgcaacgt aaaaaatcgt
     4141 tgcgcaatcg tggattttta ccctgctttg tttttataat ggtgcgcact tttatatcca
     4201 gaaaaagagt gcgaccatga ttgataccac cctgccatta actgatatcc atcgccacct
     4261 tgatggcaac attcgtcccc agaccattct tgaacttggc cgccagtata atatctcgct
     4321 tcctgcacaa tccctggaaa cactgattcc ccacgttcag gtcattgcca acgaacccga
     4381 tctggtgagc tttctgacca aacttgactg gggcgttaaa gttctcgcct ctcttgatgc
     4441 ctgccgccgc gtggcatttg aaaacattga agatgcagcc cgtaacggcc tgcactatgt
     4501 cgagctgcgt ttttcaccag gctacatggc aatggcacat cagctgcctg tagcgggtgt
     4561 tgtcgaagcg gtgatcgatg gcgtacgtga aggttgccgc acctttggtg tgcaggcgaa
     4621 gcttatcggc attatgagcc gaaccttcgg cgaagccgcc tgtcagcaag agctggaggc
     4681 ctttttagcc caccgtgacc agattaccgc acttgattta gctggtgatg aacttggatt
     4741 cccgggaagt ctgttccttt ctcacttcaa ccgcgcgcgt gatgcgggct ggcatattac
     4801 cgtccatgca ggcgaagctg ccgggccgga aagcatctgg caggcgattc gtgaactggg
     4861 tgcggagcgt attggacatg gcgtaaaagc cattgaagat cgggcgctga tggattttct
     4921 cgccgagcaa caaattggta ttgaatcctg tctgacctcc aatattcaga ccagcaccgt
     4981 agcagagctg gctgcacatc cgctgaaaac gttccttgag catggcattc gtgccagcat
     5041 taacactgac gatcccggcg tacagggagt ggatatcatt cacgaatata ccgttgccgc
     5101 gccagctgct gggttatccc gtgagcaaat ccgccaggca cagattaatg gtctggaaat
     5161 ggctttcctc agcgcagagg aaaaacgcgc actgcgagaa aaagtcgccg cgaagtaaca
     5221 aaaatggatg gtgcaatttg caccatccat ttttcacgca aggcacaacg tcgcgcgatg
     5281 tttggcggat tcgatgccca gctcaatcaa ctccattacc tggattgcct ggcttgccgg
     5341 aaccggattt tcaccatcgc catttaacgc atcacgaatc gccgcatagt aagccggata
     5401 attcccaggc accgtcaaca gcgtttcttc gacacgttcc tcaccttcca cgcgggtaag
     5461 tacgccatca cgcatatcgt agccccagtc ttcctgcggc agacgctcgc catttttcag
     5521 acgttcttcc tgtggatcga ggccatattt cacacaactg cctcgggatc catgcacgat
     5581 ataccgtgct gactcagcgg ctgccagcat ggtaccgtgt aaaatgactc gccgctgtgg
     5641 ataggacaag atggcgtgga aataatcggt cgactgcgct ccgggccgta actgtgccaa
     5701 atctaccgtc atgctgaccg gtaaaccaaa tagcgtaatg gcctgatcaa gaagatgcgg
     5761 tgctaaatcg taccagatac cgctgcctgg gccgccctgt tcacgccaac gatcgcgcac
     5821 ctgcggacgg aagcggtcaa aatgagactc aaagtaagca acttcaccca gcacgccttc
     5881 cgcgagcaaa ccttttagcg tcaagaaatc gctatcccaa cgacggttat ggaatacaga
     5941 cagcacacgc cccaggcttt ttgccagcgc atccagctct cgcgcttgtg acagtgtcac
     6001 ggtaaagggt ttatcaacga ccacatgttt acccgcctca agcgccgctt tggctaacgg
     6061 gaaatgggta tcgttgggtg taggaatgac aatcaggtct atgttgggat cgttaaacag
     6121 atgcttcggc tcagagacaa ccgtaaccgt tggccagtcg gcttttactt ttgtttcatc
     6181 actgctggag attaccgcca gttccagccc gggcgtgccc gcaatcaggg gcgcatggaa
     6241 ggttttgctc gcataaccat acccaatcaa cccaacacgg atgttgtcgc tcatgtcatt
     6301 gcctctcatt gaagtatgat ggctatttga cactatcctt tacccacgct caacagttta
     6361 ataacctgcc agcaataagg gatgttgttt aacttaagtc aaaaaaaata gcgaattttc
     6421 caacgacaaa agctaaatat cgcaaaaacc tcagtaaaaa tcttgctgga gctattattg
     6481 ctaagtaaca tttaccccct gaagttaatg gatcaatcaa gagagatgtg ggctgtaatg
     6541 aatcgtctta ttgaattaac aggttggatc gttcttgtcg tttcagtcat tcttcttggc
     6601 gtggcgagtc acattgacaa ctatcagcca cctgaacaga gtgcttcggt acaacacaag
     6661 taagctctgc acttgtggag cgacatgctg cccgtccggg tgcatgtttt cacttgtcgg
     6721 atattaaacc aggaatttat tatcttgttc gatgttgttg gtgattgtca gggatagtaa
     6781 agcgcgttac tcttttcaga gcagtttttc atccctgaat atttgccatc agcgcattct
     6841 tattattgta ataacacaag cgttcacttt tggtgaaatt ttatcatcac tctacccctt
     6901 tggtattaat tattttatgg accttttatg actgttcagg actacttatt aaaatttcgc
     6961 aaaatcagtt cactcgaaag tctggaaaaa ctctacgacc atcttaatta caccctgacg
     7021 gacgatcagg aactgatcaa tatgtatcgt gctgccgatc accgtcgcgc agagctggtt
     7081 tctggcgggc gtttgtttga cctcggccag gtaccgaagt ccgtctggca ctatgtccaa
     7141 taaagaaagt agcgatcggt tataaaagcc ttataataac gcctctaaaa ttagatttgc
     7201 ctgtttgcgt cgctattctg gagaacctat gaccaccaca acgccacaac gtattggagg
     7261 ctggttgctt ggcccgttgg cctggctttt agtcgctctg ttgagtacga cgctggcgtt
     7321 gttgctgtat accgctgcgt tatcttctcc gcaaacattt caaacgctcg gcggacaagc
     7381 actgacgaca caaatcttat ggggcgtttc ttttattacc gctatcgcca tgtggtatta
     7441 cacactgtgg ttgaccatcg ctttctttaa acgtcggcgt tgtgttccta aacactatat
     7501 tatctggctg cttatttccg tattgctggc agtaaaagca ttcgcttttt cacccgtgga
     7561 agatggcata gccgttcgcc agttgctgtt tactttgttg gcaaccgcat tgatcgtccc
     7621 ctatttcaaa cgctcgtcgc gggtaaaagc gacgtttgtg aatccgtaat aaccttacag
     7681 ttaacctgtt gtcgcctgct ctggattaac ggataatagg cggctttttt atttcaggcc
     7741 gaaaaatgac tgactacctg ttactgtttg tcggaactgt actggtcaat aactttgtac
     7801 tggtcaagtt tctcggtctc tgtccgttta tgggggtttc caaaaaactg gaaaccgcga
     7861 tgggcatggg gctggcaaca acgtttgtga tgacgctggc gtctatttgc gcctggctta
     7921 tcgatacgtg gattttgatc ccacttaatc tgatttacct gcgcaccctg gcatttattc
     7981 tggtgattgc tgtggtcgtg cagttcaccg agatggtggt gcgcaaaacc agcccggtgc
     8041 tttaccgtct gctggggatt tttttgccgc ttatcaccac caactgtgca gtgctcggcg
     8101 tggcgttgct gaatatcaat ctcgggcaca atttcttgca gtcggcgctg tacggttttt
     8161 ccgccgctgt cggtttctcg ctggtgatgg tgctcttcgc cgccatccgc gaacgccttg
     8221 ctgtggctga tgtcccggca ccttttcgcg gtaatgccat tgcgttaatt accgcaggtc
     8281 ttatgtctct ggcctttatg ggctttaatg gtttggtgaa gttgtaatga atgctatctg
     8341 gattgccgtt gccgccgtga gcctgctggg cctggcgttt ggcgccattc tgggttatgc
     8401 ctcccgccgt tttgcggtgg aagacgatcc ggtcgttgag aaaattgacg aaatcttacc
     8461 gcagagccag tgtggtcagt gcggttatcc cggctgtcgc ccctacgcgg aagccatcag
     8521 ctgtaacggt gaaaaaatca accgttgcgc cccaggtggc gaagctgtga tgctaaaaat
     8581 tgccgagttg cttaatgtcg agccgcagcc gctggatggc gaagcgcaag agctaacgcc
     8641 tgcgcggatg gtggcggtta ttgatgaaaa taactgtatt ggctgcacta aatgtattca
     8701 ggcttgtccg gtagacgcca tcgttggcgc tacccgtgcc atgcatacgg taatgagtga
     8761 tctctgtacg ggctgcaatt tatgtgttga tccgtgcccg acgcactgca tctcgttgca
     8821 accggtcgca gaaacacctg actcctggaa atgggatctg aacaccattc ccgtgcgtat
     8881 cattcccgtg gaacaccatg cttaagttat tctctgcatt cagaaaaaat aaaatctggg
     8941 atttcaacgg cggcatccat ccaccggaga tgaaaaccca gtccaacggt acacccctgc
     9001 gccaggtacc actggcgcag cgttttgtta ttccactgaa acagcatatt ggcgctgaag
     9061 gtgagttgtg cgttagcgtc ggcgataaag tattgcgcgg ccagccgctt acccgtggtc
     9121 gcggcaaaat gctgcctgtt cacgcgccca cctcgggtac cgttacggct attgcgcccc
     9181 actctacggc tcatccttca gctttagctg aattaagcgt gattattgat gccgatggtg
     9241 aagactgctg gatcccgcgc gacggctggg ccgattatcg cactcgcagt cgcgaagagt
     9301 taatcgagcg catacatcag tttggtgttg ccgggctggg cggtgcagga ttcccgacag
     9361 gcgttaaatt gcagggtggc ggagataaga ttgaaacgtt gattatcaac gcggctgagt
     9421 gcgagccgta cattaccgcc gatgaccgtt tgatgcagga ttgcgcggct caggtcgtag
     9481 agggtattcg cattcttgcg catattctgc agccacgcga aattcttatc ggcattgaag
     9541 ataacaaacc gcaggcgatt tccatgctgc gcgcggtgct ggcggactct aacgatattt
     9601 ctctgcgggt gattccaacc aaatatcctt ctggcggtgc taaacaatta acctacattc
     9661 tgaccgggaa gcaggttcca catggcgggc gttcatccga tatcggcgta ttaatgcaaa
     9721 acgtcggcac tgcttatgca gtgaaacgtg ccgttattga tggcgagccg attaccgagc
     9781 gtgttgtaac cctgactggc gaagcaatcg ctcgcccggg caacgtctgg gcacggctgg
     9841 ggacgccagt gcgtcattta ttgaatgatg ccggattctg cccctctgcc gatcaaatgg
     9901 tgattatggg tggcccgcta atgggcttta ccttgccatg gctggatgtc ccggtcgtaa
     9961 agattaccaa ctgtctgttg gctccctctg ccaatgaact tggcgaacca caggaagaac
    10021 aaagctgcat ccggtgtagc gcctgtgctg acgcctgccc tgcggatctt ttgccgcaac
    10081 agttgtactg gttcagcaaa ggtcagcaac acgataaagc taccacgcat aacattgctg
    10141 attgcattga atgtggggct tgcgcgtggg tttgcccgag caatattccc ctggtgcaat
    10201 atttccgtca ggaaaaagct gaaattgcgg ctattcgtca ggaagaaaag cgcgccgcag
    10261 aagccaaagc gcgtttcgaa gcgcgccagg ctcgtctgga gcgcgaaaaa gcggctcgcc
    10321 ttgaacgaca taagagcgca gccgttcaac ctgcagccaa agataaagat gcgattgctg
    10381 ccgctctggc gcgggtgaaa gagaaacagg cccaggctac acagcctatt gtgattaaag
    10441 cgggcgaacg cccggataac agtgcaatta ttgcagcacg ggaagcccgt aaagcgcaag
    10501 ccagagcgaa acaggcagaa ctgcagcaaa ctaacgacgc agcaaccgtt gctgatccac
    10561 gtaaaactgc cgttgaagca gctatcgccc g
//
LOCUS       DAJVRF010000136        10421 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJVRF010000136 DAJVRF010000000
VERSION     DAJVRF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.136"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            143..2011
                     /gene="kup"
                     /locus_tag="OQN13_003246"
     CDS             143..2011
                     /gene="kup"
                     /locus_tag="OQN13_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCS1616526.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2178..2597
                     /gene="rbsD"
                     /locus_tag="OQN13_003247"
     CDS             2178..2597
                     /gene="rbsD"
                     /locus_tag="OQN13_003247"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCS1616527.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2605..4110
                     /gene="rbsA"
                     /locus_tag="OQN13_003248"
     CDS             2605..4110
                     /gene="rbsA"
                     /locus_tag="OQN13_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HCS1616528.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4115..5080
                     /gene="rbsC"
                     /locus_tag="OQN13_003249"
     CDS             4115..5080
                     /gene="rbsC"
                     /locus_tag="OQN13_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCS1616529.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5105..5991
                     /gene="rbsB"
                     /locus_tag="OQN13_003250"
                     /pseudo
     CDS             5105..5991
                     /gene="rbsB"
                     /locus_tag="OQN13_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6117..7046
                     /gene="rbsK"
                     /locus_tag="OQN13_003251"
     CDS             6117..7046
                     /gene="rbsK"
                     /locus_tag="OQN13_003251"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCS1616530.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVTAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7050..8042
                     /gene="rbsR"
                     /locus_tag="OQN13_003252"
     CDS             7050..8042
                     /gene="rbsR"
                     /locus_tag="OQN13_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCS1616531.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8008..9435)
                     /gene="mdtD"
                     /locus_tag="OQN13_003253"
     CDS             complement(8008..9435)
                     /gene="mdtD"
                     /locus_tag="OQN13_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCS1616532.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9458..10150)
                     /locus_tag="OQN13_003254"
     CDS             complement(9458..10150)
                     /locus_tag="OQN13_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCS1616533.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2550 a         2514 c         2752 g         2605 t
ORIGIN      
        1 aaaggggggg tatttttctt tgagccaggt taatgtggcc gcatttagga gtacgatttt
       61 gccgttaatc gtgcatactg tgcgcttttt tgtgggccaa gggactaagc acacatttca
      121 tatttcaacg aaagactagt ctatgagcac tgataataag caatcattgc ccgcgattac
      181 cctcgcggcg attggagttg tctacggcga tattggtacc agcccgttat atacacttcg
      241 tgaatgtttg tccggccagt ttggttttgg cgttgaacgc gatgccgtgt ttggcttttt
      301 atcgctgatc ttctggctgc taatctttgt ggtttccatt aaatatctca ccttcgtgat
      361 gcgggcagat aacgccggtg agggggggat cctgacgttg atgtcgcttg ccgggcgtaa
      421 tacgtcggcg cgaaccacat caatgctggt gattatgggg ctaatcggcg gcagcttttt
      481 ctatggtgaa gtcgtcataa cacccgctat ttcggtgatg tcagccattg aaggtctgga
      541 aatcgtcgcc ccgcagctgg atacctggat agttcccctc tcaattatcg ttctcacttt
      601 attatttatg attcaaaagc atggcacggc tatggtcggt aagctgtttg cgccgatcat
      661 gctgacctgg tttttgattc tggcaggact ggggttacgt agcattattg ctaacccgga
      721 agtgctgcat gcgctgaatc cgatgtgggc gatgcatttc ttccttgaat acaaaacggt
      781 ttcttttatt gcattagggg cagtggtgtt gtcgattacg ggggtcgagg cgctgtatgc
      841 tgatatgggg cactttggta agttccctat tcgcctggcg tggttcaccg tcgtattacc
      901 ttccttgacc cttaattact tcggtcaggg agcgctgttg ttaaagaacc cggaagcgat
      961 taagaacccg ttcttcctgt tggcaccgga ctgggcgctg atcccgctgc tgatcatcgc
     1021 cgcactggcg acggtaattg cctcgcaggc ggttatctct ggcgtcttct cattgacgcg
     1081 tcaggcggta cgtctgggat atttgtcgcc gatgcgcatt attcacacct ccgaaatgga
     1141 gtcagggcaa atctatattc cgtttgtgaa ctggatgctc tatgtcgcgg tcgtgattgt
     1201 gattgtcagc tttgagcact ccagcaacct ggcggcggcg tacgggattg cggtgaccgg
     1261 aaccatggtg ctgacgtcta ttctctcgac taccgtggca cgtcagaact ggcactggaa
     1321 taagtatttt gttgcgctga tcctgattgc tttcctttgt gtcgatattc cattgttcac
     1381 cgctaacctc gataaactgc tctccggcgg ctggttgcca ttgagcctcg gtactgtgat
     1441 gtttatcgtg atgaccacct ggaagagcga gcgtttccgc ttgctgcggc ggatgcatga
     1501 acatggtaac tctctggaag cgatgattgc ttcgctggag aaatcaccgc ctgttcgcgt
     1561 gcccgggacc gcggtgtata tgtcgcgtgc aatcaacgtc attccctttg cgctgatgca
     1621 taaccttaaa cataacaagg tattgcatga gagggtgatt ctgttaactc tgcgcaccga
     1681 agacgcgcca tatgtccata acgtccgtcg ggtacagatt gaacaactgt cgcccacttt
     1741 ctggcgcgtg gtggcaagtt atggttggcg agaaacgcca aacgtggaag aagttttcca
     1801 ccgctgcggt ctggaaggat taagttgccg gatgatggaa acctccttct ttatgtcgca
     1861 tgagtcgttg atcctcggca aacgcccgtg gtatttgcgt ctgcgcggca agctgtactt
     1921 gctgctgcaa cgtaatgcgc tgcgtgcacc agatcaattt gaaatcccgc caaacagggt
     1981 tatcgaactg ggtactcagg tcgaaatcta acgctagacg cctcctttct ccataagggg
     2041 gcgtttttgt tttcatggtt aatcaccatg taaaacgttt cgaggttgat cacatttccg
     2101 taacgtcacg atggttttcc caactcagtc aggattaaac tgtgggtcag cgaaacgttt
     2161 cgctgatgga gaaaaaaatg aaaaaaggca ccgttcttaa ttctgatatt tcatcggtga
     2221 tctcccgtct gggacatacc gatacgctgg tggtgtgtga tgctggttta cccatcccca
     2281 aaagtacaac gcgtatcgat atggcattaa cccagggtgt accttctttt atgcaggtgc
     2341 tgggcgtcgt cacaaatgaa atgcaggtcg aggctgccat tatcgcggaa gagatcaaac
     2401 aacataatcc gcaactccac gaaacgttgc tcactcacct tgagcagctg caaaaacacc
     2461 agggaaatac cattgaaatt cgttacacca cgcatgaaca attcaaacaa caaaccgcag
     2521 aaagtcaggc ggtaattcgc agcggagaat gttctccgta tgcgaatatc attctctgtg
     2581 ctggcgtgac gttctgaggc cgtcatggaa gcattacttc agcttaaagg catcgataaa
     2641 gccttcccgg gcgtaaaagc cctctcgggc gctgcgttaa atgtctatcc gggccgcgtg
     2701 atagcgctgg tgggcgaaaa cggcgcgggt aaatccacca tgatgaaagt gcttactggt
     2761 atctatactc gcgatgccgg cacgctttta tggctgggga aagaaacgac atttaccggg
     2821 ccgaaatctt cccaggaagc cgggattggg attatccatc aggaactgaa cctgatcccg
     2881 cagttgacca ttgccgaaaa cattttcctc ggtcgtgagt ttgttaatcg ctttggcaaa
     2941 attgactgga aaaccatgta tgccgaagcg gataaattgc tggctaaact taacctgtgc
     3001 tttaaaagcg acaagctggt gggcgatctt tccatcggtg accagcaaat ggttgaaatc
     3061 gccaaagtgc tgagctttga gtcgaaagtc atcattatgg atgaaccgac cgatgcgctg
     3121 accgataccg aaaccgaatc cctgttccgc gtcatccgcg agctgaaatc gcaaggccgc
     3181 ggtattgtct atatctccca ccgcatgaaa gaaatcttcg agatttgcga tgacgttacc
     3241 gtttttcgtg acgggcaatt tattgctgag cgcgaagtgg catcactgac cgaagattcg
     3301 ctgattgaga tgatggtggg ccgcaagctg gaagatcaat atccacacct ggacaaagcg
     3361 ccgggagata tccgcctgaa agtcgataat ctctgcggac ctggcgttaa cgatgtctct
     3421 tttactttac gcaaaggcga aattcttggc gtctctggtt tgatgggcgc aggtcgtacc
     3481 gaactgatga aagtgctcta cggcgcacag ccgcgcacca gcggttacgt caccctggat
     3541 gggcatgaag tcgttacccg ttcaccgcag gatggcctgg caaacggcat tgtgtatatc
     3601 tccgaagacc gtaaacgtga cggtttagtg ttgggcatgt cagtaaaaga gaacatgtcg
     3661 ctgacagcgc tgcgctactt cagccgcgct ggcggcagtt tgaagcatgc cgatgaacag
     3721 caggcagtga gtgatttcat tcgtctgttt aatgtgaaaa caccgtcgat ggaacaggca
     3781 attggtctgc tttccggtgg caatcagcaa aaagtggcga ttgcccgcgg tctgatgaca
     3841 cgccccaaag tgttgatcct tgatgaacct acccgtggcg tagatgtcgg cgcgaaaaaa
     3901 gagatctatc aactgattaa ccagttcaaa gccgatggct tgagcatcat tctggtgtca
     3961 tcggagatgc cagaagtatt aggcatgagc gatcgcatca tcgtcatgca tgaagggcat
     4021 ctcagcgggg aatttactcg tgagcaggcc acccaggaag tgttaatggc tgccgctgtg
     4081 ggcaagctta atcgcgtgaa tcaggagtaa aaaaatgaca acccagactg tctctggtcg
     4141 ccgttatttc acgaaagcgt ggctgatgga gcagaaatcg cttatcgctc tgctggtgct
     4201 gatcgcgatt gtctcgacgt taagcccgaa ctttttcacc atcaataact tattcaatat
     4261 tctccagcaa acctcggtga acgccattat ggcggtcggg atgacgctgg tgatcctgac
     4321 gtcgggcatc gacttgtctg taggttctct gttggcgcta accggcgcag ttgctgcatc
     4381 tatcgtcggc attgaagtca atgcgctggt ggctgtcgct gctgctctcg cgttaggtgc
     4441 cgcaattggt gcggtaaccg gggtgattgt agcgaaaggt cgcgttcagg cgtttatcgc
     4501 tacgctggtt atgatgcttt tactgcgcgg cgtgaccatg gtttatacca acggtagccc
     4561 agtgaatacc ggctttactg agaacgccga tctgtttggc tggtttggta ttggtcgtcc
     4621 gctgggcgta ccgacgccag tctggatcat ggggattgtc ttcctcgcgg cctggtacat
     4681 gctgcatcac acgcgtctgg ggcgttacat ctacgcgctg ggcggcaacg aagcggcaac
     4741 gcgtctttct ggtatcaacg tcaataaaat caaaatcatc gtctattctc tttgtggtct
     4801 gctggcatcg ctggccggga tcattgaagt ggcgcgtctc tcctccgcac aacccacggc
     4861 ggggactggc tatgagctgg atgctattgc tgcggtggtt ctgggcggta cgagtctggc
     4921 gggcggaaaa ggtcgcattg ttgggacgtt gatcggcgca ttaattcttg gcttccttaa
     4981 taatggattg aatttgttag gtgtttcctc ctattaccag atgatcgtca aagcggtggt
     5041 gattttgctg gcggtgctgg tagacaacaa aaagcagtaa taacgactac aggacatctt
     5101 gaatatgaac atgaaaaaac tggctaccct ggtttccgct gttgcgctaa gcgccaccgt
     5161 cagtgcgaat gcgatggcaa aagacaccat cgcgctggtg gtctccacgc ttaacaaccc
     5221 gttctttgta tcgctgaaag atggcgcgca gaaagaggcg gataagcttg gctataacct
     5281 ggtggtgctg gactcccaga acaacccggc gaaagagctg gcgaacgtgc aggacttaac
     5341 cgttcgcggc accaaaattc tgctgattaa cccgaccgac tccgacgcag taggtaatgc
     5401 tgtgaagatg gctaaccagg cgaacatccc ggttatcact cttgaccgcc aggcaacgaa
     5461 aagtgaagtg gtgagccaca ttgcttctga taacgtactg ggtggcaaaa tcgccggtga
     5521 ttacatcgcg aagaaagcgg gtgaaggtgc caaagttatc gagctgcaag gcattgctgg
     5581 tacatccgca gcccgtgaac gtggcgaagg cttccagcag gccgttgctg ctcacaagtt
     5641 taatgttctt gccagccagc agattttgat cgcactaaag gtttgaacgt aatgcagaac
     5701 ctgttgaccg ctcatccgga tgttcaggct gtattcgcgc agaatgatga aatggcgctg
     5761 ggcgcgctgc gcgcactgca aactgccggt aaatcggatg tgatggtcgt cggatttgac
     5821 ggtacaccgg atggcgaaaa agcggtgaat gatggcaaac tagcagcgac tatcgctcag
     5881 ctacccgatc agattggcgc gaaaggcgtc gaaaccgcag ataaagtgct gaaaggcgag
     5941 aaagttcagg ctaagtatcc ggttgatctg aaactggttg ttaagcagta gttttaatca
     6001 ggttgtatga cctgatggtg acataaatac gtcatcgaca gatgaacgtg taatataaag
     6061 aaaagcaggg cacgcgccac cctaacacgg tggcgcattt tatggacatc ccgaatatgc
     6121 aaaacgcagg cagcctcgtt gttcttggca gcattaatgc tgaccacatt cttaatcttc
     6181 aatcttttcc tactccaggc gaaaccgtaa ccggtaacca ctatcaggtt gcatttggcg
     6241 gcaaaggcgc gaatcaggct gtgactgctg ggcgtagcgg tgcgaatatc gcgtttattg
     6301 cctgtacggg tgatgacagc attggtgaga gcgttcgcca gcagctcgcc actgataaca
     6361 ttgatattac tccggtcagc gtgatcaaag gcgaatcaac aggtgtggcg ctgatttttg
     6421 ttaatggtga aggtgagaat gtcatcggta ttcatgccgg cgctaatgct gccctttccc
     6481 cggcgctggt ggaagcgcaa cgtgagcgta ttgccaacgc gtcagcatta ttaatgcagc
     6541 tggaatcacc actcgaaagt gtgatggcag cggcgaaaat cgcccatcaa aataagacta
     6601 tcgttgcgct taacccggct ccggctcgcg aacttcctga cgaactgctg gcgctggtgg
     6661 acattattac gccaaacgaa acggaagcag aaaagctcac cggtattcgt gttgaaaatg
     6721 atgaagatgc agcgaaggcg gcgcaggtac tgcatgaaaa aggtatccgt actgtactga
     6781 ttactttagg aagtcgtggt gtatgggcta gcgtgaatgg tgaaggtcag cgcgttcctg
     6841 gattccgggt gcaggctgtc gataccattg ctgccggaga tacctttaac ggcgcgttaa
     6901 tcacggcgtt gctggaagaa aaaccattgc cagaggcgat tcggtttgcc catgctgccg
     6961 ctgcgattgc cgtaacacgt aaaggcgcac aaccttccgt accgtggcgt gaagagatcg
     7021 acgcattttt agacaggcag aggtgacgct tggctacaat gaaagatgtt gcccgcctgg
     7081 cgggcgtttc tacctcaaca gtttctcacg ttatcaataa agatcgcttc gtcagtgaag
     7141 cgattaccgc caaagttgaa gcggcgatta aagaactcaa ttacgcgcca tcagccctgg
     7201 cgcgtagcct caaactcaat caaacacata ccattggcat gttgatcact gccagtacca
     7261 atcctttcta ttcagaactg gtgcgtggcg ttgaacgcag ctgcttcgaa cgcggttata
     7321 gtctcgtcct ttgcaatacc gaaggcgatg aacagcggat gaatcgcaat ctggaaacgc
     7381 tgatgcaaaa acgcgttgat ggcttgctgt tactgtgcac cgaaacgcat caaccttcgc
     7441 gtgaaatcat gcaacgttat ccgacagtgc ctactgtgat gatggactgg gctccgttcg
     7501 atggcgacag cgatcttatt caggataact cgttgttggg cggagactta gcaacgcaat
     7561 atctgatcga taaaggtcat acccgtatcg cctgtattac cggtccgctg gataaaactc
     7621 cggcgcgcct gcggttggaa ggttatcggg cggcgatgaa acgtgcgggt ctcaacattc
     7681 ctgatggcta tgaagtcact ggtgattttg aatttaacgg cgggtttgac gccatgcgcc
     7741 aactgttatc acatccgctg cgtcctcagg ccgtctttac cggaaatgac gctatggctg
     7801 ttggcgttta ccaggcgtta tatcaggcag agttacaggt tccgcaggat atcgcggtga
     7861 ttggctatga cgatatcgaa ctggcaagct ttatgacgcc accattaacc actatccacc
     7921 aaccgaaaga tgaactgggg gagctggcga ttgatgtact catccatcgg ataacccagc
     7981 cgacccttca gcaacaacga ttacaactta ctccgattct gatggaacgc ggttcggctt
     8041 agatttacgc tgtcttttga tcaaattatt accatcggtt gttttcagaa gcatgaacat
     8101 tgctgctgaa gcaacagtaa taatgcccat cgtgataaac gtatagtgga attgttcgac
     8161 agtcgttgtg ccttccatcc cttcataaac gcgaaggacg gccgcactta cagcaacgcc
     8221 taaactaatc gacagttgct gcgtgaccgc cagaacactg ttaccgctgc tggcgttgtc
     8281 atcggtcaga tcggcaagtg tgatggtatt catcgcggta aattgcgtcg acatagccat
     8341 ccctaatata aacaacggca agatcagcat ccatatcgcc attgccggtg attgcaaaga
     8401 gaactgagcg atcattagcc caataatcac cgtgatcccc actaacgtat ggcgatagcc
     8461 cagacgacgt aagacttggg taaccatcga ttttgcaata atggaaccta acgctgtcgg
     8521 tgccatcata cagccggcaa taaacgcctg ataaccaaat cctacctgta acatcaatgg
     8581 cataaggaac gatacacaac cggtccccag acgggttgca atattgccta cgataccgat
     8641 tgagaaagtg cgggttttaa ataaatctaa tgaaattaat gggtttggcg tgtgtcgcgc
     8701 atggagaata tagagaagca gtaacccgat gctggtgaca attaccgtca aggcaatcca
     8761 gctggcgaca atcttttccc cgaatagctc tattccgctt gagaagagaa caaggctgag
     8821 gccaaacagc aaaaagccag tgatatcgaa tctgcgtcgt gcggtggtga aattgggcat
     8881 atgtttgcgc gcgtaaagaa ggcccgcaat acctatgggg atattgatta aaaatatcca
     8941 gtgccaggtt gcccaggtga ccagcacgcc gccaagaacg gggcctaaaa ttggccccac
     9001 cagacccggc atggcgacaa aattcaatac tggaagaagt tcattacgag gataagcgcg
     9061 cagtaaggcc agccgagcaa caggcatcat cattgcgccg cctatcccct gaataacccg
     9121 gaagacaacc agctgtggta gcgaattaga aagtgcgcag gccagagaac ccaatgtgaa
     9181 cagactcacg gcaagggtaa aaatgcgacg cgtaccgaag cgatcggcta gccatccgct
     9241 taccggaata agcatcgcca ccgtcagcgt ataactgatg atggctgatt gcatcgcgag
     9301 aggagaacga ttaaggctat gagcgattgc gggtaaggcg gtattcagaa tagtggcatc
     9361 aagtgcctgc atgaagaagg ccatcgccgc gatccacggc aaacccgcca tactgcgctt
     9421 ctttttatcg ctcattcaat gtcttgttat cgggttgtca cttatcaggt gagcgtagca
     9481 gcgcctgaca agctttaaat gccgcgtcgc catcgctttg gacaatcgca tcgacaatcg
     9541 cctggtgcag atttagcttt atcactgtgt cgctggtaat tgacgtgaag taagtgtgat
     9601 aaaccgaatg gaatagcgag gcgaatgagg tcaaaaacgg attggcgctc atttcataga
     9661 tatgctcatg ccaggccata tcgacttcga tccagcgttc acggcgaaag ttctctttta
     9721 atgccgccat ttcggccatt aacgtattga gatgcgcctt ctgttccgcg gtgccaaccg
     9781 ttgctgccag taggcaggct tgcggctcca gacagatacg cataaccaga aagtgatcga
     9841 tgacctgatg aaagttctct tctgtcatcc accaggtaag caattcctga tcaagaaaat
     9901 tccagttcga ttgtggcatg acccgagtac caattcgcgg tcgcggtaaa accatccctt
     9961 ttgccgttaa cgttttgacc gcttcgcgta ccgctgtacg actcactcca aattgctcgc
    10021 ccagctcaat ctcaccaggc aaaatggtgc cgggttcata ttcacctttt aagatccgtt
    10081 gcgccagctt ctcagccaga acatacgaaa ggtttttctg tgcagctaac tgttgtgcgc
    10141 ttaaaggcat tacttatctt cctttttctt tttattcctc cttagtatgc caccaggaag
    10201 tgtgattacg gttgcaaaaa cggcaaattg cttgttttat ggtacattaa cggggctttt
    10261 gctgaaaaaa tgcgcggtca gaaaattatt ttaaatttcc tcttgtcagg ccggaataac
    10321 tccctataat gcgccaccac tgacacggaa caacggcaaa cacgccgccg ggtcagcggg
    10381 gttctcctga gaatctcaac agagaaagca aaaataaatg c
//
LOCUS       DAJVRF010000137        10345 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJVRF010000137 DAJVRF010000000
VERSION     DAJVRF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10345
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.137"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(43..1047)
                     /gene="cra"
                     /locus_tag="OQN13_003255"
     CDS             complement(43..1047)
                     /gene="cra"
                     /locus_tag="OQN13_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCS1616534.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1129..1215)
                     /locus_tag="OQN13_003256"
     CDS             complement(1129..1215)
                     /locus_tag="OQN13_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCS1616535.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1227..1718)
                     /gene="ilvN"
                     /locus_tag="OQN13_003257"
     CDS             complement(1227..1718)
                     /gene="ilvN"
                     /locus_tag="OQN13_003257"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS1616536.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1721..3445)
                     /gene="ilvI"
                     /locus_tag="OQN13_003258"
     CDS             complement(1721..3445)
                     /gene="ilvI"
                     /locus_tag="OQN13_003258"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCS1616537.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3564..3608
                     /locus_tag="OQN13_003259"
     CDS             3564..3608
                     /locus_tag="OQN13_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616538.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3763..4707)
                     /gene="leuO"
                     /locus_tag="OQN13_003260"
     CDS             complement(3763..4707)
                     /gene="leuO"
                     /locus_tag="OQN13_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCS1616539.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5367..5453
                     /gene="leuL"
                     /locus_tag="OQN13_003261"
     CDS             5367..5453
                     /gene="leuL"
                     /locus_tag="OQN13_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCS1616540.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5546..7117
                     /gene="leuA"
                     /locus_tag="OQN13_003262"
     CDS             5546..7117
                     /gene="leuA"
                     /locus_tag="OQN13_003262"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCS1616541.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7117..8208
                     /gene="leuB"
                     /locus_tag="OQN13_003263"
     CDS             7117..8208
                     /gene="leuB"
                     /locus_tag="OQN13_003263"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCS1616542.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8211..9611
                     /gene="leuC"
                     /locus_tag="OQN13_003264"
     CDS             8211..9611
                     /gene="leuC"
                     /locus_tag="OQN13_003264"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCS1616543.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFNGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKTGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9622..10227
                     /gene="leuD"
                     /locus_tag="OQN13_003265"
     CDS             9622..10227
                     /gene="leuD"
                     /locus_tag="OQN13_003265"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCS1616544.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
BASE COUNT         2713 a         2719 c         2524 g         2389 t
ORIGIN      
        1 cgccagaggg aaattcacct ggcgcgtatt tttgttcgcg gcttagctac ggctgagcac
       61 accgcggcga tagagattac gtttaatgcg cgttaaacca ggttttggct tacgcggttc
      121 gtccaggctt gccaggacaa tctccagcac acgctctgcg acatcgcggt gacgttgagc
      181 cactgccagc accgggcact gtaagaagtc gagcagttcg ttatcgccaa aggtggcaat
      241 tgccaggtca gaaggcagtt taccatcgcg acgtagcgtg acatccatca ccccttgcaa
      301 caacgcaaac gacgttgtga acagcgcctg cggcatcgga tgcgtttcca gccatttttc
      361 gaataactgg gcagccacct cccgctcata gctgttggca tacaggaaat gcacttcgcg
      421 cggatcatct ttccaggcag tacggaaacc ttgttcacgc aggaagctga cagaaagctc
      481 cggcagcgcg ccaagataaa gcaccgtctc ggcgggaaac ttacgtaact cttccgccag
      541 catttcggca tcatcctgat cggcaccaac cacgctggtg aagtgttcac gatcgagggc
      601 gcggtccagc gcgacaatcg ggaacgggtc gttagcccag cgttgataaa aaggatgctc
      661 aggaggcaac gacgtcgaaa caataatggc atcgacctga cgctgtaaaa ggtgctcaat
      721 acagcgcatt tcgttgtctg gctgatcttc tgagcaggca attagcagtt gataaccccg
      781 ttgccgcgcc tggcgttcaa gatagttagc gatgcgggta tagctggtgt tctccagatc
      841 ggggatcaca agaccaatag aacgtgtgcg tccagcacga agcccagctg ccacggcgtt
      901 cgggtggtaa ttgtgctcac gcaccacagc catgactttt tcaacggttt tgtcgctcac
      961 acggtattgc ttcgctttgc cgttaataac atagcttgca gtggtccgcg acactcccgc
     1021 cagccgagcg atttcatcca gtttcacaat tgccccttgc gtaaaatgta aaaaccatag
     1081 ccattgtact ggtatgggtt aaattcatta acatctaagc gcataaaatt aactgcggca
     1141 actgctttta ttccaggttc ccactgattt cgcaaaaaaa gcccaacgtg acatgttggg
     1201 ctgcaaattg cgcattcaga tcattctcaa cgcattattt tatcgccgcg cgaaagtccg
     1261 accacaccag aacgagcaac ttccacaatt ttcgccacct cgcgaatcga tgctaaaaat
     1321 gcatcaagct tatcgctggt gcctgctaat tgaacggtat aaagcgaggg tgtgacatcg
     1381 ataatttgcc cacggaatat ttccgtatta cgtttcactt cgtcacgccc gtaaccgctg
     1441 gcctgaattt tcaccagcat gatttcccgc tcaacatgcg cgccctgccc caactcactc
     1501 acgcgcaaga catcgaccag cttgtgtaat tgcttttcga tctgctcaag tactttttca
     1561 tcgcccacgg tctggatggt catacgcgat aatgtcggat catcggttga cgcaacggtc
     1621 aggctttcaa tgttgtagcc acgctgggaa aaaaggccaa tcacgcggga taacgcgcct
     1681 gattcatttt cgagtaagac tgataatatc cggcgcataa tcaggttctc tccgttttgc
     1741 ttaaccacat ttcatccatt ccgcccccgc gaatctgcat cgggtagacg tgctcgctgc
     1801 catcgacggt aacatcaaca aacaccaggc gattattgtg cacctgttcc agcgcctcgc
     1861 taagtttgct ttccagctca tgcggatgag aaatctggat cccgacgtga ccataggctt
     1921 ccgccagacg gacgaaatcg ggtagcgatt gcatataaga ttgtgaatga cggccggaat
     1981 agatcatgtc ctgccactgc ttcaccatcc ccagatagcg gttattgaga ttcaccacca
     2041 gtacgggcaa ctcgtattgc aacgcggtag acagttcctg gatgttcatc tgaatactgc
     2101 cgtcgccagt gacgcaaacc acggtttctt ctggcaacgc cattttgacg cccagtgccg
     2161 caggtaaacc aaaacccatc gtgccgaggc caccggaatt gatccagcga cgcggtttgt
     2221 cgaatggata ataaagtgca gcaaacatct ggtgctgccc gacatcggac gtcacgtaag
     2281 cgtctccctt cgtcaaccgc caaagagtct cgatcaccgc ctgcggttta atcttttcac
     2341 tgtgagtgtc atatttcagg cactgacgag cgcgccactg ttcaatttgc tgccaccagt
     2401 cgcggatctc atccagtggt tgatgggcgg attcttgcga caagagttca agcatttgtt
     2461 cgaggacctg gcgagcatcc cccacaatcg ggatatccgc agtcacggtt ttagaaatgg
     2521 aagtaggatc aatatcgata tgcagaacag tggcatttgg gcagtacttt gccagattgt
     2581 tcgtcgttcg gtcatcaaat cgtaccccga cggcgaaaat cacatccgcg ttatgcatcg
     2641 tcatattggc ttcgtaggta ccgtgcattc ccagcatgcc cagtgcctga cgatgcgttg
     2701 ccggaaacgc tcccagcccc atcaatgagg aaacaacggg cagattcaac gcctccaccg
     2761 tttctttcaa ctgctgatgg cagcccgccg tgattgcccc accgcctacg tagacaaccg
     2821 gtttttttgc cgctaccagc gtttgcagag cgcgcttaat ttgcccttta tgtccggtag
     2881 tagtgggatt gtaagaacgc atactgaccg actccggcca gacatagggt aatttgttcg
     2941 ccggattaag aatatctttc ggtaaatcaa cgactactgg tccaggacga ccacttgccg
     3001 ccagccagaa agcctttttc agcacctgcg gaatgtcttc cgtttgctta accagaaaac
     3061 tgtgtttaac cactggtcgc gaaatcccca ccatgtcgca ctcctgaaag gcatcgtaac
     3121 ctatcaacga ggtcgctacc tgcccggaaa ggacaactaa tggaatggaa tccatataag
     3181 cggtggcgat gccagtaatc gcattggtcg cccctggacc cgacgttacc agcacgacgc
     3241 cgacttcccc ggtcgcgcgc gccagaccat cggccatatg cactgccgcc tgctcatgac
     3301 gaactaaaac atgatcgata ccacccacgg tatgcaatgc atcgtaaata tcaaggactg
     3361 cgcctccggg ataaccgaat acttgtttaa cgccctgatc gataagcgat cggacgacca
     3421 tctcggctcc agacaacatc tccatggcct gcctcactgt ttgacggaaa aaatgtgtaa
     3481 aacacattaa ccgtccgatc ttgcttaagc aattggcagc cagagacata aacgcagggt
     3541 aataagaata aacaggagga aaggtgaaaa gaaacgataa aatcctccat tacgaaggat
     3601 taaattgata ataaatcggc tgaatcccac aacttacagc aatttctcta tttttttaat
     3661 taatcctaaa atagagagcc cacacattca gaataaaatt ctacattcaa taaaaaatac
     3721 agaataaacc agacattcat gtctgaccta ttctgcattc agttagcgtt tgcaaattga
     3781 gactaattgc tcttccatcc actgatggcc tttatcgcgc ccggcagctt catgccagga
     3841 gagataacag gttctgctat tttgttttaa cggcagcggt aatacctgta attctaagga
     3901 ttcagcgaac tcttcagcca gccaacgcgg cgcaatagcg accaaatgcg tttgcgacac
     3961 cacgctaagt acgctcatca ttgccatgcc ctgatacgcg atactggctt gcttatctac
     4021 cgtgtcatac caaggttgac taaatgacgc gaaacgatcg agcgaaaccg ccgcatgttg
     4081 ttcgttataa acatcatgtt tcagtaacgg gcccttaatt gttggatgat ttttgctggc
     4141 taccagcacc atttcatctt taaataatgg cacgctggta aattcaggac gatggaagtc
     4201 ttcataacta atcacaaact ccgtttcctg ataacgcagc tgatgttcag tgttctgatt
     4261 taatgaagac ttgaacataa catgtatatt tggcgcaatc tgctcaatgt gattataaat
     4321 ctgcgaggtc agaatgctgt ctaacgggct gcaaacacaa agatgaaata cacgttcact
     4381 gctcgcgggt tcaaaacctg aaccaggcaa ttcattttgt actagttgca atgcctgacg
     4441 aactgagcca aaaagttgaa atgcgcgagc agtcggttga ataccacggc cataacgaac
     4501 aaaaagctcg tcattaaaca tcaccttcag gcgtgcaaca gcgttactga ccgcaggttg
     4561 cgacataccc agaacatgag cggcacgggt aatgttttgc tcctgcatca cggcatcgaa
     4621 aacggttaat aagttgagat cgaccatgcg tagctgtggc ttgcttaact ccgccgtctc
     4681 tgaatgatct gtttgtacct ctggcatact taactccact gtcacactta actccctttc
     4741 ccttattgga atgcagagaa ataatcctga agaatatgat ttatcatgca tataaaataa
     4801 gaaaaagcaa aatgagtaaa attcggaaaa cataaagata cgggttctga taaaaacaga
     4861 atccattgcc gtggaatgag tcatttacca cagcataaga atccataatg tttttgttgc
     4921 gaaaacaatc taatcataac taccgcgaat actcaatcat ctacaaaatg gattaaatgt
     4981 gcttatttaa taattaattt atatatttaa tgcattaatt cttaacatta attgatcaat
     5041 aatattcacc aaatcaatat caaaaaaaat cgcaaaacat ataattcaat acaaatcatc
     5101 aggataggtt ttgcaacgcg tgcattttgt cccctttttc ctcgttgatt agatgcaaaa
     5161 atttatgctg aaatatgtca accgatgaaa agcgtcggta gttaagcaga aattaatatc
     5221 gcttacttta accaccgcag cacaattagc taattttacg gatgcagaac tcacgctggc
     5281 gggacgtttt tattgcgtca gggttgacat ccgtttttgt atccagtaac tctaaaagca
     5341 tatcgcattc atctggagct gatttaatga ctcacatcgt tcgctttatc ggtctactac
     5401 tactaaacgc atcttctttg cgcggtagac gagtgagcgg catccagcat taagccagca
     5461 cgcagtcaaa caaaaaaccc gcgccattgc gcgggttttt ttatgcccga agcgaggcgc
     5521 tctaaaagag acaaggacct aaaccatgag ccagcaagtc attattttcg ataccacatt
     5581 gcgcgacggt gaacaggcgt tacaggcaag cttgagtgtg aaagaaaaac tgcaaattgc
     5641 gctggccctt gagcgtatgg gtgttgacgt gatggaagtc ggtttccccg tctcttcgcc
     5701 gggcgatttt gaatcggtgc aaaccatcgc ccgccaggtt aaaaacagcc gcgtatgtgc
     5761 gttagctcgc tgcgtggaaa aagatatcga cgtggcggcc gaatccctga aagtcgccga
     5821 agccttccgt attcatacct ttattgctac ctccccaatg cacatcgcca ccaagctgcg
     5881 cagcaccctg gacgaggtga tcgaacgcgc tatctatatg gtgaaacgcg cccgtaatta
     5941 caccgatgat gttgaatttt cttgcgaaga tgccgggcgt acacccattg ccgatctggc
     6001 gcgagtggtc gaagcggcga ttaatgccgg tgccaccacc atcaacattc cggacaccgt
     6061 gggctacacc atgccgtttg agttcgccgg aatcatcagc ggcctgtatg aacgcgtacc
     6121 taacatcgac aaagccatta tttccgtaca tacccacgac gatttaggcc tggcggtcgg
     6181 caactcactg gcggcggtac atgccggtgc acgccaggtg gaaggtgcaa tgaacgggat
     6241 cggcgagcgt gccggtaact gttcgctgga agaagtcatc atggcgatta aagttcgtaa
     6301 ggatattctc aacgtccaca ccgccattaa tcaccaggag atatggcgca ccagccagtt
     6361 agttagccag atttgtaata tgccgatccc ggcaaacaaa gccattgttg gcagcagcgc
     6421 attcgctcac tcctccggta tccaccagga tggcgtgctg aaaaaccgcg aaaactacga
     6481 aatcatgaca ccagaatcta ttggtctgaa ccaaatccag ctgaatctga cctctcgttc
     6541 ggggcgtgcg gcggtgaaac atcgcatgga tgagatgggg tataaagaaa gtgaatataa
     6601 tttagacaat ttgtacgatg ctttcctgaa gctggcggac aaaaaaggtc aggtgtttga
     6661 ttacgatctg gaggcgctgg ccttcatcgg taagcagcaa gaagagccgg agcatttccg
     6721 tctggattac ttcagcgtgc agtctggctc taacgatatc gccaccgccg ccgtcaaact
     6781 ggcctgtggc gaagaagtca aagcagaagc cgccaacggt aacggtccgg tcgatgccgt
     6841 ctatcaggca attaaccgca tcactgacta taacgtcgaa ctggtgaaat acagcctgac
     6901 cgccaaaggt cacggtaaag atgcgctggg tcaggtggat attgtcgcca actacaacgg
     6961 tcgccgcttc cacggcgtcg gcctggccac cgatattgtc gagtcctccg ccaaagccat
     7021 ggtgcacgta ctgaacaata tctggcgtgc cgcagaagtc gaaaaagagt tgcaacgcaa
     7081 agctcaacac aacgaaaaca acaaggaaac cgtgtgatgt cgaagaatta ccatattgcc
     7141 gtattgccgg gggacggtat tggtccggaa gtgatgaccc aggcgctgaa agtgctgaat
     7201 gccgtgcgca accgctttgc gatgcgcatc accaccagcc attacgatgt aggcggcgca
     7261 gccattgata accacgggca accactgccg cctgcgacgg ttgaaggttg tgagcaagcc
     7321 gatgccgtgc tgtttggctc ggtaggcggc ccgaagtggg aacatttacc accagaccag
     7381 caaccagaac gcggcgcgct gctgcctctg cgtaagcgct tcaaattatt cagcaacctg
     7441 cgcccggcaa aactgtatca ggggctggaa gcattctgtc cgctgcgtgc agacattgcc
     7501 gctaacggct tcgacatcct gtgcgtgcgc gaactgaccg gcggcatcta tttcggtcag
     7561 ccaaaaggcc gcgaaggtag cggacaatat gaaaaagcgt ttgataccga ggtgtatcac
     7621 cgttttgaga tcgagcgtat tgcccgcatc gcgtttgaat ctgctcgtaa acgtcgccac
     7681 aaagtcacct caatcgacaa agctaacgtg ctgcaatcct ctattttatg gcgtgaaatc
     7741 gtcaacgaga tcgccacgga atacccggat gtcgaactgg cgcatatgta catcgacaac
     7801 gccaccatgc agctgattaa agatccatca cagtttgacg ttctgctgtg ctccaacctg
     7861 tttggcgaca ttctgtctga cgagtgcgca atgatcactg gctcgatggg gatgttgcct
     7921 tccgccagcc tgaacgagca aggttttggt ctgtatgaac cggcaggcgg ctcagcacca
     7981 gatatcgcag gcaaaaacat cgccaacccg attgcgcaaa ttctgtcgct ggcactgctg
     8041 ctgcgctaca gcctggatgc cgatgatgcg gcttgcgcca ttgaacgcgc cattaaccgc
     8101 gcattagaag aaggcattcg caccggggat ttagcccgtg gcgctgccgc cgttagtacc
     8161 gatgaaatgg gcgatatcat tgcccgctat gtggcagaag gggtgtaatc atggctaaaa
     8221 cgttatacga aaaattattc gacgctcacg tagtgtacga agccgaaaac gaaaccccgc
     8281 tgttatatat cgaccgccac ctggtgcatg aagtgacctc accgcaggcg ttcaatggtc
     8341 tgcgcgccca cggtcgcccg gtacgtcagc cgggcaaaac cttcgccact atggatcaca
     8401 acgtctctac ccagacgaaa gacattaatg cctgtggtga aatggcgcgc atccagatgc
     8461 aggagctgat taaaaactgc aaagaatttg gcgtcgaact gtatgacctg aatcacccgt
     8521 atcaggggat cgtccacgta atggggccgg aacagggcgt caccttgccg gggatgacca
     8581 ttgtctgcgg cgactcgcat accgccaccc acggcgcgtt tggcgcactg gcctttggta
     8641 tcggcacttc cgaagttgaa cacgtactgg caacgcaaac cctgaaacag ggccgcgcaa
     8701 aaaccatgaa aattgaagtc cagggcaaag ccgcgccggg cattaccgca aaagatatcg
     8761 tgctggcaat tatcggtaaa accggtagcg caggcggcac cgggcatgtg gtggagtttt
     8821 gcggcgaagc aatccgtgat ttaagcatgg aaggtcgtat gaccctgtgc aatatggcaa
     8881 tcgaaatggg cgcaaaaacc ggtctggttg caccggacga aaccaccttt aactatgtca
     8941 aaggccgttt gcatgcgccg aaaggcaaag atttcgacga cgccgttgcc tactggaaaa
     9001 ccctgcaaac cgacgaaggc gcaactttcg ataccgttgt cactctgcaa gcagaagaaa
     9061 tttcaccgca ggtcacctgg ggcaccaatc caggccaggt gatttccgtg aacgacaata
     9121 ttcccgatcc ggcttcgttt gccgatccgg ttaaacgcgc gtcggcagaa aaagcgctgg
     9181 cctatatggg gctgaaaccg ggcattccgc tgaccgaagt ggctatcgac aaagtgttta
     9241 tcggttcctg taccaactcg cgcattgaag atttacgcgc ggcagcggag atcgccaaag
     9301 ggcgaaaagt cgcgccaggc gtgcaggcac tggtggttcc cggctctggc ccggtaaaag
     9361 cccaggcgga agcggaaggt ctggataaaa tctttattga agccggtttt gaatggcgct
     9421 tgcctggctg ctcaatgtgt ctggcgatga acaacgaccg tctgaatccg ggcgaacgtt
     9481 gtgcatccac cagcaaccgt aactttgaag gccgccaggg gcgcggcggg cgcacgcatc
     9541 tggtcagccc ggcaatggct gccgctgctg ctgtgaccgg acatttcgcc gacattcgca
     9601 acattaaata aggagcacac catggcagag aaatttatca aacacacagg cctggtggtt
     9661 ccgctggatg ccgccaatgt cgataccgat gcaatcatcc cgaaacagtt tttgcagaaa
     9721 gtgacccgta cgggttttgg cgcgcatctg tttaacgact ggcgttttct cgatgaaaaa
     9781 ggccaacagc caaacccgga cttcgtgctg aacttcccgc agtatcaggg cgcttccatt
     9841 ttgctggcac gagaaaactt cggctgtggc tcctcgcgtg agcacgcgcc ctgggcattg
     9901 accgactacg gctttaaagt ggtgattgcg ccgagttttg ctgacatctt ctacggcaat
     9961 agctttaaca accagctgct gccggtgaaa ttaagcgatg cagaagtgga cgaactgttt
    10021 gcgctggtga aagccaatcc ggggatccat ttcgacgtgg atctggaagc gcaagaggtg
    10081 aaagcgggag agaaaaccta tcgctttacc atcgatgcct tccgccgcca ctgcatgatg
    10141 aacggtctgg acagtattgg gcttaccttg cagcacgacg acgccattgc cgcttatgaa
    10201 gcaaaacaac ctgcgtttat gaattaatcc ccttgcccgg tcaaatgacc gggctttccg
    10261 ctatcgtcca cgtcatcaaa cgtctttaac ctttgcggtt aaaaataatg cgacaacaga
    10321 aataaccgca attacccagt agaca
//
LOCUS       DAJVRF010000138        10318 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJVRF010000138 DAJVRF010000000
VERSION     DAJVRF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10318
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.138"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..416)
                     /locus_tag="OQN13_003266"
     CDS             complement(<1..416)
                     /locus_tag="OQN13_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616545.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVR"
     gene            589..1467
                     /gene="yihU"
                     /locus_tag="OQN13_003267"
     CDS             589..1467
                     /gene="yihU"
                     /locus_tag="OQN13_003267"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCS1616546.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1492..2379
                     /locus_tag="OQN13_003268"
     CDS             1492..2379
                     /locus_tag="OQN13_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCS1616547.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2412..3422
                     /locus_tag="OQN13_003269"
     CDS             2412..3422
                     /locus_tag="OQN13_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCS1616548.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3492..4925
                     /locus_tag="OQN13_003270"
     CDS             3492..4925
                     /locus_tag="OQN13_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1616549.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5024..6373
                     /locus_tag="OQN13_003271"
     CDS             5024..6373
                     /locus_tag="OQN13_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCS1616550.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6363..7250
                     /locus_tag="OQN13_003272"
     CDS             6363..7250
                     /locus_tag="OQN13_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCS1616551.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRIFPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7391..7990
                     /gene="yihX"
                     /locus_tag="OQN13_003273"
     CDS             7391..7990
                     /gene="yihX"
                     /locus_tag="OQN13_003273"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCS1616552.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            7984..8856
                     /locus_tag="OQN13_003274"
     CDS             7984..8856
                     /locus_tag="OQN13_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCS1616553.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            8853..9290
                     /gene="dtd"
                     /locus_tag="OQN13_003275"
     CDS             8853..9290
                     /gene="dtd"
                     /locus_tag="OQN13_003275"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCS1616554.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9335..10276
                     /gene="fabY"
                     /locus_tag="OQN13_003276"
                     /gene_synonym="yiiD"
     CDS             9335..10276
                     /gene="fabY"
                     /locus_tag="OQN13_003276"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCS1616555.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2372 a         2430 c         2770 g         2746 t
ORIGIN      
        1 cagcgaacat cggccagaat cagatcataa cgagagaaat cgatcgcttc taaccattcg
       61 gcatcagtac cgagatcagg acttggataa ttaacgataa tccgttcgcc atgctgatca
      121 acgaggatcg ctgattgcga cgaacgggcg ttaggatatc ggcggcaaaa tgcggtattg
      181 acgccccaac cctcaagttc tgcaagtaag gtatttccac aactgtcatc gccgacgcga
      241 ccgatgaaat caacctccac gccaagcttt gcgattgcaa cagcggctgt tgctgccggg
      301 ccgccaccaa tctcaagata atgatttgcc tgatatttcc cgccgccctc cggcagagtg
      361 ggcaaagaat aaattctgtc ctgaactgtg atacccacgc atgcaattct ggtcatattt
      421 gtcttgtcct gtttaatttt gtcgtctttg gtcaatttaa atgacgcaaa tgatcaaatt
      481 agtgatgcgt gtcgtgtttt tgaacataaa ttacaaatat gctcaaaaac agacgaaaac
      541 gaattttcaa ttgtcattat tttgtcaatg tcagacagga gagtattcat ggcaaccatt
      601 gcatttctcg ggctggggca gatgggtagc ccaatggcta acaatctatt gcaaaaaggg
      661 cattcattgc aggtatttga tgtcaatgca caggccgtcg atgcgctagt tacgcagggg
      721 gccactgcgg cacaaacccc agcggaagca gcagcaggcg ctgaatttat catcactatg
      781 cttcccaatg gtgacattgt tcgacaggtt ctgttggggg aaaaaggcgt ctgtgaggct
      841 gtttcatctg acgcgttatt gattgatatg tcgactattc atcccttaca gaccgatgca
      901 ttaatacggg agttacaaga aaaaggcatc agtatgatgg acgctcccgt gggcagaacg
      961 tccgtgaatg cgattgatgg cacattgctg attttagcgg gtggcactga tgagcagatt
     1021 gcacgtgcac gcccgattct catgtgcatg ggtaatgagc tggtcgaggc tggcggccca
     1081 ggaatgggga tccgcgtgaa gctaattaac aactatatga gtattgcgct taatgcgctg
     1141 tcctccgagg ccgctgtgtt atgtgagtca ctgggattaa atcttgatgt cgcaattaag
     1201 gtgatgagtg gtacggcagc agggaaagga cattttacga ccacctggcc tggcaaagtg
     1261 ctgaaaggcg atctttcacc cgccttcatg gtggatctgg cgttgaaaga tctgcgtatt
     1321 gcagttgacg tcgctcggga gacgggggca ccgctcaata tggggattgc agcagaaagt
     1381 tattacgctg cagcaagtca agacggcaaa gggcggcagg actggagtgc cctgctaaat
     1441 caggttcgcc agcaggctgg tctttaattt tatctattaa caggagtttc tatgtcatcc
     1501 cacttcaccc catttacgct gaaagatatc tcccgcccag gtggtggatt tgccatgtta
     1561 gctgtcgatc agcgtgaggc catgcgcttg atgtttgcgg cagcagggca gccaaaaccc
     1621 atcgctgaca gtgtactcac cgattttaaa gttgccgcga cccgaatcct ttctccttat
     1681 gcttctgccg ttctggcgga taaacaattt tgtcttgagc aaatagtcga gcagggcgct
     1741 gttgctaatt cttgcggcct gattgtcgct gccgatcttt ttatcccagg taatggcatc
     1801 ccggttgata gcgttgaaat agatatgtcc gtcgacccgc ataaagcccg tgaaatgggg
     1861 gctaaggcaa tgaaattact tgtactctgg cgtgaagatg aacccgcaga ggaacgtctg
     1921 gcaatggttg ataaatttgt tcgtcgctgc cgtagtgccg ggctggtgag cattattgaa
     1981 cctgtggtgc gtccacctcg tcgcggttgg gactttgatc gtgaaagcac cattgttgcc
     2041 gccgccgcag agcttggtgg aacagaggct gatctttaca aagccgagat gccattaggg
     2101 ggcaaaggcg atgaaaaaac cttacttgcc gcctgtcagc aacttaacga tcagatgaag
     2161 atgccgtggg taattctttc ttctggcgtt gatgccgata tttttggccg tgcagtaagt
     2221 attgccatga aaggtggtgc gtcgggcttc ctggctggac gagcggtttg ggcgtcggtt
     2281 gttggtgcac aagatccaca aactatgctg cgcgatgtct ctgttcctcg tttacaacgg
     2341 ttggcagaaa ttgtcgatga aggtatcgcg caacgctaat cccctatgga tatcgattct
     2401 ggagaataac aatggataaa attacccagg ttttattttc agatattggg aaagtcacca
     2461 ctcaatatgt tgaagtacca caccaggaac ttaaaccgca cgaagtgcgg attgcgcctg
     2521 tgttctatgg gatatgcggt tcggatctgc atgttctgaa aggcggtcat ccgtttgcca
     2581 aaccacctgt cgtccccggt catgaaattg cagcgcgcgt tacggaagtt ggcagcgacg
     2641 ttaaaaatgt acagccgggc gatcatgttg tggtcgatcc catcatggct tgcatggaat
     2701 gccgagcctg caaagcagga cgttttaatc tttgtgaacc accgcaggtt gctggttttc
     2761 gcgcaccggg ctttgctcgc tcacaacaca ttgttcctgc gcgtaattgc catgtcgcac
     2821 cagcctcttt accgctaaaa atgttggcct ttgccgaacc ggcggcttgt gcccgtcact
     2881 gcgttaaccg aatgccgaaa gcttctctgg aaagcgtact ggtaattggt gccggaacga
     2941 taggcttatc catcgtgcag gcactgcgca ttatgggggc aggtaagatt accgtgattg
     3001 aacctgacgc tgccaaacgc gcgctggcgt taaagctggg cgcagcagaa gtttgggcac
     3061 caggtgagct ggccgcagat gtgcgattta cgggggcgat tgatgtcgtt gcagcgcagg
     3121 ccacgcttaa cgatgcatgt acccgtgtat atgccggagg caccgtcgtg tgcatgggcg
     3181 taccaagtgg gccgcgtgaa ataccattac cgatgatgca acgtttcgag cgtgacttgc
     3241 tcaactctgg catgtacatc cctgaagatt tcgatgctgt tatcgaatgg ctggcggatg
     3301 ggcggtttga taccagtgaa ctggttaccg atttatttgc cattgaggat gcagcggcgg
     3361 catttgaacg cgcgcagcaa aatgactcca taaaggtcat gctgcaattt gcgccggaat
     3421 gattttctaa atctctgtct ggcgctgcgt cacgtcagcg ccaccctaca acaattaaaa
     3481 aggacactgc gatgagtcaa atcaccagtc ctgcaactta ttcaattagt cggccacagg
     3541 acgtaattga tattgttaat aagaactctg caatcaacac cagtatcggc gttattttta
     3601 ttgctttggg cggcatattg attgatgcct accaggcggc gatggtgggg tttggtaata
     3661 aatacattgc cgctcaattc ggcatttcgc cgggtcttgc tgcaaccgtc aatgcatcag
     3721 tattaatcgc cgcgttaatt ggcggtttat tagcgaaccg ggtaataaac cgctttgggc
     3781 aaaagcgggc atttattatt ggcatggggc tgtgcaccat cggtgctgca gcggtagcta
     3841 ttgctcccag tatctggtgg gtgctggtgt gccgcgtcat catgggcttt ggtttaggca
     3901 tcgacttccc tttggcaacc aatgccgtgg cagagcttcg tggttcaacg tcgaagaaaa
     3961 ccggaacgtc ggtcaacctc tggcaaatgg cctggtatgt ttcgacaacc gttgtttatt
     4021 tggtactttt gccgctgctt ctgtcgggta tcgctgaaga acaattgtgg cgttacggaa
     4081 tattcatcgg agctattttt gcagtcatct tcatgatttt gcgttacttc tttattggtg
     4141 aatccgcaat gtgggccgca cgcgtcgggc gttaccagga agcgtgcgac attctgggaa
     4201 aacgttatgg tgttcaggct cgcgttgcgg catcgggtac aacagaagcg aaattctcgg
     4261 aaaaagcaga gaataaatac agtggtggat atggcatctt atttaatgat cgttaccgca
     4321 aacgcaccat tcttggctgt gtcgtggcaa ccatgcaggc gtggcaatat aacgccgtag
     4381 gtgtttatct tcctcttacg ttggcgggaa taataagtgg cgggcttact ggcgcgttga
     4441 ccggttctgc cgtcgtgaat gccctttgtg gggtgacagg cgggatgatc ggctcgttta
     4501 ttctccaacg actgggtact cgccgacagt cgatgtatgg atttgctgtt gtgaccttag
     4561 cattgctgtc gttaggcgca ctggcaacga ctaatccctg gctgtcttta gggttattgg
     4621 gatcaattat tttcttccat tcagcgggtc ctggtgggct gggcatgacc attgccacac
     4681 tctcttatcc tcccgctatt cgccctactg gggtcggatt tgcccgcgct attatgcgca
     4741 caggggcaat tgcaggactc attttctggc cgatgctgtg gggtgcgttg aaaactgaag
     4801 cgttttactg gttggcaatc gtgccattcc tggggttcct gacctgcgta ttgattaatt
     4861 gggaaccact gggtgcaaat gttgatgctg aggatgcaga ggttctggct gaattgaaga
     4921 aataaattaa tgcgcatcgg cttaaaccga tgcgcattaa ttcaaactgc ccacgtttag
     4981 tggttcgtat cccagatttc tcgcctacca ggagaggaaa attatgtctg aaaagttacc
     5041 cgcaccgcgt gaagggcttt ccggtaaagc catgagacgt gtcgttatgg gcagtttcgc
     5101 aggcgcgtta atggaatggt atgacttctt tatttttggc acggcagcag gactggtatt
     5161 tgcaccgctg ttttatccag acagcgatcc ttttataggt cttatagccg cctttgcgac
     5221 ctttggtgtc ggttttctga ctcgtccttt aggtggcatc gtctttggtc attttggcga
     5281 taaaatcggg cgaaaaataa cacttatctg gacgctggct attgtcggtt gctccacatt
     5341 tttgattggt tttattccga cgtatcagga aattggcatt tgggcaccgc ttgtattaat
     5401 ggtgctgcgt ttgattcagg gctttggtct tggtggcgag tacggcggag cggcattgat
     5461 gaccatagaa tcagcgcccg aatcccgacg cggattttta ggttcactcc cgcaaacggc
     5521 tgcgtctgtg ggtattatgc tggcaaccgg tatttttgct ctctgtaacc attttcttac
     5581 ttcggagcag tttctctcct ggggatggcg cattccgttt tggctttctg cggtgatgtt
     5641 gatcgttggg ctgtttatcc gcctgcatac cgaagaaaca ctggattttc aaaagcaaaa
     5701 aacgacgaat aataaagaaa agtccgttcc tccgttgatt gaattattca aaaaacatcc
     5761 acgaaatatt ttattggcac tgggtgcgag gctggcggaa agtgtctcct ctaatattat
     5821 taacgccttt ggtattgtct atatttccag ccaattagca ttgtcgcgag atattcccct
     5881 gacgggtatg ttgattgcct cggcgatcgg tattttcagt tgtccattgg ttggatggtt
     5941 atcagatcgt ataggtcaaa aaagtctgta tttgtcggga gcaggatttt gtgtcctgtt
     6001 cgcgtttcct ttcttcttat tactggatag caaaagtacg ctcatcatct ggtgcagtat
     6061 gatcctcggc tacaacttag gtccgacgat gatgtttgcc gtgcagccaa cacttttcac
     6121 ccgcatgttc ggtaccaaag tacgatatac cggcctttct tttgcttatc aattttccgc
     6181 gattcttggc ggactaagcc cccttatcgc ctcaagtttg ctggcgctag ggggaggaaa
     6241 accgtggtat gtcgctctgt ttttatttgc tgtctcagtt ctttcctttg tttgcgtctg
     6301 gctgattgag ccgaccgatg aacaagaaac cgcttcttac cgctacatca gggaacaatc
     6361 tcatgaaaac tgaacgaaaa aacgagcatt acctggcgct acaacaggcg tttgacgcgc
     6421 catggcctgg gcctgtagga gagttagtga cgctggaaaa aggcaatatt cacctgcaga
     6481 tttatccgca tgatggtgcc aggataacct cgttaaaggc atttggttct gaagtattac
     6541 gccaatggca gccgcagcgc agagcttttc agtatggctg tttcccaatg gtgccgtggg
     6601 cagggcgact ggggaatgcg acgctgaatg ctggtgggca atgttattct ttacccgcca
     6661 ataaaccacc acatgcgttg catggtatgg cttgttactc aacgtgggaa atcatcgaca
     6721 aaacagcaga ttccctgact ttacgtatgc ccctggcttc accgtggccc tggcaagggg
     6781 aagtgattca gacttttttg ctggagaacg atgcgctggt tttgcaactg gaagtccatt
     6841 catatgccga tacattcccg gcctccgcag gctggcatcc gtggtttgcg aaaaagctga
     6901 ctccgcaaaa tacagaatcg ttgcaggtgc tttttgatgc ggactggcaa gaagaagcgg
     6961 gtagtgatga actgccaact ggaaatcgta tctttcctca agccggtccc tgggatgact
     7021 gttttggttt ttataacggg gtgaaggtta agttactgtg gcctggtaaa ctcgcgatga
     7081 ccatgatctc ttctgcaaac agtctggtgg tatttgataa acagcccgat gccacctgta
     7141 ttaacccgct gactcaggca cctaacgcaa taaatctaac gccggaattg gtgacaccag
     7201 ataggccgct ggtcatcgaa actcgttggc aatttacacc tgaatcttaa ccattcatct
     7261 attgcgaaaa agccagtgaa attactctgc tttttcgcaa tcagcatcac aagattaccc
     7321 ttattgccac atagccaaac ccatcctttc ccgctacagt taatttcttg tggcgcgaaa
     7381 ggaggcaaaa atgctctata tctttgattt aggtaatgtg attgtcgata tcgactttaa
     7441 ccgtgtgctg ggagcctgga gcgatttaac gcgtattccg ctggcatcgc ttaagaagag
     7501 ttttcatatg ggggaggcgt ttcatcagca tgagcgtggg gaaattagcg acgaagcgtt
     7561 cgcagaggcg ctgtgtcatg agatggctct accgctaagc tacgagcagt tctctcacgg
     7621 ctggcaggcg gtgtttgttg cgctgcgccc ggaagtgatc gccatcatgc ataaactgcg
     7681 tgagcagggg catcgcgtgg tggtgctttc caataccaac cgcctgcata ccaccttctg
     7741 gccggaagaa tacccggaaa ttcgtgatgc tgctgaccat atctatctgt cgcaagatct
     7801 ggggatgcgc aaacctgaag cacgaattta ccagcatgtt ttgcaggcgg aaggtttttc
     7861 acccagcgat acggtctttt tcgacgataa cgccgataat atagaaggag ccaatcagct
     7921 gggcattacc agcattctgg tgaaagataa aaccaccatc ccggactatt tcgcgaaggt
     7981 gttatgctaa aaaccattca ggacaaagcc aggcaccgta cccgtccact atgggcctgg
     8041 ctaaaactac tctggcaacg tattgatgag gacaacatga caaccctggc aggtaacctt
     8101 gcctatgtgt ctttgctctc attagtgccg ctggttgccg ttgtttttgc gcttttcgcc
     8161 gcttttccca tgttttccga cgtcagcatt cagttacgtc actttatttt tgccaacttt
     8221 ctgcctgcta ctggcgatgt tattcagcgg tatatcgaac aatttgttgc caattccaac
     8281 aagatgaccg ccgttggggc gtgcgggctg atcgtcaccg cgttattgtt gatgtactcc
     8341 atcgatagcg cgttgaatac catctggcgc agtaaacgag cgcgaccaaa aatttactcg
     8401 tttgccgtgt actggatgat cttaacgctg gggccgctgc tggcaggggc cagtctggcg
     8461 atcagttcct atttgttctc cctgcgctgg gcgagcgatc tcaatactgt catcgataac
     8521 gtgctgcgta tttttccgct gctgttgtcg tggatctcct tctggttgct gtacagcatt
     8581 gttcctacca tccgcgtacc taaccgcgac gcgattgtcg gcgcgtttgt cgctgcactc
     8641 ctgttcgaag caggaaagaa aggtttcgcg ctttatatca ccatgttccc gtcatatcag
     8701 ctcatttacg gcgtgctggc ggtgatcccc attctcttcg tttgggtcta ctggacatgg
     8761 tgtatcgtct tgcttggcgc ggaaattact gtcactctcg gggaataccg caaactaaaa
     8821 caagcagctg aacaagaaga agacgacgaa ccatgattgc attaattcaa cgcgtaaccc
     8881 gtgccagcgt caccgtggag ggagaagtga cgggcgaaat tggcgcggga cttttggtgt
     8941 tattgggtgt cgaaaaggat gacgacgaac agaaagcaaa ccgtctgtgc gagcgtgtgc
     9001 tcggctaccg catctttagc gatgccgaag gcaagatgaa tctcaacgtg caacaggcgg
     9061 gcggcagtgt actggtggtt tcccagttta ccctcgccgc agataccgaa cgggggatgc
     9121 gcccaagttt ctccaaaggc gcatcaccgg atcgcgcaga ggcgttatat gactatttcg
     9181 tcgaacgctg ccgccagcaa gagatgaaca cgcaaacagg acgcttcgct gccgatatgc
     9241 aggtatcgct ggtcaatgat ggccccgtga cattctggtt gcaggtatga gcctgcttcc
     9301 agggttgtca cgggaaacaa gagagagtat cgctatgtat caccttcggg ttccacaaac
     9361 agaagaagag ttagagcgtt actatcagtt tcgctgggaa atgttgcgta agcccctgca
     9421 tcaaccaaaa ggttcggaac gcgacgcgtg ggatgcgatg gcgcatcacc agatggtcgt
     9481 cgacgagcag ggtaatctgg tggcggtagg ccgattgtat attaatgccg acaatgaagc
     9541 gtccattcgc tttatggccg ttcacccgga cgtgcaggac aaagggttag gaacgctgat
     9601 ggcgatgacc ctggagtcgg tggcgcgtca ggaaggcgtt aagcgcgtga cctgtagcgc
     9661 ccgtgaagac gcggtggagt ttttcgccaa gctgggattc attaatcagg gggagatcac
     9721 caccccaact accacgccga ttcgccattt tttgatgatt aaacccgttg ccactctgga
     9781 tgacattctg catcgcggtg actggtgcgc gcagctgcaa caggcgtggt acgaacatat
     9841 cccgttaagc gaaaaaatgg gcgtgcgcat tcagcaatat accgggcaaa aatttatcac
     9901 caccatgcca gaaaccggta atcagaatcc gcaccatacg ttgtttgccg ggagtttatt
     9961 ctcgctggcg acgctcaccg gttggggact tatctggctg atgctgcgtg aacgccacct
    10021 cggcggaacg attattctgg cggatgcgca tatccgctac agcaagccga ttagcggtaa
    10081 acctcatgcg gtagccgacc ttggtgcctt aagcggcgat ctcgaccgtc tggcgcgcgg
    10141 acgaaaagca cgggtgcaga tgcaagttga aatctttggc gacgagacgc cgggtgcagt
    10201 gtttgaaggc acgtatatcg ttctgcccgc gaagccattt ggcccgtatg aagagggcgg
    10261 gaacgaagaa gagtagctta tggagagggg cggtgctgcc tctctcattc attccggt
//
LOCUS       DAJVRF010000139        10276 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJVRF010000139 DAJVRF010000000
VERSION     DAJVRF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10276
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.139"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(55..1338)
                     /gene="aroA"
                     /locus_tag="OQN13_003277"
     CDS             complement(55..1338)
                     /gene="aroA"
                     /locus_tag="OQN13_003277"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCS1616556.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1409..2497)
                     /gene="serC"
                     /locus_tag="OQN13_003278"
     CDS             complement(1409..2497)
                     /gene="serC"
                     /locus_tag="OQN13_003278"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCS1616557.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2696..3388)
                     /locus_tag="OQN13_003279"
     CDS             complement(2696..3388)
                     /locus_tag="OQN13_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCS1616558.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3518..5278
                     /locus_tag="OQN13_003280"
     CDS             3518..5278
                     /locus_tag="OQN13_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCS1616559.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5684..6541
                     /gene="focA"
                     /locus_tag="OQN13_003281"
     CDS             5684..6541
                     /gene="focA"
                     /locus_tag="OQN13_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCS1616560.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6596..8878
                     /gene="pflB"
                     /locus_tag="OQN13_003282"
     CDS             6596..8878
                     /gene="pflB"
                     /locus_tag="OQN13_003282"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCS1616561.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9070..9810
                     /gene="pflA"
                     /locus_tag="OQN13_003283"
     CDS             9070..9810
                     /gene="pflA"
                     /locus_tag="OQN13_003283"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCS1616562.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(9903..10079)
                     /locus_tag="OQN13_003284"
     CDS             complement(9903..10079)
                     /locus_tag="OQN13_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005043118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCS1616563.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNIISRYYLRYGIW"
     gene            10179..>10276
                     /locus_tag="OQN13_003285"
     CDS             10179..>10276
                     /locus_tag="OQN13_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1616564.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSA"
BASE COUNT         2633 a         2580 c         2445 g         2618 t
ORIGIN      
        1 atgtgccgac gttttgataa agaaagattt ggctatttat tgcccgttgt tcattcaggc
       61 tgcctggcta atccgcgcca actgctcgaa ataatccgga aatgttttgg ccgtacattt
      121 gggatcaaga atcgtcactg gtgtatctga caacgccacc agcgagaaac acatcgccat
      181 ccggtgatca ttgtatgtcg cgatctcggc aaagttcagt ttttccggtg gagtgatacg
      241 aatgtaatcg tgcccctctt ctacttccgc accgacttta cgcagttctg ttgccatcgc
      301 aaacaggcga tcggtctctt taacacgcca gttatagata ttgcgcagcg tggtggtgcc
      361 ttttgcaaat aacgccgccg tggcaatggt catcgccgca tcgggaatat ggttcatatc
      421 catatcaata gcgttcagtt caccacgcgt gcaggaaata taatcatcgc cccagcaaat
      481 ggtcgcgccc attttttcca gcacatcagc aaagcgaata tcaccctgca tactgttacg
      541 tccaataccg gtcactttta cagtgccgcc tttgattgct gctgctgcca gaaagtaaga
      601 agccgaagat gcatcgcctt cgaccaaata agtacccgga gactgataag actgcccgcc
      661 ttttacgaca aattgttgat agtgctgatt ttcaatttca acaccaaacg tcttcatcag
      721 attgagtgtg atgtcgatat aaggtttaga aaccagatcg cctttaatac gaatcaccgt
      781 atcttccggc gcaagaggcg cagtcattaa cagtgcggtg aggaattggc tggaaacgga
      841 gccatcaacg tcaacgttgc cgccagtaaa gccgccctgt aaacgcaacg gcggataatt
      901 ttcttgttcc aggtaagtga tcttcgcccc gcccaggcgc agcgcatcca ccagatgacc
      961 aatcgggcgt tctttcatac gcggctcacc ggtcagcaca atatcattgc tacccagaca
     1021 aagagctgcc gccagcggac gcattgccgt tccggcgtta ccgaggaaca actccagggc
     1081 accttctgcg tgtaatggac cgccgttacc gataatttcg caacgcgtac gatcggctga
     1141 aagcgtatag cttaccccta acgctgttaa tgcattcagc atatggcgca cgtcatcgct
     1201 atccagcaga ttggttaata ctgttttgcc gtgtgctaat gccgccagca ataaagcgcg
     1261 gttagaaacg ctcttggaac cgggcagatt aatagtgcca tcgacacgag cgatgggttg
     1321 taacgtcagg gattccatga actcaactct ctacaacaga aataaaaacc ccacaggctg
     1381 gctgtgggga ttaagcaaaa tttcggcatt aaccgtgacg gcgttcgaac tcaaccatga
     1441 agtctgtcag cgctttaacg ccttccagcg gcatggcgtt ataaatagaa gcgcgcattc
     1501 cgccgaccac acggtgacct ttcagtgcat gaaggccagc agcaaaagac tcttcaagga
     1561 acaatttgtc aagcgcactg tccgccaact ggaacggcac gttcatccgc gaacggttag
     1621 ctttcgccac gtcattgcgg tagaaatcgc tgttatcaat caccccatat agcagttctg
     1681 ctttttgctg attgatttta tccatttcag ctacaccgcc gttcgctttc agccatttaa
     1741 agaccagacc agatagatac caggcaaatg tcggcggcgt gttaaacatg gagccgttat
     1801 cgttgaggat ggaataatcc agaatcgacg gacacgcgat attcgctttg cccagcaaat
     1861 cttcacgaac gatgacgatt gtcaggccag ccgggccgat atttttctgc gcgccagcgt
     1921 aaatcacgcc ataacggctg acgtcaatcg gacgggaaag aatggttgaa gagaagtcgg
     1981 cggcgaccac cacatctttg ccgaagtctg gcgtttcgtc gatggcgata ccgtcgatgg
     2041 tttcattcgg gcagtaatgc atataagcag cattatcaga gagttgccat tcacgcattg
     2101 gcttaaccgc gcgcagacca tcaacagtca ctttggcgtc aaagacatta ggcgtgcagt
     2161 attttttcgc ttctttaatg gcacttgccg cccagtaacc ggcatcaaca taatctgcgg
     2221 tggttttatc accgagaata ttcagcggta ccgcagcaaa ctgaccgcga ccaccgccat
     2281 ggcagaataa taccttgtag ttggagggga cattaagaag atcgcgaaaa tccttctcgg
     2341 cttcctctgc aacctgaatg aactctttgc cacggtgact cacttccatc accgacgtac
     2401 caagaccgtt ccagtcgcgc agttcctgtt gagcctgttt aagcacctct gccggtagca
     2461 ttgccggacc agaactaaaa ttgaagattt gagccattgc ccctcaccac gttgcgttgc
     2521 tattaacccg cggtcaatcg attgcgacca caggtaatac attattcctg tggctatcgg
     2581 ttttatcatt cagtgacaca catcgcaatg agtaaaaccg ttgagggcaa aaatgtggct
     2641 tgcatcacac aaaagaatct ctcggattat tagcaaaaag ccgatgtttt tactgtcagg
     2701 tcacccgttt tgcccgactt gccttcgtcc attctggatt tgcacagcgc ggacataatt
     2761 gtttttcccc cgctttcata tgaatgattt cactacaacg aatacaggca tagtccgccg
     2821 actccggcac cactttgtaa cgctgcgtac aatcactggg taaaataagt aaacccggtt
     2881 tcaccttgtc atcttcaaag aaatagacac tgatttgccc gttggtttcg agaatcgcca
     2941 gacgtacctg ccccagctgc tccacgccac gcaatcgtag ctccataaag aactcaaatt
     3001 ccgtcatgtt ggagttattg agtttcgacc aggccagctc gccatcttca ataataacaa
     3061 ccggcttacc ttccagtaga tcttccagtt tttcactgtg cgccatcaac cacattacca
     3121 ggcggtataa caacgccaga gtgataaaga cgataagtac cggcaccatc ggcacatcat
     3181 cataaaacgc cacatctccc gccgctgaac ccagcgtgag aatgattaac acttcaaaca
     3241 gcgacatctg tcgcacaccg cgtcttccgg tcattttgag gaacaaaaaa accagtacaa
     3301 aggtataaag actacgtagt gcaacttcgc caaggaaatc aaaaggcact ttatcaaatg
     3361 ccatacggtg gagatcgaac gccttcattt ttatgctctt atcgtggttt atccgctaaa
     3421 agcatagtca acagttcatc ggcatggcgg cggtgatagc gcctttgaga taaaacccgt
     3481 attattgcgc gctttccgta cgactaaagt gattttcatg acgcaaacat ttatccccgg
     3541 caaagatgcc gctctggaag attccatcgc tcgcttccag caaaaacttt cagacctcgg
     3601 ctttcagatt gaagaggcct cctggctgaa tcctgtgcct aacgtctggt ctgtacatat
     3661 tcgcgacaaa gagtgcgcac tgtgttttac caacggtaaa ggcgcaacca aaaaagcggc
     3721 gctggcttct gcacttggtg aatatttcga gcgtctctca accaactact tttttgctga
     3781 tttctggctg ggcgaaacca tcgccaacgg tccgttcgtg cattatccca acgaaaaatg
     3841 gttcccactg accgaaaatg acgatgtgcc agaaggacta ctcgatgacc gtctgcgcgc
     3901 gttttatgat ccggagaatg aactgaccgg cagcatgctg attgacctac aatccggtaa
     3961 cgaagatcgt ggtatttgcg gcctgccgtt tacgcgtcag tccgacaatc agaccgttta
     4021 tattccgatg aatatcattg gtaacctgta cgtctccaac ggtatgtccg caggtaatac
     4081 ccgcaacgaa gcacgcgttc agggattgtc tgaagttttc gaacgctacg tgaaaaaccg
     4141 cattattgct gaaagcatca gcctgccaga gatcccggca gacgtgctgg cgcgttaccc
     4201 agcagtagtt gaagccatcg aaacgctgga agcggagggt ttcccaatct tcgcttatga
     4261 cggttcgctt ggcggccagt atccggtgat ttgcgtggta ctgttcaatc ctgctaacgg
     4321 tacctgcttt gcctctttcg gtgcgcatcc tgattttggc gtagcactgg aacgtaccgt
     4381 gaccgagctg ctgcaaggtc gtggcctgaa agatttggat gtgtttactc cgccaacctt
     4441 cgatgatgaa gaagtcgctg aacataccaa cctcgaaacg cactttatcg attccagcgg
     4501 tttaatctcc tgggacctgt tcaagcagga tgccgattat ccgtttgtgg actggaattt
     4561 ctccggcacc acggaagaag agtttgctac gctgatggct atcttcaaca aagaagataa
     4621 agaagtttat attgccgatt acgagcatct gggcgtttat gcttgccgta ttatcgtgcc
     4681 tggcatgtcc gatatttatc cggctgaaga tctgtggctc gcgaataaca gtatgggcag
     4741 ccatttacgt gaaacgattc tttcgctacc aggcagcgag tgggaaaaag aagattacct
     4801 gaacctcatc gagcaactgg atgaagaagg ttttgatgac tttacccgcg tgcgtgagct
     4861 gttgggtctg gcgaccgggt cggataacgg ttggtacacc ctgcgtatcg gtgaattaaa
     4921 agccatgctg gcgctggctg gtggcgatct ggaacaggct ctggtctgga ccgaatggac
     4981 gatggagttt aactcatcag tatttagtcc ggaacgcgcc aactattatc gctgcctgca
     5041 aacgttgtta ttactggcac aggaagaaga tcgccagccg ctgcaatatc tgaatgcgtt
     5101 tgttcgcatg tacggcgcag atgccgtaga agccgccagt gcggcaatga gcggcgaagc
     5161 ggcgttttac ggcctgcaac cggtagatag cgatctgcac gcgtttgctg cacatcagtc
     5221 gttgttgaag gcctacgaaa agctgcagcg cgccaaagca gcattctggg caaaataaaa
     5281 tcaaatagcc tacgcaatgt aggcttaatg attagtctga gttatattac ggggcgtttt
     5341 tttaatgccc cgctttacat atatttgcat taataaaata attgtaatta taaggttaaa
     5401 tatcggtaat ttgtatttaa taaatacgat cgatattgtt actttattcg cctgatgctc
     5461 ccttttaatt aactgtttta gcggaggatg cggaaaaaat tcaactcatt tgttaatttt
     5521 taaaatttat ttttatttgg ataatcaaat gtttactccg tatctgcata aaaaccatgc
     5581 gagttacggg cctataagcc aggcgagata tgatctatat caatttctca tctataatgc
     5641 tttgttagta tctcgtcgcc gacttaataa agagagagtt agtgtgaaag ctgacaaccc
     5701 ttttgatctt ttacttcctg ctgcaatggc caaagtggcc gaagaggcgg gtgtctataa
     5761 agcaacgaaa catccgctta agactttcta tctggcgatt accgccggtg ttttcatctc
     5821 aatcgcattc gtcttctata tcacagcaac cactggcaca ggcacaatgc ccttcggcat
     5881 ggcaaaactg gttggcggca tttgcttctc tctggggctg attctttgtg ttgtctgcgg
     5941 agccgatctc tttacttcca ccgtgttgat tgttgttgct aaggcgagtg ggcgcatcac
     6001 ctggggtcag ttggcgaaaa actggctaaa tgtctatttt ggcaacctgg tcggcgcact
     6061 gctgtttgta cttttaatgt ggctttccgg cgagtatatg accgcaaatg gtcaatgggg
     6121 actaaacgtc ctacaaaccg ccgaccacaa agtgcaccat acttttattg aggccgtctg
     6181 tcttggtatc ctggcaaacc tgatggtatg tctggcagta tggatgagtt attctggccg
     6241 cagcctgatg gacaaagcgt tcattatggt gctgccggtc gcgatgtttg ttgccagcgg
     6301 ttttgagcac agtatcgcaa acatgtttat gatcccgatg ggtattgtaa tccgcgactt
     6361 cgcatccccg gaattttgga ccgcagtcgg ttctgcaccg gaaaattttt ctcacctgac
     6421 cgtgatgaat ttcatcactg ataacctgat tccggttacg atcggcaaca ttatcggtgg
     6481 tggtttgttg gttgggttga catactgggt catttacctg cgtgaaaacg accaccatta
     6541 atggttgtcg aagtacgcag taaataaaaa atccacttaa gaaggtaggt gttacatgtc
     6601 cgagcttaat gaaaagttag ccacagcctg ggaaggtttt accaaaggtg actggcagaa
     6661 tgaagtaaac gtccgtgact tcattcagaa aaactacact ccgtacgagg gtgacgagtc
     6721 cttcctggct ggcgctactg aagcgaccac caccctgtgg gacaaagtaa tggaaggcgt
     6781 taaactggaa aaccgcactc acgcgccagt tgactttgac accgctgttg cttccaccat
     6841 cacctctcac gacgctggct acatcaacaa gcagcttgag aaaatcgttg gtctgcagac
     6901 tgaagctccg ctgaaacgtg ctcttatccc gttcggtggt atcaaaatga tcgaaggttc
     6961 ctgcaaagcg tacaaccgcg aactggatcc gatgatcaaa aaaatcttca ctgaataccg
     7021 taaaactcac aaccagggcg tgttcgacgt ttacactccg gacatcctgc gttgccgtaa
     7081 atctggtgtt ctgaccggtc tgccagatgc atatggccgt ggccgtatca tcggtgacta
     7141 ccgtcgcgtt gcgctgtacg gtatcgacta cctgatgaaa gacaaactgg cacagttcac
     7201 ttctctgcag gctgatctgg aaaacggcgt aaacttggaa cagactatcc gtctgcgcga
     7261 agaaatcgct gaacagcacc gcgctctggg tcagatgaaa gaaatggctg cgaaatacgg
     7321 ctacgacatc tctggtccgg ctaccaacgc tcaggaagct atccagtgga cttacttcgg
     7381 ctacctggct gctgttaagt ctcagaacgg tgctgcaatg tccttcggtc gtacctccac
     7441 cttcctggat gtgtacatcg aacgtgacct gaaagctggc aagatcaccg aacaagaagc
     7501 gcaggaaatg gttgaccacc tggtcatgaa actgcgtatg gttcgcttcc tgcgtactcc
     7561 ggaatacgat gaactgttct ctggcgaccc gatctgggca accgaatcta tcggtggtat
     7621 gggcctcgac ggtcgtaccc tggttaccaa aaacagcttc cgtttcctga acaccctgta
     7681 caccatgggt ccgtctccgg aaccgaacat gaccattctg tggtctgaaa aactgccgct
     7741 gaacttcaag aaattcgccg ctaaagtgtc catcgacacc tcttctctgc agtatgagaa
     7801 cgatgacctg atgcgtccgg acttcaacaa cgatgactac gctattgctt gctgcgtaag
     7861 cccgatgatc gttggtaaac aaatgcagtt cttcggtgcg cgtgcaaacc tggcgaaaac
     7921 catgctgtac gcaatcaacg gcggcgttga cgaaaaactg aaaatgcagg ttggtccgaa
     7981 gtctgaaccg atcaaaggcg atgtcctgaa ctatgatgaa gtgatggagc gcatggatca
     8041 cttcatggac tggctggcta aacagtacat cactgcacta aacatcatcc actacatgca
     8101 cgacaagtac agctacgaag cctctctgat ggcgctgcac gaccgtgacg ttatccgcac
     8161 catggcgtgt ggtatcgctg gtctgtccgt tgctgctgac tccctgtctg caatcaaata
     8221 tgcgaaagtt aaaccgattc gtgacgaaga cggtctggct atcgacttcg aaatcgaagg
     8281 cgaatacccg cagtttggta acaacgatcc gcgtgtagat gacctggctg ttgacctggt
     8341 agaacgtttc atgaagaaaa ttcagaaact gcacacctac cgtgacgcta tcccgactca
     8401 gtctgttctg accatcactt ctaacgttgt gtatggtaag aaaacgggta acaccccaga
     8461 cggtcgtcgt gctggcgcgc cgttcggacc gggtgctaac ccgatgcacg gtcgtgacca
     8521 gaaaggtgca gtagcctctc tgacttccgt tgctaaactg ccgtttgctt acgctaaaga
     8581 tggtatctcc tacaccttct ctatcgttcc gaacgcactg ggtaaagacg acgaagttcg
     8641 taagaccaac ctggctggtc tgatggatgg ttacttccac cacgaagcat ccatcgaagg
     8701 tggtcagcac ctgaacgtta acgtgatgaa ccgtgaaatg ctgctcgacg cgatggaaaa
     8761 cccggaaaaa tatccgcagc tgaccatccg tgtatctggc tacgcagtac gtttcaactc
     8821 gctgactaaa gaacagcagc aggacgttat tactcgtacc ttcactcaat ctatgtaatt
     8881 agatttgact gaaatcgtac agtaaaaagc gtacaataaa ggctccacga aagtggggcc
     8941 ttttttagca cgagagcctt ttttgtcagc tatctatact ctaaggtgac tgccaaaaca
     9001 gactcgacgt agccttcgag ctgcgcacca acacggcctc agatgggcca catctggaga
     9061 aacaccgcaa tgtcagttat tggtcgcatt cactcctttg aatcctgtgg aaccgtagac
     9121 ggcccgggta ttcgctttat cacctttttc cagggctgcc tgatgcgctg cctgtattgt
     9181 cataaccgcg acacctggga tacgcatggc ggtaaagaag ttaccgttga agatttaatg
     9241 aaagaggtgg tgacctatcg ccactttatg aacgcttccg gcggcggcgt taccgcatcc
     9301 ggcggtgagg caatcctaca agctgagttt gttcgtgact ggttccgcgc ctgcaaaaaa
     9361 gaaggcattc atacctgtct ggacaccaac ggttttgttc gtcgttacga tccggtgatt
     9421 gatgaactgc tggaagtaac cgacctggta atgctcgatc tcaaacagat gaacgacgag
     9481 atccaccaaa atctggttgg agtttccaat caccgcacgc tggagttcgc caaatatctg
     9541 gcgaacaaaa atgtgaaggt gtggatccgc tacgttgttg tcccaggctg gtctgacgat
     9601 gacgattcag cgcatcgcct cggtgaattt acccgtgata tgggcaacgt tgagaaaatc
     9661 gagcttctcc cctaccacga gctgggcaaa cacaaatggg tggcaatggg tgaagagtac
     9721 aaactcgacg gtgttaaacc accgaagaaa gagaccatgg aacgcgtgaa aggcattctt
     9781 gagcagtacg gtcataaggt aatgttctaa tttccccgta aagcggcaac tttattgagt
     9841 tgccgctttt ttattccgca cttcattctg ctacagcgtt tttaacacat cttatagtgc
     9901 atctaccata tcccgtacct gagataataa cgactgataa tattacgccg atataaatcg
     9961 agttccgtca cctgtatttt ccgctccatt gcccgctgat ggatcgcatc agcaatatgt
    10021 gcggtcaatg aatcatgacg aggatgggcc cataccaaat aaatacgttc agactgcata
    10081 attttacctc cacgttaagt tctggagcgc actttacgca ggcggcataa taatgtaaac
    10141 gtgatgattc aggatgagtt aatgaaggat tttcactaat gcggatgaat atgtctgact
    10201 ttgccacttt ctttgctgtg gcccgtaatc aaagctttcg tgcagcgggc gatgagttag
    10261 gcttatcctc gtccgc
//
LOCUS       DAJVRF010000140         9852 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJVRF010000140 DAJVRF010000000
VERSION     DAJVRF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9852
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.140"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(382..1359)
                     /gene="epmA"
                     /locus_tag="OQN13_003286"
     CDS             complement(382..1359)
                     /gene="epmA"
                     /locus_tag="OQN13_003286"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCS1616565.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1684..3492
                     /gene="frdA"
                     /locus_tag="OQN13_003287"
     CDS             1684..3492
                     /gene="frdA"
                     /locus_tag="OQN13_003287"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCS1616566.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3485..4219
                     /gene="frdB"
                     /locus_tag="OQN13_003288"
     CDS             3485..4219
                     /gene="frdB"
                     /locus_tag="OQN13_003288"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCS1616567.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4230..4625
                     /gene="frdC"
                     /locus_tag="OQN13_003289"
     CDS             4230..4625
                     /gene="frdC"
                     /locus_tag="OQN13_003289"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCS1616568.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4636..4995
                     /gene="frdD"
                     /locus_tag="OQN13_003290"
     CDS             4636..4995
                     /gene="frdD"
                     /locus_tag="OQN13_003290"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS1616569.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5058..6191
                     /locus_tag="OQN13_003291"
     CDS             5058..6191
                     /locus_tag="OQN13_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS1616570.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6280..6813
                     /gene="blc"
                     /locus_tag="OQN13_003292"
     CDS             6280..6813
                     /gene="blc"
                     /locus_tag="OQN13_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCS1616571.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="OQN13_003293"
     CDS             complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="OQN13_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCS1616572.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="OQN13_003294"
     CDS             complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="OQN13_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCS1616573.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="OQN13_003295"
     CDS             complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="OQN13_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCS1616574.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7736..8302)
                     /gene="efp"
                     /locus_tag="OQN13_003296"
     CDS             complement(7736..8302)
                     /gene="efp"
                     /locus_tag="OQN13_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCS1616575.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8344..9372
                     /gene="epmB"
                     /locus_tag="OQN13_003297"
     CDS             8344..9372
                     /gene="epmB"
                     /locus_tag="OQN13_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCS1616576.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9762..>9852
                     /locus_tag="OQN13_003298"
     CDS             9762..>9852
                     /locus_tag="OQN13_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616577.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPR"
BASE COUNT         2518 a         2505 c         2502 g         2327 t
ORIGIN      
        1 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
       61 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
      121 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      181 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta
      241 aaaaccaata cagaattgat acccggatga tatcaaatat ccagtcttaa attgtggcct
      301 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      361 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      421 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
      481 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      541 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      601 cgttaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      661 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      721 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      781 ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg ttactcaaat ccagtttcgc
      841 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
      901 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
      961 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
     1021 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1081 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     1141 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1201 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1261 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcacgtttta ataagttagg
     1321 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1381 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1441 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1501 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1561 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1621 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1681 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1741 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1801 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1861 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1921 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     1981 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2041 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2101 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2161 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2221 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2281 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2341 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2401 tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat
     2461 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2521 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2581 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
     2641 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2701 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2761 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2821 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
     2881 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2941 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3001 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     3061 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3121 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     3181 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3241 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3301 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3361 ggcacgactc gcctagagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3421 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3481 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3541 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3601 tgcgctggtc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     3661 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3721 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3781 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3841 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3901 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3961 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4021 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4081 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     4141 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4201 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4261 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4321 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4381 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4441 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     4501 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4561 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4621 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4681 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4741 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     4801 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4861 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     4921 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4981 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     5041 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     5101 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5161 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     5221 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5281 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5341 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttactgcta
     5401 aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat
     5461 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     5521 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     5581 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
     5641 tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt
     5701 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     5761 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     5821 ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct
     5881 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
     5941 atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa
     6001 aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     6061 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     6121 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
     6181 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     6241 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6301 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     6361 gtgacagtag tgaataattt cgacgctaaa cgctatctcg gtacctggta tgagattgcc
     6421 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagtctgcgt
     6481 gatgacggcg gcctgaatgt catcaataaa ggctataacc ctgacagaga aatgtggcag
     6541 cagagtgaag ggaaagcgta ctttaccggc gcaccaacac gcgctgcgct gaaagtgtca
     6601 ttctttggtc ctttctatgg cggttataac gtcattgcac ttgatcggga ataccgccat
     6661 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     6721 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     6781 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     6841 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     6901 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     6961 cctaccggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
     7021 acagtaataa cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     7081 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     7141 gaattggggc catccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     7201 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
     7261 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt
     7321 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     7381 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     7441 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
     7501 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
     7561 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
     7621 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
     7681 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
     7741 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
     7801 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
     7861 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
     7921 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
     7981 gcagccattt agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
     8041 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
     8101 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
     8161 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
     8221 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
     8281 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
     8341 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
     8401 gccgatgttg tgaccgatcc tggtgaactt ctgcgtcttt tgaatataga cgcggacgaa
     8461 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
     8521 atcgatcgca tggagaaagg caatccggac gatcctattt tgcgtcaggt acttacctcg
     8581 caagatgagt ttgtcgtcgc gtccggattc tccaccgacc cactggaaga acagcacagc
     8641 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
     8701 tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
     8761 aacaagcgta actggcagac tgcacttgag tatgttgctg cgcatccgga actggacgag
     8821 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
     8881 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
     8941 gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa
     9001 atcttgctgg tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg
     9061 atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
     9121 gtgaacgata acgcacaaac gttggcaaac ctgagtaatg cgttgttcga tgccggcgtg
     9181 atgccgtatt acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt
     9241 gatgacgaag cacggcagat tatgcgtgag ttgctgacat tggtgtcggg atatctggtg
     9301 ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta
     9361 cgccagcagt aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc
     9421 aggcctacat tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
     9481 gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg
     9541 gaaatttaat ctttgcccgt tgcataacct ttaattattc atatacattg ctgttgttac
     9601 tctttatata gccaacacca ataattaacc tttcttttct gtttatatag agtgagtacg
     9661 gctatttatg ttattttaac aactattact accaagccca tttatctggc ttataaatta
     9721 aaattaatta tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg
     9781 gtgttaatac cggtgttatt cgcaagagta ataatttcat tgcactggcg ctgaaaatta
     9841 aagaacctcg ca
//
LOCUS       DAJVRF010000141         9542 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJVRF010000141 DAJVRF010000000
VERSION     DAJVRF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.141"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..1321
                     /locus_tag="OQN13_003299"
     CDS             <1..1321
                     /locus_tag="OQN13_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1616578.1"
                     /translation="PQFRIPAELIQHDIDFVAAHGVKFEYGCSPDLTVEQLKNQGFHY
                     VLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVVVVGAGNTAMDCAR
                     AALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNPERFDADGTLTLRV
                     MSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMGVPLDKNGWPDVDH
                     NGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSHQNDKYWNNVNPAE
                     IYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPNRANVSIAVPGFQN
                     RFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSSTPGFLVEDCRVRV
                     RLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRVEV"
     gene            1324..2652
                     /gene="ssnA"
                     /locus_tag="OQN13_003300"
     CDS             1324..2652
                     /gene="ssnA"
                     /locus_tag="OQN13_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HCS1616579.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            2703..3482
                     /locus_tag="OQN13_003301"
     CDS             2703..3482
                     /locus_tag="OQN13_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HCS1616580.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            3479..6418
                     /locus_tag="OQN13_003302"
     CDS             3479..6418
                     /locus_tag="OQN13_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HCS1616581.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDTCGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            6351..6665
                     /locus_tag="OQN13_003303"
     CDS             6351..6665
                     /locus_tag="OQN13_003303"
                     /inference="COORDINATES: protein motif:HMM:NF019581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCS1616582.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            6701..7051
                     /gene="ghxQ"
                     /locus_tag="OQN13_003304"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             6701..7051
                     /gene="ghxQ"
                     /locus_tag="OQN13_003304"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(7120..7608)
                     /locus_tag="OQN13_003305"
     CDS             complement(7120..7608)
                     /locus_tag="OQN13_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCS1616583.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(7608..9527)
                     /gene="ygfT"
                     /locus_tag="OQN13_003306"
     CDS             complement(7608..9527)
                     /gene="ygfT"
                     /locus_tag="OQN13_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HCS1616584.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
BASE COUNT         2301 a         2559 c         2488 g         2194 t
ORIGIN      
        1 tcctcagttc cgtattcctg cagagttaat tcagcacgat atcgattttg ttgccgctca
       61 cggcgtgaaa tttgagtatg gctgctcacc cgatttaacc gttgaacagt taaaaaatca
      121 gggcttccac tatgttctga ttgccaccgg cactgataaa aatagcggtg tgaaactggc
      181 gggcgacaac caaaatgtct ggaaatcact ccccttcctg cgtgaataca acaagggtac
      241 agcgctcaag ctgggcaaac atgtggtcgt tgtcggggcg ggtaacaccg caatggactg
      301 cgctcgtgcg gcgttacgcg ttccaggcgt agaaaaagca acgatcgttt accgtcgttc
      361 actacaagag atgcccgcat ggcgcgaaga gtatgaagaa gcgttgcacg acggcgtaga
      421 gttccgtttc ctgaataatc cggaacgttt cgatgctgat ggcaccttaa ccttgcgcgt
      481 tatgtcgctt ggcgaaccgg atgagaaagg tcgtcgccgt ccggttgaaa ccaacgaaac
      541 agtaacactg catgtagaca gcctgatcac cgccattggt gaacagcagg atactgaagc
      601 cctgaatgcg atgggcgtgc cgctggacaa aaacggctgg ccagacgtcg accataatgg
      661 cgaaactcgt ctgactgacg tctttatgat cggcgacgta cagcgcggac catcctccat
      721 tgtcgctgct gtcggaaccg cgcgtcgggc gaccgatgcc atccttagtc gggaaaatat
      781 ccgttcccac cagaacgata aatactggaa caacgtcaat ccagcggaaa tctatcaacg
      841 taaaggcgat atctctatca cgctggtgaa cagtgacgat cgtgacgcgt ttgtcgccca
      901 ggaagccgct cgctgcctcg aatgtaacta cgtttgcaac aagtgtgtgg atgtctgccc
      961 gaaccgcgcc aacgtctcca ttgcagtccc tggcttccag aatagattcc agacgctgca
     1021 cctcgacgct tactgtaacg aatgcggcaa ctgcgctcag ttctgtccgt ggaacggtaa
     1081 accatacgaa gacaaaatca ccgtcttcag cctggcgcaa gactttgata acagcagcac
     1141 cccaggcttc cttgtggaag attgccgggt acgagtacgt ctgaataacc aaagctgggt
     1201 gttaaacatc gacagcgaag gtcagtttaa caacgtacca ccggagctga acgatatgtg
     1261 ccgcatcatc agccatgtcc accagcatca tcattatctg ctgggccgcg tggaggtgta
     1321 atcatgttga ttctgaagaa tgtcaccgca gtgcagttac acccggcgaa agtgcaggaa
     1381 ggcgttgata tcgctatcga aaatgacgtg attgtcgcta tcggcgatgc cctgacgcaa
     1441 cgctaccccg atgccagcta caaagagatg catggtcgga ttgtgatgcc gggaattgtc
     1501 tgctcgcaca accattttta ctcggagctt tcccgcggaa ttatggcaaa catcgcccct
     1561 tgcccggatt tcatctcaac gctgaaaaat ctctggtggc ggctcgatcg cgcccttgat
     1621 gaagagtcgc tctattacag cggactgatt tgttccctgg aagcgattaa gagcggatgt
     1681 acatcggtta tcgatcacca tgcctcgccg gcgtatatcg gcgggtcgct ctccacattg
     1741 cgcgacgcat ttttaaaagt tggcctgcgc gcgatgacct gttttgaaac tactgaccgt
     1801 aacaacggca tcaaagagtt gcaggaaggt gtagaagaaa acatccgttt cgcccgtttg
     1861 attgatgagg cgaagaaagc gacaagcgag ccgtatctgg tggaagcaca tatcggtgct
     1921 cacgcgccgt ttaccgtgcc ggatgccggt ctggagatgc tgcgtgaagc cgtgaaagcc
     1981 acaggccgtg gtttgcatat tcacgctgcg gaagaccttt acgacgtttc ctacagtcac
     2041 cactggtacg gcaaagacct gctggcacga ctggcgcaat tcgacctcat cgacagcaaa
     2101 acgctggtcg ctcatgggct gtacttgtcg aaagatgaca tcgccctact caatcagcgc
     2161 gatgcgttcc tggtgcataa cgcccgttca aacatgaaca accatgtcgg ctacaaccat
     2221 caccttagcg acatccgcaa cctggcattg ggaacggacg gcattggttc ggacatgttt
     2281 gaagagatga aatttgcctt ctttaaacat cgcgatgcgg gtggtccgct gtggcctgac
     2341 agttttgcca aagccctgac taacggtaac gaactgatga gccgcaactt tggcgcgaaa
     2401 tttgggcttc tggaagccgg ttacaaagct gatttgacca tttgcgatta caactcgccg
     2461 acgccgctgc tggcagacaa tatcgccggg catatcgctt tcggtatggg ctcaggcagc
     2521 gttcacagcg tgatggtcaa tggtgtgatg gtctatgaag accgtcagtt taacttcgat
     2581 tgcgattcca tttatgcaca agccagaaaa gccgctgcca gtatgtggcg tcggatggat
     2641 gcgctggcat aaatgacaga tgccctcttc ccgcagggaa gagggttaaa taaggaaaga
     2701 agatgattga acaatttttc aggcccgact ctgtcgaaca ggcgctggaa ctgaagcgcc
     2761 gctaccagga tgaagccgtc tggttcgccg ggggcagcaa actcaatgct acaccaaccc
     2821 gtaccgataa aaagattgcc atttccttac aggatctgga actggactgg gttgactggg
     2881 ataacggtgc actgcggatt ggtgcaatgt ctcgcttgca gccactgcgt gatgcgcgct
     2941 ttattcctgc agcactgcgt gaagccctcg gttttgttta ctcacgccat gttcgtaatc
     3001 agtcgaccat tggtggtgaa atcgccgccc ggcaggaaga gtcagtgctg cttcccgtcc
     3061 tgctagcact ggatgctgaa ctggtttttg gcaacggcga aacgctgtca atcgaggact
     3121 acctggcctg cccatgcgat cgcctgctaa ccgaaattat cattaaagat ccgtatcgca
     3181 cctgtgcgac ccgcaaaatt agccgttctc aggcaggttt aaccgtcgtg acggcagccg
     3241 ttgcaatgac agaccacgac ggtatgcgaa ttgcgctgga tggcgtcgcc agtaaagcac
     3301 tgcgtctgca cgatgtcgaa aaacaaaatc tggaaggcaa tgcacttgaa caggctgtcg
     3361 ccaacgccat tttcccgcag gaagatttgc ggggcagcgt ggcctataaa cgctatatca
     3421 cgggagttct ggtagccgac ctgtatgccg actgccaaca ggctggggag gaagccgtat
     3481 gatcatccac tttactttaa atggcgcgcc tcaggagcta accgttaatc caggcgaaaa
     3541 cgtgcaaaag ctgttgttta acatgggaat gcactctgta cgcaacagtg atgatggctt
     3601 cgggtttgcc ggttctgacg caataatctt taacggtaat atcgttaacg cgtccttgct
     3661 tattgccgca cagttagaga aggcagatat tcgtaccgca gaatctctgg gtaaatggaa
     3721 cgagttaagt ctggttcaac aggcaatggt tgatgttggc gtggtgcagt ctggttataa
     3781 cgatccagct gcagctctga ttatcaccga tcttctcgat cgcatcgccg cacctacccg
     3841 cgaagagatc gacgacgcgc tttctggttt gttcagccgc gatgcaggct ggcaacaata
     3901 ctatcaggtc attgaactgg cggttgcacg taaaaataat ccgcaggcca ccattgatat
     3961 cgctccgact ttccgtgacg accttgacgt cattggcaag cattatccta aaactgatgc
     4021 cgcgaaaatg gtgcaggcga aaccctgcta tgttgaagac tgcgtaacgg ctgacgcctg
     4081 cgtcattaaa atgttacgta gcccacacgc tcacgcactg attactcatc tggatgtcag
     4141 caaagctgaa gccttaccgg gcgtcgttca cgttattact cacctgaatt gccctgatat
     4201 ctactatacc ccggggggtc agagcgcacc ggaaccgtca ccgcttgacc gccgtatgtt
     4261 cggcaagaaa atgcgtcacg tcggcgatcg tgttgctgcg gtcgtcgcag aaagtgaaga
     4321 aattgcgctc gaagcactga agctcatcga cgttgaatat gaagtgctta agccggtaat
     4381 gtcgatcgac gaagcaatgg cggaagatgc gcctgtcgtg cacgatgaac cggtggtgta
     4441 tgttgctggt gcgccagata ctctggaaga cgataacagc catgcagccc agcgcggcga
     4501 gcatatgatc atcaacttcc cgatcggttc tcgccctcgc aaaaatatcg ccgccagtat
     4561 tcatggtcat attggcgata tggacaaagg ctttgccgat gccgatgtga tcattgagcg
     4621 aacctataac tcaacgcagg cacagcagtg cccgactgaa acacatatct gctttacccg
     4681 gatggacggc gatcgtctgg ttatccacgc ctccacccag gtaccatggc acttacgccg
     4741 ccaggtcgcg cgcctcgtgg gcatgaaaca gcataaagtt catgtcatta aagagcgagt
     4801 tggcggcggt tttggttcca aacaggacat cctgctggaa gaagtgtgcg cctgggcaac
     4861 ctgcgtgacc gggcgtccgg tactattccg ctacacccgt gaagaagagt ttattgctaa
     4921 cacctctcgt cacgtcgcga aagtcaccgt caaactggga gcgaaaaaag atggtcgcct
     4981 gacggcagtg aagatggatt tccgcgccaa cactggccct tacggcaacc actcactcac
     5041 cgtaccgtgt aacggaccgg cgctgtcgct gccgttatat ccgtgcgata acgtcgattt
     5101 ccaggtcacc acctactaca gcaacatttg cccaaatggt gcttatcagg gttatggcgc
     5161 accgaaaggt aacttcgcta tcaccatggc attagcggaa ctggctgaac agttacagat
     5221 cgaccaactg gaaattatcg aacgtaaccg ggtacacgaa gggcaagagc tgaaaattct
     5281 cggtgcaatc ggtgaaggta aagcgccgac ctccgttcct tccgccgcca gctgtgcact
     5341 ggaagagatc ctgcgtcagg gtcgcgagat gatccaatgg tcgtcaccga aaccgcaaaa
     5401 tggtgactgg cacatcggtc gcggcgtcgc cattatcatg cagaaatcgg gtatcccgga
     5461 tatcgatcag gctaactgca tgatcaaact ggaatcggac ggcaccttta tcgttcattc
     5521 tggcggtgcg gatattggta ctggtctgga tactgtagtg acgaaactgg cagcagaagt
     5581 gctgcactgc ccaccgcagg acgtgcatgt tatctccggt gataccgatc atgcgttgtt
     5641 tgataaaggc gcatatgcct cgtccggtac ttgcttctcg ggtaacgcgg cgcgtttggc
     5701 agcggaaaat ctacgggaga aaattctgtt ccacggcgcg caaatgttgg gtgaggcagt
     5761 ggcagatgtt caactggcaa cgccgggcgt cgtgcgcggc aagaaaggcg aagttagttt
     5821 cggggatatt gcccataaag gcgaaaccgg catcggcttt ggttcactgg tgggaactgg
     5881 cagttatatc acgcctgatt tcgccttccc gtatggcgca aacttcgctg aagttgccgt
     5941 caacacgcgt acgggtgaaa tccgcctgga taaattctac gccttgctgg actgcggtac
     6001 accggtcaac ccggaattag cattgggaca aatctacggt gctaccctgc gagctatcgg
     6061 ccacagtatg agcgaagaga tcatttatga cgccgaaggt cacccgttaa cgcgtgattt
     6121 acgcagttac ggcgcaccga aaattggtga cattccgcgt gatttccgcg ctgtgctggt
     6181 gccgagcgac gataaagtcg gcccgttcgg ggcgaaatcg atctcggaaa tcggtgtaaa
     6241 tggcgcagct ccggcgattg ctaccgcaat tcacgataca tgcggcatct gggcagaaga
     6301 aagtcaaagt gggcatggga acggatatcg gtgccggaac aactttcaac atgctgcaaa
     6361 cgctgaacga agcctacaaa gtgttgcaat tacaaggcta tcgcctctcg gcatatgaag
     6421 cgttttacct ggccacgctc ggcggagcga aatctctggg ccttgacgat ttgattggca
     6481 actttttacc tggcaaagag gctgatttcg tggtgatgga acccaccgcc actccgctac
     6541 agcagctgcg ctatgacaac tctgtttctt tagtcgacaa attgttcgtg atgatgacgt
     6601 tgggcgatga ccgttcgatc taccgcacct acgttgatgg tcgtctggtg tacgaacgca
     6661 actaataata aaactttaac atcctcgtga ggacatcatt atgtctggag acatcctaca
     6721 aacaccggac gcaccaaagc cacagggcgc gctggataat tattttaaaa ttaccgctcg
     6781 tggcagtacc gttcgtcagg aagtactggc tggcttaacg acctttctgg ccatggttta
     6841 ttccgttatc gtcgttccgg gaatgctggg caaagcagtt tttcctcccg cagctgtgtt
     6901 tgttgccacc tgtctggtcg cgggcttcgg ctcgttgctg atggggttgt aggccaattt
     6961 gccaatggcg attggttgtg cgatttcctt gacggcattt accgcattca gtctggtact
     7021 cgggcaacaa attagcgttc ctgtcgcact ggtcgcattt tacgcgggtg gttgggcaat
     7081 ctaatagatt ctccgcgcct ttcagcgcgg agatttcttt cacagaggat tctcaccgct
     7141 ggcaacaata cgctgctggc ggactcgcct gagttctctt tcagtaagca actgcagcgc
     7201 ctgtgtcggg caagattcaa cacaggctgg cccctcttcc cgctgttcac agagatcgca
     7261 tttcacaatt tgttgtcggg tatcccccgg caacgactga atggtgatca ttccaaacgg
     7321 gcacgcactg acgcaactct gacagccaat acaacgggca gaattggtct gcaccacctg
     7381 ctcgcccatc gtcagcgccc ccacggggca agcgccaaca caaggggcgt tttcacactg
     7441 atggcacatc accggcgcac tgatgctgtc cagtcgctgt accttcagcc ggggtaaaaa
     7501 gacatcggca ttcaactcct gttctgaagg gtgagcgacc acacaggcta cttcacaagt
     7561 acggcagcca atacagtcag ccggattaac gataattaac gatttcatca cgatgccttc
     7621 gtatcaaaca gagttaacat atcgcgcgcc gcctgccttc ctgcggccat tgcagtgaca
     7681 accagatccg cgccatgaac tgcatcacca ccagcaaaga cttttttcag atgcgtctgg
     7741 gtaggtaaat acccgacgtc accggtttga atcaggcccc atttatcgag tttaattccg
     7801 ctgccctgca accacggcat ggcatgcgcc tggaaaccaa aggccataat cagaacatcg
     7861 gcgggcaatt caaactctga acccgctacc ggacgaggac gacggcgacc atccggcccc
     7921 ggctcaccca tggcggtacg aatcaggccc accgcagtta aacgcccatc ttcgtcacaa
     7981 gcgatatatt gcggttgaac attgaactga aactcgacac cttcctcgcg cgcattaacc
     8041 acctctttgc gcgagcccgg catactgact tcatcacgac gatacgcgca ggtcacgctg
     8101 gcagcattga ggcgaatgga agtccgcaaa caatccattg tcgtatcgcc accgcccaat
     8161 accacgactc gcttaccttc cacgtccgtc agcggatact cttcagactc cggcaatccc
     8221 atgagctggc gggtatgggc agtcaggaac ggtagagcct gaatgacacc gggcgcatct
     8281 tcatgcggca gatctgctcg catcatcccg taagtcccca cgccgatgaa aactgcatca
     8341 tattcagaag ttaaatcgct aaaggtaata tcgcggccaa tttcacagtt aagatgaaag
     8401 tcgattccca ttgcggtgaa tatctctcgc cgctggctta ataccgtttt atcgagtttg
     8461 aaaggaggaa tgccaaaagt cagcataccg ccaatttctg gatggcgatc aaagacatca
     8521 acctgaactc ccgcgcgcgc cagaatatca gcacacccta accctgctgg cccagcgcca
     8581 atcaccgcca ctttttcgat acggggaaca actttgctga catcgggacg ccagcccatc
     8641 gccagcgcgg tatcggtgat gtagcgttcc agattaccga tagagactgc gccagagtga
     8701 tctttcaaag tacatgcgcc ttcacaaaga cggtcctgtg ggcataccct gccgcagatt
     8761 tctggtaagg aactggtctg gtggcaaagt tctgccgctt caataatctt tccttcctgt
     8821 accagacgga tgtaatccgg aatagcgtta tgcagcgggc aatgccagtt gcagttagct
     8881 ttttcggcac aataaacaca gcggtcactc tcataagtcg cttgcagtgg atccagcccg
     8941 caatagattt cgccaaagtg ggttttccgt tcactcgctg aaattttatc tgcgccttta
     9001 cgcgagttaa cggggagcaa cgctgcactg cgagatggct gagcgtctga tgacgctttt
     9061 cctgctgccg ttttgcgctg gcgagccacc tttatccact gtaacccttt atcgtccatc
     9121 agtcgtaacg cctgtgttgg gcagacttca atacaggctt gcgtgccgga actgcgctgg
     9181 ttacaaaggt cgcatttctg cgcaatcgta tcgaccatct caacgacgcc aaaggggcaa
     9241 gcgattgcgc atcttttaca gccaatacat ttttgctcgt ccagttgtac gctatcggac
     9301 tggaaagtca gagcattaac cggacaagcc gtaacacaag gggcattgtt gcagtgatgg
     9361 caggccaccg gattcgcagc ctggcctttc cctacaacgt ggatacgcgg tcgaaagtca
     9421 ctgtgactca gcggccagtt ttcttgattg tgtgccaccg cacaggcaat ttcacaagca
     9481 tgacagccta tacattccgc agcttcagca gcgataaact tattcatttg catccctttc
     9541 at
//
LOCUS       DAJVRF010000142         9500 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJVRF010000142 DAJVRF010000000
VERSION     DAJVRF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9500
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.142"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(149..1231)
                     /locus_tag="OQN13_003307"
     CDS             complement(149..1231)
                     /locus_tag="OQN13_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HCS1616585.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3024)
                     /locus_tag="OQN13_003308"
     CDS             complement(1228..3024)
                     /locus_tag="OQN13_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCS1616586.1"
                     /translation="MNVQHFDFFTDNEFSNLERYQTCKAMAKGYAGNYKTGQSRLMHA
                     RSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAERTGELIAQFPAE
                     DAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDFSHASVIDPACGG
                     GAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLVLLESVLMPLCVK
                     VKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKYSRSLFGHANLYG
                     LFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYALDFVTDRDGVFD
                     DVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKGGATWLLPRVKDD
                     ACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPLVWAESITSAGFR
                     FSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAILPQGFIDETGGV
                     VVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVSAYELNSIPLPSL
                     EQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="OQN13_003309"
     CDS             complement(3275..3631)
                     /locus_tag="OQN13_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616587.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3689..4111)
                     /locus_tag="OQN13_003310"
     CDS             complement(3689..4111)
                     /locus_tag="OQN13_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCS1616588.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="OQN13_003311"
     CDS             complement(4127..4852)
                     /locus_tag="OQN13_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCS1616589.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4874..5620)
                     /locus_tag="OQN13_003312"
     CDS             complement(4874..5620)
                     /locus_tag="OQN13_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS1616590.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6691)
                     /locus_tag="OQN13_003313"
     CDS             complement(5627..6691)
                     /locus_tag="OQN13_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HCS1616591.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGGPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6763..7188)
                     /locus_tag="OQN13_003314"
     CDS             complement(6763..7188)
                     /locus_tag="OQN13_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1616592.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7185..7448)
                     /locus_tag="OQN13_003315"
     CDS             complement(7185..7448)
                     /locus_tag="OQN13_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1616593.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7560..8060
                     /locus_tag="OQN13_003316"
     CDS             7560..8060
                     /locus_tag="OQN13_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1616594.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            8080..8274
                     /locus_tag="OQN13_003317"
     CDS             8080..8274
                     /locus_tag="OQN13_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCS1616595.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8274..8564
                     /locus_tag="OQN13_003318"
     CDS             8274..8564
                     /locus_tag="OQN13_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCS1616596.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8844..8996
                     /locus_tag="OQN13_003319"
     CDS             8844..8996
                     /locus_tag="OQN13_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCS1616597.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            8996..9373
                     /locus_tag="OQN13_003320"
     CDS             8996..9373
                     /locus_tag="OQN13_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616598.1"
                     /translation="MRLTMEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARK
                     IKTAFVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRR
                     GLDGSYDHDVLQVLRTMVTSLGI"
BASE COUNT         2443 a         2493 c         2016 g         2548 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatc tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctggcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ccggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc caccacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggcc ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctgtcgtg actcgtcgtg acatgtgcgt gacatttcgt gacgcgccgt
     6301 gacaatcgcc attttgttcc cgctttcttt ccctctctcg ctgcgccctc ttgcgctctg
     6361 caggagattt tgcggtttgc gaaatattgc cgttgtcctc tttaagcacc tggcgttttt
     6421 cccatccagt gattaaatca ccatcaagta cccgcccctg catcgtctgc aaaattgaat
     6481 caattacctc ttctgtcacg tcgagcgcac ttgccaaatc ttctgtcgtg acatcaatgt
     6541 gacctcgcgt gacatttcgt gacgcgctca ccaggaggtg gatatacact gccatcactg
     6601 ttgcaattgg ctgccctgac accctggcaa ttgttcgcca cttagggtca tttggcatgt
     6661 catgccataa tctgagccag gcgttagcca tactcacctc ttctgatacc gaatcttttt
     6721 actcacgagt tgccgggagc gattcgatat ggctattgtc agtcaatgta ctgccacagc
     6781 atttcctgcc gggccaccac ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa
     6841 atcatcagcg tctctcacca gtcgttcatg cgtctccacc agttcccgaa aataagctga
     6901 actgtggctg cgcattcgag ccaccagcgg aggtggcatt gccctttcga tcgcaggtaa
     6961 caacgcctga attttttcaa cagcgtcagg ggtgtcttta tccagccaac ggaaaatctt
     7021 ttgggtgttg agataaagcg tgttgtgctt gctgtcgtta tgcagaaccg gaaacgtcat
     7081 tcccagttcg aaataaaccc tggttatttc agctgccgga actttttcac cgtccggatg
     7141 cgcccaggct ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcaaccccc
     7201 gccgatggtt gtgtgttagc ctgaaactca gcaggcaagc cgtcagttgg gttggggtag
     7261 gttttactgt caatctcatg tggggtcacc acccatcccg ttgcttcaca ccaacgtaat
     7321 attttttctc ctgtaagttt tgcacgcccc gttataacgt ggctgaccat cccttgggat
     7381 gcgccaacaa tctcagcaaa tgaactctgg gttatatgaa aatgatgcaa atattctcca
     7441 agattcataa tccgccctat gtgatgtgtt accaatgcat aattaatagc actgttattt
     7501 ttaaaagtaa atagtgatac tatttcaaag tgattagcaa tcttattaga attggatgta
     7561 tgaaaagaaa atcattgtca gacaccgacc tcaacgccgc cagaagactg aaagaaatct
     7621 ggatggcaaa gaaatctcag ttgggattga ctcaggaacg tgcggcggaa attatggggt
     7681 tttcaacaca aggggcggta agtcactacc taaatggtca aaccccacta aaccttgagg
     7741 ctgttctaaa gtttgctggc ttactgaaag ttccccctga gtcaatcagg ccagacatgg
     7801 tagatttgct gcaaattgca aggatgtatt gtcgagaacc acagcaagat aacatagtcg
     7861 cattaccagc agacacagaa caaacagagg atgaactacc gtttgccgta gagccgatgg
     7921 aacgagattt aatccatacg ttcagagctt ttccgaaaga agatcaagaa cacatgcttc
     7981 aggagatgaa agataaaaaa gaatcaatgg atcgtacggt tgcgcggtgg ttagctgcac
     8041 aaaaaggccg tcgcgcctaa atcagaggag atatgtaaaa tgagtacagt cctttcccca
     8101 atggtttcag aatttgagac agttgagcaa gaaaatagtt acaacgaatg gctgcgggcc
     8161 aaagtagcag caagcctcgc ggatccccgc ccggcgattc cgcatgatga ggtaatggcg
     8221 gaaatggaac aaattattgc ccaaattgct gctgcaactc acagaggtga ataatgttac
     8281 ccgttttatg gctaccatct gctcgtgatg atttgcgcca gatcgtagcc tacatagcta
     8341 aagaaaatcc cgcggcagca cgcagaatga aaattcgaat tgaaacatcc gttttgccgc
     8401 tcacagagca tccttacctg tatcctccaa gcgagcgagt tcttggtctt cgtgaaattg
     8461 taactcatcc taactacatc atcctttacc gagtaacagc atcaagcata gaagtagtaa
     8521 atattgtgca ttctcgaaga cagtatccag gcaaaaacag ttaattattc ccgccgacag
     8581 aaccacctta gggtggtttt ttcttgcccc aacaaaaaac aatagcgatg ctatttacat
     8641 caaaaaaaat aatgatgcta ttgtttcagg cgtcaaccca ccccgcccca cagaatgcag
     8701 ggcaatactt cgagttacca gacagtggtc aggggttaag tagccagccc gaggcgtaag
     8761 aacatgacgg cagggttcaa tctttaacta tgcagcaggt tttttgttcc gccaccccgg
     8821 cgttaagggg aaatgaggtc agcatggata ctatcgatct tggcaacaac gaatctctgg
     8881 tgtgcggcgt gttccctaac caggatggaa cgtttaccgc catgacgtat accaaaagca
     8941 aaacgtttaa aaccgaatct ggcgcgcgtt gctggttagc cagaaacact gactgatgag
     9001 gctgacgatg gaatttaaag atttaccgtc tgatgtacag aaaacagcag ctcatacatt
     9061 gcattctgtg ctgcgagaaa tcgggaaaga tattgcaagc gagccagcaa aagatctggc
     9121 ccggaaaatc aagaccgctt tcgttgagct ttataatgtt ggcactgact ctgaaactgt
     9181 cgagaccaag accgtaagtt caccaatatt ctcacttgag ccaagcgtat tatcaggtga
     9241 aatatgcacc gagatttcca gcgaactgct tccggtaata cgagaagcaa ttcgccgtcg
     9301 cggtttggat ggaagttatg atcatgacgt tctgcaagtt ctcaggacaa tggtgacttc
     9361 actggggatt tgatccctgc gtcctctaag cttttgatat agcggtcctc caagaagcgg
     9421 taaattttgt cgaaatcatc ttttccctct ggtttattca gaggattaca ggacccgtaa
     9481 gtagcggcat acatttcaac
//
LOCUS       DAJVRF010000143         9436 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJVRF010000143 DAJVRF010000000
VERSION     DAJVRF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.143"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(111..1106)
                     /gene="gapA"
                     /locus_tag="OQN13_003321"
     CDS             complement(111..1106)
                     /gene="gapA"
                     /locus_tag="OQN13_003321"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCS1616599.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1448..1861
                     /gene="msrB"
                     /locus_tag="OQN13_003322"
     CDS             1448..1861
                     /gene="msrB"
                     /locus_tag="OQN13_003322"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCS1616600.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1903..2175
                     /locus_tag="OQN13_003323"
     CDS             1903..2175
                     /locus_tag="OQN13_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCS1616601.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2336..2977)
                     /gene="pncA"
                     /locus_tag="OQN13_003324"
     CDS             complement(2336..2977)
                     /gene="pncA"
                     /locus_tag="OQN13_003324"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCS1616602.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2988..4004)
                     /gene="ansA"
                     /locus_tag="OQN13_003325"
     CDS             complement(2988..4004)
                     /gene="ansA"
                     /locus_tag="OQN13_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCS1616603.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4171..6027)
                     /gene="sppA"
                     /locus_tag="OQN13_003326"
     CDS             complement(4171..6027)
                     /gene="sppA"
                     /locus_tag="OQN13_003326"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCS1616604.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6188..6739
                     /locus_tag="OQN13_003327"
     CDS             6188..6739
                     /locus_tag="OQN13_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCS1616605.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6856..7899
                     /gene="selD"
                     /locus_tag="OQN13_003328"
     CDS             6856..7899
                     /gene="selD"
                     /locus_tag="OQN13_003328"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCS1616606.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDATEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7904..>9436
                     /gene="topB"
                     /locus_tag="OQN13_003329"
     CDS             7904..>9436
                     /gene="topB"
                     /locus_tag="OQN13_003329"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCS1616607.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIIPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKD"
BASE COUNT         2335 a         2362 c         2495 g         2244 t
ORIGIN      
        1 caagtgagcg tttccggatt cttacacagc cactggatcg gtcaactgat ccttaactga
       61 tcggcattac gaccgaagtc gctcttttta gatcacagtt tcatctcaac ttatttggag
      121 atgtgagcga tcaggtccag aactttgttg gagtaaccgg tttcgttgtc gtaccaggat
      181 accagtttca cgaagttgtc gttcagagcg ataccagctt tagcatcgaa cacggaagtg
      241 caaacttcgc cgttgaaatc ggtagatact acgtcatctt cggtgtagcc cagaacgcct
      301 ttcatttcgc cttcagcagc ggctttaacg gcagctttga tctgctcgta agttgcagct
      361 ttttccagac gaacggtcag gtcaactacg gatacgttcg gggtcggaac gcggaacgcc
      421 ataccagtca gtttgccatt cagttctggc agtactttac ctacagcttt agcagcaccg
      481 gtagaggacg ggatgatgtt ctgggaagcg ccgcggccgc cgcgccagtc tttgtgagac
      541 gggccatcaa cggttttctg agtagcggta gtagcgtgaa cggtggtcat cagaccttcg
      601 atgatgccga agttatcgtt gataacttta gccagcggag ccaggcagtt ggtggtgcag
      661 gaagcgttgg aaacgatgtc ctggccagca tatttgtcga agttagcgcc tttaacgaac
      721 atcggagtgt tgtctttaga cggaccagtc ataaccactt tcttcgcacc agcggtgatg
      781 tgtttacgag cagtttcgtc agtcaggaac agaccagttg cttcagcgac aacgtcaaca
      841 ccaacttcgt cccatttcag gttagccgga tcacgttcag cggtaacacg gattttttta
      901 ccgttaacga tcagatgacc gtctttcact tcaacggtac cgtcgaaacg gccgtgagtg
      961 gagtcatatt tcagcatgta tgccatgtaa tcagcgtcta acaggtcgtt gattgcaacg
     1021 atctcgatgt cagaacgttt ctgagcagca cggaaaacaa tgcgaccgat acggccaaaa
     1081 ccgttgatac ctactttgat agtcatatat tccaccagct atttgttagt gaataaaagg
     1141 ttgcctgtaa aattacaaaa accttacgca gcgtcaagcg gaatcgtgtc aatcattgcg
     1201 acaaatcaat cctgtgccta agcattacgc gactgactcg cctcactctt cctttgggct
     1261 tgaaaccaca tggggtcggc gccccgaatt ttaaagggca attacgataa aaatgtgatt
     1321 ttcatcacga tttaggtgca gccatttcgt cgagatcaag tttaggacaa aattttgcca
     1381 gttggtgtta atgttttgtt agaatcagtc acgtaatgtg agcacgatta aagtgagatg
     1441 tgagcaaatg gctaataaac cttcggcaga agaactgaaa aaaaatttgt ccgagatgca
     1501 gttttacgtg acgcagaatc atgggacaga gccgccattt acgggtcgtt tactgcataa
     1561 caagcgtgac ggcgtatatc actgtttgat ctgcgatgcc ccgctgtttc attcccaaac
     1621 caagtatgat tccggctgtg gttggcccag tttctacgaa ccggtgagtg aagaatccat
     1681 tcgttatatc aaagacttgt cacatggaat gcagcgcata gaaattcgtt gcggtaactg
     1741 tgatgcccat ctggggcatg tcttccctga cgggccgcag ccaacgggcg aacgttattg
     1801 tgttaactct gcttctttac gcttcactga tggcgaaaac ggcgaagaaa tcaacggttg
     1861 aaaaaacgat tcagcaactt attccacagg agcggaaaaa ttatgaatct tgatgacatt
     1921 atcaacagca tgacgcctga ggtataccag cgtttgtcga ccgccgttga actggggaaa
     1981 tggcccgatg gcgttgcgtt gaccgaggaa caaaaagcga actgcctgca actggtgatg
     2041 ctgtggcaag ctcgccataa taccgaagca cagcatatga caattgacac aaatggtcag
     2101 atggtcatga agagcaaaca gcagttaaaa gaagattttg gtatctcagc aaagcctatt
     2161 gctatgttta agtgaatgag ttatctagta tttaagcctg ttattaaggg agggcaaata
     2221 tacttgttag gacgtcgtgt tttagctgca agtaggttaa atgcaacact gacaagtatt
     2281 atctggccta caatgtcatt gaggaaatgt aggccagaga tgaattcaac taatattacc
     2341 cctgtgtctc ttcccagtct gccagcgtat atagcgttgc cccagctgct gacatctcca
     2401 taaacgcgtg cgcactgtcc tggggctgga tattcacgcc acgacaacca tcggtaatca
     2461 catttacctt ataacctaac tgtaacgcgt ccagcacggt aaacttcacg caatagtcag
     2521 tagccaggcc cataacgatc aattcatcga tttcatgatc gcgtaaccag tcatcgagcg
     2581 aggttttctg ccgacggccg ttatcaaaaa aggcactgta actgtcaact aaaggatttt
     2641 cgcctttatg gaacaccgct gcgatcgctt tttggttcag taacggatgt aattgtgcgc
     2701 cttcactgtt ctgcacacag tgatctggcc agaaggtttg tggcaaaccg tcgagttggc
     2761 ctggcgtata aggctctaca ccgtgctgac tggcaaaact gccgtgattc gccgggtgcc
     2821 agtcctgact ggcgataacc gcttcaccgc gcgactggca ccagtcaatc aggcggttag
     2881 cgacatccac cgtactgtca ccttccggca cggcgagcgc gccaccagca cagaaatcat
     2941 tttgtaaatc gaccagtaac agggcgcgag ggggcattac agtctcctta atcatccggc
     3001 gtcagttcgc cgcgcaggtt ttggctcatg gccttgcgaa tggtttcagt atccagttcc
     3061 tggctcagca ggtaatgcag tttggttagc gtggcttcta cagtcatatc tgcaccgcca
     3121 ataacgccgg catgggcgag ggcgttaccg gtggcataac cacccatatt cactttaccg
     3181 gacatacatt gtgtcaggtt gaccaccaca ataccgcgat cgctggcttc ttgtaattcc
     3241 tgcaggaagg ctttgttttg tggcgcatta cccacgccat aggagcgcag aatcaatgct
     3301 ttcaccggtt ggcgcagaaa attgcgcacg acgtcagcag aaatccctgg ataaatcgtc
     3361 actacgccaa ttggttgtgg cgtgattgga tgaacgatta attccccttc accgtgcggg
     3421 gcgggtggcg tattcaaacg acgaatatgg ataccagctt ccagtaacgg aggaaggttt
     3481 ggagaggcaa acgcatcaaa accatcggca tgggctttgg tagtgcggtt gccgcgatac
     3541 aatcggttat tgaaaaagag cgttacttcg ttgatcggat aattcgccgc aacgtacaac
     3601 gcattcagca gattaatttg tccgtcagag cgtaactcag ccagcgggat ttgtgaccct
     3661 gtcacaatga ccggtttacc gagattctcg agcatgaacg acagcgcaga ggcagtatac
     3721 gccatcgtgt cggtgccgtg caggatgaca aaaccatcat agtcgtcata gtgcgcttta
     3781 atatcttcag caatatgctg ccagtcttct ggcgtcatat cagaagaatc catcagcggc
     3841 gtatattcat gaatggtgaa atctggcatc tccgggcgat ggaattccgg catcagcgcc
     3901 agttggcgtt gtagatgacc tgacaccgga atataaccct gctcggaacg ctgcatcccg
     3961 atggtcccgc ccgtgtaggc gacgtaaatt gatttctttt gcatgatatt gatatgaatg
     4021 atatcgaaag agcaaaagta tatacgtgag gcaggaggag cgcacctgat actatgcgga
     4081 taaggaaata tggggcgaga cagcgggaag aggattcgtc gacgtaaaaa ataatgtggc
     4141 cgacgatctg tcggccactc agtacaagac ttaacgcacg ttggcgcagg tcaggcaaaa
     4201 cgcataacgg ttttgtgggt cgttaaacgc ggccagcttg tcgctttcac ttttaacggt
     4261 agaggctacc gaggcaagcg gtgcaggtaa catggcctgg aacgcatctg gcaacattgc
     4321 ccggacagaa ccagacatgt tgtccatcac tttgtcgaag aaggtcggtt catcaacgta
     4381 gtattccaga tgccactgtt tcacttttgc cagctctgct gctttggcga ccgcatcatc
     4441 gaaatccccg agactatcga ccagcccgtt agcttttgca tcctgaccgg tccagacgtg
     4501 gccctgggcg attttatcaa tctgctccgg cgtcgaatga cgcgcatcag caaccagcgt
     4561 gataaagcgt ttatagccat tctcaatgct taattgcatc atctgctgcg cttccggcgg
     4621 cagtgccctg gtgatagaaa catccgccag cggtgaagtt gagacaccat cagtatgaac
     4681 accaatcgaa tccagactat tttctacggt ggtgatcacg ccgaagatac cgatagaacc
     4741 ggtcagggtg ctggggttag ccacaatgta attagctggc gtggaaatcc agtaaccacc
     4801 agatgccgcc atgccgccca tcgatacaac cacaggctta cccgctgccc gggctgctgc
     4861 cagttcagcg cgaatcactt cagacgcggt aacgctgccg cctgggctat taacacgcag
     4921 gacaatcgct ttcactttcg ggtcaaggcg agcgtcgcgg atttgtgccg cagtggtatc
     4981 accgccaaca ttcccctgag tttcctcgcc atccataatt gcgccattag cgaagacgac
     5041 accgatgctg tcaccggtat ctgccggcgt tttcaatgcg taatcgtaat aactgatggc
     5101 gcgataattt ttatcagtct tactccagcc gaactctttg gttagagctt tttcgatttc
     5161 ggcactcgat gccagtgcat cgaccagctt gttttccagt gcatatttcg cggtatcgcc
     5221 accggttttg gttaaaccct caagcaaccc ttgcgcgcca gggaatacct gctgagcagg
     5281 gatctgccgg ttagcggcaa cagtattcag atagttttgc cacagctcac caatccagcg
     5341 gctgtcagct tcgcgggctg ccggtgacat gtcatcacga ataaacggtt caacggcaga
     5401 tttatacgta cccacgcgga acacatgggt ggaaactttc agcttatcca gcaacgattt
     5461 gtagtacaga ccgttggtag caaaaccgtg caaatccacc acgccttgcg gtgacagcca
     5521 aattttattg gcgaaactgg cgagataata ttgcccctgg ctgtagttct cgccaacggc
     5581 ataaaccggt ttcccgctgt cacgaaactc tttcagggct ttgccgatgt actgcataga
     5641 cggctggtcg ccgcctgcga agtttttcag atccatcacg ataccggtga tattgcggtc
     5701 gtccttcgcc tggcgaatgg tgttgacgat atcaaacagt gagttttcct gcagacgatc
     5761 ggaactggca ccaagcagct ggcggcttaa tttactaaac cgctgagaac tgtcgggttt
     5821 atcgacgatc acaccagaaa tgtccagcag cagtgcgcca cgactggccg tttctttcga
     5881 atcaccacca ctgacctgca tccagatccc cacaccaacc agtacgagga aaataaagaa
     5941 caggttaagt accatttcac ggacgaaatt cagcagacgc cacgtccatt taaaaaatcc
     6001 ggcaataaat cgccaaaggg ttcgcatgta ttctcccaac ttaaggtcac acctgtcaca
     6061 ggcgcaatag atgtggttat cgtaatgaca caaccggcac ttgtcagcag gaattgcgga
     6121 ctgtgctgta acaaaatcca ctctcgtgtt aattttgtga ataaatatca cgacaggagt
     6181 taatcaaatg gatgcactcg aactattgat caatcgccgt agcgcctccc gcctggctga
     6241 acccgcgcca acgggtgaac aactgcaaaa catcctgcgt gcgggtatgc gtgcgccgga
     6301 ccataagtcc atgcaaccgt ggcatttttt tgtgattgaa ggggaagggc gcgagcgttt
     6361 cagcgccgta ctggaacagg gggcgattgc tgccggtagt gatgacaaag ctatcgacaa
     6421 agcccgtaat gcgccgttcc gcgcaccgct catcatcacg gtggtggcga aatgcgaaga
     6481 aaatcataaa gtcccgcgat gggaacagga aatgtctgcc ggatgcgcgg tcatggcgat
     6541 gcaaatggca gccgttgccc agggatttgg cggcatctgg cgcagtggcg cattaactga
     6601 aagtccggta gtgcgtgaag cattcggttg ccgtgagcag gataaaattg tcggttttct
     6661 ctacctcggc acgccgcagt tgaaagcatc tacgtcgatt aacgtcccgg acccgacgcc
     6721 gtttgtaact tatttctgat aattgtcgcg aaactgtctg gattgtgagc aaaggcgcag
     6781 gaattcagac actctcactt atcacttcac ggaatgaggg ttaccatacc cggattgcat
     6841 tgacaggaga tgtccatgag cgagaactcg attcgtttga cccaatacag ccacggagct
     6901 ggttgcggct gtaaaatttc cccaaaagtg ttggaaacca tcctgcacag tgagcaggcg
     6961 aagtttgttg atccgaattt gcttgtgggt aatgagaccc gcgacgatgc ggcggtgtac
     7021 gatctgggca atggcaccag cgttatcagt accaccgact tctttatgcc gatcgttgat
     7081 aatcctttcg attttggccg cattgcggcg actaacgcca tcagcgatat cttcgcgatg
     7141 gggggcaaac cgattatggc gattgcgatc ctcggctggc cgattaacaa actttccccg
     7201 gaaattgccc gcgaagtaac cgaaggtgga cgctatgcat gccgtcaggc gggtattgcg
     7261 ctggctggcg gtcactccat cgatgcgacg gagccgattt ttggtctggc ggtaacgggg
     7321 atcgtaccga ccgagcgggt gaagaaaaac agtaccgcac aagccggatg caaactgttc
     7381 ctgacgaaac cgctggggat cggcgttctt accacggctg agaaaaaatc actgctgaaa
     7441 ccagaacatc agggactagc gacggaagtg atgtgccgga tgaacatcgc aggcgcgtcc
     7501 tttgccaaca tcgaaggcgt aaaagcgatg accgacgtta cgggttttgg tctgctgggc
     7561 catttgagcg aaatgtgtca gggcgcaggt gtgcaggcgc gcgttgacta tgacgcgatc
     7621 ccaaaattgc cgggggttga agagtacatt aagttgggcg cagtgcctgg cggcaccgaa
     7681 cgtaactttg ccagctacgg tcatctgatg ggtgaaatgc cgcgtgaagt gcgcgatttg
     7741 ctgtgcgatc cgcaaacttc tggcggtttg ctgctggcgg tcatgccgga agcagaaaat
     7801 gaggtcaaag ctacagccgc cgagtttggc attgaactga cggcaattgg cgaactggtg
     7861 ccagcgcgcg gcggtcgtgc catggttgag attcgttaat tcaatgcggt tgtttattgc
     7921 cgaaaaaccg agtctggcgc gcgccattgc tgatgtcctg cccaaaccgc accggaaagg
     7981 cgatggcttt atcgagtgcg gtaatggtca ggtggtgacc tggtgtatcg gtcacctgct
     8041 tgagcaggcg cagccagacg cctacgacag ccgctatgcg cgctggaatc ttgcggattt
     8101 gccgattatc ccggaaaagt ggcaattaca gccccgaccc tccgtgacca aacaacttaa
     8161 cgtcatcaaa cgcttcctgc atgaagccag cgaaatcgtt cacgccgggg acccggatcg
     8221 tgaagggcaa ctgctggtgg atgaagtgct ggactatctg caactggcac cggaaaagcg
     8281 ccagcaggtg cagcgttgct tgataaacga cctgaacccg caggcggttg agcgggcgct
     8341 cgaccgtctt cggtccaaca gtgagtttgt gccattgtgc gtttctgcgc tggcgcgagc
     8401 gcgtgccgac tggctgtacg gcatcaatat gacccgtgcg tataccattc tcggtcgcaa
     8461 tgccggttat cagggcgtac tttccgtggg acgcgtgcag acacccgtgc tcgggctggt
     8521 ggtgcgccgc gatgaagaga ttgaaaactt cgtggcgaaa gacttctttg aagtcaaagc
     8581 acatatcgtg acacctgccg atgagcggtt taccgctatc tggcaaccga gcgaagcgtg
     8641 tgaaccgtac caggatgaag aagggcgctt gttacatcgt ccactggcgg agcatgtggt
     8701 taaccgcatt agtggtcaac cagcaattgt caccagctat aacgataaac gggaatcaga
     8761 atccgcgccg ctgccttttt cgctttcggc gttgcagatt gaagcggcaa aacgctttgg
     8821 tctgagcgcg cagaacgtgc ttgatatctg ccagaagctg tacgaaacgc acaagctaat
     8881 cacttatccg cgttccgatt gtcgctattt gccagaagaa cattttgccg gacgccacgc
     8941 ggtgatgaat gccatcagcg ttcatgcacc agatctgttg ctgcagccag tggtagatcc
     9001 agatatacgc aaccgctgtt gggatgacaa aaaggtcgat gcacaccacg ccatcatccc
     9061 gaccgcacgg agttcagcca tcaacctgac ggagaacgaa gcgaaggtct ataacctgat
     9121 tgcccgtcag tacttgatgc agttctgccc ggatgtggtg ttccgcaagt gtgttatcga
     9181 actggacatt gccaaaggca aatttgtcgc taaagcgcgt tttcttgctg aagcaggctg
     9241 gcgcacgctg ttaggcagca aagagcgcga tgaagaaaac gacggtacgc cattgcctgt
     9301 ggtggcgaaa ggcgatgagt tgctgtgtga aaaaggtgaa gtggtagagc ggcaaaccca
     9361 gccgccgcgc cattttaccg atgcaacact gctttcggcg atgaccggga tcgcgcgctt
     9421 tgtgcaggac aaagat
//
LOCUS       DAJVRF010000144         9433 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJVRF010000144 DAJVRF010000000
VERSION     DAJVRF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9433
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.144"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            206..1072
                     /gene="folD"
                     /locus_tag="OQN13_003330"
     CDS             206..1072
                     /gene="folD"
                     /locus_tag="OQN13_003330"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCS1616608.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1074..1286
                     /gene="ybcJ"
                     /locus_tag="OQN13_003331"
     CDS             1074..1286
                     /gene="ybcJ"
                     /locus_tag="OQN13_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCS1616609.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1394..1915
                     /locus_tag="OQN13_003332"
                     /pseudo
     CDS             1394..1915
                     /locus_tag="OQN13_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
     gene            complement(1951..3336)
                     /gene="cysS"
                     /locus_tag="OQN13_003333"
     CDS             complement(1951..3336)
                     /gene="cysS"
                     /locus_tag="OQN13_003333"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCS1616610.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3510..4004
                     /gene="ppiB"
                     /locus_tag="OQN13_003334"
     CDS             3510..4004
                     /gene="ppiB"
                     /locus_tag="OQN13_003334"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCS1616611.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4007..4729
                     /gene="lpxH"
                     /locus_tag="OQN13_003335"
     CDS             4007..4729
                     /gene="lpxH"
                     /locus_tag="OQN13_003335"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCS1616612.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4847..5356
                     /gene="purE"
                     /locus_tag="OQN13_003336"
     CDS             4847..5356
                     /gene="purE"
                     /locus_tag="OQN13_003336"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCS1616613.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVLHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5353..6420
                     /gene="purK"
                     /locus_tag="OQN13_003337"
     CDS             5353..6420
                     /gene="purK"
                     /locus_tag="OQN13_003337"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCS1616614.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6557..7450)
                     /locus_tag="OQN13_003338"
     CDS             complement(6557..7450)
                     /locus_tag="OQN13_003338"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCS1616615.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7447..8100)
                     /locus_tag="OQN13_003339"
     CDS             complement(7447..8100)
                     /locus_tag="OQN13_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCS1616616.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8111..9370)
                     /locus_tag="OQN13_003340"
     CDS             complement(8111..9370)
                     /locus_tag="OQN13_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCS1616617.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2102 a         2673 c         2498 g         2160 t
ORIGIN      
        1 ttagacaagg aaataagtca gttccagatt tacaacgcca tcatggacga aaaatgaagc
       61 tttcaatctc agcgacggtg cgccccacct tcgcaagagg tcgcttcacg cgataaatct
      121 gaaacgaaac ctgacagcgc gccccgcttc tgacaaaata ggcgcatccc cttcgaccta
      181 cgtaacagat ggaatcctct ctctgatggc agcaaagatt attgacggta aaacgattgc
      241 gcagcaggtg cgctctgaag ttgctcaaaa agttcaggcg cgtattgcag ccggactgcg
      301 ggcaccagga ctggccgttg tgctggtggg tagtaaccct gcatcgcaaa tttatgtcgc
      361 aagcaaacgc aaggcttgtg aagaagtcgg gttcgtctcc cgctcttatg acctcccgga
      421 aaccaccagc gaagcggagc tgttggagct tatcgatgcg ctgaatgccg acaacaccat
      481 cgatggcatt ctggttcaac tgccgttacc ggcgggtatt gataacgtca aagtgctgga
      541 acgtattcac ccggacaaag acgtagacgg tttccatcct tataacgtcg gtcgcctgtg
      601 ccagcgcgcg ccgcgtctgc gcccctgcac ccctcgcggc atcgtcacgc tgcttgagcg
      661 ttacaacatt gataccttcg gcctcaacgc cgtggtgatt ggcgcatcga atatcgtcgg
      721 tcgtccaatg agcatggaac tgttgctcgc aggttgcacc actaccgtga ctcaccgctt
      781 cactaaaaat ctgcgtcacc acgtagaaaa tgccgatcta ctgatcgttg ccgtcggcaa
      841 gccgggcttt attcctggtg actggatcaa agaaggcgca attgtgattg atgtcggcat
      901 caaccgtctg gaaaatggca aagttgtggg cgacgtcgtg tttgaagacg cggctaaacg
      961 cgcctcatac attacgcctg ttccgggcgg cgttggcccg atgacggttg ccacgctgat
     1021 tgaaaacacg ctacaggcgt gcgttgaata tcatgatcca cagggtgagt aacatggcga
     1081 cattttcttt aggtaaacat ccgcacgttg agctgtgcga cttgctgaaa ctggaaggct
     1141 ggagcgaaag cggcgcgcag gcgaaaatcg cgattgccga aggccaggtg aaagccgacg
     1201 gcgcggttga aacgcgcaaa cgctgcaaaa tcgtcgccgg tcagacagtg agttttgcag
     1261 gtcacagcgt acaggttgtt gcctgatccc tttcccggcc tgtatcaaca ggccggttct
     1321 cttttcagac ttttgccctc tacgccccgt tcatccatgc tgatagcatc gacattttgc
     1381 cgaagcgacc atcatgccaa ccgttattac gcacgccgcc gttcccctat gcattggttt
     1441 agggttgagt tcgaaagtca tccctccgcg tctgttattt gcctgaatca tcctggcgat
     1501 gctgcccgac gccgacgtat tgtcgtttaa atttggcgtt gcttacggca atgtttttgg
     1561 tcatcgcggg tttacccatt cgctggtgtt tgcgtttgtt gtcccgctgt tatgtgtgct
     1621 gattggacgc cgatggttca gggctgggct gattcgctgc tggctgtttt taaccgtctc
     1681 gttgctatcg cacagcatgc tggattcggt aaccactggc ggtaagggtg ttggctggct
     1741 gtggccgtgg tcagatgaac gctttttcgc tccctggcag gtgattaaag tcgcgccgtt
     1801 tgcattgtct tgttacacca cgccgtacgg gcatcaggtg attatttctg aattgatgtg
     1861 ggtgtggttg ccggggatgc tactgatggg aatgttgtgg tggcgcagac gataaccgga
     1921 tgcgaaaact cgcatccggc aatagcgcaa ttacttacga cgccaggtgg tcccttgcgg
     1981 gccatcttcc agaacgatcc ccatctcgtt aagacggtca cgcgccgcat ctgccgccgc
     2041 ccagtctttc gctttacggg catccagacg ctgttgaatt aacgcttcaa tctcagccac
     2101 ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct tccggttctt gctccagcag
     2161 gcctaataca gcggaaagtt tacgcaggtg agatgccatt gcattcgccg ctgccatatc
     2221 ttctgctttc agacggttta cttcacgcgc catatcaaac agtacggaat aggcttccgg
     2281 ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct tcaaacgctt cgccaccggc
     2341 aggagcaacg gttttttctg tgccgcgcag ggcagtgtag agacgctcca gcgccgcacg
     2401 cgcctgcttc aggttctctt cgctgtagtt cagctggctg cgatagtggc ccgacatcag
     2461 gaagtaacgc acggtttcag catcgtagta tttcagcaca tcgcgcacgg taaagaagtt
     2521 acccagcgat ttggacatct tctcgcggtc aaccatcacc attccggagt gcatccagta
     2581 gttaacatac tgaccatcat gggcacaggt ggactgcgcg atttcattct cgtggtgcgg
     2641 gaacatcagg tcagaaccgc cgccgtggat atcaaagtgg ttacccagct gcttgcagtt
     2701 cattgccgaa cattcaatgt gccagcctgg acggcccgcg ccccacggag acggccagct
     2761 cggttcgccc tctttcgaca tcttccacag aacgaagtcc atcgggttgc gtttgtcgtc
     2821 gaccacgtca acgcgcgcgc ctgcctgcag ctggtcgaga tcctgacgcg acagcacacc
     2881 ataagtcgga tcggtcggga cgtcgaacat cacgtcgccg ttgtccgccg cataagcgtg
     2941 acctttggcg atcagttgtt cagtgagttc aataatttct gcgatatggt gcgtcgcgcg
     3001 cggctccata tccgggcgca gaatgttcaa agcatcaaaa tctttgtgca tttcggcgat
     3061 catgcgatcc accagcgcca caaagctttc gccattttca ttggcacgtt tgatgatttt
     3121 gtcgtcgata tcggtaatgt tgcgcacata cttcagttta tagccgagga aacgcagata
     3181 gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga ccaatatgac agagatcgta
     3241 aacggtgatt ccacacacat acatgccgac ttccccggcg tgaataggct taaattcctc
     3301 tttttggcgt gtcagagtat tgaagatttt tagcatcgaa gattccgttt agacatgtgt
     3361 gggtaattga gttgcgtata atacccatat ttcccgcgcg aatcagcata cattgcgtga
     3421 tgatccgatc gtgcggttat gctataacac caccctatat atgacccgaa ctgggttgaa
     3481 gcaccaatca aacggaacag gatgcaaaaa tggttacttt ccacaccaat cacggcgata
     3541 ttgtcatcaa aacttttgac gataaagcac ctgaaacagt taaaaacttc ctggactact
     3601 gccgcgaagg tttttacaac aacaccattt tccaccgtgt tatcaacggc tttatgattc
     3661 agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac caaagaaccg atcaaaaacg
     3721 aagccaacaa cggtctgaaa aatacccgtg gtacgctggc aatggcccgt actcaggctc
     3781 cgcactctgc aactgcacag ttcttcatca acgtggttga taacgacttc ctgaacttct
     3841 ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc tgaagtggtt gaaggcatgg
     3901 acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtag cggtatgcac caggacgtgc
     3961 caaaagaaga cgttatcatt gaaagcgtga ccgttagcga gtaatcgtgg cgacactctt
     4021 tattgcagat cttcatctct gcgtggaaga accggcgatc accgccggtt ttctgcgttt
     4081 tttagcgggg gaagctcgca aggccgacgc gctgtatatt cttggcgatc tgtttgaagc
     4141 gtggattggc gacgacgagc ccaatccact ccatcgccag atggcggcag cgatcaaagc
     4201 ggtgtccgat tctggcgttc cctgttattt cattcatggc aaccgtgatt ttctgctcgg
     4261 caaacgcttt gcccgtgaaa gtggcatgac gttattgccg gaagaaaagg tgctcgaact
     4321 ttatggccgc cgggtgttga ttatgcatgg cgacacgctg tgcaccgatg acgcgggtta
     4381 tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa atgctattcc tcgccctgcc
     4441 gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgagcg aacagtaaag aagccaacag
     4501 cagtaaatcg ctggcgatca tggacgttaa ccaaaacgcg gtagtcagtg cgatggaaaa
     4561 acatcaggtg caatggctga tccacggaca tacccatcgc ccggcggtac atgaacttat
     4621 cgccaaccag caacctgctt ttcgcgtggt actcggtgcc tggcatacgg aaggttcgat
     4681 ggtgaaagtc acggcggatg acgttgagct gattcatttt ccgttttaaa aaacccgcaa
     4741 ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc tctttccgtg ctattctctg
     4801 tgccctctaa agccgagagt tgtgcaccac aggagtttta agacgcatgt cttcccgcaa
     4861 taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc gactgggcta ccatgcagtt
     4921 cgccgccgaa atcttcgaaa tcctgaatgt cctgcaccac gttgaagtgg tttctgctca
     4981 ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc gaagagaacg gttatcaggt
     5041 gattattgcg ggcgcaggtg gcgcagcgca cctgccaggc atgattgccg ccaaaacgct
     5101 ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg agcggtgtcg atagcctcta
     5161 ctccatcgta caaatgccgc gcggcattcc ggtgggtacg ctggcgattg gtaaagctgg
     5221 cgcggcaaac gcggcattac tggcagcaca aattctcgcg actcacgata aagagctaca
     5281 ccagcgtctg aatgactggc gcaaagcaca aactgatgaa gtgctggaaa acccggaccc
     5341 gcgaggtacg gcatgaaaca ggtttgcgtc ctcggtaacg ggcagttagg ccgtatgctg
     5401 cgtcaggcag gcgaaccgtt aggcattgct gtctggccag tcgggctgga cgctgaaccg
     5461 gcggcggtgc cttttcaaca aagcgtgatt accgctgaga tcgaacgctg gccggaaacc
     5521 gcattaaccc gcgagctggc gcgccatccg gcctttgtga accgcgatgt gttcccgatt
     5581 attgctgacc gtctgactca gaagcagctt ttcgataagc tccacctgcc gaccgcgccg
     5641 tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt ttgatcgttt aggtgaactg
     5701 gcgattgtta agcgtcgcac tggtggctat gacggtcgcg gtcaatggcg tttacgcgcc
     5761 aatgaaaccg aacagttacc ggcagagtgt tacggcgaat gtattgtcga gcagggcatt
     5821 aacttctctg gtgaagtgtc gctggttggc gcgcgcggct ttgatggcag caccgtgttt
     5881 tatacgctga cgcataacct gcatcaggac ggtattttgc gcaccagcgt cgcttttccg
     5941 caggccaacg cacagcagca ggcgcaagcc gaagagatgc tgtcggcgat tatgcaggag
     6001 ctgggctatg tgggcgtgat ggcgatggag tgttttgtca ccccgcaagg tctgctgatc
     6061 aacgaactgg ctccgcgtgt gcataacagc ggtcactgga cacaaaatgg tgccagcatc
     6121 agccagtttg agctgcatct gcgggcgatt accgatctgc cgttaccgca accagtagtg
     6181 aataatccgt cggtgatgat caatctgatt ggtagcgatg tgaattatga ctggctgaaa
     6241 ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc gtccggggcg taaagtgggg
     6301 catctgaatt tgaccgacag cgacacatcg cgtctgactg cgacgctgga agccttaatc
     6361 ccgctgctgc cgccagagta tgccagcggc gtgatttggg cgcagagtaa gttcagttaa
     6421 gcaggtgctc atttgattgc cggatgcggc gtaaacgcct tatccggctt accgctccgg
     6481 tacccattgt aggcctgata agacgcgtca agcgtcgcat caggcatggt gcaccgattg
     6541 ccggatgctc tcatcactac agcgaaatac aggtccccgc ttcgcccgcc agcgtctctt
     6601 caattcgcga taacgcccca atccacgcgg gtttaccacg gcttctgaca taaccactca
     6661 ccgccgttac cttcggcccc atcgaaccat cggctttggc aaatggcgct aactcatccg
     6721 gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca gttttcatat accgcatcag
     6781 catcggtgag gatcaccagt ccatcggcat taatctgctc ggcgagcaac gcagcggcga
     6841 gatctttatc aatcactgct tcactccctg ccccatcttc cgtcacaggc acacctccgc
     6901 cgccactgca aatcaccaca tgcccctctt tgagcaacaa ctcgatggct tcgctgtcga
     6961 gaattttacg cggttgcgga gacgccacca cccggcgcaa atatttacca tcacgtttca
     7021 tctgccagcc gtaagccgct tccagtgcct cttgttcttc tggctgataa accggaccaa
     7081 taaatttctc tggctgcaaa aacgccggat catcaggcga aacctcaatg cgcgtcagca
     7141 ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct ctgcgccagc atatagccaa
     7201 tcatcccctg gctttccgcc accagcacat ccagcggata cggttctacc tctttccacg
     7261 ccagattctg taacgccagc agccccacct gcggcccgtt gccgtgaaca atcgccagcc
     7321 gataagaacg ggccaggcgt gccagcgcgg gtacagcact ggcgatattg cgatattgat
     7381 tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt gcccccaaga gctacaacca
     7441 gtgttttcat ggttttcctt gtaataattg ttggccaagc cagaacccca gcagcgtatc
     7501 tgcccctgac gtatgcccca gcgccagcag cgaatcaatc gcaacagcgg tacgtttcgg
     7561 gcaactcaga gcatggacaa agtgcaggag tggcgaggcg aaatatcctt gcgcggcata
     7621 acgtaaataa ctgacgctca ccgctgtggt aacgagttga agattgtcgg aataggcaaa
     7681 aaactggcgt aactcagcag gcagcggatc gcctgccgcc aacgccagtg gcccgaaaag
     7741 cccggtttcc tccgcgcgtt gcatccatgc agctgcaagc ggttgaggat gcgcaggcgg
     7801 cgtaatacgc agcaaacaat aacgctgtgg ctgtttaacc gtgaaacgcc cgaggcgaat
     7861 cccttgagcc acaacctgtg gtcgttcatt gccgcataat ccaccgcata acgcatcgaa
     7921 ttgcgcgcgg cgaagcaccc atcctccggg gccgaaacca ctaccctgac gatgcagcgt
     7981 taacagttcg ccgctttccg tcatcaggtt aatcgcccgt cgccacactc ccgctaaccg
     8041 ccaggactgg cgataattcg gtgcactacg gctggcaagc agaggatgga tgatcgtcat
     8101 tgcgcgttcc tcaaccaatc cccatgcttt cagccagtgc ttccagcgcc tgttcaaaac
     8161 acgccagcgg tgcccgcaca gtgcctgcgc caatctgccc gatccccgcc tctttatggg
     8221 cgataccggt attgatgagt ggcgtaatgt cggtttctac cacgcgacga atgtccagtc
     8281 ccaggcacgc gccctgaaaa tcccagcctg ggatctgcaa ctgcatattg cgctcaaggt
     8341 aaatttccgc catctcttca gataccgctc tggccgcttc catgccaccc gcaccgacaa
     8401 agcgcgttac gccaggcgct gcgatcattg ccgcacctcc gataccaaag gtttcggtaa
     8461 tcgcgctatc gcccatatcc gggttcgcct gctcctgcga gaagccggtg aaaaacagac
     8521 cttgcggagt gtttacaggc gcagtaaacc agcgttcgcc cagcccgctt acccgaatcc
     8581 cgaacatatt gccgttgcgg gtcattgccg tgacgatgct gcctgcgcgg atcatcgcgc
     8641 cagcatccat cgccgccttg cagtaagcca tcgcgaggtt gaggaagaac tgatcggtca
     8701 cgctgaggaa atccatcact tcggcgatgt gctgtttatc atgatcgagg cgagcaattt
     8761 gtggggccag cgcacgcatt aacagtgcag aggaagcaat attgcgttga tggaactcat
     8821 cgcccatcgt aatgccctgc gccatcatcg ccgtgagatc gataccgcgc tccatgcgcc
     8881 ccagcgccgc gcttaatact ggcattaaca catcgcgcat ccagcgatgg cgagtcagga
     8941 catcttcgcc gtaagcgcca aaacgcatca ctttgccgat accttcgttg aggttgcagt
     9001 acgcccggtt gccgtcggtc acgttctcga ccaccagcat cggcatactg gcagaagtaa
     9061 taccgcccat tggaccgacg gcattcacat ggtgacaagg aatgaagttc acttccccct
     9121 gctccagtat tgccagcgcc tgcgcttcat ctttcgccca gccttcgaac agacatgcgc
     9181 ccacgcacgc ccctttcatg ggtccggtca tctcctgcca gcgcattggc ggcccggcgt
     9241 gaagcagtgt tttgccctcg tttagttcgc tgataagtga agaagccggt tgcacatcca
     9301 gccagtgtgg acgagcgcga cgaatttgtt cgataaccgc agcattggct tgcgccactg
     9361 atgtaaacat gggaacccct tattgcaaac gttctaataa acgagccagg taatgactcc
     9421 aacttattga tag
//
LOCUS       DAJVRF010000145         9420 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJVRF010000145 DAJVRF010000000
VERSION     DAJVRF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.145"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(4..930)
                     /gene="glsB"
                     /locus_tag="OQN13_003341"
     CDS             complement(4..930)
                     /gene="glsB"
                     /locus_tag="OQN13_003341"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCS1616618.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(994..2382)
                     /gene="sad"
                     /locus_tag="OQN13_003342"
     CDS             complement(994..2382)
                     /gene="sad"
                     /locus_tag="OQN13_003342"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HCS1616619.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2483..3364
                     /locus_tag="OQN13_003343"
     CDS             2483..3364
                     /locus_tag="OQN13_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1616620.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            3441..4556
                     /gene="yneK"
                     /locus_tag="OQN13_003344"
     CDS             3441..4556
                     /gene="yneK"
                     /locus_tag="OQN13_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCS1616621.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            4706..5896
                     /gene="ydeA"
                     /locus_tag="OQN13_003345"
     CDS             4706..5896
                     /gene="ydeA"
                     /locus_tag="OQN13_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCS1616622.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(5921..6586)
                     /gene="marC"
                     /locus_tag="OQN13_003346"
     CDS             complement(5921..6586)
                     /gene="marC"
                     /locus_tag="OQN13_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCS1616623.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            6798..7232
                     /gene="marR"
                     /locus_tag="OQN13_003347"
     CDS             6798..7232
                     /gene="marR"
                     /locus_tag="OQN13_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCS1616624.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            7252..7635
                     /gene="marA"
                     /locus_tag="OQN13_003348"
     CDS             7252..7635
                     /gene="marA"
                     /locus_tag="OQN13_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCS1616625.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            7667..7885
                     /gene="marB"
                     /locus_tag="OQN13_003349"
     CDS             7667..7885
                     /gene="marB"
                     /locus_tag="OQN13_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCS1616626.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(7942..9381)
                     /locus_tag="OQN13_003350"
     CDS             complement(7942..9381)
                     /locus_tag="OQN13_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCS1616627.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         2425 a         2419 c         2118 g         2458 t
ORIGIN      
        1 gcattaataa accgaacgtc ctaactgttt cgtcaattgt tcaaggacgg cgatacccgc
       61 aagcgagtta cctgcatcat ccagttccgg actccagact gcgatggcca tttcatgcgg
      121 aacaatcgcc acaataccgc caccaacgcc agatttcgcc ggtagtccca cccgccaggc
      181 aaactccccc gcgttctggt acataccact ggtcgccatc agcgcgttaa tttgccgcgc
      241 ctgcattggc gtcactacag gttcatcaat atgaatagct ttcccctgat tagccagaaa
      301 gacaaacgtc cgagccagct ctacacagct cattttcaga gcgcagtaat gaaagtagtt
      361 ttgcagaacg gttgtcacgt catgatggaa attgccaaac gacttcatca gccaggcgat
      421 agccgcattt cgcgcggaat gttcaaattc ggaacgcgct accaccgtat cgtatgaaat
      481 atcagacaca ccgcttaagc cgcgcacgac ttccagcata cgttgccgtg gtgcgcttaa
      541 tcgcccttgc aacatatcgc agaccaccag cgcaccagca ttaataaacg gattacgcgg
      601 aataccctgt tccatttcca gttgcactaa agaattgaac ggtgatccag acggatcttt
      661 gccgacgcgt tgccagattt cctcttcgga gtaatgacgc atggcgacaa cgagactcag
      721 cactttggaa atagactgaa tggaaaaacg ttcttgcgcg tctccggcct gaaaaagctg
      781 tccgtcaacg gtacagatag caatgcccaa tcgggaaccg tctactgtag ccagcgccgg
      841 aatataatcc gcgactttac cctgaccaat gagcggccgc acttgccgca agatgttttc
      901 taaaattgca ttatccatgg cgactgccac tttctactcc tggaccgcag gtctgaaaag
      961 acctgcgagt atatcagagc tgaatatgtc gcgtcagatc cggtctttcc acaccgtctg
     1021 gatattacag aattcgtgta agccgaaatg ggaaagctca cgaccaaagc cactcttttt
     1081 cacgccacca aaggccactc gcgcgtcgct ggcacaataa ccattgataa acaccccacc
     1141 gcattccaga cgtgccgcca tctgtctggc ctgtgtttcg tcggtggtaa aaatggtcgc
     1201 tgaaaggccg aactcactat cattagccag ttccagcgca tgttctgcat ctttcgcaac
     1261 ggtgattgcc gcaacggggc caaacatttc ttcccgaaac gcggtcattt ctggggtaac
     1321 attcgccaga accgttggcg gatagtagtt acctgcccca gccatctttt ccccgcccag
     1381 taacaaacgc gcaccctgcg ccagggtttt ctctacctga tgatgcagct catcacgtaa
     1441 atcaaaacga gccattggtc cgagagtgtt ctcttcgtca cggggatcgc ccattttcaa
     1501 ggctgccgca gctgccacaa aacgttcggt aaatgcagaa gcaattccct cttcgataat
     1561 aaagcgtttt gccgctgcac atacctgtcc ggtattctga taacgtccgg ctaccgccgc
     1621 tttaaccgcc agttccagat cggcatcgtt aagcacaata aacggatccg aaccgcccag
     1681 ttccagtacg cattttttca gtgccgctcc agcctgtgcg ccaatagccg ctccagcacg
     1741 aacacttccg gtcaccgtga cagcagcaat gcgcgagtct ttaatcatct gactgacacc
     1801 gtcgttgtcg gcattcagcc agccatatac gccttgcggg attcccgcat ctttaaacac
     1861 ctgggcaatg agctgagcac atcccatcac attcggcgca tgtttaagta agtagccgtt
     1921 acctgcaaga atgatgggaa cagcgccacg catcacctgc cataacggaa aattccacgg
     1981 cataatcgcc agaatcgtcc ccaacggtcg atactcaata accgcctgct gattttccac
     2041 cagtgtaggt tccgccttca gcattgccgg accatgttct gcataccagt cacacaaatt
     2101 cgccgatttc gccacttcag cgcgcgcctg gttgattggt ttgcccattt cgcgggtgat
     2161 catttgcgcc atttcttcgc tacgggcgcg cagagcctta ccgatatcac gcattttttc
     2221 agcacgataa tctatatttg tctcgcgcca gtcgcgaaag cctgctgccg ccagctcaag
     2281 tgcgttttcg atatcgttag cgccagccca cggcagcaca gaaagttgtt cacccgtggc
     2341 aggatttatt gaaattgcat gagttgccgg agtaatggtc atcggggtat ctcctttatg
     2401 agtcatggta tgaagatacg cagatttact cttgctttaa aatgaataat attaagccac
     2461 ttattcacga atcgagaatg ttatggatct gacccaactg gagatgttca acgcggttgc
     2521 cgaagctggc agcattaccc aggctgcagc aaaagtgcat cgcgtaccgt cgaatctcac
     2581 tacccgtcta cgccagctgg aaacagaact gggggtagat ctgtttattc gcgagaatca
     2641 gcgtttacgt ctctctcctg ccgggcataa ctttttacgc tatagccaac aaattctcgc
     2701 gttagtggat gaagcgcgga gcgttgtcgc tggcgatgag ccgcaaggtt tattttctct
     2761 tggttcgctg gaaagcaccg cagcagtgcg cattccggcc acgctggcgg aattcaacca
     2821 tcgttatccc aaaattcagt tttcactttc caccggccct tccggcacta tgctggaagg
     2881 agtgctggag ggaaaactga atgcggcatt tattgatggg cccattaacc atactgccat
     2941 tgacgggata ccggtatacc gcgaggagct gatgatcgtc acgccacaag gtcatgcgcc
     3001 agtaatccgc gccagtcagg ttaacggcag taacatttat gccttccgca ccaattgttc
     3061 gtatcgtcgc cacttcgaga gctggtttca tgctgacggt gccgctccgg gaactattca
     3121 cgagatggag tcgtatcacg ggatgttggc ctgtgtggtc gcaggagcag gcattgcgct
     3181 tattccacgc tctatgctgg aaagtatgcc ggggcatcac caggttgaag cgtggccgtt
     3241 agctgagcaa tggcgttggt taacaacctg gctggtctgg cgtcgcggtg cgaaaacgcg
     3301 tccgcttgag gcatttattc aactactgga tgtgtctgac tcggcaaaac agagttacca
     3361 atgaactatt ttgataattc ttgcggctaa tatgctctat atagtgatgt tccgatgact
     3421 tatgactata tggggcaaat atggttacgc cagtaagcat cagcaattac atatctcttc
     3481 ctgatgattt tcccgttcgt aatattgcac cacaggtaaa agaagtttta aaggacttta
     3541 ttgatgcgct aagtacaata atatgtgatg aagagtggcg cacgtcttta aacatcaact
     3601 cagccacaaa aaagatattt aataaccttg ataacttatc atatattcag aggacttctt
     3661 tccggggtaa cgacacgcta tacaatgaaa aggttcagtt taaactcact tatcccgtca
     3721 agaatggaag acacaaagaa aatattgaat ttcaggtagt aataaattta agccctattt
     3781 atttagataa ttttcgccat gatggagaaa ttaatatttt ttgcgctccc aacccaaagc
     3841 ctgtcactat ggggcgcgta tttcagaccg gcgtcgagcg tgtgctcttt ctgtttctga
     3901 atgattttat tgaacaattt ccaatgttca accctggcgt tccgatcaaa atagcgcata
     3961 caccacatat tgaacccctg cctccggatc accataccgc tgcagattac ttacgccagt
     4021 ttgatttgct tgtcctgaat tttatctctc gcggtaattt tgtcatactc ccccgattat
     4081 ggaataactc tgaggttcac agatggttcg tcaataaaga tcctaacttg atcaccgcca
     4141 ttctcgacat aacggacagc gaattaaaag aggatttatt gcaaagcctg atggattcat
     4201 taggttctaa caaacatgta ctacccgaag tctgcatctg ctttttattc cttttagcag
     4261 aacaagaatc tcctcatttt caggacttgt ttttattttt cgccaatatg ttattgcact
     4321 atcaccaatt tatgaatccc aatgaaagtg atttgaatga cgtgttgatg ccagcatcat
     4381 taagtgatga taaaattatc aagcatatgg cgcgcaggac cctcgaactg tttgtaaaaa
     4441 atgaaacacc gccaaaagtt actcacgaag acctggtgaa aaacaggcct cgctcccctg
     4501 tcagaccacc tatacccgca acctccaaaa cgccagacct ccctgaacgt cattaaaccg
     4561 tgatgttacc gactctctga cgcggaacag aatcagcgtc agagaaacgg aaaacgcgat
     4621 ccagatcaca aatacattgt attcacatca ttaaccgttt taagatcatt tcatcacttt
     4681 ttcgcaactc acccgataat ctgttatgac aacaaacact gtttcccgca aagtggcgtg
     4741 gctacgggtc gttacgctgg cagtcgccgc cttcatcttc aacaccaccg aatttgtccc
     4801 tgttggcctg ctctctgaca ttgcgcaaag ttttcacatg caaaccgctc aggtcggcat
     4861 catgttgacc atttacgcat gggtagtagc gctaatgtca ttgcctttta tgttaatgac
     4921 cagtcaggtt gaacggcgca aattactgat ctgcctgttt gtggtgttta ttgccagcca
     4981 cgtactgtcg tttttatcgt ggagctttac cgttctggtg atcagtcgca ttggtgtggc
     5041 ttttgcacat gcgattttct ggtcgattac ggcgtctctg gcgatccgta tggctccggc
     5101 cgggaagcgg gcacaggcct tgagtttaat tgccaccggt acagcactgg cgatggtctt
     5161 aggtttacct ctcgggcgca ttgtggggca gtatttcggt tggcgaatga ccttcttcgc
     5221 gattggtatt ggggcgctta tcaccctttt gtgcctgatt aagttacttc ccttactgcc
     5281 cagtgagcat tccggttcat tgaaaagcct cccgctattg ttccgccgcc cggcattgat
     5341 gagcatttat ttgttaactg tggtggttgt caccgcccat tacacggcat acagctatat
     5401 cgagcctttt gtgcaaaaca ttgcgggatt cagcgccaac tttgccacgg cattactgtt
     5461 attactcggt ggtgcgggca ttattggcag cgtgattttc ggtaaactgg gtaatcagta
     5521 tgcgtctgcg ttggtgagta cggcgattgc gctgttgctg gtgtgtctgg cactgctgct
     5581 acctgcggcg aacagtgaaa tacacctcgg ggtgctgagt attttctggg ggatcgcgat
     5641 gatgatcatc gggcttggta tgcaggttaa agtgctggcg ctggcaccag atgctaccga
     5701 cgtcgcgatg gcgctattct ccggcatatt taatattgga atcggggcgg gtgcgttggt
     5761 aggtaatcag gtgagtttgc actggtcaat gtcgatgatt ggttatgtgg gcgcggtgcc
     5821 tgcttttgcc gcgttaatat ggtcaatcat tatatttcgc cgctggccag tgacactcga
     5881 agaacagacg caatagttga aaggcccatt cgggcctttt ttaatggtac gttttaatga
     5941 tttccaggat gccgttaata ataaactgca cacccataca taccagcagg aatcccatca
     6001 gacgggagat cgcttcaatg ccacccttgc ccaccagccg cataattgcg ccggagctgc
     6061 gtaggcttcc ccacaaaata accgccacca ggaaaaagat cagcggcggc gcaaccatca
     6121 gtacccaatc agcgaaggtt gaactctgac gcactgtgga cgccgagcta ataatcatcg
     6181 ctatggttcc cggaccagcg gtacttggca ttgctagcgg cacaaaggca atattggcac
     6241 tgggttcatc ttccagctct tccgacttgc ttttcgcctc cggtgaatca atcgctttct
     6301 gttgcggaaa gagcatccga aaaccgataa acgcgacgat taagccgcct gcaattcgca
     6361 gaccgggaat cgaaatgcca aatgtatcca tcaccagttg cccggcgtaa tacgccacca
     6421 tcatgatggc aaatacgtac accgaggcca tcaacgactg acgattacgt tcggcactgt
     6481 tcatgttgcc tgccaggcca agaaatagcg cgacagttgt taatgggtta gctaacggca
     6541 gcaacaccac cagccccagg ccaattgctt taaacaaatc taacattggt ggttgttatc
     6601 ctgtgtatct gggttatcag cgaaaagtat aaggggtaaa caaggataaa gtgtcactct
     6661 ttagctaacc ttgcatcgca ttgaccaaaa cttgaaccga tttagcaaaa cgtggcatcg
     6721 gtcaattcat tcatttgact tatacttgcc tgggcaatat tatcctctgc aactaattac
     6781 ttgccagggc aactaatgtg aaaagtacca gcgatctgtt caatgaaatt attccattgg
     6841 gtcgcttaat ccatatggtt aatcagaaga aagatcgtct gcttaacgag tatctgtctc
     6901 cgctggatat taccgcggca cagtttaagg tgctctgctc tatccgctgc gcggcgtgta
     6961 ttactccggt tgaactgaaa aaagtgttgt cggtcgacct gggagcactg acccgtatgc
     7021 tggatcgcct ggtctgtaaa ggctgggttg aaaggttgcc gaacccgaat gataagcgcg
     7081 gcgtactggt aaaacttacc accagcggcg cggcaatatg cgaacaatgc catcaattag
     7141 ttggccagga cctgcatcaa gaattaacaa aaaacctgac ggcggacgaa gttgcaacac
     7201 ttgagcattt gcttaagaaa gtcctgccgt aaacaaaaaa gaggtatgac gatgtccaga
     7261 cgcaatactg acgctattac cattcatagc attttggact ggatcgagga caacctggaa
     7321 tcgccactgt cactggagaa agtgtcagag cgttcgggtt actccaaatg gcacctgcaa
     7381 cggatgttta aaaaagaaac cggtcattca ttaggccaat acatccgcag ccgtaagatg
     7441 acggaaatcg cgcaaaagct gaaggaaagt aacgagccga tactctatct ggcagaacga
     7501 tatggcttcg agtcgcaaca aactctgacc cgaaccttca aaaattactt tgatgttccg
     7561 ccgcataaat accggatgac caatatgcag ggcgaatcgc gctttttaca tccattaaat
     7621 cattacaata gctagttgat aacgtgacaa cgtcactgag gcaatcatga aaccactttc
     7681 atccgcaata gcagctgcgc ttattctctt ttccgcgcag ggcgttgcgg aacaaaccac
     7741 gcagccagtt gttacttctt gtgccaatgt cgtggttgtt cccccatcgc aggaacaacc
     7801 accgtttgat ttaaatcaca tgggtactgg cagtgataag tcggatgcgc tcggcgtgcc
     7861 ctattataat caacacgcta tgtagttatt gtcaggctct gatcacagag cctgacatct
     7921 ccgtaatatc ttcctcaagt ctcaaatatt ttcgccattg ttggcgatga gatcccgata
     7981 ccagtaaaac gacgctttac gtatccgttc cagcgttccc ttcccttcgt tgtctttatc
     8041 gacataaatc attccgtagc gctttttcat ttcccctgtt ccggcagaaa ccaggtcaat
     8101 gcagccccac ggggtgtagc caattaagtc aacaccatct tcaactacgg cttttttcat
     8161 ttcccgaata tgggaagcaa agtaatcaat gcgatagtga tcgttcaccg tgccgtcagc
     8221 ttgtctctgg tcaaccgcac caaatccatt ttcgacaata aacagcggca actggaaatg
     8281 atcccagaac cagttgagtg aataacgtag ccctgccgga tcaatttgcc atccccattc
     8341 ggatgtatcg atataagggt tgctgaccaa atctcgtggt tcaacataat ccagttgcgg
     8401 gttatcggga gaaaattgtg tcgtaaaaga catgtagtag ctaaaaccga taaagtcgac
     8461 acatccactg gcaagaatcg cgttatcttc tggtgtgata tcgaggttga atcctttcct
     8521 ggcaaagtaa ttcagcatat gttgcggata atatccacga gcatgaacat cagtaaacca
     8581 gtaacgacga tgcatcgctt tcgtggccat catcatatcg ttgggtgcac acgtcagagg
     8641 atagatgggg cacatggcga tcatacagcc aatgttaaat tcaggattga tcgattttcc
     8701 agtctgtacc gccagggcac tggcaactaa ctcgtaatgt accgcctggt acattatttg
     8761 ttcgcgctca ttgatatctt cctctggcga atacaagata ccggaattag taaatggaca
     8821 caggctttcg ctgaaattca cctgattatt gatttcgtta aacgtcatcc agtactttac
     8881 tttttcttta tagcgcgtga agaccgttga tgcgaagcgg ataaaaaagt cgatcagctt
     8941 acggtttcgc cagccaccat attttgtcac cagatgataa ggcatctcaa aatgcgaaag
     9001 cgtcaccaca ggttccattc cttgcttcag gcattcatcg aacagatcat cataaaattg
     9061 taaaccctct tcattcggct cctgttcgtc accttgcgga aagattcgtg tccaggcaat
     9121 ggaagttcga aagcatttga atcccatctc ggcaaataac tgaatatctg ttttatagcg
     9181 atgataaaaa tcaattgctt catgattggg ataattaagc ccgtcgataa cgccttctgt
     9241 cacttcacgc ggcaccccgt gagcgccagc agtcatcaca tcagcgatac tgatgccttt
     9301 tcctccttca ttccagccac cttccaactg atgcgcggct accgcgccac cccataaaaa
     9361 acctttttta aatcctgaca tacacaactc cttaactaaa atagattacc accagacttc
//
LOCUS       DAJVRF010000146         9322 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJVRF010000146 DAJVRF010000000
VERSION     DAJVRF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.146"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(275..1528)
                     /locus_tag="OQN13_003351"
     CDS             complement(275..1528)
                     /locus_tag="OQN13_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1616628.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1635..2528
                     /locus_tag="OQN13_003352"
     CDS             1635..2528
                     /locus_tag="OQN13_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1616629.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2663..3883
                     /gene="mlc"
                     /locus_tag="OQN13_003353"
                     /gene_synonym="dgsA"
     CDS             2663..3883
                     /gene="mlc"
                     /locus_tag="OQN13_003353"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCS1616630.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            4008..4703
                     /gene="bioD"
                     /locus_tag="OQN13_003354"
     CDS             4008..4703
                     /gene="bioD"
                     /locus_tag="OQN13_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS1616631.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4656..5948)
                     /gene="clcB"
                     /locus_tag="OQN13_003355"
     CDS             complement(4656..5948)
                     /gene="clcB"
                     /locus_tag="OQN13_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCS1616632.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6107..6721)
                     /gene="dmsD"
                     /locus_tag="OQN13_003356"
     CDS             complement(6107..6721)
                     /gene="dmsD"
                     /locus_tag="OQN13_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCS1616633.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6764..7618)
                     /locus_tag="OQN13_003357"
     CDS             complement(6764..7618)
                     /locus_tag="OQN13_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCS1616634.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7620..8237)
                     /gene="dmsB"
                     /locus_tag="OQN13_003358"
     CDS             complement(7620..8237)
                     /gene="dmsB"
                     /locus_tag="OQN13_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCS1616635.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8248..>9322)
                     /locus_tag="OQN13_003359"
     CDS             complement(8248..>9322)
                     /locus_tag="OQN13_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCS1616636.1"
                     /translation="RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSK
                     CEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFER
                     KPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIF
                     KKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPA
                     YTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARG
                     IRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILT
                     SHRPSPLAKGNPSHSNLVQIEKV"
BASE COUNT         2425 a         2367 c         2421 g         2109 t
ORIGIN      
        1 acgtattgtg acttaaggga aatttagcta ccaataatag tagtcttgat cgggttaact
       61 attttactac ttacaaagcg gtagaataac tgcgcatcag taataaatga cacacagcaa
      121 aatgaatccg tttatttggg tacttataat cctgatgacg ctagacgcgc tgcgggaatt
      181 ggctggcgct tcgtctattt taggatggct attaacgttg gtttgagcaa gcaataagtc
      241 cggacgtgta gttaccgcag tccgggctta tttttcaggc gtgcagacga cgatgcaaac
      301 gcgtcccgac cagcagagca atgaccagca tcagagcaat aaatgcgccg acgccgttcc
      361 agccatagtt atgccagaaa acaccaccca gcgtcccggc aatactcgac cccagatagt
      421 aactgaacag atacagcgag gaggcctggc ctttagcgcg ttttgcgcgg gggccaatcc
      481 agctgctggc aactgagtgg gctgcgaaga atcctgctga gaagagtaac attccggcaa
      541 agatcagcca cagcgagctg aataaggtca tcagtaaacc aaacagcata acccccgtcg
      601 aaaacaacat caccggaccg cgcccgtagc gggtggtcat ggttccggct ttgggtgagc
      661 tccatgtacc ggtcaaataa gccagcgata ataagccaac cacggcctga ctgacatgcc
      721 agggggaaag catcaaccga tagccgatgt aattaaacag cgtgacgaac gaccccatca
      781 gcaaaaagcc ttctgcgaac aataacggta atccccggtc acgccagtgc agacgaaagt
      841 tgataaataa cgtcttaggg cgcagcgaag tcgggcgaaa atggcgtgat tccggaagga
      901 ttttccagaa catcaacgcc gaggctagcg cgaaacaacc gattgccgcc agagcaattc
      961 gccagttgaa aaagtccgtg aagacaccac taattaagcg tccgctcatg ccgccaattg
     1021 agttgccgct gatatacaac cccatcgaaa aggccacgaa actggggtgg atttcctcgc
     1081 taagataagt catgccaaca gctgccacgc cacttaacga aagcccaatc aaggcgcgca
     1141 taatcaaaat gccgtgccag ctggtcatca ttgtcgaaag taacgtacaa atggaggcca
     1201 acagtagcgc cgtgaccatc actggtttgc gaccaatggc atcggatagg gggccagtaa
     1261 acagcaaacc aatagccaac atcgccgtgg aaatggacag tgaaatacta ctgttcgcgg
     1321 gggttaagcc aaactcctgc gaaagcaccg gaaggatagg ctgcacacaa tagaggagtg
     1381 caaaggttgc cagtccggca gagaacagcg ccagggtgac gcgcataaat tgcggcgtac
     1441 cgcgtttaat aaattgattt ggctgagaaa tgctttgctt gtcagtgtcg cttgccggag
     1501 cgccatcaac agttgtagta cggctcactt gaaatccttg ctaaatatgc ctgtggatca
     1561 ggcttataca taggttagga aaatcgaatt gttctgtcta atatattaat aatctcaaat
     1621 aagatgtttt aaatatgaat attgaacttc gtcatctgcg ttactttgtt gctgttgcgg
     1681 aagagctgca tttcgggcgc gccgctgccc gcctgaatat ttcgcaacca ccgctaagtc
     1741 agcagattca ggcactggag caacaaattg gtgcccgact gctggcacga actaatcgca
     1801 gtgtattgct gacggcagca ggaaaacagt ttcttgcaga tagtcggcaa atcctgtcta
     1861 tggtagatga cgctgccgct cgcgctgaaa gactgcatca gggtgaagcg ggggagttgc
     1921 gcattggttt tacttcgtcc gctcctttta ttcgggcggt gtccgatacg ttatcgctgt
     1981 ttcgccgtga ttatcccgat gtccatttac aaacccgcga aatgaacact cgtgagcaaa
     2041 tcgctccgct cattgaagga acgctggata tgggattgct gcgtaacaca gcgttaccgg
     2101 agacgcttga acacgcagtc atcgtccatg aaccgcttat ggcgatgatc ccgcacgatc
     2161 atcccctggc aaataacccg aatgtaacgc tggctgaact ggcgaaagaa cccttcgtct
     2221 tttttgatcc gcacgtcggg acagggctgt atgacgatat tctcgggctg atgcgacgtt
     2281 accatttgac gcccgtcatc actcaggagg tgggcgaggc aatgaccatc atcggtctgg
     2341 tttccgccgg tctgggtgtt tcaattttgc ctgcttcatt taaacgtgtt cagctcaacg
     2401 aaatgcgctg ggtgccgatt gctgaagagg atgcggtttc tgaaatgtgg ttggtctggc
     2461 cgaaacatca tgaacaaagt ccagcttcgc gtaactttcg tattcatctg ctgaatgctc
     2521 tcaggtgagg gaaatttcag cgaaaaagcc cgaaaaatgt gctgttaatc acatgcctaa
     2581 gtaaaaattt gacgacacgt attgaagtgc ttcaccatag cctacagatt atttcggagc
     2641 gcgaaaatat agggagtatg cggtggttgc tgaaaaccag cttgggcaca ttgatcaaat
     2701 aaagcagacc aacgcgggcg cggtttatcg cctgattgat cagcttggtc cagtctcgcg
     2761 tatcgatctt tcccgtctgg cgcagctggc tcctgccagt atcactaaaa ttgtccgtga
     2821 gatgctcgaa gcacacctgg tgcaagagct ggaaatcaaa gaagcgggga accgtggccg
     2881 tccggcggtg gggctggtgg ttgaaactga agcctggcac tatctttctc tgcgcattag
     2941 tcgcggggag attttccttg ctctgcgcga tctgagcagc aaactggtgg tggaagagtc
     3001 gcaggaactg gcgttaaaag atgacttgcc attgctggat cgtatcattt cccatatcga
     3061 tcagtttttt atccgccacc agaaaaaact tgagcgtcta acttcgattg ccataaccct
     3121 gccgggaatt attgatacgg aaaatggtat tgtacatcgc atgccgttct acgaggatgt
     3181 aaaagagatg ccgctcggcg aggcgctgga gcagcatacc ggcgtaccgg tttatattca
     3241 gcatgatatc agcgcatgga cgatggcaga ggccttgttt ggtgcctcac gcggggcgcg
     3301 cgatgtgatt caggtggtta tcgatcacaa cgtgggggcg ggcgtcatta ccgatggtca
     3361 tctgctacac gcaggcagca gtagtctcgt ggaaataggc cacacacagg tcgacccata
     3421 tggtaaacgc tgttattgcg ggaaccacgg ctgcctcgaa accatcgcca gtgtggacag
     3481 tattcttgag ctggcacagc tgcgtctcaa tcaatccatg agctcgatgt tacatgggca
     3541 accgttaacc gtggactcat tgtgtcaggc ggcattgcgc ggcgatctac tggcaaaaga
     3601 catcattacc ggggtgggcg cgcatgtcgg gcgcattctt gccatcatgg tgaatttatt
     3661 taacccacaa aaaatactga ttggctcacc gttaagtaaa gcggcagata tcctcttccc
     3721 tgtcatctcg gacagcatac gtcagcaggc ccttcctgcg tatagtcagc acattagcgt
     3781 tgagagtact cagttttcta accagggcac gatggcaggc gctgcgctgg taaaagacgc
     3841 gatgtataac ggttctttgt tgattcgtct gttgcagggt taacattttt taactgttct
     3901 accaaaattt gcgctatctc aatttgggcc aggaaagcat aacttagact ttcaaggtta
     3961 attattttcc tagtttatat ttgtgaagca taacggtgga gttagtgatg ctggagcgtt
     4021 tctttattac cggtacagac acttctgtag ggaaaacggt ggtttcccgc gcattgctac
     4081 aagcgttagc ctcccaggga aaaacagttg cgggatataa acccgtagcg aaggggagca
     4141 aagagacacc cgaagggctg cgtaataaag atgccctggt gttgcagagt gtttcaacca
     4201 tcgaactgcc ttatgaagca gttaatccta tcgcgttaag cgaagaagaa agtagcgtgg
     4261 cgcacagttg cccaatcaat tacaccctca tttcaaacgg cctggcaaac ctgaccgata
     4321 aagtcgatca tgtcgtggta gaagggactg gcggctggcg cagtctgatg aatgatttgc
     4381 gtccactctc tgaatgggtg gtgcaggaac agttgccggt gttgatggtt gtcggtattc
     4441 aggaaggttg cattaaccat gcactgctaa cagctcaggc gattgccaac gacgggctgc
     4501 cgctcattgg ctgggtggct aaccgaatca acccaggact ggcgcattat gcggaagtca
     4561 ttgatgtgct gggtaaaaaa cttccggcac cgctcattgg tgaactgcct tatctgccgc
     4621 gcgccgaaca gcgagaactg gggcaataca tccgcttagc tatgctgcgc agtgtgctgg
     4681 cggtagatag agtcacggtg taacgtccgc gaaattaccg acgcaatgac gcaggcaatc
     4741 aataaaccgg ggagtagctg atactccccg gtcatttcac atatcatcaa cgtcgacata
     4801 atcggcgcgt gcgtggttgc cgccaacagt gtcgccatcc cggtcaatcc gagtaaaagc
     4861 gtaatttctt cgccatcagg gaaccataat cccaggctac gaccatacaa catgccaatg
     4921 gcaagaccga taaatagcgt cggtgtaaag acaccaccgg gtgcgccgga accgctactc
     4981 gccagcacgg cacacagttt acaaaggaag atcccggcaa tgatcattaa cagtggtggg
     5041 gcggttaaaa aggattgtac ggtgctatag ccgttgcccc acactgcagg tgtgaacagg
     5101 gaaagcagac ccacgatcaa cccgcctagt gccagttgcc agggcggcgc aagtttgaga
     5161 ctcacaaatc cacgatgaca ggcgttcatt aacgttaaca acagtggtcc gcacagacct
     5221 gccagcacac ctgtactgat aatcagcgca tagtcactag cctgaaccgt tactgagagt
     5281 tgtacgctgt agagtaacgc gtcgctatga ttaataagat tgctaaccag caacgccacg
     5341 acggcggaaa taatcaccgg gccgagagag gccaacatca tagtgccaaa cagcacttcg
     5401 gctataaata aactgccagc aagcggggcg cgataggccg cagccattcc cgccgcggcc
     5461 ccacaggcga tccataattt ccattcctgg cgtggcgtaa aacgttgggc aaaacaggag
     5521 gcggcaaggg cagctaaaag aatcatcgca ccttcgcgac caattgcact gccgctggtt
     5581 actaccagca gcgaggcaag cgatttaacc aggcttgctg cgtaatcgaa ctgtccatcg
     5641 gtttgcaacg cttccatgta atcggtcggc gcatgagggc gttgttgggt aaatttctgc
     5701 cagcccatca gcaacaaacc cgccgccagt ccgccgagcg ccggagttag cagccgtcgc
     5761 cagggggaaa ggtttgtcgc tgcattgacc agactgccgg agtcattatt gaggaacaac
     5821 cactccagta gcagcatcgc atgacgaaac ccggcaacgg caaaggccgc gagaataccg
     5881 acgactgttg cgataagcag acggcggaac atagtgcgca gatccggata gatgtgtaga
     5941 tgatgcatga gcagaaatgg gcaatgaaac acgggcgact attttgcggc aaaagaggcc
     6001 aaccgcaaaa gcagtgcgtg ctttaagaga taaaaaaacg tgacattgta acttattatt
     6061 gccgaatcgc gcgtaatcgt caccatccgg caatattacg gtgatcctat cgaaagagtg
     6121 gtttaaccgc gacaggaatc aacagttgtg attgccattg cgccagcgtt aagcgagcca
     6181 gttcacccag tgcacggtag aaagggtgtt ctgctttttc gataaaaaca tcaagaaaac
     6241 gtgttgacca cggaaaaagg tgccatgcca gcaattcttc gcattctgtc tggcgaccat
     6301 tctccgccaa ccacgcagcc atcaacagca gtgaaccaaa atgatcttcc ggttcgtttt
     6361 gcttcatttc aaactgaatg cctttctcgc gcatccactg acgaagtgcc aatgttgaat
     6421 cgccaaacag cacagattcg cgatccagcc agaccgatcc ccacggcgga gacggcagtg
     6481 cccacgggcc gacaaacaaa cgctgccagg cctgggcgtg agtctcttca cactgtgtct
     6541 gaaaagccgt caccagcggc gctaatgacg cttctggtag aggccactga gtttcccagc
     6601 cgtcactggt gagtaccgca accagaggtg cggcttccgc actctctgga gcgtaataaa
     6661 acagcgcgcc caacacgcgc gccgcgacag aaaaattatc ttgctgtgaa aaatgggtca
     6721 ttcctaacat cctgaaatgt gcgggttgcc ccgcaccaat ttgttaacct gcaatagcca
     6781 ttcctactgt catgtgcagg ccataaaaga gcacacgccc gatcatctca ccaacaagta
     6841 tgaggattag ccccagaaca agtccggcaa cgtgaggttc acggcgacga atgagcggac
     6901 aaagccagca tccaagacct gcgcataaca ataccacacg ccagacctgt aaggaggcgt
     6961 aatctggtac cagtgcgctg gcttgttgca cggatgagtg aatagacgcc agagacaaac
     7021 cttgcagcac aatcaccccg gcacaagcga tcagcgcgag aacgctaatg atggcaaatg
     7081 gtgtggtatt aaatgtaacg cgggctgccc gcagaattgc ggcagcaagt attggaccgc
     7141 tcaacaatac cgtcaggaag aaagccagcg tagtgtaacc gttatgccag gttggcacag
     7201 tgtcgatttg atacacgcag gtcatcatcc agacgaaaat gacgccgagc gccatgctga
     7261 caagtaacca gagtttcccc aacgcttgcg gcattttacc gatgaccgcc accagccacc
     7321 atagtccgcc aacggcaaaa aatatggaac cggcagcaat ctcgttactc agaccagaag
     7381 ctccgattcg attcagcgag ttaaacgccc gcagtggcga tcccaggtgc atgacagagg
     7441 ccataaaccc gatgcccatc aacagccaga ggaaaaacat gccacggaca atacgctgac
     7501 ggtctgcgtc atttttggcg gcgaaccagc caattccgct aacaatcagc gcgcctacta
     7561 cgcattgacc caatacggta aagataacca gtggccactc atgccatcca tttcccattt
     7621 tacacttcct ccggattagc cagataacca gtggtatcgc tggtcgggcg gctgttggcg
     7681 ttaggtttga taacgatatt gggttttgtg aaatgcgcgc gcggcagtgg cgcgacggca
     7741 gccagagtgc cgtgcttctg gcgcagctct tcaattggac cgaactcaag tgcacgcagc
     7801 gggcaggatt cgacacatat gggttgtttc ccctcggcga cgcgcgaata acaaccatcg
     7861 cacttcgtca tgtgcccttt ttcagcattg tactgtggcg cgccgtacgg acaagccatg
     7921 tggcagtagc ggcagccgat acaaacatct tcatcaacca ccacaaagcc atcttcacgc
     7981 ttatgcattg cgccgctggg gcagactttt gtacatgcgg ggtcatcgca atggttacag
     8041 gagatggaga gataataagc gaacacgttc tggtgccaga cgccattatc ttcctgccag
     8101 tcgccgccag catattcata aatacggcgg aaactgactt ccgggccaag atctttaaaa
     8161 tctttgcacg ccagttcgca ggttttacag ccagtacagc ggctggaatc gataaaaaat
     8221 ccatattgtg tggtcacggg ctactcctta aaccttttcg atctggacaa gattgctgtg
     8281 cgacgggttt ccctttgcca gcggtgaagg gcggtgagag gtcagaatat tgatactgcc
     8341 gccgtgatcg acccggtcac caaacatatc cgctttaagc caagcacctt gcccgatggc
     8401 ggtaacgcca ggcagaatac gcggagtcac ttttgcggca atcagcattt ctccattatt
     8461 gttaaatacc cgcacggtat cgccatgacg gataccgcgt gcctgagcat caatggggtt
     8521 gatccacacc tcttgtgggc aggcctgctg taacacatca atattgccgt agctggagtg
     8581 ggtacgcgct ttgtaatgga agcccgttaa ctgcagtgga taggttttcc gcaggggatc
     8641 gtcccagcca tcaaaacctg gggtatacgc aggaagggga tgaataactt catctttttt
     8701 caattcccag gtatccgcaa tcttcgccag tcgttcagaa taaatttcga ttttccccga
     8761 aggtgttttc aacgggtttg cctgtggatc ttcacggaat gcgcggaaag cgacgtagtg
     8821 ttcttccggg cattttttct taaagatccc ggtcgttttc atctcctcgt agtcgggcat
     8881 ctcagggtta cgttccttcg ttttcgcatg gagatatttg atccattcat gctgactgcg
     8941 accttcagta aaggtttgat aaacgtctgg tcctaagcgt ttggcgactt cactcagcat
     9001 ccagtagatg ggtttgcgtt caaattttgc tgaggttgcg ggttgggcga ggatcacata
     9061 gcccatattc cctgcagatt catgagagat aaggtcttct tgctctgttg gcatcaggtc
     9121 gggcaacagg atatcgcaat acttagccga ggccgtcatg aagtggtcaa tgccaacaat
     9181 catctcgcac ttgctgtcat cctgaagcac ctcatgggtg tgattgatgt cgccatgttg
     9241 attgatcaat gtgttactgg cgtagcacca taaaaacttg atggggacat ccagtttttc
     9301 ttttccacga acaccatcac gg
//
LOCUS       DAJVRF010000147         9304 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJVRF010000147 DAJVRF010000000
VERSION     DAJVRF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9304
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.147"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..868
                     /locus_tag="OQN13_003360"
     CDS             <1..868
                     /locus_tag="OQN13_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1616637.1"
                     /translation="LMAGKVFAFSCNVDGGSSIGAGTTSVYVNLDPVIQPGQNLVVDL
                     SQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNVTYPFPLTTNTNVLDI
                     GDKTPMPLPLKLYITPVGAADGVVIKAGEVIARIHMYKIATLGSGNPRNFTWNIISNN
                     SVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSFYLSGATTDSSRQVFA
                     NTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLGLSATYGQTGNKVSAGT
                     VQSVIGVTFIYE"
     gene            1202..3481
                     /gene="ydeP"
                     /locus_tag="OQN13_003361"
     CDS             1202..3481
                     /gene="ydeP"
                     /locus_tag="OQN13_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCS1616638.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3729..3926
                     /gene="safA"
                     /locus_tag="OQN13_003362"
     CDS             3729..3926
                     /gene="safA"
                     /locus_tag="OQN13_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCS1616639.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            4001..4762
                     /gene="ydeO"
                     /locus_tag="OQN13_003363"
     CDS             4001..4762
                     /gene="ydeO"
                     /locus_tag="OQN13_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCS1616640.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
     gene            5163..6845
                     /locus_tag="OQN13_003364"
     CDS             5163..6845
                     /locus_tag="OQN13_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCS1616641.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            6897..8054
                     /locus_tag="OQN13_003365"
     CDS             6897..8054
                     /locus_tag="OQN13_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS1616642.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPLELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(8020..8142)
                     /locus_tag="OQN13_003366"
     CDS             complement(8020..8142)
                     /locus_tag="OQN13_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616643.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8351..>9304
                     /locus_tag="OQN13_003367"
     CDS             8351..>9304
                     /locus_tag="OQN13_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCS1616644.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAF"
BASE COUNT         2717 a         1988 c         1981 g         2618 t
ORIGIN      
        1 attaatggca ggcaaagttt ttgccttcag ttgtaatgtc gatggcgggt caagtattgg
       61 agcaggtaca acctctgttt atgttaatct cgaccctgta atacagccgg gccagaatct
      121 ggttgtagac ttgtctcagc atataagttg ctggaatgat tacggcggct ggtacgacac
      181 tgatcatata aacctggtac aaggctcagc tttcgcaggc tccctacagt catataaagg
      241 atcgctgtac tggaataatg tgacctaccc cttcccgttg acgacaaata ccaacgtatt
      301 ggatattggt gataagacac cgatgccatt accgttaaaa ctctatatta ccccagttgg
      361 ggcggccgat ggagttgtca taaaagcggg ggaagtcatt gcccgtattc atatgtataa
      421 aattgcaaca ttaggaagcg gaaatccgcg taattttacg tggaatatta tttcaaataa
      481 cagtgttgtt atgccaacag gcggctgcac cgttgatagt cgtaatgtca ctgtggatct
      541 cccggacttt cctggaagcg cagaaatacc ccttggcgta tattgctcaa gcgaacaaaa
      601 gctctcattt tatctttcgg gtgccaccac tgacagtagc cgacaagtgt ttgctaacac
      661 agcacctgat gcaacaaaag catcgggagt gggtgtcact ctaatgcgta atggaaaaat
      721 actggctacg ggagaaaatg tctccctggg gacagtaaat aaaagcaaag tacctctggg
      781 tttaagcgca acctatggac aaacaggaaa caaggtttca gccggaaccg tccagtcagt
      841 cattggtgtc acttttatct atgaataatt gtgattttga ccttagtcta tgcgctccat
      901 tcacgaaaat tgcgcagata attattttgt gaaggctatt atcctacacc tgtaagaaaa
      961 tccgcgcaca attgaagcca gagtcttatt ttttatattg aaaaatatgt taatttaaac
     1021 actatgagag atgatgctta acttatcgta tttaatctat tgtttaacga taatagatta
     1081 aaaatagaaa gagagtagtc ccctactctt atatatccat gttggcgata atccctttgt
     1141 atgtactgtg catcatcgct attacaaatc ctaataattc atttccacac aggataagta
     1201 gatgaagaaa aaaattgaat cctaccaggg tgctgcaggt ggttggggtg ctgttaaatc
     1261 cgtagcgaat gcagtacgta agcagatgga tatacgccat gatgttattg ccatgtttga
     1321 catgaataag ccagagggct ttgactgtcc gggttgtgca tggccagatc ctaagcacag
     1381 tgcgtcattc gacatttgtg aaaacggcgc aaaagcaatc gcctgggaag tcacggataa
     1441 gcaggtaaat gcctctttct ttgctgagaa tacggttcaa tcattactta cctggggaga
     1501 tcacgagctt gaggctgcgg ggcgtctcac tcagcctttg aaatatgatg ccgtcagcga
     1561 ctgttacaag ccattaagct ggcaacaagc tttcgacgaa attggcgcac gccttcaaag
     1621 ctatagtgat cccaatcagg ttgaattcta tacttcgggc cgcacttcca atgaagctgc
     1681 ctttctttat cagctttttg cccgtgaata cgggagcaat aactttcccg actgctccaa
     1741 catgtgccat gaaccgacaa gcgtgggttt ggcagcgagt atcggtgtag gtaaagggac
     1801 cgtgttgctg gaagactttg agaagtgcga tttagtcatt tgcattgggc ataaccctgg
     1861 tacaaaccac cctcgcatgc tgacttcgtt gcgcgcttta gtgaaacggg gagcgaaaat
     1921 gatcgccatc aatcctctac aggaacgtgg cctggagcga tttaccgcac cgcaaaaccc
     1981 gtttgaaatg ctgacgaact ctgagactca gttggccagt gcctactaca acgtgcgcat
     2041 tggtggcgat atggcgttgc tcaaggggat gatgcgcctg ttaattgagc gcgatgatgc
     2101 tgcaagtgcc gcaggtcggc cctcattgct tgatgacgaa tttattcaaa cgcataccgt
     2161 cggctttgac gagctacgcc gtgacgttct caattccgag tggaaagata tcgaacgtat
     2221 ttctggacta agtcagacac aaatcgccga actggctgac gcatatgccg ctgccgatcg
     2281 caccattatc tgttacggaa tggggatcac tcagcacgaa catggtaccc agaatgtaca
     2341 gcaactggtc aatctgctgt tgatgaaagg taacattggc aagcctggtg cgggtatctg
     2401 cccactacga ggtcactcta atgtacaggg cgaccgaacc gtcggtatca ccgagaaacc
     2461 gtctgcagag tttctggctc gcctgggtga gcgctatggc ttcaccccac ctcatgcacc
     2521 tggacatgct gcaattgcca gcatgcaagc aatatgcacg gggcaggctc gagcattgat
     2581 ctgcatgggg ggcaactttg cactggcaat gccagatcgg gaagcgagcg ctgtaccgtt
     2641 aacgcaatta gatttggcgg tacacgtagc cactaagctt aaccgctctc atctgctgac
     2701 cgcacggcat agctatattc tgccggttct ggggcgtagc gagattgaca tgcaaaaaag
     2761 cggtgcgcag gcggtaaccg ttgaggattc aatgtcgatg attcatgcct cgcgtggcgt
     2821 gttaaaaccc gccggtgtaa tgctgaaatc agagtgtgca gtggtcgcgg gaatcgcgca
     2881 ggcagcacta ccccacagcg tggtagcctg ggagtatctg gtggaagatt atgatcgcat
     2941 tcgcaatgac attgaagctg tgttaccaga gttcgccgac tataaccagc gcatccgaca
     3001 tcctggaggt tttcacctga taaatgcagc tgctgaaagg cgctggatga cgccgtcagg
     3061 taaggctaat ttcattacca gcaaagggct gttagaagat ccctcttcag cgtttaacag
     3121 taagctggtc atggcgacag tacgtagcca cgatcagtac aacacgacga tttatggtat
     3181 ggatgatcgc tatcgagggg tattcggtca acgagatgtg gtctttatga gtgctaaaca
     3241 agctaaaatt tgccgtgtaa aaaacggcga aagagttaat cttattgcgc ttacgccaga
     3301 cggtaagcgc agctcacgcc gcatggatag attaaaagtg gtcatttacc ctatggctga
     3361 ccgctcactg gtgacctatt ttccagaatc gaatcacatg ctaactcttg ataaccacga
     3421 tccattaagt ggcattcctg gttataaaag tattccggtt gaattagaac catcaaatta
     3481 attcgcctcc tcatttcttc tgctgtcatc cgcacagcag aagaatttct cattgactat
     3541 tatcgcgcaa tttgctcaca ttgattaaat taaaccacac actataagat ataaacctct
     3601 gcctacagct gtaagaaact ccgctcagta ctgaagtgcc agtcctattt cctcttttct
     3661 ccagcctgtt atattaagca tactgattaa cgatttttaa cgttatccgc taaataaaca
     3721 tatttgaaat gtatgcgacc acagtgaaaa acaaaatcac gcaaagagac aactataaag
     3781 aaatcatgtc tgtaattgtg gttgtcttat tactgaccct tacgttgata gccatttttt
     3841 cggcaattga tcagctgggt atttcagaaa tgggtcgcat tgcaagagat cttacacatt
     3901 tcattatcaa tagtttgcaa gactgaaaat aaacagcaaa ttataaatat gaaatgttaa
     3961 aaaagtatcg ataaaaactt tattgtttta aggagataaa atgtcgctcg tttgttctgt
     4021 tatatttatt catcatgcct tcaacgctaa cattttagat aaagattacg ccttctctga
     4081 cggcgagatc ctgatggtag ataacgctgt tcgtacgcat tttgaacctt atgagcggca
     4141 ttttaaagag atcggattta ctgaaaatac cattaaaaaa tatctacaat gcactaacat
     4201 ccagacagtg acggtgcctg ttcctgcgaa gtttttacgt gcttcaaatg taccgactgg
     4261 attgcttaat gaaatgattg cttatcttaa ctcggaagaa cgcaatcatc ataatttttc
     4321 agaacttttg cttttttctt gcctgtctat ttttgccgca tgcaaaggtt tcattacact
     4381 attaactaac ggtgtgctat ccgtttccgg aaaagtgaga aatattgtca acatgaagct
     4441 ggcgcaccca tggaagctga aagatatttg tgactgcctg tacatcagtg aaagcctgtt
     4501 gaagaaaaaa ctaaagcaag agcaaacgac attctcacag attctattag atgccagaat
     4561 gcagcacgca aaaaatttga tacgcgtaga aggttcagtc aataaaattg ccgaacaatg
     4621 tggttatgcc agtacatctt attttattta tgcgttccgc aaacatttcg gcaacagtcc
     4681 gaagagagtt tctaaggagt accgttgtca aagtcacatg ggtatgaata cgggcaacac
     4741 gatgagtgct ttagctattt gattatttgc taacgagtag tcaaccacgc acgctgcgta
     4801 agaattaaat ggggcagcca ttccctgccc cgcgttgttt ttaggcgata tatttattga
     4861 aataaataag tgacatccat cacatattta tgcacttgca taacctgttg cagattattt
     4921 atgatctcaa ttcggcattt tgtcagtaaa atgcaataat ttattaaata tcaataaatt
     4981 agttgtttat cggcgagaaa ttacttaata gaacagaaag taatgtcaac gctttatgga
     5041 ctgttatttc ccttttttta gctaaatctg ctatctcttt atgtgactaa cttcacttac
     5101 atccacttat ttctcttcgt aaaattactt cggaattaag tacaataaga agaggaacat
     5161 ttatgaagtc tgcattaaag aaaagtgtcg taagtacctc gatatctttg atactggcat
     5221 ctggtatggc tgcatttgct gctcatgcgg cagatgatgt aaagctgaaa gcaaccaaaa
     5281 caaacgttgc tttctcagat tttacgccga ctgaatacag taccaaagga aagccaaata
     5341 ttatcgtact aaccatggat gatcttggtt atggacaact tccttttgat aagggatctt
     5401 ttgacccaaa aacaatggaa aatcgtgaag ttgtcgatac ctacaaaata gggatagata
     5461 aagccattga agctgcacaa aaatcaacgc cgacgctcct ttcattaatg gatgaaggcg
     5521 tacgttttac taacggctat gtggcacacg gtgtttccgg cccctcccgt gccgcaataa
     5581 tgaccggtcg agctcccgcc cgctttggtg tctattccaa taccgatgct caggatggta
     5641 ttccgctaac agaaactttc ttgcctgaat tattccagaa tcatggttat tacactgcag
     5701 cagtaggtaa atggcacttg tcaaaaatca gtaatgtgcc ggtaccggaa gataaacaaa
     5761 cgcgtgacta tcatgacaac ttcaccacat tttctgcgga agaatggcaa cctcaaaacc
     5821 gtggctttga ttactttatg ggattccacg ctgcaggaac ggcatattac aactcccctt
     5881 cactgttcaa aaatcgtgaa cgtgtccccg caaaaggtta tatcagcgat cagttaaccg
     5941 atgaggcaat tggcgttgtt gatcgtgcca aaacacttga ccagcctttt atgctttacc
     6001 tggcttataa tgctccgcac ctaccaaatg ataatcctgc accggatcaa tatcagaagc
     6061 aatttaatac cggtagtcaa acagcagata actactacgc ttccgtttat tctgttgatc
     6121 agggtgtaaa acgcattctc gaacaactga agaaaaacgg acagtatgac aatacaatta
     6181 ttctctttac ctccgataat ggtgcggtta tcgatggtcc tctgccgctg aacggggcgc
     6241 aaaaaggcta taagagtcag acctatcctg gcggtactca caccccaatg tttatgtggt
     6301 ggaaaggaaa acttcaaccc ggtaattatg acaagctgat ttccgcaatg gatttctacc
     6361 caacagctct tgatgcagcc gatatcagca ttccaaaaga ccttaagctg gatggtgttt
     6421 ccttgctgcc ctggttgcaa gataagaaac aaggcgcgcc acataaaaat ctgacctgga
     6481 taacctctta ttctcactgg tttgatgagg aaaatattcc attctgggat aattaccaca
     6541 aatttgttcg ccatcagtca gacgattacc cgcataaccc caacactgag gacttaagcc
     6601 aattctctta tacggtgaga aataacgatt attcgcttgt ctatacagta gaaaacaatc
     6661 agttaggtct ctacaaactg acggatctac agcaaaaaga taaccttgcc gccgccaatc
     6721 cgcaggtcgt taaagagatg caaggcgtgg taagagagtt tatcgacagc agccagccac
     6781 cgcttagcga ggtaaatcag gagaagttta acaatatcaa gaaagcacta agcgaagcga
     6841 aataactaaa ccttcatgcg gcggattttt ccgccgcctt attgagcgag atagcgatgc
     6901 acgttacagc caagccctcc agttttcaat gtaatctcaa atgtgattac tgtttttacc
     6961 ttgaaaaaga gtcgcagttt actcatgaaa aatggatgga tgacagcact ctgaaagagt
     7021 tcatcaaaca atatatcgaa gcgtctggca atcaggtcta ttttacctgg caaggcggtg
     7081 aacccactct ggctggcctg gattttttcc gtaaagttat tcactatcaa caacgctatg
     7141 caggccaaaa acgtattttt aatgcattac aaacgaatgg cattttattg aataatgaat
     7201 ggtgtgcctt tctcaaagaa catgaatttc tggttggtat ctcgatcgat ggccccctgg
     7261 agttacatga ccgttacaga cgcagtaatt caggtaacgg tacttttgca aaagtgatag
     7321 cagccatcga gcgtctgaaa tcatatcaag tagagtttaa tacgttaacc gtcattaata
     7381 acgttaatgt ccattaccct cttgaggttt atcatttttt aaaatctatc ggcagtaaac
     7441 atatgcaatt tatcgaattg ctagaaaccg ggacgccgaa tattgatttc agtggtcata
     7501 gtgagaacac attccgtatc attgattttt ctgtgcctcc cacggcttat ggcaagttta
     7561 tgtcaaccat ttttatgcaa tgggttaaaa acgatgtggg tgaaattttc attcgtcagt
     7621 ttgaaagctt tgtcagccgt tttttgggga atgggcatac aagttgtatt ttccaggagt
     7681 cctgcaagga taatctggtt gttgaaagta atggagacat ttacgaatgc gaccattttg
     7741 tctatccaca gtacaaaatt ggaaacatta ataaatctga actcaaaacg atgaacagtg
     7801 tacaactgac agcgcaaaaa aaacggattt cagcgaaatg tcagcaatgt gcatataaac
     7861 ctatctgcaa tggcggttgt cctaagcatc gtattactaa agtaaacaat gagactgttt
     7921 cttatttttg cgaaggttat aaaatccttt tttcaaccat ggtaccttat atgaacgcca
     7981 tggttgagtt agctaagaac agagtaccgc tttaccacat tatggatgtt gcaaaacaaa
     8041 tggagaataa ttaataacgc tctcttttaa ctccattctt agcctccttc ttatgacctt
     8101 aacaaggcac ccgacataaa ttcctgtcgg gtgtttcgtc atcatcatct tcccttgcga
     8161 aaatcctggc ttacgtggta tgcgtcaggg cattagcaat acccttgatt atcaatagca
     8221 taaccataac cataaccact gaaaattaaa aatttctata tatttcatat tgttaaatga
     8281 tacttaaata aatttcatca gtaaatcatt gccatagata atcattggat ggcacaatca
     8341 attttcgcta atgataacca ttcccattac gttttatatg ttgatagcaa agtatctctg
     8401 tctgctgaag cccttttggc tgcgtaagaa taataaaact tcagtgttgt taatcattat
     8461 tatcctcgca atgatccttg gcgtggttaa aattcaggtc tggttaaacg actggaataa
     8521 tgattttttc aatgcgctaa gccagaaaga aaccgataag ctttggcagc ttgttctttg
     8581 gttccctgcc ctgctgggga tctttgtatt aatatccgta aacaaaacat ggttaattaa
     8641 actactgact attcgctggc gcgaatggtt aacagattac taccttaatc gctggtttgc
     8701 agataaaaac tactacttca cgcagatcta cggcgagcat aaaaatacag ataaccctga
     8761 ccaacgtatc gctgaagaca ttctcttact gatcagcaaa acgctcagtc tttcatttgg
     8821 cttcatccag tcacttagca tgctgatcac ctttaccgtt attctttggc aaagcgcggg
     8881 tacgctctca ttcactgttg gtggaacaga atggaatatc caggggtata tggtctatac
     8941 cgtcgtgctc attgtgatcg gtggaacttt atttacccat aaggtgggta aacgtattcg
     9001 tccgcttaat gtggaaaaac aacgtagcga agcgacattt cgaactaatc ttgtgcagca
     9061 taataagcag acagaactga ttgcgctaag caatgctgag tctctacaac gccaggagtt
     9121 gcgcgacaat tttcatacca ttaaagagaa ctggcatcgt ttaatgaatc gtcagcggtg
     9181 gcttgattac tggcaaaata tttattcgcg ctcgcttagc gttcttccct actttctgtt
     9241 attgccgcag tttattagcg ggcagatcaa tctgggcgga ctgatgaaat cgcgccaggc
     9301 attt
//
LOCUS       DAJVRF010000148         9188 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJVRF010000148 DAJVRF010000000
VERSION     DAJVRF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.148"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            171..1769
                     /gene="rtcR"
                     /locus_tag="OQN13_003368"
                     /pseudo
     CDS             171..1769
                     /gene="rtcR"
                     /locus_tag="OQN13_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1751..2509)
                     /locus_tag="OQN13_003369"
     CDS             complement(1751..2509)
                     /locus_tag="OQN13_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCS1616645.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="OQN13_003370"
     CDS             complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="OQN13_003370"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCS1616646.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="OQN13_003371"
     CDS             complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="OQN13_003371"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCS1616647.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3917..5422
                     /gene="glpD"
                     /locus_tag="OQN13_003372"
     CDS             3917..5422
                     /gene="glpD"
                     /locus_tag="OQN13_003372"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCS1616648.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5474..6079)
                     /locus_tag="OQN13_003373"
     CDS             complement(5474..6079)
                     /locus_tag="OQN13_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616649.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6082..6849)
                     /locus_tag="OQN13_003374"
     CDS             complement(6082..6849)
                     /locus_tag="OQN13_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616650.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6852..7562)
                     /locus_tag="OQN13_003375"
     CDS             complement(6852..7562)
                     /locus_tag="OQN13_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616651.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7578..9083)
                     /locus_tag="OQN13_003376"
     CDS             complement(7578..9083)
                     /locus_tag="OQN13_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCS1616652.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENIR
                     NGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT         2372 a         2358 c         2262 g         2196 t
ORIGIN      
        1 ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt
       61 ttttaattca ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac
      121 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
      181 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      241 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      301 tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      361 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      421 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      481 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      541 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      601 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      661 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      721 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      781 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      841 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      901 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      961 aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc gccaacggcg
     1021 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
     1081 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1141 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1201 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1261 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1321 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1381 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1441 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1501 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1561 aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgctagg
     1621 caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca
     1681 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1741 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1801 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1861 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1921 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1981 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     2041 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     2101 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2161 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2221 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2281 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2341 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2401 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2461 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2521 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2581 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2641 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2701 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2761 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2821 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2881 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2941 aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag ccataacatc
     3001 acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc gatcatcatc
     3061 acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     3121 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3181 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3241 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3301 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3361 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3421 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3481 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3541 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3601 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3661 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3721 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3781 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3841 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3901 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3961 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     4021 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     4081 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4141 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4201 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
     4261 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
     4321 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4381 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4441 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4501 tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4561 cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4621 ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat
     4681 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
     4741 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
     4801 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
     4861 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4921 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
     4981 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
     5041 cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     5101 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     5161 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5221 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5281 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5341 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5401 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5461 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5521 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5581 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5641 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5701 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5761 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5821 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5881 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5941 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagtacg gctaagacaa
     6001 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     6061 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6121 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6181 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6241 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6301 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6361 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6421 catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata
     6481 ctggcaggca aatcgctgta aaattgcccg ttcatccgca cgccagtttt ctttgttggc
     6541 aaggttgata cggcgcagag aaaaaactac gtgaatattg tcaaaatgcc cctgtgcaac
     6601 aaattccagt aaacgcggca gaaaatagcg gacctcatcg ctattttgcg gatcaggccc
     6661 tgggctacta ttccaggcat tgattaagga atacgggatc gctcgtaacg atgtactccg
     6721 taatgccttt tgttcctgct gactcaggca atattcacaa caaaccacaa atttctgcgg
     6781 cacccgataa cgacgaaagg tgtgataaat ctgctcaatt aatgcctgca tggcggggct
     6841 gggttgcata gttaccccct cgcccgctga tcgcgtgtaa acggttgata gacaaaacga
     6901 gcattcgccg cgttcaggcg aataagggcg ctacgcgaaa gggaaaagct ggggcgaatg
     6961 caactattgc agtctcgaca gctttcctct gcgcaatttt ccactgagta atgacgcgtg
     7021 tagagtttca gttcggcctg cttatcgcga aatccgcgta aactgttatt gttatgaaat
     7081 ttccaggcat ctttaaagac ctgaaagata tagacatcct caatccagga gtcacatttt
     7141 ttaagcgggc gctttgctat tatcacccgc tcatccggat ttagcatcgc cgtaaattgt
     7201 acggtgacgt catttgtcgt gttcaggtca cattgaacat ctggtacatc actgatatag
     7261 gggtcaaatc caaaatcagc accacaggca caaagacggc taatggtggc ggtatcgagg
     7321 gaaaatcccg gccatacctt gctggaggtt aagccaatat ttaatgtgac atcatatcga
     7381 agcaatccct caagaccgtc agcaccgacg aacttttccc cttcatccaa ggcctggtta
     7441 ataagttgct gaacaatgcc ttcatcccag tatgccgatt tgcagttggc aatcgtaata
     7501 tccacgcctg tgaagggatc agtaccggca aggcttgcaa ataatgtatt aactaattcc
     7561 atttttatta tccagcttta aggtagcgtc ttatacatcg ggacataacc tacatcttcc
     7621 accaaactct gtccctgcgg cgttaaaaac cactccaaca atttttgtgt ctctgacgtc
     7681 gtattttccc gcgttaccat aaaggcatcg acgatatagg gatatttgcc gttgcgaatg
     7741 ttttcggcag tcggtgcaat accgttaatc gctagcagtt ttatattttt atcggcgttc
     7801 atttgtgttg cgtaataacg gaaggtgtaa cctattgcgt tgtttgtatt acggtattcg
     7861 gcaacgactt taatcattcc ctccatcata cttgccactt ctgtttcctg cggcgagatc
     7921 atgcggacat ttttcatgac ctgggattgc atcaccgtct ggctaccaga gtcttccggg
     7981 cgttgccagg tctggatctc ctgatcgtta ccgccaacag tacgccagtt ggtaattgca
     8041 ccactgaaga tgtcacgcac ctgttgttca gtcagggaat taaccggatt atccgcattg
     8101 acgatgaaaa caaacgcttc acgggcaaaa ggcgtgtaca gcaaagtaac gcccgattcc
     8161 tcagcgcgtt ttttctgccc accggaaggt tgcgccacaa aaataatatc agcatcacct
     8221 ttaacaatcc tgttatacgc atcgggggta cgagagctct ccagatattc cctggtgtga
     8281 aaatcttccg gtataacgct taaagcataa aatgcagagg cataaattgg gtacgccgcc
     8341 gtagcaccat cgagtcgcgg ccagttttgc gtaaactgaa tttgcggttt accacgcagt
     8401 ggtgtcagtt gattattgag tttatcgggt cgccaggccc aggtatcaat attttcgcgc
     8461 acaccgacac cgggattgag ctgtttatat aaataaccct gataaccagc gacaaaacca
     8521 agcatgagaa ggataaacag gagggaatta cggcaacgta gcccggatgt accggttaaa
     8581 ggatgtctgg ttggccggcc gtaccccagc gaaaaactga tatgcgcgcc aacgggtata
     8641 atgactatcc accagtactc acctccgact actgagacta acaaaacgcc cagccagggg
     8701 agcataagat cagccaacca acccgatccc atctggtgaa attgataatc tgccagatgc
     8761 agcgcgatta tccagccaac caggattaat aaaccgggta ctaaaaaaag acattgtcgc
     8821 cagacggcta tttctctttt ttctggtgca ctatcgcctg cccatactcg ccccgagaaa
     8881 aatagcatta atgcgcccag cgccagcata aaaatgttga gcagaaggta acttgttggc
     8941 gtgataagga tattacaaac ttcaataata ccggcaagtc cgacacccag catagcaaca
     9001 accgccgcca aaaattgcgt cagtatgggg atgccgaaaa aagtcattac cagcgaggac
     9061 aaaatccatt ttctgttttg cattattctt tccattcttt ttgaatggtg aaattatact
     9121 ccccgagtcc ccttgcccct tctggacact tttccgaaat gatggcggaa aaaaacggga
     9181 ccctttgg
//
LOCUS       DAJVRF010000149         9080 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJVRF010000149 DAJVRF010000000
VERSION     DAJVRF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9080
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.149"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..916
                     /gene="maeA"
                     /locus_tag="OQN13_003377"
     CDS             <1..916
                     /gene="maeA"
                     /locus_tag="OQN13_003377"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS1616653.1"
                     /translation="NEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGS
                     AGCGIAEMIIAQTQREGLSEEAARQKVFMVDRFGLLTDKMPNLLPFQTKLVQKRENLS
                     DWDTDSDVLSLLDVVRNVKPDILIGVSGQTGLFTEEIIREMHKHCPRPIVMPLSNPTS
                     RVEATPQDIIAWTEGNALVATGSPFNPVVWKDKIYPIAQCNNAFIFPGIGLGVIASGA
                     SRITDEMLMSASETLAQYSPLVLNGEGLVLPELKDIQKVSRAIAFAVGKMAQQQGVAV
                     KTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1050..2052
                     /gene="adhP"
                     /locus_tag="OQN13_003378"
                     /pseudo
     CDS             1050..2052
                     /gene="adhP"
                     /locus_tag="OQN13_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            2198..2482
                     /locus_tag="OQN13_003379"
     CDS             2198..2482
                     /locus_tag="OQN13_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS1616654.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(2710..3363)
                     /gene="fdnI"
                     /locus_tag="OQN13_003380"
     CDS             complement(2710..3363)
                     /gene="fdnI"
                     /locus_tag="OQN13_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCS1616655.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(3356..4240)
                     /gene="fdnH"
                     /locus_tag="OQN13_003381"
     CDS             complement(3356..4240)
                     /gene="fdnH"
                     /locus_tag="OQN13_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCS1616656.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(4253..7300)
                     /gene="fdnG"
                     /locus_tag="OQN13_003382"
     CDS             complement(4253..7300)
                     /gene="fdnG"
                     /locus_tag="OQN13_003382"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6713..6715),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS1616657.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            7532..8413
                     /gene="yddG"
                     /locus_tag="OQN13_003383"
     CDS             7532..8413
                     /gene="yddG"
                     /locus_tag="OQN13_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCS1616658.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            8672..>9080
                     /locus_tag="OQN13_003384"
     CDS             8672..>9080
                     /locus_tag="OQN13_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCS1616659.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGD"
BASE COUNT         2176 a         2363 c         2298 g         2243 t
ORIGIN      
        1 caatgaaatt tgttctttta acgatgacat tcagggcact gcggcggtaa cagtcggcac
       61 actgatcgca gcaagccgag cggcaggtgg tcagttaagc gagaaaaaaa tcgtcttcct
      121 tggcgcaggt tcagcgggat gcggcattgc cgaaatgatc atcgcccaga cccagcgcga
      181 aggattaagc gaggaagcgg cgcggcagaa agtctttatg gtcgatcgct ttggcctgct
      241 gacggacaag atgccgaacc tgctgccttt ccagaccaaa ctggtgcaga aacgcgaaaa
      301 cctcagtgac tgggataccg acagcgatgt gctgtcactg ctggatgtgg tgcgcaatgt
      361 aaaaccagat attctgattg gcgtctcagg acagaccggg ctgtttacgg aagagatcat
      421 ccgtgagatg cataaacact gtccgcgtcc gatcgtgatg ccgctgtcta acccgacgtc
      481 acgcgtggaa gccacaccgc aggacattat cgcctggacc gaaggtaacg cgctggtcgc
      541 cacgggcagc ccgtttaatc cagtggtatg gaaagataaa atctacccta tcgcccagtg
      601 taacaacgcc tttattttcc cgggcatcgg cctgggggtt attgcttccg gcgcgtcacg
      661 tatcaccgat gagatgctga tgtcggcaag tgaaacgctt gcacagtatt cgccgctggt
      721 gctgaacggc gaaggtctgg tactaccgga actaaaagat attcagaaag tctcccgtgc
      781 aattgcgttt gcggttggca aaatggcgca gcagcaaggc gtggcggtga aaacctctgc
      841 cgaagctttg caacaagcca ttgacgataa tttctggcaa gccgaatacc gcgactaccg
      901 ccgtacctcc atctaagcct gcgcccggta gtgaaggcta ccgggcaatt ttcctctccc
      961 tttttcagat ctcatccata ctgggtagtg gcgaataaat ctcatttgcc tcacctgcta
     1021 tgcagaacat catccgaaaa ggaggaacta tgaaggctgc agttgttacg aaggatcatc
     1081 atgttgacgt tacggataaa acactgcgct cactgaaaca tggcgaagcc ctgctgaaaa
     1141 tggagtgttg tggtgtatgt cataccgatc ttcatgttaa gaatggcgat tttggtgaca
     1201 aaaccgacgt aattctgggc catgaaggca tcggtgtggt ggcagaagtg ggtccaggtg
     1261 tcacctcatt aaaaccaggc gatcgtgcca gcgtggcgtg gttctacgaa ggatgcggtc
     1321 attgcgaata ctgtaacagt ggtaacgaaa cgctctgccg ttcagttaaa aatgccggat
     1381 acagcgttga tggcggaaga gtgcatcgtg gtcgccgatt acgcggtaaa agtgccagat
     1441 ggtctggact cggcggcggc cagcagcatt acctgtgcgg gggtcaccac ctacaaagcc
     1501 gttaagctgt caaaaattcg tcccgggcag tggattgcta tctacggtct tggcggtctg
     1561 ggtaacctcg ccctgcaata cgcaaagaat gtctttaacg ccaaagtgat cgccattgat
     1621 gtcaatgatg agcagttaaa actggcaacc gaaatgggtg cagatttagc gattaactca
     1681 cgcaccgaag acgccgccaa aattgtgcag gagaaaaccg gtggcgctca ctctgcggtg
     1741 gtaacagcag tagctaaagc tgcgtttaac tcggcagttg atgctgtccg tgcaggcggt
     1801 cgtgttgtgg ctgtcggtct gccgccggag tctatgagcc tggatatccc acgtcttgtg
     1861 ctggatggca ttgaagtggt cggttcgctg gtcggcacgc gccaggatct aaccgaagcc
     1921 ttccagtttg ccgccgaagg caaagtggtg ccgaaagtcg ccctgcgtcc gttagcggac
     1981 atcaacacca tctttaccga gatggaagaa ggcaaaatcc gtggccgtat ggtgattgat
     2041 ttccgtcact aagaggtctt tgctgcgact cccatgttcg ggtcgcagca tcgcacactc
     2101 tccaacatga aatggctaaa tggattattg ttgcgtagga aggaacaata atgatagtgt
     2161 tcccagagaa ggaacgctac tgagagtaaa gacactaatg aaaatggcca atcatccccg
     2221 cccgggggac attattcagg aatcactgga cgaacttaat gtcagcctgc gcgagtttgc
     2281 cagagcaatg gaaattgcgc cctcaacggc aagccgattg ctgaccggaa aagcagcttt
     2341 gacgccagaa atggcaatta aactttccgt ggtgatcggc agttcgccgc aaatgtggct
     2401 gaatctgcaa aacgcctgga gtctggcaga ggcagaaaaa acggtggatg tgtcgcgatt
     2461 acgccgtctg gttacgcaat aaaaaatggc accttataaa gcgccatttg aaactgatga
     2521 caaacgcaac attgcctgat gcgctacgct tatcaggcct acacgttcac ttcaatctat
     2581 tgtatttgaa gggttttcat gccggataag gcattcacga cgcatccggg atgaacaaag
     2641 cccacgttgt ctacaatatg aaaatggcgc cttttacagc gccattttca gcttaaagtt
     2701 caaaagagat tatatccctt cttcactctc ttttttcgct tctgccttct cgatttcacg
     2761 ataccagcgc ggatggtgtt tcttcgccca gcgacggctc accttccctt cgatcatccc
     2821 tttaatcgac cctttcaccc agaatgccat atacatatgg atcaggatgg cgtggatcag
     2881 gatgataccc gcagccgcgt ggatcagcag gctgtagcga acaacctgca tcgggaagta
     2941 ctgcgcaaag tacggacgcc agataatcac cccggtcacc agcagcacga aaatcatgct
     3001 catgatcgac cagaacatca tcttttgccc ggcgttgtac ttaccaacat ccgccacttt
     3061 gtgctcattg ccttttaata cttcgacaat gttcaacagc cacggaatat ctttcttatc
     3121 cgggatgttg tgatgcacaa aacgcacaaa cataaacatc agtgcgacga aaatcgcaat
     3181 gccgaagaac gggtgcaaaa tgcgtcccat ctgcggcgta ccgaaggttt gcgtcagcca
     3241 ttgcagcgtc gggaagaaga acgaaatccc ggacagcgcc accaggaaga agcaaatcac
     3301 cacggtccag tgacaggcgc gatcaataaa tttggtgcgc acaatcattt tcgacttact
     3361 catgatgatc ctcctcgtca tcgtccactt ccttattcgg gccaataccg atgtagtgga
     3421 aaatcaaccc ggcaaaagtg gcaataaagc cagccgctgc cagcggtttc aacgcgcctt
     3481 tccacaggct taccgaggtg tcgatcttcg gatctttcgg cagaccgtaa tacagctccg
     3541 gctgatcggc gtgatgcagc acgtacataa cgtgcgtacc accgacccct ttcgggttgt
     3601 agacgccagc atgttcgtaa ccacgcgctt tcagtttcgc cacgcgctct tccgccagct
     3661 ccagcatctc cttcttggtg ccgaagtgga tagccccggt cggacaggtt ttcacacaag
     3721 ccggttcctg gccgacgctg acgcgatcga cgcagagcgt gcatttatat acccggttat
     3781 cctctttgtt gaggcgcgga atattaaacg gacacccggc aatgcagtaa ccacagccga
     3841 tgcagttttc cgactggaaa tcgacaatcc cgttagcgta ctgaatgatt gcaccagcag
     3901 acgggcacgc cttcaggcag ccgggatctt cacagtgcat acagccgtct ttacggatca
     3961 gccactccag cttgccgttc tgttcggttt cgctaaagcg catcaccgtc caggacttgg
     4021 cgctcagatc ggcggggtta tcgtaaaccc cgacgcagtg ccccacttca tcacggatgt
     4081 cgttccactc cgaacacgcc acctgacagg ctttacagcc gatacaggtg gaaacgtcga
     4141 taagttttgc gacttctgct ttgtaatcac gcacctgaga aggcggcgtg atggagttag
     4201 ttgcggacct tttgataata tcctgcgttt ccatagccat ctgttcgccc ccttacgcct
     4261 tctcgatgtt gactaagaac gctttatatt ccggcgtttg cgagtttgca tcaccgacat
     4321 tcggcgtcag agtgttagcg atataacctt tacgcgcgac accctcaaag ccccagtgga
     4381 ttggaatacc caccgtttca acctgctgac cgtttacatt cagcggtttc agacggcgcg
     4441 ttaccaccgc caccgcgcgg ataaagccac gcttgcttga gacagtgaca cgatcgccat
     4501 tattaatgcc tttcgccgcc gccagcgttt cgctgatttc cacaaactgt tccggctgag
     4561 caattgcgtt gagcaatgcg tgcttggtcc aggtgtggaa gtgctcggtc agacgatagg
     4621 tcgtacccac atacgggaac tgctcttttt tacccatccg cagcgcgtct tgttcataca
     4681 gacgaacaac cgggttagac accacgttcg gatgcagcgg gttagtgccc agcggcgttt
     4741 caatcggctc gtagtgttcc gggaacggac cttccgccat tttgttgatg gcaaacaggc
     4801 gtcccatccc ttccggctgc atgataaacg gcccggttgg cgtacccggt gcggcattgc
     4861 cgaagtccgg aatatcgtta cccgtccact tgctgccgtt ccactggatc agcatccgtt
     4921 tcggatccca cggtttacca ttgatatccg ccgaagcacg gttgtagagc acgcgacggt
     4981 tgagcggcca cgcccaggcc catcccagcg tattccccag accggatggg tcggagttat
     5041 cgcggttagc catctggttg ccctgctctg tccagctacc ggtgtagatc cagcaagaag
     5101 atgcggttgt accgtcatca cgcagatgcg caaagctact cagcaactga cctttcttcg
     5161 caatcagcac gccattagcg tcatagagat cttccagcgc atagccgtta ttctctttcg
     5221 ccacttcgtc agattgcggt tcgtgcggct gcttgtagtt ccagctcatc ttcatcagcg
     5281 gttctacgcc tttaccacct tcggcctggt acagctcgcg cagatgatgg tagatacccg
     5341 ccagaatttc gccgtcgtta cgcgcttcgc ccggcgcgtc ctgacctttc cagtgccact
     5401 gcaaccagcg accggagtta gcgatagaac catcttcttc ggcaaagcag gtcgaaggca
     5461 gacggaatac ttcagtctga atagacgccg gatcgacatc gttcgactcg ccgtggttct
     5521 gccagaaggt agaggtttca gtcaccagcg gatcgataac caccatgtac ttcagcttgc
     5581 tcaggcagct caccactttg tttttgtccg ggaaggacgc aaccgggtta aagccctggc
     5641 agaagtaacc ggtgactttg ccttcgtcca tcatgttgaa atacttgatg acgtcgtagg
     5701 tctggtccca cttcggcagc cagtcatagc cccagttgtt ctctttctgc gcggcatcgc
     5761 catagaaaga tttcatcagg ctaacgaaga acttcggata gttgctccag tagttcacct
     5821 gatcagccag cgtcgctttc ggcgtgttcg cttccagata cgactgcaaa tcaacctgtt
     5881 tttctgacgg aagcgtcaga taacctggca ggctggtaga gagcagacct aagtcagtca
     5941 gcccctgaat gttggagtga ccacgcaatg cgttcacgcc gccaccggcc atacccatgt
     6001 taccgagcag caactggatc atcgccatag tacggatgtt ctgcgcaccc acggtgtgct
     6061 gcgtccagcc cagcgcgtac aggaaggtgg ttgtgcgatc cggtgcgctg gtggaggcca
     6121 gcacttcaca cactttcagg aagtcggctt ttggcgtacc gcagatgttt tcaacgacgt
     6181 ccggcgtgta gcgggaaacg tgcgctttca gcaggttcca cacacagcgc ggatgagtca
     6241 gtgtttcatc gcgttttgca tagccgtttt catcgaactg atagttccag gacgacttat
     6301 catactggcg tttttcagcg tcgtagccgc tgaacaggcc gtcttcgaaa gcaaaatcat
     6361 cacgcaccag caggctggcg ttggtgtaat gtttaacgta ttcggcgttg attttgttgt
     6421 tttcgatcag gtagcgcaaa acgccagaca ggaacgtaat gtccgtaccg gaacgaatag
     6481 gtgcgtaaat atccgccaca gaagcggtac gcgtaaaacg aggatcgaca acgatcaagg
     6541 ttgcatcgtt gttgtttttc gcttccatcg cccagcggaa accgacggga tgcgcttcag
     6601 cagcgttacc gcccattacc attacgacgt tagcgttttt gatatccacc cagtggttgg
     6661 tcatcgcacc gcgaccaaat gttggagcaa gacttgctac cgttggtccg tgtcagacgc
     6721 gcgcctggtt gtctaccgcc agcatcccga gggagcgggc aaatttctgg gtcagcatcc
     6781 cggtttcgtt gctggcaccg gaggcacaca gcataccggt agaaagccaa cggtttaccg
     6841 ttacgccctg ctcgttcttt tcaataaagt tagcgtcacg gtcagctttc atcagcttcg
     6901 caatacggga gaatgcttct tcccagctaa tgcgctgcca tttgtcagaa cctggcgcac
     6961 gatattccgg gtagcgcaga cggttttcgc tgttgacgta atccagcaaa ccggcccctt
     7021 ttgggcacag cgcaccacgg cttaccggat gatccgggtc accttcaatg tgataaatcg
     7081 cttctctggc gtttttcgcg ccatcaccca ggctatacat caatagcccg caacctacgg
     7141 aacagtatgt gcaggtgtta cggatctctt tagcgcgtaa taatttgtag tttcgcgcct
     7201 gagccagtgc ttgcttcggg gcaaagccca atgccgctac cgttgttcca gccataccgc
     7261 ccgcgcagat tttaaaaaat tgtctgcgac tgacgtccat tgctttcctc ttttttcagg
     7321 ggtattactg cgcgaggaaa ctaacagtaa aattctgaat ctttttgcgc gaaatcaaaa
     7381 agaacacgtg tatgccacaa aataatccgc ctgcgcaaat taacctacct caatagcggt
     7441 agaaaaacgc accactgcct gacaggccag ttaaaaaaat gctataaaat tcagcttaat
     7501 ttttaacggc aagagagaca aaacagcgag catgacacga caaaaagcaa cgctcatagg
     7561 gctaatagcg atcgtcctgt ggagcacgat ggtaggattg attcgcggtg tcagcgaggg
     7621 gctcggtccg gtcggcggcg cagctgctat ctattcatta agcgggctgc tgttaatctt
     7681 cacggttgga tttccgcgta ttcggcagat tccgaaaggc tatttactcg ccgggagtct
     7741 gttattcgtc agctatgaaa tctgtctggc gctttcctta gggtatgcgg cgacccgtca
     7801 tcaggcgatt gaagtgggta tggtgaacta tctgtggccc agcctgacaa ttctctttgc
     7861 cattctgttt aatggtcaga aaaccaactg gctgattgta ccaggattat tattagccct
     7921 ggtcggcgtc tgctgggtgt taggcggtga gaatgggtta cattatgatg aaatcatcaa
     7981 taatatcacc accagcccat tgagttattt cctggcgttt attggtgcgt ttatctgggc
     8041 agcctattgc acagtaacga ataaatacgc acgcggattt aacggaatta ccgtttttgt
     8101 cctgctaacg ggagcatgtc tgtggattta ctattttctc acgccacaac cagaaatggt
     8161 atttagcacg cccgtcatga ttaaactcat ctctgcggca tttaccttag gatttgctta
     8221 tgctgcatgg aatgtcggta tattgcatgg caatgtcacc ataatggcgg taggctcgta
     8281 ttttacgcct gtactttcct cagcgcttgc agccgtgctg ctcagcgccc cgctgtcgtt
     8341 ctcgttctgg caaggcgcgc taatggtctg cggcggttcc ctgctctgct ggctggcgac
     8401 acgtcgtggt taaaataatt gccgggtcta cggcccggca gttaatcaga taaattccga
     8461 caatattaaa aaataaattt caaataccag taattcacct tatatctcat ttcgacttcg
     8521 atcacatatc gataaataat attatttcat ctatttttct cattgttttt aatcaccatt
     8581 ctattttaaa aacagtatgt catttataaa tgacgccgtt taaaattcgt ttagaaaata
     8641 cgtttaacga taattaaaag gattctgtta aatgaaatta aaaatagttg cggtggttgt
     8701 aactggtttg ttagctgcga acgtagcaca cgctgccgaa gtctataaca aggatggtaa
     8761 taaactcgac ctttatggca aggttaccgc tctacgttat tttactgatg ataagcgtga
     8821 cgatggtgat aaaacttatg cccgtctcgg ctttaaagga gaaacgcaaa tcaatgatca
     8881 aatgattggt tttggtcact gggaatatga ttttaaaggc tataacgatg aagccaacgg
     8941 ctcgcgcggc aacaagaccc gtctggccta tgctggttta aaaattagtg aatttggctc
     9001 tctggactat ggtcgtaact acggtgtcgg ctacgacatt ggttcatgga ccgatatgtt
     9061 gccagaattt ggtggcgata
//
LOCUS       DAJVRF010000150         9034 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJVRF010000150 DAJVRF010000000
VERSION     DAJVRF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9034
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.150"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            73..732
                     /locus_tag="OQN13_003385"
     CDS             73..732
                     /locus_tag="OQN13_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCS1616660.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            836..1810
                     /gene="ghrB"
                     /locus_tag="OQN13_003386"
     CDS             836..1810
                     /gene="ghrB"
                     /locus_tag="OQN13_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCS1616661.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1860..2570)
                     /locus_tag="OQN13_003387"
     CDS             complement(1860..2570)
                     /locus_tag="OQN13_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCS1616662.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            3004..3294
                     /locus_tag="OQN13_003388"
     CDS             3004..3294
                     /locus_tag="OQN13_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCS1616663.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3575..3787
                     /gene="cspA"
                     /locus_tag="OQN13_003389"
     CDS             3575..3787
                     /gene="cspA"
                     /locus_tag="OQN13_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCS1616664.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="OQN13_003390"
     CDS             complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="OQN13_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCS1616665.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="OQN13_003391"
     CDS             complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="OQN13_003391"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCS1616666.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="OQN13_003392"
     CDS             complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="OQN13_003392"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCS1616667.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(7538..7837)
                     /locus_tag="OQN13_003393"
     CDS             complement(7538..7837)
                     /locus_tag="OQN13_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCS1616668.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            8012..9007
                     /gene="wecH"
                     /locus_tag="OQN13_003394"
     CDS             8012..9007
                     /gene="wecH"
                     /locus_tag="OQN13_003394"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCS1616669.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
BASE COUNT         2240 a         2284 c         2235 g         2275 t
ORIGIN      
        1 tcatgttcac gcggcataat ctcccgccac ggaacccgtg gcaaagaata aaaaggttat
       61 taaggattaa caatgaagaa acgtgtttat cttattgccg ccgtagtgag tggcgctctg
      121 gcggtatctg gctgcacaac taacccttac accggcgaac gcgaagcagg caaatctgct
      181 atcggcgcag gtctgggctc tctcgtgggc gcgggtattg gtgcgctctc ttcttcgaag
      241 aaagatcgcg gtaaaggcgc gctgattggc gcagcagcag gcgcagctct gggcggcggc
      301 gtgggttatt acatggatgt gcaggaagcg aagctgcgtg acaaaatgcg cggcactggt
      361 gttagcgtaa cccgcagcgg ggataacatt atcctcaata tgccgaacaa tgtgaccttc
      421 gacagcagca gcgcgaccct gaaaccggca ggcgctaaca ccctgaccgg cgtggcaatg
      481 gtgctgacag agtatccgaa aacggcggtt aacgtgattg gttataccga cagcacgggg
      541 ggtcacgacc tgaacatgcg tctctcccag cagcgtgcgg attccgttgc cagcgcgttg
      601 atcacccagg gcgtggacgc cagccgcatc cgtactcagg gccttggccc ggctaaccca
      661 atcgccagca acagcaccgc agaaggtaag gcgcaaaacc gccgtgtaga aattacctta
      721 agcccgctgt aatccctttc atgccaggtg atgcagaaat cacctggcat tcttcagttt
      781 tccttcatca tatttcaggc taaggtgatc gccttatcag tgaatggaga gaagcatgaa
      841 gccgtccgtt atcctctaca aagccttacc tgatgattta ctgcaacgcc tgcaagagca
      901 tttcaccgtt caccaggtgg caaacctcag cccacaaacc gtcgaacaaa atgcagcaat
      961 ttttgccgaa gctgaaggtt tactgggttc aaacgagaat gttgatgccg cattgctgga
     1021 aaaaatgccg aaactgcatg ccacatcaac gatctccgtc ggctatgaca attttgatgt
     1081 cgatgcgctt accgcccgaa aaattctgct gatgcacacg ccaaccgtat taacagaaac
     1141 cgtcgccgat acgctgatgg cgctggtgtt gtctaccgct cgtcgggttg tggaggtagc
     1201 agaacgggta aaagcaggcg aatggaccgc gagcataggc ccggactggt acggcactga
     1261 cgttcaccat aaaacactgg gcattgtcgg gatgggacgg atcggtatgg cgctggcaca
     1321 acgtgcgcac tttggcttca acatgcccat cctctataac gcgcgccgcc accataaaga
     1381 agcagaagaa cgcttcaacg cccgctactg cgatttggat acactgttac aagagtcaga
     1441 tttcgtttgc ctgatcctgc cgttaactga tgagacgcat catctgtttg gcgcagaaca
     1501 attcgccaaa atgaaatcct ccgccatttt cattaatgcc ggacgtggcc cggtggttga
     1561 cgaaaatgca ctgatcgcag cattacagaa aggggaaatt cacgccgccg ggcttgatgt
     1621 cttcgaacaa gagccactgt ccgtagattc gccgttgctc tcaatggcca acgtcgtcgc
     1681 agtaccgcat attggatctg ccacccatga gacgcgttat ggcatggccg cctgtgccgt
     1741 ggataattta attgatgcgt tacaaggaaa ggttgagaag aactgtgtga atcctcacgt
     1801 cgcggactaa gccgcgactg cgtgaagtaa agcccgataa tcgctcgggc ttttactctt
     1861 tattgggttg cagtaactgc tgtagtccag gcctgattaa acgcctgatg ttgtgccggt
     1921 aatggcgcaa tcagtttgtt atattcactt gcctgctgtg aagtcgggaa ctggatgcca
     1981 tttgcgacaa agctcacctg agtaccttgc tgagcaatgt agtctccaac catcaccagt
     2041 tgttgcgtga aagtttgtgc cgccggaatc aacggttgca atgcatcagc tggcgtcgtt
     2101 accactttgg tgaatgcctg atcaaatacc ggtttcaggt catcgctctg ttttagcgca
     2161 gagtgcgcag catcggcctg caacttcgca ttttgcagtt gttgcgccag cacacccagc
     2221 gaaccattca tttcacgcag cggaccgctt tgcgtaacgt aatcctgcgg tacgcgaatt
     2281 gcgttaacgc tgtctacgac cgggcgcaga ccagaatcca tcgcctgatt cacctgctga
     2341 gaataaccat acagaatcgc gtaatcagag acaaaaggac caaactgttt tttctgatcg
     2401 gcagtcaggg ttggtagacg ttcaccgcta cgcatcactg tattctgcag gaagtcgata
     2461 aacgctttac gctgatcgcc ttctttatca aaacacccac tcaggctaac taccattaat
     2521 aacgccgcaa gaggcgcaaa ccagcgagag caggactttc ctgtcgccat tttcttactc
     2581 ctttcaccca aaaaaagcgc acaacgacac acgcgtgtct gacgtgacaa ggatagtcca
     2641 ggacaggctt gcaggatacc ctttctacgt aatcttcctg gtaaaatcat ggagaaaatg
     2701 aaacaaggtt aatttgttgt ttcattgtta aaaaatgata gtgccgttgc cttttttgta
     2761 gccagagatt tacgccttcg cagaaggtta gattttcgaa atctttacaa aacaggagtg
     2821 gtaagagata tatcggcatt tatcagatcg ttaggtggct ataagtcacg ctaaatgaca
     2881 ggctgaatcg aatcatagcc agagcatgcc ctgacttcac cccgctgtgt ctgcttttcc
     2941 cgactattct taatgagctt cgatgcaatt cacgatcccg cagtgtgatt tgaggagttt
     3001 tcaatggaat ataaagatcc aatgcatgag ctgttgagca gcctggaaca gattgttttt
     3061 aaagatgaaa cgcagaaaat taccctgacg cacagaacaa cgtcctgtac cgaaattgag
     3121 cagttacgaa aagggacagg gttaaaaatc gatgatttcg cccgggtttt gggcgtatca
     3181 gtcgccatgg taaaggaatg ggaatccaga cgcgtgaagc cttcaagtgc cgaactaaaa
     3241 ttgatgcgtt tgattcaagc caacccggca ttaagtaagc agttgatgga atagactttt
     3301 atccacttta ttgctgttta cggtcctgat gacaggaccg ttttccaacc gattaatcat
     3361 aaatatgaaa aataattgtt gcatcacccg ccaatgcgtg gcttaatgca catcaacggt
     3421 ttgacgtaca gaccattaaa gcagtgtagt aaggcaagtc ccttcaagag ttatcgttga
     3481 tacccctcgt agtgcacatt cctttaacgc ttcaaaatct gtaaagcacg ccatatcgcc
     3541 gaaaggcaca cttaattatt aaaggtaata cactatgtcc ggtaaaatga ctggtatcgt
     3601 aaaatggttc aacgctgaca aaggcttcgg cttcatcact cctgacgatg gctctaaaga
     3661 tgtgttcgta cacttctctg ctatccagaa cgatggttac aaatctctgg acgaaggtca
     3721 gaaagtgtcc ttcaccatcg aaagcggcgc taaaggcccg gcagctggta acgtaaccag
     3781 cctgtaatct ctgcttaaaa gcacagaatc taagatccct gccatttggc ggggattttt
     3841 ttatttgttt ttcaggaaat aaataatcga tcgcgtaata aaatttagta ttatttttgt
     3901 gaagaataaa tttgggtgca atgagaatgc gcaacgccgt aagaaaggcg ggaataactt
     3961 cccgccaaag actcttactc tttcaaattg caggctaaaa atgccgccag ctcataactc
     4021 tcctgtttaa tatgcaattc acacagtgaa tctcttatca tccaggtgaa aaataaaagc
     4081 gtgaaacaaa tcactattaa agaaagtaat ctatatttct gcggcattcc cagcctcctg
     4141 tgttgatttc caacgagtaa gaggctaacc ttatggtgtt caggcatagg gtagcctcac
     4201 tttgatttat agtcaggtgg ggcttttctc tgtctgcctt tcggtgaata cctgagacaa
     4261 acagtctcaa gcacccgtgg ctattctagc ttaataagtt tgtttcttct ccttgatata
     4321 atcctaaaaa aacctcataa aattaatata tgagataatc tttattcaac agaagattat
     4381 taaaggttgc tgtattattt agcgataata aaaaaagcct gccagatggc aggcttttta
     4441 ataacggcgt tattactgca acagcgaaat atccgcaacg cgcaggaaca gttcgcgcag
     4501 tttctccagc atggtcagac ggttgagacg caattctttg tcatcaacca tgaccatcac
     4561 tttatcgaag aaagcatcaa ccggctcacg cagctctgcc agttcgacca gcgcatcctg
     4621 gtaacgacct tccgcaaagt acggctccag cttgtcacgt agcaccacaa cctgcatcgc
     4681 cagtttaatt tcttccggct ctttcagggt agaggcattc acgcggtcgc tcagcacttc
     4741 gtcagatttc gccagaatgt tagatacacg tttgttcgcc gccgccagtg cagcagctgc
     4801 atccagggta cggaaatgcg ataccgcttt catacgggca tcgaaatcag ccggacgagt
     4861 cggacgacgc gccagtaccg cctggatggt gtcaacggtg taaccttcgt cctgatacca
     4921 ggcgcggaag cgaccgagca taaagtcgat aacatcatca actacgttgg cgttagtcag
     4981 cttatcgcca tacagacgca ctgcttcttc ggtcagcgtt tgcagatcaa ggttgaggtt
     5041 cttctcaacg ataattcgca gcacgccaag tgcggcacga cgcagcgcaa acgggtcttt
     5101 gtcgcctttc ggatgctgac cgataccgaa gatacccgcc agagtatcca tcttatcagc
     5161 aatcgccagc gcacaagcta ccgggttgga cggcaggtca tcaccggcaa agcgcggctg
     5221 atactgctca ttcagcgcga cggctacgtc ttccgcttcg ccatcgtgac gcgcgtagtg
     5281 catccccata acgccttggg tgtcggtgaa ctcgaagacc atgttggtca tcaggtcaca
     5341 cttagacagc aggcccgcac gggttgcgtg gttaacgtca gcgccaatct gttcagcaat
     5401 ccagccagcc agcgcctgga tgcggtcagt tttgtcacgc agcgtaccca gctgttgctg
     5461 gaacagcacg gtttgcaggc gcggcaggtt atcttccaga cgttttttac ggtcggtatt
     5521 gaagaagaac tcggcatccg ccagacgtgg acgaacgact ttctcgttac cagagataat
     5581 ttgctgcgga tctttcgatt cgatgttggc aacgaagatg aagttcggca gcagtttgcc
     5641 atcgttcgca tacaccggga agtatttctg gtcacctttc atggtgtaaa ccagggcttc
     5701 agccggcacc gcgaggaatt tctcttcgaa ttttgcggtc agaacgaccg gccactccac
     5761 cagcgaagcc acttcttcca gcaggctttc gcttaagtca gcgttaccgc caatcttacg
     5821 cgctgcttct tcggcatcgg ctttaatctt cgctttacgt tcttcgtaat cggcgatgac
     5881 tttcccgcgc tcacgcagaa tttccggata ctgatcggcg ttgtcgatgg tgaattccgg
     5941 ttcgcccata aagcggtggc cgcgaatcac gcgatcggac tgaatgccca gaatggttgc
     6001 cggaatgact ttgtcgccca gcagcagggt cacggtgtgc accggacgca cgaagtgcac
     6061 gtcgcttgcg ccccaacgca tcagtttcgg gatcggcagt ttcgccagcg aagtcgcaac
     6121 catattcggc agtagtgctt cagtgctttc gcccttcaca tgggcgcgat acagcagcca
     6181 ttcgccttta tcggtagtca gacgctcagc ctggtcaacg gtaataccgc aaccacgcgc
     6241 ccaaccttct gccgctttgc tcggtttgcc ttcagcgtcg aacgcctggg caatcgccgg
     6301 gccgcgtttt tcgatttcac gatctggttg cgcttccgcc aggttagcta ctttcaacgc
     6361 cagacgacgc ggagcagcaa accattgaac ggtgccgtgt gcgaggccag cgttatccag
     6421 ctccgcagta aagttcgcag caaaggactc agccaggctg cgcagtgctt ttggtggcag
     6481 ctcttcagtg ccgatttcca ccagaaaagt tttctcagac atagccgcct cttacttatc
     6541 tttgttgcac atcgggaagc cgagggcttc acgggaagcg tagtatgctt ctgccactgc
     6601 tttggtcagg gtgcgaatgc gcagaatgta gcgctgacgc tcagtgacgg agatggcttt
     6661 acgcgcatcc agcaggttga agctgtgggc ggctttcaga atacgctcgt aggctggcag
     6721 cggcagcgga ttttccagcg ccagcagctg ctgcgcttct ttctcgtact gctcgaagca
     6781 ggtgaacagg aaatccacat ccgcgtattc gaagttgtaa gtggactgct ccacttcgtt
     6841 ctgatggaac acgtcgccgt aggtggtttt acccagcggg ccgtcgctcc agaccaggtc
     6901 gtaaacgctg tctacgccct gaatgtacat ggccagacgt tccagaccgt aggtgatctc
     6961 gccggtaacc ggtttacact ccagaccacc aacctgctgg aagtaagtga actgcgtcac
     7021 ttccatgccg tttagccaca cttcccagcc cagtccccag gcacccagag tcgggttttc
     7081 ccagttatct tccacgaaac ggatgtcgtg aatagtcggg tccatgccca gctctttcag
     7141 agaaccgagg tacagctcct gaatattgtc cggcgatggc ttaatgacca cctggaactg
     7201 atagtagtgc tgtaaacggt tggggttttc gccgtagcga ccatcggtcg ggcgacgaga
     7261 aggctgaaca taagcagccg ccatcggttc tggccccagc gcgcgcagac aggtcattgg
     7321 gtgagaggtt cccgcgccga cttccatgtc caatggttga acaatggtgc agccctggcg
     7381 agcccagtaa tcctgtaagg tcaggatcaa gccctggaag gtcctggtat caaacttttg
     7441 catattattt cgtgctggat acgtgtggat ttaaaggaag ggatcagtat acccgctgga
     7501 tggaagatat acagtacgaa acgggaaaaa gcagggctta acgcatggaa agatgcaaaa
     7561 aatggccatc tgcgtcaaaa gaacagacaa actgctcttt acggaaggta taaccgcgca
     7621 tttcatagct tccctggaac tgttcgaagg cggtgacgtc gattttttgc tcgccggtgt
     7681 tataacgttt tgccgcctga tccttgcata acgcttccat attcaacgaa cgcagaggat
     7741 caacctttac ccgttgtgct ttctgcacgg gtgacggtgt agaccaaccc actagcacca
     7801 taagcgccat tgccggaaag aaagcgttca tcatcattaa attaccgtct ggtttgcagg
     7861 tgagtcttat tatttatatc ggtatagtgg ctgatatctt acgaatttcc ttaagcctct
     7921 ataagctgta gtgataaaac tcagattatt ccagtgcgcc aaccaaatga tttatcggtc
     7981 agactttttt taaccagata cagagacaca gatgcagccc aaaatttact ggattgataa
     8041 cctgcgaggg atagcgtgtt taatggtggt gatgattcac accactacct ggtatgtgac
     8101 caatgctcat agtgttagcc ccgtcacatg ggatatcgcc aatgttctga attctgcctc
     8161 tcgtgtcagc gtgccgctat ttttcatgat ttccggctat ctcttttttg gcgaacgcag
     8221 cgcccagccg cgccatttct tgcgtatcgg cttatgtctg attttttata gcgcaatcgc
     8281 actgctctac attgcgctgt ttacctccat caatatggag ttagcgctga aaaacctgct
     8341 gcaaaagcca gtgttttacc acttgtggtt tttcttcgcg attgcggtga tttatctggt
     8401 ttcaccgctg attcaggtga agaacgtcgg cggaaaaatg ttgctggtgc taatggcggt
     8461 gattggcatt atcgctaacc caaacacagt gccgcagaaa attgacggtt ttgaatggct
     8521 gccaattaac ttatatatca atggcgatac tttttactac attctgtatg gcatgttggg
     8581 ccgcgctata gggatgatgg acacacagca taaagcactg tcgtgggtga gcgccgcgct
     8641 gtttgcgacg ggggttttta ttatctctcg cgggacatta tatgaattgc agtggcgcgg
     8701 aaattttgcc gatacctggt atctttactg tgggccgatg gtttttatct gcgcaatcgc
     8761 gctattgact ctggttaaaa acacgctgga tacgcgtacc attcgcggac ttggcttaat
     8821 ctcccgccat tcattgggta tatacggatt ccacgccttg attatccatg cgctgcgcac
     8881 ccggggaatt gagcttaaaa attggccaat actggatatt atttggatct tttgcgcgac
     8941 gttggcagcg agtttgttac tttctatgct ggtacaacga atcgacagaa acagattagt
     9001 gagttaagta aaagcccgtt cacattggac tgac
//
LOCUS       DAJVRF010000151         8934 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJVRF010000151 DAJVRF010000000
VERSION     DAJVRF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8934
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.151"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            225..2201
                     /locus_tag="OQN13_003395"
                     /pseudo
     CDS             225..2201
                     /locus_tag="OQN13_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2194..3330
                     /locus_tag="OQN13_003396"
     CDS             2194..3330
                     /locus_tag="OQN13_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCS1616670.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3327..5327
                     /locus_tag="OQN13_003397"
     CDS             3327..5327
                     /locus_tag="OQN13_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCS1616671.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5449..5910
                     /locus_tag="OQN13_003398"
     CDS             5449..5910
                     /locus_tag="OQN13_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCS1616672.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5951..6421)
                     /locus_tag="OQN13_003399"
     CDS             complement(5951..6421)
                     /locus_tag="OQN13_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCS1616673.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6468..7187)
                     /gene="yehT"
                     /locus_tag="OQN13_003400"
     CDS             complement(6468..7187)
                     /gene="yehT"
                     /locus_tag="OQN13_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCS1616674.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7184..8869)
                     /gene="btsS"
                     /locus_tag="OQN13_003401"
     CDS             complement(7184..8869)
                     /gene="btsS"
                     /locus_tag="OQN13_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCS1616675.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT         2133 a         2385 c         2312 g         2104 t
ORIGIN      
        1 agcggccacg atacgtattg attgaaggcc cggctgattt taatgaccgg gtagacgaac
       61 tgtttttagc ccaccagctt ccggtagcta tttacagtta ttgccagtat caggacggtg
      121 cagcccccgg gcgtggtgcc tggacgccat ttgctgaatt ttcgccggag tggcaggcgc
      181 tacaagccgc acgtcgtatt caggcacaaa cttacttcat cgatttgcct tgctgggcgc
      241 agagtgaaga agtggacgat tcgcctgata cgcaagagga aagccaggcc ttgctgctgc
      301 gtgccacccg catggataac agcgacaccc tgtgggatca cttgttcgaa gatgaaagcc
      361 agcaaactgc attaccctct gcactggcgc actattttgc ccaactgcgg ggcgattccc
      421 ccggcgatgc actcaatcgt cagcgcgaag cctttatggc ccgctggatt ggatgggcga
      481 tgcagcaaaa caatggcgac gtgttagtcg tctgcggtgg ctggcacgct cctgcactgg
      541 caaaaatgtg acgcgaatgc ccgcaggaca ttaacacgcc ggaattgccc tcgctggcag
      601 atgccgttac aggttgttat ctcaccccct acagtgaaaa gcgccttgat gtgctggcag
      661 gatacctttc cggtatgcct gccccggtct ggcaaagctg gtactggcag tggggcttac
      721 agcaggcggg agaacaactg ctaaaaacgg ttcttacccg tttgcgccag cacaaattgc
      781 ctgcttcgac cgcggatatg gctgccgctc atctgcatgc gatggcgctg gcacagttgc
      841 gcggacataa actaccgtta cgcactgact ggctggatgc catagcaggc tcgctgatta
      901 aagaagccct gaacgcgccg ttgccgtgga gctatcgcgg agttattcat cccgacaccg
      961 atccgattct gctaacgttg atagacacat tagcgggtga cggattcggt aaacttgccc
     1021 cttctacgcc acaaccgcct ctgccaaaag atgtcacctg cgaactggaa cgtaccgcaa
     1081 tctctcttcc ggcggagctt accttaaatc gctttaaccc caatgggcta gcgcaaagtc
     1141 aggtgttaca tcggctggca atactggaga tcccagggat tgtacgccag cagggaagca
     1201 cactgacact tgcaggcaac ggtgaagaac gctggaaatt aacccgcccg cttagccagc
     1261 atgcggcatt gattgaggcc gcctgctttg gtgccacact ccaggaagcc gcacgccata
     1321 aattagaagc cgatatgctg gacgcgggtg gaatcggcag tatcaccaca tgtcttagcc
     1381 aggcggcgtt agcgggtctg gcgtccttca gtcaacaatt actggagcaa ctcacattat
     1441 taatcgccca ggaaaatcaa tttgccgaaa tgggccaggc gctggaagtg ctatatgcct
     1501 tatggcggct ggatgaaatt agcggtatgc aaggcgcgca gatattacaa acgacgttat
     1561 gcgcggctat cgatcgcacg ctgtggctgt gtgaatctaa cggcagacca gatgaaaagg
     1621 agtttcacgc tcacctgcat agctggcagg cgctttgcca tatcctgcgc gatctacata
     1681 gcggcgttaa tttacccggc gtttttcttt ctgcggcggt agccttactg gagcgccgca
     1741 gtcaggcgat tcatgtcccg gcgctggatc gcggcgcggc tcttggcgca ctgatgcgtc
     1801 tggaacatcc caacgccagt gccgaagcgg cgctgacgat gctggcgcag ttatccccgg
     1861 cacaatccgg cgaggcgctg cacggtttgc tggcgctcgc ccgtcatcaa ctggcctgcc
     1921 aaccagcatt tatcgccggt ttcagtagtc atttaaatca actgagtgac gacgatttta
     1981 ttaacgccct gcccgatttg cgcgcggcga tggcctggct accgccacga gaacgcggga
     2041 cactggcgca tcaggtgctt gagcattacc agcttgcgca gcttcctgtt tcagcgctgc
     2101 aaatgccgtt gcattgtcct ccgcaagcca ttgcacatca tcaacaactc gaacagcagg
     2161 cactggcatc gctgcaaaac tggggagttt tccatgtctg aactgaacga tcttctgacc
     2221 acccgtgagc tacaacgctg gcgattaatt cttggcgaag cggcagaaac gacgctttgt
     2281 gggctggatg acaacgcccg gcagatagac cacgcgctgg aatggctgta tgggcgcgat
     2341 cctgaacggc tccagcgtgg tgaacgctcc ggtggattag gtggctcaaa tctcaccact
     2401 cctgagtgga tcaacagtat tcacacgctg tttccgcaac aggtgattga gcggctggaa
     2461 agcgatgccg tactgcgcta cggcattgaa gatgtggtga cgaatctcga catgctggaa
     2521 cgtatgcagc cttctgaaag cctgctacgc gccgttttgc acaccaaaca tctgatgaat
     2581 cccgaagtac tggctgccgc ccgccggata gtgcgccagg ttgttgaaga aattatggct
     2641 cgactggcaa aggaagttcg tcaggctttt tctggtgtcc gcgatcgccg tcgccgctca
     2701 tttattccac tggcgcgaaa ctttgatttc aaaagtactc tgcgcgctaa cctgcaacac
     2761 tggcacccgc aacacggcaa gttgtatatc gaatcccccc gctttaacag ccgcattaaa
     2821 cgccaaagcg aacaatggca actggtctta ctggttgatc aaagcggatc gatggtggac
     2881 tcggtgatcc actctgcggt gatggcggcc tgtttgtggc agttacccgg cattcgtacc
     2941 catctggtgg cgtttgacac aagcgtggtt gatctcacgg cagacgttgc cgatccggta
     3001 gagttattaa tgaaagtaca gttgggcggc gggaccaata tcgccagtgc cgtggagtat
     3061 ggtcggcaac ttattgaaca accagcaaaa agcgtcatta tcctcgtgag cgatttttac
     3121 gaagggggtt catcatcatt gctgacgcat caggtgaaaa agtgtgtcca gagcggcatc
     3181 aaagtgctgg gactggctgc gctcgatagc accgcaacac cttgctatga ccgcgatacg
     3241 gcccaggcgc tggttaatgt cggcgcacaa atagccgcca tgacgccggg cgagctggca
     3301 tcatggcttg cggagaatct tcagtcatga attcactacg tccggaatta ttagaactga
     3361 caccgcaggc cctgacggcg ttaagcaatg ccggttttgt taagcgcagt cttaaggaac
     3421 tggaaaatgg caacgtcccg gagatcagcc atgagaacga cgctttaatc gccaccttca
     3481 gtgacggtgt ccgtacccag ctggcgaacg gccaggcact gaaagaggct cagtgcagtt
     3541 gcggggccaa cggtatgtgc cgtcatcgcg tgatgctggt gttaagttat caacgacttt
     3601 gtgccaccac tcagtctacg gaaaaagaag aagagtggga tccggcaatc tggctggaag
     3661 aactggctac ccttcccgat gctacccgca aacgcgcaca ggcgctggtc gctaaaggca
     3721 tcaccattga gttgttctgt gcgccgggtg aaattccctc tgcccgctta ccgatgagcg
     3781 atatgcgttt ttattcccgc agcagtattc gtttcgcccg ttgtgattgt attgaaagca
     3841 cactttgcga acatgtcgta ctggcagtac aggcctttgt ccaggcaaaa gcgcagcaag
     3901 cggaattgac tcatttaatc tggcagatgc gcagcgaaca cgtcacatca tctgacgatc
     3961 cgtttgccag cgaagaaggc aaaacgtgtc gtcaatatgt tcaacaacta agccaggcat
     4021 tatggctgag cggcatcagc cagccgctta ttcactacga ggccgctttc agccgcgcgc
     4081 agcaagcagc ggaacgctgc agctggcgat gggtgagtga atcgctacgg caactgcgcg
     4141 ccagcgttga tgccttccac gcccgcgcca gccactatca tgccggagaa tgcttacgtc
     4201 agcttgcggc attaaacagt cgattaaatt gcgcacaaga gatggcccgg cgcgacagta
     4261 ttggtgaagt tcctcctgtg ccgtggcgca cggtcgttgg ctctggcatt gccggagaag
     4321 caaagcttga tcatctgcgg ctggtgtctt taggtatgcg ttgctggcag gatattgagc
     4381 attatggttt acgcatctgg tttaccgatc ccgacaccgg cagtattttg cacctttcgc
     4441 gcagttggcc gcgaagtgaa caggaaaact caccggcagc tacgcgtcgg ctgtttagtt
     4501 ttcaggctgg cgcactagcg ggcgggcaaa ttgtttcaca agcagcaaaa cgcagtgccg
     4561 atggcgagct gctgttagct acccgcaacc gcttaagcag cgttgtgccg ctgtcgcctg
     4621 atgcctggca aatgttgagc gcgccgttac gccagccggg cattgtggct ttgcgggaat
     4681 atttacgcca gcgtcccccc gcctgcatac ggcctcttaa tcaggtcgat aacttattta
     4741 ttctgccggt cgctgagtgt atttcgctcg gttgggatag cagccgccag acgctggatg
     4801 cgcaggtaat cagcggcgaa ggtgaagata atctcctgac gttatccctt ccggcatctg
     4861 cctgttctcc ttttgccgtt gaacgcatgg cggcgctttt gcaacaaaca gacgaccccg
     4921 tgagtctggt ttctggcttt gtcagctttg tagaggggca attgacactg gaaccacggg
     4981 tgatgatgac aaaaacccgc gcctgggcgc tggacgcaga aactgcgcct gtggcaccgc
     5041 taccttctgc cagcgttttg cctgtgccgt ctaccgctca tcagttgctg atgcgctgcc
     5101 aggcgttact tattcaactg ctccataacg gctggcgcta tcaggaacag agcgctatta
     5161 gtcaggcaga gttgctggcg aatgacctct ccgcggtcgg tttttatcgg ctggcacatg
     5221 tgttgggaca atttcgtaat acagaaagcg aggcacgggt agaagcaatg aataacggtg
     5281 ttttactttg cgaacaatta ttccccatgc ttcagcaaca aggatgaaat agtgcttact
     5341 aagagttcta ctccagttcc ggactgctca cgccacggta ttaggcatat cctatatagc
     5401 ccctggtgag agtcaccagt tccttgatta aataaaatgg agttttacat gaaggctttc
     5461 aataagctgt tttccctcgt tgttgcatct gttctggttt tctctcttgc tggctgcggt
     5521 gacaaagaag aatcgaagaa attcagcgcc aatctgaacg gcactgaaat tgccattacc
     5581 tatgtctaca aaggtgacaa ggtgcttaag caatcttctg aaaccaaaat tcaatttgcc
     5641 tccattggtg caaccaccaa agaagatgct gccaagacac ttgagccgtt aagcgccaaa
     5701 tacaaaaaca tcgcgggtgt tgaagaaaaa ttaacctata ccgataccta cgcgcaggaa
     5761 aacgtgacta tcgatatgga aaaagtggat tttaaagccc tgcagggtat ttcaggaatc
     5821 aacgtttctg ctgaagatgc caaaaaaggt atcactatgg cgcaaatgga actggtgatg
     5881 aaagccgctg gttttaaaga agtgaaataa tctgtcggcg gccatgtttc gcatggccgc
     5941 catacccgtc ttagcttttc ttcacatgct ggcgcgctgc cagtccacgc agaaaataac
     6001 gtaaaaattg atcgccgcat tcgcggaagt ttttatgatc cggtgcgcgc atcatcgctg
     6061 taatttccgg catcgaaacg cggaactgct gttcggtgag gatcgccaga atgtcatcgg
     6121 ttttcagcga aaacgcgatg cgtaattttt tcagcacgat gttgttatta atgcgacgtt
     6181 ccggctccag tgccggagca gactcatcct tgccgcgttt ttcataaatc aggccattga
     6241 ggaatgacga caaaacaatg tccggacaac gctgaaaacc ctcttcgtct tctttacgta
     6301 gccagacggc gatctgttcc gcggtggctt cgacattacc cagcgccaga atacgcacca
     6361 ggtcattatt attggctttc aaaatgtagc gcacgctgcg cagaatatcg ttacttagca
     6421 tgaggccttc aggtgttaat gaggcaaaaa gccattttag cagtctttta caggccaatc
     6481 gcctctttta agcttttcag ataacggcgg ctgaccggca cggttaagcc attacgcaaa
     6541 atcaactcgg cctggccgtt atcttccaga cgaatctcct gtaaatgcgc gaggttaacc
     6601 agatactgac gatggcagcg cagtagtggt gtacgacttt ccagggtacg taatgtcaat
     6661 tcggtaaagc cctctttccc ttcgtggctg gtaacgtaga caccgctcat ccgactgctg
     6721 acaaatgcca catctttcat ttgcagcaaa taaatccgac tatgccccgt acaagggata
     6781 aatttcagcg cctgttgatt ttccggtaac agcgaaacat cctgcttgct gcgctcctga
     6841 cgcaatcgcg ccagcgtttt ctccagtcgc gcttcatcaa ttggcttcag cagataatca
     6901 aaggcatgtt cttcaaaggc tttaatagcg tattcgtcaa acgcagtgag aaaaacaata
     6961 tacgggcgat gttccgggtc aagcatcccc accatttcca gaccactgat gcgcggcatc
     7021 tggatatcga gaaacagcac atccgggcgc agtttatgca ccgcgccgat cccttccacg
     7081 gcgtttgaac actctccaac gatttcaata tcgctctgct cctgcaaaaa tacacgcagg
     7141 ttctcccgtg ctaacggttc atcatcgaca attaagactt taatcatgcc tcgtccctcc
     7201 atggtagtcg taacgttatt cgggtgtaac tatcaggctc acaggcgacg cttattccat
     7261 agtcatcgcc aaaccgttca cgtaaacgct tatccaccag attcatcccc agcccactgg
     7321 cattggttac cggttgatac aaaccggcat tgtcttcgat ctccagcatc aaatgttgcc
     7381 cctcacgtcg ggcgctgatt gccactcgcc ctgtatccag cagttgtgat gtcccatgtt
     7441 taatggcgtt ttccactatc ggttgcaggg taaacgcggg caattgctgc tgggataatt
     7501 cttgcggaat agcaatgttg acctgcaacc gcgactggaa gcgcgccttt tcaatttgca
     7561 gataagcgtt cacatgttca atttcgtcgg cgagagtaac aaactccgaa ggccgcttta
     7621 agtttttgcg gaaaaaagtg gaaagatact gcaccagctg gctggcctgt tcgctgtcgc
     7681 ggcggatcac cgctttaatg gtgttaagcg cattaaacaa aaaatgagga ttcacctggg
     7741 cgtgaagcag tttgatttct gactgggtga gcatcgcttt ttgccgctca tattgcccgg
     7801 caagaatctg cgccgaaagc agttgcgcaa tcccctcgcc cagcgtgcgg tttattgaac
     7861 tgaataaacg gtttttggct tcatacaatt tgatggtgcc catcacgcgc tgattttcgc
     7921 cacgcaacgg aattaccagc gtcgacccca gtttgcattg cggatgcaaa gagcaacgat
     7981 aaggtacttc gttgccatca gcgtagacca cttcaccggt ttcaatcgct tttaaggtgt
     8041 aaggcgaaga aatcggtttg ccgggtaaat ggtggtcgtc accaattccg gtaaaggcca
     8101 gcaatttctc tcgatcggta atcgcgactg caccaatatc cagctcctga tacagcacct
     8161 gagccacttt catgctgttc acttcgttaa acccctgtcg caaaatgcct tccgtcgagg
     8221 ctgccacttt cagcgcagtg gcagaaaaag ccgaagtgta tttttcaaac atcgcgcgtt
     8281 tatcgagcaa tatacgcata aacagcgccg cgccgacggt attggtgacc atcattggcg
     8341 cagcaatatt actcaccaga cgcaccgcat cttcataagg tcgggcgatc gccaggatga
     8401 tcagcatttg caccatttca gcgacgaacg tgacggcacc ggcggtaatg gggttaaaga
     8461 ctttatcagt gcgcccgcga cggatcagga tgctgtgtac caggccgccg agcaatcctt
     8521 caacgatggt tgagatcatg caacttagcg cggtcatgcc ccccatcgaa tatcgatgta
     8581 agccgccggt cagaccaacc agcccaccga cgaccggacc gccgagtaag ccgcccatta
     8641 ccgcgcctat cgcacgggta ttggcaatag aatcgtcaat gtgcaaccca aaccaggtgc
     8701 ccatgatgca gaagatggaa aagacgatgt agcagagaaa tttatgcggc agacgaaccg
     8761 tgacctgcat taacggtatg aataatggcg ttttactcat taaccacgca atgactaaaa
     8821 aaacgcacat ctgctgaagc agcagcaaca ccagattaaa atcgtacata cccgcaaacc
     8881 acacttccct ttaaaacgcg taacatacat tgcctgcgtt taactttctt tgaa
//
LOCUS       DAJVRF010000152         8891 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJVRF010000152 DAJVRF010000000
VERSION     DAJVRF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8891
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.152"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            406..1281
                     /locus_tag="OQN13_003402"
     CDS             406..1281
                     /locus_tag="OQN13_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HCS1616676.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1289..2065
                     /locus_tag="OQN13_003403"
     CDS             1289..2065
                     /locus_tag="OQN13_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HCS1616677.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2234..4495
                     /locus_tag="OQN13_003404"
     CDS             2234..4495
                     /locus_tag="OQN13_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCS1616678.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            4564..5739
                     /gene="senB"
                     /locus_tag="OQN13_003405"
     CDS             4564..5739
                     /gene="senB"
                     /locus_tag="OQN13_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HCS1616679.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(6265..6726)
                     /locus_tag="OQN13_003406"
                     /pseudo
     CDS             complement(6265..6726)
                     /locus_tag="OQN13_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(6764..8302)
                     /locus_tag="OQN13_003407"
     CDS             complement(6764..8302)
                     /locus_tag="OQN13_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCS1616680.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPMEEALHGYVLTDGKLHVDDTPVPVLLPGNKKTKTGRLWAYVRDDRNA
                     GSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHA
                     RRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKN
                     YLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVA
                     LPTE"
     gene            complement(8352..8699)
                     /gene="tnpB"
                     /locus_tag="OQN13_003408"
     CDS             complement(8352..8699)
                     /gene="tnpB"
                     /locus_tag="OQN13_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1616681.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT         2322 a         2204 c         2158 g         2207 t
ORIGIN      
        1 tttcgcacag tatgtggctg atacgcacac attaacgtac gttttcgttc cattggccct
       61 caccccccga taacgaccca ggtagcgcgc cgtattcccc ccgccggccg ttttcttcga
      121 catgtacatc aggcgcggaa ctcatcgggg cgcgggaatg aaatcccatt cagactccgg
      181 atagattcaa gcaagcccac ttatcgtcaa aatcggtgtt gcaaaaacgg gagtgaccat
      241 agatccgttt tccaagcgga ccccataata tcggctggta aacatcactc gctagtgcat
      301 tctttttgtt ggtaatgata atcatttaca ttcattcttt gatgttttat cataattaat
      361 gttttgtcaa taaatatatg gaaacatatt gtttcagatg ggaatatgag aaagttcatc
      421 cttatttcta tgataacgtt agctagtgtt agtctgggag cctgcagaca gaatgtcacc
      481 ataaagcagg atgcgccagg gcagaaagct ttcctgactg acgggcagat ttacgatttg
      541 cacagtggca aaattatttc tagcagcgag cttttggcgg atcttgctac cgctcgacat
      601 cttattattg gtgaaaaaca cgataatgcc gagcatcatc agattgagct ttggcttatt
      661 caaaatttgc tcatccagcg accacagggg agtgtgttgc ttgagatgtt gacgtcagag
      721 caacagcccc gagttaacca agttaaatgt tggctgaaag ataatcctgt ggttcgagat
      781 agccgtgttc aggaactact taactggcaa aaagggtggt cctgggagat gtatggcgac
      841 atcgtcatgc agctattacg tgggccttac ccgctgctca atgccaacat tggccgggag
      901 cagatgttgg cactgtacaa aaaaaacgaa tttcctaaag gaaaaaaatc cactgcacct
      961 gtggtacaag aagctctgcg tgaaaccatc atatctatgc atgaaggcaa ccttgaaagc
     1021 cagcaactga cgtcaatgct ctctattcag cagcagcggg atcgctacat ggcccgccag
     1081 cttctgagtg ccccggtacc gtcgttgttg attgcaggtg gttatcacgc gtcaaaaagc
     1141 atgggcgtac cgttacacat ggaagatctt gccaccggca cccatccagt ggtgttgatg
     1201 cttgcagaaa aaggaatgaa catcacagta gatcatgctg actacgtgtg gtttgtagcg
     1261 ccagatacaa caaaaaggta aaccatggat gatgaatatt ctccacttct cacagtcggt
     1321 aaaatggagc agctggttta tctgttctct actgcttcat ggactgattt tccttgcgtt
     1381 tatctggcgt ttttccgaag ttcagcccgc tatgtctcct gcaccggcga tcatgctgca
     1441 atgggcagaa attatagagg ctccttcttc tccactttcg cttccggtgg gaatcgctca
     1501 acaggagtcc gcagtgacag aagaaaagca acaaactgag gacagacaac aacgtcctgt
     1561 cactgaagac agtgatgcaa ccattgaaat aactaggaag aaaaagtcat ccgatggaga
     1621 aaagaaaaag acacgtccac ctcgtaagat aaaggctcaa accagcgaca gcaaccccac
     1681 agccgtttcc agtaatgctg cacctcaggc tctggtcgaa tcgtcccgta ttgctgcgcc
     1741 ttttaacagt gactcaacaa agcgagataa ttctgaggca tcttgggaaa gcagagtaaa
     1801 aggccaccta aaccgttata aacgttaccc tggcgatgcc agaaaacgtg cacgaaccgg
     1861 cacagccgtc gtgactttta ccgtaaacac ggaaggcact attgtcagta gtttcttgga
     1921 aatctcttca ggaacccttt ctcttgaccg cgaagctatt gctgttttag aacgcgctca
     1981 accacttccc aaaccaccgc ctgaaatatt ggagggagga ttatttaaag taaaaatgcc
     2041 gattaccttt aagttgaagg agtaaaggga taattggccg ttcaagttca tttaactgaa
     2101 atttaatttt catttttatg ctgaacattt tatcaaactt ttccactgtc gaaaaaatca
     2161 aaaagacagt agaacataat taataaacaa atagttatac ttatctttat taatattata
     2221 cccaaggaaa tttatgaacg ttataaaact ggctatcggc tcaggcatat tattgctcag
     2281 ctgcggtgct tactcacaat ccatcagtga aaaaactaat tccgacaaaa aaggagcggc
     2341 agaattcagt ccgctcagcg tttctgtcgg gaagacgacc agtgagcagg aagctctcga
     2401 gaaaacaggc gcgaccagtt cccggacaac ggacaaaaac ctgcaatcac ttgacgcaac
     2461 agtgcgtagt atgcctggta cttatactca aatagatcct ggtcagggag caatcagtgt
     2521 gaatattcga ggcatgagcg gatttggtcg tgtaaacact atggtcgatg gtattaccca
     2581 gagtttttac ggaacctcta cctccggaac aacgacgcat ggttcaacta acaatatggc
     2641 tggcgtactt atagatccta acttactggt agcagttgat gttacacgcg gtgacagcag
     2701 tggctctgaa gggatcaacg cccttgccgg tagtgcaaat atgcgtacta ttggcgttga
     2761 cgatgtaata tttaacggta atacatatgg ccttcgttca cgtttctctg tcggtagtaa
     2821 tgggctggga cgcagcggaa tgatcgccct tggtggaaaa agcgacgctt ttacggatac
     2881 gggaagcatt ggcgttatgg ctgctgtgag cggcagttct gtgtactcta atttctcaaa
     2941 tggttctgga attaacagca aagagtttgg ttatgataaa tatatgaagc agaaccccaa
     3001 atcccaactg tataaaatgg atatcagacc agacgaattt aacagcttcg aactttccgc
     3061 tcgaacctat gaaaataaat ttacacgtcg tgatataacc agtgacgact attacattaa
     3121 atatcattac accccttttt ctgaattaat tgactttaac gtaacggcca gtaccagtcg
     3181 cggtaatcaa aagtatcgtg atggctcgct gtatactttc tacaaaacct cagcgcaaaa
     3241 tcgttctgac gcgctggata tcaacaatac cagccggttc actgtcgcgg acaatgacct
     3301 ggagtttatg ctgggcagca aactgatgcg tacccgctat gaccggacca ttcactcagc
     3361 ggcgggcgac ccgaaagcga atcaggaatc gatcgagaac aatccgttcg caccctccgg
     3421 ccagcaggat atttcagcgc tgtataccgg gctgaaggtt acgcgcggca tctgggaggc
     3481 agatttcaat ctcaactaca cacgtaacag gatcacaggg tacaagcccg cctgcgattc
     3541 acgcgttatc tgcgtgccac agggtagcta cgatattgac gataaagagg gtggcttcaa
     3601 cccttcagtt cagctttctg ctcaggtaac accatggctt cagccgttca ttggctacag
     3661 caaatccatg cgcgccccga acatccagga gatgttcttc tctaattcag gaggcgcatc
     3721 catgaaccca ttcctgaagc ctgaacgtgc agaaacctgg caggcgggtt ttaacattga
     3781 taccagagat ttactggtcg aacaggatgc cctgcgcttt aaggctctgg cgtaccgcag
     3841 caggatccag aactacatct acagcgagtc ttatctggtt tgttctggag gtcgtaaatg
     3901 cagtatgcct gaggtgattg gcaatgactg ggagggcatt agcgatgaat acagcgacaa
     3961 tatgtacatc tacgttaact cggcaagcga cgttatcgca aagggcttcg aactggagat
     4021 ggattatgat gcaggttttg cttttggccg actctctttc agccagcagc aaacagacca
     4081 gccaacctcc atcgccagca cctactttgg cgcaggggat atgaccgaac tgcccagaaa
     4141 atacatgacg ctggatactg gtgttcgctt cttcgataac gcgttgaccc tgggcactat
     4201 cataaaatac acaggcaagg ctcgtcgcct gtcgcctgat tttgagcagg acgaacatac
     4261 cggcgcaata atcaaacagg atttgccgca gatcccaacg attatcgatc tctatggtac
     4321 ttacgagtac aaccgcaacc taacactgaa actttcggta caaaacctga tgaacagaga
     4381 ttattcggag gcgctgaata agctcaacat gatgccaggt cttggtgacg agacccaccc
     4441 agccaattcc gcgcgtggca gaacatggat atttggcggg gacattcgtt tctgatgaca
     4501 atttccgcta ggttcagaca atacgttttc tctcttatgt caattttatt gcaggaacga
     4561 aaaatgaata ttttcacttt atccaaagca ccgctatacc tgttaatttc actattttta
     4621 cccacgatgg ccatggctat cgatccacct gaacgcgaac tttcgcgatt tgccctgaaa
     4681 acgaattacc ttcagtcccc tgatgaaggc gtctatgaac tggcgtttga taatgccagt
     4741 aaaaaggtgt ttgcagcagt caccgatcgt gtaaatcgtg aagccaataa aggctatctg
     4801 tattcgttta attcagattc gctgaaagtc gaaaataaat acacgatgcc ataccgggca
     4861 ttttcgctgg cgataaatca ggataaacat cagctctata tcggacacac ccagtcagcg
     4921 tccctgcgta tcagtatgtt tgacacccca accggcaaac tggtaagaac cagcgacagg
     4981 ttaagtttta aagcggcaaa cgctgcagat tcgcgttttg agcattttcg ccatatggtt
     5041 tacagccagg attccgatac cctgtttgtg agttatagca atatgctgaa aacggccgag
     5101 ggcatgaagc ctctgcataa gctgttaatg ctcgacggga cgacgcttgc cttaaaaggc
     5161 gaggttaagg atgcttacaa aggtacagcg tatggtctga cgatggatga aaaaacacag
     5221 aaaatctacg ttggcggaag agattacatc aacgaaattg atgcgaaaaa tcagacgctg
     5281 ctgcgtacca tcccgttgaa agatccgaga ccacaaatca caagtgtgca gaatctggcg
     5341 gtggactccg cttctgaccg tgcctttgtg gtggtattcg accatgacga tcgttccggt
     5401 acaaaagatg gactctatat ttttgactta cgcgacggta aacagcttgg ctatgtgcac
     5461 acaggagccg gagctaacgc ggtgaaatac aatccgaaat ataacgaact gtatgtcacc
     5521 aacttcacta gcggcaccat cagcgtagtg gatgccacca aatacagcat cacccgtgaa
     5581 tttaacatgc cggtctaccc aaaccagatg gtgttgtcgg acgatatgga taccctttac
     5641 attggcatca aagaaggctt taaccgcgat tgggatcctg atgtgtttgt ggaaggagct
     5701 aaagaacgta ttctgagcat tgatttgaaa aagtcgtgat cggggggaaa aaacatggga
     5761 taaaaactcc gttattccac taaactggcc ggactaggaa aagcaaacat ctctgtcctg
     5821 ccggtacgcc tgttacgccg ctccatgagg ctgaggaatc tccagaaaac gagacaggca
     5881 cagcattgta cgatcaactg atttgtgcca ataattaaat gaaatcacag ctatgcctgt
     5941 tcatggggtt ctaggccgga accccagatt tttccgattg tttgttttct aactgattat
     6001 tcagtagttt tagagggctg acgctcagtg gaacgaaaac tcacgttaag aggttttgac
     6061 tatgataaaa gtcaaagtag gggtgagttt ggatatggaa attattgtac tataaggaga
     6121 gcatgaaaac ccctggggca gtcactgaat gagggaccag cagcggtaaa ataaccagca
     6181 taatttctgc atggtcgatt tttataccgt gacaggctgt tttttatact tggggtcagt
     6241 ttggatatcg aaaattattg tacgttaagg ttgttttttg accttgtcga tcttaactga
     6301 tctcaaacga cgaaacttac ctgaagtcgg tatccggctg tttttccaga tataatcgcc
     6361 tgtcagattg atatgttccc aacctaatgg tgataaatgc gataatagtt gagcatttat
     6421 tttcatgccc ttacgcttca gagcatcaac cgctctttcc atgtaaactg tgttccataa
     6481 ggaaatagcc gccgtcaata aagtcaatcc actggcgcga taactttgat tttccggttt
     6541 ccgatctcta atctccccca atcgatgcat gaatacagca cgggccagag cattacgggc
     6601 ttctcctttg ttcagcccgg cctggacacg tcgccgcaat gccggatccc gaaaccagtc
     6661 cagcataaac agtgttcgtt ctatacggcc aatctctctc agagctttag ccaacccatt
     6721 ttgtttgtaa gcgtgcagca agaaccgtat tgacgggaat gtgttattca gtcggcagtg
     6781 ctacgcgcca ggggagcagt tcgccgaccc ggtttatcgg ccagtcggct atgacgtcaa
     6841 ggacatagcg gaggtagctt tctggctcca ctccgttcag tttgcacgtc ccgatcaggc
     6901 tgtacagcag cgctccccgc tctcctccat gatccgaacc gaagaacagg tagtttttgc
     6961 ggcccagact gaccatccgc aacgcatttt cagcgatgtt attgtccgcc tcagcccagc
     7021 catcatctgc atagtacgtc agcgccggcc actggttcag ggcgtatgcg aacgctttcg
     7081 ccagttctga gtgtcgcgac agggttttca tcttttcacg cagccagctt tccagggatt
     7141 tcagcagcgg tttcgttttc aactgacgtt cggcaaggcg ctgttccgcc gtcattcccc
     7201 ttatctctgc ctcgatggcg tacagttcgc cgatccgttt cagcgcttcc tccgtcaggg
     7261 ctgacggggt gcgaacgtgc acatcgtgga ttttacggcg ggcgtgagcc caacaggcgg
     7321 cttccgttat ccggccatcc cggtacagct cgttgaaccc ggcgtatgca tccgcctgca
     7381 gtacaccact gaaccccgca agatgggtct gcggatggat gccttttctg tccgggctgt
     7441 aagcgaacca caccgccggc gccagcgttg acccggcgtt acggtcgtca cgaacatacg
     7501 cccacaaccg cccggtcttc gttttcttat tgcctggcaa cagcaccggg acaggcgtgt
     7561 catcaacatg gagcttaccg tcagtcagca catagccatg aagcgcctct tccatcggag
     7621 acagcagccg gcagcatgca tccacccagc ccgacagcag tgaacggctc agctccacac
     7681 cttgccggcc gtatatttct gactggcgat acagcggggt gtgctctgca tacttcgagg
     7741 tcagcacgcg ggccagcagc cccggtccgg cgataccccg ctcgatgggc cgcgaaggtg
     7801 caggtgcctg cacgatggca tcgcactgag tacaggcatg tttttcccgt accgtccgga
     7861 taacccggaa ggcgctacgc atcaactcca gctgttcggc ggtatcctcg cccagatagc
     7921 tcagtgaacc gccgcagttc gggcagcacg gcgccgcagg caacagtcgc ttttcgtcac
     7981 ggggtagtga ttcagggaac ggcttacggg tgcgggtctg acgcaacgga cgctgtactg
     8041 ccgggtcata caccctacca gtcagcgtat cgctctcttt ctgaagccgg ttcagatcgg
     8101 cttccatttg tgcgatacgg cgggagactt tttcggaacg actgccgaag ttcatccggc
     8161 ggagtttatc cagctgcgcc tgcagatggt ctatttcgcg ctcccggttg ctaagctttt
     8221 cctgcagggc gtggatcagc gcttcctgtt cggccaggcg ctgtttcagc aggaagatgt
     8281 cgtcagaaga gatgtcgttc ataagcccgt attttaccgg gcttattctg tgacaaccag
     8341 gataaagaga tttacagcat ggtcagggag gtcagcagcc gcttaggctg tcgccagtcg
     8401 ataccttcca gcagcatcgc cagctgcgcc tgtgtaagga acactttgcc atcacgggct
     8461 gacggccagg cgaagcgccc acgctccagc cgtttggtca ggaggcacag tccgtcactg
     8521 gtggaccaca gcagtttaac ctgactgccg ctgcggcccc ggaaaatgaa aacatggccg
     8581 gacatgggat cgtctttcag cgccgtctgt actttcgcag ccaggccgtt gaagccattt
     8641 ctcatatcgg tgataccggc aaccagccaa attttgatcc cggaaggtaa cgggatcatc
     8701 gcttcagttc ctgtatcagc agagtcagga gcttttcgct gacattgcca ttgaagcgga
     8761 gcgtcccgtg ccggaacgtt acctcacagc tgatactgag ggttttcggg tcctctgcga
     8821 gcgattctgg ctgttcggca gctgcatcga gagtcacagg aagtagctgg gggctctctg
     8881 aagaaggtaa t
//
LOCUS       DAJVRF010000153         8840 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJVRF010000153 DAJVRF010000000
VERSION     DAJVRF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.153"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            814..1056
                     /locus_tag="OQN13_003409"
     CDS             814..1056
                     /locus_tag="OQN13_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616682.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1386..2315)
                     /gene="fdhE"
                     /locus_tag="OQN13_003410"
     CDS             complement(1386..2315)
                     /gene="fdhE"
                     /locus_tag="OQN13_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCS1616683.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2312..2947)
                     /gene="fdoI"
                     /locus_tag="OQN13_003411"
     CDS             complement(2312..2947)
                     /gene="fdoI"
                     /locus_tag="OQN13_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS1616684.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2944..3846)
                     /gene="fdoH"
                     /locus_tag="OQN13_003412"
     CDS             complement(2944..3846)
                     /gene="fdoH"
                     /locus_tag="OQN13_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCS1616685.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3859..6909)
                     /gene="fdnG"
                     /locus_tag="OQN13_003413"
     CDS             complement(3859..6909)
                     /gene="fdnG"
                     /locus_tag="OQN13_003413"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6322..6324),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS1616686.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7103..7936
                     /gene="fdhD"
                     /locus_tag="OQN13_003414"
     CDS             7103..7936
                     /gene="fdhD"
                     /locus_tag="OQN13_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCS1616687.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            8089..>8840
                     /locus_tag="OQN13_003415"
     CDS             8089..>8840
                     /locus_tag="OQN13_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HCS1616688.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFK
                     QGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQE"
BASE COUNT         2121 a         2233 c         2265 g         2221 t
ORIGIN      
        1 tcatttacat cattccctgt ctatgccggt aatattgaat aagaactcta taaattttta
       61 ttcttcgttg gcaccatacg tgtcttgcta catcactgtt caggagcatt taaatattat
      121 ttgtagagct gagtggcaat tatagcagat aatgcttaac tcatcatctt agttcaggtt
      181 tgagcgattt ttgatagtca aaacctcctc tggtgccata cttttctcat aaatattttt
      241 aaaatctata gctcacaaac attatgcaaa gataatgggc tggcatcatt gtcagtatca
      301 ttcatttact gctctgtatg ttatgaatca ttatgttgta tgtttgctct taaaccgtgg
      361 gaaggaggct ataatggttg ccaatattaa tctcattaaa caagaaagtt attctgttgt
      421 taatttagaa aaacaacttt cccgaactgt taccaacaaa attataacga tgagcaagta
      481 aagaccggtt ggtggcagta ctgaccggtc tgtgatgaaa gattaatttc accttaaatc
      541 ctggtgctgt taggatgctg acgtttttcg gctacaccat tcagattatg caaataaata
      601 tccctgaata taatatattc atagcaccca taactcatcc tgggcgataa ctaaaccatt
      661 tttgagtaat aaatgaaaca actgcaacgc tcttattatt tctggaaaac gcctcgcaaa
      721 aataaaaaat gatgcgtaaa tgagctttct ttaaagattc ggcaagggta cttttctctc
      781 catagattca aaaatgatat tggcgagata tttatgaact ctctggcagg tatagatatg
      841 ggcagaattt tactcgattt atcgaatgag gtgattaagc aacttgatga tctcgaggtg
      901 cagcgtaatc ttcctcgtgc ggacctatta agggaagcag tagatcaata cctgataaat
      961 caatcgcaaa cagcaagaac cagtgttcct ggcatctggc aagggtgtga ggaagatggt
     1021 gtcgaatatc agcgtaagct gcgcgaggaa tggtaatggg aaaaaatgat gtcaatcaga
     1081 ttgctgacaa cgtgcgcgtt gttcatgccg gatgcggcgt gaacgcctta tccggcctac
     1141 aaagtcatgc aaattcaata tattgaagtt tattggtagg cctgataagc gcagcgcatc
     1201 aggcaatcat acgcttgtca tcagtgacat acccataacc ttatcgatgt gttggaggga
     1261 agaacgaggc aaacagacaa ttgcggcatg atcttgaatg ggtaacgcgg aatacgagag
     1321 atggtgtcgt cacaccctat gagctgtaat ccgggcgacc tgcgccaccc ggaaaacgtc
     1381 agagattact ctccttcacc cggaaacagg aacgggttaa tggaactacg ggcgtagcct
     1441 tcttgctcca ttcgcgcgtc cagtaccaga gaggcgaggt catctgccac ggcttcaact
     1501 ttcggttctt tttcctgata aagaatcttc aggtaagtgc cgcagtcatc gcagctttcg
     1561 gctttaatcg cggcctgttc gtcatccagc gaccagtaat gcagtttgcc gctctgctcg
     1621 cagttgctgc attttacgcg cactacgtgc cattcggttt cacacaggtt gcagtgcagg
     1681 taacgcagac cctgagtggt gccaatttgc accatgctgg acaccggcat agagccacat
     1741 accgggcaat attgacgttg ttcgccgtat tcagcgcggg ctttgccggg gatcagattg
     1801 gccatctgcg cccagtagag cgacagcgca gcccagataa acggcgcttt atcgctgctg
     1861 acggacgaga aatcagaggc aaacagcgcg ctggccatat cttccagctc ctgagtcgat
     1921 gccttctcca gattctcaat cactgccagc gccgggccgc tcatttcagg tttcagctca
     1981 gcaattagcg ccatcagcag cttttgccag tgcttatcac gcggcagaac gtgaatatcc
     2041 agcgggggct tgccctgtgc gctggcttct ttaatgcgcg cggtcagatc catctccagc
     2101 ggatggtcgt acagcaccac ttcctgggcg tgggcgataa gcgcagcaaa gcgcaggtaa
     2161 tcacccagcg gattattttc tgccagctcg cgcagacgct cggcgcggcg gttgtataaa
     2221 ttcttgagcc gagggaacaa taacggcgga atcatatccg ccgtacgttt ctcgctcgaa
     2281 cccagctcat cttgcgggat tatgcgaata ctcattcagc tttcttttcc gttgtcttgc
     2341 ggacctcacg gtaccagcgc gggtgatgtt tcttcgccca ggcgctggta acccatcctt
     2401 ccaccatggc ggtaatcgtg cctttcaccc aaagggcggc gtagatatgc accatgataa
     2461 ccacaattaa cgccactgcg gcaaatgaat gcagcattaa cgcgaatcgg atcaccggga
     2521 ttgagaaagc aggcgcaaaa taaggacgcc agataatcac gccgctcacc agcagcagaa
     2581 ccaggaaaat aatcgccgcc cagaaaacgc atttctgacc gaagttataa cgcccggtgt
     2641 cacctacttc ctcgttgacg acgatcttac gaatattctt cgcccaaaag atatcatccc
     2701 gattgattag gttgtgatgc cagtaacgga aaaacatgat gatgaacgag gcaaacataa
     2761 ccacgccgac aaacgggtgc agaattcgcg ccagctgcgg tgtgcccatg atttgcatca
     2821 accagttgaa ggacgggaac aaaaagccca gcccgctcac cgccgccagg atgaagcaga
     2881 aggcggtgat ccagtggttg atacgttccg gcgcggtgta gcgcacgatg gtgtcacgtc
     2941 gtttcatttg cgctcctcgt ctttctcttc gtgcagatta ttctcttcct catccgcacg
     3001 gttcggaccg acacccacgt agtggaagat actggccgca aaggtagccg caaagccaac
     3061 agccgcgagc ggtttccaga tgcctttcca gaatttcacg gtttcgctga tttccgggtt
     3121 ctccggcaag ccatgataca gatttggctt gtcagcatgg tgcagcacgt acatgacgtg
     3181 tgtaccaccg acgcctgccg gatcgtacag acccgcattg tcgtaaccgc gggttttcag
     3241 ctcagcaacg cgctcgctcg ccagcgtttt catcgactct ttcgtaccga agtgaatcgc
     3301 gcccgttggg caggtcttca cgcaggctgg ttcttgccca accaccacgc ggtcaacgca
     3361 cagcgtacat ttgtagacgc ggttgtcttc cgggttgagg cgcggaatgt cgaacggaca
     3421 gcccgcaatg caataaccgc agccaatgca ctgctcggac tggaagtcga cgataccgtt
     3481 ggcatactga ataattgcac cttccgccgg gcacgctttc aggcagcctg gatcggaaca
     3541 gtgcatacag ccgtctttgc ggatcagcca ttccagtttg tcgttctgct ccacttccga
     3601 gaagcgcatc accgtccacg atttggcgct taaatcattg gggttgtcgt acaccccaat
     3661 gttattgccg acggtatcgc gaatgtcgtt ccactctgaa cacgccacct gacaggcttt
     3721 acagccgata caggtggtga cgtcgatgag tttcgccact tcttcctgga agtcccgcgc
     3781 ctgaggcgcg ggggtcagac cgttagtcgc ggaacgacga atgatatctt gcgattgata
     3841 agccatatgt cgtctccgtt acaccttttc cacattcaca aggaaggact taaactccgg
     3901 cgtctgcgtg ttcgcatcac caacgaatgg cgttaacgta ttggcaataa agcctttttt
     3961 agctacgcct tcatagcccc agtgaatcgg aataccgatg gtatcgatat ctttgccgtt
     4021 cgctttcagc gtgcgaatac gtttggtcac caccgctttg gctttgatat agccacggtt
     4081 ggaggagact ttcacggtat cgccctgggc aatgccaagt ttattcgcca gcgactcccc
     4141 gatttccaca aactgctctg gttgcaaaat cgcgttcaac agcgcgtgtt tggtccagta
     4201 gtggaagtgc tcggtcagac gataggtggt tccgacatac gggaacttat cggctttacc
     4261 caatgcttcg gcgtcgtctt taaagattcg cgcagccggg ttcgagataa cgtttggatg
     4321 cagcgggtta gttcccagcg gcgtttcaaa cggctcgtag tgttccggga acggaccttc
     4381 cgccatctta tcgagggcaa acagacgccc catgccttcc tgctgcatga taaacggccc
     4441 gacgccgctg cccggaggcg ctgcgctgta gtccggaata tcccagccgg tccacttagt
     4501 gccgtcccat ttcagcaact gacgcttcgg atcccacggg ttaccctgcg gatctgcgga
     4561 ggcgcggtta tacagaatgc ggcggttaag cggccatgcc catgcccagc ccagcgtgtt
     4621 accgaggcca gacggatcgg cgttatcacg acgcgccatc tggttgcctt ccggcgtcca
     4681 gctaccggcg aaaatccagc agccacagga ggttgtaccg tcatcgcgca attgggcgaa
     4741 cgagctaagt tgttggcctt tcttgacgat aaccgcaccg gttgccggat cggtaatatc
     4801 ggccagcgcc ttaccgttgc tctccatcgc cacttcttcc gacttcggct catgcggaat
     4861 ggcgtagttc caggtcatgt tcagcacctg atccgggttt gcgccaccct gttcggcata
     4921 catcttgcgc aagcgcagga agataccgga gaggatctcg ccatcagtca gcgcaatccc
     4981 tggggcgtcc gcacctttcc agtgccactg caaccagcga cctgagttaa cgattgaacc
     5041 gttctcttct gcgaagcagg tcgatggcag acggaacact tcggtctgga tcttcgacga
     5101 gtcaacttcg ttcagctcac cgtggttctg ccagaagtta gaggtttcag tgttcagcgg
     5161 gtcgatggtg acgaggaact tcagtttcga cagacaaccg atcactttgt ttttgttcgg
     5221 gaatgaggca acagggttaa agccctggca gatatagcca ttgaccttgc cctctttcat
     5281 catctcgaag tattgcagga cgtcgtagcc tttatcccac ttcggcaacc agtcaaagcc
     5341 ccagctattt tccgccgtcg ctttatcacc aaagaaggcc ttcatcatag agacgaagaa
     5401 tttcgggtag ttgccccagt agtttacctg gccttccagc agcggttttg gcgtgttggc
     5461 ggtaaggtag gtttgcagat cggtctgctt ctcgcttggc agcgtcatgt aacctggcag
     5521 gctctgcgac agcagcccca ggtccgtcag cccctgaata ttggagtgac cgcgcagggc
     5581 gttaacgccg ccgcctgcca tccccatatt gccgagcagc agctggatca tcgccatcgt
     5641 acgaatgttt tgcgcaccaa cggagtgttg cgtccagccg agggcataca ggaacgaggc
     5701 agttttatcg tgagcactgg tttctgcgat gtattcgcag actttcagga acgcgtcttt
     5761 tggcgtacca cagatgtttt caaccacatc tggcgtgtag cgggaaacgt gctgtttcag
     5821 caagttccac acgcagcgcg gatgttgcag cgtggtatcg cgtttggcga agccgttttc
     5881 gtccagttcg taagtccagg aggatttatc gtacttgcgt ttttccgcgt catagccggt
     5941 gaacaggcca tcttcaaagc cgtaatcctc acgcacgatc aggctggcgt tggtataggc
     6001 ttcggtgtat tcgcggttga atttttcatt gttcagcagg tacagcaata cgcctgacag
     6061 gaaagcaatg tcagtaccgg aacgaatagg ggcatagtag tcagccaccg ccgccgtacg
     6121 cgtaaagcga ggatcgatca caatcagctt cgcgccattg tgaattttgg cttccatcgc
     6181 ccagcggaac ccgaccgggt gagcttcagc ggcgttaccg cccatcacca cgacgaggtt
     6241 ggcgttcttg atgtcgaccc agtggttggt catcgcaccg cgaccaaatg ttggagcaag
     6301 acttgctacc gttggtccgt gtcagacacg cgcctggttg tcgaccgcga gcatacccag
     6361 cgcgcgggag aatttttgcg ttaaatagcc ggtttcgtta ctcgacgcgg aagcacacag
     6421 catcccggtg gagagccagc ggttaacagt cacgccttcg gcgttttgcg caatgtagtt
     6481 agcatcgcgg tcttctttca tcagtttggc gatgcgatca aacgcctctt cccaactgat
     6541 ttgctgccat ttatcagaac ctggcgcacg gtattccgga aacttcagac ggctttcgga
     6601 gtggatgaaa tccaccaggc cagcgccttt cggacaaagt gcaccgcggt tgaccgggtg
     6661 atccggatcg ccttcgatat ggaagataga tgctttggcg ttttttgctc cgtcaccgag
     6721 gctgtacatc aacagcccac agcctacgga acaataggtg caggtattac gggtttcgcg
     6781 ggtgcgcagc agtttatact gccgggtttc cgcgagtgct acgctgggtg caaaacccag
     6841 tgccgctgcc gtggtgcctg ccataccgcc agcgcagatc ttaaagaact gccttctgct
     6901 gacctgcatg gattgctcct tgtttcgaca ttgtcacgtc ccatttacat tcgcttgctg
     6961 cgtgtgcagg gagtgggagt tatttttctt tgcggaaggg gccgcaaagg tccagaattg
     7021 gctcaatttc cctccatcca ggaagggttt gtaacagaat accataatgt tggtgtgtgt
     7081 gttcttatct ggttaagaga aagtgaaaaa aacacagcga aaagaaatcg aaaatgtgac
     7141 aaatatcaca ggtgttcgtc aaattgagtt atggcggcgt gatgatttac aacacccacg
     7201 gttagatgag gtcgcggaag aagttcccgt tgcgctggtc tacaacggca tttcgcatgt
     7261 ggtgatgatg gcgtcgccca aagaccttga gtactttgcg ctcggttttt cgctttccga
     7321 ggggataata gaaagtccgc gcgatatctt cggcatggat gtcgttcctt cctgtaatgg
     7381 tcttgaagta caaattgagc tttccagccg ccgctttatg gggttgaagg agcgccgccg
     7441 ggcgctggcg ggacgtacgg gctgtggcgt atgcggcgtg gagcaactta atgacatcgg
     7501 aaaaccggtg cagccgctac cgttcaccca gacgtttgat ctcaacaaac tggatgatgc
     7561 attacgtcat ctcaacgatt ttcagccagt ggggcaactg actggttgta ctcacgccgc
     7621 tgcctggatg ttgccatctg gcgaactggt cggcgggcat gaagacgtgg gtcgccatgt
     7681 ggcgctggac aaactgttag gccgccggtc acaagaaggg gaaagctggc agcaaggtgc
     7741 ggtactggtt tccagtcgtg ccagttatga aatggtgcaa aagtcggcga tgtgcggcgt
     7801 agagattttg tttgcggtgt ctgccgcgac cacgcttgct gtagaagtgg ctgagcgctg
     7861 taatctgaca ctggtaggtt tttgtaaacc gggtcgggca acggtttata cccatccgca
     7921 gcgtttgagc aattaattaa aataagtcct gcgatatata ttaaattgca ggacttattc
     7981 atttcgtgaa ttttattatt ttatttataa ataaaacgcg tgtagtgacg ttttcttttt
     8041 tcatttatta tgttcactct cttatctata tatttataag gcaattaaat gaaaaggaat
     8101 ttattatctt ccgctattat agtcgccatc atggccctcg gtctgacggg ttgtgatgat
     8161 aaaaaagccg aaacagaaac gcccccgcct gccaatagtc aacctgccgc accagctcct
     8221 gaagctcccg ttgcaaaagc agaagctaaa cctgaaacac ctgcgcaacc ggtggtcgat
     8281 gaacaagcgg ttttcgacga aaaaatggat gtctatatca agtgctacaa caagttacag
     8341 atccctgtac agaacagtct ggcgcgttat gctgactggc tgaaagattt taaacagggg
     8401 cctaccggta aagagagtac tgtttatggc atctacggca ttagtgaatc caacctcgct
     8461 gagtgtgaaa aaggcgtaaa aagtgctgtg gcgttaacgc ctgcgctgca acctattgat
     8521 ggcgtggcgg tgagttatat tgatgctgcc gtggcgttgg gtaataccat taacgaaatg
     8581 gataaatatt acacccagga aaattataaa gacgatgcgt ttgcgaaagg gaaaacgctg
     8641 caccagacat tcttaaaaaa tctggaatcc tttgaacctg tagcagaatc ttatcatgcg
     8701 gcgattcagg aaattaatga taagcgccag cttgcggaac tgaaaaatat tgaagaaaga
     8761 gaagggaaaa cattccacta ctactctctg gcagtcatga tttcagcgaa acaaattaat
     8821 aacctgattt cgcaagagaa
//
LOCUS       DAJVRF010000154         8505 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJVRF010000154 DAJVRF010000000
VERSION     DAJVRF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8505
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.154"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(108..4519)
                     /locus_tag="OQN13_003416"
                     /pseudo
     CDS             complement(108..4519)
                     /locus_tag="OQN13_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR/RHS domain-containing protein"
     gene            complement(4539..5000)
                     /locus_tag="OQN13_003417"
     CDS             complement(4539..5000)
                     /locus_tag="OQN13_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HCS1616689.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5028..6929)
                     /locus_tag="OQN13_003418"
     CDS             complement(5028..6929)
                     /locus_tag="OQN13_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCS1616690.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDSIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            complement(7666..>8505)
                     /gene="cusS"
                     /locus_tag="OQN13_003419"
     CDS             complement(7666..>8505)
                     /gene="cusS"
                     /locus_tag="OQN13_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCS1616691.1"
                     /translation="LAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTEPIELEQLVLS
                     FNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNL
                     EELTRMAKMVSDMLFLAQADNNQLIPEKKMLNLADEVSKVFDFFEALAEDRGVELRFV
                     GDKCQVAGDPLMLRRALSNLLSNALRYTPTRETIVVRCQTVDHQVQVTVENPGTPIAP
                     EHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVIMLP
                     ERE"
BASE COUNT         1708 a         2498 c         2238 g         2061 t
ORIGIN      
        1 tttatttgac tacgtgataa acgaacccca tccaggaaac ccgtatgccc actaacacga
       61 cgaccatcgc caatatagtc catcaaactg ctgattttag aacttgcagc caatcccagc
      121 ggatcaaccc acccacaagg attcggcaca taccggtaag tctgctcccc gccctgcaac
      181 cccagcggat cactcaccag gtacattccg gtttccggct cgtaataccg gaaccgatta
      241 tagcacagcc cggattccgc atcctgccac tgtcctgcat acagcaggcc ggggtcggct
      301 tccgggtcca gttccccgcg cgggtccggg tagccggtgt ctgcgggcag gctgagtgcc
      361 agcccccaca ggctggtctg ccccggtcgc cagaccgtct ttccttcact gttaaagagc
      421 atcagcgggc ggcccgtcag gtcgctcacc gcatgattcg tccgcgtcac ccactgcggg
      481 gccaccgacg gatgcgcctg ccgtacccgg ctgaactgct ggcttatcag ctcaaagccg
      541 ttaaacacca ggtgccttac gctgtacagt ttatcatcgc ggtattcccg gatttccgca
      601 atactgtcgc cgtcccacag atacgtgaag cggattttct tccggtcgca gcgtttctct
      661 gttcgtctgc cgctggcgtc gtggcggtat tcccacagct caccctgtgc gctgcaatac
      721 ccggtcagct ggtcccggct gtcccaccgg aaccgctctg tttccggtcg gtagccgtca
      781 cggtgtctgg tgcggctgac catccggcct gcggcgtcat agtcatactg cgtgtttccg
      841 gcctgtttca gccggtggcc tccggcatac tggtcagtct cttcacttat ccggtgcctg
      901 cccgcagact gcgccttcag gtagccgcag ctgtcatagc cataggactc atcgccttcg
      961 ccatagccca gcccggtgcc tgaggctgtc accgccgtga cctggccgtt ccggtccagc
     1021 cgatactccc gccgtgtttc ccgtccgtaa tcttcccggc tggtgacgcc tgacacgtta
     1081 cccgcgctgt cgtaacggta ctcccgtacc agtgagggaa ccagcccgtc ccatgtggct
     1141 tctgccggaa aaaatccggc gcgctgactg acaggctgac cggctgcatt ccagccctgt
     1201 tcacaggcaa aaccccggtt actgtgccgg agcacttcac gtcccgcctc atcacggctg
     1261 aacgtcagcg tctcatcccc cggcagaatg acctctgtca gttctccggc ggcgttgtag
     1321 cggtacgtgg ttttcagcag ggattcaccg gggtgtctgt cgcgttcatc ttccggtggc
     1381 aggatacagc ggatgacggt gtgttcatcg gggtagttgt attcaatggc ccgtcggccc
     1441 tgagtggtga cattcacccg cccgcgttcg tcatagccgg tgcgctgtga tgtgccgtcc
     1501 gggctgaaga tacccgtcag cctgccggat ttgtcatagg catactgcgt tttgccttcc
     1561 ggcgagatga tttctgccag gcggcctgcg gcgtcccggc tgtagaggat ggtttcctcc
     1621 aggccgttaa ccttggcggt acagaggcca tcggcgtcat agcggtaacg ccagaccacg
     1681 ccgccccagt cacgctcttc cgtcacccgg ccgcagttat ccagaacata aagatatgac
     1741 tcaccctgcg ggttgatgac ttctgtcagt tgcagcgtgt ctttatcgta gcggtactgc
     1801 cagcggtggc cttcaccgtc cgttcgcgcc accggaaaat cgaacggacc gtattccatc
     1861 gtccagacaa caccgttgcc gtccgtccag gccagcaggt tgccgtggcg gtcatagcga
     1921 aatccttccg cgccggttcc agcacgttca atgccgctca gtttatggtg ctgctgccac
     1981 tgtaaccgca ctgactgacc gccggggccg cgcatcttca ctggcaggtc gtgaccgctg
     2041 tattcggtgt ggtgttcacg gcccatcacg tcggtgagtg tttccggccg gttgtggtgg
     2101 tcgtactcta tgcccagacg ccgttcatcc tggcggaata ttcccgacag cagcccctga
     2161 gcggtgtatg ccagccgggt ctcttcgccc tgtggcgaca ccagacttac cggcaggccg
     2221 cgttcatcac actgctgtgt ccacgtctgc ccggcagggt cggtgatgtt gctgagcagg
     2281 ccgcgctcat catagcggta ctgccactcc gcgccgtctg ccgccgtgta acggcttaac
     2341 tgaccgtgtg cggcgttgta ttcaaatttc tccacccgcc cggcagggga gtggcgggcc
     2401 agcaggtgat ggaattcatc ccattcatac atggtggtgc tgccgtccgg cgcttcttcc
     2461 cgcagaatat tgccgtcagg atcatagtaa taacggtgaa tgcccttgcc gtccgtcacg
     2521 gtggtgcagc cgtcaccata gtccagcgtg gcgttgtaat agccctccgc cccggtgaca
     2581 ttcacgcacc ggccctgttc atcgtaggtg aaacggctcc acgtctggtc gttatcggac
     2641 cagcggatga tccggtccgc agcgtcgtac tcataaaaca ggtggtagtc cagccgcgca
     2701 tccgcttctg tcagccgccc ccgcgcatcc tgttcgtagg tcgccagcgt ctgtatgtcg
     2761 ttatccgtgc ggtgaatggc tttcaggtag ccgctggcgt ggtacgtcag tttaagccgg
     2821 atcccgtcgg tgtgactgac acccgtgatc cggtactggc tgtcgcgctc aaacgtgatg
     2881 cggttgctgg cggtgtcgct catcccgccc agcatgtaca tccggtcatt ggggctgtgt
     2941 gccaggcggt catcgctggt atcgtcagtc accgggtggc gcagacgcat tcccggaaag
     3001 gcgtcataaa agaagcggct gctcagtgca tccctgtcga acagctccag ataatcatcg
     3061 cggcggcgca gaatgtactg ggcgtgatac gggttaaccg ccgtatcact gtcaccgtcg
     3121 aaggcaaatc tcagcgtggc tccctggctg atgatatcca cccggtttcc ctgttcacgc
     3181 acccaggcgt attcgctcca ggagtcgctc cagcccacgc cggtcaggcc atgcagcggc
     3241 agtacagaac tgtggaaacg ggtgaaggag agggggaggg tctggcccag ggtgaagtcg
     3301 gtacgttcgt cgcagtatgc gccggttacc ggatcgacag gatctgttac ccatctgatg
     3361 actttattgg caatactttt taacgactta cctgttttcc ccagcaacgc gccgagcttt
     3421 gcgcccagca gcttcccgct tttccccagc agtttcccag tttgccgagt ctcgccagat
     3481 tcttcagttt tcccagtagt cccgctggcg gcagaagata gctggctatc gttattcccc
     3541 ccaggatcat tctctgccac ggcgggaact ccggctggat atccagatat gtgacctgct
     3601 caccgccaat aaagacgtcc ggcgaacctc ccacgatcgc cgcactgcac tccagtttgt
     3661 cgcccttgcg cgcggcaggc tgattgttga taaagacgtt gctgctgccc tgggctatcc
     3721 tcggtggttt gttctccttg tcgcagagcg ccgtgtcctc accggcttcc gggaaatcca
     3781 catctttttt gatttcgcca cgcccgccca ccatggtggc aaactccatt aataccggac
     3841 cggcgggaaa cagaagcgat gcgccggcgc tgacgcgatc cataaaaccg gcattgctgt
     3901 ccgtcagggt gataacagcg ttcccccagg ccttcgccag tggtactgct ttttccgctg
     3961 ccgattcaag caggtcagtg gcaatatcac cccatgaggg ttccccctgt gggacttcag
     4021 gtattgctcc aggtggggga ggagggggaa gggtgaccgc tgcacgtgcg gccagctcgc
     4081 cgttaatttt tacgttttca gaaccggtat tgatggcgcc gtccgtgtca caaaagctgt
     4141 ttatccatga ttcaagctgg ttctgtttct cctccacata atcactcgcc ttatagctgg
     4201 catacatcag cgcaggtacc agaaccagcg cataagggcc ggtggcacag gcggccataa
     4261 tcaggcaacc ttctgcgatg gtcactatcg cccccagtac ggcccctgcc agcgccccca
     4321 aaaaactctt gtgctgaatt tcatcgccca gcctggcggc gggggggcca gacttttttg
     4381 atgccaacat ctgtgcaatg gctttgtcgt tggctgcccg tttcatctgg ctggcgacca
     4441 tcgcctgctg tgacagcatt cgcattgcca gcgtaccgcc tgcggtcgct ccctgtggcc
     4501 cgcctggtcc ttcactcatg tgttatccct cgtttatgtc agcgcggctg gtccttttgg
     4561 atgtcattct ccggtggcaa cggggtgaag ctctttatca cctccagcag catcgtcttt
     4621 tgactgttct tcagggataa ggaggtgctg aaagtgaagg tcagcagcgt atctccacgc
     4681 cgctggaata cggctatctg gctaatccgc ccctgattcc cctcatcttc cggaccgttc
     4741 caggaataat ccagcagcca ggccggggcc tctgccagag tgatttcccg gcaggcatgt
     4801 tcggtgtacc catcaagttt gccctggtac agcgccagcg tttcctggta cagctctggt
     4861 gtcagggttc cctgtggaac ggcttcgcgg gtaatgacca gattgatgcc actgttatct
     4921 gtggacacca ggacctgcat tgactgatcc tgccattgag acggcagttg aatgtggcct
     4981 tcattaaaag tgaactgcat aaaatactcc ctgataatct gagtgaatca gttgatgaaa
     5041 acagaaccgc ccacgatggt gatgccatcc ttcgacagcc tgattaccgt cgattccccg
     5101 acctgtagct gaatgctatc cggcgtcagc gcaattttgc tttcctccac ggccagcaaa
     5161 atttccttaa tcgccgtata agcctgtata ccgtcgcctt cgccaccatt gtttacagcg
     5221 tcaacatgca ccgaaccggc aatcgtgcct ctgtcctgaa agacatcctc agataaagac
     5281 cctttcagta ctccggtgat cttgtggcct ttttccaccg taagaaggcg gttaccttcc
     5341 tggatcgtga ccgtctggtc attttttacg gtttcgcggt gatcgtgttt tacggtggtg
     5401 gtacggtcgt tgagcacttc ggtatccatg ttcttctgcg cgtggatgta gacctgttct
     5461 ctccctgtcg catcatcaaa cttcagctca ttaaatccgc tgcccatgta cgttttggag
     5521 cggatggtca tctgcgtctt tgttcccggc aggctgccgg gggggcggtt ttcctggtgg
     5581 taggtgcgcc ccataatgat cggctggtcc ggatcgccgt tgaggaagtc tacaatcact
     5641 tcctgaccca cacgcggtat cgccaggtgg ccaaaaccgg tgcctgccca cgcctgtgcc
     5701 acacggatcc agcacgaact gtcttggtct gccgggttat aacggtccca gttgaacttc
     5761 acccgcacgc gaccatgttc gtcgcagaag atttcctcgc ctgccggtcc tgtcacgaca
     5821 gcgctctgcg ggccgtcgac cagcggtttc agcaacggct gtggtcgcca tgttctgtct
     5881 gccgggatca ccgcaaaatg gttctccagc gccgtccccg ctccctgccg tcctggcacc
     5941 gcctgtggct gttcgccgtg cagttcactt gccaccacct gccattcccg gttcaggttc
     6001 gcctgcggat gccccgtcag cacaattcgt cgtcccggcc atatctccgg cgagcgactc
     6061 attccccgcg cggtttctgc attgtttcgc cagccgtcca tctgccagcg ggcaaagttc
     6121 tgcccgtgag cacccttgaa acgtccgggg tagtcataca cttcatactg cgtgcgctgg
     6181 tagtcctggt gctggccttc ctgttcaaaa cgtccggccc agccggggcg tttaaaggtg
     6241 tagtctttgg tcaccacgga agaagggcgg atttgtgcgc tgtagcggaa ctggctgatg
     6301 cagagggtgc tcacctcggt acgggtgttc gggttccatg ggatttcaaa agattcgggc
     6361 agatggcgga ctgtgtcgca cagcaccagg ctctggtcgg tacttttgta agcatgctcc
     6421 tcataaaaga agatgccttc ctccgccgcc atccggcaca ggaaatcgta atcagtctca
     6481 ccgtactgga cacaaaactc acgggaagga tgcggctcgc tgaacagcgg actccattcg
     6541 gtcaccccgt tttcctgcaa catcgtgccg aggatgcttt tgatgtcctc gttctggaag
     6601 atacggaagt tctggcgcag accggcacgc cacagcggcg ggtgcacctt catgctgtac
     6661 agcatctggt ttttgtcgtt ctccccgagt tcaaaccagg tcaccacgcc tttcacccgg
     6721 cgctgtactt catcgccctg ccatatcgtc aggtaggcca ttttgtccag cacctgcgca
     6781 aattcaaggg agagaaactg ctggctgacc agggagagat cgagggaaaa gagcgaagag
     6841 agtgactggt tcagatgaaa ggaaaccacc gcaaaagcgt ccggcggcag gccgtccact
     6901 tccagtgtga aacgtaatcc ggttgacata gcaacctcca gtaaaatgaa taatgatccg
     6961 taccgtacaa ctgcggataa tgtgataagc gggacgactt cagacgttat tagggaagga
     7021 acagaaaatg ctatggttat gtgagaattt tgtttaaata ttactttaca gaaacgggtg
     7081 tctgttcagt caaaaaaata atcacaagct caacctcttg tttcttggcg ttattcagga
     7141 caggtacgca tatcacaacg agtaacaaaa acgcaaagtt aaaccaatat tcataatata
     7201 tttttattca cagcaataaa taatccaatt aacattatta aaatgaatga ttgcataaga
     7261 aatatatgct accgctccac cctgataaca tgatctgcac attgcacatc ttaacgttgt
     7321 taaatactgg ctcaatagtg gatattttaa attaggaatt tatcaaattc acttgacaaa
     7381 tagattgagt gaataatata acaattaatg atgattaatc gcgattaaat tattcattgc
     7441 tttatatgat agatggcttg tttataacga tgaaaattta gaaagggcat tgtgatctac
     7501 aattggaaga aacacagatt taactaagtt tcatatacaa acatctcgtg aaactttgaa
     7561 aaataaaagc cttcgatatt tcatatgttt aataagtttt tctctaattt gttgatcaac
     7621 aataccagga ggtgatgcac cacgccctga atttgaactt aataatcact ctctctccgg
     7681 cagcataatc acaaaccttg tcccccgcgc atctgacgtt actgcaaccg tgcctttatg
     7741 cgcgacaaca atcgatttca ctatcgccag cccaatgccg ctaccttcgc cttttcgctg
     7801 gcgggaaggg tcaacgcggt agaaacggtc aaacaatcgc ggtaagtgct cgggcgcaat
     7861 gggcgtaccg gggttttcga cggtaacttg cacctggtga tcgaccgtct gacagcgcac
     7921 tacaattgtc tctctggttg gcgtataacg cagggcatta gaaagcaggt tgcttaacgc
     7981 ccgacgcagc atcagcggat cgcccgcaac ctgacatttg tcgccaacaa atcgcaactc
     8041 cacgccgcga tcttccgcta acgcctcgaa aaaatcgaac actttgctga cttcatccgc
     8101 caggttgagc attttctttt cggggattag ctggttgtta tcggcctgag cgagaaacag
     8161 catatcgctg accattttcg ccattcgcgt cagctcttcg agattagagt agagcacatc
     8221 ttccagctcc ttctggctgc gagactggct gagggcgatt tccgtttgcg taatgagatt
     8281 ggtaatcggc gtgcgaattt cgtgagcgat atccgctgag aaattggact ggcgggtaaa
     8341 gacatcctca atacgctcga tcatatggtt gaacgacagt accagctgtt ccagctcaat
     8401 gggctcggtc tgcgggtcaa ggcgaacgtc gagatctttc gaggtgatat tctggatttg
     8461 acggctgacg ctgcggatcg gcgcgtgacc tttatgtacc gccaa
//
LOCUS       DAJVRF010000155         8430 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJVRF010000155 DAJVRF010000000
VERSION     DAJVRF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.155"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            3..335
                     /gene="csgC"
                     /locus_tag="OQN13_003420"
     CDS             3..335
                     /gene="csgC"
                     /locus_tag="OQN13_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCS1616692.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
     gene            456..767
                     /locus_tag="OQN13_003421"
     CDS             456..767
                     /locus_tag="OQN13_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1616693.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            862..1395
                     /gene="ymdB"
                     /locus_tag="OQN13_003422"
     CDS             862..1395
                     /gene="ymdB"
                     /locus_tag="OQN13_003422"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCS1616694.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1337..2818
                     /gene="clsC"
                     /locus_tag="OQN13_003423"
     CDS             1337..2818
                     /gene="clsC"
                     /locus_tag="OQN13_003423"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCS1616695.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2826..3983)
                     /gene="mdoC"
                     /locus_tag="OQN13_003424"
     CDS             complement(2826..3983)
                     /gene="mdoC"
                     /locus_tag="OQN13_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCS1616696.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4378..5913
                     /gene="mdoG"
                     /locus_tag="OQN13_003425"
     CDS             4378..5913
                     /gene="mdoG"
                     /locus_tag="OQN13_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCS1616697.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5906..>8430
                     /gene="mdoH"
                     /locus_tag="OQN13_003426"
     CDS             5906..>8430
                     /gene="mdoH"
                     /locus_tag="OQN13_003426"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCS1616698.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKP"
BASE COUNT         2122 a         2009 c         2198 g         2101 t
ORIGIN      
        1 atatgaatgc gttattactc cttgcggcac tttccagcca gataaccttt aatacgaccc
       61 agcaagggga tgtgtatacc attattcctg aagtcactct tactcaatct tgtctgtgca
      121 gagtacaaat attgtccctg cgcgaaggca gttcagggca aagtcagacg aagcaagaaa
      181 agaccctctc attgcctgct aatcaaccta ttgctttgac gaagttgagt ttaaatattt
      241 ccccggaaga tcgggtgaaa atagttgtta ctgtttctga tggacaggca cttcatttat
      301 cacaacaatg gccgccctct tcagaaaagt cttaatttgt tgaaatatcg agcataagat
      361 gaatctggag agaatggtct gctgcgaatc agccaacctg aaagtatgga taacacaacc
      421 ctcaaggatg actaatcatt gaggaaatag aataaatgtt cagacctttt ttaaactctc
      481 ttatgctcgg cagtttgttt tttcctttta ttgccatcgc aggaagcacc gcgcaagggg
      541 gtgtgatcca tttttatggc caaattgtgg aaccggcatg tgacgtcagc acccagtcat
      601 cacccgtaga aatgaactgc ccacagaatg gttctgttcc gggcaaaacc tattccagca
      661 aagcgttaat gagcggtaat gttaaaaacg cgcaaatagc ctcagtaaaa gtgcagtatc
      721 ttgataaaca gaaaaagctg gctgtaatga acatcgaata taactaagtt tctgaacaac
      781 gcacgttgct gagcagaaaa atgcgattta accaaaaagc ctgctgtaca cttaagaaac
      841 aagaaggtgt aaggaggcgt tatgaaaacg cgtattcatg ttgtgcaggg tgatattacc
      901 aaactggccg ttgatgtgat tgtgaatgcg gctaatccgt cattaatggg aggcggcggc
      961 gtcgatgggg ccattcatcg cgcagcgggt ccggccctgc tggatgcctg tttaaaagtc
     1021 aggcaacagc agggcgattg ccctacgggg catgccgtta ttacgcttgc aggcgatctt
     1081 cccgctaaag ccgtagtaca caccgtcggg ccagtctggc gtggtggtga acaaaacgaa
     1141 gaccagcttt tgcaggatgc ctatctgaat agcctgcgac tggtggcggc aaacagctat
     1201 acgtcagtgg cttttcctgc aatcagtact ggggtttatg gttaccctcg tgcggcagcg
     1261 gctgaaatcg cagttaaaac cgtttcagaa tttattaccc gtcacgcttt acccgaacag
     1321 gtatactttg tctgttatga tgaagaaaac gcccacctct acgaaagact ccttacccaa
     1381 caaggagatg aatgatttgc cccggctggc gagcgcggtg ctgccactgt gttcgcaaca
     1441 tcccggtcag tgtggtcttt tccctctgga gaaaagtctg gatgcgtttg ccgcccggta
     1501 tcgtctggcc gaaatggcag agcatacgct ggatgttcag tattacatct ggcaggacga
     1561 tatgtcgggt cggttactgt tttccgccct gttagccgca gcaaagcgtg gcgttcgcgt
     1621 ccgtttgttg ctggacgaca acaatacgcc cggacttgac gatattttac gcttgcttga
     1681 cagccatccc cgcattgaag tccggctttt taatcctttc tcgtttcgct tgctgcgtcc
     1741 gcttggttat atcaccgact tttcccgtct caatcgccgt atgcacaata aaagtttcac
     1801 tgtcgatggc gtggttaccc tggtgggagg acgaaatatt ggtgatgcct attttggggc
     1861 aggggaagag ccactttttt cggatttaga tgttatggca ataggacccg tggtagagga
     1921 cgttgccgat gatttcgccc gctactggta ttgcaaatcg gtttcaccct tacagcaggt
     1981 gctggatgtc ccggaaggtg aaatggcgga tcgcatcgag ttacccgcct cctggcataa
     2041 cgatgccatg acgcatcgtt acttacgcca aatggaatcc agtccgttta taaatcatct
     2101 ggttgatgga acgttgccgc ttatctgggc gaaaacacgt ttattaagtg atgatccggc
     2161 gaaaggggag ggcaaggcaa aacggcattc actgttaccg cagcgcctgt ccgatatcat
     2221 gggctcaccc agtgaacgca tcgatattat ctcttcctat tttgtaccga cacgcgcagg
     2281 tgtggcgcaa ctcttacgga tggtgagaaa aggggtaaag attgcgatcc taaccaattc
     2341 tcttgccgct aacgatgttg ctgtcgtcca tgccggatac gcgcgctggc gcaaaaaatt
     2401 gctccgctat ggcgtggaat tatatgaact caagccgacg cgtgaacaaa gtagtacgtt
     2461 acacgatcgc ggcataaccg gtaattccgg agccagcctg catgctaaaa cttttagcat
     2521 cgatggtaaa acggtgttta tcggctcttt caatttcgat ccgcgttcaa cattgctcaa
     2581 tactgaaatg ggcttcgtga tagagagcga aacgctggca cagttaattg ataaacgctt
     2641 tattcagagc cagtatgatg cggcctggca gctccgtctg gacaggtggg gacggatcaa
     2701 ctgggttgat cgtcatgcaa agaaagagat tattctcaaa aaagaacccg ccaccagttt
     2761 ctggaagcgg gttatggtca gactggcgtc gatattgccc gtggaatggt tattgtaaat
     2821 gtggcttaac gggctggtgc tttatcgttc tcacgcttaa caaccggttt accagaaaac
     2881 aaaaacttca gtaacgggat gcgtaaatga atttcataca gaattatcgc aatccctact
     2941 acgaatatca ggccacagag aaaaccaagc cagttggagg tgatgtgcgg tgtaatgtat
     3001 gcgccgaaaa acagcgttaa cgggtggtga accagataga taaatagcga tgcgttaaca
     3061 aagtaagtca cccgcgctga ctggaagtta agcaaacggt ggccaaagga gaagaccaca
     3121 ttcaccatcc acagaccgag gaccatggtg atcaccgact cggtttcgta catccaggca
     3181 tcgccactgc catagcgctg gttgagtaaa taagcgacaa atgccaatgc tgctgcaagg
     3241 gtacagccac gagacggcgt ggtaaacaag gctttaagat gagggaaaat gaaagccagt
     3301 gcaccgagga taaagaacgg caaataaaat agcgtttgca tgacaataaa attgaacatg
     3361 ccattactca gaatgggcgg atacacaata aaaatcgttc ttcttattac cgcatagccg
     3421 atgccgaggc ataaaaaaat caccgatagt tttaccatcg agaatttttt attcgtttta
     3481 tcagaatttt ctaaattatt tctgatgcgt ttaaatatcc atacgcacag cgtcgtcatg
     3541 actactaaca ccagtaaaaa ccacaggtgt gatattaatt cccaggccaa cgtattatat
     3601 ttgtcataca atgacagccc aggccaactt tccgctttcc ctttgacgta ttgcagcata
     3661 ataaattgcg gcaatgtcag taaggggatg gctgttaaca tcgggatacc tacacgttcg
     3721 acacgtactt tccaccattt tttcaaggga tagcgtaaaa aaagcatgta ggaaaagtag
     3781 ccggatataa cgaaaaatac ctgcatgcgg aacgagtgga tgaagtcatt aaaaagggtc
     3841 agccacaatg acggttcggc gctattcaca tgccatgtat ggctcgaata gattaaagaa
     3901 atatgaaaag ggatccctaa caacatcagc caggcgcgga tggagtcgag gaaatattca
     3961 cgttgcgcgg gtactggatt catatatggt taactaatct cggatttttc gtcttatccc
     4021 tgtcgggtta tgcctttagg cttgttgcca tagcgacacc gacctgaccg cgccaggcgc
     4081 aggcttcaag gtttttatgc atagcatcat cgctaccact aaccagaatg gaagcgtctg
     4141 taagacggtt gataaataaa attgctggca aaccctacac gaagtcgatg cttctgtctt
     4201 taggagaagc acggaaagtg aaaacggttg caatcaggtg cttaatccat gagccagcgt
     4261 gctgaacgat accgggattc tgttgtcgga atggctggtt atccattaaa atagatcgga
     4321 tcgatataag cacacaaagg gggaagtgct ttactaatta tgaaacataa actacaaatg
     4381 atgaaaatgc gttggttgag tgctgcagta atgttaaccc tgtatacatc ttcaagctgg
     4441 gctttcagta ttgatgatgt cgcaaagcaa gctcaatcct tagccgggaa aggctatgag
     4501 gcgcccaaaa gcaacttgcc ctccgttttc cgcgatatga aatacgcgga ctatcagcag
     4561 atccagttta atcatgacaa agcgtactgg aacaatctga agaccccatt caaactcgag
     4621 ttctaccatc agggtatgta cttcgatacc ccggtcaaaa taaatgaagt gactgccacc
     4681 gcagtcaaac gaatcaaata cagcccggat tatttcactt tcggcgatgt tcagcatgac
     4741 aaagacacgg taaaagacct tggttttgcc ggttttaaag tgctttaccc gatcaacagc
     4801 aaagataaaa acgatgaaat cgtcagcatg ctcggggcca gctatttccg cgtgattggt
     4861 gcaggtcagg tttatggcct ttctgcccgc ggcctggcaa ttgataccgc cttgccatcg
     4921 ggggaagaat ttccacgctt caaagagttc tggatcgagc gtccaaaacc gactgataaa
     4981 cgtttaacca tttatgcatt gcttgactcg ccgcgcgcga caggtgctta caaattcgtg
     5041 gttatgccag ggcgtgacac ggttgtggat gtgcagtcga aaatctatct gcgcgataaa
     5101 gtcggcaaac tgggggttgc accgttaacc agtatgttcc tgtttgggcc gaaccaaccg
     5161 tcgcctgcaa ataactatcg tccggagttg cacgactcta acggtctctc tatccatgcc
     5221 ggtaatggcg aatggatctg gcgtccgttg aataacccga aacatttagc ggttagcagc
     5281 ttctcgatgg aaaacccgca aggcttcggt ctgttgcagc gcggtcgtga tttctcccgc
     5341 tttgaagatc tcgatgatcg ttacgatctt cgtccaagcg catgggtgac tccgaaaggg
     5401 gagtggggta aaggcagcgt tgagctggtg gaaattccaa ccaacgatga aaccaacgat
     5461 aacatcgtcg cttactggac gccggatcag ctgccggagc cgggtaaaga gatgaacttt
     5521 aaatacacca tcaccttcag ccgtgatgaa gacaaactgc atgcgccaga taacgcatgg
     5581 gtgcaacaaa cgcgtcgttc aacgggggat gtgaagcagt cgaacctgat tcgccagcct
     5641 gacggtacta tcgcctttgt ggtcgatttt accggcgcag agatgaaaaa actgccagag
     5701 gataccccgg tcacagcgca aaccagcatt ggtgataatg gtgagatagt tgaaagcacg
     5761 gtgcgctata acccggttac caaaggctgg cgtctggtga tgcgtgtgaa agtgaaagat
     5821 gccaagaaaa ccactgaaat gcgtgctgcg ctggtgaatg ccgatcagac gttgagtgaa
     5881 acctggagct accagttacc tgccaatgaa taagacaact gagtacattg acgcaatgcc
     5941 catcgccgca agcgagaaag cggcattgcc gaagactgat atccgcgccg ttcatcaggc
     6001 gctggatgcc gaacaccgca cctgggcgcg ggaggatgat tccccgcaag gctcggtaaa
     6061 ggcgcgtctg gaacaagcct ggccagattc acttgctgat ggacagttaa ttaaagacga
     6121 cgaagggcgc gatcagctga aggcgatgcc agaagcaaaa cgctcctcga tgtttcccga
     6181 cccgtggcgt accaacccgg taggccgttt ctgggatcgc ctgcgtggac gcgatgtcac
     6241 gccgcgctat ctggctcgtt tgaccaaaga agagcaggag agcgagcaaa agtggcgtac
     6301 cgtcggtacc atccgccgtt acattctgtt gatcctgacg ctcgcgcaaa ctgtcgtcgc
     6361 gacctggtat atgaagacca ttcttcctta tcagggttgg gcgctgatta atcctatgga
     6421 tatggttggt caggatttgt gggtttcctt tatgcagctt ctgccttata tgctgcaaac
     6481 cggtatcctg atcctctttg cggtactgtt ctgttgggtg tccgccggat tctggacggc
     6541 gttaatgggc ttcctgcaac tgcttattgg tcgcgataaa tacagtatat ctgcgtcaac
     6601 agttggcgat gaaccattaa acccggagca tcgcacggcg ttgatcatgc ctatctgtaa
     6661 cgaagacgtg aaccgtgttt ttgctggcct gcgtgcaacg tgggaatcag taaaagccac
     6721 cgggaatgcc aaacactttg atgtctacat tcttagtgac agttataacc cggatatctg
     6781 cgtcgcagag caaaaagcct ggatggagct tatcgctgaa gtcggtggcg aaggtcagat
     6841 tttctatcgc cgccgccgtc gccgcgtgaa gcgtaaaagc ggtaatatcg atgacttctg
     6901 ccgtcgctgg ggcagccagt acagctacat ggtggtgctg gatgctgact cggtaatgac
     6961 cggtgattgt ttgtgcgggc tggtgcgcct gatggaagcc aacccgaacg ccgggatcat
     7021 tcagtcgtcg ccgaaagcgt ccggtatgga tacgctgtat gcgcgctgtc agcagttcgc
     7081 gacccgcgtg tatgggccac tgtttacagc cggtttgcac ttctggcaac ttggcgagtc
     7141 gcactactgg ggacataacg cgattatccg cgtgaaaccg tttatcgagc actgcgcact
     7201 ggctccgctg ccgggcgaag gttcctttgc cggttcaatc ctgtcacatg acttcgtgga
     7261 agcggcgttg atgcgccgtg caggttgggg ggtctggatt gcttacgatc tcccgggttc
     7321 ttatgaagaa ttgccgccta acttgcttga tgagctaaaa cgtgaccgcc gatggtgcca
     7381 cggtaacctg atgaacttcc gtctgttcct ggtgaagggt atgcacccgg ttcaccgtgc
     7441 ggtgttcctg acgggcgtga tgtcttatct ctccgctccg ctgtggttta tgttcctcgc
     7501 gctctctact gcattgcagg tagtacatgc gttgaccgaa ccgcaatact tcctgcaacc
     7561 acggcagttg ttcccggtgt ggccgcagtg gcgtcctgag ctggcgattg cactttttgc
     7621 ttcgaccatg gtgctgttgt tcctgccgaa gttattgagc attttgctta tctggtgcaa
     7681 aggaacgaaa gaatacggcg gcttctggcg cgttacatta tcgttgctgc tggaagtgct
     7741 gttttccgtg ctgctggctc cggtacgcat gctgttccat acggtcttcg tcgtcagcgc
     7801 gttccttggc tgggaagtgg tgtggaattc accgcagcgt gatgatgact ccacttcctg
     7861 gggtgaagcg ttcaaacgcc acggctcaca gctgctgtta gggttagtgt gggctgttgg
     7921 gatggcgtgg ctggatctgc gtttcctgtt ctggctggca ccgattgtct tctcgttgat
     7981 cctgtcaccg tttgtttcgg tgatttccag ccgtgccacc gttggtctgc gcaccaaacg
     8041 ctggaaactg ttcctgatcc cggaagagta ttcaccgccg caggtgctgg ttgataccga
     8101 tcggttcctt gagatgaatc gccaacgctc ccttgacgat ggctttatgc acgcagtgtt
     8161 taacccgtca tttaacgctc tggcaaccgc aatggcgacc gcgcgtcacc gcgccagtaa
     8221 ggtgctggaa atcgcccgtg accgccacgt tgaacaggcg ctgaacgaga cgccagagaa
     8281 gctgaatcgc gatcgtcgcc ttgtgctgct aagcgatccg gtgacgatgg cccgtctgca
     8341 tttccgcgtc tggaattccc ctgagagata ttcttcatgg gtgagttatt acgaagggat
     8401 aaagctcaat ccactggcat tgcgtaaacc
//
LOCUS       DAJVRF010000156         8426 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJVRF010000156 DAJVRF010000000
VERSION     DAJVRF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.156"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..83)
                     /locus_tag="OQN13_003427"
     CDS             complement(<1..83)
                     /locus_tag="OQN13_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCS1616699.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGH"
     gene            375..896
                     /gene="fecI"
                     /locus_tag="OQN13_003428"
     CDS             375..896
                     /gene="fecI"
                     /locus_tag="OQN13_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCS1616700.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            893..1846
                     /gene="fecR"
                     /locus_tag="OQN13_003429"
     CDS             893..1846
                     /gene="fecR"
                     /locus_tag="OQN13_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCS1616701.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1933..4257
                     /gene="fecA"
                     /locus_tag="OQN13_003430"
     CDS             1933..4257
                     /gene="fecA"
                     /locus_tag="OQN13_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCS1616702.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMNAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            4302..5204
                     /gene="fecB"
                     /locus_tag="OQN13_003431"
     CDS             4302..5204
                     /gene="fecB"
                     /locus_tag="OQN13_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCS1616703.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5201..6199
                     /gene="fecC"
                     /locus_tag="OQN13_003432"
     CDS             5201..6199
                     /gene="fecC"
                     /locus_tag="OQN13_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCS1616704.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6196..7152
                     /gene="fecD"
                     /locus_tag="OQN13_003433"
     CDS             6196..7152
                     /gene="fecD"
                     /locus_tag="OQN13_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCS1616705.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7153..7920
                     /gene="fecE"
                     /locus_tag="OQN13_003434"
     CDS             7153..7920
                     /gene="fecE"
                     /locus_tag="OQN13_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCS1616706.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
BASE COUNT         1843 a         2411 c         2344 g         1828 t
ORIGIN      
        1 tgatgtccgg cagtgctttt gccgttacgc accacgcctt cagtagcgga gcaggaagga
       61 catctgatgg aaatggaagc cacgcaagca ccttaaaatc accatcatac actaaatcag
      121 taagttgaca gcattaccga aatccctatc ctttagggca gtgaggatgt caataatagc
      181 agaccacatc atgagtcatt ctgaccgaca catgccagaa gcagaggctc catcccattt
      241 tatacctacc ttataacact tagaaaaaca acatgttaaa aatgtctatt ggaaacaatt
      301 ttatttccaa ttgtaatgat aactattctc atattaatat ggctacgtga taattaactt
      361 ttgatgcact ccgcatgtct gaccgcgcca ctaccacagc ttccttaaca ttcgagtcgc
      421 tttatggcac acatcacggc tggttgaaaa gctggctgac gcgcaaactc cagtctgctt
      481 ttgatgcaga tgacattgcc caggacactt ttttgcgggt aatggtcagc gaaacgctct
      541 cgacgatccg cgatcctcgc tccttcctct gcactatcgc caaacgcgtg atggtggacc
      601 tgtttcgccg aaacgcgctg gaaaaagcgt atctggagat gctggcgctt atgccggagg
      661 ggggagcgcc ttcacctgag gaacgcgaaa gccaactcga gaccctacaa ctcctcgaca
      721 gcatgctgga cgggctaaac ggcaaaacac gtgaagcgtt tctgctttcg caactggatg
      781 gtctgacata cagcgagatt gcgcacaaac tcggtgtttc catcagctcc gtgaaaaaat
      841 acgtggcgaa agccgtcgag cactgcctgc tgttccgtct ggagtatggg ttatgaatcc
      901 tttgttaacc gattcccgcc gtcaggcgct gcgttcagct tcccactggt atgccgtgct
      961 aagcggcgag cgcgtcagcc cacaacagga agcgcgctgg caacagtggt atgaacagga
     1021 tcaggataac cagtgggcct ggcagcaggt tgaaaacctg cgcaaccagc ttggcggtgt
     1081 gcctggcgac gttgccagcc gggcgttgca cgatacccgc ctcacccgcc gtcacgtgat
     1141 gaaaggatta ctgctgttgc tcggcgctgg cggaggctgg cagctctggc agtcggaaac
     1201 cggcgaaggt ctgcgggcag attaccgcac cgccaaaggc acagtcagcc gtcagcaact
     1261 ggaagatggc tccctgctca cgctgaatac ccaaagcgcg gcggatatgc gttttgatgc
     1321 gcatcagcgc accgtccggc tctggtacgg tgaaatcgcc attactaccg cgaaagatgc
     1381 cctgcaacgc cccttccgtg tcctgacccg tcagggccag ctcactgctt tagggacaga
     1441 atttaccgtc cgccagcagg ataatttcac gcagcttgac gtgcagcagc acgccgtgga
     1501 agtgcttctc gccagtgccc ccgcgcaaaa acgcatcgtg aacgctggtg aaagcctgca
     1561 gttcagcgcc tctgagtttg gcgcagtgaa accgctggat gacgagagta caagctggac
     1621 gaaggacatc ctgagcttca gcgataaacc gctgggtgag gtgatagcca cgctaacccg
     1681 ttaccgcaac ggcgtgctgc gctgcgatcc cgccgttgcc gggctgcgcc tgagcgggac
     1741 gttcccgctg aaaaataccg atgcgatcct gaacgttatc gcgcaaacgc ttcccgttaa
     1801 aattcagtct attacgcggt actggataaa catttcacca ctgtaaggaa aataattctt
     1861 atttcgattg tcctttttac ccttctcgtt cgactcatag ctgaacacaa caaaaatgat
     1921 gatggggaag gtatgacgcc gttacgcgtt tttcgtaaaa caacaccttt ggttaacgcc
     1981 attcgcctga gcctgctgcc gctggccggt ctctcgtttt ccgcttttgc tgcacaggtt
     2041 aatatcgcac cgggatcgct cgataaagcg ctcaatcagt atgccgcaca cagcggattt
     2101 accctctcgg ttgacgccag cctgacgcgc ggcaagcaga gcaacggcct gcacggcgat
     2161 tacgacgtcg agagcggcct gcaacaactg ctggacggca gcggactgca ggtaaaaccg
     2221 ctgggaaata acagctggac gctggagccc gcgcccgcac caaaagaaga tgccctgacc
     2281 gtggtcggcg actggctggg tgatgcgcgt gaaaacgacg tatttgaaca tgctggcgcg
     2341 cgtgacgtga tccgccgtga ggatttcgcc aaaaccggcg caaccaccat gcgtgaggta
     2401 cttaaccgca tccctggcgt cagcgcgccg gaaaacaacg gcaccggcag ccacgacctg
     2461 gcgatgaact ttggcatccg gggcctgaac ccgcgcctcg ccagccgctc gaccgtcctg
     2521 atggacggca tccccgtccc cttcgcccct tacggtcagc cgcagctttc actggctccc
     2581 gtttcgctcg gcaacatgaa tgccattgac gtggtacgcg gtggtggtgc ggtgcgttac
     2641 ggaccgcaga gcgtgggcgg cgtggtgaac tttgttaccc gtgccattcc gcaggacttt
     2701 ggtatcgagg cgggcgtgga aggtcagctc agcccaacct cttcacaaaa caacccgaaa
     2761 gagacgcaca acctgatggt gggcggcaca gcggacaacg gttttggcac cgcgctgctc
     2821 tactccggca cgcgcggcag tgactggcgc gagcacagcg ccacccgcat cgacgacctg
     2881 atgctgaaaa gcaaatatgc gccggatgag gtgcacacct tcaacagcct gctgcaatat
     2941 tacgacggtg aagccgacat gcccggtggc ctgtctcgcg cggattacga cgccgatcgc
     3001 tggcaatcca cccgcccgta tgaccgcttc tggggtcgtc gcaagctggc gagcctgggc
     3061 taccagttcc agccagacag ccagcataaa ttcaacattc aggggttcta cacccaaacc
     3121 ctgcgcagcg gctacctgga gcaaggcaaa cgcatcaccc tctcgccgcg taactactgg
     3181 gtgcgcggta ttgagccacg ctacagccag atctttatga tcggcccttc cgcgcacgaa
     3241 gtgggcgtgg gctatcgcta tgtgaatgaa tcaacgcatg aaatgcgtta ctacaccgcc
     3301 accagcagcg ggcagttgcc gtccggctca agcccttacg accgcgatac gcgttccggc
     3361 accgaggcgc acgcctggta tctggatgac aaaatcgaca tcggcaactg gaccatcacg
     3421 ccgggtatgc gtttcgaaca tatcgagtca taccagaaca acgccatcac aggcacgcac
     3481 gaagaagtga gctataacgc accgcttccg gcgttgaacg tgctctatca cctgactgac
     3541 agctggaatc tttatgcaaa cactgaaggc tcgttcggca ccgtacagta cagccagatt
     3601 ggcaaggctg tgcaaagcgg caatgtggaa ccggaaaaag cgcgaacctg ggaactcggt
     3661 acccgctacg acgacggcgc gctgacggcg gaaatggggc tgttcctgat taactttaac
     3721 aatcagtacg actccaacca gaccaacgac accgtcactg cacgtggcaa aacgcgccat
     3781 accgggctgg aaacgcaggc acgttacgat ctgggtacgc taacgccaac gcttgataac
     3841 gtttccatct acgccagcta tgcgtatgtg aacgcggaaa tccgcgagaa aggcgacacc
     3901 tacggcaatc tggtaccatt ctccccgaaa cataaaggca cgctgggcgt ggactacaag
     3961 ccaggaaact ggatgttcaa tctgaacagc gatttccagt ccagccagtt tgcggataac
     4021 gccaatacgg tgaaagagag cgccgacggc agtaccggcc gcattcccgg cttcatgctc
     4081 tggggcgcac gcgtggtgta tgactttggc ccgcagatgg cagatctgaa cctggcattc
     4141 ggtgtgaaaa acatcttcga ccaggactac ttcatccgct cttatgacga caacaacaaa
     4201 ggcatctatg caggccagcc gcgcacgctg tatatgcagg gggcgttgaa gttctgatgt
     4261 gttaacgccc ggcttgccgg gcttttagct ggaatgtgat tatgttggca tttatccgtt
     4321 ttctttttgc aggcctgctg ctggtgatca gccacgcctt tgccgccacg gttcaggacg
     4381 aacacggcac gtttacactc gaaaaaacgc cacaacggat tgtggtgctg gaactctcgt
     4441 tcgccgatgc gctggccgcc gtggacgtca gcccgatcgg tattgccgac gataacgatg
     4501 caaaacgcat cctgcccgaa gtgcgtgcgc acctgaaacc gtggcagtcc gtcggaacgc
     4561 gcgcgcagcc gagcctggaa gccattgccg ctctgaaacc agacctgatc attgccgaca
     4621 gcagtcgcca tgcgggggtt tacatcgcct tgcagcaaat cgcgtcggta ctgctgctta
     4681 agtcccgcaa cgaaacctac gctgaaaatt tgcaatctgc ggctatcatc ggcgaaatgg
     4741 tgggtaaaaa gcgagagatg caggcacgtc tggaacaaca taaagagagg atggcgcagt
     4801 gggccagcca gcttcccaaa gggacacgcg tggcctttgg cacatcacgc gaacagcaat
     4861 tcaacctgca tactcaggag acctggaccg gcagcgtgct ggcctctctg gggctgaacg
     4921 ttcccgctgc gatggcgggt gcgtccatgc cgtccatcgg cctggagcaa ctgctggcgg
     4981 tcaatcctgc ctggctgctg gttgcccact atcgcgaaga gagcattgtt aaacgctggc
     5041 aacaagatcc gctctggcag atgttaaccg ccgcgcagaa gcagcaggtt gcttcggtcg
     5101 acagtaacac ctgggcgcgg atgcgcggta tttttgctgc agagcgtatt gccgctgaca
     5161 cggtaaaaat cttccaccat cagccgctta ccgttgtgaa atgaccgcga taaaacaccc
     5221 ggtgctgctg tgggggcttc ccgttgcagc acttattatt attttctggc tgagtctgtt
     5281 ttgctactcg gccattcctg tttccggagc agatgcaacc cgcgccctgc tgcctggaca
     5341 cacgccaacg ctaccagaag cgctggtgca aaaccttcgt ttgccacgaa gcctggtcgc
     5401 cgttctgatc ggcgcaagcc tggcgctcgc gggcacgctg ctgcaaaccc tgacccacaa
     5461 cccaatggcc tctccttcac tgctcggcat taacagcggc gcggcgctgg ctatggcgct
     5521 taccagcgcg ctgagtccga cgccgattgc aggctattct ctgtcgttca tcgcggcatg
     5581 cgggggcggc gtgagctggc tgctggtcat gaccgcagga ggcggatttc gtcataccca
     5641 tgacagaaac aaactgatcc tcgcgggtat cgcgctgtcg gccttttgta tgggccttac
     5701 ccgcatcacc ctgctgctgg ccgaagatca tgcttacggc atcttttact ggctggctgg
     5761 cggagtgtcc cacgcccgct ggcaggatgt ctggcagctc ttgccggtgg tggtcactgc
     5821 agtccctgtc gtgttgctgc tggcgaatca actgaacctg ctcaacctca gcgacagcac
     5881 cgcccatacg ctgggagtga acctgacgag gctacgtttg gtcatcaata tgttagtgct
     5941 gcttctggtt ggtgcgtgcg tcagcgtggc aggtccggtg gcgtttatcg gtctgctggt
     6001 gccacatctg gcgcgcttct gggcaggctt cgatcagcgc aacgtactgc cagtgagcat
     6061 gctgctgggg gccacgctga tgctgctggc agatgtactc gcacgcgcgc tggccttccc
     6121 cggagatctg cccgcaggcg cagtgctggc gctgattggc agcccttgct ttgtctggct
     6181 tgtgaggagg cgaggatgaa aattgcgctg gttattttca tcacccttgc cctggcgggc
     6241 tgtgcgctgt tatcactcca tatgggagtg atccccgtgc cgtggcgcgc gctgctgacc
     6301 gactggcagg ccggacgcga gcattattat gtattgatgg agtaccgact gccgcgcttg
     6361 ctgctggcac tgtttgtcgg tgcagccctc gccgtggcgg gcgtgctgat acaggggatt
     6421 gtgcgcaacc ctctggcatc accggatatt ctcggcgtta accatgccgc cagcctggcc
     6481 tctgtggggg ctctacttct tatgccgtca ctgcccgtga tggtgctgcc gctgctggcc
     6541 tttgcgggcg gcatggcggg gttgatattg ctgaagatgc tggcaaagac ccaccagccg
     6601 atgaagctgg cactcaccgg cgtggcgctt tctgcatgct gggccagcct gacggattat
     6661 ctgatgctct cgcgcccgca ggatgtgaac aacgccctgc tgtggctgac cggcagctta
     6721 tggggccgtg actggagctt tgtgaagatt gccatcccgc tgatgatttt atttctgccg
     6781 ctgagcctga gtttttgccg cgatctcgac ctccttgcac tcggcgatgc gcgcgccacc
     6841 acgctcggtg tgtcggtgcc ctatacccga ttctgggctt tgttactagc tgtcgccatg
     6901 acatctaccg gcgtggccgc ctgcggcccg attagcttta ttggtctcgt ggtgccgcat
     6961 atgatgcgta gcatcaccgg tggacgtcac cgcagactgc tgcctgtttc ggccctgaca
     7021 ggtgcgttgc tgttggtggt tgccgatctg ctggcgagaa ttattcatcc cccactggag
     7081 ctcccggttg gcgtgctgac cgccattatc ggtgcgccgt ggtttgtctg gttgcttgtg
     7141 agaatgcgat aaatgacttt acgaactgaa aatctgacgg tcaattacgg aacagacaag
     7201 gtacttaacg acgtttcact ctcactgcca acggggaaga tcaccgccct gatcggtcct
     7261 aacggttgcg ggaaatcgac gctgttaaac tgtttttcgc ggcttttaat gccgcagtct
     7321 ggcaccgtat ttctcggcga taatcccata aatatgctct catcgcgcca gttggcccgc
     7381 aggctttcgc tgctgcctca gcaccattta acgccagagg ggatcacagt ccaggagctg
     7441 gtttcgtatg gtcgtaatcc ctggctgtca ctctgggggc gtctctccgc tgaagacaat
     7501 gcacgagtta atgtcgccat gaaccagacc cggatcaatc atcttgccgt tcgtcggtta
     7561 accgagcttt ccggcggtca gcgccagcgc gcatttctgg caatggtcct ggcccagaat
     7621 acgcccgttg tattacttga tgagccaacc acctatcttg atatcaatca ccaggtggac
     7681 ctgatgcggt tgatgggcga actccggact caggggaaaa cggtggtcgc tgtgctgcac
     7741 gaccttaatc aggctagccg gtactgcgat caactggtgg taatggcaaa cggacatgtt
     7801 atggcgcaag gcacaccaga agaggtgatg accccaggat tgctgagaac agtattcagc
     7861 gtggaagcgg aaatacaccc cgagccggta tctggcaggc cgatgtgcct aatgaggtag
     7921 attgcacagg ccgtaagaac caaaccacga ctgaatgaaa ctggactggc gccagcaagc
     7981 ctgttcagac tggggctgaa cttttccgga ctctgaaaga ttaccaatac tcatcgtcca
     8041 tccgcttgct ttaggctgac aggttcataa tcaacgcaaa ccagagctgt acaggcttgg
     8101 gcgcggcttt caaaccagtc gtgatcacgg caatcaattt tgaactctgc ttaacggaca
     8161 tttctgtata acccttacgg caacgaaaaa cgcgaagtta aaattttaga aacccaaaaa
     8221 cgtgacatga ctaagtttag atttcagggg ggggagatca aaaaatttcg ctctgtgcca
     8281 gagcggacat tcacggagct ggttcattac caatgaggtt gggcttttga ggataaatca
     8341 atgatcagac gccaacgtaa atcaaaagca accctggaac ggaattgcta atccagtttc
     8401 tgaccatcga tttttctaaa aagtgt
//
LOCUS       DAJVRF010000157         8336 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJVRF010000157 DAJVRF010000000
VERSION     DAJVRF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8336
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.157"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(334..1170)
                     /locus_tag="OQN13_003435"
     CDS             complement(334..1170)
                     /locus_tag="OQN13_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS1616707.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(1170..1973)
                     /locus_tag="OQN13_003436"
                     /note="aph(3'')-Ib"
     CDS             complement(1170..1973)
                     /locus_tag="OQN13_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCS1616708.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCQFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(2034..2849)
                     /gene="sul2"
                     /locus_tag="OQN13_003437"
     CDS             complement(2034..2849)
                     /gene="sul2"
                     /locus_tag="OQN13_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS1616709.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            4038..4187
                     /locus_tag="OQN13_003438"
     CDS             4038..4187
                     /locus_tag="OQN13_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616710.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(4954..5394)
                     /locus_tag="OQN13_003439"
     CDS             complement(4954..5394)
                     /locus_tag="OQN13_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCS1616711.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            5612..6001
                     /locus_tag="OQN13_003440"
     CDS             5612..6001
                     /locus_tag="OQN13_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616712.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            6045..6233
                     /locus_tag="OQN13_003441"
                     /pseudo
     CDS             6045..6233
                     /locus_tag="OQN13_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(6200..7399)
                     /gene="tet(A)"
                     /locus_tag="OQN13_003442"
     CDS             complement(6200..7399)
                     /gene="tet(A)"
                     /locus_tag="OQN13_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCS1616713.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            7505..8155
                     /gene="tetR(A)"
                     /locus_tag="OQN13_003443"
     CDS             7505..8155
                     /gene="tetR(A)"
                     /locus_tag="OQN13_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCS1616714.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
BASE COUNT         1889 a         2295 c         2304 g         1848 t
ORIGIN      
        1 gggcgaggtc cagggcggcc gtcttcacgt tctgccttgc gcagatgaga tagatctagc
       61 gtggactcaa ggctctcgcg aatggctcgc gttggaaact ttcattgaca cttgaggggc
      121 accgcaggga aattctcgtc cttgcgagaa ccggctatgt cgtgctgcgc atcgagcctg
      181 cgcccttggc ttgtctcgcc cctctccgcg tcgctacggg gcttccaggg gtcgtttgcg
      241 ggaaggggcg gaatcctacg ctaaggcttt ggccagcgat attctccggt gagattgatg
      301 tgttcccagg ggataggaga agtcgcttga tatctagtat gacgtctgtc gcacctgctt
      361 gatcgcggcc gcgatagcta gatcgcgttg ctcctcttct ccatccgcgt tccaagctgc
      421 ggaaaggcac ccataagcgt acgcctggtc gagcaggcga cgcggatcga cgtccagcgc
      481 acgagagaat gcgtccgcca tctgtgcaat gcgtctagga tcgagacaaa ggtcgtctct
      541 gtcagccgga tcgtagaaca tattggcggc gccaaagccc acttcaccga ccagaccgac
      601 gggatctatc accagccagc cgcgactgga gaacatgatg ttttcatgat gcagatcgcc
      661 atgtagccca cgcagttccg aggcattgct catcatttga tcggctataa tcgccgcgtg
      721 gacgtagtca gtttgacaac ctgcgttttg atcatcgcgc gcccgctgaa acaaagctgc
      781 aaagcgatcc cggatcggga gaagggcaga aggcaggggt tcctcagatg cggcatacag
      841 cttcgccatt agttccgctg caatttcggt cgcctggtag tcgccgtgct cggcaacgat
      901 gtgagagagc attcgctccc cggcatattc gagcaacatc agattgttct cacgaccgag
      961 caaccggact gctcccctcc cattgcgcca taccagatag tcggccccgc gcagttcatc
     1021 agcaatgtct tctataggtt tcaatccctt gacgattgca ggagtcccgt ctggcaatga
     1081 aactttccaa acgaggctgg aaaaggtgtc cgcaatgaga acaggttgcg aaacgtgcca
     1141 atgagcagga aaaacaggcg gcatgaacat caaccccaag tcagagggtc caatcgcaga
     1201 tagaaggcaa ggcgttcgcg gtcgggggct tcgatcccca atacattgaa taggacagcg
     1261 aaggcgcgct ctgcttcatc tggcgctgcc cagttctctt cggcgttagc aatcatgagt
     1321 gccaaatcgg catagcgatc tgctgttccg agccgcccaa ggtcgatcag acccgtgcat
     1381 tgaagagttt tagggtccac catgaagttc ggcatgcagg gatcaccatg gcaaacaacc
     1441 atatcggtgc gctcttggtc gagccgcacc ggtagctctc gttcgacacg agccaaaaga
     1501 tcgagctgcg gcgtactctt gtcctcgtcc ggtaagaagt cgggattgac ggcattgcgg
     1561 gacaccacat caacggcgcg tccgaacatt cgcgacagcc tgcgctcaaa ctgacattga
     1621 tcaaccgata ggctgtgaac agcgccaagt tgctgcccca ttgacggcca cgctttgagc
     1681 aaatccgctc cagacagatc agccgccggt actcccggaa ttgccgttat caccaagcat
     1741 gcaccctcct gttcctcctg ccagttgatg acctcggggc aagccacacc tcgacctttg
     1801 agccaaatga ggcggtcacg ctctccagcg agctcaccgc ggcgggaagc aggtgcgatt
     1861 ttcgcgaagg catgcccgtc accacgtcga aaaacaaaat caccagattc tccgcctctg
     1921 acaggcaacc agtcagaatg cgattcacca aaaaaaatat tagttcgatt caatggaggt
     1981 tccttcagtt ttctgatgaa gcgcgaatat agagaaatat cccgaatgtg cagttaacga
     2041 attcttgcgg tttctttcag cgccgccaat accgccagcc cgtcgcgcaa ggggcgcggc
     2101 tcgtgtgtgc ggatgaagtc agctccacct gcggcggcgg caagctctgc agcgagtgtc
     2161 gcggccccga catcccccgg accacggcct gtgagcgcgc gcagaaagga tttgcgcgaa
     2221 acagacagaa gcaccggcaa atcgaagcgc agccgcaatt catcgaaccg cgccagcacc
     2281 gagagcgagg tttcgggagc agcccccaga aaaaacccca tgccgggatc aaggacaagg
     2341 cggttgcgtt tgataccggc acccgtcagc gccgcgatgc gcgcgtcaaa gaacgccgca
     2401 atgtgatcca tgatgtcgcc agcgggtgcc tcgcgccgat ctgcctgccc gtcttgcacc
     2461 gaatgcataa cgacgagttt ggcagatgat ttcgccaatt gcggatagaa cgcagcgtct
     2521 ggaaaaccgc gaatatcatt gagataggcc acaccacgcg acaaggcata ggcttgcgtc
     2581 gcgggttgat aactgtcgag cgagacggga atgccatctg ccttgagcgc gtccagcacc
     2641 ggcgcgatac gcgcgatttc tgtgtcggac gaaacaggcg cggcgtcggg attgctggat
     2701 gccggaccga ggtcgatcac atctgccccc tcggccatca gcttacgcgc ctgcgcaatg
     2761 gctgcgtctg gcgccagata ccggcctcca tcggagaaac tgtccgaggt tatgttgacg
     2821 atgccgaaaa tgatgagcga tttattcatg ggggcttcta taataataat aatcgagcat
     2881 gagtctcata cggatgctcg ggtcgaaagg gaatccccag gcgagtaacc tgtttgcggt
     2941 gatccgtttc atgatgatgt atctgtagca ttttggaaat aaatatatca tctctcaata
     3001 agtagatata tttatctttt ttgcttcttt ggtgctgcaa gtcaccaata cgagggaaac
     3061 ctgttacgga gcggtttaac cgtaatcaac agtgactggt gtcatgttcg gaagattaat
     3121 tgatagtaat ctacaaagga tcttcttgag atccttttca atgaatcgta atctcttgct
     3181 ctgtaaacga aaaaaccgcc tggggaggcg gtttgttcga aggttaagtc agttggggaa
     3241 ctgcttaacc cggtaacagg gttcacgaga ccgccattac caaaactgtt ctttcagtgt
     3301 agccgcagtc aggcctctac ttcaagacct cttaatctat cttctcgtgc actcctgtta
     3361 ctaatggctg ctgccagtgg cgttttgtcg tgtctttccg ggttggactc aagttgatag
     3421 ttaccggaca aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg
     3481 gagcgaactg ccttcacgga actgagtgtc aggcgtggaa tgagaaaccg cggccataac
     3541 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagctacca
     3601 gggggaaacg cctgggatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt
     3661 cagattttgc gatgcttgtc aggggggcgg agcctatgga aaaacggctt cgccgtggcc
     3721 ttatctcttc tttgctaagt gtcttcctgg tatcttccag gaaatttccg tccccgtccg
     3781 taagccggta ccgctcgccg cagccgaacg accgagcgta gcgagtcagt gagcgaggaa
     3841 gcggaatatc tcctgtatcg catattctgc tgacgcgccg gtgctgcctt ttttctcctg
     3901 ccacatgaag cacttcactg attttcgcaa tcgtggcaac atagtaagcc agtatacact
     3961 ccgctagcgc tacatgactg gttcagggct gcgccccgaa acccgctaac aacctctcag
     4021 ctactggata tcaccccatg ccatctgaat atgatgaaca actggattcg actgatacgc
     4081 agcttcccgg cgaatatgac ctttttgatt taatggcgca ggttactgaa gagaacctgc
     4141 atgcccctgt ggattttggg tcgcctgtcg gccgggaagc gttatagctt tttcgcttgc
     4201 ccgcgtacgt ggtaatactc cggtattacc ttttatttct ttattttcat gtaattacag
     4261 tggattttta atacccgggt attactcgtg aaaacgaaat attccttttt tttcttttaa
     4321 ttcatgcagt tgcgatgaaa atcgcggctg cgttaggtgt atgaccattt tgaggttgaa
     4381 aaataatcaa ccatggcggg tgatttacgc aggttaaggg gagatcgtga caaaaaaagc
     4441 gttcctcact gtatcgttca tgcctgcatg aacgggttat tcttcctgac tacagaatta
     4501 catcgctcct tcagttcata catcactttt acatcactta tacatcactt ttacatcact
     4561 tatacatcac ttttacatca ttccttcagc tcatacgtca ctttcatcct ctgtgccatt
     4621 tgtacatcac ccccttcctg aacgtgctga cagcgggttt tccggctgcg ccggcaacgc
     4681 ttcacggcca cgcaataaat tgcgtccccg ttcaccgttt tcggatggga agaacgccgg
     4741 aaacaggact taccccggct gaatggtgtg aatttccgcg ctatgcactt gtgcgcatac
     4801 tcatgcatgc cgtaaaaaca gagcctgcgc gtttctggcg ggttttcggg tggtttgttg
     4861 cctgttttac cggtttcccg tcagaaacgc cctgagggcc tctcaggcgg tgctgataac
     4921 agatgttatg gtaaatcaga gtcacctgca ctgttactct gaaccggagc gaaacagccg
     4981 cgaccagaat ccccgctttt tttcctgtgc cagcgcgtca gccagacggc taatttcggc
     5041 ctgtagttgt tccctctcct catttcgcag gcggttttct gcttcccgcg ccatcttgtc
     5101 ttcaagcatt agggtaacag tctgctgcag agcgtccagt ttctgctgaa tgacgtctag
     5161 ctgagcattt tccgcctggc taacgacaga tttggagtgt ccctccttgt gcctttttgg
     5221 tgtctcagaa gtgcgcagct cgccataggc ccgaatcagt tcactggtct cgaactcccg
     5281 gcgatcattt tttcctgtgc gatagctgac aaggccttta gccatgtcac gatataattg
     5341 cgagcgcgac tttcctgtta atttcagtgt ctcttgaact gtgtgccatg ccataaaagt
     5401 gacagtgtct cctgtgtgta ccgtattgta acagcagaac atattactgt tttaacacaa
     5461 aaggtctgag gctgcccgtg aggcgtgaaa aatacacaaa aaacctgttg acggtgtggt
     5521 tttgacgggc gtagtatttc gaatactgta tataaacaca ttgttataaa tacagttttt
     5581 gctgttttta aaagtcaaag aggataattt tatgagtaca gagttaaata gcggtactgt
     5641 cggtggtttt ggtcacatgg gaccaaatga cccctacaat gatggtcata gcggctctgg
     5701 aagttcggga ggtagtttta acggtggtgg taatggtggc catgataata gtagtggttc
     5761 atcttatact gcatcctttg gtctcatcac tggccgacac ccgataactg tcccaggtac
     5821 gaagaatatt aattctgtcg atctttctgc tgtcattaat aacattatac atcatgaagg
     5881 tgcacgcaat aacatgtacc tagatactgc gaacaatgta agagtgggag tgacgggcac
     5941 tggctggcaa tgtctagcaa cggcaggcat ttcggctgag ggtaaaagaa ctttccgcta
     6001 agcgatagac tgtatgtaaa cacagtattg caaggacgcg gaacatgcct catgtggcgg
     6061 ccaggacggc cagccgggat cgggatactg gtcgttacca gagccaccga cccgagcaaa
     6121 cccttctcta tcagatcgtt gacgagtatt acccggcatt cgctgcgctt atggcagagc
     6181 agggaaagga attgccgggc tatgtgcaac gggaatttga agaatttctc caaagcccgc
     6241 gacgcagcgc cggcaggcag agcaagtaga gggcagcgcc tgcaatccat gcccacccgt
     6301 tccacgttgt tatagaagcc gcatagatcg ccgtgaagag gaggggtccg acgatcgagg
     6361 tcaggctggt gagcgccgcc agtgagcctt gcagctgccc ctgacgttcc tcatccacct
     6421 gcctggacaa cattgcttgc agcgccggca ttccgatgcc acccgaagca agcaggacca
     6481 tgatcgggaa cgccatccat ccccgtgtcg cgaaggcaag caggatgtag cctgtgccgt
     6541 cggcaatcat tccgagcatg agtgcccgcc tttcgccgag ccgggcggct acagggccgg
     6601 tgatcattgc ctgggcgagt gaatgcagaa tgccaaatgc ggcaagcgaa atgccgatcg
     6661 tggtcgcgtc ccagtgaaag cgatcctcgc cgaaaatgac ccaaagcgcg gccggcacct
     6721 gtccgacaag ttgcatgatg aagaagaccg ccatcagggc ggcgacgacg gtcatgcccc
     6781 gggcccaccg gaacgaagcg agcgggttga gagcctcccg gcgtaacggc cggcgttcgc
     6841 ctttgtgcga ctccggcaaa aggaaacagc ccgtcaggaa attgaggccg ttcaaggctg
     6901 ccgcggcgaa gaacggagcg tggggggaga aaccgcccat cagcccaccg agcacaggtc
     6961 ccgcgaccat cccgaacccg aaacaggcgc tcatgaagcc gaagtgccgc gcgcgctcat
     7021 cgccatcagt gatatcggca atataagcgc cggctaccgc cccagtcgcc ccggtgatgc
     7081 cggccacgat ccgcccgata tagagaaccc aaaggaaagg cgccgtcgcc atgatggcgt
     7141 agtcgacagc agcgccggcc agcgagacga gcaagaccgg ccgccgcccg aaacgatccg
     7201 acagcgcgcc cagcacaggt gcgcaggcaa attgcatcaa cgcatacagc gccagcagaa
     7261 tgccatagtg ggcggtgacg tcgttcgagt gaaccagatc gcgcaggagg cccggcagca
     7321 ccggcataat caggccgatg ccgacagcgt cgagcgcgac agtgctcaga attacgatca
     7381 ggggtctgtt gggtttcacg tctggcctcc ggaccagcct ccgctggtcc gattgaacgc
     7441 gcggattctt tatcactgat aagttggtgg acatattatg tttatcagtg ataaagtgtc
     7501 aagcatgaca aagttgcagc cgaatacagt gatccgtgcc gccctggacc tgttgaacga
     7561 ggtcggcgta gacggtctga cgacacgcaa actggcggaa cggttggggg ttcagcagcc
     7621 ggcgctttac tggcacttca ggaacaagcg ggcgctgctc gacgcactgg ccgaagccat
     7681 gctggcggag aatcatacgc attcggtgcc gagagccgac gacgactggc gctcatttct
     7741 gatcgggaat gcccgcagct tcaggcaggc gctgctcgcc taccgcgatg gcgcgcgcat
     7801 ccatgccggc acgcgaccgg gcgcaccgca gatggaaacg gccgacgcgc agcttcgctt
     7861 cctctgcgag gcgggttttt cggccgggga cgccgtcaat gcgctgatga caatcagcta
     7921 cttcactgtt ggggccgtgc ttgaggagca ggccggcgac agcgatgccg gcgagcgcgg
     7981 cggcaccgtt gaacaggctc cgctctcgcc gctgttgcgg gccgcgatag acgccttcga
     8041 cgaagccggt ccggacgcag cgttcgagca gggactcgcg gtgattgtcg atggattggc
     8101 gaaaaggagg ctcgttgtca ggaacgttga aggaccgaga aagggtgacg attgatcagg
     8161 accgctgccg gagcgcaacc cactcactac agcagagcca tgtagacaac atcccctccc
     8221 cctttccacc gcgtcagacg cccgtagcag cccgctacgg gctttttcat gccctgccct
     8281 agcgtccaag cctcacggcc gcgctcggcc tctctggcgg ccttctggcg ctcctg
//
LOCUS       DAJVRF010000158         8286 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJVRF010000158 DAJVRF010000000
VERSION     DAJVRF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8286
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.158"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            490..1761
                     /locus_tag="OQN13_003444"
     CDS             490..1761
                     /locus_tag="OQN13_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS1616715.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(1791..2795)
                     /gene="dppF"
                     /locus_tag="OQN13_003445"
     CDS             complement(1791..2795)
                     /gene="dppF"
                     /locus_tag="OQN13_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCS1616716.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(2792..3775)
                     /gene="dppD"
                     /locus_tag="OQN13_003446"
     CDS             complement(2792..3775)
                     /gene="dppD"
                     /locus_tag="OQN13_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCS1616717.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(3786..4688)
                     /gene="dppC"
                     /locus_tag="OQN13_003447"
     CDS             complement(3786..4688)
                     /gene="dppC"
                     /locus_tag="OQN13_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCS1616718.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(4698..5717)
                     /gene="dppB"
                     /locus_tag="OQN13_003448"
     CDS             complement(4698..5717)
                     /gene="dppB"
                     /locus_tag="OQN13_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCS1616719.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(6025..7632)
                     /gene="dppA"
                     /locus_tag="OQN13_003449"
     CDS             complement(6025..7632)
                     /gene="dppA"
                     /locus_tag="OQN13_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCS1616720.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(7984..>8286)
                     /locus_tag="OQN13_003450"
     CDS             complement(7984..>8286)
                     /locus_tag="OQN13_003450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616721.1"
                     /translation="VGLIRRASVASGNGTAFIVGCGATALFDQHTTHFPANFAPHISL
                     SINGFVTETIVFFYICLNLFLRYLWLALAFSAVRFSVHGINRSDIHLFLRFFSLNA"
BASE COUNT         1909 a         2245 c         2095 g         2037 t
ORIGIN      
        1 tctgcgagcg tcatatagcc gcctgtgttg taatgacaac gtttcgcggc tattcttgag
       61 tggtctaggt tcaagattag cccccgttat gttgtcaggt acatacctgc aacgtgcggg
      121 ggttttctct ctccagcaac caatgccacc agggataaag cccccgcaac attgcgcctc
      181 accggaaccc tttcggcttg ccgctgattt tacgattgct ttttactgtc agcacccggc
      241 aatcttcctc attgcgcgat gccttccagc ttaatttctt tatttgcaat agttgcagtt
      301 catatccggg ctgactccag actggtagtg aaatgcgcaa ttttgtagaa ttgcgcctcc
      361 tcgcgacctc cccagtgaat ctgttgcctc attttttaca tactgcgtat tcgacttctc
      421 cacctgttgc gcaagagaaa ctgggtttat tcatttttgc gaggccgact tctttctgga
      481 caggacttta tgcagcacaa cacactatcg aaacacaatc agaaattgcc gtttacacgc
      541 tacgacttcg gctgggtttt attatgcata ggcatggcga ttggtgccgg aaccgtgctg
      601 atgccagtac aaattggctt gaagggaatt tgggtattta ttaccgcagc gatcattgct
      661 tatcctgcca cctgggtagt gcaggacatt tatttaaaaa ccctttctga aagcgattcc
      721 tgtaatgact acaccgatat tatcagtcat tacctgggga agaactgggg aattttcctc
      781 ggggttatct actttttgat gattatccac gggattttta tctactctct ctccgtggtt
      841 ttcgacagcg cctcgtacct gaaaaccttc ggtttaaccg atgccgatct ttcacaatct
      901 ctactttata aagtcgctat tttcgccgta ctggtagcga ttgcgtctgg tggtgaacga
      961 ttactgttta agatttccgg gccaatggtg gtggtcaaag tagggattat tgtcgtgttc
     1021 ggttttgcga tgatcccgca ctggaatttc gccaatataa ccgccttccc gcaagcctcc
     1081 gtctttttcc gcgatgtctt gcttaccatt ccattttgct tcttttctgc agtatttatt
     1141 caggtactta acccaatgaa tattgcctat cgtaaacggg aagcggataa agtactggca
     1201 acccggctcg cgctgcgtac ccaccgaatt agttatatca cgctcatcgc ggtgatcctg
     1261 ttttttgcct tttcgtttac cttctcaatt agccacgaag aagccgtttc tgcctttgaa
     1321 caaaatatct cagcactggc gctggccgcg caggtgatcc ctgggcatat cattcatatc
     1381 acctctacgg tgcttaatat ctttgccgta ctgaccgcat tctttggcat ttatctcggt
     1441 ttccacgagg ccattaaagg cattattctc aatctgttaa gccgaattat tgataccaag
     1501 aaaattaact cacgcgtgct gactctggcg atctgcgctt ttatcgtcat tacgttgacg
     1561 atttgggttt cgtttcgtgt atcggtgctg gtgttctttc agttgggaag cccgttatat
     1621 ggtattgtgt cgtgcctcat tccgtttttc ctgatctata aagtcgcaca actggaaaaa
     1681 cttcgcggat ttaaagcctg gctgattctg ctgtacggca ttttgctatg cttgtcgcca
     1741 ctgttgaagc tgattgagta aaccggagcg catggccccg gttttgtgag ttaacgctgc
     1801 ggattttcat cctgatcaac agcaaaacaa gctaccagtt gaccgccgta gtcttttagc
     1861 tgcggctgca actgggtgca ggggccgaag cgccgacgac agcgggcgtt gaaggcgcaa
     1921 cccggcggtg gattcagtgg gcttggtagt tcaccgctga gcttgatgcg ctcgcggcga
     1981 tcgtccgggt tcaggcgcgg cgtcgcggaa agtagcgcct gagtgtacgg atggcgcggg
     2041 ttattgaaga tttggtcttt cgttcccttc tccacgcagc ggcccaggta catcaccatc
     2101 acttcatcag caatgtgctc caccaccgac aggtcgtggg agataaagac ataagacagc
     2161 cccaactcct gctgcaaatc catcatcaga ttcagcacct gcgcgcgcac tgaaacatcc
     2221 agcgcggaaa ccggttcatc ggcaatcacc acatccgggt cgagcatcag accacgggcg
     2281 atggcgatac gctgacgctg accgccggag aacatatgcg gatagcggtc atagtgctcg
     2341 gttttcaggc cgactttcgc catcatcgac agggcttttt cccgacgctg ttctttgctt
     2401 aagctggtgt tgatcagcag cggctcttca agaatttgcc cgactttttt acgcggattc
     2461 agcgaaccgt aagggttctg gaagacgatc tggattttct gccgacgcag cttctgcgcc
     2521 tgcggatcgt gcttaagcag atcctgcccc tggtaataca gctcgccacc ggtgggcgtt
     2581 tcaatcatcg tcagcaaccg accgagggtc gatttaccgc agccagattc gcccactact
     2641 gccagcgttt tgccacgttc aaggttaaac gaaacgccat ccagcgcttt aaccagacgt
     2701 tccggcgcga acatgccttt cttcaccgga taatgttttt tcaggtcgat agcctgcaac
     2761 agcggttgtt gcagggtggc ctcttgcgta ctcatagtgt cggcctcccg gcatcatcaa
     2821 gtgggtaatg gcatttggac tggcgcccgt cagcgagcat attcagcgcc ggttcttcag
     2881 cgcgacatct gtcagtggca taggggcagc gcgggttaag caggcagccg ttcgggcggt
     2941 cgtacttgcc gggaacgaca cctggcaacg acgccagacg ttctttgtcc tgagcaaatt
     3001 ctggcagcgc acgcagcaat gcctgagtat acgggtgacg cggcgcatgg aagatggcgt
     3061 gcgcatcgcc cgtttccacc acctggcctg catacatcac aatgatttta tgcgcagctt
     3121 ccgccaccag cgccagatca tgggtaatta acaccagcgc catgttctct ttctgctgta
     3181 gctccagcaa cagttcgatg atttgcgcct gaatggtcac gtccagcgca gtggtcggct
     3241 catcggcaat cagcagtttt ggccgacagg caatcgccat ggcgatcatc acgcgctggc
     3301 tcatgccgcc ggaaagctga tgcgggtaaa catccagacg cgatgccgga tcgggaatac
     3361 cgacctgatt cagcaggtca atcgcccgct gacggcgagt gctcttatta ccgccctgat
     3421 gcaccttaat cgcttccatg atctggaaac ccacggtgta gcacgggtta aggctggtca
     3481 tcgggtcctg gaagatcatc gccacttcgg cacccaccag gttgcggcgc tctttttctg
     3541 atatacgctg caaatcctga ccgttaaaca ccagcttttc cgccattacg cggcccggat
     3601 aatcaatcag ccccataatc gccagtgaac tgaccgactt accggagcca gactcaccca
     3661 caatcccgac cacttcgccc tgttttacgc tgtagctgat gcggtctacg gcgcggaacg
     3721 gtgcgctttc gtcgccgaaa tgcaccgata atttatctac atttaataac gccatctcga
     3781 acctcttact gcttcagttt gggatcgagc gcgtcacgca gaccgtcacc catcaggtta
     3841 aatgccagca ccgtcagcag gatcgccaga cccgggaagg tcacgaccca ccaggcgctt
     3901 tgcgcgaact gcaacacgtc ggagagcatg gtgccccact ctggtgttgg cggctgcgct
     3961 cccatgccga ggaagccaag ggcggccata tcgagaatgg cgttagagaa accgagcgac
     4021 gcctgaacaa tcagcggcgc caggcagttc gggaagatgt taataaacat ctgacgcatc
     4081 gccccggcac ctgccacgcg agacgcggta acgtaatcgc gattcacctc caccagcacg
     4141 gcggcgcggg ttaagcgcac atagtgcggc aaggcaacga aggtcagtgc cagcgcggcg
     4201 ttaccaatcg acgggccgaa aatcgccacc agcaccagtg ccaacagcag acttggcagc
     4261 gccagcatga tatcgaccac gcgcatgatg atgttatcga ccaggccgcc gaagtaaccg
     4321 gcgatcaggc cgagaataac gcccataatc agcgataaca ccaccaccag acagccaacc
     4381 agcagcgaca agcgcgcgcc gtacatcagc cgcgacagca catcacggcc tacgtcatcg
     4441 gtgcccagca agtgcgccat gctgccgcct tcctgccagg ctggcggggc gagcagcgca
     4501 tcgcggaact gatccgccgg gttataaggc gcaatccagt tggcaaagat cgtgataaac
     4561 agcacgatga cgatgtaaac cagcccgaca accgcgcctt tgttgcgttt aaaatagtgc
     4621 cagaactcct gtaacggggt catcggcacc ggtgcgctaa tcactttatt ttcagtaacc
     4681 tgtgacatga tgtcccctta cttcttatga cgaatacgcg ggttcaccac gccgtacagc
     4741 agatcgacca gcaggttgac gaggataatc atcgtcgcca ccagcaatac gccgccctgc
     4801 actaccggat agtcgcggcg ttgcagtgcg tcaatcaacc agcgtcccag accgggccac
     4861 gagaagatgg tttcggtcag aatcgccccc gccagcaatg ttcccacctg caggccgata
     4921 acggtcacta ccggcagcat cgcgttacgc agcgcatgga cgataatcac ccgcatgcgg
     4981 gtcaaccctt tggcgcgcgc ggtgcggatg taatcttcac ccagcacttc cagcatcgaa
     5041 gagcgcgtca tacgaacgat aaccgccagc gggatagtac ccagcacaat agccggtaag
     5101 atcatatggg caacggcgtc gataaagttg ccatcttcac cccagatggc ggtgtcgatc
     5161 agcataaaac cggttaacgg attggagtca tcgaggaaca ccatatcgct cacgcgaccg
     5221 gagacgggcg tcaggttcca gtgcaccgaa accagcatga tcagcatcat gccccaccag
     5281 aagatcggca tcgagtagcc ggtcagcgcc aggccaactg cagtgtgatc gaaaatggaa
     5341 ccgcgtttaa ccgcagccag cacaccaacc ggaataccga ctgccgtagc aaaaatcatc
     5401 gcgcagacgc caagttccag cgtggcctga aagcgcggca cgaactcttc ccatactgga
     5461 attcggcttt tcattgaaat gcctagatcg ccgtgcatta cgccccaaat gtaatggaga
     5521 tactgctgcc acatcggttt atctaagccg agttcagcca gcagctgcgc gtgacgctca
     5581 ggggagatcc cgcgttcgcc tgccatgatc atcaccggat cgcccgggat catgtggaca
     5641 aaggcaaatg tgagaagggt aataccgata aacgtgggga tgacgagtcc caaacgtcgg
     5701 agaataaact gcaacataac ccggattctc tatagtgacc cgcagcctgg aactgcgtgt
     5761 ctgtattgct cacaaatcaa ttcccgccct tatcggacag ggaatgaagt attgctgccg
     5821 gatggcgctg cacgcagagg cacatcgcca ccgggtatac agattgttga caaagtgcgc
     5881 tttgtttatg ccggatgcgg cgttaacgcc ttatccggcc tacaaaatcg tgcaaattca
     5941 atacattgca gaattttcgt aggcctgata agcggagcac atcaggcagt tttgcctttg
     6001 ccatcagtct tgtatggctt ttaattattc gatagagacg ttttcgaagt gatgtttgcc
     6061 taatggatca accacatagc ctttgacttc tttgcgtacc ggttcaaaca cggtggagtg
     6121 agcgatgatc agtgccggag tctgatcgtg catcaccacc tgcgcttgct tgtacagttc
     6181 aacgcgtttg ttatggtcgt cggtagcacg cgccggttga atcagatctt caaacggttt
     6241 gtagcaccat tttgagtagt tagaaccttg ttcagaggcg gcgcagctga acagggtggc
     6301 gaagaagtta tccgggtccc cgttatcgcc agtccagccc atcattaccg tctgatgttc
     6361 gccatctttc gcacgcttga ggtactcacc ccattcgtag gtgacgattt tggcctgcac
     6421 gcccactttc gcccagtctg cctgaatcat ctccgccatg cggcgagcgt tcgggttata
     6481 cggacgttgt accggcatcg cccacaggtc gatggagaaa cctttttcca gacccgcttc
     6541 tttcagcaag gctttcgctt tttcaggatc gtaggtgtaa tcctgaacgt cgtcgttata
     6601 gccccacatg gttggcggga tcaggttttt cgctgataca cccgcgccct gataaaccgc
     6661 tttgatgatc gcgtctttgt tcaccgcgta ggtcagtgcc tggcgaactt tcacgtcatc
     6721 cagcggtttt ttctgcacgt tataggagag gtagccgacg ttcagccccg gcatttccat
     6781 caggttgatg gatttatcct gcttcatgcg agcgatgtct gccgggttcg ggtacggcat
     6841 cacctggcat tcattcttct gcaatttcgc gtaacgcacg gaagcatcag gggtaataga
     6901 gaaaaccagc gtatcgatct gcggtttggt gccccagtag ccatcaaacg ctttgtagcg
     6961 gatacgggaa tctttttggt actgctgtaa ctggaacgga ccggttccga ttgggttgag
     7021 gtccagtttt tccggtgtac cggctttcat catcgcatca gcatattctt ttgacagaat
     7081 agaggcgaag tccattgcca gatcagcgag gaacggcgct tccgggcgag tcagtacgaa
     7141 ctgaactgtg ttgtcgtcca cctttttcac ttcactgatc agctccggca agcccatgcc
     7201 ttcgaagtat tcgtagctgc cgccagagac tttatggtac gggttttgcg cgtttttctg
     7261 acggtcgaac gagaacacca cgtcatcggc gttcagttca cgcgtcggtt tgaattcttt
     7321 attgtcgtgc cacttcacac ctttacgcag atggaaggta taggttttac cgtcttcgct
     7381 gacttcccac ttttcagcga ggcccgggat cacttcagtg gtgccgattt taaattcaac
     7441 caggcggtta taaagcggta cggaagaggc gtcataggtg gtgccggagg taaacagctg
     7501 cgggttaaac ccttccggag atccttctga gcaataaacc agagttttag cctgaacact
     7561 tgctgcgacg gtcatagcca ccaggctgag accaagcttc agcatccctg actttttcaa
     7621 ggaaatacgc attattctgc tccaattgtg atgtttgttg ttttaaccct ttgcagtggg
     7681 ttgtcgctgc ctgacctttt ttgttttttg cccggtcggg tcaacgttat gaggtgggga
     7741 tgccgtacta attaacatca gtgtaacagt gcggatcctc cataaaatgc ccctcgtgac
     7801 ctacaatctg tcaacagaat gtgaaaacgt caatacagcc aaccgggatt tacaccaacg
     7861 gtgagaatcc acaaacaaag attaaaaaaa cttcaaacag ctatttgcag cagagaaatt
     7921 tgtgctactc caacataacc agaacaatca gcttaatatt tagcaacatt tggtgattaa
     7981 gttttatgca tttaatgaaa aaaatctgag gaaaaggtgg atatctgagc gattaatacc
     8041 atgaacgcta aaacgcacag cggaaaatgc cagcgccagc cataagtaac gcaaaaaaag
     8101 atttaagcaa atataaaaaa agacaatggt ttctgtcaca aatccgttaa tggatagtga
     8161 gatatggggc gcaaagttgg caggaaaatg tgtggtgtgt tggtcgaata aggcggtcgc
     8221 gccgcatccg acaataaatg cggtgccgtt gcctgatgcg acgctggcgc gtcttatcag
     8281 gcctac
//
LOCUS       DAJVRF010000159         8077 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJVRF010000159 DAJVRF010000000
VERSION     DAJVRF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8077
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.159"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..736
                     /locus_tag="OQN13_003451"
     CDS             <1..736
                     /locus_tag="OQN13_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCS1616722.1"
                     /translation="KPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPA
                     HGFARNLPWTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLE
                     SHGEFETTSALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRV
                     YLNPQDCSVINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVET
                     AYASETQKVTKEKPAHLAQSIRVAKR"
     gene            complement(787..1641)
                     /gene="yeaE"
                     /locus_tag="OQN13_003452"
     CDS             complement(787..1641)
                     /gene="yeaE"
                     /locus_tag="OQN13_003452"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCS1616723.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1731..2477)
                     /gene="mipA"
                     /locus_tag="OQN13_003453"
     CDS             complement(1731..2477)
                     /gene="mipA"
                     /locus_tag="OQN13_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCS1616724.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLTGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2913..4847
                     /gene="yeaG"
                     /locus_tag="OQN13_003454"
     CDS             2913..4847
                     /gene="yeaG"
                     /locus_tag="OQN13_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCS1616725.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            4960..6247
                     /locus_tag="OQN13_003455"
                     /pseudo
     CDS             4960..6247
                     /locus_tag="OQN13_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
     gene            6394..7869
                     /locus_tag="OQN13_003456"
     CDS             6394..7869
                     /locus_tag="OQN13_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCS1616726.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT         2146 a         1928 c         1879 g         2124 t
ORIGIN      
        1 gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac aacacaccgt tcaaaaatgg
       61 cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg tttggtccgg cggcacaaca
      121 aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg acgctgaaat cacataatga
      181 agatgctgat ggcgtagcgc tgacttttga attgacgcaa agcgaagaga cgaaaaaatt
      241 ctgtccgcac gactttacgc tgttagcgca tttccgcgtg ggtaaaacct gtgaaatcga
      301 tcttgaatca catggcgaat ttgaaaccac ctctgccctg catacctact ttaacgtggg
      361 tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc ttcattgata aagtgaatga
      421 cgcgaaagaa gatgtactga ccgacggtat tcagaccttc cctgaccgta ccgatcgcgt
      481 gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa gcgctgaatc gtattatcgc
      541 cgtaggccac cagcatcatc tgaacgttgt aggctggaac ccgggaccgg cgctttcaat
      601 tagcatgggc gatatgccgg atgatggcta caaaacattt gtttgtgtag aaacggctta
      661 cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca catctggcgc aatccattcg
      721 cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg tgcgatgcat ccggcacgta
      781 tcaacatcac accatatcca gcgcagtttt tccttttggt gccggatacg ctttatccag
      841 catggttaat tccgctgacg aaagttcgac ctcaagcaca gccgcattct gttgaacatg
      901 ggcaatcgtg gccgcttttg gaatcgccat cacaccctga tggctgatta cccacgccaa
      961 caatacttgt gccgagctga tattgtgagc atgtgcaatt tcgttgacta ccgcgttttt
     1021 taacagtcca ttgcgcaacc gcccggcctg ggctaatgga ctgtaagcca tcaccggcat
     1081 ctgctgttgc tggcaccagg ggagtagatc gtactcaatc cctcgtgaac cgagatggta
     1141 aagcacctga ttagtggcac actgatttcc ccccggcagc tgccagagtt cctgcatatc
     1201 agcataatca aggttagaaa cgccccagcg gcggattttt ccctgggcga tcaatttttc
     1261 catcgctgca acagtctctt caaaagcgaa actgccagac ctgtgtaata agtagagatc
     1321 gagataatca gtattgagac ggcgtaaact ggcttcgcat gcatttatcg ctttttgccc
     1381 gccagcattc cacggataga ctttagagac gagaaagacg ttatcacgca gaccggttaa
     1441 tgcttcccca accacctttt cggcaccgcc atcggcatac atttcggcgg tatcaatgag
     1501 ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact tctgttttgc gctgactggc
     1561 atcttcgccc atataccatg ttccctgccc tatggctggc agtgagacat cgccactaaa
     1621 ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg caccaccgta atgcaaaaca
     1681 gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa agatgcatta tcagaatttg
     1741 taggtgatcc cggtagaaat caggccagtc caggatttat ccaccatcgg gctgtcagta
     1801 acttcatcag acagacgggt atagcgcgcg gtaccgtaaa cactccagtc gccgaggaag
     1861 ttgtagctgg cgctcagctc caggtaaggg ctccagctgt cgttcgggtt atagccacgc
     1921 agaccgctgc gagcggactc tttgcgcgat acgccataat agtattcgtt ctggttttcg
     1981 ctgttccact gcacaccgat acccggggtc actgtcaggc cgccgttggt gtaacgatac
     2041 aaccaggcca tatcccagac aatgccgttg ctgttatcca gggtatcgcc agtcagggtg
     2101 gtacgcaggt aaccgtactg ggtaaagtga gcataagaca gaccagccat catggtgctc
     2161 ttacggtcat ccaggtgacg catttggtga tcgccactgt ctttcgcttt gaagtaaagc
     2221 ggcgaccaat aagcggtaat tgaaagtttg tccgttgcgt cattccacag gtagtaacca
     2281 ccacctaagc cacggaacca gaagttatcg ccttcatagt tgattaccgg tactgggtaa
     2341 acatcggtat cgtaatcttt atatgggtgt tcaacgacac cgacgcctgc gcccagggaa
     2401 aatttacctt cagcgtgcgc tacgcctgca gacgttgcga taagcactcc aagtgccaga
     2461 agtttgagtt tggtcacaat taatcattcc ttaaacaaat gtttagcggg cgacaaagtt
     2521 tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc attttttaaa gtaactgttt
     2581 aattttcctg acgcggatga caccgcgctt acagccaaat gaatttagcg ttactggcga
     2641 gccttgtctt tacattaatt atgcaaaatt tatggatgag ttgttgatat gccattgaaa
     2701 ttaagaaagc cgtgcaggca agttttccat ttgccatcta cgcttaattt tgaaggtgta
     2761 tcaccgggca cgttgttctc atcgtcgata aaatggcatg agagttgctg tgttttagca
     2821 agagacgtcg ttcagtttac ctcttccggg agcctctact attcatatga acggctctta
     2881 acctgtgcta aaaaacgaaa ggacggcata ccatgaatat attcgatcac tatcgccagc
     2941 gatatgaagc tgccaaggac gaagagttca cactgcagga gtttcttacc acttgtcggc
     3001 aagatcgcag tgcttatgcc aacgcggctg agcggctatt gatggctatc ggtgagcctg
     3061 tcatggtcga tacagcccag gaacccagac tttctcgact cttttctaac cgggtcattg
     3121 cacgttatcc ggcatttgaa gagttttacg gcatggaaga cgcgattgaa cagattgtct
     3181 cttatctgaa acacgcggct caggggctgg aagagaagaa acaaatcctg tatctgctgg
     3241 ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact gaaatcatta atgcagctcg
     3301 taccgattta tgtattgagc gccaacggtg agcgtagccc ggtcaacgat catccgttct
     3361 gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa agagtatggc attcctcgcc
     3421 gctatctcgg caccatcatg tcgccgtggg cggcaaaacg cctgcatgaa tttggtggcg
     3481 atatcactaa gttccgggta gtgaaggtct ggccgtcaat tctgcaacaa attgctatcg
     3541 ccaaaacaga acccggtgat gagaacaacc aggacatctc cgcgctggta gggaaagtcg
     3601 atattcgtaa actcgaacac tacgcgcaga atgacccgga cgcctacggc tattccggtg
     3661 cgctgtgccg cgccaatcag gggatcatgg aatttgtcga gatgtttaaa gcaccgatta
     3721 aagtgctgca tccgctgtta accgctactc aggaaggtaa ctacaacggg acggaaggta
     3781 tctccgccct gccgttcaac gggattattc tcgcccactc gaacgaatcc gaatgggtca
     3841 ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg tgtttacatc gtgaaggtgc
     3901 cgtattgctt acgcatttcc gaagagatca aaatctacga gaaattgctt aatcacagtg
     3961 aattgactca cgccccatgc gcccctggca cgctggaaac actgtcacgt ttttccattc
     4021 tttcgcgcct gaaagagcca gaaaactcca gcatttattc aaagatgcgg gtttatgatg
     4081 gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta tcaggaatat cgtgactacg
     4141 ccggtgtcga tgaagggatg aaaggtctgt cgacgcgttt tgcgtttaag atcctctccc
     4201 gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc ggtccatctg ttctacgtcc
     4261 tagaacagca gatcgagcgc gagcagttcc cgcaagagca ggcagaacgc tatctggagt
     4321 tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat cggcaaagag atccagacgg
     4381 cctaccttga atcctattcc gaatatgggc aaaacatttt cgaccgttat gttacctacg
     4441 cggatttctg gattcaggat caggagtatc gcgatccgga taccgggcag ctgtttgacc
     4501 gcgagtctct taacgccgag ctggagaaaa tcgagaaacc ggcggggatc agtaatccaa
     4561 aagatttccg caacgagatt gttaacttcg tactgcgcgc cagagcgaat aacagcggac
     4621 gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt tatcgagaag aaaatgttct
     4681 ccaataccga ggagctgttg ccggttatct cgtttaacgc caaaacgtca accgacgagc
     4741 agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa aggctacacc cgtaaacagg
     4801 tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc gtcttaacaa ccctggcccg
     4861 gtacacatgc taccgggcct ataacgacag cgaaccgtgg gccagagaaa tggcaacaca
     4921 ggcgtagcat acagttggca aatgtagtac ggggggcata tgaccgggtt tattgaccgg
     4981 cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc gttttttacg ccgttataaa
     5041 gcgcaaatta aacagtcgat ctccgaggcc attaataagc gttcggtgac tgacgtcgac
     5101 agcggcgaat ccgtatccat tcccacggaa gatattagcg aaccgatgtt tcatcagggg
     5161 cgtggcggcc tgcgccaccg cgtacatccg ggcaatgacc atttcgtcca gaacgaccga
     5221 attgaacgtc cccagggtgg cggcggaggt tccggcagtg gtcagggcca ggccagccag
     5281 ccaggatggt gaaggtcagg atgaatttgt ctttcagatt tcgaaagatg agtatcttga
     5341 tctgctcttt gaagatttgg ccttaccgaa tctgaaacaa aaccaacaac gccagctgac
     5401 cgaatataaa acgcatcggg cgggttatac cgctaacggc gttccagcca atatcagcgt
     5461 tgtgcgttcc ttgcagaact cactggcgcg acgcacagcc atgacggcgg gcaagcgacg
     5521 ggaacttcat gcgctggaag agaatttggc catcatcagc aacagtgaac ctgcgcaact
     5581 gctggaagag gaacgtctgc gcaaagaaat tgcagaatta cgtgccaaaa ttgaacgcgt
     5641 cccttttatt gacaccttcg atttacgtta caagaactac gagaagcggc ccgatccctc
     5701 cagccaggca gtgatgtttt gcctgatgga cgtttccggt tcaatggatc aatccactaa
     5761 agatatggct aagcgttttt atattctgct gtatctgttc ctcagcagaa cgtataagaa
     5821 cgtggaagtc gtatacatcc gccatcatac ccaggcgaaa gaagtcgatg aacatgagtt
     5881 tttctactcg caggaaacag gcggcaccat tgtttccagc gccctgaaac tgatggatga
     5941 ggtagtgaaa gagcgttata acccggcaca gtggaatatt tacgctgcac aagcatcgga
     6001 cggcgataac tgggccgatg actctccgct ttgccatgaa atcctggcga aaaaattatt
     6061 acctgttgtt cgttattaca gctatatcga aattacccgt cgtgcacatc agacattgtg
     6121 gcgagaatat gagcatctgc aatctacttt cgacaacttt gcgatgcagc acatccgcga
     6181 ccaggatgat atttatccgg tgttccgtga actgtttcat aaacaaaatg caacagctaa
     6241 agactaaaac tatcagccag gtcattatcg cctggctgat ttttagctta ctgtaaatta
     6301 tctcatttat tacatacatt agcttacaat cgctttaaat atgacagcat aacctttaca
     6361 taatttagtt ccagaaaaca atcattcgga aaaatgattc agtcaacacg tatttccatg
     6421 gggttattct ttaaatattt tttatcgtta acgaaaattg atcctggtca aaactatata
     6481 tctctgccat caataaaatc cagcactcac attgctctcc tttttatggt ttctatgggt
     6541 acacaaaaat taaaagctca aagctttttt attttcagtt tattgctgac gttaatttta
     6601 ttttgcatta ctaccttata taacgaaaac acaaatgtaa aactcatccc acagatgaat
     6661 tacctgatgg ttgttgtggc tttgtttttc cttaacgccg tcatttttct tttcatgtta
     6721 atgaaatatt tcactaacaa acaaatttta ccaacactca ttttaagcct tgcattttta
     6781 agtggcctta tctatttagt tgaaaccatt gtaattatcc ataaaccaat taacggcagt
     6841 acactgatcc agacaaagtc gaatgatgtt tctattttct atattttccg ccaactcagt
     6901 tttatttgtt taacctcgct ggcactcttt tgttatggaa aagacaacat ccttgacaac
     6961 aataagaaaa aaacgggaat cctgttgctg gcgctgattc cttttttagt ttttcccctt
     7021 ctggcacaca atctgagcag ttataacgct gactattctt tgtatgtcgt cgactactgt
     7081 ccggacaacc atactgcgac ctggggaatc aactatacaa aaatattggt ttgtctgtgg
     7141 gcatttttac tgttctttat tatcatgcgt acacgattag ccagcgaact atggccgtta
     7201 atagcattat tatgtctggc atcgctatgc tgcaacttac ttctactgac tctggatgag
     7261 tataattaca ccatctggta tatcagtcgc gggattgaag tttccagtaa actgtttgtt
     7321 gtgtcttttc tgatttataa catttttcag aaactgcaac tctccagcaa actggcagtt
     7381 catgatgtgc tgaccaatat ttataatcgg cgctactttt tcaacagcgt agagtcatta
     7441 ttgtcgagac ctgttgttaa ggacttctgt gtcatgctgg ttgatattaa tcagttcaaa
     7501 cgcatcaatg cccaatgggg acatcgtgtg ggtgataaag tgctggtttc aattgtcgat
     7561 attatccagc aaagcatccg ccccgatgat attttagcgc gactggaggg tgaggtgttt
     7621 ggcttgctat ttaccgaact caatagtgcc caggcaaaaa tcattgcgga acgtatgcgt
     7681 aaaaatgtcg aactcctgac cggctttagt aacagatatg atgttcctga acaaatgacc
     7741 atcagtattg gcacggtttt ttcaacgggt gacacgcgta atatctcgct tgtcatgacg
     7801 gaagcagata aagccttacg cgaagcgaaa agcgaggggg gcaacaaagt gattattcat
     7861 catatttaag tgcaaaaata ttcagagcca tgctttttgc gtggcttttg catacaattt
     7921 attactatta cccctaaatt tcccctcccc agcggcgcag agatgagtat aattagcgcc
     7981 cctgtgccag gccgcaatcg aactttatct ggttttctcg tttcactgaa ccgaaggagt
     8041 gccatttatc atgaaattgc accatagaat gctccgg
//
LOCUS       DAJVRF010000160         7947 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJVRF010000160 DAJVRF010000000
VERSION     DAJVRF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7947
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.160"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..360
                     /locus_tag="OQN13_003457"
     CDS             <1..360
                     /locus_tag="OQN13_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HCS1616727.1"
                     /translation="TGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLG
                     ERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFLL
                     RKDIMRRLTQDVCEEIE"
     gene            complement(417..2795)
                     /gene="ppsA"
                     /locus_tag="OQN13_003458"
     CDS             complement(417..2795)
                     /gene="ppsA"
                     /locus_tag="OQN13_003458"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCS1616728.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            3128..3961
                     /gene="ppsR"
                     /locus_tag="OQN13_003459"
     CDS             3128..3961
                     /gene="ppsR"
                     /locus_tag="OQN13_003459"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCS1616729.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4118..5164
                     /gene="aroH"
                     /locus_tag="OQN13_003460"
     CDS             4118..5164
                     /gene="aroH"
                     /locus_tag="OQN13_003460"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCS1616730.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            5296..5487
                     /gene="hemP"
                     /locus_tag="OQN13_003461"
     CDS             5296..5487
                     /gene="hemP"
                     /locus_tag="OQN13_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCS1616731.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5491..6927)
                     /locus_tag="OQN13_003462"
     CDS             complement(5491..6927)
                     /locus_tag="OQN13_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCS1616732.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(6990..7703)
                     /gene="ydiV"
                     /locus_tag="OQN13_003463"
     CDS             complement(6990..7703)
                     /gene="ydiV"
                     /locus_tag="OQN13_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCS1616733.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         2031 a         2086 c         1888 g         1942 t
ORIGIN      
        1 accggacgca aaaaagatat tattgtccgc ggcggcgaaa atattagcag ccgtgaagta
       61 gaagatattt tattgcagca tcctaaaatt cacgatgcct gtgtggttgc aatgcccgat
      121 gaacgtttag gtgaacgatc atgcgcttat gtcgtgctga aagcgccgca tcattcatta
      181 tcgctggaag aggtggtggc tttttttagc cgtaaacggg tcgcaaaata taaatatcct
      241 gaacatatcg tggtaatcga aaaactaccg cgtactgcct caggtaaaat acaaaagttt
      301 ttgttaagaa aagatattat gcggcggtta actcaggatg tctgtgaaga gattgaataa
      361 gtttcatctt cggggatcac ataaccccgg cgactgaacg ccgccgggga tttattttat
      421 ttcttcagtt cagccaagct taaccaggtt tgcaccacgg tgtccgggtt cagagacagg
      481 ctatcgatcc cctcttccat caaccatgcg gcaaagtctt cgtggtcgga cggaccctga
      541 ccgcaaatcc cgacatattt gccctgtttc ttcgcggcac ggatagccat cgacagcagt
      601 gctttcaccg catcgttgcg ctcatcgaac aattcagaca ccacgccgga gtcacggtcc
      661 agaccgagcg ccagctgcgt catatcgttt gagccaattg agaagccgtc gaaatattcg
      721 aggaactgct cggccagcaa ggcgttggac gggatttcac acatcatgat gattttcagc
      781 ccgttctcac cacgcttcag cccctgacgc gccagttcct caaccaccgc tttcgcctga
      841 tctacggtac gcacgaacgg gatcatgatc tcaacgttgg tcagccccat gtcgttgcgc
      901 acacgtttca ctgcttcgca ctccagcgcg aagcagtcgc ggaagctgtc ggaaacatag
      961 cgtcccgcgc cacggaagcc gagcatcggg ttctcttcat ctggctcgta acgctcacca
     1021 ccgaccaggt tggcatattc gttcgattta aaatcagaga gacggacaat gacgcgcttc
     1081 ggataaaacg cggcacccag cgtcgcgatc ccttcagtca gacgaccaac gtaaaattca
     1141 cgcggagaat caaaaccttt catcatctcg cggatttcat tttgcaactg cggttcctga
     1201 tcgtcaaact caagcagtgc gcgtgggtgg acgccaatca tacggttgat gataaattcc
     1261 agacgcgcaa ggcccacgcc ttcgttcggc aggcaggcga agtcgaaagc acggtccggg
     1321 ttaccgacgt tcatcatcac tttcaacggc agatccggca tcatttctac gctggagctt
     1381 ttcacgctaa attccagcaa ctccgcatag acgtaaccgg tatcaccttc ggcacaagaa
     1441 acagtgacgt tctcaccgtc tttcatccgt tctgttgcat caccacagcc cactaccgcc
     1501 ggaatgccca gttcacgagc gatgatcgcc gcgtgacagg tacgaccgcc acggttggtg
     1561 acgatggcag atgctttctt catgatcggt tcccagtccg ggtcggtcat gtcagtgacc
     1621 agcacgtcac caggttcgat gcggttcatt tcgctgatct catggatgac tttcaccgga
     1681 cccgcgccga tgcgatgacc gatagcacgg ccttcggcga taatcttacc ctgtgaatgc
     1741 agcgtataac gctccatgac ctgaccgcgt gagcgcacgg tttccggacg cgcctgcaca
     1801 atgaacagtt tgccagtgtg gccatctttc gcccactcaa tatccatcgg gcgaccgtag
     1861 tgtttctcaa tttgtacggc ctgttttgcc agttcctgca cttcttcgtt ggtcagcgag
     1921 aagatgtcac gctgttcctg cggtacgtct tcgattttaa cctgcttgcc gtgctcctgg
     1981 gtcggcgcgt aaaccatgcg gatttttttc gaccccatgg tgcgacgcac gatagccggg
     2041 cgattcgccg ccagtgtcgg tttatggacg taaaactcat ccgggttaac cgcaccctgc
     2101 acgaccattt ccccaaggcc ccatgcggaa gtgataaaca ccacctggtc aaagccggat
     2161 tcggtatcaa tggagaacat cacgccagaa gatgcgaggt cggagcgcac catccgctga
     2221 acaccggcag agagcgccac gccacggtga tcgtaaccct ggtgcacacg ataagagatg
     2281 gcgcgatcgt taaacagaga agcaaataca tgcttcactg ccacgagaac ggcgtcaaaa
     2341 ccctgaacgt tgaggaaggt ttcctgctga ccggcaaaag aagcgtccgg catatcttct
     2401 gcggtggcgg aggagcgcac cgcaaaagag gcgttttcgt catcggcgga aagctgtgca
     2461 taggcttcgc ggatggcgtt ttccagctca ggctggaagg gagtgtcgat aatccactgg
     2521 cggatttgcg cgcccgcttt cgcaagctga gtaacatcgt caatatccgt tttatccagc
     2581 agttcataaa tgcgctggtt tacgccgctt tggtccagaa actggttaaa cgcgtcggcg
     2641 gttgtggcga aaccattcgg aacggaaaca cccattccgg aaaggttagt aatcatttca
     2701 cccagggagg catttttgcc cccaaccctg tctacatcat tcatgccgag ttggttatac
     2761 caaagcacca gcggtgacga gccattgttg gacatcgaac aatccttttg tgataaatga
     2821 acggtttgag aaacacattt ctgcgcattt atctttgcat atttaaccgg atgaaaaaaa
     2881 cggtgaatcg ttcaagcaaa tatatttttt tactttttaa gactgatccc agcgttgcgc
     2941 aaatctgctc tcttcgacaa tttccacata aaccatcggt ataaacgaaa ccataaaaat
     3001 gaaatgctgt tttcataaaa aataaaattg aaggttcatt ttataaacca gacataacgt
     3061 cctacgcttc tgtgcgtttt taatttatgc tttcatagaa ttatgtctgc atcacgggaa
     3121 gtacaaaatg gataatgctg ttgatcgcca cgttttttat atttctgatg gtacggcaat
     3181 aactgcggag gtattaggac acgcagtaat gtcacaattt cccgtcacta tcagcagcat
     3241 tacgctgccg tttgtcgaaa atgagagccg tgcacgggca gtgaaggatc agattgacgc
     3301 aatttatcac cagacaggtg tgcgcccgct ggtcttctac tccatcgtgt tgccggagat
     3361 tcgcgccatc atcttgcaaa gtgaaggctt ttgccaggat atcgttcagg cgctggttgc
     3421 cccgctacaa caagagatga aactggatcc aacgccgatt gctcatcgta cccatggcct
     3481 taaccctaat aatctcaata aatatgatgc gcgcattgcg gcgattgatt acaccctcgc
     3541 ccacgatgac ggcatttcgt tgcgcaatct ggatcaggct caggtgatcc tgctcggtgt
     3601 ttctcgctgt ggtaaaaccc ccaccagtct ttatctggca atgcaatttg gtatccgcgc
     3661 ggcaaactac ccctttattg ccgacgatat ggataatctg gtgctacccg cgtcgctcaa
     3721 accgcttcag cataaattgt tcggcctgac tatcgacccg gaacgtctgg cggcgattcg
     3781 cgaggaacgt cgggagaaca gtcgctatgc ctcgcttcgt cagtgcagga tggaagtcgc
     3841 ggaagtggaa gcgttgtacc gtaaaaatca gatcccgtgg attaacagta ccaattattc
     3901 ggtagaagag attgccacca agatcctcga tatcatgggc cttagtcgcc gaatgtacta
     3961 gagaactagt gcattagctt atttttttgt tatgatgcta accacccggc gaggtgtgac
     4021 acacctcgca cttgaaatca gcagcgattg gtttatcgtg atgcgcatca cttcccggca
     4081 gtcctgccgt agaagcaaca aatttctgag acttgtaatg aacagaactg acgaactccg
     4141 tactgcgcgt attgagagcc tggtaacgcc cgccgaactc gcgctacggt atcccgtaac
     4201 gcctggcgcc gccacccatg tcaccgactc ccgccgcaga attgaaaaaa tactcaatgg
     4261 tgaagataag cgactgttgg tcattattgg cccctgctcg atccacgatc tcaccgctgc
     4321 aatggagtac gccccccgtc tgcagtcgct gcgcaaccag taccagtcac ggctggaaat
     4381 cgtaatgcgc acctattttg aaaaaccacg aactgttgtc ggctggaaag gactaatctc
     4441 cgatccagat ttaaacggca gctatcgggt aaatcacggt ctggagctgg cgcgcaaatt
     4501 acttttacag gtaaatgagc tgggcgtccc aaccgcgact gagttcctcg atatggtgac
     4561 cggtcagttt attgctgatt taatcagttg gggcgcgatt ggcgcacgta ctaccgaaag
     4621 tcagatccac cgtgaaatgg cttcggcact ctcctgtccg gtaggtttta aaaatggtac
     4681 cgatggcaat acgcggattg ctgtggatgc tatccgcgca gcccgcgcca gccatatgtt
     4741 cctctcgcca gacaaaaatg gtcagatgac catctatcag accagcggca acccgtatgg
     4801 ccacattatt atgcgtggcg gcaaaaaacc gaattatcat gccgatgata tcgccgcagc
     4861 ctgcgatacg ctgcacgagt ttgatttacc tgaacatctg gtggtggatt tcagccacgg
     4921 taactgccag aagcagcacc gtcgccagtt agaagtttgt gaggatattt gtcagcaaat
     4981 ccgcaatggc tctacggcga ttgctggaat tatggcggaa agtttcctgc tcgaaggaac
     5041 gcaaaaaatc gtcggcagtc agccgctcac ttacggtcaa tccattaccg acccgtgtct
     5101 gggctgggaa gataccgaac gcctggtcga aaaactcgcc tctgcggtag atacccgctt
     5161 ctgaatgcgt gcccattcct gacggaatgg gcatttctgc gcaacttgtt gtcttctcaa
     5221 caaattactg cttgctctgg cctgccataa tattgataat aagaatcatt gttatatcaa
     5281 ttattattaa tctttatgcg ttatacggat agcagaaaac tcacgcctga aacggacgcc
     5341 aatcacaagg ccgcttcccc gcagcccatc cggcgaatta ccagccagac actgttaggt
     5401 ccggatggca aactgattat cgatcatgac gggcaagaat atctgctccg taaaactcag
     5461 gctggcaagc tgctgttaac taagtagcct ttaactcgag cagctgactt ccagccgttt
     5521 accccagtca ggtggacgac tgacatagtc atcatctcta tcgctgaaag gatttcgcaa
     5581 cgccccatgc aggcggtgca attccgtcat atcacccttt tctgccgctt caatcgcccg
     5641 ttgcgccaac cagttgcgca ataccagagc ggggttaacg ctttgcatca actgctgacg
     5701 ctcactatcg ctaacctcgt cttgttgcaa acgcccccga taacgggcaa accagccatc
     5761 aaatgccgca cgatcaataa actcatcacg tagcggtgac gccgtgctgt gctgctcggt
     5821 cagactcagc atgcggaatg tgcgggtata atcgctgcgc tctcgcgcca tcagactgaa
     5881 taattcattc agtagcgcgt tatcctcttt ttgctccgtg atgaagccca gtttctgccg
     5941 catccgttgt ccataatgcg tcaacaaaac ctgctgatag ctgtccaggg cctcattcag
     6001 ggcatctacg gcaacaaatg gtgacaatgt ctgcgccaga cgctgtaaat tccacaacgc
     6061 gacagcaggt tgattatcaa aactataacg cccttgatga tccgagtgat tacaaataaa
     6121 accgggttcg taatcatcaa gaaaaccaaa cggcccgtaa tcaagcgtca gtcccagcag
     6181 cgacatgttg tcggtattca tcaccccatg tgcaaagccg accgtctgcc attgggcaat
     6241 taacgacgcg gtacgtgcga caacatcgct aaaccagaga cggtatttgt cctcatcatc
     6301 ttcaagatgt gaccagtaat gacgaatagc aaagtcagct aactgacgaa ccttttccga
     6361 ctcgcggcgg tagtaaaaat gttcgaaatg accaaagcgc agatgacttg gtgccacacg
     6421 catcagcatc gcgcctggct ccgccgtttc ccgatacact ggcgaatcgc tggtgacgat
     6481 acttaacgcg cgggtcgtcg gaatgcccag ataatgcatc gcctcactgg cgagactttc
     6541 tcgtatcgtc gaacgtaaaa ccgcccgtcc atcacccatt cgcgaataag gcgtcaggcc
     6601 agcacctttc agatgccagt ccattgtagt gccatcagca agcagttgtt cgccgagtaa
     6661 aatgccgcgc ccatcaccca gttggcccgc ccagacgccg aactgatgac cactgtaaac
     6721 ctgcgccagt ggtgacatac caggcagtag ggcttcgccg ccccagacac ctgcgccatt
     6781 tttaaacagc gacgatggaa tactcagcgt gttagccagt tcggtattat gccaaatcag
     6841 ccgggcatta tttaaaggcg taggggaaag tgctgtatag gtttctggca attcatcgcg
     6901 ccagcgggta acaaaagaca gggtcataga tcctcctgtt tgatagtgta gacggttact
     6961 ctcgtcttaa acaccagcaa acagaagggt tatcgctgaa ccaacgtcgt tatctgattt
     7021 atgggaacgg caggccacag gcatccctgg agagcatgga aatcgaaggg ggtaatctgt
     7081 gccaatattt ctgccgtatc aatgcctccc gcaatgatgc aattgcaaca aggggaaatt
     7141 tgcgcctgga tcgcacgaat aaacggttcg aaggagcggt gcgtgatttg ttgctgaata
     7201 aaacttttat ccagcatgac gcgggtgaaa agaccatcaa aaaccgcttt cattgtgcta
     7261 ttgcctgctc ccaaattacc taacacaagg ggatacactt gcgataagga taaaagaccg
     7321 cgattatctt ttccttcatt gagatgcggg taattctcat taatcaacag ttcaataaac
     7381 ggatatttta atagttcgcc tgcgtaatta tccctctcta ataataatgt agccacttgt
     7441 ggcgttaaat taagccaggc aaagagttta tgttgaataa aaaaatgttg gcatgatttt
     7501 agtaattcca actgctctga aaataattgc caatgttgct cctcggtaag ttgcgctata
     7561 acccgactcg tcgggatacg caccgtgcca tcttcactgg agaaatgggt aattaactca
     7621 acgccgacaa ggacttgctg gttatctctt atcggcagga aatagcaatc agaatgataa
     7681 agattctcca aaaaaatctt catggtaacc gtccctctcg aaggatgttt ttcagtatcc
     7741 ggctaaaagg gatgaggcca taagacataa caattacaga aggagtaact ttcatttgtt
     7801 ccatgttaac cactttttca ggggtccttt ttttagatta tcctgattat aaacgaataa
     7861 tccatttacg gaatttttgt ctgcaaaata ctactgtatt caggagtaaa tggacggcga
     7921 tgtatcatcg ccgtccattt ttcgaca
//
LOCUS       DAJVRF010000161         7944 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJVRF010000161 DAJVRF010000000
VERSION     DAJVRF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.161"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            111..443
                     /locus_tag="OQN13_003464"
     CDS             111..443
                     /locus_tag="OQN13_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCS1616734.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            590..1951
                     /gene="yegQ"
                     /locus_tag="OQN13_003465"
     CDS             590..1951
                     /gene="yegQ"
                     /locus_tag="OQN13_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCS1616735.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2281..2598)
                     /locus_tag="OQN13_003466"
     CDS             complement(2281..2598)
                     /locus_tag="OQN13_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616736.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            3004..3903
                     /gene="yegS"
                     /locus_tag="OQN13_003467"
     CDS             3004..3903
                     /gene="yegS"
                     /locus_tag="OQN13_003467"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCS1616737.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            complement(3985..4758)
                     /locus_tag="OQN13_003468"
     CDS             complement(3985..4758)
                     /locus_tag="OQN13_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1616738.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(4864..5904)
                     /gene="gatD"
                     /locus_tag="OQN13_003469"
     CDS             complement(4864..5904)
                     /gene="gatD"
                     /locus_tag="OQN13_003469"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS1616739.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(5952..7307)
                     /locus_tag="OQN13_003470"
     CDS             complement(5952..7307)
                     /locus_tag="OQN13_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCS1616740.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            complement(7311..7595)
                     /gene="gatB"
                     /locus_tag="OQN13_003471"
     CDS             complement(7311..7595)
                     /gene="gatB"
                     /locus_tag="OQN13_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCS1616741.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(7626..>7944)
                     /gene="gatA"
                     /locus_tag="OQN13_003472"
     CDS             complement(7626..>7944)
                     /gene="gatA"
                     /locus_tag="OQN13_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCS1616742.1"
                     /translation="EFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADD
                     DNDVEVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLD
                     SDE"
BASE COUNT         2148 a         2036 c         1783 g         1977 t
ORIGIN      
        1 tgacattatc tctccgccct ggcaggcatc agaaaatcgc tcatacttta atcggtaaac
       61 agcaccttta gatgctgttt tcgatacgca atttcaatca aggagtcatt atggctggtt
      121 ggtttgaact cagtaagagc agtgataatc agttccggtt tgtgctaaaa gcgggcaatg
      181 gtgagactat cctcaccagc gagctgtata catcaaaagc ctctgcggaa aagggcatcg
      241 cgtcggtgcg tagcaacagc ccgcaagaag aacgctatga gaaaaaaacg gcaagtaacg
      301 gcaaattcta tttcaatctg aaagccgcca atcatcaaat tatcggctcc agccagatgt
      361 acgccaccgc gcaatctcgt gaaaccggaa ttgcctccgt taaagccaat ggcacaagcc
      421 agacggtgaa agacaatacg taattacgat gccgggcgtg gggtgtcggc gtccggcgct
      481 ttaccagatc cccccacagc gctacaatgc ccgcccttaa agtgggggca ctcccctaac
      541 cgcttcatca ggtgaagcgg agctgacctg tcatcagaac gagagaatta tgtttaaacc
      601 ggaactcctt tccccggcgg gaacgctgaa aaatatgcgt tacgctttcg cttatggcgc
      661 agatgctgtt tatgcgggcc agccgcgtta ctccctgcgt gtgcgcaaca acgaattcaa
      721 ccacgaaaat cttcagctcg gcatcaatga agcccacgcg ctggggaaaa agttttatgt
      781 cgtggtcaac attgcaccgc acaacgccaa gctgaaaacc tttatccgtg acctgaaacc
      841 ggtggtggaa atggggccgg atgcgctgat tatgtccgat ccagggctga ttatgctggt
      901 gcgtgagcac ttccctgaaa tgccgatcca cctctcggtg caggctaacg ccgtaaactg
      961 ggcgacggtg aaattctggc agcaaatggg cctgacccgc gtgatcctct ctcgcgagct
     1021 gtcgctggaa gagattgaag agatccgcaa tcaggtgccg gatatggaga ttgagatctt
     1081 cgttcacggt gcgctgtgca tggcctactc cggtcgctgc ctgctctctg gctatatcaa
     1141 caagcgcgat ccgaaccagg gtacctgcac caacgcctgc cgctgggagt acaacgtcca
     1201 ggaagggaaa gaagatgacg ttggcaacat cgtacacaag tacgagccga ttccggtgca
     1261 aaatgttgag ccgacgctag gtatcggcgc gccaaccgac aaagtgttta tgatcgaaga
     1321 agcccagcgt ccgggcgagt atatgaccgc gtttgaagat gagcacggca cttacatcat
     1381 gaactcgaaa gatctgcgcg ccatcgccca tgtagaacgc ctgaccaaaa tgggcgtgca
     1441 ttcgctgaaa atcgaaggcc gtactaaatc tttctactat tgcgcacgca ccgcacaggt
     1501 ttatcgtaaa gctatcgatg atgccgctgc gggcaaaccg ttcgatacca gcctgctgga
     1561 aactctggaa ggtctggcgc atcgtggcta taccgaaggt ttcctgcgtc gtcatactca
     1621 cgacgattat cagaactacg aatacggtta ttcagtttct gaccgccagc agtttgttgg
     1681 tgagtttacc ggtgagcgca agggggacct cgcagcggta gcggtgaaaa ataaattctc
     1741 cgttggcgac agccttgagc tgatgacgcc gcaaggcaac attaacttta cccttgagca
     1801 catggaaaac gccaaaggtg aagcgatgcc ggtcgcacca ggcgatggtt atactgtgtg
     1861 gctcccggtc ccgcaggatc ttgagctaaa ttacgcgctg ctgatgcgta atttctccgg
     1921 ggaaaccacg cgtaacccac acggtaagtg attaatttcg attatttttc ccggatggaa
     1981 aattcttaga aaccgatcac atacagctgc atttattaag gttatcatcc gtttcgctga
     2041 aaaacataac ccataaaatg ctagctgtac caggaaccac ctccttagcc tgtgtaatct
     2101 cccttacacg ggcttatttt ttatgcataa gccctatccc tggtcaccgt cttccattga
     2161 ccacatcgat agaatctccc ttcatagcac gatgcctttc acgtaacggc atcgtgctcg
     2221 cacaggttcc ggctaagcac aaccagaacg cgcatgtttg atgcttacca aaaaatattc
     2281 tcactctcca catttgaatg tcagacgagc gacgccatgt aatcctgcac cttctgtctt
     2341 caggtcaact atctgcattt ttttgccctg agtaacacag aaatgggctg catcattttt
     2401 tactatattt tctgcaccag atattcttcc cctagctaaa gaagcttcgg cttcggtgta
     2461 gtattggtta tcgagtttac gctgaatatt acttttatat gcaagaccaa atttaccgat
     2521 acttgtttca tcattatgca cagcacaacc agatataaga aaaatactaa ttaatgatat
     2581 agcagctatc tttttcatct caccttcccc cattaaatac caacgacact ctctagtgtt
     2641 taaatataat aatggcatga ttattataat tgaataggat tataataaat gttctgtaca
     2701 acatttccta cataagtagg aattacggac attgaggccc ttcagggtaa ctccatgggg
     2761 gctttaatat attatattga agatgccact gtttagttga atattaggta tatgctcttt
     2821 tttgaaattt atcggtggca gccgttagta ttcgctgtcc ccattgcaag ctcctggtgg
     2881 taaccactga atcctccata cttgaactga ctttttatcc tccgactttc atcctgttct
     2941 gactccacct tttgttttct gctctacact atctacagac caatcataaa ggcacatacg
     3001 atcatggcag aatttcccgc cagcttactg attcttaatg gcaaaagtac tgacaatcta
     3061 cccttgcgcg aagcaattat gctgttgcgt gaggaaggaa tgacgatcca tgtgcgggtc
     3121 acctgggaga aaggcgatgc cgcacgatat gtagaggagg cccggaagtt gggcgtcgca
     3181 acggtgattg ccggtggtgg cgatggcacc attaatgaag tttctacggc gttgattcag
     3241 tgtgaggggg atggcatacc cgcgctggga attttgccat taggaaccgc caatgatttt
     3301 gccaccagtg tagggattcc tgaggcactg gataaggcgc tgaaactggc aattgccggt
     3361 aacgccattg cgatagatat ggcgcaggtc aacaaacaaa cctgttttat taatatggcg
     3421 acaggcggat ttgggacgcg tattaccaca gaaacgccgg aaaaattaaa agccgcgctg
     3481 ggtggcgtct cttacatcat tcatggctta atgcgcatgg atactctgca accggaccgt
     3541 tgtgaaatcc gcggtgaaaa ctttcactgg caaggtgacg ccctggtcat tggtattggt
     3601 aacgggcgtc aggccggtgg cggtcagcaa ttgtgtccga acgcgttaat taacgatggc
     3661 ttgctgcaac tgcgcatttt taccggcgat gaaattcttc cggctctcgt atcaacctta
     3721 aaatctgacg aagataaccc gaatattatc gaaggcgctt cgtcgtggtt tgatatacaa
     3781 gccccacacg aaatcacttt taatcttgat ggcgaaccgt tgagcggaca aaactttcat
     3841 attgaaatac ttccggcggc gttgcgttgt cgattaccac cagattgtct attattgcgt
     3901 taatcaaact attctctctt gaaccaggga gaatccgaaa tgccggatgc ggtgtaaata
     3961 ccttatccgg catacagaac aatactacac cgtaattaat tttacccgtg tcttctgcaa
     4021 tgccagttta tccacctctg atacagcatc atcggtaata ataatgtcaa ttttttcgat
     4081 tggcagtacc tgattaaaac cgcgacggtt aaatttcgat gaatcgagaa cggcaatcac
     4141 tttattagcg gcggtgacca tcgcaccgct aatcgaataa ccttcattaa aggtcgtaat
     4201 accattaacc gcatcaatac catcagcacc gacaaacatt aaatcggcat taatatcctg
     4261 caatgaacgc tcggcaatgg aaccatgcat cgagcgcgtt ttatggcgga ccgtgccacc
     4321 acagacgacc agagtaatgt ctttattttc ggaaagggca aacgctgccg ggagactgtt
     4381 ggtaatcacc gtgatatttt tagcggtcat taatccttcg gcgataagca ttgtcgtact
     4441 tccgctgtcg agaataacgg tcatcccttc atggatcatt ttgactgccg cctgcgctat
     4501 tctgtttttt ggcgcgctgg cgagctgaaa tcgctcttta aacccgactt cctgggtctc
     4561 ggtttcacta ttaccggaca ttattttcgc cgcaccgcca tgaaagcgcg taaccacgcc
     4621 tttttgttcg agaaagcgca aatcggcacg gattgtcgct tccgaggcag caaatactcc
     4681 cgccagatcc tgaacaagca cggttccctg ttcattcact aattggatga tcttattcct
     4741 tcgctcgaat gaattcatcg tcatttaccc atattcaatt gtggctagtg taaacgaagg
     4801 gggaccattg gtgaataatc tgttttgcac aataccgggc cagcatcacg ctggcccgcg
     4861 gtttcatgga atgagcaaca ctttgcccgg catggcatta cgagcgatgt cacgcaccgc
     4921 ctgggtgaag ctttcaaagc ttccacggtg agcgattaat ggctccaggc ttaacttacg
     4981 ttctgtcagc aaccggctcg ccgtttccca ctcctgcccc ggccaagggc tggaatagtt
     5041 catccaactg ccgataaccg tcagctcttt acgcaatatt ttgccaaacg ttgccgatgt
     5101 taaatgcaga tcctgatgca acgtgcccac cagcgccagt tgggcatgag gaccggcaat
     5161 ctctaccgcc agttcgacag tttgcggtac gccagccgtc tcgaggataa gctgattaaa
     5221 gcgcagttcg cgtaaaacgc tctgcatttg cggcgcgctc atttcgctac tgttaaatgt
     5281 ttgcatcgca ccgaaagatt ttgccaatgc cagtttttct gagctaatgt cgatcgccgt
     5341 cacactcttt gctcccagcg cgacagcgca ctgaatagcc agcaggccaa tggttccggc
     5401 accaataata ataacgtttt tattctcaca accttgcgct aaatgaaaag catgcaggcc
     5461 aacggtaatc ggctcaataa aagccccatc ctcaataggc atatccgtgg gtagagcaaa
     5521 gacatttttt cgcttaacga caatatattc agcaaatcca ccatcacgcc gcgagccaat
     5581 aaaatcatat tttgcgcact gggaataaaa ccctttcaaa cactctggat aagtaaaaca
     5641 gggtaataac ggcacacagg caaccgcatc gccagggtgt aaatcatcaa caccggatcc
     5701 caccgcatca atatagccgc taaattcatg gcctaacgtt attggataat aatgtgcacc
     5761 atttttaaat atcctgggta aatcggaacc acataagccc gagctggcaa tttttacccg
     5821 cacctcatcc tgatgtttaa tttcaggaat gacgctttct gcaacgcgca cgataccatc
     5881 agtatcattc accattgatt tcataaaaac tcctgattgt taagggggat aacctccccc
     5941 tcaggtaaaa attattctgc gagaacggtt ttctcttgtt taataaaacc acgcgctcta
     6001 cgccaggtca tgaaaatacc ggtaagataa attgcgccga taataatgaa accgggaata
     6061 ttttgcgtgg agaaaacctg aatcagtaac caggtaatgg gagaaccgcc ctgatccatt
     6121 gaagccacca tacccccggc ttttaacgcc ccagcattag ccgccagttg ggtgtgcaaa
     6181 ccaatagttt gcgtcgcgat ccacagggtg atgctcataa tgatgacacc cgagattaag
     6241 gtgcggaaca gatttccacg atgcaccgcg accgccatcg ccacgaagaa gccgatggtg
     6301 gcaagatcgc caaacggcag tacctgatta cccggaacac aaacagcaat taaaatggtg
     6361 agcgggataa aaatcaggct tgccgatacc accgccgtat gccccagcag taatgcggga
     6421 tccaggccaa tcaggaactc ctgaccgccg aacttcgcct gtaaacgact acgggcctgc
     6481 ttagcgatgg gcgttaaacc atccatgatg ggtttaatca cccgtggcat cagcagcata
     6541 actgccgccg tttttaccgc cagctgcaat acacctttga catcgtaacc ggcgaggatg
     6601 ccgataatca gccccatcac aaaacccacg gtgacaggct cgccaaatgg accaaatttg
     6661 cgctgaatat cgtcggcgct aaatttaatt cggttaacgc ctgggatttt ctcgatgata
     6721 gcatcgacca gcaccgcaat cggccccata tacgccgacg taccgtgcgg aatagcaata
     6781 ccttccagct caaagaaatt tcgggtatcg cgggcaaacc agtcgccgag cttataaaca
     6841 aacgccgcgt gaattaccac acctgccatc cctatcatcc atgaaccggt tgccagatgc
     6901 agcaacgcgc cggtgaaggt catatgccag atattccaga tatcaacatt taccacccgc
     6961 gtcatacggg tcagtaacat cgccacgtta accagaatcg caatcggaat cgccaccagc
     7021 gcaatttgcg acgcccaggt cattggtgaa gagcccggcc agccgacatc gaccacatgc
     7081 agattcaggt cgaaattttc cgccatcgct ttcgccgccg gaccaatgga atccagcatt
     7141 aagccaatca caaggccaat gccaacaaag ccaatcccga tatgcagacc cgctttaaag
     7201 caatcgcctg ccttcatgcc taatatttta gaaaaaataa tgatgacaat cggaagcatc
     7261 accgtagggc cgaggtcgag aatataacgc atgacttctg aaaacatagg tcacccctgt
     7321 aagatagtca gaattttatt ttgtaatgct tcgataccga caccagaaat aaaaggcatg
     7381 ccgtgaacta acggaatatc gccaaaacta cgatccactt tggcagtggt gcatatcaaa
     7441 tgcacaccat ccatataggt ttctatttca ttaacccgac actggattaa ttcaacagga
     7501 atactatgat tctgacacaa ctctttaatt tcttccgccg ccatcgtaga ggtcgcaacc
     7561 gcgcctccgc aagcgacaat aatcttgcgt ttcataaata gtccttttta ttgttacaga
     7621 gggatttatt cgtctgaatc taaaacatac tttgtgaagt attcctttaa ctgggtttca
     7681 ggaagagtga ttagtgtctc gacgatatcg ggctgttgta acttgccaaa taaacagcgt
     7741 aaaagtttca attgctgctg cggattctcc acaatcaacg caataaccaa cgatacctcc
     7801 acgtcgttat catcatccgc ttgctgaaaa tgaactttat ttgttggcct taacagataa
     7861 atggctgacg acttagcatg aatcgcctca caatgcggta ttgcaatggc gtgctgctca
     7921 agcattatcc cggtagggaa ttct
//
LOCUS       DAJVRF010000162         7939 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJVRF010000162 DAJVRF010000000
VERSION     DAJVRF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.162"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..120
                     /locus_tag="OQN13_003473"
     CDS             <1..120
                     /locus_tag="OQN13_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCS1616743.1"
                     /translation="AINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
     gene            complement(162..932)
                     /gene="yafV"
                     /locus_tag="OQN13_003474"
     CDS             complement(162..932)
                     /gene="yafV"
                     /locus_tag="OQN13_003474"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCS1616744.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            1113..1559
                     /gene="ivy"
                     /locus_tag="OQN13_003475"
     CDS             1113..1559
                     /gene="ivy"
                     /locus_tag="OQN13_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCS1616745.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(1602..4046)
                     /gene="fadE"
                     /locus_tag="OQN13_003476"
     CDS             complement(1602..4046)
                     /gene="fadE"
                     /locus_tag="OQN13_003476"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCS1616746.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4286..4864
                     /gene="lpcA"
                     /locus_tag="OQN13_003477"
     CDS             4286..4864
                     /gene="lpcA"
                     /locus_tag="OQN13_003477"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCS1616747.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5070..5837
                     /locus_tag="OQN13_003478"
     CDS             5070..5837
                     /locus_tag="OQN13_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCS1616748.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(5808..6548)
                     /locus_tag="OQN13_003479"
     CDS             complement(5808..6548)
                     /locus_tag="OQN13_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCS1616749.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(6704..6982)
                     /gene="yafQ"
                     /locus_tag="OQN13_003480"
     CDS             complement(6704..6982)
                     /gene="yafQ"
                     /locus_tag="OQN13_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HCS1616750.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            complement(6985..7245)
                     /gene="dinJ"
                     /locus_tag="OQN13_003481"
     CDS             complement(6985..7245)
                     /gene="dinJ"
                     /locus_tag="OQN13_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HCS1616751.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            7431..>7939
                     /locus_tag="OQN13_003482"
     CDS             7431..>7939
                     /locus_tag="OQN13_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCS1616752.1"
                     /translation="MHDQPLLRMSLPSIPSFVLSGLLLLCLPFSSFACATTSHISFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEA"
BASE COUNT         1948 a         2109 c         1988 g         1894 t
ORIGIN      
        1 gctattaata ttttgacgaa tgataaggta ttcaaggcag ggttaagacg taagatgcga
       61 aaagcagcta tggacagaaa ctatctggcg tcagtccttg cggagagcgg gctttcgtag
      121 tcttaccccg actctccccc agccttaaac acaaccccca ctcaccgcaa cctaaactca
      181 tccgcatcct gccatgccgg aaacttttct ctatattccc gcaatgccat catcgacagc
      241 tccgcatcaa tgcgcgttgc ctgatgcgcg tcggcagtag cgataatctc gccttgtgga
      301 ttaatcaccc ggctgtcacc gcgataatgg cagccgttgc catcgctgcc gacgcgattg
      361 catcccgcca catacgcctg attctcaatc gcgcgggccg tcagcaatgc ctgccagtgc
      421 agagagcgcg gagcaggcca gttggcaacg tagatggcga ggtcataatc gttgagattg
      481 cgcgaccaca ccggaaaacg taagtcgtag cacaccaacg gcaaaatacg ccagccgcgc
      541 cattccacaa tcactcgcgc attgcccgct ttataatgta gatgctcatc tgccatgcgg
      601 aacagatgac gcttatcata aaaatgtacc gtgccgcccg gctcaaccag caaaaagcgg
      661 ttaaccgaac cagactccgt ttgtaatgca acactgcctg caatcagcgc attgcactgc
      721 tgcgccttag ctgtcatcca gtccactacg tcatcttgtg ctagcgacga agctgccgct
      781 tccatggcaa agccgctggt aaacatcttc ggtagaacga tcacatcgcg cccggtaata
      841 ccttccagtt gacgatcaaa atgacgcagg ttggcagggc catccatcca caccagtggt
      901 tgctgcaaaa gcgtaatctt caaaccaggc acggtgtaca actcctttat gcgaagggtt
      961 ttataacttt aacaccttat caggcagttg ccttagcgca gaataaattg ataacaaatg
     1021 ctgatattgg aaatatctga tttgcaaatt atcgtgttat cgccaggctt tagaaggtta
     1081 ataacatggg caggataagc tcgggaggaa tgatgtttaa gacaataacg acagtcgccg
     1141 ctctggtcat cgccaccagt gcaatggcgc aggatgattt aaccattagc agccttgcaa
     1201 agggcgaaac caccaaagct gcatttaatc agatggtaca agggcataag ctgcctgcct
     1261 gggtgatgaa aggcggtact tatactcccg cacaaaccgt aacgttggga gatgatacgt
     1321 atcaggtgat gagcgcgtgc aaaccgcatg actgtggctc gcaacgtatc gctgtgatgt
     1381 ggtccgagaa atctaatcag atgacggggc tgttctcgac tattgatgag aaaacgtcgc
     1441 aagagaaact cacctggctg aatgtgaacg atgcgctttc gattgatggt aaaacggtgc
     1501 tgttcgcggc gttgaccggc agcctggaaa accatccgga tggctttaat tttaaataat
     1561 tagcggataa agaaacggag cctttcggct ccgttattca attacgcggc ttcaactttc
     1621 cgcactttct ccggcaactt taccggcttc gtcgccagct cttccggatc aaagtcatca
     1681 acgttaatac tgcgcagacg gctttcttca gctttcacca gaatagcggc ttcatcttta
     1741 tcaatcagcc ccttcgccag cgcgttgtgc gccagttcat ccagacgggt aaacggcagg
     1801 tttttaccca gctctttaca gatccgctga tgaattgggt cggcggcaat cacatccacc
     1861 agcgcctctt ccagcaagcc aaccggatta tgctcgctcg gcgtcaggta ctgaccgcga
     1921 ccaatgcggg aacgggtggc gttcggcact tgtaaaatct tcgccacttt atgatccagc
     1981 ttgtcagaag gtgccagata atgacgtccg gtcgggaaga tcaccacatt cagcagcccg
     2041 gcaaccacgc ggttcgggaa gttttgcagt aaatcatcca tcgcctgttc agcctgatac
     2101 agcgcatctt gtacgcccca gtgcaccagc ggcaggtcgg cttcattacg gccttcgtcg
     2161 tcataacgct tcagcacggc agaggcgagg tagagctggc ttaaaatatc ccccagacgg
     2221 gccgagatgc gctcgcgacg tttcaggctg ccgtccagca ctgccatcga gacatcagaa
     2281 agcagggcga ggttggcgct caggcggttc aggtgctgat agtagcgttt agtggcatcg
     2341 ccggttggcg tgctgctggt taaaccgcgc gtcaggccca gccagaagct gcgaactttg
     2401 ttgctaccga cgtgaccgat atgtttgaac aacagtttat cgaacgcgtt gacgtcattg
     2461 ttcttcgccg cttccatctc ttccagcacg tacggatggc aacgaatcgc tccttgtccg
     2521 aagatcatca tgctgcgggt cagaatgtta gccccttcaa cggtgatggc aatcggtgcg
     2581 ccctggtaag cacgcgccag gaagttgctt tgcccgagca taatgccttt gccaccagtg
     2641 atatccatcg catcaataat cgactgctgc ccgcggtggg tacagtgata cttaacgata
     2701 gccgacagca cggcaggttt ttcgccgagc ataatgccgt aggtaatcag cgatgccgca
     2761 gcatccatca cgtaggcatt accggcaata cgcgccagcg gctcttcaat cccttccatc
     2821 ttaccgatag agattttgaa ctgacggcga atgtgagcat acgcgcccgt tgccagcgct
     2881 accgatttca cgccgccggt tgagttggaa ggcagggtga tgccgcggcc taccgagagg
     2941 cactccacca gcatccgcca gccttgcccg gccattttcg gcccaccgat gatgtaatcg
     3001 atcggtacga agacatcttt accgcgcgtc ggtccgttct ggaacggtac gttcagcggg
     3061 aagtggcgac gaccaatttc cacgcccggc gtggtggtcg ggatcagcgc acaggtaata
     3121 cctaaatctt ctgcgccacc cagcagcttt tctgggtcgg agagtttaaa cgccagccca
     3181 agcacagtcg caatcggtgc cagcgtaata tagcgtttgt tccaggtcag acgcatcccc
     3241 agcacctgct ggccctgcca ttcgcccatg cagacaatcc cggtgtccgg aatcgcgccc
     3301 gcatcggaac ccgcttccgg gcttgtcagt gcaaagcaag ggatctcctg accacgcgcc
     3361 agacgcggca gatagtgatc tttctgctcg tcagtgccgt aatgttgcag cagctcgccc
     3421 gggcctaatg agttcggcac gccgacggta atcgccagga tcccgctcac gccggagagt
     3481 ttttgcagca cgcgagactg ggcataagct gagaactcca gtccgccgta ctcttttttg
     3541 ataatcatcg cgaagaagcg atgctcttta aggtacgccc acaactccgg cggcagatcc
     3601 gccagctcat gagtaatctg gaaatcattc gccatccggc aggcttcttc taccgggccg
     3661 tcgagaaacg cttgctcttc ggcggtcagg cgcggctgcg gatagttatg cagctttttc
     3721 cagtccggct tgccctggaa caagtcgccc tcccaccagg tggtgcccgc atcaatcgct
     3781 tctttctcag tgcgcgacat cggcggcatc accttacgga aaccgcgaaa taccggcgcg
     3841 gaaatcatcg acttacgcat aggcgcaaag ttaaatggca cgaggataat ggccagaggc
     3901 accagtaccc acgctgacca cagaccagca acgccgaggg cggctgtcca ggcgagcaaa
     3961 atcagactgc tgataaataa gctcacgcgg tgatagaaca acgcgccgag caggacaacc
     4021 gtagcgagaa tactcaaaat catcataacg aaaagcccct tacttgtagg aggtctgacc
     4081 acttgtgatg atatggttgt agtggatgta aaaacattta gcaatgtgtt tacaatataa
     4141 ttacaacaaa gctcacattg ttgctgtttt tatccgcact tcaggtcaaa aagtcctggt
     4201 catagcacct gcccgtactt ctcgcttttg gcggtatccg gtacactgca ttttgtctat
     4261 tacatttatg ctgaaggata tcctcatgta ccaggatctt attcgtaacg aactgaacga
     4321 agcggcggaa acgctggcta actttttaaa agatgacgcc aatattcacg ccattcagcg
     4381 cgcggcggtc ctgttagcag acagctttaa agccggtggc aaagtgcttt cctgcggcaa
     4441 cggcggttcc cattgcgacg ctatgcactt tgccgaagag ttgaccggtc gctaccgtga
     4501 aaaccgtccg ggctacccgg cgattgctat ttctgacgtt agtcatattt cctgcgtcgg
     4561 taatgatttc ggtttcaatg atattttctc ccgctacgtt gaagcggtag gtcgcgaagg
     4621 cgatgtactg ctggggatct ccacctccgg taactctgca aacgtgatca aagcgatcgc
     4681 agcggcgcgt gagaagggga tgaaagtgat caccctgacc ggtaaagacg gcggtaaaat
     4741 ggctggcacg gcggatatcg aaattcgcgt tccgcacttt ggttatgccg accgcattca
     4801 ggagattcac attaaagtga tccatatcct gatccagtta attgaaaaag agatggttaa
     4861 gtaagtctgg cgtaggccgg ataaggcgtt tacgccgcat ccggcacttg tgcgctgatg
     4921 cctgatgcga cgctgatgcg tcttatcatg cctacaaatc tgtacgcgaa ccgtaggccg
     4981 aataagacgt ttacgccgca tccggcctga aaattcttaa atcaatcttc gccgggggcc
     5041 atgcgctccc gctgttgtgg aggttaccca tgtgcgaatt gctcgggatg agcgccaacg
     5101 tccctaccga tatctgcttt agtttcaccg gtctggtgca gcgtggtggt ggaaccgggc
     5161 cacataaaga tggctggggc attacctttt acgaaggtaa aggctgtcgc acatttaaag
     5221 atccacaacc cagctttaat tcccccatcg ccaaacttgt ccaggactac ccgataaaat
     5281 cctgttcggt ggtggctcat attcgccagg ccaatcgggg cgaggtggcg ctggaaaata
     5341 ctcacccgtt tacccgtgag ttatgggggc gtaactggac ttatgcccat aacggacaac
     5401 tgacgggcta caaatcactg gaaaccggca acttccgccc ggtcggtgaa accgatagcg
     5461 aaaaagcctt ctgctggctc ctgcataaat taacgcagcg ttacccgcgc acaccgggca
     5521 acatggcggc agtgtttaaa tatatcgcct cactggcgga tgaactgcgg cagaagggcg
     5581 ttttcaacat gctgctttct gacgggcgct atgtgatggc gtattgctcg actaatttac
     5641 actggatcac ccgccgcgcg ccgtttggcg tggcaacgct gctggatcag gatgtggaaa
     5701 tcgacttcag ctcgcagacc acaccgaatg atgtggtcac ggtgattgca acacagccgc
     5761 tgacgggcaa tgaaacctgg caaaagatta tgccaggcga atggcgctta ttttgcctcg
     5821 gggagcgtgt agtttgatgc cagttgtggc tgcacaactt cgtggcttaa cggcttgctg
     5881 accacgtaac gaccattgac cacagaaacg gtcggtggct tacgggtttg ctcaaagtag
     5941 tcgtaacccg gcttcagttg ctcccagaag tccttaaagt tggaatattt atggcgcttc
     6001 atattggcgt cggtcatgcg gaacgggtaa atactcactt gcacgctcgg ctgaccaaac
     6061 accagcgcac cagtaacgaa ctggaagatc tcatcaatac cctgattggt cattgcgtag
     6121 cagccgatgg aaacacaatc gccgtggatc atcaggtatt tcccttcata accatgcgca
     6181 cggtcatagg cattggggaa accaatatta atcgctttgt agtaacggct gtctggtttt
     6241 aactgattac gctggacgct ataaaaccct tccgggcttt tgaaatcgcc ctgacgctgt
     6301 tttggcccta agccgccgga atatttacag attttataac tgtcgagcag ctgatattgc
     6361 tcgcccattt tgacgtagag atcgagcgta cgttcttcct tgaagatctg gatgtagaca
     6421 ggggatccca tcaactgctg cttatactct ttgctcactg gcgtggtcga gctactgctg
     6481 cccagcaaac cggcaaacga aacgcacggg atcaacaaca tcgcaagaat taatgcgatt
     6541 ttacgcatac tgcttattcc ttgataaaac ggttacacac gccaggacgg caaaatagat
     6601 cccaaatcgg aatagtctgg atttggaagg ctcacattat caccaaaaga gttttacgca
     6661 agcctgtcgg cgcagggttt acaaatttca tcggcagcgt gctttaccca aagagcgccg
     6721 cgtgagttcc agttctctca aatcgtaaaa gtttatcggt aagtttgtaa atcaggatcc
     6781 agtccggttc gacatgagca tcgcgataac ctttccatga accttgcagc gggtggtctt
     6841 tataaacagc tggaagcggt aaagcattat tgataagaag cgtcataaga tatttcaatt
     6901 tattcatatc cttatgacgc ttttgtgcaa gttttacatc ctttgaaaat tgtcccgagt
     6961 attcaatatc cctttgaatc atatttaaat tcctaattta tcaaataaat catcagcgtc
     7021 tttggcctta tgaacatcaa cgccagcttc gctgtttttg attgattgaa tggttaattg
     7081 attaggctcg cgtaaatcaa acggcaatgc cttttcacgc gcgacctttg tgagggttat
     7141 gcgaaccagg tcagagatgg tcagccccat cccggccagt acgtccgccg cctgattctt
     7201 cagatcttca tcgattcggg cgcgaacaaa cgcgttagca gccattgggg gatctcctgt
     7261 gctgtatatt tattcagctt gaattgtagc tcaaatgagc aacagcgaca atccatgagt
     7321 aatttaataa acggatgttt ttacataata aactgaaata tatttattaa ttttataata
     7381 atggtttatt aagtttgttc aggtacgggc caggatacac tgcaacatca atgcatgacc
     7441 agccattatt gagaatgtct ttgccgtcga ttccctcttt tgtgttgtcg ggattactgt
     7501 tgctttgctt gccgttttct tcatttgcct gcgccaccac atcacatatc tctttcagct
     7561 acgccgcccg ccagcggatg caaaaccgtg cgcgtttatt aaaacagtac caaacccatc
     7621 tgaaaaaaca ggccagctac attgtggaag gcaatgccca aagcagaagg gcgctgcgcc
     7681 agcacaaccg ggagcagata aaacagcatc cagaatggtt tcctgcgccg ctcaaggcga
     7741 gtgacagacg ctggcaggcg ctggcggaaa acaaccactt tttaagcagc gatcacctgc
     7801 ataacatcac cgaagtggcg attcaccgtc tggagcagca gcttggcaag ccttacgtct
     7861 ggggcggcac gcggcccgat caaggcttcg attgtagtgg gttagttttt tatgcctaca
     7921 acaagatcct cgaggcgaa
//
LOCUS       DAJVRF010000163         7936 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJVRF010000163 DAJVRF010000000
VERSION     DAJVRF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.163"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(265..1296)
                     /gene="rsmC"
                     /locus_tag="OQN13_003483"
     CDS             complement(265..1296)
                     /gene="rsmC"
                     /locus_tag="OQN13_003483"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCS1616753.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDMLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1399..1812
                     /gene="holD"
                     /locus_tag="OQN13_003484"
     CDS             1399..1812
                     /gene="holD"
                     /locus_tag="OQN13_003484"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCS1616754.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1781..2227
                     /gene="rimI"
                     /locus_tag="OQN13_003485"
     CDS             1781..2227
                     /gene="rimI"
                     /locus_tag="OQN13_003485"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCS1616755.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2242..2919
                     /gene="yjjG"
                     /locus_tag="OQN13_003486"
     CDS             2242..2919
                     /gene="yjjG"
                     /locus_tag="OQN13_003486"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCS1616756.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3010..4599
                     /gene="prfC"
                     /locus_tag="OQN13_003487"
     CDS             3010..4599
                     /gene="prfC"
                     /locus_tag="OQN13_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCS1616757.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            4992..5597
                     /gene="osmY"
                     /locus_tag="OQN13_003488"
     CDS             4992..5597
                     /gene="osmY"
                     /locus_tag="OQN13_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCS1616758.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5724..5885
                     /locus_tag="OQN13_003489"
     CDS             5724..5885
                     /locus_tag="OQN13_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCS1616759.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6007..7080
                     /locus_tag="OQN13_003490"
     CDS             6007..7080
                     /locus_tag="OQN13_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCS1616760.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7077..7859
                     /locus_tag="OQN13_003491"
     CDS             7077..7859
                     /locus_tag="OQN13_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1616761.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         1895 a         2047 c         2152 g         1842 t
ORIGIN      
        1 ccatcaattc ttaaaaagaa ttgctaccac ggaggcgcat tctagtggtt ttcagctttt
       61 cgtcaatagt taattatcgc cggaggtgta attgctggaa aaatgtccat caggaaacta
      121 gcgtgcaggt ttggtaggca tgcgggggca gatgccagat gcgacgctgg cgcgtcttat
      181 ctggcctacg aagggctaac gtgcaggttt tgtaggtcgg ataaggcgtt cacgccgcat
      241 ccgacacggt attcggcgag ataattaacc tttcttcgcc tggcgggtca taatggcgcg
      301 atacaccttg aaacgcccgg tttgcgcaat cacttcatgg aagccaaatg tctcatccag
      361 cacgtccggg taaggcagga aggcgttcgc tacaattcgc agctcgccgc cgctattaag
      421 atgacgcacc gcgccgcgaa tcagcgtttg cgccgcatcc aggctggttt gcatcccatc
      481 gtggaacggc gggttggaga tgatcatatc aaaacgacct ttcacctcgg aaaagacgtt
      541 gctggcaaag acttcacctt caacacagtt ggccgcaagt gttgcgcggc tggcttctac
      601 cgctggcgca gagacatcgc acaaggtgag acgaattttc ggcgaatggc gcgcaaaggc
      661 aactgaaagc acccccgcgc cacagccgac atccagcact ttacctttcg tgtgcggagt
      721 taacgtcgag agcagcaact ggctaccgac atccagaccg tcgcggctaa acacgccagg
      781 cagcgttttg accgtcaggc catcgacgct gtattcgccc cagaatttat cggcatcaaa
      841 taccggctgt ttttccagac gaccaaaata gaggccacag cgacgagcgc tgtcgacttt
      901 attcaacggc gcataatctg ccagcatctg ctcggcgctg cgcacgccgc tgcggttctc
      961 gccaacgaca aaaatatctg tccccactgg cagcagagaa agtaaattca tcaactggaa
     1021 ctgggcttcc ggtttgttct tcggccagta gtaaatcagc atatcgcaat ctgcgacgtc
     1081 atccaccgtg gcgaccagac tgaaacgggc gttatccccc atctggcggc ttaatacctg
     1141 ccagtggtgg aattgctggg tatgagcacg gctggccgcg gtatctaaac gcgcgggcag
     1201 gtcatcctgt aagtctccgg caaacagaat acggctttgt tcgaaatcat cactgtgacg
     1261 cagcaagact tcacttgccg gggtaaatgc agacatggaa tgctcctcaa ttgatactgg
     1321 cggcgattat agccatatgt tggcgcggta tcgacgaatt tgctatattt gcgcccctga
     1381 caacaggagc gattcgctat gacatcccga cgagactggc agttacagca actgggcatt
     1441 acccagtggt cgctgcgtcg ccctggcgcg ttgcaggggg agattgccat tgcgatcccg
     1501 gcacacgtcc gtctggtgat ggtggcaaac gatcttcccg ccctgaccga tcctttagtg
     1561 agcgatgttc tgcgcgcatt aaccgtcagc cccgatcaag tgctgcaact gacgccagaa
     1621 aaaatcgcga tgctgccgca aggcagtcgc tgcaacagct ggcggttggg tactgacgaa
     1681 ccgctatcac tggaaggcgc tcaggtggca tcaccggcgc tcaccgaatt acgggcaaac
     1741 ccaacggcac gcgccgcgtt atggcaacag atttgcacat atgaacacga tttcttccct
     1801 cgaaacgact gatttaccag cggcttacca cattgaacaa cgcgcccacg cctttccgtg
     1861 gagtgaaaaa acctttgcca gcaaccaggg cgagcgttat ctcaactttc agttaacgca
     1921 aaacggcaaa atggcggcgt ttgcgattac gcaagtggtg ctggatgaag ctacattgtt
     1981 caatattgcg gtcgatcctg actatcagcg tcagggattg ggaagggtgc tgctggaaca
     2041 tctgatcgac gaactggaaa aacgcggcgt ggcgacacta tggctggaag tccgtgcctc
     2101 aaatgctgcc gccattgccc tgtacgaaag tttaggcttt aacgaggcga cgattcgccg
     2161 caattactac cccaccacgg acggtcgcga agacgccatc atcatggcgt tgccaatcag
     2221 tatgtaagac aaggtggaat aatgaagtgg gactggattt tctttgatgc cgatgaaacg
     2281 ctgtttacct ttgactcatt caccggcctg cagcggatgt ttcttgatta cagcgtcacc
     2341 tttaccgctg aagattttca ggactatcag gccgttaaca agccactgtg ggtggattat
     2401 caaaacggcg cgatcacttc attacagctt cagcacgggc gttttgagag ctgggccgaa
     2461 cggctgaaag ttgaagcagg cttgcttaac gatgccttta ttaatgcgat ggcggaaatc
     2521 tgcacgccgc tgccgggcgc ggtttctctg cttaacgcca ttcgtggcaa cgccaaaatc
     2581 ggcatcatca ccaacggctt tagcgccttg cagcaagtgc gtctggaacg cacgggcctg
     2641 cgtgattact tcgatttgct ggtgatttcc gaagaagttg gcgttgccaa accgaataag
     2701 aaaattttcg attatgcgct ggaacaggcg ggcaatcctg accgttcacg cgtgctgatg
     2761 gttggcgaca ctgccgagtc cgatattctc ggtggcatca acgccgggct tgcgacctgc
     2821 tggctgaatg cgcacaatcg cgagcaacca gaaggcatcg cgcccacctg gaccgtttct
     2881 tcgttgcacg aactggagca gctcctgtgt aaacactgat tgcctccccc ccgttgatgg
     2941 gtaaaatagc cgcaattttt cgttttcaac aagcgcggcg cgatgccgct tactcaagaa
     3001 gaaagaatta tgacgttgtc tccttatttg caagaggtgg cgaagcgccg cacttttgcc
     3061 attatttctc acccggatgc cggtaagact accatcaccg agaaggtgct gctgttcgga
     3121 caggccattc agaccgccgg tacagtaaaa ggccgtggtt ccaaccagca cgctaagtcg
     3181 gactggatgg agatggaaaa gcagcgtggg atctccatta ctacgtctgt gatgcagttt
     3241 ccgtatcacg attgcctggt taacctgctc gacaccccgg ggcacgaaga cttctcggaa
     3301 gatacctatc gtaccctgac ggcggtggac tgctgcctga tggttatcga cgctgcaaaa
     3361 ggtgttgaag atcgtacccg taagctgatg gaagttaccc gtctgcgcga cacgccgatc
     3421 ctcaccttta tgaacaaact tgaccgtgat atccgcgacc cgatggaact gctcgatgaa
     3481 gttgagaacg agctgaaaat cggctgcgca ccgatcacct ggccgattgg ctgcggcaag
     3541 ctgtttaaag gcgtttacca cctttataaa gacgaaacct atctctatca gagcggtaaa
     3601 ggccacacga ttcaggaagt ccgtattgtt aaagggctga ataacccgga tctcgacgct
     3661 gcggttggtg aagatctggc acagcagcta cgtgacgaac tggaactggt gaaaggcgcg
     3721 tctaacgagt tcgacaaaga attgttcctt gcgggcgaaa tcactccggt attcttcggt
     3781 actgcgctgg gtaacttcgg cgttgatcat atgctggatg gcctggtgga gtgggctcct
     3841 gcgccgatgc cgcgtcagac tgatacccgt accgtagagg cgagcgaaga caaatttacc
     3901 ggcttcgtat ttaaaattca ggccaacatg gacccgaaac accgcgaccg cgtggcgttt
     3961 atgcgcgtag tatccggtaa atatgaaaaa ggcatgaagc tgcgccaggt gcgtactgcg
     4021 aaagatgtgg ttatctccga cgcgctgacc tttatggcgg gcgaccgttc gcacgttgaa
     4081 gaagcgtatc cgggcgatat cctcggcctg cacaaccacg gcactattca gatcggcgac
     4141 acctttaccc agggtgagat gatgaagttc accggtattc cgaacttcgc accagaactg
     4201 ttccgtcgta tccgcctgaa agatccgctg aagcaaaaac agctgctcaa agggctggta
     4261 cagctttccg aagagggcgc ggtgcaggtg ttccgtccga tctccaacaa cgatttgatc
     4321 gttggtgctg ttggtgtgct gcagtttgat gtggtggtat cgcgcctgaa gagcgaatac
     4381 agcgttgaag cagtatatga atcagtcaac gttgccactg cccgctgggt agaatgtgcg
     4441 gacgcgaaga aattcgaaga gttcaagcgt aagaacgaaa gccaactggc gcttgatggc
     4501 ggcgataacc tcgcttacat cgctaccagc atggtcaacc tgcgcctggc gcaggaacgt
     4561 tatcccgacg ttcagttcca ccagacccgc gagcattaat tcttgcctcc agggcgcggt
     4621 agccgctgcg ccctgtcaat ttcccttcct tattagccgc ttacggaatg ttcttaaaac
     4681 attcactttt gcttatgttt tcgctgatat cccgagcggt ttcaaaattg tgatctatat
     4741 ttaacaaagt aatgacattt ctgacggcgt taaataccgt tcaatgcgta gatatcagta
     4801 tctaaagccg tcgattgtca ttctaccgat attaataact gattcagagg ctgtaatggt
     4861 cgttattcat cacccatcgc ttttgtgatg gcgaccattg acttctgtag agggtgaagt
     4921 ctctccctat tcagcaatgc aacctcgtgt tgccaggctc aaattacgag caaacataca
     4981 ggaataaatc gatgactatg acaagactga agatttcgaa aactctgctg gctgtaatgt
     5041 tgacctctgc cgtcgcgacc ggctctgcct acgcggaaaa caacgcacag actaccaatg
     5101 aaagcgcagg gcaaaaagtc gatagctcta tgaataaagt cggtaatttc atggatgaca
     5161 gcgccatcac cgcgaaagtg aaggcggcgc tggtggatca tgacaacatc aagagcaccg
     5221 atatctctgt caaaaccgat caaaaagtcg tgaccctgag cggtttcgtt gaaagccagg
     5281 ctcaggccga agaggcagtg aaagtggcga aaggcgttga aggggtgacc tctgtcagcg
     5341 acaaactgca cgttcgcgac gctaaagaag gctctgtgaa gggctacgcg ggtgacaccg
     5401 ccaccaccag tgaaatcaaa gccaaactgc tggcggacga tatcgtccct tcccgtcatg
     5461 tgaaagttga aaccaccgac ggcgtggttc agctctccgg taccgtcgat tcccaggcac
     5521 agagcgaccg tgctgaaagt atcgccaaag cggtagatgg tgtgaaaagc gttaaaaatg
     5581 atctgaaaac taagtaattc gtcgtaattc gtcctcccga actttgtcgg gaggcgcgat
     5641 gtgcaccaca ctaaaaatat cgcattgagt gattttcacg ctcatatcaa gcggtgacat
     5701 taactatggt aaaggagacg cttatgtttc gttggggcat catatttctg gttatcgcgt
     5761 taatcgccgc cgcacttggg tttggtggtc tggccggtac cgctgcaggc gcagctaaaa
     5821 ttgtctttgt cgtcgggatt attctgttcc tggtgagttt gttcatgggc cgaaaacgac
     5881 cctagatttc agaccattaa gaaaatttta tccaaagcca gtccagcgga ctggcttttg
     5941 cggttttagc gaataataaa ttgcgttact ttgtcattct ttcacaacaa aaacaggaag
     6001 gcagaggtgg ggcagcgaat acctgtaacg cttggtaata ttgcgccgtt gtcgctaagg
     6061 ccgttccagc ctggacgaat agctctggtg tgcgaaggcg gcggacagcg tggaattttc
     6121 acggctggcg tgctggatga gtttatgcgc gcgcagttta atcctttcga tctttatctc
     6181 ggcacatctg ccggggcgca gaacctctcg gcgtttatct gcaatcagcc cggttacgcg
     6241 cgcaaagtca tcatgcgcta taccacaaaa cgcgaatttt tcgatccatt gcgctttgtc
     6301 cgtggaggaa atcttatcga tctcgactgg ctggtggagg ccaccgcaag ccagatgccg
     6361 ttgcaaatgg ataccgccgc gcggttgttt gacagcggca aatcgtttta tatgtgcgcc
     6421 tgtcgtcagg atgactacgc gcctaattac tttttaccaa ccaaacaaaa ctggctggat
     6481 gtgattcgcg cctccagtgc gatacctggc ttttatcgta gcggagtgtc gctggaaggc
     6541 attaactacc tggatggcgg gatcagtgat gcgattccgg ttaaagaggc ggcaaggcag
     6601 ggcgctaaaa cgttggtcgt cattcgcact gtgccgtcac aaatgtacta cacgccgcag
     6661 tggttcaaac gcatggaacg ctggctgggt gacagtagcc tgcagccgct ggtcaatctg
     6721 gtgcagcatc atgaaaccag ctatcgtgac attcagcaat ttattgagaa accaccgggc
     6781 aagctgcgga tattcgaaat ttatccgccg aagccattac atagtatcgc gcttggcagt
     6841 cggattccgg cgctgcgtga agactataaa cttgggcgtt tatgcggtcg ttatttcctc
     6901 gccacggttg gcaagctatt aactgaaaaa gcgccgctta cccgccatct ggtgccagtg
     6961 gtgacgccgg aatcgatcgt tattccgcct gcgccagtcg ccaacgatac gctggttgcc
     7021 gaagtgagcg atgctccgca ggcgaacgac ccgacattta acaatgagga tctggcttga
     7081 tttgccgttt tatcgatacc cactgccatt ttgatttccc gccgtttagt ggcgatgaag
     7141 aggccagcct gcaacgcgcg gcacaagcgg gcgtaggcaa gatcattgtt ccggcgacag
     7201 aggcggaaaa ttttgcccgc gtgcaggcgt tagcggaaaa atatcaaccg ctgtatgccg
     7261 cattggggtt gcatccgggg atgttggaaa aacatagcga tgtgtctctt gatcagctac
     7321 agcaggcgct ggaaaggcgt ccggcgaagg tggtggcggt gggggagatc ggtctggatc
     7381 tcttcggcga cgatccgcaa tttgagaggc agcagtggtt actcgacgaa cagctgaaac
     7441 tggcgaaacg ctacgatctg ccggtgatcc ttcattcccg gcgtatacac gacaaactgg
     7501 cgatgcatct taaacgtcac gatttaccgt gcactggcgt ggttcacggt ttttccggca
     7561 gcctgcaaca ggccgagcgg tttgtacagc tgggctacaa aatcggcgta ggcggtacta
     7621 tcacctatcc acgcgccagt aaaacccgcg atgtcatcgc aaaattaccg ctggcatcgt
     7681 tgttgctgga aaccgacgcg ccggatatgc cgctcaacgg ctttcagggg cagcctaacc
     7741 gaccggaaca ggctgtgcgg gtgtttgacg tgctttgcga gttgcgcccg gaaccagaag
     7801 atgagattgc cgaagtgttg cttaataata cgtatgcgtt gtttagcgtt agtgggtagc
     7861 ctacccttcg taggcagcat ccggcagtcg gcgcataatg cctgatgcga cgcttaacgc
     7921 gtcttatcat gcctac
//
LOCUS       DAJVRF010000164         7895 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJVRF010000164 DAJVRF010000000
VERSION     DAJVRF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7895
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.164"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..332)
                     /locus_tag="OQN13_003492"
     CDS             complement(<1..332)
                     /locus_tag="OQN13_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCS1616762.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVR"
     gene            complement(419..925)
                     /gene="mug"
                     /locus_tag="OQN13_003493"
     CDS             complement(419..925)
                     /gene="mug"
                     /locus_tag="OQN13_003493"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCS1616763.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVTYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            1050..1125
                     /locus_tag="OQN13_003494"
     tRNA            1050..1125
                     /locus_tag="OQN13_003494"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1083..1085,aa:Ile,seq:cat)
     gene            complement(1179..1943)
                     /gene="nfeF"
                     /locus_tag="OQN13_003495"
     CDS             complement(1179..1943)
                     /gene="nfeF"
                     /locus_tag="OQN13_003495"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCS1616764.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            2231..2854
                     /gene="nfeR"
                     /locus_tag="OQN13_003496"
                     /gene_synonym="yqjI"
     CDS             2231..2854
                     /gene="nfeR"
                     /locus_tag="OQN13_003496"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCS1616765.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHSRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(2961..4481)
                     /gene="aer"
                     /locus_tag="OQN13_003497"
     CDS             complement(2961..4481)
                     /gene="aer"
                     /locus_tag="OQN13_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCS1616766.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4899..6278
                     /gene="ygjG"
                     /locus_tag="OQN13_003498"
     CDS             4899..6278
                     /gene="ygjG"
                     /locus_tag="OQN13_003498"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCS1616767.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMQLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(6320..6652)
                     /locus_tag="OQN13_003499"
     CDS             complement(6320..6652)
                     /locus_tag="OQN13_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCS1616768.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6871..7854
                     /gene="ebgR"
                     /locus_tag="OQN13_003500"
     CDS             6871..7854
                     /gene="ebgR"
                     /locus_tag="OQN13_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCS1616769.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1933 a         2132 c         1983 g         1847 t
ORIGIN      
        1 cggacagcct cacttcccag gcgctggcgg gcctgaataa cggcactggg ggcaacgaag
       61 gggcgattgc ccggcagcat gatgtccagg cgattcacaa tctggtgaag aggttcttta
      121 cgctcaagcg ccatgccaac aatacaccag accatcattt cgaggggaag acggcgcttg
      181 cgtagcgtta cagtacctga ttcggcaagg caacgagaga tgagttcggg gtcgaggtaa
      241 tcccccagag aagtcagtgg gttacgcaga gaatcgtaac gggataccag atcaagagcc
      301 tgtccaatgt gcataaaaaa atccggaaac aagtgagcgt ttccggattc ttacacagcc
      361 actggatcgg tcaactgatc cttaactgat cggcattaca ccttttcggt ggcgtttttt
      421 atcgcccacg cactaccagc gcctggtcca gctcgcgata cgcttcaacc agtttctcta
      481 gtgaaacgcg acttaaaccg ctgggatttg gcagcaccca aatctgcgtc gaaccaatgg
      541 tgagcgtttg tttcccccac tgtgcaccgc gctggctgaa tccctgttca tatgcttgtt
      601 tgcccagaat cgccaacgcc tgcggctgat aatcttcaat tttttcaatc agcttacgcc
      661 cacctgcgtg cagctcctgc tttgaaactt cattggcttg caccgttgga cggtctacca
      721 gtttggtgac gccacaacga taatccagca gatgctgtgc ctcctgcggc ttcaactgac
      781 ggtcggtaaa cccggcctga tatgtcacct tccagaagcg atttgctgga tgagcaaagg
      841 gaaaaccagt cccggcggat gaaagcccag ggttgatacc gcaaaatacg acccgtaacc
      901 ctggagccaa aatatcctca accatattta ctcctttcta acatcaccag gaaaagtata
      961 acggattgaa aatacattgt ttataaaaac agcaggcgca cggtaatggc tggattgcga
     1021 cacggagtta ctttataatc cgctaccatg gccccttagc tcagtggtta gagcaggcga
     1081 ctcataatcg cttggtcgct ggttcaagtc cagcaggggc caccagatat agcaaaggct
     1141 gacgagaaat cgtcagcctt tttcttttta tatatcagtt actttgcgtg ccagtaagcc
     1201 gctgcacgta cccgctgtgg gtcatactgt tccgcttcaa agcggcggct taaattctta
     1261 acgactttac cttcgccggt tatccagatg aagtaatcat cggcagggat ttgcatctgc
     1321 gccagacgcg catctaccgc ctgctcatca tgtgccagcc attcgatatt aaaaccatca
     1381 aggtgcgcga gataatcctg acaggcgtta tcccgcacgc taaccagcgc actaacttgc
     1441 ggtttaacgg caagtttgct caacgtttcc aagcggcggc gcagtgcggg cattccggat
     1501 tcatcgcaga catacagctg atacgcgtaa tcttccggca ccaccagcga accgcgcgga
     1561 cctgcaaccg taagtttatc gcccggttgc gcctgcatcg cccagccgct ggcgacccca
     1621 ccgtcgtgaa taaagaaatc gatcgccagt tcatggcgta gttcgtcata cagcggcgta
     1681 tagtcacgcg acggtgggcg tggtccttcc ggccagacga tgccctcttc cgttaccgtt
     1741 ggcggcacaa agtgagcgtc aggttgagga aagaagagtt tgctgtgatc gtcaaagccg
     1801 cgcgatgtaa aaccgtccag cgcctcgccg ccgaggacaa tgcgctgaaa accggcgctg
     1861 atgcgctcaa cgcgtaacac agtcagttca cggaagcgca gatcattgcg aacgcgctgc
     1921 gggtagcggg gggagttatt cattgttatc gccttcgtga tggtaatcag atatatctaa
     1981 ataattttcg taaatgataa tgattgttaa tcatgataaa tgcaagcgat ttgtagaact
     2041 gatatgtcta tagtctgata agacgaaccg cctcttctca ggcatcatta ctcaacgccg
     2101 gatgcggcgt gaacgcctta tccggcctac gtgtgagatg agtccacatt taaaaatcat
     2161 ttttattctt gcaagagtgc tcatatcaga tataaattag atatatctaa tcaagcaaaa
     2221 ggaggctgat atgagccatc atcacgaagg gtgttgtaaa catgaaggcc agccacgcca
     2281 tgagggctgc tgcaaaggtg agaagtcaga acacgagcac tgcggacacg gtcaccagca
     2341 tgaacacggt caatgctgcg gtggtcgcca cagtcgcggc ggcggtcgtc ggcaacgttt
     2401 ctttggtcac ggtgaattgc gtctggtgat tctggatatt ctctcgcgcg atgacagcca
     2461 cggttacgaa ttgattaaag cgattgagaa tctaacccag gggaattaca ccccaagccc
     2521 gggcgtcatc tacccaacgc tggattttct gcaggagcag tcgctgatta ccatccgcga
     2581 agaggaaggt ggtaagaagc agattgcgct gaccgaacaa ggcgcgcagt ggctggaaga
     2641 aaaccgcgaa caggtggaga tgattgaaga acgcatcaaa gcgcgttgcg ttggtgctgc
     2701 gctgcgccag aacccgcaaa tgaagcgggc gctggataat tttaaagcgg tgctggattt
     2761 acgcgtcaac cagagcgata tcactgatgc acaaataaaa aagatcattg cggtgatcga
     2821 ccacgccgct tttgatatta cccaactgga ttaatcaccg attgccggat gcggcgtacc
     2881 ctacacaccc cgcaggcctg ataagatgcg ccagcatcgc atcaggcatt gtgctccaac
     2941 cgccggatcc ggcataccga ttaatgcagc accgtcaccg cgtcttccag tcggctggcg
     3001 cggtgtttca ccatcgccga cacctgcgca ctctcttcca ccagctcagc atttttctgg
     3061 gtgatcaggt taagctcatc cactgcacgg gtcaggctgg aaagcccatc ggcctgttcc
     3121 agcgttgaat ggctaatctg ggcgatcaac tgggtgacgt ttttcacctg tgccacaata
     3181 tcttccatcg tccgtccggc ggcgtgtacc tgctgcgaac cggattgcac cttatcagca
     3241 ctggcatcaa tcagcttgcg aatatcgttg gcagcattag cgctgcggct ggctaaatga
     3301 cgcacttccc ctgccaccac tgcaaaacct ttgccctgtt cgccggcacg cgccgcttcc
     3361 accgccgcat tcagggccag aatattggtc tgaaacgcaa tatcgttaat cagcgaagta
     3421 atggtgccaa tgcgctgggt actgtcggcg atatcgtcca ttgtcttgat caccgtggtc
     3481 atcgcctccc caccctgcac tgccgcatta ctggcggtga tagaaagttt atcggcagcc
     3541 gacgccgtgg cagagttctg tttcaccgac gccgccattt ggttcatggt cgccaccgtt
     3601 tgctgaacgt tatcaactgt ctgctgggta tgttcgttca gttcatcggt gcctttcgcc
     3661 agcgtctcac tgccatttct gacactggac acctggcttg agacatcgtt aattagccaa
     3721 cggcacatca ggccaagctg ccctaccgcg cgtaatgtca gccccagctc atcgctgcga
     3781 ttcaggtgct caacactatt acgctctccg gtcgccacct tcagtgcctg acgggcgaca
     3841 ttttctatcg ggcgcacaat ctgccattca aaacaagcgc ttgccaacaa taccactaac
     3901 acacagagga aatacgtcac caccggggca gcaacaaacc aaagcatggc cgccagcaag
     3961 ataaacatca gggtcatcac tccacgcgcc cgccagcgaa gcggtaatga aggcagttta
     4021 cccagccagc ctttacgcac caccaggcct ttatgaatac gcttactggt acgtccggcg
     4081 ttcagcgctt tgtacagcgg ctccaccgcc gcaatctctt catccgtcgc ccgggtacga
     4141 atcgacatat agccactgat ttttccctcg cgcaccatcg gtaccgcatt ggcccgcacc
     4201 caataatggt cgccattttt gcggcgattt ttcacgatgc cgctccaggg ctcccctttt
     4261 ttcagggtga accacatatc cgcaaacgcc gcttttggca tatccgggtg acgcaccatg
     4321 ttgtgcggct gcccttgtaa ctcttgcaag gtaaagccgc tcacctgcac aaaagtgtca
     4381 ttagcatgag tgatatagct ttgcagatcg gtagtggaca tcagagtggt atcgtccgcc
     4441 agcggggtat tttgctgggt gacatacgga tgagaagaca tgttcgcgtc ctgtgcaggt
     4501 tatatggttg ttaacttctt gtcagagttt atgtcggccc cgctgcggtt atctttaacc
     4561 gattaatttg atttagatcg caatttgcga ttaaagcaca aatctaattc tttgatttaa
     4621 aatactttca ctctgttact atacgaaaac gttaattatc ttgcccaaaa atcaggcaat
     4681 tattgccctg aaaacgtgca tttgcgcagc aatcatcaaa tccatacccg acaaaaaccg
     4741 tgcaaaataa caacaaatgt taacagatag cattaaatat tgcacaaatg ataaccgaat
     4801 ttgtgtttat cccgattttc gcgatcgcag ccggagtggc gcaatccctg caatacttaa
     4861 atcggtatca tgtgatacgc gagcctccgg agcatatttt gaacaggtta ccttcgagcg
     4921 catcggcttt agcgtgcagc gcccacgccc tgaatctcat tgagaagcga acgctggatc
     4981 atgaggagat gaaagcactt aaccgagagg tgattgaata cttcaaagag catgtcaatc
     5041 cggggttttt agagtatcgc aaatctgtta ccgccggcgg ggattacgga gccgtagagt
     5101 ggcaagcggg aagtttaaat acgcttgtcg acacccaggg acaggagttt atcgactgcc
     5161 tgggaggttt tggaattttc aacgtggggc accgtaatcc agttgtggtt tccgccgtac
     5221 agaatcaact tgcgaaacaa ccgctgcaca gccaggagct gctcgatccg ttacgggcga
     5281 tgttggcgaa aacccttgct gcgctaacgc ccggtaaact gaaatacagc ttcttctgta
     5341 atagcggcac cgagtccgtt gaagcagcgc tgaagctggc gaaagcttac cagtcaccgc
     5401 gcggcaagtt tacttttatt gccaccagcg gcgcgttcca cggtaaatca cttggcgcgc
     5461 tgtcggccac ggcgaaatcg accttccgca aaccgtttat gcagttactg ccgggcttcc
     5521 gtcatgtgcc gtttggcaat atcgaagcca tgcgcacggc tcttaacgag tgcaaaaaaa
     5581 ccggtgatga tgtggctgcg gtgatcctcg aaccgattca gggtgaaggt ggcgtaattc
     5641 tgccgccgcc gggctatctc accgccgtac gtaagctatg cgatgagttc ggcgcactga
     5701 tgatcctcga tgaagtacaa acgggcatgg ggcgcacggg caagatgttc gcctgcgagc
     5761 atgagaacgt acagccggat atcctctgcc ttgccaaagc gctcggcggc ggcgtgatgc
     5821 cgattggcgc gaccatcgcc actgaagagg tgttttcagt tctgttcgac aacccattcc
     5881 tgcataccac cacctttggc ggcaacccgc tggcctgtgc ggcggcgctg gcgaccatca
     5941 atgtgttgct ggagcagaac ttaccggctc aggctgatca aaaaggcgat atgttgctgg
     6001 acggtttccg tcaactggcg cgggaatatc ccgatctggt acaggaagcg cgtggtaaag
     6061 ggatgttgat ggcgattgag tttgttgata acgaaatcgg ctataacttt gccagcgaga
     6121 tgttccgcca gcgcgtactg gtggccggaa cgctcaataa cgccaaaacg atccgcattg
     6181 aaccgccact gacactgacc attgaacagt gtgaactggt gatcaaagcg gcgcgtaagg
     6241 cgctggcggc catgcgagta agtgtcgaag aagcgtaata tcaaatcgga tggcgatacg
     6301 acgtcgccat ccgatctttt tatacgacgc gtacgcccgc aggcatcatc cgctccggcg
     6361 ttaacagcac gctttcactg ccgtcatcgg tttccgcaca caacagcatg cattccgacg
     6421 tttcaccacg cattttcgct ttttgcagat tgcataagac caccaccgtt tttcccatca
     6481 gctcctcttc gctgtagtac ggcaccaggc tggtcacggt ttgcagtgtt ttttccccca
     6541 catcaacctg tacgatgtac agcttgtcgg cgttttcatg gcgtttcact tccacaatct
     6601 ttccgacgcg catttccaga cgtgcaaaat cagcgtaagc cacggtttcc attgcttcct
     6661 cccttcaagt aaaattttac taaactatag caaaattttt tctcaatcct gtaggctaaa
     6721 aatggagaat gcaggcgtga tcacattcgt aagccgctgt gttaccgtta cagcgtcaaa
     6781 gaaacgcgct ttatttactg aaaacaggtg acccgataag cacttcctct acaatggggg
     6841 cgcacatcag ggaaagtaaa aaaggtaaac atggcaacat taaaagacat cgcaatcgaa
     6901 gctggcgtat ccctggcgac agtatccagg gtcttaaatg acgatccgac attgaatgtg
     6961 aaagaagaga cgaaacatcg cattctcgag atcgccgaaa agctggagta caagaccagt
     7021 agcgcccgta aactacagac aggcgcagtc aaccaacacc atattctggc tatctacagc
     7081 taccagcagg agctggagat caacgatcct tactatctgg cgatccgcca cggcattgaa
     7141 actcagtgcg aaaagctggc gatcgaactg accaactgtt atgaacacaa cggattacca
     7201 gacattaaaa acgtcaccgg tattttaatt gtcggcaaac ccacgcccgc cctgcgcgcc
     7261 gctgccagcg cgttgaccga caatatctgt tttatcgact ttcacgaacc cggcagcggt
     7321 tacgatgcgg tggatatcga tctggcacgc atcagtaaag aaatcatcga cttctatatc
     7381 aaccagggcg ttaatcgtat tggttttatt ggcggtgaag atgagcctgg caaggcggat
     7441 attcgtgagg tcgcctttgc ggaatatggc cgactgaaac aagtggtacg cgaagaggat
     7501 atctggcgcg gcggtttttc cagttcgtcg ggttatgaac tggcaaaaca aatgctggcg
     7561 cgggaagact atccgaaggc actgtttgtt gcttccgatt ccattgctat cggcgtactg
     7621 cgggcaattc atgaacgtgg cctgaacatc ccacaggata tttcgctttt cagcgttaac
     7681 gatatcccca ccgcgcgatt tacctttccg ccgctctcca ccgtgcgcat ccattccgag
     7741 atgatgggaa gtcagggcgt taacctggtg tatgaaaaag cccgcgatgg tcgcgcgctg
     7801 ccgctgctag tcttcgttcc cagcaaatta aaactgcgcg gcacgacccg ttaaatcccc
     7861 ttacacactg tccggcaatc atttttgccg gacag
//
LOCUS       DAJVRF010000165         7890 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJVRF010000165 DAJVRF010000000
VERSION     DAJVRF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.165"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            78..2393
                     /gene="bisC"
                     /locus_tag="OQN13_003501"
     CDS             78..2393
                     /gene="bisC"
                     /locus_tag="OQN13_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCS1616770.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFTRRSAVLSSMQGSLPGGCDAVDKIPVARI
                     VEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISEC
                     FWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAEL
                     SERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQLIEMPEN
                     PDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEPDEWQGN
                     AEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRV
                     WNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDLPSSRLG
                     NGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2362..2802)
                     /locus_tag="OQN13_003502"
     CDS             complement(2362..2802)
                     /locus_tag="OQN13_003502"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1616771.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2799..3362)
                     /gene="tag"
                     /locus_tag="OQN13_003503"
     CDS             complement(2799..3362)
                     /gene="tag"
                     /locus_tag="OQN13_003503"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCS1616772.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3520..4218
                     /locus_tag="OQN13_003504"
     CDS             3520..4218
                     /locus_tag="OQN13_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1616773.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4447..5655
                     /locus_tag="OQN13_003505"
     CDS             4447..5655
                     /locus_tag="OQN13_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1616774.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFCVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5979..7670
                     /gene="eptB"
                     /locus_tag="OQN13_003506"
     CDS             5979..7670
                     /gene="eptB"
                     /locus_tag="OQN13_003506"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCS1616775.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDETYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7762..7838
                     /locus_tag="OQN13_003507"
     tRNA            7762..7838
                     /locus_tag="OQN13_003507"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7796..7798,aa:Pro,seq:cgg)
BASE COUNT         1810 a         1937 c         2089 g         2054 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttacgc ggcgttctgc ggtgctctct tccatgcagg
     1201 gcagcttgcc gggtggctgc gatgcggtgg ataaaatccc tgttgcccgc attgttgaag
     1261 cactggaaaa ccctggtggc gcatatcaac acaacggtat gaaccgacat ttcccggata
     1321 ttcgttttat ctggtgggcg ggcggtgcca actttactca tcatcaggat accaatcgtc
     1381 tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc tgaatgcttc tggacggcgg
     1441 cggcgaaaca cgcggatatc gttctgcctg cgactacctc ttttgagcgt aatgatctca
     1501 ccatgaccgg tgattacagt aatcagcatc tggtgccgat gaagcaagtg gtgccgccac
     1561 gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt gagtgaacgt tgggagaagg
     1621 gcggctatgc gcggtttacg gaaggaaaaa gtcagctgca atggctggaa acgttttata
     1681 acgttgcccg gcagcgcggg gcaagccagc aggttgaatt gccgccattt gctgagttct
     1741 ggcaagccaa ccagttaatt gagatgccgg aaaacccgga cagcgagcgg tttattcgct
     1801 tcgctgattt ttgccgcgat ccgctggcgc atccgttaaa aaccgccagc ggcaagattg
     1861 aaatcttctc acagcgtatt gccgattacg gttacccgga ttgccctggg catccaatgt
     1921 ggctggagcc ggacgaatgg cagggcaatg ccgaaccaga acagttgcag gtactttctg
     1981 cccatccggc gcaccgcctg cacagccagc tgaattacag ttctctgcgc gaattgtacg
     2041 cggtagcaaa ccgtgagcct gtcaccattc atcctgacga tgctcaggcg cgcggcataa
     2101 ccgaagggga tatggtgcgg gtgtggaact cccgtgggca gatccttgcc ggagcggtca
     2161 ttagcgaggg aattaaacct ggcgtgattt gcattcacga aggggcatgg ccggatctgg
     2221 atttaaccgc tgacggtatt tgtaaaaacg gcgcagtgaa cgtgctgacc aaagatctcc
     2281 ccagctcgcg gctggggaat ggctgtgcgg gtaatacggc gctggcatgg ctggaaaaat
     2341 acaacggtcc ggaactgaca cttacagcgt ttgaaccacc ggccagctca taatccaggt
     2401 gggtagttgg gtttcatcct gccatgcgca atcgacaatg tgaaaaccct gcgcacggta
     2461 aaaatctatc gccggttgat ttttttgata aacctccagc atcagatggg gatagcgctg
     2521 ctgcacatac tgcatcagcg ccttaccaat accgcgtctg acggccttcg gtgcgacaaa
     2581 cattgctgcc agaaatcggc cttccataat gctgacaaaa ccgagaagct taccgtcctc
     2641 ttcccagacc cagttttgcg cgttggcaag ataggcatcc cgcaccagcg gaatgcagtc
     2701 acgccagtaa ttcgctttta taaagggatg cccccaggtt gtactttcca gccacagttc
     2761 gaggatcgcg gggagttctg aacgttgcgc ttcccgaatc atggtttatt tcccggatag
     2821 caacagcagc caaccacatg atcattcacc agcccacatg cctgcataaa ggagtaacag
     2881 attgtggtgc cgacaaactt aaaaccacgt tttttcagtg ctttagatag ggcgtcggag
     2941 gcggacgtag atgtgggaat ttcgctcaac gttgtggctt gtgtcacctg tggctgatga
     3001 tttacaaacg accagacaaa gtcggcaaac ggttcgccgt tctgttccat ttgcaggtac
     3061 gcccgcgcat taccaataat tgcctgaatt ttccctcgat ggcggataat cccggcgtcc
     3121 tgtaccagtc tttcgacatc ctcttcctgc attgctgcga ccttcaccgg atcgaactga
     3181 tgaaagcagg cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga taacccagcc
     3241 tgctgccctt caaggcagat catttcgaac agttttttac tgtcagtttc aggcacgccc
     3301 cattcattat catggtaggc aatataaagc gggtcctgac tcacccagcc gcaacgttcc
     3361 atactttccc tcgctatgta cccgatcgta aaaatttcag ccgcttttct tgacgcatcg
     3421 gtgaaaaagt caatatttaa tacagggcta ctatgcccga tatccttcat acaatgacaa
     3481 taatattgcc gaattcggct cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt
     3541 ggtcgctggc agctaagcct gctggcgagc gtggtaatca gtgccttttt tctcaacaca
     3601 gcttacgcct ggcaacaaga atatatcgtt gatacgcaac ccggacattc cacagagcgt
     3661 tacacctggg atagtgatca tcaacctaat tacaacgata ttttgtcgca acgtattcaa
     3721 agtagccaaa gggcgctggg actggaagtc aatctggcgg aagaaactcc tgtggatgtg
     3781 accagcagta tgagtatggg ctggaatttt cctttatatg aacaggttac aaccggcccg
     3841 gtcgcggcat tacattacga tggcacaacc acctcgatgt ataacgagtt tggcgacagt
     3901 actaccacgc agaccgatcc gttatggcat gccagcgtga gtaccttagg ctggcgtgtt
     3961 gactcccggc ttggcgatct ccgaccctgg gcgcaaatca gctataacca gcaatttggc
     4021 gagaatatct ggaaggcgca atcaggcctg agccggatga cggcgacaaa ccagaacggc
     4081 aactggctgg atgtcaccgt aggcgctgat atgttgctca atcaaaatat tgccgcctat
     4141 gccgcgctaa ctcaggcaga aaataccact aataatagcg actatctgta tacgatgggg
     4201 gttagcgcca gattttaacg taacagtcac aattgaaacc attaaataac aataattgtg
     4261 gcgatagtgg gtgctaactt accaaataat aaatttggtg aataattgtc gcgtcattca
     4321 ttcctgaact aaggcatttc attccgttct gatggcattt catgccgttt ttccccaggc
     4381 ataaagtgca cttcgttatg gttgtcggca gagatttttc ctttttatta ctgcaggaat
     4441 actgccatga caccttcaaa ttatcagcgt acccgctggc tgacactcat cggtactatc
     4501 attacccagt ttgcgctggg gtcggtttat acctggagcc tgtttaatgg cgcgctttcc
     4561 gccaagctgg atgcgccggt aagccaggtc gctttctctt tcggcttgtt aagtctgggg
     4621 ctggcaattt cgtcttctgt tgcgggcaaa ttacaggaac gtttttgcgt taaacgcgtc
     4681 accatggctt ccggcatttt gctgggatta ggcttcttcc tgaccgcgca ttccaacaac
     4741 ctgatgatgc tgtggttaag cgccggtgtg ctggtcggtc tggcggatgg cgcgggttac
     4801 ctgctgacgc tctctaactg cgtgaagtgg ttcccggagc gtaaaggtct gatctccgcg
     4861 ttcgctatcg gttcttatgg tctgggtagc ctgggtttca aatttatcga cacgcagctg
     4921 ctcgaaacgg tgggtctgga aaaaacattt gtgatttggg gagcgattgc gctggtgatg
     4981 attgttttcg gcgcaacgtt aatgaaagac gcaccaaaac aggaagtgaa aaccagcaat
     5041 ggtgtggtgg agaaagatta cacgctggca gagtcgatgc gtaaaccgca gtactggatg
     5101 ttagcggtaa tgttcctgac tgcctgcatg agcggcctgt acgtgattgg ggtagcgaaa
     5161 gatatcgccc aaagtctggc acaccttgat gtggtttccg cagccaatgc agtaacggtt
     5221 atttccatcg ctaacctttc aggtcgtctg gtgctgggca ttctgtctga caaaattgcc
     5281 cgtatccgtg ttatcaccat tggtcaggtg atttcgctgg tgggtatggc ggccctgctg
     5341 tttgcaccat taaatgcagt gacgttcttt gcagcgattg cctgcgtggc atttaacttt
     5401 ggcggcacta ttaccgtctt tccatcactg gtcagtgagt tcttcggcct caataacctg
     5461 gcgaaaaact acggtgtgat ttacctcggt ttcggtatcg gtagcatttt tggttcgatt
     5521 atcgcctcac tgtttggcgg tttctatgtg accttctacg tgattttcgc cctgctgatt
     5581 ctgtcattgg cgctttctac gacgattcgt cagccagagc agaaaatgtt gcgtgaggcg
     5641 catggctccc tttaatgctc agccgcgagc tactgggctt cagcttccgt gtgcatcaaa
     5701 cgcatcaacg aaatgtataa acaatgactc gtaaaatttg ggccagttaa ctggcccttt
     5761 ttactgcttt ttgtaacgct aaagtctctt ttcaaacttg catttttgta aatttgtgct
     5821 tcatgcacac tctttcccca cactttttcg ctttgctgtg gtctacttat tcgcgcgtgt
     5881 agattttact tatctgacta cctccgcact tttcccctgc cgggcctgaa aagccgttaa
     5941 gcagggtgtt atcacctgtt tgtccagggt ttgtttgcat gagatacatc aaatcgatta
     6001 cacagcagaa gctgagcttt ttgcttgcaa tctatattgg cctttttatg aatggcgcgg
     6061 ttttttatcg ccgcttcggc agctatgcgc acgattttac cgtctggaaa ggcgtttctg
     6121 ctgttgttga actggccgcc accgtactgg tgaccttctt tttactacgt cttctatcgc
     6181 tgtttggccg ccgcagctgg cgtattctgg cgtcgctggt ggtgctcttt tccgcaggtg
     6241 ccagctatta catgaccttc cttaatgtgg tcattggtta tggcatcatc gcttccgtca
     6301 tgaccaccga tatcgacctg tcaaaagaag ttgttggtct gaactttatt ctctggttaa
     6361 tcgccgttag tgcattgcct cttatcctta tctggaataa ccgctgtcgc tacaccttgc
     6421 tccgacaact gcgaacccca gggcagcgta ttcgcagcct ggcggtcgtc gtactggcgg
     6481 gtattatggt ttgggcaccg attcgtttgc tggatatcca gcagaagaaa gtggagaggg
     6541 cgaccggcgt tgatttgccg agttatggcg gtgtcgtagc gaactcttat ctgccatcaa
     6601 actggctttc tgcgttgggg ctgtatgcct gggcgcgggt cgatgaatct tccgataata
     6661 attcattgct taatccggcg aagaagttta cataccaggc tccacaaaac gttgatgaga
     6721 cttatgtcgt gtttatcatc ggtgaaacca cgcgttggga ccatatgggt attttcggct
     6781 atgagcgtaa taccacgccg aaactggccc aggagaaaaa tctggcggcg ttccgtggtt
     6841 actcctgtga taccgcaacc aaactctcac tgcgttgcat gtttgtacgt caggggggcg
     6901 cggaagataa tccgcagcgc acattaaaag aacagaacat tttcgcggtt ctgaagcagt
     6961 taggattcag ttctgacctc tacgctatgc agagcgaaat gtggttctac agcaacacga
     7021 tggcggacaa cattgcttat cgtgagcaga ttggtgcgga gccacgtaat cgtggcaagc
     7081 cggtagatga tatgttgctg gtagacgaaa tgcagcaatc gctagggcgc aacccggatg
     7141 gtaagcatct gatcattctg cataccaaag gttcgcattt taactacacc cagcgttatc
     7201 cgcgtagctt cgcgcagtgg aagccggaat gtattggtgt tgatagcggc tgtaccaaag
     7261 cgcagatgat caactcctat gacaactcgg tgacctatgt ggatcacttt atctccagcg
     7321 tgattgatca ggttcgcgat aagaaagcga ttgtgttcta cgcagctgac cacggtgagt
     7381 caattaatga acgcgagcac ctgcacggca cgccgcgtga actggcaccg ccggagcagt
     7441 tccgcgtacc gatgatggtc tggatgtcag ataaatatct ggaaaatccg gccaatgcgc
     7501 aggcgtttgc gcagctgaaa aaagaagccg acatgaaagt gccacgccgt cacgtagagc
     7561 tgtacgacac catcatgggt tgtcttggct atacttcacc ggatggtgga attaacgaaa
     7621 acaacaactg gtgtcacatc ccgcagacaa aagaggcagc ggctaactaa gaggcctgct
     7681 gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg cgtatctgcg
     7741 cagtaagatg cgccccgcat tcggtgattg gcgcagcttg gtagcgcact tcgttcggga
     7801 cgaaggggtc ggaggttcga atcctctatc accgaccaaa ttcgaaaagc ctgctcaacg
     7861 agcaggcttt tttgcatttg tagctcatga
//
LOCUS       DAJVRF010000166         7847 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJVRF010000166 DAJVRF010000000
VERSION     DAJVRF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.166"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(101..796)
                     /locus_tag="OQN13_003508"
     CDS             complement(101..796)
                     /locus_tag="OQN13_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCS1616776.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(789..2216)
                     /locus_tag="OQN13_003509"
     CDS             complement(789..2216)
                     /locus_tag="OQN13_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCS1616777.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2227..2946)
                     /locus_tag="OQN13_003510"
     CDS             complement(2227..2946)
                     /locus_tag="OQN13_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCS1616778.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="OQN13_003511"
     CDS             complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="OQN13_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HCS1616779.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            4554..5879
                     /locus_tag="OQN13_003512"
     CDS             4554..5879
                     /locus_tag="OQN13_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HCS1616780.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            5988..6224
                     /locus_tag="OQN13_003513"
     CDS             5988..6224
                     /locus_tag="OQN13_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1616781.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6236..6829
                     /gene="rclC"
                     /locus_tag="OQN13_003514"
     CDS             6236..6829
                     /gene="rclC"
                     /locus_tag="OQN13_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HCS1616782.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(6989..>7847)
                     /locus_tag="OQN13_003515"
     CDS             complement(6989..>7847)
                     /locus_tag="OQN13_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCS1616783.1"
                     /translation="VLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLIDTAAV
                     YGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSGLEYF
                     DLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMVNQVE
                     LHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQVILRW
                     NIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNPEFVR
                     GCLAVKIHD"
BASE COUNT         2111 a         1932 c         1682 g         2122 t
ORIGIN      
        1 agcataacca tgacggaacg gggttaagtc aggttgtcct taacgctatt ttcaacctcg
       61 tatgccttct tcaggtttat gttcagatct catatctctc tcagcaatcc tcaataatca
      121 gatacaccgc tttcaccgga ccatgaaccc cgacgacttt gataagctca atatccgccg
      181 ttgagctggg accactgatg atgttaatac atgacggcat tcgttcaccg gcctgcgctt
      241 tctgatgcaa tttttctgcg agttgcgcta cacgcggcag gattgtgctt ttacgcagga
      301 taaaaagaga agattccggg agcaggctca atgaacgccc gcgctcgacg gcggaaaaaa
      361 gaaccacgcc tcccgattcg gttaaaccat attcagcata cacgacaccc actttagcct
      421 gctctgcctg cgagatattc tcggcacctt tcgccggatc ccaaacaacg gcattgcatt
      481 cctgctgcaa acgttcacta atccccaatt cctccagcct cgtgtcaccg ctaatcacga
      541 ccgactgatc tcccagctct ttacacagac gtattgcagc ttctgccgcc ttcgcctcgc
      601 tggtcagctc acagcgcgtc aacataacat cgctggcaaa ctgaataaac gcgtcacagc
      661 gctgctgttg gttaagttgg gtaagccgct cgttagcata gttgttaagc ggcgcatctt
      721 ctgcttgcgg ttcaagtcgc agcgggcgac ccagtgcctg agcaacgtta ttcaaaaatt
      781 cgctccgatt atccattctt tttctcctgc gcctgatgtt tcttaaacca actacggaaa
      841 ctctctccgt cagcttcagg aagatcgcgt gcttccatcc agtcgctaat cgcgccaaat
      901 ttgagtggtg ttttgccgcc attgataaac cagcttgccg catgagcgcc ggccatcatc
      961 ccgactttcc acaatcctgg atgactattg gcataagcga acatttttat cgcccgttgc
     1021 tctgcttttg cggtgatccc tttttcagcc atcacccgac gatgacgcaa aatcagtttt
     1081 gacagcggaa tacgcaccgg acacacgctg tcacaagcag tgcataaaga gcaggcgtag
     1141 ggtaaatctt taaaatcttt atagccgcca agtagcggag aaatcaccgc accaattggc
     1201 cctggataaa tagagccata tccatgaccg ccaatatggc gatatgccgg acaagtattc
     1261 atacaagccc cgcagcgaat acagcgcagc acatcccgaa attcagaggc cagcacctca
     1321 gaacgcccgt tatcgacaat aaccagatga aactcttcag gaccatcaac gtgccccgct
     1381 tcgcgcggtc ctgtcagcca ggtgttgtat cccgtcaaac gtgcaccaac ggcactgcgc
     1441 gccagcatgg tgatcaatac atctacctcg gcaaacgtgg gggcaatacg ctccattccc
     1501 atcacagcaa tatgcgtttt aggcagcgtg gtacacattc gcgcattacc ttcattggtc
     1561 accaggcata ccgaacctgt ctctgccacc gcgaaattac agccggtaat acctatttca
     1621 gcactgagga aatcttcgcg gattttttgc cggatgaata aggtcatcgc ttcaggcgtt
     1681 tccggcccct catagcccag acgttcgtgt agcactcgac ggatctgatg gcgatcttta
     1741 tgaattgccg ggaccacaac atgagatggc ggatcttgat ccagctggag aatatattca
     1801 cccagatcgg tttcaatcac ctgaatgcca gcatcctgca acacatgatt gacaccaatc
     1861 tcttcggtca ccatcgattt agatttcacc accttccggg catttttgcg ttgggcaacc
     1921 tgtaaaatgt agcgggtagc gtcttctttg gtttttgcaa accagacgtg accgccgttt
     1981 tgcgtcactt tttctgagag ctggtacaga taagcgtcga gattactcag aacatgatca
     2041 cgtatctggg cggcccgatc gcgccactcc tcccagtgcc ccaattcatc gaccattttt
     2101 tgccgatttg ccccaatacg ctgctgcgcg tttgccaccg ctttgcgcat gatcggatct
     2161 tcaatttgct gacggatgcg tgtcttaaaa tctgtattac tggttttgat cgacatcttt
     2221 atatcctcag cggctcatca acacttcagc aatatgcatc actttgactt tctgcccttc
     2281 ccgttgtaat cgcccactga tgttcagcag gcaactcacg tcagcaccaa ttaaatactc
     2341 agggcggact tccatcaggt gcacaacctt ttctttcacc atctcgccgg atatttcggc
     2401 cattttgacc gagaacgtgc cgccaaatcc gcagcaggta tcctgttcag caaaggtcaa
     2461 cagctccagt ccacgcacat ttttcagcag cgtaagtggc tcgtccttca ctcccagctt
     2521 acgggccagg ctacaagatg ggtgatacac agctctccct tgcaaactgg cacctacatc
     2581 gactacccct aatttattaa caataaaaga ggtgagatcc tgcatacgcg cggcaacctt
     2641 ttcggcacgt gatgcccatt caggttcatc cgccagatac gtcgggtaac tttttacggc
     2701 ataggtgcaa gagccagccg gtgaaataat gggatcgtcg ttatcctcca gtgcggcgat
     2761 cagatttttc atccctggaa tcgcttcttt gatataaccg ctattgatcg caggctgacc
     2821 gcagcatccc tgtttctccg ggaaatttac gcgacagccg agtttttcca gtagcagcac
     2881 ggagtctcgt gccattcttg atttcagggc gtcaccaata caggtgacaa agaaattgac
     2941 attcacaact aatactccat tacttcatgc ccatttatgc ccgatggttc ccctccttcc
     3001 gggggatcaa tcatcaataa atatggcgtt cttttttaat tggctagcaa cataccaccc
     3061 ctcatttgtg tgacaaatga taatctttca tccaaacttc aaatgcaata actaatttta
     3121 tttctttcgg catcgctcac attttatggg gaaatcgacg ttcatttttg gcataagaaa
     3181 tccatttaaa aaggctacat cccttatacc tattcagagc tacttattca ggggttattg
     3241 agatatacac tgatggtgta caaaaaaatc accgacaata atcacgtatc aagtaatagt
     3301 cggggtttta tttaattgaa gcaatttagt ttgcattttt atttaaaaac tgagctagag
     3361 tccatcagaa aaaaatatat aaagcccaaa ccaccgttgg aaatagaaat agttgaaagg
     3421 ctcacaactc ccggaatgcg gattatcttt gccctgattt ctggcggttt tattcagggt
     3481 gcaagctgtc tgaccctttc ccgatattct cccggagtac aaccaaactc gcggacaaac
     3541 gccttgtgaa aagatgattc actggcatag cctactgact cagcgatcac cacaacaggg
     3601 agcatttccc gggaaaacat ctgggccgct atttgtagac gcaactttgt taatacagcc
     3661 agcggcgtgg ttccggaaac atcacggaaa agctgggcaa aacttgcccg agacatgtgg
     3721 gcgatgctgg ccagcgattc gacggtccag gcgtgtcctg gcatttccag catttgctgt
     3781 attaccgcac caagacgtgg atgcagaagc aaactgagaa tgtttttctc tgtattaacc
     3841 tgtgcaatcc actcacgcac cgcgagggta aagaatgtag cgcagatctg gctacacagt
     3901 gcatccactc ccggcatcgc cgatctggat tcctgttgta aaaacgggat cgcctccctc
     3961 agccagttat attccacgct gtggttaacc ggtgctaaaa acagcgtttc cggcaaagac
     4021 gttaaaaaat aacgcgccga atgttgcaac cgaagagtgc cgcagacaat acaggtcgat
     4081 tcattatcga catgactcag gcgatgagct gaattttgtg gtagcaggac cacatttccc
     4141 gggcgtaatg taaaaatctc tcccgtcggc atttccagct tcgccgctcc ttgcgttaac
     4201 gcatgccaac gaataaccga taattccccg gcaccatgtt gaagctgcca gtcacttcct
     4261 aacacgcaat tcttatcgat cgttccttgt ggagcgttaa gcatcaacaa acggctaagg
     4321 gcatccatat cagactcctg agtggttttt gattacaaac accaacaatc ttagtaaata
     4381 actaatctaa taccatcaaa tggttaatca tgatttttgt tttgtgattc tgattaagaa
     4441 aaagaaaata ccagacgata agacaaaata tcaagcgttg tgaagaaatg gtatttgctc
     4501 ttttgcgtct atagtcatga tgtcaaatga acgtgtttcg acaggaaatc atcatgaata
     4561 aatatcaggc agttattatt ggttttggca aggctggaaa aacattagcc gtcacgctgg
     4621 caaaagcagg ttggcgagtg gctctcatcg aacaatcaaa tgcaatgtat ggcgggacct
     4681 gtattaatat cggctgcatc ccaaccaaaa cattggttca tgacgcacag cagcacacag
     4741 attttgtccg tgccatacag cgtaaaaatg aagtggttaa ttttttacgt aataagaatt
     4801 ttcataatct tgcggatatg cccaatatcg acgtgatcga cggccaggcg gagtttatca
     4861 ataatcatag cctgcgtgtt catcggcctg agggaaatct ggaaattcat ggcgagaaaa
     4921 tttttattaa taccggtgca caaaccgtgg ttccgccaat tcctggaatt accaccacgc
     4981 ctggtgtata tgacagcacc ggattactta atctaaaaga attgcctggg catttaggta
     5041 ttttgggcgg cggatatatt ggcgttgagt tcgcctctat gttcgctaat tttggcagca
     5101 aggtaaccat tttagaagca gcttcgctgt ttttgcctcg ggaagatcgg gatattgctg
     5161 ataatatcgc gacgatttta cgcgatcagg gcgtcgatat tatcctcaat gcccatgtgg
     5221 agcgaatcag tcaccatgaa aatcaagtgc aagtgcatag cgagcacgcc caactggcgg
     5281 tggatgcact gttaatagct tccggtcgtc aaccggctac cgcttcgtta catccagaaa
     5341 atgccggtat cgcagtaaac gagcgcgggg caattgtcgt tgacaagcaa ttacatacca
     5401 ccgcagacaa tatttgggcg atgggagatg ttaccggcgg gctgcaattt acttatattt
     5461 cactggatga ttaccgcatt gtgcgtgatg agttgctggg agaaggcaga cgtagtactg
     5521 atgatcggaa aaatgtgcct tattccgtat tcatgacacc gccactgtcc agggttggta
     5581 tgacagaaga acaggccaga gagagtggtg ctgatattca ggtggtgaca ttgcctgtag
     5641 ctgcaattcc gcgtgccaga gtgatgaatg atactcgtgg ggtattaaaa gcgattgttg
     5701 ataataaaac ccaacgtata ttaggggcat cactgctgtg tgttgactcc cacgagatga
     5761 tcaatatagt gaaaatggtg atggatgccg ggctgcctta tagcatatta cgcgatcaga
     5821 tatttactca tccgtcgatg agcgaatcac tcaatgatct attttcatta gtcaaataaa
     5881 ctcaaaatca gacgccagaa caaatattct ggcgtctcag agaaaagaat cttattaatt
     5941 ctctgttact ctataacttc ttaatcactt cattgatggt attttatatg tttaaaaaat
     6001 ctgttttatt tgcaacacta ttatctggcg ttatggaatt ttccaccaat gcagatgata
     6061 aaataattct gaaacatatc agcgtctcgt cagtatcagc atcaccgaca gttctggagg
     6121 ataccattgc tgatatagcc agaaaatata atgcttcatc ctggaaagtc acatcgatgc
     6181 gaattgataa taattcaacc gcaacagcag tattgtataa ataaggatgt tcacaatgga
     6241 aaaatacctg cacctgttaa gtcggggaga taaaattggc ctgtcattga ttcgtctgag
     6301 tattgcgatt gtttttatgt ggattgggtt attaaagttt gtcccttacg aggcagacag
     6361 cattacacca ttcgtcgcaa acagtccact aatgtcgttc ttttatgaac acccggaaga
     6421 ctataaacag tatctgactc acgaaggcga atacaaacca gaagcaaggg catggcaatc
     6481 ggccaataat acctatggtt tttccaacgg tcttggcgtc gtggaggtga ttattgctct
     6541 gctggttttg gctaatcctg tcaatcgctg gttaggttta ttgggaggac tgatggcata
     6601 taccacgccg ctggtaacac tctcattttt aatcaccacc ccggaggcat gggtacccgc
     6661 attgggtgac gctcatcatg gtttccctta tttatccggt gctggtcgct tggtattgaa
     6721 agatactctg atgctggcag gtgctgtcat gataatggca gattcggcgc gggaaattct
     6781 taaacaacgc agtaatgaat ccagttcaac gttaaaaact gaatattgat aatccgaaca
     6841 ctgtctgttg ttcacttttt caggcgtcag gccccctttc tgatgatgaa gcctgacgcc
     6901 atattaccgg agatatgaca gagtgaagat caatatcact cccctgttaa aagattcttg
     6961 tcgtctcagg taaaatatat gattaatatc aatcatgtat ttttacagca agacaaccac
     7021 gaacaaattc aggattaaaa tgttttaccg actccccaac ataacccaaa tcaagcgaac
     7081 taatttgtgc catttctttc tctgtcagtg agaaatccca gatagcaaaa ttttcttcga
     7141 tacgctgttg atgtgtcgat ttcggaataa cgaccactcc ccgttgaata ttccaacgca
     7201 gaatgacttg agaaatcgat ttttgatggg catctgcaat actctgaagc agattatttt
     7261 caaagggttt atgtcgtcca ccacctaacg gagcccatgc ttcaggctgc acgttataat
     7321 acttcatggt tgccagcgcc tctggttggg caaaataggg atgcaactcg acctggttga
     7381 ccatcggttt aactcttacc gtttcacaaa agttcgccag aacatgagga tagaaattgg
     7441 aaaccccaat tgcttttaat ttgccctctt cataggcatc ttcgagtgca cgccacacac
     7501 tgaaataatc gcgcatagcc tggtgcaata aatagaggtc gaagtactct agtcccgatt
     7561 tttttaatga tgcttcaata cctgctgctg ccgtatcctg attcaacata tcctgccccc
     7621 acaatttaga cgttataaat aactcatctc gggtacataa accttcagat atagcttcac
     7681 gaacggcttc accgacggca tcttcattac cgtataccgc tgcagtgtcg atgagtcgat
     7741 aacccgtgcg gatagcgttc aacactgact gtttgcacac gtttttatcg gtaacctgaa
     7801 aaacaccaaa tcccatcatc ggcattttca gattgttact taatacg
//
LOCUS       DAJVRF010000167         7843 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJVRF010000167 DAJVRF010000000
VERSION     DAJVRF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7843
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.167"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..135)
                     /locus_tag="OQN13_003516"
     CDS             complement(<1..135)
                     /locus_tag="OQN13_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCS1616784.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     L"
     gene            complement(252..1298)
                     /gene="ansB"
                     /locus_tag="OQN13_003517"
     CDS             complement(252..1298)
                     /gene="ansB"
                     /locus_tag="OQN13_003517"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCS1616785.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(1474..2193)
                     /locus_tag="OQN13_003518"
     CDS             complement(1474..2193)
                     /locus_tag="OQN13_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCS1616786.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(2377..2703)
                     /locus_tag="OQN13_003519"
     CDS             complement(2377..2703)
                     /locus_tag="OQN13_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCS1616787.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(2703..3422)
                     /gene="trmB"
                     /locus_tag="OQN13_003520"
     CDS             complement(2703..3422)
                     /gene="trmB"
                     /locus_tag="OQN13_003520"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCS1616788.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            3583..4635
                     /gene="mutY"
                     /locus_tag="OQN13_003521"
     CDS             3583..4635
                     /gene="mutY"
                     /locus_tag="OQN13_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCS1616789.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKTKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4663..4938
                     /locus_tag="OQN13_003522"
     CDS             4663..4938
                     /locus_tag="OQN13_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCS1616790.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            5003..6082
                     /gene="mltC"
                     /locus_tag="OQN13_003523"
     CDS             5003..6082
                     /gene="mltC"
                     /locus_tag="OQN13_003523"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCS1616791.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLDGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            6284..7540
                     /gene="nupG"
                     /locus_tag="OQN13_003524"
     CDS             6284..7540
                     /gene="nupG"
                     /locus_tag="OQN13_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCS1616792.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7589..>7843)
                     /locus_tag="OQN13_003525"
     CDS             complement(7589..>7843)
                     /locus_tag="OQN13_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCS1616793.1"
                     /translation="IRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQR
                     YFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
BASE COUNT         1847 a         2077 c         1919 g         2000 t
ORIGIN      
        1 aagcttgaga atgccgtgat actgcttttt gaattgatct tttaagatat tttcagttgg
       61 cggttcgcca tctgcccagg cattaccagt catcaacata agcagtgccg tgccaacaat
      121 ccattgcttt ttcacccgaa aaacctcacg cggaataatt tattccggtg attatcatta
      181 gggtaatgaa aattggcaat ggacgtaagt ctgaagtgaa aaagccccgg cacgataccg
      241 gggcgaggtg attagtactg attgaagatc tgctggatct gctgcggatc tttggtttgc
      301 gtcagagcca gctgcagcag gacgcgcgct ttttgcgggt tcagcgtgcc agaggcgacg
      361 aagccgtatt tcgcatcatc cacttcagca tcctgagtgg tagcacccgt cggtacgcgg
      421 gaagaacgca ctactgcagt gccgtttttc gcggcggttg ccagggtgtc gaacacggat
      481 ttatacaggt taccattacc cacaccagcg ctaacgatgc catcatagcc cgcatctacc
      541 agtgctttag ccggaagatc ggatgcgtta gcgtagttat aaacgatgcc gactttcggc
      601 agctcattca gcttagagac atcgaatggc gtatcgctgg tgtgcttacg tgccggggta
      661 cgttggtagt caatcttacc gttgtgaatg tatcccagag gaccgtagtt aacagacttg
      721 aaggtcgcta cgtcggtggt gttggttttg gtgacatcgc gaccgtccag tacggtgtcg
      781 ttcatcacca ccagcacgcc acgattagcg gaggctttat cagctgcggt cactaccgcg
      841 ttatacaggt tgaatggacc gtctgcgctc atggacgtgg acgggcgcat tgcgccgacc
      901 atcaccaccg gtttgtcgca tttcaccgtc aggtcgagga agtaagcggt ttcttccatc
      961 gtgtcggtac cgtgggtaat gacgaagccg tcggttttat cgcagtcggt gttaattttt
     1021 ttcgccagtg tcagccagac attatcgttc atgtcctggg agccgatatt cactacctgc
     1081 tcgcctttaa cgttcgcaat gtcttttagt tgcggcaccg cattaaccag attttctacg
     1141 ccaactttac ccgctgtgta gttagatttg gttgcggagt caccaccacc ggcaatggtc
     1201 ccgccggttg ctaaaatggt gatattgggt aatgccaatg ctgcaccact aaaacccata
     1261 accagtgcgg caagtgccgt ctttttgaaa aactccattt cattcctcca gttacgtgaa
     1321 cgctacgcat tatcccttag ctctgtatgg gaaatttgac gttaaacaat ttacaacgtg
     1381 aatatatttt ggagatctac aaagttagag gcaggtaaca aaacgaagaa ttaaacggca
     1441 taaaaaagta ttatgccgtc ttaaaataga ggattatttt aaattaccga ccagggcttt
     1501 gcggctatct tccagagtca caacgcggct acaaacatct ttgccaaact gctggaaatc
     1561 tttttcctgc tttttccact ctgtttggat tgaggattgc agccccccca ggcttcccag
     1621 cacattctgt aatgggttac cgccgctttt cagcaccgct ttcgcgccca tttcattaat
     1681 gctgtcctgt aaaattccac ccattgcctg attcactaat tgctggcctt cggcgcgaac
     1741 ctgatcaatg gctttatagt gaaacgtcag gccatcgctg cgcgtttcga taatgcggtt
     1801 catctgctct ttcagctgcg catcaagttt ggtcagacgg ctgcgcattt tgctgctttc
     1861 gcccatctcc tgaacgataa ttttatccag cgcaatacgg gctttttcga cgcggctttt
     1921 cgcgccttca tcaatccacg gcagcgtgct gcgtagttca gcctgataat ccttcgcctg
     1981 ctcgcgctgg gcggcattca gggaatattg cttaccgtta tacatcacgt tgccgtctgg
     2041 cgtgatcacc agattgccgt tttcgccctt cacctgcacg gtttgcgggc tgacaatcac
     2101 atcgtcacgc ggcgtgacgc tgcactggta gtcggcgtga gcggtcattg ccgtcactga
     2161 aagtgctgcc gccagcagca ttttgcgcat catagtcttc cctcaagaaa aaatcaggcc
     2221 agcatttgct ggccccagat tgataacaaa gtacgcgttt tccatgccgg atgcggcgta
     2281 aacaccttat cccgcctact cgatcgtgca tctttccagg cctgataagc gtagcgcatc
     2341 aggcagtttt gcatttgtca tcgcccgtat gctttcttag tcccaccaaa cgtcgaaaag
     2401 ttcgctggtg cgtacctcat ccagtttgcg ctcttccagc cacttacgca caatcgcctg
     2461 atgttcttcg gtgcatttgc cgatttcctg catgcagatc agaccttccc aggccagata
     2521 accgctgccg tcaaaggcca gtttgttcgg ttcgataacc tcgttaataa aatcatcaac
     2581 ggttttatca atctgttctt ccgatgtacc ttccgggaat cgccatgcca ccgaaaatcc
     2641 taattcctgg aattcgtcga tgtgcatttt tttacgcaga cgacggctac ggttctttgc
     2701 cattatttca ccctctcgaa cattaagtcc catactccgt gaccaagacg atgaccacgt
     2761 tgttcaaatt tcgtcaccgg acgtgatgcc ggacgcggca cgtaatcatt gctctctgac
     2821 aggtttttat aaccgtcaat agaagacatc acttcaagca tatgttccgc ataaggttcc
     2881 cagtcggtcg ccatatggaa tacacccccc agctgcagtt tgctttttac cagttcggca
     2941 aacggcacct gtacgatacg gcgtttatta tggcgcgctt tgtgccacgg gtcagggaaa
     3001 aagagctgca ccatgcgcaa tgaattgtca ggaatcattt tatgcagcac ttcaaccgca
     3061 tcgtgacaca tcacgcgcag gttgcttaaa ccttcttcat gcgcagaagc caggcacgca
     3121 ccaacgcccg gtgaatgcac ttcaatgccg aggaagtcct gctcagggcg atctttagcc
     3181 attgccacca gcgacgcccc catgccaaaa ccaatctcaa gcgtcaccgg cgcttcacgg
     3241 ccaaaaagcg cggggaaatc cagcatatct tcgctgaact caacgcccat caccggccag
     3301 tagttttcca gcgcatgttc ctggcctttg gtcagtcgcc cctggcggcg cacaaaacta
     3361 cggatacggc gcagtgggcg gccgttttca tcaaattccg gtgaaatgac gtcgtttttc
     3421 ataaagattt agtcgcttgt gaaagtgttc tgaaaacggg cattatccaa agttagttgc
     3481 cggatgcaag catgataagg ccgtggctgc ggaaagttcc ggtttacacc ctgccgtcgc
     3541 tgtgctgcaa tcttgccccc aacaacagtg aattcggtga ccatgcaagc gtcgcaattt
     3601 tcagcccagg ttctggactg gtacgataaa tacgggcgaa aaacgctgcc ctggcaaatt
     3661 gacaagacgc cctacaaagt atggctctca gaagtgatgt tgcaacaaac tcaggttgcg
     3721 accgttatcc cctattttga acgctttatg gcgcgcttcc cgacggtgac cgatctcgcc
     3781 aatgcgccgc tcgacgaagt tctccacttg tggaccgggc ttggctatta cgcccgcgcg
     3841 cgcaatctgc ataaagcggc acaacaagtg gcgaccttac acggcggtaa attcccggaa
     3901 acctttgaag aagtcgcggc gttaccgggc gtcgggcgtt ccaccgcagg cgcgattctc
     3961 tcgctttctc tgggtaagca ctttccgatt ctcgacggta acgtcaaacg cgtgctggcg
     4021 cgctgctatg ctgtaagcgg ctggcctggg aaaaaagagg tcgagaataa attatggagt
     4081 ttgagcgagc aggtgacgcc cgcggtcggc gtggaacggt ttaatcaggc gatgatggat
     4141 ttgggcgcga tgatttgtac gcgctcgaag ccgaaatgtt cgctctgtcc gctacaaaac
     4201 ggatgtattg ccgccgccaa taatagctgg tcgctttatc cgggcaaaaa aacgaaacag
     4261 acgctgccgg agcgcaccgg ctactttttg ctgttacagc acgaagatga agtattgctg
     4321 gcgcagcgtc cgccgagcgg attgtggggc ggtttatact gtttcccgca gtttgccgac
     4381 gaagaaagtt tgcggcagtg gctggcgcaa cggcagattg ctgccgataa cctgacgcaa
     4441 ctgaccgcgt ttcggcatac cttcagccat ttccacttag atattgtgcc tatgtggctt
     4501 cccgtgtcgt cattcaccgg ctgcatggat gaaggcaatg cgctctggta taacttagcg
     4561 caaccgccgt cagttggcct ggcggctccc gtggagcgtt tgttacagca gttacgcact
     4621 ggcgcgccgg tttagcgcgt gagtcgataa agaggatgat ttatgagcag aacgattttt
     4681 tgtactttcc tgcaacgtga agcagaaggt caggattttc agctgtaccc cggcgagctg
     4741 ggaaaacgca tctataacga gatctccaaa gaagcctggg cgcagtggca gcacaagcaa
     4801 accatgctga ttaatgaaaa gaaactcaac atgatgaatg ccgagcaccg caagctgctt
     4861 gagcaggaga tggtcaactt cctgttcgag ggtaaagagg tgcatatcga gggctatacg
     4921 ccggaagata aaaaataaaa acagtgccgg agcacgcctc cggcaacttg cataaaaaca
     4981 aacacaacac gcacccggaa tgatgaaaaa atatctcgcg ctggctttga ttgcgccgtt
     5041 gctcatctcc tgttcgacga ccaaaaaagg cgatacctat aacgaagcct gggtcaaaga
     5101 taccaacggt tttgatattc tgatggggca atttgcccac aatattgaga acatctgggg
     5161 attcaaagag gtggtgatcg ccggtcctaa ggactacgtg aaatacaccg atcaatatca
     5221 gacccgcagc cacatcaact tcgatgacgg tacgattact atcgaaacta tcgccgggac
     5281 agaacctgcc gcgcatttgc gccgggcaat tatcaaaacg ttgctgatgg gtgacgatcc
     5341 gagttcggtc gatctctatt ccgacgttga tgatattacg atttcgaaag aacctttcct
     5401 ttacggtcag gtggtggaca acaccgggca gccgattcgc tgggaaggtc gcgcaagcaa
     5461 cttcgcggat tatctgctga aaaaccgtct gcaaagccgc agtaacgggc tgcgtatcat
     5521 ctacagcgtc accattaaca tggtaccgaa ccaccttgat aaacgtgcgc acaaatatct
     5581 cggcatggtc cgccaggcgt cacggaaata tggcgttgat gagtcgctga ttctggcaat
     5641 tatgcagacc gaatcttcct ttaacccgta tgcggtcagc cgttccgatg cgctgggatt
     5701 aatgcaggtg gtacaacata ctgccgggaa agatgtgttc cgctcgcagg ggaaatccgg
     5761 cacgccgagc cgcagtttct tgtttgatcc tgccagcaat attgataccg gcaccgcgta
     5821 tctggcgatg ctgaacaatg tttatctcga cggaattgat aacccaacat cgcggcgtta
     5881 tgccgtcatc accgcctata acggcggcgc aggcagcgtg ctgcgagtct tttcgaatga
     5941 taagattcag gctgccaata ttattaacac catgacgccg ggcgatgttt atcagacgct
     6001 gacgacccgc catccctctg cggaatctcg ccgttatctt tataaagtga ataccgcgca
     6061 aaaatcctac cgccgccgat aattccatta accgcccctg acgatgctca ggggcaaaaa
     6121 tgttatccac atcacaattt cgttttgcaa attgggaatg tttgcaatta tttgccacag
     6181 gtaacaaaaa accagtccgc gaagttgata gaatcccatc atctcgcacg gtcaaatgtg
     6241 ctttttcaaa cactcatccg catcacaatg tgaggaaatt aacatgaatc ttaagctgca
     6301 gctgaaaatc ctctcttttc tgcagttctg tctgtgggga agttggctga cgaccctcgg
     6361 ctcctatatg tttgttaccc tgaagtttga cggtgcttct attggcgcag tttatagctc
     6421 actgggtatc gccgcggtat ttatgcctgc gctgctgggg attgtggccg acaaatggtt
     6481 aagtgcgaaa tgggtatatg ccatttgcca caccattggc gctatcacgc tgttcatggc
     6541 ggcacaggtc acgacgccgg aagcgatgtt ccttgtgata ttgattaact cgtttgctta
     6601 tatgccaacg cttgggttaa tcaacaccat ctcttactat cgcctgcaaa atgccgggat
     6661 ggatatcgtt actgacttcc cgccaatccg tatctggggc accatcggct ttatcatggc
     6721 aatgtgggtg gtgagcctgt ctggcttcga attaagccac atgcagctgt atattggcgc
     6781 agcactttcc gccattctgg ttctgtttac cctgactctg ccgcatattc cggttgctaa
     6841 acagcaagcg aatcagagct ggacaaccct gctgggcctc gatgcattcg cgctgtttaa
     6901 aaacaagcgt atggcaatct tcttcatctt ctcaatgctg ctgggcgcgg aactgcagat
     6961 taccaacatg ttcggtaata ccttcctgca cagcttcgac aaagatccga tgtttgccag
     7021 cagcttcatc gtgcagcatg cgtcaatcat catgtcgatt tcgcagatct ctgaaaccct
     7081 gttcattctg accatcccgt tcttcttaag ccgctacggt attaagaacg taatgatgat
     7141 cagtattgtg gcgtggatcc tgcgttttgc gctgtttgct tacggcgacc cgactccgtt
     7201 cggtactgta ctgctggtac tgtcgatgat cgtttacggt tgcgcattcg acttcttcaa
     7261 catctctggt tcggtgtttg tcgaaaaaga agttagcccg gcaattcgcg ccagtgcaca
     7321 agggatgttc ctgatgatga ctaacggctt cggctgtatc ctcggcggca tcgtgagcgg
     7381 taaagttgtt gagatgtaca cccaaaacgg cattaccgac tggcagaccg tatggttgat
     7441 tttcgctggt tattccgtgg ttctggcctt cgcgttcatg gcgatgttca aatataaaca
     7501 cgttcgtgtc ccgacaggca cacagacggt tagccactaa ttacgcaaag aaaaacgggt
     7561 cgccagaagg tgacccgttt ttttattctt acttcaacac ataaccgtac aatcgtttca
     7621 tgccatccgc atcggtttcg ctatagacac cttgcaactc cggcgaaaat cccggcaaca
     7681 aattcacgcc ttcttccagc gcaaggaaat aacgttgaac cgccccaccc cagacttccc
     7741 cgggaaccac gcaaagcacg ccaggtggat aaggcaacgc cccttctgcc gcaattcgcc
     7801 cttcggcatc acgaatccgc accaactcca cttcaccgcg aat
//
LOCUS       DAJVRF010000168         7797 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJVRF010000168 DAJVRF010000000
VERSION     DAJVRF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.168"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="OQN13_003526"
     CDS             complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="OQN13_003526"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HCS1616794.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="OQN13_003527"
     CDS             complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="OQN13_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCS1616795.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            2028..2546
                     /locus_tag="OQN13_003528"
     CDS             2028..2546
                     /locus_tag="OQN13_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCS1616796.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(2591..4636)
                     /gene="dcp"
                     /locus_tag="OQN13_003529"
     CDS             complement(2591..4636)
                     /gene="dcp"
                     /locus_tag="OQN13_003529"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCS1616797.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            4773..5519
                     /gene="ydfG"
                     /locus_tag="OQN13_003530"
     CDS             4773..5519
                     /gene="ydfG"
                     /locus_tag="OQN13_003530"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCS1616798.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5608..6294
                     /gene="ydfH"
                     /locus_tag="OQN13_003531"
     CDS             5608..6294
                     /gene="ydfH"
                     /locus_tag="OQN13_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCS1616799.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            6471..6674
                     /gene="ydfZ"
                     /locus_tag="OQN13_003532"
     CDS             6471..6674
                     /gene="ydfZ"
                     /locus_tag="OQN13_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCS1616800.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(6710..>7797)
                     /locus_tag="OQN13_003533"
     CDS             complement(6710..>7797)
                     /locus_tag="OQN13_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS1616801.1"
                     /translation="EKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATGVIVEALARR
                     KAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFPSTMVDRIVP
                     AVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYE
                     EMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAPTLKVQGVDL
                     QDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGVAG
                     WMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPDNSL
                     FTAKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         2051 a         1956 c         1786 g         2004 t
ORIGIN      
        1 cggagataat ccctcaccta accggcccct tgttacagtt gtgtacaagg ggcctgattt
       61 ttatgacggc gaaaaaaaac cgccagtaaa ccggcggtga atgcttgcat ggatagattt
      121 gtgttttgct tttacgctaa caggcatttt cctgcactga taacggatcg ttgacacagt
      181 agcatcagtt ttctcaatga atgttaaacg gagcttaaac tcggttaatc acattttgtt
      241 cgtcaataaa catgcagcga tttcttccgg tttgcttacc ctcatacatt gcccggtctg
      301 ctcttccaat gaccacatcc agaggctctt caggaaatgc gcgactcaca cctgctgtca
      361 cggtaatgtt gatatgccct tcagaatgtg tgatggcatg gttatcgact aactggcaaa
      421 ttctgacacc tgcacgacat gcttcttcat cattagccgc tttaacaata atgataaatt
      481 cttcgccccc gtagcgataa accgtttcgt aatcacgcgt ccaactggct aagtaagttg
      541 ccagggtgcg taatactaca tcgccgatta aatgcccgta ggtatcatta accaatttaa
      601 atcggtcaat atccaacaac attaaataaa gattcagagg atcagtgttg cgtaactgat
      661 gatcaaagga ttcatcaaga acccgacgcc ccggcaatcc cgtcaaaaca tccatattgc
      721 tacggatcgt cagcaaataa attttgtaat cggttaatgc cgcagtaaaa gaaagcaacc
      781 cctcctgaaa ggcgtcgaaa tgcgcgtcct gccagtgatt ttcaacaata gccagcatta
      841 attcccgacc acagttatgc atatgttgat gggcagaatc cattagccga acgtaaggta
      901 attcatcgtt atcgagtggc cccagatgat caatccaccg accaaactgg cacagtccat
      961 aagaatggtt atccgttatt tctggcttac tggcatctct cgcgaccacg ctgtgaaaca
     1021 tactcaccag ccactggtag tgggcatcga tagccttatt gagatttaac aagatggcat
     1081 caatttccgt tgtcttcttg atcattgcca ctcctttttc acagttcctt gtgcgcgcta
     1141 ttctaacgag agaaaagcaa aattacgtca atattttcat agaaatccga agttatgagt
     1201 catctctgag ataacattgt gatttaaaac aaaatcagcg gataaaaaag tgtttaattc
     1261 tgtaaatcac ctctgcatta tcgtaaataa aaggatgaca aatagcataa cccaataccc
     1321 taatggccca gtagttcagg ccatcaggct aatttatttt tatttctgca aatgagtgac
     1381 ccgaacgacg gccggcgcgc ttttcttatc cagactgccg ctaatgttga tcatctggtc
     1441 cggctgaact tctcgtccat caaagacggc cgcaggaata acaacattaa tttcaccgct
     1501 cttatcgcga aaaacgtaac ggtcctctcc tttgtgagaa atcaaattac cgcgtagtga
     1561 aaccgaagcg ccatcgtgca tgttttttgc gaaatcaacg gtcatttttt ttgcatcatc
     1621 ggttccgcga tagccatctt ctattgcatg aggcggcggt ggcgctgcat cctgttttaa
     1681 accgccctgg tcatctgcca acgcataagg catgacaaga aaacttgcta atacaatgga
     1741 ctgaaatttc atactaactc cttaattgcg tttggtttga cttattaagt ctggttgcta
     1801 tttttataat tgccaaataa gaatactgcc aattgttata aggcatttaa aatcagccca
     1861 ctagctgtca aatattcaga gaatttgact cactaaagtt aagaagattg aagagtctta
     1921 aacatgtttt cagaataatc ggatttatat gtttgaaaat tattatattg gacgagcata
     1981 cagaaaaagc aaatcacctt tacatataat agcgtggaca aaaaacagtg aacattaata
     2041 aagataaaat tgtacaactc gcagataccg acactattga aaacctgaca tccgcgttga
     2101 gtcaaagact tatcgcggat caattacgct taactaccgc cgaatcatgc accggcggta
     2161 agttggctag cgccctgtgt gcagctgaag atacacccaa attttacggt gcaggctttg
     2221 ttactttcac cgatcaggca aagatgaaaa tcctcagcgt aagccagcag tctcttgaac
     2281 gatattctgc ggtgagtgag aaagtggcag cagaaatggc aacaggtgcc atagagcgtg
     2341 cggatgctga tgtcagtatt gccattaccg gctacggcgg accggagggc ggtgaagatg
     2401 gtacgccagc gggtaccgtc tggttcgcgt ggcatattaa aggccagaac tacactgcgg
     2461 ttatgcattt tgctggcgac tgcgaaacgg tattagcttt agcggtgagg tttgccctcg
     2521 cccagctgct gcaattactg ctataaccag gctggcctgg cgatatcccc aggccagcca
     2581 ttggtggtgt ttatatgttc aagccacgat gttgcagcat cggcataatc tgaggagcct
     2641 taccgcgcca ttgtcgatgc aggcgttcca gatcttcgct gttacctctg gaaaggatcg
     2701 cctcgcgaaa acgctgccca ttttcacgcg ttaatccgcc ctgctcaaca aaccactggt
     2761 aaccatcatc ggccaacatt tgcgtccaca gataagcgta ataacctgca gcatatccgc
     2821 caccaaaaat atgggcgaaa taactgctgc gatagcgtgg cggtatagca ggaagatcca
     2881 tattttccgc caccagcgcc cgcaattcaa aatcatcgac atcctgcatt gcttcgtttt
     2941 cttccaggca atgccagcgc atatcgagaa gtgcggcgct aagcagttcg ctcatctcat
     3001 accctttgtt gaacaggctg gcattacgca ttttctgttg cagttcgtca ggcattgctg
     3061 ccccgctctg ataatgccgg gcgtagcgag cgaatacctg cggatgcgtt gcccagtgtt
     3121 cgttgatttg cgacggaaat tcgacaaaat cacgcggcgt gttggtgccg gaaagcgtgg
     3181 cataacgctg gcgggcaaaa aggccgtgca gcgtatgacc aaattcatgg aataaggtta
     3241 ttacatcatc ccagagtaac aacgcaggct caccggcagc gggtttctga taattgcaga
     3301 cgttataaat taccggatgc gtttcattaa gcgttgattg ctcaacaaaa ttgcccatcc
     3361 atgcaccgcc gctttttgaa tcacgggcga agaaatcacc gtaaaataac gccagcccca
     3421 cgccattatg atcaaaaatt tcccacaccc gaacgtcagg atggtagaca ggaatatcaa
     3481 aacgttcgac aaacttaata ccgaagagct gattcgcggt ccagaataca ccttcattca
     3541 acaccgtgtt taattcaaaa tatggcttga gctgcgcttc atcaagatca aatttctccc
     3601 gccgtacctg ttccgcataa aatgcccagt cccacggctg cgcgctaaac ccgccctgct
     3661 gcttatcgat aaccgcctgt atggaggcta attcatcgct cgcacgttga cgcgccgctg
     3721 gaacaatttc ccgcataaag ttgagtgctg cttctggcgt ttttgccatc tgatcggcga
     3781 ttttccatgc ggcataatga ggaaaaccaa gcagtttcgc ctgctgtgcg cgaatctcta
     3841 ccagacgttg aatgatagcg cgggtatcat tgccatcatt tttttccgct cgcgtccagc
     3901 ccgcaataaa cagtttttca cgcgtcgcac gatcgcgcat ttcggcaagc gccggttgtt
     3961 gggtggtatt cagcagcgga atcagccatt tgttatccag acctttctcg cgagccgcct
     4021 ctgccgccag cgcaatctct tgctcactca ttcctgccag ctgcgcgata tcgttcacaa
     4081 ccagaccgcc ggatttattt gctgccagta atcgctggtt aaactggctg gtcagggtcg
     4141 cagcttctgt attcagtact tttaattttg ctttatcagc ttgcgcaagt ttggctccgg
     4201 caaggacaaa acgttgatga atcacctcca ccaggcggat ggattcacta tcaagcccca
     4261 gggattcacg gcgctgccag acagcatcta cccgcgcgaa taattcaccg ttcagataga
     4321 tatcattagc cagttccgcc agttcagcgg aaaactgttc atcaagacgc tgtaattcat
     4381 cattggtatg cgccgcagtc atcgcaaaaa agacgctggt aacgcgggta agtaattctc
     4441 cgctttgttc cagtgccaga atagtattgt tgaaatcagg cgtttgcggg ttaagcgcga
     4501 tggcagcaat ttctgcccgc ttttgctgca ttccctcatc gaatgccggg cgatagtgat
     4561 gattggcaat ttgatcaaaa tggggagcca gatacggtag tgtgctttgc acaaggaaag
     4621 gattcattgt tgtcattttc ttctcctgaa cgcgagatgt tccatagcgt aggcttactg
     4681 ataacggagt gcaatcttgc aaaccagtat tacccgctct taagcatcac gtgctatgtt
     4741 attgacacac aaaagcgttg aggaacagtg agatgatcgt tttagtaact ggagcaacgg
     4801 caggttttgg tgaatgcatt actcgtcgtt ttattcaaca agggcataaa gttatcgcca
     4861 ctggccgtcg ccaggagcgg ttgcaggagt taaaagacga actgggagat aatctgtata
     4921 tcgcccaact ggacgttcgc aaccgcgccg ctattgaaga gatgctggca tcgcttcctg
     4981 ccgagtggtg caatattgat atcctggtaa ataatgccgg cctggcgttg ggcatggagc
     5041 ctgcgcataa agccagcgtt gaagactggg aaacgatgat tgataccaac aacaaaggcc
     5101 tggtatatat gacgcgcgcc gtcttaccgg gtatggttga acgtaatcat ggtcatatta
     5161 ttaacattgg ctcaacggca ggtagctggc cgtatgccgg tggtaacgtt tacggtgcga
     5221 cgaaagcgtt tgttcgtcag tttagcctga atctgcgtac ggacctgcat ggtacggcgg
     5281 tgcgcgtcac cgacatcgaa ccgggtctgg tgggtggcac cgagttttcc aatgtccgct
     5341 ttaaaggcga tgacggtaaa gcggaaaaaa cctatcaaaa taccgttgca ttgacgccag
     5401 aagatgtcag cgaagccgtc tggtgggtgt caacgctgcc tgctcacgtc aatatcaata
     5461 ccctggaaat gatgccggtt acccaaagct atgccggact gaatgtccac cgtcagtaat
     5521 ttttataccc ggcgtaactg ccgggttatt gcttgtcaca aaaaagtggt agactcatgc
     5581 agttaactca ctcacaagca agaacgaatg accgtcgaaa cgcaacttaa tcccacacag
     5641 cctgtcaatc agcaaattta tcgtattctt cgtcgcgaca ttgtccattg cctgattgca
     5701 ccaggcacac cgttgtcgga aaaagaagtt tctgttcgtt tcaatgtgtc acgccagccg
     5761 gttcgtgaag cctttattaa actggcggaa aacggcctga ttcaaattcg tccgcagcgt
     5821 ggcagctacg tcaacaaaat ttccatggcc caggtgcgca atggcagttt tatccgtcag
     5881 gccattgagt gcgcggtggc gcgtcgggcg gcgagcatga ttaccgaaag ccagtgctac
     5941 caactggaac aaaatcttca ccagcaacgc attgccattg agcgcaagca actggatgat
     6001 ttttttgaac ttgatgacaa cttccatcaa ctcctgacgc agattgccga ctgtcaactg
     6061 gcgtgggata ccattgagaa cctgaaagcg accgttgatc gcgtgcgcta tatgagtttc
     6121 gaccacgttt ctccaccaga aatgctgtta cgccagcatc ttgatatttt ctctgccctg
     6181 caaaaacgtg atggcgatgc ggtagaacgt gcaatgacgc aacatttgca ggaaatcagc
     6241 gaatccgtgc gccagatccg ccaggaaaat agcgactggt ttagcgaaga gtaattcatt
     6301 tcctctcatc ccatccgggg tgagagtctt ttcccccgcc ttatggctca tgcatgtatc
     6361 aaaaaagatg tgagcttgat caaaaacaaa aaatatttca ctcgacagga gtatttatat
     6421 tgcgcccgtt acgtgggctt cgactgtaaa tcagaaagga gaaaatacct atgacgacct
     6481 acgatcgtaa ccgtaacgca atcaccactg gcagccgtgt tatggttagc ggcaccggtc
     6541 acactggcaa gatcctgtcg attgatactg aaggtctgac cgctgagcaa atccgccgcg
     6601 gaaaaaccgt agttgttgaa ggttgtgaag agaaactggc acctctggac ctgattcgtc
     6661 tcggcatgaa ctaagcgtgt gaatgccgcc gatggcggca ttgctttttt tacttcacgg
     6721 aatatttcgc cacggtcgct ttcgcgccat gcgctaataa agacaagtac gcttccgtca
     6781 cttttgcagt aaacaaacta ttgtctggca aatcatcacc aaagatcgcc ttaatcgcca
     6841 gcaatgactg gacgcgcgct ttcccttcag cactactttg tacagccttc tgaataacag
     6901 gtaacagtgg gtcactgatt tcaatcggat ttccctgttc atcaacacca ccgacataac
     6961 gcatccaacc cgcgacgccc agtgccagca gatcgaactt gctgtcatgc gccagatgcc
     7021 agcgaacaga atccaacatc cgctgtggca atttctggct gccatccatc gcaatctgcc
     7081 aggttcgatg acgtaacgcc gggttgctat agcgttcaat taatcggtta gcgtaatctt
     7141 gcaaatcaac gccctgcact ttcagcgtcg gcgcttgttc ctgcaacatc aagccatacg
     7201 ccgcataacg ataatgttca tcttccatgc agtcattaat gtgctgatat ccagcaagat
     7261 aacccaaata cgccaggaat gaatgactgc cgttgagcat gcgcaacttc atctcttcat
     7321 aaggcagcac atcactaacc agttcggctc ccgctttttc ccattctgga cgtccggcaa
     7381 caaagttatc ttctattacc cactggcgga aaggttcaca ggcaacgccc gcaggatcgc
     7441 ccacaccggt aagttgttcg attttcgcca gcgtatcctc tgtcactgcg ggcacaatac
     7501 ggtccaccat tgttgatggg aaagtcacgt tatcttcgat ccattgtgcc agttttacat
     7561 caacagcttt cgcgtatgaa gtgacaacgt cacgcataac atgaccgttt tctggcatgt
     7621 tgtcacatga catgacggta aatgcgggaa gtcctgccgc tttacggcgg gccagcgcct
     7681 caacaatcac ccctgttgct gttttcggct ggtggggatt ttgcacgtcg gcagcgacca
     7741 tcgggtgatc gagcattaac tgtccggtcg ccggagagtg gaaataccct ttttcgg
//
LOCUS       DAJVRF010000169         7716 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJVRF010000169 DAJVRF010000000
VERSION     DAJVRF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7716
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.169"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..644
                     /locus_tag="OQN13_003534"
     CDS             <1..644
                     /locus_tag="OQN13_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1616802.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            895..2457
                     /gene="hpaB"
                     /locus_tag="OQN13_003535"
     CDS             895..2457
                     /gene="hpaB"
                     /locus_tag="OQN13_003535"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HCS1616803.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHATLQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2475..2987
                     /locus_tag="OQN13_003536"
     CDS             2475..2987
                     /locus_tag="OQN13_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCS1616804.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3380..5530
                     /gene="btsT"
                     /locus_tag="OQN13_003537"
     CDS             3380..5530
                     /gene="btsT"
                     /locus_tag="OQN13_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCS1616805.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5580..5783
                     /locus_tag="OQN13_003538"
     CDS             5580..5783
                     /locus_tag="OQN13_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCS1616806.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5794..6750
                     /gene="yjiA"
                     /locus_tag="OQN13_003539"
     CDS             5794..6750
                     /gene="yjiA"
                     /locus_tag="OQN13_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCS1616807.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6828..>7716)
                     /locus_tag="OQN13_003540"
     CDS             complement(6828..>7716)
                     /locus_tag="OQN13_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCS1616808.1"
                     /translation="RVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWL
                     TKQDVEAHKESQEVLHVQR"
BASE COUNT         1785 a         2102 c         2020 g         1809 t
ORIGIN      
        1 ctcatgcgtt tattacggag tctgacgccg acggtcatgt gttgacggta cgggaagatc
       61 tgatctggcc cctgctggaa gttctttatc caggcactcg ggaaaccttc ggcctgccgg
      121 ggatttgcct gtcactggca gataaacccg acgaactggc ggcgctggaa cactattggc
      181 aactgataga gcgggaatcg gtggaacaac tgcctggacg ggaacacacc ctgacgctac
      241 tggcacaggc agtgttcacc ctactgctgc gtaatgcaaa actcgacgac catgccgcca
      301 gcgggatgcg cggagaatta aaactgttcc agcgttttaa tatgctgatt gaaagccatt
      361 ttcatcagca ctggacagta ccggattacg ctaacgaact gcatatcacc gaatcacgcc
      421 tcacggacat ctgccgccgc tttgccaacc gtccgccaaa acggttgatt tttgacaggc
      481 agctgcgaga agccaagcgg ctgctgctgt tttctgataa cgccgtgaac aatattgcct
      541 ggcaactcgg ttttaaggat ccagcttatt ttgcgcgctt ttttaatcgc ttagtcggtt
      601 gctcgcccag tgcttatcgt gccaaaaaag tacctgtgac gtgaaaatcc cttagcctta
      661 acgggaaacc aggcaccacc tgctattccc ctttctccgc ctggagcaag gggatttagt
      721 aacgtcaaaa atcgctaaaa gcgaccgcga tcacaaaacc gtacccgacc tgaaaagtac
      781 cagccattgt cccaaaagtg tcttcccgta atcgcccctg caagcttcaa ttgaataaca
      841 aaaaaaacac acaaaattaa caatcgtatt ccgattaata ctgtagaggt cgacatgaaa
      901 ccagaagatt tccgcgccag tacccaacgt ccgttcaccg gggaagagta tctgaaaagc
      961 ctgcaggatg gtcgcgagat ctatatctat ggcgagcgag tgaaagacgt cactactcat
     1021 ccggcatttc gtaatgcggc agcgtctgtt gcccaactgt acgacgcgct gcacaaaccg
     1081 gagatgcagg actctctgtg ctggaacacc gacaccggca gcggcggcta tacccataaa
     1141 ttcttccgcg tggcgaaaag tgccgacgac ctgcgccagc aacgtgacgc catcgctgag
     1201 tggtcacgcc tgagctatgg ctggatgggc cgtaccccag actacaaagc tgctttcggt
     1261 tgcgcactgg gcgcgaatcc gggcttttac ggtcagttcg agcagaacgc ccgtaactgg
     1321 tacacccgta ttcaggaaac tggcctctac tttaaccacg cgattgttaa cccaccgatc
     1381 gatcgtcatt tgccgaccga taaagtgaaa gacgtttaca tcaagctgga aaaagagact
     1441 gacgccggga ttatcgttag cggtgcgaaa gtggttgcca ccaactcggc gctgactcac
     1501 tacaacatga ttggcttcgg ctcggcacaa gtaatgggcg aaaacccgga cttcgcactg
     1561 atgttcgttg cgccaatgga tgccaatggc gtcaaattaa tctcccgcgc ctcttatgag
     1621 atggtcgcgg gtgctaccgg ctcgccgtac gactacccgc tctccagccg cttcgatgag
     1681 aacgatgcga ttctggtgat ggataacgtg ctgatcccat gggaaaacgt actgatctac
     1741 cgcgatttcg atcgctgccg tcgctggaca atggaaggcg gttttgcccg tatgtatccg
     1801 ctgcaagcct gtgtgcgcct ggcagtgaaa ctcgacttca ttacagcact gctgaaaaaa
     1861 tcactcgaat gtaccggcac cctggagttc cgtggtgtac aggccgatct cggtgaagtg
     1921 gtggcgtggc gcaacacctt ctgggcattg agtgactcga tgtgttctga agcgacgccg
     1981 tgggtcaacg gggcttattt accggatcat gccacactgc aaacctatcg cgtactggca
     2041 ccaatggcct acgcgaagat caaaaacatt atcgaacgca acgttaccag tggcctgatc
     2101 tatctccctt ccagtgcccg tgacctgaac aatccgcaga tcgaccagta tctggcgaag
     2161 tatgtgcgcg gttcgaatgg tatggatcac gtccagcgca tcaagatcct caaactgatg
     2221 tgggacgcca ttggcagcga gtttggtggt cgccacgaac tgtatgaaat caactactcc
     2281 ggtagccagg atgagattcg cctacagtgt ctgcgccagg cacaaagctc cggcaatatg
     2341 gacaagatga tggcgatggt tgatcgctgc ctgtcggaat acgaccagaa cggctggact
     2401 gtgccgcact tgcacaacaa cgacgatatc aacatgctgg ataagctgct gaaataacgc
     2461 agcaggaggt taagatgcaa ttagatgaac agcgcctgcg ctttcgtgac gcgatggcca
     2521 gcctgtcggc agcggtaaat attatcacca ccgagggcga cgccggacaa tgcgggatta
     2581 cggcaacggc cgtctgctcg gtcacggata caccaccatc gctgatggtg tgcattaacg
     2641 ccaacagtgc gatgaacccg gtttttcagg gcaacggtaa gttgtgcgtc aacgtcctca
     2701 accatgagca ggaactgatg gcacgccact tcgcgggcat gacaggcatg gcgatggaag
     2761 agcgttttag cctctcatgc tggcaaaaag gtccgctggc gcagccggtg ctgaaaggtt
     2821 cgctggccag tcttgaaggt gagatccgcg atgtgcaggc aattggcaca catctggtgt
     2881 atctggtgga gattaaaaac atcatcctca gtgcagaagg tcacggactt atctacttta
     2941 aacgccgttt ccatccggtg atgctggaaa tggaagctgc gatttaagct ccagcccctt
     3001 cacgcattcg tcgaaggggc atttttattg tgatctcaat cacatatcca cccgatgaat
     3061 aattcttgcc caaccgctca ccgccattcc tgaccgctta cgtccctaaa ataaccactc
     3121 agttatttac cttactttac gcgcgcgtaa ctctggcaac atcactgcag gatagcggtc
     3181 aatttacctc ctcaaacgca acgcaaacct agaacggctt cggcctatga ttaacaaatt
     3241 actctatgaa ttttggtttg caggaaggca gcaagtgagt gaatccccgg gagcttacaa
     3301 cagtaagtga caggggtgaa cgaacgcaac tgccgcacct gtaagccaaa agacgacgag
     3361 taaaatgcca ggttttacta tggatacgaa aaaactattc aagcacatac cctgggtgat
     3421 tctcggaatc atcggtgcat tctgcctcgc ggtagttgca ttacgtcggg gggagcacgt
     3481 cagcgccctg tggatcgtgg tcgcctctgt gtcggtgtat ctggtggcgt atcgctacta
     3541 cagtctgtac atcgcccaga aggtgatgaa actcgacccc acgcgcgcga cgcctgcggt
     3601 tattaacaac gacggtctga actacgttcc gaccaaccgt tacgtgttgt ttggtcacca
     3661 cttcgccgct atcgccggtg ctggtccgct ggtcggtccg gttctcgccg cacagatggg
     3721 ccacctgcct ggcacgctgt ggctgctggc gggggtagta ctggccggtg cggttcagga
     3781 ctttatggtg ctgtttatct cctctcgccg taacggcgca tctctcggtg agatgataaa
     3841 agaagagatg ggaccagtac cggggactat cgcgctgttt ggctgtttct taatcatgat
     3901 catcatcctt gccgtcctgg cgctgattgt ggttaaagcc ctggccgaaa gtccgtgggg
     3961 tgtcttcacc gtttgctcaa ccgtaccgat tgcgctgttt atgggtatct acatgcgctt
     4021 tatccgtccg ggacgtgtgg gtgaagtctc tgtcattggt atcgtgctgc tggttgcctc
     4081 tatctacttc ggtggcgtga ttgctcacga tccgtactgg ggtccggcac tgacctttaa
     4141 agacaccacc attaccttcg cgctgattgg ctatgcgttt gtttccgcat tgctgccggt
     4201 gtggctgatc ctcgcaccgc gcgactatct ggcaaccttc ctgaaaatcg gcgttatcgt
     4261 cggcctggcg ctgggtatcg tggtgctgaa cccggaactg aaaatgcctg cgatgaccca
     4321 gtacattgac ggtactggcc cgctgtggaa aggtgctctg ttcccgttct tgttcattac
     4381 catcgcctgt ggtgcggtat ctggcttcca cgcactgatc tcttccggta cgacgccgaa
     4441 actgctggct aatgaaaccg acgcgcgttt cattggctac ggcgcaatgc tgatggagtc
     4501 cttcgtggcg attatggcgc tggttgctgc gtccatcatc gaaccgggtc tttacttcgc
     4561 gatgaacacc ccgcctgctg gccttggcat caccatgcct aacctgcatg aaatgggtgg
     4621 cgagaacgcg ccgatcatca tggcgcagct gaaagacgtt accgcacacg cggcagcgac
     4681 cgtcagctct tggggcttcg tgatttcgcc agagcagatc ctgcaaaccg cgaaagacat
     4741 tggtgagcct tctgtcctga accgtgcagg tggtgcgcca acgctggcgg taggtatcgc
     4801 tcacgtgttc cacaaagtgc tgccgatggc tgacatgggc ttctggtatc acttcggtat
     4861 tctgttcgaa gccctgttca tcctgaccgc gctggatgcg ggtacccgtt ctggccgttt
     4921 tatgctgcaa gacctgctgg gtaacttcat cccgttcctg aaaaaaaccg actctctggt
     4981 tgccggtatc atcggtactg cgggctgtgt gggtctgtgg ggctacctgc tgtatcaggg
     5041 cgtggtcgat ccgctgggcg gcgttaagag cctgtggccg ctgttcggta tctccaacca
     5101 gatgctggca gccgtagcgc tggtactggg caccgttgtg ctgattaaga tgaagcgcac
     5161 ccaatacatc tgggtaactg ttgttccggc tgtatggctg cttatctgca ccacctgggc
     5221 gctgggtctg aaactgttca gcaccaaccc gcagatggaa ggcttcttct acatggcaag
     5281 ccagtacaaa gagaagattg ctaacggtac tgacctgacg gcgcagcaga ttgccaacat
     5341 gaaccacatc gttgtgaaca actacaccaa cgcaggcctg agtattctgt tcctgattgt
     5401 ggtgtacagc atcatcttct acggtttcaa aacctggctt gcggtgcgta acagcgacaa
     5461 acgtactgac aaagaaactc cgtacgttcc aatcccggaa ggcggcgtga agatctcttc
     5521 gcaccactaa ccgtgtttag ccccgcttcg gcggggcttt gttcttatca gagtgaacta
     5581 tgtttggtaa cttaggacag gcaaaaaaat atctcggcca ggcggcgaag atgttgattg
     5641 gcattccaga ctacgacaac tacgtcgagc atatgaagac caaccatccc gacaagccgt
     5701 acatgagcta tgaagaattc ttccgcgaac gccagaacgc gcgctacggc ggcgatggta
     5761 aaggcggtat gcgctgttgt taatggagag agcatgaacc cgattgcagt taccctactc
     5821 accggttttt taggcgcggg caaaaccacc ctgctgcgcc atattcttaa cgaacaacac
     5881 ggctacgaga ttgccgtgat tgaaaacgaa ttcggcgaag tctccgttga tgatcaactg
     5941 attggcgacc gcgccacgca gatcaaaacg ctgaccaacg gctgcatctg ctgttcgcgc
     6001 tccaacgagc tggaagacgc actactcgac ctgctggata atctcgacaa gggcaatatt
     6061 cagttcgacc gtctggtcat tgaatgcacc ggcatggccg atcccggccc gattattcag
     6121 accttcttct cccatgagat tttatgccag cgctacctgc tggacggggt gattgcgctg
     6181 gtggatgcgg tacatgccga tgagcagatg aaccagttca ccatcgccca gtcccaggtt
     6241 ggctacgccg accgtattct gctgacgaaa accgacgtcg caggcgaagc agagaaactg
     6301 cgtgaacgcc tggcgcgcat caacgcccgc gcaccggtct acaccgttac ccacggcgat
     6361 atcgacctgg gccttctgtt caacaccaac ggttttatgc tggaagaaaa cgtcgtcagc
     6421 accaaaccgc gtttccactt tatcgcggac aaacaaaacg atatttcgtc gattgtggtg
     6481 gaactggatt acccggtaga tatcagcgaa gtttcccggg tgatggaaaa cctgctgctg
     6541 gaatcggcgg ataaactgct gcgttacaaa gggatgctgt ggattgacgg cgaacctaac
     6601 cgcctgctgt tccagggcgt ccagcgcctc tacagcgccg actgggaccg cccgtggggc
     6661 gatgaaaaac cgcatagcac gatggtgttt atcgggattc agttgccgga ggatgagatt
     6721 cgggcggcgt ttgcgggact caaaaagtaa ccagccctcc aacatcacaa tacctgagac
     6781 tttatcaccc tcatattcct ccagaatatg agggcattcg ggcttaatta tctctgaacg
     6841 tgcaacactt cctgcgactc cttatgcgcc tccacatcct gcttcgttaa ccagagcgac
     6901 ttacgaccaa aattttcgta cattttcagc tgatcttcat agtgctgatc aactgttcca
     6961 tcgggagcaa taaacccact ctgaccgggt gcgaccacat cccaggcaag caccggacga
     7021 tcgcttgtcg ttggtgagaa aacaatcata tcgttttctg ttccacggtt ttgatactcc
     7081 gcctgatgac gcgtttcttc cgctgcggcc tgcggtacac caaagaaatt atttgcacgg
     7141 aacgttaagg ccattgcagg tgttttccag ttactcacat tattgccata gcgtttggaa
     7201 agagtctccc aggtatcttc cagcgcagcc aacacaacct cctgctgtgg tttcccagca
     7261 aacagatcaa ccgcctgtgg gattggtgat ttgtctccct gcaccgcctc atacaaaatt
     7321 tttgctccaa cacttatatt cagcgaacca gttgggccgt cctgggttgt ttcgtagcca
     7381 ctggcgctgt accacttatc aaatggcata ggtacggcag ccactacggt acgcttcaac
     7441 atactggtca gccaaacgtt caggatggca gagcctggct gctgccaggt tttaccatca
     7501 tcattaagca aattgatgcc atcccaacgt gttaatgtat ccaccaactg acggcgcgga
     7561 tcgctctgtg tcaaaccaga tgtcgctgct tgcagagtag gtaaaaaaag cctcaggtta
     7621 agatcctgac gactggtttg gcgaataaca tcccatgcct gatcagcagt taagcgtggc
     7681 ttttgctcaa gcagtcggtc gatctccgta acgcga
//
LOCUS       DAJVRF010000170         7609 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJVRF010000170 DAJVRF010000000
VERSION     DAJVRF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7609
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.170"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            163..317
                     /locus_tag="OQN13_003541"
                     /pseudo
     CDS             163..317
                     /locus_tag="OQN13_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            363..779
                     /gene="hicB"
                     /locus_tag="OQN13_003542"
     CDS             363..779
                     /gene="hicB"
                     /locus_tag="OQN13_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HCS1616809.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            858..2264
                     /locus_tag="OQN13_003543"
     CDS             858..2264
                     /locus_tag="OQN13_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCS1616810.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRE
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            2509..3654
                     /locus_tag="OQN13_003544"
     CDS             2509..3654
                     /locus_tag="OQN13_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1616811.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            3672..4685
                     /locus_tag="OQN13_003545"
     CDS             3672..4685
                     /locus_tag="OQN13_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1616812.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            4686..5586
                     /locus_tag="OQN13_003546"
                     /pseudo
     CDS             4686..5586
                     /locus_tag="OQN13_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            5576..6370
                     /locus_tag="OQN13_003547"
     CDS             5576..6370
                     /locus_tag="OQN13_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1616813.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            6392..>7609
                     /locus_tag="OQN13_003548"
     CDS             6392..>7609
                     /locus_tag="OQN13_003548"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCS1616814.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHAASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGL"
BASE COUNT         1761 a         1826 c         2102 g         1920 t
ORIGIN      
        1 ttcagttttg caaattagcg caaagaaatt ctggaatctt ccttcctgat tttgcattgc
       61 attctgccgt tgcggcgatt tagtgctatt ttcgagcaaa ttacacatag gggtaaacac
      121 taatgtttac tttattgata tactcagcgg cagggaggcg atgtgaaaca aagcgagttc
      181 agacgttggc tcgaatctca gggcgtcgat gtagcgaatg gccgcaacca tttgaaactc
      241 aggtttcatg ggaggcgcag tgtcatgccg cgtcactcct gcgtaaagca atcctgaaac
      301 aactcggttt gagttaatcg ccaattaaaa aggttaatga catgcgagag acagtcgaaa
      361 ttatgcgtta tcccgtcact cttacacccg cgccggaagg cggttatatg gtttcttttg
      421 tggatatccc tgaagcgttg acgcagggcg aaactgtcgc tgaagcgatg gaagcggcaa
      481 aagatgcttt actgaccgca tttgattttt attttgaaga taacgagctt atcccgttac
      541 cttcgccatt aaatagtcat gatcacttta ttgaagtacc tttgagcgtc gcctctaaag
      601 tattgctgtt aaatgctttt ttacagtcag aaatcactca gcaagagtta gccaggcgaa
      661 ttggcaaacc taaacaggag attactcgcc tatttaactt gcaccatgcg acaaaaatcg
      721 acgccgtcca gcttgcggca aaggcgcttg gcaaagagtt atcgctggtg atggtttaat
      781 tacagttaac gaaaagttgt catttttaac aactgatata gactgccgaa tcatctgcac
      841 ataattacga ttcgataatg aaaaaatacc agcagcttgc agaacaatta cgcgagcaga
      901 ttgcgtcggg tatctggcaa cccggcgatc gtttgccttc gttgcgtgac caggtggcgc
      961 tttcaggcat gagctttatg actgtcagcc atgcctatca gttgctcgaa agtcagggat
     1021 atattatcgc acgaccgcag tcgggttatt acgttgcgcc acaggcaata aaaatgccga
     1081 aagcgccagt cattccagtc actcgagatg aagcagtcga tatcaacact tatatttttg
     1141 atatgttgca ggccagtcgc gaaccgtcgg tcgttccgtt tgcctcggcc tttcccgacc
     1201 cgcgactttt ccccctccaa caactaaacc gctcgctggc gcaggtaagc aaaaccgcca
     1261 cagcgatgag cgtgattgaa aacttaccgc caggaaacgc agaactgcgt caggctattg
     1321 ctcgtcgcta tgccttacag ggcatcacca tttctcctga tgaaattgtc attactgccg
     1381 gggcgttaga ggcattaaac ctcagtttgc aagcggtaac tgaaccgggc gattgggtga
     1441 tagtagagaa tccttgtttc tacggtgcgt tgcaggcgct ggagcggcta cggctgaagg
     1501 cgttatcggt ggcgacggat gttaaagaag ggatcgatct tcaggcgctg gaactggcgt
     1561 tgcaggatta tccggtgaaa gcgtgctggc tgatgactaa tagccagaac ccactcggat
     1621 ttaccttaac gccgcaaaaa aaagcacaac tggtggcgtt actcaatcag tacaacgtaa
     1681 cgctgattga agatgacgtt tacagcgaac tttattttgg acgggaaaaa ccgctgcctg
     1741 cgaaagcgtg ggatcgccac gatggcgttt tgcattgctc ttcgttttcg aaatgtctgg
     1801 tgcctggttt tcgtattggt tgggtcgccg ccggaaaaca tgcacgtaaa attcaacgct
     1861 tgcagttgat gagtacgctt tccaccagct caccgatgca acttgcgctg gtggattacc
     1921 tttccacgcg ccgatacgac gcccatcttc gtcgcctgcg tcgccagctt gcggaacgta
     1981 aacaacgtgc ctggcaggca ctgctgcgtt atctgcctgc ggaagttaaa attcatcata
     2041 gtgacagcgg ttattttctc tggctggagc tccccgagcc gttagatgcc ggcgaattaa
     2101 gcctggcggc actgacgcat catatcagta ttgcgccggg taaaatgttt tctaccggtg
     2161 aaaactggtc acgttttttt cgttttaata ccgcgtggca gtggggagaa cgtgaagaac
     2221 aggcggtaaa acaattaggc aaacttattc aagaacggct gtaatagcgt ttaatttaat
     2281 tcctcttaga ttgggtaata tgaatttcga atagcagtca tatttcctaa ccccttgtct
     2341 atactccaga agataacctt acagacggca taatgcgcgg tagctcacaa cctgattaaa
     2401 ttttctcagg gacgaaggtg tgcctgcaag ccgccgtcta tggttaaaca aggagatatt
     2461 tttacggcac ggcggctgaa caattaatta cgacaggagt aagaccttat gagcaagaca
     2521 tttgcccgca gcagcctgtg tgcgctcacc atgacaataa tgaccgctca cgccgccgaa
     2581 ccacctacca atttagataa accggaaggg cgactggata ttatcgcctg gccgggatac
     2641 atcgaacgcg gacaaactga taaacaatac gactgggtaa cgcaatttga aaaagagaca
     2701 ggctgcgcgg tgaatgtgaa aaccgccgcg acttccgatg aaatggtcag tctgatgacc
     2761 aaagggggtt acgatctggt tacggcatcc ggcgatgcct cgctgcgttt gattatgggc
     2821 aaacgcgtgc agccgattaa taccgccttg attcccaact ggaaaacgct cgatccgcgc
     2881 gtggttaaag gcgactggtt taatgttggc ggcaaagttt acggcacacc ttaccaatgg
     2941 gggccgaacc tgctgatgta caacactaaa accttcccga cgccgccgga tagctggcaa
     3001 gtggtttttg ttgagcaaaa tctgccggac ggcaagagca ataaaggccg cgttcaggct
     3061 tatgatggcc ctatctacat tgcggacgct gcgttgttcg ttaaagccac tcagccgcag
     3121 ttgggcatca gcgatccgta tcaactaacc gaagaacagt accaggcggt gctgaaagtg
     3181 ctgcgcgctc aacacagttt gatccatcgc tactggcatg acactaccgt gcaaatgagc
     3241 gatttcaaaa acgagggtgt ggttgcttcc agtgcctggc cctatcaggc caacgccctg
     3301 aaagccgaag gccagcctgt tgctaccgtt ttcccgaagg agggtgttac cggttgggct
     3361 gataccacca tgctgcatag cgaagcgaaa catccggttt gcgcctacaa atggatgaac
     3421 tggtcattaa cgccaaaagt gcagggcgat gtggcggcct ggtttggctc gttaccggta
     3481 gtgccggaag ggtgtaaagc cagtccgtta ttaggcgaaa aaggttgtga aaccaacggt
     3541 tttaactatt tcgacaaaat cgccttctgg aaaacgccta tagcagaagg gggcaagttt
     3601 gttccctaca gtcgctggac gcaggattac attgccatta tgggcggtcg ctaacttcgc
     3661 tggggtgctt tatgacgtac gcagtggagt ttgacaacgt ctcgcggttg tacggtgacg
     3721 tgcgcgcagt agatggcgtc agtattgcga taaaagatgg tgagttcttc tctatgctgg
     3781 ggccgtccgg ctccggcaaa accacctgcc tgcgcctgat tgctggcttc gaacagcttt
     3841 ccggcggggc tatctctatc tttggtaaac ccgccagcaa tctgccaccg tgggagcggg
     3901 acgtgaatac tgtctttcag gactacgcgc tatttccgca tatgtcgatt cttgacaatg
     3961 tcgcctatgg gctgatggtc aaaggcgtga ataaaaagca gcggcacgca atggcgcaag
     4021 aggcgctgga gaaagtggcg ttggggtttg tacagcaacg taaaccgtca caactttctg
     4081 gtggtcagcg ccagcgggtt gctatcgcca gagcattggt gaatgaaccg cgcgtattgc
     4141 tgttggatga accgctcggc gcactggatc tcaaattgcg tgagcagatg cagctggaac
     4201 tgaaaaaact gcaacagtct ctcggtatca cttttatctt cgttacccac gatcagggcg
     4261 aagcgttatc gatgtccgat cgtgtggcgg ttttcaataa tggacgcatt gagcaggtcg
     4321 attccccgcg cgatctctat atgcgcccgc gcacgccgtt tgttgccggg ttcgttggta
     4381 catcgaatgt ttttgatgga ctgatggcag agaaactttg tggcatgacg ggaagcttcg
     4441 ccctgcgacc ggaacatatc cgcctcaaca cccctggtga actgcaggcc aatggcacga
     4501 tccaggcggt gcaatatcag ggcgcggcaa ctcgttttga actgaaattg aacggcggtg
     4561 aaaaactgct tgtgagtcag gccaatatga caggcgaaga actgcctgcc acgctcacgc
     4621 ccggacaaca ggtgatggtt tcctggtcgc gtgatgtgat ggtgccgctg gttgaggaga
     4681 ggtgaatggc gatgaatgta ttgcaatcac cttcacgtcc aggtctgggg ctgtttttac
     4741 tgctgcttgg cccgctaatg tggtttggca ttgtctattt cggctcgctg ctgacactgt
     4801 tatggcaggg attttatact tttgacgatt tcaccatgtc ggtaacgccg gaactgacgc
     4861 tggcgaatat ccgtgcgctg tttaatccgg cgaattacga catcattctc cgcacgctga
     4921 ctatggctgt ggcagtcact atcgccagcg ccattctggc ttttccaatg gcgtggtata
     4981 tggcgcgcta taccagcggg aaaatgaaag cgtttttata ttgcggtaat gttgccgatg
     5041 tgggcgagct acattgttaa agcctatgcc tggacgttat tgctggcaaa agatggcgtg
     5101 gctcagtggt ttttacaaca tcttgggctg gaaccactgc tgactgcgtt ccttacatta
     5161 cctgcggtgg gcggaaatac gctgtcaact tccgggctgg ggcgctttct ggtgtttctc
     5221 tatatctggt tgccgttcat gatcctgccc gttcaggcgg cgcttgagcg tttgccgccg
     5281 tcattgttgc aggcgtcggc tgatctcggc gcacgtccac gacaaacctt tcgctatgtg
     5341 gtgctgccgc tggcaatccc gggtattgcc gctggctcta tctttacctt ctcactcact
     5401 ctgggcgatt ttatcgtccc gcagctggtt ggtcctccag gatattttat cggcaatatg
     5461 gtttattccc agcagggggc gattggcaat atgccgatgg cggcggcatt caccttggtg
     5521 ccgattattc tcatcgcact gtacctggcg ttcgtgaaac gtctgggagc gttcgatgca
     5581 ctctgaacgc gcaccgtttt tcctcaaact ggcggcctgg ggcggcgttg ttttcctaca
     5641 ttttcccatc ctgataatcg ccgcctatgc gtttaacact gaagatgcgg cgtttagttt
     5701 tccaccgcag ggcctgacgc tgcgctggtt tagcgtggca gcacagcgta gtgatattct
     5761 tgatgccgtg acactgtcac ttaaagtggc ggcgctggcg acattaattg cgctggtgtt
     5821 agggacgctg gcagctgccg cgctgtggcg acgagacttt ttcggcaaaa acgccatttc
     5881 gctgttactg ctgctgccca ttgcgctgcc gggcagcgtc actggtctgg cgttattaac
     5941 cgcctttaaa accatcaatc tggagccggg atttttcacc atcgtggtcg gtcatgcgac
     6001 tttttgtgta gttgtggtgt ttaacaatgt catcgcccgt tttcgccgca cctcctggag
     6061 tctggttgag gcgtcaatgg atcttggggc caatggctgg caaaccttcc gctacgtagt
     6121 gttgccgaat ctcagttcgg cgttactggc aggaggaatg ctggcgtttg ccttgtcgtt
     6181 cgatgaaatc atcgttacga cctttacggc aggtcatgaa cgaacgttac tgttgtggtt
     6241 gctcaatcag cttgggcgac cgcgtgatgt accggtaact aacgtggtgg cactgctggt
     6301 tatgttggta acaaccttgc cgatcctggg ggcctggtgg ctaacccgcg aaggcgacaa
     6361 tggtcaataa ccactgatac aggaatatgc tatgcaacat aagttactga ttaacggaga
     6421 actggttagc ggcgaagggg aaaaacagcc tgtctataat ccggcaacgg gggacgtttt
     6481 actggaaatt gccgaggcat ccgcagagca ggtcgatgct gctgtgcgcg cggcagatgc
     6541 agcatttgcc gaatgggggc aaaccacgcc gaaagtgcgt gcggaatgtc tgctgaaact
     6601 ggctgatgtt atcgaagaaa atggtcaggt ttttgccgaa ctggagtccc gtaattgtgg
     6661 caaaccgctg catagtgcgt tcaatgatga aatcccggcg attgtcgatg tttttcgctt
     6721 tttcgcgggt gcggcgcgct gtctgaatgg tctggcggca ggtgaatatc ttgaaggtca
     6781 tacttcgatg atccgtcgcg atccgttggg ggtcgtggct tctatcgcac cgtggaatta
     6841 tccgctgatg atggccgcgt ggaaacttgc tccggcgctg gcggcaggga actgcgtagt
     6901 gcttaaacca tcagaaatta ccccgctgac cgcgttgaag ttggcagagc tggcgaaaga
     6961 tatcttcccg gcaggcgtga ttaacatact gtttggcaga ggcaaaacgg tgggtgatcc
     7021 gctgaccggt catcccaaag tgcggatggt gtcgctgacg ggctctatcg ccaccggcga
     7081 gcacatcatc agccatgccg cgtcgtccat taagcgtact catatggaac ttggtggcaa
     7141 agcgccagtg attgtttttg atgatgcgga tattgaagca gtggtcgaag gtgtacgtac
     7201 atttggctat tacaatgctg gacaggattg tactgcggct tgtcggatct acgcgcaaaa
     7261 aggcatttac gatacgctgg tggaaaaact gggtgctgcg gtggcaacgt taaaatctgg
     7321 tgcgccggat gacgagtcta cggagcttgg acctttaagc tcgctggcgc atctcgaacg
     7381 cgtcagcaag gcagtagaag aggcgaaagc gacagggcac atcaaagtga tcactggcgg
     7441 tgaaaagcgc aagggtaatg gctattacta tgcgccgacg ctgctggctg gcgcattaca
     7501 ggacgatgcc atcgtgcaaa aagaggtatt tggtccagta gtgagtgtta cgcccttcga
     7561 caacgaagaa caggtggtga actgggcgaa tgacagccag tacggactt
//
LOCUS       DAJVRF010000171         7554 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJVRF010000171 DAJVRF010000000
VERSION     DAJVRF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7554
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.171"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..594)
                     /locus_tag="OQN13_003549"
     CDS             complement(<1..594)
                     /locus_tag="OQN13_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1616815.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIE"
     gene            complement(688..2835)
                     /gene="fdhF"
                     /locus_tag="OQN13_003550"
     CDS             complement(688..2835)
                     /gene="fdhF"
                     /locus_tag="OQN13_003550"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2416..2418),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCS1616816.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVTERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(3033..4499)
                     /gene="mdtP"
                     /locus_tag="OQN13_003551"
     CDS             complement(3033..4499)
                     /gene="mdtP"
                     /locus_tag="OQN13_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCS1616817.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(4496..6547)
                     /gene="mdtO"
                     /locus_tag="OQN13_003552"
     CDS             complement(4496..6547)
                     /gene="mdtO"
                     /locus_tag="OQN13_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCS1616818.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(6547..>7554)
                     /gene="mdtN"
                     /locus_tag="OQN13_003553"
     CDS             complement(6547..>7554)
                     /gene="mdtN"
                     /locus_tag="OQN13_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCS1616819.1"
                     /translation="PRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYASADTIDVV
                     PEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQIMLTQRSVD
                     AQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTAQRAAEADL
                     NAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSV
                     GQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSI
                     GYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEP
                     Q"
BASE COUNT         1701 a         2083 c         2022 g         1748 t
ORIGIN      
        1 ctcgataaaa ccttcttcac cgtttaagaa catcattccc gcccagtact cggaataacc
       61 ggttcgggaa attgcagagc tgcgtttgaa ataccaggtt gccttgtcat cgtcggcctt
      121 aatgcccacg ccgttggcgt aaatcaaacc aagcagcatt tgggcatcga cggcagagtc
      181 gttctccaga tcttccgagg cgttttctaa cagcgaaata gcctttggat aatccggttt
      241 acccgcttga gtatttacca gaatatgcgc cagcgttact tcaccttctt tactgcccgc
      301 ttgcgccgct ttctctgcaa gaacttttgc ttgtggatag tccagactga ccggattggt
      361 gattttgatc tgcgccagca gcgcgcaggc atcagcatca ccgctgtcgg cggctttctg
      421 tgcccaatat tcggctttgc tcaaatcgcc ggagctaaac cagctgtcgg caagaaaata
      481 ttgtgcgcgt cggtccccgg cctctgctgc ctttaaatac tggctgccag gctcggagtc
      541 gttggcgtgg gcaaagaatg tcaaaaacaa catcaatgcg ataatttttt tcattttgat
      601 aatacgattg ggtagggagt aaccagtata aagagttcga atggataaaa aaacagcctc
      661 cgaaaggagg ctgtagaaag gactgtatta cgccagtgcc gcttcgcgca ggcgagtttt
      721 caacttgttg tactcgtcaa tcacgtactg ctcggcggcg cgctgatcgg cgatcggctc
      781 gacgcgcacg gcgcagtatt tgtattccgg cgttttcgta atcgggctta agttttcggt
      841 aaccagctcg ttacaggcac caatccacca ctggtaggtc atgtaaatcg cccctttgtt
      901 cggacgatcg ctgacctgcg cacgggtgat aattttgcct ttacgcgagt gcacccaaac
      961 caatgcctca tcttcaatac ccagacgttt ggcgtcttcg gtattgattt gtgcgtagcc
     1021 aggttcatca gccagcgccg ccagtgccgc acagttaccg gtcatcgaac ggcaagagta
     1081 gtgaccaact tcacgcaccg ttgacagtac catcgggtac tcgtcggtga gtttgtcgat
     1141 tggcgctacc cagtcgcagg tgaagaactg cgccagaccg ttcggggtat caaacttctc
     1201 tttaaacaga taagaagtcc cctgatcggc atctgaagta tcgcggcaag gccactgaat
     1261 gaagcccagt tcgcccattt tctcgtaagt cgcaccgtag aaatccgggc acagatgacg
     1321 caactcatcc cagatctcct gggtgttgtt gtagtgcatc ggataaccca tacgggtggc
     1381 gatttcactg atgatttgcc agtccgtttt cagatcccat ttcggttcaa ctgctttgaa
     1441 gaaacgctgg aagccacggt cagccgcagt aaacacgcct tcatgctcgc cccacgacgt
     1501 tgacggcaaa atgacatccg ccgccgacgc ggttttggtc ataaagatgt cctgaacgat
     1561 aaccagttcc agatcttcaa aggctttacg tactgccgac agctccgcgt cagtttgcag
     1621 cggatcttcg cccataatgt acgcggcacg cacttcgcca tgcgctgcgc ggtgcggcag
     1681 ctcgctgatg cgataaccgg tatgagcagg caggctttcc acgccccagg ctttggcgaa
     1741 tttctcgcgg ttagccggat ctttcacgta ctggtatccc ggatacgtat ccggcagcgc
     1801 gcccatatcg caggcaccct gcacgttgtt ctgaccacga accgggttta cacccgcatg
     1861 cggcttaccg aggttaccag tcagcatcgc gaggctggtc agagaacgca cggtttccac
     1921 gccctggtag aactgggtta cacccatgcc ccacaggatg gcggcgcttt tcgcctgggc
     1981 atacatccgt gccgcctgac gaatctcact ggcgctgacg ccggtgatat cttcaaccga
     2041 ctccggcgtg tagccttcaa cgattttacg atactcttca aagccttctg tacgggaagc
     2101 gacgaacgct ttgtcgtaca gattttcttc aataatgaca tggcccatcg cattcaacag
     2161 cgcgatgttc gagccgtttt tcagtgcaat gtgcatgtca gcaatgcgcg cggtttcaat
     2221 tttgcgcgga tcgcagacga taattttcgc cccgttacgt ttagcgttaa ttacgtgatt
     2281 cgccacgatt gggtgggaat cagccgggtt gtacccgaaa acgaacacta aatcggtatt
     2341 atcaatttcg ttaatagcat tgctcattgc gccattaccg accgattggt gcagacctgc
     2401 aaccgatggg ccgtgtcaga cacgagcgca gcagtcaacg ttattggtac caataacggc
     2461 gcgcgcaaat ttttgcatta catagttggt ttcgttaccc gtaccacgcg aggagccggt
     2521 cgtctggatg gcatccggac cgtacttctc tttgatggcg ctcaggcgct cggtaacgta
     2581 attcagtgcc tcatcccagg aaacaggttc gagtttgccg ccacgctggc gacggatcat
     2641 gggggttttc aggcgcgggg tcaggatctg ggtatcgtta atgaaatccc agccataata
     2701 acccttcaga cacagggtac cctggttggt tttcccctgc gctgcctccg cccggacgat
     2761 tttgccgtta tcgacgacca ggttgatttt gcaacctgat gcgcaatagg ggcaaaccgt
     2821 gacgactttt ttcatcggtc tcgctccagt taatcaaatc acgcatacgc gctctcgact
     2881 acagtatgca tcttttatgc cacattttat ttggggtcat tccctgatat tacgggcact
     2941 atttattcaa aactctgacg aaaaacaggc tgtcgtcagt tttgacgtga cgaaacgaaa
     3001 taccgcgtga cagccatcac gcggcagaca ttttattttt tctcgacgac gggagccgcc
     3061 tgatacccgc cgcccagcga tttcatcaac tgaatgcttt ggatcacccg gcggctgtcc
     3121 agcatcagta atgacatctc ttcggcaagt actggcaacc gggcttcagt ggcctgtaag
     3181 cggctggtta agccgcgctg ataggcggcc tcggcagcgc gctgggtgaa gcgcgttgct
     3241 tccacgcgtt cagcctgcat ttctcgctcg tcgttgagcg tttgcagacg cgtgccgttg
     3301 acggcaacgt cacgcaccgc gttcagtact gactggttgt aacgttcaat catcatgttg
     3361 ctggcggcgc gcgtgccttc gagattggca ttcaaccgtc caccgtcaaa cagcggcaat
     3421 ttcagacccg ggatgaagtt gaactggcga ctggtttttt tgaataaggt atccaggtgg
     3481 atggcatcaa ggccgaaaaa cgctttgata tcaaagctcg ggtagaacag cgcccgcgcg
     3541 gaatccacct gatctaatga cgcctgaaca taccagcgca tggcttgcag atccgggcgt
     3601 ctggcgagca actcataaga gagtgtcgcc ggaatgccgg tctggactcg tggtaatgcc
     3661 accggtttga tctccggcat atcgctggcc cccgcgccaa tcaatgcacg cagagattct
     3721 cgcgtttcgg tgatttgccc tttgacggca gcaatttgtt tatcgaccgc cagaatctgt
     3781 gcccgcgcgc cgtggaaagg cacttgtgct tccagaccgt gcgccacttt actctggtgc
     3841 gctttcaccg cgtaatcaat cacatcgcga gtttgttcta acagatcgag catctgatag
     3901 ctggcctgca tactgtaata aagctgcgct acgcccgtgg tcagcgatag ctctactgct
     3961 gcggtttctg ccagcgcggc attatgcgcg ccaatggcgg cggcaaccgc tgagcgatgc
     4021 acaccccaca aatcaagatc cagtccggca aacaaaccta ctgtggcttc cgtatagtac
     4081 ggcccgtcca tacccagtgc gggcgcatcc atcgcataag ggcttaaaaa gccgttcgcc
     4141 gagacgcgtt ggcggttgag catccctaac gctgcgacct gtaattgtga accggcatct
     4201 aacaaatcgg cctgggactg cgctttttct tcccgcagtt tcgcttcggc gagggtgtgt
     4261 gaaccactta gcgtccgttg gatcagcgca tccagctgcg ggtcattgag ttgtttccac
     4321 cactgcgcct gcggccagcc ggagctggca agatgaatat catcggccag tttgatttgt
     4381 tccggtttga gctgttgatg aggcgcagaa tccttgcgta ccaaggcaca gccggaaatc
     4441 agcgtcgtgc tgccgagaat gctgcacaac agcagacgtg aaagttgacg attgatcatt
     4501 gcgtggctcc ttgtgcctgt tccgtggcgg gcgttaatgc cggggctgtc cagtctggca
     4561 agcgggcgaa aagctggcag acgtgttgct cctgttttaa taaggtgggc aggatggcct
     4621 gcgaggcggg tgtctcttca agcgttattt gaggagaacg gctgagtgcg gttgccagac
     4681 cggcagcgta tttttccagg gcgtcggcaa actgacctgc tctgtccggc tgtagtgcgt
     4741 tatccagcgc ctgcgccgaa ttccaggtgg catcccaggc gtgaaggata tcgcggccct
     4801 gacgaataac cgtttgcgaa cgttcaatca gtaaggcgcg ttcttcgctg tccagttgac
     4861 gctccagcgc cacgcgttgg cacatctctt cacaggcatt aaacgccgca tgcagaccga
     4921 tacgaatttg cagataagtg cgcagagccg tgacttcctg ctggcgtggg atccgcatta
     4981 ctttacttaa catacccagc gcgccagcca gtttttgcgg cagcgtgcgc gcttcacttt
     5041 caggccagac aaaggtgtaa atcaccgcgg acaccacggt accaatgatg atacccaggg
     5101 cgcgatcgcg aatttccacc aggtcgtaca ctgggccaaa aacgttttcg agcgtggcga
     5161 gcgcgaaggt gaccaccatc tgtgtgccga tataagaaga gcgttcagag ctggtggcaa
     5221 tccatgcgcc caacaggaaa atcggtgcca gcacaaacag caattcgaca atattgtcca
     5281 gccagggcat gaccagtagc gtgaataaca gcgccagaat cgcgccgcaa aaggccccgc
     5341 caaaacgcag caccatcttc tggtacgacg aaccgacatt tggattagag acgatcacgc
     5401 aggtcagcat acaggtgtga atgccttccc agtccacgcc gctgtaaaag gtgtaacaga
     5461 tcaaacaggc gagcaatgtt tttaccgcgt agcgtatata gtctggattg gtaaaagcat
     5521 cggcgaccat tgatggcggt ttggcggcgg gcgttggcgg cgtattcggg ttcatctggc
     5581 ccagttgtaa caacgtctgg cagatattct ccaggttaca ttcccgtgcc gccaccgctt
     5641 cactttcact gatccgccag tcgctttgcc agcactgacc ttcagcaacg gcatgctgca
     5701 gcttgttgat ttctgaggct aatttttgcc gaaattcaat aattgcctga gaatcagcaa
     5761 aagaggtggg atcgtagcga ttcagcgtcg agtaaatgta ggttaccgtt gccacgcagc
     5821 tttgccacca tgcgctttga gttcgccagt tggcatcgtc cgcgagacaa aagacattca
     5881 gtttttgcag cgccagcgcc tctctttcaa tccgcgtttc gggtagcggt gcgaggctgt
     5941 ctgtcaggtg actaatggca tcatcaagcc gatcattaag cgcctgatgc atttgcgaaa
     6001 tggcacgact gggaaaccac agcacgccga ttaacgtcat cagcaaggtt ggatagaggc
     6061 caacaacgat acaccacagc gttaagcgca cgaccacttc cggatagtcg agcatggcgg
     6121 ggaaggtttg cccgtaaatg gcgacaatgg cgacagcgaa aaagaccagt cccagccgat
     6181 gggtgcgcat caaaaacatg cggcccatca ggatcggccc ggcgatgatc aatcggatca
     6241 acggttcgcc gtatgaccat ttgtagatca aaaacaggct gccgatctcc agcaccgtgg
     6301 caaccacaaa caagatcgcc acaaatttgg tgtaaaacgc gttcgactga ataccgtaaa
     6361 acagcactgc cagcgataac gccacaaaag ggatctcaaa agtcatcgag atcagaatca
     6421 ccagcaggca gcctacccag agttgcacga tctgcggcac gcggccgggt cgccgctcgc
     6481 ttaactcttc atgaaagaac gccagcagcc tgaccatcgg taatggcagg gagttgagcg
     6541 cgctcattat tgcggctcaa gattagcgac tgccgaagcg ccgatgcgga acatttccgg
     6601 gtcaggttta tcgaccatga ttttgaccgg aaaacgctgg gcaacgcgga cccagttaat
     6661 agagcgagaa actttcggca ggccgcccag caccaggccg ccgtcatccg gtagcacgcc
     6721 gtagccaatc gaatccactt taccctcgaa ggttttgccg ctgtcactca tcaggcgaat
     6781 cgttgcgggt gtacctgagc gaatattttt cagatcggtt tcgcggaagt tggcgatcac
     6841 gtaccagtga cgagtgtcga ttagggtaaa aatagggcgc atggcagaag caaattgccc
     6901 gacggaggtt ttgagggaaa tgacccggcc atcaaacggc gcgcgaacgg tcgccatttc
     6961 cagatgcagt ttggtcaggg caatatccgc ttcgaccgcc gcacgttgcg ccaccagcgc
     7021 atcgacgccg ctgacggcgc tggcggctga ctgcgcctgt aacaatacgg cattaagatc
     7081 ggcttctgcg gcgcgctgcg cagttcttgc acggtcaacg tcttccgctg agacaaaacc
     7141 ctctttcagt aatggctcgg tacggcgtaa tgtatctgtg gcctgtttcg cggcggcacg
     7201 ggctttttct accgtggcat taatcgagtc ggcaccaaac tgttgcgcat caacgctacg
     7261 ctgggtgagc ataatttgct tatccagcgc cgcgagggag gcttcagctt tcgccagatt
     7321 ggcttcgtac gggcgcgggt caatgcggaa cagcaaatcg ccctgtttga ctgcctggtt
     7381 gtcggtgacc gccagttcta caatgcggcc gctgacttcc ggcaccacat caatggtatc
     7441 tgctgacgcg taagcgtcat tagttgatgg cgcactgtct acgcgccaga taacgaaaac
     7501 aagggcaacc agcgccaatg caaccactaa cagagcaggg aatttactgc gagg
//
LOCUS       DAJVRF010000172         7433 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJVRF010000172 DAJVRF010000000
VERSION     DAJVRF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7433
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.172"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..239
                     /locus_tag="OQN13_003554"
     CDS             <1..239
                     /locus_tag="OQN13_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HCS1616820.1"
                     /translation="LLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIVQLLLEHG
                     ASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            279..1142
                     /locus_tag="OQN13_003555"
     CDS             279..1142
                     /locus_tag="OQN13_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCS1616821.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            1132..2679
                     /gene="fdrA"
                     /locus_tag="OQN13_003556"
     CDS             1132..2679
                     /gene="fdrA"
                     /locus_tag="OQN13_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCS1616822.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            2679..4037
                     /locus_tag="OQN13_003557"
     CDS             2679..4037
                     /locus_tag="OQN13_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCS1616823.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAGPPITWEKMCGAMKGAVTGAL
                     VFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGVTSASMFMHIVKNKTYGNIAY
                     TNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDAMKIIGEIDLRLMLAQALHMG
                     DECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEFVASSDYFSGPTWMAMCKAAM
                     DAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPAQQVIGPMFAGYKPEDSGLDI
                     GDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSRQMREITLGENPNVTIPLLGF
                     MGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAGIVHPPFACFEKAILGWCERY
                     GV"
     gene            4180..5130
                     /locus_tag="OQN13_003558"
     CDS             4180..5130
                     /locus_tag="OQN13_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HCS1616824.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADMVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            5140..6522
                     /locus_tag="OQN13_003559"
     CDS             5140..6522
                     /locus_tag="OQN13_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCS1616825.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            6899..>7433
                     /gene="yahK"
                     /locus_tag="OQN13_003560"
     CDS             6899..>7433
                     /gene="yahK"
                     /locus_tag="OQN13_003560"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HCS1616826.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKV"
BASE COUNT         1779 a         1903 c         2160 g         1591 t
ORIGIN      
        1 agcttttggc gcatacggag attaacgtta accagaccaa ccatgtaggc tggacgccgc
       61 tactggaagc gattgtgctt aatgatggtg gtattaaaca gcaggcgatt gtgcagttat
      121 tgctggaaca cggtgccagc ccgcatctga ccgataaata tggcaaaacg ccactggaac
      181 tggcgcggga acggggcttt gaagagattg cgcagttact gattgccgca ggtgcataaa
      241 ccgggaggct ggctatcaac acaccagaaa aacggtgtgt gtgggcgcta actgcggatg
      301 cggattttct ggcgcagcgg gggcaaggac aggttgaaca ggtctttgcc agagcggtaa
      361 atatcgcact cccggctcgc cagcagttgc tgacgctgct ttgtgaagag tacgacaatg
      421 cgccaaacag ttgtcggttg gcactcactc actttgatga tctgttccgg catggtgata
      481 aggttcagtt tgacgatcaa ggtattacgg ttggtcaaca tcttcatata gagatgagtc
      541 gttgtcggcg ttggctgtcc ccaaccttgc aaatgaccgc tgtgaatttt caccttatcg
      601 cctggctaca gtggcacgac attattcatc agcacctggg ggaaaatgaa accctgttta
      661 attatcgcgg cgataatccg ttttatcagg cgttaaataa agaattacat attaaacgac
      721 gggcagttat tcagacagta aacgataagc aaaatatcgc cgcagcggtc gccagtatga
      781 ttgggttagg gattggcctt acgccatcag ccgatgatta tttaacaggt ctggcgctta
      841 ttttatttat tcccgggcat ccggcagaaa aatacaaaga ggaattttat cgcggtctgc
      901 tacgcggcag aaataacacc acgttattaa gtgccataac gctggaagcc gcattacaac
      961 aacgctgccg ggaaaatatt catcgcttta ttcacaacat tatttatggc gtccctggga
     1021 attcaactca ggcaatagaa aaaattaaac atattggctc cagttccggc tgcgacatgc
     1081 tgtatggcat ggccgatggt tgtgcgctga gccaaaccta cggagggaat tatgtcagtt
     1141 aaaatagtca ttaaaccgaa tacctatttt gattctgtct cgctgatgtc tatctccacg
     1201 cgtgcaaata aactcgacgg cgtcgagcag gcatttgtgg cgatggcgac cgaaatgaac
     1261 aaaggtgtgc tgaaaaattt aggactgctg acgccgacgc tggaacaggc gaaaaacggc
     1321 gacctgatga ttgtcatcaa tggtaaatcg ggtgcggaca acgagcagtt gctggtggag
     1381 attgaagaac tgttcaacac caaagcgcaa agcggctcgc acgaggcgcg ttacgccact
     1441 attgccagcg ccaaaaagca tatcccggaa agtaacctgg cggtgatttc ggtcaacggt
     1501 ctgtttgccg ctcgcgaagc gcgtcaggcg ctgcaaaacg atctcaacgt gatgctgttt
     1561 tccgataacg tctcagttga agatgaactg gcgctcaagc agttggccca cgaaaaaggg
     1621 ctgctgatga tggggccgga ctgcggcacg gcgattatta acggcgcggc gctctgtttt
     1681 ggtaacgccg tgcgtcgcgg caatatcggt attgttggcg catccggcac cggcagccag
     1741 gagctgagcg tccgcattca tgaatttggt ggcggtgttt cacaactgat tggcaccggc
     1801 gggcgcgacc tgagcgagaa aatcggcggc ctgatgatgc tcgacgccat cgggatgctg
     1861 gaaaacgatc cgcaaactga aattatcgca ctgatttcaa aacctcccgc gcctgcggtg
     1921 gcccgcaaag tgctggaacg cgcacgtgcc tgccgcaagc cggtggtggt ctgcttcctc
     1981 ggtcgcgttg aaacgccggt ggatgagcag gggctacagt ttgcccgcgg cagcaaagag
     2041 gcggcgctaa aagcggtgat gctctccggc gtgaaacagg aaaatctcga cctgcatacg
     2101 cttaaccagc cgttgattgc tgatgtgcgt gcgcgtctgc aaccgcagca gaaatacatt
     2161 cgtggcctgt tctgcggcgg cacgctgtgc gacgaaacca tgttcgcggt gatggaaaaa
     2221 catggcgatg tctacagcaa cattcagccc gatccggaat tccgcctgca agatatcaac
     2281 cgcagcatca aacacacctt cctcgacttt ggcgatgacg acttcacgaa cggcaagccg
     2341 catccaatga ttgaccccac caaccgcatc agtcgcttga tcgaagaggc gcgcgatcca
     2401 gaagtggcgg tgatcgtgat ggattttgtg ctcggatttg gatcgcatga agatccggtc
     2461 ggctccacca tcgaggcgat caaagaagcg aaagcgatcg ccgctgccga aggacgcgag
     2521 ttgatcattc tcgcctatgt gctgggtacc gatcttgata cgccatcgtt agaacaacaa
     2581 agccagatgc tgcttgatgc cggagtgatt ctggcgagca gcagcaccaa taccggattg
     2641 ctggcgcgtg aatttatctg caaaggggag gaagcctgat gagccagtca ctgtttagcc
     2701 aaccattgaa cgttattaac gtcggcatcg ccatgtttag cgatgacctg aaaaagcagc
     2761 atgtagaagt gactcaactc gactggacgc cgccggggca gggcaatatg caggtggtgc
     2821 aggcgctgga gcgcattatc cagtcgcatc cggtgctgat tggttttgat caggcgatta
     2881 acgtggtgcc gggcatgacg ccgaaaacca ttcttcacgc cgggccgccg atcacctggg
     2941 aaaaaatgtg cggcgcgatg aaaggcgcgg tcaccggagc gctggtgttc gaaggactgg
     3001 cgaaagatct cgacgaggcg gctgaactgg cggcctccgg ggagatcacc ttctcgccgt
     3061 gccacgaaca cgactgtgtg ggatcgatgg cgggcgttac ctcggcctcg atgtttatgc
     3121 acatcgtgaa aaacaaaacc tacggcaaca tcgcttacac caatatgagc gagcagatgg
     3181 cgaagatttt gcgtatgggt gccaacgatc agagcgtgat cgaccgcctc aactggatgc
     3241 gcgatgtgca ggggccaatg ctgcgcgacg cgatgaaaat tatcggtgag atcgatctgc
     3301 gcttaatgct ggcgcaggcg ctgcatatgg gcgatgagtg ccataaccgc aataacgccg
     3361 ggacgacgct gctgattcag gcactgacgc cggggatcat tcaggcaggc tattccgtcg
     3421 agcaacagcg cgaagtgttc gagtttgtcg ccagcagcga ctacttctcc ggcccgacgt
     3481 ggatggcgat gtgtaaagcg gcgatggatg cggcgcacgg catcgaatac agcaccgtgg
     3541 tcaccaccat ggcgcgtaac ggcgtcgagt tcggcctgcg ggtcagcggc ctgccggggc
     3601 aatggtttac cggcccggcg cagcaggtga tcggcccgat gtttgccggt tataagccgg
     3661 aagattcggg gctggatatc ggcgacagcg ccatcaccga aacctacggt attggtggat
     3721 ttgctatggc gaccgcgcct gcaatcgtcg cgctggtggg cggcacggtg gaagaggcta
     3781 tcgatttctc ccgtcagatg cgtgaaatca ctctcggtga aaaccccaac gtcaccattc
     3841 cgctgctcgg ttttatgggc gtgccgtcgg caatcgacat cacccgcgtg ggtagcagcg
     3901 gcattctgcc ggtgatcaac accgccatcg cccataaaga tgcgggcgtc ggcatgattg
     3961 gcgcgggcat tgtgcatcca ccattcgctt gcttcgagaa agccattctc ggctggtgcg
     4021 aacgttacgg cgtctgactt acatgtttat gccggatgcg gcgtgaacgc cttatccggc
     4081 ctacggatgg cccgagaatt gtaggcctga taagcgtagc gcatcaggca gtctggcgtt
     4141 ggtcataacc tcatcaccct ctgtagcgga cataacaaca tgaaagagct tgtggtcgtt
     4201 gccattggtg gcaacagcat tatcaaagat aacgccagcc agtcgattga gcatcaggcg
     4261 gaggcggtga aagccgtggc cgatatggtg ctggaaatgc tggcttccga ttacgacatt
     4321 gtgctgaccc acggtaacgg gccgcaggtc gggctggatt tacgccgtgc ggagattgcc
     4381 cacgagcgcg aagggctgcc cttaacgccg ctggcgaact gtgtggcaga tacgcagggc
     4441 ggcatcggct atctgatcca acaggcgctg aataaccgac tggcgcgtca cggcgagaaa
     4501 aaagccgtca ccgtggtaac tcaggtggaa gtggataaaa acgatccagg ttttgcccat
     4561 cccaccaagc ccattggtgc attcttcagt gaaagccagc gtgacaaact acaaaaggca
     4621 aaccctgact ggtgttttgt tgaagatgcc ggacggggct atcgccgcgt ggtcgcctcg
     4681 ccggaaccga aacgtattgt cgaagcaccc gccattaagg cgctgatcca acaaggtttt
     4741 gtcgtaattg gcgcgggcgg cggtggaatt ccggtagtgc gtactgaagc gggggattac
     4801 caaagcgtgg acgcggttat tgacaaagat ctctctaccg cgctgctggc ccgtgaaatt
     4861 cacgccgaca ttctggtgat caccaccgac gtcgaaaaag tctgtatcca ctttggtaaa
     4921 ccgcagcagc aggcgctgga tcgggtggat attgccacca tgactcgcta tatgcaggaa
     4981 gggcattttt ctcccggcag catgttgcca aaaatcatcg ccagcctgac gttcctggag
     5041 cagggcggca aagaagtgat tatcaccacg ccggaatgcc tgcctgcggc gctgcgcggt
     5101 gaaacgggca cccatattat taaaacgtaa ggacgtagga tgaaagaaag caatagccgc
     5161 cgtgaatttc tgagccagag cggtaagatg gtcaccgccg ccgcgctgtt tggtacctat
     5221 gtgccgctcg cccatgcggc ggtagctggc accctaaact gcgaagcgaa caacaccatg
     5281 aaaatcactg acccgcatta ctatctcgat aacgtgctgc tggaaaccgg ttttgactac
     5341 gaaaacggtg tggcggtgca aacccgcacg gcgcgccaga ccgtggagat tcaggacggc
     5401 aaaattgtcg ccctgcgcga gaacaagcag catccggacg ccacgctgcc gcactatgac
     5461 gctggcggta agttgatgct acccaccact cgcgacatgc atattcatct cgacaaaacc
     5521 ttctacggcg ggccgtggcg ctcgctcaat cgtccggcag gcaccactat ccaggacatg
     5581 atcaaactca agcagaaaat gctgccggag ttacaaccgt atacgcagga gcgggcggaa
     5641 aaactgatcg atctattgca gtcgaaaggc accaccattg cccgcagcca ctgcaatatc
     5701 gaaccggttt ccggcctaaa aaatctgcaa aatttgcagg cggtgctggc gcggcgtcag
     5761 gcgggcttcg agtgtgaaat cgtcgccttc ccgcagcacg gtttgctgct gtcgaaatcg
     5821 gaacccttaa tgcgcgaagc gatgcaggcg ggtgcgcatt acgtcggcgg gctggacccg
     5881 accagtgtcg atggcgcgat ggaaaaatcc ctcgacacca tgtttcagat tgcgctggac
     5941 tacgacaaag gtgtcgatat tcacctgcac gaaaccactc cgtcgggcgt ggcagccatc
     6001 aattatatgg ttgaaacggt agagaaaacg ccgcaactga aaggtaagct gaccatcagc
     6061 cacgcctttg cgctggctac gctcaacgaa caacaggtcg atgcgctggc gaatcggatg
     6121 gcggcgcagc aaatttctat cgcctcgacg gtgccgattg gcacgctgca tatgccgctc
     6181 aaacagttgc acgacaaagg cgtaaaagtg atgactggca ctgacagcgt tatcgaccac
     6241 tggtcgcctt atggtctggg cgacatgctg gaaaaagcca atctctacgc gcagctctat
     6301 attcgtccta acgaacagaa cctctcccgt tcgctgtttc tagccactgg cgatgtattg
     6361 ccgctgaatg aaaaaggcga gcgtgtatgg ccaaaagcgc aggatgatgc cagctttgtg
     6421 ctggtggacg cctcctgttc cgccgaggcg gtggcgcgta tctcgccgag aaccgcaacg
     6481 ttccataaag ggcaactggt gtgggggagt gtggcaggtt gatgcgcatt tgaaacgcct
     6541 gcaacggtga attgtaagag atttaagccc cagggagtgg ggcgacctca atatgatagt
     6601 gtcggcagaa atggacatta tctttgagga atatgccctt attgacgttg tgtagataac
     6661 tatttgtcta cgtgaaaaga tcatcagttt tgccgatttt agtcttttac ggatagcaaa
     6721 tatcacactt ataggcgctc gccttatggg gaacgttgct gctctgtttc attcaggttc
     6781 gcggcatgtt ggggctggat cgggtggttt aaatattggc tatattcaat ggacgcgttt
     6841 tgccgcgatg acatatcagg cgtcgccaaa tacacatagc taatcaggag taaacacaat
     6901 gaagatcaaa gctgttggtg catattccgc taaacaacca cttgaaccga tggatatcac
     6961 acggcgtgaa ccgggaccga atgatgtcaa aatcgaaatc gcttactgtg gcgtctgcca
     7021 ttccgatctc caccaggtcc gttccgagtg ggcggggacg gtttacccct gcgtaccggg
     7081 tcatgaaatt gtggggcgtg tggtagccgt tggtgatcag gtagaaaaac atgcgccggg
     7141 cgatctggtc ggtgtcggct gcattgtcga cagttgtaaa cattgcgaag agtgtgagga
     7201 cgggctggaa aactactgtg atcacatgat cggcacctat aactcgccga cgccggacga
     7261 accgggccat actctgggcg gctactcaca acagatcgtc gttcatgagc gatatgttct
     7321 gcgtattcgt cacccgcaag agcagctggc ggcggtggct cctttgttgt gtgcagggat
     7381 caccacgtac tcgccgctac gtcactggca ggccgggccg ggtaaaaaag tgg
//
LOCUS       DAJVRF010000173         7214 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJVRF010000173 DAJVRF010000000
VERSION     DAJVRF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.173"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..99)
                     /locus_tag="OQN13_003561"
     CDS             complement(<1..99)
                     /locus_tag="OQN13_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HCS1616827.1"
                     /translation="MTLTRREFIKHSGIAAGAQVVTSAAPLPAWAEE"
     gene            complement(124..1224)
                     /locus_tag="OQN13_003562"
     CDS             complement(124..1224)
                     /locus_tag="OQN13_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCS1616828.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKSFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(1612..2358)
                     /gene="cutC"
                     /locus_tag="OQN13_003563"
     CDS             complement(1612..2358)
                     /gene="cutC"
                     /locus_tag="OQN13_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCS1616829.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(2372..2938)
                     /locus_tag="OQN13_003564"
     CDS             complement(2372..2938)
                     /locus_tag="OQN13_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1616830.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            3154..4890
                     /gene="argS"
                     /locus_tag="OQN13_003565"
     CDS             3154..4890
                     /gene="argS"
                     /locus_tag="OQN13_003565"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCS1616831.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            5067..5429
                     /locus_tag="OQN13_003566"
                     /pseudo
     CDS             5067..5429
                     /locus_tag="OQN13_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
     gene            complement(5433..6503)
                     /locus_tag="OQN13_003567"
                     /pseudo
     CDS             complement(5433..6503)
                     /locus_tag="OQN13_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            complement(6496..>7214)
                     /gene="flhB"
                     /locus_tag="OQN13_003568"
     CDS             complement(6496..>7214)
                     /gene="flhB"
                     /locus_tag="OQN13_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HCS1616832.1"
                     /translation="LLKAILKTILVGSVTGFYLWHHWPQMMRLMAESPTTAMGNAMDL
                     VGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQSEGDPHVKGRIRQM
                     QRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVAKGAGLVALRIREIG
                     AENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQLKRWRLAGGQRPVQ
                     PTHLPVPEALDFINEKPTHE"
BASE COUNT         1868 a         1881 c         1718 g         1747 t
ORIGIN      
        1 ctcttctgcc catgcgggga gcggtgctgc cgatgtcacc acctgcgctc cagcggcaat
       61 accgctgtgt ttaataaatt cacgtcttgt taatgtcata acttcctccc tgatcaacga
      121 ggatcactgt ttctcggtaa tatctttggc gttgtactgg aaataccgcg ttaaaatgtc
      181 cagttcgttt tcgctcatgc tggttcgtgc ccccattcct ttggcaatgg acggccacgc
      241 attgacggtg taatggtcgg cggcaatagg ggcatgacaa ccagcgcaat aggtatcgtc
      301 aagtttttca gcgtattgcc atagcggttt acggtccgct aatccgggat cggtaagcgc
      361 accctgtaaa gacgcctgac gccattgatt gccgtattcg tcagcctgcc attccccgtt
      421 taccgtgagc gccttgatac cttcttcact taatgtggct agcgccagcc gttgacctgc
      481 cgccaggtag agcgtgtttt cactgccctg catttgataa ccctgcaaca gaacgatcgg
      541 ctgtttgcca ctggcatcaa cgacggtgag atcggtacca ggattcacgg tagccagctc
      601 gcctatgcga gaagttttga aaggataaat atgtgcgcca ttagtcactg aagtagcggc
      661 ctgactttcc agctcatgcg ccgcgttgtc atccattttt atttctggcg gaaaatgggc
      721 aatgccttta tgacaatcga tacaggtttc gccgcccttt tgtgctttgt tatgcatttt
      781 ctgcgcagat tcactttgcg aggcaatatc catggcatca aaagaatggc aactacggca
      841 cgttgcagag tcagttgctt ttaattcttt ccatactgtt tcggccattt cctggcgatg
      901 agtttcgaac ttatcgtcac tgtctatttt gccgctaaca aattcatgat aaatatcttt
      961 agatgctttt aatttagcaa ataaataatc catccctgac tttggaatat ggcaatctgc
     1021 acattccgca cgtatccctt tctggttcga aaagtggaca gttccctgat attcctcaaa
     1081 agatttactc atcgagtggc aggaaaggca aaatgctgta tccgacgttt tatgtaaggc
     1141 tttttgcgcc agcaataacc cgccgccacc aaccacaacc gctatcagca aaaacaataa
     1201 cccaatgcgt tttttccctc gcatagattc ctctaaataa attccagaac accggaatca
     1261 ttgtataatt aaatacacaa cgaattaacc gcttgcataa ctcggcacaa cactgcctga
     1321 aataatcgat aaagaatatg atttattaca atgtaatcat taattgctaa ggaataaccc
     1381 agttgctatt tagaatatat atttatcagt tttagcaatt taaatcccat aatcaatgtg
     1441 aatataaaaa atattattag cacaccgcta tgttcactgt cacccgctca acagatcacg
     1501 gttatcgttc gttttttata ctgctcaggg ataaaaaaag gcccctgttg aaattgcagg
     1561 ggcctggtac gagcaagcat catattgggc gacatgatgc aacggtaaaa atcatttggc
     1621 ctgatggcgt tcaatgattc ctttcatttc agcaaccgcc gccccgtcta cgatataacg
     1681 cgaatactcg tccgcgtgtt catctgatga catggacaat ccttgattac gataacgcat
     1741 cggtgaggct tgccacgctc ccgcggagct atggacttcc agcactccgg catcgaggaa
     1801 gtggtgcagg ttttctgcac ggactcctgc tccggccata atgattggag catcacgatg
     1861 ggcaataagt tccataattt ttgataaacc ttgcagtgcg tctgattttt gccctgatgt
     1921 cagtacccgg gcaatgccca attccgcaag attattgagt gtatataaag ggttagcgca
     1981 catatcgaag gcgcgatgaa aagtcactgc cagcggaccg gcagcagcca ttattttttc
     2041 cattcgtggc atatcgacat tcccgtcaac atcgagaacg cccgtcacca gtccaggaaa
     2101 acctaattcg cggaccgtgc gcacatcctc aagaatggcg gcaaactcac cgtcgctgta
     2161 gcaaaaatca ccaccgcgtg ggcgaattat cggatgcaca gggatcgtca cccgctggcg
     2221 cacggatttc agtaccccca acgacggcgt taagcccccc tcttttgggg ctgcgcataa
     2281 ttcaactctg tctgcgccgt tttgctgcgc cgttagtgca cattccatgc tgtaacagca
     2341 aatttccagt aatgccattt ttgctcctta attacgctga ctgctcgctg gcaacgatct
     2401 cttcaatgga ccacggatga aacttaatag ttgttttgcc atcggtgacc gccagcgtcg
     2461 gattcggtaa tcgctcatgc tcaccttttg gtgaactggt tttaactgag atccccggta
     2521 ggctgagtcc ttcatcagaa agcaatgcca gcgcacgggc attcagcgtt tctggatcac
     2581 ccagcagaac gatttcaata tgttcccaac cttcgtgtgg gtaacgtttt tccccgggcc
     2641 acggtagctc cacaatagaa aactgccagt gcgcaacctg taccggttca tgcaatttaa
     2701 acagacaaat cggtctgcca ttgatcatat tttctgacaa aagctcgcca cactgttcaa
     2761 acccgcgacg ccagcgttca gcagtggcgt tttgatggca acgcaaagaa atgtgatcgg
     2821 cagtcagcgg agtgatattc aaccccagac ggagggaaag ttcatctaat gcgtgggtaa
     2881 atcgcggtaa atccgatgca atatcctgca gctcgtcgat agattgccag ttcgccataa
     2941 tcactcttcg tctttcagta aaagcgttaa tttaccctgt tgccctgcgc caaccaaccg
     3001 ctgatttcac gccgcttctg atgcaatagt gaaaacggca atacgccacg cgcacgttgc
     3061 tgacgaaaac agccatttgc agtatactcc cgccctaatt tctttaactg gtgcgggcaa
     3121 tttttgctcg cttcatcaat gtaaggtatt ccggtgaata ttcaggctct tctctcagaa
     3181 aaagtccgtc aggccatgat tgcggcaggc gcgcctgcgg attgcgaacc gcaggttcgt
     3241 cattcagcaa aagttcagtt cggcgactat caggctaacg gcatgatggc agttgctaaa
     3301 aaactgggta tgacaccgcg acaattagca gagcaggtgc tgactcatct ggatcttaac
     3361 ggtatcgcca gcaaagttga gatcgccggt ccaggcttta tcaacatttt ccttgatccg
     3421 gcattcctgg ctgaacatgt tcagcaggcg ctggcgtccg atcgtctcgg tgtttctacg
     3481 ccagaaaaac agaccattgt ggttgactac tctgcgccaa acgtggcgaa agagatgcat
     3541 gtcggtcacc tgcgctctac cattattggt gacgcagcag tgcgtactct ggagttcctc
     3601 ggtcacaaag tgattcgcgc aaaccacgtc ggcgactggg gcactcagtt cggtatgctg
     3661 attgcatggc tggaaaagca gcagcaggaa aacgccggtg atgaaatgga gctggctgac
     3721 cttgaaggtt tctaccgcga tgcgaaaaag cattacgacg aagatgaaga gtttgccgag
     3781 cgtgcacgta actacgtggt aaaactgcaa agcggtgacg aatatttccg cgagatgtgg
     3841 cgcaaactgg tcgacatcac catgacgcag aaccagatca cctacgatcg tctcaacgtg
     3901 acgctgaccc gtgatgacgt gatgggcgaa agcctctaca acccaatgct gccaggaatt
     3961 gtggcggatc tcaaagccaa aggtctggca gtagaaagcg aaggggcgac cgtcgtattc
     4021 cttgatgagt ttaaaaacaa ggaaggcgaa ccgatgggcg tgatcattca gaagaaagat
     4081 ggcggctatc tctacaccac cactgatatc gcctgtgcga aatatcgtta tgaaacactg
     4141 catgccgatc gcgtgctgta ttacatcgac tcccgtcagc atcaacacct gatgcaggca
     4201 tgggcgatcg tccgtaaagc aggctatgta ccggaatccg taccgctgga acaccacatg
     4261 ttcggcatga tgctgggtaa agacggcaaa ccgttcaaaa cccgcgcggg tggtacagtg
     4321 aaactggccg atctgctgga tgaagccctg gaacgtgcac gccgtctggt ggcagaaaag
     4381 aacccggata tgccagctga cgagctggaa aaactggcta acgcggttgg tattggtgcg
     4441 gtgaaatatg cggatctctc caaaaaccgc accacggact acatcttcga ctgggacaac
     4501 atgctggcgt ttgagggtaa taccgcgcca tacatgcagt atgcttacac gcgtgtattg
     4561 tccgtgttcc gtaaggcaga aattaacgaa gagcaactgg ctgcagctcc ggtaatcatc
     4621 cgtgaagatc gtgaagcgca actggcagct cgcctgctgc agtttgaaga aaccctcacc
     4681 gtggttgccc gtgaaggcac gccgcatgtg atgtgtgctt acctgtacga tctggctggt
     4741 ctgttctctg gcttctacga gcactgcccg atcctcagcg cagaaaacga agaagtgcgt
     4801 aacagccgtc tgaaactggc acaactgaca gcgaagacgc taaagctggg tctggatacg
     4861 ctgggtattg agacagtaga gcgtatgtaa tcgatttttc gtgagagtaa agcctgatca
     4921 gggttacctg atcaggcttt tttattgcca tctaaatgta ttctgaaaat ggacatgcca
     4981 ttgttttctg actgttggat aagaggccag aagcgtaata tccggcccca gggaaacgat
     5041 aacggttgaa tttaaggaat accgcagtgt ttaaatttct tgtattaaca ttaggcatta
     5101 tctcttgcca ggcttacgca gaagatacgg ttatagaaaa cgaccatgac atttcagcca
     5161 tcaaagattg ttggcaaaaa aattcagatg atgatactga cattaacgtg atcaaatcat
     5221 gcctgcgaca agaatacaat ctcgtcgatg cgcaattaaa taaagcctat ggtgaagctt
     5281 atcgttatat agaacaagtg ccacgcacag gtgtaaaaaa acctgatacc gaacaactta
     5341 acttgcttaa aaaatcacag cgagcctggc tggattttag ggacaaagaa tgtgaattaa
     5401 tcctttcaaa tgaggacgtt caggatttaa tgtacatcgt tccacgtcgc ttcgacaacg
     5461 gtattattct ctgccatttt taccggtttg ggttcggcag gcgctttttg ttcgcgtccg
     5521 cgtatccacc aggccagccc gagcaatccg gcagtgaaca gcaaaaatac caggttcggc
     5581 attccaggca ccaggccgag taaaccgagc acggcggcgc ttaacaacat aacgcttggg
     5641 ttactgaaaa gctgattcac catctgctcg ccaacatcct gatcggtgct gacacgcgta
     5701 acgatgaccc ccgcggcggt aaaaatcacc agcgccggaa tttgtgccac cagaccatcg
     5761 ccaatggtca atagcgtata actttccgcc gcgtgtccca tgctcatgcc atgttgcagc
     5821 acgccgacca gcaacccgcc gacaacgtta atgaccatga tgaggatccc ggcgatggca
     5881 tcgccgcgaa caaacttact tgccccgtcc attgagccgt aaaaatcggc ttcctgagtc
     5941 acttcggagc ggcgtttttt cgcctcatct tcaccaatca atccagcgtt aaggtcggcg
     6001 tcaatcgcca tctgcttacc cggcatacca tcgagaacaa agcgcgcacc cacttctgcg
     6061 atacgcccgg cacctttggt aatgaccata aagttgatga tcacgagaat gacaaacacc
     6121 acgataccga tagcgaaatt gccaccaacg aggaagtgac cgaacgcttc gaccaccttc
     6181 cctgccgccg ccgcgccggt atgcccttcc atcaaaatga tacgggttga agccacgtta
     6241 agtgccagac gcaacaacgt agtaaacaac agaatggtcg gaaacgcagc aaactcaagc
     6301 gtgcgctggg taaacatcgc caccagcaac accatgatcg acaaggcaat attgaaggta
     6361 aacaacaggt cgagtatgaa tgcgggcagt ggcagcacca tcatcgacaa gatcaacagg
     6421 atcaaaatcg gtccggcaag gatctgccat tgtgtcgatt tcaggtttgc gggcaggcgc
     6481 agcatcgcgg ccagattact catgggtcgg tttctcgtta ataaaatcca gggcttccgg
     6541 caccggaaga tgagtaggtt gtacagggcg ctgtccacca gccagacgcc agcgtttcag
     6601 ttgccagacc caggccagca cttccgccac cgcggcgtac agttgacccg ggatttgttg
     6661 accaatctcc gcatgtcgat acagcgctcg cgccagcggc ggcgcttcaa gcgtcgggac
     6721 gttattttca gcgccaattt cacgaatgcg cagcgcgacc agccctgcac ctttagcgac
     6781 cactttcggt gcgctcattt tgttttcgtc atactgcaac gctaccgaat agtgggtcgg
     6841 gttattgaca atgacatcag ctttcggcac atcggccatc atccgacgcc gtgcagcagc
     6901 tcgctgcatc tgacggatcc gccctttaac atgagggtca ccttcgcttt gtttgaactc
     6961 atcacgaata tcctgccgcg acatacgcag ctttttcagg tggctgaaga tttggaaaaa
     7021 gacgtcaaat cccaccattg gaatgacacc aagcaccacc agcagtgcgc atagcccaac
     7081 cagatccatc gcattaccca tggcggtagt cggagactcg gccattaagc gcatcatctg
     7141 cggccagtga tgccagagat aaaaccccgt cacgctgcca accaggatgg ttttcaaaat
     7201 tgccttaagc aact
//
LOCUS       DAJVRF010000174         7210 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJVRF010000174 DAJVRF010000000
VERSION     DAJVRF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.174"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..1214)
                     /locus_tag="OQN13_003569"
     CDS             complement(<1..1214)
                     /locus_tag="OQN13_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCS1616833.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPEWLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKN"
     gene            complement(1355..2695)
                     /gene="gntU"
                     /locus_tag="OQN13_003570"
     CDS             complement(1355..2695)
                     /gene="gntU"
                     /locus_tag="OQN13_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS1616834.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDINEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(2699..3226)
                     /gene="gntK"
                     /locus_tag="OQN13_003571"
     CDS             complement(2699..3226)
                     /gene="gntK"
                     /locus_tag="OQN13_003571"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCS1616835.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3365..4360)
                     /gene="gntR"
                     /locus_tag="OQN13_003572"
     CDS             complement(3365..4360)
                     /gene="gntR"
                     /locus_tag="OQN13_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCS1616836.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(4584..5279)
                     /gene="yhhW"
                     /locus_tag="OQN13_003573"
     CDS             complement(4584..5279)
                     /gene="yhhW"
                     /locus_tag="OQN13_003573"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCS1616837.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5402..6439)
                     /locus_tag="OQN13_003574"
     CDS             complement(5402..6439)
                     /locus_tag="OQN13_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1616838.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYILNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            6772..>7210
                     /gene="yhhY"
                     /locus_tag="OQN13_003575"
     CDS             6772..>7210
                     /gene="yhhY"
                     /locus_tag="OQN13_003575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1616839.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYA"
BASE COUNT         1839 a         1949 c         1742 g         1680 t
ORIGIN      
        1 tcattttttc tctgattcat caaatggtta attcgttcat ggtcaggaga gaaactgacg
       61 atagttctgc gccataattt ctcggtatac accacaggcg atggattttc agctaccaat
      121 aatttcagtg ctttttctat ttgcccataa cggagattcg tccgttgttc aatgcctcgt
      181 agggtaagac cgtcattctc gctaagtacg ttgagtattt catgaatttg cgcctccgca
      241 ggaaaggcgc tttcacggaa gaatttatga atagcgcggt cttcaccacc acaaagcaat
      301 atgccaactg cggaatctat agcacgcccg gcacgcccta cctgttggta gtaaccgaca
      361 atagaccccg gcatttgata atgaatgacg aaacctaaat caggtttatc aaaccccata
      421 cctaatgccg tcgtcgcgac cagcactttg attttatttg ccagcagcgc ctgctccaga
      481 tattccctgg cagtattaga atcttccgcg ccttcacaag tcacgccgct gtagtaagca
      541 aatgcactga taccatttgt ccttaaccac tcggcgacaa gttcggcatc gcgagtagtc
      601 agggtataaa caattcccga tttggaaaac tgagggataa ccgttgctaa ccatgccaga
      661 cgggatgact gttcacctaa gactaaggca tcaagcgcca aactttcgcg agccagcgtt
      721 ccacgctgaa tcacaatgtc acccaattgc tgacggatat cctcaacgac acgattattc
      781 gctgtcgcgg ttgtacccag aacaggggta ttcgcagttt caagaaaatc ctgattcgcc
      841 aaccattcag gtgaaatcag cagacaatct acaccacccc gcagcaactt atcgctaatg
      901 cgctgccatt cttccctgtt tgtagaattc aacgtttcag ccgtaatacc taaccgttca
      961 gcggcggcaa cctggttacg catcagggca agcaaaggag agatgataat tgtcgggcca
     1021 gcgccgcgat cgcggaagat tttactggcg atgaagtaca cggcactttt cccccagcca
     1081 gtgcgttgga caaccagcag ttttcgccgc tgattaacca ccgcatctat agcttcccat
     1141 tgcccttccc tgaaagtagc gctggtgtta cttaacatcc gctgtaacag caattcagct
     1201 ccgtgtttct ccatcatctt ctccctcgtc cgttaacgca gtgattagaa cggataatgt
     1261 tccggtcttt ttgcgagaga gatcaaaaag atgaagacag gagataagag aaatgaatta
     1321 ctgcgagcaa gctcgcagta atttgtcgca gtaattaact caacagctgg aacgcaatca
     1381 tcccaacaat ggcaccaaca gtgccgagga tggtttccat catggtccag gttttcagcg
     1441 tttcggcttc ggtcgcgccg gtaaatttac cgaacaacca gaaaccagcg tcgttaacgt
     1501 ggctgacaac aatcgaacca ccggcgatac aaatcgacag cgccgccatt tgcgcaccgg
     1561 aattgttcag ttgttcaatg accggcatca ccagccccac cgccgttaaa caggcaacgg
     1621 tggcagaccc ctgaatgatg cgcactgcag ctgccagcac gaagcaggtg atagcaatcg
     1681 gcaggcccat gccggttaac gcttcgccca gtgccggacc tacgccagag tcaaccagca
     1741 cctgtttgaa cacgccgcct gcaccaatca ccagcagaat gatccccgcc ggttgcagcg
     1801 cgtgaccgca aatctccatc actttgtctt ttggcatgcc ctgacgcatt gccaggccat
     1861 aaatcgctac cagacaagca accagaatcg cggtaaacgg atgaccaata aactcgaacc
     1921 attcgtaagc ggtagagcct tctggcacaa aacgcgcggc aatggttttc agccccacca
     1981 gcaccagcgg caacaggatc agcgacaggc tgaatccgaa agatggcatt ttaccttcgc
     2041 cgagatgcgg ctcgttgatg tcgtcaggaa tatgcagctc aacgtaacgg ctgatgaagt
     2101 taccccacag cggcccggca ataatcattc ccggaattgc cgcacacagg ccaatcagga
     2161 tcatccagcc aaaatcggcg ttcatctgcg atgccagcag cattggcgct ggccctggca
     2221 ccaggaacgc cgccgctgcc gccacgcctg cgaataatgg gattatcagc ttcaccaggt
     2281 tcgtatcggt gtggcgcgcc attgagaaag caacgctaat cagcagaaca atcgccactt
     2341 caaagaacag cggtagcgca cagaccagcc ccgcaaggcc gatggcataa tgcgcgcggc
     2401 tgtgaccgaa ggatttgagc attttgacgg caatctgatc gactgcgccg gtttcatgta
     2461 agatcttgcc aaacatagct cccagggcga caaccaccgc caggaagccg agggtgcctc
     2521 ccatcccttt ttccatcgtc gctgcgattt tatcgagcgg cataccggaa aaaaggccag
     2581 cccccatgga caccaccatc aaagccagga aagcgtgcat acgcgccttc atgactaaaa
     2641 acagcagcag taaaacagac cctactgctg ttaaaacaag cgttaatgta gtcactactt
     2701 atttgccttt tttaataacc tcaatggtgc ttgccacaac accttccagc ggttgatcga
     2761 tatccaccac cagtacatcg gtttcgtccg cacccggctc ctgcagcgtt tcaaactgcg
     2821 tcaccaacat ttgggtttta aagaaatggc ctttgcgcgc tttcaggcgg ctttcaatca
     2881 cgtcaaaatc gcctttcagg tagatgaaag agagattcgg attaccttca cgcagcaagt
     2941 cgcgatagtg ttttttcaat gcagaacaga cgatcagtga cactttatta gtgcgctgca
     3001 tagcaaacgc ggcgtcgttc agcgcctgca accagggttt gcgatcgtcg tcattcagcg
     3061 gttcgccaga cgccattttt tcgatattgc gccgtggatg gaggaaatcg ccatcaagaa
     3121 acgcggcatg aagttgatgc gccacttcac tggcgaccgc agatttgccg ctgcccgata
     3181 cgcccatcaa gacgtaaatg tggtgatcat ggttagtcgt gctcaaagtg gtgcccccac
     3241 aatacaagaa ttaaaaatgt tacgggtaac tgttatcggt aacattgtcc agccggacaa
     3301 tatcagaagc aatatccatc cgtgccttaa gtgtataagt gtgagctact tcaaatttgt
     3361 aggcttaaat agatccgccc ggtgacaagg tgaaacctaa atctaacatt ttcggtgtca
     3421 cagattcgcc acgaatacgc gccagcaggc gttcagcgcc aatactgccc atccgctcac
     3481 gcggcgtcag cacgctcgct aaccgcggct ccatcacctg gccaatgtca tgaccgtgga
     3541 aaccggcaat cgccatatcg tcaggaacct tcaatcccag acgctgacat tcaaacgccg
     3601 cgccgaccgc caggtcatcg ttggtacaga acacgccatc cagctgcgga tattcccgcc
     3661 gcgcctggcg aatcagttca ataccggaag agtaagaaga agattgctca accatcacgc
     3721 tatacggcac cagacctgca tccagcatcg cctgttcgta tcccttctgt ttgatgatag
     3781 tacgttcgtc gagacgcgcg ccgagatagg caatgtggca atgcccgcga gcaataatgg
     3841 cggtggtcat ctggcgtgcc gcttcaaagt tatcaaaacc gacggcgata tcgaggcacg
     3901 gcgactggct gtccatgagt tccaccaccg ggatccccgc cacttcaatc atctttaagg
     3961 tgcgcggcgt gtgggtacgt tcggtgagga tcagtccgtc gatattccag gagagcatgg
     4021 attcgagacg ttcttgctcc atttccggtt tataaccgta gtgagccagc atggtctgat
     4081 aaccgtgcgc gtcggtgacg ctttcgattc cgcgtaatac ttccgcgaaa acctggttgg
     4141 tgagagaagg taacaggacg ccaatcgccc ggctggtggc gttagagagg atatcgggcg
     4201 cacgattggg aatatagccc agttcatcaa gagcggcggc aatcttgccg cgtagagcga
     4261 cggaaacctg ctccgggttg cgtaaaaaac ggctgaccgt cattttggtc acgcctacac
     4321 ggtcagccac atcctgaagt acgggtcttt tctttttcat cgtcctgaag gtacaaaaga
     4381 gatagattct cttagtttaa cacggacgca tcagaacctt cccggggaaa acagtgcttg
     4441 tttacaatta ttagatatac actaaatcaa ttgcatcgca ttgtgctaac gacgcggtaa
     4501 aaccccggaa gcgtgcatca gcacgcgacc ggagcgagcg aggaagcctg caaaaaggca
     4561 ggcttcaaag atgccgttga gttttaaacc ggcggcagat cgaacagtaa cacttcgctg
     4621 tcgctatccg catggatgga gattgcctgc tcatcccaga ttgccagacc atcgctggtc
     4681 gaggctttca cgccgttaat ggtgacattg cctttcacca cctggatcca gacgcggcgt
     4741 tcagcggcaa tctgatgcac cgactgctca tctttcagca acgcccagcg gtacagttcc
     4801 atatcctgat gcactttcaa tgaaccatct cgcgcatccg gcgagagcac cagctgtttg
     4861 ccctgtacgg catcgaagcg acgctgttca taacgcggcg taataccgtt ttcttcaggc
     4921 atgatccaga tctgatacag atgcagacgc tcggtgctgc ttgggttgta ctctgagtga
     4981 cgaatacccg tacccgcact cataatctgg aactcacccg ccggaacctg ctctttattg
     5041 cccatgctgt cctgatgctc aacagtacct tccagcacgt aggtcaaaat ttccatatct
     5101 ttatgcgggt gagtgccgaa gccctgccct gcttcaatca cgtcgtcgtt aatcacgcgc
     5161 agcgcggaga agcccataaa gttcggatcg taatagttgg caaaagagaa agtatgccag
     5221 gagtccagcc agccatgatt tgcatgacca cgttcatttg ctttgcgtaa gtagatcatt
     5281 tgtattcgcc ccatgaatga tttcgatggg cttagtgtgg acccgatctg cctgggatga
     5341 tagagggtga aaattgaccc ctctgttcaa aaaaattgaa ctattcgagg ggccatcaaa
     5401 cttacttcgc gagagtcact gtggagggag aggcttgctc aaatccgcgt tcaaggattt
     5461 ccagattggt aagaacttca gattccttga cgtaatttgg cgcaccgtgg gtgatggttt
     5521 gatacaacgc atcataaacg cgcccgtaat cgcccatctc cggcttcatc tcttctctga
     5581 ccgtcacgcc ctcgtcattg acatactcca gcacaccgac cgaatcatcc gctgcgaatc
     5641 ccggttcgcc cggcataata ttagccttca ggctggtttc ctgctggtcg ataccgtatt
     5701 taataaacga acctttctta ccgtgaacga taaatttcgg ataatcgatt ttcaccagat
     5761 ggctggtttt gacgatggct ttcaggtcgc cataaaacag ttgcgcttca aaggtgtcgt
     5821 caggattggc tttattacgc aggctgcaga tgtcataagc gacgtgatcc gggcgaccga
     5881 acagagaaat aatctggtcc atcgtatgca caccaaggcc atagaacgcg ccatcctgcg
     5941 gcaacccagg tttggtttct gccaccgggc ggtaatagtc aaaatggctt tccacttcaa
     6001 caatctctcc cagcttaccg ctttcaatcg cttttttcgc cgtcaggaag caggaatcaa
     6061 agcgacgatt ctgatatggc gtgacggtca gccctttgct tttcgccagt gcaaacagct
     6121 ctttcgcctg cgcaagtgtc ggagtgaacg gtttttcgac cagcacattt ttcccggctt
     6181 ccagcgcgcg cttcgcgtac tcgaagtggc tgtccgcatg ggtgcagaca acaaccaact
     6241 taacatcggg atcgtttagc acttcgtcga gatcgctggt gaaatggata tgagagtaaa
     6301 tgggagcctg ttcttccggc ttcgcatggc gacgaaaaat atgcgcgaca tgccagctat
     6361 ccttgcggtt aagtatatac ggcagatggt aacgggtggt gcttttgccg aagccaataa
     6421 aggcgcagtt gatgaccatg atttcgtcct ttttaaggtg gttatttaca ccttagcgca
     6481 aagcggacgt ggttcctact ggagtgcgca taacgaacac aagcactccc gtggataaat
     6541 tgagaacgaa agatcaaaaa aaagccagca cccggctggc taagtaatac tggaagcaat
     6601 gtgagcaatg tcgtgctttc aggttctccg cgagggtctt cctgatcgcg agacaataat
     6661 aatcattctc attcgcactt gtccaacact ttttgcaaaa aaaatgcatt tgactcgcat
     6721 ttgaaagtca atgatgttga aagggacatt taccccaaag aggacaaagg aatgagtgag
     6781 atagtaatac gccacgcaga aacacgggat tacgaggcca tcaggcagat tcacgcccag
     6841 ccggaggtgt attgcaacac actacaggtg cctcatcctt ccgatcatat gtggcaggag
     6901 cgactcgccg atcgtcccgg catcaagcaa ctcgtcgcct gtattgatgg agacgtcgtg
     6961 ggccatctca ccattgacgt gcaacagcac ccacgccgca gtcatgttgc cgattttggt
     7021 atctgtgtcg actctcgttg gaagaaccgc ggcgtcgcca gcgccctgat gcgagagatg
     7081 attgaaatgt gcgacaactg gttgcgggta gatcgcattg aactaaccgt gtttgtcgat
     7141 aacgccccgg caattaaggt ctataaaaaa tacggctttg aaattgaagg gactggtaag
     7201 aagtacgcat
//
LOCUS       DAJVRF010000175         7157 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJVRF010000175 DAJVRF010000000
VERSION     DAJVRF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.175"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..3311)
                     /gene="hrpA"
                     /locus_tag="OQN13_003576"
     CDS             complement(<1..3311)
                     /gene="hrpA"
                     /locus_tag="OQN13_003576"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCS1616840.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYG"
     gene            3512..4117
                     /gene="azoR"
                     /locus_tag="OQN13_003577"
     CDS             3512..4117
                     /gene="azoR"
                     /locus_tag="OQN13_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCS1616841.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(4168..5484)
                     /locus_tag="OQN13_003578"
     CDS             complement(4168..5484)
                     /locus_tag="OQN13_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HCS1616842.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(5474..>7157)
                     /locus_tag="OQN13_003579"
     CDS             complement(5474..>7157)
                     /locus_tag="OQN13_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCS1616843.1"
                     /translation="PALQPGAKKVIVLFHRGHEHSGRLQHIVDELAMPDTAFYAWDAR
                     GHGKTSGPRGYSPSLARSVQDVDEFVRFAASDSQVGLEEVVVIAQSVGAVLVATWVHD
                     YAPAIRGLVLASPAFKVKLYVPLARPALTLWHRLRGLFFINSYVKRRYLTHDRQRVAS
                     FNNDPLITRAIAVNILLDLYKTSERIIRDAAAITLPTQLLISGDDYVVHRQPQIDFYQ
                     RLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQSFIRRLYANKSQKFDYQHEDRTGPS
                     ADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGTHSAGLHLGMSTGFDSGSSLDYVYQN
                     QPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQILIKQAVADLHVKGLAVRVVDIAAG
                     HGRYVLDALANEPAVSDILLRDYSELNVAQGQEMIAQRGMSGRVRFEQGDAFNPEELS
                     ALTPRPTLAIVSGLYELFPENEQVKNSLAGLANAIEPGGILIYTGQPWHPQLELIAGV
                     LTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCTQRIDEWGIFTVSMAVRRDN"
BASE COUNT         1754 a         1981 c         1741 g         1681 t
ORIGIN      
        1 ttgccatacg ggttaaagta cagccccagc ttggctttgt ttggtaactt ctcatgtaaa
       61 tacttgatcg gcgagggaat attcagcagc agtagacggc gaagaccgtt ccacattacc
      121 tgtttttgct ccagcggatt atcaaacagt ttgatcgcca cactgtcgcg ttcatccact
      181 agcgctggcc acgccttcac tttgtagttg ccacgcttct gttcgtagct ttccggcagc
      241 tgaccaaaac tccagatatg cagtccgctc tgctcgatac cgtcatccgc caccgcagac
      301 agcgtttcct gcactttgcc tttcagcgca tctttcagat cttgtagcga gcgcccttct
      361 tttagcttct tgtttttgtc atccaccacg cggaaggtga ttttcaggtg atcgggcacc
      421 tgatcccagt gccagtcttc gcggtcaacg gtaacgccgg tcatccgccg taactcgcgc
      481 tcaaggctgt cgagcaacgg taactccagc ggtgtgacgc ggcctaaaaa cgcttcggcg
      541 tagtttggtg caggcacaaa attacggcgt accggtttcg gcaacgattt aatcagagca
      601 atcaccaatt cgcggcgcag accggggatc tgccattcaa acccgctttc ctcaacctgg
      661 ttaagtaacg gcagcggaat atgtacggtc acaccgtccg cgtccgcgcc tggctcaaac
      721 tgatagctca aacgcagctt gagattgccc tgatgccaga agttcgggta atccagcttt
      781 ctgatttttt ccgccccttc tttgatcaac atgctctttt caaagttgag caaatcaggc
      841 gtttcgcggc tgactttttt ccaccagcta tcgaagtgac gagcggagat tacatcgtgg
      901 ctgatgcgct ggtcgtagaa ctcaaacagc gtttcgtcat caaccagaat atcgcggcga
      961 cgtgatttgt gttccagctc ttccacttcg gcccgcagtt tcaggttttc acggaagaat
     1021 gcgtgacgcg tctgccagtc accttccacc agagcgtggc gaataaagag ttcacgacat
     1081 aacgccgaat cgatctggct gtagttgacc ttgcgcgcag caacaatcgg caaaccataa
     1141 acagtgactt tttccgttgc catcaccgcg ccctgcgccc gttcccagtg cggttcgctg
     1201 taggtgcgtt taatcaaatg ctgagcaacg ggttctaccc attccgggtc gatacgcgca
     1261 gcaatgcgcc cccacaggcg gctggtttct accagttccg ccaccattac ccatttcggc
     1321 ggctttttga ataaaccaga accggggaag atggagaaac gcgcgttacg tgcaccggta
     1381 tattcctgtt tatcggcatc tttcatgccg atatgggaaa gcaaaccggt cagtaaggcg
     1441 atgtgaattt cacgatactc cgccggttcg ctgttgaccg gaatgccaag ttctttcacc
     1501 acctggcgca actgggtgta gatatcctgc cattcgcgca cgcgcagata gttgaggtaa
     1561 tcggtacgac acaggcgacg gaaggcgttg gaagaaagcg ccttttgctg ctcgccaaga
     1621 taattccaca gatttacaaa cgcgaggaag tcggattctt tgtcgtggaa gcgacgatgt
     1681 ttttcgtccg atgcctgctg tttgtccatc ggacgttcgc gcggatcctg aatggagagc
     1741 gcggacgtga taatcatcgc ctcacgcacg cagccatgtt tttgcgcttc cagcaccata
     1801 cgcgccagac gtgggtcgac aggcaactgc gagagctggc gaccgagcgg cgtcagttta
     1861 taggcgctgg cctgttcatc cgtggtgatc gcgcccagtt cttcgagcag acgcacgcca
     1921 tcctggatat tgcgtttatc cggtgcttcg acaaacggga acgcagcgat atcgcccagc
     1981 cccagcgcgg tcatctgcaa aataaccgag gccaggttgg tacgcagaat ctccggatcg
     2041 gtaaactccg ggcgcgagag gaaatcgtct tcggaataga gacgaataca gatcccttcg
     2101 gacacacgac cacagcggcc tttacgctga ttggcagacg cctgcgaaat gggttcaatc
     2161 ggcaaacgct gcactttggt gcgatagctg tagcggctga tacgcgctgt accggggtcg
     2221 atgacgtatt taatccccgg tacggtcagc gaggtttccg cgacgttggt cgccagcaca
     2281 atgcgccgtc cgctgtgcga ctggaacacg cggttctgtt cgctgttcga aagccgcgca
     2341 taaagcggca agatttcggt atggcgtaag ttcagcttgt tcagcgcatc ggcggtatcg
     2401 cgaatttccc gctcaccact cataaagatc agaatgtcgc cagggctttc ctgactcagt
     2461 tcgtctacgg cgtcaaaaat cgcctgcaac tgatcgcgct cggtgtcatc ggcttcttca
     2521 acaatcggtc gatagcgcac ttccaccgga taggtccgac cggagacttc gataatcggc
     2581 gcattattaa agtggcgcga aaagcgttcc gggtcgatag tcgcggaagt gataatgatt
     2641 tttaggtcag gacgccgcgg cagcaactct ttcaaatagc cgagcagaaa atcgatattc
     2701 aggctgcgtt cgtgcgcttc gtcaataatg atggtgtcgt actgcatcag caggcggtct
     2761 tgctggatct ccgccagcag gataccgtcg gtcatcagct tgaccatcgt gttatcactt
     2821 acgtgatcgc tgaaacgcac tttgtaaccg atgcaaccgc ccggctccgt tttcagctct
     2881 tccgcaatac ggttcgccac cgttcttgcc gccaggcgac gcggctgggt atggccgatc
     2941 agccctttaa tcccgcgccc cagctccata cagattttcg gtaactgagt cgttttacca
     3001 gaacccgttt ccccggcgac gatcaccacc tggtgatcac gaatcgcttc gagaatgtcc
     3061 tgttttttct gactaaccgg taaattgtca ggataagtaa tttccggtcg tgccgcttca
     3121 cgcagcagga ctttccctgc cgcctggtca atctctttcg ccatctcctg gaaaatggcc
     3181 tgttgtgcat caggattttt aaccttcttc acgccgtgca gacggcgaga aaaacgcagt
     3241 ctgtcacgca gcatcagcga atccagccgc tgctgcaagg ccgtaaaggt caatttttgt
     3301 tgttctgtca taacgttagt gggcagtagt ctgcctgatt aattttcttt gaaatgatag
     3361 atatagagta ccacatcacg gctttctatg cgttgttcaa taaattcgaa cataggcttc
     3421 gatttattgc gctatctctg tggcaggatt gtgaataaag tgtcaacaag caacggggca
     3481 tcgcccattc aaacatctat aaggaaacac catgagcaag gtattagttc ttaaatccag
     3541 catcctggca gggtactctc agtctaatca gttgtccgat tattttgttg aacaatggcg
     3601 cgaaaagcac tccgctgatg aaatcaccgt tcgcgacctg gctgcaaatc cgattccggt
     3661 actggatggc gaactggttg gtgcactgcg tccgagcgat gcgccgctga ctccgcgtca
     3721 gcaggaagct ctggcgcttt ccgatgagct gattgccgag ctgaaagccc acgacgttat
     3781 cgttattgcg gcaccgatgt ataacttcaa catctcaact cagttgaaaa actattttga
     3841 cctggttgca cgcgcaggcg ttactttccg ctataccgag aacggtccgg aaggtctggt
     3901 aacgggtaaa aaagccatcg ttattaccag ccgcggtggg atccacaaag atggaccaac
     3961 ggacctggtg acgccgtatc tgtccacgtt cctcggcttt atcggcatta ctgatgtgaa
     4021 atttgtcttc gccgaaggga tcgcatacgg tccggaaatg gcagcgaaag cgcagtctga
     4081 cgcgaaagca gccatcgaca gcattgttgc tgcataaccg gacacgatct tgatgtggac
     4141 tgtagtccag tccacatccc aatccactta cctgttttcc cataatttca gcatcgcttt
     4201 gtgatcttcc ttaagcacaa tatgcgagcg tctggctcga ataaagctaa tcgcttcatc
     4261 aacggtttta cagtatccgt aacataacag ccatgccgcc actaccagcg cactgcgcga
     4321 taatcccagt gcgcaatgga ccagaacgct gccttgctct tcgcgtaatg tttccagcat
     4381 cgccacggcc tgtcggagct ccccctcttc cggaaccacc agatctagca tcggtacaca
     4441 gaaatagagt cgatcttttg tcgctcgtcc acgagggaat tcaaaggtga cgtccagaac
     4501 cgcattctgt gccggaatat gtcgtggaaa cgcccctaaa taaacaccag cagtcatttt
     4561 gctcaccggc tccaggcgac gacaaaacca acgcatagat agccacatcc cgatgcgcca
     4621 gggcaatgtc agccagtaaa cggcgggcgg gagtttcccc tgactatctt tccctgttgt
     4681 tatcgcgcca agcccaccgt acccacggcc aataatgagt agcgataata ctggccaaca
     4741 taaccagact gaccaccata actgaaccat catcattagc tccatcaaca caatgcacgc
     4801 gcacgcgcct acgacatatg gcagtgctat tttgattcga cgttgatcag gtttctgata
     4861 attccaacga cggtcgacgg gcaccatcca gtcaatcaac atacctaccg ccagccctgt
     4921 gatgacatca ataaaatgat gctgccatgt cgtcagcgtc gaaatggcga tgagtaaaaa
     4981 ccatccgccg cagactttac gccacctcac agccagatgc tgacgaaagt gacgccaaag
     5041 tagccagcag agaataatat gcagcgaagg agactggtta taaggcagat caaacagttc
     5101 aagttgcgaa aatagccatc ccgtcacccc actcacttca ggacggataa aactaaactt
     5161 cagcggatag agcaaaaaac cgcagcaggc cattaccgtt gccagaataa gccggtggac
     5221 aagtcggcgc tgttcgaatg tcgtgctaca aacgaacagc gaaaatccat ataaaagatc
     5281 cagactccag taaggaacaa tagtccaggg aagaaaaggg atcgccgttt cccaaccgaa
     5341 tacctgactg ggaatatcat ggctattact gtcctgaacc gcggtgaact gattaagaga
     5401 tccataggtg aagaagaaaa acggggccag caacaataac cagccagctc cttgtagcaa
     5461 tacgttccgg cgttcagtta tcacgacgca ccgccatcga aaccgtaaaa atgccccact
     5521 catcaatccg ttgtgtgcat ttatcaaatc cagcatcatg cacgagcaaa tccatctccc
     5581 cttgcgtacg cacgcgcatt acccacagtt taccatcttt atgactggtt aacaccccgg
     5641 caatcagctc cagttgtggg tgccacggct gcccggtgta gatgagaatg ccacccggtt
     5701 cgatggcatt ggcaagacct gcgagtgagt tttttacctg ctcattttcg ggaaacagct
     5761 catacagtcc agagacaatc gccagcgtag gccgcggagt taacgcgctg agttcctccg
     5821 ggttaaacgc atcgccctgt tcaaaacgca cccgcccaga cattccccgt tgggcaatca
     5881 tctcttgccc ctgtgcaaca tttaactcac tgtaatcacg taataaaata tcgcttacgg
     5941 caggctcgtt tgccagtgca tccagtacat agcgcccatg ccctgcggca atgtcaacca
     6001 cgcggacggc taaacctttg acgtggagat cggcaacggc ttgtttaatc agtatttgta
     6061 aatgagtttt gcgctggcga ataccgcgcc agccaacact gttcaggtaa attttgtcga
     6121 ttaagcgccc gaatgcgtta ctgccttgcg gttgattttg atagacataa tccagcgaac
     6181 tgcctgaatc aaagccagtg ctcattccga gatgcaggcc cgcagagtgc gtcccgaaca
     6241 atttcatcgc tttacgcata aagcgatacg ccaaatcaac cggcgataat ggcacgggtc
     6301 cacctgaaag gaggcgccag cgatccgctg atggtccagt gcggtcttca tgctgataat
     6361 caaatttttg tgacttgtta gcatataaac gacgaataaa gctttgcatt ttttcaaatg
     6421 cctgcgccct gttctcttca cccaacgtgt cgtgataaaa gcctggcagc agatgcagct
     6481 ctttcagagg gctacgtaat ctctgataaa aatcaatctg cggttggcga tgcaccacat
     6541 agtcatcgcc tgatatcaga agttgcgtgg ggagcgtaat cgccgccgca tctctaataa
     6601 tacgttcaga cgttttatag agatcgagca agatattgac ggcaatcgcc cgtgtgatca
     6661 gcggatcatt attgaaactc gccacccgtt gccgatcgtg ggtcaaatag cgtcttttca
     6721 cataggaatt aataaaaaac agaccacgca gacgatgcca tagcgtcagc gcaggacgtg
     6781 ccagcggcac atacaattta accttaaagg ccggagaagc cagcaccagc ccgcgaattg
     6841 caggcgcata atcatgaacc catgtggcaa ccagcactgc gccgacgctt tgcgcgatca
     6901 caaccacctc ttccagtcca acctggctgt cgctggcagc aaaacggaca aattcatcga
     6961 catcctgcac tgaacgcgca agagatgggc tataaccacg cggccccgaa gtttttccat
     7021 gcccccgggc atcccatgca taaaaagcag tatctggcat cgccagttca tcaacgatat
     7081 gttgtagacg accagaatgt tcatggccac gatgaaataa gacgatgacc tttttcgccc
     7141 ctggctgtaa agcgggc
//
LOCUS       DAJVRF010000176         7122 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJVRF010000176 DAJVRF010000000
VERSION     DAJVRF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.176"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            5..436
                     /locus_tag="OQN13_003580"
     CDS             5..436
                     /locus_tag="OQN13_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCS1616844.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQFA
                     VQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            519..1421
                     /locus_tag="OQN13_003581"
     CDS             519..1421
                     /locus_tag="OQN13_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCS1616845.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1476..3638)
                     /gene="uvrD"
                     /locus_tag="OQN13_003582"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1476..3638)
                     /gene="uvrD"
                     /locus_tag="OQN13_003582"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCS1616846.1"
                     /translation="MDVSYLLDSLNDKQRETVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3722..4438)
                     /gene="yigB"
                     /locus_tag="OQN13_003583"
     CDS             complement(3722..4438)
                     /gene="yigB"
                     /locus_tag="OQN13_003583"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCS1616847.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4438..5334)
                     /gene="xerC"
                     /locus_tag="OQN13_003584"
     CDS             complement(4438..5334)
                     /gene="xerC"
                     /locus_tag="OQN13_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCS1616848.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5331..6038)
                     /locus_tag="OQN13_003585"
     CDS             complement(5331..6038)
                     /locus_tag="OQN13_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCS1616849.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6035..6859)
                     /gene="dapF"
                     /locus_tag="OQN13_003586"
     CDS             complement(6035..6859)
                     /gene="dapF"
                     /locus_tag="OQN13_003586"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCS1616850.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6896..7099)
                     /locus_tag="OQN13_003587"
     CDS             complement(6896..7099)
                     /locus_tag="OQN13_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1616851.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1640 a         1990 c         1854 g         1638 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcgacat cgttcgtctg gatgccttca aaaccagtaa
      121 agagattcag tttgcggtgc agtcagggcc aatgttgatg gaaaacagtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt gatggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagaggaaa ggttaagcat tgtacactcg aggaggccag acaaactggg gctggctaca
      481 cagctgaaaa atcatcagat aaatgaaggg aaacaaaaat gaaaaaaacg ctcgcaacgt
      541 tatttttatt aacgtgtctg ggatcatcat ccgcttatgc ggataacgct ttgattttac
      601 aaactgattt tagcctgaaa gatggtgccg tctcggcgat gaaaggggtc gcattcggtg
      661 ttgaccataa tcttaaaata tttgatttaa cacacgaaat tccaccgtat aacatctggg
      721 aaggcgctta ccgcctgtat cagaccgcca gttactggcc gcaaggttcg gtatttgtca
      781 gtgtggtcga tcctggtgtt ggtacagacc gtaaatcggt agtgctaaaa accaaaaacg
      841 gtcaatactt cgtttcacca gataacggta cgctcacttt ggtggcggaa tcgctgggta
      901 ttgagagcgt ccggcaaatc gatgaaaaaa ccaaccgcct gaaaggttcc gaaaagtcat
      961 ataccttcca cggtcgtgac gtgtatgcct acaccggtgc acgcctggct tccggagcga
     1021 tcacgttcga acaggtgggg ccagagcttc ctgcaaaagt tgtcgaactt tcttatcaga
     1081 aagccaaagc aacaaaaggt gaagtgaagg gcaatatccc gattctcgat attcagtacg
     1141 gcaacgtctg gagcaatatc agcgacgagt tgctgaatca ggcaggaatc aaactcaacg
     1201 acacgttgtg tgtaaccatt tccgaaggtt cccagcaaaa atacgcaggg aaaatgccgt
     1261 atgtcgcaag cttcggcgat gtgccggaag gccagccgat ggtgtatcta aacagcttat
     1321 tgaatgtttc cgtggcgctc aatatggata atttcgcgca gaaacatcaa gttgcgtctg
     1381 gtgctgactg gaatattgat gtgaagaagt gcgctaagta aggtgctgat gcaggatgcg
     1441 gtgtggcgca ccgcatcctg caacggagtt gtaaattaca ccgtctccag ccgggcgtaa
     1501 gccgccacca gccatttgat tccctggccc tggaatgcca cctgcaaacg gctatgttca
     1561 ccgctgcctt ccatattgac gatggtgcct tcaccaaact tagcgtggcg cacgcgctgg
     1621 ccgagtttgt agccgctgtc gttctcgacc atcggcgtac ccatccgctg atggctaacc
     1681 gggcggctta ccgtggcgcg caggcgcacc tcttccacac actcttccgg cagctcgccg
     1741 ataaagcgcg acgggcgatg gtaaacctct ttgccataca gacggcgagt ttccgcgtag
     1801 gtcagcgtca gtttctgcat cgcacgggtt acgccaacgt aggccagacg gcgttcttct
     1861 tccagacgtc cgccttcatc cagcgacatc tggcttggga acatgccctc ttccataccg
     1921 acgataaaca cctgcgggaa ctccagccct ttcgccgagt gtagcgtcat caactgcacc
     1981 gcatcctgcc aagtatccgc ctgcccttca cctgcttcca gtgccgcatg ggagaggaac
     2041 gcctgcagcg gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg cgttgccgtc
     2101 accagttcct ctaagttttc gatacgcgtc tgaccttttt cgcccttctc ctgctcgtac
     2161 atggtacgca ggccggagtc tttaattacc cggtcagtct gtacatgcag cggcatatcg
     2221 gcagtttctt gcgctaaggc atcgattaat tccataaatc gctgcaaggc gctggcagct
     2281 cgcccggcga gggctttttc ctgcaacagt tcacgacatg cctgccagag tgttaactgg
     2341 cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt caccaatacc ccgcgttggc
     2401 gtattcacca cgcgctcaaa cgccgcgtcg tcgttacggt tggcaatcag acgcagatac
     2461 gagagcgcgt ctttgatttc ctggcgttcg aagaagcgca tcccgccgta aatacggtac
     2521 ggcatactgg cctgcaacag tgcttcttcc agtacacgcg actgggcgtt actgcggtag
     2581 agaatggcgc actcggcaag cgccccgccg ttgtcctgcc aagttttgat gcggttaacc
     2641 acaaaacgcg cttcgtcgag ttcgttaaaa gcgcaataga gggaaatagg ctcaccgtcc
     2701 gcgccatcgg tccacagttt tttacccaga cgcccgttgt tgttttcaat cagggcgttc
     2761 gcggcgctca gaatattgct ggtagagcgg tagttctgct ccagacggat agtttcagca
     2821 ccggggaaat cattgaggaa acgctgaata ttctctacct gcgccccgcg ccagccgtag
     2881 attgactggt cgtcatcgcc aacgatcatc actttgccgg tatcgcccgc cagcaggcgg
     2941 atccacgcgt actgaatgtt gttggtatcc tggaattcgt ccaccaggat gttggtaaaa
     3001 cgttcgcggt agtgttgcag gatatgcggc ttgttaagcc acaactcgtg agcgcgcagc
     3061 agcagctcgg cgaagtccac caggcccgca cgatcgcacg cttcctgata cgcctgatac
     3121 accttctgcc aggtctgctc caccggatta ccgtagcttt gaatatgatg cggacgcagg
     3181 ccttcatctt tctggctgtt gatgtaccac attgcctggc gcggcggcca ctgcttctcg
     3241 tcgaggttca tggctttgat cagacgctta agcaggcgta gctggtcttc actgtcgagg
     3301 atctggaaat cctgcggtag attggcgtcc atatggtgcg cacgcagcaa acggtgcgcc
     3361 agcccgtgga aggtgccgac ccacatgccg ccctggctgg tgcccatcag ttgcccgata
     3421 cgatgacgca tctccgccgc cgctttgttg gtaaacgtca cagccataat cgagtatggc
     3481 gagcagtttt ccacgctcat cagccaggcg atacgatgca ccagtacgcg cgtcttacca
     3541 ctgcccgcgc ccgccagcac cagaaggttg ctgcgtggcg cggccaccgt ttcgcgctgt
     3601 ttgtcattaa ggctgtcgag caggtaagaa acgtccattg gcaccgccgc gtaaaaatag
     3661 gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg gtatatatac agatttactg
     3721 attatatcag cgaggtcaga gatgccaacc gggaaatttc cagatgcggc agtaaacggc
     3781 tgtcccaggt ttgcatcaga tcgccatttt ccggtctgat ccaacaagcc tgcattccgc
     3841 tgcgaattgc cccacccacg tcagtggtga gatcgtcccc aacatgtaag atctcgccga
     3901 tcggcacgtt gagtttttcc gcagccaaaa agtacatatc gctgaacggt tttgagcgcc
     3961 catgcgggcc agcgcgcagc acaaactcaa aataatcccc caaaccaaac agttccggct
     4021 gggcgttacc gttggtgatc gccaccagcg gccatttctt cgccagctgt tttaaggtgt
     4081 cgtgagtttg ctgcgggacg tcgattcggc tgcgccattt ggcaaagtta atcattgctg
     4141 cgtgtgcgcc tgcactggct tcttcggcac tcagcccggc gtcgagcatc gcttgttcaa
     4201 tcgaacgaaa acgccaacgc gtcacgtcgt gataaatctc gggttccgct tcccgtaccg
     4261 cctggcgcag gcgttgcaga tcttcattct ggaagctgcg cagcgccgga tgataatttt
     4321 gcacaaaggt aagcgcctct cgctcggtgc gcaaaatcac cggacggtta tcgtaaaggg
     4381 tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa aaacgcatta
     4441 tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt gttgaaaatc
     4501 aagatgagta tagatttgcg tggtggagag gttggcatga cccagcagct cctgcacacc
     4561 acgaagatcg ccgctcgact ccagcatatg cgtggcgaac gagtgacgta atttatgcgg
     4621 atgaacgtga ttattcagcc cttgttttat gccccattcg gcaaagcgtt tctgcacatt
     4681 acgcgcggag atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt cgctaccaaa
     4741 caggtcgcgc aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac caatcggcag
     4801 gcggcgctct ttgctgcctt tccccattac ccacacttca ccagactcca ggtcgaggtg
     4861 tttgatatcc agccccacca gctcggaaag acgcagaccc gcgccgtaca tcacttccag
     4921 cattgcacgg tcgcgtacag cgaggggatc gttgatatca atgtccagca gccgattcat
     4981 atcgtcgacg tcgatatttt tcggcagatg acgcggcgct ttcggtgccg aaacaccttt
     5041 cgccgggtta gctttgagtt cgttctggct gaccagccag tcaaaaaagc tacgtagcgc
     5101 agaaagccgt aacgcgaggc ttgctgctcc cagcccttta cggcgactgc gtacagcaaa
     5161 attgcgcacc atcgtcacat cacattgctg ccagctttgc aggccgtttt cgctggcaaa
     5221 attgatgatc gcctcaagct gacgctggta gttaagcagg gttatcgggc taagctggcg
     5281 ctccacgctc agataacgta ggtagcgttc tacatcggtg tgtaaatcgg tcatacgcgt
     5341 tcaatccaac gctccagaag ctccggcaac atcagcgcaa tttcatgaag taactgcgtt
     5401 ccctgccctt gttgatagtg actggcatcg cgactggtaa acagcacgac acccaaatca
     5461 gcatcgcttc ccagcatcga catcgccacc gatcccaccg ctttcgcttc cggtagcacc
     5521 accagcagct ctggtccgtt aagcggccca agatagtgct gttcctgccc caaacgctga
     5581 atacgcagcg gttcgaaaga ctgacggctt aatgccagat gagtgtggtt cgacggcgca
     5641 cctaagcgcc agcgatccgg aaacaggcgc agactcgcac ctgccaggcc gagatcgcgc
     5701 gcccagcggt gaaagcgcat cagcatatcg tcgagactgc tggcggcggt gagactgcgc
     5761 tgcaggtaga gtaggcgata aaacaggcct tcgttggcga tagcctgttc catcaacagc
     5821 gccatgttct cttccagaac atgaatatga ttacgtgcgc gggccatgtg ccactcgacc
     5881 aacgaaacgg tgccgcgtac cggatgcggc acacgtatcg cttctactgc gcgcgcatta
     5941 cggataaaaa actcaggatt tttaatcaga taatcgacaa ccgcccggtc atcaagctcc
     6001 gtgagtgttt cctgcagttc ttcccctggt tgcttcatag atgaataaat ccgtcgtaga
     6061 catgtaccgc cgggccagtc atatataacg ggtgacccgg acctttccag gcgatatcaa
     6121 gacgaccgcc ggggagttcc acgcgtactt cttcggccag caaaccttgc tgaatcccta
     6181 ctgcaaccgc cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc ccacgctcat
     6241 aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata ttggcgcgct
     6301 ccggaaaacg ctcgtggctt tccagaacag gaccaagcgt ttctaccgcc gcggtatcga
     6361 catcatcgac ctgaatcacg caatgcggat ttcccatcga caccacgccg cataagattg
     6421 tctgctcggc ggcgcgcata atataggtct tttccgcttt gttagcgcga aacggcacgg
     6481 cggaaggttc gaagttgggt tcgcccatat ttacgcggac cagatcatca tcggtgacgg
     6541 tcagaaccat ccgcccgttg gcggtgctga cgcggatatc acgcttattg gtcagtcctt
     6601 tcagacgcac aaaacgggca aagcagcgcg caccgttgcc gcactgcgcc acttcactgc
     6661 catcagcatt gaaaatgcga tagtgaaaat ccagttcagg atcatacggc ggctcaacca
     6721 ccagcagttg gtcaaaccct acccccaggt gccgatcagc caggcgacga atcagctccg
     6781 gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat aaaatcgttg ccaaggccat
     6841 gcattttcga gaactgcatc atttactcca atcacgcggg tacagaaact gacttttagt
     6901 aattcacctg ggatggacca tcgccagtgg cgcgatcgtt tttatccggc accgtggatt
     6961 gcgtttgcgt ctctaccggt ttggtcggcg gcggtgcgtt tttatctgca ggcgggaaat
     7021 agagcggacc tttcagaccg cagcccgtca ggctgaagag agtaagtaat acagtgagtg
     7081 ccttaaacac gtttttcatt atggattgcc tgtaaagttc at
//
LOCUS       DAJVRF010000177         7119 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJVRF010000177 DAJVRF010000000
VERSION     DAJVRF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7119
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.177"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            1..613
                     /locus_tag="OQN13_003588"
                     /pseudo
     CDS             1..613
                     /locus_tag="OQN13_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1227..1603)
                     /gene="rnpB"
                     /locus_tag="OQN13_003589"
     ncRNA           complement(1227..1603)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OQN13_003589"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1636..2781)
                     /gene="garK"
                     /locus_tag="OQN13_003590"
     CDS             complement(1636..2781)
                     /gene="garK"
                     /locus_tag="OQN13_003590"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCS1616852.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2878..3768)
                     /gene="garR"
                     /locus_tag="OQN13_003591"
     CDS             complement(2878..3768)
                     /gene="garR"
                     /locus_tag="OQN13_003591"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCS1616853.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3798..4568)
                     /gene="garL"
                     /locus_tag="OQN13_003592"
     CDS             complement(3798..4568)
                     /gene="garL"
                     /locus_tag="OQN13_003592"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCS1616854.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRTNMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(4584..5918)
                     /gene="garP"
                     /locus_tag="OQN13_003593"
     CDS             complement(4584..5918)
                     /gene="garP"
                     /locus_tag="OQN13_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCS1616855.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            6293..>7119
                     /locus_tag="OQN13_003594"
     CDS             6293..>7119
                     /locus_tag="OQN13_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HCS1616856.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHTPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     "
BASE COUNT         1878 a         1881 c         1633 g         1727 t
ORIGIN      
        1 atgttccagg ttaattttgt tagttcaaca caaaacgcca caatctcctg ggattattta
       61 ctaaaaataa cgcctgtttt aataagcatt agcgatatgt attctgaaga aaaaatcaag
      121 tttgtcgaaa gttgtttaaa tgagcttgga gacattaccg aagaacaatt aaaaattatg
      181 agatttgcaa ttataaaatc tataccaagg gcaactctta cagataaatt agaaaatgaa
      241 ttaacaaaag aaatatataa aagctcatcg aaaatcaaca attgcttgaa cagaattaaa
      301 ttaccagaga tgaaagaatt ctcatcagaa aaaaatatat gattacatcg atataatcat
      361 tgaagattat gaaaatatca aagaaaatgc ttatctggtc gatccaaaaa ttaattacac
      421 tatagattta aacatagaag actctagctc agaggaattc ctttttgata atagcctcga
      481 gaaagacgaa aattcttcgg acaattgctt tgaggtcgaa aaatataacg catatgaagc
      541 atataactca gagaatctat attttaccag agaggaatat acgtatgatt acgacctttt
      601 aaatgcaata tagacataat aacttcttac aaaatctata attaataccg tagatattca
      661 ttcatcttac cctgtgcacc aaaagcaaaa actcactgac gcgggtttga aactgatggc
      721 aaccgcaaaa atgcctgatg cgctacgctt atcaggccta cgccatctct acaatatatt
      781 gaatttgcgt gcttttgtag gcaggataag gcgttcacgc agcatccggc atgaataaag
      841 cgcactcaac aatctgaaac ccgccggaac ggtttattac gtacatcagg tgaaactgac
      901 cgataagccg ctttcttttg ggtatagtgt cgtggacagt cattcatctt tctgcccctc
      961 caaaagcaaa aacccgccga agcgggtttt tacgtaaatc aggtgaaact gaccgataag
     1021 ccgctttctt ttgggtatag cgtcgtggac agtcattcat ctttctgccc ctccaaaagc
     1081 aaaaacccgc cgaagcggtt ttttacgtaa accaggtgaa actgaccgat aagccgcttt
     1141 cttttgggta tagcgtcgtg gacagtcatt catctttccg ctcctccaaa agcaaaaacc
     1201 cgccgaagcg ggtttttacg taaatcaggt gaaactgacc gataagccgg gttctgtcgt
     1261 ggacagtcat tcatctaggc cagcaatcgc tcactggctc aagcagccta cccgggttca
     1321 gtacgggccg taccttatga acccctattt ggccttgctc cgggtggagt ttaccgtgcc
     1381 acggactgtt accagccgcg cggtgcgctc ttaccgcacc ctttcaccct tacctgatcc
     1441 cgcttgcgcg ggccatcggc ggtttgctct ctgttgcact ggtcgtgggt ttccccccca
     1501 ggcgttacct ggcaccctgc cctatggagc ccggactttc ctcccctccg cccgtctccc
     1561 ccgaagagga cgacgacgaa gcggcgactg tctggtcagc ttcggcgcgc agtatagagg
     1621 gtttgcgcgc ccttgtcacc ccgcgttgcg cattccaatc gccagtgtcg cggcgatatt
     1681 acgtgaagca cggcagatat tgtcataagc cccgcggaat gcttcgtcca acgtacctat
     1741 gctggtcaat acgctgaaga ccgcatcgat gccatgctga tgtacaacgc caacatcatc
     1801 ggtcaggcta cccgcaatgc caatcaccgg tttatggtac ttcttcgcca cgtttgcaac
     1861 gccaatcggt accttgccgt gaatactctg gctgtcaata cgcccttcac cggtgatcac
     1921 cagcgtacaa tcgtgaatat gttcttccag attcagcgcc gtagtgacga tttcaatacc
     1981 acttttcagt tccgcaccaa gaaacgccat cagcgccgcg cccataccgc ccgcagctcc
     2041 tgcgccgggg acatctttca catcaacatg cagcgctttt ttaatgacat cggcatagtg
     2101 agagaggtta ttgtccagct caacaatcat cgcttcactg gctccctttt gtgggccaaa
     2161 gatgcgcgat gcgccgttat cgcccaccag cggattggtg acatcacaag cgacgcgaat
     2221 gacgcaatct tttaagcgcg gatcgaggcc ggaaatatca atatcattca gagtattaag
     2281 actaccgccg ccaaaaccaa tttcattgcc gttggcgtcg cataatttcg cccccagcgc
     2341 ctgtaccatg cctgcgccgc catcatttgt agcgctgccg ccaatgccga taataatgtt
     2401 tgttgcactg ctctccagcg cctgcaggat taactcgcct gtgccgcgtg aagtggttac
     2461 gagtggatcg cgtttttccg caggtaccag ctccagccca ctggccgccg ccatttcaat
     2521 aaacgcggtt ttgccatcgc cggagatccc ccaactggca ttcactttct cgcccagcgg
     2581 ccctgtaacc caggcgtgac gttcagcccc ctgggtggct gcaatcatcg cttccaccgt
     2641 tccttcacca ccgtcggcaa ccggaacaga aacatactgt gcatcaggaa aaatttcccg
     2701 aaatcctttt tctatcgcct gcgcaacctc gctggcagat aaactttctt tataagagtc
     2761 tggggcgatt acgattttca tacctatgcc tgttaccaca tgacgccgga gggcgtttct
     2821 cttattcggc ctagattcca ggcccggatt gcaatacacc atccgggcac gacgtcatta
     2881 acgagtaact tcgactttcg ccagtttttc gtagtagcac gccagggcgc tatgatccgc
     2941 cgttcctaaa ccatctgcac gcagtgcctg catcatctcc ataaccgcag ctgtgagcag
     3001 cagttgtgcg ccgacgccgt gagaagtatc cagcgcattc gccagatcct taatatgcag
     3061 atcaatgcgg aagcccggct tgaagttgcg gtccatcacc atcggcgctt tggcatccag
     3121 cacggtactg cccgccagcc caccgcgaat tgcctgataa accaggtccg ggttaacgcc
     3181 cgctttggtt gccagcgtta acgcttctga catcgcagca atattcagcg ccacaatgac
     3241 ctgatttgcc agtttggtga cgttacctgc accgatttcc ccggtatgca ccacggaacc
     3301 cgccatcgct ttcatcaaat catagtattt gtcgaaaata gccttgtcgc cgcccaccat
     3361 cactgacagc gtaccgtcga tggctttcgg ttcaccgccg ctcaccggag catccagcat
     3421 atcaatgcct ttcgctttca gcgcttcgct gatttcacgg cttgccagcg gtgcgataga
     3481 actcatatcg atcaataccg tacctggctt cgcgccttca ataatgccat tctcacccag
     3541 cgccacctct ttcacatgag gggagtttgg cagcatggtt atgatgacgt cgcactgttc
     3601 agcgatcgct ttagccgtag acgctgtttc tgcacctgca gcaatcacgt cagcaatagc
     3661 ttctgggtta cggtcagcaa ccaccagcga gtaacctgct ttcagaaggt ttttactcat
     3721 tggtttaccc ataatcccca ggccaataaa accaactttc atagtcatat caaccatctc
     3781 tcttgttgcg gtggtggtta ttttttaaag gtatcagcca gtttctgagt ggcagagcgg
     3841 aagacgccaa gatcgctgcc gacagccaca aacgtcgcgc cccattccag ataacgacgc
     3901 gcatcggctt cgaccggcgc gagaataccg ctgggtttgc cgtgcgcgct ggcacggtta
     3961 aaaatatgct gaattgcttt ttgtacatcc ggatgcgagg cattgccgag atggcccaat
     4021 gccgcagcca gatcgctggg gccgacgaag ataccgtcta caccttcggt agcggcaata
     4081 gcatcgacgt tatctacgcc ctgctgactt tctatctgga ccagaatagt aatgttcttg
     4141 ttcgactgag cgaaataatc cgccacggtg ccaaacatat tggtgcggtg agaaacggag
     4201 acgccgcgaa tgccttccgg cgggtaacga gttgatgcca ccgcctgctc tgcttcctct
     4261 tttgtttcta caaaaggaat caggaagtta tagaaaccga tatccagaag acgcttaata
     4321 attaccggct cgttggtcgg cactcgcact actggcgcgc tggcgctgcc tttcagggcc
     4381 attaactgcg gaataaacgt ggaaatatcg tttggcgcat gttcgccatc cagcaccagc
     4441 cagtcaaacc cagccaaacc aagaacttca gtgctaatcg ggttagagag tgctgaccag
     4501 caaccaattt gtacctgttt cgcagccagt gcggctttga atttattcgg gaaaacatcg
     4561 ttattcatcg cttatacctt tgcttatttc tgcaattcca tacgtttaat gtcgccaact
     4621 acgaagaggt agcagaccat cgccatcagc gctgaacatc ccacgaaaac cagtgctgca
     4681 ttgaaggagt gcagttcact taccaggtag ccaatcacca gtggagtgac aatggaggca
     4741 acattgccaa agacgttaaa gacgccgcca cagaggccaa caatctcttt cggcgcggtg
     4801 tcagaaatca ccggccagcc cagagcacca aatcctttgc caaaaaaagc cagcgccatc
     4861 agcatgacca ccagcgtggt gttgttggtg tagttacata agatgatggt ggaaaccagc
     4921 aacattccca gcacaatcgg tagcttacgt gccagggtca gggataaacc gcgtttgatc
     4981 agataatccg agaagacacc tcccagcacg ccgcccgcaa aaccacacag tgccggaatc
     5041 gaggcgacca gacccacttt cagaatcgac atgccttttt cctgcaccag ataaatcggg
     5101 aaccaggtga ggaagaacca ggtgatggtg ttgataaaat attgtccgaa aaatacgccc
     5161 aacatcatgc ggttagagag caattgcttg atgtaatgca gtttgggacc gcttgctgcc
     5221 gcgctgcccg gctttttgtg gtccatatcg accaccgcgc cattttcaga gataaacttc
     5281 agctcttccg cagacatacg tgggtgatct gtcgggttat gaatcaactt gatccacagc
     5341 gccgtcagca caaaaccaat cccccccata acggtgaaga cgtgctccca gccccaagcg
     5401 aaagtcagcc agccaagcag cggcgaaaag agcgccagcg agaaatattg cgccgagtta
     5461 aagatggcgg aggcagtacc acgttctttc gtcgggaacc aggcggcgac aatacgggcg
     5521 ttcgccggga atgatggcgc ttccgagaac cccagcataa agcgcataaa gaacatggag
     5581 atccctgccc aggccagcgg gaacatatca acaaagcctt gcaggaaggt gaacagcgac
     5641 cagaaaaaga ggctgtaggt gtaaactttt ttcgagccaa acttatcaag caaccagccg
     5701 ccggggattt gcatcagcaa gtaggcccag ccaaaagcag agaagatgta acccatcgaa
     5761 accgcactta actgcaactc ttttgccact tcggtaccag caatagacag cgttgcacga
     5821 tcggcgtagt taacggcggt aacaataaaa ataatcagta atattaaata gctggtatgc
     5881 acgcctttct tttttacgtc aacggtgtcc agaatcattt tatttacctc gggtacttat
     5941 gctgattttt attattatgg ggaaggtgtt atttatgagt tttatttatg ccgttacgac
     6001 aatgaactcg ggaattagta taagcagcgg gagaataata atcattgtgc aaatgctaat
     6061 ttaatcagta ctatttaaat attattttga gcatatgcac ataaggttgc gcgctaaagc
     6121 acagatttgc gctttacctt accgggcggc actgcaatcc ctgaaatgat tgacattgat
     6181 cacatttctg cgtttaaact cctgacattc ttatttcacc caatgaagtc atttattttt
     6241 aaatgagacc agatcctcat tttaataacc cctggctgga gaatattgca caatggccaa
     6301 catcgaaatc agacaagaaa cgccaactgc gttttatata aaagttcacg acacagataa
     6361 tgtggcaatt attgttaatg ataatggcct gaaagcagga acgcgttttc cggatgggct
     6421 ggaattaatt gaacatactc cccaggggca taaagtcgca ttgctggaca ttccagctaa
     6481 tggtgaaatt attcgttatg gcgaagtgat tggttacgcc gtgcgcgcaa tcccacgcgg
     6541 aagctggatc gacgaatcaa tggttgtact accggaagcg ccgccgttac acacgctgcc
     6601 actggcaacc aaagtcccgg cccccttacc gccgctggaa ggatacacct ttgagggcta
     6661 tcgcaatgcc gatggcagcg tgggcaccaa aaacctgctc ggcatcacca ccagcgtcca
     6721 ctgtgtggca ggcgtggtgg actatgtagt aaaaatcatt gaacgcgatc tgctaccgaa
     6781 atacccgaac gtcgatggcg tggtggggct gaatcatttg tacggctgtg gcgtggcgat
     6841 taacgcaccg gcggcagttg tgcctattcg taccatccac aatatttcgc tgaaccctaa
     6901 ctttggcggc gaagtaatgg tgattggtct gggttgtgaa aagttgcagc ctgagcgcct
     6961 gctggtcggc acggatgatg tgcaagctat tccagtagaa agcgccagca ttgtcagttt
     7021 gcaggatgaa aagcatgtcg gctttcagtc catggtcgag gatattttgc aggtcgccga
     7081 acgccatcta caaaaactga atcaacggca gcgagaaac
//
LOCUS       DAJVRF010000178         6661 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJVRF010000178 DAJVRF010000000
VERSION     DAJVRF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6661
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.178"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..339
                     /locus_tag="OQN13_003595"
     CDS             <1..339
                     /locus_tag="OQN13_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616857.1"
                     /translation="DELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVRVDEKER
                     QVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIERNAKAR
                     IRRATKSRTE"
     gene            432..650
                     /locus_tag="OQN13_003596"
     CDS             432..650
                     /locus_tag="OQN13_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCS1616858.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKEN"
     gene            805..1038
                     /locus_tag="OQN13_003597"
     CDS             805..1038
                     /locus_tag="OQN13_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001726830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1616859.1"
                     /translation="MVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQRSLSERLFRE
                     QGVDTKILLGHSNQKMTDIYNDVRGKEWKKLVI"
     gene            complement(1071..1709)
                     /gene="ompW"
                     /locus_tag="OQN13_003598"
     CDS             complement(1071..1709)
                     /gene="ompW"
                     /locus_tag="OQN13_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCS1616860.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1949..2038
                     /locus_tag="OQN13_003599"
                     /pseudo
     CDS             1949..2038
                     /locus_tag="OQN13_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2066..2809
                     /locus_tag="OQN13_003600"
     CDS             2066..2809
                     /locus_tag="OQN13_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCS1616861.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2839..3378
                     /locus_tag="OQN13_003601"
     CDS             2839..3378
                     /locus_tag="OQN13_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCS1616862.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3483..3881
                     /gene="yciA"
                     /locus_tag="OQN13_003602"
     CDS             3483..3881
                     /gene="yciA"
                     /locus_tag="OQN13_003602"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCS1616863.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3921..4640)
                     /gene="tonB"
                     /locus_tag="OQN13_003603"
     CDS             complement(3921..4640)
                     /gene="tonB"
                     /locus_tag="OQN13_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCS1616864.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4731..6251
                     /locus_tag="OQN13_003604"
     CDS             4731..6251
                     /locus_tag="OQN13_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1616865.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6284..>6661)
                     /locus_tag="OQN13_003605"
     CDS             complement(6284..>6661)
                     /locus_tag="OQN13_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616866.1"
                     /translation="ANGLRIQMTFQGVEFRSIVGQTDVSAATGKTATTNDGGATSKKN
                     TGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSVKNGESTFSDAANEQA
                     SKVLKPGNALRKYSVNPNKKGTY"
BASE COUNT         1750 a         1527 c         1595 g         1789 t
ORIGIN      
        1 gatgagttaa gcaatatcgt tttatttccg gtaaaagagg atgacccacg taatcaggtt
       61 aattttcttt atgaaccatc ggaaagacca tattgccatc acgcctctgt ccgggttgac
      121 gaaaaagagc gtcaggtccg ctgtaaaatc tgcggtgcgg ttgtggagcc gtttgactgg
      181 atgctctctg tggcaaaaag agaaaccaga ctggcagatg atgtaaggct attgcgccag
      241 gaggaacagg aaaggcggag aaatatagaa aagctgatac agattgagcg taacgcgaaa
      301 gcgcggatac gcagggcgac aaaatccaga actgaataat taaatttagc actgttaaaa
      361 atttaatcct taaccggagg gatttctgca ccctcaaatc atcaggagac cgcccgaaag
      421 ggcggtaatg aatggttaca ttatttagaa aaaaatatcc gcgaaagagt agaacaacag
      481 aattcctgtt tctcattctg tttatcgtgt tgatgacacc gatatccccg ctaatttttg
      541 tctggataat cggaaaaata attgagccag ttattgaatt gtataacgac gtggtatggg
      601 cgtcattcaa cacactgcac aataaaatta atccgtataa ggaaaactga tatggcaact
      661 ttgacaaaaa aagaacgggc atggttgaac gaattacagg aagttcttga tcgctgtcct
      721 gtggtgtcat cctgccgcga ttgcgttctt agtccgtggc tattgcacca ccatcacgcg
      781 aaagggacag ctaagcgcgg cgggatggtt aagccagcaa cattaaccgt tgcatttaaa
      841 aaagcccggg attctgtgga ttacaactgg cgtgctaatg gcaccccacc ctctttccat
      901 gagcagagat ctttatcaga gcgattgttc agagagcagg gggttgatac caaaattttg
      961 ctaggccatt cgaatcaaaa aatgaccgat atttacaacg atgtacgcgg taaggagtgg
     1021 aaaaaactgg tcatttgata accagttttg cagaggtttt gcagagaaaa ttaaaagcgg
     1081 tacccggcag agaacataaa cacccacgga tccagtcgta ccgagtcttt tacggtagta
     1141 acaccagatt tgtacttagc cgtggtatcg atatccatgt accacactga catgttaacc
     1201 aaccagtcac ggttaatcag ataatcaacc cccacctgcc cggcagctcc ccagtaatct
     1261 ttcagactga gatcggaaag ccccgcctct ttgccatgat cgttaaatcc attatcaaag
     1321 aaggtggtgt agttaatacc tgccccaaca taaggacgga atttgctgcc ggcatcacca
     1381 aaataccact gcgccatcag tgttggcggc agatgatgaa cggttgcaat atcgccggtc
     1441 gcccgggtgc cgattttatg gcggaacggc gtcgctgcca gtaattccac accaatgttg
     1501 tcggtcgcca tataagtaaa cgtcaggccc agttgcgtgt tattggtcac gctgaatcca
     1561 cccagacttc ctaacgtacc accagcacct tctgttggac gtacggttgc agaacctgca
     1621 cgcataaaaa attcgcctgc ttcatgcgca aaggcactgc cagagagaag agttgttact
     1681 gccaaagccg ccactgttaa ctttttcata tccgctccgt cgttatggtt ataaaaacgt
     1741 gacgaatata cctacattgg agtaacaaat gatcctacat agatcacatt acatatgata
     1801 attcaccctt tattgatctg gattaattta gaagttgcat tgagaatcct ggttaacgtg
     1861 atttaaatca atttttagat attttcgcta cgcaaatatt aactcgttcg ccacttgccg
     1921 cggtttgccc acgcctgaag gccagcaatt acctccgctt tacaaagata tgcttttcca
     1981 tacaagacct gatgctgcca cccgaggctt aaccagggta caattgcccg ctaattaaca
     2041 cctgcataaa actcaaggag agtgcatgtc tatcacggcg cagtccgtat accgtgacac
     2101 cggaaatttc ttccgtaatc aatttatgac cattctgttg gtatcgttgc tatgtgcgtt
     2161 tatcacagtg gtgttagggc atgttttctc acccagtgat gcacagcttg cgcagctcaa
     2221 tgacggcgtg cccgttagcg gcagtagtgg gttgttcgac ctggttcaga atatgtcacc
     2281 ggaacaacaa caaattttgc tgcaggcttc agcggcgtcc actttttcag gattaatcgg
     2341 taacgccatt ctcgccggag gcgtaatatt aattatccag ctggtgtctg cgggtcagag
     2401 agtcagtgcg ctgcgagcta ttggtgccag tgcgccgata ttgcccaagt tatttattct
     2461 gatttttctg actacccttt tagtacagat tggcatcatg ctggtggtcg ttccgggaat
     2521 tatcatggcc attttactgg ccctggcacc ggtgatgctg gtacaggaca aaatgggcat
     2581 ttttgcctcg atgcgtagca gtatgcggct gacttgggcg aatatgcgtc tggtggcacc
     2641 cgcagtactg agctggttgc tggcaaaaac actgttgctg ctttttgcct cttcttttgc
     2701 cgcattaacc ccggaaattg gtgccgtact ggcgaacacc ttgagcaacc tgatttcagc
     2761 catattgctc atctatctgt tccgcctgta tatgttgatt cgccaataac cttaagcatt
     2821 tgattacgga atcgtaaaat gaagcagttt cttgattttt taccgctggt tgtctttttc
     2881 gcgttttaca agatttatga catctatgcg gctactgcgg cgctgatcgt cgccacggcg
     2941 attgtgctta tctatagctg ggttcgcttt cgtaaggttg agaagatggc cctgatcact
     3001 tttgttctgg tggtcgtctt cggtggcttg acgctgttct tccacaatga tgagtttatt
     3061 aaatggaagg ttacagtcat ttatgctctg tttgcgggag ccctgttagt cagccaatgg
     3121 gtgatgaaaa agccgctaat tcagcggatg ctgggtaaag aactcacgct gccgcaaccg
     3181 gtatggtcga agctaaatct ggcctgggct gttttcttta tcctttgcgg tctggcaaac
     3241 atctacatcg cattctggct gccgcaaaat atttgggtca actttaaagt ctttggcctg
     3301 accgccctta ccttaatctt cacattgtta agcggtatct atatctaccg ccacatgccg
     3361 caggaagata aatcctaacc agactgccag tccttcgggg ctggcgctgt atctcctctg
     3421 ttctcctcag gaaaatcata gtagcatcgc gcctgtgatt ttccttttaa gtcggtttta
     3481 ccatgtctac aacacataac gtccctcagg gcgatcttgt tttacgtact ttagccatgc
     3541 ccgccgatac caatgccaat ggtgacatct ttggtggttg gttaatgtca caaatggata
     3601 ttggcggcgc tattctggca aaagaaattg cccacggtcg cgtagtgact gtgcgggttg
     3661 aaggaatgac tttcttacgg ccggttgcgg tcggcgatgt ggtgtgctgc tatgcacgct
     3721 gtgtccagaa agggacgaca tcggtcagca ttaatattga agtgtgggtg aaaaaagtag
     3781 cgtctgaacc aatcgggcaa cgctataaag cgacagaagc attatttaag tatgtcgcgg
     3841 ttgatcctga aggaaaacct cgcgccttac ctgttgagta atagtcaaaa gcctccggtc
     3901 ggaggctttt gactttctac ttactgaatt tcggtggtgc cgttaatttt aaacaggata
     3961 ttcaccacaa tcccactgcc tggcttaccc ggctcataac gccatctgcg catcgcattt
     4021 ttcacctcac gctcaaacat gttcgcaggc ttggctgaga ggatttgtac gttatccacg
     4081 cgaccatccg gcgtgacgtc aaatttaact ttaacctgcc cttcaatgcg caatgcctgt
     4141 gctcgtgccg gatactgcgg ctgattacgg cttaatgcgc gtggtcctga agccacactg
     4201 gtaaccggct tgctggttgc agccgttgcc gtacttgatg tggggcgcgc gggtgccgta
     4261 ttttcaaacg gtgatgccgg acgcgactct acgggtttga catcgcgttt tggctgctcc
     4321 tgtacctttt tcaccggctt cggttttggc ttaggtttcg gcttcggctt ttcaatgacc
     4381 accggtgctt cttttggcgg ttcggggatc ggctcaggtt ccggttctgg ctctaccacc
     4441 ggctccggtg gcggctgaac ggcttgtggc ggttcgagat cagcaggcgc aaccatcgtg
     4501 acagaaattg gctgcgcagg cgcaggtagt tcaataacct gatgtaccga ggtatagagc
     4561 agacccgcca caacagcacc atgaatgcag accgaaagta acgtcggcca ggggaagcgg
     4621 cgaggtaaat caagggtcat tgtattgtag ctattcggca ttgtagcatt agccataacg
     4681 tttataagtt catctttaaa tgataatcaa ttgcatggga gtttatcacc gtgagaaccg
     4741 taataacata cttgagattc tcatcagcca tacaaggcgc tgaaggtgca gattcaacca
     4801 gacgacaaaa cgacctgttc aagcagtggc tgaagaagaa tggcgatgct caaatagtag
     4861 cgtctttcag tgatgaaggg ttgagtggtt acaaaggtaa acaccttact ggtcagtttg
     4921 gtgacatgtt agcccgtatt gaggctgggg agtttccaga aggtacaatt ctgctagttg
     4981 agagtatcga ccgtataggc cgattagagc atcttgaaac agaagccttg atgaaccgca
     5041 tcttaggcaa cgggatagag attcacacat tgcaagatgg ccttatctac acaaaggatg
     5101 ctttagccga tgaccttggc ataagcatca tccagcgtgt caaagcctac atagcgcatc
     5161 agaaatctaa gcagaagtct ttccgtgttt ctcagaaatg ggggcaacgt gcaaaacttg
     5221 cccttgctgg tgaacaacgt cttacaaaga tggttcctgg atggattgac cctgagactt
     5281 ttaaactcaa tgaacacgct gagactgtga gattgatatt caaactgctg ctagatggtg
     5341 aaagtctgca taacattgca cgtcaccttc aaagcaacgg tataaagtcg tttagtcgcc
     5401 gtaaagatgc taatgggttc tctgttcact ctgtacgcac cattctaagg tcagagacaa
     5461 caataggcac gttaccagca tcacaacgta atgaccgccc cgctataccg aactactacg
     5521 aaggtgttgt agatatacca acgttcaata aagctcaaga gattctcgac aagaatcgta
     5581 aaggccgtac acctgcaagt gacaacccac taacgattaa catcttcaaa ggtctgttta
     5641 ggtgtcagtg tggggctagt gtccatccta ccggaacaaa gaataagtat gctggggtct
     5701 acaggtgcaa taaccactta gacggtcgct gtgatgttcc accgttgaag cgtaaaccgt
     5761 ttgaccgatg gatgattgat aattttctgg ggatgattga cgtggggaat gatggagaat
     5821 cagagagaaa gattgcagcg ttacagcatg aggttgaaat tgtcacagcc agaatcaaga
     5881 aagctaccgc cctacttctt gagatggatg atattgatga actaaaaatt cagcttaagg
     5941 aactgaacca gaagcgcaca gaactacaga ccacgattga taacatgagg cgtaaggctt
     6001 cactcactga taaggaacta ccccagctta aagatattga ccttatgact aaagcgggtc
     6061 gtgtcgagtg tcagttgatt ttgtccaagc atctaaaagg gcttacattg ggtaaggatt
     6121 cggtaactgt aacgctacag aacgacactg aaatcaccat acctacaaac ccgttaccgc
     6181 taaatgatgg gtcaccaatc tttgaaattg ctgataaaga gctactggat attgacgctt
     6241 atcaactgta attttcaggg gctatgaagc ccctttttat gtctcaataa gtgcccttct
     6301 tgttagggtt cacagagtac tttcgcagag cattacccgg cttaagcacc tttgaagctt
     6361 gctcattagc ggcatctgag aaggtgctct caccattttt aacgctaacg ctcttagagc
     6421 acgttacacg ggcttttagc gcttcatctg ataactcact tgccgaatag ccaaaaagct
     6481 ctttacaggt cagcataggg cttccaaggc tcgttgtagt gtttccggtg ttctttttac
     6541 tggtagcacc gccatcattc gtggtagcgg tctttccagt agcagccgaa acatcagtct
     6601 gacctacgat tgaacgaaac tctacacctt ggaaggtcat ctgaatacga agaccgttag
     6661 c
//
LOCUS       DAJVRF010000179         6646 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJVRF010000179 DAJVRF010000000
VERSION     DAJVRF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6646
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.179"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            16..762
                     /gene="glnH"
                     /locus_tag="OQN13_003606"
     CDS             16..762
                     /gene="glnH"
                     /locus_tag="OQN13_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCS1616867.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            901..1560
                     /gene="glnP"
                     /locus_tag="OQN13_003607"
     CDS             901..1560
                     /gene="glnP"
                     /locus_tag="OQN13_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCS1616868.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            1557..2279
                     /gene="glnQ"
                     /locus_tag="OQN13_003608"
     CDS             1557..2279
                     /gene="glnQ"
                     /locus_tag="OQN13_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCS1616869.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            2396..4621
                     /gene="ybiO"
                     /locus_tag="OQN13_003609"
     CDS             2396..4621
                     /gene="ybiO"
                     /locus_tag="OQN13_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCS1616870.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(4618..5544)
                     /gene="rlmF"
                     /locus_tag="OQN13_003610"
     CDS             complement(4618..5544)
                     /gene="rlmF"
                     /locus_tag="OQN13_003610"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCS1616871.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            5820..6080
                     /locus_tag="OQN13_003611"
     CDS             5820..6080
                     /locus_tag="OQN13_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1616872.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            6345..>6646
                     /locus_tag="OQN13_003612"
     CDS             6345..>6646
                     /locus_tag="OQN13_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCS1616873.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPGV"
BASE COUNT         1566 a         1722 c         1711 g         1647 t
ORIGIN      
        1 aaaaaaggaa atgctatgaa gtctgtatta aaagtttcac tggctgcact gaccctggct
       61 tttgcggttt cttctcatgc cgcggataaa aaattagttg tcgcgacgga taccgccttc
      121 gttccgtttg aatttaaaca gggcgataaa tatgtgggct ttgacgttga tctgtgggct
      181 gccatcgcta aagagctgaa gctggattac gaactgaagc cgatggattt cagtgggatc
      241 attccggcac tgcaaaccaa aaacgtcgat ctggcgctgg cgggcattac catcaccgac
      301 gagcgtaaaa aagcgatcga tttctctgac ggctactaca aaagcggcct gttagtgatg
      361 gtgaaagcta acaataacga tgtgaaaagc gtgaaagatc tcgacgggaa agtggttgct
      421 gttaagagcg gtactggctc cgttgattac gcgaaagcaa acatcaaaac taaagatctg
      481 cgtcagttcc cgaacatcga taacgcctat atggaactgg gcaccaaccg cgcagacgcc
      541 gttctgcacg atacgccaaa cattctgtac ttcatcaaaa ccgccggtaa cggtcagttc
      601 aaagcggtag gtgactctct ggaagcgcag caatacggta ttgcgttccc gaaaggtagc
      661 gacgagctgc gtgacaaagt caacggcgcg ttgaaaaccc tgcgcgagaa cggaacttac
      721 aacgaaatct acaaaaaatg gttcggtact gaaccgaaat aataacgcta cacctgtaaa
      781 gcgcactggc agttccctct cccctctggg gagaggatta gggtgagggg cgcaaacccg
      841 ctccggggcc attaattacc ctgaatttga ttatttacac cacggtaaca ggaacaacat
      901 atgcagtttg actggagtgc catctggcct gccattccgc ttctgattga aggtgccaaa
      961 atgaccctgt ggatttcggt cctcggtctg gctggcggtc tggtaatcgg attgctggca
     1021 ggttttgcac gcaccttcgg tggttggata gccaaccacg tcgcgctggt ctttattgag
     1081 gtgatccgcg gcacacctat cgtcgtccag gtgatgttta tttatttcgc cctgccgatg
     1141 gcgtttaacg acttacgcat cgacccattt actgcggcgg tggtcaccat catgatcaac
     1201 tccggcgcgt acattgcgga aatcacgcgt ggtgcggtgc tgtctattca caaaggtttt
     1261 cgtgaagcag gactggcgct cggtctttca cgttgggaaa ccattcgcta cgtcatttta
     1321 ccgctggcac tgcgtcgtat gctgccgccg ctgggtaacc agtggatcat cagcattaaa
     1381 gacacctcgc tgtttattgt gatcggcgtg gcggaactga cccgtcaggg gcaagaaatt
     1441 attgccggta acttccgcgc ccttgagatc tggagcgccg tggcggtgtt ctatctgatt
     1501 attaccctgg tgctaagctt tattttgcgt cgtctggaaa gaaggatgaa aatcctgtga
     1561 ttgaatttaa aaacgtctcc aagcactttg gcccaaccca ggtgcttcac aatatcgatt
     1621 tgaacattgc ccagggcgaa gtcgtggtga ttatcgggcc gtccggttcc ggtaaatcga
     1681 ccctgctgcg ctgcatcaac aaactggaag aaatcacctc cggcgatctg attgtcgatg
     1741 gcctgaaggt taacgatccg aaagttgacg agcgcctgat tcgccaggaa gcaggtatgg
     1801 tgttccagca gttttacctc ttcccgcatc tgacggcgct ggaaaacgtc atgtttggtc
     1861 cgctacgcgt gcgtggcgcg aacaaagaag aggcggaaaa actggcgcgt gagctgctgg
     1921 cgaaagtcgg tctggcagaa cgtgcacatc actacccttc cgaactttct ggtggtcaac
     1981 agcagcgtgt ggcgattgcc cgcgcgctgg cggtgaagcc gaaaatgatg ctgtttgatg
     2041 aaccgacttc cgctcttgac ccggaactgc gccatgaagt gctgaaggtt atgcaggatc
     2101 tggctgaaga agggatgacg atggtgatcg tgacccacga aatcggtttt gccgagaaag
     2161 tagcttcgcg gctgatcttt atcgacaaag gccggattgc ggaagatggc gatccgcagg
     2221 tattgatcaa gaacccgccg agccagcgct tgcaggaatt tttgcagcac gtctcttaac
     2281 ttctctgcct ccctttcacc cgaaagggag gcacaccaga ttcctctcat ttaaaatcgc
     2341 ccctccccca gcatctatac ttatcttttt gctctgtttt ctcactggag gagtcatgcg
     2401 gtggatcctg ttcatcctct tctgcctgct gggcgcacct gcccacgcgg tatccatacc
     2461 cggcgttaca accacaacga caacggactc aacgactgaa ccggccccgg aaccggatat
     2521 cgaacaaaaa aaagcggcct atggcgcact ggcggatgtg ctggataacg acacctcgcg
     2581 taaagaattg atcgaccagt tgcgtactgt cgccgcgacg ccccctgcgg aaccggtccc
     2641 gaagatcgtg ccgccgacgc tggttgaaga gcaaaccgtg ctgcaaaagg tcaccgaagt
     2701 cagccgccat tatggtgaag ccctttccgc ccgcttcggg caactttatc gcaatatcac
     2761 cggctccccg cataagccat ttaatccaca aaccttcagc aatgcgctga gccatttttc
     2821 aatgttagcg gtattagtgt ttggttttta ctggctgatt cgcctgtgcg cactgccgct
     2881 gtatcgcaaa atgggtcagt gggcgaggca aaaaaatcgt gagcgcagca actggttgca
     2941 gcttccggcg atgattatcg gggcgtttat tatcgacctg ctgttactgg ccctgacatt
     3001 atttgtcggc caggtattaa gcgacaacct gaatgcaggc agtcgcacca tcgctttcca
     3061 acaaagtttg tttctcaacg cctttgccct gattgaattt ttcaaagccg tactacgcct
     3121 gattttttgc ccaaacgtgg cggagctgcg cccgttcacg attcaggacg agagcgcccg
     3181 ttactggagt cgtcgcctga gctggttaag cagtttgata ggttatggcc tgattgtggc
     3241 cgtgccgatt atctctaatc aggtgaatgt acagataggt gcgctggcga acgtcatcat
     3301 tatgctgtgc atgaccgtct gggcgttgta cctgatcttt cgtaataaaa aagagattac
     3361 ccagcatttg ctcaactttg cggagcattc gctggccttt ttcagcctgt ttatccgcgc
     3421 ctttgcgctg gtgtggcact ggctggcaag cgcctatttt atcgtgctgt ttttcttttc
     3481 gttgttcgat ccgggcaaca gcctgaaatt tatgatgggt gcaacggtgc gcagcctggc
     3541 gattattggt atcgcagcgt ttgtttccgg tatgttttcc cgctggctgg caaaaaccat
     3601 cactctctcg ccacatactc agcgtaacta tccagagttg caaaaacggc tgaatggctg
     3661 gctttcggcg gcgctgaaaa cggcgcgtat tctgaccgtc tgcgtggcgg taatgttgct
     3721 gttgagcgca tggggattgt tcgatttctg gaactggctg caaaacggcg cggggcagaa
     3781 aaccgtagat atcctgatcc gtatcgcact tattcttttt ttctcggcgg ttggctggac
     3841 ggtgctcgcc agtttgatcg aaaaccggct ggcttcggat attcatggcc gcccgctacc
     3901 cagcgcccgt acgcgtaccc tgctgacgct gttccgtaac gcgctggcgg tgattatcag
     3961 taccatcacc atcatgattg tgttgtcgga aatcggcgtc aatatcgcgc cattgctggc
     4021 aggtgccggg gcattaggtc tggctatctc gtttggttcg caaacgctgg tgaaagatat
     4081 cattaccggg gtgtttattc agtttgaaaa cggcatgaac actggagatt tggtgactat
     4141 cgggccgttg accggcacag tggaacggat gtcgattcgc tccgtgggcg tgcgccagga
     4201 taccggggcg tatcacatca ttccgtggtc ttcgataacc acctttgcta acttcgtccg
     4261 cggtattggt tcggtagtgg caaactacga tgttgatcgc catgaagatg ctgataaagc
     4321 caatcaggca ctgaaagatg cggtagcgga attaatggaa aacgaagaaa ttcgcgggct
     4381 gattattggt gaaccgaatt ttgccgggat tgtcggctta agcaataccg cgtttacact
     4441 gcgtgtttcg ttcaccacgc tgccactcaa acagtggacg gtacgctttg ccctcgacag
     4501 ccaggtgaaa aaacatttcg acctggcggg cgttcgcgcg ccagtgcaga cttatcaggt
     4561 gctgcctact ccgggcgcga ccccggctga accgttaccg ccgggggagc caacgcttta
     4621 acgctggcga ttgacaaaac ggcggcgctg ctcgtcgttc ataaaggtcc aggcaataaa
     4681 gcgactctgc ttttgcccct gggccatctc tttttttacc accttcaccg cgcccacgtc
     4741 ggtcagagca cgatacaacg gcggtaagtt ttcaccacga gataccagcg aggtaaacca
     4801 catcacctgc ttcgcgaagc ctttgctctc ttcaatcatc tttttgataa aggcgacttc
     4861 accaccttca caccacaact cctgttgctg accaccaaag ttcagtgcat cgtctttgtt
     4921 cagccccagg ttacggcgtt tacgctcact acctgcccgc gctgcagcgg cggaatcgtg
     4981 gaatggcggg ttacacaagg tcgcgtcgta ttgctcgttt ttatggatga tgccgttaaa
     5041 aattgcccca ctctcttttt gccgacgcag acgaatggcg cggttaagcc ccggattagc
     5101 actgataatc gcctgcgcac tgcttaacgc ctggctgctg gtttcactac cggtaaaacg
     5161 ccagccatat tcatgtacgc caatcagcgg ataaatacag ttcgcaccaa cgccgatatc
     5221 cagaatgctg gcattcgctg gaattgttcc gcttgcttca gccagtaaat cggcaaggtg
     5281 atgaatataa tccgcccggc ccggtactgg tgggcagaga aaaccgtcgg ggatatccca
     5341 gttcgctacg gcgtaaaaat gggccagcaa cgccttattg agcgccttca ccgccagcgg
     5401 attggcaaag tctacgcttt gctccccggc gggtgtaagc gtgaggaatt gcctgagttc
     5461 aggattgacc tgacaaagcg tggcgagatc gtagcggctg tgatgacggt tgcgcggatg
     5521 caaccccggt ttctgggcgg acatagcatt ctcctttaag cagcggcgta agatacccgc
     5581 ataacgcgac agggtaaata tctggctgcg tttatcattc cccaaaacct taacatggct
     5641 atggtttatg gcaattcatc ataaaaatca gcattatgca tgtcattaat ttgtcttatt
     5701 ttgtaaaaat atatcgttca aaaaataagc acatctctgt caattttttg cgtcatttgc
     5761 atcgcgcagg cgacctacac ttatcaggca ctacccgttg tatttatgag gatgtcatta
     5821 tgaaaaagtg cctcacacta ctgattgcca ccgtcctgag cggaatctct ctcacggctt
     5881 atgccgcgca accgatgagt aacctggaca gcggtcagct acggcccgcc ggtaccgttt
     5941 cggcaactgg cgcatcaaac ctaagcgatc tggaggataa actggcagaa aaagcgcgcg
     6001 aacaaggcgc gaaaggttat gtcattaatt ccgcaggcgg aaatgaccag atgttcggta
     6061 ctgcaaccat ctacaaataa ccccaccaac gtaactgcat tcgcctctgg atgcagtttt
     6121 cgcctgtttt gtaatcaaaa aacatcaaat atgaactcaa tgtaaataaa tgtatttctt
     6181 tttcgcgcaa tgggtgatag aaaatcgcgc caagtgataa tgcttatcaa aattattatc
     6241 acttgcatga acactatcac gggattaaca gtggcatcgc atccgcagag atgctttctc
     6301 gtggcagtga aaatttcaac atataagaaa aagtcacctg caaaatggaa aacaatcgca
     6361 atttccctgc cagacaattt cattcgctca cgttctttgc cggtctttgt attggcatca
     6421 cgcctgtggc tcaggcactc gccgccgaag ggcaaactaa cgcggatgac acgctggttg
     6481 tcgaagcatc aacgccttcg ctttatgcgc cacaacaatc tgccgatccg aaattctcgc
     6541 gtccggtagc ggatactacc cgcacgatga cggtgatttc tgaacaagtg attaaagatc
     6601 agggcgcaac caaccttacc gacgcgctca aaaacgcccc cggcgt
//
LOCUS       DAJVRF010000180         6592 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJVRF010000180 DAJVRF010000000
VERSION     DAJVRF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6592
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.180"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..941
                     /locus_tag="OQN13_003613"
     CDS             <1..941
                     /locus_tag="OQN13_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1616874.1"
                     /translation="MLGEKRLVSSVTKEDLLFVRRDLLTGYQKLSNGKISSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRHQ
                     QTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKVG"
     gene            977..1663
                     /gene="mlrA"
                     /locus_tag="OQN13_003614"
     CDS             977..1663
                     /gene="mlrA"
                     /locus_tag="OQN13_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCS1616875.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1723..1830
                     /locus_tag="OQN13_003615"
     CDS             1723..1830
                     /locus_tag="OQN13_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCS1616876.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1811..2542)
                     /gene="yehW"
                     /locus_tag="OQN13_003616"
     CDS             complement(1811..2542)
                     /gene="yehW"
                     /locus_tag="OQN13_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCS1616877.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2547..3473)
                     /gene="yehX"
                     /locus_tag="OQN13_003617"
     CDS             complement(2547..3473)
                     /gene="yehX"
                     /locus_tag="OQN13_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCS1616878.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3466..4623)
                     /gene="yehY"
                     /locus_tag="OQN13_003618"
     CDS             complement(3466..4623)
                     /gene="yehY"
                     /locus_tag="OQN13_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCS1616879.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4630..5547)
                     /gene="osmF"
                     /locus_tag="OQN13_003619"
     CDS             complement(4630..5547)
                     /gene="osmF"
                     /locus_tag="OQN13_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCS1616880.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5799..>6592)
                     /locus_tag="OQN13_003620"
     CDS             complement(5799..>6592)
                     /locus_tag="OQN13_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HCS1616881.1"
                     /translation="DVVVAVVGEAQGMAHEASSRTDITIPQSQRDLITALKATGKPLV
                     LVLMNGRPLALVKEDQQADAILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVG
                     QIPVYYSHLNTGRPYNADKPNKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPT
                     MKRDGKVTASVQVTNTGKREGATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQT
                     VSFPIDIEALKFWNQQMKYDAEPGKFNVFIGTDSARVKKGEFELL"
BASE COUNT         1687 a         1845 c         1629 g         1431 t
ORIGIN      
        1 caatgttagg tgaaaaaaga ctggtttcat ctgtaacaaa agaggatttg cttttcgtaa
       61 ggagagatct gttgaccggt tatcaaaagc tttctaacgg aaaaatttct tccataaaag
      121 gacgctcagt ggtcacagta aactactata tgacaaccat agctggaatg tttcaatttg
      181 caacagataa tggttataca tcaggaaatc catttaacgg tctgacacca ttaaaaaagt
      241 ccaagataga accagatccc ctcacccgtg acgaatttat tcgttttatt gaggcttgcc
      301 gtcatcaaca aacaaaaaac ctgtggatta tcgctgtata cacgggtatt cgtcacgggg
      361 agctggtatc actggcctgg gaagatatag accttaaagc gaggactata accatccgcc
      421 ggaattatac aaaactaggc gaattcactc caccaaaaac tgatgctggt accggaagaa
      481 cgattcatct agttcaacca gctattgatg ctcttaaaag ccaagcggaa atgaccatgc
      541 ttggaaaaca gcattctgta gaggtaaagc agagggaata tgggagaagt actgtgcata
      601 aatgcacttt tgtttttagt cctcaggtaa taaaacagcg gcagttttcc ggaccgcact
      661 ataaggttga ctccatcagg gagtcatgga caagtatctt aaaacgcgca ggtctgagac
      721 acagaaaatc gtaccaatcc aggcatactt atgcatgctg gtcacttgcc gctggagcta
      781 atcctagttt tatcgcaagc cagatgggcc acacaaacgc acaaatggta ttcaatgttt
      841 acggagcatg gatgaaagac aacaatcacg aacagataga actccttaac aaaagactat
      901 ctgaaagtgt cccatgtatg ccccataaga aagtggggta aaataaaaac ttgtaaaatc
      961 agttagttta cccttaatcc ctgtcacgtt acgcgcgtgg cagaggcgtt acgggttgct
     1021 gaaaccgcaa cggacagacg gcggtcatcg actgttcaac gatgccgata ttgaccggat
     1081 ccgcgagatc aaacgctgga tcgacaacgg cgtgcaggtc agcaaagtta aaatgctgct
     1141 cagtaatgaa aatgttgatg tgcagaacgg ctggcgcgat cagcaagaaa cattactgac
     1201 ctacctgcaa agcggcaatc tgcatagcct gcgaacgtgg atcaaagagc gcggtcagga
     1261 ttaccccgcc cagacactca ccacacatct gtttattcct ctgcgccgac ggcttcagtg
     1321 ccaacaaccg actctccagg cgctgctggc gatcctcgac ggcgtactga tcaactacat
     1381 cgccatttgt ctggcttcgg cacgtaaaaa acagggtaaa gatgcgctgg tggttggctg
     1441 gaatattcag gataccaccc gtctgtggct ggagggctgg attgccagtc aacaaggatg
     1501 gcgcattgat gtcctcgccc actcgctcaa tcaactacgc cctgaactat tcgaaggccg
     1561 tacattgctg gtgtggtgcg gtgaaaatcg aacctccgcc caacagcagc aactcaccag
     1621 ttggcaagaa caaggccatg atattttccc actcggcatt taatgattcg ttaacaaatg
     1681 cgctttactg tacaatcctt tcgttaacat aaggagtgca atatgcgcat agctaaaatt
     1741 ggggtcatcg ccctgttcct gtttatggcg ttaggcggaa ttggtggcgt catgctcgca
     1801 ggttatacct ttattttgcg tgctggctaa gcgcctgcac cagcctttca aacaggcggt
     1861 ctgcgatgat cgccgccagt gccaccagta acgccccctg gatcacatac gcggtattaa
     1921 atccgctaag cccgatgatg atgggcgtac ccagcgtgct ggcccctacc gttgaggcga
     1981 tcgtcgccgt accaatgttg ataatcaccg aagttcgcac gcccgccaga atcaccggag
     2041 ccgccagcgg tagctcgacc ttacgcagtc gctgaccacg actcattccc atacctttcg
     2101 caacttctgt cacgctggca tcaatcgctc ccagcccggc aagtgtcgcc tgcaggacgg
     2161 gcagcacacc gtaaaggatc aaggcgataa tcgctggttg cagaccaaag ccgatcaccg
     2221 gaacggcaat cgccagcact gcgacaggcg gaaaagtctg tccaacggcg gcaatagttt
     2281 ccaccagtgg gcgaaattcc gcgccccacg ggcgagtgac agcaattccg gcaccagtgc
     2341 caatgatcac cgcaaacaaa ctcgaaattc ccaccagcca gaaatgagcc agtgccagag
     2401 ctgcaaaact ttcttgctga taaacgggtc gtggcagttg tgggaacaag gcagcaaaca
     2461 gcggctggct gtaaggcagc cagaaaatca gtgccacaaa caaagcaatg agccagaaca
     2521 gcggatcgcg caacatcttc atacgcttac gcctcctcca gcagatcctg aaaatgcagc
     2581 gtgccgcaag gctggccctg cgtgttcacc accggcagca cctcgcatcc ccgcgcgaca
     2641 aacagggaga gcgcatcgcg tagcgtcatc tcttctgcca gtgcctcacc ttctgcccgt
     2701 tcttcgcgac gcacgtaatc cgccacacta cgtaacgaaa gcaggcgcac acccagttcg
     2761 ctgcgcccaa aaaactggcg gacaaaatcg tttgccggac gagtcagcat cgtcagcgga
     2821 ttgccctgct gcaccacttc accgtgatcc atcaatacca gatgttctgc cagccgtagc
     2881 gcctcatcaa tatcatgagt gaccagcaca atggtacgcc ccagcaaacg gtgaatgcgc
     2941 gtcatctctt gttgcaacgc gccgcgcgtt accgggtcca gtgcgccaaa aggttcatcc
     3001 atcagtaaga cttgcggatc ggcagccagt gcgcgcgcca ctcccacacg ttgctgctga
     3061 ccaccggaaa gctgatgcgg ataacgctca cgtaaatttg actccagccc cagtagcgcc
     3121 attaattcgt cgatacgatc gtcaatcctc gcccgcgacc atttttgtaa ttgcggcacg
     3181 gtagcgatgt tttgcgccac gctccagtgg gggaacaggc caatagattg aatggcatag
     3241 cccatccggc ggcgcaactc cagtactggc agcgagcgaa tttcttctcc ggcaaagcgg
     3301 atcactccgc tgtcatgctc caccaggcgg ttaatcattt tcagggtggt ggatttgccg
     3361 gagccagatg tgccaatcag caccgaaaaa ctcccttcct gaaaattgag attgagatcg
     3421 ttaacggctt tttgtgcgcc gaacagtttg ctgacatggc caaattcaat cattacgttt
     3481 caccttcagc agtgcgataa gcaaatcgaa cagcgcgtcg gtaagcaccg ccagaacaat
     3541 taccgggatc acccccagca acactaaatc aatggcgctg cttagcagcc cctggaaaac
     3601 cagcgcacca aaaccgcctg cgccgattaa cgccgcaatc accgccatac ctacagtttg
     3661 caccatcacc acccgcaggc tgcgcagaaa taccggtaac gccagtggta actgaacatg
     3721 caggaatcgc tgcgccccgc tcatgcccat cgctctggcg ctctccagca catcgcgcgg
     3781 gatctggttc aaaccgacta ccacgccgcg caccagcggc agcaaggcat agagcaccag
     3841 cgcaatcagt gcgggtgtca ttccggttcc tgctatgccg agcgtcccca gccacggaaa
     3901 ggccgtaacc agcgcggcaa gcggcgcaat caacaggcca aagagcgcca ccgaaggcac
     3961 ggtctgaatg acattgagca gagaaaaaat tgccccctgc cgagcagtgg aaaagtagca
     4021 ccagatgccc aacggcacac caatcactaa cgcaggcagc accgcaccaa acagcaacgt
     4081 cagatgttgt gccagcgcgt cgtcaaacac atcctgacgg ttggcgtatt ctttcattag
     4141 tgagagatcg ttaagcgtgc cggagtacag caaccacagc ggaataatgg caatctgcat
     4201 atgcaacaac cagcgccaca gcggatgcgt ggagattcgg cggatggcat cactacaggc
     4261 cagcaatgcc agcgccgcgg ccagccagaa accactgccg aggctggtac gcgccagcgc
     4321 actgccattt tgcgccagtt gggtcgccgc ctttcctgct ccccacacca gcaatacgaa
     4381 gacgaattgc gccagaatga gtgcaaaaat gctgcctttt ttagcgggaa taaaacaggc
     4441 tgtcagccag gcgcaaccaa cgcccaccag catccagatc gtttgcggcc acagttgcca
     4501 gagatgacgc ccttcacccg agactaaacg attaggcgcg taactgataa acggcagcgc
     4561 cgctgcgatt gccgtcagca acagcagcag cgccagaaca ggattaatac gtagataagt
     4621 cacgggaaat tacttcgtcc accctttttg tttcaggtag tcggcagcca cttttttggc
     4681 atccagtcct tccactgcaa tgctggcatt cagttgctgc aatgtttttg catcgaggct
     4741 ggcgaagact ggctgtagcc actgtgccat ttgcggatac tctttcagca ccgactcacg
     4801 caccactggt gcaggcgcgt agataggttg cacgcctttc ggatcgctta aggtttgcag
     4861 ccccagcgcc gcgaccggac cgtcagtgcc gtaagccatt gcggcattaa cgccagaagt
     4921 ttgctgggca gcggctttaa tcgttaccgc cgtgtcgccg ccagccagtg acagtaactg
     4981 atcctgatcg agtttaaagt cgtaggcttt ttcaaacgcg ggtaacgcat cggcgcgttc
     5041 gataaactct gccgaggctg ccagtttgaa ggtgccgccc tctttcagat aacgactcag
     5101 gtcggcaagc gaagtgagtt tgtttttctc tgccacatcc tgacgcacgg cgatggtcca
     5161 ggtgttattc gctggtgcgg gcgtcagcca gattaacttg ttttgctctg catcgagttt
     5221 tttgactttc tcgtaacctt gctgcgcgtt tttccatgcc gcatcgtttt catctttaaa
     5281 gaagaaagcg ccattaccgg tatattccgg atagatatcc agttcaccgg aagtaatcgc
     5341 cccgcgcacc acaggagtcg ttccgagttg cactttattc accgtcggta cgccgtggct
     5401 ttccagcacc tgcaagatga tgttgccgag tagcgcgcct tcggtatcga tttttgaacc
     5461 gactttaacg ggggaagccg cctgtagcgg caggctcacg gctgccaaca taaccagtga
     5521 acctgcccag acctttgaga gtggcatgat gctttcctca ttcttttact gttgttttca
     5581 gcgaattaag agaaaagcat agttgataat gacagagtta gcctgagcgg aaggattcag
     5641 ttgcagaatc agataaatac ttaaagggca tattttgccc ggcgtttctt gccggatgcg
     5701 gcgtaaacgc cttatccggc ctacaaaggt cgaaaattca gcaagttaag atatgaacat
     5761 caggcaaagg gcgcaaacgt cgcgccctta ctaaagcatt acagcaactc aaactcgcct
     5821 ttcttaacgc gtgcggaatc agtgccgata aagacattga acttgccagg ttcggcgtca
     5881 tatttcatct gttgattcca gaacttcagc gcctcgatat caatcgggaa gctgacagtc
     5941 tgagtttcgc ccggtttcag ggtgattttc tcaaagcctt tcagctgctt cactggacgg
     6001 ctcatggaag ccgtcacatc ctgcaagtac atctgtacta ccgtggcccc ttcgcgttta
     6061 ccggtgttcg tcacctgcac gctggcggtc actttgccgt cacgcttcat ggtcggcgca
     6121 gaaagtttca catcagagac ggtgaaagtg gtatagctca gaccatagcc gaacggataa
     6181 agcgccccgt tagcttcatc aaaataacgc gaagtgtatt tgttcggctt gtcggcatta
     6241 tacggacgac cggtattcag atggctgtag tacaccggga tctgcccgac agaacgcggg
     6301 aaggacattg gcagcttgcc ggacgggttg taatcgccaa acaatacatc ggcaattgca
     6361 ttaccgcctt cagtccccgc aaaccaggtt tccagaatcg catcagcctg ctgatcttct
     6421 ttcaccagcg ccagcggacg cccgttcatc agcaccagca ccagcggttt accggtggct
     6481 ttcagcgccg taatcaagtc acgttggctt tgcggaatag tgatatcggt acggctggag
     6541 gcctcgtgcg ccatcccctg tgcttcaccg accacagcca ccaccacatc ag
//
LOCUS       DAJVRF010000181         6473 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJVRF010000181 DAJVRF010000000
VERSION     DAJVRF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6473
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.181"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            7..1710
                     /locus_tag="OQN13_003621"
     CDS             7..1710
                     /locus_tag="OQN13_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001475214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCS1616882.1"
                     /translation="MDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVLARWD
                     TPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQLALGNYRLTVRAVNAWGQ
                     QGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIADIRQ
                     VETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQPSDDASGYLD
                     FFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSNEITQTVNKKLEDQSAAIQ
                     QIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPDGMQSQVLLAADRIAM
                     VNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNPSAFSLTPDGKLTAKNADI
                     SGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVSKSFPKQAGTWGNTETPNG
                     TVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGTRYTGYGFEVRKNGVLIAS
                     RETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISI
                     R"
     gene            1781..2297
                     /locus_tag="OQN13_003622"
                     /pseudo
     CDS             1781..2297
                     /locus_tag="OQN13_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            2362..3766
                     /locus_tag="OQN13_003623"
                     /pseudo
     CDS             2362..3766
                     /locus_tag="OQN13_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            3715..3867
                     /locus_tag="OQN13_003624"
                     /pseudo
     CDS             3715..3867
                     /locus_tag="OQN13_003624"
                     /inference="COORDINATES: protein motif:HMM:NF015371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            3844..4023
                     /locus_tag="OQN13_003625"
                     /pseudo
     CDS             3844..4023
                     /locus_tag="OQN13_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            4738..5445
                     /locus_tag="OQN13_003626"
                     /pseudo
     CDS             4738..5445
                     /locus_tag="OQN13_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            5445..6026
                     /locus_tag="OQN13_003627"
     CDS             5445..6026
                     /locus_tag="OQN13_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1616883.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPVAPIG"
BASE COUNT         1734 a         1576 c         1806 g         1357 t
ORIGIN      
        1 gccatcgtgg ataacggggc gcactttgac ggcgaccaga gcggcacggt gaatggtgtc
       61 acgccgccag cggtgcagca cctgactgcc gaagtcaccg cagacagcgg ggaatatcag
      121 gtgctggcgc gctgggacac gccgaaggtg gtgaagggcg tgagcttcct gctccgtctg
      181 accgtaacag cggatgacgg cagtgagcgg ctggtcagca cggcccggac gacggaaacc
      241 acttaccgct tcacacaact ggcgctgggg aactacaggc tgacagtccg ggcagtaaat
      301 gcgtgggggc agcagggcga tccggcgtcg gtatcgttcc ggattgccgc accggcagcg
      361 ctgtcgcgga ttgagctgac gccgggctat tttcagataa ccgccacgcc gcatcttgcg
      421 gtttatgatc cgacggtaca gtttgagttc tggttctcgg aaaagcggat tgcggatatc
      481 aggcaggttg aaaccacagc acgctatctt ggcacggcgc tgtactggat agccgccagt
      541 atcaatatca aaccgggcca tgattattac ttttatatcc gcagtgtgaa caccgttggc
      601 aaatcggcat tcgtggaggc tgttggtcag ccgagtgatg atgcatccgg ttatctggat
      661 tttttcaaag gcgagatagg gaaaacccat ctggctcagg agctgtggac gcagattgat
      721 aacggtcagc ttgcgcctga cctggctgaa atcaggacgt ccattacgga tgtcagcaat
      781 gaaatcacgc agaccgtcaa taagaaactg gaagaccaga gtgcggcaat tcagcagata
      841 cagaaggttc aggttgatac aaataataac ctgaacagca tgtgggctgt gaagctgcag
      901 cagatgcagg acggacgcct ttatatcgcg ggtattggtg ccggtattga gaacacccct
      961 gacggcatgc agagtcaggt gctgctggcg gcggacagga ttgcgatggt taatcctgcg
     1021 aatggcaaca caaaaccgat gtttgttggt cagggcgatc agatattcat gaacgacgtg
     1081 ttcctgaaac gcctgacggc ccccaccatt accagcggtg gaaatccatc ggcattttcc
     1141 ctgactccgg acggaaagct gaccgctaaa aatgcggata tcagtggcag tgtgaatgcg
     1201 aacgccggga cgctcaacaa tgtcacggta aatgaaaact gtacgattaa gggcatgctg
     1261 gaggcgactc aggtcagagg tgacttcgtt aaagctgtat ccaaatcatt tccgaaacag
     1321 gctggtacgt ggggtaacac ggaaacacca aacgggacgg ttacagtcac cataagcgat
     1381 gatcataact ttgaccgcca gattattatt ccgcccatta tttttaacgg tatagcgtat
     1441 gacgatccgg ggagcggaaa taacccagga ggcacgcgat acacgggtta tggttttgaa
     1501 gttcgcaaaa acggcgtatt aatcgcatcc agagaaacta aaggtgccat tcccggtagt
     1561 tacagtgcag ttattgatat gcctagtggt ggtggtagcg tcactctgga gtttaagatt
     1621 ttccagaaag gcaatcaggg ggcaggcaat atcaccgact gtacggtgat tgtgaccaaa
     1681 aaagcggctt ccggcatcag tattcgttga aatatttata accccaataa atggcgtcag
     1741 gaatgacgcc ttttttattg cagaaaagcg agaggtaatt atgcgtaaag tttgtgcagc
     1801 cattttgtcc gcagccattt gcctggccgt atccggtgcg cctgcatggg cgtctgaaca
     1861 tcagtccacg ctgagcgcgg ggtatcttca tgcccggacc aacgttcccg gcagtgatga
     1921 tctgaacggg attaacgtga aataccgtta tgagtttacg gatacgctgg ggctggtgac
     1981 gtcattcagc tatgcaggag acaagaatcg ccagctgacc cgttacagcg atacccgctg
     2041 gcatgaagat tccgtgcgta accgctggtt cagcgtgatg gcggggccgt ctgtgcgcgt
     2101 gaatgaatgg ttcagcgcgt atgcgatggc gggtgtggct tacagccgtg tgtcgacttt
     2161 ttccggggat tatatccgcg taactgacaa taaggggaaa acgcacgatg tgctgaccgg
     2221 aagtgatgac ggtcgccaca gcaacacgtc tctggcgcac tgacggtttc atcgtgggtg
     2281 tcggttataa attctgatta gccaggtaac acagtgttat gacagcccgc cggttcaggc
     2341 gggctttttt gtggggtgaa tatggcagta aagatttcag gtgtactgaa agacggcaca
     2401 ggaaaaccgg tacagaactg cacaatccag ctgaaagcaa aacgtaacag caccacggtg
     2461 gtggtgaaca cgctggcctc agaaaatccg gatgaagccg ggcgttacag catggacgtt
     2521 gagtacggtc agtacagcgt tattctgttg gtggaaggat tcccgccgtc acatgccggg
     2581 accatcaccg tgtatgaaga ttcccgaccc ggtacgctga atgattttct cggtgccatg
     2641 acggaggatg atgcccgtcc ggaggcactg cgccgttttg agctgatggt ggaagaggtg
     2701 gcgcgtaacg cgtccgcggt ggcacagaac acggcagccg cgaagaaatc agccagcgat
     2761 gccagcacat cagcccgtga ggcggcaacc catgcgactg atgctgcaga ttcagcacgt
     2821 gcagccagca cgtcagccgg acaggccgct tcgtcggctc agtcagcgtc ttccagcgca
     2881 ggaacggcat caacaaaggc cactgaagcc tcaaaaagtg ctgccgctgc agagtcctca
     2941 aaaagcgcgg cggctaccag tgccggtgcg gcgaaaacgt tagaaacgaa tgcggcagcg
     3001 tcacaacaat cagcagccac ttctgcatcc accgcgacca cgaaagcgtc agaagctgcc
     3061 acctcagccc gggatgcgtc ggcttcaaaa gaggcggcaa aatcatcaga aacgaacgca
     3121 tcatcaagcg ccagtagcgc agcttcctcg gcgacggcgg ctgcaaattc tgcgaaggcg
     3181 gccaaaacgt ccgagacgaa cgccaggtct tctgaaacgg cagcgggaca gagtgcctca
     3241 gctgcggcag gctcaaaaac agcggctgcg tcgtctgcca gcgccgcgtc aacaagtgcc
     3301 gggcaggcct cagccagtgc caccgccgcc ggaaaatcgg cagaaagcgc cgcatcatcc
     3361 gcttcaacag ccacaacgaa ggctggcgaa gccactgagc aggccacggc agcagcgagg
     3421 tcagcttccg cagcgaagac atccgaaacg aacgcgaaag cgtcggaaac ccgtgcagaa
     3481 tcctcaaaaa cggctgccgc atcgtccgcc agttcggcgg cgtcatcggc atcatcggcg
     3541 tctgcttcaa aagatgaggc gaccagacag gcgtcagcag cgaagggcag cgccacgacg
     3601 gcatccacga aggcgacaga ggctgctggt agtgcgacgg cagcagctca gagcaaaagt
     3661 acggcggaat ctgcagcaac gcgcgctgag acagcggcaa aacgggcaga ggatattgca
     3721 tccgccgtgg cgcttgagga tgcgagcacg acgaaaaagg ggatagtaca gctcagtagt
     3781 gcgactaaca gcacttccga gtcactggcg gcaacaccaa aagccgttaa ggccgcgtat
     3841 gacctggcta acgggaaata caccgcacag gatgcaacga cagcacagaa agggataatc
     3901 cagctaagca gcgcgaccaa cagcacgtct gaaacgctgg cggcaacgcc aaaggcagta
     3961 aaagcagcca atgacaatgc tgagaaacgt ctgcagaaag atcagaacgg tgcggatatc
     4021 cctggcaaag acacctttac gaaaaatatt ggtgcctgcc gtgccttcgg tgggtcagta
     4081 agcacaacaa caggaaactg gacgactgca cagtttatcg agtggctgga ttctcaggga
     4141 gcatttaacc atccatactg gatgtgcaag ggttcctggt cttatggcaa taataaaatc
     4201 attactgata ctggctgcgg taatattcat ctcgccggag ctgtcattga agtaatgggg
     4261 ataaagtcag cgatgacgat ccgcattacc actccgacca catccagcgg tggcggaaca
     4321 accaacgccc agtttaccta tattaatcac ggaacagatt attcacctgg ctggcgaagg
     4381 gactataact ccagaaataa gccaacggca tcagagatcg gggcgctacc gtcaggtgga
     4441 acagcagtat catcagttaa tctggcttca aaaggtcggg tagccgcgct gacagacaat
     4501 acgcaggggg cagcaggtct tgagttatac gaggtgtata acaacggata tccaacagcg
     4561 tatggaaata tcattcacct gaaagggatg acagccgttg gcgaaggtga gttactcatc
     4621 ggctggagtg gtacaagcgg tgctcatgct ccggcattta ttcgttcacg acgggatacg
     4681 accgacgcaa actggtcgcc gtgggcgcag ctttacacct cggctcatcc tcctgcagag
     4741 ttttatccag tcggtgcacc aatcccgtgg ccatcagata ccgttccgtc tggttatgcc
     4801 ctgatgcagg ggcagacttt tgacaaatct gcttacccga aacttgcagc cgcttatccg
     4861 tcaggcgtga tccctgatat gcgtggctgg acgattaagg gcaagcccgc cagtggtcgt
     4921 gccgtattgt ctcaggaaca ggatggcatt aaatcgcaca cccacagcgc cagcgcatcc
     4981 agtacggatt tggggacgaa aaccacatcg tcgtttgatt acggcactaa atccacgaat
     5041 aacaccgggg cgcatacgca cagtctgagc ggctctacaa gttcagctgg ggcccaccaa
     5101 catagtcaga ctgggcccag aacgaatagc gggagtcaac ctactggaat gtttccggca
     5161 gggagtactc aagttagcgg gactaatcag gttggtatca gtggctcatt aaccagtggc
     5221 acctcccaat gggtaggtaa atcatcttca gaaggaaacc atacacactc actgtccggc
     5281 acagcagcat ctgcgggtgc acacgcgcat actgtcggta ttggtgctca tacgcactct
     5341 gttgcgattg gttcacatgg acacaccatc accgttaacg ctgctggtaa cgcggaaaac
     5401 accgtcaaaa acatcgcatt taactatatt gtgaggctcg cataatggca ttcagaatga
     5461 gtgaacaagc acggaccata aaaatttata acctgctggc cggaactaat gaatttattg
     5521 gtgaaggtga cgcatatatt ccgcctcata caggtctgcc tgcaaacagt accgatattg
     5581 caccgccaga tattccggct ggtttcgtgg ctgttttcaa cagtgatgag tcatcgtggc
     5641 atctcgttga agatcatcgg ggtaaaacgg tttatgacgt ggcttccggc gacgcgttat
     5701 ttatttctga actcggctca ttaccggaaa atgtcacctg gttatccccg gaaggggagt
     5761 ttcagaagtg gaacggcaca gcctgggtga aggatacgga agcagaaaaa ctgttccgga
     5821 tccgggaggc ggaagaaaca aaaaacagcc tgatgcaggt agccagtgag catatagcgc
     5881 cgcttcagga tgctgtggat ctggagatcg caacggagaa agaaaccttg ttgctggaag
     5941 cttggaaaaa gtatcgggtg ttgctgaacc gtgttgatac atcaactgca ccggatattg
     6001 aatggccagt agcacctata gggtaaattc gtaatgatta cctaaatacg ttattctttt
     6061 gttaaaaaag tgattttgct ataggtaggg agtatgttct tggattaaga ataaaaaatc
     6121 gccatgaaag agcttcatgg cgagtacata aacgtacaaa atgtgatgaa agtagagtgg
     6181 cgtaagccac tatattaaga tatgacaata gcgaagaaaa gtaaataaca ttttatggac
     6241 gtttaattgt taattataaa aactagagtg cccgatattg tttatcttgc tttaaaataa
     6301 tgtactcaaa ccaaattttt gagctaaaag ctcaaataat ataaatgcaa taataatgtt
     6361 taataagaca attagcttgc tttgtatttt catttcggaa tactgttaag taactttgat
     6421 gaccctaaca atagatcgga attttcttag agtcaatggt cactgttttt gct
//
LOCUS       DAJVRF010000182         6447 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJVRF010000182 DAJVRF010000000
VERSION     DAJVRF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.182"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..187
                     /locus_tag="OQN13_003628"
     CDS             <1..187
                     /locus_tag="OQN13_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616884.1"
                     /translation="LFGASLIMVVGCSSKENALCHPQPKPPAPPAWAMMPPSNSLQLL
                     DETFSVSGTESSATRQH"
     gene            complement(320..832)
                     /locus_tag="OQN13_003629"
     CDS             complement(320..832)
                     /locus_tag="OQN13_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCS1616885.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1027..1791
                     /locus_tag="OQN13_003630"
     CDS             1027..1791
                     /locus_tag="OQN13_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616886.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1742..3145
                     /locus_tag="OQN13_003631"
     CDS             1742..3145
                     /locus_tag="OQN13_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCS1616887.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            3145..4611
                     /locus_tag="OQN13_003632"
                     /pseudo
     CDS             3145..4611
                     /locus_tag="OQN13_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4502..5245
                     /locus_tag="OQN13_003633"
     CDS             4502..5245
                     /locus_tag="OQN13_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCS1616888.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5249..>6447
                     /locus_tag="OQN13_003634"
     CDS             5249..>6447
                     /locus_tag="OQN13_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCS1616889.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAE"
BASE COUNT         1718 a         1539 c         1760 g         1430 t
ORIGIN      
        1 gctcttcggc gcaagcttga taatggtggt cgggtgctcg tcaaaggaaa atgccctgtg
       61 ccatcctcag ccgaaacctc cagcgcctcc ggcatgggca atgatgccac cgtcgaactc
      121 tctccagttg ctggacgaaa cgttctcggt atccgggacg gaatcatcag cgaccagaca
      181 gcattgagaa tgcttcagga gtacatcagg atccaatgcc ttggggggta gcggtaattt
      241 tactcattat ccttcaaatc agattctgtt gtcagaggaa tgggggaagc tggattcgca
      301 agagcttttt atgggcagtt tagctaaaaa aaggcaacac gaagctaaaa ctaccaacat
      361 aaagtgctga ccagctagca gctgaaatca gactcactgt atacactttc actggatgaa
      421 gttgcaacat tcctgccacc aaaggagcaa tataacgcag cacggcgata aagcgcgaag
      481 tgaaaagtat tgacacagag ttattttgca actgaaggcg aacccgttct attgtattgg
      541 aacggcttga tattatacat gcaagttgag ggatatgccc aattattata ccaagatggt
      601 aattaacgat agtaccgcac catgcgccac tcatgaccgt gattccagct tcccatggtg
      661 aaagagtcgt cttgctgact gtaattacag tggccatcat aattgatgct ggtggcaata
      721 tcgccgaaat gagtaaagtg gattttgtga acgctattat aaaaagaagc ccccataggc
      781 gatatggatg aagtacaaaa tagttcatca aagcatttat ccactccatc aatgtctttc
      841 cttctgtgaa tttttctttg ctatttaacc gtgatacgac ttaatttagg cttaaacacc
      901 ttacatttac aaggtactcc tgtggggggc cctgccacgg agcgtcgcag gagtgagtga
      961 tgaggaagaa ttaaagcaac ctattgtgac aggcagcgat aacagtaaat cacaggaagc
     1021 ataatcatgg caaaaccgga ctgggaggcc atcgaatcgg cataccgggc cggagtcctt
     1081 agcctccgtg atataggcga gaaatacggc gttacagaag gggctatcag gaagagggcc
     1141 aaaaagcttg gttgggcacg cagtggcggt acgcaggttt gcaaaaatgg tacgcaaaaa
     1201 aggaaagtgc gtaccagcag aaagcctgcc attactggcc ttacacaaaa aagtacgcaa
     1261 ctaaaaacag aatctacacc ggatacgaaa ccgatacgcg gaatgcgtac cgatcccccg
     1321 actaacccat tccaacccgg taaccagcag gcattaaaac acggtggtta tgcccgtcgc
     1381 cttctgctca aagatgaggt gatagaggac gctaaagcgt tgacgctcga ggacgaatta
     1441 tttcgccttc gtgctaacaa ccttgtcgct gcagagaata ttggtcggtg gttggtgtcg
     1501 ctggaagatg ctaatgggga ccaggaaagg aagatgctga ttgaaaatat cagcgccgcc
     1561 gagaaagcaa tgatgcgcaa tacagttcgt attgagtcca tcgttggcac gcttgcgacg
     1621 gtaggaaaaa tatttgctga tacagcctac cgcaaggccg ccactgataa ggtgtctctg
     1681 gaggctgatc gtcttcgccg tgatgcaggt attgatgatg gcaatggaga gcgtgacctc
     1741 aatgacttct actctgacat ccaaaccgac gctgaatccg gctttacgta gtttctggac
     1801 tatgcgggca cgtaacaaag tgctttatgg tggtcggtca tcgtcaaaat catgggatgc
     1861 cgctggcatt gccatatttc tgtcgaataa atacaccctg cgtttttgtt gtgcccgtca
     1921 gatccagaat aaaatcgaag agtcggtgta taccctgctc aaaattcaga tagacaggtt
     1981 tggtctgcgg caccgtttcc gtattctgaa caacaaaatc attaaccggg ttactggctc
     2041 ggaatttgtt ttttatggat tatggcgcaa catcgaagaa attaagtcac tggaggggat
     2101 cgatgtgttg tggctggaag aagcccacgc actgacggaa taccagtgga aaattctgga
     2161 gccaacgatc cgtaaagagg gttcggaatg ctggttcata ttcaaccccg gacttgttac
     2221 tgatttcgtc tggcgcaact tcgttgttga tccgcccgaa ggcactctca tccgcaaaat
     2281 taactatgac gaaaatccgt ttctgtctga caccatgctt aaggttatcg acgcggcgcg
     2341 acgccgtgat ccggatggtt ttaaacatgt gtatgagggc gttccggagt ctgatgatga
     2401 tgcggcaatc atcaaactgt cttggataga agccgcagtg gatgcgcaca aaacgttaaa
     2461 tttcgaaccc agtggaagaa agcgtattgg ctttgacgtg gctgacagtg gtacagataa
     2521 gtgcgctaac gtttaccgtc acggatccgt tgttttctgg gccgacgaat ggaaggccaa
     2581 agaagatgaa ttactgaaga gctgccagcg tacttatcag gcggcgctgg agcgtgaagc
     2641 agatattgtt tacgactcta tcggtgttgg tgcgtctgcc ggtgctaaat tctctgaaat
     2701 taacgctgac cggaagagcg agaacgcata cgcgcgacgt gtgaattacc agaggtttaa
     2761 cgccggtgct ggtgtgcatg agccagatga cgagtacaac ggcatcccca acaaagactt
     2821 tttcgcaaat cttaaggctc aggcatggtg gctggtggct gaccgtttca gaaatacgtt
     2881 taacgccatt aacaacggag aacagtatcc tgtggatgag ctgatcagca tagattctcg
     2941 ttgtccgttg cttgaaaagc tgaaactgga actgacaaca cctcatcgtg atttcgaccg
     3001 taacggacgt gtgatggtcg aaagtaaaaa agacctcgca aaacgcgaga taccgtcacc
     3061 aaacgttgct gatgcattca ttatggcctt cgcgccaatc gatacatcgc tggatatctg
     3121 ggaacagctg gggagacagg cctgatggca cgaaacaaac aagccctgcg gcgaactgtg
     3181 caggccacag ctgatggtta tgagaatttt attgcccgcg tagggatgca gacacctaac
     3241 cagcactcag catccaccta ccgggctaat ttcaccagtc gtaaccgcat gctggtggaa
     3301 tggtcctatc gttcatccta gatcatcggc gaagcagtcg atgctatccc ggatgatatg
     3361 acccgcaaag gcattcgcat cacttcggaa attgatgcaa aagatcgtgg cattctcgaa
     3421 tcacaactgg atgagttgca aatctgggat gcgctgaatg acgtgctgaa atggtcgcgc
     3481 ctctacggcg gcgcggtggg tttcatcatg attgaggggt aggcaccaat gaccccgctg
     3541 cgacccgaaa ccatcggtaa gggcaagttt aaggggattc tcccgctcga ccgctggatg
     3601 gtcgacccgg cactgacccg ccgcattaaa gatatggggc cggacctggg taaacctgag
     3661 ttttacgatg tggtgaccac agcaacggga attcctgcct ggcgcattca tcacagtcgc
     3721 ctgattcgct ttgatggcgt cacgttgcca tttcagcaga agatgaccga gaacgaatgg
     3781 ggaatgtcgg ttgtagagcg tatctgggat cgtcttaccg cgttcgacag cgctactgtc
     3841 ggcgcggcgc agctggtcta caaggcgcat ttgcgtacct acagcgtgga gaagctacgc
     3901 gagcttatcg cacttggtgg tcctgcgtat gaagcgttgc tgaagaatat cgacctgatt
     3961 cgacagttcc agagtaatga aggcatgacg ctcatggact cgcgggataa gtttgaaacc
     4021 catcagtaca gcttcagtgg tctggatgac atcctttcgc agtttgtaga acagattagt
     4081 ggcgctgttg gtatcccact ggtgcggttg ttcggacagt ccccgaaagg attttctacc
     4141 ggtgatgcag accttgccaa ctattacgat cgcatcagtt cgttgcagga gaggcgttta
     4201 cgtcttccgg tgcggcggat actggacatc atgcatcgtt cggaacttgg caagccgctg
     4261 ccggacgatt tcacgtttga gtttaacccg ctctggcaaa tgtctgatgt cgatcgctca
     4321 acggtggcgt taaacaccac caacgcaatc agtacggcgc tgggtgatgg tctgatgaca
     4381 ctgaaagccg ctatgactga tttgcgagaa aattctgacg taaccggcat cggggcatcc
     4441 attaccgacg aggacatcga gaatgccgaa gatgaagcgc cgcccggcat cggcgaacct
     4501 gatgacgaac cgcaggagcc gtcaggcgga aatccggtat cgaaccagcc tacgcaggat
     4561 agcgagggcg gtcggagaca tcgtaaatgg tcgctacgat ggttcaaatg acagtatcac
     4621 ggaaattatt gcggcgctgg agcgttacag tgaaatcatc accccctggg cgacaaaggt
     4681 cgcggaaaac tttactgcgg acctaacccg gcagaacgag aaagtttggc ggcaacacag
     4741 caagaacatc agtcgcgagc tccgcaatct tgtggaaagc gctcctgtgg gccaggtgat
     4801 gcaatccatc atcgccgaac aggtcaagta catcaaatct ctgcctctcg aggccgcaga
     4861 cagggtgtac gacatccaga acaaagcgat agaggctgtt gtcaccggtg gtcgggcgga
     4921 gcagtttgct aaagagattg catctaccgg cgatgttgct aagtctaggg ccgatctgat
     4981 tgcccgaacg gaactgggaa gagcaacggg cgcgctggat atggcccgag cgatagctat
     5041 tggctcggat ggttatattt ggcgtacagc cgatgatggc gatgtccgag attcccacga
     5101 tcacatgaaa ggtaaattcg tccgctggga ttcacctcca actttggacg gcatgaccgg
     5161 ccacgcgggc gagctgccaa actgccgctg ctataaagag atcgtgtttg ttcgcgttcc
     5221 attcgcaatg aaaagggcag cataacccat gaaatacttt tttgagacca ggctcgggga
     5281 aacccgatac cgcctggctg acggctcgtt gctgtgcaaa gacgtgccga taggacgaac
     5341 aggtaagcag ctctatggtg ctgatgacct gccaaaactg aaacccgata agttcggtga
     5401 aatagtcgtc acgcgttctc ctgagcaggt attccatccg gccacgcttg cctcattcga
     5461 agggatgagc atcacgatcc tgcatcctga agatgaaaac gggaatgtgc ggctggtaaa
     5521 tcccgagaac tggaaagagc ttgctgtcgg gcacctccag aatgtccggc gcgggacggg
     5581 tgagcagtct gatttgatgc tggctgacct tatcgtcaaa gacgaaagcg ccattcagct
     5641 tatcgaagat ggcctgcgcg aagtgtcgtg cggctatgac gcggagtacg agcagaccga
     5701 gccaggtaaa gccgagcagg tcgatattac cggaaaccat gtggctcttg tccctaaagg
     5761 cagagccgga aatcgttgtg caattggaga cagagacaca atggcaaatc aaaagaaaaa
     5821 ctggtggaac cgcatgcgtg cggccatcaa gacaggagat gccgacacca tgaacgaact
     5881 ggtggagtcg gctcccgcat cggttacagg agatgagggg gatttgccgc agggcgttaa
     5941 tctcaacatc aacctgtccc cgcagcaacc actaccggac aaagcaccag agatgggcgg
     6001 agatccaacc ggcgacagtg atgatgacct caaaacatta ctgaaagccc tgctggctaa
     6061 gctggaaaga aatgccacgg gcgataacga taataagcct gacgataatc cgaccggtga
     6121 cggcgaggac gatgaagagg aaaccacgat tactggtgac tcagcctggc gtgccgaagt
     6181 tatcgttccg ggtatcgatc tgagccgtaa gatgaaaccg accgcgttca aacgcgaggt
     6241 tctggcttct gctgacaaaa cgctggttcg ccagatagtc ggtgatgcgg atatccgcaa
     6301 attaccgaaa caatcggtcg acatggcgtt taatgccgtg tctgaggttg ccaaagggcg
     6361 aaacacccgc gccaccaccg gcgatgcaca gcgcctaaac atgggcatga ccagtatcgc
     6421 ttccctgaac aaacaaaacg ctgaatt
//
LOCUS       DAJVRF010000183         6327 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJVRF010000183 DAJVRF010000000
VERSION     DAJVRF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.183"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..356
                     /gene="mcbR"
                     /locus_tag="OQN13_003635"
     CDS             <1..356
                     /gene="mcbR"
                     /locus_tag="OQN13_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCS1616890.1"
                     /translation="TPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNM
                     PILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAE
                     IMQQNIAILYQQYNR"
     gene            complement(392..2494)
                     /locus_tag="OQN13_003636"
     CDS             complement(392..2494)
                     /locus_tag="OQN13_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCS1616891.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2736..3797
                     /locus_tag="OQN13_003637"
     CDS             2736..3797
                     /locus_tag="OQN13_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCS1616892.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3912..5411)
                     /gene="ansP"
                     /locus_tag="OQN13_003638"
     CDS             complement(3912..5411)
                     /gene="ansP"
                     /locus_tag="OQN13_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCS1616893.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5678..6295
                     /locus_tag="OQN13_003639"
     CDS             5678..6295
                     /locus_tag="OQN13_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HCS1616894.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGSGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
BASE COUNT         1704 a         1594 c         1513 g         1516 t
ORIGIN      
        1 tcaccccgca agacctgacg gaactccagg aattgctcga gaaattacag caggcgcaag
       61 aaaagggcga catggaacaa atcattaatg taaacaggct atttcgctta gcgatttatc
      121 atcgctcaaa tatgcccatc ctgtgtgaga tgattgagca actgtgggtc aggatggggc
      181 ctggtttaca ttatctctat gaagcgatta atccagcgga attacgggag catatagaaa
      241 actatcatct gttactcgct gcgttaaaag caaaagacaa agagggatgc agacattgtc
      301 ttgctgaaat tatgcaacaa aatattgcta ttttatacca gcaatacaat cgttaaagta
      361 aatgcccggc aaataccggg catcaaggta attactcaaa tctccacgca atattcatgc
      421 cgacaccata atttcgtccg ggcgaaggtt cgtaatatcg cccgtttgac tcattgacaa
      481 tgacagaacc aacgtattct ttatcgaata aattatcgac acgaccaaat aaatccacag
      541 ttaaattgtg gtaattgtat ttatacccgg tgaataagcc gacgagagta taagacggcg
      601 cttttgccgt attttcatca tctgccataa tatcgcccat ataacgcgct tccgtgcctg
      661 cataccaacc atcttccggt acataaccta tcgacgcaaa gcccatatta cgggcgatcc
      721 ctggcatccg attaccgtta caatcctgtt cattgcaaac attgctgcga taggtcgcat
      781 caagccaggt ccaggacgcg tttacgcgaa aatctcctgc gaaacgttga tcccatgcca
      841 gttcagcgcc ttgacgacgg gtctttccgg catttttgta agtcgtacgc ccaccgctac
      901 tgctatcgac aacaatttca tcatcagtgt cggtctgaaa caatgcgaga ctaagcagcc
      961 catcaccaat acgcgtttta ctgccgatct caattgtatc gttggtggat ggttttaaac
     1021 caaagttcat accgctttgc ccatcagcac gataagacag ctcattaatc gtcggcgttt
     1081 caaaacctcg cccggctgcc agatagatat tccaggcatc ggtcattgca tattttaacg
     1141 aaccggcagg tagccattta tgataactgg catcaccgct gtcatcgccg ttacccggag
     1201 taacgtaatg gtcgttggaa tcaaaccaca cggagctgta gcgcacgcca gcatccagcg
     1261 acagtttttc gctcagctgc cactgcgtct gtaaataggg atcgatgttc cacatcagat
     1321 tgcgttcgtc gcgacgcaac tcaccttttt gcccgtactc cggcatgccg ctattcaggc
     1381 ggaagttatt gtagcccttg cggttttcac tcatgttttc gtagttcagg ccggtagtga
     1441 acgtgaccgg aacgcccagt tcaccacggt gtgtccagcg gctgtctatc ccctggtaat
     1501 ggcgttgcag ggtaatcacg ccgcccgcat gtgacgggtt aagttgtggt gccatgggta
     1561 ttgactggta ctgggtcgtt tctcgctctc cggcatacat catcacactc atatcatctt
     1621 gcgcgctcag gctacgctca tagcgcaacc cagcctgagt ttgcttgatg gtttttcgcg
     1681 tgtcgtactg ttctgcacga ggcgcttgtt gtggattagc cttccattct gctttggtta
     1741 gcccacccgg gtcatctgct ttgatatcca cactattgaa aatcagactt aatttgctgg
     1801 cttcatcaat gcgtacgccc agtttggcat tggctaaatt tttctgtgcg ccactatggt
     1861 cacgatagcc gtgggtcgta aaacgcgtgg ttgagacggt gtaatcgaca tcgccaggct
     1921 gtgtgccgtc tcccgttgcg cccgttgctt tcagcccata gcgccagctg ccaaaactgc
     1981 cgtagtaact actggcttca atggttggtg gctgttgtcc ggtctgggtg gtgacattca
     2041 ttaccccacc agacgcgttg ccatacaggg cagagaaggg gccacgcagc acttccacat
     2101 tttgcacact gcttaaatcg atgttggatg tttgcccttg cccgtcgggc atggtggcgg
     2161 gaataccgtc cacatacagg cgaataccgc gaataccgta agtggagcgg gagccaaatc
     2221 cgcgaatcga cagctgtaaa tcttgcgcat agttctgccg gttttgtacc tgcaaaccag
     2281 gcacgtcggt cagtgattcg gacaagttaa tgcgcggtgt tgccaggcgc atctcctcgc
     2341 catccaccac gcttactgct gctggggtat ccagttctga aaccacctgc ggtgcggcac
     2401 tgacaatcat ggtctgttca tcagcggcaa aaacaacggg ggaaagggca agtagtgcgg
     2461 gcaaaacggt ctgtcggacg gaaaaaatct tcatgaaaaa agccaggtta agaatgggaa
     2521 aacgccgtta tggtaataaa attgtaaatt catggaaaac gaaacggcac gatacgttaa
     2581 gtaattgaga aaattgtagt cgtaacggca agaaatgctc cacatttgag aaaataatga
     2641 ttaccattcc catttataac aagagcgtaa cgatgattac gcttagcgaa gcattgtgaa
     2701 gcagcaaaaa tatcggttca tcaaagggag tcgtcatgca tttacgtcat ctgttttcat
     2761 cgcgcctgcg tggttcatta ctgttaggtt cattgcttgt tgcttcatca ttcagtacgc
     2821 aggccgcaga agaaatgctg cgtaaagcgg taggtaaagg tgcctacgaa atggcttata
     2881 gccagcaaga aaacgcgctg tggctcgcca cttcgcaaag ccgcaaactg gataaaggcg
     2941 gcgtggttta tcgtcttgat ccggtcactc tggaagtgac gcaggcgatc cataacgatc
     3001 tcaagccgtt tggtgtcacc atcaataaca cgactcagac actgtggttt ggtaacactg
     3061 taaacagcgc ggtcacggcg atagatgcca aaacgggcga ggtgaaaggc cgtctggtgc
     3121 tggatgatcg taagcgtacg gaagaggtgc gcccgctgca accgcgtgag ctggtagctg
     3181 acgatgccac gaacaccgtt tacatcagtg gtattggtaa agagagcgtg atttgggtcg
     3241 ttgatggcga gaatatcaaa ctgaaaaccg ccatccagaa caccggtaaa atgagtaccg
     3301 gtctggcgct tgatagcaaa ggcaaacgtc tttacaccac taacgctgac ggcgaattga
     3361 ttaccatcga caccgccgac aataaaatcc tcagccgtaa aaagctgctg gatgacggca
     3421 aagagcactt ctttatcaac attagccttg ataccgctag gcagcgtgca tttatcaccg
     3481 attctaaagc cgcagaagtg ttagtggtcg atacccgtaa tggcaatatt ctggcgaagg
     3541 ttgcagcacc ggaatcactg gctgtgctgt ttaacccagc gcgtaatgaa gcctacgtaa
     3601 cgcatcgtca ggcaggtaaa gtcagtgtga ttgacgcgaa aagctataaa gtagtgaaaa
     3661 cgttcgatac gccgactcat ccaaacagcc tggcgctgtc tgccgatggc aaaacgctgt
     3721 atgtcagtgt gaaacaaaaa tccactaaac agcaggaagc tacccagcca gacgatgtga
     3781 ttcgtattgc gctgtaatag atatggcgag ggtgaaaacg gagagacttg cctgactttt
     3841 atcacgccgc atgcggcaca ccttcgtcag taatctaaat ttgcccggca cgtagccggg
     3901 caattttgca attagctcgc cgtttcaggc ttaaacacaa tttcctgttt ttcttcatct
     3961 tcctcgacga ctggcgcagt gctgtgaatt tcagcaacgc gtttgcgcac accaaaccag
     4021 cctataacca gcagaatacc gataatcggc agcgccgcga tagtataagt cccgttcggg
     4081 taatcgaacg ccatcagcac aaggacactc agtaaaaaca gtaatgtcag ccaggaagtg
     4141 aagggcgcgc caggcagttt aaaactgaca tccgctgctt tgccttcttt aatcgcttta
     4201 cgcaggcgca tctggcacac gatgataaac gcccatgaag cgatgattcc cagcgacgcg
     4261 aagttcaaca caatctcaaa tacgcgcgac ggcaccagat agttgaggaa tacgccgacg
     4321 acatacacaa ccagtgtcgc cagaatcccg gcatacggca catgctgacg actcattttc
     4381 gccataaaac tcggtgcgga accgcccatc gccatcgagc gcagaatacg cccggtgcag
     4441 tacagacctg agttcaggct ggagagggca gcggtcagca ccacaatgtt cataatgctg
     4501 ccgatatatg gcacacccag tttagagaaa aacgtcacga acggactttg ccccgcctga
     4561 tacgcgctcc acggcaataa cataaccagc aacaccacgg agccgacgta aaacaggcca
     4621 atacgccaaa tcacactgtt aatggcttta ggcaccatgg tctgcggatc tttacattct
     4681 ccggcagctg tacccaccat ttcaatggag gcaaaagcaa acactacgcc ctgaatcaac
     4741 accagcgcag gcagcaaacc gtgggggaag aagccgccat tatcggtgat taaatgaaag
     4801 ccagtggtgt tgccatccag cggctgacca ctaccgagga acactgtacc cacgaccaaa
     4861 aaggtcacaa tggcgagcac tttaataagc gcaaaccaga actccatctc cgcaaaccat
     4921 ttcacaccga tcatattcat ggtgccaacg atggtaagtg cagcgagcgc aaagacccac
     4981 tgcggcacgc cgccaaacgc accccagtaa tgcatataca gagcgacggc ggtaatatca
     5041 acaatccccg tcatcgccca gttgatgaag tacatccagc cagcaacata agcggctttc
     5101 tcacccaaaa actcacgggc ataagagaca aaactgccac tggaagggcg gtgtagcacc
     5161 agctcaccca atgcacgcag aataaaaaac gaaaacaagc cacaaattaa ataaaccagt
     5221 gccagtgctg gacccgccat ttgcagtcgg gctcctgcac ctaaaaacaa gccggtgcca
     5281 atcgcgccgc caatggcgat catctgtacc tggcgattgc ccatcgcttt gtgatacccc
     5341 tcttcgtggg cattaagcca gcggcgtttc gcggcgtgtt gatctgaagt gtcggtgtcg
     5401 tgtttactca ttgctctccc tgattgcttt aatgaaaaag tcatataagt tgccatgaac
     5461 aatgtttatt catcctggcg atgatagtta tcaccattat tctttatggg tataaaacta
     5521 tcggcgcagc atcctacccg ttatcgatat acgatgcaaa acatcccctt acaatcctga
     5581 aggggattca tacaactgac gaaaaaatga caaatacttt tgctggttaa cctgtgtact
     5641 gtcctacact taatctttaa aagattgtga ggggcatatg attaaggtgt atggcgtacc
     5701 cggatggggc tctacaatca gtgagctgat gctaacactg gctgatattc cttatcagtt
     5761 cgttgatgtc agtggttttg accacgaggg aacttcacgc gagttattga aaaccctgaa
     5821 cccgttgtgt cagattccga cactggcgct ggaaaatgac gagattatga cggagacagc
     5881 ggcgattgca ttgatggttc tcgatcgtcg cccggacctt gcgccaccgg ttgggcgcgc
     5941 cgaacgtcag cagtttcaac ggctattggt ctggctggtt gctaatgtct atccaacatt
     6001 cactttcgcc gattaccctg agcgttgggc tcctgacgca cctgaacagt taaagaagaa
     6061 tgttattgaa tatcgtaaat cgctttatat ctggctgaat tcacaattga ccgctgaacc
     6121 ttatgcgttt ggtgagcaac taacgctggt ggactgttat ctttgcacta tgcgcacatg
     6181 ggggtctggg catgaatggt ttcaggataa tgccacgaat atcagtgcga ttgctgatgc
     6241 tgtatgtcag ttaccgaaat tacaagaagt attaaaaagg aatgaaatta tttgagtggg
     6301 ataccagagg attatgttat ttattat
//
LOCUS       DAJVRF010000184         6311 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJVRF010000184 DAJVRF010000000
VERSION     DAJVRF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.184"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="OQN13_003640"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="OQN13_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCS1616895.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="OQN13_003641"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="OQN13_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCS1616896.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="OQN13_003642"
     CDS             2408..3157
                     /locus_tag="OQN13_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCS1616897.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3729
                     /gene="yieF"
                     /locus_tag="OQN13_003643"
                     /gene_synonym="chrR"
                     /pseudo
     CDS             3179..3729
                     /gene="yieF"
                     /locus_tag="OQN13_003643"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
     gene            complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="OQN13_003644"
     CDS             complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="OQN13_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCS1616898.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5286..5951
                     /gene="yieH"
                     /locus_tag="OQN13_003645"
     CDS             5286..5951
                     /gene="yieH"
                     /locus_tag="OQN13_003645"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCS1616899.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6018..>6311
                     /locus_tag="OQN13_003646"
     CDS             6018..>6311
                     /locus_tag="OQN13_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616900.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYC"
BASE COUNT         1554 a         1605 c         1597 g         1555 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggccgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagcatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatggaac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactctgtac cgggtgggct gaaaaatgcc atcgactggc tttcccgctg
     3481 gccggtaaac cggtgttgat tcagaccagc tcaatgggcg tgattggcgg cgcgcgctgt
     3541 cagtatcacc tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa caagccggaa
     3601 tttatgggcg gcgtgattca gaacaaagtt gatcggcaaa ccggagaagt gattgatcag
     3661 ggtacgctgg accacctgac cgggcaattg accgcatttg gtgagtttat tcagcgagtt
     3721 aagatctaaa taaaaaaccc gccagtaatc atgcatggcg ggtttttaat gcgctatcga
     3781 ttttaatgag cgtcgataaa tacaatcttc aggataaaca gcagcgcaac gatgattacg
     3841 cacgggctaa ggtcacgcag acgcccggta ccaatcttca tcacgcagta ggagataaag
     3901 cccagcgcaa taccttcggt aatcgaaaag ctgaacggca tcatcacggc ggtaataaac
     3961 gccggaacag attcagtaag atcctgccag ttcacgcgag caagacttga ggtcatcaac
     4021 acgccaacgt agatcaacgc gccagctgca gcgtagcctg gcaccattcc cgccagcggc
     4081 gacagaaaga taaccagcag gaacaacaga ccaacaacca ctgccgtcag accggtacga
     4141 ccgccaaccg atacgccgga agaggactca atataagccg taacggaaga agtaccgata
     4201 aacgaaccgg tcacggaaga gatactgtcg acatacagcg cctgcttcat gcgcgggaat
     4261 ttccccttct catccgccag acctgcttta tcggtcacgc caatcagcgt accggaggag
     4321 tcaaacaagt tgaccaacat gaaagagaaa atcacccctg ccagcccgag gttaaacgac
     4381 ccggctaaat ctacatgacc cacaactgtc attacgctcg gcggcgcaga aacgatgcca
     4441 ttgtagtgca catcacccag catccagccc agcagcgtcg tcaccacgat agaaaccagc
     4501 accgctgcgt gaatgttgcg cgaggccaga atggcgatga tgaagaaacc gaggatcccc
     4561 agcagcacgc tgtgagaagt cagattaccg atgctcacca gcgtttccgg gttagcgaca
     4621 atcacacctg cgtttttcag ccccatcatg ccaatgaaca gaccgatacc gctggtaata
     4681 cccacacgca gacttaccgg aatgttagca atcatccagt aacgaacgcg gaaaatggtc
     4741 aaaagaagca gtccgacagc gccccagaag attgcgccca taccgatctg ccacggcagc
     4801 cccattgcct ggacaaccac aaaagcgaaa aaggcgttaa gccccatcgc aggagccagc
     4861 gccaccggaa ggtttgcaaa caatcccatc ataatgctac cgaaagcagc aatcagacag
     4921 gttgttacga agacggcact ggtgtccatg ccagcaacgc ccagaatttg tgggttaaca
     4981 aatacgatgt agaccatcgt caggaaggtg gtgaacccgg caatcacttc agttcgtgcc
     5041 gtagtaccat gttcgcgcag tttaaaaact cgttccagca tcccctgacc agaagtctgg
     5101 gtggtatgtt gatgactcat tatctatttc cgaacaagga gggaaaatcc gtcgctatcg
     5161 tataccaaaa tgcgacaata ggcgcgtttg tgagagactt tttttattgg atttacttat
     5221 acggcaacga ttgcgttgcg caaatcggct ctacgaaaac gttaaactga ttaaaaagga
     5281 aaggcatgtc ccggatagaa gcggtatttt tcgactgcga cggtacgctg gtcgacagtg
     5341 aagtcatttg ctcccgcgca tatgtaacga tgtttcagga atttggtatt acgctcgatc
     5401 ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta cgaaattatc gatattgttt
     5461 cccttgaaca tggtgttacg ttagcgaaaa cagaatctga acacgtttac cgtgcagaag
     5521 tcgctcggct gttcgattca gaactggaag ccatcgaagg ggctgaagcg ctcctgtcag
     5581 cgatcactgc gccaatgtgt gtggtatcta acggcccaaa taacaaaatg cagcattcta
     5641 tgggcaagct gaatatgttg cactacttcc cggataaact gttcagcggc tacgatattc
     5701 agcgctggaa gccagacccg gcgttaatgt tccatgcggc aaaagcgatg aatgtaaatg
     5761 tagaaaactg cattctggtt gatgactcag ttgccggtgc gcaatctggt atcgacgcag
     5821 gtatggaagt gttctacttc tgcgccgacc cgcacaataa gccgatcgtt cacccgaaag
     5881 tcaccacctt tacccatctt tcgcagttac cggaactgtg gaaagcgcgt ggttgggata
     5941 ttacggcata gttcttcaca ctcccttcac ttaccccgct taaattggcg ctcaaaggta
     6001 agtaaaggga gtttgatatg tctgtttcac gtcgggtaat acatcacgga ctttattttg
     6061 cggttttagg accgttaatt ggtgttctgt ttcttgtcct ctacatattc ttcgcaaaag
     6121 aaccgctggt tcttttggta ataatacagg tacttccatt gtttctctta ttgtcgatta
     6181 ctacgggagc tattcctgca ttgttaaccg gcgtgatggt cgcctgtctg ccggaaaaga
     6241 tcgggtcaca gaaaaattat cgttgtctgg ctggtggcat aggtggcgtc gttatcaccg
     6301 agatctattg t
//
LOCUS       DAJVRF010000185         6305 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJVRF010000185 DAJVRF010000000
VERSION     DAJVRF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.185"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(336..4193)
                     /gene="sigA"
                     /locus_tag="OQN13_003647"
     CDS             complement(336..4193)
                     /gene="sigA"
                     /locus_tag="OQN13_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCS1616901.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4554..5558)
                     /locus_tag="OQN13_003648"
                     /pseudo
     CDS             complement(4554..5558)
                     /locus_tag="OQN13_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5543..5920
                     /locus_tag="OQN13_003649"
                     /pseudo
     CDS             5543..5920
                     /locus_tag="OQN13_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5959..>6305)
                     /locus_tag="OQN13_003650"
     CDS             complement(5959..>6305)
                     /locus_tag="OQN13_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1616902.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         1572 a         1408 c         1411 g         1914 t
ORIGIN      
        1 gggagacaat gctgtattct tttttaaagc actcaccaga aacggtgatt gtaatgataa
       61 tggtcttctc gatcgagatg taaatacccg tttcgttata ctgtccgtat cggaaccatg
      121 agaactcagt tgcattccag ccatgctcca gaatatgtgc atttttttgc ctgatagttt
      181 ctttttttat tcgctcatgt ttaaggcata acaggcagat aaataatgtc agaaaaagaa
      241 agctaataat gagagcaaga gcgatatagt ctgtcacagg gtaatacata ttctctacgg
      301 tagaagaagg gccgcaaacg cggcccgggc tgttatcaga aagagtaacg gaagttggcg
      361 ttgatcgcgt tatccacgtt gtatttacca aatgccgatt tctcaaactc aagaccgaag
      421 cgaagattat cgcgaatttc ggcgttgaga ccaacattca tgagaatacg accgtctttt
      481 tcacctttga tacgtttctc accggacgca tcacgcagta cggtttcacc gttggcaaac
      541 aggtcaaact ggtagccaag gccggcgcgg gctgtgactt tccagtcctt accggagaag
      601 gatttaccca catcaacacc ggtacgccca atcagcggat taaagtcctt atccttcatg
      661 gtcacgctca ttccgcgatc ctgccagtcg aaccgtttac ctgatacggc cccataaacc
      721 agttctgcct gcggctcaat ccaggagtct tctgttacat gatagcggta gcctgcttca
      781 gcacccacat acaaggaatg agaactgtag tcttttgttc cgagtccagc aaaggtcgcc
      841 gagtactcat tatcatggtg aacatactta ctaatcaggt cgatataggc accagagtca
      901 aatatagcgg aagcatacag acctgccccg acggacttcg tcttgccact gaatgcatta
      961 ctgctggcat gactgtcggt atacgtcata gtcagaccag tgaaaaggtc aagtccatcc
     1021 agctcatgtt ttttatccac accaatctgc acatgggtgt agttgtcact gtatccacta
     1081 gaagctgaac ctgctccgct catgatgcgg gcccatgcac cggcctcgcc attggtgtca
     1141 cgcagatccc ccattcgttt attcaggttg ttgacttctg tcaggaaggc tttgtaatta
     1201 ccggacatat acgattttgc tgcaccggaa gatgctgtat tttcagccac cttatatccg
     1261 gtcagggtcc acgtggtatg ttcctgatcc tctttcgtgc tgatcacagg agatatgttg
     1321 ctgaaaccta tggcctgagt actggcttta aatatctctt tattgcttcc ttttggggct
     1381 gttatcaggt caacgtttaa tcctgagtta ttatttccca ctttctgtat atcgaccgac
     1441 agactgttat tgcttccggt taactgattt ttaacaatga gtttatccga ttgatttaca
     1501 gactggctca gatcagcccg cataatcaca gcagtattag tggcatccag tgtgtttact
     1561 gtcacagtat ggaaacccga attctctatg ttactaaaca acaaaagact gtcgcgggtt
     1621 tttaatgatt gcagggctga ctgtccgttt actctccata aggtgttata catgctggct
     1681 gaggcagcag gagcatcaat acctccggta taagaagtat caaagccacc cagcaaacct
     1741 gtagccagcg gtgtgtgtgt ggttttgtcc aggttatctt ctttatactg gtcaacacca
     1801 aacagaatag atgctttttt gtctgatttt atattaccat tgacccaggc ataattttga
     1861 gcctttaacg tggcaccatc ttctgtcata ttatatccat ctgacatata gattgccggg
     1921 ctgtatttat tattttctgt aggggtgcct gttaatgtca gggttccggc cagttcaata
     1981 gctttatcta catacatttt tccctgggcg gtaacagtag ctcctttgtt gataatcagg
     2041 tctgtattaa ttaaggataa tgcaccagtc atagtaacag ttgatttatc accaacagtt
     2101 attttgctgt tttgagcact cagactgccg ttgaaactac tttcgcctgc tgaatccccg
     2161 gagcgaagct gttgtctgaa tccaaaacct tcgccagtaa tgtttcttcc tgagaacatg
     2221 tcaatgtatg cagttttatt tcccagaaca atttgagagt ttttagcctc tatgtgtcct
     2281 tttacattag catcccggcc tatactcagg gttgcgtttt ctaaatttaa tttccggaaa
     2341 ttaaatttac gactttccca gtcgggctgg gaaaaagagg ctatctgatt gttggactta
     2401 tattccgtat tgtaacgttt atttacagtg ctttcctggt cctttatggc agcagaatag
     2461 tctttttgac ataaaaaagg aattggacag ttgttattgc cttctttaaa tatggcatgt
     2521 tctgtcgcat gtccttgtaa tgttaactgt gcattccgga cagagatatc gtttttgata
     2581 tcgactgagc catcaaagat cagattggca ttattgttat gttgctgggt agtattgata
     2641 atatgattta tgcttatatt acctgataca ttcccatggt acatatatgc atcagtatta
     2701 gtaagtgata atgttgattg cttcacgttt gagttcgtta cggttgttcc cgcatctgtt
     2761 gctgcgattt tctgaaatga ctggtcatag ccgtttagat ccagtgttcc gccatttctg
     2821 gtaaaataga tttcgccatt accgctttcg cttaaagcgt ctttggcatt tatttttacc
     2881 gtacctttac ctccggccat gtaaatttta ttgaaggctt tttcagcact aaggatgaca
     2941 gtcccattac ctattttaag gttatttccc tgtgctattt ttacatccag agtaccacta
     3001 cctattttat gcagattatc tccggcaacg cccttaacat tccattcaac ggtggtatta
     3061 ttatcaatat ctatcccggc acctttataa gacttatctt ttccgtacac tttatatgtc
     3121 tggttcttat cgaatataag cccgccaatg ccaagatcgg tatcctgctt gagcgttaat
     3181 tcccctcctc ctgagacaga caggtcctta tcttttataa tcgatgataa ctcttgtttt
     3241 ttaccatcca gctgcatagt gtttgtgaaa ttctttttta aattgtttac cagaatatta
     3301 ttaaataacg agtagtttgt ccaggctata ttggtactac tgagaaaacc aactgtactg
     3361 gtaacaccat ggaaaaccca tttttgttgt tctttatcat atagataaga accagaacca
     3421 ctatcgccaa aaattggtga gtttttaaaa ggggttctgt tggtataaat gtaactcttg
     3481 tttttccagt ccagattaaa aagacttgcg cttaacaagt cgggattata gttttgtcca
     3541 aacagatatt gtttgccgtt taatatcgtt gaggttgttc cggcacctgc tctgaagcca
     3601 attatttgtt ccttaccgtt gtagtttacg ccatatcttt tcatcgcatc ttcgggagaa
     3661 agtgagaagt cggcactctc cgtaacccct gttgtttcca cgacgaattt attcagacga
     3721 tgaaccgaaa agtcgccaga tgatactctg ttacttgctt tatatgctgt ctgatcccat
     3781 gactgtgttg ttattgcatg atgctgtgta ccgttatgag tcgcagtaac actgtacgca
     3841 cctccaattg atgttgttgc acctttgttg gaaacagcag aaaaatcagg tacaggtagt
     3901 tctggaaaat gaaatatctt tccatctttc atcattaatt gaacatttgt tgcacccggt
     3961 ctgaactctc ctctgttttg agcaaggtca agataatcgc gggtccatat attatttata
     4021 tccagcattg ctgcaaatgc tgtttcgcca taacttccat atattgagga taaaataata
     4081 ccgaggataa ctttctttct tgatgtaccg acactaactt tccgggtcag ttcagaaaca
     4141 gcaactaatc cacctgtaat atgactatat ttcagtgaat aaattttatt catacgtgaa
     4201 ctccgatatt tctgagttgt ggttatggga taacatttaa ttacaacggg gaactatcag
     4261 tcatggtacg taactgataa gggtataatg ataatcactg tcagttatat ttgtatgaca
     4321 gatgtaacaa aatgaaaaaa aagataagca caagtgattg attggtcaga tattgatcac
     4381 ttggtgtttg tattttgaac tttgcgatca aaatttgatc tttgttgttt ccggtcaatc
     4441 ctttacaggt tttctgtatt atccggggga gttaagtctg tcgatgtccg acagactttt
     4501 gatgatggaa acaacatgca gcgataatgt gcatgctgtt ttgcaggtgg aactcagaag
     4561 gtgaactgga actgtgcgac cgcaccgtaa gtgttgtccg tgccgatcag gcctgcaaca
     4621 tcaaggtgaa ccacatcaaa cggtgagatc cccaccccgg ccgtgaatgc actgccgtcg
     4681 ttaccggcca gattctgacg gtaaccggcg cgcagctgtg cccatttcca ggcattaaat
     4741 tctgcgccaa tggcggcaaa ctgacggttg ctgtcggagg tgaaaccgct ggccggggtc
     4801 agatccacat caaatgcggt ggtgaacatc gcgttgtgcc aggagacacc ggccgtcacc
     4861 tgcgggcgga tcctgaaggt ttccgtgata ccgttcactt cttttgtctc aatactacgg
     4921 gggatcaggt tctgtgcgac cagtcccagc gtccagttgt catccaggtc cgtactggca
     4981 ccgatgtcgg cgttgatgcc atttttcgtg ttgtggtagc ggtcgccttt gaaggcgctg
     5041 ctgtcataat ttctgaccag cacgttgtag ttgaacaggt cgacgcgctg gaatttgggg
     5101 gggaagccca gagaccattt ctgcccggcc gtttccagtt ccgtagccag agcaataccc
     5161 acatcagtga tcagtgccgc gcgggcaaag gcctgtgatg tcaggcggct tttgtccacc
     5221 tcctgcccgg tgctgtttgc cacctcttcc aggtaagtga ggtcagcatc agagacttta
     5281 ccgtcgacac tgacggtgcc gtgtgattta accatcagtg cggcagagag gcgatcaccg
     5341 ggaagggccg ccacagcaga aactcccagc tgtgcggcgg catgtgtgtt acggaagtcc
     5401 cggaggtgtt ctttcaggcg tgcggcagcc tcaggaacac cgtggttact ttcgatggcg
     5461 cggtcgaagg ccttccactc atcactgatc cggtcggcgt tgtcggtgat attatccgga
     5521 ttggagagct gcgcgccgac ggacggaagc aggatgctgg gctcatgcgc gacgcaaaat
     5581 ccacgatgta tatatcagta ctaaaagcgc gacggcggaa gaagctctga aactaatcgg
     5641 tgaactgtac gccatagagc acgatatacg cgggttaccg gtgtctgaac gcctggcggt
     5701 caggcaaatg cagagtagac cgctcctgac ttccctgtat aagctgatgc aggagaaaga
     5761 acacacgtta tcgaagaaat gtcgtctggg agatgcgttc cggtatatca ggaagcactg
     5821 ggctgcgttg tgcaacttct gtgatgatgg cctggctgag gtagataata atgcagcaga
     5881 aagagcgctt cgtgcagtct gtctcgggaa gaaaactttt tcgaggatca ctcattccgt
     5941 gtctgactgc ttcagggttt aattttgctt ctgctgactg aatgatgatt gggcagcaag
     6001 tgcaatatcc cacataaatc cagttaactc acgagccacc gccgtaatgg ctacttgtga
     6061 ttttttccct tttgctgtca gagttcggta acgagaacac aatcgttgtt gagctttcca
     6121 tgcaatatct ttggaatatt gtgttacaga gtccggtcgg taatatataa gccatgctcc
     6181 aacttttgca ggtgtacgat aagaccaagc ggcttcgtaa agcagacgtc tcagttcgct
     6241 gtttccaact tttgttattc ctctgggacg aatactgttt ccactggaat attcacctgg
     6301 cacca
//
LOCUS       DAJVRF010000186         6235 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJVRF010000186 DAJVRF010000000
VERSION     DAJVRF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.186"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            101..931
                     /locus_tag="OQN13_003651"
     CDS             101..931
                     /locus_tag="OQN13_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HCS1616903.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1072..1845)
                     /gene="uxuR"
                     /locus_tag="OQN13_003652"
     CDS             complement(1072..1845)
                     /gene="uxuR"
                     /locus_tag="OQN13_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCS1616904.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2060..3520)
                     /locus_tag="OQN13_003653"
     CDS             complement(2060..3520)
                     /locus_tag="OQN13_003653"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCS1616905.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3601..4785)
                     /gene="uxuA"
                     /locus_tag="OQN13_003654"
     CDS             complement(3601..4785)
                     /gene="uxuA"
                     /locus_tag="OQN13_003654"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCS1616906.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLVQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5125..>6235
                     /gene="gntP"
                     /locus_tag="OQN13_003655"
     CDS             5125..>6235
                     /gene="gntP"
                     /locus_tag="OQN13_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCS1616907.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISA"
BASE COUNT         1474 a         1616 c         1583 g         1562 t
ORIGIN      
        1 aacagaaaca tgcatgtcat ttgtttactt ctctttttac tacagaaaaa tttatctatt
       61 tttctaaaca tcagtgaaat actcataaaa ggtaactatt atgtctatgc cattaagcaa
      121 tgcccttcag agtcaaatta ttacagataa tcacttcctt catcacccaa tagtcgacag
      181 tgagtttaca cataaattaa aatatgcacg catggatttg gaaaacatct atcttccgcc
      241 tcttactcgt ggcaataacc ataattacga tggaaaaagc gctgtcgaaa ttagaaaact
      301 tgatattagt aaagagtcct ggccatttaa ttatgtgaca ggagcatgca gagaatccga
      361 tggtattaca acaacaggac gtatgctcta tcggaacttg aaaatcacct cagctttaga
      421 cgaaatatat ggtggcatat gtaaaaaggc gcatgctgcc acagagttag cagaaggttt
      481 acggctaaat ttatttatga agagcccttt cgatcctgtt gaggactata cagttcatga
      541 aataacatta ggccctggtt gcaatgttcc tggttatgcc ggaaccacga taggatatat
      601 ttcaaccctt ccagcttctc aggcaaaaag atggaccaat gagcagccaa ggattgatat
      661 ctatattgat cagataatga cggtaacagg ggtagcaaat tcttccggat tcgcccttgc
      721 cgcattactt aatgctaata ttgagttggg taatgaccca ataattggta tagaagccta
      781 tcccggtacc gcggagatcc atgcaaaaat gggatataaa gttatcccgg gtgatgaaaa
      841 tgcaccactc aaacgtatga ctctgcaacc gtcatctctt ccagaactgt ttgaattaaa
      901 aaatggtgag tggaattata ttggtaaata actaccagga agaatgagta ctaacattca
      961 ggttatacag atagctaaac aataccctgc aatttacaaa gtaatactca ttgcgacttc
     1021 ttaatttaag aaagcgcaag gaacatttac ccttgcgctt attataataa gtcaggcgtc
     1081 aactttatcc agcggccatg aatcaaacag atagccatca aaataaatat cgtcaacgtt
     1141 cgagaattcc agcagacgtt gcttaacgtt ttccagatgc cgccacattg ccagcttcgc
     1201 cgctcgggca tcttttttga ttaacgcggc gaggatctgt ttgtgatcgc ccaaccactc
     1261 tttgcgatac aggctgtcat ccagatggct gtgcaactgg atccacattg gattgttttc
     1321 ccgccactgc caggactgac ggaacagctc caccagcatg ctgttatgcg ttgcttcggc
     1381 aatggcgaga tggaactgca tatcaccact ttcgctgctg cccggcgcgc tggaagccag
     1441 ctcacgctct tccagttgca atgcctgacg cattttgacg atatcttcgc gggtagcctg
     1501 caaagcggca aattccgcga tgttactttc cagtaactga cgcgcctgta acagctcaaa
     1561 aggaccggca tcattgcaga cgttagcatc cggactgtct gtgttgtggc tgcctgagct
     1621 atcaagaaca tagataccgg caccccggcg cacttccacc agccctttga tctccagcat
     1681 gatcagcgct tcacgtacca ccgtcctcgt gacatcaagc atttctgcaa tttcacgctc
     1741 cggcggcagc cgttcgccag gattgtatgg cgtctttatg atcagatcgc ggatcatcgc
     1801 cccgacttcc tggtaaggtc tttgcgcaga ggtggcagat ttcatggtgt tgtgtccgtt
     1861 aggtgcggtt aatcaaattt ttgccggatg cggcgtgaac gccttatccg gcctacaacg
     1921 ggcaatttta cggcacccgt taatctcaaa ctataccatt agcactggaa gttggcacgt
     1981 acaggtgggc aaaaaaacga aaactggtca accaaatatc agtttgattg accagtaagt
     2041 ctgtagggta atcagttagt tacagcgcag ccacacactc gcgcgcaccg cgttcgcaca
     2101 gctgctgata tgccgccgtc accgcgccaa caaagtcggc attctgcggc agatcatcgg
     2161 caaaaatgcc gctcaggccc agcagcgctt tcacgcggtc tgcgccctga tactgcgcgt
     2221 taatcttctg gaactcggcc agcatcgggt caaccacgtc aatggcatta ccctgttcat
     2281 ccacgccctg ggtgtagcgc atccagccag ccacgcccag tgccaggtga cgccagctgc
     2341 cgccgttttg caggtgcaga cgcaccggat ccagaagacg ctgcggtaac ttctggctgc
     2401 cgtccattgc aatctgccag gtacggtgac gcagagacgg gttgctgaaa cgctcgatca
     2461 gcagcgtcgc ataggcgttc aggtctgtac cttccggcat cgacagcgtt ggcgcttgtt
     2521 cctgcatcat cagggcaaag gccgctttgc gataatccgg attagtcatg gtgtcggcaa
     2581 tggtttcata gccgccgagg taaccgaggt acgccaggaa agagtggctg ccgttcagca
     2641 tacgcagttt catcatttcg aacggcacaa cgtctgcaac gaactgtgcg cccactttat
     2701 cccagtccgg acgaccatta acgaagttat cttcaatcac ccactgacgg aacggttcgc
     2761 aggcaatggc gcacgggtcg taaacaccca gctggtcagc aatttcctgt aaggtttctg
     2821 gcgtcgccgc cggaacgatg cggtcaacca tggtgcacgg gaaggtgacg ttctcttcaa
     2881 tccatgccgc cagctgcgga tcacgtgcct gagccagccc cagaaccgcg accttcgcca
     2941 catgaccgtt ttcacgcacg ttgtcgcagg acatcaccgt aaacgctttc agcccttttt
     3001 cacgacgcag acgcaaggct tcgacgatgt aaccaatcgc ggacttcggc gcagtcgggt
     3061 tttccagatc gtgcttgatc agcgggttat tgagatccag ctgaccgctt gccgcatcgg
     3121 cgcaataacc tttctctgtg accgtcaggg agacaatcgc cgtctgcgga cgcgccatcg
     3181 cgttgagaat accttcacaa ccgtcgattt ccggatgcag ggcttctttc atcgaaccga
     3241 taattttcag ctcggtgctt tctgcgcctt tttccgccac ggtgtacagc agttgctgtt
     3301 ttttcaggtt ttcaatcagc acacggtcgt tgcctggcat caggttaact tcgcagatac
     3361 cccagtcgct gtcggtactt tccagcagat gatgggtgta cagcgcctgg tgcgcgcggt
     3421 gaaacgcccc gcaaccgaga tgcacaatgc gtgattccag acgagaatga tcccatgacg
     3481 ggcgggcaac cggcagattg ctgtcaacaa tagtagtcat cgtaaactcc agaatccgtc
     3541 ttcagagagt gacggcaagg aagaaggaac cggcacggcg cgcagccatg ccggtgggta
     3601 tcaacggcta aagaaagcgc gctggatcgc cagttcgaca ccgcgaactt cagccaggcc
     3661 tttcagacga ccaattgcgg agtaacctgg gttggttttc ttcttcaggt cgtccagcat
     3721 ctgatgaccg tggtccggac gcatcgggat caggtcttct ttgccttccg ctttacgacg
     3781 gtgttcttct tcaacaatcg ctttcaccac ttcgtacata tcaacgtcac cgttcaggtg
     3841 cgccgcttcg tggaaggttt tcgggttatc ttcacgcatg gtggagcgca gatgggtgaa
     3901 gtaaatacgc ggaccgaact gcttgatcat atcaaccaga tcgttgtcag cacgcacgcc
     3961 gtaggaacct gtgcacatgg tgaaaccgtt cgccatgctg tttacggtat caaccatcca
     4021 ctgcatatct tcaatggtgg aaacaatgcg cggcaggccg aggatcgggc gcggcggatc
     4081 gtccgggtga acagccatac ggacgccaac ttcttcggca accggaataa tcgctttcag
     4141 gaagacggca aagttttcgc gcagtttggc tttgtcgata tctttataca gctccaggtg
     4201 cttacggaac tggtcgaggg tatacccttc ttccgcgccc ggcagaccgg caatgatgtt
     4261 acgggtcaga cgcgctttgt cttcgtcgct catagtggcg aagcgttcag cggcctgagc
     4321 aatttcttct tcggtgtaat ccgcttccgc gcctggacgc ttcaggatat gcatttcgaa
     4381 tgcagcgaat tcgatctggt cgaagcgcag agctttggag ccgtctggca gcacgtattc
     4441 gaggtcagta cgggtccagt cgagcaccgg catgaagttg tagcacacgg tgcgaatgcc
     4501 gcactgcacc aggttgcgca gagtctgctg atagttagcg atccactgtt catagttgcc
     4561 agtgtgggtt ttgatatctt cgtgaattgg cacgctttct acgacagacc ataccaggcc
     4621 tgcgtcttca acgatcgcct tgcgtttgag gatctcttct acggaccata cttcgccgtt
     4681 cgggatatgg tgcagcgcgg taaccacgcc agttgcgccc gcctgacgga catcagctag
     4741 agaaaccgga tcgtttgggc cgtaccagcg ccaggtctgt tccatgattt ttcctctttc
     4801 acatagtggg ttccacagca aagagacgtg gagccccgga gttgtttagg tatccggatg
     4861 aacaaccaga ccgcagccgt ggcgttgcat cggatgagat ggcgtataag ttctaccttt
     4921 tggtaagaca tgtcaacccg aaatctgaaa ttggttaacc acatcgcaag aatttcactc
     4981 tcacggttgt tgctgcaaat tgcagcaaag ggtaatcgat ggttattgat cagaggatgt
     5041 gacattcatc gcaacaatgg ttgaccaatt tacataacat gtcgcccaaa ataacaacgg
     5101 ttcaaccagg taaggtaaaa cgttatgcat gtgcttaaca ttctctgggt ggtattcggc
     5161 attggcctga tgctggtact gaatttgaag ttcaaaatca attcaatggt ggctttgcta
     5221 gtggcggcgc tgtccgtcgg gatgctggcg ggcatggatt tgatgtcgct gctgcacaca
     5281 atgaaagcgg gcttcggcaa cacgctgggg gaactggcta tcatcgtggt gttcggtgcg
     5341 gtcatcggta aattgatggt cgactccggc gcggcgcacc agatagcgca tacgctgctg
     5401 gcgcgtctcg gtctgcgcta tgtacagctg tcggtaatta tcatcggcct gattttcggt
     5461 ctggcgatgt tctatgaagt ggcctttatc atgttagcgc cgctggttat tgtcattgcc
     5521 gccgaagcta aaatcccgtt tctgaaactg gcgatcccgg cagtagcagc tgccactacc
     5581 gcacattcac tgttcccacc gcagccgggt ccggtggcgc tggtgaatgc ttatggcgcg
     5641 gatatgggga tggtttatat ctatggcgta ctggtgacga tcccaagtgt catctgcgca
     5701 ggtctgatcc tgccgaagtt cctcggcaat cttgagcgcc caacgccatc attcctgaaa
     5761 gcagatcaac cggtagatat gaataatctg ccctctttcg gcgtttcgat tctggtgccg
     5821 ctgatcccgg cgatcattat gatctctacc accatcgcca atatctggct ggtaaaagat
     5881 acccctgcct gggaagtggt taactttatc ggttcctcgc cgattgcaat gtttattgcg
     5941 atggtggttg cattcgtact cttcggcacc gcgcgcgggc acgatatgca gtgggtgatg
     6001 aacgcttttg aaagcgcggt gaagagcatt gcaatggtga ttctgatcat cggtgcgggt
     6061 ggcgtgctga agcagaccat catcgacacc ggcattggtg acaccatcgg catgttaatg
     6121 tcccacggca atatctcgcc ctacatcatg gcatggctga tcaccgtact gattcgtctg
     6181 gcgacgggtc agggtgtggt ttcagcgatg accgccgccg ggattatcag tgctg
//
LOCUS       DAJVRF010000187         6063 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJVRF010000187 DAJVRF010000000
VERSION     DAJVRF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.187"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..436)
                     /locus_tag="OQN13_003656"
     CDS             complement(<1..436)
                     /locus_tag="OQN13_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1616908.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFADEAVSGSVK
                     ASLRNGFSSLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGR"
     gene            1023..1370
                     /locus_tag="OQN13_003657"
     CDS             1023..1370
                     /locus_tag="OQN13_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCS1616909.1"
                     /translation="MILDSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFI
                     GSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEI
                     GAVPAIPQQSSLF"
     gene            1423..1818
                     /locus_tag="OQN13_003658"
     CDS             1423..1818
                     /locus_tag="OQN13_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCS1616910.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1859..2602
                     /gene="cmoA"
                     /locus_tag="OQN13_003659"
     CDS             1859..2602
                     /gene="cmoA"
                     /locus_tag="OQN13_003659"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCS1616911.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            2599..3570
                     /gene="cmoB"
                     /locus_tag="OQN13_003660"
     CDS             2599..3570
                     /gene="cmoB"
                     /locus_tag="OQN13_003660"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCS1616912.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(3735..>6063)
                     /gene="torZ"
                     /locus_tag="OQN13_003661"
     CDS             complement(3735..>6063)
                     /gene="torZ"
                     /locus_tag="OQN13_003661"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCS1616913.1"
                     /translation="DGKILTAERWGAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQ
                     VHTTTRIQHPMVRKSYLDNPLQPTKGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPS
                     AIFAGSYGWRSSGVLHKAQTLLQRYMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVY
                     EQQTSWPLILENSQVVVLWGMNPLNTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPI
                     RSETIDFFGDNATWIAPNMGTDVALMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYL
                     RGKSDNTPKSAAWAAEITGVPEAQIVKLAELMAANRTMLMAGWGIQRQQYGEQKHWML
                     VTLAAMLGQIGTPGGGFGFSYHYSNGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRA
                     IPVARIVDALENPGGKYQHNGKEQTYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEM
                     IVVSECYWTAAAKHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDF
                     DVFADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKL
                     IEMRRSEKNEQYVRYGDFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAP
                     DEWKGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDAARFSIAN
                     GDLVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVLTADIPSS
                     QLANACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
BASE COUNT         1416 a         1557 c         1419 g         1671 t
ORIGIN      
        1 gtcttcccat atgtacccct tcagctttag cacgttcaat accagccttt ctacgctctg
       61 ctatcaagct tctttcaagc tgtgccaacg ttgacataat gcctaacatt gcttcaccca
      121 tagcgcttga taagtcaaat ccttctctaa ggctgataac tgtcacacct ttagcctgta
      181 gcgctttaac cgttgataac acatcaattg tgtcacgtcc aagacggtct acagctacca
      241 caactacggt gtcaccttct cgtgcataag ctaacaaact gctgaaaccg tttcttaaag
      301 aagctttgac agaaccactg acagcttcat cagcgaacca tttagcaatg ttgtaccctg
      361 cctcttcaat ttgcatttgc tggttagtga tggattggtc atctgtactc accctgcaat
      421 atccaagtat cgccataatg ttccccttag aagtctcaaa agttagtctc ataagcttag
      481 ctgtctcata atccaaaaac aaccttttga gactacttga gggtgcattc taccgttacg
      541 ttgaaagtgt ctcaaaagga cttccttatg agactggtta atagagcgct atgaagattc
      601 aggagggtaa ctataaccat cactttctaa ctttcacgta ggaaatcgtt aagtttcttg
      661 atagttacac catgtgacca actatctata tatcgttata tagccctgta tatagcacta
      721 tatgtagtgt ctggagaggg acgactaaca cgatacagtt taccctgaaa agagacttca
      781 cagattcttt atatatttca ataagttatg ctaaaaagag actataccga tactgcatag
      841 aatttgtgta ggattgcata gatacttgga aaaacaccgt taaattgcat agtcattcac
      901 ctgatattgt cctgttttta caggtagttg attcggatgt tctaggaatc atcctcatag
      961 ttgattcggt tagttgggct tgtaccaacc cctaatcaac ctcatagtgt atttagacct
     1021 tgatgatttt agacagccgc cctgttcatg cagcacgtcc acacagtgaa gctattcgcg
     1081 acgctcaacg aaaaaaacct aaagttccgg tacatgctgt actgacggcg acaaatccgc
     1141 tgattcgttt tatcggtagt gatgatatga cgcaaaaccg ggaattattt caggtctggt
     1201 tacagaaatt agcgcagtgg catcagacca ctacgcctta tcttttttta catacgccag
     1261 acattgccca ggccccggaa ctggtacata ccctgtggga agacttacgt aaaacgcttc
     1321 cagagatcgg agcagttccg gctattccac agcaatcttc tcttttctga atttgccacc
     1381 tatcatagac aggtgccatc ggccatttta aagggagttt gtatggtaag cgcgctatat
     1441 gccgttttaa gtgcgttgtt attaatgaag ttctcttttg atgtcgttcg cctgcgaatg
     1501 cagtaccgcg ttgcctatgg tgacggtggt tttagcgaac tgcaaagcgc tattcgcatt
     1561 catggtaacg cggtggaata tattcccatc gcgattgtgc tgatgctgtt tatggaaatg
     1621 aatggcgcag aaacctggat ggtgcatatt tgcggcatcg ttttgcttgc tggtcgtctg
     1681 atgcattatt acggttttca tcaccgtctg tttcgctggc gacgttccgg catgagcgcc
     1741 acctggtgtg cgctgttgct gatggtgctg gcgaatcttt ggtatatgcc ctgggagttg
     1801 gttttctccc tgcgttagcg cacaatacgc cactttcttt ttcccggatt tttacgttat
     1861 gtctcaccgc gacacgctat tttctgcccc tatcgccaga ctgggcgact ggacctttga
     1921 tgaacgggta gctgaagtct tcccggatat gatccagcgt tccgttcccg gctattccaa
     1981 tattatttcc atgattggta tgttagccga gcgcttcgtt caacctggta cgcaggttta
     2041 cgatctgggt tgttctctgg gcgcggcgac gctctcggtg cgtcgcaaca ttcatcatga
     2101 taattgcaaa attattgcca tcgacaactc cccggcgatg attgaacgct gcagtcgtca
     2161 tattgacgcc tataaagccc ctacgccagt agacgtcatt gaaggtgata ttcgcgatat
     2221 cgccattgaa aacgcatcga tggtggtgct gaattttacc ctgcaattcc tggaaccttc
     2281 cgagcgccag gcattactgg ataaaattta tcaaggactg aacccgggcg gcgcgctggt
     2341 gctttcggaa aaattcagtt tcgaagatgc caaagttggt gaactgttgt tcaacatgca
     2401 ccacgacttt aaacgtgcca acggttacag cgaactggag atcagccaga agcgcagcat
     2461 gctggaaaac gtgatgctga ccgattccgt ggaaacccat aaagcacgcc tgcataaagc
     2521 cggttttgag catagcgagc tgtggttcca gtgctttaac tttggttcac tggtggcatt
     2581 aaaagcagag gacgctgcat gatcgacttt ggtaactttt attctctgat tgccaaaaat
     2641 catctttcac actggctcga aacgctgccc gcgcagattg ctaactggca gcgcgagcag
     2701 cagcacgggc tgtttaagca gtggtccaac gcggtggaat ttctgcctga aattaaaccg
     2761 tatcgtctgg atttattgca tagcgtaacc gccgaaagcg aagagccact gagcaccggg
     2821 caaattaaac gcattgaaac gcttatgcgg aacctgatgc catggcgcaa agggccgttc
     2881 tcactgtatg gcgtcaacat cgataccgaa tggcgttccg actggaaatg ggatcgtgtt
     2941 ctgccccatc tttctgattt aaccggacgc accattctgg atgtcggctg cggcagcggt
     3001 tatcacatgt ggcgcatgat tggcgcaggc gcgcacctcg cggtggggat cgaccctacg
     3061 caactgtttc tgtgccagtt tgaagcggtg cgtaaactac tgggtaacga tcagcgcgca
     3121 catttgttac cgttaggtat tgaacagctt ccggcactga aagcctttga taccgtcttt
     3181 tcgatgggcg tgctttacca tcgccgttcg ccgctggagc atctctggca gttgaaagat
     3241 caactggtga atgagggtga actggtgctg gaaacgctgg ttattgatgg cgatgagaac
     3301 acggtgctgg taccaggcga tcgttacgcg caaatgcgta atgtctattt catcccttcc
     3361 gcgctggcgc tgaaaaactg gctgaagaag tgtggttttg tagatatccg cattgtggat
     3421 gtgtgcgtta ccactactga agagcagcga cgcactgaat ggatggttac cgaatctctg
     3481 tccgatttcc tcgacccaca tgatccgagt aagacagttg aaggttatcc tgcgcctaaa
     3541 cgcgcggtgc tgattgcgcg caagccgtaa aggtctggta ttactgccgg atgcggcgtg
     3601 aatgccttat ccggcctaca aacccttgct aattcaatct attgcaggga ctatgtaggc
     3661 ctgataagca tagcgcatca ggcagtttta cgtttgcata acctcagcgc ccgtttccgg
     3721 gcgctattca cttcttacgc ctgaacagca ggcttatcaa atgccgttaa cttcggtgcg
     3781 ttacccgtat atttttcgat atacaccagc gcagagttac cggcacaggc atttgccagc
     3841 tgcgagctgg ggatatccgc cgttaacacg ttcgcactgc cgtttttaca caagccgttt
     3901 tccagatctg gccatgcacc ttcatgcacg cataccacgc cttttttgat cccgtcagtc
     3961 accaccgcgc ctgtcaaaat ctgaccacgt ttgttccaga cacgcaccag atcgccattc
     4021 gcaatactaa atcgagcagc atcttcggtg tgaatagtga tcggttcacg atcggcaacc
     4081 gcatattttt tacgcagttc cgcatagtta agctgactgt gtaaacggtg tgccggatgt
     4141 gcggtcagaa gctgcaactg cttctcgtcg gcggtaccct tccactcatc aggcgcaagc
     4201 caggttgggt gtgccgggca atccttatag ccaaattttt ccagcgtttt ggagtaaatc
     4261 tcaattttgc cacttggcgt acccagcgca tttttcaccg gatcggcgcg gaaatcacca
     4321 taacgaacgt actgttcgtt cttctcgctg cggcgcattt cgatcagttt attttgctgc
     4381 cagaaggcat taaacattgg catagtgacg cgttgcgcac gggccccttt ctgagcggca
     4441 tcatagaaaa atttcagcca cgccatttca tctttacctt cggtatagat ctcttttccg
     4501 ccaggtttga gtaattccgc aagatcggca aacacatcaa aatcgtttcg cgcttcaaat
     4561 tgcggagcaa cagcctgctt catcggcaca atatgctggt tgctgtaatc accggtcatc
     4621 gtcaagtcat tgcgctcaaa cgatgtggtg atcggtaata cgatatctgc gtgtttagca
     4681 gctgcggtcc agtagcattc agaaacgacg atcatctccg gtttctgcca tgctttaatc
     4741 agacgattag tatcctggtg atgggtaaag ttaccgccac ccgcccacca gatcattttg
     4801 atattcggat acgtttgctc tttaccgtta tgctgatatt tgccgccagg attttccagc
     4861 gcatcgacga tacgggcaac aggaatcgcc ctcattccgc catcatcagc ggcctcgctg
     4921 gcctgtccgg caatcgccgc agacatttcc ggcagcacgc cgccaacacg tgtcgggttg
     4981 ccaccattgg agtaatgata agagaaacca aaaccgccgc ccggtgtacc aatctgccct
     5041 aacatcgccg ccagcgtcac cagcatccag tgtttctgtt caccatattg ctggcgctga
     5101 attccccagc ctgccatcag cattgtgcga ttcgccgcca tcagttcagc gagtttgact
     5161 atctgcgctt ccggtacgcc agtaatttcc gccgcccagg ccgcgctctt cggcgtatta
     5221 tcgctcttac ctctcagata ctcttcaaac tgcggatacc cggtagtgta tttttcaagg
     5281 aacactttat cgtgtttgcc ttgtgtcatc agggtatgcg caatccctaa catcagtgcc
     5341 acgtcggtgc ccatattcgg cgcgatccag gtggcattat cgccaaagaa gtcgatggtt
     5401 tcggagcgga taggatcaat ggcaatcact ggtttgccag attttttcag ctgatggaag
     5461 tattccagcc cttgctcatc ggtactgctc caggcaattt ttaaggtatt cagcgggttc
     5521 atcccccaca gcaccacaac ctggctgttt tccagaatca gcggccagga ggtctgctgt
     5581 tcatacacct ctacagaacc gaccacatgc ggcatgatca cctgtgccgc cccggttgaa
     5641 taatcgccgc tatgcccgga ataaccgccc gccaggttca tataacgttg cagtaaggtt
     5701 tgcgctttat gcaacacgcc agaagagcgc cagccgtatg atccggcaaa aatagccgat
     5761 ggtccgttag ctttgcgaat gcgatcgtgt tgctcatgaa tcagttttaa tgcttgttcc
     5821 cagctcacct gtacataggt atcttcgcca cgacctttcg tcggttgcag tggattatcg
     5881 agatagcttt ttctcaccat cggatgctga atacgcgtcg tggtgtgtac ctgatccgcc
     5941 gccgtagact gtaaggaatt cggtatggtt ttcgccagcg cgcctgttga agaaacaatc
     6001 ttgccgtcct tcacttctac gttcatcgct ccccaacgtt ccgcggtgag gattttaccg
     6061 tcc
//
LOCUS       DAJVRF010000188         6048 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJVRF010000188 DAJVRF010000000
VERSION     DAJVRF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.188"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..286
                     /locus_tag="OQN13_003662"
     CDS             <1..286
                     /locus_tag="OQN13_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1616914.1"
                     /translation="RQLEYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFR
                     CQVCGYTVNADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            complement(326..997)
                     /locus_tag="OQN13_003663"
     CDS             complement(326..997)
                     /locus_tag="OQN13_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCS1616915.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1104..1337)
                     /gene="yedF"
                     /locus_tag="OQN13_003664"
     CDS             complement(1104..1337)
                     /gene="yedF"
                     /locus_tag="OQN13_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCS1616916.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1334..2539)
                     /gene="yedE"
                     /locus_tag="OQN13_003665"
     CDS             complement(1334..2539)
                     /gene="yedE"
                     /locus_tag="OQN13_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCS1616917.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2726..3139
                     /locus_tag="OQN13_003666"
     CDS             2726..3139
                     /locus_tag="OQN13_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1616918.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEEIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3173..4660)
                     /gene="amyA"
                     /locus_tag="OQN13_003667"
     CDS             complement(3173..4660)
                     /gene="amyA"
                     /locus_tag="OQN13_003667"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCS1616919.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4738..5103)
                     /gene="fliT"
                     /locus_tag="OQN13_003668"
     CDS             complement(4738..5103)
                     /gene="fliT"
                     /locus_tag="OQN13_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCS1616920.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5103..5513)
                     /gene="fliS"
                     /locus_tag="OQN13_003669"
     CDS             complement(5103..5513)
                     /gene="fliS"
                     /locus_tag="OQN13_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCS1616921.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5528..>6048)
                     /gene="fliD"
                     /locus_tag="OQN13_003670"
     CDS             complement(5528..>6048)
                     /gene="fliD"
                     /locus_tag="OQN13_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCS1616922.1"
                     /translation="KEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAFK
                     TLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKGY
                     LADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTST
                     YLSQQFTAMSNS"
BASE COUNT         1479 a         1593 c         1503 g         1473 t
ORIGIN      
        1 ccgccagctt gagtacaagc agctctggcg tggcggtcag gtgcttgctg ttccgccagc
       61 gtacacaagc cagcgttgcg cgtgctgtgg tcatacagcg aaagaaaacc gcctgtcaca
      121 aagtaaattc agatgccagg tatgtggata tacagtgaac gccgatgtaa atggcgctcg
      181 taacatttta gcggcggggc acgccgttct tgcctgtgga gagatggtgc agtcaggccg
      241 cccgttgaag caggaaccca ccgaaatgat tcaggcgaca gcctgaacgt agcagggatc
      301 ctcgtccttc agggcgtgga ggatgtcaac aaacaggttg aattaactcc gcgccctggt
      361 ttttgacatt acctaccgcg cgcgataccg ggtgccaggt gaactggttt gctggcacac
      421 agccgttggt agcaatttct gatgcttctt taccgccaat ctcttgccgc atccattcct
      481 gtgcagcttc tggcgacaga accagcgggc ggcggtcatg aatgtctacc agaccctggt
      541 ctgctgcagc agtgacaatt aaaaatcctt cggcttcgtc accacgctca aatggtgtgc
      601 tacctatcgc ggccataaaa ataggttgtc catcagcacg atagataaaa aaaggctgtt
      661 ttttgtcgcc ttcttttttc cactcaaacc agccatcggc aaaacagatt gcccgaccat
      721 gttgccagag cggtttaaac atgcgactgg ttgcggcagt ttctacgcgg gcgttaatca
      781 gcggcggttt atcccaccat ccgggagcat atccccagaa aaccggatcc agatgaaggt
      841 gttcatcacg ttcactgagc agaagaactt tggttcccgg cgcgacgttg tatctgccaa
      901 tgggttcggg atcgtaggga atatcgcgtt caatatcttc cgcgagaagc gcaaggtaat
      961 cttcacgcgt ttgggattgg gcaaagcgtc cacacataga aacctcctga gatcatactg
     1021 aaaagaatag gagagggggc gggtaaaatc tatgtgtcag aggcaacaat gttcccgctg
     1081 tgggccacag cgggaatgag ggattacttc tgaattaaat aacgaatggt cggcccgtct
     1141 tgctgaatat ccagcaccgt atagccgtga ttacgcgcat ccagtggaat attattgatc
     1201 gactgtggac agtcgctcac cacttccagg atttcccctt tttttaactg cggcatcgcc
     1261 tcaagggttg cgactgccgg ataagggcag ggttcaccca ccatatcaag gcggtaatca
     1321 ggaacgatat ttttcatgcg atctccttag cagtctgcgg tgccgcacgg cggaagaagc
     1381 gtttttccca gccgataatc aacatcagcg cagtaaacaa cagcaaatat gtcaccagca
     1441 ggccacccat cgggccaaag gttttcagca ggttgatttt gtcccagtcg gtggccagcg
     1501 ccggagcgaa atcatcccag taatacgcca gaatcgtcga gccgatcaca ttgcccagac
     1561 cgacccacca gtagtgcacc tggccttcta ccgcgcggta catccagccg gtttcgcagc
     1621 cgcctgccag cacgatgcca aaaccaaaca gtaaaccacc aattaccgcg tttggtcccg
     1681 cccacatgat tttgggttca acgcctaact gtacgtaact gaagatcccg atggcactca
     1741 ccgccatacc gataatgatt gctttcgcca tatgggtacg tccggtgatc cacatatcgc
     1801 ggaacgccga agtaaagcag atttgcgcac gttcaatcag taaaccaaag ccgacgccaa
     1861 acagcattgc cagccccagt ttgggttggt tcatcgccgt gagcagcgcc cagcccagca
     1921 tgccgaaaaa gaccagcatc ccgagacgaa aacgacgccg cgcctgatcc ggtttttgcg
     1981 tcagcggtga ggcggcagaa actttctgca ttttcacggg aatacggaag atgggcagca
     2041 gggtaaagcg cgcgccaaac catgaaccaa tggcagtggc gatggcaaag aaccaggcat
     2101 gcagcgagaa ctgaggaatg ccggtaaaga acgccgccag gttacagccc attgccagac
     2161 gcgcgccaaa accggcgata atgccgccaa taatggcctg cataatgcgg atacggctgc
     2221 gcggcatgcg cagtttgaca ttgttggccc acagcgctgc ggcaaagcag ccgccaaaca
     2281 tgccgaggat catcatcccg tcgatgcggg ttaatggcga tccttccaga tggataattt
     2341 taaaataacc ccactcttca gcatggacgc caaacagctg caggagctga ccgccccaac
     2401 gggtaaattc acccgtgaca gcccaaaagg tgccagtaat gccaaaatag taagtggaga
     2461 gaatacccgc cgcgatgacc gcagggatgg gtgcccagaa tttaatcaac caggcgtgtt
     2521 tgaattgctg ccatgacatg aatgtagcct ctgaactcag aaggtttgaa acaagaatcg
     2581 cgaatcatac atcatgcaaa tttattttcg gggtaataat tcacaaaaat aatagttaga
     2641 tcaaatttct ctctgaacct ggaaaggctg gcgacatcag ggctttaatg ccacaatcct
     2701 cttttcttct catgcaggac gaatgatgaa aaaactcgca attgcaggtg cgctcatgtt
     2761 gctggcgggc tgtgccgaag tcgaaaacta taacaatgtc gtgaaaaccc ccgcgccgga
     2821 ctggctggcg ggctactggc agaccaaagg gccgcaacgt gcgctggtca gtccggaaga
     2881 gataggcagc ctgattgtca ctaaagaggg cgataccctg gattgccgtc agtggcagcg
     2941 ggtgattgcc gtgccgggca agttgacatt gatgtctgat gatctcacta acgttaccgt
     3001 aaaacgtgag ctgtacgagg ttgagcgtga cggtaacacc attgagtacg atggtatgac
     3061 gatggagcgt gtggatcgcc cgacggcaga gtgtgccgcc gcgctggata aagcgccgtt
     3121 accgacgcca ctgccgtaat gaaatctccc cggcttgccg ccggggatga atttaaatca
     3181 cctcttcgat aacccacacg ctgacgctgc cgccgttgca aaagaaggtc gcttcgccgt
     3241 tttcgtcggt cactacgctc tcttgccgat tccccagaaa atcacgccag gttttattgc
     3301 cgtaattctc tcccagatga atggtttttt cgccatcatc cccgttcgac atgaccacca
     3361 cgcagccggg atattcgtca gtgccactgc ggctaaaggc aatgcagttc ggatggtcga
     3421 aaaataacgt ctgtacaccg tgggcgaaac gctgacgggc gagaattaac tcatcaagct
     3481 gttcgattat tggcatatct atcggatagg tttgcccgtc accaccgaca tcttcgtaat
     3541 gcgcaccgta gaggtccgga tagaacaccg aaggaacgcc attttcccgc aacaaaatta
     3601 gagcatacgc cagcggttta aaccacggtt cgaccggcgc ttcgagggct tgcaacggtt
     3661 gggtgtcgtg attggctacg agggttacgg cgtggaaagg atcggcttcc actaatgtac
     3721 ccgtgaaaat ctgcgtcatg tcgtagtcgc gtcccatgcg cgatgcttca tggaatttca
     3781 tctgcagcgg cgcatcgaac agcatggttt tgccttccac ctgatcaata tacgtttgca
     3841 gtttatcgac ttcatgcgac cagtactctg ccacaataaa cagcggcttt ggcgcaactt
     3901 cctgtacgtg ttcgatccac tctttataaa accacgccgg aatatgttta accgcatcaa
     3961 gacgaaaacc gtcgcattgt gtttgttcca tcacccagcg cgcccagtat ttaatctctt
     4021 ccgtcacggc atgattgcgg aaatcgatat tctcacccat cagataatcg aaattaccga
     4081 gttcatcatc aacctgatcg ttccagcctt cgccggtgta gtcgttaaca attttaaata
     4141 tgccatcttc atcaggattt tcgatatggt cgataccgct aaaacatttg aagtcccaga
     4201 taaactgcga gtattgcccg gcacgggcgg ggaaggtgta acgcgtccag ccttcgcact
     4261 caatgatttc ttcatcaatt tgcgtacggt catcagcatt tacacgctgc acgcgaatag
     4321 cttctttttc atccgccccc attttgtggt tgactaccac gtccagtagc accgcaatgt
     4381 cattacgttt cagagcgtca atggcggcca gcagttgtgc tttatcgcca tatttagtag
     4441 ggatgctgcc tttctgatca aactcaccta aatcaaataa atcataggag tcgtagccga
     4501 ccgaataccc gcccgatgcg cctttatagg cgggcggcaa ccagaccata ttgataccaa
     4561 tatcattaaa accgtcggcg cgctcggcca gttcaggcca gagcttaccg ccttccgggt
     4621 aataccagtg aaaacattgt aacagcgtgg gattacgcat tcttcgactc cattcaaggg
     4681 gaacattaga agcgtagccg taatcggatt attcgcgagc catcggctcg ttaagattca
     4741 aaagaggtta tcctgcggag ccagcacaaa gccgccctga tctccatagg cacttaacac
     4801 tgatttttgc accgatgact gaccgaccag tttcgccagt tcatccatac gaatctgtaa
     4861 taactgcttt accttgcttt cgttgtcgag aatcaggcgc agcatcgggc ggagctgctc
     4921 ctgcatcgtg gtggacgggg caacctcttc agtcagatgt gcaatctcct gcaccgcatt
     4981 cacatacgcc atttcgctgg cgatgagttc gtcccattgt tcttccgttg ccaggcgtaa
     5041 catgagctgg cttttatcga cgagttgttg ccaggcgaaa tacaaatgcg gtgcatggtt
     5101 catcagactg ggtcctgaat caaagaaggg gagagtaaag actctttcca ggcatcggca
     5161 atattgcgca ttaatgcttc cacttcttcg actgcggaga catcgttgcg taaattggct
     5221 tgcagcaagc gcctgaccat atagctataa agagcaatca agttttgggt tagttcgtct
     5281 ttgctctctt catcaagact cacccgcagc ccgttctcaa tgatgttgat cgcttttgac
     5341 aaagagacgc ctttgccttg ctgattgttg tcctgcataa acaggcgcgc tctaaccagt
     5401 gcgctcagca ctccatcaaa tagcatggtg accagctgtt gctggctggc gctcattacg
     5461 gcgctttcga cgccaatttg tgcataggct tttgagccgc tcgcggtata catgctgacc
     5521 tccgtgatca ggaattactc attgcagtaa attgttggct gagataggta ctggtgttat
     5581 tcagtttgct catcatggta tctaattgcg taaactgtgc agtgtaacgc gccacggtgt
     5641 catcaatgct ggcactgacg gatagatact gtttggtcag ttttttcagt gtggcgttaa
     5701 tactgtcctg tgcgctatca ataatgccat catcggcaag gtaacctttt acctcggtgg
     5761 caattttggt ggtaattcct gtttctttac catcgcctac cagcaattca cgtacggatg
     5821 ctgtattttc attcaggact tttttgagtt tatcatcatc aattttcagc ttacccgtcg
     5881 tgccgtcctg cttaatcccg atctcgttta atgttttgaa ggcactgtcg ctggcaccgt
     5941 ttgcaaattg tgcgcggatg ccgctttgaa ttgtacgtac aacgctgtct ccgagaagtg
     6001 caccattatt tttatcttgt gcttcagcac ctgggtctac ttctttat
//
LOCUS       DAJVRF010000189         6015 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJVRF010000189 DAJVRF010000000
VERSION     DAJVRF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.189"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(369..1304)
                     /locus_tag="OQN13_003671"
     CDS             complement(369..1304)
                     /locus_tag="OQN13_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCS1616923.1"
                     /translation="MSAALQYFEENLPLRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDLNAPAPNITIKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAIERALCEKLGADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLS
                     TSARRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTAETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
     gene            complement(1802..1945)
                     /locus_tag="OQN13_003672"
     CDS             complement(1802..1945)
                     /locus_tag="OQN13_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HCS1616924.1"
                     /translation="MKKITGIILLLLAAIILAACQANYIRDVQGGTVSPSSSAELTGV
                     ETQ"
     gene            complement(2029..2286)
                     /locus_tag="OQN13_003673"
     CDS             complement(2029..2286)
                     /locus_tag="OQN13_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="HCS1616925.1"
                     /translation="MGLKLHIHWFDKKNEEFKGGEYSKDFGDDGSVIERLGMPLKDNI
                     NNGWFDVEKRWVPILQPYFKNVIDISKFDYFVSFDYRDGDW"
     gene            complement(2296..3933)
                     /locus_tag="OQN13_003674"
     CDS             complement(2296..3933)
                     /locus_tag="OQN13_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="HCS1616926.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENN
                     PWGGGSGSGVHWGGGSGNGNGGGNGNSGGGSNSSVAAVAFGFPALATPGAGTLGIAVS
                     GEALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTN
                     VQVSTLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASF
                     PGVPSLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKP
                     VYVSVTDVLTPAQVKQRQDEEKRLQQEWNDAHPVEVAERNYEQARAELNQANKDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQ
                     RAQTDVNNKKAAFDAAAKEKSAADAALSSAMESRKKKEDKKRSAENKLNEEKNKRRKG
                     TKDYGHDYHPAPKTEDIKGLGELKEGRPKTPKQGGGGKRARWYGDKGRKIYEWDSQHG
                     ELEGYRASDGQHLGSFDPKTGKQLKGPDPKRSIKKYL"
     gene            complement(4154..4687)
                     /locus_tag="OQN13_003675"
     CDS             complement(4154..4687)
                     /locus_tag="OQN13_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616927.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQRLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFSVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(4690..5616)
                     /locus_tag="OQN13_003676"
     CDS             complement(4690..5616)
                     /locus_tag="OQN13_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCS1616928.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQNRTEPERVKSPSRGFSMGF"
     gene            5792..>6015
                     /locus_tag="OQN13_003677"
     CDS             5792..>6015
                     /locus_tag="OQN13_003677"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616929.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQER
                     KLLHVLATAEKNDLQAQESERKQAVQKRIEG"
BASE COUNT         1487 a         1660 c         1297 g         1571 t
ORIGIN      
        1 aaaaaaacaa cgacgagaac gcaatcaccg actctttctc gcggctgggc tactgatcga
       61 agcaggactg gtagacacaa aaacaggcga gctatgctat aaaaaagaca ggatattaca
      121 ggcacttaag gaaattaagt acgatctgga gacatcccca aacccagacg cgtaagtgcc
      181 tccatccgtg ttcctgttcc ctggtgcttc ctcgctcatt cgctcgctac gctcggtcat
      241 gagactgcgg cgagcactac cagcacaatc atgcggaaaa cagcaccatt gcagggctga
      301 acggggaaaa acggcaaaag acctaaggct caaaaaaacg ccagaaaggc gctgttatct
      361 gatataggct attttccgct tttccagttt cgaatggttc gagtagaaac ctccaacatc
      421 gccgcaatag ctttctgaga atatccattc tccagcatac atagcgccat gaaacgcttg
      481 tcatcgtagg cttcgccttt agcgatgcca cctttcctgc ctctcttggc ctgtatttct
      541 ggcgtgtgcg tatcagccac atactgtgcg aaagtttccg ccgtgaagtt cctgtgcgtg
      601 tatttcgcaa tactcttgcc aatagcccga cattcaggag gtgaaagcgg aacgggaagc
      661 gatgcgttgt acatttcgac acgctgaatc acggcatcaa gccactgtga gaatgctggc
      721 cagccctgac gaatcgccct gtaagcccat ttgcgcgtca tttcgaacag atggcagttt
      781 ctccccattc cgtaatgttt atcgatgcta cggcgcgctg aagtgctcaa atcgagataa
      841 tcagccagtt catcgagggt ataggcttcc tcacgccact ccatcacctg ccattccaga
      901 tggaacggat ttttgcaaat cagtccgctg tagtttacat ctgcgcccag tttttcacac
      961 aacgcacgtt caatcgcggc ggcgtatttc agcgccttaa ccgaagcatc aggcgcggtt
     1021 ctcacggcga tattgagtgc gtagagcagg tgggcgtgac cgttcacggg attttttatg
     1081 gtgatgttcg gtgcgggggc gttcaggtcg ctccagtcaa tcgcagcccc ctcgcggtca
     1141 acgtcaaaaa ccagccagaa ctgcgcatga ggttggttct gctggatata ccgtgcaaga
     1201 agcgcgcgcc ctttaccgga gatacgaagg ccgaaagcga gatcatccgt gtgatagggg
     1261 cggaggggta aattttcttc gaagtattga agcgcggcgc tcatgtgtta cgattccttc
     1321 caggttatcg accgacctcg cccctgtatt ctcgtgacaa tcttggcgga gagacacgaa
     1381 tttcaggggc gtttgttttt cagtcgctta cactacaccc aacggcaaat cctgtcgatc
     1441 cttcccgaaa acccgatcca tttaccataa ctcccgttgt acgtacccct taaaacgccc
     1501 tcaatcgctt tctggcgcaa aaaacgcaaa aagacaagaa aacaccagga caaaccagaa
     1561 aaacgcgtca gaatcgtttt cagggcgttg cataactatg cacgaaagcg atgtgcgatc
     1621 ggtttcgtgc gttctgaaca ccgaacggct caaaacgaaa agttttctcc ctccggatgg
     1681 aaggttctgc acggagattt tagatcccat ctcccacatg gaatttttgt ggcacttcaa
     1741 caaaaaaagc gcgtcagaat cagcctgaca cgctttgtcc ttgtcaaatg aggattgcag
     1801 gttactgcgt ttccactccg gtcagttcag cagaggatga cggtgataca gtcccgccct
     1861 gaacatcacg gatatagttt gcctgacatg cagcaagaat aatggctgca agaagcaaca
     1921 aaataatccc tgttattttc ttcatacgat tccctgtata tatggattca ggaattcatc
     1981 cttgaacacc tgaatcaaac actttattac tcgtccctga tagtttaatt accagtcacc
     2041 atcacgataa tcgaaagaaa caaagtaatc gaacttacta atatcaataa catttttaaa
     2101 gtatggctgt aatattggca cccatctctt ttcaacatca aaccaaccgt tattaatatt
     2161 atcctttaaa ggcattccaa gacgttcaat aaccgaacca tcatcaccga aatcctttga
     2221 atactcacca cctttaaatt cctcattctt cttatcaaac caatgaatgt gtaatttaag
     2281 tcccataaca tcaccttaaa gatacttttt aatacttcgt ttcggatctg gacctttcaa
     2341 ctgcttaccg gttttagggt caaatgaacc aagatgctga ccatcactgg ctcgatagcc
     2401 ctcaagctca ccatgctgag agtcccactc ataaatttta cgccccttat ctccatacca
     2461 tctggcacgc ttacctccac caccttgctt tggagttttt ggcctcccct cttttaattc
     2521 ccccaaacct ttaatatcct cagttttagg agctggatga taatcatggc cataatcttt
     2581 agtgcctttt cggcgtttat ttttctcttc gtttaattta ttttcagcac tccttttctt
     2641 atcttctttc ttcttcctgc tttccatcgc agaactcaat gcagcatctg ctgctgactt
     2701 ctcttttgcg gcagcatcaa atgcagcctt cttattattt acatccgtct gtgcccgctg
     2761 ggccttaagc cctgccattt gccacattct gtgaccagca gccattggtt ctcgggcaaa
     2821 tcgctcgaat tgttttattt cagcctttgc atcagcaaga gttttattcg ctgcatcaag
     2881 ttcactttta cgagaattat aaacctgaac agctttagcc tgtcgttcct ggtttctggc
     2941 aacatcttta ttagcctgat tcagttctgc gcgggcctgt tcataattgc gttcagccac
     3001 ttccaccgga tgtgcgtcat tccattcctg ctggaggcgt ttttcctcat cctgacgttg
     3061 ttttacctgt gctggggtaa gaacatctgt cactgataca taaaccggct tctgcccact
     3121 ttctttcgga aagcgaatca ccgcttcatg tgaaccacca ccaaaggtaa atcccgcagg
     3181 aacggcagtc cggcctttat cctccgtaat accacgggga agggttgttg acaccggaag
     3241 gcctttgaca gtgctgactg tcagagaagg gacaccagga aatgatgcgt gaaatacacc
     3301 tggagtacgt gttggtttgg cattcacaac aggcacactc ataggcacac ctgcaactac
     3361 ggcaatatgc tgtcgtgtgt ccttcacaac atctgtcaca cgtttcgtca cgctgacagt
     3421 tgcctggtcc agcggaagtg tactgacctg aacattcgtc actgtttctg ccggtaatga
     3481 cgtcacaatc tttgacatca tattcgggtc atcttttgct atttcagatg gcagaatgcc
     3541 gtaaagcgca ataccccatg cgctgaattt aaacgggcct ttcagggctg caaagatatc
     3601 cgctattgcc gcagataaag cctcaccaga gacggcgata cccagcgttc cggcaccagg
     3661 agttgccaaa gcaggaaaac caaatgccac tgctgctacg gatgaattgc tgccaccacc
     3721 ggaattacca ttccccccgc cattaccatt gccggagcca cctccccagt gaacaccact
     3781 accggaaccg ccaccccatg ggttattttc cgaactccat cctgaaccgt cagaagcccc
     3841 accatttcca ccaagccccg tagggccgcc attaatgtta ccacctgtgt tatgtgcgcc
     3901 actgttgtgg tcgcgtccat ctccaccgct cataaaaatt ccctctttta agcgttaaaa
     3961 caattgatta tatagttgat actgtacata aaaccactgg ttttatgtac agaccaaaaa
     4021 gtactcgcca ttattttttc tgtcaagtca gggggggata ttttttggca gttcggagga
     4081 atcactcatg aaaacggtga acggaacgcg aaatcctttc gcgggtcgtg aggcgttgga
     4141 caagcgaagc gcgtcagttt ttccggctct tacccgtgac aggtttctcc accagtttat
     4201 cttccagtgt cacaactttc tgatacaccg ccgctgtggc gttgtagttc ctgtcgagaa
     4261 ttgttttcat cccccatccg agcacactga acataaccag aaaggccact gacaccccac
     4321 ccagccattt ccagctatac cctttaaggg tgttgtattc ctgcacgata ttgctgtgaa
     4381 ttgtcgcaat atccctgttc agggcatccg cgtttgcctt catgtttgcg actgactctt
     4441 ccagccgctg aaaatcctga ctgatgcgct cacctatcag ccctgcaaca tccagcctct
     4501 gcacttcgtt tctgacttca tccctgacct gttttgccac accgtcagaa atatcccgat
     4561 atccgcgaag tgtttccctt gccacagcca gagaatcagc aacagctttc tgttgctcca
     4621 ttgcggtatg ttgcagatgc tccaccgata acaggagcgt gagtaatttt tcttctgtgc
     4681 tggccatgtt cagaatccca tagaaaatcc acgtgacggt gatttaaccc gttcaggttc
     4741 agttcggttc tgtctgctca ctctgtctct catgtcaaag ccatgctccc gcgcaacatc
     4801 atgttgcagc cttctgacca gttcatgttg cttaaccgga gtgtccagcc attccctgat
     4861 gtcatgcccg accgttcgcg cctgttcaag atggcgggaa tagtccggtt cagaccgttt
     4921 ttgcatgatg tcatgacgct gggcatcatc aagccgtccg gcagcttcaa aacccagatg
     4981 cacttcggga cgtcgttcaa tgccctgttc tttcagcgtc cggcagtcaa tgaagcccct
     5041 ttcaaagcca tgctttctca tatgttcgtt gtgcttcacc tcccagcgtt ttcgcaattc
     5101 ccgcaactgc tctttttgct gagtgagagt caggcttccg gaatcctttt tcgcacctcc
     5161 ccgctcggga tacctggcgt tatagcgccg gaaatactgt tccggtgtac gttcaatgcc
     5221 atcattcaca cgctggctca tcatgatatg cgcatgaggc tgttcgccgc cgtcaatgct
     5281 cgctttcgga ttgtgaatgg caaaactcca ggcgtgccgt tcgccagctt cctgacgtac
     5341 aaaatcctgc accagttcaa ggcgctgttc cgctgtcagt tcacggggta atgcaatttc
     5401 aagttcccga taggtcgaac cgttcgcacg ttcaaactgg tcagccgcct gccagaaatg
     5461 tgagggatta tctcgcgccc attccggcat gtttccgtgt gcggaatatt ccaaatcctg
     5521 acggtcgcgg tatttctctt tgcgctcgat gtaatcggcg tggtttgcag cctgcccctt
     5581 gccaccaaat ttaacgctga ggtgataggt tgccacggtt tcagccctcc gcaggagcgc
     5641 actgtttctc gacgaaggag agaaacatat aagtgcgctt ccttctaatt ttacgaatta
     5701 gcacggaaga taccccctgc acccaccgac cgcaagcgat atactgcacg accacaacac
     5761 acagcaaaaa acaccaggga gcaccacgaa catgcgcatc agcaacatcg aatggctgaa
     5821 gaaacggatc ggattcatca ggaagctcgg agaacagacc gcacgacaga gacagattat
     5881 tgacctgctc gacaacgaag caggactcac agaacaggaa cgcaaactcc tccacgtact
     5941 ggcaacagcg gaaaaaaacg acttacaggc acaggagagc gaacgaaaac aggcggtcca
     6001 aaaacgaatc gaggg
//
LOCUS       DAJVRF010000190         5977 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJVRF010000190 DAJVRF010000000
VERSION     DAJVRF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.190"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..354)
                     /locus_tag="OQN13_003678"
     CDS             complement(<1..354)
                     /locus_tag="OQN13_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCS1616930.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAK"
     gene            complement(495..2528)
                     /gene="metG"
                     /locus_tag="OQN13_003679"
     CDS             complement(495..2528)
                     /gene="metG"
                     /locus_tag="OQN13_003679"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCS1616931.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2660..3769
                     /gene="apbC"
                     /locus_tag="OQN13_003680"
     CDS             2660..3769
                     /gene="apbC"
                     /locus_tag="OQN13_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCS1616932.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4014..4295
                     /locus_tag="OQN13_003681"
     CDS             4014..4295
                     /locus_tag="OQN13_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCS1616933.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4538..5080
                     /locus_tag="OQN13_003682"
     CDS             4538..5080
                     /locus_tag="OQN13_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1616934.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5160..5834
                     /locus_tag="OQN13_003683"
     CDS             5160..5834
                     /locus_tag="OQN13_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS1616935.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
BASE COUNT         1576 a         1370 c         1430 g         1601 t
ORIGIN      
        1 tttcgccagc caaggtaaaa cagcatctgt atttttgtca ggcgcacggg tttctgctac
       61 gaaagagggt tgttcctgaa taatggtgga taagtccgct accttgagag agcccgttac
      121 cgtttgggaa ggttgcaaag aattatcctt cgcttgctcc acatacccct tcttcacctt
      181 ctccgcaatc agcttaagct ccgcttttgc cgctgccgca gcatctgaaa aactttttat
      241 ctggctttgc cctttggtgc caacttttcc ccaactgata tgcaactcgt ttccctgttg
      301 ctcaactgcc cagaatttat gtgatttttc gtcctgatag ataaagtgtc tcattctgca
      361 tccctgtaat tttatccagt tgaatgcaga tgctaccagt atttatgcgg gttagagaga
      421 gacaaaatcg caagaaaaca actttaggaa tttataaaga aaaggcgctg tcgatgcagc
      481 gccttgaagg gggattattt cacctgatgg cctggtttag caccagcatc cgggcttaac
      541 aggaagatat ctttcccgcc aggaccagca gccattacca tgccttcgga gataccaaag
      601 cgcattttac gcggtgccag gttggccacc ataatggtgt gacgcccgat cagtgcctgc
      661 ggatccgggt aggcagaacg aataccggag aagacattgc gtttttcacc gccgagatcc
      721 agtgtcaggc gcagcagttt gtcagaacct tcaacaaact ctgcgttttc gatcagcgcc
      781 acgcgcaggt caacttttgc gaagtcgtca aaggtgatgg tttcctgaat cggatcatct
      841 gccagtgggc cagtaactgg cgcggcagct gctttcactt cttctttaga cgcttccacc
      901 agtgcttcaa cctgcttcat atcgatgcgg ttatacagcg ctttgaacgg attcactttg
      961 tggcccagca gcggttgctg gataccatcc caggtcagtt ctgtattgag gaatgcttct
     1021 gcgcgctcgg tcagtttcgg cagtaccggc ttcaggtaag tcatcagcac gcggaacagg
     1081 tttatgccca ttgagcaaat tgcctgcagg tcggcatcgc ggccttcctg ttttgccacc
     1141 acccacggag cctgctcgtc gacatagcgg ttagccagat cagccagcgc catgatttcg
     1201 cgcactgctt taccaaattc gcggctttcc cacgcttcac caatcacttc agcggcatca
     1261 gtgaaggttt tgtacaactg cgggtcagcc agttcgcttg ccagcacgcc gtcaaaacgc
     1321 ttgttgataa agcccgcatt acgagaggcc aggttaacca ctttgttcac gatatcggca
     1381 ttcacacgct gaacgaaatc ctccaggttg agatcgatat catcaatgcg cgaagagagt
     1441 ttcgcagtgt agtagtaacg caggctgtca gcgtcaaaat gattcagcca ggtgctggct
     1501 ttaataaagg tgccgcgaga cttggacatc tttgcgccgt tcaccgtcac atagccatga
     1561 acaaacaggt tggtcggctt gcggaagttg ctgccttcca gcatggcagg ccagaacagg
     1621 ctgtggaagt aaacaatatc tttaccgatg aagtggtaca gctcggcggt ggagtctttc
     1681 ttccagtatt catcgaagct tacgctgtcg ctgcgcttgt cgcacagatt cttgaaagaa
     1741 cccatgtagc caatcggtgc gtccagccag acgtagaaat atttgcccgg cgcgttcgga
     1801 atttcaaaac caaagtatgg cgcgtcgcgg gagatatccc actgttgcag gccggattca
     1861 aaccactcct gcattttatt tgccacctgc tcctgcaacg caccgctgcg ggtccatgcc
     1921 tgcaacattt cgctgaaaga gggcagatca aagaagaagt gttcagaatc acgcattacc
     1981 ggcgtagcgc cagaaaccac cgatttcggc tcgatcaatt cagtcgggct gtaggtcgcg
     2041 ccgcagactt cgcagttatc gccgtattga tccggggatt tacatttcgg gcaggtgcct
     2101 ttcacaaaac ggtccggcag gaacatgcct ttttccggat cgtacagctg agagatggtg
     2161 cggtttttaa taaaaccgtt ctctttcagg cgagagtaga taagttctga caactggcgg
     2221 ttctcttcgc tgtgcgtcga gtgatagttg tcatagctga tgttaaagcc tgcgaaatca
     2281 gtctgatgct cctgactcat ttcgccaatc atctgctccg gggtgatacc aagctgctga
     2341 gctttcagca tgatcggtgt accgtgggca tcgtcggcgc agatgaagtt gacctcgtgg
     2401 ccgcgcattc gctggtaacg gacccagaca tcagcctgga tgtgctccag catatggccg
     2461 aggtggattg agccgttagc gtacggcagt gcgcacgtca ccagaatttt cttcgcgact
     2521 tgagtcatag taggcattac ttcttaatag tgaaaagggg ttagatagta ccaaatggga
     2581 aaatgttaag taagtacttc tctatattct tcaggtaaac tatcagctga tggtttatct
     2641 atacaaaaaa ggagtcggga tgaacgaaca atcccaggcc aaatcaccag aagccttgcg
     2701 cgcaatggtc gccgggacac tggccaattt tcagcaccca accctgaagc ataacctcac
     2761 cacgctgaac gcgttgcacc atgtcgcctg gatggacgac acgttgcatg tggaactggt
     2821 tatgccattc gtctggcata gcgcgtttga agagttaaaa gagcaatgta gcgccgaact
     2881 gctgcgtatc accggggcga aagctatcga ctggaaactg tcgcacaata tcgccacctt
     2941 aaaacgcgtg aagaatcagc cgggtattaa tggtgtgaag aacatcattg cggtcagttc
     3001 cggtaaaggc ggcgtgggta aatcctccac ggcggtaaac ctggcgctgg cactggcggc
     3061 ggaaggggcg aaagtcggca ttctggacgc cgatatctac ggcccatcaa ttcccaccat
     3121 gctgggcgca gaaaatcagc gtccaacttc gccggacggt acccatatgg caccgattat
     3181 gtctcatggc ctggcaacca attcaattgg ttatctggtc actgacgaca atgcgatggt
     3241 gtggcgtgga ccgatggcga gcaaggcgtt aatgcagatg ttgcaggaaa ccttgtggcc
     3301 ggatctcgac tatctggtgc ttgatatgcc gccaggcacc ggtgacattc aattgacgct
     3361 ggcgcaaaac attccagtaa ctggtgcagt cgtggtcacc accccgcagg acatcgcttt
     3421 gatcgatgcg aaaaaaggca ttgtgatgtt cgagaaagtc gaggttccgg tgcttggtat
     3481 tgtcgagaac atgagtgtgc atatttgcag taactgtggt caccatgaac cgattttcgg
     3541 taccggtggt gcagagaaac tggctgaaaa ataccacaca cagctgctgg ggcagatgcc
     3601 gttacacatc tccttacgtg aggatctcga taaaggcacg ccaacggtta tcagccgtcc
     3661 ggagagcgaa tttaccgcta tctaccgcca gctggctgac cgcgttgcag ctcagctcta
     3721 ctggcagggt gaagtcattc caggcgagat ttccttccgc gcggtctaac gcctattaac
     3781 cattctggtt attcttcata caccgcagag atgttacatt gatgcggtgt attagttttt
     3841 gccgcattaa tgaacactta tttaatactg cgaatattct gcggaccttc gtccgaaact
     3901 ctacaaagtc gattgatcat acaggcaatg cttcattatc atccttcgtg gctgtgtagt
     3961 ggatgtacag ctatagtttg ttatgcactc attctatcca ggcataacct tcaatgaata
     4021 agtattggct gtcaggaatt atttttcttg cctatggatt ggcttctccc gcattctctt
     4081 cagagactgc cacattagcg attaatggca ggatcagtcc cccaacatgc agtatggcta
     4141 tggttaatgg tcaacctcaa ccgcattgtg gtcagttaac gtataatgtt gacacccgtc
     4201 atctgttttc ttcgcccgtc aaaggtgtaa cgacagaagt ggttgttgca ggtagcgata
     4261 gcaaacgtcg aatcgtgtta aatcgctacg attaatttgg caacctgatt tttattgata
     4321 tcaacaatac catttacata aatctacatt aattgataga tgcgataaat agttaatact
     4381 cttaaatatt tgttgtattt atattgcaat ggttaatttg tttcaaataa ataaaacaat
     4441 gtgtttacat tgcggtaact tttttgatgt gagaaatttg tttccaagag atacagactc
     4501 ttaacaacca aaaaaaaatt ttattaagga acatattatg aaacgttcaa ttattgctgc
     4561 cgctgtcttt tcttcttttt ttatgagcgc tggagtattt gctgcagacg ttgataccgg
     4621 aacattaact attaagggga atattgcaga atctccgtgt aaattcgaag cgggtggtga
     4681 ttcagtaagt attaatatgc cgactgtacc aaccactgtc tttgaaggta aagctaaata
     4741 ttctacctat gatgatgcag tcggtgtaac cagcagcatg ttaaaaatta gctgcccgaa
     4801 agaagttgct ggtgtaaaac tctcgttgat taccaacgat aaaataaccg gtaacgataa
     4861 ggcgatagcc agtagcaacg ataccgtggg ttactatctc tatttaggtg ataacagcga
     4921 tgtcctggat gtttctgcac cttttaacat tgagagttat aaaacagcgg aaggtcaata
     4981 tgctattccg tttaaagcaa aatacctgaa actgacagat aactcagtgc aatcaggtga
     5041 tgtgttatct tctctggtta tgcgtgtggc gcaggattaa taaataaaat tgtaagtttc
     5101 tccaggtaat aaacatggcc gctatcccat ggcggccttt taatttaaga ggcattaaaa
     5161 tgaaaggatt attatcttta ctcatttttt ctatggtcct tcctgcacat gccggaattg
     5221 ttatctacgg gacgcgcatt atttacccgg cagaaaataa agaagtgatg gtgcagttga
     5281 tgaaccaggg aaaccgttct tcgctgctgc aggcgtggat tgatgatggc gatacgtcat
     5341 taccaccaga aaaaattcag gttcctttca tgttaacgcc accagtggca aaaatagggg
     5401 caaattccgg gcagcaagta aaaatcaaaa ttatgccgaa taaactgccc actaataaag
     5461 aaagcatttt ttatctgaat gttctggata ttccaccaaa tagtccagag caagaaggta
     5521 agaatgcact gaagtttgcg atgcaaaaca gaattaagtt gttttaccga ccagcgggta
     5581 ttgctccggt aaataaagcg acatttaaaa aattgctggt aaatcgcagt ggcaatggtt
     5641 tggtgataaa aaatgactca gctaattggg tgacgatttc ggatgtcaaa gctaataatg
     5701 tcaaagtcaa ttatgaaact attatgattg cccccttaga aagtcagagt gttaatgtca
     5761 aaagtaataa tgcaaataac tggcatctga ccattatcga tgaccatggc aactatatta
     5821 gtgacaaaat ttaattgcag gagctgccta tgttgagaat gaccccactt gcatcagcaa
     5881 tcgtagcgtt attgctcggc attgaagctt atgcagctga agaaaccttt gatacccatt
     5941 ttatgatagg tggaatggaa gaccagcagg ttgcaaa
//
LOCUS       DAJVRF010000191         5939 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJVRF010000191 DAJVRF010000000
VERSION     DAJVRF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.191"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            79..429
                     /gene="traA"
                     /locus_tag="OQN13_003684"
                     /pseudo
     CDS             79..429
                     /gene="traA"
                     /locus_tag="OQN13_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            444..755
                     /gene="traL"
                     /locus_tag="OQN13_003685"
     CDS             444..755
                     /gene="traL"
                     /locus_tag="OQN13_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HCS1616936.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            777..959
                     /locus_tag="OQN13_003686"
                     /pseudo
     CDS             777..959
                     /locus_tag="OQN13_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            961..2211
                     /locus_tag="OQN13_003687"
                     /pseudo
     CDS             961..2211
                     /locus_tag="OQN13_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            2208..2594
                     /gene="trbI"
                     /locus_tag="OQN13_003688"
     CDS             2208..2594
                     /gene="trbI"
                     /locus_tag="OQN13_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HCS1616937.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            2591..2902
                     /locus_tag="OQN13_003689"
                     /pseudo
     CDS             2591..2902
                     /locus_tag="OQN13_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            2897..3895
                     /locus_tag="OQN13_003690"
                     /pseudo
     CDS             2897..3895
                     /locus_tag="OQN13_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            3915..4662
                     /gene="traX"
                     /locus_tag="OQN13_003691"
                     /pseudo
     CDS             3915..4662
                     /gene="traX"
                     /locus_tag="OQN13_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            4717..5277
                     /locus_tag="OQN13_003692"
     CDS             4717..5277
                     /locus_tag="OQN13_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCS1616938.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            5407..5619
                     /locus_tag="OQN13_003693"
     CDS             5407..5619
                     /locus_tag="OQN13_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCS1616939.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1402 a         1442 c         1810 g         1285 t
ORIGIN      
        1 gcttccatcg gaaacgatga tctggcagcc tgagttcaca gataaaacac tctccaggaa
       61 acccggggcg gttcagcgtt aagtgttcag ggtacttctg cgcccgtcaa aaagaaatcg
      121 tttttttcta aattcactcg tctgaatatg cttcgcctgg ttcgtgcagt gatcccggtt
      181 gctgttctga tgatgttgtt ccctgagtta gctatggctg ctggtaaagg cacggacctg
      241 atggctaaag gtcaggaaac ggttaaggcg accttcggta aggactccag tatcgttaaa
      301 tgggttgttc tggctgaagt tctggtcggt gcagtcatgt acatgatgac caaaaacgtc
      361 aagttcctgg tcggctttgc catactctct gtatttattg cagtaggtat gtctgttgcc
      421 ggattctgac aggaaataaa acgatgttgg gagacgagaa taaacttaag aaatatcgtt
      481 tcccggaaac actgaccaac cagagccgct ggtttggcct gccactggat gaactgatcc
      541 ccgcagcaat ctgtgttggc tggggtatca caacatcgaa atatctgttc ggtattggtg
      601 cagcggttct ggtttatttc gggattaaaa aactgaaaaa agggcggggc agttcctggt
      661 tacgtgacct gatttactgg tatatgccaa cagccctgct gcgcggtatt tttcataatg
      721 ttcccgattc gtgtttccgg cagtggatta aatagaactg ataccaggat tgttatatgg
      781 aacacggtgc ccgtttaagt accagtcgtg taatggccat cgcctttata tttatgtcag
      841 tgcttattgt tctcagcctc tctgttaacc tcattcaggg ggtgaataac taccgtcttc
      901 agaatgagca acgcactgcc gtgaccccaa tgggatttaa tgtgcccttt gccgtgtcac
      961 acctaccgta accagctggc gtttatcgat attttcttcc ggggaatgaa taacaccaac
     1021 tacaacatgg cggtctgtgg cacctccggg gccggtaaaa ccgggctgat acagccactt
     1081 atccgcagcg tgctggactc cgggggcttt gccgtggtgt tcgacatggg ggatggctac
     1141 aagtccctgt gtgagaacat gggtggcgta tatctggacg gtgaaaccct gcgttttaac
     1201 ccgtttgcga acatcaccga tatcgaccag tcagcggagc gtgtccgtga ccagttgtca
     1261 gtgatggcca gccccaacgg taacctggat gaagtgcatg aaggtctgct gctgcaggcg
     1321 gtcagggcct cctggctggc caaagagaac agggcacgta ttgatgacgt ggtggatttc
     1381 ctgaaaaacg ccagtgacag cgagcagtat gccgggtcac cgactatccg cagccgtctg
     1441 gacgaaatga ttgtgctgct tgaccagtac actgccaacg gcacttacgg ccagtatttt
     1501 aactctgatg agccgtccct gcgggatgac gcaaaaatgg tggtgctgga gctgggcgga
     1561 ctggaagacc gtccgtcact gctggttgcg gtgatgttct ccctgattat ctacatcgag
     1621 aacaggatgt accgcacgcc gcgtaacctc aagaaactga acgtcattga tgaaggctgg
     1681 cgtctgctgg acttcaaaaa tcacaaggtc ggtgagttta ttgagaaagg ttaccgtacc
     1741 gcccgtcgtc ataccggtgc ttatatcacc atcacacaga acatcgtcga ctttgactct
     1801 gacaaggcct ccagtgcggc ccgtgcggca tggggtaact cctcctacaa aattatcctc
     1861 aaacagagtg cgaaggagtt cgcaaaatac aaccagctgt atccggacca gttccagcca
     1921 ctgcagcgcg acatgattgg taagtttggt gcggccaaag accagtggtt cagttccttc
     1981 ctgctgcagg tggaaaacca ttcctcctgg caccgcctgt ttgtggaccc gttaagccgc
     2041 gccatgtaca gctctgacgg cccggatttt gagtttgtgc agcagaaacg taaagagggg
     2101 ctgagtattc atgaggcggt gtggcagctg gcgtggaaga agtcagggcc ggagatggct
     2161 tcgctggaag cctggctgga agaacatgag aaatacagga gtgttgcatg accacaacgc
     2221 aaaaaacgac tgatgttaca gccccccgac gcagccactg gtggtggacg gtgccgggat
     2281 gcctggcaat ggtgttactg aacgcagccg tcagttacgg cattgtcaga ctgaacgcac
     2341 cgatgactgc cgccttcaac atgaaacaga ctgtggatgc attttttgac agcgccagtc
     2401 agaaacaact gtcagaagca cagtcaaaag ccctttcagc gcgttttaac acggcactgg
     2461 aagccagcct gcaggcgtgg cagcagaaac accacgcggt cattctggtg tcgcctgccg
     2521 tggtacaggg ggctccggat atcacccggg aaatccagca ggatattgcc cggcgaatga
     2581 gagcggaacc atgagatgcc gggggctgat tgccctgctg atatgggggc agagtgtggc
     2641 cgccgccgat cttggcacct ggggtgatct gtggccggta aaggagccgg acatgctgac
     2701 ggtgatcatg cagcgcctga cggcactgga gcagtccggt gagatgggcc ggaaaatgga
     2761 tgcctttaag gagcgggtga tccgcaacag tctgcggccc cctgccgtgc ccggtatcgg
     2821 acgcacggag aaatacagca gccggttgtt tgacccgtcc gtcagactgg ctgcggatat
     2881 ccgggataac gaagggcggg tggccggctt tgagtcagcc tacgtggccc tgtcgcgtat
     2941 gaagcagcat gtgcaggtgt acaccgataa ccgtcagggc tggacggatg ccatcaacaa
     3001 tgccgtacag aaaggaactg cccacgatgt gtttgagccg aaaccggacc gggaggtcat
     3061 gaatgcagag cggctgttca gtacggcgcg ggagctgcgg gacgtggcgg caggccgtgc
     3121 cgttctccgt caggcggggc tggccggggg agacagtcct gcacggttta ttgctccggg
     3181 acgtaaatat ccgcaaccgt atgtggcact gccggcgttt gaccgtaacg gcaagtccgc
     3241 cggtatctgg ctgaacccgc tgaccacgga tgacggaaac gggctgcggg gattcagcgg
     3301 tgaaggtcgg gtaaaaggca gcggggatgc gcagttcgtg gccctgcagg gcagccgtaa
     3361 cggagaaagc ctgctggctg ataatatgca ggatggcgtc cggattgccc gtgataatcc
     3421 tgacagtggt gtggtggtaa gcattgccgg tgaaggccgt ccgtggaatc ccggtgccat
     3481 caccgatggc cgcgtgtggg gggatatccc ggacaacagc gtccagccgg gagccggaaa
     3541 tggcgaaccg gtcacggcag aggtactggc acagcggcag gctgaagagg ccatccgccg
     3601 tgaaacggaa cgtcgcgcag atgaaattgt ccgtaaaatg gcagagaaca aacctgacct
     3661 gccggacggc aaaacagagc tggctgtcag ggaaattgcc gggcaggagc gtgaccgggc
     3721 tgccataact gaacgggaag ccgcgctgcc ggagagtgtg ctgcgtgaat cacaacggga
     3781 gcgggaagcg gtccgggagg ttgcccggga aaatctgctg caggagcgtc tgcagcagat
     3841 ggagcgggat atggttcgtg acctgcagaa agagaaaacc ccgggcggag actgatacag
     3901 gaagacaaac actgatgaca acggataaca cgaacacgac acgtaacgat tcactggctg
     3961 cacggaccga tacctggttg cagtcatttc tggtctggtc acccggacag cgggatatca
     4021 tcaaaacggt ggcactggtg ctgatggtac tggaccacat caatctgata ttccagctga
     4081 agcaggaatg gatgtttctg gccgggcgag gagcctttcc gctttttgcc ctggtgtggg
     4141 ggctgaatct gtcccgtcat gcgcatatcc ggcaaccggc cattaaccgg ctgtggggat
     4201 ggggaattat tgcccagttc gcatattatc tggcaggctt tccctggtat gaggggaata
     4261 tcctgtttgc ctttgcagtg gcggcacagg tgctgacgtg gtgtgaaacg cgcagtgggt
     4321 ggcgtacagc agcggccatt cttctgatgg ccctgttggg ggcctttgtc cggcaccagt
     4381 tacggcattg ccgggctgct gatgctggca gtcagccacc gactgtaccg ggcggaagac
     4441 agagcggaac gtctggcgct ggtcgcctgc ctgctggctg ttattccggc gcttaacctt
     4501 gccaccagtg atgcagcggc ggtggccggt cttgtgatga cggtgctgac cgttggtctg
     4561 gtgtcgtgtg caggaaaatc attaccccgt ttctggcccg gggatttttt cccggtattc
     4621 tacgcctgtc atctggctgt gctgggcgtt ctggcgctgt gacgggtgtg gtatctttga
     4681 ccgtaacagg atgattcgtc agaggcagaa cacagcatga cagagcagaa gcgaccggta
     4741 ctgacactga agcggaaaac ggaaggggag acgcctgtcc gcagccggaa aaccatcatc
     4801 aatgtcacca cgccaccaaa gtggaaggtg aaaaagcaga agctggccga gaaagcagcc
     4861 cgggaagcag agctggcggc aaaaaaagcg caggccagac aggcactgtc catttatctg
     4921 aacctgccca cgctggatga ggccgtgaat accctgaaac cctggtggcc gggattattt
     4981 gacggtgaca ccccccggct tctggcctgc ggtatccggg acgtgttact ggaagacgtg
     5041 gcgcagcgga atatcccgct ctcgcataaa aaactgcgca gggcgctgaa ggccatcacc
     5101 cgttcagaaa gctatctgtg tgccatgaaa gccggtgcct gccggtatga cacggaaggg
     5161 tatgtgacgg agcatatttc tcaggaggaa gaagcgtatg cggcagcgcg tctggataaa
     5221 atccgccgcc agaaccggat aaaggcagaa cttcaggccg tgcttgatga gaaataaaaa
     5281 aaagcctcca tcagagagga ggcagggaaa taaggcttaa aaggaaataa cttccgcttc
     5341 cgaaaggaag ccagtccact gaattattta tcacaataat actgacaact aaagggggac
     5401 aagaaaatga atggattcag aaacagttca cggaacggcc aggtctggcg ttaccagcgt
     5461 gccggcgggc gggccgttat tctggaagtc agtggacgct ggatggaagc ggcagaagca
     5521 tggcgacggg ctgcctgtgt tgcgcccagg acagactggc agcagtttgc ccgaaaaagg
     5581 gctgaacact gccaccggcg ctgccgggga agggtgtaac gaaaaaagca gccttcatta
     5641 ctccaggagt cagaaggctg aaaggagttt atgagatttg ctttttttaa tatccggtcg
     5701 ggcagtatga ccggacagtt ctgagtagca cagagttttt tccgtattaa gtcgtgattt
     5761 tatattttgt gattaatttc acaaaataag gtgttattca gtgtgtgctg caatattcag
     5821 gatgcctgaa atacagatgc tatttatctg atatggagaa taacatgagg aaatagtaag
     5881 gcagtatgac tttccgtgag acagatgctg gccaacgtgg gatatttaac gcgggttaa
//
LOCUS       DAJVRF010000192         5936 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJVRF010000192 DAJVRF010000000
VERSION     DAJVRF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.192"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            1..487
                     /locus_tag="OQN13_003694"
                     /pseudo
     CDS             1..487
                     /locus_tag="OQN13_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(529..981)
                     /locus_tag="OQN13_003695"
                     /pseudo
     CDS             complement(529..981)
                     /locus_tag="OQN13_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1045..1191
                     /locus_tag="OQN13_003696"
                     /pseudo
     CDS             1045..1191
                     /locus_tag="OQN13_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1191..2348)
                     /gene="intS"
                     /locus_tag="OQN13_003697"
     CDS             complement(1191..2348)
                     /gene="intS"
                     /locus_tag="OQN13_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCS1616940.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2510..2584)
                     /locus_tag="OQN13_003698"
     tRNA            complement(2510..2584)
                     /locus_tag="OQN13_003698"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2549..2551),aa:Arg,seq:cct)
     gene            complement(2660..3592)
                     /locus_tag="OQN13_003699"
     CDS             complement(2660..3592)
                     /locus_tag="OQN13_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCS1616941.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3886..4641
                     /gene="mlaA"
                     /locus_tag="OQN13_003700"
     CDS             3886..4641
                     /gene="mlaA"
                     /locus_tag="OQN13_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCS1616942.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(4823..5158)
                     /locus_tag="OQN13_003701"
     CDS             complement(4823..5158)
                     /locus_tag="OQN13_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616943.1"
                     /translation="MAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDD
                     AKFRRDNVKEIISNLFANKVDVDHPENKYKDFKDLEDNVEKRLQNRQAKYQNEINQLS
                     APGVNFDDI"
     gene            complement(5116..5508)
                     /locus_tag="OQN13_003702"
     CDS             complement(5116..5508)
                     /locus_tag="OQN13_003702"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1616944.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLF
                     TWYGKDKKGNSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARN
                     ASAMLQRGLKAVKLSWQNHLQQVPIIRL"
     gene            complement(5515..5891)
                     /locus_tag="OQN13_003703"
                     /pseudo
     CDS             complement(5515..5891)
                     /locus_tag="OQN13_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937824.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1476 a         1336 c         1310 g         1814 t
ORIGIN      
        1 acttcagttt aattaaagga ttaattagtt gggctaatca ctgtgctgaa tacatagaaa
       61 tttttgatga gtctattgct tttacatgtc tctctgcatt ttcttctgaa aaacaatttg
      121 gagtatttct gtttggatgt ttgaaaagca cagggctaaa gttaaaacga ttattcatac
      181 ggatttatct gcaccatggc gtcttaagga tatatcatca agattatatc tcagcgaaag
      241 tttactaaag atgaaattga aagaagaggg ggtatcattc agtaagatca tacttgatga
      301 gaggatgcaa atggctgaat atttactcag cactcgttgt tatcctatta gtaaagtagc
      361 taaggtctgt ggttatgcca gtgtctcata ctttacttat gtatttagac gttattttgg
      421 tgtttctcca agtcaatact ctcagaggag ttcagaaagt aaaattctta ctcaccaggg
      481 aatctgatca ttgttcttgc ccccttattt ccagacaggg ggtgtatctt aagttaacgt
      541 tacccgctga cgtcgatatt ctcgcggaga gcgataaccc agcgcactgt gcggatggtt
      601 ttcattgtaa tgttcgatcg ccactgcaag attatgcaat gccgttctta cattcggttt
      661 cggcatgaac gcgatgtagt cttccttcat cgttttcacg aacctttctg ccattccatt
      721 actctgagga ctgctgattg ccgttgtgca gggctccaga ttcaactctc tggcgaactg
      781 ccgtgtttca tgcgctctat acgctgaacc gttatctgtc agccactgga tggactgttc
      841 cggtacttta tcgccaaagc gtttttctat cgctcctaac atgacatcct gcactgtcgc
      901 tttatcgtaa cctcccgtgc ttgcggccca gtctatggct tcacgatcgc aacagtccag
      961 ggcgaacgta acacgcagtt ttgaatcgcc acgggtttaa cagacacctc agagtcattt
     1021 aagatggctt aaagagaggt gcccatgagc ggtaagcgtt atcccgaaga gtttaaaact
     1081 gaagcagtca aacaggttgt tgatcgcggt tattctgttg ccagcgttgc aacacgtctc
     1141 gatatcacta cccacagcct ttatgcctgg ataaagaagt acggtccgga ttactccact
     1201 ttttcatcaa gccaatccgc ccaccactgc atcatttctc tgcgcttatc gagatactga
     1261 gcatggttgt aaataccgcg cacagatccg ccgttggcat gtgccagctg cacttcaata
     1321 gcgtcagcag gccattcgtg ctcgttcata atcgtgctga attcatgcct gaatccgtga
     1381 ccgctttcca gaccttcata gccgatttgt ttgatcacaa gcaataccgc gttctcgcag
     1441 attggcttct tcttatcgtt gcgcccggca aaaacaaact ctgagactgg tttggtgatt
     1501 gagcttagcg tagtgagaag ttcaaccacc tggtctgaca tcgggaccac atgaattttg
     1561 cgtcccttca tcacactggc gtcgatggtg ataatcctgt tttcaaaatc gacgttcttc
     1621 catagcatgg aacgaagctc ttttgttctt agggctgtgt agcgtaaaac tttggtcgca
     1681 atgagcgata cgatacttcc tgaaaatgtt gccagtgctt tgttgaatgc aggcatctgg
     1741 tctgctggaa ggaacgggaa gttcttcttg cggtatcctt tcatggcgtc tgcaaggtca
     1801 ggtgccgggt tatatttagc ccttccggtg acaatagcgt aacgaaaaac ctcgccgcat
     1861 cttctgcggg ctttgttggc tcgctccatt gcaccgcgat cttcaaatct gcggattact
     1921 tccagcagtt gcatcggctc aatatcctga atctcaagac cgccgatgat gggtaaaatg
     1981 tcgtcatcaa acattttggc aagttcagtt gcatagccta ctgaccagac ttgcttcttg
     2041 tgctcgtacc attccttgta aatcgcacta aatgaattgt tgttagacga agcctttttc
     2101 gcctttaccg gaacgatgcc aaccgagatg tctttcctcg cagtccatgc cttatccctt
     2161 gcttcctgca aagtcataag cggatatttt ccgacggtca ggattttctc cttaccgtca
     2221 atcttgtagc gaagctgcca tacctttttc ccggatacag gaacataaag gtacaggcca
     2281 ttaccatcga gaaggcggta tggtttttct ttcggctttg ctgcttcaat ctgcttaacg
     2341 gtgagcatgg gtaaaaatcc ggtgggtaaa attattttat ccacttttta cccgtcatgg
     2401 agtgcggctg tcaacgatct gacgcgaacc atgacgaacc gtgaatatac ggaaggcttg
     2461 atattcaggg gattttgcgg actggtacgg atgggagcga actgataaat ggtgtcccct
     2521 gcaggaatcg aacctgcaat tagcccttag gaggggctcg ttatatccat ttaactaaga
     2581 ggacaatgcg gcatgagtat acccgctaat ggagtgcggg gtaagtacgc tgccgctcga
     2641 ttgcttaaac cctcgccatt tatgccgggt ttttatcatt tttcttaatg ttttccgcac
     2701 gttctgcttt ttggcgtgct tctgctttac gcttgttgct catgtcgtta cgaatctgtg
     2761 catgactcat taacgcgaag ataaaggtgc cgccgcagat gttccccgct aaagtaggta
     2821 gtgcgaaggg ccagatgaaa tcgctccagt gcagcgtacc gttaaacacc agatagagga
     2881 tttcaacaga accgaccacg atatgggtgg tgtcacctag ggcaataagc caggtcatca
     2941 atataatcac cacaatcttt gccgcacctg ctgcaggaaa catccatacc atagtggcga
     3001 tcagccagcc ggaaatgatc gcgttggcaa acatctcgct gggggtgttc ttcatcacat
     3061 ccatgccgat tttgacaaat gcatcgcgag tttcttcatt gaagataggc atatattcaa
     3121 atgcccacgc agcaatacca gtcccgagaa tattacccag cagcacgact ccccataacc
     3181 gcataagtaa gccaacgttg ctcattgtcg gtttttgcat gacgggtagt accgcagtca
     3241 cggtgttttc ggtaaataat tgctggcggg ccattatgac gataataaaa ccaaaggtat
     3301 aaccgagatt ctccagtaag aagctgcccg gcacaccttc cagttcgaca tgaaatatcc
     3361 cttttgccag taacgaagcg cccatcgaca gacccgccgc aatggctgac cacagtagcg
     3421 ccattgcgtc gcgttccagc tctttttcac catcctggcg gatatgctca tgaattgcca
     3481 tcgcccggga ggggagtcgg tcttcatcta tttctatttt tttgccgcgc tctttttctt
     3541 cgctctcaac ttcaatttcg tcgctgtgtt gatcaatttt gtcgttgtcc atggtctctt
     3601 ctcttgaatt agcacgtata gctaaagcgt agcggctttt ttgctcgcaa ctggcgggag
     3661 ttactctgaa aatgtagaaa aggctgcgtt tgcctttttc tgtttctata gaatcaagta
     3721 gcctacaggg cggcgattac caggctatga tcaaatcagc aaatcagggc gtctggacat
     3781 cagttgacgt gctgttacaa tcgcccacac ctaaacaggc ggatacggta tcgttccgtc
     3841 atggatggca aactgcataa gccataaaaa aacagggaga catttatgaa gcttcgcctg
     3901 tcggcgcttg ctctgggaac tacgcttctg gtggggtgtg cgagttccgg tacagatcag
     3961 caagggcgtt ctgacccgtt agaagggttc aaccgcacca tgtacaactt caacttcaac
     4021 gtattagacc cgtatattgt tcgaccggtc gctgtcgcct ggcgtgatta tgttccgcaa
     4081 ccggcgcgta atggtttgag caactttact ggcaaccttg aagaacctgc ggtgatggtt
     4141 aactacttct tgcagggcga cccttatcag gggatggtcc actttacccg ctttttcctg
     4201 aacaccattt tggggatggg cggctttatt gatgttgcag ggatggcgaa cccgaaactg
     4261 caacggactg aacctcaccg cttcggtagt acgcttggtc attatggcgt gggttatggg
     4321 ccttacgttc agttaccgtt ctacggtagc ttcacgctgc gtgatgacgg tggtgatatg
     4381 gcggatggtc tttacccggt tctttcctgg ctgacctggc cgatgtctgt gggtaaatgg
     4441 acgcttgaag ggatcgaaac ccgcgctcag ctgctggatt ccgatggtct gctgcgtcag
     4501 tcgtccgatc cttatattat ggtgcgcgaa gcgtacttcc agcgtcatga tttcatcgct
     4561 aatggcggcg aactcaaacc gcaggaaaat ccgaacgcac aagcgattca ggatgattta
     4621 aaagatattg attctgaata agaaacaaat aaaaaaggtg agtcgcaaaa ctcacctttt
     4681 ttgttgttat ccctcaatta ccacaagtaa aaggagataa accgacaatg acccggacgt
     4741 aatgatgtaa aaatactgat gttcgtcact gactatttcc gatattcagg cgttgaaaaa
     4801 tcaaaaaact ggaaaaataa ttttaaatat catcaaaatt aacacctggc gcagatagtt
     4861 gattaatttc attttgatat ttcgcctggc gattttgaag tcttttttca acgttatctt
     4921 cgagatcctt aaagtctttg tatttatttt caggatggtc aacgtcaact ttgttagcga
     4981 ataaattaga aattatttct tttacattgt cgcgtctaaa ttttgcatcg tccacttgaa
     5041 aattatatct tattgctcga cctaaacgtt taaaaacttc gttaggttct ctcagtcttt
     5101 ccccgttgag ctcgtttaca acctgatgat cggaacctgt tgcaaatgat tctgccatga
     5161 tagctttact gctttcaaac cacgctgaag cattgctgaa gcattcctgg ctttttcact
     5221 acaatattgg gtgagactat caccattaat acgaattcct ctttgaatgt ggtagtcgtc
     5281 ctgatcgtag taaccgagag ataagtaatc atttccgttt ttatttttaa ttacaatagc
     5341 gagagagttg ccttttttat ctttaccgta ccaggtaaat aagtccgttg gttcgctatt
     5401 cccgctctca tcaaattctg ttaccgtatg tctgagtttg tgtgatatct tatcgaagat
     5461 aaaaccagga tctccatcat atatttcttt taatgcgctg aagatcattt ccttgttatt
     5521 gatatcatga gtcttctcaa ctatgttgtc attaatgata gtacggacga gttctttgtc
     5581 tttatagttc gcaggtaatt ctattggttc ttggttgtcg ataccaatag taatatggtc
     5641 tgtatcattg gagccaatat caatataagt aactgaggaa ttattaatat taaatcttaa
     5701 ggatcgtcgt ttactcgtat ggcgtttttt ataagctcaa gtagatgttt aatacaggtg
     5761 cttattcctt ccttaccatt aaaaaggttt ttgagctctt gtactaatcc attaacttca
     5821 tcattgttat tttcattaat ggattctggg taagttgcgc tggtattgtt gattgaaata
     5881 gatggtagca tgattttatt ccattttgat tgatttgtta caaatatata ttacct
//
LOCUS       DAJVRF010000193         5922 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJVRF010000193 DAJVRF010000000
VERSION     DAJVRF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.193"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="OQN13_003704"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="OQN13_003704"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCS1616945.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="OQN13_003705"
     CDS             complement(1672..2730)
                     /locus_tag="OQN13_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1616946.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="OQN13_003706"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="OQN13_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="OQN13_003707"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="OQN13_003707"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCS1616947.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..>5922)
                     /gene="emrY"
                     /locus_tag="OQN13_003708"
     CDS             complement(4570..>5922)
                     /gene="emrY"
                     /locus_tag="OQN13_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCS1616948.1"
                     /translation="GVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSLSTNL
                     DVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPILGGY
                     ICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVGGLQI
                     MLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTIGIVS
                     ITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNKIDMR
                     LLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFSGLPD
                     NKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSSSQIM
                     DKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTAKGVG
                     "
BASE COUNT         1646 a         1431 c         1399 g         1446 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgtcata
      121 gttaacgtta acatttgtga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaacaatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgccacctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagcg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat tgtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg gaccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tgttttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcggac aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaggagaac ctgataagcg acaaaggctg cacgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgggcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aggcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataacgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccgctgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctag
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccagc tatgcaaaaa cggctggaaa aagaatatca
     3421 gcaaccgatc agcgggcaac tgttactgaa aaaagatagc catttgccca tttccggctc
     3481 cataaaagca cgcggcggga tttatgaagt cctggcacac gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc ggcactggcg ggtatggccg gacctcagcg
     4321 cgtatgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aactgcgtaa
     4381 taccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccataata
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctgaaaa aactgcggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaacta aatgtcacta acaaccgcat
     5101 gtctattttg ttgccataac gtcctatcaa aggtgaaatt aatagtggca tgatgccgat
     5161 gggcgcataa gcaagtccgg cccatatcgc attatacccc atcgtttcct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg catgtgatac tcacaatacc
     5281 aatggtgaag ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ctcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcctggtag attcattttg accggtgaag tctcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc acaaatataa ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 cggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaaccc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataggctac acattaatga
     5821 agacagcgaa aaaaaggtta ctgaaagtaa aaataatctt aattcgccta ttctttgtgc
     5881 caacctgcca gtaacaggga tcgcaatggc atttgctaca cc
//
LOCUS       DAJVRF010000194         5838 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJVRF010000194 DAJVRF010000000
VERSION     DAJVRF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.194"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(21..1958)
                     /locus_tag="OQN13_003709"
     CDS             complement(21..1958)
                     /locus_tag="OQN13_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCS1616949.1"
                     /translation="MNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSRSGI
                     LALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPPFYD
                     TLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGDLLI
                     HEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKSNHH
                     GALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFRLAI
                     IQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNENDP
                     GIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYPLFA
                     ALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDYPTS
                     VLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVPATI
                     LAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAEVLP
                     SVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAHSAD
                     MTFRVKPESLILTIFFLGLIP"
     gene            2369..3076
                     /locus_tag="OQN13_003710"
     CDS             2369..3076
                     /locus_tag="OQN13_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCS1616950.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3182..3257
                     /locus_tag="OQN13_003711"
     tRNA            3182..3257
                     /locus_tag="OQN13_003711"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3215..3217,aa:Phe,seq:gaa)
     gene            3455..4426
                     /locus_tag="OQN13_003712"
                     /pseudo
     CDS             3455..4426
                     /locus_tag="OQN13_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            4564..5583
                     /locus_tag="OQN13_003713"
     CDS             4564..5583
                     /locus_tag="OQN13_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HCS1616951.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
BASE COUNT         1563 a         1480 c         1453 g         1342 t
ORIGIN      
        1 gatttactgc tacgtcgccc tcagggaatt aaacctaaaa agaaaatcgt caaaattaga
       61 gattccggtt tgacacggaa agtcatatca gcgctatgcg catcctgggg gttcatcacc
      121 actgacggga aactctgctg gcggaacatc gctttttgta ggtctttgac gtcgaaactg
      181 acatacagat cgtgcatctc ctgacacaac tggcgcaggg tgtagtcgcg atagcgcacc
      241 ggatatttgt tataaacgct cggcaacacc tcagccagcg gcgagtcatc ctcaatatgc
      301 tgttcaaatt gcgccagcat cgccaccagt tgtgccagct tctcgtggct ttccgccgga
      361 gttaataaga acagaatgga gttgagatcg cacttctccg gcacaatgcc gttctcacgc
      421 agatagtgcg ccagaatcgt cgccggaacg ccaaagtcgc tatattcgcc ggtttcggca
      481 ttgatacctg gtgtagtgag taacagcttg cacggatcaa caaaatactg atccgcggca
      541 tatccttcaa agccgtgcca cttcgccccc ggctcaaaac tgaaaaaacg gcggtcgctg
      601 gctaacacag atgtcggata atcctgccac aatttgccat caacaacggg cgggataaac
      661 gggcggaaca gcttacagcg cgcaagaata gccttgcgcg actcaatacc caactcaacg
      721 cactcagccc acagccgacg cccactctcc ccttcatgaa ttttggcgtt aacatccagt
      781 gcagcaaaca gcggatagaa agggctggta gaagcatgga gcataaaggc gttattcaac
      841 cgcttatgcg ggcaaaaacg cgcctgtccg cggatatggt tatctttttt atggatctgc
      901 gacgtctgtg agaatcccgc ctgctgtttg tgcaccgact gagtcacaaa gatccccgga
      961 tcgttttcgt taagttctaa tagcagcggc gagctatccg ccatcatcgg gataaactgt
     1021 tcgtaaccga cccacgcgga atcaaacaga atgtaatcac acagatgccc aacggtatcg
     1081 atcacctgac gggcgttata gacagttccg tcataggttc ccagctgaat aatcgccagg
     1141 cgaaacgggc gcggcagctc ggctttttct ggcgcaacgt cgcgaatttg ctggcgcaga
     1201 tactcttcat taaaacagtg cgcatcaata ccgccaatga aaccaaacgg gttgcgtgaa
     1261 gcttccagat agaccgacgt cgcccccgcc tgaatcagcg cgccgtgatg attcgactta
     1321 tggttgttac ggtcgaagag caccagatcg ccacgcgtta acagcgcatt cgtcaccact
     1381 ttattcgctg ccgatgtgcc gttcagcaca aaataggttt tatcggcatg aaagactttg
     1441 gctgcgaatt tctgcgcatc cttcgccgat ccttcatgaa taagcagatc gcccaatttt
     1501 acgtcagcgt tacacatatc ggcgcgaaag acgttctcac caaagaaatc gtaaaaatgg
     1561 cgtccggcag gatgcttttt aaaaaacgca ccatgttgat gtccagggca agcaaaggtg
     1621 ctgttgccca tctcaacgta ctgcgtcagc gtgtcataaa acggtggcag caaattctct
     1681 tcatactgac aggctgcgga ttccagctcc agccactgct gctcgttgcc gttgattacc
     1741 gccgtaacgc ccgcaggtaa ttcaacagca tgttcggaat acaaaaacac cggtagatga
     1801 aaaccggtgc gcttaagcaa cgcaagaatg ccactgcgac tatccgcagc ggtaatgacg
     1861 actgccgcga catccgtaaa atcagtatct cccaacgcca ccacgcggcg atgagtagaa
     1921 agtcgggata ccagttcact actggcggca atattcattg atttcataag cgcaaaaccc
     1981 gtttcgggga agtaagaata ccggacaaag tgtaaaaccc tgccccatga gatatgggtc
     2041 aaactggtca ccagctccga cgaccgccag acatcagtaa aagcagaaac gctctgattt
     2101 tactgttgtc ctgcagtgaa cgtgcgttaa cttcaccgca tgagcagtaa catagaaagg
     2161 gaaacgtttc gcgcgaaacg gcgataagcg agagaatgta aggagatggc gtgcatcggg
     2221 caaactccgt acagagagga gaaaattcgc gcaatcatgg cacctttcgc taaggcgtgc
     2281 aagccaggac tttgcgacca gacaagccat caacaaggca aaaagttcat aataagaaaa
     2341 tcaaacaata cagctaacag gaactcttgt ggtcatcggc ccttttatca acgctagtgc
     2401 agtcttactg ggtggcgttc tcggcgcact gctcagccaa cgcttaccgg aacgtatccg
     2461 cgtctccatg acatctattt ttggtctggc atcgctgggg attggtattt tactggttgt
     2521 gaaatgcgcc aaccttccag cgatggtttt agctacgcta cttggcgctc taattggcga
     2581 aatttgtttg ctggagaaag gtgtcaatac agcagtcgcc aaagcacaaa atctgtttcg
     2641 tcactcacgt aagaagccag cgcatgaatc ttttattcag aattatgtcg cgattattgt
     2701 cctgttttgc gccagcggca ccgggatctt cggggcgatg aacgaaggga tgaccggcga
     2761 tccgagtatt ttaatcgcca agtcatttct tgatttcttt acggcgatga tcttcgcctg
     2821 ctcgctgggt attgcggtgt cggtaattag tatcccatta ctgatcatcc agctaacgct
     2881 agcgtgggct gccgcgctga tattaccgct gaccacaccg tcgatgatgg cagacttcag
     2941 cgccgtaggc ggtttattgc tgctggcaac cggattacgc atctgtggca ttaaaatgtt
     3001 cccggtggtc aacatgcttc cagcactcct tctggcaatg ccgctttccg ccgcctggac
     3061 cgcctggttt gcctgacaat gcgtgcaata gcggcaaagt gatgatagat tgtgcagtct
     3121 gcagtaaatt gaagaaattt gattgacgag acgaggcgaa tcaggtttaa tgcgccccgt
     3181 tgcccggata gctcagtcgg tagagcaggg gattgaaaat ccccgtgtcc ttggttcgat
     3241 tccgagtccg ggcaccaaat tcatatcaac ggacctccac ggaggtccgt ttttcgtttc
     3301 agaacgccac gatttaagcg ttctgcctac aaatcaattc taccgaactc aaccagattc
     3361 cccccacatc aaccccattg tgtgggtata attgcgggca taccccagtt cgacagaatt
     3421 tgtaccctct ttaacgcatt cagaaggctg aactatggca ctgactgacg caaaaatccg
     3481 ggctgcaaag cccactgaca aggcttataa actcactgac ggggctggca tgttcctgct
     3541 ggtacatcct aatggctccc gttactggcg tctccgttat cgtattctgg gtaaggagaa
     3601 gactctggca cttggtgtgt atccagaagt ttctctctcc gaagctcgta caaaacggga
     3661 tgaggcccga aaactgattt cggagggggt tgacccttgc gaacagaaaa gagctaaaaa
     3721 agtagtccct gatttacagc tctcttttga acatattgcg cgacgctggc atgccagtaa
     3781 taaacaatgg gcacaatcac acagcaataa agtactcaaa agccttgaga ctcacgtttt
     3841 cccctttatc ggcaaccggg atatcacaac actcaatacc ccggatctgc ttatccctgt
     3901 tcgtgctgca gaagctaaac aaatttatga aatcgccagt cgtctgcagc aaagaatatc
     3961 tgccgtaatg cgttatgccg tacagtctgg catcatcaga tataatcctg ctctggatat
     4021 ggctggcgca ttgactacgg taaaacgcca gcatcgcccc gctcttgatc tttctcgcct
     4081 gcctgaactt ttgtcgcgta ttggcagtta caaagggcaa cctgtcaccc agcttgccgt
     4141 tatgctgaat ttactggttt ttattcgttc cagtgaactc agatacgccc gctggtctga
     4201 aattgatatt gacaatgcca tgtggactat tccagccgaa cgcgaacccc tgccaggcgt
     4261 aaaattctca caccggggct ccaagatgcg aacaccacat cttgtgccac tcagcaaaca
     4321 ggctgtagcc atactgacag aactacagac atgggctggt gaaaatggtc tgatctttac
     4381 gggagcacat gacccgcgta aaccaatcag tgaaaatgta atgaacgcat cccacacaat
     4441 taaacacgga gcagtatgat ggttaaaagg gtgaaactgg caggtgtgga gcctcgcaga
     4501 tttccggcat catagtgccc aatacgggat gcataaagct ccctcagact ggagactcca
     4561 caaatgaaat atacaccggt tggcgttgat atcgcaaaac atgtcattca gattcacttc
     4621 atcaatgagc acacaggtga agtggttgat aaacagttgc gtagacagga ttttctgacg
     4681 ttcttcggca accgtgagcc atgcctgatt ggtatggagg cctgtggagg ttctcagcac
     4741 tgggcacggg aactgacaaa acttggtcat aaagtccggt tgttgcaggc ccgcttcgtt
     4801 aaggcattcg tcatgggcaa taagaatgat gtgatggatg cccgggctat ctggatggcg
     4861 gttcagcagc cgggtaaaga aatcgccgta aaaacagaag aacagcagtc ggtactggtt
     4921 ctgcaccgta cccgcatgca actggtgaag ttccggaccg cacaaattaa tgccctgcac
     4981 gggacgttac tggagtttgg tgaaaccatc cacaaaggcc gggcagcgat ggagcgggag
     5041 ttccccgaag cactggaacg gatgaaagag agactgccac cgtatctcat tatggttctg
     5101 gaaaaccagt acaaccgact gaatgagctg gactcactga tagaggatat tgaaaaacag
     5161 cttaccagcg tggcgaggca gaatgaaacc tgtaagcggt tgctggatat tcctggcgtt
     5221 ggaccactta ttgcgacggc agcggtggcc accatggggg aagcatcagc gtttaaatcg
     5281 gggcgagagt tcgccgcata tgttggtctg gttccaaaac aaactggctc cggagggaaa
     5341 gtacgtctgc tggggataag caaacgtggt gacacttatc tcaggacatt atttatccac
     5401 ggtgcaagag cggtggcatt agtagctaaa gagcctggcc cgtggataac cgaactgaaa
     5461 aaacgtcgtc cagccagtgt ggcaatcgtc gccatggcaa acaagctggc acgaacagta
     5521 tgggcgataa ccgcccatga ccgtaagtat gacaggaacc acgtcagtat cagaccatat
     5581 taatcgctga taccattaaa caatgaactc ttaacaaaag ggtgaatgct gaaaggttgc
     5641 tatggcggcc agagtgatga caaagacagg taagaccgtg actcactaaa cctgaacagt
     5701 attttgggct tgaagtccgc cgtgaaaata aggggtgagt cggcgaatta cataggggct
     5761 cgcagcgtta cggctgcaat aaagccggat ataaagctgc aacctacccg tcatgtcaaa
     5821 acaatggatg ccttgcaa
//
LOCUS       DAJVRF010000195         5836 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJVRF010000195 DAJVRF010000000
VERSION     DAJVRF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.195"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..242)
                     /locus_tag="OQN13_003714"
     CDS             complement(<1..242)
                     /locus_tag="OQN13_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1616952.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(604..3387)
                     /locus_tag="OQN13_003715"
     CDS             complement(604..3387)
                     /locus_tag="OQN13_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS1616953.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(3444..5816)
                     /locus_tag="OQN13_003716"
     CDS             complement(3444..5816)
                     /locus_tag="OQN13_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCS1616954.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
BASE COUNT         1488 a         1320 c         1311 g         1717 t
ORIGIN      
        1 ttaatggata aatccatcaa tttgtggaca ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcgt cattgataat ctggaatgcg
      121 acatcgtcgc gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      181 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      241 attttaaact ccttaaaatg attggatagc attaaaaaag taggatttat cgataaagta
      301 agcaagtgca aaggactcgt gtttaaataa caaaatccta atgttattta tcgtgagata
      361 ttacgagaat aatatttttt cattgaaaaa caatataata tgaaattctt gggtggtggt
      421 aaggtgtttt atgctgttat ttttatgcgc attctgtgcc tcctgaattt tcacgtaaaa
      481 atcagacctt aaaatatcac tattagtacc tggttattat tttgaacgca tttataaaat
      541 tattacataa aaatggcgaa tattgctaaa atccccgcca acgatgtgtt gacggggctg
      601 ttattatttt ggcaataata ctccagtata agtatttacc ggatgagaaa gatattgttt
      661 aacggcagtg ttaacattct ctaccgtcat ttgtttcaac aattgctcct gctcagtcca
      721 tgctgcagga tcgtcatatt gaataagact atttacaata gtgttcgcta attgttgaac
      781 gctacgctgt tggatatcga ggctgcgctg aacgttttgc tggtattcat tcagttcttg
      841 ctcactgatc cctttagcca gacgcttaac catcacttca ttcgctaacg ttaacagttc
      901 atcatgtcgt tctggttgac aagtaaaagc cagcaaatga ctgatatctt tggcctgagg
      961 atcaaccgag aggcgagaag aaacgctgta tgctccagat gcctgttcac gaatattaac
     1021 gcgtagatct tttgccagtg cgacgttaaa agcatcgagc gccatacgcg tcgccagatt
     1081 aacaggtgtc cgggaatcat aacgcttcca ctgtgaaacc tgtgccacag gttcattttg
     1141 ttcttttaca gtaaccgatg cgttgtccgt cgcgcgagtt aatggtttac ctgcggctaa
     1201 tggcgaatca gagtgtttga ttgatcctaa gtaacgcgta attaacgcca cgagtttgtc
     1261 ttctgagaca ttaccgacaa tgacaaacgt gatatccgct ggagatgaaa acaactggcg
     1321 atcggcagcc agcgcatctg cggcagtaaa ctgtacaatc tgattttctt gcagtaattt
     1381 cgtgcggtca tcagcatagc gcgtctcata catctgctgg gcgaatttct ccgctggacg
     1441 ctggtcgagc gttttcaacg cctgaatttg agcattttgt agcgatgccc aaatattgtc
     1501 gttaatcgtg ctgtgggtga ttcgctggtt aatcaactga aaaccaggtt cagggttatt
     1561 agtccgcgcg ctaacggaga gcaacgtatt catgccactg actttactgc tcatggttac
     1621 cgaattttcc gcactccagc gtttcaggct ggaagaggag agctcaccaa cgccgcttcc
     1681 gcttactgct ttatttgcca gcgcgataag tgatttttgc tgcgcaggga aacttaaatc
     1741 gcctttatta gatacggcag taatttgtag cttttgctct tcacccgcgg attttgccag
     1801 aataaccctg gcaccattgg aaagtgttaa tgatgtcaga ttctcagcca gcgtttcttt
     1861 gctgctaatt tccgcctgtg ggtcagcgtc tactgttaac gataaatttc tgcctgggaa
     1921 gatgtaagcc gccagctttt tgttggcgta ctctttttcc agcgccagga tagccgcaag
     1981 agacaatgct tttttggcgg caagctcatt gtttaccatt tgctcccaaa atgcgtcctg
     2041 gttctttctt aactgctgcc atttttccgc cagactttgc acggtaattt gctgccacag
     2101 acgtttcgaa agttgatatg tctcttccgg cgacaagaac ggcgtattat ttaatgagct
     2161 ggatgccagg cgactggtca gcatacgtaa atcacgttca gcttgctgat caaccgcatt
     2221 tttcagccag gtgaggcggg tagatttgac atcatcgagt tcttcagcag aaaaaccatg
     2281 ctgatcaatg gttgccaact ctgccattaa tgcatttgca gcatcctgca tattatcgtc
     2341 tcgtgcattt acacggaaaa acagcgactg ataatcgggt gcaattttaa cgctgcgcgc
     2401 agtgccgcca gaaatagtct tcaactcgcc cgactgtatg cgttcctgca gacgttgatt
     2461 gaacagctga actaacatgc tccattcagc ttgttcgaca aagctttgct catcgttcac
     2521 ttgtaccatt ggcaggcgat aatagagtgc gatgccgttc actcggtttt ctttatcatt
     2581 aatgatatta aagcgcaggt ggttttcggc ttttgtcggc cagacgcgat tttcagctgc
     2641 tttgttagcc ggaagcttac ttaaattatc ctttatcagc gccagcgctt ctttactgtc
     2701 gatatcgccg accacgataa aggtcatatt atttggttga taccagcgtt gataaaattg
     2761 gcgcaattgt gccggtgtga ccgtggcgac agtatccatc aggccgatag gttcacggtc
     2821 taaattacgg gtatttgcca gcaggaaagg gcggcgcgcc tgagaggtgc gccatttcgc
     2881 atcctgatgg gcacgccatt cctcagtaat tacgccgcgt tcagcgtcta cttcgagtat
     2941 ttcaaaggtt gcggcattac tccattcact gaagattgcc atcacttgtt gcagattttg
     3001 tttctgcgta gtcggcagac tcacctgata cagcgtttcg tcatagctgg tataggcatt
     3061 aacatcgcga ccgaaacgca ggcccattga ctcaaatgtt tcgatgactt tattacccgg
     3121 ccatgttttt gtgccgttaa acatcatatg ttctacaaaa tgagccacgc cgcgctcatt
     3181 gtcttcttcc tgcaatgaac cggtatgaat ttgcagccat aaatttacct gatcctttgg
     3241 ttgagcatgc ggataaatca tatatcgcaa gccattgtcc agttgcccgg taattaactt
     3301 ttcatcctgc ggtaaggcgg cggcaatcag ccgcccgggg agcaacagag ttgccactaa
     3361 cgtcagtaag aaacagaggt ttctcataat tatctccatg cgaaaaccgg gcgaatttac
     3421 ccggttaagt aaaatccgaa ctattaaaat ttcatgctga catcaagcca gaaagtacgg
     3481 ccactggcgt aggtcgccac accggtgttc gttgtatcaa cagcggtttt gctatccagt
     3541 acattgagaa tatcggcact gatcgtcagg ttttgttgtt tcagaaattg tggcgtccag
     3601 gacaagcggg tgtcccaggt caggctgcta tccagttttt cgtcaacata ctgcttgtaa
     3661 tctgaatatt cgctgatgta ttgcgcattt gtcttaccaa ggataatgcg agctttacgc
     3721 gcttcttgcc aggccagcgt attcgcccac actaacccgc tcggttgatg cgtgaaatcc
     3781 atgtttaagg agatctttaa tgggttatta aaatctgcca ctggaacact atcgtaagag
     3841 accagattac cgttataaac cacctggtta tcaccggtat tgctctcctc ataaccattg
     3901 tttaacgaca agttgccctt gctcttgata tagctaaaga caatttgtgg gttaatatct
     3961 acttggcgga tatgcagggg ttcggccagt tcaaaactga ggctgaacga atgcgttttg
     4021 gttttgccat cgttgttata ttcagtaatg gtggttttag tcgcgctgtc ggtacgactg
     4081 cttttgctga tttgatcatg cgcttcacgg taaacatagt ttgcacgcgc aataacgttc
     4141 ttaccgattt tctgctgcaa tcccattgcc agttcatcgt tataaggcgt tttcaaatcc
     4201 tgataacgcg tcagagtttt attacctgat accgattccg tccagctatt gcggatatca
     4261 cgtaatccca tatcaagaat attcccgcca tagtaacggt tataaccggc ggtaatcatt
     4321 gaggtttgat cagcaaaaat atcccattcc gtcataaagc gcggggagat attgtggttt
     4381 gacagatagt tgtcatagtc ataacgcaca ccgggcatta acgacacatt ccgccagcta
     4441 atgtgctccg ccatatacag cgtgtagttg tcaattccca ggctgccttt acctttatgg
     4501 taaatggtat ggttagtttt ctttccggca gcattaatca cataggattc agactggtta
     4561 tggcgttcag tccatgcatc ggaatagatg tattccgcgc cgaagtaggg ttgatgcgaa
     4621 acatcaccca cggcgaattt ttgccagtcc aggcgtgttt tgaaggtgta attatctaca
     4681 gcctgggaaa tgtgtcctaa tccgccacag gtacaacgcc ctgtaatatc accatatgta
     4741 catgaaagtt cggtgtacca gatgtcatga tcgtgacggg tataatcact aatatgatcc
     4801 caaccaacgg tggtacgtag tttggcccat gcgagttgcg tatccatatc ccatgccaga
     4861 ccataggatt tattacccat ttcgcgatca gactgcggga aggtgctggt attataatca
     4921 cggctggagc cggtatattt taaggttaaa tcgtgggtaa agcggtcgct ggcaaaccag
     4981 gtaaatttgc tcaatgcagt atcgataacg tttttatact gtgcccgacc ggtgacaatg
     5041 ccgtcattcg aaacataatc cgcgcgggtg atatcagact ggcggcgcga taaaccggcg
     5101 gtaacgccga agttatcagc gagttcctga ttaaacgaca aggtataaaa gttcttttta
     5161 aaatctgggg agtaataagt acttcctgaa gagccttgat taaatgcgct cttgttattc
     5221 tcatcgatat gcgatgttaa ccagtccgaa cgcgtagtgc gataacccaa tttcaccttg
     5281 ctatcatcag cgttgaagcg tttgatcttt gcatcaatta ccccgccatt gaagcgacca
     5341 aattcaaccg gcacaaaact gtcataaagc gtcacattgt ccagtaagct gacatcaaga
     5401 taatacccct gtgacatccc gctaatattg gttgcactac tggcatcgga ctcattcgct
     5461 gggttcaggt tattagttgc actaataccg tcaatcaaat aggcattctg gtagggcgac
     5521 gcaccgtgaa tagagatttt ctcagggcga atatctccct ggttcaacga ggtactttgc
     5581 gttgaatcca tgcgtaccgc agggttggtt ctcagcagat cgctgatatt gccattcccg
     5641 gtcggtaaac gctcgatgct ttcactggtg taatgcgtgt tgccgtttac gggtacaggg
     5701 acaggagcat agaccgtcat ctcttcaaaa aaatgcatgt acatgttttt gtcatcgacg
     5761 gcctgcgcca catcagcgcc acataaaatg acgccaggaa taagaactcg cttcatttat
     5821 ttatatctcc gtacag
//
LOCUS       DAJVRF010000196         5715 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJVRF010000196 DAJVRF010000000
VERSION     DAJVRF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.196"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..619
                     /locus_tag="OQN13_003717"
     CDS             <1..619
                     /locus_tag="OQN13_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCS1616955.1"
                     /translation="QKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIETVGVNQ
                     IETVGSNQIIKVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSL
                     RVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLN
                     GTKIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
     gene            687..4229
                     /locus_tag="OQN13_003718"
                     /pseudo
     CDS             687..4229
                     /locus_tag="OQN13_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            5210..5715
                     /locus_tag="OQN13_003719"
                     /pseudo
     CDS             5210..5715
                     /locus_tag="OQN13_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         1433 a         1377 c         1772 g         1133 t
ORIGIN      
        1 gcagaagaac atgaacaccg aggtgctgaa taaccgcacc acggatgtga taaacaacca
       61 tgctgaaacc attggcaaca atcagatgat tgcggttacc aacaatcaga tagagacggt
      121 gggcgttaac cagatagaga cggtgggcag taaccagata attaaagtgg gttctgttca
      181 ggtggaaacg attggacttg ttcgtgcgct gacggtgggc gtggcgtatc agacgacggt
      241 aggtggcatt atgaacacct cggtggcact gatgcagtcc tcgcagatcg gtttgcataa
      301 atcgttgagg gtcgggctgg gttatgacgt caaagtcgga aataacgtta ccttcaccgt
      361 tggtaaaacg aaaaaggatg ataccgggca gaccgcgatt tactccgccg gtgagcatct
      421 ggagctctgc tgtggtaagg caaggctggt gctgacgaag gacggacaaa tttttctcaa
      481 cggcacaaaa attcatttgc agggtaagga gcaggttaat ggtgactcac tgttgattaa
      541 ctggaactgt gcagcctcga aatccccacc gaagacccct gatgaaaagc aggatacgcc
      601 ggatatgaga gagtactgat cccgtacctg ttcggtattc agtaacaaac cagcattaaa
      661 aataaacaga agaaggaatt tgaatcatga gcggaaaacc agcggcgcgt cagggagata
      721 tgactcagta tggcggtccc attgtccagg gttcggcagg tgtaagaatt ggcgcgccca
      781 ccggcgtggc gtgctcggtg tgtccgggcg ggatgacttc gggcaacccg gtaaatccgc
      841 tgctgggggc gaaggtgctg cccggcgaga cggaccttgc gctgcccggc ccgctgccgt
      901 tcattctctc ccgcacctac agcagctacc ggacgaagac gcctgcaccg gtgggcgttt
      961 tcggccccgg ctggaaagcg ccttctgata tccgcttaca gctacgtgat gacggactga
     1021 tactcaacga caacggcggg cggagcattc actttgagcc gctgctgccg ggggaggcgg
     1081 tgtacagccg cagtgagtca atgtggctgg tgcgcggtgg taaggcagca cagccggacg
     1141 gccatacgct ggcgcggctg tggggggcgc tgcgccggat atccggttaa gcccgcatct
     1201 ttacctggcg accaacagcg cacaggggcc gtggtggata ctgggggggg ctgagcgggt
     1261 gccgggtgct gaggacgtac tgccagcgcc gctgccgccg taccgggtgc ttaccgggat
     1321 ggcggacgct gacgtaccgg cgtgaggccg ccggtgacct ggccggggaa atcaccgacg
     1381 tgacggacgg tgccgggcgg gagttccgtc tggtgctgac cacgcaggcg cagcgtgcgg
     1441 aagaggcccg cacctcttcg ctatcttctt ctgacagttc ccgccctctc tcagcctcag
     1501 cgttccccga cacactgccc ggtaccgaat acggccccga caggggtatc cgcctttcgg
     1561 cggtgtggtt gatgcacgac ccggcatacc cggagagcct gcccgctgcg ccactggtgc
     1621 ggtacacgta tacggaagcc ggtgaactgc tggcggtata tgaccgcagc aatacgcagg
     1681 tgcgcgcttt cacgtatgac gcgcagcgcc cgggccggat ggtggcgcac cgttacgcgg
     1741 gaaggccgga gatgcgctac cgctacgacg atacggggcg ggtggtggag caactgaacc
     1801 cggcagggtt aagctaccgc tatctttatg agcaggaccg catcaccgtc accgacagcc
     1861 tgaaccggcg tgaggtgctg catacagaag gcggggccgg gctgaaacgg gtggtgaaaa
     1921 aagaactggc ggacggcagc gtcacgcgca gcgggtatga cgcggcagga aggctcacgg
     1981 cgcagacgga cgcggcggga cggcggacag agtacggtct gaatgtggtg tccggcgata
     2041 tcacggacat caccacaccg gacgggcggg agacgaaatt ttactataac gacgggaacc
     2101 agctgacggc ggtggtgtac ccggacgggc tggagagccg ccgggaatat gatgaaccgg
     2161 gcaggctggt atcggagaca tcgcgcagcg gggagacagt acgctaccgc tacgatgacg
     2221 cgcacagtga gttaccggcg acgacaacgg atgcgacggg cagcacccgg cagatgacgt
     2281 ggagccgcta cgggcagttg ctggcgttca ctgactgctc gggctaccag acccgctatg
     2341 aatacgaccg cttcggccag atgacggcgg ttcaccgcga ggaaggtatc agcctttacc
     2401 gccactatga caaccgtggc cggttaacct cggtgaaaga cgcacagggc cgtgaaacgc
     2461 agtatgagta caacgccgca ggcgacctga ctgccgttat caccccggac ggcaaccgga
     2521 gcgagacaca gtacgatgcg tggggaaagg cggtcagcac cacgcagggc gggctgacgc
     2581 gcagtatgga gtacgatgct gccggacgtg tcatcagtct gactaacgag aacggcagtc
     2641 acagcgactt cagctacgat gcgctggacc ggctggtaca gcagggcggc tttgacgggc
     2701 ggacgcaacg ttatcattat gacctgaccg gaaaactcac gcagagtgag gatgaggggc
     2761 ttgtcaccct ctggcactac gatgcgtcgg accgcatcac gcaccggacg gtgaacggcg
     2821 acccggcaga gcagtggcag tatgacggcc acggctggct gagagaaatc agccacctga
     2881 gcgaaggcca ccgtgtcgct gtccactacg gctacgacga taagggccgc ctgacgggcg
     2941 aacggcagac ggtggagaac ccggagacgg gggaactgct gtggcagcat gagacgaaac
     3001 acgcctacaa cgaacagggg ctggcaaacc gcgtcacgcc ggacagcctg ccgccggtgg
     3061 agtggctgac ctacggcagc ggttacctgg cgggcatgaa gctgggcggg acgccgctgg
     3121 tcgagtatac gcgggacagg ctgcaccgtg agacggtgcg caccctcgca ccggacctgg
     3181 acatccgcat cccgtatgcc accgacccgg cgggaaaccg cctgccggac ccggagctac
     3241 acccggacag cacgctcacg gcgtggccgg ataaccgcat cgcagaggat gcgcactatg
     3301 tttaccacta tgatgaatac ggcagactga cggagaagac ggaccgcatc ccgacgggtg
     3361 tgatacggac ggacgacgag cggacccacc actacgacag ccagcaccgc ctggtgttcc
     3421 acacgcggat acagcatggc gagccactgg tcgagagccg ctacctctac gacccgctgg
     3481 gacggcgaat ggcgaaacgg gtatggcggc gggagcgtga cctgacgggg tggatgtcgc
     3541 tgtcgcgtaa accggaggag acatggtacg gctgggacgg cgacaggctg acgacggtac
     3601 agaccgacac cacacgtatc cagacggtat acgagccggg aagcttcacg ccgctcatcc
     3661 gggtcgagac agagaacggc gagcgggaga aagcgcagcg ccgcagcctg gcggagaagc
     3721 tgcagcagga agggagcgag gacgggcacg gcgtggtatt tccggctgaa ctggtgcggc
     3781 tgctggacag actggaggaa gaaatccggg cagaccgcgt gagcagtgaa agccgggtgt
     3841 ggcttgcgca gtgcgggctg acggtggagc aactggccag acaggtggag ccggaataca
     3901 caccagtgcg aaacgttcat ctttaccact gcgaccatcg gggccagccg ctggcgctca
     3961 tcagcgaaga cggcgatacg gtgtggagcg gggcgtatga tgagtggggc aaccagctaa
     4021 atgaggagaa cccgcatcat gtgtatcagc cataccgtct gccggggcag cagtatgatg
     4081 aggagtcggg gctgtactac aatcgtcacc ggtactatga tccgttgcag agacggtaca
     4141 tcacccagga ccccattggg ctgaaaggtg ggtggaatct gtatcagtat ccgctgaatc
     4201 cggtgagcag gatagatccg ttggggttat tctcatgtga gagttgtagt cattttatag
     4261 atgatataaa taataaactg gataaccttc cgtttaaccc gacaaaaatg tttacagcta
     4321 gtttaaatac tgctaattca gcaaggctgt atgcaagtgg catgacaaaa ttcggtataa
     4381 gtgttgctgc cgatggaacg atcattgctg ccccagtcgg tgcagcttct atggctttag
     4441 gggcgtggaa tataaaatct gcgacagctg catataacag agcaaattta caattatcag
     4501 aatcacttca ggaaaatggt agagataggt gttggagaaa tttattgggt attttacctt
     4561 atggtgagca gtttgatgac cctggtgaac catcaatatt agacgtttat ggtgaaaaga
     4621 ttgagaatat caaagattct ccaatgaagt tttttgaaga gcttgggacg ctggggttgt
     4681 aatgtgtatt tttaatgata tttttatgtt tgtgtttatt gttagtagta tacttgtaac
     4741 cgtgcatttt gtgtattctt tattttttaa aagtggaaag gtaaaagata aggtaaaaaa
     4801 atggattaaa gtaatgtgtg atttattttg ggggatagga tagtggtgtg atatggataa
     4861 atataaatta cagtggctgg catatttcga cctgacgccg gagtagataa aaaaacagac
     4921 aggattggag tatagcaagc gtctgacaat ctctcgggac agtagtatga tgaagaggcg
     4981 gggttgtact ataaccgtaa ccggtactac gatccgctaa agggcaagta tatgactcag
     5041 ggaaagatca tcagaagttc actttttgta ctaaataatt cgcattttat gtttaaaaat
     5101 tgagatattc ctcattacct gaagctgttt ttttattgct tatacataat caaatactcc
     5161 ttacataatt aaggagaaca aaatggaaat taaaaaattg atggaacatt ttttgaagca
     5221 atatggtgat tttgaaaatg gcattcctgt tcacgatacc attgccagag ttgtatcctg
     5281 tatcagtcct gcaaaatttc atgagtgctt tattaactgg atgcgtgact gccattcttc
     5341 agatgataaa gacgtcattg caattgatgg aaaaacgctc cggcactctt atgataagag
     5401 tcgtcgcagg ggagcgattc atgtcattag tgcgttctca acaatgtaca gtctggtcct
     5461 cgggcagatc aagacggatg agaaatccaa tgagattaca gccattcctg aacttcttaa
     5521 catgctggat attaaaggaa aaatcatcac aactgatgcg atgggttgcc agaaagatat
     5581 tgcagagaag atacaaaaac agggaggtga ttatttattc gctgtaaaag gaaaccaggg
     5641 gcggctaaat aaagcctttg aggaaaaatt tccgctgaaa gaattaaata atccagagca
     5701 tgacagttac gcaat
//
LOCUS       DAJVRF010000197         5615 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJVRF010000197 DAJVRF010000000
VERSION     DAJVRF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.197"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            685..2970
                     /gene="nrdA"
                     /locus_tag="OQN13_003720"
     CDS             685..2970
                     /gene="nrdA"
                     /locus_tag="OQN13_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCS1616956.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3204..4334
                     /gene="nrdB"
                     /locus_tag="OQN13_003721"
     CDS             3204..4334
                     /gene="nrdB"
                     /locus_tag="OQN13_003721"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCS1616957.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4334..4588
                     /gene="yfaE"
                     /locus_tag="OQN13_003722"
     CDS             4334..4588
                     /gene="yfaE"
                     /locus_tag="OQN13_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCS1616958.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4665..5291)
                     /gene="inaA"
                     /locus_tag="OQN13_003723"
     CDS             complement(4665..5291)
                     /gene="inaA"
                     /locus_tag="OQN13_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCS1616959.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRNKAINHDFRQLENIWSQSPKRTGSR"
     gene            5470..5615
                     /locus_tag="OQN13_003724"
                     /pseudo
     CDS             5470..5615
                     /locus_tag="OQN13_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1430 a         1467 c         1336 g         1382 t
ORIGIN      
        1 catcatttta tataagacgg catatttaaa cgcattatac aatatgggtt atctttcttt
       61 tgtaaattgt ccaacccgat gcaatggcta ttttgttatt atttgatgaa taatatcagt
      121 gcgtcataat tcaagttaat aaccttaagg gataccagtt atatttaaac taaattatag
      181 taatgaataa ttttcttata atataaggta aattaacaaa atggcttcgc atttaacaat
      241 aaccgaatag aaaacaacca tttcgacatc aacaatcgct tacattcgct tatatattga
      301 ccacaactga tacatcagat tatgtgatga ctcgtgctta gatcaatttt tgcaatcatt
      361 aacaaaaaga ttaataaacc atctatatca atttatccaa cctattctgc cgttcaagaa
      421 atcgccgaac agttattttt aacaaatttt tctcttccca ttgactttcc cggacacctt
      481 gtctgaccta aggtgcgcga aagccacttt ttccttcctg agttatccac aaagttatgc
      541 acttgcaaga gggtcatttt cacactatct tgcagtgaat cccaaacata ccccctatat
      601 atagtgttct aagcagcttc ccttactaca ggtagtctgc atgaaactat tgcggaaaga
      661 attccaaaaa caggtacgac atacatgaat cagaatctgc tggtgacaaa gcgcgacggt
      721 agcacagagc gcatcaatct cgacaaaatc catcgcgttc tggattgggc ggcagaagga
      781 ctgcataacg tttcgatttc ccaggtcgag ctgcgctccc acattcagtt ttatgacggt
      841 atcaagacct ctgacatcca cgaaaccatt atcaaggctg ccgcagacct gatctcccgt
      901 gatgcgccgg attatcagta tctcgccgcg cgcctggcga tcttccacct gcgtaaaaaa
      961 gcctacggcc agtttgagcc gcctgcgctg tacgaccacg tggtgaaaat ggtcgagatg
     1021 ggcaaatacg ataatcatct gctggaagac tacacggaag aagagttcaa gcagatggac
     1081 acgtttatcg atcacgaccg tgatatgacc ttctcttatg ctgccgttaa gcagctggaa
     1141 ggcaaatatc tggtacagaa ccgcgtgacc ggcgaaatct atgagagcgc ccagttcctt
     1201 tatattctgg ttgccgcgtg cctgttctcg aactacccgc gtgaaacgcg cctgcaatat
     1261 gtgaagcgtt tttacgacgc ggtttccaca tttaaaattt cgctgccgac gccaatcatg
     1321 tccggcgtgc gtaccccgac tcgtcagttc agctcctgcg tactgatcga gtgcggtgac
     1381 agcctggatt ccatcaacgc cacctccagc gcgattgtta aatacgtttc ccagcgtgcc
     1441 gggatcggca tcaacgccgg gcgtattcgt gcgctgggta gcccgattcg cggtggtgaa
     1501 gcgttccata ccggctgcat tccgttctac aaacatttcc agacagcggt gaaatcctgc
     1561 tctcagggcg gtgtgcgcgg cggtgcggca acgctgttct acccgatgtg gcatctggaa
     1621 gtggaaagcc tgctggtgtt gaaaaacaac cgtggtgtgg aaggcaaccg cgtgcgtcat
     1681 atggactacg gggtacaaat caacaaactg atgtataccc gtctgctgaa aggtgaagat
     1741 atcaccctgt tcagcccgtc cgacgtaccg gggctgtacg acgcgttctt cgccgatcag
     1801 gaagagtttg aacgtctgta taccaaatat gagaaagacg acagcatccg caagcagcgt
     1861 gtgaaagccg ttgagctgtt ctcgctgatg atgcaggaac gtgcgtctac cggtcgtatc
     1921 tatattcaga acgttgacca ctgcaatacc catagcccgt ttgatccggc catcgcgcca
     1981 gtgcgtcagt ctaacctgtg cctggagata gccctgccga ccaaaccgct gaacgacgtc
     2041 aacgacgaga acggtgaaat cgcgctgtgt acgctgtctg ctttcaacct gggcgcaatt
     2101 aataacctgg atgaactgga agagctggca attctggcgg ttcgtgcact tgacgcgctg
     2161 ctggattatc aggattaccc gatcccggcc gccaaacgtg gagcgatggg tcgtcgtacg
     2221 ctgggtattg gtgtgatcaa cttcgcttac tacctggcga agcacggtaa acgctactcc
     2281 gacggcagcg ccaacaacct gacgcataaa accttcgaag ccattcagta ttacctgctg
     2341 aaagcctcta atgagctggc gaaagagcaa ggcgcgtgcc cgtggtttaa cgaaaccact
     2401 tacgcgaaag ggatcctgcc gatcgatacc tataagaaag atctggatac catcgctaat
     2461 gagccgctgc attacgactg ggaagcattg cgtgaatcaa tcaaaacgca cggcctgcgt
     2521 aactctacgc tttctgccct gatgccgtcc gagacctctt cgcagatctc taacgccact
     2581 aacggtattg aaccgccgcg cggttacgtc agcatcaaag cgtcgaaaga cggtattttg
     2641 cgccaggtgg tgccggacta cgagcacctg cacgacgcct atgagctgct gtgggaaatg
     2701 ccgggtaacg atggctacct gcaactggta ggtatcatgc agaaatttat cgatcagtcg
     2761 atctctgcta acaccaacta cgatccgtca cgtttcccgt caggaaaagt gccgatgcag
     2821 cagttgctga aagacctgct caccgcctac aaattcgggg tcaaaacgct gtattatcag
     2881 aacacccgtg acggcgctga agacgcgcag gacgatctgg taccgtcaat ccaggacgat
     2941 ggctgcgaaa gcggcgcatg taagatctga tattgagatg ccggatgcgg cgtaaacgcc
     3001 ttatccggcc tacggctcgg tttgtaggcc tgataagacg cgccagcgtc gcatcaggct
     3061 ccgggtgccg gatgcagcgt gaacgcctta tccggcctac ggctcggatt tgtaggcctg
     3121 ataagacgcg ccagcgtcgc atcaggcaca ggatgcggcg taaaatgcct tatccggcat
     3181 taaactccca acaggacaca ctcatggcat ataccacctt ttcacagacg aaaaatgatc
     3241 agctcaaaga accgatgttc tttggtcagc cggtcaacgt ggctcgctac gatcagcaaa
     3301 aatatgacat cttcgaaaag ctgatcgaaa agcagctctc tttcttctgg cgtccggaag
     3361 aagttgacgt ctcccgcgac cgtatagatt accaggcgct gccggagcac gaaaaacaca
     3421 tctttatcag caacctgaaa tatcagacgc tgctggattc cattcagggt cgtagcccga
     3481 acgtggcgct attgccgctt atttctattc cggaactgga aacctgggtc gaaacctggg
     3541 cgttctcaga aacgattcat tcccgttcct atactcatat cattcgtaat atcgttaacg
     3601 atccgtctgt tgtgtttgac gatatcgtca ccaacgagca gatccagaaa cgtgcggaag
     3661 ggatctccag ctattacgat gaactgatcg aaatgaccag ctactggcac cttctgggcg
     3721 aaggcaccca caccgttaac ggtaaaaccg taaccgttag cctgcgcgag ctgaagaaaa
     3781 aactgtatct ctgcctgatg agcgttaacg cgctggaagc gatccgcttc tacgtcagct
     3841 ttgcttgttc ctttgccttt gcagaacgcg aactgatgga aggtaacgcc aagattattc
     3901 gcctgattgc ccgcgatgaa gccctgcacc tgaccggtac tcagcatatg ctgaatctgc
     3961 tgcgcagcgg cgcggatgat ccggagatgg cggaaattgc cgaagaatgt aagcaggagt
     4021 gctatgacct gtttgtccag gcggcacagc aagagaaaga ctgggcggat tatctgttcc
     4081 gtgacggttc gatgattggt ctgaataaag atattctctg ccagtacgtt gaatacatca
     4141 ccaatatccg catgcaggca gtcggtttgg atctgccgtt ccagacgcgc tccaacccga
     4201 tcccgtggat caacacctgg ctggtgtctg ataacgtgca ggttgctccg caggaagtgg
     4261 aagtcagttc ttatctggtc gggcagattg acgcggaagt ggacaccgac gatttgagta
     4321 acttccagct ctgatggccc gcgttaccct gcgcatcact ggcacacaac tgctgtgcca
     4381 ggatgaacac ccttcccttc tggcggcgct ggaatcccac aatgttgcgg ttgagtacca
     4441 gtgccgcgaa ggttactgcg gctcctgtcg cacccgactg gtcgcaggtc aggtggactg
     4501 gattgccgaa ccgttagcct ttattcagcc gggggaaatt ttgccctgtt gttgccgggc
     4561 aaaaggcgat attgaaatcg agatgtgaat tggttgtagt gccagataca acgcttatac
     4621 gtcttatctg gcctacaacg attacatggc gtagtaatac gctttcacct gctcccagtc
     4681 cgctttgggg attggctcca gatattttcc agctggcgga agtcatgatt aatcgcttta
     4741 ttgcgacgca agcggcgacg acttttctcc aggtcaagaa aaccagcttc tgctttacct
     4801 tctgttttca catagatatg gcgaacataa caacagccat gctgacgatt aacgctatgc
     4861 attttcttaa acgccagcgc cactgctttc aacatggctt gccgtacttc gtcagaatag
     4921 ggcgatactg catgccgggc ataccagtca gcaatgctga tgaaccccgc catatcttca
     4981 gtcaccagca acgcgcgcca ttcaccctca attttcaccg cttcaccaaa aacgatcttc
     5041 ggcacgatga caccggcccg ttccagttct ttaattaccg caacctcacg gacaatcgtt
     5101 ggtcggccga acggataacg tacggaatga aacagatgat gcgtcatgcg ctttacatac
     5161 agctttttgc cgttgcgctc gacgcattgc accccgctca taccattacg gcgatagtta
     5221 ggctcttcaa cccagtcgcc ctctgttgcc caccagtgat taaattcgtc gtactttgct
     5281 gaaactgcca taccctatcg cctgtcattt ttattaacga caatgactat aggtggttac
     5341 ctgaggaaaa tcttaatgaa acgtgtcgta ttaatgaatt tcttttgccg ctactagtgt
     5401 gacaccgctt ttgcccttca tttgcagcgc atagattcca ccagccacga ccactaatag
     5461 tgcgcccagc agataaaatg tgaatttaat caggtggata cagcgatgaa ttttattcga
     5521 cagggattag gcatcgcctt attaccagag ttaacgctga aaaccattgc aggtgaattg
     5581 tgttccgttc ctcacgaacc aaccttctat cgaca
//
LOCUS       DAJVRF010000198         5591 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJVRF010000198 DAJVRF010000000
VERSION     DAJVRF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.198"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..513)
                     /locus_tag="OQN13_003725"
     CDS             complement(<1..513)
                     /locus_tag="OQN13_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCS1616960.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="OQN13_003726"
     CDS             complement(759..1211)
                     /locus_tag="OQN13_003726"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1616961.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="OQN13_003727"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="OQN13_003727"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCS1616962.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="OQN13_003728"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="OQN13_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCS1616963.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="OQN13_003729"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="OQN13_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="OQN13_003730"
     CDS             complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="OQN13_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCS1616964.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
BASE COUNT         1329 a         1463 c         1455 g         1344 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc atcatcgcct
     5221 tcaggtaatg tccatatgca atgcatatgc tctggcaaaa cgacccaggc gttgatttca
     5281 aaaggcctgt ctcgcttaac tttaataatg gcgttacgga gcatctggta ctgggtggtc
     5341 aagagttggc ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg
     5401 taataacgac gatattcaga catggcgact cctttttctc aacggtaaga agaaaaggaa
     5461 aggtcggcaa tgaggtgatg gaacagggca agattacgaa agcccgctcc cctcaaggac
     5521 tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
     5581 gaatacctta t
//
LOCUS       DAJVRF010000199         5551 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJVRF010000199 DAJVRF010000000
VERSION     DAJVRF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.199"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..736)
                     /gene="envY"
                     /locus_tag="OQN13_003731"
     CDS             complement(<1..736)
                     /gene="envY"
                     /locus_tag="OQN13_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCS1616965.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLA"
     gene            complement(919..1809)
                     /locus_tag="OQN13_003732"
     CDS             complement(919..1809)
                     /locus_tag="OQN13_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCS1616966.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(1810..4782)
                     /gene="nfrA"
                     /locus_tag="OQN13_003733"
     CDS             complement(1810..4782)
                     /gene="nfrA"
                     /locus_tag="OQN13_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HCS1616967.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(4769..5509)
                     /gene="nfrB"
                     /locus_tag="OQN13_003734"
                     /pseudo
     CDS             complement(4769..5509)
                     /gene="nfrB"
                     /locus_tag="OQN13_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
BASE COUNT         1255 a         1462 c         1466 g         1368 t
ORIGIN      
        1 tggcgagata attcaacggt gtcaggccgt aaaacgcctt aaaaacagag ataaagtacg
       61 acgtgctgct atagccacat aattgcgcca cctgagtgat atttttgtta tccatcaata
      121 acatctgtac ggcgtaacgc atacgacact ctgtgacaat ctggctatag ctgatatttt
      181 cgttttttaa tttctttttg agcaggctgg ggcttaaaca tagcgaactg gcgacaattc
      241 gcagattcca gtaatgctga atatcgcttt gaataatgcg gcagacgctg tcgcggacgc
      301 tgctgcgtaa gatatacatc agtagggcaa taaaccgcga ttgctcaaga aagttagaca
      361 atacggtaaa aagcaatgcg cgcgtcaact ccgcttcgca gggtttgctg ggttgctgct
      421 tgctgtggtt ggcggctagc cggaacactt ccggtgtacg gctatgacaa gcgataaccg
      481 gggttgccag ccgcgaccag ggctttacac aggttaagtt cttatttaaa aacaacagat
      541 aatcgttgat gatgttacgg ttgatgtggg tgattaatac gtgatttaac gttgagagtt
      601 caatgacgtt attgttgcac gcgaaggcgg ccaggtagtt tttcggaagg gtaaacgtag
      661 catggttatt gacgcttacc tctacctctt tttcggtcag gatcaccacg caaggttcac
      721 tgctgctcaa ttgcatttcg cactcctcag atatcagaaa ctccgctcaa aggatctatg
      781 cttcctgcat gagtgatcgg cccgttcgcc gataacgatc ttctttcttt agcacgcttt
      841 ttagcaatta atcttgatgg aattctgacg agagcgaaag aggtaagcca ggtcgtaccc
      901 gacttacctg gaggagattt aatactcgag aatgccgtgc gcgacgggca aatagcgcag
      961 agagaaatag agagtgtctt taccgcaaga ggaacgtttc gctaacagcg aggcgatctc
     1021 tgcatccggt ttcggtttcg ctgtaaaggt tttgcctttg ccggcgacaa acagttcata
     1081 aacaatgccg cttgctgggg cggagctttg gcaatcggcg ctggcctgta gataacgttc
     1141 acgctgttca gcggtaagtt tatccacgcc gctgccatcc tgtacccaga cattaactcc
     1201 ggttgcttta acttgttcca gcagttggcg atagccatct ggcgacatgt ttccggcgaa
     1261 aaaactactg atataaaccg gttttgctga aacatcgcta atcagccgct gcgcgttgtt
     1321 taaccatgtt agcaaaggct gacgggcagc ttcgctgcgc cagttcaggt cgtcaatttc
     1381 cgcgctgatg taccagccat ccggcgttat gccaggcgcg gcgctccata atctggcttg
     1441 ctggagatcg gcagccagca ggcgattaag atagctttcc agcgctgcgg acgactgttt
     1501 ctggtgcata aaaaattccg gatcggcgtt cagcccgaca atcagtttca ggccagcctg
     1561 ttgcgcagct gcggcacgct taaacaataa cgtgcgctgt tctggctggg taaatgcatc
     1621 gccgtaacgg gtccattgca aaaccagggt atcgaagcct tgcaaacgta actgactcat
     1681 cagcccctgc cactgggtat cggtaacctg actatctcgg ttttgtggtt gccagataat
     1741 acctttcatc gcaaaggaaa aagggctgac caaaagcagt gtcagcaata cgaaaatgaa
     1801 cttacgcatt taccagtgca ctccaatggt gagaaacgcg ttgttgcgct ctccgttacg
     1861 ttgattaatc gccttaaagg tatgttgata ctcgacgccg agactgactt tgtgcggcca
     1921 ggcgtcgtag tgcgtctcgc cggtccagat attccagcgg accccgactc cgccaagctg
     1981 cgcgccctga gtgcctttat cacgatagcc gttgtcctga acgtgagcgt aaggctcaat
     2041 agtctgtccg ttagctacct tctgatgcca gctgacgcga taatctgccg tccacgcctg
     2101 aatatcctgg cggatatatt gcgccgcatc gaggtacagg ttttgggcaa accagcctga
     2161 accgttcggg tgccattcgt cgctgtattt gccgccatta aagaatgagg cgctggcgcg
     2221 cagcatggta tcggatgcgc cattttgccc gttcagcggc aactgctgtt cgacggcgag
     2281 gaaaaagatc tgatcgcgca gcggcttcca gcgcagaccg gtgccggaca tcggattttt
     2341 caccggcatc atcaccccgt tttctccggt atcggcaaag acgcggctat aaactgagag
     2401 caggtcgcct tccagcagca tattgcgtcc gatgcggtac tcggcttcca gttgcccgta
     2461 gctacgatag cttttccctg gcgctgcgcc gccaacatta ttgttagcgg tactcattgc
     2521 cccggaacgc aagccgatag aagaatcgaa actgaacgtc cagcggcgac cgacctcctc
     2581 atgcaaacgg cggaaattga agcgttgttg attttgctct ggggtcagtg gggttatcag
     2641 agcctgatta tcaatgtcat caatcaccag ccgggcgtag tgctgcgtcg caggcatgtc
     2701 atccagacgc tggttcacgt aggccagttg tcggatcagt gccggatcgt ccggaagcct
     2761 tttatgcgcc tgttcgagca tttcccgcga ctgtgcgata tcaccgctat cccacaaggc
     2821 gtaaccaagc gctgcctgga tgttgctatt attcggttcc agttccagcg cggcgcgcaa
     2881 atcactcacc gcggccggga cattatgacg ttggcgataa attgtcgccc gcgcaacgta
     2941 agcgttggca gaaggcgcaa tattgattga gcgcgtgaga tcgttcagtg cgagttccgg
     3001 ctgaccagga atgtaacgtt gtgcatgcag ccaccagtag agggcattgc ttcccagtcc
     3061 acgttgttct gcctgttgta gccagcgatc gcgagccgca ccatttcctg ccgcctgggc
     3121 ggtattggca gcagcaagca gatcctcatt gctcatgtcg tgaagactga ttttctgcca
     3181 ggccgccagt gcggtggcgt agtcctcaac ctgatacgcc tgataggcta ccgcacgatg
     3241 ttgccaggcg ctcggttgtc gttgttcggc ctgaagccat gcatacaacg ccacaccggg
     3301 tagcgtgtcc cgataacact ttgccagacg gttccaggcg gcagcatcgt aggaaggcga
     3361 catatcgccc agcaagcgaa ctattgccgg gcaattatct gcaatacccg gcaactgact
     3421 ttgccactga cgttgctccg ccagcggtaa gggtttcgat aaaatcgcca ccttcgccgg
     3481 cgttgccagg taaggatgac tttccagcag agacgccagt cgcgccatta aagtctggct
     3541 gacacgcgca tcgccctgga aaggatagcg ttgcagcaat aaatcggcag cttcgcgtga
     3601 ctgctcgttc tgcatcagtt gccaggtcag ttgatccagg cgggtaagat ttgccggttc
     3661 ttgctgatac agcaatcgtg ccagacgcag agattcagcc ttgttacggg tcgccacgct
     3721 gacagcataa cgctcctcaa gcatttcatt ggcggggagg gtggcgagca gtttttgcgc
     3781 tgcgtcgtac tgaccttctt ttaacagcac cggtagcgtc gcgccaacaa catactggcg
     3841 gttatcggca aactgcaccg tataattcgc caatgcctga acggggttgg cgctgtattt
     3901 agataacaga tagagccaac ttttctcttg tgcgtccgtg gtaaacagcg gcttattttc
     3961 attgagataa tgctggaggc gtgctttttc gccacgataa gccagcgcgg tcgcgtaagt
     4021 aatatatgac tgaggatcgg tgaagatccc ctgtgattgc agtgctagga tccgatcgtc
     4081 cagctgcccg gcaagaagca cgtcaaacca ctgacggcgt tctgccgcgc ttaatgtgtt
     4141 ctgctggcgt gcttcattgt atagcgtatc tgcctgggac cattgtttca ggtagattgc
     4201 ccgttgcagc agatcggttc gcagcgtttt tccttccggc gatgcagcaa acgtcgcatc
     4261 gttcagttgc gctctggcga caggtaactg tgcaagccgc agggcattct gcccgacttc
     4321 actgcgacaa cgcagggtcg gcgcagcatc gcacgctttt tgctgggcaa gcagttcttc
     4381 aacagttgta acgcttttca cttcaaccgg aatagccgcc agactgcgct caagtcgggc
     4441 atctcctggg tgacgtttca gttgatcctc aagcaacagc cgcgcccggt catcatgacc
     4501 aaaatggcga taggcttccg caaggtataa agtcagcgga atattatccg gcacctgctg
     4561 gtgtatatat tcaaattcgc ggatggcggt tgcttcgtcg ttatttttct gtgccttcag
     4621 cgccttatcg agacggggat aaataacaaa atggcgataa tcgctcagcc ccagctcttc
     4681 tgcgctggtg ccgatattgt ctgcgagtgc gctggtactc aataaagacg tcagcagtaa
     4741 accagaccat ccgatgacgc gattaaggtt attctccttc attttcggac tccagttgcg
     4801 caacctgttc tgtgtttaaa cctgctttga gtaatagtga ttgcatcgaa acttgtaatt
     4861 cgcgttgaat tgtcaggacg cgatccaacg tttcctggct gataacgcct tcggtgacca
     4921 aaaacttgcc gagcggcaga gaactgcgtt catggcgcaa taacaacacg ttaattgctg
     4981 aacgattaat atgaccgagc gtggtcagta tttcggcgaa caggaactga tgcggcacat
     5041 attgccgcca gatttcaccg gcctgctgtt ccgtgagcca ctgatgctga accgcattgt
     5101 acaacattgc ccgcggatcg tgaccgcgtc ggcgtgcata ccagtgacgt aaccccgtga
     5161 caatttgtcc ccgcagaaca atgaagtaac gcactttgcg tccgacttta cgcgtcaggg
     5221 ccgccagcga aaccgggtca ataccatctt cactgccgac aattaactca tcattttcca
     5281 gacgcagcgg cagtaccgca taatgcagcg ccacggaggc cggcatttcg gcaatcagcg
     5341 aggaagggat ctgccaggca tcgatggatt cccacgccac gccgttttgc tctgccagcg
     5401 cctgtgccag ctgctcggcg ctaatcagcc cctgcatcag cattgaaccg cccaagcgta
     5461 gaccttcgac gcgattacgc agtgctgtat cgagttgttc ggtaatgact ccaacttatt
     5521 gatggtgttt tatgttcaga taatgcccga t
//
LOCUS       DAJVRF010000200         5526 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJVRF010000200 DAJVRF010000000
VERSION     DAJVRF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.200"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="OQN13_003735"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="OQN13_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCS1616968.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="OQN13_003736"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="OQN13_003736"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCS1616969.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="OQN13_003737"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="OQN13_003737"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCS1616970.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="OQN13_003738"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="OQN13_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCS1616971.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1361 a         1325 c         1503 g         1337 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgtaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccg
//
LOCUS       DAJVRF010000201         5451 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJVRF010000201 DAJVRF010000000
VERSION     DAJVRF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.201"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..255)
                     /gene="minE"
                     /locus_tag="OQN13_003739"
     CDS             complement(<1..255)
                     /gene="minE"
                     /locus_tag="OQN13_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCS1616972.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAE"
     gene            complement(259..1071)
                     /gene="minD"
                     /locus_tag="OQN13_003740"
     CDS             complement(259..1071)
                     /gene="minD"
                     /locus_tag="OQN13_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCS1616973.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(1095..1790)
                     /gene="minC"
                     /locus_tag="OQN13_003741"
     CDS             complement(1095..1790)
                     /gene="minC"
                     /locus_tag="OQN13_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCS1616974.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            2310..2678
                     /locus_tag="OQN13_003742"
     CDS             2310..2678
                     /locus_tag="OQN13_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCS1616975.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(2786..3187)
                     /locus_tag="OQN13_003743"
     CDS             complement(2786..3187)
                     /locus_tag="OQN13_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCS1616976.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            3429..3722
                     /locus_tag="OQN13_003744"
     CDS             3429..3722
                     /locus_tag="OQN13_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCS1616977.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            3794..4453
                     /locus_tag="OQN13_003745"
     CDS             3794..4453
                     /locus_tag="OQN13_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCS1616978.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFISAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            4545..4991
                     /locus_tag="OQN13_003746"
     CDS             4545..4991
                     /locus_tag="OQN13_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCS1616979.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(5198..>5451)
                     /gene="hlyE"
                     /locus_tag="OQN13_003747"
     CDS             complement(5198..>5451)
                     /gene="hlyE"
                     /locus_tag="OQN13_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HCS1616980.1"
                     /translation="FFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYV
                     DYDDLMLSLLKEAAKKMINTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         1377 a         1218 c         1299 g         1557 t
ORIGIN      
        1 ttctgcttcc ggtaaggtca cgttcagctc aagaatagaa atatcgccat ctttttgctc
       61 aagctgtacg gttaccatct caggatcaat ttgtacgtat ttacaaatga cctcaagaat
      121 atctttacgc aactgcggca gataatgcgg ttctgcatcg ctgcgacggc gttcagcaac
      181 aataatctgc agccgttctt ttgcaatgtt ggctgtgttt ttcttccgcg agagaaagaa
      241 atcgagtaat gccataactt atcctccgaa caagcgtttg aggaagcctt tcttctcttc
      301 ttcaatgaag cggaaaggac gttcttctcc caacagacgt tctacggtat ctgcgtaggc
      361 tttacccgca tcggcgttaa tgtcgagaat gaccggttca ccctggttag aggcgcgtaa
      421 tactgattga tcctctggga tcacgccgac gagtttgatg cgcaggatct ccagcacatc
      481 ttccatgctc agcatgtcac ctctgcttac gcggcctggg ttatagcgcg ttaacagcag
      541 gtgctcttta ataggctctt cgccattttc tgcgcggcgt gatttcgacg ccagaatgcc
      601 taaaatacgg tcagagtcgc gtactgagga gacttccggg ttggtggtaa taatggcttc
      661 gtctgcaaaa tagagtgcca ttaacgcacc ggtttcaatc cctgccgggg agtcacaaac
      721 gataaattca aaatccatcg ctttcagatc atcaagaact ttggcgaccc cttcacgggt
      781 gagggcatct ttatcgcgtg tttgcgatgc cggaagaata tagagatttt cagtacgctt
      841 atctttaatt aacgcctgat ttagcgttgc atcgccctga atgacgttga cgaaatcgta
      901 aacgacccgg cgttcgcaac ccataatcag gtcgagatta cgcaggccga tatcaaaatc
      961 tatcacgaca gttttctttc ccttctgggc caaaccagtg gcgatggccg cgctggaggt
     1021 tgtcttacca accccccctt tgcccgaagt aacaacaata atgcgtgcca tagaaattcc
     1081 ttgttaaaaa gggatcaatt taacggttga acggtcaaag cgttttcgac taactgcagt
     1141 cgcgccgctt tgccataaaa ttctgctggg atttgatcac tcagccagta ttcacctgcg
     1201 atggacacca attccgccat caggttcgta caaaatattt gcgtttcccg gtcaccactt
     1261 gcccctgcca gcgcacgacc gcgcatcatg ccatagacat gaatgttccc atcggcaatc
     1321 aattcggccc cagcgctaac gtggcttgta acaatcagat cacattgtgg agcataaata
     1381 cgctgaccgg aacgcaccgg ggtatctatt aaacgcgttt ttgtgaccgg cgtggtattt
     1441 tgcgctggag cctgtggtgc gggagctgga cgtggcgctt tttcctttcc ttccgtcagg
     1501 ataggcagcc ccattttttc aatttcggct ttaagttgcg cgtctttgca gccacttacg
     1561 ccaataaccc gcaaaccggt tgccgaaacc gccttatgca tcgctgacca gtttaccggg
     1621 tcttccagtg cactgacgtt gagtacaacg ggggcatgtt ttaaaaatgc gggggcctga
     1681 gcgattttgt cttccagcgc ctgatggata accttaggtt ctgcctcatg cagatgaacc
     1741 acagataaag tgaagctact gcctttaagc tcgattggcg tgtttgacat cctggcctta
     1801 ctcaattagc tattaatcat cgccagcgcg cgatgatgtt ccgaagacta taaggcatgt
     1861 tatagtctgg attatattga ggcaagtcac cctcccattt attcagagta aaagtctatt
     1921 ctgtgataaa tggcgctgat tcatagctta aaaaataccc ttgtcaatca acccattgcc
     1981 gccgtacttt tgattcttct tatttacgct tcctttttcc gcaccctaat cgtgacagca
     2041 tctccagacc tgtttaaata tccagcattg aggcaagtgt attttttcac cacgccaggc
     2101 tttcttaact ttccattttt tgcagcatca ttaaagaaca gatacccgtt atcttaatat
     2161 ccgccacaga attgctctat catagaaacc gcagaagacg tctgatactt ctgtcaggtc
     2221 atcgtaactc gtccaggtca gcggttttga ttacggattg atgacgaaat gagcataagt
     2281 gttgtacggt tggattatca ggatgtgcta tgaatatgat gcgtattatt tatatcggat
     2341 tgtcaggtgt ggggatgatg ttctcatcaa tggcgtctgg acatgacgct ggcggacttc
     2401 aatctccggc gtgcggcgtt gtttgcgacc cgtatatttg tgtgaactca gatggcattt
     2461 ccccagagtt aacaaggaaa tatctcggcg aaaaagccgc tgaaaactta caatcattac
     2521 aaggctacga tcccagcgaa tttacattcg ctaacggtgt attttgcgat gttaaagaaa
     2581 aattatgtcg tgatgatcgt tattttggtg tagatggtaa gcggagtgga aaaatcaatc
     2641 aaaccaccac aaagatgtta tttatgtgtc gtgagtgatg ccttgtttac attatgttct
     2701 ctgctattcg tcattaacct cttgagcgac agagaggagt ggggcgttaa cgtctatgat
     2761 ggggatagtc cccgtcggtt gacatttact taatttgaat atcgacgtta tattttttag
     2821 ttttgttttt acgggcgtcg tttcgagttt gcagcactcg cagctcatat ttgccagaag
     2881 cagggagtga gtattgtccg tgactatcga gttctgggga atatctggat aaatcaacag
     2941 aatcatcgat tcctggcccg aagagataag tatcagcgcc ttcattagag atactcacat
     3001 gcactttctg accttttttg gcatagaagg tatatgtatc gtaatcgtat ccctttattt
     3061 cgccggagta ctgagcgctg ctatggcctt tgctgaactc aacattgaca tttttacccg
     3121 cagcaaagct cgtggaggtt agcagtgcaa gcagcaatac agccttactg atgctcttga
     3181 ttttcattgg tagtctcctg ttcagaatga gtacgatgaa ctgttataat ataacaatcc
     3241 cttacgggag aattccagtg gcttttctgc gttcggtaaa atcccggcgc ggcatttttt
     3301 gccgggggga ttacttataa aggggcatgg actcaacaaa ccgcagaaat ctacagctgg
     3361 ttggcttgtg gcgcgggttt cattgatgtt agcttatgcc aaaacctggc atcttaaaga
     3421 gtaaaagtat gttttgtgtg atttatcgaa gcagcaagcg tgaccagacc tatttatatg
     3481 tcgaaaaaaa agacgatttt tcgcgtgttc ctgaggaact gatgaaaggt tttggtcagc
     3541 ctcagttagc gatgattctg ccgttggatg ggcggaagaa actggtcaat gctgatattg
     3601 aaaaggttaa actggcatta accgagcaag gttactattt gcagttaccg ccaccacctg
     3661 aagatttgct gaagcaacat ctttccgtca tggggcagaa aacagacgac actaacaaat
     3721 aaccgatatc cggcggtggc attatctttg tcggcgcggg ttttcatatc cacgataagg
     3781 tgaggggaac gttatgtatc aacatcacaa ctggcaaggt gcgctgctgg attatccggt
     3841 gagtaaagta gtctgtgttg gcagtaacta tgccaaacat attaaagaga tgggcagcgc
     3901 agtgcccgaa gagccagtgc tgtttattaa accagaaacg gcactgtgcg atctgcggca
     3961 gccattggcg atcccatccg atttcggttc agttcatcat gaagtcgaac tggcggtgtt
     4021 gattggcgcg acgctgcgtc aggcaacgga agagcatgtc cgcaaagcca ttgccggtta
     4081 cggcgtggcg cttgatctga cattgcgtga tgttcaggga aaaatgaaga aagccgggca
     4141 gccgtgggaa aaggctaaag cgtttgataa ctcttgtccg ctttccgggt ttatctccgc
     4201 ggcggaattt accggcgatc cacaaaatac aacgctgggc ctgagcgtaa acggcgaaca
     4261 acgccagcaa ggtacgactg cggacatgat ccataaaatc gttccgttga tcgcttatat
     4321 gagcaagttt tttaccctca aggccggtga cgttgtgttg acaggcacgc ctgatggcgt
     4381 cggcccgttg caaagcggtg atgagctgac agtcactttc gatgggcatt ctttgacaac
     4441 tcgcgttttg taatactttt tgccgcctga aagcggcggc aaaacttgca tcgctgtgcc
     4501 agactggtta taaggtgcgt tttaacgtaa tggcggaaca cctgatgagc gatgtacctt
     4561 tctggcaaag taaaaccctg gacgaaatga gcgatgcgga atgggagtcg ttgtgtgatg
     4621 gttgcggtca gtgttgcctg cataaactga tggatgaaga caccgacgaa atctacttca
     4681 ctaacgtcgc ctgtcgccag ctcaatatta aaacttgtca atgtcggaac tacgaacgtc
     4741 gtttcgagtt tgaacccgac tgcattaaat taacccgtga aaatctgcca acattcgaat
     4801 ggctgccaat gatctgcgct tatcgtttgc tggcggaagg taaagattta cctgcgtggc
     4861 atccgctact tactggttcg aaagcggcaa tgcatggtga acgtatctct gtgcgtcata
     4921 tcgcagtgaa agaatcagaa gtgattgact ggcaggatca tatcttaaat aaacctgact
     4981 gggcgcagtg ataaatttaa tagatgttga tgggggcttt cttaatatac gtctggatgc
     5041 tcgccatttc gtagccctgt ttttgaaagc gctctgtcat aatgagagtt acccgatacc
     5101 ttattatcaa caggtaactc tcaatcttta aaaaacgtct gtgtttttaa atgtgataaa
     5161 ccttatacct tgagtacatg gagagaataa tcgctgatca gacttcaggt acctcaaaga
     5221 gtgtcttttt accgtgtttt ttctgatact cattacaggt gttaatcatt tttttggccg
     5281 cttcttttag caaagaaagc attaaatcat cataatcaac gtagaatctg gtggtttcag
     5341 tttccgtttt tatctctccg atggcggcta tttcggtggt taatttcaat ttggcggcat
     5401 cgatatcttt attcgcttgt ttaaccgtgt tagacagggt ggtaaagaaa c
//
LOCUS       DAJVRF010000202         5421 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJVRF010000202 DAJVRF010000000
VERSION     DAJVRF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.202"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..115)
                     /locus_tag="OQN13_003748"
     CDS             complement(<1..115)
                     /locus_tag="OQN13_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCS1616981.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLE"
     gene            complement(220..1722)
                     /gene="dtpA"
                     /locus_tag="OQN13_003749"
     CDS             complement(220..1722)
                     /gene="dtpA"
                     /locus_tag="OQN13_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCS1616982.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(2333..2968)
                     /gene="nth"
                     /locus_tag="OQN13_003750"
     CDS             complement(2333..2968)
                     /gene="nth"
                     /locus_tag="OQN13_003750"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCS1616983.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(2968..3663)
                     /gene="rsxE"
                     /locus_tag="OQN13_003751"
     CDS             complement(2968..3663)
                     /gene="rsxE"
                     /locus_tag="OQN13_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCS1616984.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(3667..4287)
                     /gene="rsxG"
                     /locus_tag="OQN13_003752"
     CDS             complement(3667..4287)
                     /gene="rsxG"
                     /locus_tag="OQN13_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCS1616985.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(4291..5349)
                     /gene="rsxD"
                     /locus_tag="OQN13_003753"
     CDS             complement(4291..5349)
                     /gene="rsxD"
                     /locus_tag="OQN13_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCS1616986.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         1441 a         1373 c         1329 g         1278 t
ORIGIN      
        1 tttcgagacg ttttttcatt aaatccacac tgacgagggt aaaatccttt ccgctctcac
       61 gcagggtgat atgggaagcg agagagcagg cacccggttt gtagaacaat ttcatcggta
      121 actccttttg tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaagccgcta
      181 acgaaaagtt agcggcttgt aaaagagttt ccctgaattt tacgctacag cagctttcgc
      241 cgctttgtct gcagcgtcat cctgcgtcat acggtgcagt ttaggcgcgg tcagcagcat
      301 cagtactgca ataatggcag tagcgacacc aatctgcaag aatacgcgac cataaacttc
      361 cagtgacatc agcgggtcgg taacgttatc cggcacagcc atcatacccg caacataacc
      421 accaatcagg tttgcaccgg cagtggtcag gaaccagcta cccataatga agcccatcag
      481 acgctgcgga acgagttgtg caaccattgc cagacccaga ccagagatca tcagttcccc
      541 gatgctctgc aggccatagc ttgcgaccag ccagcttaca gacacgatac cagcgtcaga
      601 cgcgaatttc gcacccagcg gcagaatcag gaacgcacca gaacacatca ccatgccgat
      661 tgcaaacttg gttggcatcg gcagggtatc gcccatcttg ttatagatag cggccagaat
      721 cggactaccg atgatgatcc agaacgggtt cagtgcctga tactgctcag gttctacggc
      781 cagacccaga atggagtgct caacgttacg aatcgcaaag aagttcagtg acgttggcat
      841 ctggctgtac agcacgaaga agataatggc ttcgagcatc aggatgaagg caacgatcat
      901 tttacgacgc gcagcacctt tcatcgcgaa ggcttcttta ccgaagataa ccacgatacc
      961 gaaggcaaca acgcccagcg ccatacgcgc aacttcctga ttgtgcagca gccaggtggc
     1021 gatagcgatc agtgccacaa caccaataat ggtcagcagc aggttacggt agttgatagg
     1081 ctcgaagtct ggttttgaac cgtactgttt aacccagcgt tggcagaagg cgaagttaac
     1141 gatagtgatc agcaggccta caacgctcaa cgcaaacgca acactccagc cgtatttcgc
     1201 ggccagccac ggcgtagcaa tcatagagaa gaaagagccg atgttgacgg acatgtagta
     1261 catggtgaat gcaccgtcca gacgcgggtc gtttttctca tagcatgtag aaagcagaga
     1321 agatgggtta gctttaaaca ggccgttacc gaccgcaata gccgccatac ccatataaac
     1381 gataccggcg tcgtgaccag accaggcaac cagagcataa ccaatcgcca gcacaatagc
     1441 accgagcata attacgcgtt tagtacccag taccttgtca cctaaccagc cgccgatagc
     1501 gaccagacca taaaccaggg cactaaagga agagaaaagg gtgattgaat ccgcttcaga
     1561 catacccagt tgtttaacca ggtaaacagc cataattcct tgtaggccgt aataaccaaa
     1621 acgttcccat aactcaatcg agaagatgag atagaacgcc ttcggttgtt tgaaagcgtt
     1681 caaactgacg ctttcagttg gtttttggtt tgcagtggac acttttacct ctttttttac
     1741 atcccatatt aacgggggtg ctttctcgcg ttatgtctaa aaaacatacg cgcaattgtt
     1801 atagtgggag gggaaacggc aggtaatgtt cactatcttg cactttcaga caagtccttt
     1861 gtaataatct gttacatata actgaggcgg ggaattcaac cattccggga aatgttatcc
     1921 agcgttaaat ttttcaaaaa acgtaatggc agattttttc actgcactga gaggggggtg
     1981 atggcttaac ggcgatatgt tctgctattt agcgttttta ctagtgagat agtccagttt
     2041 ctgaaaaata gccagtgtaa tgttttgtag gtcaatattt gttgcgtgtg taaatttaaa
     2101 ttattttaac ctgattcttg caatgttttt acattgcgac agcatcgtga tcttgatcac
     2161 gaatgaatag aaaatttcgc tattaaaaaa acaattaacc tgcatttttg gttatccgca
     2221 aacggattac ctgatgataa cgctaagcaa tggcatcatt cacgttaaaa agatgtttta
     2281 aaagaggata aagaaaggtt atcaatgggg taatcggtgt tacccctttt cttcagatgt
     2341 caactttctc tttgtattca caaagatctt caataataca agagccacag cggggcttgc
     2401 gggcaatgca ggtataacgc ccgtgcagga tcaaccaatg gtggcagtcg actttaaact
     2461 ctgccggaac cactttcagt agcttttctt ctacctgttc gacgtttttc cccggcgcaa
     2521 attgagtacg attacaaacg cggaaaatgt gcgtgtcgac agcaatagtc ggccagccga
     2581 atgcagtgtt taatacgacg ttggctgttt tacgacctac gccgggcagg gcttcaagcg
     2641 cagcacgatc ttccggaacc tcgccattat gctgctccag taagatacgg caggttttga
     2701 tgatattttc tgctttgctg ttataaagcc caatcgtttt gatataggtt ttaacccctt
     2761 caacgcccag ttcaagcatc gctgcaggcg tattcgccac cgggtaaagt ttcgccgtcg
     2821 ccttattaac actgacatcg gtcgcctgag cggaaagcag tacggcaatc agcaattcaa
     2881 aaggcgaact gaaattaagc tcggtggtgg gatgaggatt gttctcacgc aggcgagtga
     2941 ggatctccag gcgttttgct ttgttcatca gacattccct gtttcaccgt ttggcaatgc
     3001 acgttcggca gctgcttcag tacggcgctt tttcattctt tcatcaatca ggtattttcc
     3061 tgccagcatc agtcccaggc caataaatgc acctggtggc agcatcgcca gcaggaaagg
     3121 ggagtcggtg tggaaaatct ccacgcgtaa tacttttgcc cagctaccta acagcgcatc
     3181 tgcaccgtca aacaatgtgc cattgccgat aatttcgcgt agtgaaccca gcacgaacat
     3241 ggcgcaggtt gcgcccatac caattgaaaa gccgtccagt gccgaaagcg ccggaccttt
     3301 tttggcggcg aaggcttcag cgcggcccac aacgatacag ttagtgacaa tcagcggaat
     3361 aaaaatccct aatgattgat acaggccaaa ggcgtaggcg ttgatcagca tctgtacagc
     3421 gctgaccacc gaggcgatga tcatcacgta aatgggaatg cggatctcgg ctggcgtcca
     3481 gtgacgcagc gtcgaaatgg tcaggttggt cagcgtcagt accagcgtag tcgcaagtcc
     3541 taaacccaga gcgttagtgg cagtggacgt gaccgccaac agaggacaaa ggccgagcaa
     3601 ctggaccagc gcagagttgt ttttccacaa cccctgaaca ataacgtctt taatttcgct
     3661 cacgctttat tctccacagg caggaagttg agaaagttgt gccggtaacg tctgagcgta
     3721 caatccggcg cgttttaccg cattaaccac cgcgcgggga gtaatcgtcg cgccggtgaa
     3781 ctggtcgaaa tcaccaccat ctttcttcac cgcccagtgc gcatcatccg caccactgat
     3841 ttttttaccc gcaaaatggg tgatccagtc agaaaggcgc agttcgattt tatcgccaag
     3901 ccctggcgtt tcgtggtgct ctgtcacgcg cgtgccaagt accgtgccgt taaaatcggc
     3961 tcccaccagc agctgaatcg caccggaata gccatctggc gcggttgctt ccagaacggc
     4021 ggctaccggt ttgtcatcct gtttggcgat gtaaacccga tgctcacctt tacctaactc
     4081 tggcgcagtt accagatagc aactctgtgc cagcgtattg ttatagcgtt cggctggcag
     4141 cacctgatca aataatgcct tttgttgcag actggcctgt tcagcaatcg tcgttttggt
     4201 catctggttg atggccgcag ttaaccctgt tgaacccgct gcaaatagcg ctaacgtaat
     4261 gccgtgtttt cggatagttt tcagcatggt ttaccctttg cgatggccgt agacgcgcgg
     4321 acgcgtgtag taatcgatca gaggaaccgt gatgttcgcc agcaggacgg caaaagccac
     4381 gccgtcagga tagccgccga aactgcggat caaccagact aataagcccg caagcgcgcc
     4441 gaaaataaga cgaccacgat tggtcgtaga agcggtaacc gggtcagtca aaataaagaa
     4501 tgcgccgagc atggtcgcac cagacagcag atgaatttgc ggtgctgcca gtgtttctgg
     4561 tgagaacaac cagcccaacg ttgcgcataa cgccagcgtt actaagaagc tgaggggaat
     4621 atgccagcga atcgctttct gccatagcaa ccacaggccg ccagccagcc aggcgagatt
     4681 tacccattgc caaccagcgc ccgccagaat accgctgtag atcggatatt gcataatctg
     4741 ttcaaccgaa tgaccggcac ggacagaggt tttaaatgta tccagcggtg tcgcctgact
     4801 aatgccatca atacctaagc gtagtgtgtt catatcacca ccactggcgg tatgcccgct
     4861 gaaaataacc tgtatggcgt cgataaaacc agggatgttg accgcaattt catgtggcgg
     4921 taaccagctg gtcatctgca ccgggaagga gatcagtaag accacataac caatcattgc
     4981 cggattaaac gggttttgcc ccagaccgcc atacaactgt ttagcgatga tcaccgcaaa
     5041 caccgtaccc agcacgacca tccaccatgg cgcgaggggg ggaatactta ccgccagcaa
     5101 taagcctgtc agcaatgctg agttatcttt caacgttgcg gctaccgact gcttgcgtag
     5161 tttgagtacg agagcttcgg ctaacagagt actaaccgat gccaacagga tctgaacgag
     5221 agtaccccaa ccaaaaaacc acagttgcgc tgcgattcct ggcacggctg cgagcaacac
     5281 caacagcata atgcgcgatg tctggcgctg gttatgggta taaggggagc tagctattct
     5341 gaataccatt tagtcctcgt ttacaacctt ctgctgggcg gcttttttgg cctgagcgcg
     5401 ggcaatagcc gcggcaactg c
//
LOCUS       DAJVRF010000203         5413 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJVRF010000203 DAJVRF010000000
VERSION     DAJVRF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.203"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="OQN13_003754"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="OQN13_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCS1616987.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /locus_tag="OQN13_003755"
     CDS             complement(2975..3697)
                     /locus_tag="OQN13_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCS1616988.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..>5413
                     /locus_tag="OQN13_003756"
     CDS             3844..>5413
                     /locus_tag="OQN13_003756"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 4 subunit A"
                     /protein_id="HCS1616989.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYGTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITA"
BASE COUNT         1289 a         1355 c         1559 g         1210 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact tggcggttta tggcacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg cca
//
LOCUS       DAJVRF010000204         5296 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJVRF010000204 DAJVRF010000000
VERSION     DAJVRF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.204"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            356..981
                     /gene="gstB"
                     /locus_tag="OQN13_003757"
                     /pseudo
     CDS             356..981
                     /gene="gstB"
                     /locus_tag="OQN13_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(978..2093)
                     /gene="yliI"
                     /locus_tag="OQN13_003758"
     CDS             complement(978..2093)
                     /gene="yliI"
                     /locus_tag="OQN13_003758"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HCS1616990.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKHYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="OQN13_003759"
     CDS             complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="OQN13_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCS1616991.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2800..4125
                     /gene="rimO"
                     /locus_tag="OQN13_003760"
     CDS             2800..4125
                     /gene="rimO"
                     /locus_tag="OQN13_003760"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCS1616992.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4172..5296)
                     /locus_tag="OQN13_003761"
                     /pseudo
     CDS             complement(4172..5296)
                     /locus_tag="OQN13_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
BASE COUNT         1384 a         1331 c         1261 g         1320 t
ORIGIN      
        1 cgctcggcgc ttcaacggtt tgttccgccg cgaatgccgt tggggcaaaa aggaataaaa
       61 atgcagaacc cgctgcaaga ccacgaagga gagaggagta ttgcgtcata aatgccaccc
      121 gagtatccat tcaaaaaaaa tacgttatat cgccgtatga taagttcggt gattaataac
      181 gcacacacaa acttaaagaa accgtaagtt ggtaaagttt ttaaagttta tgcaatatcc
      241 tgccgcttcg ctgcttctgg tgtcattttt ttgccagctg ttgcttaaaa gttaacttta
      301 cttcaccatc ggagtcctgt tcagcctgtg gctgcctgtc ggaaaagggg tgagtatgat
      361 tacgctgtgg ggtcggaata attcaactaa cgtaaaaaag tattgctgac gctcgaagaa
      421 ctggaactac cttatgagca aattctcgcg ggccgtgagt ttgggataaa tcacgatgct
      481 gattttctgg cgatgaaccc taacgggctg gtgccgttgt tgcgtgacga cgaaagtgat
      541 ctcattcttt gggaatcaaa cgccattgtc cgctatctgg cggcacagta cgggcaaaaa
      601 cgcctgtgga tcgactcacc ggcacgtcgt gcggaagcgg aaaaatggat ggactgggca
      661 aaccagacgc tcagtaatgc tcatcgcggg atcctgatgg gattagtcag aacaccaccg
      721 gaagagcgcg atcaggccgc cattgatgcc agttgcaaag agtgcgacgc cctgtttgcc
      781 ctgctcgatg cggaactggc aaaagtaaaa tggttctctg gcgacgagtt tggtgtgggc
      841 gatatcgcta tcgcaccgtt catctacaat ttgtttaacg ttggcctgac ctggacaccg
      901 cgtccaaatc tgcaacgctg gtatcagcaa ctcactgaac gccccgcagt gcgcaaagtg
      961 gtgatgattc ccgttagcta attgcgtggg ctaactttaa gtaattcccc actggactcg
     1021 tcggtgagaa cgtataaata accgtcgggt ccagtgcgaa catcacgaat tcgctgccct
     1081 ctgtccgtta aaatacggcc atcttctgtc actttgtcgc cgttgacgct catcacaatg
     1141 acatctttat ctttcagcgc gccaataaat aatttttgct gccactgggg gaatttatcg
     1201 ctgttataga aggccatgcc gctcacagcg ggcgaatctt tccagtaaaa aacaggttgc
     1261 tcggtcccgg cgacgatctc ccctttcgct tccggtatct taaagcctga atagttgatt
     1321 ccccaggttg ccagcggcca gccgtagtgt ttgccttttt gcgggatatt aatttcatca
     1381 ccaccgcgcg ggccatgttc attcagccac agtgcattac tccacggatt catcgccatt
     1441 ccttgcggat tacgaatgcc ataagaccag atctcggcgc gcgcaccgga ttcctttata
     1501 aaaggattat catccgggat ttcgccctgg tcggtcagac gcaccagttt gccctgtaat
     1561 ttatccagat cctgcgccgt cgggcgctga ttgttttcgc ccagagcaat aaaaagataa
     1621 cctttaccgt cgaataccag ccgcccgcca aaatggttgc cggtagacag ttttggcatc
     1681 tggcggaaga cggtgcggaa gtcggtcact tttgagagat catcacttaa gcggccataa
     1741 cccacagcag ttccggcttt gccatcatcg ccaacttcgg aataacttaa ccagatgcgg
     1801 cgagactgag caaaatcagg cgctaaaacc acgtccagca ggccgccctg cccgtgcgcc
     1861 caaacgtccg gaactccgga aagcggcgca gataatcctt ttcctgcttg ccagtgacgc
     1921 aactcgccgc cgcgcagagt gattaacata ccgtgattat cgggtaaaaa ggccagtgcc
     1981 cagggatggt cgagtttgtc ttgcagtact tcgacattta ccgttgcagg agccgcccag
     2041 agagcggaag aaagacaaat aaggggcaca aggaaaaagg attgtcgatg cataacgcct
     2101 ccggattgac tctggcttaa agcgtagtca gtggaggaga taacaaattc atttttacaa
     2161 aaacttaaac atgaaggggg agacgctttc tcccccttag ttttcaggcc ttctcaagca
     2221 tggcgtgctt ctgcaggctc tggatactca gcgttaagct catcagacaa ttttcaagct
     2281 tatcggcgtt gacggtaata acagtcgggc aatcatggtg cccactcatc aaacatactg
     2341 cggctgtcgc taatgcttct tcagcatgat gaagagcact ccactcttcc tgatccagat
     2401 gaagattcaa ccgcagcgat ttatcgtgca gttcgcgatt cagtttaaaa aagttatctc
     2461 gtagatgatt gctttcgctg acggacatgt atccttttgc cttcctcagt tgcacgtatg
     2521 cagcgaggtc aacgcgtgcg tcgatcaacc ggttcagcag atcgattcgc tgtctgtcaa
     2581 cgaacatact tcgatcctcc tctttccagc cttgttgcga ttaaatagta tggctatttt
     2641 ttgagcaaaa tgctgtccag gatcaattct tcacgccaaa ttaacaaaat ttacatttcc
     2701 ttcgcctccg ccagcgaatg agattgcgat acaaatttta tccagaactc tgtagaatcc
     2761 ctgccctgac ctttcctgta actgaatatt ttttaagcta tgagcaaagt aactccccag
     2821 ccgaaaatcg gctttgtttc ccttggctgt ccgaaaaacc ttgtcgattc agagcgtatt
     2881 ctcaccgaac tacgcactga aggttatgac gtggtaccga gctatgacga tgcggacatg
     2941 gtgatcgtca acacctgcgg ctttattgac agcgcggtac aagaatcact ggaagccatt
     3001 ggtgaagcgt tgaatgaaaa cggcaaggta attgtgaccg gttgtctggg ggcaaaagaa
     3061 gatcagatcc gcgaagtcca cccgaaagtg ctggaaatca ccgggcctca tagctatgag
     3121 caggttctgg agcacgttca tcactacgtg ccaaaaccga aacacaaccc attcctgagc
     3181 ctggtgccag aacaaggtgt gaaactgaca ccgcgtcatt atgcctatct gaaaatttcg
     3241 gaaggctgta atcaccgctg caccttctgc attattccgt ctatgcgcgg cgacctggtg
     3301 agccgtccga ttggcgaagt gttaagtgaa gcgaaacgtc tggtagatgc gggcgttaaa
     3361 gagattctgg tgatctcgca ggatacttcc gcctatggcg ttgatgttaa acatcgtact
     3421 ggcttccaca acggcgagcc ggtaaaaacc agcatggtca gcctgtgcga acagttatcg
     3481 aaactgggga tctggacacg tctgcactac gtttaccctt atccgcatgt ggacgacgtc
     3541 atcccactga tggcagaagg caaaatcctg ccgtatctgg acattccgtt gcagcacgcc
     3601 agcccgcgca ttctcaaact gatgaagcgt ccgggttctg tagatcgcca actggcgcgc
     3661 atcaaacagt ggcgcgaaat ctgcccggaa ctgaccctac gctcaacctt tattgtcggc
     3721 ttccctagcg agacggaaga agatttccag atgctactcg acttcctgaa agaagcgcgt
     3781 ctggatcgcg ttggctgctt taaatacagc ccggttgaag gtgcagacgc caatgccctg
     3841 cctgaccagg ttccggaaga agtgaaagaa gaacgctgga accgtttcat gcagctgcag
     3901 cagcagattt ccgccgagcg cctgcaagag aaagtgggcc gtgaaattct ggtgattatc
     3961 gacgaagtgg acgaagaagg cgcgattggt cgcagcatgg cagatgcacc ggaaatcgac
     4021 ggcgcggttt atctcaatgg tgaaaccaac gttaagccgg gtgatatcct gcgtgtgaaa
     4081 gtcgagcacg ccgatgagta cgatttgtgg ggtagccggg tttaattaat tcaggccggg
     4141 caaaatcatt tgtccgccct gcgaataatg attactgcca gctaatctga gccctgccgt
     4201 ttttcttact cttatacagc cgctcatccg ccagcatttt caagcgttcg acggtggtac
     4261 attcatttga atgcgctaca ccgatactgg tacttaaatt aatgcttccc acctggaaat
     4321 gaacgatttc ttttagaatc gatagcaact cttccagcgt tccacccgta taaatcaccg
     4381 caaattcgtc accgccgaag cggtagattt tacctttatc taccgggatc tgtgatttaa
     4441 ggattttcac cactttcatc aacacttcat cgcccagcaa atgaccgaag gtgtcgttaa
     4501 tgtttttgaa tttatcgacg tcaaacaggc acaaataacc gcctttttcc ggcagcgctt
     4561 ttaaatcttc gtcaaaggat tttctgccca ttgctttggt caggtcgtcc aggaacgccg
     4621 agttatacat actgtagagt tttttgacat cggcagccga aaagaaaagc ccgccatcca
     4681 gtacgcgttc aaaatggtta ttaatgatgg cttctttctc cacaatacca cgattaatca
     4741 ctctgcgtac cagatagagg caactggtac ccgatatcac tgccaccaat gcggcaaaga
     4801 aaataatcaa tgtacagacc cgcaaataaa catctttttt atacgcaaat acgtacaatc
     4861 cggtcggcaa ataggcgaag tgctccatcg cttccgaaac ggtgaagatg gcgtttttag
     4921 cattatattc atcaccggtt taccgttctt aaatagagag aacatcaggt tctcgctgtc
     4981 catcgccagc gattttgaca gtaaagaaaa acgctccaga ccaagcaaaa atatcagact
     5041 acgattgtga gttaccggat agaggtaata gacgaaggtt ttaccgtcaa aagagataaa
     5101 ccctgtattt ttgacatgtc gatacttcac aacctgcata atatctgcac aataattttt
     5161 ctcgaactcc tttacctgaa tacattcgtc actggaaatc agataaggac cgacatcgta
     5221 caaataagag ggggaaaccg agtcggcgta agctgtgcgc aaaccaacca acagctgttc
     5281 ctccgtggga tttagc
//
LOCUS       DAJVRF010000205         5271 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJVRF010000205 DAJVRF010000000
VERSION     DAJVRF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.205"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..461)
                     /locus_tag="OQN13_003762"
     CDS             complement(<1..461)
                     /locus_tag="OQN13_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1616993.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNY"
     gene            complement(461..1381)
                     /locus_tag="OQN13_003763"
     CDS             complement(461..1381)
                     /locus_tag="OQN13_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1616994.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1519..2418
                     /gene="pgrR"
                     /locus_tag="OQN13_003764"
     CDS             1519..2418
                     /gene="pgrR"
                     /locus_tag="OQN13_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCS1616995.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2755..4368
                     /gene="mppA"
                     /locus_tag="OQN13_003765"
     CDS             2755..4368
                     /gene="mppA"
                     /locus_tag="OQN13_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCS1616996.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTARFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(4419..>5271)
                     /gene="ynaI"
                     /locus_tag="OQN13_003766"
     CDS             complement(4419..>5271)
                     /gene="ynaI"
                     /locus_tag="OQN13_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCS1616997.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT         1427 a         1200 c         1222 g         1422 t
ORIGIN      
        1 tcgtaattgc tgaaaccttc ccgaaaaaga cgcccgatat taacacccgt aatactcact
       61 actgctttaa ttcgtttttc tgtcagcgcg gcattaatgg catagccacc acccccacac
      121 actcccagca cgccaatacg ttcagcatta acatatggca gagttactgc ataatcgata
      181 acacggctaa tatcctctac gcgctgtgtc ggatcttcta tccagcgagg ctccccacca
      241 gattctccct ggaaactggc gtcataagcg agaacgagat agcctttttc agccaatgcc
      301 ttgccataga tattgccaga cgtctgctcc ttacaactgc caaaaggatg gacgctgata
      361 atcgccggat actgttttga ctcatcaaaa tttggtggaa actgaatatt ggcagcaata
      421 tgccaggcca tatcactatt gcggatttga atatgtttca ttacagagtt tcctcaaaga
      481 acggagctaa tactgaaatt gcttcagcca cataggcttt gccgtcataa agatccatat
      541 ggtttgcgcc ttcaacaatg tgatagcgtt tatcttggct tgatgctcga tcgtacaggt
      601 cgtcactcat ccatttgctc cctgcctggc tgcccgccac aatctgcatc ggctgagtca
      661 ggtacacttc cgccatatgg taagcatcaa aggtaataat ctggttaagg ctgcgcaaag
      721 tagcgtaacc cggtgctgtt ggatactgcg cgcgaggggt gtggtaatac tcccaggcct
      781 gacgcagttc ttcgttcggc gcatcggact ccttcattgg tgccagtggc ataacggcat
      841 attctccgct gctaatatcg ctggttcggg cgtttgaacc cgcttcaacg tatggcaggg
      901 catcaataga tttcacattg ttttcccaac cattacggaa catcgaacca atattgaccg
      961 cactaacggt accgatggcc ttgatgcggc gatcctgaat tgcagcattg gctgtatatc
     1021 ctgcaccggc acaaattccc atcgcaccaa ttcgggtatt gtcgacataa gaaagcgttg
     1081 tcaggtaatc aatcacggca ctgatgtctt cagtacgaat gtatgggttt tctaactgac
     1141 gcggctcgcc gccactttca ccttgataag atgcgtcata agcaatagtg acaaatccct
     1201 tttccgccag ttttttggca taggttccgg ccgtttgttc tttaacgccc ccacctgggt
     1261 gagataccac aattgcctga tactgacggg tttcatcaaa ttttggaggg aaatagatca
     1321 ctgcagacaa agagatggtt ggattattgc tgttagtgaa gctgacttta ttattcatca
     1381 ttccgttcct catggagttg tcggttcgtt ttaacggttg gtgatatcac tatagatatt
     1441 gatcattaag ttgattagac ccaaaattat gattagacct atcatttaaa tgattaatag
     1501 attgcatgga gatactgaat gaagcgtgaa gaaatcgctg atctgatggc gtttgtcgtc
     1561 gttgcagagg agcgtagctt cactcgtgca gcagcccgcc tgagcatggc gcagtcagct
     1621 ttaagccaga tagtgcgtcg tatagaagaa cgattgggat tgcggcttct gacgcgaacc
     1681 acgcgcagcg ttgttccaac tgaagcgggc gagcatcttt tgtctgttct tggccctatg
     1741 ttgcatgaca tagattcagc cctggcatcc ctgagcgagc tgcagaaccg cccatccggg
     1801 acaatacgta ttactactgt agaacatgca gccaaaacga tattgttacc agcaatgcgc
     1861 acattcctga aatcgcatcc tgaaattgat attcagctca ccattgatta tggtttgacc
     1921 gatgtcgttt ctgaacgttt tgatgcaggc gtccgtctgg gtggggagat ggataaagat
     1981 atgatcgcca ttcgaatcgg gccagatata ccaatggcta ttgttggctc accggattat
     2041 ttttctcgcc gaagtgttcc aacgtcagtg tcacaattaa tagatcatca ggcaattaat
     2101 ttgtatcttc ccacatcggg tacagcaaat cgctggagat taatacgcgg tggacgtgaa
     2161 gttcgtgttc gcatggaagg tcagctttta ctgaatacga tagacctgat cattgatgct
     2221 gcaattgatg ggcatggatt ggcgtatcta ccttatgatc aggttgagcg ggctattaaa
     2281 gaaaaaaaac tgatacgtgt tttggataaa ttcacaccag atttacccgg ttatcacctg
     2341 tactatccac accgtcgaca tgctggctcg gcattctcat tatttataga taggctgaag
     2401 tataaaggtg ctgtttagca ctacttgctg atacattaat ttaatttttc tcttaacgta
     2461 ttctcagttc ctttcaacgt tttggttatt ttttattctt cgtacaatgg cgacagatgc
     2521 tgattatgat accgaaaact ggtttgaacg tgcgaagcct gaacgtagtg ttcggtgttc
     2581 tatgtgcttt accgcatttt gtcgtctatt atttacacta aatctgattt aatatattga
     2641 tacataaaac attttatgct ccctgtgtca cttttttgat ggaagttgtt tgcatttctt
     2701 taaggcgaaa caaataatta cgcatcaatt ttaatgtcgg ttagagggaa acttatgaag
     2761 cactctgttt cagtcacgtg ttgtgcgctg ttggtcagca gcatttctct ttcgtatgct
     2821 gcagaagttc cgagcggcac agtactggca gagaagcagg agctggtgcg ccacattaaa
     2881 gatgagcctg cgtcgctgga tcccgctaaa gccgtgggcc tgccagagat tcaggtcatt
     2941 cgcgatctgt ttgaaggact ggtgaatcag aacgagaaag gggagattgt ccccggcgtt
     3001 gcgactcagt ggaaaagtaa tgacaaccgt atctggactt ttaccctgcg cgataacgca
     3061 aaatgggcgg atggcacacc ggtaacggcg caagattttg tctacagctg gcagcgtctg
     3121 gtggacccga aaacgctatc gccatttgca tggtttgccg cgctggcggg aatcaacaac
     3181 gcacaggcga ttattgatgg taaagctacg ccagaccagc ttggcgtcac cgcagttgat
     3241 gcccatactt tgaaaattca gcttgataaa ccgttgccgt ggtttgtgaa tttaaccgct
     3301 aactttgcct tcttcccggt gcaaaaagcc aacgtagaaa gcggtaaaga gtggacgaaa
     3361 cccggaaatc tgatcggcaa tggcgcttat gttcttaaag atcgcgtagt caatgaaaaa
     3421 ctggtcgtgg taccgaatac ccattattgg gataacgcca aaacggtact gcaaaaagtg
     3481 accttcctgc caattaatca ggaatccgca gccactaagc gttacctcgc gggggatatt
     3541 gatatcaccg aatccttccc gaaaaatatg tatcagaagc tgttgaagga tattccgggg
     3601 caggtttata cgccgccgca gctcgggacc tattattatg cgtttaacac gcaaaaaggg
     3661 ccgacggcag atcagcgcgt tcgtctggca ttaagtatga cgatagatcg ccgcctgatg
     3721 accgaaaaag tattagggac gggcgaaaag ccagcgtggc attttacacc agatgttacc
     3781 gccagattta cgccggaacc ttcgccgttt gaacaaatga gtcaggaaga actgaatgcg
     3841 caggcaaaaa ctttgttgag cgcagctggt tatggtccgc aaaaaccgct gaagctgacg
     3901 cttttgtata acacttcaga aaaccatcaa aaaattgcga ttgctgtagc atcgatgtgg
     3961 aaaaagaacc ttggcgtaga tgttaaattg caaaatcagg aatggaaaac ctatatcgat
     4021 agccgtaaca ccggcaattt tgatgttatc cgcgcctcat gggtggggga ttataatgaa
     4081 ccctccactt tcctgacatt attaacgtca acgcattcag gaaatatttc acgctttaac
     4141 aatccggcat acgacaaagt tctggcccag gcatcgacgg aaaataccgt taaagcgcgt
     4201 aatgccgatt acaacgcggc agaaaaaatc ctcatggagc aagcaccgat tgcaccaatt
     4261 tatcaatata ccaatggacg attaatcaag ccgtggctga aaggttatcc cattaataac
     4321 ccggaagatg tggcgtacag ccggactatg tatattgtga agcattgatg tgatgggaac
     4381 tggcgttacc cttgtggata acgccagtga tgtctgattt agcgcccctg ttccggcggt
     4441 gtaatgttat ccatatacag cgtctggctg gggaaggcaa agtccgcgcc gtgtgactgc
     4501 acaatatcga taatcttcaa ataaacgtct tgctgtgcag caagccattc agcccatacc
     4561 gtggttttgg taaagcaata aaccataata ttcaatgacg agtcggcaaa ctggttgaaa
     4621 taaaccagta aggtttgtcg ctggtcgatg gccgggtgat ttttcagcat ctcacgtacg
     4681 gcttcgacaa taacgcccac ttttgccgca tcctcataac gtaaaccaat ggtcgtggta
     4741 atgcggcggt tggtcattcg tcctgggttt tctacgctga tcgacgaaaa cagcgagttc
     4801 ggtacgtaca atggacgatt atcaaaggtc gtaattttgg taattcgcca gccaatttcc
     4861 gctactgtac cttcgatatt tctgtccggt gaacggatcc agtcgccaat actgaaagga
     4921 cggtcgaaat agagcatgat cccggaaaag aaattactca gaatatcttt accggccata
     4981 ccgacagcca gaccaccaat accaccaaag gtcagcaagc cagaaaggct catgccgaaa
     5041 tgttcgccat aaagcagaac aagcaccaca ataatggtga ttttgataat gcgcgacata
     5101 atccgcgcac tggtgatatc gcgacctttt ttaatctgct gtttttcaaa ctgattaatc
     5161 agcaaaaata gcttaatcgt cagaataacc gcaatcaggg acgtacagat aaaatcgata
     5221 acgtctgggg tgataaattt gagtttatag ttttctataa cataattaat a
//
LOCUS       DAJVRF010000206         5152 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJVRF010000206 DAJVRF010000000
VERSION     DAJVRF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.206"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..218)
                     /locus_tag="OQN13_003767"
     CDS             complement(<1..218)
                     /locus_tag="OQN13_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCS1616998.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKA"
     gene            complement(218..433)
                     /locus_tag="OQN13_003768"
     CDS             complement(218..433)
                     /locus_tag="OQN13_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCS1616999.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            complement(1114..1188)
                     /locus_tag="OQN13_003769"
     tRNA            complement(1114..1188)
                     /locus_tag="OQN13_003769"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1153..1155),aa:Gly,seq:tcc)
     gene            complement(1192..1267)
                     /locus_tag="OQN13_003770"
     tRNA            complement(1192..1267)
                     /locus_tag="OQN13_003770"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1232..1234),aa:Thr,seq:tgt)
     gene            complement(1270..1346)
                     /locus_tag="OQN13_003771"
     tRNA            complement(1270..1346)
                     /locus_tag="OQN13_003771"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1310..1312),aa:Arg,seq:tct)
     gene            complement(1348..1424)
                     /locus_tag="OQN13_003772"
     tRNA            complement(1348..1424)
                     /locus_tag="OQN13_003772"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1388..1390),aa:Ile,seq:cat)
     gene            complement(1614..2177)
                     /locus_tag="OQN13_003773"
     CDS             complement(1614..2177)
                     /locus_tag="OQN13_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCS1617000.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(2281..2499)
                     /locus_tag="OQN13_003774"
     CDS             complement(2281..2499)
                     /locus_tag="OQN13_003774"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617001.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(2658..3044)
                     /locus_tag="OQN13_003775"
     CDS             complement(2658..3044)
                     /locus_tag="OQN13_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS1617002.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(3034..3405)
                     /locus_tag="OQN13_003776"
     CDS             complement(3034..3405)
                     /locus_tag="OQN13_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCS1617003.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(3405..3662)
                     /locus_tag="OQN13_003777"
     CDS             complement(3405..3662)
                     /locus_tag="OQN13_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617004.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(3659..5050)
                     /locus_tag="OQN13_003778"
     CDS             complement(3659..5050)
                     /locus_tag="OQN13_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCS1617005.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            complement(5047..>5152)
                     /locus_tag="OQN13_003779"
     CDS             complement(5047..>5152)
                     /locus_tag="OQN13_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1617006.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT         1271 a         1414 c         1140 g         1327 t
ORIGIN      
        1 gctttgcatt ctgcttcggt atacgtttta ccgagcatga tgtcttttcc ggtgtgtccg
       61 tgacatacag tccatacacc aatgatatct ttgtatggta tgtagctgac accttccaga
      121 ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaacagc cccaccacca
      181 atagcagctg caacagcctt gcgtaatgat ggcgacatta ttcacctctc gcagccttac
      241 gcttgtcttc tcttattttg aaatacagat ttgtcagata agtcagaagc cccagaagca
      301 gacttcccag cacaccaatc gcagcccact gtgatggact gacctgatcc aaccactgca
      361 aaaaccagta gccggcactg ccggcggagg tgccgtaggc aatgcctgtt gatatttttt
      421 ccatctgata catatcccgc cccccgacag acctgtgcta tcggaaagaa aaaaggccat
      481 cagcaaaact ctgatggcct gaatcacctt taccaatatt gtatgaaaaa acacgcacga
      541 ctaattgaca ataattttca tttccattaa aaaagatcgc gtagcattct taattcatga
      601 ggaactttac ccgccagcaa tctgagtagc gaaagctgtt catccccaat gtttttgctg
      661 gcgggtcctt ttttctacgg tcctcctccc cggagggggg catttcatta tttttctaat
      721 cgttactggt gagaaggcgg aagaaaaagc cagttcttcg cccctaaata acagcatgat
      781 tccagtccat gcaaaatctg atttccagct cacacatacc gagcgctgta caaaaaaccg
      841 ccagccaagg cagttaagag tgtgttcccg gggtttgctt agaatatttt gtaagttgtc
      901 cgaagagata tttacaacac cagaatgatg aatcatcagt cccctgccag aggttcacta
      961 cgtaacctga ccgacaatgc accattctgg tgctgaaaaa agagcactgg aactgcaaca
     1021 aaacaacatc acagaacagg agatggtaag gagtcagaca ttcccacaca atattgtgtc
     1081 agtgcaaata acaaccttcg tctcaaatct cgctggagcg ggcagcggga atcgaacccg
     1141 catcatcagc ttggaaggct gaggtaatag ccattatacg atgcccgcat atggtgccga
     1201 ctaccggaat cgaactggtg acctactgat tacaagtcag ttgctctgcc tgctgagcta
     1261 agtcggcgct ggcccaccac cgaggactcg aacctcgcac cgtcaactta gaaggttgat
     1321 gctctatccg gatgagctag tggtggttgg tggcccttgc tggacttgaa ccagcgacct
     1381 ggcgattatg agtcgctcgc tctgaccaac tgagctaaag ggccggaggc agaataataa
     1441 ccatatgtca tcacatctgc aaactcatct gaccaccagc acgtttaacg tcctgtgccg
     1501 tttttcaggt ataaaaaaac ccgctcatcg gcgggtttaa gctgtgtggc gtagtaacca
     1561 ctcttaacag gatattcaac tttttacgat cgtaaagttt tcggaaaaaa tttttaaaac
     1621 cgcatcaacc gctccaccag ttgctcttta cgggcaacga tccacccgtg ttgctccaga
     1681 taaaatttaa accgttccag agtacatacc atcgcatcgg cgggtacctt ttccgtgaac
     1741 tcaacctgac cgtgtttatc gaagtggctc agtaatgcgc atccatcatt ttcgatagat
     1801 gtgttttgtg ccgctggtgg ctgtttctgg ttgaaataac agtcttccag tttttcgaac
     1861 acttcccacg cctgatcggt ttcgagcatt ttggcgtgac gggctgcgcc acgttctgtc
     1921 cagaggatga gggagcgaac gttacgggca attttcacag aaactcttaa agagttttcg
     1981 ttcttcaact ctcgtaacgc atcaccttct atcaggaaaa aatgtttacc ttctataaac
     2041 cgttctttgt ttcgattgaa attgtttgtc aaacgctggc gctctgttcc gtacaaatca
     2101 gccagcagct ctgttgtcat gacaggaaga tggttatgcg taagtgacgg gagtttttca
     2161 atagacgtgc gattcataga catgtccttt cgaatatttt gatttacccc ttttgagagg
     2221 gtgaccgggc gctcaaaacc gtcgaaagtc ggcgggcgta tttccattgc tggtattgta
     2281 ttagccgcac acccggtcat aaaccaagaa ttctggacac aaaaaaacca catggctatc
     2341 gggtgtggat accgctttcg aggtgttttg agcaccgcgg ggaaatgtaa actgcaaacc
     2401 tgaaactgtc aacaccgtca ggttccaatc atcgggtggt gagacgcaca gggttggaac
     2461 taccgggaaa ccgaccggcg agcttttcag ctcccccatg cgccccacca taattcagat
     2521 gtgcgcgtgc atacgacaat aaaaaacacg ctcgcggcgt gtgtctgtcg cggtctctat
     2581 ccggggttcc aatcccgacg ccagattttg ctggcgcgtg aggaatatag ccccggacaa
     2641 tgtgtctggt caagcaccta cataattcgt tctacgtatc tgtccatctc cagccggata
     2701 tcaagcatca tcaacatgcc atcaataacc ccttccgctt tctgcaggcg cttgccaata
     2761 caggtatccg aacacccatg tttccgtgcc agactcataa aagtcattcc acctacgtaa
     2821 taatccacca gcaaatcgtg caaatcctga tttttcttgt tcaaccgggc catacagcca
     2881 caaattatca ttgcatcatc atcagaacac tgagggcgtg atttcacttt cgacggaatt
     2941 aaccctttaa aaccagcagc gatcgacgcc caggatacat cttcatgagt gtttgcagcc
     3001 caagcccccc accgttccat aacctgctga atatcacgcg ccatcgttat cacctgtgat
     3061 ttcataaatc ttcacgccca gtcgcccacc aggaacgagc tgaccgcgca caatattgat
     3121 ttcatcaaac tgctcgtcgt ctatgagtag tccggcatgc gtcagcgcat ccagtggtgc
     3181 tttcaggata ttgtccaggt cacgacggcg cttatccggt ggctctgcaa taatctttat
     3241 cgccagcctt ccggacaggt ttaatttcag ccgctgctgg cgaacaatta gcgtcacatc
     3301 acggcgataa cgctcaccaa cttttgatac aaaatatgtg ctgccacgac gtcgccagta
     3361 agtattcacc gtcggcggat aaggtaaaat aaactcatgg cgcatcagcg cagcacctcc
     3421 tgcaccattt tttcaaactt tccaactttg gtttccagct ctgccacaca atcaacgagc
     3481 tcatccaccg ctttttgtgc gcgatgtttc gcctgcatca gttccctgag cgctggcacc
     3541 atatcccgac gaatggcatc ttttgttaca cctgtttttt ccagttgttc cgcctgtcgc
     3601 aacatttcct gcgcgtgttt acgcaattgt tcagaggtaa aagtcattgt ctggttgttc
     3661 aaaagaaacg ctccatctta ctgctgtcgg tacgtgtatt gctgtatctg cgcggctggg
     3721 gctgccgcat tggggtggaa agaacctgtg cgctttcctg gtccacaggc agaaaatgtc
     3781 cgttataaaa acgccggtaa atcgtcccca gagaaccgtt acgttgtttc gtgatattaa
     3841 tttctgctat gcctgtagcc tgtgtatccg ggttgtacac ttcatccctg taaagcatca
     3901 gaatgatgtc tgcatccgcc tctatttctc cggaattttt caggtctgag ttcatgggac
     3961 gtttattggg tctggactcc acaccgcggg agagctggct cagcgcaatc aacggaaaac
     4021 caccggattt tgccaggcct ttaagcccct ttgagatttc acccacggca aggtcatgac
     4081 gccccgtggt tcgggttttt atcagcccga gataatcaac caccaccagc gccgtttccg
     4141 gatgtttaat cagatggtgt ttcgttgttg cgcatatctc atcaatggtc aggttcgcct
     4201 ggtccaccat ccagatattg cgcccggtca tccgccccac cccttgtgag aaacgcgccc
     4261 agtcttcatc ttcaaagtga gccacagatt tcaggcgtga tactggcatc cctccagccg
     4321 cagacaccat acgttcacca atctggatgt tcgccatctc catggtgaac agaagcacac
     4381 catgcccctg ctcagtcacc ttgtcgatga tgtccagcgc aagttcggtt ttccccatcg
     4441 aaggacgggc ggcaatgaat accaggtctc cgggctccat accgcctgtt tttgcgtcca
     4501 gttcatcaat accggtcatc aacgtcctgg atttctccag cccctgattc cggcattcaa
     4561 cacgctcaac cacttccgga agcacatcat caatatgtac cggctgaatg acgccctttc
     4621 ctgtcgacag tgtgaccatc atgttctgcg catccttcag ggcatcttca gctgcttcac
     4681 aggtatgcgc atcacgtaat ttctgcagcg cctcattcag tgttttttct gcatcgcgca
     4741 gtgcggcatt gcgccgcaac gctgcaacat agtgctccag tgaagacttc acccaggttt
     4801 tacgcccggt atcagtaatc accggggcaa gttccggcat ctcattacac aacagcacgg
     4861 ggtcaatcac tcctgaaaca cgggcctgtc tgcagatgcc tgtgtagata tcccgatacg
     4921 ctcgtacaga aaaaatgtcc gccggtagtg tgatcagaat atccatcact tcatgatctg
     4981 ccccacgcag aaagaatgcg ccaatgacag cgccttccag gtcatcatta cgccagactg
     5041 gtgttgtcat gcagccacac ctctgataca agaacggtag ctgggccagt tgaacgacaa
     5101 ccagttgcgc cccccgtctg tgatcctgtc ggcaatgcgg gggctgatga ac
//
LOCUS       DAJVRF010000207         5114 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJVRF010000207 DAJVRF010000000
VERSION     DAJVRF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.207"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..379
                     /locus_tag="OQN13_003780"
     CDS             <1..379
                     /locus_tag="OQN13_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617007.1"
                     /translation="KKTNIYFDDNFLNIYKRSQDRAPQIISDLKKHNIEIGEKLNDCR
                     KKLNISDVKLTYYWFVTDTGGVDYLDGVLTYNFQGKNRAENFHKQLLQYAYINYSKNQ
                     IPRDFVTSIKNSIYYKIEDCTKF"
     gene            complement(575..2125)
                     /locus_tag="OQN13_003781"
     CDS             complement(575..2125)
                     /locus_tag="OQN13_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCS1617008.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKK
                     ASIPQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGINRTPEVHLGP
                     VQAASLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPEL
                     LLQYRKVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARY
                     LDKLGRNVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHER
                     NEFRNKLQQAESEWDKENAAFKRTEGYKYVGELDRYREREILAAASLENTRQKVAEEV
                     SVARSQMLSLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALCIQRSWARGVSRSN
                     ERDQDMER"
     gene            2297..2743
                     /locus_tag="OQN13_003782"
     CDS             2297..2743
                     /locus_tag="OQN13_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HCS1617009.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFSAAELVGALAGIAELPHNHPKWQEWEKRGKELLTKDSA"
     gene            complement(2937..3872)
                     /locus_tag="OQN13_003783"
     CDS             complement(2937..3872)
                     /locus_tag="OQN13_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCS1617010.1"
                     /translation="MSAALQYFEENLPHRPYHTDELAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPENGHAHLLYALNLAVRTAPDASV
                     KALKYAAAIERALCEKLDADVNYSGLICKNPFHLEWQVMEWREEAYALDELADYLDLR
                     ASERRSVDKHYGLGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTPESFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLNVHRNTIRNWAMHK"
     gene            4384..>5114
                     /locus_tag="OQN13_003784"
     CDS             4384..>5114
                     /locus_tag="OQN13_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617011.1"
                     /translation="MGKMNINFKHILPLLSSFFISGCDSDGISKVKSFVYYDIDDSMT
                     VGKAFDTRSDCINGTWSEKEDDRGRTIVTYTCDISESGLKIINSAIMQEPEDKAEYSI
                     AQNNNSIKSTNESLEEYKQKTPLVEKTKEVIIDHIRKLNSAFNEDPMIYSKLTISPYL
                     DRMLYLKDHNDNALNEICGGYEYIQSADSYSWRDVSEEYAKESCEKHIYKVYQSFKKN
                     ASPLITKNFPGFYERVPPCENADEC"
BASE COUNT         1417 a         1244 c         1127 g         1326 t
ORIGIN      
        1 aaaaaaaaca aatatatact ttgatgataa ctttctaaat atctacaaaa gaagccaaga
       61 tagagcacct caaataattt ctgatttaaa aaaacacaat atagaaatag gtgaaaagtt
      121 aaatgattgt aggaaaaaac taaatatctc agatgtcaaa ttaacctatt actggtttgt
      181 aacagatacc ggaggagttg attatttaga tggggtgttg acatataatt ttcagggaaa
      241 gaatagagct gaaaactttc ataaacaact attgcaatat gcatacatta actacagcaa
      301 aaatcaaata cctcgggact ttgtaacatc aataaaaaat agtatttatt ataaaataga
      361 agattgcact aagttttagt ccttccctta caggtggtta cacatataac cacctgcaaa
      421 tatttaacac ctgttaactt cattaatctg aactgtgagt aaatcaaaac gacaattttt
      481 ctcattcaga aaacgtttca cgggacgcgg aattttttcg cgttccgtga aacgttggcg
      541 gggttcgggg cgcagtcccg atattgctac attttcaacg ctccatatct tgatcacgtt
      601 cgttactacg tgataccccc ctggcccagg atcgctgtat acacagtgct ttttcctcct
      661 gttgctgccg ttcctgcgcc atttctgcca gtaactcatg tcgcatttga gctttttcat
      721 ctccagatat actgagttca ggctccagag ataacatctg gctacgggca acggatacct
      781 cttcagcaac cttttgcctg gtgttctcta aagatgccgc cgccagaatc tcgcgttctc
      841 tgtacctgtc cagctcccca acgtacttat atccctcggt acgcttaaaa gcagcgtttt
      901 ctttgtccca ctcggattcg gcttgctgta gcttgttcct gaattcattc ctttcatgtt
      961 cagcggtttc cagcacactt ttttgctcca gaatacgcgc atcaatctcc cttttgtcag
     1021 ccccccgaaa caggccacga atcccattca aagcattgcg ttgttcctgt agctcccgca
     1081 gctccctttg tgcggaaaca acattacgcc ccagtttgtc taaataccgt gccccttcat
     1141 atattcgtct actccattcg gaaagagagt cttttttagc tttcgcattc tgaagcagct
     1201 tatgttcttt gagcgcattg ggattggcat cccctaaacg agcaagcctt gcctctccct
     1261 gaatgaccgt tttcataacc ttgcggtact gaagcaataa ctccggtgaa cgggctgaac
     1321 gaacaggctc aactgccttg attttttgct tttgctcctg ttcctgctga atggcatttg
     1381 cggcatctct ggcggtcttg agttcccgct cggcattcct ccgttcctgg attgcaacaa
     1441 tctgctcccc attcagactg gcggcctgaa cggggccaag atgaacttcc ggcgtgcgat
     1501 tgataccctg ttctttcaga gaacgatgat ctacgcgcga ctcgtgaccg tgtcgcgcaa
     1561 ggtgctcatt ctgcacatct gcccagcggg aacgcagcgc aacaagggcg gcttttcgct
     1621 ctccggctgt ctggggaata ctcgcttttt ttgcgcctcc ccgctctggt gatttgctgt
     1681 tggcacgttt aaaaaattgc tgtggatcgc gttcaatacc atcattaatc cgctcgcaaa
     1741 acatgatatg cgcgtgtggc tgttcctttc cgtcgattgc acctggcgga ttgtggatcg
     1801 cgtactggta ggcatggcga tcgccaagtt ccttttgcac gaaatcttca acaagttcta
     1861 tccgttgttc gcgtgtcagt tcacgcggta acgcgatctc aatttcacgg tatgcagtcc
     1921 cgttttttcg ttcaaattca tctgcggctt tccatagctc tgcgggattt ttcttcgccc
     1981 atgatggcat gttaccggat gacgaaaatt ccagatcctc ccgcgatttg taactcttat
     2041 attcgccgga tctggaaatg taatcagcat gttttaaggc actgcctttt gctccagctt
     2101 tgacagtgca atggtagctt gccatctgcc ttttagccct ccgcaggagc gcacagtttc
     2161 tcgacgaagg agagaaacta ctaagtgcgc atccctgaat taatcatgga tgattttacg
     2221 gtctcacacg caggcaaaca aggtgatatt atgcataata acaactcaca ttaacggcaa
     2281 aagtgcagag gtaagaatga caaatcgtga agagtggtta agtgcaaaaa ttgcctacat
     2341 caacggactg aaatcaccat cagagcagca gagattactt gtgttactgg cagagaaaaa
     2401 aaaccgaaca acgacggatg aaaaaacgtt gtccgcactg atacgggcag aaaaaaccgc
     2461 agagaaagca gccgcagcaa aagccagagt aacggcaatc atagcggcag aacggaaggc
     2521 agccgcaagg gcggaacgaa aggcgcggga tcatgaactc tacaaggccg caggcctcat
     2581 gattgtggca ggactggtgg acagcaaaac cggaaagcca aagtttagtg ctgccgaact
     2641 ggtaggcgca ctcgcaggaa tcgcagagct accgcacaac catccaaaat ggcaggaatg
     2701 ggaaaaacgc gggaaagagc tactcaccaa agacagtgcg taagagcctc cgttcacgtt
     2761 ccagttccct ggtgcttcct cgctcattcg ctcgctacgc tcggtcatga gactgcggcg
     2821 agcacgacca cctcaatcat acggtaagcc gcaccattgc tgggctgaaa ggagaaaacc
     2881 gccggaaagc ccgctgctca aaaagacgcc gaaaaggcgc tgttatctga tataggctat
     2941 ttgtgcattg cccaattacg gatcgtatta cgatgtacat tcagcatcgc cgcaatagct
     3001 ttctgagaat atccattctc cagcatacat agcgccatga aacgcttgtc atcgtaggct
     3061 tcgcctttag cgatgccacc tttccggcct ctcttggcct gtatttctgg tgtgtgcgta
     3121 tcagccacat actgtgcgaa actttccggc gtgaagttcc tgtgcgtgta tttcgcaatg
     3181 ctcttgccaa tagcccgaca ttcaggcgga gaaagcggaa cgggaagcga tgcgttgtac
     3241 atttcgacac gctgaatcac agcatcaagc cactgtgaga atgctggcca gccctgccga
     3301 atcgccctgt aagcccattt gcgcgtcatt tcgaacagat ggcagtttcg ccccagcccg
     3361 taatgtttat cgacgctacg acgctctgag gctctcaaat cgagataatc agccagttca
     3421 tcgagggcgt aggcttcctc gcgccactcc atcacctgcc attccaggtg gaacggattt
     3481 ttgcaaatca gtccgctgta gttcacatct gcgtcaagtt tctcacacag cgcacgttca
     3541 attgcagcgg cgtatttcag cgccttaacc gacgcatcag gcgcggttct cacggcaaga
     3601 ttgagcgcgt agagcaggtg agcgtgcccg ttctcgggat tttttacggt gatgttcggc
     3661 gcgggggcgt tccggtcgct ccagtcaatc gcagctccta cgcggtcaac gtcaaaaacc
     3721 agccagaact gcgcatgagg ctgattttgc tggatgtagc gagcaagcag cgcacgccct
     3781 ttgccggaga tacgaagacc gaaagcgagc tcatccgtgt gatagggacg gtgaggtaaa
     3841 ttttcttcga agtattgaag cgcggcgctc atgtgttacg attccttcca ggttatcgac
     3901 cgacatcgcc cctgtatctt cgtgaaagtc ttgccggata gacacgaatt tcaggggcgt
     3961 ttgtttttca gtcgcttaca ctacccccaa cgacaaaacc tgtcgagtct tcccgcaaac
     4021 ccgatccatt taccataact cccgttgtgc gcccccctta aaacgccctc agtcgctttc
     4081 tgtcgcaaaa aacgcaaaaa gacagggaaa caacaggcca gaccaaaaaa acgcgccaga
     4141 attgttttca gggggctgca taactatgca cgaaagcgat tcatgtccgg tttcactcgt
     4201 tttgaacagc gaacaggtca aaacagcaaa ctttctcatc cagaaaaagg tgaactggtg
     4261 cgaaatcttt tcgaactgcg taagacgttg agcacaaaca tagtaagtcg gtttatcggt
     4321 atgtatggat tccattaaca tatcgcctgt attaatattt cctcgttatt gttagaaata
     4381 aaaatgggaa aaatgaatat taactttaaa catatccttc ctttattgtc gtcatttttt
     4441 atttcaggat gcgactctga tggaataagc aaggtaaagt ctttcgtata ttacgacatt
     4501 gacgactcaa tgactgttgg taaagccttc gatacaagga gtgattgcat caatggcaca
     4561 tggagtgaaa aagaggatga tcgaggaaga accatcgtca catatacctg tgatattagt
     4621 gaaagtggcc taaaaataat aaattcagcc attatgcaag aacctgaaga taaggctgaa
     4681 tattctatag cacaaaataa caacagcata aaatcaacca atgaatcact agaggaatac
     4741 aaacagaaaa cgcctctggt ggaaaaaaca aaagaggtca taatagatca tataagaaag
     4801 ctaaacagtg cttttaatga agatccaatg atatatagca aactcacaat tagcccttat
     4861 ttagacagaa tgctatatct aaaggaccat aatgacaatg cgctaaatga aatttgtggt
     4921 gggtacgaat acattcaatc tgccgatagt tacagttggc gtgatgtaag tgaagagtat
     4981 gcaaaagaat catgcgaaaa gcatatatat aaagtatatc aatcgtttaa gaaaaatgca
     5041 tcaccattaa taactaaaaa cttccccggt ttctacgaac gtgtcccccc ctgtgagaac
     5101 gcagatgaat gcat
//
LOCUS       DAJVRF010000208         5044 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJVRF010000208 DAJVRF010000000
VERSION     DAJVRF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.208"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            73..1601
                     /gene="lsrA"
                     /locus_tag="OQN13_003785"
                     /pseudo
     CDS             73..1601
                     /gene="lsrA"
                     /locus_tag="OQN13_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            1595..1903
                     /locus_tag="OQN13_003786"
     CDS             1595..1903
                     /locus_tag="OQN13_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1617012.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLMRKVVSAMISWIILSRARQKYGAASVVVDEQSWLSSGVKKSPY
                     "
     gene            complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="OQN13_003787"
     CDS             complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="OQN13_003787"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCS1617013.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3750..4700)
                     /locus_tag="OQN13_003788"
     CDS             complement(3750..4700)
                     /locus_tag="OQN13_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCS1617014.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4839..>5044)
                     /locus_tag="OQN13_003789"
     CDS             complement(4839..>5044)
                     /locus_tag="OQN13_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HCS1617015.1"
                     /translation="ALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSL
                     SEEQQRYIVAVIYHWLVVQMNQP"
BASE COUNT         1316 a         1288 c         1230 g         1210 t
ORIGIN      
        1 taaggttatg aacaaattaa aagcagaaat acatttgttc aaaactcacc tgcaaaactg
       61 aacgggggaa atatgcaaac gagtgatacc cgcgcgttac cgctactttg cgcccgctcg
      121 gtttataaac agtattcagg ggtcaatgtc ctgtcgattt tacgttgcat caaggggagg
      181 tccacgccct gctcggcggc aatggtgccg gtaaatcgac gttaatgaag attattgccg
      241 gtattacccc tgctgatagc ggtacgctgg agattggggg caacaactac gtcagattaa
      301 cgccagttca tgctcatcag ctgggtattt atctcgttcc ccaggaaccg ctgcttttcc
      361 caagcctgtc gataaaagaa aacatcctgt ttgggctggc aaaaaaacag ctctccatgc
      421 agaaaatgaa gaacttgctg gcggcgctgg gctgctagtt tgatctgcat agtctggcag
      481 gatcgctgga tgtcgccgat cgccaaatgg tggaaatcct ccgcggcctg atgcgcgact
      541 cgcggattct gatcctcgat gaacctaccg cctcgcttac ccctgcggaa accgaacgct
      601 tgtttcgtcg cttgcaagag ctgcttgcta ctggcgtggg tattgttttt atctcgcata
      661 agctgccgga aattcgccag attgccgatc gaattagcgt gatgcgcgac ggaaccatcg
      721 ccttaagcgg caaaaccagc gaactgtcta ccgatgacat tattcaggcc atcacgccag
      781 tggtacggga aaaatcgctc tctgccagcc aaaaattatg gctggagtta cctggcaacc
      841 gcccacaaca tgccgccgga acgtcggtgc tgacactaga aaatctgacc ggcgaaggtt
      901 tcaggaatgt cagcctgacg ctcaatgccg gagaaattct gggtctggcc gggctggtgg
      961 gggccggacg cacagaactg gccgagacgc tctatggtct gcgtactttg cgtggcggac
     1021 gcattatgct gaatggtaaa gagatcaata aattatccac tggagaacgt ttactgcgcg
     1081 gtctggttta tctgccggaa gatcgccagt catccggact gaatctcgat gcttcgctgg
     1141 cctggaacgt ctgcgccctt actcataacc ttcgtggatt ctgggcgaaa accgcgaaag
     1201 ataatgccac cctggaacgt tatcgtcggg cgctgaatat taaatttaac caaccggaac
     1261 aagctgcacg gacattatcc ggtggcaacc agcaaaaaat cctgattgcc aaatgcctgg
     1321 aagcttcgcc gcaagcattg attgtcgatg agccgacgcg cggcgtggat gtctcggccc
     1381 gtaatgatat ctaccagctg ttgcgcagca tcgccgcaca gaatgtggca gtgctgctta
     1441 tctcctctga tctggaagag atcgaactga tggcagatcg tgtgtatgtg atgcatcagg
     1501 gcgaaattac ccactctgca ctgaccgggc gcgatattaa tgtcgagact attatgcgcg
     1561 ttgccttcgg cgatagtcag cgtcaggagg cgtcatgctg aagtttattc agaacaaccg
     1621 tgaaatcacg gcactgctgg cggtggtgct gctgtttgta ttacccggtt ttctcgaccg
     1681 ccagtattta agtgtgcaaa cgctgaccat ggtttatagc agcgcgcaaa tcctgatcct
     1741 gctggcaatg ggcgcgacgc tggtaatgct tatgcgcaaa gtagtaagcg cgatgatatc
     1801 ttggataata ctgagccgtg ccagacaaaa atacggcgca gccagtgttg ttgtggacga
     1861 acaatcatgg ttatcttcag gcgtgaaaaa gtcgccctat taaacgtgat tacgatcaca
     1921 ttctcaagac gctacttaca aattacctac aagcttacaa cattaacagg cgctgcatgt
     1981 ggcatctgtt aatgataaag gcacatagga tgttgtaact aatatagtga aataagaaac
     2041 ccggtcaaaa ccgggttcag aagtaacggt gttattagca caacggacgt acagcttcgc
     2101 gcatcccttt ttcaagaatc gcatccaggt cattagcaac ctgctcgacc agaccaggca
     2161 cttgcgtcag gtcctgctcc cagtggtctt tttctgccaa cacaatcgct accagttcct
     2221 gggtaccgat aacatggtca cgatgctggc tccagagttg ctgataacgt tccagccagt
     2281 gtgcatcgtc ctgtaccgga tatgtttctc cgttacgctc accacgatag aacgcaatca
     2341 atgccgctaa tgcgaaagta aggcgcgccg gaagtgtgcc gtttgccttc tgccctgcca
     2401 gcagctgcgg caggatgcgg gtgcggaact tggtcatacc gttgagcgcg atagacagca
     2461 gctgatgctt atacgggtta cggaaacgcc cggtgactgc actggcgaaa gattccagtt
     2521 catcacgagg caaatccagt accggaataa tttcttcgta gatggctttt tcaacgaatg
     2581 cgcaaatttc agcatcgttc atcgcttcac ctacggtatc cagccctgcc tggaacgcca
     2641 ccggcaccag cgcggtgtgc gcaccgttga ggattgccac tttgcgttct ttatacggtt
     2701 taatatcgtc aacaatcagc acgttgagcg gatatttgtc caggcgcagc tcagtcgcta
     2761 aggatttcgg cccctgaatc acaaacaggt aaaagtgttc agcggtgtcg aggaaaccat
     2821 cgtgataacc cagttcttct tccagtttag ccacttcatc gcgcggataa ccggtaacga
     2881 tacggtccac cagcgtagaa cagaagctgt tagcctgatc cagccattga ataaatgctt
     2941 ctggcagtgc ccactcttgc gcatagcgca gcaccagttc acgcaacgcg tcgccattgt
     3001 agtcgatcaa ctcacacggg atgatgatcc aacctttatc cagcgcaccg ttgaaatggc
     3061 taaagcgttc gaacagcaga cgggtcagtt ttgccggata gcttatcgct ggcgcgtcat
     3121 cgaatttatc gcccgcatgg tagctgatcc ccgcttcggt ggtgttagag aaaacaaagc
     3181 gcatttccgg gttgtgcgcc agtttcagga attcatcgta ttcactgtag acgctgattt
     3241 cacgattaac cgagcgaatc agacgcgcgt cgctgaccgc ttcccccttc tcattcaggc
     3301 cacggataat ggtggtatac agaccatcct gcgtgctcag tgacggcggg aatgaagttt
     3361 caatcggacg aacaacgacc acgccagaat tcagatcggt gtgctcattc aggagatcga
     3421 tttgccagtc aacaaaggcg cgcaggaagt taccttcacc aaactgaatg atacgttctg
     3481 gatactgtgc accgggaaaa tcgcgacgat ttagtgtttt cacaatgggt tcccttctga
     3541 ttagtcatac aacctgtttg aattggtacg acaggttagc aaactttaat acgccgaacc
     3601 cctgttttga tcaactcctg atgattaatg agcagtttta tgagaaaagt gtggcgcgga
     3661 tcatggttta atcgaggaaa tacgcctttt cctggatcat aaagtggtag aacacattgc
     3721 attcaaatcg cgcggaatga ataaagatgt cagacaactt cctcaccgta acgcatagtg
     3781 ctggtacggt tgcgcccatc tttcttcgag cgatacagac acgtatcggc ttcgaccatc
     3841 aatttattaa aatcatcggt cagcgttcgg tacgatgcgc gaccactacc gacgccaata
     3901 cttaccgtga gataaagcgt tttttgttgc caggtgaatg gttgcagttc aacgccttta
     3961 cggatttttt ccgccattag cagaccatcg acaggattca ccgacggcac tgcaacagca
     4021 aattcttcgc cgcccatgcg cgccaccagc cccttatcac cgacaatctt ctgaatatgc
     4081 cgggcaaaca cgcttaacac tttatcgcca cattcatgtc catagttatc gttaatgctt
     4141 ttgaaatagt cgatatcaag cagcatgacc gtcagaggca cgatcaacac gcccgttgag
     4201 aattgctcac tgaaccagtc cgcaagcaaa ggccagaaat ccagactatc aatactgacc
     4261 gaaccaatcg ccccgacaat agcgcataat aatgcggcaa tcagacagta attaaatagc
     4321 cgtaaggcac ttactggctc atacttattt ttccccagac gcttatcccg cacgaccagt
     4381 aaggcgacgg taacaataaa catcatattg gagaaattga taaccagtga aaccagtccc
     4441 cattcggtgg ttatggcatc ataaaccagc atcgccacat aactgatcgc atagtaatgc
     4501 agtcgattaa gccagacata gcgggcaaac ccccccgcca ttacgccatt cagcggccag
     4561 aaaagagaaa gttcgtgaac cagccgtaac tctgcaccaa taaaatagaa cagcgtggtt
     4621 agcgcaaaaa tagcgatgct attacgtagc agatgacctt cctggaacaa acggaaagtt
     4681 gaaatggggt gtacatgcat taattatctg tccatacaat tgcgggtggc aattatctca
     4741 acgtaataag taaattctca taaaggatag catgttcgct aacagcggcg gcaaataaag
     4801 gtaccctggg aatgctattt tatcgttttc cgcaattctc atggctgatt catctgtaca
     4861 accagccagt gatagattac cgccacgata taacgttgtt gttcttcgct taacgaaaca
     4921 ccctgcggaa tgttatgcca cttagccaga caaccgcgac agcaggtcgc ggtggcgtgc
     4981 tgagcgataa acaccggatg cccgcgcatg ggggtttgct taccgtcatt agccggtaat
     5041 gccg
//
LOCUS       DAJVRF010000209         4803 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJVRF010000209 DAJVRF010000000
VERSION     DAJVRF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.209"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(279..1322)
                     /locus_tag="OQN13_003790"
     CDS             complement(279..1322)
                     /locus_tag="OQN13_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCS1617016.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            complement(1734..2918)
                     /locus_tag="OQN13_003791"
     CDS             complement(1734..2918)
                     /locus_tag="OQN13_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1617017.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(3218..3312)
                     /locus_tag="OQN13_003792"
     tRNA            complement(3218..3312)
                     /locus_tag="OQN13_003792"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3276..3278),aa:Sec,seq:tca)
     gene            3605..>4803
                     /locus_tag="OQN13_003793"
     CDS             3605..>4803
                     /locus_tag="OQN13_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCS1617018.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
BASE COUNT         1253 a         1055 c         1092 g         1403 t
ORIGIN      
        1 gggataccag ttcaaccgaa aacgccagag gaggggatta cccgctggca gggtaaatct
       61 gtggtttttg atgccagcta taacgtgttt gacgataaag tattacgaaa gaactttaat
      121 gagttcatgg agtaattcgt taaagtaatt tacaacgcta tccttttgca aaggaaactt
      181 tcagaaaata accccagggt aatcacctgg ggaaggaaca cagtgttatt attggataat
      241 aaattattac ttgttttaaa gttacactaa tgcaatatct actgatactg gatgcttgaa
      301 tgtttcatcc ctgctgcgga aaagggcaat atccagggcc agatatattc accgttcata
      361 tttctgcatt tctgtacaat ttcctcagaa agtgtcttgt caattctttt ccacaggtgc
      421 tcctgctgtc ttaaaatact ttcgcatatt tcgaaatcat cttcctgaat cagcatttcg
      481 gcgcatttaa tgatttctga tcgttcatgc agcacggagg aatggttata gagccagttt
      541 acaaacagca atgcttccgg atattgtccc cataatggtg agtatgttgc atagtaatct
      601 tctgccgtat ccagagagcc ggagaagtca tgccacacag caagtggaaa tggatgcaga
      661 tttccgcaca gcatttctgc gatgtaagtg tttgtggcaa agccatggcg aaaagctgtg
      721 gcggccttaa catgttcccc gttcagaaaa tgcaggagac cgagttcata ccagtaaggt
      781 gaaaattcat cagcatgctc tttcagtaca ctgaaggctc gctcatgatc gccggtgcgt
      841 aacagctctg aacccaacaa ccagcgagcc ccctgattat cttcaggatt gtatgccagt
      901 attttatcag tgagcataac cgcatcctga tggcgctgta agtgcacatt tgcgagaatt
      961 gctgcataaa gcgcgcgcag ataaggccgg ttttcgggat gcatccagat tatttcacca
     1021 cagaaacttt ccgggatgat acgattacct gccgccagtc ctttaagaga ggcgttcagt
     1081 gccttacgcg gggcattctg ttccagaaat gcatatgcca gatgagcgtg tatatcaata
     1141 aagtccggct cccgttgagc aaggcgtcgg agtttgttga tgtaactcgt ctcgctgatt
     1201 tcatcagcgt cgtatgcatc aagcacttct tcaaactccc ccagcaatgg gccaaaccag
     1261 gtatccggaa atacgaaata acccttgtta tcgtgcactt caaagcataa tctatcgttc
     1321 atttctgagc ctgtaaaaca tgtggtgata tagtctgaga gcataaaagt tgagattctg
     1381 tgtatcaagt cctgtcttgg caccgaatga atattgctta cgaagcccct tcatgctttc
     1441 cttgagaaca ataattttat ctttatgtga atctcatttt ttataatgat gtcagatgat
     1501 tgaaatgcta aattatatct gaatcaactt ttcctttgct aaaggtatcg aaataactgc
     1561 aaggctgtat tttgtaattg ccaatttaaa aatgtcaatt tgtttttaat tatgtttttg
     1621 taataagttt gaagacgcaa aaaaaataat ataataacaa aaatcctcca gccttgtagg
     1681 ccagaggata aaatacttaa ttgaggaaaa tatcagggaa aagtatttct gacttaactt
     1741 tcccggctgg agagtgaatc aatgtaatca gcgtaccatt gcatcatttc acggcggcca
     1801 tcgagataca gggcatgatt ataggtaccc ctgatggaat ttttatccac atgagctaac
     1861 tgcatttcaa tccaggcgga attaaagcct tgttcatgca gaattgtact catggtgtgt
     1921 ctgaaaccgt gccctgtgag tcttccctta tatccaagta attcaattac ttggttaatg
     1981 ctttccttac taattggttt gcggggatca tttctaccga taaagactag gggatgatgt
     2041 tttgagattg gttcaagctg cttaaatagc ataatgacct gctcagagag cggcacaata
     2101 tgaggtcgct tcatcttcat tacttctgcc gggatctccc acaattttgt ctcaaaatcg
     2161 atatcttccc agcgtgcaaa acgcagttct tgtgttcgta cgccggttaa cataatgatc
     2221 tgagtagctg tcttagtgat tatacttccg gtataacccg ccagatcgtt gaggaagtgg
     2281 ggaagttcat tagcagtaag aaagggaaaa tgtacttttt tcggtgtagc taaagcactg
     2341 gcaagatcgg gggctgggtt atagtctgcc cggccagtaa caattgcata gcggaacacc
     2401 tcaccacagc gctggcggac cttgcgcatt ttctccagag ctccacgttt ttccatttta
     2461 cgcagtgctt ccagtaactc cattggtttg atttcggcaa tagggcgctt gccaatgtag
     2521 ggaaaaatgt ctttttcaaa ggtatcaatg atttcatcac gatagcgtaa tgaccaccga
     2581 tcagcttttg actggtgcca ttcgcgagca atagcctcga acgtattttt cagactcatt
     2641 tgctgcgcga ttttttcttc cttttttgcc tcccctggat cgcctccagc agcgaggagt
     2701 ttcctggctt cactgcgttt ctcgcgagca tcggctagcg ttacatcggg gtatacgcca
     2761 atagaaagca tcttttcttt accagcaaag cggtacttca tgcgccagta tttagaacca
     2821 ttagtgttaa tcagtagata cataccgccg ccatcagcaa gcttataggg tttgtctttg
     2881 ggttttgcgg ttttcacttt gatatcggtt aatgccatga catcctccgg cttttgatga
     2941 attcatgttc ctgaggggga tttctgccag taactaacct gataccccca atacacccct
     3001 tgtataacaa agatattgtt aaaagccgtt gttatgtatc acatttttcc tattatgggg
     3061 gtgcttgtat ttcaatagga aataacaccc cctaaagtac ccccaaaaga aacttgatat
     3121 ggggtgaagt aggttgacct caggatacaa ataaatactg atatacaagg gattagaaga
     3181 gatttattga cattgggaga gttcagaaga ggtaaactgg cggaagatca caggagtcga
     3241 acctgcccgg gaccgctggc ggccccaact ggatttgaag tccagccgcc tcaccggaga
     3301 cgacgatctt ccgcgcctcg attgctacat ggaggcgggg cgcattatag ctacttcctt
     3361 gagtttctac atcccccaga tcagtttttt ccgcctaata cacgcagcgt attatcacga
     3421 aagcattata actcatataa aacccatatt ttacaatgat atatgagcgg aattaacgct
     3481 accacgatca cataaaacaa gaatcataaa ttaataacca gatatcggaa tattcgctct
     3541 cacagggatg gtttacaaaa tgcgttatcg gtctcagcac ccctatagca tcaaggaaaa
     3601 gcagatgaag agtgaagtgt tgtccgttaa agagaaaatt ggttatggca tgggagacgc
     3661 cgccagccac attattttcg ataacgtaat gttatatatg atgttctttt ataccgatat
     3721 ttttggcatt cctgccggat ttgtcggaac catgtttttg gtcgctcgtg cactggatgc
     3781 gatttccgat ccttgcatgg ggttgttggc cgatcgaacg cgctctcgct ggggtaaatt
     3841 tcgtccgtgg gtactgtttg gcgcactgcc attcgggatc gtctgtgtac tggcctatag
     3901 cacgccagat ctcagtatga acggcaaaat gatctatgca gcaattactt acaccctact
     3961 taccttactt tataccgtcg tcaatatccc ttactgcgca ttgggtggtg taatcaccaa
     4021 tgacccgact cagcgtatct cgctgcaatc ctggcgtttt gtgctggcga ccgcgggagg
     4081 catgctttct actgttctga tgatgccact ggttaattta attggcggtg ataataaacc
     4141 actcggtttc cagggcggta tcgcggtcct ttccgtggtg gcattcatga tgctggcatt
     4201 ttgtttcttc accactaaag aacgcgttga agcaccacct acaacaacgt ctatgcggga
     4261 agatttacgt gatatctggc aaaacgacca gtggcggatt gtcggtttac taaccatttt
     4321 caatatcctg gcggtgtgcg tacgcggtgg ggcgatgatg tattacgtca catggatttt
     4381 gggcacgccg gaagtgtttg tcgcttttct caccacttat tgcgtgggta acctgattgg
     4441 ttccgcactg gcaaaacctc tgaccgactg gaaatgtaaa gtcactatct tctggtggac
     4501 gaacgccctg ctggcagtga ttagcctcgc gatgttcttt gttcccatgc aggccagcat
     4561 cactatgttt gtcttcatct tcgtgattgg tgtgttgcat caactggtga cacctatcca
     4621 gtgggtaatg atgtccgata ccgtcgacta cggcgagtgg tgcaatggta aacgcctgac
     4681 cgggatcagt tttgctggca cgctgtttgt gctcaaactg gggttggcct tcggcggcgc
     4741 tcttatcggc tggatgctgg cttatggcgg atatgatgcg gcagaaaaag cgcagaacag
     4801 cgc
//
LOCUS       DAJVRF010000210         4796 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJVRF010000210 DAJVRF010000000
VERSION     DAJVRF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.210"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            6..81
                     /locus_tag="OQN13_003794"
     tRNA            6..81
                     /locus_tag="OQN13_003794"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="OQN13_003795"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="OQN13_003795"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="OQN13_003796"
     CDS             complement(299..1057)
                     /locus_tag="OQN13_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCS1617019.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTKAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="OQN13_003797"
     CDS             complement(1065..2168)
                     /locus_tag="OQN13_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCS1617020.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="OQN13_003798"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="OQN13_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..4452)
                     /locus_tag="OQN13_003799"
     CDS             complement(3427..4452)
                     /locus_tag="OQN13_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1617021.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTDYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1315 a         1238 c         1146 g         1097 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccttcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcatgg cgaaaagcgt
     3721 ccagcgtaca atcgagaacc attcatcatc gccacgacga actaccggcc caagcggttc
     3781 tttagagata acctccggta agacaatcca ttcagctggg ttgcttaatt tgatgcgcag
     3841 ggcatacagt tgcgactgat ccgaggccag cgtatcgcag cgaccagatt ccagtgcctt
     3901 cgctgattcg tcagagcgat cgaaagtcac cggtgtgtac ttcatattgt ttgccttgaa
     3961 atagtcggcg acgttgagtt cggtatctgt acccgcctga atacagacgg tagcgccatc
     4021 cagttctttc gcgcttttta gccccgcttt atcgtgcgtc aggaagccaa tgccgtcgta
     4081 atcagtgacg ccagtaaatg ccattcccat cccggcatcg cgagatgaag tccaggtcgt
     4141 attacgggag agcaaatcca cctcccctga ctgtaaagcg gtgaagcgtt cttttgcagt
     4201 gagcggggta tatttcactt tcgtgtcgtc accaaataca gcagcggcaa caccacgaca
     4261 aacatctaca tcaatacctg aaaacttacc gtctgcatcg gcataagaga acccaggtaa
     4321 tccatcactg atcccgcatt gcacaaaacc ttttttctgt acggcatcaa gcgtcgcgcc
     4381 agcatgcgcc tgatttgcaa cggcaagcag cacgctggcg gcagccagtg tggctatcat
     4441 catctttttc ataatgcttc ctgtgaggcg aaaattatcg ttataggacg tttgggaaac
     4501 gtcttttacc tgtctgtggc catcctctta taagcaaaag tagtgccatc ttttgcgcac
     4561 gatttcgcgt cgcaataagc tgagtgtaga caggaaataa taaaaacagc gccccgcaat
     4621 ggtgcgaaaa tgaaacatgc gccacatttt ggcgcaaaat atttacgcgt gttttactaa
     4681 gcagaataat gattgttagc gtgagtatat ttctggtggg aggtgtgaaa gttaaattga
     4741 tgcgttattt cgaaagcagc gaaataacgc gatataacat gctaaggtaa tgactc
//
LOCUS       DAJVRF010000211         4775 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJVRF010000211 DAJVRF010000000
VERSION     DAJVRF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.211"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(51..494)
                     /locus_tag="OQN13_003800"
     CDS             complement(51..494)
                     /locus_tag="OQN13_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCS1617022.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            651..1580
                     /gene="metA"
                     /locus_tag="OQN13_003801"
     CDS             651..1580
                     /gene="metA"
                     /locus_tag="OQN13_003801"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCS1617023.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1849..3450
                     /gene="aceB"
                     /locus_tag="OQN13_003802"
                     /pseudo
     CDS             1849..3450
                     /gene="aceB"
                     /locus_tag="OQN13_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3480..4771
                     /gene="aceA"
                     /locus_tag="OQN13_003803"
                     /pseudo
     CDS             3480..4771
                     /gene="aceA"
                     /locus_tag="OQN13_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1189 a         1257 c         1208 g         1121 t
ORIGIN      
        1 tgcgacgctg gcgcgtcttc tccagcctgg gcaggcacgg caggggcagc ttattcctcc
       61 acatacgcca gattcagcaa cggatacggt ttccccaaat cgtccacctc agagcgtccc
      121 gtaaccttaa aacccacctt cttatagaac ccaaccgcct gctcattttg ctcattaacg
      181 ttggttgtca gttccggtgc catcgagagc gcatgctcca ccagcatccg acccacgccg
      241 cagccgcaca catcaggatc gataaacagc gcatccatat gctgcccact taacaacata
      301 aacccaaccg gctgatcccg ttcattaacc gcgacccaca acggcgcttc cggcaggaag
      361 gaacgaacta gcacctccag ctcggcccga tactctgctg ataaaaaatc gtgtgtggca
      421 tcgacagaac gacaccaaat cgcaacgagt tcctcccctt cctcatgccg tgagcggcga
      481 atactaataa ccattttctc tccttttcgt cattcttata ttctaacgta gccttttcct
      541 tgaaactttc tcaccttcaa catgcaggct cgacattggc aaattttctg gttatcttca
      601 gctatctgga tgtctaaacg tataagcgta tgtagtgagg taatcaggtt atgccgattc
      661 gtgtgccgga cgagctaccc gccgtcaatt tcttgcgtga agaaaacgtc tttgtgatga
      721 caacttctcg tgcgtctggt caggaaattc gtccacttaa ggttctgatc cttaacctga
      781 tgccgaagaa gattgaaact gaaaatcagt ttctgcgcct gctttcaaac tcacctttgc
      841 aggtcgatat tcagctgttg cgcatcgatt cccgtgaatc gcgcaacacg cccgcagagc
      901 atctgaacaa cttctactgt aactttgaag atattcagga acaaaacttt gacggtctga
      961 tagttaccgg cgcgccgctg ggcctggtgg agtttaatga tgtcgcttac tggccgcaga
     1021 tcaaacaggt gctggagtgg tcgaaagatc acgtcacctc gacgctgttt gtctgctggg
     1081 cggtacaggc cgcgctcaat atcctctacg gcattcctaa gcaaactcgc accgacaaac
     1141 tctctggcgt ttacgagcat catattctcc atcctcatgc gcttctgacg cgtggctttg
     1201 atgattcatt cctggcaccg cattcgcgct atgctgactt tccggcagcg ttgattcgtg
     1261 attacaccga tctggaaatt ctggcagaga cggaagaagg ggatgcatat ctgtttgcca
     1321 gtaaagataa gcgcattgcc tttgtgacgg gccatcccga atatgatgcg caaacgctgg
     1381 cgcaggaata tttccgcgat gtggaagccg gactagaccc ggatgtaccg tataactatt
     1441 tcccgcacaa tgatccgcaa aacaaaccgc gcgcgagctg gcgtagtcac ggtaatttgc
     1501 tgtttaccaa ctggctcaac tattacgtct accagatcac gccatacgat ctacggcaca
     1561 tgaatccaac gctggattaa tcttctgtga tagtcgatcg ttaagcgatt cagcacctta
     1621 cctcaggcac cttcgggtgc cttttttatt tccgaaacgc acctcagcaa gtgaataaat
     1681 tttattcata ttgttatcaa taagttatca agtattttta attaaaatgg aaattgtttt
     1741 tgattttgca ttttaaatga gtagtcttag ttgtgctgaa cgaaaagcgc acaacgatcc
     1801 ttcgttcaca gtggggaagt tttcggatcc atgacgagga gctgcacgat gactgaacag
     1861 gcaacaacaa ccgatgaact ggctttcaca aggccgtatg gcgagcagga gaagcaaatt
     1921 cttactgccg aagcggtaga atttctgact gagctggtga cgcattttac gccacaacgc
     1981 aataaacttc tggcagcgcg cattcagcag cagcaagata ttgataacgg aacgttgcct
     2041 gattttattt cggaaacagc ttccattcgc gatgctgatt ggaaaattcg cgggattcct
     2101 gcggacttag aagaccgccg cgtagagata actggcccgg tagagcgcaa gatggtgatc
     2161 aacgcgctca acgccaatgt gaaagtcttt atggccgatt tcgaagattc actggccccg
     2221 gactggaaca aagtgatcga cgggcaaatt aacctgcgtg atgcagttaa cggcaccatc
     2281 agctatacca atgaagcagg caaaatttat cagctcaagc ccaatccagc ggttttgatt
     2341 tgtcgggtac gcggtctaca cttgccggaa aaacatgtca cctggcgtgg tgaggcaatc
     2401 cccggtagcc tgtttgattt tgcgctctat ttcttccaca actatcaggc actgttggca
     2461 aagggcagcg gtccctattt ctatctgccg aaaacccagt cctggcagga agcggcctgg
     2521 tggagcgaag tcttcagcta tgcagaagat cgctttaatc tgccgcgcgg cgccatcaag
     2581 gcgacgttgc tgattgaaac gctgcccgcc gtgttccaga tggacgaaat ccttcacgcg
     2641 ctgcgcgacc atattgttgg tctgaactgc ggtcgctggg attacatctt cagctatatc
     2701 aaaacgttga aaaactatcc cgatcgtgtc ctgccagaca gacaggccgt gacgatggat
     2761 aaaccattcc tgaatgctta ctcacgcctg ctgattaaaa cctgccataa acgcggtgct
     2821 tttgcgatgg gcggcatggc agcgtttatt ccgagcaaag atgaagagcg taataaccag
     2881 gtgctcaaca aagtaaaagc ggataaagcg ctggaagtca ataacggtca cgatggcaca
     2941 tggatcgctc acccaggcct tgcggacacg gcaatggcgg tattcaacga cattctcggc
     3001 tcccgtaaaa atcagcttga agtattgcgc gaacaagacg cgccgattac tgccgatcag
     3061 ctgctggcac cttgtgacgg tgaacgcacc gaagaaggta tgcgcgccaa cattcgcgtg
     3121 gctgtgcagt acatcgaagc gtggatctcc ggcaacggct gcgtgccgat ttatggcctg
     3181 atggaagatg cggcgacggc tgaaatttcc cgtacctcga tctggcagtg gatccatcat
     3241 caaaaaacgt tgagcaatgg caaaccggtg accaaagcct tgttccgcta gatgctgggc
     3301 gaagagatga aagtcattgc cagcgaactg ggcgaagaac gtttctccca gggccgtttt
     3361 gacgatgccg cacgcttgat ggaacagatc accacttccg atgagttaat tgatttcctg
     3421 accctgccag gctaccgcct gttagcgtaa accaccacat aactatggag catctgcaca
     3481 tgaaaacccg tacacaacaa attgaagaat tacagaaaga gtggactcaa ccgcgttggg
     3541 aaggcattac tcgcccatac agtgcggaag atgtggtgaa attacgcggt tcagtcaatc
     3601 ctgaatgcac gctggcgcaa ctgggcgcag cgaaaatgtg gcgtctgctg cacggtgagt
     3661 cgaaaaaagg ctacatcaac agcctcggtg cactgactgg cggtcaggcg ctgcaacagg
     3721 cgaaagcggg tattgaagca gtctatctgt cgggatggca ggtagcggcg gacgctaacc
     3781 tggcggccag catgtatccg gatcagtcgc tctatccggc aaactcggtg ccagctgtgg
     3841 tggagcggat caacaacacc ttccgtcgtg ccgatcagat ccaatggtcc gcgggcattg
     3901 agccgggcga tccgcgctat gtcgattact tcctgccgat cgttgccgat gcggaagccg
     3961 gttttggcgg tgtcctgaat gcctttgaac tgatgaaagc gatgattgaa gccggtgcag
     4021 cggcagttca cttcgaagat cagctggcgt cagtgaagaa atgcggtcac atgggcggca
     4081 aagttttggt gccaactcaa gaagctattc agaaactggt cgcggcgcgt ctggcagctg
     4141 acgtgacggg cgttccaacc ctactggttg ctcgtaccga tgccgatgca gcggatctga
     4201 tcacctccga ttgcgacccg tatgacagcg aatttattac cggcgagcgt accagtgaag
     4261 gcttcttccg tactcatgcg ggcattgagc aagcgatcag ccgtggcctg gcgtatgcgc
     4321 catatgctga cctggtctgg tgtgaaacct ccacgccgga tctggaactg gcgcgtcgct
     4381 ttgcacaagc tatccacgcg aaatatccgg gcaaactgct ggcttataac tgctcgccgt
     4441 cgttcaactg gcagaaaaac ctcgacgaca aaactattgc cagcttccag cagcagctgt
     4501 cggatatggg ctacaagttc cagttcatca ccctggcagg tatccacagc atgtggttca
     4561 acatgtttga cctggcaaac gcctatgcct agggcgaggg tatgaagcac tacgttgaga
     4621 aagtgcagca gccggaattt gccgctatac cttcgtatct caccagcagg aagtgggtac
     4681 aggttacttc gataaagtga cgactattat tcagggcggc acgtcttcag tcaccgcgct
     4741 gaccggctcc actgaagaat cgcagttcta agcaa
//
LOCUS       DAJVRF010000212         4727 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJVRF010000212 DAJVRF010000000
VERSION     DAJVRF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.212"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            496..1845
                     /gene="ghxP"
                     /locus_tag="OQN13_003804"
                     /gene_synonym="yjcD"
     CDS             496..1845
                     /gene="ghxP"
                     /locus_tag="OQN13_003804"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCS1617024.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            1997..3646
                     /locus_tag="OQN13_003805"
     CDS             1997..3646
                     /locus_tag="OQN13_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCS1617025.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(3800..4727)
                     /locus_tag="OQN13_003806"
                     /pseudo
     CDS             complement(3800..4727)
                     /locus_tag="OQN13_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1025 a         1103 c         1253 g         1346 t
ORIGIN      
        1 atcccgctgg tacacaggag ggtttccccc gacgtcaaca caccccattc gagcacgtgg
       61 tggaggttcc ggttggtgtt gatgctttaa ttgtatgtca ccggcatttc ttcgccagtg
      121 taaaagtata ctttttatcc gcaatatttt tgtcatctca gacgattttt tatcgcaatc
      181 ctgaacggta tacggctcga taacgctgca atcttgcgca ccgacgataa cgtttgcgca
      241 tcaattgcct ggtttttcat cgtcaagaca ataaaagaga aaaaagcagc aaacttcggt
      301 tgaaaaagcc gctatgatcg ccggataatc gtttgctttt tttaccaccc gttttgtatg
      361 cgcggagcta aacgtttgct tttttgcgac gcagtaaatt gtcgcaaacc tggagcagga
      421 agataacgtt tctctggcag gggattgtcc gccacgcatc ttgacgaaaa ttaaactctc
      481 aggggatgtt ttcttatgtc tacgccatca gcgcgtaccg gcggttcact cgacgcctgg
      541 tttaaaattt cacaacgtgg aagcactgtc cgtcaggaag tggttgccgg gttaacaacg
      601 tttctggcga tggtctactc ggtcatcgtc gttccaggta tgttgggtaa agcaggcttc
      661 ccgcctgcgg cagttttcgt tgcaacctgt ctggttgccg gactcggttc tatcgtgatg
      721 ggtctgtggg ctaatctgcc gttggcgatt ggttgcgcca tctccctgac ggcgtttacc
      781 gcattcagcc tggtgctggg gcaacatatt agcgtacctg tcgcgctggg tgccgtgttc
      841 ctgatgggtg tgctgtttac ggtaatttct gccacgggta tccgtagctg gattttgcgc
      901 aacttgccgc acggtgtggc gcacggcacg gggatcggta tcggtctgtt cctgctgctc
      961 attgccgcta atggtgtcgg tctggtgatt aaaaacccgc ttgatggtct gcccgttgcg
     1021 ctgggtgatt tcgcgacctt cccggtgatt atgtcactgg taggtctggc ggtgatcatc
     1081 ggcctggaaa aactgaaagt ccctggtggc attctgctga ccattatcgg tatctcaatt
     1141 gtcggtttga tcttcgatcc taacgtccat ttctccggcg ttttcgccat gccttcattg
     1201 agcgatgaaa acggcaattc actgattggc agcctggaca ttatgggcgc gctgaatcct
     1261 gtagtcctgc caagcgttct ggcgctggtg atgacggcag tatttgatgc caccggaact
     1321 atccgtgccg tcgccggcca ggcgaacctg ctggataaag atgggcagat catcgacggt
     1381 gggaaagcac tgaccactga ctccatgagc agcgttttct ctggcctggt gggtgctgct
     1441 ccggcagcgg tatacatcga gtctgcggcg ggtacggcgg cgggcggtaa aaccggtttg
     1501 acggctatca ccgttggcgt gctgttcctg ctgattctgt tcctctctcc gctctcttac
     1561 ctcgttccgg ggtatgcaac ggctccggcg ctgatgtacg ttggcctgct gatgctgagc
     1621 aacgtggcga aaatcgactt tgctgatttt gttgatgcga tggcgggtct ggttacggcg
     1681 gtattcatcg tgctgacctg taacatcgta acaggcatca tgatcggctt cgcgactctg
     1741 gtgattggtc gtctggtttc cggcgaatgg cgcaagttga acatcggtac ggtcgttatc
     1801 gccgtggcgc tggtgacctt ctatgcgggt ggctgggcta tctaatcttt catcggattc
     1861 tgaaaacggg tggcaatggc tgcccgtttt tattttctcc gcacattgtg tgagcttttg
     1921 cgatattctg aaaaaatgag aattcaggca taacgccttt ctcagatcat aaaaaaacat
     1981 cgcaaacagg gaacgcatgg aaatcttctt caccatactg ataatgaccc tcgtggtctc
     2041 actgtccggg gtggtcactc gtgtcatgcc ctttcagatc ccgcttccgc ttatgcaaat
     2101 cgccatcggt gcgctactgg cgtggccgac gtttggtttg catgtggagt ttgatcctga
     2161 actcttttta gtcttgttta tcccgccgtt gctgttcgct gatggctgga aaacgccgac
     2221 ccgtgaattt ctcgaacatg gtagagagat tttcggcctc gcgctggcgc tggtggtggt
     2281 caccgtggtc ggcattggct tccttattta ctgggtggtg ccgggcattc cgctgatccc
     2341 cgcctttgcg ctggcggcgg tactttctcc gaccgatgct gtggcgctct ccgggattgt
     2401 tggcgaaggg cgcatcccga aaaaaatcat gggcattttg cagggcgaag cgttgatgaa
     2461 tgacgcctcc ggcctggtgt cgttgaagtt tgccgtggca gtggcgatgg ggacgatgat
     2521 cttcaccgtc ggcggtgcga cggtcgaatt tatgaaagta gccattggtg gtattcttgc
     2581 cggttttgtg gtgagctggc tgtatggtcg ctcgctgcga ttcctcagcc gctggggcgg
     2641 tgatgaaccc gcgacgcaga tcgttctgct gttcttgctg ccattcgctt cttatctgat
     2701 tgccgaacat attggcgttt ccggcatcct cgctgcggtt gccgccggga tgaccatcac
     2761 ccgctccggt gtgatgcgcc gtgcgccgct ggcaatgcgc ctgcgcgcaa acagcacctg
     2821 ggcgatgctg gaatttgtct ttaacggcat ggtgttcctg ctgttaggtc tgcagctgcc
     2881 gggtattctg gagacgtcgc tgatggcggc agaaatcgac cctaacgtcg aaatctggat
     2941 gctgtttacc gatattattc tgatatatgc ggcgctgatg ctggtccgtt tcggctggct
     3001 gtggacgatg aaaaagttca gcaaccgctt cctgaagaag aagccgatgg agtttggttc
     3061 gtggaccaca cgagaaatcc tgatcgcgtc tttcgccggg gtgcgtgggg cgatcactct
     3121 ggccggtgtg ctctctatcc cgctgctctt gccggatggt aacgtcttcc cggcgcgcta
     3181 tgagctggtg ttcctggcgg ctggcgtcat tctcttctcg ctgtttgtcg gcgtggtgat
     3241 gttgcctatt ctgctacaac acattgaagt cgcggaccat tcgcaacaat tgaaagaaga
     3301 acgtattgcg cgagcggcaa cggcagaagt ggcgattgtg gcgatccaga aaatggagga
     3361 gcgtctggcg gcggataccg aagagaatat cgataaccag ctgcttacgg aggtcagttc
     3421 tcgcgtcatt ggtaacctgc gtcgtcgcgc cgatggacgt aacgacgttg aaagttccgt
     3481 gcaggaagag aaccttgagc gtcgcttccg tctggcggca ttgcgttctg aacgtgctga
     3541 actgtaccac ctgcgcgcca cgcgggagat cagcaacgaa acgctgcaaa aattactgca
     3601 cgatctcgat ttgcttgaag cgttgctaat tgaggaaaat cagtaagccg aaagcgacgt
     3661 aaccaggccc gaggtaatct gaaaaaggtg caatcttttg cacctttaac aggttagctc
     3721 gggctaacat tcatgattct aaaacaaata agttatctct cttgtaaagt caattcattg
     3781 gctgttcttc gacttaacct caatctgcgt gttgcataat atctgcagta atcttcatag
     3841 cgactgcatt aatattatcc tcagtttgtg acatgccagt atgagaggta caatcttctt
     3901 caaagaattt ctgtagtgtt accagggtat cactatcaat ggggatattt tcctccccac
     3961 ggagtatgga aatgcccatt ccctggacgc tagcctcatc aaaattaacc cgatagagac
     4021 gggtattaaa catacaggct tttgttaaaa tagaacagtt gaaatggcag ttgcttaaat
     4081 tggcattatt aaaattaaca ttagccatat tagagtcggt acatataacc atttcgagga
     4141 atgtatttgt tagccacgta caaaagagtt tgctatcaat taagtttata ttctggagga
     4201 ttgctttgtt cagattgacg cgagacagat cagcatgttc aaagttaatt aaatcgagtt
     4261 tagagaaact aaggtcggct ttaaataaat taacatatgg cgctattact ttataaagat
     4321 ttgcctcagc catgtacgcc atataaaaat ttgaatagtc aagaaaggta tttttaatat
     4381 tggctctatt cattgctgca ttgtagaatt gaacatgcat aacggagcat gcatttagag
     4441 ttccgtcggc catttttatc tgagcgaaat ttgagtcatc gagaatacaa ttatcaaaaa
     4501 gtatttttcc attatggcgt aataaaaatt cgttttatac atattacagt cctggaaaat
     4561 agcgttttgt agtctgcaac ctgcaaaatt cgaattggat aaatcttcat ctttaatcac
     4621 gatacctttc aggctaaggt gtgaaaaatc gtaaccacca tttactgtct gtggaatttc
     4681 gatagtatta ttatggacgg cgacattatt tataaaatat accacct
//
LOCUS       DAJVRF010000213         4572 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJVRF010000213 DAJVRF010000000
VERSION     DAJVRF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.213"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..854)
                     /gene="mhpB"
                     /locus_tag="OQN13_003807"
     CDS             complement(<1..854)
                     /gene="mhpB"
                     /locus_tag="OQN13_003807"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HCS1617026.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISA"
     gene            complement(856..2520)
                     /locus_tag="OQN13_003808"
     CDS             complement(856..2520)
                     /locus_tag="OQN13_003808"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HCS1617027.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            2562..3395
                     /gene="mhpR"
                     /locus_tag="OQN13_003809"
                     /pseudo
     CDS             2562..3395
                     /gene="mhpR"
                     /locus_tag="OQN13_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
     gene            3472..4554
                     /gene="lacI"
                     /locus_tag="OQN13_003810"
     CDS             3472..4554
                     /gene="lacI"
                     /locus_tag="OQN13_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HCS1617028.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
BASE COUNT         1058 a         1310 c         1261 g          943 t
ORIGIN      
        1 aatgcagaaa tagcggcaaa agcggcgacc caggttttga tttcatgtgt cgactttccg
       61 gcaatggcgg aaagctcttc gttactgacg gcatccagtt cctgtatgcg tccctgctcc
      121 agcaaagtca tgaactggtt atcccaaatc gggttgagcg gatgcagagt tctctgatcc
      181 tcaacaaact tctcagcggc gctaatcacc ctttgctgac gcaattcgcg ctcactggcg
      241 ggtaaatctt tcccgctccc caacagacgg tcgcgcatat gggcatcggc tttcgccagt
      301 tcgggcaccg gcggctgatg ggaaagccca ccggaaccca ggaacagcac gcgtttattg
      361 agagtgctgg tgaaacgtcc aatggcttca cccaacatgc gggtacgctg gaaaccgggc
      421 agcggcgtgg cgacaccgtt gatgaacaca ggcagaactg gcaccttatc cagcccaccg
      481 agcaggaact ccagcggctg ggcgaacccg tggtccacct gcatacagta agaaacggca
      541 agatcgatcc cgctcttcat gacagcatgc gcacaggcct ccgccagctc cacaggcacg
      601 ggcagctctc ctgccgcact gccgaaatca ccaattgccg tcgctccaac gcctaaacag
      661 aacggtggca tcacgtcata gaaaaagccg ttgtagtgat ctggcgcaaa cagcaccacc
      721 agttcagggg agaaagccgc aatacgctcg cgggcgctgg caatcacgcc attgacctca
      781 tcgagtacct cttgcgccgg gtcgacatac cccaccaacg gcgagtggga aagacagtga
      841 agataagcgt gcatatcagg ctaccttttc gacagaaacg tcggcatcag ggcgggtcag
      901 cgtcatcacc gacgccagtt tattcagcgt attgcccagg gtttgcggaa tggcggtggc
      961 ggcaacaaag cgatccgggc gcatcaccac cagcgaagca ttgtgttgcg cgaaccagct
     1021 acgcagacga ccttgcgtat cgcccacgcg tagcacgccg tcgtggttat cctgtgcggt
     1081 atgaatttgc acttccggca ccacctgaat gaagcgtgtg cccaacgcgc gccactgctg
     1141 gatttgctcg tcgctcatcc cccacagtgg attgcatccc cagccaatta ccgcgaagtt
     1201 cgcgccgatc gcgttatcga gcagcgtcac gtcgccgttt tccagcgtga ctttcggctg
     1261 aataaacatc ttgccgaccg gagagtgctt cgcttcgccc tcacgcacca gcgcaccgcc
     1321 gtaatattgc ggcatcggct tgaagcgcat ttcgaggaag tagcgtttta ctggcggcag
     1381 ataattcaac agccaggaaa cgccgtcacg taacgtaccc tgccagcgtt tcggcggagc
     1441 cagcacgttg cccgccgtca cggacaggtc aatcatcgct ttggcgtgat cgcgacgttc
     1501 ttgttgatag gtatcgagca gcgcatcgcg ggctttcccc tggataacca acgccagttt
     1561 ccaggcgagg ttaaaggcgt cgcgcatacc actgttatag ccctgcccct gccacaccgg
     1621 catgatgtgc gcggcatcgc ccgccagcag tacgcgatca atacggaaac gttgcgccag
     1681 tcgcgcgtta tgggtgtaga cacgctggcg aatcaattca acattgtccg gattaggcag
     1741 cactttgctt aacagcttgc gcatattttg cggctcacgc agctgctctt cggtttctcc
     1801 cggcatcacc ataaattcaa agcgacgtac cgcatgaggc agcgcggcag aaacatacgg
     1861 gcgcaccgga tcgcaacaca aatagatatg cggcgtactt aacggatcgt tggcgatatc
     1921 taccacaatc cactgatttg gcgcagtttt accttcaaac ggcacattca gggtgcgacg
     1981 aacaaaactt gccccaccat cacaggccac cagccactgg gctttgatcg tttcccgctg
     2041 cccttctgcc gttttcaggt gcaaggtcac ttcgtcatct tgctggctga aggcctccag
     2101 ctcgcgggaa aacaagcagc gcacattcgg aaaacgcgac aacccttcca gcatcaccgc
     2161 atcgacctgt ggctgaataa aggcgttacg gcgcggccag ccaaattcat cggtcattgg
     2221 ctgaatatca gcaaaacagc ggccttttgg ggtgagaaaa cgcatcgcgt gccacggtgt
     2281 agtgtgcggc agaacattct cgaccaggcc gaccgactgc atggtgcgca gcgcctcgtc
     2341 atcaatacca atcgcacgcg ggtagtcgat caacttatcg agtttctcca ccaccagcac
     2401 gtcaatgccc atctgaccga gatagttcgc catcatcagc ccaaccggac cggcaccagc
     2461 gatcgccacc tgaacgctat ggttaacagc aggctggatg tcagggtgtt gtattgccat
     2521 ttctgcacaa cgttgtttta actatagaaa tgtcaattaa tatgcagaac aatgagcaga
     2581 cggaatacaa aaccgtgcgc ggcttaaccc gcggtctaat gttattaaat atgttaaata
     2641 aacttgatgg cggtgccagc gtcgggctgc tggcggaact cagcggcctg catcgcacca
     2701 ctgtgcggcg actgctggag acgctgcagg aagagggata tgtccgccgt agcccctccg
     2761 atgacagttt tcgtctgacc atcaaagtgc ggcaattaag cgaaggattt cgtgacgaac
     2821 agtggatttc tgcactggcg gccccgctgc tgggcgatct gttgcgcgaa gtggtatgac
     2881 caacagatgt gtccaccctg gatgttgatg caatggtggt acgcgaaacc acccaccgtt
     2941 tcagccgctt atcctttcac cgggcaatgg tcgggcgacg tttaccgctc ctgaaaaccg
     3001 cctcgggcct gacctggctg gccttttgcc cggaacaaga acgcaaggaa ttaatcgaaa
     3061 tgttagccgc ccgccccggt gatgactatc aactggcacg agaaccgtta aagctgcaag
     3121 ccattctggc gcgcgcgcgc aaagagggtt acggacagaa ctaccgcggc tgggatcagg
     3181 aggagaagat cgcctctatc gccgtaccgc tgcgcagtga acaacgggtg attggctgtc
     3241 tgaatctggt gtatatggcg agcgcaatga ccattgaaca ggcagcggaa aagcatcttc
     3301 cggcgctaca acgggtagca aaacagatcg aagaaggggt tgaatcgcag gctattctgg
     3361 tggccggacg gcgaagcggc gtgcatttac gttgacacca tcgaatggcg caaaaccttt
     3421 cgtggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca
     3481 gtaacgctat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttctcgcgtg
     3541 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg
     3601 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg
     3661 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt
     3721 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc
     3781 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc
     3841 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt
     3901 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat
     3961 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg
     4021 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa
     4081 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg
     4141 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg
     4201 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc
     4261 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc
     4321 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc
     4381 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg
     4441 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     4501 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     4561 cgcaattaat gt
//
LOCUS       DAJVRF010000214         4433 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJVRF010000214 DAJVRF010000000
VERSION     DAJVRF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.214"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     misc_feature    <1..97
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            120..1280
                     /gene="pheA"
                     /locus_tag="OQN13_003811"
     CDS             120..1280
                     /gene="pheA"
                     /locus_tag="OQN13_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCS1617029.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEIFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(1323..2444)
                     /gene="tyrA"
                     /locus_tag="OQN13_003812"
     CDS             complement(1323..2444)
                     /gene="tyrA"
                     /locus_tag="OQN13_003812"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCS1617030.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(2455..3525)
                     /gene="aroF"
                     /locus_tag="OQN13_003813"
     CDS             complement(2455..3525)
                     /gene="aroF"
                     /locus_tag="OQN13_003813"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCS1617031.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            3734..4099
                     /locus_tag="OQN13_003814"
     CDS             3734..4099
                     /locus_tag="OQN13_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCS1617032.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4249..>4433
                     /locus_tag="OQN13_003815"
     CDS             4249..>4433
                     /locus_tag="OQN13_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCS1617033.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSR"
BASE COUNT         1172 a         1143 c         1018 g         1100 t
ORIGIN      
        1 tcttttttac cttcccctga atgggaggcg tttcgtcgtg tgaaacagaa tgcgaagacg
       61 aacaataagg cctcccaaac cggggggcct tttttattga taacaaaaaa ggcaacacta
      121 tgacatcgga aaatccatta ctggcgctgc gagagaaaat cagcgcgctg gatgaaaaat
      181 tattagcatt actggcagag cggcgcgaac tggccgtcga ggtgggaaaa gccaaactgc
      241 tctcgcatcg cccggtacga gatattgatc gtgaacgcga tttactggaa agattaatta
      301 cgctcggtaa agcgcaccat ctggacgccc attacattac tcgcctgttc cagctcatca
      361 ttgaagattc cgtattaact cagcaggctt tgctccagca acatctcaat aaaattaatc
      421 cgcactcagc acgcatcgct tttctcggcc ccaaaggttc ttattcccat cttgcggcgc
      481 gccagtatgc tgcccgtcac tttgagcaat tcattgaaag tggctgcgcc aaatttgccg
      541 atatttttaa tcaggtggaa accggccagg ccgactatgc cgtcgtaccg attgaaaata
      601 ccagctccgg tgccataaac gacgtttacg atctgctgca acataccagc ttgtcgattg
      661 ttggcgagat gacgttaact atcgaccatt gtttgttggt ctccggcacg actgatttat
      721 ccaccattaa tacggtctac agccatccgc agccattcca gcaatgcagc aaattcctta
      781 atcgttatcc tcactggaag attgaatata ccgaaagtac gtctgcggca atggaaaagg
      841 ttgcacaggc aaaatcaccg catgttgctg cgttgggaag cgaagctggc ggcactttgt
      901 acggtttgca ggtactggag cgtattgaag cgaatcagca acaaaacttc acccgatttg
      961 tggtgttggc acgtaaagcc attaacatgt ctgaccaggt tccggcgaaa acgacgttgt
     1021 taatggcgac cgggcaacaa gccggtgcgc tggttgaagc gttgctggtg ctgcgcaacc
     1081 acaatctgat tatgacccgt ctggaatcac gcccgattca cggtaatcca tgggaagaga
     1141 tattttatct ggatattcag gccaatcttg aatcagcgga aatgcaaaaa gcattgaaag
     1201 agttagggga aattacccgt tcaatgaagg tattgggctg ttacccaagt gagaacgtag
     1261 tgcctgttga tccaacctga tgaaaaggtg ccggatgatg tgaatcatcc ggcactggat
     1321 tattactggc ggttgtcatt cgcctgacgc aataacacgc ggctttcact ctgaaaacgc
     1381 tgtgcgtaat cgccgaacca gtgctccacc ttgcggaaac tgtcaataaa cgcctgctta
     1441 tcgccctgct ccagcaactc aatcgcctcg ccgaaacgct tatagtaacg tttgattaac
     1501 gccagattac gctctgacga cataataatg tcggcataaa gctgcggatc ctgagcaaac
     1561 agtcgcccga ccatcgccag ctcaaggcgg taaatcggcg aagagagcgc cagaagttgc
     1621 tcaagctgaa cattttcttc tgccagatgc agcccataag caaaagtagc aaagtggcgc
     1681 agagcctgaa taaacgccat attctgatcg tgctcgacag cgctaatacg atgcagccga
     1741 gcgccccaga cctgaatttg ctccagaaac cattggtatg cttccggctt acgtccatca
     1801 caccagacca caacttgctt tgccaggcta ccgctgtccg ggccgaacat cgggtgtaac
     1861 cccagtaccg ggccatcgtg cgccgccagc atggcctgta atggtccatt tttcactgat
     1921 gccagatcaa ccagaataca atctttcggt aaaggcggta atttgccgat aacttgctca
     1981 gtaacgtgga ttggcacact aacaatcacc attccggcat cggcaacaat atcagccgct
     2041 cgatcccagt catgttgctc cagaatccgc acctgataac ccgagagtgt cagcatcttc
     2101 tcgaacaggc gtcccatctg accgccaccg ccgacgataa ccaccggacg cagtgacgga
     2161 caaagtgttt taaatccttt gtcgttttca ctggagtaag attcacgcat cacccgacgc
     2221 aaaacatcct caatcagatc tggcggtaca cccagagctt ccgcctctgc acgacgcgag
     2281 gccaacatag atgcctcgcg ctccggaaca taaataggca gtccaaagcg gcttttcacc
     2341 tcgcccactt cagcaaccag ttccagacgc ttcgctaata aattcagcag cgctttatcg
     2401 acttcatcaa tttgatcgcg taatgcggtc aattcagcaa ccataataaa cctcttaagc
     2461 cacgcgagcc gtcagctgcc cgttcagatc ctgatgaatt tcacgcagca aggcatcggt
     2521 catttcccag ctaatgcagg catcggttac ggatacaccg tatttcattt cactgcgcgg
     2581 ttgctcagaa gactgattgc cctcgtggat attactttcg atcatcagac caataattga
     2641 gcgattgcca tctttgattt gagcaaccac ggattctgcc accgcaggct gacggcgata
     2701 atctttattg gaattaccgt ggctgcaatc taccatcaga gacgggcgca gtcccgcctg
     2761 ttccatctct ttttcacatt gcgcaacatc cgcagggcta tagttcggcg ctttaccacc
     2821 gcgcaggatg acatgcccgt ccggattccc ctgagtttgt agcaacgcaa cctgccctgc
     2881 ctggttaatg ccaacaaaac ggtgcggctg ggcggcggcg cgcatagcgt taattgctgt
     2941 tgccagactg ccgtcggtgc cgtttttaaa accaaccggc atggaaagcc cggaggccat
     3001 ttcacggtga gtttgcgatt ccgttgtacg agcaccaatt gctgaccagc taaacagatc
     3061 gcccaggtat tgcgggctat tcggatctaa cgcttccgtc gccagtggca gtcccatatt
     3121 caccagctca agcagcaatt tacgcgcgat ctgcagcccg gcttctacat caaaagagcc
     3181 atccatatgg ggatcgttaa ttaacccttt ccagccgaca gtggtacggg gtttttcaaa
     3241 atagacgcgc attaccagat agaggctatc gctgacctct gcggcaaggg ctttaaatcg
     3301 acgagcatat tccagagcag tttccggatc atgaatggaa caaggaccac aaactaccag
     3361 cagacgagga tcgcgcccgg cgataatatc tgaaatggtt ttacgcgagt cagcaatctg
     3421 ggcttcttgt tgcaggctca atggaaaagc ggccttcagt tgttccggag tcattaaaac
     3481 ctgttcgtcg gtaatatgta cgttattcag cgcgtctttt tgcatgatgg cgatcctgtt
     3541 tatgctcgtt tgcgatagtt gatcctcagc gaggatgacg taacgataac acataaagta
     3601 aagttttcaa tccatatttc gtacattttt ctttacacga acaatttgct cgcgcttttg
     3661 atgcttattt cgtacaaata aatttacacc tcctttaaaa acaatccgct atgctttgaa
     3721 aaaggagaaa gaaatgatga aaaagtttat cgccccctta ttggctttac tggttagcgg
     3781 atgtcagatt gatccttata ctcacgcgcc aaccttgacc agcaccgact ggtatgatgt
     3841 cggtatggaa gatgcgatat cgggcagcgc cataaaagat gacgatgcat ttagcgattc
     3901 acaggcggat cgcggtctat accttaaagg atatgccgaa ggacaaaaga aaacttgcca
     3961 gaccgatttt acttatgccc ggggactttc cggtaaaagc tttcctgcca gctgtaacaa
     4021 tgttgaaagt gccagccaac tgcatgaagt atggcaaaaa ggggctgatg aaaatgccag
     4081 cacaatacga ttaaattaat taaaacccca caaataaatt aaacataaga ttttactcat
     4141 tgtgttttat ttctcacatt gatgacgatc gcatgaaaaa atgatatttt aatgagcatt
     4201 ctgaaaaaat atcgatgcat ttcgagcgaa gatggtgggg atccctgaat gcgtttttct
     4261 caccgactgt tcttacttct gattctcctg ctgacgggag caccaatact cgctcaggag
     4321 ccttctgacg ttgcgaaaaa cgtgcgtatg atggtttccg gtattgtcag ttacacccgc
     4381 tggcctgcgc tatcagggcc accaaagtta tgtatctttt catcttcacg ttt
//
LOCUS       DAJVRF010000215         4399 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJVRF010000215 DAJVRF010000000
VERSION     DAJVRF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.215"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(139..3654)
                     /locus_tag="OQN13_003816"
     CDS             complement(139..3654)
                     /locus_tag="OQN13_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS1617034.1"
                     /translation="MDKNALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4106..>4399)
                     /locus_tag="OQN13_003817"
     CDS             complement(4106..>4399)
                     /locus_tag="OQN13_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1617035.1"
                     /translation="NKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQMS
                     HQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1139 a         1126 c          904 g         1230 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagatgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg ataccttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca ttaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcgtcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaggagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aatcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcacct gcagaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc ttaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt tggcgtcttt ccgttcaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggt aaaccctaaa gcatttttat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aaccaccata tggacaacta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcggtat caaatgtact
     3781 ggttgtacaa ggttcagaac acaagcacat tcaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa tgactgcctg tgttggacaa ggataccaga
     3901 acagccgaaa acaccggggg ttattacctg ctctcatgga aaccagatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacat gacgaaggga ctttaatgga agccggagta
     4021 acttgccaga acatcaatct tcgtcatcat ttacaaagaa aaataatcag ttaaagcagt
     4081 ccgggcaatc agcccggact gcttactatt ttaatggatt gttaatcttt gcatattcaa
     4141 acgggctgat acccttatct ctgttcgcat ccagaaaatc tgcccaccac tgtagcatca
     4201 gtcgacgttc ttccagatgt tctgctttat ggatatacgc agcacgtaca ccattacgct
     4261 cctgatggct catctgacgt tccacagcat cgcgggacca caaacctgat tcaatcaatg
     4321 cactgcacgc catcgcccgg aatccatggc cacagacatc ctgggttgtg tcatacccca
     4381 tcaccctcag ggctttatt
//
LOCUS       DAJVRF010000216         4329 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJVRF010000216 DAJVRF010000000
VERSION     DAJVRF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.216"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..582)
                     /locus_tag="OQN13_003818"
     CDS             complement(<1..582)
                     /locus_tag="OQN13_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1617036.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPNF"
     gene            complement(645..1094)
                     /locus_tag="OQN13_003819"
     CDS             complement(645..1094)
                     /locus_tag="OQN13_003819"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCS1617037.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1091..4183)
                     /gene="ebgA"
                     /locus_tag="OQN13_003820"
                     /pseudo
     CDS             complement(1091..4183)
                     /gene="ebgA"
                     /locus_tag="OQN13_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1029 a         1090 c         1168 g         1042 t
ORIGIN      
        1 gaagttcggg atcatcgcgt caacggtgat ggcgtcagca ggctgtacgc cgccaaccag
       61 cgccgtacct gccagtaaaa tgtaggagag cgttaacagc agcatcagcg ttgaagtgac
      121 ggaggtaatt ggccccaaca ttttcgcccc gttggtggaa acccaggtgg agaaggcgaa
      181 cagcaccata ctgataatgg tggtggcaac cggcgtcata atatattcgt atccgaggaa
      241 ggcatacgaa gcataagcaa taacgcgcgg caacagtgag gtgaaaaaga acaggtttac
      301 gaaccagtag gtataggcag taataaatgc ccaacgtccg cccagcgaac tttttaccca
      361 tgcgtagaca ccggcttctg agtttttatt taacgaaaca aattctgcga tgatcagaca
      421 gaagggaata aaataaaaaa tcgtcgcgag gaaaaacatc ggtgccgagg ccagtccaag
      481 atcaatatta ttattgataa cgttgttaaa gctgtaaacg gcggcaaaag tcagcgagag
      541 caagccgaat ttgccgattg tattacgttt ggtatcagac ataacacatc tccgaaaatg
      601 agtagggtag cggtggcggc aaactccttt tgccgccgta gttgttattt gttatggaaa
      661 taaccatctt cgatggtgac tttgagaacc acttttttga ccgcgttatt gcagataaac
      721 cgatacgctt catggatatc gcaaataacg atttgccctt cgtggacctc aacggtttct
      781 ccgcagcctt ttaaatattc acggtcagtt tcatcacgat aatattccac tacctgtaat
      841 gtctctttcg gcgcatattc aattttttgc tgcccttgca ggtagtaatg cacttcaaaa
      901 tagcgtcgat gcccggtaaa tagcgcatcg gtcgcggagt ctgtctccac gcggtaagtc
      961 aatgagtcac cgatggagtg ggcgacgcca ggctgaatgt tgtcgatatt ttcaatcgct
     1021 tcaacgcagc gttgccactt cttgccagag gcgtaaatct ggcggaactg ttctaagtta
     1081 tcgatgatcc tcattgctta ttctcgctgt gcaaattcgt ggaaaagaac cctgcgccga
     1141 actcatacga cgccaggctt tgcgcggtag cttctccgcc agaaaccggc agcaacgtaa
     1201 agccgtagct gaagtcacgg aaccagacgc gccaggagtc cagcacctcg ctgccccagg
     1261 agttggagcc gaggccaagc agctggtgat cgaggttcag ggtgatgtca tcactgcgct
     1321 gcagctcgtt acagtgctgg gcagcgtgga tgttttccta ggtatagtgc caggcgctga
     1381 agttaattgg gcgctgcgga accaccagca gaccgttacc gtggcggtta gtcagtgccg
     1441 tccagcggac atgctgacgg ttaccgttgt tctgcgggaa gggatagttc tcgaacatgg
     1501 catcgacggt gctgcgccag atatcgatga tgttagcctg ctggctgtcg gcgtagtttt
     1561 cgcccggtcc acgaccgtaa tacgccacct gatcgtattc gccgttaatt cccatggtga
     1621 aaccgatgca cggaatgatg tgcggatagt cgccgtaacg ctcgccggaa agcgccacgt
     1681 taacctggcc atcggcagcg atgcgccaga tgtaggtgca gcgcattccg aaatcaaaca
     1741 ccggcggggc aataaccgtg cggctgatga tcaacacttc accctcgctc tgttctacgg
     1801 caaagtcgcg cagatgttcc tgcatgatct gcaaatgatt cggctgccac aacccttcgt
     1861 actcctgctt gtggttgtcg atcatcggct tgaagaagtt gatctttggc tcgcgggtca
     1921 gcagcgactc gccatttacc tgccaggatg tcggtttgcc actcattttt gagaaggtga
     1981 tcgcgaagtt gtagccgcga acggtgcagc tcaaacgatc gtcttccagc gtcagcggac
     2041 gcgcattatt tggtgcgaaa ggcactggct gcgcggtgtt ttccttcagc gggaactgat
     2101 aagtggcgat agaatgcccg gcttcgctgt agcgggtgcg ggaatctttg gtcaccgtaa
     2161 tgttgaggaa cgcttcgcgg gcgtccagct gcggcagcgt gatctgcaag ggggcttcgc
     2221 tgttcggcgc aacgtcgcgc agtttaatct gctgcgtcgc gagcgtttca ccttcggcgc
     2281 gcacctctgc gtgcaggggg tagtcatcaa gcgtggtaaa ccacagttta ttttcgactt
     2341 tcaactcgcc gcgagtcaga tcccgcgcgt ggatttttac cggcgcgata acctgtttgt
     2401 actctttcag gcccggtccc ggcgtctgat cggaatagat caaaccatca agacagaagt
     2461 tatagttgtt gggatagtcg ccgtagtcgc cgccgaattt ataccagaca ttgccgtggt
     2521 cgtcctgtgc ctggatcccg tggtcgcacc actcccagac ataatgaccc tgaatgcaat
     2581 cgtgcttata gaagacgttc tggtactccg tcagcccgcc cggtccgttc cccatcgcat
     2641 gagcatattc acagatgatg cgcggcttcg gatgcgggta ttcaccaaac tcattcatca
     2701 gcggcacgcg ggtgtacatg gtggaaataa tatcgaccac ttcagcatcg cgatcttctt
     2761 cgtaatgcac cagtcgcgtg tcatccagcg ccttcgctgc gtggtacatc gcgcggatgt
     2821 tacagccata gccggattca ttgcccagcg accagatgat gatcgacgga tggtttttct
     2881 gcgcgtgaat atggcgaaca atgcgctcga cgtagacttt ttcccactgc ggatcgtcgg
     2941 taatacggct gatatcgccg acattagcaa agccgtgcga ttcgacgtcg gtttccgcca
     3001 tcacaaacag gccgtagata tcacacagtt cgtaaaaacg cggatcgttc gggtagtgag
     3061 cggtacgcac ggagttgatg ttgtgctgct tcatcaactg gagatctttc tcgacgcgat
     3121 ccattccaac ggcgcggcct ttgcgatgat cgttgtcgtg acggttgacg ccgtgcagca
     3181 tcacataacg gttattgatc cagaacagac cgtcgcgcac tttgatatca cggaagccaa
     3241 cgcgttgtgg taccacttcc agaacgttgc cgttggcgtc tttcagcgtc atgaccagat
     3301 ggtaaagata aggggattct gctgaccatt gctgcggctg ttcgacagta aaagcaaagc
     3361 tggcgctggt cagtttttca attgccaaat gatcaatggc gctgctgtgc accacgcgtt
     3421 cgccatcaaa cagggtatat tccagcgtcg tgacgacagg ggaggcggcg agattttcca
     3481 gcaccacttc gcaggaaagc gtggcatcgc aataggcttc gtcaaagtcg gtacgcacgg
     3541 tgaaatcgtt aatatgtgtt agttgttttc cgatcagata aacatcgcgg aagatccccg
     3601 ctgaccacca catatcctgg tcttccacgt aggtagagtc cgcccattgc atcacgcgca
     3661 cacacaacag gttgtcgccg gttttaacca tcgcgctgat gtcaaactct gcggtcaggc
     3721 gactgccctt gctgaaaccc acatactgac cgttaacgta gacttcaaaa taggtttcga
     3781 cgccgtcaaa tttaatcagc gtctgtttac cctgccagcc gtcgctgagg gtgaaaatac
     3841 gttgataggc accggttggg ttatcgctgg gaacaaacgg cacatcgatg gggaacggaa
     3901 aaccttcgtc ggtatattgc agtttgccgt gaccttccat ttgccacatg gcggggacgg
     3961 taatatgccc ccagtcagcc attaactcag aggtgaaggc ttcaggcact tgcagcgggt
     4021 ggtcaaaaaa gtggaaattc cactgaccgc ttaagggcag aaacaggctg ctggtttcgc
     4081 gggcaaaggt acgcgcttgc gcaacagaat catatgaaaa aaagtacgca cgtggcgcaa
     4141 gtcggttttc gtgggtgagc tgaatgtttt cccagcgatt catagggcct cccggtagag
     4201 agaaatcgga accattcatg gtgatggtag tttgtgtaat tgctggggag tttagtaaaa
     4261 tttttactaa aaagtgacag gtaaatgcat ttactttaac tggatcacga aaataaacgg
     4321 cagcactgt
//
LOCUS       DAJVRF010000217         4249 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJVRF010000217 DAJVRF010000000
VERSION     DAJVRF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.217"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..622
                     /gene="phnP"
                     /locus_tag="OQN13_003821"
     CDS             <1..622
                     /gene="phnP"
                     /locus_tag="OQN13_003821"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCS1617038.1"
                     /translation="NEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRW
                     GVGDPIPIYGPPDEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTF
                     GYLLETAHSRVAWLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTV
                     LALNQVIRSPRVILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(769..1098)
                     /gene="yjdP"
                     /locus_tag="OQN13_003822"
     CDS             complement(769..1098)
                     /gene="yjdP"
                     /locus_tag="OQN13_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCS1617039.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(1167..1484)
                     /locus_tag="OQN13_003823"
     CDS             complement(1167..1484)
                     /locus_tag="OQN13_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HCS1617040.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            1737..3722
                     /gene="yjcS"
                     /locus_tag="OQN13_003824"
     CDS             1737..3722
                     /gene="yjcS"
                     /locus_tag="OQN13_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCS1617041.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            3931..4206
                     /locus_tag="OQN13_003825"
     CDS             3931..4206
                     /locus_tag="OQN13_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617042.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         1074 a         1118 c         1022 g         1035 t
ORIGIN      
        1 taacgaggcg atcaccctga tcgacgccgg gctgcacgat ctcaccgatc gctggtctcc
       61 cggatcgttc cagcagtttt tgctgacgca ttatcatatg gatcacgtcc aggggctgtt
      121 tccgctgcgc tggggcgtgg gcgatccgat cccgatctac ggcccgccgg atgaacaggg
      181 ctgcgacgat ctgtttaaac atcccgggct gcttgatttc agccacacgg tggaaccgtt
      241 tgtggtgttt gatttgcagg ggttgcaggt cacgccgctg ccgcttaacc actcaaaact
      301 cactttcggc tatttactgg aaacggcgca cagccgggtg gcgtggctgt ctgacaccgc
      361 tggcttgccg gaaaaaacgc tgaaattttt acgcaataat cagccgcagg taatggtgat
      421 ggattgcagt cacccgccgc gcgcggatgc accgcgtaat cactgtgatt taaataccgt
      481 gcttgcgctg aatcaggtta tccgctcgcc acgggtgatt ctgacccata tcagccacca
      541 gtttgatgcg tggctgatgg aaaacgcact accgtcaggg tttgaggtgg ggtttgatgg
      601 gatggagatt ggggtggcgt gatgagaggg aatgtgcgcg ctggccccct caccctaacc
      661 ctctccccag aggggcgagg ggaccgattg tgctcaatat tgaatattgc gctcgttttc
      721 tccctctccc cattggggtg aggggcgatg cctgctccat acccaacctc atcgcccata
      781 ctcatcttcc attctccgct cttcatcctc cagttgccga cgctcctgat caagctggcg
      841 ctggcgatcg tccagctgcc tgcggcgatc ttcaaactgg cggcggcggt cgtcatattg
      901 tctgcgccga tcgtcgctca cttcacgctg ccagccgtcg tcgcgcgaat cttcatagtc
      961 tcgcccacgg tcagggttat aagcgtcatt aatcgcctgc tgaatattac caatagtatc
     1021 gtcgataata tcagcctggg ccggaacggt ggacagcgtg agcagggtga ataaaagaaa
     1081 tagcggaaag cgtttcatta gccaacctca aaaagaaact ctatccacat tagtcattac
     1141 tcatccatgc aagtagtgga tgaatctcaa tttctccgct gctctattgc cgtaatcgcc
     1201 tccacgcgtt gttgatgacg accgccttcg tactgtgcgc ccagccacgc atccacaatc
     1261 atttttgcca gttcgaggcc aaccactcgt gaaccaaaag ccagtgcaac ttcactggca
     1321 taatgcggat aatcagtgcg ctctgacgac ctggtgtctt tatcaatcac ctcactcatt
     1381 gaccatgaat aaaaggctgg cacagcaacg cagaatcctg cggtgccagc gacatgaccg
     1441 cccattgctc cggtacgttt tttaaaaaga aacaccggtt gcattgataa attgtgctca
     1501 gtatttgccg aagtacaata tatataataa atatataaaa cacctcatgt tatttacaga
     1561 gataataaat tcgcgtaatt ttccctcttc agcacaaatg cctaataaaa tggatcgttc
     1621 agctcacact tccacttatt aaagtcgcaa attcccaatt atgataaccc cagctttaaa
     1681 tgacgtattt tgcatggatt acatatttac gacataaatg ataaatggag atttacatga
     1741 ataactctcg gttattccgt ttgagcagaa ttgttattgc gttaactgcc gccagcggca
     1801 tgatggtaaa taccgctaac gcgaaagagg aagcgaaagc cgccactcaa tatacccaac
     1861 aggttaatca gaattacgcc aaatcattac cgtttagcga tcgtcaggat tttgacgacg
     1921 ctcagcgtgg atttatcgct ccgctgctgg atgaaggtat tctgcgcgat gctaatggca
     1981 aaccatatta tcgcggggaa gattataaat ttgatatcaa tgcgcccgcg cccgaaaccg
     2041 ttaaccctag cctgtggcgt cagtcgcagt taaacggtat ttccggcctg tttaaagtca
     2101 ccgacagaat gtaccaggtt cgcggtcagg atatctccaa catcaccttt atcgaaggtg
     2161 ataccgggat tattgtcatc gacccgctgg tgacgccacc gagcgcaaaa gccgcccttg
     2221 acctttactt ccaaaatcgc ccgcaaaaac cgattgttgc ggttatttat acccacagcc
     2281 ataccgacca ctatggcggc gtgaaaggca ttatctctga agctgatgtg aaatccggta
     2341 aagtgcaggt tatcgcccct gctggcttta tggacgaagc catcagcgaa aacgtactgg
     2401 cgggtaatat tatgagccgc cgtgcacttt actcctacgg cctgctgctg gcgcataacc
     2461 ctcagggtaa catcggcaat ggtcttggcg taacgctggc atcgggctac ccgagcatca
     2521 tcgcaccgaa caaaaccatc accaaaaccg gtgagaagat gattatcgac ggcctggagt
     2581 ttgacttcct gatgacccca ggtagcgaag caccagccga aatgcacttc tatattccgg
     2641 ccctgaaagc gctgtgtacc gccgagaacg ccacgcatac cctgcacaac ttctacactc
     2701 tgcgcggcgc gaaaacccgc gacaccagca agtggaccga gtatctgaac gaaacgctgg
     2761 atatgtgggg taacgacgcg gaagtcctgt ttatgccgca cacctggccg gtctggggca
     2821 ataagcatat caatgattat attggtaaat atcgcgatac tatcaagtac attcacgacc
     2881 agaccctgca cctggcgaac cagggctaca ccatgaatga aatcggcgac atgattaaac
     2941 tgccgcctgc acttgccaat aactgggcca gccgtggcta ttacggttct gtcagccaca
     3001 acgcccgcgc ggtgtataac ttctatcttg gctattacga cggtaacccg gctaacctgc
     3061 atccgtatgg tcaggtggag atgggtaaac gttacgtgca ggcgctgggc ggttctgccc
     3121 gtgtcatcaa cctggcgcaa gaagcgaaca agcaaggtga ttaccgctgg tcggcagaac
     3181 tgctgaaaca ggtgattgcc gccaacccgg gtgaccaggt cgcgaagaat ctgcaagcga
     3241 ataactttga acagctgggc tatcaggccg agtccgccac ctggcgcggt ttctacctga
     3301 ccggcgcgaa agagctgcgc gaaggggtgc ataagttcag ccacggcacc atcggttccc
     3361 cggacaccat tcgcgggatg tcggtcgaaa tgctgttcga ctttatgtcc gttcgcctcg
     3421 atagcgcgaa agccgcgggt aaaaatatca gcctgaactt caatatgagc aatggcgata
     3481 acctcaacct gacgctgaac gatagcgtgc ttaactaccg taaaacactg caatcccaag
     3541 ctgacgcctc tttctacatc agccgtgaag atctgcacgc cgtgctgacc gggcaagcca
     3601 aaatggcgga tctggtaaaa gcgaagaaag ccaaaattat tggcaatggc gcgaaactgg
     3661 aagaaattat cgcctgtctg gataatttcg atttgtgggt gaatatcgta accccaaatt
     3721 aatgtcactc gcttttgggg gcgctgaagc ccccaaaagc atttatttta aaaatatata
     3781 cagccaatta ataaataaaa tatataagtt atgaatgcgg tgactgaata aagctatttt
     3841 tattcatcga gaaaatagcc atactgacgc cttcaatgga tattgatatt cactacagat
     3901 attgttcgca gcagacagga tgtatttaat atgccaacag ttctctctcg catggcgatg
     3961 caactcaaaa aaacagcctg gataattccc gtcttcatgg tttcgggatg ctcattatct
     4021 ccggcaatcc cggtgatcgg tgcttattat cccagctggt ttttctgcgc cattgccagc
     4081 cttattttga tgctcatcac gaggcgagtt attcagcggg caaatatcaa tctggcattt
     4141 gtcggaatta tttataccgc cctttttgct ctctacgcca tgctgttctg gctggcattt
     4201 ttctaatcta tttgagacgc gctcatggaa agtacgccga aaaaagctc
//
LOCUS       DAJVRF010000218         4243 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJVRF010000218 DAJVRF010000000
VERSION     DAJVRF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.218"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(42..1406)
                     /locus_tag="OQN13_003826"
                     /pseudo
     CDS             complement(42..1406)
                     /locus_tag="OQN13_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
     gene            complement(1471..1590)
                     /locus_tag="OQN13_003827"
     CDS             complement(1471..1590)
                     /locus_tag="OQN13_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCS1617043.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1745..1963)
                     /locus_tag="OQN13_003828"
     CDS             complement(1745..1963)
                     /locus_tag="OQN13_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1617044.1"
                     /translation="MKKLLRTLSAVDARVVCKPHRLGRSMWHLVVLLEELCKRGINFR
                     ALAQSIFAQQWGDECCKSKRICDLKVIV"
     gene            complement(2317..3795)
                     /locus_tag="OQN13_003829"
     CDS             complement(2317..3795)
                     /locus_tag="OQN13_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HCS1617045.1"
                     /translation="MLVSKSNEFNTSSFIDSGNCNERKSSESMELLAYSIINLICKEA
                     ASGTYRGALETLQKMMSECIYHEGNAFVIMGSGEQLKRIKYDVDENNLKVFNVYFDNN
                     EELVTDGEPDVVCLSKQVWEDLLIKLKLENKENAVSETDLSSNKNNVDQFIECAKRNE
                     QTLFDNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQQV
                     KGHYGDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNG
                     NCSERLFNFYECCYKFLPRAQPQDKIESYNSALQAFSIFRSSTLNNNDVGFNFKLFPE
                     VKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGLDFKGCFFSGQNFSNY
                     DIQYVNWRTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGV
                     APFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT         1195 a          925 c          754 g         1369 t
ORIGIN      
        1 ataagtgata ctggttgtct ggagattcag ggggccagtc tagcaactaa agcactgccc
       61 gtctcctggt taaccccatg tgctataccg ccggtataga caatcagcga gcctccggcg
      121 ctaatattgc tgccagttgc cgtaccatct ttctcaacaa cctgccggct cccggtggaa
      181 atatttgtcg tgtcagcttt cccgccgctc ttgatatttt gtgttccgct gttgatattg
      241 gtacccgtgg caataccatg attattaata ttctgtgtac caccattgat tatggtattg
      301 gtcgcgtttc tggtaacatc cattaccccg ccgttatcta ttcgggtcgc atcagcttta
      361 gcattcgtta aaactgacat tgttccttta tctttaataa tcgtcttatt tgccgaacca
      421 tatgcgttta tgtctaaatg accaccgttt tccagcaaca cattgtctgc cacgtgattg
      481 tggatggaga atgcaccttc actattcgta ccgctcaccg tcgtaccgtt agtgtttgtt
      541 tttaaaattg caccatcgtg ctgggtaaca tttgttgccg taccaccact aacatcaagc
      601 acgccaccgg aataaacttc aataacatcc gaggagctgg tgtaatcaac aatttgcgta
      661 ccaccagaat agatctgagt attttttgcc gttgacttat tattcagaga ctgagttcca
      721 ccttcaatcg tcgtgtccag cgcatggctt tcatatactc tttgctcacc gccattttta
      781 atggtggttg ttcctactgt gctctgttca acatactgtc gtccaccatt tatgattgtg
      841 ttcgttgcca tacttccttg cgtgatgtcc tgataaccgg acttatttat cgttgtacca
      901 tcaacatgcc cctgagttgt tactgtctgc cctccaccat caatgatggt tccattcgca
      961 gtccccccac ttatgtgtga attaccaccc ttaattgtcg ttccattgct gataccgcct
     1021 gtataaacgt cctgattgcc actgtcgatt atcgtaccgg tggaaatgcc cccgtcatga
     1081 attgtctgtc ttcctccgtt aatggttgta ttattagact gccccatata attgttattc
     1141 cttccaacat cttgatatcc accagattca ataagacttc cattagatac tccgccataa
     1201 acactctgct ggccatggtt gataatatga gtgttatttg ttgtacctcg ttcatctact
     1261 cgttggttgc catctacagt ctcatcgttt accacaccaa taacattagt agtaaaggcc
     1321 gccatcacgg gcggggcata tatcaaggca gatatcaata aggaaagtac tgagcggcga
     1381 caataatggg gactggtcct gttcataaat ttcatcctct gaaaagtgaa tactgagtag
     1441 cgtttaagcg actttagctc tgctgcaaca tcagcccgca ggcaccagac cggggggttc
     1501 atcctgaaga gacagcgcaa gtgtattgtg ttcaccgctc atcaaagaca tcatgatgaa
     1561 atgatgatgt tccacataag aaagaggcat tttttaaaca cagtgcgctg aagtgtgatt
     1621 ggataaaaaa gtcaatctat tcaggaaata cgggcgtatt cttttctttc gacaatcagg
     1681 ccgtcggcaa aataaaatga tttacataat cgtttctgat gaatatcttc tgctcacata
     1741 aaaatcacac aataactttg agatcgcaga ttcttttact tttacagcat tcgtcccccc
     1801 attgttgggc aaatatagat tgggccagag cacgaaagtt aataccacgt ttgcacagct
     1861 cctccaacag cacgacaaga tgccacatac tgcgccccag tcggtgcggt ttacagacta
     1921 cccgtgcgtc caccgccgat aatgtcctga gcagtttttt cagtccggac ctcctgccac
     1981 ggttttacgt tgaagaggta accctgagca cgcagttctt cagtcaggcg tggtgcaccg
     2041 taacgctgtt attgctgggt aagatcaaaa aactttcagg cagctaagga aagttgaacc
     2101 agacattaga tgaaatattt caaccaatta cagcaccaat ttaaactcag taaatcacag
     2161 cacagatcaa ggggttacgt gaaagcgtag cccctttttc tttggtagtg gcagcaaaat
     2221 ggttgtagtg taaaaaataa tcccgtttaa tcaatcaata atacatattg tttcaatcta
     2281 cgttattatc tctttgtaaa aatagccatt tattaatcat tgaaaactgc ttttagaact
     2341 tgatacaacg ggactagtca caacaggact attctcaacg ggatcatcct cagaggaact
     2401 atcatcaaag tcatcatcca taaataaaat atcatcgaat ggtgccacgc ccgtgatgag
     2461 ttttatttta ttattacgat cagtcaagac tccacttaaa ccgttttcgc tcacaggttt
     2521 taatgatttt tcattactct tgttgtaagc aggcgcatta aaaatacacg gagtatcaag
     2581 atcaaacaat gacgttctcc agttcacata ttgaatatca tagttactga agttctgtcc
     2641 agaaaagaag catcccttaa aatccaatcc acctaaatta tataaaccat cctcttcttt
     2701 ttggagagta atgttaattc tggctatctc ccggacaaaa cttccttttt tgtatttgaa
     2761 taccgtttca agattttcac cagacagctt gacttctggg aataatttaa aattaaaccc
     2821 tacatcatta ttattcaaag tagatgaacg aaagatggaa aaagcttgta gtgctgaatt
     2881 atagctttcg attttatctt gaggctgcgc tcttggtaaa aacttatagc agcactcata
     2941 aaaattgaat aaacgttctg aacaattccc atttttatca attaataccc cccggaattc
     3001 tttcacaaag gcatccgtta ttttttgaat atctatggtg tgttcctttg actctgacaa
     3061 agatggagtc tgttgaagtg cattgctcat ctggttaagg aacatctgca tttctttaaa
     3121 tttatctcca tagtggcctt taacttgctg agagttttta gttacaggtg aaaccttatc
     3181 tatttggttg tcatataaat tatgtggttc catacagaca tttggttgtg tttctaaaat
     3241 aacactacgt gtactacctg gcttaaatga atcaacatga aaatcacttt ttcttatatt
     3301 atcgaatagg gtctgttcat ttctcttagc gcattcaata aactgatcaa cattattttt
     3361 attcgatgac aggtcagttt cagaaaccgc attttctttg ttctccagct ttagtttaat
     3421 gagaagatct tcccagacct gcttgcttaa acatactacg tcaggctcac catcagtaac
     3481 taactcttca ttattatcaa agtatacgtt gaatactttt aagttatttt catcaacatc
     3541 atatttaata cgttttaatt gctctccaga tcccataatg acaaaggcgt tgccttcatg
     3601 atatatacat tcagacatca ttttttgtaa agtttcaaga gcaccgcgat acgtccctga
     3661 ggcagcttcc ttacaaatta aatttataat actataagct agtagctcca tggattcaga
     3721 agattttctt tcattacaat ttccactatc tataaatgag ctagtgttaa attcgttgct
     3781 tttactaact aacattgtct attcctcagt taatgtctac atggctattt ttaattttat
     3841 tattgtttgt cactataaaa aatcgctcat ttgagacaat tgctggcatt aacagcttca
     3901 tatgctatac atggtacttt taattaaatt agcacaagaa tgttaaattt aataaacaaa
     3961 aagttatttc gctgtatgat aaaaaccacc cgttataatt tattagtgaa attcgttttt
     4021 cgagtgttag aaatttatat ctaaatagcg ttgattaatg agcaaagcca gactcctgta
     4081 acgctcacaa gactcatcta cctgcgacgg gtattctggt cagtagtaga tgtttaaggc
     4141 gtggcagaga cattttatcc ttactctacg gcattggccc gcatacaaat cgtggttgtg
     4201 acactcactt atcatcagtg agcgaacaga gaatagttca gtg
//
LOCUS       DAJVRF010000219         4206 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJVRF010000219 DAJVRF010000000
VERSION     DAJVRF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.219"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..793)
                     /locus_tag="OQN13_003830"
     CDS             complement(<1..793)
                     /locus_tag="OQN13_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCS1617046.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLL"
     gene            complement(790..2187)
                     /locus_tag="OQN13_003831"
     CDS             complement(790..2187)
                     /locus_tag="OQN13_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCS1617047.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2342..3340
                     /locus_tag="OQN13_003832"
     CDS             2342..3340
                     /locus_tag="OQN13_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1617048.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     ELIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3451..>4206)
                     /gene="ompG"
                     /locus_tag="OQN13_003833"
     CDS             complement(3451..>4206)
                     /gene="ompG"
                     /locus_tag="OQN13_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCS1617049.1"
                     /translation="AEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEV
                     HYQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGW
                     LSMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFS
                     TQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNG
                     LSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1034 a         1147 c         1029 g          996 t
ORIGIN      
        1 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
       61 gcgtggcgat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
      121 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
      181 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
      241 ggtcgttttg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
      301 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg gcctttgccc gtgccgtggc
      361 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag caccataagc
      421 actgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
      481 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtacaccct
      541 gcccgacaac acttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
      601 tgcgtttcgg acgtaacgct gcatccatta ccgcttcaac ttgcccctct tcttcaggcg
      661 cttcatcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
      721 ccctgaattt tggattctga tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
      781 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
      841 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
      901 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
      961 gagaggcgca ccatcgctga gccgattacc gcgcaatgcc ggaattttct cgccgttgac
     1021 atcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     1081 cccttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     1141 attggcgtgc tgatcgaccg ttacatggtc cgctttcgtt gcggcaaaca gcagtttgtc
     1201 gataaccggc gagaataagc ggcgaaagag cgtgcgctgc ccgtagtgga aactttgcat
     1261 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     1321 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     1381 cttcaccacc ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     1441 cttatcggcc tgcgccagtt tggactcgcc ccaggtatcg acatccggcc acgggaagaa
     1501 ttgcagcgcg ggcgcacctg ccatatctcc tggcaagaca aagcgccctg gctgaataaa
     1561 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacgcggcgg caatgtctgc
     1621 cagccggttt tcgtcggcag gtgctagcgg gtccagccct tcgcacatca ttcgccattt
     1681 gaccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgtg accagcttaa
     1741 atagtcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatctc
     1801 cagatacagc gtggaggtgt ctttgaagtg gcgtagcagc gaatcgttcg atttaaaacg
     1861 tagcgccagg cggatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcgcc
     1921 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgagg
     1981 aatgcgtttt acgcccagca ggcgctcttc acgcaccgcg cttaacagcg gcaagcgtgc
     2041 tccggcgtga atattgagca actgattgac catcgcagtg ataaacgctg ttttgccgct
     2101 gcggcttagt ccggttacgg cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     2161 attaagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     2221 aatcattcgt aaggatatga atgtgggggc caggccattg attatcaact gcccgtgttg
     2281 tcatcgacgg gcgaactcca taagatgtgc agcattcacg ttcggacgga gttcctgacc
     2341 tatgtcccct actatttatg atattgccag ggttgcaggc gtatcaaaat ccaccgtatc
     2401 acgcgtgctg aataagcaaa ctaatatctc cccggaagcg cgcgaaaaag tgttacgggc
     2461 cattgaagaa ttacagtatc agccaaacaa gctagcccgc gcgctgacct cttcgggttt
     2521 tgatgccatt atggtgattt ctacccgttc gaccaaaact acggcgggta atccgttttt
     2581 ctctgaagtt ttgcatgcca tcaccgccaa agctgaagaa gaaggtttcg acgtgatatt
     2641 gcagacgtcg cacaacccgg cagaagactt acaaaaatgt gaaagcaaaa ttaagcagaa
     2701 aatgattaaa ggcattatta tgctaagttc gccagcggat gagtcatttt ttgcccaact
     2761 cgataaatac gatattccgg tggtggtgat tggcaaagtt gaaggtcaat atgcccatgt
     2821 ttattctgtc gataccgata attttggcga cagcattgcg ttgaccgatg cattaattga
     2881 aagtgggcat caaaatattg cctgcctgca tgctccgctt gatgtccatg tttcagtgga
     2941 tcgggtaaat ggttataagc agagcctggc tgcgcataat attgcagtgc gtgatgaatt
     3001 gattgttgac ggtggttata cccatgaaac agcactgcaa gccgcgagag aattattaag
     3061 ccagtcaccg ttgcctgagg cggtgtttgc cactgacagt ctgaaattaa tgagtattta
     3121 tcgtgcggca gcagagaaaa atattgctat tccgcagcag ttagcggtgg tgggttatag
     3181 taatgaaacg ctgtcgttta tattaacgcc tgcaccgggc ggcatcgatg ttccgacgca
     3241 ggagttaggt caacaaagct gcgagttatt attccgctta atttctggaa aaccgtcacc
     3301 acagaatatt accgttgcca cgcatatgac gctgaaataa ataccagcta ccttgcctga
     3361 atgccggatg cgatgctagc gcatcttatc cggcctacat ttatagatcg gataaagtac
     3421 tcgcgccgca tccggcaatt tagcccatta tcagaacgag taatttacgc cgacacctgc
     3481 ataatggaat ttatcactgt ctccttcgtc gtgatcctgc cactcaaacg cgtattccag
     3541 cgaaacggaa agtccgttct ggaaatcata accgtaaaat aaacctacac ggttaaaatc
     3601 atggccttca cgttcaatat catcctgcca gtcccagtta ctccagcgat ccagcccaat
     3661 gcgcgtatac ggcgtcaccg agtggttgcc gagcgtcagc ggcaaatagg cgcgaatttc
     3721 ttgcgtggaa aactcaccgt tattgcggct gtcgtccata ttgaagccgc gctcgagata
     3781 atagttcact cgcaaggcaa ccgtttcgtt gaaggtatat tgcagacctg tttccgtttc
     3841 gacacgggta tcagcgtaac cggtagtgtt cagatcgttg gcaaatttat acatcgacaa
     3901 ccaaccgttg aaacgtaaat cgtcagtcag tttcacatcc cagtctggcg cgattttcca
     3961 gcgctgcata ttcgccgtgt ctttacccgg ttcatcaacg tagtgataac cataattacg
     4021 gaaaccgccg gtcaggccga aactgaaatc atcgttttcg aggaactgat aatgcacctc
     4081 cagctccggg cgatcaaacc acgttccacg tttacccgcg ctataatcta ccggcccttc
     4141 ctgataatag gccagagcaa ttctccacgg cccgttggcg gcattaaaat agactgaagg
     4201 ctccgc
//
LOCUS       DAJVRF010000220         4076 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJVRF010000220 DAJVRF010000000
VERSION     DAJVRF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.220"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..784
                     /locus_tag="OQN13_003834"
     CDS             <1..784
                     /locus_tag="OQN13_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS1617050.1"
                     /translation="AKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVN
                     QQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQLS
                     DTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQG
                     NRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGS
                     RLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            797..1757
                     /locus_tag="OQN13_003835"
                     /pseudo
     CDS             797..1757
                     /locus_tag="OQN13_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(1795..2694)
                     /gene="nhaR"
                     /locus_tag="OQN13_003836"
     CDS             complement(1795..2694)
                     /gene="nhaR"
                     /locus_tag="OQN13_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCS1617051.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2760..3926)
                     /gene="nhaA"
                     /locus_tag="OQN13_003837"
     CDS             complement(2760..3926)
                     /gene="nhaA"
                     /locus_tag="OQN13_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCS1617052.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1225 a          953 c          861 g         1037 t
ORIGIN      
        1 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
       61 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
      121 tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg
      181 gagaactccc atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag
      241 tggtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca
      301 gttatccgat acatttgcaa ttctggatgc gcctgggtta gaaggtgcac atattaatgg
      361 acaaaaatac aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca
      421 tcgtgaaaac catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg
      481 caatcgtcaa attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga
      541 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg
      601 ctatgacgta ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct
      661 ttttattcgc gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa
      721 cttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
      781 gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat attcttttat
      841 tgtgcaaagt gctccctgtg cattgaccaa tgtcggggaa caacgtggaa catatatact
      901 caagccactg agtatgaaag gtaatctcac cgccgaaaaa attgaaaaca ccggaaatat
      961 cattgagtgt gcagacttac aagagacgac caacgccatt aaagttcatt ttttaacaca
     1021 gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaatc
     1081 tgacggtaaa aaaaatagag cttccaggaa ggtcatctta taatttagaa gaaatgctat
     1141 ctgcgataaa tttcaccatt ccatactcta tcgcatctgt atcagatagt tccaacaccg
     1201 cagatatttt tccaggaact ccttttcctt tgttgttcga aattgaagtg agttcatgta
     1261 cagggaataa tactaattgt aatagcgtcc gctttaatta taatttaaat actattttac
     1321 aggtaaattt aatgacctgt ggttttgaag atcaatccat cgacactgga cgatttgtac
     1381 tgtctcgtat caataatgaa aattatcagt ttcatcatat aagatttgac tgcatccagg
     1441 gcgagcaagg tgaattagtt tttgaaccca gtgatgttga atattacttt gaaccagtga
     1501 cgatacagga gtcaggaacc agcatattaa aaaatgagtt agaaaacagt gaggacggtg
     1561 cgggtcaagt aggctttcaa ccctccaatg atggtacgaa tgaaatccag tatggtaaaa
     1621 gcaattacta ctcttttcag catccccatg aaggaagtaa tcagattcct ctatttattc
     1681 gcccgcgtac atatggaaat aatgtctcaa gcgggcaaat aatgagccga gtcaaaatcg
     1741 tggttatgta caactaatct atctccccca ccttaacttt gggggaggtg ccgattaacg
     1801 cgccgctgga ctaaaaagcg cagaataatc cgtattgcag attcgctgta ccgccgggtg
     1861 ctgaatcatc cgctcagcaa aaatagcatg gtactcttcc atcacattct cgacgcgacc
     1921 aatttctacg acagttttat cggcataaaa gtcatatgca taaagcgttg gggcaacgaa
     1981 gattgcattg tgcatcgcac caaaagcttt catcaacgcg gcatcatcaa actcgccgag
     2041 gatttctacg tttaatccct gggagttaaa ccagttaagc aatttgcgcc ctaacattga
     2101 acgtcgccca ggaatcaaaa gtcgccgttc ttccagacaa gccgggaacg atttttctgg
     2161 tggtggattt gtacaccaga aactcacgcc acattcgcca attctcacgg agaacaggcc
     2221 ttcctgctgc gtagagtcta tcggacagtc agaaatgatc atatccagtt tatgctgact
     2281 taattgctcc agcagcattt cgtgggtgga ttcgaagcag cgaagatgaa tgggctcgcc
     2341 ttctaccact gcggcgttaa gtacgctact gaccaggcgt ttggaaagtg catcagccac
     2401 gccaacgtca aacaataaat tggattcttt gcgatagttc acaatatcca gcatttcctg
     2461 gcttaaggtg aacattttat cggcatagcg atagaccagt tctcccagct cgctgggttc
     2521 gagaccacgt cccttgcgtt taaataattt gccttgcagg cgctcttcca gcgctcgaat
     2581 ctgtccggta atggtttgtg gagttaaata aagcgcctcc gctgcgccaa ccacggaacc
     2641 ttctttatag acatgccaga agtaatacaa gtggttgtaa ttgatatgag acatgctcat
     2701 ttctctccct gataacaatg aaaagggagc cgtttatggc tccccggtaa accgtcctgt
     2761 caaactgatg gacgcaaacg aacgcgtaac cagctgtatc caattaccgc cgaagagata
     2821 gaaccgacca ggataccgag tttcgcccag ttaatcagtt ctggatctac gctaccaaag
     2881 gccaggctgg caataaagat agacatagta aaaccgatac cgcacaggat ccccaccgcc
     2941 ataatttgct gataagtcgt tccctcaggc agatgcgcca gtttcaaacg cagcgccaac
     3001 cagcagaaca gactaatccc cagcggtttg ccaatcagca agccagcgat gatccccaat
     3061 ggcagaatgg aggtcaagcc atccagcgtg acgccttgca gtgaaacgcc agcattagca
     3121 aatgcaaaca gcggtaaaat cagatacgcc acccacgggt gcaatacatg ctccagtcgc
     3181 ttcgctggag aacgcccatg cttctctttc aaaggaataa agaagccgac aattaccccc
     3241 gccagggttg cgtgaacccc cgatttcaac accgcagtcc acaacaccac gccaacaaga
     3301 atatagacgc ccgtgcggcg tacaccacac agattcaata ccgcgagtac cgcaattgct
     3361 acagccgcga cgccaagaga ggccatcgat aagtcattag tgtagaacaa tgcgatgata
     3421 atgatggccc caagatcgtc gataatagcc agagccatca aaaagatctt cagtgctaac
     3481 ggaacacgac ttcccaacag cgccagcaca ccaagtgcaa aggcaatgtc agtagccgcc
     3541 gggatcgccc acccttcgcg ggtaatcgga tcggcatagt taaaggccag atagagtaat
     3601 gccggaacaa tcatcccacc aatagcggcg ataaccggaa atgcggcctg gcgtaagctg
     3661 gctagcgatc cttgcatcag ttcacgttta acttccagac cgactaacag gaaaaatacc
     3721 gccatcagcg cgtcatttat ccataacagc atgtttttgt tgatttcgag tgaaccaacc
     3781 cggagctgaa ccggcgtctc cagaaagtcg tgataccatc cactggttgc gccgctgttg
     3841 gccatcatca tcgccaggat agcggcaatg ataagaataa tgcctcccga ggcatcactg
     3901 ctaaagaatc gatgcagatg tttcactttt tatttctctt tcaggtgaat agatcgtttt
     3961 tactatttta ccccgcacaa atcatcgtta aaatcagatt atagtgtagg aataactcgt
     4021 tttttacgag caattaaagg atttgcgcca tcagcggtga gatgagtttt tgacgg
//
LOCUS       DAJVRF010000221         4073 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJVRF010000221 DAJVRF010000000
VERSION     DAJVRF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.221"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..1629
                     /locus_tag="OQN13_003838"
     CDS             <1..1629
                     /locus_tag="OQN13_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCS1617053.1"
                     /translation="FKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQ
                     TDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDT
                     LNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAA
                     ANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGD
                     TPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASELDC
                     WDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKI
                     LDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVS
                     ELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAE
                     EYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDA
                     PVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(1863..3146)
                     /locus_tag="OQN13_003839"
     CDS             complement(1863..3146)
                     /locus_tag="OQN13_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCS1617054.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3281..3973)
                     /locus_tag="OQN13_003840"
     CDS             complement(3281..3973)
                     /locus_tag="OQN13_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1617055.1"
                     /translation="MLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTKPVAATRA
                     AKSEVRRSRLTADEYLKIYQTAESSPCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGY
                     LYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLSSGTVS
                     RYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRD
                     DRGREWDKIEIK"
BASE COUNT          976 a         1018 c         1045 g         1034 t
ORIGIN      
        1 ttcaaaaacg gttacgtcaa aaacaaagtc atggagttcg ttggaccggg cgttagcgcg
       61 ctctctaccg atttccgtaa cagcgttgac gtgatgacca cggaaaccac ctgtttaagc
      121 tccgtctggc aaactgatga agaagtgcat aactggctgg cgctgcacgg tcgcggccag
      181 gattactgcc agcttaaccc tcaaccgatg gcgtactacg atggctgcat cagcgttgat
      241 ttaagcgcca tcaaaccaat gattgcgctg ccgttccacc cgagcaacgt gtatgaaatc
      301 gacacactga accagaacct gaccgacatt ctgcgtgaga ttgaaattga gtccgaacgc
      361 gtggcgcacg gtaaagccaa actctcgctg ctggataaag tggaaaatgg tcgcctgaaa
      421 gtgcagcagg ggattatcgc gggctgttct ggcggtaact acgaaaacgt catcgcggcg
      481 gcgaatgcac tgcgcggtca atcctgtggc aatgacacct tctcgctggc agtttacccg
      541 tcatcacagc cggtgtttat ggatctcgcc aaaaaaggtg tggtagcaga tttgattggc
      601 gcaggcgcaa tcatcagaac cgcgttctgc ggcccatgct ttggcgcggg cgatacgcca
      661 atcaacaacg gtttgagtat tcgccacacc acgcgtaact tcccgaaccg cgaaggctct
      721 aagccagcta atgggcagat gtcagcggtg gcgttgatgg acgctcgttc tatcgctgcg
      781 actgcggcaa acggtggcta tttaacctct gccagcgaac ttgattgctg ggacaacgtg
      841 ccggagtacg ccttcgatgt aacgccgtat aaaaaccgtg tttatcaggg ctttgtgaaa
      901 ggggcaactc agcaaccgct gatttacggg ccgaacatta aagactggcc ggaattgggt
      961 gcgctgactg acaatatcgt cctgaaagtg tgctcgaaga tcctcgacga agtgaccacc
     1021 accgacgaac tgattccttc cggtgaaacc tcttcttatc gttcaaatcc gattggtctg
     1081 gcggagttta ccctgtctcg ccgtgatccc ggttatgttg gcagaagtaa agcgactgct
     1141 gagctggaaa atcagcgtct ggcggggaat gtcagcgagc tgacagaggt gtttgcgcgc
     1201 attaagcaga ttgctggtca ggagcatatt gatccgctgc aaactgaaat tggcagcatg
     1261 gtctatgcgg tgaaaccagg cgatggttct gcgcgtgaac aggcggcgag ctgccagcgt
     1321 gtgattggcg gtctggcgaa tattgccgag gagtacgcga ctaaacgcta ccgttctaac
     1381 gtcatcaact gggggatgtt acctctgcag atggcggaag tgccaacctt tgaagtgggg
     1441 gattacattt acatccctgg cattaaagcg gcgctggata atccgggtac gacgtttaaa
     1501 ggttatgtga tccatgaaga tgcgccggta acggaaatta cgctctatat ggaaagtctg
     1561 actgctgaag agcgcgagat tatcaaggcg ggtagtttga ttaacttcaa taaaaaccgt
     1621 cagatgtaaa aagcgccatg tgaatgtagg tcgcattcgg cacttattgt cggatgcgat
     1681 gcttgcgcat cttatccgac ctacgaatcg catcgaatct gtaggccaga taaggcattt
     1741 acgcagcatc cggcacttat tgtcggatgc gatgcttgcg catcttatcc gacctacaaa
     1801 tcgcatcgaa ccgtaggccg gataaggcgt ttacgccgca tccggcaaat agttaattgc
     1861 tcttacttct tcgcctctgc aaccacttta ctacccacgc cgcggttatt gtattcccac
     1921 atgcggttgt agttagtgtc attcagattg cgctgtattt cgtcgttatc atctacgctg
     1981 ccggtattac ccgcaaacgg acgattagag atcaccgcat cggcccacgg tttagccgtg
     2041 ttaaaacctt cgttgatggc gctatcacgg atcaccacct gaccgttggt attggcatca
     2101 acatccagcg agcggcccag ttgcgccaca ccatcaccga aagcattgaa acggctgttt
     2161 acggcgagga aaccgtagta catgttggac agcgtagccg gtgcaaacac atacgcttct
     2221 tgctgagtac gtgagttcac cacgcggaat tcggtgttat cgaacaccac tgcgccgcga
     2281 ccagaaacga tatccacatc cccttcaatg tagctgttgg tcaccagcgt acgcggctga
     2341 cgattcgttt ccagacggtt ctgcacaccg ctgttggtga caaagaaggt gttctgacga
     2401 ccgagaatgt taacgttgtt aatctgtacc tggtcaccat cagtacgcag tgccaccgcc
     2461 ggatggttac ctgcatctac gctatcgccc agcgtgtttt cgatggtcag attttgcagt
     2521 tgcaggccat tgttttgtga ccagaagacc gcagagcaga gaacaccgat actgtcgctg
     2581 cgtttgctct ggcagctatc gtacatatac cacgctggtt tacctggcat atatttgccg
     2641 cgcgggttga cgtcgtgacg ccagtcggca gggctcatgc caccatcaag ggaaagccca
     2701 atcttcacat caatcggttt ttcacctgta ccgtacagag taattccacc cggagcggca
     2761 gggacatata ccgttccctg atactcacca ggcatcacgg caatatactg gcgcttgttg
     2821 gtacgcttga taattgccgc atctaccgcc gcctgaatcg tggtatgcgt tacaccttga
     2881 gtgcccgccg ggccgacaac aaagtcaggt tgcgcaggca gggtaatcgg ggaaggattc
     2941 cacgctgcag cacctggtgt cagggatgca aaatagtgtt gagcatcgaa attctgcgct
     3001 tcttttgccg acagaatcgg gcgagaagag gtaccaggcg cggtttgatc agaaggacgt
     3061 tgatcgggcg gggttgagct acaggcggtc agcgtcacgc caaaagccaa tgccagcgcc
     3121 agacgggaaa ctgaaaatgt gttcacaggt tgctccgggc tatgaaatag aaaaatgaat
     3181 ccgttgaagc ctgctttttt atactaagtt ggcattataa aaaagcatcg cttatcaatt
     3241 tgttgcaacg aacaggtcat tatcagtcaa aataaaatca ttatttgatt tcaattttgt
     3301 cccactccct gcctctgtca tcacgatact gtgatgccat ggtgtccgac ttgtgcccga
     3361 gaagatgttg agcaaactta tcgcttatct gcttctcata gagtcttgca gacaaactgc
     3421 gcaactcgtg aaaggtaggt ggatcccctt cgaaggaaag acctgatgct tttcgtgcgc
     3481 gcataaaata ccttgatact gtgccggatg aaagcggttc acgacgagta gatgcaatta
     3541 tggtttctcc gccaagaatc tctttgcatt tatcaagtgt ttccttcatt gatatcccga
     3601 gagcatcaac atgcaatgtt gtagggatgg caatttttac gcctgttttg ctttgctcga
     3661 cataaagata tccatctacg atatcagacc acttcatttc gcataaatca ccaactcgtt
     3721 gcccggtaac aacagccagt tccattgcaa gtctgagcca acatggtgat gattctgctg
     3781 tttgataaat tttcaggtat tcgtcagccg taagtcttga tctccttacc tctgattttg
     3841 ctgcgcgagt ggcagcgacc ggttttgttg ttatatggcc ttcagctatt gcctctcgga
     3901 atgcatcgct cagtgttgat ctgattaact tggctgatgc cgccttgccc tcgtctatgt
     3961 atccattgag cattgccgca atttcttttg tggtgatgtc ttcaagtgga gcatcaggca
     4021 gccccctcct tattgcttta attttgctca tgtaatttat gagtgtcttc tgc
//
LOCUS       DAJVRF010000222         4034 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJVRF010000222 DAJVRF010000000
VERSION     DAJVRF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.222"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..1745)
                     /locus_tag="OQN13_003841"
     CDS             complement(<1..1745)
                     /locus_tag="OQN13_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1617056.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGY"
     gene            complement(1830..2165)
                     /locus_tag="OQN13_003842"
     CDS             complement(1830..2165)
                     /locus_tag="OQN13_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCS1617057.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2165..2521)
                     /locus_tag="OQN13_003843"
     CDS             complement(2165..2521)
                     /locus_tag="OQN13_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCS1617058.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2521..4017)
                     /locus_tag="OQN13_003844"
     CDS             complement(2521..4017)
                     /locus_tag="OQN13_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCS1617059.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHGYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT          910 a         1233 c          893 g          998 t
ORIGIN      
        1 aaatacccat caccacgtgg catctgccat ccggtcatgt catagacaaa atcatggaac
       61 tgtttgcccc actcgtcgaa cttatcattc agaccaaatc cactgttcag tgcatcagcc
      121 acaaaatttt taacaagcca gggatgtgct ttttcaaact cctgcgccat catgcccagt
      181 tcaagcaatc caccaatcag actgatcttt ccgaggccct ttaatttcag ccctgttttt
      241 cctgccgccg ttgtccattg attctgggcg actgttgccg cacgaatact tgccaccgcc
      301 ttcacaccaa tatacagggc agaaaccgtt gctattgttt ttactgcctg ttcccatccc
      361 cccattgctc tgacaacctg atcaacctcc tgccagactt ttttcacaac cggaccgacc
      421 gtttcccagt tatcaattat gagatatgca ccaccgacga gaagcgcaat taatcccttt
      481 gccggcgtca tattcatcac gctacccatg atttttgtta tgcgtgtcaa tgtgccaatc
      541 gccacgccca tcgtcagtaa tgccgcacca gttttcgcga tggtttttac gacctcagga
      601 ttttctctga caaacgtgcg cacttcttcc agaaaaggct tcatctcttt tatgccttca
      661 ttgagtgaag gcaggaaggt ttcccccagc gtggaagaaa tcgcattgat ctggttctgc
      721 agcagcaaca actgattttc tgtcgtcgcg gcacgggcgg catattcttt ctgcattgag
      781 ccgccatact gctgcgcatc cgccacacga ttaaagttgg ttcgcaacaa atccagattc
      841 gtgagcagcg gcgcaatggc ccccagggat tccttcccga acagcgcttt cagcacagca
      901 gcctgttttt ctttcggcac ttttgccaga gaatccagta cgtgcagcat ggccccgcgg
      961 gcgtctttct gcatatccgc cgccagtttt ttcgggctaa tgcgcaacga gcggagcact
     1021 tttttctgcg atttggtggc agaatccctc gctgtcagcg acaacataaa attctttatc
     1081 cccgtcgcag caatttctga ctccaccccc attccggcaa tggttgcccc cattgcggca
     1141 atctctccgg aggccacacc cgcaacactg cctaaagggc caatacgggt cacaacatca
     1201 gaaatctttt tcgcacttgc aggaccggta ttaccaagat agttaatctt atccgcaagc
     1261 cctgccacct ctccctgtgt cagtttaaag gcagtgcgcc actgcgccat catctgaccg
     1321 gattcttctg ccgtggtgtc aaaggccacg cccatcttca cggcatcgtc agtaaactgc
     1381 atcagctcgt cacgggcgat accagcctga ccacccgccg ccacaatttc ggcgatacct
     1441 tccgccgaca tgggcagttc tgttgacagg tcgcgcacct gctccgtcat tgccttaaac
     1501 gcttccggcg tatccagacc atccaccact ttccggacat ccgccatttt tgattcaagg
     1561 gcaatggcag atttgaccgg gagcgccagc gcccccagta ctgcggttcc ggcacccgct
     1621 gcgcccagta acaggctggc aaactccttt ttaaaacctt tcagctggcg ctgcattccc
     1681 ttcagcggtg ccgatacctt atccacggca gtgatgatcg ccttcagctg aaaactgtca
     1741 gccatgcttc atctcctcgt taatgcggac ggcctcggct tccagttctg taaactgcga
     1801 aacagccccc cgtcgcagct ccagtggatt cagtttccag aaccaggcaa cattgtagag
     1861 tcgtttccgg aggtgcttcc cgtctccgac tgggtaaaaa aacgcaggat ctgcatgctg
     1921 gtcttaaaaa tatccagttt tgccatctgc gccgccgatg aacgcgggat ccccgccagc
     1981 aacgggatat atttcagtgc cacctggctg tccagtttaa tactgccttc accggaaata
     2041 atgaaaggaa aacccagcgc ctcgatttcg tcatacgtgg gctcgcgcaa ctccagcaca
     2101 tgcagcattt cgttatgcgc tgtcaccggt ttttttaaaa ctatttctgt aacgttcatt
     2161 gccattactg ataccctccc tcttcgccgt ggaattcaag atctgccgtg ccttcttcgg
     2221 cattatgatt agcctctccg tgcagccatg ccgccgaaag cacatacacc atgccgtttg
     2281 ccagctcagc ggtaatcgtc atctggtctg aggtggtaat tttgtcgacc ggaaagtttt
     2341 tgggcacttt aaacgtgccc ttaacgtaag gtgaccgcca ggtctccttg taatccacat
     2401 cccctgccat gccgacgatg tcatcccgga cattggtgtt catcggcact tcaatgccac
     2461 ctgtcagcga taactgctga ccatccactt taaaaaaaca ggttccggcg atctttgcca
     2521 ttatgctgac tcctctgaat actgaagacg gaactggtta accaccgcaa agacacgtaa
     2581 ctgattaaca taatccggcg ggaacagcgt attcagtcgg ttcgggttat ctgcatcacg
     2641 ctcaactatc agatactgtt taaacagatc gtaattttcc acaataccgg cacgctccat
     2701 ctgacgatat gtcgccagaa gttccccttt gataacggca ggagtgacaa tcgcctgccc
     2761 cggaccaaaa cgggtaccat catttgccag cttgtgacgt ccgtacttgc ttgtgatgac
     2821 cgatttcagt ttgcgaagaa catatgcact ggtatgcaga gtttcactgt ccagatagct
     2881 gttgtctgcc acgccatacg cattcttttt gtaggtggtt acggaacgct ggatctgcaa
     2941 cgtgccgcct tccacataag ccgtcgccac gccgtgagat aaaagggtct gctgctctgt
     3001 catgatgaat cgcttacctt tcggtgccgg aagcatcccc accagctccc ccgtctgtgt
     3061 cggacgggca ggatcattcc ggataaatac cgcttcacgg gcaaggcgac tggcaaccag
     3121 ttcatcgaca ggcgactggg tttctttttc gtaaccggca agcgtgatat gttgctgatt
     3181 atgcatatct ccggcaccaa ccagctctga cagcgttccc agttttgcgg tatagccatg
     3241 cccgtataac tggcgcgcat aactccagcg accgctgctg tcattcattt cggtcatcat
     3301 catattgatg gaggcggcat cgttgaacgg cagaccgata aagtcgaatg cctcatcgcc
     3361 catagcagca acagcggcgg taagatcagg cgctccgcta ccggcagcac cgacttccgt
     3421 caccacccga agccctgctg gcagaatctc accaccacca gaaccataat aattcagaca
     3481 gaccggtaac tcattaccat acagcccctt atgacgggca gtcagcgtca ccacacctgc
     3541 atcagatgac gccgtaaacg gcagggtgat aaccccgttt accgcttcct taatcgcggt
     3601 ggcaaccgca gtggcatcat cgccattcac cacaggcacc tgtacacggg agcgcccgac
     3661 atacagactc agggtgccgc tttcctctgc ttctccggca accgtcaccc tgaccgtcgc
     3721 cgccgcccct ctggcttccg gtactgcaat aacatacagt tcaccgaaag gatctgtctg
     3781 acggtagact tccaccatac gcgccagctg gctccccgcc ccacaaatct ggcgggcata
     3841 atctgccgat ggcatcagca ccaggctgtt aacctcaatg gctgcatcgt tgctggcatg
     3901 cccgatcagt aatgcaggcg cgctggtcac cgctgtattt gccgcagaat tgttcatctc
     3961 ggcgtagaac aacggcacca gcgtattcga cggaacagta ttaaaactta tcgtcatgat
     4021 tttttagcct tatc
//
LOCUS       DAJVRF010000223         3978 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJVRF010000223 DAJVRF010000000
VERSION     DAJVRF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.224"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            171..506
                     /locus_tag="OQN13_003845"
     CDS             171..506
                     /locus_tag="OQN13_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1617060.1"
                     /translation="MLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTED
                     TIDGLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLT
                     LSYREVNRD"
     gene            499..981
                     /locus_tag="OQN13_003846"
     CDS             499..981
                     /locus_tag="OQN13_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617061.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1015..1095)
                     /locus_tag="OQN13_003847"
     CDS             complement(1015..1095)
                     /locus_tag="OQN13_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617062.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1131..1555)
                     /locus_tag="OQN13_003848"
                     /pseudo
     CDS             complement(1131..1555)
                     /locus_tag="OQN13_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1985..2475
                     /locus_tag="OQN13_003849"
                     /pseudo
     CDS             1985..2475
                     /locus_tag="OQN13_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2700..3191
                     /locus_tag="OQN13_003850"
     CDS             2700..3191
                     /locus_tag="OQN13_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCS1617063.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            3526..>3978
                     /gene="ygeH"
                     /locus_tag="OQN13_003851"
     CDS             3526..>3978
                     /gene="ygeH"
                     /locus_tag="OQN13_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HCS1617064.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKID"
BASE COUNT         1491 a          635 c          640 g         1212 t
ORIGIN      
        1 agatccataa tgacattaac atacaaaatc atgtaataaa ctcgtcaaca gacgcacata
       61 cacacaatgc cccacctgcc ataaaagtta atgcgggata caaagagagc attggtggcg
      121 caaagaattt caataacaaa atcctcaaac atataaaaag tcatctaatt atgttgagcg
      181 catttgtcat aggcgcatat tctgcatatt ggctatggaa taataatcaa ccgaagccat
      241 ttttcaagga ttacaagact gttgcggaaa ttaatggctg ccattttaat gtaacagaag
      301 atacaattga tgggttgaaa gagttcgata aatataaaac acggatactg gattcgggga
      361 ttaactgtaa aaaatatccg tggttatact tccctcttgc aaaatcctca ccggggatga
      421 ttgttatggc atgcaataaa aactataacc aacatgaagt ggcagactgt ttaaccctgt
      481 cttaccgtga ggttaatcgt gattgattat aaaaaaaact tattatttat tttagttttt
      541 atcagtgggt ttattctctt cactgtctat tcttacactg cagaaaaaat gatatataac
      601 gaaacatgca ctgcaaattg ggtaattttt aatgaccagg gacgagcaaa tcttaccatc
      661 gactttatgt ataacaaaaa aaataaaaca ggaactgttg cgttgagtgg aacctggcaa
      721 caaggtaaca gagaaagtaa atcaatacgg cgaaatattg aatacacatg gattgaaaac
      781 tatgacacag cccatttaac atcaaagaaa gttaataagt ttgagattat ggatcaagtt
      841 gatgatgata gacttgcaca acttattcct gatttttatg tctttccaga aaaaagtgta
      901 agctataata tactaaagca aggtaagcat gcttttattt tgagcattgg taacagagca
      961 ataatgcatt gtgcaaggta aactaaaata gataacaaaa aagatggaac ctcgttaatc
     1021 ataaaacaat aaaattaaat attctcgcag tatatggcag tctaaagcat caaagttttg
     1081 atcaacatct ttcattttag acatctcctt gcaatgtaaa atatcatgta tcaacaacat
     1141 ctggtgcaaa tccatgagtc gaactcgaca ttcatcttag ctcgattatt attccttcgt
     1201 acagtcgatg taaacaatac agaaagagga ttattaagaa ccagtttact caggaatcaa
     1261 tagaggaaag gactaacgtt tctttaaaag aattgatttc atcatctgtt aaactaaact
     1321 catcattgac agatcgtgag atataactgt ttttaacttt actctttacg ttgactttat
     1381 tgacagaatt aacattcaca tatcttgaat ttaatgtcca tgatgttgtt tgcgtgagaa
     1441 cattctcagc attaaggaat ttttttaacg gaatccttgg tttctttttc gagtccgaat
     1501 ttacaatatc atgcatatag accatttcat gaatctgcga aaactcaatg tccattgtat
     1561 acctcacatt tttaccgtga ctcgatgtta ctgttcaata atcaccttcc atcaatacta
     1621 aaattaatac ccctaatgtg ccgataacaa atatagtcat tctacgtaac gtctccataa
     1681 ggtgataacc ttgatcaaat gaatttcgaa tataataaag gaaatctttt gagatgttta
     1741 tcacgaataa agctatttat gttaccaata tcatttcgag tgcgcacact atctatctgt
     1801 tttttcgaga tataaaaaac aacgtgtaag ttatgtaaat tgccgatgct actgagtaac
     1861 attatggtaa aaaaagtaaa aatgagcgat ttttgataga ataaagcatg agaaaaggtg
     1921 atatttgaca ttatcagaag ctgcgaattc ggattttgct ctaatcaaat gagagaaatg
     1981 taaaatgaag ccacgaaata ttaataatag cctaccactg caaccattag ttcctgatca
     2041 ggagaacaaa aataagaaaa atgaagagaa atccgttaat ccagttaaaa tcacaatggg
     2101 gtctggttta aattatattg aacaagaatc tcttggagga aaatatctaa cacatgattt
     2161 gtcaataaag atagcggata tttctgaaga gataattcag caagcaatat tatctgctat
     2221 gagcatatat aaattttcga taacagatga tttaatgagt atggctgtaa atgaactcat
     2281 aaaactgacc aaaatagaga ataatgtaga cctgaataaa ttcactacta tatgcacaga
     2341 cgttctatcc cccctcgtca ccagacataa taaagaaaaa aaacaaacga cattctaccc
     2401 ttctcaaaaa tccccttttt aattttcatt gaaaaaatgg caattgatac tatgaatcca
     2461 ggtcatacac attaaggcat ttatgtaatg tcgtaattaa gataactaat aaggtgaata
     2521 ttagtaaaac aactaatgat gttattagtt tgtagtgaac agtactttta ccaataatga
     2581 aaaatatacc ataagcaatg caagcttaaa actaataatt taaaaaagat aaatgtaaaa
     2641 atattaatga gtcaatcaat aataatttag catcacgaga cacatcacag aggaatatta
     2701 tgagcacaga aacaattgaa atattcaata atagtgatga atgggcaaat caactaaaac
     2761 acgcattatc gaaaggagaa aatctggcat tactacatgg tttaactcct gatatccttg
     2821 atagaatata tgcatatgca ttcgactacc atgaaaaggg taatataacg gacgcagaaa
     2881 tttattataa atttttgtgc atttatgcgt tcgaaaatca tgagtatcta aaagattttg
     2941 catcagtatg tcagccaaaa aagaaatatc aacaagcata tgacctttac aaactaagtt
     3001 acaattactc cccgtatgat gactattcag ttatttatcg tatgggtcaa tgtcagattg
     3061 gggctaaaaa tatcgataac gcaatgcaat gtttctatca cattattaac aattgtgagg
     3121 atgatagtgt taagagtaaa gcgcaggcat atattgaact cttaaacgat aattcagaag
     3181 ataatggcta agcattacaa tcatttggtt aaatttgctg gttatagtgc agttaactac
     3241 tataaccagc acctcttatg ttttcttaaa tcagcatctt aaaacagtta agagcatact
     3301 ataaatcatt atttttcaat gcgttagaga acaatctact ttataaaaat tgaataaggc
     3361 tatttcagat tacatcactg aattcctaag ataaattagt gaacacaacc atagatatgt
     3421 cgaatgtatt ttcctgtagc attaatgaca cataaatatg aatagccata atttctattg
     3481 cttaagcagg atgcaagaaa ccaatttttt catagaggtt aactaatgga cttagaaaat
     3541 aaattctcat atcattttct tgagggatta acgctcacgg aagatggaat tcttactcaa
     3601 ggaaatgagc aagtttatat tccacagaaa gagttaggtg tattaatagt attacttgaa
     3661 tccgctggtc atgttgtact gaaagatatg atcatcgaat cagtatggaa aaatattatt
     3721 gttagtgacg agtccctgac aagatgtatc tattctttgc gctgcatttt tgaaaaaatt
     3781 ggctatgatc gttgcataga aacaatctac cggaaaggtt atcgtttcag cgggcaggtt
     3841 ttcaaaacta aaattaatga agataatact tcagactatt ccatagctat attccctttc
     3901 actacttcat tgaatacact ggatccatta atacttaatc aggaattagt gcaaatcatt
     3961 tcaaataaaa aaatcgat
//
LOCUS       DAJVRF010000224         3906 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJVRF010000224 DAJVRF010000000
VERSION     DAJVRF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.225"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..1358
                     /gene="dcuC"
                     /locus_tag="OQN13_003852"
     CDS             <1..1358
                     /gene="dcuC"
                     /locus_tag="OQN13_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS1617065.1"
                     /translation="VVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSSQ
                     ASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQY
                     INSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASPA
                     AIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEHI
                     SHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILEF
                     LRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSFG
                     SASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQASN
                     LGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVTG
                     K"
     gene            complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="OQN13_003853"
                     /gene_synonym="citB"
     CDS             complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="OQN13_003853"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCS1617066.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="OQN13_003854"
     CDS             complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="OQN13_003854"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HCS1617067.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHHNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT          926 a         1007 c          921 g         1052 t
ORIGIN      
        1 gggttgtggt tattgtgggt gtagctcgct acatcattaa agggtattcc gccactggtg
       61 tgttatttgt cggtggcctg ttattgctga ttatcagtgc cattatgggg cacaaagtgt
      121 taccgtccag ccaggcttca acaggctaca gcgccacgga tatcgttgaa tacgttaaaa
      181 tattactaat gagccgcggc ggcgacctcg gcatgatgat tatgatgctg tgtggatttg
      241 ccgcttacat gacccatatc ggcgcgaatg atatggtggt caagctggcg tcaaaaccat
      301 tgcagtatat taactcccct tacctgctga tgattgccgc ctattttgtc gcctgtctga
      361 tgtctctggc cgtctcttcc gcaaccggtc tgggtgtttt gctgatggca accctatttc
      421 cggtgatggt aaacgttggt atcagtcgtg gcgcagctgc tgccatttgt gcctccccgg
      481 cggcgattat tctcgcaccg acttcagggg atgtggtgct ggcggcgcaa gcttccgaag
      541 tgtcgctgat tgacttcgcc ttcaaaacga cgctgcctat ctcaattgct gcaattatcg
      601 gcatggcgat cgcccacttc ttctggcaac gttatctgga taaaaaagag cacatctctc
      661 atgaaatgtt agatgtcagt gaaatcacca ccactgctcc tgcgttttat gccattttgc
      721 cgttcacgcc gatcatcggt gtactgattt ttgacggtaa atggggtccg caattacaca
      781 tcatcactat tctggtgatt tgtatgctga ttgcctccat tctggagttc ctccgcagct
      841 ttaataccca gaaagttttc tctggtctgg aagtggctta tcgcgggatg gcagatgcgt
      901 ttgctaacgt ggtgatgctg ctggttgccg ctggggtatt cgctcagggg cttagcacca
      961 tcggctttat tcaaagtctg atttctatcg ctacctcgtt tggttcggcg agtatcatcc
     1021 tgatgctggt attggtgatt ctgacaatgc tggcggcagt cacgaccggt tcaggcaatg
     1081 cgccgtttta tgcgtttgtt gagatgatcc cgaaactggc gcactcttcc ggcattaacc
     1141 cggcgtattt gactatcccg atgctgcagg cgtcaaacct gggtcgtacc ctatcacccg
     1201 tttctggcgt agtcgttgcg gttgccggga tggcgaaaat ctcaccattt gaagtcgtaa
     1261 aacgcacctc ggtgccggtg cttgttggtt tggtgattgt tatcgttgct acagagctga
     1321 tggtgccagg aacggcagca gcggtcacag gcaagtaaat agtaatgccg gaggcaagtt
     1381 tcctccggct ttatcatgtc acccactgtg gtatatgcgt tgtggtctgc caactttgcc
     1441 gtgaacaatt tcagcaataa tcagatggcg gctggcgcaa tattcaagat aacgcctggc
     1501 agtggtgcag ctgatggtca gtgcctgcgc caccgtttct gccgtatgtt gcacaccagg
     1561 ctctttaaac agttttcgca ccgcgtttag cgtcaagggg tcaatgccgg tcggtagctc
     1621 gtccttaggt tcaccgcgag cataagcatt aaacatctca tcaatttgct tctggctggc
     1681 gctatcaata ctttccagca tatgtttacg ctggcggaaa cgggttagcg tttgccccag
     1741 ccgttcatag gcaatgggct taatgagata atcaaataca ccacaacgta cggcttcaga
     1801 caccgtttcc atatcgctgg ctgcagtggt aaacaccacg tcgccgggat aatgcgcctg
     1861 caccagttca tgcagtaaat taatccctct accgtcagga agatagttat cgagcaagat
     1921 tagccccggc ttaaaacgct cgatcatcat tcgggcctgc gccagatttc ccgccagtaa
     1981 tatctgactg aatccgggaa tgtgacgaat atattccgca tgcatctctg ccagcggcgt
     2041 ttcgtcctca acgatcaata gggttaatgg agctgtcatt aggtttcact ttcggaatat
     2101 agattgaaaa taaggtaccg cagggatcat tatcttcgag agtgataaca ccaccgcagc
     2161 gcgttacgta gctggcaatc aagtacaacc caatgccatg ttcaccgggc tcgtcagcac
     2221 gcgtactgac cccctgctca aatattttgt ctcttagaga ctctggaacg ccgcagccct
     2281 gatcggcgac ttcaatcacc acatcatcgc cttcatcgct gaggaataat tcaacgatct
     2341 tgtttccttc atcgctacgc aggctggctt cgaaggcgtt atcaagtaaa ttgccaacaa
     2401 tggcggcaaa ctcggtgcta tccagtcctg gcggcagttg cgaaagctgg ctaccgggga
     2461 cgatgatcat ttttagcccc agttcgcggg cgcgctgcac tttaccaaaa agcagccccg
     2521 ccacctggcg atcggcaaac gcctcacgca ggctgtcaat aagctgttgc tgggcctgag
     2581 actccccctg caccatcgcc agcacgcgat catactcttt catctgcaac agaccattga
     2641 gcgtcgacat ccaattgtga tgctcgtgtc gcaatgtacg aaggctctcg acgtattgtt
     2701 ttatttgcgt cagttgcgca ttgagggtgg aaatttcgtc tttactacga aagctgataa
     2761 tggcccccag caaatcatca cctgaacgaa tagcttcccg gttggcaata acgctcagac
     2821 cattaaagtt cgccaccaca tcctgacgtt tttcatcaat ctgttcggta aagaaatcgg
     2881 cgggcctgac cacttcagca atgggtttac ccaaccattg ccgtccggga gagctcagcc
     2941 ccagcatctt tcttgcgtta cgattgatgg cggtaatgta accatgcgga tccaccgcaa
     3001 tcagcccttc ataaaccgaa ctaaacagcg cctcttgctg acggaccacg cgtgcgattt
     3061 gctttggctc catgcccatc atttgtcgac ggatatgcgc ggccaggaac cacgacagca
     3121 acatcagaat ccctaacagc acgacaaaca cgcctgccat cggtaataaa aactcagccc
     3181 gccagctatc gattttactc accaggtagc caatcgacac cacgccgatg acttttccat
     3241 cgtcatcaaa gattggcgtt ttggcgcgca tcgccatccc cattgcccct ttcccggtaa
     3301 tgaagtagct ctcccctttc tccagcgcgc cctgtttggt gaactgcata ggataaccaa
     3361 ttttctccgg attaagatgg taaaggcgga tcgagtgccg gtcgccaatc accacataat
     3421 caaaatcggt atctctttgt aatttgttag cgatggtcgc cagccgtttg tagtcacgcg
     3481 ttttcaccgc agagatgaca ctgtcattgg aggcaataat tttcgcctga ttcattgcca
     3541 tgtcgcgtac atgaagcgtt aaatagtcct caaaactggc cgtaaaatat tgcgccaggg
     3601 ctgcaatgac aaatattgag aacaccagaa tcagcaaaaa gatacgcagc ggaaatgcca
     3661 gtctttggaa aaatgcaaac tgtttattct cgttaagctg caacattgtt ttccttgcgg
     3721 gttgtcccga gcctctttga cggatgtaat taaaatgttg aatcaaaata catagatatt
     3781 aattatgatc gtggcaaatt ttattcatta aatcaattaa atcacttaaa cctgcaggtc
     3841 atttcgtaat tacattaatt aaaaaactta aaaccattaa ataaataaaa aaccactaac
     3901 tctatg
//
LOCUS       DAJVRF010000225         3892 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJVRF010000225 DAJVRF010000000
VERSION     DAJVRF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.226"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..746
                     /locus_tag="OQN13_003855"
     CDS             <1..746
                     /locus_tag="OQN13_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCS1617068.1"
                     /translation="GLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVLTYYV
                     IGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSLLEKH
                     IYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYAL
                     AAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLA
                     LNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            736..1806
                     /locus_tag="OQN13_003856"
     CDS             736..1806
                     /locus_tag="OQN13_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS1617069.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVRHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            1806..3554
                     /locus_tag="OQN13_003857"
     CDS             1806..3554
                     /locus_tag="OQN13_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HCS1617070.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(3603..>3892)
                     /locus_tag="OQN13_003858"
     CDS             complement(3603..>3892)
                     /locus_tag="OQN13_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HCS1617071.1"
                     /translation="FDVDAAMKKVSELETLVAQAKEADKGGMNFSFINSADQYQLETK
                     KYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT          904 a          985 c         1016 g          987 t
ORIGIN      
        1 tggggctggc gattggctat ttcgtcttct ggttccgcaa agtgcgcctt ggcaaagcat
       61 tacaaccgct gctcggttca atgctgatcc cgttcgttac tctgctggtt ttcggcgtgc
      121 tcacttacta cgttatcggg ccggtgatgt ccgacctcat gggcgggctg ctccacttcc
      181 tgaataccat tcctccatca atgaagtttg cggcagcgtt tctggtcggt gcgatgctgg
      241 cgttcgatat gggcggcccc atcaataaaa ccgcctggtt cttctgcttc tcactgctgg
      301 aaaaacacat ttatgactgg tacgccatcg tcggcgttgt cgcgttgatg ccgcccgttg
      361 cggcgggtct tgcgacgttc attgcgccca aactgtttac ccgacaggaa aaagaggctg
      421 ccagtagtgc cattgtggtc ggtgccacag tcgcaaccga accggctata ccttacgctc
      481 tggcagctcc gttgccgatg attaccgcca atacgctggc gggtggcatt accggcgtgc
      541 tggtgatcgc tttcggaatc aaacgtctgg cacctggttt gggtatcttt gacccgctga
      601 tcggcctgat gtcgccggtg ggttcgtttt atctggtgct agcgattggc ctcgcgctga
      661 acatttcatt cattatcgtc ctgaaaggat tgtggttacg ccgcaaagcg aaagccgcgc
      721 aacaggagct tgtccatgaa cattgagtta ctgcaacagt tgtgcgaagc cagcgccgtc
      781 agcggcgatg aacaggaagt tcgcgacatt ctgataaaca cgctggaacc ttgcgttaat
      841 gagatcacct ttgatggtct gggcagcttt gttgcccgta aggggaataa aggtccaaaa
      901 gttgccgttg tcaggcatat ggatgaagtc ggctttatgg tcacccacat cgacgagagc
      961 ggttttctgc gctttaccac cattggcggc tggtggaatc agtcaatgct caaccaccgg
     1021 gtaaccatac gcacacacaa gggatttaaa atccctggtg tgattggttc cgtcgcacct
     1081 catgcgttaa cggaaaagca aaagcaacag ccgctgtcat ttgatgagat gttcattgat
     1141 attggcgcga acagtcgcga tgaggtggaa aaacgcggtg tggcgatggg cgattttatt
     1201 agcccggaag ccaattttgc ctgctggggc gaagataaag tggtcggcaa ggcgctggat
     1261 aaccgcatcg gctgcgcaat gatggctgaa ctattgcaga cggtgaataa tcccgaaatt
     1321 acgctgtatg gcgtcggcag cgtggaagaa gaagttgggc tacgcggggc acaaacctcg
     1381 gcggaacaca ttaaaccgga tgtggtgatt gtgctggata ccgctgtcgc aggtgatgtt
     1441 ccgggcattg ataacattaa atacccgctg aaactgggcc aggggccggg gctgatgctg
     1501 tttgacaagc gctacttccc caaccagaaa ctggtggcgg cgttaaaaag ctgtgccgca
     1561 cataacgatt taccgctgca attttccacc atgaaaaccg gtgcgacgga tggcgggcgc
     1621 tacaacgtga tgggcggcgg gcgtccggtt gttgcgctgt gcctgccaac ccgctatctg
     1681 cacgctaaca gcggcatgat ttcaaaagcc gattacgatg ctctgctcac gttgatacgg
     1741 gattttctga ccaccttaac cgcggagaaa gtcaacgcgt ttagccagtt ccgtcaggtg
     1801 gattaatgct aaacgaacgc cagttaaaga ttgtcgatct gctggagcaa cagccgcgca
     1861 cgcctggtga gctggcgcaa cagactggcg tttcaggcag gaccatcctg cgtgatattg
     1921 actatctcaa ctttaccctt aacggcaaag cccgcatttt cgccagtggc agtgcgggct
     1981 atcagctgga aatcttcgag cgccgcagct tttttcagtt gctgcaaaag cacgataacg
     2041 acgatcggct gctggcgctg ttattactga atacctttac tccccgtgcg caactcgcct
     2101 cggcgcttaa tttgccagaa acctgggtag cagagcgtct gccccggtta aaacagcgtt
     2161 atgaacgcac ttgttgcctg gccagtcgcc ccggtttggg ccatttcatt gatgagacag
     2221 aagagaaacg cgttatcttg ctggcgaact tgctgcgcaa agatccgttt ttaattccgc
     2281 tggcgggcgt aacacgagac aattttcagc atttatccac ggcctgcgac aaccaacacc
     2341 gctggccgct catgcagggt gattatctct ccagcctgat tctggcgatt tacgccctgc
     2401 gtaatcaact gaccgatgag tggccgcaat atcccggtaa cgagataaaa caaatcgttg
     2461 aacagagcgg catgtttctt ggtgataacg ctgtaagaac cctgacaggt ttgatagaga
     2521 aacagcatca gcaagcgcag gtaatttcag ccgatcatgt gctggggttg ctgcaaaggg
     2581 tgccaggcat cgcgtcattg aatattattg atacgcagct ggttgagaat attaccgggc
     2641 atttattacg ttgccttgcc gcaccggtgt ggattgccga gcaccgccaa agcagcatga
     2701 ataacctgaa agccgcctgg cctgcggcct ttgatatgag tctgcacttt attacgctac
     2761 tgcgtgaaca gctcgatatt ccccttttcg atagcgatct gatcggtttg tattttgcct
     2821 gtgcgctgga acggcatcaa aacgaacgcc agccgatcat tttgctctcg gaccagaacg
     2881 cgattgccac tattaatcag ctcgccattg agcgcgatgt tttacattgt cgggtgatta
     2941 ttgcccgtag cttaagcgag cttattgcca ttcgcgaaga gattgagccg ttattgatca
     3001 ttaacaacag ccattattta ctggatgacg cggtaaataa ttacatcacc gtaaaaaata
     3061 tcattacggc tgccgatatc gaacaaataa aacactttct ggcgacggca tttattcgcc
     3121 aacagccgga acgctttttc tctgcccccg gaagttttca ttattcgaat gtacgcggtg
     3181 aaagctggca acatattacc cggcaaattt gtgcgcaatt agtggcacaa caccatatta
     3241 ccgccgatga agcgcaacgg atcatcgccc gcgaaggcga aggtgaaaac ctgattgtta
     3301 atcgcctcgc catcccacat tgctggagcg aacaggagcg acgttttcgt ggatttttta
     3361 ttaccctcgc ccaaccagtt gaggtgaata acgaagtcat taaccatgtc ttgatcgcct
     3421 gcgccgccgc cgatgcgcgt catgagctaa aaatatttag ctatctggca agcgtattgt
     3481 gtcagcatcc ggcagaagtt attgccgggt taacaggata tgaggcattt atggagttac
     3541 ttcacaaggg gtgaactgtg tcggatgcgc cgcatccgac actgcatttt catcaaaaac
     3601 gtttaacgca ggctattata gtcatcaacc atttcgttgt actcgcgtaa cgctcgtgga
     3661 aatgagtctt cgaccatcca gcttgtgttt gcatcctgaa gatgctcttt atcccagtca
     3721 gagtacggga ctttatctct gacgcggcga acatattttt tagtctcgag ttgatactgg
     3781 tctgccgaat taatgaacga gaaattcata ccgcctttat ccgcttcttt ggcctgcgct
     3841 accagcgttt ccagttcaga gactttcttc attgccgcat caacatcaaa ct
//
LOCUS       DAJVRF010000226         3799 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJVRF010000226 DAJVRF010000000
VERSION     DAJVRF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.228"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(99..908)
                     /gene="proC"
                     /locus_tag="OQN13_003859"
     CDS             complement(99..908)
                     /gene="proC"
                     /locus_tag="OQN13_003859"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCS1617072.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1028..1486
                     /locus_tag="OQN13_003860"
     CDS             1028..1486
                     /locus_tag="OQN13_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCS1617073.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1669..2193
                     /gene="aroL"
                     /locus_tag="OQN13_003861"
     CDS             1669..2193
                     /gene="aroL"
                     /locus_tag="OQN13_003861"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCS1617074.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2243..2434
                     /gene="yaiA"
                     /locus_tag="OQN13_003862"
     CDS             2243..2434
                     /gene="yaiA"
                     /locus_tag="OQN13_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCS1617075.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2692..3369
                     /locus_tag="OQN13_003863"
     CDS             2692..3369
                     /locus_tag="OQN13_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCS1617076.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3441..3725
                     /gene="ppnP"
                     /locus_tag="OQN13_003864"
     CDS             3441..3725
                     /gene="ppnP"
                     /locus_tag="OQN13_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCS1617077.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          951 a          904 c          990 g          954 t
ORIGIN      
        1 tcgtgagtgg ttgaaatcgg cagatttggc gctttacaaa gcaaagaaag ccggacgtaa
       61 ccgcaccgaa gtggcggcct gacgtccggc gaaagtcatc aggatttgct gagtttttct
      121 gatttttcca tacacttcgt catcgcttcg atcactgcgg cacggaagcc tttctcttcc
      181 agtacgcgta ccgcttcaat ggtggtgcct cccggtgagc agaccatatc tttcagtgcc
      241 cccggatgtt ctcccgtttc cagcaccatt tttgcggaac ccattaccgc ctgagcggca
      301 aatttatacg cctgggcgcg tggcatcccg cccagcacgg cggcgtcggc catcgcttcg
      361 ataaacataa atacgtaggc tggcgaagaa ccgctcacac cgaccaccgg gtggatcatc
      421 ggctcagcaa ttacttccgc ttcgccaaag cagcggaaaa tattcagcac atcagcggta
      481 tcttcggggg ttaccagcgc gtttggcgtt acggaggtca tcccggcatt aaccagcgcg
      541 ggagtgttcg gcatggcgcg gataattttc cggtcatggc ccagcgcgcg ggcaagctgg
      601 tcgagcgtga cacctgcagc aatagaaacg accagagagt ctttattcag gctggaggtg
      661 atttcgctaa gcactttaat catgatgcca ggtttaacgg cggcaaaaat gatgtcggcg
      721 atttgcgcca cttcttgcgc cgattctgcg gcgttgatgc cgaactggtc atgcagggcg
      781 gcgactttat ccggggaggg ggtgtatacc cagatttgcc ctggaagcac ctgaccgctg
      841 gcaatcagac cgccgagaat ggcttttccc atattgccgc agccaataaa accgattttc
      901 ttttccattg cctcactcct gccgtgaaat tcattgtttt gataatcgct ggcagaagca
      961 taaacagaac tatgccggaa ggcaaaagcg cgacacaata gaggattacc caacaaagga
     1021 tgactttatg acaatttggg tggatgccga cgcgtgtccc aatgtaatta aagagatttt
     1081 gtatcgcgcg gcggaacgta tgcagatgcc gctggtactg gtagcaaacc agagtttacg
     1141 cgtgccgcca tcgcgattta ttcgtacgct gcgcgtcgcg gcaggtttcg acgttgccga
     1201 taacgaaatt gtccggcagt gtgaagcggg cgatttggtg atcaccgcag atataccttt
     1261 ggctgctgaa gccatcgaga aaggcgcagc ggcgcttaat ccgcgcggcg aacgttacac
     1321 gccagcgacc attcgtgagc gcctgacgat gcgcgatttt atggatacct tacgtgccag
     1381 tgggatccag accggcggac cagatagcct ttcacaacgt gaccgccagg cctttgccgc
     1441 ggagctggag aagtggtggc tggaagtgca acgtagtcgt ggctaaatgt aatttattat
     1501 ttacacttca ttcttgaata tttattggta tagtaaggga tgtattgaga ttttcacttt
     1561 aagtggaatt ttttctttac aatcgaaatt gtactagttt gatggtatga tcgctattct
     1621 catgacaccg gctttcgccg cattgcgacc tattggggaa aacccacgat gacacaacct
     1681 ctttttctga tcgggcctcg gggctgtggt aaaacaacgg tcggaatggc ccttgccgat
     1741 tcgcttaacc gtcggtttgt cgataccgat cagtggttgc aatcacagct caatatgacg
     1801 gtcgcggaga tcgtcgaaag ggaagagtgg gcgggatttc gcgccagaga aacggcggcg
     1861 ctggaagcgg taactgcgcc atccaccgtt atcgctacag gcggcggcat tattctgacg
     1921 gaatttaatc gtcacttcat gcaaaataac gggatcgtgg tttatttgtg tgcgccagta
     1981 tcagtcctgg ttaaccgact gcaagctgca ccggaagaag atttacggcc aaccttaacg
     2041 ggaaaaccgc tgagcgaaga agttcaggaa gtgctggaag aacgcgatgc gctatatcgc
     2101 gaagttgcgc atattatcat cgacgcaaca aacgaaccca gccaggtgat ttctgaaatt
     2161 cgtagcgccc tggcacagac gatcaattgt tgattttaga gcgcctatac ttaacgttca
     2221 tcccgtgaaa taaggaagaa cgatgccaac gaaaccgcct tatcctcgtg aagcatatat
     2281 agtgacgatt gaaaaaggaa agccaggaca gacggtaacc tggtaccaac tcagagccga
     2341 tcatcctaaa ccagactcgt tgatcagtga acatccgacg gctcaggaag cgatggatgc
     2401 gaaaaaacgc tatgaggacc ctgacaaaga gtgaccgcat cagactgctc ggaagggatt
     2461 ctgagtgcca ctacaaggga tctgcgtcac atttttcata attcatgttt ttctaataat
     2521 tagaatatta aacaataaca atccattact ggaatcattt ggaatcttta cattatgccg
     2581 tgcacgtctg ctgctacgct ttttgtcatt tgtagcacaa gtaagtgtca gcagtggtgc
     2641 tccacacttg cccggtaatt aacgacgaaa gaaaagtaag gtggatgaac aatgagtgcg
     2701 tcgttggcga tcctgaccat cggcattgta cctatgcagg aggttttgcc gctcctgacg
     2761 gaatacattg acgaagataa tatttcccat catagcctgc tggggaagtt aagtcgtgaa
     2821 gaagtgatgg cggagtacgc gccagaagca ggcgaagaca ccattctcac attattaaat
     2881 gacaaccagc tggcccatgt ttcgcgtcgt aaagtggagc gagacctgca aggtgtggtt
     2941 gaagtgctcg ataatcaggg ttatgacgtc attttattaa tgagtacagc aaacattagt
     3001 agtatgactg cgcgtaatac gatctttctt gagccgtcgc gaatattgcc tccactggtt
     3061 tcctctattg ttgaagatca tcaggtgggg gttatcgttc cggttgagga gatgctgacc
     3121 gttcaggcgc aaaaatggca aattttgcag aaatcgccgg tattttcatt gggtaacccc
     3181 attcatgatt cagaacaaaa aatcattgat gccgggaaag aattactggc aaaaggggct
     3241 gatgtcatca tgctggattg tttaggattt caccaacgtc atcgcgattt actgcaaaaa
     3301 cagctcgatg ttcctgtctt gctgtctaac gtattgattg cacggctggc tgcggaatta
     3361 ctggtgtaat tttgcgtgac agccagcgcc tctggcccct atagtgaagt agatgttcaa
     3421 ctaccagaca gggccagttt atgcttcaaa gtaatgagta cttttccggc aaagtgaaat
     3481 caatcggctt ttccagcagc agcactggtc gcgccagcgt gggtgttatg gttgaaggcg
     3541 aatacacctt cagcaccgct gagccggaag agatgacggt aatcagtggc gcgctgaatg
     3601 tgttactgcc tgacgcgacc gactggcagg tgtatgaagc cggttcggtg tttaatgttc
     3661 ccggtcacag tgagtttcat ctgcaagttg ccgaacccac ctcttatctg tgccgctatc
     3721 tgtaattcct cgccttcccc ttgaacggga gggcattttt ctgaaatatc ctctctttaa
     3781 cccataataa tatttcctt
//
LOCUS       DAJVRF010000227         3795 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJVRF010000227 DAJVRF010000000
VERSION     DAJVRF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.229"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..738
                     /gene="adeQ"
                     /locus_tag="OQN13_003865"
                     /gene_synonym="yicO"
     CDS             <1..738
                     /gene="adeQ"
                     /locus_tag="OQN13_003865"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCS1617078.1"
                     /translation="SSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGE
                     VDLSGALTLELAGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSV
                     SSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYAT
                     AGALIFVGVLMTSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKV
                     CTGRWRDLNLCVVVVASLFALKIILVD"
     gene            791..1243
                     /locus_tag="OQN13_003866"
     CDS             791..1243
                     /locus_tag="OQN13_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCS1617079.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1454..2634
                     /gene="nepI"
                     /locus_tag="OQN13_003867"
                     /pseudo
     CDS             1454..2634
                     /gene="nepI"
                     /locus_tag="OQN13_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2675..2968)
                     /locus_tag="OQN13_003868"
     CDS             complement(2675..2968)
                     /locus_tag="OQN13_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617080.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3190..>3795
                     /locus_tag="OQN13_003869"
     CDS             3190..>3795
                     /locus_tag="OQN13_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family lipoprotein"
                     /protein_id="HCS1617081.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVALGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAII"
BASE COUNT          816 a          861 c         1007 g         1111 t
ORIGIN      
        1 tcatcacgtc attttcatgc cgcggtgctg gtttctattg tggtgacgtc ttgctgtgga
       61 ttatttttcg gtgatgttca ttttagcggc gtctattcca ttccgcctga tattagcggc
      121 gtcattggtg aagtagattt gagcggcgcg ttaacacttg aactcgccgg tatcattttc
      181 tcctttatgc tgatcaacct atttgattca tcaggaacat taattggtgt aactgataaa
      241 gcgggcttaa tagatggtaa cggtaaattc cccaatatga ataaggcgct gtatgttgat
      301 agcgtcagtt cggtggcggg tgcgtttatc ggcacctcgt ctgttactgc ctatattgaa
      361 agtacttctg gtgtggcagt cggtggccgc acggggctga ctgcggttgt ggttggcgtt
      421 atgttcctgt tggttatgtt cttctccccg ctggtggcga tggttcctcc ttacgcaacc
      481 gccggagcgt taatctttgt tggcgtgctg atgacttcga gcctggcgcg cgttaactgg
      541 gatgatttta ccgaatcggt gcctgcgttt attaccacgg tgatgatgcc ctttactttc
      601 tcgatcaccg aagggattgc actcggcttt atgtcgtact gcatcatgaa agtgtgcacc
      661 gggcgctggc gcgatctgaa cctgtgtgtg gtggtggtcg catctctgtt tgcactgaag
      721 attattctgg tggattagta actggcgagg gtttccagat atcatgagtt ctgataacgc
      781 aggagaactc atgatctgga taatgctcgc cacgctggcg gtagtgtttg tggttggttt
      841 tcgggtgctg acatccgggg ccagaaaagc gattcgccgt ctcagtgatc ggctgaatat
      901 cgatgtcgta cccgtggagt cgatggtcga tcaaatggga aagtcagccg gtgacgaatt
      961 tttacgttat ttgcatcgcc cggatgagtc gcacctgcaa aacgccgcgc aggtgttgct
     1021 catctggcaa attgtcattg tcgatggtag cgaacagaac ctgctgcaat ggcatcggat
     1081 tttacaaaaa gcccgccttt ccgcgccgat taccgatgct caggtcaggc tggcgctggg
     1141 ttttctgcgc gaaaccgaac ctgaaatgca ggatattaat gcttttcaga tgcgctataa
     1201 cgcgttcttt cagcctgccg agggcgttca ctggctgcat taaaaccgct tcgtcttatc
     1261 agcctacaga ccgcgcctga cctgtcgggt ggataagacg ttcacgccgc atccggtaat
     1321 ctttgccctg ttccgtttcg atcttaactc cagctcaagc aacaccgtaa aagttgcaaa
     1381 aacgttaaat gcgtcacaca tttcaatgtt aaagtggtcg gcttttcccc tgaaacatgc
     1441 cacgggtaac accatgagtg aatttattgc cgaaaaccgc ggcgcggatg ccatcacccg
     1501 accgaactgg tcagccgttt tctcggtggc gttttgtgtc gcctgtctga ttatcgttga
     1561 gtttttgccc gtcagtttgt tgacgccaat ggcccaggat ttaggcattt cggaaggggt
     1621 tgccgggcaa tcggtgaccg tgaccgcctt tgtggcaatg tttgccagtt tgtttattac
     1681 ccagacaatt caggctactg accgccgcaa cgttgttatt ttgtttgccg ttttgctgac
     1741 gctctcctgc ttgctggttt cctttgctaa ctcattcagt ttgcttttaa tcggtcgtgc
     1801 ctgtctgggg ctggcgctgg gcgggttctg ggcgatgtcg gcgtcgctga ccatgcgtct
     1861 ggtgccgccg cgtacggtgc cgaaggcgct gtcggtgatc ttcggcgcgg tttctattgc
     1921 gctggtgatt gccgcgccgt tgggcagttt tttaggcgag cttatcggtt ggcgcaatgt
     1981 ctttaatgcg gcggcggtga tgggcgtgct gtgtattttc tggattatca aatcattgcc
     2041 ttcactgcca ggcgaaccct cgcatcagaa acaaaatact ttccgcttat tacaacgtcc
     2101 gggtgtgatg gcagggatga tcgccatctt catgtctttc gccgggcagt ttgctttctt
     2161 cacatatatt cgcccggtgt atatgaacct ggcgggattc agcgtggatg gcttaacgct
     2221 ggtgctgttg agttttggca ttgccagctt tatcggtacg tcgctttcgt cattcattct
     2281 taaacgttcg gtaaaactgg ccttagcagg cgcgccgtta atactggctg tgagtgcgtt
     2341 ggtgctgacg ttgtggggaa gcgataaaat cgttgctacc ggcgtggcga ttatctgggg
     2401 gctaactttt gcattggttc ccgtcggctg gtcaacgtgg atcacccgct cactggccga
     2461 tcaggcagaa aaagccgggt ctattcaggt ggcggttatt cagcttgtgg cgcggcaatc
     2521 ggcggttatg cgctggataa tattggtctg acttcgccgt tgatgttttc tggcacattg
     2581 atgttgctga ctgcattgtt ggttactgca aaggtgaaaa tgaagaaatc ctgatatcag
     2641 gtaaagaagc gttggtcagt agttgttgct gttcttacat atccgggcat tctaccttac
     2701 ccaacgcttc ccaataattg ctctgattat tacgttccat cgccacgatg gcctcgcgta
     2761 tatgttgttt attgttttca tctgacaaca ttaacttttc ccacgcttca tctgataatg
     2821 acgcctgtgg cgtgcaaatt ttacgtaaat cttgtaatac cgtggttttc tcttttgcgg
     2881 actgcagtga gctaaaaggc gactcggcgt atacgatgcc tggcatagcg aaaaaagtga
     2941 aaataagcag tagtgtcgtt ggtttcatgg attcaacctt ttacaaatgt gtatcgaatg
     3001 aagcataagt gtagtttgct ttcatcgctc tcatcatgct gtacgaaacg cgcttgcgtg
     3061 gtcagtaaga agtgccagac tttatattcc acttttattc ctttttattc ttatcgatag
     3121 cgtttcgttt tttaaaccgc agcgacctta ccgctatagt caggtaatca ttaataaaag
     3181 gataaaaaaa tgaaactgac aacacatcat ctacggacag gggccgcatt attgctggcc
     3241 ggaattctgc tggcaggttg cgaccagagt agcagcgatg caaaacacat taaagttggc
     3301 gttataaatg gcgcagaaca agatgtcgcg gaagtcgcta aaaaggtggc aaaagagaaa
     3361 tatggcctcg atgttgaact ggtaggattt agcggttcgt tgctaccgaa cgatgcgaca
     3421 aatcatggcg aacttgatgc gaatgtcttc cagcatcgac cctttcttga acaagataat
     3481 caggcgcatg gttataaact ggtagcgctg ggaaatacgt ttgtcttccc tatggcgggt
     3541 tattccaaaa aaatcaaaac cgtggcgcaa ataaaagaag gcgcgacagt ggcgatcccc
     3601 aacgacccga ccaaccttgg tcgcgcactt ttactgttgc aaaaagagaa gctaattact
     3661 ctgaaagagg gaaaagggtt attacctacc gcgctggata taaccgataa tcctcgtcat
     3721 ttgcagatta tggaactcga gggggcgcag ctcccgcgcg tactggatga tcccaaagtt
     3781 gatgtagcga ttatc
//
LOCUS       DAJVRF010000228         3785 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJVRF010000228 DAJVRF010000000
VERSION     DAJVRF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.231"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..369)
                     /locus_tag="OQN13_003870"
     CDS             complement(<1..369)
                     /locus_tag="OQN13_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCS1617082.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIH"
     gene            complement(375..908)
                     /locus_tag="OQN13_003871"
     CDS             complement(375..908)
                     /locus_tag="OQN13_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCS1617083.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(886..1974)
                     /locus_tag="OQN13_003872"
     CDS             complement(886..1974)
                     /locus_tag="OQN13_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCS1617084.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLVLVLGENILSCDTERSIRERFSEYQVSGQRPG
                     NDDDFGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRE
                     TTYTVQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGP
                     ADAYLPEPVTARKKKMLRRISDERVFSS"
     gene            complement(1971..3362)
                     /locus_tag="OQN13_003873"
     CDS             complement(1971..3362)
                     /locus_tag="OQN13_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HCS1617085.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRKSLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
     gene            complement(3409..3615)
                     /locus_tag="OQN13_003874"
     CDS             complement(3409..3615)
                     /locus_tag="OQN13_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617086.1"
                     /translation="MTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAG
                     VNVNGESGVTYENCKLNMKDINDD"
BASE COUNT          912 a         1097 c          893 g          883 t
ORIGIN      
        1 gtgaatattg ccgtcacgct ggtaaatggt aatggcaagt gccagcgagt ctgtcccggt
       61 ccggacagcg ttaatatcaa gacgggaagc aacaccatcc tccaccatcc acgccagcgc
      121 ctcacggaca taatctctgg caagctgcgg ggttttattt gtcagcttgc tgcgtcgcaa
      181 cagatacaaa cgcgaccccg tgcgatcatt ctgaaccgca ggccaggtat ccccccacca
      241 tccaaatatc cgtggtgcat catcacgccc ggcacgccgc catgtaaaaa gcgaaataat
      301 cacagcacgc gtcagaaggt cgatcgaagc cccggtcgac acgggtcgcc cgttaacatt
      361 aatcatcatg attttcagcc catcggttga tccggcgtgt cagtgatccc gccaccatca
      421 ccattttcgg tatgtttgtg ggcattgtag gcctgccgca tttgctgcat gctgagtccg
      481 ccactgtcgc aattgtcagt aatatcggcg gtggattcga ctggcatttc aaagcgcgct
      541 ttaggcgcgt ttttaaaaat tatggccttc ccggccccgt ccaccacaat accggaacgg
      601 gtcaggacaa cggactgtcc cagatcgtca taaagcgcca cttccccacg tttcagtcct
      661 ttcagccggt aacgtctgtc agccacgacc acaaccacac catgagaacg atcgcccgcc
      721 ggaaataaag caacgccctc agcaccgttc tgtgcggcag atgtaaaacc ataaggttca
      781 aggtgctcaa cgtgttgttt ctgatctccg gctatcattt tcaaccctac agactgacac
      841 tttctggcgg aatccaccgc cgtgatgaca gcccgggaaa tcagattacg aagagaaaac
      901 ccgttcatca gaaatcctcc tcaacatttt tttcttcctg gcggtaacag gctcggggag
      961 ataagcatct gccgggccaa cccgtaattc agtcgtcgtg ccccggtcgt cctggttata
     1021 ggtaacttct gcgatcacca gttcatcatt atcaaaatta ttcagcgggt cgaaaacgat
     1081 gacagataac cccggacgcc ataacgcgcc actgccctgc cgccagccct gaaccgtata
     1141 tgttgtctca cgggtaagcg cagcccgttg gcgcgcttca aattcacaac gggccttaca
     1201 ggttgctgtc gttgctgtgc ctgactgctg aatcaacaaa gggcgataac gggtcacgcc
     1261 actgtcctga atggtctgac gtattgcggc aatggtggcc tcaccaaaat catcgtcgtt
     1321 gcccgggcgc tgcccactga cctgatattc agaaaaccgc tctctgatgc ttctttccgt
     1381 gtcacaggaa agaatattct ctcccagcac cagcaccagc accagcgccg tcgccgcttt
     1441 acctgttccg ggttttccca gaaccagtcg cccgcattca tcgtcataag ccagcgtctg
     1501 aacctgcccc agtaaccggt taagacaatc ggcaacggtt tcgccgtgtt ccggctgggc
     1561 atcaatcacc gctgtctgcg gcacgccagc atcaacaacg gtgatgccaa atggcgcagc
     1621 cagttcactg actattctga gcaggttttt tccgctctgc tggagtggca aagcagagca
     1681 gtcaaccaga tcggctgttt tgcttcgccc gacaattccc atgctgacgc tgctggcgtc
     1741 ataacgaagc ggtagtgcct ccacatatcc ggtgagcaca ggctcatccc cgataagcac
     1801 ttcaaccagc tcaccatttt ttatccgggg ttgataatcc cggcttccgg gccagcgggt
     1861 ggtaatggca acattaaaat cccgggcaat acggttaatg cccgcactga tacggacgga
     1921 tgtccagccg ccccattcgc gaccggaaac ccgaagtaaa acggtatcat tcatctgacg
     1981 ggtaccctta atgccctgac cggaacaaag cccggatgag agatggcatt tcgatccagg
     2041 atatcagttt cacgggacgc atcgtcgtac catgacgcag ccagaacaag cgcaggcaga
     2101 acttccgctg gcgttcgctc tgcggtttcc tccgtctgaa ccagacgcgc ctgaatatcc
     2161 cggttcagtt ctgtacgtaa tgacgtcagc tgaaaaaaca gcctgtcatc cgccgtacgc
     2221 ctgagctcct gttcaatcgc cgcgttcagc gatttgcgta tgatggtgag attttcccgc
     2281 gtgggtggtt gcgctctctc atcctgtcct gtgctggctg ccacgctgtc aagtgccgga
     2341 tgagaaacgt gaataatatc ggactgacgt tcagtggaac caccaacagc cacaaccgcc
     2401 tgctgatttt tcaccaggct tccgggttgt ggcagtgaag tgaccgccct tgtggcttcg
     2461 ctgattgccg tcgtccggat gacagcagcc acaaggtttg tctgctgttt ttgcctgata
     2521 accgatgcgg aatctgtggg ccacaccgca cgcggtgcca gtcctgggtc cagcgtaatg
     2581 ccggacattg tcgttatgga ctgcaccaga tcctgtgtgt tatccaccag ccgggttccg
     2641 gcccgccagg tatcctgcaa cgtatgcaca aaatcactgg caaccgacgg cggcatcaga
     2701 atgacggata aatcgccctg cagtagtctc gctccggcag aaacagccga attaaccatt
     2761 ttgaaagctg tctgaacggt tcccagcata tcggtcgtcc gggcaataac atcgttctga
     2821 ataaagtccg gtattcccgc gagatcaaaa tcgccgaaca tgtcttcaat caactcatcc
     2881 aggaatacgg acgactcctc cagttttctg gcggttgcag ctccggcgac cggaaatgac
     2941 agttccccgc tctcaacaaa ctgaaatgaa acccggcaca tacggccttc agtgccggag
     3001 tgagaaacgg tcacctgccc gtcaatacag ccctgcattt caccgaactg cggatggatc
     3061 agcgtccccg gcccggcggt ttcaatcgct gtaatcagcc tgtcgcgctg ctctgcgtaa
     3121 tcatcaccaa cgagatacgc attaatcgtc agccgtcgcg tggcacgccc gagatcttcc
     3181 gtgtacggtt tgtcacggtt tgggtattca tggacctgaa cgcggcgtcc gaacgaaccc
     3241 tcgtcgctct ccaccgaaaa cggaacgcca cgaaatgaag cgtcgtaaag attatcgcgc
     3301 caattgatac ctgaagatgc agaaaagagt gaagaaagag aaggtaaaga aggtaagtcc
     3361 atactgtctc cactataaaa aaaccgccaa tttctggcgg cttatatatt aatcatcatt
     3421 aatatctttc atattcaatt tgcaattttc gtaggtcact cccgactcgc cattaacatt
     3481 aactccagct ctgatattcc cgttttcttt tacatctaca aaaaactggc ctcttggcat
     3541 cttgaacgca aatgtaaaac caacagtatc tgaatcaaaa tcatcatctt tatatattgg
     3601 gatcaaaaat gtcatcgtcg ctggtgatgc attgtttgca aaatcaaata cacctatact
     3661 tttgccgaat tcataagaag cttttctcaa tataatcgca ccgctggcag aaggggatgg
     3721 gcacgatatc ctcagttgtc tgaatccatc tgtatccaag tcaacgtcat tatctgcaga
     3781 tgttt
//
LOCUS       DAJVRF010000229         3750 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJVRF010000229 DAJVRF010000000
VERSION     DAJVRF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.232"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..377
                     /locus_tag="OQN13_003875"
     CDS             <1..377
                     /locus_tag="OQN13_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCS1617087.1"
                     /translation="MLPRDPLESLRVRKIEALADGIMDAGLVSVREQARPAAQQSEDE
                     LLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIACAVGYLNFRRVAPGWCVDRPH
                     LVKLVENLFSRESFARTEPPKA"
     gene            475..1866
                     /gene="selA"
                     /locus_tag="OQN13_003876"
     CDS             475..1866
                     /gene="selA"
                     /locus_tag="OQN13_003876"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCS1617088.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            1863..3707
                     /gene="selB"
                     /locus_tag="OQN13_003877"
     CDS             1863..3707
                     /gene="selB"
                     /locus_tag="OQN13_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCS1617089.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          826 a          958 c         1135 g          831 t
ORIGIN      
        1 cgatgttgcc gcgcgatccg ctggagtcgt tgcgggtgcg caaaattgag gcgctggcgg
       61 atggcattat ggatgccggg ctggtatcgg tgcgtgaaca ggcgcgtccg gcggcgcagc
      121 agtctgaaga tgaattgtta cgccagcggg agaaaatcaa ccgcagtctg gatgtgctgg
      181 aaggatatct ggtcgatggc acactcaaaa ccgatacggt caatctggcg actatcgcca
      241 ttgcctgtgc tgtcggatat ctcaatttcc gccgcgttgc gccaggttgg tgtgtcgacc
      301 gcccacactt agtcaaactg gtcgaaaacc tgtttagccg cgaaagtttt gctcgcaccg
      361 aaccgccaaa ggcttgatgc gcgatatgtc ctcctgaccc atctcacgtt acaatccgtg
      421 gttatgttaa acgcccttct ccgtgtgaga gggccttgat cagccaggtt tcctatgaca
      481 accgaaacgc gttccctcta tagtcaactt ccggctattg atcgcttatt gcgcgatagc
      541 tccttccttt ctttgcgtga tacttatggt cacacccgcg tggtggaatt gttgcgtcag
      601 atgctcgacg aagcgcgaga agtgattcgt gacagccaga cgcttcctgc gtggtgtgaa
      661 aactgggcgc aagaagtcga tgcccggttg acgaaagagg cgcagagcgc gctgcgtccg
      721 gtgatcaacc tgacgggaac cgtgctgcat accaaccttg gacgagcttt acaggcggaa
      781 gccgcggtgg aagccgttgc gcaggctatg cgttcgccag tgaccctcga gtacgatctg
      841 gacgacgccg gacgcggaca tcgcgatcgg gcgctggcgc aactgctgtg ccgtattacg
      901 ggggcggaag atgcctgtat cgtcaataac aatgcggcgg cggtgttatt gatgttggcg
      961 gccactgcca gcgggaaaga ggtggtagta tctcgcggcg aattggtgga gattggcggc
     1021 gcgtttcgta ttcccgatgt tatgcgtcag gcaggctgca ccctacacga agtagggacc
     1081 accaaccgca cgcacgcgaa tgattatcgt caggcggtga atgaaaatac cgcactgttg
     1141 atgaaagtac ataccagtaa ctacagcatt caggggttca ccaaagcgat agatgaagcg
     1201 gaactggtgg cgctcggcaa agagctggat gttcccgtag tgactgattt aggcagtggc
     1261 tcgctggtcg atcttagcca gtacggtttg ccgaaagagc caatgccgca ggagttgatt
     1321 gcggcgggcg tcagtctggt gagcttctcc ggcgacaagt tgttaggcgg gccgcaggca
     1381 gggattatcg ttggtaaaaa agagatgatc gcccgcctgc aaagccaccc gctgaagcgt
     1441 gcattacgcg cggataaaat gaccctcgcg gcgctggaag ccacgttgcg tctttattta
     1501 caccctgaag ctctgagtga aaaattaccg accctgcgcc tgcttacccg cagcgcagag
     1561 gtcattcaaa tccaggcaca acgtttacag gctccccttg ccgcacatta cggcgcggag
     1621 tttgcggtac aggttatgcc atgtctttcg cagattggca gtggttcgct gccggttgat
     1681 cgcctgccga gcgcggcatt aacgtttaca ccccatgatg gacgcggtag ccaccttgag
     1741 tcattagccg cccgctggcg tgaattgcca gtgccgatga ttggtcgtat ttatgacgga
     1801 cgattgtggc tggatttacg ctgccttgaa gatgagcaac ggtttttgga gatgttgttg
     1861 aaatgattat tgcgactgcc ggacacgttg accacggcaa aacaacctta ttgcaggtga
     1921 ttactggcgt aaatgctgac cgtctgccgg aagaaaaaaa gcgcggcatg accatcgatc
     1981 tcggctatgc ctactggccg cagccggatg gtcgcgtgcc tagttttatc gacgtacccg
     2041 gtcatgaaaa gtttctttcc aacatgctgg cgggcgttgg tggtatcgat cacgcgctgt
     2101 tggtggtggc gtgcgatgac ggcgtgatgg cgcagacccg tgagcatctg gcgattttgc
     2161 agctgaccgg taacccgatg ctgacagtgg cgctgaccaa agccgatcgc gtggacgaag
     2221 cgcgtgttga tgaggttgaa cgccaggtaa aggaggtgct gcgggaatac ggttttgctg
     2281 aggcaaaact gtttatcacc gcagcaaccg aaggtcgggg aattgatgcc ctgcgcgagc
     2341 atctgcttca gttaccagga cgtgaacacg ccagccaaca tagtgtccgc ctcgcgatcg
     2401 accgcgcatt taccgtaaaa ggtgccggtc tggtcgtcac cggtacggcg ttaagcgggg
     2461 aagtgaaggt aggcgattca ctctggctga ctggtgtaaa taaaccgatg cgtgtacgtg
     2521 cgctgcatgc acaaaaccag ccaacagaaa ccgcccatgc cgggcagcgt atcgcgctta
     2581 acatcgcggg tgatgcggaa aaagagcaga ttaaccgtgg cgactggctg cttgccgatg
     2641 cgccgccaga gccgttcaca cgggtgattg tcgagcttca aacccatacg ccgctgaccc
     2701 agtggcagcc gctgcatatt caccacgccg ccagccacgt tacgggacgc gtttcgctgc
     2761 tggaagataa ccttgccgag ctggtgttcg acacgccgct gtggctggcg gataacgatc
     2821 gcttagtgct gcgcgatatc tcggcccgca acacgctggc gggggcgcgt gtggtgatgc
     2881 ttaacccgcc gcgtcgcggt aaacgtaagc cggaatatct gcaatggctg gcgtctcttg
     2941 cacgggcgca gagcgatgcc gatgcgttat ctgttcatct ggaacgcggc gcggttaacc
     3001 ttgcggattt cgcctgggcg cgccagctca acggcgaagg gatgcgcgaa ttgctgcaac
     3061 agcctggcta tattcaggct ggttatagct tgttgaatgc gccggttgcc gcccgctggc
     3121 agcggaaaat tctcgacaca ttagcgactt atcatgagca acatcgcgat gaacctggtc
     3181 ctgggcgcga acgtctgcga cgtatggcgt tgccaatgga agatgaagcg ctggtactgt
     3241 tgctgattga aaagatgcgc gaaagcggcg acatcctcag tcatcacggt tggctgcatc
     3301 tgccggatca caaagcgggc ttcagcgaag agcagcaggc catctggcaa aaagcagagc
     3361 cgctgtttgg tgacgagccg tggtgggtgc gtgatctggc aaaagagacg ggtaccgacg
     3421 agcaggcaat gcgcctgacg ctacgccagg cggcgcagca aggaataatt accgcgatcg
     3481 tcaaagatcg ttattaccgt aacgatcgga ttgtcgaatt tgccaatatg atccgcgatc
     3541 tcgatcagga gtgtggttca acctgcgcgg cggatttccg cgatcgctta ggcgtaggcc
     3601 gaaagctggc aattcagatt ctggaatatt ttgaccgcat tggctttacg cgtcgtcgtg
     3661 gaaatgatca tttattgcgc gacgcattat tatttccgga aaaataagga aatgattaat
     3721 taatttttaa aataaattaa tacaaatttc
//
LOCUS       DAJVRF010000230         3745 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJVRF010000230 DAJVRF010000000
VERSION     DAJVRF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.233"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            387..1469
                     /locus_tag="OQN13_003878"
     CDS             387..1469
                     /locus_tag="OQN13_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCS1617090.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="OQN13_003879"
     CDS             complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="OQN13_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HCS1617091.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(2127..2267)
                     /locus_tag="OQN13_003880"
     CDS             complement(2127..2267)
                     /locus_tag="OQN13_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HCS1617092.1"
                     /translation="MMFEFYMAEFLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="OQN13_003881"
     CDS             complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="OQN13_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HCS1617093.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2623..2913)
                     /locus_tag="OQN13_003882"
     CDS             complement(2623..2913)
                     /locus_tag="OQN13_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCS1617094.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2906..3076)
                     /locus_tag="OQN13_003883"
     CDS             complement(2906..3076)
                     /locus_tag="OQN13_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617095.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(3076..3531)
                     /locus_tag="OQN13_003884"
     CDS             complement(3076..3531)
                     /locus_tag="OQN13_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HCS1617096.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
BASE COUNT         1023 a          885 c          813 g         1024 t
ORIGIN      
        1 ttttttacgt acgtaaacca ctaaatgata tttgcgagaa tgctaccgag tattgaaaac
       61 accactacaa atacataagc aaatcccaac aaataaccaa caaataattt ccagtgttat
      121 ttttagccgg tttaaattga accttcaaat tatagagcac ttataaataa cagccgttaa
      181 tataaattgg ctaatagatt tatttttatt cagtcaagag ccatgaatag gattcgatag
      241 aaaaaagttc agataaaaat agagatctac ttcacaaatc aaacgagaaa ccaaaactta
      301 catcttgaaa taatcacatt gattagatga atatttatcg cgcaatgaca tcatttttta
      361 ataatagttc aaaaaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag
      421 ctgcatcagt actgatggcg atgtctgctc aggcagctga aatttataat aaagacagta
      481 acaagctgga tctgtacggg aaagttaatg ctaagcacta cttctcctct aatgatgcag
      541 atgatggtga tactacttat gcccgtcttg gcttcaaagg tgaaacccaa atcaacgatc
      601 aactgactgg tttcggtcag tgggaatatg aattcaaagg caaccgcgct gaatctcaag
      661 gttcctccaa agataaaacc cgtcttgcct tcgctggcct gaaattcggt gactacggct
      721 ccatcgatta cggccgtaac tacggtgtag catacgacat cggtgcgtgg actgacgtcc
      781 tgccagaatt cggtggtgac acttggactc aaacagatgt gttcatgact ggtcgcacca
      841 ctggtgttgc aacttatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg
      901 ctgctcagta tcagggtaaa aatgaccgca ctgacgtaac tgaagctaat ggtgatggtt
      961 tcggtttctc cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat
     1021 ctgaccgcac taataatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa
     1081 atgctgaagt atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca
     1141 cctattctga aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac
     1201 aaaacttcga agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt
     1261 acctgcaatc taaaggaaaa gacttgggtg cgtggggtga tcaggacctg gttgaatata
     1321 ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa
     1381 tcaacctgat tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg
     1441 ttgctgtagg tatggtttac cagttctaat ttgattacta aaagatatgt tgcgggaggc
     1501 tttgcctccc caacatataa gtggctccct caagccactt cctttaggag cacaaccttg
     1561 cttctaacta tataaacctt ctgttatata ttacccttta tttttggggg cgttgcaacg
     1621 ccccattttt aataattttt agtaaacaat tggcatatta attagagtta ttaacaacga
     1681 tatccatctc taaccggata tctaatgcca ttaacatccc ttcaattatg ccctcagcct
     1741 tctgtaacct tttcccgata taaccatcag agcagcaatg cttacctgcc agtgacatga
     1801 atgtcatacc gactacataa taatctacta ataaatcgtg caaatcgctg ttgttctttt
     1861 tcagacgggc catgcacccg caaataatca tcgcgtcatc gtcacaacat tgcgggcgag
     1921 attttacttt tgaagtaatt aatcccttaa aaccggcggc aatggacgac caggtcacat
     1981 cttcatgatt attagccgcc cacgctcccc aacgctcaag aaccatctga atatcacgca
     2041 tcaacttact ccacaaaaat cagaccagaa cgccaattac aagcaaaaat caacaaaaca
     2101 gtattagttg attgttatct ctgacttcat actcctgctc ctgtcagggt tttggcgtaa
     2161 ttcttcagta ttcggtaatc ggtcaaaaca gaaccgggga aacgatataa gcgcagatgc
     2221 ccccagcggt ggcgaagaaa ttctgccata taaaactcaa acatcattca ttccccattt
     2281 cggtgatggt cagttccagc ctcccacctt tggtaacagg catcttcaca acgcggtaat
     2341 caacgacctg agcatcatcc agccagaaac ctgctttggt gagtgcgtca aaagcggctt
     2401 tttgcagatt atccaggtca cggcgacggc gatccggcat gtggcactca atacggattt
     2461 tcactggcat agccaggccg atatccagca ttgcgttttt aatgattcgg gtgacgttat
     2521 cgcggtatgc ctgcccttct gcgctgatgt gtgtgcgccc gcgattatgg cggtaatagc
     2581 ggttattgct cggaggccag ggtaatgtga tgctgtaggt attcacgcct taataacccc
     2641 ctctttcagc cagataacct gcgttctcgc cataccttcc agcgcgcatt cttttgcata
     2701 ctcagcatcg acaaaatatg tgcggcggtc gatttcgtcg tgacaggcag aacatgcaat
     2761 ggtggcaatc aggtctggcg gtttgatacc ggtaccgcac aatccagcca gccggatatg
     2821 tgccagtact gacgtttcag gattgccatt acatacgcca gggattctta cctggcattc
     2881 ccgaccacgc gctgcttttc tcaaatcagc catgattcct ccttgctgcc agtcgcaacc
     2941 attttttatc aaccaggcta gcggtatatc cgagcagtgt tggtatttcg gatggcttca
     3001 gctcaggctt acgcttacga cgatttgata ctctgtagat gtgcccgttc atgacacgaa
     3061 taagcggtgt agccattacg cctcctgctt atcgcgcagc tgctggtatt cacagctctg
     3121 cggaattgtc aggtggcagc cgatattcat cgcccaggct tcgactttgc acaggaagat
     3181 gtacatctcg ccggtttcca gctctgacgt atggcggagg gattggacgg tggtgacctc
     3241 gccggacacg acatctacac ggtctttgct ttcgtagccg agataggtgt gcttcatcgc
     3301 gtctttgacc cactcaggcg tagcgaaggt cttgccgcgg gcgatgagat attcgctgat
     3361 ctccgtgtac cacatgtggc tgagcgcgtt ctgcgacagg ctgcgcttct cgcgccacgg
     3421 cttaacctgc aggcgtaagc attgcccggc atccagcaat ggctgaatct gctggccgat
     3481 ggccgcgaag ttaccgcgat ggagtttgat gccgtctact ggcagagtca tacggcctcc
     3541 ctaacggaaa ccgcagaatg cagaaaatcg caggtgcatt tctgcatctg tgacaaggtg
     3601 atgagttcag attgtggtcg catttaagtc cccttaaatg cgcagaagtc accggagttg
     3661 ttcaggctcc gatgagagga ttatggcgga ttgattacga taaatcaaaa ctatcttgaa
     3721 gccaaaacag tgatcttcgc tttaa
//
LOCUS       DAJVRF010000231         3724 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJVRF010000231 DAJVRF010000000
VERSION     DAJVRF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.234"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            620..1336
                     /gene="nanC"
                     /locus_tag="OQN13_003885"
     CDS             620..1336
                     /gene="nanC"
                     /locus_tag="OQN13_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCS1617097.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1356..2462
                     /gene="nanM"
                     /locus_tag="OQN13_003886"
     CDS             1356..2462
                     /gene="nanM"
                     /locus_tag="OQN13_003886"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCS1617098.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2527..3507
                     /locus_tag="OQN13_003887"
     CDS             2527..3507
                     /locus_tag="OQN13_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCS1617099.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         1122 a          768 c          847 g          987 t
ORIGIN      
        1 tttagaccct aaataaattt tttataaaac aaacagttaa aagaaatcct ttagtttttg
       61 aagaccacct caagtgttcg tctggcttca catggcatct tcctcttaga aaaagatcga
      121 catattttgt gacacgaatt gcaaatctcg ttttgttgta tggcttgcgt gatttttgat
      181 ctggtataac aggtataaag gtgcaccgcg atagtcaatg agacagggca tctcgcaatc
      241 tatggcaaac atcacttcag ttctttctca tcgggtgatg aaaacgcact tcagtctgaa
      301 aggaatatga aaatgagatc aacagacact ctattttatg actctgggta aaatggattg
      361 agtaagtgat atagcttacg aacattcaaa tcaattaaac atcagaagag attttatact
      421 caggtattta atctgattct ctgtttattt aaataatgtg gaaagagaat tttcacagga
      481 gaccttatac aaaaatataa aataagctac ccgttgccaa agacactata agcctggcaa
      541 aaaatattac acaacataaa tgctaattgt ttatgcgggc tttgtattgc tttctgtatc
      601 ctacaaatga gtgaaattta tgaaaaaggc taaaatactt tctggcgtat tattactgtg
      661 cttttcgtca ccattaattt ctcaggctgc gacactggac gtacgtggtg gatatcgtag
      721 cggaagccat gcctatgaga ctcgactcaa agtcagtgag ggatggcaaa atggatggtg
      781 ggcaagcatg gaaagtaata cctggaatac cattcatgat aataaaaagg aaaatgccgc
      841 actcaatgat gttcaggttg aagttaatta cgcgattaaa cttgatgatc aatggacggt
      901 gcgcccggga atgttaacgc attttagcag caacggcaca cgctacggac cctacgtaaa
      961 actgtcctgg gacgcgacaa aagatcttaa ttttggcatt cgctatcgtt acgactggaa
     1021 agcttaccga caacaagact tatccggtga tatgtctcgt gataacgttc atcgttggga
     1081 tggatatgtc acttaccata ttaatagtga tttcaccttc gcatggcaaa cgacgctata
     1141 cagcaaacag aatgattatc gctatgcaaa ccacaagaaa tgggcgacgg aaaatgcctt
     1201 tgttctacaa taccatatga cgcccgatat tacgccatac atagaatatg actaccttga
     1261 ccgccagggt gtttacaacg gcagagataa tttatcggaa aacagttatc gcattggtgt
     1321 gtcatttaaa ctgtagtaga caggagacag tcacaatgaa taaaactata atggcgcttg
     1381 ctatcatgat ggcttcattt gccgcaaacg cgtctgtatt accggaaact cctgtgccat
     1441 ttaaaagtgg taccggagca attgataacg acactgtcta cattggttta ggtagcgcag
     1501 gtacagcatg gtacaagcta gatacacagg ccaaagataa aaaatggact gcgttagctg
     1561 cattccctgg tggaccgaga gatcaagcaa cctctgcatt tattgatggc aatctatatg
     1621 tgtttggcgg cattggcaaa aacagcgagg gattgactca ggtatttaat gacgtacaca
     1681 aatacaaccc caaaaccaat agctgggtta aattgatgtc gctcgcgccg atgggcatgg
     1741 cgggtcatgt gacttttgta cacaacggca aggcttatgt tactggcggt gttaaccaga
     1801 atatcttcaa tggctatttt gaagatctca acgaggctgg aaaagattca accgctatag
     1861 ataaaatcaa cgcccactat tttgacaaaa aagcagaaga ttatttcttc aataagtttc
     1921 tgttgtcttt tgatccctca acacagcaat ggagttacgc tggcgaatcg ccctggtacg
     1981 gaacggctgg tgcggcggtt gtgaataaag gtgataaaac ctggcttatt aatggcgaag
     2041 ccaaaccagg attgcgaacg gatgccgtat ttgaacttga tttcaccggt aataatttaa
     2101 aatggaataa gcttgatccc gtctcatcac cagatggcgt cgctggcggt tttgcgggga
     2161 taagcaatga ttctcttata tttgccggag gggccggatt caaaggttca cgagaaaatt
     2221 accagaacgg taagaactat gcgcatgaag gcctgaaaaa atcatatagc actgatattc
     2281 atctttggca taacgggaaa tgggataaat cgggtgaatt atcgcaaggt cgggcctacg
     2341 gagtatcatt gccctggaat aatagtttat taattattgg cggtgaaact gcaggcggca
     2401 aagcggtgac ggattcagtt ttaatttctg tgaaggataa taaagtcaca gtacaaaact
     2461 aacgcttcag ggccccggta aaggggccct gctatcaatt tgtcatttgt attacgcgaa
     2521 aattatatga acgcaataat atcgcccgat tattactatg ttcttaccgt tgctggtcag
     2581 tctaatgcca tggcgtatgg cgaaggactg ccattaccgg acagggaaga tgcgcctcat
     2641 tccagaatta aacaattagc gagatttgcg catacgcatc ccggaggccc gccatgtcac
     2701 tttaacgaca ttattccact gactcactgc ccacacgatg ttcaggatat gcagggttat
     2761 caccatcctc tggcaacgaa tcatcaaaca cagtacggca ccgttggcca ggcactgcat
     2821 attgcacgga aattactgcc cttcattcct aataatgcag ggattcttat cgttccgtgt
     2881 tgccgtggcg gatcggcttt taccgcgggc agcgaaggga catattcaga acggcacgga
     2941 gccagccatg atgcttgtcg ttggggaacg gatactccgc tgtaccagga tttagtcagc
     3001 agaacgcgag ccgcactggc aaaaaatccg cagaacaaat tcctcggcgt atgctggatg
     3061 caaggcgaat ttgacttaat gaccagtgac tacgcgtcac accctcaaca ctttaatcat
     3121 atggttgaag cctttcgtag ggatctaaaa caataccatt ctcagcttaa taatattact
     3181 gacgcgccat ggttttgcgg cgataccacc tggtactgga aagaaaattt cccgcattcg
     3241 tatgaagcta tttatggcaa ttatcaaaat aatgttttag ccaatattat tttcgtcgac
     3301 ttccagcaac aaggtgaaag aggactgacg aacgcgcctg atgaagaccc ggacgattta
     3361 agcacgggat attacggttc agcgtaccgg tcaccggaga actggacgac ggcactgcga
     3421 agcagtcatt tcagcacggc agcccgtcgg gggattattt ctgacagatt tgtagaagca
     3481 attttgcagt tttggcgcga aaggtgagcg gggagtttat tcatggggga tgtccttgtt
     3541 cgttctcaga gtagacaaca aaaaacaaat aagatcaagg taatatctct tcacttgcgg
     3601 gctcgaacag caggcaattg ccaatataat aatctggcac aagggtgaaa aacgcgcacc
     3661 gcataaaccg ttgttattgc ttacagatta gccggatgcc ttaatagaca tccgcgcctt
     3721 ttcg
//
LOCUS       DAJVRF010000232         3633 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJVRF010000232 DAJVRF010000000
VERSION     DAJVRF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.235"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(76..393)
                     /gene="hspQ"
                     /locus_tag="OQN13_003888"
     CDS             complement(76..393)
                     /gene="hspQ"
                     /locus_tag="OQN13_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCS1617100.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(451..1641)
                     /gene="rlmI"
                     /locus_tag="OQN13_003889"
                     /pseudo
     CDS             complement(451..1641)
                     /gene="rlmI"
                     /locus_tag="OQN13_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            1736..2014
                     /gene="yccX"
                     /locus_tag="OQN13_003890"
     CDS             1736..2014
                     /gene="yccX"
                     /locus_tag="OQN13_003890"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCS1617101.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(2011..2340)
                     /gene="tusE"
                     /locus_tag="OQN13_003891"
     CDS             complement(2011..2340)
                     /gene="tusE"
                     /locus_tag="OQN13_003891"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCS1617102.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2431..3090)
                     /gene="yccA"
                     /locus_tag="OQN13_003892"
     CDS             complement(2431..3090)
                     /gene="yccA"
                     /locus_tag="OQN13_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCS1617103.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(3297..3384)
                     /locus_tag="OQN13_003893"
     tRNA            complement(3297..3384)
                     /locus_tag="OQN13_003893"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3348..3350),aa:Ser,seq:tga)
BASE COUNT          981 a          933 c          876 g          843 t
ORIGIN      
        1 tgagatccct cgcctgggcc tggccaaata aaaaatcccc ggaaggcaaa aaccttccgg
       61 ggattcgttc agggatcagt tacgcagacg cggggcctgg agttgtttgc ggatggtctg
      121 cgccagctca tccatggatg gctgttcagg atgttcatcc tgcaattcgc tacttagctg
      181 ggcttcagcc aggtaggtat gaaccggtag gccgttatcg tcctccatca ctacgtgata
      241 ccacggagcg gcgcgaagct cgtcatttac cgccagctca tcaggcgacg gttcagagag
      301 cgaataaacc gggtcgatat ccacgaccac tccgagataa cctaacaggg aatggcggac
      361 ctgctggccg ataccgaatt tgctggcaat catagtcacc tcccgggaaa tctttacctt
      421 tgatataagg gtaaaattcc acatttcaag ttacatgacg cgacaggcaa accctttcag
      481 atatagccct tccggatagg tagcgatcac cggatgatcg gctgcctgac ggaactgctc
      541 tataaattgt acatcacggc cggcatcaat tgcggcatcc gcgatgattt tctgaaataa
      601 atcgctggtc atcagaccgg aacaggagaa agtcaggaga ataccgcctt cattcagcaa
      661 ctgaatcgcc agcatgttga tatctttata gccacgacac gcgcccatca actggctttt
      721 attctcaaca aacttcggcg ggtccatcac gataacgtca aatttttcac cgcgatcgcg
      781 ataagtacgc agcaatttaa agacatcatc acggacaaac tcagccttgc tcagatccag
      841 tttgttcagc tcaacgttct gccgtgcaat atccagcgct tcctaggagg tatcaacgct
      901 gacaacctgg ctgcaaccgc ccatcagtgc cgatacggcg aaaccaccgg tataggagaa
      961 acagttcagc acacgtttat tttcaacgta gcggcgggta gccaggcggc tgtctcgctg
     1021 gtccaggtag tagcccgttt tgtgtccgtg ctgaatatcc accagcagct tcatgccgtg
     1081 ttcttcaatc ggcagcaggg caggcggcaa ctcgccggtg acgaggccct gggtcagctc
     1141 catcccttct tttttacgta ccgcaacatc gctgcgatcg taaatcgcac attccgggta
     1201 cagcgtttgc agggcactaa ttaatgccgc gcgctgatat tctgccccag cactcagcag
     1261 ttgcagcacc agaaaattac cgaaacgatc gatagtaata cccggcaggc catcagattc
     1321 tccggcgatt aaacgatagc tgtcgaggcc atctttttgc gccagtcagt cacgccattt
     1381 ttgtgcttgt tgcaaacggc gggaaaaaaa agcaatgtcg atagactcag acgggtcaaa
     1441 cgtccagacg cgcgcccgga tttgcgaagc tggcgaataa gcgccgcgtg ctaaccattt
     1501 tccctgatga tcaacaatat cgatggtttc accgaggctg gctttacctt ccatgcgggc
     1561 aacggccccg gaaaagaccc acggatggcg acgaagtaat gatttttcgc gccctttggc
     1621 taacactaaa cgtacactca taatttactt ttctgtcgat gcaaagaaat gggcgtcatt
     1681 gtccggagtt ttccatggat ttgcaacgcg tccattaagg ataaggagag cagcaatgtc
     1741 gaaagtctgc ataattgcct gggtttacgg gcgagttcag ggcgtaggat ttcgctacac
     1801 cacacagtac gaagcgaaaa gactgggttt aaccgggtac gccaaaaatc ttgacgatgg
     1861 cagcgtggaa gtggttgcct gcggtgaaga agggcaggtg gaaaaattaa tgcagtggtt
     1921 aaagagtggc ggcccgcgtt ctgcgcgtgt agaacgggtg ctcagcgagc cgcatcatcc
     1981 ctcgggggaa ttaaccgatt ttcgtatccg ttaaatacat tttaccggtt ttggcaggcc
     2041 agcaattttg gtggcttgct tagccggacc tttcggaaac agtcggtata aatagcggct
     2101 gttacctttt tcttcgccaa atttattcgc catcgctttt accagcatac gaatcgccgg
     2161 agaagtattg aattccagat agaaatcacg cacaaaacgc accacttccc agtgttccgg
     2221 tgacagcgaa atcccttcgt tctctgcaat caccactgcc agcggctcac tccactggct
     2281 gctttctttg agatagcctt cggtatccgt ttctatctct ttaccttcga agatcagcat
     2341 aattattact accttaatca gactggcggc agtgtaacaa aaaacaaagc cccgcataaa
     2401 gcgaggctat gaaagtgtta gcgggtgaga ttaatcgcgg ctggcgaagc ccagaatgct
     2461 cagcaggctg acgaagatgt tgtacagcga aacatacagg ctaaccgtgg cacgaatata
     2521 gttcgtttca ccgccatgaa cgatgttgct ggtttcaaac aaaatagcgc cagaggagat
     2581 cagaatgaag accgcgctga tcgccagatg cagagcaggc agctgcagga agatattagc
     2641 aaccatacca atcagcacca ccacaatacc cgccatcagc ataccgccga ggaacgacat
     2701 atctttgcgg gtggtcagca catatgcaga gcagcagaag aacactaacg ccgttccgcc
     2761 cagtgccata gcgattacgt cacccattcc ggcagacaga taggtgttca gaataggtcc
     2821 gaggatataa cccagaaaac cggtaaaggc gaatgcggag ataatcccgg tcggcttatt
     2881 cgccgtttta taggtcagga acatcaaacc atacataccc accagcgtca gaatcagacc
     2941 cggagatggc agcatcagca ccgtactggc agttgcggta atcgccgaaa aggccagcgt
     3001 caggctcagc agaaaatagg tattacgcag caccttatgg gtgctaagca gtgatgtacg
     3061 gtcatgtgaa gaactaacaa tacgatccat tgagtcactc tctatgacag atgtaattaa
     3121 ttaagcagca taatgataat gcgtaagggc acccagaagt tttacccatc tttacgcatt
     3181 tgatctggaa caggtttaac agcggattat caggtcatta agcaaatata acgccctgag
     3241 aatttcgaca ggcaaaagaa aaaggggtta gcatttagct aaccccttat cttatttggc
     3301 ggaagcgcag agattcgaac tctggaaccc tttcgggtcg ccggttttca agaccggtgc
     3361 cttcaaccgc tcggccacac ttccggaatg acgcgcacta taaacatccc gatgcggcgt
     3421 gtaaacccct aatttgtttg tttgcctgaa aaacagccaa aagtgcattg atagcgtgaa
     3481 ataacagcag attgatcatt tcatcaccat gaattccttc tcttttactc gtttagcaac
     3541 cggctaaaca tccccaccgc ccggcaaaaa gaaaaatagg tccattttta tcgctaaaag
     3601 ataaatccac acagtttgta ttgttttgtg caa
//
LOCUS       DAJVRF010000233         3570 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJVRF010000233 DAJVRF010000000
VERSION     DAJVRF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.236"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OQN13_003894"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OQN13_003894"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCS1617104.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OQN13_003895"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OQN13_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCS1617105.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHFLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OQN13_003896"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OQN13_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCS1617106.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT          914 a          897 c          910 g          849 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcctggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agctaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccgatgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag gtgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtagc gcggaatggg catcagcaga
     1621 aacgtggcaa acaccaaatt gaggagcgga tggaagttaa gatatcccgc ccacaacagg
     1681 ccgaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aataaacgta
     1861 aggtgtcgcg aatgcgtcgc aaaaagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gacaataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcatgcccc attgttctgg
     2101 ggcaagcaag cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacacagcgt cagggcttgt tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggt aatagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggcacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tgttttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa
//
LOCUS       DAJVRF010000234         3561 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJVRF010000234 DAJVRF010000000
VERSION     DAJVRF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.237"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            250..507
                     /locus_tag="OQN13_003897"
     CDS             250..507
                     /locus_tag="OQN13_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HCS1617107.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(557..1507)
                     /gene="uspE"
                     /locus_tag="OQN13_003898"
     CDS             complement(557..1507)
                     /gene="uspE"
                     /locus_tag="OQN13_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCS1617108.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1659..2411)
                     /gene="fnr"
                     /locus_tag="OQN13_003899"
     CDS             complement(1659..2411)
                     /gene="fnr"
                     /locus_tag="OQN13_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCS1617109.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2606..3121)
                     /gene="ogt"
                     /locus_tag="OQN13_003900"
     CDS             complement(2606..3121)
                     /gene="ogt"
                     /locus_tag="OQN13_003900"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCS1617110.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3132..>3561)
                     /locus_tag="OQN13_003901"
     CDS             complement(3132..>3561)
                     /locus_tag="OQN13_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCS1617111.1"
                     /translation="KFIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWS
                     ILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLG
                     TYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT          939 a          850 c          863 g          909 t
ORIGIN      
        1 gatcattgtt ttatcaggct cctccagata attgtcgtca tgccggaaac ccctggcggg
       61 gctattttac cgcgacaatt cattcagatc atcaatagtc agggaaggaa gtagcaacat
      121 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      181 acaacggtga aatgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      241 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaaaaaatt tctctttggt
      301 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgactat cggcggcgtt
      361 atagaacagt acaatattcc gctgtctgag tggacgacat caatgtatgt gattcagtca
      421 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      481 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      541 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      601 ggtcaggttt aatgaccaac agatcgcagc gaagatgatc aatcacctgt tccgccgtgt
      661 tgccgaggaa tgctgctgaa ataccggtgc gtcctaccgt gcccagaacc acaatccccg
      721 cctgtaagtg ctccgccaaa tcaggaataa cctcttccgg cagacctttt tctacgtgcg
      781 tcatgttttc attaatgccg aatttctgcc gcagagcttt cattgccagc aaatgttgcc
      841 cacgaatggc atcgttataa acgctcggat caaattccgg cagttcaatc gcgatattaa
      901 ttggcgttac tgggtaagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      961 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
     1021 cgagattcac cgccaccagc gccttacctc cttccggcca cggctggtct ttcaccatcc
     1081 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1141 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgcccgccg ctgatcactt
     1201 cctgaatgat ggcttcgaag ggacggttat gccagaccac tttaatttca atgggaacgc
     1261 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1321 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1381 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1441 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1501 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1561 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1621 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacatt acgcgtatga
     1681 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1741 agcataccgc ttttctggaa gcgacctagc agacggctga tggtttctac cgtcaggccc
     1801 agatagttac cgatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1861 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1921 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1981 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacggg
     2041 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2101 atggcgtcaa atcccaccag gtcgccagct aaatggaaac cagtgatttg ctcgtcgcct
     2161 tgctcagtga tggtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2221 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2281 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2341 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2401 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2461 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2521 tatgtctatc tatttgaaaa cccttaagtt gttaagggta actttacata aaagtgtgaa
     2581 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2641 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2701 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2761 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgtccgcagg
     2821 ggatagtgcg tagagttttc cagacttcgc gctgaaatgg cgtcccccct gtggcagtgg
     2881 gaagcgtatc aataatgcta agattaccgg caaaataatc acgaagcttg tcgcttaaac
     2941 cgcctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     3001 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3061 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3121 tccgttctct cttaagacaa acgtgggtaa atccccggac ctatcggcag accaacaaga
     3181 taccacgcca gcaacatcag cagccatacc accaaaaaga taagcggata gggcaagact
     3241 aacgaatagt aagtacccag tttcgcgtct ggtttgtagc gttgcaggaa tccaagaaac
     3301 agtggaacaa aaggagatac cggcgctaaa ggcaatacgg atgagtcggc aatacgaaag
     3361 aggatttgcg caaatgccgg gtgaaagcca agtagcataa acattggtac gaaaatgggg
     3421 gccagaatcg accagattgc ggaaccgctg gcaataaaca tgcataagaa agaggaaagc
     3481 aacgccagac cgacaaacgc cgggatgccg ctaagccctg aactttccag tatatcggtc
     3541 agccccacgg cgatgaattt c
//
LOCUS       DAJVRF010000235         3525 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJVRF010000235 DAJVRF010000000
VERSION     DAJVRF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.238"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            116..511
                     /gene="caiF"
                     /locus_tag="OQN13_003902"
     CDS             116..511
                     /gene="caiF"
                     /locus_tag="OQN13_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCS1617112.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(720..1310)
                     /gene="caiE"
                     /locus_tag="OQN13_003903"
     CDS             complement(720..1310)
                     /gene="caiE"
                     /locus_tag="OQN13_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCS1617113.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1316..2101)
                     /gene="caiD"
                     /locus_tag="OQN13_003904"
     CDS             complement(1316..2101)
                     /gene="caiD"
                     /locus_tag="OQN13_003904"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCS1617114.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFDGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(2210..>3525)
                     /gene="caiC"
                     /locus_tag="OQN13_003905"
     CDS             complement(2210..>3525)
                     /gene="caiC"
                     /locus_tag="OQN13_003905"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCS1617115.1"
                     /translation="GLAKIGAIMVPINARLLREESAWILQNSQACLLVTSAQFYPMYQ
                     QIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQPATLCYAPPLSTDDTAEILFTS
                     GTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYLTVMPAFHIDCQCTAAMAAFSAG
                     ATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTLMAQPPSANDRQHRLREVMFYLN
                     LSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPGDKRRWPSIGRAGFCYEAEIRDD
                     HNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAKVLEADGWLHTGDTGYCDEEGFF
                     YFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIVVVGIKDSIRDEAIKAFVVLNEG
                     ETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCSGKIIRKNLK"
BASE COUNT          831 a          961 c          868 g          865 t
ORIGIN      
        1 cttatcgacg gtgaagttgc atactatcga tatatccaca attttaatat ggccttgttt
       61 aattgcttca aaacgagtca tagccagact tttaatttgt gaaactggag ttcgtatgtg
      121 tgaaggatat gttgaaaaac cactctactt gttaatcgcc gaatggatga tggctgaaaa
      181 tcggtgggtg atagcaagag agatctctat tcatttcgat attgaacaca gcaaggcggt
      241 taataccctg acttatattc tgtcggaagt cgcagaaata agctgcgcag ttaagatgat
      301 ccctaataag ctggaaggac ggggatgcca gtgccagcga ctggtaaaag tggtcgatat
      361 cgatgagcaa atttacgcgc gcctgcgcaa taacagccgg gataaattag tcggcgtaag
      421 aaagacgccg cgtattcctg ccgttccgct cacggaactt aaccgcgagc agaagtggca
      481 gatgatgttg tcaaagagta tgcgtcgtta atgtcatttc gttgatacca cgcagcctgg
      541 ttgctggatg caatgctggc gcatcttatc cagcctacgg ttgctctcca gccgggttgg
      601 ataaggcgtt tatgccgcat ccgacagcta tcgccggatg cgatgctggc gcatcttatc
      661 cggcctacgg ttgctctcca gcagagttgg ataaggcgtt tacgccgcat ccggcaagat
      721 caccgtttag gcgtcacatc cgtcgtcccc tgcaaacggg ggcgattttc ctccatttgc
      781 ctcagtggct gtgtttcatg taacgatgca tgacagcgcc cgacaagatc ctgatactct
      841 ttggtattca accgtttcca gtgtaactcg tcgtcactaa cactgcgtac agcgcgggct
      901 ggcgtaccca tcaacaactg gcgtttctcg ccgtgaaagc ctgctttgac aaagctcatg
      961 gcggcaacaa tgctctcttc gccaatgacc gcgccatcca taatcacgct gttcatcccg
     1021 accaatgcat cgcgaccaat cacacaacca tgcaggatcg ctccgtgccc gatatggccg
     1081 ttttccccaa cgatggtgtc agtgtcgcag tagccatgca taatgcagcc atcctgaata
     1141 ttggctcccg cttgcacgat caaccgcccg tagtcaccac gcagtgaggc gagtgggccg
     1201 atgtagacac cggctcccac aatcacatcg ccaatcaaga cggcactggg atggacaaac
     1261 gccgtcgggt gaaccaccgg aattaacccc tcaaaggcgt aatagctcac ggttgttaac
     1321 gtcctttcca caccggatcg cgcttctcgg caaacgccag cggcccttca atggcatcct
     1381 ccgaatgcag aaccgacgga tagtgtttca acacgccgct gcgaatatag cgatacgctt
     1441 cttctaccgg catttcgctg gtggtgcgga agatctcttt cagcgccgca atcgccagcg
     1501 gggcgctgtt aaccagctgc tgcgccagtt cgcgggcgtt atccatcagt tccgcctggc
     1561 taaccacgcg gttgactatc ccccaacgca gcgcctcttc tgcgcccatt cgtctgccgg
     1621 tcatcaccat ttcattgacg atggcaggcg gcaggatctt cggtagacgc agcacgccgc
     1681 cgctgtcagg aacgatgccc agcttggctt ccggcagggc gaagctggcg ttatcggcac
     1741 aaacaataaa atctgccgcc agcgccagtt cgaaaccgcc gtcaaaggca tagccgttca
     1801 cagctgcgat aaccggtttg tcgagattga aaatttcggt taatcccgca aatccacccg
     1861 ggccaaagtc agcatccggt gcttcgcctt ctgctgccgc ttttaaatcc cagcccgcgg
     1921 aaaagaactt ctctccggca ccggtaataa tggcgacacg taattgcgga tcgtcacgga
     1981 aatttagaaa tacttcgccc atttcaaagc tggtttttgc atcaatagca ttcgcttttg
     2041 gacgatcaag ggtaatttcc agaattgatc cattgcgggt cagatgtaat gattcactca
     2101 ttccttttct ccatttttgc tttttcaggg acgacaacat ccctgcaaaa aatgcatatt
     2161 gttttatagc gtgattatta gttggcaggg tagttccctg ctgtttcatt tatttcagat
     2221 tctttctaat tattttcccc gagcaattac gtggcagatc ttttctgatc tccagataag
     2281 agggcacttt aaatttcgcc atattttgtt cgcagaagcg gaaaaattcc tcttcgctca
     2341 atgtttcacc ttcattcagc accacaaatg ctttgatggc ttcatcgcga atcgaatctt
     2401 taatacccac aaccacgata tcctgaattt ttggatgggt ggcgatgata ttttccagct
     2461 ccacgcagga gacattctcg ccaccgcgtt tgatcatatt gcagcggcga tcgacgaaat
     2521 aaaaaaagcc ctcttcgtcg cagtatccgg tatcgccggt atgtagccag ccgtcggctt
     2581 ccagcacttt cgcagtggct ttcgggttga gaaaatactc tttgaagatg gttttccctg
     2641 gtacgccttt aatacagatt tcaccgatct cacctgccgg gagcgggcga ttgtgatcgt
     2701 cgcggatctc cgcttcgtag caaaaccccg cccgaccaat cgacggccag cgtcgtttat
     2761 cgccagggcg atcgccgata atgcccacaa tggtttccgt catcccataa gacgtcagca
     2821 agcgaacgcc gaagcgttca caaaacgcat ctttttcctg ctccgacaag ttgagataaa
     2881 acatcacttc ccgcaggcgg tgttgccgat cgttcgctga aggcggctgc gccatcaagg
     2941 tacggatcat catcggaata cattcggtaa cggtggcgcg gtacttctgt acctgtcccc
     3001 agaaggcgcg ggcgctgtat ttctcgacca gcacaaaggt ggccccggca gaaaacgccg
     3061 ccatcgccgc agtacactgg caatcgatat gaaacgcagg cattaccgtc aggtagacgt
     3121 catcgtcacg cagtgcacac tgccaggcgg agtaatatcc agcgaagcgc aggttgtaat
     3181 gggtaatcac cacacctttc ggtcgggagg tggtgccgga ggtgaagaga atttccgccg
     3241 tatcgtcagt cgatagcggc ggtgcatagc acaaggtggc aggttgttga tttttcagtt
     3301 gagtaaacga actcacgcca tcatcagcgg gaagtgccac atctgtcagg caaatgtgcc
     3361 gcaattgagt ggcatcttcc tgctgaatct gttgatacat aggatagaat tgcgcactgg
     3421 tcaccagcag gcacgcctgg ctattttgca ggatccacgc gctttcctcg cgcaacaggc
     3481 gggcgttaat cggcaccata atcgcgccaa tttttgccag cccga
//
LOCUS       DAJVRF010000236         3474 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJVRF010000236 DAJVRF010000000
VERSION     DAJVRF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.239"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            63..1226
                     /locus_tag="OQN13_003906"
     CDS             63..1226
                     /locus_tag="OQN13_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1617116.1"
                     /translation="MPNHDRFECQTAIDSDAILQLAWAVFKIFRIPVNQWTLATAALG
                     GVFLVSGLILLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFK
                     LDPVRYQARVDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKG
                     SQAAVNPFSERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQ
                     LTEAKYNLEQTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQN
                     SLLRLKPGDDAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVL
                     GTIELDPNDDIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1686..2123)
                     /locus_tag="OQN13_003907"
     CDS             complement(1686..2123)
                     /locus_tag="OQN13_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617117.1"
                     /translation="MADKAILWALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVNICSDDKNIKLRVNSTIMAIDDSIKCIDE"
     gene            complement(2212..>3474)
                     /locus_tag="OQN13_003908"
     CDS             complement(2212..>3474)
                     /locus_tag="OQN13_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HCS1617118.1"
                     /translation="HRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWM
                     SLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSL
                     ADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQMQNQ
                     MDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWCAEYDEWGNLLNEENPHQLQQLIR
                     LPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPITDIDPLGLA
                     TCLYSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNNMQCKNNPGCTHLQNRGPIP
                     QGVWSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLTQSCLNAFGPSLGPRFCSEG
                     CITGSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          865 a          811 c          808 g          990 t
ORIGIN      
        1 tcaatgatcg gaagtatgcc aaatcgtgat cgattgtgat gccaaactgt gatcggagat
       61 ggatgccaaa tcatgatcga ttcgaatgcc aaactgcgat cgattcagat gccattttgc
      121 agctggcgtg ggcggtcttt aaaatctttc gtattccggt gaatcagtgg acgctggcga
      181 cggcggcgct gggaggcgtg tttctggtga gtggtttgat tttgttgatg aactacaacc
      241 acccttacac ttttaccgcg caaaaggcag tgatagcgat ccctatcacg ccacaggtga
      301 cgggaattgt tactgaagtc actgacaaga ataatcagct tattcaaaag ggcgaggtgc
      361 tttttaagct cgacccggtt cgttaccagg cgcgagttga cagacttcag gctgacctga
      421 tgacggcgac gcataatata aagacgctgc gtgcgcagct cactgaagcg caggccaaca
      481 ccacccaggt ttcagcggag cgcgaccgtc tgtttaaaaa ttatcaacgt tacctgaaag
      541 gcagccaggc ggcggtgaat ccgttctcgg aacgtgacat cgacgatgcg cggcaaaatt
      601 tcctcgcgca ggatgcgctg gtgaaaggct cggtggcgga gcaggcgcag atccagagcc
      661 agctcgacag tatggttaac ggcgagcaat cgcagattgt gagcttaaga gcgcaactta
      721 ctgaagcaaa atataacctt gagcagactg tcattcgcgc gccgagcaat ggctacgtta
      781 ctcaggtact gatccgccca ggtacatacg cagctgcctt gccgctgcgt ccggtgatgg
      841 tcttcatccc cgagcaaaaa cggcaaattg tcgcccaatt tcggcaaaac tcgctgttac
      901 gtctgaaacc tggcgatgat gcggaagtgg tgtttaacgc gctacctggg caggtgttcc
      961 acggcaaatt gactagtatt ttacctgtcg tgccaggcgg ttcttatcag gcgcaggggg
     1021 tattgcaatc attaacggtc gttcccggca cggacggcgt gctgggaacc attgaactgg
     1081 accctaacga tgatatcgat gccttacccg acggcattta cgcccaggta gcagtttact
     1141 ccgaccattt cagccatgtt tcggtgatgc ggaaggtgct gctaagaatg accagttgga
     1201 tgcattattt gtatttggat cattgagggg gaagggggag attatggaac tttgctccct
     1261 gcaagggcta attatttttg catattactt cattggctgc gatgattttt ctttaatatt
     1321 cattatgtta ataagttctg gatgagtgtg ctctcattgg attttttatc cgtcttgttc
     1381 tgccgcagga tgagactata catcgtcgag atcgcaatga tgatatgagt tgttcctcct
     1441 gcgacggctc taatcaaaaa agagcaggag tgcttttcca tctattgcga tgatggcttt
     1501 attatctgaa ggatgacagt cacatttaaa aaatatttaa tctatagatc acaatgtagg
     1561 gtcatggaat atatgtatac aactattttc ttcttcactt ttaattatcg tattcattat
     1621 aaaactaagt taaaaaaact cctagagagt gagttattaa tatattttta aataaaatta
     1681 agttatcatt catcgatgca ttttatagaa tcatcaatgg ccataatagt actattaact
     1741 ctcaatttta tatttttatc atcactacaa atattaactg aatttatatc aatgattttt
     1801 ttattcttat cttttatttt atttactgtt tcaatgcaat cagcggcaag ttggagtgga
     1861 tttagatttt tcagatatgg tcttatgatc tttcccttac ttaataaata gcatgtatag
     1921 gattcggaaa gtccagcatc tattttgtac ctcattatat tagataagga tgtgagaaat
     1981 tccttgttat cattcgcagc catatatgcc agtccgagtt cagcctctgc ggccttacag
     2041 gcaaaatagc ttagtgaaca cgcttttcgt ccctctttag tactggctga tattagtgcc
     2101 cataatattg ctttgtctgc caaatctaac tctctctgaa attgtcttgc agtaactgca
     2161 ctcgcagaaa tagagaaaaa aatacaattg ttaataactt tgccatattt tttagtccgt
     2221 aactgttaat gtgctatctg gctctgaaaa tattaactca tttaattttt gcatgtcatt
     2281 agatgaacca gttatacaac cctcggaaca gaatcgaggt cctaaagacg gaccgaaagc
     2341 gtttaaacaa ctttgtgtta aaaatccatc acgattatat gtttctgtat ttgctgacgg
     2401 tactaaacgt ataccattag gttttctatt ggttgctcca ggaccattaa cattccagct
     2461 ccatacccct tgaggtattg gacctctatt ttgtaagtgt gtacaaccag gattattttt
     2521 acattgcata ttattaccat tattaccaga ggcgacagga atagtaagca catcataaga
     2581 gttatcatca ttttgcgtat ctgaaacgca tgaaagcata cttaaagtta tgctgtataa
     2641 acaggtcgct aatcccaacg ggtcaatatc agttatcgga ttcaacggat actgataaaa
     2701 attccatccc cccttcagtc caatcggatc ctgagtgata tatcgcccct gcagcgggtc
     2761 ataatagcgg tggcggttgt aatacaggcc ggactcctca tcatactgct gccccggcag
     2821 gcggataagc tgctgcagct gatgcgggtt ctcttcattc agcaggttgc cccactcatc
     2881 gtattctgcg caccattctg ttgccccttc cgtgctgata agcaccagcg gcaggccgcg
     2941 atggtcgcag tggtacaggt ggatttttcg cgccggcgtg tacaccgggt ccatctggtt
     3001 ttgcatctgc tccaccgtca ggccgcacga tgccagccag cggcggcttt cctcactcac
     3061 ccggtcagcc aggatttcac tttccagccg gtcgagcatc tgcaccagca ccggcgggaa
     3121 caccacactg ccaccgtctt cgccgccgga ctgctgaagg gcatccgcca ggctgcggcg
     3181 ctgcgttttc gccagctcac cggtggcagt ttcgaccctg atgagtggcg tgaagctccc
     3241 cggctgataa atcgtctgga tgcgggttct gtcgttctgt attgtggtca gccggtcgcc
     3301 gtcccagccg taccaggtca cttgcggttt ccgtgacagc gacatccagc ccgtcaggtc
     3361 ccgttcacgc cgccataccc gttttgccac cctgcggccc agcgggtcgt aaagatagcg
     3421 actttcgacc agcggctctg catattgtgt ccgcgtgtag tgcaccagcc ggtg
//
LOCUS       DAJVRF010000237         3397 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJVRF010000237 DAJVRF010000000
VERSION     DAJVRF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.240"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..399)
                     /gene="ydcO"
                     /locus_tag="OQN13_003909"
     CDS             complement(<1..399)
                     /gene="ydcO"
                     /locus_tag="OQN13_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HCS1617119.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRII"
     gene            452..988
                     /gene="sutR"
                     /locus_tag="OQN13_003910"
     CDS             452..988
                     /gene="sutR"
                     /locus_tag="OQN13_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HCS1617120.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1061..3022
                     /gene="rlhA"
                     /locus_tag="OQN13_003911"
     CDS             1061..3022
                     /gene="rlhA"
                     /locus_tag="OQN13_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCS1617121.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3114..3344)
                     /locus_tag="OQN13_003912"
     CDS             complement(3114..3344)
                     /locus_tag="OQN13_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HCS1617122.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
BASE COUNT          852 a          862 c          863 g          820 t
ORIGIN      
        1 aataatgcgc atcagacgag caaagagtcc cgttatgccg cagaggacta ttaacgcgtt
       61 ggtgacaata aaaacgccga tggcttcgtt aagtgttagt ccctgcaatc cggtgaccaa
      121 caaagccgcg ccaggcgttg accatgcggt gagaacaggt acgcgatacc ataatgtcag
      181 agtcagcgta ctgacgccca ttgccagccc cagcgccgtc atccagccag agatttgtgc
      241 agtggtggct ccggcgacaa tcgctgcttg ccagattatt gccgctgaac tggcgtagcc
      301 aattaatacc gccagaaacc ccgccagtag cgtgggtgga ggaatagaaa acagacgcat
      361 agtcacctcg tgcgttatag cgtacaggca ccgtagcatt tgtccgttat aacgcacaag
      421 tgataaactt ccgttttgcc ggaggagtcg catggaaaat ctcgctcgct ttttatccac
      481 tacacttaaa caactacgcc agcagcgcgg ctggagtctt tcgcgactgg cagaagcgac
      541 gggcgtttct aaagcaatgt tagggcagat tgagcgtaat gagtccagtc cgacggtcgc
      601 gacattatgg aaaattgcca ccgggttgaa cgtaccattt tcaacattta tttctccgcc
      661 gcagtccgct atgccttcag tttatgatcc acaacagcag gcgatggtga tcacttcgct
      721 gtttccttat gatccacaac tctgctttga acacttctcg attcagatgg catctggtgc
      781 aataagcgaa tcgacgcccc atgagaaggg ggtaatcgaa catgtcgtcg tcattgatgg
      841 acaacttgat ctgtgcgttg atggggaatg gcagaccctt aattgtgggg aaggcgttcg
      901 atttgctgca gacgtcacgc atatctatcg taatggtggg gagcaaaccg tacattttca
      961 ttccctcatc cattacccgc gcagttaagc gggaaaacta tttcgcaacg tcgcgcttgt
     1021 ggctaaaata gccgccattt ttcagctact ggataagaat gtgaccgtat cttctcatcg
     1081 acttgaactg ttaagcccgg cacgcgatgc cgccattgcc cgcgaagcta ttttgcacgg
     1141 tgccgatgct gtttatatcg gcggccctgg ttttggtgcc cgtcataatg ccagtaatag
     1201 cctgaaagat attgccgagc tggtgccgtt tgcccatcgt tatggtgcaa aaattttcgt
     1261 cacgcttaac accattttgc atgatgatga gctggaaccc gcgcaacggc tgattactga
     1321 cctctaccag accggtgtcg atgcgctgat tgttcaggat atggggattc tggaacttga
     1381 tattccgccg attgaactgc acgccagtac gcagtgcgac attcgtacag ttgaaaaagc
     1441 gaagttcctc tctgatgttg gcttcacgca gattgtgctg gcgcgagagc tgaatcttga
     1501 tcagatccgc gcgattcacc aggctacgga cgcgaccatt gaattcttta ttcatggggc
     1561 actgtgcgtg gcctattcgg gtcagtgcta catttctcat gcgcaaacag ggcgtagcgc
     1621 caaccgtggc gattgctcgc aggcgtgccg tttgccatac acattgaaag acgatcaggg
     1681 gcgggtggtt tcctatgaaa aacatctgct gtcgatgaaa gataacgatc agactgccaa
     1741 cctcggcgcg ctgattgatg ctggcgtacg ctccttcaag attgaagggc gttacaaaga
     1801 tatgagctac gtgaagaata tcaccgccca ttatcgccag atgctggatg ccattattga
     1861 agaacgtggc gatctgacgc gtgcttcatc aggtcgtact gaacatttct ttgttccatc
     1921 gacggaaaag actttccacc gtggtagcac agattatttt gtgaatgccc gtaaaggcga
     1981 tattggcgcg ttcgattcgc cgaaatttat cggcctgccg gtgggtgaag tattgaaagt
     2041 ggcgaaagat catctcaatg ttgccgtcac cgagccactg gcaaatggtg atggcctgaa
     2101 cgtgatgatt aaacgtgaag tcatcggttt tcgtgccaat acggtcgaga aaaccggaga
     2161 aaatcagtac cgcgtctggc ccaatgaaat gccagcagat ttgcacaaaa ttcgtccaca
     2221 tcacccacta aaccgtaatc ttgatcataa ctggcagcag gcactgacaa aaacctccag
     2281 cgaacgtcgg gtggcggtag acattgaact gggcggctgg caggaacaac tgattctgac
     2341 cctcaccagt gaagagggtg tcagcatcac gcatacgctg gacgggcagt tcgacgaagc
     2401 caataacgcc gaaaaagcaa tgaacaatct gaaggatggt ctggcaaaac tggggcaaac
     2461 cctctattac gcccgcgatg tgcaaattaa tttgccgggg gcgctgtttg taccaaacag
     2521 tctgttaaac cagttccgcc gtgaagctgc tgacatgctg gatgctgcgc gtcttgccag
     2581 ttaccagcgc ggcagccgta aaccggttgc tgatcctgcg ccggtttttc cgcaaacgca
     2641 tctgagtttc ctcgcgaacg tatacaacca gaaagcgcgt gaattttatc atcgctatgg
     2701 tgtgcagctg attgacgcgg cgtatgaggc acatgaagag aagggcgaag tcccggtgat
     2761 gatcaccaag cattgtctgc gctttgcctt taatctgtgc ccgaaacagg cgaaaggcaa
     2821 tatcaaaagc tggaaggcga cgccaatgca actggttaac ggcgatgaag tattaacgct
     2881 aaagtttgat tgccgcccat gcgagatgca cgtcattggc aaaatcaaaa atcacatact
     2941 gaaaatgccg ttaccgggaa gcgtagtggc atccgtaagt ccggatgagc tgctgaaaac
     3001 attgcctaag cgaaaagggt aaaacgccag ttttctggtt actcacaact cattgaatct
     3061 gcatgatatt gcctgccggg taaggcgtca cgccgcatcc ggcatcaaac gactcaacgt
     3121 tgtcccgctt ctggtttgcg cgatttttgc cagtaaaaat gctcgcgcaa accttccgcc
     3181 gactcttccg ccacagcacg caattcatcg ctgtccgctt catgacgcag ctgatgatcc
     3241 acattcttta cccacacaaa ttcatgtcct ttgtgccctg ccatgagttg tcctgagaac
     3301 agcgcacacg ttaataagac aaccgataac gccttcgtaa acatcctgcc acctttttct
     3361 taccttttgc cgctatgatg ccgatcgttt cttgagg
//
LOCUS       DAJVRF010000238         3377 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJVRF010000238 DAJVRF010000000
VERSION     DAJVRF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.241"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..628
                     /locus_tag="OQN13_003913"
     CDS             <1..628
                     /locus_tag="OQN13_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1617123.1"
                     /translation="IYTPLTLKLYDWWVLGVSNRLAWGCPTKEHLLPHFLEHLGNNHL
                     DIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHKISHDVFEPYPAALH
                     GQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDGVVHNSFGQKL
                     MRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTRVQGTVVMFSASGKK"
     gene            complement(683..781)
                     /locus_tag="OQN13_003914"
                     /pseudo
     CDS             complement(683..781)
                     /locus_tag="OQN13_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(827..1009)
                     /locus_tag="OQN13_003915"
     CDS             complement(827..1009)
                     /locus_tag="OQN13_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1617124.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGYEQIFEDKLSGTRTGRPG
                     LKRALKRLQKGDALVV"
     gene            1108..1998
                     /locus_tag="OQN13_003916"
                     /pseudo
     CDS             1108..1998
                     /locus_tag="OQN13_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            2001..2534
                     /locus_tag="OQN13_003917"
     CDS             2001..2534
                     /locus_tag="OQN13_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1617125.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(2563..3090)
                     /locus_tag="OQN13_003918"
     CDS             complement(2563..3090)
                     /locus_tag="OQN13_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1617126.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(3092..>3377)
                     /locus_tag="OQN13_003919"
     CDS             complement(3092..>3377)
                     /locus_tag="OQN13_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1617127.1"
                     /translation="ALGSGAQVASSGDIYGSVWENNWLSTWLHNHVVRDIRLGSIEYK
                     NVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYNVGSV"
BASE COUNT          951 a          750 c          734 g          942 t
ORIGIN      
        1 aatttacaca ccacttaccc taaagcttta tgactggtgg gttttgggag tatcaaatcg
       61 gcttgcatgg ggatgtccta caaaggaaca ccttcttcca cactttctgg aacatttagg
      121 taacaaccat ctggatattg gtgttggaac tgggttttac cttactcacg tacctgagag
      181 tagtctgata tctttaatgg atttgaacga agctagcctg aacgcggcat ctacaagggc
      241 tggggaatca aaaattaaac ataaaattag ccatgatgtt tttgaacctt atcccgcggc
      301 gttacatggt caatttgatt ccatttccat gttttacctt cttcactgcc tgcctggaaa
      361 tatatctaca aaaagctgtg taatacgcaa tgcggcgcag gccttaactg acgatggaac
      421 tctatacgga gccacaattc ttggcgatgg agttgtgcac aatagcttcg gtcaaaaact
      481 gatgcgcatt tacaatcaga aaggcatctt ttcaaacaca aaagattccg aagaaggctt
      541 aacacatata ctctcagagc atttcgagaa tgttaaaacc agggttcaag gtactgtagt
      601 aatgttttcc gcttcaggga aaaaatagca tccaaccgca gcacgttctt gcttaagacg
      661 tgctgcggca taatcccaat gattactccc tgacagggtt cgtaggccac tcaatatcag
      721 gtgcagttga tgtatcaaca cggttcagca acacccgata ctttttctag gcttccacca
      781 ccagcacgac aagatgccgc atacagtgcc ccagtcagtc cagttttcag acaaccagtg
      841 cgtcaccttt ttgaaggcgc tttaaagcac gttttaatcc aggtcggcct gtccttgttc
      901 cgcttaattt atcttcaaat atttgttcat atcctgcaca aacaagagcg tttcgttgca
      961 ggtctgtatt ctggtcattt gttgataccc ttacatagcc aatcagcacg ctgaatctcc
     1021 cgtccaaaag cacaaatcat gccatgcagg ccagaaaccg ccattatcta aaacctcggt
     1081 ttacgagaac tcggcacaag cggggagaaa ataccgttac tcagtacagc gaatacctgg
     1141 actaatcgac aaacattcag cggtggcctt tctggtgaac tatccggcaa tgcttctaca
     1201 gctgcaaaat taaaaactgc caggaaaata agcaatgtgg cttttgatgg ttcctccgat
     1261 atcacattaa aagcaagtca tgttggtgcg tttgccttag ggaaaacagg aagcaccgtt
     1321 gcgaatgata aagcagttgg atggaactgg agtagcggag cctataacgc aactattagt
     1381 ggtgcatcaa cgttaattat tcatttttat atgggagaag gaagttgtcc tgcagctcag
     1441 tttcggatta attataaaaa tggcggtatt ttttatcgtt cagcccgtga tggttatggt
     1501 tttgaagccg actggtccga attttacacc accagaaaac cttcagcagg agatgttggt
     1561 gcactgccgt tatctggtgg tcaactgaat ggtgcactgg gtatcggaac atccagtgct
     1621 cttggcggta attcgattgt attgggtgat aatgacacgg gctttaaaca aaaaggcgat
     1681 ggtaatctgg atgtttatgc taataacgtc catgttatgc gctttgtctc cggaagcatt
     1741 caaagtaata aaaccataaa tattacggga cgtgttaatc cctcggatta cggtaacttt
     1801 gattcccgct atgtgagaga tgtcagactt ggcacacgag ttgtttagac catgaagaaa
     1861 ggcgtgatgt atgaaaaatc aggccatgta atcacggggc ttggtattat cggtgaagtc
     1921 gatggtgatg atccggcagt attcagacca atacaaaagt taattaacgg aacatggtat
     1981 aacgtatcgc aggtataatc atgcagcatt taaaaaacat tgtcgcaggt aatccaaaaa
     2041 ccgttgaaca ataccagcta acaaagaatt ttgatgttat ctggttatgg tccgaagatg
     2101 gaaaaaattg gcatgaggaa ttaaaaaact ttcaggagga tacaataaaa ctggcttaca
     2161 cagttgaggg aataattatt gctatagaca aggatgtatc agcaattaat ccagaaggtt
     2221 taagtgtcgt tgagttgcct gatattacag caaatcgccg ggctgatatt tcgggcaact
     2281 ggatgttcaa agatggtgcg gtgataaagc gaacttatac cgaggaagag caaaggcaac
     2341 aagcggaaaa tgaaaagcag agcctgctgc agctcgtcag ggataaaacc cagctgtggg
     2401 actctcagct acagctgggc atcatttccg atgagaataa acaaaaatta accgagtgga
     2461 tgctctatgc gcagaaggtt gaatccacag acacctccag cctgccagta acgtttcccg
     2521 aacaaccaga atgagagaag gcccgctatc gggccttaat ttttattcag gcttttgtgg
     2581 ccattcagga tttgccgtat ccacacggct gaccagaaca ctgtagcgtt cccatgcttc
     2641 cagtcgtgtg cgttcctcgt ctgttgccat attcagcctg acagctcgtt ccagcggctg
     2701 gatgactgat tcagcttcgg aaagcaatgc ggccttttgt gattcggcct gttgttgcag
     2761 ttcctgttct gtatattccc tctttactac tttaccatcc agaaacatcc attttccgtt
     2821 attgtctgcg tgacgatttg ccgtaatatc aggaacttca actatgctta gcccttcagg
     2881 attaatagtt gaaacgtctt tactgacagc aacgatgatg ttgttctcat cgtaagcaat
     2941 ttttatggtg tctggctgaa aattcttttg ttcctcatac cagtttttgc catcttcgca
     3001 aaagagccat accacgcccg cttttttagt tagctggtat tgttccggtg ttttcggatt
     3061 cccggcctta atatttttca ggtgcatcat cttaaacact ccccacgttg taccatattc
     3121 ccccaatcaa tttctgaatt ggccttctgg caacagtgtc gacaatatca tccgcattgc
     3181 tgttaattgc ggctgttaaa acataacctg acgcatcgcc aaagccgtag tcgcgccata
     3241 cgtttttata ttcaatgctg ccaagacgaa tatcccgaac gacatgatta tgcagccatg
     3301 tgcttaacca gttgttttcc cacactgaac cataaatatc gccactagac gcaacttgcg
     3361 caccacttcc cagcgca
//
LOCUS       DAJVRF010000239         3210 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJVRF010000239 DAJVRF010000000
VERSION     DAJVRF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.243"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            6..500
                     /locus_tag="OQN13_003920"
     CDS             6..500
                     /locus_tag="OQN13_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1617128.1"
                     /translation="MAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVADPN
                     LDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGKGLA
                     KKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCEVRM
                     LREL"
     gene            complement(546..962)
                     /gene="rraB"
                     /locus_tag="OQN13_003921"
     CDS             complement(546..962)
                     /gene="rraB"
                     /locus_tag="OQN13_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCS1617129.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1124..2128
                     /gene="argF"
                     /locus_tag="OQN13_003922"
     CDS             1124..2128
                     /gene="argF"
                     /locus_tag="OQN13_003922"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCS1617130.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(2200..>3210)
                     /locus_tag="OQN13_003923"
     CDS             complement(2200..>3210)
                     /locus_tag="OQN13_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1617131.1"
                     /translation="DLLEKPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEID
                     DLNAVNNYLLNNKKATDNEINDIKVNLSHILIDSLDDAKVNLTPVIDSILETFLKSPY
                     INDVRILDWCFNKRMQYFGDSEKIKYACSVINHIDFSCDQSKDFSCDQSKIKIAETLF
                     FNLDKEPYKNSRKLQELIWDKLVAYVNDFSLSNQEKSRLILRLFDDVKLLFNEVPVSI
                     LVNDIFLKDFFMKQPDFAKWYFYQLLKCYEGEQLYLNELGYVYGDEEKTKEIVNKLPG
                     YVVKIFEEKMGNELKIRTRMMKILRNGKINIYGYINEEQLGKLNPPEDLRIAIKKLGW
                     NN"
BASE COUNT          888 a          719 c          674 g          929 t
ORIGIN      
        1 ataacattgc gccgcaatca cctgtaatgc gtcgcctgac gctgctggat aatcctgcta
       61 tcgcccgcgt cattcgtcag gtatccgccg aatacggtct taccgctgat aaaggctaca
      121 ccgtcgccga tccgaatctt gacgagctgt atcaagtgta tagtcagcct ggccatgcat
      181 attgggtggt tgagtacgag ggtgaagtgg tcggcggcgg tgggattgcg ccattaaccg
      241 ggagtgagtc ggacatttgc gaactgcaaa agatgtattt tcttccggct attcgcggta
      301 aagggctagc gaaaaaactg gccttaatgg cgatggagca ggcacgagag atgggcttca
      361 aacgctgcta tctggaaacg accgcttttt taaaggaagc cattgcgctt tatgagcatt
      421 tgggctttga gcatatcgac tatgcgcttg gctgcacggg ccatgtcgat tgtgaagtgc
      481 ggatgctacg cgaactctaa aaaaatgccc gctagtatgt gctatcgggc attttaatca
      541 attaattagt ggcgaacccc gtcatcgtct tcatcgacaa aatcttcatc gtcgccatct
      601 tcgccgttag gatcttcaaa gtaagtgccc caaccgtcgt actcaacgtc aaatttctcc
      661 gccagcgtca tcagttgttc aacctgggca tcgatcagat cggcattcaa cgcgcactcg
      721 ctgaggatgt cgcagcaaat cacgatatca ccatcttcaa cttccagctc ttctggatcg
      781 gtcacttcgt aaccgagttt aaacgcttca actgccgctt tttccagagt ttccagatcg
      841 tctgcggaaa gatggtgttc gatggtgtac aacgcgtccg gatcgctgcc atcttccagt
      901 aattcttcaa taatcaaacg tgtttcttca cgctgttctt ccagttgttc cgggtttgcc
      961 atggctcatt cctcttaatg tcctgccgat actcttattg tcacacacca ctgacattgc
     1021 ctccaccttt gtgataaaga tttatgcttt agacttgcaa atgaataatc atccatataa
     1081 attgaatttt aattcattaa ggcgttagcc acaggaggga cctatgtccg ggttttatca
     1141 taagcatttc ctgaaattac tcgatttcac gccagctgaa ctcaacagcc tgctgcagtt
     1201 agccgcgaag ctgaaagccg ataagaaaag cggtaaagaa gaagccaaac tcactggtaa
     1261 aaacatcgcg ctcatcttcg aaaaagactc gacccgtacc cgatgctctt tcgaagttgc
     1321 cgcatatgac cagggggctc gtgttactta tctcggccca agcggcagcc aaattggtca
     1381 taaagagtcg attaaagaca ctgcccgcgt gcttggtcgc atgtatgacg gtattcagta
     1441 tcgcggctat ggtcaggaga ttgtcgaaac actggcggaa tacgctggcg tgccggtatg
     1501 gaacggcctg accaatgagt ttcaccccac gcagctgctg gcggatctcc tcaccatgca
     1561 ggagcatttg cccggcaaaa cgttcaacga aatgacgcta gtctatgcag gtgacgcacg
     1621 taacaacatg ggcaattcga tgctcgaagc tgcagcgctt accggtctgg atttgcgtct
     1681 ggtcgcgcca caggcatgct ggccggaagc tgcgctggtt acggaatgcc gcgccctggc
     1741 acaacaaaat ggcgggaata ttacgctgac ggaagatgta gctaaaggag ttgaaggtgc
     1801 tgactttatc tataccgatg tatgggtgtc gatgggtgaa gcaaaagaga aatgggctga
     1861 acgcattgca ttgctgcgtg attatcaagt gaacagcaag atgatgcagt tgaccggtaa
     1921 cccggaggtc aaattcctcc actgcctgcc cgcgtttcat gacgaccaaa cgacgcttgg
     1981 caaaaaaatg gcggaagagt ttggcctaca tggcggaatg gaagtgactg atgaggtctt
     2041 cgaatctgcc gccagcattg tatttgatca ggcggaaaac cgcatgcata ccatcaaagc
     2101 ggtgatggtc gcgacgctca gtaaataatt gatatcgcta taaagatgcc gggaaataca
     2161 aaaacatccc ggcgtcacat tattttatgc agtgatagtt caattattcc accccaattt
     2221 ttttattgct atacgtaaat cttccggagg atttaatttt cctaactgtt cttcattaat
     2281 atatccatat atattgattt tgccattccg gaggattttc atcattcttg ttctaatttt
     2341 taattcatta cccatctttt cttcaaaaat cttaacgaca taacccggaa gtttatttac
     2401 aatttctttt gttttttctt catcaccata aacatatcct aactcattaa gatacagctg
     2461 ttcgccctca taacacttaa gtaattgata gaagtaccac ttagcaaaat caggttgctt
     2521 catgaaaaaa tccttcaaaa aaatatcatt tactaaaatg ctgacaggca cttcattaaa
     2581 tagtaactta acatcatcaa ataaccttag tattaagcgt gatttttctt gattgcttaa
     2641 gctaaaatca ttgacatatg caaccaactt atcccaaatc aattcctgta atttacggct
     2701 atttttatag ggttctttat cgagattgaa aaataaggtc tcagctattt taattttaga
     2761 ttgatcgcag ctaaaatctt tagattgatc gcagctaaaa tcaatatgat ttatcacgga
     2821 gcatgcatac tttatctttt cagaatcacc aaaatattgc attcttttat taaaacacca
     2881 atccagtatt cttacatcat taatatatgg ggattttaaa aaagtctcca gaatcgaatc
     2941 gatgaccggt gtaaggttaa cttttgcatc atctaaggaa tcaataagaa tatgagacag
     3001 gttaactttg atgtcattaa tttcattgtc agtagctttc ttattattaa gaagataatt
     3061 atttactgcg ttaaggtcat ctatttcttt cagtatccct ttttcaccaa gcaagtatga
     3121 aatcgattca ttacatacat aaggcattaa tagcagatgt tctttcctta ttgctaataa
     3181 tgcattgttc aatggttttt ctaataaatc
//
LOCUS       DAJVRF010000240         3189 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJVRF010000240 DAJVRF010000000
VERSION     DAJVRF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.244"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            413..1302
                     /locus_tag="OQN13_003924"
                     /pseudo
     CDS             413..1302
                     /locus_tag="OQN13_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1302..1592
                     /locus_tag="OQN13_003925"
     CDS             1302..1592
                     /locus_tag="OQN13_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617132.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            1913..2203
                     /locus_tag="OQN13_003926"
     CDS             1913..2203
                     /locus_tag="OQN13_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617133.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHIALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            2203..2622
                     /locus_tag="OQN13_003927"
     CDS             2203..2622
                     /locus_tag="OQN13_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617134.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYIKPLINKDN"
     gene            2790..3113
                     /locus_tag="OQN13_003928"
     CDS             2790..3113
                     /locus_tag="OQN13_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617135.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNVENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
BASE COUNT         1071 a          571 c          655 g          892 t
ORIGIN      
        1 cagaatgcct ctgagcacgt catacaggat tgctcaaagg ttgagctaaa attctcataa
       61 atatggtcat ttacaagaca ggtgattcag atgttaagaa gtatccctgt gaacaataaa
      121 tgaccaatca gtaatcattc tgtgaaataa ttgttgcaat ttataatcgc atctgttata
      181 gtttctatat gggctgataa aaaagttcat gtcttgaagt ggtaacactt ggggaggtgt
      241 cagatggttg gcgtatatta acctctcatt cagggtatac ctcagcgttt taatcatcgt
      301 actgtttcag gcatggactt ctttatcagt ccatcaatcc cccagatatt ttaatcacac
      361 aggctaatcg gttttatccg gttggccttt tcacgtatga ggtaatttat gaatgtctaa
      421 atataaccca aatcagtttt ataaatttga tgtgcgaatc tctcaacttt cagaagaaat
      481 taacgcagca gcaagattga tttatatggt catggtccat agctgtgact tctatgaaga
      541 gaaatacaaa tcatattcac caagtattga ccatctcgct tatatggctg gtgtatcaaa
      601 gagaacagca atcagagcca ttgagacact tgagaaacac ggtttaattc aggtgcaaaa
      661 gaatcagggg cgtcaaagca cttatcgtgt attcacactg gatgataagc cggaattact
      721 tgtaagacct acatcacagg aagttgcagc ctcagtaaga caaaatgaca ccactaccag
      781 tgacaaaatg tcatgggggt agtgacaaaa tgtcacccgt gttagagttt agataagtta
      841 ttagataaaa cattagataa taatattaca gataagaata aagagactgg acttaatgat
      901 gatattaaag aagatgactt tctttctttg gatgaattaa atgattttct ctctatgaat
      961 gaatctgacc ttgaagtaag ctctaaaaag acttctaaac aagacagaga aatcactgat
     1021 gtgagtgttg atagtgacca taaaaacaca cagtgccccg ccactgatga tgagttggac
     1081 cccttcgatg atgaagagga tgaacgtgaa aaagccatta agagggcaga gaagaaaaag
     1141 agattcgagc ttgcaagaca aaagaacaaa gtatcgggtt tagacccatt cgatgacagt
     1201 gaagatgatt attatctttc accgaaaaag aaacaaactc aggatgacaa gaagcaaaaa
     1261 attaaaccga agatgtattt tgccggtgga ggaattaagt aatgaaagaa tatcacggtg
     1321 aaaagagata caaagattac ctgttaaagc gttacagcat ttctcgtgaa ggtcatctta
     1381 tgaaggatac tcacggggaa gtttaccgga ttagaccaaa gaaagaaggg aggggttatt
     1441 tcttttacga tggtgtgaca gatttaaaga ttgacgcttt gaggtttgca gtgctgtatc
     1501 attttgatat ctggaattca atccaccagc tacgattaaa agacggtgac cccagcaact
     1561 taaaggccac caatattatc acaaaacgtt agtttgtttt accatcatca atcgtaattg
     1621 tgatttcata tttgaaaagc ttaatgctga aattgaatcg tgtacttctg gcgataatca
     1681 tttttaaaag ctgatacaca agaagcacca gaaacacaaa gcaacctaag cttgcgaatc
     1741 tgtgagattc agcaattgtt tcaagaaagc ccattaattc cccttattta tcaaagggaa
     1801 tgacctgtat cagcccccta caaccatatt aacatgtaaa tgagttgttg acaatatgtt
     1861 ataaatatgt taaagttagt tataagtcaa agattttaag aaggaaaatt taatgagaca
     1921 cacagttaca cgccttttgc cagaggcata ttccagactg gcagcaatca aagacaaata
     1981 cccagccatg agccacacag cacttgttgg tgagattttg aaggaacaca tagcccttac
     2041 accgtttgac gctatcaagg gcgttgcaga gggttctaag gccatcagaa tcacaacggg
     2101 ccaactggaa ctactcaaag cacagtccgc agtggcaggt tgctcaatga ttcaggtgtt
     2161 gagtgccaag gttcatcaac tggcggataa gctgggggtt taatgctatg cacatttgat
     2221 tatcatgcgt tttatcgcca tagaggtcac cttgtccgtg agaataacgg aacatggaaa
     2281 gatttaactc gtaatgctga acccttcaca caccagcaat taaaatcccg cttctccaga
     2341 tggaagaatg caccagcaga tactggtgat tttgagatta tccatacctt cgattatgcc
     2401 tcagaagatg accttcacga tgcaattaag ctgattgaag acttcaatac ctcagaagat
     2461 gtggatggtg acaaatataa atcccgtggt aaccgctata accgtgatga tatcactcag
     2521 gccataaatg ctggtcaatc tcagcgatac attacagaaa aatatggtat ttcacggaga
     2581 cagtatttct acattaagcc attgattaat aaagacaatt aaaaataacc atctaagaaa
     2641 cggtagaatg cactttacag gcagagttgc accttaattt gataaattac cttaccagcc
     2701 agtgcaactc cctctgtaac ccgcatggtt actggcctca gaatggctat tttaagaggt
     2761 ctgaggtgtg tttcatagga gatttaaaaa tgaataatta cccaatgcaa attatggttg
     2821 ataacgacac tgctatgatg gtgcaaagct tcattgatgc aggtatcgca attaattttg
     2881 ataaattact aaagctgatg gctgataatg tagaaaacat ctctgacttt attcagtcgg
     2941 tggagtttaa cgagcctcga atgatgctac caattacaga cagcaacatg aaaagattgg
     3001 ttatcgagca aacgaacata tacagtattt ccccagagca atttttgaaa ggtgctgtga
     3061 caatcctgta cagtgataat attctggtga cggattctgt gagggttcac taatgggttt
     3121 aataattgct tggacgttgc ttggcttatt tgttggttgt attattaaca atgcggtaaa
     3181 taagaattg
//
LOCUS       DAJVRF010000241         3174 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJVRF010000241 DAJVRF010000000
VERSION     DAJVRF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.245"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..626)
                     /gene="dsbA"
                     /locus_tag="OQN13_003929"
     CDS             complement(<1..626)
                     /gene="dsbA"
                     /locus_tag="OQN13_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCS1617136.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(643..1629)
                     /gene="srkA"
                     /locus_tag="OQN13_003930"
     CDS             complement(643..1629)
                     /gene="srkA"
                     /locus_tag="OQN13_003930"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCS1617137.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1706..1975)
                     /locus_tag="OQN13_003931"
     CDS             complement(1706..1975)
                     /locus_tag="OQN13_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCS1617138.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2045..2629
                     /gene="mobA"
                     /locus_tag="OQN13_003932"
     CDS             2045..2629
                     /gene="mobA"
                     /locus_tag="OQN13_003932"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCS1617139.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2611..3138
                     /gene="mobB"
                     /locus_tag="OQN13_003933"
     CDS             2611..3138
                     /gene="mobB"
                     /locus_tag="OQN13_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCS1617140.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          828 a          770 c          768 g          808 t
ORIGIN      
        1 tattttttct cggacagata tttcactgta tcagcatact gctgaacaaa aacatccata
       61 ttgctggtat ccataccctg cggattcagc tgatatttac cgttaacaaa catcgccgga
      121 acgccacgca attgcacgtc agctgcagct ttttcctgct gagcgaccag agatttgacc
      181 acgaagctgt tccacgccgc gtcgtactct tcacctttaa tacctgcgtt gataaataca
      241 tcgcggatat cagatgctga acgaatggtc tgggttttct gtacgccttc aaacagcgga
      301 acagtgactt tgtcttccac gcccagcgcc atcgccacag cccatgcctg agtcagatct
      361 ttgcccaggt caccacccat gaagttgacg tggtatttag tcatcttcac gccttccggc
      421 agttttttct tcacattatc agaaatatgc agaacttctt caaactgata gcagtgcgga
      481 cagaagaaag agaaaaactc cagcacttgc ggcgcgccag ctaccggttt ttccagggta
      541 gtgtactgtt taccatcttc atactgcgcc gccgatgcgc taaacgctaa aactaaacca
      601 gccagcgcca gccaaatctt tttcatgatc tactctctcc gattaataca taggtgttaa
      661 ttgtaaaggg ggttcttgta gaacttttgc ctgttctata aaagtcgccg tttgtcgcag
      721 ccagtaatct tccccggtta accacgggaa atttttcggg aacgcgggat cagcccaacg
      781 ccgcattagc caggcaagat aataaaccaa acgcatggcg cgtaaaggtt caatcagtcc
      841 gatttcagcg gtgtcgaact cgccaaattc ttcataagct tcaataatag tttccagttg
      901 catccgctgc tcggctttat cgccattgag caacatccac aaatcctgaa tagctggacc
      961 attacgtgca tcatccagat caacaaacat tggaccatcg cgccagagaa tattcccggc
     1021 gtggcagtct ccatgtagcc gcagaacggt gaaatcttcc cgccagtgtg ctgtaacggc
     1081 ggcgatcagc tcatctgtcg ctttcaggaa tgccgctttc aacccggaag gtatcagtgt
     1141 agcgtcctca aacagcttgc gtggctcaat gagatattca ttcagaccga tggtcgggcg
     1201 atggataaaa agctgtttgt gccccgtctg gtgcatacgc cctaaataac gcccaaccgc
     1261 ttccgtctga tcgatattat cagcttcgaa ctggcgacca ccgacgcttg gaaaaacagc
     1321 gaaataaaat ccctgatgat tcaataaagt ctgaccgtta aaggccacag gtgctgcgac
     1381 cggaacttca tcatttacca gctgcaacgc aaattgatgt tcttcgagga tttgatcggc
     1441 tgtccaacgt tcagggcgat aaaatttgac gacaaaacgt cgacgctctt cgtcctgaaa
     1501 ttgatagaca cggttttcat agctgttaag cggggtaaga ccggaatcca cccggatccc
     1561 ttgctcaaac agagcgtcca tgatggtatc cgggtgtagt gtctggaaag taaaagcgct
     1621 gttgttcatc ccatcatccg gaaaacatta cgaatgatga aggatatcat ttcacgatga
     1681 ttttggtggc gccgcttaca agcttttact ctttaattac gccgcgagcg cgtaacagcg
     1741 ccgttttgaa gtcttcttca taatctttct gcaaacccgg gatcaccgca tctttcgcgg
     1801 aatcacgcat tttcaggtga tagatcagaa tgtcatccgt caaatccgcc agttcgccgt
     1861 caaaacctga ctctttcgcc agtttctgca aaaattgcaa caggttaagg tctggctctt
     1921 tttgccaggc tggctggagg agttcaataa cttcattcag acgtttacat ttcatagtga
     1981 tgctccttac tcttgagaca gacacgttag cagggtcaat cccacaataa aagaggcgat
     2041 atcggtgaat ctgatgacga cgataacagg cgttgtgctg gcaggcggta aagccagacg
     2101 aatgggcggc gtagataaag gattgcttga attaaacggc aaaccattat ggcaacatgt
     2161 cgctgacgcg cttatgacgc agctctctca cgtcgtggtt aatgccaatc gtcatcagga
     2221 aatctatcag gcaagcggtc tgaaagtgat tgaagattca ctggcggatt acccaggccc
     2281 tctggcagga atgctttcag taatgcagca ggaagctggt gagtggtttt tgttttgtcc
     2341 gtgcgatacg ccttacattc cccatgtttt agcttcccgg cttaatcatc agcgcaaaga
     2401 tgcgcctgtc gtgtgggtcc atgacggtga acgcgatcac ccgactattg ctctggtaaa
     2461 ccgtgctatt gagcctttat tactagaata tctgcaagca ggagaacgcc gggtaatggc
     2521 atttatgcgt ctggctggcg gccatgcggt tgatttcagc gatcataaag atgcatttgt
     2581 taacgtgaat acgccagagg agcttgcccg atggcaggaa aaacgatgat accgttactc
     2641 gcctttgccg cgtggagtgg taccggaaaa actacgcttt tgaaaaaact gatcccggca
     2701 ttatgcgcca gagggatccg tccagggctg attaagcata cgcaccatga tatggatgtt
     2761 gataagccag gcaaagatag ctatgagctg cgcaaggctg gcgcggcaca aaccatcgtt
     2821 gccagccagc aacgatgggc cttgatgacg gaaaaaccag acgaagaaga gctggatcta
     2881 cagtttctcg caagtcgaat ggatacctca aagctggatt tgattctggt cgaagggttt
     2941 aagcatgaag agatcgcaaa gattgtgctg tttcgcgatg gagccggaca tcgacctgaa
     3001 gaattagtga tagacaggca tgttattgct gtagccagtg atgtgccgct taatcttgat
     3061 gtcgcattac tggatattaa tgatgttgag gggctggctg attttgtggt ggagtggatg
     3121 caaaagcaga acggataagc aagagtaggc tggataagac gcgccagtgt cgca
//
LOCUS       DAJVRF010000242         3147 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJVRF010000242 DAJVRF010000000
VERSION     DAJVRF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.246"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            43..1911
                     /locus_tag="OQN13_003934"
     CDS             43..1911
                     /locus_tag="OQN13_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HCS1617141.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            1948..>3147
                     /locus_tag="OQN13_003935"
     CDS             1948..>3147
                     /locus_tag="OQN13_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCS1617142.1"
                     /translation="MYLTDNIIKLSNKIGQRVYTVVSEMSIEAWITKEPQPFVYRRSG
                     AYQKLTIGSDWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGI
                     TNKASSYGVPPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIAT
                     RNQLLKSLYYDIEVLFDWINDGQKFESIHPKGITPEQFIAKRSERTDEIISTLEYIDN
                     TLITFSNEEIIKCQIAIQSIINKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRT
                     FATALANIEKYPDYIFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECD
                     LNLVSGESLIRQIMHGTRFTLQNFSKKINYVWQPDVFGFPATLPQILKKSGINYIASQ
                     KLSQNKIN"
BASE COUNT          998 a          573 c          614 g          962 t
ORIGIN      
        1 gtcttttata acgtctaaac ttttttatgc aaaggtgtat ttatgatttt ttttaacgat
       61 ataaagccca ctggttggtt aaagaataag atggaatggt atatgaacgg tcatatagga
      121 gaactggata aattagttcc ccatcttatt acggagcata aaatatatgg aagagatcgt
      181 ataactccac gcactgtatc tgctgaagat ggcgttatta aaaaacagaa taatcccgat
      241 gataacatga tgcaatatta ttggtgggat agtgaaactc aatcgaattg gaaggatgga
      301 tattgccgca gtgcctactt gttagataat aaagaatggc aggaaaaagc gagcgattta
      361 tgtttggaac ttatcgatac gcaagatgat gatggctaca ttggaattta tgattcatca
      421 ttaaaattta actgtaaagc agaaaacgga gagttctggg cacaagctac tgccttgcgt
      481 tttgtgcttg gatgttatga gtccacccgt aatgagagtt tattaacttc tcttacccaa
      541 gccgcacaat gcattatggc tggctaccct aaagaaacgt cacatccttt cgttgtggaa
      601 cagggttttg ctggacatag ccacggatta acaataacag atgttttcta ccgcctttat
      661 gaaataacca ataacattga atatctctca tattgcttgt ggctgtatga agattattcc
      721 gcaggatgcg tgtcggagca agatctgcaa tataaaaatg tcatagaccc tggttacctt
      781 cttaaaggtc atggcgtaca tacgtatgag catattaggg cattagccat cgcagcgagc
      841 caacggccgc agtatcaaac tgcattagat atttttttat cccagttgaa atattatctt
      901 acaccatctg gcgctcccat tggtgatgag tggattacag gacgcacagc taatgccact
      961 tatacaggct atgaatattg ttctgttcat gaactattta gcacttatgt tatgttaata
     1021 aaactgacca ctaatattgg tctggccgat gatgctgagt ggctattcta taatgctagt
     1081 tttggcatgc agcatcccat acttcacgcg ataatgtact gtaagactga taattgttat
     1141 gaagcaaatg aaaggaaaca tcctgacagt aatcagttta ataccagata taaatactcc
     1201 cctactcatg aggaggctgc tgtctgctgc gtaccgaata cagcaagaac cataccggct
     1261 tttgtcgaaa atatgtttca agaaaataac aatggtttta ctgcattatt ttatggcccg
     1321 tgtgagtttt atggcacatt taataatgtt gcaattaaaa ttacacaatt aactgaatac
     1381 cctcatgatt taagcgtaaa atgtctcatt gagcctgagt catctgttcg ttttgctctc
     1441 agtttccgtt accccggctg ggcaaaaatg atgattatta atggtgttac ttttactaaa
     1501 aatgatactc aaaactcact tattatttta gatagaacat ggcaacatca tgatgtgatt
     1561 gacattaaat ttatagctga tatacagttt aatactgact tatgtggtga cacttatatt
     1621 tccagaggtc ccgttttata tgcaattgag cttgagagcg atattttaat caaaaaaaac
     1681 ctgctgaacg ggaaatattt tgactcagga tacataccct gtagtagagc tgatgaatct
     1741 atccagttct cttatgctga tatggaatct ttttcatatt caacctcacc agagggcaaa
     1801 caatacattg aaggctgttt ttatttaaac aagactaaaa tagtcagaaa acttatacca
     1861 tttggtcaaa ccattcttcg caaagtaact ttctccaata tagagagtta atattttcga
     1921 gtgttataat taattaaagg gatttctatg taccttactg ataatattat caaactgtcg
     1981 aataaaatag gtcagcgtgt ttatactgtt gtctctgaaa tgtcgattga agcatggata
     2041 acaaaagaac cgcagccttt tgtttatcgc cgtagcggcg cttaccagaa actcaccatt
     2101 ggctcagact ggggtcaact gtttgactgt gcctggatgc gagtttatgg taaacgtcca
     2161 gcagataaat ttagacacaa actcgtcgct ctcgttgata tcggtggcga aggtcttatt
     2221 gtggataaga atggttcacc catctgcggc atcacgaata aagcatcatc ttatggcgta
     2281 ccgccagata aaccaggtaa atgggtggtt gacctttctc tcgttagtga aaatgacgag
     2341 gttgagtttt ggattgatgc tgcctgcaac gatttatttg gctatgttac taatggaggg
     2401 attattactg atgttcatat cgcgacacgt aatcaactac tgaaatccct ttattatgat
     2461 attgaggtgc ttttcgactg gattaatgat ggtcagaagt ttgaatccat acatcctaaa
     2521 ggtatcaccc cagaacaatt tattgccaaa agaagtgagc gtactgacga aatcataagt
     2581 actctcgaat atattgataa tacgttaatt acctttagca atgaagaaat aattaaatgt
     2641 caaattgcaa tccaatccat aatcaataaa aacaataatc cttcagagtt caggattatg
     2701 gctactggtc atgcccattt agatatagcg tggatgtggc ctttaagaga aggacgcaga
     2761 aaagcaataa gaacatttgc caccgccctt gcaaacattg aaaaatatcc tgattacatt
     2821 tttggcgcta gtcagtacca actttttcac tggataaaaa aagattatcc ctatttcttc
     2881 gaaaagttaa aaaaacaaat cgccgcaggc cgctttgagt tacaaggttg cttctgggtc
     2941 gaatgcgatt taaatctggt aagtggtgaa tcgctgataa ggcaaatcat gcatgggaca
     3001 cgattcacac tgcagaattt ttcaaaaaag ataaattatg tctggcaacc ggatgtcttt
     3061 ggattccccg caacgcttcc acaaattctc aaaaaatcag gcattaacta tattgcatcg
     3121 caaaagctct cacaaaacaa gattaat
//
LOCUS       DAJVRF010000243         3074 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJVRF010000243 DAJVRF010000000
VERSION     DAJVRF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.247"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(263..463)
                     /locus_tag="OQN13_003936"
     CDS             complement(263..463)
                     /locus_tag="OQN13_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617143.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
     gene            complement(463..735)
                     /locus_tag="OQN13_003937"
     CDS             complement(463..735)
                     /locus_tag="OQN13_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1617144.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(738..1016)
                     /locus_tag="OQN13_003938"
     CDS             complement(738..1016)
                     /locus_tag="OQN13_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617145.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(1017..1574)
                     /locus_tag="OQN13_003939"
     CDS             complement(1017..1574)
                     /locus_tag="OQN13_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617146.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPSKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
BASE COUNT          858 a          606 c          577 g         1033 t
ORIGIN      
        1 aattgataca ccggaatcag cgaaagcctg caccatcata gcagtgtcgc tatcaatgaa
       61 aatctgtaat tcatattcgt tcatttttaa aatctccttt ttaccagacc tcctcaatcg
      121 ctctgtaacg agctaaacag tctttttcag gggtagcctg tacagtggta ttacccaccc
      181 taaaataaga tagttttttg cgttagaatg cgattgagag cgtctgataa gctgtttttc
      241 taatttctct taaagtctcg gactaccaat aacctaattc cgctttattt cgttcaaacc
      301 attccgctgt aactgctgtc tttagtgttg cgtttgggct ggtggcatga atgaagatat
      361 cttttggatt gcaagcatca ccgaaaccac cttctttgat gagcaggtct gatacatgct
      421 taatcatttt atctgtcaaa tattcgaatt taatacataa cattaaatct ccaggagtaa
      481 attgtttggg tggcaatttc ttttattacc atccctgaag ataatctttt tagttgcctg
      541 aacttcatga tgcaaaaacc agatgacata cttcagataa taatcacgag tgccataacg
      601 aatcattgga ttaccgtaag catcatctct tgtcttaacc tctccaccat caatccattc
      661 aagcattcct gtctcataat taatcatgaa gtaagttgca aagaaccgtt taatatcatc
      721 tcgccattgc ttcataatca gacctcctga gacagtttgt gaattttgat acttagtaac
      781 cttgctaaat ccagtccaaa agcatttaag gatttcagac tatcaaggtc atcttgctta
      841 atcctgatat ggtgtcttgc agcctctaag cgactattaa gcaaatcctg ttcgttaata
      901 tcacggttaa ttgcaagctt catcactgct gagtgtggca gacctgtaat gtttttgatt
      961 cggttcagtt catcaaaggc tgtctttgta actctggtta atgtgtgttt attcatttaa
     1021 aaatcctctg taactaaata ggggtcaaac tcgaaatctg gttgctcgtt ttccattggc
     1081 tcaatattcc caacggcaat atgatgttca acaatgccaa ttaatttcgc gcacatcaca
     1141 gcaagcaaag cgcaatattt atcgcttggt aattcgtaac taagtctggt tgcctgtcgc
     1201 tgaagatgat ttgtcacggt ggatgcgtta atttcgtcaa gattctttag cagataaaat
     1261 agttttttgt tgtatttcgg gtggccttca cttctacaga acccaataaa aacatcatca
     1321 agcttgcttg gtaactgctc gtaactgtca aagttagcca gataattttt aatagtattc
     1381 tttgttgttt tgcgtactgg cataaatctg tctcgtggcg taatgctacg aatgaccgct
     1441 tcaatgttct tacgttcgga tttgctaaag gttgggactt cgtagctggt ggggttgttt
     1501 gtgtaagcgt aggagttgtg cttgttgcgt tctcttgatg cctgaagcat cgctctaaat
     1561 tctgaatact gcattcatgt tctgactctc ataagatgtt attattctgt taacctatat
     1621 acaatattag cattttcaaa gactaatgtc aagtattttt aacattttcg taacatctgt
     1681 ggtataatgg gtttatgggg agagtaaaat atgaggcgtg gctatgtatt tggtcgcata
     1741 ttttgcattg cacacagacc tcttcaatcg ccctgtaacg agttaaacga cctcttcagg
     1801 tcaactcata aggtcaggta ctttttgtgt cagaatgcga ttgagagcgt cttacaagga
     1861 aaactaatgg taaatctccc tcctgagctg gggtcacctg atgtatggac tggtggaggt
     1921 attgggctga ttacaggcct gctacttcgc tacgtcctcg tcagagtgct tgttgtcttt
     1981 aaactgttcg gtcgtacatg gacgcttgac cttaaggata acgaccacac agacccccgt
     2041 taaactcctt caaaacccct gcacgagatg acagatagaa gtctatggta caggggtgtc
     2101 tttagcattt tgtgctatct attaaaaaaa tagcttgaca gaaccagaaa gccgtatcta
     2161 tcaagcattc acctctctga cagtaatttc taaaagttct tatttttcaa taagttgtaa
     2221 ttagccatat atagagaact aaaagatttt ttagctattt tgtttaaaag catatcaaac
     2281 gatacatttt attgatttag gtcaaagaca aaagcattga caaaatggta agattgttca
     2341 catgacctta cttaacagac ttttaagaga ctttgaaaca gcctgtttaa caggtcacta
     2401 cctctggtct tatcgttccc ttctttctag gtggttatct gttaagagtt tttcagtttt
     2461 tattcttaag ataattgtta agatgatact ctcccttcgg gttatatcgt atctcttctc
     2521 ccgttaaata tcccttcaca gtcagactaa ataacacctg tgtagttctt ggtagaatct
     2581 ttcaaggtaa cttagctctg agtttatgaa gttgacctat gagatagact ctaccgaatc
     2641 tgttagtcaa ttgttgctta ggtgctgcta aggcttactt agtggaaaga gaggggatat
     2701 gtgaccatag gggaacccct cctgttaaaa gactacttag aagtctgagg ttttagtaaa
     2761 atctgtgaat atggcttctc agacgctctg taacgcattc taagcgattt taccccttta
     2821 ttttaaggtg gttagtacat ccctataggt tacccctaaa aatggggtta aaactcgcta
     2881 cagagctttc tatgagatta gcttctaatc gaatcaacta tgaggtttgg taggattgtg
     2941 caaaatctgt acagtaactt ttcagaatct gaaaattttt ataaaaaatg actagccctc
     3001 tttgaatctc atcttccccg aatcttcaaa aatatctgac agatgtctgt agctgctaag
     3061 tacccgtcac acca
//
LOCUS       DAJVRF010000244         3069 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJVRF010000244 DAJVRF010000000
VERSION     DAJVRF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.248"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            122..1576
                     /gene="amn"
                     /locus_tag="OQN13_003940"
     CDS             122..1576
                     /gene="amn"
                     /locus_tag="OQN13_003940"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCS1617147.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            1907..2623
                     /locus_tag="OQN13_003941"
     CDS             1907..2623
                     /locus_tag="OQN13_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1617148.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          873 a          672 c          657 g          867 t
ORIGIN      
        1 aagacagtca acgcgcttga tagcctggcg aagatcatcc gatcttcgcc ttacactttt
       61 gtttcacatt tctgtgacat actatcggat gtgcggtaat tgtatggaac aggagacaca
      121 catgaataat aagggctccg gtctgacccc agctcaggca ctggataaac tcgacgcgct
      181 gtatgagcaa tctgtagtcg cattacgcaa cgccattggc aactatatta caagtggcga
      241 attacctgat gaaaacgccc gcaaacaagg tctttttgtc tatccatcac tgaccgtaac
      301 ctgggacggt agcacaacca atccccccaa aacgcgcgca tttggtcgtt ttactcacgc
      361 aggcagctac accaccacga ttactcgccc tactctcttt cgttcgtatc ttaatgaaca
      421 acttacgttg ctgtatcagg attatggtgc gcatatctct gtgcaaccct cgcagcatga
      481 aatcccttat ccttatgtca tcgatggctc tgaattgaca cttgatcgct caatgagcgc
      541 tgggttaact cgctacttcc cgacaacaga actggcgcaa attggcgatg aaactgcaga
      601 cggcatttat catccaacgg aattctcccc gctatcgcat tttgatgcgc gccgcgtcga
      661 tttttccctc gcacggttgc gccattatac cggtacgcca gttgaacatt ttcagccgtt
      721 cgtcttgttt accaactaca cacgttatgt ggatgaattc gttcgttggg gatgcagcca
      781 gatcctcgat cctgatagtc cttacattgc cctttcttgt gctggcggga actggatcac
      841 cgccgaaacc gaagcgccag aagaagccat ttccgacctt gcatggaaaa aacaccagat
      901 gccagcatgg aatttaatta ccgccgatgc tcagggtatt actctggtga atattggcgt
      961 gggaccatca aatgctaaaa ccatctgcga tcatctggca gtgctacgcc cggatgtctg
     1021 gttgatgatt ggtcactgtg gcggattacg tgaaagtcag gccattggcg attatgtact
     1081 tgcacacgct tatttacgcg atgaccacgt tcttgatgcg gttctgccgc ccgatattcc
     1141 tattccgagc attgctgaag tgcaacgtgc gctttatgac gccaccaagc tggtgagcgg
     1201 caggcccggt gaggaagtca aacagcggct acgtactggt actgtggtaa ccacagatga
     1261 caggaactgg gaattacgtt actcagcttc tgcacttcgt tttaacttaa gccgggccgt
     1321 agcaattgat atggaaagtg caaccattgc cgcgcaagga tatcgtttcc gcgtgccata
     1381 cgggacacta ctgtgtgttt cagataaacc attacatggt gagattaaac ttcccggtca
     1441 ggctaaccgt ttttatgaag gcgctatttc cgaacatctg caaattggca ttcgggcgat
     1501 cgatttgctg cgcgcagaag gcgaccgact gcattcgcgt aaattacgaa cctttaatga
     1561 gccgccgttc cgataataag ttctgaacct tcttatcaga aatagatgtg aaggagtaag
     1621 taagacacct ggcaaatagc ctgcaataat cgtgggctgt ttgcttcctt gggcggatac
     1681 gagttttatt atcgtcttaa tgatttccac atattaaaag caagtatgct ttcaaaacac
     1741 aattataaaa aatcccgcca acaatataag tttttataaa attatggctt tagaatattt
     1801 ttatttctaa tagacgagat ttttcctgtt atgatataat atgccgaatt aacacatgtt
     1861 aacgatttac cagtaatgta aataaatttt cgaggagatc attccagtgg gacgtaaatg
     1921 ggccaatatt gttgctaaaa aaacggctaa agacggtgca acgtctaaaa tttatgcaaa
     1981 attcggtgta gaaatctatg ctgctgctaa acaaggtgaa cccgatccag aattaaacac
     2041 atctttaaaa ttcgttattg aacgtgcaaa gcaggcacaa gttccaaagc acgttattga
     2101 taaagcaatt gataaagcca aaggcggcgg agatgaaacg ttcgtgcagg gacgttatga
     2161 aggctttggt cctaatggct caatgattat cgccgagaca ttgacttcaa atgttaaccg
     2221 tacgattgct aacgttcgca caattttcaa taaaaaaggc ggcaatatcg gagcggcagg
     2281 ttctgtcagc tatatgtttg acaatgctgg tgtgattgta tttaaaggat cagaccctga
     2341 ccatattttt gaaattttac ttgaagctga agttgatgtt cgtgatgtga ctgaagaaga
     2401 aggtaacatt gttatttata ccgaacctac tgaccttcat aaaggaatcg cggctctaaa
     2461 agcagctgga atcactgagt tctcaacaac agaattagaa atgattgctc aatctgaagt
     2521 tgagctttcc ccagaagatt tagaaatctt tgaagggctt gttgatgccc ttgaagatga
     2581 cgacgatgta caaaaagttt atcataacgt cgcaaatctc taattatctt ttaaagaaat
     2641 ctgtctttac ggcagatttc tttaatctca tataattctt ataaaaaata taagattcaa
     2701 ctcgtcatat tgattatacc cccccgttcc cagagaaata atatttatta aaattccagt
     2761 tcttcttttt ctgattacag aaggcaaagt ggcaattacg catagtttcc cgataaagac
     2821 gcgatagcga catcccgcat aaggcatttt tctctttatc tttgtacggt acttcatgga
     2881 acagagtttt tgaccttgcg aatcgtgatg tctgttgggg aggggcaatt tgctcactga
     2941 agcgtgagac tcgattaagc gcacgaaaca cagaaatcaa aaaacccggt cactttttta
     3001 caaggtaacc gggtaaaaat aatttttatt ttttaactgt tttgagactc atagagatgt
     3061 ctcaaaact
//
LOCUS       DAJVRF010000245         3069 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJVRF010000245 DAJVRF010000000
VERSION     DAJVRF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.249"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..242)
                     /locus_tag="OQN13_003942"
     CDS             complement(<1..242)
                     /locus_tag="OQN13_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1617149.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="OQN13_003943"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="OQN13_003943"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCS1617150.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..2902
                     /gene="treF"
                     /locus_tag="OQN13_003944"
                     /pseudo
     CDS             2255..2902
                     /gene="treF"
                     /locus_tag="OQN13_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="cytoplasmic; catalyzes the hydrolysis of trehalose
                     to glucose; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
     gene            complement(2890..3069)
                     /gene="treF"
                     /locus_tag="OQN13_003945"
                     /pseudo
     CDS             complement(2890..3069)
                     /gene="treF"
                     /locus_tag="OQN13_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="cytoplasmic; catalyzes the hydrolysis of trehalose
                     to glucose; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
BASE COUNT          844 a          773 c          658 g          794 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtaacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccacc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 tagacgccga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaagatt tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gaagcgcggt ataacgcgtt ggcggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaaacctaa aaccttatta ttttcactga cagcggatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagtgagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc ccggacagta aaacctttcc cgactgcgca cctaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctac tggcgctgcc gcagtcatat attgtcccag gcggccgttt
     2761 tagcgaaacc tactattggg actcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca caacatggcg ataggcctga ttagggatca acgattctgc
     2941 accgtccatc cagaaggcat attccatttt aaggtggtcg agatagcggc gcgcaccgcg
     3001 tacaccatct tcttcaaaca actccaccat cagcgcaaaa accggtggtt gcgatcggct
     3061 caaataata
//
LOCUS       DAJVRF010000246         3055 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJVRF010000246 DAJVRF010000000
VERSION     DAJVRF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.250"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..324
                     /locus_tag="OQN13_003946"
     CDS             <1..324
                     /locus_tag="OQN13_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1617151.1"
                     /translation="FYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGKATGIYYS
                     AGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTRHHSLKEN
                     VAPGG"
     gene            551..1816
                     /locus_tag="OQN13_003947"
     CDS             551..1816
                     /locus_tag="OQN13_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1617152.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            1828..2694
                     /gene="ydiB"
                     /locus_tag="OQN13_003948"
     CDS             1828..2694
                     /gene="ydiB"
                     /locus_tag="OQN13_003948"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCS1617153.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            2725..>3055
                     /locus_tag="OQN13_003949"
     CDS             2725..>3055
                     /locus_tag="OQN13_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCS1617154.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLV"
BASE COUNT          769 a          671 c          744 g          871 t
ORIGIN      
        1 ttttatgtgg tgataatatt tgcttttgtc attggtttta cctctgctgg aggtgttgtg
       61 caaattggcc tgacgttaat ggctgaacgt ttcccttacg ctaaaggtaa agctacaggg
      121 atctattaca gtgcgggcag tattgcgacc tttactattc cgttgattac ggctcatctg
      181 tcccaaagaa gtattgccga tattatgtgg ttcgataccg ccatcgctgc catcggtttt
      241 ttactggcac tgtttatcgg cttacgcagc cgcaaaaaaa cgcggcatca ctcgctaaag
      301 gaaaatgtcg ctccgggtgg gtaatgcaat attcttttca ggtcatgcaa gatcttacgg
      361 ataaataact ctttctgcgc taactaagga aaatcgcgat caaaaacaaa ctatgacatg
      421 caatattcct ggaaacataa actttatgcc atgtacccag ggaaaatcat cttcagtata
      481 gtaattatgt aaaccgtcgg agaacaatac gtacggtaac gaaattatct ttcagcaagg
      541 agctgtgaaa atgtctcaaa ataaggcttt cagcacgcca tttatcctgg ctgttctttg
      601 tatttacttc agctatttcc tgcacggcat tagtgttatt acgcttgccc aaaatatgac
      661 atctctggca gaaaagtttt ccactgacaa cgcgggcatt gcctatttaa tttccggtat
      721 cggtttgggg cgattgatca gtattttatt cttcggtgtg atctccgata agtttggtcg
      781 tcgggcggtg atattaatgg cagtaataat gtatctgcta ttcttctttg gtattcccgc
      841 ttgcccgaat ttaactctcg cctacggtct ggcagtgtgc gtaggtatcg ctaactcagc
      901 gctggatacg ggtggctacc ccgcgctcat ggaatgcttt ccgaaagcct ctggttcggc
      961 ggtcatactg gttaaagcga tggtgtcatt tgggcaaatg ttctacccaa tgctggtgag
     1021 ctatatgctg ctcaataata tctggtacgg ctatgggctg attattccgg gtattctgtt
     1081 tgtactgatc acgctgatgc tgttgaaaag caaattcccc agccagttgg tggacgccag
     1141 cgtagctaat gaattaccgc aaatgaacag caaaccgtta gtctggctgg aaggtgtttc
     1201 atcggtactg ttcggtgtag ccgcattctc gaccttttat gtgattgtgg tgtggatgcc
     1261 caaatatgcg atggcttttg ctggtatgtc agaagctgag gcattaaaaa ccatctctta
     1321 ttacagtatg ggctcgttgg tctgtgtctt tatttttgcc gcactactga aaaaaatggt
     1381 ccggcccatc tgggctaatg tatttaactc tgcgctggca acaataacag ctgccattat
     1441 ctacctgtac ccttctccac tggtgtgtaa tgccggcgcc tttgttatcg gtttctcagc
     1501 agctggcggc attttacagc tcggtgtttc ggtcatgtca gagtttttcc ccaaaagcaa
     1561 agccaaagtc accagtattt atatgatgat gggtggactg gctaactttg ttattccgct
     1621 gattaccggt tatctgtcga acatcggcct gcaatatatc attgttctcg attttacttt
     1681 cgcgctgctg gccctgatta ccgcaattat tgtttttagc cgctattacc gcgtttttat
     1741 tattcctgag aatgatgtgc ggtttggcga acgcaaattt agcacccggt taaacacaat
     1801 taagcataga ggttaaagga gttaattatg gatgttaccg caaaatacga attgattggg
     1861 ttgatggctt atcctatccg ccacagttta tcgcctgaaa tgcagaataa agccttagaa
     1921 aaagcgggat tgccatttac ctatatggcc ttcgaagtgg ataacgatag ctttcccgga
     1981 gcaattgaag gattaaaagc cctcaaaatg cgcggaactg gtgtgtcgat gccgaacaaa
     2041 caactggcgt gtgaatatgt tgatgaatta acgccagcag ccaaactggt gggtgccatc
     2101 aacaccatcg ttaatgatga tggctatctg cgtggctata acaccgacgg cacgggccat
     2161 attcgcgcca ttaaagagag cggttttgat atcaaaggca aaacgatggt gctgttaggg
     2221 gccggaggtg cctcaacagc gattggcgcg cagggggcaa ttgaaggttt aaaagaaatt
     2281 aaactcttta accgtcggga tgagttcttc gataaagccc tcgccttcgc gcagcgggtt
     2341 aacgaaaaca ccgattgtgt cgtcacggtt accgatctcg ccgatcagca agcctttgct
     2401 gaagtcctgg cttccgccga cattttaacc aatggcacaa aagtaggtat gaaccccctt
     2461 gagaatgaat cattggttaa tgatatcagt ctgttacatc cgggactgct ggtcactgaa
     2521 tgcgtgtata acccgcatat gacgaagtta ttgcagcagg cacaacaagc aggttgcaaa
     2581 acgattgatg gatacggcat gttgttgtgg caaggggctg aacagttcac gttatggact
     2641 ggcaaagatt tccctctgga atatgttaaa caggtcatgg ggttcggtgc ctgacaggct
     2701 gaccgcgtgc agaaagggta aaaaatgaaa accgtaactg taaaagatct cgtcattggt
     2761 gcgggcgcac ctaaaatcat cgtctcgctg atggcgaaag atatcgcccg cgtgaaatcc
     2821 gaagctctcg cctatcgtga agcggacttt gatattctgg aatggcgtgt ggaccacttt
     2881 gccgacctct ccaatgtgga gtctgtcatg gcggcggcaa aaattctccg tgaaaccatg
     2941 ccagaaaaac cgctgctgtt taccttccgc agtgccaaag aaggcggcga gcaggcgatt
     3001 tccaccgagg cttatattgc tctcaatcgt gcagccatcg acagcggcct ggttg
//
LOCUS       DAJVRF010000247         3038 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJVRF010000247 DAJVRF010000000
VERSION     DAJVRF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.251"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..2722
                     /gene="lacZ"
                     /locus_tag="OQN13_003950"
     CDS             <1..2722
                     /gene="lacZ"
                     /locus_tag="OQN13_003950"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HCS1617155.1"
                     /translation="NPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGY
                     GQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPS
                     TQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFGGE
                     IIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFREV
                     RIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYP
                     NHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPHWLPAMSERVTRMVQRDRNHP
                     SVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDED
                     QPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFV
                     WDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQ
                     FFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIELP
                     ELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQLT
                     TSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSEAT
                     RIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYR
                     IDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAACF
                     DRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHR
                     HLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            2774..>3038
                     /locus_tag="OQN13_003951"
     CDS             2774..>3038
                     /locus_tag="OQN13_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1617156.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFA"
BASE COUNT          683 a          804 c          850 g          701 t
ORIGIN      
        1 gaatccgacg ggttgttact cgctcacatt taatattgat gaaagctggc tacaggaagg
       61 ccagacgcga attatttttg atggtgttaa ctcggcgttt catctgtggt gcaacgggcg
      121 ctgggtcggt tacggccagg acagccgttt gccgtctgaa tttgacctga gcgcattttt
      181 acgcgccgga aaaaaccgcc tcgcggtgat ggtgctgcgc tggagtgacg gtagttatct
      241 ggaagatcag gatatgtggc gaatgagcgg cattttccgt gacgtctcgt tgctgcacaa
      301 accgagcaca caaatcagcg atttccatgt tgccactcac tttaatgatg atttcagccg
      361 cgcggtactg gaggcagaag ttcagatgta cggcgagctg cgcgatgagc tgcgggtgac
      421 ggtttctttg tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga
      481 aattatcgat gagcgtggtg gttatgccga tcgcgtcacg ctacgtctga acgtcgaaaa
      541 cccggcgctg tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac
      601 cgacgacggc acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat
      661 tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg attcgcggcg ttaaccgtca
      721 cgagcatcat cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct
      781 gctgatgaag cagaacaact ttaacgccgt gcgttgttcg cattatccga accatccgct
      841 gtggtacacg ctgtgcgacc gcttcggctt gtacgtggtg gatgaagcca atattgaaac
      901 ccacggcatg gtgccaatga atcgtctgac cgatgatccg cactggttac ccgcgatgag
      961 cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc
     1021 gctggggaat gagtcaggcc acggcgctaa tcacgacgca ctctatcgct ggatcaaatc
     1081 tgtcgatcca tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga
     1141 tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa
     1201 atggtccatc aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga
     1261 atacgcccac gcgatgggta acagtcttgg cggcttcgct aaatactggc aggcgtttcg
     1321 tcagtacccc cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa
     1381 atatgatgaa aatggcaatc cgtggtcggc ttacggcggt gattttggcg atacaccgaa
     1441 cgatcgccag ttctgcatga acggtctggt ctttgccgac cgcacgccgc atccagcgct
     1501 gacagaagca aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga
     1561 agtgaccagc gaatacctgt tccgtcatag cgataacgag cttctgcact ggtcggtggc
     1621 gttggatggc aagccgctgg caagcggtga aatgcctctg gatgttgctc cacaagataa
     1681 acagttgatt gaactgcctg aactaccgca gccggagagc gccggacaac tctggcttac
     1741 ggtacacgta gtgcaaccga acgcgaccgc atggtcagaa gccggacaca tcagcgcctg
     1801 gcagcagtgg cgtctggcgg aaaacctcag cgtgacactc cccgccgcgc cccacgccat
     1861 cccgcaactg accaccagcg aaacggattt ttgcatcgag ctgggtaata aacgttggca
     1921 atttaaccgc cagtcaggct ttctttcaca gatgtggatt ggcgctgaaa aacaactgct
     1981 gacgccgctg cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga
     2041 agcgacccgc attgacccga acgcctgggt cgaacgctgg aaggcggcgg gccattacca
     2101 ggccgaagca gcgttgttgc agtgcacggc agatacactt gccgacgcgg tgctgattac
     2161 gaccgctcac gcgtggcagc atcagggaaa aaccttattt atcagccgga aaacctaccg
     2221 gattgatggt agtggtcaaa tggcgattac cgttgatgtt gaagtagcga gcgatacacc
     2281 gcatccggct cggattggcc tgacctgcca gctggcgcag gtagcagaac gggtaaactg
     2341 gctcggatta gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg
     2401 ctgggatctg ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct
     2461 gcgctgcggg acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt
     2521 caatatcagt cgctacagcc aacaacaact gatggaaacc agccatcgcc atctgctgca
     2581 cgcggaagaa ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga
     2641 ctcctggagc ccgtcagttt cagcggaatt ccagcttagc gccggtagct accattacca
     2701 gttggtctgg tgtcaaaaat aataataacc gggcaggcca tgtctgcccg tatttcgcgt
     2761 aaggaaatcc attatgtact atttaaaaaa cacaaacttt tggatgttcg gtttattctt
     2821 tttcttttac ttttttatca tgggggccta cttcccgttt ttcccgattt ggctacatga
     2881 catcaaccat atcagcaaaa gtgatacggg tattattttt gccgctattt ctctgttctc
     2941 gctattattc caaccgctgt ttggtctgct ttctgacaaa ctcgggctgc gcaaatacct
     3001 gctgtggatt attaccggca tgttagtgat gtttgcgc
//
LOCUS       DAJVRF010000248         2994 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJVRF010000248 DAJVRF010000000
VERSION     DAJVRF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.252"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            18..1076
                     /locus_tag="OQN13_003952"
     CDS             18..1076
                     /locus_tag="OQN13_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCS1617157.1"
                     /translation="MAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTV
                     FTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACH
                     SLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFR
                     DHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKS
                     SEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDH
                     LVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKK
                     SFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="OQN13_003953"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="OQN13_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCS1617158.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2994
                     /locus_tag="OQN13_003954"
     CDS             2805..>2994
                     /locus_tag="OQN13_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylanase"
                     /protein_id="HCS1617159.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSL"
BASE COUNT          839 a          632 c          745 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc ttag
//
LOCUS       DAJVRF010000249         2944 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJVRF010000249 DAJVRF010000000
VERSION     DAJVRF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.253"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            45..1120
                     /locus_tag="OQN13_003955"
                     /pseudo
     CDS             45..1120
                     /locus_tag="OQN13_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            1136..1513
                     /locus_tag="OQN13_003956"
     CDS             1136..1513
                     /locus_tag="OQN13_003956"
                     /inference="COORDINATES: protein motif:HMM:NF012565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS1617160.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKM"
     gene            1513..1884
                     /locus_tag="OQN13_003957"
     CDS             1513..1884
                     /locus_tag="OQN13_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS1617161.1"
                     /translation="MQVTMQHALKLFWRPKAIELEDDGVMSYEKVEIIRRNDGSIRFN
                     NKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVGYINDFGGLSF
                     YEINCPTVNNSCNVSVAKRDK"
     gene            1886..>2944
                     /locus_tag="OQN13_003958"
     CDS             1886..>2944
                     /locus_tag="OQN13_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617162.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT          932 a          587 c          613 g          812 t
ORIGIN      
        1 gggcaactgg acgtcaaggt tgaagaagag gacgggccag tctatatatc aatgacacta
       61 accattacat gaattatcag aatgaaaaag agagttatat tgtcacgttt aaccagtatc
      121 ttgaatcatt aaggttaaat acatacgtaa gtttggctcg taatacttac tgggacgcca
      181 gcagtaatgt gaattattca ttatcactta gccgcgattt tgatatcggg ccattaaaaa
      241 acgtctccgc ttctctaaca tttagccgaa taaactggga agaagacaac caggatcaac
      301 tgtacctaaa tatttcgatt ccctggggaa ccagtagaac attgagctat ggtatgcaac
      361 gaaatcagga taacaagatt tcgcatactg cttcgtggta tgactcttcc gatcgaaata
      421 attcctggag cgtttctgct tcaggcgaca atgatgaatt caaagatatg aaagcgtcac
      481 tacgcgccag ttatcagcat aataccgaga acggtcgtct ctatctctcc gggacatcac
      541 agcgagacag ttattattct ctgaatgcca gttggaatgg ttcattcacg gcgactcgtc
      601 acggagccgc tttccatgac tatagtggta gtgctgactc gcgttttatg atcgacgcag
      661 atggtgcaga agatattccg ttgaacaata aacgcgcggt aactaatcgg tatggcatcg
      721 gagttattcc atcagtcagc agttacataa caacatcatt aagcgttgat acccgaaatc
      781 ttccagaaaa tgtggatatc gaaaactcgg ttatcaccac caccttaacc gaaggtgcta
      841 ttggctacgc caaacttgat accccaaggg ctatcaaatc atgggggtta ttcgcctggc
      901 agatggtagt cacccaccac tggggattag cgtaaaagat aaaaccagcc ataaagaatt
      961 aggactggtt gctgatggcg gctttgtata cctcaacggc attcaggatg acagcaaact
     1021 tactttacgc tggggtgata aatcctgttt tattcaacca cccaatagca gcaacttaac
     1081 caccggaacg gttattttac cgtgtattag ccaaaattaa ttcaggatga aactaatgcg
     1141 ctacttaaac acaaagaatc tcattgcagc aggagtatta ctcgcctgca tgagcagcat
     1201 tgcctggggt gccatcatcc cggaccgtac ccgtatcata atgaatgagt cagataaagg
     1261 ggaagcatta aaacttacta atcaaagcaa aaatctaccc tatctggcgc aaacatggat
     1321 agaagatacg aaaggcaata aatcacgtga ctttattgtc acggttcccc ctatggtacg
     1381 tttaaatccc agtgagcaaa ttcaaataag aatgataagt caggaaaaaa ttgctcaact
     1441 gcctaatgac agagaaacgc tcttctattt taacgtgcga gaaataccgc caaaaacaga
     1501 taaaaaaatg tgatgcaagt cactatgcag cacgcattga aactattctg gcgaccaaaa
     1561 gctattgaat tagaagatga tggggtaatg tcttacgaaa aagttgaaat catccgtaga
     1621 aatgatggtt cgatccgctt taacaataag atgccttatc acgttacgct gggttatatc
     1681 ggaacaaacg gaatcacaat gttgccacaa acgcaaagtt taatggtaac cccttatagc
     1741 catgcagata cgcagtttaa aaatgttccg tcagcttttc aggttggtta tatcaatgat
     1801 tttggtggat taagttttta tgaaataaac tgcccgacgg ttaataactc ttgtaatgtt
     1861 tccgtagcca aacgagataa ataatatgcg ctacttatta attgtaatta tatttattat
     1921 aggttttagt gcattacctg tatgggcaat ggactgctat gctgaacatg aagggggaaa
     1981 taccgtagtc attggttacg taccaagaat ttctatcccc agtaatggta aaaaaggtga
     2041 taagatctgg caaagcagtg agtattttat gaatgtcttc tgtaataatg cattacctgc
     2101 cccatcacct ggagaagaat acccatcagc ctggactaat ataatgatgt ttttaccagg
     2161 cggtcaggac ttttataatc agaactccta tatttttggc gtaacataca atggagttga
     2221 ttatgattcg actgccccta atgctatcgc agcaccagaa tgcattgata taaaaggagc
     2281 gggtaccttt aataatcact ataaaaaccc cgctgtctgt agtggaggtc ctgagcctca
     2341 attatcagta acctttccag ctcgcgtaca actctatatt aaattagcaa aaaatgccaa
     2401 tagagttaat aaaaacctgg tgttacctga tgaatatatc gctctagagt ttaaaggtat
     2461 gagtggcgca ggagctatag aagtagacaa aaatttgacc ttcaggattc gtggattaaa
     2521 taatattcat gtccttgact gctttgttaa tgtcgcactg gaaccagctg atggcgttgt
     2581 tgactttggt aaaataaatt cccgaacaat taaaaatacc agcgttagtg agacgtttag
     2641 tgtagtcatg acaaaagatc ctggtgcggc ctgtactgag caattcaata ttttagggag
     2701 ctttttcact acggatattt tgagtgatta tagccatctg gatatgggta atggactgct
     2761 attgaagata tttcataaag atggaacagc aacggaattt aaccgcttct cacaatttgc
     2821 ttctttttca tcgtctagtg caccttcggt caccgcacca ttcagggcag aacttagtgc
     2881 gaacccggca gagactgttg ttgagggacc gtttagtaaa gacgtaatcc tcaaaatcac
     2941 ctat
//
LOCUS       DAJVRF010000250         2936 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJVRF010000250 DAJVRF010000000
VERSION     DAJVRF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.254"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            35..262
                     /locus_tag="OQN13_003959"
     CDS             35..262
                     /locus_tag="OQN13_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCS1617163.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(263..637)
                     /locus_tag="OQN13_003960"
     CDS             complement(263..637)
                     /locus_tag="OQN13_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HCS1617164.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(720..1766)
                     /gene="mdtG"
                     /locus_tag="OQN13_003961"
                     /pseudo
     CDS             complement(720..1766)
                     /gene="mdtG"
                     /locus_tag="OQN13_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2097..2672)
                     /locus_tag="OQN13_003962"
     CDS             complement(2097..2672)
                     /locus_tag="OQN13_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1617165.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          761 a          695 c          687 g          793 t
ORIGIN      
        1 ttatgccggt atttttttac ggaagaggta tcaaatgcgt ttaattgtgg tgagcatcat
       61 ggtgaccttg ctgagtggct gtggcagcat cattagccgc actataccgg ggcaggggca
      121 cggcaaccaa tattatcccg gtgtgcaatg ggatgtgcgt gactccgctt ggcgttatgt
      181 cacaatcctt gatctgccat tctccctggt ttttgatact ttactgctgc cgatcgacac
      241 tcatcatggc ccgtatgagt gattaacgtt catcccactc atcagctgca gcgtgtcctt
      301 cctcggtatc cagcggtggc tcaagctgaa attccccctc gtcccattca tgtaatgtat
      361 tctcttcctg ccactcctgg cggatctcta tctcatcata gtcgccatca aaaacacttt
      421 gcgcggcttc accgctaagc ataggtaaac attcaccttc ttccccttcg tcggcaaaaa
      481 actcaacttg ccacatgatg tcgccgtcct gcaaaacgta tttttgggca ttgaactgtt
      541 gcacattcgc atcttcggcg tcgatgccgg ggttgtctgc aagaaattct tcgcgtgcag
      601 cgtcaatggc ttcttcaagc gttgcgtaca tggtcatcac gattctccct ttgagttgat
      661 gaggtttcag ggaaaaggat agctgattct ccgcttttgc aagtatgaaa ggcgaaaaat
      721 cagttcgata cctggggtat tcgacgacga cgtagactgt tccatgaata gactgcgttg
      781 aataacacta cgccagcggt gacgagaaat accgctctga aaccgtagtt cgctgaaatc
      841 gctgctccca tcaatggtcc ggtaacgttg ccaatatcac gaaacgattg gttatagctg
      901 aagatacgtc cggcgatttg gttgctcgag ttataaacta acagtgtctg tacggcgggg
      961 agtagtgcac catcggcggc accgagcaaa aaacgtaaaa tcccaagttg caatggcgtc
     1021 tgaacgtaag acattgggat caacagcagt acagaaaaga tcagcgccgt aatcaggatc
     1081 ttttcgggtc cgattcgatc gccaagtttg ccgagtcgtg gtgcacttag cagagccgcc
     1141 acgcctggca ccgaggcgat catgccactg ataaaggcga cgttactgac gttacccgcc
     1201 agttcgcgga catacagcgt cagaatgggg gcaattgagc ccgtcgccac ctggatgatt
     1261 aacgtagtga caaacaggct gagtaccagt ttcgggtttt taagtgatgt caccacttcc
     1321 cgcatgtgca gcatctcttt tttgctgatc ggctggaatt tttctcttat gcaaaacagg
     1381 gtgacgaaaa agcagagtat gagcacactg gcggtaataa agaataccgg acgtaagccg
     1441 tagctatcgg cgagcaggcc gccagccatt gggccgagca acgcaccact aacgccgcct
     1501 gtggagagcg tacccagcgc ccagccgctt ttattacgcg gtacttgtgt ggcgataaga
     1561 gcattagcgt tggggacaaa tccgccaagt aacccaagaa gcgcccgcag gatcaaaaac
     1621 tgccagatat tttgtgccag ccccatcaac accatcacga tgcccatgcc gagggcagag
     1681 cgtaatagca tgagttttcg gcctttacgg tcggcgagtc caccccaaaa cggtgaggcg
     1741 atggccgaaa ataaaaatgt aatgctcata tatgctatct taatgcacta cctgataagt
     1801 gagcctatac aggttattgc tgtatgttta tacagtatta ttggtagaag actgtgtaga
     1861 tttggtatag agatgaatag atactattaa ggtaacttat tgaaatcaat aaagattctt
     1921 gtgcagtctg aatagataac ttagcgttat gtctagtcgt ttccctctcc attcctaaaa
     1981 agtgctgtat actggcctat acaacgctat aaagacctct acatagcgcc ttgatagcta
     2041 tgtcacaggt acaaaaaagc cccctataaa ggaggctgtg tagtctatta agggtcttac
     2101 agggtcttgg tccagtgtct gctaacagtc cttacagaga ggttaagaac cttagctacc
     2161 tgagactgag atagtcccct ttccttgtac tctaagaccg ctctagttgt gtcactcgct
     2221 ataggtcttc caagtacctt accttgaact ctagcacgct ctagaccctg tttagtgcgc
     2281 tctctgatac ggtttctttc aaactctgca aaggtactaa atagctgtag aaccaactta
     2341 ccctctgagc ttgaaaggtc tttaacgggt aaatctaaac agatgagctt cacaccctta
     2401 tcattaagaa gggtcactgt attctgtagg tcgatgttat cccttcccag acggtcaagt
     2461 ttaagaacaa caagggtgtc accttcttct agcttatggt tgaccagatt cgcaaatccg
     2521 tccctatcca tagctgacac tgaaccagag acaacctcct ctataactcg tgacttagga
     2581 atatcatagc cagccttctt aatggcttga atctggttct ctgtggtgga agtctctact
     2641 gatacacggc agtaagcaaa ggttcgtgac atagatatcc ctctgggtag tcattaaacg
     2701 gttagtttgt cttgtctata gacattaaag caaatggtag atttgttgtc tatagctata
     2761 gtgactcttc tatgtagaca ttaaacggac gtttcttgtc gtgggtcaca tagatacttt
     2821 tagggcttct gtcaagtgat attcgtagat tttgcaaggg ggaggtgtaa ggctgaaaca
     2881 gggctactaa gaacacatac atctaaggct ggaaagtacc tgagcagata ttcttc
//
LOCUS       DAJVRF010000251         2868 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJVRF010000251 DAJVRF010000000
VERSION     DAJVRF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.255"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            24..588
                     /locus_tag="OQN13_003963"
                     /pseudo
     CDS             24..588
                     /locus_tag="OQN13_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            592..1167
                     /locus_tag="OQN13_003964"
     CDS             592..1167
                     /locus_tag="OQN13_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCS1617166.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1209..2261)
                     /locus_tag="OQN13_003965"
     CDS             complement(1209..2261)
                     /locus_tag="OQN13_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCS1617167.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2486..>2868
                     /gene="lpxL"
                     /locus_tag="OQN13_003966"
     CDS             2486..>2868
                     /gene="lpxL"
                     /locus_tag="OQN13_003966"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCS1617168.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGIL"
BASE COUNT          717 a          714 c          692 g          745 t
ORIGIN      
        1 gcaaattcgt attggatgca cttatgtcat tcacaaatac ccctgaacgc tatggtgtta
       61 tatctgcggc cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc
      121 tgtggatggt cacgctcagt tattacgatg gctggtatca caaagcaccc gaactgcata
      181 aaagcatcgg tattttgtta atgatggggc tggttattcg cgttctgtgg cgtgtcatat
      241 ctccaccgcc gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaggctg
      301 gacatctcgc attgtaccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa
      361 ctgccgatgg taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg
      421 acgctggcgc acaggcagac tttgccggtg ccctgcattt ttggctcgcg gagcgtcgta
      481 gtactgtccg ttatgcacgg atttatggcc ctgaagcatc atttcatcga taaagacgac
      541 actctgaagc gcatgctggg aaagtcgtca tctgactatg gagtataaaa aatgaaaaaa
      601 agcctgcttg gtttaacctt cgcgtccctg atgttctctg ccggttcagc ggttgccgcc
      661 gattacaaaa ttgacaaaga aggtcagcac gcctttgtta atttccgcat ccagcacctt
      721 ggctatagct ggttatacgg cacctttaaa gatttcgacg gtacttttac ctttgacgaa
      781 aaaaatccgg ctgccgataa agtgaatgtg acaattaaca ccaccagcgt cgatactaat
      841 cacgccgaac gcgataaaca tcttcgcagt gcagatttcc tcaataccgc aaaatatcca
      901 caggcaacat tcacctccac cagcgtgaag aaagacggtg acgaactgga tattaccggc
      961 gatctgacgc tgaatggcgt aaccaaaccc gtcacgctgg aagcgaaatt aattggtcag
     1021 ggcgacgacc catggggtgg taaacgtgca ggcttcgagg ccgaaggcaa aattaagctc
     1081 aaggacttca atatcaagac agatttaggt ccagcttctc aggaagtgga tctgattatt
     1141 tcagtggaag gcgtacagca gaagtaagcg tgaaatgttg ccggatggca cgcatccggc
     1201 aatgatattt attctgttgg atcaggaatg cccagtgttg tattcagacg tccacgagac
     1261 ttattaaaga tcttattgcc attttcacgt cctgcccgac ggcgtcgctg ttcttctggc
     1321 ggtaacgcgc tttcttcgca gcaaatctca ctacaacaac ctttgtattt ttccgcgcat
     1381 actggacact gaataaacag caggtggcag ccatcatttt tacagttggt atggctgtcg
     1441 cacggcgcgc cgcactggtg gcaatgcgcg ataatctcat ccgatatacg ttcgcccatc
     1501 cgctcgtcaa aaacaaaatt tttgccaata aaacgcaccg gcaagccctg ctcgcgcgcc
     1561 ttacgggcgt attcaataat tccgccctcg atatgccaga ctttgttgaa tccgttatgt
     1621 ttcatccagg cactggcctt ttcacaacga atgccgccgg tgcagtacat gacgattttt
     1681 ttatctttat gtgcctgcat catctcgact gcttttggca gctgctcacg gaaggtatct
     1741 gccggaattt cgagcgcgtt ttcaaagtgc cccacttcat actcatagtg gttacgcatg
     1801 tcgataaata gtgcatcggg atcgtcaagc atggcgttca cttccgccgc ttgcagatac
     1861 tcaccaacat tgctggcatc aaagtgagga tcgtcaatac cgtcggcaac aatgcgatcg
     1921 cgtaccttca tgcgcagtac ccagaaggat ttcccgtcat catccaacgc gatattcagg
     1981 cgtaaaccct ctaaagccgg gtcgaaggca tagagctgcg cgcgaaatgt ttcaacattg
     2041 ctcgcaggta cgctgatttg cgcgttaatg ccctcatgcg ccagatacac tcgcccaaaa
     2101 acattcagcg cggtaaacag ctgatataaa gcgtcacggg tcgccttagg atcggcgatg
     2161 tggaaatact tgtaaaacga aatggtggtt cgcggttcgc tctcaaccaa cattttggct
     2221 tttaacgcgt cgttggaaat gcggttgtgt aacactggca tggtgtacgg ttcctgcgag
     2281 atgggaaagt aaaaatccgc ggcatgatat agcaattatc gataattaac atccacacat
     2341 tttacgctac atttgcgcat taaaaattat ttgttattta caagcgcggc aatttcgccc
     2401 agtcttcagc cacaaatttt ggttgcgggc gaaaaaatgc gacaatacat acaattgccc
     2461 gaataggttg aaaaacagga ttgatatgac gaatctaccc aagttctcca ccgcactgct
     2521 tcatccgcgt tattggttaa cctggttggg tattggcgta ctttggttag tcgtgcaatt
     2581 gccctacccg gttatctacc gcctcggttg tggattagga aaactggcgt tacgttttat
     2641 gaaacgacgc gcaaaaattg tgcatcgcaa cctggaactg tgcttcccgg aaatgagcga
     2701 acaagaacgc cgtaaaatgg tggtgaagaa tttcgaatcc gttggcatgg gactgatgga
     2761 aaccggtatg gcgtggttct ggccggaccg ccgaatcgcc cgctggacgg aagtgatcgg
     2821 catggaacac attcgtgacg tgcaggcgca aaaacgcggc atcctgtt
//
LOCUS       DAJVRF010000252         2798 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJVRF010000252 DAJVRF010000000
VERSION     DAJVRF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.256"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            1..618
                     /locus_tag="OQN13_003967"
                     /pseudo
     CDS             1..618
                     /locus_tag="OQN13_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3289 family protein"
     gene            615..1094
                     /locus_tag="OQN13_003968"
     CDS             615..1094
                     /locus_tag="OQN13_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HCS1617169.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(1148..2098)
                     /gene="corA"
                     /locus_tag="OQN13_003969"
     CDS             complement(1148..2098)
                     /gene="corA"
                     /locus_tag="OQN13_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCS1617170.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            2468..>2798
                     /locus_tag="OQN13_003970"
     CDS             2468..>2798
                     /locus_tag="OQN13_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617171.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          843 a          620 c          590 g          745 t
ORIGIN      
        1 ttccggaaca aaaatatcac aacgtgaatg cactgatatt ttatttgcag aaatgaaaga
       61 actagcaaaa atgttttcct tttttggaca atataaaaca cttatcgaag atctgattga
      121 acattttaga tatggaaatg gcagcaattt tcatagccaa caattaaatt tatcttttca
      181 tgaaaaaata aatatggtta taatagtcca atacgaataa tcaaagaatg tatcgaaaat
      241 ggcattaatt caactccctc gacaggatac caacctctta tcctgcaaag cattaaaacc
      301 aaattacttt catcaagact aaataaattt aatgacttcg aggacagttt taatggctta
      361 ggaatcagtg ttcatgatat ttcagctcaa aaaatatctc ttttaagttt tcaaaaatac
      421 gctataggat ggagtgcaac aattcatttt gtagcacagg accacttcgg tcttgatgta
      481 actgatatca aaaacaagac ttacagcaaa tatagatttt ttcgcatatg gttcttttta
      541 caacggcata aagactttgc tttcaaacct tttttcacca attttaacac tatcgaaaga
      601 atcgaaaatt atctatgaaa agcaaactcc agaaaattat attatgttta tttcttctat
      661 gctgcatcta taatttatgg acattgcgtc ctgtacagat tttgtataca tactctgatg
      721 ctggcaattc agtctttctg gtcgttgatc atctgccatg gactgacagc gataaaataa
      781 attggtatct gaagcatcag aatgaaatta aaaatcaaca tcccctacca gaaggtagtt
      841 ggcatacctg gtatgtcatt gatatcggta atggcttcac tgactataaa aaatatatcg
      901 aagggccgta tgaagatctc tactgtttcc cgacaattaa gagcaatgat aattgcatcg
      961 tgaaaaatta cttaatggtt attaatgaat acccttatcg taacactcat attggtatta
     1021 acgattttac tgagtatcaa ttaacacaag aaaataaaat agagcgagtt ttcaatccgc
     1081 atgactttaa ataagacaat ttttagaaat aagccggata aaaaaatagc tttatccggc
     1141 actcatatta cagccagttc ttgcgcttaa agtacagata cggtgccagg cccgcgagga
     1201 tcataaagat aatcgcgcct gggtagccga aactccactt cagttctggc ataaactcaa
     1261 agttcatccc atagctggaa gcaacgagcg tcggcggcag gaataccacg gataccaccg
     1321 agaagatttt gatgatgcgg ttctgctcga tgttgataaa acccatcgcc gcctgcatca
     1381 ggaagttcac cttctggaac agggattcgt tatgcggcag cagggattcg atatctcgca
     1441 ggatttcacg cgcctgctcc agttgcccac ccggtaaacg cgctttacgc accaggaagt
     1501 tgagcgcgcg ctgggtatcc atcagacaca ggcgaacttt ccagccgata tcttccagtt
     1561 ccgccagagt ggagagcgcc tcgtcgtact catcgccctg atgcccttcc ataatcaccc
     1621 ggctcaactg ctccaggtcg ctatagatat tttcaatttc atctgccaac tgttcgattt
     1681 tggtttcgaa cagatccagc agcagctcgt aggcgttacc gtctaccatc gactggctac
     1741 gggcacgcat acgatacaga cgaaaagcgg gcagttcacg ctcacgcaga gtaaacagac
     1801 gaccatcacg gatggtaaat gccacagtgg agttaccggc gtgatcttcc gcatcttcaa
     1861 agaagaagaa ggagtgaata tgcaggccgt cgtcgtcttc aaagaaacgt gccgatgctt
     1921 cgatgtcttc cagttccggg cgggttgcca ggctctggcc aagttcagat tgtacgcgca
     1981 gtcgctcgtc gtcgtccggt tcgacaagat caatccatac tgcatttaca aggggttgtg
     2041 actcttcgac ttccagccgg gtcagtcggt tattttccag ttgaaatgcg ctcagcatga
     2101 ccgggactcc caatgcttaa aatatcggac agttcggtgg gcacacagaa acaaattggg
     2161 tttcagacca ttaaacagcc tgactcagcg cgacgggaaa aattgaggtc gctgacaacc
     2221 gctaaggcta tcagcaaaaa gggatagcct taggagttga tcctggatga caggatagtg
     2281 agccagtatc tactgggtgt gtccaaggcg aatgtcctct tagagtgatc gtgcgcgcat
     2341 gttacgccag caaaattttg ccgtcaacac gcaacagaac accacagaac aatagttgaa
     2401 cttcgcgctt tttggtactc gtcaaggtcg ctaacaggat aaaaaaatcc aacatccagg
     2461 acctataatg gcgcatcggc tactcattgg taaaggaatg atcaccttga atctcaaacg
     2521 gatttttctc gccctcacct tacttccctt atttgctgtt gccgctgatg attgcgcact
     2581 ctcagatccg atgctgaccg tacaggcgta taccgttaat cctcaaacag agcgggtgaa
     2641 aatgtactgg caaaaagcca atggcgaagc gtggggaact ttacatgctc tgctggtgga
     2701 tatgaatagt cagggtcagg tgcagatggc gatgaacggc ggcatctatg atgaaagcta
     2761 tgcgccgctc ggtttgtaca tcgaaaacgg tcagcaga
//
LOCUS       DAJVRF010000253         2782 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJVRF010000253 DAJVRF010000000
VERSION     DAJVRF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.257"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..1548
                     /gene="fimD"
                     /locus_tag="OQN13_003971"
     CDS             <1..1548
                     /gene="fimD"
                     /locus_tag="OQN13_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
                     /protein_id="HCS1617172.1"
                     /translation="ILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGLPQEFTA
                     YGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFLYAKSMY
                     TFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYHNLRFSR
                     KDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSLSFSWNE
                     SVGIPDNEHIVGLNVSVPFNVLTKRRYTRENALDRAYASLNANRNSNGQNSWLAGVGG
                     TLLEGHNLSYHVSQGDTSNNGYTGSATANWQATYGTLGVGYNYDRDQHDVNWQLSGGV
                     VGHENGITLSQPLGDTNVLIKALGAGGVRIENQTGILTDWRGYAVMPYATVYRYNRIA
                     LDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPFGSLVRE
                     NSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQAVTVISA
                     VCTHPRS"
     gene            1561..2568
                     /gene="fimH"
                     /locus_tag="OQN13_003972"
     CDS             1561..2568
                     /gene="fimH"
                     /locus_tag="OQN13_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HCS1617173.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPLGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            2579..>2782
                     /locus_tag="OQN13_003973"
     CDS             2579..>2782
                     /locus_tag="OQN13_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617174.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTT"
BASE COUNT          694 a          613 c          720 g          755 t
ORIGIN      
        1 attttacaac gcgaagggcg tttcaaattt gacctgacgg cgggcgattt tcgtagcggt
       61 aatagtcagc aatcatcacc tttctttttt cagggcacgg cactcggtgg tttaccacag
      121 gaatttactg cctacggcgg gacgcaatta tctgccaatt acaccgcctt tttattaggg
      181 ctggggcgca acctcgggaa ctggggcgca gtgtcgctgg atgtgaccca tgcgcgcagc
      241 cagttagccg acgacagtcg tcatgagggg gattccattc gcttcctcta tgcgaaatcg
      301 atgtacacct tcggcaccaa ttttcagtta atgggttacc gctattcgac acaaggtttt
      361 tatacccttg atgatgttgc gtatcgtcga atggaggggt acgaatatga ttacgattac
      421 gacggtgagc atcgcgatga accgataatc gtgaattacc acaatttacg ctttagccgc
      481 aaagaccgtt tgcagttaaa tatttcacaa tcacttaatg actttggctc gctttatatc
      541 tctggtaccc atcaaaaata ctggaatact tcggattcag atacctggta tcaggtgggg
      601 tataccagca gctgggttgg catcagttat tcactctcat tttcgtggaa tgaatctgta
      661 gggatccccg ataacgaaca tattgtcgga cttaatgttt cagtgccttt caatgttttg
      721 accaaacgtc gctacacccg ggaaaatgcg ctcgaccgcg cttatgcctc cttgaacgct
      781 aaccgtaaca gcaacgggca aaatagctgg ctggcaggcg ttggtgggac cttactggaa
      841 ggccacaacc tgagttatca cgtaagccag ggcgatacct cgaataatgg gtacacgggc
      901 agcgccacgg caaactggca ggccacttac ggtacgctgg gggtcgggta taactacgac
      961 cgcgatcaac atgacgttaa ctggcagctg tctggcggtg tggtcgggca tgaaaatggc
     1021 ataacactga gccagccttt aggggatacc aatgttttga ttaaagcgct tggcgcaggc
     1081 ggtgtacgca ttgaaaatca aactggcatt ttaaccgact ggcgcggcta tgcggtgatg
     1141 ccgtatgcca cggtttatcg gtataaccgt atcgcgcttg ataccaatac gatggggaat
     1201 tccatcgatg ttgaaaaaaa tattagcagc gttgtgccga cgcaaggcgc gttggttcgt
     1261 gccaattttg atacccgcat aggcgtgcgg gcgctcatta ccgttaccca gggcggaaaa
     1321 ccggtgccgt ttggatcact ggtacgggaa aacagtaccg gaataaccag tatggtgggt
     1381 gatgacgggc aagtttattt aagtggtgcg ccattgtctg gtgaattact ggttcagtgg
     1441 ggagacggcg cgaactcacg ctgcattgcg cactatgtat tgccaaagca aagcttacag
     1501 caagccgtca ctgttatttc ggcagtttgc acacatcctc gctcataaag gaaattatca
     1561 atgaagataa tctgcagatt attattggca atggcatgtt tgtgtctggc aaacatatcc
     1621 tgggctactg tttgtgcaaa tagtactggc gtagcagaag atgaacacta tgatctctca
     1681 aatgtcttta atagcaccaa taaccagcca gggcagattg ttgttttacc ggaaaaatcc
     1741 ggctgggtag gtgtctcagc aatttgtcca ctcggcacgc tggtgaatta tacataccgt
     1801 agttatgtca ctaactttat tgttcaggaa actatcgata attataaata tatgcaatta
     1861 aatgattatc tattaggtgc gatgagtctg gttgatagtg tgatggatat tcagttcccc
     1921 ccgcaaaatt atattcggat gggaacagat cctaacgttt cgcaaaacct tccgttcggg
     1981 gtgatggatt ctcgtttgat atttcgttta aaggttattc gtccctttat taacatggtg
     2041 gagatcccca gacaggtgat gtttaccgtg tatgtgacat caacgcctta cgatccgttg
     2101 gttacacctg tttataccat tagttttggt ggccgggttg aagtgccgca aaactgcgaa
     2161 ttaaatgccg ggcagattgt tgaatttgat tttggtgata tcggcgcatc gttatttagt
     2221 gcggcagggc cggggaatcg accagctggc gtcatgccgc aaaccaagag cattgcgatc
     2281 aaatgtacga atgttgctgc gcaggcttat ttaacaatgc gtctggaagc cagtgccgtt
     2341 tctggtcagg cgatggtgtc ggacaatcag gatttaggtt ttattgtcgc cgatcagaac
     2401 gatacgccga tcacgcctaa cgatctcaat agcgttattc ctttccgtct ggatgcagct
     2461 gcggcagcca atgtcacact tcgcgcctgg cctatcagta ttaccggtca aaaaccgacc
     2521 gaagggccgt ttagcgcgct ggggtattta cgcgtcgatt atcaatgagg tacggagaat
     2581 gagaagagta ctctttagct gtttctgcgg gttactgtgg agttccagtg gatgggcagc
     2641 tgaccctttg ggaacgatta atatcaattt gcacggtaac gttgttgatt tctcctgtac
     2701 cgtaaacact gcggatattg ataagacggt agatttaggc agatggccta cgacacaatt
     2761 actgaacgct ggcgatacca cg
//
LOCUS       DAJVRF010000254         2753 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJVRF010000254 DAJVRF010000000
VERSION     DAJVRF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.258"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..1415)
                     /gene="otsA"
                     /locus_tag="OQN13_003974"
     CDS             complement(<1..1415)
                     /gene="otsA"
                     /locus_tag="OQN13_003974"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HCS1617175.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFP"
     gene            complement(1390..2190)
                     /gene="otsB"
                     /locus_tag="OQN13_003975"
     CDS             complement(1390..2190)
                     /gene="otsB"
                     /locus_tag="OQN13_003975"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCS1617176.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(2357..>2753)
                     /gene="araH"
                     /locus_tag="OQN13_003976"
     CDS             complement(2357..>2753)
                     /gene="araH"
                     /locus_tag="OQN13_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCS1617177.1"
                     /translation="RNTLAIGGNEEAARLAGVPVVRTKIIIFVLSGLVSAIAGIILAS
                     RMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKISYVVAGILILGTVENAMNLLNIS
                     PFAQYVVRGLILLAAVIFDRYKQKAKRIV"
BASE COUNT          682 a          714 c          654 g          703 t
ORIGIN      
        1 tttggaaagg tagcaacttt atcgcgctgc tggctttccg cgcttcgcgg aactatctgc
       61 tttaggtcgc taatgaagca ctcctgccag tggttaatat cgtttttcac gataacgtcc
      121 agcatttctg catgacggga aatacgttcc gccagcgaca tagtcaatgc acgatccagc
      181 gcagctgcaa cttcgtcacg atcgtagggg ttaacaatta acgccgacgt taactcgttt
      241 gccgctcccg caaattgcga aagaacaaga acgcccgggt tggctgggtc ctgagcagca
      301 acatactctt ttgctaccag gttcatcccg tcacgcagtg gcgtcactaa gcccacgtca
      361 gagtagcgga atattttcat cagtaattta cggtcaaaat gctgattcaa ataataaagc
      421 ggcgtccagc ctaattgccc gtatttacca ttaattcgtc cagcttcatt ttcgagctga
      481 tgacgaatat cctgataggc ttgcacatca ccacgcgacg ttggtgcaat ctgggtataa
      541 cgaattttac catgatgctg cggatatttt tccagcaacg cttcataggc gagaaaacgc
      601 tctggcaaac ctttggaata atccagccgt tcgacagaaa agatattttg tacgtttttc
      661 agttccgctt taagttgcgc cagttttggc ggcagtggcc cggcagcctg tttggctatt
      721 tctttcggtt caatgccgat cgggtagact tctgttcgaa atgctttgcc ccaggctgta
      781 tggctttttg cgctacgtgt cgtgacgcgg gtcaggttag aaagacaatc caggaacgcc
      841 agacgatcgt tttctgtctg gaaacccagc aaatcataat cacaaagctg ttcaagcaag
      901 gtgtcatatg tcggcagcgc gttgaagatt tccggtgtcg ggaaaggaat atgcagaaag
      961 aaaccaatgc gattattcac tccccgtttg cgtaattcat gcgcaaatgg caacaggtga
     1021 taatcgtgga tccagataat gtcatcgtct tgcaacagcg gcagtaattt atctgccagc
     1081 aacgcattta cgcgtagata gccgtcccag gcaggacgct gaaattgcac cagatcgagc
     1141 cgataatgaa aagcgggcca gagaacggca ttggagaatt ggttgtagta ttcgtcaagg
     1201 tcctgttcgc tgaggttaaa agaggcccac gtaatgttac cttttttcac cttttttagc
     1261 ggctgatcct cattccctgt ttcaccactc cagccaaacc acagtccgcc tgcggctttc
     1321 agtgccccca gtatgccaac ggcaaggcca ccggcactgg cggcgtgctc gtctggtggt
     1381 gcaatccggt tagatactac gactaaacga ctcatagtca tcactcctgt tattttctct
     1441 tttttgttgt aatgcggtgg ttatcatttc aagccagctc cagacatccg gcacacccgc
     1501 cagtcgccat gatgcctgag ttgcacctgt gccaattttt actgacattc cgcccagtcg
     1561 gttaacgact gcgaagccag attcatcggt taaatcatcg cccagaaata cgggcgttcg
     1621 cccgataaag ggagcttcct gcataaaagc tgcaattgcc tcacctttac tggtacctct
     1681 cggtttgatc tcgacaacac actttccctg ctgtaacgcc atttgtggcc agatctgagt
     1741 aatacgttgc gctaatgtca ttaatgcgtc ttcatgctgc ggagcctgac gataatgcag
     1801 cgcaaaagcc atccctttcg cctccagctc cgcgccggga tactgagcga tgactgtatg
     1861 cagttgcacg ctaatatcac gcgcaatcgc atccggcaga tgaacgatat gtgttttacc
     1921 attgatgtca cggcgctccg ccccatgcac gcccgctaac gggaagcgat aaggttttgc
     1981 cagtgcgtca agctccacca ttgagcgccc tgatatcaat gccaatgcac catcacttgc
     2041 ggttgccagt agctgtagtc cttgcagaat attgtcaggc acgacgacct gatcgggatg
     2101 cggtttgatt tccgccagcg ttccatcaag atcaaaaaac caggcatatt tcgcggatag
     2161 ttcaggggtt tcggttaacg gttctgtcac ccggctctcc tccttctctt ttcttattgt
     2221 tttgcgcaga acgcgcttta cagacatgta agtatagaca gtgtgacggg ggtcgccatt
     2281 tgcaatgaag aacagccaac cgttgtggcg gctggctgtg gtgggaaaaa acgttaaatt
     2341 gttgtggaaa aaagcatcag acaatgcgtt tcgctttttg cttgtaacgg tcgaagatca
     2401 ccgctgccag caggattaag ccgcgaacca cgtactgcgc gaaaggagaa atattaagca
     2461 ggttcatggc gttttccacg gtgcctaaaa ttaagatacc cgccaccaca tatgagattt
     2521 ttccgatgcc acctttcaga gaaacgccac ctaaaacgca ggcggagata acaataagct
     2581 cataaccaat cgacgtcatt ggctgcccac tagtcatacg tgaagccaga ataattccgg
     2641 ctatcgcaga taccaggcca gagagaacaa agataataat tttggtgcga acaaccggta
     2701 cacccgccag acgcgcagcc tcttcgttcc cgccaatcgc cagggtgtta cga
//
LOCUS       DAJVRF010000255         2748 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJVRF010000255 DAJVRF010000000
VERSION     DAJVRF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.259"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..1036
                     /locus_tag="OQN13_003977"
     CDS             <1..1036
                     /locus_tag="OQN13_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCS1617178.1"
                     /translation="DLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKGKSAARIKEE
                     DFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLPLEQDERITA
                     ILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDELIGVDLTSGE
                     DEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRGDGAILTATQ
                     NGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMITDAGTLVRT
                     RVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAAEGDDEIAPE
                     VDVDDEPEEE"
     gene            1244..>2748
                     /locus_tag="OQN13_003978"
     CDS             1244..>2748
                     /locus_tag="OQN13_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HCS1617179.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINSQGVAKLLRNETLTSLPKNLEPVFYNAA"
BASE COUNT          681 a          668 c          758 g          641 t
ORIGIN      
        1 agatctgatc acccaggaag atgtggtcgt gacgctctct caccagggct atgttaaata
       61 tcagccgctt tctgaatacg aagcgcagcg tcgtggcggg aaaggtaaat ctgccgcacg
      121 tattaaagaa gaagacttta tcgaccgact gctggtggca aacacgcacg accatattct
      181 gtgcttctcc agccgtggtc gcgtctattc gatgaaagtt taccagttgc cggaggccac
      241 tcgtggcgcg cgcggtcgtc cgatcgtcaa cctgctgccg ctggagcagg acgaacgtat
      301 caccgcgatc ctgccagtga ccgagtttga agaaggcgtg aaagtcttca tggcgaccgc
      361 taacggtact gtgaagaaaa ccgtcctcac cgaattcaac cgtctgcgta ccgccggtaa
      421 agtggcgatc aaactggttg aaggcgatga gctgatcggc gttgacctga ccagcggcga
      481 agacgaagta atgctgttct ccgccgaagg taaagtggtg cgctttaaag agtcttctgt
      541 ccgtgcgatg ggctgcaaca ccaccggtgt tcgcggtatt cgcttaggtg aaggcgataa
      601 agtcgtctct ctgatcgtgc ctcgtggcga tggcgcaatc ctcaccgcaa cgcaaaacgg
      661 ttacggtaaa cgtaccgcag tggcggaata cccaaccaag tcgcgtgcga cgaaaggggt
      721 tatctccatc aaggttaccg aacgtaacgg tttagtggtt ggcgcggtac aggtagatga
      781 ctgcgaccag atcatgatga tcaccgatgc cggtacgctg gtacgtactc gcgtttcaga
      841 aatcagcatc gtgggccgta acacccaggg cgtgatcctc atccgtactg cggaagatga
      901 aaacgtagtg ggtctgcaac gtgttgctga accggttgac gaggaagatc tcgataccat
      961 cgacggcagt gccgcggaag gggacgatga aatcgctccg gaagtggacg ttgacgacga
     1021 gccagaagaa gaataatttt acttcttcat gccaaaaggg agctatctcc cttgtttgaa
     1081 ttgaaaagtc caggctgcaa agtctgggct tttgtcgtat tagggcacgg taaagtttgg
     1141 ctgtgcccgt aaaaaatggc tggctataca caaggaatgt ggcaatgagt ggtgaaaaaa
     1201 agcgaaaggc tggcggttct atggtcttgt aggttttggc gcaatagtac tgctttccgc
     1261 tggtgtctgg gcgttgcaat atgctggcag tgggccagaa aaaacgttgt cgccgctggt
     1321 ggtgcacaac aatctgcaaa tcgatctcaa tgagccggac ctctttctcg acagcgactc
     1381 tctgagccag cttcccaaag atctcctcac cattccgttt ctccacgatg ttctgagtga
     1441 agatttcgtt ttctattatc agaatcatgc cgatcgtctg ggcattgaag gcagcattcg
     1501 tcgcattgtc tatgaacacg atctcacgct gaaagataaa ctcttttcgt cactcttaga
     1561 tcagcccgcg caggctgcgc tatggcacga taaacaaggc catctttcac attacatggt
     1621 gctgatccag cgcagtggtt taagcaaact gctggagcca ttgttgtttg ccgctaccag
     1681 cgacagccag ttaagcaaaa cggaaatcag tagcatcaag ataaatagtg aaactattcc
     1741 tgtttatcag ttgcgctata acggcaataa cgccctgatg ttcgcgactt atcaggacaa
     1801 gatgctggtg ttttccagca cggatatgtt gtttaaagat gatcagcagg ataccgaagc
     1861 cacggcgatc gcaggtgatt tgttgagcgg caaaaaacgc tggcaagcaa gctttggcct
     1921 ggaagagcgt gctgctgaaa aaacgccagt acgccagcgc atcgtggtca gcgccaggtt
     1981 gctgggtttt ggctaccagc ggttaatgcc ttcttttgct ggcgtacgct tcgaaatggg
     2041 taacgacggc tggcacagtt ttgtggcgtt aaatgatgaa tccgccagcg tagataccag
     2101 tttcgatttt acgccggtct ggaacagtat gcctgccggg gccagcttct gtgtggtggt
     2161 gccgtattca cacggtattg ccgaagagat gctttcgcac atcagccagg aaaacgacaa
     2221 gctgaatggg gcgttagacg gtgccgcggg gctgtgctgg tatgaagact caaaattgca
     2281 aaccccgctg tttgtcggtc agtttgatgg cactgccgaa caggcgcaat tgccagggaa
     2341 actgtttacg caaaatattg gtgcgcacga aagcaaagcg ccagaaggtg ttttgccgat
     2401 aagccagact cagcagggcg aagcgcaaat ctggcgtcgc gaagtgagtt cccgatacgg
     2461 tcagtatccg aaagcgcagg cggcgcaacc cgatcaatta atgtcggatt attttttccg
     2521 cgtgtcgctg gcgatgcaaa acaaaacgct gcttttctcc ctcgatgaca cgctggttaa
     2581 taacgccctg caaacactga ataaaaaccg cccggcaatg gtggatgtaa tacccactga
     2641 tggcatcgtt ccgctctata tcaattcaca aggcgtggcg aaactgctgc gtaacgaaac
     2701 gttgaccagt ctgccgaaga atctcgaacc ggttttttat aacgccgc
//
LOCUS       DAJVRF010000256         2739 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJVRF010000256 DAJVRF010000000
VERSION     DAJVRF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.260"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..1007)
                     /gene="shiA"
                     /locus_tag="OQN13_003979"
     CDS             complement(<1..1007)
                     /gene="shiA"
                     /locus_tag="OQN13_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HCS1617180.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
     gene            1322..2374
                     /locus_tag="OQN13_003980"
     CDS             1322..2374
                     /locus_tag="OQN13_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HCS1617181.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
BASE COUNT          849 a          592 c          616 g          682 t
ORIGIN      
        1 gccataaaga aaggaaatgc gctcaacgtt ccgattaacg cacctgtgat ataaacccta
       61 cggcgaccaa aacgatcggc aagccaggca aaacagggaa ttgtcaggca gcttaatcca
      121 cctaccagca aaccaatatt aaggaaaagt tcgcgcggta gccccatatt ctgggttgaa
      181 taattaagtg caaaggcagt aacgatgtac atcgtcagca attcgcacag tcgtagcgca
      241 ataatcttca ggaaagcacc gggatgtcgt aacagcgctt cgataaccgg gatgcgtttt
      301 ttcgcggcag cttgataatg ttgctgttgt tcaaattccg cggactcctc catgccattg
      361 cgcacccaca atgctcccag taccagtacg atgctaaaca ggaaaggaat gcgccagccc
      421 cagcttaaaa actgttcgtc agtcgtcatc atactgatca atgaaaccag tccggttgaa
      481 agcagtaaac ctacaccgta gccaacttgt acaccgctac tgtaaaaggc ttttttattt
      541 ttcggtgcac tttcaacgga aagcaacgcc gcgcctcccc attcgccgcc gacggcaaat
      601 ccctgaatga cacgcagtgt caccagtaaa ataggtgccc accacccaat ggtcgagaat
      661 gaaggaagaa taccaatcaa ggctgtcgcg atgcccatca tccagacggt cagcattaac
      721 atgcgcttac gtcccagtcg gtcgccaaag tgaccgaaaa tgacaccgcc gagcggacgg
      781 aaaaggaatc cgacgccaaa ggtagcaaat gcggcgagcg ttcccatcgc cgggcttact
      841 tgcgggaaaa actcgcgatt aaacaccagt gcggcggtga tgccatagag taaaaaatca
      901 taccagtcga cgacggcacc agcgaagctg cctaacgcag ctcgtcgggc gcgacttaac
      961 gaaagcgtcc cttcatcggg acgagtggag atgagcgtgg agtccataat gatcctgttt
     1021 gtactgtctt tttataatta gtatggaaaa ggtaactcac attgccgtcg atctataaac
     1081 gaaccttatg agactacctc gacagagcgc atctgcaaat gattttatgg ctgattagcg
     1141 gcaacatgaa aaattcgatg gaagatgaaa gcgcaggagt tgctggtgtt gtttaaggat
     1201 taagcagagg acccacaggt tagagatatg aaacataaaa gtccgcatat gcacaaaatg
     1261 gcaccctgat gagtctgata gactgcattg cattataacc agtagggagg cgaggttagg
     1321 tatgttttta gcatcattgt tgagacgtat tgcatttagt tactacgatt ataaagctta
     1381 taatttcaat attgaaaaaa cagactttgt tgtcatccat attcccgatc agattggcga
     1441 tgctatggcc atctttcctg ttattcgggc gcttgaattg cataaaatta agcatctttt
     1501 aattgtaacg tcgacaatta atttagaagt ctttaatgcg cttaaacttg aacagactaa
     1561 attaacatta gtcacaatga ctatgcagga tcacgcaaca ttaaaagaaa taaaagattt
     1621 agcaaagaac ataacacagc aatacggtac gccggatctt tgtattgagg ggatgcgtaa
     1681 aaagaacctg aaaacgatgt tatttatcag tcagttgaaa gcaaaaacga attttcaggt
     1741 tgttggtata accatgaatt gctactcccc tttgtgcaag aacgcgtccc gtatggatca
     1801 gaaactccgg gctcccgtac ctatgacatg ggcatttatg atgcgtgagg cgggttttcc
     1861 agcagtcagg ccaatatatg aattgccact aagtgaggat gtactcgatg aggtgcgcga
     1921 ggaaatgcga tcgttaggat cttacattgc gttcaattta gaaggtagct cgcaggaacg
     1981 tacattttca ttatcgattg cagaaaatct aatagcaaaa attcaaagtg aaacagatat
     2041 accaatagtg atcgttcatg gacccaaagg tgaagataaa gccagggcat tagtggattg
     2101 ttataataat gtctaccgtt tatccttacc accctcgatt aaacgttcag cagcaatcat
     2161 aaaagatgct tatatcgcaa taactcctga cacctcaata ttacatatgg caagtgccta
     2221 taatacccct gttgttgcaa tttatgctga ttacaaaacg cgatggcccg caatggccga
     2281 tgtctcggag tcagtcgtcg ttgggcaaaa aattgacaat ataagtctgg atgaattcgc
     2341 aaaggcatta aaaagtgttt tggcgagaat atgagacagt acttatgcat agtaatgccg
     2401 atctgttatg aacggtggtg tcatcaatac tctgaggctg gtcaatcatc acagaaaacc
     2461 agcctttgaa tcaaagcagt actcaccgga tacgatcata gtcacatcga acgcaattct
     2521 ggcggtgccg catttttatt tattcatttt ctgtgagaga agggcaagct ctatagaggg
     2581 aatgtaaaaa agagagctcc gaatggagca ctctttaaga aattataacg gaaaatcaga
     2641 ggtttttata acatgtcgca taagcaaacc caagcttttc aaatacgctt tgatcacctg
     2701 ttgccaggga aactacgtat tgttcgcttg agtttgcgg
//
LOCUS       DAJVRF010000257         2690 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJVRF010000257 DAJVRF010000000
VERSION     DAJVRF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.261"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            230..769
                     /locus_tag="OQN13_003981"
     CDS             230..769
                     /locus_tag="OQN13_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCS1617182.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="OQN13_003982"
     CDS             complement(995..1318)
                     /locus_tag="OQN13_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCS1617183.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="OQN13_003983"
     CDS             1604..1798
                     /locus_tag="OQN13_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCS1617184.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcaggga caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttggtagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAJVRF010000258         2663 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJVRF010000258 DAJVRF010000000
VERSION     DAJVRF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.262"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(261..452)
                     /locus_tag="OQN13_003984"
     CDS             complement(261..452)
                     /locus_tag="OQN13_003984"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617185.1"
                     /translation="MGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAY
                     QPRRETFRRLYQQLLPLMA"
     gene            complement(385..1716)
                     /gene="xylB"
                     /locus_tag="OQN13_003985"
     CDS             complement(385..1716)
                     /gene="xylB"
                     /locus_tag="OQN13_003985"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCS1617186.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQKLDYRTGGGCGASTGRSKAGADCGESREIAH"
     gene            complement(1788..>2663)
                     /locus_tag="OQN13_003986"
     CDS             complement(1788..>2663)
                     /locus_tag="OQN13_003986"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCS1617187.1"
                     /translation="YGAGAATNPDPEVFSWAATQVVTAMEATHKLGGENYVLWGGREG
                     YETLLNTDLRQEREQLGRFMQMVVEHKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVY
                     GFLKQFGLEKEIKLNIEANHATLAGHSFHHEIATAIALGLFGSVDANRGDAQLGWDTD
                     QFPNSVEENALVMYEILKAGGFTTGGLNFDAKVRRQSTDKYDLFYGHIGAMDTMALAL
                     KIAARMIEDGELDKRIAQRYSGWNSELGQQILKGQMSLADLAKYAQEHNLSPVHQSGR
                     QEQLENLVNHYLFDK"
BASE COUNT          659 a          765 c          597 g          642 t
ORIGIN      
        1 cttagcaatg ggcatgagat ccatagccca accagataaa ccagcgcccc gacaacaaat
       61 aatagttttg ccacagtttt tgaggttttc ataacgatct ccatatctac cagcgataca
      121 ttacgagtaa ccaacgaaag acaaaactga agaatgccat taacaaatga ttttcagaat
      181 aaattcatac taaatattaa ttaattagtg agatatatag atgtgaatta tcccccaccc
      241 ggtcaggcag gggataacgt ttacgccatt aatggcagaa gttgctgata gaggcgacgg
      301 aacgtttcac gtcgtggctg ataagcggca taacgctgcg catctggtag atgcgactgt
      361 tctaacggta gctgcggcaa caactcaatg agcgatttct ctggattcgc cgcaatctgc
      421 gccagccttg ctgcgcccag tgctggcccc acatcccccc cccgtacggt aatcgagctt
      481 ctgaccgctg atatccgcca gcatctgacg ccagtactca ctacgcgccc cgccaccaat
      541 caacgtaaca ctttgcggtt taatgccgca ggcatgcacg acatccatgc catctgccag
      601 cgcataaccc acgccttcca gcactgctcg cgccagttca ttggggccat gttgatgagt
      661 caaaccaaag aaaaccccct tcgcctgggg attattgtgt ggtgtacgct cgccggaaag
      721 ataaggcaga aaccaaactg gctcgacact ttcatcagcc tgttgagctg cagcgattaa
      781 agctgggaca ttgcacaggc cggttaattt cgcggcccaa tccagacacg atgctgcact
      841 cagcattaca gacattaaat gccaacgctg cggtagcgca tggcaaaagc tgtgtacagc
      901 gctttctggc ttgcttaaga acccttcgct gacagcaaaa tagacccccg acgtccccag
      961 cgataacatt gcctgattag catcaaccat tcccacacca actgcaccag ctgcattgtc
     1021 gccaccgcct gcgacaactg gcaccgtcgc cataccccac gctttcgcaa cttcaggtaa
     1081 caaagcacca gtaatttcgc tgccttcgta taatgcgggc atctggtcac gagataagtc
     1141 gcaagcctgc agcatgacgt cactccagtc acgctttgcg acatccagcc acatggtgcc
     1201 agctgcgtca gacatatcgc tggcaaactc ccccgtcata cgcagacgca agtaatcttt
     1261 cggtaataat actttgtcga tttgacggaa tatctccggc tcatgccgct gaacccatag
     1321 caatttaggc gcagtaaatc cgggcatcat caggttgccg gtaatcactc gtgattgcgg
     1381 aactctcgct tccagcaaag tgcactcttg cgcacagcgc ccgtcgttcc acaaaatggc
     1441 agggcgcaat acccgttgtt gagcatccag taaggttgct ccatgcatct ggccggcaat
     1501 acccaatgct ttaacgtcct gcagagaatg ctgatcgccc agagctttca ttgcgcgatc
     1561 agttgcctgc caccactgtt ccgggtcttg ttccgaccag agtggatgcg ggcgcgaaac
     1621 ggtcagcttt tccgtttgcg aatcaaccac ctcaccctgc tcgttgagca aaataacttt
     1681 tacgcccgag gtgccaagat ctatcccgat atacatatcg atcgttcctt aaaaaaatgc
     1741 ccggtatcgc taccgataac cgggccaacg gactgcacag ttagccgtta tttgtcgaac
     1801 agataatgat ttaccagatt ttccagttgc tcctggcgac cactctgatg caccggagac
     1861 aaattatgtt cctgagcata tttggctaaa tctgccagtg acatttggcc tttcaggatt
     1921 tgctggccca attcgctatt ccagccggaa taacgctgcg cgatgcgttt atccagctcg
     1981 ccatcttcaa tcatgcgcgc tgcaattttc agcgccagtg ccatcgtatc catcgcgccg
     2041 atatgaccgt aaaacagatc atatttatca gtactttgac gacgtacttt ggcatcgaag
     2101 ttcagaccac cggtggtgaa accgcctgct ttgagaattt catacatcac cagcgcattc
     2161 tcttccacac tgttcgggaa ctggtcggtg tcccagccca gttgcgcatc gccacggttg
     2221 gcgtcgacag aaccgaacag gccaagcgca atggcggtgg ctatttcatg atggaaagag
     2281 tgacctgcca gcgtcgcgtg gttagcttca atgttcagtt taatctcttt ttccagacca
     2341 aactgtttca ggaagccata gaccgtcgcg gcatcgtaat catattgatg tttagtcggt
     2401 tcttgcggtt tcggttcgat aagcaacgtg ccctggaagc cgattttatg tttatgctca
     2461 accaccatct gcataaagcg gcccagttgt tcacgctcct gacgcaagtc ggtatttaac
     2521 agcgtttcgt aaccttcacg accaccccac aggacatagt tttcaccgcc caatttatgg
     2581 gttgcttcca tcgctgtaac aacttgcgtt gccgcccagc tgaagacttc aggatctggg
     2641 ttcgtcgccg cacccgcgcc ata
//
LOCUS       DAJVRF010000259         2661 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJVRF010000259 DAJVRF010000000
VERSION     DAJVRF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.263"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..1335
                     /locus_tag="OQN13_003987"
     CDS             <1..1335
                     /locus_tag="OQN13_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCS1617188.1"
                     /translation="ACDNEFGAEVYSGATTEKQAWEVFRPARLMCKRTPMLTEAFGIE
                     VNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHATDALYTTMLTGMGARRQP
                     LMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIYTVDEGDDWTGPQVLEKA
                     NPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSARSAYFNLVSWQSCEDKS
                     LTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYYSVAPRFWVPYDTVYSVE
                     KNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAANKISPVSESPIDPFGATG
                     LSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHDGNPIMTWCIGNVVGKNM
                     PGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLSDHIESYGIRSL"
     gene            1347..1529
                     /locus_tag="OQN13_003988"
     CDS             1347..1529
                     /locus_tag="OQN13_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617189.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            1529..2661
                     /locus_tag="OQN13_003989"
                     /pseudo
     CDS             1529..2661
                     /locus_tag="OQN13_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          613 a          650 c          803 g          595 t
ORIGIN      
        1 gcctgtgata acgagtttgg cgcggaagtg tattccggtg ccacgacaga gaaacaggcg
       61 tgggaagtct ttcgcccggc gcgactgatg tgtaaacgca cacccatgct gacggaagcg
      121 ttcgggattg aggttaacgc ctcaaacatg aaccgtccgg aggatggcgc gcggtttgaa
      181 ccgctgatcg gcaacccagg tgatggttca tcaccccact gtgccgtggt tgatgaatat
      241 cacgagcatg ccaccgatgc gctttatacc acaatgctta ccgggatggg ggcgcgacgt
      301 cagccactga tgtgggccat caccaccgcc gggtacaaca ttgaggggcc gtgctacgac
      361 aagcggcggg aagtcatcga gatgctcaac ggctcggtgc ctaacgatga actgttcggg
      421 atcatctata ccgttgatga aggtgacgac tggaccggcc cgcaggtgct ggaaaaagcc
      481 aatccaaata ttggcgtgtc ggtttatcgc gaatttttgt taagtcagca gcagcgtgcg
      541 aaaaataacg cccgtctggc aaacgtcttt aaaacaaaac acctcaatat ctgggtgtcg
      601 gcgcgttcgg cgtatttcaa cttggtgagc tggcagagct gcgaggataa atcactgacc
      661 cttgagcagt tcgaggggca gccgtgcatt ctggcctttg acctggcgcg taagctggat
      721 atgaacagca tggcgcgact ttatacccgc gagattgacg gtaaaacgca ttactacagt
      781 gtggccccgc gtttctgggt accgtatgac acggtgtaca gcgtcgagaa aaatgaagat
      841 cgacggacag ccgaacgctt tcagaaatgg gtggaaatgg gcgttctgac cgttaccgat
      901 ggtgcggagg tggattatcg ctacatcctc gaggaggcca aagcggcgaa caaaatcagc
      961 ccggtcagtg agtcacccat cgaccccttc ggggcgaccg ggttgtcaca tgaccttgct
     1021 gatgaagacc tgaatcccgt cactatcgtc cagaacttca ccaatatgtc cgatccgatg
     1081 aaagagctgg aagcagcgat tgaatcggga cgttttcatc atgacggcaa tcccatcatg
     1141 acctggtgta tcggcaacgt ggtcggcaaa aacatgccgg gtaacgatga tgtggtgaaa
     1201 cccgtcaaag agcaggcgga aaacaaaatc gatggtgcag ttgcgctgat tatggcggtt
     1261 ggcagagcca tgctgtacga gaaagaagac acgctgtctg accacattga gtcctacggg
     1321 atccgctcgc tttaactgag gtaattatga tcatgctgat tctcgcgcct ctggtgggcg
     1381 tgctgggtgc gcttttgctg gcgtatggtg cctggctgat ttatcccccg gcgggttttg
     1441 ttgttgccgg ggcgctgtgc ctgttctggt cgtggctggt ggcgcgatat ctcgaccgta
     1501 cacggcagtc tgtcggcgga ggtaaatagt gttcttttcg ggattatttc aacgaaaaag
     1561 cgatgtgccg gtgaccacgc cagcagaact gctgatgcta tcgggctgtc atacgacacc
     1621 tataccggaa agcggatcag tagccagcgg gccatgcgcc tgacggcggt ctattcttgc
     1681 gtcagggtgc tggcagagtc tgttggtatg ctgccctgca gtctctacaa aatcagcggt
     1741 accctgaaaa cacgggcggt ggatgaacga ctgcataagt tggtttcggc aaaacctaat
     1801 ggctacatga caccgcagga attctgggag ctggttattg tctgcctgtg tctacggggg
     1861 aatttttacg cctacaaggt aaaggcactg ggagaagtgg tggagcttct tccgatagat
     1921 ccgggctgtg tggaaccgaa gctgaacagc cagtggcagc cggtttatca ggtgacgttt
     1981 ccggatggtt ccgtggatgt gttgacccag aatgaaatct ggcatgtgcg caccctgacg
     2041 ctggatggac ttgtcgggct gaatcccatt gcgtatgcgc gtgaggccat ttcactggca
     2101 gcggcaaccg aggagcacgg tgccaggttg tttagtaatg gtgcggtgac atccggtgtg
     2161 ttgcgtacgg aacaaaagct cacgccggat gcttacgagc gtctgaagaa agattttgag
     2221 gagtgtcaca cggggcttgg caatgctcac cgcccgatga ttctggaaat ggggctggac
     2281 tggaagtcga tggcgctgaa cgccgaggac agtcagttcc tggaaacccg caagtttcag
     2341 ctggaagaaa tctgtcgtct gttccgggtg ccgttgcaca tggtgcagaa caccgatcgc
     2401 gccaccttca acaatatcga agagctgggg ctgggattta tcaactattc actggtgccg
     2461 tatctgaccc gcatcgaaca gcggatcaac accggactgg tacgaaaaag taagcagggc
     2521 gtttattacg ccaaatttaa cgccggggcg ttactgcgcg gggatatgaa gtcccgtttt
     2581 gaagcctacg ccaccgggat caactgggga atttactctt ccaatgactg ccgcgacctg
     2641 gaagatatga atccgcgtcc c
//
LOCUS       DAJVRF010000260         2640 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJVRF010000260 DAJVRF010000000
VERSION     DAJVRF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.264"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..778)
                     /gene="tauC"
                     /locus_tag="OQN13_003990"
     CDS             complement(<1..778)
                     /gene="tauC"
                     /locus_tag="OQN13_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCS1617190.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELG"
     gene            complement(775..1542)
                     /gene="tauB"
                     /locus_tag="OQN13_003991"
     CDS             complement(775..1542)
                     /gene="tauB"
                     /locus_tag="OQN13_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCS1617191.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(1555..2517)
                     /gene="tauA"
                     /locus_tag="OQN13_003992"
     CDS             complement(1555..2517)
                     /gene="tauA"
                     /locus_tag="OQN13_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1617192.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT          595 a          808 c          668 g          569 t
ORIGIN      
        1 gacccagttc taaaagaaag gcgataatcg caatcaccgc gatccccgcc agtaccacgt
       61 cagtcgcgag aaattcaccc gctgactgaa ccataaatcc taaaccgcgt gtcgcggcaa
      121 tcagctccgc cgctaccagc gtagaccagc ccacacccag accaatgcgt aatccggtga
      181 ggatttccgg cagcgcgccg ggcaaaatga caaaccacag cacctgcgca cggctggcac
      241 ccagcgactg ggcggcacga atgcgaacct gctgcgcgct tttcaccccc gccagcaccg
      301 acatcgccac cggtgcaaaa atcgctaaat agatcaataa gatcttcgag gtttcaccaa
      361 taccaaacca gatcaccatc agcggcaaat aagccagcgg cggcaccgga cgataaagtt
      421 cgattaccgg atccagaatg ccgcgcaccg tcgggcttag ccccatggcg atcccgaccg
      481 gaataccgat caccaccgcc gcgagtagcg ccagcacaat gcgcgtcagg ctggctgcca
      541 gatgctgcca cagcgtggcg tccataaatc cctgcggtcc ggcaatggtg agcagttttg
      601 ccagtacttg ttgcggcggc ggcaaaaaca gcgggctaat cagttgcagc gccgccaccg
      661 tccaccatac agtgagtaaa accgctaacg tgccaatgct taaggtcacc tgacgcgaga
      721 gcggccagcg ccatttcagc cgccgcgaat gcagtttttc attaatgagc acactcatga
      781 gaacgcctcc cgttgctcaa atacgcggct taaaacatat tcgcgcatgg cgatgaattg
      841 tggatcggac ttgatgctgc ggctcgactc tcccgcaaca aagcggcgag caaagttgag
      901 cggcagccgc tccagcacac ggccagggcc ggatgaaagc agaaccagtt cagtcgccat
      961 aaacaccgct tcttctatat cgtgggtaat caacagcacc tgcttgcccg tctcctgcca
     1021 gagtttcagc agcagagttt gcatctggtc gcgggtgaag gcgtccagcg caccaaacgg
     1081 ttcgtcgagt aataacagct ggggattcgc cgccagcgcg cgggcaatcc ccacccgctg
     1141 acgttgacca ccggaaagct gccagatata gcgtttttct gcgccttcca gccccacttt
     1201 tttcagcatc tggtgcgcga tttccagtcg ctgcattttc tctatacctg ccaattgcag
     1261 gccgaacgcc acgttgtcct gtacattgcg ccacggtagt agcccttcat tctgaaaaac
     1321 caccccgcgc tctgctcccg gtccctcaat acgcttaccc gccagttgaa tgctgccatg
     1381 ctgataaggc acaaaaccgg caatcagatt cagcagggtg gttttaccgc agccggacgg
     1441 ccccagcacc accagtagct cgccgctttc cagcgtcagg ttgatatctt ccagtgccgg
     1501 tttgccgcca taatcggcat agagatgaga gatttgcagc atctgcgcct ccttttattg
     1561 cacgaagcgc gaggtaacgt actggctgta atcattcgct acagccggga ccttgccctg
     1621 ctctttcaaa aactgcgcgg tgtcgatgat tgctttgttc accggtccgg tcagttctgc
     1681 cgtttgttgc tgcggcgtca gataggtatt ccccttcacc agccccggaa cgtcaccttc
     1741 cggcacgccg cttaaacgcg ccagtttgct gatgttttcc ggctgtttca gccacgcgtc
     1801 tgggttagca atgtacggtt gctgagcatc gatggcgctt ttagcgaacg ctttcacgac
     1861 ctcaggatgt ttctcggcaa aatctttgcg caccacccag acgtccagcg ttggcgcacc
     1921 ccactgcccg acctgttcag aatcggtcaa caccttgccg tctttttcca gggcgttaac
     1981 cgccggtgcc cagacataag caccatcaat atcaccccgc tgccatgcgg cgataatcgc
     2041 gggcggctgc aggttcacaa tctccacttg ccctggttta atcccccagt gtttcagcgc
     2101 cgccagcagg ctgtagtggg tggtggagat aaacggtacg gcaatgcgtt tgccaatcaa
     2161 atcttcgggt ttgctgatag ttttctttac caccagcgct tcggagttac ccagttttga
     2221 cgccagcaag aagacttcaa tcggcacctg ttggctggct gcaaccgcta atgggctgga
     2281 accgaggttg ccgatttgca cgtcgccaga agccagcgcc cgcacgatgc tggctccgct
     2341 gtcaaactta cgccagtcca cgctcgcccc gctctcttta gcaaaggtgt tgtcggcctg
     2401 agccactttc gccggttcgg ctgaggtttg atacgccacg gtgacgttca ccgcctgtgc
     2461 ctgaaaagcg atgaatgcca gtgcggcaag aagtgtgtta cgcgatgaaa ttgccatgat
     2521 tgtctgctcc cctgtcttgt tatgggagca gtattcagga ataaaaacat tcattaaaag
     2581 aattagtcgt tatcgcacag atgattttat tcttagcaaa aaaacggcaa aggaaagatt
//
LOCUS       DAJVRF010000261         2618 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJVRF010000261 DAJVRF010000000
VERSION     DAJVRF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.265"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            48..203
                     /gene="hokD"
                     /locus_tag="OQN13_003993"
     CDS             48..203
                     /gene="hokD"
                     /locus_tag="OQN13_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCS1617193.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            507..779
                     /locus_tag="OQN13_003994"
     CDS             507..779
                     /locus_tag="OQN13_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617194.1"
                     /translation="MPSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEWLI
                     QCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            853..1839
                     /locus_tag="OQN13_003995"
     CDS             853..1839
                     /locus_tag="OQN13_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS1617195.1"
                     /translation="MSAFHNGRILVEPEPKSMRNLPSGVVPAARQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSVYSESDIIPGEQTATSILKQRTKNIALLPHVHQQQNPPQEKTVVS
                     IAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTK
                     ADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            1840..2220
                     /locus_tag="OQN13_003996"
     CDS             1840..2220
                     /locus_tag="OQN13_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCS1617196.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            2217..>2618
                     /locus_tag="OQN13_003997"
     CDS             2217..>2618
                     /locus_tag="OQN13_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS1617197.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNK"
BASE COUNT          683 a          620 c          719 g          596 t
ORIGIN      
        1 aatctaggtt agcctcttac gtgccgaaag gcaaggagaa gcaggctatg aagcagcaaa
       61 aggcgatgtt aatcgccctg atcgtcatct gtttaaccgt catagtgacg gcactggtaa
      121 cgaggaaaga cctctgcgag gtacgaattc gaaccggcca gacggaggtc gctgtcttca
      181 cagcttacga acctgaggag taagagacca ggcgggggag aaatccctcg ccacctctga
      241 tgggtcaggc atcctcaatg cacccacact taacccgctt cggcgggttt ttgtttttat
      301 tttcaacgcg tttgaagttt tagatggtgc cggaatagaa tcaaaaatac ttaagtagcg
      361 cgcagggaga agagggatgg accccgaaca ggggagtgct atttatctgg aaggattctg
      421 ttgatgaaaa tcgaagaatt acgtgaaatt tttagtgaag atggcctcta tactgtgcgc
      481 gttgagaatg gcgctattgt cagccactgc cttccagact acacaattct gataattcag
      541 ccgtctttgc cagcaggcac gggcggcgct ctcacgcatt taaatctgat tggtaccagc
      601 atcccccatg cactgaagaa caggccgaat ggctcattca gtgttaccgc aggcgcggat
      661 gcgaggttaa aaaagccctt agcctcgact accgtcactg gataatctcc gtcaggctcc
      721 cttactccga acggccaacg cgtccgtccc gcacattcca gcaacggatc tggaggtaat
      781 gtgcgggtat tacttcgacc tgttctggta ccggaactcg gtctggttat cgttaagcca
      841 ggccgtgaat caatgtcagc attccataac ggcagaatac tggtggagcc ggaaccaaaa
      901 agcatgcgta atctgccgtc cggggtcgtt cctgccgctc gccagccgct ggtggaagac
      961 aaaacattgc tgccgttttt cagtaacgca cgggtgattc gtgctgctgg tggtgctggt
     1021 gcattgtctg actggctgtt gcgccatatt aaatcctgcc agtggccaca cggcgattat
     1081 catcacagcg aaaccgtcat tcaccgttat ggtaccggcg caatggtgtt gtgctggcac
     1141 tgcgacaacc agctgcgtga ccagacatcc gaatcactcg agcaacttgc tcatcaaaac
     1201 ctgtcagcat ggatgattga cgtcatcggt cacgcaataa gcggtacgca ggagcgtgaa
     1261 ttatctctgg ctgaattatc ctggtgggcg gtccgcaatc aggtggcgga cgcgctaccg
     1321 gaagcggtat tacgtcgttc gctggggttg cgtgcggaaa aaattcgctc tgtgtacagt
     1381 gaaagcgaca tcataccggg agagcagaca gccaccagca tactgaagca gcgcacaaaa
     1441 aatattgcgt tactgcctca cgtccaccag caacagaacc caccacagga aaagacggtg
     1501 gtcagcattg ccgttgatcc ggagtcaccg gctcagtatc tccagcgcca gaaatcacaa
     1561 cgggaagaga tgcctgtata cacgcgctgg gtaaaaacgc agaaatgcat gacgtgtggc
     1621 aatcaggcag atgatccgca tcacatcatt ggtcatggac tgggagggat gggaacaaag
     1681 gctgatgatt tgtttgttat tccgttatgc cgtaaatgcc gtaacgaact acatgccgga
     1741 gtaaaagatt ttgaagagaa acacggcagt cagctgttgt tgctgattcg ttttttaatg
     1801 cacgcgagaa attcgggtgt tctgaagtgg aaagcataaa tgaccgagcg catagaattt
     1861 gttttacctt acccgccgac ggtgaacact tactggcgtc gtcgtggcag cacatatttt
     1921 gtatcaaaag ccggggagcg ttatcgccgg gcagtggcgc ttattgttcg ccagcagcgg
     1981 ctgaaattaa gcctgtccgg aaggttggca ataaaaatta ttgcagaacc accggataag
     2041 cgccgccgtg acctggacaa tattctgaaa gcgccgctgg atgcgctgac gcatgcgggg
     2101 ttgctaatgg acgatgagca gtttgatgaa atcaatatcg ttcgtgctca gccagtatct
     2161 ggtggacgtc tgggggtgaa gatttatccc ataatgcttg aagggcaggt caaaaaatga
     2221 aactggaaga tttaccgaaa tactactccc caaaatcccc cggcctgact gatgcatcgg
     2281 cctcaacgtc gaaagatgcg ctgagtatca ctgatgtgat ggccgcgcag ggcatgacac
     2341 agaatcgggc tgagatgggg gtttctgcgt tccttgggaa aatgggcatt agtatgaatg
     2401 acagagagcg ggcaacagaa ttgctgacag aatatgcact cagtcggtgt gatcgcgtgg
     2461 cggcgttaag aaaactcccg gcagaaataa aaccggcagt gatgcgtatt atggcttcgt
     2521 atgcgtttga agattatgcc cgtagcgcgg cgagcaaaaa acagtgcccc tgctgtcacg
     2581 gaaaaaaatt tattgaaagc gaggttttta caaacaag
//
LOCUS       DAJVRF010000262         2450 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJVRF010000262 DAJVRF010000000
VERSION     DAJVRF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.266"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..1692
                     /locus_tag="OQN13_003998"
     CDS             <1..1692
                     /locus_tag="OQN13_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCS1617198.1"
                     /translation="AIVDNGAHFDGDRRGTVNGVTPPAVQHLTAEVTADSGEYQVLAR
                     WDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQLALGNYSLTVRAVNAR
                     GQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDPTVQFEFWFSEKRIADI
                     RQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKSAFVEAIGRASDDAEGY
                     LNFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSNEITQTVNKKLENQSAA
                     IQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIENTPAGMQSQVLLAADRI
                     AMINPANGNTKPMFVGQGDQIFMNDVFLKCLTAPTITSGGNPPAFSLTPGGRLTAKNA
                     DISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVGKPFPRDSRAPERWPSG
                     TITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLIVKKNGAEIYNRTTLDN
                     TLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLVVVMKKATAGITIS"
     gene            1760..2359
                     /locus_tag="OQN13_003999"
     CDS             1760..2359
                     /locus_tag="OQN13_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCS1617199.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDGGRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
BASE COUNT          593 a          570 c          736 g          551 t
ORIGIN      
        1 gccatcgtgg ataacggggc acactttgac ggtgaccggc gcggcacggt gaatggtgtc
       61 acgccgccag cggtgcagca tctgaccgcc gaagtcaccg cagacagcgg ggaatatcag
      121 gtgctggcgc gatgggacac gccgaaggtg gtgaagggtg tgagcttcct gcttcgcctg
      181 accgtggcag cggacgacgg cagtgagcgg ctggtcagca cagccaggac gacggaaacc
      241 acataccgct tcaggcagct ggcgctgggg aattacagtc tgacagtccg ggcggtaaat
      301 gcccgggggc agcagggcga tccggcgtcg gtatcgttcc ggattgcggc accggcagcg
      361 cctgtcacta ttgaactgat accggggtat tttcagataa cggcggtccc gaaactggct
      421 gtatatgacc cgacggtgca gtttgagttc tggttttcgg aaaagcggat tgcggatatc
      481 aggcaggttg aaaccagcgc ccgctatctt ggcacggcgc tgtactggat agccgccagt
      541 atcaatatca ggccgggcca tgattattat ttttacgttc gcagtgtgaa caccgttggc
      601 aaatcggcat ttgtggaggc catcggtcgg gcgagcgatg atgcagaagg ttatctgaat
      661 ttttataaag ggttgatcaa taaaacgcat ctcggcaagg agttgtggac gcagattgat
      721 aacggtcagc ttgcgccgga cctgactgaa atcaggacgt ccattacgaa tgtcagcaat
      781 gaaatcacgc aaaccgtcaa taaaaaactg gaaaatcaga gtgctgcaat ccagcagata
      841 cagaaagttc aggttgatac aaataataac ctgaacagca tgtgggctgt gaagctgcaa
      901 cagatgaagg acggacgcct ttatattgcg ggtatcggtg ccggtattga gaatacgcca
      961 gcaggaatgc agagtcaggt gctgctggcg gcagacagga ttgcgatgat taatcctgcg
     1021 aatggcaaca caaagccgat gtttgttggt cagggcgatc agatattcat gaacgacgtg
     1081 ttcctgaaat gcctgacggc tccgaccatt accagcggcg gtaatcctcc ggcattttcc
     1141 ctgacacctg gcggacggct gacggcgaaa aatgccgata tcagcggtaa cgtgaacgcg
     1201 aactccggga cgctcaacaa cgtcacgatt aataagaact gtcgggttct gggaaaattg
     1261 tccgcgaacc agattgaagg cgatctcgtt aaaacagtgg gcaaaccttt cccacgggac
     1321 tcccgggcac cggagaggtg gccatcaggg accattaccg tcagggttta tgacgatcag
     1381 ccgtttgatc ggcaaattgt tattcccgcg gtggcgtttc gcggtgctaa acatgagcgg
     1441 aagaataaca atatttattc gtcatgccgc ctgatagtga agaaaaacgg tgctgaaatt
     1501 tataaccgaa cgaccctgga taatacgctg atatatacgg gtgttattga tatgcctgcc
     1561 ggtcacggtc acatgacgct ggagttttct gtatcggcat ggctggtaaa tggctggtat
     1621 cccacagcaa gtatcagcga tttgctggtt gtggtgatga agaaagccac tgcaggcatc
     1681 acgattagct gaattttata acccagatac gggcaccaga aatggtgcct tttttattgc
     1741 agaaaagcga gaggtaatta tgcgtaaagt ttgtgcagcc attttgtccg cagccatctg
     1801 tctggccgta tccggtgcgc ctgcatgggc gtctgaacat cagtccacgc tgagcgccgg
     1861 gtatcttcag acccatactg atatgccagg cagcgatgac ctgaagggca ttaacgtgaa
     1921 ataccgttat gagtttacgg acacgctggg gctgattacg tcattcagtt atgccaacgc
     1981 tgaagatgag caaaaaacgc attacagcga tacccgctgg catgaggatt ccgtgcgtaa
     2041 ccgctggttc agcgtgatgg cggggccgtc tgtgcgcgtg aatgaatggt tcagcgcgta
     2101 tgcgatggcg ggcgtggctt acagccgtgt gtcgaccttc tccggggatt atctccgcgt
     2161 aactgacaac aaggggaaaa cgcacgatgt gctgaccgga agtgatggcg gtcgccacag
     2221 caacacgtct ctggcgtggg gggctggcgt gcagtttaac ccgaccgaat ccgtggccat
     2281 tgaccttgct tatgaaggtt ccggcagtgg tgactggcgc actgacggtt tcatcgtggg
     2341 tgtcggttat aagttctgat tagccaggta acacagtgtt atgactgccc gctggttcag
     2401 gcgggctttt tgtggggtga atatggcagt aaagatttca ggtgtactga
//
LOCUS       DAJVRF010000263         2436 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJVRF010000263 DAJVRF010000000
VERSION     DAJVRF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.267"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(25..315)
                     /locus_tag="OQN13_004000"
     CDS             complement(25..315)
                     /locus_tag="OQN13_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCS1617200.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(305..742)
                     /gene="ratA"
                     /locus_tag="OQN13_004001"
     CDS             complement(305..742)
                     /gene="ratA"
                     /locus_tag="OQN13_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCS1617201.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            913..1395
                     /gene="smpB"
                     /locus_tag="OQN13_004002"
     CDS             913..1395
                     /gene="smpB"
                     /locus_tag="OQN13_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCS1617202.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1610..1972
                     /gene="ssrA"
                     /locus_tag="OQN13_004003"
     tmRNA           1610..1972
                     /gene="ssrA"
                     /locus_tag="OQN13_004003"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2171..>2436)
                     /locus_tag="OQN13_004004"
     CDS             complement(2171..>2436)
                     /locus_tag="OQN13_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCS1617203.1"
                     /translation="NAGPDSLGRLSPVVMLAKSVTAQTHMAWGDADNQQVKLSMPELE
                     ELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLGTIRAFDVT"
BASE COUNT          663 a          652 c          547 g          574 t
ORIGIN      
        1 caatgagcac cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg
       61 aagctctttc ggatcggcaa tgagagggcg ataaatctcc acccgatcgc catcatgcac
      121 actatcgctt agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat
      181 atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg tcgcgccctc
      241 ctgcagcgtc acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc
      301 aattttacct agcactgtaa acctctttcg cacgaaccgt aaaagcctgg accatattag
      361 acgccagctc tttaaacacg cgaccaaagg cgagttcaat caacttattg gtaaactcaa
      421 agtcgagatg aaactcgata cgacacgcct cctggctcag cggcgtaaac ttccatccac
      481 caatcaattt cttgaacggc ccatccacca gattcataag aatactttgg ttactggtca
      541 actggttgcg ggtagtaaac gttttgctga tcccagcctt agagacatct accgccgcag
      601 tcatctgccc aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag
      661 gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg
      721 cggtccggct aatctgaggc ataactattt ccatcaacaa aaatccaaca aataatatca
      781 tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag
      841 atagagcctt gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga
      901 ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt
      961 aacaagcgcg cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg
     1021 caaggctggg aagttaaatc cctgcgcgca ggaaaagcca atatcagcga cagctacgtc
     1081 cttctgcgtg acggagaggc atttctgttt ggcgctaaca tcacgccaat ggccgtggcc
     1141 tccacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa
     1201 ctggactcat tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg
     1261 tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac
     1321 gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcacg tatcatgaaa
     1381 aacgcccacc gttaaacctg cactccaatt attgaccagt tcctcaccgc gcctccctct
     1441 ccggcggcgc gaatgaacat cttattggct atcacatccg acacaaatgt tgccatccca
     1501 ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga taacgccatt
     1561 gaggctggtc atggcgctca taaatctggt atacttacct ttacacattg gggctgattc
     1621 tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa
     1681 aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct
     1741 tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa
     1801 aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt
     1861 gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt
     1921 accgaggatg taggaatttc ggacgcgggt tcaactcccg ccagctccac cacttttgac
     1981 aggactgcaa ccggacagag actataaaaa cagtcactta cggacactga ccagacagta
     2041 agcggaccag aaaaagacaa aaatatacac gcaaaatgca cgtgcatttc aaaaaagcct
     2101 ctgattatga agtcagaggc ttttctagtt gtaaataagg gtaacaattt aacacgatgc
     2161 agcttaccaa ttacgtaaca tcaaacgctc taattgttcc caaatcctcc agaccactca
     2221 gctcctcttt catttcacgc tgacggcgat aaatctcatc gttgcgatcg acctgcgcct
     2281 gcaccattgc tgccgccagt tcttccagtt ccggcatcga cagtttcacc tgctgattat
     2341 cggcatcacc ccacgccata tgcgtttgtg ctgtgacaga ttttgccagc atgactaccg
     2401 gggacaggcg gcccagtgag tcggggccag cattcc
//
LOCUS       DAJVRF010000264         2416 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJVRF010000264 DAJVRF010000000
VERSION     DAJVRF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.268"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..229)
                     /locus_tag="OQN13_004005"
     CDS             complement(<1..229)
                     /locus_tag="OQN13_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HCS1617204.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGD"
     gene            complement(234..449)
                     /locus_tag="OQN13_004006"
     CDS             complement(234..449)
                     /locus_tag="OQN13_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCS1617205.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(600..>2416)
                     /locus_tag="OQN13_004007"
     CDS             complement(600..>2416)
                     /locus_tag="OQN13_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCS1617206.1"
                     /translation="PSDLAKRLTQKLKEGWQPFGSPVAITPYTLMQAIAAEGDVVVSG
                     ATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRNTVTPGGEVCVFND
                     IIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPYIPNNAGILLVPCC
                     RGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAALQKNPKNVLLAICW
                     MQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASVPWICGDTTYAWKQ
                     EHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPDIAGSGYYGSASRT
                     NKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKAPEIKPSPGGDTPS
                     GPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQSNKAWSLTRPVDDA
                     VSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTGTGNPFLMSFFTQT
                     TDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPKLNGVAGPAFQVIK
                     DSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
BASE COUNT          550 a          695 c          576 g          595 t
ORIGIN      
        1 cgtccccttc acataacccg gcagcaacat ccaggaagac ctgtctgatg ctccttctgg
       61 ctgctgcctc ataaaactcc agcgcggcac cttcaacacg gtccagcgag atgtccaggt
      121 caaaaatttc accgtcaaag cgttttttgt cccgtaacgc taaagttacc gtaactttat
      181 tctcaaaatt gcggatccct ttcacaatca gttcatagtt ttgagtcatt gaattactct
      241 ccccgtgcag ccttacgacg gtcctctctg attttgaaat acaggttagt cagatatgtc
      301 agcagcccaa acagcagact ccccagcacg cctattgccg cccactgaga cggggaaacc
      361 ctgtccagca actgcaggaa ccagtagccc gttcccaccg ctgacgtggt gtatgacaca
      421 cctgttgtga ttttttccat ctggtacata ccccgtctcc cgttatccgg aagctgacaa
      481 caataaaaaa agccaccagt taagtactga tggctctgat aactcatgca ggcatctcag
      541 acgacccact gacactaccg gtgagtttaa cgataccttc catttggctg gctcactttt
      601 tatgatgatg ccggtgcatt tatctccagc accagacttt ctatctcaac gccatacgct
      661 gcatttttgg taatatccgt cagcgtcagc gcattcagcc ccagtgtcag actgtctttt
      721 atgacctgga atgccgggcc agccactcca ttcagtttcg gagtaaccgt ggcactgccg
      781 gcggtgaaca ccagctccag cgtcagccag tcgttactgt aattcccgaa ctcgcccaac
      841 tttgtgtttc ctgctttctt gtgatgcatc agattcagtt tgccgtctgt ggtctgggtg
      901 aagaacgaca tcaggaacgg gttaccagtc ccggtcatcg ccacgacgtc aggtaacgct
      961 acatcggtat acagataaat tcccagaccg aactggttgt tggtcagtgc gcctgacagt
     1021 cgaaacttac agctcagtct gccaccccgt gtcagcaggg agactgcgtc atccaccggg
     1081 cgcgtcaggg accaggcttt attgctctgc ttggtgatct taaatacacc atctgacaac
     1141 tgaactccgc cattcttaat gctccagccc tgcgcagcag cgtctccggc tgtcggcaac
     1201 agggagattg tgcgtatgga tgcatcttca gacggccccg atggcgtgtc gccgccgggc
     1261 gagggtttga tttccggtgc cttaccacta atgaaggcta aggtgcgacc ggctacgttc
     1321 agaatagcag ttgccatacg atcgggaata atgccacgac gcgcccatga gctgaaatgc
     1381 gtcgggcgat ttgatgatac ccagtttttg ttcgttcggg atgccgaacc gtaataacca
     1441 gacccggcaa tatcaggatc ttctgacggg ttgtttgtcg gtgtattaac tccgctacca
     1501 tcggtcataa agggaacaaa ataaatctgc tgggattctt tacctttata tgcaccatat
     1561 accacttcat attgcgtacc gtgttcttgt ttccacgcgt atgtcgtgtc gccacaaatc
     1621 caggggactg atgccggact tccaccgtga cactgcgccg ccagcccggc aaggtcagca
     1681 cggaactgct gtaccattgc aagaaatgct gctggctgct gggcgtaact ggcattcgtc
     1741 atatcgaatt ccccctgcat ccagcatatc gccagcaaaa cgtttttcgg gtttttctgc
     1801 aatgctgcct tcgtgcggaa aagcagatcc tgatataacg gcttacccac tccccagcga
     1861 gccgaatcct gactggcccc cgtggactcg ctgaatgtcc cctccgtgcc ctgggtgaat
     1921 gccgaaccac cacgacagca tggtaccagc aggatccccg cattattagg gatatacgga
     1981 agcagttttt tggcaatatg taagccctgt ccgacacagc cgtactgccc tttgctcagg
     2041 tcagcccggg gatggttaat cgtactcata tcctgaacat catgcagaca atggtcagca
     2101 ggaatgatgt cgttaaatac gcatacttca ccaccgggag tcactgtgtt acgacgggcc
     2161 agttgcttaa tgcgcggatg gggcgcatcg tatgaatccg gaagcggaag accttcaccg
     2221 taagccatgg cattggattg cccggccagt acgatgacgt agtaccactc cggctcagtt
     2281 gcaccactga cgaccacatc accttctgct gcaatcgcct gcatcagggt ataaggggtt
     2341 atggccaccg gactaccaaa cggctgccag ccctccttca gtttttgtgt cagtcgtttc
     2401 gcaaggtctg acggcg
//
LOCUS       DAJVRF010000265         2414 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJVRF010000265 DAJVRF010000000
VERSION     DAJVRF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.269"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..394
                     /locus_tag="OQN13_004008"
     CDS             <1..394
                     /locus_tag="OQN13_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1617207.1"
                     /translation="VFPSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTI
                     EEELAWHYIMLASRKAHELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYD
                     KQFWEHYSKKCRWPIRNVPWGASSVKYS"
     gene            complement(511..1854)
                     /gene="gdhA"
                     /locus_tag="OQN13_004009"
     CDS             complement(511..1854)
                     /gene="gdhA"
                     /locus_tag="OQN13_004009"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCS1617208.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            2090..2362
                     /locus_tag="OQN13_004010"
     CDS             2090..2362
                     /locus_tag="OQN13_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCS1617209.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(2328..>2414)
                     /locus_tag="OQN13_004011"
     CDS             complement(2328..>2414)
                     /locus_tag="OQN13_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine (deoxy)nucleoside triphosphate
                     diphosphatase"
                     /protein_id="HCS1617210.1"
                     /translation="EALQYPLAPADIPLLEAFMALRAARAAD"
BASE COUNT          618 a          615 c          589 g          592 t
ORIGIN      
        1 tgtttttccc tcagactata tctctgattg cgctaataat cgctccggta aatcctcggc
       61 gaagctaata tgggcggcgg aattatcatg gatgacctcg atatcaagca cagcttttca
      121 aaacgggaca attgaagaag aactggcctg gcattatata atgcttgctt ctcgaaaggc
      181 gcacgagttg ttcgaaagcg aagaagatta tcaaaaaaat agtctaatgg ggtttcttta
      241 ctggcatatc tgctgctatc gcagaaagtt aacggatgct gaacttgaag cgtgttatcg
      301 ttacgacaag caattttggg agcactacag taagaaatgc cgttggccca taagaaatgt
      361 tccctgggga gcatcatccg ttaaatactc ataaacgcct gaaattttgc cgggggcgct
      421 gcgcttgccc ggcctacatt agagcgtaac tgcaatgaat tgtgcccatt tgtaggcctg
      481 ataagcgtag cgccatcagg catttacaac ttaaatcaca ccctgcgcca gcatcgcatc
      541 ggcaaccttc acaaaaccgg caatgttcgc gccctgcacg tagttggttt gctcaccttc
      601 accaccatgc tcaacacagg catggtggat atccagcatg atgtgatgca aacgtgcgtc
      661 aactttctcg gctttccagc ccaggcgcgc agcgttttgt gccatttcca ggcccgacgt
      721 tgctacaccg ccagcattag ccgctttacc cggtgcaaac agtacgcctg cctgctggaa
      781 cagttcagtc gcttcgatgg tggtcggcat atttgcccct tcagcgacgg ctttaacgcc
      841 attggcgata agctgatgcg cggcgtcaac atccagttcg ttctgggtgg cgcaaggcag
      901 ggcgatatca accggtacag accacggctg ttggccttcg agatagacca gaccaaattc
      961 tttggcgtaa tctgccactc gaccatcgcg gctggctttg atttcgataa gacgtgccag
     1021 tttctctttc gtgaatccgc tttcatcaac tacagtgccg ctggagtctg acgcagtgat
     1081 cacacgagca ccaaattcca tcgctttttc gatagcgtac tgggcgacgt tgccggagcc
     1141 agaaacggaa acgcgcatcc cttcaaaacc cataccgtgg cgttttaaca ttgcttctgt
     1201 gaaataaacc agaccgtagc cggtagcttc cgggcgaata agactaccgc caaatgaaag
     1261 gcccttaccg gtgaagacgc aggcggtatt gttggagagc tttttcatca tccccgccat
     1321 aaagccgact tcacgaccac caacgccgat atcacctgcc ggaacgtcgg tatccgcgcc
     1381 cagatgacga taaagctcgg tcatcagcgc ctggcaaaaa cgcatcactt caccttcgct
     1441 ttttcctttc ggatcgaaat cgctgccgcc tttaccaccg cccatcggca gggtagtcag
     1501 ggcatttttg aaggtttgtt caaagccgag gaatttgaga atggaaaggt taactgacgg
     1561 atggaagcgc ataccgcctt tgtacgggcc gatggcagag ctgaactgca cacgccatgc
     1621 acggttgacc tgtatctggt tgcgatcatc aacccatacc acgcgaaact ggatcacgcg
     1681 ctccggttca accagacgct ccagtaatga catctggcga tattttggat tttgttcaag
     1741 aaaaggccag agtgtggtca ttacttcacg aacggcttgc gcgaactcgg tttgattcgg
     1801 gtcgcgcttt tggacatggt tgaggaatga ctccagagaa tatgtctgat ccatagatat
     1861 aaaaccctta tatattaata cgattgtgct tatgttgttt atgtcatgtg cttttgcagt
     1921 tttcgactat accatcaaga atgtgacggt aagcaagaaa aaaatgaccc gggaaagcaa
     1981 attaataacg agagtaatct cataaccaaa tgcgataact cagacgctaa agttttgcca
     2041 cccggctacc gttatcagtt gcagagacaa taacgttaag gagttccctg tgagtcgagc
     2101 attgttcgcc gtagtattag catttccatt aattgcactg gctaacccgc attatcgtcc
     2161 ggacgtggag gtaaacgtac cgccagaagt gttcagttca ggcgggcagt cagcgcagcc
     2221 ctgtactcag tgctgcgttt atcaggatca aaattattct gaaggggcgg taattaaagc
     2281 cgaaggaata ctgctgcaat gccagcggga tgacaaaacc cttagcacta atccgctgct
     2341 ctggcggcgc gtaaagccat aaacgcctct aatagtggaa tgtcagcagg ggccagcgga
     2401 tattgcagcg cctc
//
LOCUS       DAJVRF010000266         2401 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJVRF010000266 DAJVRF010000000
VERSION     DAJVRF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.270"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(1..652)
                     /locus_tag="OQN13_004012"
                     /pseudo
     CDS             complement(1..652)
                     /locus_tag="OQN13_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(705..1301)
                     /locus_tag="OQN13_004013"
     CDS             complement(705..1301)
                     /locus_tag="OQN13_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1617211.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1328..1930)
                     /gene="yadL"
                     /locus_tag="OQN13_004014"
     CDS             complement(1328..1930)
                     /gene="yadL"
                     /locus_tag="OQN13_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HCS1617212.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(1945..>2401)
                     /locus_tag="OQN13_004015"
     CDS             complement(1945..>2401)
                     /locus_tag="OQN13_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1617213.1"
                     /translation="CNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLV
                     ASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKP
                     NSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          661 a          526 c          479 g          735 t
ORIGIN      
        1 gttgagttac tgttggatca aagtttgtat ctgtatagaa ttcaattgta agggtctgat
       61 aaataccacc tacagcccag tatttacggg tgctattcgc attattgcaa ctccaatata
      121 acacgtcttc gcttggatta taccacgaaa aatattgttc tgcagagtca ccaatgtata
      181 tcccaggtga actaacgtta gttacggtac cgtaagctga ccacatgttt gaaattaata
      241 atgtgtaata caaaccagga actgatgtat taaataattt atgggagcca tacattttac
      301 ctgagtcaac cattgcattt tcaatacgca ttgcgctacc acttgtgcta tcacttctgt
      361 tgcaataaag aaaaccacca ccagaagcgg tatctaactg actctactgt aattttacac
      421 ctgtaggtcc aagctgcgtc ggtttgtaga aaactaaatt atttgtactt gttgcatatc
      481 tgaatatgac agtagcatcg ccattcggcc ccgtttctct ccatttttga tctgacggac
      541 caacgccgta actattccca acaatatatc ctacgttatc atcagtgcca gcactcgcgg
      601 tataacaact caagcacaga attaaaaaaa aatgtctgaa gaatttcttc attatcactc
      661 caccatctac tgctgtaaca gtcgcagtta tttgttttga tctattattc gtagtacaca
      721 tcaactaata tatgactcat tacttggcca gatatgacat ccagcgcggg atcgattttt
      781 tgcattctgg cataaaattt ccatgaagaa ttatgcaggg cagaagtcat tactggatac
      841 acggaacgag aatttcctgc tgaactaata acattgaata tattggtcgg gtcctgaatg
      901 gaaaagagaa cgacaccgac atttttagcc ccggttgcga cttcattggc aaatatttgc
      961 cttgaacctg cagccatgac accgctaagg gctctaaaat caatatgtaa ctgattaagc
     1021 acatcgccag ttccctgtaa tgcacagtct gatactgtga cagtgaactc ttttccacct
     1081 ggttgatagc tttcaggagt aacattatct gaaaaatatc ccaccccaac gacgccaaga
     1141 tcaatattac cctggttact gattccgctt ttgcaagtgc tgttttttac attggcaaca
     1201 aggtcaatat cctgcccagc gatggctgga taggtaatgg gaaactgtag tgacagaaac
     1261 agaattattc tcttcatcag cttacgttga gtgggatgca tatatagttc ctggatctgt
     1321 cagtctgtta gttataggtt actgtaaatg tggcaggtgc agaaaattta cccgcagtga
     1381 cattgcttac ggttttattt ttttcgacga ccagtcgagc actcatcgga taataaacaa
     1441 ccgcgttggc tgcattggag atagtgacct cttgagatgc tggtgtcaca caactaaatt
     1501 gtgttgcgct ccctgttgcc ggagtttcgg tgctccagac ttcaacggcg acggcacttg
     1561 ctttatctgc ggctgtggat ccatttgcga gccccgcacc gtcattagca gtaccttcgc
     1621 atgtggctcg cttggttaat tttatttgtg cttttttatt ggggataccc gcacagtctt
     1681 tgaatttgag tttgaatgtt ttaactttgg tcttggcatt gatttcagaa atatatacat
     1741 cgccaaagtc aacaacagaa atgtcctgat cagagtcatt tactaatgta gcggtacaag
     1801 tacctatttc tacggtagta atcacggtca gcccaatgct gtctgttgcc gcataagagg
     1861 gtacgctgaa caatgaggca gccaaaacga cactgctgga taatccataa cgtaaatttt
     1921 taaatgtcat cttaattatc cttcttattg atagatgaaa ttaaacgttg ccagtgcacg
     1981 aaaattcccc ggtacgcctt gccctgcatc tgtttcacgt agcgcaacgg tcatttcaag
     2041 acccttatcg ggctgcatct cgtctgtgct ccagcgtatc ttttccgcac tgttaggttt
     2101 aaggaaagtg gcatcatcag tagtccgttt tttgaaaccc ataccgatat tacttgtcgt
     2161 cgaagatgaa tcaccagact gcggtataat aagcttaggt gagcttgatg atgcatttcc
     2221 ggtcagagtg gtatcaatcc aactgatgcc actgctgcac ccactagcaa ccagtttaaa
     2281 atcagcctga gattctgtcg ttttattcgc gatcttatcc agacccatct tagggattct
     2341 caatgtgtag ttattattgc catcattcgt gacgttatta ccagtaagtg tgatgttaca
     2401 g
//
LOCUS       DAJVRF010000267         2398 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJVRF010000267 DAJVRF010000000
VERSION     DAJVRF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.271"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..856)
                     /locus_tag="OQN13_004016"
     CDS             complement(<1..856)
                     /locus_tag="OQN13_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HCS1617214.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
     gene            complement(913..2091)
                     /gene="allB"
                     /locus_tag="OQN13_004017"
                     /pseudo
     CDS             complement(913..2091)
                     /gene="allB"
                     /locus_tag="OQN13_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            complement(2010..>2398)
                     /locus_tag="OQN13_004018"
     CDS             complement(2010..>2398)
                     /locus_tag="OQN13_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCS1617215.1"
                     /translation="TGTRQALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADK
                     IGHLQIADNPHRGEPGTGEINYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPY
                     FLNRVVATGKVMKPVLAQRQKVVSPP"
BASE COUNT          622 a          664 c          586 g          526 t
ORIGIN      
        1 atccggtcgc cacaaagctg cgacgataac gcgtattccc cgcgccctgc tgcggataaa
       61 aaacatccgt gccccgaatc gcaccgtagg tattgctgat atttaccaga cctgtaatca
      121 ccgccgtcag aataattccc ggcgacaaag caccgccact gccgagcggg aaccactgcc
      181 agtgcaactc accggagagc gaatgcgaag aaggaaagca aaagtaccac aacaaccagc
      241 cggttatggt gccgaccagc aggccataac gggcaaaacg ttgcggcaga aagataatca
      301 tcgccagtac caggcacatc accgccaccg agagcgcgaa cggtggtaac tgaattttaa
      361 aatatgggtc ggctatgcca aacggcagcc cgagcatacc tttgaaaaag atagtggtca
      421 gctgtgcgcc cagcatcaac ataaacaaga ccatcaccga cggcgtaaat aaccgtgcca
      481 ggcgatggcc taatccgcta aaaccaatca acatcgtcag cacgccggag agtgcaatcc
      541 ccaccgccag actggtagcg atatcgttga tcggtgtccc gcgcgatgct tctccaaggg
      601 tgatagtaag gatggttccc caccacaggc cacccggccc ttccataata gcgcgacgat
      661 gaccgcagaa cgcctgagcg aagcaggcca gtgcggtagc aagaaaagca tattgcgtga
      721 gcgtaagcag gctactttgc ggcaactgaa aagcagaaag tagcgtagga ggaaccacaa
      781 ccgtgttgca aaaaataaag aaaaaccact gaaatcctga taacaggctt tcgcggctga
      841 ctgcaaaatt gaacataaac acaccttaac cggagaatgc ccgccgcaag gacgggcatt
      901 gcaggggcca gattactgct gatgtttaag gataaattga cctttcggcg caacagggaa
      961 gccctgttca atgtcgtaaa tcacatcacc acgtaagatg gttttcgtga tacgcgcgcc
     1021 aatggtacgg ccaacatacg ggctgacttt gtggcgatat tccagatcgt cattggtaag
     1081 aacatagctg ctattcggct gaatgaagac gaagtcggca tcttttcctg gggcgatacg
     1141 gcctttttgc tgcagaccga aaatatctgc tgcgttagtc gccattaatt tgccgaacat
     1201 tggcagagac attccgcgtt tctgtaccgc ttcatcgaac atcacgtcca tacagttttg
     1261 cagaccggca ataccgcccc atgcttccat gatgttgccg gctttcattt ccggagggca
     1321 tggtgagtgg tcggaaacca ggcagtcgat ttcaccgtta aacagttttt cccacatgcc
     1381 tttctggttt tccagatcgc ggatcggcgg tgaacacttc gccagagtac cgatttcttc
     1441 gaactgatcg gtatccagta caaagtaatg cgggcaggat tcacaggtaa catcctgacc
     1501 ttcctgacgt gcacgagtca cttcttcaac actttccggg ctgctgatat ggcaaacgtg
     1561 cagacggcaa ccggcaactt tcgccagata cagcacgcgg cgaatcgctt ccacttcggt
     1621 aaataccgga cgcgaagcca catagtcatg ggcagttacg cgaccttcac gtttcgcttc
     1681 ttcgcccggt gcgtcacaga tcagcgcgtt ttcgcagtgc accaacaccg gctgccccag
     1741 ttcgcccagc ttctgcgcac ctttgaagaa ctgccagtcg tttacatcac ggaagtcgtt
     1801 gtcgataccg cgatcgccac aggtcgcaac gaagcatttg aagccgacaa cgcccacttc
     1861 atccagctca tgcagacggt cgatgttgta agacaccagg ccaccgagtt gtgccgcatc
     1921 gatagtcagc ttgcctttag cggcatcgaa cttcagttca attgaagcgc ggtcaaccgt
     1981 tgcaggcagc tggttgagcg gcatttcgat catggtggtg ataccacctt ttgccgctgc
     2041 gcgagtaccg gtttcataac cttcccagtg gctacgaccc ggttcagaaa gtacggatcc
     2101 atccagcgta aaccggcttc cgtggtggtt tggggtttat attcacaccc aacccaaccg
     2161 ttgtagtcag aattttcgat taccttaaag agataatcat aattaatttc tccggtcccc
     2221 ggttcgccgc gatgcggatt atcagcaatt tgcaggtgac caattttatc ggcccactga
     2281 gtcatggtgt tggttaattc accttccatc cgctgcatgt gataaatgtc atactgaatt
     2341 ttcaaattgc agcaaccaac atcatcaatc aatttcagcg cctgccgagt tccggtga
//
LOCUS       DAJVRF010000268         2395 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJVRF010000268 DAJVRF010000000
VERSION     DAJVRF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.272"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(77..153)
                     /locus_tag="OQN13_004019"
     tRNA            complement(77..153)
                     /locus_tag="OQN13_004019"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(117..119),aa:Arg,seq:tct)
     gene            complement(167..243)
                     /locus_tag="OQN13_004020"
     tRNA            complement(167..243)
                     /locus_tag="OQN13_004020"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(207..209),aa:Arg,seq:tcg)
     gene            complement(251..326)
                     /locus_tag="OQN13_004021"
     tRNA            complement(251..326)
                     /locus_tag="OQN13_004021"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(291..293),aa:Ile,seq:cat)
     gene            complement(367..1425)
                     /locus_tag="OQN13_004022"
     CDS             complement(367..1425)
                     /locus_tag="OQN13_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCS1617216.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(1533..1773)
                     /locus_tag="OQN13_004023"
                     /pseudo
     CDS             complement(1533..1773)
                     /locus_tag="OQN13_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1998..>2395)
                     /locus_tag="OQN13_004024"
     CDS             complement(1998..>2395)
                     /locus_tag="OQN13_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS1617217.1"
                     /translation="PVWAKCTKGVYPSYWEEWKKVREVVKVACPECGGKGEVSTACKD
                     CRGRGVAIHREESVKRGMPVIRDCQRCGGRGYERLPSTEAFNAICEVTNQITRASWEK
                     TVKKFYDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT          575 a          696 c          533 g          591 t
ORIGIN      
        1 cagacaactg tcaaccgtct gaattgtgag acatttaaaa aaaaggcccg caaaagcgag
       61 ccagggaaaa taagtgtggc gcgttgtact ggattcgaac cagtgaccga ttgcttagaa
      121 ggcaattgct ctgtccggct gagctaacaa cgcatgatgc agataatgga ccgccatcgg
      181 ggacttgaac cccgcgcagc cagcttcgaa ggctggcgct ctttcctgat gagctaatgg
      241 cggtatgtga tggtggccct tgctggattt gaaccagcga cctggcgatt atgagtcgct
      301 cgctctcacc actgagctaa agggccggga gccgcataat aacgacgcgt aattaattct
      361 tcaatatcat ccgttctggc tgactaaatc ctgtacttcc cgaaccgtct gctcaaaacg
      421 ttcagtctcc agctcaacgc caattgcacg acgcccgagc gccagtgccg ctttcactgt
      481 cgaacccaac cccatgaaaa aatctgcaat caggtcaccc ggacgactgc tagcgctgat
      541 tatctgctgc agcatttctg ccggtttttc gcacggatgt ttcccgggat agaactgcac
      601 cggtttatgc gtccacacat cggtatacgg cacctgcacc gtcacgccaa aataccgccg
      661 cagatgttta tattcactca gcagctccgc atactgccgg ttcagtgagg tatacgtatc
      721 caccagctgg tggtggagct tttccagttc accacgccga tgcttctctt ctgccacccg
      781 ggcaaacagc gcctgtaatt tcagataatc gctttcattc ggcaactgcc actgaccggc
      841 actgaaccag tgcgacacca tgtttttctt tcctgtggca tctgcaatct gttttgccgt
      901 tatccccagg gcagctcgcg catcacgaaa gtaagaaatc agcggggcca tcacatgctg
      961 tttcagtgca ctgcccttcg ccgcataccc ggcatctttc ggacgatacg gcccctgata
     1021 atgttccgcg aacagaatgc gctctgtggc ggggaaatac gcccgcaggc tttccttgtt
     1081 gcatccgttc cagcgtccgg acggcttcgc ccagataata tggttcagca cactgaagcg
     1141 ttcacgcatc atgatttcga tatcagatgc caggcgatga ccacagaaca ggtaaagact
     1201 tccggcaggt ttcagcaccc gccagaactg cgccagacac tggtccagcc acttcaggta
     1261 atcatcgtcg cccttccact ggttatccca gccctcaggc ttcactttaa agtacggcgg
     1321 gtccgtgact atcaggtcaa cagaattttc gggtaacgac cggataaatt ccaggcagtc
     1381 ggcgttgatt aactcacaac tggatatttt tacagtatta agcatggatc attaagcctg
     1441 tctctgatag gctcattctg cttttgcgca aagcagtggg cctgaggttt gcttgtgaac
     1501 ccaacgcatg agcagatggc tggtgggtgc ccctaacacc caccagccgc ccatttacca
     1561 caaataaaaa agccttcact gcggaaggcg tctgtaacaa ccgaactgat agtctgccag
     1621 acccgccata accagctggg tcagtattaa ctggcagcgt tcgcgtgaaa ggtaagtatt
     1681 ctgcgctatc tccccgactg tcgccggttc ggtaacgctt aattcattaa acaccactct
     1741 ggcggtttct gtcatatcct gctgttttag catgtctttt ccttttccgg ttaacgtgac
     1801 acaccaataa ctcttgtcga aaaagccagc aagctgaaag acaggtattc accgccacca
     1861 gcgcgtttac tgtactgacg cgatttcagt cataaaaaac ccgctcggcg gcgggttgta
     1921 gaaactcttc taacgtcagg cataaaacgc ccatcgttat gacgaattta ccacagattc
     1981 cggaaaaatc aaccttgtta cctagttacc ttttttaact gccgctcagc ccatgcttct
     2041 tcaatatcaa accgggtcac cagcgcatca taaaatttct taactgtttt ttcccatgac
     2101 gcgcgtgtta tctggtttgt cacctcgcat atagcattaa atgcctccgt tgatggtagt
     2161 ctttcatagc cacgaccacc acaacgctgg cagtctctga taacaggcat accacgtttt
     2221 accgactctt cacgatgaat ggcgacacca cgcccacggc aatccttaca ggcggtggaa
     2281 acctcaccct ttccgccaca ctccggacag gcaactttta ccacctccct gacttttttc
     2341 cattcttccc agtaagacgg atacacacct ttcgtacact ttgcccatac cggcg
//
LOCUS       DAJVRF010000269         2326 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJVRF010000269 DAJVRF010000000
VERSION     DAJVRF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.273"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..2151
                     /locus_tag="OQN13_004025"
     CDS             <1..2151
                     /locus_tag="OQN13_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1617218.1"
                     /translation="ALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQIALYRD
                     ARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPWRLRNYS
                     TWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLASDDNML
                     PNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSGDLEVTI
                     EESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEATAIYGLN
                     NTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGYQWRTQY
                     IKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTIFDGVSL
                     YASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSLNLSIPL
                     ERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHNSSLNAS
                     YRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDANGASGVR
                     IQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNRGAMVAA
                     RFNANIGYRVLVIVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISSKSQSWT
                     VRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            2162..>2326
                     /locus_tag="OQN13_004026"
     CDS             2162..>2326
                     /locus_tag="OQN13_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1617219.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          642 a          564 c          549 g          571 t
ORIGIN      
        1 gcgctggctg aaaaagatga cgatagcgtg atcaactcgc ttgaacaaat cattcccggt
       61 acagcagctg aatttgattt caatcatcag cgacttaatt tgagcattcc ccaaattgca
      121 ctgtaccgtg atgcaagagg ttacgtctcc ccttctcgtt gggacgatgg tataccaacg
      181 ctgtttacca actactcgtt tacaggttct gataaccgtt accgccaggg caatcgtagc
      241 caacgacagt acctgaatat gcaaaatggt gctaattttg gcccctggcg attacgcaac
      301 tattccacat ggacacgcaa cgatcagaca tcaagctgga ataccatcag tagttattta
      361 caacgtgata tcaaggcatt gaagtctcag ttgcttctgg gagaaagcgc caccagcggc
      421 agtatttttt ccagctacac ctttactggc gttcaactcg cttccgacga taatatgttg
      481 ccaaacagcc agcgcggatt tgccccaacg gtacgcggta tcgcaaacag tagtgcaatc
      541 gtgactatca ggcaaaatgg ttatgtgatc tatcaaagca acgtgccagc gggtgccttt
      601 gaaattaacg atctctaccc ctcttccaac agcggcgatt tagaagtcac gattgaagaa
      661 agtgacggta cgcaacgtcg ctttatccag ccttattctt cattacccat gatgcagcga
      721 cctgggcatc taaaatatag cgcgaccgct ggacgctatc gcgctgatgc aaacagtgat
      781 agcaaagaac ccgaatttgc tgaagccacg gcaatatatg gtttgaataa tacttttacg
      841 ctgtatggtg gcctgctcgg ttctgaagat tattatgcgc tggggatcgg tatcggcggc
      901 acacttggcg cactgggcgc gttgtcgatg gatatcaaca gagctgacac ccaattcgat
      961 aaccagcact cttttcatgg ctatcaatgg cgtacgcagt acatcaaaga tatcccggaa
     1021 accaacacca atatcgctgt cagctactat cgctatacca acgatggcta ttttagtttt
     1081 gatgaagcca atacccgtaa ttggaactat aacagtcgcc aaaaaagtga aattcaattc
     1141 aacatcagcc agacaatatt tgatggggta agtctgtatg cctccggttc gcagcaagac
     1201 tattggggca ataacgataa aaacaggaat atctcagttg gggtttccgg tcagcaatgg
     1261 ggaattggtt acagcctgaa ttatcaatac agtcgctaca cggatcaaaa taatgaccgc
     1321 gcactctctt tgaatctcag tattccgtta gaacgctggt taccgcgtag ccgggtttcc
     1381 tatcagatga ccagccagaa agatcgccca acccaacatg aaatgcgtct taatggctca
     1441 ctgctggatg atggtcgcct gagctatagc ctggaacaaa gtctggatga cgataacaac
     1501 cataacagta gcctgaacgc cagttaccgt tcaccttatg gcaccttcag tgccggatac
     1561 agctacggta atgacagcag ccaatacaat tacggcgtta ccggcggcgt ggttatccat
     1621 actcatggcg tgacgctctc gcaatatctg ggcaacgctt ttgcgcttat cgatgctaat
     1681 ggggcttctg gcgtgaggat acaaaactat ccggggattg ctactgatcc ctttggctat
     1741 gcagtggttc cttatctcac gacttaccag gaaaaccgtc tctcggtaga tactacgcag
     1801 ctgcccgata acgtcgatct tgagcaaaca acacagtttg tggtgcccaa cagaggtgca
     1861 atggtagcgg cgcgtttcaa cgccaatatc ggttatcgcg tacttgttat agtcagcgat
     1921 cgcaacggta aaccgttgcc ctttggcgct cttgccagca acgatgagac ggggcaacaa
     1981 agtatcgtcg atgagggcgg catactatat ctctctggga tatcgagtaa atcacaaagc
     2041 tggactgtac gctggggaaa tcaggcagat caacaatgtc agtttgcttt tagtacaccg
     2101 gattcagaac caacaacctc tgtattacaa ggcacggcgc agtgccatta aggataaaaa
     2161 aatgaaaaga gcgcctctta taacaggact tttgttgata tccacatcct gcgcttatgc
     2221 ctcctcagga gggtgtggag ctgacagcac tagcggtgcg acaaattaca gcagtgtggt
     2281 tgatgatgtt acggtgaacc agacagataa cgtgacagga cgggag
//
LOCUS       DAJVRF010000270         2236 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJVRF010000270 DAJVRF010000000
VERSION     DAJVRF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.274"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(1118..1453)
                     /locus_tag="OQN13_004027"
     CDS             complement(1118..1453)
                     /locus_tag="OQN13_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HCS1617220.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSVFFADTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
     gene            1582..1929
                     /locus_tag="OQN13_004028"
     CDS             1582..1929
                     /locus_tag="OQN13_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617221.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(1949..>2236)
                     /locus_tag="OQN13_004029"
     CDS             complement(1949..>2236)
                     /locus_tag="OQN13_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617222.1"
                     /translation="RAAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVV
                     RLDGSTCLQLWNKEGWVVRREGDPLEVAQWLPACHDAGMEVRVQINESAAP"
BASE COUNT          688 a          488 c          419 g          641 t
ORIGIN      
        1 tcacataaac caccgattga gtgacagtta ctgaaacaac tatttcgaca caaaatctcg
       61 tctattcaac atcatgcaaa tttgtcagtg actgaattta gtgacagaac cctctggaat
      121 tctgccgtgt aaccacttat atgcagacct gccccgttgc tctgggtttt caggctgcga
      181 tgagtgatga gcgatgattt ccatacagat gatatatcga ctggaattga ttgcctgcaa
      241 ttagatttag ttttattgtg tggatcgttt tagttagtgg ggatgaattt ccgataaatt
      301 aaccaatgga aaggagatga tatgaataaa aagataactg gattattaat aattagtctg
      361 gcgtcaattt tcttaattga ttgcacccag tgccattaac atctatctac acaaaaatag
      421 aggaaaacaa ttttgatata tcaaataatt acaagcagct ccattctttt tattgtttgg
      481 gggattacat ctataataat tagaacaaag aattattttt ctaagaagaa aacagacctt
      541 aatataagct tttttatagc cttccctatt tctataacaa tccttttatt ttgccgaggt
      601 atattataaa tataacataa tacatctata tggggtgtac atatccccac ataaaaacac
      661 atactctcat taacacggga agaaactatg ttttccaatt ctcactacat gtacataatc
      721 gttcttgtac tatggtttag tattctcaag tacttacaga gaaagaatag catattacat
      781 cctttatttt cactaatcct gacaccacta accattttaa tcattagact tatgatagat
      841 ttaacataca accaataaat tataaacaaa ataaaaatag ggaacattat atgctttata
      901 aaattttcac attaaacaca aagataaaaa caactatcat tacaatatgt gcattatcat
      961 ataatcttac agcgcaagct tccccgtcat acagacctcg tgatcgagtt aataagacta
     1021 tgatagaact acaaactaac ggagtagaac ggcaactttt agataattct tgtggcataa
     1081 gttctggggt atttaaataa aatataagac aggctgtcta tcttacagac agccttttta
     1141 taataacaga aagaatatat atcatactta acggaagaga taatatgaaa acagtacccg
     1201 tataaacagc aagcaatccc atttttccag gtgtatcagc aaaaaaaacg ctgttccaga
     1261 aatcaggccg ggtaaatttc agagctgtat cttcaatata ccattttgca accggataca
     1321 aaaccattcc gcagagagaa atacaccaga acagtaatct gtatttaaaa tcataatccc
     1381 atgacatata cagcatatat cctcccgtca cccatcccca ccacatatta ttaaaataat
     1441 attttctgtt catctgccag ttttcctttc ttttgcactt ttgtcagtgt actgatgcat
     1501 gacaacataa ccacagcatg ataataataa tcaataacaa taagctgtgt cacgtttaca
     1561 tcatggaacg gaggtgctcc tgtgactttt gagcagaaaa aagcccgggc cattgccctg
     1621 atggacagca aaaagatgtg gcggagcaat tatgcccctc ctcttctgcg tattctctgg
     1681 cggctgggta tccggcttcc gccattaccc tttatgccgt tctggcaggt cacggtgctg
     1741 acggggggct tatggggcat ttcctgggga tgtgcaatgt ggtttatcta ctgggggccg
     1801 tcaggaatgg tggcaggtga ggccattatt ataagtatca cggggggttt tttgtccgga
     1861 ctgctcatgg cctctttcca ctggtggcgc aggaaagtta accgactgcc accatgggat
     1921 gatgtgtaaa ccgtgcacag gcgtgttctt acggagcggc actttcattt atctgtacgc
     1981 gaacctccat tcctgcatcg tgacaggccg gcagccactg cgccacttcc agcggatcgc
     2041 cctcccggcg taccacccag ccttctttat tccataactg cagacaggtg ctgccgtcga
     2101 gacgcaccac aaaatcccca cggcaggcct gatacgttat gccgttcagc tccaccgcca
     2161 gatcagtggc agaagtgccg cctgcgggca gacctgctgc cgggaccagc ggcagtacga
     2221 cgacttctgc cgcacg
//
LOCUS       DAJVRF010000271         2231 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJVRF010000271 DAJVRF010000000
VERSION     DAJVRF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.275"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..264
                     /locus_tag="OQN13_004030"
     CDS             <1..264
                     /locus_tag="OQN13_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCS1617223.1"
                     /translation="AEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSG
                     IRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            434..712
                     /locus_tag="OQN13_004031"
     CDS             434..712
                     /locus_tag="OQN13_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCS1617224.1"
                     /translation="MGKPDVTDSNLKNIIDDLYRPNAKVGSGSTADAVRYELATGEKV
                     GGRGHVLKAQDYTRALQDWLDKNPTASPGDRAAAENVLQDLQNALGGK"
     gene            712..1089
                     /locus_tag="OQN13_004032"
     CDS             712..1089
                     /locus_tag="OQN13_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617225.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
     gene            1558..>2231
                     /locus_tag="OQN13_004033"
     CDS             1558..>2231
                     /locus_tag="OQN13_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCS1617226.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYA"
BASE COUNT          738 a          345 c          533 g          615 t
ORIGIN      
        1 gcagaaaagt tcgccacagc aatccgaaac cactggcacg tggagaataa gctgcactgg
       61 cgtctggacg tggtaatgaa tgaagacgac tgcaaaataa gaagaggaaa cgccgcagaa
      121 ttattttcag ggatacggca catcgctatt aatattttaa cgaatgataa ggtattcaag
      181 gcagggttaa gacgtaagat gcgaaaagca gccatggata gaaactatct cgcgtcagtc
      241 cttgcgggga gcgggctttc gtaatctttc cctagggagt cagccgaatg ttgggaagga
      301 tttgacggat gaagagaaga actgcttggc cggcggcagt acgggaagcc cgggaggatg
      361 gggaccagaa gatgagaata gcgcaaggga tcgtatgcag catgaacggt ataaagatga
      421 attgcgacag aaaatgggaa aacccgacgt tacagattca aatttgaaaa atattataga
      481 cgatctttat cggccaaatg caaaagtagg aagtggaagc acagcagatg ctgttcgtta
      541 tgaattggcc acaggtgaaa aagttggtgg tagaggacat gtattgaaag cacaggatta
      601 tacaagggct ttacaggatt ggttggataa gaatccaaca gcatctccag gggatcgagc
      661 agcagcagaa aacgttcttc aggatttaca gaatgctttg ggaggcaagt aatgaaccaa
      721 tatattgcta gttatataga aaaaatggca tcattttcca ctgttttgtc tgagacagtc
      781 tcattagcta atgaatactg gcatccagaa acgccaccgg tggttatgtt gttctctcag
      841 gttggaaaaa cgattgtaaa aaatttttat gaaatggggg atattgaaag agaaaaaata
      901 tttaaacatg tcgaagatgg tatggtctcg cttgatgatg aattggccac agttgtagct
      961 acagggctta tagaggcaat agtcactgcg accgatagca attctttact ttggcaagat
     1021 ttaagtaaat ttctgggtga caaatcaaag tctcatgcat tagtgtggaa aaattttggt
     1081 caggagtgat attattcaag aagtattaag ctcgctattg agatcttaat gcttcttgag
     1141 attaaattaa agttaggacg aagtaattgc aggtggaaat aaatccatag tgacgtgtgg
     1201 caatggctat tagatgttat ggtgtgatgg gctttggtga tttaagagct aagttagatt
     1261 gccttatgaa aaagtatggt tatatattta atgatgtgta tcatatattt aaataaaaaa
     1321 cggttctctc ttgtcaagat agtaatagga tagactattg tgttgataaa tataattaag
     1381 agtctacttt gttgatttta gaagactttg gagcagggaa agatcatcgg aagtacactt
     1441 tttgtactaa ataattcgcg ctaaatgttc aataattgag atattcctta ttacctaaaa
     1501 ctatttttca tagcttatat atgatcaaat actccttgca taaataagga gaacaaaatg
     1561 gaacttaaaa aactgatgga acatatttct attatccccg attacagaca agcctggaaa
     1621 gtagaacata aattgtcaga tattctactg ttgactattt gtgccgtcat ttctggtgca
     1681 gagggttggg aagatataga ggattttggt gaaacacatc tcgatttttt gaagcaatat
     1741 ggtgattttg aaaatggtat tcctgttcac gatactattg ccagagttgt atcctgtatc
     1801 agtcctgcaa aatttcacga gtgctttatt aactggatgc gtgactgcca ttcttcagat
     1861 gataaagacg tcattgcaat tgatggaaaa acgctccggc actcttatga caagagtcgc
     1921 cgcaagggag cgattcatgt cattagtgcg ttctcaacaa tgcacagtct ggttctcggg
     1981 cagattaaga cggatgagaa atccaatgag attacagcca ttcctgaact tcttaacatg
     2041 ctggatatta aaggaaaaat catcacaact gatgcgatgg gatgccagaa agatattgca
     2101 gagaagatac aaaaacaggg cggtgattat ttattcgcgg taaaaggaaa ccaggggcgg
     2161 cttaataaag cctttgagga aaaatttccg ctgaaagaat taaataatcc agagcatgac
     2221 agttacgcaa t
//
LOCUS       DAJVRF010000272         2181 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJVRF010000272 DAJVRF010000000
VERSION     DAJVRF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.276"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(49..651)
                     /locus_tag="OQN13_004034"
     CDS             complement(49..651)
                     /locus_tag="OQN13_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617227.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
     gene            893..1237
                     /locus_tag="OQN13_004035"
     CDS             893..1237
                     /locus_tag="OQN13_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617228.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGALH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            1360..1560
                     /gene="torI"
                     /locus_tag="OQN13_004036"
     CDS             1360..1560
                     /gene="torI"
                     /locus_tag="OQN13_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HCS1617229.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
BASE COUNT          672 a          492 c          441 g          576 t
ORIGIN      
        1 ttatattacc tcgataaatt aactaaaact tgcccgctat atactatctc attcagtatc
       61 atcacgcgcg gtctgtgcat atgtcactac cgcacctaat gtattaattt tcttttcaac
      121 atagataata ttatcgtact cataattgcc atacggatag caaatgcgaa tattctcatg
      181 tagatcgggg tcatccacct cagctccaga acaacttttt gaactaccgg aagtataccg
      241 atacggtgca acataagacg atgtctctcc aggcaaaaaa taagttagtg ttgtaagggg
      301 tataatcaga aaaaatccag aaaatatgca tatccctgca taaaccttaa ggtatgctga
      361 cagactcttc cagccgcttt gttttactat ccccttctta acccaaaaca gagataacag
      421 aaaagctatt cccatgctaa acagaatgta atagtgggat atactctgat taagaaacgt
      481 gaccctgtag atatctgccc gccaccagaa gaaaaggaaa ataaagatca gccctgaaac
      541 tgtcatgcaa atcaaataag gatacgaatc ttttttcatg tttagcgccc ataaaatttt
      601 tcctgccccg gacaaattta tcatccattt tttgcacaga aaatagctca ttacttactg
      661 cacaataata cacaaaattg cgtaaatttt ttgcatggat tttagctctt tcagccgacg
      721 tttaaggggt aaagagcatt tcctaaaagc aactgaccaa cccaacagaa tgggctaccg
      781 cttacgttga gagcaaaaaa gtgtatagca gcaatgaaca gcatcctcgc actgacgggg
      841 atttctttta tctgagctcg ctacggcgag ttttgtttta tggagacaag aaatgtcaga
      901 tttggctatg aaggttttga aatggcaatc aactggcgat gtcggcatca gtagcgcaac
      961 tcttgcctca atcgcatgtg gcctgaaaaa gaatatctat ggtcatcact tcggcgctct
     1021 ccatgacgca gcagactttc ggcgatgcgt tgcacttgtt gagcagattc cagaaatcag
     1081 agattcattc gacaaggttg caaagcgcgt tccggcattc aaaggaatcc tcaacgaatg
     1141 ggattcactc gttgctctgt tgaagtctga aatgaagacg tacgggaaca aagcaccaga
     1201 gacttacaga agaatcagcg agctacgcaa ggactaaccc gcctcacact cgatgaggcc
     1261 tgttcatttc tcaagatatc cagacctacc attgctgcat caatgcggct ttttattgcc
     1321 tgatttgcag gttcgattcc atattcggag atagcactca tgcaacacga actacaacct
     1381 gattcactgg ttgatttgaa attcatcatg gctgatactg gcttcggtaa gaccttcatc
     1441 tatgaccgga ttaagtccgg cgacctgcct aaagccaaag ttatccacgg acgagcaaga
     1501 tggttatatc gtgaccattg tgaattcaaa aataagctct taagccgcgc caatgggtaa
     1561 aatagcgggt aaaatatttt tcaaatctaa aaaacagcat tccaatcaat cccctgccgc
     1621 ctcaagtaga tgtctgcagg ggacaccaga tacccttcaa acgaaatcta ccttcacccc
     1681 gtaaaagatg ggtttggcag cacacttgcc ttatatctac tcatttttac tgcaacaggt
     1741 tgaaatctca gcactgtcag aaagcgctga tgactaaaca gccctgggcc gggcgatgta
     1801 accatcacac agaatcctga tagcgaaata tggcgtgact cgatacttca ctccgcaatg
     1861 cattccttga tgaattcgca ggaccgtgat acacgggaca ggtcgctgaa tgacgacaat
     1921 gtcctggaaa tcagcgaacc gcgcatctga agtacatttg agcgactgta ccagaacatg
     1981 aatgaggcgt ttggattagg tgattattag ccgggctaag cactttagta ttattatttt
     2041 ccggttgagg gatagggaga tatcgacaac aaccggaaaa gtttacgtct atattgctga
     2101 aggtacaggc gtttccataa ctatttgctc gcgtttttta ctcaggaaga aaatgccaaa
     2161 tagcaacatt aggcagacaa t
//
LOCUS       DAJVRF010000273         2171 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJVRF010000273 DAJVRF010000000
VERSION     DAJVRF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.277"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..1259
                     /locus_tag="OQN13_004037"
     CDS             <1..1259
                     /locus_tag="OQN13_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1617230.1"
                     /translation="EPQVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEVTWYG
                     WDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAKARHRSLAEVLQEDTGVTL
                     PAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAAQLEAEYIPERKLHLYHCD
                     HRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSLRLPGQQYDEESGLYYNRN
                     RYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGLKFHVNGDPSDFNQAVEYL
                     KQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMTIYWNSRASLFCSTELNSKSQ
                     SPALGLGHEFTHAQYCLLDKENFMALLSRTDKKYENKEEARVITIIESRAAKTLGECT
                     RGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            1271..1720
                     /locus_tag="OQN13_004038"
     CDS             1271..1720
                     /locus_tag="OQN13_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617231.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
     gene            1982..>2171
                     /locus_tag="OQN13_004039"
     CDS             1982..>2171
                     /locus_tag="OQN13_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCS1617232.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGD"
BASE COUNT          650 a          361 c          604 g          556 t
ORIGIN      
        1 gcgaaccaca ggtggagagc cggtacctct atgacccgct gggccgccgg acgggaaaac
       61 gggtatggcg gcgggagcgt gacctgacgg ggtggatgtc gctgtcgcgt aaaccggagg
      121 tgacatggta cgggtgggac ggtgacaggc tgacgacgat acagaccggc accacgcgta
      181 tccagacggt ataccagccg gggagcttca cgccgctcct gagaatcgaa acagagaatg
      241 gcgaacaggc gaaggcgcgg caccggagcc tggcggaggt gttgcaggag gacacgggtg
      301 tgacgctacc ggcggagctg tcggtgatgc tggggaggct ggagcgggaa ctgcgcgcgg
      361 gagcggtgag tgcggagagt gaggcatggc ttgcgcagtg cgggctgacg gcggagcaga
      421 tggccgcgca gctggaggcg gaatacatcc cggagaggaa acttcatctt taccactgcg
      481 accaccgggg actgccgctg gcgctcatca gcccggaagg ggaaacggcg tggcaggggg
      541 agtatgacga atggggaaac ctgctgggcg aaaccagcgc gcagcacctc cagcagtcac
      601 tccgtctgcc ggggcagcag tatgatgagg agtcggggct gtactacaac cgcaaccggt
      661 actatgaccc gttgcaggga aggtatatca cccaggatcc gatagggctg agaggtgaat
      721 ggaatctgta taaatatcca ttaaaccctg ttcggtttat agattctttg ggtttaaaat
      781 tccatgttaa cggtgatcca agtgatttta atcaggctgt tgagtattta aaacaagatt
      841 cacaaatgaa agaaactata gattttttat caagctctga agaaactata aatatagagt
      901 atattgaagg aaccaatgtt agatttaatt cgaataatat gacgatatat tggaatagta
      961 gagcatcttt attttgttca actgaattaa actcgaaatc acaatcgcct gcattaggtt
     1021 tgggacatga gtttactcat gcgcagtatt gccttcttga taaggaaaat ttcatggctc
     1081 ttttaagccg aacagataag aaatatgaga ataaagaaga ggcaagggtt attacaataa
     1141 ttgaatcgcg tgcggcgaaa actttaggtg agtgtaccag aggagctcat agtggactgc
     1201 ctttttatcg ggtggatgga ccgttgcaga cgatgaaaat aacgggtacg ccagaatagt
     1261 gaggtttttt atgatgaaat atttgatggt tttattgtct ctattttctg gttcggtatt
     1321 ggggatgggg cgagtgaatg aattgtgtgg aatagatagc gtgaaaacga tagagataat
     1381 taatcttcca tcgtatgtta ccacgttagt tccgcttagc aaggaaggtc ttaatgaaat
     1441 atacagatat aaagtggtgg tgaatgaaat tagagatctt tatgctggta agataattga
     1501 tttattgcaa atgaagtatt tcagaaaaga aaaatataac aatataagat gggggggtag
     1561 tatcatttcg aaaggtaata ataaatgtga aatttatttt gatgcttttg gtgaatgtgg
     1621 aagtgtgaat ggcattaacg tgtgttttga aaaaaatgaa atgataggtt ggattaaaaa
     1681 agaaatccct ttgctgtcgc aaaaaatagg tgggctttaa atggtagggc aatattcaaa
     1741 gacagtatag gtaactattt gtatggccat gtaaggtaaa agaaaaggaa taaaccatga
     1801 gcggaaaacc ggcggcgcgg cagggcgaca tgacccgcag ggaaagatca tcagaagttc
     1861 actttttgta ctaaataatt cgcattttat gtttaaaaat tgagatattc cttattacct
     1921 aaagctgttt tttattgctt atacatgatc aaatacttct tacataatta aggagaacaa
     1981 aatggaactt aaaaaattga tggaacatat ttctattatc cccgattaca gacaagcctg
     2041 gaaagtggaa cataaattat cggatattct actgttgact atttgtgccg ttatttctgg
     2101 tgcagagggt tgggaagata tagaggattt tggggaaaca catctcgatt ttttgaagca
     2161 atatggtgat t
//
LOCUS       DAJVRF010000274         2090 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJVRF010000274 DAJVRF010000000
VERSION     DAJVRF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.278"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..756)
                     /locus_tag="OQN13_004040"
     CDS             complement(<1..756)
                     /locus_tag="OQN13_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HCS1617233.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLIERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQ"
     gene            complement(913..1122)
                     /locus_tag="OQN13_004041"
     CDS             complement(913..1122)
                     /locus_tag="OQN13_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617234.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1145..1855)
                     /locus_tag="OQN13_004042"
     CDS             complement(1145..1855)
                     /locus_tag="OQN13_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1617235.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
BASE COUNT          593 a          423 c          351 g          723 t
ORIGIN      
        1 ctggtctaca ccactactat cagcaagatt cttaacaaat tctgtagctc gtttagcacc
       61 ttcatcacct aaaacgaatt gtgccgctcg ttgtgatgct tcttttttca gaccaacctc
      121 cataatccgc ctgtaagctt ctaaggctgt ctgaaaacta taaatggctg tacctgcttt
      181 gattgcactc gtagctaata catccagact atttgaagca ttttcactgt gattattttt
      241 gctggtggtc ttgttaaact tagaaagtgt ttcacgaaga gactgattta actttgctaa
      301 ctcacgatta aaagtttcgt ttccaagtgt cttggattta ctgcttaagc tgtaatactg
      361 gcttttaaaa tacttctcaa gcataggatt aaagtcacga agcttgctca tattcgctga
      421 gtaagctgct gtgattgctc tgcttccgct aattcgtggc tgtcttggtt gtctaagtgg
      481 ctttaaagta tcctcaattt cagttttaat cttaaacgct ctagtgtttt taagacgctg
      541 ctcaatcttg gaaacagttt tatcgacatt tctttctatt agtttgagag aatcatttaa
      601 aggcgtagga ttaactctaa acgacttatt caatcgcttc tcaattcgtg atgctacctg
      661 catcgtttgt ttctcggctt tttccaagcc ttttaaaact ttctgagagt taaaacttaa
      721 ctcaatcaga aaatcatcaa ctttaaattg tgccattcat atttttcacc tctttaagcg
      781 tgttgtacgt aaattctatt tttcagtgtg ggtattagct ttacagattt cacgtagcac
      841 acatacaaaa aaagggagag ctaccgatgt cactcgataa tctctccccg tatttaccca
      901 accaattctt ttttaatgtc ggacaagata atgtaattcc gtttcaccta tgatttgagc
      961 atcagaagga atacggtcaa tacaccactt tggaatcttg cgtgtttttg gtttgtgtga
     1021 ggtgataact tgacgtttgt ttttaattct taaggctggc ttagtttgta actttaagtt
     1081 ttcacaaaca gaccgaatag tctctaaacg ttcttcaaac attaatcacc tctttaattc
     1141 ataatcaaac agccttgcta tacttctgag aaatcttcgt taactgctca atatcagcca
     1201 tcaaagcaat cttcttctgc gactcatatt cactaacaag ttctttcata gtatcaagtt
     1261 cttttttaag cttttgcttt aacttatcca gtttaccgga ataatgctta gtcaaattag
     1321 cttcagctaa ctgaagaatc tcttcatctg ttttatcagt catcagaata aattgaccag
     1381 aaccccttaa tgcttgtctg acatcaatta accggaaccc ttcagggata ctttgaagaa
     1441 ctttaggaac gtcttcaatc gctgtaccaa aaccatcatt aaagtttcct tcataataga
     1501 taaacgggtt tgacttaatc tcgtaagcaa tatcatattc tgaaggtttc gaggtttctt
     1561 cgaagttctt aacagcatcg taagcgacat caagtctaag acgctgtgat tttaaagctg
     1621 ggtaatattc acgctcaagc tcaaatagtg cttcagtttt gcagatatca ggcatttctt
     1681 gaggctcagg agacacaaag gaaattacct ttggtgacat ctttagctct tgctcaagct
     1741 caatgcgctt gtcatgctct tgacgctgaa tagtatgtag cggatgttca ccctgtggtg
     1801 accagtctcc gaagctatca acctttttac cgttaatata taaatcgttt gtcattataa
     1861 tttcctctaa gtgtggttat ttcttaatag agcaccttca acgccaatac acttagaaga
     1921 tgacttaacg atttattaaa gatgctctat taagaaaagc cctaaacata atctgtgaag
     1981 ggctttggag gtttgcttat aacgttaacg aagtttcagg aaaactttct ttaaaagctc
     2041 tgtttaaaaa ctcttaaaga aagccctaac tcatgattag ggcaaagctg
//
LOCUS       DAJVRF010000275         2089 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJVRF010000275 DAJVRF010000000
VERSION     DAJVRF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.279"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(60..1025)
                     /locus_tag="OQN13_004043"
     CDS             complement(60..1025)
                     /locus_tag="OQN13_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCS1617236.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQIAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="OQN13_004044"
     CDS             complement(1879..>2089)
                     /locus_tag="OQN13_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617237.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          577 a          549 c          437 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcaatc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag acttgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcactccc gaacggctga gcccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAJVRF010000276         2065 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJVRF010000276 DAJVRF010000000
VERSION     DAJVRF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.280"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..1152
                     /gene="tyrR"
                     /locus_tag="OQN13_004045"
     CDS             <1..1152
                     /gene="tyrR"
                     /locus_tag="OQN13_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCS1617238.1"
                     /translation="GFNFLRWLESEPQDSHNEHVVINGQNFLMEITPVYLQDENDQHV
                     LTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVEQAQKLAMLSAPLLI
                     TGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELFGHAPEGKKGFFEQA
                     NGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVRVICVTQKNLVELVQ
                     KGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQGVQRPKLAADLNTV
                     LTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATVAVGEDAMEGSLDEI
                     TSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQKKNEE"
     gene            complement(1196..1702)
                     /gene="tpx"
                     /locus_tag="OQN13_004046"
     CDS             complement(1196..1702)
                     /gene="tpx"
                     /locus_tag="OQN13_004046"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCS1617239.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            1821..>2065
                     /locus_tag="OQN13_004047"
     CDS             1821..>2065
                     /locus_tag="OQN13_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309931.2"
                     /note="catalyzes the epimerization of L-alanyl-D-glutamate
                     to L-alanyl-L-glutamate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCS1617240.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKIL"
BASE COUNT          522 a          481 c          578 g          484 t
ORIGIN      
        1 ggctttaatt ttttacgttg gctggaaagc gaaccgcaag attcgcataa cgagcatgtc
       61 gttattaatg ggcagaattt cctgatggag attacgcctg tttatcttca ggatgaaaat
      121 gatcaacacg tcctgaccgg tgcggtggtg atgttgcgat caacgattcg tatgggccgc
      181 cagttgcaaa atgtcgccgc ccaggacgtc agcgccttca gtcaaattgt cgccgtcagc
      241 ccgaaaatga agcatgttgt cgaacaggcg cagaaactgg cgatgctaag cgcgccgctg
      301 ctgattacgg gtgacacagg tacaggtaaa gatctctttg cctacgcctg ccatcaggca
      361 agccccagag cgggcaaacc ttacctggcg ctgaactgtg cgtctatacc ggaagatgcg
      421 gtcgagagtg aactgtttgg tcatgctccg gaagggaaga aaggattctt tgagcaggcg
      481 aacggtggtt cggtgctgtt ggatgaaata ggggaaatgt caccacggat gcaggcgaaa
      541 ttactgcgtt tccttaatga tggcactttc cgtcgggttg gcgaagacca tgaggtgcat
      601 gtcgatgtgc gggtgatttg cgttacgcag aagaatctgg tcgaactggt gcaaaaaggc
      661 atgttccgtg aagatctcta ttatcgtctg aacgtgttga cgctcaatct gccgccgcta
      721 cgtgactgtc cgcaggacat catgccgtta actgagctgt tcgtcgcccg ctttgccgac
      781 gagcagggcg tgcagcgtcc gaaactggcc gctgacctga atactgtact tacgcgttat
      841 gcgtggccgg gaaatgtgcg gcagttaaag aacgctatct atcgcgcact gacacaactg
      901 gacggttatg agctgcgtcc acaggatatt ttgttgccgg attatgacgc cgcaacggta
      961 gccgtgggcg aagatgcgat ggaaggttcg ctggacgaaa tcaccagccg ttttgaacgc
     1021 tcggtattaa cccagcttta tcgcaattat cccagcacgc gcaaactggc aaaacgtctc
     1081 ggcgtttcac ataccgcgat tgccaataag ttgcgggaat atggtctgag tcagaagaag
     1141 aacgaagagt aagcgcgaat atgcctgatg gtgcaacacc atcaggcata ttaacttatg
     1201 ctttcagtac agccagagct gcttcgtaat ccggctcggt ggtgatttca tccaccagct
     1261 ggctgaaaat cacattgtca ttttcgtcaa taaccacaac ggcacgcgct gccagacctt
     1321 tcagtgggcc atcagcaatt gccacaccgt aagcttgcag aaattcagcg ttacggaaag
     1381 tggagagggt gataacgttg ttcagacctt ctgcgccgca gaaacgagac tgggcgaacg
     1441 gcagatcggc agagatacac agcacaacgg tgttgtcgat ctcagttgcc agttggttaa
     1501 acttgcgtac tgatgcggcg caaacatcgg tatcaatact cgggaaaatg ttcagcactt
     1561 tgcgtttacc cgcaaactga ccgagggtga cgtcagacag atcttttgcc acgagagtaa
     1621 aagtctgcgc tttgctaccc gcctgcggga tggaattggc gactgtaacc gggttgccct
     1681 ggaaatgaac ggtttgtgac atgattatct ttcctgttta catatagtta acgtcacacc
     1741 tagtttatgc taactgtcaa taacacagca aacgctattt gcgcttaatc cgcagaccac
     1801 cgcgacaaca aggagtaaag atgagaaccg ttaaggtatt tgaggaagcc tggcccttac
     1861 ataccccgtt tgtgattgcc cggggaagtc gcagtgaagc gcgcgtggtg gtggttgaac
     1921 tggaagaaga gggtattaaa ggcaccggcg aatgcacgcc gtatccgcgt tatggagaaa
     1981 gtgatgcctc ggtaatggcg caaattatga gcgtcgtgtc gcaattagag aaagggctga
     2041 cacgggagga gttgcaaaaa attct
//
LOCUS       DAJVRF010000277         2041 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJVRF010000277 DAJVRF010000000
VERSION     DAJVRF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.281"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..483
                     /locus_tag="OQN13_004048"
     CDS             <1..483
                     /locus_tag="OQN13_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCS1617241.1"
                     /translation="DYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPAPDES
                     VINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALMLRD
                     GVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDPARSK
                     "
     gene            483..1073
                     /locus_tag="OQN13_004049"
     CDS             483..1073
                     /locus_tag="OQN13_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCS1617242.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1060..>2041
                     /locus_tag="OQN13_004050"
     CDS             1060..>2041
                     /locus_tag="OQN13_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1617243.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRLATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEG
                     QGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGDN
                     DTGFKQNGDGLLDVYANSVHVLRFQSGSIQSNKAVNVTGRVTPSDYGNFDARYQQRNG
                     GVQDVRYGYEMYYTPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGV"
BASE COUNT          511 a          446 c          600 g          484 t
ORIGIN      
        1 gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac tttcagacac
       61 tggaaaggca ctggaacggt tggcgtgatg gtggcgacaa acgatccgga acgcccggcc
      121 ccggatgaaa gcgtgattaa cgcagtcagg gaccacatcc ttcctctggc acctgttgcc
      181 ggaagcggat tgtatgtatt cggtgccaca gaaaaagtca tcccgatgac gattgcatta
      241 tcgaaagaca caccgcagat caggactgca ataaaagcag agctgaatgc actgatgtta
      301 cgggatggtg tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc catcagtttg
      361 tctgctggtg aagtggccca ccggttaatc gtcccttcat cggatattga cctgggggag
      421 accgagcttc cggtgctcgg cgagatcacc tggcaggctt atgacccggc aaggagtaaa
      481 taatggattc gttacaggat gattatacaa agctgctgta cggactgatg ccgccggggc
      541 ctgcatggtc agataccgac ggtgtacttg acggtctggc accatcgctt gtgcgtgttc
      601 accagcgggc tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca gagctgattg
      661 aacgttatga agaattgtat ggtttacctg attcctgttc ccctgtcgga acccagacat
      721 tacgccagcg tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc ataaatgagc
      781 agttcttcct ggatcagctt gaggcgctgg gatataccgg cgtaacgatc gaacagttcc
      841 agcacttgga tgcaagcccc gatccggaat ggggagatcg ctggcgttat ttctggcgtg
      901 tgacgttgcc gatggatgcc ggtgctcagt ggcagacatg cacggacgcc tgcaacacac
      961 cgatccggac gtggggggat acggttgcgg agtgcgtgat taataaatta tgtccgtcac
     1021 acaccgtcgt tttattttcc tatccagatg aggatgaaga tgcacaggat tgatacgctg
     1081 actgcggtaa aagataagtt tggtccgggt aagaacggat ttactgacgg aaatcttcgc
     1141 acaggacgtc ttgctacctg gctgaacagt gccatgtgga atgccattca ggaggaaatc
     1201 tgtggtgtca ttgagaaggc cgggatagaa ctgaataaag aagaacacga ccagctatat
     1261 aaggctatat tattgctggt gggcggtgca attaacgaag aggcattgct gataaaaaat
     1321 aacctttcgg atgtggaaga cagggatgag gctgttgaaa acctcggatt aaaacccacg
     1381 gtggataagg caaaaaatgc cgttcagcgt gatggcgaca ccatgaccgg agagctgaaa
     1441 atccgtggtg ttaatgcgct gaggattttc aacgaagctt ttggcctgat ttttcgtcgt
     1501 tcggaagagt gcctgcacct tattcccacc agtgaaggtc agggggaaaa tggcgatatt
     1561 ggtcccctgc gcccgttcac cattaatctg cggacgggtg aaatatccat gtcgcataaa
     1621 gtgtctgttg gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt tcagaacgcc
     1681 ctggggggga attcaattgt tcttggtgat aatgacaccg gattcaaaca gaatggtgat
     1741 ggcctgctgg atgtttatgc caatagcgtg catgtgttgc gttttcagag tggcagtatc
     1801 cagagtaata aagctgtaaa cgttacagga cgggtaacac cgtcagacta cggaaacttt
     1861 gatgcccgtt accagcagcg aaatggtggc gtgcaggatg tgcggtatgg ttatgaaatg
     1921 tattacaccc ccggcagtaa caccgtttcg tggacatttc gctcaccttc gggacacggg
     1981 ctgtcaggga tatcgatatc ggataccggc cgtaactcag cggataacgt caacggcgtg
     2041 t
//
LOCUS       DAJVRF010000278         1977 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJVRF010000278 DAJVRF010000000
VERSION     DAJVRF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.282"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..599)
                     /locus_tag="OQN13_004051"
     CDS             complement(<1..599)
                     /locus_tag="OQN13_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1617244.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVA"
     gene            692..1075
                     /gene="crcB"
                     /locus_tag="OQN13_004052"
     CDS             692..1075
                     /gene="crcB"
                     /locus_tag="OQN13_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCS1617245.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1129..1338)
                     /gene="cspE"
                     /locus_tag="OQN13_004053"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1129..1338)
                     /gene="cspE"
                     /locus_tag="OQN13_004053"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS1617246.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(1513..>1977)
                     /gene="pagP"
                     /locus_tag="OQN13_004054"
     CDS             complement(1513..>1977)
                     /gene="pagP"
                     /locus_tag="OQN13_004054"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCS1617247.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          504 a          493 c          453 g          527 t
ORIGIN      
        1 gccaccatat aacaggtggt atccagcgca cgagcggcaa gcaacgttga ccagtgatgc
       61 tctttgagcg gcccgcgaac ccaggcggca ggaagtacca ggatttcagc tccctgtaat
      121 gcctgtgcca gcgccagctc tggaaagcgt aagtcataac aggtcatcag accgaccttc
      181 atcccctcca cctcgagtaa cggagcgatt tcattaccag catcaacacg gcgtgattcc
      241 tgaatggcaa atgcatcata gagatgcaat ttggcataac gggcgacgat gtttcctgcc
      301 tgtagcgcca ccagcatatt ccatgcgcgc cccggcgttg aaggaacatg aatcgtcaga
      361 attgtcgtca tcatgttacg tttactttct cgccgtaaac gtccgaggaa ttcgccttcc
      421 agcagctgtg ctgatttaac cgatagatct gcatcatgat catcgcgcgc cagcaatgct
      481 tccggcagga cgaacagcga tacgtcgttt tccgccgcct gcgccatcaa cgaggcacaa
      541 atctcagcgt tcttttccca cacagatgta acagcaaact gtcctgcggc aaccagcatt
      601 gccacctcct gtcagagtcg taaaattcgc caacgacgat tattcggcgt tacacttatc
      661 actcatacaa atcaaatagc aggattttgc agtgttacaa cttcttttag cagtttttat
      721 tggcggtggt acgggaagcg tggcgagatg gctgttaagt atgcgattta acccgctgca
      781 tcaggcgatt ccgttgggga cgctggcagc aaacctgatt ggggcattca tcataggaat
      841 gggattcgcc tggttcagca ggatgacgaa cattgatcca gtgtggaaag tattaatcac
      901 caccggattt tgtggcggtc taacaacctt ctcaacattt tcggcagaag tggtgttttt
      961 gttacaagag ggccgctttg gctgggcgtt actgaacgtt ttcgtcaacc ttctggggtc
     1021 ttttgccatg accgcactgg ctttctggct gttttcggcc tcaaccgcac actaaagacc
     1081 aaaaaaaacc cgctgattaa gcgggttttg aattcttgct gacgtatctt acagagcgat
     1141 tacgtttgca gcagaagggc ctttggcacc gttagtgatt tcgaactcta cgcgctgacc
     1201 ttcagcaaga gttttaaaac cattagtctg tattgcagag aagtgtacga acacgtcttt
     1261 gctgccgtct tccggagtaa tgaaaccgaa tcctttggac tcattaaacc acttaacgtt
     1321 acctttaatc ttagacatca aaattacctt tacatgaaaa atagacacaa atgctgtgtc
     1381 ggttaccagt acaccaattg tggtacgctt tgtccagtgc aactttacta aaaagtgata
     1441 aaagtcgcgt tatttttgat gttacttatt ttagttattg attttaaaga agttactaaa
     1501 acttcatttg tctcaaaact gaaagcgcat ccaggcaaag tacacattgc cattgttgta
     1561 ggtacccgga atgtaggtca tctgaaaagt cactgggcca taacccacgg aggccaatgg
     1621 cagtagaacc gggagaggga tgtaattcca gttatcgcgt gccgttacgc cagcggtgaa
     1681 tcccagacct aaatgaaaat tttcatccgc cagcggtcgc caggtacttt cccatccgta
     1741 tccggcaatc ggttcccatt tgttccacga gtccttaaat gccatggcat acaggccatg
     1801 ccagtttcct ttttcatccc atcgcgactg accaaaaccg ccaccccacg gtcgctcgtt
     1861 atagcgatcg gttttttctt tgtcgtaagc gaaacgtgca tgccaggtga tggcaggaat
     1921 atataaatca taatgttcag gctgttgcca ggtttgtgca atattttctc taaacgt
//
LOCUS       DAJVRF010000279         1887 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJVRF010000279 DAJVRF010000000
VERSION     DAJVRF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.283"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..389
                     /locus_tag="OQN13_004055"
     CDS             <1..389
                     /locus_tag="OQN13_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617248.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="OQN13_004056"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="OQN13_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCS1617249.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="OQN13_004057"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="OQN13_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCS1617250.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="OQN13_004058"
     tRNA            complement(1806..1881)
                     /locus_tag="OQN13_004058"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
BASE COUNT          503 a          446 c          457 g          481 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattag
//
LOCUS       DAJVRF010000280         1862 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJVRF010000280 DAJVRF010000000
VERSION     DAJVRF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.284"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            163..498
                     /gene="iraM"
                     /locus_tag="OQN13_004059"
     CDS             163..498
                     /gene="iraM"
                     /locus_tag="OQN13_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCS1617251.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(779..910)
                     /locus_tag="OQN13_004060"
     CDS             complement(779..910)
                     /locus_tag="OQN13_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCS1617252.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1410..1486)
                     /locus_tag="OQN13_004061"
     tRNA            complement(1410..1486)
                     /locus_tag="OQN13_004061"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1450..1452),aa:Arg,seq:tct)
     gene            complement(1587..1662)
                     /locus_tag="OQN13_004062"
     tRNA            complement(1587..1662)
                     /locus_tag="OQN13_004062"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1627..1629),aa:Ile,seq:cat)
     gene            complement(1765..>1862)
                     /locus_tag="OQN13_004063"
     CDS             complement(1765..>1862)
                     /locus_tag="OQN13_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS1617253.1"
                     /translation="VTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          601 a          463 c          332 g          466 t
ORIGIN      
        1 gcagatatgc atggataaac tacaagacga gatataaacc accctgcatt taaataaaca
       61 ataaacaaca tcagaaaaat aattctgctc tatggtttac attcaaaaat atcatttata
      121 cttttcagaa catcaccagc aaggcataaa caaggaaact aaatgaagtg gattgtgatt
      181 gatacagtta tccagccatc atgcggaata tctttttcag tcatatggag taaaataaaa
      241 ttaataatct ggtatcaatc ggatgctttc ttacctcctg aaagtatatt tacactgact
      301 cacacaggca tcatgctcaa taacaaagtg ctacctgtaa ccatttacaa cgtagtacca
      361 ttcaataaaa cattctggaa ttcaatcaaa aatagccagg aatgccctac aaatacagat
      421 aacgtattga atgaatgctt taataaccgt tgcactctgc aaatatgtcc ttatggacta
      481 aaacaacaaa gtccataagg agtttactca catctgacaa aatcaatata aacagcccct
      541 ccggagaggg gctggagagt ggcgctatgt gccattgcat ggtgccgggt gcctcccggt
      601 gaattcagta ccagcacctg aatccgcgat tatcccatat acctactcgc tgattgcccc
      661 tccgcacagg gggattcacc atgccagttt cttttaacaa actccccgca aaccagacaa
      721 cagtcaaccg cctgaattgt gaagtattta aaaatttctc ccgctaactg atacccggct
      781 aacagtctgg cgttttcttt ttcagcaacg ggaaagcaac aaccaccaca cccgccacca
      841 gcacaccgtc agccagcact gacattatcc ggctgctgca atgccattca caaaaacagt
      901 aagcaatcac tttttaccgt aacaggtgat aatccagata tgtatctacc ccagatgagt
      961 aatccgaagt tcatccatac cacaggtcct ggctattctg ttgtactcct gaacaagagc
     1021 aaataattct gaattagcaa ccatgaactc atcgcaaacc ctctgtatag catcactatt
     1081 cagaataata acgtctcttc ccgaaagacg atcaggagta cagaacaaaa ctgtcaaacg
     1141 gctgaaggcc tttgctcgtg ctgcattgac tatatcaata cgctgcctaa ggatgaaaca
     1201 ccccgacgcc tcatcaatat tcactctacc cacaccatat gaatgataaa tatttaatgc
     1261 tgaaaaaacc attagaccgt ataacaaaca ctcaatcaat acttaacaga acttttattt
     1321 ttgacaaaca taaaatattt tcaacaatat cctgagccag gtatatttca gtataaggct
     1381 ctgccggaag gaatctggaa gaatgaatat ggcgcgctgt actggattcg aaccagtgac
     1441 cgattgctta gaaggcaatt gctctgtccg gctgagctaa caacgctgaa taccgataat
     1501 ggaccgccat cggggacccg cccccgcacc aacaaccctg ttatcgtgtc gtctgctctt
     1561 cctgataagc taatggcggt ttgtgatggt ggcccttgct ggatttgaac cagcgacttg
     1621 gcgattatga gtcgctcgct ctcaccactg agctaaaggg ccgggagcag aataataacg
     1681 gtccgtaatt aattccgcaa taaaaaaccc gctcggcggc gggttgtaga aactcttcta
     1741 acgtcaggca taaaacgccc atagttatga cgaatttacc acagattccg gaaaaatcaa
     1801 cctagttacc ttttttaact gccgctcagc ccatgcttct tcaatatcaa accgagtcac
     1861 ca
//
LOCUS       DAJVRF010000281         1841 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJVRF010000281 DAJVRF010000000
VERSION     DAJVRF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.285"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..403
                     /locus_tag="OQN13_004064"
     CDS             <1..403
                     /locus_tag="OQN13_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1617254.1"
                     /translation="WQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLDWFYEVRHLST
                     SLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKDAVLSPAAERF
                     FALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(444..1439)
                     /gene="pgl"
                     /locus_tag="OQN13_004065"
     CDS             complement(444..1439)
                     /gene="pgl"
                     /locus_tag="OQN13_004065"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCS1617255.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIVAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1699..1830)
                     /locus_tag="OQN13_004066"
     CDS             complement(1699..1830)
                     /locus_tag="OQN13_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCS1617256.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVERMGSWPEVVMA"
BASE COUNT          476 a          495 c          431 g          439 t
ORIGIN      
        1 atggcaggag ttagtgggtt acaaaatgat tggcgtacgc tcctccagcg gcaaccgact
       61 gttgatagaa caacagctgg ccgacaagcc ctggaagctg gactggtttt acgaagtgcg
      121 tcatctttcg acgtcattag gactggtaga ggcgggactg gggatttcgg cgcttcctgg
      181 tctggcaatg ccccatgcgc cctattcttc gattattggt attccactgg tggaacccgt
      241 tatacgtcgg acattgggga ttattcgccg caaagatgcc gtgctttctc cggcagcaga
      301 acgctttttt gccttgctga ttaatttgtg gactgacgat aaagacaatt tgtggaccaa
      361 tatcgtcgaa cgccagagac atgcgttaca ggagataggt taatacaccg gcgcaggaga
      421 gactgcgccg ggtaaatcag cggttagtgt gcgttaacca ccacccacat tggtccctgc
      481 ccgaccgcat agcggccttt ttcatgcagt agcccctgct cgccaacaat ttcgtatacc
      541 gagatgtggt gagatttttg cccggcgaca atcagatact tgccgctgtg atcaacattg
      601 aagccgcgcg gctgggtttc cgttggctgg aagccttctt tactcaacac gctgccatct
      661 tccgaaacgc tgaaaacggt aatcagactg gcggtacggt cgcaggcgta taaatgacga
      721 ccatccgggg tgatatgaat atcagccgcc caacaggtgt cggagaagtt ttccggcatc
      781 atatccagcg tctggacaca ttcaatattg ccgtgtggat ctttcagttc ccagacatcc
      841 actgagctgt ttaactcatt gacgcaatac gcatattgtt cgtttggatg gaataccata
      901 tgacgcgggc cggccccttc aacggtagtc acttccgcag ggtcctgcgc caccagatga
      961 ccatcatcgc tgaccgtaaa caggcaaatg cgatcctgct ttaatgccgg aacccacagc
     1021 gtacggttgt ccggtgagat attggcggaa tggcaaccgt ccagcccctc gaccacatcg
     1081 acgacgccca ctggcaggcc atcttccaga cgcgttacgc tcacgttacc cgcattgtaa
     1141 gaacctacaa agacaaactg cccctggtga tcggtggaaa tatgcgtcgg actacccggc
     1201 agcgcagact ctgcggcaaa ggtcagtgcg ccatcgtccg gggcaatacg atacgccagg
     1261 acgcgaaact cagggcgaac accaacatag agataacgtt tgtccgggct gaccaccatc
     1321 ggctgcacct gccccggcac atcgacaacc tgtgtcagcg tcagtgcgcc ttcatgattc
     1381 aggttccaga cgtgaatttg ctggctctca gggctggcga tataaactgt ttgcttcatg
     1441 aacacacctc aaaaatgata aatcccttat ctttcataca cataacaaca cttactggat
     1501 aaaaaacaga caacaaaaca cgataaaata caatatcgca catcaattaa atcaataagt
     1561 tagcactcca tttcgaatgg catattacta aaatattagt caccgacacg aatttgacaa
     1621 tgccacagat attttcgcga ctattcaggg agtatgactc tccccgcttt tgtttacctg
     1681 tatcaacgac cgtaaacact atgccataac aacttctggc caacttccca ttcgctcaac
     1741 aatcaactca cttccggcac aaataaacgc attacgttgt agatcggtat tctggtcatt
     1801 tgttgatacg cgtacatagc caataagcat ggtaaatccc c
//
LOCUS       DAJVRF010000282         1835 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJVRF010000282 DAJVRF010000000
VERSION     DAJVRF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.286"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..413
                     /locus_tag="OQN13_004067"
     CDS             <1..413
                     /locus_tag="OQN13_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCS1617257.1"
                     /translation="RRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLD
                     NLEATGLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTD
                     KWQRRTHSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            350..982
                     /locus_tag="OQN13_004068"
     CDS             350..982
                     /locus_tag="OQN13_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCS1617258.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRAMATQLPAFRQKLSD
                     GWYQVRIAGQDVSTSGLTAQLHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVMPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            1043..>1835
                     /locus_tag="OQN13_004069"
     CDS             1043..>1835
                     /locus_tag="OQN13_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCS1617259.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTL"
BASE COUNT          403 a          468 c          575 g          389 t
ORIGIN      
        1 ggcggcgctt tgagcacggg gtgacggact gttacacgct gttccgggat gcttaccatc
       61 tggcggggat tgagatgccg gattttcatc gtgaggatga ctggtggcgt cacggtcaga
      121 atctctatct ggataatctg gaggccacag ggctgtatca ggtgccgttg tcagcggcgc
      181 agccgggcga tgtgctgctg tgctgttttg gttcatcggt gccgaatcat gccgccattt
      241 actgtggtga cggcgagctg ctgcaccata ttcctgaaca actgagcaaa cgagagaggt
      301 ataccgacaa atggcagcga cgcacacact ccctctggcg tcaccgggca tggcacgcat
      361 ctgcctttac ggggatttgc aacgatttgg ccgccgcatc gaccttcgtg tgaaaacggg
      421 tgccgaagcc atccgggcga tggccacaca gctccctgcg tttcgtcaga agctgagcga
      481 cggctggtat caggtgcgta ttgccgggca ggatgttagc acgtccggat taacggcaca
      541 gttacatgag gctctgcctg acggcgctgt gattcatatt gttcccagag tcgccggggc
      601 caagtcaggt ggtgtattcc agattgtcct gggagcagcc gccattgccg gatcattctt
      661 taccgccgga gccacccttg cagcatgggg ggctgccatt ggtgccggtg gtatgaccgg
      721 cattctgttt tctctcggtg ccagtatggt gctcggcggt gtggctcaga tgctggcacc
      781 gaaagccaga actccccgca cacagacaac ggataacggt aagcagaaca cctatttctc
      841 ctcactggat aacatggttg cccagggcaa tgttatgcct gttctgtatg gtgaaatgcg
      901 cgtggggtca cgtgtggtat ctcaggagat cagcacggca gacgaagggg acggtggtca
      961 ggttgtggtg attggtcgct gatgcaaaat gttttatgtg aaatcgcctc cgggcggttt
     1021 tatcgtttat ggagcatgac gaatgggtaa aggcagcagt aaggggcata ccccgcgcga
     1081 agcgaaggac aacctgaagt ccacgcagct gctgagtgtg atcgatgcca tcagcgaagg
     1141 gccggttgaa ggtccggtgg atggattaaa aagcgtgctg ctgaacagta cgccggtgct
     1201 ggacagtgag gggaatacca acatctccgg cgtcacggtg gtgttccggg caggtgagca
     1261 ggagcagtca ccgccggagg gatttgaatc ctccggctcc gagacggtgc tgggtacgga
     1321 agtgaaatat gacacgccga tcacccggac catcacgtcg gcaaacattg accgactgcg
     1381 ttttaccttc ggtgtacagg cactggtgga aaccacctca aagggagaca ggaatccgtc
     1441 tgaagttcgc ctgctggttc agatacagcg taacggtggc tgggtgacgg aaaaagacat
     1501 caccattaag ggcaaaacca cctcgcagta tctggcctcg gtggtggtgg ataacctgcc
     1561 gccgcgcccg tttaatatcc ggatgcgcag aatgacgccg gacagcacca cagaccagct
     1621 gcagaacaaa acgctctggt cgtcatacac cgaaatcatc gatgtgaaac agtgctaccc
     1681 gaacacggca ctggtcggcg tacaggtgga ttcggagcag ttcggcagcc agcaggtgag
     1741 ccgtaattat catcttcgcg ggcgcattct gcaggtgccg tcgaactata atccgcagac
     1801 gcggcaatac agcggtatct gggacggaac gttaa
//
LOCUS       DAJVRF010000283         1827 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJVRF010000283 DAJVRF010000000
VERSION     DAJVRF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.287"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(359..478)
                     /locus_tag="OQN13_004070"
     CDS             complement(359..478)
                     /locus_tag="OQN13_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCS1617260.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(439..624)
                     /locus_tag="OQN13_004071"
     CDS             complement(439..624)
                     /locus_tag="OQN13_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617261.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            725..898
                     /locus_tag="OQN13_004072"
     CDS             725..898
                     /locus_tag="OQN13_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617262.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            900..1244
                     /locus_tag="OQN13_004073"
     CDS             900..1244
                     /locus_tag="OQN13_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCS1617263.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            1248..1583
                     /locus_tag="OQN13_004074"
     CDS             1248..1583
                     /locus_tag="OQN13_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCS1617264.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
BASE COUNT          521 a          404 c          411 g          491 t
ORIGIN      
        1 ttccacctcc ccctttacac cttaaggctg ttcccacgca cagaacataa tgtttatgcc
       61 tggctgagta aataacctta ttgttagtgt ggatacactg ggggatgata accccgcaat
      121 ttcattattc attgttctcc cataggatga ggtttcccgc cgtaactggc gggctttttt
      181 tgccccagaa ttttcccttt tagccccctg taaacgaaaa ctgcgccgaa gcgcagttta
      241 atcgttgatg gtcagaacga ccagttcacg atatatactg cgggtatcga ctgatttcga
      301 ttccatgcca ttggataaat gatagtagcg tctaagtgac ctaagctctg ctgcaacatc
      361 agcccgcagg caccagacca ggggattcat cctgaagaga cagcgcaggt gtattgtgtt
      421 caccgctcac caaagacatc atgatgaaat gatgatatcc cgcataagaa agaggcattt
      481 tttaaacgca gtgcgctgaa gtgtagttgg ataaaaaatc actcttttca gataatactg
      541 tcgtacgctt atctttcgac aatgaggccg tcggcaaaat aaaatgacta atgtaatcgt
      601 tgctgatgaa tatcttctgc tcacataaaa atcacacaat aatattgata aatcagatta
      661 tcttaccttt acaacattgc gttaacagtc aatgatactt cttgaataaa aatattgcca
      721 atcaatgagt tattcctcat ttaagattat tctcattcac gtaaaaaaca taattcctat
      781 catcacagcc acgctcattt tgaattatct gaataatagc gagcgttctt tagttaagca
      841 gatcttaatt gaagacgaaa ttattgtgtg tgcaacttac ttaataccgg acatctaagt
      901 tgaagaaaaa aatacttgcc ttcgggctta tctcagcatt gttctgctcc acgccagcga
      961 tggcggatat gaaccgcacc acgaaaggtg cattgctagg cgcaggcgtt ggattactta
     1021 ctggcaatgg tgttaatggc gtactgaaag gtgcagctgt tggcgctggt gttggtgcag
     1081 taacagaaaa aggccgcgac ggtaaaaatg cacgtaaagg tgcaaaagtg ggtgccgccg
     1141 ttggtgctgt cactggcgtt ctgaccggca atggcctcga aggggcgatt aaaggtgccg
     1201 tgattggcgg tacgggtggt gcaattctgg gtaaaatgaa ataaggaatg atgatgaaaa
     1261 aatttgcttt actggcgggc ctgtttgttt tcgcaccaat gacctgggcg caggactaca
     1321 atattaaaaa cggtttgccg tcagaaacct atattacttg cgcagaagcc aacgaaatgg
     1381 caaaaactga cagcgcacag gttgcagaaa ttgttgcggt gatgggtaat gccagcgttg
     1441 ccagccgtga tttaaaaatt gagcaatctc cggaactgag cgccaaagtg gtagaaaaac
     1501 tgaatcaggt ttgcgctaaa gacccgcaaa tgctgttgat cacggcaatt gatgacacca
     1561 tgcgtgctat cggcaaaaaa taatatttaa tatgttaccg cgtcgatgaa ttcgacgcgg
     1621 tattttagct aacggggatc agaacttata tttcaggccc accatcactg cggtgtcatt
     1681 gtagccatta tcacccagct gcacgcccac attaccccag acactggccg ccggatggat
     1741 ttgcccctat atttccagac acctgttatc acttaaccca ttactggctt gctgccgtag
     1801 atattcccgt ggcgagcgat aacccag
//
LOCUS       DAJVRF010000284         1750 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJVRF010000284 DAJVRF010000000
VERSION     DAJVRF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.288"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(50..193)
                     /locus_tag="OQN13_004075"
                     /pseudo
     CDS             complement(50..193)
                     /locus_tag="OQN13_004075"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(197..1018)
                     /locus_tag="OQN13_004076"
     CDS             complement(197..1018)
                     /locus_tag="OQN13_004076"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS1617265.1"
                     /translation="MFSHPVYLFLREFSLQDSRGGSLSKKAESLSSGKITIGTKEKVI
                     IINELRQCHPLSQLLVIADLPRSTFYYHVKRLNAPDPYQLVKQVILRIYHQHKGRYGY
                     RRIRLACRNESILLNGKTIRKLMKELGISSLIRRKKYRTYRGEQGRTCNNLLKRQFYA
                     DRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEIVSYNLTERPLASMVKSMLLDAVEQ
                     LNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQSMSRRGNCLDNAAMESFFSTLK"
     gene            1007..1750
                     /locus_tag="OQN13_004077"
                     /pseudo
     CDS             1007..1750
                     /locus_tag="OQN13_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    1287..1403
                     /locus_tag="OQN13_004077"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          446 a          389 c          413 g          502 t
ORIGIN      
        1 cgtaggtgcc caccttttca aatgatctgc gccccaaatc ttggacatcc tatgaaaaat
       61 gtttaagtcg atattgaacc ggactcatgc catcgagttt ctctttaatg cgatggtgat
      121 tgtagtattc aatgtaatca attatatctt gctttagatg atgtaactta ttgtatttat
      181 ttaggtagta acattcttat tttaaagtac taaaaaagct ttccattgct gcattatcta
      241 agcagttacc acgtcgggac atgctttgtg ttatgccatt atcactcaac caacgttgcc
      301 aacgaggcat ctgatattgc cacccctgat cactatgcaa caagggctta tcgtctttat
      361 tgagttgttc taccgcatct aatagcatgc tctttaccat tgatgctaat gggcgctcag
      421 taaggttata tgagactatt tctccattat acaggtccat gattggtgat aaatagagct
      481 tcctgccatc gaccttaaac tctgtgacat ctgttaccca cttttgattt gggcggtcag
      541 cataaaattg tctcttcagt aaattattac aagttcgccc ctgttctcca cgataggtac
      601 ggtacttctt gcgcctgata agtgagctga tacccagttc cttcattaat tttctgatgg
      661 ttttaccatt aagtaaaatg ctctcgttac ggcaggcaag tctgatgcgt cgataaccat
      721 aacgcccttt atgctgatgg tatattctca atattacttg tttaacaagt tgatatggat
      781 ctggcgcatt aaggcgtttc acgtgatagt aaaatgtact acgtggcaaa tccgcgataa
      841 ccagtaattg tgataatgga tgacattgcc ttaactcatt aataatgatt actttttctt
      901 ttgttccaat cgtaattttt cctgagctaa ggctttcagc ttttttagat aatgaaccgc
      961 cccgggaatc ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac
     1021 tcgttttccc ccgaagtccg tcaacgggca gtccgtatgg ttctggaaag tcagggcgaa
     1081 tatgactcac aatgggcggt aatttgttcc attactccaa agattggctg tacaccggag
     1141 actctgcgtg tctgggttcg ccagcatgag cgggatacca ggggcggtga tggagggctc
     1201 accaccgctg aacgtcagcg tctgaaagag ctggagcgtg agaatcgtga actgcgctgc
     1261 agtaacgata tccttcgcca ggcttccgct tattttgcga aggcggagtt cgaccgcctc
     1321 tggaaaaaat aatgccactg ctggataagc tgcgtgggca gtacggggtc ggaccggtat
     1381 gcagtgaact gcatattgcc ccgtcaacgt attaccactg tcagcaacag gacatcatcc
     1441 tgataaacgc agtgcccgtg cgcagcacga tgactggctg aagagagaga tacagcgcgt
     1501 atacgatgaa aatcatcagg tgtacggtgt gcgtaaagtc tggcgccagt tgttacggga
     1561 aggaatcagg gtggccagat gtacagtggc gcgcctcatg gcggttatgg gacttgtcgg
     1621 tgttctccgg ggtaaaaagg tccgcactac cgtcagccgg aaaaccgttg ccgcaggtga
     1681 ccgcgtaaac cgtcagttcg tggcagaacg tcctgaccag ctgtgggtgg ctgattttac
     1741 ttacgtcagc
//
LOCUS       DAJVRF010000285         1749 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJVRF010000285 DAJVRF010000000
VERSION     DAJVRF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.289"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..946)
                     /locus_tag="OQN13_004078"
     CDS             complement(<1..946)
                     /locus_tag="OQN13_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCS1617266.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGV"
     gene            complement(992..>1749)
                     /locus_tag="OQN13_004079"
     CDS             complement(992..>1749)
                     /locus_tag="OQN13_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617267.1"
                     /translation="DALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKSL
                     QQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAVI
                     MYCLSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMFL
                     AQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTKI
                     LEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
BASE COUNT          440 a          491 c          439 g          379 t
ORIGIN      
        1 tcacacctaa caaccacggt ctggtggtgc gcggatctaa acgcatcacc tctgggtaaa
       61 aacttttacc cagcaaaccc gctggcgttc cggcggcgtc aaagaatgtc atggcgattt
      121 taaataaccc ggcggcagcg ggtcctaaca cgatccccac cagcactgtg ctgcacgagt
      181 tacgcgccga ccagatggag tgggcaatgt tggttgacca gacaaaactc cacgcgcctt
      241 tgatgtgtcg ggcagactca aacagattca atttgaaggc attatggata tttctgcggc
      301 gtaattcgcg cgctgcaaac caccagtaca tggtgccgcc aaccagattc gacacgtacc
      361 aggcaataac aaaacccgca aaaccaaagt caaaatacca ggctacgacg ctccccgctg
      421 cgcgcagaaa aggtttcgtc gcctgctgta cagcaattaa atcgaagcga tctaccgcac
      481 gcagaatgcc ggtcggcgtg gaggaagcca ttgaaggaat gagcgtgcaa tacagcgccg
      541 ccagccaaaa gctttggtca tccagaccta atgaatggga aaggaatggc agtaaggcaa
      601 tgccgccgac aatcgccacc gcgccgctga cgatatccag cgagaaggaa aatgagacga
      661 cattgcgaaa ttgctgcgga ttattgttgg tcaaggctgg cgttccgtac tgaaccacca
      721 gctgccatgt ctgaaactta ataaaatcgc tgatcgactt ggcgtacgat tgcacaatca
      781 ccagtacgcc aaacatggcg ggcgtcatcc ctttaccggc acacgagagc gccaacagcc
      841 ccagcaaggc gctcacgaca ttactggagc ctaaccaggc gctattgcga ataatggtgc
      901 gaaacgcgcc atctgcaaac caatgtttga tgttaaaacc cgccaattca gcctgacctt
      961 atcgttgata gtaaaaagta tcccgccagc cttaagttaa acttcggcgg tcagaaacga
     1021 tggcaaccag agaaaccgcc ttctgtgcct gttccagcac ttcgctgtag ggcgctctgg
     1081 aatcaatctc aagaattttt gtgccgttat agccaatctt cgacatgaca ccgattttgt
     1141 cctgcagctc ggcatagtca tggtcaggct tgcgggagat ggcagtctca atatcaatgc
     1201 ccaggcgaat aattaattcc gggcgatatt gcgccatttg ttggtataaa cgccgttcgc
     1261 gctgcgccag aaacatgctg attttcccgg tcgcacgttc gacgccaatc cccggtccgt
     1321 cgtaataaaa gcccgaaatt tcagcctgcg ggaagcgatc gctgaccacc agaacgccac
     1381 tttgcgccag tcgctgaacc ttgcgtagat tcgccattct tcgcagcgat aagcagtaca
     1441 taatcaccgc cgcccatagc gccggagatt tggttttcat gctttgggtt ttcgaggatt
     1501 tggccgccag tcgccgttcc agccagacgc caaccaacgg taatcgtttg attttgtcgc
     1561 cgtcttcgcc cgagagcagc cccagatagc gccgctcggt ttgccagtgc tgttgcaatg
     1621 atttcaccag atcggtggtc agcgtggatt taccggtgcc atcgcacccg accaccgcaa
     1681 ttagccccgg aatgtagctg ggctgcggcg cggttgtact attaacagtt tgtgtttgta
     1741 gtgcatcca
//
LOCUS       DAJVRF010000286         1736 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJVRF010000286 DAJVRF010000000
VERSION     DAJVRF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.290"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(94..1581)
                     /gene="yeeO"
                     /locus_tag="OQN13_004080"
     CDS             complement(94..1581)
                     /gene="yeeO"
                     /locus_tag="OQN13_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCS1617268.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
BASE COUNT          546 a          474 c          358 g          358 t
ORIGIN      
        1 gaacgaggcg aatattagcg atgcccaccc acaatgtcaa agcctgtttt ttaaatttga
       61 aatcgtttgc tgaaataatc tgcattttgt cgtttattcc gacacaactg gctttttttc
      121 acacttttgc ggttcggatc gagggtattt ccacagccaa cgtccagtaa ccattcgcca
      181 gtaaaacagc acggcccgca cagcccagtc ggcaaacatt cccatccaga caccaaccac
      241 accccagcca agcatgatgc ccagcacata accgacgaca acccggcaac cccacatgct
      301 caacatagaa acccacatgg cgtaacgggc atcgcgagca cctttaaatc cagcgggtag
      361 cacccatgag gcggaccaaa taggcataaa taaagcattt agccaaatca gaatcacaac
      421 gacatgttta acctgtggat cctgggtgta aaacgatgcc ataaccccgg caaagggagc
      481 cgttagccag gcgatggccg ttaatccaag agtggaaagc cagaacacat gccgcaactg
      541 aatctctgct tgcgctatct gccctacccc caaccttcgg cctgtaatga tcgtagaagc
      601 agagccgagc gcacttccgg gtaagttgat aagagccgca attgaaaacg cgataaaatt
      661 tccggcaata acactggtcc ccatcccggc aacgaacatt tgggttaata accgaccact
      721 ggtaaataac actgattcga cactcgcggg aataccaatc cccatgactt cccagataat
      781 gctaaaattc agcggtttaa aatagctctt taacgaaatc cttagcgcag gattaaaacc
      841 aatcgccagc acccacaaaa ttgcaactgc gccaatataa cgagaaatgg ttaaacccag
      901 ccctgccccg acaaatccca gtcccggcca ggagaaaagg ccgtaaatca atatgccgct
      961 aataataata ttaagaatat tcaggctacc gttaatcaat agcggtattt tcgtattccc
     1021 tgcaccacga agtgccccgc taccaataag agtgatggca gctgctggat aactgagtac
     1081 cgtcagctcc agataagtca acgccagtgc tttaacttct gtcgtggcat cacccgcgac
     1141 gaaatcaata atttgttcgc caaaatgatg aataagcgtt gccaacagta cggcaaacaa
     1201 cgtcatgatc accaatgact gccgcgtcgc caccctcgct cgtcgtcgat cccgcttacc
     1261 gagactaaat gccacaacga cagtagtacc aagatcgata gcagcaaaaa aagccataat
     1321 gaccatattg aagctgtccg ccaatcccac gccggccatc gcatcttttc ccagccagct
     1381 gaccagaaaa gtgctcagaa cccccatcaa caggacacag gcattctcca tgaagatagg
     1441 aacagcaagc ggggttatct cgcgccagaa caacactttg tagctcttgc gtttagcgtg
     1501 ccagcgagtg ccgtgaacaa cctggcgtaa agcagaggag atattcaaag ccgaccttaa
     1561 ttgcagaaag tgaaaccaca tttcaaataa tgagggagaa tcagcaaagc tgcaaagatt
     1621 ttcgccaaca aattgtctgc aaatgcaaca aactgttgat agaaacggca aacagttggg
     1681 gaatttaaaa atcgggtttg acaaaagatt tttcgccgtt aagatgtgcc tcaaca
//
LOCUS       DAJVRF010000287         1615 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJVRF010000287 DAJVRF010000000
VERSION     DAJVRF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.291"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..239)
                     /locus_tag="OQN13_004081"
     CDS             complement(<1..239)
                     /locus_tag="OQN13_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HCS1617269.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEA"
     gene            complement(244..942)
                     /locus_tag="OQN13_004082"
     CDS             complement(244..942)
                     /locus_tag="OQN13_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCS1617270.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            complement(952..1281)
                     /locus_tag="OQN13_004083"
     CDS             complement(952..1281)
                     /locus_tag="OQN13_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1617271.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVKIRVRKGEHPFLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            complement(1281..>1615)
                     /locus_tag="OQN13_004084"
     CDS             complement(1281..>1615)
                     /locus_tag="OQN13_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1617272.1"
                     /translation="DLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKL
                     GGVADTGGSGMVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDG
                     GRLSGAYR"
BASE COUNT          346 a          539 c          367 g          363 t
ORIGIN      
        1 gcctcactca gccagggcag accaccggga tgactgtgga ccagcgccac aatctcaccc
       61 tgcatttgtg cccgcagcca gtcctccgga gccatccgga accgttcctc cggctcaccg
      121 gagatattct cgctgggaag atacctgtct ccctccggcg ttcttaccac gaagccacac
      181 gactccgctg gcgcacatcg ccgggcgtgc gccagaatcg ctgattctgt ctgtgtcatg
      241 gatttactga gaaagtttat tgatggaaag gaaaccgccg aaattaccgg tattgttgcg
      301 caactcacac ccgcgcatac acttgctgca tttgtccttc cggatatccg tcgttggttt
      361 gtcgaactcg tcagccactg ccctgcccgt gtaaccacac tcatcagaac ggtaagtcca
      421 catacaggta ttcgccagca tgatacgccc cgggaaaacc gcgccatccg tctccgttgg
      481 tgtggccagc acaaaagagg cactgaccgc gctcagttcg ctgcactgct cgatgcgcca
      541 gcggctgatc acctcctgtt ccggatcggc gtcgctgttt ccgttgacga agttcaccgc
      601 atccagaaaa cgggcgtaaa ccttacgcct gaccaccgtt ccgccgacca gactctgcag
      661 gtcttccgcc atcccggtga ccattccgtg caggttagaa accgtcagtg tcggacgggc
      721 agcactgccc ttgccgttca gttcaaatcc cgtcccctga atggggtatg cctgatactg
      781 ccgcccctgc caggtaaccg gctcaccttt ttcgttctgc tcattacaga aaaaataacg
      841 ttcaccaccg acctctgtca gatcgatttc ccagagcacc acctgggctg actgagtgag
      901 gcgtgtcgtc tcatgatgtg tttcctgtgg aatatcctgc atcagagcct cctatgccac
      961 gacctgttca aaatctgccg ttatggttac ccacaacgcc cccacgcttg ccgaccattt
     1021 acgacaaacc accctgatcg gcttccagtc ataaggtggc gtccactgaa atgcgcggac
     1081 gccaccgtgc cgttccagaa aggcttttaa aaatgggtgt tcacctttac gaacacgtat
     1141 cttcacgctg taagtcgaca actggttatt cagtcccgcc gcacgacgct gttcataacc
     1201 atcgcccagc ttcactgtca ccactttcgg ctctgatacc acattcatat ccgggcgcac
     1261 tttccagtga aacgtctcca ttaccggtat gctccactta accggccacc atcacgggcc
     1321 tgctgttgca taaagtccgc ggccgctttt ttcccgaggt cataaaccac cttcagggca
     1381 gccggaccta tctgcccgtt cgtgccatcg ttattgatct caatgttgta ctgcggggca
     1441 aacatcacca tgcctgaacc accagtatcc gccacacccc ccagcttacc atcagcaccg
     1501 cgacgcagtg gcagaatggc ttcaggtccc gcttccccca tcacacccgc gccttttgca
     1561 aaagcaaaaa acgtcggacg gttaaccacc gtgccactgt agcgactcaa atcag
//
LOCUS       DAJVRF010000288         1579 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJVRF010000288 DAJVRF010000000
VERSION     DAJVRF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.292"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..436)
                     /locus_tag="OQN13_004085"
     CDS             complement(<1..436)
                     /locus_tag="OQN13_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1617273.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFTDEAVSGSVK
                     ASLRNGFSRLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGR"
     gene            1235..1531
                     /locus_tag="OQN13_004086"
     CDS             1235..1531
                     /locus_tag="OQN13_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCS1617274.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          453 a          342 c          313 g          471 t
ORIGIN      
        1 gcctccccat atgtacccct tcagctttag cacgttcaat accagccttt ctacgctctg
       61 ctatcaagct tctttcaagc tgtgccaacg ttgacataat gcctaacatt gcttcaccca
      121 tagcgcttga taagtcaaac ccttctctaa ggctgataac tgtcacacct ttagcctgta
      181 gcgctttaac cgttgataac acatcaattg tgtcacgtcc aagacggtct acagctacca
      241 caactacggt gtcaccttct cgtgcataag ctaatagtct gctgaaaccg tttcttaaag
      301 aagctttgac agaaccactg acagcttcat cagtgaacca tttagcaatg ttgtaccctg
      361 cctcttcaat ttgcatttgc tggttagtga tggattggtc atctgtactc accctgcaat
      421 atccaagtat cgccataatg ttccccttag aagtctcaaa agttagtctc ataagtttag
      481 ctgtctcata atccaaaaac aaccttttga gactacttga gggtgcattc taccgttacg
      541 ttgaaagtgt ctcaaaagga agtcctttta agactggtta tgaaggagct atgaggttga
      601 ttaggggttg gtacaagccc aactaaccga atcaactatg aagattttag gagggtaact
      661 gtaaccatca ctttctaact ttcacgtagg aaattgttaa gtggtttgat agttacacca
      721 tgtgaccaag catctatata tcgttatata gccctgtata tagcactata tgtagtgtcg
      781 agagaggaac gactaacacg atacagttta ccctgaaaag agacttcaca gattctttat
      841 atatttcaat aagttagggt aaaaagggac tataccgaga ctgtatagaa tctgtgtaga
      901 attgcataga tacttggaaa tctactatta aattgcatag ggactcacct gatattgtcc
      961 tgtttttaac aggtctccta gaaaaccgaa tagacttcat tcaagggtca ttgaagtcat
     1021 aatcatttca gtagaaaaac caagtctcga ttttaaatgc aaatagcaat catattcaat
     1081 aaggcaagac gatcaggatc agtgaaaatg tcatttttgg cgtaattgac cttactttat
     1141 gcatggtctt aacaaagaca tcatctttcc gttgcagatc ttcgcccttt caaataacgt
     1201 actttacaac tttcccgaac aaggagttgt gcccgtgttg tatgttattt acgctcaaga
     1261 caaagctgat agcctcgaaa aacgcctttc cgttcgtccg gcacatttag cacgtttaca
     1321 gttactgcat gatgaaggtc gtttgttgac agctggtcca atgccagcgg tagacagtaa
     1381 cgatccaggg gctgcgggat ttactggctc aaccgttatt gctgaatttg aatcgctgga
     1441 agcagctcag gcctgggccg atgctgaccc gtacgtagcg gcaggtgtct atgaacacgt
     1501 ttcagtgaaa ccatttaaga aagtgttctg aatagagatt caatatttaa gggcacggtt
     1561 tttgcaaccg tgttcttgt
//
LOCUS       DAJVRF010000289         1501 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJVRF010000289 DAJVRF010000000
VERSION     DAJVRF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.293"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..657)
                     /locus_tag="OQN13_004087"
     CDS             complement(<1..657)
                     /locus_tag="OQN13_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCS1617275.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     L"
     gene            complement(929..>1501)
                     /locus_tag="OQN13_004088"
     CDS             complement(929..>1501)
                     /locus_tag="OQN13_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HCS1617276.1"
                     /translation="DCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELNDTVVDIAK
                     QVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFKRLGDTLRY
                     LQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVKEIRWMIEE
                     LRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT          368 a          396 c          345 g          392 t
ORIGIN      
        1 taaacgcggc agctcgccag tgagtagtga gagataacgc tcaaccggcc ataatccttg
       61 taactgatta ataaagatta cactgtctgc gttatttcct aactgaggaa cgaatccggg
      121 ataacttaac cagcgtggtg catcgggatt gtccccagtc atacggcgga acgtcgccat
      181 cgtgcgccgc tgcatggtgg ggtcctgaac aacgatagcc gtatgaactc gttctacggc
      241 ctgattcaat agcgcgatgc taaagcgtgc gttttcaccg cagtttgttg actggtcttc
      301 aatccagatt ttttcatgcg gaatgtgcca gaactgatga gcgatatccg cctggatggt
      361 cgcttctgct ctgccagtgg tgcggatagt gttgtagtgc ggatgctgtg cgatggcgct
      421 atacaaaaaa gttgtcgagt gaccgatacc accactaatc agtaaaggaa tttgctgatc
      481 gcgagcaatc ttacatgccg cgtcgatagt cggcataacc gcattgcctg caaggatcac
      541 gcaatcggcc tgatacggca cctcaccgga gaaatcatcc tgcgccagcc actgtccgat
      601 aacatttatg gcatctatag ttgccggcga aagcgtcgga aacggggtaa tgttcatggt
      661 tcctccttat cgttccctta agaatactgc gtgagataat agtgagaagc ggagcactct
      721 gaaaggagga agtcgtattt ccttaaccgc cactccgcaa gcctgacata tcttttaagg
      781 aaatgctgta gataaacatt attcatcttg cagcagagaa ttgtattttt ctgaattcct
      841 gaatttttac ccttaccgtt ttagaattaa taaaaaaccc ggcgctaaat gcttaccggg
      901 ttttttcttt atcaggcaaa tagcagggtt aaccgctaat ctgctccatc gcctgcaaaa
      961 tacgcttatc tgaaatcgga taaggcgtac caagttgttg agcgaagtaa ctaacacgca
     1021 actcttctat catccaacgg atctctttca cgtcttcatc ctcacgacgt gcaggcggca
     1081 gtttgttgat ccattgctgc cacgcctgct ggacgttttc gactttcagc atctgagcac
     1141 ggtcgcgatg tggatcaacc gccagttttt ccagccgttt ttcaatcgcc tgcaaatatc
     1201 gcagcgtgtc gcccagccgt ttgaagccgt taccagtgac aaaaccgcga tataccagcc
     1261 cgcccatctg cgctttaatg tcagaaagcc ccagcgccat cgtcatatcc acccgcccct
     1321 tcagacgttt gttgatattg aacactgccg taaggatttg ctcgacctgc ttcgcaatat
     1381 ccaccaccgt gtcgttcagt tcggcacgca ctttttcatg cagcgcagca aagccttctt
     1441 ccgtccagac cgggccacca ttggcgtcga tcaatttatc cacaccgcag gagatacagt
     1501 c
//
LOCUS       DAJVRF010000290         1453 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJVRF010000290 DAJVRF010000000
VERSION     DAJVRF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.294"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..1010)
                     /gene="cadA"
                     /locus_tag="OQN13_004089"
     CDS             complement(<1..1010)
                     /gene="cadA"
                     /locus_tag="OQN13_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HCS1617277.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHAAIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1090..1437)
                     /locus_tag="OQN13_004090"
     CDS             complement(1090..1437)
                     /locus_tag="OQN13_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HCS1617278.1"
                     /translation="MTALIILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDL
                     IRFEGVNIRNFVSLICSVLGCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQ
                     SHSMDNHTASNAH"
BASE COUNT          404 a          319 c          363 g          367 t
ORIGIN      
        1 gtgtaaggca cccacgcgga gtcaaagtgg atggatttca catccagtgt tttcttgatg
       61 aagtcggtgt tgtacagcag accatcatag gtagagttgg taattacagc atgtaccggc
      121 caggttgcgt ttggtgtttc tttcacgcgc ttagcaatgg cagcgtgctg gaattcactc
      181 tgtgggatac caccaagaat accgtaagcg ttacgggtcg ggcggaaata gattggcgta
      241 acatcgctca tcatcatcag gtgggtcagc gatttgtggc agttacggtc aatcagaatg
      301 gtgctgcctg ccggagcaga gtacatgcca acaattttgt tcgcagtgga agtaccgttg
      361 gtcaccatgt agctgcggtc tgcgttaaag acgcgagcga tatactgttc tgcttctttg
      421 tgtggaccac tatgatccag cagagaaccc agttcagata ctgaaatgga aatatcagat
      481 ttcatggtat tcggaccaaa gaaatcatag aacaggctac ctaccgggct tttctggaat
      541 gcagtaccgc ccatgtgacc aggagtacag aaagtatatt taccttcacg aacatattta
      601 aacagtgctt tagtcagcgg aggcagaata gtgttgatat attcgtcagt ggtctgcttg
      661 atcttattag caatatcttc agcagcaccc agcgcatatt caaagaagct aatctgtaaa
      721 cgcaggtcat tcaggcttac atcgagagtg gaatacgtat tagcgaacgc gtacaacggc
      781 aggttctcgt tcattttgct aatttcttcg cacagctcga gattatattt atcccagtca
      841 aaaataacgc cgcacagacg cgcattgttt tcgatcagtt ttaataagtc gtcacggtcg
      901 ttcgggtaaa caatctggaa gttcagacgt tcaagcgcgc gatgaagttc acggatgggt
      961 tcttctttaa aataaacccc catgtgattc aatattgcaa taacgttcat agtcatatct
     1021 ccaggtaaaa aaggcccctc ccaacacatg ggacaaaatg aaaggaggag cctcggaaaa
     1081 tacttttaat taatgtgcgt tagacgcggt gtggttatcc attgagtggc tctggcgctc
     1141 gtgcattttg cgagcgtaga acatcaggat aatcaggctg acgatgaagg tacctgccag
     1201 ctcgaaggag cttgcgccca tcagcgcgat gaagcagaac acacaaccca gtacagagca
     1261 gatcaggctg acaaagttgc ggatgttaac gccttcaaaa cgaatcaggt caacgcaaga
     1321 gtagaagtac ggcagcatag tcagcagtac tgcgataccg gtcagttcac cgaacaggtc
     1381 agatgcttta ccaccggcag agttcatcag agtgatgagg attatcaggg cagtcatttt
     1441 cactgcagcc agc
//
LOCUS       DAJVRF010000291         1421 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJVRF010000291 DAJVRF010000000
VERSION     DAJVRF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.295"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            16..429
                     /locus_tag="OQN13_004091"
     CDS             16..429
                     /locus_tag="OQN13_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS1617279.1"
                     /translation="MHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIK
                     LFWRPANIRLIPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPF
                     ETLTLPLPANSAGGQMTWRFINDYGAVSDPIKMTL"
     gene            454..>1421
                     /locus_tag="OQN13_004092"
     CDS             454..>1421
                     /locus_tag="OQN13_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1617280.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDL"
BASE COUNT          382 a          329 c          339 g          371 t
ORIGIN      
        1 aaaatattac gcattattca caccaaaggt gtgtcattgc ctgatgatcg cgagtcagtc
       61 ttctggctga atattaaaaa tattccgcca tcagcgagca acaaagcgac gaactcgctg
      121 gaaatagcag taaaaacgcg tatcaagctg ttctggcgac cagcaaatat ccgccttatt
      181 cccgaagatg ctgcgccgaa agtgaaatgg cgccgggaag ggcgaaatct gatagcagag
      241 aatcccaacc caattcatat cagtgtgatg gatgtcatcg ttgatggcca tgatgtgccc
      301 ctcaatatga tccgtccttt tgaaacgctc acgttacctt tacctgctaa ttctgcggga
      361 ggccaaatga cctggcgatt tattaatgat tacggcgcag tcagtgaccc gataaaaatg
      421 acgctgtaag caagggataa ggataggcgt attatgatga cgaccagaat agtggttggc
      481 ctcacggcag ggacgtgtct gattttctcg caaaacctga tggccgaggt cagtgtattc
      541 aatccggcgc ttctggaaat cgaccatcaa tccggagtcg atattcgcca gtttaatcgg
      601 gcaaacctga tgcccccagg tgtttatagc gttgatattt ttatcaacgg taaaatgttt
      661 gaacgtcagg atgtgacatt tgttcaggat aatccagatg ctgatctgca cgcttgcttt
      721 attgccatta aaaaaacact gtcctccttt ggcataaaag ttgatgcgct caaatcgttc
      781 aatgatgtgg atgagacggt ttgcctcgat cctgctccac gtattgaagg ctcatcctgg
      841 cagtttgaca gtgataaatt gcagctgaat atatccattc cccaaatcta catggacgcg
      901 atggcttatg attacatcag ccccacgcgt tgggatgagg ggattaacgc gctcaccatc
      961 aactacgatt tttctggttc acatacacta cgttcagatt atggttcaca agagacagat
     1021 accagttatc tcaatctgcg caatggactg aatattggac cgtggcggct acgtaattac
     1081 agtactttaa acaccagcga tggccgtgcg gaatacaact ccattagtac ctggatacag
     1141 cgcgatattg ccgcgttaag aagccagatt atgattggtg atacgtggac ggcgagcgat
     1201 attttcgaca gtacgcaaat tcgcggcgcg cgtttgtata ctgataacga tatgctaccc
     1261 gccagccaga atggctttgc tcctgtggtt cgtgggattg caaagtccaa cgccaccgtc
     1321 atcattcggc agaatggcta cgtgatttat cagtctgccg ttccacaagg tgcttttgag
     1381 atcaccgatc tcaacaccgc cagtacaggt ggcgatttgg a
//
LOCUS       DAJVRF010000292         1407 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJVRF010000292 DAJVRF010000000
VERSION     DAJVRF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.296"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            30..758
                     /gene="mpaA"
                     /locus_tag="OQN13_004093"
     CDS             30..758
                     /gene="mpaA"
                     /locus_tag="OQN13_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCS1617281.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(733..>1407)
                     /gene="ycjG"
                     /locus_tag="OQN13_004094"
     CDS             complement(733..>1407)
                     /gene="ycjG"
                     /locus_tag="OQN13_004094"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCS1617282.1"
                     /translation="LAARKQQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLW
                     QAGAKLLKVKLDNHLISERMVAIRTAVPDATLIVDANESWRAEGVAARCQLLADLGVA
                     MLEQPLPAQDDAALENFIHPLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEA
                     LALATEARAQGFSLMLGCMLCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFT
                     TGELHL"
BASE COUNT          318 a          413 c          356 g          320 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
      361 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
      421 aacctggctc agaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
      481 cctgggtggt ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat
      541 taggcgaatg gctggcccag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa
      601 cgcctggctc tttcggcagt tggtgtgcag atctcaactt gcattgcatc accgcagagt
      661 ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc
      721 tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc
      781 taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa
      841 agcggcgcta atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg
      901 tgcacgcgct tcagtcgcca gcgccagcgc ttccgtcaga ccaccgattt tatcgagctt
      961 aatgttaacc atctcatagc gccctttcag cgccttcaga ttgctacgtg tatgacaact
     1021 ctcatcagca caaatcggca acggatgaat aaaattctcc agcgccgcat catcctgcgc
     1081 aggaaggggt tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc
     1141 caccccttct gcacgccagg attcatttgc atcaacgatc agcgtcgcat cgggcacagc
     1201 tgtgcgaatt gccaccatcc gctcactaat aagatggtta tccagcttca ctttcagtaa
     1261 tttcgcgcct gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat
     1321 gactaccgtc tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag
     1381 cgattgctgc tgttttcgcg cggccag
//
LOCUS       DAJVRF010000293         1394 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJVRF010000293 DAJVRF010000000
VERSION     DAJVRF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.297"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..1197
                     /locus_tag="OQN13_004095"
     rRNA            <1..1197
                     /locus_tag="OQN13_004095"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1292..>1394
                     /gene="rrf"
                     /locus_tag="OQN13_004096"
     rRNA            1292..>1394
                     /gene="rrf"
                     /locus_tag="OQN13_004096"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          341 a          316 c          434 g          303 t
ORIGIN      
        1 ctgatatgta ggtgaagtcc cttgcggatg gagctgaaat cagtcgaaga taccagctgg
       61 ctgcaactgt ttattaaaaa cacagcactg tgcaaacacg aaagtggacg tatacggtgt
      121 gacgcctgcc cggtgccgga aggttaattg atggggtcag cgcaagcgaa gctcttgatc
      181 gaagccccgg taaacggcgg ccgtaactat aacggtccta aggtagcgaa attccttgtc
      241 gggtaagttc cgacctgcac gaatggcgta atgatggcca ggctgtctcc acccgagact
      301 cagtgaaatt gaactcgctg tgaagatgca gtgtacccgc ggcaagacgg aaagaccccg
      361 tgaaccttta ctatagcttg acactgaaca ttgagccttg atgtgtagga taggtgggag
      421 gctttgaagt gtggacgcca gtctgcatgg agccggcctt gaaataccac cctttaatgt
      481 ttgatgttct aacgtggacc cgtgatccgg gttgcggaca gtgtctggtg ggtagtttga
      541 ctggggcggt ctcctcctaa agagtaacgg aggagcacga aggttggcta atcctggtcg
      601 gacatcagga ggttagtgca atggcataag ccagcttgac tgcgagcgtg acggcgcgag
      661 caggtgcgaa agcaggtcat agtgatccgg tggttctgaa tggaagggcc atcgctcaac
      721 ggataaaagg tactccgggg ataacaggct gataccgccc aagagttcat atcgacggcg
      781 gtgtttggca cctcgatgtc ggctcatcac atcctggggc tgaagtaggt cccaagggta
      841 tggctgttcg ccatttaaag tggtacgcga gctgggttta gaacgtcgtg agacagttcg
      901 gtccctatct gccgtgggcg ctggagaact gaggggggct gctcctagta cgagaggacc
      961 ggagtggacg catcactggt gttcgggttg tcatgccaat ggcactgccc ggtagctaaa
     1021 tgcggaagag ataagtgctg aaagcatcta agcacgaaac ttgccccgag atgagttctc
     1081 cctgaccctt taagggtcct gaaggaacgt tgaagacgac gacgttgata ggccgggtgt
     1141 gtaagcgcag cgatgcgttg agctaaccgg tactaatgaa ccgtgaggct taaccttaca
     1201 acgccgaaga tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga
     1261 acgcagaagc ggtctgataa aacagaattt gcctggcggc cgtagcgcgg tggtcccacc
     1321 tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc
     1381 ccatgcgaga gtag
//
LOCUS       DAJVRF010000294         1386 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJVRF010000294 DAJVRF010000000
VERSION     DAJVRF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.298"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..>1386)
                     /locus_tag="OQN13_004097"
     CDS             complement(<1..>1386)
                     /locus_tag="OQN13_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1617283.1"
                     /translation="SRNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFT
                     DCSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGD
                     LTAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYD
                     PVDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQ
                     WQYDDHGWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAY
                     SEQGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAY
                     ELATAWNTSGQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVH
                     TTAANLDIDIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAE
                     KTDRIPEGVIR"
BASE COUNT          206 a          472 c          395 g          313 t
ORIGIN      
        1 tccggataac cccttccggg atacggtccg tcttctccgc cagcctgccg tattcatcgt
       61 agcgatagac atagtgcgca tcttccgcga tgcggttatc cggccacgcc gtgagggtgc
      121 tgtccgggtg cagttccggg tccggcagcc gatttcctgc cgggtctgtt gcatacggga
      181 tatcgatatc caggttcgct gccgtggtgt ggacgcccgt cagcctcccc gtgtcgctgt
      241 aacggtactc ccggctctcc tgcgggccac tgatgcggat tagctgtccg ttgtcgttcc
      301 agtcgtagtc acggtcaagc tgcggaaggt tcaggtgcct gctccggagc tggccgctgg
      361 tattccaggc ggtggcaagt tcatatgcct ccccgccgaa gctgcgggcc gtctcacggt
      421 gcagccggtc ccgcgtgtac tcgaccagcg gtgttccgcc cagcttcatc cccgcaagat
      481 aaccgctgcc atacgtcagc cactccaccg gcggcaggcc gtccggctcc tgacgggtcg
      541 ccagcccctg ttcgctgtac gcgtgccccg tctcatgctc ccacagcatc tcccccgtct
      601 ccggggtctc caccgtctgc cgttcgcccg tcagacggcc tttatcgtca tagccgtagt
      661 ggaccgccac acggtggcct tcactcaggt ggctgatttc tgtcagccag ccgtggtcgt
      721 catactgcca ctgctccgcc ggttcaccgt tcaccgtgcg ccgggtgata cggtccgacg
      781 catcgtaatg ccagagggtg ataagcccct catcttcact ctgcgtgagt tttccggtca
      841 ggtcatactg gtaacgttgc gtccggccgt caaagccgcg ctgctccgtc agcctgtcga
      901 ccgggtcata ccggaacgtg gactggctgc cgttctcgtt ggtcagcaca gtgatgcgcc
      961 cggcggcgtc ataccccatg cttcgcgtca gaccgccctg cgtggtgctg acggcctttc
     1021 cccacgcatc atactgtatc tcactgcggc tgccgtccgg ggcaacgata gcggtgaggt
     1081 ctcctgcggc gctgtactca taacgggttt cacgcccctg cgcatccctc tgactgacca
     1141 gttgaccgcg cgggttataa ctgctgtaag tgctgatgcc ttcttcccgg tgaacggcga
     1201 tttgttgacc gtaccggtca tactcatacc gcgtcgtgta ccccgagcag tccgtaaacg
     1261 cctgaagctg accgtaccgg tttcgcgtta ttctccgcct caccccggtt ccgtcttcca
     1321 ccgcacaggg caggccactg cgggaagaat cataaaacca gcgcgtaatg ttaccgttgc
     1381 gcgagg
//
LOCUS       DAJVRF010000295         1380 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJVRF010000295 DAJVRF010000000
VERSION     DAJVRF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.299"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>1380
                     /locus_tag="OQN13_004098"
     CDS             <1..>1380
                     /locus_tag="OQN13_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCS1617284.1"
                     /translation="DRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDI
                     TIKGKTTSQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQC
                     YPNTALVGVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYS
                     NNMAWCLWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVTDGFGGTEPRITCNS
                     YLTTQRKAWDVLSDFCSAMRCMPVWNGQMLTFVQDRPSDKVWTYNRSNVVMPDDGAPF
                     RYSFSALKDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQ
                     AHRAGLWLIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQ
                     TRTLTLDREITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVW
                     GLKLPTLRQR"
BASE COUNT          305 a          367 c          444 g          264 t
ORIGIN      
        1 gaccgtctgc gcctgacctt cggtgtgcag gcactggtgg aaaccacctc aaagggtgac
       61 cggaatccgt cggaagtccg cctgctggtt cagatacagc gtaacggtgg ctgggtgacg
      121 gaaaaagaca tcaccattaa gggcaaaacc acttcgcagt atctggcctc ggtggtggtg
      181 ggtaacctgc cgccgcgccc gtttaatatc cggatgcgca ggatgacacc ggacagcacc
      241 acagaccagc tgcagaacaa aacgctctgg tcgtcgtaca ccgaaatcat cgatgtgaaa
      301 cagtgctacc cgaacacggc gctggtcggc gtgcaggtgg attcagagca gttcggtaac
      361 cagcaggtga gtcgcaatta tcatcttcgc gggcgcattc tgcaggtgcc gtcgaactat
      421 aacccgcaga cgcggcaata cagcggtatc tgggacggaa cgcttaagcc agcatacagc
      481 aacaacatgg cctggtgtct gtgggacatg ctcactcatc cgcgctacgg catggggaaa
      541 cgtcttggtg cggcagatgt ggacaaatgg gcgctgtatg tcatcggcca gtactgcgac
      601 cagtcagtga cggacggatt tggcggcacg gagccgcgca tcacctgtaa ctcttacctg
      661 accacacagc gtaaggcgtg ggatgttctc agtgatttct gctcggcgat gcgctgtatg
      721 ccggtatgga acggacagat gctgacgttc gtgcaggacc gaccgtcgga taaggtgtgg
      781 acctataacc gcagtaatgt ggtgatgccg gacgatggcg cgccgttccg ctacagcttc
      841 agcgccctga aggaccgcca taatgccgtt gaggtgaact ggattgaccc agataacggc
      901 tgggagacgg cgacagagct tgtggaggat acgcaggcca ttgcccgtta cggtcgtaat
      961 gtcacgaaga tggatgcctt tggctgtacc agccgggggc aggcacaccg agccgggctg
     1021 tggctgatta aaacggaact gctggaaacg cagaccgtgg acttcagcgt gggcgcagaa
     1081 gggcttcgcc atgtaccggg tgatgtcatt gaaatctgcg atgatgatta tgccggtatc
     1141 agcaccggtg gtcgcgtact ggcggtgaac agccagaccc ggacgctgac gctcgaccgt
     1201 gaaatcacgc tgccgtcctc cggtaccacg ctgataagcc tggttgacgg aagtggcaat
     1261 ccggtcagcg tggaggtcca gtccgtcacc gacggcgtga aggtgaaagt gagccgggtt
     1321 cctgacggcg ttgcagaata cagcgtgtgg gggctgaagc tgccgacgct gcgccagcgt
//
LOCUS       DAJVRF010000296         1380 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJVRF010000296 DAJVRF010000000
VERSION     DAJVRF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.300"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            4..984
                     /locus_tag="OQN13_004099"
     CDS             4..984
                     /locus_tag="OQN13_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCS1617285.1"
                     /translation="MVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVRS
                     IDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTPP
                     GYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETDA
                     KGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQG
                     GYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGDK
                     DVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            999..>1380
                     /locus_tag="OQN13_004100"
     CDS             999..>1380
                     /locus_tag="OQN13_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCS1617286.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLG"
BASE COUNT          359 a          315 c          365 g          341 t
ORIGIN      
        1 ttagtggtgg gtgttccggt tccggacaat attcacccga tgatcaaaga cgaagcagga
       61 gtctggtcgt ggcgcacgcc tgtcctgaaa ggcaacctgt atgagtattt tttcaatgtt
      121 gatggtgtac gcagcattga tataggcacc gcaatgacta agcctcagcg ccaggttaac
      181 tccagtatgg ttctggtgcc aggcagttat ctggatacgc gttctgttgc gcatggtgat
      241 ttgatcgcca taacttacca ctccaacgca ttgcaatctg aacgtcagat gtatgtctgg
      301 accccgccag gatacaccgg catgggcgag cctttgccag tgctctattt ctatcacggt
      361 tttggtgata ccggacgttc cgctatcgat caggggcgta tcccgcaaat catggataac
      421 ctgctggctg aagggaaaat taaaccgatg ctggtggtga tcccggatac cgaaaccgat
      481 gcgaagggca ttattcccga agatttcgtg cctcaggaaa gacgtaaagt cttttatccg
      541 ctgaatgcta aagcggcaga tcgcgaactg atgaacgata ttatcccgct gattagcaag
      601 cgttttaatg tccgtaaaga tgccgatggt cgcgcgctgg cagggctttc acaaggcggg
      661 taccaggcgc tggtttccgg aatgaatcat ctggaaagct ttggctggct ggccacattc
      721 agtggtgtta ccacgacaac cgtaccggat gaaggtgtcg cggcccggct gaacgatccg
      781 gcagctatta accagcaact acgtaatttt actgtggtcg ttggtgataa agatgtcgta
      841 accggcaagg atatcgccgg gctgaaaact gagcttgagc agaaaaaaat taagtttgat
      901 taccaggaat atcccggcct gaaccatgaa atggatgtct ggcggcctgc ctatgcagcc
      961 ttcgtacaga aattatttaa ataaaataag gcattaagat gaaattaatc attaccgaag
     1021 attaccagga aatgagccgt gtcgcggcac accatctgct tggttatatg tcgaagacgc
     1081 gtcgtgttaa cctggcaatt accgccggta gcacgcccaa aggcatgtat gaatacctca
     1141 ccaccctggt taaaggtaag ccctggtacg ataactgcta tttctataat ttcgatgaaa
     1201 ttccctttcg cggcaaagag ggagaaggcg taacgattac caatctgcgt aatctgtttt
     1261 tcacccctgc ggggatcaaa gaagagaata tccagaagct cactattgat aactaccgcg
     1321 agcatgatca gaaactggcg cgtgaaggcg gcctggattt ggtggtgctg ggattaggtg
//
LOCUS       DAJVRF010000297         1355 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJVRF010000297 DAJVRF010000000
VERSION     DAJVRF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.302"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..296)
                     /locus_tag="OQN13_004101"
     CDS             complement(<1..296)
                     /locus_tag="OQN13_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HCS1617287.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIA"
     gene            complement(418..768)
                     /locus_tag="OQN13_004102"
     CDS             complement(418..768)
                     /locus_tag="OQN13_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1617288.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(811..1275)
                     /locus_tag="OQN13_004103"
     CDS             complement(811..1275)
                     /locus_tag="OQN13_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCS1617289.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(1272..>1355)
                     /locus_tag="OQN13_004104"
     CDS             complement(1272..>1355)
                     /locus_tag="OQN13_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCS1617290.1"
                     /translation="TYAGGKQWKGLMTRREIEREVCLWGQQ"
BASE COUNT          280 a          393 c          348 g          334 t
ORIGIN      
        1 gcaatatttt tcacggcacg tcgccagaac tcataggcca cgcaccaccg ctcaagtacc
       61 gccaggtcag tcacgcacag caggccctga ccgcagagtt ctttggttgt cagttgccac
      121 atgatcgtgg cgagagggag atcttcttca gcgaaccact ctggtggctc aacacctttg
      181 atgggcgtaa aaacaggttc atctttgttc agggctcgct tgccggggtt tccggccagc
      241 gccttgcgcg ccgttggctt ggggcgacgc ccggaacgcc ccgccgttcc agccatatgc
      301 ggcactcctg gttaaatttc atttttcgcg ggtataaaaa aacgatgggg cgggcagtcc
      361 ggaagacgtc aggtcacagg gatttgcccc gcccctccct acaagtgaga ataattatca
      421 ccggattcgt tcgcgcgctg ttttcgcttt atggcagggc cagcacagac tctgcaggtt
      481 gctgtctgcg tctgttccgc catgcgcttt cgggatgatg tggtcgacgg ttttcgcctc
      541 gctcaccaca ccgacacgca gacacaactg acacagacct ttatcgcgct tcagaatacg
      601 ggcacgaatc accgtccatt ttgagccata gccacgctgg tggcggctca gtccgcgctg
      661 gtgctgcacc cagccttcac cgcgatgttt atcgcagtaa cccgaactgt ctgtggttgt
      721 acctgcacat ccacgcttac ggcaggcacg tgggattagt gatggcataa atacctcata
      781 ccctgcgaaa tgtttaccac gataaaaagg ctacttaatg cactgagtgc ggatatactc
      841 ctgtgcccct tccagttgca tctgcatcgt catcagccgc tctctgaggg tgaaataatc
      901 ccgttcagcg gtgtctgcca gtcgggggct ggttgcatta tccacgccgg aggcggtggt
      961 ggcttcacgc actgactgac agactgcttt gatgtgcaac cgacgacgac cagcggcaac
     1021 atcagcgcgc agagtttcat tttcagcttt cgcatcagct aactccttcg tgtatttagc
     1081 atcgagtgca ttaacatcac gctgtctctg ctgcatgtca gtaatggttg cgttcgccag
     1141 cttcagttct cttgcgtttt tgtcgcgctg ctctttgtag gtaatggcgt tatcacggta
     1201 atgattaaca gcccatgaca ggcagacgat gatgcagata accagagcgg agataatcgc
     1261 ggtgactctg ctcattgctg cccccacaaa cagacttcac gctcaatctc acgacgggtc
     1321 atcagccctt tccattgctt accgccagca tatgt
//
LOCUS       DAJVRF010000298         1355 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJVRF010000298 DAJVRF010000000
VERSION     DAJVRF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.303"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            89..>1355
                     /gene="istA"
                     /locus_tag="OQN13_004105"
     CDS             89..>1355
                     /gene="istA"
                     /locus_tag="OQN13_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HCS1617291.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRY
                     EPVINDSYQALAEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRA
                     LNARLRELLTDMNNRPMKGYGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPD
                     YHVEYARHWYSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHM
                     PERHRRHGTWTPERLIEQGNRTGPSTGRVV"
BASE COUNT          345 a          377 c          345 g          288 t
ORIGIN      
        1 ggaattaact cttttcacca cagtctccat tgccgtgttt aaaccagtgg agacgtcaat
       61 gtccaaaaag agaaaatcca ggagcactat ggaaacttgt tttaagatcc ttcagctcaa
      121 gttcgataag aagctgacta accgttgtat cggactgacg ttacacatca gcgcctcaac
      181 cgtttttgag gttcttgccc gcttcaaagc ctcttctctg tcatggccat tacctgcgga
      241 tatatcccat gacacgctgg agaaactcat tttcccgccc aaagacacct ctgcgtcaga
      301 gcttgttatg cctgacatgc tgtacttcga tacggaaatg agaaaacctg gcgtgacgcg
      361 ccaactgttg tggatggaat acaaggcaca ggcgggtgac aaagcgatgg gatactcaca
      421 cttctgccgt tgctaccgga agtggaaaaa aacgcgacgc ctctccatgc gacaggagca
      481 tcgtgctggc gaaaagctct ttatcgactt ctgtggtcct acggtccctg ttattaatcc
      541 ggacaccgga gagatacgcc gcgtggcaat cttcgtggct gtaatggggg cgtccaacta
      601 tacctatgtt gaagcgtgcg aaggtcagga catgatgtca tggttaaatg cccacagccg
      661 ttgcctgacc ttcctgggcg gcgtaccgaa gctgctcatc cccgataacc tcagaagcgc
      721 agtgaaaaaa gccgatcgct atgagcctgt catcaacgac agttatcagg cgctggctga
      781 gcactatggc acggttatta tcccggcccg cccgcgcaaa cctaaagata aaccaaaagc
      841 agaaaacggc gtcctcatcg tcgagcgatg gcttcttgcc cgcatccgta atgaaacctt
      901 ccacacgctc agggcgctga atgcacgcct tcgggagttg ctgacggaca tgaataaccg
      961 accaatgaag ggatacggta atcaaacccg cgcagaacgc ttccgcatgc tggatgcacc
     1021 agcactctct ccgctgcctc tggagcctta tgaatacacg gaatacaaag ccgtgaaggt
     1081 tggccctgac taccacgttg aatatgcccg tcactggtac tccgttccac acgaactggt
     1141 cggtcagcgt ctgtcactga aggtggggca gtcggtggta cagctctggc acaaggggca
     1201 gtgtgtggcg caacatccgc gcagcacaca tgaatataaa cacacaacca atccgcttca
     1261 catgccagaa cgccaccggc ggcatgggac atggacaccg gaacgcctta tcgagcaggg
     1321 aaaccgtaca ggtccgtcca caggccgggt ggttg
//
LOCUS       DAJVRF010000299         1335 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJVRF010000299 DAJVRF010000000
VERSION     DAJVRF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.304"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..190
                     /gene="bdm"
                     /locus_tag="OQN13_004106"
     CDS             <1..190
                     /gene="bdm"
                     /locus_tag="OQN13_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCS1617292.1"
                     /translation="STAEPALVNAIEQGLRAEQGVVTEDDILMELTKWVEASDNDILS
                     DIYQQTINYVVSGQHPTL"
     gene            292..429
                     /gene="sra"
                     /locus_tag="OQN13_004107"
     CDS             292..429
                     /gene="sra"
                     /locus_tag="OQN13_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCS1617293.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            586..>1335
                     /gene="maeA"
                     /locus_tag="OQN13_004108"
     CDS             586..>1335
                     /gene="maeA"
                     /locus_tag="OQN13_004108"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS1617294.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDIGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQ"
BASE COUNT          375 a          319 c          319 g          322 t
ORIGIN      
        1 ttcaacagca gaacccgctc tggttaatgc cattgagcaa ggccttcggg cagagcaagg
       61 tgttgtcact gaagatgaca ttctcatgga gctgaccaaa tgggtggaag cttctgataa
      121 cgacatcctc agtgatatct accagcaaac gatcaattat gtggtcagtg gccagcaccc
      181 tacgctttaa ggtgctatgc ttgatcggca acctaattta ggggtttagc acgtgtttct
      241 tcgctacggc gatgttgtcc ttaaaactag caacaggatt gaggagttaa aatgaaatcg
      301 aaccgtcagg cacgtcatat tcttggactg gaccataaaa tttctaacca gcgcaaaata
      361 gttaccgaag gtgacaaatc cagcgtggta aataacccaa ccggcagaaa acgccccgct
      421 gaaaagtaat tcataaccat cagtcctcaa tgacgattaa acaccattgc ctgcgcaatg
      481 gtgtttttgt ttttatctgc tttatacttg gggccgacgc cctggcggta aagcaaagac
      541 gataaaagcg tgccagggat ggatattcaa aaaagagtga gtgacatgga accaaaaaca
      601 aaaaaacagc gttcgcttta tatcccttac gctggccctg tactgctgga atttccgttg
      661 ttgaataaag gcagtgcctt cagcatggaa gaacgccgta acttcaacct gctggggtta
      721 ctgccggaag tggtcgaaac catcgaagaa caagcggaac gagcatggat ccagtatcag
      781 ggattcaaaa ccgaaatcga caaacacatc tacctgcgta acatccagga caccaacgaa
      841 accctcttct accgtctggt aaacaatcat cttgatgaga tgatgcctgt tatttatacc
      901 ccaaccgtcg gcgcagcctg tgagcgtttt tctgagatct accgccgttc acgcggcgtg
      961 tttatctctt accagaaccg ccacaatatg gacgatattc tgcaaaacgt accgaaccat
     1021 aatatcaaag tgattgtggt gactgacggt gaacgtattc tggggcttgg tgaccagggc
     1081 atcggcggga tgggcattcc gatcggtaaa ctgtcgctct ataccgcctg tggcggcatc
     1141 agcccggcgt atacccttcc ggtggtgctg gatatcggaa cgaacaacca acaactgctt
     1201 aacgatccgc tgtatatggg ctggcgtaat ccgcgtatca ctgacgacga atactatgaa
     1261 ttcgttgatg aatttatcca ggctgtgaaa caacgctggc cagacgtgct gttgcagttt
     1321 gaagactttg ctcaa
//
LOCUS       DAJVRF010000300         1331 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJVRF010000300 DAJVRF010000000
VERSION     DAJVRF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.305"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..174
                     /locus_tag="OQN13_004109"
     CDS             <1..174
                     /locus_tag="OQN13_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617295.1"
                     /translation="IIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKR
                     RCQQQFTSQEVQN"
     gene            184..>1331
                     /gene="yicI"
                     /locus_tag="OQN13_004110"
     CDS             184..>1331
                     /gene="yicI"
                     /locus_tag="OQN13_004110"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCS1617296.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          332 a          315 c          349 g          335 t
ORIGIN      
        1 atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag cgcgattatc
       61 gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga acagctggct
      121 cagggtaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa ctaaggaacg
      181 gcaatgaaaa ttagcgatgg aaactggttg attcaacctg gcctcaattt gattcacccg
      241 cttcaggtgt tcgaggttga acagcaggat aatgaaatgg tggtctatgc tgccccccgt
      301 gatgtgcgtg aacgtacctg gcagcttgat acgcctttat ttacgctgcg ctttttctcc
      361 ccacaggaag gtattgtcgg tgtgcggatt gagcattttc agggggcgct gaataacggt
      421 cctcattatc cgctcaatat tttgcaggac gtgaaggtca caatcgaaaa cacagaacgt
      481 tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga gttctggtca
      541 ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa taatggctac
      601 gtgcaggaca cgaataatca acgcaattat atgtttgagc ggcttgatct tggcgttggc
      661 gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg ccagacggta
      721 gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa tatcccgttc
      781 tacatgacta accgtggtta tggggtactg gtcaatcatc cccagtgtgt ctcttttgaa
      841 gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata tctcgaatac
      901 tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg ctttactggt
      961 cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc atttaccacc
     1021 aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg caatctgccg
     1081 ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg cgattttgag
     1141 tgggacccgc tgactttccc tgacccggaa gggatgatcc gccgcctgaa agcgaaaggg
     1201 ctgaaaatct gcgtctggat taacccctat atcggtcaaa aatcccccgt ctttaaagag
     1261 ttacaagaga aaggctattt actcaaacgc ccggacggtt cgctatggca gtgggataaa
     1321 tggcagccag g
//
LOCUS       DAJVRF010000301         1272 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJVRF010000301 DAJVRF010000000
VERSION     DAJVRF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.306"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..439
                     /locus_tag="OQN13_004111"
     CDS             <1..439
                     /locus_tag="OQN13_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCS1617297.1"
                     /translation="EHGLTRGALLDYHSRYKLVFLAHSQPEYRKLGPFVADIHQWQNL
                     DDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGYFRPHIDSTERQQLAALIDSYRRGE
                     QPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRVINLRHSGVL"
     gene            436..>1272
                     /locus_tag="OQN13_004112"
     CDS             436..>1272
                     /locus_tag="OQN13_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCS1617298.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWR"
BASE COUNT          277 a          325 c          351 g          319 t
ORIGIN      
        1 agaacatggc ttaacgcgcg gggcgcttct cgattatcac agccgctata aactcgtctt
       61 tctggcgcat tctcagccgg agtaccgcaa acttggcccg ttcgtggctg atattcacca
      121 gtggcaaaat ctggatgact attacaacca gtaccgccaa cgcgtagttg ttttgctttc
      181 tcaccccgcc aacccgcgcg atcacaccaa tgttttgatg cacgttcagg gttattttcg
      241 cccgcatatt gattccacag aacgccagca gctggctgcg cttatcgaca gttatcgccg
      301 tggcgagcaa ccacttcttg cgccgctgat gcgtatcaaa cactatatgg cgctttatca
      361 tgacgcctgg ctttcagggc agcgttattt cgaactttgg ccgcgtgtga ttaacttgcg
      421 ccattcagga gttttatgac tacccatctg gtctggtttc gccaagattt acgtctgcac
      481 gataatctcg cactggctgc cgcctgccgc aattcgtctg cacgcgtgct ggcgttgtat
      541 atcgctacac cacgccagtg ggcgacgcat aacatgtcgc cgcgtcaggc tgaacttatc
      601 aatgctcaac tgaatgggct acaaatagcg cttgcggaaa aaggtattcc tttattgttc
      661 cgtgaagtgg atgactttgt cgccagtgtc gaaatagtta aacaggtgtg cgcggaaaac
      721 agcgttaccc acctgtttta taactatcag tatgaagtga atgagcgggc gcgggatgtg
      781 gaagttgaaa gagcgctgcg taacgtggtg tgtgaaggat ttgatgacag cgtgatcctg
      841 ccgcctggcg cggtgatgac cggtaatcac gagatgtaca aagtctttac gccttttaag
      901 aatgcctggc tgaaacggct gcgggaaggg atgccggagt gcgtcgctgc gccaaaagtt
      961 cgtagtagcg gatcgataga gccctcgcca tccattacgc tgaattatcc tcgtcagtct
     1021 ttcgatactg cgcattttcc ggtggaagaa aaagcggcga ttgcgcaatt acgccagttt
     1081 tgccagaacg gtgccggaga atatgagcaa caacgagatt ttccggcagt ggaaggcacc
     1141 agccgtttgt cggccagcct ggcaacgggc gggttatcgc ctcgccagtg cttgcatcgc
     1201 ttgttggctg aacagccgca ggcgctggac ggtggggtcg gtagtgtctg gcttaatgag
     1261 ctgatctggc gc
//
LOCUS       DAJVRF010000302         1264 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJVRF010000302 DAJVRF010000000
VERSION     DAJVRF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.307"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(23..>1264)
                     /locus_tag="OQN13_004113"
     CDS             complement(23..>1264)
                     /locus_tag="OQN13_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1617299.1"
                     /translation="IKHIIIAERAHQESLVKQKRIAQVWNHKTQLARELKKPMGKQAP
                     GWLELSEDGSYYIVDEDKASLVNIIYDKRLSGMSMFAICKWLNEQGYPTINQRKVRIS
                     KTKKPDGNWSALSVKHILTSRSVLGYLPAKISTEDRKTVLREEIEGFYPQIVTDSKFY
                     AVQQLLEETGKGKTSSGEHWLYVNILKGLIRCKCGLVMTPTGIRKPVYQGTYRCNGNK
                     ESRCSYGTVSRKLLDTQLCSRLFSKLSQLHDEATDTAKLDELQRRLNTVDGELEKLTE
                     TLIQLPNITQIQEALRVKQEEKDELIVQLSREKARVKSVSSLDLSGLDMESVEGRTEA
                     QIIIKRLVKEIVVSGNEKLVDIYLHNGNMIRGFPLDGKDDHTLTLEEATDEMQSLDDM
                     LIFGEPVTRIYPAGDMEEVDA"
BASE COUNT          333 a          298 c          228 g          405 t
ORIGIN      
        1 cagtgaaccc taacaaaaaa tactaagcgt ccacctcttc catatcacca gcaggataaa
       61 tccttgttac tggttcacca aaaataagca tatcatctaa tgactgcatc tcatcggttg
      121 cttcttcaag cgttaaggtg tggtcatctt taccatcaag aggaaaacca cgaatcatat
      181 ttccattgtg aagatatata tccaccagct tttcattacc agaaacaaca atctctttca
      241 ccagacgctt aataataatc tgggcttcag ttctcccctc tacagactcc atatcaagac
      301 cgcttaaatc caagcttgat acagatttta ccctcgcctt ttctcgtgat aactgaacta
      361 tcagttcatc tttttcttcc tgtttaaccc gtagtgcttc ttgaatctga gtgatgttag
      421 gaagctgtat cagcgtctca gtgagttttt ccagttcacc atcaacggtg tttaatcttc
      481 tttgtagttc atctaattta gctgtatcgg tagcttcatc gtgtagctgt gacagtttgc
      541 taaacagacg actacaaagc tgtgtatcaa gtaactttct ggacactgta ccgtagctac
      601 aacgggactc cttattacca ttgcaacggt aagtaccttg ataaacgggt ttacgtatcc
      661 ctgtaggagt cattacaagc ccacacttac aacggataag ccccttcagt atgttcacat
      721 atagccagtg ttcaccgctg gatgttttcc ccttccctgt ttcctctaac agttgctgta
      781 cagcgtaaaa cttagagtct gtgacaatct gaggatagaa cccttcaatt tcttcacgta
      841 acactgtttt acggtcttct gtagaaatct tagcgggaag gtaacctaat acacttcgtg
      901 aagtcaggat gtgtttaacg cttaatgctg accagttgcc atccggtttc ttagtcttgg
      961 atattctgac cttacgctga ttaatggtcg ggtaaccttg ttcgtttagc catttacaga
     1021 tagcaaacat cgacatacca gaaagacgct tgtcatagat gatattcacc agtgacgctt
     1081 tatcttcatc aacgatgtag tatgaaccat cttctgatag ctctaaccat ccgggggctt
     1141 gcttacccat aggcttctta agttctcttg caagctgtgt tttatggttc cagacctgag
     1201 ctatacgctt ttgcttaaca agagattctt ggtgtgctct ctcagctatg atgatgtgct
     1261 taat
//
LOCUS       DAJVRF010000303         1262 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJVRF010000303 DAJVRF010000000
VERSION     DAJVRF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.308"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..183)
                     /locus_tag="OQN13_004114"
     CDS             complement(<1..183)
                     /locus_tag="OQN13_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617300.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLT"
     gene            complement(1004..1216)
                     /locus_tag="OQN13_004115"
     CDS             complement(1004..1216)
                     /locus_tag="OQN13_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HCS1617301.1"
                     /translation="MLCFLIYITLPFSQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
BASE COUNT          429 a          244 c          236 g          353 t
ORIGIN      
        1 cgtcaggtag gccattttgt ccagcacctg cgcaaattca agggagagaa actgctggct
       61 gaccagagag agatcgaggg aaaagagcga agagagtgac tggttcaaat gaaaggaaac
      121 caccgcaaaa gcatccggtg gcaggccgtc cacttccagt gtgaaacgta atccggttga
      181 catagcaacc tccgtgaaaa tgaataataa tccgcacata ttgcatgtac aacatgtcgg
      241 gcggatgaga taagcgagtc gacatctggc gttaccgggg aatatcagaa aaatgtcatg
      301 gttatgtgat gatttagtct gggattttac ttcagagaaa cgggtgtctg tttagtcaac
      361 aaaataatca caaacgcaac ggatgggttc tcgacgttat tcacgacagg ctcgtacatt
      421 acagcgagta ataaaaaacc tgatatgcaa agtcaaacca atattcataa tgtcttttta
      481 ttcacaacaa taaataattc aataacaatc attgaaatga atgatggttt gtataaggaa
      541 tatacgctac cgcccaccct gataacatga tctgcacatt gccatcttaa cgtcgttaaa
      601 cactggctta atagtggata tttcaaatta ggaatttatt aaattcacct gatgaatata
      661 ataagcaaat agcatagaaa ataatggcaa ctccccccga gaggaccatt tccaaagcac
      721 tgcaccacca cctggtgccc ggatgatctt gcttcagtgg taagagtacc tgattcattg
      781 ttcccaaaat gatgttttcc ccggccccgc caataatgtg cagctctcac gtgataatct
      841 attgattagg ttatattaag aatcaatatt taaaatacat ccattttctt gaataaatca
      901 acatatttat ttatcaattg ctgttttagc tctgactcat caagctctcc atatttatcc
      961 tatagataat tgcgaataaa tacagagaac gccatttatc cttttagcat acctgccgga
     1021 tatttttatt tgcattaata ttaattgcat ctccaactcc gagcatatcc atcattaaaa
     1081 actttttgag attatagaat acctgtgata acaagtgaaa catctgcaca atgtgaatag
     1141 ttagtttctt ttcagaggaa atgaaataga caagctgact gaaggggaga gtgatataaa
     1201 tcaaaaaaca taacatgaat ttaattaatt acgcccatca ttgtggataa agttactgaa
     1261 ta
//
LOCUS       DAJVRF010000304         1250 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJVRF010000304 DAJVRF010000000
VERSION     DAJVRF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.309"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            1..1068
                     /locus_tag="OQN13_004116"
                     /pseudo
     CDS             1..1068
                     /locus_tag="OQN13_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          345 a          252 c          382 g          271 t
ORIGIN      
        1 cccaacagac aagaatccag acggtatacc agccgggaag cttcacgccg ctcctgagaa
       61 tcgaaacaga gaatggcgaa caggcgaagg cgcggcaccg gagcctggcg gaggtgttgc
      121 aggaggacac gggggtgacg cttccggcgg agctggcggt gatgctgggg aggctggagc
      181 gggaactgcg tgcgggtgcg gtgagtgcgg agagtgaggc atggcttgcg cagtgcgggc
      241 tgacggtgga gcagatggaa agccagatgg aggcggaata catcccggag cggaggctgc
      301 atctttacca ctgtgaccac cggggactgc cgcaggcgct catcagcccg gaaggggaaa
      361 cggcgtggcg cggggagtat gacgagtggg ggaaccagct taatgaggag aacccgcatc
      421 acctgtacca gccgtaccgt ttgccggggc agcagtatga tgaggagtcg ggactgtatt
      481 acaaccgtca ccggtactac gatccgttgc aggggcggta tatcacccag gatccgatag
      541 ggttgaaggg gggaataaat ctatacacgt atccactggc accaatacgt tacaccactg
      601 gggttagaac gagttataag tgtatatggg cctcccgctc cagaccgggc aggtgctgaa
      661 actcctcttg ttcttactga tatgactgga ggagtgacta tttattatga tcctgaaaca
      721 ggtgattcaa tgacttttga tagtagcaat agaattgatc gtcgttctca acgtggtgct
      781 ggtgatccat atacaggtga agtcgttggt tgtgagacaa atggatcagg aatatcggca
      841 gcatatggca caacgaagat ctatactaca gatactagag caagatggct acatggtggg
      901 ggatcatcgt tgcgcgatcc ttatgctcct cgacaaggct ggaaaccaac tatgggatgt
      961 acccgtgcac agaatgaaga tgtagatgag ttgtgtaaga aagtgacaag ttggatgtat
     1021 tcgcatcctg gagagagaat tagatatgaa cgttttaaga cacgttaatg cgattaacat
     1081 gggagtgtgc attctaattt tggttattgg atgtgcaaat gctaaagttc ttaaacgatg
     1141 tatattaccc gctcctgatg gtggcgtaga aaaactatca aaacccaact tacatggagt
     1201 aattaaaaaa gttgatttga acacaaatga agcggaaatt aaaataaaag
//
LOCUS       DAJVRF010000305         1245 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJVRF010000305 DAJVRF010000000
VERSION     DAJVRF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.310"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..604
                     /gene="fhuF"
                     /locus_tag="OQN13_004117"
     CDS             <1..604
                     /gene="fhuF"
                     /locus_tag="OQN13_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCS1617302.1"
                     /translation="PNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGLMVPP
                     LMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETLISQA
                     LVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEKTLTN
                     GEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(642..>1245)
                     /gene="bglJ"
                     /locus_tag="OQN13_004118"
     CDS             complement(642..>1245)
                     /gene="bglJ"
                     /locus_tag="OQN13_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCS1617303.1"
                     /translation="SLFRKFAGNPYKLHTYTSQESFQDAMSRISFAAVIFSFSAMRSE
                     RREGLSCLTELAIKFPRTRRLVIADDDIEARLIGSLSPSPLDGVLSKASTLEIFHQEL
                     FLSLNGVRQATDRLNNQWYINQSRTLSPTEREILRFMSRGYSMTQIAEQLKRNIKTIR
                     AHKFNVMSKLGVSSDAGLLEAADILLCMRHCEASNVLHPY"
BASE COUNT          333 a          319 c          297 g          296 t
ORIGIN      
        1 accgaatgcg ctaagttctc tactcgcggt ctattccgat catatctatc gcaaccaacc
       61 aacgatgatc cgcgagaaca aaccgctgat ctcactatgg gcacaattgt atatcggcct
      121 gatggtgcca ccattaatgc tggcgctact gacgcaggaa aaggcattag atgtgtcgcc
      181 ggaacatttc catgctgagt ttcacgaaac cggacgcgtc gcctgtttct gggtcgatgt
      241 gtgtgaagat aaaaacgcaa caccacattc gccgcagcag cgaatggaaa cgttaatcag
      301 ccaggcgctg gttccggttg tgcaggcact agaagcgacc ggagagatca acggcaaact
      361 tatctggagt aataccggtt atttgatcaa ctggtatctc actgagatga agcaactgct
      421 cggcgaggcg acggttgaat cgctgcgcca tgccctcttt tttgagaaaa cgctcacgaa
      481 cggtgaagat aatccactct ggcgtaccgt cgtgctgcgc gatggcctgc tggtgcgccg
      541 aacctgctgc cagcgttatc gcttaccgga tgtgcagcaa tgtggcgatt gtacgctgaa
      601 atgaaagcac tgccggggaa gtaaacccgg catcatgcgg attaataggg atgcaacaca
      661 ttacttgctt cgcaatgccg catacataac agaatatctg cggcctccaa caaccctgca
      721 tcagaactga cgcccagttt cgacatcaca ttaaatttat gtgcacggat cgttttgata
      781 ttgcgtttaa gctgctcggc aatttgtgtc attgagtagc cacgcgacat aaagcgcaat
      841 atttctctct ccgtcgggct tagcgtccgg ctttggttaa tgtaccactg attgttcagt
      901 cggtcggtcg cctgacgtac accatttaat gacaaaaaga gttcctgatg aaaaatctcc
      961 agcgttgacg ctttacttaa tacaccgtcc agcggtgatg gcgacaatga accaatcagc
     1021 cgagcttcaa tatcatcatc cgcaataact aaacgccggg tacgcggaaa cttaatcgcc
     1081 agttcagtca ggcaagataa tccctctctg cgctcacttc tcatggcaga aaaagaaaaa
     1141 atgaccgccg caaatgagat ccgcgacatg gcatcctgaa atgactcctg actggtatag
     1201 gtatggagct tataagggtt acccgcaaac tttctgaata aactc
//
LOCUS       DAJVRF010000306         1231 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJVRF010000306 DAJVRF010000000
VERSION     DAJVRF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.311"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..710
                     /locus_tag="OQN13_004119"
     CDS             <1..710
                     /locus_tag="OQN13_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS1617304.1"
                     /translation="RRLTPPHVTAPFRTVAEWREWQLAEGQKRSEEINRLNRQLRVEK
                     ILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCTNFAFSGKPGTGKN
                     HLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRELCEVDLLVLDEIG
                     IQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERVMDRMVMNGGRWVN
                     FNWESWRPNVSHSRVVK"
     gene            725..1147
                     /locus_tag="OQN13_004120"
     CDS             725..1147
                     /locus_tag="OQN13_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCS1617305.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
BASE COUNT          318 a          272 c          373 g          268 t
ORIGIN      
        1 tccgccgact gaccccgcca catgtaaccg ccccattcag aacggtagcg gagtggcgcg
       61 agtggcaact tgctgaaggc cagaaacgta gcgaggagat caaccgcctg aatcgccagt
      121 tgcgggtgga aaaaattctg aatcgctcag gcatccagcc gttgcaccgt aaatgctcgt
      181 ttgcgaatta ccaggtgcag aacgacggcc agcgatacgc gttaagccag gcgaaatcca
      241 tcgccgatga actgatgacc gggtgtacaa attttgcgtt cagcggaaaa cctggtaccg
      301 ggaagaatca cttagcggca gctatcggga atcgcctgct gaaagacggt cagacagtga
      361 ttgtggttac cgtggctgat gttatgagcg ccctgcacgc cagctatgac gacgggcagt
      421 caggcgaaaa atttttgcgg gagctgtgcg aagtggatct gctggttctt gatgaaattg
      481 gcattcagcg cgagacgaaa aacgagcagg tggtgctgca ccagattgtt gatcgccgga
      541 cagcgtcgat gcgcagcgtg gggatgctga caaacctgaa ctatgaggtg atgaaaacat
      601 tgctcggcga gcgggtgatg gatcgcatgg tcatgaacgg cgggcgctgg gtgaatttta
      661 actgggagag ctggcgtccg aatgttagcc attcgagggt tgttaagtag tttcaggagg
      721 atttatggcg aaacctttta ctcccgaaca gcgggaagaa ctgaagacgc gaattgtgga
      781 actcgtgcat caggacggtc gggtcacgat tcggcagttg tcagatgaaa caggtatcag
      841 tcgtgcgtct gtcggtcgct tatgcataga actggtcgca agtggtgatg tatataattc
      901 tggctacggc ttattcccgt ctgaacaggc tcgcaaggac tggcaaagcg cccgcaaaaa
      961 actctcgaga gtaaaggtga ggaaaccggt tgttgttgat ccggacctta tctggtcatt
     1021 acctgacgga gaaatacgcc gctacgacag gcgcctgaat ataatctgtc gcgagtgccg
     1081 gaagagcgaa gctatgcagc gtgtactggc tttctatcag ggtaattttc aggaggcgat
     1141 actgtgaatg aaattagcta tcaggcttca attaccgctg gcattcgcat caaaggagag
     1201 gagcatggaa ataaaaccag aagatgagtt a
//
LOCUS       DAJVRF010000307         1230 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJVRF010000307 DAJVRF010000000
VERSION     DAJVRF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.312"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..1008
                     /gene="istA"
                     /locus_tag="OQN13_004121"
     CDS             <1..1008
                     /gene="istA"
                     /locus_tag="OQN13_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HCS1617306.1"
                     /translation="KPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPK
                     RKMRPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPK
                     QDAEHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLP
                     RACRPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQF
                     KETPEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVS
                     IRISLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEEL
                     L"
     gene            1008..>1230
                     /locus_tag="OQN13_004122"
     CDS             1008..>1230
                     /locus_tag="OQN13_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS1617307.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDF"
BASE COUNT          304 a          302 c          355 g          269 t
ORIGIN      
        1 aaaccgttta tggattacat cgacatgcgc ctggcagaga atgtctggaa tagcgaggtt
       61 atcttcgcgg agattaaggc aatgggttat acgggcggac gttccatgct gcgttactac
      121 atccagccca aacgtaaaat gcgtccgtca aaaagaacag ttcgcttcga aactcagcct
      181 ggataccagc tccagcatga ctggggcgaa gttgaggtgg aggttgccgg gcaacggtgc
      241 aaagttaact ttgcggttaa tacgctgggg ttctcccgca gcttccatgt cttcgccgca
      301 ccaaaacagg atgctgagca tacctacgaa tcactggttc gcgccttccg ctacttcggt
      361 ggttgtgtga aaacggtgct ggttgataac cagaaggctg cggtgctgaa gaataacaac
      421 gggaaagtcg tgttcaactc cggattcctg ttgctggctg accactataa cttcctgcca
      481 cgggcatgcc gtccacgcag ggccagaaca aaaggtaagg ttgagcggat ggtgaaatac
      541 ctcaaggaga acttcttcgt ccggtaccgc aggttcgaca gcttcactca tgtcaatcaa
      601 caactggagc aatggatagc cgatgtggct gacaaacggg agcttcgcca gttcaaagaa
      661 acgccggaac agcgcttcgc gctggagcag gaacatctgc agccgttacc ggatacagac
      721 ttcgatacca gttacttcga tatccgccat gtgtcctggg acagctatat cgaggttggt
      781 ggtaatcgtt acagcgttcc cgaagcgctg tgtggtcagc cggtatcgat acgaatatcg
      841 ctggatgacg agttgcggat ctacagtaat gagaaactgg tggcctcaca tcgcctctgt
      901 tcagcatcgt ctggctggca gacagtgccg gagcatcacg ccccgctctg gcagcaggtc
      961 agtcaggtgg aacatcgacc actgagtgcc tatgaggagc tgttgtgatg catgaactgg
     1021 aagtcctgct gagtcgcctg aaaatggagc atctgagtta tcacgttgaa agcctgctgg
     1081 aacaggcagc taaaaaagag ctgaactacc gggagttcct gtgcatggcg ctacagcagg
     1141 aatggaacgg caggcatcag cgcggtatgg agtccaggct gaagcaggct cgtctgccgt
     1201 gggtcaaaac gctggagcag ttcgacttta
//
LOCUS       DAJVRF010000308         1207 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJVRF010000308 DAJVRF010000000
VERSION     DAJVRF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.313"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            52..>1207
                     /locus_tag="OQN13_004123"
     CDS             join(52..301,301..>1207)
                     /locus_tag="OQN13_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS1617308.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRKLRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGCAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVA"
BASE COUNT          343 a          294 c          332 g          238 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt cgcaagaaaa ccagtgggct gtgccatgtc gttctcgccg gacagcaggc
      841 tcaccatgaa agcgctggaa atggcatggg aaacccgtgg taagcccggc ggggtgatgt
      901 tccacagcga tcagggcagt cattatacga gcaggcagtt ccggcagtta ttgtggcgat
      961 accagatcag acagagtatg agccggcgcg gaaactgctg ggataacagc ccaatggaac
     1021 gcttcttcag gagtctgaag aacgaatgga tgccggtggt gggttacgta agcttcagcg
     1081 aggcagctca cgccataacg gactatatcg ttggatatta cagcgcacta agaccgcacg
     1141 aatataacgg tgggttaccc ccaaacgaat cggaaaatcg atactggaaa aactctaact
     1201 cggtggc
//
LOCUS       DAJVRF010000309         1182 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJVRF010000309 DAJVRF010000000
VERSION     DAJVRF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.314"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(401..766)
                     /locus_tag="OQN13_004124"
     CDS             complement(401..766)
                     /locus_tag="OQN13_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCS1617309.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            complement(766..1002)
                     /locus_tag="OQN13_004125"
                     /pseudo
     CDS             complement(766..1002)
                     /locus_tag="OQN13_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207968.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(997..1182)
                     /locus_tag="OQN13_004126"
                     /pseudo
     CDS             complement(997..1182)
                     /locus_tag="OQN13_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998190.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          295 a          323 c          254 g          310 t
ORIGIN      
        1 catagattgg gcctcagatt aatgttaagc gtcttgcagg acgcgtaatg ttaactgggg
       61 cttttctctg tctgccttac agtgacatac ccgaggcaga cagcctcaag cacccgcagc
      121 aatcttaccg acaccgataa gaaaacgcta ttttttattg ccagcaccat ctatccaggc
      181 tagtttatct gcgtcagaac ggcgcaatgc gctcgcctga gatacgttta cttgccatgg
      241 taacaacagg taacggcaat tgctgtttct gttttgtttc cttcaaaaac cccggaccat
      301 caatccgggg tttttgtttg ttaaccccaa cggcaaatcg aatacaccac cagcgccacc
      361 gccatcgcaa ttcctaccgt ggtgaatgcc tcaggccagg tcattgattc acctcctgcg
      421 gtggttccgg tagcggcatc cagtgagttg agctctctac ttcaacgccg tctttgtcca
      481 caaaagccat cttattcccg ccacgagtgg ggcaaaaaac cttatcccag gaaccgggga
      541 atacattccc gagatcatcg agaagaatca catcgcaata ttcagcagga actgcatcac
      601 tacagcttat ccagccagcc ggagctaccg gagagttgcc agccttacgc atggccattt
      661 ccacgatttc aaccatatct cctggtggaa ttttacaaag ttgcccaata catttctgct
      721 gcctggcata ttcgagaatg tgctccagtt tggttcgatt aatcattatt tatctccctt
      781 aagcatggca gcgcggcagg cgttccagcc atcaacataa tcaaacgtat tactatcgtt
      841 tggcccgatt tcatccggca ctatcggctc tggttggata gtgacgttgg caaaggcagc
      901 acgcaaaccg gttttaattt cttcgatttc atcagagcca agcgatgaat ctgacagtgc
      961 gtgatggaat gcgtaagcca tgtcgtcgtt tactgcaacc ggcactgccg gcgctgcgta
     1021 aagcggtgtt atatctgccc gaaaatcaca tgctttatgc agtcgtaccc aacactcaac
     1081 ttccgctttg tcaaaatata tagcagtgaa cgtattagat tcctggtcaa cttgagtaaa
     1141 cgtcaccttc cacgctacag ctacggcttt ccatccatca gg
//
LOCUS       DAJVRF010000310         1177 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJVRF010000310 DAJVRF010000000
VERSION     DAJVRF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.315"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..518
                     /locus_tag="OQN13_004127"
     CDS             <1..518
                     /locus_tag="OQN13_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1617310.1"
                     /translation="FFKDGRLVTTSQAVADYFDKQHKHVLAKIDTLDCSPEFTSANFS
                     ADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEAYIEKFNAMEEELHK
                     RPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLERFKFYLEQHGWIVAR
                     KEQLVERLMRF"
     gene            complement(645..815)
                     /locus_tag="OQN13_004128"
     CDS             complement(645..815)
                     /locus_tag="OQN13_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617311.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(930..1177)
                     /locus_tag="OQN13_004129"
                     /pseudo
     CDS             complement(930..1177)
                     /locus_tag="OQN13_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          324 a          306 c          242 g          305 t
ORIGIN      
        1 tctttttcaa agatggtcgc ctggttacca cctctcaagc tgttgccgac tacttcgata
       61 aacaacataa acacgtttta gctaagattg acacactcga ctgctctccc gaatttacat
      121 cagccaactt ttcggccgat gttcagactg tcgaaattgg caacggcgca gaacgtgaat
      181 cacgatgcta cctgatcaca aaagacggct tcatgttcct cgtaatggga tttactggca
      241 aaaaagcagc cagactaaaa gaagcttaca ttgaaaaatt taacgcaatg gaagaagaac
      301 tccacaaacg tcctcctgca gcacaaaact cccccgcccc aaataatgga tgcgcattac
      361 tgatccactt cgataaacac ggtcaggtcg agttcacaga aaaagtcccc gccgatgcga
      421 tggtatgcac tctggaacgg tttaaatttt atctggagca acatgggtgg atcgttgccc
      481 gtaaagaaca attggtggaa aggttgatgc ggttttaatg aatgcaaaac cccggatgat
      541 caccgaggtt aattttatcg agcgtgattg ccgctaatcg ccatcgctct gattgagcgt
      601 aaatcattca ggttattcag ttcttccttc tgctctcgct gacgtcaata aatcccatca
      661 ttgcgatcaa cctcatcctt aaccattgat gcgagcagtt cttccagttt ctgcgctgac
      721 agctttacct ggtgatcttc agcatcttcc cgggctattg tggttcgcgc agtggctgat
      781 tttgctgaca tcaccacagg gggcagacgg accattgaac catggcctcc ggaacaaata
      841 agggcaaaaa taacaaccac tatcgaaagc tcacaaacta accgcagcac gttcctgcat
      901 acgacgtgtc tgcggcataa tcccaatgat tactccctga caggatttgc aggccactca
      961 atatcaggtg cagttgatgt atcaacacga ttcaacaata cccgatattt attccatgcc
     1021 tccagcaacg atctttcttc ctccgttgcg atttccagat ctacagcatc ctgcagtggc
     1081 gcaatatact cactaaattc ctggatgtag aactgtgtgg tgacggtctt ccagccattc
     1141 ggctcctgct gtatcgaagc atactaggct atttcaa
//
LOCUS       DAJVRF010000311         1160 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJVRF010000311 DAJVRF010000000
VERSION     DAJVRF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.316"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..478)
                     /gene="umuC"
                     /locus_tag="OQN13_004130"
     CDS             complement(<1..478)
                     /gene="umuC"
                     /locus_tag="OQN13_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCS1617312.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQ"
     gene            complement(478..897)
                     /locus_tag="OQN13_004131"
     CDS             complement(478..897)
                     /locus_tag="OQN13_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCS1617313.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT          334 a          294 c          240 g          292 t
ORIGIN      
        1 tctgccgctg ccattttttt gccgcatgat tggcaagctt agccagcgtt ttggtctggg
       61 cgatccccat accaacagta agatgggtac gttgtagcac cgttgcgcga atttctctgc
      121 caaaatcagt cagatcgcga caattacgca cacctgtcag atcgcagaat gcctcatcaa
      181 tactgtaaat ctcgacgcgg ggcgatagtt cttccagcgt cgacatcacc cgattgctca
      241 tgtctgcgta aagctcataa ttgctgctaa agcaaaccac accacagcga cgaaacagat
      301 ctttttgttt gaaccaggga tcgcccattt taacacccag cgcctttgcc tcagcgtttc
      361 gggcgataac gcaaccgtca ttattcgata gcacaaccac cggtttaccc cataaatcag
      421 ggcgaaacac cgtctcacag ctggcataaa acgcgtttac atcacagaga gcaaacatca
      481 gcgcatcgcc ttaacgacgt ggatcaccac accaaagaca tccagcgtat cttcgctact
      541 gatggtaatg ggcgagtacg cactgttcat gggaataagc tgtaccgtcg ggcgtagttg
      601 caattttttc accgtaaact cgccgtcaac agcagcgatg acaatatcac catggctggc
      661 ggtaatagcg ctatcgacaa tcagtaaatc accgtcacta attccaccat caatcataga
      721 atcaccactt gctttgacga agtaagtcgc gctgggatgc tggatcaaca gttgattcag
      781 atcgatgcgc tgttcgacgt aatctgctgc cggtgaagga aagccacact gaacaagatc
      841 gctaaatagc ggaaaagtca caatttcgcg gagatccgca ggcttgataa acaacataat
      901 aatctgcctg aagttatact gtttttatat acagtaatct gttcttgcca gcagatcaat
      961 actgattcag gctatcaata tttgtcgctg catagactgc tgatttttcg ttctcttatc
     1021 ctgtgctcac gtggccttct ggcgacgacg ctcatccagc agaaatgaaa aatatcaccc
     1081 ggcaaaaaaa tagaatagaa gcattcgcca taatgacatt aaacattgtt tgatatttat
     1141 catattaata gaaataaaga
//
LOCUS       DAJVRF010000312         1153 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJVRF010000312 DAJVRF010000000
VERSION     DAJVRF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.317"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(192..794)
                     /locus_tag="OQN13_004132"
     CDS             complement(192..794)
                     /locus_tag="OQN13_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCS1617314.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(731..>1153)
                     /locus_tag="OQN13_004133"
     CDS             complement(731..>1153)
                     /locus_tag="OQN13_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCS1617315.1"
                     /translation="HLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQN
                     LYLDNMEVTGFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGGLLHHIPEQLSKRE
                     RYTDKWQRRTHSLWRHRAWRASAFTGIYNDLVAASTFV"
BASE COUNT          266 a          379 c          266 g          242 t
ORIGIN      
        1 ctttttaatc catccaccgg accgtcaacc ggcccttcgc tgatggcatc aatcacactc
       61 agcaactgcg tggatttcag gttgtccttc gcttcgcgcg gggtatgccc cttactgctg
      121 cctttaccca ttcctcacgc tccataaacg acaaaaccgc ccgcaggcgg tttcacataa
      181 aacattttgc atcagcgacc aatcaccaca acctgaccac catccccttc gtctgccgtg
      241 ctgatctcct gagaaaccac acgtgacccc acgcgcattt caccgtacag aacgggcaga
      301 acattgccct gggcaaccat gttatccagt gacgagaaat acgtgttctg cttaccgtta
      361 tccgttgtct gtgtacgggg agttctggct ttcggtgcca gcatctgtgc aacaccgcca
      421 agcgtcatac tggcaccgag agaaaacagc agattactcg ccataattcc tacccccggc
      481 atccatatag caaccgccat aacagccgcc cccagcaccg cctgaaacac accgccactt
      541 ttagctccca ccagacgcgg tactatgtgg atcactgcac catttgccag cggctcatta
      601 agacgggcag acaattcggt ttcacctgca tcacgcccgg caatgcgcac ctgataccag
      661 ccgtcactca gtttctgacg aaacgccggg agctgtgtgg ccagtgcccg gatggcttca
      721 gcccccgttt tcacacgaag gtcgatgcgg cgaccaaatc gttgtaaatc cccgtaaagg
      781 cagatgcgcg ccatgcccgg tgacgccaga gggagtgtgt gcgtcgctgc catttgtcgg
      841 tgtacctctc tcgtttgctc agttgttcag gaatatggtg cagcagcccg ccgtcaccgc
      901 agtaaatggc ggcatgattc ggcaccgatg aaccaaaaca gcacagcagc acatcgcccg
      961 gctgtgcctc tgtcagtgcg acacggtaaa aacccgtcac ctccatattg tcaagataga
     1021 gattctgacc gttacgccac cagtcatccc cgcgatgaaa atccggcatc tcaatccctg
     1081 ccagatgata agcatcccgg aacagcgtgt aacagtccgt caccccgtgc tcaaagcgcc
     1141 gcccggtgag atg
//
LOCUS       DAJVRF010000313         1144 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJVRF010000313 DAJVRF010000000
VERSION     DAJVRF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.318"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            743..>1144
                     /gene="uspC"
                     /locus_tag="OQN13_004134"
     CDS             743..>1144
                     /gene="uspC"
                     /locus_tag="OQN13_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCS1617316.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKFIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDV"
BASE COUNT          371 a          220 c          220 g          333 t
ORIGIN      
        1 gtttcaccgc accccgtgat gtcgccggga agccccggta aaaaataatt agcattagaa
       61 tagttgcgat aagctgcaat aagcagaacc acctttttgg tttaatatgt ccttacaaat
      121 agaaatgggt ctttacactt atctaagatt tttcctaaat cgacgcaact gtactcgtca
      181 ctacacgcac atacaacgga ggggggctgc gattttcaat aatgtgtgat gcagatcaca
      241 caaaacactc aattacttaa cataaatgta taagtcatac ttttgttttg ggtgtatttc
      301 aatatgttaa aaagtttttc gctacgctag caagcaaaaa tgaaacagga ataatcgaaa
      361 tgggatgttg cgcacagtta aaataaatca ccgtaaataa tcatctgcta taaataatca
      421 ctttcatgca ataccagata aactattttt aaacagacac ttaccacaca acaaactaat
      481 taaatacaaa gaaaggcaac aatcaactta atgttaatga aatgttttac atacattaac
      541 cacttgttta atgcggagaa tcagtacact attttcttat aattacattt gaaattatat
      601 gatcaccggg agtgattgat taattgtctt tactaataat cagactaata tttacctgtt
      661 tgaccgagtt gggattgcgt cgtttctcca ttaggagtaa agctttaatg tcacctgaag
      721 ttcacagaat aaggaacagg ttatgagcta tagcaatatt cttgtcgctg ttgcggttac
      781 accggaaagt cagcaactgc tggcaaaagc ggtatctatc gccaggccag taaagggaca
      841 catcagttta attactctcg cttccgaccc ggaaatgtac aatcaattag ccgcgccgat
      901 gcttgaagat ttacgcagcg tgatgcaaga agaaacgcaa agctttcttg ataagttcat
      961 tcaggatgcg gggtatcccg ttgacaagac gtttattgcc tacggtgagt taagcgaaca
     1021 tattctggaa gtatgtcaca aacatcattt cgatttggtg atttgcggta atcacaatca
     1081 cagtttcttt tcgcgagcat cctgctcggc gaaaagagtg attgcctcaa gtgaggtcga
     1141 tgtg
//
LOCUS       DAJVRF010000314         1137 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJVRF010000314 DAJVRF010000000
VERSION     DAJVRF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.319"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..989
                     /locus_tag="OQN13_004135"
     CDS             <1..989
                     /locus_tag="OQN13_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HCS1617317.1"
                     /translation="LITGDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTA
                     DGVTGMGSSEVTIASPGTLDILASTNSAGDYTLTNALKGDGLMRVQLSSYDKMFGFTH
                     ATGTEFAGVAQLKDRTFTLERDNTAALTHAMLQSDSENTTSVNVGEQSIGGLAMNGGT
                     LIFDTDIPAATLAEGYISVDTLVVGAGDYTWKGRNYQVNGTGDVLIDVPKPWNDPIAN
                     NPLTTLNLLEHDDSHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVV
                     AEGDYGFRLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAK"
     gene            988..1137
                     /locus_tag="OQN13_004136"
                     /pseudo
     CDS             988..1137
                     /locus_tag="OQN13_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          291 a          275 c          331 g          240 t
ORIGIN      
        1 tgctgattac aggtgacgat aaagacggaa aagtgatcca tgatgcaggc catacggtgt
       61 tcaatgcagg gaatacttat agcggtaaaa cgctggtcaa tgacggcctc ctgaccattg
      121 cgtctcatac ggcagatggg gtaacgggca tgggttcgag tgaagtcacc attgcaagcc
      181 ccggtacgct cgacattctc gcatcaacga acagtgcagg agattacacg ctgaccaatg
      241 cgctcaaagg cgatggcttg atgcgagtgc agctgtcatc ctacgacaag atgtttggct
      301 ttacccatgc aacagggact gaattcgccg gtgttgccca actgaaagac agaaccttca
      361 ctctggaacg cgacaacacc gctgcgctta ctcacgcgat gttgcagtct gacagtgaaa
      421 ataccacatc ggtaaacgtg ggagagcaat ccattggtgg actggccatg aatggcggta
      481 cgctcatttt cgatacggat attcctgctg cgacgcttgc agagggatat atcagcgtcg
      541 atacgctggt tgtcggcgcg ggtgactaca cctggaaagg ccgtaactat caggtaaacg
      601 ggacgggcga cgtgcttatc gacgtgccta aaccgtggaa tgatcccata gcgaataacc
      661 ctctgacgac gctcaatttg ctggaacacg atgatagcca tgtcggcgtt caactggtga
      721 aggcgcaaac ggttattggg tcgggtggct cattaacgtt acgtgattta cagggcgacg
      781 aggtggaagc ggacaaaacg ttacacattg cgcaaaacgg aacggtggtc gccgagggtg
      841 attatggatt ccgcctcacg accgcaccag gtgatggttt gtacgttaac tatgggctga
      901 aagcgctgaa catccatggt gggcaaaagc tgacgttagc cgaacatggc ggagcctatg
      961 gcgcaacggc cgatatgtcg gcaaaatagc catcgcttac acgaccgtct gggtgagccg
     1021 cagtatacag attcactgca ttctcaggga tcggcaagca gtatgtggat gcgtcatgtc
     1081 ggggggcacg aacgtttcag gaccggtgac ggccagctaa atactcaggc taaccgc
//
LOCUS       DAJVRF010000315         1117 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJVRF010000315 DAJVRF010000000
VERSION     DAJVRF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.320"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            84..1115
                     /locus_tag="OQN13_004137"
     CDS             84..1115
                     /locus_tag="OQN13_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HCS1617318.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          301 g          228 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgttcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagagcatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag aatgaaaaat
      721 attatctggc cggagcgctg cacagcggga caggtaaagt cagctgtgtg ggcggcaaca
      781 gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca taccgtcggg
      841 cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg gaaacacaga
      901 gctggctgaa ggagaacccg aagttcaggg tcatttatca gccggtttac tcgccatggg
      961 tgaatcatgt tgaacggcta tggcaggcac ttcacgacac aataacgcgt aatcatcagt
     1021 gcagctcaat gtggcaactg ttgaaaaaag ttcgccattt tatggaaacc gtcagcccat
     1081 tccccggagg caaacatggg ctggcaaaag tgtagcg
//
LOCUS       DAJVRF010000316         1111 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJVRF010000316 DAJVRF010000000
VERSION     DAJVRF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.321"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..120)
                     /locus_tag="OQN13_004138"
     CDS             complement(<1..120)
                     /locus_tag="OQN13_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCS1617319.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHN"
     gene            complement(132..824)
                     /gene="rutB"
                     /locus_tag="OQN13_004139"
     CDS             complement(132..824)
                     /gene="rutB"
                     /locus_tag="OQN13_004139"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCS1617320.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(824..>1111)
                     /locus_tag="OQN13_004140"
     CDS             complement(824..>1111)
                     /locus_tag="OQN13_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCS1617321.1"
                     /translation="SWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVA
                     RMLDEVASVPGAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          256 a          305 c          311 g          239 t
ORIGIN      
        1 attatgttga tcaaaagcca gcgtaccgga gacatacacc acgccatcag ccagcgtgcc
       61 gggaacgaag ggggccagcg gtgcgctgct gccagcggga ataattacgg attttggcat
      121 cgttaaactc cttaagcgat acgagcaaag gacgtgggag aaagcgcgtc gcagaacgtt
      181 tcgacgtcgc tgacccagcc aaaaaaggtt tcgatattga acaacgcggc tttctgtgca
      241 aattcaggcc ccgcctggtg tgttgcgtct tccagcacca cgccgaaata ctccagaaaa
      301 aagccatcgc gtagcgtcga ttcgacgcag acgttggtag cgataccggt gaaaaccaga
      361 tggcgtattc cacggctgcg caaaatgctg tccagcggcg tattgaagaa accgctgtag
      421 cgcggcttcg gcagcacaat atcgccaggc tgcggcacca gttcatccac cagttgataa
      481 tcccaggagc ctttcgccag caatttcccc tgcagctgcg gctgcttacg catggttttc
      541 agggcgttcg atttatgaaa attcggtgag ccgggtccgc cagcttcgac atactgttca
      601 tcccagccat tttgaaacca gatgatcagc atccctgccg ctcgcgctgc ggtcacggcg
      661 gtttgaatgt tggcaatgac cgggcgagtg gttgagacat caaacccggc gagatctaag
      721 tagccgcctg gcgtggcata agcgttttgc atatccacca cgatcagcgc actttgctgc
      781 ggatcgaagg taatggcttc cggtcgagcg gttaaggtcg tcatcatgcc acctcctgag
      841 tcagcgcagg gagatgggcg cggcactgca tcagtggttg aatgcgctcg ccgaaggttt
      901 cgattcccga cagaaaatca tcgaaggtca acagcacgcc ttcggcacca ggcacgcttg
      961 cgacttcatc taacatgcgc gcgacactgg cgtaagaacc gactaatgtc cccatattga
     1021 tgtttaccgc cgaagtggga tcggccatct gacggacgtt ggtgtcagta ccggagcggg
     1081 tatctttctg actttgttcg gttagccagc t
//
LOCUS       DAJVRF010000317         1105 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJVRF010000317 DAJVRF010000000
VERSION     DAJVRF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.322"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..>1105)
                     /locus_tag="OQN13_004141"
     CDS             complement(<1..>1105)
                     /locus_tag="OQN13_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1617322.1"
                     /translation="TDLALPCPLPFILFRAYSSYRTRTPAPVGVFGPGWKAPFDIRLQ
                     IRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSALWQVLP
                     EDVRLSPHVYLATNSLQGPWWILSWPEPPAYRVLTVVVDGFGRSLTFHRAAEGDVAGA
                     VTGVTDGAGRRFHMALSTQAQRAEASRKQRASSLSSPASPRSVSSSQVFPDTLPAGTE
                     YGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGELRAVYDRSGTQVRGFTYDA
                     EHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRFEYGESRVIITDSLNRREV
                     LYTEGEGGLKRVVKKEHADGSITRSEYDEAGRL"
BASE COUNT          190 a          398 c          322 g          195 t
ORIGIN      
        1 ttcagcctcc ccgcctcatc atactcgctg cgggtgatgc tcccgtcggc atgctccttc
       61 ttcaccaccc gcttcaggcc accctcgcct tccgtgtaca gcacctcccg ccggttcagg
      121 ctgtccgtga tgattacccg gctctctccg tactcaaagc ggtagtccag cccctccggg
      181 ttgacctgct ccgtcacccg accggtatca tcataccggt agcggctctc cggcctcccc
      241 gcataatggt gcgccaccat ccgcccggcg tgctccgcat cataagtaaa cccgcgaacc
      301 tgcgtcccgc tgcggtcata caccgcccgc agttcaccac cggccgtgta cgtgtagcgc
      361 gccagcggcg cagtcggctg ttcatccggg tatgccgggt cgtgtgtcag ccataccgcc
      421 tccagccgga taccgttatc ggcaccgtac tctgtaccgg cgggcagcgt gtcggggaaa
      481 acctgagaag aggaaacaga acggggactg gccggggaag ataaggaaga ggcgcgctgt
      541 ttacgggacg cttccgcccg ctgcgcctgc gtgctcagcg ccatgtgaaa acggcggccc
      601 gcgccgtccg tcacccccgt caccgcgccc gccacgtcac cctccgccgc ccggtggaaa
      661 gtcagtgagc gcccgaagcc atccaccacg accgtcagta cccggtatgc gggcggctcc
      721 ggccagctga gtatccacca cggcccctga aggctgtttg tcgccaggta cacatgcgga
      781 ctcaggcgaa catcttccgg cagcacctgc cagagcgcgc ttagcggctg cgaactgtgc
      841 tgcgccgcca ctccgccccg cgccagccag agtgactcgc tgcggctgta gcttatctcg
      901 ccgggaaaca gcggctcaaa gtgaatgctc ctgccgccgc tgtcgttgag tatcaggcct
      961 tcatcgcgta tctgtaagcg gatatcgaag ggcgctttcc agccgggacc aaacaccccc
     1021 accggcgcgg gtgttctggt ccggtagctg ctgtaggcgc ggaaaagaat aaacggcagc
     1081 gggcagggca gcgcaaggtc cgtct
//
LOCUS       DAJVRF010000318         1088 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJVRF010000318 DAJVRF010000000
VERSION     DAJVRF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.323"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            212..>1088
                     /gene="nadA"
                     /locus_tag="OQN13_004142"
     CDS             212..>1088
                     /gene="nadA"
                     /locus_tag="OQN13_004142"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCS1617323.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT          294 a          259 c          265 g          270 t
ORIGIN      
        1 caggcatcct caatttcact ttgtaaacct gatgacatcg tcagagctta ctgtgcaagc
       61 aactctacgt cggtggaatt aggcgtaatt cgagtgactt ttccccacca ttcgactatc
      121 ttgtttagca tataaaacaa attacgccga tagtagcgaa tattacgcta atgtcggttt
      181 taacgttaag cctgtaaaac gagatggtaa gatgagcgtg atgtttgatc cagacacggc
      241 gatttatcct ttccccccga agccgacgcc gttaagcatt gatgaaaaag cgtattaccg
      301 cgagaagata aaacgtctgc taaaagaacg taatgcggtg atggttgccc actactatac
      361 cgaccccgaa attcaacaac tggcagaaga aaccggtggc tgtatttctg attctctgga
      421 aatggcgcgc ttcggtgcaa agcatcccgc ttctacgttg ttagtcgctg gggtgagatt
      481 tatgggagaa accgccaaaa ttctcagtcc ggaaaaaaca attctgatgc tgacacttca
      541 ggctgaatgt tcactggatc tcggctgccc tgttgaagaa tttaacgcat tttgcgatgc
      601 ccatcccgat catactgtcg tcgtctacgc caacacttct gctgcggtaa aagcgcgcgc
      661 agattgggtg gtaacttcaa gcattgccgt cgaacttatt gatcatctgg atagttttgg
      721 tgaaaaaatc atctgggcac ccgacaaaca tctggggcgt tacgtgcaaa aacagacggg
      781 cgcagacatt ttatgctggc agggtgcctg tattgtgcat gatgaattta agactcaggc
      841 gttaacccgc ttgcaagaag aatacccgga tgctgccata ctggtgcatc cagaatcacc
      901 acaagctatt gtcgatatgg cggatgcggt cggttccacc agtcaactga tcgctgctgc
      961 gaaaacattg ccacatcagc ggcttatcgt ggcaaccgat cggggcattt tctacaaaat
     1021 gcagcaggcg gtgccagata aagagttact ggaagcacca accgcaggtg agggtgcaac
     1081 ctgccgca
//
LOCUS       DAJVRF010000319         1080 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJVRF010000319 DAJVRF010000000
VERSION     DAJVRF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.324"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..185)
                     /locus_tag="OQN13_004143"
     CDS             complement(<1..185)
                     /locus_tag="OQN13_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1617324.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFS"
     gene            complement(233..805)
                     /locus_tag="OQN13_004144"
     CDS             complement(233..805)
                     /locus_tag="OQN13_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HCS1617325.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          352 a          226 c          197 g          305 t
ORIGIN      
        1 ttgctaaata cagaaataga tttatcagca ttattactta agtaaaccac ccgcgtacca
       61 ccaatgacaa cgcctgcata agttaatgct ggttggccta aaaaaacgct gagtataatc
      121 gcggtaaata acttacatga aactgttgtt ttaaagagcc tgattaaaca actttttctg
      181 gacataacga ctccctggcc aaagccaact gaataaaaag gcccttgata aattatttct
      241 tatattcgac agtaaattgt gaatcagcgt tcgccgtacc agccgttacg gtcgcattgg
      301 ttgccacata gcgtgcaata aacttcattt cagccttgcc ttcagcatcg atagcggtat
      361 aagcagagtc gttataaagt ttaacctgcg aattatcgga ctggttaaaa atacgaatac
      421 caacgccggt tgccgcaatc gcttgtcctg taccggtaaa atcaccaggg ttgcctgctg
      481 taagcggcgt acccactttc aggtcgccgt cgccgccagg cgcttctgta ccatcaaaac
      541 gaacggctgc ttgagtgtac gttgccgggc aattttcaag tttaatactg aacttctgtg
      601 gcgacgccag actacctaca cctgaaaatg catttttact gactttaccc agtgggacaa
      661 tttgatcggc tgtatcggag gtaacttcac aagtagtgtc gacaatttca ccgacgaagt
      721 gaacaacacc atcttctgcc agtgcatgcc cggccagcat tagagatgcc agagtgaatg
      781 caccgcctat aatattacgc ttcataaaaa ctccattgat ataatatatc ctttaccatg
      841 aatcatcact tatattaata agtgtatttt tgattcaaag gaatagtaca ccaaagagac
      901 ggctttatct aaaaataata ttaattacgc aaaaataaaa tataaattca ttgtttaaat
      961 attatttaaa cagtaaaaaa tcaagtttgt tatcagacat taatgataat tcagaatata
     1021 tttatataga aagatagata aatttattaa tgtcagttat aaaaaagggc caccggtcgg
//
LOCUS       DAJVRF010000320         1045 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJVRF010000320 DAJVRF010000000
VERSION     DAJVRF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.325"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            76..342
                     /gene="hipB"
                     /locus_tag="OQN13_004145"
     CDS             76..342
                     /gene="hipB"
                     /locus_tag="OQN13_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HCS1617326.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            342..>1045
                     /gene="hipA"
                     /locus_tag="OQN13_004146"
     CDS             342..>1045
                     /gene="hipA"
                     /locus_tag="OQN13_004146"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HCS1617327.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVER"
BASE COUNT          313 a          236 c          260 g          236 t
ORIGIN      
        1 attgacttta tcctcactaa aggataaaac tgataatatc cccttaagcg gataaatttg
       61 ctgtggacgt atgacatgat gagctttcag aagatctata gcccaacgca attggcgaat
      121 gcaatgaaac tggttcgcca gcaaaatggc tggacgcaga gcgagctggc gaaaaaaatt
      181 ggcattaagc aggcgacgat ttccaatttc gaaaacaacc ctgacaatac cacgctgacg
      241 acctttttta agattttaca gtcgcttgaa ctctcaatga cgctatgcga cgcgaaaaat
      301 gcctcgccag aagcaacaga acagcaagat ctggagtggt aatgcctaaa cttgtcactt
      361 ggatgaacaa ccagcgggta ggcgagttaa cgaagttagc caacggcgcg cacaccttta
      421 agtatgcacc ggagtggtta gccagccgtt atgccagacc gttgtcactt tcgctgccat
      481 tgcagagggg gaatatcacc tctgatgccg tatttaactt cttcgataac ctgttacccg
      541 atagcccgat tgtacgtgac cggatcgtta aacgttatca tgccaaatcc agacaaccgt
      601 ttgatttgtt gtcagaaata gggcgagaca gcgttggtgc cgtgacgtta atacccgaag
      661 acgaaaccgt aacgcgtccg ataatggcat gggaaaagct tactgaagcc agacttgaag
      721 aagtattaac ggcttataaa gcagatatcc cgctaggcat gattagagaa gaaaatgact
      781 ttcgcatctc ggttgctggc gcacaggaga agacagcact gctcagaata ggcaatgact
      841 ggtgcattcc gaaaggaata acgccgacga cgcacatcat taaattaccg attggcgaaa
      901 tcaggcagcc caatgcgacg ctcgatctca gccaaagcgt tgataatgag tattactgtc
      961 tgctgctggc gaaagaactt gggttgaatg ttccggacgc agaaatcatt aaagcgggaa
     1021 gggtgcgcgc gttagcggtc gaacg
//
LOCUS       DAJVRF010000321         1011 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJVRF010000321 DAJVRF010000000
VERSION     DAJVRF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.326"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            11..>1011
                     /locus_tag="OQN13_004147"
     CDS             11..>1011
                     /locus_tag="OQN13_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCS1617328.1"
                     /translation="MSAYLRYIVQKADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPE
                     QRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEG
                     NRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRK
                     DIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTH
                     DRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFIT
                     SSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQ"
BASE COUNT          261 a          235 c          238 g          277 t
ORIGIN      
        1 cgacagagca atgagtgcct atctgcgcta tatcgtgcag aaagcagact cctcctttct
       61 ttatgataag tatcagaatc agagtattgc cgcgcatgtg atgcgcgctc tcgctgctga
      121 gcagtcggaa gtgtcgccag aacagcggcg cgccatctgc gaggcttttg agtctgccaa
      181 taatacccat ggcttaaacc tgactgccca taaatacccg ggcttacgcg gcacactaca
      241 aaccgcatcc actgactgcg acacaattgt ggaagctgca gcactattac ccgcttttga
      301 tcaggcagtg gaaggcaacc gccaccagga tgattacggt tcaggtcttg ggatggccga
      361 agagaaattt cactattatc tcgatctcaa tgaccgctat gtctattttt atgagccggt
      421 taatgttgaa tactttgcga tgaataactg gtccttcctg cagtcaggaa gtattggcat
      481 cgatcgcaaa gatattgaaa aggtatttac cgggcgtacc gtattgtcga gcatttacca
      541 ggatcagcgt actaaacaga acgtgatgag tttgctgacg ccggtatatg tcgcagggca
      601 gctaaaaggg attgtgctgc tggatattaa caaaaacaat ttgcggaata tcttttacac
      661 tcatgaccgc cctctcctct ggcgttttct caatgtcacg ctaaccgata ccgattcggg
      721 gcgcgacatt atcatcaacc agagcgaaga taatctgttc cagtatgtca gttacgtcca
      781 tgacttaccg ggcggcattc gtgtctcgtt atccattgat attctttact ttatcacgtc
      841 ttcgtggaaa agcgttctgt tctggatttt gacggcgtta attttgctga atatggtccg
      901 aatgcacttc cgtttatacc aaaatgtgtc gcgagaaaat attagtgatg cgatgactgg
      961 actgtataat cgcaaaattt taacccctga actggagcag cggttgcaga a
//
LOCUS       DAJVRF010000322         1010 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJVRF010000322 DAJVRF010000000
VERSION     DAJVRF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.327"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..510
                     /gene="lpxL"
                     /locus_tag="OQN13_004148"
     CDS             <1..510
                     /gene="lpxL"
                     /locus_tag="OQN13_004148"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCS1617329.1"
                     /translation="LGARQFGIQEPGIGVYRPNDNPLIDWLQTWGRLRSNKSMLDRKD
                     LKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQAATTTGTWMLARMSGACLVPF
                     VPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKVVEKCIMMAPEQYMWLHRRFKT
                     RPEGVPSRY"
     gene            682..879
                     /locus_tag="OQN13_004149"
     CDS             682..879
                     /locus_tag="OQN13_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCS1617330.1"
                     /translation="MSPCGNDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIIPS"
BASE COUNT          247 a          267 c          252 g          244 t
ORIGIN      
        1 ctgggcgcgc ggcagtttgg tattcaggaa ccggggattg gcgtttaccg cccgaacgat
       61 aatccactga ttgactggct acaaacctgg ggccgtttgc gctcaaataa atcgatgctc
      121 gaccgcaaag atttaaaagg catgattaaa gccctgaaaa aaggcgaagt ggtctggtac
      181 gcaccggatc atgattacgg cccgcgctca agcgttttcg tcccgttatt tgccgttgag
      241 caggctgcga ccacgaccgg aacctggatg ctggcacgga tgtccggcgc atgtctggtg
      301 cccttcgttc cacgccgtaa gccagatggc aaagggtatc aattgattat gctgccgcca
      361 gagtgttctc cgccactgga tgatgccgaa actaccgccg cgtggatgaa caaagtggtc
      421 gaaaaatgca tcatgatggc accagagcag tatatgtggt tacaccgtcg ctttaaaaca
      481 cgcccggaag gcgttccttc acgctattaa atctcccctg ccggatgctt cagaatggca
      541 tccggcatta ccacagcaaa tccccctgat ttagcgataa aagctctctg gattgcgccc
      601 cctggaagtc gggcgcataa ttagtgtgct tatcttttct tcttatgttc accgcgcctg
      661 gcgcaccaac agcggattgc tatgtcaccc tgtggaaatg acacccctat aaactggaaa
      721 cgaaacctga tcgtcgcctg gctaggctgt tttcttaccg gtgccgcctt cagtctggta
      781 atgcccttct tacccctcta cgttgagcag cttggcgtta ccggtcactc cgccctgaat
      841 atgtggtccg gtattgtctt cagtataata ccttcctaaa tacactacct gagagaagag
      901 atgaaaagag ctatcagcta catgaggttt tcaactgaga agcagataaa aggggactct
      961 atacgaagac agtctaagct ggtcactgac tggttatcaa agaaccctga
//
LOCUS       DAJVRF010000323         1003 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJVRF010000323 DAJVRF010000000
VERSION     DAJVRF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.328"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>1003
                     /locus_tag="OQN13_004150"
     CDS             <1..>1003
                     /locus_tag="OQN13_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HCS1617331.1"
                     /translation="SGNLIEGLTVYFALKSGSATLTSLTAVTDQNGIATTSVKGAMTG
                     SVTVSAVTTAGGMQTVDITLVAGPADASKSVLKNNRSSLKGDFTDSAELHLVLHDISG
                     NPIKVSEGLEFVQSGTNVPYVQVSAIDYSKNFSGEYKATVTGGGEGITTLIPVLNGVH
                     QAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGAYYQLNNDNFAPGKTAA
                     DYEFSSSASWVDVDATGKVTFKNVGSNSERITATPKSGGPSYVYEIRVKSWWVNAGEA
                     FMIYSLAENFCSSNGYTLPRANYLNHSSSRGIGSLYSEWGDMGHYTTEAGFQSNMYWS
                     "
BASE COUNT          294 a          222 c          253 g          234 t
ORIGIN      
        1 cagtggtaac ctgatcgaag gtctcactgt gtactttgcc ttaaaaagcg gctctgccac
       61 attaacgtca ttaacagcgg tgaccgatca aaacggaatc gcgacaacaa gcgtgaaagg
      121 agcgatgaca ggtagcgtca cggtaagcgc agtcacgacc gctggtggaa tgcaaacagt
      181 agatataacg ctggtggcag gcccagcaga cgcctcaaag tcagtcctta agaacaatcg
      241 gtcatcattg aaaggagact ttaccgatag tgctgagcta catcttgttc tgcacgatat
      301 atcaggcaat ccgatcaaag tttctgaagg gctggaattt gtacagtcag gtaccaacgt
      361 gccctatgtg caagttagtg caattgacta cagtaaaaat ttctcaggcg agtacaaagc
      421 cactgttaca ggaggcggag agggtatcac aacgctgatc cctgtattga atggtgttca
      481 tcaagctggt ctgagtacca caatacaatt cactcgcgca gaagacaaaa taatgagcgg
      541 tacagtatca gtcaatggta ctgacctacc gacaactaca ttcccttcgc aggggttcac
      601 cggggcgtat tatcagttga ataatgacaa ctttgcccca ggaaaaacgg cggctgatta
      661 tgagttttca agctctgcct cctgggtcga tgttgatgct accggtaaag tgacatttaa
      721 aaatgtcggc agcaattcgg aaaggattac ggcgacacca aaatcaggag gccctagcta
      781 tgtatacgaa atccgtgtga agagttggtg ggtgaacgcc ggcgaggctt tcatgatata
      841 cagccttgct gaaaattttt gcagcagcaa tggctacacg ctccccagag caaactattt
      901 aaaccacagt agttcccgag gcatcgggtc actgtacagt gaatggggag atatggggca
      961 ttacacgact gaagctggtt ttcaatcaaa tatgtattgg tca
//
LOCUS       DAJVRF010000324         1003 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJVRF010000324 DAJVRF010000000
VERSION     DAJVRF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.329"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            29..352
                     /locus_tag="OQN13_004151"
     CDS             29..352
                     /locus_tag="OQN13_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCS1617332.1"
                     /translation="MVNFKDKNMPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKY
                     LKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEE
                     LKALV"
     gene            complement(472..651)
                     /locus_tag="OQN13_004152"
     CDS             complement(472..651)
                     /locus_tag="OQN13_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCS1617333.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            725..>1003
                     /gene="pabB"
                     /locus_tag="OQN13_004153"
     CDS             725..>1003
                     /gene="pabB"
                     /locus_tag="OQN13_004153"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="para-aminobenzoate synthase component 1; catalyzes
                     the formation of 4-amino-4-deoxychorismate from chorismate
                     and L-glutamine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HCS1617334.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIR"
BASE COUNT          276 a          232 c          229 g          266 t
ORIGIN      
        1 taaggtcata tatagcctta tcgataacat ggttaatttt aaggataaga atatgcctgc
       61 tgtaatagat aaagccctgg atttcattgg tgccatggat gtatcagcgc caacaccaag
      121 ttcgatgaat gaaagcacgg cgaagggaat ctttaaatat ttaaaagaac tgggagtacc
      181 tgcgagtgcc gctgatatta cggcgcgagc tgaccaggaa ggctggaacc ctgggtttac
      241 ggaaaaaatg gttggatggg caaaaaaaat ggagtcaggt gaacgctctg tgattaaaaa
      301 tcctgaatac ttttcaacat atatgcagga agaactaaaa gcactggtct gagtaaaatt
      361 tacatcagca taaatggatc aggacgcttt taatcacata taaaaagcgt cctttttctc
      421 acaatcaaat aatcatcggc ttgtataacg aaatcaatac ggattagtgt gttactcgtc
      481 ttcatcctca aaacgcgcca cgattcgctc gccggaatgg ttggcacgca gctcttctgc
      541 caccaacgcg attgcctggc cgctgctcat cccttgtgcc atcagttcct ggatccgctc
      601 gacagctttt tgctgctgtt catgggtgag tgaaggtaaa cctgcaaaca ttgttaactc
      661 ctgctaaatt gttggcgcta attatttcat gctacccggc acatagccag tagagtcagg
      721 actgatgaag acgttatctc ccgctgtgat tactttaccc tggcgtcagg acgccgctga
      781 attttatttc tcccgcttaa gccacctgcc gtgggcgatg cttttacact ccggctatgc
      841 cgatcatccg tatagccgct ttgatattgt ggtcgccgat ccgatttgca ctttaaccac
      901 tttaggtaaa gaaaccgttg ttagtgaaag cgaaaaacgc acaacgacca ctgatgaccc
      961 gctacaggtg ctccagcagg tgctggatcg cgcagacatt cgc
//
LOCUS       DAJVRF010000325          996 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJVRF010000325 DAJVRF010000000
VERSION     DAJVRF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.330"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            27..665
                     /gene="ytfB"
                     /locus_tag="OQN13_004154"
     CDS             27..665
                     /gene="ytfB"
                     /locus_tag="OQN13_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HCS1617335.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(649..>996)
                     /locus_tag="OQN13_004155"
     CDS             complement(649..>996)
                     /locus_tag="OQN13_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCS1617336.1"
                     /translation="NPWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGK
                     KHNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETIL
                     SWLLVDPSSTAHE"
BASE COUNT          269 a          252 c          250 g          225 t
ORIGIN      
        1 aacaagacgc ggagcaggag gaaaccatgc ccgggcgctt tgaactaaaa ccaaccctgg
       61 agaaagtctg gcacgcgccg gacaattttc gcttcatgga cccgctgccg ccgatgcatc
      121 gtcgggggat cattattgcc gccattgtat tggtggtcgg atttctgctt ccatctgatg
      181 atacgcccaa cgcgccggtg gtgacgcgtg aagcgcagtt agatattcag tcgcaatcac
      241 aaccgccgac agaagaacag ctgcgggcgc aattagtcac accacaaaat gatcctgatc
      301 aggtggctcc ggtcgctcca gaacctatac aggaaggcca gccagaagaa cagccgcaaa
      361 ctacccaaac gcaaccattc cagccagata gcggaattga taatcaatgg cgctcttacc
      421 gtgtcgaacc cggtaaaacg atggcgcagc tgttccgcga ccacggactg cccgctaccg
      481 atgtctatgc aatggcgcag gtagaaggcg cgggtaagcc gctgagtaat ttgcaaaatg
      541 ggcagatggt gaaaatccgc cagaacgcca gcggcgtggt gacgggctta accattgata
      601 cggggaataa tcaacaggtc ctgtttaccc gccagccgga tggcagtttt attcgtgcgc
      661 ggtagatgaa gggtcaacca aaagccagct aaggattgtc tctttgatga ccttacgtct
      721 ggcttccagt gcctggggtg aaaccatact gatgtgataa accaggccaa gcgtatgctg
      781 gttgatcaaa taatatcccc caagcgcagc aatattaata ttcacctgca atgggtcgat
      841 atctggctta aaaatattgt gtttcttccc ttcatccaat aaggattcca ttaactgcaa
      901 gtgcgcatgg ttaatttcta agagacgcag tgattttgca taatgcacac ctttactttg
      961 attttcacta tgcacaattt ttaaaaacca agggtt
//
LOCUS       DAJVRF010000326          987 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJVRF010000326 DAJVRF010000000
VERSION     DAJVRF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.331"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            1..909
                     /locus_tag="OQN13_004156"
                     /pseudo
     CDS             1..909
                     /locus_tag="OQN13_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          388 a          184 c          146 g          269 t
ORIGIN      
        1 attaatgcgt gccacacgaa cactacatat acaaatgcag actactcaaa agttgtggct
       61 caacttgtaa gtcttattac taacattgaa acgataagtt cgacacaact tacgcaacag
      121 acacaatcaa tacttaatca gattaataac attagatatg aaaaaaacaa aagtgctgaa
      181 tgtcgaatca ttgttgtcgc taatcctaaa cccgacaagg caatcataac taaaattagc
      241 gtagaagagg gaatacccat aacattcagt gtccaaacca tgttttcaga tactaatttt
      301 attgctgaac aacgagctga cttaccaact aatataaaag atattcaaag cctgtatcaa
      361 aaaatgacga agttatatat tgagcacagt gaaaataaaa acaggatgaa agtctttgcc
      421 ggagccaact ttattgattt caatatgaca ggacagaacc tgtccgggtt cgttctaacc
      481 ttgtcaaggt tttatttcga agatctactt aatataaatt tcactgacgc aaatttaggt
      541 gacactattt tttacataaa gaacacccta cccccaaatt atataaagat ggacaatatc
      601 ttgacaaaca aatcgaaggt ttattttcaa cattattaac cattaatgac aactttttac
      661 gagcaaaagc ggaaattgct tcgacaataa ttaaattttt agaggccagg ataaccaatc
      721 tttcatacaa tgacatatta aaatatcagc aagaattcca aaaacaatgt tataagcaag
      781 ttaaagcctt tacgacactc tctcgataca ataaaattca aacatgggca gaaatgtctg
      841 aatatcagtt cgaagtattt caatacgaga cgcttaaccc caaaaaaatg tcacacacgc
      901 cttatttaaa gaggccactt cctaatgaaa aagatataaa ttatggggtg gaaatagaaa
      961 taccatctgg caaaagaatt cgcttat
//
LOCUS       DAJVRF010000327          958 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJVRF010000327 DAJVRF010000000
VERSION     DAJVRF010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.332"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..680)
                     /locus_tag="OQN13_004157"
     CDS             complement(<1..680)
                     /locus_tag="OQN13_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1617337.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          291 a          159 c          199 g          309 t
ORIGIN      
        1 aacaaagttt gcattattct gggagatagt tgattttctc taagattgac aacgctatca
       61 gaactgaggc caagtatgct ttccggaatt gccgtgatcc ggttctgatt aagccagaat
      121 attcttataa tatgatcatc tctttctgga aaatctggta ttacttccaa agcatttctg
      181 gctgcatcaa gtaattccaa tgacattggt aacctcggaa gtacagacag gtgattgtca
      241 cttacattaa tatattctag tgacgcaggt aattcaggaa gtgcgaacaa atggttatca
      301 ctcacattta tatattccag cgacactggt aattcaggaa gtgcagataa ttgattactg
      361 cttgcattca gctctttcaa tgcccttggt agctcgggga gcattgatag ttggttattg
      421 cttacattaa tttcatcaag attgtcaggc aatcgtggta gagatctgag acctaaacaa
      481 gataagtcca acgatgtttc actgttctcc agacataatt ggagccgggt aaaagcagtt
      541 tccctgtttt ctccggaaat gctgttttta gtccattcaa cccattcgga gagataatta
      601 ttgtgaacat tgtcgattga tgtagttctg taaaaagaga cgtttccagt ggataggggg
      661 ggattattta caggaagcat aataacctcg cagaaggata ttctgataaa atgtgatact
      721 gggaaataat aagctgaaat aaaatgtttc aggattgaaa tttattgttt tcattttaag
      781 gaagagatgt tgatttaact gtaaaaaaac gatactattt tcttagatgg taaatgtctc
      841 gcctgtgcta tattttgttt ttcggttagt taaatatcgt acgttcatat ttgaacgttc
      901 tgccggaatg cattatcaat agaggtaaag tcgcaacccc aaatcgtaaa ggaaaccg
//
LOCUS       DAJVRF010000328          943 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJVRF010000328 DAJVRF010000000
VERSION     DAJVRF010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.333"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..267
                     /locus_tag="OQN13_004158"
     CDS             <1..267
                     /locus_tag="OQN13_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1617338.1"
                     /translation="YDYAAWLNPGFKYAAYKVLDDYSTGELQHRNSLSAQLNMKCHEF
                     DQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITIPGLPG"
     gene            264..521
                     /locus_tag="OQN13_004159"
     CDS             264..521
                     /locus_tag="OQN13_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617339.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
BASE COUNT          276 a          213 c          249 g          205 t
ORIGIN      
        1 tacgattatg cggcttggtt aaaccccggg ttcaagtacg cggcctataa agtcctcgat
       61 gactactcca ccggagaact tcagcatcgc aacagcttaa gtgcgcagct caacatgaaa
      121 tgtcatgaat ttgaccagaa gaaagatatg gcgagcttct gcggacaagg gctggcagca
      181 tggcgctata cgaagccagt gttggtcgct gagattaact ccctggctaa ccagctgcag
      241 attacgatcc ccgggcttcc gggatgagtg atcatgttat tgaatgcgcc tccagagcgg
      301 ggcgcgactt ctcagagttc atgaaaggcg agaagggcat gatggaagca ttggcctcgg
      361 tggatgagtt tggcgagcag ctgcgcctca acggctgtgt caatcatcac tttgttagct
      421 acatgatgcg gaactcgatc atgcaggcat tcatggacat ggcaaaagcc gagaggaaag
      481 aagagcgccg gcgtaagcga gcggaagcaa aagcgaagta gccattacaa agcccattta
      541 cgggtgggct tgataatgaa accggaattt attctgggcc accagttaac ggcagtacca
      601 cgaaacaacc caagccagta agtggggaaa taacaccggc agccactgaa agatgaacct
      661 cctgccttat ggcaaaaaag attctttgtg gtggcggact gatggaaaga catcccaatt
      721 tcagccaaac attgaaggag ttgttatgtc agcagaaggt ttcaataacc catcaaaatt
      781 ccgggatgag tgggatagca gcgtaaagag taagtgatgc catcacaaaa gccattccct
      841 acagagtggc tttgataatg gcttataccc tacacgggat aacttaactg atatcccttt
      901 taaaggataa aggtattcaa gcctgacaca tcatgcgctg tat
//
LOCUS       DAJVRF010000329          943 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJVRF010000329 DAJVRF010000000
VERSION     DAJVRF010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.334"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>943
                     /locus_tag="OQN13_004160"
     CDS             <1..>943
                     /locus_tag="OQN13_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HCS1617340.1"
                     /translation="TSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPL
                     LQSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAF
                     FAGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDR
                     NFWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMA
                     IIPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFK
                     YSVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGIL"
BASE COUNT          201 a          189 c          226 g          327 t
ORIGIN      
        1 ttaccagcat tctatttatg atgttctacg gcatcgttca ggataaactc ggtctgaaga
       61 aaccgctcat ctggtgtatg agtttcatcc tggtcttgac cggaccgttt atgatttacg
      121 tttatgaacc gttactgcaa agcaattttt ctgtaggtct aattctggga gcgctctttt
      181 ttggcctggg gtatctggcg ggatgcggtt tgcttgacag cttcaccgaa aaaatggcgc
      241 gaaattttca tttcgaatat ggaacagcgc gcgcctgggg atcttttggc tatgctattg
      301 gcgcgttctt tgccggcata ttttttagta tcagtcccca tatcaacttc tggttggtct
      361 cgctatttgg cgctgtattt atgatgatca acatgtgttt taaagataag gatcaccagt
      421 gcgtagcggc ggatgcggga ggggtaaaaa aagaggattt tatcgcagtt ttcaaggatc
      481 gaaacttctg ggttttcgtc atatttattg tggggacgtg gtctttctat aacatttttg
      541 atcaacaact ttttcctgtc ttttatgcag gtttattcga atcacacgat gtaggaacgc
      601 gcctgtatgg ttatctcaac tcattccagg tggtactcga agcgctatgc atggcgatta
      661 ttcctttctt tgtgaatcgg gtagggccaa aaaatgcatt acttatcggt gttgtgatta
      721 tggcgttgcg tatcctttcc tgcgcgctgt tcgttaaccc ctggattatt tcattagtga
      781 agctgttaca tgccattgag gttccacttt gtgtcatatc cgtcttcaaa tacagcgtgg
      841 caaattttga taagcgcctg tcgtcgacga tctttctgat tggttttcaa attgccagtt
      901 cgcttgggat tgtgctgctt tcaacgccga ctgggatact ctt
//
LOCUS       DAJVRF010000330          940 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJVRF010000330 DAJVRF010000000
VERSION     DAJVRF010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.335"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>940
                     /locus_tag="OQN13_004161"
     CDS             <1..>940
                     /locus_tag="OQN13_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HCS1617341.1"
                     /translation="TLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRH
                     WMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCH
                     LLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWG
                     VLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDL
                     ASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          272 a          222 c          277 g          169 t
ORIGIN      
        1 accctgctgg ccatcgatgg tgtgttctgg cgcacaccgg atacaccaga gaacgatgca
       61 gccttccccc gccagacaca tgccgggaac ccggcgctct acccgcaggt caaaatggtc
      121 tgccagatgg aactgaccag ccatctgctg acggctgcag ccttcggcac gatgaagaac
      181 agcgaaaatg agcttgctga gcaacttata gaacaaaccg gcgataacac tctgacgtta
      241 atggataaag gttattactc actgggactg ttaaatgcct ggagcctggc gggagaacac
      301 cgccactgga tgatacctct cagaaaggga gcgcaatatg aagagctcag aaaactgggt
      361 aaaggcgatc atctggtgaa gctgaaaacc agcccgcagg cacgaaaaaa gtggccggga
      421 ctgggaaatg aagtgacagc ccgcctgctg accgtgacgc gcaaaggaaa agtctgccat
      481 ctgctgacgt cgatgacgga cgccatgcgc ttccccggag gagaaatggc ggatctgtac
      541 agtcatcgct gggaaatcga actgggatac agggagataa aacagacgat gcaactgagc
      601 aggctgacgc tgagaagtaa aaagccggag cttgtggagc aagagctgtg gggtgtctta
      661 ctggcttata atctggtgag atatcagatg attaaaatgg cggaacatct gaaaggttac
      721 tggccgaatc aactgagttt ctcagaatca tgcggaatgg tgatgagaat gctgatgaca
      781 ttgcagggcg cttcaccggg acgtataccg gagctgatgc gcgatcttgc aagtatggga
      841 caacttgtga aattaccgac gagaagggaa agggccttcc cgagagtggt aaaggagagg
      901 ccctggaaat accccacagc cccgaaaaag agccagtcag
//
LOCUS       DAJVRF010000331          938 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJVRF010000331 DAJVRF010000000
VERSION     DAJVRF010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.336"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..629)
                     /gene="nac"
                     /locus_tag="OQN13_004162"
     CDS             complement(<1..629)
                     /gene="nac"
                     /locus_tag="OQN13_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCS1617342.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRR"
BASE COUNT          260 a          227 c          201 g          250 t
ORIGIN      
        1 cgccgtaagg aaaaagcctc atcaacacga agtctaatag cactgtaatc actggggaga
       61 aagaggttca ttttcgcaat agcattcaca tcaacgcttt gccccgggca atcttgagtt
      121 cctaccagaa aaagatcttc tttcagcaaa gcctgactgg atacaccagc cacaggggaa
      181 tgctcataaa tcaccgccat atcgagttgg tgatttatca atttttcgtt aagcactgca
      241 ccactatttt catgaagata gataacgatc tccggaaatt cagcgcgaac cgcctgtaat
      301 aagggcatgg tgatggatga cgcagcggtt cctggtgcaa agccaatcga gacttgcccc
      361 gataatgcct gaccaacgtt atgcaccgcc agttgggcct gttcacactg acgtaaaatg
      421 gcccgcgcat gggtatagag aatttttccg gcgtctgttg gcgtaacgcc ccgctttgta
      481 cggatcaaaa gttgttgatt taactcacct tccagtgtgg caacctgctg gctgagcgct
      541 ggttgtgcga tatgcaatac ttcagcagcc tgggtcaggc taccaatatc tacaattttt
      601 acgaagtatt tcaggcgtct gaagttcatg ttgcctccgg tttttaagaa tcggcccaag
      661 tgccgccatt acttacaacc agattgcaag atgcttgcca gttttatttt ggcgttgatg
      721 tacaagctaa ccaactgtca aataagagat tatgatagat tcgtcatttg ctcctttaat
      781 cagctgtcgc gttcccctgc cctataaaag gagggtatgc accacgatgg ttcattaccc
      841 aataagattg aaagctcacc actttgttga aattgacagc aaacaaacaa aaaaatgcat
      901 ttcacccttt gacatcacca tgcactgcca ttaatatg
//
LOCUS       DAJVRF010000332          908 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJVRF010000332 DAJVRF010000000
VERSION     DAJVRF010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.337"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..506)
                     /locus_tag="OQN13_004163"
     CDS             complement(<1..506)
                     /locus_tag="OQN13_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617343.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSPLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASY"
     gene            680..>908
                     /locus_tag="OQN13_004164"
     CDS             680..>908
                     /locus_tag="OQN13_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617344.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKK"
BASE COUNT          274 a          192 c          199 g          243 t
ORIGIN      
        1 aaataagatg ccatccattg gtacagcttc agctcgcgct gccttaacat ttttatccaa
       61 ccgttaccgc ccgtggtttt tgccaattgc gggccatcaa agcgaaaacc cggcacttca
      121 acctgcgggt agcggtcggt gatgagcaga aagccttgct ggcttttaca caacattttg
      181 cgaaacttgt acgcccgcca gcaggaaagc agatagataa ccagagcagt aatattgcca
      241 ggcggtgttg agggtttttc gtgcacatgt gccgctttac ttcgcagata acgcccaaaa
      301 ggtgcgccaa taacagggag ctgtgaaatc cattcgccaa ttagcccgga cgattgcccg
      361 agataaatgt gttctgttgg cattcttgct gccagttcat ttaccaggct tgccgtgagg
      421 ggcgatttac ctgaaccatc acatcctaca atggcaataa cacgcactgg cgtggaatta
      481 attattgaca tattacgttg attcacgaaa aacccggcag taaataatgt attgaatatt
      541 acgtggtccg tgctcagaat atccgttcaa ccttattgtg acaatggtta attgtgacat
      601 tgacctgaat tacgctttca taaaaacata ttaaccaaat aaatattttt aatggatgtt
      661 taaattaaag gatatattca tgcagtcaat aacacctcca ttaattgccg ttattggtag
      721 cgatggttca ggcaagtcaa cggtgtgtga acatcttatt accgttgtcg aaaaatatgg
      781 tgctgccgaa agagttcatt taggaaaaca ggccggaaat gtcggtcgtg cagtgacaaa
      841 attaccgttg atgggaaaat ccttacataa aacaattgaa cgaaatcagg tgaaaacagc
      901 aaaaaaat
//
LOCUS       DAJVRF010000333          904 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJVRF010000333 DAJVRF010000000
VERSION     DAJVRF010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.338"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..873
                     /gene="cbl"
                     /locus_tag="OQN13_004165"
     CDS             <1..873
                     /gene="cbl"
                     /locus_tag="OQN13_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCS1617345.1"
                     /translation="FTSQSGVSRHIRELEDELGIEIFVRRGKRLLGMTEPGKALLVIA
                     ERILNEASNVRRLADLFTNDTSGVLTIATTHTQARYSLPEVIKAFRELFPEVRLELIQ
                     GTPQEIATLLQNGEADIGIASERLSNDPQLVAFPWFRWHHSLLVPLDHPLTQITPLTL
                     ESIAKWPLITYRQGITGRSRIDDAFARKGLLADIVLSAQDSDVIKTYVALGLGIGLVA
                     EQSSGEQEEENLIRLDTRHLFDANTVWLGLKRGQLQRNYVWRFLELCNAGLSVEDIKR
                     QVMENSEEEIDYQI"
BASE COUNT          230 a          199 c          239 g          236 t
ORIGIN      
        1 tttacctcac agtccggcgt cagccgtcat attcgggaac tggaggatga acttggcatc
       61 gaaatatttg ttcgacgagg taagcgactg ctgggcatga ctgaaccggg caaagcatta
      121 ctggtcattg cagaacgtat tctgaatgaa gccagtaatg ttcgtcggct tgcagacctg
      181 tttaccaacg atacgtctgg cgttctcact attgcaacga cgcatactca ggcacgttat
      241 agcttgccag aggtcattaa agcttttcgc gaacttttcc cggaggttcg gctcgagcta
      301 atccagggga cgccacagga aattgcgacg ttgttgcaaa atggcgaagc tgatattggt
      361 atcgccagcg agcgtttgag taatgacccg cagctcgtcg ccttcccgtg gtttcgttgg
      421 caccatagtt tgcttgttcc actcgatcat cccttaacgc aaattacacc attgacgctg
      481 gaatcaatag cgaagtggcc gttaatcact taccgacagg ggattacggg gcgctcacgt
      541 attgatgacg catttgcccg caaaggtttg ctggcagata ttgtattaag tgcgcaggat
      601 tctgatgtca ttaaaaccta tgttgctctt gggcttggga tcggattagt tgccgaacaa
      661 tccagcggcg aacaagagga agagaattta atccgtctgg atacgcggca tcttttcgat
      721 gctaatactg tctggttggg actgaagcga ggacaacttc agcgtaacta tgtctggcgc
      781 tttctggaac tttgtaatgc aggactgtca gttgaggata tcaagcggca ggtgatggaa
      841 aacagtgaag aggaaattga ttatcagata tagcaaaaag cccgctataa aagcgggctt
      901 ttca
//
LOCUS       DAJVRF010000334          901 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJVRF010000334 DAJVRF010000000
VERSION     DAJVRF010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.339"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..>901)
                     /locus_tag="OQN13_004166"
     CDS             complement(<1..>901)
                     /locus_tag="OQN13_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HCS1617346.1"
                     /translation="IIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCHAS
                     RQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFANNT
                     VTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNAMF
                     RNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSKHP
                     PGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAG
                     YGDGPSTAAGGFMYLGLSEVTFDI"
BASE COUNT          251 a          215 c          188 g          247 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 c
//
LOCUS       DAJVRF010000335          890 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJVRF010000335 DAJVRF010000000
VERSION     DAJVRF010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.340"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..429)
                     /locus_tag="OQN13_004167"
     CDS             complement(<1..429)
                     /locus_tag="OQN13_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCS1617347.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHD
                     TLLPRFTDDISRQSWVSEKVFCNPPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNRLTIRSPFPSAVVVF"
     gene            complement(798..>890)
                     /locus_tag="OQN13_004168"
     CDS             complement(798..>890)
                     /locus_tag="OQN13_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1617348.1"
                     /translation="VQMLISQGKTRLQIQEELGISRATYYRLAK"
BASE COUNT          270 a          180 c          162 g          278 t
ORIGIN      
        1 aaaaacaacg actgcacttg gaaacggtga cctaatagta agcctgttat gccctgctgg
       61 tggaaggtat ttaactgcac gatgaagaat acggatttca tgacagtggt tattgctgta
      121 aatgtgccta agccagtagc tggtgttggc tctatgcggt atcaggaaag ctactaaatc
      181 cgcttcagac gcttttaaga ggaacgacgg aatatcgctg tacggcgggt tacagaagac
      241 cttttcacta acccatgatt gacggcttat atcatcggtg aatcgtggta acagcgtgtc
      301 atgctcagtt gcagcaccat ctagactgaa aataaattca cggtgtaagt tcttaaataa
      361 caaaaatgga gttctccagt aatcagaacc cccatgatgc ttcccgatta aaatatgctc
      421 aattgacata agaaataaaa atttttattg caatcactct tgacaaggtt tactctcttt
      481 taagagatgc ttaacaggtt ttctaaacgg atatcctttc tgggatatct atcttttatc
      541 ttaaaagttt ttacagatac tttaaagata tctgtatata ttctgtacag taacttttca
      601 gagtctgaaa atttttataa aaaatgacta gccctctttg aatctcatct tccccgaatc
      661 ttcgaaaata tctgtctagt tgattcggta gaatctacct catgatgtat acgtgtttag
      721 tacccgtcac accagctatg tgaccccctt ggcaaaaaag ccaaatatca gctacatcca
      781 tgtaactatt cagtaactta tttagcaagt ctgtaataag ttgctctaga gattcctagc
      841 tcttcctgaa tctgcaacct cgttttacct tgtgaaataa gcatctgtac
//
LOCUS       DAJVRF010000336          889 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJVRF010000336 DAJVRF010000000
VERSION     DAJVRF010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.341"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..363
                     /locus_tag="OQN13_004169"
     CDS             <1..363
                     /locus_tag="OQN13_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617349.1"
                     /translation="NKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKATLNRS
                     GKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSPDSEEW
                     SSQKASKDVEGIVFGGKK"
BASE COUNT          301 a          146 c          157 g          285 t
ORIGIN      
        1 aacaaagata aatatattaa caagttcaag aaagagcttt caaaatatat acaaccaact
       61 gctggagtgg acattcaatt atttaactct aaggatggtg gtggtgtaat taaagccact
      121 ttgaatagaa gtggaaaaag aaaatcatct attgctggta attttactaa attaggtgag
      181 gcaattacct cgagtgggca gagggttttt ggtggtgatc tatcaaatgt gaatttttac
      241 ggaacaaata cgatttttga tggagataca atatttttaa ttaaatcacc tgattcagag
      301 gagtggtcat cacaaaaagc ttccaaagac gtagagggca tcgtatttgg agggaaaaaa
      361 tgattaacat tactactaag gatgcactca agtcagtggt gaagtaagag atctagtaat
      421 tcttcttgaa actgatggaa aaacggtgtc tgattatttg cagccaatcc ctcccaaaaa
      481 agatacacac tggagcatga ttttcattcc ggtagcaatt tatatactac ttaatcttgt
      541 atacattatt gctcacccat tatttcctga aaaatatcta aaaacatcaa catctatatt
      601 tctattcatt tccttcatat taagtgcaat agtagcatat tttgttttcc ataagcataa
      661 atctgccact ctatccatat gcagcttagt gttcttagtg ttaattgtag gtgctaacgt
      721 agggtttatc agttacagcg acctaacaca aaaagcgacg caaaaattag atagtttgtc
      781 taaataatta tgttgccatg cgaagtatgc atggctgcat gtctgccttc catttaaaat
      841 gggctaagac ctataaccct aaatattatt ctttattatc ttctttacc
//
LOCUS       DAJVRF010000337          874 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJVRF010000337 DAJVRF010000000
VERSION     DAJVRF010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.342"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(58..>874)
                     /gene="treF"
                     /locus_tag="OQN13_004170"
     CDS             complement(58..>874)
                     /gene="treF"
                     /locus_tag="OQN13_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HCS1617350.1"
                     /translation="SLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAA
                     SGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQ
                     KASARRDAVNRYLWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANA
                     VRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKT
                     VNQFYLEQHKMIEKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          192 a          253 c          231 g          198 t
ORIGIN      
        1 ctcctgaaga ggataatttt acagccagca ggaagttagc ggctggctgt aaaaatatta
       61 tggttcgccg tacaaaccaa ttaaacggcg taccacaccg ttagtccagc caaacccatc
      121 ctgcaacgga tactcgccac cgccgccttc gcggggaaca ccatcggcaa tatggtattt
      181 ctcgatcatt ttgtgctgtt ccagatagaa ctgattcacc gttttcagcc agctgcgcgc
      241 gatttcatca cccagaaggt catcgccata cattttaaat ccctgaattg ccatccattg
      301 taacggtgcc cagccgttgg gtttatccca ctgttcaccg gtttcgtact cgcttgccag
      361 aatcccgcca ggtgtcagta accggctgcg tacggcgttt gccagacgat cggcctgttc
      421 atggttcgcc atgccgacat agagcggcac aatggcggca gcggaaaaca gcgccagttg
      481 ttcgcgtcgc cagtcgtaat cgcggtagat gccgttttca tcatcccaga ggtaacggtt
      541 taccgcatcg cgacgggcac tggccttctg gcggaacagc gcttctgtct ctttctcgcc
      601 tttcagcgcc gagatgttgg cgatggcgct ctccagttta aacaggaagg cattcagatc
      661 gatggggatg aactgggtgg tacgaatgct cgccagacga ccagtatcac gcagccaacg
      721 ggaagagtaa tcccaaccgg aggccgcccc cgcgcgtaaa tcgcggtaca cctcgttggg
      781 cgggcgacca gaatgtttcg cggtttcaac gtcctcaagc caggattcgt cacgcggcgt
      841 gtcgcgatcg tcccagtaac ggttgagcag cgat
//
LOCUS       DAJVRF010000338          868 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJVRF010000338 DAJVRF010000000
VERSION     DAJVRF010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.343"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            62..295
                     /locus_tag="OQN13_004171"
     CDS             62..295
                     /locus_tag="OQN13_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCS1617351.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(296..745)
                     /locus_tag="OQN13_004172"
     CDS             complement(296..745)
                     /locus_tag="OQN13_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1617352.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
BASE COUNT          250 a          231 c          218 g          169 t
ORIGIN      
        1 ccacgcagac ttatatcatt tggatgaatc gataaatttc acaagtggtt aaggagaaag
       61 tatgtcgcat ctggatgaag tcatcgcgcg cgtggatgcc gcgattgaag agagcgtcat
      121 tgcccatatg aacgaattat taatcgccct gagcgatgac gcggagttaa gtcgggaaga
      181 tcgctacacc cagcagcagc gtttgcgcac cgctattgct catcacggtc gcaagcataa
      241 agaagatatg gaagcgcgcc acgaacagtt aaccaaaggc ggcaccatcc tctgattaaa
      301 atgagcgtct ggctaccaac caggcgccaa ccacaagtaa aattgcgcca caaattgggc
      361 cgaacaacgc ccgtagtggt acgccatgac tgcggaagat atccagcatc agaccgccaa
      421 ttagctggct ggcgaccagt actgcaatag ttgtcgccgc tcccacattc tgatatccgc
      481 tgatactggc gaagacaaaa aacgatccca gcaagccggg tataagtgtc caccagcgca
      541 cgctggacac cagttcgcca aaccccgcca tgccttgttt aaaccataaa atagaaacaa
      601 agagcacaat ccccaccagt gagttcagca acatggcgat gagaatggta gaggaggtct
      661 gggtgatgcg caccatcagc gtgttctgga ctaccagacc aattccggcg gcaatcaaaa
      721 aagcaagggt gagcgactga ttcatccaat cgcgtccggt tcagtgcgtt cgtcgagttg
      781 caactgcata aatgtcagat ccagccagcg cccaaattta gtgcccacct gcggcatttg
      841 tgcggtgacg acaaatccca gcgaatgg
//
LOCUS       DAJVRF010000339          865 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJVRF010000339 DAJVRF010000000
VERSION     DAJVRF010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.344"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..423)
                     /locus_tag="OQN13_004173"
     CDS             complement(<1..423)
                     /locus_tag="OQN13_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1617353.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQ"
     gene            complement(546..>865)
                     /gene="tabA"
                     /locus_tag="OQN13_004174"
     CDS             complement(546..>865)
                     /gene="tabA"
                     /locus_tag="OQN13_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCS1617354.1"
                     /translation="YLISEDMTEPHEARRTEYHARYLDIQIVLKGQEGMTFSTQPAGT
                     PDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKM
                     LMA"
BASE COUNT          251 a          201 c          153 g          260 t
ORIGIN      
        1 ttgaaatata atagccgtat gtccttgacg agtccccata gtagtgaaag cggaaagtgt
       61 gatttctatt tccaccttag ataaatccgc tcgttcccct tcaggattat tcataacaaa
      121 cttcattatc tcactgccat cattattttt gatataagat gaggactcaa tatgagccat
      181 catatttctt cccgcttcgt tggagcgttt gctaaaatcc acataaccta gagtaaatat
      241 ttttaccaaa aattccaaaa atgtcttagg atcaaggata tttcttgtgt tatcctgccc
      301 ctcggcatta cgggcaaaat tattcattcc aacacgccag gtatttaaaa cttcactttt
      361 ttgtttaaaa ttgtcggcta atgtaatgtg ttgagaataa ttcaaatctg atattttgct
      421 catgacgaac tccttgagtt aataagaacg tctaagcatt acaaatttta ctcacaaaaa
      481 aatatcaaaa aacgctcttt tcacaaactc gccgtaaacc tggagttatt tacggcgaaa
      541 agtcatcacg ccatcagcat cttcactact gctttgcgaa cctgggctgg tgcacccact
      601 gcgcacagcg gtttatgcac ttcccccgga taaaacacaa caaaatcacc ttcattaagg
      661 ataactgttt tctcatcaac gccttccggc aaaaatgcga tgtctttatc agctaaccag
      721 tcggtatccg gcgtgcctgc aggttgcgtg ctgaaggtca tgccttcctg accttttaac
      781 acaatctgaa tgtcgagata gcgggcatgg tactccgtac ggcgagcttc gtgcggctcg
      841 gtcatatctt ccgagataag ataaa
//
LOCUS       DAJVRF010000340          864 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJVRF010000340 DAJVRF010000000
VERSION     DAJVRF010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.345"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            1..262
                     /locus_tag="OQN13_004175"
                     /pseudo
     CDS             1..262
                     /locus_tag="OQN13_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            377..>864
                     /locus_tag="OQN13_004176"
     CDS             377..>864
                     /locus_tag="OQN13_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCS1617355.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNA"
BASE COUNT          329 a          173 c          116 g          246 t
ORIGIN      
        1 tgatttgagt gaattaccgc ctatcccctc gacagttaat acgttaaatc tggaaaacaa
       61 ttgtctaact tgtcttgact tagctgacaa tgccagtctc gttaatatca acctcagcgt
      121 taataaaatt aacacgataa cctttccaaa agaatcaaaa ctggaaaaca tttatattga
      181 tcataacaat ctggaaagtt tagatttaaa aaatcagcat tcattggtta atctggaagc
      241 gcaaaataac aaactgacaa aaattaatat ttctgatagt tataaactga aatttcttaa
      301 tcttgattat aacaagctag catcgctgga tgtctcccgg caagaatctc tgattgagtt
      361 aagtgcccat cacaatatga tcactgacct tatattacac aatcatccca ggatgaaaac
      421 aatcacttta aacgacaacc atattgcaca tttaaacgcc aaaaacacta caaaactgga
      481 atatttaaac ttaagcaata acaatttact gccaaccaat gacattgatc aactaatatc
      541 atcaaaacat ctttggcatg tattagttaa cggcatcaac aatgatccac ttgcacaaat
      601 gcagtactgg actgcagtaa gaaatataat tgatgacact aatgaagtga ccattgattt
      661 atcaggactt aatttaacca ctcaaccacc agggctgcaa aacttcacat ctatcaatct
      721 tgataataac caactcacac attttgatgc aaccaactac gatagactcg taaagctaag
      781 tctgaatagt aatgctcttg agtcaataaa ttttcctcaa ggcagaaatg taagtattac
      841 acatatatct atgaataata atgc
//
LOCUS       DAJVRF010000341          849 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJVRF010000341 DAJVRF010000000
VERSION     DAJVRF010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.346"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..659
                     /locus_tag="OQN13_004177"
     CDS             <1..659
                     /locus_tag="OQN13_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCS1617356.1"
                     /translation="ADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDYTIF
                     FYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGIRCT
                     MCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWDYNW
                     RKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKEEKE
                     "
BASE COUNT          227 a          190 c          220 g          212 t
ORIGIN      
        1 ttgctgattt caaacggccc aaattggagc tcccaaacgg ggcaaacaaa ctactactgc
       61 actcttgctg tgctccatgt tccggtgagg tgatggaggc gcttcaggcc tcgggaatcg
      121 actacaccat ctttttctac aacccgaaca ttcatcctca gaaagagtat ttaattcgta
      181 aggatgagaa tattcgcttt gctgaacaac acggcgtgcc gtttattgat gctgattacg
      241 acacagacaa ctggtttgaa cgagccaaag gaatggaatg ggagcccgaa cgagggatcc
      301 gttgcaccat gtgttttgac atgcgttttg agcggacagc gctgtacgcc gctgaaaatg
      361 gtttcagtgt gatcagcagt tcactgggca tttcacgctg gaaaaatatg cagcaggtta
      421 acgactgtgg gcggcgagcc gtcgcgcatt atccgggcat ggtgtactgg gattataact
      481 ggcgcaagca gggcggctcg tcccgtatga ttgaaatcag caagcgcgaa aaattctatc
      541 agcaggaata ttgtggctgt gtgtattctc tgcgcgatac caatctacac cgcaaatctc
      601 agggacgccc tcttatcaaa attggtcaac tccactacgg taaagaagag aaggagtgat
      661 tttatggggc acctttctga ttgatttcat attggcgagg taagtagaat gactgcgggt
      721 gcttgaggct atctgcttca ggcatgaaca ccaaaaggca gatagagaaa agccccagtt
      781 aacattacgc gtccggcaag acgcttaaca ttaatctgag gccaatttca tgctttgcac
      841 atgtaggtt
//
LOCUS       DAJVRF010000342          834 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJVRF010000342 DAJVRF010000000
VERSION     DAJVRF010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.347"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(156..>834)
                     /locus_tag="OQN13_004178"
     CDS             complement(156..>834)
                     /locus_tag="OQN13_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCS1617357.1"
                     /translation="DVFMTYRTTGLATYRNYDFFGLIEGLNFAAQYQGKNERTDNSHL
                     YGADYTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQERAAANPLNASGKNAELWA
                     TGIKYDANNIYFAANYAETLNMTTYGDGYISNKAQSFEVVAQYQFDFGLRPSLAYLKS
                     KGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYVDYKINLIDESDFTRAVDIRTDNIVA
                     TGITYQF"
BASE COUNT          238 a          172 c          193 g          231 t
ORIGIN      
        1 aaatattata gtaagatgtc cccggaggat atggaataat attaattgag gtgaatgacc
       61 agaaatatca aaaattgctc atttttactt aaggagtcat acagcagcac ttaaatcaaa
      121 aaccagccca gagaagggct ggtcaggaga ggacgttaga actgataggt aatgcccgta
      181 gcgacgatgt tatcggtccg aatatctacg gcacgggtaa agtcgctttc atcaatcagg
      241 ttgattttat aatcaacata ggtcgacata tttttgttga agaaatacgt cgcaccaacg
      301 tcgatatact caatcatgtc ctggtcgccg tagcggccca gatctctgcc tttcgatttc
      361 aggtaagcga gtgatgggcg caagccgaag tcgaattgat attgcgccac cacttcaaaa
      421 ctttgtgctt tgttagagat ataaccatcc ccataggtgg tcatgtttaa tgtttcagcg
      481 taattagctg caaagtagat gttgttggca tcatacttta tacctgtggc ccatagttct
      541 gcattcttac cggaggcatt gagaggatta gcagcggctc tttcctgcgc atttgtccga
      601 tcggatttgg tatataccgc accgatacca aagccatcat aaacataagt tgaggagata
      661 ccgaaaccgt caccgttggc acgcgtgtag tcagcaccat aaagatgact gttgtcagta
      721 cgttcatttt tgccttgata ttgcgcggca aagttcagcc cttcaattaa gccaaagaaa
      781 tcgtagttgc gataggttgc tagaccggtg gtacggtatg tcatgaagac atct
//
LOCUS       DAJVRF010000343          830 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJVRF010000343 DAJVRF010000000
VERSION     DAJVRF010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.348"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..209
                     /locus_tag="OQN13_004179"
     CDS             <1..209
                     /locus_tag="OQN13_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1364 family protein"
                     /protein_id="HCS1617358.1"
                     /translation="VLAHYRLAGTCGTAIKPHDMQAAIACSSCHDLIDGRVKTSDYTK
                     EELRLMHAEGVFRTQEIWRKEGYL"
     gene            206..760
                     /locus_tag="OQN13_004180"
     CDS             206..760
                     /locus_tag="OQN13_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCS1617359.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          248 a          155 c          236 g          191 t
ORIGIN      
        1 ctgtgctggc gcattaccga ctggcgggaa cgtgcggaac agcgataaag ccacacgata
       61 tgcaggcagc gattgcctgt agctcgtgcc acgatttaat cgacgggcgg gtaaaaacca
      121 gcgattacac caaagaagaa ttacgcctga tgcatgcaga aggtgttttt cgcacacaag
      181 aaatctggag aaaggaggga tatttatgat ttacccaacg aatacaggaa aaagcggaga
      241 acaccttcgt ctcaccacgc tggaaagtgt ctggattcag ggaaaactac gtatgtgggg
      301 gcgctggtcg tatattggtg gcggtaagac ggggaatatg tttaacctga tgttgacctc
      361 taaaaagctg acaaaaacgg caattaacga ggcgctccgg aggatgaaaa aagcaggtct
      421 gaacaagtct gaacttgagg cttttttgcg ggatatgatt aacggtaagc aaaagagctg
      481 gctggcgcat tgtactgatg cagaggcgtt atgtattgat cgggtcataa gtgaggtgct
      541 ggcagagcat ccaggattga ttagcgtcct tcggcaacgg tatgaggggc gggggatgac
      601 caaacgcaaa atggctgaac tgctgaatga tgcacacccg aaatggagtt taagaacctg
      661 tgaaagacgc attgagcatt ggctaaaggt ggcagaattt attttgtaca aaccaatggt
      721 tatggctttt ggtatagaga aaaaagttat tgctttttga cgtaaaaact gcttcaattc
      781 ttgtacgctt cgcaaagctg taccgcgagg cgaatagcag acatggacat
//
LOCUS       DAJVRF010000344          830 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJVRF010000344 DAJVRF010000000
VERSION     DAJVRF010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.349"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>830
                     /locus_tag="OQN13_004181"
     CDS             <1..>830
                     /locus_tag="OQN13_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HCS1617360.1"
                     /translation="KKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGR
                     RSRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNA
                     LLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREAL
                     HWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLG
                     LEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHS
                     "
BASE COUNT          199 a          202 c          257 g          172 t
ORIGIN      
        1 gcaaaaaagt ggatagcgca cgcgccctta ttgcccgggg atggggagta agcttagtca
       61 gccgttgtct ccgggtgtcg cgtgcgcagt tgcacgtcat tctcagacga accgatgact
      121 ggatggatgg ccgccgcagt cgtcacactg atgatacgga tgtgcttctc cgtatacacc
      181 atgttatcgg agagctgccc acgtatggtt atcgtcgggt atgggcgctg cttcgcagac
      241 aggcagaact tgatggtatg cctgcgatca atgccaaacg tgtttaccgg atcatgcgcc
      301 agaatgcgct gttgcttgag cgaaaacctg ctgtaccgcc atcgaaacgg gcacatacag
      361 gcagagtggc cgtgaaagaa agcaatcagc gatggtgctc tgacgggttc gagttctgct
      421 gtgataacgg agagagactg cgtgtcacgt tcgcgctgga ctgctgtgat cgtgaggcac
      481 tgcactgggc ggtgactacc ggcggcttca acagtgaaac agtacaggac gtcatgctgg
      541 gagcggtgga acgccgcttc ggcaacgatc ttccgtcgtc tccagtggag tggctgacgg
      601 ataatggttc atgctaccgg gctaatgaaa cacgccagtt cgcccggatg ttgggacttg
      661 aaccgaagaa cacggcggtg cggagtccgg agagtaacgg aatagcagag agcttcgtga
      721 aaacgataaa gcgtgactac atcagtatca tgcccaaacc agacgggtta acggcagcaa
      781 agaaccttgc agaggcgttc gagcattata acgaatggca tccgcatagt
//
LOCUS       DAJVRF010000345          827 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJVRF010000345 DAJVRF010000000
VERSION     DAJVRF010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.350"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>827
                     /locus_tag="OQN13_004182"
     CDS             <1..>827
                     /locus_tag="OQN13_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCS1617361.1"
                     /translation="DVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRKAWDV
                     LSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSALKDRH
                     NAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLWLIKT
                     ELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLDREIT
                     LPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTLHQR"
BASE COUNT          167 a          214 c          285 g          161 t
ORIGIN      
        1 cagatgtgga taaatgggcg ctgtatgtca tcggccagta ctgtgaccag tcggtgccgg
       61 acggctttgg cagcacggag ccgcgcatca cctgtaatgc ctggctgacc acacagcgca
      121 aggcgtggga tgtgctcagt gatttctgct cggcgatgcg ctgtatgccg gtatggaacg
      181 ggcagacgct gacgttcgtg caggaccgac agtcggataa ggtgtgggcc tataaccgca
      241 gtaatgtggt gatgccggat gatggcgcgc cgttccgcta cagtttcagc gccctgaaag
      301 accgccataa tgccgttgag gtgaactgga ttgacccgga taacggctgg gagacggcga
      361 cagagcttgt ggaggatacg caggccattg tccgttacgg tcgtaacgtc acgaagatgg
      421 atgcctttgg ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg ctgattaaaa
      481 cggaactgct ggagacgcag accgtggatt tcagcgtggg tgctgaaggg cttcgtcatg
      541 taccgggcga tgtcattgaa atctgcgatg atgactatgc cggtatccgc accggcgggc
      601 gcgtgctggc ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa atcacgctgc
      661 catcctccgg caccacgctg ataagcctgg ttgacgggca ggggaatccg gtcagcgtgg
      721 aggtccagtc cgtcaccgac ggcgtgaagg taaaagtgag ccgtgttcct gacggtgttg
      781 ctgaatacag cgtgtggggg ctgaagctgc cgacgctgca ccagcga
//
LOCUS       DAJVRF010000346          817 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJVRF010000346 DAJVRF010000000
VERSION     DAJVRF010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.351"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..596)
                     /locus_tag="OQN13_004183"
     CDS             complement(<1..596)
                     /locus_tag="OQN13_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617362.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYAYFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
BASE COUNT          237 a          173 c          143 g          264 t
ORIGIN      
        1 atcagcttag ccagtcttga tttcagcaaa ttaatctcat tttctcttgc tttcatcagt
       61 ttctctttgg caatttcttc aagctcagct tgtgatttat cagcaacaat tagagacagt
      121 cgatgtggtg ctctgacagc tctttctgta cgaacaactc tttgaccttc tggtaaatct
      181 gcaatcactt taccaatctc tacagggtca tacccccaac cgtcacggtc tagtcgagag
      241 aaataagcat aagaattggt ttgaagctcc atcgaaataa ccatttcgtc aatttcgtct
      301 tcaagcttgg actgaatttt ctcgtaaaca cttctcattg cttcttgaga gtctttaata
      361 gcttggaaat atcggcgttg tagcttcaac ttcaaatcaa gttcacaccg ttcgttcata
      421 gaaaaatcaa tttcaactct tggaatctcg agtggttctg gtggaatata tggaccttta
      481 tttacttcgt gtgattcccg ccaagctcta tcaaaagatt cacgcaaagt taattcgtga
      541 atatcaagtg cacttggtct gtgttcgata agcatttcat gttcatttct aatcattcgt
      601 tcacccctta gtttatttgt tatttcaatt tatagaagca cactcagcaa tcagaggtga
      661 agagtctgac tgaacaagaa cctgccaagt gtgcttttaa aaactgaaaa agccctaaac
      721 atttctgtga agggcaaagg attcaataat gaatgaacaa gagtactttt tgagatagcc
      781 tctcagacta acctgaaagg ccatgacgaa aagcatt
//
LOCUS       DAJVRF010000347          795 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJVRF010000347 DAJVRF010000000
VERSION     DAJVRF010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.352"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>795
                     /locus_tag="OQN13_004184"
     CDS             <1..>795
                     /locus_tag="OQN13_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HCS1617363.1"
                     /translation="ECQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNLTAVMFDH
                     VRMSTGNFKDCITEQLELTIDYSDIFGNEDLDGYINNIIKMIDTLPDNAMILKSVLAV
                     KLVMQLKILNIVNKNFIENMKKTFSHCPYIKDPIIRSYIHSGEDDKFDNFMRQHRFSK
                     VNFDTQQMIHFINRFNMNKGLIDKNNNFFIQLIDQALRSTDDMIKANAWYLYKEWIRS
                     DDVSPIFIETEEKLRTFNTNKLTRNDNIFILFSSVDDGPVMVVSSQR"
BASE COUNT          282 a          117 c          136 g          260 t
ORIGIN      
        1 cgaatgtcag attagaaatt caaacttcga taattgttat ctttacgctt cgcacttcac
       61 cagagcagag tttctgtctg ccaaagaaat atcatttatt aaatcgaatt tgacagctgt
      121 tatgtttgat catgtgcgaa tgtcgacagg gaattttaaa gattgcatta cagaacaatt
      181 ggaattaact attgattatt cagatatatt tgggaatgaa gatctcgatg gttatatcaa
      241 taacattata aaaatgattg atacattgcc agataatgca atgatattga aatccgttct
      301 ggcagtaaaa ctggtgatgc aattaaaaat tctcaatatt gttaataaaa actttattga
      361 gaatatgaaa aaaacattta gccattgtcc ttatataaaa gatccaatta tacgtagtta
      421 tatacattct ggggaagatg acaagttcga taattttatg cgtcaacatc gattcagtaa
      481 ggtgaatttc gatacccaac agatgatcca ttttattaac agatttaata tgaataaagg
      541 gctgattgat aaaaataaca atttttttat ccaacttatc gatcaggcct tacgatcaac
      601 ggatgatatg atcaaagcaa atgcctggta tctttataaa gagtggattc gtagtgatga
      661 tgtttcacct atatttatag aaactgaaga aaagttaaga acctttaaca ctaataaatt
      721 aacacgaaac gataatatat ttatcctgtt ctcctctgtc gatgatgggc ctgttatggt
      781 ggtaagctcc cagcg
//
LOCUS       DAJVRF010000348          788 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJVRF010000348 DAJVRF010000000
VERSION     DAJVRF010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.353"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            211..>788
                     /locus_tag="OQN13_004185"
     CDS             211..>788
                     /locus_tag="OQN13_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HCS1617364.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSP"
BASE COUNT          172 a          237 c          205 g          174 t
ORIGIN      
        1 ccttataatg gggtgttagc cagccagacc cggcatgatt actgccccca gtcgtccatg
       61 atccgggggg tgatgtcacc gggtctggtg gggcgctggt aaccgctaat aggggtcagg
      121 tcaggcactt ttgccgggac cgtctgtaac gtggatgccg gtacctgctc cccgtggtta
      181 tctggttaac ccatatacaa gggagacaga atgaccgaat ccagcgatta cgaatccgtc
      241 caggtcttta tcggcgttga tgtcggtaaa gatacgcatc acgctgttgc cattaatcgt
      301 tcaggtaaac gcctgttcga taaagcatta cccaacgacg aaaacaaact caggtcgcta
      361 atatctgacc tgaaacaaca tggtcagata ctgctggttg ttgatcagcc agctaccatc
      421 ggtgcgttac ctgtcgccgt tgcccgctca gaaggagtcc ttgtcggata cctccctgga
      481 ctggccatgc gccgcatagc cgacttacac gccggtgaag ctaaaactga tgctcgtgac
      541 gctgccatca ttgccgaagc tgcccgtacc ctgcctcacg cgctacgcac gctgaaactg
      601 gctgacgagc aaatcgccga actctccatg ctctgcggct tcgatgatga tcttgccgca
      661 cagacaacgc aggccagcaa ccgtatccgc ggccttctga cccagataca tccggcactg
      721 gagcgcgttc tcggtccgag acttgagcac ccggcggtac tcgatcttct ccagcgatat
      781 ccctcacc
//
LOCUS       DAJVRF010000349          775 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJVRF010000349 DAJVRF010000000
VERSION     DAJVRF010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.354"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            1..574
                     /gene="hipA"
                     /locus_tag="OQN13_004186"
                     /pseudo
     CDS             1..574
                     /gene="hipA"
                     /locus_tag="OQN13_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
BASE COUNT          202 a          149 c          206 g          218 t
ORIGIN      
        1 tgccacagga ggatatgtgt cagacattcg gtttaccttc atcggtgaaa tatgaatcag
       61 atggaggccc aggcatcgcg cggatcatgg cgtttttgat ggggtccagc gaggcgctga
      121 aagatcgcta tgattttatg aagtttcaag tgttccagtg gttgattggc gcaacggatg
      181 gccatgcaaa aaacttctcc gtatttattc aggctggcgg cagttatcga ctcacgccat
      241 tttacgacat catttcagca tttccggtcc ttggcggtac gggaataaca tcagcgatct
      301 caaactggca atggggctta acgcatccaa aggcaaaaaa acggcaatcg ataaaattta
      361 tccgcgacat tttttggcga cagcaaaggt gctgagattc ccggaagtgc agatgcatga
      421 aatcctaagt gactttgcca gaatgattcc ggcagcactg gataacgtga agacttcatt
      481 accgacagat tttccggaga acgtggtgac ggcagttgaa accaatgtgt tgaggttgca
      541 tggacggtta agccgagaat acggtagtaa gtgagatatg gggtttggtc gttgttaatg
      601 agcaatcatg accggccaac gactggattc tcccttggtt tatgtttgtg cgcataatgt
      661 atattatgtt aaatcatgtc tgagtgatgc gaagtgcttt tctggtagtc gttattcgtt
      721 cagattaggt gtccttgtga tttaaagatg ttatcgtggc acgaatatac tgcca
//
LOCUS       DAJVRF010000350          760 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJVRF010000350 DAJVRF010000000
VERSION     DAJVRF010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.355"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..538)
                     /locus_tag="OQN13_004187"
     CDS             complement(<1..538)
                     /locus_tag="OQN13_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617365.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGK"
BASE COUNT          244 a          142 c          139 g          235 t
ORIGIN      
        1 ctttcccttt agggaaaagt ctcagcaaag gcatatggtg ttcaagaaga atacatttgc
       61 aatctgttcg cactgaattg ataaactgca ctaatttctt aagcccggta aagcgtccag
      121 cctgcacatt tgctttaata tcaagaaacg catcatcctg gtcatatacc caaactgtcc
      181 ctttttgatt ttcaagtatg cgaaaaacaa acttaaccgg gtgatcaggg taatccatcc
      241 agtcataaaa tcctgggaaa tcattaaact gcacatttat ggtgagtgcg ttatcgtctg
      301 accactcaca gtctcgtacg gcagccatga gatgaatgag tttgtcgtca tcgactaaat
      361 catcacgaca atcataagga taatcataat attgtttaat caaaaccttg aatatatcga
      421 cacaggaaac tagggagctt ttgatatcct ctggtaactg attgttttta accgtattaa
      481 gtatgctttg ttgcgtatat ggcatcaaat tattgtgcgg agcttttaat gatatcatcg
      541 cgatctctct taaataaaaa taaacgtcgc agataataaa gcgctgatta aaccaggcac
      601 tatcagaaat cgctcataac taacgtgaga agtattgtgt actggaggga gttaatttaa
      661 caatttgaat ttacgttata ggttttactg ctactttgat attatttcgc tctccttaac
      721 taaaaagtta agtggagagc gaagttaata tgttaaacca
//
LOCUS       DAJVRF010000351          757 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJVRF010000351 DAJVRF010000000
VERSION     DAJVRF010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.356"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..392)
                     /locus_tag="OQN13_004188"
     CDS             complement(<1..392)
                     /locus_tag="OQN13_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCS1617366.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKP"
     gene            complement(389..>757)
                     /locus_tag="OQN13_004189"
     CDS             complement(389..>757)
                     /locus_tag="OQN13_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeA"
                     /protein_id="HCS1617367.1"
                     /translation="TVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLT
                     FADSAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSP
                     FGGVSITVELPLERDLQREV"
BASE COUNT          174 a          208 c          196 g          179 t
ORIGIN      
        1 tgcggtttgc aacggcgcaa aatggttttg acgcgcgcta ccacttcccg tgggctgtac
       61 ggcttgcaga tgtagtcatc tgcgccaatc tccagtccga gcaggcggtc gatctcttcg
      121 atttttgccg tcaccatcac gataggaatg tcagaaaaac gacgaatttc ccggcacagc
      181 gtcaagccat cggtgccagg gagcatcaga tccaacagga tcagatccgg tggtgtctgg
      241 cgcacatacg gcagtacctg atcgccgtgg ctgataagcg tcggcgcata gctcgcagca
      301 cgcagataat caatgagcaa ctgccccagc ttcggttcat cttccacgat caaaatacgc
      361 ggtgtgtttt cgtcgattgg taactcggtc atacttctct ctgtaaatcc cgttccagcg
      421 gtaactctac tgtaatgctt accccgccaa aaggcgaatg ggcagcaata atgcggccat
      481 tatgtgcttc aacaatgttc aggcaaatcg ccagccccag cccggaacca ccgctggcgc
      541 ggttgcggga accttcggtg cgataaaaac gttcaaataa tttttgtagc tgctcgtcac
      601 tgacacctgg cgcactgtcg gcaaaggtca ggcgcaccgt tttgtcgtgc tgctcggcag
      661 agattttcag gctgccgccg ctgtcagtgt agcgcaggct gttttcaagt aaattattga
      721 ataactgcat taaacggtcg cgatcgccaa atacggt
//
LOCUS       DAJVRF010000352          746 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJVRF010000352 DAJVRF010000000
VERSION     DAJVRF010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.357"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..142
                     /locus_tag="OQN13_004190"
     CDS             <1..142
                     /locus_tag="OQN13_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCS1617368.1"
                     /translation="SCGGVPISVIRQYIEQQQTPGQVENRALYPRPEGWGFTAHRIKS
                     AI"
     gene            complement(160..746)
                     /locus_tag="OQN13_004191"
                     /pseudo
     CDS             complement(160..746)
                     /locus_tag="OQN13_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          190 a          179 c          163 g          214 t
ORIGIN      
        1 tagctgcgga ggtgtgccaa tatccgtcat ccgccaatac attgaacaac agcaaacacc
       61 tggtcaggtg gaaaaccgcg ccttatatcc ccgccctgaa ggatggggtt ttacggcgca
      121 ccggataaaa agcgccatct aacgatggcg cgtctggaac tactttttgt taacgtcaaa
      181 catggtggca tcggtcgcca tgtcatcaat aacttgttta atattttcga aagccattgg
      241 cgtactttcg ttattcaggt ctttaccttc gccctgacga acgactttga taaccggttt
      301 attagtcgct gcatcaatca gctcaccttc aaaatagagt cgagtatcca tggtacggtg
      361 gcctgtagcc atttgcgtcc ccgcaaccac taatgcaaca ggaaccactt catagaattg
      421 cagcccttct ttgctggtat ctacaccggt aatggcccca cggaaaatca gactacgcgg
      481 cccagcggtg gtaaccagtg gtttacgctg cgctatcgct tctttcattt cggtgttggt
      541 atagttcaaa attttatcca gaactttctg ccctacctag gtagaaggtt ttggcaccgg
      601 ataataagtg attgggttcc agacgatgct gtcatatttg ctttgatcaa aactcgggtc
      661 tacccaacgt aaaacaggtt tacctgttgc tgaggttgtt tcttttaaat cagagtaatt
      721 gtttaaaaaa ccagaatatt tatctg
//
LOCUS       DAJVRF010000353          741 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJVRF010000353 DAJVRF010000000
VERSION     DAJVRF010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.358"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..454
                     /gene="csgB"
                     /locus_tag="OQN13_004192"
     CDS             <1..454
                     /gene="csgB"
                     /locus_tag="OQN13_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCS1617369.1"
                     /translation="KNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFNQ
                     AAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDAS
                     ISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            495..>741
                     /locus_tag="OQN13_004193"
     CDS             495..>741
                     /locus_tag="OQN13_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCS1617370.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALVLQTDARNSDLTITQHGGGNGADVGQ"
BASE COUNT          213 a          142 c          188 g          198 t
ORIGIN      
        1 gaaaaacaaa ttgttattta tgatgttaac aatactgggt gcgcctggga ttgcagccgc
       61 agcaggttat gatttagcta attcagaata taacttcgcg gtaaatgaat tgagtaagtc
      121 ttcatttaat caggcagcca taattggtca agctgggact aataatagta ctcagttacg
      181 gcagggaggc tcaaaacttt tggcggttgt tgcgcaagaa ggtagtagca accgggcaaa
      241 gattgaccag acaggagatt ataaccttgc atatattgat caggcgggca gtgccaacga
      301 tgccagtatt tcgcaaggtg cttatggtaa tactgcgatg attatccaga aaggttctgg
      361 taataaagca aatattacac agtatggtac tcaaaaaacg gcaattgtag tgcagagaca
      421 gtcgcaaatg gctattcgcg tgacacaacg ttaatttcca ttcgactttt aaatcaatcc
      481 gatgggggtt ttacatgaaa cttttaaaag tagcagcaat tgcagcaatc gtattctccg
      541 gtagcgctct ggcaggtgtt gttcctcagt acggcggcgg taaccacggt ggtggcggta
      601 ataatagcgg cccaaattct gagctgaaca tttaccagta cggtggcggt aactctgcac
      661 ttgttctgca aactgatgcc cgtaactctg acttgactat tacccagcat ggcggcggta
      721 atggtgcaga tgttggtcag g
//
LOCUS       DAJVRF010000354          737 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJVRF010000354 DAJVRF010000000
VERSION     DAJVRF010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.359"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..461)
                     /locus_tag="OQN13_004194"
     CDS             complement(<1..461)
                     /locus_tag="OQN13_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1617371.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLV"
     gene            complement(561..>737)
                     /locus_tag="OQN13_004195"
     CDS             complement(561..>737)
                     /locus_tag="OQN13_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1870 family protein"
                     /protein_id="HCS1617372.1"
                     /translation="HLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIYQS
                     VAAELYAHDLERLC"
BASE COUNT          239 a          150 c          166 g          182 t
ORIGIN      
        1 actaacaggc taatgattag cggtaataaa aattgtccgc tggaaacaaa tgctttaatt
       61 aaaatattgg ctgtcccagg tgagcgtgga aaagcttcca tcaaactggg ataagtgcct
      121 gcatcgagaa aactgtttgc cattcccgcc agaaagccaa aaacataagc gataatgatg
      181 ttattggtct gcaggatgcc aaaaaagaag gccatatagc agcacatccc gagcatgata
      241 aaagggcggc gaccaaagcg atcggataat aatcctgcaa aaagcaagac acttaatcga
      301 ccaatgccca gcgatgagat aactatcgag acacccgcgg cattagtctg ccaaagtgtc
      361 tccagcgagg ccatattcag gctcatcaaa atgacgccca taccatgcac caggtaatta
      421 aaatacaacc caagtgcggt agggtaatag ggatttttca taggttctac ttagcaaagg
      481 tgctttaaaa agtgtagcga tgttgtgtca gtatccattc attattgttg taacaacgac
      541 aattcctttc tgtggaaaaa ttaacaaagc cgctccagat cgtgtgcata taattcagcg
      601 gctaccgatt gatagatctt ccagtcaata aaattgccat cagggtagac ttgttgaaac
      661 gcggttaagt cggggaaaaa acgcatggtg ttgttgccga tgcggttatt tattttctct
      721 atgatggcat gaagatg
//
LOCUS       DAJVRF010000355          737 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJVRF010000355 DAJVRF010000000
VERSION     DAJVRF010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.360"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..723
                     /locus_tag="OQN13_004196"
     CDS             <1..723
                     /locus_tag="OQN13_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HCS1617373.1"
                     /translation="VGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRD
                     AIHLLTPRAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALG
                     SIAKSGKSAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTP
                     YGLMAVPVIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKK
                     KTFSDQWGLHNQLAVFNPAPVT"
BASE COUNT          170 a          179 c          230 g          158 t
ORIGIN      
        1 gttggtgtgc agtgcggtgg cagcgatgcg ttttctggtg taacggcaaa cccggcggtt
       61 ggctatgcgt ctgatctact ggtgcgctgc ggcgcaacgg tgatgttttc agaagtaacg
      121 gaagtgcgtg acgcgatcca tctgctgaca ccacgcgcag tgaacgaaga ggtcggcaaa
      181 cggctgctgg aggagatgga gtggtacgat aactatctca atatgggaaa aaccgaccgc
      241 agcgccaacc cttcgccggg caacaagaaa ggcggtctgg caaatgtggt ggagaaggca
      301 ctcggctcca ttgctaaatc gggtaaaagt gcaattgttg aagtgctgtc gcccggtcaa
      361 cgcccgacta aacgcggatt aatttacgcc gcgacgccag ccagcgattt tgtctgtggc
      421 acgcaacagg tggcttcggg tatcaccgta caagtgttta cgaccggccg tggtacgccg
      481 tacggcctga tggcggtacc cgtaattaaa atggcaaccc gcaccgagct ggcgaaccgc
      541 tggtttgatt taatggatat taacgcgggc actatcgcta ccggcgaaga gagcattgaa
      601 gaggtgggct ggaagttgtt ccactttatt ctcgacgtcg ccagcgggaa gaagaaaacc
      661 ttctcggatc aatgggggct gcataaccag ctggcggtgt ttaacccggc accggtgacc
      721 tgatttcttt tggaacg
//
LOCUS       DAJVRF010000356          733 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJVRF010000356 DAJVRF010000000
VERSION     DAJVRF010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.361"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..652
                     /locus_tag="OQN13_004197"
     CDS             <1..652
                     /locus_tag="OQN13_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617374.1"
                     /translation="QKNMNTEVLNNRTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQ
                     IQTVGVNLVETVGSNQIIKVGSNQVEKVGIIRALTVGVAYQTTVGGIMNTSVALLQSS
                     QVGLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTGQTAVYSAGEHLELCCGKARLVLT
                     KDGSIFLNGTHIHLEGESDVNGDAPVINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
BASE COUNT          217 a          153 c          213 g          150 t
ORIGIN      
        1 gcagaagaac atgaacaccg aggtgctgaa taaccgcacc acggatgtga taaacaacca
       61 tgccgaaaaa ataggtaaca accaggcgat caccgttacc aataaccaga tccagaacat
      121 tggcgttaat cagatacaga cggttggtgt caacctggtg gaaacggtgg gcagtaacca
      181 gattatcaaa gtgggatcaa accaggttga aaaggtgggg atcattcgtg cgctgacggt
      241 gggtgtggct taccagacga cggtaggcgg cattatgaat acctcggtgg cgttgttgca
      301 gtcctcacag gtagggctgc ataaatcact gatggtggga atgggctaca gcgtcaatgt
      361 agggaataac gtcaccttct cggtgggcaa gacgatgaag gaaaacaccg gacaaacagc
      421 agtttattct gccggtgagc atcttgaact ctgctgtggt aaggcaaggc tggtgctgac
      481 gaaggacgga agcatatttc tcaacggtac gcacattcat ctggaagggg agtcggatgt
      541 gaacggtgat gcgccagtga ttaactggaa ctgtggtgcc acacaacctg taccggatgc
      601 gcctgtgccg aaagatttac cccccggaat gccggatatg cggcaatttt gagtcaggtg
      661 caaaaaatta aaacggaact ggtgcagtga ccagatgaac catattaaaa gcagaaggaa
      721 ttgattcatg ggc
//
LOCUS       DAJVRF010000357          732 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJVRF010000357 DAJVRF010000000
VERSION     DAJVRF010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.362"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            1..732
                     /locus_tag="OQN13_004198"
                     /pseudo
     CDS             1..732
                     /locus_tag="OQN13_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          187 a          184 c          174 g          187 t
ORIGIN      
        1 ttaactatgt tcagtccctg cgcgagttgt acaccgatgt catcgaagag ttttactggg
       61 ttgcgctccc gcttacgacg caaaattccc tttcacaata ccaacccgaa tggcagtgct
      121 gggaacctga tgtcgaatgg gtgcgtcagc ccccgcaaga tgcgataacc gatcctgact
      181 ttttctgctt ttaccagcca ggcatgacct tcgaacaatt tgtacgtgag tttgccgaat
      241 ggttttcaca aaaacgtccg gtggcgatga tgatcggcat ccgtgcggat gagtcctaca
      301 accgttttgt cgccatcgcc agtttaaata aacaacgttt tgccgacgat aaaccatgga
      361 ctacggccgc accaggcggt catagctggt acatttaccc catttacgac tggaaagtgg
      421 ctgatatctg gacctggtat gcaaatcatc aaaatctctg caacccactg tataacctaa
      481 tgtatcaggc aggcgttcct ctgcgtcata tgcgaatttg agaacctttt ggcccggagc
      541 aacgacaagg attatggctc tatcacgtta tcgaaccgga tcgctgggct gctatgtgcg
      601 cacgagtcag cggggtaaaa agtggcggca tttacgccgg acatgataat catttctatg
      661 ggcatcggaa aatcctcaag ccagaacatt tagactggca agaatatgca ttattgctgc
      721 tcaatagcat gc
//
LOCUS       DAJVRF010000358          730 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJVRF010000358 DAJVRF010000000
VERSION     DAJVRF010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.363"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(1..557)
                     /locus_tag="OQN13_004199"
                     /pseudo
     CDS             complement(1..557)
                     /locus_tag="OQN13_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
BASE COUNT          180 a          183 c          183 g          184 t
ORIGIN      
        1 ccagtgaatc ccatcaccag gaaaacgaag ccgtttttgg tgatttggta catcgggcgt
       61 ttttcgcctt tggcgtcagt gtaggtgacg tcctcaaaat tgagggcgtt aaattcaggt
      121 gagcattcag tatttgcgat ggcacgtaac acattgtcgt gacgcttgcc aaagaactct
      181 gcgatcgcaa tagacgtagt gacagcgcga ccattttcga tggttacgtc agggtgagaa
      241 agggcaggga tagtagccat gatggcatcc tcgagtgata agttaattaa ctcaccaccg
      301 aggttttcca cgaccataag ggtggtgaga cgtacagggg tggaaatacc ggtcactcga
      361 gaacccggcc agtcttgcga ctgccctgca catcccacca taatttgaat ggagctgtgc
      421 attacgcata aaaaaaccgc ttcagcgcgg ctatgcgctc aagtaacgtt cgggtttcca
      481 cgcccggcac ccgttttata aggtgcaggt gcactataat tccacccgtt ctggttttca
      541 atagctacat tcaacatttt ctctttcctt tcatcaccga agtgaacttt gttgatgcgg
      601 tgcctggtgc ctccaggtga cgttaaccag ttaacaatta acgccggata cagggaaacc
      661 cataaccacc cgatacgggg gaactaatcg ctttttaact gtgccgcgtg cgctgagccg
      721 cattcaccgc
//
LOCUS       DAJVRF010000359          727 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJVRF010000359 DAJVRF010000000
VERSION     DAJVRF010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.364"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(158..>727)
                     /locus_tag="OQN13_004200"
     CDS             complement(158..>727)
                     /locus_tag="OQN13_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1617375.1"
                     /translation="FFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIV
                     IAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFF
                     IAVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQ
                     IHLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          226 a          179 c          157 g          165 t
ORIGIN      
        1 tggcttgata accggataac aacgtgcgct ttgttgatgc cggatgcggc gtgaattcct
       61 tatccggtct acaaaatttg caaatccaaa gcattacatt aagacgtggc ctgataaaca
      121 tatcgcatca ggccattttc gttagctatt gtccacgtca ggctttcaca tcactcactg
      181 caccgaccgg aatatcagca tgaacaggct cgggcggtac tttgcgatat ttatccagca
      241 gatgaatctg gatcctgcgc agcgtgtcgc cattgaggcg atagaaacgg aagtagagaa
      301 tcagcgttac catgaaaaac agagttggta gagcaatcat aataaactgc atacctaata
      361 gggcttgcgt agactgttca acattcggta catagccaat catccctaac acaaccgcaa
      421 tgaaaaaagc cgcaaaggct gaaccgccct tcaccaccat agtctgcacg gagtaagcga
      481 tactttcaca gcgtacgtgc agtttatatt caccgtaatc aacggtatct gccaccatga
      541 tgacctgtaa tacccagaaa agcgccgttc ccacattcag caaaataccc gcaatcacaa
      601 tgaggacgac gttgtgatag ctcattaatg ccattaacag gagaacacca cagcttaaca
      661 ccggaagaat agatgctccg gcccataaaa tgcgtcggga taatgattta actaagcgtg
      721 ggaagaa
//
LOCUS       DAJVRF010000360          726 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJVRF010000360 DAJVRF010000000
VERSION     DAJVRF010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.365"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            35..726
                     /locus_tag="OQN13_004201"
                     /pseudo
     CDS             35..726
                     /locus_tag="OQN13_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
BASE COUNT          167 a          183 c          205 g          171 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 tgcacgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      361 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      421 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      481 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      541 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      601 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      661 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAJVRF010000361          721 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJVRF010000361 DAJVRF010000000
VERSION     DAJVRF010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.366"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..>721)
                     /locus_tag="OQN13_004202"
     CDS             complement(<1..>721)
                     /locus_tag="OQN13_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCS1617376.1"
                     /translation="KAIMVNGPQFGWYAPAYTYGIGLHGAGYDVTGNTPFAYPGLVFG
                     HNGVISWGSTAGFGDDVDIFAERLLAEKPGYYLHNGKWVKMLSREETITVKNGQAETF
                     TVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAKNWQEWTQQAAKQA
                     LTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKGLLPFEMNPKVYNP
                     LSGYIANWNNSPQKDYPASDL"
BASE COUNT          188 a          193 c          153 g          187 t
ORIGIN      
        1 aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc aatatatccc
       61 gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt ccagtcccat
      121 tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc tggataagca
      181 ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt gatggtcagt
      241 gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat ctgatgagtc
      301 caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc gtaagccgtt
      361 tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac agtaaaggtc
      421 tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt cacccactta
      481 ccattatgca agtagtagcc tggtttctct gccaacagcc gttcagcaaa aatatcgaca
      541 tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc aaaaaccagc
      601 ccaggatagg caaatggtgt attgccagtg acatcataac cagcaccgtg cagaccaata
      661 ccataagtat acgcaggcgc ataccagcca aactgcggac catttaccat gattgctttc
      721 g
//
LOCUS       DAJVRF010000362          717 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJVRF010000362 DAJVRF010000000
VERSION     DAJVRF010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.367"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..236
                     /locus_tag="OQN13_004203"
     CDS             <1..236
                     /locus_tag="OQN13_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617377.1"
                     /translation="LFRTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGESGLKW
                     EAKDSSKLIGNKDHALRGLSSPIVGIRQIRLIT"
     gene            complement(253..>717)
                     /locus_tag="OQN13_004204"
     CDS             complement(253..>717)
                     /locus_tag="OQN13_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCS1617378.1"
                     /translation="LGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQSTPQM
                     STLQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAWAGIVL
                     ASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYEGVTQR"
BASE COUNT          224 a          172 c          169 g          152 t
ORIGIN      
        1 aacttttcag aactcttctg aatcagatcc ccttattatc atccctgcaa agtggaacat
       61 tgccattgtt tggctattca ggacgggtca gaccaatgaa aaaagcccac acaggggaga
      121 gtgggctgaa atgggaagct aaagactcaa gtaaacttat cggaaataag gaccacgcat
      181 tacgggggct atcatcgccg atagtcggga ttcggcaaat caggttgatt acgtagagag
      241 taaattatct gctcaccgct gcgtcacccc ttcgtaaatc aggcgtagcg cgaatacacc
      301 aataattgca ccaataaccc gactggcaac gcgttgcata cgcccataag cacgacgcac
      361 agcgggcaaa gaaaacgcct gactaagaaa gactcgccag ataactgatg cgagcacaat
      421 ccccgcccag gccattaaac gtgcccatgt tggtgtttcg gcatttaatg ttactgagaa
      481 aatactgata aaaaataaaa cggtttgcgg gttagagaga tcggtaatta atccgcggcg
      541 aaaaaagaca taccaggagg cgctaatcgg ttgttgtagt gtgctcattt gcggtgttga
      601 ctggcggcgc atgctgcacc acgcaaacca taagagataa gcgccgccga cgattctgat
      661 aagcgaaaaa atctcctcac actgcgttat tagcgttgca agaccaaaca aacccaa
//
LOCUS       DAJVRF010000363          713 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJVRF010000363 DAJVRF010000000
VERSION     DAJVRF010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.368"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..134)
                     /locus_tag="OQN13_004205"
     CDS             complement(<1..134)
                     /locus_tag="OQN13_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCS1617379.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     T"
     gene            complement(336..>713)
                     /locus_tag="OQN13_004206"
     CDS             complement(336..>713)
                     /locus_tag="OQN13_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCS1617380.1"
                     /translation="DNAIAYKEQRDNKASELEKANATIADMRKRQRDVAELDARYIKE
                     LADANATIESLRADVSAGRKRLQVAATCAKSTTGASGMGDGESPGLTADAELNYYRLR
                     SGIDKITAQVNYLQEYIRTQCLK"
BASE COUNT          155 a          180 c          175 g          203 t
ORIGIN      
        1 gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt cacttttacc actggctttc
       61 cacatatcag tgaggcagat catgccatct tcaccgatac gaattggttg attgaagagg
      121 gttaatgatt tcatagcgtg tacctactct ttgaaatgaa cctttgccgc acaggaaacc
      181 agcccaccga ggctcgccag cactaactgg tatcctcaaa ggcccattcc aaaggggcag
      241 gttcggtgta aaaaacatgc gttgcggtac gcatttattg caaaaagccc cgcatcgcga
      301 ggctcattaa atggactttg tgatttgcaa aaaaattatt tcaggcattg cgtcctgatg
      361 tattcctgca ggtagttaac ctgcgcggtt atcttgtcga ttccacttcg gagacggtaa
      421 taattgagtt cagcatctgc tgtaagtcct gggctttctc catcgcccat gccgctggct
      481 ccggtcgttg actttgcaca ggtggcggcg acttgcaggc gcttacgccc agcagaaaca
      541 tcagcacgga gactttcgat agtcgcgtta gcatcagcaa gctcctttat gtatcttgcg
      601 tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag cgatggtggc gttcgccttc
      661 tccagttcac tggccttgtt atcgcgctgc tctttgtagg cgattgcgtt atc
//
LOCUS       DAJVRF010000364          700 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJVRF010000364 DAJVRF010000000
VERSION     DAJVRF010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.369"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
BASE COUNT          242 a          108 c          143 g          207 t
ORIGIN      
        1 gatcgattca gatgccattt tgcagtagtc tattttataa aaaaattaag taggctaaga
       61 agtaccttag aaatcaattt taaggggtct aaaacgctct gtaacgagct aaactgtata
      121 agtggcagta agatgccttt tagactgaca ctcgcttaga gagcttccta gagcttctgg
      181 gaggattcat tagatatcgt tgtatacaga gacacagtat ctataaaggg tattaagaga
      241 ttcttagaag ttactgtaaa gaaccactaa gataagaaga gagggacaaa gaaccgaagg
      301 aaagtccctt attaagtagg agtcttaata gactgtctta atagataact taaaagtctt
      361 aaaggatatc ttccaagaag agaggaacga agaaccagag gaagtgaccg ttgaccgttt
      421 gaacagactg tttaagtaaa gcctataaag tatctattaa ttaagggcta tgtctcaata
      481 ctagcagttt gtcaatacct ttgtctttag caattcgtgc tatttattaa atatagcact
      541 ttttgttaaa acagagtatg ctttttaacc taaaaccttg tcgcttcgct ctgggtcaca
      601 tgttcttggg ttcttagtga gagttacact atcttctaag gtactcaata aaaagacgag
      661 taacaaccga agaacgagga tagaggtatt gtaataactt
//
LOCUS       DAJVRF010000365          691 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJVRF010000365 DAJVRF010000000
VERSION     DAJVRF010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.370"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>691
                     /locus_tag="OQN13_004207"
     CDS             <1..>691
                     /locus_tag="OQN13_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extended Signal peptide of Type V secretion
                     system family protein"
                     /protein_id="HCS1617381.1"
                     /translation="YQANSNGKVIVEKGGEWLIKNNDSSIEFQIGNQGAGEATIREGG
                     LITAENTIIGGNATGIGTLNVQDQDSVITVRRLYNGYFGNGTVNISNNGLINNKEYSL
                     VGVQDGSHGVVNVTDKGHWSFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIITAGM
                     KETGTGNITVKDKNSVITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGENGVGNV
                     SITTGGMWEVNK"
BASE COUNT          203 a          123 c          196 g          169 t
ORIGIN      
        1 atatcaagcg aacagtaatg gtaaggttat cgttgaaaag ggtggcgagt ggctaataaa
       61 aaataatgat tcctcaattg aatttcaaat tggtaatcaa ggagctgggg aggcgactat
      121 tcgcgagggt gggctgatta cggctgaaaa tacgattatc ggtggcaatg ccaccggtat
      181 cggaaccctg aatgtgcagg atcaagactc tgtcatcacg gtacgcagac tctataatgg
      241 gtatttcggt aatggcacag tcaatatttc caataatggt ctgattaata acaaagaata
      301 ttcattggtg ggcgttcagg acggttccca cggtgtcgtc aacgtgaccg ataaagggca
      361 ttggagtttc ctcggaacag gcgaagcttt ccgctatatc tatatcggtg atgctggtga
      421 cggtgaactt aatgtctcgc gtgaaggtaa agtagattcg ggaattatca ctgcggggat
      481 gaaagaaaca ggcacaggca acattactgt taaggataag aactccgtta tcactaatct
      541 cggaactaat cttggttatg acggccacgg cgaaatgaat atcagtaatg aggggcttgt
      601 tgtcagcaac ggaggaagct cactcggtta tggagaaaac ggcgtcggga atgtcagcat
      661 caccacgggg gggatgtggg aggtcaataa g
//
LOCUS       DAJVRF010000366          688 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJVRF010000366 DAJVRF010000000
VERSION     DAJVRF010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.371"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..543
                     /gene="ipaH9.8"
                     /locus_tag="OQN13_004208"
     CDS             <1..543
                     /gene="ipaH9.8"
                     /locus_tag="OQN13_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCS1617382.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRT
                     EADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQ
                     LTDEVLALRLSENGSQLHHS"
BASE COUNT          168 a          188 c          200 g          132 t
ORIGIN      
        1 accggcgctc tgctctccct gggcagggaa atgttccgcc tcgaaattct ggaggacatt
       61 gcccgggata aagtcagaac tctccatttt gtggatgaga tagaagtcta cctggccttc
      121 cagaccatgc tcgcagagaa acttcagctc tccactgccg tgaaggaaat gcgtttctat
      181 ggcgtgtcgg gagtgacagc aaatgacctc cgcactgccg aagccatggt cagaagccgt
      241 gaagagaatg aatttacgga ctggttctcc ctctggggac catggcatgc tgtactgaag
      301 cgtacggaag ctgaccgctg ggcgctggca gaagagcaga aatatgagat gctggagaat
      361 gagtaccctc agagggtggc tgaccggctg aaagcatcag gtctgagcgg tgatgcggat
      421 gcggagaggg aagccggtgc acaggtgatg cgtgagactg aacagcagat ttaccgtcag
      481 ctgactgacg aggtactggc cctgcgattg tctgaaaacg gctcacaact gcaccattca
      541 taatcacatc gcataaacca cagaccggac tgactccgga aaaacagagg cccgcccccg
      601 ggcctccccg gattcatccg tttccctgtt cagcctgaca gcacgccccc gcggccggat
      661 gacagactcc gcttcggtaa gcaaagcg
//
LOCUS       DAJVRF010000367          686 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJVRF010000367 DAJVRF010000000
VERSION     DAJVRF010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.372"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>686
                     /locus_tag="OQN13_004209"
     CDS             <1..>686
                     /locus_tag="OQN13_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1617383.1"
                     /translation="DISHLSEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHET
                     RHAYNAQGLANRCIPDSLSAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETLRSFG
                     RYELTIAYTPAGQLQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLTG
                     VHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDHHGRL
                     TEKTDLIPEGV"
BASE COUNT          157 a          231 c          199 g           99 t
ORIGIN      
        1 gacatcagcc atctcagcga agggcaccgg gtgacggtgc attacgggta tgatgagaaa
       61 ggccggctga ccggtgagcg tcagacggtg catcacccgc agacggaagc actgctctgg
      121 cagcatgaga ccagacatgc gtacaacgcg caggggctgg cgaaccgctg tataccggac
      181 agcctgtccg ccgtggaatg gctgacctac ggcagcggct ggctgtcagg catgaaactc
      241 ggcgacacac cgctggtgga gtacacccgc gaccgcctgc accgggaaac gctgcgcagc
      301 ttcggccgtt atgaactcac catcgcttat acccctgccg ggcagttaca gagccagcac
      361 ctgaacagcc tgctgtctga ccgcgattac acctggaacg acaacggcga actcatccgc
      421 atcagcagcc cgcgccagac ccggagttac agctacagca ccaccggcag gctgaccggc
      481 gttcacacca ccgcagcgaa tctggatatc cgcatcccgt atgccacaga cccggcgggg
      541 aaccgcctgc ccgacccgga gctgcacccg gacagcaccc tcagcatgtg gccggataac
      601 cgtatcgccc gtgacgcgca ctatctttac cggtatgacc atcacggcag actgacagag
      661 aaaaccgacc tcatcccgga aggggt
//
LOCUS       DAJVRF010000368          685 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJVRF010000368 DAJVRF010000000
VERSION     DAJVRF010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.373"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..393)
                     /locus_tag="OQN13_004210"
     CDS             complement(<1..393)
                     /locus_tag="OQN13_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCS1617384.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQ"
     gene            complement(432..>685)
                     /locus_tag="OQN13_004211"
     CDS             complement(432..>685)
                     /locus_tag="OQN13_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617385.1"
                     /translation="NGLYAVRVENGAIVSHCRIRCLQSQQRKSGAVLFYFCNGLLTDG
                     FILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
BASE COUNT          187 a          177 c          150 g          171 t
ORIGIN      
        1 ctgaatggtt acccactcgc gaaaagcctc aaacgacttt agcaatgccg tatcccgggt
       61 tctgcgtgtc gcaactgtat tcagatattg ctctgcggct tcgctcagag ctggcgtatg
      121 ttcccgacct actgattcgc acaggtaatc aacgaaacca gacagcagtt ctcgttcgcg
      181 aggcgtgatc gccccaccga ccggagtcca gtaatcgaat cccagttgca ggagtttgaa
      241 aaaacgcttg tggaatgcgt agttacgcac acgcttaaag tctgcgtgta tccactcacc
      301 tgttttgatt tgatgcagaa aatcgcaact ctccggcgtc gccgggagaa gtaatccgga
      361 agaggtttgt ttgaccagtt gtatatgtgc catcggcttt ctccggtggc acggtgttac
      421 acagcaggag ttcaatcctg ctcaagattg tagatgagtt tattcttcag caaaagcaga
      481 aaaccagcct taaaaccaat ctctttcaaa acccgtaatg atgtgacaaa ttcgtcctca
      541 cgcaaaataa aaccgtccgt cagaagtcca ttacaaaaat aaaataacac agcaccgctc
      601 ttcctttgtt gagattgcaa acatctaatg cggcaatggc tgacaatcgc tccattctca
      661 acgcgcacag catagaggcc atttt
//
LOCUS       DAJVRF010000369          682 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJVRF010000369 DAJVRF010000000
VERSION     DAJVRF010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.374"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            242..583
                     /gene="raiA"
                     /locus_tag="OQN13_004212"
     CDS             242..583
                     /gene="raiA"
                     /locus_tag="OQN13_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCS1617386.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
BASE COUNT          224 a          151 c          141 g          166 t
ORIGIN      
        1 ttgagctgcc gtttttttat tctgttagtt gtgaaactga agcgatttag tcactatcga
       61 tctcatcaaa tatggctcgc tttgagatat tcctcaagta aaaaaacacc tcttcctgcg
      121 atttctcaca aaaaagattc gttgacaaaa agtgacaaaa ttatgagatt cccatcacac
      181 attttgacat caggaacggt atgctgaatt caccaagacg ggaagacaag aggtaaaatt
      241 tatgacaatg aacattacca gcaaacaaat ggaaattact ccggccatcc gccaacatgt
      301 cgcagaccgt ctcgccaaac tggaaaaatg gcaaacacat ctgattaatc cacatatcat
      361 tctgtccaaa gagccacaag ggtttgttgc tgacgccaca atcaatacac ctaacggcgt
      421 tctggttgcc agtggtaaac atgaagatat gtacactgca attaacgaat tgatcaacaa
      481 gctggaacgg cagctcaata aactgcagca caaaggcgaa gcacgtcgtg ccgcaacatc
      541 ggtgaaagac gccaacttcg tcgaagaagt tgaagaagag tagtccttta tattgagtgg
      601 atcgccaacg cgccttcggg cgcgtttttt gttgacagcg tgaaaacagt acgggtactg
      661 tactaaagtc acttaaggaa ac
//
LOCUS       DAJVRF010000370          679 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJVRF010000370 DAJVRF010000000
VERSION     DAJVRF010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.375"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>679
                     /locus_tag="OQN13_004213"
     CDS             <1..>679
                     /locus_tag="OQN13_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1617387.1"
                     /translation="AAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSATGP
                     DGLEMRRKYDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQ
                     LLTVTDCSGYVTRYDHDRFGQVTAVHREEGLSQYRAYDSRGQLIAVKDTQGHEMRYEY
                     NAAGDLTAVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLTSENGSHT
                     TFRYDVLD"
BASE COUNT          166 a          202 c          197 g          114 t
ORIGIN      
        1 gccgcaggcc ggacaacaga atacagcccg gatgtggtga cgggcctcat cacgcgcatc
       61 accacgccgg atggcagggc atcggcgttt tactataacc accacagcca gttaacgtca
      121 gccaccgggc ctgacgggct ggaaatgcgc cgcaaatatg atgaatccgg ccgtctgatt
      181 caggaaaccg cccctgacgg cgatatcacc cgctaccgtt atgataatcc acacagtgac
      241 ttaccctgcg caacggaaga tgccaccggc agccggaaaa ccatgacatg gagccgttac
      301 ggtcagttgc tgaccgtcac tgactgttcc ggttatgtaa cccgttatga ccatgaccgt
      361 tttggtcagg tgacggcggt gcaccgcgag gaagggctga gccagtaccg cgcatacgac
      421 agccgtggac agttaattgc ggtgaaagac acgcagggcc atgaaatgcg gtatgaatac
      481 aacgccgccg gtgacctgac cgccgtcatt gccccggacg gcagcagaaa cgggacacag
      541 tacgatgcgt ggggaaaagc catctgtacc acgcagggcg ggctgacgcg cagtatggaa
      601 tacgatgctg ccggacgggt catccgtctg accagtgaaa acggcagcca caccaccttc
      661 cgttacgatg tactcgacc
//
LOCUS       DAJVRF010000371          670 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJVRF010000371 DAJVRF010000000
VERSION     DAJVRF010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.376"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            364..471
                     /locus_tag="OQN13_004214"
     CDS             364..471
                     /locus_tag="OQN13_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCS1617388.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT          181 a          156 c          200 g          133 t
ORIGIN      
        1 gcgtcaggct gccgtaatgg caatttgcgc ccggaccagg ccgcaggagt gaaactctgc
       61 ggcctttttc gtaagaaaaa tgcaacggtt gcaagcggat aatattctgc ggaatgcgca
      121 gcacagagtg aagatgagac ggattccgga acagaaaaac agtcgtagaa tcagcgtcaa
      181 gccggaagga ttccggtgag gcgcaatgtt gcgggggctt aatccctggt ggcattggtt
      241 gctggagaga gaaaaccccc gcacgttgca ggtatgcacc tgacaacacc acgggggcca
      301 ttcttgcatc caaacaatac aagaatagcc gcgaaacgtt gtcattacaa tacaggcggc
      361 tatatgacgc tcgcagaact gggcatggcc ttctggcatg atttagcggc tccggtcatt
      421 gctggtattc ttgccagtat gatcgtgaac tggctgaaca agcggaagta acgtgccatg
      481 cgggcgtcag gctgccgtaa tggcaatttg cgcccggatc aggccgcagg ggtgaaactc
      541 tgcggccttt ttcgtaagaa aaatgcaacg gttgcaagcg gataatattc tgcggaatgc
      601 gcagcacaga gtgaagacgt gacggattcc ggaacagaaa aacagtcgta gaatcagcgt
      661 caagccggaa
//
LOCUS       DAJVRF010000372          669 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJVRF010000372 DAJVRF010000000
VERSION     DAJVRF010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.377"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..575)
                     /gene="nagD"
                     /locus_tag="OQN13_004215"
     CDS             complement(<1..575)
                     /gene="nagD"
                     /locus_tag="OQN13_004215"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCS1617389.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQA"
BASE COUNT          171 a          183 c          166 g          149 t
ORIGIN      
        1 tgcgcctgca ttttgtttaa tgctgcgcgg atgatccacg ggctgggctt accaacatag
       61 aacggtttgc gcccggagat tttctcaatc cctgcacaca acgcgccaca agcgggataa
      121 aaaccgcgcc cgtgggtgtc cgggttggtg gcgataaagc gtgcaccgtt agcgacgaaa
      181 taggccgctt tatgcatcat gtcccagttg taggaacgcg tttcgccaac aatcacgaaa
      241 tcagggttca catcggtaat agtgaaaccg gctttgtaca gttcatgaat cagtgcgcct
      301 tcgcccacca catatgcttt cttgccttcc tggcgacgca ggaaatcggc ggtcgccatc
      361 gcagaggtat aaaacacgct atccggcaca tcgacaccgg cagtggcaaa gcggttcgcc
      421 agatcttgcc cggtctgcga aggatagttg gtcagcaaca ccagcggcag gcctttatcc
      481 ataatcccgt gcaaaaattc cgctgcaccc ggtacggcga cgttatcgtg catcagcacg
      541 ccgtcgatat cgcaaattac atttttaatg gtcatggact acccagaata ttgacaacaa
      601 taagcgccac tataaaagca cattaatttt ccagcaaatg ctggagcaaa ataccgttga
      661 gcatggcgc
//
LOCUS       DAJVRF010000373          664 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJVRF010000373 DAJVRF010000000
VERSION     DAJVRF010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.378"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(1..425)
                     /locus_tag="OQN13_004216"
                     /pseudo
     CDS             complement(1..425)
                     /locus_tag="OQN13_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          204 a          141 c          118 g          201 t
ORIGIN      
        1 ctgcccgaga atcagactgt gcattgttga gaacgcacta atgacatgaa ttgctcccct
       61 gcggcactct tgtcataaga gtgccggagc gtttttccat caattgcaat gacgtcttta
      121 tcatctgaag aatggcagtc acgcatccag ttaataaagc actcgtgaaa ttttgcagga
      181 ctgatacagg atacaactct ggcgatggta tcgtgaacag gaataccatt ttcaaaatca
      241 ccatattgtt ttaaaaaatc gagatgtgtt tccccaaaat cctctatatc ttcccagcct
      301 tctgcaccag aaataacggc acaaatagtc aacagtagaa tatccgataa tttatgttcc
      361 actttccagg cttgttttta atcggggata atagaaatat gttccatcaa ttttttaagt
      421 tccattttgt tctccttaat tatgtaagga gtatttgatc atgtataagc aataaaaaac
      481 agcttcaggt aataaggaat atctcaattt ttaaacataa aatgcgaatt atttagtaca
      541 aaaagtgaac tcctcagggc aagattacga aagtccgctc cccgcaagga ctgacgcgag
      601 atagtttctg tccatggctg cttttcgcat cttacgtctt aaccctgcct tgaatacctt
      661 atca
//
LOCUS       DAJVRF010000374          654 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJVRF010000374 DAJVRF010000000
VERSION     DAJVRF010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.379"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..419
                     /locus_tag="OQN13_004217"
     CDS             <1..419
                     /locus_tag="OQN13_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1617390.1"
                     /translation="FASDTIKMVYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRR
                     ITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDREKLK
                     AYRIYAKSLQAMDFSTITDKSSYNAIEWSVSPEGSS"
     gene            443..>654
                     /locus_tag="OQN13_004218"
     CDS             443..>654
                     /locus_tag="OQN13_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCS1617391.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEY"
BASE COUNT          170 a          147 c          182 g          155 t
ORIGIN      
        1 actttgccag tgacaccata aaaatggttt acaccggaga cgggcgcgtg gtgtgggtcg
       61 gtaaggatgt gacaggcatt gaaccccgta acgccagtgt tattgaagtt cctgatatta
      121 ccgccaaccg ccgcattacc gcgccgggtt actggtttta ccgcaatgat gaatttgtct
      181 ttgactacag actcaaagcg gaagatgagc gtgatgccct tctggctcag gtcagtgccc
      241 ggacagggga atgggaagaa gacctgctgc tggggctaat cagcgacgaa gaccgggaaa
      301 aactgaaagc gtaccgtatt tacgcgaaat cgctgcaggc gatggatttc agcaccatca
      361 ctgataaatc ctcatataac gccattgaat ggtccgtctc tccggaaggt tcttcctgat
      421 ttaatttatc gcgagaaaaa ctatgtctgt agtgatatca ggtgcgctga ctgatggcgc
      481 aggcatcccc atgtccggat gccagattat tctgaaatcc cgtgtaaaca cctcagaagt
      541 ggtgatgcgt accagggctg atgtagtgac cggaaataac ggtgaatatt cgtttgaggc
      601 acaggtcgga aaatattgcg tgtatctgaa acgggactgg cgcgacgagt actg
//
LOCUS       DAJVRF010000375          653 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJVRF010000375 DAJVRF010000000
VERSION     DAJVRF010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.380"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>653
                     /locus_tag="OQN13_004219"
     CDS             <1..>653
                     /locus_tag="OQN13_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1617392.1"
                     /translation="IPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIWGIILGRA
                     LQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITHKLGLHAL
                     FWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLNFGIMCLH
                     MLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVERKMKQVFV"
BASE COUNT          122 a          171 c          182 g          178 t
ORIGIN      
        1 gattccgttt ggcctgcttt ccgaccgcat tggtcgcaaa ccattaattg tcggtgggct
       61 ggcggtgttt gccgccggta gcgttatcgc tgcgctttcc gactccatct ggggaattat
      121 tctgggccgg gcgctacaag gctctggagc aattgccgcc gccgttatgg cgctgctttc
      181 cgatctcacg cgcgaacaaa accgcaccaa agcgatggcg tttatcggcg tgagctttgg
      241 cattaccttt gccattgcga tggtgcttgg cccgatcatc actcacaaac ttgggctgca
      301 cgcgctgttc tggatgatcg ctattctggc aacaaccggc attgcgttga ccatttgggt
      361 tgtgcccaac agtagcactc acgtacttaa tcgtgagtcc ggaatggtga aaggcagttt
      421 cagtaaagtg ctggcggaac cgcggctgct gaaactcaac tttggcatta tgtgtctgca
      481 catgcttctg atgtctacgt ttgttgccct gcccggacag ctagctgatg cggggttccc
      541 ggcggctgaa cactggaagg tctatctggc gacgatgtta atcgcctttg gctcgatcgt
      601 gcctttcatt atctacgctg aagttgagcg caaaatgaag caagtctttg tct
//
LOCUS       DAJVRF010000376          649 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJVRF010000376 DAJVRF010000000
VERSION     DAJVRF010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.381"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            1..231
                     /locus_tag="OQN13_004220"
                     /pseudo
     CDS             1..231
                     /locus_tag="OQN13_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
BASE COUNT          208 a          108 c          150 g          183 t
ORIGIN      
        1 gccactaatg gtacaaactc tgactccgac ctgaattcaa ttcaggatga aattacacaa
       61 cgccttagtg aaattgatcg tgtttctaac cagacacaat ttaatggtgt aaaagttctg
      121 gcttctgatc agactatgaa aattcaagta ggtgcgaacg atggtgaaac cattgagatt
      181 gcccttgata aaattgatgc taaaaccttg gggcttgata actttagcgt agcactagga
      241 aaagttccaa tgtcctctgc ggttgcactt aagagcgaag ccgctcctga cttaactaag
      301 gtaaatgcaa ctgatggtag tgtgggaggt gctaaagcat tcggtagcaa ttataaaaat
      361 gctgatgttg aaacttattt tggtaccggt aatgtacaag atacaaagga tacaactgat
      421 gcgaccggta ctgcaggaac aaaagtttat caagtacagg tggaagggca gacttatttt
      481 gttggtcaag ataataatac caacacgaac ggttttacat tattgaaaca aaactctaca
      541 ggttatgaaa aagttcaggt gggtggtaag gatgttcagt tagcaaactt tggtggtcgt
      601 gtaactgcat ttgttgaaga taatggttct gccacatcag ttgatttag
//
LOCUS       DAJVRF010000377          646 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJVRF010000377 DAJVRF010000000
VERSION     DAJVRF010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.382"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..333
                     /gene="aroD"
                     /locus_tag="OQN13_004221"
     CDS             <1..333
                     /gene="aroD"
                     /locus_tag="OQN13_004221"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCS1617393.1"
                     /translation="HDFHKTPEAEEIIARLRKMQSFDADIPKIALMPQSTSDVLTLLT
                     ATLEMQEQYADRPIITMSMAKTGVISRLAGEVFGSAATFGAVKKASAPGQISVNDLRT
                     VLTILHQA"
     gene            477..>646
                     /locus_tag="OQN13_004222"
     CDS             477..>646
                     /locus_tag="OQN13_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617394.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQT"
BASE COUNT          185 a          153 c          148 g          160 t
ORIGIN      
        1 catgacttcc ataaaacgcc ggaagccgaa gaaatcattg cccgtctgcg caaaatgcag
       61 tccttcgacg ccgatattcc taagattgcg ctgatgccgc aaagtaccag cgatgtgctg
      121 acgttgctta ccgcgaccct ggagatgcag gagcagtatg ccgatcgtcc aatcatcacg
      181 atgtcgatgg caaaaactgg cgtaatttct cgtctggctg gtgaagtatt tggctcggcg
      241 gcaacttttg gtgcggtaaa aaaagcctct gcaccagggc aaatctcggt aaatgattta
      301 cgcacggtat taactatttt acaccaggca taagcaataa tatttcggcg ggaacaccct
      361 ccccgccgaa cttaaaaaat atatttaacc gtatttaata aaaatatttc gtgagtctct
      421 gtgcgctaat tctccatctg gcgtagggaa aatcacatct gaatcaggaa ttaacaatga
      481 aacctgtaaa accacctcgt attaatggac gagtgccggt cctgtcggca caggaagcgg
      541 tgaattatat tcccgacgaa gcaacacttt gtgtgttagg cgctggcggc ggtattctgg
      601 aagccaccac gttaattaca gctcttgctg ataaatataa acagac
//
LOCUS       DAJVRF010000378          640 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJVRF010000378 DAJVRF010000000
VERSION     DAJVRF010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.383"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..618
                     /locus_tag="OQN13_004223"
     CDS             <1..618
                     /locus_tag="OQN13_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HCS1617395.1"
                     /translation="RLRKELRLRCKQKRKFRATTNSNHNLPVAPNLLNQTFAPTAPNQ
                     VWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPA
                     GLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYR
                     FNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          180 a          158 c          164 g          138 t
ORIGIN      
        1 cgtcttcgta aggagctaag gctacgctgt aagcagaaac gcaagttcag agcgactacg
       61 aactcgaacc acaatctgcc agttgcgcca aatctgctga accagacgtt cgctcctaca
      121 gcaccaaatc aggtctgggt ggcggacctg acgtatgttg ccacacagga gggatggttg
      181 tacctcgctg gcatcaaaga tgtttatacg tgcgaaattg tcggctacgc catgggagag
      241 cgcatgacaa aagagctgac aggtaaagcc ctgtttatgg cgctcaggag ccagcgccca
      301 cctgccgggc taatccacca ctctgatcga ggttcacagt actgcgcata cgattaccgg
      361 gtcatacagg agcagtctgg tctgaaaaca tcaatgtcgc gtaaaggtaa ctgttacgac
      421 aacgctccga tggaaagctt ctggggaacg ctgaaaaatg agagcctgag ccactatcgt
      481 tttaataacc gggatgaagc catctcagta atacgggaat acattgagat tttctacaat
      541 cgtcagcgtc gtcactctcg tctggggaat atctccccgg cagccttcag ggaaaaatat
      601 catcagatgg ctgcttaaaa aaagaacaaa tggtagtgtc
//
LOCUS       DAJVRF010000379          638 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJVRF010000379 DAJVRF010000000
VERSION     DAJVRF010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.384"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..591
                     /gene="yebU"
                     /locus_tag="OQN13_004224"
     CDS             <1..591
                     /gene="yebU"
                     /locus_tag="OQN13_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="in Escherichia coli this enzyme specifically
                     methylates C1407 of the 16S rRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCS1617396.1"
                     /translation="ALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNF
                     PFSPVKDREAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGI
                     KLAETHNKGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLV
                     TFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGNA"
BASE COUNT          152 a          163 c          172 g          151 t
ORIGIN      
        1 gcgctgaccg aggaaggctt tttgcatgtt ttcccacaaa tttacgactg cgaaggcttc
       61 ttcgttgctc gtctgcgtaa aactcaggcg attcccgcct tacccgcccc caaatacaaa
      121 gtcggtaatt ttccgttcag cccagtgaaa gatcgagaag ctggacaaat tcgtcaggcg
      181 gctgcaggtg ttggcttaaa ctgggatgaa aacctgcgcc tctggcagcg tgacaaagaa
      241 ctgtggttgt tcccggtggg cattgaagcc ctgatcggta aagtccgatt ttctcgcttg
      301 gggattaaac ttgccgaaac gcacaacaaa ggttatcgct ggcagcatga agcagttatt
      361 gcccttgcca cccccgacaa tgtgaacgct tttgaactga caccgcagga agcggaggag
      421 tggtatcgcg ggcgcgatgt ttacccgcaa gccgcgccag tggcggatga cgtgttggtt
      481 actttccagc atcaaccgat tggtttagcc aaacggattg gttcgcgatt gaaaaacagc
      541 tatccgcgtg aactggtgcg cgatgggaaa ctttttaccg gtaacgcctg acagcgcaca
      601 aaaaaagcgc actttttgac tggcacattc ggctgcct
//
LOCUS       DAJVRF010000380          629 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJVRF010000380 DAJVRF010000000
VERSION     DAJVRF010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.385"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(38..629)
                     /locus_tag="OQN13_004225"
                     /pseudo
     CDS             complement(38..629)
                     /locus_tag="OQN13_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
BASE COUNT          197 a          159 c          125 g          148 t
ORIGIN      
        1 cagagatgcg tgaattttcc attttctcta ttttccttca gtaacagcag taacgaataa
       61 aataaaaaaa taccggagcg gtgaaaatta atgagtcaat cctgtcaagg attccgccat
      121 gtccaggtaa tagtttcccg ctatctttaa caccaacatc tcgtttgatg gctgacatca
      181 ctacatcgcc gcaaaaacca ctaataccaa ttaacaaacc cgctaatatt gcctgtaatg
      241 tatttagcgg tgtcagtaac ggtccaatga tcatcgacgc tatcatggtg gtgattacgc
      301 cgccgaccag accttccagc gtttttcccg ggctgacttt ggggaccact tttcttctgc
      361 cgcaggattt cccccataaa tactgtgcaa tatcgtttga cgcggttaat gccaaaagaa
      421 ataacaccag taaggccccg ccctggatat tcatggatgg caacattaat aactaggcgg
      481 catgactcaa ggcgaaaacg gtcattatcc agccccaaaa aatggcgctc acggtatgca
      541 gaaaaccgga ggggtcaccc acaaaaactc gccaggcggc taatatcaga aacccagcca
      601 aaggtataaa taacagaaac agctcaaag
//
LOCUS       DAJVRF010000381          622 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJVRF010000381 DAJVRF010000000
VERSION     DAJVRF010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.386"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            353..599
                     /locus_tag="OQN13_004226"
                     /pseudo
     CDS             353..599
                     /locus_tag="OQN13_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
BASE COUNT          180 a          139 c          117 g          186 t
ORIGIN      
        1 gagagacgtt caatggcgga gctcagagaa gactgagatt tgttcaggtt attctgggtc
       61 aacagcgaca ggctgtttgt attaatgact tgtgccatga tcttttcctt atcaattaca
      121 acttgatgtt attgggctgt tgcccacggt ttctcaccgt accccttgta tcggcacctg
      181 aatttcgaac tttagaaaat tttagtttct ctcctgtttt ttcttaacgg ctgaaatatc
      241 cgtaatatac aactctactc tgcgcatcat cttcgtgaat caactattaa actttgcgca
      301 caaattgccg ataacccgct tatctactgt tttcaatcaa aaggaattag gtatggcttc
      361 atttacatct cttggcgttg gttcgaatct gccgctggat acattgctaa acaacctgac
      421 catcgctgaa aaaaaacgct taaacccaat tactcagcaa cagagtgaca ataccgcacg
      481 tctgacagcc tatggcacat taaaaatgtg cattagaaaa attccagact gcaaatactg
      541 cgttaaataa agccgatctg ttcaaaagca ctaccgttac cagtagcaat gaagaccttt
      601 gaggtagcct gagtttaacg ga
//
LOCUS       DAJVRF010000382          610 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJVRF010000382 DAJVRF010000000
VERSION     DAJVRF010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.387"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..261)
                     /locus_tag="OQN13_004227"
     CDS             complement(<1..261)
                     /locus_tag="OQN13_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine (deoxy)nucleoside triphosphate
                     diphosphatase"
                     /protein_id="HCS1617397.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVP"
     gene            348..>610
                     /locus_tag="OQN13_004228"
     CDS             348..>610
                     /locus_tag="OQN13_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCS1617398.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
BASE COUNT          128 a          160 c          158 g          164 t
ORIGIN      
        1 gggtacgtgc caggcatgaa gatggataat ccggcccgaa acttctcgct gatggctggc
       61 aacatactca cccacagttg cttcgatgcc caattcttcg tttaactcac gcaccagtgc
      121 ctgctgctgg ctttcatcca gctcgacttt accatcggca aactcccata atcccgcctg
      181 atcgctctgg gcggggcgtt gcgcgagtaa aattttgcca tcacgttcaa tgatggcggc
      241 aacaacttca atcattttca taaattacac acgggcagat aagcgaaaaa tgccataata
      301 tccgcactgt tgctcaatca gaaaggcttt atatttaagg agtgcctgtg ctagaccgcc
      361 atcttcatcc ccggattaaa ccgttgttgc atcagtgcgt gcgggttctt gataaaccgg
      421 gcattacgcc agatggttta acattagtgg gatttgccat cggcgtgctg gctctgccgt
      481 ttctggcgct gggctggtat ctggcggcgc tagtcgtcat tttgttgaac aggctgcttg
      541 atggtctgga cggtgcgctg gcgcggcgca gagagcttac cgacgcgggc ggctttcttg
      601 atatttctct
//
LOCUS       DAJVRF010000383          594 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJVRF010000383 DAJVRF010000000
VERSION     DAJVRF010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.388"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            59..594
                     /locus_tag="OQN13_004229"
                     /pseudo
     CDS             59..594
                     /locus_tag="OQN13_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
BASE COUNT          140 a          153 c          170 g          131 t
ORIGIN      
        1 ttgccgttgg gtgtagtgta agcgactgaa aaacaaacgc ccctgaaatc atgtccagat
       61 ccggcagaac cagcctcatg cgcagttctg gctggttttt gacgttgatc gcgagggggc
      121 agcgattgac tggagcgacc ggaacgctcc cgcgccgaac atcaccgtaa aaaatcctgt
      181 gaacggtcat gctcacctgc tctacgcgct caatatcgcc gtgagaaccg cgccggacag
      241 ctcggttaag gcgctgaaat acgccgctgc gattgaacgt tcgctgtgtg agaaactttg
      301 tgctgatgtg aactacagcg gactgatttg taaaaatccg ttccacctgg aatggcaggt
      361 gatggagtgg cgagaggaag cctataccct cgatgaactg gctgattatc tcgatttgag
      421 cgcctcagcg cgccgtagca tcgataaaca ttacgggatg gggcgaaact gccacctgtt
      481 cgaaatgacg cgcaaatggg cttacagggc gattcgtcag ggctggcccg aattctcaca
      541 gtggcttgat gccgtgattc agcgtgtcga aatgtacaac gcatcgcttc ccgt
//
LOCUS       DAJVRF010000384          586 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJVRF010000384 DAJVRF010000000
VERSION     DAJVRF010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.389"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>586
                     /gene="gspA"
                     /locus_tag="OQN13_004230"
     CDS             <1..>586
                     /gene="gspA"
                     /locus_tag="OQN13_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HCS1617399.1"
                     /translation="GRPDRKVERDFKKQDIELVSTGRLTEHELKASILEGQNIDQPDL
                     LLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFTAKQWRMIYRILGDNRPR
                     KMQLAVVMSGTIIAITCGWLLLSSFTAPFPVPAWLVPVTPVVKQDMTKDIAHVVMRDS
                     EALSVLYGVWGYEVPADSAWCDQAVRAGLACKSGN"
BASE COUNT          151 a          136 c          160 g          139 t
ORIGIN      
        1 cggcagaccc gatcgaaaag tcgaacgtga ttttaaaaaa caggacattg aacttgtctc
       61 gacaggacgt ctgacggagc acgaacttaa ggcaagcatc cttgaaggtc agaatattga
      121 ccaacccgat ctccttttaa cctccagagt tctgaaacgg atagctttat tatgtcgggg
      181 cgatcgcaga aagctggcgc ttgccggcga aacaattcgc ttattacagc aagccgagca
      241 gaccagcgtt ttcactgcaa aacaatggcg aatgatttac cgcatattgg gtgataaccg
      301 cccccggaaa atgcagcttg ccgtggtaat gagtggaacc attatcgcta ttacctgtgg
      361 ttggctactg ctgtcctctt tcacagctcc gttccccgtt cctgcgtggc tggtacccgt
      421 aacgccagta gttaaacaag atatgactaa ggatattgct catgtggtga tgcgcgatag
      481 tgaggcgcta agcgtgttat acggcgtatg ggggtacgag gtcccagctg attcggcctg
      541 gtgcgatcag gcagtaagag cggggctggc atgtaaaagt gggaat
//
LOCUS       DAJVRF010000385          575 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJVRF010000385 DAJVRF010000000
VERSION     DAJVRF010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.390"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            1..575
                     /locus_tag="OQN13_004231"
                     /pseudo
     CDS             1..575
                     /locus_tag="OQN13_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS629 family transposase"
     misc_feature    246..362
                     /locus_tag="OQN13_004231"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          139 a          138 c          177 g          121 t
ORIGIN      
        1 caacgggcag tccgtatggt tctggaaagt cagggcgaat atgactcaca atgggcgaca
       61 atttgttcca ttgctccaaa gattggctgt acgccggaga ctctgcgtgt ccgggttcgc
      121 cagcatgagc gggataccgg gggcggtgat ggagggctca ccaccgctga acgtcagcgt
      181 ctgaaagagc tggagcgtga aaatcgtgaa ctgcgccgca gtaacgatat ccttcgccag
      241 gcttccgctt attttgcgaa ggcggagttt gaccgcctct ggaaaaaatg atgccactgc
      301 tggataagct gcgtgagcag tacggggtcg gaccgctatg cagcgaactg catattgccc
      361 cgtcaacgta ttaccactgt cagcaacagc gacatcatcc ggataaacgc agtgcccgtg
      421 cgcagcgcga tgactggctg aagaaagaga tacagcgcgt atacgatgaa aatcacaagg
      481 tatacggtgt gcgtaaagtc tggcgtcagt tgttacggga aggtatcaga gtggccagat
      541 gcactgtggc acgtctcatg gcggttatgg gactt
//
LOCUS       DAJVRF010000386          573 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJVRF010000386 DAJVRF010000000
VERSION     DAJVRF010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.391"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>573
                     /locus_tag="OQN13_004232"
     CDS             <1..>573
                     /locus_tag="OQN13_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCS1617400.1"
                     /translation="QILNILENFDMKKYGFGSADAMQIMAEAEKYAYADRSEYLGDPD
                     FVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTTHYSVVDKDGNA
                     VAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGDANAVGPNKRPL
                     SSMSPTIVVKDGKTWLVTGSPGGSRIITTVL"
BASE COUNT          151 a          144 c          152 g          126 t
ORIGIN      
        1 acaaatcctc aatattctgg aaaacttcga tatgaagaaa tacggctttg gcagcgccga
       61 tgcgatgcaa atcatggcag aagcggagaa atacgcctac gccgaccgct cggaatatct
      121 tggcgacccg gattttgtca aagtcccgtg gcaggcactg accaataaag cctatgccaa
      181 atccattgcc gatcaaattg atatcaacaa agcgaagccg tccagtgaga ttcgccctgg
      241 caagcttgcg ccttatgaga gtaatcaaac tacccattac tctgtagtgg ataaagacgg
      301 taacgcggtg gcggtgacct atacgctgaa caccaccttc ggtacgggca ttgtcgcggg
      361 cgagagcggt attctgctta ataatcagat ggatgatttc tccgccaaac cgggcgtacc
      421 gaacatttac gggctggtgg gcggtgatgc caacgccgtc gggccgaata aacgtccgct
      481 gtcatcgatg tcaccaacca ttgtggtgaa agacggtaaa acttggctgg ttaccggtag
      541 tcctggcggg agtcggatta ttaccaccgt gct
//
LOCUS       DAJVRF010000387          570 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJVRF010000387 DAJVRF010000000
VERSION     DAJVRF010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.392"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..532
                     /gene="istB"
                     /locus_tag="OQN13_004233"
     CDS             <1..532
                     /gene="istB"
                     /locus_tag="OQN13_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HCS1617401.1"
                     /translation="GLAFVERSENVILLGPPGVGKTHLAIALGVKAVDAGHRVLFMPL
                     DRLIATLMKAKQENRLERQLQQLSYARVLILDEIGYLPMNREEASLFFRLLNRRYEKA
                     SIILTSNKGFADWGEMFGDHVLATAILDRLLHHSTTLNIKGESYRLKEKRKAGVLTKN
                     TTPISDDEMVESGQHQ"
BASE COUNT          171 a          123 c          165 g          111 t
ORIGIN      
        1 tggtctggcg ttcgtggagc gcagcgaaaa cgtgatcctg ctgggccctc ctggtgtcgg
       61 aaaaactcat ctggccatag ctcttggcgt gaaagcggtg gatgcgggac atcgggtact
      121 gtttatgcca ctggacagac tgatcgcgac actgatgaaa gcgaaacagg aaaaccggct
      181 ggagcgtcag ctacagcaac tgagttatgc ccgggtgttg atcctggatg aaataggcta
      241 tctgccgatg aacagagagg aagccagtct gttcttccgg ctactgaacc gtcgatatga
      301 aaaagcgagc atcatactga cgtcaaacaa agggttcgca gactggggag aaatgttcgg
      361 agatcacgta ctggcaacag cgatcctgga tcggctgcta catcactcaa ccacgctgaa
      421 tatcaaagga gagagttacc ggttaaaaga gaaacgtaaa gctggagtgc tgaccaaaaa
      481 cacaacgcca atcagtgatg atgaaatggt ggaaagcgga cagcatcagt aacgaaagta
      541 tttagcgggc atgaaaatgg caaataacgg
//
LOCUS       DAJVRF010000388          566 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJVRF010000388 DAJVRF010000000
VERSION     DAJVRF010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.393"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(107..>566)
                     /locus_tag="OQN13_004234"
     CDS             complement(107..>566)
                     /locus_tag="OQN13_004234"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308760.1"
                     /note="UV-induced DNA repair; converts cyclobutane-type
                     pyrimidine dimers created during exposure to UV ratiation
                     to monomers; light dependent; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCS1617402.1"
                     /translation="KTGYPIVDAAMRQLNSTGWMHNRLRMITASFLVKDLLIDWREGE
                     RYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRIFNPTTQGEKFDLEGEFIRQWLPE
                     LRNVPGKSVHEPWKWAQKAGVKLDYPQPIVEHKEARVQTLAAYEAARKGK"
BASE COUNT          118 a          170 c          126 g          152 t
ORIGIN      
        1 caggcctacg tatttcctgc aatttattga atttgcacaa atttgtaggc tggataaggc
       61 gttcacgccg catccggcat ctgggctcga ttgcctgacg cgtctgttat ttccccttcc
      121 gcgccgcctc atacgctgcc aacgtttgta ctctcgcctc tttgtgctcg actatcggtt
      181 gcggataatc cagcttcaca cctgctttct gcgcccactt ccacggctca tgcaccgatt
      241 tccccggtac attgcgtagt tccggtagcc actggcggat aaactcacct tcgagatcaa
      301 atttctcgcc ctgggttgtc gggttgaaaa tacgaaaata cggcgctgca tcggttccgg
      361 ttgaagcggc ccactgccag ccaccgttat tggctgccaa atcaccatca atcagctgcg
      421 acatgaaata tcgctcgcct tcgcgccagt cgatcaataa atctttcacc agaaaactgg
      481 ctgtaatcat ccgtagcctg ttatgcatcc agccagtgct gttaagctga cgcatagcgg
      541 catcaacaat cgggtatccc gttttg
//
LOCUS       DAJVRF010000389          557 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJVRF010000389 DAJVRF010000000
VERSION     DAJVRF010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.394"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>557
                     /locus_tag="OQN13_004235"
     CDS             <1..>557
                     /locus_tag="OQN13_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1617403.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICAGRPDEGIPAVCFKLK"
BASE COUNT          111 a          162 c          159 g          125 t
ORIGIN      
        1 gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgccgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca caaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctcggtggt cagattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtgat tgcacagtac tatgaattcc tgcgccttgg tcgtgaaggc
      421 tataccaaag tacagaatgc ttcctaccag gtcgctgcct atctggcgga tgagatcgcc
      481 aaactggggc cgtatgagtt catctgtgcc ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaaga
//
LOCUS       DAJVRF010000390          553 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJVRF010000390 DAJVRF010000000
VERSION     DAJVRF010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.395"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            1..553
                     /locus_tag="OQN13_004236"
                     /pseudo
     CDS             1..553
                     /locus_tag="OQN13_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT          196 a          121 c           84 g          152 t
ORIGIN      
        1 tatccgactt attaaaaatg cctgtttaaa attatataat ttagcacata ccgcaaccaa
       61 ctcccacctt aaaccaactg gtccggataa ttcagatgtt ctcctgttca aaaagctatt
      121 ctccccttcg aaattaatga caattatcgg tgatgaaatt cctcttatat ctgaaaaaca
      181 gtcgctttca aaggtgcttt taaatgatga gaataatgaa ctgagtgatg gtacaaactt
      241 ctgggataaa aatcgtcaat taaccacaga tgaaatagct tgctatcttc agaagatcgc
      301 cgctaatgca aaaaatactc aagtcaatta tcctactggt ctctacgtcc cctactccac
      361 cagaactcac ctggaagacg ctctcaatga aaatattaag agcgatccat catggccgaa
      421 tgaagtccag ttattcccca taaatactgg cggacactgg atattagttt cgctacagaa
      481 aatagtaaat aaaaaaataa taaactacaa ataaaatgcg tcatattcaa ctcattgcgt
      541 gcactaggcc atg
//
LOCUS       DAJVRF010000391          551 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJVRF010000391 DAJVRF010000000
VERSION     DAJVRF010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.396"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            43..303
                     /locus_tag="OQN13_004237"
     CDS             43..303
                     /locus_tag="OQN13_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617404.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
     gene            300..>551
                     /locus_tag="OQN13_004238"
     CDS             300..>551
                     /locus_tag="OQN13_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617405.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRA"
BASE COUNT          131 a          153 c          166 g          101 t
ORIGIN      
        1 tgatttttac ctgtcagaac attatcagat ggaacgtgca ggatggatca ggaaatgacg
       61 ttttcactca gctatgaaca actgacccgg tttgcggaaa aacgcatccg ggaatgcaat
      121 ctggacagcc acggcgtaac ttatctctgc gagtcagcca aagccggagc cgtgctgatc
      181 ttctggcatg aactggccat aaatggctat acctcaatga atgccataaa gagacaggag
      241 atcattgacg ccgaccatca gcgcctcaga aagctcatct ggccggagga tgactggaaa
      301 tgactcagag ccgccgaccg tcgccactgc agcggagagt gctgattgtg ctggctgccc
      361 ttgatgaaaa acggccagga cccgtgctga cgcgggacat agaacgggtg ctggagcaaa
      421 gcggggaagc cccggtctac gggccaaacc tgcgcgcctc ctgccgtcgt ctggaggatg
      481 ccggctggct gcgtaccctg cgcgctccga acctgcagct ggcagtggag ctgacggacg
      541 ccggccgggc c
//
LOCUS       DAJVRF010000392          550 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJVRF010000392 DAJVRF010000000
VERSION     DAJVRF010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.397"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>550
                     /gene="feaR"
                     /locus_tag="OQN13_004239"
     CDS             <1..>550
                     /gene="feaR"
                     /locus_tag="OQN13_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HCS1617406.1"
                     /translation="QEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDASR
                     PCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNNP
                     ALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWIA
                     GETGMSVRSLYRMFADKGLVVA"
BASE COUNT          139 a          125 c          146 g          140 t
ORIGIN      
        1 ggcaggaagt aaaaggcagc gacgatgcct ggttttacac cgtttttcag cttagtggtc
       61 aggcaataat ggagcaggat gagcgtcagg tgcagattgg cgctggcgat attacgttac
      121 tcgatgcctc acgcccctgt tcgctttact ggcaggagtc ttctaaacag atttcattac
      181 ttttgccacg cactctgctg gaacaatatt ttccccatca aaaacctgtc tgcgcagaaa
      241 gactggacgc tgacttaccc atggtgcaac tcagtcatcg cctgttacag gagagcatga
      301 ataatccggc actttctgaa acagaaagtg aagctgcgct acaggcgatg gtgtgtctgc
      361 tgcgcccggt acttcatcag cgggaatctg ttcaacctcg ccgtgaacgt cagtttcaaa
      421 aagtggttac gttgatagac gataatattc gcgaagagat attacgcccg gagtggatag
      481 ccggagagac aggtatgtca gtacgtagtt tgtaccgaat gtttgccgat aaaggtttgg
      541 tagtcgcgca
//
LOCUS       DAJVRF010000393          550 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJVRF010000393 DAJVRF010000000
VERSION     DAJVRF010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.398"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..177
                     /locus_tag="OQN13_004240"
     CDS             <1..177
                     /locus_tag="OQN13_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HCS1617407.1"
                     /translation="GAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAG
                     QKKATNPFLKIIES"
     gene            174..>550
                     /locus_tag="OQN13_004241"
     CDS             174..>550
                     /locus_tag="OQN13_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCS1617408.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISA"
BASE COUNT          136 a          144 c          162 g          108 t
ORIGIN      
        1 ggtgcaatgg gcggcagggt caaaaatccg gagctgaccg ccaaaaaaga acaggagtcc
       61 gagatgagca gcacgggggc aatgctcgga ctcgacccca gcagccgcca gcgtctgatt
      121 ggcctggcgg ggcagaagaa agccactaac ccgtttctga aaattatcga atcatgagcc
      181 ggaaatctta ccccaacgta aatgctgcaa atcagtatgc ccgggatgtc gtgcgcggaa
      241 agattgttgc ctgccagttt gtgattcagg cctgccagcg ccatcttgat gacctgatgg
      301 cggaaaaaag taagtcgttt cgttaccgct tcgacaagga cctggctgaa cgggccgcga
      361 aatttattca gctgttgccg cacaccaagg gggagtgggc attcaaacgg atgcccatca
      421 cgctggagcc gtggcagcta tttgtgatct gctgtgcgtt tggctgggtc aataaaggca
      481 cccggttgcg ccgcttccgg gaggtgtaca ccgaaatccc ccgtaagaac ggcaaatcag
      541 caatctctgc
//
LOCUS       DAJVRF010000394          533 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJVRF010000394 DAJVRF010000000
VERSION     DAJVRF010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.399"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..120)
                     /locus_tag="OQN13_004242"
     CDS             complement(<1..120)
                     /locus_tag="OQN13_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxamate siderophore iron reductase FhuF"
                     /protein_id="HCS1617409.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHL"
     gene            261..497
                     /locus_tag="OQN13_004243"
     CDS             261..497
                     /locus_tag="OQN13_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCS1617410.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
BASE COUNT          148 a           95 c          145 g          145 t
ORIGIN      
        1 caaatgttca cgatgtttgg cgatcgtcgc acgtacggct tgtgcaagcc cagcatcctg
       61 cggctggaga tgcgttcgcc agatgatatc ttcatagagc ggtgcggaac gataggccat
      121 aatcgggata gtaatctaaa tgataatgat tgctaatcct agcgataggt ttacccgata
      181 gcaagggatt tctctggctt gcaaatgata aaaattatca tatgatattg gttatcatta
      241 tcaatgaaag agatgaaacc atgttgcaac gtacgctggg cagtggctgg ggagtgttgc
      301 tgccgggatt gctgattgca gggctgatgt atgcggattt atcgccggat cagtggcgga
      361 ttgtcattct gatgggatta gtattgacgc cggtgatgct gtatcacaaa cagttacggc
      421 attacgtttt gctaccatcg tgcctggcgc ttattgctgc catcatgctg atgataatga
      481 atttgaatca gggatgaaaa atcaaggaag aaacaagaaa ggaagtaaag ata
//
LOCUS       DAJVRF010000395          524 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJVRF010000395 DAJVRF010000000
VERSION     DAJVRF010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.400"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(261..>524)
                     /locus_tag="OQN13_004244"
     CDS             complement(261..>524)
                     /locus_tag="OQN13_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCS1617411.1"
                     /translation="AEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSG
                     IRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          130 a          128 c           82 g          184 t
ORIGIN      
        1 catggtttat tcctcttcat ctgaattgat ttacattagg ttggttattt tctcataccc
       61 acaactatat tttctctctg aatttattac gtgcaagaca atcgcagcac tctaactaac
      121 ttcgatgcaa tttgatgttc agaaaaatcc ctcattagat tattatcatg aaatacagct
      181 aatcatcttt agcttatata tttctcaatg tattcttttg aaaggatccg tgccctttca
      241 aactcttcag cagggcaaga ttacgaaagc ccgctccccg caaggactga cgccagatag
      301 tttctgtcca tggctgcttt tcgcatctta cgtcttaacc ctgccttgaa taccttatca
      361 ttcgtcaaaa tattaatagc aatgtgccgt atccctgaaa ataattctgc tgcatttcct
      421 cttcttattt tgcagtcgtc ttcattcatt accacgtcca gacgccagtg cagcttattc
      481 tccacgtgcc agtggtttcg gattgctgtg gcgaactttt ctgc
//
LOCUS       DAJVRF010000396          520 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJVRF010000396 DAJVRF010000000
VERSION     DAJVRF010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.401"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>520
                     /locus_tag="OQN13_004245"
     CDS             <1..>520
                     /locus_tag="OQN13_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1617412.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLA"
BASE COUNT          138 a          121 c          117 g          144 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc
//
LOCUS       DAJVRF010000397          507 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJVRF010000397 DAJVRF010000000
VERSION     DAJVRF010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.402"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            224..>507
                     /locus_tag="OQN13_004246"
     CDS             224..>507
                     /locus_tag="OQN13_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1617413.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSL"
BASE COUNT          173 a           96 c          109 g          129 t
ORIGIN      
        1 gcttaaagat tttaacgatc gcttattgta aaaataatag caaagaaaat tgtaagatac
       61 agaccgtaaa taaaaacatc ttccttggtc aggaaagaaa tcttgaaagc atatctggct
      121 acgacaaaat cgttgtcaaa tataataact ggatcaccaa agataatggc gagtttgaac
      181 tggcggtcca ttaattatca ttgtaataaa cgaaggaata gtaatgaaaa aggtatttgc
      241 aaaatctctt ctggtcgcag cgatgttttc tgttgcaggc tccgcattgg cagtacaaaa
      301 ggatattact gtaacggcca acgttgatgc cgctctggat atgacacaga ccgataacac
      361 cgcgttgccg aaagcagtag aaatgcaata tctgccgggg cagggtctgc aatcttacca
      421 gttgatgacc aaaatctggt ctaacgacgt taccaaagat gtaaaaatgc agttagtttc
      481 cccggcgcaa ctggttcaaa gcctgga
//
LOCUS       DAJVRF010000398          506 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJVRF010000398 DAJVRF010000000
VERSION     DAJVRF010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.403"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(1..468)
                     /locus_tag="OQN13_004247"
                     /pseudo
     CDS             complement(1..468)
                     /locus_tag="OQN13_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT          168 a          112 c          109 g          117 t
ORIGIN      
        1 aaatggcagc aatggtttga ttaaatgttg aatcttcaca cccgagatag aacaaccaac
       61 aaatagcgca ctacccaccg gcggtgttaa cagcccaata caaaggttgg ccaccatcat
      121 aataccgaaa tgtactgggt ccattcccag ctcttgtgca atcggcaaga agatcggtgt
      181 aaagattagt acagccggtg tcatgtccat aaagatgccg acaattaata agacgatatt
      241 aatcagcagc aatatgatca gtgggttatc agaaatcccc atcaatgcgt cgctgatcat
      301 ataaggaata tcagcattag tcattgccca ggacatgccc acggaaaaac cgatcaacag
      361 taatacaatc gaagtggtaa caaccgactc cagaatcagt ttgggtaaat ggcgccattt
      421 tacttcccgg taaatcagca ccgagagaat aaatgtgtag acaacggcgg tgatgctgcc
      481 aacttactga tttagtgtat gatggt
//
LOCUS       DAJVRF010000399          506 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJVRF010000399 DAJVRF010000000
VERSION     DAJVRF010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.404"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..188)
                     /locus_tag="OQN13_004248"
     CDS             complement(<1..188)
                     /locus_tag="OQN13_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1617414.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVRVRQHERDTGGGDG"
     gene            242..445
                     /locus_tag="OQN13_004249"
                     /pseudo
     CDS             242..445
                     /locus_tag="OQN13_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          126 a          135 c          117 g          128 t
ORIGIN      
        1 ccatcaccgc ccccggtatc ccgctcatgc tggcgaaccc ggacacgcag agtctccggc
       61 gtacagccaa tctttggggc aatggaacaa attgccgccc actgtgagtc atattcatcc
      121 tgactttcca gaaccatacg aatcgcccgc tgacggactt cgggggaaaa acgagtattt
      181 ttagtcatcc tgtttacctc tttctcaggg agtttagtct ccaggattcc cggggcggtt
      241 cagacatcta gaatttactg tcagcaccgg cgttaaagtt tacttctgcg atcctcagag
      301 tccttggcag cggggaacaa atgagaatac aaatgggcta attcggcagt actttcctaa
      361 aaagacatgt cttgcccaat atactcaaca tcgagtaggc agcctgacgg ctgcggcttg
      421 tcatggtctg caattaccgt tataaaaaca ggcaatgtca ttgtctttca ggtggttata
      481 tggcccgttc agctaaaccc cgtaaa
//
LOCUS       DAJVRF010000400          499 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJVRF010000400 DAJVRF010000000
VERSION     DAJVRF010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.405"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..>499)
                     /gene="espL1"
                     /locus_tag="OQN13_004250"
     CDS             complement(<1..>499)
                     /gene="espL1"
                     /locus_tag="OQN13_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HCS1617415.1"
                     /translation="RNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALLRHPPAGSRV
                     IASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSVYGQLNYVVSVYD
                     PNDTNVAVRGTHRAARGFLSLDKFISSAPDAQTWADRYVRNCAIAILPLLPEGVPVAI
                     LAGITT"
BASE COUNT          144 a          114 c          115 g          126 t
ORIGIN      
        1 gtcgtgatac ccgctaaaat agcaactgga actccctcag gtaatagagg aagaatagca
       61 attgcacagt tgcgaacata cctatcagcc caggtctgag catcgggagc tgaactgatg
      121 aacttatcaa gcgagagaaa gccccgtgct gccctgtggg tgcctctgac ggcaacgttg
      181 gtatcgttcg ggtcgtaaac agacacaaca taatttaatt gtccatacac tgactgcttt
      241 attcgtagcc ttactgacaa agtatgattg tctatatata aaatcgcgga catatgactg
      301 acattgtttg ccatcattcg acaaaagaaa atatctaaag cgaagccaaa atccttactc
      361 gcgataacgc gactccctgc aggaggatgg cgaagtaatg ctctgaactc cgcgtcccgg
      421 actaagggga catgctgctg tatggattca acggaggagt agtgtttaat caggttgact
      481 ttgccttttc catttctca
//
LOCUS       DAJVRF010000401          496 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJVRF010000401 DAJVRF010000000
VERSION     DAJVRF010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.406"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..180)
                     /locus_tag="OQN13_004251"
     CDS             complement(<1..180)
                     /locus_tag="OQN13_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HCS1617416.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECW"
BASE COUNT          136 a          101 c          124 g          135 t
ORIGIN      
        1 ccagcattcg ccctcttcgg tcaccagcac cggcactttt cctaacgggt taaattgcgc
       61 cacgccgttg tccgcgttat agggcagttc attaataaat tcgaaagtta tgcccttttc
      121 taacaacaga atagaaagtt tgcgtacaaa cgggctggtg tagctaccga cgagtttcat
      181 gccgagtcct ttgtgcgagg aaaaatatca gtatggcctg gtgatgggaa aaagggcaga
      241 aaatgttgat ggtttgttac ttccaaataa atcacatatt tatcatggtg atataaatat
      301 tttcctaatt gtttctatct gatggatatc tcacttaagg ctttcttata aatctgtagg
      361 gttttgcctg gaagcagaca aataacccga taaaacaagg atgagcagat gagcggaaaa
      421 ccggcagcgc gtcagggcga catgacgcag tatggcggta gcattgttca gggttcagcc
      481 ggggtgcgca tcggtg
//
LOCUS       DAJVRF010000402          495 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJVRF010000402 DAJVRF010000000
VERSION     DAJVRF010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.407"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..264)
                     /locus_tag="OQN13_004252"
     CDS             complement(<1..264)
                     /locus_tag="OQN13_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCS1617417.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWS"
BASE COUNT          167 a          111 c          100 g          117 t
ORIGIN      
        1 actccaatta attggatgat caccacctct gacgccaatc agggaggtgg tgagacgtac
       61 agggttggcg taaccggtca tcacaaaacc cggcgcggat ttctccgccc ctgcacgccc
      121 caccataatg cgaatgtggc aatgcttaac gcaaaaaaaa ccgcaaacgc ggctatgcgt
      181 tgtgatgtta tccgagacgc caatcccgac aaccgatttt gcggttgtgt gaggaatata
      241 gccccggaga acaaaaactg tcaacgggca aaaaaatttc cgttatcagg aatgtataaa
      301 attaaagcga tcgagcaaaa aatcctttct gcaaaactaa aaaagatttc aataaaatca
      361 ggcagttaaa aacacggaaa acggtataaa aaagcgcctc aaaatcctgt tttttctttc
      421 agatacaatg tcttattgca ttttttaaaa catgtcagtg tggtacatgg atatcgatac
      481 catcgccaat tataa
//
LOCUS       DAJVRF010000403          491 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJVRF010000403 DAJVRF010000000
VERSION     DAJVRF010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.408"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(13..>491)
                     /locus_tag="OQN13_004253"
     CDS             complement(13..>491)
                     /locus_tag="OQN13_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617418.1"
                     /translation="VGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGKYDDDGFFVPL
                     ADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVNLGSDFDASTI
                     KKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADGNAEISWKI"
BASE COUNT          112 a          141 c          125 g          113 t
ORIGIN      
        1 ttcgtctgtt ccttaaatct tccaggaaat ttctgcattg ccgtcagcat caccggcacc
       61 tgtgaattca gcgttggtca gcaccacgtt tttgccactg actgacgtgg acatgaatcc
      121 acccagcggc actttgatgg attcatcagt ggagacgaca acgtataccg gatcgccttt
      181 tttgatagtg ctggcatcaa aatcagaacc gagattaacg gtcacgtagc cacgcttcat
      241 ggcgtcgccc gggaagttct tgcctgttcc cacctggcga accatgtccg gctgcgacgt
      301 ggtcggataa gggcgcacgt agatcccctt caccttgtct gcggtatcac catctgccag
      361 cggcacgaaa aaaccgtcat catcgtattt accagccagg ccataggcag cgaaggcgtt
      421 atcggattta aggaccaccg gttcgacggt taagtcctgc gggcgagaga cagccccggc
      481 aatgccaaca g
//
LOCUS       DAJVRF010000404          481 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJVRF010000404 DAJVRF010000000
VERSION     DAJVRF010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.409"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
BASE COUNT          190 a           76 c           65 g          150 t
ORIGIN      
        1 gtatagaggc ggttatgtaa aaccactcgt tagcctcaaa acaaagacaa atttccagat
       61 ttattactta atcaatgaat aacaagctta aaaaacaaac tattaaccta aagaaaaaat
      121 taacagacat taattcaata ttaaaaaaat aatagattaa aatttcttaa cgatttaaga
      181 atcatacaaa taacactttg attaaattta attttttgta tcgataattg tgaacctctc
      241 tggcatggag aactattttg aacatgaggt gttacgtgga tattttgctt attacaagta
      301 ctgctaatat aaaaacttga gaaagagata acgggttata tggtggttta tccccgctgg
      361 cgcggggaac actctaaaca taacctatta ttaattaatg attttttaag ccagtcacaa
      421 tctaccaact ttacagtatc acacaaacaa cacatccatt atgttaaaga gtacttaatt
      481 c
//
LOCUS       DAJVRF010000405          479 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJVRF010000405 DAJVRF010000000
VERSION     DAJVRF010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.410"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..113
                     /locus_tag="OQN13_004254"
     CDS             <1..113
                     /locus_tag="OQN13_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="The transcriptional repressor of DicA (divison
                     cell) has a hypothetical DNA binding domain. It represses
                     the transcription of dicB and dicC, but its specific role
                     in cellular replication and mode of action are still
                     unknown; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1617419.1"
                     /translation="PESEQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            317..472
                     /locus_tag="OQN13_004255"
     CDS             317..472
                     /locus_tag="OQN13_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCS1617420.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
BASE COUNT          154 a          116 c           92 g          117 t
ORIGIN      
        1 tgcctgagtc agagcaaaag gccctgttgt cagagatgcg tgctcgagtt gaaaatttca
       61 acaaactttt tgaagaacta ctcaaagctc gcaaaagaag cgcaaacaaa taatcccctt
      121 ttttctccac accctgtaat aaaaagcaca aactttcaaa cacttgtgtt ttttacatca
      181 aaaaaactta ggtttttcta cacaaaaatc ttgaccgccc accttaggtt attctaaatt
      241 tcattcatca agacaccgca cggtgttctc agcaaacagt tccgctaccc cggcgttaag
      301 gggaaatggg gtcaacatga atactatcga tcttggcaac aacgaatctc tggtgtacgg
      361 cgtgttcccc aaccaggacg gcacattcac cgccatgacg tataccaaaa gcaaaacgtt
      421 taaaaccgaa gctggcgcac gtcgttggtt gggaagacat tcaggtgagt aaaatgaac
//
LOCUS       DAJVRF010000406          476 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJVRF010000406 DAJVRF010000000
VERSION     DAJVRF010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.411"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>476
                     /locus_tag="OQN13_004256"
     rRNA            <1..>476
                     /locus_tag="OQN13_004256"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAJVRF010000407          475 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJVRF010000407 DAJVRF010000000
VERSION     DAJVRF010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.412"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..>475)
                     /locus_tag="OQN13_004257"
     CDS             complement(<1..>475)
                     /locus_tag="OQN13_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCS1617421.1"
                     /translation="TRTEVSTFCISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFDQEG
                     QYQDYQRTQYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIV
                     LTGHPQANLNREWQVVASELHGEQPQAVPGRSGSGTTLNNHFAVIPADRTWRPQPL"
BASE COUNT           80 a          150 c          134 g          111 t
ORIGIN      
        1 caacggctgt ggccgccatg tccggtctgc cggtattacc gcaaagtggt tattcagcgt
       61 ggttcctgaa ccactgcgtc ccggcaccgc ctgcggctgc tcgccgtgca gttcacttgc
      121 caccacctgc cattcccggt tcaggttcgc ctgcggatgc cccgtcagca caattcgccg
      181 tcccggccat atctccggag aacggcttgt tccgcgcgcc acttctgcgt tgttgcgcca
      241 gccatccatc tgccagcggg caaagttctg cccgtgagca cccttgaaac gtccggggta
      301 gtcatacact tcatactgtg tgcgctggta atcctggtac tggccttcct gatcaaaacg
      361 ccctgcccag ccgggtcgtt taaaggtgta gtctttggtc accacggaag aaggacggat
      421 ttgtgcgctg tagcggaact ggctgatgca gaaggtactt acctcggtac gggtg
//
LOCUS       DAJVRF010000408          474 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJVRF010000408 DAJVRF010000000
VERSION     DAJVRF010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.413"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(260..>474)
                     /locus_tag="OQN13_004258"
     CDS             complement(260..>474)
                     /locus_tag="OQN13_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCS1617422.1"
                     /translation="ANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIR
                     VSRLAEPFWQDHFLGARRILTEETIL"
BASE COUNT          141 a          119 c          103 g          111 t
ORIGIN      
        1 ttaattatgt aaggagtatt tgatcatgca taagcattaa aaaacagttt caagtaatga
       61 ggaatatctc aaattttaaa cataaaatgc gaattattta gtacaaaaag tgtacttctg
      121 atgatctttc cctgggtgat ggaataacag gaaagcggct cgcaaaaacg cacagatttg
      181 taggcatgat aagacgcgtc agcgtcgcat caggcatctg cgcaccaccg ccggatgcgg
      241 cgtaaacgcc ttatccgtcc tacaaaatcg tctcttccgt caatatccgc cgcgcgccca
      301 aaaaatgatc ctgccagaaa ggttcagcta accgacttac ccgaatggtt tccccggtgc
      361 gcggtgactc aataaactga ccgtcgccca gatacacccc catatggtcg gcaatctcgc
      421 ggctgtggat atggaaaaac agcaaatctc cccggcgcag gtcgttattc gcca
//
LOCUS       DAJVRF010000409          473 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJVRF010000409 DAJVRF010000000
VERSION     DAJVRF010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.414"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..466
                     /locus_tag="OQN13_004259"
     CDS             <1..466
                     /locus_tag="OQN13_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Hsp31 stabilizes early unfolding protein
                     intermediates under severe heat stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCS1617423.1"
                     /translation="PKKLADVVASLNADSEYAAIFVPGGHGALIGLPESQDVAAALQW
                     AIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTPEIGYMPGHLTWYF
                     GEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQEMLAAYAD"
BASE COUNT          115 a          122 c          124 g          112 t
ORIGIN      
        1 tccgaagaaa ctcgcggatg ttgtcgccag cctcaacgct gatagcgaat atgcagcaat
       61 ctttgttcct ggtggtcatg gcgcacttat tggtttacca gaaagccagg acgtagctgc
      121 tgctttgcag tgggcaatca aaaatgaccg ttttgttatc tccctttgcc acggcccggc
      181 ggcttttctg gcgcttcgcc acggcgataa cccactgaat ggttattcca tttgcgcatt
      241 cccagacgcc gcagacaaac aaacgccaga gataggctat atgccgggtc atctcacctg
      301 gtacttcggc gaagagctga agaaaatggg catgaacatt attaatgacg acatcaccgg
      361 gcgagtacat aaggaccgta aagttctcac cggcgacagt cctttcgcag cgaatgcgtt
      421 gggtaaactg gcggcgcagg aaatgctggc agcttatgcg gattaatccc tgt
//
LOCUS       DAJVRF010000410          472 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJVRF010000410 DAJVRF010000000
VERSION     DAJVRF010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.415"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..459)
                     /locus_tag="OQN13_004260"
     CDS             complement(<1..459)
                     /locus_tag="OQN13_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001657922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1617424.1"
                     /translation="MKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEEDLHIQLFE
                     RTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPTAVFYFLP
                     LAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT"
BASE COUNT          135 a           99 c           83 g          155 t
ORIGIN      
        1 ggtaaaatca atggatgaat ttgtgacgtt gttcatgttt atgccaaagt ctgattcgtt
       61 acacagcacc gattccatgc aattattcgt gccttgttcc agaatccgca ctttaatatt
      121 gggatacagc tcattaaatt taccgattgc cagcggtaaa aaataaaata ctgcggtcgg
      181 aatacacgct aacgtcacca taccacgatg ataagcattc atatcacgaa tattaaaaag
      241 cgtttcatcg aatttcttta ttaattcccg cgcctccggg agcaaccttt ttcctgcttt
      301 tgttaacgta accttgcgag ttgtacgctc aaatagttga atatgtaaat cttcctccat
      361 cttttttatt cttcgcgtta atgcaggttg cgtaatattg agtaatttag cggcattgtt
      421 aaacgaactg gactctgcca gtatgacaaa tgccttcata cttgataatt ca
//
LOCUS       DAJVRF010000411          467 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJVRF010000411 DAJVRF010000000
VERSION     DAJVRF010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.416"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>467
                     /locus_tag="OQN13_004261"
     CDS             <1..>467
                     /locus_tag="OQN13_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCS1617425.1"
                     /translation="VPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDFALSMIK
                     LRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAATIDSIS
                     GGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKSD"
BASE COUNT           96 a          134 c          124 g          113 t
ORIGIN      
        1 tcgtacctat tggcaacaac ggctggctca tttcgaccca cgcgccgcag tacatgccga
       61 cctttgaact gaataaagcc attgtgcaga aagcggagca ttaccatttc gatttcgccc
      121 tgtcgatgat caaactgcgt ggctttggcg gcaaaactga gttctgggat cacaaccttg
      181 agtcgttcac cttgatggcg ggtctggcag ccgtgacctc gcgcattcag atttacgcca
      241 ctgctgccac cttaacgtta cctccggcaa tcgtcgcccg tatggccgca accatcgact
      301 ccatctctgg cgggcgtttt ggcgtcaacc tcgtgactgg ctggcaaaag cccgtgtatg
      361 agcagatggg tatctggcct ggcgatgact atttctcccg tcgttacgac tatctcaccg
      421 aatatgttca ggtgctgcgc gacctgtggg gcacggggaa aagcgat
//
LOCUS       DAJVRF010000412          466 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJVRF010000412 DAJVRF010000000
VERSION     DAJVRF010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.417"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..68)
                     /locus_tag="OQN13_004262"
     CDS             complement(<1..68)
                     /locus_tag="OQN13_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCS1617426.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASV"
     gene            complement(68..283)
                     /gene="essD"
                     /locus_tag="OQN13_004263"
     CDS             complement(68..283)
                     /gene="essD"
                     /locus_tag="OQN13_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HCS1617427.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          138 a          120 c          102 g          106 t
ORIGIN      
        1 acagatgcta tagcaatagc cccgccacca atagcagcag caacagcttt tcgtaatgat
       61 ggaggcatta tccacctctc gcagccttgc gcttatcttc tttaatcttg aaataaaggt
      121 ttgtcaggta cgtcagcagg ccaaatacca ggctacccag cactccaatt gccgcccact
      181 gtgagggcgt gactttatct agcagctgta aaaaccagta cccggcacta cctgctgagg
      241 tgccataggc gacacccgtt gttaacttat ccatggattt cataacccca cctcgcagac
      301 aaagcgggtg taaattgagg gaatactacg aaacgtaaca gactcggagt cagtgaataa
      361 ctcaggtatt gggttatcag ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg
      421 gcgggtttaa gctgtgtgac gaagtaacca ctcttaacag cataac
//
LOCUS       DAJVRF010000413          463 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJVRF010000413 DAJVRF010000000
VERSION     DAJVRF010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.418"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>463
                     /locus_tag="OQN13_004264"
     CDS             <1..>463
                     /locus_tag="OQN13_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1617428.1"
                     /translation="RYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRY
                     DHDRFGQMTAVHREEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTTVIAPDG
                     SRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLD"
BASE COUNT          115 a          134 c          135 g           79 t
ORIGIN      
        1 cgttatgata atccacacag tgacttaccc tgcgcaacgg aagatgccac cggcagccgg
       61 aaaaccatga cgtggagccg ttacggtcag ttgctgagct tcaccgactg ttccggttat
      121 gtaacccgtt atgaccatga ccgcttcggg cagatgacgg cggtgcaccg cgaggaaggg
      181 ctgagtcagt accgcgcata cgacagccgt ggacagttaa ttgccgtgaa agacacgcag
      241 ggccatgaaa cgcggtatga atacaacgcc gccggtgacc tgaccaccgt cattgccccg
      301 gacggcagca gaaacgggac acagtacgat gcgtggggaa aagctatctg taccacgcag
      361 ggcggtctga cgcgcagtat ggaatacgat gctgccggac gggtcatccg cctgaccagt
      421 gaaaacggca gccacaccac cttccgttac gatgtactcg acc
//
LOCUS       DAJVRF010000414          459 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJVRF010000414 DAJVRF010000000
VERSION     DAJVRF010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.419"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="OQN13_004265"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="OQN13_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCS1617429.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
BASE COUNT          127 a          127 c           98 g          107 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcaca
//
LOCUS       DAJVRF010000415          458 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJVRF010000415 DAJVRF010000000
VERSION     DAJVRF010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.420"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..215
                     /locus_tag="OQN13_004266"
     CDS             <1..215
                     /locus_tag="OQN13_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1617430.1"
                     /translation="NAMAESINGLYKAEVIHRKSWKNRAEVELAILTWVDWYNNRRLL
                     ERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            257..458
                     /locus_tag="OQN13_004267"
                     /pseudo
     CDS             257..458
                     /locus_tag="OQN13_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          149 a          108 c          107 g           94 t
ORIGIN      
        1 acaacgcgat ggcggagagc atcaatggtc tttacaaagc ggaggtaata caccgtaaga
       61 gctggaaaaa ccgtgcagaa gtggaactgg ccatactcac gtgggtggac tggtataaca
      121 atcgacgatt gctggaaagg ctgggccata ctcctccggc agaagcagaa aaagcttatt
      181 atgcttccat cggaaacgat gatctggcag cctgagttca cagataaaac actctccagg
      241 aaacccgggg cggttcaacc ttcacgataa aaaacttttc attaaaggta aagcggatca
      301 atatccggca cttcattctc tgatatctcc gacccgcatt aatctgaaag aaatagaaat
      361 tcactggcgt gaagtgctac gtctggcgac ctcaataaaa cagggaacag tgacagcatc
      421 cctgatgctg agaaaacttg ccagttaccc caaacaaa
//
LOCUS       DAJVRF010000416          450 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJVRF010000416 DAJVRF010000000
VERSION     DAJVRF010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.421"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            156..260
                     /locus_tag="OQN13_004268"
                     /pseudo
     CDS             156..260
                     /locus_tag="OQN13_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          148 a           87 c           88 g          127 t
ORIGIN      
        1 tttcaaccag taaaaaaacg taaacaccat tcgcatgaat ttcgtagtta agatctgaag
       61 cttgcagaat gcatcggtgt tactgccgga gtccgtgaac ttagtctgca tgaatcacca
      121 cagctttaca tctgtcgcag taaacagcaa aatcagcaaa cttacttaat tatattgagt
      181 gcgattacaa tcgctggcga tggtacagtt ggtgtggcgg tctcagtccg gaacaatttg
      241 aaaatcagaa tctcacttaa agcggcatcc attttacaaa agatggccgt ctgtgtattt
      301 gtaatgaata ccttctacat gtttagagtg cataatccac gacaaggcga tatcctaaaa
      361 attagctagc aggcaacttt gatggcatag ataatgtttt tagtaaaaaa gagttgactg
      421 aatataatac agcctcttcc tacataagaa
//
LOCUS       DAJVRF010000417          445 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJVRF010000417 DAJVRF010000000
VERSION     DAJVRF010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.422"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..344)
                     /locus_tag="OQN13_004269"
     CDS             complement(<1..344)
                     /locus_tag="OQN13_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617431.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKL"
BASE COUNT          145 a          100 c           61 g          139 t
ORIGIN      
        1 tgtaacttct cgatactgag cggctcaata cctttagcct gataataatg cagttgttct
       61 ttttttgctt taaaaccggc ccgaacaata agccccatca taattaatag ataaagaaaa
      121 gagcatcccg cggtaatcag gcctctttca ttcaaaccgt tggatgttat cgctgcgaac
      181 acaaacatta cagcgacaac acatgttaaa taaaaccccc acttacaaag cagcatggcc
      241 ttattttctt taatcatccg ttcaaaatta ctattaaata tttcccagcc attaaaagaa
      301 tacttctcgc tcccaggatg gttttgtaat aaaacttttt tcatcacatt ccctgttatt
      361 acatacttta gaggcgataa ttttatccat gcaaaaaaaa tatccaacga attttcttga
      421 tccgctggat atttcagaat atgac
//
LOCUS       DAJVRF010000418          444 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJVRF010000418 DAJVRF010000000
VERSION     DAJVRF010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.423"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>444
                     /locus_tag="OQN13_004270"
     rRNA            <1..>444
                     /locus_tag="OQN13_004270"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          123 a           90 c          139 g           92 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggca
//
LOCUS       DAJVRF010000419          442 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJVRF010000419 DAJVRF010000000
VERSION     DAJVRF010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.424"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(176..>442)
                     /locus_tag="OQN13_004271"
     CDS             complement(176..>442)
                     /locus_tag="OQN13_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCS1617432.1"
                     /translation="SPKLNMRGGYDVLSQALERANEIKHPVGRVRDIEALDELLATLT
                     DDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHKYLNIA"
BASE COUNT          121 a           99 c           96 g          126 t
ORIGIN      
        1 actttaccgc aataattttc actccagcga aaaattaatg ccacagaatt tgtagagaat
       61 gctaatgatt agccctggat gggtaaaatg gaggagtttt cagaggcgac aaacaatata
      121 aatgagtaga agtctttaca ggttccaccc atttataacg cttataaatg tttaatcagg
      181 caatatttag atatttatgt gtttgcatcg acaaacgcca attacgcgca atgcaggttt
      241 caatgcacaa acgtgtggca tcatcctttt ggctaatcgg ctgcagtgca atgactcgcg
      301 gtttatcatc ggtcagcgtt gccaatagtt catccagtgc ttcaatatcg cgtacgcgcc
      361 ccactggatg cttgatttcg ttggctcgct ccagtgcctg tgacaacacg tcatagccgc
      421 cgcgcatgtt cagctttggc ga
//
LOCUS       DAJVRF010000420          442 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJVRF010000420 DAJVRF010000000
VERSION     DAJVRF010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.425"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..155)
                     /gene="ortT"
                     /locus_tag="OQN13_004272"
     CDS             complement(<1..155)
                     /gene="ortT"
                     /locus_tag="OQN13_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCS1617433.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVAL"
     gene            350..>442
                     /gene="yncL"
                     /locus_tag="OQN13_004273"
     CDS             350..>442
                     /gene="yncL"
                     /locus_tag="OQN13_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCS1617434.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          136 a           87 c           95 g          124 t
ORIGIN      
        1 agcgccaccg ggaaactcat cggccatgtt gcccccacca gaaaggcgct taagaaacgg
       61 atgcgtttct tatcgtgaga aagaaaccag gtgataagaa atgcgattgc tgccataacc
      121 gcataaaaaa caagcatatg ttgatagaga gacatgtacg ggcgtccagc cagatgaata
      181 aaacgcgcgc aaatatgcgt cattttttga cgtgcatcaa gttttttcac cagtttgaga
      241 aaaaatcatc aaacagcaac ttgcccttat ccatcttcag aatgagtatt attgcggatt
      301 ttcttaaccc gtactataca cataggggca atgataaaag gtggcaaaaa tgaatgtttc
      361 cagtagaact gtagtactga taaatgtctt tgctgctgtt ggtttgttta ctcttatctc
      421 tatgagattt ggctggttta tt
//
LOCUS       DAJVRF010000421          441 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.426, whole genome shotgun sequence.
ACCESSION   DAJVRF010000421 DAJVRF010000000
VERSION     DAJVRF010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.426"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..315
                     /locus_tag="OQN13_004274"
     CDS             <1..315
                     /locus_tag="OQN13_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="similar to the fimbrial usher protein PapC; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HCS1617435.1"
                     /translation="RQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNVI
                     GNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSIILNGIRCQ
                     IQ"
     gene            332..>441
                     /locus_tag="OQN13_004275"
     CDS             332..>441
                     /locus_tag="OQN13_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1617436.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKV"
BASE COUNT          152 a           73 c           98 g          118 t
ORIGIN      
        1 cgtcagggtt cagtcgtctt tgctgatttt gaaaccgtgc aagggcaatc agccattatg
       61 aacatcacac gaagtgatgg taaaaatatt ccatttgctg cagatattta tgatgagcaa
      121 ggcaatgtca ttggtaatgt tggacagggt ggacaagcat ttgttcgtgg cattgagcag
      181 cagggaaata tcagcattaa atggctcgaa gaaagtaaac ccgtaagttg tcttgcgcat
      241 tatcaacaaa gttcagaagc agaaaaaata gcgcaatcta ttattctgaa tggaattcgg
      301 tgtcagattc agtaactaca aggaacgtca aatgataaaa ataacgccac ataaaatagc
      361 gatactgatg ggaatattat tatcaccatc agtatttgca acggatatta atgtagagtt
      421 tacagccact gtcaaagtag a
//
LOCUS       DAJVRF010000422          440 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.427, whole genome shotgun sequence.
ACCESSION   DAJVRF010000422 DAJVRF010000000
VERSION     DAJVRF010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.427"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>440
                     /locus_tag="OQN13_004276"
     CDS             <1..>440
                     /locus_tag="OQN13_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecQ"
                     /protein_id="HCS1617437.1"
                     /translation="SPDIDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLP
                     AGLQGSTGRVLSRWGDSGWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVC
                     CVPSLKHLDLVPDFARRLAAKLGLPFIDAIEKVVDNPPQKMQQN"
BASE COUNT          115 a          101 c          118 g          106 t
ORIGIN      
        1 gtcgcccgat attgatagcg gcttactcca tgcggcaaat ttatttatta aacacgctga
       61 cctgccatta aatctcaata aacaggtagc atctggagct tttacgcaat acggatttaa
      121 agggaacttg cctgcgggtt tacaaggatc tacggggcga gtcctttctc gttggggaga
      181 ttccgggtgg ggaaagcagg tagcacagga gaaaaaaacg gggcgattca gtgatgagct
      241 ggtggaggca tgtgcggaaa tggttcgcca acgctggaat ccgcatcctg aaccaacctg
      301 ggtatgctgc gttccttcat taaagcacct cgacctggtt cctgattttg cccggcgact
      361 ggcggcgaaa cttggcttac cttttattga tgccattgaa aaagtcgtgg acaatccacc
      421 gcagaaaatg cagcaaaacc
//
LOCUS       DAJVRF010000423          437 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.428, whole genome shotgun sequence.
ACCESSION   DAJVRF010000423 DAJVRF010000000
VERSION     DAJVRF010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.428"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            106..>437
                     /locus_tag="OQN13_004277"
     CDS             106..>437
                     /locus_tag="OQN13_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617438.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISLAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSS"
BASE COUNT          113 a           91 c           94 g          139 t
ORIGIN      
        1 aatgcagtaa ctatattttc ctttctggtg ataacgtcgg gttatcatta gcttcttcag
       61 ctattttact tttatatccc ttgtaaacat catggagaat ccatagtgaa taaagtttat
      121 tctcttaaat attgccccgt caccgggggg cttattgctg tctctgaact tgcccgcagg
      181 gtaataaaaa agacatgccg aagattaacg catattcttc tggctggcat tccagcaatc
      241 tgtctgtgtt actctcagat atccctggcg ggtattgtcc gttccgatat tgcctatcag
      301 atttatcgtg atttcgccga aaacaaaggg ctttttgtac ctggtgccaa tgatattccg
      361 gtatatgata aggacggaaa acttgtggga agactgggta aagccccaat ggccgatttc
      421 agcagtgtga gctcaaa
//
LOCUS       DAJVRF010000424          435 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.429, whole genome shotgun sequence.
ACCESSION   DAJVRF010000424 DAJVRF010000000
VERSION     DAJVRF010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.429"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            89..>435
                     /locus_tag="OQN13_004278"
     CDS             89..>435
                     /locus_tag="OQN13_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCS1617439.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIG"
BASE COUNT          104 a          104 c          127 g          100 t
ORIGIN      
        1 cccggtggtg atgtctatct cacaccgatg aacatgacca cgaaaccctc cgatggcagt
       61 aaggccggta agcagaagga taacgccaat gcagacgaaa caacgtcttg atgtaccgct
      121 gagtctgaaa tctgtcagtg actccggtga gtttgaaggg tatggctccg tctttggtgt
      181 aaaggacagc cacgatgatg tggtgatgtc cggggcattt gctgcttccc tgcgggcgtg
      241 gagtgacaga aaagcgttac ctgcgctgct ctggcagcac cgcatggatg aacccatcgg
      301 tgtttacacc gaaatgaagg aagacgatgt cgggctttac gtcaggggac ggttgcttat
      361 tgatgatgat cccctcgcaa aacgtgcaca tgcacacatg aaggccggtt cgttaaccgg
      421 cctttctatt gggta
//
LOCUS       DAJVRF010000425          435 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.430, whole genome shotgun sequence.
ACCESSION   DAJVRF010000425 DAJVRF010000000
VERSION     DAJVRF010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.430"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..172
                     /locus_tag="OQN13_004279"
     CDS             <1..172
                     /locus_tag="OQN13_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617440.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            185..>435
                     /locus_tag="OQN13_004280"
     CDS             185..>435
                     /locus_tag="OQN13_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCS1617441.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFR"
BASE COUNT          111 a           82 c          121 g          121 t
ORIGIN      
        1 ggcgaaagcc ttaatggcgc aatatcgggt accggtagtc gtggaagtta ttcttgagcg
       61 tgtgaccaat atttcgatgg gcagcgaact ggataacgtc atggaatttg aagatatcgc
      121 cgataacgca gtggacgcac cgactgaaac ctgcttcatg cactatgaat aagggagata
      181 aataatgtta cgtttctctg ctaatttatc gatgttattt ggtgaatatg attttctcgc
      241 ccgttttgag aaagctgcgc agtgtggttt tcgcggcgtt gaatttatgt ttccttatga
      301 ctacgacatt gaagaattaa aacaggtgct ggcgagtaat aaactcgaac atacgctgca
      361 caatctaccg gcgggtgact gggcggcggg ggagcgcggt attgcctgta ttcctggccg
      421 tgaagaagag tttcg
//
LOCUS       DAJVRF010000426          432 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.431, whole genome shotgun sequence.
ACCESSION   DAJVRF010000426 DAJVRF010000000
VERSION     DAJVRF010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.431"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            101..166
                     /locus_tag="OQN13_004281"
                     /pseudo
     CDS             101..166
                     /locus_tag="OQN13_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
BASE COUNT          138 a           94 c           94 g          106 t
ORIGIN      
        1 ataaaatcac cctatagatg cacaaaaaac gggcaaaact acctggttcg caaaactgcg
       61 tctaaagtta aaccgggacc tcgcgagcaa gggtgagacg atggcgcttt acacaattgg
      121 tgaagtggcg ttgctttgtg atattaaccc tgtcacgtta cgcgcgtgac gcaacatttt
      181 taaaaatcga cgaagatcgt ccaggagcgc cgtttttcaa gggttggata gatgattcgt
      241 ttaccatgtc gacgaacttc gacgaatttc gccgataaaa agtgtcccat acatgcccca
      301 ttgatgcccc ataaacaata tcaggaattg acactggtta tccatacaga taaaaataac
      361 acctgtacat aaccacagtt acaggagggt gttatgcgtg ttgagatctg tatcgctaaa
      421 gaaaaaatca ct
//
LOCUS       DAJVRF010000427          431 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.432, whole genome shotgun sequence.
ACCESSION   DAJVRF010000427 DAJVRF010000000
VERSION     DAJVRF010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.432"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..327)
                     /locus_tag="OQN13_004282"
     CDS             complement(<1..327)
                     /locus_tag="OQN13_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCS1617442.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQA"
BASE COUNT          106 a          119 c          100 g          106 t
ORIGIN      
        1 cgcttgctca ttaatcaccc ccagttgcat ccagagggtt ttcgcgccaa tggcaatagc
       61 ttcctgtgcc acgccccacg ccgcttctga attacggaaa acatcgacca tatcgacctt
      121 ttccggaacg tctgccagcg tgccataccc ctgctggccc agcagcgttt tgccggcaac
      181 ttttggcgaa accggaatga cgtgataccc ctggtcgagt agatatttca tcacgcgata
      241 gctgggacga tcgggtttgt cgcttgcccc caccagcgca atggtgtgag tggacgttaa
      301 aatgcctgca atatcggttt ctttcattat ttcctccaga ctgtttaaca aagtgtacga
      361 caaagccgac atgccaacca tgcagctaat acgaatgaat gagagcaaaa gcgggaaata
      421 tgtctatatg t
//
LOCUS       DAJVRF010000428          430 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.433, whole genome shotgun sequence.
ACCESSION   DAJVRF010000428 DAJVRF010000000
VERSION     DAJVRF010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.433"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..411)
                     /locus_tag="OQN13_004283"
     CDS             complement(<1..411)
                     /locus_tag="OQN13_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1617443.1"
                     /translation="MQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNN
                     GGYGAKPSKPPTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCR
                     KWVKPNLTCMPQDDPQYPGMETKTASDYLPQTNWS"
BASE COUNT          100 a          101 c           99 g          130 t
ORIGIN      
        1 tgaccaattt gtctgtggca ggtaatcact tgctgtcttt gtctccatcc ctggatactg
       61 gggatcatcc tgcggcatac aagtcagatt gggttttacc cacttcctgc aatacagtgt
      121 gtaatcgtaa gggcttaatg ctctgaagaa tgttttgaaa acattaggcg cactactggc
      181 ctggtcaata ggatatggca gtcttaattg tttcgctatg tctggcaact tacttggatc
      241 gggcgtaggc ggtttgctgg gtttcgctcc atatccgcca ttatttaagt gtccatattt
      301 agatgccagc cccattgaat ctatatactg aaccggattc aacggatact gataaaaatt
      361 ccatcccccc ttcagtccaa tcggatcctg agtgatatac cgcccctgca gcgggtcata
      421 atagcggtgg
//
LOCUS       DAJVRF010000429          422 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.434, whole genome shotgun sequence.
ACCESSION   DAJVRF010000429 DAJVRF010000000
VERSION     DAJVRF010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.434"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            13..>422
                     /gene="soxR"
                     /locus_tag="OQN13_004284"
     CDS             13..>422
                     /gene="soxR"
                     /locus_tag="OQN13_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCS1617444.1"
                     /translation="MAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYVAII
                     KIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDELDG
                     CIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDE"
BASE COUNT           98 a           92 c          134 g           98 t
ORIGIN      
        1 acccccggcg aagtggcgaa acgcagcggt gtggcggtat cggcgctgca tttctatgaa
       61 agtaaagggt tgattaccag tatccgtaac agcggcaatc agcggcgata taaacgtgat
      121 gtgttgcgat atgttgcaat tatcaaaatt gctcagcgta ttggcattcc gctggcgacc
      181 attggtgaag cgtttggcgt gttgcccgaa gggcatacgt taagtgcgaa agagtggaaa
      241 cagctttcgt cccaatggcg agaagagttg gatcggcgca ttcatacctt agtggcgctg
      301 cgtgacgaac tggacggatg tattggttgt ggctgccttt cgcgcagtga ttgcccgttg
      361 cgtaacccgg gcgaccgctt aggagaagaa ggtaccggcg cacgcttgct ggaagatgaa
      421 ca
//
LOCUS       DAJVRF010000430          420 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.435, whole genome shotgun sequence.
ACCESSION   DAJVRF010000430 DAJVRF010000000
VERSION     DAJVRF010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.435"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
BASE COUNT          135 a           69 c           58 g          158 t
ORIGIN      
        1 ttcattactg ctcctgaacg gtataaagga gtaagtgtag acgatggtaa atgaatttta
       61 tggggcagag tttttgcggg gaagtatttt gttggtaaaa atattatcaa cttcatctga
      121 gtgagaatta ttctactatt tcccgagctg cattcatttt ttgtattttt tatttttaga
      181 aaataacagt tctcaaagct taattacctc actattaatt gtatttcaaa ctaacaatcg
      241 cactccattt tctcttttta aaaaacataa acactgttaa aacaaatact tatttcgtta
      301 tcttaaatgc tatttctaca tcactcgtcc ttataatcaa attattctta ttcatagcta
      361 tttttgcagg tttaaaatag cggctttcac caaagtcagc catgaaaatt agactacaag
//
LOCUS       DAJVRF010000431          417 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.436, whole genome shotgun sequence.
ACCESSION   DAJVRF010000431 DAJVRF010000000
VERSION     DAJVRF010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.436"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..140)
                     /locus_tag="OQN13_004285"
     CDS             complement(<1..140)
                     /locus_tag="OQN13_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617445.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAV"
     gene            complement(186..>417)
                     /locus_tag="OQN13_004286"
     CDS             complement(186..>417)
                     /locus_tag="OQN13_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617446.1"
                     /translation="GVLMTATFGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIG
                     VLNAPINAYEMVIYPIAFFVCLFGQMRLFRSV"
BASE COUNT          142 a           89 c           78 g          108 t
ORIGIN      
        1 acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag ccctaacaga
       61 taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc cggtgaatag
      121 gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta agagctttat
      181 gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa aaaggcgatg
      241 gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat aaacaagaga
      301 ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc cagttgcccg
      361 agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa cacgcca
//
LOCUS       DAJVRF010000432          417 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.437, whole genome shotgun sequence.
ACCESSION   DAJVRF010000432 DAJVRF010000000
VERSION     DAJVRF010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.437"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..>417)
                     /locus_tag="OQN13_004287"
     CDS             complement(<1..>417)
                     /locus_tag="OQN13_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endochitinase"
                     /protein_id="HCS1617447.1"
                     /translation="GNKYQVIFDSKIYENAWWGFSTNCPGKAKANDATNPWRLKRTAT
                     AAEISQFGNTLSCEKSGSSSSSNSNTPASNTPANGGSATPAQGTVPSNSSVVAWNKQQ
                     GGQTWYVVFNGAVYKNAWWVASSNCPGDAKGNDASNP"
BASE COUNT          108 a          101 c           92 g          116 t
ORIGIN      
        1 tgggttgctg gcatcattgc ctttcgcatc acccggacag ttagaagagg ctacccacca
       61 agcattttta tataccgcac cattaaagac gacataccag gtctgaccgc cctgctgttt
      121 attccaggca actacagaag aattagacgg aacagtgccc tgtgctggtg tagccgaacc
      181 gccattagcc ggcgtattgg atgcaggcgt atttgaattt gaagaagatg agctgccgct
      241 cttttcgcag gaaagtgtat tgccaaactg actaatttca gcagctgttg cggtacgctt
      301 taaacgccac gggttagttg catcatttgc tttcgctttt cccgggcaat ttgtagaaaa
      361 accccaccag gcattttcat aaattttgct atcgaaaata acctgatatt tattacc
//
LOCUS       DAJVRF010000433          413 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.439, whole genome shotgun sequence.
ACCESSION   DAJVRF010000433 DAJVRF010000000
VERSION     DAJVRF010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.439"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            1..413
                     /locus_tag="OQN13_004288"
                     /pseudo
     CDS             1..413
                     /locus_tag="OQN13_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          123 a           93 c          107 g           90 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac gga
//
LOCUS       DAJVRF010000434          408 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.440, whole genome shotgun sequence.
ACCESSION   DAJVRF010000434 DAJVRF010000000
VERSION     DAJVRF010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.440"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            54..130
                     /locus_tag="OQN13_004289"
     tRNA            54..130
                     /locus_tag="OQN13_004289"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="OQN13_004290"
     tRNA            173..248
                     /locus_tag="OQN13_004290"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT          115 a           86 c           97 g          110 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcg
//
LOCUS       DAJVRF010000435          402 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.441, whole genome shotgun sequence.
ACCESSION   DAJVRF010000435 DAJVRF010000000
VERSION     DAJVRF010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.441"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(163..>402)
                     /locus_tag="OQN13_004291"
     CDS             complement(163..>402)
                     /locus_tag="OQN13_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617448.1"
                     /translation="DLDKLLKLMAENSEEIEDFIQEIETGEPRFMFPVTDSNMKRLII
                     EETNKYSVSPEKYLKAAIAILYSDNILVTDSKVVH"
BASE COUNT          130 a           83 c           60 g          129 t
ORIGIN      
        1 ttgaaagaaa aaataagaga gaattgctct cccccatata tacaattatg tcatgctcaa
       61 ggttgtttgt caacagcctt taacagattg gtaacattta tactttaata gatgcacctg
      121 caatcatacc aatcaataca aaacagataa tcaccaattc cattaatgca ccaccttcga
      181 atccgtcacc agaatattat cgctatacaa aattgctata gccgccttga gatatttttc
      241 agggctaacg ctgtatttgt tcgtttcttc gataataaga cgcttcatgt ttgagtcggt
      301 aacaggaaac ataaaacgcg gttcaccagt ttcaatttcc tgaataaagt cttcaatttc
      361 ttctgagttt tcagccatta gctttagtaa tttatcaagg tc
//
LOCUS       DAJVRF010000436          397 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.442, whole genome shotgun sequence.
ACCESSION   DAJVRF010000436 DAJVRF010000000
VERSION     DAJVRF010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.442"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..>397)
                     /locus_tag="OQN13_004292"
     CDS             complement(<1..>397)
                     /locus_tag="OQN13_004292"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="catalyzes the interconversion of D-xylose to
                     D-xylulose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCS1617449.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEE"
BASE COUNT          106 a          111 c           91 g           89 t
ORIGIN      
        1 ctctcttctt gcttggctgc caggacatca accatttgcg caaaattatt gatgtactct
       61 tttaacgacg cgccctcagg ggaaacatcc acatcgtgga agcaataaaa tggcacatgt
      121 aacttgtgga aaaactcaaa tgcgacatct gctttacgct tcgccaacgc cagtgcctca
      181 ccaggctgct gccacggacg attaaacgcc cccacaccaa acatgtccgc cccgttccag
      241 cagaaggtgt gccagtagca ggcggcaaaa cgcaagtgct cttccatacg cttacccaac
      301 accagttcgt cgggattgta gtgacggaat gctaacgggt ttgaggattt tgagccttca
      361 taacgaacgc gatcgagctg gtcaaaatag gcttgca
//
LOCUS       DAJVRF010000437          396 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.443, whole genome shotgun sequence.
ACCESSION   DAJVRF010000437 DAJVRF010000000
VERSION     DAJVRF010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.443"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            20..196
                     /locus_tag="OQN13_004293"
     CDS             20..196
                     /locus_tag="OQN13_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617450.1"
                     /translation="MSFIFNEETLYFTVFGGDFIMDDLKIVKNTEAWVWYKNCDSKNK
                     KVAVELLQIYELHQ"
BASE COUNT          145 a           44 c           89 g          118 t
ORIGIN      
        1 aataaagtaa cggggggcag tgagttttat ttttaatgag gaaacattgt attttactgt
       61 ttttggtggg gattttatta tggatgattt aaaaatagta aaaaatacgg aagcatgggt
      121 atggtataaa aattgcgata gtaaaaataa aaaagtggca gttgaacttc ttcaaatcta
      181 cgaattgcat caatgaaaaa tatagttaaa tagaggaggt tattaatagc aggaaaagca
      241 tcacgtgatg ctgatttttt gcactaaata attcgcgcta atgttcaata gttaatatat
      301 ccctcatcag ggaaagatca cggtgtgccg taggcacaaa gatgcaaaac gggacgtttt
      361 gcatgtaaca tgtaataagc ataagcttgt gaagag
//
LOCUS       DAJVRF010000438          396 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.444, whole genome shotgun sequence.
ACCESSION   DAJVRF010000438 DAJVRF010000000
VERSION     DAJVRF010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.444"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..233
                     /locus_tag="OQN13_004294"
     CDS             <1..233
                     /locus_tag="OQN13_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617451.1"
                     /translation="ETDAPIKQALAHGLLLHLKNIYPVFPESLVMLLLSVLDVKTYRD
                     DARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
BASE COUNT          118 a           84 c           80 g          114 t
ORIGIN      
        1 cggagacaga cgcgcccatt aagcaggcgc ttgcgcatgg attattatta cacttaaaaa
       61 atatctaccc tgtttttcct gaatcactgg tgatgttatt gctctctgtt ctggacgtaa
      121 aaacatacag agatgatgca cgattaaatg aatggatatc taatagagtg caggaattag
      181 gtgacagata ttatcctgta aataagcacg ttaaaatccg ctacactctt tagttcagaa
      241 agggttccac ctgcaagttg ttattccacc agagagtgaa tcgcaaagca ggtaatcatt
      301 tcatctgtat atccgtatgc caaatggcat tccaccgcct tttcgccgat attttgttgt
      361 gatcggcgac acttcgctta aaaaagcacc agcagt
//
LOCUS       DAJVRF010000439          395 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.445, whole genome shotgun sequence.
ACCESSION   DAJVRF010000439 DAJVRF010000000
VERSION     DAJVRF010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.445"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(24..>395)
                     /locus_tag="OQN13_004295"
     CDS             complement(24..>395)
                     /locus_tag="OQN13_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCS1617452.1"
                     /translation="FQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHG
                     WQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGD
                     CQGYLFRCLHCGKLRLWGDFS"
BASE COUNT          118 a          108 c           62 g          107 t
ORIGIN      
        1 gcctgttctc attatttaaa taactacgaa aaatcacccc acagtctcag cttgccgcag
       61 tggagacagc ggaagagata accctgacaa tcaccaccct tttgcaggca tttagctaga
      121 tcagaatttc taataccgaa attctcacaa tcttcttcaa ggttggcaaa ttcatcgagg
      181 cgatctttta tatcattcca gcccacatag ccgataaaag cacagaaatc gccacaatgc
      241 gcaagccaga attcctgttg ccatccatga taacctggcg tgcgttcaac caactctttc
      301 agcatttcat caggatatgt gttcttaata ccggcaaatt cgtcctcttc atcatactca
      361 aattcaacac cttctatgct ggcatcatcc tgaaa
//
LOCUS       DAJVRF010000440          395 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.446, whole genome shotgun sequence.
ACCESSION   DAJVRF010000440 DAJVRF010000000
VERSION     DAJVRF010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.446"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>395
                     /gene="curA"
                     /locus_tag="OQN13_004296"
     CDS             <1..>395
                     /gene="curA"
                     /locus_tag="OQN13_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCS1617453.1"
                     /translation="GDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETL
                     VVAAATGPVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQL
                     VKACPKGIDIYYENVGGKVFDAVLPLLNTS"
BASE COUNT           89 a           77 c          131 g           98 t
ORIGIN      
        1 ggtgatgatc tggtgaaact tggcgatcat ccgcaaaatc catcgtggtc gctgggtgtg
       61 ctggggatgc caggctttac cgcttatatg gggctgctgg atatcggtca gcctaaagag
      121 ggcgaaacgt tggtggtagc tgcggcgaca ggtcctgtgg gagcaacggt ggggcaaatc
      181 ggcaaactta aaggttgcag ggtggtgggg gttgccggtg gcgcggaaaa atgccgccat
      241 gctatcgagg tgctgggctt cgatgtttgt cttgatcacc acgcggatga ttttgccgaa
      301 caactggtga aagcgtgccc aaaaggtatt gatatctatt atgaaaacgt gggcggtaag
      361 gtatttgatg cagtgctacc attacttaat acatc
//
LOCUS       DAJVRF010000441          395 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.447, whole genome shotgun sequence.
ACCESSION   DAJVRF010000441 DAJVRF010000000
VERSION     DAJVRF010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.447"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
BASE COUNT          133 a          105 c           53 g          104 t
ORIGIN      
        1 tggcacaata acatcaacca gcgactcttc aaaccaacac ccaaatgcat ttctttatga
       61 attaatccgt aacaaacacg cttcccatat agaaatattt gcaaaaatag tgcatgaaat
      121 aatcttttct ctaccaatcc gcgatataaa tattcatcag taccacaata tttcacagca
      181 ctaaatctct ccccgccgcc ccgtaccttt gataatggcc taaaatcatt gaggccactt
      241 gcgacgacgt tgctcaataa ggaaaatttg gcgtgaataa tattcaacac cagtgcagca
      301 tcatcattac atacttacac ccgccgacta ccgctttcga tatattgact caattttcca
      361 gactggttaa aaatagcgac cagctcagtc gcaaa
//
LOCUS       DAJVRF010000442          392 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.448, whole genome shotgun sequence.
ACCESSION   DAJVRF010000442 DAJVRF010000000
VERSION     DAJVRF010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.448"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..>392)
                     /locus_tag="OQN13_004297"
     CDS             complement(<1..>392)
                     /locus_tag="OQN13_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS1617454.1"
                     /translation="EVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVRE
                     AGVFLMDEPLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKD
                     GIVQQVGAPKTVYNQPANMFVAGFIGSSA"
BASE COUNT           82 a          126 c           94 g           90 t
ORIGIN      
        1 gccgatgatc caataaatcc ggcaacaaac atattcgctg gttggttata aaccgttttc
       61 ggcgcaccaa cttgctgaac aatcccatcc ttcataatca caatccgcgt cgccatggtc
      121 atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg tattcagctt ctggtgcagc
      181 ttgctgatct ctgcgcgcat ttgcacgcgc agcttggcat caaggttaga gagcggttca
      241 tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc cgagcgccac acgctggcgt
      301 tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact cacgcaggcc gagaatttgc
      361 gccgcccagt tcacccgctc atcaatcacc tc
//
LOCUS       DAJVRF010000443          391 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.449, whole genome shotgun sequence.
ACCESSION   DAJVRF010000443 DAJVRF010000000
VERSION     DAJVRF010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.449"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            47..>391
                     /locus_tag="OQN13_004298"
     CDS             47..>391
                     /locus_tag="OQN13_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1617455.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRIL"
BASE COUNT          103 a           82 c          102 g          104 t
ORIGIN      
        1 ttatttttat gattaaggat aatcaataat taaaacagga agttctatgt ctacgagaag
       61 agaagttatt ctttcctggt tgcgtgagaa acgtcagacc tggcgtctgt gctatttgtt
      121 gggtgaggct ggaagtggaa aaacctggct ggcgcagcaa ctgcaaaaag ataaacatcg
      181 ccgtgtgatt actttaagcc tcgttgtttc ctggcaaggt aaggccgcat ggatcgttac
      241 cgacgataac gcggctgaac agggctgccg tgacagcgcc tggacgcgag atgagatggc
      301 ggggcaatta ctgcatgcgc ttcatcgaac tgatagtcgc tgccctctta ttattattga
      361 aaatgctcac ctgaatcacc gccgcatact t
//
LOCUS       DAJVRF010000444          389 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.450, whole genome shotgun sequence.
ACCESSION   DAJVRF010000444 DAJVRF010000000
VERSION     DAJVRF010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.450"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..>389)
                     /locus_tag="OQN13_004299"
     CDS             complement(<1..>389)
                     /locus_tag="OQN13_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCS1617456.1"
                     /translation="MPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWL
                     VRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVL
                     PAPLPPYRVLTGLVDRFGRTQTFHREA"
BASE COUNT           71 a          134 c          114 g           70 t
ORIGIN      
        1 cggcttcgcg gtggaacgtc tgtgtgcgcc cgaagcggtc caccagcccg gtcagtaccc
       61 ggtacggcgg cagcggcgca ggcagcacct catccgcttc cggcacccgc tcacaccagc
      121 cgagcagcca ccacggcccc tgcggactgt ttgtcgccag ataacgatgc ggacttaagc
      181 ggagttcttc cggcagcgcc tgccagagtg cggccagccg gtgaccttca tccagtttcg
      241 ccacgccgcc gcgcaccagc cagagtgatt cgctgcggct gtaaccgtcc tcaccgggaa
      301 acaggtgctc aaaatacagg cttctgccgc cgttatcact gagtatcagt gtgttatcgc
      361 gcagctgtaa gcggatatcc gcaggcatt
//
LOCUS       DAJVRF010000445          389 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.451, whole genome shotgun sequence.
ACCESSION   DAJVRF010000445 DAJVRF010000000
VERSION     DAJVRF010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.451"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            24..>389
                     /locus_tag="OQN13_004300"
     CDS             24..>389
                     /locus_tag="OQN13_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1617457.1"
                     /translation="MLIVSPSVVASEKAHELYDSIYGGKPAPDVINTLHKMAESGDID
                     AQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYDGEQVRVDYAK
                     AYALFNQAAQHGVNLAWSRL"
BASE COUNT          109 a           74 c          109 g           97 t
ORIGIN      
        1 ctaaaagaat attattttct gtaatgctga tagtttcacc aagcgtggtg gccagcgaga
       61 aagcgcatga attgtatgac agcatatacg gtggtaaacc tgctcccgat gttataaata
      121 cactgcataa aatggctgaa tcgggagata tcgacgcgca gagcctgtta ggctgggaat
      181 attatcagcc tcgttatgat accaaacctg atgttcagga agcgattaaa tggttcgagt
      241 tagcggctaa acaaggtgac agagaagctc cgttagcgct ggggggtatc tactacgacg
      301 gtgagcaggt gcgggtggat tatgccaaag cgtatgcact atttaatcag gccgcgcagc
      361 acggtgtgaa tttagcatgg tccaggttg
//
LOCUS       DAJVRF010000446          389 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.452, whole genome shotgun sequence.
ACCESSION   DAJVRF010000446 DAJVRF010000000
VERSION     DAJVRF010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.452"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..353)
                     /locus_tag="OQN13_004301"
     CDS             complement(<1..353)
                     /locus_tag="OQN13_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105657.1"
                     /note="phosphorylates aminoimidazole ribotide (AIR) which
                     forms 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate
                     (HMP-P); synthesis of HMP-P from AIR is complex and
                     requires the product of thiC gene at least; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HCS1617458.1"
                     /translation="MPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDDPFGAL
                     MQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETTDLPCW
                     RHGEWLHLCSIALSA"
BASE COUNT          102 a          117 c           85 g           85 t
ORIGIN      
        1 tcggcagaca acgcaattga acagagatgt aaccattcgc catgtcgcca gcagggcaag
       61 tctgtcgtct ctaaaaaaag atcggcactg gggcggacca taaacgtaaa tgaacgttcc
      121 ccttgatcgt tcagatcgac aagcaccgtg gatgtccggt gccattcatc ttgcttcaga
      181 tacgtgatat cgactccctc agttagcagc gttctttgca ttaacgcccc aaaaggatca
      241 tcaccgaccc gacctataaa tccacttatt ccgcctaatc tggcgattcc caccgcaacg
      301 ttagctggcg cgccgccagg acaaggcagt aggcgcccgt ctgattctgg caagagatct
      361 acgaccgcat cccctaaaac ccatacttt
//
LOCUS       DAJVRF010000447          387 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.453, whole genome shotgun sequence.
ACCESSION   DAJVRF010000447 DAJVRF010000000
VERSION     DAJVRF010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.453"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..360
                     /locus_tag="OQN13_004302"
     CDS             <1..360
                     /locus_tag="OQN13_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCS1617459.1"
                     /translation="YAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYVYSK
                     GKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDFYDN
                     NGIATDDVVGVGLVYQF"
BASE COUNT          118 a           76 c           83 g          110 t
ORIGIN      
        1 tatgctgaaa cccgcaatat gacaccatat ggtaatgtgg gtatcgcaaa taaaacccag
       61 aactttgaag ctgtagcaca gtatcagttc gattttggtc tgcgcccatc tcttgcatat
      121 gtttattcaa aaggcaaaga cctcggcggt aacgactata acaacaatgg tcatcaggag
      181 tatgttgatc aggatctggt caactatgtt gaaattggag cgacctacta tttcaataag
      241 aacttctcaa catatgttga ctataaaata aacttgttgg ataaagatga cgatttctat
      301 gacaacaatg gcatcgcaac agatgacgtt gttggtgtgg gcttagtcta ccagttctaa
      361 tcagccaacg tgctgcggcg tgttact
//
LOCUS       DAJVRF010000448          386 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.454, whole genome shotgun sequence.
ACCESSION   DAJVRF010000448 DAJVRF010000000
VERSION     DAJVRF010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.454"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..>386)
                     /locus_tag="OQN13_004303"
     CDS             complement(<1..>386)
                     /locus_tag="OQN13_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HCS1617460.1"
                     /translation="WLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACS
                     VSQRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYK
                     VREDVMDQIVQEGGLEYATDVIIHLQ"
BASE COUNT           93 a           88 c           94 g          111 t
ORIGIN      
        1 tgttgaaggt gtattattac atcagtagca tattccagac cgccttcctg gacgatctga
       61 tccataacat cctcacgcac tttatatctt gtgcatttca tttcaagtgc cgtgagtaca
      121 gccattactg gtgatggtaa ctgtgttgtt ttcaggccgt cgatctgact gatcgcctgg
      181 gcgacggtgt tttgccattc taaagaacag gcgctgaagt caaatgtggc tgttttatta
      241 tcccgttgcg acacactaca ggctaagcga cgtaattggg tcaatttgtc tgcttgctga
      301 acaggaactc cgggaaagcg gcgatgactt aacgtggtgt gtacaacttc gggcggaaaa
      361 acgacagcaa tatctttcgc cagcca
//
LOCUS       DAJVRF010000449          381 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.456, whole genome shotgun sequence.
ACCESSION   DAJVRF010000449 DAJVRF010000000
VERSION     DAJVRF010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.456"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..369
                     /locus_tag="OQN13_004304"
     CDS             <1..369
                     /locus_tag="OQN13_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS1617461.1"
                     /translation="KSGLLKEIICKALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRW
                     GDILSSGEKQRIALARLILRRPKWIFLDETTSHLEEQEAIRLLRLVREKLPTSGVIMV
                     THQPGVWNLADDICDISTVL"
BASE COUNT          110 a           83 c           89 g           99 t
ORIGIN      
        1 aaaagcggct tactgaaaga gattatttgt aaagcacttc ccctgcccgt agacgataaa
       61 tcgttgagcg aagtactgca tcaggttggt ctggggaaat tggctgcgcg tattcatgac
      121 cacgatcgct ggggagatat tctttccagc ggcgaaaaac aacgtatcgc cctggcacga
      181 ttaattttac gacgtccgaa atggatattt cttgacgaaa ctacctctca tcttgaggaa
      241 caagaggcta tacgcttact gcgtttagtg cgtgaaaaac tacccacaag cggcgtcatt
      301 atggttacac atcaacccgg tgtctggaac ctggctgatg atatttgtga cattagcacg
      361 gttttataac aacaataaaa g
//
LOCUS       DAJVRF010000450          381 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.457, whole genome shotgun sequence.
ACCESSION   DAJVRF010000450 DAJVRF010000000
VERSION     DAJVRF010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.457"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..>381)
                     /locus_tag="OQN13_004305"
     CDS             complement(<1..>381)
                     /locus_tag="OQN13_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS1617462.1"
                     /translation="VSSSGYSDNARVSYSVNTGYTMNKASKDLSYVGGYASYESPWGT
                     LAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINY
                     GNSTIDRWGYGVTSALSPYHENRIA"
BASE COUNT          101 a          102 c           82 g           96 t
ORIGIN      
        1 cgcgatacgg ttttcatgat aaggagaaag agcgctggtg acaccataac cccatcgatc
       61 gatagtactg ttgccataat ttattcgcgc tccttgagca cctggagcct gaactaccgc
      121 cagtgtatcg gagtcgctaa aactgtcatt actgaaggtc aggccgccgc tatgcaatac
      181 aaaaccaccg tcggtgctga gagacacttg acgactgtta tcgctatttg cagaaactga
      241 acctgccagg gttccccacg gtgattcata gctggcataa cccccaacat aactcaaatc
      301 tttgctggct ttattcatcg tatagccagt atttacgcta taactgacgc gagcgttatc
      361 actatagcca ctgctactga c
//
LOCUS       DAJVRF010000451          381 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.458, whole genome shotgun sequence.
ACCESSION   DAJVRF010000451 DAJVRF010000000
VERSION     DAJVRF010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.458"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..99
                     /locus_tag="OQN13_004306"
     CDS             <1..99
                     /locus_tag="OQN13_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1617463.1"
                     /translation="EASGGELQYAPKVYDEYRKAKRPWKVIHENQA"
     gene            83..381
                     /locus_tag="OQN13_004307"
                     /pseudo
     CDS             83..381
                     /locus_tag="OQN13_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          108 a           87 c          107 g           79 t
ORIGIN      
        1 gaggcatctg gcggggaact tcagtacgcc cccaaagttt atgacgaata tcgtaaggca
       61 aaacgacctt ggaaggtgat tcatgaaaat caggcatgag cacatcgaat cagtgctgtt
      121 gccctggcag ccgaaaaagg gcaggcgtgg gtcgctaacg caattactga agaatatctg
      181 cggcaggggg gcggcgaatt gtctctggta ccaggcaagg actggaacaa tcagcagaat
      241 atctatcacc gttggttgaa aggtgaaacg aaagcgcaaa gggaaaaaat tcagaaactg
      301 atccctgcgg ttctggcaat tcttccgcgt gagctgcgtc accgactctg tatcttcgat
      361 accctggaac gccgtgcatt a
//
LOCUS       DAJVRF010000452          376 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.460, whole genome shotgun sequence.
ACCESSION   DAJVRF010000452 DAJVRF010000000
VERSION     DAJVRF010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.460"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..356
                     /gene="yahK"
                     /locus_tag="OQN13_004308"
     CDS             <1..356
                     /gene="yahK"
                     /locus_tag="OQN13_004308"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HCS1617464.1"
                     /translation="AHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT          103 a           81 c           91 g          101 t
ORIGIN      
        1 cggctcatct gaagagtttc gatttcattt tgaatacagt agctgcgcca cataatctcg
       61 acgattttac caccttgctg aagcgtgatg gcaccatgac gctggttggt gcgcctgcga
      121 caccgcataa atcgccggaa gttttcaacc tgatcatgaa acgccgtgcg atagccgatt
      181 ctatgattgg cggcattcca gaaactcagg agatgctcga tttttgcgcc gaacatggca
      241 tcgtggctga tatagagatg attcgggccg atcaaattaa tgaagcctat gagcgaatgc
      301 tgcgtggtga tgtgaaatat cgttttgtta tcgataatcg cacactaaca gattgaaaaa
      361 attaataaat acgctg
//
LOCUS       DAJVRF010000453          373 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.461, whole genome shotgun sequence.
ACCESSION   DAJVRF010000453 DAJVRF010000000
VERSION     DAJVRF010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.461"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..301)
                     /locus_tag="OQN13_004309"
     CDS             complement(<1..301)
                     /locus_tag="OQN13_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1617465.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRED"
BASE COUNT          114 a           76 c           79 g          104 t
ORIGIN      
        1 aatcctctct cggcacggtt gcaattagcg agtgggtagt atcaccaaca gcgcgaaatc
       61 cgcgtctgat aatggatatg ttctgatata acgtgttagt aggcacacgc ataccttctt
      121 cttcccagac tctggtgaaa aaatcttgtt gtgcaaccac atcaggaaaa gcttcaagta
      181 gaagtgataa acatcgactg gctggcgttg tcagaacgac gttaagatcg gcgttatgta
      241 ccgatattaa ttttcggtgc tcaggccaga actcgatatt atcgttaata atccagtaca
      301 tacgtcaata aacactaaat caatcgaaat ggagatcaca tagtttgcta agtatattgg
      361 tatttacggc ata
//
LOCUS       DAJVRF010000454          371 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.462, whole genome shotgun sequence.
ACCESSION   DAJVRF010000454 DAJVRF010000000
VERSION     DAJVRF010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.462"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..>371)
                     /locus_tag="OQN13_004310"
     CDS             complement(<1..>371)
                     /locus_tag="OQN13_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCS1617466.1"
                     /translation="TIDYLRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLS
                     SKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQMILSGYTFFPSETLNYIKSNKCST
                     NSSTVTVLSNREVTILRYLVSG"
BASE COUNT          112 a           72 c           75 g          112 t
ORIGIN      
        1 ccgctaacca gataacgtaa tatggtcact tcacgattag atagcacagt gaccgttgaa
       61 ctattcgtac tacatttatt gctttttata tagttgagcg tttcgctagg aaaaaacgtg
      121 tatccggaga ggatcatctg aacgggatga aaaatatcat tctgatcatt gcatttactg
      181 acaaaaccgt tagcaccagc ttgtatcgct ctgccagcat aaaagcattc tgatttcgat
      241 gataaaaata acactttcac tgtgctctgg atttgtttga tccttttcag gaaggtaaaa
      301 ccgtctgttc cgggcaagtc tatatccata atgattaaat caacaggacg ggttcggaga
      361 taatcgatgg t
//
LOCUS       DAJVRF010000455          371 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.463, whole genome shotgun sequence.
ACCESSION   DAJVRF010000455 DAJVRF010000000
VERSION     DAJVRF010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.463"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            259..>371
                     /locus_tag="OQN13_004311"
     CDS             259..>371
                     /locus_tag="OQN13_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCS1617467.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPG"
BASE COUNT          106 a           85 c           99 g           81 t
ORIGIN      
        1 cgccgtatac aacacggcgt ggttataaat ttccgcaatg gcagcacaat cggctttgcg
       61 ggcaaaacgg atggacatgt ctggcctcga taaaataatg atgatgatgt tatcaatact
      121 atcgaaaaca gcccgaagca acggattcct cgatgaaatt attgcgtgtt gcagagggga
      181 tgtgacggct gcaaacaaaa ccaatcccgt gagcctgcta ctattgatgg attcatgcca
      241 aaagcacagg agagcattat ggggcaacaa aagcagcgta atcgacgttg ggttctggcc
      301 tcgcgtccac atggcgcacc ggttccggag aatttccgtc ttgaagaaga tgatgtcgcc
      361 acaccgggtg a
//
LOCUS       DAJVRF010000456          367 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.464, whole genome shotgun sequence.
ACCESSION   DAJVRF010000456 DAJVRF010000000
VERSION     DAJVRF010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.464"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..>367)
                     /locus_tag="OQN13_004312"
     CDS             complement(<1..>367)
                     /locus_tag="OQN13_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCS1617468.1"
                     /translation="IGILVALAVLIAGKPLISLVFGVKYLEAYDLIQVMLGAIVISML
                     GFPQESLLLMAGKQRAFLVAQTIASIGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVL
                     SLIPTLKAFFQRHSLLYNAA"
BASE COUNT          119 a          104 c           82 g           62 t
ORIGIN      
        1 gctgcgttat aaagcaagga atgacgctga aaaaatgcct ttaaagttgg aattaacgag
       61 agcaccacat ccagacattg cccaccgaag taagcaaacg ctgctcccag cacaccaaac
      121 agatgacaga acatgaacag caatacgatg tagccgattg aggcgatggt ttgcgccacg
      181 agaaaagcgc gctgttttcc cgccatcaat agcaatgact cctgcgggaa acccagcatc
      241 gaaatcacaa ttgcgcccag catcacctga atcaggtcat acgcttcgag atacttaacg
      301 ccaaacacca gcgaaatgag cggcttgccg gcgatcaaca ccgcgagcgc caccagaata
      361 ccgattc
//
LOCUS       DAJVRF010000457          362 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.465, whole genome shotgun sequence.
ACCESSION   DAJVRF010000457 DAJVRF010000000
VERSION     DAJVRF010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.465"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            110..>362
                     /locus_tag="OQN13_004313"
     CDS             110..>362
                     /locus_tag="OQN13_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1617469.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAI"
BASE COUNT          123 a           82 c           75 g           82 t
ORIGIN      
        1 gccaaaaaac gctatagcat caattttcaa catatcgagc tccttagatt ttcctaagga
       61 aactagatcg tagagaaacc taagtcaaga aaaattagaa ttccctaata tgaaaaacga
      121 aaccttcggt gctcgcctct tacataggcg taaaaaatta aaactgtctc aggccgcatt
      181 aggtaagctg gtcaaagtgg ctcacgtaac aatttctcaa tgggaaagag atgaaacaca
      241 gccggcgggg aaaagattat tcgcactgag ccaagcgctt cagtgctcac cgacttggct
      301 tctttttggg gatgaagata aacaaccagg cgaaccgatc ccggataatc agccagccat
      361 tc
//
LOCUS       DAJVRF010000458          358 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.466, whole genome shotgun sequence.
ACCESSION   DAJVRF010000458 DAJVRF010000000
VERSION     DAJVRF010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.466"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..246
                     /locus_tag="OQN13_004314"
     CDS             <1..246
                     /locus_tag="OQN13_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1617470.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT           91 a           88 c           88 g           91 t
ORIGIN      
        1 gatgttgatg ctgaactgga tgccgttttg atgggtgctg gtatcgggta tctgctctat
       61 gaacagatca aggagtacct tgataccggt cgtttagaat gtgtactgga agactggatc
      121 acagagcgtc caggcttcca gatctattac ccaaaccgcc agtatatgtc ctgcggcttg
      181 cgcgcatttc tcgattatgt aaaaacgggt cagatctgcc agagtcagcg tcaccgacca
      241 caataattat ttactattcg ctttacgcgc cagcagcggg aatatcagtc ctaatcctac
      301 cagcacaaac ggcgttgcaa cattcagcgc caactggaag gtccactctg cagtgaag
//
LOCUS       DAJVRF010000459          356 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.468, whole genome shotgun sequence.
ACCESSION   DAJVRF010000459 DAJVRF010000000
VERSION     DAJVRF010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.468"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            45..>356
                     /locus_tag="OQN13_004315"
     CDS             45..>356
                     /locus_tag="OQN13_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate oxidoreductase"
                     /protein_id="HCS1617471.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DG"
BASE COUNT           99 a           70 c           84 g          103 t
ORIGIN      
        1 taacaaaaca tatagtttgc catatttata tttaaggaat tgttatggga aataatttgt
       61 tatcagcaaa agcgacactc cctgtttatg atcgtaataa cctggcccca agaattattc
      121 atttaggctt tggtgcattt caccgtgcgc atcagggcgt gtatgccgat attcttgcta
      181 cagaacattt cagtgactgg ggatattatg aggtcaactt aatcggcggc gaacagcaaa
      241 ttgccgattt acaacagcaa gataatcttt ataccgttgc ggaaatgtcg gccgatgcgt
      301 ggacggctcg cgtcgttggc gtcgttaaga aagccttgca cgtacagatg gatggc
//
LOCUS       DAJVRF010000460          353 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.469, whole genome shotgun sequence.
ACCESSION   DAJVRF010000460 DAJVRF010000000
VERSION     DAJVRF010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.469"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>353
                     /locus_tag="OQN13_004316"
     CDS             <1..>353
                     /locus_tag="OQN13_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCS1617472.1"
                     /translation="PGTLNDFLIAPDEGDLKPDVVKRFEEMVAQAQQSAGAAAGNAQQ
                     TAQDVAAAAGYARAAEQAKNDIDAALTGTLKTANHLSEIAAAGEKAQQKSRDNLGLKS
                     AATMEAQSDIYDRTK"
BASE COUNT           97 a           94 c          113 g           49 t
ORIGIN      
        1 agcccggcac gctgaatgac tttctgattg ctcctgatga gggcgacctg aaaccggatg
       61 tcgtcaaacg ctttgaggaa atggtggcgc aggcgcagca gagcgccggg gccgcagccg
      121 gaaacgcaca gcagacggcg caggatgtgg cggcagccgc aggttatgcc cgcgcagcag
      181 agcaggccaa aaatgacatt gatgctgcgc tgaccggcac tctgaaaacg gctaaccatc
      241 tgtctgaaat cgcagcagca ggcgaaaagg cacaacagaa gtcccgggat aatctggggc
      301 tgaaaagtgc ggccacgatg gaagcacaga gcgacattta cgaccggaca aaa
//
LOCUS       DAJVRF010000461          351 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.470, whole genome shotgun sequence.
ACCESSION   DAJVRF010000461 DAJVRF010000000
VERSION     DAJVRF010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.470"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>351
                     /locus_tag="OQN13_004317"
     CDS             <1..>351
                     /locus_tag="OQN13_004317"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCS1617473.1"
                     /translation="IVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDF
                     KRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLP
                     TMVMTPYAAFQKEL"
BASE COUNT           90 a           88 c           83 g           90 t
ORIGIN      
        1 gcatcgtggc gaaatacctc gacgaacatg gcatcgttgt tgagaaaacc ggtccgtata
       61 acctgctgtt cctgttcagc atcggtatcg ataagaccaa agcactgagc ctgctgcgtg
      121 ctctgactga cttcaaacgt gcgttcgacc tgaacctgcg tgtgaaaaac atgctgccgt
      181 ctctgtatcg tgaagatcct gaattctatg aaaacatgcg tattcaggaa ctggctcaga
      241 atatccacaa actgattgtt caccacaatc tgccggatct gatgtatcgc gcatttgaag
      301 tgctgccgac tatggtaatg actccgtatg ctgcgttcca gaaagagctg c
//
LOCUS       DAJVRF010000462          348 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.471, whole genome shotgun sequence.
ACCESSION   DAJVRF010000462 DAJVRF010000000
VERSION     DAJVRF010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.471"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..341
                     /locus_tag="OQN13_004318"
     CDS             <1..341
                     /locus_tag="OQN13_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCS1617474.1"
                     /translation="GHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPET
                     TRCALYSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREI
                     EREVCLWGKQ"
BASE COUNT          105 a           81 c           89 g           73 t
ORIGIN      
        1 acgggcatac aggaaaagac atcattcccg gtaaaacgta taccgaagca gagtgcaaag
       61 ccctcctgaa taaagacctt gccactgtcg ccagacaaat taacccgtac atcaaagtcg
      121 atataccgga aacaacgcgc tgcgctcttt actcgttcgt ctacaacgtg ggtgctggca
      181 atttcagaac atcgacgctt cttcgcaaaa taaaccaggg tgatattaaa ggcgcatgtg
      241 atcagctacg gcgctggaca tacgctggcg gtaatcaatg gaaaggactg atgactcgcc
      301 gtgagattga gcgtgaagtc tgtttgtggg ggaaacaatg agcagagt
//
LOCUS       DAJVRF010000463          343 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.472, whole genome shotgun sequence.
ACCESSION   DAJVRF010000463 DAJVRF010000000
VERSION     DAJVRF010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.472"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
BASE COUNT           95 a           74 c           67 g          107 t
ORIGIN      
        1 ctggtctcgc cattgttcaa ccgtagaaat aaaggtttct acaagttgcc cctgtctggt
       61 taatagaact gaacccgtcg aatcaaccac tacaacaccg ctaatagaga tggtcttagg
      121 tgctctctga acattatcag tgatagtttg ccccgattgc atagcctgag ttgtcaccat
      181 catcggtgag tcatagtcca tctgttcagt agctgttaag gcaagaaaag attccacatt
      241 atctctaagg tgaaaataga taccatcttt acctgagtat ctaatttgct ttgtgtcaaa
      301 cattgttgtt attcctattc tgttaagcgt gagctacgtg aaa
//
LOCUS       DAJVRF010000464          341 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.473, whole genome shotgun sequence.
ACCESSION   DAJVRF010000464 DAJVRF010000000
VERSION     DAJVRF010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.473"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            118..>341
                     /locus_tag="OQN13_004319"
     CDS             118..>341
                     /locus_tag="OQN13_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617475.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVG"
BASE COUNT          107 a           81 c           89 g           64 t
ORIGIN      
        1 acattttctg cctgtggacc aggaaagtgc acgggtgctt tccactccca tgaagcccgg
       61 caatccgcgc agatacagta ataaacgaac tgacagcagt aagatggagc gtttcttttg
      121 aacaaccaga caatgacttt tacccctgaa caattgcgta aacacgcgca ggaaatgctg
      181 cgtcatgctg aacaactcga aaaaacgggc ataacaaaag acgctatccg taaagatatg
      241 gtacccgcgc ttcgggaact gatgcaggcg aagcatcgcg cacagaaagc agtagatgag
      301 ctggtggact gtgtggcaga gctggaaacc agagtcggaa a
//
LOCUS       DAJVRF010000465          341 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.474, whole genome shotgun sequence.
ACCESSION   DAJVRF010000465 DAJVRF010000000
VERSION     DAJVRF010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.474"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..187
                     /locus_tag="OQN13_004320"
     CDS             <1..187
                     /locus_tag="OQN13_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCS1617476.1"
                     /translation="RPVIDRAWDAQLRLCKRYRKLQAKGKNVNITIVAVARELAGFIW
                     DMGRIAMSVAQQPQCHK"
BASE COUNT          102 a           79 c           89 g           71 t
ORIGIN      
        1 ccgccccgtc attgacagag catgggatgc tcaactcagg ctttgtaaga ggtatcgaaa
       61 acttcaggcc aaaggaaaga atgtcaatat tacaattgtt gctgttgcac gtgagctggc
      121 gggttttatc tgggatatgg gccgaatagc aatgtctgtc gcacaacaac cacaatgtca
      181 caaataaaaa atgagaataa ccagttccct gaaggcgacg tagcccggta caagagtaac
      241 ccacgttagc gcttggcgac ggcggaacaa agctgattcg cgacgtaaga aagtggcagg
      301 ctcatatgac ggacctctgt aatgcggtat ccaacccgcg a
//
LOCUS       DAJVRF010000466          332 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.475, whole genome shotgun sequence.
ACCESSION   DAJVRF010000466 DAJVRF010000000
VERSION     DAJVRF010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.475"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            30..>332
                     /locus_tag="OQN13_004321"
     CDS             30..>332
                     /locus_tag="OQN13_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1617477.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQL"
BASE COUNT          118 a           80 c           63 g           71 t
ORIGIN      
        1 caaatataaa aaacacaggt catcagggaa tgccacaacg acaccaccag ggacataaac
       61 gcacaccgaa acagttggcg ctcatcatca aacgttgttt gccgatggtg ctcactggca
      121 gcggcatgct ttgcactacc gctaacgccg aagagtatta tttcgacccc attatgctgg
      181 aaactacaaa aagtggtatg caaacaaccg atctgtcacg tttttcaaaa aaatacgcac
      241 aactaccagg aacttatcag gttgatatct ggctgaataa aaagaaggtt tcacagaaaa
      301 aaattacatt taccgccaat gcagagcaac tt
//
LOCUS       DAJVRF010000467          326 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.476, whole genome shotgun sequence.
ACCESSION   DAJVRF010000467 DAJVRF010000000
VERSION     DAJVRF010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.476"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(<1..>326)
                     /locus_tag="OQN13_004322"
     CDS             complement(<1..>326)
                     /locus_tag="OQN13_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617478.1"
                     /translation="GVTTLLDADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEG
                     LFQATTQTAGKVYVTLGSEGSLWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALA
                     EKMPTK"
BASE COUNT           80 a           90 c           73 g           83 t
ORIGIN      
        1 tcttttgttg gcattttttc tgccaacgct accgcgagtg cgccgtgaaa aacatcgcct
       61 gctccagtgg tatcaaccac attgacagaa aatgcctcct gttgacacaa atgaccatct
      121 tcaatccaca acgatccttc agaacccagc gtaacataca ctttacctgc tgtttgtgtc
      181 gttgcctgga acaaaccttc ttcaggtgac tgcaggccag tcatgcgttt cagccctggt
      241 gttgagaaca cggcgtgatc ggcaagcgcg acaagtggag aaatatcctg cggtgtcata
      301 tcggcgtcta aaagcgttgt cacacc
//
LOCUS       DAJVRF010000468          319 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.477, whole genome shotgun sequence.
ACCESSION   DAJVRF010000468 DAJVRF010000000
VERSION     DAJVRF010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.477"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>319
                     /locus_tag="OQN13_004323"
     CDS             <1..>319
                     /locus_tag="OQN13_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617479.1"
                     /translation="TALFIINFATSPIHAENILITLHYKKEIIPLFLETTPIKANHLR
                     SILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAFFSQSPSDAIFCRHLS
                     LQYA"
BASE COUNT          107 a           75 c           41 g           96 t
ORIGIN      
        1 cactgccctt tttatcatta actttgccac ttcacccatc catgcagaaa atatactcat
       61 tacattacac tataaaaaag aaatcattcc cttatttctg gaaaccacgc ctattaaggc
      121 taaccatttg cgaagcatac tggattatat tgaacaggaa cagttaactg ccgaagatcg
      181 taaccattgt atgaaactgt ctaaaaaaat ccatagagaa aaaactatac aaccaacagt
      241 aaatctcaat ggtagtgcat ttttttcgca atctccttct gacgctattt tttgtcgcca
      301 tctgtcattg caatacgcc
//
LOCUS       DAJVRF010000469          318 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.478, whole genome shotgun sequence.
ACCESSION   DAJVRF010000469 DAJVRF010000000
VERSION     DAJVRF010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.478"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>318
                     /locus_tag="OQN13_004324"
     CDS             <1..>318
                     /locus_tag="OQN13_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HCS1617480.1"
                     /translation="EVARESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSA
                     AGVADQSVTVLTGIKNAVGENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQE
                     MIDE"
BASE COUNT           86 a           72 c           97 g           63 t
ORIGIN      
        1 gaagtggcgc gcgaaagcct ggtgttgctg aaaaaccgtc tcgaaacgtt accgctgaaa
       61 aaatcggcca ccattgcggt ggttgggcca ctggcggaca gtaaacgtga cgtgatgggc
      121 agctggtccg cagccggtgt tgccgatcaa tccgtgaccg tactgaccgg gattaaaaat
      181 gccgtcggtg aaaacggtaa agtgctgtat gccaaagggg cgaacgttac cagtgacaaa
      241 ggcattatcg atttcctgaa tcagtatgaa gaagcggtca aagtcgatcc gcgctcgccg
      301 caagagatga ttgatgaa
//
LOCUS       DAJVRF010000470          316 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.479, whole genome shotgun sequence.
ACCESSION   DAJVRF010000470 DAJVRF010000000
VERSION     DAJVRF010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.479"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            55..273
                     /gene="ygdR"
                     /locus_tag="OQN13_004325"
     CDS             55..273
                     /gene="ygdR"
                     /locus_tag="OQN13_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCS1617481.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT           95 a           55 c           83 g           83 t
ORIGIN      
        1 acgtcggttt ataaacagac tattatcata ggtgggccaa atgaggataa ccggatgaaa
       61 aaatgggcag taataatttc tgcagtcgga ctggcgtttg ctgtttccgg gtgttccagt
      121 gattacgtca tggcgaccaa agatggccgt atgattttga ccgatggaaa acctgaaatt
      181 gatgatgata ccgggctggt gagttatcac gatcagcaag gtaacgcgat gcaaattaac
      241 cgtgatgatg tttcgcaaat tattgaacgt taacaaataa ggtcagcatc cggctgtaat
      301 gccgatcagt taagga
//
LOCUS       DAJVRF010000471          316 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.480, whole genome shotgun sequence.
ACCESSION   DAJVRF010000471 DAJVRF010000000
VERSION     DAJVRF010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.480"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            71..146
                     /locus_tag="OQN13_004326"
     tRNA            71..146
                     /locus_tag="OQN13_004326"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:105..107,aa:Glu,seq:ttc)
BASE COUNT           86 a           72 c           75 g           83 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgata gaaagtgaaa agcaaggcgt cttgcgaagc
       61 agactgatac gtccccttcg tctagaggcc caggacaccg ccctttcacg gcggtaacag
      121 gggttcgaat cccctagggg acgccacttg ctggtttgtg agtgaaagtc acctgcctta
      181 atatctcaaa actcatcttc gggtgatgtt tgagatattt gctctttaaa aatctggatc
      241 aagctgaaaa ttgaaacact gaacaacgaa agttgttcgt gagtctctcg aattttcgca
      301 acacgatgat gaatcg
//
LOCUS       DAJVRF010000472          310 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.481, whole genome shotgun sequence.
ACCESSION   DAJVRF010000472 DAJVRF010000000
VERSION     DAJVRF010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.481"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>310
                     /locus_tag="OQN13_004327"
     CDS             <1..>310
                     /locus_tag="OQN13_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1617482.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     G"
BASE COUNT           88 a           71 c           84 g           67 t
ORIGIN      
        1 ttgaatgcaa tggcgtcgca acgaaccaaa gaggctcgtg gtggagaatt acagatcaac
       61 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      121 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      181 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      241 ttactgactc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      301 cgtgatggac
//
LOCUS       DAJVRF010000473          308 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.482, whole genome shotgun sequence.
ACCESSION   DAJVRF010000473 DAJVRF010000000
VERSION     DAJVRF010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.482"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>308
                     /gene="spy"
                     /locus_tag="OQN13_004328"
     CDS             <1..>308
                     /gene="spy"
                     /locus_tag="OQN13_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCS1617483.1"
                     /translation="PHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHD
                     IIASDTFDKAKAEAQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLT
                     "
BASE COUNT          112 a           78 c           72 g           46 t
ORIGIN      
        1 ccgcatcagg acatgatgtt caaagacctg aacctgaccg acgcgcagaa acagcagatc
       61 cgcgaaatca tgaaaggcca gcgtgaccag atgaaacgtc cgccgctgga agaacgccgc
      121 gcaatgcatg acatcattgc cagcgatacc ttcgataaag caaaagctga agcgcagatc
      181 gcaaaaatgg aagaacagcg caaagctaac atgctggcgc acatggaaac ccagaacaaa
      241 atttacaaca tcctgacgcc ggaacagaaa aagcaattta atgctaattt tgagaagcgt
      301 ctgacaga
//
LOCUS       DAJVRF010000474          308 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.483, whole genome shotgun sequence.
ACCESSION   DAJVRF010000474 DAJVRF010000000
VERSION     DAJVRF010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.483"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>308
                     /locus_tag="OQN13_004329"
     CDS             <1..>308
                     /locus_tag="OQN13_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1617484.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     P"
BASE COUNT           67 a           82 c           92 g           67 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggaccc
//
LOCUS       DAJVRF010000475          308 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.484, whole genome shotgun sequence.
ACCESSION   DAJVRF010000475 DAJVRF010000000
VERSION     DAJVRF010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.484"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            76..288
                     /gene="cspH"
                     /locus_tag="OQN13_004330"
     CDS             76..288
                     /gene="cspH"
                     /locus_tag="OQN13_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCS1617485.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT           82 a           69 c           67 g           90 t
ORIGIN      
        1 gcggtcttaa atatcgctgg ctttatttta ttaaccccgt atagtgcagc gcagtatttt
       61 cagtaaggaa tttgtttgtc ccgtaaaatg acaggaattg tcaaaacctt tgatcgcaaa
      121 agcggcaaag gattcattat cccctccgac ggtcgtaaag aagttcaggt ccatatttcc
      181 gcattcactc cccgcgacgc agaagtgctt ataccaggat tacgtgtgga attttgccgc
      241 gtaaatggcc tgcgaggacc aacagcggca aatgtttatc tctcttaata tgacgctgct
      301 tgtttaaa
//
LOCUS       DAJVRF010000476          307 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.485, whole genome shotgun sequence.
ACCESSION   DAJVRF010000476 DAJVRF010000000
VERSION     DAJVRF010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.485"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            1..307
                     /locus_tag="OQN13_004331"
                     /pseudo
     CDS             1..307
                     /locus_tag="OQN13_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          101 a           60 c           75 g           71 t
ORIGIN      
        1 ttattcgctt ctgggcatgg gcggatcaac aaatgataga cggtaatgca gattgtaacg
       61 ctcgcggcgt tacaaaaagt gcaatagatc gcatcacttt tatgtctggt tttgctgatg
      121 cgttaattca ggttggatgg ctggtcgaaa atgacggagg gctttctcta cctaactttg
      181 aacgtcataa cggaaaaagc tctaaaaaaa acgggcggtt acaaacgagc gagtaacaaa
      241 aatacgcgaa ctgaaacgaa aaggtaacgc tgccagcgtt acacaaacgg atcaaaaagc
      301 gttacca
//
LOCUS       DAJVRF010000477          304 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.486, whole genome shotgun sequence.
ACCESSION   DAJVRF010000477 DAJVRF010000000
VERSION     DAJVRF010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.486"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(119..226)
                     /gene="rprA"
                     /locus_tag="OQN13_004332"
     ncRNA           complement(119..226)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OQN13_004332"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT          107 a           48 c           68 g           81 t
ORIGIN      
        1 aaataaacaa taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca
       61 gtatcttgtg caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt
      121 aaaaaaaagc ccatcgtggg agatgggcaa agactacaca cagcaattcg ttgtttcact
      181 caggggattt ccatgcttat aaatcaatat gttgatttat aaccgtgagc taatagtagg
      241 catacggact ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt
      301 gcga
//
LOCUS       DAJVRF010000478          303 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.487, whole genome shotgun sequence.
ACCESSION   DAJVRF010000478 DAJVRF010000000
VERSION     DAJVRF010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.487"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
BASE COUNT           96 a           77 c           60 g           70 t
ORIGIN      
        1 tgacaccata acagaggcca ccctgtacga aaacgggctg tatacgcgcc acatcagact
       61 cagtcgccca gtatctgaca gcaaataccc cttgccaccc ggatctcagc tcattaaaaa
      121 tatgacctta cgactgcgcc tgcaggatca gcaggaagaa acatccgtga cattatttca
      181 gggaaaaatg agtaccgacg gaaataacta ttacatcatc aaaggtaaag taagctcagt
      241 acttctgtta ttaaaaatgc tgcagattaa tcacgcctga tctctgtccc ggtattttga
      301 ggt
//
LOCUS       DAJVRF010000479          302 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.489, whole genome shotgun sequence.
ACCESSION   DAJVRF010000479 DAJVRF010000000
VERSION     DAJVRF010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.489"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
BASE COUNT          119 a           52 c           31 g          100 t
ORIGIN      
        1 caccaaatat ccatttaatg aataaataca gtgccataaa ggtccaagta ataagcacac
       61 atttagcctg aaacattatc attcatgtca ccaaatcgtt tatttagtga gataaaacta
      121 gcatattcgt aagataaaaa atgcacatgg caatcaagaa atgatattat tacttttttg
      181 atgtaaaact atttagtttt aaaattcatt ttttcaacat atagagcatt cattttatga
      241 atgcaaaaaa acatacctga cacacaaatt actctcctct gtaatatttt ctcattaaca
      301 ct
//
LOCUS       DAJVRF010000480          302 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.denovo.490, whole genome shotgun sequence.
ACCESSION   DAJVRF010000480 DAJVRF010000000
VERSION     DAJVRF010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.denovo.490"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..>302
                     /locus_tag="OQN13_004333"
     CDS             <1..>302
                     /locus_tag="OQN13_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617486.1"
                     /translation="HGMAAYFGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSE
                     IPEGIFGKKTVELNENGVPARAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTA"
BASE COUNT           63 a           79 c           80 g           80 t
ORIGIN      
        1 ccacggcatg gcggcgtatt ttggtctacc ggaagcgttg atgaatcgct ttgtcggtct
       61 ggtgtccttt accgcgatgt tcggttccct gctgatgtgg accgcaacgc cggtgaaaat
      121 tttcttctcc gaaatcccgg aaggcatctt tggtaagaaa accgtcgaac tgaacgaaaa
      181 cggcgttccg gcgcgcgcag cgtggatcca gttcctgatc gtcatcccgc tgatgattat
      241 cccgatgctc ggttccaata ccgtgcagga tctgatgaat actattatta atatgaccgc
      301 cg
//
LOCUS       DAJVRF010000481         3996 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.guided.223, whole genome shotgun sequence.
ACCESSION   DAJVRF010000481 DAJVRF010000000
VERSION     DAJVRF010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.guided.223"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            200..673
                     /gene="dfrA"
                     /locus_tag="OQN13_004334"
     CDS             200..673
                     /gene="dfrA"
                     /locus_tag="OQN13_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA"
                     /protein_id="HCS1617487.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISRIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            768..1292
                     /gene="sat2"
                     /locus_tag="OQN13_004335"
     CDS             768..1292
                     /gene="sat2"
                     /locus_tag="OQN13_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCS1617488.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1361..1858
                     /locus_tag="OQN13_004336"
     CDS             1361..1858
                     /locus_tag="OQN13_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617489.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            1919..2290
                     /locus_tag="OQN13_004337"
     CDS             1919..2290
                     /locus_tag="OQN13_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617490.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            2700..3077
                     /locus_tag="OQN13_004338"
     CDS             2700..3077
                     /locus_tag="OQN13_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617491.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            3100..3252
                     /locus_tag="OQN13_004339"
                     /pseudo
     CDS             3100..3252
                     /locus_tag="OQN13_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3308..>3996)
                     /locus_tag="OQN13_004340"
     CDS             complement(3308..>3996)
                     /locus_tag="OQN13_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCS1617492.1"
                     /translation="RVIEAEPTWISFSRPSRIEKSRRARKSSQTILEKEEATTSENSN
                     LVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAFDELLSILKTKFACRVLFEET
                     LVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLEVDASDGVKMLSTKVLSGVDS
                     ETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGVNHPKGLGELQVSREDMEGWA
                     ERVVREQFTH"
BASE COUNT         1106 a          948 c          899 g         1043 t
ORIGIN      
        1 atgttatgag ttctttgggt gagataatgt gcatcgtgca agcaggatag acggcatgca
       61 cgatttgtaa taacagagtg tcttgtattt ttaaagaaag tctatttaat acaagtgatt
      121 atattaatta acggtaagca tcagcgggtg acaaaacgag catgcttact aataaaatgt
      181 taacctctga ggaagaattg tgaaactatc actaatggta gctatatcga agaatggagt
      241 tatcgggaat ggccctgata ttccatggag tgccaaaggt gaacagctcc tgtttaaagc
      301 tattacctat aaccaatggc tgttggttgg acgcaagact tttgaatcaa tgggagcatt
      361 acccaaccga aagtatgcgg tcgtaacacg ttcaagtttt acatctgaca atgagaacgt
      421 attgatcttt ccatcaatta aagatgcttt aaccaaccta aagaaaataa cggatcatgt
      481 cattgtttca ggtggtgggg agatatacaa aagcctgatc gatcaagtag atacactaca
      541 tatatctaga atagacatcg agccggaagg tgatgtttac tttcctgaaa tccccagcaa
      601 ttttaggcca gtttttaccc aagacttcgc ctctaacata aattatagtt accaaatctg
      661 gcaaaagggt taacaagtgg cagcaacgga ttcgcaaacc tgtcacgcct tttgtaccaa
      721 aagccgcgcc aggtttgcga tccgctgtgc caggcgttag gcgtcatatg aagatttcgg
      781 tgatccctga gcaggtggcg gaaacattgg atgctgagaa ccatttcatt gttcgtgaag
      841 tgttcgatgt gcacctatcc gaccaaggct ttgaactatc taccagaagt gtgagcccct
      901 accggaagga ttacatctcg gatgatgact ctgatgaaga ctctgcttgc tatggcgcat
      961 tcatcgacca agagcttgtc gggaagattg aactcaactc aacatggaac gatctagcct
     1021 ctatcgaaca cattgttgtg tcgcacacgc accgaggcaa aggagtcgcg cacagtctca
     1081 tcgaatttgc gaaaaagtgg gcactaagca gacagctcct tggcatacga ttagagacac
     1141 aaacgaacaa tgtacctgcc tgcaatttgt acgcaaaatg tggctttact ctcggcggca
     1201 ttgacctgtt cacgtataaa actagacctc aagtctcgaa cgaaacagcg atgtactggt
     1261 actggttctc gggagcacag gatgacgcct aacaattcat tcaagccgac accgcttcgc
     1321 ggcgcggctt aattcaggag ttagagagct ggggaagact atgcgcgatc tgttgaaggt
     1381 ggttctaagc ctcgtacttg cgatggcatc ggggcaggca cttgctgacc tgccaattgt
     1441 tttagtggat gaagctcgtc ttccctatga ctactcccca tccaactacg acatttctcc
     1501 aagcaactac gacaactcca taagcaatta cgacaatagt ccatcaaatt acgacaactc
     1561 tgagagcaac tacgataata gttcatccaa ttacgacaat agtcgcaacg gaaatcgtag
     1621 gcttatatat agcgcaaatg ggtctcgcac tttcgccggc tactacgtca ttgccaacaa
     1681 tgggacaacg aacttctttt ccacatctgg caaaaggatg ttctacaccc caaaaggggg
     1741 gcgcggcgtc tatggcggca aagatgggag cttctgcggg gcattggtcg tcataaatgg
     1801 ccaattttcg cttgccctga cagataacgg cctgaagatc atgtatctaa gcaactagcc
     1861 tgctctctaa taaaatgtta ggcctcaaca tctagtcgca agctgagggg aaccactagt
     1921 gtcatacgaa cctccaagag acggttacac aaacgggtac attgttgatg tcatgtatga
     1981 caatcgccca agtaagtatc cagctgtgtt cagaacgtac gtccgaattc cagactatgt
     2041 cgaactagat aacgcggagg agctggccga cgcaatcatt cgagaggttg agttaaagga
     2101 ggcatggctc acaagggtct tggggaactc agccgatcct gacgcttctg tgggcaagtg
     2161 ggaccggcgc ctagcgcagt gcggtggtat tgatgagatg tcaagtgggg tgcaggcaac
     2221 atacttcagt ccaaacggtc agctggtcgg cttagtggca tacattgatg ccgaatggat
     2281 ggatgaatag ctctgacgtt acgattcagg tgagacaacc agatgggcat tgcgtacaag
     2341 ctcagttgcc accgatgtgg ctacgagtct gatcttcttt acctaggcca aggtatggcg
     2401 atgcttcctg agcaaatagt cggaacgtgc acatgctgcg acaatctaac gacgatttct
     2461 ataaatgaaa gctacggagt atgctcactt tgtggggcta aggatcgtgt gatatttaca
     2521 agctatcagc atgaggcaag gccaagaaat ccctgctatc tagagagaag tttcaaatat
     2581 caatgcccaa gatgccatgc cttcagtatg gagcctccgg atctaccgga gatgctttgg
     2641 gactaatgct ccgctttgcc tcgggtatcc gttcgaccta tgtttaagag gaaacgcacg
     2701 tgtctgccgc caaagctctt gcctatgcac tttgtgacaa gttggaaagc aaaaaagttg
     2761 atgaagtcgt acgaaatctt gtttctagcg gctggaatat tagggaagtg gcctgggaag
     2821 cttcgtcctt tgatcatgaa atgccctact ttgacacggg gcgggacttc gatattggat
     2881 ttcctgacac gctgtcatcc aagattgagt ttttgaccgc gaatggatca atcgtccaag
     2941 cacgtgctcg cctgcacttc aagcaggctc taatttttgc caaggcgtcc aaacatgtcg
     3001 ccgcgctcaa gaactcattg gattactact atggatcgtc tgtaatcccc cctcaaaacc
     3061 acggaggcta tttgtagaaa attccattaa tatgaactca aggtgttccc tacggacgaa
     3121 tcagcgatga aggtggtgta cctggctatc caggcggcga ccaagaaatg gacgatgccg
     3181 atccgcaact ggaagccggc catgaaccgt tttatgatcg aatttggtga ccgtttaaat
     3241 ggccaccttt aactgaccgg caaaagcact tacacaaaag ggtttacagg ctctttcagt
     3301 cattccttta atgcgtaaat tgctctctaa ccactctctc agcccaccct tccatatcct
     3361 ctctcgatac ttgcagctcc cccaacccct ttggatgatt cacccctcta tgcccgtctt
     3421 gtccatataa ttgatcaaac aaactatttg gccaattcaa tgagctcttc accactccac
     3481 gccgtatctt ttcaaaatca ttccgccatg tttcgctatc aacgccactc aacactttgg
     3541 tagaaagcat tttcacccca tccgatgcat ccacctctag caatacaaat tcactgccat
     3601 tacgttgcac cccaacggct ttaatcactc ttggtgagcc atctttacac agatgtaatc
     3661 ggctacgccc aacttttggc aaaaccaagg tttcttcaaa aagcacccga catgcaaatt
     3721 tagtttttag aattgaaagt agctcatcaa aagcagcaaa tcgattagca aaaatagagt
     3781 tgtaattggt tgcatcctgc ttcccaccca catctgccgc tgctaggaca ccacctaagt
     3841 gtggctcatc agtactaacc aaattactat tttcacttgt tgttgcttct tctttttcta
     3901 aaatagtttg gctacttttt cttgccctgc gagatttttc aattcgacta ggtctactaa
     3961 aacttatcca tgtcggctca gcctctatga ctcgtg
//
LOCUS       DAJVRF010000482         3879 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.guided.227, whole genome shotgun sequence.
ACCESSION   DAJVRF010000482 DAJVRF010000000
VERSION     DAJVRF010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.guided.227"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            996..1811
                     /gene="sul2"
                     /locus_tag="OQN13_004341"
     CDS             996..1811
                     /gene="sul2"
                     /locus_tag="OQN13_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS1617493.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1872..2675
                     /locus_tag="OQN13_004342"
                     /note="aph(3'')-Ib"
     CDS             1872..2675
                     /locus_tag="OQN13_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCS1617494.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCQFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2675..3511
                     /locus_tag="OQN13_004343"
     CDS             2675..3511
                     /locus_tag="OQN13_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS1617495.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            3817..>3879
                     /locus_tag="OQN13_004344"
     CDS             3817..>3879
                     /locus_tag="OQN13_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HCS1617496.1"
                     /translation="MKTAALDLARERQAHEAGART"
BASE COUNT          834 a         1092 c         1072 g          881 t
ORIGIN      
        1 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtaccgg
       61 cttacggacg gggacggaaa tttcctggaa gataccagga agacacttag caaagaagag
      121 ataaggccac ggcgaagccg tttttccata ggctccgccc ccctgacaag catcgcaaaa
      181 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatcc caggcgtttc
      241 cccctggtag ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
      301 cgctgttatg gccgcggttt ctcattccac gcctgacact cagttccgtg aaggcagttc
      361 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttgtccg
      421 gtaactatca acttgagtcc aacccggaaa gacacgacaa aacgccactg gcagcagcca
      481 ttagtaacag gagtgcacga gaagatagat taagaggtct tgaagtagag gcctgactgc
      541 ggctacactg aaagaacagt tttggtaatg gcggtctcgt gaaccctgtt accgggttaa
      601 gcagttcccc aactgactta accttcgaac aaaccgcctc cccaggcggt tttttcgttt
      661 acagagcaag agattacgat tcattgaaaa ggatctcaag aagatccttt gtagattact
      721 atcaattaat cttccgaaca tgacaccagt cactgttgat tacggttaaa ccgctccgta
      781 acaggtttcc ctcgtattgg tgacttgcag caccaaagaa gcaaaaaaga taaatatatc
      841 tacttattga gagatgatat atttatttcc aaaatgctac agatacatca tcatgaaacg
      901 gatcaccgca aacaggttac tcgcctgggg attccctttc gacccgagca tccgtatgag
      961 actcatgctc gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg
     1021 gcatcgtcaa cataacctcg gacagtttct ccgatggagg ccggtatctg gcgccagacg
     1081 cagccattgc gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc gacctcggtc
     1141 cggcatccag caatcccgac gccgcgcctg tttcgtccga cacagaaatc gcgcgtatcg
     1201 cgccggtgct ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac agttatcaac
     1261 ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt
     1321 ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc
     1381 attcggtgca agacgggcag gcagatcggc gcgaggcacc cgctggcgac atcatggatc
     1441 acattgcggc gttctttgac gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca
     1501 accgccttgt ccttgatccc ggcatggggt tttttctggg ggctgctccc gaaacctcgc
     1561 tctcggtgct ggcgcggttc gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt
     1621 ctgtttcgcg caaatccttt ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg
     1681 ccgcgacact cgctgcagag cttgccgccg ccgcaggtgg agctgacttc atccgcacac
     1741 acgagccgcg ccccttgcgc gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa
     1801 gaattcgtta actgcacatt cgggatattt ctctatattc gcgcttcatc agaaaactga
     1861 aggaacctcc attgaatcga actaatattt tttttggtga atcgcattct gactggttgc
     1921 ctgtcagagg cggagaatct ggtgattttg tttttcgacg tggtgacggg catgccttcg
     1981 cgaaaatcgc acctgcttcc cgccgcggtg agctcgctgg agagcgtgac cgcctcattt
     2041 ggctcaaagg tcgaggtgtg gcttgccccg aggtcatcaa ctggcaggag gaacaggagg
     2101 gtgcatgctt ggtgataacg gcaattccgg gagtaccggc ggctgatctg tctggagcgg
     2161 atttgctcaa agcgtggccg tcaatggggc agcaacttgg cgctgttcac agcctatcgg
     2221 ttgatcaatg tcagtttgag cgcaggctgt cgcgaatgtt cggacgcgcc gttgatgtgg
     2281 tgtcccgcaa tgccgtcaat cccgacttct taccggacga ggacaagagt acgccgcagc
     2341 tcgatctttt ggctcgtgtc gaacgagagc taccggtgcg gctcgaccaa gagcgcaccg
     2401 atatggttgt ttgccatggt gatccctgca tgccgaactt catggtggac cctaaaactc
     2461 ttcaatgcac gggtctgatc gaccttgggc ggctcggaac agcagatcgc tatgccgatt
     2521 tggcactcat gattgctaac gccgaagaga actgggcagc gccagatgaa gcagagcgcg
     2581 ccttcgctgt cctattcaat gtattgggga tcgaagcccc cgaccgcgaa cgccttgcct
     2641 tctatctgcg attggaccct ctgacttggg gttgatgttc atgccgcctg tttttcctgc
     2701 tcattggcac gtttcgcaac ctgttctcat tgcggacacc ttttccagcc tcgtttggaa
     2761 agtttcattg ccagacggga ctcctgcaat cgtcaaggga ttgaaaccta tagaagacat
     2821 tgctgatgaa ctgcgcgggg ccgactatct ggtatggcgc aatgggaggg gagcagtccg
     2881 gttgctcggt cgtgagaaca atctgatgtt gctcgaatat gccggggagc gaatgctctc
     2941 tcacatcgtt gccgagcacg gcgactacca ggcgaccgaa attgcagcgg aactaatggc
     3001 gaagctgtat gccgcatctg aggaacccct gccttctgcc cttctcccga tccgggatcg
     3061 ctttgcagct ttgtttcagc gggcgcgcga tgatcaaaac gcaggttgtc aaactgacta
     3121 cgtccacgcg gcgattatag ccgatcaaat gatgagcaat gcctcggaac tgcgtgggct
     3181 acatggcgat ctgcatcatg aaaacatcat gttctccagt cgcggctggc tggtgataga
     3241 tcccgtcggt ctggtcggtg aagtgggctt tggcgccgcc aatatgttct acgatccggc
     3301 tgacagagac gacctttgtc tcgatcctag acgcattgca cagatggcgg acgcattctc
     3361 tcgtgcgctg gacgtcgatc cgcgtcgcct gctcgaccag gcgtacgctt atgggtgcct
     3421 ttccgcagct tggaacgcgg atggagaaga ggagcaacgc gatctagcta tcgcggccgc
     3481 gatcaagcag gtgcgacaga cgtcatacta gatatcaagc gacttctcct atcccctggg
     3541 aacacatcaa tctcaccgga gaatatcgct ggccaaagcc ttagcgtagg attccgcccc
     3601 ttcccgcaaa cgacccctgg aagccccgta gcgacgcgga gaggggcgag acaagccaag
     3661 ggcgcaggct cgatgcgcag cacgacatag ccggttctcg caaggacgag aatttccctg
     3721 cggtgcccct caagtgtcaa tgaaagtttc caacgcgagc cattcgcgag agccttgagt
     3781 ccacgctaga tctatctcat ctgcgcaagg cagaacgtga agacggccgc cctggacctc
     3841 gcccgcgagc gccaggcgca cgaggccggc gcgcggacc
//
LOCUS       DAJVRF010000483         3794 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.guided.230, whole genome shotgun sequence.
ACCESSION   DAJVRF010000483 DAJVRF010000000
VERSION     DAJVRF010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.guided.230"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            complement(180..830)
                     /gene="tetR(A)"
                     /locus_tag="OQN13_004345"
     CDS             complement(180..830)
                     /gene="tetR(A)"
                     /locus_tag="OQN13_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCS1617497.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            936..2135
                     /gene="tet(A)"
                     /locus_tag="OQN13_004346"
     CDS             936..2135
                     /gene="tet(A)"
                     /locus_tag="OQN13_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCS1617498.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            complement(2102..2290)
                     /locus_tag="OQN13_004347"
                     /pseudo
     CDS             complement(2102..2290)
                     /locus_tag="OQN13_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(2334..2723)
                     /locus_tag="OQN13_004348"
     CDS             complement(2334..2723)
                     /locus_tag="OQN13_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1617499.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            2941..3381
                     /locus_tag="OQN13_004349"
     CDS             2941..3381
                     /locus_tag="OQN13_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCS1617500.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT          803 a         1076 c         1067 g          848 t
ORIGIN      
        1 catcgtcggc agcgtcagat gtgtataaga gacaggccgt gaggcttgga cgctagggca
       61 gggcatgaaa aagcccgtag cgggctgcta cgggcgtctg acgcggtgga aagggggagg
      121 ggatgttgtc tacatggctc tgctgtagtg agtgggttgc gctccggcag cggtcctgat
      181 caatcgtcac cctttctcgg tccttcaacg ttcctgacaa cgagcctcct tttcgccaat
      241 ccatcgacaa tcaccgcgag tccctgctcg aacgctgcgt ccggaccggc ttcgtcgaag
      301 gcgtctatcg cggcccgcaa cagcggcgag agcggagcct gttcaacggt gccgccgcgc
      361 tcgccggcat cgctgtcgcc ggcctgctcc tcaagcacgg ccccaacagt gaagtagctg
      421 attgtcatca gcgcattgac ggcgtccccg gccgaaaaac ccgcctcgca gaggaagcga
      481 agctgcgcgt cggccgtttc catctgcggt gcgcccggtc gcgtgccggc atggatgcgc
      541 gcgccatcgc ggtaggcgag cagcgcctgc ctgaagctgc gggcattccc gatcagaaat
      601 gagcgccagt cgtcgtcggc tctcggcacc gaatgcgtat gattctccgc cagcatggct
      661 tcggccagtg cgtcgagcag cgcccgcttg ttcctgaagt gccagtaaag cgccggctgc
      721 tgaaccccca accgttccgc cagtttgcgt gtcgtcagac cgtctacgcc gacctcgttc
      781 aacaggtcca gggcggcacg gatcactgta ttcggctgca actttgtcat gcttgacact
      841 ttatcactga taaacataat atgtccacca acttatcagt gataaagaat ccgcgcgttc
      901 aatcggacca gcggaggctg gtccggaggc cagacgtgaa acccaacaga cccctgatcg
      961 taattctgag cactgtcgcg ctcgacgctg tcggcatcgg cctgattatg ccggtgctgc
     1021 cgggcctcct gcgcgatctg gttcactcga acgacgtcac cgcccactat ggcattctgc
     1081 tggcgctgta tgcgttgatg caatttgcct gcgcacctgt gctgggcgcg ctgtcggatc
     1141 gtttcgggcg gcggccggtc ttgctcgtct cgctggccgg cgctgctgtc gactacgcca
     1201 tcatggcgac ggcgcctttc ctttgggttc tctatatcgg gcggatcgtg gccggcatca
     1261 ccggggcgac tggggcggta gccggcgctt atattgccga tatcactgat ggcgatgagc
     1321 gcgcgcggca cttcggcttc atgagcgcct gtttcgggtt cgggatggtc gcgggacctg
     1381 tgctcggtgg gctgatgggc ggtttctccc cccacgctcc gttcttcgcc gcggcagcct
     1441 tgaacggcct caatttcctg acgggctgtt tccttttgcc ggagtcgcac aaaggcgaac
     1501 gccggccgtt acgccgggag gctctcaacc cgctcgcttc gttccggtgg gcccggggca
     1561 tgaccgtcgt cgccgccctg atggcggtct tcttcatcat gcaacttgtc ggacaggtgc
     1621 cggccgcgct ttgggtcatt ttcggcgagg atcgctttca ctgggacgcg accacgatcg
     1681 gcatttcgct tgccgcattt ggcattctgc attcactcgc ccaggcaatg atcaccggcc
     1741 ctgtagccgc ccggctcggc gaaaggcggg cactcatgct cggaatgatt gccgacggca
     1801 caggctacat cctgcttgcc ttcgcgacac ggggatggat ggcgttcccg atcatggtcc
     1861 tgcttgcttc gggtggcatc ggaatgccgg cgctgcaagc aatgttgtcc aggcaggtgg
     1921 atgaggaacg tcaggggcag ctgcaaggct cactggcggc gctcaccagc ctgacctcga
     1981 tcgtcggacc cctcctcttc acggcgatct atgcggcttc tataacaacg tggaacgggt
     2041 gggcatggat tgcaggcgct gccctctact tgctctgcct gccggcgctg cgtcgcgggc
     2101 tttggagaaa ttcttcaaat tcccgttgca catagcccgg caattccttt ccctgctctg
     2161 ccataagcgc agcgaatgcc gggtaatact cgtcaacgat ctgatagaga agggtttgct
     2221 cgggtcggtg gctctggtaa cgaccagtat cccgatcccg gctggccgtc ctggccgcca
     2281 catgaggcat gttccgcgtc cttgcaatac tgtgtttaca tacagtctat cgcttagcgg
     2341 aaagttcttt taccctcagc cgaaatgcct gccgttgcta gacattgcca gccagtgccc
     2401 gtcactccca ctcttacatt gttcgcagta tctaggtaca tgttattgcg tgcaccttca
     2461 tgatgtataa tgttattaat gacagcagaa agatcgacag aattaatatt cttcgtacct
     2521 gggacagtta tcgggtgtcg gccagtgatg agaccaaagg atgcagtata agatgaacca
     2581 ctactattat catggccacc attaccacca ccgttaaaac tacctcccga acttccagag
     2641 ccgctatgac catcattgta ggggtcattt ggtcccatgt gaccaaaacc accgacagta
     2701 ccgctattta actctgtact cataaaatta tcctctttga cttttaaaaa cagcaaaaac
     2761 tgtatttata acaatgtgtt tatatacagt attcgaaata ctacgcccgt caaaaccaca
     2821 ccgtcaacag gttttttgtg tatttttcac gcctcacggg cagcctcaga ccttttgtgt
     2881 taaaacagta atatgttctg ctgttacaat acggtacaca caggagacac tgtcactttt
     2941 atggcatggc acacagttca agagacactg aaattaacag gaaagtcgcg ctcgcaatta
     3001 tatcgtgaca tggctaaagg ccttgtcagc tatcgcacag gaaaaaatga tcgccgggag
     3061 ttcgagacca gtgaactgat tcgggcctat ggcgagctgc gcacttctga gacaccaaaa
     3121 aggcacaagg agggacactc caaatctgtc gttagccagg cggaaaatgc tcagctagac
     3181 gtcattcagc agaaactgga cgctctgcag cagactgtta ccctaatgct tgaagacaag
     3241 atggcgcggg aagcagaaaa ccgcctgcga aatgaggaga gggaacaact acaggccgaa
     3301 attagccgtc tggctgacgc gctggcacag gaaaaaaagc ggggattctg gtcgcggctg
     3361 tttcgctccg gttcagagta acagtgcagg tgactctgat ttaccataac atctgttatc
     3421 agcaccgcct gagaggccct cagggcgttt ctgacgggaa accggtaaaa caggcaacaa
     3481 accacccgaa aacccgccag aaacgcgcag gctctgtttt tacggcatgc atgagtatgc
     3541 gcacaagtgc atagcgcgga aattcacacc attcagccgg ggtaagtcct gtttccggcg
     3601 ttcttcccat ccgaaaacgg tgaacgggga cgcaatttat tgcgtggccg tgaagcgttg
     3661 ccggcgcagc cggaaaaccc gctgtcagca cgttcaggaa gggggtgatg tacaaatggc
     3721 acagaggatg aaagtgacgt atgagctgaa ggaatgatgt aaaagtgatg tataagtgat
     3781 gtaaaagtga tgta
//
LOCUS       DAJVRF010000484         3269 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.guided.242, whole genome shotgun sequence.
ACCESSION   DAJVRF010000484 DAJVRF010000000
VERSION     DAJVRF010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.guided.242"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            <1..234
                     /locus_tag="OQN13_004350"
     CDS             <1..234
                     /locus_tag="OQN13_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HCS1617501.1"
                     /translation="RKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEYSDV
                     KITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            227..961
                     /gene="frdB"
                     /locus_tag="OQN13_004351"
     CDS             227..961
                     /gene="frdB"
                     /locus_tag="OQN13_004351"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCS1617502.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            972..1367
                     /gene="frdC"
                     /locus_tag="OQN13_004352"
     CDS             972..1367
                     /gene="frdC"
                     /locus_tag="OQN13_004352"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCS1617503.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            1378..1737
                     /gene="frdD"
                     /locus_tag="OQN13_004353"
     CDS             1378..1737
                     /gene="frdD"
                     /locus_tag="OQN13_004353"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS1617504.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            1800..2933
                     /locus_tag="OQN13_004354"
     CDS             1800..2933
                     /locus_tag="OQN13_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS1617505.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            3022..>3269
                     /gene="blc"
                     /locus_tag="OQN13_004355"
     CDS             3022..>3269
                     /gene="blc"
                     /locus_tag="OQN13_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCS1617506.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPD"
BASE COUNT          804 a          847 c          851 g          767 t
ORIGIN      
        1 cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag gttgcaccga gcgtgacgac
       61 gtcaacttcc tcaaacacac cctcgccttc cgcgatgctg atggcacgac tcgcctagag
      121 tacagcgacg tgaagattac tacgctgccg ccagctaaac gcgtttacgg tggcgaagcg
      181 gatgcagccg ataaggcgga agcagccaat aagaaggaga aggcgaatgg ctgagatgaa
      241 aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac cgcatagcgc
      301 attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg tctacatcaa
      361 agacaacctg gcaccggacc tgagttaccg ctggtcctgc cgtatggcga tttgtggttc
      421 ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc tgcgtgatta
      481 caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacgcg atctggtggt
      541 cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca tcggcaactc
      601 ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcaa agtatcacca
      661 gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt ttggcctgaa
      721 cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg aagatagccg
      781 cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggcg tatggagctg
      841 tactttcgtg ggctattgct ccgaagtctg cccgaaacac gtcgatccgg ctgcggccat
      901 tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga aaccacgcta
      961 aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg tccacctggt
     1021 ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg gttccggctg
     1081 tgtggttcag cattgaactg attttcgggc tgtttgccct gaaaaatggc ccggaagcct
     1141 gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt gatcattaac ctgatcactc
     1201 tggcggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa gcggccaata
     1261 tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc tgggcggtaa
     1321 ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga gcctgagatg
     1381 attaatccaa atccaaagcg ttctgacgaa ccggtattct ggggcctctt cggggccggt
     1441 ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggtgggtat tctgctgcca
     1501 ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg ttctggcgtt cgcgcagagc
     1561 ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg tggtttacac
     1621 cgtatgcacc acgcgatgca cgatctgaaa attcacgtgc ctgcgggcaa atgggttttc
     1681 tacggtctgg ctgctatcct gacagttgtc acgctgattg gtatcgttac aatctaacgt
     1741 atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa ccagacctta
     1801 tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttgctccaca tttgctgccc
     1861 ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag caacaaaaga
     1921 tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt acctggggct
     1981 atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag ttaggttcgg
     2041 tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg gaaatcaagt
     2101 taagcgatcc cgcaacaaaa tactggcctg aacttactgc taaacagtgg aatgggatca
     2161 cactattaca tcttgcgacc tacaccgctg gcggcctgcc attgcaggtg ccggatgaag
     2221 tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca tgggctccag
     2281 gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg gctgtgaagc
     2341 cgtctggttt gagttttgag caggcgatgc aaactcgtgt cttccagcca ctcaaactca
     2401 accatacgtg gattaatgta cctcccccag aagaaaagaa ttacgcctgg ggatatcgcg
     2461 aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc tgaagcttat ggtgtgaagt
     2521 cgaccattga agatatggcc cgctgggtgc aaagcaattt aacccccctt gatatcaatg
     2581 agaaaacgct tcaacaaggg atacaactgg cacaatctcg ctactggcaa accggcgata
     2641 tgtatcaggg cctgggctgg gaaatgctgg actggccggt caatcctgac agcatcatta
     2701 acggcagtga caataaaatt gcactggcag cacgcccggt aaaagcgatt aagcccccaa
     2761 ctcctgcagt acgcgcatca tgggtacata aaacgggggc gaccggcgga tttggtagct
     2821 atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat gctggctaac aaaaactatc
     2881 ccaatccagc gagagtcgcc gccgcctgtc agattctcaa cgctctacag taaaatttca
     2941 tcgggtccga attttcggac cttttctgcg cttttccttg ctgtcatcta cacttagaaa
     3001 aaaaccagta aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc gacagctgca
     3061 tttctggtcg ttgcctgcag ttctcctacg ccgccgcgtg gcgtgacagt agtgaataat
     3121 ttcgacgcta aacgctatct cggtacctgg tatgagattg cccgttttga tcaccgtttt
     3181 gaacgtggac tggaaaaagt caccgcaaca tacagtctgc gtgatgacgg cggcctgaat
     3241 gtcatcaata aaggctataa ccctgacag
//
LOCUS       DAJVRF010000485         1355 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 503019
            SAMEA8900175-rid15854493.guided.301, whole genome shotgun sequence.
ACCESSION   DAJVRF010000485 DAJVRF010000000
VERSION     DAJVRF010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900175
            Sequence Read Archive: ERR6005522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478436.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478436.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,357
            CDSs (total)                      :: 4,275
            Genes (coding)                    :: 4,024
            CDSs (with protein)               :: 4,024
            Genes (RNA)                       :: 82
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 119 of 251
            Pseudo Genes (incomplete)         :: 71 of 251
            Pseudo Genes (internal stop)      :: 85 of 251
            Pseudo Genes (multiple problems)  :: 24 of 251
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900175-rid15854493.guided.301"
                     /serovar="S. sonnei"
                     /isolate="503019"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="India"
                     /collection_date="2009-03-02"
     gene            101..559
                     /gene="gpt"
                     /locus_tag="OQN13_004356"
     CDS             101..559
                     /gene="gpt"
                     /locus_tag="OQN13_004356"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS1617507.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            651..>1355
                     /locus_tag="OQN13_004357"
     CDS             651..>1355
                     /locus_tag="OQN13_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1617508.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGF"
BASE COUNT          307 a          349 c          363 g          336 t
ORIGIN      
        1 aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa
       61 cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt
      121 cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga
      181 acaatggaaa ggcattattg ccgtaagccg tggcggtctg gtaccgggtg cgttactggc
      241 gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa
      301 ccagcgcgag cttaaagtgc taaaacgcgc agaaggcgat ggcgaaggct tcatcgttat
      361 tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc
      421 gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt
      481 tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc
      541 gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa
      601 cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg
      661 caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag
      721 tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca
      781 tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac
      841 gtgaaatcct cgacgtccag gcgcgtattg tgatgagcga tgccgaacgt accgacgatg
      901 atttatacga tacggtgatt ggctaccgtg gtggcaactg gatttatgag tgggccatcc
      961 aggcgatggt gtggcaacaa aaagcctgtg cggaagaaga tccgcaactc agtggtcgtc
     1021 actggctgca tgcggctacg ttgtacaaca ttgctgccta tcctcatctg aaaggagatg
     1081 acctggccga gcaagcgcag gctttgtcaa accgtgccta tgaagaagcc gcccagcgtc
     1141 tgccgggcac gatgcggcag atggagttta ccgtacccgg cggtgcgccc atcaccggct
     1201 ttttgcatat gccgaaaggc gatggcccgt tcccgacagt attaatgtgt ggtggtctgg
     1261 atgcgatgca gacggactat tacagcctgt atgaacgtta ttttgcgccg cgcggcattg
     1321 cgatgctgac tattgatatg ccgtcggtgg gcttt
//
